ECE2 cDNA ORF clone, Canis lupus familiaris(dog)

The following ECE2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ECE2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe284461 XM_545229.6
Latest version!
Canis lupus familiaris endothelin converting enzyme 2 (ECE2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00
OCe30452 XM_854235.4
Latest version!
Canis lupus familiaris endothelin converting enzyme 2 (ECE2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe30455 XM_003434114.3
Latest version!
Canis lupus familiaris endothelin converting enzyme 2 (ECE2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe44828 XM_014110394.2
Latest version!
Canis lupus familiaris endothelin converting enzyme 2 (ECE2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OCe44829 XM_022414235.1
Latest version!
Canis lupus familiaris endothelin converting enzyme 2 (ECE2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OCe44829 XM_014110395.1
Latest version!
Canis lupus familiaris endothelin converting enzyme 2 (ECE2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OCe44830 XM_014110396.2
Latest version!
Canis lupus familiaris endothelin converting enzyme 2 (ECE2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OCe44831 XM_005639769.3
Latest version!
Canis lupus familiaris endothelin converting enzyme 2 (ECE2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe30457 XM_545229.5
Latest version!
Canis lupus familiaris endothelin converting enzyme 2 (ECE2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $174.30
$249.00
View Old Accession Versions >>
OCe44831 XM_005639769.2 Canis lupus familiaris endothelin converting enzyme 2 (ECE2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe44828 XM_014110394.1 Canis lupus familiaris endothelin converting enzyme 2 (ECE2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OCe30452 XM_854235.3 Canis lupus familiaris endothelin converting enzyme 2 (ECE2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe44830 XM_014110396.1 Canis lupus familiaris endothelin converting enzyme 2 (ECE2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OCe30455 XM_003434114.2 Canis lupus familiaris endothelin converting enzyme 2 (ECE2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe284461
    Clone ID Related Accession (Same CDS sequence) XM_545229.6
    Accession Version XM_545229.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 768bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product endothelin-converting enzyme 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_545229.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGGCCTCTC CGAGGGCCCC GGCCCCGGCG CGGGAGATAC CGGAGCAGAA CTGCTGGTAC 
    CGTGAGGTCC AGTACTGGGA CCGGCGCTAC CGGAATGCCG CGGACTCTGC CCCTTACGAG
    TGGTTCGGAG ACTTCTCCTC CTTCCGTGCC CTCCTGGAGC CTGAGCTGCA GCCAGAAGAC
    CGTATCCTCG TGCTAGGCTG TGGGAACAGC GCGCTGAGCT ACGAGCTGTT CCTTGGGGGC
    TTCCCTGATG TCACCAGTGT GGACTACTCA TCAGTAGTGG TGGCTGCTAT GCAGGCTCGC
    TATGCCCACG TACCCACGCT GCGCTGGGAG ACCATGGATG TACGGGCACT GGACTTCCCC
    AGTGGCTCCT TCGACGTGGT GCTGGAGAAG GGCACGCTGG ATGCCCTGTT AGCTGGAGAA
    CAGGACCCCT GGAATGTGTC TTCTGAAGGT GTCCACACCA TGGATCAGGT GCTGAGTGAG
    GTGAGCCGGG TGCTGGTCCA TGGAGGCCGG TTCATCTCAT TGACCTCAGC TGCTCCCCAC
    TTTCGGATCA GACACTATGC CCAAGCCCGA TATGGCTGGT CCCTGAGGCA TGCAACCTAT
    GGCAGTGGTT TCCACTTCCA TCTTTACATC ATGCAGAAGG GCAAAGAGCT CACTGTGGCC
    CAGCTGGCCC TTGGGACCAA GATCCTTTCA CCCCCTAGAC CTCCCACCTC GCCCTGCTTC
    CTCCAGGACT CGGATCATGA GGACTTCCTC AGTGCCATTC AGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_545229.3
    CDS84..851
    Translation

    Target ORF information:

    RefSeq Version XM_545229.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris endothelin converting enzyme 2 (ECE2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_545229.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGGCCTCTC CGAGGGCCCC GGCCCCGGCG CGGGAGATAC CGGAGCAGAA CTGCTGGTAC 
    CGTGAGGTCC AGTACTGGGA CCGGCGCTAC CGGAATGCCG CGGACTCTGC CCCTTACGAG
    TGGTTCGGAG ACTTCTCCTC CTTCCGTGCC CTCCTGGAGC CTGAGCTGCA GCCAGAAGAC
    CGTATCCTCG TGCTAGGCTG TGGGAACAGC GCGCTGAGCT ACGAGCTGTT CCTTGGGGGC
    TTCCCTGATG TCACCAGTGT GGACTACTCA TCAGTAGTGG TGGCTGCTAT GCAGGCTCGC
    TATGCCCACG TACCCACGCT GCGCTGGGAG ACCATGGATG TACGGGCACT GGACTTCCCC
    AGTGGCTCCT TCGACGTGGT GCTGGAGAAG GGCACGCTGG ATGCCCTGTT AGCTGGAGAA
    CAGGACCCCT GGAATGTGTC TTCTGAAGGT GTCCACACCA TGGATCAGGT GCTGAGTGAG
    GTGAGCCGGG TGCTGGTCCA TGGAGGCCGG TTCATCTCAT TGACCTCAGC TGCTCCCCAC
    TTTCGGATCA GACACTATGC CCAAGCCCGA TATGGCTGGT CCCTGAGGCA TGCAACCTAT
    GGCAGTGGTT TCCACTTCCA TCTTTACATC ATGCAGAAGG GCAAAGAGCT CACTGTGGCC
    CAGCTGGCCC TTGGGACCAA GATCCTTTCA CCCCCTAGAC CTCCCACCTC GCCCTGCTTC
    CTCCAGGACT CGGATCATGA GGACTTCCTC AGTGCCATTC AGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30452
    Clone ID Related Accession (Same CDS sequence) XM_854235.4 , XM_854235.3
    Accession Version XM_854235.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2298bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product endothelin-converting enzyme 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_854235.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAACGTCG CGCTGCAGGA GCTGGGCGGC GGCGGCAACA TGGTGGAGTA CGAGCGGACC 
    ACGCTGCGGG AGGAAGACGC ACCTGAGACC CCCGTAGAGG GCGGGGCCTC CCCGGACGCT
    GTGGAGGTGG GATTCCGGAA GAGGACAAGA GACTTCTTGG GCTCGCACAC CCAACTGGAG
    CTGGTCTTAG CCGCTGTCTC TCTACTGCTG GCTGCACTGC TACTGGGCTG CTTGGTGGCT
    CTGGGGGTCC AGTACCACGG GGACCCATCC CACAACACCT GCCTCACAGA GGCCTGCATT
    CGAGTAGCTG GAAAGATTCT GGAGTCCCTG GACCGCGGGG TGAGTCCCTG TGAGGACTTT
    TACCAGTTTT CCTGTGGTGG CTGGATTCAG AGAAATCCTC TGCCCGATGG GCGTTCTCGT
    TGGAACACCT TCAACAGCCT CTGGGACCAA AATCAGGCCA TACTGAAGCA CCTGCTTGAA
    AACACCACCT TCAACTCTAC CAGTGAAGCT GAGCGGAAGA CACAGCGTTT CTACCTCTCC
    TGCCTGCAGG TGGAGCGGAT CGAGGAACTG GGAGCCCAGC CACTGCGAGA CCTCATTGAC
    AAGATTGGTG GTTGGAACAT TACCGGACCC TGGGACCAGG ACAACTTCAT GGAAGTGCTG
    AAGGCAGTAG CAGGGACCTA CAGAGCTACT CCCTTCTTCA CTGTCTACGT CAGTGCCGAC
    TCTAAGAGCT CTAACAGCAA TGTTATCCAG GTGGACCAAT CTGGGCTCTT TCTCCCCTCT
    CGAGACTACT ACCTAAACAG GACCGCCAAT GAAAAAGTGC TTACCGCCTA CCTTGACTAC
    ATGGAGGAGC TGGGGATGTT GCTGGGTGGA CGGCCAACCT CCACACGGGA GCAGATGCGG
    CAGGTGCTGG AGCTGGAGAT ACAACTGGCC AACATCACAG TGCCCCAGGA CCAGAGGCGC
    GATGAGGAGA AGATCTATCA TAAGATGAAC ATTGCAGAGC TGCAGGCCCT GGCACCCTCT
    GTGGACTGGC TGGAGTTTCT GTCTTTCTTG TTGTCACCGC TGGAGCTGGG TGACTCTGAA
    CCTGTGGTGG TGTATGGAAC GGATTATTTG CAGCAGGTGT CAGAGCTCAT CAACCACACA
    GAGCCAAGTG TCCTGAACAA TTACCTGATT TGGAATCTGG TACAGAAGAC AACCTCAAGC
    CTGGACCACC GCTTTGAATC TGCACAGGAG AAACTGCTGG AGACTCTCTA TGGCACCAAG
    AAGTCCTGCA TGCCAAGGTG GCAGACCTGC ATCTCCAACA CGGACGACGC ACTTGGCTTC
    GCTTTGGGCT CCCTCTTTGT GAAGGCCACG TTCGATCGGC AAAGCAAGGA AATTGCTGAA
    GGGATGATCA GCGAGATCCG GAATGCCTTT GAGGAAGCCC TGGGGCAGCT GGTTTGGATG
    GATGAGAAGA CCCGCCAGGC AGCCAAGGAG AAAGCAGATG CTATATATGA TATGATTGGT
    TTCCCGGACT TCATCCTGGA TCCCAAAGAG CTGGATGATG TTTATGATGG GTACGAAGTC
    TCTGAAGATT CCTTCTTCCA GAACATGTTG AATTTGTACA ATTTCTCTGC TAAGGTGATG
    GCTGACCAGC TCCGCAAGCC TCCCAGCCGG GACCAGTGGA GCATGACCCC CCAGACAGTG
    AATGCCTACT ACCTTCCAAC CAAGAATGAA ATTGTCTTCC CTGCCGGCAT CTTGCAGGCC
    CCCTTCTACA CTCGTAACCA CCCCAAGGCC CTGAACTTTG GTGGAATCGG CGTGGTGATG
    GGCCATGAGT TGACACATGC GTTTGATGAC CAAGGTCGCG AATATGACAA GGAAGGGAAC
    CTGCGGCCCT GGTGGCAGAA TGAGTCCCTG GCAGCCTTCC GGAATCACAC AGCTTGCATG
    GAGGAACAGT ACAGCCAGTA CCAGGTCAAC GGGGAGAGGC TCAATGGGCG CCAGACGCTG
    GGGGAGAACA TCGCTGACAA CGGGGGGCTG AAGGCTGCCT ATAACGCTTA CAAAGCATGG
    CTGAGCAAGC ATGGGGAAGA GCAGCAGCTG CCAGCCGTGG GGCTCACCAA TCACCAGCTC
    TTCTTTGTGG GATTTGCCCA GGTGTGGTGC TCAGTCCGCA CACCCGAGAG CTCTCACGAG
    GGGCTGGTGA CTGACCCCCA CAGCCCCGCC CGCTTCCGCG TGCTGGGCAC TCTCTCCAAC
    TCCCGGGACT TCCTGCGACA CTTCGGCTGC CCTGTCGGCT CCCCCATGAA CCCAGGGCAG
    CTGTGTGAGG TGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_859328.1
    CDS90..2387
    Translation

    Target ORF information:

    RefSeq Version XM_854235.4
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris endothelin converting enzyme 2 (ECE2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_854235.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAACGTCG CGCTGCAGGA GCTGGGCGGC GGCGGCAACA TGGTGGAGTA CGAGCGGACC 
    ACGCTGCGGG AGGAAGACGC ACCTGAGACC CCCGTAGAGG GCGGGGCCTC CCCGGACGCT
    GTGGAGGTGG GATTCCGGAA GAGGACAAGA GACTTCTTGG GCTCGCACAC CCAACTGGAG
    CTGGTCTTAG CCGCTGTCTC TCTACTGCTG GCTGCACTGC TACTGGGCTG CTTGGTGGCT
    CTGGGGGTCC AGTACCACGG GGACCCATCC CACAACACCT GCCTCACAGA GGCCTGCATT
    CGAGTAGCTG GAAAGATTCT GGAGTCCCTG GACCGCGGGG TGAGTCCCTG TGAGGACTTT
    TACCAGTTTT CCTGTGGTGG CTGGATTCAG AGAAATCCTC TGCCCGATGG GCGTTCTCGT
    TGGAACACCT TCAACAGCCT CTGGGACCAA AATCAGGCCA TACTGAAGCA CCTGCTTGAA
    AACACCACCT TCAACTCTAC CAGTGAAGCT GAGCGGAAGA CACAGCGTTT CTACCTCTCC
    TGCCTGCAGG TGGAGCGGAT CGAGGAACTG GGAGCCCAGC CACTGCGAGA CCTCATTGAC
    AAGATTGGTG GTTGGAACAT TACCGGACCC TGGGACCAGG ACAACTTCAT GGAAGTGCTG
    AAGGCAGTAG CAGGGACCTA CAGAGCTACT CCCTTCTTCA CTGTCTACGT CAGTGCCGAC
    TCTAAGAGCT CTAACAGCAA TGTTATCCAG GTGGACCAAT CTGGGCTCTT TCTCCCCTCT
    CGAGACTACT ACCTAAACAG GACCGCCAAT GAAAAAGTGC TTACCGCCTA CCTTGACTAC
    ATGGAGGAGC TGGGGATGTT GCTGGGTGGA CGGCCAACCT CCACACGGGA GCAGATGCGG
    CAGGTGCTGG AGCTGGAGAT ACAACTGGCC AACATCACAG TGCCCCAGGA CCAGAGGCGC
    GATGAGGAGA AGATCTATCA TAAGATGAAC ATTGCAGAGC TGCAGGCCCT GGCACCCTCT
    GTGGACTGGC TGGAGTTTCT GTCTTTCTTG TTGTCACCGC TGGAGCTGGG TGACTCTGAA
    CCTGTGGTGG TGTATGGAAC GGATTATTTG CAGCAGGTGT CAGAGCTCAT CAACCACACA
    GAGCCAAGTG TCCTGAACAA TTACCTGATT TGGAATCTGG TACAGAAGAC AACCTCAAGC
    CTGGACCACC GCTTTGAATC TGCACAGGAG AAACTGCTGG AGACTCTCTA TGGCACCAAG
    AAGTCCTGCA TGCCAAGGTG GCAGACCTGC ATCTCCAACA CGGACGACGC ACTTGGCTTC
    GCTTTGGGCT CCCTCTTTGT GAAGGCCACG TTCGATCGGC AAAGCAAGGA AATTGCTGAA
    GGGATGATCA GCGAGATCCG GAATGCCTTT GAGGAAGCCC TGGGGCAGCT GGTTTGGATG
    GATGAGAAGA CCCGCCAGGC AGCCAAGGAG AAAGCAGATG CTATATATGA TATGATTGGT
    TTCCCGGACT TCATCCTGGA TCCCAAAGAG CTGGATGATG TTTATGATGG GTACGAAGTC
    TCTGAAGATT CCTTCTTCCA GAACATGTTG AATTTGTACA ATTTCTCTGC TAAGGTGATG
    GCTGACCAGC TCCGCAAGCC TCCCAGCCGG GACCAGTGGA GCATGACCCC CCAGACAGTG
    AATGCCTACT ACCTTCCAAC CAAGAATGAA ATTGTCTTCC CTGCCGGCAT CTTGCAGGCC
    CCCTTCTACA CTCGTAACCA CCCCAAGGCC CTGAACTTTG GTGGAATCGG CGTGGTGATG
    GGCCATGAGT TGACACATGC GTTTGATGAC CAAGGTCGCG AATATGACAA GGAAGGGAAC
    CTGCGGCCCT GGTGGCAGAA TGAGTCCCTG GCAGCCTTCC GGAATCACAC AGCTTGCATG
    GAGGAACAGT ACAGCCAGTA CCAGGTCAAC GGGGAGAGGC TCAATGGGCG CCAGACGCTG
    GGGGAGAACA TCGCTGACAA CGGGGGGCTG AAGGCTGCCT ATAACGCTTA CAAAGCATGG
    CTGAGCAAGC ATGGGGAAGA GCAGCAGCTG CCAGCCGTGG GGCTCACCAA TCACCAGCTC
    TTCTTTGTGG GATTTGCCCA GGTGTGGTGC TCAGTCCGCA CACCCGAGAG CTCTCACGAG
    GGGCTGGTGA CTGACCCCCA CAGCCCCGCC CGCTTCCGCG TGCTGGGCAC TCTCTCCAAC
    TCCCGGGACT TCCTGCGACA CTTCGGCTGC CCTGTCGGCT CCCCCATGAA CCCAGGGCAG
    CTGTGTGAGG TGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30452
    Clone ID Related Accession (Same CDS sequence) XM_854235.4 , XM_854235.3
    Accession Version XM_854235.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2298bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product endothelin-converting enzyme 2 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726120.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2013 this sequence version replaced XM_854235.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAACGTCG CGCTGCAGGA GCTGGGCGGC GGCGGCAACA TGGTGGAGTA CGAGCGGACC 
    ACGCTGCGGG AGGAAGACGC ACCTGAGACC CCCGTAGAGG GCGGGGCCTC CCCGGACGCT
    GTGGAGGTGG GATTCCGGAA GAGGACAAGA GACTTCTTGG GCTCGCACAC CCAACTGGAG
    CTGGTCTTAG CCGCTGTCTC TCTACTGCTG GCTGCACTGC TACTGGGCTG CTTGGTGGCT
    CTGGGGGTCC AGTACCACGG GGACCCATCC CACAACACCT GCCTCACAGA GGCCTGCATT
    CGAGTAGCTG GAAAGATTCT GGAGTCCCTG GACCGCGGGG TGAGTCCCTG TGAGGACTTT
    TACCAGTTTT CCTGTGGTGG CTGGATTCAG AGAAATCCTC TGCCCGATGG GCGTTCTCGT
    TGGAACACCT TCAACAGCCT CTGGGACCAA AATCAGGCCA TACTGAAGCA CCTGCTTGAA
    AACACCACCT TCAACTCTAC CAGTGAAGCT GAGCGGAAGA CACAGCGTTT CTACCTCTCC
    TGCCTGCAGG TGGAGCGGAT CGAGGAACTG GGAGCCCAGC CACTGCGAGA CCTCATTGAC
    AAGATTGGTG GTTGGAACAT TACCGGACCC TGGGACCAGG ACAACTTCAT GGAAGTGCTG
    AAGGCAGTAG CAGGGACCTA CAGAGCTACT CCCTTCTTCA CTGTCTACGT CAGTGCCGAC
    TCTAAGAGCT CTAACAGCAA TGTTATCCAG GTGGACCAAT CTGGGCTCTT TCTCCCCTCT
    CGAGACTACT ACCTAAACAG GACCGCCAAT GAAAAAGTGC TTACCGCCTA CCTTGACTAC
    ATGGAGGAGC TGGGGATGTT GCTGGGTGGA CGGCCAACCT CCACACGGGA GCAGATGCGG
    CAGGTGCTGG AGCTGGAGAT ACAACTGGCC AACATCACAG TGCCCCAGGA CCAGAGGCGC
    GATGAGGAGA AGATCTATCA TAAGATGAAC ATTGCAGAGC TGCAGGCCCT GGCACCCTCT
    GTGGACTGGC TGGAGTTTCT GTCTTTCTTG TTGTCACCGC TGGAGCTGGG TGACTCTGAA
    CCTGTGGTGG TGTATGGAAC GGATTATTTG CAGCAGGTGT CAGAGCTCAT CAACCACACA
    GAGCCAAGTG TCCTGAACAA TTACCTGATT TGGAATCTGG TACAGAAGAC AACCTCAAGC
    CTGGACCACC GCTTTGAATC TGCACAGGAG AAACTGCTGG AGACTCTCTA TGGCACCAAG
    AAGTCCTGCA TGCCAAGGTG GCAGACCTGC ATCTCCAACA CGGACGACGC ACTTGGCTTC
    GCTTTGGGCT CCCTCTTTGT GAAGGCCACG TTCGATCGGC AAAGCAAGGA AATTGCTGAA
    GGGATGATCA GCGAGATCCG GAATGCCTTT GAGGAAGCCC TGGGGCAGCT GGTTTGGATG
    GATGAGAAGA CCCGCCAGGC AGCCAAGGAG AAAGCAGATG CTATATATGA TATGATTGGT
    TTCCCGGACT TCATCCTGGA TCCCAAAGAG CTGGATGATG TTTATGATGG GTACGAAGTC
    TCTGAAGATT CCTTCTTCCA GAACATGTTG AATTTGTACA ATTTCTCTGC TAAGGTGATG
    GCTGACCAGC TCCGCAAGCC TCCCAGCCGG GACCAGTGGA GCATGACCCC CCAGACAGTG
    AATGCCTACT ACCTTCCAAC CAAGAATGAA ATTGTCTTCC CTGCCGGCAT CTTGCAGGCC
    CCCTTCTACA CTCGTAACCA CCCCAAGGCC CTGAACTTTG GTGGAATCGG CGTGGTGATG
    GGCCATGAGT TGACACATGC GTTTGATGAC CAAGGTCGCG AATATGACAA GGAAGGGAAC
    CTGCGGCCCT GGTGGCAGAA TGAGTCCCTG GCAGCCTTCC GGAATCACAC AGCTTGCATG
    GAGGAACAGT ACAGCCAGTA CCAGGTCAAC GGGGAGAGGC TCAATGGGCG CCAGACGCTG
    GGGGAGAACA TCGCTGACAA CGGGGGGCTG AAGGCTGCCT ATAACGCTTA CAAAGCATGG
    CTGAGCAAGC ATGGGGAAGA GCAGCAGCTG CCAGCCGTGG GGCTCACCAA TCACCAGCTC
    TTCTTTGTGG GATTTGCCCA GGTGTGGTGC TCAGTCCGCA CACCCGAGAG CTCTCACGAG
    GGGCTGGTGA CTGACCCCCA CAGCCCCGCC CGCTTCCGCG TGCTGGGCAC TCTCTCCAAC
    TCCCGGGACT TCCTGCGACA CTTCGGCTGC CCTGTCGGCT CCCCCATGAA CCCAGGGCAG
    CTGTGTGAGG TGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_859328.1
    CDS83..2380
    Translation

