TNRC6A gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_022419937.1	XP_022275645.1	trinucleotide repeat-containing gene 6A protein isoform X7
XM_005621403.2	XP_005621460.1	trinucleotide repeat-containing gene 6A protein isoform X5
XM_005621402.2	XP_005621459.1	trinucleotide repeat-containing gene 6A protein isoform X4
XM_005621401.3	XP_005621458.1	trinucleotide repeat-containing gene 6A protein isoform X4
XM_005621401.2	XP_005621458.1	trinucleotide repeat-containing gene 6A protein isoform X3
XM_005621399.3	XP_005621456.1	trinucleotide repeat-containing gene 6A protein isoform X1
XM_014114535.2	XP_013970010.1	trinucleotide repeat-containing gene 6A protein isoform X3
XM_005621402.3	XP_005621459.1	trinucleotide repeat-containing gene 6A protein isoform X5
XM_005621399.2	XP_005621456.1	trinucleotide repeat-containing gene 6A protein isoform X1
XM_547086.5	XP_547086.3	trinucleotide repeat-containing gene 6A protein isoform X5
XM_014114536.1	XP_013970011.1	trinucleotide repeat-containing gene 6A protein isoform X7
XM_022419936.1	XP_022275644.1	trinucleotide repeat-containing gene 6A protein isoform X2
XM_005621403.3	XP_005621460.1	trinucleotide repeat-containing gene 6A protein isoform X6
XM_022419938.1	XP_022275646.1	trinucleotide repeat-containing gene 6A protein isoform X8
XM_022419939.1	XP_022275647.1	trinucleotide repeat-containing gene 6A protein isoform X10
XM_022419940.1	XP_022275648.1	trinucleotide repeat-containing gene 6A protein isoform X11
XM_014114535.1	XP_013970010.1	trinucleotide repeat-containing gene 6A protein isoform X2
XM_005621404.2	XP_005621461.1	trinucleotide repeat-containing gene 6A protein isoform X6
XM_005621404.3	XP_005621461.1	trinucleotide repeat-containing gene 6A protein isoform X9
XM_022419941.1	XP_022275649.1	trinucleotide repeat-containing gene 6A protein isoform X12

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe07956	XM_005621399.3 Latest version!	Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe07957	XM_022419936.1 Latest version!	Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe07958	XM_005621401.3 Latest version!	Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe07959	XM_005621402.3 Latest version!	Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe07960	XM_547086.5 Latest version!	Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe07960	XM_005621403.3 Latest version!	Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe07961	XM_005621404.3 Latest version!	Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe07962	XM_022419940.1 Latest version!	Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe288916	XM_022419937.1 Latest version!	Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe288917	XM_022419938.1 Latest version!	Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe288918	XM_022419939.1 Latest version!	Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe288919	XM_022419941.1 Latest version!	Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe37067	XM_014114535.2 Latest version!	Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe37068	XM_014114536.1 Latest version!	Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OCe07958	XM_005621401.2	Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe07959	XM_005621402.2	Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe07956	XM_005621399.2	Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe07960	XM_005621403.2	Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe37067	XM_014114535.1	Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe07961	XM_005621404.2	Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

TNRC6A ( XM_005621399.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005621456.1 Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe07956
Clone ID Related Accession (Same CDS sequence)	XM_005621399.3 , XM_005621399.2
Accession Version	XM_005621399.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5952bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	trinucleotide repeat-containing gene 6A protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005621399.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACTAG TGGCAGAGCC AGGCCTAGAA GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG AGAATTGGAA GCTAAAGCTA CCAAAGACGT AGAAAGGACT 
CTTAGCAGGG ATTTAGTGCA AGAAGAAGAA CAGTTGATGG AAGAAAAGAA AAAGAAAAAA 
GACGACAAGA AAAAGAAGGA AGCTGCTCAA AAGAAGGCCA CTGAACAAAA AATCAAAGTG 
CCAGAACAGA TAAAGCCCAG TGTAAGCCAG CCTCAGCCTG CCAACTCTAA TAACGGCACT 
TCCACAGCAA CCAGCACTAA TAATAATGCC AAGCGAGCCA CAGCCAACAA TCCGCAGCAG 
CAGCAGCAGC CACAGCCGCC ACAGCAGCCA CAGCAGCCGC CGCAGCAGCC ACCGCAGCCG 
CCACAGCAGC AGCCGCAGCC AGCACAGGCC TTGCCTCGGT ATCCTCGGGA AGTACCACCA 
CGATTTCGCC ACCAGGAACA CAAACAGCTT CTAAAGAGGG GTCAGCATTT TCCTGTCATA 
GCAGCAAACC TGGGATCTGC TGTTAAAGTG TTAAGCAGCC AGTCAGAAAG CAGTGCTTTA 
ACAAATCAAC AGCCACAAAA TAACGGAGAG GTGCAGAACA GCAAAAACCA GTCAGATATA 
AACCACAGTA CTTCAGGATC CCATTATGAA AATTCACAGC GGGGACCTGT GTCTTCTCCG 
AGTGACTCTA GCACAAATTG TAAGAATGCT GTTGTAAACG ACTCCCCTGA GACAGAAGCA 
TGGCCCTCAG TCCCTGGCAG TGATCCAGAG TTGGCTTCAG AATGTATGGA TGCTGATTCT 
GCCTCCAGTT CTGAATCAGA GAGAAACATC ACTATCATGG CTTCAGGGAG CACAGGTGGT 
GAAAAAGATG GCCTTCGGAA TAGCACTGGA CTTGGTTCCC AAAACAAGTT TGTGGTTGGT 
AGCAGCAGCA ATAATGTGGG CCATGGAAGT AGTCCTGGGC CATGGGGTTT TTCCCATGGA 
GCCATAATAA GCACATGTCA GGTCTCTGTG GATGCTCCTG AAAGCAAATC AGAAAGTAGC 
AACAATAGAA TGAATGCTTG GGGCACTGTA AGTTCTTCAT CAAATGGAGG GTTAAATCCA 
AGCACTTTGA ATTCAGCTAG CAACCATGGT GCCTGGCCAG TATTAGACAA CAACGGACTT 
GCCCTCAAAG GGCCTGTAGG GAGCGGTAGT TCTGGCATCA ATATTCAGTG TAGTACCATA 
GGCCAGATGC CTAACAATCA GAGTATTAAC TCTAAAGTGG GTGGTTCTTC CACCCATGGT 
ACCTGGGGAA GCCTTCAGGA AACTTGTGAA TCTGAAGTAA GTGGTACACA GAAGGTTTCA 
TTCAGTGGTC AACCTCAGAA TATTACCACT GAGATGACTG GACCAAATAA CACTACTAAC 
TTTATGACCT CTAGTTTACC AAACTCCGGT TCAGTACAGA ATAACGAACT GCCTAGTAAT 
ACAGGGGCCT GGCGTGTGAG CACAATGAAT CATCCTCAGA TACAGGCTCC ATCGGTTATG 
AATGGCACTT CCCTTTCTCA TCTTAGCAAT GGAGAGCCAA AAAGTGGAGG CTCTTACGGT 
ACTACGTGGG GTGCCTATGG TTCTAATTAC TCTGGAGACA AATGTTCGAG CCCTAACGGC 
CAAGCTAATG GTGACACTGT GAATGCAACT CTAATGCAGC CTGGCATAAA TGGGCCTATG 
GGCACTAACT TTCAAGTGAA CACAAATAAA GGAGGAGGTG TGTGGGAGTC TGGGGCAACG 
AATTCCCAGA ATGCATCGTG GGGAAGTGGA AATGGCGCAA ATTCTGGAGG AAGTCGACGA 
GGATGGGGAA CTCCTGCACA AAACACTGGC ACTAATATAC CCAGCGTGGA GTGGAGCAAA 
CTGCCTAGCA ATCAGCATTC CAATGACAGT GCAAATGGCA ATGGTAAGAA GTTTACAAAT 
GGATGGAAAT CTACTGAGGA AGAGGATCAG GCTTCTGCCG CATCTCAGAC AACTGAGCAG 
AACAGTGTGT GGGCCAAAAC AGGAGGTACA GTGGAGAGTG AAGGTAGTAC AGAAAGCACT 
GGACGCCTCG AGGAGAAAGC CACTGGGGAA AATCAGAGTA GAGATCGGAG AAAAATTGAT 
CAGCACACAT TACTCCAAAG CATCGTAAAC AGAACTGACT TGGATCCACG TGTCCTCTCC 
AACTCTGGTT GGGGACAGAC TCCTATTAAG CAGAATACTG CCTGGGATAC GGAAACATCA 
CCTAGAGGGG AAAGAAAGAC TGACAATGGG ACAGAGGCCT GGGGAAGCTC TGCAACACAG 
ACTTTTAACT CAGGGGCATG TACAGATAAG ACTAGCCCTA CTAGTAATGA TACCTCATCT 
GTATCGGGGT GGGGAGATCC CAAACCTGCT CTGAGGTGGG GAGATTCCAA AGGCTCAAGC 
TGCCAGGGGG GATGGGAAGA TGATGCTGCT GCTACAGGAA TGGTCAAGAG CAATCAGTGG 
GGGAACAGCA AAGAAGAGAA GTCCGCATGG AATGATTCGC AAAAGAGCAA ACAGGGATGG 
GGTGATGGAC AGAAATCAAA CCAAGGGTGG TCCGTTTCTG CCAGTGATAA CTGGGGAGAA 
ACGTCAAGGA GTAACCATTG GGGTGAGGCT AATAAGAAAT CTAGCTCAGG AGGTAGTGAC 
AGTGACAGGT CTGTTTCTGG TTGGAATGAA CTTGGTAAAA CTAGTTCTTT TACTTGGGGA 
AATAACATAA ATCCAAATAA TTCGTCAGGA TGGGATGAAT CTTCTAAACC TAATCCTTCC 
CAGGGATGGG GAGATCCTCC AAAGTCTAAT CAGTCTCTAG GTTGGGGAGA TTCGTCAAAG 
CCAGTCACTT CTCCAGACTG GAACAAGCAA CAAGACATTG TCGGGTCTTG GGGAATCCCA 
CCAGCAACAG GCAAACCTCC TGGTACAGGC TGGCTGGGAG GACCTATACC AGCCCCAACA 
AAAGAAGAAG AGCCCACAGG CTGGGAGGAA CCATCCCCAG AATCCATACG TCGCAAAATG 
GAGATCGATG ACGGAACTTC AGCTTGGGGA GATCCGAGCA AATACAACTA CAAAAATGTG 
AACATGTGGA ACAAAAATGT CCCAAATGGC AGCAGCCGTT CAGACCAGCA AGCACAGGTA 
CACCAGTTGC TACCATCTGC AAGTGCCATC TCAAACAAAG AGGCAGGCAG TGGCTCTGGC 
TGGGGTGAGC CCTGGGGGGA GCCTTCTACT CCAGCCACAA CTGTGGATAA TGGTACTTCA 
GCATGGGGTA AACCCATAGA CAGTGGTCCC AGCTGGGGGG AGCCCATTGC TGCAGCATCC 
AACACATCCA CGTGGGGCTC CAGCTCTGTT GGTCCTCAAG CATTAAGCAA ATCTGGGCCA 
AAATCTATGC AAGATGGCTG GTGTGGTGAT GATATGCCAT TGCCTGGAAA TCGCCCCACT 
GGCTGGGAAG AGGAAGAGGA TGTAGAGATT GGAATGTGGA ATAGTAATTC ATCTCAAGAG 
CTTAACTCAT CTTTAAATTG GCCTCCATAT ACAAAGAAAA TGTCATCGAA GGGTCTGAGT 
GGCAAAAAAA GGAGAAGGGA AAGGGGAACC ATGAAAGGTG GAAACAAACA AGAAGAAGCA 
TGGATAAATC CATTCGTTAA ACAGTTTTCA AATATCAGTT TTTCGAGAGA CTCACCCGAA 
GAAAATGTAC AGAGCAATAA GATGGATCTT TCCGGAGGAA TGTTACAGGA CAAACGAATG 
GAGATAGATA AACATAGCCT AAATATTGGT GATTACAATC GAACGGTCGG GAAAGGCCCT 
GGTTCTCGGC CTCAGATTTC CAAAGAGTCT TCCATGGAGC GCAGCCCTTA CTTTGATAAG 
GATGGCATTG TAGCAGATGA ATCCCAAAAC ATGCAGTTTA TGTCCAGTCA AAGCATGAAG 
CTTCCCCCTT CAAATAGTGC ACTACCTAAC CAGGCCCTTG GCTCCATAGC AGGGCTGGGT 
ATGCAAAACT TGAATTCTGT TAGACAGAAT GGCAATCCCA GTATGTTTGG TGTTGGAAAC 
ACAGCAGCAC AACCCCGGGG CATGCAGCAG CCTCCAGCAC AACCTCTTAG TTCATCTCAG 
CCTAATCTTC GTGCTCAAGT GCCTCCTCCA TTACTCTCCC CTCAGGTTCC AGTTTCATTG 
CTGAAGTATG CACCAAACAA CGGTGGCCTG AATCCGCTCT TTGGCCCTCA ACAGGTAGCC 
ATGCTGAACC AGCTATCCCA ACTAAACCAG CTTTCTCAGA TCTCCCAGTT ACAGAGATTG 
TTAGCGCAGC AGCAAAGGGC GCAGAGTCAG AGAAGCGTGC CTTCTGGGAA CCGGCAGCAG 
CAAGACCAGC AGGGTCGACC TCTTAGCGTG CAGCAGCAGA TGATGCAGCA GTCCCGGCAA 
CTCGATCCCA GCCTGTTGGT GAAGCAGCAG ACCCCGCCAT CTCAGCAGCA GCCACTCCAT 
CAGCCAGCCA TGAAATCCTT CCTCGAGAAT GTCATGCCCC ACACTACACC TGAGCTGCAG 
AAAGGGCCAT CACCAATAAA TGCTTTCAGC AACTTCCCGA TAGGCTTGAA CTCAAACTTG 
AATGTAAATA TGGATATGAA CAGTATTAAA GAGCCACAGT CAAGACTAAG GAAGTGGACT 
ACAGTGGACA GCATTTCTGT GAACACATCT TTGGATCAAA ACTCCAGCAA ACATGGTGCT 
ATTTCAAGTG GTTTCAGGCT GGAAGAGTCT CCATTTGTTC CCTATGACTT TATGAACAGC 
AGTACTTCTC CAGCCAGCCC TCCGGGTTCA ATAGGAGATG GCTGGCCACG TGCCAAATCG 
CCTAACGGCT CTAGCAGTGT TAACTGGCCA CCAGAATTTC GCCCTGGTGA ACCATGGAAA 
GGTTATCCAA ACATTGACCC TGAAACTGAC CCTTACGTCA CTCCTGGCAG TGTCATAAAC 
AATCTTTCAA TTAATACTGT GCGGGAAGTT GACCACCTCA GGGACAGGAA CAGTGGGTCA 
TCCTCATCCT TGAACACCAC GCTGCCTTCA ACTAGTGCCT GGTCATCCAT TCGTGCCTCC 
AACTACAACG TTCCCCTCAG CAGTACAGCA CAAAGCACTT CAGCCAGAAA TAGTGATTCC 
AAATTGACAT GGTCTCCTGG TTCAGTTACA AACACCTCTC TGGCTCATGA GCTGTGGAAG 
GTCCCTTTAC CACCTAAAAA CATCACTGCT CCGTCCCGCC CACCTCCGGG ACTCACTGGT 
CAGAAGCCAC CCTTGTCTAC GTGGGATAAC TCTCCCCTTC GTGTAGGTGG AGGATGGGGA 
AATTCTGACG CCAGGTACAC CCCAGGTTCC AGTTGGGGTG AGAGCAGCTC AGGGAGAATA 
ACAAATTGGC TTGTTCTAAA GAACCTCACA CCTCAGATTG ACGGCTCAAC TCTGCGTACT 
CTGTGCATGC AGCATGGCCC ACTGATAACA TTCCACCTGA ACCTCCCACA TGGAAATGCT 
TTGGTCCGTT ACAGTTCAAA AGAAGAGGTA GTGAAGGCAC AAAAGTCTCT GCACATGTGT 
GTATTGGGGA ACACTACTAT TCTTGCTGAG TTTGCCAGTG AAGAGGAGAT CAGCCGTTTC 
TTTGCACAAA GTCAGTCTCT GACCCCTTCT CCCGGCTGGC AGTCTCTCGG GTCCAGCCAG 
AGCCGGCTGG GCTCCCTCGA CTGTTCCCAC TCGTTCTCCA GCCGGACCGA TCTCAATCAC 
TGGAATGGTG CTGGGCTGTC GGGAACTAAC TGTGGAGACC TTCACGGCAC TTCACTCTGG 
GGGACCCCAC ATTATTCCAC AAGCCTGTGG GGTCCTCCCA GCAGCAGCGA CCCCCGGGGA 
ATTAGCAGCC CATCCCCCAT TAATGCTTTT CTTTCTGTTG ACCACCTGGG TGGGGGTGGA 
GAGTCCATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005621456.1
CDS	276..6227
Translation	MQLVAEPGLEARCPGSQDIPLPEEWNRELEAKATKDVERTLSRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNPQQQQQPQPPQQPQQPPQQPPQPPQQQPQPAQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLSSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSPSDSSTNCKNAVVNDSPETEAWPSVPGSDPELASECMDADSASSSESERNITIMASGSTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSPGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLDNNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVGGSSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVMNGTSLSHLSNGEPKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGINGPMGTNFQVNTNKGGGVWESGATNSQNASWGSGNGANSGGSRRGWGTPAQNTGTNIPSVEWSKLPSNQHSNDSANGNGKKFTNGWKSTEEEDQASAASQTTEQNSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACTDKTSPTSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDAAATGMVKSNQWGNSKEEKSAWNDSQKSKQGWGDGQKSNQGWSVSASDNWGETSRSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVTSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPTKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSASAISNKEAGSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASNTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGTMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERSPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPSLLVKQQTPPSQQQPLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_005621399.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005621399.3

ORF Insert Sequence:

ATGCAACTAG TGGCAGAGCC AGGCCTAGAA GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG AGAATTGGAA GCTAAAGCTA CCAAAGACGT AGAAAGGACT 
CTTAGCAGGG ATTTAGTGCA AGAAGAAGAA CAGTTGATGG AAGAAAAGAA AAAGAAAAAA 
GACGACAAGA AAAAGAAGGA AGCTGCTCAA AAGAAGGCCA CTGAACAAAA AATCAAAGTG 
CCAGAACAGA TAAAGCCCAG TGTAAGCCAG CCTCAGCCTG CCAACTCTAA TAACGGCACT 
TCCACAGCAA CCAGCACTAA TAATAATGCC AAGCGAGCCA CAGCCAACAA TCCGCAGCAG 
CAGCAGCAGC CACAGCCGCC ACAGCAGCCA CAGCAGCCGC CGCAGCAGCC ACCGCAGCCG 
CCACAGCAGC AGCCGCAGCC AGCACAGGCC TTGCCTCGGT ATCCTCGGGA AGTACCACCA 
CGATTTCGCC ACCAGGAACA CAAACAGCTT CTAAAGAGGG GTCAGCATTT TCCTGTCATA 
GCAGCAAACC TGGGATCTGC TGTTAAAGTG TTAAGCAGCC AGTCAGAAAG CAGTGCTTTA 
ACAAATCAAC AGCCACAAAA TAACGGAGAG GTGCAGAACA GCAAAAACCA GTCAGATATA 
AACCACAGTA CTTCAGGATC CCATTATGAA AATTCACAGC GGGGACCTGT GTCTTCTCCG 
AGTGACTCTA GCACAAATTG TAAGAATGCT GTTGTAAACG ACTCCCCTGA GACAGAAGCA 
TGGCCCTCAG TCCCTGGCAG TGATCCAGAG TTGGCTTCAG AATGTATGGA TGCTGATTCT 
GCCTCCAGTT CTGAATCAGA GAGAAACATC ACTATCATGG CTTCAGGGAG CACAGGTGGT 
GAAAAAGATG GCCTTCGGAA TAGCACTGGA CTTGGTTCCC AAAACAAGTT TGTGGTTGGT 
AGCAGCAGCA ATAATGTGGG CCATGGAAGT AGTCCTGGGC CATGGGGTTT TTCCCATGGA 
GCCATAATAA GCACATGTCA GGTCTCTGTG GATGCTCCTG AAAGCAAATC AGAAAGTAGC 
AACAATAGAA TGAATGCTTG GGGCACTGTA AGTTCTTCAT CAAATGGAGG GTTAAATCCA 
AGCACTTTGA ATTCAGCTAG CAACCATGGT GCCTGGCCAG TATTAGACAA CAACGGACTT 
GCCCTCAAAG GGCCTGTAGG GAGCGGTAGT TCTGGCATCA ATATTCAGTG TAGTACCATA 
GGCCAGATGC CTAACAATCA GAGTATTAAC TCTAAAGTGG GTGGTTCTTC CACCCATGGT 
ACCTGGGGAA GCCTTCAGGA AACTTGTGAA TCTGAAGTAA GTGGTACACA GAAGGTTTCA 
TTCAGTGGTC AACCTCAGAA TATTACCACT GAGATGACTG GACCAAATAA CACTACTAAC 
TTTATGACCT CTAGTTTACC AAACTCCGGT TCAGTACAGA ATAACGAACT GCCTAGTAAT 
ACAGGGGCCT GGCGTGTGAG CACAATGAAT CATCCTCAGA TACAGGCTCC ATCGGTTATG 
AATGGCACTT CCCTTTCTCA TCTTAGCAAT GGAGAGCCAA AAAGTGGAGG CTCTTACGGT 
ACTACGTGGG GTGCCTATGG TTCTAATTAC TCTGGAGACA AATGTTCGAG CCCTAACGGC 
CAAGCTAATG GTGACACTGT GAATGCAACT CTAATGCAGC CTGGCATAAA TGGGCCTATG 
GGCACTAACT TTCAAGTGAA CACAAATAAA GGAGGAGGTG TGTGGGAGTC TGGGGCAACG 
AATTCCCAGA ATGCATCGTG GGGAAGTGGA AATGGCGCAA ATTCTGGAGG AAGTCGACGA 
GGATGGGGAA CTCCTGCACA AAACACTGGC ACTAATATAC CCAGCGTGGA GTGGAGCAAA 
CTGCCTAGCA ATCAGCATTC CAATGACAGT GCAAATGGCA ATGGTAAGAA GTTTACAAAT 
GGATGGAAAT CTACTGAGGA AGAGGATCAG GCTTCTGCCG CATCTCAGAC AACTGAGCAG 
AACAGTGTGT GGGCCAAAAC AGGAGGTACA GTGGAGAGTG AAGGTAGTAC AGAAAGCACT 
GGACGCCTCG AGGAGAAAGC CACTGGGGAA AATCAGAGTA GAGATCGGAG AAAAATTGAT 
CAGCACACAT TACTCCAAAG CATCGTAAAC AGAACTGACT TGGATCCACG TGTCCTCTCC 
AACTCTGGTT GGGGACAGAC TCCTATTAAG CAGAATACTG CCTGGGATAC GGAAACATCA 
CCTAGAGGGG AAAGAAAGAC TGACAATGGG ACAGAGGCCT GGGGAAGCTC TGCAACACAG 
ACTTTTAACT CAGGGGCATG TACAGATAAG ACTAGCCCTA CTAGTAATGA TACCTCATCT 
GTATCGGGGT GGGGAGATCC CAAACCTGCT CTGAGGTGGG GAGATTCCAA AGGCTCAAGC 
TGCCAGGGGG GATGGGAAGA TGATGCTGCT GCTACAGGAA TGGTCAAGAG CAATCAGTGG 
GGGAACAGCA AAGAAGAGAA GTCCGCATGG AATGATTCGC AAAAGAGCAA ACAGGGATGG 
GGTGATGGAC AGAAATCAAA CCAAGGGTGG TCCGTTTCTG CCAGTGATAA CTGGGGAGAA 
ACGTCAAGGA GTAACCATTG GGGTGAGGCT AATAAGAAAT CTAGCTCAGG AGGTAGTGAC 
AGTGACAGGT CTGTTTCTGG TTGGAATGAA CTTGGTAAAA CTAGTTCTTT TACTTGGGGA 
AATAACATAA ATCCAAATAA TTCGTCAGGA TGGGATGAAT CTTCTAAACC TAATCCTTCC 
CAGGGATGGG GAGATCCTCC AAAGTCTAAT CAGTCTCTAG GTTGGGGAGA TTCGTCAAAG 
CCAGTCACTT CTCCAGACTG GAACAAGCAA CAAGACATTG TCGGGTCTTG GGGAATCCCA 
CCAGCAACAG GCAAACCTCC TGGTACAGGC TGGCTGGGAG GACCTATACC AGCCCCAACA 
AAAGAAGAAG AGCCCACAGG CTGGGAGGAA CCATCCCCAG AATCCATACG TCGCAAAATG 
GAGATCGATG ACGGAACTTC AGCTTGGGGA GATCCGAGCA AATACAACTA CAAAAATGTG 
AACATGTGGA ACAAAAATGT CCCAAATGGC AGCAGCCGTT CAGACCAGCA AGCACAGGTA 
CACCAGTTGC TACCATCTGC AAGTGCCATC TCAAACAAAG AGGCAGGCAG TGGCTCTGGC 
TGGGGTGAGC CCTGGGGGGA GCCTTCTACT CCAGCCACAA CTGTGGATAA TGGTACTTCA 
GCATGGGGTA AACCCATAGA CAGTGGTCCC AGCTGGGGGG AGCCCATTGC TGCAGCATCC 
AACACATCCA CGTGGGGCTC CAGCTCTGTT GGTCCTCAAG CATTAAGCAA ATCTGGGCCA 
AAATCTATGC AAGATGGCTG GTGTGGTGAT GATATGCCAT TGCCTGGAAA TCGCCCCACT 
GGCTGGGAAG AGGAAGAGGA TGTAGAGATT GGAATGTGGA ATAGTAATTC ATCTCAAGAG 
CTTAACTCAT CTTTAAATTG GCCTCCATAT ACAAAGAAAA TGTCATCGAA GGGTCTGAGT 
GGCAAAAAAA GGAGAAGGGA AAGGGGAACC ATGAAAGGTG GAAACAAACA AGAAGAAGCA 
TGGATAAATC CATTCGTTAA ACAGTTTTCA AATATCAGTT TTTCGAGAGA CTCACCCGAA 
GAAAATGTAC AGAGCAATAA GATGGATCTT TCCGGAGGAA TGTTACAGGA CAAACGAATG 
GAGATAGATA AACATAGCCT AAATATTGGT GATTACAATC GAACGGTCGG GAAAGGCCCT 
GGTTCTCGGC CTCAGATTTC CAAAGAGTCT TCCATGGAGC GCAGCCCTTA CTTTGATAAG 
GATGGCATTG TAGCAGATGA ATCCCAAAAC ATGCAGTTTA TGTCCAGTCA AAGCATGAAG 
CTTCCCCCTT CAAATAGTGC ACTACCTAAC CAGGCCCTTG GCTCCATAGC AGGGCTGGGT 
ATGCAAAACT TGAATTCTGT TAGACAGAAT GGCAATCCCA GTATGTTTGG TGTTGGAAAC 
ACAGCAGCAC AACCCCGGGG CATGCAGCAG CCTCCAGCAC AACCTCTTAG TTCATCTCAG 
CCTAATCTTC GTGCTCAAGT GCCTCCTCCA TTACTCTCCC CTCAGGTTCC AGTTTCATTG 
CTGAAGTATG CACCAAACAA CGGTGGCCTG AATCCGCTCT TTGGCCCTCA ACAGGTAGCC 
ATGCTGAACC AGCTATCCCA ACTAAACCAG CTTTCTCAGA TCTCCCAGTT ACAGAGATTG 
TTAGCGCAGC AGCAAAGGGC GCAGAGTCAG AGAAGCGTGC CTTCTGGGAA CCGGCAGCAG 
CAAGACCAGC AGGGTCGACC TCTTAGCGTG CAGCAGCAGA TGATGCAGCA GTCCCGGCAA 
CTCGATCCCA GCCTGTTGGT GAAGCAGCAG ACCCCGCCAT CTCAGCAGCA GCCACTCCAT 
CAGCCAGCCA TGAAATCCTT CCTCGAGAAT GTCATGCCCC ACACTACACC TGAGCTGCAG 
AAAGGGCCAT CACCAATAAA TGCTTTCAGC AACTTCCCGA TAGGCTTGAA CTCAAACTTG 
AATGTAAATA TGGATATGAA CAGTATTAAA GAGCCACAGT CAAGACTAAG GAAGTGGACT 
ACAGTGGACA GCATTTCTGT GAACACATCT TTGGATCAAA ACTCCAGCAA ACATGGTGCT 
ATTTCAAGTG GTTTCAGGCT GGAAGAGTCT CCATTTGTTC CCTATGACTT TATGAACAGC 
AGTACTTCTC CAGCCAGCCC TCCGGGTTCA ATAGGAGATG GCTGGCCACG TGCCAAATCG 
CCTAACGGCT CTAGCAGTGT TAACTGGCCA CCAGAATTTC GCCCTGGTGA ACCATGGAAA 
GGTTATCCAA ACATTGACCC TGAAACTGAC CCTTACGTCA CTCCTGGCAG TGTCATAAAC 
AATCTTTCAA TTAATACTGT GCGGGAAGTT GACCACCTCA GGGACAGGAA CAGTGGGTCA 
TCCTCATCCT TGAACACCAC GCTGCCTTCA ACTAGTGCCT GGTCATCCAT TCGTGCCTCC 
AACTACAACG TTCCCCTCAG CAGTACAGCA CAAAGCACTT CAGCCAGAAA TAGTGATTCC 
AAATTGACAT GGTCTCCTGG TTCAGTTACA AACACCTCTC TGGCTCATGA GCTGTGGAAG 
GTCCCTTTAC CACCTAAAAA CATCACTGCT CCGTCCCGCC CACCTCCGGG ACTCACTGGT 
CAGAAGCCAC CCTTGTCTAC GTGGGATAAC TCTCCCCTTC GTGTAGGTGG AGGATGGGGA 
AATTCTGACG CCAGGTACAC CCCAGGTTCC AGTTGGGGTG AGAGCAGCTC AGGGAGAATA 
ACAAATTGGC TTGTTCTAAA GAACCTCACA CCTCAGATTG ACGGCTCAAC TCTGCGTACT 
CTGTGCATGC AGCATGGCCC ACTGATAACA TTCCACCTGA ACCTCCCACA TGGAAATGCT 
TTGGTCCGTT ACAGTTCAAA AGAAGAGGTA GTGAAGGCAC AAAAGTCTCT GCACATGTGT 
GTATTGGGGA ACACTACTAT TCTTGCTGAG TTTGCCAGTG AAGAGGAGAT CAGCCGTTTC 
TTTGCACAAA GTCAGTCTCT GACCCCTTCT CCCGGCTGGC AGTCTCTCGG GTCCAGCCAG 
AGCCGGCTGG GCTCCCTCGA CTGTTCCCAC TCGTTCTCCA GCCGGACCGA TCTCAATCAC 
TGGAATGGTG CTGGGCTGTC GGGAACTAAC TGTGGAGACC TTCACGGCAC TTCACTCTGG 
GGGACCCCAC ATTATTCCAC AAGCCTGTGG GGTCCTCCCA GCAGCAGCGA CCCCCGGGGA 
ATTAGCAGCC CATCCCCCAT TAATGCTTTT CTTTCTGTTG ACCACCTGGG TGGGGGTGGA 
GAGTCCATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_005621399.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005621456.1 Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X1, mRNA.

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CloneID	OCe07956
Clone ID Related Accession (Same CDS sequence)	XM_005621399.3 , XM_005621399.2
Accession Version	XM_005621399.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5952bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	trinucleotide repeat-containing gene 6A protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005621399.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACTAG TGGCAGAGCC AGGCCTAGAA GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG AGAATTGGAA GCTAAAGCTA CCAAAGACGT AGAAAGGACT 
CTTAGCAGGG ATTTAGTGCA AGAAGAAGAA CAGTTGATGG AAGAAAAGAA AAAGAAAAAA 
GACGACAAGA AAAAGAAGGA AGCTGCTCAA AAGAAGGCCA CTGAACAAAA AATCAAAGTG 
CCAGAACAGA TAAAGCCCAG TGTAAGCCAG CCTCAGCCTG CCAACTCTAA TAACGGCACT 
TCCACAGCAA CCAGCACTAA TAATAATGCC AAGCGAGCCA CAGCCAACAA TCCGCAGCAG 
CAGCAGCAGC CACAGCCGCC ACAGCAGCCA CAGCAGCCGC CGCAGCAGCC ACCGCAGCCG 
CCACAGCAGC AGCCGCAGCC AGCACAGGCC TTGCCTCGGT ATCCTCGGGA AGTACCACCA 
CGATTTCGCC ACCAGGAACA CAAACAGCTT CTAAAGAGGG GTCAGCATTT TCCTGTCATA 
GCAGCAAACC TGGGATCTGC TGTTAAAGTG TTAAGCAGCC AGTCAGAAAG CAGTGCTTTA 
ACAAATCAAC AGCCACAAAA TAACGGAGAG GTGCAGAACA GCAAAAACCA GTCAGATATA 
AACCACAGTA CTTCAGGATC CCATTATGAA AATTCACAGC GGGGACCTGT GTCTTCTCCG 
AGTGACTCTA GCACAAATTG TAAGAATGCT GTTGTAAACG ACTCCCCTGA GACAGAAGCA 
TGGCCCTCAG TCCCTGGCAG TGATCCAGAG TTGGCTTCAG AATGTATGGA TGCTGATTCT 
GCCTCCAGTT CTGAATCAGA GAGAAACATC ACTATCATGG CTTCAGGGAG CACAGGTGGT 
GAAAAAGATG GCCTTCGGAA TAGCACTGGA CTTGGTTCCC AAAACAAGTT TGTGGTTGGT 
AGCAGCAGCA ATAATGTGGG CCATGGAAGT AGTCCTGGGC CATGGGGTTT TTCCCATGGA 
GCCATAATAA GCACATGTCA GGTCTCTGTG GATGCTCCTG AAAGCAAATC AGAAAGTAGC 
AACAATAGAA TGAATGCTTG GGGCACTGTA AGTTCTTCAT CAAATGGAGG GTTAAATCCA 
AGCACTTTGA ATTCAGCTAG CAACCATGGT GCCTGGCCAG TATTAGACAA CAACGGACTT 
GCCCTCAAAG GGCCTGTAGG GAGCGGTAGT TCTGGCATCA ATATTCAGTG TAGTACCATA 
GGCCAGATGC CTAACAATCA GAGTATTAAC TCTAAAGTGG GTGGTTCTTC CACCCATGGT 
ACCTGGGGAA GCCTTCAGGA AACTTGTGAA TCTGAAGTAA GTGGTACACA GAAGGTTTCA 
TTCAGTGGTC AACCTCAGAA TATTACCACT GAGATGACTG GACCAAATAA CACTACTAAC 
TTTATGACCT CTAGTTTACC AAACTCCGGT TCAGTACAGA ATAACGAACT GCCTAGTAAT 
ACAGGGGCCT GGCGTGTGAG CACAATGAAT CATCCTCAGA TACAGGCTCC ATCGGTTATG 
AATGGCACTT CCCTTTCTCA TCTTAGCAAT GGAGAGCCAA AAAGTGGAGG CTCTTACGGT 
ACTACGTGGG GTGCCTATGG TTCTAATTAC TCTGGAGACA AATGTTCGAG CCCTAACGGC 
CAAGCTAATG GTGACACTGT GAATGCAACT CTAATGCAGC CTGGCATAAA TGGGCCTATG 
GGCACTAACT TTCAAGTGAA CACAAATAAA GGAGGAGGTG TGTGGGAGTC TGGGGCAACG 
AATTCCCAGA ATGCATCGTG GGGAAGTGGA AATGGCGCAA ATTCTGGAGG AAGTCGACGA 
GGATGGGGAA CTCCTGCACA AAACACTGGC ACTAATATAC CCAGCGTGGA GTGGAGCAAA 
CTGCCTAGCA ATCAGCATTC CAATGACAGT GCAAATGGCA ATGGTAAGAA GTTTACAAAT 
GGATGGAAAT CTACTGAGGA AGAGGATCAG GCTTCTGCCG CATCTCAGAC AACTGAGCAG 
AACAGTGTGT GGGCCAAAAC AGGAGGTACA GTGGAGAGTG AAGGTAGTAC AGAAAGCACT 
GGACGCCTCG AGGAGAAAGC CACTGGGGAA AATCAGAGTA GAGATCGGAG AAAAATTGAT 
CAGCACACAT TACTCCAAAG CATCGTAAAC AGAACTGACT TGGATCCACG TGTCCTCTCC 
AACTCTGGTT GGGGACAGAC TCCTATTAAG CAGAATACTG CCTGGGATAC GGAAACATCA 
CCTAGAGGGG AAAGAAAGAC TGACAATGGG ACAGAGGCCT GGGGAAGCTC TGCAACACAG 
ACTTTTAACT CAGGGGCATG TACAGATAAG ACTAGCCCTA CTAGTAATGA TACCTCATCT 
GTATCGGGGT GGGGAGATCC CAAACCTGCT CTGAGGTGGG GAGATTCCAA AGGCTCAAGC 
TGCCAGGGGG GATGGGAAGA TGATGCTGCT GCTACAGGAA TGGTCAAGAG CAATCAGTGG 
GGGAACAGCA AAGAAGAGAA GTCCGCATGG AATGATTCGC AAAAGAGCAA ACAGGGATGG 
GGTGATGGAC AGAAATCAAA CCAAGGGTGG TCCGTTTCTG CCAGTGATAA CTGGGGAGAA 
ACGTCAAGGA GTAACCATTG GGGTGAGGCT AATAAGAAAT CTAGCTCAGG AGGTAGTGAC 
AGTGACAGGT CTGTTTCTGG TTGGAATGAA CTTGGTAAAA CTAGTTCTTT TACTTGGGGA 
AATAACATAA ATCCAAATAA TTCGTCAGGA TGGGATGAAT CTTCTAAACC TAATCCTTCC 
CAGGGATGGG GAGATCCTCC AAAGTCTAAT CAGTCTCTAG GTTGGGGAGA TTCGTCAAAG 
CCAGTCACTT CTCCAGACTG GAACAAGCAA CAAGACATTG TCGGGTCTTG GGGAATCCCA 
CCAGCAACAG GCAAACCTCC TGGTACAGGC TGGCTGGGAG GACCTATACC AGCCCCAACA 
AAAGAAGAAG AGCCCACAGG CTGGGAGGAA CCATCCCCAG AATCCATACG TCGCAAAATG 
GAGATCGATG ACGGAACTTC AGCTTGGGGA GATCCGAGCA AATACAACTA CAAAAATGTG 
AACATGTGGA ACAAAAATGT CCCAAATGGC AGCAGCCGTT CAGACCAGCA AGCACAGGTA 
CACCAGTTGC TACCATCTGC AAGTGCCATC TCAAACAAAG AGGCAGGCAG TGGCTCTGGC 
TGGGGTGAGC CCTGGGGGGA GCCTTCTACT CCAGCCACAA CTGTGGATAA TGGTACTTCA 
GCATGGGGTA AACCCATAGA CAGTGGTCCC AGCTGGGGGG AGCCCATTGC TGCAGCATCC 
AACACATCCA CGTGGGGCTC CAGCTCTGTT GGTCCTCAAG CATTAAGCAA ATCTGGGCCA 
AAATCTATGC AAGATGGCTG GTGTGGTGAT GATATGCCAT TGCCTGGAAA TCGCCCCACT 
GGCTGGGAAG AGGAAGAGGA TGTAGAGATT GGAATGTGGA ATAGTAATTC ATCTCAAGAG 
CTTAACTCAT CTTTAAATTG GCCTCCATAT ACAAAGAAAA TGTCATCGAA GGGTCTGAGT 
GGCAAAAAAA GGAGAAGGGA AAGGGGAACC ATGAAAGGTG GAAACAAACA AGAAGAAGCA 
TGGATAAATC CATTCGTTAA ACAGTTTTCA AATATCAGTT TTTCGAGAGA CTCACCCGAA 
GAAAATGTAC AGAGCAATAA GATGGATCTT TCCGGAGGAA TGTTACAGGA CAAACGAATG 
GAGATAGATA AACATAGCCT AAATATTGGT GATTACAATC GAACGGTCGG GAAAGGCCCT 
GGTTCTCGGC CTCAGATTTC CAAAGAGTCT TCCATGGAGC GCAGCCCTTA CTTTGATAAG 
GATGGCATTG TAGCAGATGA ATCCCAAAAC ATGCAGTTTA TGTCCAGTCA AAGCATGAAG 
CTTCCCCCTT CAAATAGTGC ACTACCTAAC CAGGCCCTTG GCTCCATAGC AGGGCTGGGT 
ATGCAAAACT TGAATTCTGT TAGACAGAAT GGCAATCCCA GTATGTTTGG TGTTGGAAAC 
ACAGCAGCAC AACCCCGGGG CATGCAGCAG CCTCCAGCAC AACCTCTTAG TTCATCTCAG 
CCTAATCTTC GTGCTCAAGT GCCTCCTCCA TTACTCTCCC CTCAGGTTCC AGTTTCATTG 
CTGAAGTATG CACCAAACAA CGGTGGCCTG AATCCGCTCT TTGGCCCTCA ACAGGTAGCC 
ATGCTGAACC AGCTATCCCA ACTAAACCAG CTTTCTCAGA TCTCCCAGTT ACAGAGATTG 
TTAGCGCAGC AGCAAAGGGC GCAGAGTCAG AGAAGCGTGC CTTCTGGGAA CCGGCAGCAG 
CAAGACCAGC AGGGTCGACC TCTTAGCGTG CAGCAGCAGA TGATGCAGCA GTCCCGGCAA 
CTCGATCCCA GCCTGTTGGT GAAGCAGCAG ACCCCGCCAT CTCAGCAGCA GCCACTCCAT 
CAGCCAGCCA TGAAATCCTT CCTCGAGAAT GTCATGCCCC ACACTACACC TGAGCTGCAG 
AAAGGGCCAT CACCAATAAA TGCTTTCAGC AACTTCCCGA TAGGCTTGAA CTCAAACTTG 
AATGTAAATA TGGATATGAA CAGTATTAAA GAGCCACAGT CAAGACTAAG GAAGTGGACT 
ACAGTGGACA GCATTTCTGT GAACACATCT TTGGATCAAA ACTCCAGCAA ACATGGTGCT 
ATTTCAAGTG GTTTCAGGCT GGAAGAGTCT CCATTTGTTC CCTATGACTT TATGAACAGC 
AGTACTTCTC CAGCCAGCCC TCCGGGTTCA ATAGGAGATG GCTGGCCACG TGCCAAATCG 
CCTAACGGCT CTAGCAGTGT TAACTGGCCA CCAGAATTTC GCCCTGGTGA ACCATGGAAA 
GGTTATCCAA ACATTGACCC TGAAACTGAC CCTTACGTCA CTCCTGGCAG TGTCATAAAC 
AATCTTTCAA TTAATACTGT GCGGGAAGTT GACCACCTCA GGGACAGGAA CAGTGGGTCA 
TCCTCATCCT TGAACACCAC GCTGCCTTCA ACTAGTGCCT GGTCATCCAT TCGTGCCTCC 
AACTACAACG TTCCCCTCAG CAGTACAGCA CAAAGCACTT CAGCCAGAAA TAGTGATTCC 
AAATTGACAT GGTCTCCTGG TTCAGTTACA AACACCTCTC TGGCTCATGA GCTGTGGAAG 
GTCCCTTTAC CACCTAAAAA CATCACTGCT CCGTCCCGCC CACCTCCGGG ACTCACTGGT 
CAGAAGCCAC CCTTGTCTAC GTGGGATAAC TCTCCCCTTC GTGTAGGTGG AGGATGGGGA 
AATTCTGACG CCAGGTACAC CCCAGGTTCC AGTTGGGGTG AGAGCAGCTC AGGGAGAATA 
ACAAATTGGC TTGTTCTAAA GAACCTCACA CCTCAGATTG ACGGCTCAAC TCTGCGTACT 
CTGTGCATGC AGCATGGCCC ACTGATAACA TTCCACCTGA ACCTCCCACA TGGAAATGCT 
TTGGTCCGTT ACAGTTCAAA AGAAGAGGTA GTGAAGGCAC AAAAGTCTCT GCACATGTGT 
GTATTGGGGA ACACTACTAT TCTTGCTGAG TTTGCCAGTG AAGAGGAGAT CAGCCGTTTC 
TTTGCACAAA GTCAGTCTCT GACCCCTTCT CCCGGCTGGC AGTCTCTCGG GTCCAGCCAG 
AGCCGGCTGG GCTCCCTCGA CTGTTCCCAC TCGTTCTCCA GCCGGACCGA TCTCAATCAC 
TGGAATGGTG CTGGGCTGTC GGGAACTAAC TGTGGAGACC TTCACGGCAC TTCACTCTGG 
GGGACCCCAC ATTATTCCAC AAGCCTGTGG GGTCCTCCCA GCAGCAGCGA CCCCCGGGGA 
ATTAGCAGCC CATCCCCCAT TAATGCTTTT CTTTCTGTTG ACCACCTGGG TGGGGGTGGA 
GAGTCCATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005621456.1
CDS	283..6234
Translation	MQLVAEPGLEARCPGSQDIPLPEEWNRELEAKATKDVERTLSRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNPQQQQQPQPPQQPQQPPQQPPQPPQQQPQPAQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLSSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSPSDSSTNCKNAVVNDSPETEAWPSVPGSDPELASECMDADSASSSESERNITIMASGSTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSPGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLDNNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVGGSSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVMNGTSLSHLSNGEPKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGINGPMGTNFQVNTNKGGGVWESGATNSQNASWGSGNGANSGGSRRGWGTPAQNTGTNIPSVEWSKLPSNQHSNDSANGNGKKFTNGWKSTEEEDQASAASQTTEQNSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACTDKTSPTSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDAAATGMVKSNQWGNSKEEKSAWNDSQKSKQGWGDGQKSNQGWSVSASDNWGETSRSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVTSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPTKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSASAISNKEAGSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASNTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGTMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERSPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPSLLVKQQTPPSQQQPLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_005621399.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005621399.2

ORF Insert Sequence:

ATGCAACTAG TGGCAGAGCC AGGCCTAGAA GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG AGAATTGGAA GCTAAAGCTA CCAAAGACGT AGAAAGGACT 
CTTAGCAGGG ATTTAGTGCA AGAAGAAGAA CAGTTGATGG AAGAAAAGAA AAAGAAAAAA 
GACGACAAGA AAAAGAAGGA AGCTGCTCAA AAGAAGGCCA CTGAACAAAA AATCAAAGTG 
CCAGAACAGA TAAAGCCCAG TGTAAGCCAG CCTCAGCCTG CCAACTCTAA TAACGGCACT 
TCCACAGCAA CCAGCACTAA TAATAATGCC AAGCGAGCCA CAGCCAACAA TCCGCAGCAG 
CAGCAGCAGC CACAGCCGCC ACAGCAGCCA CAGCAGCCGC CGCAGCAGCC ACCGCAGCCG 
CCACAGCAGC AGCCGCAGCC AGCACAGGCC TTGCCTCGGT ATCCTCGGGA AGTACCACCA 
CGATTTCGCC ACCAGGAACA CAAACAGCTT CTAAAGAGGG GTCAGCATTT TCCTGTCATA 
GCAGCAAACC TGGGATCTGC TGTTAAAGTG TTAAGCAGCC AGTCAGAAAG CAGTGCTTTA 
ACAAATCAAC AGCCACAAAA TAACGGAGAG GTGCAGAACA GCAAAAACCA GTCAGATATA 
AACCACAGTA CTTCAGGATC CCATTATGAA AATTCACAGC GGGGACCTGT GTCTTCTCCG 
AGTGACTCTA GCACAAATTG TAAGAATGCT GTTGTAAACG ACTCCCCTGA GACAGAAGCA 
TGGCCCTCAG TCCCTGGCAG TGATCCAGAG TTGGCTTCAG AATGTATGGA TGCTGATTCT 
GCCTCCAGTT CTGAATCAGA GAGAAACATC ACTATCATGG CTTCAGGGAG CACAGGTGGT 
GAAAAAGATG GCCTTCGGAA TAGCACTGGA CTTGGTTCCC AAAACAAGTT TGTGGTTGGT 
AGCAGCAGCA ATAATGTGGG CCATGGAAGT AGTCCTGGGC CATGGGGTTT TTCCCATGGA 
GCCATAATAA GCACATGTCA GGTCTCTGTG GATGCTCCTG AAAGCAAATC AGAAAGTAGC 
AACAATAGAA TGAATGCTTG GGGCACTGTA AGTTCTTCAT CAAATGGAGG GTTAAATCCA 
AGCACTTTGA ATTCAGCTAG CAACCATGGT GCCTGGCCAG TATTAGACAA CAACGGACTT 
GCCCTCAAAG GGCCTGTAGG GAGCGGTAGT TCTGGCATCA ATATTCAGTG TAGTACCATA 
GGCCAGATGC CTAACAATCA GAGTATTAAC TCTAAAGTGG GTGGTTCTTC CACCCATGGT 
ACCTGGGGAA GCCTTCAGGA AACTTGTGAA TCTGAAGTAA GTGGTACACA GAAGGTTTCA 
TTCAGTGGTC AACCTCAGAA TATTACCACT GAGATGACTG GACCAAATAA CACTACTAAC 
TTTATGACCT CTAGTTTACC AAACTCCGGT TCAGTACAGA ATAACGAACT GCCTAGTAAT 
ACAGGGGCCT GGCGTGTGAG CACAATGAAT CATCCTCAGA TACAGGCTCC ATCGGTTATG 
AATGGCACTT CCCTTTCTCA TCTTAGCAAT GGAGAGCCAA AAAGTGGAGG CTCTTACGGT 
ACTACGTGGG GTGCCTATGG TTCTAATTAC TCTGGAGACA AATGTTCGAG CCCTAACGGC 
CAAGCTAATG GTGACACTGT GAATGCAACT CTAATGCAGC CTGGCATAAA TGGGCCTATG 
GGCACTAACT TTCAAGTGAA CACAAATAAA GGAGGAGGTG TGTGGGAGTC TGGGGCAACG 
AATTCCCAGA ATGCATCGTG GGGAAGTGGA AATGGCGCAA ATTCTGGAGG AAGTCGACGA 
GGATGGGGAA CTCCTGCACA AAACACTGGC ACTAATATAC CCAGCGTGGA GTGGAGCAAA 
CTGCCTAGCA ATCAGCATTC CAATGACAGT GCAAATGGCA ATGGTAAGAA GTTTACAAAT 
GGATGGAAAT CTACTGAGGA AGAGGATCAG GCTTCTGCCG CATCTCAGAC AACTGAGCAG 
AACAGTGTGT GGGCCAAAAC AGGAGGTACA GTGGAGAGTG AAGGTAGTAC AGAAAGCACT 
GGACGCCTCG AGGAGAAAGC CACTGGGGAA AATCAGAGTA GAGATCGGAG AAAAATTGAT 
CAGCACACAT TACTCCAAAG CATCGTAAAC AGAACTGACT TGGATCCACG TGTCCTCTCC 
AACTCTGGTT GGGGACAGAC TCCTATTAAG CAGAATACTG CCTGGGATAC GGAAACATCA 
CCTAGAGGGG AAAGAAAGAC TGACAATGGG ACAGAGGCCT GGGGAAGCTC TGCAACACAG 
ACTTTTAACT CAGGGGCATG TACAGATAAG ACTAGCCCTA CTAGTAATGA TACCTCATCT 
GTATCGGGGT GGGGAGATCC CAAACCTGCT CTGAGGTGGG GAGATTCCAA AGGCTCAAGC 
TGCCAGGGGG GATGGGAAGA TGATGCTGCT GCTACAGGAA TGGTCAAGAG CAATCAGTGG 
GGGAACAGCA AAGAAGAGAA GTCCGCATGG AATGATTCGC AAAAGAGCAA ACAGGGATGG 
GGTGATGGAC AGAAATCAAA CCAAGGGTGG TCCGTTTCTG CCAGTGATAA CTGGGGAGAA 
ACGTCAAGGA GTAACCATTG GGGTGAGGCT AATAAGAAAT CTAGCTCAGG AGGTAGTGAC 
AGTGACAGGT CTGTTTCTGG TTGGAATGAA CTTGGTAAAA CTAGTTCTTT TACTTGGGGA 
AATAACATAA ATCCAAATAA TTCGTCAGGA TGGGATGAAT CTTCTAAACC TAATCCTTCC 
CAGGGATGGG GAGATCCTCC AAAGTCTAAT CAGTCTCTAG GTTGGGGAGA TTCGTCAAAG 
CCAGTCACTT CTCCAGACTG GAACAAGCAA CAAGACATTG TCGGGTCTTG GGGAATCCCA 
CCAGCAACAG GCAAACCTCC TGGTACAGGC TGGCTGGGAG GACCTATACC AGCCCCAACA 
AAAGAAGAAG AGCCCACAGG CTGGGAGGAA CCATCCCCAG AATCCATACG TCGCAAAATG 
GAGATCGATG ACGGAACTTC AGCTTGGGGA GATCCGAGCA AATACAACTA CAAAAATGTG 
AACATGTGGA ACAAAAATGT CCCAAATGGC AGCAGCCGTT CAGACCAGCA AGCACAGGTA 
CACCAGTTGC TACCATCTGC AAGTGCCATC TCAAACAAAG AGGCAGGCAG TGGCTCTGGC 
TGGGGTGAGC CCTGGGGGGA GCCTTCTACT CCAGCCACAA CTGTGGATAA TGGTACTTCA 
GCATGGGGTA AACCCATAGA CAGTGGTCCC AGCTGGGGGG AGCCCATTGC TGCAGCATCC 
AACACATCCA CGTGGGGCTC CAGCTCTGTT GGTCCTCAAG CATTAAGCAA ATCTGGGCCA 
AAATCTATGC AAGATGGCTG GTGTGGTGAT GATATGCCAT TGCCTGGAAA TCGCCCCACT 
GGCTGGGAAG AGGAAGAGGA TGTAGAGATT GGAATGTGGA ATAGTAATTC ATCTCAAGAG 
CTTAACTCAT CTTTAAATTG GCCTCCATAT ACAAAGAAAA TGTCATCGAA GGGTCTGAGT 
GGCAAAAAAA GGAGAAGGGA AAGGGGAACC ATGAAAGGTG GAAACAAACA AGAAGAAGCA 
TGGATAAATC CATTCGTTAA ACAGTTTTCA AATATCAGTT TTTCGAGAGA CTCACCCGAA 
GAAAATGTAC AGAGCAATAA GATGGATCTT TCCGGAGGAA TGTTACAGGA CAAACGAATG 
GAGATAGATA AACATAGCCT AAATATTGGT GATTACAATC GAACGGTCGG GAAAGGCCCT 
GGTTCTCGGC CTCAGATTTC CAAAGAGTCT TCCATGGAGC GCAGCCCTTA CTTTGATAAG 
GATGGCATTG TAGCAGATGA ATCCCAAAAC ATGCAGTTTA TGTCCAGTCA AAGCATGAAG 
CTTCCCCCTT CAAATAGTGC ACTACCTAAC CAGGCCCTTG GCTCCATAGC AGGGCTGGGT 
ATGCAAAACT TGAATTCTGT TAGACAGAAT GGCAATCCCA GTATGTTTGG TGTTGGAAAC 
ACAGCAGCAC AACCCCGGGG CATGCAGCAG CCTCCAGCAC AACCTCTTAG TTCATCTCAG 
CCTAATCTTC GTGCTCAAGT GCCTCCTCCA TTACTCTCCC CTCAGGTTCC AGTTTCATTG 
CTGAAGTATG CACCAAACAA CGGTGGCCTG AATCCGCTCT TTGGCCCTCA ACAGGTAGCC 
ATGCTGAACC AGCTATCCCA ACTAAACCAG CTTTCTCAGA TCTCCCAGTT ACAGAGATTG 
TTAGCGCAGC AGCAAAGGGC GCAGAGTCAG AGAAGCGTGC CTTCTGGGAA CCGGCAGCAG 
CAAGACCAGC AGGGTCGACC TCTTAGCGTG CAGCAGCAGA TGATGCAGCA GTCCCGGCAA 
CTCGATCCCA GCCTGTTGGT GAAGCAGCAG ACCCCGCCAT CTCAGCAGCA GCCACTCCAT 
CAGCCAGCCA TGAAATCCTT CCTCGAGAAT GTCATGCCCC ACACTACACC TGAGCTGCAG 
AAAGGGCCAT CACCAATAAA TGCTTTCAGC AACTTCCCGA TAGGCTTGAA CTCAAACTTG 
AATGTAAATA TGGATATGAA CAGTATTAAA GAGCCACAGT CAAGACTAAG GAAGTGGACT 
ACAGTGGACA GCATTTCTGT GAACACATCT TTGGATCAAA ACTCCAGCAA ACATGGTGCT 
ATTTCAAGTG GTTTCAGGCT GGAAGAGTCT CCATTTGTTC CCTATGACTT TATGAACAGC 
AGTACTTCTC CAGCCAGCCC TCCGGGTTCA ATAGGAGATG GCTGGCCACG TGCCAAATCG 
CCTAACGGCT CTAGCAGTGT TAACTGGCCA CCAGAATTTC GCCCTGGTGA ACCATGGAAA 
GGTTATCCAA ACATTGACCC TGAAACTGAC CCTTACGTCA CTCCTGGCAG TGTCATAAAC 
AATCTTTCAA TTAATACTGT GCGGGAAGTT GACCACCTCA GGGACAGGAA CAGTGGGTCA 
TCCTCATCCT TGAACACCAC GCTGCCTTCA ACTAGTGCCT GGTCATCCAT TCGTGCCTCC 
AACTACAACG TTCCCCTCAG CAGTACAGCA CAAAGCACTT CAGCCAGAAA TAGTGATTCC 
AAATTGACAT GGTCTCCTGG TTCAGTTACA AACACCTCTC TGGCTCATGA GCTGTGGAAG 
GTCCCTTTAC CACCTAAAAA CATCACTGCT CCGTCCCGCC CACCTCCGGG ACTCACTGGT 
CAGAAGCCAC CCTTGTCTAC GTGGGATAAC TCTCCCCTTC GTGTAGGTGG AGGATGGGGA 
AATTCTGACG CCAGGTACAC CCCAGGTTCC AGTTGGGGTG AGAGCAGCTC AGGGAGAATA 
ACAAATTGGC TTGTTCTAAA GAACCTCACA CCTCAGATTG ACGGCTCAAC TCTGCGTACT 
CTGTGCATGC AGCATGGCCC ACTGATAACA TTCCACCTGA ACCTCCCACA TGGAAATGCT 
TTGGTCCGTT ACAGTTCAAA AGAAGAGGTA GTGAAGGCAC AAAAGTCTCT GCACATGTGT 
GTATTGGGGA ACACTACTAT TCTTGCTGAG TTTGCCAGTG AAGAGGAGAT CAGCCGTTTC 
TTTGCACAAA GTCAGTCTCT GACCCCTTCT CCCGGCTGGC AGTCTCTCGG GTCCAGCCAG 
AGCCGGCTGG GCTCCCTCGA CTGTTCCCAC TCGTTCTCCA GCCGGACCGA TCTCAATCAC 
TGGAATGGTG CTGGGCTGTC GGGAACTAAC TGTGGAGACC TTCACGGCAC TTCACTCTGG 
GGGACCCCAC ATTATTCCAC AAGCCTGTGG GGTCCTCCCA GCAGCAGCGA CCCCCGGGGA 
ATTAGCAGCC CATCCCCCAT TAATGCTTTT CTTTCTGTTG ACCACCTGGG TGGGGGTGGA 
GAGTCCATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_022419936.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022275644.1 Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X2, mRNA.

