MYOF cDNA ORF clone, Canis lupus familiaris(dog)

The following MYOF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYOF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe283361 XM_022412118.1
Latest version!
Canis lupus familiaris myoferlin (MYOF), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe283360 XM_022412117.1
Latest version!
Canis lupus familiaris myoferlin (MYOF), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OCe27786 XM_853765.5
Latest version!
Canis lupus familiaris myoferlin (MYOF), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe27787 XM_005637544.3
Latest version!
Canis lupus familiaris myoferlin (MYOF), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
View Old Accession Versions >>
OCe27787 XM_005637544.2 Canis lupus familiaris myoferlin (MYOF), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe27786 XM_853765.4 Canis lupus familiaris myoferlin (MYOF), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe283361
    Clone ID Related Accession (Same CDS sequence) XM_022412118.1
    Accession Version XM_022412118.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5166bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product myoferlin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006610.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGGG TGATCGTGGA GTCCGCCAGC AATATCCCGA AAACGAAATT TGGGAAACCG 
    GATCCTATCG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGACAAC
    GAACTCAATC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGAGGGGTAT ACCACTGGAC
    TTTTCATCTT CCCTTACAAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAATTA
    ATTGGCACGG CCACTGTAGC CCTGAAGGAC CTGACTGGTG ACCAGAGCAG ATCACTGCCG
    TACAAACTCA TCTCCCTGCT CAGCGAAAAG GGGCAAGATA CTGGGGCCAC CATTGACTTG
    GTGATTGGCT ATGATCCACC TTCTGCTCCA CATCCAAATG ACCCCAGTGG GACCAGTGTG
    TCAGTCACAG AAGGAGAAGA AGAGGAAGAT GGAGGTGATG AAGACAGGTT GGATGGCACA
    GTCACGGGCC CTGGGCCCAG GGGGTCAGGT GGGATCGTGT CGGAGGCTCA CCTTGCCCGG
    AGGCTCACGA AAGCAAAGAA GAACCGGCGG ATGCTGTCCA ACAAGCCTCA GGACTTCCAG
    ATCCGCGTCC GAGTGATTGA GGGCCGTCAG TTAAGTGGCA ACAACATCAA GCCTGTGGTC
    AAGGTCCATG TCTGTGGCCA GACACACAGA ACAAGGATCA AGAGAGGGAA CAACCCATTT
    TATGATGAGT TATTTTTCTA CAATGTCCAC ATAACCCCTT CTGAACTGAT GGATGAGATC
    ATCAGCATTC GGGTTTATAA TTCCCATTCT TTGCGGGCAG ATTGTCTGAT GGGGGAATTT
    AAGATTGATG TTGGATTCAT TTATGATGAA CCGGGTCACG CTGTCATGAG AAAATGGCTT
    CTTCTCAATG ACCCTGAAGA TGCCAGCTCA GGTGCTAAAG GTTACATGAA AGTCAGCATG
    TTCATCCTGG GAACTGGAGA TGAGCCTCCT GCTGAGAAGC GAGATCGTGA TAATGACAGT
    GATGACGTGG AGAGTAATTT GTTACTCCCT GCTGGCATCG CCCTCCGGTG GGTGACCTTC
    TTGCTTAAAA TCTACCGAGC AGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA
    GTAAAGGAAA TATTTGGAGG CAATGCAGAT AAAAAAAACC TAGTGGATCC TTTTGTAGAA
    GTTTCCTTTG CTGGAAAAAA GGTTTGCACA AATATAATTG AGAAAAATGC AAATCCTGAG
    TGGAACCAAG TCGTTAATCT TCAGATAAAG TTTCCTTCAA TGTGTGAAAA AATAAAACTA
    ACAATATATG ACTGGGACCG TCTTACTAAA AATGATGTTG TTGGAACAAC ATATCTACAC
    CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGATC TCTCATCTTC GGGATCTGGG
    GCTGCGTCAT ATACAGCAAA TACAGGAGAA ACAGAGGTGG GCTTTGTTCC AACATTTGGG
    CCTTGTTATC TGAACCTTTA CGGAAGCCCT AGAGAGTACA CAGGATTCCC AGACCCCTAT
    GATGAGCTAA ATACTGGAAA GGGGGAAGGA GTTGCCTACA GAGGCAGAAT CTTGGTTGAA
    TTAAGCACTT TTCTTGAAAA GACACCACCA GACAAAAAGC TTGAGCCTAT TTCAAATGAT
    GACCTGCTGG TTGTGGAGAA ATACCAACGA AGGCGGAAGT ATAGCCTGTC TGCCGTGTTC
    CATTCGGCCA CCATGTTGCA AGATGTTGGC GAGGCCATTC AGTTTGAAGT CAGCATTGGG
    AACTATGGCA ACAAGTTTGA CACGACTTGT AAGCCTTTGG CATCAACCAC TCAATACAGC
    CGTGCCGTAT TTGATGGGAA CTACTATTAT TACTTGCCTT GGGCCCACAC AAAGCCGGTT
    GTTACCCTGA CTTCATACTG GGAGGACATC AGTCATCGCC TGGATGCAGT GAACACTCTC
    CTAACCATGG CAGAACGGCT GCAATTGAAC TTAGAGGCTC TGAAATCAGG GATGCAAGGT
    AAAGTTCCTG CAAACCAGTT GGCTGAAATA TGGTTGAAGC TGATCGATGA AGTTATAGAA
    GATACAAGGT ACACATTGCC TGTCACGGAA GGAAAAGCTA ATGTCACAAT TCTTGACACT
    CAGATCCGAA AGTTGCGGTC CCGATCTCTC TCCCAGATTC ATGAGGCAGC TGTGAGGATG
    AGGTCTGAGG CCACAGAGGT GAAATCCACA CTGGCAGAAA TTGAGGACTG GCTTGAAAAA
    TTACTGCAAC TGACTGAAGA GCCACAGAAC AGTATGCCTG ACATTATCAT CTGGATGATC
    CGGGGGGAGA AAAGACTGGC CTATGCACGC ATCCCTGCCC ATCAGGTTCT ATACTCCACC
    AGTGGTGAGA CTGCATCTGG GAAATACTGC GGGAAAACCC AAACCATCTT GCTGAAGTAT
    CCGCAGGAAA AAACCCACGG ACCAAAGGTG CCCGCAGAGC TGCGAGTCAA CATCTGGCTG
    GGCTTAAGTG CTGTTGAGAA GAAATTCAAT AGCTTCGCAG AAGGAACATT CACCGTCTTT
    GCTGAAATGT ATGAAAATCA AGCTCTTATG TTTGGAAAAT GGGGCACTTC TGGATTAGTG
    GGTCGTCATA AATTTTCTGA TGTCACAGGA AAAATAAAAC TCAAGAGGGA ATTCTTTTTG
    CCCCCGAAAG GCTGGGAATG GGAAGGAGAG TGGATAGTTG ATCCTGAAAG AAGCTTGCTG
    ACTGAAGCAG ACGCAGGGCA CACGGAGTTC ACGGACGAAG TGTACCAGAA TGAGAGCCGC
    TACCCTGGGG GTGACTGGAA GCCAGCTGAG GACACCTATA CCGATGCGAA TGGTGATAAA
    GCGGCATCAC CTAGCGAGCT GACATGCCCT CCAGGATGGG AATGGGAAGA TGACGCATGG
    GTATATGATA TAAATCGTGC AGTGGATGAG AAAGGCTGGG AGTATGGAAT TACCATTCCT
    CCTGATACTA AACCCAAATC CTGGGTTGCA GCAGAGAAAA TGTATCATAC TCATAGACGA
    CGAAGGCTAG TCCGAAAACG CAAGAAAGAT TTAACACAAA GCACTGCAAG CACTGCAAGG
    GCCATGGAAG AATTGGAAGA CCGGGAGGGC TGGGAATATG CTTCTTTAAT TGGCTGGAAA
    TTTCACTGGA AACAGCGTAG CTCAGATACC TTCCGCCGTA GACGCTGGAG AAGGAAAATG
    GCTCCTTCGG AAACGCACGG TGCGGCCGCC ATCTTTAAAC TGGAGGGCGC CCTCGGTGCA
    GACATGACCG AGGATGGAGA CGAGAAGAGT GTGGACAAGC AGAAGTATAG TGCCACCACT
    GTATTTGGGG CAAACACTCC CATTGTTTCC TGTAACTTTG ACAGAGTCTA CATTTACCAT
    CTGCGCTGCT ATATCTATCA AGCCAGAAAC CTCATGGCTC TAGACAAGGA TAGTTTTTCA
    GATCCATATG CACATGTCTC CTTCCTCCAT CGAAGCAAAA CCACTGAGAT CATCCACTCG
    ACTCTGAATC CCACATGGGA CCAGACAATT ATATTTGATG AAATCGAAAT CTATGGGGAA
    CCCCAGACAG TTCTACAGAA CCCACCCAAA GTTATCATCG AACTTTTTGA CAATGACCAA
    GTGGGCAAAG ATGAATTTTT AGGACGAAGC ATGTGCTCTC CTCTGGTAAA ACTAAACTCA
    GAAACGGATG TCACACCCAA ACTTCTCTGG CACCCTGTAA TGAATGGAGA CAAAGCCTGT
    GGGGATGTCC TTGTAACTGC AGAGCTGATT CTAAGGCACA AGGATGGTTC CAATCTTCCC
    ATCCTTCCCT CACAAAGGGC ACCAAATCTA TACATGGTCC CACAAGGGAT CAGGCCTGTG
    GTCCAACTCA CTGCTATTGA GATTCTAGCT TGGGGCTTAA GAAATATGAA AAACTACCAG
    ATGGCTTCTA TCACCTCCCC CAGCCTCATT GTGGAGTGTG GAGGGGAAAG GGTAGAATCA
    GTGGTGATCA AAAACCTGAA GAAGACACCC AACTTCCCAA GTTCTGTTCT CTTCATGAAA
    GTGTTCTTGC CCAAGGAGGA ACTGTACATG CCTCCGCTGG TGATAAAGGT CATCGACCAC
    AGGCAGTTCG GGCGGAAGCC CGTTGTTGGG CAGTGCACCA TCGATCGTCT GGACCGCTTT
    CGTTGTGACC CTTATGCGGG AAAGGAGGAT CTTGTGCCCC AGCTAAAAGC CTCCCTTCTA
    TCTACCCCAC CCTGCCGGGA TGTCATTATC GAAATAGAAG ACACCAAACC ATTACTGGCT
    TCGAAGCTGA CAGAAAAGGA GGAAGAAATC GTTGACTGGT GGAGTAAATT TTATGCTTCC
    ACAGGAGAAC ATGAAAAATG TGGACAATAT GTTCAGAAAG GATATTCGAA ACTCAAGATA
    TATGATTGTG AACTAGAGGA TGTAACAGAA TTTGAAGGCC TGACAGACTT CTCAGATACT
    TTTAAGCTGT ATCGAGGCAA ATCAGATGAA AATGAAGATC CCTCAGTGGT AGGAGAGTTT
    AAGGGCTCCT TTAGAATCTA CTCTCTGCCG GATGACCCCA GCGTGCCGGC CCCTCCCAGG
    CAGTTTCGGG AATTACCTGA CAGCGTCCCA CAGGAATGCA CGGTTAGGAT TTACATTGTT
    CGAGGCTTAG AGCTCCAGCC CCAGGACAAC AATGGCCTGT GTGACCCTTA TATAAAAATA
    ACACTGGGCA AAAAAGTAAT AGAAGATCGA GACCACTATA TCCCCAACAC CCTCAACCCA
    GTTTTCGGCA GAATGTATGA ACTGAGCTGC TACTTACCTC AAGAAAAAGA TCTGAAAATT
    TCTGTCTATG ACTACGACAC CTTTACCCGT GATGAAAAGG TGGGAGAAAC AATTATTGAT
    CTGGAAAACC GATTCCTTTC CCGGTTTGGG TCTCACTGCG GCATACCTGA GCAGTACTGT
    GTTTCTGGAG TTAACACCTG GCGAGATCAA CTGAGACCAA CTCAGTTGCT TCAAAATGTA
    GCCAGGTTCA AAGGCTTCCC ACCACCCATC CTTTCTGAAG ATGGAAATAG AATCAGATAT
    GGAGGGCGAG ACTACAGTTT GGATGAATTT GGGGAAACTT CAGATGTGGG TGGATGTTTT
    CCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022267826.1
    CDS130..5295
    Translation

    Target ORF information:

    RefSeq Version XM_022412118.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris myoferlin (MYOF), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022412118.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGGG TGATCGTGGA GTCCGCCAGC AATATCCCGA AAACGAAATT TGGGAAACCG 
    GATCCTATCG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGACAAC
    GAACTCAATC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGAGGGGTAT ACCACTGGAC
    TTTTCATCTT CCCTTACAAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAATTA
    ATTGGCACGG CCACTGTAGC CCTGAAGGAC CTGACTGGTG ACCAGAGCAG ATCACTGCCG
    TACAAACTCA TCTCCCTGCT CAGCGAAAAG GGGCAAGATA CTGGGGCCAC CATTGACTTG
    GTGATTGGCT ATGATCCACC TTCTGCTCCA CATCCAAATG ACCCCAGTGG GACCAGTGTG
    TCAGTCACAG AAGGAGAAGA AGAGGAAGAT GGAGGTGATG AAGACAGGTT GGATGGCACA
    GTCACGGGCC CTGGGCCCAG GGGGTCAGGT GGGATCGTGT CGGAGGCTCA CCTTGCCCGG
    AGGCTCACGA AAGCAAAGAA GAACCGGCGG ATGCTGTCCA ACAAGCCTCA GGACTTCCAG
    ATCCGCGTCC GAGTGATTGA GGGCCGTCAG TTAAGTGGCA ACAACATCAA GCCTGTGGTC
    AAGGTCCATG TCTGTGGCCA GACACACAGA ACAAGGATCA AGAGAGGGAA CAACCCATTT
    TATGATGAGT TATTTTTCTA CAATGTCCAC ATAACCCCTT CTGAACTGAT GGATGAGATC
    ATCAGCATTC GGGTTTATAA TTCCCATTCT TTGCGGGCAG ATTGTCTGAT GGGGGAATTT
    AAGATTGATG TTGGATTCAT TTATGATGAA CCGGGTCACG CTGTCATGAG AAAATGGCTT
    CTTCTCAATG ACCCTGAAGA TGCCAGCTCA GGTGCTAAAG GTTACATGAA AGTCAGCATG
    TTCATCCTGG GAACTGGAGA TGAGCCTCCT GCTGAGAAGC GAGATCGTGA TAATGACAGT
    GATGACGTGG AGAGTAATTT GTTACTCCCT GCTGGCATCG CCCTCCGGTG GGTGACCTTC
    TTGCTTAAAA TCTACCGAGC AGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA
    GTAAAGGAAA TATTTGGAGG CAATGCAGAT AAAAAAAACC TAGTGGATCC TTTTGTAGAA
    GTTTCCTTTG CTGGAAAAAA GGTTTGCACA AATATAATTG AGAAAAATGC AAATCCTGAG
    TGGAACCAAG TCGTTAATCT TCAGATAAAG TTTCCTTCAA TGTGTGAAAA AATAAAACTA
    ACAATATATG ACTGGGACCG TCTTACTAAA AATGATGTTG TTGGAACAAC ATATCTACAC
    CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGATC TCTCATCTTC GGGATCTGGG
    GCTGCGTCAT ATACAGCAAA TACAGGAGAA ACAGAGGTGG GCTTTGTTCC AACATTTGGG
    CCTTGTTATC TGAACCTTTA CGGAAGCCCT AGAGAGTACA CAGGATTCCC AGACCCCTAT
    GATGAGCTAA ATACTGGAAA GGGGGAAGGA GTTGCCTACA GAGGCAGAAT CTTGGTTGAA
    TTAAGCACTT TTCTTGAAAA GACACCACCA GACAAAAAGC TTGAGCCTAT TTCAAATGAT
    GACCTGCTGG TTGTGGAGAA ATACCAACGA AGGCGGAAGT ATAGCCTGTC TGCCGTGTTC
    CATTCGGCCA CCATGTTGCA AGATGTTGGC GAGGCCATTC AGTTTGAAGT CAGCATTGGG
    AACTATGGCA ACAAGTTTGA CACGACTTGT AAGCCTTTGG CATCAACCAC TCAATACAGC
    CGTGCCGTAT TTGATGGGAA CTACTATTAT TACTTGCCTT GGGCCCACAC AAAGCCGGTT
    GTTACCCTGA CTTCATACTG GGAGGACATC AGTCATCGCC TGGATGCAGT GAACACTCTC
    CTAACCATGG CAGAACGGCT GCAATTGAAC TTAGAGGCTC TGAAATCAGG GATGCAAGGT
    AAAGTTCCTG CAAACCAGTT GGCTGAAATA TGGTTGAAGC TGATCGATGA AGTTATAGAA
    GATACAAGGT ACACATTGCC TGTCACGGAA GGAAAAGCTA ATGTCACAAT TCTTGACACT
    CAGATCCGAA AGTTGCGGTC CCGATCTCTC TCCCAGATTC ATGAGGCAGC TGTGAGGATG
    AGGTCTGAGG CCACAGAGGT GAAATCCACA CTGGCAGAAA TTGAGGACTG GCTTGAAAAA
    TTACTGCAAC TGACTGAAGA GCCACAGAAC AGTATGCCTG ACATTATCAT CTGGATGATC
    CGGGGGGAGA AAAGACTGGC CTATGCACGC ATCCCTGCCC ATCAGGTTCT ATACTCCACC
    AGTGGTGAGA CTGCATCTGG GAAATACTGC GGGAAAACCC AAACCATCTT GCTGAAGTAT
    CCGCAGGAAA AAACCCACGG ACCAAAGGTG CCCGCAGAGC TGCGAGTCAA CATCTGGCTG
    GGCTTAAGTG CTGTTGAGAA GAAATTCAAT AGCTTCGCAG AAGGAACATT CACCGTCTTT
    GCTGAAATGT ATGAAAATCA AGCTCTTATG TTTGGAAAAT GGGGCACTTC TGGATTAGTG
    GGTCGTCATA AATTTTCTGA TGTCACAGGA AAAATAAAAC TCAAGAGGGA ATTCTTTTTG
    CCCCCGAAAG GCTGGGAATG GGAAGGAGAG TGGATAGTTG ATCCTGAAAG AAGCTTGCTG
    ACTGAAGCAG ACGCAGGGCA CACGGAGTTC ACGGACGAAG TGTACCAGAA TGAGAGCCGC
    TACCCTGGGG GTGACTGGAA GCCAGCTGAG GACACCTATA CCGATGCGAA TGGTGATAAA
    GCGGCATCAC CTAGCGAGCT GACATGCCCT CCAGGATGGG AATGGGAAGA TGACGCATGG
    GTATATGATA TAAATCGTGC AGTGGATGAG AAAGGCTGGG AGTATGGAAT TACCATTCCT
    CCTGATACTA AACCCAAATC CTGGGTTGCA GCAGAGAAAA TGTATCATAC TCATAGACGA
    CGAAGGCTAG TCCGAAAACG CAAGAAAGAT TTAACACAAA GCACTGCAAG CACTGCAAGG
    GCCATGGAAG AATTGGAAGA CCGGGAGGGC TGGGAATATG CTTCTTTAAT TGGCTGGAAA
    TTTCACTGGA AACAGCGTAG CTCAGATACC TTCCGCCGTA GACGCTGGAG AAGGAAAATG
    GCTCCTTCGG AAACGCACGG TGCGGCCGCC ATCTTTAAAC TGGAGGGCGC CCTCGGTGCA
    GACATGACCG AGGATGGAGA CGAGAAGAGT GTGGACAAGC AGAAGTATAG TGCCACCACT
    GTATTTGGGG CAAACACTCC CATTGTTTCC TGTAACTTTG ACAGAGTCTA CATTTACCAT
    CTGCGCTGCT ATATCTATCA AGCCAGAAAC CTCATGGCTC TAGACAAGGA TAGTTTTTCA
    GATCCATATG CACATGTCTC CTTCCTCCAT CGAAGCAAAA CCACTGAGAT CATCCACTCG
    ACTCTGAATC CCACATGGGA CCAGACAATT ATATTTGATG AAATCGAAAT CTATGGGGAA
    CCCCAGACAG TTCTACAGAA CCCACCCAAA GTTATCATCG AACTTTTTGA CAATGACCAA
    GTGGGCAAAG ATGAATTTTT AGGACGAAGC ATGTGCTCTC CTCTGGTAAA ACTAAACTCA
    GAAACGGATG TCACACCCAA ACTTCTCTGG CACCCTGTAA TGAATGGAGA CAAAGCCTGT
    GGGGATGTCC TTGTAACTGC AGAGCTGATT CTAAGGCACA AGGATGGTTC CAATCTTCCC
    ATCCTTCCCT CACAAAGGGC ACCAAATCTA TACATGGTCC CACAAGGGAT CAGGCCTGTG
    GTCCAACTCA CTGCTATTGA GATTCTAGCT TGGGGCTTAA GAAATATGAA AAACTACCAG
    ATGGCTTCTA TCACCTCCCC CAGCCTCATT GTGGAGTGTG GAGGGGAAAG GGTAGAATCA
    GTGGTGATCA AAAACCTGAA GAAGACACCC AACTTCCCAA GTTCTGTTCT CTTCATGAAA
    GTGTTCTTGC CCAAGGAGGA ACTGTACATG CCTCCGCTGG TGATAAAGGT CATCGACCAC
    AGGCAGTTCG GGCGGAAGCC CGTTGTTGGG CAGTGCACCA TCGATCGTCT GGACCGCTTT
    CGTTGTGACC CTTATGCGGG AAAGGAGGAT CTTGTGCCCC AGCTAAAAGC CTCCCTTCTA
    TCTACCCCAC CCTGCCGGGA TGTCATTATC GAAATAGAAG ACACCAAACC ATTACTGGCT
    TCGAAGCTGA CAGAAAAGGA GGAAGAAATC GTTGACTGGT GGAGTAAATT TTATGCTTCC
    ACAGGAGAAC ATGAAAAATG TGGACAATAT GTTCAGAAAG GATATTCGAA ACTCAAGATA
    TATGATTGTG AACTAGAGGA TGTAACAGAA TTTGAAGGCC TGACAGACTT CTCAGATACT
    TTTAAGCTGT ATCGAGGCAA ATCAGATGAA AATGAAGATC CCTCAGTGGT AGGAGAGTTT
    AAGGGCTCCT TTAGAATCTA CTCTCTGCCG GATGACCCCA GCGTGCCGGC CCCTCCCAGG
    CAGTTTCGGG AATTACCTGA CAGCGTCCCA CAGGAATGCA CGGTTAGGAT TTACATTGTT
    CGAGGCTTAG AGCTCCAGCC CCAGGACAAC AATGGCCTGT GTGACCCTTA TATAAAAATA
    ACACTGGGCA AAAAAGTAAT AGAAGATCGA GACCACTATA TCCCCAACAC CCTCAACCCA
    GTTTTCGGCA GAATGTATGA ACTGAGCTGC TACTTACCTC AAGAAAAAGA TCTGAAAATT
    TCTGTCTATG ACTACGACAC CTTTACCCGT GATGAAAAGG TGGGAGAAAC AATTATTGAT
    CTGGAAAACC GATTCCTTTC CCGGTTTGGG TCTCACTGCG GCATACCTGA GCAGTACTGT
    GTTTCTGGAG TTAACACCTG GCGAGATCAA CTGAGACCAA CTCAGTTGCT TCAAAATGTA
    GCCAGGTTCA AAGGCTTCCC ACCACCCATC CTTTCTGAAG ATGGAAATAG AATCAGATAT
    GGAGGGCGAG ACTACAGTTT GGATGAATTT GGGGAAACTT CAGATGTGGG TGGATGTTTT
    CCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe283360
    Clone ID Related Accession (Same CDS sequence) XM_022412117.1
    Accession Version XM_022412117.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6174bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product myoferlin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006610.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGCTGCGGG TGATCGTGGA GTCCGCCAGC AATATCCCGA AAACGAAATT TGGGAAACCG 
    GATCCTATCG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGACAAC
    GAACTCAATC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGAGGGGTAT ACCACTGGAC
    TTTTCATCTT CCCTTACAAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAATTA
    ATTGGCACGG CCACTGTAGC CCTGAAGGAC CTGACTGGTG ACCAGAGCAG ATCACTGCCG
    TACAAACTCA TCTCCCTGCT CAGCGAAAAG GGGCAAGATA CTGGGGCCAC CATTGACTTG
    GTGATTGGCT ATGATCCACC TTCTGCTCCA CATCCAAATG ACCCCAGTGG GACCAGTGTG
    TCAGTCACAG AAGGAGAAGA AGAGGAAGAT GGAGGTGATG AAGACAGGTT GGATGGCACA
    GTCACGGGCC CTGGGCCCAG GGGGTCAGGT GGGATCGTGT CGGAGGCTCA CCTTGCCCGG
    AGGCTCACGA AAGCAAAGAA GAACCGGCGG ATGCTGTCCA ACAAGCCTCA GGACTTCCAG
    ATCCGCGTCC GAGTGATTGA GGGCCGTCAG TTAAGTGGCA ACAACATCAA GCCTGTGGTC
    AAGGTCCATG TCTGTGGCCA GACACACAGA ACAAGGATCA AGAGAGGGAA CAACCCATTT
    TATGATGAGT TATTTTTCTA CAATGTCCAC ATAACCCCTT CTGAACTGAT GGATGAGATC
    ATCAGCATTC GGGTTTATAA TTCCCATTCT TTGCGGGCAG ATTGTCTGAT GGGGGAATTT
    AAGATTGATG TTGGATTCAT TTATGATGAA CCGGGTCACG CTGTCATGAG AAAATGGCTT
    CTTCTCAATG ACCCTGAAGA TGCCAGCTCA GGTGCTAAAG GTTACATGAA AGTCAGCATG
    TTCATCCTGG GAACTGGAGA TGAGCCTCCT GCTGAGAAGC GAGATCGTGA TAATGACAGT
    GATGACGTGG AGAGTAATTT GTTACTCCCT GCTGGCATCG CCCTCCGGTG GGTGACCTTC
    TTGCTTAAAA TCTACCGAGC AGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA
    GTAAAGGAAA TATTTGGAGG CAATGCAGAT AAAAAAAACC TAGTGGATCC TTTTGTAGAA
    GTTTCCTTTG CTGGAAAAAA GGTTTGCACA AATATAATTG AGAAAAATGC AAATCCTGAG
    TGGAACCAAG TCGTTAATCT TCAGATAAAG TTTCCTTCAA TGTGTGAAAA AATAAAACTA
    ACAATATATG ACTGGGACCG TCTTACTAAA AATGATGTTG TTGGAACAAC ATATCTACAC
    CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGATC TCTCATCTTC GGGATCTGGG
    GCTGCGTCAT ATACAGCAAA TACAGGAGAA ACAGAGGTGG GCTTTGTTCC AACATTTGGG
    CCTTGTTATC TGAACCTTTA CGGAAGCCCT AGAGAGTACA CAGGATTCCC AGACCCCTAT
    GATGAGCTAA ATACTGGAAA GGGGGAAGGA GTTGCCTACA GAGGCAGAAT CTTGGTTGAA
    TTAAGCACTT TTCTTGAAAA GACACCACCA GACAAAAAGC TTGAGCCTAT TTCAAATGAT
    GACCTGCTGG TTGTGGAGAA ATACCAACGA AGGCGGAAGT ATAGCCTGTC TGCCGTGTTC
    CATTCGGCCA CCATGTTGCA AGATGTTGGC GAGGCCATTC AGTTTGAAGT CAGCATTGGG
    AACTATGGCA ACAAGTTTGA CACGACTTGT AAGCCTTTGG CATCAACCAC TCAATACAGC
    CGTGCCGTAT TTGATGGGAA CTACTATTAT TACTTGCCTT GGGCCCACAC AAAGCCGGTT
    GTTACCCTGA CTTCATACTG GGAGGACATC AGTCATCGCC TGGATGCAGT GAACACTCTC
    CTAACCATGG CAGAACGGCT GCAATTGAAC TTAGAGGCTC TGAAATCAGG GATGCAAGGT
    AAAGTTCCTG CAAACCAGTT GGCTGAAATA TGGTTGAAGC TGATCGATGA AGTTATAGAA
    GATACAAGGT ACACATTGCC TGTCACGGAA GGAAAAGCTA ATGTCACAAT TCTTGACACT
    CAGATCCGAA AGTTGCGGTC CCGATCTCTC TCCCAGATTC ATGAGGCAGC TGTGAGGATG
    AGGTCTGAGG CCACAGAGGT GAAATCCACA CTGGCAGAAA TTGAGGACTG GCTTGAAAAA
    TTACTGCAAC TGACTGAAGA GCCACAGAAC AGTATGCCTG ACATTATCAT CTGGATGATC
    CGGGGGGAGA AAAGACTGGC CTATGCACGC ATCCCTGCCC ATCAGGTTCT ATACTCCACC
    AGTGGTGAGA CTGCATCTGG GAAATACTGC GGGAAAACCC AAACCATCTT GCTGAAGTAT
    CCGCAGGAAA AAACCCACGG ACCAAAGGTG CCCGCAGAGC TGCGAGTCAA CATCTGGCTG
    GGCTTAAGTG CTGTTGAGAA GAAATTCAAT AGCTTCGCAG AAGGAACATT CACCGTCTTT
    GCTGAAATGT ATGAAAATCA AGCTCTTATG TTTGGAAAAT GGGGCACTTC TGGATTAGTG
    GGTCGTCATA AATTTTCTGA TGTCACAGGA AAAATAAAAC TCAAGAGGGA ATTCTTTTTG
    CCCCCGAAAG GCTGGGAATG GGAAGGAGAG TGGATAGTTG ATCCTGAAAG AAGCTTGCTG
    ACTGAAGCAG ACGCAGGGCA CACGGAGTTC ACGGACGAAG TGTACCAGAA TGAGAGCCGC
    TACCCTGGGG GTGACTGGAA GCCAGCTGAG GACACCTATA CCGATGCGAA TGGTGATAAA
    GCGGCATCAC CTAGCGAGCT GACATGCCCT CCAGGATGGG AATGGGAAGA TGACGCATGG
    GTATATGATA TAAATCGTGC AGTGGATGAG AAAGGCTGGG AGTATGGAAT TACCATTCCT
    CCTGATACTA AACCCAAATC CTGGGTTGCA GCAGAGAAAA TGTATCATAC TCATAGACGA
    CGAAGGCTAG TCCGAAAACG CAAGAAAGAT TTAACACAAA GCACTGCAAG CACTGCAAGG
    GCCATGGAAG AATTGGAAGA CCGGGAGGGC TGGGAATATG CTTCTTTAAT TGGCTGGAAA
    TTTCACTGGA AACAGCGTAG CTCAGATACC TTCCGCCGTA GACGCTGGAG AAGGAAAATG
    GCTCCTTCGG AAACGCACGG TGCGGCCGCC ATCTTTAAAC TGGAGGGCGC CCTCGGTGCA
    GACATGACCG AGGATGGAGA CGAGAAGAGT GTGGACAAGC AGAAGTATAG TGCCACCACT
    GTATTTGGGG CAAACACTCC CATTGTTTCC TGTAACTTTG ACAGAGTCTA CATTTACCAT
    CTGCGCTGCT ATATCTATCA AGCCAGAAAC CTCATGGCTC TAGACAAGGA TAGTTTTTCA
    GATCCATATG CACATGTCTC CTTCCTCCAT CGAAGCAAAA CCACTGAGAT CATCCACTCG
    ACTCTGAATC CCACATGGGA CCAGACAATT ATATTTGATG AAATCGAAAT CTATGGGGAA
    CCCCAGACAG TTCTACAGAA CCCACCCAAA GTTATCATCG AACTTTTTGA CAATGACCAA
    GTGGGCAAAG ATGAATTTTT AGGACGAAGC ATGTGCTCTC CTCTGGTAAA ACTAAACTCA
    GAAACGGATG TCACACCCAA ACTTCTCTGG CACCCTGTAA TGAATGGAGA CAAAGCCTGT
    GGGGATGTCC TTGTAACTGC AGAGCTGATT CTAAGGCACA AGGATGGTTC CAATCTTCCC
    ATCCTTCCCT CACAAAGGGC ACCAAATCTA TACATGGTCC CACAAGGGAT CAGGCCTGTG
    GTCCAACTCA CTGCTATTGA GATTCTAGCT TGGGGCTTAA GAAATATGAA AAACTACCAG
    ATGGCTTCTA TCACCTCCCC CAGCCTCATT GTGGAGTGTG GAGGGGAAAG GGTAGAATCA
    GTGGTGATCA AAAACCTGAA GAAGACACCC AACTTCCCAA GTTCTGTTCT CTTCATGAAA
    GTGTTCTTGC CCAAGGAGGA ACTGTACATG CCTCCGCTGG TGATAAAGGT CATCGACCAC
    AGGCAGTTCG GGCGGAAGCC CGTTGTTGGG CAGTGCACCA TCGATCGTCT GGACCGCTTT
    CGTTGTGACC CTTATGCGGG AAAGGAGGAT CTTGTGCCCC AGCTAAAAGC CTCCCTTCTA
    TCTACCCCAC CCTGCCGGGA TGTCATTATC GAAATAGAAG ACACCAAACC ATTACTGGCT
    TCGAAGGAGG AAGAAATCGT TGACTGGTGG AGTAAATTTT ATGCTTCCAC AGGAGAACAT
    GAAAAATGTG GACAATATGT TCAGAAAGGA TATTCGAAAC TCAAGATATA TGATTGTGAA
    CTAGAGGATG TAACAGAATT TGAAGGCCTG ACAGACTTCT CAGATACTTT TAAGCTGTAT
    CGAGGCAAAT CAGATGAAAA TGAAGATCCC TCAGTGGTAG GAGAGTTTAA GGGCTCCTTT
    AGAATCTACT CTCTGCCGGA TGACCCCAGC GTGCCGGCCC CTCCCAGGCA GTTTCGGGAA
    TTACCTGACA GCGTCCCACA GGAATGCACG GTTAGGATTT ACATTGTTCG AGGCTTAGAG
    CTCCAGCCCC AGGACAACAA TGGCCTGTGT GACCCTTATA TAAAAATAAC ACTGGGCAAA
    AAAGTAATAG AAGATCGAGA CCACTATATC CCCAACACCC TCAACCCAGT TTTCGGCAGA
    ATGTATGAAC TGAGCTGCTA CTTACCTCAA GAAAAAGATC TGAAAATTTC TGTCTATGAC
    TACGACACCT TTACCCGTGA TGAAAAGGTG GGAGAAACAA TTATTGATCT GGAAAACCGA
    TTCCTTTCCC GGTTTGGGTC TCACTGCGGC ATACCTGAGC AGTACTGTGT TTCTGGAGTT
    AACACCTGGC GAGATCAACT GAGACCAACT CAGTTGCTTC AAAATGTAGC CAGGTTCAAA
    GGCTTCCCAC CACCCATCCT TTCTGAAGAT GGAAATAGAA TCAGATATGG AGGGCGAGAC
    TACAGTTTGG ATGAATTTGA AGCCAACAAA ATCCTGCACC AGCACCTCGG GGCCCCCGAT
    GAGCGTCTGG CCCTTCACAT CCTCAGGACT CAGGGGCTGG TCCCCGAGCA TGTGGAAACG
    AGGACTTTGC ACAGCACCTT CCAGCCCAAC ATTTCCCAGG GGAAACTTCA GATGTGGGTG
    GATGTTTTCC CTAAGAGTTT GGGGCCACCA GGCCCTCCTT TCAACATCAC CCCCAGGAAA
    GCTAAAAAAT ACTACCTGCG TGTGATCATC TGGAATACCA AGGACGTTCT CTTGGATGAG
    AAGAGCATCA CAGGAGAGGA AATGAGTGAC ATCTATGTCA AAGGCTGGAT TCCTGGCAAT
    GAAGAAAACA AACAGAAAAC AGATGTCCAT TACAGGTCCT TGGACGGGGA AGGGAATTTT
    AACTGGAGAT TTGTTTTCCC ATTTGACTAC CTCCCAGCTG AACAACTCTG TATCGTTGCT
    AAAAAAGAGC ATTTCTGGAG TATTGACCAA ACTGAATTTC GGGTCCCACC CAGACTGATC
    ATTCAGATAT GGGATAATGA CAAGTTTTCT CTGGATGACT ATTTGGGTTT CCTGGAACTT
    GATTTGCATC ACACCATCAT TCCAGCAAAA TCATCAGAGA AATGCAGTTT GGATATGATT
    CCAGACCTCA AAGCCATGAA CCCCCTTAAA GCAAAAACTG CCTCACTGTT TGAGCAGAAG
    TCCATGAAAG GCTGGTGGCC GTGCTACGCA GATAAAGATG GCTCCCGCGT GATGGCTGGG
    AAAGTGGAGA TGACGTTGGA AATCCTCAAT GAGAAGGAGG CAGACGAGAG GCCAGCCGGG
    AAGGGACGGG ATGAACCCAA CATGAACCCC AAGCTGGACC CACCAAACCG ACCAGAAACC
    TCCTTCCTCT GGTTCACCAA CCCATGTAAG ACCATGAAGT TCATTGTGTG GCGCCGATTT
    AAGTGGGTGA TCATTGGCCT GCTGATCCTA CTGATCCTGC TGCTCTTTGT GGCCGTGCTC
    CTGTACTCCT TACCGAATTA TTTGTCAATG AAGATTGTGA GGCCAAATGC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022267825.1
    CDS120..6293
    Translation

    Target ORF information:

    RefSeq Version XM_022412117.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris myoferlin (MYOF), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022412117.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGCTGCGGG TGATCGTGGA GTCCGCCAGC AATATCCCGA AAACGAAATT TGGGAAACCG 
    GATCCTATCG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGACAAC
    GAACTCAATC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGAGGGGTAT ACCACTGGAC
    TTTTCATCTT CCCTTACAAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAATTA
    ATTGGCACGG CCACTGTAGC CCTGAAGGAC CTGACTGGTG ACCAGAGCAG ATCACTGCCG
    TACAAACTCA TCTCCCTGCT CAGCGAAAAG GGGCAAGATA CTGGGGCCAC CATTGACTTG
    GTGATTGGCT ATGATCCACC TTCTGCTCCA CATCCAAATG ACCCCAGTGG GACCAGTGTG
    TCAGTCACAG AAGGAGAAGA AGAGGAAGAT GGAGGTGATG AAGACAGGTT GGATGGCACA
    GTCACGGGCC CTGGGCCCAG GGGGTCAGGT GGGATCGTGT CGGAGGCTCA CCTTGCCCGG
    AGGCTCACGA AAGCAAAGAA GAACCGGCGG ATGCTGTCCA ACAAGCCTCA GGACTTCCAG
    ATCCGCGTCC GAGTGATTGA GGGCCGTCAG TTAAGTGGCA ACAACATCAA GCCTGTGGTC
    AAGGTCCATG TCTGTGGCCA GACACACAGA ACAAGGATCA AGAGAGGGAA CAACCCATTT
    TATGATGAGT TATTTTTCTA CAATGTCCAC ATAACCCCTT CTGAACTGAT GGATGAGATC
    ATCAGCATTC GGGTTTATAA TTCCCATTCT TTGCGGGCAG ATTGTCTGAT GGGGGAATTT
    AAGATTGATG TTGGATTCAT TTATGATGAA CCGGGTCACG CTGTCATGAG AAAATGGCTT
    CTTCTCAATG ACCCTGAAGA TGCCAGCTCA GGTGCTAAAG GTTACATGAA AGTCAGCATG
    TTCATCCTGG GAACTGGAGA TGAGCCTCCT GCTGAGAAGC GAGATCGTGA TAATGACAGT
    GATGACGTGG AGAGTAATTT GTTACTCCCT GCTGGCATCG CCCTCCGGTG GGTGACCTTC
    TTGCTTAAAA TCTACCGAGC AGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA
    GTAAAGGAAA TATTTGGAGG CAATGCAGAT AAAAAAAACC TAGTGGATCC TTTTGTAGAA
    GTTTCCTTTG CTGGAAAAAA GGTTTGCACA AATATAATTG AGAAAAATGC AAATCCTGAG
    TGGAACCAAG TCGTTAATCT TCAGATAAAG TTTCCTTCAA TGTGTGAAAA AATAAAACTA
    ACAATATATG ACTGGGACCG TCTTACTAAA AATGATGTTG TTGGAACAAC ATATCTACAC
    CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGATC TCTCATCTTC GGGATCTGGG
    GCTGCGTCAT ATACAGCAAA TACAGGAGAA ACAGAGGTGG GCTTTGTTCC AACATTTGGG
    CCTTGTTATC TGAACCTTTA CGGAAGCCCT AGAGAGTACA CAGGATTCCC AGACCCCTAT
    GATGAGCTAA ATACTGGAAA GGGGGAAGGA GTTGCCTACA GAGGCAGAAT CTTGGTTGAA
    TTAAGCACTT TTCTTGAAAA GACACCACCA GACAAAAAGC TTGAGCCTAT TTCAAATGAT
    GACCTGCTGG TTGTGGAGAA ATACCAACGA AGGCGGAAGT ATAGCCTGTC TGCCGTGTTC
    CATTCGGCCA CCATGTTGCA AGATGTTGGC GAGGCCATTC AGTTTGAAGT CAGCATTGGG
    AACTATGGCA ACAAGTTTGA CACGACTTGT AAGCCTTTGG CATCAACCAC TCAATACAGC
    CGTGCCGTAT TTGATGGGAA CTACTATTAT TACTTGCCTT GGGCCCACAC AAAGCCGGTT
    GTTACCCTGA CTTCATACTG GGAGGACATC AGTCATCGCC TGGATGCAGT GAACACTCTC
    CTAACCATGG CAGAACGGCT GCAATTGAAC TTAGAGGCTC TGAAATCAGG GATGCAAGGT
    AAAGTTCCTG CAAACCAGTT GGCTGAAATA TGGTTGAAGC TGATCGATGA AGTTATAGAA
    GATACAAGGT ACACATTGCC TGTCACGGAA GGAAAAGCTA ATGTCACAAT TCTTGACACT
    CAGATCCGAA AGTTGCGGTC CCGATCTCTC TCCCAGATTC ATGAGGCAGC TGTGAGGATG
    AGGTCTGAGG CCACAGAGGT GAAATCCACA CTGGCAGAAA TTGAGGACTG GCTTGAAAAA
    TTACTGCAAC TGACTGAAGA GCCACAGAAC AGTATGCCTG ACATTATCAT CTGGATGATC
    CGGGGGGAGA AAAGACTGGC CTATGCACGC ATCCCTGCCC ATCAGGTTCT ATACTCCACC
    AGTGGTGAGA CTGCATCTGG GAAATACTGC GGGAAAACCC AAACCATCTT GCTGAAGTAT
    CCGCAGGAAA AAACCCACGG ACCAAAGGTG CCCGCAGAGC TGCGAGTCAA CATCTGGCTG
    GGCTTAAGTG CTGTTGAGAA GAAATTCAAT AGCTTCGCAG AAGGAACATT CACCGTCTTT
    GCTGAAATGT ATGAAAATCA AGCTCTTATG TTTGGAAAAT GGGGCACTTC TGGATTAGTG
    GGTCGTCATA AATTTTCTGA TGTCACAGGA AAAATAAAAC TCAAGAGGGA ATTCTTTTTG
    CCCCCGAAAG GCTGGGAATG GGAAGGAGAG TGGATAGTTG ATCCTGAAAG AAGCTTGCTG
    ACTGAAGCAG ACGCAGGGCA CACGGAGTTC ACGGACGAAG TGTACCAGAA TGAGAGCCGC
    TACCCTGGGG GTGACTGGAA GCCAGCTGAG GACACCTATA CCGATGCGAA TGGTGATAAA
    GCGGCATCAC CTAGCGAGCT GACATGCCCT CCAGGATGGG AATGGGAAGA TGACGCATGG
    GTATATGATA TAAATCGTGC AGTGGATGAG AAAGGCTGGG AGTATGGAAT TACCATTCCT
    CCTGATACTA AACCCAAATC CTGGGTTGCA GCAGAGAAAA TGTATCATAC TCATAGACGA
    CGAAGGCTAG TCCGAAAACG CAAGAAAGAT TTAACACAAA GCACTGCAAG CACTGCAAGG
    GCCATGGAAG AATTGGAAGA CCGGGAGGGC TGGGAATATG CTTCTTTAAT TGGCTGGAAA
    TTTCACTGGA AACAGCGTAG CTCAGATACC TTCCGCCGTA GACGCTGGAG AAGGAAAATG
    GCTCCTTCGG AAACGCACGG TGCGGCCGCC ATCTTTAAAC TGGAGGGCGC CCTCGGTGCA
    GACATGACCG AGGATGGAGA CGAGAAGAGT GTGGACAAGC AGAAGTATAG TGCCACCACT
    GTATTTGGGG CAAACACTCC CATTGTTTCC TGTAACTTTG ACAGAGTCTA CATTTACCAT
    CTGCGCTGCT ATATCTATCA AGCCAGAAAC CTCATGGCTC TAGACAAGGA TAGTTTTTCA
    GATCCATATG CACATGTCTC CTTCCTCCAT CGAAGCAAAA CCACTGAGAT CATCCACTCG
    ACTCTGAATC CCACATGGGA CCAGACAATT ATATTTGATG AAATCGAAAT CTATGGGGAA
    CCCCAGACAG TTCTACAGAA CCCACCCAAA GTTATCATCG AACTTTTTGA CAATGACCAA
    GTGGGCAAAG ATGAATTTTT AGGACGAAGC ATGTGCTCTC CTCTGGTAAA ACTAAACTCA
    GAAACGGATG TCACACCCAA ACTTCTCTGG CACCCTGTAA TGAATGGAGA CAAAGCCTGT
    GGGGATGTCC TTGTAACTGC AGAGCTGATT CTAAGGCACA AGGATGGTTC CAATCTTCCC
    ATCCTTCCCT CACAAAGGGC ACCAAATCTA TACATGGTCC CACAAGGGAT CAGGCCTGTG
    GTCCAACTCA CTGCTATTGA GATTCTAGCT TGGGGCTTAA GAAATATGAA AAACTACCAG
    ATGGCTTCTA TCACCTCCCC CAGCCTCATT GTGGAGTGTG GAGGGGAAAG GGTAGAATCA
    GTGGTGATCA AAAACCTGAA GAAGACACCC AACTTCCCAA GTTCTGTTCT CTTCATGAAA
    GTGTTCTTGC CCAAGGAGGA ACTGTACATG CCTCCGCTGG TGATAAAGGT CATCGACCAC
    AGGCAGTTCG GGCGGAAGCC CGTTGTTGGG CAGTGCACCA TCGATCGTCT GGACCGCTTT
    CGTTGTGACC CTTATGCGGG AAAGGAGGAT CTTGTGCCCC AGCTAAAAGC CTCCCTTCTA
    TCTACCCCAC CCTGCCGGGA TGTCATTATC GAAATAGAAG ACACCAAACC ATTACTGGCT
    TCGAAGGAGG AAGAAATCGT TGACTGGTGG AGTAAATTTT ATGCTTCCAC AGGAGAACAT
    GAAAAATGTG GACAATATGT TCAGAAAGGA TATTCGAAAC TCAAGATATA TGATTGTGAA
    CTAGAGGATG TAACAGAATT TGAAGGCCTG ACAGACTTCT CAGATACTTT TAAGCTGTAT
    CGAGGCAAAT CAGATGAAAA TGAAGATCCC TCAGTGGTAG GAGAGTTTAA GGGCTCCTTT
    AGAATCTACT CTCTGCCGGA TGACCCCAGC GTGCCGGCCC CTCCCAGGCA GTTTCGGGAA
    TTACCTGACA GCGTCCCACA GGAATGCACG GTTAGGATTT ACATTGTTCG AGGCTTAGAG
    CTCCAGCCCC AGGACAACAA TGGCCTGTGT GACCCTTATA TAAAAATAAC ACTGGGCAAA
    AAAGTAATAG AAGATCGAGA CCACTATATC CCCAACACCC TCAACCCAGT TTTCGGCAGA
    ATGTATGAAC TGAGCTGCTA CTTACCTCAA GAAAAAGATC TGAAAATTTC TGTCTATGAC
    TACGACACCT TTACCCGTGA TGAAAAGGTG GGAGAAACAA TTATTGATCT GGAAAACCGA
    TTCCTTTCCC GGTTTGGGTC TCACTGCGGC ATACCTGAGC AGTACTGTGT TTCTGGAGTT
    AACACCTGGC GAGATCAACT GAGACCAACT CAGTTGCTTC AAAATGTAGC CAGGTTCAAA
    GGCTTCCCAC CACCCATCCT TTCTGAAGAT GGAAATAGAA TCAGATATGG AGGGCGAGAC
    TACAGTTTGG ATGAATTTGA AGCCAACAAA ATCCTGCACC AGCACCTCGG GGCCCCCGAT
    GAGCGTCTGG CCCTTCACAT CCTCAGGACT CAGGGGCTGG TCCCCGAGCA TGTGGAAACG
    AGGACTTTGC ACAGCACCTT CCAGCCCAAC ATTTCCCAGG GGAAACTTCA GATGTGGGTG
    GATGTTTTCC CTAAGAGTTT GGGGCCACCA GGCCCTCCTT TCAACATCAC CCCCAGGAAA
    GCTAAAAAAT ACTACCTGCG TGTGATCATC TGGAATACCA AGGACGTTCT CTTGGATGAG
    AAGAGCATCA CAGGAGAGGA AATGAGTGAC ATCTATGTCA AAGGCTGGAT TCCTGGCAAT
    GAAGAAAACA AACAGAAAAC AGATGTCCAT TACAGGTCCT TGGACGGGGA AGGGAATTTT
    AACTGGAGAT TTGTTTTCCC ATTTGACTAC CTCCCAGCTG AACAACTCTG TATCGTTGCT
    AAAAAAGAGC ATTTCTGGAG TATTGACCAA ACTGAATTTC GGGTCCCACC CAGACTGATC
    ATTCAGATAT GGGATAATGA CAAGTTTTCT CTGGATGACT ATTTGGGTTT CCTGGAACTT
    GATTTGCATC ACACCATCAT TCCAGCAAAA TCATCAGAGA AATGCAGTTT GGATATGATT
    CCAGACCTCA AAGCCATGAA CCCCCTTAAA GCAAAAACTG CCTCACTGTT TGAGCAGAAG
    TCCATGAAAG GCTGGTGGCC GTGCTACGCA GATAAAGATG GCTCCCGCGT GATGGCTGGG
    AAAGTGGAGA TGACGTTGGA AATCCTCAAT GAGAAGGAGG CAGACGAGAG GCCAGCCGGG
    AAGGGACGGG ATGAACCCAA CATGAACCCC AAGCTGGACC CACCAAACCG ACCAGAAACC
    TCCTTCCTCT GGTTCACCAA CCCATGTAAG ACCATGAAGT TCATTGTGTG GCGCCGATTT
    AAGTGGGTGA TCATTGGCCT GCTGATCCTA CTGATCCTGC TGCTCTTTGT GGCCGTGCTC
    CTGTACTCCT TACCGAATTA TTTGTCAATG AAGATTGTGA GGCCAAATGC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27786
    Clone ID Related Accession (Same CDS sequence) XM_853765.4 , XM_853765.5
    Accession Version XM_853765.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6186bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product myoferlin isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_853765.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTGCGGG TGATCGTGGA GTCCGCCAGC AATATCCCGA AAACGAAATT TGGGAAACCG 
    GATCCTATCG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGACAAC
    GAACTCAATC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGAGGGGTAT ACCACTGGAC
    TTTTCATCTT CCCTTACAAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAATTA
    ATTGGCACGG CCACTGTAGC CCTGAAGGAC CTGACTGGTG ACCAGAGCAG ATCACTGCCG
    TACAAACTCA TCTCCCTGCT CAGCGAAAAG GGGCAAGATA CTGGGGCCAC CATTGACTTG
    GTGATTGGCT ATGATCCACC TTCTGCTCCA CATCCAAATG ACCCCAGTGG GACCAGTGTG
    TCAGTCACAG AAGGAGAAGA AGAGGAAGAT GGAGGTGATG AAGACAGGTT GGATGGCACA
    GTCACGGGCC CTGGGCCCAG GGGGTCAGGT GGGATCGTGT CGGAGGCTCA CCTTGCCCGG
    AGGCTCACGA AAGCAAAGAA GAACCGGCGG ATGCTGTCCA ACAAGCCTCA GGACTTCCAG
    ATCCGCGTCC GAGTGATTGA GGGCCGTCAG TTAAGTGGCA ACAACATCAA GCCTGTGGTC
    AAGGTCCATG TCTGTGGCCA GACACACAGA ACAAGGATCA AGAGAGGGAA CAACCCATTT
    TATGATGAGT TATTTTTCTA CAATGTCCAC ATAACCCCTT CTGAACTGAT GGATGAGATC
    ATCAGCATTC GGGTTTATAA TTCCCATTCT TTGCGGGCAG ATTGTCTGAT GGGGGAATTT
    AAGATTGATG TTGGATTCAT TTATGATGAA CCGGGTCACG CTGTCATGAG AAAATGGCTT
    CTTCTCAATG ACCCTGAAGA TGCCAGCTCA GGTGCTAAAG GTTACATGAA AGTCAGCATG
    TTCATCCTGG GAACTGGAGA TGAGCCTCCT GCTGAGAAGC GAGATCGTGA TAATGACAGT
    GATGACGTGG AGAGTAATTT GTTACTCCCT GCTGGCATCG CCCTCCGGTG GGTGACCTTC
    TTGCTTAAAA TCTACCGAGC AGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA
    GTAAAGGAAA TATTTGGAGG CAATGCAGAT AAAAAAAACC TAGTGGATCC TTTTGTAGAA
    GTTTCCTTTG CTGGAAAAAA GGTTTGCACA AATATAATTG AGAAAAATGC AAATCCTGAG
    TGGAACCAAG TCGTTAATCT TCAGATAAAG TTTCCTTCAA TGTGTGAAAA AATAAAACTA
    ACAATATATG ACTGGGACCG TCTTACTAAA AATGATGTTG TTGGAACAAC ATATCTACAC
    CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGATC TCTCATCTTC GGGATCTGGG
    GCTGCGTCAT ATACAGCAAA TACAGGAGAA ACAGAGGTGG GCTTTGTTCC AACATTTGGG
    CCTTGTTATC TGAACCTTTA CGGAAGCCCT AGAGAGTACA CAGGATTCCC AGACCCCTAT
    GATGAGCTAA ATACTGGAAA GGGGGAAGGA GTTGCCTACA GAGGCAGAAT CTTGGTTGAA
    TTAAGCACTT TTCTTGAAAA GACACCACCA GACAAAAAGC TTGAGCCTAT TTCAAATGAT
    GACCTGCTGG TTGTGGAGAA ATACCAACGA AGGCGGAAGT ATAGCCTGTC TGCCGTGTTC
    CATTCGGCCA CCATGTTGCA AGATGTTGGC GAGGCCATTC AGTTTGAAGT CAGCATTGGG
    AACTATGGCA ACAAGTTTGA CACGACTTGT AAGCCTTTGG CATCAACCAC TCAATACAGC
    CGTGCCGTAT TTGATGGGAA CTACTATTAT TACTTGCCTT GGGCCCACAC AAAGCCGGTT
    GTTACCCTGA CTTCATACTG GGAGGACATC AGTCATCGCC TGGATGCAGT GAACACTCTC
    CTAACCATGG CAGAACGGCT GCAATTGAAC TTAGAGGCTC TGAAATCAGG GATGCAAGGT
    AAAGTTCCTG CAAACCAGTT GGCTGAAATA TGGTTGAAGC TGATCGATGA AGTTATAGAA
    GATACAAGGT ACACATTGCC TGTCACGGAA GGAAAAGCTA ATGTCACAAT TCTTGACACT
    CAGATCCGAA AGTTGCGGTC CCGATCTCTC TCCCAGATTC ATGAGGCAGC TGTGAGGATG
    AGGTCTGAGG CCACAGAGGT GAAATCCACA CTGGCAGAAA TTGAGGACTG GCTTGAAAAA
    TTACTGCAAC TGACTGAAGA GCCACAGAAC AGTATGCCTG ACATTATCAT CTGGATGATC
    CGGGGGGAGA AAAGACTGGC CTATGCACGC ATCCCTGCCC ATCAGGTTCT ATACTCCACC
    AGTGGTGAGA CTGCATCTGG GAAATACTGC GGGAAAACCC AAACCATCTT GCTGAAGTAT
    CCGCAGGAAA AAACCCACGG ACCAAAGGTG CCCGCAGAGC TGCGAGTCAA CATCTGGCTG
    GGCTTAAGTG CTGTTGAGAA GAAATTCAAT AGCTTCGCAG AAGGAACATT CACCGTCTTT
    GCTGAAATGT ATGAAAATCA AGCTCTTATG TTTGGAAAAT GGGGCACTTC TGGATTAGTG
    GGTCGTCATA AATTTTCTGA TGTCACAGGA AAAATAAAAC TCAAGAGGGA ATTCTTTTTG
    CCCCCGAAAG GCTGGGAATG GGAAGGAGAG TGGATAGTTG ATCCTGAAAG AAGCTTGCTG
    ACTGAAGCAG ACGCAGGGCA CACGGAGTTC ACGGACGAAG TGTACCAGAA TGAGAGCCGC
    TACCCTGGGG GTGACTGGAA GCCAGCTGAG GACACCTATA CCGATGCGAA TGGTGATAAA
    GCGGCATCAC CTAGCGAGCT GACATGCCCT CCAGGATGGG AATGGGAAGA TGACGCATGG
    GTATATGATA TAAATCGTGC AGTGGATGAG AAAGGCTGGG AGTATGGAAT TACCATTCCT
    CCTGATACTA AACCCAAATC CTGGGTTGCA GCAGAGAAAA TGTATCATAC TCATAGACGA
    CGAAGGCTAG TCCGAAAACG CAAGAAAGAT TTAACACAAA GCACTGCAAG CACTGCAAGG
    GCCATGGAAG AATTGGAAGA CCGGGAGGGC TGGGAATATG CTTCTTTAAT TGGCTGGAAA
    TTTCACTGGA AACAGCGTAG CTCAGATACC TTCCGCCGTA GACGCTGGAG AAGGAAAATG
    GCTCCTTCGG AAACGCACGG TGCGGCCGCC ATCTTTAAAC TGGAGGGCGC CCTCGGTGCA
    GACATGACCG AGGATGGAGA CGAGAAGAGT GTGGACAAGC AGAAGTATAG TGCCACCACT
    GTATTTGGGG CAAACACTCC CATTGTTTCC TGTAACTTTG ACAGAGTCTA CATTTACCAT
    CTGCGCTGCT ATATCTATCA AGCCAGAAAC CTCATGGCTC TAGACAAGGA TAGTTTTTCA
    GATCCATATG CACATGTCTC CTTCCTCCAT CGAAGCAAAA CCACTGAGAT CATCCACTCG
    ACTCTGAATC CCACATGGGA CCAGACAATT ATATTTGATG AAATCGAAAT CTATGGGGAA
    CCCCAGACAG TTCTACAGAA CCCACCCAAA GTTATCATCG AACTTTTTGA CAATGACCAA
    GTGGGCAAAG ATGAATTTTT AGGACGAAGC ATGTGCTCTC CTCTGGTAAA ACTAAACTCA
    GAAACGGATG TCACACCCAA ACTTCTCTGG CACCCTGTAA TGAATGGAGA CAAAGCCTGT
    GGGGATGTCC TTGTAACTGC AGAGCTGATT CTAAGGCACA AGGATGGTTC CAATCTTCCC
    ATCCTTCCCT CACAAAGGGC ACCAAATCTA TACATGGTCC CACAAGGGAT CAGGCCTGTG
    GTCCAACTCA CTGCTATTGA GATTCTAGCT TGGGGCTTAA GAAATATGAA AAACTACCAG
    ATGGCTTCTA TCACCTCCCC CAGCCTCATT GTGGAGTGTG GAGGGGAAAG GGTAGAATCA
    GTGGTGATCA AAAACCTGAA GAAGACACCC AACTTCCCAA GTTCTGTTCT CTTCATGAAA
    GTGTTCTTGC CCAAGGAGGA ACTGTACATG CCTCCGCTGG TGATAAAGGT CATCGACCAC
    AGGCAGTTCG GGCGGAAGCC CGTTGTTGGG CAGTGCACCA TCGATCGTCT GGACCGCTTT
    CGTTGTGACC CTTATGCGGG AAAGGAGGAT CTTGTGCCCC AGCTAAAAGC CTCCCTTCTA
    TCTACCCCAC CCTGCCGGGA TGTCATTATC GAAATAGAAG ACACCAAACC ATTACTGGCT
    TCGAAGCTGA CAGAAAAGGA GGAAGAAATC GTTGACTGGT GGAGTAAATT TTATGCTTCC
    ACAGGAGAAC ATGAAAAATG TGGACAATAT GTTCAGAAAG GATATTCGAA ACTCAAGATA
    TATGATTGTG AACTAGAGGA TGTAACAGAA TTTGAAGGCC TGACAGACTT CTCAGATACT
    TTTAAGCTGT ATCGAGGCAA ATCAGATGAA AATGAAGATC CCTCAGTGGT AGGAGAGTTT
    AAGGGCTCCT TTAGAATCTA CTCTCTGCCG GATGACCCCA GCGTGCCGGC CCCTCCCAGG
    CAGTTTCGGG AATTACCTGA CAGCGTCCCA CAGGAATGCA CGGTTAGGAT TTACATTGTT
    CGAGGCTTAG AGCTCCAGCC CCAGGACAAC AATGGCCTGT GTGACCCTTA TATAAAAATA
    ACACTGGGCA AAAAAGTAAT AGAAGATCGA GACCACTATA TCCCCAACAC CCTCAACCCA
    GTTTTCGGCA GAATGTATGA ACTGAGCTGC TACTTACCTC AAGAAAAAGA TCTGAAAATT
    TCTGTCTATG ACTACGACAC CTTTACCCGT GATGAAAAGG TGGGAGAAAC AATTATTGAT
    CTGGAAAACC GATTCCTTTC CCGGTTTGGG TCTCACTGCG GCATACCTGA GCAGTACTGT
    GTTTCTGGAG TTAACACCTG GCGAGATCAA CTGAGACCAA CTCAGTTGCT TCAAAATGTA
    GCCAGGTTCA AAGGCTTCCC ACCACCCATC CTTTCTGAAG ATGGAAATAG AATCAGATAT
    GGAGGGCGAG ACTACAGTTT GGATGAATTT GAAGCCAACA AAATCCTGCA CCAGCACCTC
    GGGGCCCCCG ATGAGCGTCT GGCCCTTCAC ATCCTCAGGA CTCAGGGGCT GGTCCCCGAG
    CATGTGGAAA CGAGGACTTT GCACAGCACC TTCCAGCCCA ACATTTCCCA GGGGAAACTT
    CAGATGTGGG TGGATGTTTT CCCTAAGAGT TTGGGGCCAC CAGGCCCTCC TTTCAACATC
    ACCCCCAGGA AAGCTAAAAA ATACTACCTG CGTGTGATCA TCTGGAATAC CAAGGACGTT
    CTCTTGGATG AGAAGAGCAT CACAGGAGAG GAAATGAGTG ACATCTATGT CAAAGGCTGG
    ATTCCTGGCA ATGAAGAAAA CAAACAGAAA ACAGATGTCC ATTACAGGTC CTTGGACGGG
    GAAGGGAATT TTAACTGGAG ATTTGTTTTC CCATTTGACT ACCTCCCAGC TGAACAACTC
    TGTATCGTTG CTAAAAAAGA GCATTTCTGG AGTATTGACC AAACTGAATT TCGGGTCCCA
    CCCAGACTGA TCATTCAGAT ATGGGATAAT GACAAGTTTT CTCTGGATGA CTATTTGGGT
    TTCCTGGAAC TTGATTTGCA TCACACCATC ATTCCAGCAA AATCATCAGA GAAATGCAGT
    TTGGATATGA TTCCAGACCT CAAAGCCATG AACCCCCTTA AAGCAAAAAC TGCCTCACTG
    TTTGAGCAGA AGTCCATGAA AGGCTGGTGG CCGTGCTACG CAGATAAAGA TGGCTCCCGC
    GTGATGGCTG GGAAAGTGGA GATGACGTTG GAAATCCTCA ATGAGAAGGA GGCAGACGAG
    AGGCCAGCCG GGAAGGGACG GGATGAACCC AACATGAACC CCAAGCTGGA CCCACCAAAC
    CGACCAGAAA CCTCCTTCCT CTGGTTCACC AACCCATGTA AGACCATGAA GTTCATTGTG
    TGGCGCCGAT TTAAGTGGGT GATCATTGGC CTGCTGATCC TACTGATCCT GCTGCTCTTT
    GTGGCCGTGC TCCTGTACTC CTTACCGAAT TATTTGTCAA TGAAGATTGT GAGGCCAAAT
    GCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_858858.2
    CDS568..6753
    Translation

    Target ORF information:

    RefSeq Version XM_853765.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris myoferlin (MYOF), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_853765.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTGCGGG TGATCGTGGA GTCCGCCAGC AATATCCCGA AAACGAAATT TGGGAAACCG 
    GATCCTATCG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGACAAC
    GAACTCAATC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGAGGGGTAT ACCACTGGAC
    TTTTCATCTT CCCTTACAAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAATTA
    ATTGGCACGG CCACTGTAGC CCTGAAGGAC CTGACTGGTG ACCAGAGCAG ATCACTGCCG
    TACAAACTCA TCTCCCTGCT CAGCGAAAAG GGGCAAGATA CTGGGGCCAC CATTGACTTG
    GTGATTGGCT ATGATCCACC TTCTGCTCCA CATCCAAATG ACCCCAGTGG GACCAGTGTG
    TCAGTCACAG AAGGAGAAGA AGAGGAAGAT GGAGGTGATG AAGACAGGTT GGATGGCACA
    GTCACGGGCC CTGGGCCCAG GGGGTCAGGT GGGATCGTGT CGGAGGCTCA CCTTGCCCGG
    AGGCTCACGA AAGCAAAGAA GAACCGGCGG ATGCTGTCCA ACAAGCCTCA GGACTTCCAG
    ATCCGCGTCC GAGTGATTGA GGGCCGTCAG TTAAGTGGCA ACAACATCAA GCCTGTGGTC
    AAGGTCCATG TCTGTGGCCA GACACACAGA ACAAGGATCA AGAGAGGGAA CAACCCATTT
    TATGATGAGT TATTTTTCTA CAATGTCCAC ATAACCCCTT CTGAACTGAT GGATGAGATC
    ATCAGCATTC GGGTTTATAA TTCCCATTCT TTGCGGGCAG ATTGTCTGAT GGGGGAATTT
    AAGATTGATG TTGGATTCAT TTATGATGAA CCGGGTCACG CTGTCATGAG AAAATGGCTT
    CTTCTCAATG ACCCTGAAGA TGCCAGCTCA GGTGCTAAAG GTTACATGAA AGTCAGCATG
    TTCATCCTGG GAACTGGAGA TGAGCCTCCT GCTGAGAAGC GAGATCGTGA TAATGACAGT
    GATGACGTGG AGAGTAATTT GTTACTCCCT GCTGGCATCG CCCTCCGGTG GGTGACCTTC
    TTGCTTAAAA TCTACCGAGC AGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA
    GTAAAGGAAA TATTTGGAGG CAATGCAGAT AAAAAAAACC TAGTGGATCC TTTTGTAGAA
    GTTTCCTTTG CTGGAAAAAA GGTTTGCACA AATATAATTG AGAAAAATGC AAATCCTGAG
    TGGAACCAAG TCGTTAATCT TCAGATAAAG TTTCCTTCAA TGTGTGAAAA AATAAAACTA
    ACAATATATG ACTGGGACCG TCTTACTAAA AATGATGTTG TTGGAACAAC ATATCTACAC
    CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGATC TCTCATCTTC GGGATCTGGG
    GCTGCGTCAT ATACAGCAAA TACAGGAGAA ACAGAGGTGG GCTTTGTTCC AACATTTGGG
    CCTTGTTATC TGAACCTTTA CGGAAGCCCT AGAGAGTACA CAGGATTCCC AGACCCCTAT
    GATGAGCTAA ATACTGGAAA GGGGGAAGGA GTTGCCTACA GAGGCAGAAT CTTGGTTGAA
    TTAAGCACTT TTCTTGAAAA GACACCACCA GACAAAAAGC TTGAGCCTAT TTCAAATGAT
    GACCTGCTGG TTGTGGAGAA ATACCAACGA AGGCGGAAGT ATAGCCTGTC TGCCGTGTTC
    CATTCGGCCA CCATGTTGCA AGATGTTGGC GAGGCCATTC AGTTTGAAGT CAGCATTGGG
    AACTATGGCA ACAAGTTTGA CACGACTTGT AAGCCTTTGG CATCAACCAC TCAATACAGC
    CGTGCCGTAT TTGATGGGAA CTACTATTAT TACTTGCCTT GGGCCCACAC AAAGCCGGTT
    GTTACCCTGA CTTCATACTG GGAGGACATC AGTCATCGCC TGGATGCAGT GAACACTCTC
    CTAACCATGG CAGAACGGCT GCAATTGAAC TTAGAGGCTC TGAAATCAGG GATGCAAGGT
    AAAGTTCCTG CAAACCAGTT GGCTGAAATA TGGTTGAAGC TGATCGATGA AGTTATAGAA
    GATACAAGGT ACACATTGCC TGTCACGGAA GGAAAAGCTA ATGTCACAAT TCTTGACACT
    CAGATCCGAA AGTTGCGGTC CCGATCTCTC TCCCAGATTC ATGAGGCAGC TGTGAGGATG
    AGGTCTGAGG CCACAGAGGT GAAATCCACA CTGGCAGAAA TTGAGGACTG GCTTGAAAAA
    TTACTGCAAC TGACTGAAGA GCCACAGAAC AGTATGCCTG ACATTATCAT CTGGATGATC
    CGGGGGGAGA AAAGACTGGC CTATGCACGC ATCCCTGCCC ATCAGGTTCT ATACTCCACC
    AGTGGTGAGA CTGCATCTGG GAAATACTGC GGGAAAACCC AAACCATCTT GCTGAAGTAT
    CCGCAGGAAA AAACCCACGG ACCAAAGGTG CCCGCAGAGC TGCGAGTCAA CATCTGGCTG
    GGCTTAAGTG CTGTTGAGAA GAAATTCAAT AGCTTCGCAG AAGGAACATT CACCGTCTTT
    GCTGAAATGT ATGAAAATCA AGCTCTTATG TTTGGAAAAT GGGGCACTTC TGGATTAGTG
    GGTCGTCATA AATTTTCTGA TGTCACAGGA AAAATAAAAC TCAAGAGGGA ATTCTTTTTG
    CCCCCGAAAG GCTGGGAATG GGAAGGAGAG TGGATAGTTG ATCCTGAAAG AAGCTTGCTG
    ACTGAAGCAG ACGCAGGGCA CACGGAGTTC ACGGACGAAG TGTACCAGAA TGAGAGCCGC
    TACCCTGGGG GTGACTGGAA GCCAGCTGAG GACACCTATA CCGATGCGAA TGGTGATAAA
    GCGGCATCAC CTAGCGAGCT GACATGCCCT CCAGGATGGG AATGGGAAGA TGACGCATGG
    GTATATGATA TAAATCGTGC AGTGGATGAG AAAGGCTGGG AGTATGGAAT TACCATTCCT
    CCTGATACTA AACCCAAATC CTGGGTTGCA GCAGAGAAAA TGTATCATAC TCATAGACGA
    CGAAGGCTAG TCCGAAAACG CAAGAAAGAT TTAACACAAA GCACTGCAAG CACTGCAAGG
    GCCATGGAAG AATTGGAAGA CCGGGAGGGC TGGGAATATG CTTCTTTAAT TGGCTGGAAA
    TTTCACTGGA AACAGCGTAG CTCAGATACC TTCCGCCGTA GACGCTGGAG AAGGAAAATG
    GCTCCTTCGG AAACGCACGG TGCGGCCGCC ATCTTTAAAC TGGAGGGCGC CCTCGGTGCA
    GACATGACCG AGGATGGAGA CGAGAAGAGT GTGGACAAGC AGAAGTATAG TGCCACCACT
    GTATTTGGGG CAAACACTCC CATTGTTTCC TGTAACTTTG ACAGAGTCTA CATTTACCAT
    CTGCGCTGCT ATATCTATCA AGCCAGAAAC CTCATGGCTC TAGACAAGGA TAGTTTTTCA
    GATCCATATG CACATGTCTC CTTCCTCCAT CGAAGCAAAA CCACTGAGAT CATCCACTCG
    ACTCTGAATC CCACATGGGA CCAGACAATT ATATTTGATG AAATCGAAAT CTATGGGGAA
    CCCCAGACAG TTCTACAGAA CCCACCCAAA GTTATCATCG AACTTTTTGA CAATGACCAA
    GTGGGCAAAG ATGAATTTTT AGGACGAAGC ATGTGCTCTC CTCTGGTAAA ACTAAACTCA
    GAAACGGATG TCACACCCAA ACTTCTCTGG CACCCTGTAA TGAATGGAGA CAAAGCCTGT
    GGGGATGTCC TTGTAACTGC AGAGCTGATT CTAAGGCACA AGGATGGTTC CAATCTTCCC
    ATCCTTCCCT CACAAAGGGC ACCAAATCTA TACATGGTCC CACAAGGGAT CAGGCCTGTG
    GTCCAACTCA CTGCTATTGA GATTCTAGCT TGGGGCTTAA GAAATATGAA AAACTACCAG
    ATGGCTTCTA TCACCTCCCC CAGCCTCATT GTGGAGTGTG GAGGGGAAAG GGTAGAATCA
    GTGGTGATCA AAAACCTGAA GAAGACACCC AACTTCCCAA GTTCTGTTCT CTTCATGAAA
    GTGTTCTTGC CCAAGGAGGA ACTGTACATG CCTCCGCTGG TGATAAAGGT CATCGACCAC
    AGGCAGTTCG GGCGGAAGCC CGTTGTTGGG CAGTGCACCA TCGATCGTCT GGACCGCTTT
    CGTTGTGACC CTTATGCGGG AAAGGAGGAT CTTGTGCCCC AGCTAAAAGC CTCCCTTCTA
    TCTACCCCAC CCTGCCGGGA TGTCATTATC GAAATAGAAG ACACCAAACC ATTACTGGCT
    TCGAAGCTGA CAGAAAAGGA GGAAGAAATC GTTGACTGGT GGAGTAAATT TTATGCTTCC
    ACAGGAGAAC ATGAAAAATG TGGACAATAT GTTCAGAAAG GATATTCGAA ACTCAAGATA
    TATGATTGTG AACTAGAGGA TGTAACAGAA TTTGAAGGCC TGACAGACTT CTCAGATACT
    TTTAAGCTGT ATCGAGGCAA ATCAGATGAA AATGAAGATC CCTCAGTGGT AGGAGAGTTT
    AAGGGCTCCT TTAGAATCTA CTCTCTGCCG GATGACCCCA GCGTGCCGGC CCCTCCCAGG
    CAGTTTCGGG AATTACCTGA CAGCGTCCCA CAGGAATGCA CGGTTAGGAT TTACATTGTT
    CGAGGCTTAG AGCTCCAGCC CCAGGACAAC AATGGCCTGT GTGACCCTTA TATAAAAATA
    ACACTGGGCA AAAAAGTAAT AGAAGATCGA GACCACTATA TCCCCAACAC CCTCAACCCA
    GTTTTCGGCA GAATGTATGA ACTGAGCTGC TACTTACCTC AAGAAAAAGA TCTGAAAATT
    TCTGTCTATG ACTACGACAC CTTTACCCGT GATGAAAAGG TGGGAGAAAC AATTATTGAT
    CTGGAAAACC GATTCCTTTC CCGGTTTGGG TCTCACTGCG GCATACCTGA GCAGTACTGT
    GTTTCTGGAG TTAACACCTG GCGAGATCAA CTGAGACCAA CTCAGTTGCT TCAAAATGTA
    GCCAGGTTCA AAGGCTTCCC ACCACCCATC CTTTCTGAAG ATGGAAATAG AATCAGATAT
    GGAGGGCGAG ACTACAGTTT GGATGAATTT GAAGCCAACA AAATCCTGCA CCAGCACCTC
    GGGGCCCCCG ATGAGCGTCT GGCCCTTCAC ATCCTCAGGA CTCAGGGGCT GGTCCCCGAG
    CATGTGGAAA CGAGGACTTT GCACAGCACC TTCCAGCCCA ACATTTCCCA GGGGAAACTT
    CAGATGTGGG TGGATGTTTT CCCTAAGAGT TTGGGGCCAC CAGGCCCTCC TTTCAACATC
    ACCCCCAGGA AAGCTAAAAA ATACTACCTG CGTGTGATCA TCTGGAATAC CAAGGACGTT
    CTCTTGGATG AGAAGAGCAT CACAGGAGAG GAAATGAGTG ACATCTATGT CAAAGGCTGG
    ATTCCTGGCA ATGAAGAAAA CAAACAGAAA ACAGATGTCC ATTACAGGTC CTTGGACGGG
    GAAGGGAATT TTAACTGGAG ATTTGTTTTC CCATTTGACT ACCTCCCAGC TGAACAACTC
    TGTATCGTTG CTAAAAAAGA GCATTTCTGG AGTATTGACC AAACTGAATT TCGGGTCCCA
    CCCAGACTGA TCATTCAGAT ATGGGATAAT GACAAGTTTT CTCTGGATGA CTATTTGGGT
    TTCCTGGAAC TTGATTTGCA TCACACCATC ATTCCAGCAA AATCATCAGA GAAATGCAGT
    TTGGATATGA TTCCAGACCT CAAAGCCATG AACCCCCTTA AAGCAAAAAC TGCCTCACTG
    TTTGAGCAGA AGTCCATGAA AGGCTGGTGG CCGTGCTACG CAGATAAAGA TGGCTCCCGC
    GTGATGGCTG GGAAAGTGGA GATGACGTTG GAAATCCTCA ATGAGAAGGA GGCAGACGAG
    AGGCCAGCCG GGAAGGGACG GGATGAACCC AACATGAACC CCAAGCTGGA CCCACCAAAC
    CGACCAGAAA CCTCCTTCCT CTGGTTCACC AACCCATGTA AGACCATGAA GTTCATTGTG
    TGGCGCCGAT TTAAGTGGGT GATCATTGGC CTGCTGATCC TACTGATCCT GCTGCTCTTT
    GTGGCCGTGC TCCTGTACTC CTTACCGAAT TATTTGTCAA TGAAGATTGT GAGGCCAAAT
    GCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27786
    Clone ID Related Accession (Same CDS sequence) XM_853765.4 , XM_853765.5
    Accession Version XM_853765.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6186bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product myoferlin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006610.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_853765.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTGCGGG TGATCGTGGA GTCCGCCAGC AATATCCCGA AAACGAAATT TGGGAAACCG 
    GATCCTATCG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGACAAC
    GAACTCAATC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGAGGGGTAT ACCACTGGAC
    TTTTCATCTT CCCTTACAAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAATTA
    ATTGGCACGG CCACTGTAGC CCTGAAGGAC CTGACTGGTG ACCAGAGCAG ATCACTGCCG
    TACAAACTCA TCTCCCTGCT CAGCGAAAAG GGGCAAGATA CTGGGGCCAC CATTGACTTG
    GTGATTGGCT ATGATCCACC TTCTGCTCCA CATCCAAATG ACCCCAGTGG GACCAGTGTG
    TCAGTCACAG AAGGAGAAGA AGAGGAAGAT GGAGGTGATG AAGACAGGTT GGATGGCACA
    GTCACGGGCC CTGGGCCCAG GGGGTCAGGT GGGATCGTGT CGGAGGCTCA CCTTGCCCGG
    AGGCTCACGA AAGCAAAGAA GAACCGGCGG ATGCTGTCCA ACAAGCCTCA GGACTTCCAG
    ATCCGCGTCC GAGTGATTGA GGGCCGTCAG TTAAGTGGCA ACAACATCAA GCCTGTGGTC
    AAGGTCCATG TCTGTGGCCA GACACACAGA ACAAGGATCA AGAGAGGGAA CAACCCATTT
    TATGATGAGT TATTTTTCTA CAATGTCCAC ATAACCCCTT CTGAACTGAT GGATGAGATC
    ATCAGCATTC GGGTTTATAA TTCCCATTCT TTGCGGGCAG ATTGTCTGAT GGGGGAATTT
    AAGATTGATG TTGGATTCAT TTATGATGAA CCGGGTCACG CTGTCATGAG AAAATGGCTT
    CTTCTCAATG ACCCTGAAGA TGCCAGCTCA GGTGCTAAAG GTTACATGAA AGTCAGCATG
    TTCATCCTGG GAACTGGAGA TGAGCCTCCT GCTGAGAAGC GAGATCGTGA TAATGACAGT
    GATGACGTGG AGAGTAATTT GTTACTCCCT GCTGGCATCG CCCTCCGGTG GGTGACCTTC
    TTGCTTAAAA TCTACCGAGC AGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA
    GTAAAGGAAA TATTTGGAGG CAATGCAGAT AAAAAAAACC TAGTGGATCC TTTTGTAGAA
    GTTTCCTTTG CTGGAAAAAA GGTTTGCACA AATATAATTG AGAAAAATGC AAATCCTGAG
    TGGAACCAAG TCGTTAATCT TCAGATAAAG TTTCCTTCAA TGTGTGAAAA AATAAAACTA
    ACAATATATG ACTGGGACCG TCTTACTAAA AATGATGTTG TTGGAACAAC ATATCTACAC
    CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGATC TCTCATCTTC GGGATCTGGG
    GCTGCGTCAT ATACAGCAAA TACAGGAGAA ACAGAGGTGG GCTTTGTTCC AACATTTGGG
    CCTTGTTATC TGAACCTTTA CGGAAGCCCT AGAGAGTACA CAGGATTCCC AGACCCCTAT
    GATGAGCTAA ATACTGGAAA GGGGGAAGGA GTTGCCTACA GAGGCAGAAT CTTGGTTGAA
    TTAAGCACTT TTCTTGAAAA GACACCACCA GACAAAAAGC TTGAGCCTAT TTCAAATGAT
    GACCTGCTGG TTGTGGAGAA ATACCAACGA AGGCGGAAGT ATAGCCTGTC TGCCGTGTTC
    CATTCGGCCA CCATGTTGCA AGATGTTGGC GAGGCCATTC AGTTTGAAGT CAGCATTGGG
    AACTATGGCA ACAAGTTTGA CACGACTTGT AAGCCTTTGG CATCAACCAC TCAATACAGC
    CGTGCCGTAT TTGATGGGAA CTACTATTAT TACTTGCCTT GGGCCCACAC AAAGCCGGTT
    GTTACCCTGA CTTCATACTG GGAGGACATC AGTCATCGCC TGGATGCAGT GAACACTCTC
    CTAACCATGG CAGAACGGCT GCAATTGAAC TTAGAGGCTC TGAAATCAGG GATGCAAGGT
    AAAGTTCCTG CAAACCAGTT GGCTGAAATA TGGTTGAAGC TGATCGATGA AGTTATAGAA
    GATACAAGGT ACACATTGCC TGTCACGGAA GGAAAAGCTA ATGTCACAAT TCTTGACACT
    CAGATCCGAA AGTTGCGGTC CCGATCTCTC TCCCAGATTC ATGAGGCAGC TGTGAGGATG
    AGGTCTGAGG CCACAGAGGT GAAATCCACA CTGGCAGAAA TTGAGGACTG GCTTGAAAAA
    TTACTGCAAC TGACTGAAGA GCCACAGAAC AGTATGCCTG ACATTATCAT CTGGATGATC
    CGGGGGGAGA AAAGACTGGC CTATGCACGC ATCCCTGCCC ATCAGGTTCT ATACTCCACC
    AGTGGTGAGA CTGCATCTGG GAAATACTGC GGGAAAACCC AAACCATCTT GCTGAAGTAT
    CCGCAGGAAA AAACCCACGG ACCAAAGGTG CCCGCAGAGC TGCGAGTCAA CATCTGGCTG
    GGCTTAAGTG CTGTTGAGAA GAAATTCAAT AGCTTCGCAG AAGGAACATT CACCGTCTTT
    GCTGAAATGT ATGAAAATCA AGCTCTTATG TTTGGAAAAT GGGGCACTTC TGGATTAGTG
    GGTCGTCATA AATTTTCTGA TGTCACAGGA AAAATAAAAC TCAAGAGGGA ATTCTTTTTG
    CCCCCGAAAG GCTGGGAATG GGAAGGAGAG TGGATAGTTG ATCCTGAAAG AAGCTTGCTG
    ACTGAAGCAG ACGCAGGGCA CACGGAGTTC ACGGACGAAG TGTACCAGAA TGAGAGCCGC
    TACCCTGGGG GTGACTGGAA GCCAGCTGAG GACACCTATA CCGATGCGAA TGGTGATAAA
    GCGGCATCAC CTAGCGAGCT GACATGCCCT CCAGGATGGG AATGGGAAGA TGACGCATGG
    GTATATGATA TAAATCGTGC AGTGGATGAG AAAGGCTGGG AGTATGGAAT TACCATTCCT
    CCTGATACTA AACCCAAATC CTGGGTTGCA GCAGAGAAAA TGTATCATAC TCATAGACGA
    CGAAGGCTAG TCCGAAAACG CAAGAAAGAT TTAACACAAA GCACTGCAAG CACTGCAAGG
    GCCATGGAAG AATTGGAAGA CCGGGAGGGC TGGGAATATG CTTCTTTAAT TGGCTGGAAA
    TTTCACTGGA AACAGCGTAG CTCAGATACC TTCCGCCGTA GACGCTGGAG AAGGAAAATG
    GCTCCTTCGG AAACGCACGG TGCGGCCGCC ATCTTTAAAC TGGAGGGCGC CCTCGGTGCA
    GACATGACCG AGGATGGAGA CGAGAAGAGT GTGGACAAGC AGAAGTATAG TGCCACCACT
    GTATTTGGGG CAAACACTCC CATTGTTTCC TGTAACTTTG ACAGAGTCTA CATTTACCAT
    CTGCGCTGCT ATATCTATCA AGCCAGAAAC CTCATGGCTC TAGACAAGGA TAGTTTTTCA
    GATCCATATG CACATGTCTC CTTCCTCCAT CGAAGCAAAA CCACTGAGAT CATCCACTCG
    ACTCTGAATC CCACATGGGA CCAGACAATT ATATTTGATG AAATCGAAAT CTATGGGGAA
    CCCCAGACAG TTCTACAGAA CCCACCCAAA GTTATCATCG AACTTTTTGA CAATGACCAA
    GTGGGCAAAG ATGAATTTTT AGGACGAAGC ATGTGCTCTC CTCTGGTAAA ACTAAACTCA
    GAAACGGATG TCACACCCAA ACTTCTCTGG CACCCTGTAA TGAATGGAGA CAAAGCCTGT
    GGGGATGTCC TTGTAACTGC AGAGCTGATT CTAAGGCACA AGGATGGTTC CAATCTTCCC
    ATCCTTCCCT CACAAAGGGC ACCAAATCTA TACATGGTCC CACAAGGGAT CAGGCCTGTG
    GTCCAACTCA CTGCTATTGA GATTCTAGCT TGGGGCTTAA GAAATATGAA AAACTACCAG
    ATGGCTTCTA TCACCTCCCC CAGCCTCATT GTGGAGTGTG GAGGGGAAAG GGTAGAATCA
    GTGGTGATCA AAAACCTGAA GAAGACACCC AACTTCCCAA GTTCTGTTCT CTTCATGAAA
    GTGTTCTTGC CCAAGGAGGA ACTGTACATG CCTCCGCTGG TGATAAAGGT CATCGACCAC
    AGGCAGTTCG GGCGGAAGCC CGTTGTTGGG CAGTGCACCA TCGATCGTCT GGACCGCTTT
    CGTTGTGACC CTTATGCGGG AAAGGAGGAT CTTGTGCCCC AGCTAAAAGC CTCCCTTCTA
    TCTACCCCAC CCTGCCGGGA TGTCATTATC GAAATAGAAG ACACCAAACC ATTACTGGCT
    TCGAAGCTGA CAGAAAAGGA GGAAGAAATC GTTGACTGGT GGAGTAAATT TTATGCTTCC
    ACAGGAGAAC ATGAAAAATG TGGACAATAT GTTCAGAAAG GATATTCGAA ACTCAAGATA
    TATGATTGTG AACTAGAGGA TGTAACAGAA TTTGAAGGCC TGACAGACTT CTCAGATACT
    TTTAAGCTGT ATCGAGGCAA ATCAGATGAA AATGAAGATC CCTCAGTGGT AGGAGAGTTT
    AAGGGCTCCT TTAGAATCTA CTCTCTGCCG GATGACCCCA GCGTGCCGGC CCCTCCCAGG
    CAGTTTCGGG AATTACCTGA CAGCGTCCCA CAGGAATGCA CGGTTAGGAT TTACATTGTT
    CGAGGCTTAG AGCTCCAGCC CCAGGACAAC AATGGCCTGT GTGACCCTTA TATAAAAATA
    ACACTGGGCA AAAAAGTAAT AGAAGATCGA GACCACTATA TCCCCAACAC CCTCAACCCA
    GTTTTCGGCA GAATGTATGA ACTGAGCTGC TACTTACCTC AAGAAAAAGA TCTGAAAATT
    TCTGTCTATG ACTACGACAC CTTTACCCGT GATGAAAAGG TGGGAGAAAC AATTATTGAT
    CTGGAAAACC GATTCCTTTC CCGGTTTGGG TCTCACTGCG GCATACCTGA GCAGTACTGT
    GTTTCTGGAG TTAACACCTG GCGAGATCAA CTGAGACCAA CTCAGTTGCT TCAAAATGTA
    GCCAGGTTCA AAGGCTTCCC ACCACCCATC CTTTCTGAAG ATGGAAATAG AATCAGATAT
    GGAGGGCGAG ACTACAGTTT GGATGAATTT GAAGCCAACA AAATCCTGCA CCAGCACCTC
    GGGGCCCCCG ATGAGCGTCT GGCCCTTCAC ATCCTCAGGA CTCAGGGGCT GGTCCCCGAG
    CATGTGGAAA CGAGGACTTT GCACAGCACC TTCCAGCCCA ACATTTCCCA GGGGAAACTT
    CAGATGTGGG TGGATGTTTT CCCTAAGAGT TTGGGGCCAC CAGGCCCTCC TTTCAACATC
    ACCCCCAGGA AAGCTAAAAA ATACTACCTG CGTGTGATCA TCTGGAATAC CAAGGACGTT
    CTCTTGGATG AGAAGAGCAT CACAGGAGAG GAAATGAGTG ACATCTATGT CAAAGGCTGG
    ATTCCTGGCA ATGAAGAAAA CAAACAGAAA ACAGATGTCC ATTACAGGTC CTTGGACGGG
    GAAGGGAATT TTAACTGGAG ATTTGTTTTC CCATTTGACT ACCTCCCAGC TGAACAACTC
    TGTATCGTTG CTAAAAAAGA GCATTTCTGG AGTATTGACC AAACTGAATT TCGGGTCCCA
    CCCAGACTGA TCATTCAGAT ATGGGATAAT GACAAGTTTT CTCTGGATGA CTATTTGGGT
    TTCCTGGAAC TTGATTTGCA TCACACCATC ATTCCAGCAA AATCATCAGA GAAATGCAGT
    TTGGATATGA TTCCAGACCT CAAAGCCATG AACCCCCTTA AAGCAAAAAC TGCCTCACTG
    TTTGAGCAGA AGTCCATGAA AGGCTGGTGG CCGTGCTACG CAGATAAAGA TGGCTCCCGC
    GTGATGGCTG GGAAAGTGGA GATGACGTTG GAAATCCTCA ATGAGAAGGA GGCAGACGAG
    AGGCCAGCCG GGAAGGGACG GGATGAACCC AACATGAACC CCAAGCTGGA CCCACCAAAC
    CGACCAGAAA CCTCCTTCCT CTGGTTCACC AACCCATGTA AGACCATGAA GTTCATTGTG
    TGGCGCCGAT TTAAGTGGGT GATCATTGGC CTGCTGATCC TACTGATCCT GCTGCTCTTT
    GTGGCCGTGC TCCTGTACTC CTTACCGAAT TATTTGTCAA TGAAGATTGT GAGGCCAAAT
    GCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_858858.2
    CDS120..6305
    Translation

    Target ORF information:

    RefSeq Version XM_853765.5
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris myoferlin (MYOF), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_853765.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTGCGGG TGATCGTGGA GTCCGCCAGC AATATCCCGA AAACGAAATT TGGGAAACCG 
    GATCCTATCG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGACAAC
    GAACTCAATC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGAGGGGTAT ACCACTGGAC
    TTTTCATCTT CCCTTACAAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAATTA
    ATTGGCACGG CCACTGTAGC CCTGAAGGAC CTGACTGGTG ACCAGAGCAG ATCACTGCCG
    TACAAACTCA TCTCCCTGCT CAGCGAAAAG GGGCAAGATA CTGGGGCCAC CATTGACTTG
    GTGATTGGCT ATGATCCACC TTCTGCTCCA CATCCAAATG ACCCCAGTGG GACCAGTGTG
    TCAGTCACAG AAGGAGAAGA AGAGGAAGAT GGAGGTGATG AAGACAGGTT GGATGGCACA
    GTCACGGGCC CTGGGCCCAG GGGGTCAGGT GGGATCGTGT CGGAGGCTCA CCTTGCCCGG
    AGGCTCACGA AAGCAAAGAA GAACCGGCGG ATGCTGTCCA ACAAGCCTCA GGACTTCCAG
    ATCCGCGTCC GAGTGATTGA GGGCCGTCAG TTAAGTGGCA ACAACATCAA GCCTGTGGTC
    AAGGTCCATG TCTGTGGCCA GACACACAGA ACAAGGATCA AGAGAGGGAA CAACCCATTT
    TATGATGAGT TATTTTTCTA CAATGTCCAC ATAACCCCTT CTGAACTGAT GGATGAGATC
    ATCAGCATTC GGGTTTATAA TTCCCATTCT TTGCGGGCAG ATTGTCTGAT GGGGGAATTT
    AAGATTGATG TTGGATTCAT TTATGATGAA CCGGGTCACG CTGTCATGAG AAAATGGCTT
    CTTCTCAATG ACCCTGAAGA TGCCAGCTCA GGTGCTAAAG GTTACATGAA AGTCAGCATG
    TTCATCCTGG GAACTGGAGA TGAGCCTCCT GCTGAGAAGC GAGATCGTGA TAATGACAGT
    GATGACGTGG AGAGTAATTT GTTACTCCCT GCTGGCATCG CCCTCCGGTG GGTGACCTTC
    TTGCTTAAAA TCTACCGAGC AGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA
    GTAAAGGAAA TATTTGGAGG CAATGCAGAT AAAAAAAACC TAGTGGATCC TTTTGTAGAA
    GTTTCCTTTG CTGGAAAAAA GGTTTGCACA AATATAATTG AGAAAAATGC AAATCCTGAG
    TGGAACCAAG TCGTTAATCT TCAGATAAAG TTTCCTTCAA TGTGTGAAAA AATAAAACTA
    ACAATATATG ACTGGGACCG TCTTACTAAA AATGATGTTG TTGGAACAAC ATATCTACAC
    CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGATC TCTCATCTTC GGGATCTGGG
    GCTGCGTCAT ATACAGCAAA TACAGGAGAA ACAGAGGTGG GCTTTGTTCC AACATTTGGG
    CCTTGTTATC TGAACCTTTA CGGAAGCCCT AGAGAGTACA CAGGATTCCC AGACCCCTAT
    GATGAGCTAA ATACTGGAAA GGGGGAAGGA GTTGCCTACA GAGGCAGAAT CTTGGTTGAA
    TTAAGCACTT TTCTTGAAAA GACACCACCA GACAAAAAGC TTGAGCCTAT TTCAAATGAT
    GACCTGCTGG TTGTGGAGAA ATACCAACGA AGGCGGAAGT ATAGCCTGTC TGCCGTGTTC
    CATTCGGCCA CCATGTTGCA AGATGTTGGC GAGGCCATTC AGTTTGAAGT CAGCATTGGG
    AACTATGGCA ACAAGTTTGA CACGACTTGT AAGCCTTTGG CATCAACCAC TCAATACAGC
    CGTGCCGTAT TTGATGGGAA CTACTATTAT TACTTGCCTT GGGCCCACAC AAAGCCGGTT
    GTTACCCTGA CTTCATACTG GGAGGACATC AGTCATCGCC TGGATGCAGT GAACACTCTC
    CTAACCATGG CAGAACGGCT GCAATTGAAC TTAGAGGCTC TGAAATCAGG GATGCAAGGT
    AAAGTTCCTG CAAACCAGTT GGCTGAAATA TGGTTGAAGC TGATCGATGA AGTTATAGAA
    GATACAAGGT ACACATTGCC TGTCACGGAA GGAAAAGCTA ATGTCACAAT TCTTGACACT
    CAGATCCGAA AGTTGCGGTC CCGATCTCTC TCCCAGATTC ATGAGGCAGC TGTGAGGATG
    AGGTCTGAGG CCACAGAGGT GAAATCCACA CTGGCAGAAA TTGAGGACTG GCTTGAAAAA
    TTACTGCAAC TGACTGAAGA GCCACAGAAC AGTATGCCTG ACATTATCAT CTGGATGATC
    CGGGGGGAGA AAAGACTGGC CTATGCACGC ATCCCTGCCC ATCAGGTTCT ATACTCCACC
    AGTGGTGAGA CTGCATCTGG GAAATACTGC GGGAAAACCC AAACCATCTT GCTGAAGTAT
    CCGCAGGAAA AAACCCACGG ACCAAAGGTG CCCGCAGAGC TGCGAGTCAA CATCTGGCTG
    GGCTTAAGTG CTGTTGAGAA GAAATTCAAT AGCTTCGCAG AAGGAACATT CACCGTCTTT
    GCTGAAATGT ATGAAAATCA AGCTCTTATG TTTGGAAAAT GGGGCACTTC TGGATTAGTG
    GGTCGTCATA AATTTTCTGA TGTCACAGGA AAAATAAAAC TCAAGAGGGA ATTCTTTTTG
    CCCCCGAAAG GCTGGGAATG GGAAGGAGAG TGGATAGTTG ATCCTGAAAG AAGCTTGCTG
    ACTGAAGCAG ACGCAGGGCA CACGGAGTTC ACGGACGAAG TGTACCAGAA TGAGAGCCGC
    TACCCTGGGG GTGACTGGAA GCCAGCTGAG GACACCTATA CCGATGCGAA TGGTGATAAA
    GCGGCATCAC CTAGCGAGCT GACATGCCCT CCAGGATGGG AATGGGAAGA TGACGCATGG
    GTATATGATA TAAATCGTGC AGTGGATGAG AAAGGCTGGG AGTATGGAAT TACCATTCCT
    CCTGATACTA AACCCAAATC CTGGGTTGCA GCAGAGAAAA TGTATCATAC TCATAGACGA
    CGAAGGCTAG TCCGAAAACG CAAGAAAGAT TTAACACAAA GCACTGCAAG CACTGCAAGG
    GCCATGGAAG AATTGGAAGA CCGGGAGGGC TGGGAATATG CTTCTTTAAT TGGCTGGAAA
    TTTCACTGGA AACAGCGTAG CTCAGATACC TTCCGCCGTA GACGCTGGAG AAGGAAAATG
    GCTCCTTCGG AAACGCACGG TGCGGCCGCC ATCTTTAAAC TGGAGGGCGC CCTCGGTGCA
    GACATGACCG AGGATGGAGA CGAGAAGAGT GTGGACAAGC AGAAGTATAG TGCCACCACT
    GTATTTGGGG CAAACACTCC CATTGTTTCC TGTAACTTTG ACAGAGTCTA CATTTACCAT
    CTGCGCTGCT ATATCTATCA AGCCAGAAAC CTCATGGCTC TAGACAAGGA TAGTTTTTCA
    GATCCATATG CACATGTCTC CTTCCTCCAT CGAAGCAAAA CCACTGAGAT CATCCACTCG
    ACTCTGAATC CCACATGGGA CCAGACAATT ATATTTGATG AAATCGAAAT CTATGGGGAA
    CCCCAGACAG TTCTACAGAA CCCACCCAAA GTTATCATCG AACTTTTTGA CAATGACCAA
    GTGGGCAAAG ATGAATTTTT AGGACGAAGC ATGTGCTCTC CTCTGGTAAA ACTAAACTCA
    GAAACGGATG TCACACCCAA ACTTCTCTGG CACCCTGTAA TGAATGGAGA CAAAGCCTGT
    GGGGATGTCC TTGTAACTGC AGAGCTGATT CTAAGGCACA AGGATGGTTC CAATCTTCCC
    ATCCTTCCCT CACAAAGGGC ACCAAATCTA TACATGGTCC CACAAGGGAT CAGGCCTGTG
    GTCCAACTCA CTGCTATTGA GATTCTAGCT TGGGGCTTAA GAAATATGAA AAACTACCAG
    ATGGCTTCTA TCACCTCCCC CAGCCTCATT GTGGAGTGTG GAGGGGAAAG GGTAGAATCA
    GTGGTGATCA AAAACCTGAA GAAGACACCC AACTTCCCAA GTTCTGTTCT CTTCATGAAA
    GTGTTCTTGC CCAAGGAGGA ACTGTACATG CCTCCGCTGG TGATAAAGGT CATCGACCAC
    AGGCAGTTCG GGCGGAAGCC CGTTGTTGGG CAGTGCACCA TCGATCGTCT GGACCGCTTT
    CGTTGTGACC CTTATGCGGG AAAGGAGGAT CTTGTGCCCC AGCTAAAAGC CTCCCTTCTA
    TCTACCCCAC CCTGCCGGGA TGTCATTATC GAAATAGAAG ACACCAAACC ATTACTGGCT
    TCGAAGCTGA CAGAAAAGGA GGAAGAAATC GTTGACTGGT GGAGTAAATT TTATGCTTCC
    ACAGGAGAAC ATGAAAAATG TGGACAATAT GTTCAGAAAG GATATTCGAA ACTCAAGATA
    TATGATTGTG AACTAGAGGA TGTAACAGAA TTTGAAGGCC TGACAGACTT CTCAGATACT
    TTTAAGCTGT ATCGAGGCAA ATCAGATGAA AATGAAGATC CCTCAGTGGT AGGAGAGTTT
    AAGGGCTCCT TTAGAATCTA CTCTCTGCCG GATGACCCCA GCGTGCCGGC CCCTCCCAGG
    CAGTTTCGGG AATTACCTGA CAGCGTCCCA CAGGAATGCA CGGTTAGGAT TTACATTGTT
    CGAGGCTTAG AGCTCCAGCC CCAGGACAAC AATGGCCTGT GTGACCCTTA TATAAAAATA
    ACACTGGGCA AAAAAGTAAT AGAAGATCGA GACCACTATA TCCCCAACAC CCTCAACCCA
    GTTTTCGGCA GAATGTATGA ACTGAGCTGC TACTTACCTC AAGAAAAAGA TCTGAAAATT
    TCTGTCTATG ACTACGACAC CTTTACCCGT GATGAAAAGG TGGGAGAAAC AATTATTGAT
    CTGGAAAACC GATTCCTTTC CCGGTTTGGG TCTCACTGCG GCATACCTGA GCAGTACTGT
    GTTTCTGGAG TTAACACCTG GCGAGATCAA CTGAGACCAA CTCAGTTGCT TCAAAATGTA
    GCCAGGTTCA AAGGCTTCCC ACCACCCATC CTTTCTGAAG ATGGAAATAG AATCAGATAT
    GGAGGGCGAG ACTACAGTTT GGATGAATTT GAAGCCAACA AAATCCTGCA CCAGCACCTC
    GGGGCCCCCG ATGAGCGTCT GGCCCTTCAC ATCCTCAGGA CTCAGGGGCT GGTCCCCGAG
    CATGTGGAAA CGAGGACTTT GCACAGCACC TTCCAGCCCA ACATTTCCCA GGGGAAACTT
    CAGATGTGGG TGGATGTTTT CCCTAAGAGT TTGGGGCCAC CAGGCCCTCC TTTCAACATC
    ACCCCCAGGA AAGCTAAAAA ATACTACCTG CGTGTGATCA TCTGGAATAC CAAGGACGTT
    CTCTTGGATG AGAAGAGCAT CACAGGAGAG GAAATGAGTG ACATCTATGT CAAAGGCTGG
    ATTCCTGGCA ATGAAGAAAA CAAACAGAAA ACAGATGTCC ATTACAGGTC CTTGGACGGG
    GAAGGGAATT TTAACTGGAG ATTTGTTTTC CCATTTGACT ACCTCCCAGC TGAACAACTC
    TGTATCGTTG CTAAAAAAGA GCATTTCTGG AGTATTGACC AAACTGAATT TCGGGTCCCA
    CCCAGACTGA TCATTCAGAT ATGGGATAAT GACAAGTTTT CTCTGGATGA CTATTTGGGT
    TTCCTGGAAC TTGATTTGCA TCACACCATC ATTCCAGCAA AATCATCAGA GAAATGCAGT
    TTGGATATGA TTCCAGACCT CAAAGCCATG AACCCCCTTA AAGCAAAAAC TGCCTCACTG
    TTTGAGCAGA AGTCCATGAA AGGCTGGTGG CCGTGCTACG CAGATAAAGA TGGCTCCCGC
    GTGATGGCTG GGAAAGTGGA GATGACGTTG GAAATCCTCA ATGAGAAGGA GGCAGACGAG
    AGGCCAGCCG GGAAGGGACG GGATGAACCC AACATGAACC CCAAGCTGGA CCCACCAAAC
    CGACCAGAAA CCTCCTTCCT CTGGTTCACC AACCCATGTA AGACCATGAA GTTCATTGTG
    TGGCGCCGAT TTAAGTGGGT GATCATTGGC CTGCTGATCC TACTGATCCT GCTGCTCTTT
    GTGGCCGTGC TCCTGTACTC CTTACCGAAT TATTTGTCAA TGAAGATTGT GAGGCCAAAT
    GCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27787
    Clone ID Related Accession (Same CDS sequence) XM_005637544.3 , XM_005637544.2
    Accession Version XM_005637544.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6147bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product myoferlin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006610.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005637544.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGCTGCGGG TGATCGTGGA GTCCGCCAGC AATATCCCGA AAACGAAATT TGGGAAACCG 
    GATCCTATCG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGACAAC
    GAACTCAATC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGAGGGGTAT ACCACTGGAC
    TTTTCATCTT CCCTTACAAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAATTA
    ATTGGCACGG CCACTGTAGC CCTGAAGGAC CTGACTGGTG ACCAGAGCAG ATCACTGCCG
    TACAAACTCA TCTCCCTGCT CAGCGAAAAG GGGCAAGATA CTGGGGCCAC CATTGACTTG
    GTGATTGGCT ATGATCCACC TTCTGCTCCA CATCCAAATG ACCCCAGTGG GACCAGTGTG
    TCAGTCACAG AAGGAGAAGA AGAGGAAGAT GGAGGTGATG AAGACAGGTT GGATGGCACA
    GTCACGGGCC CTGGGCCCAG GGGGTCAGGT GGGATCGTGT CGGAGGCTCA CCTTGCCCGG
    AGGCTCACGA AAGCAAAGAA GAACCGGCGG ATGCTGTCCA ACAAGCCTCA GGACTTCCAG
    ATCCGCGTCC GAGTGATTGA GGGCCGTCAG TTAAGTGGCA ACAACATCAA GCCTGTGGTC
    AAGGTCCATG TCTGTGGCCA GACACACAGA ACAAGGATCA AGAGAGGGAA CAACCCATTT
    TATGATGAGT TATTTTTCTA CAATGTCCAC ATAACCCCTT CTGAACTGAT GGATGAGATC
    ATCAGCATTC GGGTTTATAA TTCCCATTCT TTGCGGGCAG ATTGTCTGAT GGGGGAATTT
    AAGATTGATG TTGGATTCAT TTATGATGAA CCGGGTCACG CTGTCATGAG AAAATGGCTT
    CTTCTCAATG ACCCTGAAGA TGCCAGCTCA GGTGCTAAAG GTTACATGAA AGTCAGCATG
    TTCATCCTGG GAACTGGAGA TGAGCCTCCT GCTGAGAAGC GAGATCGTGA TAATGACAGT
    GATGACGTGG AGAGTAATTT GTTACTCCCT GCTGGCATCG CCCTCCGGTG GGTGACCTTC
    TTGCTTAAAA TCTACCGAGC AGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA
    GTAAAGGAAA TATTTGGAGG CAATGCAGAT AAAAAAAACC TAGTGGATCC TTTTGTAGAA
    GTTTCCTTTG CTGGAAAAAA GGTTTGCACA AATATAATTG AGAAAAATGC AAATCCTGAG
    TGGAACCAAG TCGTTAATCT TCAGATAAAG TTTCCTTCAA TGTGTGAAAA AATAAAACTA
    ACAATATATG ACTGGGACCG TCTTACTAAA AATGATGTTG TTGGAACAAC ATATCTACAC
    CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGCAA ATACAGGAGA AACAGAGGTG
    GGCTTTGTTC CAACATTTGG GCCTTGTTAT CTGAACCTTT ACGGAAGCCC TAGAGAGTAC
    ACAGGATTCC CAGACCCCTA TGATGAGCTA AATACTGGAA AGGGGGAAGG AGTTGCCTAC
    AGAGGCAGAA TCTTGGTTGA ATTAAGCACT TTTCTTGAAA AGACACCACC AGACAAAAAG
    CTTGAGCCTA TTTCAAATGA TGACCTGCTG GTTGTGGAGA AATACCAACG AAGGCGGAAG
    TATAGCCTGT CTGCCGTGTT CCATTCGGCC ACCATGTTGC AAGATGTTGG CGAGGCCATT
    CAGTTTGAAG TCAGCATTGG GAACTATGGC AACAAGTTTG ACACGACTTG TAAGCCTTTG
    GCATCAACCA CTCAATACAG CCGTGCCGTA TTTGATGGGA ACTACTATTA TTACTTGCCT
    TGGGCCCACA CAAAGCCGGT TGTTACCCTG ACTTCATACT GGGAGGACAT CAGTCATCGC
    CTGGATGCAG TGAACACTCT CCTAACCATG GCAGAACGGC TGCAATTGAA CTTAGAGGCT
    CTGAAATCAG GGATGCAAGG TAAAGTTCCT GCAAACCAGT TGGCTGAAAT ATGGTTGAAG
    CTGATCGATG AAGTTATAGA AGATACAAGG TACACATTGC CTGTCACGGA AGGAAAAGCT
    AATGTCACAA TTCTTGACAC TCAGATCCGA AAGTTGCGGT CCCGATCTCT CTCCCAGATT
    CATGAGGCAG CTGTGAGGAT GAGGTCTGAG GCCACAGAGG TGAAATCCAC ACTGGCAGAA
    ATTGAGGACT GGCTTGAAAA ATTACTGCAA CTGACTGAAG AGCCACAGAA CAGTATGCCT
    GACATTATCA TCTGGATGAT CCGGGGGGAG AAAAGACTGG CCTATGCACG CATCCCTGCC
    CATCAGGTTC TATACTCCAC CAGTGGTGAG ACTGCATCTG GGAAATACTG CGGGAAAACC
    CAAACCATCT TGCTGAAGTA TCCGCAGGAA AAAACCCACG GACCAAAGGT GCCCGCAGAG
    CTGCGAGTCA ACATCTGGCT GGGCTTAAGT GCTGTTGAGA AGAAATTCAA TAGCTTCGCA
    GAAGGAACAT TCACCGTCTT TGCTGAAATG TATGAAAATC AAGCTCTTAT GTTTGGAAAA
    TGGGGCACTT CTGGATTAGT GGGTCGTCAT AAATTTTCTG ATGTCACAGG AAAAATAAAA
    CTCAAGAGGG AATTCTTTTT GCCCCCGAAA GGCTGGGAAT GGGAAGGAGA GTGGATAGTT
    GATCCTGAAA GAAGCTTGCT GACTGAAGCA GACGCAGGGC ACACGGAGTT CACGGACGAA
    GTGTACCAGA ATGAGAGCCG CTACCCTGGG GGTGACTGGA AGCCAGCTGA GGACACCTAT
    ACCGATGCGA ATGGTGATAA AGCGGCATCA CCTAGCGAGC TGACATGCCC TCCAGGATGG
    GAATGGGAAG ATGACGCATG GGTATATGAT ATAAATCGTG CAGTGGATGA GAAAGGCTGG
    GAGTATGGAA TTACCATTCC TCCTGATACT AAACCCAAAT CCTGGGTTGC AGCAGAGAAA
    ATGTATCATA CTCATAGACG ACGAAGGCTA GTCCGAAAAC GCAAGAAAGA TTTAACACAA
    AGCACTGCAA GCACTGCAAG GGCCATGGAA GAATTGGAAG ACCGGGAGGG CTGGGAATAT
    GCTTCTTTAA TTGGCTGGAA ATTTCACTGG AAACAGCGTA GCTCAGATAC CTTCCGCCGT
    AGACGCTGGA GAAGGAAAAT GGCTCCTTCG GAAACGCACG GTGCGGCCGC CATCTTTAAA
    CTGGAGGGCG CCCTCGGTGC AGACATGACC GAGGATGGAG ACGAGAAGAG TGTGGACAAG
    CAGAAGTATA GTGCCACCAC TGTATTTGGG GCAAACACTC CCATTGTTTC CTGTAACTTT
    GACAGAGTCT ACATTTACCA TCTGCGCTGC TATATCTATC AAGCCAGAAA CCTCATGGCT
    CTAGACAAGG ATAGTTTTTC AGATCCATAT GCACATGTCT CCTTCCTCCA TCGAAGCAAA
    ACCACTGAGA TCATCCACTC GACTCTGAAT CCCACATGGG ACCAGACAAT TATATTTGAT
    GAAATCGAAA TCTATGGGGA ACCCCAGACA GTTCTACAGA ACCCACCCAA AGTTATCATC
    GAACTTTTTG ACAATGACCA AGTGGGCAAA GATGAATTTT TAGGACGAAG CATGTGCTCT
    CCTCTGGTAA AACTAAACTC AGAAACGGAT GTCACACCCA AACTTCTCTG GCACCCTGTA
    ATGAATGGAG ACAAAGCCTG TGGGGATGTC CTTGTAACTG CAGAGCTGAT TCTAAGGCAC
    AAGGATGGTT CCAATCTTCC CATCCTTCCC TCACAAAGGG CACCAAATCT ATACATGGTC
    CCACAAGGGA TCAGGCCTGT GGTCCAACTC ACTGCTATTG AGATTCTAGC TTGGGGCTTA
    AGAAATATGA AAAACTACCA GATGGCTTCT ATCACCTCCC CCAGCCTCAT TGTGGAGTGT
    GGAGGGGAAA GGGTAGAATC AGTGGTGATC AAAAACCTGA AGAAGACACC CAACTTCCCA
    AGTTCTGTTC TCTTCATGAA AGTGTTCTTG CCCAAGGAGG AACTGTACAT GCCTCCGCTG
    GTGATAAAGG TCATCGACCA CAGGCAGTTC GGGCGGAAGC CCGTTGTTGG GCAGTGCACC
    ATCGATCGTC TGGACCGCTT TCGTTGTGAC CCTTATGCGG GAAAGGAGGA TCTTGTGCCC
    CAGCTAAAAG CCTCCCTTCT ATCTACCCCA CCCTGCCGGG ATGTCATTAT CGAAATAGAA
    GACACCAAAC CATTACTGGC TTCGAAGCTG ACAGAAAAGG AGGAAGAAAT CGTTGACTGG
    TGGAGTAAAT TTTATGCTTC CACAGGAGAA CATGAAAAAT GTGGACAATA TGTTCAGAAA
    GGATATTCGA AACTCAAGAT ATATGATTGT GAACTAGAGG ATGTAACAGA ATTTGAAGGC
    CTGACAGACT TCTCAGATAC TTTTAAGCTG TATCGAGGCA AATCAGATGA AAATGAAGAT
    CCCTCAGTGG TAGGAGAGTT TAAGGGCTCC TTTAGAATCT ACTCTCTGCC GGATGACCCC
    AGCGTGCCGG CCCCTCCCAG GCAGTTTCGG GAATTACCTG ACAGCGTCCC ACAGGAATGC
    ACGGTTAGGA TTTACATTGT TCGAGGCTTA GAGCTCCAGC CCCAGGACAA CAATGGCCTG
    TGTGACCCTT ATATAAAAAT AACACTGGGC AAAAAAGTAA TAGAAGATCG AGACCACTAT
    ATCCCCAACA CCCTCAACCC AGTTTTCGGC AGAATGTATG AACTGAGCTG CTACTTACCT
    CAAGAAAAAG ATCTGAAAAT TTCTGTCTAT GACTACGACA CCTTTACCCG TGATGAAAAG
    GTGGGAGAAA CAATTATTGA TCTGGAAAAC CGATTCCTTT CCCGGTTTGG GTCTCACTGC
    GGCATACCTG AGCAGTACTG TGTTTCTGGA GTTAACACCT GGCGAGATCA ACTGAGACCA
    ACTCAGTTGC TTCAAAATGT AGCCAGGTTC AAAGGCTTCC CACCACCCAT CCTTTCTGAA
    GATGGAAATA GAATCAGATA TGGAGGGCGA GACTACAGTT TGGATGAATT TGAAGCCAAC
    AAAATCCTGC ACCAGCACCT CGGGGCCCCC GATGAGCGTC TGGCCCTTCA CATCCTCAGG
    ACTCAGGGGC TGGTCCCCGA GCATGTGGAA ACGAGGACTT TGCACAGCAC CTTCCAGCCC
    AACATTTCCC AGGGGAAACT TCAGATGTGG GTGGATGTTT TCCCTAAGAG TTTGGGGCCA
    CCAGGCCCTC CTTTCAACAT CACCCCCAGG AAAGCTAAAA AATACTACCT GCGTGTGATC
    ATCTGGAATA CCAAGGACGT TCTCTTGGAT GAGAAGAGCA TCACAGGAGA GGAAATGAGT
    GACATCTATG TCAAAGGCTG GATTCCTGGC AATGAAGAAA ACAAACAGAA AACAGATGTC
    CATTACAGGT CCTTGGACGG GGAAGGGAAT TTTAACTGGA GATTTGTTTT CCCATTTGAC
    TACCTCCCAG CTGAACAACT CTGTATCGTT GCTAAAAAAG AGCATTTCTG GAGTATTGAC
    CAAACTGAAT TTCGGGTCCC ACCCAGACTG ATCATTCAGA TATGGGATAA TGACAAGTTT
    TCTCTGGATG ACTATTTGGG TTTCCTGGAA CTTGATTTGC ATCACACCAT CATTCCAGCA
    AAATCATCAG AGAAATGCAG TTTGGATATG ATTCCAGACC TCAAAGCCAT GAACCCCCTT
    AAAGCAAAAA CTGCCTCACT GTTTGAGCAG AAGTCCATGA AAGGCTGGTG GCCGTGCTAC
    GCAGATAAAG ATGGCTCCCG CGTGATGGCT GGGAAAGTGG AGATGACGTT GGAAATCCTC
    AATGAGAAGG AGGCAGACGA GAGGCCAGCC GGGAAGGGAC GGGATGAACC CAACATGAAC
    CCCAAGCTGG ACCCACCAAA CCGACCAGAA ACCTCCTTCC TCTGGTTCAC CAACCCATGT
    AAGACCATGA AGTTCATTGT GTGGCGCCGA TTTAAGTGGG TGATCATTGG CCTGCTGATC
    CTACTGATCC TGCTGCTCTT TGTGGCCGTG CTCCTGTACT CCTTACCGAA TTATTTGTCA
    ATGAAGATTG TGAGGCCAAA TGCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005637601.1
    CDS120..6266
    Translation

    Target ORF information:

    RefSeq Version XM_005637544.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris myoferlin (MYOF), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005637544.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGCTGCGGG TGATCGTGGA GTCCGCCAGC AATATCCCGA AAACGAAATT TGGGAAACCG 
    GATCCTATCG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGACAAC
    GAACTCAATC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGAGGGGTAT ACCACTGGAC
    TTTTCATCTT CCCTTACAAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAATTA
    ATTGGCACGG CCACTGTAGC CCTGAAGGAC CTGACTGGTG ACCAGAGCAG ATCACTGCCG
    TACAAACTCA TCTCCCTGCT CAGCGAAAAG GGGCAAGATA CTGGGGCCAC CATTGACTTG
    GTGATTGGCT ATGATCCACC TTCTGCTCCA CATCCAAATG ACCCCAGTGG GACCAGTGTG
    TCAGTCACAG AAGGAGAAGA AGAGGAAGAT GGAGGTGATG AAGACAGGTT GGATGGCACA
    GTCACGGGCC CTGGGCCCAG GGGGTCAGGT GGGATCGTGT CGGAGGCTCA CCTTGCCCGG
    AGGCTCACGA AAGCAAAGAA GAACCGGCGG ATGCTGTCCA ACAAGCCTCA GGACTTCCAG
    ATCCGCGTCC GAGTGATTGA GGGCCGTCAG TTAAGTGGCA ACAACATCAA GCCTGTGGTC
    AAGGTCCATG TCTGTGGCCA GACACACAGA ACAAGGATCA AGAGAGGGAA CAACCCATTT
    TATGATGAGT TATTTTTCTA CAATGTCCAC ATAACCCCTT CTGAACTGAT GGATGAGATC
    ATCAGCATTC GGGTTTATAA TTCCCATTCT TTGCGGGCAG ATTGTCTGAT GGGGGAATTT
    AAGATTGATG TTGGATTCAT TTATGATGAA CCGGGTCACG CTGTCATGAG AAAATGGCTT
    CTTCTCAATG ACCCTGAAGA TGCCAGCTCA GGTGCTAAAG GTTACATGAA AGTCAGCATG
    TTCATCCTGG GAACTGGAGA TGAGCCTCCT GCTGAGAAGC GAGATCGTGA TAATGACAGT
    GATGACGTGG AGAGTAATTT GTTACTCCCT GCTGGCATCG CCCTCCGGTG GGTGACCTTC
    TTGCTTAAAA TCTACCGAGC AGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA
    GTAAAGGAAA TATTTGGAGG CAATGCAGAT AAAAAAAACC TAGTGGATCC TTTTGTAGAA
    GTTTCCTTTG CTGGAAAAAA GGTTTGCACA AATATAATTG AGAAAAATGC AAATCCTGAG
    TGGAACCAAG TCGTTAATCT TCAGATAAAG TTTCCTTCAA TGTGTGAAAA AATAAAACTA
    ACAATATATG ACTGGGACCG TCTTACTAAA AATGATGTTG TTGGAACAAC ATATCTACAC
    CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGCAA ATACAGGAGA AACAGAGGTG
    GGCTTTGTTC CAACATTTGG GCCTTGTTAT CTGAACCTTT ACGGAAGCCC TAGAGAGTAC
    ACAGGATTCC CAGACCCCTA TGATGAGCTA AATACTGGAA AGGGGGAAGG AGTTGCCTAC
    AGAGGCAGAA TCTTGGTTGA ATTAAGCACT TTTCTTGAAA AGACACCACC AGACAAAAAG
    CTTGAGCCTA TTTCAAATGA TGACCTGCTG GTTGTGGAGA AATACCAACG AAGGCGGAAG
    TATAGCCTGT CTGCCGTGTT CCATTCGGCC ACCATGTTGC AAGATGTTGG CGAGGCCATT
    CAGTTTGAAG TCAGCATTGG GAACTATGGC AACAAGTTTG ACACGACTTG TAAGCCTTTG
    GCATCAACCA CTCAATACAG CCGTGCCGTA TTTGATGGGA ACTACTATTA TTACTTGCCT
    TGGGCCCACA CAAAGCCGGT TGTTACCCTG ACTTCATACT GGGAGGACAT CAGTCATCGC
    CTGGATGCAG TGAACACTCT CCTAACCATG GCAGAACGGC TGCAATTGAA CTTAGAGGCT
    CTGAAATCAG GGATGCAAGG TAAAGTTCCT GCAAACCAGT TGGCTGAAAT ATGGTTGAAG
    CTGATCGATG AAGTTATAGA AGATACAAGG TACACATTGC CTGTCACGGA AGGAAAAGCT
    AATGTCACAA TTCTTGACAC TCAGATCCGA AAGTTGCGGT CCCGATCTCT CTCCCAGATT
    CATGAGGCAG CTGTGAGGAT GAGGTCTGAG GCCACAGAGG TGAAATCCAC ACTGGCAGAA
    ATTGAGGACT GGCTTGAAAA ATTACTGCAA CTGACTGAAG AGCCACAGAA CAGTATGCCT
    GACATTATCA TCTGGATGAT CCGGGGGGAG AAAAGACTGG CCTATGCACG CATCCCTGCC
    CATCAGGTTC TATACTCCAC CAGTGGTGAG ACTGCATCTG GGAAATACTG CGGGAAAACC
    CAAACCATCT TGCTGAAGTA TCCGCAGGAA AAAACCCACG GACCAAAGGT GCCCGCAGAG
    CTGCGAGTCA ACATCTGGCT GGGCTTAAGT GCTGTTGAGA AGAAATTCAA TAGCTTCGCA
    GAAGGAACAT TCACCGTCTT TGCTGAAATG TATGAAAATC AAGCTCTTAT GTTTGGAAAA
    TGGGGCACTT CTGGATTAGT GGGTCGTCAT AAATTTTCTG ATGTCACAGG AAAAATAAAA
    CTCAAGAGGG AATTCTTTTT GCCCCCGAAA GGCTGGGAAT GGGAAGGAGA GTGGATAGTT
    GATCCTGAAA GAAGCTTGCT GACTGAAGCA GACGCAGGGC ACACGGAGTT CACGGACGAA
    GTGTACCAGA ATGAGAGCCG CTACCCTGGG GGTGACTGGA AGCCAGCTGA GGACACCTAT
    ACCGATGCGA ATGGTGATAA AGCGGCATCA CCTAGCGAGC TGACATGCCC TCCAGGATGG
    GAATGGGAAG ATGACGCATG GGTATATGAT ATAAATCGTG CAGTGGATGA GAAAGGCTGG
    GAGTATGGAA TTACCATTCC TCCTGATACT AAACCCAAAT CCTGGGTTGC AGCAGAGAAA
    ATGTATCATA CTCATAGACG ACGAAGGCTA GTCCGAAAAC GCAAGAAAGA TTTAACACAA
    AGCACTGCAA GCACTGCAAG GGCCATGGAA GAATTGGAAG ACCGGGAGGG CTGGGAATAT
    GCTTCTTTAA TTGGCTGGAA ATTTCACTGG AAACAGCGTA GCTCAGATAC CTTCCGCCGT
    AGACGCTGGA GAAGGAAAAT GGCTCCTTCG GAAACGCACG GTGCGGCCGC CATCTTTAAA
    CTGGAGGGCG CCCTCGGTGC AGACATGACC GAGGATGGAG ACGAGAAGAG TGTGGACAAG
    CAGAAGTATA GTGCCACCAC TGTATTTGGG GCAAACACTC CCATTGTTTC CTGTAACTTT
    GACAGAGTCT ACATTTACCA TCTGCGCTGC TATATCTATC AAGCCAGAAA CCTCATGGCT
    CTAGACAAGG ATAGTTTTTC AGATCCATAT GCACATGTCT CCTTCCTCCA TCGAAGCAAA
    ACCACTGAGA TCATCCACTC GACTCTGAAT CCCACATGGG ACCAGACAAT TATATTTGAT
    GAAATCGAAA TCTATGGGGA ACCCCAGACA GTTCTACAGA ACCCACCCAA AGTTATCATC
    GAACTTTTTG ACAATGACCA AGTGGGCAAA GATGAATTTT TAGGACGAAG CATGTGCTCT
    CCTCTGGTAA AACTAAACTC AGAAACGGAT GTCACACCCA AACTTCTCTG GCACCCTGTA
    ATGAATGGAG ACAAAGCCTG TGGGGATGTC CTTGTAACTG CAGAGCTGAT TCTAAGGCAC
    AAGGATGGTT CCAATCTTCC CATCCTTCCC TCACAAAGGG CACCAAATCT ATACATGGTC
    CCACAAGGGA TCAGGCCTGT GGTCCAACTC ACTGCTATTG AGATTCTAGC TTGGGGCTTA
    AGAAATATGA AAAACTACCA GATGGCTTCT ATCACCTCCC CCAGCCTCAT TGTGGAGTGT
    GGAGGGGAAA GGGTAGAATC AGTGGTGATC AAAAACCTGA AGAAGACACC CAACTTCCCA
    AGTTCTGTTC TCTTCATGAA AGTGTTCTTG CCCAAGGAGG AACTGTACAT GCCTCCGCTG
    GTGATAAAGG TCATCGACCA CAGGCAGTTC GGGCGGAAGC CCGTTGTTGG GCAGTGCACC
    ATCGATCGTC TGGACCGCTT TCGTTGTGAC CCTTATGCGG GAAAGGAGGA TCTTGTGCCC
    CAGCTAAAAG CCTCCCTTCT ATCTACCCCA CCCTGCCGGG ATGTCATTAT CGAAATAGAA
    GACACCAAAC CATTACTGGC TTCGAAGCTG ACAGAAAAGG AGGAAGAAAT CGTTGACTGG
    TGGAGTAAAT TTTATGCTTC CACAGGAGAA CATGAAAAAT GTGGACAATA TGTTCAGAAA
    GGATATTCGA AACTCAAGAT ATATGATTGT GAACTAGAGG ATGTAACAGA ATTTGAAGGC
    CTGACAGACT TCTCAGATAC TTTTAAGCTG TATCGAGGCA AATCAGATGA AAATGAAGAT
    CCCTCAGTGG TAGGAGAGTT TAAGGGCTCC TTTAGAATCT ACTCTCTGCC GGATGACCCC
    AGCGTGCCGG CCCCTCCCAG GCAGTTTCGG GAATTACCTG ACAGCGTCCC ACAGGAATGC
    ACGGTTAGGA TTTACATTGT TCGAGGCTTA GAGCTCCAGC CCCAGGACAA CAATGGCCTG
    TGTGACCCTT ATATAAAAAT AACACTGGGC AAAAAAGTAA TAGAAGATCG AGACCACTAT
    ATCCCCAACA CCCTCAACCC AGTTTTCGGC AGAATGTATG AACTGAGCTG CTACTTACCT
    CAAGAAAAAG ATCTGAAAAT TTCTGTCTAT GACTACGACA CCTTTACCCG TGATGAAAAG
    GTGGGAGAAA CAATTATTGA TCTGGAAAAC CGATTCCTTT CCCGGTTTGG GTCTCACTGC
    GGCATACCTG AGCAGTACTG TGTTTCTGGA GTTAACACCT GGCGAGATCA ACTGAGACCA
    ACTCAGTTGC TTCAAAATGT AGCCAGGTTC AAAGGCTTCC CACCACCCAT CCTTTCTGAA
    GATGGAAATA GAATCAGATA TGGAGGGCGA GACTACAGTT TGGATGAATT TGAAGCCAAC
    AAAATCCTGC ACCAGCACCT CGGGGCCCCC GATGAGCGTC TGGCCCTTCA CATCCTCAGG
    ACTCAGGGGC TGGTCCCCGA GCATGTGGAA ACGAGGACTT TGCACAGCAC CTTCCAGCCC
    AACATTTCCC AGGGGAAACT TCAGATGTGG GTGGATGTTT TCCCTAAGAG TTTGGGGCCA
    CCAGGCCCTC CTTTCAACAT CACCCCCAGG AAAGCTAAAA AATACTACCT GCGTGTGATC
    ATCTGGAATA CCAAGGACGT TCTCTTGGAT GAGAAGAGCA TCACAGGAGA GGAAATGAGT
    GACATCTATG TCAAAGGCTG GATTCCTGGC AATGAAGAAA ACAAACAGAA AACAGATGTC
    CATTACAGGT CCTTGGACGG GGAAGGGAAT TTTAACTGGA GATTTGTTTT CCCATTTGAC
    TACCTCCCAG CTGAACAACT CTGTATCGTT GCTAAAAAAG AGCATTTCTG GAGTATTGAC
    CAAACTGAAT TTCGGGTCCC ACCCAGACTG ATCATTCAGA TATGGGATAA TGACAAGTTT
    TCTCTGGATG ACTATTTGGG TTTCCTGGAA CTTGATTTGC ATCACACCAT CATTCCAGCA
    AAATCATCAG AGAAATGCAG TTTGGATATG ATTCCAGACC TCAAAGCCAT GAACCCCCTT
    AAAGCAAAAA CTGCCTCACT GTTTGAGCAG AAGTCCATGA AAGGCTGGTG GCCGTGCTAC
    GCAGATAAAG ATGGCTCCCG CGTGATGGCT GGGAAAGTGG AGATGACGTT GGAAATCCTC
    AATGAGAAGG AGGCAGACGA GAGGCCAGCC GGGAAGGGAC GGGATGAACC CAACATGAAC
    CCCAAGCTGG ACCCACCAAA CCGACCAGAA ACCTCCTTCC TCTGGTTCAC CAACCCATGT
    AAGACCATGA AGTTCATTGT GTGGCGCCGA TTTAAGTGGG TGATCATTGG CCTGCTGATC
    CTACTGATCC TGCTGCTCTT TGTGGCCGTG CTCCTGTACT CCTTACCGAA TTATTTGTCA
    ATGAAGATTG TGAGGCCAAA TGCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27787
    Clone ID Related Accession (Same CDS sequence) XM_005637544.3 , XM_005637544.2
    Accession Version XM_005637544.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6147bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product myoferlin isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005637544.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGCTGCGGG TGATCGTGGA GTCCGCCAGC AATATCCCGA AAACGAAATT TGGGAAACCG 
    GATCCTATCG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGACAAC
    GAACTCAATC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGAGGGGTAT ACCACTGGAC
    TTTTCATCTT CCCTTACAAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAATTA
    ATTGGCACGG CCACTGTAGC CCTGAAGGAC CTGACTGGTG ACCAGAGCAG ATCACTGCCG
    TACAAACTCA TCTCCCTGCT CAGCGAAAAG GGGCAAGATA CTGGGGCCAC CATTGACTTG
    GTGATTGGCT ATGATCCACC TTCTGCTCCA CATCCAAATG ACCCCAGTGG GACCAGTGTG
    TCAGTCACAG AAGGAGAAGA AGAGGAAGAT GGAGGTGATG AAGACAGGTT GGATGGCACA
    GTCACGGGCC CTGGGCCCAG GGGGTCAGGT GGGATCGTGT CGGAGGCTCA CCTTGCCCGG
    AGGCTCACGA AAGCAAAGAA GAACCGGCGG ATGCTGTCCA ACAAGCCTCA GGACTTCCAG
    ATCCGCGTCC GAGTGATTGA GGGCCGTCAG TTAAGTGGCA ACAACATCAA GCCTGTGGTC
    AAGGTCCATG TCTGTGGCCA GACACACAGA ACAAGGATCA AGAGAGGGAA CAACCCATTT
    TATGATGAGT TATTTTTCTA CAATGTCCAC ATAACCCCTT CTGAACTGAT GGATGAGATC
    ATCAGCATTC GGGTTTATAA TTCCCATTCT TTGCGGGCAG ATTGTCTGAT GGGGGAATTT
    AAGATTGATG TTGGATTCAT TTATGATGAA CCGGGTCACG CTGTCATGAG AAAATGGCTT
    CTTCTCAATG ACCCTGAAGA TGCCAGCTCA GGTGCTAAAG GTTACATGAA AGTCAGCATG
    TTCATCCTGG GAACTGGAGA TGAGCCTCCT GCTGAGAAGC GAGATCGTGA TAATGACAGT
    GATGACGTGG AGAGTAATTT GTTACTCCCT GCTGGCATCG CCCTCCGGTG GGTGACCTTC
    TTGCTTAAAA TCTACCGAGC AGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA
    GTAAAGGAAA TATTTGGAGG CAATGCAGAT AAAAAAAACC TAGTGGATCC TTTTGTAGAA
    GTTTCCTTTG CTGGAAAAAA GGTTTGCACA AATATAATTG AGAAAAATGC AAATCCTGAG
    TGGAACCAAG TCGTTAATCT TCAGATAAAG TTTCCTTCAA TGTGTGAAAA AATAAAACTA
    ACAATATATG ACTGGGACCG TCTTACTAAA AATGATGTTG TTGGAACAAC ATATCTACAC
    CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGCAA ATACAGGAGA AACAGAGGTG
    GGCTTTGTTC CAACATTTGG GCCTTGTTAT CTGAACCTTT ACGGAAGCCC TAGAGAGTAC
    ACAGGATTCC CAGACCCCTA TGATGAGCTA AATACTGGAA AGGGGGAAGG AGTTGCCTAC
    AGAGGCAGAA TCTTGGTTGA ATTAAGCACT TTTCTTGAAA AGACACCACC AGACAAAAAG
    CTTGAGCCTA TTTCAAATGA TGACCTGCTG GTTGTGGAGA AATACCAACG AAGGCGGAAG
    TATAGCCTGT CTGCCGTGTT CCATTCGGCC ACCATGTTGC AAGATGTTGG CGAGGCCATT
    CAGTTTGAAG TCAGCATTGG GAACTATGGC AACAAGTTTG ACACGACTTG TAAGCCTTTG
    GCATCAACCA CTCAATACAG CCGTGCCGTA TTTGATGGGA ACTACTATTA TTACTTGCCT
    TGGGCCCACA CAAAGCCGGT TGTTACCCTG ACTTCATACT GGGAGGACAT CAGTCATCGC
    CTGGATGCAG TGAACACTCT CCTAACCATG GCAGAACGGC TGCAATTGAA CTTAGAGGCT
    CTGAAATCAG GGATGCAAGG TAAAGTTCCT GCAAACCAGT TGGCTGAAAT ATGGTTGAAG
    CTGATCGATG AAGTTATAGA AGATACAAGG TACACATTGC CTGTCACGGA AGGAAAAGCT
    AATGTCACAA TTCTTGACAC TCAGATCCGA AAGTTGCGGT CCCGATCTCT CTCCCAGATT
    CATGAGGCAG CTGTGAGGAT GAGGTCTGAG GCCACAGAGG TGAAATCCAC ACTGGCAGAA
    ATTGAGGACT GGCTTGAAAA ATTACTGCAA CTGACTGAAG AGCCACAGAA CAGTATGCCT
    GACATTATCA TCTGGATGAT CCGGGGGGAG AAAAGACTGG CCTATGCACG CATCCCTGCC
    CATCAGGTTC TATACTCCAC CAGTGGTGAG ACTGCATCTG GGAAATACTG CGGGAAAACC
    CAAACCATCT TGCTGAAGTA TCCGCAGGAA AAAACCCACG GACCAAAGGT GCCCGCAGAG
    CTGCGAGTCA ACATCTGGCT GGGCTTAAGT GCTGTTGAGA AGAAATTCAA TAGCTTCGCA
    GAAGGAACAT TCACCGTCTT TGCTGAAATG TATGAAAATC AAGCTCTTAT GTTTGGAAAA
    TGGGGCACTT CTGGATTAGT GGGTCGTCAT AAATTTTCTG ATGTCACAGG AAAAATAAAA
    CTCAAGAGGG AATTCTTTTT GCCCCCGAAA GGCTGGGAAT GGGAAGGAGA GTGGATAGTT
    GATCCTGAAA GAAGCTTGCT GACTGAAGCA GACGCAGGGC ACACGGAGTT CACGGACGAA
    GTGTACCAGA ATGAGAGCCG CTACCCTGGG GGTGACTGGA AGCCAGCTGA GGACACCTAT
    ACCGATGCGA ATGGTGATAA AGCGGCATCA CCTAGCGAGC TGACATGCCC TCCAGGATGG
    GAATGGGAAG ATGACGCATG GGTATATGAT ATAAATCGTG CAGTGGATGA GAAAGGCTGG
    GAGTATGGAA TTACCATTCC TCCTGATACT AAACCCAAAT CCTGGGTTGC AGCAGAGAAA
    ATGTATCATA CTCATAGACG ACGAAGGCTA GTCCGAAAAC GCAAGAAAGA TTTAACACAA
    AGCACTGCAA GCACTGCAAG GGCCATGGAA GAATTGGAAG ACCGGGAGGG CTGGGAATAT
    GCTTCTTTAA TTGGCTGGAA ATTTCACTGG AAACAGCGTA GCTCAGATAC CTTCCGCCGT
    AGACGCTGGA GAAGGAAAAT GGCTCCTTCG GAAACGCACG GTGCGGCCGC CATCTTTAAA
    CTGGAGGGCG CCCTCGGTGC AGACATGACC GAGGATGGAG ACGAGAAGAG TGTGGACAAG
    CAGAAGTATA GTGCCACCAC TGTATTTGGG GCAAACACTC CCATTGTTTC CTGTAACTTT
    GACAGAGTCT ACATTTACCA TCTGCGCTGC TATATCTATC AAGCCAGAAA CCTCATGGCT
    CTAGACAAGG ATAGTTTTTC AGATCCATAT GCACATGTCT CCTTCCTCCA TCGAAGCAAA
    ACCACTGAGA TCATCCACTC GACTCTGAAT CCCACATGGG ACCAGACAAT TATATTTGAT
    GAAATCGAAA TCTATGGGGA ACCCCAGACA GTTCTACAGA ACCCACCCAA AGTTATCATC
    GAACTTTTTG ACAATGACCA AGTGGGCAAA GATGAATTTT TAGGACGAAG CATGTGCTCT
    CCTCTGGTAA AACTAAACTC AGAAACGGAT GTCACACCCA AACTTCTCTG GCACCCTGTA
    ATGAATGGAG ACAAAGCCTG TGGGGATGTC CTTGTAACTG CAGAGCTGAT TCTAAGGCAC
    AAGGATGGTT CCAATCTTCC CATCCTTCCC TCACAAAGGG CACCAAATCT ATACATGGTC
    CCACAAGGGA TCAGGCCTGT GGTCCAACTC ACTGCTATTG AGATTCTAGC TTGGGGCTTA
    AGAAATATGA AAAACTACCA GATGGCTTCT ATCACCTCCC CCAGCCTCAT TGTGGAGTGT
    GGAGGGGAAA GGGTAGAATC AGTGGTGATC AAAAACCTGA AGAAGACACC CAACTTCCCA
    AGTTCTGTTC TCTTCATGAA AGTGTTCTTG CCCAAGGAGG AACTGTACAT GCCTCCGCTG
    GTGATAAAGG TCATCGACCA CAGGCAGTTC GGGCGGAAGC CCGTTGTTGG GCAGTGCACC
    ATCGATCGTC TGGACCGCTT TCGTTGTGAC CCTTATGCGG GAAAGGAGGA TCTTGTGCCC
    CAGCTAAAAG CCTCCCTTCT ATCTACCCCA CCCTGCCGGG ATGTCATTAT CGAAATAGAA
    GACACCAAAC CATTACTGGC TTCGAAGCTG ACAGAAAAGG AGGAAGAAAT CGTTGACTGG
    TGGAGTAAAT TTTATGCTTC CACAGGAGAA CATGAAAAAT GTGGACAATA TGTTCAGAAA
    GGATATTCGA AACTCAAGAT ATATGATTGT GAACTAGAGG ATGTAACAGA ATTTGAAGGC
    CTGACAGACT TCTCAGATAC TTTTAAGCTG TATCGAGGCA AATCAGATGA AAATGAAGAT
    CCCTCAGTGG TAGGAGAGTT TAAGGGCTCC TTTAGAATCT ACTCTCTGCC GGATGACCCC
    AGCGTGCCGG CCCCTCCCAG GCAGTTTCGG GAATTACCTG ACAGCGTCCC ACAGGAATGC
    ACGGTTAGGA TTTACATTGT TCGAGGCTTA GAGCTCCAGC CCCAGGACAA CAATGGCCTG
    TGTGACCCTT ATATAAAAAT AACACTGGGC AAAAAAGTAA TAGAAGATCG AGACCACTAT
    ATCCCCAACA CCCTCAACCC AGTTTTCGGC AGAATGTATG AACTGAGCTG CTACTTACCT
    CAAGAAAAAG ATCTGAAAAT TTCTGTCTAT GACTACGACA CCTTTACCCG TGATGAAAAG
    GTGGGAGAAA CAATTATTGA TCTGGAAAAC CGATTCCTTT CCCGGTTTGG GTCTCACTGC
    GGCATACCTG AGCAGTACTG TGTTTCTGGA GTTAACACCT GGCGAGATCA ACTGAGACCA
    ACTCAGTTGC TTCAAAATGT AGCCAGGTTC AAAGGCTTCC CACCACCCAT CCTTTCTGAA
    GATGGAAATA GAATCAGATA TGGAGGGCGA GACTACAGTT TGGATGAATT TGAAGCCAAC
    AAAATCCTGC ACCAGCACCT CGGGGCCCCC GATGAGCGTC TGGCCCTTCA CATCCTCAGG
    ACTCAGGGGC TGGTCCCCGA GCATGTGGAA ACGAGGACTT TGCACAGCAC CTTCCAGCCC
    AACATTTCCC AGGGGAAACT TCAGATGTGG GTGGATGTTT TCCCTAAGAG TTTGGGGCCA
    CCAGGCCCTC CTTTCAACAT CACCCCCAGG AAAGCTAAAA AATACTACCT GCGTGTGATC
    ATCTGGAATA CCAAGGACGT TCTCTTGGAT GAGAAGAGCA TCACAGGAGA GGAAATGAGT
    GACATCTATG TCAAAGGCTG GATTCCTGGC AATGAAGAAA ACAAACAGAA AACAGATGTC
    CATTACAGGT CCTTGGACGG GGAAGGGAAT TTTAACTGGA GATTTGTTTT CCCATTTGAC
    TACCTCCCAG CTGAACAACT CTGTATCGTT GCTAAAAAAG AGCATTTCTG GAGTATTGAC
    CAAACTGAAT TTCGGGTCCC ACCCAGACTG ATCATTCAGA TATGGGATAA TGACAAGTTT
    TCTCTGGATG ACTATTTGGG TTTCCTGGAA CTTGATTTGC ATCACACCAT CATTCCAGCA
    AAATCATCAG AGAAATGCAG TTTGGATATG ATTCCAGACC TCAAAGCCAT GAACCCCCTT
    AAAGCAAAAA CTGCCTCACT GTTTGAGCAG AAGTCCATGA AAGGCTGGTG GCCGTGCTAC
    GCAGATAAAG ATGGCTCCCG CGTGATGGCT GGGAAAGTGG AGATGACGTT GGAAATCCTC
    AATGAGAAGG AGGCAGACGA GAGGCCAGCC GGGAAGGGAC GGGATGAACC CAACATGAAC
    CCCAAGCTGG ACCCACCAAA CCGACCAGAA ACCTCCTTCC TCTGGTTCAC CAACCCATGT
    AAGACCATGA AGTTCATTGT GTGGCGCCGA TTTAAGTGGG TGATCATTGG CCTGCTGATC
    CTACTGATCC TGCTGCTCTT TGTGGCCGTG CTCCTGTACT CCTTACCGAA TTATTTGTCA
    ATGAAGATTG TGAGGCCAAA TGCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005637601.1
    CDS568..6714
    Translation

    Target ORF information:

    RefSeq Version XM_005637544.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris myoferlin (MYOF), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005637544.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGCTGCGGG TGATCGTGGA GTCCGCCAGC AATATCCCGA AAACGAAATT TGGGAAACCG 
    GATCCTATCG TTTCTGTCAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTTGACAAC
    GAACTCAATC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGAGGGGTAT ACCACTGGAC
    TTTTCATCTT CCCTTACAAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAATTA
    ATTGGCACGG CCACTGTAGC CCTGAAGGAC CTGACTGGTG ACCAGAGCAG ATCACTGCCG
    TACAAACTCA TCTCCCTGCT CAGCGAAAAG GGGCAAGATA CTGGGGCCAC CATTGACTTG
    GTGATTGGCT ATGATCCACC TTCTGCTCCA CATCCAAATG ACCCCAGTGG GACCAGTGTG
    TCAGTCACAG AAGGAGAAGA AGAGGAAGAT GGAGGTGATG AAGACAGGTT GGATGGCACA
    GTCACGGGCC CTGGGCCCAG GGGGTCAGGT GGGATCGTGT CGGAGGCTCA CCTTGCCCGG
    AGGCTCACGA AAGCAAAGAA GAACCGGCGG ATGCTGTCCA ACAAGCCTCA GGACTTCCAG
    ATCCGCGTCC GAGTGATTGA GGGCCGTCAG TTAAGTGGCA ACAACATCAA GCCTGTGGTC
    AAGGTCCATG TCTGTGGCCA GACACACAGA ACAAGGATCA AGAGAGGGAA CAACCCATTT
    TATGATGAGT TATTTTTCTA CAATGTCCAC ATAACCCCTT CTGAACTGAT GGATGAGATC
    ATCAGCATTC GGGTTTATAA TTCCCATTCT TTGCGGGCAG ATTGTCTGAT GGGGGAATTT
    AAGATTGATG TTGGATTCAT TTATGATGAA CCGGGTCACG CTGTCATGAG AAAATGGCTT
    CTTCTCAATG ACCCTGAAGA TGCCAGCTCA GGTGCTAAAG GTTACATGAA AGTCAGCATG
    TTCATCCTGG GAACTGGAGA TGAGCCTCCT GCTGAGAAGC GAGATCGTGA TAATGACAGT
    GATGACGTGG AGAGTAATTT GTTACTCCCT GCTGGCATCG CCCTCCGGTG GGTGACCTTC
    TTGCTTAAAA TCTACCGAGC AGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA
    GTAAAGGAAA TATTTGGAGG CAATGCAGAT AAAAAAAACC TAGTGGATCC TTTTGTAGAA
    GTTTCCTTTG CTGGAAAAAA GGTTTGCACA AATATAATTG AGAAAAATGC AAATCCTGAG
    TGGAACCAAG TCGTTAATCT TCAGATAAAG TTTCCTTCAA TGTGTGAAAA AATAAAACTA
    ACAATATATG ACTGGGACCG TCTTACTAAA AATGATGTTG TTGGAACAAC ATATCTACAC
    CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGCAA ATACAGGAGA AACAGAGGTG
    GGCTTTGTTC CAACATTTGG GCCTTGTTAT CTGAACCTTT ACGGAAGCCC TAGAGAGTAC
    ACAGGATTCC CAGACCCCTA TGATGAGCTA AATACTGGAA AGGGGGAAGG AGTTGCCTAC
    AGAGGCAGAA TCTTGGTTGA ATTAAGCACT TTTCTTGAAA AGACACCACC AGACAAAAAG
    CTTGAGCCTA TTTCAAATGA TGACCTGCTG GTTGTGGAGA AATACCAACG AAGGCGGAAG
    TATAGCCTGT CTGCCGTGTT CCATTCGGCC ACCATGTTGC AAGATGTTGG CGAGGCCATT
    CAGTTTGAAG TCAGCATTGG GAACTATGGC AACAAGTTTG ACACGACTTG TAAGCCTTTG
    GCATCAACCA CTCAATACAG CCGTGCCGTA TTTGATGGGA ACTACTATTA TTACTTGCCT
    TGGGCCCACA CAAAGCCGGT TGTTACCCTG ACTTCATACT GGGAGGACAT CAGTCATCGC
    CTGGATGCAG TGAACACTCT CCTAACCATG GCAGAACGGC TGCAATTGAA CTTAGAGGCT
    CTGAAATCAG GGATGCAAGG TAAAGTTCCT GCAAACCAGT TGGCTGAAAT ATGGTTGAAG
    CTGATCGATG AAGTTATAGA AGATACAAGG TACACATTGC CTGTCACGGA AGGAAAAGCT
    AATGTCACAA TTCTTGACAC TCAGATCCGA AAGTTGCGGT CCCGATCTCT CTCCCAGATT
    CATGAGGCAG CTGTGAGGAT GAGGTCTGAG GCCACAGAGG TGAAATCCAC ACTGGCAGAA
    ATTGAGGACT GGCTTGAAAA ATTACTGCAA CTGACTGAAG AGCCACAGAA CAGTATGCCT
    GACATTATCA TCTGGATGAT CCGGGGGGAG AAAAGACTGG CCTATGCACG CATCCCTGCC
    CATCAGGTTC TATACTCCAC CAGTGGTGAG ACTGCATCTG GGAAATACTG CGGGAAAACC
    CAAACCATCT TGCTGAAGTA TCCGCAGGAA AAAACCCACG GACCAAAGGT GCCCGCAGAG
    CTGCGAGTCA ACATCTGGCT GGGCTTAAGT GCTGTTGAGA AGAAATTCAA TAGCTTCGCA
    GAAGGAACAT TCACCGTCTT TGCTGAAATG TATGAAAATC AAGCTCTTAT GTTTGGAAAA
    TGGGGCACTT CTGGATTAGT GGGTCGTCAT AAATTTTCTG ATGTCACAGG AAAAATAAAA
    CTCAAGAGGG AATTCTTTTT GCCCCCGAAA GGCTGGGAAT GGGAAGGAGA GTGGATAGTT
    GATCCTGAAA GAAGCTTGCT GACTGAAGCA GACGCAGGGC ACACGGAGTT CACGGACGAA
    GTGTACCAGA ATGAGAGCCG CTACCCTGGG GGTGACTGGA AGCCAGCTGA GGACACCTAT
    ACCGATGCGA ATGGTGATAA AGCGGCATCA CCTAGCGAGC TGACATGCCC TCCAGGATGG
    GAATGGGAAG ATGACGCATG GGTATATGAT ATAAATCGTG CAGTGGATGA GAAAGGCTGG
    GAGTATGGAA TTACCATTCC TCCTGATACT AAACCCAAAT CCTGGGTTGC AGCAGAGAAA
    ATGTATCATA CTCATAGACG ACGAAGGCTA GTCCGAAAAC GCAAGAAAGA TTTAACACAA
    AGCACTGCAA GCACTGCAAG GGCCATGGAA GAATTGGAAG ACCGGGAGGG CTGGGAATAT
    GCTTCTTTAA TTGGCTGGAA ATTTCACTGG AAACAGCGTA GCTCAGATAC CTTCCGCCGT
    AGACGCTGGA GAAGGAAAAT GGCTCCTTCG GAAACGCACG GTGCGGCCGC CATCTTTAAA
    CTGGAGGGCG CCCTCGGTGC AGACATGACC GAGGATGGAG ACGAGAAGAG TGTGGACAAG
    CAGAAGTATA GTGCCACCAC TGTATTTGGG GCAAACACTC CCATTGTTTC CTGTAACTTT
    GACAGAGTCT ACATTTACCA TCTGCGCTGC TATATCTATC AAGCCAGAAA CCTCATGGCT
    CTAGACAAGG ATAGTTTTTC AGATCCATAT GCACATGTCT CCTTCCTCCA TCGAAGCAAA
    ACCACTGAGA TCATCCACTC GACTCTGAAT CCCACATGGG ACCAGACAAT TATATTTGAT
    GAAATCGAAA TCTATGGGGA ACCCCAGACA GTTCTACAGA ACCCACCCAA AGTTATCATC
    GAACTTTTTG ACAATGACCA AGTGGGCAAA GATGAATTTT TAGGACGAAG CATGTGCTCT
    CCTCTGGTAA AACTAAACTC AGAAACGGAT GTCACACCCA AACTTCTCTG GCACCCTGTA
    ATGAATGGAG ACAAAGCCTG TGGGGATGTC CTTGTAACTG CAGAGCTGAT TCTAAGGCAC
    AAGGATGGTT CCAATCTTCC CATCCTTCCC TCACAAAGGG CACCAAATCT ATACATGGTC
    CCACAAGGGA TCAGGCCTGT GGTCCAACTC ACTGCTATTG AGATTCTAGC TTGGGGCTTA
    AGAAATATGA AAAACTACCA GATGGCTTCT ATCACCTCCC CCAGCCTCAT TGTGGAGTGT
    GGAGGGGAAA GGGTAGAATC AGTGGTGATC AAAAACCTGA AGAAGACACC CAACTTCCCA
    AGTTCTGTTC TCTTCATGAA AGTGTTCTTG CCCAAGGAGG AACTGTACAT GCCTCCGCTG
    GTGATAAAGG TCATCGACCA CAGGCAGTTC GGGCGGAAGC CCGTTGTTGG GCAGTGCACC
    ATCGATCGTC TGGACCGCTT TCGTTGTGAC CCTTATGCGG GAAAGGAGGA TCTTGTGCCC
    CAGCTAAAAG CCTCCCTTCT ATCTACCCCA CCCTGCCGGG ATGTCATTAT CGAAATAGAA
    GACACCAAAC CATTACTGGC TTCGAAGCTG ACAGAAAAGG AGGAAGAAAT CGTTGACTGG
    TGGAGTAAAT TTTATGCTTC CACAGGAGAA CATGAAAAAT GTGGACAATA TGTTCAGAAA
    GGATATTCGA AACTCAAGAT ATATGATTGT GAACTAGAGG ATGTAACAGA ATTTGAAGGC
    CTGACAGACT TCTCAGATAC TTTTAAGCTG TATCGAGGCA AATCAGATGA AAATGAAGAT
    CCCTCAGTGG TAGGAGAGTT TAAGGGCTCC TTTAGAATCT ACTCTCTGCC GGATGACCCC
    AGCGTGCCGG CCCCTCCCAG GCAGTTTCGG GAATTACCTG ACAGCGTCCC ACAGGAATGC
    ACGGTTAGGA TTTACATTGT TCGAGGCTTA GAGCTCCAGC CCCAGGACAA CAATGGCCTG
    TGTGACCCTT ATATAAAAAT AACACTGGGC AAAAAAGTAA TAGAAGATCG AGACCACTAT
    ATCCCCAACA CCCTCAACCC AGTTTTCGGC AGAATGTATG AACTGAGCTG CTACTTACCT
    CAAGAAAAAG ATCTGAAAAT TTCTGTCTAT GACTACGACA CCTTTACCCG TGATGAAAAG
    GTGGGAGAAA CAATTATTGA TCTGGAAAAC CGATTCCTTT CCCGGTTTGG GTCTCACTGC
    GGCATACCTG AGCAGTACTG TGTTTCTGGA GTTAACACCT GGCGAGATCA ACTGAGACCA
    ACTCAGTTGC TTCAAAATGT AGCCAGGTTC AAAGGCTTCC CACCACCCAT CCTTTCTGAA
    GATGGAAATA GAATCAGATA TGGAGGGCGA GACTACAGTT TGGATGAATT TGAAGCCAAC
    AAAATCCTGC ACCAGCACCT CGGGGCCCCC GATGAGCGTC TGGCCCTTCA CATCCTCAGG
    ACTCAGGGGC TGGTCCCCGA GCATGTGGAA ACGAGGACTT TGCACAGCAC CTTCCAGCCC
    AACATTTCCC AGGGGAAACT TCAGATGTGG GTGGATGTTT TCCCTAAGAG TTTGGGGCCA
    CCAGGCCCTC CTTTCAACAT CACCCCCAGG AAAGCTAAAA AATACTACCT GCGTGTGATC
    ATCTGGAATA CCAAGGACGT TCTCTTGGAT GAGAAGAGCA TCACAGGAGA GGAAATGAGT
    GACATCTATG TCAAAGGCTG GATTCCTGGC AATGAAGAAA ACAAACAGAA AACAGATGTC
    CATTACAGGT CCTTGGACGG GGAAGGGAAT TTTAACTGGA GATTTGTTTT CCCATTTGAC
    TACCTCCCAG CTGAACAACT CTGTATCGTT GCTAAAAAAG AGCATTTCTG GAGTATTGAC
    CAAACTGAAT TTCGGGTCCC ACCCAGACTG ATCATTCAGA TATGGGATAA TGACAAGTTT
    TCTCTGGATG ACTATTTGGG TTTCCTGGAA CTTGATTTGC ATCACACCAT CATTCCAGCA
    AAATCATCAG AGAAATGCAG TTTGGATATG ATTCCAGACC TCAAAGCCAT GAACCCCCTT
    AAAGCAAAAA CTGCCTCACT GTTTGAGCAG AAGTCCATGA AAGGCTGGTG GCCGTGCTAC
    GCAGATAAAG ATGGCTCCCG CGTGATGGCT GGGAAAGTGG AGATGACGTT GGAAATCCTC
    AATGAGAAGG AGGCAGACGA GAGGCCAGCC GGGAAGGGAC GGGATGAACC CAACATGAAC
    CCCAAGCTGG ACCCACCAAA CCGACCAGAA ACCTCCTTCC TCTGGTTCAC CAACCCATGT
    AAGACCATGA AGTTCATTGT GTGGCGCCGA TTTAAGTGGG TGATCATTGG CCTGCTGATC
    CTACTGATCC TGCTGCTCTT TGTGGCCGTG CTCCTGTACT CCTTACCGAA TTATTTGTCA
    ATGAAGATTG TGAGGCCAAA TGCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.