NEDD4 cDNA ORF clone, Canis lupus familiaris(dog)

The following NEDD4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEDD4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe28895 XM_005638438.2
Latest version!
Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe28896 XM_005638439.2
Latest version!
Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe28897 XM_022412612.1
Latest version!
Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe28897 XM_005638440.2
Latest version!
Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe28898 XM_022412613.1
Latest version!
Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe28898 XM_022412614.1
Latest version!
Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe28898 XM_005638441.3
Latest version!
Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe28893 XM_005638437.2
Latest version!
Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
OCe28898 XM_005638441.2 Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe28893 XM_005638437.1 Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe28895
    Clone ID Related Accession (Same CDS sequence) XM_005638438.2
    Accession Version XM_005638438.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2634bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase NEDD4 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005638438.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAAGAAAT ATTATTCAGA GTCCATCCTC AGCAGCACCG GCTTCTTTTT GAAGTATTTG 
    ATGAAAATCG ATTGCCTCAA GCACAATTGT TCTAAGGGGG GACCATCACA ATTACTCATA
    AAATTTGCAC CTGGAAATGA AGATAAAGTT GATAACTTAT CAAGAGACAG TAACAGAGAT
    TTCACAAATG AACTATCTAA TTCTTTAAAG CATTCTTGTA AGACCAGAGA TGATTTCCTA
    GGTCAAGTGG ATGTTCCTCT TTATCCATTA CCGACAGAAA ATCCAAGAAT GGAGAGACCA
    TATACGTTTA AGGATTTTGT TCTTCACCCA AGAAGTCACA AATCAAGAGT TAAAGGTTAT
    TTGAGATTAA AAATGACTTA TTTACCTAAA ACCAGTGGCT CAGAAGAAGA TAACGCAGAA
    CAGGCCGAGG AACTAGAGCC TGGCTGGGTT GTTTTGGACC AACCGGATGC TGCCTGCCAT
    TTGCAGCAGC AGCAAGAACC TTCTCCTCTA CCTCCAGGAT GGGAAGAGAG GCAGGATATT
    CTTGGAAGGA CCTACTATGT AAACCATGAA TCTAGAAGAA CACAGTGGAA AAGACCAACC
    GCTCAGGACA ACCTAACTGA TGCTGAGAAT GGTAACATGC AACTGCAAGC ACAGCGTGCA
    TTTACTACCA GACGGCAGAT ATCTGAGGAA ACAGAAAGTG TGGATAATCG AGAGTCTTCC
    GAGAGCTGGG AAATTATAAG AGAAGATGAA GCCACCATGT ATAGCAATCA GGCCTTCCCA
    TCACCTCCAG CATCAAATAA CTTGGATGTT CAGACTCATC TTGCAGAAGA ATTGAATGCC
    AGACTCACAA TTTGTAGAAA CTCAGCCACC AGCCAGCCAG TATCCAGCTC AAATCATTCC
    AGCAGAAGAG GCAGCTTACA AGCCTATACT TTTGAGGAAC AGCCTACACT TCCTGTGCTT
    TTGCCTACTT CATCTGGATT ACCACCAGGT TGGGAGGAAA AACAAGATGA AAGAGGAAGA
    TCATATTATG TAGATCACAA TTCCAGAACT ACTACCTGGA CAAAGCCCGT TGTACAGGCC
    ACGGTGGAGA CCAGTCAGCT GCAGCCAAGC CAGAGTGCGG CATGTCCTCA ACCACAGGTC
    CCCTTGAGTG AGTCAGCGCA GCAGGGGACC CAGGCGGCTG AAATGGAGCA AGGATTCCTC
    CCTAAAGGCT GGGAAGTCCG GCATGCGCCA AATGGGAGGC CTTTCTTTAT TGACCACAAC
    ACCAAAACCA CTACCTGGGA AGATCCAAGA CTGAAAATTC CAGCTCATCT GAGAGGAAAG
    ATGTCACTGG ATTCTTCCAA TGATCTGGGG CCTTTACCTC CAGGATGGGA AGAAAGAACT
    CATACAGATG GAAGAATCTT CTACATAAAT CACAATATAA AAAGAACACA ATGGGAAGAT
    CCTCGGTTGC AAAATGTAGC AATAACTGGA CCAGCAGTGC CCTACTCCAG GGATTACAAA
    AGAAAGTATG AGTTCTTCCG ACGAAAGTTG AAGAAGCAGA ACGACATTCC AAACAAATTT
    GAAATGAAAC TTCGCCGTGC AACTGTTCTT GAGGACTCTT ACAGGAGGAT TATGGGTGTC
    AAGAGAGCGG ATTTCCTCAA GGCTCGACTG TGGATTGAGT TCGATGGTGA AAAGGGATTA
    GATTACGGAG GAGTCGCTAG AGAATGGTTT TTCCTGATCT CAAAGGAAAT GTTTAACCCT
    TATTATGGGT TGTTTGAATA TTCTGCTACG GATAATTACA CCCTACAGAT AAATCCAAAC
    TCTGGATTGT GTAACGAGGA TCACCTCTCT TACTTCAAGT TTATTGGCCG AGTAGCTGGA
    ATGGCAGTTT ATCATGGCAA ACTGTTGGAT GGTTTCTTCA TCCGCCCATT TTACAAGATG
    ATGCTACACA AACCAATAAC ACTTCATGAT ATGGAATCGG TGGATGGTGA ATATTACAAT
    TCACTAAGAT GGATTCTTGA AAATGATCCA ACAGAATTGG ACCTCAGGTT TGTCATAGAT
    GAAGAACTTT TTGGACAGAC ACATCAACAT GAATTGAAAA TTGGTGGATC AGAAATAGTT
    GTCACCAATA AGAACAAAAA GGAATATATT TATCTTGTAA TACAGTGGCG ATTTGTAAAC
    CGAATCCAGA AGCAAATGGC TGCTTTTAAA GAGGGATTTT TTGAACTAAT ACCACAGGAT
    CTCATCAAAA TTTTCGATGA AAATGAACTA GAGCTTCTTA TGTGTGGATT GGGAGATGTC
    GATGTGAATG ACTGGAGAGA ACACACAAAA TATAAGAATG GCTACAGTGT GAATCATCAA
    GTCATACAGT GGTTCTGGAA GGCTGTTCTA ATGATGGATT CAGAAAAAAG AATAAGATTA
    CTTCAGTTTG TCACTGGTAC ATCTCGAGTG CCTATGAATG GATTTGCTGA ACTCTATGGC
    TCAAATGGAC CACAGTCATT TACAGTTGAA CAGTGGGGTA CCCCGGAAAA GCTGCCAAGA
    GCCCATACAT GCTTTAATCG CTTGGACTTG CCACCCTATG AATCATTTGA AGAATTATGG
    GATAAACTGC AGATGGCAAT TGAGAACACT CAAGGCTTTG ATGGCGTTGA TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005638495.1
    CDS96..2729
    Translation

    Target ORF information:

    RefSeq Version XM_005638438.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005638438.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAAGAAAT ATTATTCAGA GTCCATCCTC AGCAGCACCG GCTTCTTTTT GAAGTATTTG 
    ATGAAAATCG ATTGCCTCAA GCACAATTGT TCTAAGGGGG GACCATCACA ATTACTCATA
    AAATTTGCAC CTGGAAATGA AGATAAAGTT GATAACTTAT CAAGAGACAG TAACAGAGAT
    TTCACAAATG AACTATCTAA TTCTTTAAAG CATTCTTGTA AGACCAGAGA TGATTTCCTA
    GGTCAAGTGG ATGTTCCTCT TTATCCATTA CCGACAGAAA ATCCAAGAAT GGAGAGACCA
    TATACGTTTA AGGATTTTGT TCTTCACCCA AGAAGTCACA AATCAAGAGT TAAAGGTTAT
    TTGAGATTAA AAATGACTTA TTTACCTAAA ACCAGTGGCT CAGAAGAAGA TAACGCAGAA
    CAGGCCGAGG AACTAGAGCC TGGCTGGGTT GTTTTGGACC AACCGGATGC TGCCTGCCAT
    TTGCAGCAGC AGCAAGAACC TTCTCCTCTA CCTCCAGGAT GGGAAGAGAG GCAGGATATT
    CTTGGAAGGA CCTACTATGT AAACCATGAA TCTAGAAGAA CACAGTGGAA AAGACCAACC
    GCTCAGGACA ACCTAACTGA TGCTGAGAAT GGTAACATGC AACTGCAAGC ACAGCGTGCA
    TTTACTACCA GACGGCAGAT ATCTGAGGAA ACAGAAAGTG TGGATAATCG AGAGTCTTCC
    GAGAGCTGGG AAATTATAAG AGAAGATGAA GCCACCATGT ATAGCAATCA GGCCTTCCCA
    TCACCTCCAG CATCAAATAA CTTGGATGTT CAGACTCATC TTGCAGAAGA ATTGAATGCC
    AGACTCACAA TTTGTAGAAA CTCAGCCACC AGCCAGCCAG TATCCAGCTC AAATCATTCC
    AGCAGAAGAG GCAGCTTACA AGCCTATACT TTTGAGGAAC AGCCTACACT TCCTGTGCTT
    TTGCCTACTT CATCTGGATT ACCACCAGGT TGGGAGGAAA AACAAGATGA AAGAGGAAGA
    TCATATTATG TAGATCACAA TTCCAGAACT ACTACCTGGA CAAAGCCCGT TGTACAGGCC
    ACGGTGGAGA CCAGTCAGCT GCAGCCAAGC CAGAGTGCGG CATGTCCTCA ACCACAGGTC
    CCCTTGAGTG AGTCAGCGCA GCAGGGGACC CAGGCGGCTG AAATGGAGCA AGGATTCCTC
    CCTAAAGGCT GGGAAGTCCG GCATGCGCCA AATGGGAGGC CTTTCTTTAT TGACCACAAC
    ACCAAAACCA CTACCTGGGA AGATCCAAGA CTGAAAATTC CAGCTCATCT GAGAGGAAAG
    ATGTCACTGG ATTCTTCCAA TGATCTGGGG CCTTTACCTC CAGGATGGGA AGAAAGAACT
    CATACAGATG GAAGAATCTT CTACATAAAT CACAATATAA AAAGAACACA ATGGGAAGAT
    CCTCGGTTGC AAAATGTAGC AATAACTGGA CCAGCAGTGC CCTACTCCAG GGATTACAAA
    AGAAAGTATG AGTTCTTCCG ACGAAAGTTG AAGAAGCAGA ACGACATTCC AAACAAATTT
    GAAATGAAAC TTCGCCGTGC AACTGTTCTT GAGGACTCTT ACAGGAGGAT TATGGGTGTC
    AAGAGAGCGG ATTTCCTCAA GGCTCGACTG TGGATTGAGT TCGATGGTGA AAAGGGATTA
    GATTACGGAG GAGTCGCTAG AGAATGGTTT TTCCTGATCT CAAAGGAAAT GTTTAACCCT
    TATTATGGGT TGTTTGAATA TTCTGCTACG GATAATTACA CCCTACAGAT AAATCCAAAC
    TCTGGATTGT GTAACGAGGA TCACCTCTCT TACTTCAAGT TTATTGGCCG AGTAGCTGGA
    ATGGCAGTTT ATCATGGCAA ACTGTTGGAT GGTTTCTTCA TCCGCCCATT TTACAAGATG
    ATGCTACACA AACCAATAAC ACTTCATGAT ATGGAATCGG TGGATGGTGA ATATTACAAT
    TCACTAAGAT GGATTCTTGA AAATGATCCA ACAGAATTGG ACCTCAGGTT TGTCATAGAT
    GAAGAACTTT TTGGACAGAC ACATCAACAT GAATTGAAAA TTGGTGGATC AGAAATAGTT
    GTCACCAATA AGAACAAAAA GGAATATATT TATCTTGTAA TACAGTGGCG ATTTGTAAAC
    CGAATCCAGA AGCAAATGGC TGCTTTTAAA GAGGGATTTT TTGAACTAAT ACCACAGGAT
    CTCATCAAAA TTTTCGATGA AAATGAACTA GAGCTTCTTA TGTGTGGATT GGGAGATGTC
    GATGTGAATG ACTGGAGAGA ACACACAAAA TATAAGAATG GCTACAGTGT GAATCATCAA
    GTCATACAGT GGTTCTGGAA GGCTGTTCTA ATGATGGATT CAGAAAAAAG AATAAGATTA
    CTTCAGTTTG TCACTGGTAC ATCTCGAGTG CCTATGAATG GATTTGCTGA ACTCTATGGC
    TCAAATGGAC CACAGTCATT TACAGTTGAA CAGTGGGGTA CCCCGGAAAA GCTGCCAAGA
    GCCCATACAT GCTTTAATCG CTTGGACTTG CCACCCTATG AATCATTTGA AGAATTATGG
    GATAAACTGC AGATGGCAAT TGAGAACACT CAAGGCTTTG ATGGCGTTGA TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe28896
    Clone ID Related Accession (Same CDS sequence) XM_005638439.2
    Accession Version XM_005638439.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2574bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase NEDD4 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005638439.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAAATCG ATTGCCTCAA GCACAATTGT TCTAAGGGGG GACCATCACA ATTACTCATA 
    AAATTTGCAC CTGGAAATGA AGATAAAGTT GATAACTTAT CAAGAGACAG TAACAGAGAT
    TTCACAAATG AACTATCTAA TTCTTTAAAG CATTCTTGTA AGACCAGAGA TGATTTCCTA
    GGTCAAGTGG ATGTTCCTCT TTATCCATTA CCGACAGAAA ATCCAAGAAT GGAGAGACCA
    TATACGTTTA AGGATTTTGT TCTTCACCCA AGAAGTCACA AATCAAGAGT TAAAGGTTAT
    TTGAGATTAA AAATGACTTA TTTACCTAAA ACCAGTGGCT CAGAAGAAGA TAACGCAGAA
    CAGGCCGAGG AACTAGAGCC TGGCTGGGTT GTTTTGGACC AACCGGATGC TGCCTGCCAT
    TTGCAGCAGC AGCAAGAACC TTCTCCTCTA CCTCCAGGAT GGGAAGAGAG GCAGGATATT
    CTTGGAAGGA CCTACTATGT AAACCATGAA TCTAGAAGAA CACAGTGGAA AAGACCAACC
    GCTCAGGACA ACCTAACTGA TGCTGAGAAT GGTAACATGC AACTGCAAGC ACAGCGTGCA
    TTTACTACCA GACGGCAGAT ATCTGAGGAA ACAGAAAGTG TGGATAATCG AGAGTCTTCC
    GAGAGCTGGG AAATTATAAG AGAAGATGAA GCCACCATGT ATAGCAATCA GGCCTTCCCA
    TCACCTCCAG CATCAAATAA CTTGGATGTT CAGACTCATC TTGCAGAAGA ATTGAATGCC
    AGACTCACAA TTTGTAGAAA CTCAGCCACC AGCCAGCCAG TATCCAGCTC AAATCATTCC
    AGCAGAAGAG GCAGCTTACA AGCCTATACT TTTGAGGAAC AGCCTACACT TCCTGTGCTT
    TTGCCTACTT CATCTGGATT ACCACCAGGT TGGGAGGAAA AACAAGATGA AAGAGGAAGA
    TCATATTATG TAGATCACAA TTCCAGAACT ACTACCTGGA CAAAGCCCGT TGTACAGGCC
    ACGGTGGAGA CCAGTCAGCT GCAGCCAAGC CAGAGTGCGG CATGTCCTCA ACCACAGGTC
    CCCTTGAGTG AGTCAGCGCA GCAGGGGACC CAGGCGGCTG AAATGGAGCA AGGATTCCTC
    CCTAAAGGCT GGGAAGTCCG GCATGCGCCA AATGGGAGGC CTTTCTTTAT TGACCACAAC
    ACCAAAACCA CTACCTGGGA AGATCCAAGA CTGAAAATTC CAGCTCATCT GAGAGGAAAG
    ATGTCACTGG ATTCTTCCAA TGATCTGGGG CCTTTACCTC CAGGATGGGA AGAAAGAACT
    CATACAGATG GAAGAATCTT CTACATAAAT CACAATATAA AAAGAACACA ATGGGAAGAT
    CCTCGGTTGC AAAATGTAGC AATAACTGGA CCAGCAGTGC CCTACTCCAG GGATTACAAA
    AGAAAGTATG AGTTCTTCCG ACGAAAGTTG AAGAAGCAGA ACGACATTCC AAACAAATTT
    GAAATGAAAC TTCGCCGTGC AACTGTTCTT GAGGACTCTT ACAGGAGGAT TATGGGTGTC
    AAGAGAGCGG ATTTCCTCAA GGCTCGACTG TGGATTGAGT TCGATGGTGA AAAGGGATTA
    GATTACGGAG GAGTCGCTAG AGAATGGTTT TTCCTGATCT CAAAGGAAAT GTTTAACCCT
    TATTATGGGT TGTTTGAATA TTCTGCTACG GATAATTACA CCCTACAGAT AAATCCAAAC
    TCTGGATTGT GTAACGAGGA TCACCTCTCT TACTTCAAGT TTATTGGCCG AGTAGCTGGA
    ATGGCAGTTT ATCATGGCAA ACTGTTGGAT GGTTTCTTCA TCCGCCCATT TTACAAGATG
    ATGCTACACA AACCAATAAC ACTTCATGAT ATGGAATCGG TGGATGGTGA ATATTACAAT
    TCACTAAGAT GGATTCTTGA AAATGATCCA ACAGAATTGG ACCTCAGGTT TGTCATAGAT
    GAAGAACTTT TTGGACAGAC ACATCAACAT GAATTGAAAA TTGGTGGATC AGAAATAGTT
    GTCACCAATA AGAACAAAAA GGAATATATT TATCTTGTAA TACAGTGGCG ATTTGTAAAC
    CGAATCCAGA AGCAAATGGC TGCTTTTAAA GAGGGATTTT TTGAACTAAT ACCACAGGAT
    CTCATCAAAA TTTTCGATGA AAATGAACTA GAGCTTCTTA TGTGTGGATT GGGAGATGTC
    GATGTGAATG ACTGGAGAGA ACACACAAAA TATAAGAATG GCTACAGTGT GAATCATCAA
    GTCATACAGT GGTTCTGGAA GGCTGTTCTA ATGATGGATT CAGAAAAAAG AATAAGATTA
    CTTCAGTTTG TCACTGGTAC ATCTCGAGTG CCTATGAATG GATTTGCTGA ACTCTATGGC
    TCAAATGGAC CACAGTCATT TACAGTTGAA CAGTGGGGTA CCCCGGAAAA GCTGCCAAGA
    GCCCATACAT GCTTTAATCG CTTGGACTTG CCACCCTATG AATCATTTGA AGAATTATGG
    GATAAACTGC AGATGGCAAT TGAGAACACT CAAGGCTTTG ATGGCGTTGA TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005638496.1
    CDS132..2705
    Translation

    Target ORF information:

    RefSeq Version XM_005638439.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005638439.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAAATCG ATTGCCTCAA GCACAATTGT TCTAAGGGGG GACCATCACA ATTACTCATA 
    AAATTTGCAC CTGGAAATGA AGATAAAGTT GATAACTTAT CAAGAGACAG TAACAGAGAT
    TTCACAAATG AACTATCTAA TTCTTTAAAG CATTCTTGTA AGACCAGAGA TGATTTCCTA
    GGTCAAGTGG ATGTTCCTCT TTATCCATTA CCGACAGAAA ATCCAAGAAT GGAGAGACCA
    TATACGTTTA AGGATTTTGT TCTTCACCCA AGAAGTCACA AATCAAGAGT TAAAGGTTAT
    TTGAGATTAA AAATGACTTA TTTACCTAAA ACCAGTGGCT CAGAAGAAGA TAACGCAGAA
    CAGGCCGAGG AACTAGAGCC TGGCTGGGTT GTTTTGGACC AACCGGATGC TGCCTGCCAT
    TTGCAGCAGC AGCAAGAACC TTCTCCTCTA CCTCCAGGAT GGGAAGAGAG GCAGGATATT
    CTTGGAAGGA CCTACTATGT AAACCATGAA TCTAGAAGAA CACAGTGGAA AAGACCAACC
    GCTCAGGACA ACCTAACTGA TGCTGAGAAT GGTAACATGC AACTGCAAGC ACAGCGTGCA
    TTTACTACCA GACGGCAGAT ATCTGAGGAA ACAGAAAGTG TGGATAATCG AGAGTCTTCC
    GAGAGCTGGG AAATTATAAG AGAAGATGAA GCCACCATGT ATAGCAATCA GGCCTTCCCA
    TCACCTCCAG CATCAAATAA CTTGGATGTT CAGACTCATC TTGCAGAAGA ATTGAATGCC
    AGACTCACAA TTTGTAGAAA CTCAGCCACC AGCCAGCCAG TATCCAGCTC AAATCATTCC
    AGCAGAAGAG GCAGCTTACA AGCCTATACT TTTGAGGAAC AGCCTACACT TCCTGTGCTT
    TTGCCTACTT CATCTGGATT ACCACCAGGT TGGGAGGAAA AACAAGATGA AAGAGGAAGA
    TCATATTATG TAGATCACAA TTCCAGAACT ACTACCTGGA CAAAGCCCGT TGTACAGGCC
    ACGGTGGAGA CCAGTCAGCT GCAGCCAAGC CAGAGTGCGG CATGTCCTCA ACCACAGGTC
    CCCTTGAGTG AGTCAGCGCA GCAGGGGACC CAGGCGGCTG AAATGGAGCA AGGATTCCTC
    CCTAAAGGCT GGGAAGTCCG GCATGCGCCA AATGGGAGGC CTTTCTTTAT TGACCACAAC
    ACCAAAACCA CTACCTGGGA AGATCCAAGA CTGAAAATTC CAGCTCATCT GAGAGGAAAG
    ATGTCACTGG ATTCTTCCAA TGATCTGGGG CCTTTACCTC CAGGATGGGA AGAAAGAACT
    CATACAGATG GAAGAATCTT CTACATAAAT CACAATATAA AAAGAACACA ATGGGAAGAT
    CCTCGGTTGC AAAATGTAGC AATAACTGGA CCAGCAGTGC CCTACTCCAG GGATTACAAA
    AGAAAGTATG AGTTCTTCCG ACGAAAGTTG AAGAAGCAGA ACGACATTCC AAACAAATTT
    GAAATGAAAC TTCGCCGTGC AACTGTTCTT GAGGACTCTT ACAGGAGGAT TATGGGTGTC
    AAGAGAGCGG ATTTCCTCAA GGCTCGACTG TGGATTGAGT TCGATGGTGA AAAGGGATTA
    GATTACGGAG GAGTCGCTAG AGAATGGTTT TTCCTGATCT CAAAGGAAAT GTTTAACCCT
    TATTATGGGT TGTTTGAATA TTCTGCTACG GATAATTACA CCCTACAGAT AAATCCAAAC
    TCTGGATTGT GTAACGAGGA TCACCTCTCT TACTTCAAGT TTATTGGCCG AGTAGCTGGA
    ATGGCAGTTT ATCATGGCAA ACTGTTGGAT GGTTTCTTCA TCCGCCCATT TTACAAGATG
    ATGCTACACA AACCAATAAC ACTTCATGAT ATGGAATCGG TGGATGGTGA ATATTACAAT
    TCACTAAGAT GGATTCTTGA AAATGATCCA ACAGAATTGG ACCTCAGGTT TGTCATAGAT
    GAAGAACTTT TTGGACAGAC ACATCAACAT GAATTGAAAA TTGGTGGATC AGAAATAGTT
    GTCACCAATA AGAACAAAAA GGAATATATT TATCTTGTAA TACAGTGGCG ATTTGTAAAC
    CGAATCCAGA AGCAAATGGC TGCTTTTAAA GAGGGATTTT TTGAACTAAT ACCACAGGAT
    CTCATCAAAA TTTTCGATGA AAATGAACTA GAGCTTCTTA TGTGTGGATT GGGAGATGTC
    GATGTGAATG ACTGGAGAGA ACACACAAAA TATAAGAATG GCTACAGTGT GAATCATCAA
    GTCATACAGT GGTTCTGGAA GGCTGTTCTA ATGATGGATT CAGAAAAAAG AATAAGATTA
    CTTCAGTTTG TCACTGGTAC ATCTCGAGTG CCTATGAATG GATTTGCTGA ACTCTATGGC
    TCAAATGGAC CACAGTCATT TACAGTTGAA CAGTGGGGTA CCCCGGAAAA GCTGCCAAGA
    GCCCATACAT GCTTTAATCG CTTGGACTTG CCACCCTATG AATCATTTGA AGAATTATGG
    GATAAACTGC AGATGGCAAT TGAGAACACT CAAGGCTTTG ATGGCGTTGA TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe28897
    Clone ID Related Accession (Same CDS sequence) XM_005638440.2 , XM_022412612.1
    Accession Version XM_005638440.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2550bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase NEDD4 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005638440.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAATGGAG TTTTTACAAG TATGCAAACG AAAACCATTA AAAAGACTTT GAATCCAAAA 
    TGGAATGAAG AAATATTATT CAGAGTCCAT CCTCAGCAGC ACCGGCTTCT TTTTGAAGTA
    TTTGATGAAA ATCGATTGAC CAGAGATGAT TTCCTAGGTC AAGTGGATGT TCCTCTTTAT
    CCATTACCGA CAGAAAATCC AAGAATGGAG AGACCATATA CGTTTAAGGA TTTTGTTCTT
    CACCCAAGAA GTCACAAATC AAGAGTTAAA GGTTATTTGA GATTAAAAAT GACTTATTTA
    CCTAAAACCA GTGGCTCAGA AGAAGATAAC GCAGAACAGG CCGAGGAACT AGAGCCTGGC
    TGGGTTGTTT TGGACCAACC GGATGCTGCC TGCCATTTGC AGCAGCAGCA AGAACCTTCT
    CCTCTACCTC CAGGATGGGA AGAGAGGCAG GATATTCTTG GAAGGACCTA CTATGTAAAC
    CATGAATCTA GAAGAACACA GTGGAAAAGA CCAACCGCTC AGGACAACCT AACTGATGCT
    GAGAATGGTA ACATGCAACT GCAAGCACAG CGTGCATTTA CTACCAGACG GCAGATATCT
    GAGGAAACAG AAAGTGTGGA TAATCGAGAG TCTTCCGAGA GCTGGGAAAT TATAAGAGAA
    GATGAAGCCA CCATGTATAG CAATCAGGCC TTCCCATCAC CTCCAGCATC AAATAACTTG
    GATGTTCAGA CTCATCTTGC AGAAGAATTG AATGCCAGAC TCACAATTTG TAGAAACTCA
    GCCACCAGCC AGCCAGTATC CAGCTCAAAT CATTCCAGCA GAAGAGGCAG CTTACAAGCC
    TATACTTTTG AGGAACAGCC TACACTTCCT GTGCTTTTGC CTACTTCATC TGGATTACCA
    CCAGGTTGGG AGGAAAAACA AGATGAAAGA GGAAGATCAT ATTATGTAGA TCACAATTCC
    AGAACTACTA CCTGGACAAA GCCCGTTGTA CAGGCCACGG TGGAGACCAG TCAGCTGCAG
    CCAAGCCAGA GTGCGGCATG TCCTCAACCA CAGGTCCCCT TGAGTGAGTC AGCGCAGCAG
    GGGACCCAGG CGGCTGAAAT GGAGCAAGGA TTCCTCCCTA AAGGCTGGGA AGTCCGGCAT
    GCGCCAAATG GGAGGCCTTT CTTTATTGAC CACAACACCA AAACCACTAC CTGGGAAGAT
    CCAAGACTGA AAATTCCAGC TCATCTGAGA GGAAAGATGT CACTGGATTC TTCCAATGAT
    CTGGGGCCTT TACCTCCAGG ATGGGAAGAA AGAACTCATA CAGATGGAAG AATCTTCTAC
    ATAAATCACA ATATAAAAAG AACACAATGG GAAGATCCTC GGTTGCAAAA TGTAGCAATA
    ACTGGACCAG CAGTGCCCTA CTCCAGGGAT TACAAAAGAA AGTATGAGTT CTTCCGACGA
    AAGTTGAAGA AGCAGAACGA CATTCCAAAC AAATTTGAAA TGAAACTTCG CCGTGCAACT
    GTTCTTGAGG ACTCTTACAG GAGGATTATG GGTGTCAAGA GAGCGGATTT CCTCAAGGCT
    CGACTGTGGA TTGAGTTCGA TGGTGAAAAG GGATTAGATT ACGGAGGAGT CGCTAGAGAA
    TGGTTTTTCC TGATCTCAAA GGAAATGTTT AACCCTTATT ATGGGTTGTT TGAATATTCT
    GCTACGGATA ATTACACCCT ACAGATAAAT CCAAACTCTG GATTGTGTAA CGAGGATCAC
    CTCTCTTACT TCAAGTTTAT TGGCCGAGTA GCTGGAATGG CAGTTTATCA TGGCAAACTG
    TTGGATGGTT TCTTCATCCG CCCATTTTAC AAGATGATGC TACACAAACC AATAACACTT
    CATGATATGG AATCGGTGGA TGGTGAATAT TACAATTCAC TAAGATGGAT TCTTGAAAAT
    GATCCAACAG AATTGGACCT CAGGTTTGTC ATAGATGAAG AACTTTTTGG ACAGACACAT
    CAACATGAAT TGAAAATTGG TGGATCAGAA ATAGTTGTCA CCAATAAGAA CAAAAAGGAA
    TATATTTATC TTGTAATACA GTGGCGATTT GTAAACCGAA TCCAGAAGCA AATGGCTGCT
    TTTAAAGAGG GATTTTTTGA ACTAATACCA CAGGATCTCA TCAAAATTTT CGATGAAAAT
    GAACTAGAGC TTCTTATGTG TGGATTGGGA GATGTCGATG TGAATGACTG GAGAGAACAC
    ACAAAATATA AGAATGGCTA CAGTGTGAAT CATCAAGTCA TACAGTGGTT CTGGAAGGCT
    GTTCTAATGA TGGATTCAGA AAAAAGAATA AGATTACTTC AGTTTGTCAC TGGTACATCT
    CGAGTGCCTA TGAATGGATT TGCTGAACTC TATGGCTCAA ATGGACCACA GTCATTTACA
    GTTGAACAGT GGGGTACCCC GGAAAAGCTG CCAAGAGCCC ATACATGCTT TAATCGCTTG
    GACTTGCCAC CCTATGAATC ATTTGAAGAA TTATGGGATA AACTGCAGAT GGCAATTGAG
    AACACTCAAG GCTTTGATGG CGTTGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005638497.1
    CDS128..2677
    Translation

    Target ORF information:

    RefSeq Version XM_005638440.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005638440.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAATGGAG TTTTTACAAG TATGCAAACG AAAACCATTA AAAAGACTTT GAATCCAAAA 
    TGGAATGAAG AAATATTATT CAGAGTCCAT CCTCAGCAGC ACCGGCTTCT TTTTGAAGTA
    TTTGATGAAA ATCGATTGAC CAGAGATGAT TTCCTAGGTC AAGTGGATGT TCCTCTTTAT
    CCATTACCGA CAGAAAATCC AAGAATGGAG AGACCATATA CGTTTAAGGA TTTTGTTCTT
    CACCCAAGAA GTCACAAATC AAGAGTTAAA GGTTATTTGA GATTAAAAAT GACTTATTTA
    CCTAAAACCA GTGGCTCAGA AGAAGATAAC GCAGAACAGG CCGAGGAACT AGAGCCTGGC
    TGGGTTGTTT TGGACCAACC GGATGCTGCC TGCCATTTGC AGCAGCAGCA AGAACCTTCT
    CCTCTACCTC CAGGATGGGA AGAGAGGCAG GATATTCTTG GAAGGACCTA CTATGTAAAC
    CATGAATCTA GAAGAACACA GTGGAAAAGA CCAACCGCTC AGGACAACCT AACTGATGCT
    GAGAATGGTA ACATGCAACT GCAAGCACAG CGTGCATTTA CTACCAGACG GCAGATATCT
    GAGGAAACAG AAAGTGTGGA TAATCGAGAG TCTTCCGAGA GCTGGGAAAT TATAAGAGAA
    GATGAAGCCA CCATGTATAG CAATCAGGCC TTCCCATCAC CTCCAGCATC AAATAACTTG
    GATGTTCAGA CTCATCTTGC AGAAGAATTG AATGCCAGAC TCACAATTTG TAGAAACTCA
    GCCACCAGCC AGCCAGTATC CAGCTCAAAT CATTCCAGCA GAAGAGGCAG CTTACAAGCC
    TATACTTTTG AGGAACAGCC TACACTTCCT GTGCTTTTGC CTACTTCATC TGGATTACCA
    CCAGGTTGGG AGGAAAAACA AGATGAAAGA GGAAGATCAT ATTATGTAGA TCACAATTCC
    AGAACTACTA CCTGGACAAA GCCCGTTGTA CAGGCCACGG TGGAGACCAG TCAGCTGCAG
    CCAAGCCAGA GTGCGGCATG TCCTCAACCA CAGGTCCCCT TGAGTGAGTC AGCGCAGCAG
    GGGACCCAGG CGGCTGAAAT GGAGCAAGGA TTCCTCCCTA AAGGCTGGGA AGTCCGGCAT
    GCGCCAAATG GGAGGCCTTT CTTTATTGAC CACAACACCA AAACCACTAC CTGGGAAGAT
    CCAAGACTGA AAATTCCAGC TCATCTGAGA GGAAAGATGT CACTGGATTC TTCCAATGAT
    CTGGGGCCTT TACCTCCAGG ATGGGAAGAA AGAACTCATA CAGATGGAAG AATCTTCTAC
    ATAAATCACA ATATAAAAAG AACACAATGG GAAGATCCTC GGTTGCAAAA TGTAGCAATA
    ACTGGACCAG CAGTGCCCTA CTCCAGGGAT TACAAAAGAA AGTATGAGTT CTTCCGACGA
    AAGTTGAAGA AGCAGAACGA CATTCCAAAC AAATTTGAAA TGAAACTTCG CCGTGCAACT
    GTTCTTGAGG ACTCTTACAG GAGGATTATG GGTGTCAAGA GAGCGGATTT CCTCAAGGCT
    CGACTGTGGA TTGAGTTCGA TGGTGAAAAG GGATTAGATT ACGGAGGAGT CGCTAGAGAA
    TGGTTTTTCC TGATCTCAAA GGAAATGTTT AACCCTTATT ATGGGTTGTT TGAATATTCT
    GCTACGGATA ATTACACCCT ACAGATAAAT CCAAACTCTG GATTGTGTAA CGAGGATCAC
    CTCTCTTACT TCAAGTTTAT TGGCCGAGTA GCTGGAATGG CAGTTTATCA TGGCAAACTG
    TTGGATGGTT TCTTCATCCG CCCATTTTAC AAGATGATGC TACACAAACC AATAACACTT
    CATGATATGG AATCGGTGGA TGGTGAATAT TACAATTCAC TAAGATGGAT TCTTGAAAAT
    GATCCAACAG AATTGGACCT CAGGTTTGTC ATAGATGAAG AACTTTTTGG ACAGACACAT
    CAACATGAAT TGAAAATTGG TGGATCAGAA ATAGTTGTCA CCAATAAGAA CAAAAAGGAA
    TATATTTATC TTGTAATACA GTGGCGATTT GTAAACCGAA TCCAGAAGCA AATGGCTGCT
    TTTAAAGAGG GATTTTTTGA ACTAATACCA CAGGATCTCA TCAAAATTTT CGATGAAAAT
    GAACTAGAGC TTCTTATGTG TGGATTGGGA GATGTCGATG TGAATGACTG GAGAGAACAC
    ACAAAATATA AGAATGGCTA CAGTGTGAAT CATCAAGTCA TACAGTGGTT CTGGAAGGCT
    GTTCTAATGA TGGATTCAGA AAAAAGAATA AGATTACTTC AGTTTGTCAC TGGTACATCT
    CGAGTGCCTA TGAATGGATT TGCTGAACTC TATGGCTCAA ATGGACCACA GTCATTTACA
    GTTGAACAGT GGGGTACCCC GGAAAAGCTG CCAAGAGCCC ATACATGCTT TAATCGCTTG
    GACTTGCCAC CCTATGAATC ATTTGAAGAA TTATGGGATA AACTGCAGAT GGCAATTGAG
    AACACTCAAG GCTTTGATGG CGTTGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe28897
    Clone ID Related Accession (Same CDS sequence) XM_005638440.2 , XM_022412612.1
    Accession Version XM_022412612.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2550bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase NEDD4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAATGGAG TTTTTACAAG TATGCAAACG AAAACCATTA AAAAGACTTT GAATCCAAAA 
    TGGAATGAAG AAATATTATT CAGAGTCCAT CCTCAGCAGC ACCGGCTTCT TTTTGAAGTA
    TTTGATGAAA ATCGATTGAC CAGAGATGAT TTCCTAGGTC AAGTGGATGT TCCTCTTTAT
    CCATTACCGA CAGAAAATCC AAGAATGGAG AGACCATATA CGTTTAAGGA TTTTGTTCTT
    CACCCAAGAA GTCACAAATC AAGAGTTAAA GGTTATTTGA GATTAAAAAT GACTTATTTA
    CCTAAAACCA GTGGCTCAGA AGAAGATAAC GCAGAACAGG CCGAGGAACT AGAGCCTGGC
    TGGGTTGTTT TGGACCAACC GGATGCTGCC TGCCATTTGC AGCAGCAGCA AGAACCTTCT
    CCTCTACCTC CAGGATGGGA AGAGAGGCAG GATATTCTTG GAAGGACCTA CTATGTAAAC
    CATGAATCTA GAAGAACACA GTGGAAAAGA CCAACCGCTC AGGACAACCT AACTGATGCT
    GAGAATGGTA ACATGCAACT GCAAGCACAG CGTGCATTTA CTACCAGACG GCAGATATCT
    GAGGAAACAG AAAGTGTGGA TAATCGAGAG TCTTCCGAGA GCTGGGAAAT TATAAGAGAA
    GATGAAGCCA CCATGTATAG CAATCAGGCC TTCCCATCAC CTCCAGCATC AAATAACTTG
    GATGTTCAGA CTCATCTTGC AGAAGAATTG AATGCCAGAC TCACAATTTG TAGAAACTCA
    GCCACCAGCC AGCCAGTATC CAGCTCAAAT CATTCCAGCA GAAGAGGCAG CTTACAAGCC
    TATACTTTTG AGGAACAGCC TACACTTCCT GTGCTTTTGC CTACTTCATC TGGATTACCA
    CCAGGTTGGG AGGAAAAACA AGATGAAAGA GGAAGATCAT ATTATGTAGA TCACAATTCC
    AGAACTACTA CCTGGACAAA GCCCGTTGTA CAGGCCACGG TGGAGACCAG TCAGCTGCAG
    CCAAGCCAGA GTGCGGCATG TCCTCAACCA CAGGTCCCCT TGAGTGAGTC AGCGCAGCAG
    GGGACCCAGG CGGCTGAAAT GGAGCAAGGA TTCCTCCCTA AAGGCTGGGA AGTCCGGCAT
    GCGCCAAATG GGAGGCCTTT CTTTATTGAC CACAACACCA AAACCACTAC CTGGGAAGAT
    CCAAGACTGA AAATTCCAGC TCATCTGAGA GGAAAGATGT CACTGGATTC TTCCAATGAT
    CTGGGGCCTT TACCTCCAGG ATGGGAAGAA AGAACTCATA CAGATGGAAG AATCTTCTAC
    ATAAATCACA ATATAAAAAG AACACAATGG GAAGATCCTC GGTTGCAAAA TGTAGCAATA
    ACTGGACCAG CAGTGCCCTA CTCCAGGGAT TACAAAAGAA AGTATGAGTT CTTCCGACGA
    AAGTTGAAGA AGCAGAACGA CATTCCAAAC AAATTTGAAA TGAAACTTCG CCGTGCAACT
    GTTCTTGAGG ACTCTTACAG GAGGATTATG GGTGTCAAGA GAGCGGATTT CCTCAAGGCT
    CGACTGTGGA TTGAGTTCGA TGGTGAAAAG GGATTAGATT ACGGAGGAGT CGCTAGAGAA
    TGGTTTTTCC TGATCTCAAA GGAAATGTTT AACCCTTATT ATGGGTTGTT TGAATATTCT
    GCTACGGATA ATTACACCCT ACAGATAAAT CCAAACTCTG GATTGTGTAA CGAGGATCAC
    CTCTCTTACT TCAAGTTTAT TGGCCGAGTA GCTGGAATGG CAGTTTATCA TGGCAAACTG
    TTGGATGGTT TCTTCATCCG CCCATTTTAC AAGATGATGC TACACAAACC AATAACACTT
    CATGATATGG AATCGGTGGA TGGTGAATAT TACAATTCAC TAAGATGGAT TCTTGAAAAT
    GATCCAACAG AATTGGACCT CAGGTTTGTC ATAGATGAAG AACTTTTTGG ACAGACACAT
    CAACATGAAT TGAAAATTGG TGGATCAGAA ATAGTTGTCA CCAATAAGAA CAAAAAGGAA
    TATATTTATC TTGTAATACA GTGGCGATTT GTAAACCGAA TCCAGAAGCA AATGGCTGCT
    TTTAAAGAGG GATTTTTTGA ACTAATACCA CAGGATCTCA TCAAAATTTT CGATGAAAAT
    GAACTAGAGC TTCTTATGTG TGGATTGGGA GATGTCGATG TGAATGACTG GAGAGAACAC
    ACAAAATATA AGAATGGCTA CAGTGTGAAT CATCAAGTCA TACAGTGGTT CTGGAAGGCT
    GTTCTAATGA TGGATTCAGA AAAAAGAATA AGATTACTTC AGTTTGTCAC TGGTACATCT
    CGAGTGCCTA TGAATGGATT TGCTGAACTC TATGGCTCAA ATGGACCACA GTCATTTACA
    GTTGAACAGT GGGGTACCCC GGAAAAGCTG CCAAGAGCCC ATACATGCTT TAATCGCTTG
    GACTTGCCAC CCTATGAATC ATTTGAAGAA TTATGGGATA AACTGCAGAT GGCAATTGAG
    AACACTCAAG GCTTTGATGG CGTTGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268320.1
    CDS146..2695
    Translation

    Target ORF information:

    RefSeq Version XM_022412612.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022412612.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAATGGAG TTTTTACAAG TATGCAAACG AAAACCATTA AAAAGACTTT GAATCCAAAA 
    TGGAATGAAG AAATATTATT CAGAGTCCAT CCTCAGCAGC ACCGGCTTCT TTTTGAAGTA
    TTTGATGAAA ATCGATTGAC CAGAGATGAT TTCCTAGGTC AAGTGGATGT TCCTCTTTAT
    CCATTACCGA CAGAAAATCC AAGAATGGAG AGACCATATA CGTTTAAGGA TTTTGTTCTT
    CACCCAAGAA GTCACAAATC AAGAGTTAAA GGTTATTTGA GATTAAAAAT GACTTATTTA
    CCTAAAACCA GTGGCTCAGA AGAAGATAAC GCAGAACAGG CCGAGGAACT AGAGCCTGGC
    TGGGTTGTTT TGGACCAACC GGATGCTGCC TGCCATTTGC AGCAGCAGCA AGAACCTTCT
    CCTCTACCTC CAGGATGGGA AGAGAGGCAG GATATTCTTG GAAGGACCTA CTATGTAAAC
    CATGAATCTA GAAGAACACA GTGGAAAAGA CCAACCGCTC AGGACAACCT AACTGATGCT
    GAGAATGGTA ACATGCAACT GCAAGCACAG CGTGCATTTA CTACCAGACG GCAGATATCT
    GAGGAAACAG AAAGTGTGGA TAATCGAGAG TCTTCCGAGA GCTGGGAAAT TATAAGAGAA
    GATGAAGCCA CCATGTATAG CAATCAGGCC TTCCCATCAC CTCCAGCATC AAATAACTTG
    GATGTTCAGA CTCATCTTGC AGAAGAATTG AATGCCAGAC TCACAATTTG TAGAAACTCA
    GCCACCAGCC AGCCAGTATC CAGCTCAAAT CATTCCAGCA GAAGAGGCAG CTTACAAGCC
    TATACTTTTG AGGAACAGCC TACACTTCCT GTGCTTTTGC CTACTTCATC TGGATTACCA
    CCAGGTTGGG AGGAAAAACA AGATGAAAGA GGAAGATCAT ATTATGTAGA TCACAATTCC
    AGAACTACTA CCTGGACAAA GCCCGTTGTA CAGGCCACGG TGGAGACCAG TCAGCTGCAG
    CCAAGCCAGA GTGCGGCATG TCCTCAACCA CAGGTCCCCT TGAGTGAGTC AGCGCAGCAG
    GGGACCCAGG CGGCTGAAAT GGAGCAAGGA TTCCTCCCTA AAGGCTGGGA AGTCCGGCAT
    GCGCCAAATG GGAGGCCTTT CTTTATTGAC CACAACACCA AAACCACTAC CTGGGAAGAT
    CCAAGACTGA AAATTCCAGC TCATCTGAGA GGAAAGATGT CACTGGATTC TTCCAATGAT
    CTGGGGCCTT TACCTCCAGG ATGGGAAGAA AGAACTCATA CAGATGGAAG AATCTTCTAC
    ATAAATCACA ATATAAAAAG AACACAATGG GAAGATCCTC GGTTGCAAAA TGTAGCAATA
    ACTGGACCAG CAGTGCCCTA CTCCAGGGAT TACAAAAGAA AGTATGAGTT CTTCCGACGA
    AAGTTGAAGA AGCAGAACGA CATTCCAAAC AAATTTGAAA TGAAACTTCG CCGTGCAACT
    GTTCTTGAGG ACTCTTACAG GAGGATTATG GGTGTCAAGA GAGCGGATTT CCTCAAGGCT
    CGACTGTGGA TTGAGTTCGA TGGTGAAAAG GGATTAGATT ACGGAGGAGT CGCTAGAGAA
    TGGTTTTTCC TGATCTCAAA GGAAATGTTT AACCCTTATT ATGGGTTGTT TGAATATTCT
    GCTACGGATA ATTACACCCT ACAGATAAAT CCAAACTCTG GATTGTGTAA CGAGGATCAC
    CTCTCTTACT TCAAGTTTAT TGGCCGAGTA GCTGGAATGG CAGTTTATCA TGGCAAACTG
    TTGGATGGTT TCTTCATCCG CCCATTTTAC AAGATGATGC TACACAAACC AATAACACTT
    CATGATATGG AATCGGTGGA TGGTGAATAT TACAATTCAC TAAGATGGAT TCTTGAAAAT
    GATCCAACAG AATTGGACCT CAGGTTTGTC ATAGATGAAG AACTTTTTGG ACAGACACAT
    CAACATGAAT TGAAAATTGG TGGATCAGAA ATAGTTGTCA CCAATAAGAA CAAAAAGGAA
    TATATTTATC TTGTAATACA GTGGCGATTT GTAAACCGAA TCCAGAAGCA AATGGCTGCT
    TTTAAAGAGG GATTTTTTGA ACTAATACCA CAGGATCTCA TCAAAATTTT CGATGAAAAT
    GAACTAGAGC TTCTTATGTG TGGATTGGGA GATGTCGATG TGAATGACTG GAGAGAACAC
    ACAAAATATA AGAATGGCTA CAGTGTGAAT CATCAAGTCA TACAGTGGTT CTGGAAGGCT
    GTTCTAATGA TGGATTCAGA AAAAAGAATA AGATTACTTC AGTTTGTCAC TGGTACATCT
    CGAGTGCCTA TGAATGGATT TGCTGAACTC TATGGCTCAA ATGGACCACA GTCATTTACA
    GTTGAACAGT GGGGTACCCC GGAAAAGCTG CCAAGAGCCC ATACATGCTT TAATCGCTTG
    GACTTGCCAC CCTATGAATC ATTTGAAGAA TTATGGGATA AACTGCAGAT GGCAATTGAG
    AACACTCAAG GCTTTGATGG CGTTGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe28898
    Clone ID Related Accession (Same CDS sequence) XM_005638441.3 , XM_022412613.1 , XM_022412614.1 , XM_005638441.2
    Accession Version XM_005638441.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2346bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase NEDD4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005638441.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGAGAGAC CATATACGTT TAAGGATTTT GTTCTTCACC CAAGAAGTCA CAAATCAAGA 
    GTTAAAGGTT ATTTGAGATT AAAAATGACT TATTTACCTA AAACCAGTGG CTCAGAAGAA
    GATAACGCAG AACAGGCCGA GGAACTAGAG CCTGGCTGGG TTGTTTTGGA CCAACCGGAT
    GCTGCCTGCC ATTTGCAGCA GCAGCAAGAA CCTTCTCCTC TACCTCCAGG ATGGGAAGAG
    AGGCAGGATA TTCTTGGAAG GACCTACTAT GTAAACCATG AATCTAGAAG AACACAGTGG
    AAAAGACCAA CCGCTCAGGA CAACCTAACT GATGCTGAGA ATGGTAACAT GCAACTGCAA
    GCACAGCGTG CATTTACTAC CAGACGGCAG ATATCTGAGG AAACAGAAAG TGTGGATAAT
    CGAGAGTCTT CCGAGAGCTG GGAAATTATA AGAGAAGATG AAGCCACCAT GTATAGCAAT
    CAGGCCTTCC CATCACCTCC AGCATCAAAT AACTTGGATG TTCAGACTCA TCTTGCAGAA
    GAATTGAATG CCAGACTCAC AATTTGTAGA AACTCAGCCA CCAGCCAGCC AGTATCCAGC
    TCAAATCATT CCAGCAGAAG AGGCAGCTTA CAAGCCTATA CTTTTGAGGA ACAGCCTACA
    CTTCCTGTGC TTTTGCCTAC TTCATCTGGA TTACCACCAG GTTGGGAGGA AAAACAAGAT
    GAAAGAGGAA GATCATATTA TGTAGATCAC AATTCCAGAA CTACTACCTG GACAAAGCCC
    GTTGTACAGG CCACGGTGGA GACCAGTCAG CTGCAGCCAA GCCAGAGTGC GGCATGTCCT
    CAACCACAGG TCCCCTTGAG TGAGTCAGCG CAGCAGGGGA CCCAGGCGGC TGAAATGGAG
    CAAGGATTCC TCCCTAAAGG CTGGGAAGTC CGGCATGCGC CAAATGGGAG GCCTTTCTTT
    ATTGACCACA ACACCAAAAC CACTACCTGG GAAGATCCAA GACTGAAAAT TCCAGCTCAT
    CTGAGAGGAA AGATGTCACT GGATTCTTCC AATGATCTGG GGCCTTTACC TCCAGGATGG
    GAAGAAAGAA CTCATACAGA TGGAAGAATC TTCTACATAA ATCACAATAT AAAAAGAACA
    CAATGGGAAG ATCCTCGGTT GCAAAATGTA GCAATAACTG GACCAGCAGT GCCCTACTCC
    AGGGATTACA AAAGAAAGTA TGAGTTCTTC CGACGAAAGT TGAAGAAGCA GAACGACATT
    CCAAACAAAT TTGAAATGAA ACTTCGCCGT GCAACTGTTC TTGAGGACTC TTACAGGAGG
    ATTATGGGTG TCAAGAGAGC GGATTTCCTC AAGGCTCGAC TGTGGATTGA GTTCGATGGT
    GAAAAGGGAT TAGATTACGG AGGAGTCGCT AGAGAATGGT TTTTCCTGAT CTCAAAGGAA
    ATGTTTAACC CTTATTATGG GTTGTTTGAA TATTCTGCTA CGGATAATTA CACCCTACAG
    ATAAATCCAA ACTCTGGATT GTGTAACGAG GATCACCTCT CTTACTTCAA GTTTATTGGC
    CGAGTAGCTG GAATGGCAGT TTATCATGGC AAACTGTTGG ATGGTTTCTT CATCCGCCCA
    TTTTACAAGA TGATGCTACA CAAACCAATA ACACTTCATG ATATGGAATC GGTGGATGGT
    GAATATTACA ATTCACTAAG ATGGATTCTT GAAAATGATC CAACAGAATT GGACCTCAGG
    TTTGTCATAG ATGAAGAACT TTTTGGACAG ACACATCAAC ATGAATTGAA AATTGGTGGA
    TCAGAAATAG TTGTCACCAA TAAGAACAAA AAGGAATATA TTTATCTTGT AATACAGTGG
    CGATTTGTAA ACCGAATCCA GAAGCAAATG GCTGCTTTTA AAGAGGGATT TTTTGAACTA
    ATACCACAGG ATCTCATCAA AATTTTCGAT GAAAATGAAC TAGAGCTTCT TATGTGTGGA
    TTGGGAGATG TCGATGTGAA TGACTGGAGA GAACACACAA AATATAAGAA TGGCTACAGT
    GTGAATCATC AAGTCATACA GTGGTTCTGG AAGGCTGTTC TAATGATGGA TTCAGAAAAA
    AGAATAAGAT TACTTCAGTT TGTCACTGGT ACATCTCGAG TGCCTATGAA TGGATTTGCT
    GAACTCTATG GCTCAAATGG ACCACAGTCA TTTACAGTTG AACAGTGGGG TACCCCGGAA
    AAGCTGCCAA GAGCCCATAC ATGCTTTAAT CGCTTGGACT TGCCACCCTA TGAATCATTT
    GAAGAATTAT GGGATAAACT GCAGATGGCA ATTGAGAACA CTCAAGGCTT TGATGGCGTT
    GATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005638498.1
    CDS202..2547
    Translation

    Target ORF information:

    RefSeq Version XM_005638441.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005638441.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGAGAGAC CATATACGTT TAAGGATTTT GTTCTTCACC CAAGAAGTCA CAAATCAAGA 
    GTTAAAGGTT ATTTGAGATT AAAAATGACT TATTTACCTA AAACCAGTGG CTCAGAAGAA
    GATAACGCAG AACAGGCCGA GGAACTAGAG CCTGGCTGGG TTGTTTTGGA CCAACCGGAT
    GCTGCCTGCC ATTTGCAGCA GCAGCAAGAA CCTTCTCCTC TACCTCCAGG ATGGGAAGAG
    AGGCAGGATA TTCTTGGAAG GACCTACTAT GTAAACCATG AATCTAGAAG AACACAGTGG
    AAAAGACCAA CCGCTCAGGA CAACCTAACT GATGCTGAGA ATGGTAACAT GCAACTGCAA
    GCACAGCGTG CATTTACTAC CAGACGGCAG ATATCTGAGG AAACAGAAAG TGTGGATAAT
    CGAGAGTCTT CCGAGAGCTG GGAAATTATA AGAGAAGATG AAGCCACCAT GTATAGCAAT
    CAGGCCTTCC CATCACCTCC AGCATCAAAT AACTTGGATG TTCAGACTCA TCTTGCAGAA
    GAATTGAATG CCAGACTCAC AATTTGTAGA AACTCAGCCA CCAGCCAGCC AGTATCCAGC
    TCAAATCATT CCAGCAGAAG AGGCAGCTTA CAAGCCTATA CTTTTGAGGA ACAGCCTACA
    CTTCCTGTGC TTTTGCCTAC TTCATCTGGA TTACCACCAG GTTGGGAGGA AAAACAAGAT
    GAAAGAGGAA GATCATATTA TGTAGATCAC AATTCCAGAA CTACTACCTG GACAAAGCCC
    GTTGTACAGG CCACGGTGGA GACCAGTCAG CTGCAGCCAA GCCAGAGTGC GGCATGTCCT
    CAACCACAGG TCCCCTTGAG TGAGTCAGCG CAGCAGGGGA CCCAGGCGGC TGAAATGGAG
    CAAGGATTCC TCCCTAAAGG CTGGGAAGTC CGGCATGCGC CAAATGGGAG GCCTTTCTTT
    ATTGACCACA ACACCAAAAC CACTACCTGG GAAGATCCAA GACTGAAAAT TCCAGCTCAT
    CTGAGAGGAA AGATGTCACT GGATTCTTCC AATGATCTGG GGCCTTTACC TCCAGGATGG
    GAAGAAAGAA CTCATACAGA TGGAAGAATC TTCTACATAA ATCACAATAT AAAAAGAACA
    CAATGGGAAG ATCCTCGGTT GCAAAATGTA GCAATAACTG GACCAGCAGT GCCCTACTCC
    AGGGATTACA AAAGAAAGTA TGAGTTCTTC CGACGAAAGT TGAAGAAGCA GAACGACATT
    CCAAACAAAT TTGAAATGAA ACTTCGCCGT GCAACTGTTC TTGAGGACTC TTACAGGAGG
    ATTATGGGTG TCAAGAGAGC GGATTTCCTC AAGGCTCGAC TGTGGATTGA GTTCGATGGT
    GAAAAGGGAT TAGATTACGG AGGAGTCGCT AGAGAATGGT TTTTCCTGAT CTCAAAGGAA
    ATGTTTAACC CTTATTATGG GTTGTTTGAA TATTCTGCTA CGGATAATTA CACCCTACAG
    ATAAATCCAA ACTCTGGATT GTGTAACGAG GATCACCTCT CTTACTTCAA GTTTATTGGC
    CGAGTAGCTG GAATGGCAGT TTATCATGGC AAACTGTTGG ATGGTTTCTT CATCCGCCCA
    TTTTACAAGA TGATGCTACA CAAACCAATA ACACTTCATG ATATGGAATC GGTGGATGGT
    GAATATTACA ATTCACTAAG ATGGATTCTT GAAAATGATC CAACAGAATT GGACCTCAGG
    TTTGTCATAG ATGAAGAACT TTTTGGACAG ACACATCAAC ATGAATTGAA AATTGGTGGA
    TCAGAAATAG TTGTCACCAA TAAGAACAAA AAGGAATATA TTTATCTTGT AATACAGTGG
    CGATTTGTAA ACCGAATCCA GAAGCAAATG GCTGCTTTTA AAGAGGGATT TTTTGAACTA
    ATACCACAGG ATCTCATCAA AATTTTCGAT GAAAATGAAC TAGAGCTTCT TATGTGTGGA
    TTGGGAGATG TCGATGTGAA TGACTGGAGA GAACACACAA AATATAAGAA TGGCTACAGT
    GTGAATCATC AAGTCATACA GTGGTTCTGG AAGGCTGTTC TAATGATGGA TTCAGAAAAA
    AGAATAAGAT TACTTCAGTT TGTCACTGGT ACATCTCGAG TGCCTATGAA TGGATTTGCT
    GAACTCTATG GCTCAAATGG ACCACAGTCA TTTACAGTTG AACAGTGGGG TACCCCGGAA
    AAGCTGCCAA GAGCCCATAC ATGCTTTAAT CGCTTGGACT TGCCACCCTA TGAATCATTT
    GAAGAATTAT GGGATAAACT GCAGATGGCA ATTGAGAACA CTCAAGGCTT TGATGGCGTT
    GATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe28898
    Clone ID Related Accession (Same CDS sequence) XM_005638441.3 , XM_022412613.1 , XM_022412614.1 , XM_005638441.2
    Accession Version XM_022412613.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2346bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase NEDD4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGAGAGAC CATATACGTT TAAGGATTTT GTTCTTCACC CAAGAAGTCA CAAATCAAGA 
    GTTAAAGGTT ATTTGAGATT AAAAATGACT TATTTACCTA AAACCAGTGG CTCAGAAGAA
    GATAACGCAG AACAGGCCGA GGAACTAGAG CCTGGCTGGG TTGTTTTGGA CCAACCGGAT
    GCTGCCTGCC ATTTGCAGCA GCAGCAAGAA CCTTCTCCTC TACCTCCAGG ATGGGAAGAG
    AGGCAGGATA TTCTTGGAAG GACCTACTAT GTAAACCATG AATCTAGAAG AACACAGTGG
    AAAAGACCAA CCGCTCAGGA CAACCTAACT GATGCTGAGA ATGGTAACAT GCAACTGCAA
    GCACAGCGTG CATTTACTAC CAGACGGCAG ATATCTGAGG AAACAGAAAG TGTGGATAAT
    CGAGAGTCTT CCGAGAGCTG GGAAATTATA AGAGAAGATG AAGCCACCAT GTATAGCAAT
    CAGGCCTTCC CATCACCTCC AGCATCAAAT AACTTGGATG TTCAGACTCA TCTTGCAGAA
    GAATTGAATG CCAGACTCAC AATTTGTAGA AACTCAGCCA CCAGCCAGCC AGTATCCAGC
    TCAAATCATT CCAGCAGAAG AGGCAGCTTA CAAGCCTATA CTTTTGAGGA ACAGCCTACA
    CTTCCTGTGC TTTTGCCTAC TTCATCTGGA TTACCACCAG GTTGGGAGGA AAAACAAGAT
    GAAAGAGGAA GATCATATTA TGTAGATCAC AATTCCAGAA CTACTACCTG GACAAAGCCC
    GTTGTACAGG CCACGGTGGA GACCAGTCAG CTGCAGCCAA GCCAGAGTGC GGCATGTCCT
    CAACCACAGG TCCCCTTGAG TGAGTCAGCG CAGCAGGGGA CCCAGGCGGC TGAAATGGAG
    CAAGGATTCC TCCCTAAAGG CTGGGAAGTC CGGCATGCGC CAAATGGGAG GCCTTTCTTT
    ATTGACCACA ACACCAAAAC CACTACCTGG GAAGATCCAA GACTGAAAAT TCCAGCTCAT
    CTGAGAGGAA AGATGTCACT GGATTCTTCC AATGATCTGG GGCCTTTACC TCCAGGATGG
    GAAGAAAGAA CTCATACAGA TGGAAGAATC TTCTACATAA ATCACAATAT AAAAAGAACA
    CAATGGGAAG ATCCTCGGTT GCAAAATGTA GCAATAACTG GACCAGCAGT GCCCTACTCC
    AGGGATTACA AAAGAAAGTA TGAGTTCTTC CGACGAAAGT TGAAGAAGCA GAACGACATT
    CCAAACAAAT TTGAAATGAA ACTTCGCCGT GCAACTGTTC TTGAGGACTC TTACAGGAGG
    ATTATGGGTG TCAAGAGAGC GGATTTCCTC AAGGCTCGAC TGTGGATTGA GTTCGATGGT
    GAAAAGGGAT TAGATTACGG AGGAGTCGCT AGAGAATGGT TTTTCCTGAT CTCAAAGGAA
    ATGTTTAACC CTTATTATGG GTTGTTTGAA TATTCTGCTA CGGATAATTA CACCCTACAG
    ATAAATCCAA ACTCTGGATT GTGTAACGAG GATCACCTCT CTTACTTCAA GTTTATTGGC
    CGAGTAGCTG GAATGGCAGT TTATCATGGC AAACTGTTGG ATGGTTTCTT CATCCGCCCA
    TTTTACAAGA TGATGCTACA CAAACCAATA ACACTTCATG ATATGGAATC GGTGGATGGT
    GAATATTACA ATTCACTAAG ATGGATTCTT GAAAATGATC CAACAGAATT GGACCTCAGG
    TTTGTCATAG ATGAAGAACT TTTTGGACAG ACACATCAAC ATGAATTGAA AATTGGTGGA
    TCAGAAATAG TTGTCACCAA TAAGAACAAA AAGGAATATA TTTATCTTGT AATACAGTGG
    CGATTTGTAA ACCGAATCCA GAAGCAAATG GCTGCTTTTA AAGAGGGATT TTTTGAACTA
    ATACCACAGG ATCTCATCAA AATTTTCGAT GAAAATGAAC TAGAGCTTCT TATGTGTGGA
    TTGGGAGATG TCGATGTGAA TGACTGGAGA GAACACACAA AATATAAGAA TGGCTACAGT
    GTGAATCATC AAGTCATACA GTGGTTCTGG AAGGCTGTTC TAATGATGGA TTCAGAAAAA
    AGAATAAGAT TACTTCAGTT TGTCACTGGT ACATCTCGAG TGCCTATGAA TGGATTTGCT
    GAACTCTATG GCTCAAATGG ACCACAGTCA TTTACAGTTG AACAGTGGGG TACCCCGGAA
    AAGCTGCCAA GAGCCCATAC ATGCTTTAAT CGCTTGGACT TGCCACCCTA TGAATCATTT
    GAAGAATTAT GGGATAAACT GCAGATGGCA ATTGAGAACA CTCAAGGCTT TGATGGCGTT
    GATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268321.1
    CDS236..2581
    Translation

    Target ORF information:

    RefSeq Version XM_022412613.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022412613.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGAGAGAC CATATACGTT TAAGGATTTT GTTCTTCACC CAAGAAGTCA CAAATCAAGA 
    GTTAAAGGTT ATTTGAGATT AAAAATGACT TATTTACCTA AAACCAGTGG CTCAGAAGAA
    GATAACGCAG AACAGGCCGA GGAACTAGAG CCTGGCTGGG TTGTTTTGGA CCAACCGGAT
    GCTGCCTGCC ATTTGCAGCA GCAGCAAGAA CCTTCTCCTC TACCTCCAGG ATGGGAAGAG
    AGGCAGGATA TTCTTGGAAG GACCTACTAT GTAAACCATG AATCTAGAAG AACACAGTGG
    AAAAGACCAA CCGCTCAGGA CAACCTAACT GATGCTGAGA ATGGTAACAT GCAACTGCAA
    GCACAGCGTG CATTTACTAC CAGACGGCAG ATATCTGAGG AAACAGAAAG TGTGGATAAT
    CGAGAGTCTT CCGAGAGCTG GGAAATTATA AGAGAAGATG AAGCCACCAT GTATAGCAAT
    CAGGCCTTCC CATCACCTCC AGCATCAAAT AACTTGGATG TTCAGACTCA TCTTGCAGAA
    GAATTGAATG CCAGACTCAC AATTTGTAGA AACTCAGCCA CCAGCCAGCC AGTATCCAGC
    TCAAATCATT CCAGCAGAAG AGGCAGCTTA CAAGCCTATA CTTTTGAGGA ACAGCCTACA
    CTTCCTGTGC TTTTGCCTAC TTCATCTGGA TTACCACCAG GTTGGGAGGA AAAACAAGAT
    GAAAGAGGAA GATCATATTA TGTAGATCAC AATTCCAGAA CTACTACCTG GACAAAGCCC
    GTTGTACAGG CCACGGTGGA GACCAGTCAG CTGCAGCCAA GCCAGAGTGC GGCATGTCCT
    CAACCACAGG TCCCCTTGAG TGAGTCAGCG CAGCAGGGGA CCCAGGCGGC TGAAATGGAG
    CAAGGATTCC TCCCTAAAGG CTGGGAAGTC CGGCATGCGC CAAATGGGAG GCCTTTCTTT
    ATTGACCACA ACACCAAAAC CACTACCTGG GAAGATCCAA GACTGAAAAT TCCAGCTCAT
    CTGAGAGGAA AGATGTCACT GGATTCTTCC AATGATCTGG GGCCTTTACC TCCAGGATGG
    GAAGAAAGAA CTCATACAGA TGGAAGAATC TTCTACATAA ATCACAATAT AAAAAGAACA
    CAATGGGAAG ATCCTCGGTT GCAAAATGTA GCAATAACTG GACCAGCAGT GCCCTACTCC
    AGGGATTACA AAAGAAAGTA TGAGTTCTTC CGACGAAAGT TGAAGAAGCA GAACGACATT
    CCAAACAAAT TTGAAATGAA ACTTCGCCGT GCAACTGTTC TTGAGGACTC TTACAGGAGG
    ATTATGGGTG TCAAGAGAGC GGATTTCCTC AAGGCTCGAC TGTGGATTGA GTTCGATGGT
    GAAAAGGGAT TAGATTACGG AGGAGTCGCT AGAGAATGGT TTTTCCTGAT CTCAAAGGAA
    ATGTTTAACC CTTATTATGG GTTGTTTGAA TATTCTGCTA CGGATAATTA CACCCTACAG
    ATAAATCCAA ACTCTGGATT GTGTAACGAG GATCACCTCT CTTACTTCAA GTTTATTGGC
    CGAGTAGCTG GAATGGCAGT TTATCATGGC AAACTGTTGG ATGGTTTCTT CATCCGCCCA
    TTTTACAAGA TGATGCTACA CAAACCAATA ACACTTCATG ATATGGAATC GGTGGATGGT
    GAATATTACA ATTCACTAAG ATGGATTCTT GAAAATGATC CAACAGAATT GGACCTCAGG
    TTTGTCATAG ATGAAGAACT TTTTGGACAG ACACATCAAC ATGAATTGAA AATTGGTGGA
    TCAGAAATAG TTGTCACCAA TAAGAACAAA AAGGAATATA TTTATCTTGT AATACAGTGG
    CGATTTGTAA ACCGAATCCA GAAGCAAATG GCTGCTTTTA AAGAGGGATT TTTTGAACTA
    ATACCACAGG ATCTCATCAA AATTTTCGAT GAAAATGAAC TAGAGCTTCT TATGTGTGGA
    TTGGGAGATG TCGATGTGAA TGACTGGAGA GAACACACAA AATATAAGAA TGGCTACAGT
    GTGAATCATC AAGTCATACA GTGGTTCTGG AAGGCTGTTC TAATGATGGA TTCAGAAAAA
    AGAATAAGAT TACTTCAGTT TGTCACTGGT ACATCTCGAG TGCCTATGAA TGGATTTGCT
    GAACTCTATG GCTCAAATGG ACCACAGTCA TTTACAGTTG AACAGTGGGG TACCCCGGAA
    AAGCTGCCAA GAGCCCATAC ATGCTTTAAT CGCTTGGACT TGCCACCCTA TGAATCATTT
    GAAGAATTAT GGGATAAACT GCAGATGGCA ATTGAGAACA CTCAAGGCTT TGATGGCGTT
    GATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe28898
    Clone ID Related Accession (Same CDS sequence) XM_005638441.3 , XM_022412613.1 , XM_022412614.1 , XM_005638441.2
    Accession Version XM_022412614.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2346bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase NEDD4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGAGAGAC CATATACGTT TAAGGATTTT GTTCTTCACC CAAGAAGTCA CAAATCAAGA 
    GTTAAAGGTT ATTTGAGATT AAAAATGACT TATTTACCTA AAACCAGTGG CTCAGAAGAA
    GATAACGCAG AACAGGCCGA GGAACTAGAG CCTGGCTGGG TTGTTTTGGA CCAACCGGAT
    GCTGCCTGCC ATTTGCAGCA GCAGCAAGAA CCTTCTCCTC TACCTCCAGG ATGGGAAGAG
    AGGCAGGATA TTCTTGGAAG GACCTACTAT GTAAACCATG AATCTAGAAG AACACAGTGG
    AAAAGACCAA CCGCTCAGGA CAACCTAACT GATGCTGAGA ATGGTAACAT GCAACTGCAA
    GCACAGCGTG CATTTACTAC CAGACGGCAG ATATCTGAGG AAACAGAAAG TGTGGATAAT
    CGAGAGTCTT CCGAGAGCTG GGAAATTATA AGAGAAGATG AAGCCACCAT GTATAGCAAT
    CAGGCCTTCC CATCACCTCC AGCATCAAAT AACTTGGATG TTCAGACTCA TCTTGCAGAA
    GAATTGAATG CCAGACTCAC AATTTGTAGA AACTCAGCCA CCAGCCAGCC AGTATCCAGC
    TCAAATCATT CCAGCAGAAG AGGCAGCTTA CAAGCCTATA CTTTTGAGGA ACAGCCTACA
    CTTCCTGTGC TTTTGCCTAC TTCATCTGGA TTACCACCAG GTTGGGAGGA AAAACAAGAT
    GAAAGAGGAA GATCATATTA TGTAGATCAC AATTCCAGAA CTACTACCTG GACAAAGCCC
    GTTGTACAGG CCACGGTGGA GACCAGTCAG CTGCAGCCAA GCCAGAGTGC GGCATGTCCT
    CAACCACAGG TCCCCTTGAG TGAGTCAGCG CAGCAGGGGA CCCAGGCGGC TGAAATGGAG
    CAAGGATTCC TCCCTAAAGG CTGGGAAGTC CGGCATGCGC CAAATGGGAG GCCTTTCTTT
    ATTGACCACA ACACCAAAAC CACTACCTGG GAAGATCCAA GACTGAAAAT TCCAGCTCAT
    CTGAGAGGAA AGATGTCACT GGATTCTTCC AATGATCTGG GGCCTTTACC TCCAGGATGG
    GAAGAAAGAA CTCATACAGA TGGAAGAATC TTCTACATAA ATCACAATAT AAAAAGAACA
    CAATGGGAAG ATCCTCGGTT GCAAAATGTA GCAATAACTG GACCAGCAGT GCCCTACTCC
    AGGGATTACA AAAGAAAGTA TGAGTTCTTC CGACGAAAGT TGAAGAAGCA GAACGACATT
    CCAAACAAAT TTGAAATGAA ACTTCGCCGT GCAACTGTTC TTGAGGACTC TTACAGGAGG
    ATTATGGGTG TCAAGAGAGC GGATTTCCTC AAGGCTCGAC TGTGGATTGA GTTCGATGGT
    GAAAAGGGAT TAGATTACGG AGGAGTCGCT AGAGAATGGT TTTTCCTGAT CTCAAAGGAA
    ATGTTTAACC CTTATTATGG GTTGTTTGAA TATTCTGCTA CGGATAATTA CACCCTACAG
    ATAAATCCAA ACTCTGGATT GTGTAACGAG GATCACCTCT CTTACTTCAA GTTTATTGGC
    CGAGTAGCTG GAATGGCAGT TTATCATGGC AAACTGTTGG ATGGTTTCTT CATCCGCCCA
    TTTTACAAGA TGATGCTACA CAAACCAATA ACACTTCATG ATATGGAATC GGTGGATGGT
    GAATATTACA ATTCACTAAG ATGGATTCTT GAAAATGATC CAACAGAATT GGACCTCAGG
    TTTGTCATAG ATGAAGAACT TTTTGGACAG ACACATCAAC ATGAATTGAA AATTGGTGGA
    TCAGAAATAG TTGTCACCAA TAAGAACAAA AAGGAATATA TTTATCTTGT AATACAGTGG
    CGATTTGTAA ACCGAATCCA GAAGCAAATG GCTGCTTTTA AAGAGGGATT TTTTGAACTA
    ATACCACAGG ATCTCATCAA AATTTTCGAT GAAAATGAAC TAGAGCTTCT TATGTGTGGA
    TTGGGAGATG TCGATGTGAA TGACTGGAGA GAACACACAA AATATAAGAA TGGCTACAGT
    GTGAATCATC AAGTCATACA GTGGTTCTGG AAGGCTGTTC TAATGATGGA TTCAGAAAAA
    AGAATAAGAT TACTTCAGTT TGTCACTGGT ACATCTCGAG TGCCTATGAA TGGATTTGCT
    GAACTCTATG GCTCAAATGG ACCACAGTCA TTTACAGTTG AACAGTGGGG TACCCCGGAA
    AAGCTGCCAA GAGCCCATAC ATGCTTTAAT CGCTTGGACT TGCCACCCTA TGAATCATTT
    GAAGAATTAT GGGATAAACT GCAGATGGCA ATTGAGAACA CTCAAGGCTT TGATGGCGTT
    GATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268322.1
    CDS146..2491
    Translation

    Target ORF information:

    RefSeq Version XM_022412614.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022412614.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGAGAGAC CATATACGTT TAAGGATTTT GTTCTTCACC CAAGAAGTCA CAAATCAAGA 
    GTTAAAGGTT ATTTGAGATT AAAAATGACT TATTTACCTA AAACCAGTGG CTCAGAAGAA
    GATAACGCAG AACAGGCCGA GGAACTAGAG CCTGGCTGGG TTGTTTTGGA CCAACCGGAT
    GCTGCCTGCC ATTTGCAGCA GCAGCAAGAA CCTTCTCCTC TACCTCCAGG ATGGGAAGAG
    AGGCAGGATA TTCTTGGAAG GACCTACTAT GTAAACCATG AATCTAGAAG AACACAGTGG
    AAAAGACCAA CCGCTCAGGA CAACCTAACT GATGCTGAGA ATGGTAACAT GCAACTGCAA
    GCACAGCGTG CATTTACTAC CAGACGGCAG ATATCTGAGG AAACAGAAAG TGTGGATAAT
    CGAGAGTCTT CCGAGAGCTG GGAAATTATA AGAGAAGATG AAGCCACCAT GTATAGCAAT
    CAGGCCTTCC CATCACCTCC AGCATCAAAT AACTTGGATG TTCAGACTCA TCTTGCAGAA
    GAATTGAATG CCAGACTCAC AATTTGTAGA AACTCAGCCA CCAGCCAGCC AGTATCCAGC
    TCAAATCATT CCAGCAGAAG AGGCAGCTTA CAAGCCTATA CTTTTGAGGA ACAGCCTACA
    CTTCCTGTGC TTTTGCCTAC TTCATCTGGA TTACCACCAG GTTGGGAGGA AAAACAAGAT
    GAAAGAGGAA GATCATATTA TGTAGATCAC AATTCCAGAA CTACTACCTG GACAAAGCCC
    GTTGTACAGG CCACGGTGGA GACCAGTCAG CTGCAGCCAA GCCAGAGTGC GGCATGTCCT
    CAACCACAGG TCCCCTTGAG TGAGTCAGCG CAGCAGGGGA CCCAGGCGGC TGAAATGGAG
    CAAGGATTCC TCCCTAAAGG CTGGGAAGTC CGGCATGCGC CAAATGGGAG GCCTTTCTTT
    ATTGACCACA ACACCAAAAC CACTACCTGG GAAGATCCAA GACTGAAAAT TCCAGCTCAT
    CTGAGAGGAA AGATGTCACT GGATTCTTCC AATGATCTGG GGCCTTTACC TCCAGGATGG
    GAAGAAAGAA CTCATACAGA TGGAAGAATC TTCTACATAA ATCACAATAT AAAAAGAACA
    CAATGGGAAG ATCCTCGGTT GCAAAATGTA GCAATAACTG GACCAGCAGT GCCCTACTCC
    AGGGATTACA AAAGAAAGTA TGAGTTCTTC CGACGAAAGT TGAAGAAGCA GAACGACATT
    CCAAACAAAT TTGAAATGAA ACTTCGCCGT GCAACTGTTC TTGAGGACTC TTACAGGAGG
    ATTATGGGTG TCAAGAGAGC GGATTTCCTC AAGGCTCGAC TGTGGATTGA GTTCGATGGT
    GAAAAGGGAT TAGATTACGG AGGAGTCGCT AGAGAATGGT TTTTCCTGAT CTCAAAGGAA
    ATGTTTAACC CTTATTATGG GTTGTTTGAA TATTCTGCTA CGGATAATTA CACCCTACAG
    ATAAATCCAA ACTCTGGATT GTGTAACGAG GATCACCTCT CTTACTTCAA GTTTATTGGC
    CGAGTAGCTG GAATGGCAGT TTATCATGGC AAACTGTTGG ATGGTTTCTT CATCCGCCCA
    TTTTACAAGA TGATGCTACA CAAACCAATA ACACTTCATG ATATGGAATC GGTGGATGGT
    GAATATTACA ATTCACTAAG ATGGATTCTT GAAAATGATC CAACAGAATT GGACCTCAGG
    TTTGTCATAG ATGAAGAACT TTTTGGACAG ACACATCAAC ATGAATTGAA AATTGGTGGA
    TCAGAAATAG TTGTCACCAA TAAGAACAAA AAGGAATATA TTTATCTTGT AATACAGTGG
    CGATTTGTAA ACCGAATCCA GAAGCAAATG GCTGCTTTTA AAGAGGGATT TTTTGAACTA
    ATACCACAGG ATCTCATCAA AATTTTCGAT GAAAATGAAC TAGAGCTTCT TATGTGTGGA
    TTGGGAGATG TCGATGTGAA TGACTGGAGA GAACACACAA AATATAAGAA TGGCTACAGT
    GTGAATCATC AAGTCATACA GTGGTTCTGG AAGGCTGTTC TAATGATGGA TTCAGAAAAA
    AGAATAAGAT TACTTCAGTT TGTCACTGGT ACATCTCGAG TGCCTATGAA TGGATTTGCT
    GAACTCTATG GCTCAAATGG ACCACAGTCA TTTACAGTTG AACAGTGGGG TACCCCGGAA
    AAGCTGCCAA GAGCCCATAC ATGCTTTAAT CGCTTGGACT TGCCACCCTA TGAATCATTT
    GAAGAATTAT GGGATAAACT GCAGATGGCA ATTGAGAACA CTCAAGGCTT TGATGGCGTT
    GATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe28898
    Clone ID Related Accession (Same CDS sequence) XM_005638441.3 , XM_022412613.1 , XM_022412614.1 , XM_005638441.2
    Accession Version XM_005638441.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2346bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase NEDD4 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005638441.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGAGAGAC CATATACGTT TAAGGATTTT GTTCTTCACC CAAGAAGTCA CAAATCAAGA 
    GTTAAAGGTT ATTTGAGATT AAAAATGACT TATTTACCTA AAACCAGTGG CTCAGAAGAA
    GATAACGCAG AACAGGCCGA GGAACTAGAG CCTGGCTGGG TTGTTTTGGA CCAACCGGAT
    GCTGCCTGCC ATTTGCAGCA GCAGCAAGAA CCTTCTCCTC TACCTCCAGG ATGGGAAGAG
    AGGCAGGATA TTCTTGGAAG GACCTACTAT GTAAACCATG AATCTAGAAG AACACAGTGG
    AAAAGACCAA CCGCTCAGGA CAACCTAACT GATGCTGAGA ATGGTAACAT GCAACTGCAA
    GCACAGCGTG CATTTACTAC CAGACGGCAG ATATCTGAGG AAACAGAAAG TGTGGATAAT
    CGAGAGTCTT CCGAGAGCTG GGAAATTATA AGAGAAGATG AAGCCACCAT GTATAGCAAT
    CAGGCCTTCC CATCACCTCC AGCATCAAAT AACTTGGATG TTCAGACTCA TCTTGCAGAA
    GAATTGAATG CCAGACTCAC AATTTGTAGA AACTCAGCCA CCAGCCAGCC AGTATCCAGC
    TCAAATCATT CCAGCAGAAG AGGCAGCTTA CAAGCCTATA CTTTTGAGGA ACAGCCTACA
    CTTCCTGTGC TTTTGCCTAC TTCATCTGGA TTACCACCAG GTTGGGAGGA AAAACAAGAT
    GAAAGAGGAA GATCATATTA TGTAGATCAC AATTCCAGAA CTACTACCTG GACAAAGCCC
    GTTGTACAGG CCACGGTGGA GACCAGTCAG CTGCAGCCAA GCCAGAGTGC GGCATGTCCT
    CAACCACAGG TCCCCTTGAG TGAGTCAGCG CAGCAGGGGA CCCAGGCGGC TGAAATGGAG
    CAAGGATTCC TCCCTAAAGG CTGGGAAGTC CGGCATGCGC CAAATGGGAG GCCTTTCTTT
    ATTGACCACA ACACCAAAAC CACTACCTGG GAAGATCCAA GACTGAAAAT TCCAGCTCAT
    CTGAGAGGAA AGATGTCACT GGATTCTTCC AATGATCTGG GGCCTTTACC TCCAGGATGG
    GAAGAAAGAA CTCATACAGA TGGAAGAATC TTCTACATAA ATCACAATAT AAAAAGAACA
    CAATGGGAAG ATCCTCGGTT GCAAAATGTA GCAATAACTG GACCAGCAGT GCCCTACTCC
    AGGGATTACA AAAGAAAGTA TGAGTTCTTC CGACGAAAGT TGAAGAAGCA GAACGACATT
    CCAAACAAAT TTGAAATGAA ACTTCGCCGT GCAACTGTTC TTGAGGACTC TTACAGGAGG
    ATTATGGGTG TCAAGAGAGC GGATTTCCTC AAGGCTCGAC TGTGGATTGA GTTCGATGGT
    GAAAAGGGAT TAGATTACGG AGGAGTCGCT AGAGAATGGT TTTTCCTGAT CTCAAAGGAA
    ATGTTTAACC CTTATTATGG GTTGTTTGAA TATTCTGCTA CGGATAATTA CACCCTACAG
    ATAAATCCAA ACTCTGGATT GTGTAACGAG GATCACCTCT CTTACTTCAA GTTTATTGGC
    CGAGTAGCTG GAATGGCAGT TTATCATGGC AAACTGTTGG ATGGTTTCTT CATCCGCCCA
    TTTTACAAGA TGATGCTACA CAAACCAATA ACACTTCATG ATATGGAATC GGTGGATGGT
    GAATATTACA ATTCACTAAG ATGGATTCTT GAAAATGATC CAACAGAATT GGACCTCAGG
    TTTGTCATAG ATGAAGAACT TTTTGGACAG ACACATCAAC ATGAATTGAA AATTGGTGGA
    TCAGAAATAG TTGTCACCAA TAAGAACAAA AAGGAATATA TTTATCTTGT AATACAGTGG
    CGATTTGTAA ACCGAATCCA GAAGCAAATG GCTGCTTTTA AAGAGGGATT TTTTGAACTA
    ATACCACAGG ATCTCATCAA AATTTTCGAT GAAAATGAAC TAGAGCTTCT TATGTGTGGA
    TTGGGAGATG TCGATGTGAA TGACTGGAGA GAACACACAA AATATAAGAA TGGCTACAGT
    GTGAATCATC AAGTCATACA GTGGTTCTGG AAGGCTGTTC TAATGATGGA TTCAGAAAAA
    AGAATAAGAT TACTTCAGTT TGTCACTGGT ACATCTCGAG TGCCTATGAA TGGATTTGCT
    GAACTCTATG GCTCAAATGG ACCACAGTCA TTTACAGTTG AACAGTGGGG TACCCCGGAA
    AAGCTGCCAA GAGCCCATAC ATGCTTTAAT CGCTTGGACT TGCCACCCTA TGAATCATTT
    GAAGAATTAT GGGATAAACT GCAGATGGCA ATTGAGAACA CTCAAGGCTT TGATGGCGTT
    GATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005638498.1
    CDS197..2542
    Translation

    Target ORF information:

    RefSeq Version XM_005638441.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005638441.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGAGAGAC CATATACGTT TAAGGATTTT GTTCTTCACC CAAGAAGTCA CAAATCAAGA 
    GTTAAAGGTT ATTTGAGATT AAAAATGACT TATTTACCTA AAACCAGTGG CTCAGAAGAA
    GATAACGCAG AACAGGCCGA GGAACTAGAG CCTGGCTGGG TTGTTTTGGA CCAACCGGAT
    GCTGCCTGCC ATTTGCAGCA GCAGCAAGAA CCTTCTCCTC TACCTCCAGG ATGGGAAGAG
    AGGCAGGATA TTCTTGGAAG GACCTACTAT GTAAACCATG AATCTAGAAG AACACAGTGG
    AAAAGACCAA CCGCTCAGGA CAACCTAACT GATGCTGAGA ATGGTAACAT GCAACTGCAA
    GCACAGCGTG CATTTACTAC CAGACGGCAG ATATCTGAGG AAACAGAAAG TGTGGATAAT
    CGAGAGTCTT CCGAGAGCTG GGAAATTATA AGAGAAGATG AAGCCACCAT GTATAGCAAT
    CAGGCCTTCC CATCACCTCC AGCATCAAAT AACTTGGATG TTCAGACTCA TCTTGCAGAA
    GAATTGAATG CCAGACTCAC AATTTGTAGA AACTCAGCCA CCAGCCAGCC AGTATCCAGC
    TCAAATCATT CCAGCAGAAG AGGCAGCTTA CAAGCCTATA CTTTTGAGGA ACAGCCTACA
    CTTCCTGTGC TTTTGCCTAC TTCATCTGGA TTACCACCAG GTTGGGAGGA AAAACAAGAT
    GAAAGAGGAA GATCATATTA TGTAGATCAC AATTCCAGAA CTACTACCTG GACAAAGCCC
    GTTGTACAGG CCACGGTGGA GACCAGTCAG CTGCAGCCAA GCCAGAGTGC GGCATGTCCT
    CAACCACAGG TCCCCTTGAG TGAGTCAGCG CAGCAGGGGA CCCAGGCGGC TGAAATGGAG
    CAAGGATTCC TCCCTAAAGG CTGGGAAGTC CGGCATGCGC CAAATGGGAG GCCTTTCTTT
    ATTGACCACA ACACCAAAAC CACTACCTGG GAAGATCCAA GACTGAAAAT TCCAGCTCAT
    CTGAGAGGAA AGATGTCACT GGATTCTTCC AATGATCTGG GGCCTTTACC TCCAGGATGG
    GAAGAAAGAA CTCATACAGA TGGAAGAATC TTCTACATAA ATCACAATAT AAAAAGAACA
    CAATGGGAAG ATCCTCGGTT GCAAAATGTA GCAATAACTG GACCAGCAGT GCCCTACTCC
    AGGGATTACA AAAGAAAGTA TGAGTTCTTC CGACGAAAGT TGAAGAAGCA GAACGACATT
    CCAAACAAAT TTGAAATGAA ACTTCGCCGT GCAACTGTTC TTGAGGACTC TTACAGGAGG
    ATTATGGGTG TCAAGAGAGC GGATTTCCTC AAGGCTCGAC TGTGGATTGA GTTCGATGGT
    GAAAAGGGAT TAGATTACGG AGGAGTCGCT AGAGAATGGT TTTTCCTGAT CTCAAAGGAA
    ATGTTTAACC CTTATTATGG GTTGTTTGAA TATTCTGCTA CGGATAATTA CACCCTACAG
    ATAAATCCAA ACTCTGGATT GTGTAACGAG GATCACCTCT CTTACTTCAA GTTTATTGGC
    CGAGTAGCTG GAATGGCAGT TTATCATGGC AAACTGTTGG ATGGTTTCTT CATCCGCCCA
    TTTTACAAGA TGATGCTACA CAAACCAATA ACACTTCATG ATATGGAATC GGTGGATGGT
    GAATATTACA ATTCACTAAG ATGGATTCTT GAAAATGATC CAACAGAATT GGACCTCAGG
    TTTGTCATAG ATGAAGAACT TTTTGGACAG ACACATCAAC ATGAATTGAA AATTGGTGGA
    TCAGAAATAG TTGTCACCAA TAAGAACAAA AAGGAATATA TTTATCTTGT AATACAGTGG
    CGATTTGTAA ACCGAATCCA GAAGCAAATG GCTGCTTTTA AAGAGGGATT TTTTGAACTA
    ATACCACAGG ATCTCATCAA AATTTTCGAT GAAAATGAAC TAGAGCTTCT TATGTGTGGA
    TTGGGAGATG TCGATGTGAA TGACTGGAGA GAACACACAA AATATAAGAA TGGCTACAGT
    GTGAATCATC AAGTCATACA GTGGTTCTGG AAGGCTGTTC TAATGATGGA TTCAGAAAAA
    AGAATAAGAT TACTTCAGTT TGTCACTGGT ACATCTCGAG TGCCTATGAA TGGATTTGCT
    GAACTCTATG GCTCAAATGG ACCACAGTCA TTTACAGTTG AACAGTGGGG TACCCCGGAA
    AAGCTGCCAA GAGCCCATAC ATGCTTTAAT CGCTTGGACT TGCCACCCTA TGAATCATTT
    GAAGAATTAT GGGATAAACT GCAGATGGCA ATTGAGAACA CTCAAGGCTT TGATGGCGTT
    GATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe28893
    Clone ID Related Accession (Same CDS sequence) XM_005638437.1 , XM_005638437.2
    Accession Version XM_005638437.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3972bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase NEDD4 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCACAAA GCTTACGATT GCACTTTGCA GCCAGAAGAA GCAATACTTA TCCTTTGTCA 
    GAAACCTCCG GAGATGATTT GGATAGCAAT GTTCACATGT GCTTCAAAAG ACCAACACGG
    ATTTCAACGT CTAACGTTGT TCAAATGAAG CTGACTCCTA GACAGACTGC ACTAGCTCCG
    TTGATACAGG AAAACGTTAA CTCTCAGGAA AGATCATCTG TTCCTTCACC TGAAAATGTT
    AATAAAAAGA GCAGCTGTCT GCAGATTTCA CTACAACCAA CAAGGTACAG TGGATGTCTT
    CAGTCTGGCA ATGTCTTAGC TGATGGTGAT GATGCTTCAT TTACATCTGT CTTTAAGGAT
    GGCATTTACA ACAATGCTGT GGCTGATAAT GAGCTGAATG CTGTGAATGA TGGTAACCTT
    GTAAGCAGTT CGGCCATTCA CAGTGGGAGC CTCAGTAACT TTTCCACCAG TGAGAATGGG
    TCTTACAGCA GCAATGGTAG TGATTATGGA TCATGCACAA GTATCACAAG TGGTGGTTCC
    TACACAAACA GTATCATCAG TGACAGCAGT GGTTATACTT TTCCACCAAC TGATGATACT
    TTTTTTGGTG GAAATTTATC TTCTGACAGC ACCTCCAATA GAAGTGTGCC AAACAGGAAT
    ACTACTCCAT GTGACATTTT TTCAAGAAGT CCAAGTACCG TTCCTTTTGT CCAGGATGAC
    TTGGAGCATG GACTAGAAAT TATGAAATTG CCAGTGACCA GGAACTCGAA AATTCCACTA
    AAACGTTGTT CATCCTTAGT CATTTTTCCT AGGAGTCCTT TAAATACCCC ACCGACTTCT
    CCAACAAGTA CAGACGTTCT TCCAAGCAAA GGATCCTATC AAACCTCACA CCAATTTATT
    ATTTCTCCTA GTGAAATTGC CCACAGTGAA GATGGTAGTA GCACTAAGGG ATTTCTTTCA
    ACAGCTGTCA ATGGACTTCG GTTATCTAAA ACAATCTGTA CTCCCGGAGA AGTGAGAGAC
    ATACGACCAC TTCACAGGAA GGGTTCATTA CAAAAGAAAA TTGTTATTGC GAATGATAGT
    CTGAATCAGA ATGTCCGTGA AAAGTCGTCT GAAGGGTTTT CTTGTGTTTC AGTGCATTTC
    ACCCAACAAA AAGCAACTAC ATTAGATTGT GAAACAGCAA GTGGTGATTG CAAACCAGAG
    ATGTCAGAAA TCAAGCTTAA TTCTCATTCA GAGTGCATTA AGCTTATGCA GAGGACATCT
    GCATGTTTAC CATCCTCTCA ACACCTAGAT TACCAAATAA ATATCAATGG ACAGTTGGAA
    AGACCAAATT TACAGATAAA CAAAAACCAT GCTATTTTAC AGAGAAGTGT TTCATTGGGA
    GGAACTTACC CAAATGTTTC CTGTTTATCC AGCCTCAAGC ACAATTGTTC TAAGGGGGGA
    CCATCACAAT TACTCATAAA ATTTGCACCT GGAAATGAAG ATAAAGTTGA TAACTTATCA
    AGAGACAGTA ACAGAGATTT CACAAATGAA CTATCTAATT CTTTAAAGCA TTCTTGTAAG
    ACCAGAGATG ATTTCCTAGG TCAAGTGGAT GTTCCTCTTT ATCCATTACC GACAGAAAAT
    CCAAGAATGG AGAGACCATA TACGTTTAAG GATTTTGTTC TTCACCCAAG AAGTCACAAA
    TCAAGAGTTA AAGGTTATTT GAGATTAAAA ATGACTTATT TACCTAAAAC CAGTGGCTCA
    GAAGAAGATA ACGCAGAACA GGCCGAGGAA CTAGAGCCTG GCTGGGTTGT TTTGGACCAA
    CCGGATGCTG CCTGCCATTT GCAGCAGCAG CAAGAACCTT CTCCTCTACC TCCAGGATGG
    GAAGAGAGGC AGGATATTCT TGGAAGGACC TACTATGTAA ACCATGAATC TAGAAGAACA
    CAGTGGAAAA GACCAACCGC TCAGGACAAC CTAACTGATG CTGAGAATGG TAACATGCAA
    CTGCAAGCAC AGCGTGCATT TACTACCAGA CGGCAGATAT CTGAGGAAAC AGAAAGTGTG
    GATAATCGAG AGTCTTCCGA GAGCTGGGAA ATTATAAGAG AAGATGAAGC CACCATGTAT
    AGCAATCAGG CCTTCCCATC ACCTCCAGCA TCAAATAACT TGGATGTTCA GACTCATCTT
    GCAGAAGAAT TGAATGCCAG ACTCACAATT TGTAGAAACT CAGCCACCAG CCAGCCAGTA
    TCCAGCTCAA ATCATTCCAG CAGAAGAGGC AGCTTACAAG CCTATACTTT TGAGGAACAG
    CCTACACTTC CTGTGCTTTT GCCTACTTCA TCTGGATTAC CACCAGGTTG GGAGGAAAAA
    CAAGATGAAA GAGGAAGATC ATATTATGTA GATCACAATT CCAGAACTAC TACCTGGACA
    AAGCCCGTTG TACAGGCCAC GGTGGAGACC AGTCAGCTGC AGCCAAGCCA GAGTGCGGCA
    TGTCCTCAAC CACAGGTCCC CTTGAGTGAG TCAGCGCAGC AGGGGACCCA GGCGGCTGAA
    ATGGAGCAAG GATTCCTCCC TAAAGGCTGG GAAGTCCGGC ATGCGCCAAA TGGGAGGCCT
    TTCTTTATTG ACCACAACAC CAAAACCACT ACCTGGGAAG ATCCAAGACT GAAAATTCCA
    GCTCATCTGA GAGGAAAGAT GTCACTGGAT TCTTCCAATG ATCTGGGGCC TTTACCTCCA
    GGATGGGAAG AAAGAACTCA TACAGATGGA AGAATCTTCT ACATAAATCA CAATATAAAA
    AGAACACAAT GGGAAGATCC TCGGTTGCAA AATGTAGCAA TAACTGGACC AGCAGTGCCC
    TACTCCAGGG ATTACAAAAG AAAGTATGAG TTCTTCCGAC GAAAGTTGAA GAAGCAGAAC
    GACATTCCAA ACAAATTTGA AATGAAACTT CGCCGTGCAA CTGTTCTTGA GGACTCTTAC
    AGGAGGATTA TGGGTGTCAA GAGAGCGGAT TTCCTCAAGG CTCGACTGTG GATTGAGTTC
    GATGGTGAAA AGGGATTAGA TTACGGAGGA GTCGCTAGAG AATGGTTTTT CCTGATCTCA
    AAGGAAATGT TTAACCCTTA TTATGGGTTG TTTGAATATT CTGCTACGGA TAATTACACC
    CTACAGATAA ATCCAAACTC TGGATTGTGT AACGAGGATC ACCTCTCTTA CTTCAAGTTT
    ATTGGCCGAG TAGCTGGAAT GGCAGTTTAT CATGGCAAAC TGTTGGATGG TTTCTTCATC
    CGCCCATTTT ACAAGATGAT GCTACACAAA CCAATAACAC TTCATGATAT GGAATCGGTG
    GATGGTGAAT ATTACAATTC ACTAAGATGG ATTCTTGAAA ATGATCCAAC AGAATTGGAC
    CTCAGGTTTG TCATAGATGA AGAACTTTTT GGACAGACAC ATCAACATGA ATTGAAAATT
    GGTGGATCAG AAATAGTTGT CACCAATAAG AACAAAAAGG AATATATTTA TCTTGTAATA
    CAGTGGCGAT TTGTAAACCG AATCCAGAAG CAAATGGCTG CTTTTAAAGA GGGATTTTTT
    GAACTAATAC CACAGGATCT CATCAAAATT TTCGATGAAA ATGAACTAGA GCTTCTTATG
    TGTGGATTGG GAGATGTCGA TGTGAATGAC TGGAGAGAAC ACACAAAATA TAAGAATGGC
    TACAGTGTGA ATCATCAAGT CATACAGTGG TTCTGGAAGG CTGTTCTAAT GATGGATTCA
    GAAAAAAGAA TAAGATTACT TCAGTTTGTC ACTGGTACAT CTCGAGTGCC TATGAATGGA
    TTTGCTGAAC TCTATGGCTC AAATGGACCA CAGTCATTTA CAGTTGAACA GTGGGGTACC
    CCGGAAAAGC TGCCAAGAGC CCATACATGC TTTAATCGCT TGGACTTGCC ACCCTATGAA
    TCATTTGAAG AATTATGGGA TAAACTGCAG ATGGCAATTG AGAACACTCA AGGCTTTGAT
    GGCGTTGATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005638494.1
    CDS437..4408
    Translation

    Target ORF information:

    RefSeq Version XM_005638437.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005638437.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCACAAA GCTTACGATT GCACTTTGCA GCCAGAAGAA GCAATACTTA TCCTTTGTCA 
    GAAACCTCCG GAGATGATTT GGATAGCAAT GTTCACATGT GCTTCAAAAG ACCAACACGG
    ATTTCAACGT CTAACGTTGT TCAAATGAAG CTGACTCCTA GACAGACTGC ACTAGCTCCG
    TTGATACAGG AAAACGTTAA CTCTCAGGAA AGATCATCTG TTCCTTCACC TGAAAATGTT
    AATAAAAAGA GCAGCTGTCT GCAGATTTCA CTACAACCAA CAAGGTACAG TGGATGTCTT
    CAGTCTGGCA ATGTCTTAGC TGATGGTGAT GATGCTTCAT TTACATCTGT CTTTAAGGAT
    GGCATTTACA ACAATGCTGT GGCTGATAAT GAGCTGAATG CTGTGAATGA TGGTAACCTT
    GTAAGCAGTT CGGCCATTCA CAGTGGGAGC CTCAGTAACT TTTCCACCAG TGAGAATGGG
    TCTTACAGCA GCAATGGTAG TGATTATGGA TCATGCACAA GTATCACAAG TGGTGGTTCC
    TACACAAACA GTATCATCAG TGACAGCAGT GGTTATACTT TTCCACCAAC TGATGATACT
    TTTTTTGGTG GAAATTTATC TTCTGACAGC ACCTCCAATA GAAGTGTGCC AAACAGGAAT
    ACTACTCCAT GTGACATTTT TTCAAGAAGT CCAAGTACCG TTCCTTTTGT CCAGGATGAC
    TTGGAGCATG GACTAGAAAT TATGAAATTG CCAGTGACCA GGAACTCGAA AATTCCACTA
    AAACGTTGTT CATCCTTAGT CATTTTTCCT AGGAGTCCTT TAAATACCCC ACCGACTTCT
    CCAACAAGTA CAGACGTTCT TCCAAGCAAA GGATCCTATC AAACCTCACA CCAATTTATT
    ATTTCTCCTA GTGAAATTGC CCACAGTGAA GATGGTAGTA GCACTAAGGG ATTTCTTTCA
    ACAGCTGTCA ATGGACTTCG GTTATCTAAA ACAATCTGTA CTCCCGGAGA AGTGAGAGAC
    ATACGACCAC TTCACAGGAA GGGTTCATTA CAAAAGAAAA TTGTTATTGC GAATGATAGT
    CTGAATCAGA ATGTCCGTGA AAAGTCGTCT GAAGGGTTTT CTTGTGTTTC AGTGCATTTC
    ACCCAACAAA AAGCAACTAC ATTAGATTGT GAAACAGCAA GTGGTGATTG CAAACCAGAG
    ATGTCAGAAA TCAAGCTTAA TTCTCATTCA GAGTGCATTA AGCTTATGCA GAGGACATCT
    GCATGTTTAC CATCCTCTCA ACACCTAGAT TACCAAATAA ATATCAATGG ACAGTTGGAA
    AGACCAAATT TACAGATAAA CAAAAACCAT GCTATTTTAC AGAGAAGTGT TTCATTGGGA
    GGAACTTACC CAAATGTTTC CTGTTTATCC AGCCTCAAGC ACAATTGTTC TAAGGGGGGA
    CCATCACAAT TACTCATAAA ATTTGCACCT GGAAATGAAG ATAAAGTTGA TAACTTATCA
    AGAGACAGTA ACAGAGATTT CACAAATGAA CTATCTAATT CTTTAAAGCA TTCTTGTAAG
    ACCAGAGATG ATTTCCTAGG TCAAGTGGAT GTTCCTCTTT ATCCATTACC GACAGAAAAT
    CCAAGAATGG AGAGACCATA TACGTTTAAG GATTTTGTTC TTCACCCAAG AAGTCACAAA
    TCAAGAGTTA AAGGTTATTT GAGATTAAAA ATGACTTATT TACCTAAAAC CAGTGGCTCA
    GAAGAAGATA ACGCAGAACA GGCCGAGGAA CTAGAGCCTG GCTGGGTTGT TTTGGACCAA
    CCGGATGCTG CCTGCCATTT GCAGCAGCAG CAAGAACCTT CTCCTCTACC TCCAGGATGG
    GAAGAGAGGC AGGATATTCT TGGAAGGACC TACTATGTAA ACCATGAATC TAGAAGAACA
    CAGTGGAAAA GACCAACCGC TCAGGACAAC CTAACTGATG CTGAGAATGG TAACATGCAA
    CTGCAAGCAC AGCGTGCATT TACTACCAGA CGGCAGATAT CTGAGGAAAC AGAAAGTGTG
    GATAATCGAG AGTCTTCCGA GAGCTGGGAA ATTATAAGAG AAGATGAAGC CACCATGTAT
    AGCAATCAGG CCTTCCCATC ACCTCCAGCA TCAAATAACT TGGATGTTCA GACTCATCTT
    GCAGAAGAAT TGAATGCCAG ACTCACAATT TGTAGAAACT CAGCCACCAG CCAGCCAGTA
    TCCAGCTCAA ATCATTCCAG CAGAAGAGGC AGCTTACAAG CCTATACTTT TGAGGAACAG
    CCTACACTTC CTGTGCTTTT GCCTACTTCA TCTGGATTAC CACCAGGTTG GGAGGAAAAA
    CAAGATGAAA GAGGAAGATC ATATTATGTA GATCACAATT CCAGAACTAC TACCTGGACA
    AAGCCCGTTG TACAGGCCAC GGTGGAGACC AGTCAGCTGC AGCCAAGCCA GAGTGCGGCA
    TGTCCTCAAC CACAGGTCCC CTTGAGTGAG TCAGCGCAGC AGGGGACCCA GGCGGCTGAA
    ATGGAGCAAG GATTCCTCCC TAAAGGCTGG GAAGTCCGGC ATGCGCCAAA TGGGAGGCCT
    TTCTTTATTG ACCACAACAC CAAAACCACT ACCTGGGAAG ATCCAAGACT GAAAATTCCA
    GCTCATCTGA GAGGAAAGAT GTCACTGGAT TCTTCCAATG ATCTGGGGCC TTTACCTCCA
    GGATGGGAAG AAAGAACTCA TACAGATGGA AGAATCTTCT ACATAAATCA CAATATAAAA
    AGAACACAAT GGGAAGATCC TCGGTTGCAA AATGTAGCAA TAACTGGACC AGCAGTGCCC
    TACTCCAGGG ATTACAAAAG AAAGTATGAG TTCTTCCGAC GAAAGTTGAA GAAGCAGAAC
    GACATTCCAA ACAAATTTGA AATGAAACTT CGCCGTGCAA CTGTTCTTGA GGACTCTTAC
    AGGAGGATTA TGGGTGTCAA GAGAGCGGAT TTCCTCAAGG CTCGACTGTG GATTGAGTTC
    GATGGTGAAA AGGGATTAGA TTACGGAGGA GTCGCTAGAG AATGGTTTTT CCTGATCTCA
    AAGGAAATGT TTAACCCTTA TTATGGGTTG TTTGAATATT CTGCTACGGA TAATTACACC
    CTACAGATAA ATCCAAACTC TGGATTGTGT AACGAGGATC ACCTCTCTTA CTTCAAGTTT
    ATTGGCCGAG TAGCTGGAAT GGCAGTTTAT CATGGCAAAC TGTTGGATGG TTTCTTCATC
    CGCCCATTTT ACAAGATGAT GCTACACAAA CCAATAACAC TTCATGATAT GGAATCGGTG
    GATGGTGAAT ATTACAATTC ACTAAGATGG ATTCTTGAAA ATGATCCAAC AGAATTGGAC
    CTCAGGTTTG TCATAGATGA AGAACTTTTT GGACAGACAC ATCAACATGA ATTGAAAATT
    GGTGGATCAG AAATAGTTGT CACCAATAAG AACAAAAAGG AATATATTTA TCTTGTAATA
    CAGTGGCGAT TTGTAAACCG AATCCAGAAG CAAATGGCTG CTTTTAAAGA GGGATTTTTT
    GAACTAATAC CACAGGATCT CATCAAAATT TTCGATGAAA ATGAACTAGA GCTTCTTATG
    TGTGGATTGG GAGATGTCGA TGTGAATGAC TGGAGAGAAC ACACAAAATA TAAGAATGGC
    TACAGTGTGA ATCATCAAGT CATACAGTGG TTCTGGAAGG CTGTTCTAAT GATGGATTCA
    GAAAAAAGAA TAAGATTACT TCAGTTTGTC ACTGGTACAT CTCGAGTGCC TATGAATGGA
    TTTGCTGAAC TCTATGGCTC AAATGGACCA CAGTCATTTA CAGTTGAACA GTGGGGTACC
    CCGGAAAAGC TGCCAAGAGC CCATACATGC TTTAATCGCT TGGACTTGCC ACCCTATGAA
    TCATTTGAAG AATTATGGGA TAAACTGCAG ATGGCAATTG AGAACACTCA AGGCTTTGAT
    GGCGTTGATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe28893
    Clone ID Related Accession (Same CDS sequence) XM_005638437.1 , XM_005638437.2
    Accession Version XM_005638437.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3972bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase NEDD4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005638437.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCACAAA GCTTACGATT GCACTTTGCA GCCAGAAGAA GCAATACTTA TCCTTTGTCA 
    GAAACCTCCG GAGATGATTT GGATAGCAAT GTTCACATGT GCTTCAAAAG ACCAACACGG
    ATTTCAACGT CTAACGTTGT TCAAATGAAG CTGACTCCTA GACAGACTGC ACTAGCTCCG
    TTGATACAGG AAAACGTTAA CTCTCAGGAA AGATCATCTG TTCCTTCACC TGAAAATGTT
    AATAAAAAGA GCAGCTGTCT GCAGATTTCA CTACAACCAA CAAGGTACAG TGGATGTCTT
    CAGTCTGGCA ATGTCTTAGC TGATGGTGAT GATGCTTCAT TTACATCTGT CTTTAAGGAT
    GGCATTTACA ACAATGCTGT GGCTGATAAT GAGCTGAATG CTGTGAATGA TGGTAACCTT
    GTAAGCAGTT CGGCCATTCA CAGTGGGAGC CTCAGTAACT TTTCCACCAG TGAGAATGGG
    TCTTACAGCA GCAATGGTAG TGATTATGGA TCATGCACAA GTATCACAAG TGGTGGTTCC
    TACACAAACA GTATCATCAG TGACAGCAGT GGTTATACTT TTCCACCAAC TGATGATACT
    TTTTTTGGTG GAAATTTATC TTCTGACAGC ACCTCCAATA GAAGTGTGCC AAACAGGAAT
    ACTACTCCAT GTGACATTTT TTCAAGAAGT CCAAGTACCG TTCCTTTTGT CCAGGATGAC
    TTGGAGCATG GACTAGAAAT TATGAAATTG CCAGTGACCA GGAACTCGAA AATTCCACTA
    AAACGTTGTT CATCCTTAGT CATTTTTCCT AGGAGTCCTT TAAATACCCC ACCGACTTCT
    CCAACAAGTA CAGACGTTCT TCCAAGCAAA GGATCCTATC AAACCTCACA CCAATTTATT
    ATTTCTCCTA GTGAAATTGC CCACAGTGAA GATGGTAGTA GCACTAAGGG ATTTCTTTCA
    ACAGCTGTCA ATGGACTTCG GTTATCTAAA ACAATCTGTA CTCCCGGAGA AGTGAGAGAC
    ATACGACCAC TTCACAGGAA GGGTTCATTA CAAAAGAAAA TTGTTATTGC GAATGATAGT
    CTGAATCAGA ATGTCCGTGA AAAGTCGTCT GAAGGGTTTT CTTGTGTTTC AGTGCATTTC
    ACCCAACAAA AAGCAACTAC ATTAGATTGT GAAACAGCAA GTGGTGATTG CAAACCAGAG
    ATGTCAGAAA TCAAGCTTAA TTCTCATTCA GAGTGCATTA AGCTTATGCA GAGGACATCT
    GCATGTTTAC CATCCTCTCA ACACCTAGAT TACCAAATAA ATATCAATGG ACAGTTGGAA
    AGACCAAATT TACAGATAAA CAAAAACCAT GCTATTTTAC AGAGAAGTGT TTCATTGGGA
    GGAACTTACC CAAATGTTTC CTGTTTATCC AGCCTCAAGC ACAATTGTTC TAAGGGGGGA
    CCATCACAAT TACTCATAAA ATTTGCACCT GGAAATGAAG ATAAAGTTGA TAACTTATCA
    AGAGACAGTA ACAGAGATTT CACAAATGAA CTATCTAATT CTTTAAAGCA TTCTTGTAAG
    ACCAGAGATG ATTTCCTAGG TCAAGTGGAT GTTCCTCTTT ATCCATTACC GACAGAAAAT
    CCAAGAATGG AGAGACCATA TACGTTTAAG GATTTTGTTC TTCACCCAAG AAGTCACAAA
    TCAAGAGTTA AAGGTTATTT GAGATTAAAA ATGACTTATT TACCTAAAAC CAGTGGCTCA
    GAAGAAGATA ACGCAGAACA GGCCGAGGAA CTAGAGCCTG GCTGGGTTGT TTTGGACCAA
    CCGGATGCTG CCTGCCATTT GCAGCAGCAG CAAGAACCTT CTCCTCTACC TCCAGGATGG
    GAAGAGAGGC AGGATATTCT TGGAAGGACC TACTATGTAA ACCATGAATC TAGAAGAACA
    CAGTGGAAAA GACCAACCGC TCAGGACAAC CTAACTGATG CTGAGAATGG TAACATGCAA
    CTGCAAGCAC AGCGTGCATT TACTACCAGA CGGCAGATAT CTGAGGAAAC AGAAAGTGTG
    GATAATCGAG AGTCTTCCGA GAGCTGGGAA ATTATAAGAG AAGATGAAGC CACCATGTAT
    AGCAATCAGG CCTTCCCATC ACCTCCAGCA TCAAATAACT TGGATGTTCA GACTCATCTT
    GCAGAAGAAT TGAATGCCAG ACTCACAATT TGTAGAAACT CAGCCACCAG CCAGCCAGTA
    TCCAGCTCAA ATCATTCCAG CAGAAGAGGC AGCTTACAAG CCTATACTTT TGAGGAACAG
    CCTACACTTC CTGTGCTTTT GCCTACTTCA TCTGGATTAC CACCAGGTTG GGAGGAAAAA
    CAAGATGAAA GAGGAAGATC ATATTATGTA GATCACAATT CCAGAACTAC TACCTGGACA
    AAGCCCGTTG TACAGGCCAC GGTGGAGACC AGTCAGCTGC AGCCAAGCCA GAGTGCGGCA
    TGTCCTCAAC CACAGGTCCC CTTGAGTGAG TCAGCGCAGC AGGGGACCCA GGCGGCTGAA
    ATGGAGCAAG GATTCCTCCC TAAAGGCTGG GAAGTCCGGC ATGCGCCAAA TGGGAGGCCT
    TTCTTTATTG ACCACAACAC CAAAACCACT ACCTGGGAAG ATCCAAGACT GAAAATTCCA
    GCTCATCTGA GAGGAAAGAT GTCACTGGAT TCTTCCAATG ATCTGGGGCC TTTACCTCCA
    GGATGGGAAG AAAGAACTCA TACAGATGGA AGAATCTTCT ACATAAATCA CAATATAAAA
    AGAACACAAT GGGAAGATCC TCGGTTGCAA AATGTAGCAA TAACTGGACC AGCAGTGCCC
    TACTCCAGGG ATTACAAAAG AAAGTATGAG TTCTTCCGAC GAAAGTTGAA GAAGCAGAAC
    GACATTCCAA ACAAATTTGA AATGAAACTT CGCCGTGCAA CTGTTCTTGA GGACTCTTAC
    AGGAGGATTA TGGGTGTCAA GAGAGCGGAT TTCCTCAAGG CTCGACTGTG GATTGAGTTC
    GATGGTGAAA AGGGATTAGA TTACGGAGGA GTCGCTAGAG AATGGTTTTT CCTGATCTCA
    AAGGAAATGT TTAACCCTTA TTATGGGTTG TTTGAATATT CTGCTACGGA TAATTACACC
    CTACAGATAA ATCCAAACTC TGGATTGTGT AACGAGGATC ACCTCTCTTA CTTCAAGTTT
    ATTGGCCGAG TAGCTGGAAT GGCAGTTTAT CATGGCAAAC TGTTGGATGG TTTCTTCATC
    CGCCCATTTT ACAAGATGAT GCTACACAAA CCAATAACAC TTCATGATAT GGAATCGGTG
    GATGGTGAAT ATTACAATTC ACTAAGATGG ATTCTTGAAA ATGATCCAAC AGAATTGGAC
    CTCAGGTTTG TCATAGATGA AGAACTTTTT GGACAGACAC ATCAACATGA ATTGAAAATT
    GGTGGATCAG AAATAGTTGT CACCAATAAG AACAAAAAGG AATATATTTA TCTTGTAATA
    CAGTGGCGAT TTGTAAACCG AATCCAGAAG CAAATGGCTG CTTTTAAAGA GGGATTTTTT
    GAACTAATAC CACAGGATCT CATCAAAATT TTCGATGAAA ATGAACTAGA GCTTCTTATG
    TGTGGATTGG GAGATGTCGA TGTGAATGAC TGGAGAGAAC ACACAAAATA TAAGAATGGC
    TACAGTGTGA ATCATCAAGT CATACAGTGG TTCTGGAAGG CTGTTCTAAT GATGGATTCA
    GAAAAAAGAA TAAGATTACT TCAGTTTGTC ACTGGTACAT CTCGAGTGCC TATGAATGGA
    TTTGCTGAAC TCTATGGCTC AAATGGACCA CAGTCATTTA CAGTTGAACA GTGGGGTACC
    CCGGAAAAGC TGCCAAGAGC CCATACATGC TTTAATCGCT TGGACTTGCC ACCCTATGAA
    TCATTTGAAG AATTATGGGA TAAACTGCAG ATGGCAATTG AGAACACTCA AGGCTTTGAT
    GGCGTTGATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005638494.1
    CDS438..4409
    Translation

    Target ORF information:

    RefSeq Version XM_005638437.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005638437.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCACAAA GCTTACGATT GCACTTTGCA GCCAGAAGAA GCAATACTTA TCCTTTGTCA 
    GAAACCTCCG GAGATGATTT GGATAGCAAT GTTCACATGT GCTTCAAAAG ACCAACACGG
    ATTTCAACGT CTAACGTTGT TCAAATGAAG CTGACTCCTA GACAGACTGC ACTAGCTCCG
    TTGATACAGG AAAACGTTAA CTCTCAGGAA AGATCATCTG TTCCTTCACC TGAAAATGTT
    AATAAAAAGA GCAGCTGTCT GCAGATTTCA CTACAACCAA CAAGGTACAG TGGATGTCTT
    CAGTCTGGCA ATGTCTTAGC TGATGGTGAT GATGCTTCAT TTACATCTGT CTTTAAGGAT
    GGCATTTACA ACAATGCTGT GGCTGATAAT GAGCTGAATG CTGTGAATGA TGGTAACCTT
    GTAAGCAGTT CGGCCATTCA CAGTGGGAGC CTCAGTAACT TTTCCACCAG TGAGAATGGG
    TCTTACAGCA GCAATGGTAG TGATTATGGA TCATGCACAA GTATCACAAG TGGTGGTTCC
    TACACAAACA GTATCATCAG TGACAGCAGT GGTTATACTT TTCCACCAAC TGATGATACT
    TTTTTTGGTG GAAATTTATC TTCTGACAGC ACCTCCAATA GAAGTGTGCC AAACAGGAAT
    ACTACTCCAT GTGACATTTT TTCAAGAAGT CCAAGTACCG TTCCTTTTGT CCAGGATGAC
    TTGGAGCATG GACTAGAAAT TATGAAATTG CCAGTGACCA GGAACTCGAA AATTCCACTA
    AAACGTTGTT CATCCTTAGT CATTTTTCCT AGGAGTCCTT TAAATACCCC ACCGACTTCT
    CCAACAAGTA CAGACGTTCT TCCAAGCAAA GGATCCTATC AAACCTCACA CCAATTTATT
    ATTTCTCCTA GTGAAATTGC CCACAGTGAA GATGGTAGTA GCACTAAGGG ATTTCTTTCA
    ACAGCTGTCA ATGGACTTCG GTTATCTAAA ACAATCTGTA CTCCCGGAGA AGTGAGAGAC
    ATACGACCAC TTCACAGGAA GGGTTCATTA CAAAAGAAAA TTGTTATTGC GAATGATAGT
    CTGAATCAGA ATGTCCGTGA AAAGTCGTCT GAAGGGTTTT CTTGTGTTTC AGTGCATTTC
    ACCCAACAAA AAGCAACTAC ATTAGATTGT GAAACAGCAA GTGGTGATTG CAAACCAGAG
    ATGTCAGAAA TCAAGCTTAA TTCTCATTCA GAGTGCATTA AGCTTATGCA GAGGACATCT
    GCATGTTTAC CATCCTCTCA ACACCTAGAT TACCAAATAA ATATCAATGG ACAGTTGGAA
    AGACCAAATT TACAGATAAA CAAAAACCAT GCTATTTTAC AGAGAAGTGT TTCATTGGGA
    GGAACTTACC CAAATGTTTC CTGTTTATCC AGCCTCAAGC ACAATTGTTC TAAGGGGGGA
    CCATCACAAT TACTCATAAA ATTTGCACCT GGAAATGAAG ATAAAGTTGA TAACTTATCA
    AGAGACAGTA ACAGAGATTT CACAAATGAA CTATCTAATT CTTTAAAGCA TTCTTGTAAG
    ACCAGAGATG ATTTCCTAGG TCAAGTGGAT GTTCCTCTTT ATCCATTACC GACAGAAAAT
    CCAAGAATGG AGAGACCATA TACGTTTAAG GATTTTGTTC TTCACCCAAG AAGTCACAAA
    TCAAGAGTTA AAGGTTATTT GAGATTAAAA ATGACTTATT TACCTAAAAC CAGTGGCTCA
    GAAGAAGATA ACGCAGAACA GGCCGAGGAA CTAGAGCCTG GCTGGGTTGT TTTGGACCAA
    CCGGATGCTG CCTGCCATTT GCAGCAGCAG CAAGAACCTT CTCCTCTACC TCCAGGATGG
    GAAGAGAGGC AGGATATTCT TGGAAGGACC TACTATGTAA ACCATGAATC TAGAAGAACA
    CAGTGGAAAA GACCAACCGC TCAGGACAAC CTAACTGATG CTGAGAATGG TAACATGCAA
    CTGCAAGCAC AGCGTGCATT TACTACCAGA CGGCAGATAT CTGAGGAAAC AGAAAGTGTG
    GATAATCGAG AGTCTTCCGA GAGCTGGGAA ATTATAAGAG AAGATGAAGC CACCATGTAT
    AGCAATCAGG CCTTCCCATC ACCTCCAGCA TCAAATAACT TGGATGTTCA GACTCATCTT
    GCAGAAGAAT TGAATGCCAG ACTCACAATT TGTAGAAACT CAGCCACCAG CCAGCCAGTA
    TCCAGCTCAA ATCATTCCAG CAGAAGAGGC AGCTTACAAG CCTATACTTT TGAGGAACAG
    CCTACACTTC CTGTGCTTTT GCCTACTTCA TCTGGATTAC CACCAGGTTG GGAGGAAAAA
    CAAGATGAAA GAGGAAGATC ATATTATGTA GATCACAATT CCAGAACTAC TACCTGGACA
    AAGCCCGTTG TACAGGCCAC GGTGGAGACC AGTCAGCTGC AGCCAAGCCA GAGTGCGGCA
    TGTCCTCAAC CACAGGTCCC CTTGAGTGAG TCAGCGCAGC AGGGGACCCA GGCGGCTGAA
    ATGGAGCAAG GATTCCTCCC TAAAGGCTGG GAAGTCCGGC ATGCGCCAAA TGGGAGGCCT
    TTCTTTATTG ACCACAACAC CAAAACCACT ACCTGGGAAG ATCCAAGACT GAAAATTCCA
    GCTCATCTGA GAGGAAAGAT GTCACTGGAT TCTTCCAATG ATCTGGGGCC TTTACCTCCA
    GGATGGGAAG AAAGAACTCA TACAGATGGA AGAATCTTCT ACATAAATCA CAATATAAAA
    AGAACACAAT GGGAAGATCC TCGGTTGCAA AATGTAGCAA TAACTGGACC AGCAGTGCCC
    TACTCCAGGG ATTACAAAAG AAAGTATGAG TTCTTCCGAC GAAAGTTGAA GAAGCAGAAC
    GACATTCCAA ACAAATTTGA AATGAAACTT CGCCGTGCAA CTGTTCTTGA GGACTCTTAC
    AGGAGGATTA TGGGTGTCAA GAGAGCGGAT TTCCTCAAGG CTCGACTGTG GATTGAGTTC
    GATGGTGAAA AGGGATTAGA TTACGGAGGA GTCGCTAGAG AATGGTTTTT CCTGATCTCA
    AAGGAAATGT TTAACCCTTA TTATGGGTTG TTTGAATATT CTGCTACGGA TAATTACACC
    CTACAGATAA ATCCAAACTC TGGATTGTGT AACGAGGATC ACCTCTCTTA CTTCAAGTTT
    ATTGGCCGAG TAGCTGGAAT GGCAGTTTAT CATGGCAAAC TGTTGGATGG TTTCTTCATC
    CGCCCATTTT ACAAGATGAT GCTACACAAA CCAATAACAC TTCATGATAT GGAATCGGTG
    GATGGTGAAT ATTACAATTC ACTAAGATGG ATTCTTGAAA ATGATCCAAC AGAATTGGAC
    CTCAGGTTTG TCATAGATGA AGAACTTTTT GGACAGACAC ATCAACATGA ATTGAAAATT
    GGTGGATCAG AAATAGTTGT CACCAATAAG AACAAAAAGG AATATATTTA TCTTGTAATA
    CAGTGGCGAT TTGTAAACCG AATCCAGAAG CAAATGGCTG CTTTTAAAGA GGGATTTTTT
    GAACTAATAC CACAGGATCT CATCAAAATT TTCGATGAAA ATGAACTAGA GCTTCTTATG
    TGTGGATTGG GAGATGTCGA TGTGAATGAC TGGAGAGAAC ACACAAAATA TAAGAATGGC
    TACAGTGTGA ATCATCAAGT CATACAGTGG TTCTGGAAGG CTGTTCTAAT GATGGATTCA
    GAAAAAAGAA TAAGATTACT TCAGTTTGTC ACTGGTACAT CTCGAGTGCC TATGAATGGA
    TTTGCTGAAC TCTATGGCTC AAATGGACCA CAGTCATTTA CAGTTGAACA GTGGGGTACC
    CCGGAAAAGC TGCCAAGAGC CCATACATGC TTTAATCGCT TGGACTTGCC ACCCTATGAA
    TCATTTGAAG AATTATGGGA TAAACTGCAG ATGGCAATTG AGAACACTCA AGGCTTTGAT
    GGCGTTGATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.