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CloneID	OCe07957
Clone ID Related Accession (Same CDS sequence)	XM_022419936.1
Accession Version	XM_022419936.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5919bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	trinucleotide repeat-containing gene 6A protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACTAG TGGCAGAGCC AGGCCTAGAA GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG AGAATTGGAA GCTAAAGCTA CCAAAGACGT AGAAAGGACT 
CTTAGCAGGG ATTTAGTGCA AGAAGAAGAA CAGTTGATGG AAGAAAAGAA AAAGAAAAAA 
GACGACAAGA AAAAGAAGGA AGCTGCTCAA AAGAAGGCCA CTGAACAAAA AATCAAAGTG 
CCAGAACAGA TAAAGCCCAG TGTAAGCCAG CCTCAGCCTG CCAACTCTAA TAACGGCACT 
TCCACAGCAA CCAGCACTAA TAATAATGCC AAGCGAGCCA CAGCCAACAA TCCGCAGCAG 
CAGCAGCAGC CACAGCCGCC ACAGCAGCCA CAGCAGCCGC CGCAGCAGCC ACCGCAGCCG 
CCACAGCAGC AGCCGCAGCC AGCACAGGCC TTGCCTCGGT ATCCTCGGGA AGTACCACCA 
CGATTTCGCC ACCAGGAACA CAAACAGCTT CTAAAGAGGG GTCAGCATTT TCCTGTCATA 
GCAGCAAACC TGGGATCTGC TGTTAAAGTG TTAAGCAGCC AGTCAGAAAG CAGTGCTTTA 
ACAAATCAAC AGCCACAAAA TAACGGAGAG GTGCAGAACA GCAAAAACCA GTCAGATATA 
AACCACAGTA CTTCAGGATC CCATTATGAA AATTCACAGC GGGGACCTGT GTCTTCTCCG 
AGTGACTCTA GCACAAATTG TAAGAATGCT GTTGTAAACG ACTCCCCTGA GACAGAAGCA 
TGGCCCTCAG TCCCTGGCAG TGATCCAGAG TTGGCTTCAG AATGTATGGA TGCTGATTCT 
GCCTCCAGTT CTGAATCAGA GAGAAACATC ACTATCATGG CTTCAGGGAG CACAGGTGGT 
GAAAAAGATG GCCTTCGGAA TAGCACTGGA CTTGGTTCCC AAAACAAGTT TGTGGTTGGT 
AGCAGCAGCA ATAATGTGGG CCATGGAAGT AGTCCTGGGC CATGGGGTTT TTCCCATGGA 
GCCATAATAA GCACATGTCA GGTCTCTGTG GATGCTCCTG AAAGCAAATC AGAAAGTAGC 
AACAATAGAA TGAATGCTTG GGGCACTGTA AGTTCTTCAT CAAATGGAGG GTTAAATCCA 
AGCACTTTGA ATTCAGCTAG CAACCATGGT GCCTGGCCAG TATTAGACAA CAACGGACTT 
GCCCTCAAAG GGCCTGTAGG GAGCGGTAGT TCTGGCATCA ATATTCAGTG TAGTACCATA 
GGCCAGATGC CTAACAATCA GAGTATTAAC TCTAAAGTGG GTGGTTCTTC CACCCATGGT 
ACCTGGGGAA GCCTTCAGGA AACTTGTGAA TCTGAAGTAA GTGGTACACA GAAGGTTTCA 
TTCAGTGGTC AACCTCAGAA TATTACCACT GAGATGACTG GACCAAATAA CACTACTAAC 
TTTATGACCT CTAGTTTACC AAACTCCGGT TCAGTACAGA ATAACGAACT GCCTAGTAAT 
ACAGGGGCCT GGCGTGTGAG CACAATGAAT CATCCTCAGA TACAGGCTCC ATCGGTTATG 
AATGGCACTT CCCTTTCTCA TCTTAGCAAT GGAGAGCCAA AAAGTGGAGG CTCTTACGGT 
ACTACGTGGG GTGCCTATGG TTCTAATTAC TCTGGAGACA AATGTTCGAG CCCTAACGGC 
CAAGCTAATG GTGACACTGT GAATGCAACT CTAATGCAGC CTGGCATAAA TGGGCCTATG 
GGCACTAACT TTCAAGTGAA CACAAATAAA GGAGGAGGTG TGTGGGAGTC TGGGGCAACG 
AATTCCCAGA ATGCATCGTG GGGAAGTGGA AATGGCGCAA ATTCTGGAGG AAGTCGACGA 
GGATGGGGAA CTCCTGCACA AAACACTGGC ACTAATATAC CCAGCGTGGA GTGGAGCAAA 
CTGCCTAGCA ATCAGCATTC CAATGACAGT GCAAATGGCA ATGGTAAGAA GTTTACAAAT 
GGATGGAAAT CTACTGAGGA AGAGGATCAG GCTTCTGCCG CATCTCAGAC AACTGAGCAG 
AACAGTGTGT GGGCCAAAAC AGGAGGTACA GTGGAGAGTG AAGGTAGTAC AGAAAGCACT 
GGACGCCTCG AGGAGAAAGC CACTGGGGAA AATCAGAGTA GAGATCGGAG AAAAATTGAT 
CAGCACACAT TACTCCAAAG CATCGTAAAC AGAACTGACT TGGATCCACG TGTCCTCTCC 
AACTCTGGTT GGGGACAGAC TCCTATTAAG CAGAATACTG CCTGGGATAC GGAAACATCA 
CCTAGAGGGG AAAGAAAGAC TGACAATGGG ACAGAGGCCT GGGGAAGCTC TGCAACACAG 
ACTTTTAACT CAGGGGCATG TACAGATAAG ACTAGCCCTA CTAGTAATGA TACCTCATCT 
GTATCGGGGT GGGGAGATCC CAAACCTGCT CTGAGGTGGG GAGATTCCAA AGGCTCAAGC 
TGCCAGGGGG GATGGGAAGA TGATGCTGCT GCTACAGGAA TGGTCAAGAG CAATCAGTGG 
GGGAACAGCA AAGAAGAGAA GTCCGCATGG AATGATTCGC AAAAGAGCAA ACAGGGATGG 
GGTGATGGAC AGAAATCAAA CCAAGGGTGG TCCGTTTCTG CCAGTGATAA CTGGGGAGAA 
ACGTCAAGGA GTAACCATTG GGGTGAGGCT AATAAGAAAT CTAGCTCAGG AGGTAGTGAC 
AGTGACAGGT CTGTTTCTGG TTGGAATGAA CTTGGTAAAA CTAGTTCTTT TACTTGGGGA 
AATAACATAA ATCCAAATAA TTCGTCAGGA TGGGATGAAT CTTCTAAACC TAATCCTTCC 
CAGGGATGGG GAGATCCTCC AAAGTCTAAT CAGTCTCTAG GTTGGGGAGA TTCGTCAAAG 
CCAGTCACTT CTCCAGACTG GAACAAGCAA CAAGACATTG TCGGGTCTTG GGGAATCCCA 
CCAGCAACAG GCAAACCTCC TGGTACAGGC TGGCTGGGAG GACCTATACC AGCCCCAACA 
AAAGAAGAAG AGCCCACAGG CTGGGAGGAA CCATCCCCAG AATCCATACG TCGCAAAATG 
GAGATCGATG ACGGAACTTC AGCTTGGGGA GATCCGAGCA AATACAACTA CAAAAATGTG 
AACATGTGGA ACAAAAATGT CCCAAATGGC AGCAGCCGTT CAGACCAGCA AGCACAGGTA 
CACCAGTTGC TACCATCTGC AAGTGCCATC TCAAACAAAG AGGCAGGCAG TGGCTCTGGC 
TGGGGTGAGC CCTGGGGGGA GCCTTCTACT CCAGCCACAA CTGTGGATAA TGGTACTTCA 
GCATGGGGTA AACCCATAGA CAGTGGTCCC AGCTGGGGGG AGCCCATTGC TGCAGCATCC 
AACACATCCA CGTGGGGCTC CAGCTCTGTT GGTCCTCAAG CATTAAGCAA ATCTGGGCCA 
AAATCTATGC AAGATGGCTG GTGTGGTGAT GATATGCCAT TGCCTGGAAA TCGCCCCACT 
GGCTGGGAAG AGGAAGAGGA TGTAGAGATT GGAATGTGGA ATAGTAATTC ATCTCAAGAG 
CTTAACTCAT CTTTAAATTG GCCTCCATAT ACAAAGAAAA TGTCATCGAA GGGAACCATG 
AAAGGTGGAA ACAAACAAGA AGAAGCATGG ATAAATCCAT TCGTTAAACA GTTTTCAAAT 
ATCAGTTTTT CGAGAGACTC ACCCGAAGAA AATGTACAGA GCAATAAGAT GGATCTTTCC 
GGAGGAATGT TACAGGACAA ACGAATGGAG ATAGATAAAC ATAGCCTAAA TATTGGTGAT 
TACAATCGAA CGGTCGGGAA AGGCCCTGGT TCTCGGCCTC AGATTTCCAA AGAGTCTTCC 
ATGGAGCGCA GCCCTTACTT TGATAAGGAT GGCATTGTAG CAGATGAATC CCAAAACATG 
CAGTTTATGT CCAGTCAAAG CATGAAGCTT CCCCCTTCAA ATAGTGCACT ACCTAACCAG 
GCCCTTGGCT CCATAGCAGG GCTGGGTATG CAAAACTTGA ATTCTGTTAG ACAGAATGGC 
AATCCCAGTA TGTTTGGTGT TGGAAACACA GCAGCACAAC CCCGGGGCAT GCAGCAGCCT 
CCAGCACAAC CTCTTAGTTC ATCTCAGCCT AATCTTCGTG CTCAAGTGCC TCCTCCATTA 
CTCTCCCCTC AGGTTCCAGT TTCATTGCTG AAGTATGCAC CAAACAACGG TGGCCTGAAT 
CCGCTCTTTG GCCCTCAACA GGTAGCCATG CTGAACCAGC TATCCCAACT AAACCAGCTT 
TCTCAGATCT CCCAGTTACA GAGATTGTTA GCGCAGCAGC AAAGGGCGCA GAGTCAGAGA 
AGCGTGCCTT CTGGGAACCG GCAGCAGCAA GACCAGCAGG GTCGACCTCT TAGCGTGCAG 
CAGCAGATGA TGCAGCAGTC CCGGCAACTC GATCCCAGCC TGTTGGTGAA GCAGCAGACC 
CCGCCATCTC AGCAGCAGCC ACTCCATCAG CCAGCCATGA AATCCTTCCT CGAGAATGTC 
ATGCCCCACA CTACACCTGA GCTGCAGAAA GGGCCATCAC CAATAAATGC TTTCAGCAAC 
TTCCCGATAG GCTTGAACTC AAACTTGAAT GTAAATATGG ATATGAACAG TATTAAAGAG 
CCACAGTCAA GACTAAGGAA GTGGACTACA GTGGACAGCA TTTCTGTGAA CACATCTTTG 
GATCAAAACT CCAGCAAACA TGGTGCTATT TCAAGTGGTT TCAGGCTGGA AGAGTCTCCA 
TTTGTTCCCT ATGACTTTAT GAACAGCAGT ACTTCTCCAG CCAGCCCTCC GGGTTCAATA 
GGAGATGGCT GGCCACGTGC CAAATCGCCT AACGGCTCTA GCAGTGTTAA CTGGCCACCA 
GAATTTCGCC CTGGTGAACC ATGGAAAGGT TATCCAAACA TTGACCCTGA AACTGACCCT 
TACGTCACTC CTGGCAGTGT CATAAACAAT CTTTCAATTA ATACTGTGCG GGAAGTTGAC 
CACCTCAGGG ACAGGAACAG TGGGTCATCC TCATCCTTGA ACACCACGCT GCCTTCAACT 
AGTGCCTGGT CATCCATTCG TGCCTCCAAC TACAACGTTC CCCTCAGCAG TACAGCACAA 
AGCACTTCAG CCAGAAATAG TGATTCCAAA TTGACATGGT CTCCTGGTTC AGTTACAAAC 
ACCTCTCTGG CTCATGAGCT GTGGAAGGTC CCTTTACCAC CTAAAAACAT CACTGCTCCG 
TCCCGCCCAC CTCCGGGACT CACTGGTCAG AAGCCACCCT TGTCTACGTG GGATAACTCT 
CCCCTTCGTG TAGGTGGAGG ATGGGGAAAT TCTGACGCCA GGTACACCCC AGGTTCCAGT 
TGGGGTGAGA GCAGCTCAGG GAGAATAACA AATTGGCTTG TTCTAAAGAA CCTCACACCT 
CAGATTGACG GCTCAACTCT GCGTACTCTG TGCATGCAGC ATGGCCCACT GATAACATTC 
CACCTGAACC TCCCACATGG AAATGCTTTG GTCCGTTACA GTTCAAAAGA AGAGGTAGTG 
AAGGCACAAA AGTCTCTGCA CATGTGTGTA TTGGGGAACA CTACTATTCT TGCTGAGTTT 
GCCAGTGAAG AGGAGATCAG CCGTTTCTTT GCACAAAGTC AGTCTCTGAC CCCTTCTCCC 
GGCTGGCAGT CTCTCGGGTC CAGCCAGAGC CGGCTGGGCT CCCTCGACTG TTCCCACTCG 
TTCTCCAGCC GGACCGATCT CAATCACTGG AATGGTGCTG GGCTGTCGGG AACTAACTGT 
GGAGACCTTC ACGGCACTTC ACTCTGGGGG ACCCCACATT ATTCCACAAG CCTGTGGGGT 
CCTCCCAGCA GCAGCGACCC CCGGGGAATT AGCAGCCCAT CCCCCATTAA TGCTTTTCTT 
TCTGTTGACC ACCTGGGTGG GGGTGGAGAG TCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022275644.1
CDS	276..6194
Translation	MQLVAEPGLEARCPGSQDIPLPEEWNRELEAKATKDVERTLSRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNPQQQQQPQPPQQPQQPPQQPPQPPQQQPQPAQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLSSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSPSDSSTNCKNAVVNDSPETEAWPSVPGSDPELASECMDADSASSSESERNITIMASGSTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSPGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLDNNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVGGSSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVMNGTSLSHLSNGEPKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGINGPMGTNFQVNTNKGGGVWESGATNSQNASWGSGNGANSGGSRRGWGTPAQNTGTNIPSVEWSKLPSNQHSNDSANGNGKKFTNGWKSTEEEDQASAASQTTEQNSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACTDKTSPTSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDAAATGMVKSNQWGNSKEEKSAWNDSQKSKQGWGDGQKSNQGWSVSASDNWGETSRSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVTSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPTKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSASAISNKEAGSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASNTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGTMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERSPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPSLLVKQQTPPSQQQPLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_022419936.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022419936.1

ORF Insert Sequence:

ATGCAACTAG TGGCAGAGCC AGGCCTAGAA GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG AGAATTGGAA GCTAAAGCTA CCAAAGACGT AGAAAGGACT 
CTTAGCAGGG ATTTAGTGCA AGAAGAAGAA CAGTTGATGG AAGAAAAGAA AAAGAAAAAA 
GACGACAAGA AAAAGAAGGA AGCTGCTCAA AAGAAGGCCA CTGAACAAAA AATCAAAGTG 
CCAGAACAGA TAAAGCCCAG TGTAAGCCAG CCTCAGCCTG CCAACTCTAA TAACGGCACT 
TCCACAGCAA CCAGCACTAA TAATAATGCC AAGCGAGCCA CAGCCAACAA TCCGCAGCAG 
CAGCAGCAGC CACAGCCGCC ACAGCAGCCA CAGCAGCCGC CGCAGCAGCC ACCGCAGCCG 
CCACAGCAGC AGCCGCAGCC AGCACAGGCC TTGCCTCGGT ATCCTCGGGA AGTACCACCA 
CGATTTCGCC ACCAGGAACA CAAACAGCTT CTAAAGAGGG GTCAGCATTT TCCTGTCATA 
GCAGCAAACC TGGGATCTGC TGTTAAAGTG TTAAGCAGCC AGTCAGAAAG CAGTGCTTTA 
ACAAATCAAC AGCCACAAAA TAACGGAGAG GTGCAGAACA GCAAAAACCA GTCAGATATA 
AACCACAGTA CTTCAGGATC CCATTATGAA AATTCACAGC GGGGACCTGT GTCTTCTCCG 
AGTGACTCTA GCACAAATTG TAAGAATGCT GTTGTAAACG ACTCCCCTGA GACAGAAGCA 
TGGCCCTCAG TCCCTGGCAG TGATCCAGAG TTGGCTTCAG AATGTATGGA TGCTGATTCT 
GCCTCCAGTT CTGAATCAGA GAGAAACATC ACTATCATGG CTTCAGGGAG CACAGGTGGT 
GAAAAAGATG GCCTTCGGAA TAGCACTGGA CTTGGTTCCC AAAACAAGTT TGTGGTTGGT 
AGCAGCAGCA ATAATGTGGG CCATGGAAGT AGTCCTGGGC CATGGGGTTT TTCCCATGGA 
GCCATAATAA GCACATGTCA GGTCTCTGTG GATGCTCCTG AAAGCAAATC AGAAAGTAGC 
AACAATAGAA TGAATGCTTG GGGCACTGTA AGTTCTTCAT CAAATGGAGG GTTAAATCCA 
AGCACTTTGA ATTCAGCTAG CAACCATGGT GCCTGGCCAG TATTAGACAA CAACGGACTT 
GCCCTCAAAG GGCCTGTAGG GAGCGGTAGT TCTGGCATCA ATATTCAGTG TAGTACCATA 
GGCCAGATGC CTAACAATCA GAGTATTAAC TCTAAAGTGG GTGGTTCTTC CACCCATGGT 
ACCTGGGGAA GCCTTCAGGA AACTTGTGAA TCTGAAGTAA GTGGTACACA GAAGGTTTCA 
TTCAGTGGTC AACCTCAGAA TATTACCACT GAGATGACTG GACCAAATAA CACTACTAAC 
TTTATGACCT CTAGTTTACC AAACTCCGGT TCAGTACAGA ATAACGAACT GCCTAGTAAT 
ACAGGGGCCT GGCGTGTGAG CACAATGAAT CATCCTCAGA TACAGGCTCC ATCGGTTATG 
AATGGCACTT CCCTTTCTCA TCTTAGCAAT GGAGAGCCAA AAAGTGGAGG CTCTTACGGT 
ACTACGTGGG GTGCCTATGG TTCTAATTAC TCTGGAGACA AATGTTCGAG CCCTAACGGC 
CAAGCTAATG GTGACACTGT GAATGCAACT CTAATGCAGC CTGGCATAAA TGGGCCTATG 
GGCACTAACT TTCAAGTGAA CACAAATAAA GGAGGAGGTG TGTGGGAGTC TGGGGCAACG 
AATTCCCAGA ATGCATCGTG GGGAAGTGGA AATGGCGCAA ATTCTGGAGG AAGTCGACGA 
GGATGGGGAA CTCCTGCACA AAACACTGGC ACTAATATAC CCAGCGTGGA GTGGAGCAAA 
CTGCCTAGCA ATCAGCATTC CAATGACAGT GCAAATGGCA ATGGTAAGAA GTTTACAAAT 
GGATGGAAAT CTACTGAGGA AGAGGATCAG GCTTCTGCCG CATCTCAGAC AACTGAGCAG 
AACAGTGTGT GGGCCAAAAC AGGAGGTACA GTGGAGAGTG AAGGTAGTAC AGAAAGCACT 
GGACGCCTCG AGGAGAAAGC CACTGGGGAA AATCAGAGTA GAGATCGGAG AAAAATTGAT 
CAGCACACAT TACTCCAAAG CATCGTAAAC AGAACTGACT TGGATCCACG TGTCCTCTCC 
AACTCTGGTT GGGGACAGAC TCCTATTAAG CAGAATACTG CCTGGGATAC GGAAACATCA 
CCTAGAGGGG AAAGAAAGAC TGACAATGGG ACAGAGGCCT GGGGAAGCTC TGCAACACAG 
ACTTTTAACT CAGGGGCATG TACAGATAAG ACTAGCCCTA CTAGTAATGA TACCTCATCT 
GTATCGGGGT GGGGAGATCC CAAACCTGCT CTGAGGTGGG GAGATTCCAA AGGCTCAAGC 
TGCCAGGGGG GATGGGAAGA TGATGCTGCT GCTACAGGAA TGGTCAAGAG CAATCAGTGG 
GGGAACAGCA AAGAAGAGAA GTCCGCATGG AATGATTCGC AAAAGAGCAA ACAGGGATGG 
GGTGATGGAC AGAAATCAAA CCAAGGGTGG TCCGTTTCTG CCAGTGATAA CTGGGGAGAA 
ACGTCAAGGA GTAACCATTG GGGTGAGGCT AATAAGAAAT CTAGCTCAGG AGGTAGTGAC 
AGTGACAGGT CTGTTTCTGG TTGGAATGAA CTTGGTAAAA CTAGTTCTTT TACTTGGGGA 
AATAACATAA ATCCAAATAA TTCGTCAGGA TGGGATGAAT CTTCTAAACC TAATCCTTCC 
CAGGGATGGG GAGATCCTCC AAAGTCTAAT CAGTCTCTAG GTTGGGGAGA TTCGTCAAAG 
CCAGTCACTT CTCCAGACTG GAACAAGCAA CAAGACATTG TCGGGTCTTG GGGAATCCCA 
CCAGCAACAG GCAAACCTCC TGGTACAGGC TGGCTGGGAG GACCTATACC AGCCCCAACA 
AAAGAAGAAG AGCCCACAGG CTGGGAGGAA CCATCCCCAG AATCCATACG TCGCAAAATG 
GAGATCGATG ACGGAACTTC AGCTTGGGGA GATCCGAGCA AATACAACTA CAAAAATGTG 
AACATGTGGA ACAAAAATGT CCCAAATGGC AGCAGCCGTT CAGACCAGCA AGCACAGGTA 
CACCAGTTGC TACCATCTGC AAGTGCCATC TCAAACAAAG AGGCAGGCAG TGGCTCTGGC 
TGGGGTGAGC CCTGGGGGGA GCCTTCTACT CCAGCCACAA CTGTGGATAA TGGTACTTCA 
GCATGGGGTA AACCCATAGA CAGTGGTCCC AGCTGGGGGG AGCCCATTGC TGCAGCATCC 
AACACATCCA CGTGGGGCTC CAGCTCTGTT GGTCCTCAAG CATTAAGCAA ATCTGGGCCA 
AAATCTATGC AAGATGGCTG GTGTGGTGAT GATATGCCAT TGCCTGGAAA TCGCCCCACT 
GGCTGGGAAG AGGAAGAGGA TGTAGAGATT GGAATGTGGA ATAGTAATTC ATCTCAAGAG 
CTTAACTCAT CTTTAAATTG GCCTCCATAT ACAAAGAAAA TGTCATCGAA GGGAACCATG 
AAAGGTGGAA ACAAACAAGA AGAAGCATGG ATAAATCCAT TCGTTAAACA GTTTTCAAAT 
ATCAGTTTTT CGAGAGACTC ACCCGAAGAA AATGTACAGA GCAATAAGAT GGATCTTTCC 
GGAGGAATGT TACAGGACAA ACGAATGGAG ATAGATAAAC ATAGCCTAAA TATTGGTGAT 
TACAATCGAA CGGTCGGGAA AGGCCCTGGT TCTCGGCCTC AGATTTCCAA AGAGTCTTCC 
ATGGAGCGCA GCCCTTACTT TGATAAGGAT GGCATTGTAG CAGATGAATC CCAAAACATG 
CAGTTTATGT CCAGTCAAAG CATGAAGCTT CCCCCTTCAA ATAGTGCACT ACCTAACCAG 
GCCCTTGGCT CCATAGCAGG GCTGGGTATG CAAAACTTGA ATTCTGTTAG ACAGAATGGC 
AATCCCAGTA TGTTTGGTGT TGGAAACACA GCAGCACAAC CCCGGGGCAT GCAGCAGCCT 
CCAGCACAAC CTCTTAGTTC ATCTCAGCCT AATCTTCGTG CTCAAGTGCC TCCTCCATTA 
CTCTCCCCTC AGGTTCCAGT TTCATTGCTG AAGTATGCAC CAAACAACGG TGGCCTGAAT 
CCGCTCTTTG GCCCTCAACA GGTAGCCATG CTGAACCAGC TATCCCAACT AAACCAGCTT 
TCTCAGATCT CCCAGTTACA GAGATTGTTA GCGCAGCAGC AAAGGGCGCA GAGTCAGAGA 
AGCGTGCCTT CTGGGAACCG GCAGCAGCAA GACCAGCAGG GTCGACCTCT TAGCGTGCAG 
CAGCAGATGA TGCAGCAGTC CCGGCAACTC GATCCCAGCC TGTTGGTGAA GCAGCAGACC 
CCGCCATCTC AGCAGCAGCC ACTCCATCAG CCAGCCATGA AATCCTTCCT CGAGAATGTC 
ATGCCCCACA CTACACCTGA GCTGCAGAAA GGGCCATCAC CAATAAATGC TTTCAGCAAC 
TTCCCGATAG GCTTGAACTC AAACTTGAAT GTAAATATGG ATATGAACAG TATTAAAGAG 
CCACAGTCAA GACTAAGGAA GTGGACTACA GTGGACAGCA TTTCTGTGAA CACATCTTTG 
GATCAAAACT CCAGCAAACA TGGTGCTATT TCAAGTGGTT TCAGGCTGGA AGAGTCTCCA 
TTTGTTCCCT ATGACTTTAT GAACAGCAGT ACTTCTCCAG CCAGCCCTCC GGGTTCAATA 
GGAGATGGCT GGCCACGTGC CAAATCGCCT AACGGCTCTA GCAGTGTTAA CTGGCCACCA 
GAATTTCGCC CTGGTGAACC ATGGAAAGGT TATCCAAACA TTGACCCTGA AACTGACCCT 
TACGTCACTC CTGGCAGTGT CATAAACAAT CTTTCAATTA ATACTGTGCG GGAAGTTGAC 
CACCTCAGGG ACAGGAACAG TGGGTCATCC TCATCCTTGA ACACCACGCT GCCTTCAACT 
AGTGCCTGGT CATCCATTCG TGCCTCCAAC TACAACGTTC CCCTCAGCAG TACAGCACAA 
AGCACTTCAG CCAGAAATAG TGATTCCAAA TTGACATGGT CTCCTGGTTC AGTTACAAAC 
ACCTCTCTGG CTCATGAGCT GTGGAAGGTC CCTTTACCAC CTAAAAACAT CACTGCTCCG 
TCCCGCCCAC CTCCGGGACT CACTGGTCAG AAGCCACCCT TGTCTACGTG GGATAACTCT 
CCCCTTCGTG TAGGTGGAGG ATGGGGAAAT TCTGACGCCA GGTACACCCC AGGTTCCAGT 
TGGGGTGAGA GCAGCTCAGG GAGAATAACA AATTGGCTTG TTCTAAAGAA CCTCACACCT 
CAGATTGACG GCTCAACTCT GCGTACTCTG TGCATGCAGC ATGGCCCACT GATAACATTC 
CACCTGAACC TCCCACATGG AAATGCTTTG GTCCGTTACA GTTCAAAAGA AGAGGTAGTG 
AAGGCACAAA AGTCTCTGCA CATGTGTGTA TTGGGGAACA CTACTATTCT TGCTGAGTTT 
GCCAGTGAAG AGGAGATCAG CCGTTTCTTT GCACAAAGTC AGTCTCTGAC CCCTTCTCCC 
GGCTGGCAGT CTCTCGGGTC CAGCCAGAGC CGGCTGGGCT CCCTCGACTG TTCCCACTCG 
TTCTCCAGCC GGACCGATCT CAATCACTGG AATGGTGCTG GGCTGTCGGG AACTAACTGT 
GGAGACCTTC ACGGCACTTC ACTCTGGGGG ACCCCACATT ATTCCACAAG CCTGTGGGGT 
CCTCCCAGCA GCAGCGACCC CCGGGGAATT AGCAGCCCAT CCCCCATTAA TGCTTTTCTT 
TCTGTTGACC ACCTGGGTGG GGGTGGAGAG TCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_005621401.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005621458.1 Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X4, mRNA.

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CloneID	OCe07958
Clone ID Related Accession (Same CDS sequence)	XM_005621401.3 , XM_005621401.2
Accession Version	XM_005621401.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	trinucleotide repeat-containing gene 6A protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005621401.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACTAG TGGCAGAGCC AGGCCTAGAA GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG AGAATTGGAA GCTAAAGCTA CCAAAGACGT AGAAAGGACT 
CTTAGCAGGG ATTTAGTGCA AGAAGAAGAA CAGTTGATGG AAGAAAAGAA AAAGAAAAAA 
GACGACAAGA AAAAGAAGGA AGCTGCTCAA AAGAAGGCCA CTGAACAAAA AATCAAAGTG 
CCAGAACAGA TAAAGCCCAG TGTAAGCCAG CCTCAGCCTG CCAACTCTAA TAACGGCACT 
TCCACAGCAA CCAGCACTAA TAATAATGCC AAGCGAGCCA CAGCCAACAA TCCGCAGCAG 
CAGCAGCAGC CACAGCCGCC ACAGCAGCCA CAGCAGCCGC CGCAGCAGCC ACCGCAGCCG 
CCACAGCAGC AGCCGCAGCC AGCACAGGCC TTGCCTCGGT ATCCTCGGGA AGTACCACCA 
CGATTTCGCC ACCAGGAACA CAAACAGCTT CTAAAGAGGG GTCAGCATTT TCCTGTCATA 
GCAGCAAACC TGGGATCTGC TGTTAAAGTG TTAAGCAGCC AGTCAGAAAG CAGTGCTTTA 
ACAAATCAAC AGCCACAAAA TAACGGAGAG GTGCAGAACA GCAAAAACCA GTCAGATATA 
AACCACAGTA CTTCAGGATC CCATTATGAA AATTCACAGC GGGGACCTGT GTCTTCTCCG 
AGTGACTCTA GCACAAATTG TAAGAATGCT GTTGTAAACG ACTCCCCTGA GACAGAAGCA 
TGGCCCTCAG TCCCTGGCAG TGATCCAGAG TTGGCTTCAG AATGTATGGA TGCTGATTCT 
GCCTCCAGTT CTGAATCAGA GAGAAACATC ACTATCATGG CTTCAGGGAG CACAGGTGGT 
GAAAAAGATG GCCTTCGGAA TAGCACTGGA CTTGGTTCCC AAAACAAGTT TGTGGTTGGT 
AGCAGCAGCA ATAATGTGGG CCATGGAAGT AGTCCTGGGC CATGGGGTTT TTCCCATGGA 
GCCATAATAA GCACATGTCA GGTCTCTGTG GATGCTCCTG AAAGCAAATC AGAAAGTAGC 
AACAATAGAA TGAATGCTTG GGGCACTGTA AGTTCTTCAT CAAATGGAGG GTTAAATCCA 
AGCACTTTGA ATTCAGCTAG CAACCATGGT GCCTGGCCAG TATTAGACAA CAACGGACTT 
GCCCTCAAAG GGCCTGTAGG GAGCGGTAGT TCTGGCATCA ATATTCAGTG TAGTACCATA 
GGCCAGATGC CTAACAATCA GAGTATTAAC TCTAAAGTGG GTGGTTCTTC CACCCATGGT 
ACCTGGGGAA GCCTTCAGGA AACTTGTGAA TCTGAAGTAA GTGGTACACA GAAGGTTTCA 
TTCAGTGGTC AACCTCAGAA TATTACCACT GAGATGACTG GACCAAATAA CACTACTAAC 
TTTATGACCT CTAGTTTACC AAACTCCGGT TCAGTACAGA ATAACGAACT GCCTAGTAAT 
ACAGGGGCCT GGCGTGTGAG CACAATGAAT CATCCTCAGA TACAGGCTCC ATCGGTTATG 
AATGGCACTT CCCTTTCTCA TCTTAGCAAT GGAGAGCCAA AAAGTGGAGG CTCTTACGGT 
ACTACGTGGG GTGCCTATGG TTCTAATTAC TCTGGAGACA AATGTTCGAG CCCTAACGGC 
CAAGCTAATG GTGACACTGT GAATGCAACT CTAATGCAGC CTGGCATAAA TGGGCCTATG 
GGCACTAACT TTCAAGTGAA CACAAATAAA GGAGGAGGTG TGTGGGAGTC TGGGGCAACG 
AATTCCCAGA ATGCATCGTG GGGAAGTGGA AATGGCGCAA ATTCTGGAGG AAGTCGACGA 
GGATGGGGAA CTCCTGCACA AAACACTGGC ACTAATATAC CCAGCGTGGA GTGGAGCAAA 
CTGCCTAGCA ATCAGCATTC CAATGACAGT GCAAATGGCA ATGGTAAGAA GTTTACAAAT 
GGATGGAAAT CTACTGAGGA AGAGGATCAG GCTTCTGCCG CATCTCAGAC AACTGAGCAG 
AACAGTGTGT GGGCCAAAAC AGGAGGTACA GTGGAGAGTG AAGGTAGTAC AGAAAGCACT 
GGACGCCTCG AGGAGAAAGC CACTGGGGAA AATCAGAGTA GAGATCGGAG AAAAATTGAT 
CAGCACACAT TACTCCAAAG CATCGTAAAC AGAACTGACT TGGATCCACG TGTCCTCTCC 
AACTCTGGTT GGGGACAGAC TCCTATTAAG CAGAATACTG CCTGGGATAC GGAAACATCA 
CCTAGAGGGG AAAGAAAGAC TGACAATGGG ACAGAGGCCT GGGGAAGCTC TGCAACACAG 
ACTTTTAACT CAGGGGCATG TACAGATAAG ACTAGCCCTA CTAGTAATGA TACCTCATCT 
GTATCGGGGT GGGGAGATCC CAAACCTGCT CTGAGGTGGG GAGATTCCAA AGGCTCAAGC 
TGCCAGGGGG GATGGGAAGA TGATGCTGCT GCTACAGGAA TGGTCAAGAG CAATCAGTGG 
GGGAACAGCA AAGAAGAGAA GTCCGCATGG AATGATTCGC AAAAGAGCAA ACAGGGATGG 
GGTGATGGAC AGAAATCAAA CCAAGGGTGG TCCGTTTCTG CCAGTGATAA CTGGGGAGAA 
ACGTCAAGGA GTAACCATTG GGGTGAGGCT AATAAGAAAT CTAGCTCAGG AGGTAGTGAC 
AGTGACAGGT CTGTTTCTGG TTGGAATGAA CTTGGTAAAA CTAGTTCTTT TACTTGGGGA 
AATAACATAA ATCCAAATAA TTCGTCAGGA TGGGATGAAT CTTCTAAACC TAATCCTTCC 
CAGGGATGGG GAGATCCTCC AAAGTCTAAT CAGTCTCTAG GTTGGGGAGA TTCGTCAAAG 
CCAGTCACTT CTCCAGACTG GAACAAGCAA CAAGACATTG TCGGGTCTTG GGGAATCCCA 
CCAGCAACAG GCAAACCTCC TGGTACAGGC TGGCTGGGAG GACCTATACC AGCCCCAACA 
AAAGAAGAAG AGCCCACAGG CTGGGAGGAA CCATCCCCAG AATCCATACG TCGCAAAATG 
GAGATCGATG ACGGAACTTC AGCTTGGGGA GATCCGAGCA AATACAACTA CAAAAATGTG 
AACATGTGGA ACAAAAATGT CCCAAATGGC AGCAGCCGTT CAGACCAGCA AGCACAGGTA 
CACCAGTTGC TACCATCTGC AAGTGCCATC TCAAACAAAG AGGCAGGCAG TGGCTCTGGC 
TGGGGTGAGC CCTGGGGGGA GCCTTCTACT CCAGCCACAA CTGTGGATAA TGGTACTTCA 
GCATGGGGTA AACCCATAGA CAGTGGTCCC AGCTGGGGGG AGCCCATTGC TGCAGCATCC 
AACACATCCA CGTGGGGCTC CAGCTCTGTT GGTCCTCAAG CATTAAGCAA ATCTGGGCCA 
AAATCTATGC AAGATGGCTG GTGTGGTGAT GATATGCCAT TGCCTGGAAA TCGCCCCACT 
GGCTGGGAAG AGGAAGAGGA TGTAGAGATT GGAATGTGGA ATAGTAATTC ATCTCAAGAG 
CTTAACTCAT CTTTAAATTG GCCTCCATAT ACAAAGAAAA TGTCATCGAA GGGTCTGAGT 
GGCAAAAAAA GGAGAAGGGA AAGGGGAACC ATGAAAGGTG GAAACAAACA AGAAGAAGCA 
TGGATAAATC CATTCGTTAA ACAGTTTTCA AATATCAGTT TTTCGAGAGA CTCACCCGAA 
GAAAATGTAC AGAGCAATAA GATGGATCTT TCCGGAGGAA TGTTACAGGA CAAACGAATG 
GAGATAGATA AACATAGCCT AAATATTGGT GATTACAATC GAACGGTCGG GAAAGGCCCT 
GGTTCTCGGC CTCAGATTTC CAAAGAGTCT TCCATGGAGC GCAGCCCTTA CTTTGATAAG 
GATGGCATTG TAGCAGATGA ATCCCAAAAC ATGCAGTTTA TGTCCAGTCA AAGCATGAAG 
CTTCCCCCTT CAAATAGTGC ACTACCTAAC CAGGCCCTTG GCTCCATAGC AGGGCTGGGT 
ATGCAAAACT TGAATTCTGT TAGACAGAAT GGCAATCCCA GTATGTTTGG TGTTGGAAAC 
ACAGCAGCAC AACCCCGGGG CATGCAGCAG CCTCCAGCAC AACCTCTTAG TTCATCTCAG 
CCTAATCTTC GTGCTCAAGT GCCTCCTCCA TTACTCTCCC CTCAGGTAGC CATGCTGAAC 
CAGCTATCCC AACTAAACCA GCTTTCTCAG ATCTCCCAGT TACAGAGATT GTTAGCGCAG 
CAGCAAAGGG CGCAGAGTCA GAGAAGCGTG CCTTCTGGGA ACCGGCAGCA GCAAGACCAG 
CAGGGTCGAC CTCTTAGCGT GCAGCAGCAG ATGATGCAGC AGTCCCGGCA ACTCGATCCC 
AGCCTGTTGG TGAAGCAGCA GACCCCGCCA TCTCAGCAGC AGCCACTCCA TCAGCCAGCC 
ATGAAATCCT TCCTCGAGAA TGTCATGCCC CACACTACAC CTGAGCTGCA GAAAGGGCCA 
TCACCAATAA ATGCTTTCAG CAACTTCCCG ATAGGCTTGA ACTCAAACTT GAATGTAAAT 
ATGGATATGA ACAGTATTAA AGAGCCACAG TCAAGACTAA GGAAGTGGAC TACAGTGGAC 
AGCATTTCTG TGAACACATC TTTGGATCAA AACTCCAGCA AACATGGTGC TATTTCAAGT 
GGTTTCAGGC TGGAAGAGTC TCCATTTGTT CCCTATGACT TTATGAACAG CAGTACTTCT 
CCAGCCAGCC CTCCGGGTTC AATAGGAGAT GGCTGGCCAC GTGCCAAATC GCCTAACGGC 
TCTAGCAGTG TTAACTGGCC ACCAGAATTT CGCCCTGGTG AACCATGGAA AGGTTATCCA 
AACATTGACC CTGAAACTGA CCCTTACGTC ACTCCTGGCA GTGTCATAAA CAATCTTTCA 
ATTAATACTG TGCGGGAAGT TGACCACCTC AGGGACAGGA ACAGTGGGTC ATCCTCATCC 
TTGAACACCA CGCTGCCTTC AACTAGTGCC TGGTCATCCA TTCGTGCCTC CAACTACAAC 
GTTCCCCTCA GCAGTACAGC ACAAAGCACT TCAGCCAGAA ATAGTGATTC CAAATTGACA 
TGGTCTCCTG GTTCAGTTAC AAACACCTCT CTGGCTCATG AGCTGTGGAA GGTCCCTTTA 
CCACCTAAAA ACATCACTGC TCCGTCCCGC CCACCTCCGG GACTCACTGG TCAGAAGCCA 
CCCTTGTCTA CGTGGGATAA CTCTCCCCTT CGTGTAGGTG GAGGATGGGG AAATTCTGAC 
GCCAGGTACA CCCCAGGTTC CAGTTGGGGT GAGAGCAGCT CAGGGAGAAT AACAAATTGG 
CTTGTTCTAA AGAACCTCAC ACCTCAGATT GACGGCTCAA CTCTGCGTAC TCTGTGCATG 
CAGCATGGCC CACTGATAAC ATTCCACCTG AACCTCCCAC ATGGAAATGC TTTGGTCCGT 
TACAGTTCAA AAGAAGAGGT AGTGAAGGCA CAAAAGTCTC TGCACATGTG TGTATTGGGG 
AACACTACTA TTCTTGCTGA GTTTGCCAGT GAAGAGGAGA TCAGCCGTTT CTTTGCACAA 
AGTCAGTCTC TGACCCCTTC TCCCGGCTGG CAGTCTCTCG GGTCCAGCCA GAGCCGGCTG 
GGCTCCCTCG ACTGTTCCCA CTCGTTCTCC AGCCGGACCG ATCTCAATCA CTGGAATGGT 
GCTGGGCTGT CGGGAACTAA CTGTGGAGAC CTTCACGGCA CTTCACTCTG GGGGACCCCA 
CATTATTCCA CAAGCCTGTG GGGTCCTCCC AGCAGCAGCG ACCCCCGGGG AATTAGCAGC 
CCATCCCCCA TTAATGCTTT TCTTTCTGTT GACCACCTGG GTGGGGGTGG AGAGTCCATG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005621458.1
CDS	277..6159
Translation	MQLVAEPGLEARCPGSQDIPLPEEWNRELEAKATKDVERTLSRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNPQQQQQPQPPQQPQQPPQQPPQPPQQQPQPAQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLSSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSPSDSSTNCKNAVVNDSPETEAWPSVPGSDPELASECMDADSASSSESERNITIMASGSTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSPGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLDNNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVGGSSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVMNGTSLSHLSNGEPKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGINGPMGTNFQVNTNKGGGVWESGATNSQNASWGSGNGANSGGSRRGWGTPAQNTGTNIPSVEWSKLPSNQHSNDSANGNGKKFTNGWKSTEEEDQASAASQTTEQNSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACTDKTSPTSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDAAATGMVKSNQWGNSKEEKSAWNDSQKSKQGWGDGQKSNQGWSVSASDNWGETSRSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVTSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPTKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSASAISNKEAGSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASNTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGTMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERSPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPSLLVKQQTPPSQQQPLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_005621401.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005621401.3

ORF Insert Sequence:

ATGCAACTAG TGGCAGAGCC AGGCCTAGAA GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG AGAATTGGAA GCTAAAGCTA CCAAAGACGT AGAAAGGACT 
CTTAGCAGGG ATTTAGTGCA AGAAGAAGAA CAGTTGATGG AAGAAAAGAA AAAGAAAAAA 
GACGACAAGA AAAAGAAGGA AGCTGCTCAA AAGAAGGCCA CTGAACAAAA AATCAAAGTG 
CCAGAACAGA TAAAGCCCAG TGTAAGCCAG CCTCAGCCTG CCAACTCTAA TAACGGCACT 
TCCACAGCAA CCAGCACTAA TAATAATGCC AAGCGAGCCA CAGCCAACAA TCCGCAGCAG 
CAGCAGCAGC CACAGCCGCC ACAGCAGCCA CAGCAGCCGC CGCAGCAGCC ACCGCAGCCG 
CCACAGCAGC AGCCGCAGCC AGCACAGGCC TTGCCTCGGT ATCCTCGGGA AGTACCACCA 
CGATTTCGCC ACCAGGAACA CAAACAGCTT CTAAAGAGGG GTCAGCATTT TCCTGTCATA 
GCAGCAAACC TGGGATCTGC TGTTAAAGTG TTAAGCAGCC AGTCAGAAAG CAGTGCTTTA 
ACAAATCAAC AGCCACAAAA TAACGGAGAG GTGCAGAACA GCAAAAACCA GTCAGATATA 
AACCACAGTA CTTCAGGATC CCATTATGAA AATTCACAGC GGGGACCTGT GTCTTCTCCG 
AGTGACTCTA GCACAAATTG TAAGAATGCT GTTGTAAACG ACTCCCCTGA GACAGAAGCA 
TGGCCCTCAG TCCCTGGCAG TGATCCAGAG TTGGCTTCAG AATGTATGGA TGCTGATTCT 
GCCTCCAGTT CTGAATCAGA GAGAAACATC ACTATCATGG CTTCAGGGAG CACAGGTGGT 
GAAAAAGATG GCCTTCGGAA TAGCACTGGA CTTGGTTCCC AAAACAAGTT TGTGGTTGGT 
AGCAGCAGCA ATAATGTGGG CCATGGAAGT AGTCCTGGGC CATGGGGTTT TTCCCATGGA 
GCCATAATAA GCACATGTCA GGTCTCTGTG GATGCTCCTG AAAGCAAATC AGAAAGTAGC 
AACAATAGAA TGAATGCTTG GGGCACTGTA AGTTCTTCAT CAAATGGAGG GTTAAATCCA 
AGCACTTTGA ATTCAGCTAG CAACCATGGT GCCTGGCCAG TATTAGACAA CAACGGACTT 
GCCCTCAAAG GGCCTGTAGG GAGCGGTAGT TCTGGCATCA ATATTCAGTG TAGTACCATA 
GGCCAGATGC CTAACAATCA GAGTATTAAC TCTAAAGTGG GTGGTTCTTC CACCCATGGT 
ACCTGGGGAA GCCTTCAGGA AACTTGTGAA TCTGAAGTAA GTGGTACACA GAAGGTTTCA 
TTCAGTGGTC AACCTCAGAA TATTACCACT GAGATGACTG GACCAAATAA CACTACTAAC 
TTTATGACCT CTAGTTTACC AAACTCCGGT TCAGTACAGA ATAACGAACT GCCTAGTAAT 
ACAGGGGCCT GGCGTGTGAG CACAATGAAT CATCCTCAGA TACAGGCTCC ATCGGTTATG 
AATGGCACTT CCCTTTCTCA TCTTAGCAAT GGAGAGCCAA AAAGTGGAGG CTCTTACGGT 
ACTACGTGGG GTGCCTATGG TTCTAATTAC TCTGGAGACA AATGTTCGAG CCCTAACGGC 
CAAGCTAATG GTGACACTGT GAATGCAACT CTAATGCAGC CTGGCATAAA TGGGCCTATG 
GGCACTAACT TTCAAGTGAA CACAAATAAA GGAGGAGGTG TGTGGGAGTC TGGGGCAACG 
AATTCCCAGA ATGCATCGTG GGGAAGTGGA AATGGCGCAA ATTCTGGAGG AAGTCGACGA 
GGATGGGGAA CTCCTGCACA AAACACTGGC ACTAATATAC CCAGCGTGGA GTGGAGCAAA 
CTGCCTAGCA ATCAGCATTC CAATGACAGT GCAAATGGCA ATGGTAAGAA GTTTACAAAT 
GGATGGAAAT CTACTGAGGA AGAGGATCAG GCTTCTGCCG CATCTCAGAC AACTGAGCAG 
AACAGTGTGT GGGCCAAAAC AGGAGGTACA GTGGAGAGTG AAGGTAGTAC AGAAAGCACT 
GGACGCCTCG AGGAGAAAGC CACTGGGGAA AATCAGAGTA GAGATCGGAG AAAAATTGAT 
CAGCACACAT TACTCCAAAG CATCGTAAAC AGAACTGACT TGGATCCACG TGTCCTCTCC 
AACTCTGGTT GGGGACAGAC TCCTATTAAG CAGAATACTG CCTGGGATAC GGAAACATCA 
CCTAGAGGGG AAAGAAAGAC TGACAATGGG ACAGAGGCCT GGGGAAGCTC TGCAACACAG 
ACTTTTAACT CAGGGGCATG TACAGATAAG ACTAGCCCTA CTAGTAATGA TACCTCATCT 
GTATCGGGGT GGGGAGATCC CAAACCTGCT CTGAGGTGGG GAGATTCCAA AGGCTCAAGC 
TGCCAGGGGG GATGGGAAGA TGATGCTGCT GCTACAGGAA TGGTCAAGAG CAATCAGTGG 
GGGAACAGCA AAGAAGAGAA GTCCGCATGG AATGATTCGC AAAAGAGCAA ACAGGGATGG 
GGTGATGGAC AGAAATCAAA CCAAGGGTGG TCCGTTTCTG CCAGTGATAA CTGGGGAGAA 
ACGTCAAGGA GTAACCATTG GGGTGAGGCT AATAAGAAAT CTAGCTCAGG AGGTAGTGAC 
AGTGACAGGT CTGTTTCTGG TTGGAATGAA CTTGGTAAAA CTAGTTCTTT TACTTGGGGA 
AATAACATAA ATCCAAATAA TTCGTCAGGA TGGGATGAAT CTTCTAAACC TAATCCTTCC 
CAGGGATGGG GAGATCCTCC AAAGTCTAAT CAGTCTCTAG GTTGGGGAGA TTCGTCAAAG 
CCAGTCACTT CTCCAGACTG GAACAAGCAA CAAGACATTG TCGGGTCTTG GGGAATCCCA 
CCAGCAACAG GCAAACCTCC TGGTACAGGC TGGCTGGGAG GACCTATACC AGCCCCAACA 
AAAGAAGAAG AGCCCACAGG CTGGGAGGAA CCATCCCCAG AATCCATACG TCGCAAAATG 
GAGATCGATG ACGGAACTTC AGCTTGGGGA GATCCGAGCA AATACAACTA CAAAAATGTG 
AACATGTGGA ACAAAAATGT CCCAAATGGC AGCAGCCGTT CAGACCAGCA AGCACAGGTA 
CACCAGTTGC TACCATCTGC AAGTGCCATC TCAAACAAAG AGGCAGGCAG TGGCTCTGGC 
TGGGGTGAGC CCTGGGGGGA GCCTTCTACT CCAGCCACAA CTGTGGATAA TGGTACTTCA 
GCATGGGGTA AACCCATAGA CAGTGGTCCC AGCTGGGGGG AGCCCATTGC TGCAGCATCC 
AACACATCCA CGTGGGGCTC CAGCTCTGTT GGTCCTCAAG CATTAAGCAA ATCTGGGCCA 
AAATCTATGC AAGATGGCTG GTGTGGTGAT GATATGCCAT TGCCTGGAAA TCGCCCCACT 
GGCTGGGAAG AGGAAGAGGA TGTAGAGATT GGAATGTGGA ATAGTAATTC ATCTCAAGAG 
CTTAACTCAT CTTTAAATTG GCCTCCATAT ACAAAGAAAA TGTCATCGAA GGGTCTGAGT 
GGCAAAAAAA GGAGAAGGGA AAGGGGAACC ATGAAAGGTG GAAACAAACA AGAAGAAGCA 
TGGATAAATC CATTCGTTAA ACAGTTTTCA AATATCAGTT TTTCGAGAGA CTCACCCGAA 
GAAAATGTAC AGAGCAATAA GATGGATCTT TCCGGAGGAA TGTTACAGGA CAAACGAATG 
GAGATAGATA AACATAGCCT AAATATTGGT GATTACAATC GAACGGTCGG GAAAGGCCCT 
GGTTCTCGGC CTCAGATTTC CAAAGAGTCT TCCATGGAGC GCAGCCCTTA CTTTGATAAG 
GATGGCATTG TAGCAGATGA ATCCCAAAAC ATGCAGTTTA TGTCCAGTCA AAGCATGAAG 
CTTCCCCCTT CAAATAGTGC ACTACCTAAC CAGGCCCTTG GCTCCATAGC AGGGCTGGGT 
ATGCAAAACT TGAATTCTGT TAGACAGAAT GGCAATCCCA GTATGTTTGG TGTTGGAAAC 
ACAGCAGCAC AACCCCGGGG CATGCAGCAG CCTCCAGCAC AACCTCTTAG TTCATCTCAG 
CCTAATCTTC GTGCTCAAGT GCCTCCTCCA TTACTCTCCC CTCAGGTAGC CATGCTGAAC 
CAGCTATCCC AACTAAACCA GCTTTCTCAG ATCTCCCAGT TACAGAGATT GTTAGCGCAG 
CAGCAAAGGG CGCAGAGTCA GAGAAGCGTG CCTTCTGGGA ACCGGCAGCA GCAAGACCAG 
CAGGGTCGAC CTCTTAGCGT GCAGCAGCAG ATGATGCAGC AGTCCCGGCA ACTCGATCCC 
AGCCTGTTGG TGAAGCAGCA GACCCCGCCA TCTCAGCAGC AGCCACTCCA TCAGCCAGCC 
ATGAAATCCT TCCTCGAGAA TGTCATGCCC CACACTACAC CTGAGCTGCA GAAAGGGCCA 
TCACCAATAA ATGCTTTCAG CAACTTCCCG ATAGGCTTGA ACTCAAACTT GAATGTAAAT 
ATGGATATGA ACAGTATTAA AGAGCCACAG TCAAGACTAA GGAAGTGGAC TACAGTGGAC 
AGCATTTCTG TGAACACATC TTTGGATCAA AACTCCAGCA AACATGGTGC TATTTCAAGT 
GGTTTCAGGC TGGAAGAGTC TCCATTTGTT CCCTATGACT TTATGAACAG CAGTACTTCT 
CCAGCCAGCC CTCCGGGTTC AATAGGAGAT GGCTGGCCAC GTGCCAAATC GCCTAACGGC 
TCTAGCAGTG TTAACTGGCC ACCAGAATTT CGCCCTGGTG AACCATGGAA AGGTTATCCA 
AACATTGACC CTGAAACTGA CCCTTACGTC ACTCCTGGCA GTGTCATAAA CAATCTTTCA 
ATTAATACTG TGCGGGAAGT TGACCACCTC AGGGACAGGA ACAGTGGGTC ATCCTCATCC 
TTGAACACCA CGCTGCCTTC AACTAGTGCC TGGTCATCCA TTCGTGCCTC CAACTACAAC 
GTTCCCCTCA GCAGTACAGC ACAAAGCACT TCAGCCAGAA ATAGTGATTC CAAATTGACA 
TGGTCTCCTG GTTCAGTTAC AAACACCTCT CTGGCTCATG AGCTGTGGAA GGTCCCTTTA 
CCACCTAAAA ACATCACTGC TCCGTCCCGC CCACCTCCGG GACTCACTGG TCAGAAGCCA 
CCCTTGTCTA CGTGGGATAA CTCTCCCCTT CGTGTAGGTG GAGGATGGGG AAATTCTGAC 
GCCAGGTACA CCCCAGGTTC CAGTTGGGGT GAGAGCAGCT CAGGGAGAAT AACAAATTGG 
CTTGTTCTAA AGAACCTCAC ACCTCAGATT GACGGCTCAA CTCTGCGTAC TCTGTGCATG 
CAGCATGGCC CACTGATAAC ATTCCACCTG AACCTCCCAC ATGGAAATGC TTTGGTCCGT 
TACAGTTCAA AAGAAGAGGT AGTGAAGGCA CAAAAGTCTC TGCACATGTG TGTATTGGGG 
AACACTACTA TTCTTGCTGA GTTTGCCAGT GAAGAGGAGA TCAGCCGTTT CTTTGCACAA 
AGTCAGTCTC TGACCCCTTC TCCCGGCTGG CAGTCTCTCG GGTCCAGCCA GAGCCGGCTG 
GGCTCCCTCG ACTGTTCCCA CTCGTTCTCC AGCCGGACCG ATCTCAATCA CTGGAATGGT 
GCTGGGCTGT CGGGAACTAA CTGTGGAGAC CTTCACGGCA CTTCACTCTG GGGGACCCCA 
CATTATTCCA CAAGCCTGTG GGGTCCTCCC AGCAGCAGCG ACCCCCGGGG AATTAGCAGC 
CCATCCCCCA TTAATGCTTT TCTTTCTGTT GACCACCTGG GTGGGGGTGG AGAGTCCATG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_005621401.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005621458.1 Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X3, mRNA.

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CloneID	OCe07958
Clone ID Related Accession (Same CDS sequence)	XM_005621401.3 , XM_005621401.2
Accession Version	XM_005621401.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	trinucleotide repeat-containing gene 6A protein isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005621401.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACTAG TGGCAGAGCC AGGCCTAGAA GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG AGAATTGGAA GCTAAAGCTA CCAAAGACGT AGAAAGGACT 
CTTAGCAGGG ATTTAGTGCA AGAAGAAGAA CAGTTGATGG AAGAAAAGAA AAAGAAAAAA 
GACGACAAGA AAAAGAAGGA AGCTGCTCAA AAGAAGGCCA CTGAACAAAA AATCAAAGTG 
CCAGAACAGA TAAAGCCCAG TGTAAGCCAG CCTCAGCCTG CCAACTCTAA TAACGGCACT 
TCCACAGCAA CCAGCACTAA TAATAATGCC AAGCGAGCCA CAGCCAACAA TCCGCAGCAG 
CAGCAGCAGC CACAGCCGCC ACAGCAGCCA CAGCAGCCGC CGCAGCAGCC ACCGCAGCCG 
CCACAGCAGC AGCCGCAGCC AGCACAGGCC TTGCCTCGGT ATCCTCGGGA AGTACCACCA 
CGATTTCGCC ACCAGGAACA CAAACAGCTT CTAAAGAGGG GTCAGCATTT TCCTGTCATA 
GCAGCAAACC TGGGATCTGC TGTTAAAGTG TTAAGCAGCC AGTCAGAAAG CAGTGCTTTA 
ACAAATCAAC AGCCACAAAA TAACGGAGAG GTGCAGAACA GCAAAAACCA GTCAGATATA 
AACCACAGTA CTTCAGGATC CCATTATGAA AATTCACAGC GGGGACCTGT GTCTTCTCCG 
AGTGACTCTA GCACAAATTG TAAGAATGCT GTTGTAAACG ACTCCCCTGA GACAGAAGCA 
TGGCCCTCAG TCCCTGGCAG TGATCCAGAG TTGGCTTCAG AATGTATGGA TGCTGATTCT 
GCCTCCAGTT CTGAATCAGA GAGAAACATC ACTATCATGG CTTCAGGGAG CACAGGTGGT 
GAAAAAGATG GCCTTCGGAA TAGCACTGGA CTTGGTTCCC AAAACAAGTT TGTGGTTGGT 
AGCAGCAGCA ATAATGTGGG CCATGGAAGT AGTCCTGGGC CATGGGGTTT TTCCCATGGA 
GCCATAATAA GCACATGTCA GGTCTCTGTG GATGCTCCTG AAAGCAAATC AGAAAGTAGC 
AACAATAGAA TGAATGCTTG GGGCACTGTA AGTTCTTCAT CAAATGGAGG GTTAAATCCA 
AGCACTTTGA ATTCAGCTAG CAACCATGGT GCCTGGCCAG TATTAGACAA CAACGGACTT 
GCCCTCAAAG GGCCTGTAGG GAGCGGTAGT TCTGGCATCA ATATTCAGTG TAGTACCATA 
GGCCAGATGC CTAACAATCA GAGTATTAAC TCTAAAGTGG GTGGTTCTTC CACCCATGGT 
ACCTGGGGAA GCCTTCAGGA AACTTGTGAA TCTGAAGTAA GTGGTACACA GAAGGTTTCA 
TTCAGTGGTC AACCTCAGAA TATTACCACT GAGATGACTG GACCAAATAA CACTACTAAC 
TTTATGACCT CTAGTTTACC AAACTCCGGT TCAGTACAGA ATAACGAACT GCCTAGTAAT 
ACAGGGGCCT GGCGTGTGAG CACAATGAAT CATCCTCAGA TACAGGCTCC ATCGGTTATG 
AATGGCACTT CCCTTTCTCA TCTTAGCAAT GGAGAGCCAA AAAGTGGAGG CTCTTACGGT 
ACTACGTGGG GTGCCTATGG TTCTAATTAC TCTGGAGACA AATGTTCGAG CCCTAACGGC 
CAAGCTAATG GTGACACTGT GAATGCAACT CTAATGCAGC CTGGCATAAA TGGGCCTATG 
GGCACTAACT TTCAAGTGAA CACAAATAAA GGAGGAGGTG TGTGGGAGTC TGGGGCAACG 
AATTCCCAGA ATGCATCGTG GGGAAGTGGA AATGGCGCAA ATTCTGGAGG AAGTCGACGA 
GGATGGGGAA CTCCTGCACA AAACACTGGC ACTAATATAC CCAGCGTGGA GTGGAGCAAA 
CTGCCTAGCA ATCAGCATTC CAATGACAGT GCAAATGGCA ATGGTAAGAA GTTTACAAAT 
GGATGGAAAT CTACTGAGGA AGAGGATCAG GCTTCTGCCG CATCTCAGAC AACTGAGCAG 
AACAGTGTGT GGGCCAAAAC AGGAGGTACA GTGGAGAGTG AAGGTAGTAC AGAAAGCACT 
GGACGCCTCG AGGAGAAAGC CACTGGGGAA AATCAGAGTA GAGATCGGAG AAAAATTGAT 
CAGCACACAT TACTCCAAAG CATCGTAAAC AGAACTGACT TGGATCCACG TGTCCTCTCC 
AACTCTGGTT GGGGACAGAC TCCTATTAAG CAGAATACTG CCTGGGATAC GGAAACATCA 
CCTAGAGGGG AAAGAAAGAC TGACAATGGG ACAGAGGCCT GGGGAAGCTC TGCAACACAG 
ACTTTTAACT CAGGGGCATG TACAGATAAG ACTAGCCCTA CTAGTAATGA TACCTCATCT 
GTATCGGGGT GGGGAGATCC CAAACCTGCT CTGAGGTGGG GAGATTCCAA AGGCTCAAGC 
TGCCAGGGGG GATGGGAAGA TGATGCTGCT GCTACAGGAA TGGTCAAGAG CAATCAGTGG 
GGGAACAGCA AAGAAGAGAA GTCCGCATGG AATGATTCGC AAAAGAGCAA ACAGGGATGG 
GGTGATGGAC AGAAATCAAA CCAAGGGTGG TCCGTTTCTG CCAGTGATAA CTGGGGAGAA 
ACGTCAAGGA GTAACCATTG GGGTGAGGCT AATAAGAAAT CTAGCTCAGG AGGTAGTGAC 
AGTGACAGGT CTGTTTCTGG TTGGAATGAA CTTGGTAAAA CTAGTTCTTT TACTTGGGGA 
AATAACATAA ATCCAAATAA TTCGTCAGGA TGGGATGAAT CTTCTAAACC TAATCCTTCC 
CAGGGATGGG GAGATCCTCC AAAGTCTAAT CAGTCTCTAG GTTGGGGAGA TTCGTCAAAG 
CCAGTCACTT CTCCAGACTG GAACAAGCAA CAAGACATTG TCGGGTCTTG GGGAATCCCA 
CCAGCAACAG GCAAACCTCC TGGTACAGGC TGGCTGGGAG GACCTATACC AGCCCCAACA 
AAAGAAGAAG AGCCCACAGG CTGGGAGGAA CCATCCCCAG AATCCATACG TCGCAAAATG 
GAGATCGATG ACGGAACTTC AGCTTGGGGA GATCCGAGCA AATACAACTA CAAAAATGTG 
AACATGTGGA ACAAAAATGT CCCAAATGGC AGCAGCCGTT CAGACCAGCA AGCACAGGTA 
CACCAGTTGC TACCATCTGC AAGTGCCATC TCAAACAAAG AGGCAGGCAG TGGCTCTGGC 
TGGGGTGAGC CCTGGGGGGA GCCTTCTACT CCAGCCACAA CTGTGGATAA TGGTACTTCA 
GCATGGGGTA AACCCATAGA CAGTGGTCCC AGCTGGGGGG AGCCCATTGC TGCAGCATCC 
AACACATCCA CGTGGGGCTC CAGCTCTGTT GGTCCTCAAG CATTAAGCAA ATCTGGGCCA 
AAATCTATGC AAGATGGCTG GTGTGGTGAT GATATGCCAT TGCCTGGAAA TCGCCCCACT 
GGCTGGGAAG AGGAAGAGGA TGTAGAGATT GGAATGTGGA ATAGTAATTC ATCTCAAGAG 
CTTAACTCAT CTTTAAATTG GCCTCCATAT ACAAAGAAAA TGTCATCGAA GGGTCTGAGT 
GGCAAAAAAA GGAGAAGGGA AAGGGGAACC ATGAAAGGTG GAAACAAACA AGAAGAAGCA 
TGGATAAATC CATTCGTTAA ACAGTTTTCA AATATCAGTT TTTCGAGAGA CTCACCCGAA 
GAAAATGTAC AGAGCAATAA GATGGATCTT TCCGGAGGAA TGTTACAGGA CAAACGAATG 
GAGATAGATA AACATAGCCT AAATATTGGT GATTACAATC GAACGGTCGG GAAAGGCCCT 
GGTTCTCGGC CTCAGATTTC CAAAGAGTCT TCCATGGAGC GCAGCCCTTA CTTTGATAAG 
GATGGCATTG TAGCAGATGA ATCCCAAAAC ATGCAGTTTA TGTCCAGTCA AAGCATGAAG 
CTTCCCCCTT CAAATAGTGC ACTACCTAAC CAGGCCCTTG GCTCCATAGC AGGGCTGGGT 
ATGCAAAACT TGAATTCTGT TAGACAGAAT GGCAATCCCA GTATGTTTGG TGTTGGAAAC 
ACAGCAGCAC AACCCCGGGG CATGCAGCAG CCTCCAGCAC AACCTCTTAG TTCATCTCAG 
CCTAATCTTC GTGCTCAAGT GCCTCCTCCA TTACTCTCCC CTCAGGTAGC CATGCTGAAC 
CAGCTATCCC AACTAAACCA GCTTTCTCAG ATCTCCCAGT TACAGAGATT GTTAGCGCAG 
CAGCAAAGGG CGCAGAGTCA GAGAAGCGTG CCTTCTGGGA ACCGGCAGCA GCAAGACCAG 
CAGGGTCGAC CTCTTAGCGT GCAGCAGCAG ATGATGCAGC AGTCCCGGCA ACTCGATCCC 
AGCCTGTTGG TGAAGCAGCA GACCCCGCCA TCTCAGCAGC AGCCACTCCA TCAGCCAGCC 
ATGAAATCCT TCCTCGAGAA TGTCATGCCC CACACTACAC CTGAGCTGCA GAAAGGGCCA 
TCACCAATAA ATGCTTTCAG CAACTTCCCG ATAGGCTTGA ACTCAAACTT GAATGTAAAT 
ATGGATATGA ACAGTATTAA AGAGCCACAG TCAAGACTAA GGAAGTGGAC TACAGTGGAC 
AGCATTTCTG TGAACACATC TTTGGATCAA AACTCCAGCA AACATGGTGC TATTTCAAGT 
GGTTTCAGGC TGGAAGAGTC TCCATTTGTT CCCTATGACT TTATGAACAG CAGTACTTCT 
CCAGCCAGCC CTCCGGGTTC AATAGGAGAT GGCTGGCCAC GTGCCAAATC GCCTAACGGC 
TCTAGCAGTG TTAACTGGCC ACCAGAATTT CGCCCTGGTG AACCATGGAA AGGTTATCCA 
AACATTGACC CTGAAACTGA CCCTTACGTC ACTCCTGGCA GTGTCATAAA CAATCTTTCA 
ATTAATACTG TGCGGGAAGT TGACCACCTC AGGGACAGGA ACAGTGGGTC ATCCTCATCC 
TTGAACACCA CGCTGCCTTC AACTAGTGCC TGGTCATCCA TTCGTGCCTC CAACTACAAC 
GTTCCCCTCA GCAGTACAGC ACAAAGCACT TCAGCCAGAA ATAGTGATTC CAAATTGACA 
TGGTCTCCTG GTTCAGTTAC AAACACCTCT CTGGCTCATG AGCTGTGGAA GGTCCCTTTA 
CCACCTAAAA ACATCACTGC TCCGTCCCGC CCACCTCCGG GACTCACTGG TCAGAAGCCA 
CCCTTGTCTA CGTGGGATAA CTCTCCCCTT CGTGTAGGTG GAGGATGGGG AAATTCTGAC 
GCCAGGTACA CCCCAGGTTC CAGTTGGGGT GAGAGCAGCT CAGGGAGAAT AACAAATTGG 
CTTGTTCTAA AGAACCTCAC ACCTCAGATT GACGGCTCAA CTCTGCGTAC TCTGTGCATG 
CAGCATGGCC CACTGATAAC ATTCCACCTG AACCTCCCAC ATGGAAATGC TTTGGTCCGT 
TACAGTTCAA AAGAAGAGGT AGTGAAGGCA CAAAAGTCTC TGCACATGTG TGTATTGGGG 
AACACTACTA TTCTTGCTGA GTTTGCCAGT GAAGAGGAGA TCAGCCGTTT CTTTGCACAA 
AGTCAGTCTC TGACCCCTTC TCCCGGCTGG CAGTCTCTCG GGTCCAGCCA GAGCCGGCTG 
GGCTCCCTCG ACTGTTCCCA CTCGTTCTCC AGCCGGACCG ATCTCAATCA CTGGAATGGT 
GCTGGGCTGT CGGGAACTAA CTGTGGAGAC CTTCACGGCA CTTCACTCTG GGGGACCCCA 
CATTATTCCA CAAGCCTGTG GGGTCCTCCC AGCAGCAGCG ACCCCCGGGG AATTAGCAGC 
CCATCCCCCA TTAATGCTTT TCTTTCTGTT GACCACCTGG GTGGGGGTGG AGAGTCCATG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005621458.1
CDS	283..6165
Translation	MQLVAEPGLEARCPGSQDIPLPEEWNRELEAKATKDVERTLSRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNPQQQQQPQPPQQPQQPPQQPPQPPQQQPQPAQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLSSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSPSDSSTNCKNAVVNDSPETEAWPSVPGSDPELASECMDADSASSSESERNITIMASGSTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSPGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLDNNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVGGSSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVMNGTSLSHLSNGEPKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGINGPMGTNFQVNTNKGGGVWESGATNSQNASWGSGNGANSGGSRRGWGTPAQNTGTNIPSVEWSKLPSNQHSNDSANGNGKKFTNGWKSTEEEDQASAASQTTEQNSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACTDKTSPTSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDAAATGMVKSNQWGNSKEEKSAWNDSQKSKQGWGDGQKSNQGWSVSASDNWGETSRSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVTSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPTKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSASAISNKEAGSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASNTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGTMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERSPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPSLLVKQQTPPSQQQPLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_005621401.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005621401.2

ORF Insert Sequence:

ATGCAACTAG TGGCAGAGCC AGGCCTAGAA GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG AGAATTGGAA GCTAAAGCTA CCAAAGACGT AGAAAGGACT 
CTTAGCAGGG ATTTAGTGCA AGAAGAAGAA CAGTTGATGG AAGAAAAGAA AAAGAAAAAA 
GACGACAAGA AAAAGAAGGA AGCTGCTCAA AAGAAGGCCA CTGAACAAAA AATCAAAGTG 
CCAGAACAGA TAAAGCCCAG TGTAAGCCAG CCTCAGCCTG CCAACTCTAA TAACGGCACT 
TCCACAGCAA CCAGCACTAA TAATAATGCC AAGCGAGCCA CAGCCAACAA TCCGCAGCAG 
CAGCAGCAGC CACAGCCGCC ACAGCAGCCA CAGCAGCCGC CGCAGCAGCC ACCGCAGCCG 
CCACAGCAGC AGCCGCAGCC AGCACAGGCC TTGCCTCGGT ATCCTCGGGA AGTACCACCA 
CGATTTCGCC ACCAGGAACA CAAACAGCTT CTAAAGAGGG GTCAGCATTT TCCTGTCATA 
GCAGCAAACC TGGGATCTGC TGTTAAAGTG TTAAGCAGCC AGTCAGAAAG CAGTGCTTTA 
ACAAATCAAC AGCCACAAAA TAACGGAGAG GTGCAGAACA GCAAAAACCA GTCAGATATA 
AACCACAGTA CTTCAGGATC CCATTATGAA AATTCACAGC GGGGACCTGT GTCTTCTCCG 
AGTGACTCTA GCACAAATTG TAAGAATGCT GTTGTAAACG ACTCCCCTGA GACAGAAGCA 
TGGCCCTCAG TCCCTGGCAG TGATCCAGAG TTGGCTTCAG AATGTATGGA TGCTGATTCT 
GCCTCCAGTT CTGAATCAGA GAGAAACATC ACTATCATGG CTTCAGGGAG CACAGGTGGT 
GAAAAAGATG GCCTTCGGAA TAGCACTGGA CTTGGTTCCC AAAACAAGTT TGTGGTTGGT 
AGCAGCAGCA ATAATGTGGG CCATGGAAGT AGTCCTGGGC CATGGGGTTT TTCCCATGGA 
GCCATAATAA GCACATGTCA GGTCTCTGTG GATGCTCCTG AAAGCAAATC AGAAAGTAGC 
AACAATAGAA TGAATGCTTG GGGCACTGTA AGTTCTTCAT CAAATGGAGG GTTAAATCCA 
AGCACTTTGA ATTCAGCTAG CAACCATGGT GCCTGGCCAG TATTAGACAA CAACGGACTT 
GCCCTCAAAG GGCCTGTAGG GAGCGGTAGT TCTGGCATCA ATATTCAGTG TAGTACCATA 
GGCCAGATGC CTAACAATCA GAGTATTAAC TCTAAAGTGG GTGGTTCTTC CACCCATGGT 
ACCTGGGGAA GCCTTCAGGA AACTTGTGAA TCTGAAGTAA GTGGTACACA GAAGGTTTCA 
TTCAGTGGTC AACCTCAGAA TATTACCACT GAGATGACTG GACCAAATAA CACTACTAAC 
TTTATGACCT CTAGTTTACC AAACTCCGGT TCAGTACAGA ATAACGAACT GCCTAGTAAT 
ACAGGGGCCT GGCGTGTGAG CACAATGAAT CATCCTCAGA TACAGGCTCC ATCGGTTATG 
AATGGCACTT CCCTTTCTCA TCTTAGCAAT GGAGAGCCAA AAAGTGGAGG CTCTTACGGT 
ACTACGTGGG GTGCCTATGG TTCTAATTAC TCTGGAGACA AATGTTCGAG CCCTAACGGC 
CAAGCTAATG GTGACACTGT GAATGCAACT CTAATGCAGC CTGGCATAAA TGGGCCTATG 
GGCACTAACT TTCAAGTGAA CACAAATAAA GGAGGAGGTG TGTGGGAGTC TGGGGCAACG 
AATTCCCAGA ATGCATCGTG GGGAAGTGGA AATGGCGCAA ATTCTGGAGG AAGTCGACGA 
GGATGGGGAA CTCCTGCACA AAACACTGGC ACTAATATAC CCAGCGTGGA GTGGAGCAAA 
CTGCCTAGCA ATCAGCATTC CAATGACAGT GCAAATGGCA ATGGTAAGAA GTTTACAAAT 
GGATGGAAAT CTACTGAGGA AGAGGATCAG GCTTCTGCCG CATCTCAGAC AACTGAGCAG 
AACAGTGTGT GGGCCAAAAC AGGAGGTACA GTGGAGAGTG AAGGTAGTAC AGAAAGCACT 
GGACGCCTCG AGGAGAAAGC CACTGGGGAA AATCAGAGTA GAGATCGGAG AAAAATTGAT 
CAGCACACAT TACTCCAAAG CATCGTAAAC AGAACTGACT TGGATCCACG TGTCCTCTCC 
AACTCTGGTT GGGGACAGAC TCCTATTAAG CAGAATACTG CCTGGGATAC GGAAACATCA 
CCTAGAGGGG AAAGAAAGAC TGACAATGGG ACAGAGGCCT GGGGAAGCTC TGCAACACAG 
ACTTTTAACT CAGGGGCATG TACAGATAAG ACTAGCCCTA CTAGTAATGA TACCTCATCT 
GTATCGGGGT GGGGAGATCC CAAACCTGCT CTGAGGTGGG GAGATTCCAA AGGCTCAAGC 
TGCCAGGGGG GATGGGAAGA TGATGCTGCT GCTACAGGAA TGGTCAAGAG CAATCAGTGG 
GGGAACAGCA AAGAAGAGAA GTCCGCATGG AATGATTCGC AAAAGAGCAA ACAGGGATGG 
GGTGATGGAC AGAAATCAAA CCAAGGGTGG TCCGTTTCTG CCAGTGATAA CTGGGGAGAA 
ACGTCAAGGA GTAACCATTG GGGTGAGGCT AATAAGAAAT CTAGCTCAGG AGGTAGTGAC 
AGTGACAGGT CTGTTTCTGG TTGGAATGAA CTTGGTAAAA CTAGTTCTTT TACTTGGGGA 
AATAACATAA ATCCAAATAA TTCGTCAGGA TGGGATGAAT CTTCTAAACC TAATCCTTCC 
CAGGGATGGG GAGATCCTCC AAAGTCTAAT CAGTCTCTAG GTTGGGGAGA TTCGTCAAAG 
CCAGTCACTT CTCCAGACTG GAACAAGCAA CAAGACATTG TCGGGTCTTG GGGAATCCCA 
CCAGCAACAG GCAAACCTCC TGGTACAGGC TGGCTGGGAG GACCTATACC AGCCCCAACA 
AAAGAAGAAG AGCCCACAGG CTGGGAGGAA CCATCCCCAG AATCCATACG TCGCAAAATG 
GAGATCGATG ACGGAACTTC AGCTTGGGGA GATCCGAGCA AATACAACTA CAAAAATGTG 
AACATGTGGA ACAAAAATGT CCCAAATGGC AGCAGCCGTT CAGACCAGCA AGCACAGGTA 
CACCAGTTGC TACCATCTGC AAGTGCCATC TCAAACAAAG AGGCAGGCAG TGGCTCTGGC 
TGGGGTGAGC CCTGGGGGGA GCCTTCTACT CCAGCCACAA CTGTGGATAA TGGTACTTCA 
GCATGGGGTA AACCCATAGA CAGTGGTCCC AGCTGGGGGG AGCCCATTGC TGCAGCATCC 
AACACATCCA CGTGGGGCTC CAGCTCTGTT GGTCCTCAAG CATTAAGCAA ATCTGGGCCA 
AAATCTATGC AAGATGGCTG GTGTGGTGAT GATATGCCAT TGCCTGGAAA TCGCCCCACT 
GGCTGGGAAG AGGAAGAGGA TGTAGAGATT GGAATGTGGA ATAGTAATTC ATCTCAAGAG 
CTTAACTCAT CTTTAAATTG GCCTCCATAT ACAAAGAAAA TGTCATCGAA GGGTCTGAGT 
GGCAAAAAAA GGAGAAGGGA AAGGGGAACC ATGAAAGGTG GAAACAAACA AGAAGAAGCA 
TGGATAAATC CATTCGTTAA ACAGTTTTCA AATATCAGTT TTTCGAGAGA CTCACCCGAA 
GAAAATGTAC AGAGCAATAA GATGGATCTT TCCGGAGGAA TGTTACAGGA CAAACGAATG 
GAGATAGATA AACATAGCCT AAATATTGGT GATTACAATC GAACGGTCGG GAAAGGCCCT 
GGTTCTCGGC CTCAGATTTC CAAAGAGTCT TCCATGGAGC GCAGCCCTTA CTTTGATAAG 
GATGGCATTG TAGCAGATGA ATCCCAAAAC ATGCAGTTTA TGTCCAGTCA AAGCATGAAG 
CTTCCCCCTT CAAATAGTGC ACTACCTAAC CAGGCCCTTG GCTCCATAGC AGGGCTGGGT 
ATGCAAAACT TGAATTCTGT TAGACAGAAT GGCAATCCCA GTATGTTTGG TGTTGGAAAC 
ACAGCAGCAC AACCCCGGGG CATGCAGCAG CCTCCAGCAC AACCTCTTAG TTCATCTCAG 
CCTAATCTTC GTGCTCAAGT GCCTCCTCCA TTACTCTCCC CTCAGGTAGC CATGCTGAAC 
CAGCTATCCC AACTAAACCA GCTTTCTCAG ATCTCCCAGT TACAGAGATT GTTAGCGCAG 
CAGCAAAGGG CGCAGAGTCA GAGAAGCGTG CCTTCTGGGA ACCGGCAGCA GCAAGACCAG 
CAGGGTCGAC CTCTTAGCGT GCAGCAGCAG ATGATGCAGC AGTCCCGGCA ACTCGATCCC 
AGCCTGTTGG TGAAGCAGCA GACCCCGCCA TCTCAGCAGC AGCCACTCCA TCAGCCAGCC 
ATGAAATCCT TCCTCGAGAA TGTCATGCCC CACACTACAC CTGAGCTGCA GAAAGGGCCA 
TCACCAATAA ATGCTTTCAG CAACTTCCCG ATAGGCTTGA ACTCAAACTT GAATGTAAAT 
ATGGATATGA ACAGTATTAA AGAGCCACAG TCAAGACTAA GGAAGTGGAC TACAGTGGAC 
AGCATTTCTG TGAACACATC TTTGGATCAA AACTCCAGCA AACATGGTGC TATTTCAAGT 
GGTTTCAGGC TGGAAGAGTC TCCATTTGTT CCCTATGACT TTATGAACAG CAGTACTTCT 
CCAGCCAGCC CTCCGGGTTC AATAGGAGAT GGCTGGCCAC GTGCCAAATC GCCTAACGGC 
TCTAGCAGTG TTAACTGGCC ACCAGAATTT CGCCCTGGTG AACCATGGAA AGGTTATCCA 
AACATTGACC CTGAAACTGA CCCTTACGTC ACTCCTGGCA GTGTCATAAA CAATCTTTCA 
ATTAATACTG TGCGGGAAGT TGACCACCTC AGGGACAGGA ACAGTGGGTC ATCCTCATCC 
TTGAACACCA CGCTGCCTTC AACTAGTGCC TGGTCATCCA TTCGTGCCTC CAACTACAAC 
GTTCCCCTCA GCAGTACAGC ACAAAGCACT TCAGCCAGAA ATAGTGATTC CAAATTGACA 
TGGTCTCCTG GTTCAGTTAC AAACACCTCT CTGGCTCATG AGCTGTGGAA GGTCCCTTTA 
CCACCTAAAA ACATCACTGC TCCGTCCCGC CCACCTCCGG GACTCACTGG TCAGAAGCCA 
CCCTTGTCTA CGTGGGATAA CTCTCCCCTT CGTGTAGGTG GAGGATGGGG AAATTCTGAC 
GCCAGGTACA CCCCAGGTTC CAGTTGGGGT GAGAGCAGCT CAGGGAGAAT AACAAATTGG 
CTTGTTCTAA AGAACCTCAC ACCTCAGATT GACGGCTCAA CTCTGCGTAC TCTGTGCATG 
CAGCATGGCC CACTGATAAC ATTCCACCTG AACCTCCCAC ATGGAAATGC TTTGGTCCGT 
TACAGTTCAA AAGAAGAGGT AGTGAAGGCA CAAAAGTCTC TGCACATGTG TGTATTGGGG 
AACACTACTA TTCTTGCTGA GTTTGCCAGT GAAGAGGAGA TCAGCCGTTT CTTTGCACAA 
AGTCAGTCTC TGACCCCTTC TCCCGGCTGG CAGTCTCTCG GGTCCAGCCA GAGCCGGCTG 
GGCTCCCTCG ACTGTTCCCA CTCGTTCTCC AGCCGGACCG ATCTCAATCA CTGGAATGGT 
GCTGGGCTGT CGGGAACTAA CTGTGGAGAC CTTCACGGCA CTTCACTCTG GGGGACCCCA 
CATTATTCCA CAAGCCTGTG GGGTCCTCCC AGCAGCAGCG ACCCCCGGGG AATTAGCAGC 
CCATCCCCCA TTAATGCTTT TCTTTCTGTT GACCACCTGG GTGGGGGTGG AGAGTCCATG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_005621402.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005621459.1 Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X4, mRNA.

