SPAST cDNA ORF clone, Canis lupus familiaris(dog)

The following SPAST gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPAST cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe18860 XM_845880.5
Latest version!
Canis lupus familiaris spastin (SPAST), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
OCe18861 XM_005630315.3
Latest version!
Canis lupus familiaris spastin (SPAST), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
OCe18862 XM_857738.5
Latest version!
Canis lupus familiaris spastin (SPAST), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
View Old Accession Versions >>
OCe18861 XM_005630315.2 Canis lupus familiaris spastin (SPAST), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
OCe18860 XM_845880.4 Canis lupus familiaris spastin (SPAST), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
OCe18862 XM_857738.4 Canis lupus familiaris spastin (SPAST), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe18860
    Clone ID Related Accession (Same CDS sequence) XM_845880.4 , XM_845880.5
    Accession Version XM_845880.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1875bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product spastin isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_845880.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAGTTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGGCGG CGGCAGCAGC 
    GGCAGCAGCG GCAGCAGCCC GGTGCCTCCC AGGCCTCCGC CCCCCTGCCT GGCGCCCGCG
    CGTCCCGCCC CCAGGCCGGC CCCCCCGCCC GAGTCTCCGC ATAAGCGGAA CCTGTACTAC
    TTCTCCTACC CGCTGTTCCT AGGCTTCGCG CTGCTGCGTC TGGTCGCCTT CCACCTGGGG
    CTCCTCTTCG TGTGGCTCTG CCAGCGCTTC TCCCGCGCCC TCATGGCTGC CAAGAGGAGC
    TCCCGGGCCG CGCCGGCGCC GGCCTCGGCC TCGCCCCCCG CGCCGGTGCC GGGCGGGGAG
    GCCGAGCGCG TCCGAGCCTT CCACAAGCAG GCCTTCGAGT ACATCTCCGT TGCCCTGCGC
    ATCGATGAGG ACGAGAAAGC AGGACAAAAA GAGCAAGCTG TGGAATGGTA TAAGAAAGGT
    ATTGAAGAAC TAGAAAAAGG AATAGCTGTC ATAGTTACAG GACAAGGTGA ACAGTATGAA
    AGAGCTAGAC GCCTTCAAGC TAAAATGATG ACTAATTTGG TTATGGCCAA GGACCGTTTA
    CAACTTCTAG AGAAGCTGCA ACCAGTTTTG CAATTTTCCA AGTCACAAAC GGATGTCTAT
    AATGACAGTA CTAACTTGAC ATGCCGAAAC GGACATCTCC AGTCAGAAAG TGGAGCTGTT
    CCAAAGAGAA AAGATCCCTT AACACACACT AGTAATTCAC TGCCTCGTTC AAAAACAGTT
    ATGAAGACTG GATCCACAGG TTTGTCAGGC CACCACAGAG CACCTAGTTG TAGTGGCTTA
    TCCATGGTTT CTGGAGTGAG ACAGGGACCT GGTCCTGTAA CTGGAACTCA TAAGAGTACG
    CCAAAAACAA ATAGAACAAA TAAGCCTTCT ACCCCTACAA CTGCTGCTCG TAAAAAGAAA
    GACTTGAAGA ATTTTAGGAA TGTGGACAGC AATCTTGCTA ACCTTATAAT GAACGAAATT
    GTGGACAATG GAACAGCTGT TAAATTTGAT GATATAGCTG GGCAAGAATT GGCAAAACAA
    GCCTTGCAAG AAATTGTTAT TCTTCCTTCT CTGAGGCCTG AGTTGTTCAC AGGGCTTAGA
    GCTCCTGCCA GAGGATTGTT ACTCTTTGGT CCACCTGGAA ATGGGAAAAC AATGCTGGCT
    AAAGCAGTAG CTGCAGAATC CAATGCAACC TTCTTTAATA TAAGTGCTGC AAGTTTAACT
    TCAAAATATG TAGGAGAAGG AGAGAAATTG GTGAGAGCTC TTTTTGCTGT GGCTCGAGAA
    CTTCAGCCTT CAATAATTTT TATAGATGAA GTTGATAGCC TTTTGTGTGA AAGAAGAGAA
    GGAGAGCATG ATGCTAGTAG ACGTCTAAAA ACTGAATTTC TAATAGAATT TGATGGTGTA
    CAATCTGCTG GAGATGACAG AGTGCTTGTA ATGGGTGCAA CTAATAGGCC ACAAGAGCTT
    GATGAGGCTG TTCTCAGGCG TTTCATCAAA CGGGTATATG TGTCTTTGCC AAATGAGGAG
    ACACGACTAC TTTTACTTAA AAATCTATTA TGTAAACAAG GAAGCCCATT GACCCAAAAA
    GAACTAGCAC AACTTGCTAG AATGACCGAT GGGTATTCAG GAAGTGATCT AACAGCTTTG
    GCAAAAGATG CAGCATTGGG TCCTATCCGA GAACTGAAAC CAGAACAAGT AAAGAATATG
    TCTGCCAGTG AGATGAGAAA TATTCGATTA TCTGACTTCA CTGAATCCTT GAAAAAGATA
    AAGCGCAGCG TGAGCCCTCA AACCTTAGAA GCATACATAC GTTGGAACAA GGACTTTGGC
    GATACCACTG TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_850973.1
    CDS263..2137
    Translation

    Target ORF information:

    RefSeq Version XM_845880.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris spastin (SPAST), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_845880.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAGTTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGGCGG CGGCAGCAGC 
    GGCAGCAGCG GCAGCAGCCC GGTGCCTCCC AGGCCTCCGC CCCCCTGCCT GGCGCCCGCG
    CGTCCCGCCC CCAGGCCGGC CCCCCCGCCC GAGTCTCCGC ATAAGCGGAA CCTGTACTAC
    TTCTCCTACC CGCTGTTCCT AGGCTTCGCG CTGCTGCGTC TGGTCGCCTT CCACCTGGGG
    CTCCTCTTCG TGTGGCTCTG CCAGCGCTTC TCCCGCGCCC TCATGGCTGC CAAGAGGAGC
    TCCCGGGCCG CGCCGGCGCC GGCCTCGGCC TCGCCCCCCG CGCCGGTGCC GGGCGGGGAG
    GCCGAGCGCG TCCGAGCCTT CCACAAGCAG GCCTTCGAGT ACATCTCCGT TGCCCTGCGC
    ATCGATGAGG ACGAGAAAGC AGGACAAAAA GAGCAAGCTG TGGAATGGTA TAAGAAAGGT
    ATTGAAGAAC TAGAAAAAGG AATAGCTGTC ATAGTTACAG GACAAGGTGA ACAGTATGAA
    AGAGCTAGAC GCCTTCAAGC TAAAATGATG ACTAATTTGG TTATGGCCAA GGACCGTTTA
    CAACTTCTAG AGAAGCTGCA ACCAGTTTTG CAATTTTCCA AGTCACAAAC GGATGTCTAT
    AATGACAGTA CTAACTTGAC ATGCCGAAAC GGACATCTCC AGTCAGAAAG TGGAGCTGTT
    CCAAAGAGAA AAGATCCCTT AACACACACT AGTAATTCAC TGCCTCGTTC AAAAACAGTT
    ATGAAGACTG GATCCACAGG TTTGTCAGGC CACCACAGAG CACCTAGTTG TAGTGGCTTA
    TCCATGGTTT CTGGAGTGAG ACAGGGACCT GGTCCTGTAA CTGGAACTCA TAAGAGTACG
    CCAAAAACAA ATAGAACAAA TAAGCCTTCT ACCCCTACAA CTGCTGCTCG TAAAAAGAAA
    GACTTGAAGA ATTTTAGGAA TGTGGACAGC AATCTTGCTA ACCTTATAAT GAACGAAATT
    GTGGACAATG GAACAGCTGT TAAATTTGAT GATATAGCTG GGCAAGAATT GGCAAAACAA
    GCCTTGCAAG AAATTGTTAT TCTTCCTTCT CTGAGGCCTG AGTTGTTCAC AGGGCTTAGA
    GCTCCTGCCA GAGGATTGTT ACTCTTTGGT CCACCTGGAA ATGGGAAAAC AATGCTGGCT
    AAAGCAGTAG CTGCAGAATC CAATGCAACC TTCTTTAATA TAAGTGCTGC AAGTTTAACT
    TCAAAATATG TAGGAGAAGG AGAGAAATTG GTGAGAGCTC TTTTTGCTGT GGCTCGAGAA
    CTTCAGCCTT CAATAATTTT TATAGATGAA GTTGATAGCC TTTTGTGTGA AAGAAGAGAA
    GGAGAGCATG ATGCTAGTAG ACGTCTAAAA ACTGAATTTC TAATAGAATT TGATGGTGTA
    CAATCTGCTG GAGATGACAG AGTGCTTGTA ATGGGTGCAA CTAATAGGCC ACAAGAGCTT
    GATGAGGCTG TTCTCAGGCG TTTCATCAAA CGGGTATATG TGTCTTTGCC AAATGAGGAG
    ACACGACTAC TTTTACTTAA AAATCTATTA TGTAAACAAG GAAGCCCATT GACCCAAAAA
    GAACTAGCAC AACTTGCTAG AATGACCGAT GGGTATTCAG GAAGTGATCT AACAGCTTTG
    GCAAAAGATG CAGCATTGGG TCCTATCCGA GAACTGAAAC CAGAACAAGT AAAGAATATG
    TCTGCCAGTG AGATGAGAAA TATTCGATTA TCTGACTTCA CTGAATCCTT GAAAAAGATA
    AAGCGCAGCG TGAGCCCTCA AACCTTAGAA GCATACATAC GTTGGAACAA GGACTTTGGC
    GATACCACTG TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe18860
    Clone ID Related Accession (Same CDS sequence) XM_845880.4 , XM_845880.5
    Accession Version XM_845880.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1875bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product spastin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_845880.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAGTTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGGCGG CGGCAGCAGC 
    GGCAGCAGCG GCAGCAGCCC GGTGCCTCCC AGGCCTCCGC CCCCCTGCCT GGCGCCCGCG
    CGTCCCGCCC CCAGGCCGGC CCCCCCGCCC GAGTCTCCGC ATAAGCGGAA CCTGTACTAC
    TTCTCCTACC CGCTGTTCCT AGGCTTCGCG CTGCTGCGTC TGGTCGCCTT CCACCTGGGG
    CTCCTCTTCG TGTGGCTCTG CCAGCGCTTC TCCCGCGCCC TCATGGCTGC CAAGAGGAGC
    TCCCGGGCCG CGCCGGCGCC GGCCTCGGCC TCGCCCCCCG CGCCGGTGCC GGGCGGGGAG
    GCCGAGCGCG TCCGAGCCTT CCACAAGCAG GCCTTCGAGT ACATCTCCGT TGCCCTGCGC
    ATCGATGAGG ACGAGAAAGC AGGACAAAAA GAGCAAGCTG TGGAATGGTA TAAGAAAGGT
    ATTGAAGAAC TAGAAAAAGG AATAGCTGTC ATAGTTACAG GACAAGGTGA ACAGTATGAA
    AGAGCTAGAC GCCTTCAAGC TAAAATGATG ACTAATTTGG TTATGGCCAA GGACCGTTTA
    CAACTTCTAG AGAAGCTGCA ACCAGTTTTG CAATTTTCCA AGTCACAAAC GGATGTCTAT
    AATGACAGTA CTAACTTGAC ATGCCGAAAC GGACATCTCC AGTCAGAAAG TGGAGCTGTT
    CCAAAGAGAA AAGATCCCTT AACACACACT AGTAATTCAC TGCCTCGTTC AAAAACAGTT
    ATGAAGACTG GATCCACAGG TTTGTCAGGC CACCACAGAG CACCTAGTTG TAGTGGCTTA
    TCCATGGTTT CTGGAGTGAG ACAGGGACCT GGTCCTGTAA CTGGAACTCA TAAGAGTACG
    CCAAAAACAA ATAGAACAAA TAAGCCTTCT ACCCCTACAA CTGCTGCTCG TAAAAAGAAA
    GACTTGAAGA ATTTTAGGAA TGTGGACAGC AATCTTGCTA ACCTTATAAT GAACGAAATT
    GTGGACAATG GAACAGCTGT TAAATTTGAT GATATAGCTG GGCAAGAATT GGCAAAACAA
    GCCTTGCAAG AAATTGTTAT TCTTCCTTCT CTGAGGCCTG AGTTGTTCAC AGGGCTTAGA
    GCTCCTGCCA GAGGATTGTT ACTCTTTGGT CCACCTGGAA ATGGGAAAAC AATGCTGGCT
    AAAGCAGTAG CTGCAGAATC CAATGCAACC TTCTTTAATA TAAGTGCTGC AAGTTTAACT
    TCAAAATATG TAGGAGAAGG AGAGAAATTG GTGAGAGCTC TTTTTGCTGT GGCTCGAGAA
    CTTCAGCCTT CAATAATTTT TATAGATGAA GTTGATAGCC TTTTGTGTGA AAGAAGAGAA
    GGAGAGCATG ATGCTAGTAG ACGTCTAAAA ACTGAATTTC TAATAGAATT TGATGGTGTA
    CAATCTGCTG GAGATGACAG AGTGCTTGTA ATGGGTGCAA CTAATAGGCC ACAAGAGCTT
    GATGAGGCTG TTCTCAGGCG TTTCATCAAA CGGGTATATG TGTCTTTGCC AAATGAGGAG
    ACACGACTAC TTTTACTTAA AAATCTATTA TGTAAACAAG GAAGCCCATT GACCCAAAAA
    GAACTAGCAC AACTTGCTAG AATGACCGAT GGGTATTCAG GAAGTGATCT AACAGCTTTG
    GCAAAAGATG CAGCATTGGG TCCTATCCGA GAACTGAAAC CAGAACAAGT AAAGAATATG
    TCTGCCAGTG AGATGAGAAA TATTCGATTA TCTGACTTCA CTGAATCCTT GAAAAAGATA
    AAGCGCAGCG TGAGCCCTCA AACCTTAGAA GCATACATAC GTTGGAACAA GGACTTTGGC
    GATACCACTG TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_850973.1
    CDS253..2127
    Translation

    Target ORF information:

    RefSeq Version XM_845880.5
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris spastin (SPAST), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_845880.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAGTTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGGCGG CGGCAGCAGC 
    GGCAGCAGCG GCAGCAGCCC GGTGCCTCCC AGGCCTCCGC CCCCCTGCCT GGCGCCCGCG
    CGTCCCGCCC CCAGGCCGGC CCCCCCGCCC GAGTCTCCGC ATAAGCGGAA CCTGTACTAC
    TTCTCCTACC CGCTGTTCCT AGGCTTCGCG CTGCTGCGTC TGGTCGCCTT CCACCTGGGG
    CTCCTCTTCG TGTGGCTCTG CCAGCGCTTC TCCCGCGCCC TCATGGCTGC CAAGAGGAGC
    TCCCGGGCCG CGCCGGCGCC GGCCTCGGCC TCGCCCCCCG CGCCGGTGCC GGGCGGGGAG
    GCCGAGCGCG TCCGAGCCTT CCACAAGCAG GCCTTCGAGT ACATCTCCGT TGCCCTGCGC
    ATCGATGAGG ACGAGAAAGC AGGACAAAAA GAGCAAGCTG TGGAATGGTA TAAGAAAGGT
    ATTGAAGAAC TAGAAAAAGG AATAGCTGTC ATAGTTACAG GACAAGGTGA ACAGTATGAA
    AGAGCTAGAC GCCTTCAAGC TAAAATGATG ACTAATTTGG TTATGGCCAA GGACCGTTTA
    CAACTTCTAG AGAAGCTGCA ACCAGTTTTG CAATTTTCCA AGTCACAAAC GGATGTCTAT
    AATGACAGTA CTAACTTGAC ATGCCGAAAC GGACATCTCC AGTCAGAAAG TGGAGCTGTT
    CCAAAGAGAA AAGATCCCTT AACACACACT AGTAATTCAC TGCCTCGTTC AAAAACAGTT
    ATGAAGACTG GATCCACAGG TTTGTCAGGC CACCACAGAG CACCTAGTTG TAGTGGCTTA
    TCCATGGTTT CTGGAGTGAG ACAGGGACCT GGTCCTGTAA CTGGAACTCA TAAGAGTACG
    CCAAAAACAA ATAGAACAAA TAAGCCTTCT ACCCCTACAA CTGCTGCTCG TAAAAAGAAA
    GACTTGAAGA ATTTTAGGAA TGTGGACAGC AATCTTGCTA ACCTTATAAT GAACGAAATT
    GTGGACAATG GAACAGCTGT TAAATTTGAT GATATAGCTG GGCAAGAATT GGCAAAACAA
    GCCTTGCAAG AAATTGTTAT TCTTCCTTCT CTGAGGCCTG AGTTGTTCAC AGGGCTTAGA
    GCTCCTGCCA GAGGATTGTT ACTCTTTGGT CCACCTGGAA ATGGGAAAAC AATGCTGGCT
    AAAGCAGTAG CTGCAGAATC CAATGCAACC TTCTTTAATA TAAGTGCTGC AAGTTTAACT
    TCAAAATATG TAGGAGAAGG AGAGAAATTG GTGAGAGCTC TTTTTGCTGT GGCTCGAGAA
    CTTCAGCCTT CAATAATTTT TATAGATGAA GTTGATAGCC TTTTGTGTGA AAGAAGAGAA
    GGAGAGCATG ATGCTAGTAG ACGTCTAAAA ACTGAATTTC TAATAGAATT TGATGGTGTA
    CAATCTGCTG GAGATGACAG AGTGCTTGTA ATGGGTGCAA CTAATAGGCC ACAAGAGCTT
    GATGAGGCTG TTCTCAGGCG TTTCATCAAA CGGGTATATG TGTCTTTGCC AAATGAGGAG
    ACACGACTAC TTTTACTTAA AAATCTATTA TGTAAACAAG GAAGCCCATT GACCCAAAAA
    GAACTAGCAC AACTTGCTAG AATGACCGAT GGGTATTCAG GAAGTGATCT AACAGCTTTG
    GCAAAAGATG CAGCATTGGG TCCTATCCGA GAACTGAAAC CAGAACAAGT AAAGAATATG
    TCTGCCAGTG AGATGAGAAA TATTCGATTA TCTGACTTCA CTGAATCCTT GAAAAAGATA
    AAGCGCAGCG TGAGCCCTCA AACCTTAGAA GCATACATAC GTTGGAACAA GGACTTTGGC
    GATACCACTG TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe18861
    Clone ID Related Accession (Same CDS sequence) XM_005630315.2 , XM_005630315.3
    Accession Version XM_005630315.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1872bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product spastin isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005630315.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAGTTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGGCGG CGGCAGCAGC 
    GGCAGCAGCG GCAGCAGCCC GGTGCCTCCC AGGCCTCCGC CCCCCTGCCT GGCGCCCGCG
    CGTCCCGCCC CCAGGCCGGC CCCCCCGCCC GAGTCTCCGC ATAAGCGGAA CCTGTACTAC
    TTCTCCTACC CGCTGTTCCT AGGCTTCGCG CTGCTGCGTC TGGTCGCCTT CCACCTGGGG
    CTCCTCTTCG TGTGGCTCTG CCAGCGCTTC TCCCGCGCCC TCATGGCTGC CAAGAGGAGC
    TCCCGGGCCG CGCCGGCGCC GGCCTCGGCC TCGCCCCCCG CGCCGGTGCC GGGCGGGGAG
    GCCGAGCGCG TCCGAGCCTT CCACAAGCAG GCCTTCGAGT ACATCTCCGT TGCCCTGCGC
    ATCGATGAGG ACGAGAAAGG ACAAAAAGAG CAAGCTGTGG AATGGTATAA GAAAGGTATT
    GAAGAACTAG AAAAAGGAAT AGCTGTCATA GTTACAGGAC AAGGTGAACA GTATGAAAGA
    GCTAGACGCC TTCAAGCTAA AATGATGACT AATTTGGTTA TGGCCAAGGA CCGTTTACAA
    CTTCTAGAGA AGCTGCAACC AGTTTTGCAA TTTTCCAAGT CACAAACGGA TGTCTATAAT
    GACAGTACTA ACTTGACATG CCGAAACGGA CATCTCCAGT CAGAAAGTGG AGCTGTTCCA
    AAGAGAAAAG ATCCCTTAAC ACACACTAGT AATTCACTGC CTCGTTCAAA AACAGTTATG
    AAGACTGGAT CCACAGGTTT GTCAGGCCAC CACAGAGCAC CTAGTTGTAG TGGCTTATCC
    ATGGTTTCTG GAGTGAGACA GGGACCTGGT CCTGTAACTG GAACTCATAA GAGTACGCCA
    AAAACAAATA GAACAAATAA GCCTTCTACC CCTACAACTG CTGCTCGTAA AAAGAAAGAC
    TTGAAGAATT TTAGGAATGT GGACAGCAAT CTTGCTAACC TTATAATGAA CGAAATTGTG
    GACAATGGAA CAGCTGTTAA ATTTGATGAT ATAGCTGGGC AAGAATTGGC AAAACAAGCC
    TTGCAAGAAA TTGTTATTCT TCCTTCTCTG AGGCCTGAGT TGTTCACAGG GCTTAGAGCT
    CCTGCCAGAG GATTGTTACT CTTTGGTCCA CCTGGAAATG GGAAAACAAT GCTGGCTAAA
    GCAGTAGCTG CAGAATCCAA TGCAACCTTC TTTAATATAA GTGCTGCAAG TTTAACTTCA
    AAATATGTAG GAGAAGGAGA GAAATTGGTG AGAGCTCTTT TTGCTGTGGC TCGAGAACTT
    CAGCCTTCAA TAATTTTTAT AGATGAAGTT GATAGCCTTT TGTGTGAAAG AAGAGAAGGA
    GAGCATGATG CTAGTAGACG TCTAAAAACT GAATTTCTAA TAGAATTTGA TGGTGTACAA
    TCTGCTGGAG ATGACAGAGT GCTTGTAATG GGTGCAACTA ATAGGCCACA AGAGCTTGAT
    GAGGCTGTTC TCAGGCGTTT CATCAAACGG GTATATGTGT CTTTGCCAAA TGAGGAGACA
    CGACTACTTT TACTTAAAAA TCTATTATGT AAACAAGGAA GCCCATTGAC CCAAAAAGAA
    CTAGCACAAC TTGCTAGAAT GACCGATGGG TATTCAGGAA GTGATCTAAC AGCTTTGGCA
    AAAGATGCAG CATTGGGTCC TATCCGAGAA CTGAAACCAG AACAAGTAAA GAATATGTCT
    GCCAGTGAGA TGAGAAATAT TCGATTATCT GACTTCACTG AATCCTTGAA AAAGATAAAG
    CGCAGCGTGA GCCCTCAAAC CTTAGAAGCA TACATACGTT GGAACAAGGA CTTTGGCGAT
    ACCACTGTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005630372.1
    CDS263..2134
    Translation

    Target ORF information:

    RefSeq Version XM_005630315.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris spastin (SPAST), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005630315.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAGTTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGGCGG CGGCAGCAGC 
    GGCAGCAGCG GCAGCAGCCC GGTGCCTCCC AGGCCTCCGC CCCCCTGCCT GGCGCCCGCG
    CGTCCCGCCC CCAGGCCGGC CCCCCCGCCC GAGTCTCCGC ATAAGCGGAA CCTGTACTAC
    TTCTCCTACC CGCTGTTCCT AGGCTTCGCG CTGCTGCGTC TGGTCGCCTT CCACCTGGGG
    CTCCTCTTCG TGTGGCTCTG CCAGCGCTTC TCCCGCGCCC TCATGGCTGC CAAGAGGAGC
    TCCCGGGCCG CGCCGGCGCC GGCCTCGGCC TCGCCCCCCG CGCCGGTGCC GGGCGGGGAG
    GCCGAGCGCG TCCGAGCCTT CCACAAGCAG GCCTTCGAGT ACATCTCCGT TGCCCTGCGC
    ATCGATGAGG ACGAGAAAGG ACAAAAAGAG CAAGCTGTGG AATGGTATAA GAAAGGTATT
    GAAGAACTAG AAAAAGGAAT AGCTGTCATA GTTACAGGAC AAGGTGAACA GTATGAAAGA
    GCTAGACGCC TTCAAGCTAA AATGATGACT AATTTGGTTA TGGCCAAGGA CCGTTTACAA
    CTTCTAGAGA AGCTGCAACC AGTTTTGCAA TTTTCCAAGT CACAAACGGA TGTCTATAAT
    GACAGTACTA ACTTGACATG CCGAAACGGA CATCTCCAGT CAGAAAGTGG AGCTGTTCCA
    AAGAGAAAAG ATCCCTTAAC ACACACTAGT AATTCACTGC CTCGTTCAAA AACAGTTATG
    AAGACTGGAT CCACAGGTTT GTCAGGCCAC CACAGAGCAC CTAGTTGTAG TGGCTTATCC
    ATGGTTTCTG GAGTGAGACA GGGACCTGGT CCTGTAACTG GAACTCATAA GAGTACGCCA
    AAAACAAATA GAACAAATAA GCCTTCTACC CCTACAACTG CTGCTCGTAA AAAGAAAGAC
    TTGAAGAATT TTAGGAATGT GGACAGCAAT CTTGCTAACC TTATAATGAA CGAAATTGTG
    GACAATGGAA CAGCTGTTAA ATTTGATGAT ATAGCTGGGC AAGAATTGGC AAAACAAGCC
    TTGCAAGAAA TTGTTATTCT TCCTTCTCTG AGGCCTGAGT TGTTCACAGG GCTTAGAGCT
    CCTGCCAGAG GATTGTTACT CTTTGGTCCA CCTGGAAATG GGAAAACAAT GCTGGCTAAA
    GCAGTAGCTG CAGAATCCAA TGCAACCTTC TTTAATATAA GTGCTGCAAG TTTAACTTCA
    AAATATGTAG GAGAAGGAGA GAAATTGGTG AGAGCTCTTT TTGCTGTGGC TCGAGAACTT
    CAGCCTTCAA TAATTTTTAT AGATGAAGTT GATAGCCTTT TGTGTGAAAG AAGAGAAGGA
    GAGCATGATG CTAGTAGACG TCTAAAAACT GAATTTCTAA TAGAATTTGA TGGTGTACAA
    TCTGCTGGAG ATGACAGAGT GCTTGTAATG GGTGCAACTA ATAGGCCACA AGAGCTTGAT
    GAGGCTGTTC TCAGGCGTTT CATCAAACGG GTATATGTGT CTTTGCCAAA TGAGGAGACA
    CGACTACTTT TACTTAAAAA TCTATTATGT AAACAAGGAA GCCCATTGAC CCAAAAAGAA
    CTAGCACAAC TTGCTAGAAT GACCGATGGG TATTCAGGAA GTGATCTAAC AGCTTTGGCA
    AAAGATGCAG CATTGGGTCC TATCCGAGAA CTGAAACCAG AACAAGTAAA GAATATGTCT
    GCCAGTGAGA TGAGAAATAT TCGATTATCT GACTTCACTG AATCCTTGAA AAAGATAAAG
    CGCAGCGTGA GCCCTCAAAC CTTAGAAGCA TACATACGTT GGAACAAGGA CTTTGGCGAT
    ACCACTGTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe18861
    Clone ID Related Accession (Same CDS sequence) XM_005630315.2 , XM_005630315.3
    Accession Version XM_005630315.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1872bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product spastin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005630315.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAGTTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGGCGG CGGCAGCAGC 
    GGCAGCAGCG GCAGCAGCCC GGTGCCTCCC AGGCCTCCGC CCCCCTGCCT GGCGCCCGCG
    CGTCCCGCCC CCAGGCCGGC CCCCCCGCCC GAGTCTCCGC ATAAGCGGAA CCTGTACTAC
    TTCTCCTACC CGCTGTTCCT AGGCTTCGCG CTGCTGCGTC TGGTCGCCTT CCACCTGGGG
    CTCCTCTTCG TGTGGCTCTG CCAGCGCTTC TCCCGCGCCC TCATGGCTGC CAAGAGGAGC
    TCCCGGGCCG CGCCGGCGCC GGCCTCGGCC TCGCCCCCCG CGCCGGTGCC GGGCGGGGAG
    GCCGAGCGCG TCCGAGCCTT CCACAAGCAG GCCTTCGAGT ACATCTCCGT TGCCCTGCGC
    ATCGATGAGG ACGAGAAAGG ACAAAAAGAG CAAGCTGTGG AATGGTATAA GAAAGGTATT
    GAAGAACTAG AAAAAGGAAT AGCTGTCATA GTTACAGGAC AAGGTGAACA GTATGAAAGA
    GCTAGACGCC TTCAAGCTAA AATGATGACT AATTTGGTTA TGGCCAAGGA CCGTTTACAA
    CTTCTAGAGA AGCTGCAACC AGTTTTGCAA TTTTCCAAGT CACAAACGGA TGTCTATAAT
    GACAGTACTA ACTTGACATG CCGAAACGGA CATCTCCAGT CAGAAAGTGG AGCTGTTCCA
    AAGAGAAAAG ATCCCTTAAC ACACACTAGT AATTCACTGC CTCGTTCAAA AACAGTTATG
    AAGACTGGAT CCACAGGTTT GTCAGGCCAC CACAGAGCAC CTAGTTGTAG TGGCTTATCC
    ATGGTTTCTG GAGTGAGACA GGGACCTGGT CCTGTAACTG GAACTCATAA GAGTACGCCA
    AAAACAAATA GAACAAATAA GCCTTCTACC CCTACAACTG CTGCTCGTAA AAAGAAAGAC
    TTGAAGAATT TTAGGAATGT GGACAGCAAT CTTGCTAACC TTATAATGAA CGAAATTGTG
    GACAATGGAA CAGCTGTTAA ATTTGATGAT ATAGCTGGGC AAGAATTGGC AAAACAAGCC
    TTGCAAGAAA TTGTTATTCT TCCTTCTCTG AGGCCTGAGT TGTTCACAGG GCTTAGAGCT
    CCTGCCAGAG GATTGTTACT CTTTGGTCCA CCTGGAAATG GGAAAACAAT GCTGGCTAAA
    GCAGTAGCTG CAGAATCCAA TGCAACCTTC TTTAATATAA GTGCTGCAAG TTTAACTTCA
    AAATATGTAG GAGAAGGAGA GAAATTGGTG AGAGCTCTTT TTGCTGTGGC TCGAGAACTT
    CAGCCTTCAA TAATTTTTAT AGATGAAGTT GATAGCCTTT TGTGTGAAAG AAGAGAAGGA
    GAGCATGATG CTAGTAGACG TCTAAAAACT GAATTTCTAA TAGAATTTGA TGGTGTACAA
    TCTGCTGGAG ATGACAGAGT GCTTGTAATG GGTGCAACTA ATAGGCCACA AGAGCTTGAT
    GAGGCTGTTC TCAGGCGTTT CATCAAACGG GTATATGTGT CTTTGCCAAA TGAGGAGACA
    CGACTACTTT TACTTAAAAA TCTATTATGT AAACAAGGAA GCCCATTGAC CCAAAAAGAA
    CTAGCACAAC TTGCTAGAAT GACCGATGGG TATTCAGGAA GTGATCTAAC AGCTTTGGCA
    AAAGATGCAG CATTGGGTCC TATCCGAGAA CTGAAACCAG AACAAGTAAA GAATATGTCT
    GCCAGTGAGA TGAGAAATAT TCGATTATCT GACTTCACTG AATCCTTGAA AAAGATAAAG
    CGCAGCGTGA GCCCTCAAAC CTTAGAAGCA TACATACGTT GGAACAAGGA CTTTGGCGAT
    ACCACTGTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005630372.1
    CDS253..2124
    Translation

    Target ORF information:

    RefSeq Version XM_005630315.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris spastin (SPAST), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005630315.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAGTTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGGCGG CGGCAGCAGC 
    GGCAGCAGCG GCAGCAGCCC GGTGCCTCCC AGGCCTCCGC CCCCCTGCCT GGCGCCCGCG
    CGTCCCGCCC CCAGGCCGGC CCCCCCGCCC GAGTCTCCGC ATAAGCGGAA CCTGTACTAC
    TTCTCCTACC CGCTGTTCCT AGGCTTCGCG CTGCTGCGTC TGGTCGCCTT CCACCTGGGG
    CTCCTCTTCG TGTGGCTCTG CCAGCGCTTC TCCCGCGCCC TCATGGCTGC CAAGAGGAGC
    TCCCGGGCCG CGCCGGCGCC GGCCTCGGCC TCGCCCCCCG CGCCGGTGCC GGGCGGGGAG
    GCCGAGCGCG TCCGAGCCTT CCACAAGCAG GCCTTCGAGT ACATCTCCGT TGCCCTGCGC
    ATCGATGAGG ACGAGAAAGG ACAAAAAGAG CAAGCTGTGG AATGGTATAA GAAAGGTATT
    GAAGAACTAG AAAAAGGAAT AGCTGTCATA GTTACAGGAC AAGGTGAACA GTATGAAAGA
    GCTAGACGCC TTCAAGCTAA AATGATGACT AATTTGGTTA TGGCCAAGGA CCGTTTACAA
    CTTCTAGAGA AGCTGCAACC AGTTTTGCAA TTTTCCAAGT CACAAACGGA TGTCTATAAT
    GACAGTACTA ACTTGACATG CCGAAACGGA CATCTCCAGT CAGAAAGTGG AGCTGTTCCA
    AAGAGAAAAG ATCCCTTAAC ACACACTAGT AATTCACTGC CTCGTTCAAA AACAGTTATG
    AAGACTGGAT CCACAGGTTT GTCAGGCCAC CACAGAGCAC CTAGTTGTAG TGGCTTATCC
    ATGGTTTCTG GAGTGAGACA GGGACCTGGT CCTGTAACTG GAACTCATAA GAGTACGCCA
    AAAACAAATA GAACAAATAA GCCTTCTACC CCTACAACTG CTGCTCGTAA AAAGAAAGAC
    TTGAAGAATT TTAGGAATGT GGACAGCAAT CTTGCTAACC TTATAATGAA CGAAATTGTG
    GACAATGGAA CAGCTGTTAA ATTTGATGAT ATAGCTGGGC AAGAATTGGC AAAACAAGCC
    TTGCAAGAAA TTGTTATTCT TCCTTCTCTG AGGCCTGAGT TGTTCACAGG GCTTAGAGCT
    CCTGCCAGAG GATTGTTACT CTTTGGTCCA CCTGGAAATG GGAAAACAAT GCTGGCTAAA
    GCAGTAGCTG CAGAATCCAA TGCAACCTTC TTTAATATAA GTGCTGCAAG TTTAACTTCA
    AAATATGTAG GAGAAGGAGA GAAATTGGTG AGAGCTCTTT TTGCTGTGGC TCGAGAACTT
    CAGCCTTCAA TAATTTTTAT AGATGAAGTT GATAGCCTTT TGTGTGAAAG AAGAGAAGGA
    GAGCATGATG CTAGTAGACG TCTAAAAACT GAATTTCTAA TAGAATTTGA TGGTGTACAA
    TCTGCTGGAG ATGACAGAGT GCTTGTAATG GGTGCAACTA ATAGGCCACA AGAGCTTGAT
    GAGGCTGTTC TCAGGCGTTT CATCAAACGG GTATATGTGT CTTTGCCAAA TGAGGAGACA
    CGACTACTTT TACTTAAAAA TCTATTATGT AAACAAGGAA GCCCATTGAC CCAAAAAGAA
    CTAGCACAAC TTGCTAGAAT GACCGATGGG TATTCAGGAA GTGATCTAAC AGCTTTGGCA
    AAAGATGCAG CATTGGGTCC TATCCGAGAA CTGAAACCAG AACAAGTAAA GAATATGTCT
    GCCAGTGAGA TGAGAAATAT TCGATTATCT GACTTCACTG AATCCTTGAA AAAGATAAAG
    CGCAGCGTGA GCCCTCAAAC CTTAGAAGCA TACATACGTT GGAACAAGGA CTTTGGCGAT
    ACCACTGTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe18862
    Clone ID Related Accession (Same CDS sequence) XM_857738.4 , XM_857738.5
    Accession Version XM_857738.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1779bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product spastin isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_857738.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAGTTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGGCGG CGGCAGCAGC 
    GGCAGCAGCG GCAGCAGCCC GGTGCCTCCC AGGCCTCCGC CCCCCTGCCT GGCGCCCGCG
    CGTCCCGCCC CCAGGCCGGC CCCCCCGCCC GAGTCTCCGC ATAAGCGGAA CCTGTACTAC
    TTCTCCTACC CGCTGTTCCT AGGCTTCGCG CTGCTGCGTC TGGTCGCCTT CCACCTGGGG
    CTCCTCTTCG TGTGGCTCTG CCAGCGCTTC TCCCGCGCCC TCATGGCTGC CAAGAGGAGC
    TCCCGGGCCG CGCCGGCGCC GGCCTCGGCC TCGCCCCCCG CGCCGGTGCC GGGCGGGGAG
    GCCGAGCGCG TCCGAGCCTT CCACAAGCAG GCCTTCGAGT ACATCTCCGT TGCCCTGCGC
    ATCGATGAGG ACGAGAAAGC AGGACAAAAA GAGCAAGCTG TGGAATGGTA TAAGAAAGGT
    ATTGAAGAAC TAGAAAAAGG AATAGCTGTC ATAGTTACAG GACAAGGTGA ACAGTATGAA
    AGAGCTAGAC GCCTTCAAGC TAAAATGATG ACTAATTTGG TTATGGCCAA GGACCGTTTA
    CAACTTCTAG AAAGTGGAGC TGTTCCAAAG AGAAAAGATC CCTTAACACA CACTAGTAAT
    TCACTGCCTC GTTCAAAAAC AGTTATGAAG ACTGGATCCA CAGGTTTGTC AGGCCACCAC
    AGAGCACCTA GTTGTAGTGG CTTATCCATG GTTTCTGGAG TGAGACAGGG ACCTGGTCCT
    GTAACTGGAA CTCATAAGAG TACGCCAAAA ACAAATAGAA CAAATAAGCC TTCTACCCCT
    ACAACTGCTG CTCGTAAAAA GAAAGACTTG AAGAATTTTA GGAATGTGGA CAGCAATCTT
    GCTAACCTTA TAATGAACGA AATTGTGGAC AATGGAACAG CTGTTAAATT TGATGATATA
    GCTGGGCAAG AATTGGCAAA ACAAGCCTTG CAAGAAATTG TTATTCTTCC TTCTCTGAGG
    CCTGAGTTGT TCACAGGGCT TAGAGCTCCT GCCAGAGGAT TGTTACTCTT TGGTCCACCT
    GGAAATGGGA AAACAATGCT GGCTAAAGCA GTAGCTGCAG AATCCAATGC AACCTTCTTT
    AATATAAGTG CTGCAAGTTT AACTTCAAAA TATGTAGGAG AAGGAGAGAA ATTGGTGAGA
    GCTCTTTTTG CTGTGGCTCG AGAACTTCAG CCTTCAATAA TTTTTATAGA TGAAGTTGAT
    AGCCTTTTGT GTGAAAGAAG AGAAGGAGAG CATGATGCTA GTAGACGTCT AAAAACTGAA
    TTTCTAATAG AATTTGATGG TGTACAATCT GCTGGAGATG ACAGAGTGCT TGTAATGGGT
    GCAACTAATA GGCCACAAGA GCTTGATGAG GCTGTTCTCA GGCGTTTCAT CAAACGGGTA
    TATGTGTCTT TGCCAAATGA GGAGACACGA CTACTTTTAC TTAAAAATCT ATTATGTAAA
    CAAGGAAGCC CATTGACCCA AAAAGAACTA GCACAACTTG CTAGAATGAC CGATGGGTAT
    TCAGGAAGTG ATCTAACAGC TTTGGCAAAA GATGCAGCAT TGGGTCCTAT CCGAGAACTG
    AAACCAGAAC AAGTAAAGAA TATGTCTGCC AGTGAGATGA GAAATATTCG ATTATCTGAC
    TTCACTGAAT CCTTGAAAAA GATAAAGCGC AGCGTGAGCC CTCAAACCTT AGAAGCATAC
    ATACGTTGGA ACAAGGACTT TGGCGATACC ACTGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_862831.1
    CDS264..2042
    Translation

    Target ORF information:

    RefSeq Version XM_857738.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris spastin (SPAST), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_857738.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAGTTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGGCGG CGGCAGCAGC 
    GGCAGCAGCG GCAGCAGCCC GGTGCCTCCC AGGCCTCCGC CCCCCTGCCT GGCGCCCGCG
    CGTCCCGCCC CCAGGCCGGC CCCCCCGCCC GAGTCTCCGC ATAAGCGGAA CCTGTACTAC
    TTCTCCTACC CGCTGTTCCT AGGCTTCGCG CTGCTGCGTC TGGTCGCCTT CCACCTGGGG
    CTCCTCTTCG TGTGGCTCTG CCAGCGCTTC TCCCGCGCCC TCATGGCTGC CAAGAGGAGC
    TCCCGGGCCG CGCCGGCGCC GGCCTCGGCC TCGCCCCCCG CGCCGGTGCC GGGCGGGGAG
    GCCGAGCGCG TCCGAGCCTT CCACAAGCAG GCCTTCGAGT ACATCTCCGT TGCCCTGCGC
    ATCGATGAGG ACGAGAAAGC AGGACAAAAA GAGCAAGCTG TGGAATGGTA TAAGAAAGGT
    ATTGAAGAAC TAGAAAAAGG AATAGCTGTC ATAGTTACAG GACAAGGTGA ACAGTATGAA
    AGAGCTAGAC GCCTTCAAGC TAAAATGATG ACTAATTTGG TTATGGCCAA GGACCGTTTA
    CAACTTCTAG AAAGTGGAGC TGTTCCAAAG AGAAAAGATC CCTTAACACA CACTAGTAAT
    TCACTGCCTC GTTCAAAAAC AGTTATGAAG ACTGGATCCA CAGGTTTGTC AGGCCACCAC
    AGAGCACCTA GTTGTAGTGG CTTATCCATG GTTTCTGGAG TGAGACAGGG ACCTGGTCCT
    GTAACTGGAA CTCATAAGAG TACGCCAAAA ACAAATAGAA CAAATAAGCC TTCTACCCCT
    ACAACTGCTG CTCGTAAAAA GAAAGACTTG AAGAATTTTA GGAATGTGGA CAGCAATCTT
    GCTAACCTTA TAATGAACGA AATTGTGGAC AATGGAACAG CTGTTAAATT TGATGATATA
    GCTGGGCAAG AATTGGCAAA ACAAGCCTTG CAAGAAATTG TTATTCTTCC TTCTCTGAGG
    CCTGAGTTGT TCACAGGGCT TAGAGCTCCT GCCAGAGGAT TGTTACTCTT TGGTCCACCT
    GGAAATGGGA AAACAATGCT GGCTAAAGCA GTAGCTGCAG AATCCAATGC AACCTTCTTT
    AATATAAGTG CTGCAAGTTT AACTTCAAAA TATGTAGGAG AAGGAGAGAA ATTGGTGAGA
    GCTCTTTTTG CTGTGGCTCG AGAACTTCAG CCTTCAATAA TTTTTATAGA TGAAGTTGAT
    AGCCTTTTGT GTGAAAGAAG AGAAGGAGAG CATGATGCTA GTAGACGTCT AAAAACTGAA
    TTTCTAATAG AATTTGATGG TGTACAATCT GCTGGAGATG ACAGAGTGCT TGTAATGGGT
    GCAACTAATA GGCCACAAGA GCTTGATGAG GCTGTTCTCA GGCGTTTCAT CAAACGGGTA
    TATGTGTCTT TGCCAAATGA GGAGACACGA CTACTTTTAC TTAAAAATCT ATTATGTAAA
    CAAGGAAGCC CATTGACCCA AAAAGAACTA GCACAACTTG CTAGAATGAC CGATGGGTAT
    TCAGGAAGTG ATCTAACAGC TTTGGCAAAA GATGCAGCAT TGGGTCCTAT CCGAGAACTG
    AAACCAGAAC AAGTAAAGAA TATGTCTGCC AGTGAGATGA GAAATATTCG ATTATCTGAC
    TTCACTGAAT CCTTGAAAAA GATAAAGCGC AGCGTGAGCC CTCAAACCTT AGAAGCATAC
    ATACGTTGGA ACAAGGACTT TGGCGATACC ACTGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe18862
    Clone ID Related Accession (Same CDS sequence) XM_857738.4 , XM_857738.5
    Accession Version XM_857738.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1779bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product spastin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_857738.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAGTTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGGCGG CGGCAGCAGC 
    GGCAGCAGCG GCAGCAGCCC GGTGCCTCCC AGGCCTCCGC CCCCCTGCCT GGCGCCCGCG
    CGTCCCGCCC CCAGGCCGGC CCCCCCGCCC GAGTCTCCGC ATAAGCGGAA CCTGTACTAC
    TTCTCCTACC CGCTGTTCCT AGGCTTCGCG CTGCTGCGTC TGGTCGCCTT CCACCTGGGG
    CTCCTCTTCG TGTGGCTCTG CCAGCGCTTC TCCCGCGCCC TCATGGCTGC CAAGAGGAGC
    TCCCGGGCCG CGCCGGCGCC GGCCTCGGCC TCGCCCCCCG CGCCGGTGCC GGGCGGGGAG
    GCCGAGCGCG TCCGAGCCTT CCACAAGCAG GCCTTCGAGT ACATCTCCGT TGCCCTGCGC
    ATCGATGAGG ACGAGAAAGC AGGACAAAAA GAGCAAGCTG TGGAATGGTA TAAGAAAGGT
    ATTGAAGAAC TAGAAAAAGG AATAGCTGTC ATAGTTACAG GACAAGGTGA ACAGTATGAA
    AGAGCTAGAC GCCTTCAAGC TAAAATGATG ACTAATTTGG TTATGGCCAA GGACCGTTTA
    CAACTTCTAG AAAGTGGAGC TGTTCCAAAG AGAAAAGATC CCTTAACACA CACTAGTAAT
    TCACTGCCTC GTTCAAAAAC AGTTATGAAG ACTGGATCCA CAGGTTTGTC AGGCCACCAC
    AGAGCACCTA GTTGTAGTGG CTTATCCATG GTTTCTGGAG TGAGACAGGG ACCTGGTCCT
    GTAACTGGAA CTCATAAGAG TACGCCAAAA ACAAATAGAA CAAATAAGCC TTCTACCCCT
    ACAACTGCTG CTCGTAAAAA GAAAGACTTG AAGAATTTTA GGAATGTGGA CAGCAATCTT
    GCTAACCTTA TAATGAACGA AATTGTGGAC AATGGAACAG CTGTTAAATT TGATGATATA
    GCTGGGCAAG AATTGGCAAA ACAAGCCTTG CAAGAAATTG TTATTCTTCC TTCTCTGAGG
    CCTGAGTTGT TCACAGGGCT TAGAGCTCCT GCCAGAGGAT TGTTACTCTT TGGTCCACCT
    GGAAATGGGA AAACAATGCT GGCTAAAGCA GTAGCTGCAG AATCCAATGC AACCTTCTTT
    AATATAAGTG CTGCAAGTTT AACTTCAAAA TATGTAGGAG AAGGAGAGAA ATTGGTGAGA
    GCTCTTTTTG CTGTGGCTCG AGAACTTCAG CCTTCAATAA TTTTTATAGA TGAAGTTGAT
    AGCCTTTTGT GTGAAAGAAG AGAAGGAGAG CATGATGCTA GTAGACGTCT AAAAACTGAA
    TTTCTAATAG AATTTGATGG TGTACAATCT GCTGGAGATG ACAGAGTGCT TGTAATGGGT
    GCAACTAATA GGCCACAAGA GCTTGATGAG GCTGTTCTCA GGCGTTTCAT CAAACGGGTA
    TATGTGTCTT TGCCAAATGA GGAGACACGA CTACTTTTAC TTAAAAATCT ATTATGTAAA
    CAAGGAAGCC CATTGACCCA AAAAGAACTA GCACAACTTG CTAGAATGAC CGATGGGTAT
    TCAGGAAGTG ATCTAACAGC TTTGGCAAAA GATGCAGCAT TGGGTCCTAT CCGAGAACTG
    AAACCAGAAC AAGTAAAGAA TATGTCTGCC AGTGAGATGA GAAATATTCG ATTATCTGAC
    TTCACTGAAT CCTTGAAAAA GATAAAGCGC AGCGTGAGCC CTCAAACCTT AGAAGCATAC
    ATACGTTGGA ACAAGGACTT TGGCGATACC ACTGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_862831.1
    CDS254..2032
    Translation

    Target ORF information:

    RefSeq Version XM_857738.5
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris spastin (SPAST), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_857738.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAGTTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGGCGG CGGCAGCAGC 
    GGCAGCAGCG GCAGCAGCCC GGTGCCTCCC AGGCCTCCGC CCCCCTGCCT GGCGCCCGCG
    CGTCCCGCCC CCAGGCCGGC CCCCCCGCCC GAGTCTCCGC ATAAGCGGAA CCTGTACTAC
    TTCTCCTACC CGCTGTTCCT AGGCTTCGCG CTGCTGCGTC TGGTCGCCTT CCACCTGGGG
    CTCCTCTTCG TGTGGCTCTG CCAGCGCTTC TCCCGCGCCC TCATGGCTGC CAAGAGGAGC
    TCCCGGGCCG CGCCGGCGCC GGCCTCGGCC TCGCCCCCCG CGCCGGTGCC GGGCGGGGAG
    GCCGAGCGCG TCCGAGCCTT CCACAAGCAG GCCTTCGAGT ACATCTCCGT TGCCCTGCGC
    ATCGATGAGG ACGAGAAAGC AGGACAAAAA GAGCAAGCTG TGGAATGGTA TAAGAAAGGT
    ATTGAAGAAC TAGAAAAAGG AATAGCTGTC ATAGTTACAG GACAAGGTGA ACAGTATGAA
    AGAGCTAGAC GCCTTCAAGC TAAAATGATG ACTAATTTGG TTATGGCCAA GGACCGTTTA
    CAACTTCTAG AAAGTGGAGC TGTTCCAAAG AGAAAAGATC CCTTAACACA CACTAGTAAT
    TCACTGCCTC GTTCAAAAAC AGTTATGAAG ACTGGATCCA CAGGTTTGTC AGGCCACCAC
    AGAGCACCTA GTTGTAGTGG CTTATCCATG GTTTCTGGAG TGAGACAGGG ACCTGGTCCT
    GTAACTGGAA CTCATAAGAG TACGCCAAAA ACAAATAGAA CAAATAAGCC TTCTACCCCT
    ACAACTGCTG CTCGTAAAAA GAAAGACTTG AAGAATTTTA GGAATGTGGA CAGCAATCTT
    GCTAACCTTA TAATGAACGA AATTGTGGAC AATGGAACAG CTGTTAAATT TGATGATATA
    GCTGGGCAAG AATTGGCAAA ACAAGCCTTG CAAGAAATTG TTATTCTTCC TTCTCTGAGG
    CCTGAGTTGT TCACAGGGCT TAGAGCTCCT GCCAGAGGAT TGTTACTCTT TGGTCCACCT
    GGAAATGGGA AAACAATGCT GGCTAAAGCA GTAGCTGCAG AATCCAATGC AACCTTCTTT
    AATATAAGTG CTGCAAGTTT AACTTCAAAA TATGTAGGAG AAGGAGAGAA ATTGGTGAGA
    GCTCTTTTTG CTGTGGCTCG AGAACTTCAG CCTTCAATAA TTTTTATAGA TGAAGTTGAT
    AGCCTTTTGT GTGAAAGAAG AGAAGGAGAG CATGATGCTA GTAGACGTCT AAAAACTGAA
    TTTCTAATAG AATTTGATGG TGTACAATCT GCTGGAGATG ACAGAGTGCT TGTAATGGGT
    GCAACTAATA GGCCACAAGA GCTTGATGAG GCTGTTCTCA GGCGTTTCAT CAAACGGGTA
    TATGTGTCTT TGCCAAATGA GGAGACACGA CTACTTTTAC TTAAAAATCT ATTATGTAAA
    CAAGGAAGCC CATTGACCCA AAAAGAACTA GCACAACTTG CTAGAATGAC CGATGGGTAT
    TCAGGAAGTG ATCTAACAGC TTTGGCAAAA GATGCAGCAT TGGGTCCTAT CCGAGAACTG
    AAACCAGAAC AAGTAAAGAA TATGTCTGCC AGTGAGATGA GAAATATTCG ATTATCTGAC
    TTCACTGAAT CCTTGAAAAA GATAAAGCGC AGCGTGAGCC CTCAAACCTT AGAAGCATAC
    ATACGTTGGA ACAAGGACTT TGGCGATACC ACTGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.