LOC101327442 cDNA ORF clone, Tursiops truncatus(common bottlenose dolphin)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101327442
Entrez Gene ID	101327442
Full Name	E3 ubiquitin-protein ligase HECW2
Synonyms	HECW2
Gene Type	protein-coding
Organism	Tursiops truncatus(common bottlenose dolphin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019939237.1	XP_019794796.1	E3 ubiquitin-protein ligase HECW2 isoform X7
XM_019939235.1	XP_019794794.1	E3 ubiquitin-protein ligase HECW2 isoform X5
XM_019939231.1	XP_019794790.1	E3 ubiquitin-protein ligase HECW2 isoform X1
XM_019939234.1	XP_019794793.1	E3 ubiquitin-protein ligase HECW2 isoform X4
XM_019939232.1	XP_019794791.1	E3 ubiquitin-protein ligase HECW2 isoform X2
XM_019939233.1	XP_019794792.1	E3 ubiquitin-protein ligase HECW2 isoform X3
XM_019939236.1	XP_019794795.1	E3 ubiquitin-protein ligase HECW2 isoform X6

Pathways from BioSystems
Gene Ontology

The following LOC101327442 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101327442 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTu45677	XM_019939232.1 Latest version!	Tursiops truncatus E3 ubiquitin-protein ligase HECW2 (LOC101327442), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OTu45678	XM_019939233.1 Latest version!	Tursiops truncatus E3 ubiquitin-protein ligase HECW2 (LOC101327442), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OTu45679	XM_019939234.1 Latest version!	Tursiops truncatus E3 ubiquitin-protein ligase HECW2 (LOC101327442), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OTu45680	XM_019939235.1 Latest version!	Tursiops truncatus E3 ubiquitin-protein ligase HECW2 (LOC101327442), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OTu45681	XM_019939236.1 Latest version!	Tursiops truncatus E3 ubiquitin-protein ligase HECW2 (LOC101327442), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OTu45682	XM_019939237.1 Latest version!	Tursiops truncatus E3 ubiquitin-protein ligase HECW2 (LOC101327442), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OTu45676	XM_019939231.1 Latest version!	Tursiops truncatus E3 ubiquitin-protein ligase HECW2 (LOC101327442), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101327442 ( XM_019939232.1 ) cDNA ORF clone, Tursiops truncatus(common bottlenose dolphin) -> XP_019794791.1 Tursiops truncatus E3 ubiquitin-protein ligase HECW2 (LOC101327442), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTu45677
Clone ID Related Accession (Same CDS sequence)	XM_019939232.1
Accession Version	XM_019939232.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Tursiops truncatus(common bottlenose dolphin)
Product	E3 ubiquitin-protein ligase HECW2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017843453.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tursiops truncatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCTG CGGAGATAAA AATCTGTTTC AAATACTACC ACGGCATTAG TGGAGCCCTG 
CGAGCCACGA CCCCCTGCAT CACGGTGAAG AACCCCGCTG TGATGATGGG GGCAGAAGGC 
ATGGAAGGAG GTGCTTCAGG AAGTCAGCAC TCTCGAAAAC TCGTTAGCTT TACGTTGTCT 
GATCTTCGGG CAGTTGGACT AAAGAAAGGG ATGTTCTTCA ATCCTGACCC TTATCTTAAG 
ATGTCGATTC AGCCAGGGAA GAAGAGCAGT TTCCCCACTT GTGCCCACCA TGGGCAGGAG 
AGGAGGTCTA CTATCATCAG TAACACCACA AATCCAATTT GGCACCGAGA GAAATATTCC 
TTTTTCGCAC TCCTTACTGA TGTCTTAGAA ATTGAAATTA AAGACAAATT TGCCAAGAGC 
CGACCTATCA TCAAGCGTTT TCTGGGGAAA CTAACCATTC CAGTCCAGAG GCTTTTGGAG 
CGACAAGCCA TCGGTGACCA GATGCTCAGC TACAACCTCG GCAGAAGGCT CCCAGCTGAC 
CACGTGAGTG GGTACCTCCA GTTCAGAGTG GAGGTCACAT CTTCTGTGCA TGAAGATGCT 
TCTCCAGAAG CTGTTGGCAC AATACTTGGA GTCAATACTG TGAATGGAGA CCTAGGAAGC 
CCTTCTGATG AGGAGGACAT GCCAGGGGGC CATCATGACA GCACAGTTTG TTCCAATGGA 
CCAGTTTCTG AGGAAAGTGC TAATGGGACC CCCAAGCATT CTTTCAGGAC TAGCTCTACT 
CTGGAAATAG ACTCAGAAGA ATTCACCTCT ACTTCTTCAA GGACCTCACC TCCCAGAGGA 
CGTCAGGATT CACTCAATGA TTACTTAGAT GCTATTGAAC ACAATGGCCA TTCCAGGTCG 
GGTGCAGCTA CTTGTTCGGA AAGGTCCATG GGAGCCTCTC CGAAACTAAG GAGTAGTTTT 
CCCACCGACA CAAGACTCAA TGCAATGCTT CATATCGACT CAGATGAAGA AGACCACGAG 
TTCCAGCAAG ACCTGGGTTA TCCATCCTCC TTGGAGGAGG AAGGAGGGTT GATAATGTTT 
AGCAGGGCGT CAAGAACTGA TGATGGGAGC CTGACATCTC AGATAAAGCC AGAAGACAAC 
ACGGTGGAGA ATGAGGAAGC CTCCACCAAT GAAGCTGCTT CCTTCGAGGA CAAGCCAGAA 
AGTCTTCCAG AGCTTGCGGA GAGCTCCTTC CCCTCAGGCC CAGTCCCTGA TGAAAGCGAG 
AATGCCCCGG AGTGTCAGCC GAGTGCTGAC CAGGGGAGCA CTGAATTGTG CGGCGCCCAG 
GAGGTAGATC AGCCCACAAG TGGGGCAGAC ACGGGCACTT CTGACACGTC AGGAGGAAGC 
CGAAGAGCCG TCAGTGAGAT GGAGTCCCTT GATCAGGGGT CTGAGCCTTC CCAGGTGTCC 
TCGGAAACAG AGCCCAGTGA CCCCGCCAGG ACAGAGAGCG TGAGCGAGGC CAGCACCAGG 
CCTGAGGGAG AGAGTGACCT GGACGGGGCC GACAGCTCCT GCAATGAGAG TGTCACCACG 
CAGCTGTCCT CGGTGGAGAC ACGGTGCTCA TCTCTTGAGA GTGCGCGGTT TCCCGAAACT 
CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT GGAGCCTGTG CGGCAGAGCC CACCAGCAGT 
GGCCCTGCTG AAGGGTCACA GGGTTCCATA TGCACTCCTG GTTCTTTACC TGTAGTGCAG 
GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG GCAGAATCGG CTACTTCACC TGACCAGGAG 
GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG AGCCTGGAGG GAGCTGCTGC TGCTACTGAG 
AGCCCGCCCC AAGAAGAGCG TGATGCGGGG CATGCCCAAG GGGCTTGTGA AGGGGCCACT 
GCCCAGGAGG AGGGCGCGAC CGGAGGTTCT CAAGCCAACG GCCACCAGCC TTTGCGATCA 
CTGCCTTCAG TGCGCCAGGA TGTTAGCCGG TACCAGAGGG TGGACGAGGC TCTCCCACCA 
AACTGGGAGG CACGCATTGA CAGCCATGGC AGGATCTTCT ACGTGGATCA CGTAAACAGA 
ACCACGACTT GGCAGCGGCC CACAGCTCCC CCTGCCCCGC AGGTGCTGCA GAGGTCCAAC 
TCCATACAGC AGATGGAGCA GCTGAACCGG CGATACCAGA GTATCCGCAG AACCATGACA 
AACGAGAGGC CTGAGGAAAA CACCAATGCG ATTGATGTGG CAGGGGAGGA AGCCGACTTT 
CACCAAGCCA GTGCAGATTT CCGACGGGAG AACGTCCCGC CCCACTCTAC CTCCAGATCC 
AGGCTCACGT TGCTGTTACA GTCTCCCCCC GTGAAGTTCC TCATCAGCCC AGAGTTCTTC 
ACTGTGCTGC ATTCTAACCC TAGTGCCTAC CGCATGTTTA CAAACAACAC GTGTTTGAAG 
CACATGATCA CCAAAGTCCG GCGGGACACC CACCACTTTG AACGCTACCA GCATAACCGG 
GACCTTGTGG GATTCCTCAA CATGTTTGCA AACAAACAAC TGGAGCTGCC GAGGGGCTGG 
GAAATGAAAC ATGATCATCA GGGCAAGGCA TTTTTTGTCG ACCACAACTC GCGTACCACC 
ACCTTCATCG ATCCCCGGCT CCCGCTTCAG AGCAGCAGAC CCACGAGCGC GCTGGTGCAT 
CGACAGCACC TGACCAGGCA GCGCAGCCAC AGCGCAGGCG AGGTAGGAGA AGATTCTCGA 
CATGCAGGAC CACCAGTTCT TCCCAGGCCA TCAACTTACA ATGACAAGAT TGTTGCATTT 
CTGCGCCAGC CCAACATCTT TGAAATTCTA CAGGAGCGTC AACCTGATCT TACCAGGAAT 
CACTCACTCA GGGAGAAGAT CCAATTTATC CGAACTGAAG GGACCCCAGG ATTGGTGCGT 
CTTTCAAGTG ATGCCGACCT TGTTATGTTG CTGAGTTTAT TTGAAGAAGA GATAATGTCA 
TATGTGCCTC CACATGCCTT ACTCCATCCC AGCTACTGTC AGTCCCCACG TGGCTCTCCT 
GTGTCCTCTC CCCAGAACTC ACCAGGTACT CAGCGTGCCA ATGCCCGTGC TCCAGCCCCT 
TACAAGCGAG ACTTTGAAGC CAAACTAAGA AACTTTTACA GGAAGTTAGA GACTAAAGGA 
TATGGACAAG GCCCAGGGAA ATTAAAGTTA ATTATCAGAA GAGATCACTT GCTCGAAGAT 
GCTTTTAATC AGATTATGGG CTACTCCAGA AAAGACCTGC AGAGAAATAA GCTGTATGTC 
ACATTTGTTG GGGAGGAAGG GCTGGATTAC AGTGGACCTT CCAGAGAGTT TTTCTTCCTG 
GTATCCAGAG AACTCTTTAA CCCATATTAT GGCTTATTTG AATATTCAGC CAATGACACA 
TACACAGTAC AAATAAGTCC CATGTCTGCT TTTGTAGACA ATCACCATGA ATGGTTCCGG 
TTCAGTGGTA GGATCCTTGG TCTTGCACTA ATTCACCAGT ATTTGTTGGA TGCCTTCTTC 
ACACGGCCCT TTTATAAGGC TCTTCTCAGA ATTCTCTGTG ACCTGAGTGA TCTAGAATAC 
CTTGATGAAG AATTTCACCA GAGCCTGCAG TGGATGAAAG ACAATGACAT CCATGACATC 
CTGGACCTCA CGTTCACTGT GAACGAAGAA GTTTTTGGGC AGATTACCGA ACGAGAATTA 
AAACCAGGGG GTGCTAACAT CCCAGTCACG GAGAAGAATA AGAAGGAGTA CATCGAGAGG 
ATGGTGAAAT GGAGGATTGA GAGGGGTGTT GTGCAGCAAA CAGAGAGCTT AGTGCGTGGC 
TTTTATGAGG TCGTGGATGC CAGGCTGGTA TCTGTTTTTG ATGCGAGAGA ACTAGAACTG 
GTCATCGCAG GCACAGCTGA AATAGACCTA AGTGATTGGA GGAACAACAC AGAATATAGA 
GGAGGATATC ATGACAATCA TATTGTAATT CGGTGGTTCT GGGCTGCAGT GGAAAGATTC 
AACAATGAAC AACGACTAAG GTTGTTACAG TTTGTTACAG GCACATCCAG CATTCCCTAT 
GAAGGATTTG CTTCTCTCCG AGGGAGTAAC GGCCCAAGAA GATTCTGTGT GGAGAAATGG 
GGGAAAATCA CCGCTCTTCC CAGTACAGAG AGCATTGTCT ACTGGAGCTA TCAGCTTGGA 
AATTGGAAAG TAACTGAGGA GCTGGTATCA CTTTCTTTAA TTAAAAAAAA ATCCAGAACA 
AGTGATGGAC ACTGGGAGGA TCGCTTGGTG ACACCACCAC TTTTCCGTAT TGTGCCTGCC 
TTGGGACGTC CCCCCTTGGT CTCCCAGTTC CCCACCACCT GCTGGTCCCA CGGCAGTCTG 
CTCCATCATT TAATCCCTGA TACAAGGCCC TCTTCCACCC TTTCCCTTTC TTTCCACTTA 
CTCAATTTAT ACTGTCTTAT AATAATATTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019794791.1
CDS	2488..6960
Translation	MEAAEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGSQHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFRVEVTSSVHEDASPEAVGTILGVNTVNGDLGSPSDEEDMPGGHHDSTVCSNGPVSEESANGTPKHSFRTSSTLEIDSEEFTSTSSRTSPPRGRQDSLNDYLDAIEHNGHSRSGAATCSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRTDDGSLTSQIKPEDNTVENEEASTNEAASFEDKPESLPELAESSFPSGPVPDESENAPECQPSADQGSTELCGAQEVDQPTSGADTGTSDTSGGSRRAVSEMESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLDGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQGSICTPGSLPVVQVPSGEEEGPGAESATSPDQEEVGEVWQRRGSLEGAAAATESPPQEERDAGHAQGACEGATAQEEGATGGSQANGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTNAIDVAGEEADFHQASADFRRENVPPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSTYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPSTESIVYWSYQLGNWKVTEELVSLSLIKKKSRTSDGHWEDRLVTPPLFRIVPALGRPPLVSQFPTTCWSHGSLLHHLIPDTRPSSTLSLSFHLLNLYCLIIIF

Target ORF information:

RefSeq Version	XM_019939232.1
Organism	Tursiops truncatus(common bottlenose dolphin)
Definition	Tursiops truncatus E3 ubiquitin-protein ligase HECW2 (LOC101327442), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019939232.1

ORF Insert Sequence:

ATGGAAGCTG CGGAGATAAA AATCTGTTTC AAATACTACC ACGGCATTAG TGGAGCCCTG 
CGAGCCACGA CCCCCTGCAT CACGGTGAAG AACCCCGCTG TGATGATGGG GGCAGAAGGC 
ATGGAAGGAG GTGCTTCAGG AAGTCAGCAC TCTCGAAAAC TCGTTAGCTT TACGTTGTCT 
GATCTTCGGG CAGTTGGACT AAAGAAAGGG ATGTTCTTCA ATCCTGACCC TTATCTTAAG 
ATGTCGATTC AGCCAGGGAA GAAGAGCAGT TTCCCCACTT GTGCCCACCA TGGGCAGGAG 
AGGAGGTCTA CTATCATCAG TAACACCACA AATCCAATTT GGCACCGAGA GAAATATTCC 
TTTTTCGCAC TCCTTACTGA TGTCTTAGAA ATTGAAATTA AAGACAAATT TGCCAAGAGC 
CGACCTATCA TCAAGCGTTT TCTGGGGAAA CTAACCATTC CAGTCCAGAG GCTTTTGGAG 
CGACAAGCCA TCGGTGACCA GATGCTCAGC TACAACCTCG GCAGAAGGCT CCCAGCTGAC 
CACGTGAGTG GGTACCTCCA GTTCAGAGTG GAGGTCACAT CTTCTGTGCA TGAAGATGCT 
TCTCCAGAAG CTGTTGGCAC AATACTTGGA GTCAATACTG TGAATGGAGA CCTAGGAAGC 
CCTTCTGATG AGGAGGACAT GCCAGGGGGC CATCATGACA GCACAGTTTG TTCCAATGGA 
CCAGTTTCTG AGGAAAGTGC TAATGGGACC CCCAAGCATT CTTTCAGGAC TAGCTCTACT 
CTGGAAATAG ACTCAGAAGA ATTCACCTCT ACTTCTTCAA GGACCTCACC TCCCAGAGGA 
CGTCAGGATT CACTCAATGA TTACTTAGAT GCTATTGAAC ACAATGGCCA TTCCAGGTCG 
GGTGCAGCTA CTTGTTCGGA AAGGTCCATG GGAGCCTCTC CGAAACTAAG GAGTAGTTTT 
CCCACCGACA CAAGACTCAA TGCAATGCTT CATATCGACT CAGATGAAGA AGACCACGAG 
TTCCAGCAAG ACCTGGGTTA TCCATCCTCC TTGGAGGAGG AAGGAGGGTT GATAATGTTT 
AGCAGGGCGT CAAGAACTGA TGATGGGAGC CTGACATCTC AGATAAAGCC AGAAGACAAC 
ACGGTGGAGA ATGAGGAAGC CTCCACCAAT GAAGCTGCTT CCTTCGAGGA CAAGCCAGAA 
AGTCTTCCAG AGCTTGCGGA GAGCTCCTTC CCCTCAGGCC CAGTCCCTGA TGAAAGCGAG 
AATGCCCCGG AGTGTCAGCC GAGTGCTGAC CAGGGGAGCA CTGAATTGTG CGGCGCCCAG 
GAGGTAGATC AGCCCACAAG TGGGGCAGAC ACGGGCACTT CTGACACGTC AGGAGGAAGC 
CGAAGAGCCG TCAGTGAGAT GGAGTCCCTT GATCAGGGGT CTGAGCCTTC CCAGGTGTCC 
TCGGAAACAG AGCCCAGTGA CCCCGCCAGG ACAGAGAGCG TGAGCGAGGC CAGCACCAGG 
CCTGAGGGAG AGAGTGACCT GGACGGGGCC GACAGCTCCT GCAATGAGAG TGTCACCACG 
CAGCTGTCCT CGGTGGAGAC ACGGTGCTCA TCTCTTGAGA GTGCGCGGTT TCCCGAAACT 
CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT GGAGCCTGTG CGGCAGAGCC CACCAGCAGT 
GGCCCTGCTG AAGGGTCACA GGGTTCCATA TGCACTCCTG GTTCTTTACC TGTAGTGCAG 
GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG GCAGAATCGG CTACTTCACC TGACCAGGAG 
GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG AGCCTGGAGG GAGCTGCTGC TGCTACTGAG 
AGCCCGCCCC AAGAAGAGCG TGATGCGGGG CATGCCCAAG GGGCTTGTGA AGGGGCCACT 
GCCCAGGAGG AGGGCGCGAC CGGAGGTTCT CAAGCCAACG GCCACCAGCC TTTGCGATCA 
CTGCCTTCAG TGCGCCAGGA TGTTAGCCGG TACCAGAGGG TGGACGAGGC TCTCCCACCA 
AACTGGGAGG CACGCATTGA CAGCCATGGC AGGATCTTCT ACGTGGATCA CGTAAACAGA 
ACCACGACTT GGCAGCGGCC CACAGCTCCC CCTGCCCCGC AGGTGCTGCA GAGGTCCAAC 
TCCATACAGC AGATGGAGCA GCTGAACCGG CGATACCAGA GTATCCGCAG AACCATGACA 
AACGAGAGGC CTGAGGAAAA CACCAATGCG ATTGATGTGG CAGGGGAGGA AGCCGACTTT 
CACCAAGCCA GTGCAGATTT CCGACGGGAG AACGTCCCGC CCCACTCTAC CTCCAGATCC 
AGGCTCACGT TGCTGTTACA GTCTCCCCCC GTGAAGTTCC TCATCAGCCC AGAGTTCTTC 
ACTGTGCTGC ATTCTAACCC TAGTGCCTAC CGCATGTTTA CAAACAACAC GTGTTTGAAG 
CACATGATCA CCAAAGTCCG GCGGGACACC CACCACTTTG AACGCTACCA GCATAACCGG 
GACCTTGTGG GATTCCTCAA CATGTTTGCA AACAAACAAC TGGAGCTGCC GAGGGGCTGG 
GAAATGAAAC ATGATCATCA GGGCAAGGCA TTTTTTGTCG ACCACAACTC GCGTACCACC 
ACCTTCATCG ATCCCCGGCT CCCGCTTCAG AGCAGCAGAC CCACGAGCGC GCTGGTGCAT 
CGACAGCACC TGACCAGGCA GCGCAGCCAC AGCGCAGGCG AGGTAGGAGA AGATTCTCGA 
CATGCAGGAC CACCAGTTCT TCCCAGGCCA TCAACTTACA ATGACAAGAT TGTTGCATTT 
CTGCGCCAGC CCAACATCTT TGAAATTCTA CAGGAGCGTC AACCTGATCT TACCAGGAAT 
CACTCACTCA GGGAGAAGAT CCAATTTATC CGAACTGAAG GGACCCCAGG ATTGGTGCGT 
CTTTCAAGTG ATGCCGACCT TGTTATGTTG CTGAGTTTAT TTGAAGAAGA GATAATGTCA 
TATGTGCCTC CACATGCCTT ACTCCATCCC AGCTACTGTC AGTCCCCACG TGGCTCTCCT 
GTGTCCTCTC CCCAGAACTC ACCAGGTACT CAGCGTGCCA ATGCCCGTGC TCCAGCCCCT 
TACAAGCGAG ACTTTGAAGC CAAACTAAGA AACTTTTACA GGAAGTTAGA GACTAAAGGA 
TATGGACAAG GCCCAGGGAA ATTAAAGTTA ATTATCAGAA GAGATCACTT GCTCGAAGAT 
GCTTTTAATC AGATTATGGG CTACTCCAGA AAAGACCTGC AGAGAAATAA GCTGTATGTC 
ACATTTGTTG GGGAGGAAGG GCTGGATTAC AGTGGACCTT CCAGAGAGTT TTTCTTCCTG 
GTATCCAGAG AACTCTTTAA CCCATATTAT GGCTTATTTG AATATTCAGC CAATGACACA 
TACACAGTAC AAATAAGTCC CATGTCTGCT TTTGTAGACA ATCACCATGA ATGGTTCCGG 
TTCAGTGGTA GGATCCTTGG TCTTGCACTA ATTCACCAGT ATTTGTTGGA TGCCTTCTTC 
ACACGGCCCT TTTATAAGGC TCTTCTCAGA ATTCTCTGTG ACCTGAGTGA TCTAGAATAC 
CTTGATGAAG AATTTCACCA GAGCCTGCAG TGGATGAAAG ACAATGACAT CCATGACATC 
CTGGACCTCA CGTTCACTGT GAACGAAGAA GTTTTTGGGC AGATTACCGA ACGAGAATTA 
AAACCAGGGG GTGCTAACAT CCCAGTCACG GAGAAGAATA AGAAGGAGTA CATCGAGAGG 
ATGGTGAAAT GGAGGATTGA GAGGGGTGTT GTGCAGCAAA CAGAGAGCTT AGTGCGTGGC 
TTTTATGAGG TCGTGGATGC CAGGCTGGTA TCTGTTTTTG ATGCGAGAGA ACTAGAACTG 
GTCATCGCAG GCACAGCTGA AATAGACCTA AGTGATTGGA GGAACAACAC AGAATATAGA 
GGAGGATATC ATGACAATCA TATTGTAATT CGGTGGTTCT GGGCTGCAGT GGAAAGATTC 
AACAATGAAC AACGACTAAG GTTGTTACAG TTTGTTACAG GCACATCCAG CATTCCCTAT 
GAAGGATTTG CTTCTCTCCG AGGGAGTAAC GGCCCAAGAA GATTCTGTGT GGAGAAATGG 
GGGAAAATCA CCGCTCTTCC CAGTACAGAG AGCATTGTCT ACTGGAGCTA TCAGCTTGGA 
AATTGGAAAG TAACTGAGGA GCTGGTATCA CTTTCTTTAA TTAAAAAAAA ATCCAGAACA 
AGTGATGGAC ACTGGGAGGA TCGCTTGGTG ACACCACCAC TTTTCCGTAT TGTGCCTGCC 
TTGGGACGTC CCCCCTTGGT CTCCCAGTTC CCCACCACCT GCTGGTCCCA CGGCAGTCTG 
CTCCATCATT TAATCCCTGA TACAAGGCCC TCTTCCACCC TTTCCCTTTC TTTCCACTTA 
CTCAATTTAT ACTGTCTTAT AATAATATTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101327442 ( XM_019939233.1 ) cDNA ORF clone, Tursiops truncatus(common bottlenose dolphin) -> XP_019794792.1 Tursiops truncatus E3 ubiquitin-protein ligase HECW2 (LOC101327442), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OTu45678
Clone ID Related Accession (Same CDS sequence)	XM_019939233.1
Accession Version	XM_019939233.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Tursiops truncatus(common bottlenose dolphin)
Product	E3 ubiquitin-protein ligase HECW2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017843453.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tursiops truncatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCTG CGGAGATAAA AATCTGTTTC AAATACTACC ACGGCATTAG TGGAGCCCTG 
CGAGCCACGA CCCCCTGCAT CACGGTGAAG AACCCCGCTG TGATGATGGG GGCAGAAGGC 
ATGGAAGGAG GTGCTTCAGG AAGTCAGCAC TCTCGAAAAC TCGTTAGCTT TACGTTGTCT 
GATCTTCGGG CAGTTGGACT AAAGAAAGGG ATGTTCTTCA ATCCTGACCC TTATCTTAAG 
ATGTCGATTC AGCCAGGGAA GAAGAGCAGT TTCCCCACTT GTGCCCACCA TGGGCAGGAG 
AGGAGGTCTA CTATCATCAG TAACACCACA AATCCAATTT GGCACCGAGA GAAATATTCC 
TTTTTCGCAC TCCTTACTGA TGTCTTAGAA ATTGAAATTA AAGACAAATT TGCCAAGAGC 
CGACCTATCA TCAAGCGTTT TCTGGGGAAA CTAACCATTC CAGTCCAGAG GCTTTTGGAG 
CGACAAGCCA TCGGTGACCA GATGCTCAGC TACAACCTCG GCAGAAGGCT CCCAGCTGAC 
CACGTGAGTG GGTACCTCCA GTTCAGAGTG GAGGTCACAT CTTCTGTGCA TGAAGATGCT 
TCTCCAGAAG CTGTTGGCAC AATACTTGGA GTCAATACTG TGAATGGAGA CCTAGGAAGC 
CCTTCTGATG AGGAGGACAT GCCAGGGGGC CATCATGACA GCACAGTTTG TTCCAATGGA 
CCAGTTTCTG AGGAAAGTGC TAATGGGACC CCCAAGCATT CTTTCAGGAC TAGCTCTACT 
CTGGAAATAG ACTCAGAAGA ATTCACCTCT ACTTCTTCAA GGACCTCACC TCCCAGAGGA 
CGTCAGGATT CACTCAATGA TTACTTAGAT GCTATTGAAC ACAATGGCCA TTCCAGGTCG 
GGTGCAGCTA CTTGTTCGGA AAGGTCCATG GGAGCCTCTC CGAAACTAAG GAGTAGTTTT 
CCCACCGACA CAAGACTCAA TGCAATGCTT CATATCGACT CAGATGAAGA AGACCACGAG 
TTCCAGCAAG ACCTGGGTTA TCCATCCTCC TTGGAGGAGG AAGGAGGGTT GATAATGTTT 
AGCAGGGCGT CAAGAACTGA TGATGGGAGC CTGACATCTC AGATAAAGCC AGAAGACAAC 
ACGGTGGAGA ATGAGGAAGC CTCCACCAAT GAAGCTGCTT CCTTCGAGGA CAAGCCAGAA 
AGTCTTCCAG AGCTTGCGGA GAGCTCCTTC CCCTCAGGCC CAGTCCCTGA TGAAAGCGAG 
AATGCCCCGG AGTGTCAGCC GAGTGCTGAC CAGGGGAGCA CTGAATTGTG CGGCGCCCAG 
GAGGTAGATC AGCCCACAAG TGGGGCAGAC ACGGGCACTT CTGACACGTC AGGAGGAAGC 
CGAAGAGCCG TCAGTGAGAT GGAGTCCCTT GATCAGGGGT CTGAGCCTTC CCAGGTGTCC 
TCGGAAACAG AGCCCAGTGA CCCCGCCAGG ACAGAGAGCG TGAGCGAGGC CAGCACCAGG 
CCTGAGGGAG AGAGTGACCT GGACGGGGCC GACAGCTCCT GCAATGAGAG TGTCACCACG 
CAGCTGTCCT CGGTGGAGAC ACGGTGCTCA TCTCTTGAGA GTGCGCGGTT TCCCGAAACT 
CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT GGAGCCTGTG CGGCAGAGCC CACCAGCAGT 
GGCCCTGCTG AAGGGTCACA GGGTTCCATA TGCACTCCTG GTTCTTTACC TGTAGTGCAG 
GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG GCAGAATCGG CTACTTCACC TGACCAGGAG 
GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG AGCCTGGAGG GAGCTGCTGC TGCTACTGAG 
AGCCCGCCCC AAGAAGAGCG TGATGCGGGG CATGCCCAAG GGGCTTGTGA AGGGGCCACT 
GCCCAGGAGG AGGGCGCGAC CGGAGACTGG GAGGCACGCA TTGACAGCCA TGGCAGGATC 
TTCTACGTGG ATCACGTAAA CAGAACCACG ACTTGGCAGC GGCCCACAGC TCCCCCTGCC 
CCGCAGGTGC TGCAGAGGTC CAACTCCATA CAGCAGATGG AGCAGCTGAA CCGGCGATAC 
CAGAGTATCC GCAGAACCAT GACAAACGAG AGGCCTGAGG AAAACACCAA TGCGATTGAT 
GTGGCAGGGG AGGAAGCCGA CTTTCACCAA GCCAGTGCAG ATTTCCGACG GGAGAACGTC 
CCGCCCCACT CTACCTCCAG ATCCAGGCTC ACGTTGCTGT TACAGTCTCC CCCCGTGAAG 
TTCCTCATCA GCCCAGAGTT CTTCACTGTG CTGCATTCTA ACCCTAGTGC CTACCGCATG 
TTTACAAACA ACACGTGTTT GAAGCACATG ATCACCAAAG TCCGGCGGGA CACCCACCAC 
TTTGAACGCT ACCAGCATAA CCGGGACCTT GTGGGATTCC TCAACATGTT TGCAAACAAA 
CAACTGGAGC TGCCGAGGGG CTGGGAAATG AAACATGATC ATCAGGGCAA GGCATTTTTT 
GTCGACCACA ACTCGCGTAC CACCACCTTC ATCGATCCCC GGCTCCCGCT TCAGAGCAGC 
AGACCCACGA GCGCGCTGGT GCATCGACAG CACCTGACCA GGCAGCGCAG CCACAGCGCA 
GGCGAGGTAG GAGAAGATTC TCGACATGCA GGACCACCAG TTCTTCCCAG GCCATCAAGT 
ACGTTCAATA CAGTTAGTAG GCCACAGTAC CAGGACATGG TTCCAGTGGC TTACAATGAC 
AAGATTGTTG CATTTCTGCG CCAGCCCAAC ATCTTTGAAA TTCTACAGGA GCGTCAACCT 
GATCTTACCA GGAATCACTC ACTCAGGGAG AAGATCCAAT TTATCCGAAC TGAAGGGACC 
CCAGGATTGG TGCGTCTTTC AAGTGATGCC GACCTTGTTA TGTTGCTGAG TTTATTTGAA 
GAAGAGATAA TGTCATATGT GCCTCCACAT GCCTTACTCC ATCCCAGCTA CTGTCAGTCC 
CCACGTGGCT CTCCTGTGTC CTCTCCCCAG AACTCACCAG GTACTCAGCG TGCCAATGCC 
CGTGCTCCAG CCCCTTACAA GCGAGACTTT GAAGCCAAAC TAAGAAACTT TTACAGGAAG 
TTAGAGACTA AAGGATATGG ACAAGGCCCA GGGAAATTAA AGTTAATTAT CAGAAGAGAT 
CACTTGCTCG AAGATGCTTT TAATCAGATT ATGGGCTACT CCAGAAAAGA CCTGCAGAGA 
AATAAGCTGT ATGTCACATT TGTTGGGGAG GAAGGGCTGG ATTACAGTGG ACCTTCCAGA 
GAGTTTTTCT TCCTGGTATC CAGAGAACTC TTTAACCCAT ATTATGGCTT ATTTGAATAT 
TCAGCCAATG ACACATACAC AGTACAAATA AGTCCCATGT CTGCTTTTGT AGACAATCAC 
CATGAATGGT TCCGGTTCAG TGGTAGGATC CTTGGTCTTG CACTAATTCA CCAGTATTTG 
TTGGATGCCT TCTTCACACG GCCCTTTTAT AAGGCTCTTC TCAGAATTCT CTGTGACCTG 
AGTGATCTAG AATACCTTGA TGAAGAATTT CACCAGAGCC TGCAGTGGAT GAAAGACAAT 
GACATCCATG ACATCCTGGA CCTCACGTTC ACTGTGAACG AAGAAGTTTT TGGGCAGATT 
ACCGAACGAG AATTAAAACC AGGGGGTGCT AACATCCCAG TCACGGAGAA GAATAAGAAG 
GAGTACATCG AGAGGATGGT GAAATGGAGG ATTGAGAGGG GTGTTGTGCA GCAAACAGAG 
AGCTTAGTGC GTGGCTTTTA TGAGGTCGTG GATGCCAGGC TGGTATCTGT TTTTGATGCG 
AGAGAACTAG AACTGGTCAT CGCAGGCACA GCTGAAATAG ACCTAAGTGA TTGGAGGAAC 
AACACAGAAT ATAGAGGAGG ATATCATGAC AATCATATTG TAATTCGGTG GTTCTGGGCT 
GCAGTGGAAA GATTCAACAA TGAACAACGA CTAAGGTTGT TACAGTTTGT TACAGGCACA 
TCCAGCATTC CCTATGAAGG ATTTGCTTCT CTCCGAGGGA GTAACGGCCC AAGAAGATTC 
TGTGTGGAGA AATGGGGGAA AATCACCGCT CTTCCCAGTA CAGAGAGCAT TGTCTACTGG 
AGCTATCAGC TTGGAAATTG GAAAGTAACT GAGGAGCTGG TATCACTTTC TTTAATTAAA 
AAAAAATCCA GAACAAGTGA TGGACACTGG GAGGATCGCT TGGTGACACC ACCACTTTTC 
CGTATTGTGC CTGCCTTGGG ACGTCCCCCC TTGGTCTCCC AGTTCCCCAC CACCTGCTGG 
TCCCACGGCA GTCTGCTCCA TCATTTAATC CCTGATACAA GGCCCTCTTC CACCCTTTCC 
CTTTCTTTCC ACTTACTCAA TTTATACTGT CTTATAATAA TATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019794792.1
CDS	2487..6914
Translation	MEAAEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGSQHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFRVEVTSSVHEDASPEAVGTILGVNTVNGDLGSPSDEEDMPGGHHDSTVCSNGPVSEESANGTPKHSFRTSSTLEIDSEEFTSTSSRTSPPRGRQDSLNDYLDAIEHNGHSRSGAATCSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRTDDGSLTSQIKPEDNTVENEEASTNEAASFEDKPESLPELAESSFPSGPVPDESENAPECQPSADQGSTELCGAQEVDQPTSGADTGTSDTSGGSRRAVSEMESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLDGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQGSICTPGSLPVVQVPSGEEEGPGAESATSPDQEEVGEVWQRRGSLEGAAAATESPPQEERDAGHAQGACEGATAQEEGATGDWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTNAIDVAGEEADFHQASADFRRENVPPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPSTESIVYWSYQLGNWKVTEELVSLSLIKKKSRTSDGHWEDRLVTPPLFRIVPALGRPPLVSQFPTTCWSHGSLLHHLIPDTRPSSTLSLSFHLLNLYCLIIIF

Target ORF information:

RefSeq Version	XM_019939233.1
Organism	Tursiops truncatus(common bottlenose dolphin)
Definition	Tursiops truncatus E3 ubiquitin-protein ligase HECW2 (LOC101327442), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019939233.1

ORF Insert Sequence:

ATGGAAGCTG CGGAGATAAA AATCTGTTTC AAATACTACC ACGGCATTAG TGGAGCCCTG 
CGAGCCACGA CCCCCTGCAT CACGGTGAAG AACCCCGCTG TGATGATGGG GGCAGAAGGC 
ATGGAAGGAG GTGCTTCAGG AAGTCAGCAC TCTCGAAAAC TCGTTAGCTT TACGTTGTCT 
GATCTTCGGG CAGTTGGACT AAAGAAAGGG ATGTTCTTCA ATCCTGACCC TTATCTTAAG 
ATGTCGATTC AGCCAGGGAA GAAGAGCAGT TTCCCCACTT GTGCCCACCA TGGGCAGGAG 
AGGAGGTCTA CTATCATCAG TAACACCACA AATCCAATTT GGCACCGAGA GAAATATTCC 
TTTTTCGCAC TCCTTACTGA TGTCTTAGAA ATTGAAATTA AAGACAAATT TGCCAAGAGC 
CGACCTATCA TCAAGCGTTT TCTGGGGAAA CTAACCATTC CAGTCCAGAG GCTTTTGGAG 
CGACAAGCCA TCGGTGACCA GATGCTCAGC TACAACCTCG GCAGAAGGCT CCCAGCTGAC 
CACGTGAGTG GGTACCTCCA GTTCAGAGTG GAGGTCACAT CTTCTGTGCA TGAAGATGCT 
TCTCCAGAAG CTGTTGGCAC AATACTTGGA GTCAATACTG TGAATGGAGA CCTAGGAAGC 
CCTTCTGATG AGGAGGACAT GCCAGGGGGC CATCATGACA GCACAGTTTG TTCCAATGGA 
CCAGTTTCTG AGGAAAGTGC TAATGGGACC CCCAAGCATT CTTTCAGGAC TAGCTCTACT 
CTGGAAATAG ACTCAGAAGA ATTCACCTCT ACTTCTTCAA GGACCTCACC TCCCAGAGGA 
CGTCAGGATT CACTCAATGA TTACTTAGAT GCTATTGAAC ACAATGGCCA TTCCAGGTCG 
GGTGCAGCTA CTTGTTCGGA AAGGTCCATG GGAGCCTCTC CGAAACTAAG GAGTAGTTTT 
CCCACCGACA CAAGACTCAA TGCAATGCTT CATATCGACT CAGATGAAGA AGACCACGAG 
TTCCAGCAAG ACCTGGGTTA TCCATCCTCC TTGGAGGAGG AAGGAGGGTT GATAATGTTT 
AGCAGGGCGT CAAGAACTGA TGATGGGAGC CTGACATCTC AGATAAAGCC AGAAGACAAC 
ACGGTGGAGA ATGAGGAAGC CTCCACCAAT GAAGCTGCTT CCTTCGAGGA CAAGCCAGAA 
AGTCTTCCAG AGCTTGCGGA GAGCTCCTTC CCCTCAGGCC CAGTCCCTGA TGAAAGCGAG 
AATGCCCCGG AGTGTCAGCC GAGTGCTGAC CAGGGGAGCA CTGAATTGTG CGGCGCCCAG 
GAGGTAGATC AGCCCACAAG TGGGGCAGAC ACGGGCACTT CTGACACGTC AGGAGGAAGC 
CGAAGAGCCG TCAGTGAGAT GGAGTCCCTT GATCAGGGGT CTGAGCCTTC CCAGGTGTCC 
TCGGAAACAG AGCCCAGTGA CCCCGCCAGG ACAGAGAGCG TGAGCGAGGC CAGCACCAGG 
CCTGAGGGAG AGAGTGACCT GGACGGGGCC GACAGCTCCT GCAATGAGAG TGTCACCACG 
CAGCTGTCCT CGGTGGAGAC ACGGTGCTCA TCTCTTGAGA GTGCGCGGTT TCCCGAAACT 
CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT GGAGCCTGTG CGGCAGAGCC CACCAGCAGT 
GGCCCTGCTG AAGGGTCACA GGGTTCCATA TGCACTCCTG GTTCTTTACC TGTAGTGCAG 
GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG GCAGAATCGG CTACTTCACC TGACCAGGAG 
GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG AGCCTGGAGG GAGCTGCTGC TGCTACTGAG 
AGCCCGCCCC AAGAAGAGCG TGATGCGGGG CATGCCCAAG GGGCTTGTGA AGGGGCCACT 
GCCCAGGAGG AGGGCGCGAC CGGAGACTGG GAGGCACGCA TTGACAGCCA TGGCAGGATC 
TTCTACGTGG ATCACGTAAA CAGAACCACG ACTTGGCAGC GGCCCACAGC TCCCCCTGCC 
CCGCAGGTGC TGCAGAGGTC CAACTCCATA CAGCAGATGG AGCAGCTGAA CCGGCGATAC 
CAGAGTATCC GCAGAACCAT GACAAACGAG AGGCCTGAGG AAAACACCAA TGCGATTGAT 
GTGGCAGGGG AGGAAGCCGA CTTTCACCAA GCCAGTGCAG ATTTCCGACG GGAGAACGTC 
CCGCCCCACT CTACCTCCAG ATCCAGGCTC ACGTTGCTGT TACAGTCTCC CCCCGTGAAG 
TTCCTCATCA GCCCAGAGTT CTTCACTGTG CTGCATTCTA ACCCTAGTGC CTACCGCATG 
TTTACAAACA ACACGTGTTT GAAGCACATG ATCACCAAAG TCCGGCGGGA CACCCACCAC 
TTTGAACGCT ACCAGCATAA CCGGGACCTT GTGGGATTCC TCAACATGTT TGCAAACAAA 
CAACTGGAGC TGCCGAGGGG CTGGGAAATG AAACATGATC ATCAGGGCAA GGCATTTTTT 
GTCGACCACA ACTCGCGTAC CACCACCTTC ATCGATCCCC GGCTCCCGCT TCAGAGCAGC 
AGACCCACGA GCGCGCTGGT GCATCGACAG CACCTGACCA GGCAGCGCAG CCACAGCGCA 
GGCGAGGTAG GAGAAGATTC TCGACATGCA GGACCACCAG TTCTTCCCAG GCCATCAAGT 
ACGTTCAATA CAGTTAGTAG GCCACAGTAC CAGGACATGG TTCCAGTGGC TTACAATGAC 
AAGATTGTTG CATTTCTGCG CCAGCCCAAC ATCTTTGAAA TTCTACAGGA GCGTCAACCT 
GATCTTACCA GGAATCACTC ACTCAGGGAG AAGATCCAAT TTATCCGAAC TGAAGGGACC 
CCAGGATTGG TGCGTCTTTC AAGTGATGCC GACCTTGTTA TGTTGCTGAG TTTATTTGAA 
GAAGAGATAA TGTCATATGT GCCTCCACAT GCCTTACTCC ATCCCAGCTA CTGTCAGTCC 
CCACGTGGCT CTCCTGTGTC CTCTCCCCAG AACTCACCAG GTACTCAGCG TGCCAATGCC 
CGTGCTCCAG CCCCTTACAA GCGAGACTTT GAAGCCAAAC TAAGAAACTT TTACAGGAAG 
TTAGAGACTA AAGGATATGG ACAAGGCCCA GGGAAATTAA AGTTAATTAT CAGAAGAGAT 
CACTTGCTCG AAGATGCTTT TAATCAGATT ATGGGCTACT CCAGAAAAGA CCTGCAGAGA 
AATAAGCTGT ATGTCACATT TGTTGGGGAG GAAGGGCTGG ATTACAGTGG ACCTTCCAGA 
GAGTTTTTCT TCCTGGTATC CAGAGAACTC TTTAACCCAT ATTATGGCTT ATTTGAATAT 
TCAGCCAATG ACACATACAC AGTACAAATA AGTCCCATGT CTGCTTTTGT AGACAATCAC 
CATGAATGGT TCCGGTTCAG TGGTAGGATC CTTGGTCTTG CACTAATTCA CCAGTATTTG 
TTGGATGCCT TCTTCACACG GCCCTTTTAT AAGGCTCTTC TCAGAATTCT CTGTGACCTG 
AGTGATCTAG AATACCTTGA TGAAGAATTT CACCAGAGCC TGCAGTGGAT GAAAGACAAT 
GACATCCATG ACATCCTGGA CCTCACGTTC ACTGTGAACG AAGAAGTTTT TGGGCAGATT 
ACCGAACGAG AATTAAAACC AGGGGGTGCT AACATCCCAG TCACGGAGAA GAATAAGAAG 
GAGTACATCG AGAGGATGGT GAAATGGAGG ATTGAGAGGG GTGTTGTGCA GCAAACAGAG 
AGCTTAGTGC GTGGCTTTTA TGAGGTCGTG GATGCCAGGC TGGTATCTGT TTTTGATGCG 
AGAGAACTAG AACTGGTCAT CGCAGGCACA GCTGAAATAG ACCTAAGTGA TTGGAGGAAC 
AACACAGAAT ATAGAGGAGG ATATCATGAC AATCATATTG TAATTCGGTG GTTCTGGGCT 
GCAGTGGAAA GATTCAACAA TGAACAACGA CTAAGGTTGT TACAGTTTGT TACAGGCACA 
TCCAGCATTC CCTATGAAGG ATTTGCTTCT CTCCGAGGGA GTAACGGCCC AAGAAGATTC 
TGTGTGGAGA AATGGGGGAA AATCACCGCT CTTCCCAGTA CAGAGAGCAT TGTCTACTGG 
AGCTATCAGC TTGGAAATTG GAAAGTAACT GAGGAGCTGG TATCACTTTC TTTAATTAAA 
AAAAAATCCA GAACAAGTGA TGGACACTGG GAGGATCGCT TGGTGACACC ACCACTTTTC 
CGTATTGTGC CTGCCTTGGG ACGTCCCCCC TTGGTCTCCC AGTTCCCCAC CACCTGCTGG 
TCCCACGGCA GTCTGCTCCA TCATTTAATC CCTGATACAA GGCCCTCTTC CACCCTTTCC 
CTTTCTTTCC ACTTACTCAA TTTATACTGT CTTATAATAA TATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101327442 ( XM_019939234.1 ) cDNA ORF clone, Tursiops truncatus(common bottlenose dolphin) -> XP_019794793.1 Tursiops truncatus E3 ubiquitin-protein ligase HECW2 (LOC101327442), transcript variant X4, mRNA.

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CloneID	OTu45679
Clone ID Related Accession (Same CDS sequence)	XM_019939234.1
Accession Version	XM_019939234.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Tursiops truncatus(common bottlenose dolphin)
Product	E3 ubiquitin-protein ligase HECW2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017843453.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tursiops truncatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGGG CAGAAGGCAT GGAAGGAGGT GCTTCAGGAA GTCAGCACTC TCGAAAACTC 
GTTAGCTTTA CGTTGTCTGA TCTTCGGGCA GTTGGACTAA AGAAAGGGAT GTTCTTCAAT 
CCTGACCCTT ATCTTAAGAT GTCGATTCAG CCAGGGAAGA AGAGCAGTTT CCCCACTTGT 
GCCCACCATG GGCAGGAGAG GAGGTCTACT ATCATCAGTA ACACCACAAA TCCAATTTGG 
CACCGAGAGA AATATTCCTT TTTCGCACTC CTTACTGATG TCTTAGAAAT TGAAATTAAA 
GACAAATTTG CCAAGAGCCG ACCTATCATC AAGCGTTTTC TGGGGAAACT AACCATTCCA 
GTCCAGAGGC TTTTGGAGCG ACAAGCCATC GGTGACCAGA TGCTCAGCTA CAACCTCGGC 
AGAAGGCTCC CAGCTGACCA CGTGAGTGGG TACCTCCAGT TCAGAGTGGA GGTCACATCT 
TCTGTGCATG AAGATGCTTC TCCAGAAGCT GTTGGCACAA TACTTGGAGT CAATACTGTG 
AATGGAGACC TAGGAAGCCC TTCTGATGAG GAGGACATGC CAGGGGGCCA TCATGACAGC 
ACAGTTTGTT CCAATGGACC AGTTTCTGAG GAAAGTGCTA ATGGGACCCC CAAGCATTCT 
TTCAGGACTA GCTCTACTCT GGAAATAGAC TCAGAAGAAT TCACCTCTAC TTCTTCAAGG 
ACCTCACCTC CCAGAGGACG TCAGGATTCA CTCAATGATT ACTTAGATGC TATTGAACAC 
AATGGCCATT CCAGGTCGGG TGCAGCTACT TGTTCGGAAA GGTCCATGGG AGCCTCTCCG 
AAACTAAGGA GTAGTTTTCC CACCGACACA AGACTCAATG CAATGCTTCA TATCGACTCA 
GATGAAGAAG ACCACGAGTT CCAGCAAGAC CTGGGTTATC CATCCTCCTT GGAGGAGGAA 
GGAGGGTTGA TAATGTTTAG CAGGGCGTCA AGAACTGATG ATGGGAGCCT GACATCTCAG 
ATAAAGCCAG AAGACAACAC GGTGGAGAAT GAGGAAGCCT CCACCAATGA AGCTGCTTCC 
TTCGAGGACA AGCCAGAAAG TCTTCCAGAG CTTGCGGAGA GCTCCTTCCC CTCAGGCCCA 
GTCCCTGATG AAAGCGAGAA TGCCCCGGAG TGTCAGCCGA GTGCTGACCA GGGGAGCACT 
GAATTGTGCG GCGCCCAGGA GGTAGATCAG CCCACAAGTG GGGCAGACAC GGGCACTTCT 
GACACGTCAG GAGGAAGCCG AAGAGCCGTC AGTGAGATGG AGTCCCTTGA TCAGGGGTCT 
GAGCCTTCCC AGGTGTCCTC GGAAACAGAG CCCAGTGACC CCGCCAGGAC AGAGAGCGTG 
AGCGAGGCCA GCACCAGGCC TGAGGGAGAG AGTGACCTGG ACGGGGCCGA CAGCTCCTGC 
AATGAGAGTG TCACCACGCA GCTGTCCTCG GTGGAGACAC GGTGCTCATC TCTTGAGAGT 
GCGCGGTTTC CCGAAACTCC AGCCTTTTCT TCTCAGGAGG AGGAAGATGG AGCCTGTGCG 
GCAGAGCCCA CCAGCAGTGG CCCTGCTGAA GGGTCACAGG GTTCCATATG CACTCCTGGT 
TCTTTACCTG TAGTGCAGGT GCCCAGTGGA GAGGAGGAAG GGCCAGGGGC AGAATCGGCT 
ACTTCACCTG ACCAGGAGGA GGTGGGGGAG GTCTGGCAGC GGAGGGGGAG CCTGGAGGGA 
GCTGCTGCTG CTACTGAGAG CCCGCCCCAA GAAGAGCGTG ATGCGGGGCA TGCCCAAGGG 
GCTTGTGAAG GGGCCACTGC CCAGGAGGAG GGCGCGACCG GAGGTTCTCA AGCCAACGGC 
CACCAGCCTT TGCGATCACT GCCTTCAGTG CGCCAGGATG TTAGCCGGTA CCAGAGGGTG 
GACGAGGCTC TCCCACCAAA CTGGGAGGCA CGCATTGACA GCCATGGCAG GATCTTCTAC 
GTGGATCACG TAAACAGAAC CACGACTTGG CAGCGGCCCA CAGCTCCCCC TGCCCCGCAG 
GTGCTGCAGA GGTCCAACTC CATACAGCAG ATGGAGCAGC TGAACCGGCG ATACCAGAGT 
ATCCGCAGAA CCATGACAAA CGAGAGGCCT GAGGAAAACA CCAATGCGAT TGATGTGGCA 
GGGGAGGAAG CCGACTTTCA CCAAGCCAGT GCAGATTTCC GACGGGAGAA CGTCCCGCCC 
CACTCTACCT CCAGATCCAG GCTCACGTTG CTGTTACAGT CTCCCCCCGT GAAGTTCCTC 
ATCAGCCCAG AGTTCTTCAC TGTGCTGCAT TCTAACCCTA GTGCCTACCG CATGTTTACA 
AACAACACGT GTTTGAAGCA CATGATCACC AAAGTCCGGC GGGACACCCA CCACTTTGAA 
CGCTACCAGC ATAACCGGGA CCTTGTGGGA TTCCTCAACA TGTTTGCAAA CAAACAACTG 
GAGCTGCCGA GGGGCTGGGA AATGAAACAT GATCATCAGG GCAAGGCATT TTTTGTCGAC 
CACAACTCGC GTACCACCAC CTTCATCGAT CCCCGGCTCC CGCTTCAGAG CAGCAGACCC 
ACGAGCGCGC TGGTGCATCG ACAGCACCTG ACCAGGCAGC GCAGCCACAG CGCAGGCGAG 
GTAGGAGAAG ATTCTCGACA TGCAGGACCA CCAGTTCTTC CCAGGCCATC AAGTACGTTC 
AATACAGTTA GTAGGCCACA GTACCAGGAC ATGGTTCCAG TGGCTTACAA TGACAAGATT 
GTTGCATTTC TGCGCCAGCC CAACATCTTT GAAATTCTAC AGGAGCGTCA ACCTGATCTT 
ACCAGGAATC ACTCACTCAG GGAGAAGATC CAATTTATCC GAACTGAAGG GACCCCAGGA 
TTGGTGCGTC TTTCAAGTGA TGCCGACCTT GTTATGTTGC TGAGTTTATT TGAAGAAGAG 
ATAATGTCAT ATGTGCCTCC ACATGCCTTA CTCCATCCCA GCTACTGTCA GTCCCCACGT 
GGCTCTCCTG TGTCCTCTCC CCAGAACTCA CCAGGTACTC AGCGTGCCAA TGCCCGTGCT 
CCAGCCCCTT ACAAGCGAGA CTTTGAAGCC AAACTAAGAA ACTTTTACAG GAAGTTAGAG 
ACTAAAGGAT ATGGACAAGG CCCAGGGAAA TTAAAGTTAA TTATCAGAAG AGATCACTTG 
CTCGAAGATG CTTTTAATCA GATTATGGGC TACTCCAGAA AAGACCTGCA GAGAAATAAG 
CTGTATGTCA CATTTGTTGG GGAGGAAGGG CTGGATTACA GTGGACCTTC CAGAGAGTTT 
TTCTTCCTGG TATCCAGAGA ACTCTTTAAC CCATATTATG GCTTATTTGA ATATTCAGCC 
AATGACACAT ACACAGTACA AATAAGTCCC ATGTCTGCTT TTGTAGACAA TCACCATGAA 
TGGTTCCGGT TCAGTGGTAG GATCCTTGGT CTTGCACTAA TTCACCAGTA TTTGTTGGAT 
GCCTTCTTCA CACGGCCCTT TTATAAGGCT CTTCTCAGAA TTCTCTGTGA CCTGAGTGAT 
CTAGAATACC TTGATGAAGA ATTTCACCAG AGCCTGCAGT GGATGAAAGA CAATGACATC 
CATGACATCC TGGACCTCAC GTTCACTGTG AACGAAGAAG TTTTTGGGCA GATTACCGAA 
CGAGAATTAA AACCAGGGGG TGCTAACATC CCAGTCACGG AGAAGAATAA GAAGGAGTAC 
ATCGAGAGGA TGGTGAAATG GAGGATTGAG AGGGGTGTTG TGCAGCAAAC AGAGAGCTTA 
GTGCGTGGCT TTTATGAGGT CGTGGATGCC AGGCTGGTAT CTGTTTTTGA TGCGAGAGAA 
CTAGAACTGG TCATCGCAGG CACAGCTGAA ATAGACCTAA GTGATTGGAG GAACAACACA 
GAATATAGAG GAGGATATCA TGACAATCAT ATTGTAATTC GGTGGTTCTG GGCTGCAGTG 
GAAAGATTCA ACAATGAACA ACGACTAAGG TTGTTACAGT TTGTTACAGG CACATCCAGC 
ATTCCCTATG AAGGATTTGC TTCTCTCCGA GGGAGTAACG GCCCAAGAAG ATTCTGTGTG 
GAGAAATGGG GGAAAATCAC CGCTCTTCCC AGTACAGAGA GCATTGTCTA CTGGAGCTAT 
CAGCTTGGAA ATTGGAAAGT AACTGAGGAG CTGGTATCAC TTTCTTTAAT TAAAAAAAAA 
TCCAGAACAA GTGATGGACA CTGGGAGGAT CGCTTGGTGA CACCACCACT TTTCCGTATT 
GTGCCTGCCT TGGGACGTCC CCCCTTGGTC TCCCAGTTCC CCACCACCTG CTGGTCCCAC 
GGCAGTCTGC TCCATCATTT AATCCCTGAT ACAAGGCCCT CTTCCACCCT TTCCCTTTCT 
TTCCACTTAC TCAATTTATA CTGTCTTATA ATAATATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019794793.1
CDS	1660..6081
Translation	MMGAEGMEGGASGSQHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFRVEVTSSVHEDASPEAVGTILGVNTVNGDLGSPSDEEDMPGGHHDSTVCSNGPVSEESANGTPKHSFRTSSTLEIDSEEFTSTSSRTSPPRGRQDSLNDYLDAIEHNGHSRSGAATCSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRTDDGSLTSQIKPEDNTVENEEASTNEAASFEDKPESLPELAESSFPSGPVPDESENAPECQPSADQGSTELCGAQEVDQPTSGADTGTSDTSGGSRRAVSEMESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLDGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQGSICTPGSLPVVQVPSGEEEGPGAESATSPDQEEVGEVWQRRGSLEGAAAATESPPQEERDAGHAQGACEGATAQEEGATGGSQANGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTNAIDVAGEEADFHQASADFRRENVPPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPSTESIVYWSYQLGNWKVTEELVSLSLIKKKSRTSDGHWEDRLVTPPLFRIVPALGRPPLVSQFPTTCWSHGSLLHHLIPDTRPSSTLSLSFHLLNLYCLIIIF