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CloneID	OCe07959
Clone ID Related Accession (Same CDS sequence)	XM_005621402.2 , XM_005621402.3
Accession Version	XM_005621402.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5805bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	trinucleotide repeat-containing gene 6A protein isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005621402.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACTAG TGGCAGAGCC AGGCCTAGAA GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG AGAATTGGAA GCTAAAGCTA CCAAAGACGT AGAAAGGACT 
CTTAGCAGGG ATTTAGTGCA AGAAGAAGAA CAGTTGATGG AAGAAAAGAA AAAGAAAAAA 
GACGACAAGA AAAAGAAGGA AGCTGCTCAA AAGAAGGCCA CTGAACAAAA AATCAAAGTG 
CCAGAACAGA TAAAGCCCAG TGTAAGCCAG CCTCAGCCTG CCAACTCTAA TAACGGCACT 
TCCACAGCAA CCAGCACTAA TAATAATGCC AAGCGAGCCA CAGCCAACAA TCCGCAGCAG 
CAGCAGCAGC CACAGCCGCC ACAGCAGCCA CAGCAGCCGC CGCAGCAGCC ACCGCAGCCG 
CCACAGCAGC AGCCGCAGCC AGCACAGGCC TTGCCTCGGT ATCCTCGGGA AGTACCACCA 
CGATTTCGCC ACCAGGAACA CAAACAGCTT CTAAAGAGGG GTCAGCATTT TCCTGTCATA 
GCAGCAAACC TGGGATCTGC TGTTAAAGTG TTAAGCAGCC AGTCAGAAAG CAGTGCTTTA 
ACAAATCAAC AGCCACAAAA TAACGGAGAG GTGCAGAACA GCAAAAACCA GTCAGATATA 
AACCACAGTA CTTCAGGATC CCATTATGAA AATTCACAGC GGGGACCTGT GTCTTCTCCG 
AGTGACTCTA GCACAAATTG TAAGAATGCT GTTGTAAACG ACTCCCCTGA GACAGAAGCA 
TGGCCCTCAG TCCCTGGCAG TGATCCAGAG TTGGCTTCAG AATGTATGGA TGCTGATTCT 
GCCTCCAGTT CTGAATCAGA GAGAAACATC ACTATCATGG CTTCAGGGAG CACAGGTGGT 
GAAAAAGATG GCCTTCGGAA TAGCACTGGA CTTGGTTCCC AAAACAAGTT TGTGGTTGGT 
AGCAGCAGCA ATAATGTGGG CCATGGAAGT AGTCCTGGGC CATGGGGTTT TTCCCATGGA 
GCCATAATAA GCACATGTCA GGTCTCTGTG GATGCTCCTG AAAGCAAATC AGAAAGTAGC 
AACAATAGAA TGAATGCTTG GGGCACTGTA AGTTCTTCAT CAAATGGAGG GTTAAATCCA 
AGCACTTTGA ATTCAGCTAG CAACCATGGT GCCTGGCCAG TATTAGACAA CAACGGACTT 
GCCCTCAAAG GGCCTGTAGG GAGCGGTAGT TCTGGCATCA ATATTCAGTG TAGTACCATA 
GGCCAGATGC CTAACAATCA GAGTATTAAC TCTAAAGTGG GTGGTTCTTC CACCCATGGT 
ACCTGGGGAA GCCTTCAGGA AACTTGTGAA TCTGAAGTAA GTGGTACACA GAAGGTTTCA 
TTCAGTGGTC AACCTCAGAA TATTACCACT GAGATGACTG GACCAAATAA CACTACTAAC 
TTTATGACCT CTAGTTTACC AAACTCCGGT TCAGTACAGA ATAACGAACT GCCTAGTAAT 
ACAGGGGCCT GGCGTGTGAG CACAATGAAT CATCCTCAGA TACAGGCTCC ATCGGTTATG 
AATGGCACTT CCCTTTCTCA TCTTAGCAAT GGAGAGCCAA AAAGTGGAGG CTCTTACGGT 
ACTACGTGGG GTGCCTATGG TTCTAATTAC TCTGGAGACA AATGTTCGAG CCCTAACGGC 
CAAGCTAATG GTGACACTGT GAATGCAACT CTAATGCAGC CTGGCATAAA TGGGCCTATG 
GGCACTAACT TTCAAGTGAA CACAAATAAA GGAGGAGGTG TGTGGGAGTC TGGGGCAACG 
AATTCCCAGA ATGCATCGTG GGGAAGTGGA AATGGCGCAA ATTCTGGAGG AAGTCGACGA 
GGATGGGGAA CTCCTGCACA AAACACTGGC ACTAATATAC CCAGCGTGGA GTGGAGCAAA 
CTGCCTAGCA ATCAGCATTC CAATGACAGT GCAAATGGCA ATGGTAAGAA GTTTACAAAT 
GGATGGAAAT CTACTGAGGA AGAGGATCAG GCTTCTGCCG CATCTCAGAC AACTGAGCAG 
AACAGTGTGT GGGCCAAAAC AGGAGGTACA GTGGAGAGTG AAGGTAGTAC AGAAAGCACT 
GGACGCCTCG AGGAGAAAGC CACTGGGGAA AATCAGAGTA GAGATCGGAG AAAAATTGAT 
CAGCACACAT TACTCCAAAG CATCGTAAAC AGAACTGACT TGGATCCACG TGTCCTCTCC 
AACTCTGGTT GGGGACAGAC TCCTATTAAG CAGAATACTG CCTGGGATAC GGAAACATCA 
CCTAGAGGGG AAAGAAAGAC TGACAATGGG ACAGAGGCCT GGGGAAGCTC TGCAACACAG 
ACTTTTAACT CAGGGGCATG TACAGATAAG ACTAGCCCTA CTAGTAATGA TACCTCATCT 
GTATCGGGGT GGGGAGATCC CAAACCTGCT CTGAGGTGGG GAGATTCCAA AGGCTCAAGC 
TGCCAGGGGG GATGGGAAGA TGATGCTGCT GCTACAGGAA TGGTCAAGAG CAATCAGTGG 
GGGAACAGCA AAGAAGAGAA GTCCGCATGG AATGATTCGC AAAAGAGCAA ACAGGGATGG 
GGTGATGGAC AGAAATCAAA CCAAGGGTGG TCCGTTTCTG CCAGTGATAA CTGGGGAGAA 
ACGTCAAGGA GTAACCATTG GGGTGAGGCT AATAAGAAAT CTAGCTCAGG AGGTAGTGAC 
AGTGACAGGT CTGTTTCTGG TTGGAATGAA CTTGGTAAAA CTAGTTCTTT TACTTGGGGA 
AATAACATAA ATCCAAATAA TTCGTCAGGA TGGGATGAAT CTTCTAAACC TAATCCTTCC 
CAGGGATGGG GAGATCCTCC AAAGTCTAAT CAGTCTCTAG GTTGGGGAGA TTCGTCAAAG 
CCAGTCACTT CTCCAGACTG GAACAAGCAA CAAGACATTG TCGGGTCTTG GGGAATCCCA 
CCAGCAACAG GCAAACCTCC TGGTACAGGC TGGCTGGGAG GACCTATACC AGCCCCAACA 
AAAGAAGAAG AGCCCACAGG CTGGGAGGAA CCATCCCCAG AATCCATACG TCGCAAAATG 
GAGATCGATG ACGGAACTTC AGCTTGGGGA GATCCGAGCA AATACAACTA CAAAAATGTG 
AACATGTGGA ACAAAAATGT CCCAAATGGC AGCAGCCGTT CAGACCAGCA AGCACAGGTA 
CACCAGTTGC TACCATCTGC AAGTGCCATC TCAAACAAAG AGGCAGGCAG TGGCTCTGGC 
TGGGGTGAGC CCTGGGGGGA GCCTTCTACT CCAGCCACAA CTGTGGATAA TGGTACTTCA 
GCATGGGGTA AACCCATAGA CAGTGGTCCC AGCTGGGGGG AGCCCATTGC TGCAGCATCC 
AACACATCCA CGTGGGGCTC CAGCTCTGTT GGTCCTCAAG CATTAAGCAA ATCTGGGCCA 
AAATCTATGC AAGATGGCTG GTGTGGTGAT GATATGCCAT TGCCTGGAAA TCGCCCCACT 
GGCTGGGAAG AGGAAGAGGA TGTAGAGATT GGAATGTGGA ATAGTAATTC ATCTCAAGAG 
CTTAACTCAT CTTTAAATTG GCCTCCATAT ACAAAGAAAA TGTCATCGAA GGGTCTGAGT 
GGCAAAAAAA GGAGAAGGGA AAGGGGAACC ATGAAAGGTG GAAACAAACA AGAAGAAGCA 
TGGATAAATC CATTCGTTAA ACAGTTTTCA AATATCAGTT TTTCGAGAGA CTCACCCGAA 
GAAAATGTAC AGAGCAATAA GATGGATCTT TCCGGAGGAA TGTTACAGGA CAAACGAATG 
GAGATAGATA AACATAGCCT AAATATTGGT GATTACAATC GAACGGTCGG GAAAGGCCCT 
GGTTCTCGGC CTCAGATTTC CAAAGAGTCT TCCATGGAGC GCAGCCCTTA CTTTGATAAG 
AATGGCAATC CCAGTATGTT TGGTGTTGGA AACACAGCAG CACAACCCCG GGGCATGCAG 
CAGCCTCCAG CACAACCTCT TAGTTCATCT CAGCCTAATC TTCGTGCTCA AGTGCCTCCT 
CCATTACTCT CCCCTCAGGT TCCAGTTTCA TTGCTGAAGT ATGCACCAAA CAACGGTGGC 
CTGAATCCGC TCTTTGGCCC TCAACAGGTA GCCATGCTGA ACCAGCTATC CCAACTAAAC 
CAGCTTTCTC AGATCTCCCA GTTACAGAGA TTGTTAGCGC AGCAGCAAAG GGCGCAGAGT 
CAGAGAAGCG TGCCTTCTGG GAACCGGCAG CAGCAAGACC AGCAGGGTCG ACCTCTTAGC 
GTGCAGCAGC AGATGATGCA GCAGTCCCGG CAACTCGATC CCAGCCTGTT GGTGAAGCAG 
CAGACCCCGC CATCTCAGCA GCAGCCACTC CATCAGCCAG CCATGAAATC CTTCCTCGAG 
AATGTCATGC CCCACACTAC ACCTGAGCTG CAGAAAGGGC CATCACCAAT AAATGCTTTC 
AGCAACTTCC CGATAGGCTT GAACTCAAAC TTGAATGTAA ATATGGATAT GAACAGTATT 
AAAGAGCCAC AGTCAAGACT AAGGAAGTGG ACTACAGTGG ACAGCATTTC TGTGAACACA 
TCTTTGGATC AAAACTCCAG CAAACATGGT GCTATTTCAA GTGGTTTCAG GCTGGAAGAG 
TCTCCATTTG TTCCCTATGA CTTTATGAAC AGCAGTACTT CTCCAGCCAG CCCTCCGGGT 
TCAATAGGAG ATGGCTGGCC ACGTGCCAAA TCGCCTAACG GCTCTAGCAG TGTTAACTGG 
CCACCAGAAT TTCGCCCTGG TGAACCATGG AAAGGTTATC CAAACATTGA CCCTGAAACT 
GACCCTTACG TCACTCCTGG CAGTGTCATA AACAATCTTT CAATTAATAC TGTGCGGGAA 
GTTGACCACC TCAGGGACAG GAACAGTGGG TCATCCTCAT CCTTGAACAC CACGCTGCCT 
TCAACTAGTG CCTGGTCATC CATTCGTGCC TCCAACTACA ACGTTCCCCT CAGCAGTACA 
GCACAAAGCA CTTCAGCCAG AAATAGTGAT TCCAAATTGA CATGGTCTCC TGGTTCAGTT 
ACAAACACCT CTCTGGCTCA TGAGCTGTGG AAGGTCCCTT TACCACCTAA AAACATCACT 
GCTCCGTCCC GCCCACCTCC GGGACTCACT GGTCAGAAGC CACCCTTGTC TACGTGGGAT 
AACTCTCCCC TTCGTGTAGG TGGAGGATGG GGAAATTCTG ACGCCAGGTA CACCCCAGGT 
TCCAGTTGGG GTGAGAGCAG CTCAGGGAGA ATAACAAATT GGCTTGTTCT AAAGAACCTC 
ACACCTCAGA TTGACGGCTC AACTCTGCGT ACTCTGTGCA TGCAGCATGG CCCACTGATA 
ACATTCCACC TGAACCTCCC ACATGGAAAT GCTTTGGTCC GTTACAGTTC AAAAGAAGAG 
GTAGTGAAGG CACAAAAGTC TCTGCACATG TGTGTATTGG GGAACACTAC TATTCTTGCT 
GAGTTTGCCA GTGAAGAGGA GATCAGCCGT TTCTTTGCAC AAAGTCAGTC TCTGACCCCT 
TCTCCCGGCT GGCAGTCTCT CGGGTCCAGC CAGAGCCGGC TGGGCTCCCT CGACTGTTCC 
CACTCGTTCT CCAGCCGGAC CGATCTCAAT CACTGGAATG GTGCTGGGCT GTCGGGAACT 
AACTGTGGAG ACCTTCACGG CACTTCACTC TGGGGGACCC CACATTATTC CACAAGCCTG 
TGGGGTCCTC CCAGCAGCAG CGACCCCCGG GGAATTAGCA GCCCATCCCC CATTAATGCT 
TTTCTTTCTG TTGACCACCT GGGTGGGGGT GGAGAGTCCA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005621459.1
CDS	282..6086
Translation	MQLVAEPGLEARCPGSQDIPLPEEWNRELEAKATKDVERTLSRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNPQQQQQPQPPQQPQQPPQQPPQPPQQQPQPAQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLSSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSPSDSSTNCKNAVVNDSPETEAWPSVPGSDPELASECMDADSASSSESERNITIMASGSTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSPGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLDNNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVGGSSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVMNGTSLSHLSNGEPKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGINGPMGTNFQVNTNKGGGVWESGATNSQNASWGSGNGANSGGSRRGWGTPAQNTGTNIPSVEWSKLPSNQHSNDSANGNGKKFTNGWKSTEEEDQASAASQTTEQNSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACTDKTSPTSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDAAATGMVKSNQWGNSKEEKSAWNDSQKSKQGWGDGQKSNQGWSVSASDNWGETSRSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVTSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPTKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSASAISNKEAGSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASNTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGTMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERSPYFDKNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPSLLVKQQTPPSQQQPLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_005621402.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005621402.2

ORF Insert Sequence:

ATGCAACTAG TGGCAGAGCC AGGCCTAGAA GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG AGAATTGGAA GCTAAAGCTA CCAAAGACGT AGAAAGGACT 
CTTAGCAGGG ATTTAGTGCA AGAAGAAGAA CAGTTGATGG AAGAAAAGAA AAAGAAAAAA 
GACGACAAGA AAAAGAAGGA AGCTGCTCAA AAGAAGGCCA CTGAACAAAA AATCAAAGTG 
CCAGAACAGA TAAAGCCCAG TGTAAGCCAG CCTCAGCCTG CCAACTCTAA TAACGGCACT 
TCCACAGCAA CCAGCACTAA TAATAATGCC AAGCGAGCCA CAGCCAACAA TCCGCAGCAG 
CAGCAGCAGC CACAGCCGCC ACAGCAGCCA CAGCAGCCGC CGCAGCAGCC ACCGCAGCCG 
CCACAGCAGC AGCCGCAGCC AGCACAGGCC TTGCCTCGGT ATCCTCGGGA AGTACCACCA 
CGATTTCGCC ACCAGGAACA CAAACAGCTT CTAAAGAGGG GTCAGCATTT TCCTGTCATA 
GCAGCAAACC TGGGATCTGC TGTTAAAGTG TTAAGCAGCC AGTCAGAAAG CAGTGCTTTA 
ACAAATCAAC AGCCACAAAA TAACGGAGAG GTGCAGAACA GCAAAAACCA GTCAGATATA 
AACCACAGTA CTTCAGGATC CCATTATGAA AATTCACAGC GGGGACCTGT GTCTTCTCCG 
AGTGACTCTA GCACAAATTG TAAGAATGCT GTTGTAAACG ACTCCCCTGA GACAGAAGCA 
TGGCCCTCAG TCCCTGGCAG TGATCCAGAG TTGGCTTCAG AATGTATGGA TGCTGATTCT 
GCCTCCAGTT CTGAATCAGA GAGAAACATC ACTATCATGG CTTCAGGGAG CACAGGTGGT 
GAAAAAGATG GCCTTCGGAA TAGCACTGGA CTTGGTTCCC AAAACAAGTT TGTGGTTGGT 
AGCAGCAGCA ATAATGTGGG CCATGGAAGT AGTCCTGGGC CATGGGGTTT TTCCCATGGA 
GCCATAATAA GCACATGTCA GGTCTCTGTG GATGCTCCTG AAAGCAAATC AGAAAGTAGC 
AACAATAGAA TGAATGCTTG GGGCACTGTA AGTTCTTCAT CAAATGGAGG GTTAAATCCA 
AGCACTTTGA ATTCAGCTAG CAACCATGGT GCCTGGCCAG TATTAGACAA CAACGGACTT 
GCCCTCAAAG GGCCTGTAGG GAGCGGTAGT TCTGGCATCA ATATTCAGTG TAGTACCATA 
GGCCAGATGC CTAACAATCA GAGTATTAAC TCTAAAGTGG GTGGTTCTTC CACCCATGGT 
ACCTGGGGAA GCCTTCAGGA AACTTGTGAA TCTGAAGTAA GTGGTACACA GAAGGTTTCA 
TTCAGTGGTC AACCTCAGAA TATTACCACT GAGATGACTG GACCAAATAA CACTACTAAC 
TTTATGACCT CTAGTTTACC AAACTCCGGT TCAGTACAGA ATAACGAACT GCCTAGTAAT 
ACAGGGGCCT GGCGTGTGAG CACAATGAAT CATCCTCAGA TACAGGCTCC ATCGGTTATG 
AATGGCACTT CCCTTTCTCA TCTTAGCAAT GGAGAGCCAA AAAGTGGAGG CTCTTACGGT 
ACTACGTGGG GTGCCTATGG TTCTAATTAC TCTGGAGACA AATGTTCGAG CCCTAACGGC 
CAAGCTAATG GTGACACTGT GAATGCAACT CTAATGCAGC CTGGCATAAA TGGGCCTATG 
GGCACTAACT TTCAAGTGAA CACAAATAAA GGAGGAGGTG TGTGGGAGTC TGGGGCAACG 
AATTCCCAGA ATGCATCGTG GGGAAGTGGA AATGGCGCAA ATTCTGGAGG AAGTCGACGA 
GGATGGGGAA CTCCTGCACA AAACACTGGC ACTAATATAC CCAGCGTGGA GTGGAGCAAA 
CTGCCTAGCA ATCAGCATTC CAATGACAGT GCAAATGGCA ATGGTAAGAA GTTTACAAAT 
GGATGGAAAT CTACTGAGGA AGAGGATCAG GCTTCTGCCG CATCTCAGAC AACTGAGCAG 
AACAGTGTGT GGGCCAAAAC AGGAGGTACA GTGGAGAGTG AAGGTAGTAC AGAAAGCACT 
GGACGCCTCG AGGAGAAAGC CACTGGGGAA AATCAGAGTA GAGATCGGAG AAAAATTGAT 
CAGCACACAT TACTCCAAAG CATCGTAAAC AGAACTGACT TGGATCCACG TGTCCTCTCC 
AACTCTGGTT GGGGACAGAC TCCTATTAAG CAGAATACTG CCTGGGATAC GGAAACATCA 
CCTAGAGGGG AAAGAAAGAC TGACAATGGG ACAGAGGCCT GGGGAAGCTC TGCAACACAG 
ACTTTTAACT CAGGGGCATG TACAGATAAG ACTAGCCCTA CTAGTAATGA TACCTCATCT 
GTATCGGGGT GGGGAGATCC CAAACCTGCT CTGAGGTGGG GAGATTCCAA AGGCTCAAGC 
TGCCAGGGGG GATGGGAAGA TGATGCTGCT GCTACAGGAA TGGTCAAGAG CAATCAGTGG 
GGGAACAGCA AAGAAGAGAA GTCCGCATGG AATGATTCGC AAAAGAGCAA ACAGGGATGG 
GGTGATGGAC AGAAATCAAA CCAAGGGTGG TCCGTTTCTG CCAGTGATAA CTGGGGAGAA 
ACGTCAAGGA GTAACCATTG GGGTGAGGCT AATAAGAAAT CTAGCTCAGG AGGTAGTGAC 
AGTGACAGGT CTGTTTCTGG TTGGAATGAA CTTGGTAAAA CTAGTTCTTT TACTTGGGGA 
AATAACATAA ATCCAAATAA TTCGTCAGGA TGGGATGAAT CTTCTAAACC TAATCCTTCC 
CAGGGATGGG GAGATCCTCC AAAGTCTAAT CAGTCTCTAG GTTGGGGAGA TTCGTCAAAG 
CCAGTCACTT CTCCAGACTG GAACAAGCAA CAAGACATTG TCGGGTCTTG GGGAATCCCA 
CCAGCAACAG GCAAACCTCC TGGTACAGGC TGGCTGGGAG GACCTATACC AGCCCCAACA 
AAAGAAGAAG AGCCCACAGG CTGGGAGGAA CCATCCCCAG AATCCATACG TCGCAAAATG 
GAGATCGATG ACGGAACTTC AGCTTGGGGA GATCCGAGCA AATACAACTA CAAAAATGTG 
AACATGTGGA ACAAAAATGT CCCAAATGGC AGCAGCCGTT CAGACCAGCA AGCACAGGTA 
CACCAGTTGC TACCATCTGC AAGTGCCATC TCAAACAAAG AGGCAGGCAG TGGCTCTGGC 
TGGGGTGAGC CCTGGGGGGA GCCTTCTACT CCAGCCACAA CTGTGGATAA TGGTACTTCA 
GCATGGGGTA AACCCATAGA CAGTGGTCCC AGCTGGGGGG AGCCCATTGC TGCAGCATCC 
AACACATCCA CGTGGGGCTC CAGCTCTGTT GGTCCTCAAG CATTAAGCAA ATCTGGGCCA 
AAATCTATGC AAGATGGCTG GTGTGGTGAT GATATGCCAT TGCCTGGAAA TCGCCCCACT 
GGCTGGGAAG AGGAAGAGGA TGTAGAGATT GGAATGTGGA ATAGTAATTC ATCTCAAGAG 
CTTAACTCAT CTTTAAATTG GCCTCCATAT ACAAAGAAAA TGTCATCGAA GGGTCTGAGT 
GGCAAAAAAA GGAGAAGGGA AAGGGGAACC ATGAAAGGTG GAAACAAACA AGAAGAAGCA 
TGGATAAATC CATTCGTTAA ACAGTTTTCA AATATCAGTT TTTCGAGAGA CTCACCCGAA 
GAAAATGTAC AGAGCAATAA GATGGATCTT TCCGGAGGAA TGTTACAGGA CAAACGAATG 
GAGATAGATA AACATAGCCT AAATATTGGT GATTACAATC GAACGGTCGG GAAAGGCCCT 
GGTTCTCGGC CTCAGATTTC CAAAGAGTCT TCCATGGAGC GCAGCCCTTA CTTTGATAAG 
AATGGCAATC CCAGTATGTT TGGTGTTGGA AACACAGCAG CACAACCCCG GGGCATGCAG 
CAGCCTCCAG CACAACCTCT TAGTTCATCT CAGCCTAATC TTCGTGCTCA AGTGCCTCCT 
CCATTACTCT CCCCTCAGGT TCCAGTTTCA TTGCTGAAGT ATGCACCAAA CAACGGTGGC 
CTGAATCCGC TCTTTGGCCC TCAACAGGTA GCCATGCTGA ACCAGCTATC CCAACTAAAC 
CAGCTTTCTC AGATCTCCCA GTTACAGAGA TTGTTAGCGC AGCAGCAAAG GGCGCAGAGT 
CAGAGAAGCG TGCCTTCTGG GAACCGGCAG CAGCAAGACC AGCAGGGTCG ACCTCTTAGC 
GTGCAGCAGC AGATGATGCA GCAGTCCCGG CAACTCGATC CCAGCCTGTT GGTGAAGCAG 
CAGACCCCGC CATCTCAGCA GCAGCCACTC CATCAGCCAG CCATGAAATC CTTCCTCGAG 
AATGTCATGC CCCACACTAC ACCTGAGCTG CAGAAAGGGC CATCACCAAT AAATGCTTTC 
AGCAACTTCC CGATAGGCTT GAACTCAAAC TTGAATGTAA ATATGGATAT GAACAGTATT 
AAAGAGCCAC AGTCAAGACT AAGGAAGTGG ACTACAGTGG ACAGCATTTC TGTGAACACA 
TCTTTGGATC AAAACTCCAG CAAACATGGT GCTATTTCAA GTGGTTTCAG GCTGGAAGAG 
TCTCCATTTG TTCCCTATGA CTTTATGAAC AGCAGTACTT CTCCAGCCAG CCCTCCGGGT 
TCAATAGGAG ATGGCTGGCC ACGTGCCAAA TCGCCTAACG GCTCTAGCAG TGTTAACTGG 
CCACCAGAAT TTCGCCCTGG TGAACCATGG AAAGGTTATC CAAACATTGA CCCTGAAACT 
GACCCTTACG TCACTCCTGG CAGTGTCATA AACAATCTTT CAATTAATAC TGTGCGGGAA 
GTTGACCACC TCAGGGACAG GAACAGTGGG TCATCCTCAT CCTTGAACAC CACGCTGCCT 
TCAACTAGTG CCTGGTCATC CATTCGTGCC TCCAACTACA ACGTTCCCCT CAGCAGTACA 
GCACAAAGCA CTTCAGCCAG AAATAGTGAT TCCAAATTGA CATGGTCTCC TGGTTCAGTT 
ACAAACACCT CTCTGGCTCA TGAGCTGTGG AAGGTCCCTT TACCACCTAA AAACATCACT 
GCTCCGTCCC GCCCACCTCC GGGACTCACT GGTCAGAAGC CACCCTTGTC TACGTGGGAT 
AACTCTCCCC TTCGTGTAGG TGGAGGATGG GGAAATTCTG ACGCCAGGTA CACCCCAGGT 
TCCAGTTGGG GTGAGAGCAG CTCAGGGAGA ATAACAAATT GGCTTGTTCT AAAGAACCTC 
ACACCTCAGA TTGACGGCTC AACTCTGCGT ACTCTGTGCA TGCAGCATGG CCCACTGATA 
ACATTCCACC TGAACCTCCC ACATGGAAAT GCTTTGGTCC GTTACAGTTC AAAAGAAGAG 
GTAGTGAAGG CACAAAAGTC TCTGCACATG TGTGTATTGG GGAACACTAC TATTCTTGCT 
GAGTTTGCCA GTGAAGAGGA GATCAGCCGT TTCTTTGCAC AAAGTCAGTC TCTGACCCCT 
TCTCCCGGCT GGCAGTCTCT CGGGTCCAGC CAGAGCCGGC TGGGCTCCCT CGACTGTTCC 
CACTCGTTCT CCAGCCGGAC CGATCTCAAT CACTGGAATG GTGCTGGGCT GTCGGGAACT 
AACTGTGGAG ACCTTCACGG CACTTCACTC TGGGGGACCC CACATTATTC CACAAGCCTG 
TGGGGTCCTC CCAGCAGCAG CGACCCCCGG GGAATTAGCA GCCCATCCCC CATTAATGCT 
TTTCTTTCTG TTGACCACCT GGGTGGGGGT GGAGAGTCCA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_005621402.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005621459.1 Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X5, mRNA.

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CloneID	OCe07959
Clone ID Related Accession (Same CDS sequence)	XM_005621402.2 , XM_005621402.3
Accession Version	XM_005621402.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5805bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	trinucleotide repeat-containing gene 6A protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005621402.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACTAG TGGCAGAGCC AGGCCTAGAA GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG AGAATTGGAA GCTAAAGCTA CCAAAGACGT AGAAAGGACT 
CTTAGCAGGG ATTTAGTGCA AGAAGAAGAA CAGTTGATGG AAGAAAAGAA AAAGAAAAAA 
GACGACAAGA AAAAGAAGGA AGCTGCTCAA AAGAAGGCCA CTGAACAAAA AATCAAAGTG 
CCAGAACAGA TAAAGCCCAG TGTAAGCCAG CCTCAGCCTG CCAACTCTAA TAACGGCACT 
TCCACAGCAA CCAGCACTAA TAATAATGCC AAGCGAGCCA CAGCCAACAA TCCGCAGCAG 
CAGCAGCAGC CACAGCCGCC ACAGCAGCCA CAGCAGCCGC CGCAGCAGCC ACCGCAGCCG 
CCACAGCAGC AGCCGCAGCC AGCACAGGCC TTGCCTCGGT ATCCTCGGGA AGTACCACCA 
CGATTTCGCC ACCAGGAACA CAAACAGCTT CTAAAGAGGG GTCAGCATTT TCCTGTCATA 
GCAGCAAACC TGGGATCTGC TGTTAAAGTG TTAAGCAGCC AGTCAGAAAG CAGTGCTTTA 
ACAAATCAAC AGCCACAAAA TAACGGAGAG GTGCAGAACA GCAAAAACCA GTCAGATATA 
AACCACAGTA CTTCAGGATC CCATTATGAA AATTCACAGC GGGGACCTGT GTCTTCTCCG 
AGTGACTCTA GCACAAATTG TAAGAATGCT GTTGTAAACG ACTCCCCTGA GACAGAAGCA 
TGGCCCTCAG TCCCTGGCAG TGATCCAGAG TTGGCTTCAG AATGTATGGA TGCTGATTCT 
GCCTCCAGTT CTGAATCAGA GAGAAACATC ACTATCATGG CTTCAGGGAG CACAGGTGGT 
GAAAAAGATG GCCTTCGGAA TAGCACTGGA CTTGGTTCCC AAAACAAGTT TGTGGTTGGT 
AGCAGCAGCA ATAATGTGGG CCATGGAAGT AGTCCTGGGC CATGGGGTTT TTCCCATGGA 
GCCATAATAA GCACATGTCA GGTCTCTGTG GATGCTCCTG AAAGCAAATC AGAAAGTAGC 
AACAATAGAA TGAATGCTTG GGGCACTGTA AGTTCTTCAT CAAATGGAGG GTTAAATCCA 
AGCACTTTGA ATTCAGCTAG CAACCATGGT GCCTGGCCAG TATTAGACAA CAACGGACTT 
GCCCTCAAAG GGCCTGTAGG GAGCGGTAGT TCTGGCATCA ATATTCAGTG TAGTACCATA 
GGCCAGATGC CTAACAATCA GAGTATTAAC TCTAAAGTGG GTGGTTCTTC CACCCATGGT 
ACCTGGGGAA GCCTTCAGGA AACTTGTGAA TCTGAAGTAA GTGGTACACA GAAGGTTTCA 
TTCAGTGGTC AACCTCAGAA TATTACCACT GAGATGACTG GACCAAATAA CACTACTAAC 
TTTATGACCT CTAGTTTACC AAACTCCGGT TCAGTACAGA ATAACGAACT GCCTAGTAAT 
ACAGGGGCCT GGCGTGTGAG CACAATGAAT CATCCTCAGA TACAGGCTCC ATCGGTTATG 
AATGGCACTT CCCTTTCTCA TCTTAGCAAT GGAGAGCCAA AAAGTGGAGG CTCTTACGGT 
ACTACGTGGG GTGCCTATGG TTCTAATTAC TCTGGAGACA AATGTTCGAG CCCTAACGGC 
CAAGCTAATG GTGACACTGT GAATGCAACT CTAATGCAGC CTGGCATAAA TGGGCCTATG 
GGCACTAACT TTCAAGTGAA CACAAATAAA GGAGGAGGTG TGTGGGAGTC TGGGGCAACG 
AATTCCCAGA ATGCATCGTG GGGAAGTGGA AATGGCGCAA ATTCTGGAGG AAGTCGACGA 
GGATGGGGAA CTCCTGCACA AAACACTGGC ACTAATATAC CCAGCGTGGA GTGGAGCAAA 
CTGCCTAGCA ATCAGCATTC CAATGACAGT GCAAATGGCA ATGGTAAGAA GTTTACAAAT 
GGATGGAAAT CTACTGAGGA AGAGGATCAG GCTTCTGCCG CATCTCAGAC AACTGAGCAG 
AACAGTGTGT GGGCCAAAAC AGGAGGTACA GTGGAGAGTG AAGGTAGTAC AGAAAGCACT 
GGACGCCTCG AGGAGAAAGC CACTGGGGAA AATCAGAGTA GAGATCGGAG AAAAATTGAT 
CAGCACACAT TACTCCAAAG CATCGTAAAC AGAACTGACT TGGATCCACG TGTCCTCTCC 
AACTCTGGTT GGGGACAGAC TCCTATTAAG CAGAATACTG CCTGGGATAC GGAAACATCA 
CCTAGAGGGG AAAGAAAGAC TGACAATGGG ACAGAGGCCT GGGGAAGCTC TGCAACACAG 
ACTTTTAACT CAGGGGCATG TACAGATAAG ACTAGCCCTA CTAGTAATGA TACCTCATCT 
GTATCGGGGT GGGGAGATCC CAAACCTGCT CTGAGGTGGG GAGATTCCAA AGGCTCAAGC 
TGCCAGGGGG GATGGGAAGA TGATGCTGCT GCTACAGGAA TGGTCAAGAG CAATCAGTGG 
GGGAACAGCA AAGAAGAGAA GTCCGCATGG AATGATTCGC AAAAGAGCAA ACAGGGATGG 
GGTGATGGAC AGAAATCAAA CCAAGGGTGG TCCGTTTCTG CCAGTGATAA CTGGGGAGAA 
ACGTCAAGGA GTAACCATTG GGGTGAGGCT AATAAGAAAT CTAGCTCAGG AGGTAGTGAC 
AGTGACAGGT CTGTTTCTGG TTGGAATGAA CTTGGTAAAA CTAGTTCTTT TACTTGGGGA 
AATAACATAA ATCCAAATAA TTCGTCAGGA TGGGATGAAT CTTCTAAACC TAATCCTTCC 
CAGGGATGGG GAGATCCTCC AAAGTCTAAT CAGTCTCTAG GTTGGGGAGA TTCGTCAAAG 
CCAGTCACTT CTCCAGACTG GAACAAGCAA CAAGACATTG TCGGGTCTTG GGGAATCCCA 
CCAGCAACAG GCAAACCTCC TGGTACAGGC TGGCTGGGAG GACCTATACC AGCCCCAACA 
AAAGAAGAAG AGCCCACAGG CTGGGAGGAA CCATCCCCAG AATCCATACG TCGCAAAATG 
GAGATCGATG ACGGAACTTC AGCTTGGGGA GATCCGAGCA AATACAACTA CAAAAATGTG 
AACATGTGGA ACAAAAATGT CCCAAATGGC AGCAGCCGTT CAGACCAGCA AGCACAGGTA 
CACCAGTTGC TACCATCTGC AAGTGCCATC TCAAACAAAG AGGCAGGCAG TGGCTCTGGC 
TGGGGTGAGC CCTGGGGGGA GCCTTCTACT CCAGCCACAA CTGTGGATAA TGGTACTTCA 
GCATGGGGTA AACCCATAGA CAGTGGTCCC AGCTGGGGGG AGCCCATTGC TGCAGCATCC 
AACACATCCA CGTGGGGCTC CAGCTCTGTT GGTCCTCAAG CATTAAGCAA ATCTGGGCCA 
AAATCTATGC AAGATGGCTG GTGTGGTGAT GATATGCCAT TGCCTGGAAA TCGCCCCACT 
GGCTGGGAAG AGGAAGAGGA TGTAGAGATT GGAATGTGGA ATAGTAATTC ATCTCAAGAG 
CTTAACTCAT CTTTAAATTG GCCTCCATAT ACAAAGAAAA TGTCATCGAA GGGTCTGAGT 
GGCAAAAAAA GGAGAAGGGA AAGGGGAACC ATGAAAGGTG GAAACAAACA AGAAGAAGCA 
TGGATAAATC CATTCGTTAA ACAGTTTTCA AATATCAGTT TTTCGAGAGA CTCACCCGAA 
GAAAATGTAC AGAGCAATAA GATGGATCTT TCCGGAGGAA TGTTACAGGA CAAACGAATG 
GAGATAGATA AACATAGCCT AAATATTGGT GATTACAATC GAACGGTCGG GAAAGGCCCT 
GGTTCTCGGC CTCAGATTTC CAAAGAGTCT TCCATGGAGC GCAGCCCTTA CTTTGATAAG 
AATGGCAATC CCAGTATGTT TGGTGTTGGA AACACAGCAG CACAACCCCG GGGCATGCAG 
CAGCCTCCAG CACAACCTCT TAGTTCATCT CAGCCTAATC TTCGTGCTCA AGTGCCTCCT 
CCATTACTCT CCCCTCAGGT TCCAGTTTCA TTGCTGAAGT ATGCACCAAA CAACGGTGGC 
CTGAATCCGC TCTTTGGCCC TCAACAGGTA GCCATGCTGA ACCAGCTATC CCAACTAAAC 
CAGCTTTCTC AGATCTCCCA GTTACAGAGA TTGTTAGCGC AGCAGCAAAG GGCGCAGAGT 
CAGAGAAGCG TGCCTTCTGG GAACCGGCAG CAGCAAGACC AGCAGGGTCG ACCTCTTAGC 
GTGCAGCAGC AGATGATGCA GCAGTCCCGG CAACTCGATC CCAGCCTGTT GGTGAAGCAG 
CAGACCCCGC CATCTCAGCA GCAGCCACTC CATCAGCCAG CCATGAAATC CTTCCTCGAG 
AATGTCATGC CCCACACTAC ACCTGAGCTG CAGAAAGGGC CATCACCAAT AAATGCTTTC 
AGCAACTTCC CGATAGGCTT GAACTCAAAC TTGAATGTAA ATATGGATAT GAACAGTATT 
AAAGAGCCAC AGTCAAGACT AAGGAAGTGG ACTACAGTGG ACAGCATTTC TGTGAACACA 
TCTTTGGATC AAAACTCCAG CAAACATGGT GCTATTTCAA GTGGTTTCAG GCTGGAAGAG 
TCTCCATTTG TTCCCTATGA CTTTATGAAC AGCAGTACTT CTCCAGCCAG CCCTCCGGGT 
TCAATAGGAG ATGGCTGGCC ACGTGCCAAA TCGCCTAACG GCTCTAGCAG TGTTAACTGG 
CCACCAGAAT TTCGCCCTGG TGAACCATGG AAAGGTTATC CAAACATTGA CCCTGAAACT 
GACCCTTACG TCACTCCTGG CAGTGTCATA AACAATCTTT CAATTAATAC TGTGCGGGAA 
GTTGACCACC TCAGGGACAG GAACAGTGGG TCATCCTCAT CCTTGAACAC CACGCTGCCT 
TCAACTAGTG CCTGGTCATC CATTCGTGCC TCCAACTACA ACGTTCCCCT CAGCAGTACA 
GCACAAAGCA CTTCAGCCAG AAATAGTGAT TCCAAATTGA CATGGTCTCC TGGTTCAGTT 
ACAAACACCT CTCTGGCTCA TGAGCTGTGG AAGGTCCCTT TACCACCTAA AAACATCACT 
GCTCCGTCCC GCCCACCTCC GGGACTCACT GGTCAGAAGC CACCCTTGTC TACGTGGGAT 
AACTCTCCCC TTCGTGTAGG TGGAGGATGG GGAAATTCTG ACGCCAGGTA CACCCCAGGT 
TCCAGTTGGG GTGAGAGCAG CTCAGGGAGA ATAACAAATT GGCTTGTTCT AAAGAACCTC 
ACACCTCAGA TTGACGGCTC AACTCTGCGT ACTCTGTGCA TGCAGCATGG CCCACTGATA 
ACATTCCACC TGAACCTCCC ACATGGAAAT GCTTTGGTCC GTTACAGTTC AAAAGAAGAG 
GTAGTGAAGG CACAAAAGTC TCTGCACATG TGTGTATTGG GGAACACTAC TATTCTTGCT 
GAGTTTGCCA GTGAAGAGGA GATCAGCCGT TTCTTTGCAC AAAGTCAGTC TCTGACCCCT 
TCTCCCGGCT GGCAGTCTCT CGGGTCCAGC CAGAGCCGGC TGGGCTCCCT CGACTGTTCC 
CACTCGTTCT CCAGCCGGAC CGATCTCAAT CACTGGAATG GTGCTGGGCT GTCGGGAACT 
AACTGTGGAG ACCTTCACGG CACTTCACTC TGGGGGACCC CACATTATTC CACAAGCCTG 
TGGGGTCCTC CCAGCAGCAG CGACCCCCGG GGAATTAGCA GCCCATCCCC CATTAATGCT 
TTTCTTTCTG TTGACCACCT GGGTGGGGGT GGAGAGTCCA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005621459.1
CDS	276..6080
Translation	MQLVAEPGLEARCPGSQDIPLPEEWNRELEAKATKDVERTLSRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNPQQQQQPQPPQQPQQPPQQPPQPPQQQPQPAQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLSSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSPSDSSTNCKNAVVNDSPETEAWPSVPGSDPELASECMDADSASSSESERNITIMASGSTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSPGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLDNNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVGGSSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVMNGTSLSHLSNGEPKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGINGPMGTNFQVNTNKGGGVWESGATNSQNASWGSGNGANSGGSRRGWGTPAQNTGTNIPSVEWSKLPSNQHSNDSANGNGKKFTNGWKSTEEEDQASAASQTTEQNSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACTDKTSPTSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDAAATGMVKSNQWGNSKEEKSAWNDSQKSKQGWGDGQKSNQGWSVSASDNWGETSRSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVTSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPTKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSASAISNKEAGSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASNTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGTMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERSPYFDKNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPSLLVKQQTPPSQQQPLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_005621402.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005621402.3

ORF Insert Sequence:

ATGCAACTAG TGGCAGAGCC AGGCCTAGAA GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG AGAATTGGAA GCTAAAGCTA CCAAAGACGT AGAAAGGACT 
CTTAGCAGGG ATTTAGTGCA AGAAGAAGAA CAGTTGATGG AAGAAAAGAA AAAGAAAAAA 
GACGACAAGA AAAAGAAGGA AGCTGCTCAA AAGAAGGCCA CTGAACAAAA AATCAAAGTG 
CCAGAACAGA TAAAGCCCAG TGTAAGCCAG CCTCAGCCTG CCAACTCTAA TAACGGCACT 
TCCACAGCAA CCAGCACTAA TAATAATGCC AAGCGAGCCA CAGCCAACAA TCCGCAGCAG 
CAGCAGCAGC CACAGCCGCC ACAGCAGCCA CAGCAGCCGC CGCAGCAGCC ACCGCAGCCG 
CCACAGCAGC AGCCGCAGCC AGCACAGGCC TTGCCTCGGT ATCCTCGGGA AGTACCACCA 
CGATTTCGCC ACCAGGAACA CAAACAGCTT CTAAAGAGGG GTCAGCATTT TCCTGTCATA 
GCAGCAAACC TGGGATCTGC TGTTAAAGTG TTAAGCAGCC AGTCAGAAAG CAGTGCTTTA 
ACAAATCAAC AGCCACAAAA TAACGGAGAG GTGCAGAACA GCAAAAACCA GTCAGATATA 
AACCACAGTA CTTCAGGATC CCATTATGAA AATTCACAGC GGGGACCTGT GTCTTCTCCG 
AGTGACTCTA GCACAAATTG TAAGAATGCT GTTGTAAACG ACTCCCCTGA GACAGAAGCA 
TGGCCCTCAG TCCCTGGCAG TGATCCAGAG TTGGCTTCAG AATGTATGGA TGCTGATTCT 
GCCTCCAGTT CTGAATCAGA GAGAAACATC ACTATCATGG CTTCAGGGAG CACAGGTGGT 
GAAAAAGATG GCCTTCGGAA TAGCACTGGA CTTGGTTCCC AAAACAAGTT TGTGGTTGGT 
AGCAGCAGCA ATAATGTGGG CCATGGAAGT AGTCCTGGGC CATGGGGTTT TTCCCATGGA 
GCCATAATAA GCACATGTCA GGTCTCTGTG GATGCTCCTG AAAGCAAATC AGAAAGTAGC 
AACAATAGAA TGAATGCTTG GGGCACTGTA AGTTCTTCAT CAAATGGAGG GTTAAATCCA 
AGCACTTTGA ATTCAGCTAG CAACCATGGT GCCTGGCCAG TATTAGACAA CAACGGACTT 
GCCCTCAAAG GGCCTGTAGG GAGCGGTAGT TCTGGCATCA ATATTCAGTG TAGTACCATA 
GGCCAGATGC CTAACAATCA GAGTATTAAC TCTAAAGTGG GTGGTTCTTC CACCCATGGT 
ACCTGGGGAA GCCTTCAGGA AACTTGTGAA TCTGAAGTAA GTGGTACACA GAAGGTTTCA 
TTCAGTGGTC AACCTCAGAA TATTACCACT GAGATGACTG GACCAAATAA CACTACTAAC 
TTTATGACCT CTAGTTTACC AAACTCCGGT TCAGTACAGA ATAACGAACT GCCTAGTAAT 
ACAGGGGCCT GGCGTGTGAG CACAATGAAT CATCCTCAGA TACAGGCTCC ATCGGTTATG 
AATGGCACTT CCCTTTCTCA TCTTAGCAAT GGAGAGCCAA AAAGTGGAGG CTCTTACGGT 
ACTACGTGGG GTGCCTATGG TTCTAATTAC TCTGGAGACA AATGTTCGAG CCCTAACGGC 
CAAGCTAATG GTGACACTGT GAATGCAACT CTAATGCAGC CTGGCATAAA TGGGCCTATG 
GGCACTAACT TTCAAGTGAA CACAAATAAA GGAGGAGGTG TGTGGGAGTC TGGGGCAACG 
AATTCCCAGA ATGCATCGTG GGGAAGTGGA AATGGCGCAA ATTCTGGAGG AAGTCGACGA 
GGATGGGGAA CTCCTGCACA AAACACTGGC ACTAATATAC CCAGCGTGGA GTGGAGCAAA 
CTGCCTAGCA ATCAGCATTC CAATGACAGT GCAAATGGCA ATGGTAAGAA GTTTACAAAT 
GGATGGAAAT CTACTGAGGA AGAGGATCAG GCTTCTGCCG CATCTCAGAC AACTGAGCAG 
AACAGTGTGT GGGCCAAAAC AGGAGGTACA GTGGAGAGTG AAGGTAGTAC AGAAAGCACT 
GGACGCCTCG AGGAGAAAGC CACTGGGGAA AATCAGAGTA GAGATCGGAG AAAAATTGAT 
CAGCACACAT TACTCCAAAG CATCGTAAAC AGAACTGACT TGGATCCACG TGTCCTCTCC 
AACTCTGGTT GGGGACAGAC TCCTATTAAG CAGAATACTG CCTGGGATAC GGAAACATCA 
CCTAGAGGGG AAAGAAAGAC TGACAATGGG ACAGAGGCCT GGGGAAGCTC TGCAACACAG 
ACTTTTAACT CAGGGGCATG TACAGATAAG ACTAGCCCTA CTAGTAATGA TACCTCATCT 
GTATCGGGGT GGGGAGATCC CAAACCTGCT CTGAGGTGGG GAGATTCCAA AGGCTCAAGC 
TGCCAGGGGG GATGGGAAGA TGATGCTGCT GCTACAGGAA TGGTCAAGAG CAATCAGTGG 
GGGAACAGCA AAGAAGAGAA GTCCGCATGG AATGATTCGC AAAAGAGCAA ACAGGGATGG 
GGTGATGGAC AGAAATCAAA CCAAGGGTGG TCCGTTTCTG CCAGTGATAA CTGGGGAGAA 
ACGTCAAGGA GTAACCATTG GGGTGAGGCT AATAAGAAAT CTAGCTCAGG AGGTAGTGAC 
AGTGACAGGT CTGTTTCTGG TTGGAATGAA CTTGGTAAAA CTAGTTCTTT TACTTGGGGA 
AATAACATAA ATCCAAATAA TTCGTCAGGA TGGGATGAAT CTTCTAAACC TAATCCTTCC 
CAGGGATGGG GAGATCCTCC AAAGTCTAAT CAGTCTCTAG GTTGGGGAGA TTCGTCAAAG 
CCAGTCACTT CTCCAGACTG GAACAAGCAA CAAGACATTG TCGGGTCTTG GGGAATCCCA 
CCAGCAACAG GCAAACCTCC TGGTACAGGC TGGCTGGGAG GACCTATACC AGCCCCAACA 
AAAGAAGAAG AGCCCACAGG CTGGGAGGAA CCATCCCCAG AATCCATACG TCGCAAAATG 
GAGATCGATG ACGGAACTTC AGCTTGGGGA GATCCGAGCA AATACAACTA CAAAAATGTG 
AACATGTGGA ACAAAAATGT CCCAAATGGC AGCAGCCGTT CAGACCAGCA AGCACAGGTA 
CACCAGTTGC TACCATCTGC AAGTGCCATC TCAAACAAAG AGGCAGGCAG TGGCTCTGGC 
TGGGGTGAGC CCTGGGGGGA GCCTTCTACT CCAGCCACAA CTGTGGATAA TGGTACTTCA 
GCATGGGGTA AACCCATAGA CAGTGGTCCC AGCTGGGGGG AGCCCATTGC TGCAGCATCC 
AACACATCCA CGTGGGGCTC CAGCTCTGTT GGTCCTCAAG CATTAAGCAA ATCTGGGCCA 
AAATCTATGC AAGATGGCTG GTGTGGTGAT GATATGCCAT TGCCTGGAAA TCGCCCCACT 
GGCTGGGAAG AGGAAGAGGA TGTAGAGATT GGAATGTGGA ATAGTAATTC ATCTCAAGAG 
CTTAACTCAT CTTTAAATTG GCCTCCATAT ACAAAGAAAA TGTCATCGAA GGGTCTGAGT 
GGCAAAAAAA GGAGAAGGGA AAGGGGAACC ATGAAAGGTG GAAACAAACA AGAAGAAGCA 
TGGATAAATC CATTCGTTAA ACAGTTTTCA AATATCAGTT TTTCGAGAGA CTCACCCGAA 
GAAAATGTAC AGAGCAATAA GATGGATCTT TCCGGAGGAA TGTTACAGGA CAAACGAATG 
GAGATAGATA AACATAGCCT AAATATTGGT GATTACAATC GAACGGTCGG GAAAGGCCCT 
GGTTCTCGGC CTCAGATTTC CAAAGAGTCT TCCATGGAGC GCAGCCCTTA CTTTGATAAG 
AATGGCAATC CCAGTATGTT TGGTGTTGGA AACACAGCAG CACAACCCCG GGGCATGCAG 
CAGCCTCCAG CACAACCTCT TAGTTCATCT CAGCCTAATC TTCGTGCTCA AGTGCCTCCT 
CCATTACTCT CCCCTCAGGT TCCAGTTTCA TTGCTGAAGT ATGCACCAAA CAACGGTGGC 
CTGAATCCGC TCTTTGGCCC TCAACAGGTA GCCATGCTGA ACCAGCTATC CCAACTAAAC 
CAGCTTTCTC AGATCTCCCA GTTACAGAGA TTGTTAGCGC AGCAGCAAAG GGCGCAGAGT 
CAGAGAAGCG TGCCTTCTGG GAACCGGCAG CAGCAAGACC AGCAGGGTCG ACCTCTTAGC 
GTGCAGCAGC AGATGATGCA GCAGTCCCGG CAACTCGATC CCAGCCTGTT GGTGAAGCAG 
CAGACCCCGC CATCTCAGCA GCAGCCACTC CATCAGCCAG CCATGAAATC CTTCCTCGAG 
AATGTCATGC CCCACACTAC ACCTGAGCTG CAGAAAGGGC CATCACCAAT AAATGCTTTC 
AGCAACTTCC CGATAGGCTT GAACTCAAAC TTGAATGTAA ATATGGATAT GAACAGTATT 
AAAGAGCCAC AGTCAAGACT AAGGAAGTGG ACTACAGTGG ACAGCATTTC TGTGAACACA 
TCTTTGGATC AAAACTCCAG CAAACATGGT GCTATTTCAA GTGGTTTCAG GCTGGAAGAG 
TCTCCATTTG TTCCCTATGA CTTTATGAAC AGCAGTACTT CTCCAGCCAG CCCTCCGGGT 
TCAATAGGAG ATGGCTGGCC ACGTGCCAAA TCGCCTAACG GCTCTAGCAG TGTTAACTGG 
CCACCAGAAT TTCGCCCTGG TGAACCATGG AAAGGTTATC CAAACATTGA CCCTGAAACT 
GACCCTTACG TCACTCCTGG CAGTGTCATA AACAATCTTT CAATTAATAC TGTGCGGGAA 
GTTGACCACC TCAGGGACAG GAACAGTGGG TCATCCTCAT CCTTGAACAC CACGCTGCCT 
TCAACTAGTG CCTGGTCATC CATTCGTGCC TCCAACTACA ACGTTCCCCT CAGCAGTACA 
GCACAAAGCA CTTCAGCCAG AAATAGTGAT TCCAAATTGA CATGGTCTCC TGGTTCAGTT 
ACAAACACCT CTCTGGCTCA TGAGCTGTGG AAGGTCCCTT TACCACCTAA AAACATCACT 
GCTCCGTCCC GCCCACCTCC GGGACTCACT GGTCAGAAGC CACCCTTGTC TACGTGGGAT 
AACTCTCCCC TTCGTGTAGG TGGAGGATGG GGAAATTCTG ACGCCAGGTA CACCCCAGGT 
TCCAGTTGGG GTGAGAGCAG CTCAGGGAGA ATAACAAATT GGCTTGTTCT AAAGAACCTC 
ACACCTCAGA TTGACGGCTC AACTCTGCGT ACTCTGTGCA TGCAGCATGG CCCACTGATA 
ACATTCCACC TGAACCTCCC ACATGGAAAT GCTTTGGTCC GTTACAGTTC AAAAGAAGAG 
GTAGTGAAGG CACAAAAGTC TCTGCACATG TGTGTATTGG GGAACACTAC TATTCTTGCT 
GAGTTTGCCA GTGAAGAGGA GATCAGCCGT TTCTTTGCAC AAAGTCAGTC TCTGACCCCT 
TCTCCCGGCT GGCAGTCTCT CGGGTCCAGC CAGAGCCGGC TGGGCTCCCT CGACTGTTCC 
CACTCGTTCT CCAGCCGGAC CGATCTCAAT CACTGGAATG GTGCTGGGCT GTCGGGAACT 
AACTGTGGAG ACCTTCACGG CACTTCACTC TGGGGGACCC CACATTATTC CACAAGCCTG 
TGGGGTCCTC CCAGCAGCAG CGACCCCCGG GGAATTAGCA GCCCATCCCC CATTAATGCT 
TTTCTTTCTG TTGACCACCT GGGTGGGGGT GGAGAGTCCA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_005621403.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005621460.1 Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X5, mRNA.

