PLCE1 cDNA ORF clone, Canis lupus familiaris(Dog)

The following PLCE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLCE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe43815 XM_014108763.1
Latest version!
Canis lupus familiaris phospholipase C, epsilon 1 (PLCE1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe43814 XM_014108762.1
Latest version!
Canis lupus familiaris phospholipase C, epsilon 1 (PLCE1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OCe43814 XM_022411586.1
Latest version!
Canis lupus familiaris phospholipase C epsilon 1 (PLCE1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OCe27696 NM_001136565.1
Latest version!
Canis lupus familiaris phospholipase C epsilon 1 (PLCE1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe27695 XM_005637458.2
Latest version!
Canis lupus familiaris phospholipase C, epsilon 1 (PLCE1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe27695 XM_022411584.1
Latest version!
Canis lupus familiaris phospholipase C epsilon 1 (PLCE1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe27695 XM_022411585.1
Latest version!
Canis lupus familiaris phospholipase C epsilon 1 (PLCE1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe27696 XM_022411587.1
Latest version!
Canis lupus familiaris phospholipase C epsilon 1 (PLCE1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe27696 XM_005637459.2
Latest version!
Canis lupus familiaris phospholipase C, epsilon 1 (PLCE1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe27697 XM_022411588.1
Latest version!
Canis lupus familiaris phospholipase C epsilon 1 (PLCE1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe27697 XM_005637460.2
Latest version!
Canis lupus familiaris phospholipase C, epsilon 1 (PLCE1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe27698 XM_005637461.2
Latest version!
Canis lupus familiaris phospholipase C, epsilon 1 (PLCE1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe27698 XM_022411589.1
Latest version!
Canis lupus familiaris phospholipase C epsilon 1 (PLCE1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe43815
    Clone ID Related Accession (Same CDS sequence) XM_014108763.1
    Accession Version XM_014108763.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5751bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGAAGAGA TCTACAATGC AGTGAGAAGG GAAGAAGAAA TAGAAAATAC CATTGGATCT 
    CTTCTCCATT ACTTTACCAA GCTCCCAGCC TCCAAGACAG CCCATGAAAG GATTAGTGTT
    GGTCCATGCC TAAAGCAGTG TGTCCGAGAC ACCATATGTG AGTATCGTGC CACCCTCCAG
    AGGACGTCCA TATCTCAGTA CATCACGGGT TCGCTCCTAG AAGCAACCAC ATCTTTAGGA
    GCGAGAAGTG GCCTACTCAG TACTTTTGGA GGATCCACTG GACGAATGAT GCTAAAAGAA
    CGCCAGCCAG GCACCTCCAT GGCCAATTCC AGTGCCCTCC CTTCAAGTTC CGCTGGAATC
    AGCAAGGAGC TGATTGACCT GCAGCCTCTC ATTCAGTTCC CAGAGGAAGT CGCCAGCATC
    CTGATGGAGC AGGAACAGAA CATTTACCGA AGGGTCTTAC CAGTTGACTA TCTTTACTTC
    TTGACCCGGG ACTTGGGCAC TCCAGAATGC CAGACACCCC TGCCCTGCCT CAAAGCATCC
    ATCTCAGCTT CAATTCTTAG CTCCCAGAAC GGAGAGCACA ATGCCCTGGA AGATCTTGTG
    ATGAGATTTA ATGAGGTGAG CTCCTGGGTG ACATGGCTGA TCCTCACGGC GGGCTCCATG
    GAGGAGAAGC GAGAAGTCTT CTCCTATTTG GTGCATGTGG CCAAATGCTG CTGGAACATG
    GGCAACTACA ATGCTGTCAT GGAGTTCTTG GCTGGTCTCA GGTCAAGAAA AGTTTTAAAA
    ATGTGGCAGT TTATGGACCA ATCTGACCTC GAGACCATGA GGAGCCTGAA GGACGCCATG
    GCTCAGCATG AATCTTCCTG CGAATATCGG AAGGTGATAA CGAGGGCTCT GCACATACCT
    GGCTGTAAAG TGGTTCCATT CTGTGGAGTG TTTCTGAAGG AGCTCTGTGA AGTACTGGAC
    GGAGCCTCTG GCCTCATGAA GCTTTGCCCG AGGTACAATT CCCAAGAAGA AACTCTAGAG
    TTTGTAGCAG ATTACAGTGG ACAAGATAAT TTCTTACAAC GAGTGGGGCA TAATGGCTTA
    AAGAATTCAG AGAAGGAGTC CACTGTCAAC AGCATCTTTC AAATCATTAG GAGCTGCAGC
    CGAAGTCTGG AGGCTGAGGA AGAGGACAGC CCCAGTGAAG GGAACAGCTC TAGGAAAAAT
    TCTTTAAGGG ACAAAGCCCG ATGGCAATTT ATAATCGGAG ATTTGTTGGA TTCCGAAAAT
    GACATCTTTG AGCAATCCAA AGAATGGGAC TCTCCCAGCT CAGAAGAGCC TCAGAAAGCC
    TTTGACCATG GGACAGAGCT GATCCCTTGG TACGTGCTGT CCATCCGAGC AGATGTGCAC
    CAGTTCCTGC TACAGGGCGC CACCGTCCTC CGCTACGACC AGGACACGCA CCTCTCTGCC
    CGCTGCTTCC TCCAGCTTCA GCCTGACAAT AGCACCTTGA CATGGATAAA GCCCACAGCT
    GCCTCCCCGG CCAGTGCTAG AGCAAAACTC GGTGTGCTGA GCAACACGTC TGAGCCTGGC
    AAGTTCCCGT CACCAGGCAG TGCCGGACTA AGCGGCCTGG CCGAGGGGGT CCTGGATCTG
    TTTTCAGCAA AGGCTGTGTA CATGGGACAC CCTAGCATTG ATATCCACAC TGTGTGTGTC
    CAGAACAAAC TCGGTAGCAT GTTTCTCTCA GAGACTGGCG TGACCCTGCT CTACGGGCTC
    CAGACCACAG ACAATAGATT ATTGCACTTT GTGGCACCCA AACACACTGC TAAGATGCTC
    TTCAGTGGAT TGCTAGAACT CACGAGGGCC GTGAGGAAGA TGAGAAAGTT CCCTGACCAG
    AGGCAGCAGT GGCTGCGGAA ACAGTATGTT AGCCTCTATC AGGAGGACGG ACGGTATGAG
    GGCCCAACTT TGGCTCATGC TGTGGAGTTG TTTGGTGGCC GGCGATGGAG TACACGCAAT
    CCCAGCCCTG GGACATCTGC AAAGAGTGCT GAGAAGCCCA GTGTGCAGAG AAACAACACC
    TTGGGCATAA GCACCACCAA GAAAAAGAAG AAAATCCTTA TAAGGGGTGA GAGTGGAGAG
    GCAGCCGACG ATGAGATGGC CACCCGGAAG GCCAAAATGC ACAGGGAGTG TCGCAGCCGG
    AGTGGTTCTG ACCCACAAGA CATGAATGAG CAAGAAGAAT CTGAGGCAAA TGCCATCATG
    AGCCCTCCTA ACACTCTGCC TTCCAGAAGA GCCCACTCTT TGACTACAGC TGGGTCCCCT
    AATTTGTCTG CCGGGACATC ATCTCCCATC AGGCCAGTGT CCTCCCCTGT GCTGTCTTCT
    TCAAACAAGA GCCCGTCCAG TGCTTGGAGC AGCAGCAGCT GGCATGGAAG GATCAAAGGA
    GGAATGAAGG GTTTTCAGAG CTTCATGGTT TCAGACAGTA ACATGAGTTT TGTTGAATTT
    GTTGAGCTGT TCAAATCATT CAGTGTGAGG AGCCGCAAGG ACCTGAAGGA TCTCTTTGAC
    ATCTACGCTG TGCCCTGCAA TCGGGCCGGC TCTGAGTCGG CCCCGCTCTA CACCAACCTA
    ACAATAGACG AAAACACCAG CGATCTTCAG CCTGACCTAG ATTTATTGAC CAGAAACGTC
    TCGGATTTGG GGTTGTTCAT TAAGAGTAAA CAGCAGCTGT CAGACAACCA GAGGCAGATT
    TCTGATGCCA TTGCTGCTGC AAGTATCGTG ACGAATGGCA CTGGGATCGA GAGCACATCT
    TTGGGCATCT TTGGGGTTGG CATACTCCAG CTCAATGACT TCCTAGTCAA CTGCCAGGGA
    GAACACTGCA CTTACGATGA AATCCTCAGC ATCATCCAGA AGTTCGAGCC TAGCATTAGT
    ATGTGTCATC AGGGCCTACT GTCCTTTGAA GGATTTGCAA GGTTTCTGAT GGATAAAGAT
    AATTTTGCCT CCAAAAATGA TGAGTCACAG GAGAACATTA AAGATTTGCA GCTGCCTCTC
    TCCTACTATT ACATTGAGTC TTCACACAAT ACCTACCTCA CAGGCCACCA GCTCAAAGGA
    GAGTCATCGG TAGAACTCTA TAGCCAGGTC CTCCTGCAAG GCTGTAGAAG TGTAGAGTTG
    GACTGCTGGG ATGGAGATGA CGGGATGCCC ATCATCTATC ACGGACACAC ACTAACAACC
    AAGATCCCCT TCAAGGAGGT GGTTGAAGCC ATCGACCGCA GTGCCTTCAT CAACTCTGAC
    CTGCCCATCA TCATATCGAT TGAGAACCAC TGCTCGTTGC CTCAGCAACG AAAAATGGCA
    GAAATTTTCA AGACTGTGTT TGGAGAAAAA CTGGTGGCTA AATTCCTGTT TGAGAGCGAT
    TTCTCAGATG ATCCAATGCT TCCCTCACCT GACCAACTTA GGAGGAAAGT GCTTCTTAAA
    AATAAGAAGC TAAAAGCCCA CCAGACACCA GTGGATATCT TAAAGCAAAA GGCTCATCAG
    TTAGCATCCA TGCAAGCTCA GGCTTATAAT GGGGGAAATG TCAACCCTCC ACCTGCTAAT
    AACGAGGAAG AGGAAGATGA AGAGGACGAA TATGATTATG ACTATGAATC CCTGTCTGAT
    GCAGATGTCC TTACTGCTTC TCCTGCTCCT AACAGTCAGG AAGACAACAT TCTAGAAGAC
    AGACCTGAAA ATAAATCATG TAATGACAAG CTTCAGTTTG AGTATAACGA AGAAATCCCC
    AAGAGGATAA AGAAAGCAGA TAATTCTGCT TTCAACAAAG GAAAGGTTTA TGACATGGAA
    CTGGGAGAAG AATTTTATCT TCCTCAGAAT AAAAAGGAAA GCAGACAGAT TGCACCAGAG
    CTTTCTGACC TTGTCATCTA CTGCCAAGCG GTAAAGTTTC CGGGGCTGTC AACTCTAAAT
    GCATCTGGCT CTAACAGAGG AAAAGAGAGG AAAAGCAGGA AGTCCATTTT TGGCAACAAT
    CCGGGCAGAA TGAGCCCTGG GGAGACAGCA TCGTTTAACA AAGCATCTGG AAAAAGTTCC
    TGTGAAGGAA TGCGACAGGC GTGGGAGGAA TCTTCTTCCC CCCTCAACCC AACCACATCC
    CTCAGCGCTA TCATTAGAAC TCCCAAATGT TACCATATCT CATCGCTGAA TGAAAATGCC
    GCCAAACGTC TGTGTCGCAG GTATTCTCAG AAACTGATCC AGCACACTGC CTGTCAGCTG
    CTGAGGACTT ACCCGGCTGC CACACGCATC GACTCCTCCA ACCCCAATCC TCTCTTGTTC
    TGGCTCCATG GGATACAGCT CGTGGCACTC AACTACCAGA CAGATGATCT CCCTTTACAT
    TTAAATGCTG CCATGTTTGA GGCCAACGGT GGCTGTGGTT ATGTTTTGAA ACCCCCCGTT
    CTGTGGGACA AGAACTGTCC CATGTACCAG AAGTTTTCAC CCTTGGAAAG AGACCTGGAC
    AACATGGAGC CTGCCGTCTA TTCCTTAACT ATTGTCTCTG GTCAAAACGT GTGCCCAAGT
    AGCAGCACGG GAAGCCCATG TATCGAAGTG GACGTGCTGG GCATGCCCCT GGACAGTTGC
    CACTTCCGCA CAAAACCCAT CCACCGGAAC ACTTTGAACC CCATGTGGAA TGAACAGTTT
    CTCTTCCGGG TTCACTTTGA AGATCTCATA TTCCTGCGTT TTGCAGTTGT GGAAAACAAT
    AGCTCAGCCA TAACCGCTCA GAGAATCATC CCCCTCAAGG CTTTAAAACG AGGATATCGA
    CATCTTCAGC TGCGAAACCT CCACAATGAA GTCTTGGAAA TCTCTAGTTT ATTCATAAAC
    AGCAGAAGGA TGGAAGAAAA TTCCTCCAGC AGTGCCACGC CAGCCTCTTT GATGTTTAAT
    ACAGAAGAAA GAAAATGTTT GCAGACTCAC AGAGTCACAG TGCATGGGGT CCCAGGTCCA
    GAGCCCTTTA CTGTTTTCTC CATAAGTGGA GGCACGAAGG CAAAGCAGCT TCTCCAACAG
    ATTCTAACTA CTGAACAAGA CACCAAACCT ATTGTCACAG ACTATTTTTT GATGGAAGAA
    AAATATTTTA TATCTAAAGA AAAGAATGAA TGCAGGAAAC AACCATTTCA GAGAGTCATC
    GGTCCAGAAG AAGAGATCAT GCAGATTTTA AGCAGCTGGT TTCCAGAGGA AGGATATGTT
    GGCAGGATTG TCTTAAAAAC CCAGCAGGAA AACCTAGAAG AGAGAAGCAT TGTTCAAGAT
    GAGAAAGAGG TGATCTTGAG CTCGGAGGAG GAGAGCTTCT TTGTCCAAGT GCATGACGTT
    TCTCCAGAGC AACCTCGGAC GGTCATCAAA GCGCCCCGTG TCAGCACCGC TCAGGATGTC
    ATTCAACAGA CCTTATGCAA AGCCAAATAT TCCTATAGCA TCCTGAGTAA CCCCAACCCG
    AGTGACTATG TGCTTCTGGA AGAGGTGGTG AAAGATGCTA CCAAGAAGTC CTCCACCCCA
    AAGTCCTCCC AGCGGGTCCT TTTGGACCAA GAGTGTGTGT TTCAAGCCCA GAGCAAGTGG
    AAAGGTGCTG GAAAATTCAT CCTCAGGCTA AAGGAGCAGG TGCAGGCATC CCGAGAAGAT
    AAAAGAAAAG GCATTTCTTT CGCAAGTGAA TTCAAGAAGT TCACCAAATC AACTAAACAG
    CCCCGAGGAC TCACATCTTC TCAGGTCTTG GCCTCAGAAA GTGTCCAAAA CAAGGAGGAG
    AAGCCCACGG GCAGCTTGTC CTCCAGTGAC ACCACAGACT CCCGACAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013964238.1
    CDS26..5776
    Translation

    Target ORF information:

    RefSeq Version XM_014108763.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris phospholipase C, epsilon 1 (PLCE1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108763.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGAAGAGA TCTACAATGC AGTGAGAAGG GAAGAAGAAA TAGAAAATAC CATTGGATCT 
    CTTCTCCATT ACTTTACCAA GCTCCCAGCC TCCAAGACAG CCCATGAAAG GATTAGTGTT
    GGTCCATGCC TAAAGCAGTG TGTCCGAGAC ACCATATGTG AGTATCGTGC CACCCTCCAG
    AGGACGTCCA TATCTCAGTA CATCACGGGT TCGCTCCTAG AAGCAACCAC ATCTTTAGGA
    GCGAGAAGTG GCCTACTCAG TACTTTTGGA GGATCCACTG GACGAATGAT GCTAAAAGAA
    CGCCAGCCAG GCACCTCCAT GGCCAATTCC AGTGCCCTCC CTTCAAGTTC CGCTGGAATC
    AGCAAGGAGC TGATTGACCT GCAGCCTCTC ATTCAGTTCC CAGAGGAAGT CGCCAGCATC
    CTGATGGAGC AGGAACAGAA CATTTACCGA AGGGTCTTAC CAGTTGACTA TCTTTACTTC
    TTGACCCGGG ACTTGGGCAC TCCAGAATGC CAGACACCCC TGCCCTGCCT CAAAGCATCC
    ATCTCAGCTT CAATTCTTAG CTCCCAGAAC GGAGAGCACA ATGCCCTGGA AGATCTTGTG
    ATGAGATTTA ATGAGGTGAG CTCCTGGGTG ACATGGCTGA TCCTCACGGC GGGCTCCATG
    GAGGAGAAGC GAGAAGTCTT CTCCTATTTG GTGCATGTGG CCAAATGCTG CTGGAACATG
    GGCAACTACA ATGCTGTCAT GGAGTTCTTG GCTGGTCTCA GGTCAAGAAA AGTTTTAAAA
    ATGTGGCAGT TTATGGACCA ATCTGACCTC GAGACCATGA GGAGCCTGAA GGACGCCATG
    GCTCAGCATG AATCTTCCTG CGAATATCGG AAGGTGATAA CGAGGGCTCT GCACATACCT
    GGCTGTAAAG TGGTTCCATT CTGTGGAGTG TTTCTGAAGG AGCTCTGTGA AGTACTGGAC
    GGAGCCTCTG GCCTCATGAA GCTTTGCCCG AGGTACAATT CCCAAGAAGA AACTCTAGAG
    TTTGTAGCAG ATTACAGTGG ACAAGATAAT TTCTTACAAC GAGTGGGGCA TAATGGCTTA
    AAGAATTCAG AGAAGGAGTC CACTGTCAAC AGCATCTTTC AAATCATTAG GAGCTGCAGC
    CGAAGTCTGG AGGCTGAGGA AGAGGACAGC CCCAGTGAAG GGAACAGCTC TAGGAAAAAT
    TCTTTAAGGG ACAAAGCCCG ATGGCAATTT ATAATCGGAG ATTTGTTGGA TTCCGAAAAT
    GACATCTTTG AGCAATCCAA AGAATGGGAC TCTCCCAGCT CAGAAGAGCC TCAGAAAGCC
    TTTGACCATG GGACAGAGCT GATCCCTTGG TACGTGCTGT CCATCCGAGC AGATGTGCAC
    CAGTTCCTGC TACAGGGCGC CACCGTCCTC CGCTACGACC AGGACACGCA CCTCTCTGCC
    CGCTGCTTCC TCCAGCTTCA GCCTGACAAT AGCACCTTGA CATGGATAAA GCCCACAGCT
    GCCTCCCCGG CCAGTGCTAG AGCAAAACTC GGTGTGCTGA GCAACACGTC TGAGCCTGGC
    AAGTTCCCGT CACCAGGCAG TGCCGGACTA AGCGGCCTGG CCGAGGGGGT CCTGGATCTG
    TTTTCAGCAA AGGCTGTGTA CATGGGACAC CCTAGCATTG ATATCCACAC TGTGTGTGTC
    CAGAACAAAC TCGGTAGCAT GTTTCTCTCA GAGACTGGCG TGACCCTGCT CTACGGGCTC
    CAGACCACAG ACAATAGATT ATTGCACTTT GTGGCACCCA AACACACTGC TAAGATGCTC
    TTCAGTGGAT TGCTAGAACT CACGAGGGCC GTGAGGAAGA TGAGAAAGTT CCCTGACCAG
    AGGCAGCAGT GGCTGCGGAA ACAGTATGTT AGCCTCTATC AGGAGGACGG ACGGTATGAG
    GGCCCAACTT TGGCTCATGC TGTGGAGTTG TTTGGTGGCC GGCGATGGAG TACACGCAAT
    CCCAGCCCTG GGACATCTGC AAAGAGTGCT GAGAAGCCCA GTGTGCAGAG AAACAACACC
    TTGGGCATAA GCACCACCAA GAAAAAGAAG AAAATCCTTA TAAGGGGTGA GAGTGGAGAG
    GCAGCCGACG ATGAGATGGC CACCCGGAAG GCCAAAATGC ACAGGGAGTG TCGCAGCCGG
    AGTGGTTCTG ACCCACAAGA CATGAATGAG CAAGAAGAAT CTGAGGCAAA TGCCATCATG
    AGCCCTCCTA ACACTCTGCC TTCCAGAAGA GCCCACTCTT TGACTACAGC TGGGTCCCCT
    AATTTGTCTG CCGGGACATC ATCTCCCATC AGGCCAGTGT CCTCCCCTGT GCTGTCTTCT
    TCAAACAAGA GCCCGTCCAG TGCTTGGAGC AGCAGCAGCT GGCATGGAAG GATCAAAGGA
    GGAATGAAGG GTTTTCAGAG CTTCATGGTT TCAGACAGTA ACATGAGTTT TGTTGAATTT
    GTTGAGCTGT TCAAATCATT CAGTGTGAGG AGCCGCAAGG ACCTGAAGGA TCTCTTTGAC
    ATCTACGCTG TGCCCTGCAA TCGGGCCGGC TCTGAGTCGG CCCCGCTCTA CACCAACCTA
    ACAATAGACG AAAACACCAG CGATCTTCAG CCTGACCTAG ATTTATTGAC CAGAAACGTC
    TCGGATTTGG GGTTGTTCAT TAAGAGTAAA CAGCAGCTGT CAGACAACCA GAGGCAGATT
    TCTGATGCCA TTGCTGCTGC AAGTATCGTG ACGAATGGCA CTGGGATCGA GAGCACATCT
    TTGGGCATCT TTGGGGTTGG CATACTCCAG CTCAATGACT TCCTAGTCAA CTGCCAGGGA
    GAACACTGCA CTTACGATGA AATCCTCAGC ATCATCCAGA AGTTCGAGCC TAGCATTAGT
    ATGTGTCATC AGGGCCTACT GTCCTTTGAA GGATTTGCAA GGTTTCTGAT GGATAAAGAT
    AATTTTGCCT CCAAAAATGA TGAGTCACAG GAGAACATTA AAGATTTGCA GCTGCCTCTC
    TCCTACTATT ACATTGAGTC TTCACACAAT ACCTACCTCA CAGGCCACCA GCTCAAAGGA
    GAGTCATCGG TAGAACTCTA TAGCCAGGTC CTCCTGCAAG GCTGTAGAAG TGTAGAGTTG
    GACTGCTGGG ATGGAGATGA CGGGATGCCC ATCATCTATC ACGGACACAC ACTAACAACC
    AAGATCCCCT TCAAGGAGGT GGTTGAAGCC ATCGACCGCA GTGCCTTCAT CAACTCTGAC
    CTGCCCATCA TCATATCGAT TGAGAACCAC TGCTCGTTGC CTCAGCAACG AAAAATGGCA
    GAAATTTTCA AGACTGTGTT TGGAGAAAAA CTGGTGGCTA AATTCCTGTT TGAGAGCGAT
    TTCTCAGATG ATCCAATGCT TCCCTCACCT GACCAACTTA GGAGGAAAGT GCTTCTTAAA
    AATAAGAAGC TAAAAGCCCA CCAGACACCA GTGGATATCT TAAAGCAAAA GGCTCATCAG
    TTAGCATCCA TGCAAGCTCA GGCTTATAAT GGGGGAAATG TCAACCCTCC ACCTGCTAAT
    AACGAGGAAG AGGAAGATGA AGAGGACGAA TATGATTATG ACTATGAATC CCTGTCTGAT
    GCAGATGTCC TTACTGCTTC TCCTGCTCCT AACAGTCAGG AAGACAACAT TCTAGAAGAC
    AGACCTGAAA ATAAATCATG TAATGACAAG CTTCAGTTTG AGTATAACGA AGAAATCCCC
    AAGAGGATAA AGAAAGCAGA TAATTCTGCT TTCAACAAAG GAAAGGTTTA TGACATGGAA
    CTGGGAGAAG AATTTTATCT TCCTCAGAAT AAAAAGGAAA GCAGACAGAT TGCACCAGAG
    CTTTCTGACC TTGTCATCTA CTGCCAAGCG GTAAAGTTTC CGGGGCTGTC AACTCTAAAT
    GCATCTGGCT CTAACAGAGG AAAAGAGAGG AAAAGCAGGA AGTCCATTTT TGGCAACAAT
    CCGGGCAGAA TGAGCCCTGG GGAGACAGCA TCGTTTAACA AAGCATCTGG AAAAAGTTCC
    TGTGAAGGAA TGCGACAGGC GTGGGAGGAA TCTTCTTCCC CCCTCAACCC AACCACATCC
    CTCAGCGCTA TCATTAGAAC TCCCAAATGT TACCATATCT CATCGCTGAA TGAAAATGCC
    GCCAAACGTC TGTGTCGCAG GTATTCTCAG AAACTGATCC AGCACACTGC CTGTCAGCTG
    CTGAGGACTT ACCCGGCTGC CACACGCATC GACTCCTCCA ACCCCAATCC TCTCTTGTTC
    TGGCTCCATG GGATACAGCT CGTGGCACTC AACTACCAGA CAGATGATCT CCCTTTACAT
    TTAAATGCTG CCATGTTTGA GGCCAACGGT GGCTGTGGTT ATGTTTTGAA ACCCCCCGTT
    CTGTGGGACA AGAACTGTCC CATGTACCAG AAGTTTTCAC CCTTGGAAAG AGACCTGGAC
    AACATGGAGC CTGCCGTCTA TTCCTTAACT ATTGTCTCTG GTCAAAACGT GTGCCCAAGT
    AGCAGCACGG GAAGCCCATG TATCGAAGTG GACGTGCTGG GCATGCCCCT GGACAGTTGC
    CACTTCCGCA CAAAACCCAT CCACCGGAAC ACTTTGAACC CCATGTGGAA TGAACAGTTT
    CTCTTCCGGG TTCACTTTGA AGATCTCATA TTCCTGCGTT TTGCAGTTGT GGAAAACAAT
    AGCTCAGCCA TAACCGCTCA GAGAATCATC CCCCTCAAGG CTTTAAAACG AGGATATCGA
    CATCTTCAGC TGCGAAACCT CCACAATGAA GTCTTGGAAA TCTCTAGTTT ATTCATAAAC
    AGCAGAAGGA TGGAAGAAAA TTCCTCCAGC AGTGCCACGC CAGCCTCTTT GATGTTTAAT
    ACAGAAGAAA GAAAATGTTT GCAGACTCAC AGAGTCACAG TGCATGGGGT CCCAGGTCCA
    GAGCCCTTTA CTGTTTTCTC CATAAGTGGA GGCACGAAGG CAAAGCAGCT TCTCCAACAG
    ATTCTAACTA CTGAACAAGA CACCAAACCT ATTGTCACAG ACTATTTTTT GATGGAAGAA
    AAATATTTTA TATCTAAAGA AAAGAATGAA TGCAGGAAAC AACCATTTCA GAGAGTCATC
    GGTCCAGAAG AAGAGATCAT GCAGATTTTA AGCAGCTGGT TTCCAGAGGA AGGATATGTT
    GGCAGGATTG TCTTAAAAAC CCAGCAGGAA AACCTAGAAG AGAGAAGCAT TGTTCAAGAT
    GAGAAAGAGG TGATCTTGAG CTCGGAGGAG GAGAGCTTCT TTGTCCAAGT GCATGACGTT
    TCTCCAGAGC AACCTCGGAC GGTCATCAAA GCGCCCCGTG TCAGCACCGC TCAGGATGTC
    ATTCAACAGA CCTTATGCAA AGCCAAATAT TCCTATAGCA TCCTGAGTAA CCCCAACCCG
    AGTGACTATG TGCTTCTGGA AGAGGTGGTG AAAGATGCTA CCAAGAAGTC CTCCACCCCA
    AAGTCCTCCC AGCGGGTCCT TTTGGACCAA GAGTGTGTGT TTCAAGCCCA GAGCAAGTGG
    AAAGGTGCTG GAAAATTCAT CCTCAGGCTA AAGGAGCAGG TGCAGGCATC CCGAGAAGAT
    AAAAGAAAAG GCATTTCTTT CGCAAGTGAA TTCAAGAAGT TCACCAAATC AACTAAACAG
    CCCCGAGGAC TCACATCTTC TCAGGTCTTG GCCTCAGAAA GTGTCCAAAA CAAGGAGGAG
    AAGCCCACGG GCAGCTTGTC CTCCAGTGAC ACCACAGACT CCCGACAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe43814
    Clone ID Related Accession (Same CDS sequence) XM_014108762.1 , XM_022411586.1
    Accession Version XM_014108762.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6969bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGACTTCTG AAGGAATGGC AGCTTCTGTT CTCACACCTG TGACTCAAAG AAAAGTGACA 
    TTTGCTCCAT CGGCTGTAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAG TGTTCCAAAG
    GCACATAGTG TCAAGCAGAG TGAGCAGACT TCTACCATCC CGTGGATGAA CAAGCTTAAA
    GAAGAATCTT CTGGAAGCAA CTTGCCCAAA ATTCTCTCCA TAGCAAGGGA GAAAATAGCA
    AGTGATGAGA ACAGCAATGA AGAGTGCTGG ACGGAAAGCA CACCAGTTTC TGTGAAAAAC
    CTTAACATTA ACCATAACAA CATCCTGACA AACCGCCAGT GCGTCCTTCC TCAGAGCCAG
    TCTTATGACA CGTGCAATTC TGTCATGGAG GAAGACCCAT GTTTAGAAAC TGGAATCTCA
    TCCTCACTGG AAAGAAAGGT ATTCCCTGGA ATTCAACTGG AAGTCAACAG ACCTCCCATG
    GACTTTAGGC CTCCAGGCCT AATGGACTTT AGTACCTTAG GAAGTCAGTC AGCCATTGTA
    GATACTGGCC AGGCGCACCC TGACAGCAAC AAAGCAGCAT TTCAAATCTT TAATTATAAA
    GTTGACAGAA GGATGTCTGA CACTTTCTGT ACCCTGTCAG GTGACTTGAT ATTGGATGAC
    TGTGGAAACT GTGTGCCACT GTCATCTGGT TTTGGCGGGG AGCAAAAGAA AAATTACGTG
    GCATATACCT GTAAACTCAT GGAATTGGCT GAAAACTGTG ATAATGAGAA TGGACAGCTG
    CAGTGTGATG ATTATGATGC CTTGGATGAC AAATACTTGT GCTTTGAAGA TTCTTGCCAA
    AGGGACAGTG TGGTATGTTC AAGTGACAGC TTTCGCAGGG AGGACCTAAC TAACAGTCCA
    CCTGCCAAGA CCTTTCTGAG CCATTTTGAA GACTTCCCTG ATAATGGTGA AGATGTAGAA
    GATTTTTTAA AAAACAAAAA GGAGAGATCC ACTTTGTTAG TGAGGAGATT TTGTAAAAAT
    GACAGAGAAG TAAAGAAATC TGTGTATACT GGGACAAGGG CAATCATGAG AACTCTGCCT
    TCTGGTCACA TTGGGCTGGA AGCTTACAGT TACATCGATC AGAAGAGAAG TGGTCCCTTA
    TTACCCCGTG GGAGAGTTCT GGAACAGCTG CCAGTGGTGG CAATTAGGCA AGATGGGAGC
    CAGTGCCTGT CAGAAGCCCA GTGGTATCGG ATCTACAATG CAGTGAGAAG GGAAGAAGAA
    ATAGAAAATA CCATTGGATC TCTTCTCCAT TACTTTACCA AGCTCCCAGC CTCCAAGACA
    GCCCATGAAA GGATTAGTGT TGGTCCATGC CTAAAGCAGT GTGTCCGAGA CACCATATGT
    GAGTATCGTG CCACCCTCCA GAGGACGTCC ATATCTCAGT ACATCACGGG TTCGCTCCTA
    GAAGCAACCA CATCTTTAGG AGCGAGAAGT GGCCTACTCA GTACTTTTGG AGGATCCACT
    GGACGAATGA TGCTAAAAGA ACGCCAGCCA GGCACCTCCA TGGCCAATTC CAGTGCCCTC
    CCTTCAAGTT CCGCTGGAAT CAGCAAGGAG CTGATTGACC TGCAGCCTCT CATTCAGTTC
    CCAGAGGAAG TCGCCAGCAT CCTGATGGAG CAGGAACAGA ACATTTACCG AAGGGTCTTA
    CCAGTTGACT ATCTTTACTT CTTGACCCGG GACTTGGGCA CTCCAGAATG CCAGACACCC
    CTGCCCTGCC TCAAAGCATC CATCTCAGCT TCAATTCTTA GCTCCCAGAA CGGAGAGCAC
    AATGCCCTGG AAGATCTTGT GATGAGATTT AATGAGGTGA GCTCCTGGGT GACATGGCTG
    ATCCTCACGG CGGGCTCCAT GGAGGAGAAG CGAGAAGTCT TCTCCTATTT GGTGCATGTG
    GCCAAATGCT GCTGGAACAT GGGCAACTAC AATGCTGTCA TGGAGTTCTT GGCTGGTCTC
    AGGTCAAGAA AAGTTTTAAA AATGTGGCAG TTTATGGACC AATCTGACCT CGAGACCATG
    AGGAGCCTGA AGGACGCCAT GGCTCAGCAT GAATCTTCCT GCGAATATCG GAAGGTGATA
    ACGAGGGCTC TGCACATACC TGGCTGTAAA GTGGTTCCAT TCTGTGGAGT GTTTCTGAAG
    GAGCTCTGTG AAGTACTGGA CGGAGCCTCT GGCCTCATGA AGCTTTGCCC GAGGTACAAT
    TCCCAAGAAG AAACTCTAGA GTTTGTAGCA GATTACAGTG GACAAGATAA TTTCTTACAA
    CGAGTGGGGC ATAATGGCTT AAAGAATTCA GAGAAGGAGT CCACTGTCAA CAGCATCTTT
    CAAATCATTA GGAGCTGCAG CCGAAGTCTG GAGGCTGAGG AAGAGGACAG CCCCAGTGAA
    GGGAACAGCT CTAGGAAAAA TTCTTTAAGG GACAAAGCCC GATGGCAATT TATAATCGGA
    GATTTGTTGG ATTCCGAAAA TGACATCTTT GAGCAATCCA AAGAATGGGA CTCTCCCAGC
    TCAGAAGAGC CTCAGAAAGC CTTTGACCAT GGGACAGAGC TGATCCCTTG GTACGTGCTG
    TCCATCCGAG CAGATGTGCA CCAGTTCCTG CTACAGGGCG CCACCGTCCT CCGCTACGAC
    CAGGACACGC ACCTCTCTGC CCGCTGCTTC CTCCAGCTTC AGCCTGACAA TAGCACCTTG
    ACATGGATAA AGCCCACAGC TGCCTCCCCG GCCAGTGCTA GAGCAAAACT CGGTGTGCTG
    AGCAACACGT CTGAGCCTGG CAAGTTCCCG TCACCAGGCA GTGCCGGACT AAGCGGCCTG
    GCCGAGGGGG TCCTGGATCT GTTTTCAGCA AAGGCTGTGT ACATGGGACA CCCTAGCATT
    GATATCCACA CTGTGTGTGT CCAGAACAAA CTCGGTAGCA TGTTTCTCTC AGAGACTGGC
    GTGACCCTGC TCTACGGGCT CCAGACCACA GACAATAGAT TATTGCACTT TGTGGCACCC
    AAACACACTG CTAAGATGCT CTTCAGTGGA TTGCTAGAAC TCACGAGGGC CGTGAGGAAG
    ATGAGAAAGT TCCCTGACCA GAGGCAGCAG TGGCTGCGGA AACAGTATGT TAGCCTCTAT
    CAGGAGGACG GACGGTATGA GGGCCCAACT TTGGCTCATG CTGTGGAGTT GTTTGGTGGC
    CGGCGATGGA GTACACGCAA TCCCAGCCCT GGGACATCTG CAAAGAGTGC TGAGAAGCCC
    AGTGTGCAGA GAAACAACAC CTTGGGCATA AGCACCACCA AGAAAAAGAA GAAAATCCTT
    ATAAGGGGTG AGAGTGGAGA GGCAGCCGAC GATGAGATGG CCACCCGGAA GGCCAAAATG
    CACAGGGAGT GTCGCAGCCG GAGTGGTTCT GACCCACAAG ACATGAATGA GCAAGAAGAA
    TCTGAGGCAA ATGCCATCAT GAGCCCTCCT AACACTCTGC CTTCCAGAAG AGCCCACTCT
    TTGACTACAG CTGGGTCCCC TAATTTGTCT GCCGGGACAT CATCTCCCAT CAGGCCAGTG
    TCCTCCCCTG TGCTGTCTTC TTCAAACAAG AGCCCGTCCA GTGCTTGGAG CAGCAGCAGC
    TGGCATGGAA GGATCAAAGG AGGAATGAAG GGTTTTCAGA GCTTCATGGT TTCAGACAGT
    AACATGAGTT TTGTTGAATT TGTTGAGCTG TTCAAATCAT TCAGTGTGAG GAGCCGCAAG
    GACCTGAAGG ATCTCTTTGA CATCTACGCT GTGCCCTGCA ATCGGGCCGG CTCTGAGTCG
    GCCCCGCTCT ACACCAACCT AACAATAGAC GAAAACACCA GCGATCTTCA GCCTGACCTA
    GATTTATTGA CCAGAAACGT CTCGGATTTG GGGTTGTTCA TTAAGAGTAA ACAGCAGCTG
    TCAGACAACC AGAGGCAGAT TTCTGATGCC ATTGCTGCTG CAAGTATCGT GACGAATGGC
    ACTGGGATCG AGAGCACATC TTTGGGCATC TTTGGGGTTG GCATACTCCA GCTCAATGAC
    TTCCTAGTCA ACTGCCAGGG AGAACACTGC ACTTACGATG AAATCCTCAG CATCATCCAG
    AAGTTCGAGC CTAGCATTAG TATGTGTCAT CAGGGCCTAC TGTCCTTTGA AGGATTTGCA
    AGGTTTCTGA TGGATAAAGA TAATTTTGCC TCCAAAAATG ATGAGTCACA GGAGAACATT
    AAAGATTTGC AGCTGCCTCT CTCCTACTAT TACATTGAGT CTTCACACAA TACCTACCTC
    ACAGGCCACC AGCTCAAAGG AGAGTCATCG GTAGAACTCT ATAGCCAGGT CCTCCTGCAA
    GGCTGTAGAA GTGTAGAGTT GGACTGCTGG GATGGAGATG ACGGGATGCC CATCATCTAT
    CACGGACACA CACTAACAAC CAAGATCCCC TTCAAGGAGG TGGTTGAAGC CATCGACCGC
    AGTGCCTTCA TCAACTCTGA CCTGCCCATC ATCATATCGA TTGAGAACCA CTGCTCGTTG
    CCTCAGCAAC GAAAAATGGC AGAAATTTTC AAGACTGTGT TTGGAGAAAA ACTGGTGGCT
    AAATTCCTGT TTGAGAGCGA TTTCTCAGAT GATCCAATGC TTCCCTCACC TGACCAACTT
    AGGAGGAAAG TGCTTCTTAA AAATAAGAAG CTAAAAGCCC ACCAGACACC AGTGGATATC
    TTAAAGCAAA AGGCTCATCA GTTAGCATCC ATGCAAGCTC AGGCTTATAA TGGGGGAAAT
    GTCAACCCTC CACCTGCTAA TAACGAGGAA GAGGAAGATG AAGAGGACGA ATATGATTAT
    GACTATGAAT CCCTGTCTGA TGATGTCCTT ACTGCTTCTC CTGCTCCTAA CAGTCAGGAA
    GACAACATTC TAGAAGACAG ACCTGAAAAT AAATCATGTA ATGACAAGCT TCAGTTTGAG
    TATAACGAAG AAATCCCCAA GAGGATAAAG AAAGCAGATA ATTCTGCTTT CAACAAAGGA
    AAGGTTTATG ACATGGAACT GGGAGAAGAA TTTTATCTTC CTCAGAATAA AAAGGAAAGC
    AGACAGATTG CACCAGAGCT TTCTGACCTT GTCATCTACT GCCAAGCGGT AAAGTTTCCG
    GGGCTGTCAA CTCTAAATGC ATCTGGCTCT AACAGAGGAA AAGAGAGGAA AAGCAGGAAG
    TCCATTTTTG GCAACAATCC GGGCAGAATG AGCCCTGGGG AGACAGCATC GTTTAACAAA
    GCATCTGGAA AAAGTTCCTG TGAAGGAATG CGACAGGCGT GGGAGGAATC TTCTTCCCCC
    CTCAACCCAA CCACATCCCT CAGCGCTATC ATTAGAACTC CCAAATGTTA CCATATCTCA
    TCGCTGAATG AAAATGCCGC CAAACGTCTG TGTCGCAGGT ATTCTCAGAA ACTGATCCAG
    CACACTGCCT GTCAGCTGCT GAGGACTTAC CCGGCTGCCA CACGCATCGA CTCCTCCAAC
    CCCAATCCTC TCTTGTTCTG GCTCCATGGG ATACAGCTCG TGGCACTCAA CTACCAGACA
    GATGATCTCC CTTTACATTT AAATGCTGCC ATGTTTGAGG CCAACGGTGG CTGTGGTTAT
    GTTTTGAAAC CCCCCGTTCT GTGGGACAAG AACTGTCCCA TGTACCAGAA GTTTTCACCC
    TTGGAAAGAG ACCTGGACAA CATGGAGCCT GCCGTCTATT CCTTAACTAT TGTCTCTGGT
    CAAAACGTGT GCCCAAGTAG CAGCACGGGA AGCCCATGTA TCGAAGTGGA CGTGCTGGGC
    ATGCCCCTGG ACAGTTGCCA CTTCCGCACA AAACCCATCC ACCGGAACAC TTTGAACCCC
    ATGTGGAATG AACAGTTTCT CTTCCGGGTT CACTTTGAAG ATCTCATATT CCTGCGTTTT
    GCAGTTGTGG AAAACAATAG CTCAGCCATA ACCGCTCAGA GAATCATCCC CCTCAAGGCT
    TTAAAACGAG GATATCGACA TCTTCAGCTG CGAAACCTCC ACAATGAAGT CTTGGAAATC
    TCTAGTTTAT TCATAAACAG CAGAAGGATG GAAGAAAATT CCTCCAGCAG TGCCACGCCA
    GCCTCTTTGA TGTTTAATAC AGAAGAAAGA AAATGTTTGC AGACTCACAG AGTCACAGTG
    CATGGGGTCC CAGGTCCAGA GCCCTTTACT GTTTTCTCCA TAAGTGGAGG CACGAAGGCA
    AAGCAGCTTC TCCAACAGAT TCTAACTACT GAACAAGACA CCAAACCTAT TGTCACAGAC
    TATTTTTTGA TGGAAGAAAA ATATTTTATA TCTAAAGAAA AGAATGAATG CAGGAAACAA
    CCATTTCAGA GAGTCATCGG TCCAGAAGAA GAGATCATGC AGATTTTAAG CAGCTGGTTT
    CCAGAGGAAG GATATGTTGG CAGGATTGTC TTAAAAACCC AGCAGGAAAA CCTAGAAGAG
    AGAAGCATTG TTCAAGATGA GAAAGAGGTG ATCTTGAGCT CGGAGGAGGA GAGCTTCTTT
    GTCCAAGTGC ATGACGTTTC TCCAGAGCAA CCTCGGACGG TCATCAAAGC GCCCCGTGTC
    AGCACCGCTC AGGATGTCAT TCAACAGACC TTATGCAAAG CCAAATATTC CTATAGCATC
    CTGAGTAACC CCAACCCGAG TGACTATGTG CTTCTGGAAG AGGTGGTGAA AGATGCTACC
    AAGAAGTCCT CCACCCCAAA GTCCTCCCAG CGGGTCCTTT TGGACCAAGA GTGTGTGTTT
    CAAGCCCAGA GCAAGTGGAA AGGTGCTGGA AAATTCATCC TCAGGCTAAA GGAGCAGGTG
    CAGGCATCCC GAGAAGATAA AAGAAAAGGC ATTTCTTTCG CAAGTGAATT CAAGAAGTTC
    ACCAAATCAA CTAAACAGCC CCGAGGACTC ACATCTTCTC AGGTCTTGGC CTCAGAAAGT
    GTCCAAAACA AGGAGGAGAA GCCCACGGGC AGCTTGTCCT CCAGTGACAC CACAGACTCC
    CGACAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013964237.1
    CDS556..7524
    Translation

    Target ORF information:

    RefSeq Version XM_014108762.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris phospholipase C, epsilon 1 (PLCE1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108762.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGACTTCTG AAGGAATGGC AGCTTCTGTT CTCACACCTG TGACTCAAAG AAAAGTGACA 
    TTTGCTCCAT CGGCTGTAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAG TGTTCCAAAG
    GCACATAGTG TCAAGCAGAG TGAGCAGACT TCTACCATCC CGTGGATGAA CAAGCTTAAA
    GAAGAATCTT CTGGAAGCAA CTTGCCCAAA ATTCTCTCCA TAGCAAGGGA GAAAATAGCA
    AGTGATGAGA ACAGCAATGA AGAGTGCTGG ACGGAAAGCA CACCAGTTTC TGTGAAAAAC
    CTTAACATTA ACCATAACAA CATCCTGACA AACCGCCAGT GCGTCCTTCC TCAGAGCCAG
    TCTTATGACA CGTGCAATTC TGTCATGGAG GAAGACCCAT GTTTAGAAAC TGGAATCTCA
    TCCTCACTGG AAAGAAAGGT ATTCCCTGGA ATTCAACTGG AAGTCAACAG ACCTCCCATG
    GACTTTAGGC CTCCAGGCCT AATGGACTTT AGTACCTTAG GAAGTCAGTC AGCCATTGTA
    GATACTGGCC AGGCGCACCC TGACAGCAAC AAAGCAGCAT TTCAAATCTT TAATTATAAA
    GTTGACAGAA GGATGTCTGA CACTTTCTGT ACCCTGTCAG GTGACTTGAT ATTGGATGAC
    TGTGGAAACT GTGTGCCACT GTCATCTGGT TTTGGCGGGG AGCAAAAGAA AAATTACGTG
    GCATATACCT GTAAACTCAT GGAATTGGCT GAAAACTGTG ATAATGAGAA TGGACAGCTG
    CAGTGTGATG ATTATGATGC CTTGGATGAC AAATACTTGT GCTTTGAAGA TTCTTGCCAA
    AGGGACAGTG TGGTATGTTC AAGTGACAGC TTTCGCAGGG AGGACCTAAC TAACAGTCCA
    CCTGCCAAGA CCTTTCTGAG CCATTTTGAA GACTTCCCTG ATAATGGTGA AGATGTAGAA
    GATTTTTTAA AAAACAAAAA GGAGAGATCC ACTTTGTTAG TGAGGAGATT TTGTAAAAAT
    GACAGAGAAG TAAAGAAATC TGTGTATACT GGGACAAGGG CAATCATGAG AACTCTGCCT
    TCTGGTCACA TTGGGCTGGA AGCTTACAGT TACATCGATC AGAAGAGAAG TGGTCCCTTA
    TTACCCCGTG GGAGAGTTCT GGAACAGCTG CCAGTGGTGG CAATTAGGCA AGATGGGAGC
    CAGTGCCTGT CAGAAGCCCA GTGGTATCGG ATCTACAATG CAGTGAGAAG GGAAGAAGAA
    ATAGAAAATA CCATTGGATC TCTTCTCCAT TACTTTACCA AGCTCCCAGC CTCCAAGACA
    GCCCATGAAA GGATTAGTGT TGGTCCATGC CTAAAGCAGT GTGTCCGAGA CACCATATGT
    GAGTATCGTG CCACCCTCCA GAGGACGTCC ATATCTCAGT ACATCACGGG TTCGCTCCTA
    GAAGCAACCA CATCTTTAGG AGCGAGAAGT GGCCTACTCA GTACTTTTGG AGGATCCACT
    GGACGAATGA TGCTAAAAGA ACGCCAGCCA GGCACCTCCA TGGCCAATTC CAGTGCCCTC
    CCTTCAAGTT CCGCTGGAAT CAGCAAGGAG CTGATTGACC TGCAGCCTCT CATTCAGTTC
    CCAGAGGAAG TCGCCAGCAT CCTGATGGAG CAGGAACAGA ACATTTACCG AAGGGTCTTA
    CCAGTTGACT ATCTTTACTT CTTGACCCGG GACTTGGGCA CTCCAGAATG CCAGACACCC
    CTGCCCTGCC TCAAAGCATC CATCTCAGCT TCAATTCTTA GCTCCCAGAA CGGAGAGCAC
    AATGCCCTGG AAGATCTTGT GATGAGATTT AATGAGGTGA GCTCCTGGGT GACATGGCTG
    ATCCTCACGG CGGGCTCCAT GGAGGAGAAG CGAGAAGTCT TCTCCTATTT GGTGCATGTG
    GCCAAATGCT GCTGGAACAT GGGCAACTAC AATGCTGTCA TGGAGTTCTT GGCTGGTCTC
    AGGTCAAGAA AAGTTTTAAA AATGTGGCAG TTTATGGACC AATCTGACCT CGAGACCATG
    AGGAGCCTGA AGGACGCCAT GGCTCAGCAT GAATCTTCCT GCGAATATCG GAAGGTGATA
    ACGAGGGCTC TGCACATACC TGGCTGTAAA GTGGTTCCAT TCTGTGGAGT GTTTCTGAAG
    GAGCTCTGTG AAGTACTGGA CGGAGCCTCT GGCCTCATGA AGCTTTGCCC GAGGTACAAT
    TCCCAAGAAG AAACTCTAGA GTTTGTAGCA GATTACAGTG GACAAGATAA TTTCTTACAA
    CGAGTGGGGC ATAATGGCTT AAAGAATTCA GAGAAGGAGT CCACTGTCAA CAGCATCTTT
    CAAATCATTA GGAGCTGCAG CCGAAGTCTG GAGGCTGAGG AAGAGGACAG CCCCAGTGAA
    GGGAACAGCT CTAGGAAAAA TTCTTTAAGG GACAAAGCCC GATGGCAATT TATAATCGGA
    GATTTGTTGG ATTCCGAAAA TGACATCTTT GAGCAATCCA AAGAATGGGA CTCTCCCAGC
    TCAGAAGAGC CTCAGAAAGC CTTTGACCAT GGGACAGAGC TGATCCCTTG GTACGTGCTG
    TCCATCCGAG CAGATGTGCA CCAGTTCCTG CTACAGGGCG CCACCGTCCT CCGCTACGAC
    CAGGACACGC ACCTCTCTGC CCGCTGCTTC CTCCAGCTTC AGCCTGACAA TAGCACCTTG
    ACATGGATAA AGCCCACAGC TGCCTCCCCG GCCAGTGCTA GAGCAAAACT CGGTGTGCTG
    AGCAACACGT CTGAGCCTGG CAAGTTCCCG TCACCAGGCA GTGCCGGACT AAGCGGCCTG
    GCCGAGGGGG TCCTGGATCT GTTTTCAGCA AAGGCTGTGT ACATGGGACA CCCTAGCATT
    GATATCCACA CTGTGTGTGT CCAGAACAAA CTCGGTAGCA TGTTTCTCTC AGAGACTGGC
    GTGACCCTGC TCTACGGGCT CCAGACCACA GACAATAGAT TATTGCACTT TGTGGCACCC
    AAACACACTG CTAAGATGCT CTTCAGTGGA TTGCTAGAAC TCACGAGGGC CGTGAGGAAG
    ATGAGAAAGT TCCCTGACCA GAGGCAGCAG TGGCTGCGGA AACAGTATGT TAGCCTCTAT
    CAGGAGGACG GACGGTATGA GGGCCCAACT TTGGCTCATG CTGTGGAGTT GTTTGGTGGC
    CGGCGATGGA GTACACGCAA TCCCAGCCCT GGGACATCTG CAAAGAGTGC TGAGAAGCCC
    AGTGTGCAGA GAAACAACAC CTTGGGCATA AGCACCACCA AGAAAAAGAA GAAAATCCTT
    ATAAGGGGTG AGAGTGGAGA GGCAGCCGAC GATGAGATGG CCACCCGGAA GGCCAAAATG
    CACAGGGAGT GTCGCAGCCG GAGTGGTTCT GACCCACAAG ACATGAATGA GCAAGAAGAA
    TCTGAGGCAA ATGCCATCAT GAGCCCTCCT AACACTCTGC CTTCCAGAAG AGCCCACTCT
    TTGACTACAG CTGGGTCCCC TAATTTGTCT GCCGGGACAT CATCTCCCAT CAGGCCAGTG
    TCCTCCCCTG TGCTGTCTTC TTCAAACAAG AGCCCGTCCA GTGCTTGGAG CAGCAGCAGC
    TGGCATGGAA GGATCAAAGG AGGAATGAAG GGTTTTCAGA GCTTCATGGT TTCAGACAGT
    AACATGAGTT TTGTTGAATT TGTTGAGCTG TTCAAATCAT TCAGTGTGAG GAGCCGCAAG
    GACCTGAAGG ATCTCTTTGA CATCTACGCT GTGCCCTGCA ATCGGGCCGG CTCTGAGTCG
    GCCCCGCTCT ACACCAACCT AACAATAGAC GAAAACACCA GCGATCTTCA GCCTGACCTA
    GATTTATTGA CCAGAAACGT CTCGGATTTG GGGTTGTTCA TTAAGAGTAA ACAGCAGCTG
    TCAGACAACC AGAGGCAGAT TTCTGATGCC ATTGCTGCTG CAAGTATCGT GACGAATGGC
    ACTGGGATCG AGAGCACATC TTTGGGCATC TTTGGGGTTG GCATACTCCA GCTCAATGAC
    TTCCTAGTCA ACTGCCAGGG AGAACACTGC ACTTACGATG AAATCCTCAG CATCATCCAG
    AAGTTCGAGC CTAGCATTAG TATGTGTCAT CAGGGCCTAC TGTCCTTTGA AGGATTTGCA
    AGGTTTCTGA TGGATAAAGA TAATTTTGCC TCCAAAAATG ATGAGTCACA GGAGAACATT
    AAAGATTTGC AGCTGCCTCT CTCCTACTAT TACATTGAGT CTTCACACAA TACCTACCTC
    ACAGGCCACC AGCTCAAAGG AGAGTCATCG GTAGAACTCT ATAGCCAGGT CCTCCTGCAA
    GGCTGTAGAA GTGTAGAGTT GGACTGCTGG GATGGAGATG ACGGGATGCC CATCATCTAT
    CACGGACACA CACTAACAAC CAAGATCCCC TTCAAGGAGG TGGTTGAAGC CATCGACCGC
    AGTGCCTTCA TCAACTCTGA CCTGCCCATC ATCATATCGA TTGAGAACCA CTGCTCGTTG
    CCTCAGCAAC GAAAAATGGC AGAAATTTTC AAGACTGTGT TTGGAGAAAA ACTGGTGGCT
    AAATTCCTGT TTGAGAGCGA TTTCTCAGAT GATCCAATGC TTCCCTCACC TGACCAACTT
    AGGAGGAAAG TGCTTCTTAA AAATAAGAAG CTAAAAGCCC ACCAGACACC AGTGGATATC
    TTAAAGCAAA AGGCTCATCA GTTAGCATCC ATGCAAGCTC AGGCTTATAA TGGGGGAAAT
    GTCAACCCTC CACCTGCTAA TAACGAGGAA GAGGAAGATG AAGAGGACGA ATATGATTAT
    GACTATGAAT CCCTGTCTGA TGATGTCCTT ACTGCTTCTC CTGCTCCTAA CAGTCAGGAA
    GACAACATTC TAGAAGACAG ACCTGAAAAT AAATCATGTA ATGACAAGCT TCAGTTTGAG
    TATAACGAAG AAATCCCCAA GAGGATAAAG AAAGCAGATA ATTCTGCTTT CAACAAAGGA
    AAGGTTTATG ACATGGAACT GGGAGAAGAA TTTTATCTTC CTCAGAATAA AAAGGAAAGC
    AGACAGATTG CACCAGAGCT TTCTGACCTT GTCATCTACT GCCAAGCGGT AAAGTTTCCG
    GGGCTGTCAA CTCTAAATGC ATCTGGCTCT AACAGAGGAA AAGAGAGGAA AAGCAGGAAG
    TCCATTTTTG GCAACAATCC GGGCAGAATG AGCCCTGGGG AGACAGCATC GTTTAACAAA
    GCATCTGGAA AAAGTTCCTG TGAAGGAATG CGACAGGCGT GGGAGGAATC TTCTTCCCCC
    CTCAACCCAA CCACATCCCT CAGCGCTATC ATTAGAACTC CCAAATGTTA CCATATCTCA
    TCGCTGAATG AAAATGCCGC CAAACGTCTG TGTCGCAGGT ATTCTCAGAA ACTGATCCAG
    CACACTGCCT GTCAGCTGCT GAGGACTTAC CCGGCTGCCA CACGCATCGA CTCCTCCAAC
    CCCAATCCTC TCTTGTTCTG GCTCCATGGG ATACAGCTCG TGGCACTCAA CTACCAGACA
    GATGATCTCC CTTTACATTT AAATGCTGCC ATGTTTGAGG CCAACGGTGG CTGTGGTTAT
    GTTTTGAAAC CCCCCGTTCT GTGGGACAAG AACTGTCCCA TGTACCAGAA GTTTTCACCC
    TTGGAAAGAG ACCTGGACAA CATGGAGCCT GCCGTCTATT CCTTAACTAT TGTCTCTGGT
    CAAAACGTGT GCCCAAGTAG CAGCACGGGA AGCCCATGTA TCGAAGTGGA CGTGCTGGGC
    ATGCCCCTGG ACAGTTGCCA CTTCCGCACA AAACCCATCC ACCGGAACAC TTTGAACCCC
    ATGTGGAATG AACAGTTTCT CTTCCGGGTT CACTTTGAAG ATCTCATATT CCTGCGTTTT
    GCAGTTGTGG AAAACAATAG CTCAGCCATA ACCGCTCAGA GAATCATCCC CCTCAAGGCT
    TTAAAACGAG GATATCGACA TCTTCAGCTG CGAAACCTCC ACAATGAAGT CTTGGAAATC
    TCTAGTTTAT TCATAAACAG CAGAAGGATG GAAGAAAATT CCTCCAGCAG TGCCACGCCA
    GCCTCTTTGA TGTTTAATAC AGAAGAAAGA AAATGTTTGC AGACTCACAG AGTCACAGTG
    CATGGGGTCC CAGGTCCAGA GCCCTTTACT GTTTTCTCCA TAAGTGGAGG CACGAAGGCA
    AAGCAGCTTC TCCAACAGAT TCTAACTACT GAACAAGACA CCAAACCTAT TGTCACAGAC
    TATTTTTTGA TGGAAGAAAA ATATTTTATA TCTAAAGAAA AGAATGAATG CAGGAAACAA
    CCATTTCAGA GAGTCATCGG TCCAGAAGAA GAGATCATGC AGATTTTAAG CAGCTGGTTT
    CCAGAGGAAG GATATGTTGG CAGGATTGTC TTAAAAACCC AGCAGGAAAA CCTAGAAGAG
    AGAAGCATTG TTCAAGATGA GAAAGAGGTG ATCTTGAGCT CGGAGGAGGA GAGCTTCTTT
    GTCCAAGTGC ATGACGTTTC TCCAGAGCAA CCTCGGACGG TCATCAAAGC GCCCCGTGTC
    AGCACCGCTC AGGATGTCAT TCAACAGACC TTATGCAAAG CCAAATATTC CTATAGCATC
    CTGAGTAACC CCAACCCGAG TGACTATGTG CTTCTGGAAG AGGTGGTGAA AGATGCTACC
    AAGAAGTCCT CCACCCCAAA GTCCTCCCAG CGGGTCCTTT TGGACCAAGA GTGTGTGTTT
    CAAGCCCAGA GCAAGTGGAA AGGTGCTGGA AAATTCATCC TCAGGCTAAA GGAGCAGGTG
    CAGGCATCCC GAGAAGATAA AAGAAAAGGC ATTTCTTTCG CAAGTGAATT CAAGAAGTTC
    ACCAAATCAA CTAAACAGCC CCGAGGACTC ACATCTTCTC AGGTCTTGGC CTCAGAAAGT
    GTCCAAAACA AGGAGGAGAA GCCCACGGGC AGCTTGTCCT CCAGTGACAC CACAGACTCC
    CGACAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe43814
    Clone ID Related Accession (Same CDS sequence) XM_014108762.1 , XM_022411586.1
    Accession Version XM_022411586.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6969bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006610.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGACTTCTG AAGGAATGGC AGCTTCTGTT CTCACACCTG TGACTCAAAG AAAAGTGACA 
    TTTGCTCCAT CGGCTGTAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAG TGTTCCAAAG
    GCACATAGTG TCAAGCAGAG TGAGCAGACT TCTACCATCC CGTGGATGAA CAAGCTTAAA
    GAAGAATCTT CTGGAAGCAA CTTGCCCAAA ATTCTCTCCA TAGCAAGGGA GAAAATAGCA
    AGTGATGAGA ACAGCAATGA AGAGTGCTGG ACGGAAAGCA CACCAGTTTC TGTGAAAAAC
    CTTAACATTA ACCATAACAA CATCCTGACA AACCGCCAGT GCGTCCTTCC TCAGAGCCAG
    TCTTATGACA CGTGCAATTC TGTCATGGAG GAAGACCCAT GTTTAGAAAC TGGAATCTCA
    TCCTCACTGG AAAGAAAGGT ATTCCCTGGA ATTCAACTGG AAGTCAACAG ACCTCCCATG
    GACTTTAGGC CTCCAGGCCT AATGGACTTT AGTACCTTAG GAAGTCAGTC AGCCATTGTA
    GATACTGGCC AGGCGCACCC TGACAGCAAC AAAGCAGCAT TTCAAATCTT TAATTATAAA
    GTTGACAGAA GGATGTCTGA CACTTTCTGT ACCCTGTCAG GTGACTTGAT ATTGGATGAC
    TGTGGAAACT GTGTGCCACT GTCATCTGGT TTTGGCGGGG AGCAAAAGAA AAATTACGTG
    GCATATACCT GTAAACTCAT GGAATTGGCT GAAAACTGTG ATAATGAGAA TGGACAGCTG
    CAGTGTGATG ATTATGATGC CTTGGATGAC AAATACTTGT GCTTTGAAGA TTCTTGCCAA
    AGGGACAGTG TGGTATGTTC AAGTGACAGC TTTCGCAGGG AGGACCTAAC TAACAGTCCA
    CCTGCCAAGA CCTTTCTGAG CCATTTTGAA GACTTCCCTG ATAATGGTGA AGATGTAGAA
    GATTTTTTAA AAAACAAAAA GGAGAGATCC ACTTTGTTAG TGAGGAGATT TTGTAAAAAT
    GACAGAGAAG TAAAGAAATC TGTGTATACT GGGACAAGGG CAATCATGAG AACTCTGCCT
    TCTGGTCACA TTGGGCTGGA AGCTTACAGT TACATCGATC AGAAGAGAAG TGGTCCCTTA
    TTACCCCGTG GGAGAGTTCT GGAACAGCTG CCAGTGGTGG CAATTAGGCA AGATGGGAGC
    CAGTGCCTGT CAGAAGCCCA GTGGTATCGG ATCTACAATG CAGTGAGAAG GGAAGAAGAA
    ATAGAAAATA CCATTGGATC TCTTCTCCAT TACTTTACCA AGCTCCCAGC CTCCAAGACA
    GCCCATGAAA GGATTAGTGT TGGTCCATGC CTAAAGCAGT GTGTCCGAGA CACCATATGT
    GAGTATCGTG CCACCCTCCA GAGGACGTCC ATATCTCAGT ACATCACGGG TTCGCTCCTA
    GAAGCAACCA CATCTTTAGG AGCGAGAAGT GGCCTACTCA GTACTTTTGG AGGATCCACT
    GGACGAATGA TGCTAAAAGA ACGCCAGCCA GGCACCTCCA TGGCCAATTC CAGTGCCCTC
    CCTTCAAGTT CCGCTGGAAT CAGCAAGGAG CTGATTGACC TGCAGCCTCT CATTCAGTTC
    CCAGAGGAAG TCGCCAGCAT CCTGATGGAG CAGGAACAGA ACATTTACCG AAGGGTCTTA
    CCAGTTGACT ATCTTTACTT CTTGACCCGG GACTTGGGCA CTCCAGAATG CCAGACACCC
    CTGCCCTGCC TCAAAGCATC CATCTCAGCT TCAATTCTTA GCTCCCAGAA CGGAGAGCAC
    AATGCCCTGG AAGATCTTGT GATGAGATTT AATGAGGTGA GCTCCTGGGT GACATGGCTG
    ATCCTCACGG CGGGCTCCAT GGAGGAGAAG CGAGAAGTCT TCTCCTATTT GGTGCATGTG
    GCCAAATGCT GCTGGAACAT GGGCAACTAC AATGCTGTCA TGGAGTTCTT GGCTGGTCTC
    AGGTCAAGAA AAGTTTTAAA AATGTGGCAG TTTATGGACC AATCTGACCT CGAGACCATG
    AGGAGCCTGA AGGACGCCAT GGCTCAGCAT GAATCTTCCT GCGAATATCG GAAGGTGATA
    ACGAGGGCTC TGCACATACC TGGCTGTAAA GTGGTTCCAT TCTGTGGAGT GTTTCTGAAG
    GAGCTCTGTG AAGTACTGGA CGGAGCCTCT GGCCTCATGA AGCTTTGCCC GAGGTACAAT
    TCCCAAGAAG AAACTCTAGA GTTTGTAGCA GATTACAGTG GACAAGATAA TTTCTTACAA
    CGAGTGGGGC ATAATGGCTT AAAGAATTCA GAGAAGGAGT CCACTGTCAA CAGCATCTTT
    CAAATCATTA GGAGCTGCAG CCGAAGTCTG GAGGCTGAGG AAGAGGACAG CCCCAGTGAA
    GGGAACAGCT CTAGGAAAAA TTCTTTAAGG GACAAAGCCC GATGGCAATT TATAATCGGA
    GATTTGTTGG ATTCCGAAAA TGACATCTTT GAGCAATCCA AAGAATGGGA CTCTCCCAGC
    TCAGAAGAGC CTCAGAAAGC CTTTGACCAT GGGACAGAGC TGATCCCTTG GTACGTGCTG
    TCCATCCGAG CAGATGTGCA CCAGTTCCTG CTACAGGGCG CCACCGTCCT CCGCTACGAC
    CAGGACACGC ACCTCTCTGC CCGCTGCTTC CTCCAGCTTC AGCCTGACAA TAGCACCTTG
    ACATGGATAA AGCCCACAGC TGCCTCCCCG GCCAGTGCTA GAGCAAAACT CGGTGTGCTG
    AGCAACACGT CTGAGCCTGG CAAGTTCCCG TCACCAGGCA GTGCCGGACT AAGCGGCCTG
    GCCGAGGGGG TCCTGGATCT GTTTTCAGCA AAGGCTGTGT ACATGGGACA CCCTAGCATT
    GATATCCACA CTGTGTGTGT CCAGAACAAA CTCGGTAGCA TGTTTCTCTC AGAGACTGGC
    GTGACCCTGC TCTACGGGCT CCAGACCACA GACAATAGAT TATTGCACTT TGTGGCACCC
    AAACACACTG CTAAGATGCT CTTCAGTGGA TTGCTAGAAC TCACGAGGGC CGTGAGGAAG
    ATGAGAAAGT TCCCTGACCA GAGGCAGCAG TGGCTGCGGA AACAGTATGT TAGCCTCTAT
    CAGGAGGACG GACGGTATGA GGGCCCAACT TTGGCTCATG CTGTGGAGTT GTTTGGTGGC
    CGGCGATGGA GTACACGCAA TCCCAGCCCT GGGACATCTG CAAAGAGTGC TGAGAAGCCC
    AGTGTGCAGA GAAACAACAC CTTGGGCATA AGCACCACCA AGAAAAAGAA GAAAATCCTT
    ATAAGGGGTG AGAGTGGAGA GGCAGCCGAC GATGAGATGG CCACCCGGAA GGCCAAAATG
    CACAGGGAGT GTCGCAGCCG GAGTGGTTCT GACCCACAAG ACATGAATGA GCAAGAAGAA
    TCTGAGGCAA ATGCCATCAT GAGCCCTCCT AACACTCTGC CTTCCAGAAG AGCCCACTCT
    TTGACTACAG CTGGGTCCCC TAATTTGTCT GCCGGGACAT CATCTCCCAT CAGGCCAGTG
    TCCTCCCCTG TGCTGTCTTC TTCAAACAAG AGCCCGTCCA GTGCTTGGAG CAGCAGCAGC
    TGGCATGGAA GGATCAAAGG AGGAATGAAG GGTTTTCAGA GCTTCATGGT TTCAGACAGT
    AACATGAGTT TTGTTGAATT TGTTGAGCTG TTCAAATCAT TCAGTGTGAG GAGCCGCAAG
    GACCTGAAGG ATCTCTTTGA CATCTACGCT GTGCCCTGCA ATCGGGCCGG CTCTGAGTCG
    GCCCCGCTCT ACACCAACCT AACAATAGAC GAAAACACCA GCGATCTTCA GCCTGACCTA
    GATTTATTGA CCAGAAACGT CTCGGATTTG GGGTTGTTCA TTAAGAGTAA ACAGCAGCTG
    TCAGACAACC AGAGGCAGAT TTCTGATGCC ATTGCTGCTG CAAGTATCGT GACGAATGGC
    ACTGGGATCG AGAGCACATC TTTGGGCATC TTTGGGGTTG GCATACTCCA GCTCAATGAC
    TTCCTAGTCA ACTGCCAGGG AGAACACTGC ACTTACGATG AAATCCTCAG CATCATCCAG
    AAGTTCGAGC CTAGCATTAG TATGTGTCAT CAGGGCCTAC TGTCCTTTGA AGGATTTGCA
    AGGTTTCTGA TGGATAAAGA TAATTTTGCC TCCAAAAATG ATGAGTCACA GGAGAACATT
    AAAGATTTGC AGCTGCCTCT CTCCTACTAT TACATTGAGT CTTCACACAA TACCTACCTC
    ACAGGCCACC AGCTCAAAGG AGAGTCATCG GTAGAACTCT ATAGCCAGGT CCTCCTGCAA
    GGCTGTAGAA GTGTAGAGTT GGACTGCTGG GATGGAGATG ACGGGATGCC CATCATCTAT
    CACGGACACA CACTAACAAC CAAGATCCCC TTCAAGGAGG TGGTTGAAGC CATCGACCGC
    AGTGCCTTCA TCAACTCTGA CCTGCCCATC ATCATATCGA TTGAGAACCA CTGCTCGTTG
    CCTCAGCAAC GAAAAATGGC AGAAATTTTC AAGACTGTGT TTGGAGAAAA ACTGGTGGCT
    AAATTCCTGT TTGAGAGCGA TTTCTCAGAT GATCCAATGC TTCCCTCACC TGACCAACTT
    AGGAGGAAAG TGCTTCTTAA AAATAAGAAG CTAAAAGCCC ACCAGACACC AGTGGATATC
    TTAAAGCAAA AGGCTCATCA GTTAGCATCC ATGCAAGCTC AGGCTTATAA TGGGGGAAAT
    GTCAACCCTC CACCTGCTAA TAACGAGGAA GAGGAAGATG AAGAGGACGA ATATGATTAT
    GACTATGAAT CCCTGTCTGA TGATGTCCTT ACTGCTTCTC CTGCTCCTAA CAGTCAGGAA
    GACAACATTC TAGAAGACAG ACCTGAAAAT AAATCATGTA ATGACAAGCT TCAGTTTGAG
    TATAACGAAG AAATCCCCAA GAGGATAAAG AAAGCAGATA ATTCTGCTTT CAACAAAGGA
    AAGGTTTATG ACATGGAACT GGGAGAAGAA TTTTATCTTC CTCAGAATAA AAAGGAAAGC
    AGACAGATTG CACCAGAGCT TTCTGACCTT GTCATCTACT GCCAAGCGGT AAAGTTTCCG
    GGGCTGTCAA CTCTAAATGC ATCTGGCTCT AACAGAGGAA AAGAGAGGAA AAGCAGGAAG
    TCCATTTTTG GCAACAATCC GGGCAGAATG AGCCCTGGGG AGACAGCATC GTTTAACAAA
    GCATCTGGAA AAAGTTCCTG TGAAGGAATG CGACAGGCGT GGGAGGAATC TTCTTCCCCC
    CTCAACCCAA CCACATCCCT CAGCGCTATC ATTAGAACTC CCAAATGTTA CCATATCTCA
    TCGCTGAATG AAAATGCCGC CAAACGTCTG TGTCGCAGGT ATTCTCAGAA ACTGATCCAG
    CACACTGCCT GTCAGCTGCT GAGGACTTAC CCGGCTGCCA CACGCATCGA CTCCTCCAAC
    CCCAATCCTC TCTTGTTCTG GCTCCATGGG ATACAGCTCG TGGCACTCAA CTACCAGACA
    GATGATCTCC CTTTACATTT AAATGCTGCC ATGTTTGAGG CCAACGGTGG CTGTGGTTAT
    GTTTTGAAAC CCCCCGTTCT GTGGGACAAG AACTGTCCCA TGTACCAGAA GTTTTCACCC
    TTGGAAAGAG ACCTGGACAA CATGGAGCCT GCCGTCTATT CCTTAACTAT TGTCTCTGGT
    CAAAACGTGT GCCCAAGTAG CAGCACGGGA AGCCCATGTA TCGAAGTGGA CGTGCTGGGC
    ATGCCCCTGG ACAGTTGCCA CTTCCGCACA AAACCCATCC ACCGGAACAC TTTGAACCCC
    ATGTGGAATG AACAGTTTCT CTTCCGGGTT CACTTTGAAG ATCTCATATT CCTGCGTTTT
    GCAGTTGTGG AAAACAATAG CTCAGCCATA ACCGCTCAGA GAATCATCCC CCTCAAGGCT
    TTAAAACGAG GATATCGACA TCTTCAGCTG CGAAACCTCC ACAATGAAGT CTTGGAAATC
    TCTAGTTTAT TCATAAACAG CAGAAGGATG GAAGAAAATT CCTCCAGCAG TGCCACGCCA
    GCCTCTTTGA TGTTTAATAC AGAAGAAAGA AAATGTTTGC AGACTCACAG AGTCACAGTG
    CATGGGGTCC CAGGTCCAGA GCCCTTTACT GTTTTCTCCA TAAGTGGAGG CACGAAGGCA
    AAGCAGCTTC TCCAACAGAT TCTAACTACT GAACAAGACA CCAAACCTAT TGTCACAGAC
    TATTTTTTGA TGGAAGAAAA ATATTTTATA TCTAAAGAAA AGAATGAATG CAGGAAACAA
    CCATTTCAGA GAGTCATCGG TCCAGAAGAA GAGATCATGC AGATTTTAAG CAGCTGGTTT
    CCAGAGGAAG GATATGTTGG CAGGATTGTC TTAAAAACCC AGCAGGAAAA CCTAGAAGAG
    AGAAGCATTG TTCAAGATGA GAAAGAGGTG ATCTTGAGCT CGGAGGAGGA GAGCTTCTTT
    GTCCAAGTGC ATGACGTTTC TCCAGAGCAA CCTCGGACGG TCATCAAAGC GCCCCGTGTC
    AGCACCGCTC AGGATGTCAT TCAACAGACC TTATGCAAAG CCAAATATTC CTATAGCATC
    CTGAGTAACC CCAACCCGAG TGACTATGTG CTTCTGGAAG AGGTGGTGAA AGATGCTACC
    AAGAAGTCCT CCACCCCAAA GTCCTCCCAG CGGGTCCTTT TGGACCAAGA GTGTGTGTTT
    CAAGCCCAGA GCAAGTGGAA AGGTGCTGGA AAATTCATCC TCAGGCTAAA GGAGCAGGTG
    CAGGCATCCC GAGAAGATAA AAGAAAAGGC ATTTCTTTCG CAAGTGAATT CAAGAAGTTC
    ACCAAATCAA CTAAACAGCC CCGAGGACTC ACATCTTCTC AGGTCTTGGC CTCAGAAAGT
    GTCCAAAACA AGGAGGAGAA GCCCACGGGC AGCTTGTCCT CCAGTGACAC CACAGACTCC
    CGACAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022267294.1
    CDS352..7320
    Translation

    Target ORF information:

    RefSeq Version XM_022411586.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris phospholipase C epsilon 1 (PLCE1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022411586.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGACTTCTG AAGGAATGGC AGCTTCTGTT CTCACACCTG TGACTCAAAG AAAAGTGACA 
    TTTGCTCCAT CGGCTGTAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAG TGTTCCAAAG
    GCACATAGTG TCAAGCAGAG TGAGCAGACT TCTACCATCC CGTGGATGAA CAAGCTTAAA
    GAAGAATCTT CTGGAAGCAA CTTGCCCAAA ATTCTCTCCA TAGCAAGGGA GAAAATAGCA
    AGTGATGAGA ACAGCAATGA AGAGTGCTGG ACGGAAAGCA CACCAGTTTC TGTGAAAAAC
    CTTAACATTA ACCATAACAA CATCCTGACA AACCGCCAGT GCGTCCTTCC TCAGAGCCAG
    TCTTATGACA CGTGCAATTC TGTCATGGAG GAAGACCCAT GTTTAGAAAC TGGAATCTCA
    TCCTCACTGG AAAGAAAGGT ATTCCCTGGA ATTCAACTGG AAGTCAACAG ACCTCCCATG
    GACTTTAGGC CTCCAGGCCT AATGGACTTT AGTACCTTAG GAAGTCAGTC AGCCATTGTA
    GATACTGGCC AGGCGCACCC TGACAGCAAC AAAGCAGCAT TTCAAATCTT TAATTATAAA
    GTTGACAGAA GGATGTCTGA CACTTTCTGT ACCCTGTCAG GTGACTTGAT ATTGGATGAC
    TGTGGAAACT GTGTGCCACT GTCATCTGGT TTTGGCGGGG AGCAAAAGAA AAATTACGTG
    GCATATACCT GTAAACTCAT GGAATTGGCT GAAAACTGTG ATAATGAGAA TGGACAGCTG
    CAGTGTGATG ATTATGATGC CTTGGATGAC AAATACTTGT GCTTTGAAGA TTCTTGCCAA
    AGGGACAGTG TGGTATGTTC AAGTGACAGC TTTCGCAGGG AGGACCTAAC TAACAGTCCA
    CCTGCCAAGA CCTTTCTGAG CCATTTTGAA GACTTCCCTG ATAATGGTGA AGATGTAGAA
    GATTTTTTAA AAAACAAAAA GGAGAGATCC ACTTTGTTAG TGAGGAGATT TTGTAAAAAT
    GACAGAGAAG TAAAGAAATC TGTGTATACT GGGACAAGGG CAATCATGAG AACTCTGCCT
    TCTGGTCACA TTGGGCTGGA AGCTTACAGT TACATCGATC AGAAGAGAAG TGGTCCCTTA
    TTACCCCGTG GGAGAGTTCT GGAACAGCTG CCAGTGGTGG CAATTAGGCA AGATGGGAGC
    CAGTGCCTGT CAGAAGCCCA GTGGTATCGG ATCTACAATG CAGTGAGAAG GGAAGAAGAA
    ATAGAAAATA CCATTGGATC TCTTCTCCAT TACTTTACCA AGCTCCCAGC CTCCAAGACA
    GCCCATGAAA GGATTAGTGT TGGTCCATGC CTAAAGCAGT GTGTCCGAGA CACCATATGT
    GAGTATCGTG CCACCCTCCA GAGGACGTCC ATATCTCAGT ACATCACGGG TTCGCTCCTA
    GAAGCAACCA CATCTTTAGG AGCGAGAAGT GGCCTACTCA GTACTTTTGG AGGATCCACT
    GGACGAATGA TGCTAAAAGA ACGCCAGCCA GGCACCTCCA TGGCCAATTC CAGTGCCCTC
    CCTTCAAGTT CCGCTGGAAT CAGCAAGGAG CTGATTGACC TGCAGCCTCT CATTCAGTTC
    CCAGAGGAAG TCGCCAGCAT CCTGATGGAG CAGGAACAGA ACATTTACCG AAGGGTCTTA
    CCAGTTGACT ATCTTTACTT CTTGACCCGG GACTTGGGCA CTCCAGAATG CCAGACACCC
    CTGCCCTGCC TCAAAGCATC CATCTCAGCT TCAATTCTTA GCTCCCAGAA CGGAGAGCAC
    AATGCCCTGG AAGATCTTGT GATGAGATTT AATGAGGTGA GCTCCTGGGT GACATGGCTG
    ATCCTCACGG CGGGCTCCAT GGAGGAGAAG CGAGAAGTCT TCTCCTATTT GGTGCATGTG
    GCCAAATGCT GCTGGAACAT GGGCAACTAC AATGCTGTCA TGGAGTTCTT GGCTGGTCTC
    AGGTCAAGAA AAGTTTTAAA AATGTGGCAG TTTATGGACC AATCTGACCT CGAGACCATG
    AGGAGCCTGA AGGACGCCAT GGCTCAGCAT GAATCTTCCT GCGAATATCG GAAGGTGATA
    ACGAGGGCTC TGCACATACC TGGCTGTAAA GTGGTTCCAT TCTGTGGAGT GTTTCTGAAG
    GAGCTCTGTG AAGTACTGGA CGGAGCCTCT GGCCTCATGA AGCTTTGCCC GAGGTACAAT
    TCCCAAGAAG AAACTCTAGA GTTTGTAGCA GATTACAGTG GACAAGATAA TTTCTTACAA
    CGAGTGGGGC ATAATGGCTT AAAGAATTCA GAGAAGGAGT CCACTGTCAA CAGCATCTTT
    CAAATCATTA GGAGCTGCAG CCGAAGTCTG GAGGCTGAGG AAGAGGACAG CCCCAGTGAA
    GGGAACAGCT CTAGGAAAAA TTCTTTAAGG GACAAAGCCC GATGGCAATT TATAATCGGA
    GATTTGTTGG ATTCCGAAAA TGACATCTTT GAGCAATCCA AAGAATGGGA CTCTCCCAGC
    TCAGAAGAGC CTCAGAAAGC CTTTGACCAT GGGACAGAGC TGATCCCTTG GTACGTGCTG
    TCCATCCGAG CAGATGTGCA CCAGTTCCTG CTACAGGGCG CCACCGTCCT CCGCTACGAC
    CAGGACACGC ACCTCTCTGC CCGCTGCTTC CTCCAGCTTC AGCCTGACAA TAGCACCTTG
    ACATGGATAA AGCCCACAGC TGCCTCCCCG GCCAGTGCTA GAGCAAAACT CGGTGTGCTG
    AGCAACACGT CTGAGCCTGG CAAGTTCCCG TCACCAGGCA GTGCCGGACT AAGCGGCCTG
    GCCGAGGGGG TCCTGGATCT GTTTTCAGCA AAGGCTGTGT ACATGGGACA CCCTAGCATT
    GATATCCACA CTGTGTGTGT CCAGAACAAA CTCGGTAGCA TGTTTCTCTC AGAGACTGGC
    GTGACCCTGC TCTACGGGCT CCAGACCACA GACAATAGAT TATTGCACTT TGTGGCACCC
    AAACACACTG CTAAGATGCT CTTCAGTGGA TTGCTAGAAC TCACGAGGGC CGTGAGGAAG
    ATGAGAAAGT TCCCTGACCA GAGGCAGCAG TGGCTGCGGA AACAGTATGT TAGCCTCTAT
    CAGGAGGACG GACGGTATGA GGGCCCAACT TTGGCTCATG CTGTGGAGTT GTTTGGTGGC
    CGGCGATGGA GTACACGCAA TCCCAGCCCT GGGACATCTG CAAAGAGTGC TGAGAAGCCC
    AGTGTGCAGA GAAACAACAC CTTGGGCATA AGCACCACCA AGAAAAAGAA GAAAATCCTT
    ATAAGGGGTG AGAGTGGAGA GGCAGCCGAC GATGAGATGG CCACCCGGAA GGCCAAAATG
    CACAGGGAGT GTCGCAGCCG GAGTGGTTCT GACCCACAAG ACATGAATGA GCAAGAAGAA
    TCTGAGGCAA ATGCCATCAT GAGCCCTCCT AACACTCTGC CTTCCAGAAG AGCCCACTCT
    TTGACTACAG CTGGGTCCCC TAATTTGTCT GCCGGGACAT CATCTCCCAT CAGGCCAGTG
    TCCTCCCCTG TGCTGTCTTC TTCAAACAAG AGCCCGTCCA GTGCTTGGAG CAGCAGCAGC
    TGGCATGGAA GGATCAAAGG AGGAATGAAG GGTTTTCAGA GCTTCATGGT TTCAGACAGT
    AACATGAGTT TTGTTGAATT TGTTGAGCTG TTCAAATCAT TCAGTGTGAG GAGCCGCAAG
    GACCTGAAGG ATCTCTTTGA CATCTACGCT GTGCCCTGCA ATCGGGCCGG CTCTGAGTCG
    GCCCCGCTCT ACACCAACCT AACAATAGAC GAAAACACCA GCGATCTTCA GCCTGACCTA
    GATTTATTGA CCAGAAACGT CTCGGATTTG GGGTTGTTCA TTAAGAGTAA ACAGCAGCTG
    TCAGACAACC AGAGGCAGAT TTCTGATGCC ATTGCTGCTG CAAGTATCGT GACGAATGGC
    ACTGGGATCG AGAGCACATC TTTGGGCATC TTTGGGGTTG GCATACTCCA GCTCAATGAC
    TTCCTAGTCA ACTGCCAGGG AGAACACTGC ACTTACGATG AAATCCTCAG CATCATCCAG
    AAGTTCGAGC CTAGCATTAG TATGTGTCAT CAGGGCCTAC TGTCCTTTGA AGGATTTGCA
    AGGTTTCTGA TGGATAAAGA TAATTTTGCC TCCAAAAATG ATGAGTCACA GGAGAACATT
    AAAGATTTGC AGCTGCCTCT CTCCTACTAT TACATTGAGT CTTCACACAA TACCTACCTC
    ACAGGCCACC AGCTCAAAGG AGAGTCATCG GTAGAACTCT ATAGCCAGGT CCTCCTGCAA
    GGCTGTAGAA GTGTAGAGTT GGACTGCTGG GATGGAGATG ACGGGATGCC CATCATCTAT
    CACGGACACA CACTAACAAC CAAGATCCCC TTCAAGGAGG TGGTTGAAGC CATCGACCGC
    AGTGCCTTCA TCAACTCTGA CCTGCCCATC ATCATATCGA TTGAGAACCA CTGCTCGTTG
    CCTCAGCAAC GAAAAATGGC AGAAATTTTC AAGACTGTGT TTGGAGAAAA ACTGGTGGCT
    AAATTCCTGT TTGAGAGCGA TTTCTCAGAT GATCCAATGC TTCCCTCACC TGACCAACTT
    AGGAGGAAAG TGCTTCTTAA AAATAAGAAG CTAAAAGCCC ACCAGACACC AGTGGATATC
    TTAAAGCAAA AGGCTCATCA GTTAGCATCC ATGCAAGCTC AGGCTTATAA TGGGGGAAAT
    GTCAACCCTC CACCTGCTAA TAACGAGGAA GAGGAAGATG AAGAGGACGA ATATGATTAT
    GACTATGAAT CCCTGTCTGA TGATGTCCTT ACTGCTTCTC CTGCTCCTAA CAGTCAGGAA
    GACAACATTC TAGAAGACAG ACCTGAAAAT AAATCATGTA ATGACAAGCT TCAGTTTGAG
    TATAACGAAG AAATCCCCAA GAGGATAAAG AAAGCAGATA ATTCTGCTTT CAACAAAGGA
    AAGGTTTATG ACATGGAACT GGGAGAAGAA TTTTATCTTC CTCAGAATAA AAAGGAAAGC
    AGACAGATTG CACCAGAGCT TTCTGACCTT GTCATCTACT GCCAAGCGGT AAAGTTTCCG
    GGGCTGTCAA CTCTAAATGC ATCTGGCTCT AACAGAGGAA AAGAGAGGAA AAGCAGGAAG
    TCCATTTTTG GCAACAATCC GGGCAGAATG AGCCCTGGGG AGACAGCATC GTTTAACAAA
    GCATCTGGAA AAAGTTCCTG TGAAGGAATG CGACAGGCGT GGGAGGAATC TTCTTCCCCC
    CTCAACCCAA CCACATCCCT CAGCGCTATC ATTAGAACTC CCAAATGTTA CCATATCTCA
    TCGCTGAATG AAAATGCCGC CAAACGTCTG TGTCGCAGGT ATTCTCAGAA ACTGATCCAG
    CACACTGCCT GTCAGCTGCT GAGGACTTAC CCGGCTGCCA CACGCATCGA CTCCTCCAAC
    CCCAATCCTC TCTTGTTCTG GCTCCATGGG ATACAGCTCG TGGCACTCAA CTACCAGACA
    GATGATCTCC CTTTACATTT AAATGCTGCC ATGTTTGAGG CCAACGGTGG CTGTGGTTAT
    GTTTTGAAAC CCCCCGTTCT GTGGGACAAG AACTGTCCCA TGTACCAGAA GTTTTCACCC
    TTGGAAAGAG ACCTGGACAA CATGGAGCCT GCCGTCTATT CCTTAACTAT TGTCTCTGGT
    CAAAACGTGT GCCCAAGTAG CAGCACGGGA AGCCCATGTA TCGAAGTGGA CGTGCTGGGC
    ATGCCCCTGG ACAGTTGCCA CTTCCGCACA AAACCCATCC ACCGGAACAC TTTGAACCCC
    ATGTGGAATG AACAGTTTCT CTTCCGGGTT CACTTTGAAG ATCTCATATT CCTGCGTTTT
    GCAGTTGTGG AAAACAATAG CTCAGCCATA ACCGCTCAGA GAATCATCCC CCTCAAGGCT
    TTAAAACGAG GATATCGACA TCTTCAGCTG CGAAACCTCC ACAATGAAGT CTTGGAAATC
    TCTAGTTTAT TCATAAACAG CAGAAGGATG GAAGAAAATT CCTCCAGCAG TGCCACGCCA
    GCCTCTTTGA TGTTTAATAC AGAAGAAAGA AAATGTTTGC AGACTCACAG AGTCACAGTG
    CATGGGGTCC CAGGTCCAGA GCCCTTTACT GTTTTCTCCA TAAGTGGAGG CACGAAGGCA
    AAGCAGCTTC TCCAACAGAT TCTAACTACT GAACAAGACA CCAAACCTAT TGTCACAGAC
    TATTTTTTGA TGGAAGAAAA ATATTTTATA TCTAAAGAAA AGAATGAATG CAGGAAACAA
    CCATTTCAGA GAGTCATCGG TCCAGAAGAA GAGATCATGC AGATTTTAAG CAGCTGGTTT
    CCAGAGGAAG GATATGTTGG CAGGATTGTC TTAAAAACCC AGCAGGAAAA CCTAGAAGAG
    AGAAGCATTG TTCAAGATGA GAAAGAGGTG ATCTTGAGCT CGGAGGAGGA GAGCTTCTTT
    GTCCAAGTGC ATGACGTTTC TCCAGAGCAA CCTCGGACGG TCATCAAAGC GCCCCGTGTC
    AGCACCGCTC AGGATGTCAT TCAACAGACC TTATGCAAAG CCAAATATTC CTATAGCATC
    CTGAGTAACC CCAACCCGAG TGACTATGTG CTTCTGGAAG AGGTGGTGAA AGATGCTACC
    AAGAAGTCCT CCACCCCAAA GTCCTCCCAG CGGGTCCTTT TGGACCAAGA GTGTGTGTTT
    CAAGCCCAGA GCAAGTGGAA AGGTGCTGGA AAATTCATCC TCAGGCTAAA GGAGCAGGTG
    CAGGCATCCC GAGAAGATAA AAGAAAAGGC ATTTCTTTCG CAAGTGAATT CAAGAAGTTC
    ACCAAATCAA CTAAACAGCC CCGAGGACTC ACATCTTCTC AGGTCTTGGC CTCAGAAAGT
    GTCCAAAACA AGGAGGAGAA GCCCACGGGC AGCTTGTCCT CCAGTGACAC CACAGACTCC
    CGACAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27696
    Clone ID Related Accession (Same CDS sequence) XM_022411587.1 , NM_001136565.1 , XM_005637459.2
    Accession Version NM_001136565.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6930bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Canis lupus familiaris(dog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAEX03015425.1, AAEX03015426.1 and AAEX03015427.1. On or before Nov 13, 2008 this sequence version replaced XM_543937.2, XM_853995.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA103936002 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGACTTCTG AAGGAATGGC AGCTTCTGTT CTCACACCTG TGACTCAAAG AAAAGTGACA 
    TTTGCTCCAT CGGCTGTAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAG TGTTCCAAAG
    GCACATAGTG TCAAGCAGAG TGAGCAGACT TCTACCATCC CGTGGATGAA CAAGCTTAAA
    GAAGAATCTT CTGGAAGCAA CTTGCCCAAA ATTCTCTCCA TAGCAAGGGA GAAAATAGCA
    AGTGATGAGA ACAGCAATGA AGAGTGCTGG ACGGAAAGCA CACCAGTTTC TGTGAAAAAC
    CTTAACATTA ACCATAACAA CATCCTGACA AACCGCCAGT GCGTCCTTCC TCAGAGCCAG
    TCTTATGACA CGTGCAATTC TGTCATGGAG GAAGACCCAT GTTTAGAAAC TGGAATCTCA
    TCCTCACTGG AAAGAAAGGT ATTCCCTGGA ATTCAACTGG AAGTCAACAG ACCTCCCATG
    GACTTTAGGC CTCCAGGCCT AATGGACTTT AGTACCTTAG GAAGTCAGTC AGCCATTGTA
    GATACTGGCC AGGCGCACCC TGACAGCAAC AAAGCAGCAT TTCAAATCTT TAATTATAAA
    GTTGACAGAA GGATGTCTGA CACTTTCTGT ACCCTGTCAG GTGACTTGAT ATTGGATGAC
    TGTGGAAACT GTGTGCCACT GTCATCTGGT TTTGGCGGGG AGCAAAAGAA AAATTACGTG
    GCATATACCT GTAAACTCAT GGAATTGGCT GAAAACTGTG ATAATGAGAA TGGACAGCTG
    CAGTGTGATG ATTATGATGC CTTGGATGAC AAATACTTGT GCTTTGAAGA TTCTTGCCAA
    AGGGACAGTG TGGTATGTTC AAGTGACAGC TTTCGCAGGG AGGACCTAAC TAACAGTCCA
    CCTGCCAAGA CCTTTCTGAG CCATTTTGAA GACTTCCCTG ATAATGGTGA AGATGTAGAA
    GATTTTTTAA AAAACAAAAA GGAGAGATCC ACTTTGTTAG TGAGGAGATT TTGTAAAAAT
    GACAGAGAAG TAAAGAAATC TGTGTATACT GGGACAAGGG CAATCATGAG AACTCTGCCT
    TCTGGTCACA TTGGGCTGGA AGCTTACAGT TACATCGATC AGAAGAGAAG TGGTCCCTTA
    TTACCCCGTG GGAGAGTTCT GGAACAGCTG CCAGTGGTGG CAATTAGGCA AGATGGGAGC
    CAGTGCCTGT CAGAAGCCCA GTGGTATCGG ATCTACAATG CAGTGAGAAG GGAAGAAGAA
    ATAGAAAATA CCATTGGATC TCTTCTCCAT TACTTTACCA AGCTCCCAGC CTCCAAGACA
    GCCCATGAAA GGATTAGTGT TGGTCCATGC CTAAAGCAGT GTGTCCGAGA CACCATATGT
    GAGTATCGTG CCACCCTCCA GAGGACGTCC ATATCTCAGT ACATCACGGG TTCGCTCCTA
    GAAGCAACCA CATCTTTAGG AGCGAGAAGT GGCCTACTCA GTACTTTTGG AGGATCCACT
    GGACGAATGA TGCTAAAAGA ACGCCAGCCA GGCACCTCCA TGGCCAATTC CAGTGCCCTC
    CCTTCAAGTT CCGCTGGAAT CAGCAAGGAG CTGATTGACC TGCAGCCTCT CATTCAGTTC
    CCAGAGGAAG TCGCCAGCAT CCTGATGGAG CAGGAACAGA ACATTTACCG AAGGGTCTTA
    CCAGTTGACT ATCTTTACTT CTTGACCCGG GACTTGGGCA CTCCAGAATG CCAGACACCC
    CTGCCCTGCC TCAAAGCATC CATCTCAGCT TCAATTCTTA GCTCCCAGAA CGGAGAGCAC
    AATGCCCTGG AAGATCTTGT GATGAGATTT AATGAGGTGA GCTCCTGGGT GACATGGCTG
    ATCCTCACGG CGGGCTCCAT GGAGGAGAAG CGAGAAGTCT TCTCCTATTT GGTGCATGTG
    GCCAAATGCT GCTGGAACAT GGGCAACTAC AATGCTGTCA TGGAGTTCTT GGCTGGTCTC
    AGGTCAAGAA AAGTTTTAAA AATGTGGCAG TTTATGGACC AATCTGACCT CGAGACCATG
    AGGAGCCTGA AGGACGCCAT GGCTCAGCAT GAATCTTCCT GCGAATATCG GAAGGTGATA
    ACGAGGGCTC TGCACATACC TGGCTGTAAA GTGGTTCCAT TCTGTGGAGT GTTTCTGAAG
    GAGCTCTGTG AAGTACTGGA CGGAGCCTCT GGCCTCATGA AGCTTTGCCC GAGGTACAAT
    TCCCAAGAAG AAACTCTAGA GTTTGTAGCA GATTACAGTG GACAAGATAA TTTCTTACAA
    CGAGTGGGGC ATAATGGCTT AAAGAATTCA GAGAAGGAGT CCACTGTCAA CAGCATCTTT
    CAAATCATTA GGAGCTGCAG CCGAAGTCTG GAGGCTGAGG AAGAGGACAG CCCCAGTGAA
    GGGAACAGCT CTAGGAAAAA TTCTTTAAGG GACAAAGCCC GATGGCAATT TATAATCGGA
    GATTTGTTGG ATTCCGAAAA TGACATCTTT GAGCAATCCA AAGAATGGGA CTCTCCCAGC
    TCAGAAGAGC CTCAGAAAGC CTTTGACCAT GGGACAGAGC TGATCCCTTG GTACGTGCTG
    TCCATCCGAG CAGATGTGCA CCAGTTCCTG CTACAGGGCG CCACCGTCCT CCGCTACGAC
    CAGGACACGC ACCTCTCTGC CCGCTGCTTC CTCCAGCTTC AGCCTGACAA TAGCACCTTG
    ACATGGATAA AGCCCACAGC TGCCTCCCCG GCCAGTGCTA GAGCAAAACT CGGTGTGCTG
    AGCAACACGT CTGAGCCTGG CAAGTTCCCG TCACCAGGCA GTGCCGGACT AAGCGGCCTG
    GCCGAGGGGG TCCTGGATCT GTTTTCAGCA AAGGCTGTGT ACATGGGACA CCCTAGCATT
    GATATCCACA CTGTGTGTGT CCAGAACAAA CTCGGTAGCA TGTTTCTCTC AGAGACTGGC
    GTGACCCTGC TCTACGGGCT CCAGACCACA GACAATAGAT TATTGCACTT TGTGGCACCC
    AAACACACTG CTAAGATGCT CTTCAGTGGA TTGCTAGAAC TCACGAGGGC CGTGAGGAAG
    ATGAGAAAGT TCCCTGACCA GAGGCAGCAG TGGCTGCGGA AACAGTATGT TAGCCTCTAT
    CAGGAGGACG GACGGTATGA GGGCCCAACT TTGGCTCATG CTGTGGAGTT GTTTGGTGGC
    CGGCGATGGA GTACACGCAA TCCCAGCCCT GGGACATCTG CAAAGAGTGC TGAGAAGCCC
    AGTGTGCAGA GAAACAACAC CTTGGGCATA AGCACCACCA AGAAAAAGAA GAAAATCCTT
    ATAAGGGGTG AGAGTGGAGA GGCAGCCGAC GATGAGATGG CCACCCGGAA GGCCAAAATG
    CACAGGGAGT GTCGCAGCCG GAGTGGTTCT GACCCACAAG ACATGAATGA GCAAGAAGAA
    TCTGAGGCAA ATGCCATCAT GAGCCCTCCT AACACTCTGC CTTCCAGAAG AGCCCACTCT
    TTGACTACAG CTGGGTCCCC TAATTTGTCT GCCGGGACAT CATCTCCCAT CAGGCCAGTG
    TCCTCCCCTG TGCTGTCTTC TTCAAACAAG AGCCCGTCCA GTGCTTGGAG CAGCAGCAGC
    TGGCATGGAA GGATCAAAGG AGGAATGAAG GGTTTTCAGA GCTTCATGGT TTCAGACAGT
    AACATGAGTT TTGTTGAATT TGTTGAGCTG TTCAAATCAT TCAGTGTGAG GAGCCGCAAG
    GACCTGAAGG ATCTCTTTGA CATCTACGCT GTGCCCTGCA ATCGGGCCGG CTCTGAGTCG
    GCCCCGCTCT ACACCAACCT AACAATAGAC GAAAACACCA GCGATCTTCA GCCTGACCTA
    GATTTATTGA CCAGAAACGT CTCGGATTTG GGGTTGTTCA TTAAGAGTAA ACAGCAGCTG
    TCAGACAACC AGAGGCAGAT TTCTGATGCC ATTGCTGCTG CAAGTATCGT GACGAATGGC
    ACTGGGATCG AGAGCACATC TTTGGGCATC TTTGGGGTTG GCATACTCCA GCTCAATGAC
    TTCCTAGTCA ACTGCCAGGG AGAACACTGC ACTTACGATG AAATCCTCAG CATCATCCAG
    AAGTTCGAGC CTAGCATTAG TATGTGTCAT CAGGGCCTAC TGTCCTTTGA AGGATTTGCA
    AGGTTTCTGA TGGATAAAGA TAATTTTGCC TCCAAAAATG ATGAGTCACA GGAGAACATT
    AAAGATTTGC AGCTGCCTCT CTCCTACTAT TACATTGAGT CTTCACACAA TACCTACCTC
    ACAGGCCACC AGCTCAAAGG AGAGTCATCG GTAGAACTCT ATAGCCAGGT CCTCCTGCAA
    GGCTGTAGAA GTGTAGAGTT GGACTGCTGG GATGGAGATG ACGGGATGCC CATCATCTAT
    CACGGACACA CACTAACAAC CAAGATCCCC TTCAAGGAGG TGGTTGAAGC CATCGACCGC
    AGTGCCTTCA TCAACTCTGA CCTGCCCATC ATCATATCGA TTGAGAACCA CTGCTCGTTG
    CCTCAGCAAC GAAAAATGGC AGAAATTTTC AAGACTGTGT TTGGAGAAAA ACTGGTGGCT
    AAATTCCTGT TTGAGAGCGA TTTCTCAGAT GATCCAATGC TTCCCTCACC TGACCAACTT
    AGGAGGAAAG TGCTTCTTAA AAATAAGAAG CTAAAAGCCC ACCAGACACC AGTGGATATC
    TTAAAGCAAA AGGCTCATCA GTTAGCATCC ATGCAAGCTC AGGCTTATAA TGGGGGAAAT
    GTCAACCCTC CACCTGCTAA TAACGAGGAA GAGGAAGATG AAGAGGACGA ATATGATTAT
    GACTATGAAT CCCTGTCTGA TGACAACATT CTAGAAGACA GACCTGAAAA TAAATCATGT
    AATGACAAGC TTCAGTTTGA GTATAACGAA GAAATCCCCA AGAGGATAAA GAAAGCAGAT
    AATTCTGCTT TCAACAAAGG AAAGGTTTAT GACATGGAAC TGGGAGAAGA ATTTTATCTT
    CCTCAGAATA AAAAGGAAAG CAGACAGATT GCACCAGAGC TTTCTGACCT TGTCATCTAC
    TGCCAAGCGG TAAAGTTTCC GGGGCTGTCA ACTCTAAATG CATCTGGCTC TAACAGAGGA
    AAAGAGAGGA AAAGCAGGAA GTCCATTTTT GGCAACAATC CGGGCAGAAT GAGCCCTGGG
    GAGACAGCAT CGTTTAACAA AGCATCTGGA AAAAGTTCCT GTGAAGGAAT GCGACAGGCG
    TGGGAGGAAT CTTCTTCCCC CCTCAACCCA ACCACATCCC TCAGCGCTAT CATTAGAACT
    CCCAAATGTT ACCATATCTC ATCGCTGAAT GAAAATGCCG CCAAACGTCT GTGTCGCAGG
    TATTCTCAGA AACTGATCCA GCACACTGCC TGTCAGCTGC TGAGGACTTA CCCGGCTGCC
    ACACGCATCG ACTCCTCCAA CCCCAATCCT CTCTTGTTCT GGCTCCATGG GATACAGCTC
    GTGGCACTCA ACTACCAGAC AGATGATCTC CCTTTACATT TAAATGCTGC CATGTTTGAG
    GCCAACGGTG GCTGTGGTTA TGTTTTGAAA CCCCCCGTTC TGTGGGACAA GAACTGTCCC
    ATGTACCAGA AGTTTTCACC CTTGGAAAGA GACCTGGACA ACATGGAGCC TGCCGTCTAT
    TCCTTAACTA TTGTCTCTGG TCAAAACGTG TGCCCAAGTA GCAGCACGGG AAGCCCATGT
    ATCGAAGTGG ACGTGCTGGG CATGCCCCTG GACAGTTGCC ACTTCCGCAC AAAACCCATC
    CACCGGAACA CTTTGAACCC CATGTGGAAT GAACAGTTTC TCTTCCGGGT TCACTTTGAA
    GATCTCATAT TCCTGCGTTT TGCAGTTGTG GAAAACAATA GCTCAGCCAT AACCGCTCAG
    AGAATCATCC CCCTCAAGGC TTTAAAACGA GGATATCGAC ATCTTCAGCT GCGAAACCTC
    CACAATGAAG TCTTGGAAAT CTCTAGTTTA TTCATAAACA GCAGAAGGAT GGAAGAAAAT
    TCCTCCAGCA GTGCCACGCC AGCCTCTTTG ATGTTTAATA CAGAAGAAAG AAAATGTTTG
    CAGACTCACA GAGTCACAGT GCATGGGGTC CCAGGTCCAG AGCCCTTTAC TGTTTTCTCC
    ATAAGTGGAG GCACGAAGGC AAAGCAGCTT CTCCAACAGA TTCTAACTAC TGAACAAGAC
    ACCAAACCTA TTGTCACAGA CTATTTTTTG ATGGAAGAAA AATATTTTAT ATCTAAAGAA
    AAGAATGAAT GCAGGAAACA ACCATTTCAG AGAGTCATCG GTCCAGAAGA AGAGATCATG
    CAGATTTTAA GCAGCTGGTT TCCAGAGGAA GGATATGTTG GCAGGATTGT CTTAAAAACC
    CAGCAGGAAA ACCTAGAAGA GAGAAGCATT GTTCAAGATG AGAAAGAGGT GATCTTGAGC
    TCGGAGGAGG AGAGCTTCTT TGTCCAAGTG CATGACGTTT CTCCAGAGCA ACCTCGGACG
    GTCATCAAAG CGCCCCGTGT CAGCACCGCT CAGGATGTCA TTCAACAGAC CTTATGCAAA
    GCCAAATATT CCTATAGCAT CCTGAGTAAC CCCAACCCGA GTGACTATGT GCTTCTGGAA
    GAGGTGGTGA AAGATGCTAC CAAGAAGTCC TCCACCCCAA AGTCCTCCCA GCGGGTCCTT
    TTGGACCAAG AGTGTGTGTT TCAAGCCCAG AGCAAGTGGA AAGGTGCTGG AAAATTCATC
    CTCAGGCTAA AGGAGCAGGT GCAGGCATCC CGAGAAGATA AAAGAAAAGG CATTTCTTTC
    GCAAGTGAAT TCAAGAAGTT CACCAAATCA ACTAAACAGC CCCGAGGACT CACATCTTCT
    CAGGTCTTGG CCTCAGAAAG TGTCCAAAAC AAGGAGGAGA AGCCCACGGG CAGCTTGTCC
    TCCAGTGACA CCACAGACTC CCGACAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001130037.1
    CDS1..6930
    Translation

    Target ORF information:

    RefSeq Version NM_001136565.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris phospholipase C epsilon 1 (PLCE1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001136565.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGACTTCTG AAGGAATGGC AGCTTCTGTT CTCACACCTG TGACTCAAAG AAAAGTGACA 
    TTTGCTCCAT CGGCTGTAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAG TGTTCCAAAG
    GCACATAGTG TCAAGCAGAG TGAGCAGACT TCTACCATCC CGTGGATGAA CAAGCTTAAA
    GAAGAATCTT CTGGAAGCAA CTTGCCCAAA ATTCTCTCCA TAGCAAGGGA GAAAATAGCA
    AGTGATGAGA ACAGCAATGA AGAGTGCTGG ACGGAAAGCA CACCAGTTTC TGTGAAAAAC
    CTTAACATTA ACCATAACAA CATCCTGACA AACCGCCAGT GCGTCCTTCC TCAGAGCCAG
    TCTTATGACA CGTGCAATTC TGTCATGGAG GAAGACCCAT GTTTAGAAAC TGGAATCTCA
    TCCTCACTGG AAAGAAAGGT ATTCCCTGGA ATTCAACTGG AAGTCAACAG ACCTCCCATG
    GACTTTAGGC CTCCAGGCCT AATGGACTTT AGTACCTTAG GAAGTCAGTC AGCCATTGTA
    GATACTGGCC AGGCGCACCC TGACAGCAAC AAAGCAGCAT TTCAAATCTT TAATTATAAA
    GTTGACAGAA GGATGTCTGA CACTTTCTGT ACCCTGTCAG GTGACTTGAT ATTGGATGAC
    TGTGGAAACT GTGTGCCACT GTCATCTGGT TTTGGCGGGG AGCAAAAGAA AAATTACGTG
    GCATATACCT GTAAACTCAT GGAATTGGCT GAAAACTGTG ATAATGAGAA TGGACAGCTG
    CAGTGTGATG ATTATGATGC CTTGGATGAC AAATACTTGT GCTTTGAAGA TTCTTGCCAA
    AGGGACAGTG TGGTATGTTC AAGTGACAGC TTTCGCAGGG AGGACCTAAC TAACAGTCCA
    CCTGCCAAGA CCTTTCTGAG CCATTTTGAA GACTTCCCTG ATAATGGTGA AGATGTAGAA
    GATTTTTTAA AAAACAAAAA GGAGAGATCC ACTTTGTTAG TGAGGAGATT TTGTAAAAAT
    GACAGAGAAG TAAAGAAATC TGTGTATACT GGGACAAGGG CAATCATGAG AACTCTGCCT
    TCTGGTCACA TTGGGCTGGA AGCTTACAGT TACATCGATC AGAAGAGAAG TGGTCCCTTA
    TTACCCCGTG GGAGAGTTCT GGAACAGCTG CCAGTGGTGG CAATTAGGCA AGATGGGAGC
    CAGTGCCTGT CAGAAGCCCA GTGGTATCGG ATCTACAATG CAGTGAGAAG GGAAGAAGAA
    ATAGAAAATA CCATTGGATC TCTTCTCCAT TACTTTACCA AGCTCCCAGC CTCCAAGACA
    GCCCATGAAA GGATTAGTGT TGGTCCATGC CTAAAGCAGT GTGTCCGAGA CACCATATGT
    GAGTATCGTG CCACCCTCCA GAGGACGTCC ATATCTCAGT ACATCACGGG TTCGCTCCTA
    GAAGCAACCA CATCTTTAGG AGCGAGAAGT GGCCTACTCA GTACTTTTGG AGGATCCACT
    GGACGAATGA TGCTAAAAGA ACGCCAGCCA GGCACCTCCA TGGCCAATTC CAGTGCCCTC
    CCTTCAAGTT CCGCTGGAAT CAGCAAGGAG CTGATTGACC TGCAGCCTCT CATTCAGTTC
    CCAGAGGAAG TCGCCAGCAT CCTGATGGAG CAGGAACAGA ACATTTACCG AAGGGTCTTA
    CCAGTTGACT ATCTTTACTT CTTGACCCGG GACTTGGGCA CTCCAGAATG CCAGACACCC
    CTGCCCTGCC TCAAAGCATC CATCTCAGCT TCAATTCTTA GCTCCCAGAA CGGAGAGCAC
    AATGCCCTGG AAGATCTTGT GATGAGATTT AATGAGGTGA GCTCCTGGGT GACATGGCTG
    ATCCTCACGG CGGGCTCCAT GGAGGAGAAG CGAGAAGTCT TCTCCTATTT GGTGCATGTG
    GCCAAATGCT GCTGGAACAT GGGCAACTAC AATGCTGTCA TGGAGTTCTT GGCTGGTCTC
    AGGTCAAGAA AAGTTTTAAA AATGTGGCAG TTTATGGACC AATCTGACCT CGAGACCATG
    AGGAGCCTGA AGGACGCCAT GGCTCAGCAT GAATCTTCCT GCGAATATCG GAAGGTGATA
    ACGAGGGCTC TGCACATACC TGGCTGTAAA GTGGTTCCAT TCTGTGGAGT GTTTCTGAAG
    GAGCTCTGTG AAGTACTGGA CGGAGCCTCT GGCCTCATGA AGCTTTGCCC GAGGTACAAT
    TCCCAAGAAG AAACTCTAGA GTTTGTAGCA GATTACAGTG GACAAGATAA TTTCTTACAA
    CGAGTGGGGC ATAATGGCTT AAAGAATTCA GAGAAGGAGT CCACTGTCAA CAGCATCTTT
    CAAATCATTA GGAGCTGCAG CCGAAGTCTG GAGGCTGAGG AAGAGGACAG CCCCAGTGAA
    GGGAACAGCT CTAGGAAAAA TTCTTTAAGG GACAAAGCCC GATGGCAATT TATAATCGGA
    GATTTGTTGG ATTCCGAAAA TGACATCTTT GAGCAATCCA AAGAATGGGA CTCTCCCAGC
    TCAGAAGAGC CTCAGAAAGC CTTTGACCAT GGGACAGAGC TGATCCCTTG GTACGTGCTG
    TCCATCCGAG CAGATGTGCA CCAGTTCCTG CTACAGGGCG CCACCGTCCT CCGCTACGAC
    CAGGACACGC ACCTCTCTGC CCGCTGCTTC CTCCAGCTTC AGCCTGACAA TAGCACCTTG
    ACATGGATAA AGCCCACAGC TGCCTCCCCG GCCAGTGCTA GAGCAAAACT CGGTGTGCTG
    AGCAACACGT CTGAGCCTGG CAAGTTCCCG TCACCAGGCA GTGCCGGACT AAGCGGCCTG
    GCCGAGGGGG TCCTGGATCT GTTTTCAGCA AAGGCTGTGT ACATGGGACA CCCTAGCATT
    GATATCCACA CTGTGTGTGT CCAGAACAAA CTCGGTAGCA TGTTTCTCTC AGAGACTGGC
    GTGACCCTGC TCTACGGGCT CCAGACCACA GACAATAGAT TATTGCACTT TGTGGCACCC
    AAACACACTG CTAAGATGCT CTTCAGTGGA TTGCTAGAAC TCACGAGGGC CGTGAGGAAG
    ATGAGAAAGT TCCCTGACCA GAGGCAGCAG TGGCTGCGGA AACAGTATGT TAGCCTCTAT
    CAGGAGGACG GACGGTATGA GGGCCCAACT TTGGCTCATG CTGTGGAGTT GTTTGGTGGC
    CGGCGATGGA GTACACGCAA TCCCAGCCCT GGGACATCTG CAAAGAGTGC TGAGAAGCCC
    AGTGTGCAGA GAAACAACAC CTTGGGCATA AGCACCACCA AGAAAAAGAA GAAAATCCTT
    ATAAGGGGTG AGAGTGGAGA GGCAGCCGAC GATGAGATGG CCACCCGGAA GGCCAAAATG
    CACAGGGAGT GTCGCAGCCG GAGTGGTTCT GACCCACAAG ACATGAATGA GCAAGAAGAA
    TCTGAGGCAA ATGCCATCAT GAGCCCTCCT AACACTCTGC CTTCCAGAAG AGCCCACTCT
    TTGACTACAG CTGGGTCCCC TAATTTGTCT GCCGGGACAT CATCTCCCAT CAGGCCAGTG
    TCCTCCCCTG TGCTGTCTTC TTCAAACAAG AGCCCGTCCA GTGCTTGGAG CAGCAGCAGC
    TGGCATGGAA GGATCAAAGG AGGAATGAAG GGTTTTCAGA GCTTCATGGT TTCAGACAGT
    AACATGAGTT TTGTTGAATT TGTTGAGCTG TTCAAATCAT TCAGTGTGAG GAGCCGCAAG
    GACCTGAAGG ATCTCTTTGA CATCTACGCT GTGCCCTGCA ATCGGGCCGG CTCTGAGTCG
    GCCCCGCTCT ACACCAACCT AACAATAGAC GAAAACACCA GCGATCTTCA GCCTGACCTA
    GATTTATTGA CCAGAAACGT CTCGGATTTG GGGTTGTTCA TTAAGAGTAA ACAGCAGCTG
    TCAGACAACC AGAGGCAGAT TTCTGATGCC ATTGCTGCTG CAAGTATCGT GACGAATGGC
    ACTGGGATCG AGAGCACATC TTTGGGCATC TTTGGGGTTG GCATACTCCA GCTCAATGAC
    TTCCTAGTCA ACTGCCAGGG AGAACACTGC ACTTACGATG AAATCCTCAG CATCATCCAG
    AAGTTCGAGC CTAGCATTAG TATGTGTCAT CAGGGCCTAC TGTCCTTTGA AGGATTTGCA
    AGGTTTCTGA TGGATAAAGA TAATTTTGCC TCCAAAAATG ATGAGTCACA GGAGAACATT
    AAAGATTTGC AGCTGCCTCT CTCCTACTAT TACATTGAGT CTTCACACAA TACCTACCTC
    ACAGGCCACC AGCTCAAAGG AGAGTCATCG GTAGAACTCT ATAGCCAGGT CCTCCTGCAA
    GGCTGTAGAA GTGTAGAGTT GGACTGCTGG GATGGAGATG ACGGGATGCC CATCATCTAT
    CACGGACACA CACTAACAAC CAAGATCCCC TTCAAGGAGG TGGTTGAAGC CATCGACCGC
    AGTGCCTTCA TCAACTCTGA CCTGCCCATC ATCATATCGA TTGAGAACCA CTGCTCGTTG
    CCTCAGCAAC GAAAAATGGC AGAAATTTTC AAGACTGTGT TTGGAGAAAA ACTGGTGGCT
    AAATTCCTGT TTGAGAGCGA TTTCTCAGAT GATCCAATGC TTCCCTCACC TGACCAACTT
    AGGAGGAAAG TGCTTCTTAA AAATAAGAAG CTAAAAGCCC ACCAGACACC AGTGGATATC
    TTAAAGCAAA AGGCTCATCA GTTAGCATCC ATGCAAGCTC AGGCTTATAA TGGGGGAAAT
    GTCAACCCTC CACCTGCTAA TAACGAGGAA GAGGAAGATG AAGAGGACGA ATATGATTAT
    GACTATGAAT CCCTGTCTGA TGACAACATT CTAGAAGACA GACCTGAAAA TAAATCATGT
    AATGACAAGC TTCAGTTTGA GTATAACGAA GAAATCCCCA AGAGGATAAA GAAAGCAGAT
    AATTCTGCTT TCAACAAAGG AAAGGTTTAT GACATGGAAC TGGGAGAAGA ATTTTATCTT
    CCTCAGAATA AAAAGGAAAG CAGACAGATT GCACCAGAGC TTTCTGACCT TGTCATCTAC
    TGCCAAGCGG TAAAGTTTCC GGGGCTGTCA ACTCTAAATG CATCTGGCTC TAACAGAGGA
    AAAGAGAGGA AAAGCAGGAA GTCCATTTTT GGCAACAATC CGGGCAGAAT GAGCCCTGGG
    GAGACAGCAT CGTTTAACAA AGCATCTGGA AAAAGTTCCT GTGAAGGAAT GCGACAGGCG
    TGGGAGGAAT CTTCTTCCCC CCTCAACCCA ACCACATCCC TCAGCGCTAT CATTAGAACT
    CCCAAATGTT ACCATATCTC ATCGCTGAAT GAAAATGCCG CCAAACGTCT GTGTCGCAGG
    TATTCTCAGA AACTGATCCA GCACACTGCC TGTCAGCTGC TGAGGACTTA CCCGGCTGCC
    ACACGCATCG ACTCCTCCAA CCCCAATCCT CTCTTGTTCT GGCTCCATGG GATACAGCTC
    GTGGCACTCA ACTACCAGAC AGATGATCTC CCTTTACATT TAAATGCTGC CATGTTTGAG
    GCCAACGGTG GCTGTGGTTA TGTTTTGAAA CCCCCCGTTC TGTGGGACAA GAACTGTCCC
    ATGTACCAGA AGTTTTCACC CTTGGAAAGA GACCTGGACA ACATGGAGCC TGCCGTCTAT
    TCCTTAACTA TTGTCTCTGG TCAAAACGTG TGCCCAAGTA GCAGCACGGG AAGCCCATGT
    ATCGAAGTGG ACGTGCTGGG CATGCCCCTG GACAGTTGCC ACTTCCGCAC AAAACCCATC
    CACCGGAACA CTTTGAACCC CATGTGGAAT GAACAGTTTC TCTTCCGGGT TCACTTTGAA
    GATCTCATAT TCCTGCGTTT TGCAGTTGTG GAAAACAATA GCTCAGCCAT AACCGCTCAG
    AGAATCATCC CCCTCAAGGC TTTAAAACGA GGATATCGAC ATCTTCAGCT GCGAAACCTC
    CACAATGAAG TCTTGGAAAT CTCTAGTTTA TTCATAAACA GCAGAAGGAT GGAAGAAAAT
    TCCTCCAGCA GTGCCACGCC AGCCTCTTTG ATGTTTAATA CAGAAGAAAG AAAATGTTTG
    CAGACTCACA GAGTCACAGT GCATGGGGTC CCAGGTCCAG AGCCCTTTAC TGTTTTCTCC
    ATAAGTGGAG GCACGAAGGC AAAGCAGCTT CTCCAACAGA TTCTAACTAC TGAACAAGAC
    ACCAAACCTA TTGTCACAGA CTATTTTTTG ATGGAAGAAA AATATTTTAT ATCTAAAGAA
    AAGAATGAAT GCAGGAAACA ACCATTTCAG AGAGTCATCG GTCCAGAAGA AGAGATCATG
    CAGATTTTAA GCAGCTGGTT TCCAGAGGAA GGATATGTTG GCAGGATTGT CTTAAAAACC
    CAGCAGGAAA ACCTAGAAGA GAGAAGCATT GTTCAAGATG AGAAAGAGGT GATCTTGAGC
    TCGGAGGAGG AGAGCTTCTT TGTCCAAGTG CATGACGTTT CTCCAGAGCA ACCTCGGACG
    GTCATCAAAG CGCCCCGTGT CAGCACCGCT CAGGATGTCA TTCAACAGAC CTTATGCAAA
    GCCAAATATT CCTATAGCAT CCTGAGTAAC CCCAACCCGA GTGACTATGT GCTTCTGGAA
    GAGGTGGTGA AAGATGCTAC CAAGAAGTCC TCCACCCCAA AGTCCTCCCA GCGGGTCCTT
    TTGGACCAAG AGTGTGTGTT TCAAGCCCAG AGCAAGTGGA AAGGTGCTGG AAAATTCATC
    CTCAGGCTAA AGGAGCAGGT GCAGGCATCC CGAGAAGATA AAAGAAAAGG CATTTCTTTC
    GCAAGTGAAT TCAAGAAGTT CACCAAATCA ACTAAACAGC CCCGAGGACT CACATCTTCT
    CAGGTCTTGG CCTCAGAAAG TGTCCAAAAC AAGGAGGAGA AGCCCACGGG CAGCTTGTCC
    TCCAGTGACA CCACAGACTC CCGACAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27695
    Clone ID Related Accession (Same CDS sequence) XM_022411584.1 , XM_005637458.2 , XM_022411585.1
    Accession Version XM_022411584.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6972bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006610.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGACTTCTG AAGGAATGGC AGCTTCTGTT CTCACACCTG TGACTCAAAG AAAAGTGACA 
    TTTGCTCCAT CGGCTGTAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAG TGTTCCAAAG
    GCACATAGTG TCAAGCAGAG TGAGCAGACT TCTACCATCC CGTGGATGAA CAAGCTTAAA
    GAAGAATCTT CTGGAAGCAA CTTGCCCAAA ATTCTCTCCA TAGCAAGGGA GAAAATAGCA
    AGTGATGAGA ACAGCAATGA AGAGTGCTGG ACGGAAAGCA CACCAGTTTC TGTGAAAAAC
    CTTAACATTA ACCATAACAA CATCCTGACA AACCGCCAGT GCGTCCTTCC TCAGAGCCAG
    TCTTATGACA CGTGCAATTC TGTCATGGAG GAAGACCCAT GTTTAGAAAC TGGAATCTCA
    TCCTCACTGG AAAGAAAGGT ATTCCCTGGA ATTCAACTGG AAGTCAACAG ACCTCCCATG
    GACTTTAGGC CTCCAGGCCT AATGGACTTT AGTACCTTAG GAAGTCAGTC AGCCATTGTA
    GATACTGGCC AGGCGCACCC TGACAGCAAC AAAGCAGCAT TTCAAATCTT TAATTATAAA
    GTTGACAGAA GGATGTCTGA CACTTTCTGT ACCCTGTCAG GTGACTTGAT ATTGGATGAC
    TGTGGAAACT GTGTGCCACT GTCATCTGGT TTTGGCGGGG AGCAAAAGAA AAATTACGTG
    GCATATACCT GTAAACTCAT GGAATTGGCT GAAAACTGTG ATAATGAGAA TGGACAGCTG
    CAGTGTGATG ATTATGATGC CTTGGATGAC AAATACTTGT GCTTTGAAGA TTCTTGCCAA
    AGGGACAGTG TGGTATGTTC AAGTGACAGC TTTCGCAGGG AGGACCTAAC TAACAGTCCA
    CCTGCCAAGA CCTTTCTGAG CCATTTTGAA GACTTCCCTG ATAATGGTGA AGATGTAGAA
    GATTTTTTAA AAAACAAAAA GGAGAGATCC ACTTTGTTAG TGAGGAGATT TTGTAAAAAT
    GACAGAGAAG TAAAGAAATC TGTGTATACT GGGACAAGGG CAATCATGAG AACTCTGCCT
    TCTGGTCACA TTGGGCTGGA AGCTTACAGT TACATCGATC AGAAGAGAAG TGGTCCCTTA
    TTACCCCGTG GGAGAGTTCT GGAACAGCTG CCAGTGGTGG CAATTAGGCA AGATGGGAGC
    CAGTGCCTGT CAGAAGCCCA GTGGTATCGG ATCTACAATG CAGTGAGAAG GGAAGAAGAA
    ATAGAAAATA CCATTGGATC TCTTCTCCAT TACTTTACCA AGCTCCCAGC CTCCAAGACA
    GCCCATGAAA GGATTAGTGT TGGTCCATGC CTAAAGCAGT GTGTCCGAGA CACCATATGT
    GAGTATCGTG CCACCCTCCA GAGGACGTCC ATATCTCAGT ACATCACGGG TTCGCTCCTA
    GAAGCAACCA CATCTTTAGG AGCGAGAAGT GGCCTACTCA GTACTTTTGG AGGATCCACT
    GGACGAATGA TGCTAAAAGA ACGCCAGCCA GGCACCTCCA TGGCCAATTC CAGTGCCCTC
    CCTTCAAGTT CCGCTGGAAT CAGCAAGGAG CTGATTGACC TGCAGCCTCT CATTCAGTTC
    CCAGAGGAAG TCGCCAGCAT CCTGATGGAG CAGGAACAGA ACATTTACCG AAGGGTCTTA
    CCAGTTGACT ATCTTTACTT CTTGACCCGG GACTTGGGCA CTCCAGAATG CCAGACACCC
    CTGCCCTGCC TCAAAGCATC CATCTCAGCT TCAATTCTTA GCTCCCAGAA CGGAGAGCAC
    AATGCCCTGG AAGATCTTGT GATGAGATTT AATGAGGTGA GCTCCTGGGT GACATGGCTG
    ATCCTCACGG CGGGCTCCAT GGAGGAGAAG CGAGAAGTCT TCTCCTATTT GGTGCATGTG
    GCCAAATGCT GCTGGAACAT GGGCAACTAC AATGCTGTCA TGGAGTTCTT GGCTGGTCTC
    AGGTCAAGAA AAGTTTTAAA AATGTGGCAG TTTATGGACC AATCTGACCT CGAGACCATG
    AGGAGCCTGA AGGACGCCAT GGCTCAGCAT GAATCTTCCT GCGAATATCG GAAGGTGATA
    ACGAGGGCTC TGCACATACC TGGCTGTAAA GTGGTTCCAT TCTGTGGAGT GTTTCTGAAG
    GAGCTCTGTG AAGTACTGGA CGGAGCCTCT GGCCTCATGA AGCTTTGCCC GAGGTACAAT
    TCCCAAGAAG AAACTCTAGA GTTTGTAGCA GATTACAGTG GACAAGATAA TTTCTTACAA
    CGAGTGGGGC ATAATGGCTT AAAGAATTCA GAGAAGGAGT CCACTGTCAA CAGCATCTTT
    CAAATCATTA GGAGCTGCAG CCGAAGTCTG GAGGCTGAGG AAGAGGACAG CCCCAGTGAA
    GGGAACAGCT CTAGGAAAAA TTCTTTAAGG GACAAAGCCC GATGGCAATT TATAATCGGA
    GATTTGTTGG ATTCCGAAAA TGACATCTTT GAGCAATCCA AAGAATGGGA CTCTCCCAGC
    TCAGAAGAGC CTCAGAAAGC CTTTGACCAT GGGACAGAGC TGATCCCTTG GTACGTGCTG
    TCCATCCGAG CAGATGTGCA CCAGTTCCTG CTACAGGGCG CCACCGTCCT CCGCTACGAC
    CAGGACACGC ACCTCTCTGC CCGCTGCTTC CTCCAGCTTC AGCCTGACAA TAGCACCTTG
    ACATGGATAA AGCCCACAGC TGCCTCCCCG GCCAGTGCTA GAGCAAAACT CGGTGTGCTG
    AGCAACACGT CTGAGCCTGG CAAGTTCCCG TCACCAGGCA GTGCCGGACT AAGCGGCCTG
    GCCGAGGGGG TCCTGGATCT GTTTTCAGCA AAGGCTGTGT ACATGGGACA CCCTAGCATT
    GATATCCACA CTGTGTGTGT CCAGAACAAA CTCGGTAGCA TGTTTCTCTC AGAGACTGGC
    GTGACCCTGC TCTACGGGCT CCAGACCACA GACAATAGAT TATTGCACTT TGTGGCACCC
    AAACACACTG CTAAGATGCT CTTCAGTGGA TTGCTAGAAC TCACGAGGGC CGTGAGGAAG
    ATGAGAAAGT TCCCTGACCA GAGGCAGCAG TGGCTGCGGA AACAGTATGT TAGCCTCTAT
    CAGGAGGACG GACGGTATGA GGGCCCAACT TTGGCTCATG CTGTGGAGTT GTTTGGTGGC
    CGGCGATGGA GTACACGCAA TCCCAGCCCT GGGACATCTG CAAAGAGTGC TGAGAAGCCC
    AGTGTGCAGA GAAACAACAC CTTGGGCATA AGCACCACCA AGAAAAAGAA GAAAATCCTT
    ATAAGGGGTG AGAGTGGAGA GGCAGCCGAC GATGAGATGG CCACCCGGAA GGCCAAAATG
    CACAGGGAGT GTCGCAGCCG GAGTGGTTCT GACCCACAAG ACATGAATGA GCAAGAAGAA
    TCTGAGGCAA ATGCCATCAT GAGCCCTCCT AACACTCTGC CTTCCAGAAG AGCCCACTCT
    TTGACTACAG CTGGGTCCCC TAATTTGTCT GCCGGGACAT CATCTCCCAT CAGGCCAGTG
    TCCTCCCCTG TGCTGTCTTC TTCAAACAAG AGCCCGTCCA GTGCTTGGAG CAGCAGCAGC
    TGGCATGGAA GGATCAAAGG AGGAATGAAG GGTTTTCAGA GCTTCATGGT TTCAGACAGT
    AACATGAGTT TTGTTGAATT TGTTGAGCTG TTCAAATCAT TCAGTGTGAG GAGCCGCAAG
    GACCTGAAGG ATCTCTTTGA CATCTACGCT GTGCCCTGCA ATCGGGCCGG CTCTGAGTCG
    GCCCCGCTCT ACACCAACCT AACAATAGAC GAAAACACCA GCGATCTTCA GCCTGACCTA
    GATTTATTGA CCAGAAACGT CTCGGATTTG GGGTTGTTCA TTAAGAGTAA ACAGCAGCTG
    TCAGACAACC AGAGGCAGAT TTCTGATGCC ATTGCTGCTG CAAGTATCGT GACGAATGGC
    ACTGGGATCG AGAGCACATC TTTGGGCATC TTTGGGGTTG GCATACTCCA GCTCAATGAC
    TTCCTAGTCA ACTGCCAGGG AGAACACTGC ACTTACGATG AAATCCTCAG CATCATCCAG
    AAGTTCGAGC CTAGCATTAG TATGTGTCAT CAGGGCCTAC TGTCCTTTGA AGGATTTGCA
    AGGTTTCTGA TGGATAAAGA TAATTTTGCC TCCAAAAATG ATGAGTCACA GGAGAACATT
    AAAGATTTGC AGCTGCCTCT CTCCTACTAT TACATTGAGT CTTCACACAA TACCTACCTC
    ACAGGCCACC AGCTCAAAGG AGAGTCATCG GTAGAACTCT ATAGCCAGGT CCTCCTGCAA
    GGCTGTAGAA GTGTAGAGTT GGACTGCTGG GATGGAGATG ACGGGATGCC CATCATCTAT
    CACGGACACA CACTAACAAC CAAGATCCCC TTCAAGGAGG TGGTTGAAGC CATCGACCGC
    AGTGCCTTCA TCAACTCTGA CCTGCCCATC ATCATATCGA TTGAGAACCA CTGCTCGTTG
    CCTCAGCAAC GAAAAATGGC AGAAATTTTC AAGACTGTGT TTGGAGAAAA ACTGGTGGCT
    AAATTCCTGT TTGAGAGCGA TTTCTCAGAT GATCCAATGC TTCCCTCACC TGACCAACTT
    AGGAGGAAAG TGCTTCTTAA AAATAAGAAG CTAAAAGCCC ACCAGACACC AGTGGATATC
    TTAAAGCAAA AGGCTCATCA GTTAGCATCC ATGCAAGCTC AGGCTTATAA TGGGGGAAAT
    GTCAACCCTC CACCTGCTAA TAACGAGGAA GAGGAAGATG AAGAGGACGA ATATGATTAT
    GACTATGAAT CCCTGTCTGA TGCAGATGTC CTTACTGCTT CTCCTGCTCC TAACAGTCAG
    GAAGACAACA TTCTAGAAGA CAGACCTGAA AATAAATCAT GTAATGACAA GCTTCAGTTT
    GAGTATAACG AAGAAATCCC CAAGAGGATA AAGAAAGCAG ATAATTCTGC TTTCAACAAA
    GGAAAGGTTT ATGACATGGA ACTGGGAGAA GAATTTTATC TTCCTCAGAA TAAAAAGGAA
    AGCAGACAGA TTGCACCAGA GCTTTCTGAC CTTGTCATCT ACTGCCAAGC GGTAAAGTTT
    CCGGGGCTGT CAACTCTAAA TGCATCTGGC TCTAACAGAG GAAAAGAGAG GAAAAGCAGG
    AAGTCCATTT TTGGCAACAA TCCGGGCAGA ATGAGCCCTG GGGAGACAGC ATCGTTTAAC
    AAAGCATCTG GAAAAAGTTC CTGTGAAGGA ATGCGACAGG CGTGGGAGGA ATCTTCTTCC
    CCCCTCAACC CAACCACATC CCTCAGCGCT ATCATTAGAA CTCCCAAATG TTACCATATC
    TCATCGCTGA ATGAAAATGC CGCCAAACGT CTGTGTCGCA GGTATTCTCA GAAACTGATC
    CAGCACACTG CCTGTCAGCT GCTGAGGACT TACCCGGCTG CCACACGCAT CGACTCCTCC
    AACCCCAATC CTCTCTTGTT CTGGCTCCAT GGGATACAGC TCGTGGCACT CAACTACCAG
    ACAGATGATC TCCCTTTACA TTTAAATGCT GCCATGTTTG AGGCCAACGG TGGCTGTGGT
    TATGTTTTGA AACCCCCCGT TCTGTGGGAC AAGAACTGTC CCATGTACCA GAAGTTTTCA
    CCCTTGGAAA GAGACCTGGA CAACATGGAG CCTGCCGTCT ATTCCTTAAC TATTGTCTCT
    GGTCAAAACG TGTGCCCAAG TAGCAGCACG GGAAGCCCAT GTATCGAAGT GGACGTGCTG
    GGCATGCCCC TGGACAGTTG CCACTTCCGC ACAAAACCCA TCCACCGGAA CACTTTGAAC
    CCCATGTGGA ATGAACAGTT TCTCTTCCGG GTTCACTTTG AAGATCTCAT ATTCCTGCGT
    TTTGCAGTTG TGGAAAACAA TAGCTCAGCC ATAACCGCTC AGAGAATCAT CCCCCTCAAG
    GCTTTAAAAC GAGGATATCG ACATCTTCAG CTGCGAAACC TCCACAATGA AGTCTTGGAA
    ATCTCTAGTT TATTCATAAA CAGCAGAAGG ATGGAAGAAA ATTCCTCCAG CAGTGCCACG
    CCAGCCTCTT TGATGTTTAA TACAGAAGAA AGAAAATGTT TGCAGACTCA CAGAGTCACA
    GTGCATGGGG TCCCAGGTCC AGAGCCCTTT ACTGTTTTCT CCATAAGTGG AGGCACGAAG
    GCAAAGCAGC TTCTCCAACA GATTCTAACT ACTGAACAAG ACACCAAACC TATTGTCACA
    GACTATTTTT TGATGGAAGA AAAATATTTT ATATCTAAAG AAAAGAATGA ATGCAGGAAA
    CAACCATTTC AGAGAGTCAT CGGTCCAGAA GAAGAGATCA TGCAGATTTT AAGCAGCTGG
    TTTCCAGAGG AAGGATATGT TGGCAGGATT GTCTTAAAAA CCCAGCAGGA AAACCTAGAA
    GAGAGAAGCA TTGTTCAAGA TGAGAAAGAG GTGATCTTGA GCTCGGAGGA GGAGAGCTTC
    TTTGTCCAAG TGCATGACGT TTCTCCAGAG CAACCTCGGA CGGTCATCAA AGCGCCCCGT
    GTCAGCACCG CTCAGGATGT CATTCAACAG ACCTTATGCA AAGCCAAATA TTCCTATAGC
    ATCCTGAGTA ACCCCAACCC GAGTGACTAT GTGCTTCTGG AAGAGGTGGT GAAAGATGCT
    ACCAAGAAGT CCTCCACCCC AAAGTCCTCC CAGCGGGTCC TTTTGGACCA AGAGTGTGTG
    TTTCAAGCCC AGAGCAAGTG GAAAGGTGCT GGAAAATTCA TCCTCAGGCT AAAGGAGCAG
    GTGCAGGCAT CCCGAGAAGA TAAAAGAAAA GGCATTTCTT TCGCAAGTGA ATTCAAGAAG
    TTCACCAAAT CAACTAAACA GCCCCGAGGA CTCACATCTT CTCAGGTCTT GGCCTCAGAA
    AGTGTCCAAA ACAAGGAGGA GAAGCCCACG GGCAGCTTGT CCTCCAGTGA CACCACAGAC
    TCCCGACAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022267292.1
    CDS352..7323
    Translation

    Target ORF information:

    RefSeq Version XM_022411584.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris phospholipase C epsilon 1 (PLCE1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022411584.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGACTTCTG AAGGAATGGC AGCTTCTGTT CTCACACCTG TGACTCAAAG AAAAGTGACA 
    TTTGCTCCAT CGGCTGTAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAG TGTTCCAAAG
    GCACATAGTG TCAAGCAGAG TGAGCAGACT TCTACCATCC CGTGGATGAA CAAGCTTAAA
    GAAGAATCTT CTGGAAGCAA CTTGCCCAAA ATTCTCTCCA TAGCAAGGGA GAAAATAGCA
    AGTGATGAGA ACAGCAATGA AGAGTGCTGG ACGGAAAGCA CACCAGTTTC TGTGAAAAAC
    CTTAACATTA ACCATAACAA CATCCTGACA AACCGCCAGT GCGTCCTTCC TCAGAGCCAG
    TCTTATGACA CGTGCAATTC TGTCATGGAG GAAGACCCAT GTTTAGAAAC TGGAATCTCA
    TCCTCACTGG AAAGAAAGGT ATTCCCTGGA ATTCAACTGG AAGTCAACAG ACCTCCCATG
    GACTTTAGGC CTCCAGGCCT AATGGACTTT AGTACCTTAG GAAGTCAGTC AGCCATTGTA
    GATACTGGCC AGGCGCACCC TGACAGCAAC AAAGCAGCAT TTCAAATCTT TAATTATAAA
    GTTGACAGAA GGATGTCTGA CACTTTCTGT ACCCTGTCAG GTGACTTGAT ATTGGATGAC
    TGTGGAAACT GTGTGCCACT GTCATCTGGT TTTGGCGGGG AGCAAAAGAA AAATTACGTG
    GCATATACCT GTAAACTCAT GGAATTGGCT GAAAACTGTG ATAATGAGAA TGGACAGCTG
    CAGTGTGATG ATTATGATGC CTTGGATGAC AAATACTTGT GCTTTGAAGA TTCTTGCCAA
    AGGGACAGTG TGGTATGTTC AAGTGACAGC TTTCGCAGGG AGGACCTAAC TAACAGTCCA
    CCTGCCAAGA CCTTTCTGAG CCATTTTGAA GACTTCCCTG ATAATGGTGA AGATGTAGAA
    GATTTTTTAA AAAACAAAAA GGAGAGATCC ACTTTGTTAG TGAGGAGATT TTGTAAAAAT
    GACAGAGAAG TAAAGAAATC TGTGTATACT GGGACAAGGG CAATCATGAG AACTCTGCCT
    TCTGGTCACA TTGGGCTGGA AGCTTACAGT TACATCGATC AGAAGAGAAG TGGTCCCTTA
    TTACCCCGTG GGAGAGTTCT GGAACAGCTG CCAGTGGTGG CAATTAGGCA AGATGGGAGC
    CAGTGCCTGT CAGAAGCCCA GTGGTATCGG ATCTACAATG CAGTGAGAAG GGAAGAAGAA
    ATAGAAAATA CCATTGGATC TCTTCTCCAT TACTTTACCA AGCTCCCAGC CTCCAAGACA
    GCCCATGAAA GGATTAGTGT TGGTCCATGC CTAAAGCAGT GTGTCCGAGA CACCATATGT
    GAGTATCGTG CCACCCTCCA GAGGACGTCC ATATCTCAGT ACATCACGGG TTCGCTCCTA
    GAAGCAACCA CATCTTTAGG AGCGAGAAGT GGCCTACTCA GTACTTTTGG AGGATCCACT
    GGACGAATGA TGCTAAAAGA ACGCCAGCCA GGCACCTCCA TGGCCAATTC CAGTGCCCTC
    CCTTCAAGTT CCGCTGGAAT CAGCAAGGAG CTGATTGACC TGCAGCCTCT CATTCAGTTC
    CCAGAGGAAG TCGCCAGCAT CCTGATGGAG CAGGAACAGA ACATTTACCG AAGGGTCTTA
    CCAGTTGACT ATCTTTACTT CTTGACCCGG GACTTGGGCA CTCCAGAATG CCAGACACCC
    CTGCCCTGCC TCAAAGCATC CATCTCAGCT TCAATTCTTA GCTCCCAGAA CGGAGAGCAC
    AATGCCCTGG AAGATCTTGT GATGAGATTT AATGAGGTGA GCTCCTGGGT GACATGGCTG
    ATCCTCACGG CGGGCTCCAT GGAGGAGAAG CGAGAAGTCT TCTCCTATTT GGTGCATGTG
    GCCAAATGCT GCTGGAACAT GGGCAACTAC AATGCTGTCA TGGAGTTCTT GGCTGGTCTC
    AGGTCAAGAA AAGTTTTAAA AATGTGGCAG TTTATGGACC AATCTGACCT CGAGACCATG
    AGGAGCCTGA AGGACGCCAT GGCTCAGCAT GAATCTTCCT GCGAATATCG GAAGGTGATA
    ACGAGGGCTC TGCACATACC TGGCTGTAAA GTGGTTCCAT TCTGTGGAGT GTTTCTGAAG
    GAGCTCTGTG AAGTACTGGA CGGAGCCTCT GGCCTCATGA AGCTTTGCCC GAGGTACAAT
    TCCCAAGAAG AAACTCTAGA GTTTGTAGCA GATTACAGTG GACAAGATAA TTTCTTACAA
    CGAGTGGGGC ATAATGGCTT AAAGAATTCA GAGAAGGAGT CCACTGTCAA CAGCATCTTT
    CAAATCATTA GGAGCTGCAG CCGAAGTCTG GAGGCTGAGG AAGAGGACAG CCCCAGTGAA
    GGGAACAGCT CTAGGAAAAA TTCTTTAAGG GACAAAGCCC GATGGCAATT TATAATCGGA
    GATTTGTTGG ATTCCGAAAA TGACATCTTT GAGCAATCCA AAGAATGGGA CTCTCCCAGC
    TCAGAAGAGC CTCAGAAAGC CTTTGACCAT GGGACAGAGC TGATCCCTTG GTACGTGCTG
    TCCATCCGAG CAGATGTGCA CCAGTTCCTG CTACAGGGCG CCACCGTCCT CCGCTACGAC
    CAGGACACGC ACCTCTCTGC CCGCTGCTTC CTCCAGCTTC AGCCTGACAA TAGCACCTTG
    ACATGGATAA AGCCCACAGC TGCCTCCCCG GCCAGTGCTA GAGCAAAACT CGGTGTGCTG
    AGCAACACGT CTGAGCCTGG CAAGTTCCCG TCACCAGGCA GTGCCGGACT AAGCGGCCTG
    GCCGAGGGGG TCCTGGATCT GTTTTCAGCA AAGGCTGTGT ACATGGGACA CCCTAGCATT
    GATATCCACA CTGTGTGTGT CCAGAACAAA CTCGGTAGCA TGTTTCTCTC AGAGACTGGC
    GTGACCCTGC TCTACGGGCT CCAGACCACA GACAATAGAT TATTGCACTT TGTGGCACCC
    AAACACACTG CTAAGATGCT CTTCAGTGGA TTGCTAGAAC TCACGAGGGC CGTGAGGAAG
    ATGAGAAAGT TCCCTGACCA GAGGCAGCAG TGGCTGCGGA AACAGTATGT TAGCCTCTAT
    CAGGAGGACG GACGGTATGA GGGCCCAACT TTGGCTCATG CTGTGGAGTT GTTTGGTGGC
    CGGCGATGGA GTACACGCAA TCCCAGCCCT GGGACATCTG CAAAGAGTGC TGAGAAGCCC
    AGTGTGCAGA GAAACAACAC CTTGGGCATA AGCACCACCA AGAAAAAGAA GAAAATCCTT
    ATAAGGGGTG AGAGTGGAGA GGCAGCCGAC GATGAGATGG CCACCCGGAA GGCCAAAATG
    CACAGGGAGT GTCGCAGCCG GAGTGGTTCT GACCCACAAG ACATGAATGA GCAAGAAGAA
    TCTGAGGCAA ATGCCATCAT GAGCCCTCCT AACACTCTGC CTTCCAGAAG AGCCCACTCT
    TTGACTACAG CTGGGTCCCC TAATTTGTCT GCCGGGACAT CATCTCCCAT CAGGCCAGTG
    TCCTCCCCTG TGCTGTCTTC TTCAAACAAG AGCCCGTCCA GTGCTTGGAG CAGCAGCAGC
    TGGCATGGAA GGATCAAAGG AGGAATGAAG GGTTTTCAGA GCTTCATGGT TTCAGACAGT
    AACATGAGTT TTGTTGAATT TGTTGAGCTG TTCAAATCAT TCAGTGTGAG GAGCCGCAAG
    GACCTGAAGG ATCTCTTTGA CATCTACGCT GTGCCCTGCA ATCGGGCCGG CTCTGAGTCG
    GCCCCGCTCT ACACCAACCT AACAATAGAC GAAAACACCA GCGATCTTCA GCCTGACCTA
    GATTTATTGA CCAGAAACGT CTCGGATTTG GGGTTGTTCA TTAAGAGTAA ACAGCAGCTG
    TCAGACAACC AGAGGCAGAT TTCTGATGCC ATTGCTGCTG CAAGTATCGT GACGAATGGC
    ACTGGGATCG AGAGCACATC TTTGGGCATC TTTGGGGTTG GCATACTCCA GCTCAATGAC
    TTCCTAGTCA ACTGCCAGGG AGAACACTGC ACTTACGATG AAATCCTCAG CATCATCCAG
    AAGTTCGAGC CTAGCATTAG TATGTGTCAT CAGGGCCTAC TGTCCTTTGA AGGATTTGCA
    AGGTTTCTGA TGGATAAAGA TAATTTTGCC TCCAAAAATG ATGAGTCACA GGAGAACATT
    AAAGATTTGC AGCTGCCTCT CTCCTACTAT TACATTGAGT CTTCACACAA TACCTACCTC
    ACAGGCCACC AGCTCAAAGG AGAGTCATCG GTAGAACTCT ATAGCCAGGT CCTCCTGCAA
    GGCTGTAGAA GTGTAGAGTT GGACTGCTGG GATGGAGATG ACGGGATGCC CATCATCTAT
    CACGGACACA CACTAACAAC CAAGATCCCC TTCAAGGAGG TGGTTGAAGC CATCGACCGC
    AGTGCCTTCA TCAACTCTGA CCTGCCCATC ATCATATCGA TTGAGAACCA CTGCTCGTTG
    CCTCAGCAAC GAAAAATGGC AGAAATTTTC AAGACTGTGT TTGGAGAAAA ACTGGTGGCT
    AAATTCCTGT TTGAGAGCGA TTTCTCAGAT GATCCAATGC TTCCCTCACC TGACCAACTT
    AGGAGGAAAG TGCTTCTTAA AAATAAGAAG CTAAAAGCCC ACCAGACACC AGTGGATATC
    TTAAAGCAAA AGGCTCATCA GTTAGCATCC ATGCAAGCTC AGGCTTATAA TGGGGGAAAT
    GTCAACCCTC CACCTGCTAA TAACGAGGAA GAGGAAGATG AAGAGGACGA ATATGATTAT
    GACTATGAAT CCCTGTCTGA TGCAGATGTC CTTACTGCTT CTCCTGCTCC TAACAGTCAG
    GAAGACAACA TTCTAGAAGA CAGACCTGAA AATAAATCAT GTAATGACAA GCTTCAGTTT
    GAGTATAACG AAGAAATCCC CAAGAGGATA AAGAAAGCAG ATAATTCTGC TTTCAACAAA
    GGAAAGGTTT ATGACATGGA ACTGGGAGAA GAATTTTATC TTCCTCAGAA TAAAAAGGAA
    AGCAGACAGA TTGCACCAGA GCTTTCTGAC CTTGTCATCT ACTGCCAAGC GGTAAAGTTT
    CCGGGGCTGT CAACTCTAAA TGCATCTGGC TCTAACAGAG GAAAAGAGAG GAAAAGCAGG
    AAGTCCATTT TTGGCAACAA TCCGGGCAGA ATGAGCCCTG GGGAGACAGC ATCGTTTAAC
    AAAGCATCTG GAAAAAGTTC CTGTGAAGGA ATGCGACAGG CGTGGGAGGA ATCTTCTTCC
    CCCCTCAACC CAACCACATC CCTCAGCGCT ATCATTAGAA CTCCCAAATG TTACCATATC
    TCATCGCTGA ATGAAAATGC CGCCAAACGT CTGTGTCGCA GGTATTCTCA GAAACTGATC
    CAGCACACTG CCTGTCAGCT GCTGAGGACT TACCCGGCTG CCACACGCAT CGACTCCTCC
    AACCCCAATC CTCTCTTGTT CTGGCTCCAT GGGATACAGC TCGTGGCACT CAACTACCAG
    ACAGATGATC TCCCTTTACA TTTAAATGCT GCCATGTTTG AGGCCAACGG TGGCTGTGGT
    TATGTTTTGA AACCCCCCGT TCTGTGGGAC AAGAACTGTC CCATGTACCA GAAGTTTTCA
    CCCTTGGAAA GAGACCTGGA CAACATGGAG CCTGCCGTCT ATTCCTTAAC TATTGTCTCT
    GGTCAAAACG TGTGCCCAAG TAGCAGCACG GGAAGCCCAT GTATCGAAGT GGACGTGCTG
    GGCATGCCCC TGGACAGTTG CCACTTCCGC ACAAAACCCA TCCACCGGAA CACTTTGAAC
    CCCATGTGGA ATGAACAGTT TCTCTTCCGG GTTCACTTTG AAGATCTCAT ATTCCTGCGT
    TTTGCAGTTG TGGAAAACAA TAGCTCAGCC ATAACCGCTC AGAGAATCAT CCCCCTCAAG
    GCTTTAAAAC GAGGATATCG ACATCTTCAG CTGCGAAACC TCCACAATGA AGTCTTGGAA
    ATCTCTAGTT TATTCATAAA CAGCAGAAGG ATGGAAGAAA ATTCCTCCAG CAGTGCCACG
    CCAGCCTCTT TGATGTTTAA TACAGAAGAA AGAAAATGTT TGCAGACTCA CAGAGTCACA
    GTGCATGGGG TCCCAGGTCC AGAGCCCTTT ACTGTTTTCT CCATAAGTGG AGGCACGAAG
    GCAAAGCAGC TTCTCCAACA GATTCTAACT ACTGAACAAG ACACCAAACC TATTGTCACA
    GACTATTTTT TGATGGAAGA AAAATATTTT ATATCTAAAG AAAAGAATGA ATGCAGGAAA
    CAACCATTTC AGAGAGTCAT CGGTCCAGAA GAAGAGATCA TGCAGATTTT AAGCAGCTGG
    TTTCCAGAGG AAGGATATGT TGGCAGGATT GTCTTAAAAA CCCAGCAGGA AAACCTAGAA
    GAGAGAAGCA TTGTTCAAGA TGAGAAAGAG GTGATCTTGA GCTCGGAGGA GGAGAGCTTC
    TTTGTCCAAG TGCATGACGT TTCTCCAGAG CAACCTCGGA CGGTCATCAA AGCGCCCCGT
    GTCAGCACCG CTCAGGATGT CATTCAACAG ACCTTATGCA AAGCCAAATA TTCCTATAGC
    ATCCTGAGTA ACCCCAACCC GAGTGACTAT GTGCTTCTGG AAGAGGTGGT GAAAGATGCT
    ACCAAGAAGT CCTCCACCCC AAAGTCCTCC CAGCGGGTCC TTTTGGACCA AGAGTGTGTG
    TTTCAAGCCC AGAGCAAGTG GAAAGGTGCT GGAAAATTCA TCCTCAGGCT AAAGGAGCAG
    GTGCAGGCAT CCCGAGAAGA TAAAAGAAAA GGCATTTCTT TCGCAAGTGA ATTCAAGAAG
    TTCACCAAAT CAACTAAACA GCCCCGAGGA CTCACATCTT CTCAGGTCTT GGCCTCAGAA
    AGTGTCCAAA ACAAGGAGGA GAAGCCCACG GGCAGCTTGT CCTCCAGTGA CACCACAGAC
    TCCCGACAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27695
    Clone ID Related Accession (Same CDS sequence) XM_022411584.1 , XM_005637458.2 , XM_022411585.1
    Accession Version XM_005637458.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6972bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005637458.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGACTTCTG AAGGAATGGC AGCTTCTGTT CTCACACCTG TGACTCAAAG AAAAGTGACA 
    TTTGCTCCAT CGGCTGTAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAG TGTTCCAAAG
    GCACATAGTG TCAAGCAGAG TGAGCAGACT TCTACCATCC CGTGGATGAA CAAGCTTAAA
    GAAGAATCTT CTGGAAGCAA CTTGCCCAAA ATTCTCTCCA TAGCAAGGGA GAAAATAGCA
    AGTGATGAGA ACAGCAATGA AGAGTGCTGG ACGGAAAGCA CACCAGTTTC TGTGAAAAAC
    CTTAACATTA ACCATAACAA CATCCTGACA AACCGCCAGT GCGTCCTTCC TCAGAGCCAG
    TCTTATGACA CGTGCAATTC TGTCATGGAG GAAGACCCAT GTTTAGAAAC TGGAATCTCA
    TCCTCACTGG AAAGAAAGGT ATTCCCTGGA ATTCAACTGG AAGTCAACAG ACCTCCCATG
    GACTTTAGGC CTCCAGGCCT AATGGACTTT AGTACCTTAG GAAGTCAGTC AGCCATTGTA
    GATACTGGCC AGGCGCACCC TGACAGCAAC AAAGCAGCAT TTCAAATCTT TAATTATAAA
    GTTGACAGAA GGATGTCTGA CACTTTCTGT ACCCTGTCAG GTGACTTGAT ATTGGATGAC
    TGTGGAAACT GTGTGCCACT GTCATCTGGT TTTGGCGGGG AGCAAAAGAA AAATTACGTG
    GCATATACCT GTAAACTCAT GGAATTGGCT GAAAACTGTG ATAATGAGAA TGGACAGCTG
    CAGTGTGATG ATTATGATGC CTTGGATGAC AAATACTTGT GCTTTGAAGA TTCTTGCCAA
    AGGGACAGTG TGGTATGTTC AAGTGACAGC TTTCGCAGGG AGGACCTAAC TAACAGTCCA
    CCTGCCAAGA CCTTTCTGAG CCATTTTGAA GACTTCCCTG ATAATGGTGA AGATGTAGAA
    GATTTTTTAA AAAACAAAAA GGAGAGATCC ACTTTGTTAG TGAGGAGATT TTGTAAAAAT
    GACAGAGAAG TAAAGAAATC TGTGTATACT GGGACAAGGG CAATCATGAG AACTCTGCCT
    TCTGGTCACA TTGGGCTGGA AGCTTACAGT TACATCGATC AGAAGAGAAG TGGTCCCTTA
    TTACCCCGTG GGAGAGTTCT GGAACAGCTG CCAGTGGTGG CAATTAGGCA AGATGGGAGC
    CAGTGCCTGT CAGAAGCCCA GTGGTATCGG ATCTACAATG CAGTGAGAAG GGAAGAAGAA
    ATAGAAAATA CCATTGGATC TCTTCTCCAT TACTTTACCA AGCTCCCAGC CTCCAAGACA
    GCCCATGAAA GGATTAGTGT TGGTCCATGC CTAAAGCAGT GTGTCCGAGA CACCATATGT
    GAGTATCGTG CCACCCTCCA GAGGACGTCC ATATCTCAGT ACATCACGGG TTCGCTCCTA
    GAAGCAACCA CATCTTTAGG AGCGAGAAGT GGCCTACTCA GTACTTTTGG AGGATCCACT
    GGACGAATGA TGCTAAAAGA ACGCCAGCCA GGCACCTCCA TGGCCAATTC CAGTGCCCTC
    CCTTCAAGTT CCGCTGGAAT CAGCAAGGAG CTGATTGACC TGCAGCCTCT CATTCAGTTC
    CCAGAGGAAG TCGCCAGCAT CCTGATGGAG CAGGAACAGA ACATTTACCG AAGGGTCTTA
    CCAGTTGACT ATCTTTACTT CTTGACCCGG GACTTGGGCA CTCCAGAATG CCAGACACCC
    CTGCCCTGCC TCAAAGCATC CATCTCAGCT TCAATTCTTA GCTCCCAGAA CGGAGAGCAC
    AATGCCCTGG AAGATCTTGT GATGAGATTT AATGAGGTGA GCTCCTGGGT GACATGGCTG
    ATCCTCACGG CGGGCTCCAT GGAGGAGAAG CGAGAAGTCT TCTCCTATTT GGTGCATGTG
    GCCAAATGCT GCTGGAACAT GGGCAACTAC AATGCTGTCA TGGAGTTCTT GGCTGGTCTC
    AGGTCAAGAA AAGTTTTAAA AATGTGGCAG TTTATGGACC AATCTGACCT CGAGACCATG
    AGGAGCCTGA AGGACGCCAT GGCTCAGCAT GAATCTTCCT GCGAATATCG GAAGGTGATA
    ACGAGGGCTC TGCACATACC TGGCTGTAAA GTGGTTCCAT TCTGTGGAGT GTTTCTGAAG
    GAGCTCTGTG AAGTACTGGA CGGAGCCTCT GGCCTCATGA AGCTTTGCCC GAGGTACAAT
    TCCCAAGAAG AAACTCTAGA GTTTGTAGCA GATTACAGTG GACAAGATAA TTTCTTACAA
    CGAGTGGGGC ATAATGGCTT AAAGAATTCA GAGAAGGAGT CCACTGTCAA CAGCATCTTT
    CAAATCATTA GGAGCTGCAG CCGAAGTCTG GAGGCTGAGG AAGAGGACAG CCCCAGTGAA
    GGGAACAGCT CTAGGAAAAA TTCTTTAAGG GACAAAGCCC GATGGCAATT TATAATCGGA
    GATTTGTTGG ATTCCGAAAA TGACATCTTT GAGCAATCCA AAGAATGGGA CTCTCCCAGC
    TCAGAAGAGC CTCAGAAAGC CTTTGACCAT GGGACAGAGC TGATCCCTTG GTACGTGCTG
    TCCATCCGAG CAGATGTGCA CCAGTTCCTG CTACAGGGCG CCACCGTCCT CCGCTACGAC
    CAGGACACGC ACCTCTCTGC CCGCTGCTTC CTCCAGCTTC AGCCTGACAA TAGCACCTTG
    ACATGGATAA AGCCCACAGC TGCCTCCCCG GCCAGTGCTA GAGCAAAACT CGGTGTGCTG
    AGCAACACGT CTGAGCCTGG CAAGTTCCCG TCACCAGGCA GTGCCGGACT AAGCGGCCTG
    GCCGAGGGGG TCCTGGATCT GTTTTCAGCA AAGGCTGTGT ACATGGGACA CCCTAGCATT
    GATATCCACA CTGTGTGTGT CCAGAACAAA CTCGGTAGCA TGTTTCTCTC AGAGACTGGC
    GTGACCCTGC TCTACGGGCT CCAGACCACA GACAATAGAT TATTGCACTT TGTGGCACCC
    AAACACACTG CTAAGATGCT CTTCAGTGGA TTGCTAGAAC TCACGAGGGC CGTGAGGAAG
    ATGAGAAAGT TCCCTGACCA GAGGCAGCAG TGGCTGCGGA AACAGTATGT TAGCCTCTAT
    CAGGAGGACG GACGGTATGA GGGCCCAACT TTGGCTCATG CTGTGGAGTT GTTTGGTGGC
    CGGCGATGGA GTACACGCAA TCCCAGCCCT GGGACATCTG CAAAGAGTGC TGAGAAGCCC
    AGTGTGCAGA GAAACAACAC CTTGGGCATA AGCACCACCA AGAAAAAGAA GAAAATCCTT
    ATAAGGGGTG AGAGTGGAGA GGCAGCCGAC GATGAGATGG CCACCCGGAA GGCCAAAATG
    CACAGGGAGT GTCGCAGCCG GAGTGGTTCT GACCCACAAG ACATGAATGA GCAAGAAGAA
    TCTGAGGCAA ATGCCATCAT GAGCCCTCCT AACACTCTGC CTTCCAGAAG AGCCCACTCT
    TTGACTACAG CTGGGTCCCC TAATTTGTCT GCCGGGACAT CATCTCCCAT CAGGCCAGTG
    TCCTCCCCTG TGCTGTCTTC TTCAAACAAG AGCCCGTCCA GTGCTTGGAG CAGCAGCAGC
    TGGCATGGAA GGATCAAAGG AGGAATGAAG GGTTTTCAGA GCTTCATGGT TTCAGACAGT
    AACATGAGTT TTGTTGAATT TGTTGAGCTG TTCAAATCAT TCAGTGTGAG GAGCCGCAAG
    GACCTGAAGG ATCTCTTTGA CATCTACGCT GTGCCCTGCA ATCGGGCCGG CTCTGAGTCG
    GCCCCGCTCT ACACCAACCT AACAATAGAC GAAAACACCA GCGATCTTCA GCCTGACCTA
    GATTTATTGA CCAGAAACGT CTCGGATTTG GGGTTGTTCA TTAAGAGTAA ACAGCAGCTG
    TCAGACAACC AGAGGCAGAT TTCTGATGCC ATTGCTGCTG CAAGTATCGT GACGAATGGC
    ACTGGGATCG AGAGCACATC TTTGGGCATC TTTGGGGTTG GCATACTCCA GCTCAATGAC
    TTCCTAGTCA ACTGCCAGGG AGAACACTGC ACTTACGATG AAATCCTCAG CATCATCCAG
    AAGTTCGAGC CTAGCATTAG TATGTGTCAT CAGGGCCTAC TGTCCTTTGA AGGATTTGCA
    AGGTTTCTGA TGGATAAAGA TAATTTTGCC TCCAAAAATG ATGAGTCACA GGAGAACATT
    AAAGATTTGC AGCTGCCTCT CTCCTACTAT TACATTGAGT CTTCACACAA TACCTACCTC
    ACAGGCCACC AGCTCAAAGG AGAGTCATCG GTAGAACTCT ATAGCCAGGT CCTCCTGCAA
    GGCTGTAGAA GTGTAGAGTT GGACTGCTGG GATGGAGATG ACGGGATGCC CATCATCTAT
    CACGGACACA CACTAACAAC CAAGATCCCC TTCAAGGAGG TGGTTGAAGC CATCGACCGC
    AGTGCCTTCA TCAACTCTGA CCTGCCCATC ATCATATCGA TTGAGAACCA CTGCTCGTTG
    CCTCAGCAAC GAAAAATGGC AGAAATTTTC AAGACTGTGT TTGGAGAAAA ACTGGTGGCT
    AAATTCCTGT TTGAGAGCGA TTTCTCAGAT GATCCAATGC TTCCCTCACC TGACCAACTT
    AGGAGGAAAG TGCTTCTTAA AAATAAGAAG CTAAAAGCCC ACCAGACACC AGTGGATATC
    TTAAAGCAAA AGGCTCATCA GTTAGCATCC ATGCAAGCTC AGGCTTATAA TGGGGGAAAT
    GTCAACCCTC CACCTGCTAA TAACGAGGAA GAGGAAGATG AAGAGGACGA ATATGATTAT
    GACTATGAAT CCCTGTCTGA TGCAGATGTC CTTACTGCTT CTCCTGCTCC TAACAGTCAG
    GAAGACAACA TTCTAGAAGA CAGACCTGAA AATAAATCAT GTAATGACAA GCTTCAGTTT
    GAGTATAACG AAGAAATCCC CAAGAGGATA AAGAAAGCAG ATAATTCTGC TTTCAACAAA
    GGAAAGGTTT ATGACATGGA ACTGGGAGAA GAATTTTATC TTCCTCAGAA TAAAAAGGAA
    AGCAGACAGA TTGCACCAGA GCTTTCTGAC CTTGTCATCT ACTGCCAAGC GGTAAAGTTT
    CCGGGGCTGT CAACTCTAAA TGCATCTGGC TCTAACAGAG GAAAAGAGAG GAAAAGCAGG
    AAGTCCATTT TTGGCAACAA TCCGGGCAGA ATGAGCCCTG GGGAGACAGC ATCGTTTAAC
    AAAGCATCTG GAAAAAGTTC CTGTGAAGGA ATGCGACAGG CGTGGGAGGA ATCTTCTTCC
    CCCCTCAACC CAACCACATC CCTCAGCGCT ATCATTAGAA CTCCCAAATG TTACCATATC
    TCATCGCTGA ATGAAAATGC CGCCAAACGT CTGTGTCGCA GGTATTCTCA GAAACTGATC
    CAGCACACTG CCTGTCAGCT GCTGAGGACT TACCCGGCTG CCACACGCAT CGACTCCTCC
    AACCCCAATC CTCTCTTGTT CTGGCTCCAT GGGATACAGC TCGTGGCACT CAACTACCAG
    ACAGATGATC TCCCTTTACA TTTAAATGCT GCCATGTTTG AGGCCAACGG TGGCTGTGGT
    TATGTTTTGA AACCCCCCGT TCTGTGGGAC AAGAACTGTC CCATGTACCA GAAGTTTTCA
    CCCTTGGAAA GAGACCTGGA CAACATGGAG CCTGCCGTCT ATTCCTTAAC TATTGTCTCT
    GGTCAAAACG TGTGCCCAAG TAGCAGCACG GGAAGCCCAT GTATCGAAGT GGACGTGCTG
    GGCATGCCCC TGGACAGTTG CCACTTCCGC ACAAAACCCA TCCACCGGAA CACTTTGAAC
    CCCATGTGGA ATGAACAGTT TCTCTTCCGG GTTCACTTTG AAGATCTCAT ATTCCTGCGT
    TTTGCAGTTG TGGAAAACAA TAGCTCAGCC ATAACCGCTC AGAGAATCAT CCCCCTCAAG
    GCTTTAAAAC GAGGATATCG ACATCTTCAG CTGCGAAACC TCCACAATGA AGTCTTGGAA
    ATCTCTAGTT TATTCATAAA CAGCAGAAGG ATGGAAGAAA ATTCCTCCAG CAGTGCCACG
    CCAGCCTCTT TGATGTTTAA TACAGAAGAA AGAAAATGTT TGCAGACTCA CAGAGTCACA
    GTGCATGGGG TCCCAGGTCC AGAGCCCTTT ACTGTTTTCT CCATAAGTGG AGGCACGAAG
    GCAAAGCAGC TTCTCCAACA GATTCTAACT ACTGAACAAG ACACCAAACC TATTGTCACA
    GACTATTTTT TGATGGAAGA AAAATATTTT ATATCTAAAG AAAAGAATGA ATGCAGGAAA
    CAACCATTTC AGAGAGTCAT CGGTCCAGAA GAAGAGATCA TGCAGATTTT AAGCAGCTGG
    TTTCCAGAGG AAGGATATGT TGGCAGGATT GTCTTAAAAA CCCAGCAGGA AAACCTAGAA
    GAGAGAAGCA TTGTTCAAGA TGAGAAAGAG GTGATCTTGA GCTCGGAGGA GGAGAGCTTC
    TTTGTCCAAG TGCATGACGT TTCTCCAGAG CAACCTCGGA CGGTCATCAA AGCGCCCCGT
    GTCAGCACCG CTCAGGATGT CATTCAACAG ACCTTATGCA AAGCCAAATA TTCCTATAGC
    ATCCTGAGTA ACCCCAACCC GAGTGACTAT GTGCTTCTGG AAGAGGTGGT GAAAGATGCT
    ACCAAGAAGT CCTCCACCCC AAAGTCCTCC CAGCGGGTCC TTTTGGACCA AGAGTGTGTG
    TTTCAAGCCC AGAGCAAGTG GAAAGGTGCT GGAAAATTCA TCCTCAGGCT AAAGGAGCAG
    GTGCAGGCAT CCCGAGAAGA TAAAAGAAAA GGCATTTCTT TCGCAAGTGA ATTCAAGAAG
    TTCACCAAAT CAACTAAACA GCCCCGAGGA CTCACATCTT CTCAGGTCTT GGCCTCAGAA
    AGTGTCCAAA ACAAGGAGGA GAAGCCCACG GGCAGCTTGT CCTCCAGTGA CACCACAGAC
    TCCCGACAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005637515.1
    CDS556..7527
    Translation

    Target ORF information:

    RefSeq Version XM_005637458.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris phospholipase C, epsilon 1 (PLCE1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005637458.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGACTTCTG AAGGAATGGC AGCTTCTGTT CTCACACCTG TGACTCAAAG AAAAGTGACA 
    TTTGCTCCAT CGGCTGTAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAG TGTTCCAAAG
    GCACATAGTG TCAAGCAGAG TGAGCAGACT TCTACCATCC CGTGGATGAA CAAGCTTAAA
    GAAGAATCTT CTGGAAGCAA CTTGCCCAAA ATTCTCTCCA TAGCAAGGGA GAAAATAGCA
    AGTGATGAGA ACAGCAATGA AGAGTGCTGG ACGGAAAGCA CACCAGTTTC TGTGAAAAAC
    CTTAACATTA ACCATAACAA CATCCTGACA AACCGCCAGT GCGTCCTTCC TCAGAGCCAG
    TCTTATGACA CGTGCAATTC TGTCATGGAG GAAGACCCAT GTTTAGAAAC TGGAATCTCA
    TCCTCACTGG AAAGAAAGGT ATTCCCTGGA ATTCAACTGG AAGTCAACAG ACCTCCCATG
    GACTTTAGGC CTCCAGGCCT AATGGACTTT AGTACCTTAG GAAGTCAGTC AGCCATTGTA
    GATACTGGCC AGGCGCACCC TGACAGCAAC AAAGCAGCAT TTCAAATCTT TAATTATAAA
    GTTGACAGAA GGATGTCTGA CACTTTCTGT ACCCTGTCAG GTGACTTGAT ATTGGATGAC
    TGTGGAAACT GTGTGCCACT GTCATCTGGT TTTGGCGGGG AGCAAAAGAA AAATTACGTG
    GCATATACCT GTAAACTCAT GGAATTGGCT GAAAACTGTG ATAATGAGAA TGGACAGCTG
    CAGTGTGATG ATTATGATGC CTTGGATGAC AAATACTTGT GCTTTGAAGA TTCTTGCCAA
    AGGGACAGTG TGGTATGTTC AAGTGACAGC TTTCGCAGGG AGGACCTAAC TAACAGTCCA
    CCTGCCAAGA CCTTTCTGAG CCATTTTGAA GACTTCCCTG ATAATGGTGA AGATGTAGAA
    GATTTTTTAA AAAACAAAAA GGAGAGATCC ACTTTGTTAG TGAGGAGATT TTGTAAAAAT
    GACAGAGAAG TAAAGAAATC TGTGTATACT GGGACAAGGG CAATCATGAG AACTCTGCCT
    TCTGGTCACA TTGGGCTGGA AGCTTACAGT TACATCGATC AGAAGAGAAG TGGTCCCTTA
    TTACCCCGTG GGAGAGTTCT GGAACAGCTG CCAGTGGTGG CAATTAGGCA AGATGGGAGC
    CAGTGCCTGT CAGAAGCCCA GTGGTATCGG ATCTACAATG CAGTGAGAAG GGAAGAAGAA
    ATAGAAAATA CCATTGGATC TCTTCTCCAT TACTTTACCA AGCTCCCAGC CTCCAAGACA
    GCCCATGAAA GGATTAGTGT TGGTCCATGC CTAAAGCAGT GTGTCCGAGA CACCATATGT
    GAGTATCGTG CCACCCTCCA GAGGACGTCC ATATCTCAGT ACATCACGGG TTCGCTCCTA
    GAAGCAACCA CATCTTTAGG AGCGAGAAGT GGCCTACTCA GTACTTTTGG AGGATCCACT
    GGACGAATGA TGCTAAAAGA ACGCCAGCCA GGCACCTCCA TGGCCAATTC CAGTGCCCTC
    CCTTCAAGTT CCGCTGGAAT CAGCAAGGAG CTGATTGACC TGCAGCCTCT CATTCAGTTC
    CCAGAGGAAG TCGCCAGCAT CCTGATGGAG CAGGAACAGA ACATTTACCG AAGGGTCTTA
    CCAGTTGACT ATCTTTACTT CTTGACCCGG GACTTGGGCA CTCCAGAATG CCAGACACCC
    CTGCCCTGCC TCAAAGCATC CATCTCAGCT TCAATTCTTA GCTCCCAGAA CGGAGAGCAC
    AATGCCCTGG AAGATCTTGT GATGAGATTT AATGAGGTGA GCTCCTGGGT GACATGGCTG
    ATCCTCACGG CGGGCTCCAT GGAGGAGAAG CGAGAAGTCT TCTCCTATTT GGTGCATGTG
    GCCAAATGCT GCTGGAACAT GGGCAACTAC AATGCTGTCA TGGAGTTCTT GGCTGGTCTC
    AGGTCAAGAA AAGTTTTAAA AATGTGGCAG TTTATGGACC AATCTGACCT CGAGACCATG
    AGGAGCCTGA AGGACGCCAT GGCTCAGCAT GAATCTTCCT GCGAATATCG GAAGGTGATA
    ACGAGGGCTC TGCACATACC TGGCTGTAAA GTGGTTCCAT TCTGTGGAGT GTTTCTGAAG
    GAGCTCTGTG AAGTACTGGA CGGAGCCTCT GGCCTCATGA AGCTTTGCCC GAGGTACAAT
    TCCCAAGAAG AAACTCTAGA GTTTGTAGCA GATTACAGTG GACAAGATAA TTTCTTACAA
    CGAGTGGGGC ATAATGGCTT AAAGAATTCA GAGAAGGAGT CCACTGTCAA CAGCATCTTT
    CAAATCATTA GGAGCTGCAG CCGAAGTCTG GAGGCTGAGG AAGAGGACAG CCCCAGTGAA
    GGGAACAGCT CTAGGAAAAA TTCTTTAAGG GACAAAGCCC GATGGCAATT TATAATCGGA
    GATTTGTTGG ATTCCGAAAA TGACATCTTT GAGCAATCCA AAGAATGGGA CTCTCCCAGC
    TCAGAAGAGC CTCAGAAAGC CTTTGACCAT GGGACAGAGC TGATCCCTTG GTACGTGCTG
    TCCATCCGAG CAGATGTGCA CCAGTTCCTG CTACAGGGCG CCACCGTCCT CCGCTACGAC
    CAGGACACGC ACCTCTCTGC CCGCTGCTTC CTCCAGCTTC AGCCTGACAA TAGCACCTTG
    ACATGGATAA AGCCCACAGC TGCCTCCCCG GCCAGTGCTA GAGCAAAACT CGGTGTGCTG
    AGCAACACGT CTGAGCCTGG CAAGTTCCCG TCACCAGGCA GTGCCGGACT AAGCGGCCTG
    GCCGAGGGGG TCCTGGATCT GTTTTCAGCA AAGGCTGTGT ACATGGGACA CCCTAGCATT
    GATATCCACA CTGTGTGTGT CCAGAACAAA CTCGGTAGCA TGTTTCTCTC AGAGACTGGC
    GTGACCCTGC TCTACGGGCT CCAGACCACA GACAATAGAT TATTGCACTT TGTGGCACCC
    AAACACACTG CTAAGATGCT CTTCAGTGGA TTGCTAGAAC TCACGAGGGC CGTGAGGAAG
    ATGAGAAAGT TCCCTGACCA GAGGCAGCAG TGGCTGCGGA AACAGTATGT TAGCCTCTAT
    CAGGAGGACG GACGGTATGA GGGCCCAACT TTGGCTCATG CTGTGGAGTT GTTTGGTGGC
    CGGCGATGGA GTACACGCAA TCCCAGCCCT GGGACATCTG CAAAGAGTGC TGAGAAGCCC
    AGTGTGCAGA GAAACAACAC CTTGGGCATA AGCACCACCA AGAAAAAGAA GAAAATCCTT
    ATAAGGGGTG AGAGTGGAGA GGCAGCCGAC GATGAGATGG CCACCCGGAA GGCCAAAATG
    CACAGGGAGT GTCGCAGCCG GAGTGGTTCT GACCCACAAG ACATGAATGA GCAAGAAGAA
    TCTGAGGCAA ATGCCATCAT GAGCCCTCCT AACACTCTGC CTTCCAGAAG AGCCCACTCT
    TTGACTACAG CTGGGTCCCC TAATTTGTCT GCCGGGACAT CATCTCCCAT CAGGCCAGTG
    TCCTCCCCTG TGCTGTCTTC TTCAAACAAG AGCCCGTCCA GTGCTTGGAG CAGCAGCAGC
    TGGCATGGAA GGATCAAAGG AGGAATGAAG GGTTTTCAGA GCTTCATGGT TTCAGACAGT
    AACATGAGTT TTGTTGAATT TGTTGAGCTG TTCAAATCAT TCAGTGTGAG GAGCCGCAAG
    GACCTGAAGG ATCTCTTTGA CATCTACGCT GTGCCCTGCA ATCGGGCCGG CTCTGAGTCG
    GCCCCGCTCT ACACCAACCT AACAATAGAC GAAAACACCA GCGATCTTCA GCCTGACCTA
    GATTTATTGA CCAGAAACGT CTCGGATTTG GGGTTGTTCA TTAAGAGTAA ACAGCAGCTG
    TCAGACAACC AGAGGCAGAT TTCTGATGCC ATTGCTGCTG CAAGTATCGT GACGAATGGC
    ACTGGGATCG AGAGCACATC TTTGGGCATC TTTGGGGTTG GCATACTCCA GCTCAATGAC
    TTCCTAGTCA ACTGCCAGGG AGAACACTGC ACTTACGATG AAATCCTCAG CATCATCCAG
    AAGTTCGAGC CTAGCATTAG TATGTGTCAT CAGGGCCTAC TGTCCTTTGA AGGATTTGCA
    AGGTTTCTGA TGGATAAAGA TAATTTTGCC TCCAAAAATG ATGAGTCACA GGAGAACATT
    AAAGATTTGC AGCTGCCTCT CTCCTACTAT TACATTGAGT CTTCACACAA TACCTACCTC
    ACAGGCCACC AGCTCAAAGG AGAGTCATCG GTAGAACTCT ATAGCCAGGT CCTCCTGCAA
    GGCTGTAGAA GTGTAGAGTT GGACTGCTGG GATGGAGATG ACGGGATGCC CATCATCTAT
    CACGGACACA CACTAACAAC CAAGATCCCC TTCAAGGAGG TGGTTGAAGC CATCGACCGC
    AGTGCCTTCA TCAACTCTGA CCTGCCCATC ATCATATCGA TTGAGAACCA CTGCTCGTTG
    CCTCAGCAAC GAAAAATGGC AGAAATTTTC AAGACTGTGT TTGGAGAAAA ACTGGTGGCT
    AAATTCCTGT TTGAGAGCGA TTTCTCAGAT GATCCAATGC TTCCCTCACC TGACCAACTT
    AGGAGGAAAG TGCTTCTTAA AAATAAGAAG CTAAAAGCCC ACCAGACACC AGTGGATATC
    TTAAAGCAAA AGGCTCATCA GTTAGCATCC ATGCAAGCTC AGGCTTATAA TGGGGGAAAT
    GTCAACCCTC CACCTGCTAA TAACGAGGAA GAGGAAGATG AAGAGGACGA ATATGATTAT
    GACTATGAAT CCCTGTCTGA TGCAGATGTC CTTACTGCTT CTCCTGCTCC TAACAGTCAG
    GAAGACAACA TTCTAGAAGA CAGACCTGAA AATAAATCAT GTAATGACAA GCTTCAGTTT
    GAGTATAACG AAGAAATCCC CAAGAGGATA AAGAAAGCAG ATAATTCTGC TTTCAACAAA
    GGAAAGGTTT ATGACATGGA ACTGGGAGAA GAATTTTATC TTCCTCAGAA TAAAAAGGAA
    AGCAGACAGA TTGCACCAGA GCTTTCTGAC CTTGTCATCT ACTGCCAAGC GGTAAAGTTT
    CCGGGGCTGT CAACTCTAAA TGCATCTGGC TCTAACAGAG GAAAAGAGAG GAAAAGCAGG
    AAGTCCATTT TTGGCAACAA TCCGGGCAGA ATGAGCCCTG GGGAGACAGC ATCGTTTAAC
    AAAGCATCTG GAAAAAGTTC CTGTGAAGGA ATGCGACAGG CGTGGGAGGA ATCTTCTTCC
    CCCCTCAACC CAACCACATC CCTCAGCGCT ATCATTAGAA CTCCCAAATG TTACCATATC
    TCATCGCTGA ATGAAAATGC CGCCAAACGT CTGTGTCGCA GGTATTCTCA GAAACTGATC
    CAGCACACTG CCTGTCAGCT GCTGAGGACT TACCCGGCTG CCACACGCAT CGACTCCTCC
    AACCCCAATC CTCTCTTGTT CTGGCTCCAT GGGATACAGC TCGTGGCACT CAACTACCAG
    ACAGATGATC TCCCTTTACA TTTAAATGCT GCCATGTTTG AGGCCAACGG TGGCTGTGGT
    TATGTTTTGA AACCCCCCGT TCTGTGGGAC AAGAACTGTC CCATGTACCA GAAGTTTTCA
    CCCTTGGAAA GAGACCTGGA CAACATGGAG CCTGCCGTCT ATTCCTTAAC TATTGTCTCT
    GGTCAAAACG TGTGCCCAAG TAGCAGCACG GGAAGCCCAT GTATCGAAGT GGACGTGCTG
    GGCATGCCCC TGGACAGTTG CCACTTCCGC ACAAAACCCA TCCACCGGAA CACTTTGAAC
    CCCATGTGGA ATGAACAGTT TCTCTTCCGG GTTCACTTTG AAGATCTCAT ATTCCTGCGT
    TTTGCAGTTG TGGAAAACAA TAGCTCAGCC ATAACCGCTC AGAGAATCAT CCCCCTCAAG
    GCTTTAAAAC GAGGATATCG ACATCTTCAG CTGCGAAACC TCCACAATGA AGTCTTGGAA
    ATCTCTAGTT TATTCATAAA CAGCAGAAGG ATGGAAGAAA ATTCCTCCAG CAGTGCCACG
    CCAGCCTCTT TGATGTTTAA TACAGAAGAA AGAAAATGTT TGCAGACTCA CAGAGTCACA
    GTGCATGGGG TCCCAGGTCC AGAGCCCTTT ACTGTTTTCT CCATAAGTGG AGGCACGAAG
    GCAAAGCAGC TTCTCCAACA GATTCTAACT ACTGAACAAG ACACCAAACC TATTGTCACA
    GACTATTTTT TGATGGAAGA AAAATATTTT ATATCTAAAG AAAAGAATGA ATGCAGGAAA
    CAACCATTTC AGAGAGTCAT CGGTCCAGAA GAAGAGATCA TGCAGATTTT AAGCAGCTGG
    TTTCCAGAGG AAGGATATGT TGGCAGGATT GTCTTAAAAA CCCAGCAGGA AAACCTAGAA
    GAGAGAAGCA TTGTTCAAGA TGAGAAAGAG GTGATCTTGA GCTCGGAGGA GGAGAGCTTC
    TTTGTCCAAG TGCATGACGT TTCTCCAGAG CAACCTCGGA CGGTCATCAA AGCGCCCCGT
    GTCAGCACCG CTCAGGATGT CATTCAACAG ACCTTATGCA AAGCCAAATA TTCCTATAGC
    ATCCTGAGTA ACCCCAACCC GAGTGACTAT GTGCTTCTGG AAGAGGTGGT GAAAGATGCT
    ACCAAGAAGT CCTCCACCCC AAAGTCCTCC CAGCGGGTCC TTTTGGACCA AGAGTGTGTG
    TTTCAAGCCC AGAGCAAGTG GAAAGGTGCT GGAAAATTCA TCCTCAGGCT AAAGGAGCAG
    GTGCAGGCAT CCCGAGAAGA TAAAAGAAAA GGCATTTCTT TCGCAAGTGA ATTCAAGAAG
    TTCACCAAAT CAACTAAACA GCCCCGAGGA CTCACATCTT CTCAGGTCTT GGCCTCAGAA
    AGTGTCCAAA ACAAGGAGGA GAAGCCCACG GGCAGCTTGT CCTCCAGTGA CACCACAGAC
    TCCCGACAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27695
    Clone ID Related Accession (Same CDS sequence) XM_022411584.1 , XM_005637458.2 , XM_022411585.1
    Accession Version XM_022411585.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6972bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006610.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGACTTCTG AAGGAATGGC AGCTTCTGTT CTCACACCTG TGACTCAAAG AAAAGTGACA 
    TTTGCTCCAT CGGCTGTAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAG TGTTCCAAAG
    GCACATAGTG TCAAGCAGAG TGAGCAGACT TCTACCATCC CGTGGATGAA CAAGCTTAAA
    GAAGAATCTT CTGGAAGCAA CTTGCCCAAA ATTCTCTCCA TAGCAAGGGA GAAAATAGCA
    AGTGATGAGA ACAGCAATGA AGAGTGCTGG ACGGAAAGCA CACCAGTTTC TGTGAAAAAC
    CTTAACATTA ACCATAACAA CATCCTGACA AACCGCCAGT GCGTCCTTCC TCAGAGCCAG
    TCTTATGACA CGTGCAATTC TGTCATGGAG GAAGACCCAT GTTTAGAAAC TGGAATCTCA
    TCCTCACTGG AAAGAAAGGT ATTCCCTGGA ATTCAACTGG AAGTCAACAG ACCTCCCATG
    GACTTTAGGC CTCCAGGCCT AATGGACTTT AGTACCTTAG GAAGTCAGTC AGCCATTGTA
    GATACTGGCC AGGCGCACCC TGACAGCAAC AAAGCAGCAT TTCAAATCTT TAATTATAAA
    GTTGACAGAA GGATGTCTGA CACTTTCTGT ACCCTGTCAG GTGACTTGAT ATTGGATGAC
    TGTGGAAACT GTGTGCCACT GTCATCTGGT TTTGGCGGGG AGCAAAAGAA AAATTACGTG
    GCATATACCT GTAAACTCAT GGAATTGGCT GAAAACTGTG ATAATGAGAA TGGACAGCTG
    CAGTGTGATG ATTATGATGC CTTGGATGAC AAATACTTGT GCTTTGAAGA TTCTTGCCAA
    AGGGACAGTG TGGTATGTTC AAGTGACAGC TTTCGCAGGG AGGACCTAAC TAACAGTCCA
    CCTGCCAAGA CCTTTCTGAG CCATTTTGAA GACTTCCCTG ATAATGGTGA AGATGTAGAA
    GATTTTTTAA AAAACAAAAA GGAGAGATCC ACTTTGTTAG TGAGGAGATT TTGTAAAAAT
    GACAGAGAAG TAAAGAAATC TGTGTATACT GGGACAAGGG CAATCATGAG AACTCTGCCT
    TCTGGTCACA TTGGGCTGGA AGCTTACAGT TACATCGATC AGAAGAGAAG TGGTCCCTTA
    TTACCCCGTG GGAGAGTTCT GGAACAGCTG CCAGTGGTGG CAATTAGGCA AGATGGGAGC
    CAGTGCCTGT CAGAAGCCCA GTGGTATCGG ATCTACAATG CAGTGAGAAG GGAAGAAGAA
    ATAGAAAATA CCATTGGATC TCTTCTCCAT TACTTTACCA AGCTCCCAGC CTCCAAGACA
    GCCCATGAAA GGATTAGTGT TGGTCCATGC CTAAAGCAGT GTGTCCGAGA CACCATATGT
    GAGTATCGTG CCACCCTCCA GAGGACGTCC ATATCTCAGT ACATCACGGG TTCGCTCCTA
    GAAGCAACCA CATCTTTAGG AGCGAGAAGT GGCCTACTCA GTACTTTTGG AGGATCCACT
    GGACGAATGA TGCTAAAAGA ACGCCAGCCA GGCACCTCCA TGGCCAATTC CAGTGCCCTC
    CCTTCAAGTT CCGCTGGAAT CAGCAAGGAG CTGATTGACC TGCAGCCTCT CATTCAGTTC
    CCAGAGGAAG TCGCCAGCAT CCTGATGGAG CAGGAACAGA ACATTTACCG AAGGGTCTTA
    CCAGTTGACT ATCTTTACTT CTTGACCCGG GACTTGGGCA CTCCAGAATG CCAGACACCC
    CTGCCCTGCC TCAAAGCATC CATCTCAGCT TCAATTCTTA GCTCCCAGAA CGGAGAGCAC
    AATGCCCTGG AAGATCTTGT GATGAGATTT AATGAGGTGA GCTCCTGGGT GACATGGCTG
    ATCCTCACGG CGGGCTCCAT GGAGGAGAAG CGAGAAGTCT TCTCCTATTT GGTGCATGTG
    GCCAAATGCT GCTGGAACAT GGGCAACTAC AATGCTGTCA TGGAGTTCTT GGCTGGTCTC
    AGGTCAAGAA AAGTTTTAAA AATGTGGCAG TTTATGGACC AATCTGACCT CGAGACCATG
    AGGAGCCTGA AGGACGCCAT GGCTCAGCAT GAATCTTCCT GCGAATATCG GAAGGTGATA
    ACGAGGGCTC TGCACATACC TGGCTGTAAA GTGGTTCCAT TCTGTGGAGT GTTTCTGAAG
    GAGCTCTGTG AAGTACTGGA CGGAGCCTCT GGCCTCATGA AGCTTTGCCC GAGGTACAAT
    TCCCAAGAAG AAACTCTAGA GTTTGTAGCA GATTACAGTG GACAAGATAA TTTCTTACAA
    CGAGTGGGGC ATAATGGCTT AAAGAATTCA GAGAAGGAGT CCACTGTCAA CAGCATCTTT
    CAAATCATTA GGAGCTGCAG CCGAAGTCTG GAGGCTGAGG AAGAGGACAG CCCCAGTGAA
    GGGAACAGCT CTAGGAAAAA TTCTTTAAGG GACAAAGCCC GATGGCAATT TATAATCGGA
    GATTTGTTGG ATTCCGAAAA TGACATCTTT GAGCAATCCA AAGAATGGGA CTCTCCCAGC
    TCAGAAGAGC CTCAGAAAGC CTTTGACCAT GGGACAGAGC TGATCCCTTG GTACGTGCTG
    TCCATCCGAG CAGATGTGCA CCAGTTCCTG CTACAGGGCG CCACCGTCCT CCGCTACGAC
    CAGGACACGC ACCTCTCTGC CCGCTGCTTC CTCCAGCTTC AGCCTGACAA TAGCACCTTG
    ACATGGATAA AGCCCACAGC TGCCTCCCCG GCCAGTGCTA GAGCAAAACT CGGTGTGCTG
    AGCAACACGT CTGAGCCTGG CAAGTTCCCG TCACCAGGCA GTGCCGGACT AAGCGGCCTG
    GCCGAGGGGG TCCTGGATCT GTTTTCAGCA AAGGCTGTGT ACATGGGACA CCCTAGCATT
    GATATCCACA CTGTGTGTGT CCAGAACAAA CTCGGTAGCA TGTTTCTCTC AGAGACTGGC
    GTGACCCTGC TCTACGGGCT CCAGACCACA GACAATAGAT TATTGCACTT TGTGGCACCC
    AAACACACTG CTAAGATGCT CTTCAGTGGA TTGCTAGAAC TCACGAGGGC CGTGAGGAAG
    ATGAGAAAGT TCCCTGACCA GAGGCAGCAG TGGCTGCGGA AACAGTATGT TAGCCTCTAT
    CAGGAGGACG GACGGTATGA GGGCCCAACT TTGGCTCATG CTGTGGAGTT GTTTGGTGGC
    CGGCGATGGA GTACACGCAA TCCCAGCCCT GGGACATCTG CAAAGAGTGC TGAGAAGCCC
    AGTGTGCAGA GAAACAACAC CTTGGGCATA AGCACCACCA AGAAAAAGAA GAAAATCCTT
    ATAAGGGGTG AGAGTGGAGA GGCAGCCGAC GATGAGATGG CCACCCGGAA GGCCAAAATG
    CACAGGGAGT GTCGCAGCCG GAGTGGTTCT GACCCACAAG ACATGAATGA GCAAGAAGAA
    TCTGAGGCAA ATGCCATCAT GAGCCCTCCT AACACTCTGC CTTCCAGAAG AGCCCACTCT
    TTGACTACAG CTGGGTCCCC TAATTTGTCT GCCGGGACAT CATCTCCCAT CAGGCCAGTG
    TCCTCCCCTG TGCTGTCTTC TTCAAACAAG AGCCCGTCCA GTGCTTGGAG CAGCAGCAGC
    TGGCATGGAA GGATCAAAGG AGGAATGAAG GGTTTTCAGA GCTTCATGGT TTCAGACAGT
    AACATGAGTT TTGTTGAATT TGTTGAGCTG TTCAAATCAT TCAGTGTGAG GAGCCGCAAG
    GACCTGAAGG ATCTCTTTGA CATCTACGCT GTGCCCTGCA ATCGGGCCGG CTCTGAGTCG
    GCCCCGCTCT ACACCAACCT AACAATAGAC GAAAACACCA GCGATCTTCA GCCTGACCTA
    GATTTATTGA CCAGAAACGT CTCGGATTTG GGGTTGTTCA TTAAGAGTAA ACAGCAGCTG
    TCAGACAACC AGAGGCAGAT TTCTGATGCC ATTGCTGCTG CAAGTATCGT GACGAATGGC
    ACTGGGATCG AGAGCACATC TTTGGGCATC TTTGGGGTTG GCATACTCCA GCTCAATGAC
    TTCCTAGTCA ACTGCCAGGG AGAACACTGC ACTTACGATG AAATCCTCAG CATCATCCAG
    AAGTTCGAGC CTAGCATTAG TATGTGTCAT CAGGGCCTAC TGTCCTTTGA AGGATTTGCA
    AGGTTTCTGA TGGATAAAGA TAATTTTGCC TCCAAAAATG ATGAGTCACA GGAGAACATT
    AAAGATTTGC AGCTGCCTCT CTCCTACTAT TACATTGAGT CTTCACACAA TACCTACCTC
    ACAGGCCACC AGCTCAAAGG AGAGTCATCG GTAGAACTCT ATAGCCAGGT CCTCCTGCAA
    GGCTGTAGAA GTGTAGAGTT GGACTGCTGG GATGGAGATG ACGGGATGCC CATCATCTAT
    CACGGACACA CACTAACAAC CAAGATCCCC TTCAAGGAGG TGGTTGAAGC CATCGACCGC
    AGTGCCTTCA TCAACTCTGA CCTGCCCATC ATCATATCGA TTGAGAACCA CTGCTCGTTG
    CCTCAGCAAC GAAAAATGGC AGAAATTTTC AAGACTGTGT TTGGAGAAAA ACTGGTGGCT
    AAATTCCTGT TTGAGAGCGA TTTCTCAGAT GATCCAATGC TTCCCTCACC TGACCAACTT
    AGGAGGAAAG TGCTTCTTAA AAATAAGAAG CTAAAAGCCC ACCAGACACC AGTGGATATC
    TTAAAGCAAA AGGCTCATCA GTTAGCATCC ATGCAAGCTC AGGCTTATAA TGGGGGAAAT
    GTCAACCCTC CACCTGCTAA TAACGAGGAA GAGGAAGATG AAGAGGACGA ATATGATTAT
    GACTATGAAT CCCTGTCTGA TGCAGATGTC CTTACTGCTT CTCCTGCTCC TAACAGTCAG
    GAAGACAACA TTCTAGAAGA CAGACCTGAA AATAAATCAT GTAATGACAA GCTTCAGTTT
    GAGTATAACG AAGAAATCCC CAAGAGGATA AAGAAAGCAG ATAATTCTGC TTTCAACAAA
    GGAAAGGTTT ATGACATGGA ACTGGGAGAA GAATTTTATC TTCCTCAGAA TAAAAAGGAA
    AGCAGACAGA TTGCACCAGA GCTTTCTGAC CTTGTCATCT ACTGCCAAGC GGTAAAGTTT
    CCGGGGCTGT CAACTCTAAA TGCATCTGGC TCTAACAGAG GAAAAGAGAG GAAAAGCAGG
    AAGTCCATTT TTGGCAACAA TCCGGGCAGA ATGAGCCCTG GGGAGACAGC ATCGTTTAAC
    AAAGCATCTG GAAAAAGTTC CTGTGAAGGA ATGCGACAGG CGTGGGAGGA ATCTTCTTCC
    CCCCTCAACC CAACCACATC CCTCAGCGCT ATCATTAGAA CTCCCAAATG TTACCATATC
    TCATCGCTGA ATGAAAATGC CGCCAAACGT CTGTGTCGCA GGTATTCTCA GAAACTGATC
    CAGCACACTG CCTGTCAGCT GCTGAGGACT TACCCGGCTG CCACACGCAT CGACTCCTCC
    AACCCCAATC CTCTCTTGTT CTGGCTCCAT GGGATACAGC TCGTGGCACT CAACTACCAG
    ACAGATGATC TCCCTTTACA TTTAAATGCT GCCATGTTTG AGGCCAACGG TGGCTGTGGT
    TATGTTTTGA AACCCCCCGT TCTGTGGGAC AAGAACTGTC CCATGTACCA GAAGTTTTCA
    CCCTTGGAAA GAGACCTGGA CAACATGGAG CCTGCCGTCT ATTCCTTAAC TATTGTCTCT
    GGTCAAAACG TGTGCCCAAG TAGCAGCACG GGAAGCCCAT GTATCGAAGT GGACGTGCTG
    GGCATGCCCC TGGACAGTTG CCACTTCCGC ACAAAACCCA TCCACCGGAA CACTTTGAAC
    CCCATGTGGA ATGAACAGTT TCTCTTCCGG GTTCACTTTG AAGATCTCAT ATTCCTGCGT
    TTTGCAGTTG TGGAAAACAA TAGCTCAGCC ATAACCGCTC AGAGAATCAT CCCCCTCAAG
    GCTTTAAAAC GAGGATATCG ACATCTTCAG CTGCGAAACC TCCACAATGA AGTCTTGGAA
    ATCTCTAGTT TATTCATAAA CAGCAGAAGG ATGGAAGAAA ATTCCTCCAG CAGTGCCACG
    CCAGCCTCTT TGATGTTTAA TACAGAAGAA AGAAAATGTT TGCAGACTCA CAGAGTCACA
    GTGCATGGGG TCCCAGGTCC AGAGCCCTTT ACTGTTTTCT CCATAAGTGG AGGCACGAAG
    GCAAAGCAGC TTCTCCAACA GATTCTAACT ACTGAACAAG ACACCAAACC TATTGTCACA
    GACTATTTTT TGATGGAAGA AAAATATTTT ATATCTAAAG AAAAGAATGA ATGCAGGAAA
    CAACCATTTC AGAGAGTCAT CGGTCCAGAA GAAGAGATCA TGCAGATTTT AAGCAGCTGG
    TTTCCAGAGG AAGGATATGT TGGCAGGATT GTCTTAAAAA CCCAGCAGGA AAACCTAGAA
    GAGAGAAGCA TTGTTCAAGA TGAGAAAGAG GTGATCTTGA GCTCGGAGGA GGAGAGCTTC
    TTTGTCCAAG TGCATGACGT TTCTCCAGAG CAACCTCGGA CGGTCATCAA AGCGCCCCGT
    GTCAGCACCG CTCAGGATGT CATTCAACAG ACCTTATGCA AAGCCAAATA TTCCTATAGC
    ATCCTGAGTA ACCCCAACCC GAGTGACTAT GTGCTTCTGG AAGAGGTGGT GAAAGATGCT
    ACCAAGAAGT CCTCCACCCC AAAGTCCTCC CAGCGGGTCC TTTTGGACCA AGAGTGTGTG
    TTTCAAGCCC AGAGCAAGTG GAAAGGTGCT GGAAAATTCA TCCTCAGGCT AAAGGAGCAG
    GTGCAGGCAT CCCGAGAAGA TAAAAGAAAA GGCATTTCTT TCGCAAGTGA ATTCAAGAAG
    TTCACCAAAT CAACTAAACA GCCCCGAGGA CTCACATCTT CTCAGGTCTT GGCCTCAGAA
    AGTGTCCAAA ACAAGGAGGA GAAGCCCACG GGCAGCTTGT CCTCCAGTGA CACCACAGAC
    TCCCGACAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022267293.1
    CDS352..7323
    Translation

    Target ORF information:

    RefSeq Version XM_022411585.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris phospholipase C epsilon 1 (PLCE1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022411585.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGACTTCTG AAGGAATGGC AGCTTCTGTT CTCACACCTG TGACTCAAAG AAAAGTGACA 
    TTTGCTCCAT CGGCTGTAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAG TGTTCCAAAG
    GCACATAGTG TCAAGCAGAG TGAGCAGACT TCTACCATCC CGTGGATGAA CAAGCTTAAA
    GAAGAATCTT CTGGAAGCAA CTTGCCCAAA ATTCTCTCCA TAGCAAGGGA GAAAATAGCA
    AGTGATGAGA ACAGCAATGA AGAGTGCTGG ACGGAAAGCA CACCAGTTTC TGTGAAAAAC
    CTTAACATTA ACCATAACAA CATCCTGACA AACCGCCAGT GCGTCCTTCC TCAGAGCCAG
    TCTTATGACA CGTGCAATTC TGTCATGGAG GAAGACCCAT GTTTAGAAAC TGGAATCTCA
    TCCTCACTGG AAAGAAAGGT ATTCCCTGGA ATTCAACTGG AAGTCAACAG ACCTCCCATG
    GACTTTAGGC CTCCAGGCCT AATGGACTTT AGTACCTTAG GAAGTCAGTC AGCCATTGTA
    GATACTGGCC AGGCGCACCC TGACAGCAAC AAAGCAGCAT TTCAAATCTT TAATTATAAA
    GTTGACAGAA GGATGTCTGA CACTTTCTGT ACCCTGTCAG GTGACTTGAT ATTGGATGAC
    TGTGGAAACT GTGTGCCACT GTCATCTGGT TTTGGCGGGG AGCAAAAGAA AAATTACGTG
    GCATATACCT GTAAACTCAT GGAATTGGCT GAAAACTGTG ATAATGAGAA TGGACAGCTG
    CAGTGTGATG ATTATGATGC CTTGGATGAC AAATACTTGT GCTTTGAAGA TTCTTGCCAA
    AGGGACAGTG TGGTATGTTC AAGTGACAGC TTTCGCAGGG AGGACCTAAC TAACAGTCCA
    CCTGCCAAGA CCTTTCTGAG CCATTTTGAA GACTTCCCTG ATAATGGTGA AGATGTAGAA
    GATTTTTTAA AAAACAAAAA GGAGAGATCC ACTTTGTTAG TGAGGAGATT TTGTAAAAAT
    GACAGAGAAG TAAAGAAATC TGTGTATACT GGGACAAGGG CAATCATGAG AACTCTGCCT
    TCTGGTCACA TTGGGCTGGA AGCTTACAGT TACATCGATC AGAAGAGAAG TGGTCCCTTA
    TTACCCCGTG GGAGAGTTCT GGAACAGCTG CCAGTGGTGG CAATTAGGCA AGATGGGAGC
    CAGTGCCTGT CAGAAGCCCA GTGGTATCGG ATCTACAATG CAGTGAGAAG GGAAGAAGAA
    ATAGAAAATA CCATTGGATC TCTTCTCCAT TACTTTACCA AGCTCCCAGC CTCCAAGACA
    GCCCATGAAA GGATTAGTGT TGGTCCATGC CTAAAGCAGT GTGTCCGAGA CACCATATGT
    GAGTATCGTG CCACCCTCCA GAGGACGTCC ATATCTCAGT ACATCACGGG TTCGCTCCTA
    GAAGCAACCA CATCTTTAGG AGCGAGAAGT GGCCTACTCA GTACTTTTGG AGGATCCACT
    GGACGAATGA TGCTAAAAGA ACGCCAGCCA GGCACCTCCA TGGCCAATTC CAGTGCCCTC
    CCTTCAAGTT CCGCTGGAAT CAGCAAGGAG CTGATTGACC TGCAGCCTCT CATTCAGTTC
    CCAGAGGAAG TCGCCAGCAT CCTGATGGAG CAGGAACAGA ACATTTACCG AAGGGTCTTA
    CCAGTTGACT ATCTTTACTT CTTGACCCGG GACTTGGGCA CTCCAGAATG CCAGACACCC
    CTGCCCTGCC TCAAAGCATC CATCTCAGCT TCAATTCTTA GCTCCCAGAA CGGAGAGCAC
    AATGCCCTGG AAGATCTTGT GATGAGATTT AATGAGGTGA GCTCCTGGGT GACATGGCTG
    ATCCTCACGG CGGGCTCCAT GGAGGAGAAG CGAGAAGTCT TCTCCTATTT GGTGCATGTG
    GCCAAATGCT GCTGGAACAT GGGCAACTAC AATGCTGTCA TGGAGTTCTT GGCTGGTCTC
    AGGTCAAGAA AAGTTTTAAA AATGTGGCAG TTTATGGACC AATCTGACCT CGAGACCATG
    AGGAGCCTGA AGGACGCCAT GGCTCAGCAT GAATCTTCCT GCGAATATCG GAAGGTGATA
    ACGAGGGCTC TGCACATACC TGGCTGTAAA GTGGTTCCAT TCTGTGGAGT GTTTCTGAAG
    GAGCTCTGTG AAGTACTGGA CGGAGCCTCT GGCCTCATGA AGCTTTGCCC GAGGTACAAT
    TCCCAAGAAG AAACTCTAGA GTTTGTAGCA GATTACAGTG GACAAGATAA TTTCTTACAA
    CGAGTGGGGC ATAATGGCTT AAAGAATTCA GAGAAGGAGT CCACTGTCAA CAGCATCTTT
    CAAATCATTA GGAGCTGCAG CCGAAGTCTG GAGGCTGAGG AAGAGGACAG CCCCAGTGAA
    GGGAACAGCT CTAGGAAAAA TTCTTTAAGG GACAAAGCCC GATGGCAATT TATAATCGGA
    GATTTGTTGG ATTCCGAAAA TGACATCTTT GAGCAATCCA AAGAATGGGA CTCTCCCAGC
    TCAGAAGAGC CTCAGAAAGC CTTTGACCAT GGGACAGAGC TGATCCCTTG GTACGTGCTG
    TCCATCCGAG CAGATGTGCA CCAGTTCCTG CTACAGGGCG CCACCGTCCT CCGCTACGAC
    CAGGACACGC ACCTCTCTGC CCGCTGCTTC CTCCAGCTTC AGCCTGACAA TAGCACCTTG
    ACATGGATAA AGCCCACAGC TGCCTCCCCG GCCAGTGCTA GAGCAAAACT CGGTGTGCTG
    AGCAACACGT CTGAGCCTGG CAAGTTCCCG TCACCAGGCA GTGCCGGACT AAGCGGCCTG
    GCCGAGGGGG TCCTGGATCT GTTTTCAGCA AAGGCTGTGT ACATGGGACA CCCTAGCATT
    GATATCCACA CTGTGTGTGT CCAGAACAAA CTCGGTAGCA TGTTTCTCTC AGAGACTGGC
    GTGACCCTGC TCTACGGGCT CCAGACCACA GACAATAGAT TATTGCACTT TGTGGCACCC
    AAACACACTG CTAAGATGCT CTTCAGTGGA TTGCTAGAAC TCACGAGGGC CGTGAGGAAG
    ATGAGAAAGT TCCCTGACCA GAGGCAGCAG TGGCTGCGGA AACAGTATGT TAGCCTCTAT
    CAGGAGGACG GACGGTATGA GGGCCCAACT TTGGCTCATG CTGTGGAGTT GTTTGGTGGC
    CGGCGATGGA GTACACGCAA TCCCAGCCCT GGGACATCTG CAAAGAGTGC TGAGAAGCCC
    AGTGTGCAGA GAAACAACAC CTTGGGCATA AGCACCACCA AGAAAAAGAA GAAAATCCTT
    ATAAGGGGTG AGAGTGGAGA GGCAGCCGAC GATGAGATGG CCACCCGGAA GGCCAAAATG
    CACAGGGAGT GTCGCAGCCG GAGTGGTTCT GACCCACAAG ACATGAATGA GCAAGAAGAA
    TCTGAGGCAA ATGCCATCAT GAGCCCTCCT AACACTCTGC CTTCCAGAAG AGCCCACTCT
    TTGACTACAG CTGGGTCCCC TAATTTGTCT GCCGGGACAT CATCTCCCAT CAGGCCAGTG
    TCCTCCCCTG TGCTGTCTTC TTCAAACAAG AGCCCGTCCA GTGCTTGGAG CAGCAGCAGC
    TGGCATGGAA GGATCAAAGG AGGAATGAAG GGTTTTCAGA GCTTCATGGT TTCAGACAGT
    AACATGAGTT TTGTTGAATT TGTTGAGCTG TTCAAATCAT TCAGTGTGAG GAGCCGCAAG
    GACCTGAAGG ATCTCTTTGA CATCTACGCT GTGCCCTGCA ATCGGGCCGG CTCTGAGTCG
    GCCCCGCTCT ACACCAACCT AACAATAGAC GAAAACACCA GCGATCTTCA GCCTGACCTA
    GATTTATTGA CCAGAAACGT CTCGGATTTG GGGTTGTTCA TTAAGAGTAA ACAGCAGCTG
    TCAGACAACC AGAGGCAGAT TTCTGATGCC ATTGCTGCTG CAAGTATCGT GACGAATGGC
    ACTGGGATCG AGAGCACATC TTTGGGCATC TTTGGGGTTG GCATACTCCA GCTCAATGAC
    TTCCTAGTCA ACTGCCAGGG AGAACACTGC ACTTACGATG AAATCCTCAG CATCATCCAG
    AAGTTCGAGC CTAGCATTAG TATGTGTCAT CAGGGCCTAC TGTCCTTTGA AGGATTTGCA
    AGGTTTCTGA TGGATAAAGA TAATTTTGCC TCCAAAAATG ATGAGTCACA GGAGAACATT
    AAAGATTTGC AGCTGCCTCT CTCCTACTAT TACATTGAGT CTTCACACAA TACCTACCTC
    ACAGGCCACC AGCTCAAAGG AGAGTCATCG GTAGAACTCT ATAGCCAGGT CCTCCTGCAA
    GGCTGTAGAA GTGTAGAGTT GGACTGCTGG GATGGAGATG ACGGGATGCC CATCATCTAT
    CACGGACACA CACTAACAAC CAAGATCCCC TTCAAGGAGG TGGTTGAAGC CATCGACCGC
    AGTGCCTTCA TCAACTCTGA CCTGCCCATC ATCATATCGA TTGAGAACCA CTGCTCGTTG
    CCTCAGCAAC GAAAAATGGC AGAAATTTTC AAGACTGTGT TTGGAGAAAA ACTGGTGGCT
    AAATTCCTGT TTGAGAGCGA TTTCTCAGAT GATCCAATGC TTCCCTCACC TGACCAACTT
    AGGAGGAAAG TGCTTCTTAA AAATAAGAAG CTAAAAGCCC ACCAGACACC AGTGGATATC
    TTAAAGCAAA AGGCTCATCA GTTAGCATCC ATGCAAGCTC AGGCTTATAA TGGGGGAAAT
    GTCAACCCTC CACCTGCTAA TAACGAGGAA GAGGAAGATG AAGAGGACGA ATATGATTAT
    GACTATGAAT CCCTGTCTGA TGCAGATGTC CTTACTGCTT CTCCTGCTCC TAACAGTCAG
    GAAGACAACA TTCTAGAAGA CAGACCTGAA AATAAATCAT GTAATGACAA GCTTCAGTTT
    GAGTATAACG AAGAAATCCC CAAGAGGATA AAGAAAGCAG ATAATTCTGC TTTCAACAAA
    GGAAAGGTTT ATGACATGGA ACTGGGAGAA GAATTTTATC TTCCTCAGAA TAAAAAGGAA
    AGCAGACAGA TTGCACCAGA GCTTTCTGAC CTTGTCATCT ACTGCCAAGC GGTAAAGTTT
    CCGGGGCTGT CAACTCTAAA TGCATCTGGC TCTAACAGAG GAAAAGAGAG GAAAAGCAGG
    AAGTCCATTT TTGGCAACAA TCCGGGCAGA ATGAGCCCTG GGGAGACAGC ATCGTTTAAC
    AAAGCATCTG GAAAAAGTTC CTGTGAAGGA ATGCGACAGG CGTGGGAGGA ATCTTCTTCC
    CCCCTCAACC CAACCACATC CCTCAGCGCT ATCATTAGAA CTCCCAAATG TTACCATATC
    TCATCGCTGA ATGAAAATGC CGCCAAACGT CTGTGTCGCA GGTATTCTCA GAAACTGATC
    CAGCACACTG CCTGTCAGCT GCTGAGGACT TACCCGGCTG CCACACGCAT CGACTCCTCC
    AACCCCAATC CTCTCTTGTT CTGGCTCCAT GGGATACAGC TCGTGGCACT CAACTACCAG
    ACAGATGATC TCCCTTTACA TTTAAATGCT GCCATGTTTG AGGCCAACGG TGGCTGTGGT
    TATGTTTTGA AACCCCCCGT TCTGTGGGAC AAGAACTGTC CCATGTACCA GAAGTTTTCA
    CCCTTGGAAA GAGACCTGGA CAACATGGAG CCTGCCGTCT ATTCCTTAAC TATTGTCTCT
    GGTCAAAACG TGTGCCCAAG TAGCAGCACG GGAAGCCCAT GTATCGAAGT GGACGTGCTG
    GGCATGCCCC TGGACAGTTG CCACTTCCGC ACAAAACCCA TCCACCGGAA CACTTTGAAC
    CCCATGTGGA ATGAACAGTT TCTCTTCCGG GTTCACTTTG AAGATCTCAT ATTCCTGCGT
    TTTGCAGTTG TGGAAAACAA TAGCTCAGCC ATAACCGCTC AGAGAATCAT CCCCCTCAAG
    GCTTTAAAAC GAGGATATCG ACATCTTCAG CTGCGAAACC TCCACAATGA AGTCTTGGAA
    ATCTCTAGTT TATTCATAAA CAGCAGAAGG ATGGAAGAAA ATTCCTCCAG CAGTGCCACG
    CCAGCCTCTT TGATGTTTAA TACAGAAGAA AGAAAATGTT TGCAGACTCA CAGAGTCACA
    GTGCATGGGG TCCCAGGTCC AGAGCCCTTT ACTGTTTTCT CCATAAGTGG AGGCACGAAG
    GCAAAGCAGC TTCTCCAACA GATTCTAACT ACTGAACAAG ACACCAAACC TATTGTCACA
    GACTATTTTT TGATGGAAGA AAAATATTTT ATATCTAAAG AAAAGAATGA ATGCAGGAAA
    CAACCATTTC AGAGAGTCAT CGGTCCAGAA GAAGAGATCA TGCAGATTTT AAGCAGCTGG
    TTTCCAGAGG AAGGATATGT TGGCAGGATT GTCTTAAAAA CCCAGCAGGA AAACCTAGAA
    GAGAGAAGCA TTGTTCAAGA TGAGAAAGAG GTGATCTTGA GCTCGGAGGA GGAGAGCTTC
    TTTGTCCAAG TGCATGACGT TTCTCCAGAG CAACCTCGGA CGGTCATCAA AGCGCCCCGT
    GTCAGCACCG CTCAGGATGT CATTCAACAG ACCTTATGCA AAGCCAAATA TTCCTATAGC
    ATCCTGAGTA ACCCCAACCC GAGTGACTAT GTGCTTCTGG AAGAGGTGGT GAAAGATGCT
    ACCAAGAAGT CCTCCACCCC AAAGTCCTCC CAGCGGGTCC TTTTGGACCA AGAGTGTGTG
    TTTCAAGCCC AGAGCAAGTG GAAAGGTGCT GGAAAATTCA TCCTCAGGCT AAAGGAGCAG
    GTGCAGGCAT CCCGAGAAGA TAAAAGAAAA GGCATTTCTT TCGCAAGTGA ATTCAAGAAG
    TTCACCAAAT CAACTAAACA GCCCCGAGGA CTCACATCTT CTCAGGTCTT GGCCTCAGAA
    AGTGTCCAAA ACAAGGAGGA GAAGCCCACG GGCAGCTTGT CCTCCAGTGA CACCACAGAC
    TCCCGACAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27696
    Clone ID Related Accession (Same CDS sequence) XM_022411587.1 , NM_001136565.1 , XM_005637459.2
    Accession Version XM_022411587.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6930bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006610.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGACTTCTG AAGGAATGGC AGCTTCTGTT CTCACACCTG TGACTCAAAG AAAAGTGACA 
    TTTGCTCCAT CGGCTGTAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAG TGTTCCAAAG
    GCACATAGTG TCAAGCAGAG TGAGCAGACT TCTACCATCC CGTGGATGAA CAAGCTTAAA
    GAAGAATCTT CTGGAAGCAA CTTGCCCAAA ATTCTCTCCA TAGCAAGGGA GAAAATAGCA
    AGTGATGAGA ACAGCAATGA AGAGTGCTGG ACGGAAAGCA CACCAGTTTC TGTGAAAAAC
    CTTAACATTA ACCATAACAA CATCCTGACA AACCGCCAGT GCGTCCTTCC TCAGAGCCAG
    TCTTATGACA CGTGCAATTC TGTCATGGAG GAAGACCCAT GTTTAGAAAC TGGAATCTCA
    TCCTCACTGG AAAGAAAGGT ATTCCCTGGA ATTCAACTGG AAGTCAACAG ACCTCCCATG
    GACTTTAGGC CTCCAGGCCT AATGGACTTT AGTACCTTAG GAAGTCAGTC AGCCATTGTA
    GATACTGGCC AGGCGCACCC TGACAGCAAC AAAGCAGCAT TTCAAATCTT TAATTATAAA
    GTTGACAGAA GGATGTCTGA CACTTTCTGT ACCCTGTCAG GTGACTTGAT ATTGGATGAC
    TGTGGAAACT GTGTGCCACT GTCATCTGGT TTTGGCGGGG AGCAAAAGAA AAATTACGTG
    GCATATACCT GTAAACTCAT GGAATTGGCT GAAAACTGTG ATAATGAGAA TGGACAGCTG
    CAGTGTGATG ATTATGATGC CTTGGATGAC AAATACTTGT GCTTTGAAGA TTCTTGCCAA
    AGGGACAGTG TGGTATGTTC AAGTGACAGC TTTCGCAGGG AGGACCTAAC TAACAGTCCA
    CCTGCCAAGA CCTTTCTGAG CCATTTTGAA GACTTCCCTG ATAATGGTGA AGATGTAGAA
    GATTTTTTAA AAAACAAAAA GGAGAGATCC ACTTTGTTAG TGAGGAGATT TTGTAAAAAT
    GACAGAGAAG TAAAGAAATC TGTGTATACT GGGACAAGGG CAATCATGAG AACTCTGCCT
    TCTGGTCACA TTGGGCTGGA AGCTTACAGT TACATCGATC AGAAGAGAAG TGGTCCCTTA
    TTACCCCGTG GGAGAGTTCT GGAACAGCTG CCAGTGGTGG CAATTAGGCA AGATGGGAGC
    CAGTGCCTGT CAGAAGCCCA GTGGTATCGG ATCTACAATG CAGTGAGAAG GGAAGAAGAA
    ATAGAAAATA CCATTGGATC TCTTCTCCAT TACTTTACCA AGCTCCCAGC CTCCAAGACA
    GCCCATGAAA GGATTAGTGT TGGTCCATGC CTAAAGCAGT GTGTCCGAGA CACCATATGT
    GAGTATCGTG CCACCCTCCA GAGGACGTCC ATATCTCAGT ACATCACGGG TTCGCTCCTA
    GAAGCAACCA CATCTTTAGG AGCGAGAAGT GGCCTACTCA GTACTTTTGG AGGATCCACT
    GGACGAATGA TGCTAAAAGA ACGCCAGCCA GGCACCTCCA TGGCCAATTC CAGTGCCCTC
    CCTTCAAGTT CCGCTGGAAT CAGCAAGGAG CTGATTGACC TGCAGCCTCT CATTCAGTTC
    CCAGAGGAAG TCGCCAGCAT CCTGATGGAG CAGGAACAGA ACATTTACCG AAGGGTCTTA
    CCAGTTGACT ATCTTTACTT CTTGACCCGG GACTTGGGCA CTCCAGAATG CCAGACACCC
    CTGCCCTGCC TCAAAGCATC CATCTCAGCT TCAATTCTTA GCTCCCAGAA CGGAGAGCAC
    AATGCCCTGG AAGATCTTGT GATGAGATTT AATGAGGTGA GCTCCTGGGT GACATGGCTG
    ATCCTCACGG CGGGCTCCAT GGAGGAGAAG CGAGAAGTCT TCTCCTATTT GGTGCATGTG
    GCCAAATGCT GCTGGAACAT GGGCAACTAC AATGCTGTCA TGGAGTTCTT GGCTGGTCTC
    AGGTCAAGAA AAGTTTTAAA AATGTGGCAG TTTATGGACC AATCTGACCT CGAGACCATG
    AGGAGCCTGA AGGACGCCAT GGCTCAGCAT GAATCTTCCT GCGAATATCG GAAGGTGATA
    ACGAGGGCTC TGCACATACC TGGCTGTAAA GTGGTTCCAT TCTGTGGAGT GTTTCTGAAG
    GAGCTCTGTG AAGTACTGGA CGGAGCCTCT GGCCTCATGA AGCTTTGCCC GAGGTACAAT
    TCCCAAGAAG AAACTCTAGA GTTTGTAGCA GATTACAGTG GACAAGATAA TTTCTTACAA
    CGAGTGGGGC ATAATGGCTT AAAGAATTCA GAGAAGGAGT CCACTGTCAA CAGCATCTTT
    CAAATCATTA GGAGCTGCAG CCGAAGTCTG GAGGCTGAGG AAGAGGACAG CCCCAGTGAA
    GGGAACAGCT CTAGGAAAAA TTCTTTAAGG GACAAAGCCC GATGGCAATT TATAATCGGA
    GATTTGTTGG ATTCCGAAAA TGACATCTTT GAGCAATCCA AAGAATGGGA CTCTCCCAGC
    TCAGAAGAGC CTCAGAAAGC CTTTGACCAT GGGACAGAGC TGATCCCTTG GTACGTGCTG
    TCCATCCGAG CAGATGTGCA CCAGTTCCTG CTACAGGGCG CCACCGTCCT CCGCTACGAC
    CAGGACACGC ACCTCTCTGC CCGCTGCTTC CTCCAGCTTC AGCCTGACAA TAGCACCTTG
    ACATGGATAA AGCCCACAGC TGCCTCCCCG GCCAGTGCTA GAGCAAAACT CGGTGTGCTG
    AGCAACACGT CTGAGCCTGG CAAGTTCCCG TCACCAGGCA GTGCCGGACT AAGCGGCCTG
    GCCGAGGGGG TCCTGGATCT GTTTTCAGCA AAGGCTGTGT ACATGGGACA CCCTAGCATT
    GATATCCACA CTGTGTGTGT CCAGAACAAA CTCGGTAGCA TGTTTCTCTC AGAGACTGGC
    GTGACCCTGC TCTACGGGCT CCAGACCACA GACAATAGAT TATTGCACTT TGTGGCACCC
    AAACACACTG CTAAGATGCT CTTCAGTGGA TTGCTAGAAC TCACGAGGGC CGTGAGGAAG
    ATGAGAAAGT TCCCTGACCA GAGGCAGCAG TGGCTGCGGA AACAGTATGT TAGCCTCTAT
    CAGGAGGACG GACGGTATGA GGGCCCAACT TTGGCTCATG CTGTGGAGTT GTTTGGTGGC
    CGGCGATGGA GTACACGCAA TCCCAGCCCT GGGACATCTG CAAAGAGTGC TGAGAAGCCC
    AGTGTGCAGA GAAACAACAC CTTGGGCATA AGCACCACCA AGAAAAAGAA GAAAATCCTT
    ATAAGGGGTG AGAGTGGAGA GGCAGCCGAC GATGAGATGG CCACCCGGAA GGCCAAAATG
    CACAGGGAGT GTCGCAGCCG GAGTGGTTCT GACCCACAAG ACATGAATGA GCAAGAAGAA
    TCTGAGGCAA ATGCCATCAT GAGCCCTCCT AACACTCTGC CTTCCAGAAG AGCCCACTCT
    TTGACTACAG CTGGGTCCCC TAATTTGTCT GCCGGGACAT CATCTCCCAT CAGGCCAGTG
    TCCTCCCCTG TGCTGTCTTC TTCAAACAAG AGCCCGTCCA GTGCTTGGAG CAGCAGCAGC
    TGGCATGGAA GGATCAAAGG AGGAATGAAG GGTTTTCAGA GCTTCATGGT TTCAGACAGT
    AACATGAGTT TTGTTGAATT TGTTGAGCTG TTCAAATCAT TCAGTGTGAG GAGCCGCAAG
    GACCTGAAGG ATCTCTTTGA CATCTACGCT GTGCCCTGCA ATCGGGCCGG CTCTGAGTCG
    GCCCCGCTCT ACACCAACCT AACAATAGAC GAAAACACCA GCGATCTTCA GCCTGACCTA
    GATTTATTGA CCAGAAACGT CTCGGATTTG GGGTTGTTCA TTAAGAGTAA ACAGCAGCTG
    TCAGACAACC AGAGGCAGAT TTCTGATGCC ATTGCTGCTG CAAGTATCGT GACGAATGGC
    ACTGGGATCG AGAGCACATC TTTGGGCATC TTTGGGGTTG GCATACTCCA GCTCAATGAC
    TTCCTAGTCA ACTGCCAGGG AGAACACTGC ACTTACGATG AAATCCTCAG CATCATCCAG
    AAGTTCGAGC CTAGCATTAG TATGTGTCAT CAGGGCCTAC TGTCCTTTGA AGGATTTGCA
    AGGTTTCTGA TGGATAAAGA TAATTTTGCC TCCAAAAATG ATGAGTCACA GGAGAACATT
    AAAGATTTGC AGCTGCCTCT CTCCTACTAT TACATTGAGT CTTCACACAA TACCTACCTC
    ACAGGCCACC AGCTCAAAGG AGAGTCATCG GTAGAACTCT ATAGCCAGGT CCTCCTGCAA
    GGCTGTAGAA GTGTAGAGTT GGACTGCTGG GATGGAGATG ACGGGATGCC CATCATCTAT
    CACGGACACA CACTAACAAC CAAGATCCCC TTCAAGGAGG TGGTTGAAGC CATCGACCGC
    AGTGCCTTCA TCAACTCTGA CCTGCCCATC ATCATATCGA TTGAGAACCA CTGCTCGTTG
    CCTCAGCAAC GAAAAATGGC AGAAATTTTC AAGACTGTGT TTGGAGAAAA ACTGGTGGCT
    AAATTCCTGT TTGAGAGCGA TTTCTCAGAT GATCCAATGC TTCCCTCACC TGACCAACTT
    AGGAGGAAAG TGCTTCTTAA AAATAAGAAG CTAAAAGCCC ACCAGACACC AGTGGATATC
    TTAAAGCAAA AGGCTCATCA GTTAGCATCC ATGCAAGCTC AGGCTTATAA TGGGGGAAAT
    GTCAACCCTC CACCTGCTAA TAACGAGGAA GAGGAAGATG AAGAGGACGA ATATGATTAT
    GACTATGAAT CCCTGTCTGA TGACAACATT CTAGAAGACA GACCTGAAAA TAAATCATGT
    AATGACAAGC TTCAGTTTGA GTATAACGAA GAAATCCCCA AGAGGATAAA GAAAGCAGAT
    AATTCTGCTT TCAACAAAGG AAAGGTTTAT GACATGGAAC TGGGAGAAGA ATTTTATCTT
    CCTCAGAATA AAAAGGAAAG CAGACAGATT GCACCAGAGC TTTCTGACCT TGTCATCTAC
    TGCCAAGCGG TAAAGTTTCC GGGGCTGTCA ACTCTAAATG CATCTGGCTC TAACAGAGGA
    AAAGAGAGGA AAAGCAGGAA GTCCATTTTT GGCAACAATC CGGGCAGAAT GAGCCCTGGG
    GAGACAGCAT CGTTTAACAA AGCATCTGGA AAAAGTTCCT GTGAAGGAAT GCGACAGGCG
    TGGGAGGAAT CTTCTTCCCC CCTCAACCCA ACCACATCCC TCAGCGCTAT CATTAGAACT
    CCCAAATGTT ACCATATCTC ATCGCTGAAT GAAAATGCCG CCAAACGTCT GTGTCGCAGG
    TATTCTCAGA AACTGATCCA GCACACTGCC TGTCAGCTGC TGAGGACTTA CCCGGCTGCC
    ACACGCATCG ACTCCTCCAA CCCCAATCCT CTCTTGTTCT GGCTCCATGG GATACAGCTC
    GTGGCACTCA ACTACCAGAC AGATGATCTC CCTTTACATT TAAATGCTGC CATGTTTGAG
    GCCAACGGTG GCTGTGGTTA TGTTTTGAAA CCCCCCGTTC TGTGGGACAA GAACTGTCCC
    ATGTACCAGA AGTTTTCACC CTTGGAAAGA GACCTGGACA ACATGGAGCC TGCCGTCTAT
    TCCTTAACTA TTGTCTCTGG TCAAAACGTG TGCCCAAGTA GCAGCACGGG AAGCCCATGT
    ATCGAAGTGG ACGTGCTGGG CATGCCCCTG GACAGTTGCC ACTTCCGCAC AAAACCCATC
    CACCGGAACA CTTTGAACCC CATGTGGAAT GAACAGTTTC TCTTCCGGGT TCACTTTGAA
    GATCTCATAT TCCTGCGTTT TGCAGTTGTG GAAAACAATA GCTCAGCCAT AACCGCTCAG
    AGAATCATCC CCCTCAAGGC TTTAAAACGA GGATATCGAC ATCTTCAGCT GCGAAACCTC
    CACAATGAAG TCTTGGAAAT CTCTAGTTTA TTCATAAACA GCAGAAGGAT GGAAGAAAAT
    TCCTCCAGCA GTGCCACGCC AGCCTCTTTG ATGTTTAATA CAGAAGAAAG AAAATGTTTG
    CAGACTCACA GAGTCACAGT GCATGGGGTC CCAGGTCCAG AGCCCTTTAC TGTTTTCTCC
    ATAAGTGGAG GCACGAAGGC AAAGCAGCTT CTCCAACAGA TTCTAACTAC TGAACAAGAC
    ACCAAACCTA TTGTCACAGA CTATTTTTTG ATGGAAGAAA AATATTTTAT ATCTAAAGAA
    AAGAATGAAT GCAGGAAACA ACCATTTCAG AGAGTCATCG GTCCAGAAGA AGAGATCATG
    CAGATTTTAA GCAGCTGGTT TCCAGAGGAA GGATATGTTG GCAGGATTGT CTTAAAAACC
    CAGCAGGAAA ACCTAGAAGA GAGAAGCATT GTTCAAGATG AGAAAGAGGT GATCTTGAGC
    TCGGAGGAGG AGAGCTTCTT TGTCCAAGTG CATGACGTTT CTCCAGAGCA ACCTCGGACG
    GTCATCAAAG CGCCCCGTGT CAGCACCGCT CAGGATGTCA TTCAACAGAC CTTATGCAAA
    GCCAAATATT CCTATAGCAT CCTGAGTAAC CCCAACCCGA GTGACTATGT GCTTCTGGAA
    GAGGTGGTGA AAGATGCTAC CAAGAAGTCC TCCACCCCAA AGTCCTCCCA GCGGGTCCTT
    TTGGACCAAG AGTGTGTGTT TCAAGCCCAG AGCAAGTGGA AAGGTGCTGG AAAATTCATC
    CTCAGGCTAA AGGAGCAGGT GCAGGCATCC CGAGAAGATA AAAGAAAAGG CATTTCTTTC
    GCAAGTGAAT TCAAGAAGTT CACCAAATCA ACTAAACAGC CCCGAGGACT CACATCTTCT
    CAGGTCTTGG CCTCAGAAAG TGTCCAAAAC AAGGAGGAGA AGCCCACGGG CAGCTTGTCC
    TCCAGTGACA CCACAGACTC CCGACAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022267295.1
    CDS352..7281
    Translation

    Target ORF information:

    RefSeq Version XM_022411587.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris phospholipase C epsilon 1 (PLCE1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022411587.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGACTTCTG AAGGAATGGC AGCTTCTGTT CTCACACCTG TGACTCAAAG AAAAGTGACA 
    TTTGCTCCAT CGGCTGTAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAG TGTTCCAAAG
    GCACATAGTG TCAAGCAGAG TGAGCAGACT TCTACCATCC CGTGGATGAA CAAGCTTAAA
    GAAGAATCTT CTGGAAGCAA CTTGCCCAAA ATTCTCTCCA TAGCAAGGGA GAAAATAGCA
    AGTGATGAGA ACAGCAATGA AGAGTGCTGG ACGGAAAGCA CACCAGTTTC TGTGAAAAAC
    CTTAACATTA ACCATAACAA CATCCTGACA AACCGCCAGT GCGTCCTTCC TCAGAGCCAG
    TCTTATGACA CGTGCAATTC TGTCATGGAG GAAGACCCAT GTTTAGAAAC TGGAATCTCA
    TCCTCACTGG AAAGAAAGGT ATTCCCTGGA ATTCAACTGG AAGTCAACAG ACCTCCCATG
    GACTTTAGGC CTCCAGGCCT AATGGACTTT AGTACCTTAG GAAGTCAGTC AGCCATTGTA
    GATACTGGCC AGGCGCACCC TGACAGCAAC AAAGCAGCAT TTCAAATCTT TAATTATAAA
    GTTGACAGAA GGATGTCTGA CACTTTCTGT ACCCTGTCAG GTGACTTGAT ATTGGATGAC
    TGTGGAAACT GTGTGCCACT GTCATCTGGT TTTGGCGGGG AGCAAAAGAA AAATTACGTG
    GCATATACCT GTAAACTCAT GGAATTGGCT GAAAACTGTG ATAATGAGAA TGGACAGCTG
    CAGTGTGATG ATTATGATGC CTTGGATGAC AAATACTTGT GCTTTGAAGA TTCTTGCCAA
    AGGGACAGTG TGGTATGTTC AAGTGACAGC TTTCGCAGGG AGGACCTAAC TAACAGTCCA
    CCTGCCAAGA CCTTTCTGAG CCATTTTGAA GACTTCCCTG ATAATGGTGA AGATGTAGAA
    GATTTTTTAA AAAACAAAAA GGAGAGATCC ACTTTGTTAG TGAGGAGATT TTGTAAAAAT
    GACAGAGAAG TAAAGAAATC TGTGTATACT GGGACAAGGG CAATCATGAG AACTCTGCCT
    TCTGGTCACA TTGGGCTGGA AGCTTACAGT TACATCGATC AGAAGAGAAG TGGTCCCTTA
    TTACCCCGTG GGAGAGTTCT GGAACAGCTG CCAGTGGTGG CAATTAGGCA AGATGGGAGC
    CAGTGCCTGT CAGAAGCCCA GTGGTATCGG ATCTACAATG CAGTGAGAAG GGAAGAAGAA
    ATAGAAAATA CCATTGGATC TCTTCTCCAT TACTTTACCA AGCTCCCAGC CTCCAAGACA
    GCCCATGAAA GGATTAGTGT TGGTCCATGC CTAAAGCAGT GTGTCCGAGA CACCATATGT
    GAGTATCGTG CCACCCTCCA GAGGACGTCC ATATCTCAGT ACATCACGGG TTCGCTCCTA
    GAAGCAACCA CATCTTTAGG AGCGAGAAGT GGCCTACTCA GTACTTTTGG AGGATCCACT
    GGACGAATGA TGCTAAAAGA ACGCCAGCCA GGCACCTCCA TGGCCAATTC CAGTGCCCTC
    CCTTCAAGTT CCGCTGGAAT CAGCAAGGAG CTGATTGACC TGCAGCCTCT CATTCAGTTC
    CCAGAGGAAG TCGCCAGCAT CCTGATGGAG CAGGAACAGA ACATTTACCG AAGGGTCTTA
    CCAGTTGACT ATCTTTACTT CTTGACCCGG GACTTGGGCA CTCCAGAATG CCAGACACCC
    CTGCCCTGCC TCAAAGCATC CATCTCAGCT TCAATTCTTA GCTCCCAGAA CGGAGAGCAC
    AATGCCCTGG AAGATCTTGT GATGAGATTT AATGAGGTGA GCTCCTGGGT GACATGGCTG
    ATCCTCACGG CGGGCTCCAT GGAGGAGAAG CGAGAAGTCT TCTCCTATTT GGTGCATGTG
    GCCAAATGCT GCTGGAACAT GGGCAACTAC AATGCTGTCA TGGAGTTCTT GGCTGGTCTC
    AGGTCAAGAA AAGTTTTAAA AATGTGGCAG TTTATGGACC AATCTGACCT CGAGACCATG
    AGGAGCCTGA AGGACGCCAT GGCTCAGCAT GAATCTTCCT GCGAATATCG GAAGGTGATA
    ACGAGGGCTC TGCACATACC TGGCTGTAAA GTGGTTCCAT TCTGTGGAGT GTTTCTGAAG
    GAGCTCTGTG AAGTACTGGA CGGAGCCTCT GGCCTCATGA AGCTTTGCCC GAGGTACAAT
    TCCCAAGAAG AAACTCTAGA GTTTGTAGCA GATTACAGTG GACAAGATAA TTTCTTACAA
    CGAGTGGGGC ATAATGGCTT AAAGAATTCA GAGAAGGAGT CCACTGTCAA CAGCATCTTT
    CAAATCATTA GGAGCTGCAG CCGAAGTCTG GAGGCTGAGG AAGAGGACAG CCCCAGTGAA
    GGGAACAGCT CTAGGAAAAA TTCTTTAAGG GACAAAGCCC GATGGCAATT TATAATCGGA
    GATTTGTTGG ATTCCGAAAA TGACATCTTT GAGCAATCCA AAGAATGGGA CTCTCCCAGC
    TCAGAAGAGC CTCAGAAAGC CTTTGACCAT GGGACAGAGC TGATCCCTTG GTACGTGCTG
    TCCATCCGAG CAGATGTGCA CCAGTTCCTG CTACAGGGCG CCACCGTCCT CCGCTACGAC
    CAGGACACGC ACCTCTCTGC CCGCTGCTTC CTCCAGCTTC AGCCTGACAA TAGCACCTTG
    ACATGGATAA AGCCCACAGC TGCCTCCCCG GCCAGTGCTA GAGCAAAACT CGGTGTGCTG
    AGCAACACGT CTGAGCCTGG CAAGTTCCCG TCACCAGGCA GTGCCGGACT AAGCGGCCTG
    GCCGAGGGGG TCCTGGATCT GTTTTCAGCA AAGGCTGTGT ACATGGGACA CCCTAGCATT
    GATATCCACA CTGTGTGTGT CCAGAACAAA CTCGGTAGCA TGTTTCTCTC AGAGACTGGC
    GTGACCCTGC TCTACGGGCT CCAGACCACA GACAATAGAT TATTGCACTT TGTGGCACCC
    AAACACACTG CTAAGATGCT CTTCAGTGGA TTGCTAGAAC TCACGAGGGC CGTGAGGAAG
    ATGAGAAAGT TCCCTGACCA GAGGCAGCAG TGGCTGCGGA AACAGTATGT TAGCCTCTAT
    CAGGAGGACG GACGGTATGA GGGCCCAACT TTGGCTCATG CTGTGGAGTT GTTTGGTGGC
    CGGCGATGGA GTACACGCAA TCCCAGCCCT GGGACATCTG CAAAGAGTGC TGAGAAGCCC
    AGTGTGCAGA GAAACAACAC CTTGGGCATA AGCACCACCA AGAAAAAGAA GAAAATCCTT
    ATAAGGGGTG AGAGTGGAGA GGCAGCCGAC GATGAGATGG CCACCCGGAA GGCCAAAATG
    CACAGGGAGT GTCGCAGCCG GAGTGGTTCT GACCCACAAG ACATGAATGA GCAAGAAGAA
    TCTGAGGCAA ATGCCATCAT GAGCCCTCCT AACACTCTGC CTTCCAGAAG AGCCCACTCT
    TTGACTACAG CTGGGTCCCC TAATTTGTCT GCCGGGACAT CATCTCCCAT CAGGCCAGTG
    TCCTCCCCTG TGCTGTCTTC TTCAAACAAG AGCCCGTCCA GTGCTTGGAG CAGCAGCAGC
    TGGCATGGAA GGATCAAAGG AGGAATGAAG GGTTTTCAGA GCTTCATGGT TTCAGACAGT
    AACATGAGTT TTGTTGAATT TGTTGAGCTG TTCAAATCAT TCAGTGTGAG GAGCCGCAAG
    GACCTGAAGG ATCTCTTTGA CATCTACGCT GTGCCCTGCA ATCGGGCCGG CTCTGAGTCG
    GCCCCGCTCT ACACCAACCT AACAATAGAC GAAAACACCA GCGATCTTCA GCCTGACCTA
    GATTTATTGA CCAGAAACGT CTCGGATTTG GGGTTGTTCA TTAAGAGTAA ACAGCAGCTG
    TCAGACAACC AGAGGCAGAT TTCTGATGCC ATTGCTGCTG CAAGTATCGT GACGAATGGC
    ACTGGGATCG AGAGCACATC TTTGGGCATC TTTGGGGTTG GCATACTCCA GCTCAATGAC
    TTCCTAGTCA ACTGCCAGGG AGAACACTGC ACTTACGATG AAATCCTCAG CATCATCCAG
    AAGTTCGAGC CTAGCATTAG TATGTGTCAT CAGGGCCTAC TGTCCTTTGA AGGATTTGCA
    AGGTTTCTGA TGGATAAAGA TAATTTTGCC TCCAAAAATG ATGAGTCACA GGAGAACATT
    AAAGATTTGC AGCTGCCTCT CTCCTACTAT TACATTGAGT CTTCACACAA TACCTACCTC
    ACAGGCCACC AGCTCAAAGG AGAGTCATCG GTAGAACTCT ATAGCCAGGT CCTCCTGCAA
    GGCTGTAGAA GTGTAGAGTT GGACTGCTGG GATGGAGATG ACGGGATGCC CATCATCTAT
    CACGGACACA CACTAACAAC CAAGATCCCC TTCAAGGAGG TGGTTGAAGC CATCGACCGC
    AGTGCCTTCA TCAACTCTGA CCTGCCCATC ATCATATCGA TTGAGAACCA CTGCTCGTTG
    CCTCAGCAAC GAAAAATGGC AGAAATTTTC AAGACTGTGT TTGGAGAAAA ACTGGTGGCT
    AAATTCCTGT TTGAGAGCGA TTTCTCAGAT GATCCAATGC TTCCCTCACC TGACCAACTT
    AGGAGGAAAG TGCTTCTTAA AAATAAGAAG CTAAAAGCCC ACCAGACACC AGTGGATATC
    TTAAAGCAAA AGGCTCATCA GTTAGCATCC ATGCAAGCTC AGGCTTATAA TGGGGGAAAT
    GTCAACCCTC CACCTGCTAA TAACGAGGAA GAGGAAGATG AAGAGGACGA ATATGATTAT
    GACTATGAAT CCCTGTCTGA TGACAACATT CTAGAAGACA GACCTGAAAA TAAATCATGT
    AATGACAAGC TTCAGTTTGA GTATAACGAA GAAATCCCCA AGAGGATAAA GAAAGCAGAT
    AATTCTGCTT TCAACAAAGG AAAGGTTTAT GACATGGAAC TGGGAGAAGA ATTTTATCTT
    CCTCAGAATA AAAAGGAAAG CAGACAGATT GCACCAGAGC TTTCTGACCT TGTCATCTAC
    TGCCAAGCGG TAAAGTTTCC GGGGCTGTCA ACTCTAAATG CATCTGGCTC TAACAGAGGA
    AAAGAGAGGA AAAGCAGGAA GTCCATTTTT GGCAACAATC CGGGCAGAAT GAGCCCTGGG
    GAGACAGCAT CGTTTAACAA AGCATCTGGA AAAAGTTCCT GTGAAGGAAT GCGACAGGCG
    TGGGAGGAAT CTTCTTCCCC CCTCAACCCA ACCACATCCC TCAGCGCTAT CATTAGAACT
    CCCAAATGTT ACCATATCTC ATCGCTGAAT GAAAATGCCG CCAAACGTCT GTGTCGCAGG
    TATTCTCAGA AACTGATCCA GCACACTGCC TGTCAGCTGC TGAGGACTTA CCCGGCTGCC
    ACACGCATCG ACTCCTCCAA CCCCAATCCT CTCTTGTTCT GGCTCCATGG GATACAGCTC
    GTGGCACTCA ACTACCAGAC AGATGATCTC CCTTTACATT TAAATGCTGC CATGTTTGAG
    GCCAACGGTG GCTGTGGTTA TGTTTTGAAA CCCCCCGTTC TGTGGGACAA GAACTGTCCC
    ATGTACCAGA AGTTTTCACC CTTGGAAAGA GACCTGGACA ACATGGAGCC TGCCGTCTAT
    TCCTTAACTA TTGTCTCTGG TCAAAACGTG TGCCCAAGTA GCAGCACGGG AAGCCCATGT
    ATCGAAGTGG ACGTGCTGGG CATGCCCCTG GACAGTTGCC ACTTCCGCAC AAAACCCATC
    CACCGGAACA CTTTGAACCC CATGTGGAAT GAACAGTTTC TCTTCCGGGT TCACTTTGAA
    GATCTCATAT TCCTGCGTTT TGCAGTTGTG GAAAACAATA GCTCAGCCAT AACCGCTCAG
    AGAATCATCC CCCTCAAGGC TTTAAAACGA GGATATCGAC ATCTTCAGCT GCGAAACCTC
    CACAATGAAG TCTTGGAAAT CTCTAGTTTA TTCATAAACA GCAGAAGGAT GGAAGAAAAT
    TCCTCCAGCA GTGCCACGCC AGCCTCTTTG ATGTTTAATA CAGAAGAAAG AAAATGTTTG
    CAGACTCACA GAGTCACAGT GCATGGGGTC CCAGGTCCAG AGCCCTTTAC TGTTTTCTCC
    ATAAGTGGAG GCACGAAGGC AAAGCAGCTT CTCCAACAGA TTCTAACTAC TGAACAAGAC
    ACCAAACCTA TTGTCACAGA CTATTTTTTG ATGGAAGAAA AATATTTTAT ATCTAAAGAA
    AAGAATGAAT GCAGGAAACA ACCATTTCAG AGAGTCATCG GTCCAGAAGA AGAGATCATG
    CAGATTTTAA GCAGCTGGTT TCCAGAGGAA GGATATGTTG GCAGGATTGT CTTAAAAACC
    CAGCAGGAAA ACCTAGAAGA GAGAAGCATT GTTCAAGATG AGAAAGAGGT GATCTTGAGC
    TCGGAGGAGG AGAGCTTCTT TGTCCAAGTG CATGACGTTT CTCCAGAGCA ACCTCGGACG
    GTCATCAAAG CGCCCCGTGT CAGCACCGCT CAGGATGTCA TTCAACAGAC CTTATGCAAA
    GCCAAATATT CCTATAGCAT CCTGAGTAAC CCCAACCCGA GTGACTATGT GCTTCTGGAA
    GAGGTGGTGA AAGATGCTAC CAAGAAGTCC TCCACCCCAA AGTCCTCCCA GCGGGTCCTT
    TTGGACCAAG AGTGTGTGTT TCAAGCCCAG AGCAAGTGGA AAGGTGCTGG AAAATTCATC
    CTCAGGCTAA AGGAGCAGGT GCAGGCATCC CGAGAAGATA AAAGAAAAGG CATTTCTTTC
    GCAAGTGAAT TCAAGAAGTT CACCAAATCA ACTAAACAGC CCCGAGGACT CACATCTTCT
    CAGGTCTTGG CCTCAGAAAG TGTCCAAAAC AAGGAGGAGA AGCCCACGGG CAGCTTGTCC
    TCCAGTGACA CCACAGACTC CCGACAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27696
    Clone ID Related Accession (Same CDS sequence) XM_022411587.1 , NM_001136565.1 , XM_005637459.2
    Accession Version XM_005637459.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6930bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726109.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005637459.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGACTTCTG AAGGAATGGC AGCTTCTGTT CTCACACCTG TGACTCAAAG AAAAGTGACA 
    TTTGCTCCAT CGGCTGTAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAG TGTTCCAAAG
    GCACATAGTG TCAAGCAGAG TGAGCAGACT TCTACCATCC CGTGGATGAA CAAGCTTAAA
    GAAGAATCTT CTGGAAGCAA CTTGCCCAAA ATTCTCTCCA TAGCAAGGGA GAAAATAGCA
    AGTGATGAGA ACAGCAATGA AGAGTGCTGG ACGGAAAGCA CACCAGTTTC TGTGAAAAAC
    CTTAACATTA ACCATAACAA CATCCTGACA AACCGCCAGT GCGTCCTTCC TCAGAGCCAG
    TCTTATGACA CGTGCAATTC TGTCATGGAG GAAGACCCAT GTTTAGAAAC TGGAATCTCA
    TCCTCACTGG AAAGAAAGGT ATTCCCTGGA ATTCAACTGG AAGTCAACAG ACCTCCCATG
    GACTTTAGGC CTCCAGGCCT AATGGACTTT AGTACCTTAG GAAGTCAGTC AGCCATTGTA
    GATACTGGCC AGGCGCACCC TGACAGCAAC AAAGCAGCAT TTCAAATCTT TAATTATAAA
    GTTGACAGAA GGATGTCTGA CACTTTCTGT ACCCTGTCAG GTGACTTGAT ATTGGATGAC
    TGTGGAAACT GTGTGCCACT GTCATCTGGT TTTGGCGGGG AGCAAAAGAA AAATTACGTG
    GCATATACCT GTAAACTCAT GGAATTGGCT GAAAACTGTG ATAATGAGAA TGGACAGCTG
    CAGTGTGATG ATTATGATGC CTTGGATGAC AAATACTTGT GCTTTGAAGA TTCTTGCCAA
    AGGGACAGTG TGGTATGTTC AAGTGACAGC TTTCGCAGGG AGGACCTAAC TAACAGTCCA
    CCTGCCAAGA CCTTTCTGAG CCATTTTGAA GACTTCCCTG ATAATGGTGA AGATGTAGAA
    GATTTTTTAA AAAACAAAAA GGAGAGATCC ACTTTGTTAG TGAGGAGATT TTGTAAAAAT
    GACAGAGAAG TAAAGAAATC TGTGTATACT GGGACAAGGG CAATCATGAG AACTCTGCCT
    TCTGGTCACA TTGGGCTGGA AGCTTACAGT TACATCGATC AGAAGAGAAG TGGTCCCTTA
    TTACCCCGTG GGAGAGTTCT GGAACAGCTG CCAGTGGTGG CAATTAGGCA AGATGGGAGC
    CAGTGCCTGT CAGAAGCCCA GTGGTATCGG ATCTACAATG CAGTGAGAAG GGAAGAAGAA
    ATAGAAAATA CCATTGGATC TCTTCTCCAT TACTTTACCA AGCTCCCAGC CTCCAAGACA
    GCCCATGAAA GGATTAGTGT TGGTCCATGC CTAAAGCAGT GTGTCCGAGA CACCATATGT
    GAGTATCGTG CCACCCTCCA GAGGACGTCC ATATCTCAGT ACATCACGGG TTCGCTCCTA
    GAAGCAACCA CATCTTTAGG AGCGAGAAGT GGCCTACTCA GTACTTTTGG AGGATCCACT
    GGACGAATGA TGCTAAAAGA ACGCCAGCCA GGCACCTCCA TGGCCAATTC CAGTGCCCTC
    CCTTCAAGTT CCGCTGGAAT CAGCAAGGAG CTGATTGACC TGCAGCCTCT CATTCAGTTC
    CCAGAGGAAG TCGCCAGCAT CCTGATGGAG CAGGAACAGA ACATTTACCG AAGGGTCTTA
    CCAGTTGACT ATCTTTACTT CTTGACCCGG GACTTGGGCA CTCCAGAATG CCAGACACCC
    CTGCCCTGCC TCAAAGCATC CATCTCAGCT TCAATTCTTA GCTCCCAGAA CGGAGAGCAC
    AATGCCCTGG AAGATCTTGT GATGAGATTT AATGAGGTGA GCTCCTGGGT GACATGGCTG
    ATCCTCACGG CGGGCTCCAT GGAGGAGAAG CGAGAAGTCT TCTCCTATTT GGTGCATGTG
    GCCAAATGCT GCTGGAACAT GGGCAACTAC AATGCTGTCA TGGAGTTCTT GGCTGGTCTC
    AGGTCAAGAA AAGTTTTAAA AATGTGGCAG TTTATGGACC AATCTGACCT CGAGACCATG
    AGGAGCCTGA AGGACGCCAT GGCTCAGCAT GAATCTTCCT GCGAATATCG GAAGGTGATA
    ACGAGGGCTC TGCACATACC TGGCTGTAAA GTGGTTCCAT TCTGTGGAGT GTTTCTGAAG
    GAGCTCTGTG AAGTACTGGA CGGAGCCTCT GGCCTCATGA AGCTTTGCCC GAGGTACAAT
    TCCCAAGAAG AAACTCTAGA GTTTGTAGCA GATTACAGTG GACAAGATAA TTTCTTACAA
    CGAGTGGGGC ATAATGGCTT AAAGAATTCA GAGAAGGAGT CCACTGTCAA CAGCATCTTT
    CAAATCATTA GGAGCTGCAG CCGAAGTCTG GAGGCTGAGG AAGAGGACAG CCCCAGTGAA
    GGGAACAGCT CTAGGAAAAA TTCTTTAAGG GACAAAGCCC GATGGCAATT TATAATCGGA
    GATTTGTTGG ATTCCGAAAA TGACATCTTT GAGCAATCCA AAGAATGGGA CTCTCCCAGC
    TCAGAAGAGC CTCAGAAAGC CTTTGACCAT GGGACAGAGC TGATCCCTTG GTACGTGCTG
    TCCATCCGAG CAGATGTGCA CCAGTTCCTG CTACAGGGCG CCACCGTCCT CCGCTACGAC
    CAGGACACGC ACCTCTCTGC CCGCTGCTTC CTCCAGCTTC AGCCTGACAA TAGCACCTTG
    ACATGGATAA AGCCCACAGC TGCCTCCCCG GCCAGTGCTA GAGCAAAACT CGGTGTGCTG
    AGCAACACGT CTGAGCCTGG CAAGTTCCCG TCACCAGGCA GTGCCGGACT AAGCGGCCTG
    GCCGAGGGGG TCCTGGATCT GTTTTCAGCA AAGGCTGTGT ACATGGGACA CCCTAGCATT
    GATATCCACA CTGTGTGTGT CCAGAACAAA CTCGGTAGCA TGTTTCTCTC AGAGACTGGC
    GTGACCCTGC TCTACGGGCT CCAGACCACA GACAATAGAT TATTGCACTT TGTGGCACCC
    AAACACACTG CTAAGATGCT CTTCAGTGGA TTGCTAGAAC TCACGAGGGC CGTGAGGAAG
    ATGAGAAAGT TCCCTGACCA GAGGCAGCAG TGGCTGCGGA AACAGTATGT TAGCCTCTAT
    CAGGAGGACG GACGGTATGA GGGCCCAACT TTGGCTCATG CTGTGGAGTT GTTTGGTGGC
    CGGCGATGGA GTACACGCAA TCCCAGCCCT GGGACATCTG CAAAGAGTGC TGAGAAGCCC
    AGTGTGCAGA GAAACAACAC CTTGGGCATA AGCACCACCA AGAAAAAGAA GAAAATCCTT
    ATAAGGGGTG AGAGTGGAGA GGCAGCCGAC GATGAGATGG CCACCCGGAA GGCCAAAATG
    CACAGGGAGT GTCGCAGCCG GAGTGGTTCT GACCCACAAG ACATGAATGA GCAAGAAGAA
    TCTGAGGCAA ATGCCATCAT GAGCCCTCCT AACACTCTGC CTTCCAGAAG AGCCCACTCT
    TTGACTACAG CTGGGTCCCC TAATTTGTCT GCCGGGACAT CATCTCCCAT CAGGCCAGTG
    TCCTCCCCTG TGCTGTCTTC TTCAAACAAG AGCCCGTCCA GTGCTTGGAG CAGCAGCAGC
    TGGCATGGAA GGATCAAAGG AGGAATGAAG GGTTTTCAGA GCTTCATGGT TTCAGACAGT
    AACATGAGTT TTGTTGAATT TGTTGAGCTG TTCAAATCAT TCAGTGTGAG GAGCCGCAAG
    GACCTGAAGG ATCTCTTTGA CATCTACGCT GTGCCCTGCA ATCGGGCCGG CTCTGAGTCG
    GCCCCGCTCT ACACCAACCT AACAATAGAC GAAAACACCA GCGATCTTCA GCCTGACCTA
    GATTTATTGA CCAGAAACGT CTCGGATTTG GGGTTGTTCA TTAAGAGTAA ACAGCAGCTG
    TCAGACAACC AGAGGCAGAT TTCTGATGCC ATTGCTGCTG CAAGTATCGT GACGAATGGC
    ACTGGGATCG AGAGCACATC TTTGGGCATC TTTGGGGTTG GCATACTCCA GCTCAATGAC
    TTCCTAGTCA ACTGCCAGGG AGAACACTGC ACTTACGATG AAATCCTCAG CATCATCCAG
    AAGTTCGAGC CTAGCATTAG TATGTGTCAT CAGGGCCTAC TGTCCTTTGA AGGATTTGCA
    AGGTTTCTGA TGGATAAAGA TAATTTTGCC TCCAAAAATG ATGAGTCACA GGAGAACATT
    AAAGATTTGC AGCTGCCTCT CTCCTACTAT TACATTGAGT CTTCACACAA TACCTACCTC
    ACAGGCCACC AGCTCAAAGG AGAGTCATCG GTAGAACTCT ATAGCCAGGT CCTCCTGCAA
    GGCTGTAGAA GTGTAGAGTT GGACTGCTGG GATGGAGATG ACGGGATGCC CATCATCTAT
    CACGGACACA CACTAACAAC CAAGATCCCC TTCAAGGAGG TGGTTGAAGC CATCGACCGC
    AGTGCCTTCA TCAACTCTGA CCTGCCCATC ATCATATCGA TTGAGAACCA CTGCTCGTTG
    CCTCAGCAAC GAAAAATGGC AGAAATTTTC AAGACTGTGT TTGGAGAAAA ACTGGTGGCT
    AAATTCCTGT TTGAGAGCGA TTTCTCAGAT GATCCAATGC TTCCCTCACC TGACCAACTT
    AGGAGGAAAG TGCTTCTTAA AAATAAGAAG CTAAAAGCCC ACCAGACACC AGTGGATATC
    TTAAAGCAAA AGGCTCATCA GTTAGCATCC ATGCAAGCTC AGGCTTATAA TGGGGGAAAT
    GTCAACCCTC CACCTGCTAA TAACGAGGAA GAGGAAGATG AAGAGGACGA ATATGATTAT
    GACTATGAAT CCCTGTCTGA TGACAACATT CTAGAAGACA GACCTGAAAA TAAATCATGT
    AATGACAAGC TTCAGTTTGA GTATAACGAA GAAATCCCCA AGAGGATAAA GAAAGCAGAT
    AATTCTGCTT TCAACAAAGG AAAGGTTTAT GACATGGAAC TGGGAGAAGA ATTTTATCTT
    CCTCAGAATA AAAAGGAAAG CAGACAGATT GCACCAGAGC TTTCTGACCT TGTCATCTAC
    TGCCAAGCGG TAAAGTTTCC GGGGCTGTCA ACTCTAAATG CATCTGGCTC TAACAGAGGA
    AAAGAGAGGA AAAGCAGGAA GTCCATTTTT GGCAACAATC CGGGCAGAAT GAGCCCTGGG
    GAGACAGCAT CGTTTAACAA AGCATCTGGA AAAAGTTCCT GTGAAGGAAT GCGACAGGCG
    TGGGAGGAAT CTTCTTCCCC CCTCAACCCA ACCACATCCC TCAGCGCTAT CATTAGAACT
    CCCAAATGTT ACCATATCTC ATCGCTGAAT GAAAATGCCG CCAAACGTCT GTGTCGCAGG
    TATTCTCAGA AACTGATCCA GCACACTGCC TGTCAGCTGC TGAGGACTTA CCCGGCTGCC
    ACACGCATCG ACTCCTCCAA CCCCAATCCT CTCTTGTTCT GGCTCCATGG GATACAGCTC
    GTGGCACTCA ACTACCAGAC AGATGATCTC CCTTTACATT TAAATGCTGC CATGTTTGAG
    GCCAACGGTG GCTGTGGTTA TGTTTTGAAA CCCCCCGTTC TGTGGGACAA GAACTGTCCC
    ATGTACCAGA AGTTTTCACC CTTGGAAAGA GACCTGGACA ACATGGAGCC TGCCGTCTAT
    TCCTTAACTA TTGTCTCTGG TCAAAACGTG TGCCCAAGTA GCAGCACGGG AAGCCCATGT
    ATCGAAGTGG ACGTGCTGGG CATGCCCCTG GACAGTTGCC ACTTCCGCAC AAAACCCATC
    CACCGGAACA CTTTGAACCC CATGTGGAAT GAACAGTTTC TCTTCCGGGT TCACTTTGAA
    GATCTCATAT TCCTGCGTTT TGCAGTTGTG GAAAACAATA GCTCAGCCAT AACCGCTCAG
    AGAATCATCC CCCTCAAGGC TTTAAAACGA GGATATCGAC ATCTTCAGCT GCGAAACCTC
    CACAATGAAG TCTTGGAAAT CTCTAGTTTA TTCATAAACA GCAGAAGGAT GGAAGAAAAT
    TCCTCCAGCA GTGCCACGCC AGCCTCTTTG ATGTTTAATA CAGAAGAAAG AAAATGTTTG
    CAGACTCACA GAGTCACAGT GCATGGGGTC CCAGGTCCAG AGCCCTTTAC TGTTTTCTCC
    ATAAGTGGAG GCACGAAGGC AAAGCAGCTT CTCCAACAGA TTCTAACTAC TGAACAAGAC
    ACCAAACCTA TTGTCACAGA CTATTTTTTG ATGGAAGAAA AATATTTTAT ATCTAAAGAA
    AAGAATGAAT GCAGGAAACA ACCATTTCAG AGAGTCATCG GTCCAGAAGA AGAGATCATG
    CAGATTTTAA GCAGCTGGTT TCCAGAGGAA GGATATGTTG GCAGGATTGT CTTAAAAACC
    CAGCAGGAAA ACCTAGAAGA GAGAAGCATT GTTCAAGATG AGAAAGAGGT GATCTTGAGC
    TCGGAGGAGG AGAGCTTCTT TGTCCAAGTG CATGACGTTT CTCCAGAGCA ACCTCGGACG
    GTCATCAAAG CGCCCCGTGT CAGCACCGCT CAGGATGTCA TTCAACAGAC CTTATGCAAA
    GCCAAATATT CCTATAGCAT CCTGAGTAAC CCCAACCCGA GTGACTATGT GCTTCTGGAA
    GAGGTGGTGA AAGATGCTAC CAAGAAGTCC TCCACCCCAA AGTCCTCCCA GCGGGTCCTT
    TTGGACCAAG AGTGTGTGTT TCAAGCCCAG AGCAAGTGGA AAGGTGCTGG AAAATTCATC
    CTCAGGCTAA AGGAGCAGGT GCAGGCATCC CGAGAAGATA AAAGAAAAGG CATTTCTTTC
    GCAAGTGAAT TCAAGAAGTT CACCAAATCA ACTAAACAGC CCCGAGGACT CACATCTTCT
    CAGGTCTTGG CCTCAGAAAG TGTCCAAAAC AAGGAGGAGA AGCCCACGGG CAGCTTGTCC
    TCCAGTGACA CCACAGACTC CCGACAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005637516.1
    CDS555..7484
    Translation

    Target ORF information:

    RefSeq Version XM_005637459.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris phospholipase C, epsilon 1 (PLCE1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005637459.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGACTTCTG AAGGAATGGC AGCTTCTGTT CTCACACCTG TGACTCAAAG AAAAGTGACA 
    TTTGCTCCAT CGGCTGTAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAG TGTTCCAAAG
    GCACATAGTG TCAAGCAGAG TGAGCAGACT TCTACCATCC CGTGGATGAA CAAGCTTAAA
    GAAGAATCTT CTGGAAGCAA CTTGCCCAAA ATTCTCTCCA TAGCAAGGGA GAAAATAGCA
    AGTGATGAGA ACAGCAATGA AGAGTGCTGG ACGGAAAGCA CACCAGTTTC TGTGAAAAAC
    CTTAACATTA ACCATAACAA CATCCTGACA AACCGCCAGT GCGTCCTTCC TCAGAGCCAG
    TCTTATGACA CGTGCAATTC TGTCATGGAG GAAGACCCAT GTTTAGAAAC TGGAATCTCA
    TCCTCACTGG AAAGAAAGGT ATTCCCTGGA ATTCAACTGG AAGTCAACAG ACCTCCCATG
    GACTTTAGGC CTCCAGGCCT AATGGACTTT AGTACCTTAG GAAGTCAGTC AGCCATTGTA
    GATACTGGCC AGGCGCACCC TGACAGCAAC AAAGCAGCAT TTCAAATCTT TAATTATAAA
    GTTGACAGAA GGATGTCTGA CACTTTCTGT ACCCTGTCAG GTGACTTGAT ATTGGATGAC
    TGTGGAAACT GTGTGCCACT GTCATCTGGT TTTGGCGGGG AGCAAAAGAA AAATTACGTG
    GCATATACCT GTAAACTCAT GGAATTGGCT GAAAACTGTG ATAATGAGAA TGGACAGCTG
    CAGTGTGATG ATTATGATGC CTTGGATGAC AAATACTTGT GCTTTGAAGA TTCTTGCCAA
    AGGGACAGTG TGGTATGTTC AAGTGACAGC TTTCGCAGGG AGGACCTAAC TAACAGTCCA
    CCTGCCAAGA CCTTTCTGAG CCATTTTGAA GACTTCCCTG ATAATGGTGA AGATGTAGAA
    GATTTTTTAA AAAACAAAAA GGAGAGATCC ACTTTGTTAG TGAGGAGATT TTGTAAAAAT
    GACAGAGAAG TAAAGAAATC TGTGTATACT GGGACAAGGG CAATCATGAG AACTCTGCCT
    TCTGGTCACA TTGGGCTGGA AGCTTACAGT TACATCGATC AGAAGAGAAG TGGTCCCTTA
    TTACCCCGTG GGAGAGTTCT GGAACAGCTG CCAGTGGTGG CAATTAGGCA AGATGGGAGC
    CAGTGCCTGT CAGAAGCCCA GTGGTATCGG ATCTACAATG CAGTGAGAAG GGAAGAAGAA
    ATAGAAAATA CCATTGGATC TCTTCTCCAT TACTTTACCA AGCTCCCAGC CTCCAAGACA
    GCCCATGAAA GGATTAGTGT TGGTCCATGC CTAAAGCAGT GTGTCCGAGA CACCATATGT
    GAGTATCGTG CCACCCTCCA GAGGACGTCC ATATCTCAGT ACATCACGGG TTCGCTCCTA
    GAAGCAACCA CATCTTTAGG AGCGAGAAGT GGCCTACTCA GTACTTTTGG AGGATCCACT
    GGACGAATGA TGCTAAAAGA ACGCCAGCCA GGCACCTCCA TGGCCAATTC CAGTGCCCTC
    CCTTCAAGTT CCGCTGGAAT CAGCAAGGAG CTGATTGACC TGCAGCCTCT CATTCAGTTC
    CCAGAGGAAG TCGCCAGCAT CCTGATGGAG CAGGAACAGA ACATTTACCG AAGGGTCTTA
    CCAGTTGACT ATCTTTACTT CTTGACCCGG GACTTGGGCA CTCCAGAATG CCAGACACCC
    CTGCCCTGCC TCAAAGCATC CATCTCAGCT TCAATTCTTA GCTCCCAGAA CGGAGAGCAC
    AATGCCCTGG AAGATCTTGT GATGAGATTT AATGAGGTGA GCTCCTGGGT GACATGGCTG
    ATCCTCACGG CGGGCTCCAT GGAGGAGAAG CGAGAAGTCT TCTCCTATTT GGTGCATGTG
    GCCAAATGCT GCTGGAACAT GGGCAACTAC AATGCTGTCA TGGAGTTCTT GGCTGGTCTC
    AGGTCAAGAA AAGTTTTAAA AATGTGGCAG TTTATGGACC AATCTGACCT CGAGACCATG
    AGGAGCCTGA AGGACGCCAT GGCTCAGCAT GAATCTTCCT GCGAATATCG GAAGGTGATA
    ACGAGGGCTC TGCACATACC TGGCTGTAAA GTGGTTCCAT TCTGTGGAGT GTTTCTGAAG
    GAGCTCTGTG AAGTACTGGA CGGAGCCTCT GGCCTCATGA AGCTTTGCCC GAGGTACAAT
    TCCCAAGAAG AAACTCTAGA GTTTGTAGCA GATTACAGTG GACAAGATAA TTTCTTACAA
    CGAGTGGGGC ATAATGGCTT AAAGAATTCA GAGAAGGAGT CCACTGTCAA CAGCATCTTT
    CAAATCATTA GGAGCTGCAG CCGAAGTCTG GAGGCTGAGG AAGAGGACAG CCCCAGTGAA
    GGGAACAGCT CTAGGAAAAA TTCTTTAAGG GACAAAGCCC GATGGCAATT TATAATCGGA
    GATTTGTTGG ATTCCGAAAA TGACATCTTT GAGCAATCCA AAGAATGGGA CTCTCCCAGC
    TCAGAAGAGC CTCAGAAAGC CTTTGACCAT GGGACAGAGC TGATCCCTTG GTACGTGCTG
    TCCATCCGAG CAGATGTGCA CCAGTTCCTG CTACAGGGCG CCACCGTCCT CCGCTACGAC
    CAGGACACGC ACCTCTCTGC CCGCTGCTTC CTCCAGCTTC AGCCTGACAA TAGCACCTTG
    ACATGGATAA AGCCCACAGC TGCCTCCCCG GCCAGTGCTA GAGCAAAACT CGGTGTGCTG
    AGCAACACGT CTGAGCCTGG CAAGTTCCCG TCACCAGGCA GTGCCGGACT AAGCGGCCTG
    GCCGAGGGGG TCCTGGATCT GTTTTCAGCA AAGGCTGTGT ACATGGGACA CCCTAGCATT
    GATATCCACA CTGTGTGTGT CCAGAACAAA CTCGGTAGCA TGTTTCTCTC AGAGACTGGC
    GTGACCCTGC TCTACGGGCT CCAGACCACA GACAATAGAT TATTGCACTT TGTGGCACCC
    AAACACACTG CTAAGATGCT CTTCAGTGGA TTGCTAGAAC TCACGAGGGC CGTGAGGAAG
    ATGAGAAAGT TCCCTGACCA GAGGCAGCAG TGGCTGCGGA AACAGTATGT TAGCCTCTAT
    CAGGAGGACG GACGGTATGA GGGCCCAACT TTGGCTCATG CTGTGGAGTT GTTTGGTGGC
    CGGCGATGGA GTACACGCAA TCCCAGCCCT GGGACATCTG CAAAGAGTGC TGAGAAGCCC
    AGTGTGCAGA GAAACAACAC CTTGGGCATA AGCACCACCA AGAAAAAGAA GAAAATCCTT
    ATAAGGGGTG AGAGTGGAGA GGCAGCCGAC GATGAGATGG CCACCCGGAA GGCCAAAATG
    CACAGGGAGT GTCGCAGCCG GAGTGGTTCT GACCCACAAG ACATGAATGA GCAAGAAGAA
    TCTGAGGCAA ATGCCATCAT GAGCCCTCCT AACACTCTGC CTTCCAGAAG AGCCCACTCT
    TTGACTACAG CTGGGTCCCC TAATTTGTCT GCCGGGACAT CATCTCCCAT CAGGCCAGTG
    TCCTCCCCTG TGCTGTCTTC TTCAAACAAG AGCCCGTCCA GTGCTTGGAG CAGCAGCAGC
    TGGCATGGAA GGATCAAAGG AGGAATGAAG GGTTTTCAGA GCTTCATGGT TTCAGACAGT
    AACATGAGTT TTGTTGAATT TGTTGAGCTG TTCAAATCAT TCAGTGTGAG GAGCCGCAAG
    GACCTGAAGG ATCTCTTTGA CATCTACGCT GTGCCCTGCA ATCGGGCCGG CTCTGAGTCG
    GCCCCGCTCT ACACCAACCT AACAATAGAC GAAAACACCA GCGATCTTCA GCCTGACCTA
    GATTTATTGA CCAGAAACGT CTCGGATTTG GGGTTGTTCA TTAAGAGTAA ACAGCAGCTG
    TCAGACAACC AGAGGCAGAT TTCTGATGCC ATTGCTGCTG CAAGTATCGT GACGAATGGC
    ACTGGGATCG AGAGCACATC TTTGGGCATC TTTGGGGTTG GCATACTCCA GCTCAATGAC
    TTCCTAGTCA ACTGCCAGGG AGAACACTGC ACTTACGATG AAATCCTCAG CATCATCCAG
    AAGTTCGAGC CTAGCATTAG TATGTGTCAT CAGGGCCTAC TGTCCTTTGA AGGATTTGCA
    AGGTTTCTGA TGGATAAAGA TAATTTTGCC TCCAAAAATG ATGAGTCACA GGAGAACATT
    AAAGATTTGC AGCTGCCTCT CTCCTACTAT TACATTGAGT CTTCACACAA TACCTACCTC
    ACAGGCCACC AGCTCAAAGG AGAGTCATCG GTAGAACTCT ATAGCCAGGT CCTCCTGCAA
    GGCTGTAGAA GTGTAGAGTT GGACTGCTGG GATGGAGATG ACGGGATGCC CATCATCTAT
    CACGGACACA CACTAACAAC CAAGATCCCC TTCAAGGAGG TGGTTGAAGC CATCGACCGC
    AGTGCCTTCA TCAACTCTGA CCTGCCCATC ATCATATCGA TTGAGAACCA CTGCTCGTTG
    CCTCAGCAAC GAAAAATGGC AGAAATTTTC AAGACTGTGT TTGGAGAAAA ACTGGTGGCT
    AAATTCCTGT TTGAGAGCGA TTTCTCAGAT GATCCAATGC TTCCCTCACC TGACCAACTT
    AGGAGGAAAG TGCTTCTTAA AAATAAGAAG CTAAAAGCCC ACCAGACACC AGTGGATATC
    TTAAAGCAAA AGGCTCATCA GTTAGCATCC ATGCAAGCTC AGGCTTATAA TGGGGGAAAT
    GTCAACCCTC CACCTGCTAA TAACGAGGAA GAGGAAGATG AAGAGGACGA ATATGATTAT
    GACTATGAAT CCCTGTCTGA TGACAACATT CTAGAAGACA GACCTGAAAA TAAATCATGT
    AATGACAAGC TTCAGTTTGA GTATAACGAA GAAATCCCCA AGAGGATAAA GAAAGCAGAT
    AATTCTGCTT TCAACAAAGG AAAGGTTTAT GACATGGAAC TGGGAGAAGA ATTTTATCTT
    CCTCAGAATA AAAAGGAAAG CAGACAGATT GCACCAGAGC TTTCTGACCT TGTCATCTAC
    TGCCAAGCGG TAAAGTTTCC GGGGCTGTCA ACTCTAAATG CATCTGGCTC TAACAGAGGA
    AAAGAGAGGA AAAGCAGGAA GTCCATTTTT GGCAACAATC CGGGCAGAAT GAGCCCTGGG
    GAGACAGCAT CGTTTAACAA AGCATCTGGA AAAAGTTCCT GTGAAGGAAT GCGACAGGCG
    TGGGAGGAAT CTTCTTCCCC CCTCAACCCA ACCACATCCC TCAGCGCTAT CATTAGAACT
    CCCAAATGTT ACCATATCTC ATCGCTGAAT GAAAATGCCG CCAAACGTCT GTGTCGCAGG
    TATTCTCAGA AACTGATCCA GCACACTGCC TGTCAGCTGC TGAGGACTTA CCCGGCTGCC
    ACACGCATCG ACTCCTCCAA CCCCAATCCT CTCTTGTTCT GGCTCCATGG GATACAGCTC
    GTGGCACTCA ACTACCAGAC AGATGATCTC CCTTTACATT TAAATGCTGC CATGTTTGAG
    GCCAACGGTG GCTGTGGTTA TGTTTTGAAA CCCCCCGTTC TGTGGGACAA GAACTGTCCC
    ATGTACCAGA AGTTTTCACC CTTGGAAAGA GACCTGGACA ACATGGAGCC TGCCGTCTAT
    TCCTTAACTA TTGTCTCTGG TCAAAACGTG TGCCCAAGTA GCAGCACGGG AAGCCCATGT
    ATCGAAGTGG ACGTGCTGGG CATGCCCCTG GACAGTTGCC ACTTCCGCAC AAAACCCATC
    CACCGGAACA CTTTGAACCC CATGTGGAAT GAACAGTTTC TCTTCCGGGT TCACTTTGAA
    GATCTCATAT TCCTGCGTTT TGCAGTTGTG GAAAACAATA GCTCAGCCAT AACCGCTCAG
    AGAATCATCC CCCTCAAGGC TTTAAAACGA GGATATCGAC ATCTTCAGCT GCGAAACCTC
    CACAATGAAG TCTTGGAAAT CTCTAGTTTA TTCATAAACA GCAGAAGGAT GGAAGAAAAT
    TCCTCCAGCA GTGCCACGCC AGCCTCTTTG ATGTTTAATA CAGAAGAAAG AAAATGTTTG
    CAGACTCACA GAGTCACAGT GCATGGGGTC CCAGGTCCAG AGCCCTTTAC TGTTTTCTCC
    ATAAGTGGAG GCACGAAGGC AAAGCAGCTT CTCCAACAGA TTCTAACTAC TGAACAAGAC
    ACCAAACCTA TTGTCACAGA CTATTTTTTG ATGGAAGAAA AATATTTTAT ATCTAAAGAA
    AAGAATGAAT GCAGGAAACA ACCATTTCAG AGAGTCATCG GTCCAGAAGA AGAGATCATG
    CAGATTTTAA GCAGCTGGTT TCCAGAGGAA GGATATGTTG GCAGGATTGT CTTAAAAACC
    CAGCAGGAAA ACCTAGAAGA GAGAAGCATT GTTCAAGATG AGAAAGAGGT GATCTTGAGC
    TCGGAGGAGG AGAGCTTCTT TGTCCAAGTG CATGACGTTT CTCCAGAGCA ACCTCGGACG
    GTCATCAAAG CGCCCCGTGT CAGCACCGCT CAGGATGTCA TTCAACAGAC CTTATGCAAA
    GCCAAATATT CCTATAGCAT CCTGAGTAAC CCCAACCCGA GTGACTATGT GCTTCTGGAA
    GAGGTGGTGA AAGATGCTAC CAAGAAGTCC TCCACCCCAA AGTCCTCCCA GCGGGTCCTT
    TTGGACCAAG AGTGTGTGTT TCAAGCCCAG AGCAAGTGGA AAGGTGCTGG AAAATTCATC
    CTCAGGCTAA AGGAGCAGGT GCAGGCATCC CGAGAAGATA AAAGAAAAGG CATTTCTTTC
    GCAAGTGAAT TCAAGAAGTT CACCAAATCA ACTAAACAGC CCCGAGGACT CACATCTTCT
    CAGGTCTTGG CCTCAGAAAG TGTCCAAAAC AAGGAGGAGA AGCCCACGGG CAGCTTGTCC
    TCCAGTGACA CCACAGACTC CCGACAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27697
    Clone ID Related Accession (Same CDS sequence) XM_022411588.1 , XM_005637460.2
    Accession Version XM_022411588.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6924bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006610.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGACTTCTG AAGGAATGGC AGCTTCTGTT CTCACACCTG TGACTCAAAG AAAAGTGACA 
    TTTGCTCCAT CGGCTGTAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAG TGTTCCAAAG
    GCACATAGTG TCAAGCAGAG TGAGCAGACT TCTACCATCC CGTGGATGAA CAAGCTTAAA
    GAAGAATCTT CTGGAAGCAA CTTGCCCAAA ATTCTCTCCA TAGCAAGGGA GAAAATAGCA
    AGTGATGAGA ACAGCAATGA AGAGTGCTGG ACGGAAAGCA CACCAGTTTC TGTGAAAAAC
    CTTAACATTA ACCATAACAA CATCCTGACA AACCGCCAGT GCGTCCTTCC TCAGAGCCAG
    TCTTATGACA CGTGCAATTC TGTCATGGAG GAAGACCCAT GTTTAGAAAC TGGAATCTCA
    TCCTCACTGG AAAGAAAGGT ATTCCCTGGA ATTCAACTGG AAGTCAACAG ACCTCCCATG
    GACTTTAGGC CTCCAGGCCT AATGGACTTT AGTACCTTAG GAAGTCAGTC AGCCATTGTA
    GATACTGGCC AGGCGCACCC TGACAGCAAC AAAGCAGCAT TTCAAATCTT TAATTATAAA
    GTTGACAGAA GGATGTCTGA CACTTTCTGT ACCCTGTCAG GTGACTTGAT ATTGGATGAC
    TGTGGAAACT GTGTGCCACT GTCATCTGGT TTTGGCGGGG AGCAAAAGAA AAATTACGTG
    GCATATACCT GTAAACTCAT GGAATTGGCT GAAAACTGTG ATAATGAGAA TGGACAGCTG
    CAGTGTGATG ATTATGATGC CTTGGATGAC AAATACTTGT GCTTTGAAGA TTCTTGCCAA
    AGGGACAGTG TGGTATGTTC AAGTGACAGC TTTCGCAGGG AGGACCTAAC TAACAGTCCA
    CCTGCCAAGA CCTTTCTGAG CCATTTTGAA GACTTCCCTG ATAATGGTGA AGATGTAGAA
    GATTTTTTAA AAAACAAAAA GGAGAGATCC ACTTTGTTAG TGAGGAGATT TTGTAAAAAT
    GACAGAGAAG TAAAGAAATC TGTGTATACT GGGACAAGGG CAATCATGAG AACTCTGCCT
    TCTGGTCACA TTGGGCTGGA AGCTTACAGT TACATCGATC AGAAGAGAAG TGGTCCCTTA
    TTACCCCGTG GGAGAGTTCT GGAACAGCTG CCAGTGGTGG CAATTAGGCA AGATGGGAGC
    CAGTGCCTGT CAGAAGCCCA GTGGTATCGG ATCTACAATG CAGTGAGAAG GGAAGAAGAA
    ATAGAAAATA CCATTGGATC TCTTCTCCAT TACTTTACCA AGCTCCCAGC CTCCAAGACA
    GCCCATGAAA GGATTAGTGT TGGTCCATGC CTAAAGCAGT GTGTCCGAGA CACCATATGT
    GAGTATCGTG CCACCCTCCA GAGGACGTCC ATATCTCAGT ACATCACGGG TTCGCTCCTA
    GAAGCAACCA CATCTTTAGG AGCGAGAAGT GGCCTACTCA GTACTTTTGG AGGATCCACT
    GGACGAATGA TGCTAAAAGA ACGCCAGCCA GGCACCTCCA TGGCCAATTC CAGTGCCCTC
    CCTTCAAGTT CCGCTGGAAT CAGCAAGGAG CTGATTGACC TGCAGCCTCT CATTCAGTTC
    CCAGAGGAAG TCGCCAGCAT CCTGATGGAG CAGGAACAGA ACATTTACCG AAGGGTCTTA
    CCAGTTGACT ATCTTTACTT CTTGACCCGG GACTTGGGCA CTCCAGAATG CCAGACACCC
    CTGCCCTGCC TCAAAGCATC CATCTCAGCT TCAATTCTTA GCTCCCAGAA CGGAGAGCAC
    AATGCCCTGG AAGATCTTGT GATGAGATTT AATGAGGTGA GCTCCTGGGT GACATGGCTG
    ATCCTCACGG CGGGCTCCAT GGAGGAGAAG CGAGAAGTCT TCTCCTATTT GGTGCATGTG
    GCCAAATGCT GCTGGAACAT GGGCAACTAC AATGCTGTCA TGGAGTTCTT GGCTGGTCTC
    AGGTCAAGAA AAGTTTTAAA AATGTGGCAG TTTATGGACC AATCTGACCT CGAGACCATG
    AGGAGCCTGA AGGACGCCAT GGCTCAGCAT GAATCTTCCT GCGAATATCG GAAGGTGATA
    ACGAGGGCTC TGCACATACC TGGCTGTAAA GTGGTTCCAT TCTGTGGAGT GTTTCTGAAG
    GAGCTCTGTG AAGTACTGGA CGGAGCCTCT GGCCTCATGA AGCTTTGCCC GAGGTACAAT
    TCCCAAGAAG AAACTCTAGA GTTTGTAGCA GATTACAGTG GACAAGATAA TTTCTTACAA
    CGAGTGGGGC ATAATGGCTT AAAGAATTCA GAGAAGGAGT CCACTGTCAA CAGCATCTTT
    CAAATCATTA GGAGCTGCAG CCGAAGTCTG GAGGCTGAGG AAGAGGACAG CCCCAGTGAA
    GGGAACAGCT CTAGGAAAAA TTCTTTAAGG GACAAAGCCC GATGGCAATT TATAATCGGA
    GATTTGTTGG ATTCCGAAAA TGACATCTTT GAGCAATCCA AAGAATGGGA CTCTCCCAGC
    TCAGAAGAGC CTCAGAAAGC CTTTGACCAT GGGACAGAGC TGATCCCTTG GTACGTGCTG
    TCCATCCGAG CAGATGTGCA CCAGTTCCTG CTACAGGGCG CCACCGTCCT CCGCTACGAC
    CAGGACACGC ACCTCTCTGC CCGCTGCTTC CTCCAGCTTC AGCCTGACAA TAGCACCTTG
    ACATGGATAA AGCCCACAGC TGCCTCCCCG GCCAGTGCTA GAGCAAAACT CGGTGTGCTG
    AGCAACACGT CTGAGCCTGG CAAGTTCCCG TCACCAGGCA GTGCCGGACT AAGCGGCCTG
    GCCGAGGGGG TCCTGGATCT GTTTTCAGCA AAGGCTGTGT ACATGGGACA CCCTAGCATT
    GATATCCACA CTGTGTGTGT CCAGAACAAA CTCGGTAGCA TGTTTCTCTC AGAGACTGGC
    GTGACCCTGC TCTACGGGCT CCAGACCACA GACAATAGAT TATTGCACTT TGTGGCACCC
    AAACACACTG CTAAGATGCT CTTCAGTGGA TTGCTAGAAC TCACGAGGGC CGTGAGGAAG
    ATGAGAAAGT TCCCTGACCA GAGGCAGCAG TGGCTGCGGA AACAGTATGT TAGCCTCTAT
    CAGGAGGACG GACGGTATGA GGGCCCAACT TTGGCTCATG CTGTGGAGTT GTTTGGTGGC
    CGGCGATGGA GTACACGCAA TCCCAGCCCT GGGACATCTG CAAAGAGTGC TGAGAAGCCC
    AGTGTGCAGA GAAACAACAC CTTGGGCATA AGCACCACCA AGAAAAAGAA GAAAATCCTT
    ATAAGGGGTG AGAGTGGAGA GGCAGCCGAC GATGAGATGG CCACCCGGAA GGCCAAAATG
    CACAGGGAGT GTCGCAGCCG GAGTGGTTCT GACCCACAAG ACATGAATGA GCAAGAAGAA
    TCTGAGGCAA ATGCCATCAT GAGCCCTCCT AACACTCTGC CTTCCAGAAG AGCCCACTCT
    TTGACTACAG CTGGGTCCCC TAATTTGTCT GCCGGGACAT CATCTCCCAT CAGTGCTTGG
    AGCAGCAGCA GCTGGCATGG AAGGATCAAA GGAGGAATGA AGGGTTTTCA GAGCTTCATG
    GTTTCAGACA GTAACATGAG TTTTGTTGAA TTTGTTGAGC TGTTCAAATC ATTCAGTGTG
    AGGAGCCGCA AGGACCTGAA GGATCTCTTT GACATCTACG CTGTGCCCTG CAATCGGGCC
    GGCTCTGAGT CGGCCCCGCT CTACACCAAC CTAACAATAG ACGAAAACAC CAGCGATCTT
    CAGCCTGACC TAGATTTATT GACCAGAAAC GTCTCGGATT TGGGGTTGTT CATTAAGAGT
    AAACAGCAGC TGTCAGACAA CCAGAGGCAG ATTTCTGATG CCATTGCTGC TGCAAGTATC
    GTGACGAATG GCACTGGGAT CGAGAGCACA TCTTTGGGCA TCTTTGGGGT TGGCATACTC
    CAGCTCAATG ACTTCCTAGT CAACTGCCAG GGAGAACACT GCACTTACGA TGAAATCCTC
    AGCATCATCC AGAAGTTCGA GCCTAGCATT AGTATGTGTC ATCAGGGCCT ACTGTCCTTT
    GAAGGATTTG CAAGGTTTCT GATGGATAAA GATAATTTTG CCTCCAAAAA TGATGAGTCA
    CAGGAGAACA TTAAAGATTT GCAGCTGCCT CTCTCCTACT ATTACATTGA GTCTTCACAC
    AATACCTACC TCACAGGCCA CCAGCTCAAA GGAGAGTCAT CGGTAGAACT CTATAGCCAG
    GTCCTCCTGC AAGGCTGTAG AAGTGTAGAG TTGGACTGCT GGGATGGAGA TGACGGGATG
    CCCATCATCT ATCACGGACA CACACTAACA ACCAAGATCC CCTTCAAGGA GGTGGTTGAA
    GCCATCGACC GCAGTGCCTT CATCAACTCT GACCTGCCCA TCATCATATC GATTGAGAAC
    CACTGCTCGT TGCCTCAGCA ACGAAAAATG GCAGAAATTT TCAAGACTGT GTTTGGAGAA
    AAACTGGTGG CTAAATTCCT GTTTGAGAGC GATTTCTCAG ATGATCCAAT GCTTCCCTCA
    CCTGACCAAC TTAGGAGGAA AGTGCTTCTT AAAAATAAGA AGCTAAAAGC CCACCAGACA
    CCAGTGGATA TCTTAAAGCA AAAGGCTCAT CAGTTAGCAT CCATGCAAGC TCAGGCTTAT
    AATGGGGGAA ATGTCAACCC TCCACCTGCT AATAACGAGG AAGAGGAAGA TGAAGAGGAC
    GAATATGATT ATGACTATGA ATCCCTGTCT GATGCAGATG TCCTTACTGC TTCTCCTGCT
    CCTAACAGTC AGGAAGACAA CATTCTAGAA GACAGACCTG AAAATAAATC ATGTAATGAC
    AAGCTTCAGT TTGAGTATAA CGAAGAAATC CCCAAGAGGA TAAAGAAAGC AGATAATTCT
    GCTTTCAACA AAGGAAAGGT TTATGACATG GAACTGGGAG AAGAATTTTA TCTTCCTCAG
    AATAAAAAGG AAAGCAGACA GATTGCACCA GAGCTTTCTG ACCTTGTCAT CTACTGCCAA
    GCGGTAAAGT TTCCGGGGCT GTCAACTCTA AATGCATCTG GCTCTAACAG AGGAAAAGAG
    AGGAAAAGCA GGAAGTCCAT TTTTGGCAAC AATCCGGGCA GAATGAGCCC TGGGGAGACA
    GCATCGTTTA ACAAAGCATC TGGAAAAAGT TCCTGTGAAG GAATGCGACA GGCGTGGGAG
    GAATCTTCTT CCCCCCTCAA CCCAACCACA TCCCTCAGCG CTATCATTAG AACTCCCAAA
    TGTTACCATA TCTCATCGCT GAATGAAAAT GCCGCCAAAC GTCTGTGTCG CAGGTATTCT
    CAGAAACTGA TCCAGCACAC TGCCTGTCAG CTGCTGAGGA CTTACCCGGC TGCCACACGC
    ATCGACTCCT CCAACCCCAA TCCTCTCTTG TTCTGGCTCC ATGGGATACA GCTCGTGGCA
    CTCAACTACC AGACAGATGA TCTCCCTTTA CATTTAAATG CTGCCATGTT TGAGGCCAAC
    GGTGGCTGTG GTTATGTTTT GAAACCCCCC GTTCTGTGGG ACAAGAACTG TCCCATGTAC
    CAGAAGTTTT CACCCTTGGA AAGAGACCTG GACAACATGG AGCCTGCCGT CTATTCCTTA
    ACTATTGTCT CTGGTCAAAA CGTGTGCCCA AGTAGCAGCA CGGGAAGCCC ATGTATCGAA
    GTGGACGTGC TGGGCATGCC CCTGGACAGT TGCCACTTCC GCACAAAACC CATCCACCGG
    AACACTTTGA ACCCCATGTG GAATGAACAG TTTCTCTTCC GGGTTCACTT TGAAGATCTC
    ATATTCCTGC GTTTTGCAGT TGTGGAAAAC AATAGCTCAG CCATAACCGC TCAGAGAATC
    ATCCCCCTCA AGGCTTTAAA ACGAGGATAT CGACATCTTC AGCTGCGAAA CCTCCACAAT
    GAAGTCTTGG AAATCTCTAG TTTATTCATA AACAGCAGAA GGATGGAAGA AAATTCCTCC
    AGCAGTGCCA CGCCAGCCTC TTTGATGTTT AATACAGAAG AAAGAAAATG TTTGCAGACT
    CACAGAGTCA CAGTGCATGG GGTCCCAGGT CCAGAGCCCT TTACTGTTTT CTCCATAAGT
    GGAGGCACGA AGGCAAAGCA GCTTCTCCAA CAGATTCTAA CTACTGAACA AGACACCAAA
    CCTATTGTCA CAGACTATTT TTTGATGGAA GAAAAATATT TTATATCTAA AGAAAAGAAT
    GAATGCAGGA AACAACCATT TCAGAGAGTC ATCGGTCCAG AAGAAGAGAT CATGCAGATT
    TTAAGCAGCT GGTTTCCAGA GGAAGGATAT GTTGGCAGGA TTGTCTTAAA AACCCAGCAG
    GAAAACCTAG AAGAGAGAAG CATTGTTCAA GATGAGAAAG AGGTGATCTT GAGCTCGGAG
    GAGGAGAGCT TCTTTGTCCA AGTGCATGAC GTTTCTCCAG AGCAACCTCG GACGGTCATC
    AAAGCGCCCC GTGTCAGCAC CGCTCAGGAT GTCATTCAAC AGACCTTATG CAAAGCCAAA
    TATTCCTATA GCATCCTGAG TAACCCCAAC CCGAGTGACT ATGTGCTTCT GGAAGAGGTG
    GTGAAAGATG CTACCAAGAA GTCCTCCACC CCAAAGTCCT CCCAGCGGGT CCTTTTGGAC
    CAAGAGTGTG TGTTTCAAGC CCAGAGCAAG TGGAAAGGTG CTGGAAAATT CATCCTCAGG
    CTAAAGGAGC AGGTGCAGGC ATCCCGAGAA GATAAAAGAA AAGGCATTTC TTTCGCAAGT
    GAATTCAAGA AGTTCACCAA ATCAACTAAA CAGCCCCGAG GACTCACATC TTCTCAGGTC
    TTGGCCTCAG AAAGTGTCCA AAACAAGGAG GAGAAGCCCA CGGGCAGCTT GTCCTCCAGT
    GACACCACAG ACTCCCGACA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022267296.1
    CDS352..7275
    Translation

    Target ORF information:

    RefSeq Version XM_022411588.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris phospholipase C epsilon 1 (PLCE1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022411588.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGACTTCTG AAGGAATGGC AGCTTCTGTT CTCACACCTG TGACTCAAAG AAAAGTGACA 
    TTTGCTCCAT CGGCTGTAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAG TGTTCCAAAG
    GCACATAGTG TCAAGCAGAG TGAGCAGACT TCTACCATCC CGTGGATGAA CAAGCTTAAA
    GAAGAATCTT CTGGAAGCAA CTTGCCCAAA ATTCTCTCCA TAGCAAGGGA GAAAATAGCA
    AGTGATGAGA ACAGCAATGA AGAGTGCTGG ACGGAAAGCA CACCAGTTTC TGTGAAAAAC
    CTTAACATTA ACCATAACAA CATCCTGACA AACCGCCAGT GCGTCCTTCC TCAGAGCCAG
    TCTTATGACA CGTGCAATTC TGTCATGGAG GAAGACCCAT GTTTAGAAAC TGGAATCTCA
    TCCTCACTGG AAAGAAAGGT ATTCCCTGGA ATTCAACTGG AAGTCAACAG ACCTCCCATG
    GACTTTAGGC CTCCAGGCCT AATGGACTTT AGTACCTTAG GAAGTCAGTC AGCCATTGTA
    GATACTGGCC AGGCGCACCC TGACAGCAAC AAAGCAGCAT TTCAAATCTT TAATTATAAA
    GTTGACAGAA GGATGTCTGA CACTTTCTGT ACCCTGTCAG GTGACTTGAT ATTGGATGAC
    TGTGGAAACT GTGTGCCACT GTCATCTGGT TTTGGCGGGG AGCAAAAGAA AAATTACGTG
    GCATATACCT GTAAACTCAT GGAATTGGCT GAAAACTGTG ATAATGAGAA TGGACAGCTG
    CAGTGTGATG ATTATGATGC CTTGGATGAC AAATACTTGT GCTTTGAAGA TTCTTGCCAA
    AGGGACAGTG TGGTATGTTC AAGTGACAGC TTTCGCAGGG AGGACCTAAC TAACAGTCCA
    CCTGCCAAGA CCTTTCTGAG CCATTTTGAA GACTTCCCTG ATAATGGTGA AGATGTAGAA
    GATTTTTTAA AAAACAAAAA GGAGAGATCC ACTTTGTTAG TGAGGAGATT TTGTAAAAAT
    GACAGAGAAG TAAAGAAATC TGTGTATACT GGGACAAGGG CAATCATGAG AACTCTGCCT
    TCTGGTCACA TTGGGCTGGA AGCTTACAGT TACATCGATC AGAAGAGAAG TGGTCCCTTA
    TTACCCCGTG GGAGAGTTCT GGAACAGCTG CCAGTGGTGG CAATTAGGCA AGATGGGAGC
    CAGTGCCTGT CAGAAGCCCA GTGGTATCGG ATCTACAATG CAGTGAGAAG GGAAGAAGAA
    ATAGAAAATA CCATTGGATC TCTTCTCCAT TACTTTACCA AGCTCCCAGC CTCCAAGACA
    GCCCATGAAA GGATTAGTGT TGGTCCATGC CTAAAGCAGT GTGTCCGAGA CACCATATGT
    GAGTATCGTG CCACCCTCCA GAGGACGTCC ATATCTCAGT ACATCACGGG TTCGCTCCTA
    GAAGCAACCA CATCTTTAGG AGCGAGAAGT GGCCTACTCA GTACTTTTGG AGGATCCACT
    GGACGAATGA TGCTAAAAGA ACGCCAGCCA GGCACCTCCA TGGCCAATTC CAGTGCCCTC
    CCTTCAAGTT CCGCTGGAAT CAGCAAGGAG CTGATTGACC TGCAGCCTCT CATTCAGTTC
    CCAGAGGAAG TCGCCAGCAT CCTGATGGAG CAGGAACAGA ACATTTACCG AAGGGTCTTA
    CCAGTTGACT ATCTTTACTT CTTGACCCGG GACTTGGGCA CTCCAGAATG CCAGACACCC
    CTGCCCTGCC TCAAAGCATC CATCTCAGCT TCAATTCTTA GCTCCCAGAA CGGAGAGCAC
    AATGCCCTGG AAGATCTTGT GATGAGATTT AATGAGGTGA GCTCCTGGGT GACATGGCTG
    ATCCTCACGG CGGGCTCCAT GGAGGAGAAG CGAGAAGTCT TCTCCTATTT GGTGCATGTG
    GCCAAATGCT GCTGGAACAT GGGCAACTAC AATGCTGTCA TGGAGTTCTT GGCTGGTCTC
    AGGTCAAGAA AAGTTTTAAA AATGTGGCAG TTTATGGACC AATCTGACCT CGAGACCATG
    AGGAGCCTGA AGGACGCCAT GGCTCAGCAT GAATCTTCCT GCGAATATCG GAAGGTGATA
    ACGAGGGCTC TGCACATACC TGGCTGTAAA GTGGTTCCAT TCTGTGGAGT GTTTCTGAAG
    GAGCTCTGTG AAGTACTGGA CGGAGCCTCT GGCCTCATGA AGCTTTGCCC GAGGTACAAT
    TCCCAAGAAG AAACTCTAGA GTTTGTAGCA GATTACAGTG GACAAGATAA TTTCTTACAA
    CGAGTGGGGC ATAATGGCTT AAAGAATTCA GAGAAGGAGT CCACTGTCAA CAGCATCTTT
    CAAATCATTA GGAGCTGCAG CCGAAGTCTG GAGGCTGAGG AAGAGGACAG CCCCAGTGAA
    GGGAACAGCT CTAGGAAAAA TTCTTTAAGG GACAAAGCCC GATGGCAATT TATAATCGGA
    GATTTGTTGG ATTCCGAAAA TGACATCTTT GAGCAATCCA AAGAATGGGA CTCTCCCAGC
    TCAGAAGAGC CTCAGAAAGC CTTTGACCAT GGGACAGAGC TGATCCCTTG GTACGTGCTG
    TCCATCCGAG CAGATGTGCA CCAGTTCCTG CTACAGGGCG CCACCGTCCT CCGCTACGAC
    CAGGACACGC ACCTCTCTGC CCGCTGCTTC CTCCAGCTTC AGCCTGACAA TAGCACCTTG
    ACATGGATAA AGCCCACAGC TGCCTCCCCG GCCAGTGCTA GAGCAAAACT CGGTGTGCTG
    AGCAACACGT CTGAGCCTGG CAAGTTCCCG TCACCAGGCA GTGCCGGACT AAGCGGCCTG
    GCCGAGGGGG TCCTGGATCT GTTTTCAGCA AAGGCTGTGT ACATGGGACA CCCTAGCATT
    GATATCCACA CTGTGTGTGT CCAGAACAAA CTCGGTAGCA TGTTTCTCTC AGAGACTGGC
    GTGACCCTGC TCTACGGGCT CCAGACCACA GACAATAGAT TATTGCACTT TGTGGCACCC
    AAACACACTG CTAAGATGCT CTTCAGTGGA TTGCTAGAAC TCACGAGGGC CGTGAGGAAG
    ATGAGAAAGT TCCCTGACCA GAGGCAGCAG TGGCTGCGGA AACAGTATGT TAGCCTCTAT
    CAGGAGGACG GACGGTATGA GGGCCCAACT TTGGCTCATG CTGTGGAGTT GTTTGGTGGC
    CGGCGATGGA GTACACGCAA TCCCAGCCCT GGGACATCTG CAAAGAGTGC TGAGAAGCCC
    AGTGTGCAGA GAAACAACAC CTTGGGCATA AGCACCACCA AGAAAAAGAA GAAAATCCTT
    ATAAGGGGTG AGAGTGGAGA GGCAGCCGAC GATGAGATGG CCACCCGGAA GGCCAAAATG
    CACAGGGAGT GTCGCAGCCG GAGTGGTTCT GACCCACAAG ACATGAATGA GCAAGAAGAA
    TCTGAGGCAA ATGCCATCAT GAGCCCTCCT AACACTCTGC CTTCCAGAAG AGCCCACTCT
    TTGACTACAG CTGGGTCCCC TAATTTGTCT GCCGGGACAT CATCTCCCAT CAGTGCTTGG
    AGCAGCAGCA GCTGGCATGG AAGGATCAAA GGAGGAATGA AGGGTTTTCA GAGCTTCATG
    GTTTCAGACA GTAACATGAG TTTTGTTGAA TTTGTTGAGC TGTTCAAATC ATTCAGTGTG
    AGGAGCCGCA AGGACCTGAA GGATCTCTTT GACATCTACG CTGTGCCCTG CAATCGGGCC
    GGCTCTGAGT CGGCCCCGCT CTACACCAAC CTAACAATAG ACGAAAACAC CAGCGATCTT
    CAGCCTGACC TAGATTTATT GACCAGAAAC GTCTCGGATT TGGGGTTGTT CATTAAGAGT
    AAACAGCAGC TGTCAGACAA CCAGAGGCAG ATTTCTGATG CCATTGCTGC TGCAAGTATC
    GTGACGAATG GCACTGGGAT CGAGAGCACA TCTTTGGGCA TCTTTGGGGT TGGCATACTC
    CAGCTCAATG ACTTCCTAGT CAACTGCCAG GGAGAACACT GCACTTACGA TGAAATCCTC
    AGCATCATCC AGAAGTTCGA GCCTAGCATT AGTATGTGTC ATCAGGGCCT ACTGTCCTTT
    GAAGGATTTG CAAGGTTTCT GATGGATAAA GATAATTTTG CCTCCAAAAA TGATGAGTCA
    CAGGAGAACA TTAAAGATTT GCAGCTGCCT CTCTCCTACT ATTACATTGA GTCTTCACAC
    AATACCTACC TCACAGGCCA CCAGCTCAAA GGAGAGTCAT CGGTAGAACT CTATAGCCAG
    GTCCTCCTGC AAGGCTGTAG AAGTGTAGAG TTGGACTGCT GGGATGGAGA TGACGGGATG
    CCCATCATCT ATCACGGACA CACACTAACA ACCAAGATCC CCTTCAAGGA GGTGGTTGAA
    GCCATCGACC GCAGTGCCTT CATCAACTCT GACCTGCCCA TCATCATATC GATTGAGAAC
    CACTGCTCGT TGCCTCAGCA ACGAAAAATG GCAGAAATTT TCAAGACTGT GTTTGGAGAA
    AAACTGGTGG CTAAATTCCT GTTTGAGAGC GATTTCTCAG ATGATCCAAT GCTTCCCTCA
    CCTGACCAAC TTAGGAGGAA AGTGCTTCTT AAAAATAAGA AGCTAAAAGC CCACCAGACA
    CCAGTGGATA TCTTAAAGCA AAAGGCTCAT CAGTTAGCAT CCATGCAAGC TCAGGCTTAT
    AATGGGGGAA ATGTCAACCC TCCACCTGCT AATAACGAGG AAGAGGAAGA TGAAGAGGAC
    GAATATGATT ATGACTATGA ATCCCTGTCT GATGCAGATG TCCTTACTGC TTCTCCTGCT
    CCTAACAGTC AGGAAGACAA CATTCTAGAA GACAGACCTG AAAATAAATC ATGTAATGAC
    AAGCTTCAGT TTGAGTATAA CGAAGAAATC CCCAAGAGGA TAAAGAAAGC AGATAATTCT
    GCTTTCAACA AAGGAAAGGT TTATGACATG GAACTGGGAG AAGAATTTTA TCTTCCTCAG
    AATAAAAAGG AAAGCAGACA GATTGCACCA GAGCTTTCTG ACCTTGTCAT CTACTGCCAA
    GCGGTAAAGT TTCCGGGGCT GTCAACTCTA AATGCATCTG GCTCTAACAG AGGAAAAGAG
    AGGAAAAGCA GGAAGTCCAT TTTTGGCAAC AATCCGGGCA GAATGAGCCC TGGGGAGACA
    GCATCGTTTA ACAAAGCATC TGGAAAAAGT TCCTGTGAAG GAATGCGACA GGCGTGGGAG
    GAATCTTCTT CCCCCCTCAA CCCAACCACA TCCCTCAGCG CTATCATTAG AACTCCCAAA
    TGTTACCATA TCTCATCGCT GAATGAAAAT GCCGCCAAAC GTCTGTGTCG CAGGTATTCT
    CAGAAACTGA TCCAGCACAC TGCCTGTCAG CTGCTGAGGA CTTACCCGGC TGCCACACGC
    ATCGACTCCT CCAACCCCAA TCCTCTCTTG TTCTGGCTCC ATGGGATACA GCTCGTGGCA
    CTCAACTACC AGACAGATGA TCTCCCTTTA CATTTAAATG CTGCCATGTT TGAGGCCAAC
    GGTGGCTGTG GTTATGTTTT GAAACCCCCC GTTCTGTGGG ACAAGAACTG TCCCATGTAC
    CAGAAGTTTT CACCCTTGGA AAGAGACCTG GACAACATGG AGCCTGCCGT CTATTCCTTA
    ACTATTGTCT CTGGTCAAAA CGTGTGCCCA AGTAGCAGCA CGGGAAGCCC ATGTATCGAA
    GTGGACGTGC TGGGCATGCC CCTGGACAGT TGCCACTTCC GCACAAAACC CATCCACCGG
    AACACTTTGA ACCCCATGTG GAATGAACAG TTTCTCTTCC GGGTTCACTT TGAAGATCTC
    ATATTCCTGC GTTTTGCAGT TGTGGAAAAC AATAGCTCAG CCATAACCGC TCAGAGAATC
    ATCCCCCTCA AGGCTTTAAA ACGAGGATAT CGACATCTTC AGCTGCGAAA CCTCCACAAT
    GAAGTCTTGG AAATCTCTAG TTTATTCATA AACAGCAGAA GGATGGAAGA AAATTCCTCC
    AGCAGTGCCA CGCCAGCCTC TTTGATGTTT AATACAGAAG AAAGAAAATG TTTGCAGACT
    CACAGAGTCA CAGTGCATGG GGTCCCAGGT CCAGAGCCCT TTACTGTTTT CTCCATAAGT
    GGAGGCACGA AGGCAAAGCA GCTTCTCCAA CAGATTCTAA CTACTGAACA AGACACCAAA
    CCTATTGTCA CAGACTATTT TTTGATGGAA GAAAAATATT TTATATCTAA AGAAAAGAAT
    GAATGCAGGA AACAACCATT TCAGAGAGTC ATCGGTCCAG AAGAAGAGAT CATGCAGATT
    TTAAGCAGCT GGTTTCCAGA GGAAGGATAT GTTGGCAGGA TTGTCTTAAA AACCCAGCAG
    GAAAACCTAG AAGAGAGAAG CATTGTTCAA GATGAGAAAG AGGTGATCTT GAGCTCGGAG
    GAGGAGAGCT TCTTTGTCCA AGTGCATGAC GTTTCTCCAG AGCAACCTCG GACGGTCATC
    AAAGCGCCCC GTGTCAGCAC CGCTCAGGAT GTCATTCAAC AGACCTTATG CAAAGCCAAA
    TATTCCTATA GCATCCTGAG TAACCCCAAC CCGAGTGACT ATGTGCTTCT GGAAGAGGTG
    GTGAAAGATG CTACCAAGAA GTCCTCCACC CCAAAGTCCT CCCAGCGGGT CCTTTTGGAC
    CAAGAGTGTG TGTTTCAAGC CCAGAGCAAG TGGAAAGGTG CTGGAAAATT CATCCTCAGG
    CTAAAGGAGC AGGTGCAGGC ATCCCGAGAA GATAAAAGAA AAGGCATTTC TTTCGCAAGT
    GAATTCAAGA AGTTCACCAA ATCAACTAAA CAGCCCCGAG GACTCACATC TTCTCAGGTC
    TTGGCCTCAG AAAGTGTCCA AAACAAGGAG GAGAAGCCCA CGGGCAGCTT GTCCTCCAGT
    GACACCACAG ACTCCCGACA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27697
    Clone ID Related Accession (Same CDS sequence) XM_022411588.1 , XM_005637460.2
    Accession Version XM_005637460.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6924bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005637460.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGACTTCTG AAGGAATGGC AGCTTCTGTT CTCACACCTG TGACTCAAAG AAAAGTGACA 
    TTTGCTCCAT CGGCTGTAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAG TGTTCCAAAG
    GCACATAGTG TCAAGCAGAG TGAGCAGACT TCTACCATCC CGTGGATGAA CAAGCTTAAA
    GAAGAATCTT CTGGAAGCAA CTTGCCCAAA ATTCTCTCCA TAGCAAGGGA GAAAATAGCA
    AGTGATGAGA ACAGCAATGA AGAGTGCTGG ACGGAAAGCA CACCAGTTTC TGTGAAAAAC
    CTTAACATTA ACCATAACAA CATCCTGACA AACCGCCAGT GCGTCCTTCC TCAGAGCCAG
    TCTTATGACA CGTGCAATTC TGTCATGGAG GAAGACCCAT GTTTAGAAAC TGGAATCTCA
    TCCTCACTGG AAAGAAAGGT ATTCCCTGGA ATTCAACTGG AAGTCAACAG ACCTCCCATG
    GACTTTAGGC CTCCAGGCCT AATGGACTTT AGTACCTTAG GAAGTCAGTC AGCCATTGTA
    GATACTGGCC AGGCGCACCC TGACAGCAAC AAAGCAGCAT TTCAAATCTT TAATTATAAA
    GTTGACAGAA GGATGTCTGA CACTTTCTGT ACCCTGTCAG GTGACTTGAT ATTGGATGAC
    TGTGGAAACT GTGTGCCACT GTCATCTGGT TTTGGCGGGG AGCAAAAGAA AAATTACGTG
    GCATATACCT GTAAACTCAT GGAATTGGCT GAAAACTGTG ATAATGAGAA TGGACAGCTG
    CAGTGTGATG ATTATGATGC CTTGGATGAC AAATACTTGT GCTTTGAAGA TTCTTGCCAA
    AGGGACAGTG TGGTATGTTC AAGTGACAGC TTTCGCAGGG AGGACCTAAC TAACAGTCCA
    CCTGCCAAGA CCTTTCTGAG CCATTTTGAA GACTTCCCTG ATAATGGTGA AGATGTAGAA
    GATTTTTTAA AAAACAAAAA GGAGAGATCC ACTTTGTTAG TGAGGAGATT TTGTAAAAAT
    GACAGAGAAG TAAAGAAATC TGTGTATACT GGGACAAGGG CAATCATGAG AACTCTGCCT
    TCTGGTCACA TTGGGCTGGA AGCTTACAGT TACATCGATC AGAAGAGAAG TGGTCCCTTA
    TTACCCCGTG GGAGAGTTCT GGAACAGCTG CCAGTGGTGG CAATTAGGCA AGATGGGAGC
    CAGTGCCTGT CAGAAGCCCA GTGGTATCGG ATCTACAATG CAGTGAGAAG GGAAGAAGAA
    ATAGAAAATA CCATTGGATC TCTTCTCCAT TACTTTACCA AGCTCCCAGC CTCCAAGACA
    GCCCATGAAA GGATTAGTGT TGGTCCATGC CTAAAGCAGT GTGTCCGAGA CACCATATGT
    GAGTATCGTG CCACCCTCCA GAGGACGTCC ATATCTCAGT ACATCACGGG TTCGCTCCTA
    GAAGCAACCA CATCTTTAGG AGCGAGAAGT GGCCTACTCA GTACTTTTGG AGGATCCACT
    GGACGAATGA TGCTAAAAGA ACGCCAGCCA GGCACCTCCA TGGCCAATTC CAGTGCCCTC
    CCTTCAAGTT CCGCTGGAAT CAGCAAGGAG CTGATTGACC TGCAGCCTCT CATTCAGTTC
    CCAGAGGAAG TCGCCAGCAT CCTGATGGAG CAGGAACAGA ACATTTACCG AAGGGTCTTA
    CCAGTTGACT ATCTTTACTT CTTGACCCGG GACTTGGGCA CTCCAGAATG CCAGACACCC
    CTGCCCTGCC TCAAAGCATC CATCTCAGCT TCAATTCTTA GCTCCCAGAA CGGAGAGCAC
    AATGCCCTGG AAGATCTTGT GATGAGATTT AATGAGGTGA GCTCCTGGGT GACATGGCTG
    ATCCTCACGG CGGGCTCCAT GGAGGAGAAG CGAGAAGTCT TCTCCTATTT GGTGCATGTG
    GCCAAATGCT GCTGGAACAT GGGCAACTAC AATGCTGTCA TGGAGTTCTT GGCTGGTCTC
    AGGTCAAGAA AAGTTTTAAA AATGTGGCAG TTTATGGACC AATCTGACCT CGAGACCATG
    AGGAGCCTGA AGGACGCCAT GGCTCAGCAT GAATCTTCCT GCGAATATCG GAAGGTGATA
    ACGAGGGCTC TGCACATACC TGGCTGTAAA GTGGTTCCAT TCTGTGGAGT GTTTCTGAAG
    GAGCTCTGTG AAGTACTGGA CGGAGCCTCT GGCCTCATGA AGCTTTGCCC GAGGTACAAT
    TCCCAAGAAG AAACTCTAGA GTTTGTAGCA GATTACAGTG GACAAGATAA TTTCTTACAA
    CGAGTGGGGC ATAATGGCTT AAAGAATTCA GAGAAGGAGT CCACTGTCAA CAGCATCTTT
    CAAATCATTA GGAGCTGCAG CCGAAGTCTG GAGGCTGAGG AAGAGGACAG CCCCAGTGAA
    GGGAACAGCT CTAGGAAAAA TTCTTTAAGG GACAAAGCCC GATGGCAATT TATAATCGGA
    GATTTGTTGG ATTCCGAAAA TGACATCTTT GAGCAATCCA AAGAATGGGA CTCTCCCAGC
    TCAGAAGAGC CTCAGAAAGC CTTTGACCAT GGGACAGAGC TGATCCCTTG GTACGTGCTG
    TCCATCCGAG CAGATGTGCA CCAGTTCCTG CTACAGGGCG CCACCGTCCT CCGCTACGAC
    CAGGACACGC ACCTCTCTGC CCGCTGCTTC CTCCAGCTTC AGCCTGACAA TAGCACCTTG
    ACATGGATAA AGCCCACAGC TGCCTCCCCG GCCAGTGCTA GAGCAAAACT CGGTGTGCTG
    AGCAACACGT CTGAGCCTGG CAAGTTCCCG TCACCAGGCA GTGCCGGACT AAGCGGCCTG
    GCCGAGGGGG TCCTGGATCT GTTTTCAGCA AAGGCTGTGT ACATGGGACA CCCTAGCATT
    GATATCCACA CTGTGTGTGT CCAGAACAAA CTCGGTAGCA TGTTTCTCTC AGAGACTGGC
    GTGACCCTGC TCTACGGGCT CCAGACCACA GACAATAGAT TATTGCACTT TGTGGCACCC
    AAACACACTG CTAAGATGCT CTTCAGTGGA TTGCTAGAAC TCACGAGGGC CGTGAGGAAG
    ATGAGAAAGT TCCCTGACCA GAGGCAGCAG TGGCTGCGGA AACAGTATGT TAGCCTCTAT
    CAGGAGGACG GACGGTATGA GGGCCCAACT TTGGCTCATG CTGTGGAGTT GTTTGGTGGC
    CGGCGATGGA GTACACGCAA TCCCAGCCCT GGGACATCTG CAAAGAGTGC TGAGAAGCCC
    AGTGTGCAGA GAAACAACAC CTTGGGCATA AGCACCACCA AGAAAAAGAA GAAAATCCTT
    ATAAGGGGTG AGAGTGGAGA GGCAGCCGAC GATGAGATGG CCACCCGGAA GGCCAAAATG
    CACAGGGAGT GTCGCAGCCG GAGTGGTTCT GACCCACAAG ACATGAATGA GCAAGAAGAA
    TCTGAGGCAA ATGCCATCAT GAGCCCTCCT AACACTCTGC CTTCCAGAAG AGCCCACTCT
    TTGACTACAG CTGGGTCCCC TAATTTGTCT GCCGGGACAT CATCTCCCAT CAGTGCTTGG
    AGCAGCAGCA GCTGGCATGG AAGGATCAAA GGAGGAATGA AGGGTTTTCA GAGCTTCATG
    GTTTCAGACA GTAACATGAG TTTTGTTGAA TTTGTTGAGC TGTTCAAATC ATTCAGTGTG
    AGGAGCCGCA AGGACCTGAA GGATCTCTTT GACATCTACG CTGTGCCCTG CAATCGGGCC
    GGCTCTGAGT CGGCCCCGCT CTACACCAAC CTAACAATAG ACGAAAACAC CAGCGATCTT
    CAGCCTGACC TAGATTTATT GACCAGAAAC GTCTCGGATT TGGGGTTGTT CATTAAGAGT
    AAACAGCAGC TGTCAGACAA CCAGAGGCAG ATTTCTGATG CCATTGCTGC TGCAAGTATC
    GTGACGAATG GCACTGGGAT CGAGAGCACA TCTTTGGGCA TCTTTGGGGT TGGCATACTC
    CAGCTCAATG ACTTCCTAGT CAACTGCCAG GGAGAACACT GCACTTACGA TGAAATCCTC
    AGCATCATCC AGAAGTTCGA GCCTAGCATT AGTATGTGTC ATCAGGGCCT ACTGTCCTTT
    GAAGGATTTG CAAGGTTTCT GATGGATAAA GATAATTTTG CCTCCAAAAA TGATGAGTCA
    CAGGAGAACA TTAAAGATTT GCAGCTGCCT CTCTCCTACT ATTACATTGA GTCTTCACAC
    AATACCTACC TCACAGGCCA CCAGCTCAAA GGAGAGTCAT CGGTAGAACT CTATAGCCAG
    GTCCTCCTGC AAGGCTGTAG AAGTGTAGAG TTGGACTGCT GGGATGGAGA TGACGGGATG
    CCCATCATCT ATCACGGACA CACACTAACA ACCAAGATCC CCTTCAAGGA GGTGGTTGAA
    GCCATCGACC GCAGTGCCTT CATCAACTCT GACCTGCCCA TCATCATATC GATTGAGAAC
    CACTGCTCGT TGCCTCAGCA ACGAAAAATG GCAGAAATTT TCAAGACTGT GTTTGGAGAA
    AAACTGGTGG CTAAATTCCT GTTTGAGAGC GATTTCTCAG ATGATCCAAT GCTTCCCTCA
    CCTGACCAAC TTAGGAGGAA AGTGCTTCTT AAAAATAAGA AGCTAAAAGC CCACCAGACA
    CCAGTGGATA TCTTAAAGCA AAAGGCTCAT CAGTTAGCAT CCATGCAAGC TCAGGCTTAT
    AATGGGGGAA ATGTCAACCC TCCACCTGCT AATAACGAGG AAGAGGAAGA TGAAGAGGAC
    GAATATGATT ATGACTATGA ATCCCTGTCT GATGCAGATG TCCTTACTGC TTCTCCTGCT
    CCTAACAGTC AGGAAGACAA CATTCTAGAA GACAGACCTG AAAATAAATC ATGTAATGAC
    AAGCTTCAGT TTGAGTATAA CGAAGAAATC CCCAAGAGGA TAAAGAAAGC AGATAATTCT
    GCTTTCAACA AAGGAAAGGT TTATGACATG GAACTGGGAG AAGAATTTTA TCTTCCTCAG
    AATAAAAAGG AAAGCAGACA GATTGCACCA GAGCTTTCTG ACCTTGTCAT CTACTGCCAA
    GCGGTAAAGT TTCCGGGGCT GTCAACTCTA AATGCATCTG GCTCTAACAG AGGAAAAGAG
    AGGAAAAGCA GGAAGTCCAT TTTTGGCAAC AATCCGGGCA GAATGAGCCC TGGGGAGACA
    GCATCGTTTA ACAAAGCATC TGGAAAAAGT TCCTGTGAAG GAATGCGACA GGCGTGGGAG
    GAATCTTCTT CCCCCCTCAA CCCAACCACA TCCCTCAGCG CTATCATTAG AACTCCCAAA
    TGTTACCATA TCTCATCGCT GAATGAAAAT GCCGCCAAAC GTCTGTGTCG CAGGTATTCT
    CAGAAACTGA TCCAGCACAC TGCCTGTCAG CTGCTGAGGA CTTACCCGGC TGCCACACGC
    ATCGACTCCT CCAACCCCAA TCCTCTCTTG TTCTGGCTCC ATGGGATACA GCTCGTGGCA
    CTCAACTACC AGACAGATGA TCTCCCTTTA CATTTAAATG CTGCCATGTT TGAGGCCAAC
    GGTGGCTGTG GTTATGTTTT GAAACCCCCC GTTCTGTGGG ACAAGAACTG TCCCATGTAC
    CAGAAGTTTT CACCCTTGGA AAGAGACCTG GACAACATGG AGCCTGCCGT CTATTCCTTA
    ACTATTGTCT CTGGTCAAAA CGTGTGCCCA AGTAGCAGCA CGGGAAGCCC ATGTATCGAA
    GTGGACGTGC TGGGCATGCC CCTGGACAGT TGCCACTTCC GCACAAAACC CATCCACCGG
    AACACTTTGA ACCCCATGTG GAATGAACAG TTTCTCTTCC GGGTTCACTT TGAAGATCTC
    ATATTCCTGC GTTTTGCAGT TGTGGAAAAC AATAGCTCAG CCATAACCGC TCAGAGAATC
    ATCCCCCTCA AGGCTTTAAA ACGAGGATAT CGACATCTTC AGCTGCGAAA CCTCCACAAT
    GAAGTCTTGG AAATCTCTAG TTTATTCATA AACAGCAGAA GGATGGAAGA AAATTCCTCC
    AGCAGTGCCA CGCCAGCCTC TTTGATGTTT AATACAGAAG AAAGAAAATG TTTGCAGACT
    CACAGAGTCA CAGTGCATGG GGTCCCAGGT CCAGAGCCCT TTACTGTTTT CTCCATAAGT
    GGAGGCACGA AGGCAAAGCA GCTTCTCCAA CAGATTCTAA CTACTGAACA AGACACCAAA
    CCTATTGTCA CAGACTATTT TTTGATGGAA GAAAAATATT TTATATCTAA AGAAAAGAAT
    GAATGCAGGA AACAACCATT TCAGAGAGTC ATCGGTCCAG AAGAAGAGAT CATGCAGATT
    TTAAGCAGCT GGTTTCCAGA GGAAGGATAT GTTGGCAGGA TTGTCTTAAA AACCCAGCAG
    GAAAACCTAG AAGAGAGAAG CATTGTTCAA GATGAGAAAG AGGTGATCTT GAGCTCGGAG
    GAGGAGAGCT TCTTTGTCCA AGTGCATGAC GTTTCTCCAG AGCAACCTCG GACGGTCATC
    AAAGCGCCCC GTGTCAGCAC CGCTCAGGAT GTCATTCAAC AGACCTTATG CAAAGCCAAA
    TATTCCTATA GCATCCTGAG TAACCCCAAC CCGAGTGACT ATGTGCTTCT GGAAGAGGTG
    GTGAAAGATG CTACCAAGAA GTCCTCCACC CCAAAGTCCT CCCAGCGGGT CCTTTTGGAC
    CAAGAGTGTG TGTTTCAAGC CCAGAGCAAG TGGAAAGGTG CTGGAAAATT CATCCTCAGG
    CTAAAGGAGC AGGTGCAGGC ATCCCGAGAA GATAAAAGAA AAGGCATTTC TTTCGCAAGT
    GAATTCAAGA AGTTCACCAA ATCAACTAAA CAGCCCCGAG GACTCACATC TTCTCAGGTC
    TTGGCCTCAG AAAGTGTCCA AAACAAGGAG GAGAAGCCCA CGGGCAGCTT GTCCTCCAGT
    GACACCACAG ACTCCCGACA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005637517.1
    CDS556..7479
    Translation

    Target ORF information:

    RefSeq Version XM_005637460.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris phospholipase C, epsilon 1 (PLCE1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005637460.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGACTTCTG AAGGAATGGC AGCTTCTGTT CTCACACCTG TGACTCAAAG AAAAGTGACA 
    TTTGCTCCAT CGGCTGTAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAG TGTTCCAAAG
    GCACATAGTG TCAAGCAGAG TGAGCAGACT TCTACCATCC CGTGGATGAA CAAGCTTAAA
    GAAGAATCTT CTGGAAGCAA CTTGCCCAAA ATTCTCTCCA TAGCAAGGGA GAAAATAGCA
    AGTGATGAGA ACAGCAATGA AGAGTGCTGG ACGGAAAGCA CACCAGTTTC TGTGAAAAAC
    CTTAACATTA ACCATAACAA CATCCTGACA AACCGCCAGT GCGTCCTTCC TCAGAGCCAG
    TCTTATGACA CGTGCAATTC TGTCATGGAG GAAGACCCAT GTTTAGAAAC TGGAATCTCA
    TCCTCACTGG AAAGAAAGGT ATTCCCTGGA ATTCAACTGG AAGTCAACAG ACCTCCCATG
    GACTTTAGGC CTCCAGGCCT AATGGACTTT AGTACCTTAG GAAGTCAGTC AGCCATTGTA
    GATACTGGCC AGGCGCACCC TGACAGCAAC AAAGCAGCAT TTCAAATCTT TAATTATAAA
    GTTGACAGAA GGATGTCTGA CACTTTCTGT ACCCTGTCAG GTGACTTGAT ATTGGATGAC
    TGTGGAAACT GTGTGCCACT GTCATCTGGT TTTGGCGGGG AGCAAAAGAA AAATTACGTG
    GCATATACCT GTAAACTCAT GGAATTGGCT GAAAACTGTG ATAATGAGAA TGGACAGCTG
    CAGTGTGATG ATTATGATGC CTTGGATGAC AAATACTTGT GCTTTGAAGA TTCTTGCCAA
    AGGGACAGTG TGGTATGTTC AAGTGACAGC TTTCGCAGGG AGGACCTAAC TAACAGTCCA
    CCTGCCAAGA CCTTTCTGAG CCATTTTGAA GACTTCCCTG ATAATGGTGA AGATGTAGAA
    GATTTTTTAA AAAACAAAAA GGAGAGATCC ACTTTGTTAG TGAGGAGATT TTGTAAAAAT
    GACAGAGAAG TAAAGAAATC TGTGTATACT GGGACAAGGG CAATCATGAG AACTCTGCCT
    TCTGGTCACA TTGGGCTGGA AGCTTACAGT TACATCGATC AGAAGAGAAG TGGTCCCTTA
    TTACCCCGTG GGAGAGTTCT GGAACAGCTG CCAGTGGTGG CAATTAGGCA AGATGGGAGC
    CAGTGCCTGT CAGAAGCCCA GTGGTATCGG ATCTACAATG CAGTGAGAAG GGAAGAAGAA
    ATAGAAAATA CCATTGGATC TCTTCTCCAT TACTTTACCA AGCTCCCAGC CTCCAAGACA
    GCCCATGAAA GGATTAGTGT TGGTCCATGC CTAAAGCAGT GTGTCCGAGA CACCATATGT
    GAGTATCGTG CCACCCTCCA GAGGACGTCC ATATCTCAGT ACATCACGGG TTCGCTCCTA
    GAAGCAACCA CATCTTTAGG AGCGAGAAGT GGCCTACTCA GTACTTTTGG AGGATCCACT
    GGACGAATGA TGCTAAAAGA ACGCCAGCCA GGCACCTCCA TGGCCAATTC CAGTGCCCTC
    CCTTCAAGTT CCGCTGGAAT CAGCAAGGAG CTGATTGACC TGCAGCCTCT CATTCAGTTC
    CCAGAGGAAG TCGCCAGCAT CCTGATGGAG CAGGAACAGA ACATTTACCG AAGGGTCTTA
    CCAGTTGACT ATCTTTACTT CTTGACCCGG GACTTGGGCA CTCCAGAATG CCAGACACCC
    CTGCCCTGCC TCAAAGCATC CATCTCAGCT TCAATTCTTA GCTCCCAGAA CGGAGAGCAC
    AATGCCCTGG AAGATCTTGT GATGAGATTT AATGAGGTGA GCTCCTGGGT GACATGGCTG
    ATCCTCACGG CGGGCTCCAT GGAGGAGAAG CGAGAAGTCT TCTCCTATTT GGTGCATGTG
    GCCAAATGCT GCTGGAACAT GGGCAACTAC AATGCTGTCA TGGAGTTCTT GGCTGGTCTC
    AGGTCAAGAA AAGTTTTAAA AATGTGGCAG TTTATGGACC AATCTGACCT CGAGACCATG
    AGGAGCCTGA AGGACGCCAT GGCTCAGCAT GAATCTTCCT GCGAATATCG GAAGGTGATA
    ACGAGGGCTC TGCACATACC TGGCTGTAAA GTGGTTCCAT TCTGTGGAGT GTTTCTGAAG
    GAGCTCTGTG AAGTACTGGA CGGAGCCTCT GGCCTCATGA AGCTTTGCCC GAGGTACAAT
    TCCCAAGAAG AAACTCTAGA GTTTGTAGCA GATTACAGTG GACAAGATAA TTTCTTACAA
    CGAGTGGGGC ATAATGGCTT AAAGAATTCA GAGAAGGAGT CCACTGTCAA CAGCATCTTT
    CAAATCATTA GGAGCTGCAG CCGAAGTCTG GAGGCTGAGG AAGAGGACAG CCCCAGTGAA
    GGGAACAGCT CTAGGAAAAA TTCTTTAAGG GACAAAGCCC GATGGCAATT TATAATCGGA
    GATTTGTTGG ATTCCGAAAA TGACATCTTT GAGCAATCCA AAGAATGGGA CTCTCCCAGC
    TCAGAAGAGC CTCAGAAAGC CTTTGACCAT GGGACAGAGC TGATCCCTTG GTACGTGCTG
    TCCATCCGAG CAGATGTGCA CCAGTTCCTG CTACAGGGCG CCACCGTCCT CCGCTACGAC
    CAGGACACGC ACCTCTCTGC CCGCTGCTTC CTCCAGCTTC AGCCTGACAA TAGCACCTTG
    ACATGGATAA AGCCCACAGC TGCCTCCCCG GCCAGTGCTA GAGCAAAACT CGGTGTGCTG
    AGCAACACGT CTGAGCCTGG CAAGTTCCCG TCACCAGGCA GTGCCGGACT AAGCGGCCTG
    GCCGAGGGGG TCCTGGATCT GTTTTCAGCA AAGGCTGTGT ACATGGGACA CCCTAGCATT
    GATATCCACA CTGTGTGTGT CCAGAACAAA CTCGGTAGCA TGTTTCTCTC AGAGACTGGC
    GTGACCCTGC TCTACGGGCT CCAGACCACA GACAATAGAT TATTGCACTT TGTGGCACCC
    AAACACACTG CTAAGATGCT CTTCAGTGGA TTGCTAGAAC TCACGAGGGC CGTGAGGAAG
    ATGAGAAAGT TCCCTGACCA GAGGCAGCAG TGGCTGCGGA AACAGTATGT TAGCCTCTAT
    CAGGAGGACG GACGGTATGA GGGCCCAACT TTGGCTCATG CTGTGGAGTT GTTTGGTGGC
    CGGCGATGGA GTACACGCAA TCCCAGCCCT GGGACATCTG CAAAGAGTGC TGAGAAGCCC
    AGTGTGCAGA GAAACAACAC CTTGGGCATA AGCACCACCA AGAAAAAGAA GAAAATCCTT
    ATAAGGGGTG AGAGTGGAGA GGCAGCCGAC GATGAGATGG CCACCCGGAA GGCCAAAATG
    CACAGGGAGT GTCGCAGCCG GAGTGGTTCT GACCCACAAG ACATGAATGA GCAAGAAGAA
    TCTGAGGCAA ATGCCATCAT GAGCCCTCCT AACACTCTGC CTTCCAGAAG AGCCCACTCT
    TTGACTACAG CTGGGTCCCC TAATTTGTCT GCCGGGACAT CATCTCCCAT CAGTGCTTGG
    AGCAGCAGCA GCTGGCATGG AAGGATCAAA GGAGGAATGA AGGGTTTTCA GAGCTTCATG
    GTTTCAGACA GTAACATGAG TTTTGTTGAA TTTGTTGAGC TGTTCAAATC ATTCAGTGTG
    AGGAGCCGCA AGGACCTGAA GGATCTCTTT GACATCTACG CTGTGCCCTG CAATCGGGCC
    GGCTCTGAGT CGGCCCCGCT CTACACCAAC CTAACAATAG ACGAAAACAC CAGCGATCTT
    CAGCCTGACC TAGATTTATT GACCAGAAAC GTCTCGGATT TGGGGTTGTT CATTAAGAGT
    AAACAGCAGC TGTCAGACAA CCAGAGGCAG ATTTCTGATG CCATTGCTGC TGCAAGTATC
    GTGACGAATG GCACTGGGAT CGAGAGCACA TCTTTGGGCA TCTTTGGGGT TGGCATACTC
    CAGCTCAATG ACTTCCTAGT CAACTGCCAG GGAGAACACT GCACTTACGA TGAAATCCTC
    AGCATCATCC AGAAGTTCGA GCCTAGCATT AGTATGTGTC ATCAGGGCCT ACTGTCCTTT
    GAAGGATTTG CAAGGTTTCT GATGGATAAA GATAATTTTG CCTCCAAAAA TGATGAGTCA
    CAGGAGAACA TTAAAGATTT GCAGCTGCCT CTCTCCTACT ATTACATTGA GTCTTCACAC
    AATACCTACC TCACAGGCCA CCAGCTCAAA GGAGAGTCAT CGGTAGAACT CTATAGCCAG
    GTCCTCCTGC AAGGCTGTAG AAGTGTAGAG TTGGACTGCT GGGATGGAGA TGACGGGATG
    CCCATCATCT ATCACGGACA CACACTAACA ACCAAGATCC CCTTCAAGGA GGTGGTTGAA
    GCCATCGACC GCAGTGCCTT CATCAACTCT GACCTGCCCA TCATCATATC GATTGAGAAC
    CACTGCTCGT TGCCTCAGCA ACGAAAAATG GCAGAAATTT TCAAGACTGT GTTTGGAGAA
    AAACTGGTGG CTAAATTCCT GTTTGAGAGC GATTTCTCAG ATGATCCAAT GCTTCCCTCA
    CCTGACCAAC TTAGGAGGAA AGTGCTTCTT AAAAATAAGA AGCTAAAAGC CCACCAGACA
    CCAGTGGATA TCTTAAAGCA AAAGGCTCAT CAGTTAGCAT CCATGCAAGC TCAGGCTTAT
    AATGGGGGAA ATGTCAACCC TCCACCTGCT AATAACGAGG AAGAGGAAGA TGAAGAGGAC
    GAATATGATT ATGACTATGA ATCCCTGTCT GATGCAGATG TCCTTACTGC TTCTCCTGCT
    CCTAACAGTC AGGAAGACAA CATTCTAGAA GACAGACCTG AAAATAAATC ATGTAATGAC
    AAGCTTCAGT TTGAGTATAA CGAAGAAATC CCCAAGAGGA TAAAGAAAGC AGATAATTCT
    GCTTTCAACA AAGGAAAGGT TTATGACATG GAACTGGGAG AAGAATTTTA TCTTCCTCAG
    AATAAAAAGG AAAGCAGACA GATTGCACCA GAGCTTTCTG ACCTTGTCAT CTACTGCCAA
    GCGGTAAAGT TTCCGGGGCT GTCAACTCTA AATGCATCTG GCTCTAACAG AGGAAAAGAG
    AGGAAAAGCA GGAAGTCCAT TTTTGGCAAC AATCCGGGCA GAATGAGCCC TGGGGAGACA
    GCATCGTTTA ACAAAGCATC TGGAAAAAGT TCCTGTGAAG GAATGCGACA GGCGTGGGAG
    GAATCTTCTT CCCCCCTCAA CCCAACCACA TCCCTCAGCG CTATCATTAG AACTCCCAAA
    TGTTACCATA TCTCATCGCT GAATGAAAAT GCCGCCAAAC GTCTGTGTCG CAGGTATTCT
    CAGAAACTGA TCCAGCACAC TGCCTGTCAG CTGCTGAGGA CTTACCCGGC TGCCACACGC
    ATCGACTCCT CCAACCCCAA TCCTCTCTTG TTCTGGCTCC ATGGGATACA GCTCGTGGCA
    CTCAACTACC AGACAGATGA TCTCCCTTTA CATTTAAATG CTGCCATGTT TGAGGCCAAC
    GGTGGCTGTG GTTATGTTTT GAAACCCCCC GTTCTGTGGG ACAAGAACTG TCCCATGTAC
    CAGAAGTTTT CACCCTTGGA AAGAGACCTG GACAACATGG AGCCTGCCGT CTATTCCTTA
    ACTATTGTCT CTGGTCAAAA CGTGTGCCCA AGTAGCAGCA CGGGAAGCCC ATGTATCGAA
    GTGGACGTGC TGGGCATGCC CCTGGACAGT TGCCACTTCC GCACAAAACC CATCCACCGG
    AACACTTTGA ACCCCATGTG GAATGAACAG TTTCTCTTCC GGGTTCACTT TGAAGATCTC
    ATATTCCTGC GTTTTGCAGT TGTGGAAAAC AATAGCTCAG CCATAACCGC TCAGAGAATC
    ATCCCCCTCA AGGCTTTAAA ACGAGGATAT CGACATCTTC AGCTGCGAAA CCTCCACAAT
    GAAGTCTTGG AAATCTCTAG TTTATTCATA AACAGCAGAA GGATGGAAGA AAATTCCTCC
    AGCAGTGCCA CGCCAGCCTC TTTGATGTTT AATACAGAAG AAAGAAAATG TTTGCAGACT
    CACAGAGTCA CAGTGCATGG GGTCCCAGGT CCAGAGCCCT TTACTGTTTT CTCCATAAGT
    GGAGGCACGA AGGCAAAGCA GCTTCTCCAA CAGATTCTAA CTACTGAACA AGACACCAAA
    CCTATTGTCA CAGACTATTT TTTGATGGAA GAAAAATATT TTATATCTAA AGAAAAGAAT
    GAATGCAGGA AACAACCATT TCAGAGAGTC ATCGGTCCAG AAGAAGAGAT CATGCAGATT
    TTAAGCAGCT GGTTTCCAGA GGAAGGATAT GTTGGCAGGA TTGTCTTAAA AACCCAGCAG
    GAAAACCTAG AAGAGAGAAG CATTGTTCAA GATGAGAAAG AGGTGATCTT GAGCTCGGAG
    GAGGAGAGCT TCTTTGTCCA AGTGCATGAC GTTTCTCCAG AGCAACCTCG GACGGTCATC
    AAAGCGCCCC GTGTCAGCAC CGCTCAGGAT GTCATTCAAC AGACCTTATG CAAAGCCAAA
    TATTCCTATA GCATCCTGAG TAACCCCAAC CCGAGTGACT ATGTGCTTCT GGAAGAGGTG
    GTGAAAGATG CTACCAAGAA GTCCTCCACC CCAAAGTCCT CCCAGCGGGT CCTTTTGGAC
    CAAGAGTGTG TGTTTCAAGC CCAGAGCAAG TGGAAAGGTG CTGGAAAATT CATCCTCAGG
    CTAAAGGAGC AGGTGCAGGC ATCCCGAGAA GATAAAAGAA AAGGCATTTC TTTCGCAAGT
    GAATTCAAGA AGTTCACCAA ATCAACTAAA CAGCCCCGAG GACTCACATC TTCTCAGGTC
    TTGGCCTCAG AAAGTGTCCA AAACAAGGAG GAGAAGCCCA CGGGCAGCTT GTCCTCCAGT
    GACACCACAG ACTCCCGACA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27698
    Clone ID Related Accession (Same CDS sequence) XM_022411589.1 , XM_005637461.2
    Accession Version XM_022411589.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6882bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006610.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGACTTCTG AAGGAATGGC AGCTTCTGTT CTCACACCTG TGACTCAAAG AAAAGTGACA 
    TTTGCTCCAT CGGCTGTAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAG TGTTCCAAAG
    GCACATAGTG TCAAGCAGAG TGAGCAGACT TCTACCATCC CGTGGATGAA CAAGCTTAAA
    GAAGAATCTT CTGGAAGCAA CTTGCCCAAA ATTCTCTCCA TAGCAAGGGA GAAAATAGCA
    AGTGATGAGA ACAGCAATGA AGAGTGCTGG ACGGAAAGCA CACCAGTTTC TGTGAAAAAC
    CTTAACATTA ACCATAACAA CATCCTGACA AACCGCCAGT GCGTCCTTCC TCAGAGCCAG
    TCTTATGACA CGTGCAATTC TGTCATGGAG GAAGACCCAT GTTTAGAAAC TGGAATCTCA
    TCCTCACTGG AAAGAAAGGT ATTCCCTGGA ATTCAACTGG AAGTCAACAG ACCTCCCATG
    GACTTTAGGC CTCCAGGCCT AATGGACTTT AGTACCTTAG GAAGTCAGTC AGCCATTGTA
    GATACTGGCC AGGCGCACCC TGACAGCAAC AAAGCAGCAT TTCAAATCTT TAATTATAAA
    GTTGACAGAA GGATGTCTGA CACTTTCTGT ACCCTGTCAG GTGACTTGAT ATTGGATGAC
    TGTGGAAACT GTGTGCCACT GTCATCTGGT TTTGGCGGGG AGCAAAAGAA AAATTACGTG
    GCATATACCT GTAAACTCAT GGAATTGGCT GAAAACTGTG ATAATGAGAA TGGACAGCTG
    CAGTGTGATG ATTATGATGC CTTGGATGAC AAATACTTGT GCTTTGAAGA TTCTTGCCAA
    AGGGACAGTG TGGTATGTTC AAGTGACAGC TTTCGCAGGG AGGACCTAAC TAACAGTCCA
    CCTGCCAAGA CCTTTCTGAG CCATTTTGAA GACTTCCCTG ATAATGGTGA AGATGTAGAA
    GATTTTTTAA AAAACAAAAA GGAGAGATCC ACTTTGTTAG TGAGGAGATT TTGTAAAAAT
    GACAGAGAAG TAAAGAAATC TGTGTATACT GGGACAAGGG CAATCATGAG AACTCTGCCT
    TCTGGTCACA TTGGGCTGGA AGCTTACAGT TACATCGATC AGAAGAGAAG TGGTCCCTTA
    TTACCCCGTG GGAGAGTTCT GGAACAGCTG CCAGTGGTGG CAATTAGGCA AGATGGGAGC
    CAGTGCCTGT CAGAAGCCCA GTGGTATCGG ATCTACAATG CAGTGAGAAG GGAAGAAGAA
    ATAGAAAATA CCATTGGATC TCTTCTCCAT TACTTTACCA AGCTCCCAGC CTCCAAGACA
    GCCCATGAAA GGATTAGTGT TGGTCCATGC CTAAAGCAGT GTGTCCGAGA CACCATATGT
    GAGTATCGTG CCACCCTCCA GAGGACGTCC ATATCTCAGT ACATCACGGG TTCGCTCCTA
    GAAGCAACCA CATCTTTAGG AGCGAGAAGT GGCCTACTCA GTACTTTTGG AGGATCCACT
    GGACGAATGA TGCTAAAAGA ACGCCAGCCA GGCACCTCCA TGGCCAATTC CAGTGCCCTC
    CCTTCAAGTT CCGCTGGAAT CAGCAAGGAG CTGATTGACC TGCAGCCTCT CATTCAGTTC
    CCAGAGGAAG TCGCCAGCAT CCTGATGGAG CAGGAACAGA ACATTTACCG AAGGGTCTTA
    CCAGTTGACT ATCTTTACTT CTTGACCCGG GACTTGGGCA CTCCAGAATG CCAGACACCC
    CTGCCCTGCC TCAAAGCATC CATCTCAGCT TCAATTCTTA GCTCCCAGAA CGGAGAGCAC
    AATGCCCTGG AAGATCTTGT GATGAGATTT AATGAGGTGA GCTCCTGGGT GACATGGCTG
    ATCCTCACGG CGGGCTCCAT GGAGGAGAAG CGAGAAGTCT TCTCCTATTT GGTGCATGTG
    GCCAAATGCT GCTGGAACAT GGGCAACTAC AATGCTGTCA TGGAGTTCTT GGCTGGTCTC
    AGGTCAAGAA AAGTTTTAAA AATGTGGCAG TTTATGGACC AATCTGACCT CGAGACCATG
    AGGAGCCTGA AGGACGCCAT GGCTCAGCAT GAATCTTCCT GCGAATATCG GAAGGTGATA
    ACGAGGGCTC TGCACATACC TGGCTGTAAA GTGGTTCCAT TCTGTGGAGT GTTTCTGAAG
    GAGCTCTGTG AAGTACTGGA CGGAGCCTCT GGCCTCATGA AGCTTTGCCC GAGGTACAAT
    TCCCAAGAAG AAACTCTAGA GTTTGTAGCA GATTACAGTG GACAAGATAA TTTCTTACAA
    CGAGTGGGGC ATAATGGCTT AAAGAATTCA GAGAAGGAGT CCACTGTCAA CAGCATCTTT
    CAAATCATTA GGAGCTGCAG CCGAAGTCTG GAGGCTGAGG AAGAGGACAG CCCCAGTGAA
    GGGAACAGCT CTAGGAAAAA TTCTTTAAGG GACAAAGCCC GATGGCAATT TATAATCGGA
    GATTTGTTGG ATTCCGAAAA TGACATCTTT GAGCAATCCA AAGAATGGGA CTCTCCCAGC
    TCAGAAGAGC CTCAGAAAGC CTTTGACCAT GGGACAGAGC TGATCCCTTG GTACGTGCTG
    TCCATCCGAG CAGATGTGCA CCAGTTCCTG CTACAGGGCG CCACCGTCCT CCGCTACGAC
    CAGGACACGC ACCTCTCTGC CCGCTGCTTC CTCCAGCTTC AGCCTGACAA TAGCACCTTG
    ACATGGATAA AGCCCACAGC TGCCTCCCCG GCCAGTGCTA GAGCAAAACT CGGTGTGCTG
    AGCAACACGT CTGAGCCTGG CAAGTTCCCG TCACCAGGCA GTGCCGGACT AAGCGGCCTG
    GCCGAGGGGG TCCTGGATCT GTTTTCAGCA AAGGCTGTGT ACATGGGACA CCCTAGCATT
    GATATCCACA CTGTGTGTGT CCAGAACAAA CTCGGTAGCA TGTTTCTCTC AGAGACTGGC
    GTGACCCTGC TCTACGGGCT CCAGACCACA GACAATAGAT TATTGCACTT TGTGGCACCC
    AAACACACTG CTAAGATGCT CTTCAGTGGA TTGCTAGAAC TCACGAGGGC CGTGAGGAAG
    ATGAGAAAGT TCCCTGACCA GAGGCAGCAG TGGCTGCGGA AACAGTATGT TAGCCTCTAT
    CAGGAGGACG GACGGTATGA GGGCCCAACT TTGGCTCATG CTGTGGAGTT GTTTGGTGGC
    CGGCGATGGA GTACACGCAA TCCCAGCCCT GGGACATCTG CAAAGAGTGC TGAGAAGCCC
    AGTGTGCAGA GAAACAACAC CTTGGGCATA AGCACCACCA AGAAAAAGAA GAAAATCCTT
    ATAAGGGGTG AGAGTGGAGA GGCAGCCGAC GATGAGATGG CCACCCGGAA GGCCAAAATG
    CACAGGGAGT GTCGCAGCCG GAGTGGTTCT GACCCACAAG ACATGAATGA GCAAGAAGAA
    TCTGAGGCAA ATGCCATCAT GAGCCCTCCT AACACTCTGC CTTCCAGAAG AGCCCACTCT
    TTGACTACAG CTGGGTCCCC TAATTTGTCT GCCGGGACAT CATCTCCCAT CAGTGCTTGG
    AGCAGCAGCA GCTGGCATGG AAGGATCAAA GGAGGAATGA AGGGTTTTCA GAGCTTCATG
    GTTTCAGACA GTAACATGAG TTTTGTTGAA TTTGTTGAGC TGTTCAAATC ATTCAGTGTG
    AGGAGCCGCA AGGACCTGAA GGATCTCTTT GACATCTACG CTGTGCCCTG CAATCGGGCC
    GGCTCTGAGT CGGCCCCGCT CTACACCAAC CTAACAATAG ACGAAAACAC CAGCGATCTT
    CAGCCTGACC TAGATTTATT GACCAGAAAC GTCTCGGATT TGGGGTTGTT CATTAAGAGT
    AAACAGCAGC TGTCAGACAA CCAGAGGCAG ATTTCTGATG CCATTGCTGC TGCAAGTATC
    GTGACGAATG GCACTGGGAT CGAGAGCACA TCTTTGGGCA TCTTTGGGGT TGGCATACTC
    CAGCTCAATG ACTTCCTAGT CAACTGCCAG GGAGAACACT GCACTTACGA TGAAATCCTC
    AGCATCATCC AGAAGTTCGA GCCTAGCATT AGTATGTGTC ATCAGGGCCT ACTGTCCTTT
    GAAGGATTTG CAAGGTTTCT GATGGATAAA GATAATTTTG CCTCCAAAAA TGATGAGTCA
    CAGGAGAACA TTAAAGATTT GCAGCTGCCT CTCTCCTACT ATTACATTGA GTCTTCACAC
    AATACCTACC TCACAGGCCA CCAGCTCAAA GGAGAGTCAT CGGTAGAACT CTATAGCCAG
    GTCCTCCTGC AAGGCTGTAG AAGTGTAGAG TTGGACTGCT GGGATGGAGA TGACGGGATG
    CCCATCATCT ATCACGGACA CACACTAACA ACCAAGATCC CCTTCAAGGA GGTGGTTGAA
    GCCATCGACC GCAGTGCCTT CATCAACTCT GACCTGCCCA TCATCATATC GATTGAGAAC
    CACTGCTCGT TGCCTCAGCA ACGAAAAATG GCAGAAATTT TCAAGACTGT GTTTGGAGAA
    AAACTGGTGG CTAAATTCCT GTTTGAGAGC GATTTCTCAG ATGATCCAAT GCTTCCCTCA
    CCTGACCAAC TTAGGAGGAA AGTGCTTCTT AAAAATAAGA AGCTAAAAGC CCACCAGACA
    CCAGTGGATA TCTTAAAGCA AAAGGCTCAT CAGTTAGCAT CCATGCAAGC TCAGGCTTAT
    AATGGGGGAA ATGTCAACCC TCCACCTGCT AATAACGAGG AAGAGGAAGA TGAAGAGGAC
    GAATATGATT ATGACTATGA ATCCCTGTCT GATGACAACA TTCTAGAAGA CAGACCTGAA
    AATAAATCAT GTAATGACAA GCTTCAGTTT GAGTATAACG AAGAAATCCC CAAGAGGATA
    AAGAAAGCAG ATAATTCTGC TTTCAACAAA GGAAAGGTTT ATGACATGGA ACTGGGAGAA
    GAATTTTATC TTCCTCAGAA TAAAAAGGAA AGCAGACAGA TTGCACCAGA GCTTTCTGAC
    CTTGTCATCT ACTGCCAAGC GGTAAAGTTT CCGGGGCTGT CAACTCTAAA TGCATCTGGC
    TCTAACAGAG GAAAAGAGAG GAAAAGCAGG AAGTCCATTT TTGGCAACAA TCCGGGCAGA
    ATGAGCCCTG GGGAGACAGC ATCGTTTAAC AAAGCATCTG GAAAAAGTTC CTGTGAAGGA
    ATGCGACAGG CGTGGGAGGA ATCTTCTTCC CCCCTCAACC CAACCACATC CCTCAGCGCT
    ATCATTAGAA CTCCCAAATG TTACCATATC TCATCGCTGA ATGAAAATGC CGCCAAACGT
    CTGTGTCGCA GGTATTCTCA GAAACTGATC CAGCACACTG CCTGTCAGCT GCTGAGGACT
    TACCCGGCTG CCACACGCAT CGACTCCTCC AACCCCAATC CTCTCTTGTT CTGGCTCCAT
    GGGATACAGC TCGTGGCACT CAACTACCAG ACAGATGATC TCCCTTTACA TTTAAATGCT
    GCCATGTTTG AGGCCAACGG TGGCTGTGGT TATGTTTTGA AACCCCCCGT TCTGTGGGAC
    AAGAACTGTC CCATGTACCA GAAGTTTTCA CCCTTGGAAA GAGACCTGGA CAACATGGAG
    CCTGCCGTCT ATTCCTTAAC TATTGTCTCT GGTCAAAACG TGTGCCCAAG TAGCAGCACG
    GGAAGCCCAT GTATCGAAGT GGACGTGCTG GGCATGCCCC TGGACAGTTG CCACTTCCGC
    ACAAAACCCA TCCACCGGAA CACTTTGAAC CCCATGTGGA ATGAACAGTT TCTCTTCCGG
    GTTCACTTTG AAGATCTCAT ATTCCTGCGT TTTGCAGTTG TGGAAAACAA TAGCTCAGCC
    ATAACCGCTC AGAGAATCAT CCCCCTCAAG GCTTTAAAAC GAGGATATCG ACATCTTCAG
    CTGCGAAACC TCCACAATGA AGTCTTGGAA ATCTCTAGTT TATTCATAAA CAGCAGAAGG
    ATGGAAGAAA ATTCCTCCAG CAGTGCCACG CCAGCCTCTT TGATGTTTAA TACAGAAGAA
    AGAAAATGTT TGCAGACTCA CAGAGTCACA GTGCATGGGG TCCCAGGTCC AGAGCCCTTT
    ACTGTTTTCT CCATAAGTGG AGGCACGAAG GCAAAGCAGC TTCTCCAACA GATTCTAACT
    ACTGAACAAG ACACCAAACC TATTGTCACA GACTATTTTT TGATGGAAGA AAAATATTTT
    ATATCTAAAG AAAAGAATGA ATGCAGGAAA CAACCATTTC AGAGAGTCAT CGGTCCAGAA
    GAAGAGATCA TGCAGATTTT AAGCAGCTGG TTTCCAGAGG AAGGATATGT TGGCAGGATT
    GTCTTAAAAA CCCAGCAGGA AAACCTAGAA GAGAGAAGCA TTGTTCAAGA TGAGAAAGAG
    GTGATCTTGA GCTCGGAGGA GGAGAGCTTC TTTGTCCAAG TGCATGACGT TTCTCCAGAG
    CAACCTCGGA CGGTCATCAA AGCGCCCCGT GTCAGCACCG CTCAGGATGT CATTCAACAG
    ACCTTATGCA AAGCCAAATA TTCCTATAGC ATCCTGAGTA ACCCCAACCC GAGTGACTAT
    GTGCTTCTGG AAGAGGTGGT GAAAGATGCT ACCAAGAAGT CCTCCACCCC AAAGTCCTCC
    CAGCGGGTCC TTTTGGACCA AGAGTGTGTG TTTCAAGCCC AGAGCAAGTG GAAAGGTGCT
    GGAAAATTCA TCCTCAGGCT AAAGGAGCAG GTGCAGGCAT CCCGAGAAGA TAAAAGAAAA
    GGCATTTCTT TCGCAAGTGA ATTCAAGAAG TTCACCAAAT CAACTAAACA GCCCCGAGGA
    CTCACATCTT CTCAGGTCTT GGCCTCAGAA AGTGTCCAAA ACAAGGAGGA GAAGCCCACG
    GGCAGCTTGT CCTCCAGTGA CACCACAGAC TCCCGACAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022267297.1
    CDS352..7233
    Translation

    Target ORF information:

    RefSeq Version XM_022411589.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris phospholipase C epsilon 1 (PLCE1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022411589.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGACTTCTG AAGGAATGGC AGCTTCTGTT CTCACACCTG TGACTCAAAG AAAAGTGACA 
    TTTGCTCCAT CGGCTGTAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAG TGTTCCAAAG
    GCACATAGTG TCAAGCAGAG TGAGCAGACT TCTACCATCC CGTGGATGAA CAAGCTTAAA
    GAAGAATCTT CTGGAAGCAA CTTGCCCAAA ATTCTCTCCA TAGCAAGGGA GAAAATAGCA
    AGTGATGAGA ACAGCAATGA AGAGTGCTGG ACGGAAAGCA CACCAGTTTC TGTGAAAAAC
    CTTAACATTA ACCATAACAA CATCCTGACA AACCGCCAGT GCGTCCTTCC TCAGAGCCAG
    TCTTATGACA CGTGCAATTC TGTCATGGAG GAAGACCCAT GTTTAGAAAC TGGAATCTCA
    TCCTCACTGG AAAGAAAGGT ATTCCCTGGA ATTCAACTGG AAGTCAACAG ACCTCCCATG
    GACTTTAGGC CTCCAGGCCT AATGGACTTT AGTACCTTAG GAAGTCAGTC AGCCATTGTA
    GATACTGGCC AGGCGCACCC TGACAGCAAC AAAGCAGCAT TTCAAATCTT TAATTATAAA
    GTTGACAGAA GGATGTCTGA CACTTTCTGT ACCCTGTCAG GTGACTTGAT ATTGGATGAC
    TGTGGAAACT GTGTGCCACT GTCATCTGGT TTTGGCGGGG AGCAAAAGAA AAATTACGTG
    GCATATACCT GTAAACTCAT GGAATTGGCT GAAAACTGTG ATAATGAGAA TGGACAGCTG
    CAGTGTGATG ATTATGATGC CTTGGATGAC AAATACTTGT GCTTTGAAGA TTCTTGCCAA
    AGGGACAGTG TGGTATGTTC AAGTGACAGC TTTCGCAGGG AGGACCTAAC TAACAGTCCA
    CCTGCCAAGA CCTTTCTGAG CCATTTTGAA GACTTCCCTG ATAATGGTGA AGATGTAGAA
    GATTTTTTAA AAAACAAAAA GGAGAGATCC ACTTTGTTAG TGAGGAGATT TTGTAAAAAT
    GACAGAGAAG TAAAGAAATC TGTGTATACT GGGACAAGGG CAATCATGAG AACTCTGCCT
    TCTGGTCACA TTGGGCTGGA AGCTTACAGT TACATCGATC AGAAGAGAAG TGGTCCCTTA
    TTACCCCGTG GGAGAGTTCT GGAACAGCTG CCAGTGGTGG CAATTAGGCA AGATGGGAGC
    CAGTGCCTGT CAGAAGCCCA GTGGTATCGG ATCTACAATG CAGTGAGAAG GGAAGAAGAA
    ATAGAAAATA CCATTGGATC TCTTCTCCAT TACTTTACCA AGCTCCCAGC CTCCAAGACA
    GCCCATGAAA GGATTAGTGT TGGTCCATGC CTAAAGCAGT GTGTCCGAGA CACCATATGT
    GAGTATCGTG CCACCCTCCA GAGGACGTCC ATATCTCAGT ACATCACGGG TTCGCTCCTA
    GAAGCAACCA CATCTTTAGG AGCGAGAAGT GGCCTACTCA GTACTTTTGG AGGATCCACT
    GGACGAATGA TGCTAAAAGA ACGCCAGCCA GGCACCTCCA TGGCCAATTC CAGTGCCCTC
    CCTTCAAGTT CCGCTGGAAT CAGCAAGGAG CTGATTGACC TGCAGCCTCT CATTCAGTTC
    CCAGAGGAAG TCGCCAGCAT CCTGATGGAG CAGGAACAGA ACATTTACCG AAGGGTCTTA
    CCAGTTGACT ATCTTTACTT CTTGACCCGG GACTTGGGCA CTCCAGAATG CCAGACACCC
    CTGCCCTGCC TCAAAGCATC CATCTCAGCT TCAATTCTTA GCTCCCAGAA CGGAGAGCAC
    AATGCCCTGG AAGATCTTGT GATGAGATTT AATGAGGTGA GCTCCTGGGT GACATGGCTG
    ATCCTCACGG CGGGCTCCAT GGAGGAGAAG CGAGAAGTCT TCTCCTATTT GGTGCATGTG
    GCCAAATGCT GCTGGAACAT GGGCAACTAC AATGCTGTCA TGGAGTTCTT GGCTGGTCTC
    AGGTCAAGAA AAGTTTTAAA AATGTGGCAG TTTATGGACC AATCTGACCT CGAGACCATG
    AGGAGCCTGA AGGACGCCAT GGCTCAGCAT GAATCTTCCT GCGAATATCG GAAGGTGATA
    ACGAGGGCTC TGCACATACC TGGCTGTAAA GTGGTTCCAT TCTGTGGAGT GTTTCTGAAG
    GAGCTCTGTG AAGTACTGGA CGGAGCCTCT GGCCTCATGA AGCTTTGCCC GAGGTACAAT
    TCCCAAGAAG AAACTCTAGA GTTTGTAGCA GATTACAGTG GACAAGATAA TTTCTTACAA
    CGAGTGGGGC ATAATGGCTT AAAGAATTCA GAGAAGGAGT CCACTGTCAA CAGCATCTTT
    CAAATCATTA GGAGCTGCAG CCGAAGTCTG GAGGCTGAGG AAGAGGACAG CCCCAGTGAA
    GGGAACAGCT CTAGGAAAAA TTCTTTAAGG GACAAAGCCC GATGGCAATT TATAATCGGA
    GATTTGTTGG ATTCCGAAAA TGACATCTTT GAGCAATCCA AAGAATGGGA CTCTCCCAGC
    TCAGAAGAGC CTCAGAAAGC CTTTGACCAT GGGACAGAGC TGATCCCTTG GTACGTGCTG
    TCCATCCGAG CAGATGTGCA CCAGTTCCTG CTACAGGGCG CCACCGTCCT CCGCTACGAC
    CAGGACACGC ACCTCTCTGC CCGCTGCTTC CTCCAGCTTC AGCCTGACAA TAGCACCTTG
    ACATGGATAA AGCCCACAGC TGCCTCCCCG GCCAGTGCTA GAGCAAAACT CGGTGTGCTG
    AGCAACACGT CTGAGCCTGG CAAGTTCCCG TCACCAGGCA GTGCCGGACT AAGCGGCCTG
    GCCGAGGGGG TCCTGGATCT GTTTTCAGCA AAGGCTGTGT ACATGGGACA CCCTAGCATT
    GATATCCACA CTGTGTGTGT CCAGAACAAA CTCGGTAGCA TGTTTCTCTC AGAGACTGGC
    GTGACCCTGC TCTACGGGCT CCAGACCACA GACAATAGAT TATTGCACTT TGTGGCACCC
    AAACACACTG CTAAGATGCT CTTCAGTGGA TTGCTAGAAC TCACGAGGGC CGTGAGGAAG
    ATGAGAAAGT TCCCTGACCA GAGGCAGCAG TGGCTGCGGA AACAGTATGT TAGCCTCTAT
    CAGGAGGACG GACGGTATGA GGGCCCAACT TTGGCTCATG CTGTGGAGTT GTTTGGTGGC
    CGGCGATGGA GTACACGCAA TCCCAGCCCT GGGACATCTG CAAAGAGTGC TGAGAAGCCC
    AGTGTGCAGA GAAACAACAC CTTGGGCATA AGCACCACCA AGAAAAAGAA GAAAATCCTT
    ATAAGGGGTG AGAGTGGAGA GGCAGCCGAC GATGAGATGG CCACCCGGAA GGCCAAAATG
    CACAGGGAGT GTCGCAGCCG GAGTGGTTCT GACCCACAAG ACATGAATGA GCAAGAAGAA
    TCTGAGGCAA ATGCCATCAT GAGCCCTCCT AACACTCTGC CTTCCAGAAG AGCCCACTCT
    TTGACTACAG CTGGGTCCCC TAATTTGTCT GCCGGGACAT CATCTCCCAT CAGTGCTTGG
    AGCAGCAGCA GCTGGCATGG AAGGATCAAA GGAGGAATGA AGGGTTTTCA GAGCTTCATG
    GTTTCAGACA GTAACATGAG TTTTGTTGAA TTTGTTGAGC TGTTCAAATC ATTCAGTGTG
    AGGAGCCGCA AGGACCTGAA GGATCTCTTT GACATCTACG CTGTGCCCTG CAATCGGGCC
    GGCTCTGAGT CGGCCCCGCT CTACACCAAC CTAACAATAG ACGAAAACAC CAGCGATCTT
    CAGCCTGACC TAGATTTATT GACCAGAAAC GTCTCGGATT TGGGGTTGTT CATTAAGAGT
    AAACAGCAGC TGTCAGACAA CCAGAGGCAG ATTTCTGATG CCATTGCTGC TGCAAGTATC
    GTGACGAATG GCACTGGGAT CGAGAGCACA TCTTTGGGCA TCTTTGGGGT TGGCATACTC
    CAGCTCAATG ACTTCCTAGT CAACTGCCAG GGAGAACACT GCACTTACGA TGAAATCCTC
    AGCATCATCC AGAAGTTCGA GCCTAGCATT AGTATGTGTC ATCAGGGCCT ACTGTCCTTT
    GAAGGATTTG CAAGGTTTCT GATGGATAAA GATAATTTTG CCTCCAAAAA TGATGAGTCA
    CAGGAGAACA TTAAAGATTT GCAGCTGCCT CTCTCCTACT ATTACATTGA GTCTTCACAC
    AATACCTACC TCACAGGCCA CCAGCTCAAA GGAGAGTCAT CGGTAGAACT CTATAGCCAG
    GTCCTCCTGC AAGGCTGTAG AAGTGTAGAG TTGGACTGCT GGGATGGAGA TGACGGGATG
    CCCATCATCT ATCACGGACA CACACTAACA ACCAAGATCC CCTTCAAGGA GGTGGTTGAA
    GCCATCGACC GCAGTGCCTT CATCAACTCT GACCTGCCCA TCATCATATC GATTGAGAAC
    CACTGCTCGT TGCCTCAGCA ACGAAAAATG GCAGAAATTT TCAAGACTGT GTTTGGAGAA
    AAACTGGTGG CTAAATTCCT GTTTGAGAGC GATTTCTCAG ATGATCCAAT GCTTCCCTCA
    CCTGACCAAC TTAGGAGGAA AGTGCTTCTT AAAAATAAGA AGCTAAAAGC CCACCAGACA
    CCAGTGGATA TCTTAAAGCA AAAGGCTCAT CAGTTAGCAT CCATGCAAGC TCAGGCTTAT
    AATGGGGGAA ATGTCAACCC TCCACCTGCT AATAACGAGG AAGAGGAAGA TGAAGAGGAC
    GAATATGATT ATGACTATGA ATCCCTGTCT GATGACAACA TTCTAGAAGA CAGACCTGAA
    AATAAATCAT GTAATGACAA GCTTCAGTTT GAGTATAACG AAGAAATCCC CAAGAGGATA
    AAGAAAGCAG ATAATTCTGC TTTCAACAAA GGAAAGGTTT ATGACATGGA ACTGGGAGAA
    GAATTTTATC TTCCTCAGAA TAAAAAGGAA AGCAGACAGA TTGCACCAGA GCTTTCTGAC
    CTTGTCATCT ACTGCCAAGC GGTAAAGTTT CCGGGGCTGT CAACTCTAAA TGCATCTGGC
    TCTAACAGAG GAAAAGAGAG GAAAAGCAGG AAGTCCATTT TTGGCAACAA TCCGGGCAGA
    ATGAGCCCTG GGGAGACAGC ATCGTTTAAC AAAGCATCTG GAAAAAGTTC CTGTGAAGGA
    ATGCGACAGG CGTGGGAGGA ATCTTCTTCC CCCCTCAACC CAACCACATC CCTCAGCGCT
    ATCATTAGAA CTCCCAAATG TTACCATATC TCATCGCTGA ATGAAAATGC CGCCAAACGT
    CTGTGTCGCA GGTATTCTCA GAAACTGATC CAGCACACTG CCTGTCAGCT GCTGAGGACT
    TACCCGGCTG CCACACGCAT CGACTCCTCC AACCCCAATC CTCTCTTGTT CTGGCTCCAT
    GGGATACAGC TCGTGGCACT CAACTACCAG ACAGATGATC TCCCTTTACA TTTAAATGCT
    GCCATGTTTG AGGCCAACGG TGGCTGTGGT TATGTTTTGA AACCCCCCGT TCTGTGGGAC
    AAGAACTGTC CCATGTACCA GAAGTTTTCA CCCTTGGAAA GAGACCTGGA CAACATGGAG
    CCTGCCGTCT ATTCCTTAAC TATTGTCTCT GGTCAAAACG TGTGCCCAAG TAGCAGCACG
    GGAAGCCCAT GTATCGAAGT GGACGTGCTG GGCATGCCCC TGGACAGTTG CCACTTCCGC
    ACAAAACCCA TCCACCGGAA CACTTTGAAC CCCATGTGGA ATGAACAGTT TCTCTTCCGG
    GTTCACTTTG AAGATCTCAT ATTCCTGCGT TTTGCAGTTG TGGAAAACAA TAGCTCAGCC
    ATAACCGCTC AGAGAATCAT CCCCCTCAAG GCTTTAAAAC GAGGATATCG ACATCTTCAG
    CTGCGAAACC TCCACAATGA AGTCTTGGAA ATCTCTAGTT TATTCATAAA CAGCAGAAGG
    ATGGAAGAAA ATTCCTCCAG CAGTGCCACG CCAGCCTCTT TGATGTTTAA TACAGAAGAA
    AGAAAATGTT TGCAGACTCA CAGAGTCACA GTGCATGGGG TCCCAGGTCC AGAGCCCTTT
    ACTGTTTTCT CCATAAGTGG AGGCACGAAG GCAAAGCAGC TTCTCCAACA GATTCTAACT
    ACTGAACAAG ACACCAAACC TATTGTCACA GACTATTTTT TGATGGAAGA AAAATATTTT
    ATATCTAAAG AAAAGAATGA ATGCAGGAAA CAACCATTTC AGAGAGTCAT CGGTCCAGAA
    GAAGAGATCA TGCAGATTTT AAGCAGCTGG TTTCCAGAGG AAGGATATGT TGGCAGGATT
    GTCTTAAAAA CCCAGCAGGA AAACCTAGAA GAGAGAAGCA TTGTTCAAGA TGAGAAAGAG
    GTGATCTTGA GCTCGGAGGA GGAGAGCTTC TTTGTCCAAG TGCATGACGT TTCTCCAGAG
    CAACCTCGGA CGGTCATCAA AGCGCCCCGT GTCAGCACCG CTCAGGATGT CATTCAACAG
    ACCTTATGCA AAGCCAAATA TTCCTATAGC ATCCTGAGTA ACCCCAACCC GAGTGACTAT
    GTGCTTCTGG AAGAGGTGGT GAAAGATGCT ACCAAGAAGT CCTCCACCCC AAAGTCCTCC
    CAGCGGGTCC TTTTGGACCA AGAGTGTGTG TTTCAAGCCC AGAGCAAGTG GAAAGGTGCT
    GGAAAATTCA TCCTCAGGCT AAAGGAGCAG GTGCAGGCAT CCCGAGAAGA TAAAAGAAAA
    GGCATTTCTT TCGCAAGTGA ATTCAAGAAG TTCACCAAAT CAACTAAACA GCCCCGAGGA
    CTCACATCTT CTCAGGTCTT GGCCTCAGAA AGTGTCCAAA ACAAGGAGGA GAAGCCCACG
    GGCAGCTTGT CCTCCAGTGA CACCACAGAC TCCCGACAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27698
    Clone ID Related Accession (Same CDS sequence) XM_022411589.1 , XM_005637461.2
    Accession Version XM_005637461.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6882bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005637461.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
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    301
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGACTTCTG AAGGAATGGC AGCTTCTGTT CTCACACCTG TGACTCAAAG AAAAGTGACA 
    TTTGCTCCAT CGGCTGTAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAG TGTTCCAAAG
    GCACATAGTG TCAAGCAGAG TGAGCAGACT TCTACCATCC CGTGGATGAA CAAGCTTAAA
    GAAGAATCTT CTGGAAGCAA CTTGCCCAAA ATTCTCTCCA TAGCAAGGGA GAAAATAGCA
    AGTGATGAGA ACAGCAATGA AGAGTGCTGG ACGGAAAGCA CACCAGTTTC TGTGAAAAAC
    CTTAACATTA ACCATAACAA CATCCTGACA AACCGCCAGT GCGTCCTTCC TCAGAGCCAG
    TCTTATGACA CGTGCAATTC TGTCATGGAG GAAGACCCAT GTTTAGAAAC TGGAATCTCA
    TCCTCACTGG AAAGAAAGGT ATTCCCTGGA ATTCAACTGG AAGTCAACAG ACCTCCCATG
    GACTTTAGGC CTCCAGGCCT AATGGACTTT AGTACCTTAG GAAGTCAGTC AGCCATTGTA
    GATACTGGCC AGGCGCACCC TGACAGCAAC AAAGCAGCAT TTCAAATCTT TAATTATAAA
    GTTGACAGAA GGATGTCTGA CACTTTCTGT ACCCTGTCAG GTGACTTGAT ATTGGATGAC
    TGTGGAAACT GTGTGCCACT GTCATCTGGT TTTGGCGGGG AGCAAAAGAA AAATTACGTG
    GCATATACCT GTAAACTCAT GGAATTGGCT GAAAACTGTG ATAATGAGAA TGGACAGCTG
    CAGTGTGATG ATTATGATGC CTTGGATGAC AAATACTTGT GCTTTGAAGA TTCTTGCCAA
    AGGGACAGTG TGGTATGTTC AAGTGACAGC TTTCGCAGGG AGGACCTAAC TAACAGTCCA
    CCTGCCAAGA CCTTTCTGAG CCATTTTGAA GACTTCCCTG ATAATGGTGA AGATGTAGAA
    GATTTTTTAA AAAACAAAAA GGAGAGATCC ACTTTGTTAG TGAGGAGATT TTGTAAAAAT
    GACAGAGAAG TAAAGAAATC TGTGTATACT GGGACAAGGG CAATCATGAG AACTCTGCCT
    TCTGGTCACA TTGGGCTGGA AGCTTACAGT TACATCGATC AGAAGAGAAG TGGTCCCTTA
    TTACCCCGTG GGAGAGTTCT GGAACAGCTG CCAGTGGTGG CAATTAGGCA AGATGGGAGC
    CAGTGCCTGT CAGAAGCCCA GTGGTATCGG ATCTACAATG CAGTGAGAAG GGAAGAAGAA
    ATAGAAAATA CCATTGGATC TCTTCTCCAT TACTTTACCA AGCTCCCAGC CTCCAAGACA
    GCCCATGAAA GGATTAGTGT TGGTCCATGC CTAAAGCAGT GTGTCCGAGA CACCATATGT
    GAGTATCGTG CCACCCTCCA GAGGACGTCC ATATCTCAGT ACATCACGGG TTCGCTCCTA
    GAAGCAACCA CATCTTTAGG AGCGAGAAGT GGCCTACTCA GTACTTTTGG AGGATCCACT
    GGACGAATGA TGCTAAAAGA ACGCCAGCCA GGCACCTCCA TGGCCAATTC CAGTGCCCTC
    CCTTCAAGTT CCGCTGGAAT CAGCAAGGAG CTGATTGACC TGCAGCCTCT CATTCAGTTC
    CCAGAGGAAG TCGCCAGCAT CCTGATGGAG CAGGAACAGA ACATTTACCG AAGGGTCTTA
    CCAGTTGACT ATCTTTACTT CTTGACCCGG GACTTGGGCA CTCCAGAATG CCAGACACCC
    CTGCCCTGCC TCAAAGCATC CATCTCAGCT TCAATTCTTA GCTCCCAGAA CGGAGAGCAC
    AATGCCCTGG AAGATCTTGT GATGAGATTT AATGAGGTGA GCTCCTGGGT GACATGGCTG
    ATCCTCACGG CGGGCTCCAT GGAGGAGAAG CGAGAAGTCT TCTCCTATTT GGTGCATGTG
    GCCAAATGCT GCTGGAACAT GGGCAACTAC AATGCTGTCA TGGAGTTCTT GGCTGGTCTC
    AGGTCAAGAA AAGTTTTAAA AATGTGGCAG TTTATGGACC AATCTGACCT CGAGACCATG
    AGGAGCCTGA AGGACGCCAT GGCTCAGCAT GAATCTTCCT GCGAATATCG GAAGGTGATA
    ACGAGGGCTC TGCACATACC TGGCTGTAAA GTGGTTCCAT TCTGTGGAGT GTTTCTGAAG
    GAGCTCTGTG AAGTACTGGA CGGAGCCTCT GGCCTCATGA AGCTTTGCCC GAGGTACAAT
    TCCCAAGAAG AAACTCTAGA GTTTGTAGCA GATTACAGTG GACAAGATAA TTTCTTACAA
    CGAGTGGGGC ATAATGGCTT AAAGAATTCA GAGAAGGAGT CCACTGTCAA CAGCATCTTT
    CAAATCATTA GGAGCTGCAG CCGAAGTCTG GAGGCTGAGG AAGAGGACAG CCCCAGTGAA
    GGGAACAGCT CTAGGAAAAA TTCTTTAAGG GACAAAGCCC GATGGCAATT TATAATCGGA
    GATTTGTTGG ATTCCGAAAA TGACATCTTT GAGCAATCCA AAGAATGGGA CTCTCCCAGC
    TCAGAAGAGC CTCAGAAAGC CTTTGACCAT GGGACAGAGC TGATCCCTTG GTACGTGCTG
    TCCATCCGAG CAGATGTGCA CCAGTTCCTG CTACAGGGCG CCACCGTCCT CCGCTACGAC
    CAGGACACGC ACCTCTCTGC CCGCTGCTTC CTCCAGCTTC AGCCTGACAA TAGCACCTTG
    ACATGGATAA AGCCCACAGC TGCCTCCCCG GCCAGTGCTA GAGCAAAACT CGGTGTGCTG
    AGCAACACGT CTGAGCCTGG CAAGTTCCCG TCACCAGGCA GTGCCGGACT AAGCGGCCTG
    GCCGAGGGGG TCCTGGATCT GTTTTCAGCA AAGGCTGTGT ACATGGGACA CCCTAGCATT
    GATATCCACA CTGTGTGTGT CCAGAACAAA CTCGGTAGCA TGTTTCTCTC AGAGACTGGC
    GTGACCCTGC TCTACGGGCT CCAGACCACA GACAATAGAT TATTGCACTT TGTGGCACCC
    AAACACACTG CTAAGATGCT CTTCAGTGGA TTGCTAGAAC TCACGAGGGC CGTGAGGAAG
    ATGAGAAAGT TCCCTGACCA GAGGCAGCAG TGGCTGCGGA AACAGTATGT TAGCCTCTAT
    CAGGAGGACG GACGGTATGA GGGCCCAACT TTGGCTCATG CTGTGGAGTT GTTTGGTGGC
    CGGCGATGGA GTACACGCAA TCCCAGCCCT GGGACATCTG CAAAGAGTGC TGAGAAGCCC
    AGTGTGCAGA GAAACAACAC CTTGGGCATA AGCACCACCA AGAAAAAGAA GAAAATCCTT
    ATAAGGGGTG AGAGTGGAGA GGCAGCCGAC GATGAGATGG CCACCCGGAA GGCCAAAATG
    CACAGGGAGT GTCGCAGCCG GAGTGGTTCT GACCCACAAG ACATGAATGA GCAAGAAGAA
    TCTGAGGCAA ATGCCATCAT GAGCCCTCCT AACACTCTGC CTTCCAGAAG AGCCCACTCT
    TTGACTACAG CTGGGTCCCC TAATTTGTCT GCCGGGACAT CATCTCCCAT CAGTGCTTGG
    AGCAGCAGCA GCTGGCATGG AAGGATCAAA GGAGGAATGA AGGGTTTTCA GAGCTTCATG
    GTTTCAGACA GTAACATGAG TTTTGTTGAA TTTGTTGAGC TGTTCAAATC ATTCAGTGTG
    AGGAGCCGCA AGGACCTGAA GGATCTCTTT GACATCTACG CTGTGCCCTG CAATCGGGCC
    GGCTCTGAGT CGGCCCCGCT CTACACCAAC CTAACAATAG ACGAAAACAC CAGCGATCTT
    CAGCCTGACC TAGATTTATT GACCAGAAAC GTCTCGGATT TGGGGTTGTT CATTAAGAGT
    AAACAGCAGC TGTCAGACAA CCAGAGGCAG ATTTCTGATG CCATTGCTGC TGCAAGTATC
    GTGACGAATG GCACTGGGAT CGAGAGCACA TCTTTGGGCA TCTTTGGGGT TGGCATACTC
    CAGCTCAATG ACTTCCTAGT CAACTGCCAG GGAGAACACT GCACTTACGA TGAAATCCTC
    AGCATCATCC AGAAGTTCGA GCCTAGCATT AGTATGTGTC ATCAGGGCCT ACTGTCCTTT
    GAAGGATTTG CAAGGTTTCT GATGGATAAA GATAATTTTG CCTCCAAAAA TGATGAGTCA
    CAGGAGAACA TTAAAGATTT GCAGCTGCCT CTCTCCTACT ATTACATTGA GTCTTCACAC
    AATACCTACC TCACAGGCCA CCAGCTCAAA GGAGAGTCAT CGGTAGAACT CTATAGCCAG
    GTCCTCCTGC AAGGCTGTAG AAGTGTAGAG TTGGACTGCT GGGATGGAGA TGACGGGATG
    CCCATCATCT ATCACGGACA CACACTAACA ACCAAGATCC CCTTCAAGGA GGTGGTTGAA
    GCCATCGACC GCAGTGCCTT CATCAACTCT GACCTGCCCA TCATCATATC GATTGAGAAC
    CACTGCTCGT TGCCTCAGCA ACGAAAAATG GCAGAAATTT TCAAGACTGT GTTTGGAGAA
    AAACTGGTGG CTAAATTCCT GTTTGAGAGC GATTTCTCAG ATGATCCAAT GCTTCCCTCA
    CCTGACCAAC TTAGGAGGAA AGTGCTTCTT AAAAATAAGA AGCTAAAAGC CCACCAGACA
    CCAGTGGATA TCTTAAAGCA AAAGGCTCAT CAGTTAGCAT CCATGCAAGC TCAGGCTTAT
    AATGGGGGAA ATGTCAACCC TCCACCTGCT AATAACGAGG AAGAGGAAGA TGAAGAGGAC
    GAATATGATT ATGACTATGA ATCCCTGTCT GATGACAACA TTCTAGAAGA CAGACCTGAA
    AATAAATCAT GTAATGACAA GCTTCAGTTT GAGTATAACG AAGAAATCCC CAAGAGGATA
    AAGAAAGCAG ATAATTCTGC TTTCAACAAA GGAAAGGTTT ATGACATGGA ACTGGGAGAA
    GAATTTTATC TTCCTCAGAA TAAAAAGGAA AGCAGACAGA TTGCACCAGA GCTTTCTGAC
    CTTGTCATCT ACTGCCAAGC GGTAAAGTTT CCGGGGCTGT CAACTCTAAA TGCATCTGGC
    TCTAACAGAG GAAAAGAGAG GAAAAGCAGG AAGTCCATTT TTGGCAACAA TCCGGGCAGA
    ATGAGCCCTG GGGAGACAGC ATCGTTTAAC AAAGCATCTG GAAAAAGTTC CTGTGAAGGA
    ATGCGACAGG CGTGGGAGGA ATCTTCTTCC CCCCTCAACC CAACCACATC CCTCAGCGCT
    ATCATTAGAA CTCCCAAATG TTACCATATC TCATCGCTGA ATGAAAATGC CGCCAAACGT
    CTGTGTCGCA GGTATTCTCA GAAACTGATC CAGCACACTG CCTGTCAGCT GCTGAGGACT
    TACCCGGCTG CCACACGCAT CGACTCCTCC AACCCCAATC CTCTCTTGTT CTGGCTCCAT
    GGGATACAGC TCGTGGCACT CAACTACCAG ACAGATGATC TCCCTTTACA TTTAAATGCT
    GCCATGTTTG AGGCCAACGG TGGCTGTGGT TATGTTTTGA AACCCCCCGT TCTGTGGGAC
    AAGAACTGTC CCATGTACCA GAAGTTTTCA CCCTTGGAAA GAGACCTGGA CAACATGGAG
    CCTGCCGTCT ATTCCTTAAC TATTGTCTCT GGTCAAAACG TGTGCCCAAG TAGCAGCACG
    GGAAGCCCAT GTATCGAAGT GGACGTGCTG GGCATGCCCC TGGACAGTTG CCACTTCCGC
    ACAAAACCCA TCCACCGGAA CACTTTGAAC CCCATGTGGA ATGAACAGTT TCTCTTCCGG
    GTTCACTTTG AAGATCTCAT ATTCCTGCGT TTTGCAGTTG TGGAAAACAA TAGCTCAGCC
    ATAACCGCTC AGAGAATCAT CCCCCTCAAG GCTTTAAAAC GAGGATATCG ACATCTTCAG
    CTGCGAAACC TCCACAATGA AGTCTTGGAA ATCTCTAGTT TATTCATAAA CAGCAGAAGG
    ATGGAAGAAA ATTCCTCCAG CAGTGCCACG CCAGCCTCTT TGATGTTTAA TACAGAAGAA
    AGAAAATGTT TGCAGACTCA CAGAGTCACA GTGCATGGGG TCCCAGGTCC AGAGCCCTTT
    ACTGTTTTCT CCATAAGTGG AGGCACGAAG GCAAAGCAGC TTCTCCAACA GATTCTAACT
    ACTGAACAAG ACACCAAACC TATTGTCACA GACTATTTTT TGATGGAAGA AAAATATTTT
    ATATCTAAAG AAAAGAATGA ATGCAGGAAA CAACCATTTC AGAGAGTCAT CGGTCCAGAA
    GAAGAGATCA TGCAGATTTT AAGCAGCTGG TTTCCAGAGG AAGGATATGT TGGCAGGATT
    GTCTTAAAAA CCCAGCAGGA AAACCTAGAA GAGAGAAGCA TTGTTCAAGA TGAGAAAGAG
    GTGATCTTGA GCTCGGAGGA GGAGAGCTTC TTTGTCCAAG TGCATGACGT TTCTCCAGAG
    CAACCTCGGA CGGTCATCAA AGCGCCCCGT GTCAGCACCG CTCAGGATGT CATTCAACAG
    ACCTTATGCA AAGCCAAATA TTCCTATAGC ATCCTGAGTA ACCCCAACCC GAGTGACTAT
    GTGCTTCTGG AAGAGGTGGT GAAAGATGCT ACCAAGAAGT CCTCCACCCC AAAGTCCTCC
    CAGCGGGTCC TTTTGGACCA AGAGTGTGTG TTTCAAGCCC AGAGCAAGTG GAAAGGTGCT
    GGAAAATTCA TCCTCAGGCT AAAGGAGCAG GTGCAGGCAT CCCGAGAAGA TAAAAGAAAA
    GGCATTTCTT TCGCAAGTGA ATTCAAGAAG TTCACCAAAT CAACTAAACA GCCCCGAGGA
    CTCACATCTT CTCAGGTCTT GGCCTCAGAA AGTGTCCAAA ACAAGGAGGA GAAGCCCACG
    GGCAGCTTGT CCTCCAGTGA CACCACAGAC TCCCGACAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005637518.1
    CDS555..7436
    Translation

    Target ORF information:

    RefSeq Version XM_005637461.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris phospholipase C, epsilon 1 (PLCE1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005637461.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGACTTCTG AAGGAATGGC AGCTTCTGTT CTCACACCTG TGACTCAAAG AAAAGTGACA 
    TTTGCTCCAT CGGCTGTAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAG TGTTCCAAAG
    GCACATAGTG TCAAGCAGAG TGAGCAGACT TCTACCATCC CGTGGATGAA CAAGCTTAAA
    GAAGAATCTT CTGGAAGCAA CTTGCCCAAA ATTCTCTCCA TAGCAAGGGA GAAAATAGCA
    AGTGATGAGA ACAGCAATGA AGAGTGCTGG ACGGAAAGCA CACCAGTTTC TGTGAAAAAC
    CTTAACATTA ACCATAACAA CATCCTGACA AACCGCCAGT GCGTCCTTCC TCAGAGCCAG
    TCTTATGACA CGTGCAATTC TGTCATGGAG GAAGACCCAT GTTTAGAAAC TGGAATCTCA
    TCCTCACTGG AAAGAAAGGT ATTCCCTGGA ATTCAACTGG AAGTCAACAG ACCTCCCATG
    GACTTTAGGC CTCCAGGCCT AATGGACTTT AGTACCTTAG GAAGTCAGTC AGCCATTGTA
    GATACTGGCC AGGCGCACCC TGACAGCAAC AAAGCAGCAT TTCAAATCTT TAATTATAAA
    GTTGACAGAA GGATGTCTGA CACTTTCTGT ACCCTGTCAG GTGACTTGAT ATTGGATGAC
    TGTGGAAACT GTGTGCCACT GTCATCTGGT TTTGGCGGGG AGCAAAAGAA AAATTACGTG
    GCATATACCT GTAAACTCAT GGAATTGGCT GAAAACTGTG ATAATGAGAA TGGACAGCTG
    CAGTGTGATG ATTATGATGC CTTGGATGAC AAATACTTGT GCTTTGAAGA TTCTTGCCAA
    AGGGACAGTG TGGTATGTTC AAGTGACAGC TTTCGCAGGG AGGACCTAAC TAACAGTCCA
    CCTGCCAAGA CCTTTCTGAG CCATTTTGAA GACTTCCCTG ATAATGGTGA AGATGTAGAA
    GATTTTTTAA AAAACAAAAA GGAGAGATCC ACTTTGTTAG TGAGGAGATT TTGTAAAAAT
    GACAGAGAAG TAAAGAAATC TGTGTATACT GGGACAAGGG CAATCATGAG AACTCTGCCT
    TCTGGTCACA TTGGGCTGGA AGCTTACAGT TACATCGATC AGAAGAGAAG TGGTCCCTTA
    TTACCCCGTG GGAGAGTTCT GGAACAGCTG CCAGTGGTGG CAATTAGGCA AGATGGGAGC
    CAGTGCCTGT CAGAAGCCCA GTGGTATCGG ATCTACAATG CAGTGAGAAG GGAAGAAGAA
    ATAGAAAATA CCATTGGATC TCTTCTCCAT TACTTTACCA AGCTCCCAGC CTCCAAGACA
    GCCCATGAAA GGATTAGTGT TGGTCCATGC CTAAAGCAGT GTGTCCGAGA CACCATATGT
    GAGTATCGTG CCACCCTCCA GAGGACGTCC ATATCTCAGT ACATCACGGG TTCGCTCCTA
    GAAGCAACCA CATCTTTAGG AGCGAGAAGT GGCCTACTCA GTACTTTTGG AGGATCCACT
    GGACGAATGA TGCTAAAAGA ACGCCAGCCA GGCACCTCCA TGGCCAATTC CAGTGCCCTC
    CCTTCAAGTT CCGCTGGAAT CAGCAAGGAG CTGATTGACC TGCAGCCTCT CATTCAGTTC
    CCAGAGGAAG TCGCCAGCAT CCTGATGGAG CAGGAACAGA ACATTTACCG AAGGGTCTTA
    CCAGTTGACT ATCTTTACTT CTTGACCCGG GACTTGGGCA CTCCAGAATG CCAGACACCC
    CTGCCCTGCC TCAAAGCATC CATCTCAGCT TCAATTCTTA GCTCCCAGAA CGGAGAGCAC
    AATGCCCTGG AAGATCTTGT GATGAGATTT AATGAGGTGA GCTCCTGGGT GACATGGCTG
    ATCCTCACGG CGGGCTCCAT GGAGGAGAAG CGAGAAGTCT TCTCCTATTT GGTGCATGTG
    GCCAAATGCT GCTGGAACAT GGGCAACTAC AATGCTGTCA TGGAGTTCTT GGCTGGTCTC
    AGGTCAAGAA AAGTTTTAAA AATGTGGCAG TTTATGGACC AATCTGACCT CGAGACCATG
    AGGAGCCTGA AGGACGCCAT GGCTCAGCAT GAATCTTCCT GCGAATATCG GAAGGTGATA
    ACGAGGGCTC TGCACATACC TGGCTGTAAA GTGGTTCCAT TCTGTGGAGT GTTTCTGAAG
    GAGCTCTGTG AAGTACTGGA CGGAGCCTCT GGCCTCATGA AGCTTTGCCC GAGGTACAAT
    TCCCAAGAAG AAACTCTAGA GTTTGTAGCA GATTACAGTG GACAAGATAA TTTCTTACAA
    CGAGTGGGGC ATAATGGCTT AAAGAATTCA GAGAAGGAGT CCACTGTCAA CAGCATCTTT
    CAAATCATTA GGAGCTGCAG CCGAAGTCTG GAGGCTGAGG AAGAGGACAG CCCCAGTGAA
    GGGAACAGCT CTAGGAAAAA TTCTTTAAGG GACAAAGCCC GATGGCAATT TATAATCGGA
    GATTTGTTGG ATTCCGAAAA TGACATCTTT GAGCAATCCA AAGAATGGGA CTCTCCCAGC
    TCAGAAGAGC CTCAGAAAGC CTTTGACCAT GGGACAGAGC TGATCCCTTG GTACGTGCTG
    TCCATCCGAG CAGATGTGCA CCAGTTCCTG CTACAGGGCG CCACCGTCCT CCGCTACGAC
    CAGGACACGC ACCTCTCTGC CCGCTGCTTC CTCCAGCTTC AGCCTGACAA TAGCACCTTG
    ACATGGATAA AGCCCACAGC TGCCTCCCCG GCCAGTGCTA GAGCAAAACT CGGTGTGCTG
    AGCAACACGT CTGAGCCTGG CAAGTTCCCG TCACCAGGCA GTGCCGGACT AAGCGGCCTG
    GCCGAGGGGG TCCTGGATCT GTTTTCAGCA AAGGCTGTGT ACATGGGACA CCCTAGCATT
    GATATCCACA CTGTGTGTGT CCAGAACAAA CTCGGTAGCA TGTTTCTCTC AGAGACTGGC
    GTGACCCTGC TCTACGGGCT CCAGACCACA GACAATAGAT TATTGCACTT TGTGGCACCC
    AAACACACTG CTAAGATGCT CTTCAGTGGA TTGCTAGAAC TCACGAGGGC CGTGAGGAAG
    ATGAGAAAGT TCCCTGACCA GAGGCAGCAG TGGCTGCGGA AACAGTATGT TAGCCTCTAT
    CAGGAGGACG GACGGTATGA GGGCCCAACT TTGGCTCATG CTGTGGAGTT GTTTGGTGGC
    CGGCGATGGA GTACACGCAA TCCCAGCCCT GGGACATCTG CAAAGAGTGC TGAGAAGCCC
    AGTGTGCAGA GAAACAACAC CTTGGGCATA AGCACCACCA AGAAAAAGAA GAAAATCCTT
    ATAAGGGGTG AGAGTGGAGA GGCAGCCGAC GATGAGATGG CCACCCGGAA GGCCAAAATG
    CACAGGGAGT GTCGCAGCCG GAGTGGTTCT GACCCACAAG ACATGAATGA GCAAGAAGAA
    TCTGAGGCAA ATGCCATCAT GAGCCCTCCT AACACTCTGC CTTCCAGAAG AGCCCACTCT
    TTGACTACAG CTGGGTCCCC TAATTTGTCT GCCGGGACAT CATCTCCCAT CAGTGCTTGG
    AGCAGCAGCA GCTGGCATGG AAGGATCAAA GGAGGAATGA AGGGTTTTCA GAGCTTCATG
    GTTTCAGACA GTAACATGAG TTTTGTTGAA TTTGTTGAGC TGTTCAAATC ATTCAGTGTG
    AGGAGCCGCA AGGACCTGAA GGATCTCTTT GACATCTACG CTGTGCCCTG CAATCGGGCC
    GGCTCTGAGT CGGCCCCGCT CTACACCAAC CTAACAATAG ACGAAAACAC CAGCGATCTT
    CAGCCTGACC TAGATTTATT GACCAGAAAC GTCTCGGATT TGGGGTTGTT CATTAAGAGT
    AAACAGCAGC TGTCAGACAA CCAGAGGCAG ATTTCTGATG CCATTGCTGC TGCAAGTATC
    GTGACGAATG GCACTGGGAT CGAGAGCACA TCTTTGGGCA TCTTTGGGGT TGGCATACTC
    CAGCTCAATG ACTTCCTAGT CAACTGCCAG GGAGAACACT GCACTTACGA TGAAATCCTC
    AGCATCATCC AGAAGTTCGA GCCTAGCATT AGTATGTGTC ATCAGGGCCT ACTGTCCTTT
    GAAGGATTTG CAAGGTTTCT GATGGATAAA GATAATTTTG CCTCCAAAAA TGATGAGTCA
    CAGGAGAACA TTAAAGATTT GCAGCTGCCT CTCTCCTACT ATTACATTGA GTCTTCACAC
    AATACCTACC TCACAGGCCA CCAGCTCAAA GGAGAGTCAT CGGTAGAACT CTATAGCCAG
    GTCCTCCTGC AAGGCTGTAG AAGTGTAGAG TTGGACTGCT GGGATGGAGA TGACGGGATG
    CCCATCATCT ATCACGGACA CACACTAACA ACCAAGATCC CCTTCAAGGA GGTGGTTGAA
    GCCATCGACC GCAGTGCCTT CATCAACTCT GACCTGCCCA TCATCATATC GATTGAGAAC
    CACTGCTCGT TGCCTCAGCA ACGAAAAATG GCAGAAATTT TCAAGACTGT GTTTGGAGAA
    AAACTGGTGG CTAAATTCCT GTTTGAGAGC GATTTCTCAG ATGATCCAAT GCTTCCCTCA
    CCTGACCAAC TTAGGAGGAA AGTGCTTCTT AAAAATAAGA AGCTAAAAGC CCACCAGACA
    CCAGTGGATA TCTTAAAGCA AAAGGCTCAT CAGTTAGCAT CCATGCAAGC TCAGGCTTAT
    AATGGGGGAA ATGTCAACCC TCCACCTGCT AATAACGAGG AAGAGGAAGA TGAAGAGGAC
    GAATATGATT ATGACTATGA ATCCCTGTCT GATGACAACA TTCTAGAAGA CAGACCTGAA
    AATAAATCAT GTAATGACAA GCTTCAGTTT GAGTATAACG AAGAAATCCC CAAGAGGATA
    AAGAAAGCAG ATAATTCTGC TTTCAACAAA GGAAAGGTTT ATGACATGGA ACTGGGAGAA
    GAATTTTATC TTCCTCAGAA TAAAAAGGAA AGCAGACAGA TTGCACCAGA GCTTTCTGAC
    CTTGTCATCT ACTGCCAAGC GGTAAAGTTT CCGGGGCTGT CAACTCTAAA TGCATCTGGC
    TCTAACAGAG GAAAAGAGAG GAAAAGCAGG AAGTCCATTT TTGGCAACAA TCCGGGCAGA
    ATGAGCCCTG GGGAGACAGC ATCGTTTAAC AAAGCATCTG GAAAAAGTTC CTGTGAAGGA
    ATGCGACAGG CGTGGGAGGA ATCTTCTTCC CCCCTCAACC CAACCACATC CCTCAGCGCT
    ATCATTAGAA CTCCCAAATG TTACCATATC TCATCGCTGA ATGAAAATGC CGCCAAACGT
    CTGTGTCGCA GGTATTCTCA GAAACTGATC CAGCACACTG CCTGTCAGCT GCTGAGGACT
    TACCCGGCTG CCACACGCAT CGACTCCTCC AACCCCAATC CTCTCTTGTT CTGGCTCCAT
    GGGATACAGC TCGTGGCACT CAACTACCAG ACAGATGATC TCCCTTTACA TTTAAATGCT
    GCCATGTTTG AGGCCAACGG TGGCTGTGGT TATGTTTTGA AACCCCCCGT TCTGTGGGAC
    AAGAACTGTC CCATGTACCA GAAGTTTTCA CCCTTGGAAA GAGACCTGGA CAACATGGAG
    CCTGCCGTCT ATTCCTTAAC TATTGTCTCT GGTCAAAACG TGTGCCCAAG TAGCAGCACG
    GGAAGCCCAT GTATCGAAGT GGACGTGCTG GGCATGCCCC TGGACAGTTG CCACTTCCGC
    ACAAAACCCA TCCACCGGAA CACTTTGAAC CCCATGTGGA ATGAACAGTT TCTCTTCCGG
    GTTCACTTTG AAGATCTCAT ATTCCTGCGT TTTGCAGTTG TGGAAAACAA TAGCTCAGCC
    ATAACCGCTC AGAGAATCAT CCCCCTCAAG GCTTTAAAAC GAGGATATCG ACATCTTCAG
    CTGCGAAACC TCCACAATGA AGTCTTGGAA ATCTCTAGTT TATTCATAAA CAGCAGAAGG
    ATGGAAGAAA ATTCCTCCAG CAGTGCCACG CCAGCCTCTT TGATGTTTAA TACAGAAGAA
    AGAAAATGTT TGCAGACTCA CAGAGTCACA GTGCATGGGG TCCCAGGTCC AGAGCCCTTT
    ACTGTTTTCT CCATAAGTGG AGGCACGAAG GCAAAGCAGC TTCTCCAACA GATTCTAACT
    ACTGAACAAG ACACCAAACC TATTGTCACA GACTATTTTT TGATGGAAGA AAAATATTTT
    ATATCTAAAG AAAAGAATGA ATGCAGGAAA CAACCATTTC AGAGAGTCAT CGGTCCAGAA
    GAAGAGATCA TGCAGATTTT AAGCAGCTGG TTTCCAGAGG AAGGATATGT TGGCAGGATT
    GTCTTAAAAA CCCAGCAGGA AAACCTAGAA GAGAGAAGCA TTGTTCAAGA TGAGAAAGAG
    GTGATCTTGA GCTCGGAGGA GGAGAGCTTC TTTGTCCAAG TGCATGACGT TTCTCCAGAG
    CAACCTCGGA CGGTCATCAA AGCGCCCCGT GTCAGCACCG CTCAGGATGT CATTCAACAG
    ACCTTATGCA AAGCCAAATA TTCCTATAGC ATCCTGAGTA ACCCCAACCC GAGTGACTAT
    GTGCTTCTGG AAGAGGTGGT GAAAGATGCT ACCAAGAAGT CCTCCACCCC AAAGTCCTCC
    CAGCGGGTCC TTTTGGACCA AGAGTGTGTG TTTCAAGCCC AGAGCAAGTG GAAAGGTGCT
    GGAAAATTCA TCCTCAGGCT AAAGGAGCAG GTGCAGGCAT CCCGAGAAGA TAAAAGAAAA
    GGCATTTCTT TCGCAAGTGA ATTCAAGAAG TTCACCAAAT CAACTAAACA GCCCCGAGGA
    CTCACATCTT CTCAGGTCTT GGCCTCAGAA AGTGTCCAAA ACAAGGAGGA GAAGCCCACG
    GGCAGCTTGT CCTCCAGTGA CACCACAGAC TCCCGACAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.