    Target ORF information:

    RefSeq Version XM_854235.3
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris endothelin converting enzyme 2 (ECE2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_854235.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAACGTCG CGCTGCAGGA GCTGGGCGGC GGCGGCAACA TGGTGGAGTA CGAGCGGACC 
    ACGCTGCGGG AGGAAGACGC ACCTGAGACC CCCGTAGAGG GCGGGGCCTC CCCGGACGCT
    GTGGAGGTGG GATTCCGGAA GAGGACAAGA GACTTCTTGG GCTCGCACAC CCAACTGGAG
    CTGGTCTTAG CCGCTGTCTC TCTACTGCTG GCTGCACTGC TACTGGGCTG CTTGGTGGCT
    CTGGGGGTCC AGTACCACGG GGACCCATCC CACAACACCT GCCTCACAGA GGCCTGCATT
    CGAGTAGCTG GAAAGATTCT GGAGTCCCTG GACCGCGGGG TGAGTCCCTG TGAGGACTTT
    TACCAGTTTT CCTGTGGTGG CTGGATTCAG AGAAATCCTC TGCCCGATGG GCGTTCTCGT
    TGGAACACCT TCAACAGCCT CTGGGACCAA AATCAGGCCA TACTGAAGCA CCTGCTTGAA
    AACACCACCT TCAACTCTAC CAGTGAAGCT GAGCGGAAGA CACAGCGTTT CTACCTCTCC
    TGCCTGCAGG TGGAGCGGAT CGAGGAACTG GGAGCCCAGC CACTGCGAGA CCTCATTGAC
    AAGATTGGTG GTTGGAACAT TACCGGACCC TGGGACCAGG ACAACTTCAT GGAAGTGCTG
    AAGGCAGTAG CAGGGACCTA CAGAGCTACT CCCTTCTTCA CTGTCTACGT CAGTGCCGAC
    TCTAAGAGCT CTAACAGCAA TGTTATCCAG GTGGACCAAT CTGGGCTCTT TCTCCCCTCT
    CGAGACTACT ACCTAAACAG GACCGCCAAT GAAAAAGTGC TTACCGCCTA CCTTGACTAC
    ATGGAGGAGC TGGGGATGTT GCTGGGTGGA CGGCCAACCT CCACACGGGA GCAGATGCGG
    CAGGTGCTGG AGCTGGAGAT ACAACTGGCC AACATCACAG TGCCCCAGGA CCAGAGGCGC
    GATGAGGAGA AGATCTATCA TAAGATGAAC ATTGCAGAGC TGCAGGCCCT GGCACCCTCT
    GTGGACTGGC TGGAGTTTCT GTCTTTCTTG TTGTCACCGC TGGAGCTGGG TGACTCTGAA
    CCTGTGGTGG TGTATGGAAC GGATTATTTG CAGCAGGTGT CAGAGCTCAT CAACCACACA
    GAGCCAAGTG TCCTGAACAA TTACCTGATT TGGAATCTGG TACAGAAGAC AACCTCAAGC
    CTGGACCACC GCTTTGAATC TGCACAGGAG AAACTGCTGG AGACTCTCTA TGGCACCAAG
    AAGTCCTGCA TGCCAAGGTG GCAGACCTGC ATCTCCAACA CGGACGACGC ACTTGGCTTC
    GCTTTGGGCT CCCTCTTTGT GAAGGCCACG TTCGATCGGC AAAGCAAGGA AATTGCTGAA
    GGGATGATCA GCGAGATCCG GAATGCCTTT GAGGAAGCCC TGGGGCAGCT GGTTTGGATG
    GATGAGAAGA CCCGCCAGGC AGCCAAGGAG AAAGCAGATG CTATATATGA TATGATTGGT
    TTCCCGGACT TCATCCTGGA TCCCAAAGAG CTGGATGATG TTTATGATGG GTACGAAGTC
    TCTGAAGATT CCTTCTTCCA GAACATGTTG AATTTGTACA ATTTCTCTGC TAAGGTGATG
    GCTGACCAGC TCCGCAAGCC TCCCAGCCGG GACCAGTGGA GCATGACCCC CCAGACAGTG
    AATGCCTACT ACCTTCCAAC CAAGAATGAA ATTGTCTTCC CTGCCGGCAT CTTGCAGGCC
    CCCTTCTACA CTCGTAACCA CCCCAAGGCC CTGAACTTTG GTGGAATCGG CGTGGTGATG
    GGCCATGAGT TGACACATGC GTTTGATGAC CAAGGTCGCG AATATGACAA GGAAGGGAAC
    CTGCGGCCCT GGTGGCAGAA TGAGTCCCTG GCAGCCTTCC GGAATCACAC AGCTTGCATG
    GAGGAACAGT ACAGCCAGTA CCAGGTCAAC GGGGAGAGGC TCAATGGGCG CCAGACGCTG
    GGGGAGAACA TCGCTGACAA CGGGGGGCTG AAGGCTGCCT ATAACGCTTA CAAAGCATGG
    CTGAGCAAGC ATGGGGAAGA GCAGCAGCTG CCAGCCGTGG GGCTCACCAA TCACCAGCTC
    TTCTTTGTGG GATTTGCCCA GGTGTGGTGC TCAGTCCGCA CACCCGAGAG CTCTCACGAG
    GGGCTGGTGA CTGACCCCCA CAGCCCCGCC CGCTTCCGCG TGCTGGGCAC TCTCTCCAAC
    TCCCGGGACT TCCTGCGACA CTTCGGCTGC CCTGTCGGCT CCCCCATGAA CCCAGGGCAG
    CTGTGTGAGG TGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30455
    Clone ID Related Accession (Same CDS sequence) XM_003434114.2 , XM_003434114.3
    Accession Version XM_003434114.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2211bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product endothelin-converting enzyme 2 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726120.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2013 this sequence version replaced XM_003434114.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAACGTCG CGCTGCAGGA GCTGGGCGGC GGCGGCAACG TGGGATTCCG GAAGAGGACA 
    AGAGACTTCT TGGGCTCGCA CACCCAACTG GAGCTGGTCT TAGCCGCTGT CTCTCTACTG
    CTGGCTGCAC TGCTACTGGG CTGCTTGGTG GCTCTGGGGG TCCAGTACCA CGGGGACCCA
    TCCCACAACA CCTGCCTCAC AGAGGCCTGC ATTCGAGTAG CTGGAAAGAT TCTGGAGTCC
    CTGGACCGCG GGGTGAGTCC CTGTGAGGAC TTTTACCAGT TTTCCTGTGG TGGCTGGATT
    CAGAGAAATC CTCTGCCCGA TGGGCGTTCT CGTTGGAACA CCTTCAACAG CCTCTGGGAC
    CAAAATCAGG CCATACTGAA GCACCTGCTT GAAAACACCA CCTTCAACTC TACCAGTGAA
    GCTGAGCGGA AGACACAGCG TTTCTACCTC TCCTGCCTGC AGGTGGAGCG GATCGAGGAA
    CTGGGAGCCC AGCCACTGCG AGACCTCATT GACAAGATTG GTGGTTGGAA CATTACCGGA
    CCCTGGGACC AGGACAACTT CATGGAAGTG CTGAAGGCAG TAGCAGGGAC CTACAGAGCT
    ACTCCCTTCT TCACTGTCTA CGTCAGTGCC GACTCTAAGA GCTCTAACAG CAATGTTATC
    CAGGTGGACC AATCTGGGCT CTTTCTCCCC TCTCGAGACT ACTACCTAAA CAGGACCGCC
    AATGAAAAAG TGCTTACCGC CTACCTTGAC TACATGGAGG AGCTGGGGAT GTTGCTGGGT
    GGACGGCCAA CCTCCACACG GGAGCAGATG CGGCAGGTGC TGGAGCTGGA GATACAACTG
    GCCAACATCA CAGTGCCCCA GGACCAGAGG CGCGATGAGG AGAAGATCTA TCATAAGATG
    AACATTGCAG AGCTGCAGGC CCTGGCACCC TCTGTGGACT GGCTGGAGTT TCTGTCTTTC
    TTGTTGTCAC CGCTGGAGCT GGGTGACTCT GAACCTGTGG TGGTGTATGG AACGGATTAT
    TTGCAGCAGG TGTCAGAGCT CATCAACCAC ACAGAGCCAA GTGTCCTGAA CAATTACCTG
    ATTTGGAATC TGGTACAGAA GACAACCTCA AGCCTGGACC ACCGCTTTGA ATCTGCACAG
    GAGAAACTGC TGGAGACTCT CTATGGCACC AAGAAGTCCT GCATGCCAAG GTGGCAGACC
    TGCATCTCCA ACACGGACGA CGCACTTGGC TTCGCTTTGG GCTCCCTCTT TGTGAAGGCC
    ACGTTCGATC GGCAAAGCAA GGAAATTGCT GAAGGGATGA TCAGCGAGAT CCGGAATGCC
    TTTGAGGAAG CCCTGGGGCA GCTGGTTTGG ATGGATGAGA AGACCCGCCA GGCAGCCAAG
    GAGAAAGCAG ATGCTATATA TGATATGATT GGTTTCCCGG ACTTCATCCT GGATCCCAAA
    GAGCTGGATG ATGTTTATGA TGGGTACGAA GTCTCTGAAG ATTCCTTCTT CCAGAACATG
    TTGAATTTGT ACAATTTCTC TGCTAAGGTG ATGGCTGACC AGCTCCGCAA GCCTCCCAGC
    CGGGACCAGT GGAGCATGAC CCCCCAGACA GTGAATGCCT ACTACCTTCC AACCAAGAAT
    GAAATTGTCT TCCCTGCCGG CATCTTGCAG GCCCCCTTCT ACACTCGTAA CCACCCCAAG
    GCCCTGAACT TTGGTGGAAT CGGCGTGGTG ATGGGCCATG AGTTGACACA TGCGTTTGAT
    GACCAAGGTC GCGAATATGA CAAGGAAGGG AACCTGCGGC CCTGGTGGCA GAATGAGTCC
    CTGGCAGCCT TCCGGAATCA CACAGCTTGC ATGGAGGAAC AGTACAGCCA GTACCAGGTC
    AACGGGGAGA GGCTCAATGG GCGCCAGACG CTGGGGGAGA ACATCGCTGA CAACGGGGGG
    CTGAAGGCTG CCTATAACGC TTACAAAGCA TGGCTGAGCA AGCATGGGGA AGAGCAGCAG
    CTGCCAGCCG TGGGGCTCAC CAATCACCAG CTCTTCTTTG TGGGATTTGC CCAGGTGTGG
    TGCTCAGTCC GCACACCCGA GAGCTCTCAC GAGGGGCTGG TGACTGACCC CCACAGCCCC
    GCCCGCTTCC GCGTGCTGGG CACTCTCTCC AACTCCCGGG ACTTCCTGCG ACACTTCGGC
    TGCCCTGTCG GCTCCCCCAT GAACCCAGGG CAGCTGTGTG AGGTGTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003434162.1
    CDS83..2293
    Translation

    Target ORF information:

    RefSeq Version XM_003434114.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris endothelin converting enzyme 2 (ECE2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003434114.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAACGTCG CGCTGCAGGA GCTGGGCGGC GGCGGCAACG TGGGATTCCG GAAGAGGACA 
    AGAGACTTCT TGGGCTCGCA CACCCAACTG GAGCTGGTCT TAGCCGCTGT CTCTCTACTG
    CTGGCTGCAC TGCTACTGGG CTGCTTGGTG GCTCTGGGGG TCCAGTACCA CGGGGACCCA
    TCCCACAACA CCTGCCTCAC AGAGGCCTGC ATTCGAGTAG CTGGAAAGAT TCTGGAGTCC
    CTGGACCGCG GGGTGAGTCC CTGTGAGGAC TTTTACCAGT TTTCCTGTGG TGGCTGGATT
    CAGAGAAATC CTCTGCCCGA TGGGCGTTCT CGTTGGAACA CCTTCAACAG CCTCTGGGAC
    CAAAATCAGG CCATACTGAA GCACCTGCTT GAAAACACCA CCTTCAACTC TACCAGTGAA
    GCTGAGCGGA AGACACAGCG TTTCTACCTC TCCTGCCTGC AGGTGGAGCG GATCGAGGAA
    CTGGGAGCCC AGCCACTGCG AGACCTCATT GACAAGATTG GTGGTTGGAA CATTACCGGA
    CCCTGGGACC AGGACAACTT CATGGAAGTG CTGAAGGCAG TAGCAGGGAC CTACAGAGCT
    ACTCCCTTCT TCACTGTCTA CGTCAGTGCC GACTCTAAGA GCTCTAACAG CAATGTTATC
    CAGGTGGACC AATCTGGGCT CTTTCTCCCC TCTCGAGACT ACTACCTAAA CAGGACCGCC
    AATGAAAAAG TGCTTACCGC CTACCTTGAC TACATGGAGG AGCTGGGGAT GTTGCTGGGT
    GGACGGCCAA CCTCCACACG GGAGCAGATG CGGCAGGTGC TGGAGCTGGA GATACAACTG
    GCCAACATCA CAGTGCCCCA GGACCAGAGG CGCGATGAGG AGAAGATCTA TCATAAGATG
    AACATTGCAG AGCTGCAGGC CCTGGCACCC TCTGTGGACT GGCTGGAGTT TCTGTCTTTC
    TTGTTGTCAC CGCTGGAGCT GGGTGACTCT GAACCTGTGG TGGTGTATGG AACGGATTAT
    TTGCAGCAGG TGTCAGAGCT CATCAACCAC ACAGAGCCAA GTGTCCTGAA CAATTACCTG
    ATTTGGAATC TGGTACAGAA GACAACCTCA AGCCTGGACC ACCGCTTTGA ATCTGCACAG
    GAGAAACTGC TGGAGACTCT CTATGGCACC AAGAAGTCCT GCATGCCAAG GTGGCAGACC
    TGCATCTCCA ACACGGACGA CGCACTTGGC TTCGCTTTGG GCTCCCTCTT TGTGAAGGCC
    ACGTTCGATC GGCAAAGCAA GGAAATTGCT GAAGGGATGA TCAGCGAGAT CCGGAATGCC
    TTTGAGGAAG CCCTGGGGCA GCTGGTTTGG ATGGATGAGA AGACCCGCCA GGCAGCCAAG
    GAGAAAGCAG ATGCTATATA TGATATGATT GGTTTCCCGG ACTTCATCCT GGATCCCAAA
    GAGCTGGATG ATGTTTATGA TGGGTACGAA GTCTCTGAAG ATTCCTTCTT CCAGAACATG
    TTGAATTTGT ACAATTTCTC TGCTAAGGTG ATGGCTGACC AGCTCCGCAA GCCTCCCAGC
    CGGGACCAGT GGAGCATGAC CCCCCAGACA GTGAATGCCT ACTACCTTCC AACCAAGAAT
    GAAATTGTCT TCCCTGCCGG CATCTTGCAG GCCCCCTTCT ACACTCGTAA CCACCCCAAG
    GCCCTGAACT TTGGTGGAAT CGGCGTGGTG ATGGGCCATG AGTTGACACA TGCGTTTGAT
    GACCAAGGTC GCGAATATGA CAAGGAAGGG AACCTGCGGC CCTGGTGGCA GAATGAGTCC
    CTGGCAGCCT TCCGGAATCA CACAGCTTGC ATGGAGGAAC AGTACAGCCA GTACCAGGTC
    AACGGGGAGA GGCTCAATGG GCGCCAGACG CTGGGGGAGA ACATCGCTGA CAACGGGGGG
    CTGAAGGCTG CCTATAACGC TTACAAAGCA TGGCTGAGCA AGCATGGGGA AGAGCAGCAG
    CTGCCAGCCG TGGGGCTCAC CAATCACCAG CTCTTCTTTG TGGGATTTGC CCAGGTGTGG
    TGCTCAGTCC GCACACCCGA GAGCTCTCAC GAGGGGCTGG TGACTGACCC CCACAGCCCC
    GCCCGCTTCC GCGTGCTGGG CACTCTCTCC AACTCCCGGG ACTTCCTGCG ACACTTCGGC
    TGCCCTGTCG GCTCCCCCAT GAACCCAGGG CAGCTGTGTG AGGTGTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30455
    Clone ID Related Accession (Same CDS sequence) XM_003434114.2 , XM_003434114.3
    Accession Version XM_003434114.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2211bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product endothelin-converting enzyme 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003434114.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAACGTCG CGCTGCAGGA GCTGGGCGGC GGCGGCAACG TGGGATTCCG GAAGAGGACA 
    AGAGACTTCT TGGGCTCGCA CACCCAACTG GAGCTGGTCT TAGCCGCTGT CTCTCTACTG
    CTGGCTGCAC TGCTACTGGG CTGCTTGGTG GCTCTGGGGG TCCAGTACCA CGGGGACCCA
    TCCCACAACA CCTGCCTCAC AGAGGCCTGC ATTCGAGTAG CTGGAAAGAT TCTGGAGTCC
    CTGGACCGCG GGGTGAGTCC CTGTGAGGAC TTTTACCAGT TTTCCTGTGG TGGCTGGATT
    CAGAGAAATC CTCTGCCCGA TGGGCGTTCT CGTTGGAACA CCTTCAACAG CCTCTGGGAC
    CAAAATCAGG CCATACTGAA GCACCTGCTT GAAAACACCA CCTTCAACTC TACCAGTGAA
    GCTGAGCGGA AGACACAGCG TTTCTACCTC TCCTGCCTGC AGGTGGAGCG GATCGAGGAA
    CTGGGAGCCC AGCCACTGCG AGACCTCATT GACAAGATTG GTGGTTGGAA CATTACCGGA
    CCCTGGGACC AGGACAACTT CATGGAAGTG CTGAAGGCAG TAGCAGGGAC CTACAGAGCT
    ACTCCCTTCT TCACTGTCTA CGTCAGTGCC GACTCTAAGA GCTCTAACAG CAATGTTATC
    CAGGTGGACC AATCTGGGCT CTTTCTCCCC TCTCGAGACT ACTACCTAAA CAGGACCGCC
    AATGAAAAAG TGCTTACCGC CTACCTTGAC TACATGGAGG AGCTGGGGAT GTTGCTGGGT
    GGACGGCCAA CCTCCACACG GGAGCAGATG CGGCAGGTGC TGGAGCTGGA GATACAACTG
    GCCAACATCA CAGTGCCCCA GGACCAGAGG CGCGATGAGG AGAAGATCTA TCATAAGATG
    AACATTGCAG AGCTGCAGGC CCTGGCACCC TCTGTGGACT GGCTGGAGTT TCTGTCTTTC
    TTGTTGTCAC CGCTGGAGCT GGGTGACTCT GAACCTGTGG TGGTGTATGG AACGGATTAT
    TTGCAGCAGG TGTCAGAGCT CATCAACCAC ACAGAGCCAA GTGTCCTGAA CAATTACCTG
    ATTTGGAATC TGGTACAGAA GACAACCTCA AGCCTGGACC ACCGCTTTGA ATCTGCACAG
    GAGAAACTGC TGGAGACTCT CTATGGCACC AAGAAGTCCT GCATGCCAAG GTGGCAGACC
    TGCATCTCCA ACACGGACGA CGCACTTGGC TTCGCTTTGG GCTCCCTCTT TGTGAAGGCC
    ACGTTCGATC GGCAAAGCAA GGAAATTGCT GAAGGGATGA TCAGCGAGAT CCGGAATGCC
    TTTGAGGAAG CCCTGGGGCA GCTGGTTTGG ATGGATGAGA AGACCCGCCA GGCAGCCAAG
    GAGAAAGCAG ATGCTATATA TGATATGATT GGTTTCCCGG ACTTCATCCT GGATCCCAAA
    GAGCTGGATG ATGTTTATGA TGGGTACGAA GTCTCTGAAG ATTCCTTCTT CCAGAACATG
    TTGAATTTGT ACAATTTCTC TGCTAAGGTG ATGGCTGACC AGCTCCGCAA GCCTCCCAGC
    CGGGACCAGT GGAGCATGAC CCCCCAGACA GTGAATGCCT ACTACCTTCC AACCAAGAAT
    GAAATTGTCT TCCCTGCCGG CATCTTGCAG GCCCCCTTCT ACACTCGTAA CCACCCCAAG
    GCCCTGAACT TTGGTGGAAT CGGCGTGGTG ATGGGCCATG AGTTGACACA TGCGTTTGAT
    GACCAAGGTC GCGAATATGA CAAGGAAGGG AACCTGCGGC CCTGGTGGCA GAATGAGTCC
    CTGGCAGCCT TCCGGAATCA CACAGCTTGC ATGGAGGAAC AGTACAGCCA GTACCAGGTC
    AACGGGGAGA GGCTCAATGG GCGCCAGACG CTGGGGGAGA ACATCGCTGA CAACGGGGGG
    CTGAAGGCTG CCTATAACGC TTACAAAGCA TGGCTGAGCA AGCATGGGGA AGAGCAGCAG
    CTGCCAGCCG TGGGGCTCAC CAATCACCAG CTCTTCTTTG TGGGATTTGC CCAGGTGTGG
    TGCTCAGTCC GCACACCCGA GAGCTCTCAC GAGGGGCTGG TGACTGACCC CCACAGCCCC
    GCCCGCTTCC GCGTGCTGGG CACTCTCTCC AACTCCCGGG ACTTCCTGCG ACACTTCGGC
    TGCCCTGTCG GCTCCCCCAT GAACCCAGGG CAGCTGTGTG AGGTGTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003434162.1
    CDS83..2293
    Translation

    Target ORF information:

    RefSeq Version XM_003434114.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris endothelin converting enzyme 2 (ECE2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003434114.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAACGTCG CGCTGCAGGA GCTGGGCGGC GGCGGCAACG TGGGATTCCG GAAGAGGACA 
    AGAGACTTCT TGGGCTCGCA CACCCAACTG GAGCTGGTCT TAGCCGCTGT CTCTCTACTG
    CTGGCTGCAC TGCTACTGGG CTGCTTGGTG GCTCTGGGGG TCCAGTACCA CGGGGACCCA
    TCCCACAACA CCTGCCTCAC AGAGGCCTGC ATTCGAGTAG CTGGAAAGAT TCTGGAGTCC
    CTGGACCGCG GGGTGAGTCC CTGTGAGGAC TTTTACCAGT TTTCCTGTGG TGGCTGGATT
    CAGAGAAATC CTCTGCCCGA TGGGCGTTCT CGTTGGAACA CCTTCAACAG CCTCTGGGAC
    CAAAATCAGG CCATACTGAA GCACCTGCTT GAAAACACCA CCTTCAACTC TACCAGTGAA
    GCTGAGCGGA AGACACAGCG TTTCTACCTC TCCTGCCTGC AGGTGGAGCG GATCGAGGAA
    CTGGGAGCCC AGCCACTGCG AGACCTCATT GACAAGATTG GTGGTTGGAA CATTACCGGA
    CCCTGGGACC AGGACAACTT CATGGAAGTG CTGAAGGCAG TAGCAGGGAC CTACAGAGCT
    ACTCCCTTCT TCACTGTCTA CGTCAGTGCC GACTCTAAGA GCTCTAACAG CAATGTTATC
    CAGGTGGACC AATCTGGGCT CTTTCTCCCC TCTCGAGACT ACTACCTAAA CAGGACCGCC
    AATGAAAAAG TGCTTACCGC CTACCTTGAC TACATGGAGG AGCTGGGGAT GTTGCTGGGT
    GGACGGCCAA CCTCCACACG GGAGCAGATG CGGCAGGTGC TGGAGCTGGA GATACAACTG
    GCCAACATCA CAGTGCCCCA GGACCAGAGG CGCGATGAGG AGAAGATCTA TCATAAGATG
    AACATTGCAG AGCTGCAGGC CCTGGCACCC TCTGTGGACT GGCTGGAGTT TCTGTCTTTC
    TTGTTGTCAC CGCTGGAGCT GGGTGACTCT GAACCTGTGG TGGTGTATGG AACGGATTAT
    TTGCAGCAGG TGTCAGAGCT CATCAACCAC ACAGAGCCAA GTGTCCTGAA CAATTACCTG
    ATTTGGAATC TGGTACAGAA GACAACCTCA AGCCTGGACC ACCGCTTTGA ATCTGCACAG
    GAGAAACTGC TGGAGACTCT CTATGGCACC AAGAAGTCCT GCATGCCAAG GTGGCAGACC
    TGCATCTCCA ACACGGACGA CGCACTTGGC TTCGCTTTGG GCTCCCTCTT TGTGAAGGCC
    ACGTTCGATC GGCAAAGCAA GGAAATTGCT GAAGGGATGA TCAGCGAGAT CCGGAATGCC
    TTTGAGGAAG CCCTGGGGCA GCTGGTTTGG ATGGATGAGA AGACCCGCCA GGCAGCCAAG
    GAGAAAGCAG ATGCTATATA TGATATGATT GGTTTCCCGG ACTTCATCCT GGATCCCAAA
    GAGCTGGATG ATGTTTATGA TGGGTACGAA GTCTCTGAAG ATTCCTTCTT CCAGAACATG
    TTGAATTTGT ACAATTTCTC TGCTAAGGTG ATGGCTGACC AGCTCCGCAA GCCTCCCAGC
    CGGGACCAGT GGAGCATGAC CCCCCAGACA GTGAATGCCT ACTACCTTCC AACCAAGAAT
    GAAATTGTCT TCCCTGCCGG CATCTTGCAG GCCCCCTTCT ACACTCGTAA CCACCCCAAG
    GCCCTGAACT TTGGTGGAAT CGGCGTGGTG ATGGGCCATG AGTTGACACA TGCGTTTGAT
    GACCAAGGTC GCGAATATGA CAAGGAAGGG AACCTGCGGC CCTGGTGGCA GAATGAGTCC
    CTGGCAGCCT TCCGGAATCA CACAGCTTGC ATGGAGGAAC AGTACAGCCA GTACCAGGTC
    AACGGGGAGA GGCTCAATGG GCGCCAGACG CTGGGGGAGA ACATCGCTGA CAACGGGGGG
    CTGAAGGCTG CCTATAACGC TTACAAAGCA TGGCTGAGCA AGCATGGGGA AGAGCAGCAG
    CTGCCAGCCG TGGGGCTCAC CAATCACCAG CTCTTCTTTG TGGGATTTGC CCAGGTGTGG
    TGCTCAGTCC GCACACCCGA GAGCTCTCAC GAGGGGCTGG TGACTGACCC CCACAGCCCC
    GCCCGCTTCC GCGTGCTGGG CACTCTCTCC AACTCCCGGG ACTTCCTGCG ACACTTCGGC
    TGCCCTGTCG GCTCCCCCAT GAACCCAGGG CAGCTGTGTG AGGTGTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44828
    Clone ID Related Accession (Same CDS sequence) XM_014110394.2 , XM_014110394.1
    Accession Version XM_014110394.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2388bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product endothelin-converting enzyme 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014110394.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGAACGTCG CGCTGCAGGA GCTGGGCGGC GGCGGCAACA TGGTGGAGTA CGAGCGGACC 
    ACGCTGCGGG AGGAAGACGC ACCTGAGACC CCCGTAGAGG GCGGGGCCTC CCCGGACGCT
    GTGGAGGTGG GATTCCGGAA GAGGACAAGA GACTTCTTGG GCTCGCACAC CCAACTGGAG
    CTGGTCTTAG CCGCTGTCTC TCTACTGCTG GCTGCACTGC TACTGGGCTG CTTGGTGGCT
    CTGGGGGTCC AGTACCACGG GGACCCATCC CACAACACCT GCCTCACAGA GGCCTGCATT
    CGAGTAGCTG GAAAGATTCT GGAGTCCCTG GACCGCGGGG TGAGTCCCTG TGAGGACTTT
    TACCAGTTTT CCTGTGGTGG CTGGATTCAG AGAAATCCTC TGCCCGATGG GCGTTCTCGT
    TGGAACACCT TCAACAGCCT CTGGGACCAA AATCAGGCCA TACTGAAGCA CCTGCTTGAA
    AACACCACCT TCAACTCTAC CAGTGAAGCT GAGCGGAAGA CACAGCGTTT CTACCTCTCC
    TGCCTGCAGG TGGAGCGGAT CGAGGAACTG GGAGCCCAGC CACTGCGAGA CCTCATTGAC
    AAGATTGGTG GTTGGAACAT TACCGGACCC TGGGACCAGG ACAACTTCAT GGAAGTGCTG
    AAGGCAGTAG CAGGGACCTA CAGAGCTACT CCCTTCTTCA CTGTCTACGT CAGTGCCGAC
    TCTAAGAGCT CTAACAGCAA TGTTATCCAG GTGGACCAAT CTGGGCTCTT TCTCCCCTCT
    CGAGACTACT ACCTAAACAG GACCGCCAAT GAAAAAGTGC TTACCGCCTA CCTTGACTAC
    ATGGAGGAGC TGGGGATGTT GCTGGGTGGA CGGCCAACCT CCACACGGGA GCAGATGCGG
    CAGGTGCTGG AGCTGGAGAT ACAACTGGCC AACATCACAG TGCCCCAGGA CCAGAGGCGC
    GATGAGGAGA AGATCTATCA TAAGATGAAC ATTGCAGAGC TGCAGGCCCT GGCACCCTCT
    GTGGACTGGC TGGAGTTTCT GTCTTTCTTG TTGTCACCGC TGGAGCTGGG TGACTCTGAA
    CCTGTGGTGG TGTATGGAAC GGATTATTTG CAGCAGGTGT CAGAGCTCAT CAACCACACA
    GAGCCAAGTG TCCTGAACAA TTACCTGATT TGGAATCTGG TACAGAAGAC AACCTCAAGC
    CTGGACCACC GCTTTGAATC TGCACAGGAG AAACTGCTGG AGACTCTCTA TGGCACCAAG
    AAGAGCCGAG AGGGCTGGCC CCAGAGAAGA GAAAGGCCCT CAGACTGGAG AACAGAGCTA
    TCTCTGGGAG GCCTGGCTCT TTGTCCCTTA CAGTCCTGCA TGCCAAGGTG GCAGACCTGC
    ATCTCCAACA CGGACGACGC ACTTGGCTTC GCTTTGGGCT CCCTCTTTGT GAAGGCCACG
    TTCGATCGGC AAAGCAAGGA AATTGCTGAA GGGATGATCA GCGAGATCCG GAATGCCTTT
    GAGGAAGCCC TGGGGCAGCT GGTTTGGATG GATGAGAAGA CCCGCCAGGC AGCCAAGGAG
    AAAGCAGATG CTATATATGA TATGATTGGT TTCCCGGACT TCATCCTGGA TCCCAAAGAG
    CTGGATGATG TTTATGATGG GTACGAAGTC TCTGAAGATT CCTTCTTCCA GAACATGTTG
    AATTTGTACA ATTTCTCTGC TAAGGTGATG GCTGACCAGC TCCGCAAGCC TCCCAGCCGG
    GACCAGTGGA GCATGACCCC CCAGACAGTG AATGCCTACT ACCTTCCAAC CAAGAATGAA
    ATTGTCTTCC CTGCCGGCAT CTTGCAGGCC CCCTTCTACA CTCGTAACCA CCCCAAGGCC
    CTGAACTTTG GTGGAATCGG CGTGGTGATG GGCCATGAGT TGACACATGC GTTTGATGAC
    CAAGGTCGCG AATATGACAA GGAAGGGAAC CTGCGGCCCT GGTGGCAGAA TGAGTCCCTG
    GCAGCCTTCC GGAATCACAC AGCTTGCATG GAGGAACAGT ACAGCCAGTA CCAGGTCAAC
    GGGGAGAGGC TCAATGGGCG CCAGACGCTG GGGGAGAACA TCGCTGACAA CGGGGGGCTG
    AAGGCTGCCT ATAACGCTTA CAAAGCATGG CTGAGCAAGC ATGGGGAAGA GCAGCAGCTG
    CCAGCCGTGG GGCTCACCAA TCACCAGCTC TTCTTTGTGG GATTTGCCCA GGTGTGGTGC
    TCAGTCCGCA CACCCGAGAG CTCTCACGAG GGGCTGGTGA CTGACCCCCA CAGCCCCGCC
    CGCTTCCGCG TGCTGGGCAC TCTCTCCAAC TCCCGGGACT TCCTGCGACA CTTCGGCTGC
    CCTGTCGGCT CCCCCATGAA CCCAGGGCAG CTGTGTGAGG TGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965869.1
    CDS90..2477
    Translation

    Target ORF information:

    RefSeq Version XM_014110394.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris endothelin converting enzyme 2 (ECE2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014110394.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGAACGTCG CGCTGCAGGA GCTGGGCGGC GGCGGCAACA TGGTGGAGTA CGAGCGGACC 
    ACGCTGCGGG AGGAAGACGC ACCTGAGACC CCCGTAGAGG GCGGGGCCTC CCCGGACGCT
    GTGGAGGTGG GATTCCGGAA GAGGACAAGA GACTTCTTGG GCTCGCACAC CCAACTGGAG
    CTGGTCTTAG CCGCTGTCTC TCTACTGCTG GCTGCACTGC TACTGGGCTG CTTGGTGGCT
    CTGGGGGTCC AGTACCACGG GGACCCATCC CACAACACCT GCCTCACAGA GGCCTGCATT
    CGAGTAGCTG GAAAGATTCT GGAGTCCCTG GACCGCGGGG TGAGTCCCTG TGAGGACTTT
    TACCAGTTTT CCTGTGGTGG CTGGATTCAG AGAAATCCTC TGCCCGATGG GCGTTCTCGT
    TGGAACACCT TCAACAGCCT CTGGGACCAA AATCAGGCCA TACTGAAGCA CCTGCTTGAA
    AACACCACCT TCAACTCTAC CAGTGAAGCT GAGCGGAAGA CACAGCGTTT CTACCTCTCC
    TGCCTGCAGG TGGAGCGGAT CGAGGAACTG GGAGCCCAGC CACTGCGAGA CCTCATTGAC
    AAGATTGGTG GTTGGAACAT TACCGGACCC TGGGACCAGG ACAACTTCAT GGAAGTGCTG
    AAGGCAGTAG CAGGGACCTA CAGAGCTACT CCCTTCTTCA CTGTCTACGT CAGTGCCGAC
    TCTAAGAGCT CTAACAGCAA TGTTATCCAG GTGGACCAAT CTGGGCTCTT TCTCCCCTCT
    CGAGACTACT ACCTAAACAG GACCGCCAAT GAAAAAGTGC TTACCGCCTA CCTTGACTAC
    ATGGAGGAGC TGGGGATGTT GCTGGGTGGA CGGCCAACCT CCACACGGGA GCAGATGCGG
    CAGGTGCTGG AGCTGGAGAT ACAACTGGCC AACATCACAG TGCCCCAGGA CCAGAGGCGC
    GATGAGGAGA AGATCTATCA TAAGATGAAC ATTGCAGAGC TGCAGGCCCT GGCACCCTCT
    GTGGACTGGC TGGAGTTTCT GTCTTTCTTG TTGTCACCGC TGGAGCTGGG TGACTCTGAA
    CCTGTGGTGG TGTATGGAAC GGATTATTTG CAGCAGGTGT CAGAGCTCAT CAACCACACA
    GAGCCAAGTG TCCTGAACAA TTACCTGATT TGGAATCTGG TACAGAAGAC AACCTCAAGC
    CTGGACCACC GCTTTGAATC TGCACAGGAG AAACTGCTGG AGACTCTCTA TGGCACCAAG
    AAGAGCCGAG AGGGCTGGCC CCAGAGAAGA GAAAGGCCCT CAGACTGGAG AACAGAGCTA
    TCTCTGGGAG GCCTGGCTCT TTGTCCCTTA CAGTCCTGCA TGCCAAGGTG GCAGACCTGC
    ATCTCCAACA CGGACGACGC ACTTGGCTTC GCTTTGGGCT CCCTCTTTGT GAAGGCCACG
    TTCGATCGGC AAAGCAAGGA AATTGCTGAA GGGATGATCA GCGAGATCCG GAATGCCTTT
    GAGGAAGCCC TGGGGCAGCT GGTTTGGATG GATGAGAAGA CCCGCCAGGC AGCCAAGGAG
    AAAGCAGATG CTATATATGA TATGATTGGT TTCCCGGACT TCATCCTGGA TCCCAAAGAG
    CTGGATGATG TTTATGATGG GTACGAAGTC TCTGAAGATT CCTTCTTCCA GAACATGTTG
    AATTTGTACA ATTTCTCTGC TAAGGTGATG GCTGACCAGC TCCGCAAGCC TCCCAGCCGG
    GACCAGTGGA GCATGACCCC CCAGACAGTG AATGCCTACT ACCTTCCAAC CAAGAATGAA
    ATTGTCTTCC CTGCCGGCAT CTTGCAGGCC CCCTTCTACA CTCGTAACCA CCCCAAGGCC
    CTGAACTTTG GTGGAATCGG CGTGGTGATG GGCCATGAGT TGACACATGC GTTTGATGAC
    CAAGGTCGCG AATATGACAA GGAAGGGAAC CTGCGGCCCT GGTGGCAGAA TGAGTCCCTG
    GCAGCCTTCC GGAATCACAC AGCTTGCATG GAGGAACAGT ACAGCCAGTA CCAGGTCAAC
    GGGGAGAGGC TCAATGGGCG CCAGACGCTG GGGGAGAACA TCGCTGACAA CGGGGGGCTG
    AAGGCTGCCT ATAACGCTTA CAAAGCATGG CTGAGCAAGC ATGGGGAAGA GCAGCAGCTG
    CCAGCCGTGG GGCTCACCAA TCACCAGCTC TTCTTTGTGG GATTTGCCCA GGTGTGGTGC
    TCAGTCCGCA CACCCGAGAG CTCTCACGAG GGGCTGGTGA CTGACCCCCA CAGCCCCGCC
    CGCTTCCGCG TGCTGGGCAC TCTCTCCAAC TCCCGGGACT TCCTGCGACA CTTCGGCTGC
    CCTGTCGGCT CCCCCATGAA CCCAGGGCAG CTGTGTGAGG TGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44828
    Clone ID Related Accession (Same CDS sequence) XM_014110394.2 , XM_014110394.1
    Accession Version XM_014110394.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2388bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product endothelin-converting enzyme 2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726120.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGAACGTCG CGCTGCAGGA GCTGGGCGGC GGCGGCAACA TGGTGGAGTA CGAGCGGACC 
    ACGCTGCGGG AGGAAGACGC ACCTGAGACC CCCGTAGAGG GCGGGGCCTC CCCGGACGCT
    GTGGAGGTGG GATTCCGGAA GAGGACAAGA GACTTCTTGG GCTCGCACAC CCAACTGGAG
    CTGGTCTTAG CCGCTGTCTC TCTACTGCTG GCTGCACTGC TACTGGGCTG CTTGGTGGCT
    CTGGGGGTCC AGTACCACGG GGACCCATCC CACAACACCT GCCTCACAGA GGCCTGCATT
    CGAGTAGCTG GAAAGATTCT GGAGTCCCTG GACCGCGGGG TGAGTCCCTG TGAGGACTTT
    TACCAGTTTT CCTGTGGTGG CTGGATTCAG AGAAATCCTC TGCCCGATGG GCGTTCTCGT
    TGGAACACCT TCAACAGCCT CTGGGACCAA AATCAGGCCA TACTGAAGCA CCTGCTTGAA
    AACACCACCT TCAACTCTAC CAGTGAAGCT GAGCGGAAGA CACAGCGTTT CTACCTCTCC
    TGCCTGCAGG TGGAGCGGAT CGAGGAACTG GGAGCCCAGC CACTGCGAGA CCTCATTGAC
    AAGATTGGTG GTTGGAACAT TACCGGACCC TGGGACCAGG ACAACTTCAT GGAAGTGCTG
    AAGGCAGTAG CAGGGACCTA CAGAGCTACT CCCTTCTTCA CTGTCTACGT CAGTGCCGAC
    TCTAAGAGCT CTAACAGCAA TGTTATCCAG GTGGACCAAT CTGGGCTCTT TCTCCCCTCT
    CGAGACTACT ACCTAAACAG GACCGCCAAT GAAAAAGTGC TTACCGCCTA CCTTGACTAC
    ATGGAGGAGC TGGGGATGTT GCTGGGTGGA CGGCCAACCT CCACACGGGA GCAGATGCGG
    CAGGTGCTGG AGCTGGAGAT ACAACTGGCC AACATCACAG TGCCCCAGGA CCAGAGGCGC
    GATGAGGAGA AGATCTATCA TAAGATGAAC ATTGCAGAGC TGCAGGCCCT GGCACCCTCT
    GTGGACTGGC TGGAGTTTCT GTCTTTCTTG TTGTCACCGC TGGAGCTGGG TGACTCTGAA
    CCTGTGGTGG TGTATGGAAC GGATTATTTG CAGCAGGTGT CAGAGCTCAT CAACCACACA
    GAGCCAAGTG TCCTGAACAA TTACCTGATT TGGAATCTGG TACAGAAGAC AACCTCAAGC
    CTGGACCACC GCTTTGAATC TGCACAGGAG AAACTGCTGG AGACTCTCTA TGGCACCAAG
    AAGAGCCGAG AGGGCTGGCC CCAGAGAAGA GAAAGGCCCT CAGACTGGAG AACAGAGCTA
    TCTCTGGGAG GCCTGGCTCT TTGTCCCTTA CAGTCCTGCA TGCCAAGGTG GCAGACCTGC
    ATCTCCAACA CGGACGACGC ACTTGGCTTC GCTTTGGGCT CCCTCTTTGT GAAGGCCACG
    TTCGATCGGC AAAGCAAGGA AATTGCTGAA GGGATGATCA GCGAGATCCG GAATGCCTTT
    GAGGAAGCCC TGGGGCAGCT GGTTTGGATG GATGAGAAGA CCCGCCAGGC AGCCAAGGAG
    AAAGCAGATG CTATATATGA TATGATTGGT TTCCCGGACT TCATCCTGGA TCCCAAAGAG
    CTGGATGATG TTTATGATGG GTACGAAGTC TCTGAAGATT CCTTCTTCCA GAACATGTTG
    AATTTGTACA ATTTCTCTGC TAAGGTGATG GCTGACCAGC TCCGCAAGCC TCCCAGCCGG
    GACCAGTGGA GCATGACCCC CCAGACAGTG AATGCCTACT ACCTTCCAAC CAAGAATGAA
    ATTGTCTTCC CTGCCGGCAT CTTGCAGGCC CCCTTCTACA CTCGTAACCA CCCCAAGGCC
    CTGAACTTTG GTGGAATCGG CGTGGTGATG GGCCATGAGT TGACACATGC GTTTGATGAC
    CAAGGTCGCG AATATGACAA GGAAGGGAAC CTGCGGCCCT GGTGGCAGAA TGAGTCCCTG
    GCAGCCTTCC GGAATCACAC AGCTTGCATG GAGGAACAGT ACAGCCAGTA CCAGGTCAAC
    GGGGAGAGGC TCAATGGGCG CCAGACGCTG GGGGAGAACA TCGCTGACAA CGGGGGGCTG
    AAGGCTGCCT ATAACGCTTA CAAAGCATGG CTGAGCAAGC ATGGGGAAGA GCAGCAGCTG
    CCAGCCGTGG GGCTCACCAA TCACCAGCTC TTCTTTGTGG GATTTGCCCA GGTGTGGTGC
    TCAGTCCGCA CACCCGAGAG CTCTCACGAG GGGCTGGTGA CTGACCCCCA CAGCCCCGCC
    CGCTTCCGCG TGCTGGGCAC TCTCTCCAAC TCCCGGGACT TCCTGCGACA CTTCGGCTGC
    CCTGTCGGCT CCCCCATGAA CCCAGGGCAG CTGTGTGAGG TGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965869.1
    CDS83..2470
    Translation

    Target ORF information:

    RefSeq Version XM_014110394.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris endothelin converting enzyme 2 (ECE2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014110394.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGAACGTCG CGCTGCAGGA GCTGGGCGGC GGCGGCAACA TGGTGGAGTA CGAGCGGACC 
    ACGCTGCGGG AGGAAGACGC ACCTGAGACC CCCGTAGAGG GCGGGGCCTC CCCGGACGCT
    GTGGAGGTGG GATTCCGGAA GAGGACAAGA GACTTCTTGG GCTCGCACAC CCAACTGGAG
    CTGGTCTTAG CCGCTGTCTC TCTACTGCTG GCTGCACTGC TACTGGGCTG CTTGGTGGCT
    CTGGGGGTCC AGTACCACGG GGACCCATCC CACAACACCT GCCTCACAGA GGCCTGCATT
    CGAGTAGCTG GAAAGATTCT GGAGTCCCTG GACCGCGGGG TGAGTCCCTG TGAGGACTTT
    TACCAGTTTT CCTGTGGTGG CTGGATTCAG AGAAATCCTC TGCCCGATGG GCGTTCTCGT
    TGGAACACCT TCAACAGCCT CTGGGACCAA AATCAGGCCA TACTGAAGCA CCTGCTTGAA
    AACACCACCT TCAACTCTAC CAGTGAAGCT GAGCGGAAGA CACAGCGTTT CTACCTCTCC
    TGCCTGCAGG TGGAGCGGAT CGAGGAACTG GGAGCCCAGC CACTGCGAGA CCTCATTGAC
    AAGATTGGTG GTTGGAACAT TACCGGACCC TGGGACCAGG ACAACTTCAT GGAAGTGCTG
    AAGGCAGTAG CAGGGACCTA CAGAGCTACT CCCTTCTTCA CTGTCTACGT CAGTGCCGAC
    TCTAAGAGCT CTAACAGCAA TGTTATCCAG GTGGACCAAT CTGGGCTCTT TCTCCCCTCT
    CGAGACTACT ACCTAAACAG GACCGCCAAT GAAAAAGTGC TTACCGCCTA CCTTGACTAC
    ATGGAGGAGC TGGGGATGTT GCTGGGTGGA CGGCCAACCT CCACACGGGA GCAGATGCGG
    CAGGTGCTGG AGCTGGAGAT ACAACTGGCC AACATCACAG TGCCCCAGGA CCAGAGGCGC
    GATGAGGAGA AGATCTATCA TAAGATGAAC ATTGCAGAGC TGCAGGCCCT GGCACCCTCT
    GTGGACTGGC TGGAGTTTCT GTCTTTCTTG TTGTCACCGC TGGAGCTGGG TGACTCTGAA
    CCTGTGGTGG TGTATGGAAC GGATTATTTG CAGCAGGTGT CAGAGCTCAT CAACCACACA
    GAGCCAAGTG TCCTGAACAA TTACCTGATT TGGAATCTGG TACAGAAGAC AACCTCAAGC
    CTGGACCACC GCTTTGAATC TGCACAGGAG AAACTGCTGG AGACTCTCTA TGGCACCAAG
    AAGAGCCGAG AGGGCTGGCC CCAGAGAAGA GAAAGGCCCT CAGACTGGAG AACAGAGCTA
    TCTCTGGGAG GCCTGGCTCT TTGTCCCTTA CAGTCCTGCA TGCCAAGGTG GCAGACCTGC
    ATCTCCAACA CGGACGACGC ACTTGGCTTC GCTTTGGGCT CCCTCTTTGT GAAGGCCACG
    TTCGATCGGC AAAGCAAGGA AATTGCTGAA GGGATGATCA GCGAGATCCG GAATGCCTTT
    GAGGAAGCCC TGGGGCAGCT GGTTTGGATG GATGAGAAGA CCCGCCAGGC AGCCAAGGAG
    AAAGCAGATG CTATATATGA TATGATTGGT TTCCCGGACT TCATCCTGGA TCCCAAAGAG
    CTGGATGATG TTTATGATGG GTACGAAGTC TCTGAAGATT CCTTCTTCCA GAACATGTTG
    AATTTGTACA ATTTCTCTGC TAAGGTGATG GCTGACCAGC TCCGCAAGCC TCCCAGCCGG
    GACCAGTGGA GCATGACCCC CCAGACAGTG AATGCCTACT ACCTTCCAAC CAAGAATGAA
    ATTGTCTTCC CTGCCGGCAT CTTGCAGGCC CCCTTCTACA CTCGTAACCA CCCCAAGGCC
    CTGAACTTTG GTGGAATCGG CGTGGTGATG GGCCATGAGT TGACACATGC GTTTGATGAC
    CAAGGTCGCG AATATGACAA GGAAGGGAAC CTGCGGCCCT GGTGGCAGAA TGAGTCCCTG
    GCAGCCTTCC GGAATCACAC AGCTTGCATG GAGGAACAGT ACAGCCAGTA CCAGGTCAAC
    GGGGAGAGGC TCAATGGGCG CCAGACGCTG GGGGAGAACA TCGCTGACAA CGGGGGGCTG
    AAGGCTGCCT ATAACGCTTA CAAAGCATGG CTGAGCAAGC ATGGGGAAGA GCAGCAGCTG
    CCAGCCGTGG GGCTCACCAA TCACCAGCTC TTCTTTGTGG GATTTGCCCA GGTGTGGTGC
    TCAGTCCGCA CACCCGAGAG CTCTCACGAG GGGCTGGTGA CTGACCCCCA CAGCCCCGCC
    CGCTTCCGCG TGCTGGGCAC TCTCTCCAAC TCCCGGGACT TCCTGCGACA CTTCGGCTGC
    CCTGTCGGCT CCCCCATGAA CCCAGGGCAG CTGTGTGAGG TGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44829
    Clone ID Related Accession (Same CDS sequence) XM_022414235.1 , XM_014110395.1
    Accession Version XM_022414235.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2349bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product endothelin-converting enzyme 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGTGGAGT ACGAGCGGAC CACGCTGCGG GAGGAAGACG CACCTGAGAC CCCCGTAGAG 
    GGCGGGGCCT CCCCGGACGC TGTGGAGGTG GGATTCCGGA AGAGGACAAG AGACTTCTTG
    GGCTCGCACA CCCAACTGGA GCTGGTCTTA GCCGCTGTCT CTCTACTGCT GGCTGCACTG
    CTACTGGGCT GCTTGGTGGC TCTGGGGGTC CAGTACCACG GGGACCCATC CCACAACACC
    TGCCTCACAG AGGCCTGCAT TCGAGTAGCT GGAAAGATTC TGGAGTCCCT GGACCGCGGG
    GTGAGTCCCT GTGAGGACTT TTACCAGTTT TCCTGTGGTG GCTGGATTCA GAGAAATCCT
    CTGCCCGATG GGCGTTCTCG TTGGAACACC TTCAACAGCC TCTGGGACCA AAATCAGGCC
    ATACTGAAGC ACCTGCTTGA AAACACCACC TTCAACTCTA CCAGTGAAGC TGAGCGGAAG
    ACACAGCGTT TCTACCTCTC CTGCCTGCAG GTGGAGCGGA TCGAGGAACT GGGAGCCCAG
    CCACTGCGAG ACCTCATTGA CAAGATTGGT GGTTGGAACA TTACCGGACC CTGGGACCAG
    GACAACTTCA TGGAAGTGCT GAAGGCAGTA GCAGGGACCT ACAGAGCTAC TCCCTTCTTC
    ACTGTCTACG TCAGTGCCGA CTCTAAGAGC TCTAACAGCA ATGTTATCCA GGTGGACCAA
    TCTGGGCTCT TTCTCCCCTC TCGAGACTAC TACCTAAACA GGACCGCCAA TGAAAAAGTG
    CTTACCGCCT ACCTTGACTA CATGGAGGAG CTGGGGATGT TGCTGGGTGG ACGGCCAACC
    TCCACACGGG AGCAGATGCG GCAGGTGCTG GAGCTGGAGA TACAACTGGC CAACATCACA
    GTGCCCCAGG ACCAGAGGCG CGATGAGGAG AAGATCTATC ATAAGATGAA CATTGCAGAG
    CTGCAGGCCC TGGCACCCTC TGTGGACTGG CTGGAGTTTC TGTCTTTCTT GTTGTCACCG
    CTGGAGCTGG GTGACTCTGA ACCTGTGGTG GTGTATGGAA CGGATTATTT GCAGCAGGTG
    TCAGAGCTCA TCAACCACAC AGAGCCAAGT GTCCTGAACA ATTACCTGAT TTGGAATCTG
    GTACAGAAGA CAACCTCAAG CCTGGACCAC CGCTTTGAAT CTGCACAGGA GAAACTGCTG
    GAGACTCTCT ATGGCACCAA GAAGAGCCGA GAGGGCTGGC CCCAGAGAAG AGAAAGGCCC
    TCAGACTGGA GAACAGAGCT ATCTCTGGGA GGCCTGGCTC TTTGTCCCTT ACAGTCCTGC
    ATGCCAAGGT GGCAGACCTG CATCTCCAAC ACGGACGACG CACTTGGCTT CGCTTTGGGC
    TCCCTCTTTG TGAAGGCCAC GTTCGATCGG CAAAGCAAGG AAATTGCTGA AGGGATGATC
    AGCGAGATCC GGAATGCCTT TGAGGAAGCC CTGGGGCAGC TGGTTTGGAT GGATGAGAAG
    ACCCGCCAGG CAGCCAAGGA GAAAGCAGAT GCTATATATG ATATGATTGG TTTCCCGGAC
    TTCATCCTGG ATCCCAAAGA GCTGGATGAT GTTTATGATG GGTACGAAGT CTCTGAAGAT
    TCCTTCTTCC AGAACATGTT GAATTTGTAC AATTTCTCTG CTAAGGTGAT GGCTGACCAG
    CTCCGCAAGC CTCCCAGCCG GGACCAGTGG AGCATGACCC CCCAGACAGT GAATGCCTAC
    TACCTTCCAA CCAAGAATGA AATTGTCTTC CCTGCCGGCA TCTTGCAGGC CCCCTTCTAC
    ACTCGTAACC ACCCCAAGGC CCTGAACTTT GGTGGAATCG GCGTGGTGAT GGGCCATGAG
    TTGACACATG CGTTTGATGA CCAAGGTCGC GAATATGACA AGGAAGGGAA CCTGCGGCCC
    TGGTGGCAGA ATGAGTCCCT GGCAGCCTTC CGGAATCACA CAGCTTGCAT GGAGGAACAG
    TACAGCCAGT ACCAGGTCAA CGGGGAGAGG CTCAATGGGC GCCAGACGCT GGGGGAGAAC
    ATCGCTGACA ACGGGGGGCT GAAGGCTGCC TATAACGCTT ACAAAGCATG GCTGAGCAAG
    CATGGGGAAG AGCAGCAGCT GCCAGCCGTG GGGCTCACCA ATCACCAGCT CTTCTTTGTG
    GGATTTGCCC AGGTGTGGTG CTCAGTCCGC ACACCCGAGA GCTCTCACGA GGGGCTGGTG
    ACTGACCCCC ACAGCCCCGC CCGCTTCCGC GTGCTGGGCA CTCTCTCCAA CTCCCGGGAC
    TTCCTGCGAC ACTTCGGCTG CCCTGTCGGC TCCCCCATGA ACCCAGGGCA GCTGTGTGAG
    GTGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022269943.1
    CDS177..2525
    Translation