Target ORF information:

RefSeq Version	XM_019939234.1
Organism	Tursiops truncatus(common bottlenose dolphin)
Definition	Tursiops truncatus E3 ubiquitin-protein ligase HECW2 (LOC101327442), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019939234.1

ORF Insert Sequence:

ATGATGGGGG CAGAAGGCAT GGAAGGAGGT GCTTCAGGAA GTCAGCACTC TCGAAAACTC 
GTTAGCTTTA CGTTGTCTGA TCTTCGGGCA GTTGGACTAA AGAAAGGGAT GTTCTTCAAT 
CCTGACCCTT ATCTTAAGAT GTCGATTCAG CCAGGGAAGA AGAGCAGTTT CCCCACTTGT 
GCCCACCATG GGCAGGAGAG GAGGTCTACT ATCATCAGTA ACACCACAAA TCCAATTTGG 
CACCGAGAGA AATATTCCTT TTTCGCACTC CTTACTGATG TCTTAGAAAT TGAAATTAAA 
GACAAATTTG CCAAGAGCCG ACCTATCATC AAGCGTTTTC TGGGGAAACT AACCATTCCA 
GTCCAGAGGC TTTTGGAGCG ACAAGCCATC GGTGACCAGA TGCTCAGCTA CAACCTCGGC 
AGAAGGCTCC CAGCTGACCA CGTGAGTGGG TACCTCCAGT TCAGAGTGGA GGTCACATCT 
TCTGTGCATG AAGATGCTTC TCCAGAAGCT GTTGGCACAA TACTTGGAGT CAATACTGTG 
AATGGAGACC TAGGAAGCCC TTCTGATGAG GAGGACATGC CAGGGGGCCA TCATGACAGC 
ACAGTTTGTT CCAATGGACC AGTTTCTGAG GAAAGTGCTA ATGGGACCCC CAAGCATTCT 
TTCAGGACTA GCTCTACTCT GGAAATAGAC TCAGAAGAAT TCACCTCTAC TTCTTCAAGG 
ACCTCACCTC CCAGAGGACG TCAGGATTCA CTCAATGATT ACTTAGATGC TATTGAACAC 
AATGGCCATT CCAGGTCGGG TGCAGCTACT TGTTCGGAAA GGTCCATGGG AGCCTCTCCG 
AAACTAAGGA GTAGTTTTCC CACCGACACA AGACTCAATG CAATGCTTCA TATCGACTCA 
GATGAAGAAG ACCACGAGTT CCAGCAAGAC CTGGGTTATC CATCCTCCTT GGAGGAGGAA 
GGAGGGTTGA TAATGTTTAG CAGGGCGTCA AGAACTGATG ATGGGAGCCT GACATCTCAG 
ATAAAGCCAG AAGACAACAC GGTGGAGAAT GAGGAAGCCT CCACCAATGA AGCTGCTTCC 
TTCGAGGACA AGCCAGAAAG TCTTCCAGAG CTTGCGGAGA GCTCCTTCCC CTCAGGCCCA 
GTCCCTGATG AAAGCGAGAA TGCCCCGGAG TGTCAGCCGA GTGCTGACCA GGGGAGCACT 
GAATTGTGCG GCGCCCAGGA GGTAGATCAG CCCACAAGTG GGGCAGACAC GGGCACTTCT 
GACACGTCAG GAGGAAGCCG AAGAGCCGTC AGTGAGATGG AGTCCCTTGA TCAGGGGTCT 
GAGCCTTCCC AGGTGTCCTC GGAAACAGAG CCCAGTGACC CCGCCAGGAC AGAGAGCGTG 
AGCGAGGCCA GCACCAGGCC TGAGGGAGAG AGTGACCTGG ACGGGGCCGA CAGCTCCTGC 
AATGAGAGTG TCACCACGCA GCTGTCCTCG GTGGAGACAC GGTGCTCATC TCTTGAGAGT 
GCGCGGTTTC CCGAAACTCC AGCCTTTTCT TCTCAGGAGG AGGAAGATGG AGCCTGTGCG 
GCAGAGCCCA CCAGCAGTGG CCCTGCTGAA GGGTCACAGG GTTCCATATG CACTCCTGGT 
TCTTTACCTG TAGTGCAGGT GCCCAGTGGA GAGGAGGAAG GGCCAGGGGC AGAATCGGCT 
ACTTCACCTG ACCAGGAGGA GGTGGGGGAG GTCTGGCAGC GGAGGGGGAG CCTGGAGGGA 
GCTGCTGCTG CTACTGAGAG CCCGCCCCAA GAAGAGCGTG ATGCGGGGCA TGCCCAAGGG 
GCTTGTGAAG GGGCCACTGC CCAGGAGGAG GGCGCGACCG GAGGTTCTCA AGCCAACGGC 
CACCAGCCTT TGCGATCACT GCCTTCAGTG CGCCAGGATG TTAGCCGGTA CCAGAGGGTG 
GACGAGGCTC TCCCACCAAA CTGGGAGGCA CGCATTGACA GCCATGGCAG GATCTTCTAC 
GTGGATCACG TAAACAGAAC CACGACTTGG CAGCGGCCCA CAGCTCCCCC TGCCCCGCAG 
GTGCTGCAGA GGTCCAACTC CATACAGCAG ATGGAGCAGC TGAACCGGCG ATACCAGAGT 
ATCCGCAGAA CCATGACAAA CGAGAGGCCT GAGGAAAACA CCAATGCGAT TGATGTGGCA 
GGGGAGGAAG CCGACTTTCA CCAAGCCAGT GCAGATTTCC GACGGGAGAA CGTCCCGCCC 
CACTCTACCT CCAGATCCAG GCTCACGTTG CTGTTACAGT CTCCCCCCGT GAAGTTCCTC 
ATCAGCCCAG AGTTCTTCAC TGTGCTGCAT TCTAACCCTA GTGCCTACCG CATGTTTACA 
AACAACACGT GTTTGAAGCA CATGATCACC AAAGTCCGGC GGGACACCCA CCACTTTGAA 
CGCTACCAGC ATAACCGGGA CCTTGTGGGA TTCCTCAACA TGTTTGCAAA CAAACAACTG 
GAGCTGCCGA GGGGCTGGGA AATGAAACAT GATCATCAGG GCAAGGCATT TTTTGTCGAC 
CACAACTCGC GTACCACCAC CTTCATCGAT CCCCGGCTCC CGCTTCAGAG CAGCAGACCC 
ACGAGCGCGC TGGTGCATCG ACAGCACCTG ACCAGGCAGC GCAGCCACAG CGCAGGCGAG 
GTAGGAGAAG ATTCTCGACA TGCAGGACCA CCAGTTCTTC CCAGGCCATC AAGTACGTTC 
AATACAGTTA GTAGGCCACA GTACCAGGAC ATGGTTCCAG TGGCTTACAA TGACAAGATT 
GTTGCATTTC TGCGCCAGCC CAACATCTTT GAAATTCTAC AGGAGCGTCA ACCTGATCTT 
ACCAGGAATC ACTCACTCAG GGAGAAGATC CAATTTATCC GAACTGAAGG GACCCCAGGA 
TTGGTGCGTC TTTCAAGTGA TGCCGACCTT GTTATGTTGC TGAGTTTATT TGAAGAAGAG 
ATAATGTCAT ATGTGCCTCC ACATGCCTTA CTCCATCCCA GCTACTGTCA GTCCCCACGT 
GGCTCTCCTG TGTCCTCTCC CCAGAACTCA CCAGGTACTC AGCGTGCCAA TGCCCGTGCT 
CCAGCCCCTT ACAAGCGAGA CTTTGAAGCC AAACTAAGAA ACTTTTACAG GAAGTTAGAG 
ACTAAAGGAT ATGGACAAGG CCCAGGGAAA TTAAAGTTAA TTATCAGAAG AGATCACTTG 
CTCGAAGATG CTTTTAATCA GATTATGGGC TACTCCAGAA AAGACCTGCA GAGAAATAAG 
CTGTATGTCA CATTTGTTGG GGAGGAAGGG CTGGATTACA GTGGACCTTC CAGAGAGTTT 
TTCTTCCTGG TATCCAGAGA ACTCTTTAAC CCATATTATG GCTTATTTGA ATATTCAGCC 
AATGACACAT ACACAGTACA AATAAGTCCC ATGTCTGCTT TTGTAGACAA TCACCATGAA 
TGGTTCCGGT TCAGTGGTAG GATCCTTGGT CTTGCACTAA TTCACCAGTA TTTGTTGGAT 
GCCTTCTTCA CACGGCCCTT TTATAAGGCT CTTCTCAGAA TTCTCTGTGA CCTGAGTGAT 
CTAGAATACC TTGATGAAGA ATTTCACCAG AGCCTGCAGT GGATGAAAGA CAATGACATC 
CATGACATCC TGGACCTCAC GTTCACTGTG AACGAAGAAG TTTTTGGGCA GATTACCGAA 
CGAGAATTAA AACCAGGGGG TGCTAACATC CCAGTCACGG AGAAGAATAA GAAGGAGTAC 
ATCGAGAGGA TGGTGAAATG GAGGATTGAG AGGGGTGTTG TGCAGCAAAC AGAGAGCTTA 
GTGCGTGGCT TTTATGAGGT CGTGGATGCC AGGCTGGTAT CTGTTTTTGA TGCGAGAGAA 
CTAGAACTGG TCATCGCAGG CACAGCTGAA ATAGACCTAA GTGATTGGAG GAACAACACA 
GAATATAGAG GAGGATATCA TGACAATCAT ATTGTAATTC GGTGGTTCTG GGCTGCAGTG 
GAAAGATTCA ACAATGAACA ACGACTAAGG TTGTTACAGT TTGTTACAGG CACATCCAGC 
ATTCCCTATG AAGGATTTGC TTCTCTCCGA GGGAGTAACG GCCCAAGAAG ATTCTGTGTG 
GAGAAATGGG GGAAAATCAC CGCTCTTCCC AGTACAGAGA GCATTGTCTA CTGGAGCTAT 
CAGCTTGGAA ATTGGAAAGT AACTGAGGAG CTGGTATCAC TTTCTTTAAT TAAAAAAAAA 
TCCAGAACAA GTGATGGACA CTGGGAGGAT CGCTTGGTGA CACCACCACT TTTCCGTATT 
GTGCCTGCCT TGGGACGTCC CCCCTTGGTC TCCCAGTTCC CCACCACCTG CTGGTCCCAC 
GGCAGTCTGC TCCATCATTT AATCCCTGAT ACAAGGCCCT CTTCCACCCT TTCCCTTTCT 
TTCCACTTAC TCAATTTATA CTGTCTTATA ATAATATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101327442 ( XM_019939235.1 ) cDNA ORF clone, Tursiops truncatus(common bottlenose dolphin) -> XP_019794794.1 Tursiops truncatus E3 ubiquitin-protein ligase HECW2 (LOC101327442), transcript variant X5, mRNA.

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CloneID	OTu45680
Clone ID Related Accession (Same CDS sequence)	XM_019939235.1
Accession Version	XM_019939235.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Tursiops truncatus(common bottlenose dolphin)
Product	E3 ubiquitin-protein ligase HECW2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017843453.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tursiops truncatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCTG CGGAGATAAA AATCTGTTTC AAATACTACC ACGGCATTAG TGGAGCCCTG 
CGAGCCACGA CCCCCTGCAT CACGGTGAAG AACCCCGCTG TGATGATGGG GGCAGAAGGC 
ATGGAAGGAG GTGCTTCAGG AAGTCAGCAC TCTCGAAAAC TCGTTAGCTT TACGTTGTCT 
GATCTTCGGG CAGTTGGACT AAAGAAAGGG ATGTTCTTCA ATCCTGACCC TTATCTTAAG 
ATGTCGATTC AGCCAGGGAA GAAGAGCAGT TTCCCCACTT GTGCCCACCA TGGGCAGGAG 
AGGAGGTCTA CTATCATCAG TAACACCACA AATCCAATTT GGCACCGAGA GAAATATTCC 
TTTTTCGCAC TCCTTACTGA TGTCTTAGAA ATTGAAATTA AAGACAAATT TGCCAAGAGC 
CGACCTATCA TCAAGCGTTT TCTGGGGAAA CTAACCATTC CAGTCCAGAG GCTTTTGGAG 
CGACAAGCCA TCGGTGACCA GATGCTCAGC TACAACCTCG GCAGAAGGCT CCCAGCTGAC 
CACGTGAGTG GGTACCTCCA GTTCAGAGTG GAGGTCACAT CTTCTGTGCA TGAAGATGCT 
TCTCCAGAAG CTGTTGGCAC AATACTTGGA GTCAATACTG TGAATGGAGA CCTAGGAAGC 
CCTTCTGATG AGGAGGACAT GCCAGGGGGC CATCATGACA GCACAGTTTG TTCCAATGGA 
CCAGTTTCTG AGGAAAGTGC TAATGGGACC CCCAAGCATT CTTTCAGGAC TAGCTCTACT 
CTGGAAATAG ACTCAGAAGA ATTCACCTCT ACTTCTTCAA GGACCTCACC TCCCAGAGGA 
CGTCAGGATT CACTCAATGA TTACTTAGAT GCTATTGAAC ACAATGGCCA TTCCAGGTCG 
GGTGCAGCTA CTTGTTCGGA AAGGTCCATG GGAGCCTCTC CGAAACTAAG GAGTAGTTTT 
CCCACCGACA CAAGACTCAA TGCAATGCTT CATATCGACT CAGATGAAGA AGACCACGAG 
TTCCAGCAAG ACCTGGGTTA TCCATCCTCC TTGGAGGAGG AAGGAGGGTT GATAATGTTT 
AGCAGGGCGT CAAGAACTGA TGATGGGAGC CTGACATCTC AGATAAAGCC AGAAGACAAC 
ACGGTGGAGA ATGAGGAAGC CTCCACCAAT GAAGCTGCTT CCTTCGAGGA CAAGCCAGAA 
AGTCTTCCAG AGCTTGCGGA GAGCTCCTTC CCCTCAGGCC CAGTCCCTGA TGAAAGCGAG 
AATGCCCCGG AGTGTCAGCC GAGTGCTGAC CAGGGGAGCA CTGAATTGTG CGGCGCCCAG 
GAGGTAGATC AGCCCACAAG TGGGGCAGAC ACGGGCACTT CTGACACGTC AGGAGGAAGC 
CGAAGAGCCG TCAGTGAGAT GGAGTCCCTT GATCAGGGGT CTGAGCCTTC CCAGGTGTCC 
TCGGAAACAG AGCCCAGTGA CCCCGCCAGG ACAGAGAGCG TGAGCGAGGC CAGCACCAGG 
CCTGAGGGAG AGAGTGACCT GGACGGGGCC GACAGCTCCT GCAATGAGAG TGTCACCACG 
CAGCTGTCCT CGGTGGAGAC ACGGTGCTCA TCTCTTGAGA GTGCGCGGTT TCCCGAAACT 
CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT GGAGCCTGTG CGGCAGAGCC CACCAGCAGT 
GGCCCTGCTG AAGGGTCACA GGGTTCCATA TGCACTCCTG GTTCTTTACC TGTAGTGCAG 
GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG GCAGAATCGG CTACTTCACC TGACCAGGAG 
GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG AGCCTGGAGG GAGCTGCTGC TGCTACTGAG 
AGCCCGCCCC AAGAAGAGCG TGATGCGGGG CATGCCCAAG GGGCTTGTGA AGGGGCCACT 
GCCCAGGAGG AGGGCGCGAC CGGAGGTTCT CAAGCCAACG GCCACCAGCC TTTGCGATCA 
CTGCCTTCAG TGCGCCAGGA TGTTAGCCGG TACCAGAGGG TGGACGAGGC TCTCCCACCA 
AACTGGGAGG CACGCATTGA CAGCCATGGC AGGATCTTCT ACGTGGATCA CGTAAACAGA 
ACCACGACTT GGCAGCGGCC CACAGCTCCC CCTGCCCCGC AGGTGCTGCA GAGGTCCAAC 
TCCATACAGC AGATGGAGCA GCTGAACCGG CGATACCAGA GTATCCGCAG AACCATGACA 
AACGAGAGGC CTGAGGAAAA CACCAATGCG ATTGATGTGG CAGGGGAGGA AGCCGACTTT 
CACCAAGCCA GTGCAGATTT CCGACGGGAG AACGTCCCGC CCCACTCTAC CTCCAGATCC 
AGGCTCACGT TGCTGTTACA GTCTCCCCCC GTGAAGTTCC TCATCAGCCC AGAGTTCTTC 
ACTGTGCTGC ATTCTAACCC TAGTGCCTAC CGCATGTTTA CAAACAACAC GTGTTTGAAG 
CACATGATCA CCAAAGTCCG GCGGGACACC CACCACTTTG AACGCTACCA GCATAACCGG 
GACCTTGTGG GATTCCTCAA CATGTTTGCA AACAAACAAC TGGAGCTGCC GAGGGGCTGG 
GAAATGAAAC ATGATCATCA GGGCAAGGCA TTTTTTGTCG ACCACAACTC GCGTACCACC 
ACCTTCATCG ATCCCCGGCT CCCGCTTCAG AGCAGCAGAC CCACGAGCGC GCTGGTGCAT 
CGACAGCACC TGACCAGGCA GCGCAGCCAC AGCGCAGGCG AGGTAGGAGA AGATTCTCGA 
CATGCAGGAC CACCAGTTCT TCCCAGGCCA TCAAGTACGT TCAATACAGT TAGTAGGCCA 
CAGTACCAGG ACATGGTTCC AGTGGCTTAC AATGACAAGA TTGTTGCATT TCTGCGCCAG 
CCCAACATCT TTGAAATTCT ACAGGAGCGT CAACCTGATC TTACCAGGAA TCACTCACTC 
AGGGAGAAGA TCCAATTTAT CCGAACTGAA GGGACCCCAG GATTGGTGCG TCTTTCAAGT 
GATGCCGACC TTGTTATGTT GCTGAGTTTA TTTGAAGAAG AGATAATGTC ATATGTGCCT 
CCACATGCCT TACTCCATCC CAGCTACTGT CAGTCCCCAC GTGGCTCTCC TGTGTCCTCT 
CCCCAGAACT CACCAGGTAC TCAGCGTGCC AATGCCCGTG CTCCAGCCCC TTACAAGCGA 
GACTTTGAAG CCAAACTAAG AAACTTTTAC AGGAAGTTAG AGACTAAAGG ATATGGACAA 
GGCCCAGGGA AATTAAAGTT AATTATCAGA AGAGATCACT TGCTCGAAGA TGCTTTTAAT 
CAGATTATGG GCTACTCCAG AAAAGACCTG CAGAGAAATA AGCTGTATGT CACATTTGTT 
GGGGAGGAAG GGCTGGATTA CAGTGGACCT TCCAGAGAGT TTTTCTTCCT GGTATCCAGA 
GAACTCTTTA ACCCATATTA TGGCTTATTT GAATATTCAG CCAATGACAC ATACACAGTA 
CAAATAAGTC CCATGTCTGC TTTTGTAGAC AATCACCATG AATGGTTCCG GTTCAGTGGT 
AGGATCCTTG GTCTTGCACT AATTCACCAG TATTTGTTGG ATGCCTTCTT CACACGGCCC 
TTTTATAAGG CTCTTCTCAG AATTCTCTGT GACCTGAGTG ATCTAGAATA CCTTGATGAA 
GAATTTCACC AGAGCCTGCA GTGGATGAAA GACAATGACA TCCATGACAT CCTGGACCTC 
ACGTTCACTG TGAACGAAGA AGTTTTTGGG CAGATTACCG AACGAGAATT AAAACCAGGG 
GGTGCTAACA TCCCAGTCAC GGAGAAGAAT AAGAAGGAGT ACATCGAGAG GATGGTGAAA 
TGGAGGATTG AGAGGGGTGT TGTGCAGCAA ACAGAGAGCT TAGTGCGTGG CTTTTATGAG 
GTCGTGGATG CCAGGCTGGT ATCTGTTTTT GATGCGAGAG AACTAGAACT GGTCATCGCA 
GGCACAGCTG AAATAGACCT AAGTGATTGG AGGAACAACA CAGAATATAG AGGAGGATAT 
CATGACAATC ATATTGTAAT TCGGTGGTTC TGGGCTGCAG TGGAAAGATT CAACAATGAA 
CAACGACTAA GGTTGTTACA GTTTGTTACA GGCACATCCA GCATTCCCTA TGAAGGATTT 
GCTTCTCTCC GAGGGAGTAA CGGCCCAAGA AGATTCTGTG TGGAGAAATG GGGGAAAATC 
ACCGCTCTTC CCAGAGCTCA CACTTGTTTT AACCGTCTGG ATCTCCCTCC GTACCCATCC 
TTCTCCATGC TTTATGAAAA ACTCTTGACA GCAGTTGAAG AGACCAGTAC CTTTGGACTT 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019794794.1
CDS	2486..6811
Translation	MEAAEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGSQHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFRVEVTSSVHEDASPEAVGTILGVNTVNGDLGSPSDEEDMPGGHHDSTVCSNGPVSEESANGTPKHSFRTSSTLEIDSEEFTSTSSRTSPPRGRQDSLNDYLDAIEHNGHSRSGAATCSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRTDDGSLTSQIKPEDNTVENEEASTNEAASFEDKPESLPELAESSFPSGPVPDESENAPECQPSADQGSTELCGAQEVDQPTSGADTGTSDTSGGSRRAVSEMESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLDGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQGSICTPGSLPVVQVPSGEEEGPGAESATSPDQEEVGEVWQRRGSLEGAAAATESPPQEERDAGHAQGACEGATAQEEGATGGSQANGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTNAIDVAGEEADFHQASADFRRENVPPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_019939235.1
Organism	Tursiops truncatus(common bottlenose dolphin)
Definition	Tursiops truncatus E3 ubiquitin-protein ligase HECW2 (LOC101327442), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019939235.1

