MAP4K4 gene cDNA ORF clone, Tursiops truncatus(common bottlenose dolphin)

mRNA	Protein	Name
XM_019931224.1	XP_019786783.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X7
XM_019931230.1	XP_019786789.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X13
XM_019931223.1	XP_019786782.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X6
XM_019931228.1	XP_019786787.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X11
XM_019931231.1	XP_019786790.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X14
XM_019931234.1	XP_019786793.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X17
XM_019931218.1	XP_019786777.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X1
XM_019931221.1	XP_019786780.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X4
XM_019931227.1	XP_019786786.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X10
XM_019931229.1	XP_019786788.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X12
XM_019931232.1	XP_019786791.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X15
XM_019931233.1	XP_019786792.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X16
XM_019931219.1	XP_019786778.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X2
XM_019931220.1	XP_019786779.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X3
XM_019931222.1	XP_019786781.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X5
XM_019931226.1	XP_019786785.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X9
XM_019931225.1	XP_019786784.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X8

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTu39192	XM_019931233.1 Latest version!	Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OTu39177	XM_019931218.1 Latest version!	Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OTu39178	XM_019931219.1 Latest version!	Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OTu39179	XM_019931220.1 Latest version!	Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OTu39180	XM_019931221.1 Latest version!	Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OTu39181	XM_019931222.1 Latest version!	Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OTu39182	XM_019931223.1 Latest version!	Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OTu39183	XM_019931224.1 Latest version!	Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OTu39184	XM_019931225.1 Latest version!	Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OTu39185	XM_019931226.1 Latest version!	Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OTu39186	XM_019931227.1 Latest version!	Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OTu39187	XM_019931228.1 Latest version!	Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OTu39188	XM_019931229.1 Latest version!	Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OTu39189	XM_019931230.1 Latest version!	Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OTu39190	XM_019931231.1 Latest version!	Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OTu39191	XM_019931232.1 Latest version!	Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OTu39193	XM_019931234.1 Latest version!	Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

MAP4K4 ( XM_019931233.1 ) cDNA ORF clone, Tursiops truncatus(common bottlenose dolphin) -> XP_019786792.1 Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OTu39192
Clone ID Related Accession (Same CDS sequence)	XM_019931233.1
Accession Version	XM_019931233.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Tursiops truncatus(common bottlenose dolphin)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017842906.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tursiops truncatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
CCGGCTGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
AAGGGTCGAC ACGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA CGTTACTGAG 
GATGAAGAGG AGGAAATCAA GCTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGCGGGGCC GGGTCCATCA CAGACCTCGT GAAGAACACC 
AAGGGCAACA CGCTCAAGGA GGACTGGATA GCCTACATCT CCAGGGAGAT CCTGAGGGGA 
CTGGCGCATC TCCACATCCA TCACGTGATT CACCGGGACA TCAAGGGCCA GAATGTATTG 
CTGACCGAGA ACGCAGAGGT GAAGCTTGTG GATTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACCGTGGGGA GGAGAAACAC CTTCATAGGC ACCCCGTACT GGATGGCTCC CGAGGTCATC 
GCCTGTGACG AGAACCCCGA CGCCACCTAT GATTACAGGA GTGACCTGTG GTCCTGCGGC 
ATCACAGCCA TTGAGATGGC AGAAGGCGCT CCCCCTCTCT GTGACATGCA TCCCATGAGA 
GCACTGTTTC TCATCCCCAG AAACCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCAAAA 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACA 
GAGCAGCTTC TGAAACATCC TTTTATCAGG GACCAGCCGA ATGAAAGGCA GGTTAGGATC 
CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACCGAG 
TACGAGTACA GCGGGAGTGA GGAGGAGGAG GAGGAGGCGC CGGAGCAGGA GGGGGAGCCC 
AGCTCCATCG TCAACGTGCC TGGCGAGTCC ACGCTGCGCC GGGACTTCCT GAGGCTGCAG 
CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGCGGCAGC AGCTCCTGCA GGAGCAGCAG 
CTCAGGGAGC AGGAAGAATA CAAGAGGCAG CTGCTGGCGG AGAGACAGAA GCGGATCGAG 
CAGCAGAAGG AGCAGCGGCG ACGGCTGGAA GAGCAACAAA GGAGAGAGCG GGAGGCCAGG 
AGGCAGCAGG AGCGCGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
CTGGAGAGAC GGCGGCAGGA GGAAGAGGAG CGCAGGCGGG CGGAGGAGGA CAAGAGGAGA 
GTCGACAGGG AGCAGGTTCC TGTGAGAACG ACGTCCCGGT CCCCCGTCCT GTCCCGTCGG 
GATTCCCCAC TGCAGGGCAG TGGGCAGCAA AACAGCCAAG CAGGTCAGAG AAACTCCACC 
AGCAGCATCG AGCCCAGGCT GCTATGGGAG AGAGTGGAGA AGCTGGTGCC CAGGCCTGGC 
AGTGGCAGCT CCTCGGGGTC CAGCAACTCA GGATCCCAGC CCGGCTCCCA CCCTGGGTCT 
CAGAGCGGCT CCGGGGAGCG CTTCAGAGTG AGATCGTCAT CCAAATCTGA GGGCTCGCCA 
TCTCAGCGCC TTGAAAATGC AGCAAAAAAG CCTGAAGATA AGAAGGAAGT TTTTAGACCT 
CTCAAGCCCG CTGGCGAAGT GGACCTGACG GCCCTGGCCA GAGAGCTCCG AGCGGCGGAA 
GACGTGCGGC CGCCGCACAA GGTGACAGAC TACTCGTCGT CCAGCGAGGA GTCCGGGACA 
ACGGATGAGG AGGACGACGA CGTGGAGCAG GAAGGCCCCG AGGAGCCCAC CTCGGGGCCG 
GAGGACGCCC GAGCCGCGTC ATCTCTGAAC TTGAGCAACG GTGAGACGGA GTCCGTGAAA 
ACGATGATCG TCCACGACGA CGTGGAGAGT GAACCAGCCA TGACCCCGTC CAAGGAGGGC 
ACCCTGATTG TCCGCCAGAG TGCAGTTGAC CAAAAGCGCG CCAGCCATCA TGAGAGCAAT 
GGCTTTGCCG GTCGCGTTCA CCTCTTGCCA GATCTCTTAC AGCAAAGCCA TTCCTCCTCC 
ACTTCCTCCA CCTCCTCCTC CCCATCCTCC AGCCAGCCGA CACCCACCAT GTCCCCACAG 
ACACCCCAGG ACAAGCTCAC TCCTAACGAG ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA 
CACAAATCTT CCTCCTCCTT TACACCTTTT ATAGACCCCA GATTACTACA GATTTCTCCA 
TCTAGTGGGA CAACAGTGAC TTCTGTGGTG GGATTTTCCT GTGATGGAAT GAGATCGGAG 
GCCATAAGGC AAGATCCTAC CCGGAAGGGC TCAGTGGTCA ATGTGAATCC CACCAACACT 
AGGCCACAGA GCGACACCCC AGAGATTCGC AAATATAAGA AGAGGTTTAA CTCCGAGATT 
CTGTGCGCTG CCTTGTGGGG AGTGAACTTG TTGGTGGGTA CAGAGAGTGG CCTGATGCTG 
CTGGACAGAA GTGGCCAAGG GAAGGTGTAT CCTCTGATCA ACAGAAGACG ATTTCAACAG 
ATGGACGTCC TGGAAGGCTT GAATGTCTTG GTGACAATAT CTGGCAAAAA GGATAAGTTA 
CGTGTCTACT ATTTGTCCTG GTTAAGAAAT AAAATACTTC ACAACGATCC GGAAGTTGAG 
AAGAAGCAGG GTTGGACGAC CGTGGGAGAT TTGGAAGGAT GCGTCCACTA TAAAGTCGTA 
AAATATGAAA GAATCAAGTT TCTGGTCATT GCTTTGAAGA GTTCTGTGGA AGTGTATGCG 
TGGGCACCCA AGCCATATCA CAAATTCATG GCCTTTAAGT CGTTTGGAGA TTTGGTGCAT 
AAGCCATTAC TGGTGGATCT CACTGTTGAG GAAGGCCAGA GGTTGAAAGT GATCTATGGA 
TCCTGTGCTG GATTCCATGC TGTTGACGTG GATTCAGGAT CAGTCTATGA CATTTATCTA 
CCAACACACG TAAGAAAGAA CCCACACTCT ATGATCCAGT GCAGCATCAA ACCCCACGCA 
ATCATCATCC TCCCCAACAC GGATGGAATG GAGCTTCTGG TGTGCTATGA AGACGAAGGG 
GTCTATGTGA ACACCTATGG AAGGATTACC AAGGATGTGG TTCTGCAGTG GGGAGAGATG 
CCAACGTCTG TAGCGTATAT TCGATCCAAT CAGACGATGG GCTGGGGAGA AAAGGCCATA 
GAGATCCGGT CTGTGGAGAC GGGCCACTTG GACGGTGTGT TTATGCACAA AAGGGCTCAA 
AGACTGAAAT TCTTATGTGA ACGCAACGAC AAGGTGTTCT TTGCCTCGGT CCGGTCCGGG 
GGCAGCAGCC AGGTGTACTT CATGACGCTG GGCAGGACCT CTCTTCTGAG CTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019786792.1
CDS	146..3622
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEAPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRQEEEERRRAEEDKRRVDREQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPAGEVDLTALARELRAAEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGPEEPTSGPEDARAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSAVDQKRASHHESNGFAGRVHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTPNETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRSEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGDLVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_019931233.1
Organism	Tursiops truncatus(common bottlenose dolphin)
Definition	Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019931233.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
CCGGCTGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
AAGGGTCGAC ACGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA CGTTACTGAG 
GATGAAGAGG AGGAAATCAA GCTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGCGGGGCC GGGTCCATCA CAGACCTCGT GAAGAACACC 
AAGGGCAACA CGCTCAAGGA GGACTGGATA GCCTACATCT CCAGGGAGAT CCTGAGGGGA 
CTGGCGCATC TCCACATCCA TCACGTGATT CACCGGGACA TCAAGGGCCA GAATGTATTG 
CTGACCGAGA ACGCAGAGGT GAAGCTTGTG GATTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACCGTGGGGA GGAGAAACAC CTTCATAGGC ACCCCGTACT GGATGGCTCC CGAGGTCATC 
GCCTGTGACG AGAACCCCGA CGCCACCTAT GATTACAGGA GTGACCTGTG GTCCTGCGGC 
ATCACAGCCA TTGAGATGGC AGAAGGCGCT CCCCCTCTCT GTGACATGCA TCCCATGAGA 
GCACTGTTTC TCATCCCCAG AAACCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCAAAA 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACA 
GAGCAGCTTC TGAAACATCC TTTTATCAGG GACCAGCCGA ATGAAAGGCA GGTTAGGATC 
CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACCGAG 
TACGAGTACA GCGGGAGTGA GGAGGAGGAG GAGGAGGCGC CGGAGCAGGA GGGGGAGCCC 
AGCTCCATCG TCAACGTGCC TGGCGAGTCC ACGCTGCGCC GGGACTTCCT GAGGCTGCAG 
CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGCGGCAGC AGCTCCTGCA GGAGCAGCAG 
CTCAGGGAGC AGGAAGAATA CAAGAGGCAG CTGCTGGCGG AGAGACAGAA GCGGATCGAG 
CAGCAGAAGG AGCAGCGGCG ACGGCTGGAA GAGCAACAAA GGAGAGAGCG GGAGGCCAGG 
AGGCAGCAGG AGCGCGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
CTGGAGAGAC GGCGGCAGGA GGAAGAGGAG CGCAGGCGGG CGGAGGAGGA CAAGAGGAGA 
GTCGACAGGG AGCAGGTTCC TGTGAGAACG ACGTCCCGGT CCCCCGTCCT GTCCCGTCGG 
GATTCCCCAC TGCAGGGCAG TGGGCAGCAA AACAGCCAAG CAGGTCAGAG AAACTCCACC 
AGCAGCATCG AGCCCAGGCT GCTATGGGAG AGAGTGGAGA AGCTGGTGCC CAGGCCTGGC 
AGTGGCAGCT CCTCGGGGTC CAGCAACTCA GGATCCCAGC CCGGCTCCCA CCCTGGGTCT 
CAGAGCGGCT CCGGGGAGCG CTTCAGAGTG AGATCGTCAT CCAAATCTGA GGGCTCGCCA 
TCTCAGCGCC TTGAAAATGC AGCAAAAAAG CCTGAAGATA AGAAGGAAGT TTTTAGACCT 
CTCAAGCCCG CTGGCGAAGT GGACCTGACG GCCCTGGCCA GAGAGCTCCG AGCGGCGGAA 
GACGTGCGGC CGCCGCACAA GGTGACAGAC TACTCGTCGT CCAGCGAGGA GTCCGGGACA 
ACGGATGAGG AGGACGACGA CGTGGAGCAG GAAGGCCCCG AGGAGCCCAC CTCGGGGCCG 
GAGGACGCCC GAGCCGCGTC ATCTCTGAAC TTGAGCAACG GTGAGACGGA GTCCGTGAAA 
ACGATGATCG TCCACGACGA CGTGGAGAGT GAACCAGCCA TGACCCCGTC CAAGGAGGGC 
ACCCTGATTG TCCGCCAGAG TGCAGTTGAC CAAAAGCGCG CCAGCCATCA TGAGAGCAAT 
GGCTTTGCCG GTCGCGTTCA CCTCTTGCCA GATCTCTTAC AGCAAAGCCA TTCCTCCTCC 
ACTTCCTCCA CCTCCTCCTC CCCATCCTCC AGCCAGCCGA CACCCACCAT GTCCCCACAG 
ACACCCCAGG ACAAGCTCAC TCCTAACGAG ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA 
CACAAATCTT CCTCCTCCTT TACACCTTTT ATAGACCCCA GATTACTACA GATTTCTCCA 
TCTAGTGGGA CAACAGTGAC TTCTGTGGTG GGATTTTCCT GTGATGGAAT GAGATCGGAG 
GCCATAAGGC AAGATCCTAC CCGGAAGGGC TCAGTGGTCA ATGTGAATCC CACCAACACT 
AGGCCACAGA GCGACACCCC AGAGATTCGC AAATATAAGA AGAGGTTTAA CTCCGAGATT 
CTGTGCGCTG CCTTGTGGGG AGTGAACTTG TTGGTGGGTA CAGAGAGTGG CCTGATGCTG 
CTGGACAGAA GTGGCCAAGG GAAGGTGTAT CCTCTGATCA ACAGAAGACG ATTTCAACAG 
ATGGACGTCC TGGAAGGCTT GAATGTCTTG GTGACAATAT CTGGCAAAAA GGATAAGTTA 
CGTGTCTACT ATTTGTCCTG GTTAAGAAAT AAAATACTTC ACAACGATCC GGAAGTTGAG 
AAGAAGCAGG GTTGGACGAC CGTGGGAGAT TTGGAAGGAT GCGTCCACTA TAAAGTCGTA 
AAATATGAAA GAATCAAGTT TCTGGTCATT GCTTTGAAGA GTTCTGTGGA AGTGTATGCG 
TGGGCACCCA AGCCATATCA CAAATTCATG GCCTTTAAGT CGTTTGGAGA TTTGGTGCAT 
AAGCCATTAC TGGTGGATCT CACTGTTGAG GAAGGCCAGA GGTTGAAAGT GATCTATGGA 
TCCTGTGCTG GATTCCATGC TGTTGACGTG GATTCAGGAT CAGTCTATGA CATTTATCTA 
CCAACACACG TAAGAAAGAA CCCACACTCT ATGATCCAGT GCAGCATCAA ACCCCACGCA 
ATCATCATCC TCCCCAACAC GGATGGAATG GAGCTTCTGG TGTGCTATGA AGACGAAGGG 
GTCTATGTGA ACACCTATGG AAGGATTACC AAGGATGTGG TTCTGCAGTG GGGAGAGATG 
CCAACGTCTG TAGCGTATAT TCGATCCAAT CAGACGATGG GCTGGGGAGA AAAGGCCATA 
GAGATCCGGT CTGTGGAGAC GGGCCACTTG GACGGTGTGT TTATGCACAA AAGGGCTCAA 
AGACTGAAAT TCTTATGTGA ACGCAACGAC AAGGTGTTCT TTGCCTCGGT CCGGTCCGGG 
GGCAGCAGCC AGGTGTACTT CATGACGCTG GGCAGGACCT CTCTTCTGAG CTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_019931218.1 ) cDNA ORF clone, Tursiops truncatus(common bottlenose dolphin) -> XP_019786777.1 Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTu39177
Clone ID Related Accession (Same CDS sequence)	XM_019931218.1
Accession Version	XM_019931218.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Tursiops truncatus(common bottlenose dolphin)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017842906.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tursiops truncatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
CCGGCTGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
AAGGGTCGAC ACGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA CGTTACTGAG 
GATGAAGAGG AGGAAATCAA GCTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGCGGGGCC GGGTCCATCA CAGACCTCGT GAAGAACACC 
AAGGGCAACA CGCTCAAGGA GGACTGGATA GCCTACATCT CCAGGGAGAT CCTGAGGGGA 
CTGGCGCATC TCCACATCCA TCACGTGATT CACCGGGACA TCAAGGGCCA GAATGTATTG 
CTGACCGAGA ACGCAGAGGT GAAGCTTGTG GATTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACCGTGGGGA GGAGAAACAC CTTCATAGGC ACCCCGTACT GGATGGCTCC CGAGGTCATC 
GCCTGTGACG AGAACCCCGA CGCCACCTAT GATTACAGGA GTGACCTGTG GTCCTGCGGC 
ATCACAGCCA TTGAGATGGC AGAAGGCGCT CCCCCTCTCT GTGACATGCA TCCCATGAGA 
GCACTGTTTC TCATCCCCAG AAACCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCAAAA 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACA 
GAGCAGCTTC TGAAACATCC TTTTATCAGG GACCAGCCGA ATGAAAGGCA GGTTAGGATC 
CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACCGAG 
TACGAGTACA GCGGGAGTGA GGAGGAGGAG GAGGAGGCGC CGGAGCAGGA GGGGGAGCCC 
AGCTCCATCG TCAACGTGCC TGGCGAGTCC ACGCTGCGCC GGGACTTCCT GAGGCTGCAG 
CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGCGGCAGC AGCTCCTGCA GGAGCAGCAG 
CTCAGGGAGC AGGAAGAATA CAAGAGGCAG CTGCTGGCGG AGAGACAGAA GCGGATCGAG 
CAGCAGAAGG AGCAGCGGCG ACGGCTGGAA GAGCAACAAA GGAGAGAGCG GGAGGCCAGG 
AGGCAGCAGG AGCGCGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
CTGGAGAGAC GGCGGCAGGA GGAAGAGGAG CGCAGGCGGG CGGAGGAGGA CAAGAGGAGA 
GTCGACAGGG AGCAGGAGTA TATCAGGCGG CAGCTGGAAG AGGAGCAGCG GCACCTGGAA 
CTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
CTGGAGGAGC AGCGGCAGAC GGAGCGGCTC CAGAGGCAGC TGCAGCAGGA GCAAGCCTAT 
CTCCTGTCTC TGCAGCATGA GCCCCGGAGG CCGCCCCCGC CGCCGGACAG GAGCAAGGCC 
GGCCTGCACG GCGCCGAGCC CAAGGCCCAG CACGAGCCCG CCGACCGGGC CCGCGAGGTG 
GAAGATAGAT TTAGGAAAAC TAACCACAGC TCCCCTGAAG CCCAGTCTAA GCAGACAGGC 
AGAGTATTGG AGCCACCAGT GCCTTCCAGA TCAGAATCTT TTTCCAATGG CAACTCCGAG 
TCTGTGCACC CTGCCCTGCA GAGACCAGCC GAGCCACAGG TACAGTGGTC CCACCTGGCA 
TCTCTCAAGA ACAGTGTTTC CCCTGTCTCG CGATCCCATT CCTTCAGTGA CCCTTCTCCT 
CCCAAATTTG CACACCACCA TCTTCGCTCT CAGGACCCAT GTCCACCTTC CCGCAGTGAG 
GTGCTAAGTC AGAGTTCTGA CTCTAAGTCG GAGGTACCCG ACCCCGCCCA AAAGGCTTGG 
TCTAGATCAG ACAGTGACGA GGTCCCTCCA AGGGTTCCTG TGAGAACGAC GTCCCGGTCC 
CCCGTCCTGT CCCGTCGGGA TTCCCCACTG CAGGGCAGTG GGCAGCAAAA CAGCCAAGCA 
GGTCAGAGAA ACTCCACCAG CAGCATCGAG CCCAGGCTGC TATGGGAGAG AGTGGAGAAG 
CTGGTGCCCA GGCCTGGCAG TGGCAGCTCC TCGGGGTCCA GCAACTCAGG ATCCCAGCCC 
GGCTCCCACC CTGGGTCTCA GAGCGGCTCC GGGGAGCGCT TCAGAGTGAG ATCGTCATCC 
AAATCTGAGG GCTCGCCATC TCAGCGCCTT GAAAATGCAG CAAAAAAGCC TGAAGATAAG 
AAGGAAGTTT TTAGACCTCT CAAGCCCGCT GGCGAAGTGG ACCTGACGGC CCTGGCCAGA 
GAGCTCCGAG CGGCGGAAGA CGTGCGGCCG CCGCACAAGG TGACAGACTA CTCGTCGTCC 
AGCGAGGAGT CCGGGACAAC GGATGAGGAG GACGACGACG TGGAGCAGGA AGGCCCCGAG 
GAGCCCACCT CGGGGCCGGA GGACGCCCGA GCCGCGTCAT CTCTGAACTT GAGCAACGGT 
GAGACGGAGT CCGTGAAAAC GATGATCGTC CACGACGACG TGGAGAGTGA ACCAGCCATG 
ACCCCGTCCA AGGAGGGCAC CCTGATTGTC CGCCAGAGTG CAGTTGACCA AAAGCGCGCC 
AGCCATCATG AGAGCAATGG CTTTGCCGGT CGCGTTCACC TCTTGCCAGA TCTCTTACAG 
CAAAGCCATT CCTCCTCCAC TTCCTCCACC TCCTCCTCCC CATCCTCCAG CCAGCCGACA 
CCCACCATGT CCCCACAGAC ACCCCAGGAC AAGCTCACTC CTAACGAGAC TCAGTCCGCT 
AGTAGCACAC TCCAGAAACA CAAATCTTCC TCCTCCTTTA CACCTTTTAT AGACCCCAGA 
TTACTACAGA TTTCTCCATC TAGTGGGACA ACAGTGACTT CTGTGGTGGG ATTTTCCTGT 
GATGGAATGA GATCGGAGGC CATAAGGCAA GATCCTACCC GGAAGGGCTC AGTGGTCAAT 
GTGAATCCCA CCAACACTAG GCCACAGAGC GACACCCCAG AGATTCGCAA ATATAAGAAG 
AGGTTTAACT CCGAGATTCT GTGCGCTGCC TTGTGGGGAG TGAACTTGTT GGTGGGTACA 
GAGAGTGGCC TGATGCTGCT GGACAGAAGT GGCCAAGGGA AGGTGTATCC TCTGATCAAC 
AGAAGACGAT TTCAACAGAT GGACGTCCTG GAAGGCTTGA ATGTCTTGGT GACAATATCT 
GGCAAAAAGG ATAAGTTACG TGTCTACTAT TTGTCCTGGT TAAGAAATAA AATACTTCAC 
AACGATCCGG AAGTTGAGAA GAAGCAGGGT TGGACGACCG TGGGAGATTT GGAAGGATGC 
GTCCACTATA AAGTCGTAAA ATATGAAAGA ATCAAGTTTC TGGTCATTGC TTTGAAGAGT 
TCTGTGGAAG TGTATGCGTG GGCACCCAAG CCATATCACA AATTCATGGC CTTTAAGTCG 
TTTGGAGATT TGGTGCATAA GCCATTACTG GTGGATCTCA CTGTTGAGGA AGGCCAGAGG 
TTGAAAGTGA TCTATGGATC CTGTGCTGGA TTCCATGCTG TTGACGTGGA TTCAGGATCA 
GTCTATGACA TTTATCTACC AACACACATC CAGTGCAGCA TCAAACCCCA CGCAATCATC 
ATCCTCCCCA ACACGGATGG AATGGAGCTT CTGGTGTGCT ATGAAGACGA AGGGGTCTAT 
GTGAACACCT ATGGAAGGAT TACCAAGGAT GTGGTTCTGC AGTGGGGAGA GATGCCAACG 
TCTGTAGCGT ATATTCGATC CAATCAGACG ATGGGCTGGG GAGAAAAGGC CATAGAGATC 
CGGTCTGTGG AGACGGGCCA CTTGGACGGT GTGTTTATGC ACAAAAGGGC TCAAAGACTG 
AAATTCTTAT GTGAACGCAA CGACAAGGTG TTCTTTGCCT CGGTCCGGTC CGGGGGCAGC 
AGCCAGGTGT ACTTCATGAC GCTGGGCAGG ACCTCTCTTC TGAGCTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019786777.1
CDS	149..4279
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEAPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRQEEEERRRAEEDKRRVDREQEYIRRQLEEEQRHLELLQQQLLQEQAMLLECRWRELEEQRQTERLQRQLQQEQAYLLSLQHEPRRPPPPPDRSKAGLHGAEPKAQHEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVQWSHLASLKNSVSPVSRSHSFSDPSPPKFAHHHLRSQDPCPPSRSEVLSQSSDSKSEVPDPAQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPAGEVDLTALARELRAAEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGPEEPTSGPEDARAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSAVDQKRASHHESNGFAGRVHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTPNETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRSEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGDLVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_019931218.1
Organism	Tursiops truncatus(common bottlenose dolphin)
Definition	Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019931218.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
CCGGCTGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
AAGGGTCGAC ACGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA CGTTACTGAG 
GATGAAGAGG AGGAAATCAA GCTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGCGGGGCC GGGTCCATCA CAGACCTCGT GAAGAACACC 
AAGGGCAACA CGCTCAAGGA GGACTGGATA GCCTACATCT CCAGGGAGAT CCTGAGGGGA 
CTGGCGCATC TCCACATCCA TCACGTGATT CACCGGGACA TCAAGGGCCA GAATGTATTG 
CTGACCGAGA ACGCAGAGGT GAAGCTTGTG GATTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACCGTGGGGA GGAGAAACAC CTTCATAGGC ACCCCGTACT GGATGGCTCC CGAGGTCATC 
GCCTGTGACG AGAACCCCGA CGCCACCTAT GATTACAGGA GTGACCTGTG GTCCTGCGGC 
ATCACAGCCA TTGAGATGGC AGAAGGCGCT CCCCCTCTCT GTGACATGCA TCCCATGAGA 
GCACTGTTTC TCATCCCCAG AAACCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCAAAA 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACA 
GAGCAGCTTC TGAAACATCC TTTTATCAGG GACCAGCCGA ATGAAAGGCA GGTTAGGATC 
CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACCGAG 
TACGAGTACA GCGGGAGTGA GGAGGAGGAG GAGGAGGCGC CGGAGCAGGA GGGGGAGCCC 
AGCTCCATCG TCAACGTGCC TGGCGAGTCC ACGCTGCGCC GGGACTTCCT GAGGCTGCAG 
CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGCGGCAGC AGCTCCTGCA GGAGCAGCAG 
CTCAGGGAGC AGGAAGAATA CAAGAGGCAG CTGCTGGCGG AGAGACAGAA GCGGATCGAG 
CAGCAGAAGG AGCAGCGGCG ACGGCTGGAA GAGCAACAAA GGAGAGAGCG GGAGGCCAGG 
AGGCAGCAGG AGCGCGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
CTGGAGAGAC GGCGGCAGGA GGAAGAGGAG CGCAGGCGGG CGGAGGAGGA CAAGAGGAGA 
GTCGACAGGG AGCAGGAGTA TATCAGGCGG CAGCTGGAAG AGGAGCAGCG GCACCTGGAA 
CTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
CTGGAGGAGC AGCGGCAGAC GGAGCGGCTC CAGAGGCAGC TGCAGCAGGA GCAAGCCTAT 
CTCCTGTCTC TGCAGCATGA GCCCCGGAGG CCGCCCCCGC CGCCGGACAG GAGCAAGGCC 
GGCCTGCACG GCGCCGAGCC CAAGGCCCAG CACGAGCCCG CCGACCGGGC CCGCGAGGTG 
GAAGATAGAT TTAGGAAAAC TAACCACAGC TCCCCTGAAG CCCAGTCTAA GCAGACAGGC 
AGAGTATTGG AGCCACCAGT GCCTTCCAGA TCAGAATCTT TTTCCAATGG CAACTCCGAG 
TCTGTGCACC CTGCCCTGCA GAGACCAGCC GAGCCACAGG TACAGTGGTC CCACCTGGCA 
TCTCTCAAGA ACAGTGTTTC CCCTGTCTCG CGATCCCATT CCTTCAGTGA CCCTTCTCCT 
CCCAAATTTG CACACCACCA TCTTCGCTCT CAGGACCCAT GTCCACCTTC CCGCAGTGAG 
GTGCTAAGTC AGAGTTCTGA CTCTAAGTCG GAGGTACCCG ACCCCGCCCA AAAGGCTTGG 
TCTAGATCAG ACAGTGACGA GGTCCCTCCA AGGGTTCCTG TGAGAACGAC GTCCCGGTCC 
CCCGTCCTGT CCCGTCGGGA TTCCCCACTG CAGGGCAGTG GGCAGCAAAA CAGCCAAGCA 
GGTCAGAGAA ACTCCACCAG CAGCATCGAG CCCAGGCTGC TATGGGAGAG AGTGGAGAAG 
CTGGTGCCCA GGCCTGGCAG TGGCAGCTCC TCGGGGTCCA GCAACTCAGG ATCCCAGCCC 
GGCTCCCACC CTGGGTCTCA GAGCGGCTCC GGGGAGCGCT TCAGAGTGAG ATCGTCATCC 
AAATCTGAGG GCTCGCCATC TCAGCGCCTT GAAAATGCAG CAAAAAAGCC TGAAGATAAG 
AAGGAAGTTT TTAGACCTCT CAAGCCCGCT GGCGAAGTGG ACCTGACGGC CCTGGCCAGA 
GAGCTCCGAG CGGCGGAAGA CGTGCGGCCG CCGCACAAGG TGACAGACTA CTCGTCGTCC 
AGCGAGGAGT CCGGGACAAC GGATGAGGAG GACGACGACG TGGAGCAGGA AGGCCCCGAG 
GAGCCCACCT CGGGGCCGGA GGACGCCCGA GCCGCGTCAT CTCTGAACTT GAGCAACGGT 
GAGACGGAGT CCGTGAAAAC GATGATCGTC CACGACGACG TGGAGAGTGA ACCAGCCATG 
ACCCCGTCCA AGGAGGGCAC CCTGATTGTC CGCCAGAGTG CAGTTGACCA AAAGCGCGCC 
AGCCATCATG AGAGCAATGG CTTTGCCGGT CGCGTTCACC TCTTGCCAGA TCTCTTACAG 
CAAAGCCATT CCTCCTCCAC TTCCTCCACC TCCTCCTCCC CATCCTCCAG CCAGCCGACA 
CCCACCATGT CCCCACAGAC ACCCCAGGAC AAGCTCACTC CTAACGAGAC TCAGTCCGCT 
AGTAGCACAC TCCAGAAACA CAAATCTTCC TCCTCCTTTA CACCTTTTAT AGACCCCAGA 
TTACTACAGA TTTCTCCATC TAGTGGGACA ACAGTGACTT CTGTGGTGGG ATTTTCCTGT 
GATGGAATGA GATCGGAGGC CATAAGGCAA GATCCTACCC GGAAGGGCTC AGTGGTCAAT 
GTGAATCCCA CCAACACTAG GCCACAGAGC GACACCCCAG AGATTCGCAA ATATAAGAAG 
AGGTTTAACT CCGAGATTCT GTGCGCTGCC TTGTGGGGAG TGAACTTGTT GGTGGGTACA 
GAGAGTGGCC TGATGCTGCT GGACAGAAGT GGCCAAGGGA AGGTGTATCC TCTGATCAAC 
AGAAGACGAT TTCAACAGAT GGACGTCCTG GAAGGCTTGA ATGTCTTGGT GACAATATCT 
GGCAAAAAGG ATAAGTTACG TGTCTACTAT TTGTCCTGGT TAAGAAATAA AATACTTCAC 
AACGATCCGG AAGTTGAGAA GAAGCAGGGT TGGACGACCG TGGGAGATTT GGAAGGATGC 
GTCCACTATA AAGTCGTAAA ATATGAAAGA ATCAAGTTTC TGGTCATTGC TTTGAAGAGT 
TCTGTGGAAG TGTATGCGTG GGCACCCAAG CCATATCACA AATTCATGGC CTTTAAGTCG 
TTTGGAGATT TGGTGCATAA GCCATTACTG GTGGATCTCA CTGTTGAGGA AGGCCAGAGG 
TTGAAAGTGA TCTATGGATC CTGTGCTGGA TTCCATGCTG TTGACGTGGA TTCAGGATCA 
GTCTATGACA TTTATCTACC AACACACATC CAGTGCAGCA TCAAACCCCA CGCAATCATC 
ATCCTCCCCA ACACGGATGG AATGGAGCTT CTGGTGTGCT ATGAAGACGA AGGGGTCTAT 
GTGAACACCT ATGGAAGGAT TACCAAGGAT GTGGTTCTGC AGTGGGGAGA GATGCCAACG 
TCTGTAGCGT ATATTCGATC CAATCAGACG ATGGGCTGGG GAGAAAAGGC CATAGAGATC 
CGGTCTGTGG AGACGGGCCA CTTGGACGGT GTGTTTATGC ACAAAAGGGC TCAAAGACTG 
AAATTCTTAT GTGAACGCAA CGACAAGGTG TTCTTTGCCT CGGTCCGGTC CGGGGGCAGC 
AGCCAGGTGT ACTTCATGAC GCTGGGCAGG ACCTCTCTTC TGAGCTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_019931219.1 ) cDNA ORF clone, Tursiops truncatus(common bottlenose dolphin) -> XP_019786778.1 Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X2, mRNA.

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CloneID	OTu39178
Clone ID Related Accession (Same CDS sequence)	XM_019931219.1
Accession Version	XM_019931219.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Tursiops truncatus(common bottlenose dolphin)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017842906.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tursiops truncatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
CCGGCTGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
AAGGGTCGAC ACGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA CGTTACTGAG 
GATGAAGAGG AGGAAATCAA GCTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGCGGGGCC GGGTCCATCA CAGACCTCGT GAAGAACACC 
AAGGGCAACA CGCTCAAGGA GGACTGGATA GCCTACATCT CCAGGGAGAT CCTGAGGGGA 
CTGGCGCATC TCCACATCCA TCACGTGATT CACCGGGACA TCAAGGGCCA GAATGTATTG 
CTGACCGAGA ACGCAGAGGT GAAGCTTGTG GATTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACCGTGGGGA GGAGAAACAC CTTCATAGGC ACCCCGTACT GGATGGCTCC CGAGGTCATC 
GCCTGTGACG AGAACCCCGA CGCCACCTAT GATTACAGGA GTGACCTGTG GTCCTGCGGC 
ATCACAGCCA TTGAGATGGC AGAAGGCGCT CCCCCTCTCT GTGACATGCA TCCCATGAGA 
GCACTGTTTC TCATCCCCAG AAACCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCAAAA 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACA 
GAGCAGCTTC TGAAACATCC TTTTATCAGG GACCAGCCGA ATGAAAGGCA GGTTAGGATC 
CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACCGAG 
TACGAGTACA GCGGGAGTGA GGAGGAGGAG GAGGAGGCGC CGGAGCAGGA GGGGGAGCCC 
AGCTCCATCG TCAACGTGCC TGGCGAGTCC ACGCTGCGCC GGGACTTCCT GAGGCTGCAG 
CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGCGGCAGC AGCTCCTGCA GGAGCAGCAG 
CTCAGGGAGC AGGAAGAATA CAAGAGGCAG CTGCTGGCGG AGAGACAGAA GCGGATCGAG 
CAGCAGAAGG AGCAGCGGCG ACGGCTGGAA GAGCAACAAA GGAGAGAGCG GGAGGCCAGG 
AGGCAGCAGG AGCGCGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
CTGGAGAGAC GGCGGCAGGA GGAAGAGGAG CGCAGGCGGG CGGAGGAGGA CAAGAGGAGA 
GTCGACAGGG AGCAGGAGTA TATCAGGCGG CAGCTGGAAG AGGAGCAGCG GCACCTGGAA 
CTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
CTGGAGGAGC AGCGGCAGAC GGAGCGGCTC CAGAGGCAGC TGCAGCAGGA GCAAGCCTAT 
CTCCTGTCTC TGCAGCATGA GCCCCGGAGG CCGCCCCCGC CGCCGGACAG GAGCAAGGCC 
GGCCTGCACG GCGCCGAGCC CAAGGCCCAG CACGAGCCCG CCGACCGGGC CCGCGAGGTA 
CAGTGGTCCC ACCTGGCATC TCTCAAGAAC AGTGTTTCCC CTGTCTCGCG ATCCCATTCC 
TTCAGTGACC CTTCTCCTCC CAAATTTGCA CACCACCATC TTCGCTCTCA GGACCCATGT 
CCACCTTCCC GCAGTGAGGT GCTAAGTCAG AGTTCTGACT CTAAGTCGGA GGTACCCGAC 
CCCGCCCAAA AGGCTTGGTC TAGATCAGAC AGTGACGAGG TCCCTCCAAG GGTTCCTGTG 
AGAACGACGT CCCGGTCCCC CGTCCTGTCC CGTCGGGATT CCCCACTGCA GGGCAGTGGG 
CAGCAAAACA GCCAAGCAGG TCAGAGAAAC TCCACCAGCA GCATCGAGCC CAGGCTGCTA 
TGGGAGAGAG TGGAGAAGCT GGTGCCCAGG CCTGGCAGTG GCAGCTCCTC GGGGTCCAGC 
AACTCAGGAT CCCAGCCCGG CTCCCACCCT GGGTCTCAGA GCGGCTCCGG GGAGCGCTTC 
AGAGTGAGAT CGTCATCCAA ATCTGAGGGC TCGCCATCTC AGCGCCTTGA AAATGCAGCA 
AAAAAGCCTG AAGATAAGAA GGAAGTTTTT AGACCTCTCA AGCCCGCTGG CGAAGTGGAC 
CTGACGGCCC TGGCCAGAGA GCTCCGAGCG GCGGAAGACG TGCGGCCGCC GCACAAGGTG 
ACAGACTACT CGTCGTCCAG CGAGGAGTCC GGGACAACGG ATGAGGAGGA CGACGACGTG 
GAGCAGGAAG GCCCCGAGGA GCCCACCTCG GGGCCGGAGG ACGCCCGAGC CGCGTCATCT 
CTGAACTTGA GCAACGGTGA GACGGAGTCC GTGAAAACGA TGATCGTCCA CGACGACGTG 
GAGAGTGAAC CAGCCATGAC CCCGTCCAAG GAGGGCACCC TGATTGTCCG CCAGAGTGCA 
GTTGACCAAA AGCGCGCCAG CCATCATGAG AGCAATGGCT TTGCCGGTCG CGTTCACCTC 
TTGCCAGATC TCTTACAGCA AAGCCATTCC TCCTCCACTT CCTCCACCTC CTCCTCCCCA 
TCCTCCAGCC AGCCGACACC CACCATGTCC CCACAGACAC CCCAGGACAA GCTCACTCCT 
AACGAGACTC AGTCCGCTAG TAGCACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA 
CCTTTTATAG ACCCCAGATT ACTACAGATT TCTCCATCTA GTGGGACAAC AGTGACTTCT 
GTGGTGGGAT TTTCCTGTGA TGGAATGAGA TCGGAGGCCA TAAGGCAAGA TCCTACCCGG 
AAGGGCTCAG TGGTCAATGT GAATCCCACC AACACTAGGC CACAGAGCGA CACCCCAGAG 
ATTCGCAAAT ATAAGAAGAG GTTTAACTCC GAGATTCTGT GCGCTGCCTT GTGGGGAGTG 
AACTTGTTGG TGGGTACAGA GAGTGGCCTG ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG 
GTGTATCCTC TGATCAACAG AAGACGATTT CAACAGATGG ACGTCCTGGA AGGCTTGAAT 
GTCTTGGTGA CAATATCTGG CAAAAAGGAT AAGTTACGTG TCTACTATTT GTCCTGGTTA 
AGAAATAAAA TACTTCACAA CGATCCGGAA GTTGAGAAGA AGCAGGGTTG GACGACCGTG 
GGAGATTTGG AAGGATGCGT CCACTATAAA GTCGTAAAAT ATGAAAGAAT CAAGTTTCTG 
GTCATTGCTT TGAAGAGTTC TGTGGAAGTG TATGCGTGGG CACCCAAGCC ATATCACAAA 
TTCATGGCCT TTAAGTCGTT TGGAGATTTG GTGCATAAGC CATTACTGGT GGATCTCACT 
GTTGAGGAAG GCCAGAGGTT GAAAGTGATC TATGGATCCT GTGCTGGATT CCATGCTGTT 
GACGTGGATT CAGGATCAGT CTATGACATT TATCTACCAA CACACGTAAG AAAGAACCCA 
CACTCTATGA TCCAGTGCAG CATCAAACCC CACGCAATCA TCATCCTCCC CAACACGGAT 
GGAATGGAGC TTCTGGTGTG CTATGAAGAC GAAGGGGTCT ATGTGAACAC CTATGGAAGG 
ATTACCAAGG ATGTGGTTCT GCAGTGGGGA GAGATGCCAA CGTCTGTAGC GTATATTCGA 
TCCAATCAGA CGATGGGCTG GGGAGAAAAG GCCATAGAGA TCCGGTCTGT GGAGACGGGC 
CACTTGGACG GTGTGTTTAT GCACAAAAGG GCTCAAAGAC TGAAATTCTT ATGTGAACGC 
AACGACAAGG TGTTCTTTGC CTCGGTCCGG TCCGGGGGCA GCAGCCAGGT GTACTTCATG 
ACGCTGGGCA GGACCTCTCT TCTGAGCTGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019786778.1
CDS	149..4141
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEAPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRQEEEERRRAEEDKRRVDREQEYIRRQLEEEQRHLELLQQQLLQEQAMLLECRWRELEEQRQTERLQRQLQQEQAYLLSLQHEPRRPPPPPDRSKAGLHGAEPKAQHEPADRAREVQWSHLASLKNSVSPVSRSHSFSDPSPPKFAHHHLRSQDPCPPSRSEVLSQSSDSKSEVPDPAQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPAGEVDLTALARELRAAEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGPEEPTSGPEDARAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSAVDQKRASHHESNGFAGRVHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTPNETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRSEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGDLVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_019931219.1
Organism	Tursiops truncatus(common bottlenose dolphin)
Definition	Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019931219.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
CCGGCTGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
AAGGGTCGAC ACGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA CGTTACTGAG 
GATGAAGAGG AGGAAATCAA GCTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGCGGGGCC GGGTCCATCA CAGACCTCGT GAAGAACACC 
AAGGGCAACA CGCTCAAGGA GGACTGGATA GCCTACATCT CCAGGGAGAT CCTGAGGGGA 
CTGGCGCATC TCCACATCCA TCACGTGATT CACCGGGACA TCAAGGGCCA GAATGTATTG 
CTGACCGAGA ACGCAGAGGT GAAGCTTGTG GATTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACCGTGGGGA GGAGAAACAC CTTCATAGGC ACCCCGTACT GGATGGCTCC CGAGGTCATC 
GCCTGTGACG AGAACCCCGA CGCCACCTAT GATTACAGGA GTGACCTGTG GTCCTGCGGC 
ATCACAGCCA TTGAGATGGC AGAAGGCGCT CCCCCTCTCT GTGACATGCA TCCCATGAGA 
GCACTGTTTC TCATCCCCAG AAACCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCAAAA 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACA 
GAGCAGCTTC TGAAACATCC TTTTATCAGG GACCAGCCGA ATGAAAGGCA GGTTAGGATC 
CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACCGAG 
TACGAGTACA GCGGGAGTGA GGAGGAGGAG GAGGAGGCGC CGGAGCAGGA GGGGGAGCCC 
AGCTCCATCG TCAACGTGCC TGGCGAGTCC ACGCTGCGCC GGGACTTCCT GAGGCTGCAG 
CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGCGGCAGC AGCTCCTGCA GGAGCAGCAG 
CTCAGGGAGC AGGAAGAATA CAAGAGGCAG CTGCTGGCGG AGAGACAGAA GCGGATCGAG 
CAGCAGAAGG AGCAGCGGCG ACGGCTGGAA GAGCAACAAA GGAGAGAGCG GGAGGCCAGG 
AGGCAGCAGG AGCGCGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
CTGGAGAGAC GGCGGCAGGA GGAAGAGGAG CGCAGGCGGG CGGAGGAGGA CAAGAGGAGA 
GTCGACAGGG AGCAGGAGTA TATCAGGCGG CAGCTGGAAG AGGAGCAGCG GCACCTGGAA 
CTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
CTGGAGGAGC AGCGGCAGAC GGAGCGGCTC CAGAGGCAGC TGCAGCAGGA GCAAGCCTAT 
CTCCTGTCTC TGCAGCATGA GCCCCGGAGG CCGCCCCCGC CGCCGGACAG GAGCAAGGCC 
GGCCTGCACG GCGCCGAGCC CAAGGCCCAG CACGAGCCCG CCGACCGGGC CCGCGAGGTA 
CAGTGGTCCC ACCTGGCATC TCTCAAGAAC AGTGTTTCCC CTGTCTCGCG ATCCCATTCC 
TTCAGTGACC CTTCTCCTCC CAAATTTGCA CACCACCATC TTCGCTCTCA GGACCCATGT 
CCACCTTCCC GCAGTGAGGT GCTAAGTCAG AGTTCTGACT CTAAGTCGGA GGTACCCGAC 
CCCGCCCAAA AGGCTTGGTC TAGATCAGAC AGTGACGAGG TCCCTCCAAG GGTTCCTGTG 
AGAACGACGT CCCGGTCCCC CGTCCTGTCC CGTCGGGATT CCCCACTGCA GGGCAGTGGG 
CAGCAAAACA GCCAAGCAGG TCAGAGAAAC TCCACCAGCA GCATCGAGCC CAGGCTGCTA 
TGGGAGAGAG TGGAGAAGCT GGTGCCCAGG CCTGGCAGTG GCAGCTCCTC GGGGTCCAGC 
AACTCAGGAT CCCAGCCCGG CTCCCACCCT GGGTCTCAGA GCGGCTCCGG GGAGCGCTTC 
AGAGTGAGAT CGTCATCCAA ATCTGAGGGC TCGCCATCTC AGCGCCTTGA AAATGCAGCA 
AAAAAGCCTG AAGATAAGAA GGAAGTTTTT AGACCTCTCA AGCCCGCTGG CGAAGTGGAC 
CTGACGGCCC TGGCCAGAGA GCTCCGAGCG GCGGAAGACG TGCGGCCGCC GCACAAGGTG 
ACAGACTACT CGTCGTCCAG CGAGGAGTCC GGGACAACGG ATGAGGAGGA CGACGACGTG 
GAGCAGGAAG GCCCCGAGGA GCCCACCTCG GGGCCGGAGG ACGCCCGAGC CGCGTCATCT 
CTGAACTTGA GCAACGGTGA GACGGAGTCC GTGAAAACGA TGATCGTCCA CGACGACGTG 
GAGAGTGAAC CAGCCATGAC CCCGTCCAAG GAGGGCACCC TGATTGTCCG CCAGAGTGCA 
GTTGACCAAA AGCGCGCCAG CCATCATGAG AGCAATGGCT TTGCCGGTCG CGTTCACCTC 
TTGCCAGATC TCTTACAGCA AAGCCATTCC TCCTCCACTT CCTCCACCTC CTCCTCCCCA 
TCCTCCAGCC AGCCGACACC CACCATGTCC CCACAGACAC CCCAGGACAA GCTCACTCCT 
AACGAGACTC AGTCCGCTAG TAGCACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA 
CCTTTTATAG ACCCCAGATT ACTACAGATT TCTCCATCTA GTGGGACAAC AGTGACTTCT 
GTGGTGGGAT TTTCCTGTGA TGGAATGAGA TCGGAGGCCA TAAGGCAAGA TCCTACCCGG 
AAGGGCTCAG TGGTCAATGT GAATCCCACC AACACTAGGC CACAGAGCGA CACCCCAGAG 
ATTCGCAAAT ATAAGAAGAG GTTTAACTCC GAGATTCTGT GCGCTGCCTT GTGGGGAGTG 
AACTTGTTGG TGGGTACAGA GAGTGGCCTG ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG 
GTGTATCCTC TGATCAACAG AAGACGATTT CAACAGATGG ACGTCCTGGA AGGCTTGAAT 
GTCTTGGTGA CAATATCTGG CAAAAAGGAT AAGTTACGTG TCTACTATTT GTCCTGGTTA 
AGAAATAAAA TACTTCACAA CGATCCGGAA GTTGAGAAGA AGCAGGGTTG GACGACCGTG 
GGAGATTTGG AAGGATGCGT CCACTATAAA GTCGTAAAAT ATGAAAGAAT CAAGTTTCTG 
GTCATTGCTT TGAAGAGTTC TGTGGAAGTG TATGCGTGGG CACCCAAGCC ATATCACAAA 
TTCATGGCCT TTAAGTCGTT TGGAGATTTG GTGCATAAGC CATTACTGGT GGATCTCACT 
GTTGAGGAAG GCCAGAGGTT GAAAGTGATC TATGGATCCT GTGCTGGATT CCATGCTGTT 
GACGTGGATT CAGGATCAGT CTATGACATT TATCTACCAA CACACGTAAG AAAGAACCCA 
CACTCTATGA TCCAGTGCAG CATCAAACCC CACGCAATCA TCATCCTCCC CAACACGGAT 
GGAATGGAGC TTCTGGTGTG CTATGAAGAC GAAGGGGTCT ATGTGAACAC CTATGGAAGG 
ATTACCAAGG ATGTGGTTCT GCAGTGGGGA GAGATGCCAA CGTCTGTAGC GTATATTCGA 
TCCAATCAGA CGATGGGCTG GGGAGAAAAG GCCATAGAGA TCCGGTCTGT GGAGACGGGC 
CACTTGGACG GTGTGTTTAT GCACAAAAGG GCTCAAAGAC TGAAATTCTT ATGTGAACGC 
AACGACAAGG TGTTCTTTGC CTCGGTCCGG TCCGGGGGCA GCAGCCAGGT GTACTTCATG 
ACGCTGGGCA GGACCTCTCT TCTGAGCTGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_019931220.1 ) cDNA ORF clone, Tursiops truncatus(common bottlenose dolphin) -> XP_019786779.1 Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OTu39179
Clone ID Related Accession (Same CDS sequence)	XM_019931220.1
Accession Version	XM_019931220.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Tursiops truncatus(common bottlenose dolphin)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017842906.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tursiops truncatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
CCGGCTGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
AAGGGTCGAC ACGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA CGTTACTGAG 
GATGAAGAGG AGGAAATCAA GCTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGCGGGGCC GGGTCCATCA CAGACCTCGT GAAGAACACC 
AAGGGCAACA CGCTCAAGGA GGACTGGATA GCCTACATCT CCAGGGAGAT CCTGAGGGGA 
CTGGCGCATC TCCACATCCA TCACGTGATT CACCGGGACA TCAAGGGCCA GAATGTATTG 
CTGACCGAGA ACGCAGAGGT GAAGCTTGTG GATTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACCGTGGGGA GGAGAAACAC CTTCATAGGC ACCCCGTACT GGATGGCTCC CGAGGTCATC 
GCCTGTGACG AGAACCCCGA CGCCACCTAT GATTACAGGA GTGACCTGTG GTCCTGCGGC 
ATCACAGCCA TTGAGATGGC AGAAGGCGCT CCCCCTCTCT GTGACATGCA TCCCATGAGA 
GCACTGTTTC TCATCCCCAG AAACCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCAAAA 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACA 
GAGCAGCTTC TGAAACATCC TTTTATCAGG GACCAGCCGA ATGAAAGGCA GGTTAGGATC 
CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACCGAG 
TACGAGTACA GCGGGAGTGA GGAGGAGGAG GAGGAGGCGC CGGAGCAGGA GGGGGAGCCC 
AGCTCCATCG TCAACGTGCC TGGCGAGTCC ACGCTGCGCC GGGACTTCCT GAGGCTGCAG 
CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGCGGCAGC AGCTCCTGCA GGAGCAGCAG 
CTCAGGGAGC AGGAAGAATA CAAGAGGCAG CTGCTGGCGG AGAGACAGAA GCGGATCGAG 
CAGCAGAAGG AGCAGCGGCG ACGGCTGGAA GAGCAACAAA GGAGAGAGCG GGAGGCCAGG 
AGGCAGCAGG AGCGCGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
CTGGAGAGAC GGCGGCAGGA GGAAGAGGAG CGCAGGCGGG CGGAGGAGGA CAAGAGGAGA 
GTCGACAGGG AGCAGGAGTA TATCAGGCGG CAGCTGGAAG AGGAGCAGCG GCACCTGGAA 
CTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
CTGGAGGAGC AGCGGCAGAC GGAGCGGCTC CAGAGGCAGC TGCAGCAGGA GCAAGCCTAT 
CTCCTGTCTC TGCAGCATGA GCCCCGGAGG CCGCCCCCGC CGCCGGACAG GAGCAAGGCC 
GGCCTGCACG GCGCCGAGCC CAAGGCCCAG CACGAGCCCG CCGACCGGGC CCGCGAGTGG 
TCCCACCTGG CATCTCTCAA GAACAGTGTT TCCCCTGTCT CGCGATCCCA TTCCTTCAGT 
GACCCTTCTC CTCCCAAATT TGCACACCAC CATCTTCGCT CTCAGGACCC ATGTCCACCT 
TCCCGCAGTG AGGTGCTAAG TCAGAGTTCT GACTCTAAGT CGGAGGTACC CGACCCCGCC 
CAAAAGGCTT GGTCTAGATC AGACAGTGAC GAGGTCCCTC CAAGGGTTCC TGTGAGAACG 
ACGTCCCGGT CCCCCGTCCT GTCCCGTCGG GATTCCCCAC TGCAGGGCAG TGGGCAGCAA 
AACAGCCAAG CAGGTCAGAG AAACTCCACC AGCAGCATCG AGCCCAGGCT GCTATGGGAG 
AGAGTGGAGA AGCTGGTGCC CAGGCCTGGC AGTGGCAGCT CCTCGGGGTC CAGCAACTCA 
GGATCCCAGC CCGGCTCCCA CCCTGGGTCT CAGAGCGGCT CCGGGGAGCG CTTCAGAGTG 
AGATCGTCAT CCAAATCTGA GGGCTCGCCA TCTCAGCGCC TTGAAAATGC AGCAAAAAAG 
CCTGAAGATA AGAAGGAAGT TTTTAGACCT CTCAAGCCCG CTGGCGAAGT GGACCTGACG 
GCCCTGGCCA GAGAGCTCCG AGCGGCGGAA GACGTGCGGC CGCCGCACAA GGTGACAGAC 
TACTCGTCGT CCAGCGAGGA GTCCGGGACA ACGGATGAGG AGGACGACGA CGTGGAGCAG 
GAAGGCCCCG AGGAGCCCAC CTCGGGGCCG GAGGACGCCC GAGCCGCGTC ATCTCTGAAC 
TTGAGCAACG GTGAGACGGA GTCCGTGAAA ACGATGATCG TCCACGACGA CGTGGAGAGT 
GAACCAGCCA TGACCCCGTC CAAGGAGGGC ACCCTGATTG TCCGCCAGAG TGCAGTTGAC 
CAAAAGCGCG CCAGCCATCA TGAGAGCAAT GGCTTTGCCG GTCGCGTTCA CCTCTTGCCA 
GATCTCTTAC AGCAAAGCCA TTCCTCCTCC ACTTCCTCCA CCTCCTCCTC CCCATCCTCC 
AGCCAGCCGA CACCCACCAT GTCCCCACAG ACACCCCAGG ACAAGCTCAC TCCTAACGAG 
ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA CACAAATCTT CCTCCTCCTT TACACCTTTT 
ATAGACCCCA GATTACTACA GATTTCTCCA TCTAGTGGGA CAACAGTGAC TTCTGTGGTG 
GGATTTTCCT GTGATGGAAT GAGATCGGAG GCCATAAGGC AAGATCCTAC CCGGAAGGGC 
TCAGTGGTCA ATGTGAATCC CACCAACACT AGGCCACAGA GCGACACCCC AGAGATTCGC 
AAATATAAGA AGAGGTTTAA CTCCGAGATT CTGTGCGCTG CCTTGTGGGG AGTGAACTTG 
TTGGTGGGTA CAGAGAGTGG CCTGATGCTG CTGGACAGAA GTGGCCAAGG GAAGGTGTAT 
CCTCTGATCA ACAGAAGACG ATTTCAACAG ATGGACGTCC TGGAAGGCTT GAATGTCTTG 
GTGACAATAT CTGGCAAAAA GGATAAGTTA CGTGTCTACT ATTTGTCCTG GTTAAGAAAT 
AAAATACTTC ACAACGATCC GGAAGTTGAG AAGAAGCAGG GTTGGACGAC CGTGGGAGAT 
TTGGAAGGAT GCGTCCACTA TAAAGTCGTA AAATATGAAA GAATCAAGTT TCTGGTCATT 
GCTTTGAAGA GTTCTGTGGA AGTGTATGCG TGGGCACCCA AGCCATATCA CAAATTCATG 
GCCTTTAAGT CGTTTGGAGA TTTGGTGCAT AAGCCATTAC TGGTGGATCT CACTGTTGAG 
GAAGGCCAGA GGTTGAAAGT GATCTATGGA TCCTGTGCTG GATTCCATGC TGTTGACGTG 
GATTCAGGAT CAGTCTATGA CATTTATCTA CCAACACACG TAAGAAAGAA CCCACACTCT 
ATGATCCAGT GCAGCATCAA ACCCCACGCA ATCATCATCC TCCCCAACAC GGATGGAATG 
GAGCTTCTGG TGTGCTATGA AGACGAAGGG GTCTATGTGA ACACCTATGG AAGGATTACC 
AAGGATGTGG TTCTGCAGTG GGGAGAGATG CCAACGTCTG TAGCGTATAT TCGATCCAAT 
CAGACGATGG GCTGGGGAGA AAAGGCCATA GAGATCCGGT CTGTGGAGAC GGGCCACTTG 
GACGGTGTGT TTATGCACAA AAGGGCTCAA AGACTGAAAT TCTTATGTGA ACGCAACGAC 
AAGGTGTTCT TTGCCTCGGT CCGGTCCGGG GGCAGCAGCC AGGTGTACTT CATGACGCTG 
GGCAGGACCT CTCTTCTGAG CTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019786779.1
CDS	149..4135
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEAPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRQEEEERRRAEEDKRRVDREQEYIRRQLEEEQRHLELLQQQLLQEQAMLLECRWRELEEQRQTERLQRQLQQEQAYLLSLQHEPRRPPPPPDRSKAGLHGAEPKAQHEPADRAREWSHLASLKNSVSPVSRSHSFSDPSPPKFAHHHLRSQDPCPPSRSEVLSQSSDSKSEVPDPAQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPAGEVDLTALARELRAAEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGPEEPTSGPEDARAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSAVDQKRASHHESNGFAGRVHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTPNETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRSEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGDLVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_019931220.1
Organism	Tursiops truncatus(common bottlenose dolphin)
Definition	Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019931220.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
CCGGCTGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
AAGGGTCGAC ACGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA CGTTACTGAG 
GATGAAGAGG AGGAAATCAA GCTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGCGGGGCC GGGTCCATCA CAGACCTCGT GAAGAACACC 
AAGGGCAACA CGCTCAAGGA GGACTGGATA GCCTACATCT CCAGGGAGAT CCTGAGGGGA 
CTGGCGCATC TCCACATCCA TCACGTGATT CACCGGGACA TCAAGGGCCA GAATGTATTG 
CTGACCGAGA ACGCAGAGGT GAAGCTTGTG GATTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACCGTGGGGA GGAGAAACAC CTTCATAGGC ACCCCGTACT GGATGGCTCC CGAGGTCATC 
GCCTGTGACG AGAACCCCGA CGCCACCTAT GATTACAGGA GTGACCTGTG GTCCTGCGGC 
ATCACAGCCA TTGAGATGGC AGAAGGCGCT CCCCCTCTCT GTGACATGCA TCCCATGAGA 
GCACTGTTTC TCATCCCCAG AAACCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCAAAA 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACA 
GAGCAGCTTC TGAAACATCC TTTTATCAGG GACCAGCCGA ATGAAAGGCA GGTTAGGATC 
CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACCGAG 
TACGAGTACA GCGGGAGTGA GGAGGAGGAG GAGGAGGCGC CGGAGCAGGA GGGGGAGCCC 
AGCTCCATCG TCAACGTGCC TGGCGAGTCC ACGCTGCGCC GGGACTTCCT GAGGCTGCAG 
CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGCGGCAGC AGCTCCTGCA GGAGCAGCAG 
CTCAGGGAGC AGGAAGAATA CAAGAGGCAG CTGCTGGCGG AGAGACAGAA GCGGATCGAG 
CAGCAGAAGG AGCAGCGGCG ACGGCTGGAA GAGCAACAAA GGAGAGAGCG GGAGGCCAGG 
AGGCAGCAGG AGCGCGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
CTGGAGAGAC GGCGGCAGGA GGAAGAGGAG CGCAGGCGGG CGGAGGAGGA CAAGAGGAGA 
GTCGACAGGG AGCAGGAGTA TATCAGGCGG CAGCTGGAAG AGGAGCAGCG GCACCTGGAA 
CTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
CTGGAGGAGC AGCGGCAGAC GGAGCGGCTC CAGAGGCAGC TGCAGCAGGA GCAAGCCTAT 
CTCCTGTCTC TGCAGCATGA GCCCCGGAGG CCGCCCCCGC CGCCGGACAG GAGCAAGGCC 
GGCCTGCACG GCGCCGAGCC CAAGGCCCAG CACGAGCCCG CCGACCGGGC CCGCGAGTGG 
TCCCACCTGG CATCTCTCAA GAACAGTGTT TCCCCTGTCT CGCGATCCCA TTCCTTCAGT 
GACCCTTCTC CTCCCAAATT TGCACACCAC CATCTTCGCT CTCAGGACCC ATGTCCACCT 
TCCCGCAGTG AGGTGCTAAG TCAGAGTTCT GACTCTAAGT CGGAGGTACC CGACCCCGCC 
CAAAAGGCTT GGTCTAGATC AGACAGTGAC GAGGTCCCTC CAAGGGTTCC TGTGAGAACG 
ACGTCCCGGT CCCCCGTCCT GTCCCGTCGG GATTCCCCAC TGCAGGGCAG TGGGCAGCAA 
AACAGCCAAG CAGGTCAGAG AAACTCCACC AGCAGCATCG AGCCCAGGCT GCTATGGGAG 
AGAGTGGAGA AGCTGGTGCC CAGGCCTGGC AGTGGCAGCT CCTCGGGGTC CAGCAACTCA 
GGATCCCAGC CCGGCTCCCA CCCTGGGTCT CAGAGCGGCT CCGGGGAGCG CTTCAGAGTG 
AGATCGTCAT CCAAATCTGA GGGCTCGCCA TCTCAGCGCC TTGAAAATGC AGCAAAAAAG 
CCTGAAGATA AGAAGGAAGT TTTTAGACCT CTCAAGCCCG CTGGCGAAGT GGACCTGACG 
GCCCTGGCCA GAGAGCTCCG AGCGGCGGAA GACGTGCGGC CGCCGCACAA GGTGACAGAC 
TACTCGTCGT CCAGCGAGGA GTCCGGGACA ACGGATGAGG AGGACGACGA CGTGGAGCAG 
GAAGGCCCCG AGGAGCCCAC CTCGGGGCCG GAGGACGCCC GAGCCGCGTC ATCTCTGAAC 
TTGAGCAACG GTGAGACGGA GTCCGTGAAA ACGATGATCG TCCACGACGA CGTGGAGAGT 
GAACCAGCCA TGACCCCGTC CAAGGAGGGC ACCCTGATTG TCCGCCAGAG TGCAGTTGAC 
CAAAAGCGCG CCAGCCATCA TGAGAGCAAT GGCTTTGCCG GTCGCGTTCA CCTCTTGCCA 
GATCTCTTAC AGCAAAGCCA TTCCTCCTCC ACTTCCTCCA CCTCCTCCTC CCCATCCTCC 
AGCCAGCCGA CACCCACCAT GTCCCCACAG ACACCCCAGG ACAAGCTCAC TCCTAACGAG 
ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA CACAAATCTT CCTCCTCCTT TACACCTTTT 
ATAGACCCCA GATTACTACA GATTTCTCCA TCTAGTGGGA CAACAGTGAC TTCTGTGGTG 
GGATTTTCCT GTGATGGAAT GAGATCGGAG GCCATAAGGC AAGATCCTAC CCGGAAGGGC 
TCAGTGGTCA ATGTGAATCC CACCAACACT AGGCCACAGA GCGACACCCC AGAGATTCGC 
AAATATAAGA AGAGGTTTAA CTCCGAGATT CTGTGCGCTG CCTTGTGGGG AGTGAACTTG 
TTGGTGGGTA CAGAGAGTGG CCTGATGCTG CTGGACAGAA GTGGCCAAGG GAAGGTGTAT 
CCTCTGATCA ACAGAAGACG ATTTCAACAG ATGGACGTCC TGGAAGGCTT GAATGTCTTG 
GTGACAATAT CTGGCAAAAA GGATAAGTTA CGTGTCTACT ATTTGTCCTG GTTAAGAAAT 
AAAATACTTC ACAACGATCC GGAAGTTGAG AAGAAGCAGG GTTGGACGAC CGTGGGAGAT 
TTGGAAGGAT GCGTCCACTA TAAAGTCGTA AAATATGAAA GAATCAAGTT TCTGGTCATT 
GCTTTGAAGA GTTCTGTGGA AGTGTATGCG TGGGCACCCA AGCCATATCA CAAATTCATG 
GCCTTTAAGT CGTTTGGAGA TTTGGTGCAT AAGCCATTAC TGGTGGATCT CACTGTTGAG 
GAAGGCCAGA GGTTGAAAGT GATCTATGGA TCCTGTGCTG GATTCCATGC TGTTGACGTG 
GATTCAGGAT CAGTCTATGA CATTTATCTA CCAACACACG TAAGAAAGAA CCCACACTCT 
ATGATCCAGT GCAGCATCAA ACCCCACGCA ATCATCATCC TCCCCAACAC GGATGGAATG 
GAGCTTCTGG TGTGCTATGA AGACGAAGGG GTCTATGTGA ACACCTATGG AAGGATTACC 
AAGGATGTGG TTCTGCAGTG GGGAGAGATG CCAACGTCTG TAGCGTATAT TCGATCCAAT 
CAGACGATGG GCTGGGGAGA AAAGGCCATA GAGATCCGGT CTGTGGAGAC GGGCCACTTG 
GACGGTGTGT TTATGCACAA AAGGGCTCAA AGACTGAAAT TCTTATGTGA ACGCAACGAC 
AAGGTGTTCT TTGCCTCGGT CCGGTCCGGG GGCAGCAGCC AGGTGTACTT CATGACGCTG 
GGCAGGACCT CTCTTCTGAG CTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_019931221.1 ) cDNA ORF clone, Tursiops truncatus(common bottlenose dolphin) -> XP_019786780.1 Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OTu39180
Clone ID Related Accession (Same CDS sequence)	XM_019931221.1
Accession Version	XM_019931221.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Tursiops truncatus(common bottlenose dolphin)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017842906.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tursiops truncatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
CCGGCTGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
AAGGGTCGAC ACGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA CGTTACTGAG 
GATGAAGAGG AGGAAATCAA GCTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGCGGGGCC GGGTCCATCA CAGACCTCGT GAAGAACACC 
AAGGGCAACA CGCTCAAGGA GGACTGGATA GCCTACATCT CCAGGGAGAT CCTGAGGGGA 
CTGGCGCATC TCCACATCCA TCACGTGATT CACCGGGACA TCAAGGGCCA GAATGTATTG 
CTGACCGAGA ACGCAGAGGT GAAGCTTGTG GATTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACCGTGGGGA GGAGAAACAC CTTCATAGGC ACCCCGTACT GGATGGCTCC CGAGGTCATC 
GCCTGTGACG AGAACCCCGA CGCCACCTAT GATTACAGGA GTGACCTGTG GTCCTGCGGC 
ATCACAGCCA TTGAGATGGC AGAAGGCGCT CCCCCTCTCT GTGACATGCA TCCCATGAGA 
GCACTGTTTC TCATCCCCAG AAACCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCAAAA 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACA 
GAGCAGCTTC TGAAACATCC TTTTATCAGG GACCAGCCGA ATGAAAGGCA GGTTAGGATC 
CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACCGAG 
TACGAGTACA GCGGGAGTGA GGAGGAGGAG GAGGAGGCGC CGGAGCAGGA GGGGGAGCCC 
AGCTCCATCG TCAACGTGCC TGGCGAGTCC ACGCTGCGCC GGGACTTCCT GAGGCTGCAG 
CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGCGGCAGC AGCTCCTGCA GGAGCAGCAG 
CTCAGGGAGC AGGAAGAATA CAAGAGGCAG CTGCTGGCGG AGAGACAGAA GCGGATCGAG 
CAGCAGAAGG AGCAGCGGCG ACGGCTGGAA GAGCAACAAA GGAGAGAGCG GGAGGCCAGG 
AGGCAGCAGG AGCGCGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
CTGGAGAGAC GGCGGCAGGA GGAAGAGGAG CGCAGGCGGG CGGAGGAGGA CAAGAGGAGA 
GTCGACAGGG AGCAGGAGTA TATCAGGCGG CAGCTGGAAG AGGAGCAGCG GCACCTGGAA 
CTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
CTGGAGGAGC AGCGGCAGAC GGAGCGGCTC CAGAGGCAGC TGCAGCAGGA GCAAGCCTAT 
CTCCTGTCTC TGCAGCATGA GCCCCGGAGG CCGCCCCCGC CGCCGGACAG GAGCAAGGCC 
GGCCTGCACG GCGCCGAGCC CAAGGCCCAG CACGAGCCCG CCGACCGGGC CCGCGAGGTG 
GAAGATAGAT TTAGGAAAAC TAACCACAGC TCCCCTGAAG CCCAGTCTAA GCAGACAGGC 
AGAGTATTGG AGCCACCAGT GCCTTCCAGA TCAGAATCTT TTTCCAATGG CAACTCCGAG 
TCTGTGCACC CTGCCCTGCA GAGACCAGCC GAGCCACAGG TACAGTGGTC CCACCTGGCA 
TCTCTCAAGA ACAGTGTTTC CCCTGTCTCG CGATCCCATT CCTTCAGTGA CCCTTCTCCT 
CCCAAATTTG CACACCACCA TCTTCGCTCT CAGGACCCAT GTCCACCTTC CCGCAGTGAG 
GTGCTAAGTC AGAGTTCTGA CTCTAAGTCG GAGGTACCCG ACCCCGCCCA AAAGGCTTGG 
TCTAGATCAG ACAGTGACGA GGTCCCTCCA AGGGTTCCTG TGAGAACGAC GTCCCGGTCC 
CCCGTCCTGT CCCGTCGGGA TTCCCCACTG CAGGGCAGTG GGCAGCAAAA CAGCCAAGCA 
GGTCAGAGAA ACTCCACCAG CAGCATCGAG CCCAGGCTGC TATGGGAGAG AGTGGAGAAG 
CTGGTGCCCA GGCCTGGCAG TGGCAGCTCC TCGGGGTCCA GCAACTCAGG ATCCCAGCCC 
GGCTCCCACC CTGGGTCTCA GAGCGGCTCC GGGGAGCGCT TCAGAGTGAG ATCGTCATCC 
AAATCTGAGG GCTCGCCATC TCAGCGCCTT GAAAATGCAG CAAAAAAGCC TGAAGATAAG 
AAGGAAGTTT TTAGACCTCT CAAGCCCGCT GGCGAAGTGG ACCTGACGGC CCTGGCCAGA 
GAGCTCCGAG CGGCGGAAGA CGTGCGGCCG CCGCACAAGG TGACAGACTA CTCGTCGTCC 
AGCGAGGAGT CCGGGACAAC GGATGAGGAG GACGACGACG TGGAGCAGGA AGGCCCCGAG 
GAGCCCACCT CGGGGCCGGA GGACGCCCGA GCCGCGTCAT CTCTGAACTT GAGCAACGGT 
GAGACGGAGT CCGTGAAAAC GATGATCGTC CACGACGACG TGGAGAGTGA ACCAGCCATG 
ACCCCGTCCA AGGAGGGCAC CCTGATTGTC CGCCAGACTC AGTCCGCTAG TAGCACACTC 
CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGATT ACTACAGATT 
TCTCCATCTA GTGGGACAAC AGTGACTTCT GTGGTGGGAT TTTCCTGTGA TGGAATGAGA 
TCGGAGGCCA TAAGGCAAGA TCCTACCCGG AAGGGCTCAG TGGTCAATGT GAATCCCACC 
AACACTAGGC CACAGAGCGA CACCCCAGAG ATTCGCAAAT ATAAGAAGAG GTTTAACTCC 
GAGATTCTGT GCGCTGCCTT GTGGGGAGTG AACTTGTTGG TGGGTACAGA GAGTGGCCTG 
ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG GTGTATCCTC TGATCAACAG AAGACGATTT 
CAACAGATGG ACGTCCTGGA AGGCTTGAAT GTCTTGGTGA CAATATCTGG CAAAAAGGAT 
AAGTTACGTG TCTACTATTT GTCCTGGTTA AGAAATAAAA TACTTCACAA CGATCCGGAA 
GTTGAGAAGA AGCAGGGTTG GACGACCGTG GGAGATTTGG AAGGATGCGT CCACTATAAA 
GTCGTAAAAT ATGAAAGAAT CAAGTTTCTG GTCATTGCTT TGAAGAGTTC TGTGGAAGTG 
TATGCGTGGG CACCCAAGCC ATATCACAAA TTCATGGCCT TTAAGTCGTT TGGAGATTTG 
GTGCATAAGC CATTACTGGT GGATCTCACT GTTGAGGAAG GCCAGAGGTT GAAAGTGATC 
TATGGATCCT GTGCTGGATT CCATGCTGTT GACGTGGATT CAGGATCAGT CTATGACATT 
TATCTACCAA CACACATCCA GTGCAGCATC AAACCCCACG CAATCATCAT CCTCCCCAAC 
ACGGATGGAA TGGAGCTTCT GGTGTGCTAT GAAGACGAAG GGGTCTATGT GAACACCTAT 
GGAAGGATTA CCAAGGATGT GGTTCTGCAG TGGGGAGAGA TGCCAACGTC TGTAGCGTAT 
ATTCGATCCA ATCAGACGAT GGGCTGGGGA GAAAAGGCCA TAGAGATCCG GTCTGTGGAG 
ACGGGCCACT TGGACGGTGT GTTTATGCAC AAAAGGGCTC AAAGACTGAA ATTCTTATGT 
GAACGCAACG ACAAGGTGTT CTTTGCCTCG GTCCGGTCCG GGGGCAGCAG CCAGGTGTAC 
TTCATGACGC TGGGCAGGAC CTCTCTTCTG AGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019786780.1
CDS	148..4086
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEAPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRQEEEERRRAEEDKRRVDREQEYIRRQLEEEQRHLELLQQQLLQEQAMLLECRWRELEEQRQTERLQRQLQQEQAYLLSLQHEPRRPPPPPDRSKAGLHGAEPKAQHEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVQWSHLASLKNSVSPVSRSHSFSDPSPPKFAHHHLRSQDPCPPSRSEVLSQSSDSKSEVPDPAQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPAGEVDLTALARELRAAEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGPEEPTSGPEDARAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRSEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGDLVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_019931221.1
Organism	Tursiops truncatus(common bottlenose dolphin)
Definition	Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019931221.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
CCGGCTGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
AAGGGTCGAC ACGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA CGTTACTGAG 
GATGAAGAGG AGGAAATCAA GCTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGCGGGGCC GGGTCCATCA CAGACCTCGT GAAGAACACC 
AAGGGCAACA CGCTCAAGGA GGACTGGATA GCCTACATCT CCAGGGAGAT CCTGAGGGGA 
CTGGCGCATC TCCACATCCA TCACGTGATT CACCGGGACA TCAAGGGCCA GAATGTATTG 
CTGACCGAGA ACGCAGAGGT GAAGCTTGTG GATTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACCGTGGGGA GGAGAAACAC CTTCATAGGC ACCCCGTACT GGATGGCTCC CGAGGTCATC 
GCCTGTGACG AGAACCCCGA CGCCACCTAT GATTACAGGA GTGACCTGTG GTCCTGCGGC 
ATCACAGCCA TTGAGATGGC AGAAGGCGCT CCCCCTCTCT GTGACATGCA TCCCATGAGA 
GCACTGTTTC TCATCCCCAG AAACCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCAAAA 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACA 
GAGCAGCTTC TGAAACATCC TTTTATCAGG GACCAGCCGA ATGAAAGGCA GGTTAGGATC 
CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACCGAG 
TACGAGTACA GCGGGAGTGA GGAGGAGGAG GAGGAGGCGC CGGAGCAGGA GGGGGAGCCC 
AGCTCCATCG TCAACGTGCC TGGCGAGTCC ACGCTGCGCC GGGACTTCCT GAGGCTGCAG 
CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGCGGCAGC AGCTCCTGCA GGAGCAGCAG 
CTCAGGGAGC AGGAAGAATA CAAGAGGCAG CTGCTGGCGG AGAGACAGAA GCGGATCGAG 
CAGCAGAAGG AGCAGCGGCG ACGGCTGGAA GAGCAACAAA GGAGAGAGCG GGAGGCCAGG 
AGGCAGCAGG AGCGCGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
CTGGAGAGAC GGCGGCAGGA GGAAGAGGAG CGCAGGCGGG CGGAGGAGGA CAAGAGGAGA 
GTCGACAGGG AGCAGGAGTA TATCAGGCGG CAGCTGGAAG AGGAGCAGCG GCACCTGGAA 
CTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
CTGGAGGAGC AGCGGCAGAC GGAGCGGCTC CAGAGGCAGC TGCAGCAGGA GCAAGCCTAT 
CTCCTGTCTC TGCAGCATGA GCCCCGGAGG CCGCCCCCGC CGCCGGACAG GAGCAAGGCC 
GGCCTGCACG GCGCCGAGCC CAAGGCCCAG CACGAGCCCG CCGACCGGGC CCGCGAGGTG 
GAAGATAGAT TTAGGAAAAC TAACCACAGC TCCCCTGAAG CCCAGTCTAA GCAGACAGGC 
AGAGTATTGG AGCCACCAGT GCCTTCCAGA TCAGAATCTT TTTCCAATGG CAACTCCGAG 
TCTGTGCACC CTGCCCTGCA GAGACCAGCC GAGCCACAGG TACAGTGGTC CCACCTGGCA 
TCTCTCAAGA ACAGTGTTTC CCCTGTCTCG CGATCCCATT CCTTCAGTGA CCCTTCTCCT 
CCCAAATTTG CACACCACCA TCTTCGCTCT CAGGACCCAT GTCCACCTTC CCGCAGTGAG 
GTGCTAAGTC AGAGTTCTGA CTCTAAGTCG GAGGTACCCG ACCCCGCCCA AAAGGCTTGG 
TCTAGATCAG ACAGTGACGA GGTCCCTCCA AGGGTTCCTG TGAGAACGAC GTCCCGGTCC 
CCCGTCCTGT CCCGTCGGGA TTCCCCACTG CAGGGCAGTG GGCAGCAAAA CAGCCAAGCA 
GGTCAGAGAA ACTCCACCAG CAGCATCGAG CCCAGGCTGC TATGGGAGAG AGTGGAGAAG 
CTGGTGCCCA GGCCTGGCAG TGGCAGCTCC TCGGGGTCCA GCAACTCAGG ATCCCAGCCC 
GGCTCCCACC CTGGGTCTCA GAGCGGCTCC GGGGAGCGCT TCAGAGTGAG ATCGTCATCC 
AAATCTGAGG GCTCGCCATC TCAGCGCCTT GAAAATGCAG CAAAAAAGCC TGAAGATAAG 
AAGGAAGTTT TTAGACCTCT CAAGCCCGCT GGCGAAGTGG ACCTGACGGC CCTGGCCAGA 
GAGCTCCGAG CGGCGGAAGA CGTGCGGCCG CCGCACAAGG TGACAGACTA CTCGTCGTCC 
AGCGAGGAGT CCGGGACAAC GGATGAGGAG GACGACGACG TGGAGCAGGA AGGCCCCGAG 
GAGCCCACCT CGGGGCCGGA GGACGCCCGA GCCGCGTCAT CTCTGAACTT GAGCAACGGT 
GAGACGGAGT CCGTGAAAAC GATGATCGTC CACGACGACG TGGAGAGTGA ACCAGCCATG 
ACCCCGTCCA AGGAGGGCAC CCTGATTGTC CGCCAGACTC AGTCCGCTAG TAGCACACTC 
CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGATT ACTACAGATT 
TCTCCATCTA GTGGGACAAC AGTGACTTCT GTGGTGGGAT TTTCCTGTGA TGGAATGAGA 
TCGGAGGCCA TAAGGCAAGA TCCTACCCGG AAGGGCTCAG TGGTCAATGT GAATCCCACC 
AACACTAGGC CACAGAGCGA CACCCCAGAG ATTCGCAAAT ATAAGAAGAG GTTTAACTCC 
GAGATTCTGT GCGCTGCCTT GTGGGGAGTG AACTTGTTGG TGGGTACAGA GAGTGGCCTG 
ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG GTGTATCCTC TGATCAACAG AAGACGATTT 
CAACAGATGG ACGTCCTGGA AGGCTTGAAT GTCTTGGTGA CAATATCTGG CAAAAAGGAT 
AAGTTACGTG TCTACTATTT GTCCTGGTTA AGAAATAAAA TACTTCACAA CGATCCGGAA 
GTTGAGAAGA AGCAGGGTTG GACGACCGTG GGAGATTTGG AAGGATGCGT CCACTATAAA 
GTCGTAAAAT ATGAAAGAAT CAAGTTTCTG GTCATTGCTT TGAAGAGTTC TGTGGAAGTG 
TATGCGTGGG CACCCAAGCC ATATCACAAA TTCATGGCCT TTAAGTCGTT TGGAGATTTG 
GTGCATAAGC CATTACTGGT GGATCTCACT GTTGAGGAAG GCCAGAGGTT GAAAGTGATC 
TATGGATCCT GTGCTGGATT CCATGCTGTT GACGTGGATT CAGGATCAGT CTATGACATT 
TATCTACCAA CACACATCCA GTGCAGCATC AAACCCCACG CAATCATCAT CCTCCCCAAC 
ACGGATGGAA TGGAGCTTCT GGTGTGCTAT GAAGACGAAG GGGTCTATGT GAACACCTAT 
GGAAGGATTA CCAAGGATGT GGTTCTGCAG TGGGGAGAGA TGCCAACGTC TGTAGCGTAT 
ATTCGATCCA ATCAGACGAT GGGCTGGGGA GAAAAGGCCA TAGAGATCCG GTCTGTGGAG 
ACGGGCCACT TGGACGGTGT GTTTATGCAC AAAAGGGCTC AAAGACTGAA ATTCTTATGT 
GAACGCAACG ACAAGGTGTT CTTTGCCTCG GTCCGGTCCG GGGGCAGCAG CCAGGTGTAC 
TTCATGACGC TGGGCAGGAC CTCTCTTCTG AGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_019931222.1 ) cDNA ORF clone, Tursiops truncatus(common bottlenose dolphin) -> XP_019786781.1 Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X5, mRNA.

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CloneID	OTu39181
Clone ID Related Accession (Same CDS sequence)	XM_019931222.1
Accession Version	XM_019931222.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Tursiops truncatus(common bottlenose dolphin)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017842906.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tursiops truncatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
CCGGCTGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
AAGGGTCGAC ACGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA CGTTACTGAG 
GATGAAGAGG AGGAAATCAA GCTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGCGGGGCC GGGTCCATCA CAGACCTCGT GAAGAACACC 
AAGGGCAACA CGCTCAAGGA GGACTGGATA GCCTACATCT CCAGGGAGAT CCTGAGGGGA 
CTGGCGCATC TCCACATCCA TCACGTGATT CACCGGGACA TCAAGGGCCA GAATGTATTG 
CTGACCGAGA ACGCAGAGGT GAAGCTTGTG GATTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACCGTGGGGA GGAGAAACAC CTTCATAGGC ACCCCGTACT GGATGGCTCC CGAGGTCATC 
GCCTGTGACG AGAACCCCGA CGCCACCTAT GATTACAGGA GTGACCTGTG GTCCTGCGGC 
ATCACAGCCA TTGAGATGGC AGAAGGCGCT CCCCCTCTCT GTGACATGCA TCCCATGAGA 
GCACTGTTTC TCATCCCCAG AAACCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCAAAA 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACA 
GAGCAGCTTC TGAAACATCC TTTTATCAGG GACCAGCCGA ATGAAAGGCA GGTTAGGATC 
CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACCGAG 
TACGAGTACA GCGGGAGTGA GGAGGAGGAG GAGGAGGCGC CGGAGCAGGA GGGGGAGCCC 
AGCTCCATCG TCAACGTGCC TGGCGAGTCC ACGCTGCGCC GGGACTTCCT GAGGCTGCAG 
CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGCGGCAGC AGCTCCTGCA GGAGCAGCAG 
CTCAGGGAGC AGGAAGAATA CAAGAGGCAG CTGCTGGCGG AGAGACAGAA GCGGATCGAG 
CAGCAGAAGG AGCAGCGGCG ACGGCTGGAA GAGCAACAAA GGAGAGAGCG GGAGGCCAGG 
AGGCAGCAGG AGCGCGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
CTGGAGAGAC GGCGGCAGGA GGAAGAGGAG CGCAGGCGGG CGGAGGAGGA CAAGAGGAGA 
GTCGACAGGG AGCAGGAGTA TATCAGGCGG CAGCTGGAAG AGGAGCAGCG GCACCTGGAA 
CTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
CTGGAGGAGC AGCGGCAGAC GGAGCGGCTC CAGAGGCAGC TGCAGCAGGA GCAAGCCTAT 
CTCCTGTCTC TGCAGCATGA GCCCCGGAGG CCGCCCCCGC CGCCGGACAG GAGCAAGGCC 
GGCCTGCACG GCGCCGAGCC CAAGGCCCAG CACGAGCCCG CCGACCGGGC CCGCGAGGTG 
GAAGATAGAT TTAGGAAAAC TAACCACAGC TCCCCTGAAG CCCAGTCTAA GCAGACAGGC 
AGAGTATTGG AGCCACCAGT GCCTTCCAGA TCAGAATCTT TTTCCAATGG CAACTCCGAG 
TCTGTGCACC CTGCCCTGCA GAGACCAGCC GAGCCACAGG TTCCTGTGAG AACGACGTCC 
CGGTCCCCCG TCCTGTCCCG TCGGGATTCC CCACTGCAGG GCAGTGGGCA GCAAAACAGC 
CAAGCAGGTC AGAGAAACTC CACCAGCAGC ATCGAGCCCA GGCTGCTATG GGAGAGAGTG 
GAGAAGCTGG TGCCCAGGCC TGGCAGTGGC AGCTCCTCGG GGTCCAGCAA CTCAGGATCC 
CAGCCCGGCT CCCACCCTGG GTCTCAGAGC GGCTCCGGGG AGCGCTTCAG AGTGAGATCG 
TCATCCAAAT CTGAGGGCTC GCCATCTCAG CGCCTTGAAA ATGCAGCAAA AAAGCCTGAA 
GATAAGAAGG AAGTTTTTAG ACCTCTCAAG CCCGCTGGCG AAGTGGACCT GACGGCCCTG 
GCCAGAGAGC TCCGAGCGGC GGAAGACGTG CGGCCGCCGC ACAAGGTGAC AGACTACTCG 
TCGTCCAGCG AGGAGTCCGG GACAACGGAT GAGGAGGACG ACGACGTGGA GCAGGAAGGC 
CCCGAGGAGC CCACCTCGGG GCCGGAGGAC GCCCGAGCCG CGTCATCTCT GAACTTGAGC 
AACGGTGAGA CGGAGTCCGT GAAAACGATG ATCGTCCACG ACGACGTGGA GAGTGAACCA 
GCCATGACCC CGTCCAAGGA GGGCACCCTG ATTGTCCGCC AGAGTGCAGT TGACCAAAAG 
CGCGCCAGCC ATCATGAGAG CAATGGCTTT GCCGGTCGCG TTCACCTCTT GCCAGATCTC 
TTACAGCAAA GCCATTCCTC CTCCACTTCC TCCACCTCCT CCTCCCCATC CTCCAGCCAG 
CCGACACCCA CCATGTCCCC ACAGACACCC CAGGACAAGC TCACTCCTAA CGAGACTCAG 
TCCGCTAGTA GCACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC 
CCCAGATTAC TACAGATTTC TCCATCTAGT GGGACAACAG TGACTTCTGT GGTGGGATTT 
TCCTGTGATG GAATGAGATC GGAGGCCATA AGGCAAGATC CTACCCGGAA GGGCTCAGTG 
GTCAATGTGA ATCCCACCAA CACTAGGCCA CAGAGCGACA CCCCAGAGAT TCGCAAATAT 
AAGAAGAGGT TTAACTCCGA GATTCTGTGC GCTGCCTTGT GGGGAGTGAA CTTGTTGGTG 
GGTACAGAGA GTGGCCTGAT GCTGCTGGAC AGAAGTGGCC AAGGGAAGGT GTATCCTCTG 
ATCAACAGAA GACGATTTCA ACAGATGGAC GTCCTGGAAG GCTTGAATGT CTTGGTGACA 
ATATCTGGCA AAAAGGATAA GTTACGTGTC TACTATTTGT CCTGGTTAAG AAATAAAATA 
CTTCACAACG ATCCGGAAGT TGAGAAGAAG CAGGGTTGGA CGACCGTGGG AGATTTGGAA 
GGATGCGTCC ACTATAAAGT CGTAAAATAT GAAAGAATCA AGTTTCTGGT CATTGCTTTG 
AAGAGTTCTG TGGAAGTGTA TGCGTGGGCA CCCAAGCCAT ATCACAAATT CATGGCCTTT 
AAGTCGTTTG GAGATTTGGT GCATAAGCCA TTACTGGTGG ATCTCACTGT TGAGGAAGGC 
CAGAGGTTGA AAGTGATCTA TGGATCCTGT GCTGGATTCC ATGCTGTTGA CGTGGATTCA 
GGATCAGTCT ATGACATTTA TCTACCAACA CACGTAAGAA AGAACCCACA CTCTATGATC 
CAGTGCAGCA TCAAACCCCA CGCAATCATC ATCCTCCCCA ACACGGATGG AATGGAGCTT 
CTGGTGTGCT ATGAAGACGA AGGGGTCTAT GTGAACACCT ATGGAAGGAT TACCAAGGAT 
GTGGTTCTGC AGTGGGGAGA GATGCCAACG TCTGTAGCGT ATATTCGATC CAATCAGACG 
ATGGGCTGGG GAGAAAAGGC CATAGAGATC CGGTCTGTGG AGACGGGCCA CTTGGACGGT 
GTGTTTATGC ACAAAAGGGC TCAAAGACTG AAATTCTTAT GTGAACGCAA CGACAAGGTG 
TTCTTTGCCT CGGTCCGGTC CGGGGGCAGC AGCCAGGTGT ACTTCATGAC GCTGGGCAGG 
ACCTCTCTTC TGAGCTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019786781.1
CDS	149..4069
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEAPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRQEEEERRRAEEDKRRVDREQEYIRRQLEEEQRHLELLQQQLLQEQAMLLECRWRELEEQRQTERLQRQLQQEQAYLLSLQHEPRRPPPPPDRSKAGLHGAEPKAQHEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPAGEVDLTALARELRAAEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGPEEPTSGPEDARAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSAVDQKRASHHESNGFAGRVHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTPNETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRSEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGDLVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_019931222.1
Organism	Tursiops truncatus(common bottlenose dolphin)
Definition	Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019931222.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
CCGGCTGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
AAGGGTCGAC ACGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA CGTTACTGAG 
GATGAAGAGG AGGAAATCAA GCTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGCGGGGCC GGGTCCATCA CAGACCTCGT GAAGAACACC 
AAGGGCAACA CGCTCAAGGA GGACTGGATA GCCTACATCT CCAGGGAGAT CCTGAGGGGA 
CTGGCGCATC TCCACATCCA TCACGTGATT CACCGGGACA TCAAGGGCCA GAATGTATTG 
CTGACCGAGA ACGCAGAGGT GAAGCTTGTG GATTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACCGTGGGGA GGAGAAACAC CTTCATAGGC ACCCCGTACT GGATGGCTCC CGAGGTCATC 
GCCTGTGACG AGAACCCCGA CGCCACCTAT GATTACAGGA GTGACCTGTG GTCCTGCGGC 
ATCACAGCCA TTGAGATGGC AGAAGGCGCT CCCCCTCTCT GTGACATGCA TCCCATGAGA 
GCACTGTTTC TCATCCCCAG AAACCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCAAAA 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACA 
GAGCAGCTTC TGAAACATCC TTTTATCAGG GACCAGCCGA ATGAAAGGCA GGTTAGGATC 
CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACCGAG 
TACGAGTACA GCGGGAGTGA GGAGGAGGAG GAGGAGGCGC CGGAGCAGGA GGGGGAGCCC 
AGCTCCATCG TCAACGTGCC TGGCGAGTCC ACGCTGCGCC GGGACTTCCT GAGGCTGCAG 
CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGCGGCAGC AGCTCCTGCA GGAGCAGCAG 
CTCAGGGAGC AGGAAGAATA CAAGAGGCAG CTGCTGGCGG AGAGACAGAA GCGGATCGAG 
CAGCAGAAGG AGCAGCGGCG ACGGCTGGAA GAGCAACAAA GGAGAGAGCG GGAGGCCAGG 
AGGCAGCAGG AGCGCGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
CTGGAGAGAC GGCGGCAGGA GGAAGAGGAG CGCAGGCGGG CGGAGGAGGA CAAGAGGAGA 
GTCGACAGGG AGCAGGAGTA TATCAGGCGG CAGCTGGAAG AGGAGCAGCG GCACCTGGAA 
CTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
CTGGAGGAGC AGCGGCAGAC GGAGCGGCTC CAGAGGCAGC TGCAGCAGGA GCAAGCCTAT 
CTCCTGTCTC TGCAGCATGA GCCCCGGAGG CCGCCCCCGC CGCCGGACAG GAGCAAGGCC 
GGCCTGCACG GCGCCGAGCC CAAGGCCCAG CACGAGCCCG CCGACCGGGC CCGCGAGGTG 
GAAGATAGAT TTAGGAAAAC TAACCACAGC TCCCCTGAAG CCCAGTCTAA GCAGACAGGC 
AGAGTATTGG AGCCACCAGT GCCTTCCAGA TCAGAATCTT TTTCCAATGG CAACTCCGAG 
TCTGTGCACC CTGCCCTGCA GAGACCAGCC GAGCCACAGG TTCCTGTGAG AACGACGTCC 
CGGTCCCCCG TCCTGTCCCG TCGGGATTCC CCACTGCAGG GCAGTGGGCA GCAAAACAGC 
CAAGCAGGTC AGAGAAACTC CACCAGCAGC ATCGAGCCCA GGCTGCTATG GGAGAGAGTG 
GAGAAGCTGG TGCCCAGGCC TGGCAGTGGC AGCTCCTCGG GGTCCAGCAA CTCAGGATCC 
CAGCCCGGCT CCCACCCTGG GTCTCAGAGC GGCTCCGGGG AGCGCTTCAG AGTGAGATCG 
TCATCCAAAT CTGAGGGCTC GCCATCTCAG CGCCTTGAAA ATGCAGCAAA AAAGCCTGAA 
GATAAGAAGG AAGTTTTTAG ACCTCTCAAG CCCGCTGGCG AAGTGGACCT GACGGCCCTG 
GCCAGAGAGC TCCGAGCGGC GGAAGACGTG CGGCCGCCGC ACAAGGTGAC AGACTACTCG 
TCGTCCAGCG AGGAGTCCGG GACAACGGAT GAGGAGGACG ACGACGTGGA GCAGGAAGGC 
CCCGAGGAGC CCACCTCGGG GCCGGAGGAC GCCCGAGCCG CGTCATCTCT GAACTTGAGC 
AACGGTGAGA CGGAGTCCGT GAAAACGATG ATCGTCCACG ACGACGTGGA GAGTGAACCA 
GCCATGACCC CGTCCAAGGA GGGCACCCTG ATTGTCCGCC AGAGTGCAGT TGACCAAAAG 
CGCGCCAGCC ATCATGAGAG CAATGGCTTT GCCGGTCGCG TTCACCTCTT GCCAGATCTC 
TTACAGCAAA GCCATTCCTC CTCCACTTCC TCCACCTCCT CCTCCCCATC CTCCAGCCAG 
CCGACACCCA CCATGTCCCC ACAGACACCC CAGGACAAGC TCACTCCTAA CGAGACTCAG 
TCCGCTAGTA GCACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC 
CCCAGATTAC TACAGATTTC TCCATCTAGT GGGACAACAG TGACTTCTGT GGTGGGATTT 
TCCTGTGATG GAATGAGATC GGAGGCCATA AGGCAAGATC CTACCCGGAA GGGCTCAGTG 
GTCAATGTGA ATCCCACCAA CACTAGGCCA CAGAGCGACA CCCCAGAGAT TCGCAAATAT 
AAGAAGAGGT TTAACTCCGA GATTCTGTGC GCTGCCTTGT GGGGAGTGAA CTTGTTGGTG 
GGTACAGAGA GTGGCCTGAT GCTGCTGGAC AGAAGTGGCC AAGGGAAGGT GTATCCTCTG 
ATCAACAGAA GACGATTTCA ACAGATGGAC GTCCTGGAAG GCTTGAATGT CTTGGTGACA 
ATATCTGGCA AAAAGGATAA GTTACGTGTC TACTATTTGT CCTGGTTAAG AAATAAAATA 
CTTCACAACG ATCCGGAAGT TGAGAAGAAG CAGGGTTGGA CGACCGTGGG AGATTTGGAA 
GGATGCGTCC ACTATAAAGT CGTAAAATAT GAAAGAATCA AGTTTCTGGT CATTGCTTTG 
AAGAGTTCTG TGGAAGTGTA TGCGTGGGCA CCCAAGCCAT ATCACAAATT CATGGCCTTT 
AAGTCGTTTG GAGATTTGGT GCATAAGCCA TTACTGGTGG ATCTCACTGT TGAGGAAGGC 
CAGAGGTTGA AAGTGATCTA TGGATCCTGT GCTGGATTCC ATGCTGTTGA CGTGGATTCA 
GGATCAGTCT ATGACATTTA TCTACCAACA CACGTAAGAA AGAACCCACA CTCTATGATC 
CAGTGCAGCA TCAAACCCCA CGCAATCATC ATCCTCCCCA ACACGGATGG AATGGAGCTT 
CTGGTGTGCT ATGAAGACGA AGGGGTCTAT GTGAACACCT ATGGAAGGAT TACCAAGGAT 
GTGGTTCTGC AGTGGGGAGA GATGCCAACG TCTGTAGCGT ATATTCGATC CAATCAGACG 
ATGGGCTGGG GAGAAAAGGC CATAGAGATC CGGTCTGTGG AGACGGGCCA CTTGGACGGT 
GTGTTTATGC ACAAAAGGGC TCAAAGACTG AAATTCTTAT GTGAACGCAA CGACAAGGTG 
TTCTTTGCCT CGGTCCGGTC CGGGGGCAGC AGCCAGGTGT ACTTCATGAC GCTGGGCAGG 
ACCTCTCTTC TGAGCTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_019931223.1 ) cDNA ORF clone, Tursiops truncatus(common bottlenose dolphin) -> XP_019786782.1 Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X6, mRNA.

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CloneID	OTu39182
Clone ID Related Accession (Same CDS sequence)	XM_019931223.1
Accession Version	XM_019931223.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Tursiops truncatus(common bottlenose dolphin)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017842906.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tursiops truncatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
CCGGCTGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
AAGGGTCGAC ACGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA CGTTACTGAG 
GATGAAGAGG AGGAAATCAA GCTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGCGGGGCC GGGTCCATCA CAGACCTCGT GAAGAACACC 
AAGGGCAACA CGCTCAAGGA GGACTGGATA GCCTACATCT CCAGGGAGAT CCTGAGGGGA 
CTGGCGCATC TCCACATCCA TCACGTGATT CACCGGGACA TCAAGGGCCA GAATGTATTG 
CTGACCGAGA ACGCAGAGGT GAAGCTTGTG GATTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACCGTGGGGA GGAGAAACAC CTTCATAGGC ACCCCGTACT GGATGGCTCC CGAGGTCATC 
GCCTGTGACG AGAACCCCGA CGCCACCTAT GATTACAGGA GTGACCTGTG GTCCTGCGGC 
ATCACAGCCA TTGAGATGGC AGAAGGCGCT CCCCCTCTCT GTGACATGCA TCCCATGAGA 
GCACTGTTTC TCATCCCCAG AAACCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCAAAA 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACA 
GAGCAGCTTC TGAAACATCC TTTTATCAGG GACCAGCCGA ATGAAAGGCA GGTTAGGATC 
CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACCGAG 
TACGAGTACA GCGGGAGTGA GGAGGAGGAG GAGGAGGCGC CGGAGCAGGA GGGGGAGCCC 
AGCTCCATCG TCAACGTGCC TGGCGAGTCC ACGCTGCGCC GGGACTTCCT GAGGCTGCAG 
CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGCGGCAGC AGCTCCTGCA GGAGCAGCAG 
CTCAGGGAGC AGGAAGAATA CAAGAGGCAG CTGCTGGCGG AGAGACAGAA GCGGATCGAG 
CAGCAGAAGG AGCAGCGGCG ACGGCTGGAA GAGCAACAAA GGAGAGAGCG GGAGGCCAGG 
AGGCAGCAGG AGCGCGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
CTGGAGAGAC GGCGGCAGGA GGAAGAGGAG CGCAGGCGGG CGGAGGAGGA CAAGAGGAGA 
GTCGACAGGG AGCAGGAGTA TATCAGGCGG CAGCTGGAAG AGGAGCAGCG GCACCTGGAA 
CTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCATGAGCC CCGGAGGCCG 
CCCCCGCCGC CGGACAGGAG CAAGGCCGGC CTGCACGGCG CCGAGCCCAA GGCCCAGCAC 
GAGCCCGCCG ACCGGGCCCG CGAGGTACAG TGGTCCCACC TGGCATCTCT CAAGAACAGT 
GTTTCCCCTG TCTCGCGATC CCATTCCTTC AGTGACCCTT CTCCTCCCAA ATTTGCACAC 
CACCATCTTC GCTCTCAGGA CCCATGTCCA CCTTCCCGCA GTGAGGTGCT AAGTCAGAGT 
TCTGACTCTA AGTCGGAGGT ACCCGACCCC GCCCAAAAGG CTTGGTCTAG ATCAGACAGT 
GACGAGGTCC CTCCAAGGGT TCCTGTGAGA ACGACGTCCC GGTCCCCCGT CCTGTCCCGT 
CGGGATTCCC CACTGCAGGG CAGTGGGCAG CAAAACAGCC AAGCAGGTCA GAGAAACTCC 
ACCAGCAGCA TCGAGCCCAG GCTGCTATGG GAGAGAGTGG AGAAGCTGGT GCCCAGGCCT 
GGCAGTGGCA GCTCCTCGGG GTCCAGCAAC TCAGGATCCC AGCCCGGCTC CCACCCTGGG 
TCTCAGAGCG GCTCCGGGGA GCGCTTCAGA GTGAGATCGT CATCCAAATC TGAGGGCTCG 
CCATCTCAGC GCCTTGAAAA TGCAGCAAAA AAGCCTGAAG ATAAGAAGGA AGTTTTTAGA 
CCTCTCAAGC CCGCTGGCGA AGTGGACCTG ACGGCCCTGG CCAGAGAGCT CCGAGCGGCG 
GAAGACGTGC GGCCGCCGCA CAAGGTGACA GACTACTCGT CGTCCAGCGA GGAGTCCGGG 
ACAACGGATG AGGAGGACGA CGACGTGGAG CAGGAAGGCC CCGAGGAGCC CACCTCGGGG 
CCGGAGGACG CCCGAGCCGC GTCATCTCTG AACTTGAGCA ACGGTGAGAC GGAGTCCGTG 
AAAACGATGA TCGTCCACGA CGACGTGGAG AGTGAACCAG CCATGACCCC GTCCAAGGAG 
GGCACCCTGA TTGTCCGCCA GAGTGCAGTT GACCAAAAGC GCGCCAGCCA TCATGAGAGC 
AATGGCTTTG CCGGTCGCGT TCACCTCTTG CCAGATCTCT TACAGCAAAG CCATTCCTCC 
TCCACTTCCT CCACCTCCTC CTCCCCATCC TCCAGCCAGC CGACACCCAC CATGTCCCCA 
CAGACACCCC AGGACAAGCT CACTCCTAAC GAGACTCAGT CCGCTAGTAG CACACTCCAG 
AAACACAAAT CTTCCTCCTC CTTTACACCT TTTATAGACC CCAGATTACT ACAGATTTCT 
CCATCTAGTG GGACAACAGT GACTTCTGTG GTGGGATTTT CCTGTGATGG AATGAGATCG 
GAGGCCATAA GGCAAGATCC TACCCGGAAG GGCTCAGTGG TCAATGTGAA TCCCACCAAC 
ACTAGGCCAC AGAGCGACAC CCCAGAGATT CGCAAATATA AGAAGAGGTT TAACTCCGAG 
ATTCTGTGCG CTGCCTTGTG GGGAGTGAAC TTGTTGGTGG GTACAGAGAG TGGCCTGATG 
CTGCTGGACA GAAGTGGCCA AGGGAAGGTG TATCCTCTGA TCAACAGAAG ACGATTTCAA 
CAGATGGACG TCCTGGAAGG CTTGAATGTC TTGGTGACAA TATCTGGCAA AAAGGATAAG 
TTACGTGTCT ACTATTTGTC CTGGTTAAGA AATAAAATAC TTCACAACGA TCCGGAAGTT 
GAGAAGAAGC AGGGTTGGAC GACCGTGGGA GATTTGGAAG GATGCGTCCA CTATAAAGTC 
GTAAAATATG AAAGAATCAA GTTTCTGGTC ATTGCTTTGA AGAGTTCTGT GGAAGTGTAT 
GCGTGGGCAC CCAAGCCATA TCACAAATTC ATGGCCTTTA AGTCGTTTGG AGATTTGGTG 
CATAAGCCAT TACTGGTGGA TCTCACTGTT GAGGAAGGCC AGAGGTTGAA AGTGATCTAT 
GGATCCTGTG CTGGATTCCA TGCTGTTGAC GTGGATTCAG GATCAGTCTA TGACATTTAT 
CTACCAACAC ACATCCAGTG CAGCATCAAA CCCCACGCAA TCATCATCCT CCCCAACACG 
GATGGAATGG AGCTTCTGGT GTGCTATGAA GACGAAGGGG TCTATGTGAA CACCTATGGA 
AGGATTACCA AGGATGTGGT TCTGCAGTGG GGAGAGATGC CAACGTCTGT AGCGTATATT 
CGATCCAATC AGACGATGGG CTGGGGAGAA AAGGCCATAG AGATCCGGTC TGTGGAGACG 
GGCCACTTGG ACGGTGTGTT TATGCACAAA AGGGCTCAAA GACTGAAATT CTTATGTGAA 
CGCAACGACA AGGTGTTCTT TGCCTCGGTC CGGTCCGGGG GCAGCAGCCA GGTGTACTTC 
ATGACGCTGG GCAGGACCTC TCTTCTGAGC TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019786782.1
CDS	148..4023
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEAPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRQEEEERRRAEEDKRRVDREQEYIRRQLEEEQRHLELLQQQLLQEQAMLLHEPRRPPPPPDRSKAGLHGAEPKAQHEPADRAREVQWSHLASLKNSVSPVSRSHSFSDPSPPKFAHHHLRSQDPCPPSRSEVLSQSSDSKSEVPDPAQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPAGEVDLTALARELRAAEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGPEEPTSGPEDARAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSAVDQKRASHHESNGFAGRVHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTPNETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRSEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGDLVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_019931223.1
Organism	Tursiops truncatus(common bottlenose dolphin)
Definition	Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019931223.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
CCGGCTGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
AAGGGTCGAC ACGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA CGTTACTGAG 
GATGAAGAGG AGGAAATCAA GCTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGCGGGGCC GGGTCCATCA CAGACCTCGT GAAGAACACC 
AAGGGCAACA CGCTCAAGGA GGACTGGATA GCCTACATCT CCAGGGAGAT CCTGAGGGGA 
CTGGCGCATC TCCACATCCA TCACGTGATT CACCGGGACA TCAAGGGCCA GAATGTATTG 
CTGACCGAGA ACGCAGAGGT GAAGCTTGTG GATTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACCGTGGGGA GGAGAAACAC CTTCATAGGC ACCCCGTACT GGATGGCTCC CGAGGTCATC 
GCCTGTGACG AGAACCCCGA CGCCACCTAT GATTACAGGA GTGACCTGTG GTCCTGCGGC 
ATCACAGCCA TTGAGATGGC AGAAGGCGCT CCCCCTCTCT GTGACATGCA TCCCATGAGA 
GCACTGTTTC TCATCCCCAG AAACCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCAAAA 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACA 
GAGCAGCTTC TGAAACATCC TTTTATCAGG GACCAGCCGA ATGAAAGGCA GGTTAGGATC 
CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACCGAG 
TACGAGTACA GCGGGAGTGA GGAGGAGGAG GAGGAGGCGC CGGAGCAGGA GGGGGAGCCC 
AGCTCCATCG TCAACGTGCC TGGCGAGTCC ACGCTGCGCC GGGACTTCCT GAGGCTGCAG 
CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGCGGCAGC AGCTCCTGCA GGAGCAGCAG 
CTCAGGGAGC AGGAAGAATA CAAGAGGCAG CTGCTGGCGG AGAGACAGAA GCGGATCGAG 
CAGCAGAAGG AGCAGCGGCG ACGGCTGGAA GAGCAACAAA GGAGAGAGCG GGAGGCCAGG 
AGGCAGCAGG AGCGCGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
CTGGAGAGAC GGCGGCAGGA GGAAGAGGAG CGCAGGCGGG CGGAGGAGGA CAAGAGGAGA 
GTCGACAGGG AGCAGGAGTA TATCAGGCGG CAGCTGGAAG AGGAGCAGCG GCACCTGGAA 
CTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCATGAGCC CCGGAGGCCG 
CCCCCGCCGC CGGACAGGAG CAAGGCCGGC CTGCACGGCG CCGAGCCCAA GGCCCAGCAC 
GAGCCCGCCG ACCGGGCCCG CGAGGTACAG TGGTCCCACC TGGCATCTCT CAAGAACAGT 
GTTTCCCCTG TCTCGCGATC CCATTCCTTC AGTGACCCTT CTCCTCCCAA ATTTGCACAC 
CACCATCTTC GCTCTCAGGA CCCATGTCCA CCTTCCCGCA GTGAGGTGCT AAGTCAGAGT 
TCTGACTCTA AGTCGGAGGT ACCCGACCCC GCCCAAAAGG CTTGGTCTAG ATCAGACAGT 
GACGAGGTCC CTCCAAGGGT TCCTGTGAGA ACGACGTCCC GGTCCCCCGT CCTGTCCCGT 
CGGGATTCCC CACTGCAGGG CAGTGGGCAG CAAAACAGCC AAGCAGGTCA GAGAAACTCC 
ACCAGCAGCA TCGAGCCCAG GCTGCTATGG GAGAGAGTGG AGAAGCTGGT GCCCAGGCCT 
GGCAGTGGCA GCTCCTCGGG GTCCAGCAAC TCAGGATCCC AGCCCGGCTC CCACCCTGGG 
TCTCAGAGCG GCTCCGGGGA GCGCTTCAGA GTGAGATCGT CATCCAAATC TGAGGGCTCG 
CCATCTCAGC GCCTTGAAAA TGCAGCAAAA AAGCCTGAAG ATAAGAAGGA AGTTTTTAGA 
CCTCTCAAGC CCGCTGGCGA AGTGGACCTG ACGGCCCTGG CCAGAGAGCT CCGAGCGGCG 
GAAGACGTGC GGCCGCCGCA CAAGGTGACA GACTACTCGT CGTCCAGCGA GGAGTCCGGG 
ACAACGGATG AGGAGGACGA CGACGTGGAG CAGGAAGGCC CCGAGGAGCC CACCTCGGGG 
CCGGAGGACG CCCGAGCCGC GTCATCTCTG AACTTGAGCA ACGGTGAGAC GGAGTCCGTG 
AAAACGATGA TCGTCCACGA CGACGTGGAG AGTGAACCAG CCATGACCCC GTCCAAGGAG 
GGCACCCTGA TTGTCCGCCA GAGTGCAGTT GACCAAAAGC GCGCCAGCCA TCATGAGAGC 
AATGGCTTTG CCGGTCGCGT TCACCTCTTG CCAGATCTCT TACAGCAAAG CCATTCCTCC 
TCCACTTCCT CCACCTCCTC CTCCCCATCC TCCAGCCAGC CGACACCCAC CATGTCCCCA 
CAGACACCCC AGGACAAGCT CACTCCTAAC GAGACTCAGT CCGCTAGTAG CACACTCCAG 
AAACACAAAT CTTCCTCCTC CTTTACACCT TTTATAGACC CCAGATTACT ACAGATTTCT 
CCATCTAGTG GGACAACAGT GACTTCTGTG GTGGGATTTT CCTGTGATGG AATGAGATCG 
GAGGCCATAA GGCAAGATCC TACCCGGAAG GGCTCAGTGG TCAATGTGAA TCCCACCAAC 
ACTAGGCCAC AGAGCGACAC CCCAGAGATT CGCAAATATA AGAAGAGGTT TAACTCCGAG 
ATTCTGTGCG CTGCCTTGTG GGGAGTGAAC TTGTTGGTGG GTACAGAGAG TGGCCTGATG 
CTGCTGGACA GAAGTGGCCA AGGGAAGGTG TATCCTCTGA TCAACAGAAG ACGATTTCAA 
CAGATGGACG TCCTGGAAGG CTTGAATGTC TTGGTGACAA TATCTGGCAA AAAGGATAAG 
TTACGTGTCT ACTATTTGTC CTGGTTAAGA AATAAAATAC TTCACAACGA TCCGGAAGTT 
GAGAAGAAGC AGGGTTGGAC GACCGTGGGA GATTTGGAAG GATGCGTCCA CTATAAAGTC 
GTAAAATATG AAAGAATCAA GTTTCTGGTC ATTGCTTTGA AGAGTTCTGT GGAAGTGTAT 
GCGTGGGCAC CCAAGCCATA TCACAAATTC ATGGCCTTTA AGTCGTTTGG AGATTTGGTG 
CATAAGCCAT TACTGGTGGA TCTCACTGTT GAGGAAGGCC AGAGGTTGAA AGTGATCTAT 
GGATCCTGTG CTGGATTCCA TGCTGTTGAC GTGGATTCAG GATCAGTCTA TGACATTTAT 
CTACCAACAC ACATCCAGTG CAGCATCAAA CCCCACGCAA TCATCATCCT CCCCAACACG 
GATGGAATGG AGCTTCTGGT GTGCTATGAA GACGAAGGGG TCTATGTGAA CACCTATGGA 
AGGATTACCA AGGATGTGGT TCTGCAGTGG GGAGAGATGC CAACGTCTGT AGCGTATATT 
CGATCCAATC AGACGATGGG CTGGGGAGAA AAGGCCATAG AGATCCGGTC TGTGGAGACG 
GGCCACTTGG ACGGTGTGTT TATGCACAAA AGGGCTCAAA GACTGAAATT CTTATGTGAA 
CGCAACGACA AGGTGTTCTT TGCCTCGGTC CGGTCCGGGG GCAGCAGCCA GGTGTACTTC 
ATGACGCTGG GCAGGACCTC TCTTCTGAGC TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_019931224.1 ) cDNA ORF clone, Tursiops truncatus(common bottlenose dolphin) -> XP_019786783.1 Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OTu39183
Clone ID Related Accession (Same CDS sequence)	XM_019931224.1
Accession Version	XM_019931224.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3846bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Tursiops truncatus(common bottlenose dolphin)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017842906.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tursiops truncatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
CCGGCTGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
AAGGGTCGAC ACGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA CGTTACTGAG 
GATGAAGAGG AGGAAATCAA GCTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGCGGGGCC GGGTCCATCA CAGACCTCGT GAAGAACACC 
AAGGGCAACA CGCTCAAGGA GGACTGGATA GCCTACATCT CCAGGGAGAT CCTGAGGGGA 
CTGGCGCATC TCCACATCCA TCACGTGATT CACCGGGACA TCAAGGGCCA GAATGTATTG 
CTGACCGAGA ACGCAGAGGT GAAGCTTGTG GATTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACCGTGGGGA GGAGAAACAC CTTCATAGGC ACCCCGTACT GGATGGCTCC CGAGGTCATC 
GCCTGTGACG AGAACCCCGA CGCCACCTAT GATTACAGGA GTGACCTGTG GTCCTGCGGC 
ATCACAGCCA TTGAGATGGC AGAAGGCGCT CCCCCTCTCT GTGACATGCA TCCCATGAGA 
GCACTGTTTC TCATCCCCAG AAACCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCAAAA 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACA 
GAGCAGCTTC TGAAACATCC TTTTATCAGG GACCAGCCGA ATGAAAGGCA GGTTAGGATC 
CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACCGAG 
TACGAGTACA GCGGGAGTGA GGAGGAGGAG GAGGAGGCGC CGGAGCAGGA GGGGGAGCCC 
AGCTCCATCG TCAACGTGCC TGGCGAGTCC ACGCTGCGCC GGGACTTCCT GAGGCTGCAG 
CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGCGGCAGC AGCTCCTGCA GGAGCAGCAG 
CTCAGGGAGC AGGAAGAATA CAAGAGGCAG CTGCTGGCGG AGAGACAGAA GCGGATCGAG 
CAGCAGAAGG AGCAGCGGCG ACGGCTGGAA GAGCAACAAA GGAGAGAGCG GGAGGCCAGG 
AGGCAGCAGG AGCGCGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
CTGGAGAGAC GGCGGCAGGA GGAAGAGGAG CGCAGGCGGG CGGAGGAGGA CAAGAGGAGA 
GTCGACAGGG AGCAGGAGTA TATCAGGCGG CAGCTGGAAG AGGAGCAGCG GCACCTGGAA 
CTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCATGAGCC CCGGAGGCCG 
CCCCCGCCGC CGGACAGGAG CAAGGCCGGC CTGCACGGCG CCGAGCCCAA GGCCCAGCAC 
GAGCCCGCCG ACCGGGCCCG CGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCAGATCA 
GAATCTTTTT CCAATGGCAA CTCCGAGTCT GTGCACCCTG CCCTGCAGAG ACCAGCCGAG 
CCACAGGTAC AGTGGTCCCA CCTGGCATCT CTCAAGAACA GTGTTTCCCC TGTCTCGCGA 
TCCCATTCCT TCAGTGACCC TTCTCCTCCC AAATTTGCAC ACCACCATCT TCGCTCTCAG 
GACCCATGTC CACCTTCCCG CAGTGAGGTG CTAAGTCAGA GTTCTGACTC TAAGTCGGAG 
GTACCCGACC CCGCCCAAAA GGCTTGGTCT AGATCAGACA GTGACGAGGT CCCTCCAAGG 
GTTCCTGTGA GAACGACGTC CCGGTCCCCC GTCCTGTCCC GTCGGGATTC CCCACTGCAG 
GGCAGTGGGC AGCAAAACAG CCAAGCAGGT CAGAGAAACT CCACCAGCAG CATCGAGCCC 
AGGCTGCTAT GGGAGAGAGT GGAGAAGCTG GTGCCCAGGC CTGGCAGTGG CAGCTCCTCG 
GGGTCCAGCA ACTCAGGATC CCAGCCCGGC TCCCACCCTG GGTCTCAGAG CGGCTCCGGG 
GAGCGCTTCA GAGTGAGATC GTCATCCAAA TCTGAGGGCT CGCCATCTCA GCGCCTTGAA 
AATGCAGCAA AAAAGCCTGA AGATAAGAAG GAAGTTTTTA GACCTCTCAA GCCCGCTGGC 
GAAGTGGACC TGACGGCCCT GGCCAGAGAG CTCCGAGCGG CGGAAGACGT GCGGCCGCCG 
CACAAGGTGA CAGACTACTC GTCGTCCAGC GAGGAGTCCG GGACAACGGA TGAGGAGGAC 
GACGACGTGG AGCAGGAAGG CCCCGAGGAG CCCACCTCGG GGCCGGAGGA CGCCCGAGCC 
GCGTCATCTC TGAACTTGAG CAACGGTGAG ACGGAGTCCG TGAAAACGAT GATCGTCCAC 
GACGACGTGG AGAGTGAACC AGCCATGACC CCGTCCAAGG AGGGCACCCT GATTGTCCGC 
CAGACTCAGT CCGCTAGTAG CACACTCCAG AAACACAAAT CTTCCTCCTC CTTTACACCT 
TTTATAGACC CCAGATTACT ACAGATTTCT CCATCTAGTG GGACAACAGT GACTTCTGTG 
GTGGGATTTT CCTGTGATGG AATGAGATCG GAGGCCATAA GGCAAGATCC TACCCGGAAG 
GGCTCAGTGG TCAATGTGAA TCCCACCAAC ACTAGGCCAC AGAGCGACAC CCCAGAGATT 
CGCAAATATA AGAAGAGGTT TAACTCCGAG ATTCTGTGCG CTGCCTTGTG GGGAGTGAAC 
TTGTTGGTGG GTACAGAGAG TGGCCTGATG CTGCTGGACA GAAGTGGCCA AGGGAAGGTG 
TATCCTCTGA TCAACAGAAG ACGATTTCAA CAGATGGACG TCCTGGAAGG CTTGAATGTC 
TTGGTGACAA TATCTGGCAA AAAGGATAAG TTACGTGTCT ACTATTTGTC CTGGTTAAGA 
AATAAAATAC TTCACAACGA TCCGGAAGTT GAGAAGAAGC AGGGTTGGAC GACCGTGGGA 
GATTTGGAAG GATGCGTCCA CTATAAAGTC GTAAAATATG AAAGAATCAA GTTTCTGGTC 
ATTGCTTTGA AGAGTTCTGT GGAAGTGTAT GCGTGGGCAC CCAAGCCATA TCACAAATTC 
ATGGCCTTTA AGTCGTTTGG AGATTTGGTG CATAAGCCAT TACTGGTGGA TCTCACTGTT 
GAGGAAGGCC AGAGGTTGAA AGTGATCTAT GGATCCTGTG CTGGATTCCA TGCTGTTGAC 
GTGGATTCAG GATCAGTCTA TGACATTTAT CTACCAACAC ACATCCAGTG CAGCATCAAA 
CCCCACGCAA TCATCATCCT CCCCAACACG GATGGAATGG AGCTTCTGGT GTGCTATGAA 
GACGAAGGGG TCTATGTGAA CACCTATGGA AGGATTACCA AGGATGTGGT TCTGCAGTGG 
GGAGAGATGC CAACGTCTGT AGCGTATATT CGATCCAATC AGACGATGGG CTGGGGAGAA 
AAGGCCATAG AGATCCGGTC TGTGGAGACG GGCCACTTGG ACGGTGTGTT TATGCACAAA 
AGGGCTCAAA GACTGAAATT CTTATGTGAA CGCAACGACA AGGTGTTCTT TGCCTCGGTC 
CGGTCCGGGG GCAGCAGCCA GGTGTACTTC ATGACGCTGG GCAGGACCTC TCTTCTGAGC 
TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019786783.1
CDS	148..3993
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEAPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRQEEEERRRAEEDKRRVDREQEYIRRQLEEEQRHLELLQQQLLQEQAMLLHEPRRPPPPPDRSKAGLHGAEPKAQHEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVQWSHLASLKNSVSPVSRSHSFSDPSPPKFAHHHLRSQDPCPPSRSEVLSQSSDSKSEVPDPAQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPAGEVDLTALARELRAAEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGPEEPTSGPEDARAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRSEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGDLVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_019931224.1
Organism	Tursiops truncatus(common bottlenose dolphin)
Definition	Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019931224.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
CCGGCTGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
AAGGGTCGAC ACGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA CGTTACTGAG 
GATGAAGAGG AGGAAATCAA GCTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGCGGGGCC GGGTCCATCA CAGACCTCGT GAAGAACACC 
AAGGGCAACA CGCTCAAGGA GGACTGGATA GCCTACATCT CCAGGGAGAT CCTGAGGGGA 
CTGGCGCATC TCCACATCCA TCACGTGATT CACCGGGACA TCAAGGGCCA GAATGTATTG 
CTGACCGAGA ACGCAGAGGT GAAGCTTGTG GATTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACCGTGGGGA GGAGAAACAC CTTCATAGGC ACCCCGTACT GGATGGCTCC CGAGGTCATC 
GCCTGTGACG AGAACCCCGA CGCCACCTAT GATTACAGGA GTGACCTGTG GTCCTGCGGC 
ATCACAGCCA TTGAGATGGC AGAAGGCGCT CCCCCTCTCT GTGACATGCA TCCCATGAGA 
GCACTGTTTC TCATCCCCAG AAACCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCAAAA 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACA 
GAGCAGCTTC TGAAACATCC TTTTATCAGG GACCAGCCGA ATGAAAGGCA GGTTAGGATC 
CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACCGAG 
TACGAGTACA GCGGGAGTGA GGAGGAGGAG GAGGAGGCGC CGGAGCAGGA GGGGGAGCCC 
AGCTCCATCG TCAACGTGCC TGGCGAGTCC ACGCTGCGCC GGGACTTCCT GAGGCTGCAG 
CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGCGGCAGC AGCTCCTGCA GGAGCAGCAG 
CTCAGGGAGC AGGAAGAATA CAAGAGGCAG CTGCTGGCGG AGAGACAGAA GCGGATCGAG 
CAGCAGAAGG AGCAGCGGCG ACGGCTGGAA GAGCAACAAA GGAGAGAGCG GGAGGCCAGG 
AGGCAGCAGG AGCGCGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
CTGGAGAGAC GGCGGCAGGA GGAAGAGGAG CGCAGGCGGG CGGAGGAGGA CAAGAGGAGA 
GTCGACAGGG AGCAGGAGTA TATCAGGCGG CAGCTGGAAG AGGAGCAGCG GCACCTGGAA 
CTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCATGAGCC CCGGAGGCCG 
CCCCCGCCGC CGGACAGGAG CAAGGCCGGC CTGCACGGCG CCGAGCCCAA GGCCCAGCAC 
GAGCCCGCCG ACCGGGCCCG CGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCAGATCA 
GAATCTTTTT CCAATGGCAA CTCCGAGTCT GTGCACCCTG CCCTGCAGAG ACCAGCCGAG 
CCACAGGTAC AGTGGTCCCA CCTGGCATCT CTCAAGAACA GTGTTTCCCC TGTCTCGCGA 
TCCCATTCCT TCAGTGACCC TTCTCCTCCC AAATTTGCAC ACCACCATCT TCGCTCTCAG 
GACCCATGTC CACCTTCCCG CAGTGAGGTG CTAAGTCAGA GTTCTGACTC TAAGTCGGAG 
GTACCCGACC CCGCCCAAAA GGCTTGGTCT AGATCAGACA GTGACGAGGT CCCTCCAAGG 
GTTCCTGTGA GAACGACGTC CCGGTCCCCC GTCCTGTCCC GTCGGGATTC CCCACTGCAG 
GGCAGTGGGC AGCAAAACAG CCAAGCAGGT CAGAGAAACT CCACCAGCAG CATCGAGCCC 
AGGCTGCTAT GGGAGAGAGT GGAGAAGCTG GTGCCCAGGC CTGGCAGTGG CAGCTCCTCG 
GGGTCCAGCA ACTCAGGATC CCAGCCCGGC TCCCACCCTG GGTCTCAGAG CGGCTCCGGG 
GAGCGCTTCA GAGTGAGATC GTCATCCAAA TCTGAGGGCT CGCCATCTCA GCGCCTTGAA 
AATGCAGCAA AAAAGCCTGA AGATAAGAAG GAAGTTTTTA GACCTCTCAA GCCCGCTGGC 
GAAGTGGACC TGACGGCCCT GGCCAGAGAG CTCCGAGCGG CGGAAGACGT GCGGCCGCCG 
CACAAGGTGA CAGACTACTC GTCGTCCAGC GAGGAGTCCG GGACAACGGA TGAGGAGGAC 
GACGACGTGG AGCAGGAAGG CCCCGAGGAG CCCACCTCGG GGCCGGAGGA CGCCCGAGCC 
GCGTCATCTC TGAACTTGAG CAACGGTGAG ACGGAGTCCG TGAAAACGAT GATCGTCCAC 
GACGACGTGG AGAGTGAACC AGCCATGACC CCGTCCAAGG AGGGCACCCT GATTGTCCGC 
CAGACTCAGT CCGCTAGTAG CACACTCCAG AAACACAAAT CTTCCTCCTC CTTTACACCT 
TTTATAGACC CCAGATTACT ACAGATTTCT CCATCTAGTG GGACAACAGT GACTTCTGTG 
GTGGGATTTT CCTGTGATGG AATGAGATCG GAGGCCATAA GGCAAGATCC TACCCGGAAG 
GGCTCAGTGG TCAATGTGAA TCCCACCAAC ACTAGGCCAC AGAGCGACAC CCCAGAGATT 
CGCAAATATA AGAAGAGGTT TAACTCCGAG ATTCTGTGCG CTGCCTTGTG GGGAGTGAAC 
TTGTTGGTGG GTACAGAGAG TGGCCTGATG CTGCTGGACA GAAGTGGCCA AGGGAAGGTG 
TATCCTCTGA TCAACAGAAG ACGATTTCAA CAGATGGACG TCCTGGAAGG CTTGAATGTC 
TTGGTGACAA TATCTGGCAA AAAGGATAAG TTACGTGTCT ACTATTTGTC CTGGTTAAGA 
AATAAAATAC TTCACAACGA TCCGGAAGTT GAGAAGAAGC AGGGTTGGAC GACCGTGGGA 
GATTTGGAAG GATGCGTCCA CTATAAAGTC GTAAAATATG AAAGAATCAA GTTTCTGGTC 
ATTGCTTTGA AGAGTTCTGT GGAAGTGTAT GCGTGGGCAC CCAAGCCATA TCACAAATTC 
ATGGCCTTTA AGTCGTTTGG AGATTTGGTG CATAAGCCAT TACTGGTGGA TCTCACTGTT 
GAGGAAGGCC AGAGGTTGAA AGTGATCTAT GGATCCTGTG CTGGATTCCA TGCTGTTGAC 
GTGGATTCAG GATCAGTCTA TGACATTTAT CTACCAACAC ACATCCAGTG CAGCATCAAA 
CCCCACGCAA TCATCATCCT CCCCAACACG GATGGAATGG AGCTTCTGGT GTGCTATGAA 
GACGAAGGGG TCTATGTGAA CACCTATGGA AGGATTACCA AGGATGTGGT TCTGCAGTGG 
GGAGAGATGC CAACGTCTGT AGCGTATATT CGATCCAATC AGACGATGGG CTGGGGAGAA 
AAGGCCATAG AGATCCGGTC TGTGGAGACG GGCCACTTGG ACGGTGTGTT TATGCACAAA 
AGGGCTCAAA GACTGAAATT CTTATGTGAA CGCAACGACA AGGTGTTCTT TGCCTCGGTC 
CGGTCCGGGG GCAGCAGCCA GGTGTACTTC ATGACGCTGG GCAGGACCTC TCTTCTGAGC 
TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_019931225.1 ) cDNA ORF clone, Tursiops truncatus(common bottlenose dolphin) -> XP_019786784.1 Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OTu39184
Clone ID Related Accession (Same CDS sequence)	XM_019931225.1
Accession Version	XM_019931225.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Tursiops truncatus(common bottlenose dolphin)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017842906.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tursiops truncatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
CCGGCTGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
AAGGGTCGAC ACGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA CGTTACTGAG 
GATGAAGAGG AGGAAATCAA GCTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGCGGGGCC GGGTCCATCA CAGACCTCGT GAAGAACACC 
AAGGGCAACA CGCTCAAGGA GGACTGGATA GCCTACATCT CCAGGGAGAT CCTGAGGGGA 
CTGGCGCATC TCCACATCCA TCACGTGATT CACCGGGACA TCAAGGGCCA GAATGTATTG 
CTGACCGAGA ACGCAGAGGT GAAGCTTGTG GATTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACCGTGGGGA GGAGAAACAC CTTCATAGGC ACCCCGTACT GGATGGCTCC CGAGGTCATC 