    Target ORF information:

    RefSeq Version XM_022414235.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris endothelin converting enzyme 2 (ECE2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022414235.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGTGGAGT ACGAGCGGAC CACGCTGCGG GAGGAAGACG CACCTGAGAC CCCCGTAGAG 
    GGCGGGGCCT CCCCGGACGC TGTGGAGGTG GGATTCCGGA AGAGGACAAG AGACTTCTTG
    GGCTCGCACA CCCAACTGGA GCTGGTCTTA GCCGCTGTCT CTCTACTGCT GGCTGCACTG
    CTACTGGGCT GCTTGGTGGC TCTGGGGGTC CAGTACCACG GGGACCCATC CCACAACACC
    TGCCTCACAG AGGCCTGCAT TCGAGTAGCT GGAAAGATTC TGGAGTCCCT GGACCGCGGG
    GTGAGTCCCT GTGAGGACTT TTACCAGTTT TCCTGTGGTG GCTGGATTCA GAGAAATCCT
    CTGCCCGATG GGCGTTCTCG TTGGAACACC TTCAACAGCC TCTGGGACCA AAATCAGGCC
    ATACTGAAGC ACCTGCTTGA AAACACCACC TTCAACTCTA CCAGTGAAGC TGAGCGGAAG
    ACACAGCGTT TCTACCTCTC CTGCCTGCAG GTGGAGCGGA TCGAGGAACT GGGAGCCCAG
    CCACTGCGAG ACCTCATTGA CAAGATTGGT GGTTGGAACA TTACCGGACC CTGGGACCAG
    GACAACTTCA TGGAAGTGCT GAAGGCAGTA GCAGGGACCT ACAGAGCTAC TCCCTTCTTC
    ACTGTCTACG TCAGTGCCGA CTCTAAGAGC TCTAACAGCA ATGTTATCCA GGTGGACCAA
    TCTGGGCTCT TTCTCCCCTC TCGAGACTAC TACCTAAACA GGACCGCCAA TGAAAAAGTG
    CTTACCGCCT ACCTTGACTA CATGGAGGAG CTGGGGATGT TGCTGGGTGG ACGGCCAACC
    TCCACACGGG AGCAGATGCG GCAGGTGCTG GAGCTGGAGA TACAACTGGC CAACATCACA
    GTGCCCCAGG ACCAGAGGCG CGATGAGGAG AAGATCTATC ATAAGATGAA CATTGCAGAG
    CTGCAGGCCC TGGCACCCTC TGTGGACTGG CTGGAGTTTC TGTCTTTCTT GTTGTCACCG
    CTGGAGCTGG GTGACTCTGA ACCTGTGGTG GTGTATGGAA CGGATTATTT GCAGCAGGTG
    TCAGAGCTCA TCAACCACAC AGAGCCAAGT GTCCTGAACA ATTACCTGAT TTGGAATCTG
    GTACAGAAGA CAACCTCAAG CCTGGACCAC CGCTTTGAAT CTGCACAGGA GAAACTGCTG
    GAGACTCTCT ATGGCACCAA GAAGAGCCGA GAGGGCTGGC CCCAGAGAAG AGAAAGGCCC
    TCAGACTGGA GAACAGAGCT ATCTCTGGGA GGCCTGGCTC TTTGTCCCTT ACAGTCCTGC
    ATGCCAAGGT GGCAGACCTG CATCTCCAAC ACGGACGACG CACTTGGCTT CGCTTTGGGC
    TCCCTCTTTG TGAAGGCCAC GTTCGATCGG CAAAGCAAGG AAATTGCTGA AGGGATGATC
    AGCGAGATCC GGAATGCCTT TGAGGAAGCC CTGGGGCAGC TGGTTTGGAT GGATGAGAAG
    ACCCGCCAGG CAGCCAAGGA GAAAGCAGAT GCTATATATG ATATGATTGG TTTCCCGGAC
    TTCATCCTGG ATCCCAAAGA GCTGGATGAT GTTTATGATG GGTACGAAGT CTCTGAAGAT
    TCCTTCTTCC AGAACATGTT GAATTTGTAC AATTTCTCTG CTAAGGTGAT GGCTGACCAG
    CTCCGCAAGC CTCCCAGCCG GGACCAGTGG AGCATGACCC CCCAGACAGT GAATGCCTAC
    TACCTTCCAA CCAAGAATGA AATTGTCTTC CCTGCCGGCA TCTTGCAGGC CCCCTTCTAC
    ACTCGTAACC ACCCCAAGGC CCTGAACTTT GGTGGAATCG GCGTGGTGAT GGGCCATGAG
    TTGACACATG CGTTTGATGA CCAAGGTCGC GAATATGACA AGGAAGGGAA CCTGCGGCCC
    TGGTGGCAGA ATGAGTCCCT GGCAGCCTTC CGGAATCACA CAGCTTGCAT GGAGGAACAG
    TACAGCCAGT ACCAGGTCAA CGGGGAGAGG CTCAATGGGC GCCAGACGCT GGGGGAGAAC
    ATCGCTGACA ACGGGGGGCT GAAGGCTGCC TATAACGCTT ACAAAGCATG GCTGAGCAAG
    CATGGGGAAG AGCAGCAGCT GCCAGCCGTG GGGCTCACCA ATCACCAGCT CTTCTTTGTG
    GGATTTGCCC AGGTGTGGTG CTCAGTCCGC ACACCCGAGA GCTCTCACGA GGGGCTGGTG
    ACTGACCCCC ACAGCCCCGC CCGCTTCCGC GTGCTGGGCA CTCTCTCCAA CTCCCGGGAC
    TTCCTGCGAC ACTTCGGCTG CCCTGTCGGC TCCCCCATGA ACCCAGGGCA GCTGTGTGAG
    GTGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44829
    Clone ID Related Accession (Same CDS sequence) XM_022414235.1 , XM_014110395.1
    Accession Version XM_014110395.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2349bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product endothelin-converting enzyme 2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726120.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGTGGAGT ACGAGCGGAC CACGCTGCGG GAGGAAGACG CACCTGAGAC CCCCGTAGAG 
    GGCGGGGCCT CCCCGGACGC TGTGGAGGTG GGATTCCGGA AGAGGACAAG AGACTTCTTG
    GGCTCGCACA CCCAACTGGA GCTGGTCTTA GCCGCTGTCT CTCTACTGCT GGCTGCACTG
    CTACTGGGCT GCTTGGTGGC TCTGGGGGTC CAGTACCACG GGGACCCATC CCACAACACC
    TGCCTCACAG AGGCCTGCAT TCGAGTAGCT GGAAAGATTC TGGAGTCCCT GGACCGCGGG
    GTGAGTCCCT GTGAGGACTT TTACCAGTTT TCCTGTGGTG GCTGGATTCA GAGAAATCCT
    CTGCCCGATG GGCGTTCTCG TTGGAACACC TTCAACAGCC TCTGGGACCA AAATCAGGCC
    ATACTGAAGC ACCTGCTTGA AAACACCACC TTCAACTCTA CCAGTGAAGC TGAGCGGAAG
    ACACAGCGTT TCTACCTCTC CTGCCTGCAG GTGGAGCGGA TCGAGGAACT GGGAGCCCAG
    CCACTGCGAG ACCTCATTGA CAAGATTGGT GGTTGGAACA TTACCGGACC CTGGGACCAG
    GACAACTTCA TGGAAGTGCT GAAGGCAGTA GCAGGGACCT ACAGAGCTAC TCCCTTCTTC
    ACTGTCTACG TCAGTGCCGA CTCTAAGAGC TCTAACAGCA ATGTTATCCA GGTGGACCAA
    TCTGGGCTCT TTCTCCCCTC TCGAGACTAC TACCTAAACA GGACCGCCAA TGAAAAAGTG
    CTTACCGCCT ACCTTGACTA CATGGAGGAG CTGGGGATGT TGCTGGGTGG ACGGCCAACC
    TCCACACGGG AGCAGATGCG GCAGGTGCTG GAGCTGGAGA TACAACTGGC CAACATCACA
    GTGCCCCAGG ACCAGAGGCG CGATGAGGAG AAGATCTATC ATAAGATGAA CATTGCAGAG
    CTGCAGGCCC TGGCACCCTC TGTGGACTGG CTGGAGTTTC TGTCTTTCTT GTTGTCACCG
    CTGGAGCTGG GTGACTCTGA ACCTGTGGTG GTGTATGGAA CGGATTATTT GCAGCAGGTG
    TCAGAGCTCA TCAACCACAC AGAGCCAAGT GTCCTGAACA ATTACCTGAT TTGGAATCTG
    GTACAGAAGA CAACCTCAAG CCTGGACCAC CGCTTTGAAT CTGCACAGGA GAAACTGCTG
    GAGACTCTCT ATGGCACCAA GAAGAGCCGA GAGGGCTGGC CCCAGAGAAG AGAAAGGCCC
    TCAGACTGGA GAACAGAGCT ATCTCTGGGA GGCCTGGCTC TTTGTCCCTT ACAGTCCTGC
    ATGCCAAGGT GGCAGACCTG CATCTCCAAC ACGGACGACG CACTTGGCTT CGCTTTGGGC
    TCCCTCTTTG TGAAGGCCAC GTTCGATCGG CAAAGCAAGG AAATTGCTGA AGGGATGATC
    AGCGAGATCC GGAATGCCTT TGAGGAAGCC CTGGGGCAGC TGGTTTGGAT GGATGAGAAG
    ACCCGCCAGG CAGCCAAGGA GAAAGCAGAT GCTATATATG ATATGATTGG TTTCCCGGAC
    TTCATCCTGG ATCCCAAAGA GCTGGATGAT GTTTATGATG GGTACGAAGT CTCTGAAGAT
    TCCTTCTTCC AGAACATGTT GAATTTGTAC AATTTCTCTG CTAAGGTGAT GGCTGACCAG
    CTCCGCAAGC CTCCCAGCCG GGACCAGTGG AGCATGACCC CCCAGACAGT GAATGCCTAC
    TACCTTCCAA CCAAGAATGA AATTGTCTTC CCTGCCGGCA TCTTGCAGGC CCCCTTCTAC
    ACTCGTAACC ACCCCAAGGC CCTGAACTTT GGTGGAATCG GCGTGGTGAT GGGCCATGAG
    TTGACACATG CGTTTGATGA CCAAGGTCGC GAATATGACA AGGAAGGGAA CCTGCGGCCC
    TGGTGGCAGA ATGAGTCCCT GGCAGCCTTC CGGAATCACA CAGCTTGCAT GGAGGAACAG
    TACAGCCAGT ACCAGGTCAA CGGGGAGAGG CTCAATGGGC GCCAGACGCT GGGGGAGAAC
    ATCGCTGACA ACGGGGGGCT GAAGGCTGCC TATAACGCTT ACAAAGCATG GCTGAGCAAG
    CATGGGGAAG AGCAGCAGCT GCCAGCCGTG GGGCTCACCA ATCACCAGCT CTTCTTTGTG
    GGATTTGCCC AGGTGTGGTG CTCAGTCCGC ACACCCGAGA GCTCTCACGA GGGGCTGGTG
    ACTGACCCCC ACAGCCCCGC CCGCTTCCGC GTGCTGGGCA CTCTCTCCAA CTCCCGGGAC
    TTCCTGCGAC ACTTCGGCTG CCCTGTCGGC TCCCCCATGA ACCCAGGGCA GCTGTGTGAG
    GTGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965870.1
    CDS257..2605
    Translation

    Target ORF information:

    RefSeq Version XM_014110395.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris endothelin converting enzyme 2 (ECE2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014110395.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGTGGAGT ACGAGCGGAC CACGCTGCGG GAGGAAGACG CACCTGAGAC CCCCGTAGAG 
    GGCGGGGCCT CCCCGGACGC TGTGGAGGTG GGATTCCGGA AGAGGACAAG AGACTTCTTG
    GGCTCGCACA CCCAACTGGA GCTGGTCTTA GCCGCTGTCT CTCTACTGCT GGCTGCACTG
    CTACTGGGCT GCTTGGTGGC TCTGGGGGTC CAGTACCACG GGGACCCATC CCACAACACC
    TGCCTCACAG AGGCCTGCAT TCGAGTAGCT GGAAAGATTC TGGAGTCCCT GGACCGCGGG
    GTGAGTCCCT GTGAGGACTT TTACCAGTTT TCCTGTGGTG GCTGGATTCA GAGAAATCCT
    CTGCCCGATG GGCGTTCTCG TTGGAACACC TTCAACAGCC TCTGGGACCA AAATCAGGCC
    ATACTGAAGC ACCTGCTTGA AAACACCACC TTCAACTCTA CCAGTGAAGC TGAGCGGAAG
    ACACAGCGTT TCTACCTCTC CTGCCTGCAG GTGGAGCGGA TCGAGGAACT GGGAGCCCAG
    CCACTGCGAG ACCTCATTGA CAAGATTGGT GGTTGGAACA TTACCGGACC CTGGGACCAG
    GACAACTTCA TGGAAGTGCT GAAGGCAGTA GCAGGGACCT ACAGAGCTAC TCCCTTCTTC
    ACTGTCTACG TCAGTGCCGA CTCTAAGAGC TCTAACAGCA ATGTTATCCA GGTGGACCAA
    TCTGGGCTCT TTCTCCCCTC TCGAGACTAC TACCTAAACA GGACCGCCAA TGAAAAAGTG
    CTTACCGCCT ACCTTGACTA CATGGAGGAG CTGGGGATGT TGCTGGGTGG ACGGCCAACC
    TCCACACGGG AGCAGATGCG GCAGGTGCTG GAGCTGGAGA TACAACTGGC CAACATCACA
    GTGCCCCAGG ACCAGAGGCG CGATGAGGAG AAGATCTATC ATAAGATGAA CATTGCAGAG
    CTGCAGGCCC TGGCACCCTC TGTGGACTGG CTGGAGTTTC TGTCTTTCTT GTTGTCACCG
    CTGGAGCTGG GTGACTCTGA ACCTGTGGTG GTGTATGGAA CGGATTATTT GCAGCAGGTG
    TCAGAGCTCA TCAACCACAC AGAGCCAAGT GTCCTGAACA ATTACCTGAT TTGGAATCTG
    GTACAGAAGA CAACCTCAAG CCTGGACCAC CGCTTTGAAT CTGCACAGGA GAAACTGCTG
    GAGACTCTCT ATGGCACCAA GAAGAGCCGA GAGGGCTGGC CCCAGAGAAG AGAAAGGCCC
    TCAGACTGGA GAACAGAGCT ATCTCTGGGA GGCCTGGCTC TTTGTCCCTT ACAGTCCTGC
    ATGCCAAGGT GGCAGACCTG CATCTCCAAC ACGGACGACG CACTTGGCTT CGCTTTGGGC
    TCCCTCTTTG TGAAGGCCAC GTTCGATCGG CAAAGCAAGG AAATTGCTGA AGGGATGATC
    AGCGAGATCC GGAATGCCTT TGAGGAAGCC CTGGGGCAGC TGGTTTGGAT GGATGAGAAG
    ACCCGCCAGG CAGCCAAGGA GAAAGCAGAT GCTATATATG ATATGATTGG TTTCCCGGAC
    TTCATCCTGG ATCCCAAAGA GCTGGATGAT GTTTATGATG GGTACGAAGT CTCTGAAGAT
    TCCTTCTTCC AGAACATGTT GAATTTGTAC AATTTCTCTG CTAAGGTGAT GGCTGACCAG
    CTCCGCAAGC CTCCCAGCCG GGACCAGTGG AGCATGACCC CCCAGACAGT GAATGCCTAC
    TACCTTCCAA CCAAGAATGA AATTGTCTTC CCTGCCGGCA TCTTGCAGGC CCCCTTCTAC
    ACTCGTAACC ACCCCAAGGC CCTGAACTTT GGTGGAATCG GCGTGGTGAT GGGCCATGAG
    TTGACACATG CGTTTGATGA CCAAGGTCGC GAATATGACA AGGAAGGGAA CCTGCGGCCC
    TGGTGGCAGA ATGAGTCCCT GGCAGCCTTC CGGAATCACA CAGCTTGCAT GGAGGAACAG
    TACAGCCAGT ACCAGGTCAA CGGGGAGAGG CTCAATGGGC GCCAGACGCT GGGGGAGAAC
    ATCGCTGACA ACGGGGGGCT GAAGGCTGCC TATAACGCTT ACAAAGCATG GCTGAGCAAG
    CATGGGGAAG AGCAGCAGCT GCCAGCCGTG GGGCTCACCA ATCACCAGCT CTTCTTTGTG
    GGATTTGCCC AGGTGTGGTG CTCAGTCCGC ACACCCGAGA GCTCTCACGA GGGGCTGGTG
    ACTGACCCCC ACAGCCCCGC CCGCTTCCGC GTGCTGGGCA CTCTCTCCAA CTCCCGGGAC
    TTCCTGCGAC ACTTCGGCTG CCCTGTCGGC TCCCCCATGA ACCCAGGGCA GCTGTGTGAG
    GTGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44830
    Clone ID Related Accession (Same CDS sequence) XM_014110396.2 , XM_014110396.1
    Accession Version XM_014110396.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2301bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product endothelin-converting enzyme 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014110396.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAACGTCG CGCTGCAGGA GCTGGGCGGC GGCGGCAACG TGGGATTCCG GAAGAGGACA 
    AGAGACTTCT TGGGCTCGCA CACCCAACTG GAGCTGGTCT TAGCCGCTGT CTCTCTACTG
    CTGGCTGCAC TGCTACTGGG CTGCTTGGTG GCTCTGGGGG TCCAGTACCA CGGGGACCCA
    TCCCACAACA CCTGCCTCAC AGAGGCCTGC ATTCGAGTAG CTGGAAAGAT TCTGGAGTCC
    CTGGACCGCG GGGTGAGTCC CTGTGAGGAC TTTTACCAGT TTTCCTGTGG TGGCTGGATT
    CAGAGAAATC CTCTGCCCGA TGGGCGTTCT CGTTGGAACA CCTTCAACAG CCTCTGGGAC
    CAAAATCAGG CCATACTGAA GCACCTGCTT GAAAACACCA CCTTCAACTC TACCAGTGAA
    GCTGAGCGGA AGACACAGCG TTTCTACCTC TCCTGCCTGC AGGTGGAGCG GATCGAGGAA
    CTGGGAGCCC AGCCACTGCG AGACCTCATT GACAAGATTG GTGGTTGGAA CATTACCGGA
    CCCTGGGACC AGGACAACTT CATGGAAGTG CTGAAGGCAG TAGCAGGGAC CTACAGAGCT
    ACTCCCTTCT TCACTGTCTA CGTCAGTGCC GACTCTAAGA GCTCTAACAG CAATGTTATC
    CAGGTGGACC AATCTGGGCT CTTTCTCCCC TCTCGAGACT ACTACCTAAA CAGGACCGCC
    AATGAAAAAG TGCTTACCGC CTACCTTGAC TACATGGAGG AGCTGGGGAT GTTGCTGGGT
    GGACGGCCAA CCTCCACACG GGAGCAGATG CGGCAGGTGC TGGAGCTGGA GATACAACTG
    GCCAACATCA CAGTGCCCCA GGACCAGAGG CGCGATGAGG AGAAGATCTA TCATAAGATG
    AACATTGCAG AGCTGCAGGC CCTGGCACCC TCTGTGGACT GGCTGGAGTT TCTGTCTTTC
    TTGTTGTCAC CGCTGGAGCT GGGTGACTCT GAACCTGTGG TGGTGTATGG AACGGATTAT
    TTGCAGCAGG TGTCAGAGCT CATCAACCAC ACAGAGCCAA GTGTCCTGAA CAATTACCTG
    ATTTGGAATC TGGTACAGAA GACAACCTCA AGCCTGGACC ACCGCTTTGA ATCTGCACAG
    GAGAAACTGC TGGAGACTCT CTATGGCACC AAGAAGAGCC GAGAGGGCTG GCCCCAGAGA
    AGAGAAAGGC CCTCAGACTG GAGAACAGAG CTATCTCTGG GAGGCCTGGC TCTTTGTCCC
    TTACAGTCCT GCATGCCAAG GTGGCAGACC TGCATCTCCA ACACGGACGA CGCACTTGGC
    TTCGCTTTGG GCTCCCTCTT TGTGAAGGCC ACGTTCGATC GGCAAAGCAA GGAAATTGCT
    GAAGGGATGA TCAGCGAGAT CCGGAATGCC TTTGAGGAAG CCCTGGGGCA GCTGGTTTGG
    ATGGATGAGA AGACCCGCCA GGCAGCCAAG GAGAAAGCAG ATGCTATATA TGATATGATT
    GGTTTCCCGG ACTTCATCCT GGATCCCAAA GAGCTGGATG ATGTTTATGA TGGGTACGAA
    GTCTCTGAAG ATTCCTTCTT CCAGAACATG TTGAATTTGT ACAATTTCTC TGCTAAGGTG
    ATGGCTGACC AGCTCCGCAA GCCTCCCAGC CGGGACCAGT GGAGCATGAC CCCCCAGACA
    GTGAATGCCT ACTACCTTCC AACCAAGAAT GAAATTGTCT TCCCTGCCGG CATCTTGCAG
    GCCCCCTTCT ACACTCGTAA CCACCCCAAG GCCCTGAACT TTGGTGGAAT CGGCGTGGTG
    ATGGGCCATG AGTTGACACA TGCGTTTGAT GACCAAGGTC GCGAATATGA CAAGGAAGGG
    AACCTGCGGC CCTGGTGGCA GAATGAGTCC CTGGCAGCCT TCCGGAATCA CACAGCTTGC
    ATGGAGGAAC AGTACAGCCA GTACCAGGTC AACGGGGAGA GGCTCAATGG GCGCCAGACG
    CTGGGGGAGA ACATCGCTGA CAACGGGGGG CTGAAGGCTG CCTATAACGC TTACAAAGCA
    TGGCTGAGCA AGCATGGGGA AGAGCAGCAG CTGCCAGCCG TGGGGCTCAC CAATCACCAG
    CTCTTCTTTG TGGGATTTGC CCAGGTGTGG TGCTCAGTCC GCACACCCGA GAGCTCTCAC
    GAGGGGCTGG TGACTGACCC CCACAGCCCC GCCCGCTTCC GCGTGCTGGG CACTCTCTCC
    AACTCCCGGG ACTTCCTGCG ACACTTCGGC TGCCCTGTCG GCTCCCCCAT GAACCCAGGG
    CAGCTGTGTG AGGTGTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965871.1
    CDS83..2383
    Translation

    Target ORF information:

    RefSeq Version XM_014110396.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris endothelin converting enzyme 2 (ECE2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014110396.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAACGTCG CGCTGCAGGA GCTGGGCGGC GGCGGCAACG TGGGATTCCG GAAGAGGACA 
    AGAGACTTCT TGGGCTCGCA CACCCAACTG GAGCTGGTCT TAGCCGCTGT CTCTCTACTG
    CTGGCTGCAC TGCTACTGGG CTGCTTGGTG GCTCTGGGGG TCCAGTACCA CGGGGACCCA
    TCCCACAACA CCTGCCTCAC AGAGGCCTGC ATTCGAGTAG CTGGAAAGAT TCTGGAGTCC
    CTGGACCGCG GGGTGAGTCC CTGTGAGGAC TTTTACCAGT TTTCCTGTGG TGGCTGGATT
    CAGAGAAATC CTCTGCCCGA TGGGCGTTCT CGTTGGAACA CCTTCAACAG CCTCTGGGAC
    CAAAATCAGG CCATACTGAA GCACCTGCTT GAAAACACCA CCTTCAACTC TACCAGTGAA
    GCTGAGCGGA AGACACAGCG TTTCTACCTC TCCTGCCTGC AGGTGGAGCG GATCGAGGAA
    CTGGGAGCCC AGCCACTGCG AGACCTCATT GACAAGATTG GTGGTTGGAA CATTACCGGA
    CCCTGGGACC AGGACAACTT CATGGAAGTG CTGAAGGCAG TAGCAGGGAC CTACAGAGCT
    ACTCCCTTCT TCACTGTCTA CGTCAGTGCC GACTCTAAGA GCTCTAACAG CAATGTTATC
    CAGGTGGACC AATCTGGGCT CTTTCTCCCC TCTCGAGACT ACTACCTAAA CAGGACCGCC
    AATGAAAAAG TGCTTACCGC CTACCTTGAC TACATGGAGG AGCTGGGGAT GTTGCTGGGT
    GGACGGCCAA CCTCCACACG GGAGCAGATG CGGCAGGTGC TGGAGCTGGA GATACAACTG
    GCCAACATCA CAGTGCCCCA GGACCAGAGG CGCGATGAGG AGAAGATCTA TCATAAGATG
    AACATTGCAG AGCTGCAGGC CCTGGCACCC TCTGTGGACT GGCTGGAGTT TCTGTCTTTC
    TTGTTGTCAC CGCTGGAGCT GGGTGACTCT GAACCTGTGG TGGTGTATGG AACGGATTAT
    TTGCAGCAGG TGTCAGAGCT CATCAACCAC ACAGAGCCAA GTGTCCTGAA CAATTACCTG
    ATTTGGAATC TGGTACAGAA GACAACCTCA AGCCTGGACC ACCGCTTTGA ATCTGCACAG
    GAGAAACTGC TGGAGACTCT CTATGGCACC AAGAAGAGCC GAGAGGGCTG GCCCCAGAGA
    AGAGAAAGGC CCTCAGACTG GAGAACAGAG CTATCTCTGG GAGGCCTGGC TCTTTGTCCC
    TTACAGTCCT GCATGCCAAG GTGGCAGACC TGCATCTCCA ACACGGACGA CGCACTTGGC
    TTCGCTTTGG GCTCCCTCTT TGTGAAGGCC ACGTTCGATC GGCAAAGCAA GGAAATTGCT
    GAAGGGATGA TCAGCGAGAT CCGGAATGCC TTTGAGGAAG CCCTGGGGCA GCTGGTTTGG
    ATGGATGAGA AGACCCGCCA GGCAGCCAAG GAGAAAGCAG ATGCTATATA TGATATGATT
    GGTTTCCCGG ACTTCATCCT GGATCCCAAA GAGCTGGATG ATGTTTATGA TGGGTACGAA
    GTCTCTGAAG ATTCCTTCTT CCAGAACATG TTGAATTTGT ACAATTTCTC TGCTAAGGTG
    ATGGCTGACC AGCTCCGCAA GCCTCCCAGC CGGGACCAGT GGAGCATGAC CCCCCAGACA
    GTGAATGCCT ACTACCTTCC AACCAAGAAT GAAATTGTCT TCCCTGCCGG CATCTTGCAG
    GCCCCCTTCT ACACTCGTAA CCACCCCAAG GCCCTGAACT TTGGTGGAAT CGGCGTGGTG
    ATGGGCCATG AGTTGACACA TGCGTTTGAT GACCAAGGTC GCGAATATGA CAAGGAAGGG
    AACCTGCGGC CCTGGTGGCA GAATGAGTCC CTGGCAGCCT TCCGGAATCA CACAGCTTGC
    ATGGAGGAAC AGTACAGCCA GTACCAGGTC AACGGGGAGA GGCTCAATGG GCGCCAGACG
    CTGGGGGAGA ACATCGCTGA CAACGGGGGG CTGAAGGCTG CCTATAACGC TTACAAAGCA
    TGGCTGAGCA AGCATGGGGA AGAGCAGCAG CTGCCAGCCG TGGGGCTCAC CAATCACCAG
    CTCTTCTTTG TGGGATTTGC CCAGGTGTGG TGCTCAGTCC GCACACCCGA GAGCTCTCAC
    GAGGGGCTGG TGACTGACCC CCACAGCCCC GCCCGCTTCC GCGTGCTGGG CACTCTCTCC
    AACTCCCGGG ACTTCCTGCG ACACTTCGGC TGCCCTGTCG GCTCCCCCAT GAACCCAGGG
    CAGCTGTGTG AGGTGTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44830
    Clone ID Related Accession (Same CDS sequence) XM_014110396.2 , XM_014110396.1
    Accession Version XM_014110396.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2301bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product endothelin-converting enzyme 2 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726120.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAACGTCG CGCTGCAGGA GCTGGGCGGC GGCGGCAACG TGGGATTCCG GAAGAGGACA 
    AGAGACTTCT TGGGCTCGCA CACCCAACTG GAGCTGGTCT TAGCCGCTGT CTCTCTACTG
    CTGGCTGCAC TGCTACTGGG CTGCTTGGTG GCTCTGGGGG TCCAGTACCA CGGGGACCCA
    TCCCACAACA CCTGCCTCAC AGAGGCCTGC ATTCGAGTAG CTGGAAAGAT TCTGGAGTCC
    CTGGACCGCG GGGTGAGTCC CTGTGAGGAC TTTTACCAGT TTTCCTGTGG TGGCTGGATT
    CAGAGAAATC CTCTGCCCGA TGGGCGTTCT CGTTGGAACA CCTTCAACAG CCTCTGGGAC
    CAAAATCAGG CCATACTGAA GCACCTGCTT GAAAACACCA CCTTCAACTC TACCAGTGAA
    GCTGAGCGGA AGACACAGCG TTTCTACCTC TCCTGCCTGC AGGTGGAGCG GATCGAGGAA
    CTGGGAGCCC AGCCACTGCG AGACCTCATT GACAAGATTG GTGGTTGGAA CATTACCGGA
    CCCTGGGACC AGGACAACTT CATGGAAGTG CTGAAGGCAG TAGCAGGGAC CTACAGAGCT
    ACTCCCTTCT TCACTGTCTA CGTCAGTGCC GACTCTAAGA GCTCTAACAG CAATGTTATC
    CAGGTGGACC AATCTGGGCT CTTTCTCCCC TCTCGAGACT ACTACCTAAA CAGGACCGCC
    AATGAAAAAG TGCTTACCGC CTACCTTGAC TACATGGAGG AGCTGGGGAT GTTGCTGGGT
    GGACGGCCAA CCTCCACACG GGAGCAGATG CGGCAGGTGC TGGAGCTGGA GATACAACTG
    GCCAACATCA CAGTGCCCCA GGACCAGAGG CGCGATGAGG AGAAGATCTA TCATAAGATG
    AACATTGCAG AGCTGCAGGC CCTGGCACCC TCTGTGGACT GGCTGGAGTT TCTGTCTTTC
    TTGTTGTCAC CGCTGGAGCT GGGTGACTCT GAACCTGTGG TGGTGTATGG AACGGATTAT
    TTGCAGCAGG TGTCAGAGCT CATCAACCAC ACAGAGCCAA GTGTCCTGAA CAATTACCTG
    ATTTGGAATC TGGTACAGAA GACAACCTCA AGCCTGGACC ACCGCTTTGA ATCTGCACAG
    GAGAAACTGC TGGAGACTCT CTATGGCACC AAGAAGAGCC GAGAGGGCTG GCCCCAGAGA
    AGAGAAAGGC CCTCAGACTG GAGAACAGAG CTATCTCTGG GAGGCCTGGC TCTTTGTCCC
    TTACAGTCCT GCATGCCAAG GTGGCAGACC TGCATCTCCA ACACGGACGA CGCACTTGGC
    TTCGCTTTGG GCTCCCTCTT TGTGAAGGCC ACGTTCGATC GGCAAAGCAA GGAAATTGCT
    GAAGGGATGA TCAGCGAGAT CCGGAATGCC TTTGAGGAAG CCCTGGGGCA GCTGGTTTGG
    ATGGATGAGA AGACCCGCCA GGCAGCCAAG GAGAAAGCAG ATGCTATATA TGATATGATT
    GGTTTCCCGG ACTTCATCCT GGATCCCAAA GAGCTGGATG ATGTTTATGA TGGGTACGAA
    GTCTCTGAAG ATTCCTTCTT CCAGAACATG TTGAATTTGT ACAATTTCTC TGCTAAGGTG
    ATGGCTGACC AGCTCCGCAA GCCTCCCAGC CGGGACCAGT GGAGCATGAC CCCCCAGACA
    GTGAATGCCT ACTACCTTCC AACCAAGAAT GAAATTGTCT TCCCTGCCGG CATCTTGCAG
    GCCCCCTTCT ACACTCGTAA CCACCCCAAG GCCCTGAACT TTGGTGGAAT CGGCGTGGTG
    ATGGGCCATG AGTTGACACA TGCGTTTGAT GACCAAGGTC GCGAATATGA CAAGGAAGGG
    AACCTGCGGC CCTGGTGGCA GAATGAGTCC CTGGCAGCCT TCCGGAATCA CACAGCTTGC
    ATGGAGGAAC AGTACAGCCA GTACCAGGTC AACGGGGAGA GGCTCAATGG GCGCCAGACG
    CTGGGGGAGA ACATCGCTGA CAACGGGGGG CTGAAGGCTG CCTATAACGC TTACAAAGCA
    TGGCTGAGCA AGCATGGGGA AGAGCAGCAG CTGCCAGCCG TGGGGCTCAC CAATCACCAG
    CTCTTCTTTG TGGGATTTGC CCAGGTGTGG TGCTCAGTCC GCACACCCGA GAGCTCTCAC
    GAGGGGCTGG TGACTGACCC CCACAGCCCC GCCCGCTTCC GCGTGCTGGG CACTCTCTCC
    AACTCCCGGG ACTTCCTGCG ACACTTCGGC TGCCCTGTCG GCTCCCCCAT GAACCCAGGG
    CAGCTGTGTG AGGTGTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965871.1
    CDS83..2383
    Translation

    Target ORF information:

    RefSeq Version XM_014110396.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris endothelin converting enzyme 2 (ECE2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014110396.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAACGTCG CGCTGCAGGA GCTGGGCGGC GGCGGCAACG TGGGATTCCG GAAGAGGACA 
    AGAGACTTCT TGGGCTCGCA CACCCAACTG GAGCTGGTCT TAGCCGCTGT CTCTCTACTG
    CTGGCTGCAC TGCTACTGGG CTGCTTGGTG GCTCTGGGGG TCCAGTACCA CGGGGACCCA
    TCCCACAACA CCTGCCTCAC AGAGGCCTGC ATTCGAGTAG CTGGAAAGAT TCTGGAGTCC
    CTGGACCGCG GGGTGAGTCC CTGTGAGGAC TTTTACCAGT TTTCCTGTGG TGGCTGGATT
    CAGAGAAATC CTCTGCCCGA TGGGCGTTCT CGTTGGAACA CCTTCAACAG CCTCTGGGAC
    CAAAATCAGG CCATACTGAA GCACCTGCTT GAAAACACCA CCTTCAACTC TACCAGTGAA
    GCTGAGCGGA AGACACAGCG TTTCTACCTC TCCTGCCTGC AGGTGGAGCG GATCGAGGAA
    CTGGGAGCCC AGCCACTGCG AGACCTCATT GACAAGATTG GTGGTTGGAA CATTACCGGA
    CCCTGGGACC AGGACAACTT CATGGAAGTG CTGAAGGCAG TAGCAGGGAC CTACAGAGCT
    ACTCCCTTCT TCACTGTCTA CGTCAGTGCC GACTCTAAGA GCTCTAACAG CAATGTTATC
    CAGGTGGACC AATCTGGGCT CTTTCTCCCC TCTCGAGACT ACTACCTAAA CAGGACCGCC
    AATGAAAAAG TGCTTACCGC CTACCTTGAC TACATGGAGG AGCTGGGGAT GTTGCTGGGT
    GGACGGCCAA CCTCCACACG GGAGCAGATG CGGCAGGTGC TGGAGCTGGA GATACAACTG
    GCCAACATCA CAGTGCCCCA GGACCAGAGG CGCGATGAGG AGAAGATCTA TCATAAGATG
    AACATTGCAG AGCTGCAGGC CCTGGCACCC TCTGTGGACT GGCTGGAGTT TCTGTCTTTC
    TTGTTGTCAC CGCTGGAGCT GGGTGACTCT GAACCTGTGG TGGTGTATGG AACGGATTAT
    TTGCAGCAGG TGTCAGAGCT CATCAACCAC ACAGAGCCAA GTGTCCTGAA CAATTACCTG
    ATTTGGAATC TGGTACAGAA GACAACCTCA AGCCTGGACC ACCGCTTTGA ATCTGCACAG
    GAGAAACTGC TGGAGACTCT CTATGGCACC AAGAAGAGCC GAGAGGGCTG GCCCCAGAGA
    AGAGAAAGGC CCTCAGACTG GAGAACAGAG CTATCTCTGG GAGGCCTGGC TCTTTGTCCC
    TTACAGTCCT GCATGCCAAG GTGGCAGACC TGCATCTCCA ACACGGACGA CGCACTTGGC
    TTCGCTTTGG GCTCCCTCTT TGTGAAGGCC ACGTTCGATC GGCAAAGCAA GGAAATTGCT
    GAAGGGATGA TCAGCGAGAT CCGGAATGCC TTTGAGGAAG CCCTGGGGCA GCTGGTTTGG
    ATGGATGAGA AGACCCGCCA GGCAGCCAAG GAGAAAGCAG ATGCTATATA TGATATGATT
    GGTTTCCCGG ACTTCATCCT GGATCCCAAA GAGCTGGATG ATGTTTATGA TGGGTACGAA
    GTCTCTGAAG ATTCCTTCTT CCAGAACATG TTGAATTTGT ACAATTTCTC TGCTAAGGTG
    ATGGCTGACC AGCTCCGCAA GCCTCCCAGC CGGGACCAGT GGAGCATGAC CCCCCAGACA
    GTGAATGCCT ACTACCTTCC AACCAAGAAT GAAATTGTCT TCCCTGCCGG CATCTTGCAG
    GCCCCCTTCT ACACTCGTAA CCACCCCAAG GCCCTGAACT TTGGTGGAAT CGGCGTGGTG
    ATGGGCCATG AGTTGACACA TGCGTTTGAT GACCAAGGTC GCGAATATGA CAAGGAAGGG
    AACCTGCGGC CCTGGTGGCA GAATGAGTCC CTGGCAGCCT TCCGGAATCA CACAGCTTGC
    ATGGAGGAAC AGTACAGCCA GTACCAGGTC AACGGGGAGA GGCTCAATGG GCGCCAGACG
    CTGGGGGAGA ACATCGCTGA CAACGGGGGG CTGAAGGCTG CCTATAACGC TTACAAAGCA
    TGGCTGAGCA AGCATGGGGA AGAGCAGCAG CTGCCAGCCG TGGGGCTCAC CAATCACCAG
    CTCTTCTTTG TGGGATTTGC CCAGGTGTGG TGCTCAGTCC GCACACCCGA GAGCTCTCAC
    GAGGGGCTGG TGACTGACCC CCACAGCCCC GCCCGCTTCC GCGTGCTGGG CACTCTCTCC
    AACTCCCGGG ACTTCCTGCG ACACTTCGGC TGCCCTGTCG GCTCCCCCAT GAACCCAGGG
    CAGCTGTGTG AGGTGTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44831
    Clone ID Related Accession (Same CDS sequence) XM_005639769.3 , XM_005639769.2
    Accession Version XM_005639769.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2739bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product endothelin-converting enzyme 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005639769.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCCTCTC CGAGGGCCCC GGCCCCGGCG CGGGAGATAC CGGAGCAGAA CTGCTGGTAC 
    CGTGAGGTCC AGTACTGGGA CCGGCGCTAC CGGAATGCCG CGGACTCTGC CCCTTACGAG
    TGGTTCGGAG ACTTCTCCTC CTTCCGTGCC CTCCTGGAGC CTGAGCTGCA GCCAGAAGAC
    CGTATCCTCG TGCTAGGCTG TGGGAACAGC GCGCTGAGCT ACGAGCTGTT CCTTGGGGGC
    TTCCCTGATG TCACCAGTGT GGACTACTCA TCAGTAGTGG TGGCTGCTAT GCAGGCTCGC
    TATGCCCACG TACCCACGCT GCGCTGGGAG ACCATGGATG TACGGGCACT GGACTTCCCC
    AGTGGCTCCT TCGACGTGGT GCTGGAGAAG GGCACGCTGG ATGCCCTGTT AGCTGGAGAA
    CAGGACCCCT GGAATGTGTC TTCTGAAGGT GTCCACACCA TGGATCAGGT GCTGAGTGAG
    ATGGTGGAGT ACGAGCGGAC CACGCTGCGG GAGGAAGACG CACCTGAGAC CCCCGTAGAG
    GGCGGGGCCT CCCCGGACGC TGTGGAGGTG GGATTCCGGA AGAGGACAAG AGACTTCTTG
    GGCTCGCACA CCCAACTGGA GCTGGTCTTA GCCGCTGTCT CTCTACTGCT GGCTGCACTG
    CTACTGGGCT GCTTGGTGGC TCTGGGGGTC CAGTACCACG GGGACCCATC CCACAACACC
    TGCCTCACAG AGGCCTGCAT TCGAGTAGCT GGAAAGATTC TGGAGTCCCT GGACCGCGGG
    GTGAGTCCCT GTGAGGACTT TTACCAGTTT TCCTGTGGTG GCTGGATTCA GAGAAATCCT
    CTGCCCGATG GGCGTTCTCG TTGGAACACC TTCAACAGCC TCTGGGACCA AAATCAGGCC
    ATACTGAAGC ACCTGCTTGA AAACACCACC TTCAACTCTA CCAGTGAAGC TGAGCGGAAG
    ACACAGCGTT TCTACCTCTC CTGCCTGCAG GTGGAGCGGA TCGAGGAACT GGGAGCCCAG
    CCACTGCGAG ACCTCATTGA CAAGATTGGT GGTTGGAACA TTACCGGACC CTGGGACCAG
    GACAACTTCA TGGAAGTGCT GAAGGCAGTA GCAGGGACCT ACAGAGCTAC TCCCTTCTTC
    ACTGTCTACG TCAGTGCCGA CTCTAAGAGC TCTAACAGCA ATGTTATCCA GGTGGACCAA
    TCTGGGCTCT TTCTCCCCTC TCGAGACTAC TACCTAAACA GGACCGCCAA TGAAAAAGTG
    CTTACCGCCT ACCTTGACTA CATGGAGGAG CTGGGGATGT TGCTGGGTGG ACGGCCAACC
    TCCACACGGG AGCAGATGCG GCAGGTGCTG GAGCTGGAGA TACAACTGGC CAACATCACA
    GTGCCCCAGG ACCAGAGGCG CGATGAGGAG AAGATCTATC ATAAGATGAA CATTGCAGAG
    CTGCAGGCCC TGGCACCCTC TGTGGACTGG CTGGAGTTTC TGTCTTTCTT GTTGTCACCG
    CTGGAGCTGG GTGACTCTGA ACCTGTGGTG GTGTATGGAA CGGATTATTT GCAGCAGGTG
    TCAGAGCTCA TCAACCACAC AGAGCCAAGT GTCCTGAACA ATTACCTGAT TTGGAATCTG
    GTACAGAAGA CAACCTCAAG CCTGGACCAC CGCTTTGAAT CTGCACAGGA GAAACTGCTG
    GAGACTCTCT ATGGCACCAA GAAGTCCTGC ATGCCAAGGT GGCAGACCTG CATCTCCAAC
    ACGGACGACG CACTTGGCTT CGCTTTGGGC TCCCTCTTTG TGAAGGCCAC GTTCGATCGG
    CAAAGCAAGG AAATTGCTGA AGGGATGATC AGCGAGATCC GGAATGCCTT TGAGGAAGCC
    CTGGGGCAGC TGGTTTGGAT GGATGAGAAG ACCCGCCAGG CAGCCAAGGA GAAAGCAGAT
    GCTATATATG ATATGATTGG TTTCCCGGAC TTCATCCTGG ATCCCAAAGA GCTGGATGAT
    GTTTATGATG GGTACGAAGT CTCTGAAGAT TCCTTCTTCC AGAACATGTT GAATTTGTAC
    AATTTCTCTG CTAAGGTGAT GGCTGACCAG CTCCGCAAGC CTCCCAGCCG GGACCAGTGG
    AGCATGACCC CCCAGACAGT GAATGCCTAC TACCTTCCAA CCAAGAATGA AATTGTCTTC
    CCTGCCGGCA TCTTGCAGGC CCCCTTCTAC ACTCGTAACC ACCCCAAGGC CCTGAACTTT
    GGTGGAATCG GCGTGGTGAT GGGCCATGAG TTGACACATG CGTTTGATGA CCAAGGTCGC
    GAATATGACA AGGAAGGGAA CCTGCGGCCC TGGTGGCAGA ATGAGTCCCT GGCAGCCTTC
    CGGAATCACA CAGCTTGCAT GGAGGAACAG TACAGCCAGT ACCAGGTCAA CGGGGAGAGG
    CTCAATGGGC GCCAGACGCT GGGGGAGAAC ATCGCTGACA ACGGGGGGCT GAAGGCTGCC
    TATAACGCTT ACAAAGCATG GCTGAGCAAG CATGGGGAAG AGCAGCAGCT GCCAGCCGTG
    GGGCTCACCA ATCACCAGCT CTTCTTTGTG GGATTTGCCC AGGTGTGGTG CTCAGTCCGC
    ACACCCGAGA GCTCTCACGA GGGGCTGGTG ACTGACCCCC ACAGCCCCGC CCGCTTCCGC
    GTGCTGGGCA CTCTCTCCAA CTCCCGGGAC TTCCTGCGAC ACTTCGGCTG CCCTGTCGGC
    TCCCCCATGA ACCCAGGGCA GCTGTGTGAG GTGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005639826.2
    CDS71..2809
    Translation

    Target ORF information:

    RefSeq Version XM_005639769.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris endothelin converting enzyme 2 (ECE2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005639769.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCCTCTC CGAGGGCCCC GGCCCCGGCG CGGGAGATAC CGGAGCAGAA CTGCTGGTAC 
    CGTGAGGTCC AGTACTGGGA CCGGCGCTAC CGGAATGCCG CGGACTCTGC CCCTTACGAG
    TGGTTCGGAG ACTTCTCCTC CTTCCGTGCC CTCCTGGAGC CTGAGCTGCA GCCAGAAGAC
    CGTATCCTCG TGCTAGGCTG TGGGAACAGC GCGCTGAGCT ACGAGCTGTT CCTTGGGGGC
    TTCCCTGATG TCACCAGTGT GGACTACTCA TCAGTAGTGG TGGCTGCTAT GCAGGCTCGC
    TATGCCCACG TACCCACGCT GCGCTGGGAG ACCATGGATG TACGGGCACT GGACTTCCCC
    AGTGGCTCCT TCGACGTGGT GCTGGAGAAG GGCACGCTGG ATGCCCTGTT AGCTGGAGAA
    CAGGACCCCT GGAATGTGTC TTCTGAAGGT GTCCACACCA TGGATCAGGT GCTGAGTGAG
    ATGGTGGAGT ACGAGCGGAC CACGCTGCGG GAGGAAGACG CACCTGAGAC CCCCGTAGAG
    GGCGGGGCCT CCCCGGACGC TGTGGAGGTG GGATTCCGGA AGAGGACAAG AGACTTCTTG
    GGCTCGCACA CCCAACTGGA GCTGGTCTTA GCCGCTGTCT CTCTACTGCT GGCTGCACTG
    CTACTGGGCT GCTTGGTGGC TCTGGGGGTC CAGTACCACG GGGACCCATC CCACAACACC
    TGCCTCACAG AGGCCTGCAT TCGAGTAGCT GGAAAGATTC TGGAGTCCCT GGACCGCGGG
    GTGAGTCCCT GTGAGGACTT TTACCAGTTT TCCTGTGGTG GCTGGATTCA GAGAAATCCT
    CTGCCCGATG GGCGTTCTCG TTGGAACACC TTCAACAGCC TCTGGGACCA AAATCAGGCC
    ATACTGAAGC ACCTGCTTGA AAACACCACC TTCAACTCTA CCAGTGAAGC TGAGCGGAAG
    ACACAGCGTT TCTACCTCTC CTGCCTGCAG GTGGAGCGGA TCGAGGAACT GGGAGCCCAG
    CCACTGCGAG ACCTCATTGA CAAGATTGGT GGTTGGAACA TTACCGGACC CTGGGACCAG
    GACAACTTCA TGGAAGTGCT GAAGGCAGTA GCAGGGACCT ACAGAGCTAC TCCCTTCTTC
    ACTGTCTACG TCAGTGCCGA CTCTAAGAGC TCTAACAGCA ATGTTATCCA GGTGGACCAA
    TCTGGGCTCT TTCTCCCCTC TCGAGACTAC TACCTAAACA GGACCGCCAA TGAAAAAGTG
    CTTACCGCCT ACCTTGACTA CATGGAGGAG CTGGGGATGT TGCTGGGTGG ACGGCCAACC
    TCCACACGGG AGCAGATGCG GCAGGTGCTG GAGCTGGAGA TACAACTGGC CAACATCACA
    GTGCCCCAGG ACCAGAGGCG CGATGAGGAG AAGATCTATC ATAAGATGAA CATTGCAGAG
    CTGCAGGCCC TGGCACCCTC TGTGGACTGG CTGGAGTTTC TGTCTTTCTT GTTGTCACCG
    CTGGAGCTGG GTGACTCTGA ACCTGTGGTG GTGTATGGAA CGGATTATTT GCAGCAGGTG
    TCAGAGCTCA TCAACCACAC AGAGCCAAGT GTCCTGAACA ATTACCTGAT TTGGAATCTG
    GTACAGAAGA CAACCTCAAG CCTGGACCAC CGCTTTGAAT CTGCACAGGA GAAACTGCTG
    GAGACTCTCT ATGGCACCAA GAAGTCCTGC ATGCCAAGGT GGCAGACCTG CATCTCCAAC
    ACGGACGACG CACTTGGCTT CGCTTTGGGC TCCCTCTTTG TGAAGGCCAC GTTCGATCGG
    CAAAGCAAGG AAATTGCTGA AGGGATGATC AGCGAGATCC GGAATGCCTT TGAGGAAGCC
    CTGGGGCAGC TGGTTTGGAT GGATGAGAAG ACCCGCCAGG CAGCCAAGGA GAAAGCAGAT
    GCTATATATG ATATGATTGG TTTCCCGGAC TTCATCCTGG ATCCCAAAGA GCTGGATGAT
    GTTTATGATG GGTACGAAGT CTCTGAAGAT TCCTTCTTCC AGAACATGTT GAATTTGTAC
    AATTTCTCTG CTAAGGTGAT GGCTGACCAG CTCCGCAAGC CTCCCAGCCG GGACCAGTGG
    AGCATGACCC CCCAGACAGT GAATGCCTAC TACCTTCCAA CCAAGAATGA AATTGTCTTC
    CCTGCCGGCA TCTTGCAGGC CCCCTTCTAC ACTCGTAACC ACCCCAAGGC CCTGAACTTT
    GGTGGAATCG GCGTGGTGAT GGGCCATGAG TTGACACATG CGTTTGATGA CCAAGGTCGC
    GAATATGACA AGGAAGGGAA CCTGCGGCCC TGGTGGCAGA ATGAGTCCCT GGCAGCCTTC
    CGGAATCACA CAGCTTGCAT GGAGGAACAG TACAGCCAGT ACCAGGTCAA CGGGGAGAGG
    CTCAATGGGC GCCAGACGCT GGGGGAGAAC ATCGCTGACA ACGGGGGGCT GAAGGCTGCC
    TATAACGCTT ACAAAGCATG GCTGAGCAAG CATGGGGAAG AGCAGCAGCT GCCAGCCGTG
    GGGCTCACCA ATCACCAGCT CTTCTTTGTG GGATTTGCCC AGGTGTGGTG CTCAGTCCGC
    ACACCCGAGA GCTCTCACGA GGGGCTGGTG ACTGACCCCC ACAGCCCCGC CCGCTTCCGC
    GTGCTGGGCA CTCTCTCCAA CTCCCGGGAC TTCCTGCGAC ACTTCGGCTG CCCTGTCGGC
    TCCCCCATGA ACCCAGGGCA GCTGTGTGAG GTGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44831
    Clone ID Related Accession (Same CDS sequence) XM_005639769.3 , XM_005639769.2
    Accession Version XM_005639769.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2739bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product endothelin-converting enzyme 2 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726120.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005639769.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCCTCTC CGAGGGCCCC GGCCCCGGCG CGGGAGATAC CGGAGCAGAA CTGCTGGTAC 
    CGTGAGGTCC AGTACTGGGA CCGGCGCTAC CGGAATGCCG CGGACTCTGC CCCTTACGAG
    TGGTTCGGAG ACTTCTCCTC CTTCCGTGCC CTCCTGGAGC CTGAGCTGCA GCCAGAAGAC
    CGTATCCTCG TGCTAGGCTG TGGGAACAGC GCGCTGAGCT ACGAGCTGTT CCTTGGGGGC
    TTCCCTGATG TCACCAGTGT GGACTACTCA TCAGTAGTGG TGGCTGCTAT GCAGGCTCGC
    TATGCCCACG TACCCACGCT GCGCTGGGAG ACCATGGATG TACGGGCACT GGACTTCCCC
    AGTGGCTCCT TCGACGTGGT GCTGGAGAAG GGCACGCTGG ATGCCCTGTT AGCTGGAGAA
    CAGGACCCCT GGAATGTGTC TTCTGAAGGT GTCCACACCA TGGATCAGGT GCTGAGTGAG
    ATGGTGGAGT ACGAGCGGAC CACGCTGCGG GAGGAAGACG CACCTGAGAC CCCCGTAGAG
    GGCGGGGCCT CCCCGGACGC TGTGGAGGTG GGATTCCGGA AGAGGACAAG AGACTTCTTG
    GGCTCGCACA CCCAACTGGA GCTGGTCTTA GCCGCTGTCT CTCTACTGCT GGCTGCACTG
    CTACTGGGCT GCTTGGTGGC TCTGGGGGTC CAGTACCACG GGGACCCATC CCACAACACC
    TGCCTCACAG AGGCCTGCAT TCGAGTAGCT GGAAAGATTC TGGAGTCCCT GGACCGCGGG
    GTGAGTCCCT GTGAGGACTT TTACCAGTTT TCCTGTGGTG GCTGGATTCA GAGAAATCCT
    CTGCCCGATG GGCGTTCTCG TTGGAACACC TTCAACAGCC TCTGGGACCA AAATCAGGCC
    ATACTGAAGC ACCTGCTTGA AAACACCACC TTCAACTCTA CCAGTGAAGC TGAGCGGAAG
    ACACAGCGTT TCTACCTCTC CTGCCTGCAG GTGGAGCGGA TCGAGGAACT GGGAGCCCAG
    CCACTGCGAG ACCTCATTGA CAAGATTGGT GGTTGGAACA TTACCGGACC CTGGGACCAG
    GACAACTTCA TGGAAGTGCT GAAGGCAGTA GCAGGGACCT ACAGAGCTAC TCCCTTCTTC
    ACTGTCTACG TCAGTGCCGA CTCTAAGAGC TCTAACAGCA ATGTTATCCA GGTGGACCAA
    TCTGGGCTCT TTCTCCCCTC TCGAGACTAC TACCTAAACA GGACCGCCAA TGAAAAAGTG
    CTTACCGCCT ACCTTGACTA CATGGAGGAG CTGGGGATGT TGCTGGGTGG ACGGCCAACC
    TCCACACGGG AGCAGATGCG GCAGGTGCTG GAGCTGGAGA TACAACTGGC CAACATCACA
    GTGCCCCAGG ACCAGAGGCG CGATGAGGAG AAGATCTATC ATAAGATGAA CATTGCAGAG
    CTGCAGGCCC TGGCACCCTC TGTGGACTGG CTGGAGTTTC TGTCTTTCTT GTTGTCACCG
    CTGGAGCTGG GTGACTCTGA ACCTGTGGTG GTGTATGGAA CGGATTATTT GCAGCAGGTG
    TCAGAGCTCA TCAACCACAC AGAGCCAAGT GTCCTGAACA ATTACCTGAT TTGGAATCTG
    GTACAGAAGA CAACCTCAAG CCTGGACCAC CGCTTTGAAT CTGCACAGGA GAAACTGCTG
    GAGACTCTCT ATGGCACCAA GAAGTCCTGC ATGCCAAGGT GGCAGACCTG CATCTCCAAC
    ACGGACGACG CACTTGGCTT CGCTTTGGGC TCCCTCTTTG TGAAGGCCAC GTTCGATCGG
    CAAAGCAAGG AAATTGCTGA AGGGATGATC AGCGAGATCC GGAATGCCTT TGAGGAAGCC
    CTGGGGCAGC TGGTTTGGAT GGATGAGAAG ACCCGCCAGG CAGCCAAGGA GAAAGCAGAT
    GCTATATATG ATATGATTGG TTTCCCGGAC TTCATCCTGG ATCCCAAAGA GCTGGATGAT
    GTTTATGATG GGTACGAAGT CTCTGAAGAT TCCTTCTTCC AGAACATGTT GAATTTGTAC
    AATTTCTCTG CTAAGGTGAT GGCTGACCAG CTCCGCAAGC CTCCCAGCCG GGACCAGTGG
    AGCATGACCC CCCAGACAGT GAATGCCTAC TACCTTCCAA CCAAGAATGA AATTGTCTTC
    CCTGCCGGCA TCTTGCAGGC CCCCTTCTAC ACTCGTAACC ACCCCAAGGC CCTGAACTTT
    GGTGGAATCG GCGTGGTGAT GGGCCATGAG TTGACACATG CGTTTGATGA CCAAGGTCGC
    GAATATGACA AGGAAGGGAA CCTGCGGCCC TGGTGGCAGA ATGAGTCCCT GGCAGCCTTC
    CGGAATCACA CAGCTTGCAT GGAGGAACAG TACAGCCAGT ACCAGGTCAA CGGGGAGAGG
    CTCAATGGGC GCCAGACGCT GGGGGAGAAC ATCGCTGACA ACGGGGGGCT GAAGGCTGCC
    TATAACGCTT ACAAAGCATG GCTGAGCAAG CATGGGGAAG AGCAGCAGCT GCCAGCCGTG
    GGGCTCACCA ATCACCAGCT CTTCTTTGTG GGATTTGCCC AGGTGTGGTG CTCAGTCCGC
    ACACCCGAGA GCTCTCACGA GGGGCTGGTG ACTGACCCCC ACAGCCCCGC CCGCTTCCGC
    GTGCTGGGCA CTCTCTCCAA CTCCCGGGAC TTCCTGCGAC ACTTCGGCTG CCCTGTCGGC
    TCCCCCATGA ACCCAGGGCA GCTGTGTGAG GTGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005639826.2
    CDS62..2800
    Translation

    Target ORF information:

    RefSeq Version XM_005639769.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris endothelin converting enzyme 2 (ECE2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005639769.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCCTCTC CGAGGGCCCC GGCCCCGGCG CGGGAGATAC CGGAGCAGAA CTGCTGGTAC 
    CGTGAGGTCC AGTACTGGGA CCGGCGCTAC CGGAATGCCG CGGACTCTGC CCCTTACGAG
    TGGTTCGGAG ACTTCTCCTC CTTCCGTGCC CTCCTGGAGC CTGAGCTGCA GCCAGAAGAC
    CGTATCCTCG TGCTAGGCTG TGGGAACAGC GCGCTGAGCT ACGAGCTGTT CCTTGGGGGC
    TTCCCTGATG TCACCAGTGT GGACTACTCA TCAGTAGTGG TGGCTGCTAT GCAGGCTCGC
    TATGCCCACG TACCCACGCT GCGCTGGGAG ACCATGGATG TACGGGCACT GGACTTCCCC
    AGTGGCTCCT TCGACGTGGT GCTGGAGAAG GGCACGCTGG ATGCCCTGTT AGCTGGAGAA
    CAGGACCCCT GGAATGTGTC TTCTGAAGGT GTCCACACCA TGGATCAGGT GCTGAGTGAG
    ATGGTGGAGT ACGAGCGGAC CACGCTGCGG GAGGAAGACG CACCTGAGAC CCCCGTAGAG
    GGCGGGGCCT CCCCGGACGC TGTGGAGGTG GGATTCCGGA AGAGGACAAG AGACTTCTTG
    GGCTCGCACA CCCAACTGGA GCTGGTCTTA GCCGCTGTCT CTCTACTGCT GGCTGCACTG
    CTACTGGGCT GCTTGGTGGC TCTGGGGGTC CAGTACCACG GGGACCCATC CCACAACACC
    TGCCTCACAG AGGCCTGCAT TCGAGTAGCT GGAAAGATTC TGGAGTCCCT GGACCGCGGG
    GTGAGTCCCT GTGAGGACTT TTACCAGTTT TCCTGTGGTG GCTGGATTCA GAGAAATCCT
    CTGCCCGATG GGCGTTCTCG TTGGAACACC TTCAACAGCC TCTGGGACCA AAATCAGGCC
    ATACTGAAGC ACCTGCTTGA AAACACCACC TTCAACTCTA CCAGTGAAGC TGAGCGGAAG
    ACACAGCGTT TCTACCTCTC CTGCCTGCAG GTGGAGCGGA TCGAGGAACT GGGAGCCCAG
    CCACTGCGAG ACCTCATTGA CAAGATTGGT GGTTGGAACA TTACCGGACC CTGGGACCAG
    GACAACTTCA TGGAAGTGCT GAAGGCAGTA GCAGGGACCT ACAGAGCTAC TCCCTTCTTC
    ACTGTCTACG TCAGTGCCGA CTCTAAGAGC TCTAACAGCA ATGTTATCCA GGTGGACCAA
    TCTGGGCTCT TTCTCCCCTC TCGAGACTAC TACCTAAACA GGACCGCCAA TGAAAAAGTG
    CTTACCGCCT ACCTTGACTA CATGGAGGAG CTGGGGATGT TGCTGGGTGG ACGGCCAACC
    TCCACACGGG AGCAGATGCG GCAGGTGCTG GAGCTGGAGA TACAACTGGC CAACATCACA
    GTGCCCCAGG ACCAGAGGCG CGATGAGGAG AAGATCTATC ATAAGATGAA CATTGCAGAG
    CTGCAGGCCC TGGCACCCTC TGTGGACTGG CTGGAGTTTC TGTCTTTCTT GTTGTCACCG
    CTGGAGCTGG GTGACTCTGA ACCTGTGGTG GTGTATGGAA CGGATTATTT GCAGCAGGTG
    TCAGAGCTCA TCAACCACAC AGAGCCAAGT GTCCTGAACA ATTACCTGAT TTGGAATCTG
    GTACAGAAGA CAACCTCAAG CCTGGACCAC CGCTTTGAAT CTGCACAGGA GAAACTGCTG
    GAGACTCTCT ATGGCACCAA GAAGTCCTGC ATGCCAAGGT GGCAGACCTG CATCTCCAAC
    ACGGACGACG CACTTGGCTT CGCTTTGGGC TCCCTCTTTG TGAAGGCCAC GTTCGATCGG
    CAAAGCAAGG AAATTGCTGA AGGGATGATC AGCGAGATCC GGAATGCCTT TGAGGAAGCC
    CTGGGGCAGC TGGTTTGGAT GGATGAGAAG ACCCGCCAGG CAGCCAAGGA GAAAGCAGAT
    GCTATATATG ATATGATTGG TTTCCCGGAC TTCATCCTGG ATCCCAAAGA GCTGGATGAT
    GTTTATGATG GGTACGAAGT CTCTGAAGAT TCCTTCTTCC AGAACATGTT GAATTTGTAC
    AATTTCTCTG CTAAGGTGAT GGCTGACCAG CTCCGCAAGC CTCCCAGCCG GGACCAGTGG
    AGCATGACCC CCCAGACAGT GAATGCCTAC TACCTTCCAA CCAAGAATGA AATTGTCTTC
    CCTGCCGGCA TCTTGCAGGC CCCCTTCTAC ACTCGTAACC ACCCCAAGGC CCTGAACTTT
    GGTGGAATCG GCGTGGTGAT GGGCCATGAG TTGACACATG CGTTTGATGA CCAAGGTCGC
    GAATATGACA AGGAAGGGAA CCTGCGGCCC TGGTGGCAGA ATGAGTCCCT GGCAGCCTTC
    CGGAATCACA CAGCTTGCAT GGAGGAACAG TACAGCCAGT ACCAGGTCAA CGGGGAGAGG
    CTCAATGGGC GCCAGACGCT GGGGGAGAAC ATCGCTGACA ACGGGGGGCT GAAGGCTGCC
    TATAACGCTT ACAAAGCATG GCTGAGCAAG CATGGGGAAG AGCAGCAGCT GCCAGCCGTG
    GGGCTCACCA ATCACCAGCT CTTCTTTGTG GGATTTGCCC AGGTGTGGTG CTCAGTCCGC
    ACACCCGAGA GCTCTCACGA GGGGCTGGTG ACTGACCCCC ACAGCCCCGC CCGCTTCCGC
    GTGCTGGGCA CTCTCTCCAA CTCCCGGGAC TTCCTGCGAC ACTTCGGCTG CCCTGTCGGC
    TCCCCCATGA ACCCAGGGCA GCTGTGTGAG GTGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30457
    Clone ID Related Accession (Same CDS sequence) XM_545229.5
    Accession Version XM_545229.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 975bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product endothelin-converting enzyme 2 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726120.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_545229.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGATGTGCT TTTCCCACCC GGAGTATTCT TCCAAGAGCG CTCAGCAGAG GACGGAAGTT 
    CCACGTGTTT CTGTTGAGCA ACTGCAGGTG GTGGGCGGAG GCACGAAGAC CACGCCCCCG
    AGCGCTCCCC TCCCCATTGG CTACACTGGG CGCCAAGCCG GGTCCGGAGG TGCCGGCGCT
    GTGCCGGGCC GCCCAGCAGC CGCGAGCATG GCCTCTCCGA GGGCCCCGGC CCCGGCGCGG
    GAGATACCGG AGCAGAACTG CTGGTACCGT GAGGTCCAGT ACTGGGACCG GCGCTACCGG
    AATGCCGCGG ACTCTGCCCC TTACGAGTGG TTCGGAGACT TCTCCTCCTT CCGTGCCCTC
    CTGGAGCCTG AGCTGCAGCC AGAAGACCGT ATCCTCGTGC TAGGCTGTGG GAACAGCGCG
    CTGAGCTACG AGCTGTTCCT TGGGGGCTTC CCTGATGTCA CCAGTGTGGA CTACTCATCA
    GTAGTGGTGG CTGCTATGCA GGCTCGCTAT GCCCACGTAC CCACGCTGCG CTGGGAGACC
    ATGGATGTAC GGGCACTGGA CTTCCCCAGT GGCTCCTTCG ACGTGGTGCT GGAGAAGGGC
    ACGCTGGATG CCCTGTTAGC TGGAGAACAG GACCCCTGGA ATGTGTCTTC TGAAGGTGTC
    CACACCATGG ATCAGGTGCT GAGTGAGGTG AGCCGGGTGC TGGTCCATGG AGGCCGGTTC
    ATCTCATTGA CCTCAGCTGC TCCCCACTTT CGGATCAGAC ACTATGCCCA AGCCCGATAT
    GGCTGGTCCC TGAGGCATGC AACCTATGGC AGTGGTTTCC ACTTCCATCT TTACATCATG
    CAGAAGGGCA AAGAGCTCAC TGTGGCCCAG CTGGCCCTTG GGACCAAGAT CCTTTCACCC
    CCTAGACCTC CCACCTCGCC CTGCTTCCTC CAGGACTCGG ATCATGAGGA CTTCCTCAGT
    GCCATTCAGC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_545229.2
    CDS115..1089
    Translation

    Target ORF information:

    RefSeq Version XM_545229.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris endothelin converting enzyme 2 (ECE2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_545229.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGATGTGCT TTTCCCACCC GGAGTATTCT TCCAAGAGCG CTCAGCAGAG GACGGAAGTT 
    CCACGTGTTT CTGTTGAGCA ACTGCAGGTG GTGGGCGGAG GCACGAAGAC CACGCCCCCG
    AGCGCTCCCC TCCCCATTGG CTACACTGGG CGCCAAGCCG GGTCCGGAGG TGCCGGCGCT
    GTGCCGGGCC GCCCAGCAGC CGCGAGCATG GCCTCTCCGA GGGCCCCGGC CCCGGCGCGG
    GAGATACCGG AGCAGAACTG CTGGTACCGT GAGGTCCAGT ACTGGGACCG GCGCTACCGG
    AATGCCGCGG ACTCTGCCCC TTACGAGTGG TTCGGAGACT TCTCCTCCTT CCGTGCCCTC
    CTGGAGCCTG AGCTGCAGCC AGAAGACCGT ATCCTCGTGC TAGGCTGTGG GAACAGCGCG
    CTGAGCTACG AGCTGTTCCT TGGGGGCTTC CCTGATGTCA CCAGTGTGGA CTACTCATCA
    GTAGTGGTGG CTGCTATGCA GGCTCGCTAT GCCCACGTAC CCACGCTGCG CTGGGAGACC
    ATGGATGTAC GGGCACTGGA CTTCCCCAGT GGCTCCTTCG ACGTGGTGCT GGAGAAGGGC
    ACGCTGGATG CCCTGTTAGC TGGAGAACAG GACCCCTGGA ATGTGTCTTC TGAAGGTGTC
    CACACCATGG ATCAGGTGCT GAGTGAGGTG AGCCGGGTGC TGGTCCATGG AGGCCGGTTC
    ATCTCATTGA CCTCAGCTGC TCCCCACTTT CGGATCAGAC ACTATGCCCA AGCCCGATAT
    GGCTGGTCCC TGAGGCATGC AACCTATGGC AGTGGTTTCC ACTTCCATCT TTACATCATG
    CAGAAGGGCA AAGAGCTCAC TGTGGCCCAG CTGGCCCTTG GGACCAAGAT CCTTTCACCC
    CCTAGACCTC CCACCTCGCC CTGCTTCCTC CAGGACTCGG ATCATGAGGA CTTCCTCAGT
    GCCATTCAGC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.