ORF Insert Sequence:

ATGGAAGCTG CGGAGATAAA AATCTGTTTC AAATACTACC ACGGCATTAG TGGAGCCCTG 
CGAGCCACGA CCCCCTGCAT CACGGTGAAG AACCCCGCTG TGATGATGGG GGCAGAAGGC 
ATGGAAGGAG GTGCTTCAGG AAGTCAGCAC TCTCGAAAAC TCGTTAGCTT TACGTTGTCT 
GATCTTCGGG CAGTTGGACT AAAGAAAGGG ATGTTCTTCA ATCCTGACCC TTATCTTAAG 
ATGTCGATTC AGCCAGGGAA GAAGAGCAGT TTCCCCACTT GTGCCCACCA TGGGCAGGAG 
AGGAGGTCTA CTATCATCAG TAACACCACA AATCCAATTT GGCACCGAGA GAAATATTCC 
TTTTTCGCAC TCCTTACTGA TGTCTTAGAA ATTGAAATTA AAGACAAATT TGCCAAGAGC 
CGACCTATCA TCAAGCGTTT TCTGGGGAAA CTAACCATTC CAGTCCAGAG GCTTTTGGAG 
CGACAAGCCA TCGGTGACCA GATGCTCAGC TACAACCTCG GCAGAAGGCT CCCAGCTGAC 
CACGTGAGTG GGTACCTCCA GTTCAGAGTG GAGGTCACAT CTTCTGTGCA TGAAGATGCT 
TCTCCAGAAG CTGTTGGCAC AATACTTGGA GTCAATACTG TGAATGGAGA CCTAGGAAGC 
CCTTCTGATG AGGAGGACAT GCCAGGGGGC CATCATGACA GCACAGTTTG TTCCAATGGA 
CCAGTTTCTG AGGAAAGTGC TAATGGGACC CCCAAGCATT CTTTCAGGAC TAGCTCTACT 
CTGGAAATAG ACTCAGAAGA ATTCACCTCT ACTTCTTCAA GGACCTCACC TCCCAGAGGA 
CGTCAGGATT CACTCAATGA TTACTTAGAT GCTATTGAAC ACAATGGCCA TTCCAGGTCG 
GGTGCAGCTA CTTGTTCGGA AAGGTCCATG GGAGCCTCTC CGAAACTAAG GAGTAGTTTT 
CCCACCGACA CAAGACTCAA TGCAATGCTT CATATCGACT CAGATGAAGA AGACCACGAG 
TTCCAGCAAG ACCTGGGTTA TCCATCCTCC TTGGAGGAGG AAGGAGGGTT GATAATGTTT 
AGCAGGGCGT CAAGAACTGA TGATGGGAGC CTGACATCTC AGATAAAGCC AGAAGACAAC 
ACGGTGGAGA ATGAGGAAGC CTCCACCAAT GAAGCTGCTT CCTTCGAGGA CAAGCCAGAA 
AGTCTTCCAG AGCTTGCGGA GAGCTCCTTC CCCTCAGGCC CAGTCCCTGA TGAAAGCGAG 
AATGCCCCGG AGTGTCAGCC GAGTGCTGAC CAGGGGAGCA CTGAATTGTG CGGCGCCCAG 
GAGGTAGATC AGCCCACAAG TGGGGCAGAC ACGGGCACTT CTGACACGTC AGGAGGAAGC 
CGAAGAGCCG TCAGTGAGAT GGAGTCCCTT GATCAGGGGT CTGAGCCTTC CCAGGTGTCC 
TCGGAAACAG AGCCCAGTGA CCCCGCCAGG ACAGAGAGCG TGAGCGAGGC CAGCACCAGG 
CCTGAGGGAG AGAGTGACCT GGACGGGGCC GACAGCTCCT GCAATGAGAG TGTCACCACG 
CAGCTGTCCT CGGTGGAGAC ACGGTGCTCA TCTCTTGAGA GTGCGCGGTT TCCCGAAACT 
CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT GGAGCCTGTG CGGCAGAGCC CACCAGCAGT 
GGCCCTGCTG AAGGGTCACA GGGTTCCATA TGCACTCCTG GTTCTTTACC TGTAGTGCAG 
GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG GCAGAATCGG CTACTTCACC TGACCAGGAG 
GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG AGCCTGGAGG GAGCTGCTGC TGCTACTGAG 
AGCCCGCCCC AAGAAGAGCG TGATGCGGGG CATGCCCAAG GGGCTTGTGA AGGGGCCACT 
GCCCAGGAGG AGGGCGCGAC CGGAGGTTCT CAAGCCAACG GCCACCAGCC TTTGCGATCA 
CTGCCTTCAG TGCGCCAGGA TGTTAGCCGG TACCAGAGGG TGGACGAGGC TCTCCCACCA 
AACTGGGAGG CACGCATTGA CAGCCATGGC AGGATCTTCT ACGTGGATCA CGTAAACAGA 
ACCACGACTT GGCAGCGGCC CACAGCTCCC CCTGCCCCGC AGGTGCTGCA GAGGTCCAAC 
TCCATACAGC AGATGGAGCA GCTGAACCGG CGATACCAGA GTATCCGCAG AACCATGACA 
AACGAGAGGC CTGAGGAAAA CACCAATGCG ATTGATGTGG CAGGGGAGGA AGCCGACTTT 
CACCAAGCCA GTGCAGATTT CCGACGGGAG AACGTCCCGC CCCACTCTAC CTCCAGATCC 
AGGCTCACGT TGCTGTTACA GTCTCCCCCC GTGAAGTTCC TCATCAGCCC AGAGTTCTTC 
ACTGTGCTGC ATTCTAACCC TAGTGCCTAC CGCATGTTTA CAAACAACAC GTGTTTGAAG 
CACATGATCA CCAAAGTCCG GCGGGACACC CACCACTTTG AACGCTACCA GCATAACCGG 
GACCTTGTGG GATTCCTCAA CATGTTTGCA AACAAACAAC TGGAGCTGCC GAGGGGCTGG 
GAAATGAAAC ATGATCATCA GGGCAAGGCA TTTTTTGTCG ACCACAACTC GCGTACCACC 
ACCTTCATCG ATCCCCGGCT CCCGCTTCAG AGCAGCAGAC CCACGAGCGC GCTGGTGCAT 
CGACAGCACC TGACCAGGCA GCGCAGCCAC AGCGCAGGCG AGGTAGGAGA AGATTCTCGA 
CATGCAGGAC CACCAGTTCT TCCCAGGCCA TCAAGTACGT TCAATACAGT TAGTAGGCCA 
CAGTACCAGG ACATGGTTCC AGTGGCTTAC AATGACAAGA TTGTTGCATT TCTGCGCCAG 
CCCAACATCT TTGAAATTCT ACAGGAGCGT CAACCTGATC TTACCAGGAA TCACTCACTC 
AGGGAGAAGA TCCAATTTAT CCGAACTGAA GGGACCCCAG GATTGGTGCG TCTTTCAAGT 
GATGCCGACC TTGTTATGTT GCTGAGTTTA TTTGAAGAAG AGATAATGTC ATATGTGCCT 
CCACATGCCT TACTCCATCC CAGCTACTGT CAGTCCCCAC GTGGCTCTCC TGTGTCCTCT 
CCCCAGAACT CACCAGGTAC TCAGCGTGCC AATGCCCGTG CTCCAGCCCC TTACAAGCGA 
GACTTTGAAG CCAAACTAAG AAACTTTTAC AGGAAGTTAG AGACTAAAGG ATATGGACAA 
GGCCCAGGGA AATTAAAGTT AATTATCAGA AGAGATCACT TGCTCGAAGA TGCTTTTAAT 
CAGATTATGG GCTACTCCAG AAAAGACCTG CAGAGAAATA AGCTGTATGT CACATTTGTT 
GGGGAGGAAG GGCTGGATTA CAGTGGACCT TCCAGAGAGT TTTTCTTCCT GGTATCCAGA 
GAACTCTTTA ACCCATATTA TGGCTTATTT GAATATTCAG CCAATGACAC ATACACAGTA 
CAAATAAGTC CCATGTCTGC TTTTGTAGAC AATCACCATG AATGGTTCCG GTTCAGTGGT 
AGGATCCTTG GTCTTGCACT AATTCACCAG TATTTGTTGG ATGCCTTCTT CACACGGCCC 
TTTTATAAGG CTCTTCTCAG AATTCTCTGT GACCTGAGTG ATCTAGAATA CCTTGATGAA 
GAATTTCACC AGAGCCTGCA GTGGATGAAA GACAATGACA TCCATGACAT CCTGGACCTC 
ACGTTCACTG TGAACGAAGA AGTTTTTGGG CAGATTACCG AACGAGAATT AAAACCAGGG 
GGTGCTAACA TCCCAGTCAC GGAGAAGAAT AAGAAGGAGT ACATCGAGAG GATGGTGAAA 
TGGAGGATTG AGAGGGGTGT TGTGCAGCAA ACAGAGAGCT TAGTGCGTGG CTTTTATGAG 
GTCGTGGATG CCAGGCTGGT ATCTGTTTTT GATGCGAGAG AACTAGAACT GGTCATCGCA 
GGCACAGCTG AAATAGACCT AAGTGATTGG AGGAACAACA CAGAATATAG AGGAGGATAT 
CATGACAATC ATATTGTAAT TCGGTGGTTC TGGGCTGCAG TGGAAAGATT CAACAATGAA 
CAACGACTAA GGTTGTTACA GTTTGTTACA GGCACATCCA GCATTCCCTA TGAAGGATTT 
GCTTCTCTCC GAGGGAGTAA CGGCCCAAGA AGATTCTGTG TGGAGAAATG GGGGAAAATC 
ACCGCTCTTC CCAGAGCTCA CACTTGTTTT AACCGTCTGG ATCTCCCTCC GTACCCATCC 
TTCTCCATGC TTTATGAAAA ACTCTTGACA GCAGTTGAAG AGACCAGTAC CTTTGGACTT 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101327442 ( XM_019939236.1 ) cDNA ORF clone, Tursiops truncatus(common bottlenose dolphin) -> XP_019794795.1 Tursiops truncatus E3 ubiquitin-protein ligase HECW2 (LOC101327442), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OTu45681
Clone ID Related Accession (Same CDS sequence)	XM_019939236.1
Accession Version	XM_019939236.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4263bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Tursiops truncatus(common bottlenose dolphin)
Product	E3 ubiquitin-protein ligase HECW2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017843453.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tursiops truncatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCTG CGGAGATAAA AATCTGTTTC AAATACTACC ACGGCATTAG TGGAGCCCTG 
CGAGCCACGA CCCCCTGCAT CACGGTGAAG AACCCCGCTG TGATGATGGG GGCAGAAGGC 
ATGGAAGGAG GTGCTTCAGG AAGTCAGCAC TCTCGAAAAC TCGTTAGCTT TACGTTGTCT 
GATCTTCGGG CAGTTGGACT AAAGAAAGGG ATGTTCTTCA ATCCTGACCC TTATCTTAAG 
ATGTCGATTC AGCCAGGGAA GAAGAGCAGT TTCCCCACTT GTGCCCACCA TGGGCAGGAG 
AGGAGGTCTA CTATCATCAG TAACACCACA AATCCAATTT GGCACCGAGA GAAATATTCC 
TTTTTCGCAC TCCTTACTGA TGTCTTAGAA ATTGAAATTA AAGACAAATT TGCCAAGAGC 
CGACCTATCA TCAAGCGTTT TCTGGGGAAA CTAACCATTC CAGTCCAGAG GCTTTTGGAG 
CGACAAGCCA TCGGTGACCA GATGCTCAGC TACAACCTCG GCAGAAGGCT CCCAGCTGAC 
CACGTGAGTG GGTACCTCCA GTTCAGAGTG GAGGTCACAT CTTCTGTGCA TGAAGATGCT 
TCTCCAGAAG CTGTTGGCAC AATACTTGGA GTCAATACTG TGAATGGAGA CCTAGGAAGC 
CCTTCTGATG AGGAGGACAT GCCAGGGGGC CATCATGACA GCACAGTTTG TTCCAATGGA 
CCAGTTTCTG AGGAAAGTGC TAATGGGACC CCCAAGCATT CTTTCAGGAC TAGCTCTACT 
CTGGAAATAG ACTCAGAAGA ATTCACCTCT ACTTCTTCAA GGACCTCACC TCCCAGAGGA 
CGTCAGGATT CACTCAATGA TTACTTAGAT GCTATTGAAC ACAATGGCCA TTCCAGGTCG 
GGTGCAGCTA CTTGTTCGGA AAGGTCCATG GGAGCCTCTC CGAAACTAAG GAGTAGTTTT 
CCCACCGACA CAAGACTCAA TGCAATGCTT CATATCGACT CAGATGAAGA AGACCACGAG 
TTCCAGCAAG ACCTGGGTTA TCCATCCTCC TTGGAGGAGG AAGGAGGGTT GATAATGTTT 
AGCAGGGCGT CAAGAACTGA TGATGGGAGC CTGACATCTC AGATAAAGCC AGAAGACAAC 
ACGGTGGAGA ATGAGGAAGC CTCCACCAAT GAAGCTGCTT CCTTCGAGGA CAAGCCAGAA 
AGTCTTCCAG AGCTTGCGGA GAGCTCCTTC CCCTCAGGCC CAGTCCCTGA TGAAAGCGAG 
AATGCCCCGG AGTGTCAGCC GAGTGCTGAC CAGGGGAGCA CTGAATTGTG CGGCGCCCAG 
GAGGTAGATC AGCCCACAAG TGGGGCAGAC ACGGGCACTT CTGACACGTC AGGAGGAAGC 
CGAAGAGCCG TCAGTGAGAT GGAGTCCCTT GATCAGGGGT CTGAGCCTTC CCAGGTGTCC 
TCGGAAACAG AGCCCAGTGA CCCCGCCAGG ACAGAGAGCG TGAGCGAGGC CAGCACCAGG 
CCTGAGGGAG AGAGTGACCT GGACGGGGCC GACAGCTCCT GCAATGAGAG TGTCACCACG 
CAGCTGTCCT CGGTGGAGAC ACGGTGCTCA TCTCTTGAGA GTGCGCGGTT TCCCGAAACT 
CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT GGAGCCTGTG CGGCAGAGCC CACCAGCAGT 
GGCCCTGCTG AAGGGTCACA GGGTTCCATA TGCACTCCTG GTTCTTTACC TGTAGTGCAG 
GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG GCAGAATCGG CTACTTCACC TGACCAGGAG 
GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG AGCCTGGAGG GAGCTGCTGC TGCTACTGAG 
AGCCCGCCCC AAGAAGAGCG TGATGCGGGG CATGCCCAAG GGGCTTGTGA AGGGGCCACT 
GCCCAGGAGG AGGGCGCGAC CGGAGGTTCT CAAGCCAACG GCCACCAGCC TTTGCGATCA 
CTGCCTTCAG TGCGCCAGGA TGTTAGCCGG TACCAGAGGG TGGACGAGGC TCTCCCACCA 
AACTGGGAGG CACGCATTGA CAGCCATGGC AGGATCTTCT ACGTGGATCA CGTAAACAGA 
ACCACGACTT GGCAGCGGCC CACAGCTCCC CCTGCCCCGC AGGTGCTGCA GAGGTCCAAC 
TCCATACAGC AGATGGAGCA GCTGAACCGG CGATACCAGA GTATCCGCAG AACCATGACA 
AACGAGAGGC CTGAGGAAAA CACCAATGCG ATTGATGTGG CAGGGGAGGA AGCCGACTTT 
CACCAAGCCA GTGCAGATTT CCGACGGGAG AACGTCCCGC CCCACTCTAC CTCCAGATCC 
AGGCTCACGT TGCTGTTACA GTCTCCCCCC GTGAAGTTCC TCATCAGCCC AGAGTTCTTC 
ACTGTGCTGC ATTCTAACCC TAGTGCCTAC CGCATGTTTA CAAACAACAC GTGTTTGAAG 
CACATGATCA CCAAAGTCCG GCGGGACACC CACCACTTTG AACGCTACCA GCATAACCGG 
GACCTTGTGG GATTCCTCAA CATGTTTGCA AACAAACAAC TGGAGCTGCC GAGGGGCTGG 
GAAATGAAAC ATGATCATCA GGGCAAGGCA TTTTTTGTCG ACCACAACTC GCGTACCACC 
ACCTTCATCG ATCCCCGGCT CCCGCTTCAG AGCAGCAGAC CCACGAGCGC GCTGGTGCAT 
CGACAGCACC TGACCAGGCA GCGCAGCCAC AGCGCAGGCG AGGTAGGAGA AGATTCTCGA 
CATGCAGGAC CACCAGTTCT TCCCAGGCCA TCAAGTACGT TCAATACAGT TAGTAGGCCA 
CAGTACCAGG ACATGGTTCC AGTGGCTTAC AATGACAAGA TTGTTGCATT TCTGCGCCAG 
CCCAACATCT TTGAAATTCT ACAGGAGCGT CAACCTGATC TTACCAGGAA TCACTCACTC 
AGGGAGAAGA TCCAATTTAT CCGAACTGAA GGGACCCCAG GATTGGTGCG TCTTTCAAGT 
GATGCCGACC TTGTTATGTT GCTGAGTTTA TTTGAAGAAG AGATAATGTC ATATGTGCCT 
CCACATGCCT TACTCCATCC CAGCTACTGT CAGTCCCCAC GTGGCTCTCC TGTGTCCTCT 
CCCCAGAACT CACCAGGTAC TCAGCGTGCC AATGCCCGTG CTCCAGCCCC TTACAAGCGA 
GACTTTGAAG CCAAACTAAG AAACTTTTAC AGGAAGTTAG AGACTAAAGG ATATGGACAA 
GGCCCAGGGA AATTAAAGTT AATTATCAGA AGAGATCACT TGCTCGAAGA TGCTTTTAAT 
CAGATTATGG GCTACTCCAG AAAAGACCTG CAGAGAAATA AGCTGTATGT CACATTTGTT 
GGGGAGGAAG GGCTGGATTA CAGTGGACCT TCCAGAGAGT TTTTCTTCCT GGTATCCAGA 
GAACTCTTTA ACCCATATTA TGGCTTATTT GAATATTCAG CCAATGACAC ATACACAGTA 
CAAATAAGTC CCATGTCTGC TTTTGTAGAC AATCACCATG AATGGTTCCG GTTCAGTGGT 
AGGATCCTTG GTCTTGCACT AATTCACCAG TATTTGTTGG ATGCCTTCTT CACACGGCCC 
TTTTATAAGG CTCTTCTCAG AATTCTCTGT GACCTGAGTG ATCTAGAATA CCTTGATGAA 
GAATTTCACC AGAGCCTGCA GTGGATGAAA GACAATGACA TCCATGACAT CCTGGACCTC 
ACGTTCACTG TGAACGAAGA AGTTTTTGGG CAGATTACCG AACGAGAATT AAAACCAGGG 
GGTGCTAACA TCCCAGTCAC GGAGAAGAAT AAGAAGGAGT ACATCGAGAG GATGGTGAAA 
TGGAGGATTG AGAGGGGTGT TGTGCAGCAA ACAGAGAGCT TAGTGCGTGG CTTTTATGAG 
GTCGTGGATG CCAGGCTGGT ATCTGTTTTT GATGCGAGAG AACTAGAACT GGTCATCGCA 
GGCACAGCTG AAATAGACCT AAGTGATTGG AGGAACAACA CAGAATATAG AGGAGGATAT 
CATGACAATC ATATTGTAAT TCGGTGGTTC TGGGCTGCAG TGGAAAGATT CAACAATGAA 
CAACGACTAA GGTTGTTACA GTTTGTTACA GGCACATCCA GCATTCCCTA TGAAGGATTT 
GCTTCTCTCC GAGGGAGTAA CGGCCCAAGA AGATTCTGTG TGGAGAAATG GGGGAAAATC 
ACCGCTCTTC CCAGAGAGCA TTGTCTACTG GAGCTATCAG CTTGGAAATT GGAAAGTAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019794795.1
CDS	2487..6749
Translation	MEAAEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGSQHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFRVEVTSSVHEDASPEAVGTILGVNTVNGDLGSPSDEEDMPGGHHDSTVCSNGPVSEESANGTPKHSFRTSSTLEIDSEEFTSTSSRTSPPRGRQDSLNDYLDAIEHNGHSRSGAATCSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRTDDGSLTSQIKPEDNTVENEEASTNEAASFEDKPESLPELAESSFPSGPVPDESENAPECQPSADQGSTELCGAQEVDQPTSGADTGTSDTSGGSRRAVSEMESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLDGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQGSICTPGSLPVVQVPSGEEEGPGAESATSPDQEEVGEVWQRRGSLEGAAAATESPPQEERDAGHAQGACEGATAQEEGATGGSQANGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTNAIDVAGEEADFHQASADFRRENVPPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPREHCLLELSAWKLESN

Target ORF information:

RefSeq Version	XM_019939236.1
Organism	Tursiops truncatus(common bottlenose dolphin)
Definition	Tursiops truncatus E3 ubiquitin-protein ligase HECW2 (LOC101327442), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019939236.1

ORF Insert Sequence:

ATGGAAGCTG CGGAGATAAA AATCTGTTTC AAATACTACC ACGGCATTAG TGGAGCCCTG 
CGAGCCACGA CCCCCTGCAT CACGGTGAAG AACCCCGCTG TGATGATGGG GGCAGAAGGC 
ATGGAAGGAG GTGCTTCAGG AAGTCAGCAC TCTCGAAAAC TCGTTAGCTT TACGTTGTCT 
GATCTTCGGG CAGTTGGACT AAAGAAAGGG ATGTTCTTCA ATCCTGACCC TTATCTTAAG 
ATGTCGATTC AGCCAGGGAA GAAGAGCAGT TTCCCCACTT GTGCCCACCA TGGGCAGGAG 
AGGAGGTCTA CTATCATCAG TAACACCACA AATCCAATTT GGCACCGAGA GAAATATTCC 
TTTTTCGCAC TCCTTACTGA TGTCTTAGAA ATTGAAATTA AAGACAAATT TGCCAAGAGC 
CGACCTATCA TCAAGCGTTT TCTGGGGAAA CTAACCATTC CAGTCCAGAG GCTTTTGGAG 
CGACAAGCCA TCGGTGACCA GATGCTCAGC TACAACCTCG GCAGAAGGCT CCCAGCTGAC 
CACGTGAGTG GGTACCTCCA GTTCAGAGTG GAGGTCACAT CTTCTGTGCA TGAAGATGCT 
TCTCCAGAAG CTGTTGGCAC AATACTTGGA GTCAATACTG TGAATGGAGA CCTAGGAAGC 
CCTTCTGATG AGGAGGACAT GCCAGGGGGC CATCATGACA GCACAGTTTG TTCCAATGGA 
CCAGTTTCTG AGGAAAGTGC TAATGGGACC CCCAAGCATT CTTTCAGGAC TAGCTCTACT 
CTGGAAATAG ACTCAGAAGA ATTCACCTCT ACTTCTTCAA GGACCTCACC TCCCAGAGGA 
CGTCAGGATT CACTCAATGA TTACTTAGAT GCTATTGAAC ACAATGGCCA TTCCAGGTCG 
GGTGCAGCTA CTTGTTCGGA AAGGTCCATG GGAGCCTCTC CGAAACTAAG GAGTAGTTTT 
CCCACCGACA CAAGACTCAA TGCAATGCTT CATATCGACT CAGATGAAGA AGACCACGAG 
TTCCAGCAAG ACCTGGGTTA TCCATCCTCC TTGGAGGAGG AAGGAGGGTT GATAATGTTT 
AGCAGGGCGT CAAGAACTGA TGATGGGAGC CTGACATCTC AGATAAAGCC AGAAGACAAC 
ACGGTGGAGA ATGAGGAAGC CTCCACCAAT GAAGCTGCTT CCTTCGAGGA CAAGCCAGAA 
AGTCTTCCAG AGCTTGCGGA GAGCTCCTTC CCCTCAGGCC CAGTCCCTGA TGAAAGCGAG 
AATGCCCCGG AGTGTCAGCC GAGTGCTGAC CAGGGGAGCA CTGAATTGTG CGGCGCCCAG 
GAGGTAGATC AGCCCACAAG TGGGGCAGAC ACGGGCACTT CTGACACGTC AGGAGGAAGC 
CGAAGAGCCG TCAGTGAGAT GGAGTCCCTT GATCAGGGGT CTGAGCCTTC CCAGGTGTCC 
TCGGAAACAG AGCCCAGTGA CCCCGCCAGG ACAGAGAGCG TGAGCGAGGC CAGCACCAGG 
CCTGAGGGAG AGAGTGACCT GGACGGGGCC GACAGCTCCT GCAATGAGAG TGTCACCACG 
CAGCTGTCCT CGGTGGAGAC ACGGTGCTCA TCTCTTGAGA GTGCGCGGTT TCCCGAAACT 
CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT GGAGCCTGTG CGGCAGAGCC CACCAGCAGT 
GGCCCTGCTG AAGGGTCACA GGGTTCCATA TGCACTCCTG GTTCTTTACC TGTAGTGCAG 
GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG GCAGAATCGG CTACTTCACC TGACCAGGAG 
GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG AGCCTGGAGG GAGCTGCTGC TGCTACTGAG 
AGCCCGCCCC AAGAAGAGCG TGATGCGGGG CATGCCCAAG GGGCTTGTGA AGGGGCCACT 
GCCCAGGAGG AGGGCGCGAC CGGAGGTTCT CAAGCCAACG GCCACCAGCC TTTGCGATCA 
CTGCCTTCAG TGCGCCAGGA TGTTAGCCGG TACCAGAGGG TGGACGAGGC TCTCCCACCA 
AACTGGGAGG CACGCATTGA CAGCCATGGC AGGATCTTCT ACGTGGATCA CGTAAACAGA 
ACCACGACTT GGCAGCGGCC CACAGCTCCC CCTGCCCCGC AGGTGCTGCA GAGGTCCAAC 
TCCATACAGC AGATGGAGCA GCTGAACCGG CGATACCAGA GTATCCGCAG AACCATGACA 
AACGAGAGGC CTGAGGAAAA CACCAATGCG ATTGATGTGG CAGGGGAGGA AGCCGACTTT 
CACCAAGCCA GTGCAGATTT CCGACGGGAG AACGTCCCGC CCCACTCTAC CTCCAGATCC 
AGGCTCACGT TGCTGTTACA GTCTCCCCCC GTGAAGTTCC TCATCAGCCC AGAGTTCTTC 
ACTGTGCTGC ATTCTAACCC TAGTGCCTAC CGCATGTTTA CAAACAACAC GTGTTTGAAG 
CACATGATCA CCAAAGTCCG GCGGGACACC CACCACTTTG AACGCTACCA GCATAACCGG 
GACCTTGTGG GATTCCTCAA CATGTTTGCA AACAAACAAC TGGAGCTGCC GAGGGGCTGG 
GAAATGAAAC ATGATCATCA GGGCAAGGCA TTTTTTGTCG ACCACAACTC GCGTACCACC 
ACCTTCATCG ATCCCCGGCT CCCGCTTCAG AGCAGCAGAC CCACGAGCGC GCTGGTGCAT 
CGACAGCACC TGACCAGGCA GCGCAGCCAC AGCGCAGGCG AGGTAGGAGA AGATTCTCGA 
CATGCAGGAC CACCAGTTCT TCCCAGGCCA TCAAGTACGT TCAATACAGT TAGTAGGCCA 
CAGTACCAGG ACATGGTTCC AGTGGCTTAC AATGACAAGA TTGTTGCATT TCTGCGCCAG 
CCCAACATCT TTGAAATTCT ACAGGAGCGT CAACCTGATC TTACCAGGAA TCACTCACTC 
AGGGAGAAGA TCCAATTTAT CCGAACTGAA GGGACCCCAG GATTGGTGCG TCTTTCAAGT 
GATGCCGACC TTGTTATGTT GCTGAGTTTA TTTGAAGAAG AGATAATGTC ATATGTGCCT 
CCACATGCCT TACTCCATCC CAGCTACTGT CAGTCCCCAC GTGGCTCTCC TGTGTCCTCT 
CCCCAGAACT CACCAGGTAC TCAGCGTGCC AATGCCCGTG CTCCAGCCCC TTACAAGCGA 
GACTTTGAAG CCAAACTAAG AAACTTTTAC AGGAAGTTAG AGACTAAAGG ATATGGACAA 
GGCCCAGGGA AATTAAAGTT AATTATCAGA AGAGATCACT TGCTCGAAGA TGCTTTTAAT 
CAGATTATGG GCTACTCCAG AAAAGACCTG CAGAGAAATA AGCTGTATGT CACATTTGTT 
GGGGAGGAAG GGCTGGATTA CAGTGGACCT TCCAGAGAGT TTTTCTTCCT GGTATCCAGA 
GAACTCTTTA ACCCATATTA TGGCTTATTT GAATATTCAG CCAATGACAC ATACACAGTA 
CAAATAAGTC CCATGTCTGC TTTTGTAGAC AATCACCATG AATGGTTCCG GTTCAGTGGT 
AGGATCCTTG GTCTTGCACT AATTCACCAG TATTTGTTGG ATGCCTTCTT CACACGGCCC 
TTTTATAAGG CTCTTCTCAG AATTCTCTGT GACCTGAGTG ATCTAGAATA CCTTGATGAA 
GAATTTCACC AGAGCCTGCA GTGGATGAAA GACAATGACA TCCATGACAT CCTGGACCTC 
ACGTTCACTG TGAACGAAGA AGTTTTTGGG CAGATTACCG AACGAGAATT AAAACCAGGG 
GGTGCTAACA TCCCAGTCAC GGAGAAGAAT AAGAAGGAGT ACATCGAGAG GATGGTGAAA 
TGGAGGATTG AGAGGGGTGT TGTGCAGCAA ACAGAGAGCT TAGTGCGTGG CTTTTATGAG 
GTCGTGGATG CCAGGCTGGT ATCTGTTTTT GATGCGAGAG AACTAGAACT GGTCATCGCA 
GGCACAGCTG AAATAGACCT AAGTGATTGG AGGAACAACA CAGAATATAG AGGAGGATAT 
CATGACAATC ATATTGTAAT TCGGTGGTTC TGGGCTGCAG TGGAAAGATT CAACAATGAA 
CAACGACTAA GGTTGTTACA GTTTGTTACA GGCACATCCA GCATTCCCTA TGAAGGATTT 
GCTTCTCTCC GAGGGAGTAA CGGCCCAAGA AGATTCTGTG TGGAGAAATG GGGGAAAATC 
ACCGCTCTTC CCAGAGAGCA TTGTCTACTG GAGCTATCAG CTTGGAAATT GGAAAGTAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101327442 ( XM_019939237.1 ) cDNA ORF clone, Tursiops truncatus(common bottlenose dolphin) -> XP_019794796.1 Tursiops truncatus E3 ubiquitin-protein ligase HECW2 (LOC101327442), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OTu45682
Clone ID Related Accession (Same CDS sequence)	XM_019939237.1
Accession Version	XM_019939237.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4224bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Tursiops truncatus(common bottlenose dolphin)
Product	E3 ubiquitin-protein ligase HECW2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017843453.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tursiops truncatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCTG CGGAGATAAA AATCTGTTTC AAATACTACC ACGGCATTAG TGGAGCCCTG 
CGAGCCACGA CCCCCTGCAT CACGGTGAAG AACCCCGCTG TGATGATGGG GGCAGAAGGC 
ATGGAAGGAG GTGCTTCAGG AAGTCAGCAC TCTCGAAAAC TCGTTAGCTT TACGTTGTCT 
GATCTTCGGG CAGTTGGACT AAAGAAAGGG ATGTTCTTCA ATCCTGACCC TTATCTTAAG 
ATGTCGATTC AGCCAGGGAA GAAGAGCAGT TTCCCCACTT GTGCCCACCA TGGGCAGGAG 
AGGAGGTCTA CTATCATCAG TAACACCACA AATCCAATTT GGCACCGAGA GAAATATTCC 
TTTTTCGCAC TCCTTACTGA TGTCTTAGAA ATTGAAATTA AAGACAAATT TGCCAAGAGC 
CGACCTATCA TCAAGCGTTT TCTGGGGAAA CTAACCATTC CAGTCCAGAG GCTTTTGGAG 
CGACAAGCCA TCGGTGACCA GATGCTCAGC TACAACCTCG GCAGAAGGCT CCCAGCTGAC 
CACGTGAGTG GGTACCTCCA GTTCAGAGTG GAGGTCACAT CTTCTGTGCA TGAAGATGCT 
TCTCCAGAAG CTGTTGGCAC AATACTTGGA GTCAATACTG TGAATGGAGA CCTAGGAAGC 
CCTTCTGATG AGGAGGACAT GCCAGGGGGC CATCATGACA GCACAGTTTG TTCCAATGGA 
CCAGTTTCTG AGGAAAGTGC TAATGGGACC CCCAAGCATT CTTTCAGGAC TAGCTCTACT 
CTGGAAATAG ACTCAGAAGA ATTCACCTCT ACTTCTTCAA GGACCTCACC TCCCAGAGGA 
CGTCAGGATT CACTCAATGA TTACTTAGAT GCTATTGAAC ACAATGGCCA TTCCAGGTCG 
GGTGCAGCTA CTTGTTCGGA AAGGTCCATG GGAGCCTCTC CGAAACTAAG GAGTAGTTTT 
CCCACCGACA CAAGACTCAA TGCAATGCTT CATATCGACT CAGATGAAGA AGACCACGAG 
TTCCAGCAAG ACCTGGGTTA TCCATCCTCC TTGGAGGAGG AAGGAGGGTT GATAATGTTT 
AGCAGGGCGT CAAGAACTGA TGATGGGAGC CTGACATCTC AGATAAAGCC AGAAGACAAC 
ACGGTGGAGA ATGAGGAAGC CTCCACCAAT GAAGCTGCTT CCTTCGAGGA CAAGCCAGAA 
AGTCTTCCAG AGCTTGCGGA GAGCTCCTTC CCCTCAGGCC CAGTCCCTGA TGAAAGCGAG 
AATGCCCCGG AGTGTCAGCC GAGTGCTGAC CAGGGGAGCA CTGAATTGTG CGGCGCCCAG 
GAGGTAGATC AGCCCACAAG TGGGGCAGAC ACGGGCACTT CTGACACGTC AGGAGGAAGC 
CGAAGAGCCG TCAGTGAGAT GGAGTCCCTT GATCAGGGGT CTGAGCCTTC CCAGGTGTCC 
TCGGAAACAG AGCCCAGTGA CCCCGCCAGG ACAGAGAGCG TGAGCGAGGC CAGCACCAGG 
CCTGAGGGAG AGAGTGACCT GGACGGGGCC GACAGCTCCT GCAATGAGAG TGTCACCACG 
CAGCTGTCCT CGGTGGAGAC ACGGTGCTCA TCTCTTGAGA GTGCGCGGTT TCCCGAAACT 
CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT GGAGCCTGTG CGGCAGAGCC CACCAGCAGT 
GGCCCTGCTG AAGGGTCACA GGGTTCCATA TGCACTCCTG GTTCTTTACC TGTAGTGCAG 
GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG GCAGAATCGG CTACTTCACC TGACCAGGAG 
GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG AGCCTGGAGG GAGCTGCTGC TGCTACTGAG 
AGCCCGCCCC AAGAAGAGCG TGATGCGGGG CATGCCCAAG GGGCTTGTGA AGGGGCCACT 
GCCCAGGAGG AGGGCGCGAC CGGAGGTTCT CAAGCCAACG GCCACCAGCC TTTGCGATCA 
CTGCCTTCAG TGCGCCAGGA TGTTAGCCGG TACCAGAGGG TGGACGAGGC TCTCCCACCA 
AACTGGGAGG CACGCATTGA CAGCCATGGC AGGATCTTCT ACGTGGATCA CGTAAACAGA 
ACCACGACTT GGCAGCGGCC CACAGCTCCC CCTGCCCCGC AGGTGCTGCA GAGGTCCAAC 
TCCATACAGC AGATGGAGCA GCTGAACCGG CGATACCAGA GTATCCGCAG AACCATGACA 
AACGAGAGGC CTGAGGAAAA CACCAATGCG ATTGATGTGG CAGGGGAGGA AGCCGACTTT 
CACCAAGCCA GTGCAGATTT CCGACGGGAG AACGTCCCGC CCCACTCTAC CTCCAGATCC 
AGGCTCACGT TGCTGTTACA GTCTCCCCCC GTGAAGTTCC TCATCAGCCC AGAGTTCTTC 
ACTGTGCTGC ATTCTAACCC TAGTGCCTAC CGCATGTTTA CAAACAACAC GTGTTTGAAG 
CACATGATCA CCAAAGTCCG GCGGGACACC CACCACTTTG AACGCTACCA GCATAACCGG 
GACCTTGTGG GATTCCTCAA CATGTTTGCA AACAAACAAC TGGAGCTGCC GAGGGGCTGG 
GAAATGAAAC ATGATCATCA GGGCAAGGCA TTTTTTGTCG ACCACAACTC GCGTACCACC 
ACCTTCATCG ATCCCCGGCT CCCGCTTCAG AGCAGCAGAC CCACGAGCGC GCTGGTGCAT 
CGACAGCACC TGACCAGGCA GCGCAGCCAC AGCGCAGGCG AGGTAGGAGA AGATTCTCGA 
CATGCAGGAC CACCAGTTCT TCCCAGGCCA TCAAGTACGT TCAATACAGT TAGTAGGCCA 
CAGTACCAGG ACATGGTTCC AGTGGCTTAC AATGACAAGA TTGTTGCATT TCTGCGCCAG 
CCCAACATCT TTGAAATTCT ACAGGAGCGT CAACCTGATC TTACCAGGAA TCACTCACTC 
AGGGAGAAGA TCCAATTTAT CCGAACTGAA GGGACCCCAG GATTGGTGCG TCTTTCAAGT 
GATGCCGACC TTGTTATGTT GCTGAGTTTA TTTGAAGAAG AGATAATGTC ATATGTGCCT 
CCACATGCCT TACTCCATCC CAGCTACTGT CAGTCCCCAC GTGGCTCTCC TGTGTCCTCT 
CCCCAGAACT CACCAGGTAC TCAGCGTGCC AATGCCCGTG CTCCAGCCCC TTACAAGCGA 
GACTTTGAAG CCAAACTAAG AAACTTTTAC AGGAAGTTAG AGACTAAAGG ATATGGACAA 
GGCCCAGGGA AATTAAAGTT AATTATCAGA AGAGATCACT TGCTCGAAGA TGCTTTTAAT 
CAGATTATGG GCTACTCCAG AAAAGACCTG CAGAGAAATA AGCTGTATGT CACATTTGTT 
GGGGAGGAAG GGCTGGATTA CAGTGGACCT TCCAGAGAGT TTTTCTTCCT GGTATCCAGA 
GAACTCTTTA ACCCATATTA TGGCTTATTT GAATATTCAG CCAATGACAC ATACACAGTA 
CAAATAAGTC CCATGTCTGC TTTTGTAGAC AATCACCATG AATGGTTCCG GTTCAGTGGT 
AGGATCCTTG GTCTTGCACT AATTCACCAG TATTTGTTGG ATGCCTTCTT CACACGGCCC 
TTTTATAAGG CTCTTCTCAG AATTCTCTGT GACCTGAGTG ATCTAGAATA CCTTGATGAA 
GAATTTCACC AGAGCCTGCA GTGGATGAAA GACAATGACA TCCATGACAT CCTGGACCTC 
ACGTTCACTG TGAACGAAGA AGTTTTTGGG CAGATTACCG AACGAGAATT AAAACCAGGG 
GGTGCTAACA TCCCAGTCAC GGAGAAGAAT AAGAAGGAGT ACATCGAGAG GATGGTGAAA 
TGGAGGATTG AGAGGGGTGT TGTGCAGCAA ACAGAGAGCT TAGTGCGTGG CTTTTATGAG 
GTCGTGGATG CCAGGCTGGT ATCTGTTTTT GATGCGAGAG AACTAGAACT GGTCATCGCA 
GGCACAGCTG AAATAGACCT AAGTGATTGG AGGAACAACA CAGAATATAG AGGAGGATAT 
CATGACAATC ATATTGTAAT TCGGTGGTTC TGGGCTGCAG TGGAAAGATT CAACAATGAA 
CAACGACTAA GGTTGTTACA GTTTGTTACA GGCACATCCA GCATTCCCTA TGAAGGATTT 
GCTTCTCTCC GAGGGAGTAA CGGCCCAAGA AGATTCTGTG TGGAGAAATG GGGGAAAATC 
ACCGCTCTTC CCAGGTGCTG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019794796.1
CDS	2487..6710
Translation	MEAAEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGSQHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFRVEVTSSVHEDASPEAVGTILGVNTVNGDLGSPSDEEDMPGGHHDSTVCSNGPVSEESANGTPKHSFRTSSTLEIDSEEFTSTSSRTSPPRGRQDSLNDYLDAIEHNGHSRSGAATCSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRTDDGSLTSQIKPEDNTVENEEASTNEAASFEDKPESLPELAESSFPSGPVPDESENAPECQPSADQGSTELCGAQEVDQPTSGADTGTSDTSGGSRRAVSEMESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLDGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQGSICTPGSLPVVQVPSGEEEGPGAESATSPDQEEVGEVWQRRGSLEGAAAATESPPQEERDAGHAQGACEGATAQEEGATGGSQANGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTNAIDVAGEEADFHQASADFRRENVPPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRCC

Target ORF information:

RefSeq Version	XM_019939237.1
Organism	Tursiops truncatus(common bottlenose dolphin)
Definition	Tursiops truncatus E3 ubiquitin-protein ligase HECW2 (LOC101327442), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019939237.1

ORF Insert Sequence:

ATGGAAGCTG CGGAGATAAA AATCTGTTTC AAATACTACC ACGGCATTAG TGGAGCCCTG 
CGAGCCACGA CCCCCTGCAT CACGGTGAAG AACCCCGCTG TGATGATGGG GGCAGAAGGC 
ATGGAAGGAG GTGCTTCAGG AAGTCAGCAC TCTCGAAAAC TCGTTAGCTT TACGTTGTCT 
GATCTTCGGG CAGTTGGACT AAAGAAAGGG ATGTTCTTCA ATCCTGACCC TTATCTTAAG 
ATGTCGATTC AGCCAGGGAA GAAGAGCAGT TTCCCCACTT GTGCCCACCA TGGGCAGGAG 
AGGAGGTCTA CTATCATCAG TAACACCACA AATCCAATTT GGCACCGAGA GAAATATTCC 
TTTTTCGCAC TCCTTACTGA TGTCTTAGAA ATTGAAATTA AAGACAAATT TGCCAAGAGC 
CGACCTATCA TCAAGCGTTT TCTGGGGAAA CTAACCATTC CAGTCCAGAG GCTTTTGGAG 
CGACAAGCCA TCGGTGACCA GATGCTCAGC TACAACCTCG GCAGAAGGCT CCCAGCTGAC 
CACGTGAGTG GGTACCTCCA GTTCAGAGTG GAGGTCACAT CTTCTGTGCA TGAAGATGCT 
TCTCCAGAAG CTGTTGGCAC AATACTTGGA GTCAATACTG TGAATGGAGA CCTAGGAAGC 
CCTTCTGATG AGGAGGACAT GCCAGGGGGC CATCATGACA GCACAGTTTG TTCCAATGGA 
CCAGTTTCTG AGGAAAGTGC TAATGGGACC CCCAAGCATT CTTTCAGGAC TAGCTCTACT 
CTGGAAATAG ACTCAGAAGA ATTCACCTCT ACTTCTTCAA GGACCTCACC TCCCAGAGGA 
CGTCAGGATT CACTCAATGA TTACTTAGAT GCTATTGAAC ACAATGGCCA TTCCAGGTCG 
GGTGCAGCTA CTTGTTCGGA AAGGTCCATG GGAGCCTCTC CGAAACTAAG GAGTAGTTTT 
CCCACCGACA CAAGACTCAA TGCAATGCTT CATATCGACT CAGATGAAGA AGACCACGAG 
TTCCAGCAAG ACCTGGGTTA TCCATCCTCC TTGGAGGAGG AAGGAGGGTT GATAATGTTT 
AGCAGGGCGT CAAGAACTGA TGATGGGAGC CTGACATCTC AGATAAAGCC AGAAGACAAC 
ACGGTGGAGA ATGAGGAAGC CTCCACCAAT GAAGCTGCTT CCTTCGAGGA CAAGCCAGAA 
AGTCTTCCAG AGCTTGCGGA GAGCTCCTTC CCCTCAGGCC CAGTCCCTGA TGAAAGCGAG 
AATGCCCCGG AGTGTCAGCC GAGTGCTGAC CAGGGGAGCA CTGAATTGTG CGGCGCCCAG 
GAGGTAGATC AGCCCACAAG TGGGGCAGAC ACGGGCACTT CTGACACGTC AGGAGGAAGC 
CGAAGAGCCG TCAGTGAGAT GGAGTCCCTT GATCAGGGGT CTGAGCCTTC CCAGGTGTCC 
TCGGAAACAG AGCCCAGTGA CCCCGCCAGG ACAGAGAGCG TGAGCGAGGC CAGCACCAGG 
CCTGAGGGAG AGAGTGACCT GGACGGGGCC GACAGCTCCT GCAATGAGAG TGTCACCACG 
CAGCTGTCCT CGGTGGAGAC ACGGTGCTCA TCTCTTGAGA GTGCGCGGTT TCCCGAAACT 
CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT GGAGCCTGTG CGGCAGAGCC CACCAGCAGT 
GGCCCTGCTG AAGGGTCACA GGGTTCCATA TGCACTCCTG GTTCTTTACC TGTAGTGCAG 
GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG GCAGAATCGG CTACTTCACC TGACCAGGAG 
GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG AGCCTGGAGG GAGCTGCTGC TGCTACTGAG 
AGCCCGCCCC AAGAAGAGCG TGATGCGGGG CATGCCCAAG GGGCTTGTGA AGGGGCCACT 
GCCCAGGAGG AGGGCGCGAC CGGAGGTTCT CAAGCCAACG GCCACCAGCC TTTGCGATCA 
CTGCCTTCAG TGCGCCAGGA TGTTAGCCGG TACCAGAGGG TGGACGAGGC TCTCCCACCA 
AACTGGGAGG CACGCATTGA CAGCCATGGC AGGATCTTCT ACGTGGATCA CGTAAACAGA 
ACCACGACTT GGCAGCGGCC CACAGCTCCC CCTGCCCCGC AGGTGCTGCA GAGGTCCAAC 
TCCATACAGC AGATGGAGCA GCTGAACCGG CGATACCAGA GTATCCGCAG AACCATGACA 
AACGAGAGGC CTGAGGAAAA CACCAATGCG ATTGATGTGG CAGGGGAGGA AGCCGACTTT 
CACCAAGCCA GTGCAGATTT CCGACGGGAG AACGTCCCGC CCCACTCTAC CTCCAGATCC 
AGGCTCACGT TGCTGTTACA GTCTCCCCCC GTGAAGTTCC TCATCAGCCC AGAGTTCTTC 
ACTGTGCTGC ATTCTAACCC TAGTGCCTAC CGCATGTTTA CAAACAACAC GTGTTTGAAG 
CACATGATCA CCAAAGTCCG GCGGGACACC CACCACTTTG AACGCTACCA GCATAACCGG 
GACCTTGTGG GATTCCTCAA CATGTTTGCA AACAAACAAC TGGAGCTGCC GAGGGGCTGG 
GAAATGAAAC ATGATCATCA GGGCAAGGCA TTTTTTGTCG ACCACAACTC GCGTACCACC 
ACCTTCATCG ATCCCCGGCT CCCGCTTCAG AGCAGCAGAC CCACGAGCGC GCTGGTGCAT 
CGACAGCACC TGACCAGGCA GCGCAGCCAC AGCGCAGGCG AGGTAGGAGA AGATTCTCGA 
CATGCAGGAC CACCAGTTCT TCCCAGGCCA TCAAGTACGT TCAATACAGT TAGTAGGCCA 
CAGTACCAGG ACATGGTTCC AGTGGCTTAC AATGACAAGA TTGTTGCATT TCTGCGCCAG 
CCCAACATCT TTGAAATTCT ACAGGAGCGT CAACCTGATC TTACCAGGAA TCACTCACTC 
AGGGAGAAGA TCCAATTTAT CCGAACTGAA GGGACCCCAG GATTGGTGCG TCTTTCAAGT 
GATGCCGACC TTGTTATGTT GCTGAGTTTA TTTGAAGAAG AGATAATGTC ATATGTGCCT 
CCACATGCCT TACTCCATCC CAGCTACTGT CAGTCCCCAC GTGGCTCTCC TGTGTCCTCT 
CCCCAGAACT CACCAGGTAC TCAGCGTGCC AATGCCCGTG CTCCAGCCCC TTACAAGCGA 
GACTTTGAAG CCAAACTAAG AAACTTTTAC AGGAAGTTAG AGACTAAAGG ATATGGACAA 
GGCCCAGGGA AATTAAAGTT AATTATCAGA AGAGATCACT TGCTCGAAGA TGCTTTTAAT 
CAGATTATGG GCTACTCCAG AAAAGACCTG CAGAGAAATA AGCTGTATGT CACATTTGTT 
GGGGAGGAAG GGCTGGATTA CAGTGGACCT TCCAGAGAGT TTTTCTTCCT GGTATCCAGA 
GAACTCTTTA ACCCATATTA TGGCTTATTT GAATATTCAG CCAATGACAC ATACACAGTA 
CAAATAAGTC CCATGTCTGC TTTTGTAGAC AATCACCATG AATGGTTCCG GTTCAGTGGT 
AGGATCCTTG GTCTTGCACT AATTCACCAG TATTTGTTGG ATGCCTTCTT CACACGGCCC 
TTTTATAAGG CTCTTCTCAG AATTCTCTGT GACCTGAGTG ATCTAGAATA CCTTGATGAA 
GAATTTCACC AGAGCCTGCA GTGGATGAAA GACAATGACA TCCATGACAT CCTGGACCTC 
ACGTTCACTG TGAACGAAGA AGTTTTTGGG CAGATTACCG AACGAGAATT AAAACCAGGG 
GGTGCTAACA TCCCAGTCAC GGAGAAGAAT AAGAAGGAGT ACATCGAGAG GATGGTGAAA 
TGGAGGATTG AGAGGGGTGT TGTGCAGCAA ACAGAGAGCT TAGTGCGTGG CTTTTATGAG 
GTCGTGGATG CCAGGCTGGT ATCTGTTTTT GATGCGAGAG AACTAGAACT GGTCATCGCA 
GGCACAGCTG AAATAGACCT AAGTGATTGG AGGAACAACA CAGAATATAG AGGAGGATAT 
CATGACAATC ATATTGTAAT TCGGTGGTTC TGGGCTGCAG TGGAAAGATT CAACAATGAA 
CAACGACTAA GGTTGTTACA GTTTGTTACA GGCACATCCA GCATTCCCTA TGAAGGATTT 
GCTTCTCTCC GAGGGAGTAA CGGCCCAAGA AGATTCTGTG TGGAGAAATG GGGGAAAATC 
ACCGCTCTTC CCAGGTGCTG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101327442 ( XM_019939231.1 ) cDNA ORF clone, Tursiops truncatus(common bottlenose dolphin) -> XP_019794790.1 Tursiops truncatus E3 ubiquitin-protein ligase HECW2 (LOC101327442), transcript variant X1, mRNA.