GCCTGTGACG AGAACCCCGA CGCCACCTAT GATTACAGGA GTGACCTGTG GTCCTGCGGC 
ATCACAGCCA TTGAGATGGC AGAAGGCGCT CCCCCTCTCT GTGACATGCA TCCCATGAGA 
GCACTGTTTC TCATCCCCAG AAACCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCAAAA 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACA 
GAGCAGCTTC TGAAACATCC TTTTATCAGG GACCAGCCGA ATGAAAGGCA GGTTAGGATC 
CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACCGAG 
TACGAGTACA GCGGGAGTGA GGAGGAGGAG GAGGAGGCGC CGGAGCAGGA GGGGGAGCCC 
AGCTCCATCG TCAACGTGCC TGGCGAGTCC ACGCTGCGCC GGGACTTCCT GAGGCTGCAG 
CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGCGGCAGC AGCTCCTGCA GGAGCAGCAG 
CTCAGGGAGC AGGAAGAATA CAAGAGGCAG CTGCTGGCGG AGAGACAGAA GCGGATCGAG 
CAGCAGAAGG AGCAGCGGCG ACGGCTGGAA GAGCAACAAA GGAGAGAGCG GGAGGCCAGG 
AGGCAGCAGG AGCGCGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
CTGGAGAGAC GGCGGCAGGA GGAAGAGGAG CGCAGGCGGG CGGAGGAGGA CAAGAGGAGA 
GTCGACAGGG AGCAGGAGTA TATCAGGCGG CAGCTGGAAG AGGAGCAGCG GCACCTGGAA 
CTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCATGAGCC CCGGAGGCCG 
CCCCCGCCGC CGGACAGGAG CAAGGCCGGC CTGCACGGCG CCGAGCCCAA GGCCCAGCAC 
GAGCCCGCCG ACCGGGCCCG CGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCAGATCA 
GAATCTTTTT CCAATGGCAA CTCCGAGTCT GTGCACCCTG CCCTGCAGAG ACCAGCCGAG 
CCACAGGTTC CTGTGAGAAC GACGTCCCGG TCCCCCGTCC TGTCCCGTCG GGATTCCCCA 
CTGCAGGGCA GTGGGCAGCA AAACAGCCAA GCAGGTCAGA GAAACTCCAC CAGCAGCATC 
GAGCCCAGGC TGCTATGGGA GAGAGTGGAG AAGCTGGTGC CCAGGCCTGG CAGTGGCAGC 
TCCTCGGGGT CCAGCAACTC AGGATCCCAG CCCGGCTCCC ACCCTGGGTC TCAGAGCGGC 
TCCGGGGAGC GCTTCAGAGT GAGATCGTCA TCCAAATCTG AGGGCTCGCC ATCTCAGCGC 
CTTGAAAATG CAGCAAAAAA GCCTGAAGAT AAGAAGGAAG TTTTTAGACC TCTCAAGCCC 
GCTGACCTGA CGGCCCTGGC CAGAGAGCTC CGAGCGGCGG AAGACGTGCG GCCGCCGCAC 
AAGGTGACAG ACTACTCGTC GTCCAGCGAG GAGTCCGGGA CAACGGATGA GGAGGACGAC 
GACGTGGAGC AGGAAGGCCC CGAGGAGCCC ACCTCGGGGC CGGAGGACGC CCGAGCCGCG 
TCATCTCTGA ACTTGAGCAA CGGTGAGACG GAGTCCGTGA AAACGATGAT CGTCCACGAC 
GACGTGGAGA GTGAACCAGC CATGACCCCG TCCAAGGAGG GCACCCTGAT TGTCCGCCAG 
AGTGCAGTTG ACCAAAAGCG CGCCAGCCAT CATGAGAGCA ATGGCTTTGC CGGTCGCGTT 
CACCTCTTGC CAGATCTCTT ACAGCAAAGC CATTCCTCCT CCACTTCCTC CACCTCCTCC 
TCCCCATCCT CCAGCCAGCC GACACCCACC ATGTCCCCAC AGACACCCCA GGACAAGCTC 
ACTCCTAACG AGACTCAGTC CGCTAGTAGC ACACTCCAGA AACACAAATC TTCCTCCTCC 
TTTACACCTT TTATAGACCC CAGATTACTA CAGATTTCTC CATCTAGTGG GACAACAGTG 
ACTTCTGTGG TGGGATTTTC CTGTGATGGA ATGAGATCGG AGGCCATAAG GCAAGATCCT 
ACCCGGAAGG GCTCAGTGGT CAATGTGAAT CCCACCAACA CTAGGCCACA GAGCGACACC 
CCAGAGATTC GCAAATATAA GAAGAGGTTT AACTCCGAGA TTCTGTGCGC TGCCTTGTGG 
GGAGTGAACT TGTTGGTGGG TACAGAGAGT GGCCTGATGC TGCTGGACAG AAGTGGCCAA 
GGGAAGGTGT ATCCTCTGAT CAACAGAAGA CGATTTCAAC AGATGGACGT CCTGGAAGGC 
TTGAATGTCT TGGTGACAAT ATCTGGCAAA AAGGATAAGT TACGTGTCTA CTATTTGTCC 
TGGTTAAGAA ATAAAATACT TCACAACGAT CCGGAAGTTG AGAAGAAGCA GGGTTGGACG 
ACCGTGGGAG ATTTGGAAGG ATGCGTCCAC TATAAAGTCG TAAAATATGA AAGAATCAAG 
TTTCTGGTCA TTGCTTTGAA GAGTTCTGTG GAAGTGTATG CGTGGGCACC CAAGCCATAT 
CACAAATTCA TGGCCTTTAA GTCGTTTGGA GATTTGGTGC ATAAGCCATT ACTGGTGGAT 
CTCACTGTTG AGGAAGGCCA GAGGTTGAAA GTGATCTATG GATCCTGTGC TGGATTCCAT 
GCTGTTGACG TGGATTCAGG ATCAGTCTAT GACATTTATC TACCAACACA CATCCAGTGC 
AGCATCAAAC CCCACGCAAT CATCATCCTC CCCAACACGG ATGGAATGGA GCTTCTGGTG 
TGCTATGAAG ACGAAGGGGT CTATGTGAAC ACCTATGGAA GGATTACCAA GGATGTGGTT 
CTGCAGTGGG GAGAGATGCC AACGTCTGTA GCGTATATTC GATCCAATCA GACGATGGGC 
TGGGGAGAAA AGGCCATAGA GATCCGGTCT GTGGAGACGG GCCACTTGGA CGGTGTGTTT 
ATGCACAAAA GGGCTCAAAG ACTGAAATTC TTATGTGAAC GCAACGACAA GGTGTTCTTT 
GCCTCGGTCC GGTCCGGGGG CAGCAGCCAG GTGTACTTCA TGACGCTGGG CAGGACCTCT 
CTTCTGAGCT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019786784.1
CDS	147..3941
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEAPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRQEEEERRRAEEDKRRVDREQEYIRRQLEEEQRHLELLQQQLLQEQAMLLHEPRRPPPPPDRSKAGLHGAEPKAQHEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPADLTALARELRAAEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGPEEPTSGPEDARAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSAVDQKRASHHESNGFAGRVHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTPNETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRSEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGDLVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_019931225.1
Organism	Tursiops truncatus(common bottlenose dolphin)
Definition	Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019931225.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
CCGGCTGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
AAGGGTCGAC ACGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA CGTTACTGAG 
GATGAAGAGG AGGAAATCAA GCTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGCGGGGCC GGGTCCATCA CAGACCTCGT GAAGAACACC 
AAGGGCAACA CGCTCAAGGA GGACTGGATA GCCTACATCT CCAGGGAGAT CCTGAGGGGA 
CTGGCGCATC TCCACATCCA TCACGTGATT CACCGGGACA TCAAGGGCCA GAATGTATTG 
CTGACCGAGA ACGCAGAGGT GAAGCTTGTG GATTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACCGTGGGGA GGAGAAACAC CTTCATAGGC ACCCCGTACT GGATGGCTCC CGAGGTCATC 
GCCTGTGACG AGAACCCCGA CGCCACCTAT GATTACAGGA GTGACCTGTG GTCCTGCGGC 
ATCACAGCCA TTGAGATGGC AGAAGGCGCT CCCCCTCTCT GTGACATGCA TCCCATGAGA 
GCACTGTTTC TCATCCCCAG AAACCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCAAAA 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACA 
GAGCAGCTTC TGAAACATCC TTTTATCAGG GACCAGCCGA ATGAAAGGCA GGTTAGGATC 
CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACCGAG 
TACGAGTACA GCGGGAGTGA GGAGGAGGAG GAGGAGGCGC CGGAGCAGGA GGGGGAGCCC 
AGCTCCATCG TCAACGTGCC TGGCGAGTCC ACGCTGCGCC GGGACTTCCT GAGGCTGCAG 
CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGCGGCAGC AGCTCCTGCA GGAGCAGCAG 
CTCAGGGAGC AGGAAGAATA CAAGAGGCAG CTGCTGGCGG AGAGACAGAA GCGGATCGAG 
CAGCAGAAGG AGCAGCGGCG ACGGCTGGAA GAGCAACAAA GGAGAGAGCG GGAGGCCAGG 
AGGCAGCAGG AGCGCGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
CTGGAGAGAC GGCGGCAGGA GGAAGAGGAG CGCAGGCGGG CGGAGGAGGA CAAGAGGAGA 
GTCGACAGGG AGCAGGAGTA TATCAGGCGG CAGCTGGAAG AGGAGCAGCG GCACCTGGAA 
CTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCATGAGCC CCGGAGGCCG 
CCCCCGCCGC CGGACAGGAG CAAGGCCGGC CTGCACGGCG CCGAGCCCAA GGCCCAGCAC 
GAGCCCGCCG ACCGGGCCCG CGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCAGATCA 
GAATCTTTTT CCAATGGCAA CTCCGAGTCT GTGCACCCTG CCCTGCAGAG ACCAGCCGAG 
CCACAGGTTC CTGTGAGAAC GACGTCCCGG TCCCCCGTCC TGTCCCGTCG GGATTCCCCA 
CTGCAGGGCA GTGGGCAGCA AAACAGCCAA GCAGGTCAGA GAAACTCCAC CAGCAGCATC 
GAGCCCAGGC TGCTATGGGA GAGAGTGGAG AAGCTGGTGC CCAGGCCTGG CAGTGGCAGC 
TCCTCGGGGT CCAGCAACTC AGGATCCCAG CCCGGCTCCC ACCCTGGGTC TCAGAGCGGC 
TCCGGGGAGC GCTTCAGAGT GAGATCGTCA TCCAAATCTG AGGGCTCGCC ATCTCAGCGC 
CTTGAAAATG CAGCAAAAAA GCCTGAAGAT AAGAAGGAAG TTTTTAGACC TCTCAAGCCC 
GCTGACCTGA CGGCCCTGGC CAGAGAGCTC CGAGCGGCGG AAGACGTGCG GCCGCCGCAC 
AAGGTGACAG ACTACTCGTC GTCCAGCGAG GAGTCCGGGA CAACGGATGA GGAGGACGAC 
GACGTGGAGC AGGAAGGCCC CGAGGAGCCC ACCTCGGGGC CGGAGGACGC CCGAGCCGCG 
TCATCTCTGA ACTTGAGCAA CGGTGAGACG GAGTCCGTGA AAACGATGAT CGTCCACGAC 
GACGTGGAGA GTGAACCAGC CATGACCCCG TCCAAGGAGG GCACCCTGAT TGTCCGCCAG 
AGTGCAGTTG ACCAAAAGCG CGCCAGCCAT CATGAGAGCA ATGGCTTTGC CGGTCGCGTT 
CACCTCTTGC CAGATCTCTT ACAGCAAAGC CATTCCTCCT CCACTTCCTC CACCTCCTCC 
TCCCCATCCT CCAGCCAGCC GACACCCACC ATGTCCCCAC AGACACCCCA GGACAAGCTC 
ACTCCTAACG AGACTCAGTC CGCTAGTAGC ACACTCCAGA AACACAAATC TTCCTCCTCC 
TTTACACCTT TTATAGACCC CAGATTACTA CAGATTTCTC CATCTAGTGG GACAACAGTG 
ACTTCTGTGG TGGGATTTTC CTGTGATGGA ATGAGATCGG AGGCCATAAG GCAAGATCCT 
ACCCGGAAGG GCTCAGTGGT CAATGTGAAT CCCACCAACA CTAGGCCACA GAGCGACACC 
CCAGAGATTC GCAAATATAA GAAGAGGTTT AACTCCGAGA TTCTGTGCGC TGCCTTGTGG 
GGAGTGAACT TGTTGGTGGG TACAGAGAGT GGCCTGATGC TGCTGGACAG AAGTGGCCAA 
GGGAAGGTGT ATCCTCTGAT CAACAGAAGA CGATTTCAAC AGATGGACGT CCTGGAAGGC 
TTGAATGTCT TGGTGACAAT ATCTGGCAAA AAGGATAAGT TACGTGTCTA CTATTTGTCC 
TGGTTAAGAA ATAAAATACT TCACAACGAT CCGGAAGTTG AGAAGAAGCA GGGTTGGACG 
ACCGTGGGAG ATTTGGAAGG ATGCGTCCAC TATAAAGTCG TAAAATATGA AAGAATCAAG 
TTTCTGGTCA TTGCTTTGAA GAGTTCTGTG GAAGTGTATG CGTGGGCACC CAAGCCATAT 
CACAAATTCA TGGCCTTTAA GTCGTTTGGA GATTTGGTGC ATAAGCCATT ACTGGTGGAT 
CTCACTGTTG AGGAAGGCCA GAGGTTGAAA GTGATCTATG GATCCTGTGC TGGATTCCAT 
GCTGTTGACG TGGATTCAGG ATCAGTCTAT GACATTTATC TACCAACACA CATCCAGTGC 
AGCATCAAAC CCCACGCAAT CATCATCCTC CCCAACACGG ATGGAATGGA GCTTCTGGTG 
TGCTATGAAG ACGAAGGGGT CTATGTGAAC ACCTATGGAA GGATTACCAA GGATGTGGTT 
CTGCAGTGGG GAGAGATGCC AACGTCTGTA GCGTATATTC GATCCAATCA GACGATGGGC 
TGGGGAGAAA AGGCCATAGA GATCCGGTCT GTGGAGACGG GCCACTTGGA CGGTGTGTTT 
ATGCACAAAA GGGCTCAAAG ACTGAAATTC TTATGTGAAC GCAACGACAA GGTGTTCTTT 
GCCTCGGTCC GGTCCGGGGG CAGCAGCCAG GTGTACTTCA TGACGCTGGG CAGGACCTCT 
CTTCTGAGCT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_019931226.1 ) cDNA ORF clone, Tursiops truncatus(common bottlenose dolphin) -> XP_019786785.1 Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X9, mRNA.

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CloneID	OTu39185
Clone ID Related Accession (Same CDS sequence)	XM_019931226.1
Accession Version	XM_019931226.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Tursiops truncatus(common bottlenose dolphin)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017842906.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tursiops truncatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
CCGGCTGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
AAGGGTCGAC ACGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA CGTTACTGAG 
GATGAAGAGG AGGAAATCAA GCTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGCGGGGCC GGGTCCATCA CAGACCTCGT GAAGAACACC 
AAGGGCAACA CGCTCAAGGA GGACTGGATA GCCTACATCT CCAGGGAGAT CCTGAGGGGA 
CTGGCGCATC TCCACATCCA TCACGTGATT CACCGGGACA TCAAGGGCCA GAATGTATTG 
CTGACCGAGA ACGCAGAGGT GAAGCTTGTG GATTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACCGTGGGGA GGAGAAACAC CTTCATAGGC ACCCCGTACT GGATGGCTCC CGAGGTCATC 
GCCTGTGACG AGAACCCCGA CGCCACCTAT GATTACAGGA GTGACCTGTG GTCCTGCGGC 
ATCACAGCCA TTGAGATGGC AGAAGGCGCT CCCCCTCTCT GTGACATGCA TCCCATGAGA 
GCACTGTTTC TCATCCCCAG AAACCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCAAAA 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACA 
GAGCAGCTTC TGAAACATCC TTTTATCAGG GACCAGCCGA ATGAAAGGCA GGTTAGGATC 
CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACCGAG 
TACGAGTACA GCGGGAGTGA GGAGGAGGAG GAGGAGGCGC CGGAGCAGGA GGGGGAGCCC 
AGCTCCATCG TCAACGTGCC TGGCGAGTCC ACGCTGCGCC GGGACTTCCT GAGGCTGCAG 
CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGCGGCAGC AGCTCCTGCA GGAGCAGCAG 
CTCAGGGAGC AGGAAGAATA CAAGAGGCAG CTGCTGGCGG AGAGACAGAA GCGGATCGAG 
CAGCAGAAGG AGCAGCGGCG ACGGCTGGAA GAGCAACAAA GGAGAGAGCG GGAGGCCAGG 
AGGCAGCAGG AGCGCGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
CTGGAGAGAC GGCGGCAGGA GGAAGAGGAG CGCAGGCGGG CGGAGGAGGA CAAGAGGAGA 
GTCGACAGGG AGCAGGAGTA TATCAGGCGG CAGCTGGAAG AGGAGCAGCG GCACCTGGAA 
CTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
CTGGAGGAGC AGCGGCAGAC GGAGCGGCTC CAGAGGCAGC TGCAGCAGGA GCAAGCCTAT 
CTCCTGTCTC TGCAGCATGA GCCCCGGAGG CCGCCCCCGC CGCCGGACAG GAGCAAGGCC 
GGCCTGCACG GCGCCGAGCC CAAGGCCCAG CACGAGCCCG CCGACCGGGC CCGCGAGGTT 
CCTGTGAGAA CGACGTCCCG GTCCCCCGTC CTGTCCCGTC GGGATTCCCC ACTGCAGGGC 
AGTGGGCAGC AAAACAGCCA AGCAGGTCAG AGAAACTCCA CCAGCAGCAT CGAGCCCAGG 
CTGCTATGGG AGAGAGTGGA GAAGCTGGTG CCCAGGCCTG GCAGTGGCAG CTCCTCGGGG 
TCCAGCAACT CAGGATCCCA GCCCGGCTCC CACCCTGGGT CTCAGAGCGG CTCCGGGGAG 
CGCTTCAGAG TGAGATCGTC ATCCAAATCT GAGGGCTCGC CATCTCAGCG CCTTGAAAAT 
GCAGCAAAAA AGCCTGAAGA TAAGAAGGAA GTTTTTAGAC CTCTCAAGCC CGCTGGCGAA 
GTGGACCTGA CGGCCCTGGC CAGAGAGCTC CGAGCGGCGG AAGACGTGCG GCCGCCGCAC 
AAGGTGACAG ACTACTCGTC GTCCAGCGAG GAGTCCGGGA CAACGGATGA GGAGGACGAC 
GACGTGGAGC AGGAAGGCCC CGAGGAGCCC ACCTCGGGGC CGGAGGACGC CCGAGCCGCG 
TCATCTCTGA ACTTGAGCAA CGGTGAGACG GAGTCCGTGA AAACGATGAT CGTCCACGAC 
GACGTGGAGA GTGAACCAGC CATGACCCCG TCCAAGGAGG GCACCCTGAT TGTCCGCCAG 
AGTGCAGTTG ACCAAAAGCG CGCCAGCCAT CATGAGAGCA ATGGCTTTGC CGGTCGCGTT 
CACCTCTTGC CAGATCTCTT ACAGCAAAGC CATTCCTCCT CCACTTCCTC CACCTCCTCC 
TCCCCATCCT CCAGCCAGCC GACACCCACC ATGTCCCCAC AGACACCCCA GGACAAGCTC 
ACTCCTAACG AGACTCAGTC CGCTAGTAGC ACACTCCAGA AACACAAATC TTCCTCCTCC 
TTTACACCTT TTATAGACCC CAGATTACTA CAGATTTCTC CATCTAGTGG GACAACAGTG 
ACTTCTGTGG TGGGATTTTC CTGTGATGGA ATGAGATCGG AGGCCATAAG GCAAGATCCT 
ACCCGGAAGG GCTCAGTGGT CAATGTGAAT CCCACCAACA CTAGGCCACA GAGCGACACC 
CCAGAGATTC GCAAATATAA GAAGAGGTTT AACTCCGAGA TTCTGTGCGC TGCCTTGTGG 
GGAGTGAACT TGTTGGTGGG TACAGAGAGT GGCCTGATGC TGCTGGACAG AAGTGGCCAA 
GGGAAGGTGT ATCCTCTGAT CAACAGAAGA CGATTTCAAC AGATGGACGT CCTGGAAGGC 
TTGAATGTCT TGGTGACAAT ATCTGGCAAA AAGGATAAGT TACGTGTCTA CTATTTGTCC 
TGGTTAAGAA ATAAAATACT TCACAACGAT CCGGAAGTTG AGAAGAAGCA GGGTTGGACG 
ACCGTGGGAG ATTTGGAAGG ATGCGTCCAC TATAAAGTCG TAAAATATGA AAGAATCAAG 
TTTCTGGTCA TTGCTTTGAA GAGTTCTGTG GAAGTGTATG CGTGGGCACC CAAGCCATAT 
CACAAATTCA TGGCCTTTAA GTCGTTTGGA GATTTGGTGC ATAAGCCATT ACTGGTGGAT 
CTCACTGTTG AGGAAGGCCA GAGGTTGAAA GTGATCTATG GATCCTGTGC TGGATTCCAT 
GCTGTTGACG TGGATTCAGG ATCAGTCTAT GACATTTATC TACCAACACA CGTAAGAAAG 
AACCCACACT CTATGATCCA GTGCAGCATC AAACCCCACG CAATCATCAT CCTCCCCAAC 
ACGGATGGAA TGGAGCTTCT GGTGTGCTAT GAAGACGAAG GGGTCTATGT GAACACCTAT 
GGAAGGATTA CCAAGGATGT GGTTCTGCAG TGGGGAGAGA TGCCAACGTC TGTAGCGTAT 
ATTCGATCCA ATCAGACGAT GGGCTGGGGA GAAAAGGCCA TAGAGATCCG GTCTGTGGAG 
ACGGGCCACT TGGACGGTGT GTTTATGCAC AAAAGGGCTC AAAGACTGAA ATTCTTATGT 
GAACGCAACG ACAAGGTGTT CTTTGCCTCG GTCCGGTCCG GGGGCAGCAG CCAGGTGTAC 
TTCATGACGC TGGGCAGGAC CTCTCTTCTG AGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019786785.1
CDS	147..3905
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEAPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRQEEEERRRAEEDKRRVDREQEYIRRQLEEEQRHLELLQQQLLQEQAMLLECRWRELEEQRQTERLQRQLQQEQAYLLSLQHEPRRPPPPPDRSKAGLHGAEPKAQHEPADRAREVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPAGEVDLTALARELRAAEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGPEEPTSGPEDARAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSAVDQKRASHHESNGFAGRVHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTPNETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRSEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGDLVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_019931226.1
Organism	Tursiops truncatus(common bottlenose dolphin)
Definition	Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019931226.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
CCGGCTGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
AAGGGTCGAC ACGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA CGTTACTGAG 
GATGAAGAGG AGGAAATCAA GCTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGCGGGGCC GGGTCCATCA CAGACCTCGT GAAGAACACC 
AAGGGCAACA CGCTCAAGGA GGACTGGATA GCCTACATCT CCAGGGAGAT CCTGAGGGGA 
CTGGCGCATC TCCACATCCA TCACGTGATT CACCGGGACA TCAAGGGCCA GAATGTATTG 
CTGACCGAGA ACGCAGAGGT GAAGCTTGTG GATTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACCGTGGGGA GGAGAAACAC CTTCATAGGC ACCCCGTACT GGATGGCTCC CGAGGTCATC 
GCCTGTGACG AGAACCCCGA CGCCACCTAT GATTACAGGA GTGACCTGTG GTCCTGCGGC 
ATCACAGCCA TTGAGATGGC AGAAGGCGCT CCCCCTCTCT GTGACATGCA TCCCATGAGA 
GCACTGTTTC TCATCCCCAG AAACCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCAAAA 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACA 
GAGCAGCTTC TGAAACATCC TTTTATCAGG GACCAGCCGA ATGAAAGGCA GGTTAGGATC 
CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACCGAG 
TACGAGTACA GCGGGAGTGA GGAGGAGGAG GAGGAGGCGC CGGAGCAGGA GGGGGAGCCC 
AGCTCCATCG TCAACGTGCC TGGCGAGTCC ACGCTGCGCC GGGACTTCCT GAGGCTGCAG 
CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGCGGCAGC AGCTCCTGCA GGAGCAGCAG 
CTCAGGGAGC AGGAAGAATA CAAGAGGCAG CTGCTGGCGG AGAGACAGAA GCGGATCGAG 
CAGCAGAAGG AGCAGCGGCG ACGGCTGGAA GAGCAACAAA GGAGAGAGCG GGAGGCCAGG 
AGGCAGCAGG AGCGCGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
CTGGAGAGAC GGCGGCAGGA GGAAGAGGAG CGCAGGCGGG CGGAGGAGGA CAAGAGGAGA 
GTCGACAGGG AGCAGGAGTA TATCAGGCGG CAGCTGGAAG AGGAGCAGCG GCACCTGGAA 
CTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
CTGGAGGAGC AGCGGCAGAC GGAGCGGCTC CAGAGGCAGC TGCAGCAGGA GCAAGCCTAT 
CTCCTGTCTC TGCAGCATGA GCCCCGGAGG CCGCCCCCGC CGCCGGACAG GAGCAAGGCC 
GGCCTGCACG GCGCCGAGCC CAAGGCCCAG CACGAGCCCG CCGACCGGGC CCGCGAGGTT 
CCTGTGAGAA CGACGTCCCG GTCCCCCGTC CTGTCCCGTC GGGATTCCCC ACTGCAGGGC 
AGTGGGCAGC AAAACAGCCA AGCAGGTCAG AGAAACTCCA CCAGCAGCAT CGAGCCCAGG 
CTGCTATGGG AGAGAGTGGA GAAGCTGGTG CCCAGGCCTG GCAGTGGCAG CTCCTCGGGG 
TCCAGCAACT CAGGATCCCA GCCCGGCTCC CACCCTGGGT CTCAGAGCGG CTCCGGGGAG 
CGCTTCAGAG TGAGATCGTC ATCCAAATCT GAGGGCTCGC CATCTCAGCG CCTTGAAAAT 
GCAGCAAAAA AGCCTGAAGA TAAGAAGGAA GTTTTTAGAC CTCTCAAGCC CGCTGGCGAA 
GTGGACCTGA CGGCCCTGGC CAGAGAGCTC CGAGCGGCGG AAGACGTGCG GCCGCCGCAC 
AAGGTGACAG ACTACTCGTC GTCCAGCGAG GAGTCCGGGA CAACGGATGA GGAGGACGAC 
GACGTGGAGC AGGAAGGCCC CGAGGAGCCC ACCTCGGGGC CGGAGGACGC CCGAGCCGCG 
TCATCTCTGA ACTTGAGCAA CGGTGAGACG GAGTCCGTGA AAACGATGAT CGTCCACGAC 
GACGTGGAGA GTGAACCAGC CATGACCCCG TCCAAGGAGG GCACCCTGAT TGTCCGCCAG 
AGTGCAGTTG ACCAAAAGCG CGCCAGCCAT CATGAGAGCA ATGGCTTTGC CGGTCGCGTT 
CACCTCTTGC CAGATCTCTT ACAGCAAAGC CATTCCTCCT CCACTTCCTC CACCTCCTCC 
TCCCCATCCT CCAGCCAGCC GACACCCACC ATGTCCCCAC AGACACCCCA GGACAAGCTC 
ACTCCTAACG AGACTCAGTC CGCTAGTAGC ACACTCCAGA AACACAAATC TTCCTCCTCC 
TTTACACCTT TTATAGACCC CAGATTACTA CAGATTTCTC CATCTAGTGG GACAACAGTG 
ACTTCTGTGG TGGGATTTTC CTGTGATGGA ATGAGATCGG AGGCCATAAG GCAAGATCCT 
ACCCGGAAGG GCTCAGTGGT CAATGTGAAT CCCACCAACA CTAGGCCACA GAGCGACACC 
CCAGAGATTC GCAAATATAA GAAGAGGTTT AACTCCGAGA TTCTGTGCGC TGCCTTGTGG 
GGAGTGAACT TGTTGGTGGG TACAGAGAGT GGCCTGATGC TGCTGGACAG AAGTGGCCAA 
GGGAAGGTGT ATCCTCTGAT CAACAGAAGA CGATTTCAAC AGATGGACGT CCTGGAAGGC 
TTGAATGTCT TGGTGACAAT ATCTGGCAAA AAGGATAAGT TACGTGTCTA CTATTTGTCC 
TGGTTAAGAA ATAAAATACT TCACAACGAT CCGGAAGTTG AGAAGAAGCA GGGTTGGACG 
ACCGTGGGAG ATTTGGAAGG ATGCGTCCAC TATAAAGTCG TAAAATATGA AAGAATCAAG 
TTTCTGGTCA TTGCTTTGAA GAGTTCTGTG GAAGTGTATG CGTGGGCACC CAAGCCATAT 
CACAAATTCA TGGCCTTTAA GTCGTTTGGA GATTTGGTGC ATAAGCCATT ACTGGTGGAT 
CTCACTGTTG AGGAAGGCCA GAGGTTGAAA GTGATCTATG GATCCTGTGC TGGATTCCAT 
GCTGTTGACG TGGATTCAGG ATCAGTCTAT GACATTTATC TACCAACACA CGTAAGAAAG 
AACCCACACT CTATGATCCA GTGCAGCATC AAACCCCACG CAATCATCAT CCTCCCCAAC 
ACGGATGGAA TGGAGCTTCT GGTGTGCTAT GAAGACGAAG GGGTCTATGT GAACACCTAT 
GGAAGGATTA CCAAGGATGT GGTTCTGCAG TGGGGAGAGA TGCCAACGTC TGTAGCGTAT 
ATTCGATCCA ATCAGACGAT GGGCTGGGGA GAAAAGGCCA TAGAGATCCG GTCTGTGGAG 
ACGGGCCACT TGGACGGTGT GTTTATGCAC AAAAGGGCTC AAAGACTGAA ATTCTTATGT 
GAACGCAACG ACAAGGTGTT CTTTGCCTCG GTCCGGTCCG GGGGCAGCAG CCAGGTGTAC 
TTCATGACGC TGGGCAGGAC CTCTCTTCTG AGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_019931227.1 ) cDNA ORF clone, Tursiops truncatus(common bottlenose dolphin) -> XP_019786786.1 Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OTu39186
Clone ID Related Accession (Same CDS sequence)	XM_019931227.1
Accession Version	XM_019931227.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Tursiops truncatus(common bottlenose dolphin)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017842906.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tursiops truncatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
CCGGCTGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
AAGGGTCGAC ACGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA CGTTACTGAG 
GATGAAGAGG AGGAAATCAA GCTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGCGGGGCC GGGTCCATCA CAGACCTCGT GAAGAACACC 
AAGGGCAACA CGCTCAAGGA GGACTGGATA GCCTACATCT CCAGGGAGAT CCTGAGGGGA 
CTGGCGCATC TCCACATCCA TCACGTGATT CACCGGGACA TCAAGGGCCA GAATGTATTG 
CTGACCGAGA ACGCAGAGGT GAAGCTTGTG GATTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACCGTGGGGA GGAGAAACAC CTTCATAGGC ACCCCGTACT GGATGGCTCC CGAGGTCATC 
GCCTGTGACG AGAACCCCGA CGCCACCTAT GATTACAGGA GTGACCTGTG GTCCTGCGGC 
ATCACAGCCA TTGAGATGGC AGAAGGCGCT CCCCCTCTCT GTGACATGCA TCCCATGAGA 
GCACTGTTTC TCATCCCCAG AAACCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCAAAA 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACA 
GAGCAGCTTC TGAAACATCC TTTTATCAGG GACCAGCCGA ATGAAAGGCA GGTTAGGATC 
CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACCGAG 
TACGAGTACA GCGGGAGTGA GGAGGAGGAG GAGGAGGCGC CGGAGCAGGA GGGGGAGCCC 
AGCTCCATCG TCAACGTGCC TGGCGAGTCC ACGCTGCGCC GGGACTTCCT GAGGCTGCAG 
CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGCGGCAGC AGCTCCTGCA GGAGCAGCAG 
CTCAGGGAGC AGGAAGAATA CAAGAGGCAG CTGCTGGCGG AGAGACAGAA GCGGATCGAG 
CAGCAGAAGG AGCAGCGGCG ACGGCTGGAA GAGCAACAAA GGAGAGAGCG GGAGGCCAGG 
AGGCAGCAGG AGCGCGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
CTGGAGAGAC GGCGGCAGGA GGAAGAGGAG CGCAGGCGGG CGGAGGAGGA CAAGAGGAGA 
GTCGACAGGG AGCAGGAGTA TATCAGGCGG CAGCTGGAAG AGGAGCAGCG GCACCTGGAA 
CTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
CTGGAGGAGC AGCGGCAGAC GGAGCGGCTC CAGAGGCAGC TGCAGCAGGA GCAAGCCTAT 
CTCCTGTCTC TGCAGCATGA GCCCCGGAGG CCGCCCCCGC CGCCGGACAG GAGCAAGGCC 
GGCCTGCACG GCGCCGAGCC CAAGGCCCAG CACGAGCCCG CCGACCGGGC CCGCGAGGTG 
GAAGATAGAT TTAGGAAAAC TAACCACAGC TCCCCTGAAG CCCAGTCTAA GCAGACAGGC 
AGAGTATTGG AGCCACCAGT GCCTTCCAGA TCAGAATCTT TTTCCAATGG CAACTCCGAG 
TCTGTGCACC CTGCCCTGCA GAGACCAGCC GAGCCACAGG TTCCTGTGAG AACGACGTCC 
CGGTCCCCCG TCCTGTCCCG TCGGGATTCC CCACTGCAGG GCAGTGGGCA GCAAAACAGC 
CAAGCAGGTC AGAGAAACTC CACCAGCATC GAGCCCAGGC TGCTATGGGA GAGAGTGGAG 
AAGCTGGTGC CCAGGCCTGG CAGTGGCAGC TCCTCGGGGT CCAGCAACTC AGGATCCCAG 
CCCGGCTCCC ACCCTGGGTC TCAGAGCGGC TCCGGGGAGC GCTTCAGAGT GAGATCGTCA 
TCCAAATCTG AGGGCTCGCC ATCTCAGCGC CTTGAAAATG CAGCAAAAAA GCCTGAAGAT 
AAGAAGGAAG TTTTTAGACC TCTCAAGCCC GCTGACCTGA CGGCCCTGGC CAGAGAGCTC 
CGAGCGGCGG AAGACGTGCG GCCGCCGCAC AAGGTGACAG ACTACTCGTC GTCCAGCGAG 
GAGTCCGGGA CAACGGATGA GGAGGACGAC GACGTGGAGC AGGAAGGCCC CGAGGAGCCC 
ACCTCGGGGC CGGAGGACGC CCGAGCCGCG TCATCTCTGA ACTTGAGCAA CGGTGAGACG 
GAGTCCGTGA AAACGATGAT CGTCCACGAC GACGTGGAGA GTGAACCAGC CATGACCCCG 
TCCAAGGAGG GCACCCTGAT TGTCCGCCAG ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA 
CACAAATCTT CCTCCTCCTT TACACCTTTT ATAGACCCCA GATTACTACA GATTTCTCCA 
TCTAGTGGGA CAACAGTGAC TTCTGTGGTG GGATTTTCCT GTGATGGAAT GAGATCGGAG 
GCCATAAGGC AAGATCCTAC CCGGAAGGGC TCAGTGGTCA ATGTGAATCC CACCAACACT 
AGGCCACAGA GCGACACCCC AGAGATTCGC AAATATAAGA AGAGGTTTAA CTCCGAGATT 
CTGTGCGCTG CCTTGTGGGG AGTGAACTTG TTGGTGGGTA CAGAGAGTGG CCTGATGCTG 
CTGGACAGAA GTGGCCAAGG GAAGGTGTAT CCTCTGATCA ACAGAAGACG ATTTCAACAG 
ATGGACGTCC TGGAAGGCTT GAATGTCTTG GTGACAATAT CTGGCAAAAA GGATAAGTTA 
CGTGTCTACT ATTTGTCCTG GTTAAGAAAT AAAATACTTC ACAACGATCC GGAAGTTGAG 
AAGAAGCAGG GTTGGACGAC CGTGGGAGAT TTGGAAGGAT GCGTCCACTA TAAAGTCGTA 
AAATATGAAA GAATCAAGTT TCTGGTCATT GCTTTGAAGA GTTCTGTGGA AGTGTATGCG 
TGGGCACCCA AGCCATATCA CAAATTCATG GCCTTTAAGT CGTTTGGAGA TTTGGTGCAT 
AAGCCATTAC TGGTGGATCT CACTGTTGAG GAAGGCCAGA GGTTGAAAGT GATCTATGGA 
TCCTGTGCTG GATTCCATGC TGTTGACGTG GATTCAGGAT CAGTCTATGA CATTTATCTA 
CCAACACACA TCCAGTGCAG CATCAAACCC CACGCAATCA TCATCCTCCC CAACACGGAT 
GGAATGGAGC TTCTGGTGTG CTATGAAGAC GAAGGGGTCT ATGTGAACAC CTATGGAAGG 
ATTACCAAGG ATGTGGTTCT GCAGTGGGGA GAGATGCCAA CGTCTGTAGC GTATATTCGA 
TCCAATCAGA CGATGGGCTG GGGAGAAAAG GCCATAGAGA TCCGGTCTGT GGAGACGGGC 
CACTTGGACG GTGTGTTTAT GCACAAAAGG GCTCAAAGAC TGAAATTCTT ATGTGAACGC 
AACGACAAGG TGTTCTTTGC CTCGGTCCGG TCCGGGGGCA GCAGCCAGGT GTACTTCATG 
ACGCTGGGCA GGACCTCTCT TCTGAGCTGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019786786.1
CDS	145..3837
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEAPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRQEEEERRRAEEDKRRVDREQEYIRRQLEEEQRHLELLQQQLLQEQAMLLECRWRELEEQRQTERLQRQLQQEQAYLLSLQHEPRRPPPPPDRSKAGLHGAEPKAQHEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPADLTALARELRAAEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGPEEPTSGPEDARAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRSEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGDLVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_019931227.1
Organism	Tursiops truncatus(common bottlenose dolphin)
Definition	Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019931227.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
CCGGCTGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
AAGGGTCGAC ACGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA CGTTACTGAG 
GATGAAGAGG AGGAAATCAA GCTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGCGGGGCC GGGTCCATCA CAGACCTCGT GAAGAACACC 
AAGGGCAACA CGCTCAAGGA GGACTGGATA GCCTACATCT CCAGGGAGAT CCTGAGGGGA 
CTGGCGCATC TCCACATCCA TCACGTGATT CACCGGGACA TCAAGGGCCA GAATGTATTG 
CTGACCGAGA ACGCAGAGGT GAAGCTTGTG GATTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACCGTGGGGA GGAGAAACAC CTTCATAGGC ACCCCGTACT GGATGGCTCC CGAGGTCATC 
GCCTGTGACG AGAACCCCGA CGCCACCTAT GATTACAGGA GTGACCTGTG GTCCTGCGGC 
ATCACAGCCA TTGAGATGGC AGAAGGCGCT CCCCCTCTCT GTGACATGCA TCCCATGAGA 
GCACTGTTTC TCATCCCCAG AAACCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCAAAA 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACA 
GAGCAGCTTC TGAAACATCC TTTTATCAGG GACCAGCCGA ATGAAAGGCA GGTTAGGATC 
CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACCGAG 
TACGAGTACA GCGGGAGTGA GGAGGAGGAG GAGGAGGCGC CGGAGCAGGA GGGGGAGCCC 
AGCTCCATCG TCAACGTGCC TGGCGAGTCC ACGCTGCGCC GGGACTTCCT GAGGCTGCAG 
CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGCGGCAGC AGCTCCTGCA GGAGCAGCAG 
CTCAGGGAGC AGGAAGAATA CAAGAGGCAG CTGCTGGCGG AGAGACAGAA GCGGATCGAG 
CAGCAGAAGG AGCAGCGGCG ACGGCTGGAA GAGCAACAAA GGAGAGAGCG GGAGGCCAGG 
AGGCAGCAGG AGCGCGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
CTGGAGAGAC GGCGGCAGGA GGAAGAGGAG CGCAGGCGGG CGGAGGAGGA CAAGAGGAGA 
GTCGACAGGG AGCAGGAGTA TATCAGGCGG CAGCTGGAAG AGGAGCAGCG GCACCTGGAA 
CTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
CTGGAGGAGC AGCGGCAGAC GGAGCGGCTC CAGAGGCAGC TGCAGCAGGA GCAAGCCTAT 
CTCCTGTCTC TGCAGCATGA GCCCCGGAGG CCGCCCCCGC CGCCGGACAG GAGCAAGGCC 
GGCCTGCACG GCGCCGAGCC CAAGGCCCAG CACGAGCCCG CCGACCGGGC CCGCGAGGTG 
GAAGATAGAT TTAGGAAAAC TAACCACAGC TCCCCTGAAG CCCAGTCTAA GCAGACAGGC 
AGAGTATTGG AGCCACCAGT GCCTTCCAGA TCAGAATCTT TTTCCAATGG CAACTCCGAG 
TCTGTGCACC CTGCCCTGCA GAGACCAGCC GAGCCACAGG TTCCTGTGAG AACGACGTCC 
CGGTCCCCCG TCCTGTCCCG TCGGGATTCC CCACTGCAGG GCAGTGGGCA GCAAAACAGC 
CAAGCAGGTC AGAGAAACTC CACCAGCATC GAGCCCAGGC TGCTATGGGA GAGAGTGGAG 
AAGCTGGTGC CCAGGCCTGG CAGTGGCAGC TCCTCGGGGT CCAGCAACTC AGGATCCCAG 
CCCGGCTCCC ACCCTGGGTC TCAGAGCGGC TCCGGGGAGC GCTTCAGAGT GAGATCGTCA 
TCCAAATCTG AGGGCTCGCC ATCTCAGCGC CTTGAAAATG CAGCAAAAAA GCCTGAAGAT 
AAGAAGGAAG TTTTTAGACC TCTCAAGCCC GCTGACCTGA CGGCCCTGGC CAGAGAGCTC 
CGAGCGGCGG AAGACGTGCG GCCGCCGCAC AAGGTGACAG ACTACTCGTC GTCCAGCGAG 
GAGTCCGGGA CAACGGATGA GGAGGACGAC GACGTGGAGC AGGAAGGCCC CGAGGAGCCC 
ACCTCGGGGC CGGAGGACGC CCGAGCCGCG TCATCTCTGA ACTTGAGCAA CGGTGAGACG 
GAGTCCGTGA AAACGATGAT CGTCCACGAC GACGTGGAGA GTGAACCAGC CATGACCCCG 
TCCAAGGAGG GCACCCTGAT TGTCCGCCAG ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA 
CACAAATCTT CCTCCTCCTT TACACCTTTT ATAGACCCCA GATTACTACA GATTTCTCCA 
TCTAGTGGGA CAACAGTGAC TTCTGTGGTG GGATTTTCCT GTGATGGAAT GAGATCGGAG 
GCCATAAGGC AAGATCCTAC CCGGAAGGGC TCAGTGGTCA ATGTGAATCC CACCAACACT 
AGGCCACAGA GCGACACCCC AGAGATTCGC AAATATAAGA AGAGGTTTAA CTCCGAGATT 
CTGTGCGCTG CCTTGTGGGG AGTGAACTTG TTGGTGGGTA CAGAGAGTGG CCTGATGCTG 
CTGGACAGAA GTGGCCAAGG GAAGGTGTAT CCTCTGATCA ACAGAAGACG ATTTCAACAG 
ATGGACGTCC TGGAAGGCTT GAATGTCTTG GTGACAATAT CTGGCAAAAA GGATAAGTTA 
CGTGTCTACT ATTTGTCCTG GTTAAGAAAT AAAATACTTC ACAACGATCC GGAAGTTGAG 
AAGAAGCAGG GTTGGACGAC CGTGGGAGAT TTGGAAGGAT GCGTCCACTA TAAAGTCGTA 
AAATATGAAA GAATCAAGTT TCTGGTCATT GCTTTGAAGA GTTCTGTGGA AGTGTATGCG 
TGGGCACCCA AGCCATATCA CAAATTCATG GCCTTTAAGT CGTTTGGAGA TTTGGTGCAT 
AAGCCATTAC TGGTGGATCT CACTGTTGAG GAAGGCCAGA GGTTGAAAGT GATCTATGGA 
TCCTGTGCTG GATTCCATGC TGTTGACGTG GATTCAGGAT CAGTCTATGA CATTTATCTA 
CCAACACACA TCCAGTGCAG CATCAAACCC CACGCAATCA TCATCCTCCC CAACACGGAT 
GGAATGGAGC TTCTGGTGTG CTATGAAGAC GAAGGGGTCT ATGTGAACAC CTATGGAAGG 
ATTACCAAGG ATGTGGTTCT GCAGTGGGGA GAGATGCCAA CGTCTGTAGC GTATATTCGA 
TCCAATCAGA CGATGGGCTG GGGAGAAAAG GCCATAGAGA TCCGGTCTGT GGAGACGGGC 
CACTTGGACG GTGTGTTTAT GCACAAAAGG GCTCAAAGAC TGAAATTCTT ATGTGAACGC 
AACGACAAGG TGTTCTTTGC CTCGGTCCGG TCCGGGGGCA GCAGCCAGGT GTACTTCATG 
ACGCTGGGCA GGACCTCTCT TCTGAGCTGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_019931228.1 ) cDNA ORF clone, Tursiops truncatus(common bottlenose dolphin) -> XP_019786787.1 Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OTu39187
Clone ID Related Accession (Same CDS sequence)	XM_019931228.1
Accession Version	XM_019931228.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Tursiops truncatus(common bottlenose dolphin)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017842906.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tursiops truncatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
CCGGCTGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
AAGGGTCGAC ACGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA CGTTACTGAG 
GATGAAGAGG AGGAAATCAA GCTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGCGGGGCC GGGTCCATCA CAGACCTCGT GAAGAACACC 
AAGGGCAACA CGCTCAAGGA GGACTGGATA GCCTACATCT CCAGGGAGAT CCTGAGGGGA 
CTGGCGCATC TCCACATCCA TCACGTGATT CACCGGGACA TCAAGGGCCA GAATGTATTG 
CTGACCGAGA ACGCAGAGGT GAAGCTTGTG GATTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACCGTGGGGA GGAGAAACAC CTTCATAGGC ACCCCGTACT GGATGGCTCC CGAGGTCATC 
GCCTGTGACG AGAACCCCGA CGCCACCTAT GATTACAGGA GTGACCTGTG GTCCTGCGGC 
ATCACAGCCA TTGAGATGGC AGAAGGCGCT CCCCCTCTCT GTGACATGCA TCCCATGAGA 
GCACTGTTTC TCATCCCCAG AAACCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCAAAA 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACA 
GAGCAGCTTC TGAAACATCC TTTTATCAGG GACCAGCCGA ATGAAAGGCA GGTTAGGATC 
CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACCGAG 
TACGAGTACA GCGGGAGTGA GGAGGAGGAG GAGGAGGCGC CGGAGCAGGA GGGGGAGCCC 
AGCTCCATCG TCAACGTGCC TGGCGAGTCC ACGCTGCGCC GGGACTTCCT GAGGCTGCAG 
CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGCGGCAGC AGCTCCTGCA GGAGCAGCAG 
CTCAGGGAGC AGGAAGAATA CAAGAGGCAG CTGCTGGCGG AGAGACAGAA GCGGATCGAG 
CAGCAGAAGG AGCAGCGGCG ACGGCTGGAA GAGCAACAAA GGAGAGAGCG GGAGGCCAGG 
AGGCAGCAGG AGCGCGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
CTGGAGAGAC GGCGGCAGGA GGAAGAGGAG CGCAGGCGGG CGGAGGAGGA CAAGAGGAGA 
GTCGACAGGG AGCAGGAGTA TATCAGGCGG CAGCTGGAAG AGGAGCAGCG GCACCTGGAA 
CTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCATGAGCC CCGGAGGCCG 
CCCCCGCCGC CGGACAGGAG CAAGGCCGGC CTGCACGGCG CCGAGCCCAA GGCCCAGCAC 
GAGCCCGCCG ACCGGGCCCG CGAGGTACAG TGGTCCCACC TGGCATCTCT CAAGAACAGT 
GTTTCCCCTG TCTCGCGATC CCATTCCTTC AGTGACCCTT CTCCTCCCAA ATTTGCACAC 
CACCATCTTC GCTCTCAGGA CCCATGTCCA CCTTCCCGCA GTGAGGTGCT AAGTCAGAGT 
TCTGACTCTA AGTCGGAGGT ACCCGACCCC GCCCAAAAGG CTTGGTCTAG ATCAGACAGT 
GACGAGGTCC CTCCAAGGGT TCCTGTGAGA ACGACGTCCC GGTCCCCCGT CCTGTCCCGT 
CGGGATTCCC CACTGCAGGG CAGTGGGCAG CAAAACAGCC AAGCAGGTCA GAGAAACTCC 
ACCAGCAGCA TCGAGCCCAG GCTGCTATGG GAGAGAGTGG AGAAGCTGGT GCCCAGGCCT 
GGCAGTGGCA GCTCCTCGGG GTCCAGCAAC TCAGGATCCC AGCCCGGCTC CCACCCTGGG 
TCTCAGAGCG GCTCCGGGGA GCGCTTCAGA GTGAGATCGT CATCCAAATC TGAGGGCTCG 
CCATCTCAGC GCCTTGAAAA TGCAGCAAAA AAGCCTGAAG ATAAGAAGGA AGTTTTTAGA 
CCTCTCAAGC CCGCTGGCGA AGTGGACCTG ACGGCCCTGG CCAGAGAGCT CCGAGCGGCG 
GAAGACGTGC GGCCGCCGCA CAAGGTGACA GACTACTCGT CGTCCAGCGA GGAGTCCGGG 
ACAACGGATG AGGAGGACGA CGACGTGGAG CAGGAAGGCC CCGAGGAGCC CACCTCGGGG 
CCGGAGGACG CCCGAGCCGC GTCATCTCTG AACTTGAGCA ACGGTGAGAC GGAGTCCGTG 
AAAACGATGA TCGTCCACGA CGACGTGGAG AGTGAACCAG CCATGACCCC GTCCAAGGAG 
GGCACCCTGA TTGTCCGCCA GACTCAGTCC GCTAGTAGCA CACTCCAGAA ACACAAATCT 
TCCTCCTCCT TTACACCTTT TATAGACCCC AGATTACTAC AGATTTCTCC ATCTAGTGGG 
ACAACAGTGA CTTCTGTGGT GGGATTTTCC TGTGATGGAA TGAGATCGGA GGCCATAAGG 
CAAGATCCTA CCCGGAAGGG CTCAGTGGTC AATGTGAATC CCACCAACAC TAGGCCACAG 
AGCGACACCC CAGAGATTCG CAAATATAAG AAGAGGTTTA ACTCCGAGAT TCTGTGCGCT 
GCCTTGTGGG GAGTGAACTT GTTGGTGGGT ACAGAGAGTG GCCTGATGCT GCTGGACAGA 
AGTGGCCAAG GGAAGGTGTA TCCTCTGATC AACAGAAGAC GATTTCAACA GATGGACGTC 
CTGGAAGGCT TGAATGTCTT GGTGACAATA TCTGGCAAAA AGGATAAGTT ACGTGTCTAC 
TATTTGTCCT GGTTAAGAAA TAAAATACTT CACAACGATC CGGAAGTTGA GAAGAAGCAG 
GGTTGGACGA CCGTGGGAGA TTTGGAAGGA TGCGTCCACT ATAAAGTCGT AAAATATGAA 
AGAATCAAGT TTCTGGTCAT TGCTTTGAAG AGTTCTGTGG AAGTGTATGC GTGGGCACCC 
AAGCCATATC ACAAATTCAT GGCCTTTAAG TCGTTTGGAG ATTTGGTGCA TAAGCCATTA 
CTGGTGGATC TCACTGTTGA GGAAGGCCAG AGGTTGAAAG TGATCTATGG ATCCTGTGCT 
GGATTCCATG CTGTTGACGT GGATTCAGGA TCAGTCTATG ACATTTATCT ACCAACACAC 
ATCCAGTGCA GCATCAAACC CCACGCAATC ATCATCCTCC CCAACACGGA TGGAATGGAG 
CTTCTGGTGT GCTATGAAGA CGAAGGGGTC TATGTGAACA CCTATGGAAG GATTACCAAG 
GATGTGGTTC TGCAGTGGGG AGAGATGCCA ACGTCTGTAG CGTATATTCG ATCCAATCAG 
ACGATGGGCT GGGGAGAAAA GGCCATAGAG ATCCGGTCTG TGGAGACGGG CCACTTGGAC 
GGTGTGTTTA TGCACAAAAG GGCTCAAAGA CTGAAATTCT TATGTGAACG CAACGACAAG 
GTGTTCTTTG CCTCGGTCCG GTCCGGGGGC AGCAGCCAGG TGTACTTCAT GACGCTGGGC 
AGGACCTCTC TTCTGAGCTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019786787.1
CDS	147..3830
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEAPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRQEEEERRRAEEDKRRVDREQEYIRRQLEEEQRHLELLQQQLLQEQAMLLHEPRRPPPPPDRSKAGLHGAEPKAQHEPADRAREVQWSHLASLKNSVSPVSRSHSFSDPSPPKFAHHHLRSQDPCPPSRSEVLSQSSDSKSEVPDPAQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPAGEVDLTALARELRAAEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGPEEPTSGPEDARAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRSEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGDLVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_019931228.1
Organism	Tursiops truncatus(common bottlenose dolphin)
Definition	Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019931228.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
CCGGCTGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
AAGGGTCGAC ACGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA CGTTACTGAG 
GATGAAGAGG AGGAAATCAA GCTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGCGGGGCC GGGTCCATCA CAGACCTCGT GAAGAACACC 
AAGGGCAACA CGCTCAAGGA GGACTGGATA GCCTACATCT CCAGGGAGAT CCTGAGGGGA 
CTGGCGCATC TCCACATCCA TCACGTGATT CACCGGGACA TCAAGGGCCA GAATGTATTG 
CTGACCGAGA ACGCAGAGGT GAAGCTTGTG GATTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACCGTGGGGA GGAGAAACAC CTTCATAGGC ACCCCGTACT GGATGGCTCC CGAGGTCATC 
GCCTGTGACG AGAACCCCGA CGCCACCTAT GATTACAGGA GTGACCTGTG GTCCTGCGGC 
ATCACAGCCA TTGAGATGGC AGAAGGCGCT CCCCCTCTCT GTGACATGCA TCCCATGAGA 
GCACTGTTTC TCATCCCCAG AAACCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCAAAA 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACA 
GAGCAGCTTC TGAAACATCC TTTTATCAGG GACCAGCCGA ATGAAAGGCA GGTTAGGATC 
CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACCGAG 
TACGAGTACA GCGGGAGTGA GGAGGAGGAG GAGGAGGCGC CGGAGCAGGA GGGGGAGCCC 
AGCTCCATCG TCAACGTGCC TGGCGAGTCC ACGCTGCGCC GGGACTTCCT GAGGCTGCAG 
CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGCGGCAGC AGCTCCTGCA GGAGCAGCAG 
CTCAGGGAGC AGGAAGAATA CAAGAGGCAG CTGCTGGCGG AGAGACAGAA GCGGATCGAG 
CAGCAGAAGG AGCAGCGGCG ACGGCTGGAA GAGCAACAAA GGAGAGAGCG GGAGGCCAGG 
AGGCAGCAGG AGCGCGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
CTGGAGAGAC GGCGGCAGGA GGAAGAGGAG CGCAGGCGGG CGGAGGAGGA CAAGAGGAGA 
GTCGACAGGG AGCAGGAGTA TATCAGGCGG CAGCTGGAAG AGGAGCAGCG GCACCTGGAA 
CTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCATGAGCC CCGGAGGCCG 
CCCCCGCCGC CGGACAGGAG CAAGGCCGGC CTGCACGGCG CCGAGCCCAA GGCCCAGCAC 
GAGCCCGCCG ACCGGGCCCG CGAGGTACAG TGGTCCCACC TGGCATCTCT CAAGAACAGT 
GTTTCCCCTG TCTCGCGATC CCATTCCTTC AGTGACCCTT CTCCTCCCAA ATTTGCACAC 
CACCATCTTC GCTCTCAGGA CCCATGTCCA CCTTCCCGCA GTGAGGTGCT AAGTCAGAGT 
TCTGACTCTA AGTCGGAGGT ACCCGACCCC GCCCAAAAGG CTTGGTCTAG ATCAGACAGT 
GACGAGGTCC CTCCAAGGGT TCCTGTGAGA ACGACGTCCC GGTCCCCCGT CCTGTCCCGT 
CGGGATTCCC CACTGCAGGG CAGTGGGCAG CAAAACAGCC AAGCAGGTCA GAGAAACTCC 
ACCAGCAGCA TCGAGCCCAG GCTGCTATGG GAGAGAGTGG AGAAGCTGGT GCCCAGGCCT 
GGCAGTGGCA GCTCCTCGGG GTCCAGCAAC TCAGGATCCC AGCCCGGCTC CCACCCTGGG 
TCTCAGAGCG GCTCCGGGGA GCGCTTCAGA GTGAGATCGT CATCCAAATC TGAGGGCTCG 
CCATCTCAGC GCCTTGAAAA TGCAGCAAAA AAGCCTGAAG ATAAGAAGGA AGTTTTTAGA 
CCTCTCAAGC CCGCTGGCGA AGTGGACCTG ACGGCCCTGG CCAGAGAGCT CCGAGCGGCG 
GAAGACGTGC GGCCGCCGCA CAAGGTGACA GACTACTCGT CGTCCAGCGA GGAGTCCGGG 
ACAACGGATG AGGAGGACGA CGACGTGGAG CAGGAAGGCC CCGAGGAGCC CACCTCGGGG 
CCGGAGGACG CCCGAGCCGC GTCATCTCTG AACTTGAGCA ACGGTGAGAC GGAGTCCGTG 
AAAACGATGA TCGTCCACGA CGACGTGGAG AGTGAACCAG CCATGACCCC GTCCAAGGAG 
GGCACCCTGA TTGTCCGCCA GACTCAGTCC GCTAGTAGCA CACTCCAGAA ACACAAATCT 
TCCTCCTCCT TTACACCTTT TATAGACCCC AGATTACTAC AGATTTCTCC ATCTAGTGGG 
ACAACAGTGA CTTCTGTGGT GGGATTTTCC TGTGATGGAA TGAGATCGGA GGCCATAAGG 
CAAGATCCTA CCCGGAAGGG CTCAGTGGTC AATGTGAATC CCACCAACAC TAGGCCACAG 
AGCGACACCC CAGAGATTCG CAAATATAAG AAGAGGTTTA ACTCCGAGAT TCTGTGCGCT 
GCCTTGTGGG GAGTGAACTT GTTGGTGGGT ACAGAGAGTG GCCTGATGCT GCTGGACAGA 
AGTGGCCAAG GGAAGGTGTA TCCTCTGATC AACAGAAGAC GATTTCAACA GATGGACGTC 
CTGGAAGGCT TGAATGTCTT GGTGACAATA TCTGGCAAAA AGGATAAGTT ACGTGTCTAC 
TATTTGTCCT GGTTAAGAAA TAAAATACTT CACAACGATC CGGAAGTTGA GAAGAAGCAG 
GGTTGGACGA CCGTGGGAGA TTTGGAAGGA TGCGTCCACT ATAAAGTCGT AAAATATGAA 
AGAATCAAGT TTCTGGTCAT TGCTTTGAAG AGTTCTGTGG AAGTGTATGC GTGGGCACCC 
AAGCCATATC ACAAATTCAT GGCCTTTAAG TCGTTTGGAG ATTTGGTGCA TAAGCCATTA 
CTGGTGGATC TCACTGTTGA GGAAGGCCAG AGGTTGAAAG TGATCTATGG ATCCTGTGCT 
GGATTCCATG CTGTTGACGT GGATTCAGGA TCAGTCTATG ACATTTATCT ACCAACACAC 
ATCCAGTGCA GCATCAAACC CCACGCAATC ATCATCCTCC CCAACACGGA TGGAATGGAG 
CTTCTGGTGT GCTATGAAGA CGAAGGGGTC TATGTGAACA CCTATGGAAG GATTACCAAG 
GATGTGGTTC TGCAGTGGGG AGAGATGCCA ACGTCTGTAG CGTATATTCG ATCCAATCAG 
ACGATGGGCT GGGGAGAAAA GGCCATAGAG ATCCGGTCTG TGGAGACGGG CCACTTGGAC 
GGTGTGTTTA TGCACAAAAG GGCTCAAAGA CTGAAATTCT TATGTGAACG CAACGACAAG 
GTGTTCTTTG CCTCGGTCCG GTCCGGGGGC AGCAGCCAGG TGTACTTCAT GACGCTGGGC 
AGGACCTCTC TTCTGAGCTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_019931229.1 ) cDNA ORF clone, Tursiops truncatus(common bottlenose dolphin) -> XP_019786788.1 Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OTu39188
Clone ID Related Accession (Same CDS sequence)	XM_019931229.1
Accession Version	XM_019931229.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Tursiops truncatus(common bottlenose dolphin)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017842906.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tursiops truncatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
CCGGCTGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
AAGGGTCGAC ACGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA CGTTACTGAG 
GATGAAGAGG AGGAAATCAA GCTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGCGGGGCC GGGTCCATCA CAGACCTCGT GAAGAACACC 
AAGGGCAACA CGCTCAAGGA GGACTGGATA GCCTACATCT CCAGGGAGAT CCTGAGGGGA 
CTGGCGCATC TCCACATCCA TCACGTGATT CACCGGGACA TCAAGGGCCA GAATGTATTG 
CTGACCGAGA ACGCAGAGGT GAAGCTTGTG GATTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACCGTGGGGA GGAGAAACAC CTTCATAGGC ACCCCGTACT GGATGGCTCC CGAGGTCATC 
GCCTGTGACG AGAACCCCGA CGCCACCTAT GATTACAGGA GTGACCTGTG GTCCTGCGGC 
ATCACAGCCA TTGAGATGGC AGAAGGCGCT CCCCCTCTCT GTGACATGCA TCCCATGAGA 
GCACTGTTTC TCATCCCCAG AAACCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCAAAA 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACA 
GAGCAGCTTC TGAAACATCC TTTTATCAGG GACCAGCCGA ATGAAAGGCA GGTTAGGATC 
CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACCGAG 
TACGAGTACA GCGGGAGTGA GGAGGAGGAG GAGGAGGCGC CGGAGCAGGA GGGGGAGCCC 
AGCTCCATCG TCAACGTGCC TGGCGAGTCC ACGCTGCGCC GGGACTTCCT GAGGCTGCAG 
CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGCGGCAGC AGCTCCTGCA GGAGCAGCAG 
CTCAGGGAGC AGGAAGAATA CAAGAGGCAG CTGCTGGCGG AGAGACAGAA GCGGATCGAG 
CAGCAGAAGG AGCAGCGGCG ACGGCTGGAA GAGCAACAAA GGAGAGAGCG GGAGGCCAGG 
AGGCAGCAGG AGCGCGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
CTGGAGAGAC GGCGGCAGGA GGAAGAGGAG CGCAGGCGGG CGGAGGAGGA CAAGAGGAGA 
GTCGACAGGG AGCAGGAGTA TATCAGGCGG CAGCTGGAAG AGGAGCAGCG GCACCTGGAA 
CTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCATGAGCC CCGGAGGCCG 
CCCCCGCCGC CGGACAGGAG CAAGGCCGGC CTGCACGGCG CCGAGCCCAA GGCCCAGCAC 
GAGCCCGCCG ACCGGGCCCG CGAGGTTCCT GTGAGAACGA CGTCCCGGTC CCCCGTCCTG 
TCCCGTCGGG ATTCCCCACT GCAGGGCAGT GGGCAGCAAA ACAGCCAAGC AGGTCAGAGA 
AACTCCACCA GCAGCATCGA GCCCAGGCTG CTATGGGAGA GAGTGGAGAA GCTGGTGCCC 
AGGCCTGGCA GTGGCAGCTC CTCGGGGTCC AGCAACTCAG GATCCCAGCC CGGCTCCCAC 
CCTGGGTCTC AGAGCGGCTC CGGGGAGCGC TTCAGAGTGA GATCGTCATC CAAATCTGAG 
GGCTCGCCAT CTCAGCGCCT TGAAAATGCA GCAAAAAAGC CTGAAGATAA GAAGGAAGTT 
TTTAGACCTC TCAAGCCCGC TGGCGAAGTG GACCTGACGG CCCTGGCCAG AGAGCTCCGA 
GCGGCGGAAG ACGTGCGGCC GCCGCACAAG GTGACAGACT ACTCGTCGTC CAGCGAGGAG 
TCCGGGACAA CGGATGAGGA GGACGACGAC GTGGAGCAGG AAGGCCCCGA GGAGCCCACC 
TCGGGGCCGG AGGACGCCCG AGCCGCGTCA TCTCTGAACT TGAGCAACGG TGAGACGGAG 
TCCGTGAAAA CGATGATCGT CCACGACGAC GTGGAGAGTG AACCAGCCAT GACCCCGTCC 
AAGGAGGGCA CCCTGATTGT CCGCCAGAGT GCAGTTGACC AAAAGCGCGC CAGCCATCAT 
GAGAGCAATG GCTTTGCCGG TCGCGTTCAC CTCTTGCCAG ATCTCTTACA GCAAAGCCAT 
TCCTCCTCCA CTTCCTCCAC CTCCTCCTCC CCATCCTCCA GCCAGCCGAC ACCCACCATG 
TCCCCACAGA CACCCCAGGA CAAGCTCACT CCTAACGAGA CTCAGTCCGC TAGTAGCACA 
CTCCAGAAAC ACAAATCTTC CTCCTCCTTT ACACCTTTTA TAGACCCCAG ATTACTACAG 
ATTTCTCCAT CTAGTGGGAC AACAGTGACT TCTGTGGTGG GATTTTCCTG TGATGGAATG 
AGATCGGAGG CCATAAGGCA AGATCCTACC CGGAAGGGCT CAGTGGTCAA TGTGAATCCC 
ACCAACACTA GGCCACAGAG CGACACCCCA GAGATTCGCA AATATAAGAA GAGGTTTAAC 
TCCGAGATTC TGTGCGCTGC CTTGTGGGGA GTGAACTTGT TGGTGGGTAC AGAGAGTGGC 
CTGATGCTGC TGGACAGAAG TGGCCAAGGG AAGGTGTATC CTCTGATCAA CAGAAGACGA 
TTTCAACAGA TGGACGTCCT GGAAGGCTTG AATGTCTTGG TGACAATATC TGGCAAAAAG 
GATAAGTTAC GTGTCTACTA TTTGTCCTGG TTAAGAAATA AAATACTTCA CAACGATCCG 
GAAGTTGAGA AGAAGCAGGG TTGGACGACC GTGGGAGATT TGGAAGGATG CGTCCACTAT 
AAAGTCGTAA AATATGAAAG AATCAAGTTT CTGGTCATTG CTTTGAAGAG TTCTGTGGAA 
GTGTATGCGT GGGCACCCAA GCCATATCAC AAATTCATGG CCTTTAAGTC GTTTGGAGAT 
TTGGTGCATA AGCCATTACT GGTGGATCTC ACTGTTGAGG AAGGCCAGAG GTTGAAAGTG 
ATCTATGGAT CCTGTGCTGG ATTCCATGCT GTTGACGTGG ATTCAGGATC AGTCTATGAC 
ATTTATCTAC CAACACACAT CCAGTGCAGC ATCAAACCCC ACGCAATCAT CATCCTCCCC 
AACACGGATG GAATGGAGCT TCTGGTGTGC TATGAAGACG AAGGGGTCTA TGTGAACACC 
TATGGAAGGA TTACCAAGGA TGTGGTTCTG CAGTGGGGAG AGATGCCAAC GTCTGTAGCG 
TATATTCGAT CCAATCAGAC GATGGGCTGG GGAGAAAAGG CCATAGAGAT CCGGTCTGTG 
GAGACGGGCC ACTTGGACGG TGTGTTTATG CACAAAAGGG CTCAAAGACT GAAATTCTTA 
TGTGAACGCA ACGACAAGGT GTTCTTTGCC TCGGTCCGGT CCGGGGGCAG CAGCCAGGTG 
TACTTCATGA CGCTGGGCAG GACCTCTCTT CTGAGCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019786788.1
CDS	146..3787
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEAPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRQEEEERRRAEEDKRRVDREQEYIRRQLEEEQRHLELLQQQLLQEQAMLLHEPRRPPPPPDRSKAGLHGAEPKAQHEPADRAREVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPAGEVDLTALARELRAAEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGPEEPTSGPEDARAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSAVDQKRASHHESNGFAGRVHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTPNETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRSEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGDLVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_019931229.1
Organism	Tursiops truncatus(common bottlenose dolphin)
Definition	Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019931229.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
CCGGCTGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
AAGGGTCGAC ACGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA CGTTACTGAG 
GATGAAGAGG AGGAAATCAA GCTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGCGGGGCC GGGTCCATCA CAGACCTCGT GAAGAACACC 
AAGGGCAACA CGCTCAAGGA GGACTGGATA GCCTACATCT CCAGGGAGAT CCTGAGGGGA 
CTGGCGCATC TCCACATCCA TCACGTGATT CACCGGGACA TCAAGGGCCA GAATGTATTG 
CTGACCGAGA ACGCAGAGGT GAAGCTTGTG GATTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACCGTGGGGA GGAGAAACAC CTTCATAGGC ACCCCGTACT GGATGGCTCC CGAGGTCATC 
GCCTGTGACG AGAACCCCGA CGCCACCTAT GATTACAGGA GTGACCTGTG GTCCTGCGGC 
ATCACAGCCA TTGAGATGGC AGAAGGCGCT CCCCCTCTCT GTGACATGCA TCCCATGAGA 
GCACTGTTTC TCATCCCCAG AAACCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCAAAA 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACA 
GAGCAGCTTC TGAAACATCC TTTTATCAGG GACCAGCCGA ATGAAAGGCA GGTTAGGATC 
CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACCGAG 
TACGAGTACA GCGGGAGTGA GGAGGAGGAG GAGGAGGCGC CGGAGCAGGA GGGGGAGCCC 
AGCTCCATCG TCAACGTGCC TGGCGAGTCC ACGCTGCGCC GGGACTTCCT GAGGCTGCAG 
CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGCGGCAGC AGCTCCTGCA GGAGCAGCAG 
CTCAGGGAGC AGGAAGAATA CAAGAGGCAG CTGCTGGCGG AGAGACAGAA GCGGATCGAG 
CAGCAGAAGG AGCAGCGGCG ACGGCTGGAA GAGCAACAAA GGAGAGAGCG GGAGGCCAGG 
AGGCAGCAGG AGCGCGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
CTGGAGAGAC GGCGGCAGGA GGAAGAGGAG CGCAGGCGGG CGGAGGAGGA CAAGAGGAGA 
GTCGACAGGG AGCAGGAGTA TATCAGGCGG CAGCTGGAAG AGGAGCAGCG GCACCTGGAA 
CTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCATGAGCC CCGGAGGCCG 
CCCCCGCCGC CGGACAGGAG CAAGGCCGGC CTGCACGGCG CCGAGCCCAA GGCCCAGCAC 
GAGCCCGCCG ACCGGGCCCG CGAGGTTCCT GTGAGAACGA CGTCCCGGTC CCCCGTCCTG 
TCCCGTCGGG ATTCCCCACT GCAGGGCAGT GGGCAGCAAA ACAGCCAAGC AGGTCAGAGA 
AACTCCACCA GCAGCATCGA GCCCAGGCTG CTATGGGAGA GAGTGGAGAA GCTGGTGCCC 
AGGCCTGGCA GTGGCAGCTC CTCGGGGTCC AGCAACTCAG GATCCCAGCC CGGCTCCCAC 
CCTGGGTCTC AGAGCGGCTC CGGGGAGCGC TTCAGAGTGA GATCGTCATC CAAATCTGAG 
GGCTCGCCAT CTCAGCGCCT TGAAAATGCA GCAAAAAAGC CTGAAGATAA GAAGGAAGTT 
TTTAGACCTC TCAAGCCCGC TGGCGAAGTG GACCTGACGG CCCTGGCCAG AGAGCTCCGA 
GCGGCGGAAG ACGTGCGGCC GCCGCACAAG GTGACAGACT ACTCGTCGTC CAGCGAGGAG 
TCCGGGACAA CGGATGAGGA GGACGACGAC GTGGAGCAGG AAGGCCCCGA GGAGCCCACC 
TCGGGGCCGG AGGACGCCCG AGCCGCGTCA TCTCTGAACT TGAGCAACGG TGAGACGGAG 
TCCGTGAAAA CGATGATCGT CCACGACGAC GTGGAGAGTG AACCAGCCAT GACCCCGTCC 
AAGGAGGGCA CCCTGATTGT CCGCCAGAGT GCAGTTGACC AAAAGCGCGC CAGCCATCAT 
GAGAGCAATG GCTTTGCCGG TCGCGTTCAC CTCTTGCCAG ATCTCTTACA GCAAAGCCAT 
TCCTCCTCCA CTTCCTCCAC CTCCTCCTCC CCATCCTCCA GCCAGCCGAC ACCCACCATG 
TCCCCACAGA CACCCCAGGA CAAGCTCACT CCTAACGAGA CTCAGTCCGC TAGTAGCACA 
CTCCAGAAAC ACAAATCTTC CTCCTCCTTT ACACCTTTTA TAGACCCCAG ATTACTACAG 
ATTTCTCCAT CTAGTGGGAC AACAGTGACT TCTGTGGTGG GATTTTCCTG TGATGGAATG 
AGATCGGAGG CCATAAGGCA AGATCCTACC CGGAAGGGCT CAGTGGTCAA TGTGAATCCC 
ACCAACACTA GGCCACAGAG CGACACCCCA GAGATTCGCA AATATAAGAA GAGGTTTAAC 
TCCGAGATTC TGTGCGCTGC CTTGTGGGGA GTGAACTTGT TGGTGGGTAC AGAGAGTGGC 
CTGATGCTGC TGGACAGAAG TGGCCAAGGG AAGGTGTATC CTCTGATCAA CAGAAGACGA 
TTTCAACAGA TGGACGTCCT GGAAGGCTTG AATGTCTTGG TGACAATATC TGGCAAAAAG 
GATAAGTTAC GTGTCTACTA TTTGTCCTGG TTAAGAAATA AAATACTTCA CAACGATCCG 
GAAGTTGAGA AGAAGCAGGG TTGGACGACC GTGGGAGATT TGGAAGGATG CGTCCACTAT 
AAAGTCGTAA AATATGAAAG AATCAAGTTT CTGGTCATTG CTTTGAAGAG TTCTGTGGAA 
GTGTATGCGT GGGCACCCAA GCCATATCAC AAATTCATGG CCTTTAAGTC GTTTGGAGAT 
TTGGTGCATA AGCCATTACT GGTGGATCTC ACTGTTGAGG AAGGCCAGAG GTTGAAAGTG 
ATCTATGGAT CCTGTGCTGG ATTCCATGCT GTTGACGTGG ATTCAGGATC AGTCTATGAC 
ATTTATCTAC CAACACACAT CCAGTGCAGC ATCAAACCCC ACGCAATCAT CATCCTCCCC 
AACACGGATG GAATGGAGCT TCTGGTGTGC TATGAAGACG AAGGGGTCTA TGTGAACACC 
TATGGAAGGA TTACCAAGGA TGTGGTTCTG CAGTGGGGAG AGATGCCAAC GTCTGTAGCG 
TATATTCGAT CCAATCAGAC GATGGGCTGG GGAGAAAAGG CCATAGAGAT CCGGTCTGTG 
GAGACGGGCC ACTTGGACGG TGTGTTTATG CACAAAAGGG CTCAAAGACT GAAATTCTTA 
TGTGAACGCA ACGACAAGGT GTTCTTTGCC TCGGTCCGGT CCGGGGGCAG CAGCCAGGTG 
TACTTCATGA CGCTGGGCAG GACCTCTCTT CTGAGCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_019931230.1 ) cDNA ORF clone, Tursiops truncatus(common bottlenose dolphin) -> XP_019786789.1 Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OTu39189
Clone ID Related Accession (Same CDS sequence)	XM_019931230.1
Accession Version	XM_019931230.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Tursiops truncatus(common bottlenose dolphin)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017842906.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tursiops truncatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
CCGGCTGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
AAGGGTCGAC ACGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA CGTTACTGAG 
GATGAAGAGG AGGAAATCAA GCTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGCGGGGCC GGGTCCATCA CAGACCTCGT GAAGAACACC 
AAGGGCAACA CGCTCAAGGA GGACTGGATA GCCTACATCT CCAGGGAGAT CCTGAGGGGA 
CTGGCGCATC TCCACATCCA TCACGTGATT CACCGGGACA TCAAGGGCCA GAATGTATTG 
CTGACCGAGA ACGCAGAGGT GAAGCTTGTG GATTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACCGTGGGGA GGAGAAACAC CTTCATAGGC ACCCCGTACT GGATGGCTCC CGAGGTCATC 
GCCTGTGACG AGAACCCCGA CGCCACCTAT GATTACAGGA GTGACCTGTG GTCCTGCGGC 
ATCACAGCCA TTGAGATGGC AGAAGGCGCT CCCCCTCTCT GTGACATGCA TCCCATGAGA 
GCACTGTTTC TCATCCCCAG AAACCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCAAAA 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACA 
GAGCAGCTTC TGAAACATCC TTTTATCAGG GACCAGCCGA ATGAAAGGCA GGTTAGGATC 
CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACCGAG 
TACGAGTACA GCGGGAGTGA GGAGGAGGAG GAGGAGGCGC CGGAGCAGGA GGGGGAGCCC 
AGCTCCATCG TCAACGTGCC TGGCGAGTCC ACGCTGCGCC GGGACTTCCT GAGGCTGCAG 
CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGCGGCAGC AGCTCCTGCA GGAGCAGCAG 
CTCAGGGAGC AGGAAGAATA CAAGAGGCAG CTGCTGGCGG AGAGACAGAA GCGGATCGAG 
CAGCAGAAGG AGCAGCGGCG ACGGCTGGAA GAGCAACAAA GGAGAGAGCG GGAGGCCAGG 
AGGCAGCAGG AGCGCGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
CTGGAGAGAC GGCGGCAGGA GGAAGAGGAG CGCAGGCGGG CGGAGGAGGA CAAGAGGAGA 
GTCGACAGGG AGCAGGAGTA TATCAGGCGG CAGCTGGAAG AGGAGCAGCG GCACCTGGAA 
CTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCATGAGCC CCGGAGGCCG 
CCCCCGCCGC CGGACAGGAG CAAGGCCGGC CTGCACGGCG CCGAGCCCAA GGCCCAGCAC 
GAGCCCGCCG ACCGGGCCCG CGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCAGATCA 
GAATCTTTTT CCAATGGCAA CTCCGAGTCT GTGCACCCTG CCCTGCAGAG ACCAGCCGAG 
CCACAGGTTC CTGTGAGAAC GACGTCCCGG TCCCCCGTCC TGTCCCGTCG GGATTCCCCA 
CTGCAGGGCA GTGGGCAGCA AAACAGCCAA GCAGGTCAGA GAAACTCCAC CAGCAGCATC 
GAGCCCAGGC TGCTATGGGA GAGAGTGGAG AAGCTGGTGC CCAGGCCTGG CAGTGGCAGC 
TCCTCGGGGT CCAGCAACTC AGGATCCCAG CCCGGCTCCC ACCCTGGGTC TCAGAGCGGC 
TCCGGGGAGC GCTTCAGAGT GAGATCGTCA TCCAAATCTG AGGGCTCGCC ATCTCAGCGC 
CTTGAAAATG CAGCAAAAAA GCCTGAAGAT AAGAAGGAAG TTTTTAGACC TCTCAAGCCC 
GCTGGCGAAG TGGACCTGAC GGCCCTGGCC AGAGAGCTCC GAGCGGCGGA AGACGTGCGG 
CCGCCGCACA AGGTGACAGA CTACTCGTCG TCCAGCGAGG AGTCCGGGAC AACGGATGAG 
GAGGACGACG ACGTGGAGCA GGAAGGCCCC GAGGAGCCCA CCTCGGGGCC GGAGGACGCC 
CGAGCCGCGT CATCTCTGAA CTTGAGCAAC GGTGAGACGG AGTCCGTGAA AACGATGATC 
GTCCACGACG ACGTGGAGAG TGAACCAGCC ATGACCCCGT CCAAGGAGGG CACCCTGATT 
GTCCGCCAGA CTCAGTCCGC TAGTAGCACA CTCCAGAAAC ACAAATCTTC CTCCTCCTTT 
ACACCTTTTA TAGACCCCAG ATTACTACAG ATTTCTCCAT CTAGTGGGAC AACAGTGACT 
TCTGTGGTGG GATTTTCCTG TGATGGAATG AGATCGGAGG CCATAAGGCA AGATCCTACC 
CGGAAGGGCT CAGTGGTCAA TGTGAATCCC ACCAACACTA GGCCACAGAG CGACACCCCA 
GAGATTCGCA AATATAAGAA GAGGTTTAAC TCCGAGATTC TGTGCGCTGC CTTGTGGGGA 
GTGAACTTGT TGGTGGGTAC AGAGAGTGGC CTGATGCTGC TGGACAGAAG TGGCCAAGGG 
AAGGTGTATC CTCTGATCAA CAGAAGACGA TTTCAACAGA TGGACGTCCT GGAAGGCTTG 
AATGTCTTGG TGACAATATC TGGCAAAAAG GATAAGTTAC GTGTCTACTA TTTGTCCTGG 
TTAAGAAATA AAATACTTCA CAACGATCCG GAAGTTGAGA AGAAGCAGGG TTGGACGACC 
GTGGGAGATT TGGAAGGATG CGTCCACTAT AAAGTCGTAA AATATGAAAG AATCAAGTTT 
CTGGTCATTG CTTTGAAGAG TTCTGTGGAA GTGTATGCGT GGGCACCCAA GCCATATCAC 
AAATTCATGG CCTTTAAGTC GTTTGGAGAT TTGGTGCATA AGCCATTACT GGTGGATCTC 
ACTGTTGAGG AAGGCCAGAG GTTGAAAGTG ATCTATGGAT CCTGTGCTGG ATTCCATGCT 
GTTGACGTGG ATTCAGGATC AGTCTATGAC ATTTATCTAC CAACACACAT CCAGTGCAGC 
ATCAAACCCC ACGCAATCAT CATCCTCCCC AACACGGATG GAATGGAGCT TCTGGTGTGC 
TATGAAGACG AAGGGGTCTA TGTGAACACC TATGGAAGGA TTACCAAGGA TGTGGTTCTG 
CAGTGGGGAG AGATGCCAAC GTCTGTAGCG TATATTCGAT CCAATCAGAC GATGGGCTGG 
GGAGAAAAGG CCATAGAGAT CCGGTCTGTG GAGACGGGCC ACTTGGACGG TGTGTTTATG 
CACAAAAGGG CTCAAAGACT GAAATTCTTA TGTGAACGCA ACGACAAGGT GTTCTTTGCC 
TCGGTCCGGT CCGGGGGCAG CAGCCAGGTG TACTTCATGA CGCTGGGCAG GACCTCTCTT 
CTGAGCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019786789.1
CDS	146..3757
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEAPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRQEEEERRRAEEDKRRVDREQEYIRRQLEEEQRHLELLQQQLLQEQAMLLHEPRRPPPPPDRSKAGLHGAEPKAQHEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPAGEVDLTALARELRAAEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGPEEPTSGPEDARAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRSEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGDLVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_019931230.1
Organism	Tursiops truncatus(common bottlenose dolphin)
Definition	Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019931230.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
CCGGCTGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
AAGGGTCGAC ACGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA CGTTACTGAG 
GATGAAGAGG AGGAAATCAA GCTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGCGGGGCC GGGTCCATCA CAGACCTCGT GAAGAACACC 
AAGGGCAACA CGCTCAAGGA GGACTGGATA GCCTACATCT CCAGGGAGAT CCTGAGGGGA 
CTGGCGCATC TCCACATCCA TCACGTGATT CACCGGGACA TCAAGGGCCA GAATGTATTG 
CTGACCGAGA ACGCAGAGGT GAAGCTTGTG GATTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACCGTGGGGA GGAGAAACAC CTTCATAGGC ACCCCGTACT GGATGGCTCC CGAGGTCATC 
GCCTGTGACG AGAACCCCGA CGCCACCTAT GATTACAGGA GTGACCTGTG GTCCTGCGGC 
ATCACAGCCA TTGAGATGGC AGAAGGCGCT CCCCCTCTCT GTGACATGCA TCCCATGAGA 
GCACTGTTTC TCATCCCCAG AAACCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCAAAA 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACA 
GAGCAGCTTC TGAAACATCC TTTTATCAGG GACCAGCCGA ATGAAAGGCA GGTTAGGATC 
CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACCGAG 
TACGAGTACA GCGGGAGTGA GGAGGAGGAG GAGGAGGCGC CGGAGCAGGA GGGGGAGCCC 
AGCTCCATCG TCAACGTGCC TGGCGAGTCC ACGCTGCGCC GGGACTTCCT GAGGCTGCAG 
CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGCGGCAGC AGCTCCTGCA GGAGCAGCAG 
CTCAGGGAGC AGGAAGAATA CAAGAGGCAG CTGCTGGCGG AGAGACAGAA GCGGATCGAG 
CAGCAGAAGG AGCAGCGGCG ACGGCTGGAA GAGCAACAAA GGAGAGAGCG GGAGGCCAGG 
AGGCAGCAGG AGCGCGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
CTGGAGAGAC GGCGGCAGGA GGAAGAGGAG CGCAGGCGGG CGGAGGAGGA CAAGAGGAGA 
GTCGACAGGG AGCAGGAGTA TATCAGGCGG CAGCTGGAAG AGGAGCAGCG GCACCTGGAA 
CTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCATGAGCC CCGGAGGCCG 
CCCCCGCCGC CGGACAGGAG CAAGGCCGGC CTGCACGGCG CCGAGCCCAA GGCCCAGCAC 
GAGCCCGCCG ACCGGGCCCG CGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCAGATCA 
GAATCTTTTT CCAATGGCAA CTCCGAGTCT GTGCACCCTG CCCTGCAGAG ACCAGCCGAG 
CCACAGGTTC CTGTGAGAAC GACGTCCCGG TCCCCCGTCC TGTCCCGTCG GGATTCCCCA 
CTGCAGGGCA GTGGGCAGCA AAACAGCCAA GCAGGTCAGA GAAACTCCAC CAGCAGCATC 
GAGCCCAGGC TGCTATGGGA GAGAGTGGAG AAGCTGGTGC CCAGGCCTGG CAGTGGCAGC 
TCCTCGGGGT CCAGCAACTC AGGATCCCAG CCCGGCTCCC ACCCTGGGTC TCAGAGCGGC 
TCCGGGGAGC GCTTCAGAGT GAGATCGTCA TCCAAATCTG AGGGCTCGCC ATCTCAGCGC 
CTTGAAAATG CAGCAAAAAA GCCTGAAGAT AAGAAGGAAG TTTTTAGACC TCTCAAGCCC 
GCTGGCGAAG TGGACCTGAC GGCCCTGGCC AGAGAGCTCC GAGCGGCGGA AGACGTGCGG 
CCGCCGCACA AGGTGACAGA CTACTCGTCG TCCAGCGAGG AGTCCGGGAC AACGGATGAG 
GAGGACGACG ACGTGGAGCA GGAAGGCCCC GAGGAGCCCA CCTCGGGGCC GGAGGACGCC 
CGAGCCGCGT CATCTCTGAA CTTGAGCAAC GGTGAGACGG AGTCCGTGAA AACGATGATC 
GTCCACGACG ACGTGGAGAG TGAACCAGCC ATGACCCCGT CCAAGGAGGG CACCCTGATT 
GTCCGCCAGA CTCAGTCCGC TAGTAGCACA CTCCAGAAAC ACAAATCTTC CTCCTCCTTT 
ACACCTTTTA TAGACCCCAG ATTACTACAG ATTTCTCCAT CTAGTGGGAC AACAGTGACT 
TCTGTGGTGG GATTTTCCTG TGATGGAATG AGATCGGAGG CCATAAGGCA AGATCCTACC 
CGGAAGGGCT CAGTGGTCAA TGTGAATCCC ACCAACACTA GGCCACAGAG CGACACCCCA 
GAGATTCGCA AATATAAGAA GAGGTTTAAC TCCGAGATTC TGTGCGCTGC CTTGTGGGGA 
GTGAACTTGT TGGTGGGTAC AGAGAGTGGC CTGATGCTGC TGGACAGAAG TGGCCAAGGG 
AAGGTGTATC CTCTGATCAA CAGAAGACGA TTTCAACAGA TGGACGTCCT GGAAGGCTTG 
AATGTCTTGG TGACAATATC TGGCAAAAAG GATAAGTTAC GTGTCTACTA TTTGTCCTGG 
TTAAGAAATA AAATACTTCA CAACGATCCG GAAGTTGAGA AGAAGCAGGG TTGGACGACC 
GTGGGAGATT TGGAAGGATG CGTCCACTAT AAAGTCGTAA AATATGAAAG AATCAAGTTT 
CTGGTCATTG CTTTGAAGAG TTCTGTGGAA GTGTATGCGT GGGCACCCAA GCCATATCAC 
AAATTCATGG CCTTTAAGTC GTTTGGAGAT TTGGTGCATA AGCCATTACT GGTGGATCTC 
ACTGTTGAGG AAGGCCAGAG GTTGAAAGTG ATCTATGGAT CCTGTGCTGG ATTCCATGCT 
GTTGACGTGG ATTCAGGATC AGTCTATGAC ATTTATCTAC CAACACACAT CCAGTGCAGC 
ATCAAACCCC ACGCAATCAT CATCCTCCCC AACACGGATG GAATGGAGCT TCTGGTGTGC 
TATGAAGACG AAGGGGTCTA TGTGAACACC TATGGAAGGA TTACCAAGGA TGTGGTTCTG 
CAGTGGGGAG AGATGCCAAC GTCTGTAGCG TATATTCGAT CCAATCAGAC GATGGGCTGG 
GGAGAAAAGG CCATAGAGAT CCGGTCTGTG GAGACGGGCC ACTTGGACGG TGTGTTTATG 
CACAAAAGGG CTCAAAGACT GAAATTCTTA TGTGAACGCA ACGACAAGGT GTTCTTTGCC 
TCGGTCCGGT CCGGGGGCAG CAGCCAGGTG TACTTCATGA CGCTGGGCAG GACCTCTCTT 
CTGAGCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_019931231.1 ) cDNA ORF clone, Tursiops truncatus(common bottlenose dolphin) -> XP_019786790.1 Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X14, mRNA.

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CloneID	OTu39190
Clone ID Related Accession (Same CDS sequence)	XM_019931231.1
Accession Version	XM_019931231.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Tursiops truncatus(common bottlenose dolphin)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017842906.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tursiops truncatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
CCGGCTGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
AAGGGTCGAC ACGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA CGTTACTGAG 
GATGAAGAGG AGGAAATCAA GCTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGCGGGGCC GGGTCCATCA CAGACCTCGT GAAGAACACC 
AAGGGCAACA CGCTCAAGGA GGACTGGATA GCCTACATCT CCAGGGAGAT CCTGAGGGGA 
CTGGCGCATC TCCACATCCA TCACGTGATT CACCGGGACA TCAAGGGCCA GAATGTATTG 
CTGACCGAGA ACGCAGAGGT GAAGCTTGTG GATTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACCGTGGGGA GGAGAAACAC CTTCATAGGC ACCCCGTACT GGATGGCTCC CGAGGTCATC 
GCCTGTGACG AGAACCCCGA CGCCACCTAT GATTACAGGA GTGACCTGTG GTCCTGCGGC 
ATCACAGCCA TTGAGATGGC AGAAGGCGCT CCCCCTCTCT GTGACATGCA TCCCATGAGA 
GCACTGTTTC TCATCCCCAG AAACCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCAAAA 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACA 
GAGCAGCTTC TGAAACATCC TTTTATCAGG GACCAGCCGA ATGAAAGGCA GGTTAGGATC 
CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACCGAG 
TACGAGTACA GCGGGAGTGA GGAGGAGGAG GAGGAGGCGC CGGAGCAGGA GGGGGAGCCC 
AGCTCCATCG TCAACGTGCC TGGCGAGTCC ACGCTGCGCC GGGACTTCCT GAGGCTGCAG 
CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGCGGCAGC AGCTCCTGCA GGAGCAGCAG 
CTCAGGGAGC AGGAAGAATA CAAGAGGCAG CTGCTGGCGG AGAGACAGAA GCGGATCGAG 
CAGCAGAAGG AGCAGCGGCG ACGGCTGGAA GAGCAACAAA GGAGAGAGCG GGAGGCCAGG 
AGGCAGCAGG AGCGCGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
CTGGAGAGAC GGCGGCAGGA GGAAGAGGAG CGCAGGCGGG CGGAGGAGGA CAAGAGGAGA 
GTCGACAGGG AGCAGGAGTA TATCAGGCGG CAGCTGGAAG AGGAGCAGCG GCACCTGGAA 
CTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCATGAGCC CCGGAGGCCG 
CCCCCGCCGC CGGACAGGAG CAAGGCCGGC CTGCACGGCG CCGAGCCCAA GGCCCAGCAC 
GAGCCCGCCG ACCGGGCCCG CGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCAGATCA 
GAATCTTTTT CCAATGGCAA CTCCGAGTCT GTGCACCCTG CCCTGCAGAG ACCAGCCGAG 
CCACAGGTTC CTGTGAGAAC GACGTCCCGG TCCCCCGTCC TGTCCCGTCG GGATTCCCCA 
CTGCAGGGCA GTGGGCAGCA AAACAGCCAA GCAGGTCAGA GAAACTCCAC CAGCAGCATC 
GAGCCCAGGC TGCTATGGGA GAGAGTGGAG AAGCTGGTGC CCAGGCCTGG CAGTGGCAGC 
TCCTCGGGGT CCAGCAACTC AGGATCCCAG CCCGGCTCCC ACCCTGGGTC TCAGAGCGGC 
TCCGGGGAGC GCTTCAGAGT GAGATCGTCA TCCAAATCTG AGGGCTCGCC ATCTCAGCGC 
CTTGAAAATG CAGCAAAAAA GCCTGAAGAT AAGAAGGAAG TTTTTAGACC TCTCAAGCCC 
GCTGACCTGA CGGCCCTGGC CAGAGAGCTC CGAGCGGCGG AAGACGTGCG GCCGCCGCAC 
AAGGTGACAG ACTACTCGTC GTCCAGCGAG GAGTCCGGGA CAACGGATGA GGAGGACGAC 
GACGTGGAGC AGGAAGGCCC CGAGGAGCCC ACCTCGGGGC CGGAGGACGC CCGAGCCGCG 
TCATCTCTGA ACTTGAGCAA CGGTGAGACG GAGTCCGTGA AAACGATGAT CGTCCACGAC 
GACGTGGAGA GTGAACCAGC CATGACCCCG TCCAAGGAGG GCACCCTGAT TGTCCGCCAG 
ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA CACAAATCTT CCTCCTCCTT TACACCTTTT 
ATAGACCCCA GATTACTACA GATTTCTCCA TCTAGTGGGA CAACAGTGAC TTCTGTGGTG 
GGATTTTCCT GTGATGGAAT GAGATCGGAG GCCATAAGGC AAGATCCTAC CCGGAAGGGC 
TCAGTGGTCA ATGTGAATCC CACCAACACT AGGCCACAGA GCGACACCCC AGAGATTCGC 
AAATATAAGA AGAGGTTTAA CTCCGAGATT CTGTGCGCTG CCTTGTGGGG AGTGAACTTG 
TTGGTGGGTA CAGAGAGTGG CCTGATGCTG CTGGACAGAA GTGGCCAAGG GAAGGTGTAT 
CCTCTGATCA ACAGAAGACG ATTTCAACAG ATGGACGTCC TGGAAGGCTT GAATGTCTTG 
GTGACAATAT CTGGCAAAAA GGATAAGTTA CGTGTCTACT ATTTGTCCTG GTTAAGAAAT 
AAAATACTTC ACAACGATCC GGAAGTTGAG AAGAAGCAGG GTTGGACGAC CGTGGGAGAT 
TTGGAAGGAT GCGTCCACTA TAAAGTCGTA AAATATGAAA GAATCAAGTT TCTGGTCATT 
GCTTTGAAGA GTTCTGTGGA AGTGTATGCG TGGGCACCCA AGCCATATCA CAAATTCATG 
GCCTTTAAGT CGTTTGGAGA TTTGGTGCAT AAGCCATTAC TGGTGGATCT CACTGTTGAG 
GAAGGCCAGA GGTTGAAAGT GATCTATGGA TCCTGTGCTG GATTCCATGC TGTTGACGTG 
GATTCAGGAT CAGTCTATGA CATTTATCTA CCAACACACA TCCAGTGCAG CATCAAACCC 
CACGCAATCA TCATCCTCCC CAACACGGAT GGAATGGAGC TTCTGGTGTG CTATGAAGAC 
GAAGGGGTCT ATGTGAACAC CTATGGAAGG ATTACCAAGG ATGTGGTTCT GCAGTGGGGA 
GAGATGCCAA CGTCTGTAGC GTATATTCGA TCCAATCAGA CGATGGGCTG GGGAGAAAAG 
GCCATAGAGA TCCGGTCTGT GGAGACGGGC CACTTGGACG GTGTGTTTAT GCACAAAAGG 
GCTCAAAGAC TGAAATTCTT ATGTGAACGC AACGACAAGG TGTTCTTTGC CTCGGTCCGG 
TCCGGGGGCA GCAGCCAGGT GTACTTCATG ACGCTGGGCA GGACCTCTCT TCTGAGCTGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019786790.1
CDS	145..3747
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEAPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRQEEEERRRAEEDKRRVDREQEYIRRQLEEEQRHLELLQQQLLQEQAMLLHEPRRPPPPPDRSKAGLHGAEPKAQHEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPADLTALARELRAAEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGPEEPTSGPEDARAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRSEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGDLVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_019931231.1
Organism	Tursiops truncatus(common bottlenose dolphin)
Definition	Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019931231.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
CCGGCTGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
AAGGGTCGAC ACGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA CGTTACTGAG 
GATGAAGAGG AGGAAATCAA GCTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGCGGGGCC GGGTCCATCA CAGACCTCGT GAAGAACACC 
AAGGGCAACA CGCTCAAGGA GGACTGGATA GCCTACATCT CCAGGGAGAT CCTGAGGGGA 
CTGGCGCATC TCCACATCCA TCACGTGATT CACCGGGACA TCAAGGGCCA GAATGTATTG 
CTGACCGAGA ACGCAGAGGT GAAGCTTGTG GATTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACCGTGGGGA GGAGAAACAC CTTCATAGGC ACCCCGTACT GGATGGCTCC CGAGGTCATC 
GCCTGTGACG AGAACCCCGA CGCCACCTAT GATTACAGGA GTGACCTGTG GTCCTGCGGC 
ATCACAGCCA TTGAGATGGC AGAAGGCGCT CCCCCTCTCT GTGACATGCA TCCCATGAGA 
GCACTGTTTC TCATCCCCAG AAACCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCAAAA 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACA 
GAGCAGCTTC TGAAACATCC TTTTATCAGG GACCAGCCGA ATGAAAGGCA GGTTAGGATC 
CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACCGAG 
TACGAGTACA GCGGGAGTGA GGAGGAGGAG GAGGAGGCGC CGGAGCAGGA GGGGGAGCCC 
AGCTCCATCG TCAACGTGCC TGGCGAGTCC ACGCTGCGCC GGGACTTCCT GAGGCTGCAG 
CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGCGGCAGC AGCTCCTGCA GGAGCAGCAG 
CTCAGGGAGC AGGAAGAATA CAAGAGGCAG CTGCTGGCGG AGAGACAGAA GCGGATCGAG 
CAGCAGAAGG AGCAGCGGCG ACGGCTGGAA GAGCAACAAA GGAGAGAGCG GGAGGCCAGG 
AGGCAGCAGG AGCGCGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
CTGGAGAGAC GGCGGCAGGA GGAAGAGGAG CGCAGGCGGG CGGAGGAGGA CAAGAGGAGA 
GTCGACAGGG AGCAGGAGTA TATCAGGCGG CAGCTGGAAG AGGAGCAGCG GCACCTGGAA 
CTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCATGAGCC CCGGAGGCCG 
CCCCCGCCGC CGGACAGGAG CAAGGCCGGC CTGCACGGCG CCGAGCCCAA GGCCCAGCAC 
GAGCCCGCCG ACCGGGCCCG CGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCAGATCA 
GAATCTTTTT CCAATGGCAA CTCCGAGTCT GTGCACCCTG CCCTGCAGAG ACCAGCCGAG 
CCACAGGTTC CTGTGAGAAC GACGTCCCGG TCCCCCGTCC TGTCCCGTCG GGATTCCCCA 
CTGCAGGGCA GTGGGCAGCA AAACAGCCAA GCAGGTCAGA GAAACTCCAC CAGCAGCATC 
GAGCCCAGGC TGCTATGGGA GAGAGTGGAG AAGCTGGTGC CCAGGCCTGG CAGTGGCAGC 
TCCTCGGGGT CCAGCAACTC AGGATCCCAG CCCGGCTCCC ACCCTGGGTC TCAGAGCGGC 
TCCGGGGAGC GCTTCAGAGT GAGATCGTCA TCCAAATCTG AGGGCTCGCC ATCTCAGCGC 
CTTGAAAATG CAGCAAAAAA GCCTGAAGAT AAGAAGGAAG TTTTTAGACC TCTCAAGCCC 
GCTGACCTGA CGGCCCTGGC CAGAGAGCTC CGAGCGGCGG AAGACGTGCG GCCGCCGCAC 
AAGGTGACAG ACTACTCGTC GTCCAGCGAG GAGTCCGGGA CAACGGATGA GGAGGACGAC 
GACGTGGAGC AGGAAGGCCC CGAGGAGCCC ACCTCGGGGC CGGAGGACGC CCGAGCCGCG 
TCATCTCTGA ACTTGAGCAA CGGTGAGACG GAGTCCGTGA AAACGATGAT CGTCCACGAC 
GACGTGGAGA GTGAACCAGC CATGACCCCG TCCAAGGAGG GCACCCTGAT TGTCCGCCAG 
ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA CACAAATCTT CCTCCTCCTT TACACCTTTT 
ATAGACCCCA GATTACTACA GATTTCTCCA TCTAGTGGGA CAACAGTGAC TTCTGTGGTG 
GGATTTTCCT GTGATGGAAT GAGATCGGAG GCCATAAGGC AAGATCCTAC CCGGAAGGGC 
TCAGTGGTCA ATGTGAATCC CACCAACACT AGGCCACAGA GCGACACCCC AGAGATTCGC 
AAATATAAGA AGAGGTTTAA CTCCGAGATT CTGTGCGCTG CCTTGTGGGG AGTGAACTTG 
TTGGTGGGTA CAGAGAGTGG CCTGATGCTG CTGGACAGAA GTGGCCAAGG GAAGGTGTAT 
CCTCTGATCA ACAGAAGACG ATTTCAACAG ATGGACGTCC TGGAAGGCTT GAATGTCTTG 
GTGACAATAT CTGGCAAAAA GGATAAGTTA CGTGTCTACT ATTTGTCCTG GTTAAGAAAT 
AAAATACTTC ACAACGATCC GGAAGTTGAG AAGAAGCAGG GTTGGACGAC CGTGGGAGAT 
TTGGAAGGAT GCGTCCACTA TAAAGTCGTA AAATATGAAA GAATCAAGTT TCTGGTCATT 
GCTTTGAAGA GTTCTGTGGA AGTGTATGCG TGGGCACCCA AGCCATATCA CAAATTCATG 
GCCTTTAAGT CGTTTGGAGA TTTGGTGCAT AAGCCATTAC TGGTGGATCT CACTGTTGAG 
GAAGGCCAGA GGTTGAAAGT GATCTATGGA TCCTGTGCTG GATTCCATGC TGTTGACGTG 
GATTCAGGAT CAGTCTATGA CATTTATCTA CCAACACACA TCCAGTGCAG CATCAAACCC 
CACGCAATCA TCATCCTCCC CAACACGGAT GGAATGGAGC TTCTGGTGTG CTATGAAGAC 
GAAGGGGTCT ATGTGAACAC CTATGGAAGG ATTACCAAGG ATGTGGTTCT GCAGTGGGGA 
GAGATGCCAA CGTCTGTAGC GTATATTCGA TCCAATCAGA CGATGGGCTG GGGAGAAAAG 
GCCATAGAGA TCCGGTCTGT GGAGACGGGC CACTTGGACG GTGTGTTTAT GCACAAAAGG 
GCTCAAAGAC TGAAATTCTT ATGTGAACGC AACGACAAGG TGTTCTTTGC CTCGGTCCGG 
TCCGGGGGCA GCAGCCAGGT GTACTTCATG ACGCTGGGCA GGACCTCTCT TCTGAGCTGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_019931232.1 ) cDNA ORF clone, Tursiops truncatus(common bottlenose dolphin) -> XP_019786791.1 Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OTu39191
Clone ID Related Accession (Same CDS sequence)	XM_019931232.1
Accession Version	XM_019931232.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Tursiops truncatus(common bottlenose dolphin)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017842906.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tursiops truncatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
CCGGCTGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
AAGGGTCGAC ACGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA CGTTACTGAG 
GATGAAGAGG AGGAAATCAA GCTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGCGGGGCC GGGTCCATCA CAGACCTCGT GAAGAACACC 
AAGGGCAACA CGCTCAAGGA GGACTGGATA GCCTACATCT CCAGGGAGAT CCTGAGGGGA 
CTGGCGCATC TCCACATCCA TCACGTGATT CACCGGGACA TCAAGGGCCA GAATGTATTG 
CTGACCGAGA ACGCAGAGGT GAAGCTTGTG GATTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACCGTGGGGA GGAGAAACAC CTTCATAGGC ACCCCGTACT GGATGGCTCC CGAGGTCATC 
GCCTGTGACG AGAACCCCGA CGCCACCTAT GATTACAGGA GTGACCTGTG GTCCTGCGGC 
ATCACAGCCA TTGAGATGGC AGAAGGCGCT CCCCCTCTCT GTGACATGCA TCCCATGAGA 
GCACTGTTTC TCATCCCCAG AAACCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCAAAA 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACA 
GAGCAGCTTC TGAAACATCC TTTTATCAGG GACCAGCCGA ATGAAAGGCA GGTTAGGATC 
CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACCGAG 
TACGAGTACA GCGGGAGTGA GGAGGAGGAG GAGGAGGCGC CGGAGCAGGA GGGGGAGCCC 
AGCTCCATCG TCAACGTGCC TGGCGAGTCC ACGCTGCGCC GGGACTTCCT GAGGCTGCAG 
CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGCGGCAGC AGCTCCTGCA GGAGCAGCAG 
CTCAGGGAGC AGGAAGAATA CAAGAGGCAG CTGCTGGCGG AGAGACAGAA GCGGATCGAG 
CAGCAGAAGG AGCAGCGGCG ACGGCTGGAA GAGCAACAAA GGAGAGAGCG GGAGGCCAGG 
AGGCAGCAGG AGCGCGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
CTGGAGAGAC GGCGGCAGGA GGAAGAGGAG CGCAGGCGGG CGGAGGAGGA CAAGAGGAGA 
GTCGACAGGG AGCAGGAGTA TATCAGGCGG CAGCTGGAAG AGGAGCAGCG GCACCTGGAA 
CTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
CTGGAGGAGC AGCGGCAGAC GGAGCGGCTC CAGAGGCAGC TGCAGCAGGA GCAAGCCTAT 
CTCCTGTCTC TGCAGCATGA GCCCCGGAGG CCGCCCCCGC CGCCGGACAG GAGCAAGGCC 
GGCCTGCACG GCGCCGAGCC CAAGGCCCAG CACGAGCCCG CCGACCGGGC CCGCGAGGTT 
CCTGTGAGAA CGACGTCCCG GTCCCCCGTC CTGTCCCGTC GGGATTCCCC ACTGCAGGGC 
AGTGGGCAGC AAAACAGCCA AGCAGGTCAG AGAAACTCCA CCAGCAGCAT CGAGCCCAGG 
CTGCTATGGG AGAGAGTGGA GAAGCTGGTG CCCAGGCCTG GCAGTGGCAG CTCCTCGGGG 
TCCAGCAACT CAGGATCCCA GCCCGGCTCC CACCCTGGGT CTCAGAGCGG CTCCGGGGAG 
CGCTTCAGAG TGAGATCGTC ATCCAAATCT GAGGGCTCGC CATCTCAGCG CCTTGAAAAT 
GCAGCAAAAA AGCCTGAAGA TAAGAAGGAA GTTTTTAGAC CTCTCAAGCC CGCTGGCGAA 
GTGGACCTGA CGGCCCTGGC CAGAGAGCTC CGAGCGGCGG AAGACGTGCG GCCGCCGCAC 
AAGGTGACAG ACTACTCGTC GTCCAGCGAG GAGTCCGGGA CAACGGATGA GGAGGACGAC 
GACGTGGAGC AGGAAGGCCC CGAGGAGCCC ACCTCGGGGC CGGAGGACGC CCGAGCCGCG 
TCATCTCTGA ACTTGAGCAA CGGTGAGACG GAGTCCGTGA AAACGATGAT CGTCCACGAC 
GACGTGGAGA GTGAACCAGC CATGACCCCG TCCAAGGAGG GCACCCTGAT TGTCCGCCAG 
ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA CACAAATCTT CCTCCTCCTT TACACCTTTT 
ATAGACCCCA GATTACTACA GATTTCTCCA TCTAGTGGGA CAACAGTGAC TTCTGTGGTG 
GGATTTTCCT GTGATGGAAT GAGATCGGAG GCCATAAGGC AAGATCCTAC CCGGAAGGGC 
TCAGTGGTCA ATGTGAATCC CACCAACACT AGGCCACAGA GCGACACCCC AGAGATTCGC 
AAATATAAGA AGAGGTTTAA CTCCGAGATT CTGTGCGCTG CCTTGTGGGG AGTGAACTTG 
TTGGTGGGTA CAGAGAGTGG CCTGATGCTG CTGGACAGAA GTGGCCAAGG GAAGGTGTAT 
CCTCTGATCA ACAGAAGACG ATTTCAACAG ATGGACGTCC TGGAAGGCTT GAATGTCTTG 
GTGACAATAT CTGGCAAAAA GGATAAGTTA CGTGTCTACT ATTTGTCCTG GTTAAGAAAT 
AAAATACTTC ACAACGATCC GGAAGTTGAG AAGAAGCAGG GTTGGACGAC CGTGGGAGAT 
TTGGAAGGAT GCGTCCACTA TAAAGTCGTA AAATATGAAA GAATCAAGTT TCTGGTCATT 
GCTTTGAAGA GTTCTGTGGA AGTGTATGCG TGGGCACCCA AGCCATATCA CAAATTCATG 
GCCTTTAAGT CGTTTGGAGA TTTGGTGCAT AAGCCATTAC TGGTGGATCT CACTGTTGAG 
GAAGGCCAGA GGTTGAAAGT GATCTATGGA TCCTGTGCTG GATTCCATGC TGTTGACGTG 
GATTCAGGAT CAGTCTATGA CATTTATCTA CCAACACACA TCCAGTGCAG CATCAAACCC 
CACGCAATCA TCATCCTCCC CAACACGGAT GGAATGGAGC TTCTGGTGTG CTATGAAGAC 
GAAGGGGTCT ATGTGAACAC CTATGGAAGG ATTACCAAGG ATGTGGTTCT GCAGTGGGGA 
GAGATGCCAA CGTCTGTAGC GTATATTCGA TCCAATCAGA CGATGGGCTG GGGAGAAAAG 
GCCATAGAGA TCCGGTCTGT GGAGACGGGC CACTTGGACG GTGTGTTTAT GCACAAAAGG 
GCTCAAAGAC TGAAATTCTT ATGTGAACGC AACGACAAGG TGTTCTTTGC CTCGGTCCGG 
TCCGGGGGCA GCAGCCAGGT GTACTTCATG ACGCTGGGCA GGACCTCTCT TCTGAGCTGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019786791.1
CDS	143..3685
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEAPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRQEEEERRRAEEDKRRVDREQEYIRRQLEEEQRHLELLQQQLLQEQAMLLECRWRELEEQRQTERLQRQLQQEQAYLLSLQHEPRRPPPPPDRSKAGLHGAEPKAQHEPADRAREVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPAGEVDLTALARELRAAEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGPEEPTSGPEDARAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRSEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGDLVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_019931232.1
Organism	Tursiops truncatus(common bottlenose dolphin)
Definition	Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019931232.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
CCGGCTGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
AAGGGTCGAC ACGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA CGTTACTGAG 
GATGAAGAGG AGGAAATCAA GCTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGCGGGGCC GGGTCCATCA CAGACCTCGT GAAGAACACC 
AAGGGCAACA CGCTCAAGGA GGACTGGATA GCCTACATCT CCAGGGAGAT CCTGAGGGGA 
CTGGCGCATC TCCACATCCA TCACGTGATT CACCGGGACA TCAAGGGCCA GAATGTATTG 
CTGACCGAGA ACGCAGAGGT GAAGCTTGTG GATTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACCGTGGGGA GGAGAAACAC CTTCATAGGC ACCCCGTACT GGATGGCTCC CGAGGTCATC 
GCCTGTGACG AGAACCCCGA CGCCACCTAT GATTACAGGA GTGACCTGTG GTCCTGCGGC 
ATCACAGCCA TTGAGATGGC AGAAGGCGCT CCCCCTCTCT GTGACATGCA TCCCATGAGA 
GCACTGTTTC TCATCCCCAG AAACCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCAAAA 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACA 
GAGCAGCTTC TGAAACATCC TTTTATCAGG GACCAGCCGA ATGAAAGGCA GGTTAGGATC 
CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACCGAG 
TACGAGTACA GCGGGAGTGA GGAGGAGGAG GAGGAGGCGC CGGAGCAGGA GGGGGAGCCC 
AGCTCCATCG TCAACGTGCC TGGCGAGTCC ACGCTGCGCC GGGACTTCCT GAGGCTGCAG 
CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGCGGCAGC AGCTCCTGCA GGAGCAGCAG 
CTCAGGGAGC AGGAAGAATA CAAGAGGCAG CTGCTGGCGG AGAGACAGAA GCGGATCGAG 
CAGCAGAAGG AGCAGCGGCG ACGGCTGGAA GAGCAACAAA GGAGAGAGCG GGAGGCCAGG 
AGGCAGCAGG AGCGCGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
CTGGAGAGAC GGCGGCAGGA GGAAGAGGAG CGCAGGCGGG CGGAGGAGGA CAAGAGGAGA 
GTCGACAGGG AGCAGGAGTA TATCAGGCGG CAGCTGGAAG AGGAGCAGCG GCACCTGGAA 
CTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
CTGGAGGAGC AGCGGCAGAC GGAGCGGCTC CAGAGGCAGC TGCAGCAGGA GCAAGCCTAT 
CTCCTGTCTC TGCAGCATGA GCCCCGGAGG CCGCCCCCGC CGCCGGACAG GAGCAAGGCC 
GGCCTGCACG GCGCCGAGCC CAAGGCCCAG CACGAGCCCG CCGACCGGGC CCGCGAGGTT 
CCTGTGAGAA CGACGTCCCG GTCCCCCGTC CTGTCCCGTC GGGATTCCCC ACTGCAGGGC 
AGTGGGCAGC AAAACAGCCA AGCAGGTCAG AGAAACTCCA CCAGCAGCAT CGAGCCCAGG 
CTGCTATGGG AGAGAGTGGA GAAGCTGGTG CCCAGGCCTG GCAGTGGCAG CTCCTCGGGG 
TCCAGCAACT CAGGATCCCA GCCCGGCTCC CACCCTGGGT CTCAGAGCGG CTCCGGGGAG 
CGCTTCAGAG TGAGATCGTC ATCCAAATCT GAGGGCTCGC CATCTCAGCG CCTTGAAAAT 
GCAGCAAAAA AGCCTGAAGA TAAGAAGGAA GTTTTTAGAC CTCTCAAGCC CGCTGGCGAA 
GTGGACCTGA CGGCCCTGGC CAGAGAGCTC CGAGCGGCGG AAGACGTGCG GCCGCCGCAC 
AAGGTGACAG ACTACTCGTC GTCCAGCGAG GAGTCCGGGA CAACGGATGA GGAGGACGAC 
GACGTGGAGC AGGAAGGCCC CGAGGAGCCC ACCTCGGGGC CGGAGGACGC CCGAGCCGCG 
TCATCTCTGA ACTTGAGCAA CGGTGAGACG GAGTCCGTGA AAACGATGAT CGTCCACGAC 
GACGTGGAGA GTGAACCAGC CATGACCCCG TCCAAGGAGG GCACCCTGAT TGTCCGCCAG 
ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA CACAAATCTT CCTCCTCCTT TACACCTTTT 
ATAGACCCCA GATTACTACA GATTTCTCCA TCTAGTGGGA CAACAGTGAC TTCTGTGGTG 
GGATTTTCCT GTGATGGAAT GAGATCGGAG GCCATAAGGC AAGATCCTAC CCGGAAGGGC 
TCAGTGGTCA ATGTGAATCC CACCAACACT AGGCCACAGA GCGACACCCC AGAGATTCGC 
AAATATAAGA AGAGGTTTAA CTCCGAGATT CTGTGCGCTG CCTTGTGGGG AGTGAACTTG 
TTGGTGGGTA CAGAGAGTGG CCTGATGCTG CTGGACAGAA GTGGCCAAGG GAAGGTGTAT 
CCTCTGATCA ACAGAAGACG ATTTCAACAG ATGGACGTCC TGGAAGGCTT GAATGTCTTG 
GTGACAATAT CTGGCAAAAA GGATAAGTTA CGTGTCTACT ATTTGTCCTG GTTAAGAAAT 
AAAATACTTC ACAACGATCC GGAAGTTGAG AAGAAGCAGG GTTGGACGAC CGTGGGAGAT 
TTGGAAGGAT GCGTCCACTA TAAAGTCGTA AAATATGAAA GAATCAAGTT TCTGGTCATT 
GCTTTGAAGA GTTCTGTGGA AGTGTATGCG TGGGCACCCA AGCCATATCA CAAATTCATG 
GCCTTTAAGT CGTTTGGAGA TTTGGTGCAT AAGCCATTAC TGGTGGATCT CACTGTTGAG 
GAAGGCCAGA GGTTGAAAGT GATCTATGGA TCCTGTGCTG GATTCCATGC TGTTGACGTG 
GATTCAGGAT CAGTCTATGA CATTTATCTA CCAACACACA TCCAGTGCAG CATCAAACCC 
CACGCAATCA TCATCCTCCC CAACACGGAT GGAATGGAGC TTCTGGTGTG CTATGAAGAC 
GAAGGGGTCT ATGTGAACAC CTATGGAAGG ATTACCAAGG ATGTGGTTCT GCAGTGGGGA 
GAGATGCCAA CGTCTGTAGC GTATATTCGA TCCAATCAGA CGATGGGCTG GGGAGAAAAG 
GCCATAGAGA TCCGGTCTGT GGAGACGGGC CACTTGGACG GTGTGTTTAT GCACAAAAGG 
GCTCAAAGAC TGAAATTCTT ATGTGAACGC AACGACAAGG TGTTCTTTGC CTCGGTCCGG 
TCCGGGGGCA GCAGCCAGGT GTACTTCATG ACGCTGGGCA GGACCTCTCT TCTGAGCTGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_019931234.1 ) cDNA ORF clone, Tursiops truncatus(common bottlenose dolphin) -> XP_019786793.1 Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OTu39193
Clone ID Related Accession (Same CDS sequence)	XM_019931234.1
Accession Version	XM_019931234.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Tursiops truncatus(common bottlenose dolphin)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017842906.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tursiops truncatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
CCGGCTGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
AAGGGTCGAC ACGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA CGTTACTGAG 
GATGAAGAGG AGGAAATCAA GCTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGCGGGGCC GGGTCCATCA CAGACCTCGT GAAGAACACC 
AAGGGCAACA CGCTCAAGGA GGACTGGATA GCCTACATCT CCAGGGAGAT CCTGAGGGGA 
CTGGCGCATC TCCACATCCA TCACGTGATT CACCGGGACA TCAAGGGCCA GAATGTATTG 
CTGACCGAGA ACGCAGAGGT GAAGCTTGTG GATTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACCGTGGGGA GGAGAAACAC CTTCATAGGC ACCCCGTACT GGATGGCTCC CGAGGTCATC 
GCCTGTGACG AGAACCCCGA CGCCACCTAT GATTACAGGA GTGACCTGTG GTCCTGCGGC 
ATCACAGCCA TTGAGATGGC AGAAGGCGCT CCCCCTCTCT GTGACATGCA TCCCATGAGA 
GCACTGTTTC TCATCCCCAG AAACCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCAAAA 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACA 
GAGCAGCTTC TGAAACATCC TTTTATCAGG GACCAGCCGA ATGAAAGGCA GGTTAGGATC 
CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACCGAG 
TACGAGTACA GCGGGAGTGA GGAGGAGGAG GAGGAGGCGC CGGAGCAGGA GGGGGAGCCC 
AGCTCCATCG TCAACGTGCC TGGCGAGTCC ACGCTGCGCC GGGACTTCCT GAGGCTGCAG 
CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGCGGCAGC AGCTCCTGCA GGAGCAGCAG 
CTCAGGGAGC AGGAAGAATA CAAGAGGCAG CTGCTGGCGG AGAGACAGAA GCGGATCGAG 
CAGCAGAAGG AGCAGCGGCG ACGGCTGGAA GAGCAACAAA GGAGAGAGCG GGAGGCCAGG 
AGGCAGCAGG AGCGCGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
CTGGAGAGAC GGCGGCAGGA GGAAGAGGAG CGCAGGCGGG CGGAGGAGGA CAAGAGGAGA 
GTCGACAGGG AGCAGGAGTA TATCAGGCGG CAGCTGGAAG AGGAGCAGCG GCACCTGGAA 
CTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCATGAGCC CCGGAGGCCG 
CCCCCGCCGC CGGACAGGAG CAAGGCCGGC CTGCACGGCG CCGAGCCCAA GGCCCAGCAC 
GAGCCCGCCG ACCGGGCCCG CGAGGTTCCT GTGAGAACGA CGTCCCGGTC CCCCGTCCTG 
TCCCGTCGGG ATTCCCCACT GCAGGGCAGT GGGCAGCAAA ACAGCCAAGC AGGTCAGAGA 
AACTCCACCA GCATCGAGCC CAGGCTGCTA TGGGAGAGAG TGGAGAAGCT GGTGCCCAGG 
CCTGGCAGTG GCAGCTCCTC GGGGTCCAGC AACTCAGGAT CCCAGCCCGG CTCCCACCCT 
GGGTCTCAGA GCGGCTCCGG GGAGCGCTTC AGAGTGAGAT CGTCATCCAA ATCTGAGGGC 
TCGCCATCTC AGCGCCTTGA AAATGCAGCA AAAAAGCCTG AAGATAAGAA GGAAGTTTTT 
AGACCTCTCA AGCCCGCTGG CGAAGTGGAC CTGACGGCCC TGGCCAGAGA GCTCCGAGCG 
GCGGAAGACG TGCGGCCGCC GCACAAGGTG ACAGACTACT CGTCGTCCAG CGAGGAGTCC 
GGGACAACGG ATGAGGAGGA CGACGACGTG GAGCAGGAAG GCCCCGAGGA GCCCACCTCG 
GGGCCGGAGG ACGCCCGAGC CGCGTCATCT CTGAACTTGA GCAACGGTGA GACGGAGTCC 
GTGAAAACGA TGATCGTCCA CGACGACGTG GAGAGTGAAC CAGCCATGAC CCCGTCCAAG 
GAGGGCACCC TGATTGTCCG CCAGACTCAG TCCGCTAGTA GCACACTCCA GAAACACAAA 
TCTTCCTCCT CCTTTACACC TTTTATAGAC CCCAGATTAC TACAGATTTC TCCATCTAGT 
GGGACAACAG TGACTTCTGT GGTGGGATTT TCCTGTGATG GAATGAGATC GGAGGCCATA 
AGGCAAGATC CTACCCGGAA GGGCTCAGTG GTCAATGTGA ATCCCACCAA CACTAGGCCA 
CAGAGCGACA CCCCAGAGAT TCGCAAATAT AAGAAGAGGT TTAACTCCGA GATTCTGTGC 
GCTGCCTTGT GGGGAGTGAA CTTGTTGGTG GGTACAGAGA GTGGCCTGAT GCTGCTGGAC 
AGAAGTGGCC AAGGGAAGGT GTATCCTCTG ATCAACAGAA GACGATTTCA ACAGATGGAC 
GTCCTGGAAG GCTTGAATGT CTTGGTGACA ATATCTGGCA AAAAGGATAA GTTACGTGTC 
TACTATTTGT CCTGGTTAAG AAATAAAATA CTTCACAACG ATCCGGAAGT TGAGAAGAAG 
CAGGGTTGGA CGACCGTGGG AGATTTGGAA GGATGCGTCC ACTATAAAGT CGTAAAATAT 
GAAAGAATCA AGTTTCTGGT CATTGCTTTG AAGAGTTCTG TGGAAGTGTA TGCGTGGGCA 
CCCAAGCCAT ATCACAAATT CATGGCCTTT AAGTCGTTTG GAGATTTGGT GCATAAGCCA 
TTACTGGTGG ATCTCACTGT TGAGGAAGGC CAGAGGTTGA AAGTGATCTA TGGATCCTGT 
GCTGGATTCC ATGCTGTTGA CGTGGATTCA GGATCAGTCT ATGACATTTA TCTACCAACA 
CACGTAAGAA AGAACCCACA CTCTATGATC CAGTGCAGCA TCAAACCCCA CGCAATCATC 
ATCCTCCCCA ACACGGATGG AATGGAGCTT CTGGTGTGCT ATGAAGACGA AGGGGTCTAT 
GTGAACACCT ATGGAAGGAT TACCAAGGAT GTGGTTCTGC AGTGGGGAGA GATGCCAACG 
TCTGTAGCGT ATATTCGATC CAATCAGACG ATGGGCTGGG GAGAAAAGGC CATAGAGATC 
CGGTCTGTGG AGACGGGCCA CTTGGACGGT GTGTTTATGC ACAAAAGGGC TCAAAGACTG 
AAATTCTTAT GTGAACGCAA CGACAAGGTG TTCTTTGCCT CGGTCCGGTC CGGGGGCAGC 
AGCCAGGTGT ACTTCATGAC GCTGGGCAGG ACCTCTCTTC TGAGCTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019786793.1
CDS	147..3617
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEAPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRQEEEERRRAEEDKRRVDREQEYIRRQLEEEQRHLELLQQQLLQEQAMLLHEPRRPPPPPDRSKAGLHGAEPKAQHEPADRAREVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPAGEVDLTALARELRAAEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGPEEPTSGPEDARAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRSEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGDLVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_019931234.1
Organism	Tursiops truncatus(common bottlenose dolphin)
Definition	Tursiops truncatus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019931234.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
CCGGCTGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
AAGGGTCGAC ACGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA CGTTACTGAG 
GATGAAGAGG AGGAAATCAA GCTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGCGGGGCC GGGTCCATCA CAGACCTCGT GAAGAACACC 
AAGGGCAACA CGCTCAAGGA GGACTGGATA GCCTACATCT CCAGGGAGAT CCTGAGGGGA 
CTGGCGCATC TCCACATCCA TCACGTGATT CACCGGGACA TCAAGGGCCA GAATGTATTG 
CTGACCGAGA ACGCAGAGGT GAAGCTTGTG GATTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACCGTGGGGA GGAGAAACAC CTTCATAGGC ACCCCGTACT GGATGGCTCC CGAGGTCATC 
GCCTGTGACG AGAACCCCGA CGCCACCTAT GATTACAGGA GTGACCTGTG GTCCTGCGGC 
ATCACAGCCA TTGAGATGGC AGAAGGCGCT CCCCCTCTCT GTGACATGCA TCCCATGAGA 
GCACTGTTTC TCATCCCCAG AAACCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCAAAA 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACA 
GAGCAGCTTC TGAAACATCC TTTTATCAGG GACCAGCCGA ATGAAAGGCA GGTTAGGATC 
CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACCGAG 
TACGAGTACA GCGGGAGTGA GGAGGAGGAG GAGGAGGCGC CGGAGCAGGA GGGGGAGCCC 
AGCTCCATCG TCAACGTGCC TGGCGAGTCC ACGCTGCGCC GGGACTTCCT GAGGCTGCAG 
CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGCGGCAGC AGCTCCTGCA GGAGCAGCAG 
CTCAGGGAGC AGGAAGAATA CAAGAGGCAG CTGCTGGCGG AGAGACAGAA GCGGATCGAG 
CAGCAGAAGG AGCAGCGGCG ACGGCTGGAA GAGCAACAAA GGAGAGAGCG GGAGGCCAGG 
AGGCAGCAGG AGCGCGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
CTGGAGAGAC GGCGGCAGGA GGAAGAGGAG CGCAGGCGGG CGGAGGAGGA CAAGAGGAGA 
GTCGACAGGG AGCAGGAGTA TATCAGGCGG CAGCTGGAAG AGGAGCAGCG GCACCTGGAA 
CTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCATGAGCC CCGGAGGCCG 
CCCCCGCCGC CGGACAGGAG CAAGGCCGGC CTGCACGGCG CCGAGCCCAA GGCCCAGCAC 
GAGCCCGCCG ACCGGGCCCG CGAGGTTCCT GTGAGAACGA CGTCCCGGTC CCCCGTCCTG 
TCCCGTCGGG ATTCCCCACT GCAGGGCAGT GGGCAGCAAA ACAGCCAAGC AGGTCAGAGA 
AACTCCACCA GCATCGAGCC CAGGCTGCTA TGGGAGAGAG TGGAGAAGCT GGTGCCCAGG 
CCTGGCAGTG GCAGCTCCTC GGGGTCCAGC AACTCAGGAT CCCAGCCCGG CTCCCACCCT 
GGGTCTCAGA GCGGCTCCGG GGAGCGCTTC AGAGTGAGAT CGTCATCCAA ATCTGAGGGC 
TCGCCATCTC AGCGCCTTGA AAATGCAGCA AAAAAGCCTG AAGATAAGAA GGAAGTTTTT 
AGACCTCTCA AGCCCGCTGG CGAAGTGGAC CTGACGGCCC TGGCCAGAGA GCTCCGAGCG 
GCGGAAGACG TGCGGCCGCC GCACAAGGTG ACAGACTACT CGTCGTCCAG CGAGGAGTCC 
GGGACAACGG ATGAGGAGGA CGACGACGTG GAGCAGGAAG GCCCCGAGGA GCCCACCTCG 
GGGCCGGAGG ACGCCCGAGC CGCGTCATCT CTGAACTTGA GCAACGGTGA GACGGAGTCC 
GTGAAAACGA TGATCGTCCA CGACGACGTG GAGAGTGAAC CAGCCATGAC CCCGTCCAAG 
GAGGGCACCC TGATTGTCCG CCAGACTCAG TCCGCTAGTA GCACACTCCA GAAACACAAA 
TCTTCCTCCT CCTTTACACC TTTTATAGAC CCCAGATTAC TACAGATTTC TCCATCTAGT 
GGGACAACAG TGACTTCTGT GGTGGGATTT TCCTGTGATG GAATGAGATC GGAGGCCATA 
AGGCAAGATC CTACCCGGAA GGGCTCAGTG GTCAATGTGA ATCCCACCAA CACTAGGCCA 
CAGAGCGACA CCCCAGAGAT TCGCAAATAT AAGAAGAGGT TTAACTCCGA GATTCTGTGC 
GCTGCCTTGT GGGGAGTGAA CTTGTTGGTG GGTACAGAGA GTGGCCTGAT GCTGCTGGAC 
AGAAGTGGCC AAGGGAAGGT GTATCCTCTG ATCAACAGAA GACGATTTCA ACAGATGGAC 
GTCCTGGAAG GCTTGAATGT CTTGGTGACA ATATCTGGCA AAAAGGATAA GTTACGTGTC 
TACTATTTGT CCTGGTTAAG AAATAAAATA CTTCACAACG ATCCGGAAGT TGAGAAGAAG 
CAGGGTTGGA CGACCGTGGG AGATTTGGAA GGATGCGTCC ACTATAAAGT CGTAAAATAT 
GAAAGAATCA AGTTTCTGGT CATTGCTTTG AAGAGTTCTG TGGAAGTGTA TGCGTGGGCA 
CCCAAGCCAT ATCACAAATT CATGGCCTTT AAGTCGTTTG GAGATTTGGT GCATAAGCCA 
TTACTGGTGG ATCTCACTGT TGAGGAAGGC CAGAGGTTGA AAGTGATCTA TGGATCCTGT 
GCTGGATTCC ATGCTGTTGA CGTGGATTCA GGATCAGTCT ATGACATTTA TCTACCAACA 
CACGTAAGAA AGAACCCACA CTCTATGATC CAGTGCAGCA TCAAACCCCA CGCAATCATC 
ATCCTCCCCA ACACGGATGG AATGGAGCTT CTGGTGTGCT ATGAAGACGA AGGGGTCTAT 
GTGAACACCT ATGGAAGGAT TACCAAGGAT GTGGTTCTGC AGTGGGGAGA GATGCCAACG 
TCTGTAGCGT ATATTCGATC CAATCAGACG ATGGGCTGGG GAGAAAAGGC CATAGAGATC 
CGGTCTGTGG AGACGGGCCA CTTGGACGGT GTGTTTATGC ACAAAAGGGC TCAAAGACTG 
AAATTCTTAT GTGAACGCAA CGACAAGGTG TTCTTTGCCT CGGTCCGGTC CGGGGGCAGC 
AGCCAGGTGT ACTTCATGAC GCTGGGCAGG ACCTCTCTTC TGAGCTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MAP4K4
Entrez Gene ID	101334207
Full Name	mitogen-activated protein kinase kinase kinase kinase 4
Gene Type	protein-coding
Organism	Tursiops truncatus(common bottlenose dolphin)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

MAP4K4 cDNA ORF clone, Tursiops truncatus(common bottlenose dolphin)

Gene

Clones

gRNAs

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