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CloneID	OCe07960
Clone ID Related Accession (Same CDS sequence)	XM_005621403.2 , XM_547086.5 , XM_005621403.3
Accession Version	XM_005621403.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	trinucleotide repeat-containing gene 6A protein isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005621403.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA AGAAAAAGAA AAAAGACGAC AAGAAAAAGA AGGAAGCTGC TCAAAAGAAG 
GCCACTGAAC AAAAAATCAA AGTGCCAGAA CAGATAAAGC CCAGTGTAAG CCAGCCTCAG 
CCTGCCAACT CTAATAACGG CACTTCCACA GCAACCAGCA CTAATAATAA TGCCAAGCGA 
GCCACAGCCA ACAATCCGCA GCAGCAGCAG CAGCCACAGC CGCCACAGCA GCCACAGCAG 
CCGCCGCAGC AGCCACCGCA GCCGCCACAG CAGCAGCCGC AGCCAGCACA GGCCTTGCCT 
CGGTATCCTC GGGAAGTACC ACCACGATTT CGCCACCAGG AACACAAACA GCTTCTAAAG 
AGGGGTCAGC ATTTTCCTGT CATAGCAGCA AACCTGGGAT CTGCTGTTAA AGTGTTAAGC 
AGCCAGTCAG AAAGCAGTGC TTTAACAAAT CAACAGCCAC AAAATAACGG AGAGGTGCAG 
AACAGCAAAA ACCAGTCAGA TATAAACCAC AGTACTTCAG GATCCCATTA TGAAAATTCA 
CAGCGGGGAC CTGTGTCTTC TCCGAGTGAC TCTAGCACAA ATTGTAAGAA TGCTGTTGTA 
AACGACTCCC CTGAGACAGA AGCATGGCCC TCAGTCCCTG GCAGTGATCC AGAGTTGGCT 
TCAGAATGTA TGGATGCTGA TTCTGCCTCC AGTTCTGAAT CAGAGAGAAA CATCACTATC 
ATGGCTTCAG GGAGCACAGG TGGTGAAAAA GATGGCCTTC GGAATAGCAC TGGACTTGGT 
TCCCAAAACA AGTTTGTGGT TGGTAGCAGC AGCAATAATG TGGGCCATGG AAGTAGTCCT 
GGGCCATGGG GTTTTTCCCA TGGAGCCATA ATAAGCACAT GTCAGGTCTC TGTGGATGCT 
CCTGAAAGCA AATCAGAAAG TAGCAACAAT AGAATGAATG CTTGGGGCAC TGTAAGTTCT 
TCATCAAATG GAGGGTTAAA TCCAAGCACT TTGAATTCAG CTAGCAACCA TGGTGCCTGG 
CCAGTATTAG ACAACAACGG ACTTGCCCTC AAAGGGCCTG TAGGGAGCGG TAGTTCTGGC 
ATCAATATTC AGTGTAGTAC CATAGGCCAG ATGCCTAACA ATCAGAGTAT TAACTCTAAA 
GTGGGTGGTT CTTCCACCCA TGGTACCTGG GGAAGCCTTC AGGAAACTTG TGAATCTGAA 
GTAAGTGGTA CACAGAAGGT TTCATTCAGT GGTCAACCTC AGAATATTAC CACTGAGATG 
ACTGGACCAA ATAACACTAC TAACTTTATG ACCTCTAGTT TACCAAACTC CGGTTCAGTA 
CAGAATAACG AACTGCCTAG TAATACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT 
CAGATACAGG CTCCATCGGT TATGAATGGC ACTTCCCTTT CTCATCTTAG CAATGGAGAG 
CCAAAAAGTG GAGGCTCTTA CGGTACTACG TGGGGTGCCT ATGGTTCTAA TTACTCTGGA 
GACAAATGTT CGAGCCCTAA CGGCCAAGCT AATGGTGACA CTGTGAATGC AACTCTAATG 
CAGCCTGGCA TAAATGGGCC TATGGGCACT AACTTTCAAG TGAACACAAA TAAAGGAGGA 
GGTGTGTGGG AGTCTGGGGC AACGAATTCC CAGAATGCAT CGTGGGGAAG TGGAAATGGC 
GCAAATTCTG GAGGAAGTCG ACGAGGATGG GGAACTCCTG CACAAAACAC TGGCACTAAT 
ATACCCAGCG TGGAGTGGAG CAAACTGCCT AGCAATCAGC ATTCCAATGA CAGTGCAAAT 
GGCAATGGTA AGAAGTTTAC AAATGGATGG AAATCTACTG AGGAAGAGGA TCAGGCTTCT 
GCCGCATCTC AGACAACTGA GCAGAACAGT GTGTGGGCCA AAACAGGAGG TACAGTGGAG 
AGTGAAGGTA GTACAGAAAG CACTGGACGC CTCGAGGAGA AAGCCACTGG GGAAAATCAG 
AGTAGAGATC GGAGAAAAAT TGATCAGCAC ACATTACTCC AAAGCATCGT AAACAGAACT 
GACTTGGATC CACGTGTCCT CTCCAACTCT GGTTGGGGAC AGACTCCTAT TAAGCAGAAT 
ACTGCCTGGG ATACGGAAAC ATCACCTAGA GGGGAAAGAA AGACTGACAA TGGGACAGAG 
GCCTGGGGAA GCTCTGCAAC ACAGACTTTT AACTCAGGGG CATGTACAGA TAAGACTAGC 
CCTACTAGTA ATGATACCTC ATCTGTATCG GGGTGGGGAG ATCCCAAACC TGCTCTGAGG 
TGGGGAGATT CCAAAGGCTC AAGCTGCCAG GGGGGATGGG AAGATGATGC TGCTGCTACA 
GGAATGGTCA AGAGCAATCA GTGGGGGAAC AGCAAAGAAG AGAAGTCCGC ATGGAATGAT 
TCGCAAAAGA GCAAACAGGG ATGGGGTGAT GGACAGAAAT CAAACCAAGG GTGGTCCGTT 
TCTGCCAGTG ATAACTGGGG AGAAACGTCA AGGAGTAACC ATTGGGGTGA GGCTAATAAG 
AAATCTAGCT CAGGAGGTAG TGACAGTGAC AGGTCTGTTT CTGGTTGGAA TGAACTTGGT 
AAAACTAGTT CTTTTACTTG GGGAAATAAC ATAAATCCAA ATAATTCGTC AGGATGGGAT 
GAATCTTCTA AACCTAATCC TTCCCAGGGA TGGGGAGATC CTCCAAAGTC TAATCAGTCT 
CTAGGTTGGG GAGATTCGTC AAAGCCAGTC ACTTCTCCAG ACTGGAACAA GCAACAAGAC 
ATTGTCGGGT CTTGGGGAAT CCCACCAGCA ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG 
GGAGGACCTA TACCAGCCCC AACAAAAGAA GAAGAGCCCA CAGGCTGGGA GGAACCATCC 
CCAGAATCCA TACGTCGCAA AATGGAGATC GATGACGGAA CTTCAGCTTG GGGAGATCCG 
AGCAAATACA ACTACAAAAA TGTGAACATG TGGAACAAAA ATGTCCCAAA TGGCAGCAGC 
CGTTCAGACC AGCAAGCACA GGTACACCAG TTGCTACCAT CTGCAAGTGC CATCTCAAAC 
AAAGAGGCAG GCAGTGGCTC TGGCTGGGGT GAGCCCTGGG GGGAGCCTTC TACTCCAGCC 
ACAACTGTGG ATAATGGTAC TTCAGCATGG GGTAAACCCA TAGACAGTGG TCCCAGCTGG 
GGGGAGCCCA TTGCTGCAGC ATCCAACACA TCCACGTGGG GCTCCAGCTC TGTTGGTCCT 
CAAGCATTAA GCAAATCTGG GCCAAAATCT ATGCAAGATG GCTGGTGTGG TGATGATATG 
CCATTGCCTG GAAATCGCCC CACTGGCTGG GAAGAGGAAG AGGATGTAGA GATTGGAATG 
TGGAATAGTA ATTCATCTCA AGAGCTTAAC TCATCTTTAA ATTGGCCTCC ATATACAAAG 
AAAATGTCAT CGAAGGGTCT GAGTGGCAAA AAAAGGAGAA GGGAAAGGGG AACCATGAAA 
GGTGGAAACA AACAAGAAGA AGCATGGATA AATCCATTCG TTAAACAGTT TTCAAATATC 
AGTTTTTCGA GAGACTCACC CGAAGAAAAT GTACAGAGCA ATAAGATGGA TCTTTCCGGA 
GGAATGTTAC AGGACAAACG AATGGAGATA GATAAACATA GCCTAAATAT TGGTGATTAC 
AATCGAACGG TCGGGAAAGG CCCTGGTTCT CGGCCTCAGA TTTCCAAAGA GTCTTCCATG 
GAGCGCAGCC CTTACTTTGA TAAGGATGGC ATTGTAGCAG ATGAATCCCA AAACATGCAG 
TTTATGTCCA GTCAAAGCAT GAAGCTTCCC CCTTCAAATA GTGCACTACC TAACCAGGCC 
CTTGGCTCCA TAGCAGGGCT GGGTATGCAA AACTTGAATT CTGTTAGACA GAATGGCAAT 
CCCAGTATGT TTGGTGTTGG AAACACAGCA GCACAACCCC GGGGCATGCA GCAGCCTCCA 
GCACAACCTC TTAGTTCATC TCAGCCTAAT CTTCGTGCTC AAGTGCCTCC TCCATTACTC 
TCCCCTCAGG TTCCAGTTTC ATTGCTGAAG TATGCACCAA ACAACGGTGG CCTGAATCCG 
CTCTTTGGCC CTCAACAGGT AGCCATGCTG AACCAGCTAT CCCAACTAAA CCAGCTTTCT 
CAGATCTCCC AGTTACAGAG ATTGTTAGCG CAGCAGCAAA GGGCGCAGAG TCAGAGAAGC 
GTGCCTTCTG GGAACCGGCA GCAGCAAGAC CAGCAGGGTC GACCTCTTAG CGTGCAGCAG 
CAGATGATGC AGCAGTCCCG GCAACTCGAT CCCAGCCTGT TGGTGAAGCA GCAGACCCCG 
CCATCTCAGC AGCAGCCACT CCATCAGCCA GCCATGAAAT CCTTCCTCGA GAATGTCATG 
CCCCACACTA CACCTGAGCT GCAGAAAGGG CCATCACCAA TAAATGCTTT CAGCAACTTC 
CCGATAGGCT TGAACTCAAA CTTGAATGTA AATATGGATA TGAACAGTAT TAAAGAGCCA 
CAGTCAAGAC TAAGGAAGTG GACTACAGTG GACAGCATTT CTGTGAACAC ATCTTTGGAT 
CAAAACTCCA GCAAACATGG TGCTATTTCA AGTGGTTTCA GGCTGGAAGA GTCTCCATTT 
GTTCCCTATG ACTTTATGAA CAGCAGTACT TCTCCAGCCA GCCCTCCGGG TTCAATAGGA 
GATGGCTGGC CACGTGCCAA ATCGCCTAAC GGCTCTAGCA GTGTTAACTG GCCACCAGAA 
TTTCGCCCTG GTGAACCATG GAAAGGTTAT CCAAACATTG ACCCTGAAAC TGACCCTTAC 
GTCACTCCTG GCAGTGTCAT AAACAATCTT TCAATTAATA CTGTGCGGGA AGTTGACCAC 
CTCAGGGACA GGAACAGTGG GTCATCCTCA TCCTTGAACA CCACGCTGCC TTCAACTAGT 
GCCTGGTCAT CCATTCGTGC CTCCAACTAC AACGTTCCCC TCAGCAGTAC AGCACAAAGC 
ACTTCAGCCA GAAATAGTGA TTCCAAATTG ACATGGTCTC CTGGTTCAGT TACAAACACC 
TCTCTGGCTC ATGAGCTGTG GAAGGTCCCT TTACCACCTA AAAACATCAC TGCTCCGTCC 
CGCCCACCTC CGGGACTCAC TGGTCAGAAG CCACCCTTGT CTACGTGGGA TAACTCTCCC 
CTTCGTGTAG GTGGAGGATG GGGAAATTCT GACGCCAGGT ACACCCCAGG TTCCAGTTGG 
GGTGAGAGCA GCTCAGGGAG AATAACAAAT TGGCTTGTTC TAAAGAACCT CACACCTCAG 
ATTGACGGCT CAACTCTGCG TACTCTGTGC ATGCAGCATG GCCCACTGAT AACATTCCAC 
CTGAACCTCC CACATGGAAA TGCTTTGGTC CGTTACAGTT CAAAAGAAGA GGTAGTGAAG 
GCACAAAAGT CTCTGCACAT GTGTGTATTG GGGAACACTA CTATTCTTGC TGAGTTTGCC 
AGTGAAGAGG AGATCAGCCG TTTCTTTGCA CAAAGTCAGT CTCTGACCCC TTCTCCCGGC 
TGGCAGTCTC TCGGGTCCAG CCAGAGCCGG CTGGGCTCCC TCGACTGTTC CCACTCGTTC 
TCCAGCCGGA CCGATCTCAA TCACTGGAAT GGTGCTGGGC TGTCGGGAAC TAACTGTGGA 
GACCTTCACG GCACTTCACT CTGGGGGACC CCACATTATT CCACAAGCCT GTGGGGTCCT 
CCCAGCAGCA GCGACCCCCG GGGAATTAGC AGCCCATCCC CCATTAATGC TTTTCTTTCT 
GTTGACCACC TGGGTGGGGG TGGAGAGTCC ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005621460.1
CDS	425..6220
Translation	MEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNPQQQQQPQPPQQPQQPPQQPPQPPQQQPQPAQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLSSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSPSDSSTNCKNAVVNDSPETEAWPSVPGSDPELASECMDADSASSSESERNITIMASGSTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSPGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLDNNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVGGSSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVMNGTSLSHLSNGEPKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGINGPMGTNFQVNTNKGGGVWESGATNSQNASWGSGNGANSGGSRRGWGTPAQNTGTNIPSVEWSKLPSNQHSNDSANGNGKKFTNGWKSTEEEDQASAASQTTEQNSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACTDKTSPTSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDAAATGMVKSNQWGNSKEEKSAWNDSQKSKQGWGDGQKSNQGWSVSASDNWGETSRSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVTSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPTKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSASAISNKEAGSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASNTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGTMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERSPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPSLLVKQQTPPSQQQPLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_005621403.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005621403.2

ORF Insert Sequence:

ATGGAAGAAA AGAAAAAGAA AAAAGACGAC AAGAAAAAGA AGGAAGCTGC TCAAAAGAAG 
GCCACTGAAC AAAAAATCAA AGTGCCAGAA CAGATAAAGC CCAGTGTAAG CCAGCCTCAG 
CCTGCCAACT CTAATAACGG CACTTCCACA GCAACCAGCA CTAATAATAA TGCCAAGCGA 
GCCACAGCCA ACAATCCGCA GCAGCAGCAG CAGCCACAGC CGCCACAGCA GCCACAGCAG 
CCGCCGCAGC AGCCACCGCA GCCGCCACAG CAGCAGCCGC AGCCAGCACA GGCCTTGCCT 
CGGTATCCTC GGGAAGTACC ACCACGATTT CGCCACCAGG AACACAAACA GCTTCTAAAG 
AGGGGTCAGC ATTTTCCTGT CATAGCAGCA AACCTGGGAT CTGCTGTTAA AGTGTTAAGC 
AGCCAGTCAG AAAGCAGTGC TTTAACAAAT CAACAGCCAC AAAATAACGG AGAGGTGCAG 
AACAGCAAAA ACCAGTCAGA TATAAACCAC AGTACTTCAG GATCCCATTA TGAAAATTCA 
CAGCGGGGAC CTGTGTCTTC TCCGAGTGAC TCTAGCACAA ATTGTAAGAA TGCTGTTGTA 
AACGACTCCC CTGAGACAGA AGCATGGCCC TCAGTCCCTG GCAGTGATCC AGAGTTGGCT 
TCAGAATGTA TGGATGCTGA TTCTGCCTCC AGTTCTGAAT CAGAGAGAAA CATCACTATC 
ATGGCTTCAG GGAGCACAGG TGGTGAAAAA GATGGCCTTC GGAATAGCAC TGGACTTGGT 
TCCCAAAACA AGTTTGTGGT TGGTAGCAGC AGCAATAATG TGGGCCATGG AAGTAGTCCT 
GGGCCATGGG GTTTTTCCCA TGGAGCCATA ATAAGCACAT GTCAGGTCTC TGTGGATGCT 
CCTGAAAGCA AATCAGAAAG TAGCAACAAT AGAATGAATG CTTGGGGCAC TGTAAGTTCT 
TCATCAAATG GAGGGTTAAA TCCAAGCACT TTGAATTCAG CTAGCAACCA TGGTGCCTGG 
CCAGTATTAG ACAACAACGG ACTTGCCCTC AAAGGGCCTG TAGGGAGCGG TAGTTCTGGC 
ATCAATATTC AGTGTAGTAC CATAGGCCAG ATGCCTAACA ATCAGAGTAT TAACTCTAAA 
GTGGGTGGTT CTTCCACCCA TGGTACCTGG GGAAGCCTTC AGGAAACTTG TGAATCTGAA 
GTAAGTGGTA CACAGAAGGT TTCATTCAGT GGTCAACCTC AGAATATTAC CACTGAGATG 
ACTGGACCAA ATAACACTAC TAACTTTATG ACCTCTAGTT TACCAAACTC CGGTTCAGTA 
CAGAATAACG AACTGCCTAG TAATACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT 
CAGATACAGG CTCCATCGGT TATGAATGGC ACTTCCCTTT CTCATCTTAG CAATGGAGAG 
CCAAAAAGTG GAGGCTCTTA CGGTACTACG TGGGGTGCCT ATGGTTCTAA TTACTCTGGA 
GACAAATGTT CGAGCCCTAA CGGCCAAGCT AATGGTGACA CTGTGAATGC AACTCTAATG 
CAGCCTGGCA TAAATGGGCC TATGGGCACT AACTTTCAAG TGAACACAAA TAAAGGAGGA 
GGTGTGTGGG AGTCTGGGGC AACGAATTCC CAGAATGCAT CGTGGGGAAG TGGAAATGGC 
GCAAATTCTG GAGGAAGTCG ACGAGGATGG GGAACTCCTG CACAAAACAC TGGCACTAAT 
ATACCCAGCG TGGAGTGGAG CAAACTGCCT AGCAATCAGC ATTCCAATGA CAGTGCAAAT 
GGCAATGGTA AGAAGTTTAC AAATGGATGG AAATCTACTG AGGAAGAGGA TCAGGCTTCT 
GCCGCATCTC AGACAACTGA GCAGAACAGT GTGTGGGCCA AAACAGGAGG TACAGTGGAG 
AGTGAAGGTA GTACAGAAAG CACTGGACGC CTCGAGGAGA AAGCCACTGG GGAAAATCAG 
AGTAGAGATC GGAGAAAAAT TGATCAGCAC ACATTACTCC AAAGCATCGT AAACAGAACT 
GACTTGGATC CACGTGTCCT CTCCAACTCT GGTTGGGGAC AGACTCCTAT TAAGCAGAAT 
ACTGCCTGGG ATACGGAAAC ATCACCTAGA GGGGAAAGAA AGACTGACAA TGGGACAGAG 
GCCTGGGGAA GCTCTGCAAC ACAGACTTTT AACTCAGGGG CATGTACAGA TAAGACTAGC 
CCTACTAGTA ATGATACCTC ATCTGTATCG GGGTGGGGAG ATCCCAAACC TGCTCTGAGG 
TGGGGAGATT CCAAAGGCTC AAGCTGCCAG GGGGGATGGG AAGATGATGC TGCTGCTACA 
GGAATGGTCA AGAGCAATCA GTGGGGGAAC AGCAAAGAAG AGAAGTCCGC ATGGAATGAT 
TCGCAAAAGA GCAAACAGGG ATGGGGTGAT GGACAGAAAT CAAACCAAGG GTGGTCCGTT 
TCTGCCAGTG ATAACTGGGG AGAAACGTCA AGGAGTAACC ATTGGGGTGA GGCTAATAAG 
AAATCTAGCT CAGGAGGTAG TGACAGTGAC AGGTCTGTTT CTGGTTGGAA TGAACTTGGT 
AAAACTAGTT CTTTTACTTG GGGAAATAAC ATAAATCCAA ATAATTCGTC AGGATGGGAT 
GAATCTTCTA AACCTAATCC TTCCCAGGGA TGGGGAGATC CTCCAAAGTC TAATCAGTCT 
CTAGGTTGGG GAGATTCGTC AAAGCCAGTC ACTTCTCCAG ACTGGAACAA GCAACAAGAC 
ATTGTCGGGT CTTGGGGAAT CCCACCAGCA ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG 
GGAGGACCTA TACCAGCCCC AACAAAAGAA GAAGAGCCCA CAGGCTGGGA GGAACCATCC 
CCAGAATCCA TACGTCGCAA AATGGAGATC GATGACGGAA CTTCAGCTTG GGGAGATCCG 
AGCAAATACA ACTACAAAAA TGTGAACATG TGGAACAAAA ATGTCCCAAA TGGCAGCAGC 
CGTTCAGACC AGCAAGCACA GGTACACCAG TTGCTACCAT CTGCAAGTGC CATCTCAAAC 
AAAGAGGCAG GCAGTGGCTC TGGCTGGGGT GAGCCCTGGG GGGAGCCTTC TACTCCAGCC 
ACAACTGTGG ATAATGGTAC TTCAGCATGG GGTAAACCCA TAGACAGTGG TCCCAGCTGG 
GGGGAGCCCA TTGCTGCAGC ATCCAACACA TCCACGTGGG GCTCCAGCTC TGTTGGTCCT 
CAAGCATTAA GCAAATCTGG GCCAAAATCT ATGCAAGATG GCTGGTGTGG TGATGATATG 
CCATTGCCTG GAAATCGCCC CACTGGCTGG GAAGAGGAAG AGGATGTAGA GATTGGAATG 
TGGAATAGTA ATTCATCTCA AGAGCTTAAC TCATCTTTAA ATTGGCCTCC ATATACAAAG 
AAAATGTCAT CGAAGGGTCT GAGTGGCAAA AAAAGGAGAA GGGAAAGGGG AACCATGAAA 
GGTGGAAACA AACAAGAAGA AGCATGGATA AATCCATTCG TTAAACAGTT TTCAAATATC 
AGTTTTTCGA GAGACTCACC CGAAGAAAAT GTACAGAGCA ATAAGATGGA TCTTTCCGGA 
GGAATGTTAC AGGACAAACG AATGGAGATA GATAAACATA GCCTAAATAT TGGTGATTAC 
AATCGAACGG TCGGGAAAGG CCCTGGTTCT CGGCCTCAGA TTTCCAAAGA GTCTTCCATG 
GAGCGCAGCC CTTACTTTGA TAAGGATGGC ATTGTAGCAG ATGAATCCCA AAACATGCAG 
TTTATGTCCA GTCAAAGCAT GAAGCTTCCC CCTTCAAATA GTGCACTACC TAACCAGGCC 
CTTGGCTCCA TAGCAGGGCT GGGTATGCAA AACTTGAATT CTGTTAGACA GAATGGCAAT 
CCCAGTATGT TTGGTGTTGG AAACACAGCA GCACAACCCC GGGGCATGCA GCAGCCTCCA 
GCACAACCTC TTAGTTCATC TCAGCCTAAT CTTCGTGCTC AAGTGCCTCC TCCATTACTC 
TCCCCTCAGG TTCCAGTTTC ATTGCTGAAG TATGCACCAA ACAACGGTGG CCTGAATCCG 
CTCTTTGGCC CTCAACAGGT AGCCATGCTG AACCAGCTAT CCCAACTAAA CCAGCTTTCT 
CAGATCTCCC AGTTACAGAG ATTGTTAGCG CAGCAGCAAA GGGCGCAGAG TCAGAGAAGC 
GTGCCTTCTG GGAACCGGCA GCAGCAAGAC CAGCAGGGTC GACCTCTTAG CGTGCAGCAG 
CAGATGATGC AGCAGTCCCG GCAACTCGAT CCCAGCCTGT TGGTGAAGCA GCAGACCCCG 
CCATCTCAGC AGCAGCCACT CCATCAGCCA GCCATGAAAT CCTTCCTCGA GAATGTCATG 
CCCCACACTA CACCTGAGCT GCAGAAAGGG CCATCACCAA TAAATGCTTT CAGCAACTTC 
CCGATAGGCT TGAACTCAAA CTTGAATGTA AATATGGATA TGAACAGTAT TAAAGAGCCA 
CAGTCAAGAC TAAGGAAGTG GACTACAGTG GACAGCATTT CTGTGAACAC ATCTTTGGAT 
CAAAACTCCA GCAAACATGG TGCTATTTCA AGTGGTTTCA GGCTGGAAGA GTCTCCATTT 
GTTCCCTATG ACTTTATGAA CAGCAGTACT TCTCCAGCCA GCCCTCCGGG TTCAATAGGA 
GATGGCTGGC CACGTGCCAA ATCGCCTAAC GGCTCTAGCA GTGTTAACTG GCCACCAGAA 
TTTCGCCCTG GTGAACCATG GAAAGGTTAT CCAAACATTG ACCCTGAAAC TGACCCTTAC 
GTCACTCCTG GCAGTGTCAT AAACAATCTT TCAATTAATA CTGTGCGGGA AGTTGACCAC 
CTCAGGGACA GGAACAGTGG GTCATCCTCA TCCTTGAACA CCACGCTGCC TTCAACTAGT 
GCCTGGTCAT CCATTCGTGC CTCCAACTAC AACGTTCCCC TCAGCAGTAC AGCACAAAGC 
ACTTCAGCCA GAAATAGTGA TTCCAAATTG ACATGGTCTC CTGGTTCAGT TACAAACACC 
TCTCTGGCTC ATGAGCTGTG GAAGGTCCCT TTACCACCTA AAAACATCAC TGCTCCGTCC 
CGCCCACCTC CGGGACTCAC TGGTCAGAAG CCACCCTTGT CTACGTGGGA TAACTCTCCC 
CTTCGTGTAG GTGGAGGATG GGGAAATTCT GACGCCAGGT ACACCCCAGG TTCCAGTTGG 
GGTGAGAGCA GCTCAGGGAG AATAACAAAT TGGCTTGTTC TAAAGAACCT CACACCTCAG 
ATTGACGGCT CAACTCTGCG TACTCTGTGC ATGCAGCATG GCCCACTGAT AACATTCCAC 
CTGAACCTCC CACATGGAAA TGCTTTGGTC CGTTACAGTT CAAAAGAAGA GGTAGTGAAG 
GCACAAAAGT CTCTGCACAT GTGTGTATTG GGGAACACTA CTATTCTTGC TGAGTTTGCC 
AGTGAAGAGG AGATCAGCCG TTTCTTTGCA CAAAGTCAGT CTCTGACCCC TTCTCCCGGC 
TGGCAGTCTC TCGGGTCCAG CCAGAGCCGG CTGGGCTCCC TCGACTGTTC CCACTCGTTC 
TCCAGCCGGA CCGATCTCAA TCACTGGAAT GGTGCTGGGC TGTCGGGAAC TAACTGTGGA 
GACCTTCACG GCACTTCACT CTGGGGGACC CCACATTATT CCACAAGCCT GTGGGGTCCT 
CCCAGCAGCA GCGACCCCCG GGGAATTAGC AGCCCATCCC CCATTAATGC TTTTCTTTCT 
GTTGACCACC TGGGTGGGGG TGGAGAGTCC ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_547086.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_547086.3 Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X9, mRNA.

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CloneID	OCe07960
Clone ID Related Accession (Same CDS sequence)	XM_005621403.2 , XM_547086.5 , XM_005621403.3
Accession Version	XM_547086.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	trinucleotide repeat-containing gene 6A protein isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_547086.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA AGAAAAAGAA AAAAGACGAC AAGAAAAAGA AGGAAGCTGC TCAAAAGAAG 
GCCACTGAAC AAAAAATCAA AGTGCCAGAA CAGATAAAGC CCAGTGTAAG CCAGCCTCAG 
CCTGCCAACT CTAATAACGG CACTTCCACA GCAACCAGCA CTAATAATAA TGCCAAGCGA 
GCCACAGCCA ACAATCCGCA GCAGCAGCAG CAGCCACAGC CGCCACAGCA GCCACAGCAG 
CCGCCGCAGC AGCCACCGCA GCCGCCACAG CAGCAGCCGC AGCCAGCACA GGCCTTGCCT 
CGGTATCCTC GGGAAGTACC ACCACGATTT CGCCACCAGG AACACAAACA GCTTCTAAAG 
AGGGGTCAGC ATTTTCCTGT CATAGCAGCA AACCTGGGAT CTGCTGTTAA AGTGTTAAGC 
AGCCAGTCAG AAAGCAGTGC TTTAACAAAT CAACAGCCAC AAAATAACGG AGAGGTGCAG 
AACAGCAAAA ACCAGTCAGA TATAAACCAC AGTACTTCAG GATCCCATTA TGAAAATTCA 
CAGCGGGGAC CTGTGTCTTC TCCGAGTGAC TCTAGCACAA ATTGTAAGAA TGCTGTTGTA 
AACGACTCCC CTGAGACAGA AGCATGGCCC TCAGTCCCTG GCAGTGATCC AGAGTTGGCT 
TCAGAATGTA TGGATGCTGA TTCTGCCTCC AGTTCTGAAT CAGAGAGAAA CATCACTATC 
ATGGCTTCAG GGAGCACAGG TGGTGAAAAA GATGGCCTTC GGAATAGCAC TGGACTTGGT 
TCCCAAAACA AGTTTGTGGT TGGTAGCAGC AGCAATAATG TGGGCCATGG AAGTAGTCCT 
GGGCCATGGG GTTTTTCCCA TGGAGCCATA ATAAGCACAT GTCAGGTCTC TGTGGATGCT 
CCTGAAAGCA AATCAGAAAG TAGCAACAAT AGAATGAATG CTTGGGGCAC TGTAAGTTCT 
TCATCAAATG GAGGGTTAAA TCCAAGCACT TTGAATTCAG CTAGCAACCA TGGTGCCTGG 
CCAGTATTAG ACAACAACGG ACTTGCCCTC AAAGGGCCTG TAGGGAGCGG TAGTTCTGGC 
ATCAATATTC AGTGTAGTAC CATAGGCCAG ATGCCTAACA ATCAGAGTAT TAACTCTAAA 
GTGGGTGGTT CTTCCACCCA TGGTACCTGG GGAAGCCTTC AGGAAACTTG TGAATCTGAA 
GTAAGTGGTA CACAGAAGGT TTCATTCAGT GGTCAACCTC AGAATATTAC CACTGAGATG 
ACTGGACCAA ATAACACTAC TAACTTTATG ACCTCTAGTT TACCAAACTC CGGTTCAGTA 
CAGAATAACG AACTGCCTAG TAATACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT 
CAGATACAGG CTCCATCGGT TATGAATGGC ACTTCCCTTT CTCATCTTAG CAATGGAGAG 
CCAAAAAGTG GAGGCTCTTA CGGTACTACG TGGGGTGCCT ATGGTTCTAA TTACTCTGGA 
GACAAATGTT CGAGCCCTAA CGGCCAAGCT AATGGTGACA CTGTGAATGC AACTCTAATG 
CAGCCTGGCA TAAATGGGCC TATGGGCACT AACTTTCAAG TGAACACAAA TAAAGGAGGA 
GGTGTGTGGG AGTCTGGGGC AACGAATTCC CAGAATGCAT CGTGGGGAAG TGGAAATGGC 
GCAAATTCTG GAGGAAGTCG ACGAGGATGG GGAACTCCTG CACAAAACAC TGGCACTAAT 
ATACCCAGCG TGGAGTGGAG CAAACTGCCT AGCAATCAGC ATTCCAATGA CAGTGCAAAT 
GGCAATGGTA AGAAGTTTAC AAATGGATGG AAATCTACTG AGGAAGAGGA TCAGGCTTCT 
GCCGCATCTC AGACAACTGA GCAGAACAGT GTGTGGGCCA AAACAGGAGG TACAGTGGAG 
AGTGAAGGTA GTACAGAAAG CACTGGACGC CTCGAGGAGA AAGCCACTGG GGAAAATCAG 
AGTAGAGATC GGAGAAAAAT TGATCAGCAC ACATTACTCC AAAGCATCGT AAACAGAACT 
GACTTGGATC CACGTGTCCT CTCCAACTCT GGTTGGGGAC AGACTCCTAT TAAGCAGAAT 
ACTGCCTGGG ATACGGAAAC ATCACCTAGA GGGGAAAGAA AGACTGACAA TGGGACAGAG 
GCCTGGGGAA GCTCTGCAAC ACAGACTTTT AACTCAGGGG CATGTACAGA TAAGACTAGC 
CCTACTAGTA ATGATACCTC ATCTGTATCG GGGTGGGGAG ATCCCAAACC TGCTCTGAGG 
TGGGGAGATT CCAAAGGCTC AAGCTGCCAG GGGGGATGGG AAGATGATGC TGCTGCTACA 
GGAATGGTCA AGAGCAATCA GTGGGGGAAC AGCAAAGAAG AGAAGTCCGC ATGGAATGAT 
TCGCAAAAGA GCAAACAGGG ATGGGGTGAT GGACAGAAAT CAAACCAAGG GTGGTCCGTT 
TCTGCCAGTG ATAACTGGGG AGAAACGTCA AGGAGTAACC ATTGGGGTGA GGCTAATAAG 
AAATCTAGCT CAGGAGGTAG TGACAGTGAC AGGTCTGTTT CTGGTTGGAA TGAACTTGGT 
AAAACTAGTT CTTTTACTTG GGGAAATAAC ATAAATCCAA ATAATTCGTC AGGATGGGAT 
GAATCTTCTA AACCTAATCC TTCCCAGGGA TGGGGAGATC CTCCAAAGTC TAATCAGTCT 
CTAGGTTGGG GAGATTCGTC AAAGCCAGTC ACTTCTCCAG ACTGGAACAA GCAACAAGAC 
ATTGTCGGGT CTTGGGGAAT CCCACCAGCA ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG 
GGAGGACCTA TACCAGCCCC AACAAAAGAA GAAGAGCCCA CAGGCTGGGA GGAACCATCC 
CCAGAATCCA TACGTCGCAA AATGGAGATC GATGACGGAA CTTCAGCTTG GGGAGATCCG 
AGCAAATACA ACTACAAAAA TGTGAACATG TGGAACAAAA ATGTCCCAAA TGGCAGCAGC 
CGTTCAGACC AGCAAGCACA GGTACACCAG TTGCTACCAT CTGCAAGTGC CATCTCAAAC 
AAAGAGGCAG GCAGTGGCTC TGGCTGGGGT GAGCCCTGGG GGGAGCCTTC TACTCCAGCC 
ACAACTGTGG ATAATGGTAC TTCAGCATGG GGTAAACCCA TAGACAGTGG TCCCAGCTGG 
GGGGAGCCCA TTGCTGCAGC ATCCAACACA TCCACGTGGG GCTCCAGCTC TGTTGGTCCT 
CAAGCATTAA GCAAATCTGG GCCAAAATCT ATGCAAGATG GCTGGTGTGG TGATGATATG 
CCATTGCCTG GAAATCGCCC CACTGGCTGG GAAGAGGAAG AGGATGTAGA GATTGGAATG 
TGGAATAGTA ATTCATCTCA AGAGCTTAAC TCATCTTTAA ATTGGCCTCC ATATACAAAG 
AAAATGTCAT CGAAGGGTCT GAGTGGCAAA AAAAGGAGAA GGGAAAGGGG AACCATGAAA 
GGTGGAAACA AACAAGAAGA AGCATGGATA AATCCATTCG TTAAACAGTT TTCAAATATC 
AGTTTTTCGA GAGACTCACC CGAAGAAAAT GTACAGAGCA ATAAGATGGA TCTTTCCGGA 
GGAATGTTAC AGGACAAACG AATGGAGATA GATAAACATA GCCTAAATAT TGGTGATTAC 
AATCGAACGG TCGGGAAAGG CCCTGGTTCT CGGCCTCAGA TTTCCAAAGA GTCTTCCATG 
GAGCGCAGCC CTTACTTTGA TAAGGATGGC ATTGTAGCAG ATGAATCCCA AAACATGCAG 
TTTATGTCCA GTCAAAGCAT GAAGCTTCCC CCTTCAAATA GTGCACTACC TAACCAGGCC 
CTTGGCTCCA TAGCAGGGCT GGGTATGCAA AACTTGAATT CTGTTAGACA GAATGGCAAT 
CCCAGTATGT TTGGTGTTGG AAACACAGCA GCACAACCCC GGGGCATGCA GCAGCCTCCA 
GCACAACCTC TTAGTTCATC TCAGCCTAAT CTTCGTGCTC AAGTGCCTCC TCCATTACTC 
TCCCCTCAGG TTCCAGTTTC ATTGCTGAAG TATGCACCAA ACAACGGTGG CCTGAATCCG 
CTCTTTGGCC CTCAACAGGT AGCCATGCTG AACCAGCTAT CCCAACTAAA CCAGCTTTCT 
CAGATCTCCC AGTTACAGAG ATTGTTAGCG CAGCAGCAAA GGGCGCAGAG TCAGAGAAGC 
GTGCCTTCTG GGAACCGGCA GCAGCAAGAC CAGCAGGGTC GACCTCTTAG CGTGCAGCAG 
CAGATGATGC AGCAGTCCCG GCAACTCGAT CCCAGCCTGT TGGTGAAGCA GCAGACCCCG 
CCATCTCAGC AGCAGCCACT CCATCAGCCA GCCATGAAAT CCTTCCTCGA GAATGTCATG 
CCCCACACTA CACCTGAGCT GCAGAAAGGG CCATCACCAA TAAATGCTTT CAGCAACTTC 
CCGATAGGCT TGAACTCAAA CTTGAATGTA AATATGGATA TGAACAGTAT TAAAGAGCCA 
CAGTCAAGAC TAAGGAAGTG GACTACAGTG GACAGCATTT CTGTGAACAC ATCTTTGGAT 
CAAAACTCCA GCAAACATGG TGCTATTTCA AGTGGTTTCA GGCTGGAAGA GTCTCCATTT 
GTTCCCTATG ACTTTATGAA CAGCAGTACT TCTCCAGCCA GCCCTCCGGG TTCAATAGGA 
GATGGCTGGC CACGTGCCAA ATCGCCTAAC GGCTCTAGCA GTGTTAACTG GCCACCAGAA 
TTTCGCCCTG GTGAACCATG GAAAGGTTAT CCAAACATTG ACCCTGAAAC TGACCCTTAC 
GTCACTCCTG GCAGTGTCAT AAACAATCTT TCAATTAATA CTGTGCGGGA AGTTGACCAC 
CTCAGGGACA GGAACAGTGG GTCATCCTCA TCCTTGAACA CCACGCTGCC TTCAACTAGT 
GCCTGGTCAT CCATTCGTGC CTCCAACTAC AACGTTCCCC TCAGCAGTAC AGCACAAAGC 
ACTTCAGCCA GAAATAGTGA TTCCAAATTG ACATGGTCTC CTGGTTCAGT TACAAACACC 
TCTCTGGCTC ATGAGCTGTG GAAGGTCCCT TTACCACCTA AAAACATCAC TGCTCCGTCC 
CGCCCACCTC CGGGACTCAC TGGTCAGAAG CCACCCTTGT CTACGTGGGA TAACTCTCCC 
CTTCGTGTAG GTGGAGGATG GGGAAATTCT GACGCCAGGT ACACCCCAGG TTCCAGTTGG 
GGTGAGAGCA GCTCAGGGAG AATAACAAAT TGGCTTGTTC TAAAGAACCT CACACCTCAG 
ATTGACGGCT CAACTCTGCG TACTCTGTGC ATGCAGCATG GCCCACTGAT AACATTCCAC 
CTGAACCTCC CACATGGAAA TGCTTTGGTC CGTTACAGTT CAAAAGAAGA GGTAGTGAAG 
GCACAAAAGT CTCTGCACAT GTGTGTATTG GGGAACACTA CTATTCTTGC TGAGTTTGCC 
AGTGAAGAGG AGATCAGCCG TTTCTTTGCA CAAAGTCAGT CTCTGACCCC TTCTCCCGGC 
TGGCAGTCTC TCGGGTCCAG CCAGAGCCGG CTGGGCTCCC TCGACTGTTC CCACTCGTTC 
TCCAGCCGGA CCGATCTCAA TCACTGGAAT GGTGCTGGGC TGTCGGGAAC TAACTGTGGA 
GACCTTCACG GCACTTCACT CTGGGGGACC CCACATTATT CCACAAGCCT GTGGGGTCCT 
CCCAGCAGCA GCGACCCCCG GGGAATTAGC AGCCCATCCC CCATTAATGC TTTTCTTTCT 
GTTGACCACC TGGGTGGGGG TGGAGAGTCC ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_547086.3
CDS	177..5972
Translation	MEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNPQQQQQPQPPQQPQQPPQQPPQPPQQQPQPAQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLSSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSPSDSSTNCKNAVVNDSPETEAWPSVPGSDPELASECMDADSASSSESERNITIMASGSTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSPGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLDNNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVGGSSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVMNGTSLSHLSNGEPKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGINGPMGTNFQVNTNKGGGVWESGATNSQNASWGSGNGANSGGSRRGWGTPAQNTGTNIPSVEWSKLPSNQHSNDSANGNGKKFTNGWKSTEEEDQASAASQTTEQNSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACTDKTSPTSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDAAATGMVKSNQWGNSKEEKSAWNDSQKSKQGWGDGQKSNQGWSVSASDNWGETSRSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVTSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPTKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSASAISNKEAGSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASNTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGTMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERSPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPSLLVKQQTPPSQQQPLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_547086.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_547086.5

ORF Insert Sequence:

ATGGAAGAAA AGAAAAAGAA AAAAGACGAC AAGAAAAAGA AGGAAGCTGC TCAAAAGAAG 
GCCACTGAAC AAAAAATCAA AGTGCCAGAA CAGATAAAGC CCAGTGTAAG CCAGCCTCAG 
CCTGCCAACT CTAATAACGG CACTTCCACA GCAACCAGCA CTAATAATAA TGCCAAGCGA 
GCCACAGCCA ACAATCCGCA GCAGCAGCAG CAGCCACAGC CGCCACAGCA GCCACAGCAG 
CCGCCGCAGC AGCCACCGCA GCCGCCACAG CAGCAGCCGC AGCCAGCACA GGCCTTGCCT 
CGGTATCCTC GGGAAGTACC ACCACGATTT CGCCACCAGG AACACAAACA GCTTCTAAAG 
AGGGGTCAGC ATTTTCCTGT CATAGCAGCA AACCTGGGAT CTGCTGTTAA AGTGTTAAGC 
AGCCAGTCAG AAAGCAGTGC TTTAACAAAT CAACAGCCAC AAAATAACGG AGAGGTGCAG 
AACAGCAAAA ACCAGTCAGA TATAAACCAC AGTACTTCAG GATCCCATTA TGAAAATTCA 
CAGCGGGGAC CTGTGTCTTC TCCGAGTGAC TCTAGCACAA ATTGTAAGAA TGCTGTTGTA 
AACGACTCCC CTGAGACAGA AGCATGGCCC TCAGTCCCTG GCAGTGATCC AGAGTTGGCT 
TCAGAATGTA TGGATGCTGA TTCTGCCTCC AGTTCTGAAT CAGAGAGAAA CATCACTATC 
ATGGCTTCAG GGAGCACAGG TGGTGAAAAA GATGGCCTTC GGAATAGCAC TGGACTTGGT 
TCCCAAAACA AGTTTGTGGT TGGTAGCAGC AGCAATAATG TGGGCCATGG AAGTAGTCCT 
GGGCCATGGG GTTTTTCCCA TGGAGCCATA ATAAGCACAT GTCAGGTCTC TGTGGATGCT 
CCTGAAAGCA AATCAGAAAG TAGCAACAAT AGAATGAATG CTTGGGGCAC TGTAAGTTCT 
TCATCAAATG GAGGGTTAAA TCCAAGCACT TTGAATTCAG CTAGCAACCA TGGTGCCTGG 
CCAGTATTAG ACAACAACGG ACTTGCCCTC AAAGGGCCTG TAGGGAGCGG TAGTTCTGGC 
ATCAATATTC AGTGTAGTAC CATAGGCCAG ATGCCTAACA ATCAGAGTAT TAACTCTAAA 
GTGGGTGGTT CTTCCACCCA TGGTACCTGG GGAAGCCTTC AGGAAACTTG TGAATCTGAA 
GTAAGTGGTA CACAGAAGGT TTCATTCAGT GGTCAACCTC AGAATATTAC CACTGAGATG 
ACTGGACCAA ATAACACTAC TAACTTTATG ACCTCTAGTT TACCAAACTC CGGTTCAGTA 
CAGAATAACG AACTGCCTAG TAATACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT 
CAGATACAGG CTCCATCGGT TATGAATGGC ACTTCCCTTT CTCATCTTAG CAATGGAGAG 
CCAAAAAGTG GAGGCTCTTA CGGTACTACG TGGGGTGCCT ATGGTTCTAA TTACTCTGGA 
GACAAATGTT CGAGCCCTAA CGGCCAAGCT AATGGTGACA CTGTGAATGC AACTCTAATG 
CAGCCTGGCA TAAATGGGCC TATGGGCACT AACTTTCAAG TGAACACAAA TAAAGGAGGA 
GGTGTGTGGG AGTCTGGGGC AACGAATTCC CAGAATGCAT CGTGGGGAAG TGGAAATGGC 
GCAAATTCTG GAGGAAGTCG ACGAGGATGG GGAACTCCTG CACAAAACAC TGGCACTAAT 
ATACCCAGCG TGGAGTGGAG CAAACTGCCT AGCAATCAGC ATTCCAATGA CAGTGCAAAT 
GGCAATGGTA AGAAGTTTAC AAATGGATGG AAATCTACTG AGGAAGAGGA TCAGGCTTCT 
GCCGCATCTC AGACAACTGA GCAGAACAGT GTGTGGGCCA AAACAGGAGG TACAGTGGAG 
AGTGAAGGTA GTACAGAAAG CACTGGACGC CTCGAGGAGA AAGCCACTGG GGAAAATCAG 
AGTAGAGATC GGAGAAAAAT TGATCAGCAC ACATTACTCC AAAGCATCGT AAACAGAACT 
GACTTGGATC CACGTGTCCT CTCCAACTCT GGTTGGGGAC AGACTCCTAT TAAGCAGAAT 
ACTGCCTGGG ATACGGAAAC ATCACCTAGA GGGGAAAGAA AGACTGACAA TGGGACAGAG 
GCCTGGGGAA GCTCTGCAAC ACAGACTTTT AACTCAGGGG CATGTACAGA TAAGACTAGC 
CCTACTAGTA ATGATACCTC ATCTGTATCG GGGTGGGGAG ATCCCAAACC TGCTCTGAGG 
TGGGGAGATT CCAAAGGCTC AAGCTGCCAG GGGGGATGGG AAGATGATGC TGCTGCTACA 
GGAATGGTCA AGAGCAATCA GTGGGGGAAC AGCAAAGAAG AGAAGTCCGC ATGGAATGAT 
TCGCAAAAGA GCAAACAGGG ATGGGGTGAT GGACAGAAAT CAAACCAAGG GTGGTCCGTT 
TCTGCCAGTG ATAACTGGGG AGAAACGTCA AGGAGTAACC ATTGGGGTGA GGCTAATAAG 
AAATCTAGCT CAGGAGGTAG TGACAGTGAC AGGTCTGTTT CTGGTTGGAA TGAACTTGGT 
AAAACTAGTT CTTTTACTTG GGGAAATAAC ATAAATCCAA ATAATTCGTC AGGATGGGAT 
GAATCTTCTA AACCTAATCC TTCCCAGGGA TGGGGAGATC CTCCAAAGTC TAATCAGTCT 
CTAGGTTGGG GAGATTCGTC AAAGCCAGTC ACTTCTCCAG ACTGGAACAA GCAACAAGAC 
ATTGTCGGGT CTTGGGGAAT CCCACCAGCA ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG 
GGAGGACCTA TACCAGCCCC AACAAAAGAA GAAGAGCCCA CAGGCTGGGA GGAACCATCC 
CCAGAATCCA TACGTCGCAA AATGGAGATC GATGACGGAA CTTCAGCTTG GGGAGATCCG 
AGCAAATACA ACTACAAAAA TGTGAACATG TGGAACAAAA ATGTCCCAAA TGGCAGCAGC 
CGTTCAGACC AGCAAGCACA GGTACACCAG TTGCTACCAT CTGCAAGTGC CATCTCAAAC 
AAAGAGGCAG GCAGTGGCTC TGGCTGGGGT GAGCCCTGGG GGGAGCCTTC TACTCCAGCC 
ACAACTGTGG ATAATGGTAC TTCAGCATGG GGTAAACCCA TAGACAGTGG TCCCAGCTGG 
GGGGAGCCCA TTGCTGCAGC ATCCAACACA TCCACGTGGG GCTCCAGCTC TGTTGGTCCT 
CAAGCATTAA GCAAATCTGG GCCAAAATCT ATGCAAGATG GCTGGTGTGG TGATGATATG 
CCATTGCCTG GAAATCGCCC CACTGGCTGG GAAGAGGAAG AGGATGTAGA GATTGGAATG 
TGGAATAGTA ATTCATCTCA AGAGCTTAAC TCATCTTTAA ATTGGCCTCC ATATACAAAG 
AAAATGTCAT CGAAGGGTCT GAGTGGCAAA AAAAGGAGAA GGGAAAGGGG AACCATGAAA 
GGTGGAAACA AACAAGAAGA AGCATGGATA AATCCATTCG TTAAACAGTT TTCAAATATC 
AGTTTTTCGA GAGACTCACC CGAAGAAAAT GTACAGAGCA ATAAGATGGA TCTTTCCGGA 
GGAATGTTAC AGGACAAACG AATGGAGATA GATAAACATA GCCTAAATAT TGGTGATTAC 
AATCGAACGG TCGGGAAAGG CCCTGGTTCT CGGCCTCAGA TTTCCAAAGA GTCTTCCATG 
GAGCGCAGCC CTTACTTTGA TAAGGATGGC ATTGTAGCAG ATGAATCCCA AAACATGCAG 
TTTATGTCCA GTCAAAGCAT GAAGCTTCCC CCTTCAAATA GTGCACTACC TAACCAGGCC 
CTTGGCTCCA TAGCAGGGCT GGGTATGCAA AACTTGAATT CTGTTAGACA GAATGGCAAT 
CCCAGTATGT TTGGTGTTGG AAACACAGCA GCACAACCCC GGGGCATGCA GCAGCCTCCA 
GCACAACCTC TTAGTTCATC TCAGCCTAAT CTTCGTGCTC AAGTGCCTCC TCCATTACTC 
TCCCCTCAGG TTCCAGTTTC ATTGCTGAAG TATGCACCAA ACAACGGTGG CCTGAATCCG 
CTCTTTGGCC CTCAACAGGT AGCCATGCTG AACCAGCTAT CCCAACTAAA CCAGCTTTCT 
CAGATCTCCC AGTTACAGAG ATTGTTAGCG CAGCAGCAAA GGGCGCAGAG TCAGAGAAGC 
GTGCCTTCTG GGAACCGGCA GCAGCAAGAC CAGCAGGGTC GACCTCTTAG CGTGCAGCAG 
CAGATGATGC AGCAGTCCCG GCAACTCGAT CCCAGCCTGT TGGTGAAGCA GCAGACCCCG 
CCATCTCAGC AGCAGCCACT CCATCAGCCA GCCATGAAAT CCTTCCTCGA GAATGTCATG 
CCCCACACTA CACCTGAGCT GCAGAAAGGG CCATCACCAA TAAATGCTTT CAGCAACTTC 
CCGATAGGCT TGAACTCAAA CTTGAATGTA AATATGGATA TGAACAGTAT TAAAGAGCCA 
CAGTCAAGAC TAAGGAAGTG GACTACAGTG GACAGCATTT CTGTGAACAC ATCTTTGGAT 
CAAAACTCCA GCAAACATGG TGCTATTTCA AGTGGTTTCA GGCTGGAAGA GTCTCCATTT 
GTTCCCTATG ACTTTATGAA CAGCAGTACT TCTCCAGCCA GCCCTCCGGG TTCAATAGGA 
GATGGCTGGC CACGTGCCAA ATCGCCTAAC GGCTCTAGCA GTGTTAACTG GCCACCAGAA 
TTTCGCCCTG GTGAACCATG GAAAGGTTAT CCAAACATTG ACCCTGAAAC TGACCCTTAC 
GTCACTCCTG GCAGTGTCAT AAACAATCTT TCAATTAATA CTGTGCGGGA AGTTGACCAC 
CTCAGGGACA GGAACAGTGG GTCATCCTCA TCCTTGAACA CCACGCTGCC TTCAACTAGT 
GCCTGGTCAT CCATTCGTGC CTCCAACTAC AACGTTCCCC TCAGCAGTAC AGCACAAAGC 
ACTTCAGCCA GAAATAGTGA TTCCAAATTG ACATGGTCTC CTGGTTCAGT TACAAACACC 
TCTCTGGCTC ATGAGCTGTG GAAGGTCCCT TTACCACCTA AAAACATCAC TGCTCCGTCC 
CGCCCACCTC CGGGACTCAC TGGTCAGAAG CCACCCTTGT CTACGTGGGA TAACTCTCCC 
CTTCGTGTAG GTGGAGGATG GGGAAATTCT GACGCCAGGT ACACCCCAGG TTCCAGTTGG 
GGTGAGAGCA GCTCAGGGAG AATAACAAAT TGGCTTGTTC TAAAGAACCT CACACCTCAG 
ATTGACGGCT CAACTCTGCG TACTCTGTGC ATGCAGCATG GCCCACTGAT AACATTCCAC 
CTGAACCTCC CACATGGAAA TGCTTTGGTC CGTTACAGTT CAAAAGAAGA GGTAGTGAAG 
GCACAAAAGT CTCTGCACAT GTGTGTATTG GGGAACACTA CTATTCTTGC TGAGTTTGCC 
AGTGAAGAGG AGATCAGCCG TTTCTTTGCA CAAAGTCAGT CTCTGACCCC TTCTCCCGGC 
TGGCAGTCTC TCGGGTCCAG CCAGAGCCGG CTGGGCTCCC TCGACTGTTC CCACTCGTTC 
TCCAGCCGGA CCGATCTCAA TCACTGGAAT GGTGCTGGGC TGTCGGGAAC TAACTGTGGA 
GACCTTCACG GCACTTCACT CTGGGGGACC CCACATTATT CCACAAGCCT GTGGGGTCCT 
CCCAGCAGCA GCGACCCCCG GGGAATTAGC AGCCCATCCC CCATTAATGC TTTTCTTTCT 
GTTGACCACC TGGGTGGGGG TGGAGAGTCC ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_005621403.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005621460.1 Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X6, mRNA.

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CloneID	OCe07960
Clone ID Related Accession (Same CDS sequence)	XM_005621403.2 , XM_547086.5 , XM_005621403.3
Accession Version	XM_005621403.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	trinucleotide repeat-containing gene 6A protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005621403.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA AGAAAAAGAA AAAAGACGAC AAGAAAAAGA AGGAAGCTGC TCAAAAGAAG 
GCCACTGAAC AAAAAATCAA AGTGCCAGAA CAGATAAAGC CCAGTGTAAG CCAGCCTCAG 
CCTGCCAACT CTAATAACGG CACTTCCACA GCAACCAGCA CTAATAATAA TGCCAAGCGA 
GCCACAGCCA ACAATCCGCA GCAGCAGCAG CAGCCACAGC CGCCACAGCA GCCACAGCAG 
CCGCCGCAGC AGCCACCGCA GCCGCCACAG CAGCAGCCGC AGCCAGCACA GGCCTTGCCT 
CGGTATCCTC GGGAAGTACC ACCACGATTT CGCCACCAGG AACACAAACA GCTTCTAAAG 
AGGGGTCAGC ATTTTCCTGT CATAGCAGCA AACCTGGGAT CTGCTGTTAA AGTGTTAAGC 
AGCCAGTCAG AAAGCAGTGC TTTAACAAAT CAACAGCCAC AAAATAACGG AGAGGTGCAG 
AACAGCAAAA ACCAGTCAGA TATAAACCAC AGTACTTCAG GATCCCATTA TGAAAATTCA 
CAGCGGGGAC CTGTGTCTTC TCCGAGTGAC TCTAGCACAA ATTGTAAGAA TGCTGTTGTA 
AACGACTCCC CTGAGACAGA AGCATGGCCC TCAGTCCCTG GCAGTGATCC AGAGTTGGCT 
TCAGAATGTA TGGATGCTGA TTCTGCCTCC AGTTCTGAAT CAGAGAGAAA CATCACTATC 
ATGGCTTCAG GGAGCACAGG TGGTGAAAAA GATGGCCTTC GGAATAGCAC TGGACTTGGT 
TCCCAAAACA AGTTTGTGGT TGGTAGCAGC AGCAATAATG TGGGCCATGG AAGTAGTCCT 
GGGCCATGGG GTTTTTCCCA TGGAGCCATA ATAAGCACAT GTCAGGTCTC TGTGGATGCT 
CCTGAAAGCA AATCAGAAAG TAGCAACAAT AGAATGAATG CTTGGGGCAC TGTAAGTTCT 
TCATCAAATG GAGGGTTAAA TCCAAGCACT TTGAATTCAG CTAGCAACCA TGGTGCCTGG 
CCAGTATTAG ACAACAACGG ACTTGCCCTC AAAGGGCCTG TAGGGAGCGG TAGTTCTGGC 
ATCAATATTC AGTGTAGTAC CATAGGCCAG ATGCCTAACA ATCAGAGTAT TAACTCTAAA 
GTGGGTGGTT CTTCCACCCA TGGTACCTGG GGAAGCCTTC AGGAAACTTG TGAATCTGAA 
GTAAGTGGTA CACAGAAGGT TTCATTCAGT GGTCAACCTC AGAATATTAC CACTGAGATG 
ACTGGACCAA ATAACACTAC TAACTTTATG ACCTCTAGTT TACCAAACTC CGGTTCAGTA 
CAGAATAACG AACTGCCTAG TAATACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT 
CAGATACAGG CTCCATCGGT TATGAATGGC ACTTCCCTTT CTCATCTTAG CAATGGAGAG 
CCAAAAAGTG GAGGCTCTTA CGGTACTACG TGGGGTGCCT ATGGTTCTAA TTACTCTGGA 
GACAAATGTT CGAGCCCTAA CGGCCAAGCT AATGGTGACA CTGTGAATGC AACTCTAATG 
CAGCCTGGCA TAAATGGGCC TATGGGCACT AACTTTCAAG TGAACACAAA TAAAGGAGGA 
GGTGTGTGGG AGTCTGGGGC AACGAATTCC CAGAATGCAT CGTGGGGAAG TGGAAATGGC 
GCAAATTCTG GAGGAAGTCG ACGAGGATGG GGAACTCCTG CACAAAACAC TGGCACTAAT 
ATACCCAGCG TGGAGTGGAG CAAACTGCCT AGCAATCAGC ATTCCAATGA CAGTGCAAAT 
GGCAATGGTA AGAAGTTTAC AAATGGATGG AAATCTACTG AGGAAGAGGA TCAGGCTTCT 
GCCGCATCTC AGACAACTGA GCAGAACAGT GTGTGGGCCA AAACAGGAGG TACAGTGGAG 
AGTGAAGGTA GTACAGAAAG CACTGGACGC CTCGAGGAGA AAGCCACTGG GGAAAATCAG 
AGTAGAGATC GGAGAAAAAT TGATCAGCAC ACATTACTCC AAAGCATCGT AAACAGAACT 
GACTTGGATC CACGTGTCCT CTCCAACTCT GGTTGGGGAC AGACTCCTAT TAAGCAGAAT 
ACTGCCTGGG ATACGGAAAC ATCACCTAGA GGGGAAAGAA AGACTGACAA TGGGACAGAG 
GCCTGGGGAA GCTCTGCAAC ACAGACTTTT AACTCAGGGG CATGTACAGA TAAGACTAGC 
CCTACTAGTA ATGATACCTC ATCTGTATCG GGGTGGGGAG ATCCCAAACC TGCTCTGAGG 
TGGGGAGATT CCAAAGGCTC AAGCTGCCAG GGGGGATGGG AAGATGATGC TGCTGCTACA 
GGAATGGTCA AGAGCAATCA GTGGGGGAAC AGCAAAGAAG AGAAGTCCGC ATGGAATGAT 
TCGCAAAAGA GCAAACAGGG ATGGGGTGAT GGACAGAAAT CAAACCAAGG GTGGTCCGTT 
TCTGCCAGTG ATAACTGGGG AGAAACGTCA AGGAGTAACC ATTGGGGTGA GGCTAATAAG 
AAATCTAGCT CAGGAGGTAG TGACAGTGAC AGGTCTGTTT CTGGTTGGAA TGAACTTGGT 
AAAACTAGTT CTTTTACTTG GGGAAATAAC ATAAATCCAA ATAATTCGTC AGGATGGGAT 
GAATCTTCTA AACCTAATCC TTCCCAGGGA TGGGGAGATC CTCCAAAGTC TAATCAGTCT 
CTAGGTTGGG GAGATTCGTC AAAGCCAGTC ACTTCTCCAG ACTGGAACAA GCAACAAGAC 
ATTGTCGGGT CTTGGGGAAT CCCACCAGCA ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG 
GGAGGACCTA TACCAGCCCC AACAAAAGAA GAAGAGCCCA CAGGCTGGGA GGAACCATCC 
CCAGAATCCA TACGTCGCAA AATGGAGATC GATGACGGAA CTTCAGCTTG GGGAGATCCG 
AGCAAATACA ACTACAAAAA TGTGAACATG TGGAACAAAA ATGTCCCAAA TGGCAGCAGC 
CGTTCAGACC AGCAAGCACA GGTACACCAG TTGCTACCAT CTGCAAGTGC CATCTCAAAC 
AAAGAGGCAG GCAGTGGCTC TGGCTGGGGT GAGCCCTGGG GGGAGCCTTC TACTCCAGCC 
ACAACTGTGG ATAATGGTAC TTCAGCATGG GGTAAACCCA TAGACAGTGG TCCCAGCTGG 
GGGGAGCCCA TTGCTGCAGC ATCCAACACA TCCACGTGGG GCTCCAGCTC TGTTGGTCCT 
CAAGCATTAA GCAAATCTGG GCCAAAATCT ATGCAAGATG GCTGGTGTGG TGATGATATG 
CCATTGCCTG GAAATCGCCC CACTGGCTGG GAAGAGGAAG AGGATGTAGA GATTGGAATG 
TGGAATAGTA ATTCATCTCA AGAGCTTAAC TCATCTTTAA ATTGGCCTCC ATATACAAAG 
AAAATGTCAT CGAAGGGTCT GAGTGGCAAA AAAAGGAGAA GGGAAAGGGG AACCATGAAA 
GGTGGAAACA AACAAGAAGA AGCATGGATA AATCCATTCG TTAAACAGTT TTCAAATATC 
AGTTTTTCGA GAGACTCACC CGAAGAAAAT GTACAGAGCA ATAAGATGGA TCTTTCCGGA 
GGAATGTTAC AGGACAAACG AATGGAGATA GATAAACATA GCCTAAATAT TGGTGATTAC 
AATCGAACGG TCGGGAAAGG CCCTGGTTCT CGGCCTCAGA TTTCCAAAGA GTCTTCCATG 
GAGCGCAGCC CTTACTTTGA TAAGGATGGC ATTGTAGCAG ATGAATCCCA AAACATGCAG 
TTTATGTCCA GTCAAAGCAT GAAGCTTCCC CCTTCAAATA GTGCACTACC TAACCAGGCC 
CTTGGCTCCA TAGCAGGGCT GGGTATGCAA AACTTGAATT CTGTTAGACA GAATGGCAAT 
CCCAGTATGT TTGGTGTTGG AAACACAGCA GCACAACCCC GGGGCATGCA GCAGCCTCCA 
GCACAACCTC TTAGTTCATC TCAGCCTAAT CTTCGTGCTC AAGTGCCTCC TCCATTACTC 
TCCCCTCAGG TTCCAGTTTC ATTGCTGAAG TATGCACCAA ACAACGGTGG CCTGAATCCG 
CTCTTTGGCC CTCAACAGGT AGCCATGCTG AACCAGCTAT CCCAACTAAA CCAGCTTTCT 
CAGATCTCCC AGTTACAGAG ATTGTTAGCG CAGCAGCAAA GGGCGCAGAG TCAGAGAAGC 
GTGCCTTCTG GGAACCGGCA GCAGCAAGAC CAGCAGGGTC GACCTCTTAG CGTGCAGCAG 
CAGATGATGC AGCAGTCCCG GCAACTCGAT CCCAGCCTGT TGGTGAAGCA GCAGACCCCG 
CCATCTCAGC AGCAGCCACT CCATCAGCCA GCCATGAAAT CCTTCCTCGA GAATGTCATG 
CCCCACACTA CACCTGAGCT GCAGAAAGGG CCATCACCAA TAAATGCTTT CAGCAACTTC 
CCGATAGGCT TGAACTCAAA CTTGAATGTA AATATGGATA TGAACAGTAT TAAAGAGCCA 
CAGTCAAGAC TAAGGAAGTG GACTACAGTG GACAGCATTT CTGTGAACAC ATCTTTGGAT 
CAAAACTCCA GCAAACATGG TGCTATTTCA AGTGGTTTCA GGCTGGAAGA GTCTCCATTT 
GTTCCCTATG ACTTTATGAA CAGCAGTACT TCTCCAGCCA GCCCTCCGGG TTCAATAGGA 
GATGGCTGGC CACGTGCCAA ATCGCCTAAC GGCTCTAGCA GTGTTAACTG GCCACCAGAA 
TTTCGCCCTG GTGAACCATG GAAAGGTTAT CCAAACATTG ACCCTGAAAC TGACCCTTAC 
GTCACTCCTG GCAGTGTCAT AAACAATCTT TCAATTAATA CTGTGCGGGA AGTTGACCAC 
CTCAGGGACA GGAACAGTGG GTCATCCTCA TCCTTGAACA CCACGCTGCC TTCAACTAGT 
GCCTGGTCAT CCATTCGTGC CTCCAACTAC AACGTTCCCC TCAGCAGTAC AGCACAAAGC 
ACTTCAGCCA GAAATAGTGA TTCCAAATTG ACATGGTCTC CTGGTTCAGT TACAAACACC 
TCTCTGGCTC ATGAGCTGTG GAAGGTCCCT TTACCACCTA AAAACATCAC TGCTCCGTCC 
CGCCCACCTC CGGGACTCAC TGGTCAGAAG CCACCCTTGT CTACGTGGGA TAACTCTCCC 
CTTCGTGTAG GTGGAGGATG GGGAAATTCT GACGCCAGGT ACACCCCAGG TTCCAGTTGG 
GGTGAGAGCA GCTCAGGGAG AATAACAAAT TGGCTTGTTC TAAAGAACCT CACACCTCAG 
ATTGACGGCT CAACTCTGCG TACTCTGTGC ATGCAGCATG GCCCACTGAT AACATTCCAC 
CTGAACCTCC CACATGGAAA TGCTTTGGTC CGTTACAGTT CAAAAGAAGA GGTAGTGAAG 
GCACAAAAGT CTCTGCACAT GTGTGTATTG GGGAACACTA CTATTCTTGC TGAGTTTGCC 
AGTGAAGAGG AGATCAGCCG TTTCTTTGCA CAAAGTCAGT CTCTGACCCC TTCTCCCGGC 
TGGCAGTCTC TCGGGTCCAG CCAGAGCCGG CTGGGCTCCC TCGACTGTTC CCACTCGTTC 
TCCAGCCGGA CCGATCTCAA TCACTGGAAT GGTGCTGGGC TGTCGGGAAC TAACTGTGGA 
GACCTTCACG GCACTTCACT CTGGGGGACC CCACATTATT CCACAAGCCT GTGGGGTCCT 
CCCAGCAGCA GCGACCCCCG GGGAATTAGC AGCCCATCCC CCATTAATGC TTTTCTTTCT 
GTTGACCACC TGGGTGGGGG TGGAGAGTCC ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005621460.1
CDS	317..6112
Translation	MEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNPQQQQQPQPPQQPQQPPQQPPQPPQQQPQPAQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLSSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSPSDSSTNCKNAVVNDSPETEAWPSVPGSDPELASECMDADSASSSESERNITIMASGSTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSPGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLDNNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVGGSSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVMNGTSLSHLSNGEPKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGINGPMGTNFQVNTNKGGGVWESGATNSQNASWGSGNGANSGGSRRGWGTPAQNTGTNIPSVEWSKLPSNQHSNDSANGNGKKFTNGWKSTEEEDQASAASQTTEQNSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACTDKTSPTSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDAAATGMVKSNQWGNSKEEKSAWNDSQKSKQGWGDGQKSNQGWSVSASDNWGETSRSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVTSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPTKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSASAISNKEAGSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASNTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGTMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERSPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPSLLVKQQTPPSQQQPLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_005621403.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005621403.3

ORF Insert Sequence:

ATGGAAGAAA AGAAAAAGAA AAAAGACGAC AAGAAAAAGA AGGAAGCTGC TCAAAAGAAG 
GCCACTGAAC AAAAAATCAA AGTGCCAGAA CAGATAAAGC CCAGTGTAAG CCAGCCTCAG 
CCTGCCAACT CTAATAACGG CACTTCCACA GCAACCAGCA CTAATAATAA TGCCAAGCGA 
GCCACAGCCA ACAATCCGCA GCAGCAGCAG CAGCCACAGC CGCCACAGCA GCCACAGCAG 
CCGCCGCAGC AGCCACCGCA GCCGCCACAG CAGCAGCCGC AGCCAGCACA GGCCTTGCCT 
CGGTATCCTC GGGAAGTACC ACCACGATTT CGCCACCAGG AACACAAACA GCTTCTAAAG 
AGGGGTCAGC ATTTTCCTGT CATAGCAGCA AACCTGGGAT CTGCTGTTAA AGTGTTAAGC 
AGCCAGTCAG AAAGCAGTGC TTTAACAAAT CAACAGCCAC AAAATAACGG AGAGGTGCAG 
AACAGCAAAA ACCAGTCAGA TATAAACCAC AGTACTTCAG GATCCCATTA TGAAAATTCA 
CAGCGGGGAC CTGTGTCTTC TCCGAGTGAC TCTAGCACAA ATTGTAAGAA TGCTGTTGTA 
AACGACTCCC CTGAGACAGA AGCATGGCCC TCAGTCCCTG GCAGTGATCC AGAGTTGGCT 
TCAGAATGTA TGGATGCTGA TTCTGCCTCC AGTTCTGAAT CAGAGAGAAA CATCACTATC 
ATGGCTTCAG GGAGCACAGG TGGTGAAAAA GATGGCCTTC GGAATAGCAC TGGACTTGGT 
TCCCAAAACA AGTTTGTGGT TGGTAGCAGC AGCAATAATG TGGGCCATGG AAGTAGTCCT 
GGGCCATGGG GTTTTTCCCA TGGAGCCATA ATAAGCACAT GTCAGGTCTC TGTGGATGCT 
CCTGAAAGCA AATCAGAAAG TAGCAACAAT AGAATGAATG CTTGGGGCAC TGTAAGTTCT 
TCATCAAATG GAGGGTTAAA TCCAAGCACT TTGAATTCAG CTAGCAACCA TGGTGCCTGG 
CCAGTATTAG ACAACAACGG ACTTGCCCTC AAAGGGCCTG TAGGGAGCGG TAGTTCTGGC 
ATCAATATTC AGTGTAGTAC CATAGGCCAG ATGCCTAACA ATCAGAGTAT TAACTCTAAA 
GTGGGTGGTT CTTCCACCCA TGGTACCTGG GGAAGCCTTC AGGAAACTTG TGAATCTGAA 
GTAAGTGGTA CACAGAAGGT TTCATTCAGT GGTCAACCTC AGAATATTAC CACTGAGATG 
ACTGGACCAA ATAACACTAC TAACTTTATG ACCTCTAGTT TACCAAACTC CGGTTCAGTA 
CAGAATAACG AACTGCCTAG TAATACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT 
CAGATACAGG CTCCATCGGT TATGAATGGC ACTTCCCTTT CTCATCTTAG CAATGGAGAG 
CCAAAAAGTG GAGGCTCTTA CGGTACTACG TGGGGTGCCT ATGGTTCTAA TTACTCTGGA 
GACAAATGTT CGAGCCCTAA CGGCCAAGCT AATGGTGACA CTGTGAATGC AACTCTAATG 
CAGCCTGGCA TAAATGGGCC TATGGGCACT AACTTTCAAG TGAACACAAA TAAAGGAGGA 
GGTGTGTGGG AGTCTGGGGC AACGAATTCC CAGAATGCAT CGTGGGGAAG TGGAAATGGC 
GCAAATTCTG GAGGAAGTCG ACGAGGATGG GGAACTCCTG CACAAAACAC TGGCACTAAT 
ATACCCAGCG TGGAGTGGAG CAAACTGCCT AGCAATCAGC ATTCCAATGA CAGTGCAAAT 
GGCAATGGTA AGAAGTTTAC AAATGGATGG AAATCTACTG AGGAAGAGGA TCAGGCTTCT 
GCCGCATCTC AGACAACTGA GCAGAACAGT GTGTGGGCCA AAACAGGAGG TACAGTGGAG 
AGTGAAGGTA GTACAGAAAG CACTGGACGC CTCGAGGAGA AAGCCACTGG GGAAAATCAG 
AGTAGAGATC GGAGAAAAAT TGATCAGCAC ACATTACTCC AAAGCATCGT AAACAGAACT 
GACTTGGATC CACGTGTCCT CTCCAACTCT GGTTGGGGAC AGACTCCTAT TAAGCAGAAT 
ACTGCCTGGG ATACGGAAAC ATCACCTAGA GGGGAAAGAA AGACTGACAA TGGGACAGAG 
GCCTGGGGAA GCTCTGCAAC ACAGACTTTT AACTCAGGGG CATGTACAGA TAAGACTAGC 
CCTACTAGTA ATGATACCTC ATCTGTATCG GGGTGGGGAG ATCCCAAACC TGCTCTGAGG 
TGGGGAGATT CCAAAGGCTC AAGCTGCCAG GGGGGATGGG AAGATGATGC TGCTGCTACA 
GGAATGGTCA AGAGCAATCA GTGGGGGAAC AGCAAAGAAG AGAAGTCCGC ATGGAATGAT 
TCGCAAAAGA GCAAACAGGG ATGGGGTGAT GGACAGAAAT CAAACCAAGG GTGGTCCGTT 
TCTGCCAGTG ATAACTGGGG AGAAACGTCA AGGAGTAACC ATTGGGGTGA GGCTAATAAG 
AAATCTAGCT CAGGAGGTAG TGACAGTGAC AGGTCTGTTT CTGGTTGGAA TGAACTTGGT 
AAAACTAGTT CTTTTACTTG GGGAAATAAC ATAAATCCAA ATAATTCGTC AGGATGGGAT 
GAATCTTCTA AACCTAATCC TTCCCAGGGA TGGGGAGATC CTCCAAAGTC TAATCAGTCT 
CTAGGTTGGG GAGATTCGTC AAAGCCAGTC ACTTCTCCAG ACTGGAACAA GCAACAAGAC 
ATTGTCGGGT CTTGGGGAAT CCCACCAGCA ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG 
GGAGGACCTA TACCAGCCCC AACAAAAGAA GAAGAGCCCA CAGGCTGGGA GGAACCATCC 
CCAGAATCCA TACGTCGCAA AATGGAGATC GATGACGGAA CTTCAGCTTG GGGAGATCCG 
AGCAAATACA ACTACAAAAA TGTGAACATG TGGAACAAAA ATGTCCCAAA TGGCAGCAGC 
CGTTCAGACC AGCAAGCACA GGTACACCAG TTGCTACCAT CTGCAAGTGC CATCTCAAAC 
AAAGAGGCAG GCAGTGGCTC TGGCTGGGGT GAGCCCTGGG GGGAGCCTTC TACTCCAGCC 
ACAACTGTGG ATAATGGTAC TTCAGCATGG GGTAAACCCA TAGACAGTGG TCCCAGCTGG 
GGGGAGCCCA TTGCTGCAGC ATCCAACACA TCCACGTGGG GCTCCAGCTC TGTTGGTCCT 
CAAGCATTAA GCAAATCTGG GCCAAAATCT ATGCAAGATG GCTGGTGTGG TGATGATATG 
CCATTGCCTG GAAATCGCCC CACTGGCTGG GAAGAGGAAG AGGATGTAGA GATTGGAATG 
TGGAATAGTA ATTCATCTCA AGAGCTTAAC TCATCTTTAA ATTGGCCTCC ATATACAAAG 
AAAATGTCAT CGAAGGGTCT GAGTGGCAAA AAAAGGAGAA GGGAAAGGGG AACCATGAAA 
GGTGGAAACA AACAAGAAGA AGCATGGATA AATCCATTCG TTAAACAGTT TTCAAATATC 
AGTTTTTCGA GAGACTCACC CGAAGAAAAT GTACAGAGCA ATAAGATGGA TCTTTCCGGA 
GGAATGTTAC AGGACAAACG AATGGAGATA GATAAACATA GCCTAAATAT TGGTGATTAC 
AATCGAACGG TCGGGAAAGG CCCTGGTTCT CGGCCTCAGA TTTCCAAAGA GTCTTCCATG 
GAGCGCAGCC CTTACTTTGA TAAGGATGGC ATTGTAGCAG ATGAATCCCA AAACATGCAG 
TTTATGTCCA GTCAAAGCAT GAAGCTTCCC CCTTCAAATA GTGCACTACC TAACCAGGCC 
CTTGGCTCCA TAGCAGGGCT GGGTATGCAA AACTTGAATT CTGTTAGACA GAATGGCAAT 
CCCAGTATGT TTGGTGTTGG AAACACAGCA GCACAACCCC GGGGCATGCA GCAGCCTCCA 
GCACAACCTC TTAGTTCATC TCAGCCTAAT CTTCGTGCTC AAGTGCCTCC TCCATTACTC 
TCCCCTCAGG TTCCAGTTTC ATTGCTGAAG TATGCACCAA ACAACGGTGG CCTGAATCCG 
CTCTTTGGCC CTCAACAGGT AGCCATGCTG AACCAGCTAT CCCAACTAAA CCAGCTTTCT 
CAGATCTCCC AGTTACAGAG ATTGTTAGCG CAGCAGCAAA GGGCGCAGAG TCAGAGAAGC 
GTGCCTTCTG GGAACCGGCA GCAGCAAGAC CAGCAGGGTC GACCTCTTAG CGTGCAGCAG 
CAGATGATGC AGCAGTCCCG GCAACTCGAT CCCAGCCTGT TGGTGAAGCA GCAGACCCCG 
CCATCTCAGC AGCAGCCACT CCATCAGCCA GCCATGAAAT CCTTCCTCGA GAATGTCATG 
CCCCACACTA CACCTGAGCT GCAGAAAGGG CCATCACCAA TAAATGCTTT CAGCAACTTC 
CCGATAGGCT TGAACTCAAA CTTGAATGTA AATATGGATA TGAACAGTAT TAAAGAGCCA 
CAGTCAAGAC TAAGGAAGTG GACTACAGTG GACAGCATTT CTGTGAACAC ATCTTTGGAT 
CAAAACTCCA GCAAACATGG TGCTATTTCA AGTGGTTTCA GGCTGGAAGA GTCTCCATTT 
GTTCCCTATG ACTTTATGAA CAGCAGTACT TCTCCAGCCA GCCCTCCGGG TTCAATAGGA 
GATGGCTGGC CACGTGCCAA ATCGCCTAAC GGCTCTAGCA GTGTTAACTG GCCACCAGAA 
TTTCGCCCTG GTGAACCATG GAAAGGTTAT CCAAACATTG ACCCTGAAAC TGACCCTTAC 
GTCACTCCTG GCAGTGTCAT AAACAATCTT TCAATTAATA CTGTGCGGGA AGTTGACCAC 
CTCAGGGACA GGAACAGTGG GTCATCCTCA TCCTTGAACA CCACGCTGCC TTCAACTAGT 
GCCTGGTCAT CCATTCGTGC CTCCAACTAC AACGTTCCCC TCAGCAGTAC AGCACAAAGC 
ACTTCAGCCA GAAATAGTGA TTCCAAATTG ACATGGTCTC CTGGTTCAGT TACAAACACC 
TCTCTGGCTC ATGAGCTGTG GAAGGTCCCT TTACCACCTA AAAACATCAC TGCTCCGTCC 
CGCCCACCTC CGGGACTCAC TGGTCAGAAG CCACCCTTGT CTACGTGGGA TAACTCTCCC 
CTTCGTGTAG GTGGAGGATG GGGAAATTCT GACGCCAGGT ACACCCCAGG TTCCAGTTGG 
GGTGAGAGCA GCTCAGGGAG AATAACAAAT TGGCTTGTTC TAAAGAACCT CACACCTCAG 
ATTGACGGCT CAACTCTGCG TACTCTGTGC ATGCAGCATG GCCCACTGAT AACATTCCAC 
CTGAACCTCC CACATGGAAA TGCTTTGGTC CGTTACAGTT CAAAAGAAGA GGTAGTGAAG 
GCACAAAAGT CTCTGCACAT GTGTGTATTG GGGAACACTA CTATTCTTGC TGAGTTTGCC 
AGTGAAGAGG AGATCAGCCG TTTCTTTGCA CAAAGTCAGT CTCTGACCCC TTCTCCCGGC 
TGGCAGTCTC TCGGGTCCAG CCAGAGCCGG CTGGGCTCCC TCGACTGTTC CCACTCGTTC 
TCCAGCCGGA CCGATCTCAA TCACTGGAAT GGTGCTGGGC TGTCGGGAAC TAACTGTGGA 
GACCTTCACG GCACTTCACT CTGGGGGACC CCACATTATT CCACAAGCCT GTGGGGTCCT 
CCCAGCAGCA GCGACCCCCG GGGAATTAGC AGCCCATCCC CCATTAATGC TTTTCTTTCT 
GTTGACCACC TGGGTGGGGG TGGAGAGTCC ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_005621404.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005621461.1 Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X6, mRNA.

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CloneID	OCe07961
Clone ID Related Accession (Same CDS sequence)	XM_005621404.2 , XM_005621404.3
Accession Version	XM_005621404.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	trinucleotide repeat-containing gene 6A protein isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005621404.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACTAG TGGCAGAGCC AGGCCTAGAA GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG AGAATTGGAA GCTAAAGCTA CCAAAGACGT AGAAAGGACT 
CTTAGCAGGG ATTTAGTGCA AGAAGAAGAA CAGTTGATGG AAGAAAAGAA AAAGAAAAAA 
GACGACAAGA AAAAGAAGGA AGCTGCTCAA AAGAAGGCCA CTGAACAAAA AATCAAAGTG 
CCAGAACAGA TAAAGCCCAG TGTAAGCCAG CCTCAGCCTG CCAACTCTAA TAACGGCACT 
TCCACAGCAA CCAGCACTAA TAATAATGCC AAGCGAGCCA CAGCCAACAA TCCGCAGCAG 
CAGCAGCAGC CACAGCCGCC ACAGCAGCCA CAGCAGCCGC CGCAGCAGCC ACCGCAGCCG 
CCACAGCAGC AGCCGCAGCC AGCACAGGCC TTGCCTCGGT ATCCTCGGGA AGTACCACCA 
CGATTTCGCC ACCAGGAACA CAAACAGCTT CTAAAGAGGG GTCAGCATTT TCCTGTCATA 
GCAGCAAACC TGGGATCTGC TGTTAAAGTG TTAAGCAGCC AGTCAGAAAG CAGTGCTTTA 
ACAAATCAAC AGCCACAAAA TAACGGAGAG GTGCAGAACA GCAAAAACCA GTCAGATATA 
AACCACAGTA CTTCAGGATC CCATTATGAA AATTCACAGC GGGGACCTGT GTCTTCTCCG 
AGTGACTCTA GCACAAATTG TAAGAATGCT GTTGTAAACG ACTCCCCTGA GACAGAAGCA 
TGGCCCTCAG TCCCTGGCAG TGATCCAGAG TTGGCTTCAG AATGTATGGA TGCTGATTCT 
GCCTCCAGTT CTGAATCAGA GAGAAACATC ACTATCATGG CTTCAGGGAG CACAGGTGGT 
GAAAAAGATG GCCTTCGGAA TAGCACTGGA CTTGGTTCCC AAAACAAGTT TGTGGTTGGT 
AGCAGCAGCA ATAATGTGGG CCATGGAAGT AGTCCTGGGC CATGGGGTTT TTCCCATGGA 
GCCATAATAA GCACATGTCA GGTCTCTGTG GATGCTCCTG AAAGCAAATC AGAAAGTAGC 
AACAATAGAA TGAATGCTTG GGGCACTGTA AGTTCTTCAT CAAATGGAGG GTTAAATCCA 
AGCACTTTGA ATTCAGCTAG CAACCATGGT GCCTGGCCAG TATTAGACAA CAACGGACTT 
GCCCTCAAAG GGCCTGTAGG GAGCGGTAGT TCTGGCATCA ATATTCAGTG TAGTACCATA 
GGCCAGATGC CTAACAATCA GAGTATTAAC TCTAAAGTGG GTGGTTCTTC CACCCATGGT 
ACCTGGGGAA GCCTTCAGGA AACTTGTGAA TCTGAAGTAA GTGGTACACA GAAGGTTTCA 
TTCAGTGGTC AACCTCAGAA TATTACCACT GAGATGACTG GACCAAATAA CACTACTAAC 
TTTATGACCT CTAGTTTACC AAACTCCGGT TCAGTACAGA ATAACGAACT GCCTAGTAAT 
ACAGGGGCCT GGCGTGTGAG CACAATGAAT CATCCTCAGA TACAGGCTCC ATCGGTTATG 
AATGGCACTT CCCTTTCTCA TCTTAGCAAT GGAGAGCCAA AAAGTGGAGG CTCTTACGGT 
ACTACGTGGG GTGCCTATGG TTCTAATTAC TCTGGAGACA AATGTTCGAG CCCTAACGGC 
CAAGCTAATG GTGACACTGT GAATGCAACT CTAATGCAGC CTGGCATAAA TGGGCCTATG 
GGCACTAACT TTCAAGTGAA CACAAATAAA GGAGGAGGTG TGTGGGAGTC TGGGGCAACG 
AATTCCCAGA ATGCATCGTG GGGAAGTGGA AATGGCGCAA ATTCTGGAGG AAGTCGACGA 
GGATGGGGAA CTCCTGCACA AAACACTGGC ACTAATATAC CCAGCGTGGA GTGGAGCAAA 
CTGCCTAGCA ATCAGCATTC CAATGACAGT GCAAATGGCA ATGGTAAGAA GTTTACAAAT 
GGATGGAAAT CTACTGAGGA AGAGGATCAG GCTTCTGCCG CATCTCAGAC AACTGAGCAG 
AACAGTGTGT GGGCCAAAAC AGGAGGTACA GTGGAGAGTG AAGGTAGTAC AGAAAGCACT 
GGACGCCTCG AGGAGAAAGC CACTGGGGAA AATCAGAGTA GAGATCGGAG AAAAATTGAT 
CAGCACACAT TACTCCAAAG CATCGTAAAC AGAACTGACT TGGATCCACG TGTCCTCTCC 
AACTCTGGTT GGGGACAGAC TCCTATTAAG CAGAATACTG CCTGGGATAC GGAAACATCA 
CCTAGAGGGG AAAGAAAGAC TGACAATGGG ACAGAGGCCT GGGGAAGCTC TGCAACACAG 
ACTTTTAACT CAGGGGCATG TACAGATAAG ACTAGCCCTA CTAGTAATGA TACCTCATCT 
GTATCGGGGT GGGGAGATCC CAAACCTGCT CTGAGGTGGG GAGATTCCAA AGGCTCAAGC 
TGCCAGGGGG GATGGGAAGA TGATGCTGCT GCTACAGGAA TGGTCAAGAG CAATCAGTGG 
GGGAACAGCA AAGAAGAGAA GTCCGCATGG AATGATTCGC AAAAGAGCAA ACAGGGATGG 
GGTGATGGAC AGAAATCAAA CCAAGGGTGG TCCGTTTCTG CCAGTGATAA CTGGGGAGAA 
ACGTCAAGGA GTAACCATTG GGGTGAGGCT AATAAGAAAT CTAGCTCAGG AGGTAGTGAC 
AGTGACAGGT CTGTTTCTGG TTGGAATGAA CTTGGTAAAA CTAGTTCTTT TACTTGGGGA 
AATAACATAA ATCCAAATAA TTCGTCAGGA TGGGATGAAT CTTCTAAACC TAATCCTTCC 
CAGGGATGGG GAGATCCTCC AAAGTCTAAT CAGTCTCTAG GTTGGGGAGA TTCGTCAAAG 
CCAGTCACTT CTCCAGACTG GAACAAGCAA CAAGACATTG TCGGGTCTTG GGGAATCCCA 
CCAGCAACAG GCAAACCTCC TGGTACAGGC TGGCTGGGAG GACCTATACC AGCCCCAACA 
AAAGAAGAAG AGCCCACAGG CTGGGAGGAA CCATCCCCAG AATCCATACG TCGCAAAATG 
GAGATCGATG ACGGAACTTC AGCTTGGGGA GATCCGAGCA AATACAACTA CAAAAATGTG 
AACATGTGGA ACAAAAATGT CCCAAATGGC AGCAGCCGTT CAGACCAGCA AGCACAGGTA 
CACCAGTTGC TACCATCTGC AAGTGCCATC TCAAACAAAG AGGCAGGCAG TGGCTCTGGC 
TGGGGTGAGC CCTGGGGGGA GCCTTCTACT CCAGCCACAA CTGTGGATAA TGGTACTTCA 
GCATGGGGTA AACCCATAGA CAGTGGTCCC AGCTGGGGGG AGCCCATTGC TGCAGCATCC 
AACACATCCA CGTGGGGCTC CAGCTCTGTT GGTCCTCAAG CATTAAGCAA ATCTGGGCCA 
AAATCTATGC AAGATGGCTG GTGTGGTGAT GATATGCCAT TGCCTGGAAA TCGCCCCACT 
GGCTGGGAAG AGGAAGAGGA TGTAGAGATT GGAATGTGGA ATAGTAATTC ATCTCAAGAG 
CTTAACTCAT CTTTAAATTG GCCTCCATAT ACAAAGAAAA TGTCATCGAA GGGTCTGAGT 
GGCAAAAAAA GGAGAAGGGA AAGGGGAACC ATGAAAGGTG GAAACAAACA AGAAGAAGCA 
TGGATAAATC CATTCGTTAA ACAGTTTTCA AATATCAGTT TTTCGAGAGA CTCACCCGAA 
GAAAATGTAC AGAGCAATAA GATGGATCTT TCCGGAGGAA TGTTACAGGA CAAACGAATG 
GAGATAGATA AACATAGCCT AAATATTGGT GATTACAATC GAACGGTCGG GAAAGGCCCT 
GGTTCTCGGC CTCAGATTTC CAAAGAGTCT TCCATGGAGC GCAGCCCTTA CTTTGATAAG 
GATGGCATTG TAGCAGATGA ATCCCAAAAC ATGCAGTTTA TGTCCAGTCA AAGCATGAAG 
CTTCCCCCTT CAAATAGTGC ACTACCTAAC CAGGCCCTTG GCTCCATAGC AGGGCTGGGT 
ATGCAAAACT TGAATTCTGT TAGACAGAAT GGCAATCCCA GTATGTTTGG TGTTGGAAAC 
ACAGCAGCAC AACCCCGGGG CATGCAGCAG CCTCCAGCAC AACCTCTTAG TTCATCTCAG 
CCTAATCTTC GTGCTCAAGT GCCTCCTCCA TTACTCTCCC CTCAGGTTCC AGTTTCATTG 
CTGAAGTATG CACCAAACAA CGGTGGCCTG AATCCGCTCT TTGGCCCTCA ACAGGTAGCC 
ATGCTGAACC AGCTATCCCA ACTAAACCAG CTTTCTCAGA TCTCCCAGTT ACAGAGATTG 
TTAGCGCAGC AGCAAAGGGC GCAGAGTCAG AGAAGCGTGC CTTCTGGGAA CCGGCAGCAG 
CAAGACCAGC AGGGTCGACC TCTTAGCGTG CAGCAGCAGA TGATGCAGCA GTCCCGGCAA 
CTCGATCCCA GCCTGTTGGT GAAGCAGCAG ACCCCGCCAT CTCAGCAGCA GCCACTCCAT 
CAGCCAGCCA TGAAATCCTT CCTCGAGAAT GTCATGCCCC ACACTACACC TGAGCTGCAG 
AAAGGGCCAT CACCAATAAA TGCTTTCAGC AACTTCCCGA TAGGCTTGAA CTCAAACTTG 
AATGTAAATA TGGATATGAA CAGTATTAAA GAGCCACAGT CAAGACTAAG GAAGTGGACT 
ACAGTGGACA GCATTTCTGT GAACACATCT TTGGATCAAA ACTCCAGCAA ACATGGTGCT 
ATTTCAAGTG GTTTCAGGCT GGAAGAGTCT CCATTTGTTC CCTATGACTT TATGAACAGC 
AGTACTTCTC CAGCCAGCCC TCCGGGTTCA ATAGGAGATG GCTGGCCACG TGCCAAATCG 
CCTAACGGCT CTAGCAGTGT TAACTGGCCA CCAGAATTTC GCCCTGGTGA ACCATGGAAA 
GGTTATCCAA ACATTGACCC TGAAACTGAC CCTTACGTCA CTCCTGGCAG TGTCATAAAC 
AATCTTTCAA TTAATACTGT GCGGGAAGTT GACCACCTCA GGGACAGGAA CAGTGGGTCA 
TCCTCATCCT TGAACACCAC GCTGCCTTCA ACTAGTGCCT GGTCATCCAT TCGTGCCTCC 
AACTACAACG TTCCCCTCAG CAGTACAGCA CAAAGCACTT CAGGTTCCAG TTGGGGTGAG 
AGCAGCTCAG GGAGAATAAC AAATTGGCTT GTTCTAAAGA ACCTCACACC TCAGATTGAC 
GGCTCAACTC TGCGTACTCT GTGCATGCAG CATGGCCCAC TGATAACATT CCACCTGAAC 
CTCCCACATG GAAATGCTTT GGTCCGTTAC AGTTCAAAAG AAGAGGTAGT GAAGGCACAA 
AAGTCTCTGC ACATGTGTGT ATTGGGGAAC ACTACTATTC TTGCTGAGTT TGCCAGTGAA 
GAGGAGATCA GCCGTTTCTT TGCACAAAGT CAGTCTCTGA CCCCTTCTCC CGGCTGGCAG 
TCTCTCGGGT CCAGCCAGAG CCGGCTGGGC TCCCTCGACT GTTCCCACTC GTTCTCCAGC 
CGGACCGATC TCAATCACTG GAATGGTGCT GGGCTGTCGG GAACTAACTG TGGAGACCTT 
CACGGCACTT CACTCTGGGG GACCCCACAT TATTCCACAA GCCTGTGGGG TCCTCCCAGC 
AGCAGCGACC CCCGGGGAAT TAGCAGCCCA TCCCCCATTA ATGCTTTTCT TTCTGTTGAC 
CACCTGGGTG GGGGTGGAGA GTCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005621461.1
CDS	284..6013
Translation	MQLVAEPGLEARCPGSQDIPLPEEWNRELEAKATKDVERTLSRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNPQQQQQPQPPQQPQQPPQQPPQPPQQQPQPAQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLSSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSPSDSSTNCKNAVVNDSPETEAWPSVPGSDPELASECMDADSASSSESERNITIMASGSTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSPGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLDNNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVGGSSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVMNGTSLSHLSNGEPKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGINGPMGTNFQVNTNKGGGVWESGATNSQNASWGSGNGANSGGSRRGWGTPAQNTGTNIPSVEWSKLPSNQHSNDSANGNGKKFTNGWKSTEEEDQASAASQTTEQNSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACTDKTSPTSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDAAATGMVKSNQWGNSKEEKSAWNDSQKSKQGWGDGQKSNQGWSVSASDNWGETSRSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVTSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPTKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSASAISNKEAGSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASNTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGTMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERSPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPSLLVKQQTPPSQQQPLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_005621404.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005621404.2

ORF Insert Sequence:

ATGCAACTAG TGGCAGAGCC AGGCCTAGAA GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG AGAATTGGAA GCTAAAGCTA CCAAAGACGT AGAAAGGACT 
CTTAGCAGGG ATTTAGTGCA AGAAGAAGAA CAGTTGATGG AAGAAAAGAA AAAGAAAAAA 
GACGACAAGA AAAAGAAGGA AGCTGCTCAA AAGAAGGCCA CTGAACAAAA AATCAAAGTG 
CCAGAACAGA TAAAGCCCAG TGTAAGCCAG CCTCAGCCTG CCAACTCTAA TAACGGCACT 
TCCACAGCAA CCAGCACTAA TAATAATGCC AAGCGAGCCA CAGCCAACAA TCCGCAGCAG 
CAGCAGCAGC CACAGCCGCC ACAGCAGCCA CAGCAGCCGC CGCAGCAGCC ACCGCAGCCG 
CCACAGCAGC AGCCGCAGCC AGCACAGGCC TTGCCTCGGT ATCCTCGGGA AGTACCACCA 
CGATTTCGCC ACCAGGAACA CAAACAGCTT CTAAAGAGGG GTCAGCATTT TCCTGTCATA 
GCAGCAAACC TGGGATCTGC TGTTAAAGTG TTAAGCAGCC AGTCAGAAAG CAGTGCTTTA 
ACAAATCAAC AGCCACAAAA TAACGGAGAG GTGCAGAACA GCAAAAACCA GTCAGATATA 
AACCACAGTA CTTCAGGATC CCATTATGAA AATTCACAGC GGGGACCTGT GTCTTCTCCG 
AGTGACTCTA GCACAAATTG TAAGAATGCT GTTGTAAACG ACTCCCCTGA GACAGAAGCA 
TGGCCCTCAG TCCCTGGCAG TGATCCAGAG TTGGCTTCAG AATGTATGGA TGCTGATTCT 
GCCTCCAGTT CTGAATCAGA GAGAAACATC ACTATCATGG CTTCAGGGAG CACAGGTGGT 
GAAAAAGATG GCCTTCGGAA TAGCACTGGA CTTGGTTCCC AAAACAAGTT TGTGGTTGGT 
AGCAGCAGCA ATAATGTGGG CCATGGAAGT AGTCCTGGGC CATGGGGTTT TTCCCATGGA 
GCCATAATAA GCACATGTCA GGTCTCTGTG GATGCTCCTG AAAGCAAATC AGAAAGTAGC 
AACAATAGAA TGAATGCTTG GGGCACTGTA AGTTCTTCAT CAAATGGAGG GTTAAATCCA 
AGCACTTTGA ATTCAGCTAG CAACCATGGT GCCTGGCCAG TATTAGACAA CAACGGACTT 
GCCCTCAAAG GGCCTGTAGG GAGCGGTAGT TCTGGCATCA ATATTCAGTG TAGTACCATA 
GGCCAGATGC CTAACAATCA GAGTATTAAC TCTAAAGTGG GTGGTTCTTC CACCCATGGT 
ACCTGGGGAA GCCTTCAGGA AACTTGTGAA TCTGAAGTAA GTGGTACACA GAAGGTTTCA 
TTCAGTGGTC AACCTCAGAA TATTACCACT GAGATGACTG GACCAAATAA CACTACTAAC 
TTTATGACCT CTAGTTTACC AAACTCCGGT TCAGTACAGA ATAACGAACT GCCTAGTAAT 
ACAGGGGCCT GGCGTGTGAG CACAATGAAT CATCCTCAGA TACAGGCTCC ATCGGTTATG 
AATGGCACTT CCCTTTCTCA TCTTAGCAAT GGAGAGCCAA AAAGTGGAGG CTCTTACGGT 
ACTACGTGGG GTGCCTATGG TTCTAATTAC TCTGGAGACA AATGTTCGAG CCCTAACGGC 
CAAGCTAATG GTGACACTGT GAATGCAACT CTAATGCAGC CTGGCATAAA TGGGCCTATG 
GGCACTAACT TTCAAGTGAA CACAAATAAA GGAGGAGGTG TGTGGGAGTC TGGGGCAACG 
AATTCCCAGA ATGCATCGTG GGGAAGTGGA AATGGCGCAA ATTCTGGAGG AAGTCGACGA 
GGATGGGGAA CTCCTGCACA AAACACTGGC ACTAATATAC CCAGCGTGGA GTGGAGCAAA 
CTGCCTAGCA ATCAGCATTC CAATGACAGT GCAAATGGCA ATGGTAAGAA GTTTACAAAT 
GGATGGAAAT CTACTGAGGA AGAGGATCAG GCTTCTGCCG CATCTCAGAC AACTGAGCAG 
AACAGTGTGT GGGCCAAAAC AGGAGGTACA GTGGAGAGTG AAGGTAGTAC AGAAAGCACT 
GGACGCCTCG AGGAGAAAGC CACTGGGGAA AATCAGAGTA GAGATCGGAG AAAAATTGAT 
CAGCACACAT TACTCCAAAG CATCGTAAAC AGAACTGACT TGGATCCACG TGTCCTCTCC 
AACTCTGGTT GGGGACAGAC TCCTATTAAG CAGAATACTG CCTGGGATAC GGAAACATCA 
CCTAGAGGGG AAAGAAAGAC TGACAATGGG ACAGAGGCCT GGGGAAGCTC TGCAACACAG 
ACTTTTAACT CAGGGGCATG TACAGATAAG ACTAGCCCTA CTAGTAATGA TACCTCATCT 
GTATCGGGGT GGGGAGATCC CAAACCTGCT CTGAGGTGGG GAGATTCCAA AGGCTCAAGC 
TGCCAGGGGG GATGGGAAGA TGATGCTGCT GCTACAGGAA TGGTCAAGAG CAATCAGTGG 
GGGAACAGCA AAGAAGAGAA GTCCGCATGG AATGATTCGC AAAAGAGCAA ACAGGGATGG 
GGTGATGGAC AGAAATCAAA CCAAGGGTGG TCCGTTTCTG CCAGTGATAA CTGGGGAGAA 
ACGTCAAGGA GTAACCATTG GGGTGAGGCT AATAAGAAAT CTAGCTCAGG AGGTAGTGAC 
AGTGACAGGT CTGTTTCTGG TTGGAATGAA CTTGGTAAAA CTAGTTCTTT TACTTGGGGA 
AATAACATAA ATCCAAATAA TTCGTCAGGA TGGGATGAAT CTTCTAAACC TAATCCTTCC 
CAGGGATGGG GAGATCCTCC AAAGTCTAAT CAGTCTCTAG GTTGGGGAGA TTCGTCAAAG 
CCAGTCACTT CTCCAGACTG GAACAAGCAA CAAGACATTG TCGGGTCTTG GGGAATCCCA 
CCAGCAACAG GCAAACCTCC TGGTACAGGC TGGCTGGGAG GACCTATACC AGCCCCAACA 
AAAGAAGAAG AGCCCACAGG CTGGGAGGAA CCATCCCCAG AATCCATACG TCGCAAAATG 
GAGATCGATG ACGGAACTTC AGCTTGGGGA GATCCGAGCA AATACAACTA CAAAAATGTG 
AACATGTGGA ACAAAAATGT CCCAAATGGC AGCAGCCGTT CAGACCAGCA AGCACAGGTA 
CACCAGTTGC TACCATCTGC AAGTGCCATC TCAAACAAAG AGGCAGGCAG TGGCTCTGGC 
TGGGGTGAGC CCTGGGGGGA GCCTTCTACT CCAGCCACAA CTGTGGATAA TGGTACTTCA 
GCATGGGGTA AACCCATAGA CAGTGGTCCC AGCTGGGGGG AGCCCATTGC TGCAGCATCC 
AACACATCCA CGTGGGGCTC CAGCTCTGTT GGTCCTCAAG CATTAAGCAA ATCTGGGCCA 
AAATCTATGC AAGATGGCTG GTGTGGTGAT GATATGCCAT TGCCTGGAAA TCGCCCCACT 
GGCTGGGAAG AGGAAGAGGA TGTAGAGATT GGAATGTGGA ATAGTAATTC ATCTCAAGAG 
CTTAACTCAT CTTTAAATTG GCCTCCATAT ACAAAGAAAA TGTCATCGAA GGGTCTGAGT 
GGCAAAAAAA GGAGAAGGGA AAGGGGAACC ATGAAAGGTG GAAACAAACA AGAAGAAGCA 
TGGATAAATC CATTCGTTAA ACAGTTTTCA AATATCAGTT TTTCGAGAGA CTCACCCGAA 
GAAAATGTAC AGAGCAATAA GATGGATCTT TCCGGAGGAA TGTTACAGGA CAAACGAATG 
GAGATAGATA AACATAGCCT AAATATTGGT GATTACAATC GAACGGTCGG GAAAGGCCCT 
GGTTCTCGGC CTCAGATTTC CAAAGAGTCT TCCATGGAGC GCAGCCCTTA CTTTGATAAG 
GATGGCATTG TAGCAGATGA ATCCCAAAAC ATGCAGTTTA TGTCCAGTCA AAGCATGAAG 
CTTCCCCCTT CAAATAGTGC ACTACCTAAC CAGGCCCTTG GCTCCATAGC AGGGCTGGGT 
ATGCAAAACT TGAATTCTGT TAGACAGAAT GGCAATCCCA GTATGTTTGG TGTTGGAAAC 
ACAGCAGCAC AACCCCGGGG CATGCAGCAG CCTCCAGCAC AACCTCTTAG TTCATCTCAG 
CCTAATCTTC GTGCTCAAGT GCCTCCTCCA TTACTCTCCC CTCAGGTTCC AGTTTCATTG 
CTGAAGTATG CACCAAACAA CGGTGGCCTG AATCCGCTCT TTGGCCCTCA ACAGGTAGCC 
ATGCTGAACC AGCTATCCCA ACTAAACCAG CTTTCTCAGA TCTCCCAGTT ACAGAGATTG 
TTAGCGCAGC AGCAAAGGGC GCAGAGTCAG AGAAGCGTGC CTTCTGGGAA CCGGCAGCAG 
CAAGACCAGC AGGGTCGACC TCTTAGCGTG CAGCAGCAGA TGATGCAGCA GTCCCGGCAA 
CTCGATCCCA GCCTGTTGGT GAAGCAGCAG ACCCCGCCAT CTCAGCAGCA GCCACTCCAT 
CAGCCAGCCA TGAAATCCTT CCTCGAGAAT GTCATGCCCC ACACTACACC TGAGCTGCAG 
AAAGGGCCAT CACCAATAAA TGCTTTCAGC AACTTCCCGA TAGGCTTGAA CTCAAACTTG 
AATGTAAATA TGGATATGAA CAGTATTAAA GAGCCACAGT CAAGACTAAG GAAGTGGACT 
ACAGTGGACA GCATTTCTGT GAACACATCT TTGGATCAAA ACTCCAGCAA ACATGGTGCT 
ATTTCAAGTG GTTTCAGGCT GGAAGAGTCT CCATTTGTTC CCTATGACTT TATGAACAGC 
AGTACTTCTC CAGCCAGCCC TCCGGGTTCA ATAGGAGATG GCTGGCCACG TGCCAAATCG 
CCTAACGGCT CTAGCAGTGT TAACTGGCCA CCAGAATTTC GCCCTGGTGA ACCATGGAAA 
GGTTATCCAA ACATTGACCC TGAAACTGAC CCTTACGTCA CTCCTGGCAG TGTCATAAAC 
AATCTTTCAA TTAATACTGT GCGGGAAGTT GACCACCTCA GGGACAGGAA CAGTGGGTCA 
TCCTCATCCT TGAACACCAC GCTGCCTTCA ACTAGTGCCT GGTCATCCAT TCGTGCCTCC 
AACTACAACG TTCCCCTCAG CAGTACAGCA CAAAGCACTT CAGGTTCCAG TTGGGGTGAG 
AGCAGCTCAG GGAGAATAAC AAATTGGCTT GTTCTAAAGA ACCTCACACC TCAGATTGAC 
GGCTCAACTC TGCGTACTCT GTGCATGCAG CATGGCCCAC TGATAACATT CCACCTGAAC 
CTCCCACATG GAAATGCTTT GGTCCGTTAC AGTTCAAAAG AAGAGGTAGT GAAGGCACAA 
AAGTCTCTGC ACATGTGTGT ATTGGGGAAC ACTACTATTC TTGCTGAGTT TGCCAGTGAA 
GAGGAGATCA GCCGTTTCTT TGCACAAAGT CAGTCTCTGA CCCCTTCTCC CGGCTGGCAG 
TCTCTCGGGT CCAGCCAGAG CCGGCTGGGC TCCCTCGACT GTTCCCACTC GTTCTCCAGC 
CGGACCGATC TCAATCACTG GAATGGTGCT GGGCTGTCGG GAACTAACTG TGGAGACCTT 
CACGGCACTT CACTCTGGGG GACCCCACAT TATTCCACAA GCCTGTGGGG TCCTCCCAGC 
AGCAGCGACC CCCGGGGAAT TAGCAGCCCA TCCCCCATTA ATGCTTTTCT TTCTGTTGAC 
CACCTGGGTG GGGGTGGAGA GTCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_005621404.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005621461.1 Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OCe07961
Clone ID Related Accession (Same CDS sequence)	XM_005621404.2 , XM_005621404.3
Accession Version	XM_005621404.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	trinucleotide repeat-containing gene 6A protein isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005621404.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACTAG TGGCAGAGCC AGGCCTAGAA GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG AGAATTGGAA GCTAAAGCTA CCAAAGACGT AGAAAGGACT 
CTTAGCAGGG ATTTAGTGCA AGAAGAAGAA CAGTTGATGG AAGAAAAGAA AAAGAAAAAA 
GACGACAAGA AAAAGAAGGA AGCTGCTCAA AAGAAGGCCA CTGAACAAAA AATCAAAGTG 
CCAGAACAGA TAAAGCCCAG TGTAAGCCAG CCTCAGCCTG CCAACTCTAA TAACGGCACT 
TCCACAGCAA CCAGCACTAA TAATAATGCC AAGCGAGCCA CAGCCAACAA TCCGCAGCAG 
CAGCAGCAGC CACAGCCGCC ACAGCAGCCA CAGCAGCCGC CGCAGCAGCC ACCGCAGCCG 
CCACAGCAGC AGCCGCAGCC AGCACAGGCC TTGCCTCGGT ATCCTCGGGA AGTACCACCA 
CGATTTCGCC ACCAGGAACA CAAACAGCTT CTAAAGAGGG GTCAGCATTT TCCTGTCATA 
GCAGCAAACC TGGGATCTGC TGTTAAAGTG TTAAGCAGCC AGTCAGAAAG CAGTGCTTTA 
ACAAATCAAC AGCCACAAAA TAACGGAGAG GTGCAGAACA GCAAAAACCA GTCAGATATA 
AACCACAGTA CTTCAGGATC CCATTATGAA AATTCACAGC GGGGACCTGT GTCTTCTCCG 
AGTGACTCTA GCACAAATTG TAAGAATGCT GTTGTAAACG ACTCCCCTGA GACAGAAGCA 
TGGCCCTCAG TCCCTGGCAG TGATCCAGAG TTGGCTTCAG AATGTATGGA TGCTGATTCT 
GCCTCCAGTT CTGAATCAGA GAGAAACATC ACTATCATGG CTTCAGGGAG CACAGGTGGT 
GAAAAAGATG GCCTTCGGAA TAGCACTGGA CTTGGTTCCC AAAACAAGTT TGTGGTTGGT 
AGCAGCAGCA ATAATGTGGG CCATGGAAGT AGTCCTGGGC CATGGGGTTT TTCCCATGGA 
GCCATAATAA GCACATGTCA GGTCTCTGTG GATGCTCCTG AAAGCAAATC AGAAAGTAGC 
AACAATAGAA TGAATGCTTG GGGCACTGTA AGTTCTTCAT CAAATGGAGG GTTAAATCCA 
AGCACTTTGA ATTCAGCTAG CAACCATGGT GCCTGGCCAG TATTAGACAA CAACGGACTT 
GCCCTCAAAG GGCCTGTAGG GAGCGGTAGT TCTGGCATCA ATATTCAGTG TAGTACCATA 
GGCCAGATGC CTAACAATCA GAGTATTAAC TCTAAAGTGG GTGGTTCTTC CACCCATGGT 
ACCTGGGGAA GCCTTCAGGA AACTTGTGAA TCTGAAGTAA GTGGTACACA GAAGGTTTCA 
TTCAGTGGTC AACCTCAGAA TATTACCACT GAGATGACTG GACCAAATAA CACTACTAAC 
TTTATGACCT CTAGTTTACC AAACTCCGGT TCAGTACAGA ATAACGAACT GCCTAGTAAT 
ACAGGGGCCT GGCGTGTGAG CACAATGAAT CATCCTCAGA TACAGGCTCC ATCGGTTATG 
AATGGCACTT CCCTTTCTCA TCTTAGCAAT GGAGAGCCAA AAAGTGGAGG CTCTTACGGT 
ACTACGTGGG GTGCCTATGG TTCTAATTAC TCTGGAGACA AATGTTCGAG CCCTAACGGC 
CAAGCTAATG GTGACACTGT GAATGCAACT CTAATGCAGC CTGGCATAAA TGGGCCTATG 
GGCACTAACT TTCAAGTGAA CACAAATAAA GGAGGAGGTG TGTGGGAGTC TGGGGCAACG 
AATTCCCAGA ATGCATCGTG GGGAAGTGGA AATGGCGCAA ATTCTGGAGG AAGTCGACGA 
GGATGGGGAA CTCCTGCACA AAACACTGGC ACTAATATAC CCAGCGTGGA GTGGAGCAAA 
CTGCCTAGCA ATCAGCATTC CAATGACAGT GCAAATGGCA ATGGTAAGAA GTTTACAAAT 
GGATGGAAAT CTACTGAGGA AGAGGATCAG GCTTCTGCCG CATCTCAGAC AACTGAGCAG 
AACAGTGTGT GGGCCAAAAC AGGAGGTACA GTGGAGAGTG AAGGTAGTAC AGAAAGCACT 
GGACGCCTCG AGGAGAAAGC CACTGGGGAA AATCAGAGTA GAGATCGGAG AAAAATTGAT 
CAGCACACAT TACTCCAAAG CATCGTAAAC AGAACTGACT TGGATCCACG TGTCCTCTCC 
AACTCTGGTT GGGGACAGAC TCCTATTAAG CAGAATACTG CCTGGGATAC GGAAACATCA 
CCTAGAGGGG AAAGAAAGAC TGACAATGGG ACAGAGGCCT GGGGAAGCTC TGCAACACAG 
ACTTTTAACT CAGGGGCATG TACAGATAAG ACTAGCCCTA CTAGTAATGA TACCTCATCT 
GTATCGGGGT GGGGAGATCC CAAACCTGCT CTGAGGTGGG GAGATTCCAA AGGCTCAAGC 
TGCCAGGGGG GATGGGAAGA TGATGCTGCT GCTACAGGAA TGGTCAAGAG CAATCAGTGG 
GGGAACAGCA AAGAAGAGAA GTCCGCATGG AATGATTCGC AAAAGAGCAA ACAGGGATGG 
GGTGATGGAC AGAAATCAAA CCAAGGGTGG TCCGTTTCTG CCAGTGATAA CTGGGGAGAA 
ACGTCAAGGA GTAACCATTG GGGTGAGGCT AATAAGAAAT CTAGCTCAGG AGGTAGTGAC 
AGTGACAGGT CTGTTTCTGG TTGGAATGAA CTTGGTAAAA CTAGTTCTTT TACTTGGGGA 
AATAACATAA ATCCAAATAA TTCGTCAGGA TGGGATGAAT CTTCTAAACC TAATCCTTCC 
CAGGGATGGG GAGATCCTCC AAAGTCTAAT CAGTCTCTAG GTTGGGGAGA TTCGTCAAAG 
CCAGTCACTT CTCCAGACTG GAACAAGCAA CAAGACATTG TCGGGTCTTG GGGAATCCCA 
CCAGCAACAG GCAAACCTCC TGGTACAGGC TGGCTGGGAG GACCTATACC AGCCCCAACA 
AAAGAAGAAG AGCCCACAGG CTGGGAGGAA CCATCCCCAG AATCCATACG TCGCAAAATG 
GAGATCGATG ACGGAACTTC AGCTTGGGGA GATCCGAGCA AATACAACTA CAAAAATGTG 
AACATGTGGA ACAAAAATGT CCCAAATGGC AGCAGCCGTT CAGACCAGCA AGCACAGGTA 
CACCAGTTGC TACCATCTGC AAGTGCCATC TCAAACAAAG AGGCAGGCAG TGGCTCTGGC 
TGGGGTGAGC CCTGGGGGGA GCCTTCTACT CCAGCCACAA CTGTGGATAA TGGTACTTCA 
GCATGGGGTA AACCCATAGA CAGTGGTCCC AGCTGGGGGG AGCCCATTGC TGCAGCATCC 
AACACATCCA CGTGGGGCTC CAGCTCTGTT GGTCCTCAAG CATTAAGCAA ATCTGGGCCA 
AAATCTATGC AAGATGGCTG GTGTGGTGAT GATATGCCAT TGCCTGGAAA TCGCCCCACT 
GGCTGGGAAG AGGAAGAGGA TGTAGAGATT GGAATGTGGA ATAGTAATTC ATCTCAAGAG 
CTTAACTCAT CTTTAAATTG GCCTCCATAT ACAAAGAAAA TGTCATCGAA GGGTCTGAGT 
GGCAAAAAAA GGAGAAGGGA AAGGGGAACC ATGAAAGGTG GAAACAAACA AGAAGAAGCA 
TGGATAAATC CATTCGTTAA ACAGTTTTCA AATATCAGTT TTTCGAGAGA CTCACCCGAA 
GAAAATGTAC AGAGCAATAA GATGGATCTT TCCGGAGGAA TGTTACAGGA CAAACGAATG 
GAGATAGATA AACATAGCCT AAATATTGGT GATTACAATC GAACGGTCGG GAAAGGCCCT 
GGTTCTCGGC CTCAGATTTC CAAAGAGTCT TCCATGGAGC GCAGCCCTTA CTTTGATAAG 
GATGGCATTG TAGCAGATGA ATCCCAAAAC ATGCAGTTTA TGTCCAGTCA AAGCATGAAG 
CTTCCCCCTT CAAATAGTGC ACTACCTAAC CAGGCCCTTG GCTCCATAGC AGGGCTGGGT 
ATGCAAAACT TGAATTCTGT TAGACAGAAT GGCAATCCCA GTATGTTTGG TGTTGGAAAC 
ACAGCAGCAC AACCCCGGGG CATGCAGCAG CCTCCAGCAC AACCTCTTAG TTCATCTCAG 
CCTAATCTTC GTGCTCAAGT GCCTCCTCCA TTACTCTCCC CTCAGGTTCC AGTTTCATTG 
CTGAAGTATG CACCAAACAA CGGTGGCCTG AATCCGCTCT TTGGCCCTCA ACAGGTAGCC 
ATGCTGAACC AGCTATCCCA ACTAAACCAG CTTTCTCAGA TCTCCCAGTT ACAGAGATTG 
TTAGCGCAGC AGCAAAGGGC GCAGAGTCAG AGAAGCGTGC CTTCTGGGAA CCGGCAGCAG 
CAAGACCAGC AGGGTCGACC TCTTAGCGTG CAGCAGCAGA TGATGCAGCA GTCCCGGCAA 
CTCGATCCCA GCCTGTTGGT GAAGCAGCAG ACCCCGCCAT CTCAGCAGCA GCCACTCCAT 
CAGCCAGCCA TGAAATCCTT CCTCGAGAAT GTCATGCCCC ACACTACACC TGAGCTGCAG 
AAAGGGCCAT CACCAATAAA TGCTTTCAGC AACTTCCCGA TAGGCTTGAA CTCAAACTTG 
AATGTAAATA TGGATATGAA CAGTATTAAA GAGCCACAGT CAAGACTAAG GAAGTGGACT 
ACAGTGGACA GCATTTCTGT GAACACATCT TTGGATCAAA ACTCCAGCAA ACATGGTGCT 
ATTTCAAGTG GTTTCAGGCT GGAAGAGTCT CCATTTGTTC CCTATGACTT TATGAACAGC 
AGTACTTCTC CAGCCAGCCC TCCGGGTTCA ATAGGAGATG GCTGGCCACG TGCCAAATCG 
CCTAACGGCT CTAGCAGTGT TAACTGGCCA CCAGAATTTC GCCCTGGTGA ACCATGGAAA 
GGTTATCCAA ACATTGACCC TGAAACTGAC CCTTACGTCA CTCCTGGCAG TGTCATAAAC 
AATCTTTCAA TTAATACTGT GCGGGAAGTT GACCACCTCA GGGACAGGAA CAGTGGGTCA 
TCCTCATCCT TGAACACCAC GCTGCCTTCA ACTAGTGCCT GGTCATCCAT TCGTGCCTCC 
AACTACAACG TTCCCCTCAG CAGTACAGCA CAAAGCACTT CAGGTTCCAG TTGGGGTGAG 
AGCAGCTCAG GGAGAATAAC AAATTGGCTT GTTCTAAAGA ACCTCACACC TCAGATTGAC 
GGCTCAACTC TGCGTACTCT GTGCATGCAG CATGGCCCAC TGATAACATT CCACCTGAAC 
CTCCCACATG GAAATGCTTT GGTCCGTTAC AGTTCAAAAG AAGAGGTAGT GAAGGCACAA 
AAGTCTCTGC ACATGTGTGT ATTGGGGAAC ACTACTATTC TTGCTGAGTT TGCCAGTGAA 
GAGGAGATCA GCCGTTTCTT TGCACAAAGT CAGTCTCTGA CCCCTTCTCC CGGCTGGCAG 
TCTCTCGGGT CCAGCCAGAG CCGGCTGGGC TCCCTCGACT GTTCCCACTC GTTCTCCAGC 
CGGACCGATC TCAATCACTG GAATGGTGCT GGGCTGTCGG GAACTAACTG TGGAGACCTT 
CACGGCACTT CACTCTGGGG GACCCCACAT TATTCCACAA GCCTGTGGGG TCCTCCCAGC 
AGCAGCGACC CCCGGGGAAT TAGCAGCCCA TCCCCCATTA ATGCTTTTCT TTCTGTTGAC 
CACCTGGGTG GGGGTGGAGA GTCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005621461.1
CDS	278..6007
Translation	MQLVAEPGLEARCPGSQDIPLPEEWNRELEAKATKDVERTLSRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNPQQQQQPQPPQQPQQPPQQPPQPPQQQPQPAQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLSSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSPSDSSTNCKNAVVNDSPETEAWPSVPGSDPELASECMDADSASSSESERNITIMASGSTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSPGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLDNNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVGGSSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVMNGTSLSHLSNGEPKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGINGPMGTNFQVNTNKGGGVWESGATNSQNASWGSGNGANSGGSRRGWGTPAQNTGTNIPSVEWSKLPSNQHSNDSANGNGKKFTNGWKSTEEEDQASAASQTTEQNSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACTDKTSPTSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDAAATGMVKSNQWGNSKEEKSAWNDSQKSKQGWGDGQKSNQGWSVSASDNWGETSRSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVTSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPTKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSASAISNKEAGSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASNTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGTMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERSPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPSLLVKQQTPPSQQQPLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_005621404.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005621404.3

ORF Insert Sequence:

ATGCAACTAG TGGCAGAGCC AGGCCTAGAA GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG AGAATTGGAA GCTAAAGCTA CCAAAGACGT AGAAAGGACT 
CTTAGCAGGG ATTTAGTGCA AGAAGAAGAA CAGTTGATGG AAGAAAAGAA AAAGAAAAAA 
GACGACAAGA AAAAGAAGGA AGCTGCTCAA AAGAAGGCCA CTGAACAAAA AATCAAAGTG 
CCAGAACAGA TAAAGCCCAG TGTAAGCCAG CCTCAGCCTG CCAACTCTAA TAACGGCACT 
TCCACAGCAA CCAGCACTAA TAATAATGCC AAGCGAGCCA CAGCCAACAA TCCGCAGCAG 
CAGCAGCAGC CACAGCCGCC ACAGCAGCCA CAGCAGCCGC CGCAGCAGCC ACCGCAGCCG 
CCACAGCAGC AGCCGCAGCC AGCACAGGCC TTGCCTCGGT ATCCTCGGGA AGTACCACCA 
CGATTTCGCC ACCAGGAACA CAAACAGCTT CTAAAGAGGG GTCAGCATTT TCCTGTCATA 
GCAGCAAACC TGGGATCTGC TGTTAAAGTG TTAAGCAGCC AGTCAGAAAG CAGTGCTTTA 
ACAAATCAAC AGCCACAAAA TAACGGAGAG GTGCAGAACA GCAAAAACCA GTCAGATATA 
AACCACAGTA CTTCAGGATC CCATTATGAA AATTCACAGC GGGGACCTGT GTCTTCTCCG 
AGTGACTCTA GCACAAATTG TAAGAATGCT GTTGTAAACG ACTCCCCTGA GACAGAAGCA 
TGGCCCTCAG TCCCTGGCAG TGATCCAGAG TTGGCTTCAG AATGTATGGA TGCTGATTCT 
GCCTCCAGTT CTGAATCAGA GAGAAACATC ACTATCATGG CTTCAGGGAG CACAGGTGGT 
GAAAAAGATG GCCTTCGGAA TAGCACTGGA CTTGGTTCCC AAAACAAGTT TGTGGTTGGT 
AGCAGCAGCA ATAATGTGGG CCATGGAAGT AGTCCTGGGC CATGGGGTTT TTCCCATGGA 
GCCATAATAA GCACATGTCA GGTCTCTGTG GATGCTCCTG AAAGCAAATC AGAAAGTAGC 
AACAATAGAA TGAATGCTTG GGGCACTGTA AGTTCTTCAT CAAATGGAGG GTTAAATCCA 
AGCACTTTGA ATTCAGCTAG CAACCATGGT GCCTGGCCAG TATTAGACAA CAACGGACTT 
GCCCTCAAAG GGCCTGTAGG GAGCGGTAGT TCTGGCATCA ATATTCAGTG TAGTACCATA 
GGCCAGATGC CTAACAATCA GAGTATTAAC TCTAAAGTGG GTGGTTCTTC CACCCATGGT 
ACCTGGGGAA GCCTTCAGGA AACTTGTGAA TCTGAAGTAA GTGGTACACA GAAGGTTTCA 
TTCAGTGGTC AACCTCAGAA TATTACCACT GAGATGACTG GACCAAATAA CACTACTAAC 
TTTATGACCT CTAGTTTACC AAACTCCGGT TCAGTACAGA ATAACGAACT GCCTAGTAAT 
ACAGGGGCCT GGCGTGTGAG CACAATGAAT CATCCTCAGA TACAGGCTCC ATCGGTTATG 
AATGGCACTT CCCTTTCTCA TCTTAGCAAT GGAGAGCCAA AAAGTGGAGG CTCTTACGGT 
ACTACGTGGG GTGCCTATGG TTCTAATTAC TCTGGAGACA AATGTTCGAG CCCTAACGGC 
CAAGCTAATG GTGACACTGT GAATGCAACT CTAATGCAGC CTGGCATAAA TGGGCCTATG 
GGCACTAACT TTCAAGTGAA CACAAATAAA GGAGGAGGTG TGTGGGAGTC TGGGGCAACG 
AATTCCCAGA ATGCATCGTG GGGAAGTGGA AATGGCGCAA ATTCTGGAGG AAGTCGACGA 
GGATGGGGAA CTCCTGCACA AAACACTGGC ACTAATATAC CCAGCGTGGA GTGGAGCAAA 
CTGCCTAGCA ATCAGCATTC CAATGACAGT GCAAATGGCA ATGGTAAGAA GTTTACAAAT 
GGATGGAAAT CTACTGAGGA AGAGGATCAG GCTTCTGCCG CATCTCAGAC AACTGAGCAG 
AACAGTGTGT GGGCCAAAAC AGGAGGTACA GTGGAGAGTG AAGGTAGTAC AGAAAGCACT 
GGACGCCTCG AGGAGAAAGC CACTGGGGAA AATCAGAGTA GAGATCGGAG AAAAATTGAT 
CAGCACACAT TACTCCAAAG CATCGTAAAC AGAACTGACT TGGATCCACG TGTCCTCTCC 
AACTCTGGTT GGGGACAGAC TCCTATTAAG CAGAATACTG CCTGGGATAC GGAAACATCA 
CCTAGAGGGG AAAGAAAGAC TGACAATGGG ACAGAGGCCT GGGGAAGCTC TGCAACACAG 
ACTTTTAACT CAGGGGCATG TACAGATAAG ACTAGCCCTA CTAGTAATGA TACCTCATCT 
GTATCGGGGT GGGGAGATCC CAAACCTGCT CTGAGGTGGG GAGATTCCAA AGGCTCAAGC 
TGCCAGGGGG GATGGGAAGA TGATGCTGCT GCTACAGGAA TGGTCAAGAG CAATCAGTGG 
GGGAACAGCA AAGAAGAGAA GTCCGCATGG AATGATTCGC AAAAGAGCAA ACAGGGATGG 
GGTGATGGAC AGAAATCAAA CCAAGGGTGG TCCGTTTCTG CCAGTGATAA CTGGGGAGAA 
ACGTCAAGGA GTAACCATTG GGGTGAGGCT AATAAGAAAT CTAGCTCAGG AGGTAGTGAC 
AGTGACAGGT CTGTTTCTGG TTGGAATGAA CTTGGTAAAA CTAGTTCTTT TACTTGGGGA 
AATAACATAA ATCCAAATAA TTCGTCAGGA TGGGATGAAT CTTCTAAACC TAATCCTTCC 
CAGGGATGGG GAGATCCTCC AAAGTCTAAT CAGTCTCTAG GTTGGGGAGA TTCGTCAAAG 
CCAGTCACTT CTCCAGACTG GAACAAGCAA CAAGACATTG TCGGGTCTTG GGGAATCCCA 
CCAGCAACAG GCAAACCTCC TGGTACAGGC TGGCTGGGAG GACCTATACC AGCCCCAACA 
AAAGAAGAAG AGCCCACAGG CTGGGAGGAA CCATCCCCAG AATCCATACG TCGCAAAATG 
GAGATCGATG ACGGAACTTC AGCTTGGGGA GATCCGAGCA AATACAACTA CAAAAATGTG 
AACATGTGGA ACAAAAATGT CCCAAATGGC AGCAGCCGTT CAGACCAGCA AGCACAGGTA 
CACCAGTTGC TACCATCTGC AAGTGCCATC TCAAACAAAG AGGCAGGCAG TGGCTCTGGC 
TGGGGTGAGC CCTGGGGGGA GCCTTCTACT CCAGCCACAA CTGTGGATAA TGGTACTTCA 
GCATGGGGTA AACCCATAGA CAGTGGTCCC AGCTGGGGGG AGCCCATTGC TGCAGCATCC 
AACACATCCA CGTGGGGCTC CAGCTCTGTT GGTCCTCAAG CATTAAGCAA ATCTGGGCCA 
AAATCTATGC AAGATGGCTG GTGTGGTGAT GATATGCCAT TGCCTGGAAA TCGCCCCACT 
GGCTGGGAAG AGGAAGAGGA TGTAGAGATT GGAATGTGGA ATAGTAATTC ATCTCAAGAG 
CTTAACTCAT CTTTAAATTG GCCTCCATAT ACAAAGAAAA TGTCATCGAA GGGTCTGAGT 
GGCAAAAAAA GGAGAAGGGA AAGGGGAACC ATGAAAGGTG GAAACAAACA AGAAGAAGCA 
TGGATAAATC CATTCGTTAA ACAGTTTTCA AATATCAGTT TTTCGAGAGA CTCACCCGAA 
GAAAATGTAC AGAGCAATAA GATGGATCTT TCCGGAGGAA TGTTACAGGA CAAACGAATG 
GAGATAGATA AACATAGCCT AAATATTGGT GATTACAATC GAACGGTCGG GAAAGGCCCT 
GGTTCTCGGC CTCAGATTTC CAAAGAGTCT TCCATGGAGC GCAGCCCTTA CTTTGATAAG 
GATGGCATTG TAGCAGATGA ATCCCAAAAC ATGCAGTTTA TGTCCAGTCA AAGCATGAAG 
CTTCCCCCTT CAAATAGTGC ACTACCTAAC CAGGCCCTTG GCTCCATAGC AGGGCTGGGT 
ATGCAAAACT TGAATTCTGT TAGACAGAAT GGCAATCCCA GTATGTTTGG TGTTGGAAAC 
ACAGCAGCAC AACCCCGGGG CATGCAGCAG CCTCCAGCAC AACCTCTTAG TTCATCTCAG 
CCTAATCTTC GTGCTCAAGT GCCTCCTCCA TTACTCTCCC CTCAGGTTCC AGTTTCATTG 
CTGAAGTATG CACCAAACAA CGGTGGCCTG AATCCGCTCT TTGGCCCTCA ACAGGTAGCC 
ATGCTGAACC AGCTATCCCA ACTAAACCAG CTTTCTCAGA TCTCCCAGTT ACAGAGATTG 
TTAGCGCAGC AGCAAAGGGC GCAGAGTCAG AGAAGCGTGC CTTCTGGGAA CCGGCAGCAG 
CAAGACCAGC AGGGTCGACC TCTTAGCGTG CAGCAGCAGA TGATGCAGCA GTCCCGGCAA 
CTCGATCCCA GCCTGTTGGT GAAGCAGCAG ACCCCGCCAT CTCAGCAGCA GCCACTCCAT 
CAGCCAGCCA TGAAATCCTT CCTCGAGAAT GTCATGCCCC ACACTACACC TGAGCTGCAG 
AAAGGGCCAT CACCAATAAA TGCTTTCAGC AACTTCCCGA TAGGCTTGAA CTCAAACTTG 
AATGTAAATA TGGATATGAA CAGTATTAAA GAGCCACAGT CAAGACTAAG GAAGTGGACT 
ACAGTGGACA GCATTTCTGT GAACACATCT TTGGATCAAA ACTCCAGCAA ACATGGTGCT 
ATTTCAAGTG GTTTCAGGCT GGAAGAGTCT CCATTTGTTC CCTATGACTT TATGAACAGC 
AGTACTTCTC CAGCCAGCCC TCCGGGTTCA ATAGGAGATG GCTGGCCACG TGCCAAATCG 
CCTAACGGCT CTAGCAGTGT TAACTGGCCA CCAGAATTTC GCCCTGGTGA ACCATGGAAA 
GGTTATCCAA ACATTGACCC TGAAACTGAC CCTTACGTCA CTCCTGGCAG TGTCATAAAC 
AATCTTTCAA TTAATACTGT GCGGGAAGTT GACCACCTCA GGGACAGGAA CAGTGGGTCA 
TCCTCATCCT TGAACACCAC GCTGCCTTCA ACTAGTGCCT GGTCATCCAT TCGTGCCTCC 
AACTACAACG TTCCCCTCAG CAGTACAGCA CAAAGCACTT CAGGTTCCAG TTGGGGTGAG 
AGCAGCTCAG GGAGAATAAC AAATTGGCTT GTTCTAAAGA ACCTCACACC TCAGATTGAC 
GGCTCAACTC TGCGTACTCT GTGCATGCAG CATGGCCCAC TGATAACATT CCACCTGAAC 
CTCCCACATG GAAATGCTTT GGTCCGTTAC AGTTCAAAAG AAGAGGTAGT GAAGGCACAA 
AAGTCTCTGC ACATGTGTGT ATTGGGGAAC ACTACTATTC TTGCTGAGTT TGCCAGTGAA 
GAGGAGATCA GCCGTTTCTT TGCACAAAGT CAGTCTCTGA CCCCTTCTCC CGGCTGGCAG 
TCTCTCGGGT CCAGCCAGAG CCGGCTGGGC TCCCTCGACT GTTCCCACTC GTTCTCCAGC 
CGGACCGATC TCAATCACTG GAATGGTGCT GGGCTGTCGG GAACTAACTG TGGAGACCTT 
CACGGCACTT CACTCTGGGG GACCCCACAT TATTCCACAA GCCTGTGGGG TCCTCCCAGC 
AGCAGCGACC CCCGGGGAAT TAGCAGCCCA TCCCCCATTA ATGCTTTTCT TTCTGTTGAC 
CACCTGGGTG GGGGTGGAGA GTCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_022419940.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022275648.1 Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X12, mRNA.

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CloneID	OCe07962
Clone ID Related Accession (Same CDS sequence)	XM_022419940.1
Accession Version	XM_022419940.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	trinucleotide repeat-containing gene 6A protein isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAATGT ATAGCGATAT AAACCACAGT ACTTCAGGAT CCCATTATGA AAATTCACAG 
CGGGGACCTG TGTCTTCTCC GAGTGACTCT AGCACAAATT GTAAGAATGC TGTTGTAAAC 
GACTCCCCTG AGACAGAAGC ATGGCCCTCA GTCCCTGGCA GTGATCCAGA GTTGGCTTCA 
GAATGTATGG ATGCTGATTC TGCCTCCAGT TCTGAATCAG AGAGAAACAT CACTATCATG 
GCTTCAGGGA GCACAGGTGG TGAAAAAGAT GGCCTTCGGA ATAGCACTGG ACTTGGTTCC 
CAAAACAAGT TTGTGGTTGG TAGCAGCAGC AATAATGTGG GCCATGGAAG TAGTCCTGGG 
CCATGGGGTT TTTCCCATGG AGCCATAATA AGCACATGTC AGGTCTCTGT GGATGCTCCT 
GAAAGCAAAT CAGAAAGTAG CAACAATAGA ATGAATGCTT GGGGCACTGT AAGTTCTTCA 
TCAAATGGAG GGTTAAATCC AAGCACTTTG AATTCAGCTA GCAACCATGG TGCCTGGCCA 
GTATTAGACA ACAACGGACT TGCCCTCAAA GGGCCTGTAG GGAGCGGTAG TTCTGGCATC 
AATATTCAGT GTAGTACCAT AGGCCAGATG CCTAACAATC AGAGTATTAA CTCTAAAGTG 
GGTGGTTCTT CCACCCATGG TACCTGGGGA AGCCTTCAGG AAACTTGTGA ATCTGAAGTA 
AGTGGTACAC AGAAGGTTTC ATTCAGTGGT CAACCTCAGA ATATTACCAC TGAGATGACT 
GGACCAAATA ACACTACTAA CTTTATGACC TCTAGTTTAC CAAACTCCGG TTCAGTACAG 
AATAACGAAC TGCCTAGTAA TACAGGGGCC TGGCGTGTGA GCACAATGAA TCATCCTCAG 
ATACAGGCTC CATCGGTTAT GAATGGCACT TCCCTTTCTC ATCTTAGCAA TGGAGAGCCA 
AAAAGTGGAG GCTCTTACGG TACTACGTGG GGTGCCTATG GTTCTAATTA CTCTGGAGAC 
AAATGTTCGA GCCCTAACGG CCAAGCTAAT GGTGACACTG TGAATGCAAC TCTAATGCAG 
CCTGGCATAA ATGGGCCTAT GGGCACTAAC TTTCAAGTGA ACACAAATAA AGGAGGAGGT 
GTGTGGGAGT CTGGGGCAAC GAATTCCCAG AATGCATCGT GGGGAAGTGG AAATGGCGCA 
AATTCTGGAG GAAGTCGACG AGGATGGGGA ACTCCTGCAC AAAACACTGG CACTAATATA 
CCCAGCGTGG AGTGGAGCAA ACTGCCTAGC AATCAGCATT CCAATGACAG TGCAAATGGC 
AATGGTAAGA AGTTTACAAA TGGATGGAAA TCTACTGAGG AAGAGGATCA GGCTTCTGCC 
GCATCTCAGA CAACTGAGCA GAACAGTGTG TGGGCCAAAA CAGGAGGTAC AGTGGAGAGT 
GAAGGTAGTA CAGAAAGCAC TGGACGCCTC GAGGAGAAAG CCACTGGGGA AAATCAGAGT 
AGAGATCGGA GAAAAATTGA TCAGCACACA TTACTCCAAA GCATCGTAAA CAGAACTGAC 
TTGGATCCAC GTGTCCTCTC CAACTCTGGT TGGGGACAGA CTCCTATTAA GCAGAATACT 
GCCTGGGATA CGGAAACATC ACCTAGAGGG GAAAGAAAGA CTGACAATGG GACAGAGGCC 
TGGGGAAGCT CTGCAACACA GACTTTTAAC TCAGGGGCAT GTACAGATAA GACTAGCCCT 
ACTAGTAATG ATACCTCATC TGTATCGGGG TGGGGAGATC CCAAACCTGC TCTGAGGTGG 
GGAGATTCCA AAGGCTCAAG CTGCCAGGGG GGATGGGAAG ATGATGCTGC TGCTACAGGA 
ATGGTCAAGA GCAATCAGTG GGGGAACAGC AAAGAAGAGA AGTCCGCATG GAATGATTCG 
CAAAAGAGCA AACAGGGATG GGGTGATGGA CAGAAATCAA ACCAAGGGTG GTCCGTTTCT 
GCCAGTGATA ACTGGGGAGA AACGTCAAGG AGTAACCATT GGGGTGAGGC TAATAAGAAA 
TCTAGCTCAG GAGGTAGTGA CAGTGACAGG TCTGTTTCTG GTTGGAATGA ACTTGGTAAA 
ACTAGTTCTT TTACTTGGGG AAATAACATA AATCCAAATA ATTCGTCAGG ATGGGATGAA 
TCTTCTAAAC CTAATCCTTC CCAGGGATGG GGAGATCCTC CAAAGTCTAA TCAGTCTCTA 
GGTTGGGGAG ATTCGTCAAA GCCAGTCACT TCTCCAGACT GGAACAAGCA ACAAGACATT 
GTCGGGTCTT GGGGAATCCC ACCAGCAACA GGCAAACCTC CTGGTACAGG CTGGCTGGGA 
GGACCTATAC CAGCCCCAAC AAAAGAAGAA GAGCCCACAG GCTGGGAGGA ACCATCCCCA 
GAATCCATAC GTCGCAAAAT GGAGATCGAT GACGGAACTT CAGCTTGGGG AGATCCGAGC 
AAATACAACT ACAAAAATGT GAACATGTGG AACAAAAATG TCCCAAATGG CAGCAGCCGT 
TCAGACCAGC AAGCACAGGT ACACCAGTTG CTACCATCTG CAAGTGCCAT CTCAAACAAA 
GAGGCAGGCA GTGGCTCTGG CTGGGGTGAG CCCTGGGGGG AGCCTTCTAC TCCAGCCACA 
ACTGTGGATA ATGGTACTTC AGCATGGGGT AAACCCATAG ACAGTGGTCC CAGCTGGGGG 
GAGCCCATTG CTGCAGCATC CAACACATCC ACGTGGGGCT CCAGCTCTGT TGGTCCTCAA 
GCATTAAGCA AATCTGGGCC AAAATCTATG CAAGATGGCT GGTGTGGTGA TGATATGCCA 
TTGCCTGGAA ATCGCCCCAC TGGCTGGGAA GAGGAAGAGG ATGTAGAGAT TGGAATGTGG 
AATAGTAATT CATCTCAAGA GCTTAACTCA TCTTTAAATT GGCCTCCATA TACAAAGAAA 
ATGTCATCGA AGGGTCTGAG TGGCAAAAAA AGGAGAAGGG AAAGGGGAAC CATGAAAGGT 
GGAAACAAAC AAGAAGAAGC ATGGATAAAT CCATTCGTTA AACAGTTTTC AAATATCAGT 
TTTTCGAGAG ACTCACCCGA AGAAAATGTA CAGAGCAATA AGATGGATCT TTCCGGAGGA 
ATGTTACAGG ACAAACGAAT GGAGATAGAT AAACATAGCC TAAATATTGG TGATTACAAT 
CGAACGGTCG GGAAAGGCCC TGGTTCTCGG CCTCAGATTT CCAAAGAGTC TTCCATGGAG 
CGCAGCCCTT ACTTTGATAA GGATGGCATT GTAGCAGATG AATCCCAAAA CATGCAGTTT 
ATGTCCAGTC AAAGCATGAA GCTTCCCCCT TCAAATAGTG CACTACCTAA CCAGGCCCTT 
GGCTCCATAG CAGGGCTGGG TATGCAAAAC TTGAATTCTG TTAGACAGAA TGGCAATCCC 
AGTATGTTTG GTGTTGGAAA CACAGCAGCA CAACCCCGGG GCATGCAGCA GCCTCCAGCA 
CAACCTCTTA GTTCATCTCA GCCTAATCTT CGTGCTCAAG TGCCTCCTCC ATTACTCTCC 
CCTCAGGTTC CAGTTTCATT GCTGAAGTAT GCACCAAACA ACGGTGGCCT GAATCCGCTC 
TTTGGCCCTC AACAGGTAGC CATGCTGAAC CAGCTATCCC AACTAAACCA GCTTTCTCAG 
ATCTCCCAGT TACAGAGATT GTTAGCGCAG CAGCAAAGGG CGCAGAGTCA GAGAAGCGTG 
CCTTCTGGGA ACCGGCAGCA GCAAGACCAG CAGGGTCGAC CTCTTAGCGT GCAGCAGCAG 
ATGATGCAGC AGTCCCGGCA ACTCGATCCC AGCCTGTTGG TGAAGCAGCA GACCCCGCCA 
TCTCAGCAGC AGCCACTCCA TCAGCCAGCC ATGAAATCCT TCCTCGAGAA TGTCATGCCC 
CACACTACAC CTGAGCTGCA GAAAGGGCCA TCACCAATAA ATGCTTTCAG CAACTTCCCG 
ATAGGCTTGA ACTCAAACTT GAATGTAAAT ATGGATATGA ACAGTATTAA AGAGCCACAG 
TCAAGACTAA GGAAGTGGAC TACAGTGGAC AGCATTTCTG TGAACACATC TTTGGATCAA 
AACTCCAGCA AACATGGTGC TATTTCAAGT GGTTTCAGGC TGGAAGAGTC TCCATTTGTT 
CCCTATGACT TTATGAACAG CAGTACTTCT CCAGCCAGCC CTCCGGGTTC AATAGGAGAT 
GGCTGGCCAC GTGCCAAATC GCCTAACGGC TCTAGCAGTG TTAACTGGCC ACCAGAATTT 
CGCCCTGGTG AACCATGGAA AGGTTATCCA AACATTGACC CTGAAACTGA CCCTTACGTC 
ACTCCTGGCA GTGTCATAAA CAATCTTTCA ATTAATACTG TGCGGGAAGT TGACCACCTC 
AGGGACAGGA ACAGTGGGTC ATCCTCATCC TTGAACACCA CGCTGCCTTC AACTAGTGCC 
TGGTCATCCA TTCGTGCCTC CAACTACAAC GTTCCCCTCA GCAGTACAGC ACAAAGCACT 
TCAGCCAGAA ATAGTGATTC CAAATTGACA TGGTCTCCTG GTTCAGTTAC AAACACCTCT 
CTGGCTCATG AGCTGTGGAA GGTCCCTTTA CCACCTAAAA ACATCACTGC TCCGTCCCGC 
CCACCTCCGG GACTCACTGG TCAGAAGCCA CCCTTGTCTA CGTGGGATAA CTCTCCCCTT 
CGTGTAGGTG GAGGATGGGG AAATTCTGAC GCCAGGTACA CCCCAGGTTC CAGTTGGGGT 
GAGAGCAGCT CAGGGAGAAT AACAAATTGG CTTGTTCTAA AGAACCTCAC ACCTCAGATT 
GACGGCTCAA CTCTGCGTAC TCTGTGCATG CAGCATGGCC CACTGATAAC ATTCCACCTG 
AACCTCCCAC ATGGAAATGC TTTGGTCCGT TACAGTTCAA AAGAAGAGGT AGTGAAGGCA 
CAAAAGTCTC TGCACATGTG TGTATTGGGG AACACTACTA TTCTTGCTGA GTTTGCCAGT 
GAAGAGGAGA TCAGCCGTTT CTTTGCACAA AGTCAGTCTC TGACCCCTTC TCCCGGCTGG 
CAGTCTCTCG GGTCCAGCCA GAGCCGGCTG GGCTCCCTCG ACTGTTCCCA CTCGTTCTCC 
AGCCGGACCG ATCTCAATCA CTGGAATGGT GCTGGGCTGT CGGGAACTAA CTGTGGAGAC 
CTTCACGGCA CTTCACTCTG GGGGACCCCA CATTATTCCA CAAGCCTGTG GGGTCCTCCC 
AGCAGCAGCG ACCCCCGGGG AATTAGCAGC CCATCCCCCA TTAATGCTTT TCTTTCTGTT 
GACCACCTGG GTGGGGGTGG AGAGTCCATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022275648.1
CDS	10..5322
Translation	MIMYSDINHSTSGSHYENSQRGPVSSPSDSSTNCKNAVVNDSPETEAWPSVPGSDPELASECMDADSASSSESERNITIMASGSTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSPGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLDNNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVGGSSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVMNGTSLSHLSNGEPKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGINGPMGTNFQVNTNKGGGVWESGATNSQNASWGSGNGANSGGSRRGWGTPAQNTGTNIPSVEWSKLPSNQHSNDSANGNGKKFTNGWKSTEEEDQASAASQTTEQNSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACTDKTSPTSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDAAATGMVKSNQWGNSKEEKSAWNDSQKSKQGWGDGQKSNQGWSVSASDNWGETSRSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVTSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPTKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSASAISNKEAGSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASNTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGTMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERSPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPSLLVKQQTPPSQQQPLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_022419940.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022419940.1

ORF Insert Sequence:

ATGATAATGT ATAGCGATAT AAACCACAGT ACTTCAGGAT CCCATTATGA AAATTCACAG 
CGGGGACCTG TGTCTTCTCC GAGTGACTCT AGCACAAATT GTAAGAATGC TGTTGTAAAC 
GACTCCCCTG AGACAGAAGC ATGGCCCTCA GTCCCTGGCA GTGATCCAGA GTTGGCTTCA 
GAATGTATGG ATGCTGATTC TGCCTCCAGT TCTGAATCAG AGAGAAACAT CACTATCATG 
GCTTCAGGGA GCACAGGTGG TGAAAAAGAT GGCCTTCGGA ATAGCACTGG ACTTGGTTCC 
CAAAACAAGT TTGTGGTTGG TAGCAGCAGC AATAATGTGG GCCATGGAAG TAGTCCTGGG 
CCATGGGGTT TTTCCCATGG AGCCATAATA AGCACATGTC AGGTCTCTGT GGATGCTCCT 
GAAAGCAAAT CAGAAAGTAG CAACAATAGA ATGAATGCTT GGGGCACTGT AAGTTCTTCA 
TCAAATGGAG GGTTAAATCC AAGCACTTTG AATTCAGCTA GCAACCATGG TGCCTGGCCA 
GTATTAGACA ACAACGGACT TGCCCTCAAA GGGCCTGTAG GGAGCGGTAG TTCTGGCATC 
AATATTCAGT GTAGTACCAT AGGCCAGATG CCTAACAATC AGAGTATTAA CTCTAAAGTG 
GGTGGTTCTT CCACCCATGG TACCTGGGGA AGCCTTCAGG AAACTTGTGA ATCTGAAGTA 
AGTGGTACAC AGAAGGTTTC ATTCAGTGGT CAACCTCAGA ATATTACCAC TGAGATGACT 
GGACCAAATA ACACTACTAA CTTTATGACC TCTAGTTTAC CAAACTCCGG TTCAGTACAG 
AATAACGAAC TGCCTAGTAA TACAGGGGCC TGGCGTGTGA GCACAATGAA TCATCCTCAG 
ATACAGGCTC CATCGGTTAT GAATGGCACT TCCCTTTCTC ATCTTAGCAA TGGAGAGCCA 
AAAAGTGGAG GCTCTTACGG TACTACGTGG GGTGCCTATG GTTCTAATTA CTCTGGAGAC 
AAATGTTCGA GCCCTAACGG CCAAGCTAAT GGTGACACTG TGAATGCAAC TCTAATGCAG 
CCTGGCATAA ATGGGCCTAT GGGCACTAAC TTTCAAGTGA ACACAAATAA AGGAGGAGGT 
GTGTGGGAGT CTGGGGCAAC GAATTCCCAG AATGCATCGT GGGGAAGTGG AAATGGCGCA 
AATTCTGGAG GAAGTCGACG AGGATGGGGA ACTCCTGCAC AAAACACTGG CACTAATATA 
CCCAGCGTGG AGTGGAGCAA ACTGCCTAGC AATCAGCATT CCAATGACAG TGCAAATGGC 
AATGGTAAGA AGTTTACAAA TGGATGGAAA TCTACTGAGG AAGAGGATCA GGCTTCTGCC 
GCATCTCAGA CAACTGAGCA GAACAGTGTG TGGGCCAAAA CAGGAGGTAC AGTGGAGAGT 
GAAGGTAGTA CAGAAAGCAC TGGACGCCTC GAGGAGAAAG CCACTGGGGA AAATCAGAGT 
AGAGATCGGA GAAAAATTGA TCAGCACACA TTACTCCAAA GCATCGTAAA CAGAACTGAC 
TTGGATCCAC GTGTCCTCTC CAACTCTGGT TGGGGACAGA CTCCTATTAA GCAGAATACT 
GCCTGGGATA CGGAAACATC ACCTAGAGGG GAAAGAAAGA CTGACAATGG GACAGAGGCC 
TGGGGAAGCT CTGCAACACA GACTTTTAAC TCAGGGGCAT GTACAGATAA GACTAGCCCT 
ACTAGTAATG ATACCTCATC TGTATCGGGG TGGGGAGATC CCAAACCTGC TCTGAGGTGG 
GGAGATTCCA AAGGCTCAAG CTGCCAGGGG GGATGGGAAG ATGATGCTGC TGCTACAGGA 
ATGGTCAAGA GCAATCAGTG GGGGAACAGC AAAGAAGAGA AGTCCGCATG GAATGATTCG 
CAAAAGAGCA AACAGGGATG GGGTGATGGA CAGAAATCAA ACCAAGGGTG GTCCGTTTCT 
GCCAGTGATA ACTGGGGAGA AACGTCAAGG AGTAACCATT GGGGTGAGGC TAATAAGAAA 
TCTAGCTCAG GAGGTAGTGA CAGTGACAGG TCTGTTTCTG GTTGGAATGA ACTTGGTAAA 
ACTAGTTCTT TTACTTGGGG AAATAACATA AATCCAAATA ATTCGTCAGG ATGGGATGAA 
TCTTCTAAAC CTAATCCTTC CCAGGGATGG GGAGATCCTC CAAAGTCTAA TCAGTCTCTA 
GGTTGGGGAG ATTCGTCAAA GCCAGTCACT TCTCCAGACT GGAACAAGCA ACAAGACATT 
GTCGGGTCTT GGGGAATCCC ACCAGCAACA GGCAAACCTC CTGGTACAGG CTGGCTGGGA 
GGACCTATAC CAGCCCCAAC AAAAGAAGAA GAGCCCACAG GCTGGGAGGA ACCATCCCCA 
GAATCCATAC GTCGCAAAAT GGAGATCGAT GACGGAACTT CAGCTTGGGG AGATCCGAGC 
AAATACAACT ACAAAAATGT GAACATGTGG AACAAAAATG TCCCAAATGG CAGCAGCCGT 
TCAGACCAGC AAGCACAGGT ACACCAGTTG CTACCATCTG CAAGTGCCAT CTCAAACAAA 
GAGGCAGGCA GTGGCTCTGG CTGGGGTGAG CCCTGGGGGG AGCCTTCTAC TCCAGCCACA 
ACTGTGGATA ATGGTACTTC AGCATGGGGT AAACCCATAG ACAGTGGTCC CAGCTGGGGG 
GAGCCCATTG CTGCAGCATC CAACACATCC ACGTGGGGCT CCAGCTCTGT TGGTCCTCAA 
GCATTAAGCA AATCTGGGCC AAAATCTATG CAAGATGGCT GGTGTGGTGA TGATATGCCA 
TTGCCTGGAA ATCGCCCCAC TGGCTGGGAA GAGGAAGAGG ATGTAGAGAT TGGAATGTGG 
AATAGTAATT CATCTCAAGA GCTTAACTCA TCTTTAAATT GGCCTCCATA TACAAAGAAA 
ATGTCATCGA AGGGTCTGAG TGGCAAAAAA AGGAGAAGGG AAAGGGGAAC CATGAAAGGT 
GGAAACAAAC AAGAAGAAGC ATGGATAAAT CCATTCGTTA AACAGTTTTC AAATATCAGT 
TTTTCGAGAG ACTCACCCGA AGAAAATGTA CAGAGCAATA AGATGGATCT TTCCGGAGGA 
ATGTTACAGG ACAAACGAAT GGAGATAGAT AAACATAGCC TAAATATTGG TGATTACAAT 
CGAACGGTCG GGAAAGGCCC TGGTTCTCGG CCTCAGATTT CCAAAGAGTC TTCCATGGAG 
CGCAGCCCTT ACTTTGATAA GGATGGCATT GTAGCAGATG AATCCCAAAA CATGCAGTTT 
ATGTCCAGTC AAAGCATGAA GCTTCCCCCT TCAAATAGTG CACTACCTAA CCAGGCCCTT 
GGCTCCATAG CAGGGCTGGG TATGCAAAAC TTGAATTCTG TTAGACAGAA TGGCAATCCC 
AGTATGTTTG GTGTTGGAAA CACAGCAGCA CAACCCCGGG GCATGCAGCA GCCTCCAGCA 
CAACCTCTTA GTTCATCTCA GCCTAATCTT CGTGCTCAAG TGCCTCCTCC ATTACTCTCC 
CCTCAGGTTC CAGTTTCATT GCTGAAGTAT GCACCAAACA ACGGTGGCCT GAATCCGCTC 
TTTGGCCCTC AACAGGTAGC CATGCTGAAC CAGCTATCCC AACTAAACCA GCTTTCTCAG 
ATCTCCCAGT TACAGAGATT GTTAGCGCAG CAGCAAAGGG CGCAGAGTCA GAGAAGCGTG 
CCTTCTGGGA ACCGGCAGCA GCAAGACCAG CAGGGTCGAC CTCTTAGCGT GCAGCAGCAG 
ATGATGCAGC AGTCCCGGCA ACTCGATCCC AGCCTGTTGG TGAAGCAGCA GACCCCGCCA 
TCTCAGCAGC AGCCACTCCA TCAGCCAGCC ATGAAATCCT TCCTCGAGAA TGTCATGCCC 
CACACTACAC CTGAGCTGCA GAAAGGGCCA TCACCAATAA ATGCTTTCAG CAACTTCCCG 
ATAGGCTTGA ACTCAAACTT GAATGTAAAT ATGGATATGA ACAGTATTAA AGAGCCACAG 
TCAAGACTAA GGAAGTGGAC TACAGTGGAC AGCATTTCTG TGAACACATC TTTGGATCAA 
AACTCCAGCA AACATGGTGC TATTTCAAGT GGTTTCAGGC TGGAAGAGTC TCCATTTGTT 
CCCTATGACT TTATGAACAG CAGTACTTCT CCAGCCAGCC CTCCGGGTTC AATAGGAGAT 
GGCTGGCCAC GTGCCAAATC GCCTAACGGC TCTAGCAGTG TTAACTGGCC ACCAGAATTT 
CGCCCTGGTG AACCATGGAA AGGTTATCCA AACATTGACC CTGAAACTGA CCCTTACGTC 
ACTCCTGGCA GTGTCATAAA CAATCTTTCA ATTAATACTG TGCGGGAAGT TGACCACCTC 
AGGGACAGGA ACAGTGGGTC ATCCTCATCC TTGAACACCA CGCTGCCTTC AACTAGTGCC 
TGGTCATCCA TTCGTGCCTC CAACTACAAC GTTCCCCTCA GCAGTACAGC ACAAAGCACT 
TCAGCCAGAA ATAGTGATTC CAAATTGACA TGGTCTCCTG GTTCAGTTAC AAACACCTCT 
CTGGCTCATG AGCTGTGGAA GGTCCCTTTA CCACCTAAAA ACATCACTGC TCCGTCCCGC 
CCACCTCCGG GACTCACTGG TCAGAAGCCA CCCTTGTCTA CGTGGGATAA CTCTCCCCTT 
CGTGTAGGTG GAGGATGGGG AAATTCTGAC GCCAGGTACA CCCCAGGTTC CAGTTGGGGT 
GAGAGCAGCT CAGGGAGAAT AACAAATTGG CTTGTTCTAA AGAACCTCAC ACCTCAGATT 
GACGGCTCAA CTCTGCGTAC TCTGTGCATG CAGCATGGCC CACTGATAAC ATTCCACCTG 
AACCTCCCAC ATGGAAATGC TTTGGTCCGT TACAGTTCAA AAGAAGAGGT AGTGAAGGCA 
CAAAAGTCTC TGCACATGTG TGTATTGGGG AACACTACTA TTCTTGCTGA GTTTGCCAGT 
GAAGAGGAGA TCAGCCGTTT CTTTGCACAA AGTCAGTCTC TGACCCCTTC TCCCGGCTGG 
CAGTCTCTCG GGTCCAGCCA GAGCCGGCTG GGCTCCCTCG ACTGTTCCCA CTCGTTCTCC 
AGCCGGACCG ATCTCAATCA CTGGAATGGT GCTGGGCTGT CGGGAACTAA CTGTGGAGAC 
CTTCACGGCA CTTCACTCTG GGGGACCCCA CATTATTCCA CAAGCCTGTG GGGTCCTCCC 
AGCAGCAGCG ACCCCCGGGG AATTAGCAGC CCATCCCCCA TTAATGCTTT TCTTTCTGTT 
GACCACCTGG GTGGGGGTGG AGAGTCCATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_022419937.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022275645.1 Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X7, mRNA.

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CloneID	OCe288916
Clone ID Related Accession (Same CDS sequence)	XM_022419937.1
Accession Version	XM_022419937.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	trinucleotide repeat-containing gene 6A protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACTAG TGGCAGAGCC AGGCCTAGAA GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG AGAATTGGAA GCTAAAGCTA CCAAAGACGT AGAAAGGACT 
CTTAGCAGGG ATTTAGTGCA AGAAGAAGAA CAGTTGATGG AAGAAAAGAA AAAGAAAAAA 
GACGACAAGA AAAAGAAGGA AGCTGCTCAA AAGAAGGCCA CTGAACAAAA AATCAAAGTG 
CCAGAACAGA TAAAGCCCAG TGTAAGCCAG CCTCAGCCTG CCAACTCTAA TAACGGCACT 
TCCACAGCAA CCAGCACTAA TAATAATGCC AAGCGAGCCA CAGCCAACAA TCCGCAGCAG 
CAGCAGCAGC CACAGCCGCC ACAGCAGCCA CAGCAGCCGC CGCAGCAGCC ACCGCAGCCG 
CCACAGCAGC AGCCGCAGCC AGCACAGGCC TTGCCTCGGT ATCCTCGGGA AGTACCACCA 
CGATTTCGCC ACCAGGAACA CAAACAGCTT CTAAAGAGGG GTCAGCATTT TCCTGTCATA 
GCAGCAAACC TGGGATCTGC TGTTAAAGTG TTAAGCAGCC AGTCAGAAAG CAGTGCTTTA 
ACAAATCAAC AGCCACAAAA TAACGGAGAG GTGCAGAACA GCAAAAACCA GTCAGATATA 
AACCACAGTA CTTCAGGATC CCATTATGAA AATTCACAGC GGGGACCTGT GTCTTCTCCG 
AGTGACTCTA GCACAAATTG TAAGAATGCT GTTGTAAACG ACTCCCCTGA GACAGAAGCA 
TGGCCCTCAG TCCCTGGCAG TGATCCAGAG TTGGCTTCAG AATGTATGGA TGCTGATTCT 
GCCTCCAGTT CTGAATCAGA GAGAAACATC ACTATCATGG CTTCAGGGAG CACAGGTGGT 
GAAAAAGATG GCCTTCGGAA TAGCACTGGA CTTGGTTCCC AAAACAAGTT TGTGGTTGGT 
AGCAGCAGCA ATAATGTGGG CCATGGAAGT AGTCCTGGGC CATGGGGTTT TTCCCATGGA 
GCCATAATAA GCACATGTCA GGTCTCTGTG GATGCTCCTG AAAGCAAATC AGAAAGTAGC 
AACAATAGAA TGAATGCTTG GGGCACTGTA AGTTCTTCAT CAAATGGAGG GTTAAATCCA 
AGCACTTTGA ATTCAGCTAG CAACCATGGT GCCTGGCCAG TATTAGACAA CAACGGACTT 
GCCCTCAAAG GGCCTGTAGG GAGCGGTAGT TCTGGCATCA ATATTCAGTG TAGTACCATA 
GGCCAGATGC CTAACAATCA GAGTATTAAC TCTAAAGTGG GTGGTTCTTC CACCCATGGT 
ACCTGGGGAA GCCTTCAGGA AACTTGTGAA TCTGAAGTAA GTGGTACACA GAAGGTTTCA 
TTCAGTGGTC AACCTCAGAA TATTACCACT GAGATGACTG GACCAAATAA CACTACTAAC 
TTTATGACCT CTAGTTTACC AAACTCCGGT TCAGTACAGA ATAACGAACT GCCTAGTAAT 
ACAGGGGCCT GGCGTGTGAG CACAATGAAT CATCCTCAGA TACAGGCTCC ATCGGTTATG 
AATGGCACTT CCCTTTCTCA TCTTAGCAAT GGAGAGCCAA AAAGTGGAGG CTCTTACGGT 
ACTACGTGGG GTGCCTATGG TTCTAATTAC TCTGGAGACA AATGTTCGAG CCCTAACGGC 
CAAGCTAATG GTGACACTGT GAATGCAACT CTAATGCAGC CTGGCATAAA TGGGCCTATG 
GGCACTAACT TTCAAGTGAA CACAAATAAA GGAGGAGGTG TGTGGGAGTC TGGGGCAACG 
AATTCCCAGA ATGCATCGTG GGGAAGTGGA AATGGCGCAA ATTCTGGAGG AAGTCGACGA 
GGATGGGGAA CTCCTGCACA AAACACTGGC ACTAATATAC CCAGCGTGGA GTGGAGCAAA 
CTGCCTAGCA ATCAGCATTC CAATGACAGT GCAAATGGCA ATGGTAAGAA GTTTACAAAT 
GGATGGAAAT CTACTGAGGA AGAGGATCAG GCTTCTGCCG CATCTCAGAC AACTGAGCAG 
AACAGTGTGT GGGCCAAAAC AGGAGGTACA GTGGAGAGTG AAGGTAGTAC AGAAAGCACT 
GGACGCCTCG AGGAGAAAGC CACTGGGGAA AATCAGAGTA GAGATCGGAG AAAAATTGAT 
CAGCACACAT TACTCCAAAG CATCGTAAAC AGAACTGACT TGGATCCACG TGTCCTCTCC 
AACTCTGGTT GGGGACAGAC TCCTATTAAG CAGAATACTG CCTGGGATAC GGAAACATCA 
CCTAGAGGGG AAAGAAAGAC TGACAATGGG ACAGAGGCCT GGGGAAGCTC TGCAACACAG 
ACTTTTAACT CAGGGGCATG TACAGATAAG ACTAGCCCTA CTAGTAATGA TACCTCATCT 
GTATCGGGGT GGGGAGATCC CAAACCTGCT CTGAGGTGGG GAGATTCCAA AGGCTCAAGC 
TGCCAGGGGG GATGGGAAGA TGATGCTGCT GCTACAGGAA TGGTCAAGAG CAATCAGTGG 
GGGAACAGCA AAGAAGAGAA GTCCGCATGG AATGATTCGC AAAAGAGCAA ACAGGGATGG 
GGTGATGGAC AGAAATCAAA CCAAGGGTGG TCCGTTTCTG CCAGTGATAA CTGGGGAGAA 
ACGTCAAGGA GTAACCATTG GGGTGAGGCT AATAAGAAAT CTAGCTCAGG AGGTAGTGAC 
AGTGACAGGT CTGTTTCTGG TTGGAATGAA CTTGGTAAAA CTAGTTCTTT TACTTGGGGA 
AATAACATAA ATCCAAATAA TTCGTCAGGA TGGGATGAAT CTTCTAAACC TAATCCTTCC 
CAGGGATGGG GAGATCCTCC AAAGTCTAAT CAGTCTCTAG GTTGGGGAGA TTCGTCAAAG 
CCAGTCACTT CTCCAGACTG GAACAAGCAA CAAGACATTG TCGGGTCTTG GGGAATCCCA 
CCAGCAACAG GCAAACCTCC TGGTACAGGC TGGCTGGGAG GACCTATACC AGCCCCAACA 
AAAGAAGAAG AGCCCACAGG CTGGGAGGAA CCATCCCCAG AATCCATACG TCGCAAAATG 
GAGATCGATG ACGGAACTTC AGCTTGGGGA GATCCGAGCA AATACAACTA CAAAAATGTG 
AACATGTGGA ACAAAAATGT CCCAAATGGC AGCAGCCGTT CAGACCAGCA AGCACAGGTA 
CACCAGTTGC TACCATCTGC AAGTGCCATC TCAAACAAAG AGGCAGGCAG TGGCTCTGGG 
CCAAAATCTA TGCAAGATGG CTGGTGTGGT GATGATATGC CATTGCCTGG AAATCGCCCC 
ACTGGCTGGG AAGAGGAAGA GGATGTAGAG ATTGGAATGT GGAATAGTAA TTCATCTCAA 
GAGCTTAACT CATCTTTAAA TTGGCCTCCA TATACAAAGA AAATGTCATC GAAGGGTCTG 
AGTGGCAAAA AAAGGAGAAG GGAAAGGGGA ACCATGAAAG GTGGAAACAA ACAAGAAGAA 
GCATGGATAA ATCCATTCGT TAAACAGTTT TCAAATATCA GTTTTTCGAG AGACTCACCC 
GAAGAAAATG TACAGAGCAA TAAGATGGAT CTTTCCGGAG GAATGTTACA GGACAAACGA 
ATGGAGATAG ATAAACATAG CCTAAATATT GGTGATTACA ATCGAACGGT CGGGAAAGGC 
CCTGGTTCTC GGCCTCAGAT TTCCAAAGAG TCTTCCATGG AGCGCAGCCC TTACTTTGAT 
AAGGATGGCA TTGTAGCAGA TGAATCCCAA AACATGCAGT TTATGTCCAG TCAAAGCATG 
AAGCTTCCCC CTTCAAATAG TGCACTACCT AACCAGGCCC TTGGCTCCAT AGCAGGGCTG 
GGTATGCAAA ACTTGAATTC TGTTAGACAG AATGGCAATC CCAGTATGTT TGGTGTTGGA 
AACACAGCAG CACAACCCCG GGGCATGCAG CAGCCTCCAG CACAACCTCT TAGTTCATCT 
CAGCCTAATC TTCGTGCTCA AGTGCCTCCT CCATTACTCT CCCCTCAGGT TCCAGTTTCA 
TTGCTGAAGT ATGCACCAAA CAACGGTGGC CTGAATCCGC TCTTTGGCCC TCAACAGGTA 
GCCATGCTGA ACCAGCTATC CCAACTAAAC CAGCTTTCTC AGATCTCCCA GTTACAGAGA 
TTGTTAGCGC AGCAGCAAAG GGCGCAGAGT CAGAGAAGCG TGCCTTCTGG GAACCGGCAG 
CAGCAAGACC AGCAGGGTCG ACCTCTTAGC GTGCAGCAGC AGATGATGCA GCAGTCCCGG 
CAACTCGATC CCAGCCTGTT GGTGAAGCAG CAGACCCCGC CATCTCAGCA GCAGCCACTC 
CATCAGCCAG CCATGAAATC CTTCCTCGAG AATGTCATGC CCCACACTAC ACCTGAGCTG 
CAGAAAGGGC CATCACCAAT AAATGCTTTC AGCAACTTCC CGATAGGCTT GAACTCAAAC 
TTGAATGTAA ATATGGATAT GAACAGTATT AAAGAGCCAC AGTCAAGACT AAGGAAGTGG 
ACTACAGTGG ACAGCATTTC TGTGAACACA TCTTTGGATC AAAACTCCAG CAAACATGGT 
GCTATTTCAA GTGGTTTCAG GCTGGAAGAG TCTCCATTTG TTCCCTATGA CTTTATGAAC 
AGCAGTACTT CTCCAGCCAG CCCTCCGGGT TCAATAGGAG ATGGCTGGCC ACGTGCCAAA 
TCGCCTAACG GCTCTAGCAG TGTTAACTGG CCACCAGAAT TTCGCCCTGG TGAACCATGG 
AAAGGTTATC CAAACATTGA CCCTGAAACT GACCCTTACG TCACTCCTGG CAGTGTCATA 
AACAATCTTT CAATTAATAC TGTGCGGGAA GTTGACCACC TCAGGGACAG GAACAGTGGG 
TCATCCTCAT CCTTGAACAC CACGCTGCCT TCAACTAGTG CCTGGTCATC CATTCGTGCC 
TCCAACTACA ACGTTCCCCT CAGCAGTACA GCACAAAGCA CTTCAGCCAG AAATAGTGAT 
TCCAAATTGA CATGGTCTCC TGGTTCAGTT ACAAACACCT CTCTGGCTCA TGAGCTGTGG 
AAGGTCCCTT TACCACCTAA AAACATCACT GCTCCGTCCC GCCCACCTCC GGGACTCACT 
GGTCAGAAGC CACCCTTGTC TACGTGGGAT AACTCTCCCC TTCGTGTAGG TGGAGGATGG 
GGAAATTCTG ACGCCAGGTA CACCCCAGGT TCCAGTTGGG GTGAGAGCAG CTCAGGGAGA 
ATAACAAATT GGCTTGTTCT AAAGAACCTC ACACCTCAGA TTGACGGCTC AACTCTGCGT 
ACTCTGTGCA TGCAGCATGG CCCACTGATA ACATTCCACC TGAACCTCCC ACATGGAAAT 
GCTTTGGTCC GTTACAGTTC AAAAGAAGAG GTAGTGAAGG CACAAAAGTC TCTGCACATG 
TGTGTATTGG GGAACACTAC TATTCTTGCT GAGTTTGCCA GTGAAGAGGA GATCAGCCGT 
TTCTTTGCAC AAAGTCAGTC TCTGACCCCT TCTCCCGGCT GGCAGTCTCT CGGGTCCAGC 
CAGAGCCGGC TGGGCTCCCT CGACTGTTCC CACTCGTTCT CCAGCCGGAC CGATCTCAAT 
CACTGGAATG GTGCTGGGCT GTCGGGAACT AACTGTGGAG ACCTTCACGG CACTTCACTC 
TGGGGGACCC CACATTATTC CACAAGCCTG TGGGGTCCTC CCAGCAGCAG CGACCCCCGG 
GGAATTAGCA GCCCATCCCC CATTAATGCT TTTCTTTCTG TTGACCACCT GGGTGGGGGT 
GGAGAGTCCA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022275645.1
CDS	277..6051
Translation	MQLVAEPGLEARCPGSQDIPLPEEWNRELEAKATKDVERTLSRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNPQQQQQPQPPQQPQQPPQQPPQPPQQQPQPAQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLSSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSPSDSSTNCKNAVVNDSPETEAWPSVPGSDPELASECMDADSASSSESERNITIMASGSTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSPGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLDNNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVGGSSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVMNGTSLSHLSNGEPKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGINGPMGTNFQVNTNKGGGVWESGATNSQNASWGSGNGANSGGSRRGWGTPAQNTGTNIPSVEWSKLPSNQHSNDSANGNGKKFTNGWKSTEEEDQASAASQTTEQNSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACTDKTSPTSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDAAATGMVKSNQWGNSKEEKSAWNDSQKSKQGWGDGQKSNQGWSVSASDNWGETSRSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVTSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPTKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSASAISNKEAGSGSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGTMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERSPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPSLLVKQQTPPSQQQPLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_022419937.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022419937.1

ORF Insert Sequence:

ATGCAACTAG TGGCAGAGCC AGGCCTAGAA GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG AGAATTGGAA GCTAAAGCTA CCAAAGACGT AGAAAGGACT 
CTTAGCAGGG ATTTAGTGCA AGAAGAAGAA CAGTTGATGG AAGAAAAGAA AAAGAAAAAA 
GACGACAAGA AAAAGAAGGA AGCTGCTCAA AAGAAGGCCA CTGAACAAAA AATCAAAGTG 
CCAGAACAGA TAAAGCCCAG TGTAAGCCAG CCTCAGCCTG CCAACTCTAA TAACGGCACT 
TCCACAGCAA CCAGCACTAA TAATAATGCC AAGCGAGCCA CAGCCAACAA TCCGCAGCAG 
CAGCAGCAGC CACAGCCGCC ACAGCAGCCA CAGCAGCCGC CGCAGCAGCC ACCGCAGCCG 
CCACAGCAGC AGCCGCAGCC AGCACAGGCC TTGCCTCGGT ATCCTCGGGA AGTACCACCA 
CGATTTCGCC ACCAGGAACA CAAACAGCTT CTAAAGAGGG GTCAGCATTT TCCTGTCATA 
GCAGCAAACC TGGGATCTGC TGTTAAAGTG TTAAGCAGCC AGTCAGAAAG CAGTGCTTTA 
ACAAATCAAC AGCCACAAAA TAACGGAGAG GTGCAGAACA GCAAAAACCA GTCAGATATA 
AACCACAGTA CTTCAGGATC CCATTATGAA AATTCACAGC GGGGACCTGT GTCTTCTCCG 
AGTGACTCTA GCACAAATTG TAAGAATGCT GTTGTAAACG ACTCCCCTGA GACAGAAGCA 
TGGCCCTCAG TCCCTGGCAG TGATCCAGAG TTGGCTTCAG AATGTATGGA TGCTGATTCT 
GCCTCCAGTT CTGAATCAGA GAGAAACATC ACTATCATGG CTTCAGGGAG CACAGGTGGT 
GAAAAAGATG GCCTTCGGAA TAGCACTGGA CTTGGTTCCC AAAACAAGTT TGTGGTTGGT 
AGCAGCAGCA ATAATGTGGG CCATGGAAGT AGTCCTGGGC CATGGGGTTT TTCCCATGGA 
GCCATAATAA GCACATGTCA GGTCTCTGTG GATGCTCCTG AAAGCAAATC AGAAAGTAGC 
AACAATAGAA TGAATGCTTG GGGCACTGTA AGTTCTTCAT CAAATGGAGG GTTAAATCCA 
AGCACTTTGA ATTCAGCTAG CAACCATGGT GCCTGGCCAG TATTAGACAA CAACGGACTT 
GCCCTCAAAG GGCCTGTAGG GAGCGGTAGT TCTGGCATCA ATATTCAGTG TAGTACCATA 
GGCCAGATGC CTAACAATCA GAGTATTAAC TCTAAAGTGG GTGGTTCTTC CACCCATGGT 
ACCTGGGGAA GCCTTCAGGA AACTTGTGAA TCTGAAGTAA GTGGTACACA GAAGGTTTCA 
TTCAGTGGTC AACCTCAGAA TATTACCACT GAGATGACTG GACCAAATAA CACTACTAAC 
TTTATGACCT CTAGTTTACC AAACTCCGGT TCAGTACAGA ATAACGAACT GCCTAGTAAT 
ACAGGGGCCT GGCGTGTGAG CACAATGAAT CATCCTCAGA TACAGGCTCC ATCGGTTATG 
AATGGCACTT CCCTTTCTCA TCTTAGCAAT GGAGAGCCAA AAAGTGGAGG CTCTTACGGT 
ACTACGTGGG GTGCCTATGG TTCTAATTAC TCTGGAGACA AATGTTCGAG CCCTAACGGC 
CAAGCTAATG GTGACACTGT GAATGCAACT CTAATGCAGC CTGGCATAAA TGGGCCTATG 
GGCACTAACT TTCAAGTGAA CACAAATAAA GGAGGAGGTG TGTGGGAGTC TGGGGCAACG 
AATTCCCAGA ATGCATCGTG GGGAAGTGGA AATGGCGCAA ATTCTGGAGG AAGTCGACGA 
GGATGGGGAA CTCCTGCACA AAACACTGGC ACTAATATAC CCAGCGTGGA GTGGAGCAAA 
CTGCCTAGCA ATCAGCATTC CAATGACAGT GCAAATGGCA ATGGTAAGAA GTTTACAAAT 
GGATGGAAAT CTACTGAGGA AGAGGATCAG GCTTCTGCCG CATCTCAGAC AACTGAGCAG 
AACAGTGTGT GGGCCAAAAC AGGAGGTACA GTGGAGAGTG AAGGTAGTAC AGAAAGCACT 
GGACGCCTCG AGGAGAAAGC CACTGGGGAA AATCAGAGTA GAGATCGGAG AAAAATTGAT 
CAGCACACAT TACTCCAAAG CATCGTAAAC AGAACTGACT TGGATCCACG TGTCCTCTCC 
AACTCTGGTT GGGGACAGAC TCCTATTAAG CAGAATACTG CCTGGGATAC GGAAACATCA 
CCTAGAGGGG AAAGAAAGAC TGACAATGGG ACAGAGGCCT GGGGAAGCTC TGCAACACAG 
ACTTTTAACT CAGGGGCATG TACAGATAAG ACTAGCCCTA CTAGTAATGA TACCTCATCT 
GTATCGGGGT GGGGAGATCC CAAACCTGCT CTGAGGTGGG GAGATTCCAA AGGCTCAAGC 
TGCCAGGGGG GATGGGAAGA TGATGCTGCT GCTACAGGAA TGGTCAAGAG CAATCAGTGG 
GGGAACAGCA AAGAAGAGAA GTCCGCATGG AATGATTCGC AAAAGAGCAA ACAGGGATGG 
GGTGATGGAC AGAAATCAAA CCAAGGGTGG TCCGTTTCTG CCAGTGATAA CTGGGGAGAA 
ACGTCAAGGA GTAACCATTG GGGTGAGGCT AATAAGAAAT CTAGCTCAGG AGGTAGTGAC 
AGTGACAGGT CTGTTTCTGG TTGGAATGAA CTTGGTAAAA CTAGTTCTTT TACTTGGGGA 
AATAACATAA ATCCAAATAA TTCGTCAGGA TGGGATGAAT CTTCTAAACC TAATCCTTCC 
CAGGGATGGG GAGATCCTCC AAAGTCTAAT CAGTCTCTAG GTTGGGGAGA TTCGTCAAAG 
CCAGTCACTT CTCCAGACTG GAACAAGCAA CAAGACATTG TCGGGTCTTG GGGAATCCCA 
CCAGCAACAG GCAAACCTCC TGGTACAGGC TGGCTGGGAG GACCTATACC AGCCCCAACA 
AAAGAAGAAG AGCCCACAGG CTGGGAGGAA CCATCCCCAG AATCCATACG TCGCAAAATG 
GAGATCGATG ACGGAACTTC AGCTTGGGGA GATCCGAGCA AATACAACTA CAAAAATGTG 
AACATGTGGA ACAAAAATGT CCCAAATGGC AGCAGCCGTT CAGACCAGCA AGCACAGGTA 
CACCAGTTGC TACCATCTGC AAGTGCCATC TCAAACAAAG AGGCAGGCAG TGGCTCTGGG 
CCAAAATCTA TGCAAGATGG CTGGTGTGGT GATGATATGC CATTGCCTGG AAATCGCCCC 
ACTGGCTGGG AAGAGGAAGA GGATGTAGAG ATTGGAATGT GGAATAGTAA TTCATCTCAA 
GAGCTTAACT CATCTTTAAA TTGGCCTCCA TATACAAAGA AAATGTCATC GAAGGGTCTG 
AGTGGCAAAA AAAGGAGAAG GGAAAGGGGA ACCATGAAAG GTGGAAACAA ACAAGAAGAA 
GCATGGATAA ATCCATTCGT TAAACAGTTT TCAAATATCA GTTTTTCGAG AGACTCACCC 
GAAGAAAATG TACAGAGCAA TAAGATGGAT CTTTCCGGAG GAATGTTACA GGACAAACGA 
ATGGAGATAG ATAAACATAG CCTAAATATT GGTGATTACA ATCGAACGGT CGGGAAAGGC 
CCTGGTTCTC GGCCTCAGAT TTCCAAAGAG TCTTCCATGG AGCGCAGCCC TTACTTTGAT 
AAGGATGGCA TTGTAGCAGA TGAATCCCAA AACATGCAGT TTATGTCCAG TCAAAGCATG 
AAGCTTCCCC CTTCAAATAG TGCACTACCT AACCAGGCCC TTGGCTCCAT AGCAGGGCTG 
GGTATGCAAA ACTTGAATTC TGTTAGACAG AATGGCAATC CCAGTATGTT TGGTGTTGGA 
AACACAGCAG CACAACCCCG GGGCATGCAG CAGCCTCCAG CACAACCTCT TAGTTCATCT 
CAGCCTAATC TTCGTGCTCA AGTGCCTCCT CCATTACTCT CCCCTCAGGT TCCAGTTTCA 
TTGCTGAAGT ATGCACCAAA CAACGGTGGC CTGAATCCGC TCTTTGGCCC TCAACAGGTA 
GCCATGCTGA ACCAGCTATC CCAACTAAAC CAGCTTTCTC AGATCTCCCA GTTACAGAGA 
TTGTTAGCGC AGCAGCAAAG GGCGCAGAGT CAGAGAAGCG TGCCTTCTGG GAACCGGCAG 
CAGCAAGACC AGCAGGGTCG ACCTCTTAGC GTGCAGCAGC AGATGATGCA GCAGTCCCGG 
CAACTCGATC CCAGCCTGTT GGTGAAGCAG CAGACCCCGC CATCTCAGCA GCAGCCACTC 
CATCAGCCAG CCATGAAATC CTTCCTCGAG AATGTCATGC CCCACACTAC ACCTGAGCTG 
CAGAAAGGGC CATCACCAAT AAATGCTTTC AGCAACTTCC CGATAGGCTT GAACTCAAAC 
TTGAATGTAA ATATGGATAT GAACAGTATT AAAGAGCCAC AGTCAAGACT AAGGAAGTGG 
ACTACAGTGG ACAGCATTTC TGTGAACACA TCTTTGGATC AAAACTCCAG CAAACATGGT 
GCTATTTCAA GTGGTTTCAG GCTGGAAGAG TCTCCATTTG TTCCCTATGA CTTTATGAAC 
AGCAGTACTT CTCCAGCCAG CCCTCCGGGT TCAATAGGAG ATGGCTGGCC ACGTGCCAAA 
TCGCCTAACG GCTCTAGCAG TGTTAACTGG CCACCAGAAT TTCGCCCTGG TGAACCATGG 
AAAGGTTATC CAAACATTGA CCCTGAAACT GACCCTTACG TCACTCCTGG CAGTGTCATA 
AACAATCTTT CAATTAATAC TGTGCGGGAA GTTGACCACC TCAGGGACAG GAACAGTGGG 
TCATCCTCAT CCTTGAACAC CACGCTGCCT TCAACTAGTG CCTGGTCATC CATTCGTGCC 
TCCAACTACA ACGTTCCCCT CAGCAGTACA GCACAAAGCA CTTCAGCCAG AAATAGTGAT 
TCCAAATTGA CATGGTCTCC TGGTTCAGTT ACAAACACCT CTCTGGCTCA TGAGCTGTGG 
AAGGTCCCTT TACCACCTAA AAACATCACT GCTCCGTCCC GCCCACCTCC GGGACTCACT 
GGTCAGAAGC CACCCTTGTC TACGTGGGAT AACTCTCCCC TTCGTGTAGG TGGAGGATGG 
GGAAATTCTG ACGCCAGGTA CACCCCAGGT TCCAGTTGGG GTGAGAGCAG CTCAGGGAGA 
ATAACAAATT GGCTTGTTCT AAAGAACCTC ACACCTCAGA TTGACGGCTC AACTCTGCGT 
ACTCTGTGCA TGCAGCATGG CCCACTGATA ACATTCCACC TGAACCTCCC ACATGGAAAT 
GCTTTGGTCC GTTACAGTTC AAAAGAAGAG GTAGTGAAGG CACAAAAGTC TCTGCACATG 
TGTGTATTGG GGAACACTAC TATTCTTGCT GAGTTTGCCA GTGAAGAGGA GATCAGCCGT 
TTCTTTGCAC AAAGTCAGTC TCTGACCCCT TCTCCCGGCT GGCAGTCTCT CGGGTCCAGC 
CAGAGCCGGC TGGGCTCCCT CGACTGTTCC CACTCGTTCT CCAGCCGGAC CGATCTCAAT 
CACTGGAATG GTGCTGGGCT GTCGGGAACT AACTGTGGAG ACCTTCACGG CACTTCACTC 
TGGGGGACCC CACATTATTC CACAAGCCTG TGGGGTCCTC CCAGCAGCAG CGACCCCCGG 
GGAATTAGCA GCCCATCCCC CATTAATGCT TTTCTTTCTG TTGACCACCT GGGTGGGGGT 
GGAGAGTCCA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_022419938.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022275646.1 Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X8, mRNA.

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CloneID	OCe288917
Clone ID Related Accession (Same CDS sequence)	XM_022419938.1
Accession Version	XM_022419938.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	trinucleotide repeat-containing gene 6A protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACTAG TGGCAGAGCC AGGCCTAGAA GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCC AGTGTAAGCC AGCCTCAGCC TGCCAACTCT 
AATAACGGCA CTTCCACAGC AACCAGCACT AATAATAATG CCAAGCGAGC CACAGCCAAC 
AATCCGCAGC AGCAGCAGCA GCCACAGCCG CCACAGCAGC CACAGCAGCC GCCGCAGCAG 
CCACCGCAGC CGCCACAGCA GCAGCCGCAG CCAGCACAGG CCTTGCCTCG GTATCCTCGG 
GAAGTACCAC CACGATTTCG CCACCAGGAA CACAAACAGC TTCTAAAGAG GGGTCAGCAT 
TTTCCTGTCA TAGCAGCAAA CCTGGGATCT GCTGTTAAAG TGTTAAGCAG CCAGTCAGAA 
AGCAGTGCTT TAACAAATCA ACAGCCACAA AATAACGGAG AGGTGCAGAA CAGCAAAAAC 
CAGTCAGATA TAAACCACAG TACTTCAGGA TCCCATTATG AAAATTCACA GCGGGGACCT 
GTGTCTTCTC CGAGTGACTC TAGCACAAAT TGTAAGAATG CTGTTGTAAA CGACTCCCCT 
GAGACAGAAG CATGGCCCTC AGTCCCTGGC AGTGATCCAG AGTTGGCTTC AGAATGTATG 
GATGCTGATT CTGCCTCCAG TTCTGAATCA GAGAGAAACA TCACTATCAT GGCTTCAGGG 
AGCACAGGTG GTGAAAAAGA TGGCCTTCGG AATAGCACTG GACTTGGTTC CCAAAACAAG 
TTTGTGGTTG GTAGCAGCAG CAATAATGTG GGCCATGGAA GTAGTCCTGG GCCATGGGGT 
TTTTCCCATG GAGCCATAAT AAGCACATGT CAGGTCTCTG TGGATGCTCC TGAAAGCAAA 
TCAGAAAGTA GCAACAATAG AATGAATGCT TGGGGCACTG TAAGTTCTTC ATCAAATGGA 
GGGTTAAATC CAAGCACTTT GAATTCAGCT AGCAACCATG GTGCCTGGCC AGTATTAGAC 
AACAACGGAC TTGCCCTCAA AGGGCCTGTA GGGAGCGGTA GTTCTGGCAT CAATATTCAG 
TGTAGTACCA TAGGCCAGAT GCCTAACAAT CAGAGTATTA ACTCTAAAGT GGGTGGTTCT 
TCCACCCATG GTACCTGGGG AAGCCTTCAG GAAACTTGTG AATCTGAAGT AAGTGGTACA 
CAGAAGGTTT CATTCAGTGG TCAACCTCAG AATATTACCA CTGAGATGAC TGGACCAAAT 
AACACTACTA ACTTTATGAC CTCTAGTTTA CCAAACTCCG GTTCAGTACA GAATAACGAA 
CTGCCTAGTA ATACAGGGGC CTGGCGTGTG AGCACAATGA ATCATCCTCA GATACAGGCT 
CCATCGGTTA TGAATGGCAC TTCCCTTTCT CATCTTAGCA ATGGAGAGCC AAAAAGTGGA 
GGCTCTTACG GTACTACGTG GGGTGCCTAT GGTTCTAATT ACTCTGGAGA CAAATGTTCG 
AGCCCTAACG GCCAAGCTAA TGGTGACACT GTGAATGCAA CTCTAATGCA GCCTGGCATA 
AATGGGCCTA TGGGCACTAA CTTTCAAGTG AACACAAATA AAGGAGGAGG TGTGTGGGAG 
TCTGGGGCAA CGAATTCCCA GAATGCATCG TGGGGAAGTG GAAATGGCGC AAATTCTGGA 
GGAAGTCGAC GAGGATGGGG AACTCCTGCA CAAAACACTG GCACTAATAT ACCCAGCGTG 
GAGTGGAGCA AACTGCCTAG CAATCAGCAT TCCAATGACA GTGCAAATGG CAATGGTAAG 
AAGTTTACAA ATGGATGGAA ATCTACTGAG GAAGAGGATC AGGCTTCTGC CGCATCTCAG 
ACAACTGAGC AGAACAGTGT GTGGGCCAAA ACAGGAGGTA CAGTGGAGAG TGAAGGTAGT 
ACAGAAAGCA CTGGACGCCT CGAGGAGAAA GCCACTGGGG AAAATCAGAG TAGAGATCGG 
AGAAAAATTG ATCAGCACAC ATTACTCCAA AGCATCGTAA ACAGAACTGA CTTGGATCCA 
CGTGTCCTCT CCAACTCTGG TTGGGGACAG ACTCCTATTA AGCAGAATAC TGCCTGGGAT 
ACGGAAACAT CACCTAGAGG GGAAAGAAAG ACTGACAATG GGACAGAGGC CTGGGGAAGC 
TCTGCAACAC AGACTTTTAA CTCAGGGGCA TGTACAGATA AGACTAGCCC TACTAGTAAT 
GATACCTCAT CTGTATCGGG GTGGGGAGAT CCCAAACCTG CTCTGAGGTG GGGAGATTCC 
AAAGGCTCAA GCTGCCAGGG GGGATGGGAA GATGATGCTG CTGCTACAGG AATGGTCAAG 
AGCAATCAGT GGGGGAACAG CAAAGAAGAG AAGTCCGCAT GGAATGATTC GCAAAAGAGC 
AAACAGGGAT GGGGTGATGG ACAGAAATCA AACCAAGGGT GGTCCGTTTC TGCCAGTGAT 
AACTGGGGAG AAACGTCAAG GAGTAACCAT TGGGGTGAGG CTAATAAGAA ATCTAGCTCA 
GGAGGTAGTG ACAGTGACAG GTCTGTTTCT GGTTGGAATG AACTTGGTAA AACTAGTTCT 
TTTACTTGGG GAAATAACAT AAATCCAAAT AATTCGTCAG GATGGGATGA ATCTTCTAAA 
CCTAATCCTT CCCAGGGATG GGGAGATCCT CCAAAGTCTA ATCAGTCTCT AGGTTGGGGA 
GATTCGTCAA AGCCAGTCAC TTCTCCAGAC TGGAACAAGC AACAAGACAT TGTCGGGTCT 
TGGGGAATCC CACCAGCAAC AGGCAAACCT CCTGGTACAG GCTGGCTGGG AGGACCTATA 
CCAGCCCCAA CAAAAGAAGA AGAGCCCACA GGCTGGGAGG AACCATCCCC AGAATCCATA 
CGTCGCAAAA TGGAGATCGA TGACGGAACT TCAGCTTGGG GAGATCCGAG CAAATACAAC 
TACAAAAATG TGAACATGTG GAACAAAAAT GTCCCAAATG GCAGCAGCCG TTCAGACCAG 
CAAGCACAGG TACACCAGTT GCTACCATCT GCAAGTGCCA TCTCAAACAA AGAGGCAGGC 
AGTGGCTCTG GCTGGGGTGA GCCCTGGGGG GAGCCTTCTA CTCCAGCCAC AACTGTGGAT 
AATGGTACTT CAGCATGGGG TAAACCCATA GACAGTGGTC CCAGCTGGGG GGAGCCCATT 
GCTGCAGCAT CCAACACATC CACGTGGGGC TCCAGCTCTG TTGGTCCTCA AGCATTAAGC 
AAATCTGGGC CAAAATCTAT GCAAGATGGC TGGTGTGGTG ATGATATGCC ATTGCCTGGA 
AATCGCCCCA CTGGCTGGGA AGAGGAAGAG GATGTAGAGA TTGGAATGTG GAATAGTAAT 
TCATCTCAAG AGCTTAACTC ATCTTTAAAT TGGCCTCCAT ATACAAAGAA AATGTCATCG 
AAGGGTCTGA GTGGCAAAAA AAGGAGAAGG GAAAGGGGAA CCATGAAAGG TGGAAACAAA 
CAAGAAGAAG CATGGATAAA TCCATTCGTT AAACAGTTTT CAAATATCAG TTTTTCGAGA 
GACTCACCCG AAGAAAATGT ACAGAGCAAT AAGATGGATC TTTCCGGAGG AATGTTACAG 
GACAAACGAA TGGAGATAGA TAAACATAGC CTAAATATTG GTGATTACAA TCGAACGGTC 
GGGAAAGGCC CTGGTTCTCG GCCTCAGATT TCCAAAGAGT CTTCCATGGA GCGCAGCCCT 
TACTTTGATA AGAATGGCAA TCCCAGTATG TTTGGTGTTG GAAACACAGC AGCACAACCC 
CGGGGCATGC AGCAGCCTCC AGCACAACCT CTTAGTTCAT CTCAGCCTAA TCTTCGTGCT 
CAAGTGCCTC CTCCATTACT CTCCCCTCAG GTTCCAGTTT CATTGCTGAA GTATGCACCA 
AACAACGGTG GCCTGAATCC GCTCTTTGGC CCTCAACAGG TAGCCATGCT GAACCAGCTA 
TCCCAACTAA ACCAGCTTTC TCAGATCTCC CAGTTACAGA GATTGTTAGC GCAGCAGCAA 
AGGGCGCAGA GTCAGAGAAG CGTGCCTTCT GGGAACCGGC AGCAGCAAGA CCAGCAGGGT 
CGACCTCTTA GCGTGCAGCA GCAGATGATG CAGCAGTCCC GGCAACTCGA TCCCAGCCTG 
TTGGTGAAGC AGCAGACCCC GCCATCTCAG CAGCAGCCAC TCCATCAGCC AGCCATGAAA 
TCCTTCCTCG AGAATGTCAT GCCCCACACT ACACCTGAGC TGCAGAAAGG GCCATCACCA 
ATAAATGCTT TCAGCAACTT CCCGATAGGC TTGAACTCAA ACTTGAATGT AAATATGGAT 
ATGAACAGTA TTAAAGAGCC ACAGTCAAGA CTAAGGAAGT GGACTACAGT GGACAGCATT 
TCTGTGAACA CATCTTTGGA TCAAAACTCC AGCAAACATG GTGCTATTTC AAGTGGTTTC 
AGGCTGGAAG AGTCTCCATT TGTTCCCTAT GACTTTATGA ACAGCAGTAC TTCTCCAGCC 
AGCCCTCCGG GTTCAATAGG AGATGGCTGG CCACGTGCCA AATCGCCTAA CGGCTCTAGC 
AGTGTTAACT GGCCACCAGA ATTTCGCCCT GGTGAACCAT GGAAAGGTTA TCCAAACATT 
GACCCTGAAA CTGACCCTTA CGTCACTCCT GGCAGTGTCA TAAACAATCT TTCAATTAAT 
ACTGTGCGGG AAGTTGACCA CCTCAGGGAC AGGAACAGTG GGTCATCCTC ATCCTTGAAC 
ACCACGCTGC CTTCAACTAG TGCCTGGTCA TCCATTCGTG CCTCCAACTA CAACGTTCCC 
CTCAGCAGTA CAGCACAAAG CACTTCAGCC AGAAATAGTG ATTCCAAATT GACATGGTCT 
CCTGGTTCAG TTACAAACAC CTCTCTGGCT CATGAGCTGT GGAAGGTCCC TTTACCACCT 
AAAAACATCA CTGCTCCGTC CCGCCCACCT CCGGGACTCA CTGGTCAGAA GCCACCCTTG 
TCTACGTGGG ATAACTCTCC CCTTCGTGTA GGTGGAGGAT GGGGAAATTC TGACGCCAGG 
TACACCCCAG GTTCCAGTTG GGGTGAGAGC AGCTCAGGGA GAATAACAAA TTGGCTTGTT 
CTAAAGAACC TCACACCTCA GATTGACGGC TCAACTCTGC GTACTCTGTG CATGCAGCAT 
GGCCCACTGA TAACATTCCA CCTGAACCTC CCACATGGAA ATGCTTTGGT CCGTTACAGT 
TCAAAAGAAG AGGTAGTGAA GGCACAAAAG TCTCTGCACA TGTGTGTATT GGGGAACACT 
ACTATTCTTG CTGAGTTTGC CAGTGAAGAG GAGATCAGCC GTTTCTTTGC ACAAAGTCAG 
TCTCTGACCC CTTCTCCCGG CTGGCAGTCT CTCGGGTCCA GCCAGAGCCG GCTGGGCTCC 
CTCGACTGTT CCCACTCGTT CTCCAGCCGG ACCGATCTCA ATCACTGGAA TGGTGCTGGG 
CTGTCGGGAA CTAACTGTGG AGACCTTCAC GGCACTTCAC TCTGGGGGAC CCCACATTAT 
TCCACAAGCC TGTGGGGTCC TCCCAGCAGC AGCGACCCCC GGGGAATTAG CAGCCCATCC 
CCCATTAATG CTTTTCTTTC TGTTGACCAC CTGGGTGGGG GTGGAGAGTC CATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022275646.1
CDS	275..6031
Translation	MQLVAEPGLEARCPGSQDIPLPEEWNRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNPQQQQQPQPPQQPQQPPQQPPQPPQQQPQPAQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLSSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSPSDSSTNCKNAVVNDSPETEAWPSVPGSDPELASECMDADSASSSESERNITIMASGSTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSPGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLDNNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVGGSSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVMNGTSLSHLSNGEPKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGINGPMGTNFQVNTNKGGGVWESGATNSQNASWGSGNGANSGGSRRGWGTPAQNTGTNIPSVEWSKLPSNQHSNDSANGNGKKFTNGWKSTEEEDQASAASQTTEQNSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACTDKTSPTSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDAAATGMVKSNQWGNSKEEKSAWNDSQKSKQGWGDGQKSNQGWSVSASDNWGETSRSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVTSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPTKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSASAISNKEAGSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASNTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGTMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERSPYFDKNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPSLLVKQQTPPSQQQPLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_022419938.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022419938.1

ORF Insert Sequence:

ATGCAACTAG TGGCAGAGCC AGGCCTAGAA GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCC AGTGTAAGCC AGCCTCAGCC TGCCAACTCT 
AATAACGGCA CTTCCACAGC AACCAGCACT AATAATAATG CCAAGCGAGC CACAGCCAAC 
AATCCGCAGC AGCAGCAGCA GCCACAGCCG CCACAGCAGC CACAGCAGCC GCCGCAGCAG 
CCACCGCAGC CGCCACAGCA GCAGCCGCAG CCAGCACAGG CCTTGCCTCG GTATCCTCGG 
GAAGTACCAC CACGATTTCG CCACCAGGAA CACAAACAGC TTCTAAAGAG GGGTCAGCAT 
TTTCCTGTCA TAGCAGCAAA CCTGGGATCT GCTGTTAAAG TGTTAAGCAG CCAGTCAGAA 
AGCAGTGCTT TAACAAATCA ACAGCCACAA AATAACGGAG AGGTGCAGAA CAGCAAAAAC 
CAGTCAGATA TAAACCACAG TACTTCAGGA TCCCATTATG AAAATTCACA GCGGGGACCT 
GTGTCTTCTC CGAGTGACTC TAGCACAAAT TGTAAGAATG CTGTTGTAAA CGACTCCCCT 
GAGACAGAAG CATGGCCCTC AGTCCCTGGC AGTGATCCAG AGTTGGCTTC AGAATGTATG 
GATGCTGATT CTGCCTCCAG TTCTGAATCA GAGAGAAACA TCACTATCAT GGCTTCAGGG 
AGCACAGGTG GTGAAAAAGA TGGCCTTCGG AATAGCACTG GACTTGGTTC CCAAAACAAG 
TTTGTGGTTG GTAGCAGCAG CAATAATGTG GGCCATGGAA GTAGTCCTGG GCCATGGGGT 
TTTTCCCATG GAGCCATAAT AAGCACATGT CAGGTCTCTG TGGATGCTCC TGAAAGCAAA 
TCAGAAAGTA GCAACAATAG AATGAATGCT TGGGGCACTG TAAGTTCTTC ATCAAATGGA 
GGGTTAAATC CAAGCACTTT GAATTCAGCT AGCAACCATG GTGCCTGGCC AGTATTAGAC 
AACAACGGAC TTGCCCTCAA AGGGCCTGTA GGGAGCGGTA GTTCTGGCAT CAATATTCAG 
TGTAGTACCA TAGGCCAGAT GCCTAACAAT CAGAGTATTA ACTCTAAAGT GGGTGGTTCT 
TCCACCCATG GTACCTGGGG AAGCCTTCAG GAAACTTGTG AATCTGAAGT AAGTGGTACA 
CAGAAGGTTT CATTCAGTGG TCAACCTCAG AATATTACCA CTGAGATGAC TGGACCAAAT 
AACACTACTA ACTTTATGAC CTCTAGTTTA CCAAACTCCG GTTCAGTACA GAATAACGAA 
CTGCCTAGTA ATACAGGGGC CTGGCGTGTG AGCACAATGA ATCATCCTCA GATACAGGCT 
CCATCGGTTA TGAATGGCAC TTCCCTTTCT CATCTTAGCA ATGGAGAGCC AAAAAGTGGA 
GGCTCTTACG GTACTACGTG GGGTGCCTAT GGTTCTAATT ACTCTGGAGA CAAATGTTCG 
AGCCCTAACG GCCAAGCTAA TGGTGACACT GTGAATGCAA CTCTAATGCA GCCTGGCATA 
AATGGGCCTA TGGGCACTAA CTTTCAAGTG AACACAAATA AAGGAGGAGG TGTGTGGGAG 
TCTGGGGCAA CGAATTCCCA GAATGCATCG TGGGGAAGTG GAAATGGCGC AAATTCTGGA 
GGAAGTCGAC GAGGATGGGG AACTCCTGCA CAAAACACTG GCACTAATAT ACCCAGCGTG 
GAGTGGAGCA AACTGCCTAG CAATCAGCAT TCCAATGACA GTGCAAATGG CAATGGTAAG 
AAGTTTACAA ATGGATGGAA ATCTACTGAG GAAGAGGATC AGGCTTCTGC CGCATCTCAG 
ACAACTGAGC AGAACAGTGT GTGGGCCAAA ACAGGAGGTA CAGTGGAGAG TGAAGGTAGT 
ACAGAAAGCA CTGGACGCCT CGAGGAGAAA GCCACTGGGG AAAATCAGAG TAGAGATCGG 
AGAAAAATTG ATCAGCACAC ATTACTCCAA AGCATCGTAA ACAGAACTGA CTTGGATCCA 
CGTGTCCTCT CCAACTCTGG TTGGGGACAG ACTCCTATTA AGCAGAATAC TGCCTGGGAT 
ACGGAAACAT CACCTAGAGG GGAAAGAAAG ACTGACAATG GGACAGAGGC CTGGGGAAGC 
TCTGCAACAC AGACTTTTAA CTCAGGGGCA TGTACAGATA AGACTAGCCC TACTAGTAAT 
GATACCTCAT CTGTATCGGG GTGGGGAGAT CCCAAACCTG CTCTGAGGTG GGGAGATTCC 
AAAGGCTCAA GCTGCCAGGG GGGATGGGAA GATGATGCTG CTGCTACAGG AATGGTCAAG 
AGCAATCAGT GGGGGAACAG CAAAGAAGAG AAGTCCGCAT GGAATGATTC GCAAAAGAGC 
AAACAGGGAT GGGGTGATGG ACAGAAATCA AACCAAGGGT GGTCCGTTTC TGCCAGTGAT 
AACTGGGGAG AAACGTCAAG GAGTAACCAT TGGGGTGAGG CTAATAAGAA ATCTAGCTCA 
GGAGGTAGTG ACAGTGACAG GTCTGTTTCT GGTTGGAATG AACTTGGTAA AACTAGTTCT 
TTTACTTGGG GAAATAACAT AAATCCAAAT AATTCGTCAG GATGGGATGA ATCTTCTAAA 
CCTAATCCTT CCCAGGGATG GGGAGATCCT CCAAAGTCTA ATCAGTCTCT AGGTTGGGGA 
GATTCGTCAA AGCCAGTCAC TTCTCCAGAC TGGAACAAGC AACAAGACAT TGTCGGGTCT 
TGGGGAATCC CACCAGCAAC AGGCAAACCT CCTGGTACAG GCTGGCTGGG AGGACCTATA 
CCAGCCCCAA CAAAAGAAGA AGAGCCCACA GGCTGGGAGG AACCATCCCC AGAATCCATA 
CGTCGCAAAA TGGAGATCGA TGACGGAACT TCAGCTTGGG GAGATCCGAG CAAATACAAC 
TACAAAAATG TGAACATGTG GAACAAAAAT GTCCCAAATG GCAGCAGCCG TTCAGACCAG 
CAAGCACAGG TACACCAGTT GCTACCATCT GCAAGTGCCA TCTCAAACAA AGAGGCAGGC 
AGTGGCTCTG GCTGGGGTGA GCCCTGGGGG GAGCCTTCTA CTCCAGCCAC AACTGTGGAT 
AATGGTACTT CAGCATGGGG TAAACCCATA GACAGTGGTC CCAGCTGGGG GGAGCCCATT 
GCTGCAGCAT CCAACACATC CACGTGGGGC TCCAGCTCTG TTGGTCCTCA AGCATTAAGC 
AAATCTGGGC CAAAATCTAT GCAAGATGGC TGGTGTGGTG ATGATATGCC ATTGCCTGGA 
AATCGCCCCA CTGGCTGGGA AGAGGAAGAG GATGTAGAGA TTGGAATGTG GAATAGTAAT 
TCATCTCAAG AGCTTAACTC ATCTTTAAAT TGGCCTCCAT ATACAAAGAA AATGTCATCG 
AAGGGTCTGA GTGGCAAAAA AAGGAGAAGG GAAAGGGGAA CCATGAAAGG TGGAAACAAA 
CAAGAAGAAG CATGGATAAA TCCATTCGTT AAACAGTTTT CAAATATCAG TTTTTCGAGA 
GACTCACCCG AAGAAAATGT ACAGAGCAAT AAGATGGATC TTTCCGGAGG AATGTTACAG 
GACAAACGAA TGGAGATAGA TAAACATAGC CTAAATATTG GTGATTACAA TCGAACGGTC 
GGGAAAGGCC CTGGTTCTCG GCCTCAGATT TCCAAAGAGT CTTCCATGGA GCGCAGCCCT 
TACTTTGATA AGAATGGCAA TCCCAGTATG TTTGGTGTTG GAAACACAGC AGCACAACCC 
CGGGGCATGC AGCAGCCTCC AGCACAACCT CTTAGTTCAT CTCAGCCTAA TCTTCGTGCT 
CAAGTGCCTC CTCCATTACT CTCCCCTCAG GTTCCAGTTT CATTGCTGAA GTATGCACCA 
AACAACGGTG GCCTGAATCC GCTCTTTGGC CCTCAACAGG TAGCCATGCT GAACCAGCTA 
TCCCAACTAA ACCAGCTTTC TCAGATCTCC CAGTTACAGA GATTGTTAGC GCAGCAGCAA 
AGGGCGCAGA GTCAGAGAAG CGTGCCTTCT GGGAACCGGC AGCAGCAAGA CCAGCAGGGT 
CGACCTCTTA GCGTGCAGCA GCAGATGATG CAGCAGTCCC GGCAACTCGA TCCCAGCCTG 
TTGGTGAAGC AGCAGACCCC GCCATCTCAG CAGCAGCCAC TCCATCAGCC AGCCATGAAA 
TCCTTCCTCG AGAATGTCAT GCCCCACACT ACACCTGAGC TGCAGAAAGG GCCATCACCA 
ATAAATGCTT TCAGCAACTT CCCGATAGGC TTGAACTCAA ACTTGAATGT AAATATGGAT 
ATGAACAGTA TTAAAGAGCC ACAGTCAAGA CTAAGGAAGT GGACTACAGT GGACAGCATT 
TCTGTGAACA CATCTTTGGA TCAAAACTCC AGCAAACATG GTGCTATTTC AAGTGGTTTC 
AGGCTGGAAG AGTCTCCATT TGTTCCCTAT GACTTTATGA ACAGCAGTAC TTCTCCAGCC 
AGCCCTCCGG GTTCAATAGG AGATGGCTGG CCACGTGCCA AATCGCCTAA CGGCTCTAGC 
AGTGTTAACT GGCCACCAGA ATTTCGCCCT GGTGAACCAT GGAAAGGTTA TCCAAACATT 
GACCCTGAAA CTGACCCTTA CGTCACTCCT GGCAGTGTCA TAAACAATCT TTCAATTAAT 
ACTGTGCGGG AAGTTGACCA CCTCAGGGAC AGGAACAGTG GGTCATCCTC ATCCTTGAAC 
ACCACGCTGC CTTCAACTAG TGCCTGGTCA TCCATTCGTG CCTCCAACTA CAACGTTCCC 
CTCAGCAGTA CAGCACAAAG CACTTCAGCC AGAAATAGTG ATTCCAAATT GACATGGTCT 
CCTGGTTCAG TTACAAACAC CTCTCTGGCT CATGAGCTGT GGAAGGTCCC TTTACCACCT 
AAAAACATCA CTGCTCCGTC CCGCCCACCT CCGGGACTCA CTGGTCAGAA GCCACCCTTG 
TCTACGTGGG ATAACTCTCC CCTTCGTGTA GGTGGAGGAT GGGGAAATTC TGACGCCAGG 
TACACCCCAG GTTCCAGTTG GGGTGAGAGC AGCTCAGGGA GAATAACAAA TTGGCTTGTT 
CTAAAGAACC TCACACCTCA GATTGACGGC TCAACTCTGC GTACTCTGTG CATGCAGCAT 
GGCCCACTGA TAACATTCCA CCTGAACCTC CCACATGGAA ATGCTTTGGT CCGTTACAGT 
TCAAAAGAAG AGGTAGTGAA GGCACAAAAG TCTCTGCACA TGTGTGTATT GGGGAACACT 
ACTATTCTTG CTGAGTTTGC CAGTGAAGAG GAGATCAGCC GTTTCTTTGC ACAAAGTCAG 
TCTCTGACCC CTTCTCCCGG CTGGCAGTCT CTCGGGTCCA GCCAGAGCCG GCTGGGCTCC 
CTCGACTGTT CCCACTCGTT CTCCAGCCGG ACCGATCTCA ATCACTGGAA TGGTGCTGGG 
CTGTCGGGAA CTAACTGTGG AGACCTTCAC GGCACTTCAC TCTGGGGGAC CCCACATTAT 
TCCACAAGCC TGTGGGGTCC TCCCAGCAGC AGCGACCCCC GGGGAATTAG CAGCCCATCC 
CCCATTAATG CTTTTCTTTC TGTTGACCAC CTGGGTGGGG GTGGAGAGTC CATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_022419939.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022275647.1 Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X10, mRNA.

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CloneID	OCe288918
Clone ID Related Accession (Same CDS sequence)	XM_022419939.1
Accession Version	XM_022419939.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	trinucleotide repeat-containing gene 6A protein isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACTAG TGGCAGAGCC AGGCCTAGAA GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG AGAATTGGAA GCTAAAGCTA CCAAAGACGT AGAAAGGACT 
CTTAGCAGGG ATTTAGTGCA AGAAGAAGAA CAGTTGATGG AAGAAAAGAA AAAGAAAAAA 
GACGACAAGA AAAAGAAGGA AGCTGCTCAA AAGAAGGCCA CTGAACAAAA AATCAAAGTG 
CCAGAACAGA TAAAGCCCAG TGTAAGCCAG CCTCAGCCTG CCAACTCTAA TAACGGCACT 
TCCACAGCAA CCAGCACTAA TAATAATGCC AAGCGAGCCA CAGCCAACAA TCCGCAGCAG 
CAGCAGCAGC CACAGCCGCC ACAGCAGCCA CAGCAGCCGC CGCAGCAGCC ACCGCAGCCG 
CCACAGCAGC AGCCGCAGCC AGCACAGGCC TTGCCTCGGT ATCCTCGGGA AGTACCACCA 
CGATTTCGCC ACCAGGAACA CAAACAGCTT CTAAAGAGGG GTCAGCATTT TCCTGTCATA 
GCAGCAAACC TGGGATCTGC TGTTAAAGTG TTAAGCAGCC AGTCAGAAAG CAGTGCTTTA 
ACAAATCAAC AGCCACAAAA TAACGGAGAG GTGCAGAACA GCAAAAACCA GTCAGATATA 
AACCACAGTA CTTCAGGATC CCATTATGAA AATTCACAGC GGGGACCTGT GTCTTCTCCG 
AGTGACTCTA GCACAAATTG TAAGAATGCT GTTGTAAACG ACTCCCCTGA GACAGAAGCA 
TGGCCCTCAG TCCCTGGCAG TGATCCAGAG TTGGCTTCAG AATGTATGGA TGCTGATTCT 
GCCTCCAGTT CTGAATCAGA GAGAAACATC ACTATCATGG CTTCAGGGAG CACAGGTGGT 
GAAAAAGATG GCCTTCGGAA TAGCACTGGA CTTGGTTCCC AAAACAAGTT TGTGGTTGGT 
AGCAGCAGCA ATAATGTGGG CCATGGAAGT AGTCCTGGGC CATGGGGTTT TTCCCATGGA 
GCCATAATAA GCACATGTCA GGTCTCTGTG GATGCTCCTG AAAGCAAATC AGAAAGTAGC 
AACAATAGAA TGAATGCTTG GGGCACTGTA AGTTCTTCAT CAAATGGAGG GTTAAATCCA 
AGCACTTTGA ATTCAGCTAG CAACCATGGT GCCTGGCCAG TATTAGACAA CAACGGACTT 
GCCCTCAAAG GGCCTGTAGG GAGCGGTAGT TCTGGCATCA ATATTCAGTG TAGTACCATA 
GGCCAGATGC CTAACAATCA GAGTATTAAC TCTAAAGTGG GTGGTTCTTC CACCCATGGT 
ACCTGGGGAA GCCTTCAGGA AACTTGTGAA TCTGAAGTAA GTGGTACACA GAAGGTTTCA 
TTCAGTGGTC AACCTCAGAA TATTACCACT GAGATGACTG GACCAAATAA CACTACTAAC 
TTTATGACCT CTAGTTTACC AAACTCCGGT TCAGTACAGA ATAACGAACT GCCTAGTAAT 
ACAGGGGCCT GGCGTGTGAG CACAATGAAT CATCCTCAGA TACAGGCTCC ATCGGTTATG 
AATGGCACTT CCCTTTCTCA TCTTAGCAAT GGAGAGCCAA AAAGTGGAGG CTCTTACGGT 
ACTACGTGGG GTGCCTATGG TTCTAATTAC TCTGGAGACA AATGTTCGAG CCCTAACGGC 
CAAGCTAATG GTGACACTGT GAATGCAACT CTAATGCAGC CTGGCATAAA TGGGCCTATG 
GGCACTAACT TTCAAGTGAA CACAAATAAA GGAGGAGGTG TGTGGGAGTC TGGGGCAACG 
AATTCCCAGA ATGCATCGTG GGGAAGTGGA AATGGCGCAA ATTCTGGAGG AAGTCGACGA 
GGATGGGGAA CTCCTGCACA AAACACTGGC ACTAATATAC CCAGCGTGGA GTGGAGCAAA 
CTGCCTAGCA ATCAGCATTC CAATGACAGT GCAAATGGCA ATGGTAAGAA GTTTACAAAT 
GGATGGAAAT CTACTGAGGA AGAGGATCAG GCTTCTGCCG CATCTCAGAC AACTGAGCAG 
AACAGTGTGT GGGCCAAAAC AGGAGGTACA GTGGAGAGTG AAGGTAGTAC AGAAAGCACT 
GGACGCCTCG AGGAGAAAGC CACTGGGGAA AATCAGAGTA GAGATCGGAG AAAAATTGAT 
CAGCACACAT TACTCCAAAG CATCGTAAAC AGAACTGACT TGGATCCACG TGTCCTCTCC 
AACTCTGGTT GGGGACAGAC TCCTATTAAG CAGAATACTG CCTGGGATAC GGAAACATCA 
CCTAGAGGGG AAAGAAAGAC TGACAATGGG ACAGAGGCCT GGGGAAGCTC TGCAACACAG 
ACTTTTAACT CAGGGGCATG TACAGATAAG ACTAGCCCTA CTAGTAATGA TACCTCATCT 
GTATCGGGGT GGGGAGATCC CAAACCTGCT CTGAGGTGGG GAGATTCCAA AGGCTCAAGC 
TGCCAGGGGG GATGGGAAGA TGATGCTGCT GCTACAGGAA TGGTCAAGAG CAATCAGTGG 
GGGAACAGCA AAGAAGAGAA GTCCGCATGG AATGATTCGC AAAAGAGCAA ACAGGGATGG 
GGTGATGGAC AGAAATCAAA CCAAGGGTGG TCCGTTTCTG CCAGTGATAA CTGGGGAGAA 
ACGTCAAGGA GTAACCATTG GGGTGAGGCT AATAAGAAAT CTAGCTCAGG AGGTAGTGAC 
AGTGACAGGT CTGTTTCTGG TTGGAATGAA CTTGGTAAAA CTAGTTCTTT TACTTGGGGA 
AATAACATAA ATCCAAATAA TTCGTCAGGA TGGGATGAAT CTTCTAAACC TAATCCTTCC 
CAGGGATGGG GAGATCCTCC AAAGTCTAAT CAGTCTCTAG GTTGGGGAGA TTCGTCAAAG 
CCAGTCACTT CTCCAGACTG GAACAAGCAA CAAGACATTG TCGGGTCTTG GGGAATCCCA 
CCAGCAACAG GCAAACCTCC TGGTACAGGC TGGCTGGGAG GACCTATACC AGCCCCAACA 
AAAGAAGAAG AGCCCACAGG CTGGGAGGAA CCATCCCCAG AATCCATACG TCGCAAAATG 
GAGATCGATG ACGGAACTTC AGCTTGGGGA GATCCGAGCA AATACAACTA CAAAAATGTG 
AACATGTGGA ACAAAAATGT CCCAAATGGC AGCAGCCGTT CAGACCAGCA AGCACAGGTA 
CACCAGTTGC TACCATCTGC AAGTGCCATC TCAAACAAAG AGGCAGGCAG TGGCTCTGGC 
TGGGGTGAGC CCTGGGGGGA GCCTTCTACT CCAGCCACAA CTGTGGATAA TGGTACTTCA 
GCATGGGGTA AACCCATAGA CAGTGGTCCC AGCTGGGGGG AGCCCATTGC TGCAGCATCC 
AACACATCCA CGTGGGGCTC CAGCTCTGTT GGTCCTCAAG CATTAAGCAA ATCTGGGCCA 
AAATCTATGC AAGATGGCTG GTGTGGTGAT GATATGCCAT TGCCTGGAAA TCGCCCCACT 
GGCTGGGAAG AGGAAGAGGA TGTAGAGATT GGAATGTGGA ATAGTAATTC ATCTCAAGAG 
CTTAACTCAT CTTTAAATTG GCCTCCATAT ACAAAGAAAA TGTCATCGAA GGGTCTGAGT 
GGCAAAAAAA GGAGAAGGGA AAGGGGAACC ATGAAAGGTG GAAACAAACA AGAAGAAGCA 
TGGATAAATC CATTCGTTAA ACAGTTTTCA AATATCAGTT TTTCGAGAGA CTCACCCGAA 
GAAAATGTAC AGAGCAATAA GATGGATCTT TCCGGAGGAA TGTTACAGGA CAAACGAATG 
GAGATAGATA AACATAGCCT AAATATTGGT GATTACAATC GAACGGTCGG GAAAGGCCCT 
GGTTCTCGGC CTCAGATTTC CAAAGAGTCT TCCATGGAGC GCAGCCCTTA CTTTGATAAG 
GATGGCATTG TAGCAGATGA ATCCCAAAAC ATGCAGTTTA TGTCCAGTCA AAGCATGAAG 
CTTCCCCCTT CAAATAGTGC ACTACCTAAC CAGGCCCTTG GCTCCATAGC AGGGCTGGGT 
ATGCAAAACT TGAATTCTGT TAGACAGAAT GGCAATCCCA GTATGTTTGG TGTTGGAAAC 
ACAGCAGCAC AACCCCGGGG CATGCAGCAG CCTCCAGCAC AACCTCTTAG TTCATCTCAG 
CCTAATCTTC GTGCTCAAGT GCCTCCTCCA TTACTCTCCC CTCAGGTTCC AGTTTCATTG 
CTGAAGTATG CACCAAACAA CGGTGGCCTG AATCCGCTCT TTGGCCCTCA ACAGGTAGCC 
ATGCTGAACC AGCTATCCCA ACTAAACCAG CTTTCTCAGA TCTCCCAGTT ACAGAGATTG 
TTAGCGCAGC AGCAAAGGGC GCAGAGTCAG AGAAGCGTGC CTTCTGGGAA CCGGCAGCAG 
CAAGACCAGC AGGGTCGACC TCTTAGCGTG CAGCAGCAGA TGATGCAGCA GTCCCGGCAA 
CTCGATCCCA GCCTGTTGGT GAAGCAGCAG ACCCCGCCAT CTCAGCAGCA GCCACTCCAT 
CAGCCAGCCA TGAAATCCTT CCTCGAGAAT GTCATGCCCC ACACTACACC TGAGCTGCAG 
AAAGGGCCAT CACCAATAAA TGCTTTCAGC AACTTCCCGA TAGGCTTGAA CTCAAACTTG 
AATGTAAATA TGGATATGAA CAGTATTAAA GAGCCACAGT CAAGACTAAG GAAGTGGACT 
ACAGTGGACA GCATTTCTGT GAACACATCT TTGGATCAAA ACTCCAGCAA ACATGGTGCT 
ATTTCAAGTG GTTTCAGGCT GGAAGAGTCT CCATTTGTTC CCTATGACTT TATGAACAGC 
AGTACTTCTC CAGCCAGCCC TCCGGGTTCA ATAGGAGATG GCTGGCCACG TGCCAAATCG 
CCTAACGGCT CTAGCAGTGT TAACTGGCCA CCAGAATTTC GCCCTGGTGA ACCATGGAAA 
GGTTATCCAA ACATTGACCC TGAAACTGAC CCTTACGTCA CTCCTGGCAG TGTCATAAAC 
AATCTTTCAA TTAATACTGT GCGGGAAGTT GACCACCTCA GGGACAGGAA CAGTGGGTCA 
TCCTCATCCT TGAACACCAC GCTGCCTTCA ACTAGTGCCT GGTCATCCAT TCGTGCCTCC 
AACTACAACG TTCCCCTCAG CAGTACAGCA CAAAGCACTT CAGCCAGAAA TAGTGATTCC 
AAATTGACAT GGTCTCCTGG TTCAGTTACA AACACCTCTC TGGCTCATGA GCTGTGGAAG 
GTCCCTTTAC CACCTAAAAA CATCACTGCT CCGTCCCGCC CACCTCCGGG ACTCACTGGT 
CAGAAGCCAC CCTTGTCTAC GTGGGATAAC TCTCCCCTTC GTGTAGGTGG AGGATGGGGA 
AATTCTGACG CCAGGTACAC CCCAGGTTCC AGTTGGGGTG AGAGCAGCTC AGGGAGAATA 
ACAAATTGGC TTGTTCTAAA GAACCTCACA CCTCAGAACG TGCACCGAGC CAAGCGAAAT 
AGATCCCCAG TCTGGCTCCA GAGCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022275647.1
CDS	280..5769
Translation	MQLVAEPGLEARCPGSQDIPLPEEWNRELEAKATKDVERTLSRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNPQQQQQPQPPQQPQQPPQQPPQPPQQQPQPAQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLSSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSPSDSSTNCKNAVVNDSPETEAWPSVPGSDPELASECMDADSASSSESERNITIMASGSTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSPGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLDNNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVGGSSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVMNGTSLSHLSNGEPKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGINGPMGTNFQVNTNKGGGVWESGATNSQNASWGSGNGANSGGSRRGWGTPAQNTGTNIPSVEWSKLPSNQHSNDSANGNGKKFTNGWKSTEEEDQASAASQTTEQNSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACTDKTSPTSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDAAATGMVKSNQWGNSKEEKSAWNDSQKSKQGWGDGQKSNQGWSVSASDNWGETSRSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVTSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPTKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSASAISNKEAGSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASNTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGTMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERSPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPSLLVKQQTPPSQQQPLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQNVHRAKRNRSPVWLQSL

Target ORF information:

RefSeq Version	XM_022419939.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022419939.1

ORF Insert Sequence:

ATGCAACTAG TGGCAGAGCC AGGCCTAGAA GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG AGAATTGGAA GCTAAAGCTA CCAAAGACGT AGAAAGGACT 
CTTAGCAGGG ATTTAGTGCA AGAAGAAGAA CAGTTGATGG AAGAAAAGAA AAAGAAAAAA 
GACGACAAGA AAAAGAAGGA AGCTGCTCAA AAGAAGGCCA CTGAACAAAA AATCAAAGTG 
CCAGAACAGA TAAAGCCCAG TGTAAGCCAG CCTCAGCCTG CCAACTCTAA TAACGGCACT 
TCCACAGCAA CCAGCACTAA TAATAATGCC AAGCGAGCCA CAGCCAACAA TCCGCAGCAG 
CAGCAGCAGC CACAGCCGCC ACAGCAGCCA CAGCAGCCGC CGCAGCAGCC ACCGCAGCCG 
CCACAGCAGC AGCCGCAGCC AGCACAGGCC TTGCCTCGGT ATCCTCGGGA AGTACCACCA 
CGATTTCGCC ACCAGGAACA CAAACAGCTT CTAAAGAGGG GTCAGCATTT TCCTGTCATA 
GCAGCAAACC TGGGATCTGC TGTTAAAGTG TTAAGCAGCC AGTCAGAAAG CAGTGCTTTA 
ACAAATCAAC AGCCACAAAA TAACGGAGAG GTGCAGAACA GCAAAAACCA GTCAGATATA 
AACCACAGTA CTTCAGGATC CCATTATGAA AATTCACAGC GGGGACCTGT GTCTTCTCCG 
AGTGACTCTA GCACAAATTG TAAGAATGCT GTTGTAAACG ACTCCCCTGA GACAGAAGCA 
TGGCCCTCAG TCCCTGGCAG TGATCCAGAG TTGGCTTCAG AATGTATGGA TGCTGATTCT 
GCCTCCAGTT CTGAATCAGA GAGAAACATC ACTATCATGG CTTCAGGGAG CACAGGTGGT 
GAAAAAGATG GCCTTCGGAA TAGCACTGGA CTTGGTTCCC AAAACAAGTT TGTGGTTGGT 
AGCAGCAGCA ATAATGTGGG CCATGGAAGT AGTCCTGGGC CATGGGGTTT TTCCCATGGA 
GCCATAATAA GCACATGTCA GGTCTCTGTG GATGCTCCTG AAAGCAAATC AGAAAGTAGC 
AACAATAGAA TGAATGCTTG GGGCACTGTA AGTTCTTCAT CAAATGGAGG GTTAAATCCA 
AGCACTTTGA ATTCAGCTAG CAACCATGGT GCCTGGCCAG TATTAGACAA CAACGGACTT 
GCCCTCAAAG GGCCTGTAGG GAGCGGTAGT TCTGGCATCA ATATTCAGTG TAGTACCATA 
GGCCAGATGC CTAACAATCA GAGTATTAAC TCTAAAGTGG GTGGTTCTTC CACCCATGGT 
ACCTGGGGAA GCCTTCAGGA AACTTGTGAA TCTGAAGTAA GTGGTACACA GAAGGTTTCA 
TTCAGTGGTC AACCTCAGAA TATTACCACT GAGATGACTG GACCAAATAA CACTACTAAC 
TTTATGACCT CTAGTTTACC AAACTCCGGT TCAGTACAGA ATAACGAACT GCCTAGTAAT 
ACAGGGGCCT GGCGTGTGAG CACAATGAAT CATCCTCAGA TACAGGCTCC ATCGGTTATG 
AATGGCACTT CCCTTTCTCA TCTTAGCAAT GGAGAGCCAA AAAGTGGAGG CTCTTACGGT 
ACTACGTGGG GTGCCTATGG TTCTAATTAC TCTGGAGACA AATGTTCGAG CCCTAACGGC 
CAAGCTAATG GTGACACTGT GAATGCAACT CTAATGCAGC CTGGCATAAA TGGGCCTATG 
GGCACTAACT TTCAAGTGAA CACAAATAAA GGAGGAGGTG TGTGGGAGTC TGGGGCAACG 
AATTCCCAGA ATGCATCGTG GGGAAGTGGA AATGGCGCAA ATTCTGGAGG AAGTCGACGA 
GGATGGGGAA CTCCTGCACA AAACACTGGC ACTAATATAC CCAGCGTGGA GTGGAGCAAA 
CTGCCTAGCA ATCAGCATTC CAATGACAGT GCAAATGGCA ATGGTAAGAA GTTTACAAAT 
GGATGGAAAT CTACTGAGGA AGAGGATCAG GCTTCTGCCG CATCTCAGAC AACTGAGCAG 
AACAGTGTGT GGGCCAAAAC AGGAGGTACA GTGGAGAGTG AAGGTAGTAC AGAAAGCACT 
GGACGCCTCG AGGAGAAAGC CACTGGGGAA AATCAGAGTA GAGATCGGAG AAAAATTGAT 
CAGCACACAT TACTCCAAAG CATCGTAAAC AGAACTGACT TGGATCCACG TGTCCTCTCC 
AACTCTGGTT GGGGACAGAC TCCTATTAAG CAGAATACTG CCTGGGATAC GGAAACATCA 
CCTAGAGGGG AAAGAAAGAC TGACAATGGG ACAGAGGCCT GGGGAAGCTC TGCAACACAG 
ACTTTTAACT CAGGGGCATG TACAGATAAG ACTAGCCCTA CTAGTAATGA TACCTCATCT 
GTATCGGGGT GGGGAGATCC CAAACCTGCT CTGAGGTGGG GAGATTCCAA AGGCTCAAGC 
TGCCAGGGGG GATGGGAAGA TGATGCTGCT GCTACAGGAA TGGTCAAGAG CAATCAGTGG 
GGGAACAGCA AAGAAGAGAA GTCCGCATGG AATGATTCGC AAAAGAGCAA ACAGGGATGG 
GGTGATGGAC AGAAATCAAA CCAAGGGTGG TCCGTTTCTG CCAGTGATAA CTGGGGAGAA 
ACGTCAAGGA GTAACCATTG GGGTGAGGCT AATAAGAAAT CTAGCTCAGG AGGTAGTGAC 
AGTGACAGGT CTGTTTCTGG TTGGAATGAA CTTGGTAAAA CTAGTTCTTT TACTTGGGGA 
AATAACATAA ATCCAAATAA TTCGTCAGGA TGGGATGAAT CTTCTAAACC TAATCCTTCC 
CAGGGATGGG GAGATCCTCC AAAGTCTAAT CAGTCTCTAG GTTGGGGAGA TTCGTCAAAG 
CCAGTCACTT CTCCAGACTG GAACAAGCAA CAAGACATTG TCGGGTCTTG GGGAATCCCA 
CCAGCAACAG GCAAACCTCC TGGTACAGGC TGGCTGGGAG GACCTATACC AGCCCCAACA 
AAAGAAGAAG AGCCCACAGG CTGGGAGGAA CCATCCCCAG AATCCATACG TCGCAAAATG 
GAGATCGATG ACGGAACTTC AGCTTGGGGA GATCCGAGCA AATACAACTA CAAAAATGTG 
AACATGTGGA ACAAAAATGT CCCAAATGGC AGCAGCCGTT CAGACCAGCA AGCACAGGTA 
CACCAGTTGC TACCATCTGC AAGTGCCATC TCAAACAAAG AGGCAGGCAG TGGCTCTGGC 
TGGGGTGAGC CCTGGGGGGA GCCTTCTACT CCAGCCACAA CTGTGGATAA TGGTACTTCA 
GCATGGGGTA AACCCATAGA CAGTGGTCCC AGCTGGGGGG AGCCCATTGC TGCAGCATCC 
AACACATCCA CGTGGGGCTC CAGCTCTGTT GGTCCTCAAG CATTAAGCAA ATCTGGGCCA 
AAATCTATGC AAGATGGCTG GTGTGGTGAT GATATGCCAT TGCCTGGAAA TCGCCCCACT 
GGCTGGGAAG AGGAAGAGGA TGTAGAGATT GGAATGTGGA ATAGTAATTC ATCTCAAGAG 
CTTAACTCAT CTTTAAATTG GCCTCCATAT ACAAAGAAAA TGTCATCGAA GGGTCTGAGT 
GGCAAAAAAA GGAGAAGGGA AAGGGGAACC ATGAAAGGTG GAAACAAACA AGAAGAAGCA 
TGGATAAATC CATTCGTTAA ACAGTTTTCA AATATCAGTT TTTCGAGAGA CTCACCCGAA 
GAAAATGTAC AGAGCAATAA GATGGATCTT TCCGGAGGAA TGTTACAGGA CAAACGAATG 
GAGATAGATA AACATAGCCT AAATATTGGT GATTACAATC GAACGGTCGG GAAAGGCCCT 
GGTTCTCGGC CTCAGATTTC CAAAGAGTCT TCCATGGAGC GCAGCCCTTA CTTTGATAAG 
GATGGCATTG TAGCAGATGA ATCCCAAAAC ATGCAGTTTA TGTCCAGTCA AAGCATGAAG 
CTTCCCCCTT CAAATAGTGC ACTACCTAAC CAGGCCCTTG GCTCCATAGC AGGGCTGGGT 
ATGCAAAACT TGAATTCTGT TAGACAGAAT GGCAATCCCA GTATGTTTGG TGTTGGAAAC 
ACAGCAGCAC AACCCCGGGG CATGCAGCAG CCTCCAGCAC AACCTCTTAG TTCATCTCAG 
CCTAATCTTC GTGCTCAAGT GCCTCCTCCA TTACTCTCCC CTCAGGTTCC AGTTTCATTG 
CTGAAGTATG CACCAAACAA CGGTGGCCTG AATCCGCTCT TTGGCCCTCA ACAGGTAGCC 
ATGCTGAACC AGCTATCCCA ACTAAACCAG CTTTCTCAGA TCTCCCAGTT ACAGAGATTG 
TTAGCGCAGC AGCAAAGGGC GCAGAGTCAG AGAAGCGTGC CTTCTGGGAA CCGGCAGCAG 
CAAGACCAGC AGGGTCGACC TCTTAGCGTG CAGCAGCAGA TGATGCAGCA GTCCCGGCAA 
CTCGATCCCA GCCTGTTGGT GAAGCAGCAG ACCCCGCCAT CTCAGCAGCA GCCACTCCAT 
CAGCCAGCCA TGAAATCCTT CCTCGAGAAT GTCATGCCCC ACACTACACC TGAGCTGCAG 
AAAGGGCCAT CACCAATAAA TGCTTTCAGC AACTTCCCGA TAGGCTTGAA CTCAAACTTG 
AATGTAAATA TGGATATGAA CAGTATTAAA GAGCCACAGT CAAGACTAAG GAAGTGGACT 
ACAGTGGACA GCATTTCTGT GAACACATCT TTGGATCAAA ACTCCAGCAA ACATGGTGCT 
ATTTCAAGTG GTTTCAGGCT GGAAGAGTCT CCATTTGTTC CCTATGACTT TATGAACAGC 
AGTACTTCTC CAGCCAGCCC TCCGGGTTCA ATAGGAGATG GCTGGCCACG TGCCAAATCG 
CCTAACGGCT CTAGCAGTGT TAACTGGCCA CCAGAATTTC GCCCTGGTGA ACCATGGAAA 
GGTTATCCAA ACATTGACCC TGAAACTGAC CCTTACGTCA CTCCTGGCAG TGTCATAAAC 
AATCTTTCAA TTAATACTGT GCGGGAAGTT GACCACCTCA GGGACAGGAA CAGTGGGTCA 
TCCTCATCCT TGAACACCAC GCTGCCTTCA ACTAGTGCCT GGTCATCCAT TCGTGCCTCC 
AACTACAACG TTCCCCTCAG CAGTACAGCA CAAAGCACTT CAGCCAGAAA TAGTGATTCC 
AAATTGACAT GGTCTCCTGG TTCAGTTACA AACACCTCTC TGGCTCATGA GCTGTGGAAG 
GTCCCTTTAC CACCTAAAAA CATCACTGCT CCGTCCCGCC CACCTCCGGG ACTCACTGGT 
CAGAAGCCAC CCTTGTCTAC GTGGGATAAC TCTCCCCTTC GTGTAGGTGG AGGATGGGGA 
AATTCTGACG CCAGGTACAC CCCAGGTTCC AGTTGGGGTG AGAGCAGCTC AGGGAGAATA 
ACAAATTGGC TTGTTCTAAA GAACCTCACA CCTCAGAACG TGCACCGAGC CAAGCGAAAT 
AGATCCCCAG TCTGGCTCCA GAGCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_022419941.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022275649.1 Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X13, mRNA.

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CloneID	OCe288919
Clone ID Related Accession (Same CDS sequence)	XM_022419941.1
Accession Version	XM_022419941.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	trinucleotide repeat-containing gene 6A protein isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACTAG TGGCAGAGCC AGGCCTAGAA GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG AGAATTGGAA GCTAAAGCTA CCAAAGACGT AGAAAGGACT 
CTTAGCAGGG ATTTAGTGCA AGAAGAAGAA CAGTTGATGG AAGAAAAGAA AAAGAAAAAA 
GACGACAAGA AAAAGAAGGA AGCTGCTCAA AAGAAGGCCA CTGAACAAAA AATCAAAGTG 
CCAGAACAGA TAAAGCCCAG TGTAAGCCAG CCTCAGCCTG CCAACTCTAA TAACGGCACT 
TCCACAGCAA CCAGCACTAA TAATAATGCC AAGCGAGCCA CAGCCAACAA TCCGCAGCAG 
CAGCAGCAGC CACAGCCGCC ACAGCAGCCA CAGCAGCCGC CGCAGCAGCC ACCGCAGCCG 
CCACAGCAGC AGCCGCAGCC AGCACAGGCC TTGCCTCGGT ATCCTCGGGA AGTACCACCA 
CGATTTCGCC ACCAGGAACA CAAACAGCTT CTAAAGAGGG GTCAGCATTT TCCTGTCATA 
GCAGCAAACC TGGGATCTGC TGTTAAAGTG TTAAGCAGCC AGTCAGAAAG CAGTGCTTTA 
ACAAATCAAC AGCCACAAAA TAACGGAGAG GTGCAGAACA GCAAAAACCA GTCAGATATA 
AACCACAGTA CTTCAGGATC CCATTATGAA AATTCACAGC GGGGACCTGT GTCTTCTCCG 
AGTGACTCTA GCACAAATTG TAAGAATGCT GTTGTAAACG ACTCCCCTGA GACAGAAGCA 
TGGCCCTCAG TCCCTGGCAG TGATCCAGAG TTGGCTTCAG AATGTATGGA TGCTGATTCT 
GCCTCCAGTT CTGAATCAGA GAGAAACATC ACTATCATGG CTTCAGGGAG CACAGGTGGT 
GAAAAAGATG GCCTTCGGAA TAGCACTGGA CTTGGTTCCC AAAACAAGTT TGTGGTTGGT 
AGCAGCAGCA ATAATGTGGG CCATGGAAGT AGTCCTGGGC CATGGGGTTT TTCCCATGGA 
GCCATAATAA GCACATGTCA GGTCTCTGTG GATGCTCCTG AAAGCAAATC AGAAAGTAGC 
AACAATAGAA TGAATGCTTG GGGCACTGTA AGTTCTTCAT CAAATGGAGG GTTAAATCCA 
AGCACTTTGA ATTCAGCTAG CAACCATGGT GCCTGGCCAG TATTAGACAA CAACGGACTT 
GCCCTCAAAG GGCCTGTAGG GAGCGGTAGT TCTGGCATCA ATATTCAGTG TAGTACCATA 
GGCCAGATGC CTAACAATCA GAGTATTAAC TCTAAAGTGG GTGGTTCTTC CACCCATGGT 
ACCTGGGGAA GCCTTCAGGA AACTTGTGAA TCTGAAGTAA GTGGTACACA GAAGGTTTCA 
TTCAGTGGTC AACCTCAGAA TATTACCACT GAGATGACTG GACCAAATAA CACTACTAAC 
TTTATGACCT CTAGTTTACC AAACTCCGGT TCAGTACAGA ATAACGAACT GCCTAGTAAT 
ACAGGGGCCT GGCGTGTGAG CACAATGAAT CATCCTCAGA TACAGGCTCC ATCGGTTATG 
AATGGCACTT CCCTTTCTCA TCTTAGCAAT GGAGAGCCAA AAAGTGGAGG CTCTTACGGT 
ACTACGTGGG GTGCCTATGG TTCTAATTAC TCTGGAGACA AATGTTCGAG CCCTAACGGC 
CAAGCTAATG GTGACACTGT GAATGCAACT CTAATGCAGC CTGGCATAAA TGGGCCTATG 
GGCACTAACT TTCAAGTGAA CACAAATAAA GGAGGAGGTG TGTGGGAGTC TGGGGCAACG 
AATTCCCAGA ATGCATCGTG GGGAAGTGGA AATGGCGCAA ATTCTGGAGG AAGTCGACGA 
GGATGGGGAA CTCCTGCACA AAACACTGGC ACTAATATAC CCAGCGTGGA GTGGAGCAAA 
CTGCCTAGCA ATCAGCATTC CAATGACAGT GCAAATGGCA ATGGTAAGAA GTTTACAAAT 
GGATGGAAAT CTACTGAGGA AGAGGATCAG GCTTCTGCCG CATCTCAGAC AACTGAGCAG 
AACAGTGTGT GGGCCAAAAC AGGAGGTACA GTGGAGAGTG AAGGTAGTAC AGAAAGCACT 
GGACGCCTCG AGGAGAAAGC CACTGGGGAA AATCAGAGTA GAGATCGGAG AAAAATTGAT 
CAGCACACAT TACTCCAAAG CATCGTAAAC AGAACTGACT TGGATCCACG TGTCCTCTCC 
AACTCTGGTT GGGGACAGAC TCCTATTAAG CAGAATACTG CCTGGGATAC GGAAACATCA 
CCTAGAGGGG AAAGAAAGAC TGACAATGGG ACAGAGGCCT GGGGAAGCTC TGCAACACAG 
ACTTTTAACT CAGGGGCATG TACAGATAAG ACTAGCCCTA CTAGTAATGA TACCTCATCT 
GTATCGGGGT GGGGAGATCC CAAACCTGCT CTGAGGTGGG GAGATTCCAA AGGCTCAAGC 
TGCCAGGGGG GATGGGAAGA TGATGCTGCT GCTACAGGAA TGGTCAAGAG CAATCAGTGG 
GGGAACAGCA AAGAAGAGAA GTCCGCATGG AATGATTCGC AAAAGAGCAA ACAGGGATGG 
GGTGATGGAC AGAAATCAAA CCAAGGGTGG TCCGTTTCTG CCAGTGATAA CTGGGGAGAA 
ACGTCAAGGA GTAACCATTG GGGTGAGGCT AATAAGAAAT CTAGCTCAGG AGGTAGTGAC 
AGTGACAGGT CTGTTTCTGG TTGGAATGAA CTTGGTAAAA CTAGTTCTTT TACTTGGGGA 
AATAACATAA ATCCAAATAA TTCGTCAGGA TGGGATGAAT CTTCTAAACC TAATCCTTCC 
CAGGGATGGG GAGATCCTCC AAAGTCTAAT CAGTCTCTAG GTTGGGGAGA TTCGTCAAAG 
CCAGTCACTT CTCCAGACTG GAACAAGCAA CAAGACATTG TCGGGTCTTG GGGAATCCCA 
CCAGCAACAG GCAAACCTCC TGGTACAGGC TGGCTGGGAG GACCTATACC AGCCCCAACA 
AAAGAAGAAG AGCCCACAGG CTGGGAGGAA CCATCCCCAG AATCCATACG TCGCAAAATG 
GAGATCGATG ACGGAACTTC AGCTTGGGGA GATCCGAGCA AATACAACTA CAAAAATGTG 
AACATGTGGA ACAAAAATGT CCCAAATGGC AGCAGCCGTT CAGACCAGCA AGCACAGGTA 
CACCAGTTGC TACCATCTGC AAGTGCCATC TCAAACAAAG AGGCAGGCAG TGGCTCTGGC 
TGGGGTGAGC CCTGGGGGGA GCCTTCTACT CCAGCCACAA CTGTGGATAA TGGTACTTCA 
GCATGGGGTA AACCCATAGA CAGTGGTCCC AGCTGGGGGG AGCCCATTGC TGCAGCATCC 
AACACATCCA CGTGGGGCTC CAGCTCTGTT GGTCCTCAAG CATTAAGCAA ATCTGGGCCA 
AAATCTATGC AAGATGGCTG GTGTGGTGAT GATATGCCAT TGCCTGGAAA TCGCCCCACT 
GGCTGGGAAG AGGAAGAGGA TGTAGAGATT GGAATGTGGA ATAGTAATTC ATCTCAAGAG 
CTTAACTCAT CTTTAAATTG GCCTCCATAT ACAAAGAAAA TGTCATCGAA GGGTCTGAGT 
GGCAAAAAAA GGAGAAGGGA AAGGGGAACC ATGAAAGGTG GAAACAAACA AGAAGAAGCA 
TGGATAAATC CATTCGTTAA ACAGTTTTCA AATATCAGTT TTTCGAGAGA CTCACCCGAA 
GAAAATGTAC AGAGCAATAA GATGGATCTT TCCGGAGGAA TGTTACAGGA CAAACGAATG 
GAGATAGATA AACATAGCCT AAATATTGGT GATTACAATC GAACGGTCGG GAAAGGCCCT 
GGTTCTCGGC CTCAGATTTC CAAAGAGTCT TCCATGGAGC GCAGCCCTTA CTTTGATAAG 
GATGGCATTG TAGCAGATGA ATCCCAAAAC ATGCAGTTTA TGTCCAGTCA AAGCATGAAG 
CTTCCCCCTT CAAATAGTGC ACTACCTAAC CAGGCCCTTG GCTCCATAGC AGGGCTGGGT 
ATGCAAAACT TGAATTCTGT TAGACAGAAT GGCAATCCCA GTATGTTTGG TGTTGGAAAC 
ACAGCAGCAC AACCCCGGGG CATGCAGCAG CCTCCAGCAC AACCTCTTAG TTCATCTCAG 
CCTAATCTTC GTGCTCAAGT GCCTCCTCCA TTACTCTCCC CTCAGGTTCC AGTTTCATTG 
CTGAAGTATG CACCAAACAA CGGTGGCCTG AATCCGCTCT TTGGCCCTCA ACAGGTAGCC 
ATGCTGAACC AGCTATCCCA ACTAAACCAG CTTTCTCAGA TCTCCCAGTT ACAGAGATTG 
TTAGCGCAGC AGCAAAGGGC GCAGAGTCAG AGAAGCGTGC CTTCTGGGAA CCGGCAGCAG 
CAAGACCAGC AGGGTCGACC TCTTAGCGTG CAGCAGCAGA TGATGCAGCA GTCCCGGCAA 
CTCGATCCCA GCCTGTTGGT GAAGCAGCAG ACCCCGCCAT CTCAGCAGCA GCCACTCCAT 
CAGCCAGCCA TGAAATCCTT CCTCGAGAAT GTCATGCCCC ACACTACACC TGAGCTGCAG 
AAAGGGCCAT CACCAATAAA TGCTTTCAGC AACTTCCCGA TAGGCTTGAA CTCAAACTTG 
AATGTAAATA TGGATATGAA CAGTATTAAA GAGCCACAGT CAAGACTAAG GAAGTGGACT 
ACAGTGGACA GCATTTCTGT GAACACATCT TTGGATCAAA ACTCCAGCAA ACATGGTGCT 
ATTTCAAGTG GTTTCAGGCT GGAAGAGTCT CCATTTGTTC CCTATGACTT TATGAACAGC 
AGTACTTCTC CAGCCAGCCC TCCGGGTTCA ATAGGAGATG GCTGGCCACG TGCCAAATCG 
CCTAACGGCT CTAGCAGTGT TAACTGGCCA CCAGAATTTC GCCCTGGTGA ACCATGGAAA 
GGTTATCCAA ACATTGACCC TGAAACTGAC CCTTACGTCA CTCCTGGCAG TGTCATAAAC 
AATCTTTCAA TTAATACTGT GCGGGAAGTT GACCACCTCA GGGACAGGAA CAGTGGGTCA 
TCCTCATCCT TGAACACCAC GCTGCCTTCA ACTAGTGCCT GGTCATCCAT TCGTGCCTCC 
AACTACAACG TTCCCCTCAG CAGTACAGCA CAAAGCACTT CAGGTTCCAG TTGGGGTGAG 
AGCAGCTCAG GGAGAATAAC AAATTGGCTT GTTCTAAAGA ACCTCACACC TCAGAACGTG 
CACCGAGCCA AGCGAAATAG ATCCCCAGTC TGGCTCCAGA GCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022275649.1
CDS	282..5549
Translation	MQLVAEPGLEARCPGSQDIPLPEEWNRELEAKATKDVERTLSRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNPQQQQQPQPPQQPQQPPQQPPQPPQQQPQPAQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLSSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSPSDSSTNCKNAVVNDSPETEAWPSVPGSDPELASECMDADSASSSESERNITIMASGSTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSPGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLDNNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVGGSSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVMNGTSLSHLSNGEPKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGINGPMGTNFQVNTNKGGGVWESGATNSQNASWGSGNGANSGGSRRGWGTPAQNTGTNIPSVEWSKLPSNQHSNDSANGNGKKFTNGWKSTEEEDQASAASQTTEQNSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACTDKTSPTSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDAAATGMVKSNQWGNSKEEKSAWNDSQKSKQGWGDGQKSNQGWSVSASDNWGETSRSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVTSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPTKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSASAISNKEAGSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASNTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGTMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERSPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPSLLVKQQTPPSQQQPLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSGSSWGESSSGRITNWLVLKNLTPQNVHRAKRNRSPVWLQSL

Target ORF information:

RefSeq Version	XM_022419941.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022419941.1

ORF Insert Sequence:

ATGCAACTAG TGGCAGAGCC AGGCCTAGAA GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG AGAATTGGAA GCTAAAGCTA CCAAAGACGT AGAAAGGACT 
CTTAGCAGGG ATTTAGTGCA AGAAGAAGAA CAGTTGATGG AAGAAAAGAA AAAGAAAAAA 
GACGACAAGA AAAAGAAGGA AGCTGCTCAA AAGAAGGCCA CTGAACAAAA AATCAAAGTG 
CCAGAACAGA TAAAGCCCAG TGTAAGCCAG CCTCAGCCTG CCAACTCTAA TAACGGCACT 
TCCACAGCAA CCAGCACTAA TAATAATGCC AAGCGAGCCA CAGCCAACAA TCCGCAGCAG 
CAGCAGCAGC CACAGCCGCC ACAGCAGCCA CAGCAGCCGC CGCAGCAGCC ACCGCAGCCG 
CCACAGCAGC AGCCGCAGCC AGCACAGGCC TTGCCTCGGT ATCCTCGGGA AGTACCACCA 
CGATTTCGCC ACCAGGAACA CAAACAGCTT CTAAAGAGGG GTCAGCATTT TCCTGTCATA 
GCAGCAAACC TGGGATCTGC TGTTAAAGTG TTAAGCAGCC AGTCAGAAAG CAGTGCTTTA 
ACAAATCAAC AGCCACAAAA TAACGGAGAG GTGCAGAACA GCAAAAACCA GTCAGATATA 
AACCACAGTA CTTCAGGATC CCATTATGAA AATTCACAGC GGGGACCTGT GTCTTCTCCG 
AGTGACTCTA GCACAAATTG TAAGAATGCT GTTGTAAACG ACTCCCCTGA GACAGAAGCA 
TGGCCCTCAG TCCCTGGCAG TGATCCAGAG TTGGCTTCAG AATGTATGGA TGCTGATTCT 
GCCTCCAGTT CTGAATCAGA GAGAAACATC ACTATCATGG CTTCAGGGAG CACAGGTGGT 
GAAAAAGATG GCCTTCGGAA TAGCACTGGA CTTGGTTCCC AAAACAAGTT TGTGGTTGGT 
AGCAGCAGCA ATAATGTGGG CCATGGAAGT AGTCCTGGGC CATGGGGTTT TTCCCATGGA 
GCCATAATAA GCACATGTCA GGTCTCTGTG GATGCTCCTG AAAGCAAATC AGAAAGTAGC 
AACAATAGAA TGAATGCTTG GGGCACTGTA AGTTCTTCAT CAAATGGAGG GTTAAATCCA 
AGCACTTTGA ATTCAGCTAG CAACCATGGT GCCTGGCCAG TATTAGACAA CAACGGACTT 
GCCCTCAAAG GGCCTGTAGG GAGCGGTAGT TCTGGCATCA ATATTCAGTG TAGTACCATA 
GGCCAGATGC CTAACAATCA GAGTATTAAC TCTAAAGTGG GTGGTTCTTC CACCCATGGT 
ACCTGGGGAA GCCTTCAGGA AACTTGTGAA TCTGAAGTAA GTGGTACACA GAAGGTTTCA 
TTCAGTGGTC AACCTCAGAA TATTACCACT GAGATGACTG GACCAAATAA CACTACTAAC 
TTTATGACCT CTAGTTTACC AAACTCCGGT TCAGTACAGA ATAACGAACT GCCTAGTAAT 
ACAGGGGCCT GGCGTGTGAG CACAATGAAT CATCCTCAGA TACAGGCTCC ATCGGTTATG 
AATGGCACTT CCCTTTCTCA TCTTAGCAAT GGAGAGCCAA AAAGTGGAGG CTCTTACGGT 
ACTACGTGGG GTGCCTATGG TTCTAATTAC TCTGGAGACA AATGTTCGAG CCCTAACGGC 
CAAGCTAATG GTGACACTGT GAATGCAACT CTAATGCAGC CTGGCATAAA TGGGCCTATG 
GGCACTAACT TTCAAGTGAA CACAAATAAA GGAGGAGGTG TGTGGGAGTC TGGGGCAACG 
AATTCCCAGA ATGCATCGTG GGGAAGTGGA AATGGCGCAA ATTCTGGAGG AAGTCGACGA 
GGATGGGGAA CTCCTGCACA AAACACTGGC ACTAATATAC CCAGCGTGGA GTGGAGCAAA 
CTGCCTAGCA ATCAGCATTC CAATGACAGT GCAAATGGCA ATGGTAAGAA GTTTACAAAT 
GGATGGAAAT CTACTGAGGA AGAGGATCAG GCTTCTGCCG CATCTCAGAC AACTGAGCAG 
AACAGTGTGT GGGCCAAAAC AGGAGGTACA GTGGAGAGTG AAGGTAGTAC AGAAAGCACT 
GGACGCCTCG AGGAGAAAGC CACTGGGGAA AATCAGAGTA GAGATCGGAG AAAAATTGAT 
CAGCACACAT TACTCCAAAG CATCGTAAAC AGAACTGACT TGGATCCACG TGTCCTCTCC 
AACTCTGGTT GGGGACAGAC TCCTATTAAG CAGAATACTG CCTGGGATAC GGAAACATCA 
CCTAGAGGGG AAAGAAAGAC TGACAATGGG ACAGAGGCCT GGGGAAGCTC TGCAACACAG 
ACTTTTAACT CAGGGGCATG TACAGATAAG ACTAGCCCTA CTAGTAATGA TACCTCATCT 
GTATCGGGGT GGGGAGATCC CAAACCTGCT CTGAGGTGGG GAGATTCCAA AGGCTCAAGC 
TGCCAGGGGG GATGGGAAGA TGATGCTGCT GCTACAGGAA TGGTCAAGAG CAATCAGTGG 
GGGAACAGCA AAGAAGAGAA GTCCGCATGG AATGATTCGC AAAAGAGCAA ACAGGGATGG 
GGTGATGGAC AGAAATCAAA CCAAGGGTGG TCCGTTTCTG CCAGTGATAA CTGGGGAGAA 
ACGTCAAGGA GTAACCATTG GGGTGAGGCT AATAAGAAAT CTAGCTCAGG AGGTAGTGAC 
AGTGACAGGT CTGTTTCTGG TTGGAATGAA CTTGGTAAAA CTAGTTCTTT TACTTGGGGA 
AATAACATAA ATCCAAATAA TTCGTCAGGA TGGGATGAAT CTTCTAAACC TAATCCTTCC 
CAGGGATGGG GAGATCCTCC AAAGTCTAAT CAGTCTCTAG GTTGGGGAGA TTCGTCAAAG 
CCAGTCACTT CTCCAGACTG GAACAAGCAA CAAGACATTG TCGGGTCTTG GGGAATCCCA 
CCAGCAACAG GCAAACCTCC TGGTACAGGC TGGCTGGGAG GACCTATACC AGCCCCAACA 
AAAGAAGAAG AGCCCACAGG CTGGGAGGAA CCATCCCCAG AATCCATACG TCGCAAAATG 
GAGATCGATG ACGGAACTTC AGCTTGGGGA GATCCGAGCA AATACAACTA CAAAAATGTG 
AACATGTGGA ACAAAAATGT CCCAAATGGC AGCAGCCGTT CAGACCAGCA AGCACAGGTA 
CACCAGTTGC TACCATCTGC AAGTGCCATC TCAAACAAAG AGGCAGGCAG TGGCTCTGGC 
TGGGGTGAGC CCTGGGGGGA GCCTTCTACT CCAGCCACAA CTGTGGATAA TGGTACTTCA 
GCATGGGGTA AACCCATAGA CAGTGGTCCC AGCTGGGGGG AGCCCATTGC TGCAGCATCC 
AACACATCCA CGTGGGGCTC CAGCTCTGTT GGTCCTCAAG CATTAAGCAA ATCTGGGCCA 
AAATCTATGC AAGATGGCTG GTGTGGTGAT GATATGCCAT TGCCTGGAAA TCGCCCCACT 
GGCTGGGAAG AGGAAGAGGA TGTAGAGATT GGAATGTGGA ATAGTAATTC ATCTCAAGAG 
CTTAACTCAT CTTTAAATTG GCCTCCATAT ACAAAGAAAA TGTCATCGAA GGGTCTGAGT 
GGCAAAAAAA GGAGAAGGGA AAGGGGAACC ATGAAAGGTG GAAACAAACA AGAAGAAGCA 
TGGATAAATC CATTCGTTAA ACAGTTTTCA AATATCAGTT TTTCGAGAGA CTCACCCGAA 
GAAAATGTAC AGAGCAATAA GATGGATCTT TCCGGAGGAA TGTTACAGGA CAAACGAATG 
GAGATAGATA AACATAGCCT AAATATTGGT GATTACAATC GAACGGTCGG GAAAGGCCCT 
GGTTCTCGGC CTCAGATTTC CAAAGAGTCT TCCATGGAGC GCAGCCCTTA CTTTGATAAG 
GATGGCATTG TAGCAGATGA ATCCCAAAAC ATGCAGTTTA TGTCCAGTCA AAGCATGAAG 
CTTCCCCCTT CAAATAGTGC ACTACCTAAC CAGGCCCTTG GCTCCATAGC AGGGCTGGGT 
ATGCAAAACT TGAATTCTGT TAGACAGAAT GGCAATCCCA GTATGTTTGG TGTTGGAAAC 
ACAGCAGCAC AACCCCGGGG CATGCAGCAG CCTCCAGCAC AACCTCTTAG TTCATCTCAG 
CCTAATCTTC GTGCTCAAGT GCCTCCTCCA TTACTCTCCC CTCAGGTTCC AGTTTCATTG 
CTGAAGTATG CACCAAACAA CGGTGGCCTG AATCCGCTCT TTGGCCCTCA ACAGGTAGCC 
ATGCTGAACC AGCTATCCCA ACTAAACCAG CTTTCTCAGA TCTCCCAGTT ACAGAGATTG 
TTAGCGCAGC AGCAAAGGGC GCAGAGTCAG AGAAGCGTGC CTTCTGGGAA CCGGCAGCAG 
CAAGACCAGC AGGGTCGACC TCTTAGCGTG CAGCAGCAGA TGATGCAGCA GTCCCGGCAA 
CTCGATCCCA GCCTGTTGGT GAAGCAGCAG ACCCCGCCAT CTCAGCAGCA GCCACTCCAT 
CAGCCAGCCA TGAAATCCTT CCTCGAGAAT GTCATGCCCC ACACTACACC TGAGCTGCAG 
AAAGGGCCAT CACCAATAAA TGCTTTCAGC AACTTCCCGA TAGGCTTGAA CTCAAACTTG 
AATGTAAATA TGGATATGAA CAGTATTAAA GAGCCACAGT CAAGACTAAG GAAGTGGACT 
ACAGTGGACA GCATTTCTGT GAACACATCT TTGGATCAAA ACTCCAGCAA ACATGGTGCT 
ATTTCAAGTG GTTTCAGGCT GGAAGAGTCT CCATTTGTTC CCTATGACTT TATGAACAGC 
AGTACTTCTC CAGCCAGCCC TCCGGGTTCA ATAGGAGATG GCTGGCCACG TGCCAAATCG 
CCTAACGGCT CTAGCAGTGT TAACTGGCCA CCAGAATTTC GCCCTGGTGA ACCATGGAAA 
GGTTATCCAA ACATTGACCC TGAAACTGAC CCTTACGTCA CTCCTGGCAG TGTCATAAAC 
AATCTTTCAA TTAATACTGT GCGGGAAGTT GACCACCTCA GGGACAGGAA CAGTGGGTCA 
TCCTCATCCT TGAACACCAC GCTGCCTTCA ACTAGTGCCT GGTCATCCAT TCGTGCCTCC 
AACTACAACG TTCCCCTCAG CAGTACAGCA CAAAGCACTT CAGGTTCCAG TTGGGGTGAG 
AGCAGCTCAG GGAGAATAAC AAATTGGCTT GTTCTAAAGA ACCTCACACC TCAGAACGTG 
CACCGAGCCA AGCGAAATAG ATCCCCAGTC TGGCTCCAGA GCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_014114535.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013970010.1 Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X3, mRNA.

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CloneID	OCe37067
Clone ID Related Accession (Same CDS sequence)	XM_014114535.2 , XM_014114535.1
Accession Version	XM_014114535.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5904bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	trinucleotide repeat-containing gene 6A protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014114535.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACTAG TGGCAGAGCC AGGCCTAGAA GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCC AGTGTAAGCC AGCCTCAGCC TGCCAACTCT 
AATAACGGCA CTTCCACAGC AACCAGCACT AATAATAATG CCAAGCGAGC CACAGCCAAC 
AATCCGCAGC AGCAGCAGCA GCCACAGCCG CCACAGCAGC CACAGCAGCC GCCGCAGCAG 
CCACCGCAGC CGCCACAGCA GCAGCCGCAG CCAGCACAGG CCTTGCCTCG GTATCCTCGG 
GAAGTACCAC CACGATTTCG CCACCAGGAA CACAAACAGC TTCTAAAGAG GGGTCAGCAT 
TTTCCTGTCA TAGCAGCAAA CCTGGGATCT GCTGTTAAAG TGTTAAGCAG CCAGTCAGAA 
AGCAGTGCTT TAACAAATCA ACAGCCACAA AATAACGGAG AGGTGCAGAA CAGCAAAAAC 
CAGTCAGATA TAAACCACAG TACTTCAGGA TCCCATTATG AAAATTCACA GCGGGGACCT 
GTGTCTTCTC CGAGTGACTC TAGCACAAAT TGTAAGAATG CTGTTGTAAA CGACTCCCCT 
GAGACAGAAG CATGGCCCTC AGTCCCTGGC AGTGATCCAG AGTTGGCTTC AGAATGTATG 
GATGCTGATT CTGCCTCCAG TTCTGAATCA GAGAGAAACA TCACTATCAT GGCTTCAGGG 
AGCACAGGTG GTGAAAAAGA TGGCCTTCGG AATAGCACTG GACTTGGTTC CCAAAACAAG 
TTTGTGGTTG GTAGCAGCAG CAATAATGTG GGCCATGGAA GTAGTCCTGG GCCATGGGGT 
TTTTCCCATG GAGCCATAAT AAGCACATGT CAGGTCTCTG TGGATGCTCC TGAAAGCAAA 
TCAGAAAGTA GCAACAATAG AATGAATGCT TGGGGCACTG TAAGTTCTTC ATCAAATGGA 
GGGTTAAATC CAAGCACTTT GAATTCAGCT AGCAACCATG GTGCCTGGCC AGTATTAGAC 
AACAACGGAC TTGCCCTCAA AGGGCCTGTA GGGAGCGGTA GTTCTGGCAT CAATATTCAG 
TGTAGTACCA TAGGCCAGAT GCCTAACAAT CAGAGTATTA ACTCTAAAGT GGGTGGTTCT 
TCCACCCATG GTACCTGGGG AAGCCTTCAG GAAACTTGTG AATCTGAAGT AAGTGGTACA 
CAGAAGGTTT CATTCAGTGG TCAACCTCAG AATATTACCA CTGAGATGAC TGGACCAAAT 
AACACTACTA ACTTTATGAC CTCTAGTTTA CCAAACTCCG GTTCAGTACA GAATAACGAA 
CTGCCTAGTA ATACAGGGGC CTGGCGTGTG AGCACAATGA ATCATCCTCA GATACAGGCT 
CCATCGGTTA TGAATGGCAC TTCCCTTTCT CATCTTAGCA ATGGAGAGCC AAAAAGTGGA 
GGCTCTTACG GTACTACGTG GGGTGCCTAT GGTTCTAATT ACTCTGGAGA CAAATGTTCG 
AGCCCTAACG GCCAAGCTAA TGGTGACACT GTGAATGCAA CTCTAATGCA GCCTGGCATA 
AATGGGCCTA TGGGCACTAA CTTTCAAGTG AACACAAATA AAGGAGGAGG TGTGTGGGAG 
TCTGGGGCAA CGAATTCCCA GAATGCATCG TGGGGAAGTG GAAATGGCGC AAATTCTGGA 
GGAAGTCGAC GAGGATGGGG AACTCCTGCA CAAAACACTG GCACTAATAT ACCCAGCGTG 
GAGTGGAGCA AACTGCCTAG CAATCAGCAT TCCAATGACA GTGCAAATGG CAATGGTAAG 
AAGTTTACAA ATGGATGGAA ATCTACTGAG GAAGAGGATC AGGCTTCTGC CGCATCTCAG 
ACAACTGAGC AGAACAGTGT GTGGGCCAAA ACAGGAGGTA CAGTGGAGAG TGAAGGTAGT 
ACAGAAAGCA CTGGACGCCT CGAGGAGAAA GCCACTGGGG AAAATCAGAG TAGAGATCGG 
AGAAAAATTG ATCAGCACAC ATTACTCCAA AGCATCGTAA ACAGAACTGA CTTGGATCCA 
CGTGTCCTCT CCAACTCTGG TTGGGGACAG ACTCCTATTA AGCAGAATAC TGCCTGGGAT 
ACGGAAACAT CACCTAGAGG GGAAAGAAAG ACTGACAATG GGACAGAGGC CTGGGGAAGC 
TCTGCAACAC AGACTTTTAA CTCAGGGGCA TGTACAGATA AGACTAGCCC TACTAGTAAT 
GATACCTCAT CTGTATCGGG GTGGGGAGAT CCCAAACCTG CTCTGAGGTG GGGAGATTCC 
AAAGGCTCAA GCTGCCAGGG GGGATGGGAA GATGATGCTG CTGCTACAGG AATGGTCAAG 
AGCAATCAGT GGGGGAACAG CAAAGAAGAG AAGTCCGCAT GGAATGATTC GCAAAAGAGC 
AAACAGGGAT GGGGTGATGG ACAGAAATCA AACCAAGGGT GGTCCGTTTC TGCCAGTGAT 
AACTGGGGAG AAACGTCAAG GAGTAACCAT TGGGGTGAGG CTAATAAGAA ATCTAGCTCA 
GGAGGTAGTG ACAGTGACAG GTCTGTTTCT GGTTGGAATG AACTTGGTAA AACTAGTTCT 
TTTACTTGGG GAAATAACAT AAATCCAAAT AATTCGTCAG GATGGGATGA ATCTTCTAAA 
CCTAATCCTT CCCAGGGATG GGGAGATCCT CCAAAGTCTA ATCAGTCTCT AGGTTGGGGA 
GATTCGTCAA AGCCAGTCAC TTCTCCAGAC TGGAACAAGC AACAAGACAT TGTCGGGTCT 
TGGGGAATCC CACCAGCAAC AGGCAAACCT CCTGGTACAG GCTGGCTGGG AGGACCTATA 
CCAGCCCCAA CAAAAGAAGA AGAGCCCACA GGCTGGGAGG AACCATCCCC AGAATCCATA 
CGTCGCAAAA TGGAGATCGA TGACGGAACT TCAGCTTGGG GAGATCCGAG CAAATACAAC 
TACAAAAATG TGAACATGTG GAACAAAAAT GTCCCAAATG GCAGCAGCCG TTCAGACCAG 
CAAGCACAGG TACACCAGTT GCTACCATCT GCAAGTGCCA TCTCAAACAA AGAGGCAGGC 
AGTGGCTCTG GCTGGGGTGA GCCCTGGGGG GAGCCTTCTA CTCCAGCCAC AACTGTGGAT 
AATGGTACTT CAGCATGGGG TAAACCCATA GACAGTGGTC CCAGCTGGGG GGAGCCCATT 
GCTGCAGCAT CCAACACATC CACGTGGGGC TCCAGCTCTG TTGGTCCTCA AGCATTAAGC 
AAATCTGGGC CAAAATCTAT GCAAGATGGC TGGTGTGGTG ATGATATGCC ATTGCCTGGA 
AATCGCCCCA CTGGCTGGGA AGAGGAAGAG GATGTAGAGA TTGGAATGTG GAATAGTAAT 
TCATCTCAAG AGCTTAACTC ATCTTTAAAT TGGCCTCCAT ATACAAAGAA AATGTCATCG 
AAGGGTCTGA GTGGCAAAAA AAGGAGAAGG GAAAGGGGAA CCATGAAAGG TGGAAACAAA 
CAAGAAGAAG CATGGATAAA TCCATTCGTT AAACAGTTTT CAAATATCAG TTTTTCGAGA 
GACTCACCCG AAGAAAATGT ACAGAGCAAT AAGATGGATC TTTCCGGAGG AATGTTACAG 
GACAAACGAA TGGAGATAGA TAAACATAGC CTAAATATTG GTGATTACAA TCGAACGGTC 
GGGAAAGGCC CTGGTTCTCG GCCTCAGATT TCCAAAGAGT CTTCCATGGA GCGCAGCCCT 
TACTTTGATA AGGATGGCAT TGTAGCAGAT GAATCCCAAA ACATGCAGTT TATGTCCAGT 
CAAAGCATGA AGCTTCCCCC TTCAAATAGT GCACTACCTA ACCAGGCCCT TGGCTCCATA 
GCAGGGCTGG GTATGCAAAA CTTGAATTCT GTTAGACAGA ATGGCAATCC CAGTATGTTT 
GGTGTTGGAA ACACAGCAGC ACAACCCCGG GGCATGCAGC AGCCTCCAGC ACAACCTCTT 
AGTTCATCTC AGCCTAATCT TCGTGCTCAA GTGCCTCCTC CATTACTCTC CCCTCAGGTT 
CCAGTTTCAT TGCTGAAGTA TGCACCAAAC AACGGTGGCC TGAATCCGCT CTTTGGCCCT 
CAACAGGTAG CCATGCTGAA CCAGCTATCC CAACTAAACC AGCTTTCTCA GATCTCCCAG 
TTACAGAGAT TGTTAGCGCA GCAGCAAAGG GCGCAGAGTC AGAGAAGCGT GCCTTCTGGG 
AACCGGCAGC AGCAAGACCA GCAGGGTCGA CCTCTTAGCG TGCAGCAGCA GATGATGCAG 
CAGTCCCGGC AACTCGATCC CAGCCTGTTG GTGAAGCAGC AGACCCCGCC ATCTCAGCAG 
CAGCCACTCC ATCAGCCAGC CATGAAATCC TTCCTCGAGA ATGTCATGCC CCACACTACA 
CCTGAGCTGC AGAAAGGGCC ATCACCAATA AATGCTTTCA GCAACTTCCC GATAGGCTTG 
AACTCAAACT TGAATGTAAA TATGGATATG AACAGTATTA AAGAGCCACA GTCAAGACTA 
AGGAAGTGGA CTACAGTGGA CAGCATTTCT GTGAACACAT CTTTGGATCA AAACTCCAGC 
AAACATGGTG CTATTTCAAG TGGTTTCAGG CTGGAAGAGT CTCCATTTGT TCCCTATGAC 
TTTATGAACA GCAGTACTTC TCCAGCCAGC CCTCCGGGTT CAATAGGAGA TGGCTGGCCA 
CGTGCCAAAT CGCCTAACGG CTCTAGCAGT GTTAACTGGC CACCAGAATT TCGCCCTGGT 
GAACCATGGA AAGGTTATCC AAACATTGAC CCTGAAACTG ACCCTTACGT CACTCCTGGC 
AGTGTCATAA ACAATCTTTC AATTAATACT GTGCGGGAAG TTGACCACCT CAGGGACAGG 
AACAGTGGGT CATCCTCATC CTTGAACACC ACGCTGCCTT CAACTAGTGC CTGGTCATCC 
ATTCGTGCCT CCAACTACAA CGTTCCCCTC AGCAGTACAG CACAAAGCAC TTCAGCCAGA 
AATAGTGATT CCAAATTGAC ATGGTCTCCT GGTTCAGTTA CAAACACCTC TCTGGCTCAT 
GAGCTGTGGA AGGTCCCTTT ACCACCTAAA AACATCACTG CTCCGTCCCG CCCACCTCCG 
GGACTCACTG GTCAGAAGCC ACCCTTGTCT ACGTGGGATA ACTCTCCCCT TCGTGTAGGT 
GGAGGATGGG GAAATTCTGA CGCCAGGTAC ACCCCAGGTT CCAGTTGGGG TGAGAGCAGC 
TCAGGGAGAA TAACAAATTG GCTTGTTCTA AAGAACCTCA CACCTCAGAT TGACGGCTCA 
ACTCTGCGTA CTCTGTGCAT GCAGCATGGC CCACTGATAA CATTCCACCT GAACCTCCCA 
CATGGAAATG CTTTGGTCCG TTACAGTTCA AAAGAAGAGG TAGTGAAGGC ACAAAAGTCT 
CTGCACATGT GTGTATTGGG GAACACTACT ATTCTTGCTG AGTTTGCCAG TGAAGAGGAG 
ATCAGCCGTT TCTTTGCACA AAGTCAGTCT CTGACCCCTT CTCCCGGCTG GCAGTCTCTC 
GGGTCCAGCC AGAGCCGGCT GGGCTCCCTC GACTGTTCCC ACTCGTTCTC CAGCCGGACC 
GATCTCAATC ACTGGAATGG TGCTGGGCTG TCGGGAACTA ACTGTGGAGA CCTTCACGGC 
ACTTCACTCT GGGGGACCCC ACATTATTCC ACAAGCCTGT GGGGTCCTCC CAGCAGCAGC 
GACCCCCGGG GAATTAGCAG CCCATCCCCC ATTAATGCTT TTCTTTCTGT TGACCACCTG 
GGTGGGGGTG GAGAGTCCAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970010.1
CDS	276..6179
Translation	MQLVAEPGLEARCPGSQDIPLPEEWNRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNPQQQQQPQPPQQPQQPPQQPPQPPQQQPQPAQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLSSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSPSDSSTNCKNAVVNDSPETEAWPSVPGSDPELASECMDADSASSSESERNITIMASGSTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSPGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLDNNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVGGSSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVMNGTSLSHLSNGEPKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGINGPMGTNFQVNTNKGGGVWESGATNSQNASWGSGNGANSGGSRRGWGTPAQNTGTNIPSVEWSKLPSNQHSNDSANGNGKKFTNGWKSTEEEDQASAASQTTEQNSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACTDKTSPTSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDAAATGMVKSNQWGNSKEEKSAWNDSQKSKQGWGDGQKSNQGWSVSASDNWGETSRSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVTSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPTKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSASAISNKEAGSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASNTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGTMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERSPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPSLLVKQQTPPSQQQPLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_014114535.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014114535.2

ORF Insert Sequence:

ATGCAACTAG TGGCAGAGCC AGGCCTAGAA GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCC AGTGTAAGCC AGCCTCAGCC TGCCAACTCT 
AATAACGGCA CTTCCACAGC AACCAGCACT AATAATAATG CCAAGCGAGC CACAGCCAAC 
AATCCGCAGC AGCAGCAGCA GCCACAGCCG CCACAGCAGC CACAGCAGCC GCCGCAGCAG 
CCACCGCAGC CGCCACAGCA GCAGCCGCAG CCAGCACAGG CCTTGCCTCG GTATCCTCGG 
GAAGTACCAC CACGATTTCG CCACCAGGAA CACAAACAGC TTCTAAAGAG GGGTCAGCAT 
TTTCCTGTCA TAGCAGCAAA CCTGGGATCT GCTGTTAAAG TGTTAAGCAG CCAGTCAGAA 
AGCAGTGCTT TAACAAATCA ACAGCCACAA AATAACGGAG AGGTGCAGAA CAGCAAAAAC 
CAGTCAGATA TAAACCACAG TACTTCAGGA TCCCATTATG AAAATTCACA GCGGGGACCT 
GTGTCTTCTC CGAGTGACTC TAGCACAAAT TGTAAGAATG CTGTTGTAAA CGACTCCCCT 
GAGACAGAAG CATGGCCCTC AGTCCCTGGC AGTGATCCAG AGTTGGCTTC AGAATGTATG 
GATGCTGATT CTGCCTCCAG TTCTGAATCA GAGAGAAACA TCACTATCAT GGCTTCAGGG 
AGCACAGGTG GTGAAAAAGA TGGCCTTCGG AATAGCACTG GACTTGGTTC CCAAAACAAG 
TTTGTGGTTG GTAGCAGCAG CAATAATGTG GGCCATGGAA GTAGTCCTGG GCCATGGGGT 
TTTTCCCATG GAGCCATAAT AAGCACATGT CAGGTCTCTG TGGATGCTCC TGAAAGCAAA 
TCAGAAAGTA GCAACAATAG AATGAATGCT TGGGGCACTG TAAGTTCTTC ATCAAATGGA 
GGGTTAAATC CAAGCACTTT GAATTCAGCT AGCAACCATG GTGCCTGGCC AGTATTAGAC 
AACAACGGAC TTGCCCTCAA AGGGCCTGTA GGGAGCGGTA GTTCTGGCAT CAATATTCAG 
TGTAGTACCA TAGGCCAGAT GCCTAACAAT CAGAGTATTA ACTCTAAAGT GGGTGGTTCT 
TCCACCCATG GTACCTGGGG AAGCCTTCAG GAAACTTGTG AATCTGAAGT AAGTGGTACA 
CAGAAGGTTT CATTCAGTGG TCAACCTCAG AATATTACCA CTGAGATGAC TGGACCAAAT 
AACACTACTA ACTTTATGAC CTCTAGTTTA CCAAACTCCG GTTCAGTACA GAATAACGAA 
CTGCCTAGTA ATACAGGGGC CTGGCGTGTG AGCACAATGA ATCATCCTCA GATACAGGCT 
CCATCGGTTA TGAATGGCAC TTCCCTTTCT CATCTTAGCA ATGGAGAGCC AAAAAGTGGA 
GGCTCTTACG GTACTACGTG GGGTGCCTAT GGTTCTAATT ACTCTGGAGA CAAATGTTCG 
AGCCCTAACG GCCAAGCTAA TGGTGACACT GTGAATGCAA CTCTAATGCA GCCTGGCATA 
AATGGGCCTA TGGGCACTAA CTTTCAAGTG AACACAAATA AAGGAGGAGG TGTGTGGGAG 
TCTGGGGCAA CGAATTCCCA GAATGCATCG TGGGGAAGTG GAAATGGCGC AAATTCTGGA 
GGAAGTCGAC GAGGATGGGG AACTCCTGCA CAAAACACTG GCACTAATAT ACCCAGCGTG 
GAGTGGAGCA AACTGCCTAG CAATCAGCAT TCCAATGACA GTGCAAATGG CAATGGTAAG 
AAGTTTACAA ATGGATGGAA ATCTACTGAG GAAGAGGATC AGGCTTCTGC CGCATCTCAG 
ACAACTGAGC AGAACAGTGT GTGGGCCAAA ACAGGAGGTA CAGTGGAGAG TGAAGGTAGT 
ACAGAAAGCA CTGGACGCCT CGAGGAGAAA GCCACTGGGG AAAATCAGAG TAGAGATCGG 
AGAAAAATTG ATCAGCACAC ATTACTCCAA AGCATCGTAA ACAGAACTGA CTTGGATCCA 
CGTGTCCTCT CCAACTCTGG TTGGGGACAG ACTCCTATTA AGCAGAATAC TGCCTGGGAT 
ACGGAAACAT CACCTAGAGG GGAAAGAAAG ACTGACAATG GGACAGAGGC CTGGGGAAGC 
TCTGCAACAC AGACTTTTAA CTCAGGGGCA TGTACAGATA AGACTAGCCC TACTAGTAAT 
GATACCTCAT CTGTATCGGG GTGGGGAGAT CCCAAACCTG CTCTGAGGTG GGGAGATTCC 
AAAGGCTCAA GCTGCCAGGG GGGATGGGAA GATGATGCTG CTGCTACAGG AATGGTCAAG 
AGCAATCAGT GGGGGAACAG CAAAGAAGAG AAGTCCGCAT GGAATGATTC GCAAAAGAGC 
AAACAGGGAT GGGGTGATGG ACAGAAATCA AACCAAGGGT GGTCCGTTTC TGCCAGTGAT 
AACTGGGGAG AAACGTCAAG GAGTAACCAT TGGGGTGAGG CTAATAAGAA ATCTAGCTCA 
GGAGGTAGTG ACAGTGACAG GTCTGTTTCT GGTTGGAATG AACTTGGTAA AACTAGTTCT 
TTTACTTGGG GAAATAACAT AAATCCAAAT AATTCGTCAG GATGGGATGA ATCTTCTAAA 
CCTAATCCTT CCCAGGGATG GGGAGATCCT CCAAAGTCTA ATCAGTCTCT AGGTTGGGGA 
GATTCGTCAA AGCCAGTCAC TTCTCCAGAC TGGAACAAGC AACAAGACAT TGTCGGGTCT 
TGGGGAATCC CACCAGCAAC AGGCAAACCT CCTGGTACAG GCTGGCTGGG AGGACCTATA 
CCAGCCCCAA CAAAAGAAGA AGAGCCCACA GGCTGGGAGG AACCATCCCC AGAATCCATA 
CGTCGCAAAA TGGAGATCGA TGACGGAACT TCAGCTTGGG GAGATCCGAG CAAATACAAC 
TACAAAAATG TGAACATGTG GAACAAAAAT GTCCCAAATG GCAGCAGCCG TTCAGACCAG 
CAAGCACAGG TACACCAGTT GCTACCATCT GCAAGTGCCA TCTCAAACAA AGAGGCAGGC 
AGTGGCTCTG GCTGGGGTGA GCCCTGGGGG GAGCCTTCTA CTCCAGCCAC AACTGTGGAT 
AATGGTACTT CAGCATGGGG TAAACCCATA GACAGTGGTC CCAGCTGGGG GGAGCCCATT 
GCTGCAGCAT CCAACACATC CACGTGGGGC TCCAGCTCTG TTGGTCCTCA AGCATTAAGC 
AAATCTGGGC CAAAATCTAT GCAAGATGGC TGGTGTGGTG ATGATATGCC ATTGCCTGGA 
AATCGCCCCA CTGGCTGGGA AGAGGAAGAG GATGTAGAGA TTGGAATGTG GAATAGTAAT 
TCATCTCAAG AGCTTAACTC ATCTTTAAAT TGGCCTCCAT ATACAAAGAA AATGTCATCG 
AAGGGTCTGA GTGGCAAAAA AAGGAGAAGG GAAAGGGGAA CCATGAAAGG TGGAAACAAA 
CAAGAAGAAG CATGGATAAA TCCATTCGTT AAACAGTTTT CAAATATCAG TTTTTCGAGA 
GACTCACCCG AAGAAAATGT ACAGAGCAAT AAGATGGATC TTTCCGGAGG AATGTTACAG 
GACAAACGAA TGGAGATAGA TAAACATAGC CTAAATATTG GTGATTACAA TCGAACGGTC 
GGGAAAGGCC CTGGTTCTCG GCCTCAGATT TCCAAAGAGT CTTCCATGGA GCGCAGCCCT 
TACTTTGATA AGGATGGCAT TGTAGCAGAT GAATCCCAAA ACATGCAGTT TATGTCCAGT 
CAAAGCATGA AGCTTCCCCC TTCAAATAGT GCACTACCTA ACCAGGCCCT TGGCTCCATA 
GCAGGGCTGG GTATGCAAAA CTTGAATTCT GTTAGACAGA ATGGCAATCC CAGTATGTTT 
GGTGTTGGAA ACACAGCAGC ACAACCCCGG GGCATGCAGC AGCCTCCAGC ACAACCTCTT 
AGTTCATCTC AGCCTAATCT TCGTGCTCAA GTGCCTCCTC CATTACTCTC CCCTCAGGTT 
CCAGTTTCAT TGCTGAAGTA TGCACCAAAC AACGGTGGCC TGAATCCGCT CTTTGGCCCT 
CAACAGGTAG CCATGCTGAA CCAGCTATCC CAACTAAACC AGCTTTCTCA GATCTCCCAG 
TTACAGAGAT TGTTAGCGCA GCAGCAAAGG GCGCAGAGTC AGAGAAGCGT GCCTTCTGGG 
AACCGGCAGC AGCAAGACCA GCAGGGTCGA CCTCTTAGCG TGCAGCAGCA GATGATGCAG 
CAGTCCCGGC AACTCGATCC CAGCCTGTTG GTGAAGCAGC AGACCCCGCC ATCTCAGCAG 
CAGCCACTCC ATCAGCCAGC CATGAAATCC TTCCTCGAGA ATGTCATGCC CCACACTACA 
CCTGAGCTGC AGAAAGGGCC ATCACCAATA AATGCTTTCA GCAACTTCCC GATAGGCTTG 
AACTCAAACT TGAATGTAAA TATGGATATG AACAGTATTA AAGAGCCACA GTCAAGACTA 
AGGAAGTGGA CTACAGTGGA CAGCATTTCT GTGAACACAT CTTTGGATCA AAACTCCAGC 
AAACATGGTG CTATTTCAAG TGGTTTCAGG CTGGAAGAGT CTCCATTTGT TCCCTATGAC 
TTTATGAACA GCAGTACTTC TCCAGCCAGC CCTCCGGGTT CAATAGGAGA TGGCTGGCCA 
CGTGCCAAAT CGCCTAACGG CTCTAGCAGT GTTAACTGGC CACCAGAATT TCGCCCTGGT 
GAACCATGGA AAGGTTATCC AAACATTGAC CCTGAAACTG ACCCTTACGT CACTCCTGGC 
AGTGTCATAA ACAATCTTTC AATTAATACT GTGCGGGAAG TTGACCACCT CAGGGACAGG 
AACAGTGGGT CATCCTCATC CTTGAACACC ACGCTGCCTT CAACTAGTGC CTGGTCATCC 
ATTCGTGCCT CCAACTACAA CGTTCCCCTC AGCAGTACAG CACAAAGCAC TTCAGCCAGA 
AATAGTGATT CCAAATTGAC ATGGTCTCCT GGTTCAGTTA CAAACACCTC TCTGGCTCAT 
GAGCTGTGGA AGGTCCCTTT ACCACCTAAA AACATCACTG CTCCGTCCCG CCCACCTCCG 
GGACTCACTG GTCAGAAGCC ACCCTTGTCT ACGTGGGATA ACTCTCCCCT TCGTGTAGGT 
GGAGGATGGG GAAATTCTGA CGCCAGGTAC ACCCCAGGTT CCAGTTGGGG TGAGAGCAGC 
TCAGGGAGAA TAACAAATTG GCTTGTTCTA AAGAACCTCA CACCTCAGAT TGACGGCTCA 
ACTCTGCGTA CTCTGTGCAT GCAGCATGGC CCACTGATAA CATTCCACCT GAACCTCCCA 
CATGGAAATG CTTTGGTCCG TTACAGTTCA AAAGAAGAGG TAGTGAAGGC ACAAAAGTCT 
CTGCACATGT GTGTATTGGG GAACACTACT ATTCTTGCTG AGTTTGCCAG TGAAGAGGAG 
ATCAGCCGTT TCTTTGCACA AAGTCAGTCT CTGACCCCTT CTCCCGGCTG GCAGTCTCTC 
GGGTCCAGCC AGAGCCGGCT GGGCTCCCTC GACTGTTCCC ACTCGTTCTC CAGCCGGACC 
GATCTCAATC ACTGGAATGG TGCTGGGCTG TCGGGAACTA ACTGTGGAGA CCTTCACGGC 
ACTTCACTCT GGGGGACCCC ACATTATTCC ACAAGCCTGT GGGGTCCTCC CAGCAGCAGC 
GACCCCCGGG GAATTAGCAG CCCATCCCCC ATTAATGCTT TTCTTTCTGT TGACCACCTG 
GGTGGGGGTG GAGAGTCCAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_014114535.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013970010.1 Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X2, mRNA.

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CloneID	OCe37067
Clone ID Related Accession (Same CDS sequence)	XM_014114535.2 , XM_014114535.1
Accession Version	XM_014114535.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5904bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	trinucleotide repeat-containing gene 6A protein isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACTAG TGGCAGAGCC AGGCCTAGAA GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCC AGTGTAAGCC AGCCTCAGCC TGCCAACTCT 
AATAACGGCA CTTCCACAGC AACCAGCACT AATAATAATG CCAAGCGAGC CACAGCCAAC 
AATCCGCAGC AGCAGCAGCA GCCACAGCCG CCACAGCAGC CACAGCAGCC GCCGCAGCAG 
CCACCGCAGC CGCCACAGCA GCAGCCGCAG CCAGCACAGG CCTTGCCTCG GTATCCTCGG 
GAAGTACCAC CACGATTTCG CCACCAGGAA CACAAACAGC TTCTAAAGAG GGGTCAGCAT 
TTTCCTGTCA TAGCAGCAAA CCTGGGATCT GCTGTTAAAG TGTTAAGCAG CCAGTCAGAA 
AGCAGTGCTT TAACAAATCA ACAGCCACAA AATAACGGAG AGGTGCAGAA CAGCAAAAAC 
CAGTCAGATA TAAACCACAG TACTTCAGGA TCCCATTATG AAAATTCACA GCGGGGACCT 
GTGTCTTCTC CGAGTGACTC TAGCACAAAT TGTAAGAATG CTGTTGTAAA CGACTCCCCT 
GAGACAGAAG CATGGCCCTC AGTCCCTGGC AGTGATCCAG AGTTGGCTTC AGAATGTATG 
GATGCTGATT CTGCCTCCAG TTCTGAATCA GAGAGAAACA TCACTATCAT GGCTTCAGGG 
AGCACAGGTG GTGAAAAAGA TGGCCTTCGG AATAGCACTG GACTTGGTTC CCAAAACAAG 
TTTGTGGTTG GTAGCAGCAG CAATAATGTG GGCCATGGAA GTAGTCCTGG GCCATGGGGT 
TTTTCCCATG GAGCCATAAT AAGCACATGT CAGGTCTCTG TGGATGCTCC TGAAAGCAAA 
TCAGAAAGTA GCAACAATAG AATGAATGCT TGGGGCACTG TAAGTTCTTC ATCAAATGGA 
GGGTTAAATC CAAGCACTTT GAATTCAGCT AGCAACCATG GTGCCTGGCC AGTATTAGAC 
AACAACGGAC TTGCCCTCAA AGGGCCTGTA GGGAGCGGTA GTTCTGGCAT CAATATTCAG 
TGTAGTACCA TAGGCCAGAT GCCTAACAAT CAGAGTATTA ACTCTAAAGT GGGTGGTTCT 
TCCACCCATG GTACCTGGGG AAGCCTTCAG GAAACTTGTG AATCTGAAGT AAGTGGTACA 
CAGAAGGTTT CATTCAGTGG TCAACCTCAG AATATTACCA CTGAGATGAC TGGACCAAAT 
AACACTACTA ACTTTATGAC CTCTAGTTTA CCAAACTCCG GTTCAGTACA GAATAACGAA 
CTGCCTAGTA ATACAGGGGC CTGGCGTGTG AGCACAATGA ATCATCCTCA GATACAGGCT 
CCATCGGTTA TGAATGGCAC TTCCCTTTCT CATCTTAGCA ATGGAGAGCC AAAAAGTGGA 
GGCTCTTACG GTACTACGTG GGGTGCCTAT GGTTCTAATT ACTCTGGAGA CAAATGTTCG 
AGCCCTAACG GCCAAGCTAA TGGTGACACT GTGAATGCAA CTCTAATGCA GCCTGGCATA 
AATGGGCCTA TGGGCACTAA CTTTCAAGTG AACACAAATA AAGGAGGAGG TGTGTGGGAG 
TCTGGGGCAA CGAATTCCCA GAATGCATCG TGGGGAAGTG GAAATGGCGC AAATTCTGGA 
GGAAGTCGAC GAGGATGGGG AACTCCTGCA CAAAACACTG GCACTAATAT ACCCAGCGTG 
GAGTGGAGCA AACTGCCTAG CAATCAGCAT TCCAATGACA GTGCAAATGG CAATGGTAAG 
AAGTTTACAA ATGGATGGAA ATCTACTGAG GAAGAGGATC AGGCTTCTGC CGCATCTCAG 
ACAACTGAGC AGAACAGTGT GTGGGCCAAA ACAGGAGGTA CAGTGGAGAG TGAAGGTAGT 
ACAGAAAGCA CTGGACGCCT CGAGGAGAAA GCCACTGGGG AAAATCAGAG TAGAGATCGG 
AGAAAAATTG ATCAGCACAC ATTACTCCAA AGCATCGTAA ACAGAACTGA CTTGGATCCA 
CGTGTCCTCT CCAACTCTGG TTGGGGACAG ACTCCTATTA AGCAGAATAC TGCCTGGGAT 
ACGGAAACAT CACCTAGAGG GGAAAGAAAG ACTGACAATG GGACAGAGGC CTGGGGAAGC 
TCTGCAACAC AGACTTTTAA CTCAGGGGCA TGTACAGATA AGACTAGCCC TACTAGTAAT 
GATACCTCAT CTGTATCGGG GTGGGGAGAT CCCAAACCTG CTCTGAGGTG GGGAGATTCC 
AAAGGCTCAA GCTGCCAGGG GGGATGGGAA GATGATGCTG CTGCTACAGG AATGGTCAAG 
AGCAATCAGT GGGGGAACAG CAAAGAAGAG AAGTCCGCAT GGAATGATTC GCAAAAGAGC 
AAACAGGGAT GGGGTGATGG ACAGAAATCA AACCAAGGGT GGTCCGTTTC TGCCAGTGAT 
AACTGGGGAG AAACGTCAAG GAGTAACCAT TGGGGTGAGG CTAATAAGAA ATCTAGCTCA 
GGAGGTAGTG ACAGTGACAG GTCTGTTTCT GGTTGGAATG AACTTGGTAA AACTAGTTCT 
TTTACTTGGG GAAATAACAT AAATCCAAAT AATTCGTCAG GATGGGATGA ATCTTCTAAA 
CCTAATCCTT CCCAGGGATG GGGAGATCCT CCAAAGTCTA ATCAGTCTCT AGGTTGGGGA 
GATTCGTCAA AGCCAGTCAC TTCTCCAGAC TGGAACAAGC AACAAGACAT TGTCGGGTCT 
TGGGGAATCC CACCAGCAAC AGGCAAACCT CCTGGTACAG GCTGGCTGGG AGGACCTATA 
CCAGCCCCAA CAAAAGAAGA AGAGCCCACA GGCTGGGAGG AACCATCCCC AGAATCCATA 
CGTCGCAAAA TGGAGATCGA TGACGGAACT TCAGCTTGGG GAGATCCGAG CAAATACAAC 
TACAAAAATG TGAACATGTG GAACAAAAAT GTCCCAAATG GCAGCAGCCG TTCAGACCAG 
CAAGCACAGG TACACCAGTT GCTACCATCT GCAAGTGCCA TCTCAAACAA AGAGGCAGGC 
AGTGGCTCTG GCTGGGGTGA GCCCTGGGGG GAGCCTTCTA CTCCAGCCAC AACTGTGGAT 
AATGGTACTT CAGCATGGGG TAAACCCATA GACAGTGGTC CCAGCTGGGG GGAGCCCATT 
GCTGCAGCAT CCAACACATC CACGTGGGGC TCCAGCTCTG TTGGTCCTCA AGCATTAAGC 
AAATCTGGGC CAAAATCTAT GCAAGATGGC TGGTGTGGTG ATGATATGCC ATTGCCTGGA 
AATCGCCCCA CTGGCTGGGA AGAGGAAGAG GATGTAGAGA TTGGAATGTG GAATAGTAAT 
TCATCTCAAG AGCTTAACTC ATCTTTAAAT TGGCCTCCAT ATACAAAGAA AATGTCATCG 
AAGGGTCTGA GTGGCAAAAA AAGGAGAAGG GAAAGGGGAA CCATGAAAGG TGGAAACAAA 
CAAGAAGAAG CATGGATAAA TCCATTCGTT AAACAGTTTT CAAATATCAG TTTTTCGAGA 
GACTCACCCG AAGAAAATGT ACAGAGCAAT AAGATGGATC TTTCCGGAGG AATGTTACAG 
GACAAACGAA TGGAGATAGA TAAACATAGC CTAAATATTG GTGATTACAA TCGAACGGTC 
GGGAAAGGCC CTGGTTCTCG GCCTCAGATT TCCAAAGAGT CTTCCATGGA GCGCAGCCCT 
TACTTTGATA AGGATGGCAT TGTAGCAGAT GAATCCCAAA ACATGCAGTT TATGTCCAGT 
CAAAGCATGA AGCTTCCCCC TTCAAATAGT GCACTACCTA ACCAGGCCCT TGGCTCCATA 
GCAGGGCTGG GTATGCAAAA CTTGAATTCT GTTAGACAGA ATGGCAATCC CAGTATGTTT 
GGTGTTGGAA ACACAGCAGC ACAACCCCGG GGCATGCAGC AGCCTCCAGC ACAACCTCTT 
AGTTCATCTC AGCCTAATCT TCGTGCTCAA GTGCCTCCTC CATTACTCTC CCCTCAGGTT 
CCAGTTTCAT TGCTGAAGTA TGCACCAAAC AACGGTGGCC TGAATCCGCT CTTTGGCCCT 
CAACAGGTAG CCATGCTGAA CCAGCTATCC CAACTAAACC AGCTTTCTCA GATCTCCCAG 
TTACAGAGAT TGTTAGCGCA GCAGCAAAGG GCGCAGAGTC AGAGAAGCGT GCCTTCTGGG 
AACCGGCAGC AGCAAGACCA GCAGGGTCGA CCTCTTAGCG TGCAGCAGCA GATGATGCAG 
CAGTCCCGGC AACTCGATCC CAGCCTGTTG GTGAAGCAGC AGACCCCGCC ATCTCAGCAG 
CAGCCACTCC ATCAGCCAGC CATGAAATCC TTCCTCGAGA ATGTCATGCC CCACACTACA 
CCTGAGCTGC AGAAAGGGCC ATCACCAATA AATGCTTTCA GCAACTTCCC GATAGGCTTG 
AACTCAAACT TGAATGTAAA TATGGATATG AACAGTATTA AAGAGCCACA GTCAAGACTA 
AGGAAGTGGA CTACAGTGGA CAGCATTTCT GTGAACACAT CTTTGGATCA AAACTCCAGC 
AAACATGGTG CTATTTCAAG TGGTTTCAGG CTGGAAGAGT CTCCATTTGT TCCCTATGAC 
TTTATGAACA GCAGTACTTC TCCAGCCAGC CCTCCGGGTT CAATAGGAGA TGGCTGGCCA 
CGTGCCAAAT CGCCTAACGG CTCTAGCAGT GTTAACTGGC CACCAGAATT TCGCCCTGGT 
GAACCATGGA AAGGTTATCC AAACATTGAC CCTGAAACTG ACCCTTACGT CACTCCTGGC 
AGTGTCATAA ACAATCTTTC AATTAATACT GTGCGGGAAG TTGACCACCT CAGGGACAGG 
AACAGTGGGT CATCCTCATC CTTGAACACC ACGCTGCCTT CAACTAGTGC CTGGTCATCC 
ATTCGTGCCT CCAACTACAA CGTTCCCCTC AGCAGTACAG CACAAAGCAC TTCAGCCAGA 
AATAGTGATT CCAAATTGAC ATGGTCTCCT GGTTCAGTTA CAAACACCTC TCTGGCTCAT 
GAGCTGTGGA AGGTCCCTTT ACCACCTAAA AACATCACTG CTCCGTCCCG CCCACCTCCG 
GGACTCACTG GTCAGAAGCC ACCCTTGTCT ACGTGGGATA ACTCTCCCCT TCGTGTAGGT 
GGAGGATGGG GAAATTCTGA CGCCAGGTAC ACCCCAGGTT CCAGTTGGGG TGAGAGCAGC 
TCAGGGAGAA TAACAAATTG GCTTGTTCTA AAGAACCTCA CACCTCAGAT TGACGGCTCA 
ACTCTGCGTA CTCTGTGCAT GCAGCATGGC CCACTGATAA CATTCCACCT GAACCTCCCA 
CATGGAAATG CTTTGGTCCG TTACAGTTCA AAAGAAGAGG TAGTGAAGGC ACAAAAGTCT 
CTGCACATGT GTGTATTGGG GAACACTACT ATTCTTGCTG AGTTTGCCAG TGAAGAGGAG 
ATCAGCCGTT TCTTTGCACA AAGTCAGTCT CTGACCCCTT CTCCCGGCTG GCAGTCTCTC 
GGGTCCAGCC AGAGCCGGCT GGGCTCCCTC GACTGTTCCC ACTCGTTCTC CAGCCGGACC 
GATCTCAATC ACTGGAATGG TGCTGGGCTG TCGGGAACTA ACTGTGGAGA CCTTCACGGC 
ACTTCACTCT GGGGGACCCC ACATTATTCC ACAAGCCTGT GGGGTCCTCC CAGCAGCAGC 
GACCCCCGGG GAATTAGCAG CCCATCCCCC ATTAATGCTT TTCTTTCTGT TGACCACCTG 
GGTGGGGGTG GAGAGTCCAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970010.1
CDS	283..6186
Translation	MQLVAEPGLEARCPGSQDIPLPEEWNRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNPQQQQQPQPPQQPQQPPQQPPQPPQQQPQPAQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLSSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSPSDSSTNCKNAVVNDSPETEAWPSVPGSDPELASECMDADSASSSESERNITIMASGSTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSPGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLDNNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVGGSSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVMNGTSLSHLSNGEPKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGINGPMGTNFQVNTNKGGGVWESGATNSQNASWGSGNGANSGGSRRGWGTPAQNTGTNIPSVEWSKLPSNQHSNDSANGNGKKFTNGWKSTEEEDQASAASQTTEQNSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACTDKTSPTSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDAAATGMVKSNQWGNSKEEKSAWNDSQKSKQGWGDGQKSNQGWSVSASDNWGETSRSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVTSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPTKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSASAISNKEAGSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASNTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGTMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERSPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPSLLVKQQTPPSQQQPLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_014114535.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014114535.1

ORF Insert Sequence:

ATGCAACTAG TGGCAGAGCC AGGCCTAGAA GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCC AGTGTAAGCC AGCCTCAGCC TGCCAACTCT 
AATAACGGCA CTTCCACAGC AACCAGCACT AATAATAATG CCAAGCGAGC CACAGCCAAC 
AATCCGCAGC AGCAGCAGCA GCCACAGCCG CCACAGCAGC CACAGCAGCC GCCGCAGCAG 
CCACCGCAGC CGCCACAGCA GCAGCCGCAG CCAGCACAGG CCTTGCCTCG GTATCCTCGG 
GAAGTACCAC CACGATTTCG CCACCAGGAA CACAAACAGC TTCTAAAGAG GGGTCAGCAT 
TTTCCTGTCA TAGCAGCAAA CCTGGGATCT GCTGTTAAAG TGTTAAGCAG CCAGTCAGAA 
AGCAGTGCTT TAACAAATCA ACAGCCACAA AATAACGGAG AGGTGCAGAA CAGCAAAAAC 
CAGTCAGATA TAAACCACAG TACTTCAGGA TCCCATTATG AAAATTCACA GCGGGGACCT 
GTGTCTTCTC CGAGTGACTC TAGCACAAAT TGTAAGAATG CTGTTGTAAA CGACTCCCCT 
GAGACAGAAG CATGGCCCTC AGTCCCTGGC AGTGATCCAG AGTTGGCTTC AGAATGTATG 
GATGCTGATT CTGCCTCCAG TTCTGAATCA GAGAGAAACA TCACTATCAT GGCTTCAGGG 
AGCACAGGTG GTGAAAAAGA TGGCCTTCGG AATAGCACTG GACTTGGTTC CCAAAACAAG 
TTTGTGGTTG GTAGCAGCAG CAATAATGTG GGCCATGGAA GTAGTCCTGG GCCATGGGGT 
TTTTCCCATG GAGCCATAAT AAGCACATGT CAGGTCTCTG TGGATGCTCC TGAAAGCAAA 
TCAGAAAGTA GCAACAATAG AATGAATGCT TGGGGCACTG TAAGTTCTTC ATCAAATGGA 
GGGTTAAATC CAAGCACTTT GAATTCAGCT AGCAACCATG GTGCCTGGCC AGTATTAGAC 
AACAACGGAC TTGCCCTCAA AGGGCCTGTA GGGAGCGGTA GTTCTGGCAT CAATATTCAG 
TGTAGTACCA TAGGCCAGAT GCCTAACAAT CAGAGTATTA ACTCTAAAGT GGGTGGTTCT 
TCCACCCATG GTACCTGGGG AAGCCTTCAG GAAACTTGTG AATCTGAAGT AAGTGGTACA 
CAGAAGGTTT CATTCAGTGG TCAACCTCAG AATATTACCA CTGAGATGAC TGGACCAAAT 
AACACTACTA ACTTTATGAC CTCTAGTTTA CCAAACTCCG GTTCAGTACA GAATAACGAA 
CTGCCTAGTA ATACAGGGGC CTGGCGTGTG AGCACAATGA ATCATCCTCA GATACAGGCT 
CCATCGGTTA TGAATGGCAC TTCCCTTTCT CATCTTAGCA ATGGAGAGCC AAAAAGTGGA 
GGCTCTTACG GTACTACGTG GGGTGCCTAT GGTTCTAATT ACTCTGGAGA CAAATGTTCG 
AGCCCTAACG GCCAAGCTAA TGGTGACACT GTGAATGCAA CTCTAATGCA GCCTGGCATA 
AATGGGCCTA TGGGCACTAA CTTTCAAGTG AACACAAATA AAGGAGGAGG TGTGTGGGAG 
TCTGGGGCAA CGAATTCCCA GAATGCATCG TGGGGAAGTG GAAATGGCGC AAATTCTGGA 
GGAAGTCGAC GAGGATGGGG AACTCCTGCA CAAAACACTG GCACTAATAT ACCCAGCGTG 
GAGTGGAGCA AACTGCCTAG CAATCAGCAT TCCAATGACA GTGCAAATGG CAATGGTAAG 
AAGTTTACAA ATGGATGGAA ATCTACTGAG GAAGAGGATC AGGCTTCTGC CGCATCTCAG 
ACAACTGAGC AGAACAGTGT GTGGGCCAAA ACAGGAGGTA CAGTGGAGAG TGAAGGTAGT 
ACAGAAAGCA CTGGACGCCT CGAGGAGAAA GCCACTGGGG AAAATCAGAG TAGAGATCGG 
AGAAAAATTG ATCAGCACAC ATTACTCCAA AGCATCGTAA ACAGAACTGA CTTGGATCCA 
CGTGTCCTCT CCAACTCTGG TTGGGGACAG ACTCCTATTA AGCAGAATAC TGCCTGGGAT 
ACGGAAACAT CACCTAGAGG GGAAAGAAAG ACTGACAATG GGACAGAGGC CTGGGGAAGC 
TCTGCAACAC AGACTTTTAA CTCAGGGGCA TGTACAGATA AGACTAGCCC TACTAGTAAT 
GATACCTCAT CTGTATCGGG GTGGGGAGAT CCCAAACCTG CTCTGAGGTG GGGAGATTCC 
AAAGGCTCAA GCTGCCAGGG GGGATGGGAA GATGATGCTG CTGCTACAGG AATGGTCAAG 
AGCAATCAGT GGGGGAACAG CAAAGAAGAG AAGTCCGCAT GGAATGATTC GCAAAAGAGC 
AAACAGGGAT GGGGTGATGG ACAGAAATCA AACCAAGGGT GGTCCGTTTC TGCCAGTGAT 
AACTGGGGAG AAACGTCAAG GAGTAACCAT TGGGGTGAGG CTAATAAGAA ATCTAGCTCA 
GGAGGTAGTG ACAGTGACAG GTCTGTTTCT GGTTGGAATG AACTTGGTAA AACTAGTTCT 
TTTACTTGGG GAAATAACAT AAATCCAAAT AATTCGTCAG GATGGGATGA ATCTTCTAAA 
CCTAATCCTT CCCAGGGATG GGGAGATCCT CCAAAGTCTA ATCAGTCTCT AGGTTGGGGA 
GATTCGTCAA AGCCAGTCAC TTCTCCAGAC TGGAACAAGC AACAAGACAT TGTCGGGTCT 
TGGGGAATCC CACCAGCAAC AGGCAAACCT CCTGGTACAG GCTGGCTGGG AGGACCTATA 
CCAGCCCCAA CAAAAGAAGA AGAGCCCACA GGCTGGGAGG AACCATCCCC AGAATCCATA 
CGTCGCAAAA TGGAGATCGA TGACGGAACT TCAGCTTGGG GAGATCCGAG CAAATACAAC 
TACAAAAATG TGAACATGTG GAACAAAAAT GTCCCAAATG GCAGCAGCCG TTCAGACCAG 
CAAGCACAGG TACACCAGTT GCTACCATCT GCAAGTGCCA TCTCAAACAA AGAGGCAGGC 
AGTGGCTCTG GCTGGGGTGA GCCCTGGGGG GAGCCTTCTA CTCCAGCCAC AACTGTGGAT 
AATGGTACTT CAGCATGGGG TAAACCCATA GACAGTGGTC CCAGCTGGGG GGAGCCCATT 
GCTGCAGCAT CCAACACATC CACGTGGGGC TCCAGCTCTG TTGGTCCTCA AGCATTAAGC 
AAATCTGGGC CAAAATCTAT GCAAGATGGC TGGTGTGGTG ATGATATGCC ATTGCCTGGA 
AATCGCCCCA CTGGCTGGGA AGAGGAAGAG GATGTAGAGA TTGGAATGTG GAATAGTAAT 
TCATCTCAAG AGCTTAACTC ATCTTTAAAT TGGCCTCCAT ATACAAAGAA AATGTCATCG 
AAGGGTCTGA GTGGCAAAAA AAGGAGAAGG GAAAGGGGAA CCATGAAAGG TGGAAACAAA 
CAAGAAGAAG CATGGATAAA TCCATTCGTT AAACAGTTTT CAAATATCAG TTTTTCGAGA 
GACTCACCCG AAGAAAATGT ACAGAGCAAT AAGATGGATC TTTCCGGAGG AATGTTACAG 
GACAAACGAA TGGAGATAGA TAAACATAGC CTAAATATTG GTGATTACAA TCGAACGGTC 
GGGAAAGGCC CTGGTTCTCG GCCTCAGATT TCCAAAGAGT CTTCCATGGA GCGCAGCCCT 
TACTTTGATA AGGATGGCAT TGTAGCAGAT GAATCCCAAA ACATGCAGTT TATGTCCAGT 
CAAAGCATGA AGCTTCCCCC TTCAAATAGT GCACTACCTA ACCAGGCCCT TGGCTCCATA 
GCAGGGCTGG GTATGCAAAA CTTGAATTCT GTTAGACAGA ATGGCAATCC CAGTATGTTT 
GGTGTTGGAA ACACAGCAGC ACAACCCCGG GGCATGCAGC AGCCTCCAGC ACAACCTCTT 
AGTTCATCTC AGCCTAATCT TCGTGCTCAA GTGCCTCCTC CATTACTCTC CCCTCAGGTT 
CCAGTTTCAT TGCTGAAGTA TGCACCAAAC AACGGTGGCC TGAATCCGCT CTTTGGCCCT 
CAACAGGTAG CCATGCTGAA CCAGCTATCC CAACTAAACC AGCTTTCTCA GATCTCCCAG 
TTACAGAGAT TGTTAGCGCA GCAGCAAAGG GCGCAGAGTC AGAGAAGCGT GCCTTCTGGG 
AACCGGCAGC AGCAAGACCA GCAGGGTCGA CCTCTTAGCG TGCAGCAGCA GATGATGCAG 
CAGTCCCGGC AACTCGATCC CAGCCTGTTG GTGAAGCAGC AGACCCCGCC ATCTCAGCAG 
CAGCCACTCC ATCAGCCAGC CATGAAATCC TTCCTCGAGA ATGTCATGCC CCACACTACA 
CCTGAGCTGC AGAAAGGGCC ATCACCAATA AATGCTTTCA GCAACTTCCC GATAGGCTTG 
AACTCAAACT TGAATGTAAA TATGGATATG AACAGTATTA AAGAGCCACA GTCAAGACTA 
AGGAAGTGGA CTACAGTGGA CAGCATTTCT GTGAACACAT CTTTGGATCA AAACTCCAGC 
AAACATGGTG CTATTTCAAG TGGTTTCAGG CTGGAAGAGT CTCCATTTGT TCCCTATGAC 
TTTATGAACA GCAGTACTTC TCCAGCCAGC CCTCCGGGTT CAATAGGAGA TGGCTGGCCA 
CGTGCCAAAT CGCCTAACGG CTCTAGCAGT GTTAACTGGC CACCAGAATT TCGCCCTGGT 
GAACCATGGA AAGGTTATCC AAACATTGAC CCTGAAACTG ACCCTTACGT CACTCCTGGC 
AGTGTCATAA ACAATCTTTC AATTAATACT GTGCGGGAAG TTGACCACCT CAGGGACAGG 
AACAGTGGGT CATCCTCATC CTTGAACACC ACGCTGCCTT CAACTAGTGC CTGGTCATCC 
ATTCGTGCCT CCAACTACAA CGTTCCCCTC AGCAGTACAG CACAAAGCAC TTCAGCCAGA 
AATAGTGATT CCAAATTGAC ATGGTCTCCT GGTTCAGTTA CAAACACCTC TCTGGCTCAT 
GAGCTGTGGA AGGTCCCTTT ACCACCTAAA AACATCACTG CTCCGTCCCG CCCACCTCCG 
GGACTCACTG GTCAGAAGCC ACCCTTGTCT ACGTGGGATA ACTCTCCCCT TCGTGTAGGT 
GGAGGATGGG GAAATTCTGA CGCCAGGTAC ACCCCAGGTT CCAGTTGGGG TGAGAGCAGC 
TCAGGGAGAA TAACAAATTG GCTTGTTCTA AAGAACCTCA CACCTCAGAT TGACGGCTCA 
ACTCTGCGTA CTCTGTGCAT GCAGCATGGC CCACTGATAA CATTCCACCT GAACCTCCCA 
CATGGAAATG CTTTGGTCCG TTACAGTTCA AAAGAAGAGG TAGTGAAGGC ACAAAAGTCT 
CTGCACATGT GTGTATTGGG GAACACTACT ATTCTTGCTG AGTTTGCCAG TGAAGAGGAG 
ATCAGCCGTT TCTTTGCACA AAGTCAGTCT CTGACCCCTT CTCCCGGCTG GCAGTCTCTC 
GGGTCCAGCC AGAGCCGGCT GGGCTCCCTC GACTGTTCCC ACTCGTTCTC CAGCCGGACC 
GATCTCAATC ACTGGAATGG TGCTGGGCTG TCGGGAACTA ACTGTGGAGA CCTTCACGGC 
ACTTCACTCT GGGGGACCCC ACATTATTCC ACAAGCCTGT GGGGTCCTCC CAGCAGCAGC 
GACCCCCGGG GAATTAGCAG CCCATCCCCC ATTAATGCTT TTCTTTCTGT TGACCACCTG 
GGTGGGGGTG GAGAGTCCAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_014114536.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013970011.1 Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X7, mRNA.

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CloneID	OCe37068
Clone ID Related Accession (Same CDS sequence)	XM_014114536.1
Accession Version	XM_014114536.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	trinucleotide repeat-containing gene 6A protein isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACTAG TGGCAGAGCC AGGCCTAGAA GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG AGAATTGGAA GCTAAAGCTA CCAAAGACGT AGAAAGGACT 
CTTAGCAGGG ATTTAGTGCA AGAAGAAGAA CAGTTGATGG AAGAAAAGAA AAAGAAAAAA 
GACGACAAGA AAAAGAAGGA AGCTGCTCAA AAGAAGGCCA CTGAACAAAA AATCAAAGAT 
ATAAACCACA GTACTTCAGG ATCCCATTAT GAAAATTCAC AGCGGGGACC TGTGTCTTCT 
CCGAGTGACT CTAGCACAAA TTGTAAGAAT GCTGTTGTAA ACGACTCCCC TGAGACAGAA 
GCATGGCCCT CAGTCCCTGG CAGTGATCCA GAGTTGGCTT CAGAATGTAT GGATGCTGAT 
TCTGCCTCCA GTTCTGAATC AGAGAGAAAC ATCACTATCA TGGCTTCAGG GAGCACAGGT 
GGTGAAAAAG ATGGCCTTCG GAATAGCACT GGACTTGGTT CCCAAAACAA GTTTGTGGTT 
GGTAGCAGCA GCAATAATGT GGGCCATGGA AGTAGTCCTG GGCCATGGGG TTTTTCCCAT 
GGAGCCATAA TAAGCACATG TCAGGTCTCT GTGGATGCTC CTGAAAGCAA ATCAGAAAGT 
AGCAACAATA GAATGAATGC TTGGGGCACT GTAAGTTCTT CATCAAATGG AGGGTTAAAT 
CCAAGCACTT TGAATTCAGC TAGCAACCAT GGTGCCTGGC CAGTATTAGA CAACAACGGA 
CTTGCCCTCA AAGGGCCTGT AGGGAGCGGT AGTTCTGGCA TCAATATTCA GTGTAGTACC 
ATAGGCCAGA TGCCTAACAA TCAGAGTATT AACTCTAAAG TGGGTGGTTC TTCCACCCAT 
GGTACCTGGG GAAGCCTTCA GGAAACTTGT GAATCTGAAG TAAGTGGTAC ACAGAAGGTT 
TCATTCAGTG GTCAACCTCA GAATATTACC ACTGAGATGA CTGGACCAAA TAACACTACT 
AACTTTATGA CCTCTAGTTT ACCAAACTCC GGTTCAGTAC AGAATAACGA ACTGCCTAGT 
AATACAGGGG CCTGGCGTGT GAGCACAATG AATCATCCTC AGATACAGGC TCCATCGGTT 
ATGAATGGCA CTTCCCTTTC TCATCTTAGC AATGGAGAGC CAAAAAGTGG AGGCTCTTAC 
GGTACTACGT GGGGTGCCTA TGGTTCTAAT TACTCTGGAG ACAAATGTTC GAGCCCTAAC 
GGCCAAGCTA ATGGTGACAC TGTGAATGCA ACTCTAATGC AGCCTGGCAT AAATGGGCCT 
ATGGGCACTA ACTTTCAAGT GAACACAAAT AAAGGAGGAG GTGTGTGGGA GTCTGGGGCA 
ACGAATTCCC AGAATGCATC GTGGGGAAGT GGAAATGGCG CAAATTCTGG AGGAAGTCGA 
CGAGGATGGG GAACTCCTGC ACAAAACACT GGCACTAATA TACCCAGCGT GGAGTGGAGC 
AAACTGCCTA GCAATCAGCA TTCCAATGAC AGTGCAAATG GCAATGGTAA GAAGTTTACA 
AATGGATGGA AATCTACTGA GGAAGAGGAT CAGGCTTCTG CCGCATCTCA GACAACTGAG 
CAGAACAGTG TGTGGGCCAA AACAGGAGGT ACAGTGGAGA GTGAAGGTAG TACAGAAAGC 
ACTGGACGCC TCGAGGAGAA AGCCACTGGG GAAAATCAGA GTAGAGATCG GAGAAAAATT 
GATCAGCACA CATTACTCCA AAGCATCGTA AACAGAACTG ACTTGGATCC ACGTGTCCTC 
TCCAACTCTG GTTGGGGACA GACTCCTATT AAGCAGAATA CTGCCTGGGA TACGGAAACA 
TCACCTAGAG GGGAAAGAAA GACTGACAAT GGGACAGAGG CCTGGGGAAG CTCTGCAACA 
CAGACTTTTA ACTCAGGGGC ATGTACAGAT AAGACTAGCC CTACTAGTAA TGATACCTCA 
TCTGTATCGG GGTGGGGAGA TCCCAAACCT GCTCTGAGGT GGGGAGATTC CAAAGGCTCA 
AGCTGCCAGG GGGGATGGGA AGATGATGCT GCTGCTACAG GAATGGTCAA GAGCAATCAG 
TGGGGGAACA GCAAAGAAGA GAAGTCCGCA TGGAATGATT CGCAAAAGAG CAAACAGGGA 
TGGGGTGATG GACAGAAATC AAACCAAGGG TGGTCCGTTT CTGCCAGTGA TAACTGGGGA 
GAAACGTCAA GGAGTAACCA TTGGGGTGAG GCTAATAAGA AATCTAGCTC AGGAGGTAGT 
GACAGTGACA GGTCTGTTTC TGGTTGGAAT GAACTTGGTA AAACTAGTTC TTTTACTTGG 
GGAAATAACA TAAATCCAAA TAATTCGTCA GGATGGGATG AATCTTCTAA ACCTAATCCT 
TCCCAGGGAT GGGGAGATCC TCCAAAGTCT AATCAGTCTC TAGGTTGGGG AGATTCGTCA 
AAGCCAGTCA CTTCTCCAGA CTGGAACAAG CAACAAGACA TTGTCGGGTC TTGGGGAATC 
CCACCAGCAA CAGGCAAACC TCCTGGTACA GGCTGGCTGG GAGGACCTAT ACCAGCCCCA 
ACAAAAGAAG AAGAGCCCAC AGGCTGGGAG GAACCATCCC CAGAATCCAT ACGTCGCAAA 
ATGGAGATCG ATGACGGAAC TTCAGCTTGG GGAGATCCGA GCAAATACAA CTACAAAAAT 
GTGAACATGT GGAACAAAAA TGTCCCAAAT GGCAGCAGCC GTTCAGACCA GCAAGCACAG 
GTACACCAGT TGCTACCATC TGCAAGTGCC ATCTCAAACA AAGAGGCAGG CAGTGGCTCT 
GGCTGGGGTG AGCCCTGGGG GGAGCCTTCT ACTCCAGCCA CAACTGTGGA TAATGGTACT 
TCAGCATGGG GTAAACCCAT AGACAGTGGT CCCAGCTGGG GGGAGCCCAT TGCTGCAGCA 
TCCAACACAT CCACGTGGGG CTCCAGCTCT GTTGGTCCTC AAGCATTAAG CAAATCTGGG 
CCAAAATCTA TGCAAGATGG CTGGTGTGGT GATGATATGC CATTGCCTGG AAATCGCCCC 
ACTGGCTGGG AAGAGGAAGA GGATGTAGAG ATTGGAATGT GGAATAGTAA TTCATCTCAA 
GAGCTTAACT CATCTTTAAA TTGGCCTCCA TATACAAAGA AAATGTCATC GAAGGGTCTG 
AGTGGCAAAA AAAGGAGAAG GGAAAGGGGA ACCATGAAAG GTGGAAACAA ACAAGAAGAA 
GCATGGATAA ATCCATTCGT TAAACAGTTT TCAAATATCA GTTTTTCGAG AGACTCACCC 
GAAGAAAATG TACAGAGCAA TAAGATGGAT CTTTCCGGAG GAATGTTACA GGACAAACGA 
ATGGAGATAG ATAAACATAG CCTAAATATT GGTGATTACA ATCGAACGGT CGGGAAAGGC 
CCTGGTTCTC GGCCTCAGAT TTCCAAAGAG TCTTCCATGG AGCGCAGCCC TTACTTTGAT 
AAGGATGGCA TTGTAGCAGA TGAATCCCAA AACATGCAGT TTATGTCCAG TCAAAGCATG 
AAGCTTCCCC CTTCAAATAG TGCACTACCT AACCAGGCCC TTGGCTCCAT AGCAGGGCTG 
GGTATGCAAA ACTTGAATTC TGTTAGACAG AATGGCAATC CCAGTATGTT TGGTGTTGGA 
AACACAGCAG CACAACCCCG GGGCATGCAG CAGCCTCCAG CACAACCTCT TAGTTCATCT 
CAGCCTAATC TTCGTGCTCA AGTGCCTCCT CCATTACTCT CCCCTCAGGT TCCAGTTTCA 
TTGCTGAAGT ATGCACCAAA CAACGGTGGC CTGAATCCGC TCTTTGGCCC TCAACAGGTA 
GCCATGCTGA ACCAGCTATC CCAACTAAAC CAGCTTTCTC AGATCTCCCA GTTACAGAGA 
TTGTTAGCGC AGCAGCAAAG GGCGCAGAGT CAGAGAAGCG TGCCTTCTGG GAACCGGCAG 
CAGCAAGACC AGCAGGGTCG ACCTCTTAGC GTGCAGCAGC AGATGATGCA GCAGTCCCGG 
CAACTCGATC CCAGCCTGTT GGTGAAGCAG CAGACCCCGC CATCTCAGCA GCAGCCACTC 
CATCAGCCAG CCATGAAATC CTTCCTCGAG AATGTCATGC CCCACACTAC ACCTGAGCTG 
CAGAAAGGGC CATCACCAAT AAATGCTTTC AGCAACTTCC CGATAGGCTT GAACTCAAAC 
TTGAATGTAA ATATGGATAT GAACAGTATT AAAGAGCCAC AGTCAAGACT AAGGAAGTGG 
ACTACAGTGG ACAGCATTTC TGTGAACACA TCTTTGGATC AAAACTCCAG CAAACATGGT 
GCTATTTCAA GTGGTTTCAG GCTGGAAGAG TCTCCATTTG TTCCCTATGA CTTTATGAAC 
AGCAGTACTT CTCCAGCCAG CCCTCCGGGT TCAATAGGAG ATGGCTGGCC ACGTGCCAAA 
TCGCCTAACG GCTCTAGCAG TGTTAACTGG CCACCAGAAT TTCGCCCTGG TGAACCATGG 
AAAGGTTATC CAAACATTGA CCCTGAAACT GACCCTTACG TCACTCCTGG CAGTGTCATA 
AACAATCTTT CAATTAATAC TGTGCGGGAA GTTGACCACC TCAGGGACAG GAACAGTGGG 
TCATCCTCAT CCTTGAACAC CACGCTGCCT TCAACTAGTG CCTGGTCATC CATTCGTGCC 
TCCAACTACA ACGTTCCCCT CAGCAGTACA GCACAAAGCA CTTCAGCCAG AAATAGTGAT 
TCCAAATTGA CATGGTCTCC TGGTTCAGTT ACAAACACCT CTCTGGCTCA TGAGCTGTGG 
AAGGTCCCTT TACCACCTAA AAACATCACT GCTCCGTCCC GCCCACCTCC GGGACTCACT 
GGTCAGAAGC CACCCTTGTC TACGTGGGAT AACTCTCCCC TTCGTGTAGG TGGAGGATGG 
GGAAATTCTG ACGCCAGGTA CACCCCAGGT TCCAGTTGGG GTGAGAGCAG CTCAGGGAGA 
ATAACAAATT GGCTTGTTCT AAAGAACCTC ACACCTCAGA TTGACGGCTC AACTCTGCGT 
ACTCTGTGCA TGCAGCATGG CCCACTGATA ACATTCCACC TGAACCTCCC ACATGGAAAT 
GCTTTGGTCC GTTACAGTTC AAAAGAAGAG GTAGTGAAGG CACAAAAGTC TCTGCACATG 
TGTGTATTGG GGAACACTAC TATTCTTGCT GAGTTTGCCA GTGAAGAGGA GATCAGCCGT 
TTCTTTGCAC AAAGTCAGTC TCTGACCCCT TCTCCCGGCT GGCAGTCTCT CGGGTCCAGC 
CAGAGCCGGC TGGGCTCCCT CGACTGTTCC CACTCGTTCT CCAGCCGGAC CGATCTCAAT 
CACTGGAATG GTGCTGGGCT GTCGGGAACT AACTGTGGAG ACCTTCACGG CACTTCACTC 
TGGGGGACCC CACATTATTC CACAAGCCTG TGGGGTCCTC CCAGCAGCAG CGACCCCCGG 
GGAATTAGCA GCCCATCCCC CATTAATGCT TTTCTTTCTG TTGACCACCT GGGTGGGGGT 
GGAGAGTCCA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970011.1
CDS	282..5816
Translation	MQLVAEPGLEARCPGSQDIPLPEEWNRELEAKATKDVERTLSRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKDINHSTSGSHYENSQRGPVSSPSDSSTNCKNAVVNDSPETEAWPSVPGSDPELASECMDADSASSSESERNITIMASGSTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSPGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLDNNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVGGSSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVMNGTSLSHLSNGEPKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGINGPMGTNFQVNTNKGGGVWESGATNSQNASWGSGNGANSGGSRRGWGTPAQNTGTNIPSVEWSKLPSNQHSNDSANGNGKKFTNGWKSTEEEDQASAASQTTEQNSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACTDKTSPTSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDAAATGMVKSNQWGNSKEEKSAWNDSQKSKQGWGDGQKSNQGWSVSASDNWGETSRSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVTSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPTKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSASAISNKEAGSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASNTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGTMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERSPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPSLLVKQQTPPSQQQPLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_014114536.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6A (TNRC6A), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014114536.1

ORF Insert Sequence:

ATGCAACTAG TGGCAGAGCC AGGCCTAGAA GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG AGAATTGGAA GCTAAAGCTA CCAAAGACGT AGAAAGGACT 
CTTAGCAGGG ATTTAGTGCA AGAAGAAGAA CAGTTGATGG AAGAAAAGAA AAAGAAAAAA 
GACGACAAGA AAAAGAAGGA AGCTGCTCAA AAGAAGGCCA CTGAACAAAA AATCAAAGAT 
ATAAACCACA GTACTTCAGG ATCCCATTAT GAAAATTCAC AGCGGGGACC TGTGTCTTCT 
CCGAGTGACT CTAGCACAAA TTGTAAGAAT GCTGTTGTAA ACGACTCCCC TGAGACAGAA 
GCATGGCCCT CAGTCCCTGG CAGTGATCCA GAGTTGGCTT CAGAATGTAT GGATGCTGAT 
TCTGCCTCCA GTTCTGAATC AGAGAGAAAC ATCACTATCA TGGCTTCAGG GAGCACAGGT 
GGTGAAAAAG ATGGCCTTCG GAATAGCACT GGACTTGGTT CCCAAAACAA GTTTGTGGTT 
GGTAGCAGCA GCAATAATGT GGGCCATGGA AGTAGTCCTG GGCCATGGGG TTTTTCCCAT 
GGAGCCATAA TAAGCACATG TCAGGTCTCT GTGGATGCTC CTGAAAGCAA ATCAGAAAGT 
AGCAACAATA GAATGAATGC TTGGGGCACT GTAAGTTCTT CATCAAATGG AGGGTTAAAT 
CCAAGCACTT TGAATTCAGC TAGCAACCAT GGTGCCTGGC CAGTATTAGA CAACAACGGA 
CTTGCCCTCA AAGGGCCTGT AGGGAGCGGT AGTTCTGGCA TCAATATTCA GTGTAGTACC 
ATAGGCCAGA TGCCTAACAA TCAGAGTATT AACTCTAAAG TGGGTGGTTC TTCCACCCAT 
GGTACCTGGG GAAGCCTTCA GGAAACTTGT GAATCTGAAG TAAGTGGTAC ACAGAAGGTT 
TCATTCAGTG GTCAACCTCA GAATATTACC ACTGAGATGA CTGGACCAAA TAACACTACT 
AACTTTATGA CCTCTAGTTT ACCAAACTCC GGTTCAGTAC AGAATAACGA ACTGCCTAGT 
AATACAGGGG CCTGGCGTGT GAGCACAATG AATCATCCTC AGATACAGGC TCCATCGGTT 
ATGAATGGCA CTTCCCTTTC TCATCTTAGC AATGGAGAGC CAAAAAGTGG AGGCTCTTAC 
GGTACTACGT GGGGTGCCTA TGGTTCTAAT TACTCTGGAG ACAAATGTTC GAGCCCTAAC 
GGCCAAGCTA ATGGTGACAC TGTGAATGCA ACTCTAATGC AGCCTGGCAT AAATGGGCCT 
ATGGGCACTA ACTTTCAAGT GAACACAAAT AAAGGAGGAG GTGTGTGGGA GTCTGGGGCA 
ACGAATTCCC AGAATGCATC GTGGGGAAGT GGAAATGGCG CAAATTCTGG AGGAAGTCGA 
CGAGGATGGG GAACTCCTGC ACAAAACACT GGCACTAATA TACCCAGCGT GGAGTGGAGC 
AAACTGCCTA GCAATCAGCA TTCCAATGAC AGTGCAAATG GCAATGGTAA GAAGTTTACA 
AATGGATGGA AATCTACTGA GGAAGAGGAT CAGGCTTCTG CCGCATCTCA GACAACTGAG 
CAGAACAGTG TGTGGGCCAA AACAGGAGGT ACAGTGGAGA GTGAAGGTAG TACAGAAAGC 
ACTGGACGCC TCGAGGAGAA AGCCACTGGG GAAAATCAGA GTAGAGATCG GAGAAAAATT 
GATCAGCACA CATTACTCCA AAGCATCGTA AACAGAACTG ACTTGGATCC ACGTGTCCTC 
TCCAACTCTG GTTGGGGACA GACTCCTATT AAGCAGAATA CTGCCTGGGA TACGGAAACA 
TCACCTAGAG GGGAAAGAAA GACTGACAAT GGGACAGAGG CCTGGGGAAG CTCTGCAACA 
CAGACTTTTA ACTCAGGGGC ATGTACAGAT AAGACTAGCC CTACTAGTAA TGATACCTCA 
TCTGTATCGG GGTGGGGAGA TCCCAAACCT GCTCTGAGGT GGGGAGATTC CAAAGGCTCA 
AGCTGCCAGG GGGGATGGGA AGATGATGCT GCTGCTACAG GAATGGTCAA GAGCAATCAG 
TGGGGGAACA GCAAAGAAGA GAAGTCCGCA TGGAATGATT CGCAAAAGAG CAAACAGGGA 
TGGGGTGATG GACAGAAATC AAACCAAGGG TGGTCCGTTT CTGCCAGTGA TAACTGGGGA 
GAAACGTCAA GGAGTAACCA TTGGGGTGAG GCTAATAAGA AATCTAGCTC AGGAGGTAGT 
GACAGTGACA GGTCTGTTTC TGGTTGGAAT GAACTTGGTA AAACTAGTTC TTTTACTTGG 
GGAAATAACA TAAATCCAAA TAATTCGTCA GGATGGGATG AATCTTCTAA ACCTAATCCT 
TCCCAGGGAT GGGGAGATCC TCCAAAGTCT AATCAGTCTC TAGGTTGGGG AGATTCGTCA 
AAGCCAGTCA CTTCTCCAGA CTGGAACAAG CAACAAGACA TTGTCGGGTC TTGGGGAATC 
CCACCAGCAA CAGGCAAACC TCCTGGTACA GGCTGGCTGG GAGGACCTAT ACCAGCCCCA 
ACAAAAGAAG AAGAGCCCAC AGGCTGGGAG GAACCATCCC CAGAATCCAT ACGTCGCAAA 
ATGGAGATCG ATGACGGAAC TTCAGCTTGG GGAGATCCGA GCAAATACAA CTACAAAAAT 
GTGAACATGT GGAACAAAAA TGTCCCAAAT GGCAGCAGCC GTTCAGACCA GCAAGCACAG 
GTACACCAGT TGCTACCATC TGCAAGTGCC ATCTCAAACA AAGAGGCAGG CAGTGGCTCT 
GGCTGGGGTG AGCCCTGGGG GGAGCCTTCT ACTCCAGCCA CAACTGTGGA TAATGGTACT 
TCAGCATGGG GTAAACCCAT AGACAGTGGT CCCAGCTGGG GGGAGCCCAT TGCTGCAGCA 
TCCAACACAT CCACGTGGGG CTCCAGCTCT GTTGGTCCTC AAGCATTAAG CAAATCTGGG 
CCAAAATCTA TGCAAGATGG CTGGTGTGGT GATGATATGC CATTGCCTGG AAATCGCCCC 
ACTGGCTGGG AAGAGGAAGA GGATGTAGAG ATTGGAATGT GGAATAGTAA TTCATCTCAA 
GAGCTTAACT CATCTTTAAA TTGGCCTCCA TATACAAAGA AAATGTCATC GAAGGGTCTG 
AGTGGCAAAA AAAGGAGAAG GGAAAGGGGA ACCATGAAAG GTGGAAACAA ACAAGAAGAA 
GCATGGATAA ATCCATTCGT TAAACAGTTT TCAAATATCA GTTTTTCGAG AGACTCACCC 
GAAGAAAATG TACAGAGCAA TAAGATGGAT CTTTCCGGAG GAATGTTACA GGACAAACGA 
ATGGAGATAG ATAAACATAG CCTAAATATT GGTGATTACA ATCGAACGGT CGGGAAAGGC 
CCTGGTTCTC GGCCTCAGAT TTCCAAAGAG TCTTCCATGG AGCGCAGCCC TTACTTTGAT 
AAGGATGGCA TTGTAGCAGA TGAATCCCAA AACATGCAGT TTATGTCCAG TCAAAGCATG 
AAGCTTCCCC CTTCAAATAG TGCACTACCT AACCAGGCCC TTGGCTCCAT AGCAGGGCTG 
GGTATGCAAA ACTTGAATTC TGTTAGACAG AATGGCAATC CCAGTATGTT TGGTGTTGGA 
AACACAGCAG CACAACCCCG GGGCATGCAG CAGCCTCCAG CACAACCTCT TAGTTCATCT 
CAGCCTAATC TTCGTGCTCA AGTGCCTCCT CCATTACTCT CCCCTCAGGT TCCAGTTTCA 
TTGCTGAAGT ATGCACCAAA CAACGGTGGC CTGAATCCGC TCTTTGGCCC TCAACAGGTA 
GCCATGCTGA ACCAGCTATC CCAACTAAAC CAGCTTTCTC AGATCTCCCA GTTACAGAGA 
TTGTTAGCGC AGCAGCAAAG GGCGCAGAGT CAGAGAAGCG TGCCTTCTGG GAACCGGCAG 
CAGCAAGACC AGCAGGGTCG ACCTCTTAGC GTGCAGCAGC AGATGATGCA GCAGTCCCGG 
CAACTCGATC CCAGCCTGTT GGTGAAGCAG CAGACCCCGC CATCTCAGCA GCAGCCACTC 
CATCAGCCAG CCATGAAATC CTTCCTCGAG AATGTCATGC CCCACACTAC ACCTGAGCTG 
CAGAAAGGGC CATCACCAAT AAATGCTTTC AGCAACTTCC CGATAGGCTT GAACTCAAAC 
TTGAATGTAA ATATGGATAT GAACAGTATT AAAGAGCCAC AGTCAAGACT AAGGAAGTGG 
ACTACAGTGG ACAGCATTTC TGTGAACACA TCTTTGGATC AAAACTCCAG CAAACATGGT 
GCTATTTCAA GTGGTTTCAG GCTGGAAGAG TCTCCATTTG TTCCCTATGA CTTTATGAAC 
AGCAGTACTT CTCCAGCCAG CCCTCCGGGT TCAATAGGAG ATGGCTGGCC ACGTGCCAAA 
TCGCCTAACG GCTCTAGCAG TGTTAACTGG CCACCAGAAT TTCGCCCTGG TGAACCATGG 
AAAGGTTATC CAAACATTGA CCCTGAAACT GACCCTTACG TCACTCCTGG CAGTGTCATA 
AACAATCTTT CAATTAATAC TGTGCGGGAA GTTGACCACC TCAGGGACAG GAACAGTGGG 
TCATCCTCAT CCTTGAACAC CACGCTGCCT TCAACTAGTG CCTGGTCATC CATTCGTGCC 
TCCAACTACA ACGTTCCCCT CAGCAGTACA GCACAAAGCA CTTCAGCCAG AAATAGTGAT 
TCCAAATTGA CATGGTCTCC TGGTTCAGTT ACAAACACCT CTCTGGCTCA TGAGCTGTGG 
AAGGTCCCTT TACCACCTAA AAACATCACT GCTCCGTCCC GCCCACCTCC GGGACTCACT 
GGTCAGAAGC CACCCTTGTC TACGTGGGAT AACTCTCCCC TTCGTGTAGG TGGAGGATGG 
GGAAATTCTG ACGCCAGGTA CACCCCAGGT TCCAGTTGGG GTGAGAGCAG CTCAGGGAGA 
ATAACAAATT GGCTTGTTCT AAAGAACCTC ACACCTCAGA TTGACGGCTC AACTCTGCGT 
ACTCTGTGCA TGCAGCATGG CCCACTGATA ACATTCCACC TGAACCTCCC ACATGGAAAT 
GCTTTGGTCC GTTACAGTTC AAAAGAAGAG GTAGTGAAGG CACAAAAGTC TCTGCACATG 
TGTGTATTGG GGAACACTAC TATTCTTGCT GAGTTTGCCA GTGAAGAGGA GATCAGCCGT 
TTCTTTGCAC AAAGTCAGTC TCTGACCCCT TCTCCCGGCT GGCAGTCTCT CGGGTCCAGC 
CAGAGCCGGC TGGGCTCCCT CGACTGTTCC CACTCGTTCT CCAGCCGGAC CGATCTCAAT 
CACTGGAATG GTGCTGGGCT GTCGGGAACT AACTGTGGAG ACCTTCACGG CACTTCACTC 
TGGGGGACCC CACATTATTC CACAAGCCTG TGGGGTCCTC CCAGCAGCAG CGACCCCCGG 
GGAATTAGCA GCCCATCCCC CATTAATGCT TTTCTTTCTG TTGACCACCT GGGTGGGGGT 
GGAGAGTCCA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TNRC6A
Entrez Gene ID	489966
Full Name	trinucleotide repeat containing 6A
General protein information	Preferred Names trinucleotide repeat containing 6A Names trinucleotide repeat-containing gene 6A protein
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Homo sapiens (human)	TNRC6A	NP_055309.2
	TNRC6A	XP_001098013.2
Bos taurus (cattle)	TNRC6A	XP_005224864.1
Gallus gallus (chicken)	TNRC6A	XP_414871.4
Xenopus tropicalis (tropical clawed frog)	tnrc6a	XP_002932511.2
Canis lupus familiaris (dog)	TNRC6A	XP_005621456.1
Mus musculus (house mouse)	Tnrc6a	NP_659174.3
Rattus norvegicus (Norway rat)	Tnrc6a	NP_001101019.1
Pan troglodytes (chimpanzee)	TNRC6A	XP_510885.3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TNRC6A cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

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ORF Insert Sequence:

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