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CloneID	OTu45676
Clone ID Related Accession (Same CDS sequence)	XM_019939231.1
Accession Version	XM_019939231.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Tursiops truncatus(common bottlenose dolphin)
Product	E3 ubiquitin-protein ligase HECW2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017843453.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tursiops truncatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCTG CGGAGATAAA AATCTGTTTC AAATACTACC ACGGCATTAG TGGAGCCCTG 
CGAGCCACGA CCCCCTGCAT CACGGTGAAG AACCCCGCTG TGATGATGGG GGCAGAAGGC 
ATGGAAGGAG GTGCTTCAGG AAGTCAGCAC TCTCGAAAAC TCGTTAGCTT TACGTTGTCT 
GATCTTCGGG CAGTTGGACT AAAGAAAGGG ATGTTCTTCA ATCCTGACCC TTATCTTAAG 
ATGTCGATTC AGCCAGGGAA GAAGAGCAGT TTCCCCACTT GTGCCCACCA TGGGCAGGAG 
AGGAGGTCTA CTATCATCAG TAACACCACA AATCCAATTT GGCACCGAGA GAAATATTCC 
TTTTTCGCAC TCCTTACTGA TGTCTTAGAA ATTGAAATTA AAGACAAATT TGCCAAGAGC 
CGACCTATCA TCAAGCGTTT TCTGGGGAAA CTAACCATTC CAGTCCAGAG GCTTTTGGAG 
CGACAAGCCA TCGGTGACCA GATGCTCAGC TACAACCTCG GCAGAAGGCT CCCAGCTGAC 
CACGTGAGTG GGTACCTCCA GTTCAGAGTG GAGGTCACAT CTTCTGTGCA TGAAGATGCT 
TCTCCAGAAG CTGTTGGCAC AATACTTGGA GTCAATACTG TGAATGGAGA CCTAGGAAGC 
CCTTCTGATG AGGAGGACAT GCCAGGGGGC CATCATGACA GCACAGTTTG TTCCAATGGA 
CCAGTTTCTG AGGAAAGTGC TAATGGGACC CCCAAGCATT CTTTCAGGAC TAGCTCTACT 
CTGGAAATAG ACTCAGAAGA ATTCACCTCT ACTTCTTCAA GGACCTCACC TCCCAGAGGA 
CGTCAGGATT CACTCAATGA TTACTTAGAT GCTATTGAAC ACAATGGCCA TTCCAGGTCG 
GGTGCAGCTA CTTGTTCGGA AAGGTCCATG GGAGCCTCTC CGAAACTAAG GAGTAGTTTT 
CCCACCGACA CAAGACTCAA TGCAATGCTT CATATCGACT CAGATGAAGA AGACCACGAG 
TTCCAGCAAG ACCTGGGTTA TCCATCCTCC TTGGAGGAGG AAGGAGGGTT GATAATGTTT 
AGCAGGGCGT CAAGAACTGA TGATGGGAGC CTGACATCTC AGATAAAGCC AGAAGACAAC 
ACGGTGGAGA ATGAGGAAGC CTCCACCAAT GAAGCTGCTT CCTTCGAGGA CAAGCCAGAA 
AGTCTTCCAG AGCTTGCGGA GAGCTCCTTC CCCTCAGGCC CAGTCCCTGA TGAAAGCGAG 
AATGCCCCGG AGTGTCAGCC GAGTGCTGAC CAGGGGAGCA CTGAATTGTG CGGCGCCCAG 
GAGGTAGATC AGCCCACAAG TGGGGCAGAC ACGGGCACTT CTGACACGTC AGGAGGAAGC 
CGAAGAGCCG TCAGTGAGAT GGAGTCCCTT GATCAGGGGT CTGAGCCTTC CCAGGTGTCC 
TCGGAAACAG AGCCCAGTGA CCCCGCCAGG ACAGAGAGCG TGAGCGAGGC CAGCACCAGG 
CCTGAGGGAG AGAGTGACCT GGACGGGGCC GACAGCTCCT GCAATGAGAG TGTCACCACG 
CAGCTGTCCT CGGTGGAGAC ACGGTGCTCA TCTCTTGAGA GTGCGCGGTT TCCCGAAACT 
CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT GGAGCCTGTG CGGCAGAGCC CACCAGCAGT 
GGCCCTGCTG AAGGGTCACA GGGTTCCATA TGCACTCCTG GTTCTTTACC TGTAGTGCAG 
GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG GCAGAATCGG CTACTTCACC TGACCAGGAG 
GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG AGCCTGGAGG GAGCTGCTGC TGCTACTGAG 
AGCCCGCCCC AAGAAGAGCG TGATGCGGGG CATGCCCAAG GGGCTTGTGA AGGGGCCACT 
GCCCAGGAGG AGGGCGCGAC CGGAGGTTCT CAAGCCAACG GCCACCAGCC TTTGCGATCA 
CTGCCTTCAG TGCGCCAGGA TGTTAGCCGG TACCAGAGGG TGGACGAGGC TCTCCCACCA 
AACTGGGAGG CACGCATTGA CAGCCATGGC AGGATCTTCT ACGTGGATCA CGTAAACAGA 
ACCACGACTT GGCAGCGGCC CACAGCTCCC CCTGCCCCGC AGGTGCTGCA GAGGTCCAAC 
TCCATACAGC AGATGGAGCA GCTGAACCGG CGATACCAGA GTATCCGCAG AACCATGACA 
AACGAGAGGC CTGAGGAAAA CACCAATGCG ATTGATGTGG CAGGGGAGGA AGCCGACTTT 
CACCAAGCCA GTGCAGATTT CCGACGGGAG AACGTCCCGC CCCACTCTAC CTCCAGATCC 
AGGCTCACGT TGCTGTTACA GTCTCCCCCC GTGAAGTTCC TCATCAGCCC AGAGTTCTTC 
ACTGTGCTGC ATTCTAACCC TAGTGCCTAC CGCATGTTTA CAAACAACAC GTGTTTGAAG 
CACATGATCA CCAAAGTCCG GCGGGACACC CACCACTTTG AACGCTACCA GCATAACCGG 
GACCTTGTGG GATTCCTCAA CATGTTTGCA AACAAACAAC TGGAGCTGCC GAGGGGCTGG 
GAAATGAAAC ATGATCATCA GGGCAAGGCA TTTTTTGTCG ACCACAACTC GCGTACCACC 
ACCTTCATCG ATCCCCGGCT CCCGCTTCAG AGCAGCAGAC CCACGAGCGC GCTGGTGCAT 
CGACAGCACC TGACCAGGCA GCGCAGCCAC AGCGCAGGCG AGGTAGGAGA AGATTCTCGA 
CATGCAGGAC CACCAGTTCT TCCCAGGCCA TCAAGTACGT TCAATACAGT TAGTAGGCCA 
CAGTACCAGG ACATGGTTCC AGTGGCTTAC AATGACAAGA TTGTTGCATT TCTGCGCCAG 
CCCAACATCT TTGAAATTCT ACAGGAGCGT CAACCTGATC TTACCAGGAA TCACTCACTC 
AGGGAGAAGA TCCAATTTAT CCGAACTGAA GGGACCCCAG GATTGGTGCG TCTTTCAAGT 
GATGCCGACC TTGTTATGTT GCTGAGTTTA TTTGAAGAAG AGATAATGTC ATATGTGCCT 
CCACATGCCT TACTCCATCC CAGCTACTGT CAGTCCCCAC GTGGCTCTCC TGTGTCCTCT 
CCCCAGAACT CACCAGGTAC TCAGCGTGCC AATGCCCGTG CTCCAGCCCC TTACAAGCGA 
GACTTTGAAG CCAAACTAAG AAACTTTTAC AGGAAGTTAG AGACTAAAGG ATATGGACAA 
GGCCCAGGGA AATTAAAGTT AATTATCAGA AGAGATCACT TGCTCGAAGA TGCTTTTAAT 
CAGATTATGG GCTACTCCAG AAAAGACCTG CAGAGAAATA AGCTGTATGT CACATTTGTT 
GGGGAGGAAG GGCTGGATTA CAGTGGACCT TCCAGAGAGT TTTTCTTCCT GGTATCCAGA 
GAACTCTTTA ACCCATATTA TGGCTTATTT GAATATTCAG CCAATGACAC ATACACAGTA 
CAAATAAGTC CCATGTCTGC TTTTGTAGAC AATCACCATG AATGGTTCCG GTTCAGTGGT 
AGGATCCTTG GTCTTGCACT AATTCACCAG TATTTGTTGG ATGCCTTCTT CACACGGCCC 
TTTTATAAGG CTCTTCTCAG AATTCTCTGT GACCTGAGTG ATCTAGAATA CCTTGATGAA 
GAATTTCACC AGAGCCTGCA GTGGATGAAA GACAATGACA TCCATGACAT CCTGGACCTC 
ACGTTCACTG TGAACGAAGA AGTTTTTGGG CAGATTACCG AACGAGAATT AAAACCAGGG 
GGTGCTAACA TCCCAGTCAC GGAGAAGAAT AAGAAGGAGT ACATCGAGAG GATGGTGAAA 
TGGAGGATTG AGAGGGGTGT TGTGCAGCAA ACAGAGAGCT TAGTGCGTGG CTTTTATGAG 
GTCGTGGATG CCAGGCTGGT ATCTGTTTTT GATGCGAGAG AACTAGAACT GGTCATCGCA 
GGCACAGCTG AAATAGACCT AAGTGATTGG AGGAACAACA CAGAATATAG AGGAGGATAT 
CATGACAATC ATATTGTAAT TCGGTGGTTC TGGGCTGCAG TGGAAAGATT CAACAATGAA 
CAACGACTAA GGTTGTTACA GTTTGTTACA GGCACATCCA GCATTCCCTA TGAAGGATTT 
GCTTCTCTCC GAGGGAGTAA CGGCCCAAGA AGATTCTGTG TGGAGAAATG GGGGAAAATC 
ACCGCTCTTC CCAGTACAGA GAGCATTGTC TACTGGAGCT ATCAGCTTGG AAATTGGAAA 
GTAACTGAGG AGCTGGTATC ACTTTCTTTA ATTAAAAAAA AATCCAGAAC AAGTGATGGA 
CACTGGGAGG ATCGCTTGGT GACACCACCA CTTTTCCGTA TTGTGCCTGC CTTGGGACGT 
CCCCCCTTGG TCTCCCAGTT CCCCACCACC TGCTGGTCCC ACGGCAGTCT GCTCCATCAT 
TTAATCCCTG ATACAAGGCC CTCTTCCACC CTTTCCCTTT CTTTCCACTT ACTCAATTTA 
TACTGTCTTA TAATAATATT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019794790.1
CDS	2488..7011
Translation	MEAAEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGSQHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFRVEVTSSVHEDASPEAVGTILGVNTVNGDLGSPSDEEDMPGGHHDSTVCSNGPVSEESANGTPKHSFRTSSTLEIDSEEFTSTSSRTSPPRGRQDSLNDYLDAIEHNGHSRSGAATCSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRTDDGSLTSQIKPEDNTVENEEASTNEAASFEDKPESLPELAESSFPSGPVPDESENAPECQPSADQGSTELCGAQEVDQPTSGADTGTSDTSGGSRRAVSEMESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLDGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQGSICTPGSLPVVQVPSGEEEGPGAESATSPDQEEVGEVWQRRGSLEGAAAATESPPQEERDAGHAQGACEGATAQEEGATGGSQANGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTNAIDVAGEEADFHQASADFRRENVPPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPSTESIVYWSYQLGNWKVTEELVSLSLIKKKSRTSDGHWEDRLVTPPLFRIVPALGRPPLVSQFPTTCWSHGSLLHHLIPDTRPSSTLSLSFHLLNLYCLIIIF

Target ORF information:

RefSeq Version	XM_019939231.1
Organism	Tursiops truncatus(common bottlenose dolphin)
Definition	Tursiops truncatus E3 ubiquitin-protein ligase HECW2 (LOC101327442), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019939231.1

ORF Insert Sequence:

ATGGAAGCTG CGGAGATAAA AATCTGTTTC AAATACTACC ACGGCATTAG TGGAGCCCTG 
CGAGCCACGA CCCCCTGCAT CACGGTGAAG AACCCCGCTG TGATGATGGG GGCAGAAGGC 
ATGGAAGGAG GTGCTTCAGG AAGTCAGCAC TCTCGAAAAC TCGTTAGCTT TACGTTGTCT 
GATCTTCGGG CAGTTGGACT AAAGAAAGGG ATGTTCTTCA ATCCTGACCC TTATCTTAAG 
ATGTCGATTC AGCCAGGGAA GAAGAGCAGT TTCCCCACTT GTGCCCACCA TGGGCAGGAG 
AGGAGGTCTA CTATCATCAG TAACACCACA AATCCAATTT GGCACCGAGA GAAATATTCC 
TTTTTCGCAC TCCTTACTGA TGTCTTAGAA ATTGAAATTA AAGACAAATT TGCCAAGAGC 
CGACCTATCA TCAAGCGTTT TCTGGGGAAA CTAACCATTC CAGTCCAGAG GCTTTTGGAG 
CGACAAGCCA TCGGTGACCA GATGCTCAGC TACAACCTCG GCAGAAGGCT CCCAGCTGAC 
CACGTGAGTG GGTACCTCCA GTTCAGAGTG GAGGTCACAT CTTCTGTGCA TGAAGATGCT 
TCTCCAGAAG CTGTTGGCAC AATACTTGGA GTCAATACTG TGAATGGAGA CCTAGGAAGC 
CCTTCTGATG AGGAGGACAT GCCAGGGGGC CATCATGACA GCACAGTTTG TTCCAATGGA 
CCAGTTTCTG AGGAAAGTGC TAATGGGACC CCCAAGCATT CTTTCAGGAC TAGCTCTACT 
CTGGAAATAG ACTCAGAAGA ATTCACCTCT ACTTCTTCAA GGACCTCACC TCCCAGAGGA 
CGTCAGGATT CACTCAATGA TTACTTAGAT GCTATTGAAC ACAATGGCCA TTCCAGGTCG 
GGTGCAGCTA CTTGTTCGGA AAGGTCCATG GGAGCCTCTC CGAAACTAAG GAGTAGTTTT 
CCCACCGACA CAAGACTCAA TGCAATGCTT CATATCGACT CAGATGAAGA AGACCACGAG 
TTCCAGCAAG ACCTGGGTTA TCCATCCTCC TTGGAGGAGG AAGGAGGGTT GATAATGTTT 
AGCAGGGCGT CAAGAACTGA TGATGGGAGC CTGACATCTC AGATAAAGCC AGAAGACAAC 
ACGGTGGAGA ATGAGGAAGC CTCCACCAAT GAAGCTGCTT CCTTCGAGGA CAAGCCAGAA 
AGTCTTCCAG AGCTTGCGGA GAGCTCCTTC CCCTCAGGCC CAGTCCCTGA TGAAAGCGAG 
AATGCCCCGG AGTGTCAGCC GAGTGCTGAC CAGGGGAGCA CTGAATTGTG CGGCGCCCAG 
GAGGTAGATC AGCCCACAAG TGGGGCAGAC ACGGGCACTT CTGACACGTC AGGAGGAAGC 
CGAAGAGCCG TCAGTGAGAT GGAGTCCCTT GATCAGGGGT CTGAGCCTTC CCAGGTGTCC 
TCGGAAACAG AGCCCAGTGA CCCCGCCAGG ACAGAGAGCG TGAGCGAGGC CAGCACCAGG 
CCTGAGGGAG AGAGTGACCT GGACGGGGCC GACAGCTCCT GCAATGAGAG TGTCACCACG 
CAGCTGTCCT CGGTGGAGAC ACGGTGCTCA TCTCTTGAGA GTGCGCGGTT TCCCGAAACT 
CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT GGAGCCTGTG CGGCAGAGCC CACCAGCAGT 
GGCCCTGCTG AAGGGTCACA GGGTTCCATA TGCACTCCTG GTTCTTTACC TGTAGTGCAG 
GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG GCAGAATCGG CTACTTCACC TGACCAGGAG 
GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG AGCCTGGAGG GAGCTGCTGC TGCTACTGAG 
AGCCCGCCCC AAGAAGAGCG TGATGCGGGG CATGCCCAAG GGGCTTGTGA AGGGGCCACT 
GCCCAGGAGG AGGGCGCGAC CGGAGGTTCT CAAGCCAACG GCCACCAGCC TTTGCGATCA 
CTGCCTTCAG TGCGCCAGGA TGTTAGCCGG TACCAGAGGG TGGACGAGGC TCTCCCACCA 
AACTGGGAGG CACGCATTGA CAGCCATGGC AGGATCTTCT ACGTGGATCA CGTAAACAGA 
ACCACGACTT GGCAGCGGCC CACAGCTCCC CCTGCCCCGC AGGTGCTGCA GAGGTCCAAC 
TCCATACAGC AGATGGAGCA GCTGAACCGG CGATACCAGA GTATCCGCAG AACCATGACA 
AACGAGAGGC CTGAGGAAAA CACCAATGCG ATTGATGTGG CAGGGGAGGA AGCCGACTTT 
CACCAAGCCA GTGCAGATTT CCGACGGGAG AACGTCCCGC CCCACTCTAC CTCCAGATCC 
AGGCTCACGT TGCTGTTACA GTCTCCCCCC GTGAAGTTCC TCATCAGCCC AGAGTTCTTC 
ACTGTGCTGC ATTCTAACCC TAGTGCCTAC CGCATGTTTA CAAACAACAC GTGTTTGAAG 
CACATGATCA CCAAAGTCCG GCGGGACACC CACCACTTTG AACGCTACCA GCATAACCGG 
GACCTTGTGG GATTCCTCAA CATGTTTGCA AACAAACAAC TGGAGCTGCC GAGGGGCTGG 
GAAATGAAAC ATGATCATCA GGGCAAGGCA TTTTTTGTCG ACCACAACTC GCGTACCACC 
ACCTTCATCG ATCCCCGGCT CCCGCTTCAG AGCAGCAGAC CCACGAGCGC GCTGGTGCAT 
CGACAGCACC TGACCAGGCA GCGCAGCCAC AGCGCAGGCG AGGTAGGAGA AGATTCTCGA 
CATGCAGGAC CACCAGTTCT TCCCAGGCCA TCAAGTACGT TCAATACAGT TAGTAGGCCA 
CAGTACCAGG ACATGGTTCC AGTGGCTTAC AATGACAAGA TTGTTGCATT TCTGCGCCAG 
CCCAACATCT TTGAAATTCT ACAGGAGCGT CAACCTGATC TTACCAGGAA TCACTCACTC 
AGGGAGAAGA TCCAATTTAT CCGAACTGAA GGGACCCCAG GATTGGTGCG TCTTTCAAGT 
GATGCCGACC TTGTTATGTT GCTGAGTTTA TTTGAAGAAG AGATAATGTC ATATGTGCCT 
CCACATGCCT TACTCCATCC CAGCTACTGT CAGTCCCCAC GTGGCTCTCC TGTGTCCTCT 
CCCCAGAACT CACCAGGTAC TCAGCGTGCC AATGCCCGTG CTCCAGCCCC TTACAAGCGA 
GACTTTGAAG CCAAACTAAG AAACTTTTAC AGGAAGTTAG AGACTAAAGG ATATGGACAA 
GGCCCAGGGA AATTAAAGTT AATTATCAGA AGAGATCACT TGCTCGAAGA TGCTTTTAAT 
CAGATTATGG GCTACTCCAG AAAAGACCTG CAGAGAAATA AGCTGTATGT CACATTTGTT 
GGGGAGGAAG GGCTGGATTA CAGTGGACCT TCCAGAGAGT TTTTCTTCCT GGTATCCAGA 
GAACTCTTTA ACCCATATTA TGGCTTATTT GAATATTCAG CCAATGACAC ATACACAGTA 
CAAATAAGTC CCATGTCTGC TTTTGTAGAC AATCACCATG AATGGTTCCG GTTCAGTGGT 
AGGATCCTTG GTCTTGCACT AATTCACCAG TATTTGTTGG ATGCCTTCTT CACACGGCCC 
TTTTATAAGG CTCTTCTCAG AATTCTCTGT GACCTGAGTG ATCTAGAATA CCTTGATGAA 
GAATTTCACC AGAGCCTGCA GTGGATGAAA GACAATGACA TCCATGACAT CCTGGACCTC 
ACGTTCACTG TGAACGAAGA AGTTTTTGGG CAGATTACCG AACGAGAATT AAAACCAGGG 
GGTGCTAACA TCCCAGTCAC GGAGAAGAAT AAGAAGGAGT ACATCGAGAG GATGGTGAAA 
TGGAGGATTG AGAGGGGTGT TGTGCAGCAA ACAGAGAGCT TAGTGCGTGG CTTTTATGAG 
GTCGTGGATG CCAGGCTGGT ATCTGTTTTT GATGCGAGAG AACTAGAACT GGTCATCGCA 
GGCACAGCTG AAATAGACCT AAGTGATTGG AGGAACAACA CAGAATATAG AGGAGGATAT 
CATGACAATC ATATTGTAAT TCGGTGGTTC TGGGCTGCAG TGGAAAGATT CAACAATGAA 
CAACGACTAA GGTTGTTACA GTTTGTTACA GGCACATCCA GCATTCCCTA TGAAGGATTT 
GCTTCTCTCC GAGGGAGTAA CGGCCCAAGA AGATTCTGTG TGGAGAAATG GGGGAAAATC 
ACCGCTCTTC CCAGTACAGA GAGCATTGTC TACTGGAGCT ATCAGCTTGG AAATTGGAAA 
GTAACTGAGG AGCTGGTATC ACTTTCTTTA ATTAAAAAAA AATCCAGAAC AAGTGATGGA 
CACTGGGAGG ATCGCTTGGT GACACCACCA CTTTTCCGTA TTGTGCCTGC CTTGGGACGT 
CCCCCCTTGG TCTCCCAGTT CCCCACCACC TGCTGGTCCC ACGGCAGTCT GCTCCATCAT 
TTAATCCCTG ATACAAGGCC CTCTTCCACC CTTTCCCTTT CTTTCCACTT ACTCAATTTA 
TACTGTCTTA TAATAATATT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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電話

03-6811-6572

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03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

LOC101327442 cDNA ORF clone, Tursiops truncatus(common bottlenose dolphin)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム