MICAL3 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	MICAL3
Entrez Gene ID	486761
Full Name	microtubule associated monooxygenase, calponin and LIM domain containing 3
General protein information	Preferred Names microtubule associated monooxygenase, calponin and LIM domain containing 3 Names protein-methionine sulfoxide oxidase MICAL3 F-actin-methionine sulfoxide oxidase MICAL3 microtubule associated monoxygenase, calponin and LIM domain containing 3
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014108387.2	XP_013963862.1	F-actin-methionine sulfoxide oxidase MICAL3 isoform X1
XM_014108388.2	XP_013963863.1	F-actin-methionine sulfoxide oxidase MICAL3 isoform X1
XM_022410882.1	XP_022266590.1	F-actin-methionine sulfoxide oxidase MICAL3 isoform X8
XM_022410886.1	XP_022266594.1	F-actin-methionine sulfoxide oxidase MICAL3 isoform X12
XM_022410887.1	XP_022266595.1	F-actin-methionine sulfoxide oxidase MICAL3 isoform X13
XM_022410894.1	XP_022266602.1	F-actin-methionine sulfoxide oxidase MICAL3 isoform X20
XM_022410898.1	XP_022266606.1	F-actin-methionine sulfoxide oxidase MICAL3 isoform X24
XM_014108383.1	XP_013963858.1	protein-methionine sulfoxide oxidase MICAL3 isoform X1
XM_014108389.1	XP_013963864.1	protein-methionine sulfoxide oxidase MICAL3 isoform X2
XM_014108384.1	XP_013963859.1	protein-methionine sulfoxide oxidase MICAL3 isoform X1
XM_014108390.1	XP_013963865.1	protein-methionine sulfoxide oxidase MICAL3 isoform X3
XM_014108383.2	XP_013963858.1	F-actin-methionine sulfoxide oxidase MICAL3 isoform X1
XM_022410874.1	XP_022266582.1	F-actin-methionine sulfoxide oxidase MICAL3 isoform X1
XM_022410877.1	XP_022266585.1	F-actin-methionine sulfoxide oxidase MICAL3 isoform X3
XM_022410881.1	XP_022266589.1	F-actin-methionine sulfoxide oxidase MICAL3 isoform X7
XM_022410884.1	XP_022266592.1	F-actin-methionine sulfoxide oxidase MICAL3 isoform X10
XM_022410885.1	XP_022266593.1	F-actin-methionine sulfoxide oxidase MICAL3 isoform X11
XM_022410889.1	XP_022266597.1	F-actin-methionine sulfoxide oxidase MICAL3 isoform X15
XM_022410879.1	XP_022266587.1	F-actin-methionine sulfoxide oxidase MICAL3 isoform X5
XM_022410880.1	XP_022266588.1	F-actin-methionine sulfoxide oxidase MICAL3 isoform X6
XM_022410890.1	XP_022266598.1	F-actin-methionine sulfoxide oxidase MICAL3 isoform X16
XM_022410891.1	XP_022266599.1	F-actin-methionine sulfoxide oxidase MICAL3 isoform X17
XM_022410893.1	XP_022266601.1	F-actin-methionine sulfoxide oxidase MICAL3 isoform X19
XM_014108386.1	XP_013963861.1	protein-methionine sulfoxide oxidase MICAL3 isoform X1
XM_014108386.2	XP_013963861.1	F-actin-methionine sulfoxide oxidase MICAL3 isoform X1
XM_022410875.1	XP_022266583.1	F-actin-methionine sulfoxide oxidase MICAL3 isoform X1
XM_022410883.1	XP_022266591.1	F-actin-methionine sulfoxide oxidase MICAL3 isoform X9
XM_022410892.1	XP_022266600.1	F-actin-methionine sulfoxide oxidase MICAL3 isoform X18
XM_022410896.1	XP_022266604.1	F-actin-methionine sulfoxide oxidase MICAL3 isoform X22
XM_014108385.1	XP_013963860.1	protein-methionine sulfoxide oxidase MICAL3 isoform X1
XM_014108387.1	XP_013963862.1	protein-methionine sulfoxide oxidase MICAL3 isoform X1
XM_014108388.1	XP_013963863.1	protein-methionine sulfoxide oxidase MICAL3 isoform X1
XM_022410876.1	XP_022266584.1	F-actin-methionine sulfoxide oxidase MICAL3 isoform X2
XM_022410878.1	XP_022266586.1	F-actin-methionine sulfoxide oxidase MICAL3 isoform X4
XM_022410888.1	XP_022266596.1	F-actin-methionine sulfoxide oxidase MICAL3 isoform X14
XM_022410895.1	XP_022266603.1	F-actin-methionine sulfoxide oxidase MICAL3 isoform X21
XM_022410897.1	XP_022266605.1	F-actin-methionine sulfoxide oxidase MICAL3 isoform X23

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Mical3	NP_001178014.1
Pan troglodytes (chimpanzee)	MICAL3	XP_001151830.2
	MICAL3	XP_001103660.2
Canis lupus familiaris (dog)	MICAL3	XP_005637453.1
Bos taurus (cattle)	MICAL3	XP_005207412.1
Danio rerio (zebrafish)	mical3a	XP_005159086.1
Homo sapiens (human)	MICAL3	NP_056056.2
Mus musculus (house mouse)	Mical3	NP_001257404.1
Gallus gallus (chicken)	MICAL3	XP_004938010.1
Xenopus tropicalis (tropical clawed frog)	mical3	XP_004912548.1

Gene Ontology

The following MICAL3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MICAL3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe27594	XM_014108390.1 Latest version!	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe27594	XM_022410895.1 Latest version!	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe282672	XM_022410876.1 Latest version!	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe282673	XM_022410877.1 Latest version!	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe282674	XM_022410878.1 Latest version!	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe282675	XM_022410879.1 Latest version!	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe282676	XM_022410880.1 Latest version!	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe282677	XM_022410881.1 Latest version!	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe282678	XM_022410882.1 Latest version!	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe282679	XM_022410883.1 Latest version!	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe282680	XM_022410884.1 Latest version!	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe282681	XM_022410885.1 Latest version!	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe282682	XM_022410886.1 Latest version!	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe282683	XM_022410887.1 Latest version!	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe282684	XM_022410888.1 Latest version!	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe282685	XM_022410889.1 Latest version!	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe282686	XM_022410890.1 Latest version!	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe282687	XM_022410891.1 Latest version!	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe282688	XM_022410892.1 Latest version!	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe282689	XM_022410893.1 Latest version!	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe282690	XM_022410896.1 Latest version!	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCe282691	XM_022410897.1 Latest version!	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCe282692	XM_022410898.1 Latest version!	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCe43613	XM_014108388.2 Latest version!	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe43613	XM_014108384.1 Latest version!	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe43613	XM_014108383.2 Latest version!	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe43613	XM_014108386.2 Latest version!	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe43613	XM_014108387.2 Latest version!	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe43613	XM_022410874.1 Latest version!	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe43613	XM_022410875.1 Latest version!	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe43613	XM_014108385.1 Latest version!	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe43614	XM_014108389.1 Latest version!	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe43614	XM_022410894.1 Latest version!	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
View Old Accession Versions >>
OCe43613	XM_014108383.1	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe43613	XM_014108386.1	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe43613	XM_014108387.1	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe43613	XM_014108388.1	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MICAL3 ( XM_014108390.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963865.1 Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X8, mRNA.

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CloneID	OCe27594
Clone ID Related Accession (Same CDS sequence)	XM_014108390.1 , XM_022410895.1
Accession Version	XM_014108390.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6051bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GGGCTCGATA AAGAAGGAGT TCCCACAGAA CTTAGGAGGC 
AGCGACACGT GCTATTTCTG CCGGAAGCGG GTCTACGTGA TGGAAAGGCT GAGTGCTGAG 
GGCAAGTTCT TCCATCGGAG CTGCTTCAAG TGCGAGTACT GCGCCACCAC CCTGCGCCTC 
TCGGCCTATG CCTATGACAT TGAGGACGGT AAATTCTACT GCAAACCACA CTACTGTTAT 
CGACTCTCTG GCTCTGCACA GAGGAAGAGA CCAGCAGTGG CTCCTCTCTC TGGAAAGGAG 
GCCAGAGAAC CCCTGCAGGA TGGCCCCACT GTGGACACAA ATGGACGGGC CAGCGCTGTC 
ACCAGCCCTG CTGAGAGAAC TCCAGGTTCA AGCGTGAATG GTGTGGAAGA GCCTAGCATT 
GCCAAGAGGC TCAGGGGCAC TCCCGAGCGG ATTGAGCTGG AGAACTACCG CCTGTCCGTG 
AAGCAGGCCG AGGAGCTGGA GGAGGTGCCC GAGGAGACAC AGGCCGAGCA CAACCTGAGC 
AGCGTGCTGG ACACGGGGGC AGAGGAGGAC GTGGCCAGCA GCAGTTCTGA GTCAGAGATG 
GAGGAAGAGG AGGAAGAAGA GCCTCCTCTG CCCACTTCGG ACCTGGGCGG TGTTCCTTGG 
AAGGAGGCCG TGAGGATCCA TGCTCTTCTG AAAGGAAAGA GTGAAGAGGA GCTAGAGGCC 
TCAAAGAGCT TTGGGCCTGG GCATGAAGAA GACGAGGAGG AGGAGGAGTA TGAAGAGGAG 
GAGGAGGAGG AGGAGGAAGA ATATGAAGAG GAAGAGGAAG AGGAAGAGTC TAGTGAAGTT 
GGGAGCCAGA GGCTACAGCA GATCGTAAAT CCGGCCGACC CCTTGGAGAT CCAGGCTGAT 
GTGCACTGGA CACATATCCG AGAGAGAGAG GAGGAGGAGC GAATGGTCCC AACCTCTGAG 
TCCTCCACTT CTAGAGTCCC ATTTGATGAG AATGACCTGG AGGAAGACGT GGACTCGGAG 
CCAGCAAAGA TAGAAGGGGA GGCCACAGAG AATGGGGACA CAGGGGACAC TGGTGCTGAG 
CTGGACGATG ATCAGCGCTG GTCTGACGGG ATCCCATCGG ATGCCGAGAC GGATCTCCGC 
GTGCAGCACC AGGAGGCGGT AGAGGCAGAC CTGGAGCTGA GGGTCTCAGA GAATGAGGAG 
GAGCCACCTG AGGTCACACC AGGGCATCCG GAGAGAAGTC TCACCCGGGT CCCCAGCCCT 
GCCCTGTCCC CTGAGGAGCA AGCTGGTCTG CGGTCCCCAG GCCACAGCCC TAAGGCAGAG 
GGGGCCCAAA TCCCACTTGT CTCTGTTGTC ACCAAGGCAA AATTGCCTGA AGAGAACCTT 
TTCCCCAAGC CTTTGCTCCC CAAAGTGAAG CCCAAGGTGG AAGTGCCCTG CAGTCAGAGA 
GCTGCATGCT CCCCCATCCG TTCTCAGCCA GTGGCTCTGC CAGAAGCCAG GACACCTACC 
TCCCCAGTGA GCTCGCAGTG CCTGTCGCCT TTGGCCACCT CCACCCCCAC CAGCACCCAG 
CTCCCCATTT GCTCCCAGCC TCAGCCTTCC TCTGAGACCA CGGCCCCATC CCCCACCAAG 
TCCCCCATAC GGCTCCAGCC TGTTTCAGCC AAAACATCTA TCCCAGTGGT TCCCCTCCCC 
TTGAAGAACC AAGGGGACAC CAAGGACAGG CTGGGCAGCC CTCTCGCTGT GGATGAGGCC 
CTGAAGCGGA ATGACCTGGT GGCAGAGTTC TGGATGAAGA GCGCCGAGAT CCGCCGCAGC 
CTCGGGCTCA CACCAGTGGA TCGGAGCAAG GTGTATGAGC CCAGCTTCCC TTCGCCTGCC 
TTTAAACCAG TGTCCCTAAA ATCCTATGCA GTGGAGAAGT CTCCCGAGGG GGAGGGACTC 
CAGCTTCTGA AGCCTCCACC TGTTCCTAAG AGACTAGGCC TGCCGAAGTC AGACGGTGAC 
CAGCCCTTCC TGCCAACCCC CAAGTCCCCG TCGGACAGAG AGCTGAAAAG CTCCCAGGAG 
GAGAGGAGAG ATCTGTCGAG CAGCTCCGGG CTGGGCCTTC ACGGGAGCTC CTCCAACATG 
AAGACTTTGG GCAGCCAGAG CTTCAACACA TCAGACTCCA CCATGCTCAC TCCCCCTTCA 
AGCCCACCGC CACCACCACC CCAAGACGAG GAACCCGCAA CTCTTCGGAG GAAGCCATAT 
CAGACTCATG AGCATAAGGA CGCTGAGCCC AAGGCCTCGG TGATTCCACC ACCACCACCT 
GCGACGTTCA TGCGGGCCCC CCGGGAGCCC ACCCAGCCCC TCCAAGAGGA GATGCGGAAG 
TCGTTTGTGG AGAGCGTGGA TGAGATCCCA TTTGCCGATG ATGTGGAGGA CACCTATGAT 
GACAAGACCG AGGACTCGAG TCTCCAGGAG AAGTTCTTCA CACCCCCCTC CTACTGGCCC 
CGCACGGAGA GGCCCCTCCA CCCACCCCTA GCCAAGGAGA ATGGTAGGTT GCCTACTCTG 
GAGAGTGGGG TCCAGCCACA GAAGAGGGGC CTGCCCTTAG TCTCTCCAGA AGCCAAGGAG 
CTGGCTGAGG AGCGCATGCG AGCCAGGGAG AAGTCTGTGA AGAGCCAGGC GTTGAGAGAC 
GCTATGGCCA AGCAGCTGAG TAAGATGAAG GAAATGGAGA TGGCGGCTGG GACCTCCAGT 
CCCCCAGGAG GCACCTCCCA CAAAGCCTCC TCTGTTCCCT CCAAGGGCAA AGACCTCAGC 
CCCGAGTCCC CTAGACGCCC AGTTCTCAAA GGCCCCAGGG AGCCTGCCCT GAAGCACGAA 
GCCACTAGTG AGGAGGTCCT CTCCCCACCA TCGGACTCAG GGGGCCCAGA TGGTTCGGTC 
ACCTCATCCG AGGGCTCCAG TGGGAAGAGC AAGAAGAGAT CGTCACTCTT CTCCCCCCGC 
AGAAACAAGA AAGAGAAGAA GTCCAAAGGT GAGGGCCGGC CCCCGGAGAA GCCCAGCCCA 
AGCCTTCTGG AGGAAGCTGC TGCCAAGCCC AAGTCCCTGT GGAAGTCGGT CTTTTCCGGG 
TACAAGAAGG ACAAAAAGAA GAAGGGTGAT GACAAGTCCT GTTCCAGCAC CCCTTCCAGC 
GGTGCCACTG TGGACTCTGG CAAGCACAAG ATGTCCCCTG TTGTGAGAGC AGAGCTGCAC 
CTGCGGCGCC AGTTGAGCTT CTCCGAGGAC TCAGACCTGT CCAGTGATGA CATCCTTGAG 
AGGTCCTCCC AGAAGTCCAG GCGAGAGCCG AGAACCTACA CAGAGGAGGA ACTGAATGCC 
AAGCTGACCC GGCGTGTGCA GAAGGCAGCT CGTAGACAGG CCAAGCAAGA GGAGCTGAAG 
CGGCTGCACC GAGCACAGAT CATCCAGAGG CAGCTGGAGC AGGTGGAGGA GAAGCAGAGA 
CAGCTGGAGG AAAGAGGGGT GGCCGTGGAG AAGGCGCTCC GTGGTGAAGC AGGCATGGGC 
AAGAAGGATG ACCCCAAGCT GATGCAGGAG TGGTTCAAGT TGGTGCAAGA GAAGAATGCC 
ATGGTGCGCT ATGAGTCGGA GCTGATGATT TTTGCCCGGG AGCTGGAACT GGAGGACCGG 
CAGAGCCGAC TGCAGCAGGA GCTCCGGGAG CGGATGGCGG TGGAAGATCA CCTGAAGACT 
GAGGAGGAGC TGTCTGAGGA GAAGAAGATC TTGAACGAGA TGCTGGAGGT GGTGGAGCAG 
AGAGATGCCC TCGTGGCCCT GCTTGAGGAG CAGCGACTCC GGGAGAAGGA GGAGGACAAA 
GACCTGGAGG CTGTCATGCT GTCCAAGGGC TTCAGCCTGA ACTGGTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963865.1
CDS	1090..7140
Translation	MEDSKNETVNHAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDYRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKACTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFQGLVQPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELKEATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLNYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREQNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNININFLRPSQVRHLYNTGETRDIHLEMENLVNSRTTPKLTRNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGKEMASVGEPDKLSMVMYLTQFYEMFKDSLPSSDALDLNAEEKAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEEAPRGYRGGRPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSTGMRRQGSIKKEFPQNLGGSDTCYFCRKRVYVMERLSAEGKFFHRSCFKCEYCATTLRLSAYAYDIEDGKFYCKPHYCYRLSGSAQRKRPAVAPLSGKEAREPLQDGPTVDTNGRASAVTSPAERTPGSSVNGVEEPSIAKRLRGTPERIELENYRLSVKQAEELEEVPEETQAEHNLSSVLDTGAEEDVASSSSESEMEEEEEEEPPLPTSDLGGVPWKEAVRIHALLKGKSEEELEASKSFGPGHEEDEEEEEYEEEEEEEEEEYEEEEEEEESSEVGSQRLQQIVNPADPLEIQADVHWTHIREREEEERMVPTSESSTSRVPFDENDLEEDVDSEPAKIEGEATENGDTGDTGAELDDDQRWSDGIPSDAETDLRVQHQEAVEADLELRVSENEEEPPEVTPGHPERSLTRVPSPALSPEEQAGLRSPGHSPKAEGAQIPLVSVVTKAKLPEENLFPKPLLPKVKPKVEVPCSQRAACSPIRSQPVALPEARTPTSPVSSQCLSPLATSTPTSTQLPICSQPQPSSETTAPSPTKSPIRLQPVSAKTSIPVVPLPLKNQGDTKDRLGSPLAVDEALKRNDLVAEFWMKSAEIRRSLGLTPVDRSKVYEPSFPSPAFKPVSLKSYAVEKSPEGEGLQLLKPPPVPKRLGLPKSDGDQPFLPTPKSPSDRELKSSQEERRDLSSSSGLGLHGSSSNMKTLGSQSFNTSDSTMLTPPSSPPPPPPQDEEPATLRRKPYQTHEHKDAEPKASVIPPPPPATFMRAPREPTQPLQEEMRKSFVESVDEIPFADDVEDTYDDKTEDSSLQEKFFTPPSYWPRTERPLHPPLAKENGRLPTLESGVQPQKRGLPLVSPEAKELAEERMRAREKSVKSQALRDAMAKQLSKMKEMEMAAGTSSPPGGTSHKASSVPSKGKDLSPESPRRPVLKGPREPALKHEATSEEVLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRNKKEKKSKGEGRPPEKPSPSLLEEAAAKPKSLWKSVFSGYKKDKKKKGDDKSCSSTPSSGATVDSGKHKMSPVVRAELHLRRQLSFSEDSDLSSDDILERSSQKSRREPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELSEEKKILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAVMLSKGFSLNWS

Target ORF information:

RefSeq Version	XM_014108390.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108390.1

ORF Insert Sequence:

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GGGCTCGATA AAGAAGGAGT TCCCACAGAA CTTAGGAGGC 
AGCGACACGT GCTATTTCTG CCGGAAGCGG GTCTACGTGA TGGAAAGGCT GAGTGCTGAG 
GGCAAGTTCT TCCATCGGAG CTGCTTCAAG TGCGAGTACT GCGCCACCAC CCTGCGCCTC 
TCGGCCTATG CCTATGACAT TGAGGACGGT AAATTCTACT GCAAACCACA CTACTGTTAT 
CGACTCTCTG GCTCTGCACA GAGGAAGAGA CCAGCAGTGG CTCCTCTCTC TGGAAAGGAG 
GCCAGAGAAC CCCTGCAGGA TGGCCCCACT GTGGACACAA ATGGACGGGC CAGCGCTGTC 
ACCAGCCCTG CTGAGAGAAC TCCAGGTTCA AGCGTGAATG GTGTGGAAGA GCCTAGCATT 
GCCAAGAGGC TCAGGGGCAC TCCCGAGCGG ATTGAGCTGG AGAACTACCG CCTGTCCGTG 
AAGCAGGCCG AGGAGCTGGA GGAGGTGCCC GAGGAGACAC AGGCCGAGCA CAACCTGAGC 
AGCGTGCTGG ACACGGGGGC AGAGGAGGAC GTGGCCAGCA GCAGTTCTGA GTCAGAGATG 
GAGGAAGAGG AGGAAGAAGA GCCTCCTCTG CCCACTTCGG ACCTGGGCGG TGTTCCTTGG 
AAGGAGGCCG TGAGGATCCA TGCTCTTCTG AAAGGAAAGA GTGAAGAGGA GCTAGAGGCC 
TCAAAGAGCT TTGGGCCTGG GCATGAAGAA GACGAGGAGG AGGAGGAGTA TGAAGAGGAG 
GAGGAGGAGG AGGAGGAAGA ATATGAAGAG GAAGAGGAAG AGGAAGAGTC TAGTGAAGTT 
GGGAGCCAGA GGCTACAGCA GATCGTAAAT CCGGCCGACC CCTTGGAGAT CCAGGCTGAT 
GTGCACTGGA CACATATCCG AGAGAGAGAG GAGGAGGAGC GAATGGTCCC AACCTCTGAG 
TCCTCCACTT CTAGAGTCCC ATTTGATGAG AATGACCTGG AGGAAGACGT GGACTCGGAG 
CCAGCAAAGA TAGAAGGGGA GGCCACAGAG AATGGGGACA CAGGGGACAC TGGTGCTGAG 
CTGGACGATG ATCAGCGCTG GTCTGACGGG ATCCCATCGG ATGCCGAGAC GGATCTCCGC 
GTGCAGCACC AGGAGGCGGT AGAGGCAGAC CTGGAGCTGA GGGTCTCAGA GAATGAGGAG 
GAGCCACCTG AGGTCACACC AGGGCATCCG GAGAGAAGTC TCACCCGGGT CCCCAGCCCT 
GCCCTGTCCC CTGAGGAGCA AGCTGGTCTG CGGTCCCCAG GCCACAGCCC TAAGGCAGAG 
GGGGCCCAAA TCCCACTTGT CTCTGTTGTC ACCAAGGCAA AATTGCCTGA AGAGAACCTT 
TTCCCCAAGC CTTTGCTCCC CAAAGTGAAG CCCAAGGTGG AAGTGCCCTG CAGTCAGAGA 
GCTGCATGCT CCCCCATCCG TTCTCAGCCA GTGGCTCTGC CAGAAGCCAG GACACCTACC 
TCCCCAGTGA GCTCGCAGTG CCTGTCGCCT TTGGCCACCT CCACCCCCAC CAGCACCCAG 
CTCCCCATTT GCTCCCAGCC TCAGCCTTCC TCTGAGACCA CGGCCCCATC CCCCACCAAG 
TCCCCCATAC GGCTCCAGCC TGTTTCAGCC AAAACATCTA TCCCAGTGGT TCCCCTCCCC 
TTGAAGAACC AAGGGGACAC CAAGGACAGG CTGGGCAGCC CTCTCGCTGT GGATGAGGCC 
CTGAAGCGGA ATGACCTGGT GGCAGAGTTC TGGATGAAGA GCGCCGAGAT CCGCCGCAGC 
CTCGGGCTCA CACCAGTGGA TCGGAGCAAG GTGTATGAGC CCAGCTTCCC TTCGCCTGCC 
TTTAAACCAG TGTCCCTAAA ATCCTATGCA GTGGAGAAGT CTCCCGAGGG GGAGGGACTC 
CAGCTTCTGA AGCCTCCACC TGTTCCTAAG AGACTAGGCC TGCCGAAGTC AGACGGTGAC 
CAGCCCTTCC TGCCAACCCC CAAGTCCCCG TCGGACAGAG AGCTGAAAAG CTCCCAGGAG 
GAGAGGAGAG ATCTGTCGAG CAGCTCCGGG CTGGGCCTTC ACGGGAGCTC CTCCAACATG 
AAGACTTTGG GCAGCCAGAG CTTCAACACA TCAGACTCCA CCATGCTCAC TCCCCCTTCA 
AGCCCACCGC CACCACCACC CCAAGACGAG GAACCCGCAA CTCTTCGGAG GAAGCCATAT 
CAGACTCATG AGCATAAGGA CGCTGAGCCC AAGGCCTCGG TGATTCCACC ACCACCACCT 
GCGACGTTCA TGCGGGCCCC CCGGGAGCCC ACCCAGCCCC TCCAAGAGGA GATGCGGAAG 
TCGTTTGTGG AGAGCGTGGA TGAGATCCCA TTTGCCGATG ATGTGGAGGA CACCTATGAT 
GACAAGACCG AGGACTCGAG TCTCCAGGAG AAGTTCTTCA CACCCCCCTC CTACTGGCCC 
CGCACGGAGA GGCCCCTCCA CCCACCCCTA GCCAAGGAGA ATGGTAGGTT GCCTACTCTG 
GAGAGTGGGG TCCAGCCACA GAAGAGGGGC CTGCCCTTAG TCTCTCCAGA AGCCAAGGAG 
CTGGCTGAGG AGCGCATGCG AGCCAGGGAG AAGTCTGTGA AGAGCCAGGC GTTGAGAGAC 
GCTATGGCCA AGCAGCTGAG TAAGATGAAG GAAATGGAGA TGGCGGCTGG GACCTCCAGT 
CCCCCAGGAG GCACCTCCCA CAAAGCCTCC TCTGTTCCCT CCAAGGGCAA AGACCTCAGC 
CCCGAGTCCC CTAGACGCCC AGTTCTCAAA GGCCCCAGGG AGCCTGCCCT GAAGCACGAA 
GCCACTAGTG AGGAGGTCCT CTCCCCACCA TCGGACTCAG GGGGCCCAGA TGGTTCGGTC 
ACCTCATCCG AGGGCTCCAG TGGGAAGAGC AAGAAGAGAT CGTCACTCTT CTCCCCCCGC 
AGAAACAAGA AAGAGAAGAA GTCCAAAGGT GAGGGCCGGC CCCCGGAGAA GCCCAGCCCA 
AGCCTTCTGG AGGAAGCTGC TGCCAAGCCC AAGTCCCTGT GGAAGTCGGT CTTTTCCGGG 
TACAAGAAGG ACAAAAAGAA GAAGGGTGAT GACAAGTCCT GTTCCAGCAC CCCTTCCAGC 
GGTGCCACTG TGGACTCTGG CAAGCACAAG ATGTCCCCTG TTGTGAGAGC AGAGCTGCAC 
CTGCGGCGCC AGTTGAGCTT CTCCGAGGAC TCAGACCTGT CCAGTGATGA CATCCTTGAG 
AGGTCCTCCC AGAAGTCCAG GCGAGAGCCG AGAACCTACA CAGAGGAGGA ACTGAATGCC 
AAGCTGACCC GGCGTGTGCA GAAGGCAGCT CGTAGACAGG CCAAGCAAGA GGAGCTGAAG 
CGGCTGCACC GAGCACAGAT CATCCAGAGG CAGCTGGAGC AGGTGGAGGA GAAGCAGAGA 
CAGCTGGAGG AAAGAGGGGT GGCCGTGGAG AAGGCGCTCC GTGGTGAAGC AGGCATGGGC 
AAGAAGGATG ACCCCAAGCT GATGCAGGAG TGGTTCAAGT TGGTGCAAGA GAAGAATGCC 
ATGGTGCGCT ATGAGTCGGA GCTGATGATT TTTGCCCGGG AGCTGGAACT GGAGGACCGG 
CAGAGCCGAC TGCAGCAGGA GCTCCGGGAG CGGATGGCGG TGGAAGATCA CCTGAAGACT 
GAGGAGGAGC TGTCTGAGGA GAAGAAGATC TTGAACGAGA TGCTGGAGGT GGTGGAGCAG 
AGAGATGCCC TCGTGGCCCT GCTTGAGGAG CAGCGACTCC GGGAGAAGGA GGAGGACAAA 
GACCTGGAGG CTGTCATGCT GTCCAAGGGC TTCAGCCTGA ACTGGTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_022410895.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022266603.1 Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X26, mRNA.

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CloneID	OCe27594
Clone ID Related Accession (Same CDS sequence)	XM_014108390.1 , XM_022410895.1
Accession Version	XM_022410895.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6051bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	F-actin-methionine sulfoxide oxidase MICAL3 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GGGCTCGATA AAGAAGGAGT TCCCACAGAA CTTAGGAGGC 
AGCGACACGT GCTATTTCTG CCGGAAGCGG GTCTACGTGA TGGAAAGGCT GAGTGCTGAG 
GGCAAGTTCT TCCATCGGAG CTGCTTCAAG TGCGAGTACT GCGCCACCAC CCTGCGCCTC 
TCGGCCTATG CCTATGACAT TGAGGACGGT AAATTCTACT GCAAACCACA CTACTGTTAT 
CGACTCTCTG GCTCTGCACA GAGGAAGAGA CCAGCAGTGG CTCCTCTCTC TGGAAAGGAG 
GCCAGAGAAC CCCTGCAGGA TGGCCCCACT GTGGACACAA ATGGACGGGC CAGCGCTGTC 
ACCAGCCCTG CTGAGAGAAC TCCAGGTTCA AGCGTGAATG GTGTGGAAGA GCCTAGCATT 
GCCAAGAGGC TCAGGGGCAC TCCCGAGCGG ATTGAGCTGG AGAACTACCG CCTGTCCGTG 
AAGCAGGCCG AGGAGCTGGA GGAGGTGCCC GAGGAGACAC AGGCCGAGCA CAACCTGAGC 
AGCGTGCTGG ACACGGGGGC AGAGGAGGAC GTGGCCAGCA GCAGTTCTGA GTCAGAGATG 
GAGGAAGAGG AGGAAGAAGA GCCTCCTCTG CCCACTTCGG ACCTGGGCGG TGTTCCTTGG 
AAGGAGGCCG TGAGGATCCA TGCTCTTCTG AAAGGAAAGA GTGAAGAGGA GCTAGAGGCC 
TCAAAGAGCT TTGGGCCTGG GCATGAAGAA GACGAGGAGG AGGAGGAGTA TGAAGAGGAG 
GAGGAGGAGG AGGAGGAAGA ATATGAAGAG GAAGAGGAAG AGGAAGAGTC TAGTGAAGTT 
GGGAGCCAGA GGCTACAGCA GATCGTAAAT CCGGCCGACC CCTTGGAGAT CCAGGCTGAT 
GTGCACTGGA CACATATCCG AGAGAGAGAG GAGGAGGAGC GAATGGTCCC AACCTCTGAG 
TCCTCCACTT CTAGAGTCCC ATTTGATGAG AATGACCTGG AGGAAGACGT GGACTCGGAG 
CCAGCAAAGA TAGAAGGGGA GGCCACAGAG AATGGGGACA CAGGGGACAC TGGTGCTGAG 
CTGGACGATG ATCAGCGCTG GTCTGACGGG ATCCCATCGG ATGCCGAGAC GGATCTCCGC 
GTGCAGCACC AGGAGGCGGT AGAGGCAGAC CTGGAGCTGA GGGTCTCAGA GAATGAGGAG 
GAGCCACCTG AGGTCACACC AGGGCATCCG GAGAGAAGTC TCACCCGGGT CCCCAGCCCT 
GCCCTGTCCC CTGAGGAGCA AGCTGGTCTG CGGTCCCCAG GCCACAGCCC TAAGGCAGAG 
GGGGCCCAAA TCCCACTTGT CTCTGTTGTC ACCAAGGCAA AATTGCCTGA AGAGAACCTT 
TTCCCCAAGC CTTTGCTCCC CAAAGTGAAG CCCAAGGTGG AAGTGCCCTG CAGTCAGAGA 
GCTGCATGCT CCCCCATCCG TTCTCAGCCA GTGGCTCTGC CAGAAGCCAG GACACCTACC 
TCCCCAGTGA GCTCGCAGTG CCTGTCGCCT TTGGCCACCT CCACCCCCAC CAGCACCCAG 
CTCCCCATTT GCTCCCAGCC TCAGCCTTCC TCTGAGACCA CGGCCCCATC CCCCACCAAG 
TCCCCCATAC GGCTCCAGCC TGTTTCAGCC AAAACATCTA TCCCAGTGGT TCCCCTCCCC 
TTGAAGAACC AAGGGGACAC CAAGGACAGG CTGGGCAGCC CTCTCGCTGT GGATGAGGCC 
CTGAAGCGGA ATGACCTGGT GGCAGAGTTC TGGATGAAGA GCGCCGAGAT CCGCCGCAGC 
CTCGGGCTCA CACCAGTGGA TCGGAGCAAG GTGTATGAGC CCAGCTTCCC TTCGCCTGCC 
TTTAAACCAG TGTCCCTAAA ATCCTATGCA GTGGAGAAGT CTCCCGAGGG GGAGGGACTC 
CAGCTTCTGA AGCCTCCACC TGTTCCTAAG AGACTAGGCC TGCCGAAGTC AGACGGTGAC 
CAGCCCTTCC TGCCAACCCC CAAGTCCCCG TCGGACAGAG AGCTGAAAAG CTCCCAGGAG 
GAGAGGAGAG ATCTGTCGAG CAGCTCCGGG CTGGGCCTTC ACGGGAGCTC CTCCAACATG 
AAGACTTTGG GCAGCCAGAG CTTCAACACA TCAGACTCCA CCATGCTCAC TCCCCCTTCA 
AGCCCACCGC CACCACCACC CCAAGACGAG GAACCCGCAA CTCTTCGGAG GAAGCCATAT 
CAGACTCATG AGCATAAGGA CGCTGAGCCC AAGGCCTCGG TGATTCCACC ACCACCACCT 
GCGACGTTCA TGCGGGCCCC CCGGGAGCCC ACCCAGCCCC TCCAAGAGGA GATGCGGAAG 
TCGTTTGTGG AGAGCGTGGA TGAGATCCCA TTTGCCGATG ATGTGGAGGA CACCTATGAT 
GACAAGACCG AGGACTCGAG TCTCCAGGAG AAGTTCTTCA CACCCCCCTC CTACTGGCCC 
CGCACGGAGA GGCCCCTCCA CCCACCCCTA GCCAAGGAGA ATGGTAGGTT GCCTACTCTG 
GAGAGTGGGG TCCAGCCACA GAAGAGGGGC CTGCCCTTAG TCTCTCCAGA AGCCAAGGAG 
CTGGCTGAGG AGCGCATGCG AGCCAGGGAG AAGTCTGTGA AGAGCCAGGC GTTGAGAGAC 
GCTATGGCCA AGCAGCTGAG TAAGATGAAG GAAATGGAGA TGGCGGCTGG GACCTCCAGT 
CCCCCAGGAG GCACCTCCCA CAAAGCCTCC TCTGTTCCCT CCAAGGGCAA AGACCTCAGC 
CCCGAGTCCC CTAGACGCCC AGTTCTCAAA GGCCCCAGGG AGCCTGCCCT GAAGCACGAA 
GCCACTAGTG AGGAGGTCCT CTCCCCACCA TCGGACTCAG GGGGCCCAGA TGGTTCGGTC 
ACCTCATCCG AGGGCTCCAG TGGGAAGAGC AAGAAGAGAT CGTCACTCTT CTCCCCCCGC 
AGAAACAAGA AAGAGAAGAA GTCCAAAGGT GAGGGCCGGC CCCCGGAGAA GCCCAGCCCA 
AGCCTTCTGG AGGAAGCTGC TGCCAAGCCC AAGTCCCTGT GGAAGTCGGT CTTTTCCGGG 
TACAAGAAGG ACAAAAAGAA GAAGGGTGAT GACAAGTCCT GTTCCAGCAC CCCTTCCAGC 
GGTGCCACTG TGGACTCTGG CAAGCACAAG ATGTCCCCTG TTGTGAGAGC AGAGCTGCAC 
CTGCGGCGCC AGTTGAGCTT CTCCGAGGAC TCAGACCTGT CCAGTGATGA CATCCTTGAG 
AGGTCCTCCC AGAAGTCCAG GCGAGAGCCG AGAACCTACA CAGAGGAGGA ACTGAATGCC 
AAGCTGACCC GGCGTGTGCA GAAGGCAGCT CGTAGACAGG CCAAGCAAGA GGAGCTGAAG 
CGGCTGCACC GAGCACAGAT CATCCAGAGG CAGCTGGAGC AGGTGGAGGA GAAGCAGAGA 
CAGCTGGAGG AAAGAGGGGT GGCCGTGGAG AAGGCGCTCC GTGGTGAAGC AGGCATGGGC 
AAGAAGGATG ACCCCAAGCT GATGCAGGAG TGGTTCAAGT TGGTGCAAGA GAAGAATGCC 
ATGGTGCGCT ATGAGTCGGA GCTGATGATT TTTGCCCGGG AGCTGGAACT GGAGGACCGG 
CAGAGCCGAC TGCAGCAGGA GCTCCGGGAG CGGATGGCGG TGGAAGATCA CCTGAAGACT 
GAGGAGGAGC TGTCTGAGGA GAAGAAGATC TTGAACGAGA TGCTGGAGGT GGTGGAGCAG 
AGAGATGCCC TCGTGGCCCT GCTTGAGGAG CAGCGACTCC GGGAGAAGGA GGAGGACAAA 
GACCTGGAGG CTGTCATGCT GTCCAAGGGC TTCAGCCTGA ACTGGTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022266603.1
CDS	257..6307
Translation	MEDSKNETVNHAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDYRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKACTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFQGLVQPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELKEATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLNYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREQNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNININFLRPSQVRHLYNTGETRDIHLEMENLVNSRTTPKLTRNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGKEMASVGEPDKLSMVMYLTQFYEMFKDSLPSSDALDLNAEEKAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEEAPRGYRGGRPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSTGMRRQGSIKKEFPQNLGGSDTCYFCRKRVYVMERLSAEGKFFHRSCFKCEYCATTLRLSAYAYDIEDGKFYCKPHYCYRLSGSAQRKRPAVAPLSGKEAREPLQDGPTVDTNGRASAVTSPAERTPGSSVNGVEEPSIAKRLRGTPERIELENYRLSVKQAEELEEVPEETQAEHNLSSVLDTGAEEDVASSSSESEMEEEEEEEPPLPTSDLGGVPWKEAVRIHALLKGKSEEELEASKSFGPGHEEDEEEEEYEEEEEEEEEEYEEEEEEEESSEVGSQRLQQIVNPADPLEIQADVHWTHIREREEEERMVPTSESSTSRVPFDENDLEEDVDSEPAKIEGEATENGDTGDTGAELDDDQRWSDGIPSDAETDLRVQHQEAVEADLELRVSENEEEPPEVTPGHPERSLTRVPSPALSPEEQAGLRSPGHSPKAEGAQIPLVSVVTKAKLPEENLFPKPLLPKVKPKVEVPCSQRAACSPIRSQPVALPEARTPTSPVSSQCLSPLATSTPTSTQLPICSQPQPSSETTAPSPTKSPIRLQPVSAKTSIPVVPLPLKNQGDTKDRLGSPLAVDEALKRNDLVAEFWMKSAEIRRSLGLTPVDRSKVYEPSFPSPAFKPVSLKSYAVEKSPEGEGLQLLKPPPVPKRLGLPKSDGDQPFLPTPKSPSDRELKSSQEERRDLSSSSGLGLHGSSSNMKTLGSQSFNTSDSTMLTPPSSPPPPPPQDEEPATLRRKPYQTHEHKDAEPKASVIPPPPPATFMRAPREPTQPLQEEMRKSFVESVDEIPFADDVEDTYDDKTEDSSLQEKFFTPPSYWPRTERPLHPPLAKENGRLPTLESGVQPQKRGLPLVSPEAKELAEERMRAREKSVKSQALRDAMAKQLSKMKEMEMAAGTSSPPGGTSHKASSVPSKGKDLSPESPRRPVLKGPREPALKHEATSEEVLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRNKKEKKSKGEGRPPEKPSPSLLEEAAAKPKSLWKSVFSGYKKDKKKKGDDKSCSSTPSSGATVDSGKHKMSPVVRAELHLRRQLSFSEDSDLSSDDILERSSQKSRREPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELSEEKKILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAVMLSKGFSLNWS

Target ORF information:

RefSeq Version	XM_022410895.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022410895.1

ORF Insert Sequence:

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GGGCTCGATA AAGAAGGAGT TCCCACAGAA CTTAGGAGGC 
AGCGACACGT GCTATTTCTG CCGGAAGCGG GTCTACGTGA TGGAAAGGCT GAGTGCTGAG 
GGCAAGTTCT TCCATCGGAG CTGCTTCAAG TGCGAGTACT GCGCCACCAC CCTGCGCCTC 
TCGGCCTATG CCTATGACAT TGAGGACGGT AAATTCTACT GCAAACCACA CTACTGTTAT 
CGACTCTCTG GCTCTGCACA GAGGAAGAGA CCAGCAGTGG CTCCTCTCTC TGGAAAGGAG 
GCCAGAGAAC CCCTGCAGGA TGGCCCCACT GTGGACACAA ATGGACGGGC CAGCGCTGTC 
ACCAGCCCTG CTGAGAGAAC TCCAGGTTCA AGCGTGAATG GTGTGGAAGA GCCTAGCATT 
GCCAAGAGGC TCAGGGGCAC TCCCGAGCGG ATTGAGCTGG AGAACTACCG CCTGTCCGTG 
AAGCAGGCCG AGGAGCTGGA GGAGGTGCCC GAGGAGACAC AGGCCGAGCA CAACCTGAGC 
AGCGTGCTGG ACACGGGGGC AGAGGAGGAC GTGGCCAGCA GCAGTTCTGA GTCAGAGATG 
GAGGAAGAGG AGGAAGAAGA GCCTCCTCTG CCCACTTCGG ACCTGGGCGG TGTTCCTTGG 
AAGGAGGCCG TGAGGATCCA TGCTCTTCTG AAAGGAAAGA GTGAAGAGGA GCTAGAGGCC 
TCAAAGAGCT TTGGGCCTGG GCATGAAGAA GACGAGGAGG AGGAGGAGTA TGAAGAGGAG 
GAGGAGGAGG AGGAGGAAGA ATATGAAGAG GAAGAGGAAG AGGAAGAGTC TAGTGAAGTT 
GGGAGCCAGA GGCTACAGCA GATCGTAAAT CCGGCCGACC CCTTGGAGAT CCAGGCTGAT 
GTGCACTGGA CACATATCCG AGAGAGAGAG GAGGAGGAGC GAATGGTCCC AACCTCTGAG 
TCCTCCACTT CTAGAGTCCC ATTTGATGAG AATGACCTGG AGGAAGACGT GGACTCGGAG 
CCAGCAAAGA TAGAAGGGGA GGCCACAGAG AATGGGGACA CAGGGGACAC TGGTGCTGAG 
CTGGACGATG ATCAGCGCTG GTCTGACGGG ATCCCATCGG ATGCCGAGAC GGATCTCCGC 
GTGCAGCACC AGGAGGCGGT AGAGGCAGAC CTGGAGCTGA GGGTCTCAGA GAATGAGGAG 
GAGCCACCTG AGGTCACACC AGGGCATCCG GAGAGAAGTC TCACCCGGGT CCCCAGCCCT 
GCCCTGTCCC CTGAGGAGCA AGCTGGTCTG CGGTCCCCAG GCCACAGCCC TAAGGCAGAG 
GGGGCCCAAA TCCCACTTGT CTCTGTTGTC ACCAAGGCAA AATTGCCTGA AGAGAACCTT 
TTCCCCAAGC CTTTGCTCCC CAAAGTGAAG CCCAAGGTGG AAGTGCCCTG CAGTCAGAGA 
GCTGCATGCT CCCCCATCCG TTCTCAGCCA GTGGCTCTGC CAGAAGCCAG GACACCTACC 
TCCCCAGTGA GCTCGCAGTG CCTGTCGCCT TTGGCCACCT CCACCCCCAC CAGCACCCAG 
CTCCCCATTT GCTCCCAGCC TCAGCCTTCC TCTGAGACCA CGGCCCCATC CCCCACCAAG 
TCCCCCATAC GGCTCCAGCC TGTTTCAGCC AAAACATCTA TCCCAGTGGT TCCCCTCCCC 
TTGAAGAACC AAGGGGACAC CAAGGACAGG CTGGGCAGCC CTCTCGCTGT GGATGAGGCC 
CTGAAGCGGA ATGACCTGGT GGCAGAGTTC TGGATGAAGA GCGCCGAGAT CCGCCGCAGC 
CTCGGGCTCA CACCAGTGGA TCGGAGCAAG GTGTATGAGC CCAGCTTCCC TTCGCCTGCC 
TTTAAACCAG TGTCCCTAAA ATCCTATGCA GTGGAGAAGT CTCCCGAGGG GGAGGGACTC 
CAGCTTCTGA AGCCTCCACC TGTTCCTAAG AGACTAGGCC TGCCGAAGTC AGACGGTGAC 
CAGCCCTTCC TGCCAACCCC CAAGTCCCCG TCGGACAGAG AGCTGAAAAG CTCCCAGGAG 
GAGAGGAGAG ATCTGTCGAG CAGCTCCGGG CTGGGCCTTC ACGGGAGCTC CTCCAACATG 
AAGACTTTGG GCAGCCAGAG CTTCAACACA TCAGACTCCA CCATGCTCAC TCCCCCTTCA 
AGCCCACCGC CACCACCACC CCAAGACGAG GAACCCGCAA CTCTTCGGAG GAAGCCATAT 
CAGACTCATG AGCATAAGGA CGCTGAGCCC AAGGCCTCGG TGATTCCACC ACCACCACCT 
GCGACGTTCA TGCGGGCCCC CCGGGAGCCC ACCCAGCCCC TCCAAGAGGA GATGCGGAAG 
TCGTTTGTGG AGAGCGTGGA TGAGATCCCA TTTGCCGATG ATGTGGAGGA CACCTATGAT 
GACAAGACCG AGGACTCGAG TCTCCAGGAG AAGTTCTTCA CACCCCCCTC CTACTGGCCC 
CGCACGGAGA GGCCCCTCCA CCCACCCCTA GCCAAGGAGA ATGGTAGGTT GCCTACTCTG 
GAGAGTGGGG TCCAGCCACA GAAGAGGGGC CTGCCCTTAG TCTCTCCAGA AGCCAAGGAG 
CTGGCTGAGG AGCGCATGCG AGCCAGGGAG AAGTCTGTGA AGAGCCAGGC GTTGAGAGAC 
GCTATGGCCA AGCAGCTGAG TAAGATGAAG GAAATGGAGA TGGCGGCTGG GACCTCCAGT 
CCCCCAGGAG GCACCTCCCA CAAAGCCTCC TCTGTTCCCT CCAAGGGCAA AGACCTCAGC 
CCCGAGTCCC CTAGACGCCC AGTTCTCAAA GGCCCCAGGG AGCCTGCCCT GAAGCACGAA 
GCCACTAGTG AGGAGGTCCT CTCCCCACCA TCGGACTCAG GGGGCCCAGA TGGTTCGGTC 
ACCTCATCCG AGGGCTCCAG TGGGAAGAGC AAGAAGAGAT CGTCACTCTT CTCCCCCCGC 
AGAAACAAGA AAGAGAAGAA GTCCAAAGGT GAGGGCCGGC CCCCGGAGAA GCCCAGCCCA 
AGCCTTCTGG AGGAAGCTGC TGCCAAGCCC AAGTCCCTGT GGAAGTCGGT CTTTTCCGGG 
TACAAGAAGG ACAAAAAGAA GAAGGGTGAT GACAAGTCCT GTTCCAGCAC CCCTTCCAGC 
GGTGCCACTG TGGACTCTGG CAAGCACAAG ATGTCCCCTG TTGTGAGAGC AGAGCTGCAC 
CTGCGGCGCC AGTTGAGCTT CTCCGAGGAC TCAGACCTGT CCAGTGATGA CATCCTTGAG 
AGGTCCTCCC AGAAGTCCAG GCGAGAGCCG AGAACCTACA CAGAGGAGGA ACTGAATGCC 
AAGCTGACCC GGCGTGTGCA GAAGGCAGCT CGTAGACAGG CCAAGCAAGA GGAGCTGAAG 
CGGCTGCACC GAGCACAGAT CATCCAGAGG CAGCTGGAGC AGGTGGAGGA GAAGCAGAGA 
CAGCTGGAGG AAAGAGGGGT GGCCGTGGAG AAGGCGCTCC GTGGTGAAGC AGGCATGGGC 
AAGAAGGATG ACCCCAAGCT GATGCAGGAG TGGTTCAAGT TGGTGCAAGA GAAGAATGCC 
ATGGTGCGCT ATGAGTCGGA GCTGATGATT TTTGCCCGGG AGCTGGAACT GGAGGACCGG 
CAGAGCCGAC TGCAGCAGGA GCTCCGGGAG CGGATGGCGG TGGAAGATCA CCTGAAGACT 
GAGGAGGAGC TGTCTGAGGA GAAGAAGATC TTGAACGAGA TGCTGGAGGT GGTGGAGCAG 
AGAGATGCCC TCGTGGCCCT GCTTGAGGAG CAGCGACTCC GGGAGAAGGA GGAGGACAAA 
GACCTGGAGG CTGTCATGCT GTCCAAGGGC TTCAGCCTGA ACTGGTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_022410876.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022266584.1 Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X7, mRNA.

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CloneID	OCe282672
Clone ID Related Accession (Same CDS sequence)	XM_022410876.1
Accession Version	XM_022410876.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	F-actin-methionine sulfoxide oxidase MICAL3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGTTC TGAGTCAGAG ATGGAGGAAG AGGAGGAAGA AGAGCCTCCT 
CTGCCCACTT CGGACCTGGG CGGTGTTCCT TGGAAGGAGG CCGTGAGGAT CCATGCTCTT 
CTGAAAGGAA AGAGTGAAGA GGAGCTAGAG GCCTCAAAGA GCTTTGGGCC TGGGCATGAA 
GAAGACGAGG AGGAGGAGGA GTATGAAGAG GAGGAGGAGG AGGAGGAGGA AGAATATGAA 
GAGGAAGAGG AAGAGGAAGA GTCTAGTGAA GAAGGGGAGT ATTGCCCCTG GGACAGAGAA 
CTCCTACAAG GCCAGTGGCT CCGGCAGCTG TCACAGGAAG AAACTACGGG TACACGTAAA 
GTTGGGAGCC AGAGGCTACA GCAGATCGTA AATCCGGCCG ACCCCTTGGA GATCCAGGCT 
GATGTGCACT GGACACATAT CCGAGAGAGA GAGGAGGAGG AGCGAATGGT CCCAACCTCT 
GAGTCCTCCA CTTCTAGAGT GCCTGACCTT CCCTCCATAC GCCGCGCAGC AGTGCAGGCC 
TGGCTGGAGA CGGTCTCTGG AGTCCCATTT GATGAGAATG ACCTGGAGGA AGACGTGGAC 
TCGGAGCCAG CAAAGATAGA AGGGGAGGCC ACAGAGAATG GGGACACAGG GGACACTGGT 
GCTGAGCTGG ACGATGATCA GCGCTGGTCT GACGGGATCC CATCGGATGC CGAGACGGAT 
CTCCGCGTGC AGCACCAGGA GGCGGTAGAG GCAGACCTGG AGCTGAGGGT CTCAGAGAAT 
GAGGAGGAGC CACCTGAGGT CACACCAGGG CATCCGGAGA GAAGTCTCAC CCGGGTCCCC 
AGCCCTGCCC TGTCCCCTGA GGAGCAAGCT GGTCTGCGGT CCCCAGGCCA CAGCCCTAAG 
GCAGAGGGGG CCCAAATCCC ACTTGTCTCT GTTGTCACCA AGGCAAAATT GCCTGAAGAG 
AACCTTTTCC CCAAGCCTTT GCTCCCCAAA GTGAAGCCCA AGGTGGAAGT GCCCTGCAGT 
CAGAGAGCTG CATGCTCCCC CATCCGTTCT CAGCCAGTGG CTCTGCCAGA AGCCAGGACA 
CCTACCTCCC CAGTGAGCTC GCAGTGCCTG TCGCCTTTGG CCACCTCCAC CCCCACCAGC 
ACCCAGCTCC CCATTTGCTC CCAGCCTCAG CCTTCCTCTG AGACCACGGC CCCATCCCCC 
ACCAAGTCCC CCATACGGCT CCAGCCTGTT TCAGCCAAAA CATCTATCCC AGTGGTTCCC 
CTCCCCTTGA AGAACCAAGG GGACACCAAG GACAGGCTGG GCAGCCCTCT CGCTGTGGAT 
GAGGCCCTGA AGCGGAATGA CCTGGTGGCA GAGTTCTGGA TGAAGAGCGC CGAGATCCGC 
CGCAGCCTCG GGCTCACACC AGTGGATCGG AGCAAGGTGT ATGAGCCCAG CTTCCCTTCG 
CCTGCCTTTA AACCAGTGTC CCTAAAATCC TATGCAGTGG AGAAGTCTCC CGAGGGGGAG 
GGACTCCAGC TTCTGAAGCC TCCACCTGTT CCTAAGAGAC TAGGCCTGCC GAAGTCAGAC 
GGTGACCAGC CCTTCCTGCC AACCCCCAAG TCCCCGTCGG ACAGAGAGCT GAAAAGCTCC 
CAGGAGGAGA GGAGAGATCT GTCGAGCAGC TCCGGGCTGG GCCTTCACGG GAGCTCCTCC 
AACATGAAGA CTTTGGGCAG CCAGAGCTTC AACACATCAG ACTCCACCAT GCTCACTCCC 
CCTTCAAGCC CACCGCCACC ACCACCCCAA GACGAGGAAC CCGCAACTCT TCGGAGGAAG 
CCATATCAGA CTCATGAGCA TAAGGACGCT GAGCCCAAGG CCTCGGTGAT TCCACCACCA 
CCACCTGCGA CGTTCATGCG GGCCCCCCGG GAGCCCACCC AGCCCCTCCA AGAGGAGATG 
CGGAAGTCGT TTGTGGAGAG CGTGGATGAG ATCCCATTTG CCGATGATGT GGAGGACACC 
TATGATGACA AGACCGAGGA CTCGAGTCTC CAGGAGAAGT TCTTCACACC CCCCTCCTAC 
TGGCCCCGCA CGGAGAGGCC CCTCCACCCA CCCCTAGCCA AGGAGAATGG TAGGTTGCCT 
ACTCTGGAGA GTGGGGTCCA GCCACAGAAG AGGGGCCTGC CCTTAGTCTC TCCAGAAGCC 
AAGGAGCTGG CTGAGGAGCG CATGCGAGCC AGGGAGAAGT CTGTGAAGAG CCAGGCGTTG 
AGAGACGCTA TGGCCAAGCA GCTGAGTAAG ATGAAGGAAA TGGAGATGGC GGCTGGGACC 
TCCAGTCCCC CAGGAGGCAC CTCCCACAAA GCCTCCTCTG TTCCCTCCAA GGGCAAAGAC 
CTCAGCCCCG AGTCCCCTAG ACGCCCAGTT CTCAAAGGCC CCAGGGAGCC TGCCCTGAAG 
CACGAAGCCA CTAGTGAGGA GGTCCTCTCC CCACCATCGG ACTCAGGGGG CCCAGATGGT 
TCGGTCACCT CATCCGAGGG CTCCAGTGGG AAGAGCAAGA AGAGATCGTC ACTCTTCTCC 
CCCCGCAGAA ACAAGAAAGA GAAGAAGTCC AAAGGTGAGG GCCGGCCCCC GGAGAAGCCC 
AGCCCAAGCC TTCTGGAGGA AGCTGCTGCC AAGCCCAAGT CCCTGTGGAA GTCGGTCTTT 
TCCGGGTACA AGAAGGACAA AAAGAAGAAG GGTGATGACA AGTCCTGTTC CAGCACCCCT 
TCCAGCGGTG CCACTGTGGA CTCTGGCAAG CACAAGATGT CCCCTGTTGT GAGAGCAGAG 
CTGCACCTGC GGCGCCAGTT GAGCTTCTCC GAGGACTCAG ACCTGTCCAG TGATGACATC 
CTTGAGAGGT CCTCCCAGAA GTCCAGGCGA GAGTCGGTTT ATGTACCACA CGCCTTGGCA 
TTCAAGAGAT CATATCCATC AAAGCCGAGA ACCTACACAG AGGAGGAACT GAATGCCAAG 
CTGACCCGGC GTGTGCAGAA GGCAGCTCGT AGACAGGCCA AGCAAGAGGA GCTGAAGCGG 
CTGCACCGAG CACAGATCAT CCAGAGGCAG CTGGAGCAGG TGGAGGAGAA GCAGAGACAG 
CTGGAGGAAA GAGGGGTGGC CGTGGAGAAG GCGCTCCGTG GTGAAGCAGA CTATTGGGGA 
GAGTCTTATT ACAGTGACCT CATCGACTTG CATCTGGGTG TTGAGCCCAG TGGCGGGACT 
CCACGGCGTA GACCTCTCTC CTTCTGCCCT TGCTGTGTCC AGGAAGGCAT GGGCAAGAAG 
GATGACCCCA AGCTGATGCA GGAGTGGTTC AAGTTGGTGC AAGAGAAGAA TGCCATGGTG 
CGCTATGAGT CGGAGCTGAT GATTTTTGCC CGGGAGCTGG AACTGGAGGA CCGGCAGAGC 
CGACTGCAGC AGGAGCTCCG GGAGCGGATG GCGGTGGAAG ATCACCTGAA GACTGAGGAG 
GAGCTGTCTG AGGAGAAGAA GATCTTGAAC GAGATGCTGG AGGTGGTGGA GCAGAGAGAT 
GCCCTCGTGG CCCTGCTTGA GGAGCAGCGA CTCCGGGAGA AGGAGGAGGA CAAAGACCTG 
GAGGCTGTCA TGCTGTCCAA GGGCTTCAGC CTGAACTGGT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022266584.1
CDS	258..7082
Translation	MEDSKNETVNHAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDYRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKACTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFQGLVQPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELKEATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLNYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREQNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNININFLRPSQVRHLYNTGETRDIHLEMENLVNSRTTPKLTRNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGKEMASVGEPDKLSMVMYLTQFYEMFKDSLPSSDALDLNAEEKAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEEAPRGYRGGRPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSTGMRRQPTQERGVSQPSCCLPEQGHPAPTPQWKQQQEKERSRTCPKKVITLSPPLTPPPCRAPRSQQTYRDLDADSRGKQSPHHERPEPEPSRRFFVDQWELSLSLRSSNRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCRKRVYVMERLSAEGKFFHRSCFKCEYCATTLRLSAYAYDIEDGKFYCKPHYCYRLSGSAQRKRPAVAPLSGKEAREPLQDGPTVDTNGRASAVTSPAERTPGSSVNGVEEPSIAKRLRGTPERIELENYRLSVKQAEELEEVPEETQAEHNLSSVLDTGAEEDVASSSESEMEEEEEEEPPLPTSDLGGVPWKEAVRIHALLKGKSEEELEASKSFGPGHEEDEEEEEYEEEEEEEEEEYEEEEEEEESSEEGEYCPWDRELLQGQWLRQLSQEETTGTRKVGSQRLQQIVNPADPLEIQADVHWTHIREREEEERMVPTSESSTSRVPDLPSIRRAAVQAWLETVSGVPFDENDLEEDVDSEPAKIEGEATENGDTGDTGAELDDDQRWSDGIPSDAETDLRVQHQEAVEADLELRVSENEEEPPEVTPGHPERSLTRVPSPALSPEEQAGLRSPGHSPKAEGAQIPLVSVVTKAKLPEENLFPKPLLPKVKPKVEVPCSQRAACSPIRSQPVALPEARTPTSPVSSQCLSPLATSTPTSTQLPICSQPQPSSETTAPSPTKSPIRLQPVSAKTSIPVVPLPLKNQGDTKDRLGSPLAVDEALKRNDLVAEFWMKSAEIRRSLGLTPVDRSKVYEPSFPSPAFKPVSLKSYAVEKSPEGEGLQLLKPPPVPKRLGLPKSDGDQPFLPTPKSPSDRELKSSQEERRDLSSSSGLGLHGSSSNMKTLGSQSFNTSDSTMLTPPSSPPPPPPQDEEPATLRRKPYQTHEHKDAEPKASVIPPPPPATFMRAPREPTQPLQEEMRKSFVESVDEIPFADDVEDTYDDKTEDSSLQEKFFTPPSYWPRTERPLHPPLAKENGRLPTLESGVQPQKRGLPLVSPEAKELAEERMRAREKSVKSQALRDAMAKQLSKMKEMEMAAGTSSPPGGTSHKASSVPSKGKDLSPESPRRPVLKGPREPALKHEATSEEVLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRNKKEKKSKGEGRPPEKPSPSLLEEAAAKPKSLWKSVFSGYKKDKKKKGDDKSCSSTPSSGATVDSGKHKMSPVVRAELHLRRQLSFSEDSDLSSDDILERSSQKSRRESVYVPHALAFKRSYPSKPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELSEEKKILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAVMLSKGFSLNWS

Target ORF information:

RefSeq Version	XM_022410876.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022410876.1

ORF Insert Sequence:

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGTTC TGAGTCAGAG ATGGAGGAAG AGGAGGAAGA AGAGCCTCCT 
CTGCCCACTT CGGACCTGGG CGGTGTTCCT TGGAAGGAGG CCGTGAGGAT CCATGCTCTT 
CTGAAAGGAA AGAGTGAAGA GGAGCTAGAG GCCTCAAAGA GCTTTGGGCC TGGGCATGAA 
GAAGACGAGG AGGAGGAGGA GTATGAAGAG GAGGAGGAGG AGGAGGAGGA AGAATATGAA 
GAGGAAGAGG AAGAGGAAGA GTCTAGTGAA GAAGGGGAGT ATTGCCCCTG GGACAGAGAA 
CTCCTACAAG GCCAGTGGCT CCGGCAGCTG TCACAGGAAG AAACTACGGG TACACGTAAA 
GTTGGGAGCC AGAGGCTACA GCAGATCGTA AATCCGGCCG ACCCCTTGGA GATCCAGGCT 
GATGTGCACT GGACACATAT CCGAGAGAGA GAGGAGGAGG AGCGAATGGT CCCAACCTCT 
GAGTCCTCCA CTTCTAGAGT GCCTGACCTT CCCTCCATAC GCCGCGCAGC AGTGCAGGCC 
TGGCTGGAGA CGGTCTCTGG AGTCCCATTT GATGAGAATG ACCTGGAGGA AGACGTGGAC 
TCGGAGCCAG CAAAGATAGA AGGGGAGGCC ACAGAGAATG GGGACACAGG GGACACTGGT 
GCTGAGCTGG ACGATGATCA GCGCTGGTCT GACGGGATCC CATCGGATGC CGAGACGGAT 
CTCCGCGTGC AGCACCAGGA GGCGGTAGAG GCAGACCTGG AGCTGAGGGT CTCAGAGAAT 
GAGGAGGAGC CACCTGAGGT CACACCAGGG CATCCGGAGA GAAGTCTCAC CCGGGTCCCC 
AGCCCTGCCC TGTCCCCTGA GGAGCAAGCT GGTCTGCGGT CCCCAGGCCA CAGCCCTAAG 
GCAGAGGGGG CCCAAATCCC ACTTGTCTCT GTTGTCACCA AGGCAAAATT GCCTGAAGAG 
AACCTTTTCC CCAAGCCTTT GCTCCCCAAA GTGAAGCCCA AGGTGGAAGT GCCCTGCAGT 
CAGAGAGCTG CATGCTCCCC CATCCGTTCT CAGCCAGTGG CTCTGCCAGA AGCCAGGACA 
CCTACCTCCC CAGTGAGCTC GCAGTGCCTG TCGCCTTTGG CCACCTCCAC CCCCACCAGC 
ACCCAGCTCC CCATTTGCTC CCAGCCTCAG CCTTCCTCTG AGACCACGGC CCCATCCCCC 
ACCAAGTCCC CCATACGGCT CCAGCCTGTT TCAGCCAAAA CATCTATCCC AGTGGTTCCC 
CTCCCCTTGA AGAACCAAGG GGACACCAAG GACAGGCTGG GCAGCCCTCT CGCTGTGGAT 
GAGGCCCTGA AGCGGAATGA CCTGGTGGCA GAGTTCTGGA TGAAGAGCGC CGAGATCCGC 
CGCAGCCTCG GGCTCACACC AGTGGATCGG AGCAAGGTGT ATGAGCCCAG CTTCCCTTCG 
CCTGCCTTTA AACCAGTGTC CCTAAAATCC TATGCAGTGG AGAAGTCTCC CGAGGGGGAG 
GGACTCCAGC TTCTGAAGCC TCCACCTGTT CCTAAGAGAC TAGGCCTGCC GAAGTCAGAC 
GGTGACCAGC CCTTCCTGCC AACCCCCAAG TCCCCGTCGG ACAGAGAGCT GAAAAGCTCC 
CAGGAGGAGA GGAGAGATCT GTCGAGCAGC TCCGGGCTGG GCCTTCACGG GAGCTCCTCC 
AACATGAAGA CTTTGGGCAG CCAGAGCTTC AACACATCAG ACTCCACCAT GCTCACTCCC 
CCTTCAAGCC CACCGCCACC ACCACCCCAA GACGAGGAAC CCGCAACTCT TCGGAGGAAG 
CCATATCAGA CTCATGAGCA TAAGGACGCT GAGCCCAAGG CCTCGGTGAT TCCACCACCA 
CCACCTGCGA CGTTCATGCG GGCCCCCCGG GAGCCCACCC AGCCCCTCCA AGAGGAGATG 
CGGAAGTCGT TTGTGGAGAG CGTGGATGAG ATCCCATTTG CCGATGATGT GGAGGACACC 
TATGATGACA AGACCGAGGA CTCGAGTCTC CAGGAGAAGT TCTTCACACC CCCCTCCTAC 
TGGCCCCGCA CGGAGAGGCC CCTCCACCCA CCCCTAGCCA AGGAGAATGG TAGGTTGCCT 
ACTCTGGAGA GTGGGGTCCA GCCACAGAAG AGGGGCCTGC CCTTAGTCTC TCCAGAAGCC 
AAGGAGCTGG CTGAGGAGCG CATGCGAGCC AGGGAGAAGT CTGTGAAGAG CCAGGCGTTG 
AGAGACGCTA TGGCCAAGCA GCTGAGTAAG ATGAAGGAAA TGGAGATGGC GGCTGGGACC 
TCCAGTCCCC CAGGAGGCAC CTCCCACAAA GCCTCCTCTG TTCCCTCCAA GGGCAAAGAC 
CTCAGCCCCG AGTCCCCTAG ACGCCCAGTT CTCAAAGGCC CCAGGGAGCC TGCCCTGAAG 
CACGAAGCCA CTAGTGAGGA GGTCCTCTCC CCACCATCGG ACTCAGGGGG CCCAGATGGT 
TCGGTCACCT CATCCGAGGG CTCCAGTGGG AAGAGCAAGA AGAGATCGTC ACTCTTCTCC 
CCCCGCAGAA ACAAGAAAGA GAAGAAGTCC AAAGGTGAGG GCCGGCCCCC GGAGAAGCCC 
AGCCCAAGCC TTCTGGAGGA AGCTGCTGCC AAGCCCAAGT CCCTGTGGAA GTCGGTCTTT 
TCCGGGTACA AGAAGGACAA AAAGAAGAAG GGTGATGACA AGTCCTGTTC CAGCACCCCT 
TCCAGCGGTG CCACTGTGGA CTCTGGCAAG CACAAGATGT CCCCTGTTGT GAGAGCAGAG 
CTGCACCTGC GGCGCCAGTT GAGCTTCTCC GAGGACTCAG ACCTGTCCAG TGATGACATC 
CTTGAGAGGT CCTCCCAGAA GTCCAGGCGA GAGTCGGTTT ATGTACCACA CGCCTTGGCA 
TTCAAGAGAT CATATCCATC AAAGCCGAGA ACCTACACAG AGGAGGAACT GAATGCCAAG 
CTGACCCGGC GTGTGCAGAA GGCAGCTCGT AGACAGGCCA AGCAAGAGGA GCTGAAGCGG 
CTGCACCGAG CACAGATCAT CCAGAGGCAG CTGGAGCAGG TGGAGGAGAA GCAGAGACAG 
CTGGAGGAAA GAGGGGTGGC CGTGGAGAAG GCGCTCCGTG GTGAAGCAGA CTATTGGGGA 
GAGTCTTATT ACAGTGACCT CATCGACTTG CATCTGGGTG TTGAGCCCAG TGGCGGGACT 
CCACGGCGTA GACCTCTCTC CTTCTGCCCT TGCTGTGTCC AGGAAGGCAT GGGCAAGAAG 
GATGACCCCA AGCTGATGCA GGAGTGGTTC AAGTTGGTGC AAGAGAAGAA TGCCATGGTG 
CGCTATGAGT CGGAGCTGAT GATTTTTGCC CGGGAGCTGG AACTGGAGGA CCGGCAGAGC 
CGACTGCAGC AGGAGCTCCG GGAGCGGATG GCGGTGGAAG ATCACCTGAA GACTGAGGAG 
GAGCTGTCTG AGGAGAAGAA GATCTTGAAC GAGATGCTGG AGGTGGTGGA GCAGAGAGAT 
GCCCTCGTGG CCCTGCTTGA GGAGCAGCGA CTCCGGGAGA AGGAGGAGGA CAAAGACCTG 
GAGGCTGTCA TGCTGTCCAA GGGCTTCAGC CTGAACTGGT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_022410877.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022266585.1 Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X8, mRNA.

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CloneID	OCe282673
Clone ID Related Accession (Same CDS sequence)	XM_022410877.1
Accession Version	XM_022410877.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	F-actin-methionine sulfoxide oxidase MICAL3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTGCCTGAC CTTCCCTCCA TACGCCGCGC AGCAGTGCAG 
GCCTGGCTGG AGACGGTCTC TGGAGTCCCA TTTGATGAGA ATGACCTGGA GGAAGACGTG 
GACTCGGAGC CAGCAAAGAT AGAAGGGGAG GCCACAGAGA ATGGGGACAC AGGGGACACT 
GGTGCTGAGC TGGACGATGA TCAGCGCTGG TCTGACGGGA TCCCATCGGA TGCCGAGACG 
GATCTCCGCG TGCAGCACCA GGAGGCGGTA GAGGCAGACC TGGAGCTGAG GGTCTCAGAG 
AATGAGGAGG AGCCACCTGA GGTCACACCA GGGCATCCGG AGAGAAGTCT CACCCGGGTC 
CCCAGCCCTG CCCTGTCCCC TGAGGAGCAA GCTGGTCTGC GGTCCCCAGG CCACAGCCCT 
AAGGCAGAGG GGGCCCAAAT CCCACTTGTC TCTGTTGTCA CCAAGGCAAA ATTGCCTGAA 
GAGAACCTTT TCCCCAAGCC TTTGCTCCCC AAAGTGAAGC CCAAGGTGGA AGTGCCCTGC 
AGTCAGAGAG CTGCATGCTC CCCCATCCGT TCTCAGCCAG TGGCTCTGCC AGAAGCCAGG 
ACACCTACCT CCCCAGTGAG CTCGCAGTGC CTGTCGCCTT TGGCCACCTC CACCCCCACC 
AGCACCCAGC TCCCCATTTG CTCCCAGCCT CAGCCTTCCT CTGAGACCAC GGCCCCATCC 
CCCACCAAGT CCCCCATACG GCTCCAGCCT GTTTCAGCCA AAACATCTAT CCCAGTGGTT 
CCCCTCCCCT TGAAGAACCA AGGGGACACC AAGGACAGGC TGGGCAGCCC TCTCGCTGTG 
GATGAGGCCC TGAAGCGGAA TGACCTGGTG GCAGAGTTCT GGATGAAGAG CGCCGAGATC 
CGCCGCAGCC TCGGGCTCAC ACCAGTGGAT CGGAGCAAGG TGTATGAGCC CAGCTTCCCT 
TCGCCTGCCT TTAAACCAGT GTCCCTAAAA TCCTATGCAG TGGAGAAGTC TCCCGAGGGG 
GAGGGACTCC AGCTTCTGAA GCCTCCACCT GTTCCTAAGA GACTAGGCCT GCCGAAGTCA 
GACGGTGACC AGCCCTTCCT GCCAACCCCC AAGTCCCCGT CGGACAGAGA GCTGAAAAGC 
TCCCAGGAGG AGAGGAGAGA TCTGTCGAGC AGCTCCGGGC TGGGCCTTCA CGGGAGCTCC 
TCCAACATGA AGACTTTGGG CAGCCAGAGC TTCAACACAT CAGACTCCAC CATGCTCACT 
CCCCCTTCAA GCCCACCGCC ACCACCACCC CAAGACGAGG AACCCGCAAC TCTTCGGAGG 
AAGCCATATC AGACTCATGA GCATAAGGAC GCTGAGCCCA AGGCCTCGGT GATTCCACCA 
CCACCACCTG CGACGTTCAT GCGGGCCCCC CGGGAGCCCA CCCAGCCCCT CCAAGAGGAG 
ATGCGGAAGT CGTTTGTGGA GAGCGTGGAT GAGATCCCAT TTGCCGATGA TGTGGAGGAC 
ACCTATGATG ACAAGACCGA GGACTCGAGT CTCCAGGAGA AGTTCTTCAC ACCCCCCTCC 
TACTGGCCCC GCACGGAGAG GCCCCTCCAC CCACCCCTAG CCAAGGAGAA TGGTAGGTTG 
CCTACTCTGG AGAGTGGGGT CCAGCCACAG AAGAGGGGCC TGCCCTTAGT CTCTCCAGAA 
GCCAAGGAGC TGGCTGAGGA GCGCATGCGA GCCAGGGAGA AGTCTGTGAA GAGCCAGGCG 
TTGAGAGACG CTATGGCCAA GCAGCTGAGT AAGATGAAGG AAATGGAGAT GGCGGCTGGG 
ACCTCCAGTC CCCCAGGAGG CACCTCCCAC AAAGCCTCCT CTGTTCCCTC CAAGGGCAAA 
GACCTCAGCC CCGAGTCCCC TAGACGCCCA GTTCTCAAAG GCCCCAGGGA GCCTGCCCTG 
AAGCACGAAG CCACTAGTGA GGAGGTCCTC TCCCCACCAT CGGACTCAGG GGGCCCAGAT 
GGTTCGGTCA CCTCATCCGA GGGCTCCAGT GGGAAGAGCA AGAAGAGATC GTCACTCTTC 
TCCCCCCGCA GAAACAAGAA AGAGAAGAAG TCCAAAGGTG AGGGCCGGCC CCCGGAGAAG 
CCCAGCCCAA GCCTTCTGGA GGAAGCTGCT GCCAAGCCCA AGTCCCTGTG GAAGTCGGTC 
TTTTCCGGGT ACAAGAAGGA CAAAAAGAAG AAGGGTGATG ACAAGTCCTG TTCCAGCACC 
CCTTCCAGCG GTGCCACTGT GGACTCTGGC AAGCACAAGA TGTCCCCTGT TGTGAGAGCA 
GAGCTGCACC TGCGGCGCCA GTTGAGCTTC TCCGAGGACT CAGACCTGTC CAGTGATGAC 
ATCCTTGAGA GGTCCTCCCA GAAGTCCAGG CGAGAGCCGA GAACCTACAC AGAGGAGGAA 
CTGAATGCCA AGCTGACCCG GCGTGTGCAG AAGGCAGCTC GTAGACAGGC CAAGCAAGAG 
GAGCTGAAGC GGCTGCACCG AGCACAGATC ATCCAGAGGC AGCTGGAGCA GGTGGAGGAG 
AAGCAGAGAC AGCTGGAGGA AAGAGGGGTG GCCGTGGAGA AGGCGCTCCG TGGTGAAGCA 
GACTATTGGG GAGAGTCTTA TTACAGTGAC CTCATCGACT TGCATCTGGG TGTTGAGCCC 
AGTGGCGGGA CTCCACGGCG TAGACCTCTC TCCTTCTGCC CTTGCTGTGT CCAGGAAGGC 
ATGGGCAAGA AGGATGACCC CAAGCTGATG CAGGAGTGGT TCAAGTTGGT GCAAGAGAAG 
AATGCCATGG TGCGCTATGA GTCGGAGCTG ATGATTTTTG CCCGGGAGCT GGAACTGGAG 
GACCGGCAGA GCCGACTGCA GCAGGAGCTC CGGGAGCGGA TGGCGGTGGA AGATCACCTG 
AAGACTGAGG AGGAGCTGTC TGAGGAGAAG AAGATCTTGA ACGAGATGCT GGAGGTGGTG 
GAGCAGAGAG ATGCCCTCGT GGCCCTGCTT GAGGAGCAGC GACTCCGGGA GAAGGAGGAG 
GACAAAGACC TGGAGGCTGT CATGCTGTCC AAGGGCTTCA GCCTGAACTG GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022266585.1
CDS	258..7034
Translation	MEDSKNETVNHAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDYRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKACTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFQGLVQPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELKEATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLNYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREQNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNININFLRPSQVRHLYNTGETRDIHLEMENLVNSRTTPKLTRNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGKEMASVGEPDKLSMVMYLTQFYEMFKDSLPSSDALDLNAEEKAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEEAPRGYRGGRPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSTGMRRQPTQERGVSQPSCCLPEQGHPAPTPQWKQQQEKERSRTCPKKVITLSPPLTPPPCRAPRSQQTYRDLDADSRGKQSPHHERPEPEPSRRFFVDQWELSLSLRSSNRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCRKRVYVMERLSAEGKFFHRSCFKCEYCATTLRLSAYAYDIEDGKFYCKPHYCYRLSGSAQRKRPAVAPLSGKEAREPLQDGPTVDTNGRASAVTSPAERTPGSSVNGVEEPSIAKRLRGTPERIELENYRLSVKQAEELEEVPEETQAEHNLSSVLDTGAEEDVASSSSESEMEEEEEEEPPLPTSDLGGVPWKEAVRIHALLKGKSEEELEASKSFGPGHEEDEEEEEYEEEEEEEEEEYEEEEEEEESSEEGEYCPWDRELLQGQWLRQLSQEETTGTRKVGSQRLQQIVNPADPLEIQADVHWTHIREREEEERMVPTSESSTSRVPDLPSIRRAAVQAWLETVSGVPFDENDLEEDVDSEPAKIEGEATENGDTGDTGAELDDDQRWSDGIPSDAETDLRVQHQEAVEADLELRVSENEEEPPEVTPGHPERSLTRVPSPALSPEEQAGLRSPGHSPKAEGAQIPLVSVVTKAKLPEENLFPKPLLPKVKPKVEVPCSQRAACSPIRSQPVALPEARTPTSPVSSQCLSPLATSTPTSTQLPICSQPQPSSETTAPSPTKSPIRLQPVSAKTSIPVVPLPLKNQGDTKDRLGSPLAVDEALKRNDLVAEFWMKSAEIRRSLGLTPVDRSKVYEPSFPSPAFKPVSLKSYAVEKSPEGEGLQLLKPPPVPKRLGLPKSDGDQPFLPTPKSPSDRELKSSQEERRDLSSSSGLGLHGSSSNMKTLGSQSFNTSDSTMLTPPSSPPPPPPQDEEPATLRRKPYQTHEHKDAEPKASVIPPPPPATFMRAPREPTQPLQEEMRKSFVESVDEIPFADDVEDTYDDKTEDSSLQEKFFTPPSYWPRTERPLHPPLAKENGRLPTLESGVQPQKRGLPLVSPEAKELAEERMRAREKSVKSQALRDAMAKQLSKMKEMEMAAGTSSPPGGTSHKASSVPSKGKDLSPESPRRPVLKGPREPALKHEATSEEVLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRNKKEKKSKGEGRPPEKPSPSLLEEAAAKPKSLWKSVFSGYKKDKKKKGDDKSCSSTPSSGATVDSGKHKMSPVVRAELHLRRQLSFSEDSDLSSDDILERSSQKSRREPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELSEEKKILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAVMLSKGFSLNWS

Target ORF information:

RefSeq Version	XM_022410877.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022410877.1

ORF Insert Sequence:

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTGCCTGAC CTTCCCTCCA TACGCCGCGC AGCAGTGCAG 
GCCTGGCTGG AGACGGTCTC TGGAGTCCCA TTTGATGAGA ATGACCTGGA GGAAGACGTG 
GACTCGGAGC CAGCAAAGAT AGAAGGGGAG GCCACAGAGA ATGGGGACAC AGGGGACACT 
GGTGCTGAGC TGGACGATGA TCAGCGCTGG TCTGACGGGA TCCCATCGGA TGCCGAGACG 
GATCTCCGCG TGCAGCACCA GGAGGCGGTA GAGGCAGACC TGGAGCTGAG GGTCTCAGAG 
AATGAGGAGG AGCCACCTGA GGTCACACCA GGGCATCCGG AGAGAAGTCT CACCCGGGTC 
CCCAGCCCTG CCCTGTCCCC TGAGGAGCAA GCTGGTCTGC GGTCCCCAGG CCACAGCCCT 
AAGGCAGAGG GGGCCCAAAT CCCACTTGTC TCTGTTGTCA CCAAGGCAAA ATTGCCTGAA 
GAGAACCTTT TCCCCAAGCC TTTGCTCCCC AAAGTGAAGC CCAAGGTGGA AGTGCCCTGC 
AGTCAGAGAG CTGCATGCTC CCCCATCCGT TCTCAGCCAG TGGCTCTGCC AGAAGCCAGG 
ACACCTACCT CCCCAGTGAG CTCGCAGTGC CTGTCGCCTT TGGCCACCTC CACCCCCACC 
AGCACCCAGC TCCCCATTTG CTCCCAGCCT CAGCCTTCCT CTGAGACCAC GGCCCCATCC 
CCCACCAAGT CCCCCATACG GCTCCAGCCT GTTTCAGCCA AAACATCTAT CCCAGTGGTT 
CCCCTCCCCT TGAAGAACCA AGGGGACACC AAGGACAGGC TGGGCAGCCC TCTCGCTGTG 
GATGAGGCCC TGAAGCGGAA TGACCTGGTG GCAGAGTTCT GGATGAAGAG CGCCGAGATC 
CGCCGCAGCC TCGGGCTCAC ACCAGTGGAT CGGAGCAAGG TGTATGAGCC CAGCTTCCCT 
TCGCCTGCCT TTAAACCAGT GTCCCTAAAA TCCTATGCAG TGGAGAAGTC TCCCGAGGGG 
GAGGGACTCC AGCTTCTGAA GCCTCCACCT GTTCCTAAGA GACTAGGCCT GCCGAAGTCA 
GACGGTGACC AGCCCTTCCT GCCAACCCCC AAGTCCCCGT CGGACAGAGA GCTGAAAAGC 
TCCCAGGAGG AGAGGAGAGA TCTGTCGAGC AGCTCCGGGC TGGGCCTTCA CGGGAGCTCC 
TCCAACATGA AGACTTTGGG CAGCCAGAGC TTCAACACAT CAGACTCCAC CATGCTCACT 
CCCCCTTCAA GCCCACCGCC ACCACCACCC CAAGACGAGG AACCCGCAAC TCTTCGGAGG 
AAGCCATATC AGACTCATGA GCATAAGGAC GCTGAGCCCA AGGCCTCGGT GATTCCACCA 
CCACCACCTG CGACGTTCAT GCGGGCCCCC CGGGAGCCCA CCCAGCCCCT CCAAGAGGAG 
ATGCGGAAGT CGTTTGTGGA GAGCGTGGAT GAGATCCCAT TTGCCGATGA TGTGGAGGAC 
ACCTATGATG ACAAGACCGA GGACTCGAGT CTCCAGGAGA AGTTCTTCAC ACCCCCCTCC 
TACTGGCCCC GCACGGAGAG GCCCCTCCAC CCACCCCTAG CCAAGGAGAA TGGTAGGTTG 
CCTACTCTGG AGAGTGGGGT CCAGCCACAG AAGAGGGGCC TGCCCTTAGT CTCTCCAGAA 
GCCAAGGAGC TGGCTGAGGA GCGCATGCGA GCCAGGGAGA AGTCTGTGAA GAGCCAGGCG 
TTGAGAGACG CTATGGCCAA GCAGCTGAGT AAGATGAAGG AAATGGAGAT GGCGGCTGGG 
ACCTCCAGTC CCCCAGGAGG CACCTCCCAC AAAGCCTCCT CTGTTCCCTC CAAGGGCAAA 
GACCTCAGCC CCGAGTCCCC TAGACGCCCA GTTCTCAAAG GCCCCAGGGA GCCTGCCCTG 
AAGCACGAAG CCACTAGTGA GGAGGTCCTC TCCCCACCAT CGGACTCAGG GGGCCCAGAT 
GGTTCGGTCA CCTCATCCGA GGGCTCCAGT GGGAAGAGCA AGAAGAGATC GTCACTCTTC 
TCCCCCCGCA GAAACAAGAA AGAGAAGAAG TCCAAAGGTG AGGGCCGGCC CCCGGAGAAG 
CCCAGCCCAA GCCTTCTGGA GGAAGCTGCT GCCAAGCCCA AGTCCCTGTG GAAGTCGGTC 
TTTTCCGGGT ACAAGAAGGA CAAAAAGAAG AAGGGTGATG ACAAGTCCTG TTCCAGCACC 
CCTTCCAGCG GTGCCACTGT GGACTCTGGC AAGCACAAGA TGTCCCCTGT TGTGAGAGCA 
GAGCTGCACC TGCGGCGCCA GTTGAGCTTC TCCGAGGACT CAGACCTGTC CAGTGATGAC 
ATCCTTGAGA GGTCCTCCCA GAAGTCCAGG CGAGAGCCGA GAACCTACAC AGAGGAGGAA 
CTGAATGCCA AGCTGACCCG GCGTGTGCAG AAGGCAGCTC GTAGACAGGC CAAGCAAGAG 
GAGCTGAAGC GGCTGCACCG AGCACAGATC ATCCAGAGGC AGCTGGAGCA GGTGGAGGAG 
AAGCAGAGAC AGCTGGAGGA AAGAGGGGTG GCCGTGGAGA AGGCGCTCCG TGGTGAAGCA 
GACTATTGGG GAGAGTCTTA TTACAGTGAC CTCATCGACT TGCATCTGGG TGTTGAGCCC 
AGTGGCGGGA CTCCACGGCG TAGACCTCTC TCCTTCTGCC CTTGCTGTGT CCAGGAAGGC 
ATGGGCAAGA AGGATGACCC CAAGCTGATG CAGGAGTGGT TCAAGTTGGT GCAAGAGAAG 
AATGCCATGG TGCGCTATGA GTCGGAGCTG ATGATTTTTG CCCGGGAGCT GGAACTGGAG 
GACCGGCAGA GCCGACTGCA GCAGGAGCTC CGGGAGCGGA TGGCGGTGGA AGATCACCTG 
AAGACTGAGG AGGAGCTGTC TGAGGAGAAG AAGATCTTGA ACGAGATGCT GGAGGTGGTG 
GAGCAGAGAG ATGCCCTCGT GGCCCTGCTT GAGGAGCAGC GACTCCGGGA GAAGGAGGAG 
GACAAAGACC TGGAGGCTGT CATGCTGTCC AAGGGCTTCA GCCTGAACTG GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_022410878.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022266586.1 Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X9, mRNA.

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CloneID	OCe282674
Clone ID Related Accession (Same CDS sequence)	XM_022410878.1
Accession Version	XM_022410878.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	F-actin-methionine sulfoxide oxidase MICAL3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTGCCTGAC CTTCCCTCCA TACGCCGCGC AGCAGTGCAG 
GCCTGGCTGG AGACGGTCTC TGGAGTCCCA TTTGATGAGA ATGACCTGGA GGAAGACGTG 
GACTCGGAGC CAGCAAAGAT AGAAGGGGAG GCCACAGAGA ATGGGGACAC AGGGGACACT 
GGTGCTGAGC TGGACGATGA TCAGCGCTGG TCTGACGGGA TCCCATCGGA TGCCGAGACG 
GATCTCCGCG TGCAGCACCA GGAGGCGGTA GAGGCAGACC TGGAGCTGAG GGTCTCAGAG 
AATGAGGAGG AGCCACCTGA GGTCACACCA GGGCATCCGG AGAGAAGTCT CACCCGGGTC 
CCCAGCCCTG CCCTGTCCCC TGAGGAGCAA GCTGGTCTGC GGTCCCCAGG CCACAGCCCT 
AAGGCAGAGG GGGCCCAAAT CCCACTTGTC TCTGTTGTCA CCAAGGCAAA ATTGCCTGAA 
GAGAACCTTT TCCCCAAGCC TTTGCTCCCC AAAGTGAAGC CCAAGGTGGA AGTGCCCTGC 
AGTCAGAGAG CTGCATGCTC CCCCATCCGT TCTCAGCCAG TGGCTCTGCC AGAAGCCAGG 
ACACCTACCT CCCCAGTGAG CTCGCAGTGC CTGTCGCCTT TGGCCACCTC CACCCCCACC 
AGCACCCAGC TCCCCATTTG CTCCCAGCCT CAGCCTTCCT CTGAGACCAC GGCCCCATCC 
CCCACCAAGT CCCCCATACG GCTCCAGCCT GTTTCAGCCA AAACATCTAT CCCAGTGGTT 
CCCCTCCCCT TGAAGAACCA AGGGGACACC AAGGACAGGC TGGGCAGCCC TCTCGCTGTG 
GATGAGGCCC TGAAGCGGAA TGACCTGGTG GCAGAGTTCT GGATGAAGAG CGCCGAGATC 
CGCCGCAGCC TCGGGCTCAC ACCAGTGGAT CGGAGCAAGG TGTATGAGCC CAGCTTCCCT 
TCGCCTGCCT TTAAACCAGT GTCCCTAAAA TCCTATGCAG TGGAGAAGTC TCCCGAGGGG 
GAGGGACTCC AGCTTCTGAA GCCTCCACCT GTTCCTAAGA GACTAGGCCT GCCGAAGTCA 
GACGGTGACC AGCCCTTCCT GCCAACCCCC AAGTCCCCGT CGGACAGAGA GCTGAAAAGC 
TCCCAGGAGG AGAGGAGAGA TCTGTCGAGC AGCTCCGGGC TGGGCCTTCA CGGGAGCTCC 
TCCAACATGA AGACTTTGGG CAGCCAGAGC TTCAACACAT CAGACTCCAC CATGCTCACT 
CCCCCTTCAA GCCCACCGCC ACCACCACCC CAAGACGAGG AACCCGCAAC TCTTCGGAGG 
AAGCCATATC AGACTCATGA GCATAAGGAC GCTGAGCCCA AGGCCTCGGT GATTCCACCA 
CCACCACCTG CGACGTTCAT GCGGGCCCCC CGGGAGCCCA CCCAGCCCCT CCAAGAGGAG 
ATGCGGAAGT CGTTTGTGGA GAGCGTGGAT GAGATCCCAT TTGCCGATGA TGTGGAGGAC 
ACCTATGATG ACAAGACCGA GGACTCGAGT CTCCAGGAGA AGTTCTTCAC ACCCCCCTCC 
TACTGGCCCC GCACGGAGAG GCCCCTCCAC CCACCCCTAG CCAAGGAGAA TGGTAGGTTG 
CCTACTCTGG AGAGTGGGGT CCAGCCACAG AAGAGGGGCC TGCCCTTAGT CTCTCCAGAA 
GCCAAGGAGC TGGCTGAGGA GCGCATGCGA GCCAGGGAGA AGTCTGTGAA GAGCCAGGCG 
TTGAGAGACG CTATGGCCAA GCAGCTGAGT AAGATGAAGG AAATGGAGAT GGCGGCTGGG 
ACCTCCAGTC CCCCAGGAGG CACCTCCCAC AAAGCCTCCT CTGTTCCCTC CAAGGGCAAA 
GACCTCAGCC CCGAGTCCCC TAGACGCCCA GTTCTCAAAG GCCCCAGGGA GCCTGCCCTG 
AAGCACGAAG CCACTAGTGA GGAGGTCCTC TCCCCACCAT CGGACTCAGG GGGCCCAGAT 
GGTTCGGTCA CCTCATCCGA GGGCTCCAGT GGGAAGAGCA AGAAGAGATC GTCACTCTTC 
TCCCCCCGCA GAAACAAGAA AGAGAAGAAG TCCAAAGGTG AGGGCCGGCC CCCGGAGAAG 
CCCAGCCCAA GCCTTCTGGA GGAAGCTGCT GCCAAGCCCA AGTCCCTGTG GAAGTCGGTC 
TTTTCCGGGT ACAAGAAGGA CAAAAAGAAG AAGGGTGATG ACAAGTCCTG TTCCAGCACC 
CCTTCCAGCG GTGCCACTGT GGACTCTGGC AAGCACAAGA TGTCCCCTGT TGTGAGAGCA 
GAGCTGCACC TGCGGCGCCA GTTGAGCTTC TCCGAGGACT CAGACCTGTC CAGTGATGAC 
ATCCTTGAGA GGTCCTCCCA GAAGTCCAGG CGAGAGTCGG TTTATGTACC ACACGCCTTG 
GCATTCAAGA GATCATATCC ATCAAAGCCG AGAACCTACA CAGAGGAGGA ACTGAATGCC 
AAGCTGACCC GGCGTGTGCA GAAGGCAGCT CGTAGACAGG CCAAGCAAGA GGAGCTGAAG 
CGGCTGCACC GAGCACAGAT CATCCAGAGG CAGCTGGAGC AGGTGGAGGA GAAGCAGAGA 
CAGCTGGAGG AAAGAGGGGT GGCCGTGGAG AAGGCGCTCC GTGGTGAAGC AGTTGAGCCC 
AGTGGCGGGA CTCCACGGCG TAGACCTCTC TCCTTCTGCC CTTGCTGTGT CCAGGAAGGC 
ATGGGCAAGA AGGATGACCC CAAGCTGATG CAGGAGTGGT TCAAGTTGGT GCAAGAGAAG 
AATGCCATGG TGCGCTATGA GTCGGAGCTG ATGATTTTTG CCCGGGAGCT GGAACTGGAG 
GACCGGCAGA GCCGACTGCA GCAGGAGCTC CGGGAGCGGA TGGCGGTGGA AGATCACCTG 
AAGACTGAGG AGGAGCTGTC TGAGGAGAAG AAGATCTTGA ACGAGATGCT GGAGGTGGTG 
GAGCAGAGAG ATGCCCTCGT GGCCCTGCTT GAGGAGCAGC GACTCCGGGA GAAGGAGGAG 
GACAAAGACC TGGAGGCTGT CATGCTGTCC AAGGGCTTCA GCCTGAACTG GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022266586.1
CDS	258..7034
Translation	MEDSKNETVNHAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDYRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKACTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFQGLVQPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELKEATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLNYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREQNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNININFLRPSQVRHLYNTGETRDIHLEMENLVNSRTTPKLTRNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGKEMASVGEPDKLSMVMYLTQFYEMFKDSLPSSDALDLNAEEKAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEEAPRGYRGGRPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSTGMRRQPTQERGVSQPSCCLPEQGHPAPTPQWKQQQEKERSRTCPKKVITLSPPLTPPPCRAPRSQQTYRDLDADSRGKQSPHHERPEPEPSRRFFVDQWELSLSLRSSNRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCRKRVYVMERLSAEGKFFHRSCFKCEYCATTLRLSAYAYDIEDGKFYCKPHYCYRLSGSAQRKRPAVAPLSGKEAREPLQDGPTVDTNGRASAVTSPAERTPGSSVNGVEEPSIAKRLRGTPERIELENYRLSVKQAEELEEVPEETQAEHNLSSVLDTGAEEDVASSSSESEMEEEEEEEPPLPTSDLGGVPWKEAVRIHALLKGKSEEELEASKSFGPGHEEDEEEEEYEEEEEEEEEEYEEEEEEEESSEEGEYCPWDRELLQGQWLRQLSQEETTGTRKVGSQRLQQIVNPADPLEIQADVHWTHIREREEEERMVPTSESSTSRVPDLPSIRRAAVQAWLETVSGVPFDENDLEEDVDSEPAKIEGEATENGDTGDTGAELDDDQRWSDGIPSDAETDLRVQHQEAVEADLELRVSENEEEPPEVTPGHPERSLTRVPSPALSPEEQAGLRSPGHSPKAEGAQIPLVSVVTKAKLPEENLFPKPLLPKVKPKVEVPCSQRAACSPIRSQPVALPEARTPTSPVSSQCLSPLATSTPTSTQLPICSQPQPSSETTAPSPTKSPIRLQPVSAKTSIPVVPLPLKNQGDTKDRLGSPLAVDEALKRNDLVAEFWMKSAEIRRSLGLTPVDRSKVYEPSFPSPAFKPVSLKSYAVEKSPEGEGLQLLKPPPVPKRLGLPKSDGDQPFLPTPKSPSDRELKSSQEERRDLSSSSGLGLHGSSSNMKTLGSQSFNTSDSTMLTPPSSPPPPPPQDEEPATLRRKPYQTHEHKDAEPKASVIPPPPPATFMRAPREPTQPLQEEMRKSFVESVDEIPFADDVEDTYDDKTEDSSLQEKFFTPPSYWPRTERPLHPPLAKENGRLPTLESGVQPQKRGLPLVSPEAKELAEERMRAREKSVKSQALRDAMAKQLSKMKEMEMAAGTSSPPGGTSHKASSVPSKGKDLSPESPRRPVLKGPREPALKHEATSEEVLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRNKKEKKSKGEGRPPEKPSPSLLEEAAAKPKSLWKSVFSGYKKDKKKKGDDKSCSSTPSSGATVDSGKHKMSPVVRAELHLRRQLSFSEDSDLSSDDILERSSQKSRRESVYVPHALAFKRSYPSKPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELSEEKKILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAVMLSKGFSLNWS

Target ORF information:

RefSeq Version	XM_022410878.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022410878.1

ORF Insert Sequence:

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTGCCTGAC CTTCCCTCCA TACGCCGCGC AGCAGTGCAG 
GCCTGGCTGG AGACGGTCTC TGGAGTCCCA TTTGATGAGA ATGACCTGGA GGAAGACGTG 
GACTCGGAGC CAGCAAAGAT AGAAGGGGAG GCCACAGAGA ATGGGGACAC AGGGGACACT 
GGTGCTGAGC TGGACGATGA TCAGCGCTGG TCTGACGGGA TCCCATCGGA TGCCGAGACG 
GATCTCCGCG TGCAGCACCA GGAGGCGGTA GAGGCAGACC TGGAGCTGAG GGTCTCAGAG 
AATGAGGAGG AGCCACCTGA GGTCACACCA GGGCATCCGG AGAGAAGTCT CACCCGGGTC 
CCCAGCCCTG CCCTGTCCCC TGAGGAGCAA GCTGGTCTGC GGTCCCCAGG CCACAGCCCT 
AAGGCAGAGG GGGCCCAAAT CCCACTTGTC TCTGTTGTCA CCAAGGCAAA ATTGCCTGAA 
GAGAACCTTT TCCCCAAGCC TTTGCTCCCC AAAGTGAAGC CCAAGGTGGA AGTGCCCTGC 
AGTCAGAGAG CTGCATGCTC CCCCATCCGT TCTCAGCCAG TGGCTCTGCC AGAAGCCAGG 
ACACCTACCT CCCCAGTGAG CTCGCAGTGC CTGTCGCCTT TGGCCACCTC CACCCCCACC 
AGCACCCAGC TCCCCATTTG CTCCCAGCCT CAGCCTTCCT CTGAGACCAC GGCCCCATCC 
CCCACCAAGT CCCCCATACG GCTCCAGCCT GTTTCAGCCA AAACATCTAT CCCAGTGGTT 
CCCCTCCCCT TGAAGAACCA AGGGGACACC AAGGACAGGC TGGGCAGCCC TCTCGCTGTG 
GATGAGGCCC TGAAGCGGAA TGACCTGGTG GCAGAGTTCT GGATGAAGAG CGCCGAGATC 
CGCCGCAGCC TCGGGCTCAC ACCAGTGGAT CGGAGCAAGG TGTATGAGCC CAGCTTCCCT 
TCGCCTGCCT TTAAACCAGT GTCCCTAAAA TCCTATGCAG TGGAGAAGTC TCCCGAGGGG 
GAGGGACTCC AGCTTCTGAA GCCTCCACCT GTTCCTAAGA GACTAGGCCT GCCGAAGTCA 
GACGGTGACC AGCCCTTCCT GCCAACCCCC AAGTCCCCGT CGGACAGAGA GCTGAAAAGC 
TCCCAGGAGG AGAGGAGAGA TCTGTCGAGC AGCTCCGGGC TGGGCCTTCA CGGGAGCTCC 
TCCAACATGA AGACTTTGGG CAGCCAGAGC TTCAACACAT CAGACTCCAC CATGCTCACT 
CCCCCTTCAA GCCCACCGCC ACCACCACCC CAAGACGAGG AACCCGCAAC TCTTCGGAGG 
AAGCCATATC AGACTCATGA GCATAAGGAC GCTGAGCCCA AGGCCTCGGT GATTCCACCA 
CCACCACCTG CGACGTTCAT GCGGGCCCCC CGGGAGCCCA CCCAGCCCCT CCAAGAGGAG 
ATGCGGAAGT CGTTTGTGGA GAGCGTGGAT GAGATCCCAT TTGCCGATGA TGTGGAGGAC 
ACCTATGATG ACAAGACCGA GGACTCGAGT CTCCAGGAGA AGTTCTTCAC ACCCCCCTCC 
TACTGGCCCC GCACGGAGAG GCCCCTCCAC CCACCCCTAG CCAAGGAGAA TGGTAGGTTG 
CCTACTCTGG AGAGTGGGGT CCAGCCACAG AAGAGGGGCC TGCCCTTAGT CTCTCCAGAA 
GCCAAGGAGC TGGCTGAGGA GCGCATGCGA GCCAGGGAGA AGTCTGTGAA GAGCCAGGCG 
TTGAGAGACG CTATGGCCAA GCAGCTGAGT AAGATGAAGG AAATGGAGAT GGCGGCTGGG 
ACCTCCAGTC CCCCAGGAGG CACCTCCCAC AAAGCCTCCT CTGTTCCCTC CAAGGGCAAA 
GACCTCAGCC CCGAGTCCCC TAGACGCCCA GTTCTCAAAG GCCCCAGGGA GCCTGCCCTG 
AAGCACGAAG CCACTAGTGA GGAGGTCCTC TCCCCACCAT CGGACTCAGG GGGCCCAGAT 
GGTTCGGTCA CCTCATCCGA GGGCTCCAGT GGGAAGAGCA AGAAGAGATC GTCACTCTTC 
TCCCCCCGCA GAAACAAGAA AGAGAAGAAG TCCAAAGGTG AGGGCCGGCC CCCGGAGAAG 
CCCAGCCCAA GCCTTCTGGA GGAAGCTGCT GCCAAGCCCA AGTCCCTGTG GAAGTCGGTC 
TTTTCCGGGT ACAAGAAGGA CAAAAAGAAG AAGGGTGATG ACAAGTCCTG TTCCAGCACC 
CCTTCCAGCG GTGCCACTGT GGACTCTGGC AAGCACAAGA TGTCCCCTGT TGTGAGAGCA 
GAGCTGCACC TGCGGCGCCA GTTGAGCTTC TCCGAGGACT CAGACCTGTC CAGTGATGAC 
ATCCTTGAGA GGTCCTCCCA GAAGTCCAGG CGAGAGTCGG TTTATGTACC ACACGCCTTG 
GCATTCAAGA GATCATATCC ATCAAAGCCG AGAACCTACA CAGAGGAGGA ACTGAATGCC 
AAGCTGACCC GGCGTGTGCA GAAGGCAGCT CGTAGACAGG CCAAGCAAGA GGAGCTGAAG 
CGGCTGCACC GAGCACAGAT CATCCAGAGG CAGCTGGAGC AGGTGGAGGA GAAGCAGAGA 
CAGCTGGAGG AAAGAGGGGT GGCCGTGGAG AAGGCGCTCC GTGGTGAAGC AGTTGAGCCC 
AGTGGCGGGA CTCCACGGCG TAGACCTCTC TCCTTCTGCC CTTGCTGTGT CCAGGAAGGC 
ATGGGCAAGA AGGATGACCC CAAGCTGATG CAGGAGTGGT TCAAGTTGGT GCAAGAGAAG 
AATGCCATGG TGCGCTATGA GTCGGAGCTG ATGATTTTTG CCCGGGAGCT GGAACTGGAG 
GACCGGCAGA GCCGACTGCA GCAGGAGCTC CGGGAGCGGA TGGCGGTGGA AGATCACCTG 
AAGACTGAGG AGGAGCTGTC TGAGGAGAAG AAGATCTTGA ACGAGATGCT GGAGGTGGTG 
GAGCAGAGAG ATGCCCTCGT GGCCCTGCTT GAGGAGCAGC GACTCCGGGA GAAGGAGGAG 
GACAAAGACC TGGAGGCTGT CATGCTGTCC AAGGGCTTCA GCCTGAACTG GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_022410879.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022266587.1 Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X10, mRNA.

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CloneID	OCe282675
Clone ID Related Accession (Same CDS sequence)	XM_022410879.1
Accession Version	XM_022410879.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	F-actin-methionine sulfoxide oxidase MICAL3 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTCCCATTT GATGAGAATG ACCTGGAGGA AGACGTGGAC 
TCGGAGCCAG CAAAGATAGA AGGGGAGGCC ACAGAGAATG GGGACACAGG GGACACTGGT 
GCTGAGCTGG ACGATGATCA GCGCTGGTCT GACGGGATCC CATCGGATGC CGAGACGGAT 
CTCCGCGTGC AGCACCAGGA GGCGGTAGAG GCAGACCTGG AGCTGAGGGT CTCAGAGAAT 
GAGGAGGAGC CACCTGAGGT CACACCAGGG CATCCGGAGA GAAGTCTCAC CCGGGTCCCC 
AGCCCTGCCC TGTCCCCTGA GGAGCAAGCT GGTCTGCGGT CCCCAGGCCA CAGCCCTAAG 
GCAGAGGGGG CCCAAATCCC ACTTGTCTCT GTTGTCACCA AGGCAAAATT GCCTGAAGAG 
AACCTTTTCC CCAAGCCTTT GCTCCCCAAA GTGAAGCCCA AGGTGGAAGT GCCCTGCAGT 
CAGAGAGCTG CATGCTCCCC CATCCGTTCT CAGCCAGTGG CTCTGCCAGA AGCCAGGACA 
CCTACCTCCC CAGTGAGCTC GCAGTGCCTG TCGCCTTTGG CCACCTCCAC CCCCACCAGC 
ACCCAGCTCC CCATTTGCTC CCAGCCTCAG CCTTCCTCTG AGACCACGGC CCCATCCCCC 
ACCAAGTCCC CCATACGGCT CCAGCCTGTT TCAGCCAAAA CATCTATCCC AGTGGTTCCC 
CTCCCCTTGA AGAACCAAGG GGACACCAAG GACAGGCTGG GCAGCCCTCT CGCTGTGGAT 
GAGGCCCTGA AGCGGAATGA CCTGGTGGCA GAGTTCTGGA TGAAGAGCGC CGAGATCCGC 
CGCAGCCTCG GGCTCACACC AGTGGATCGG AGCAAGGTGT ATGAGCCCAG CTTCCCTTCG 
CCTGCCTTTA AACCAGTGTC CCTAAAATCC TATGCAGTGG AGAAGTCTCC CGAGGGGGAG 
GGACTCCAGC TTCTGAAGCC TCCACCTGTT CCTAAGAGAC TAGGCCTGCC GAAGTCAGAC 
GGTGACCAGC CCTTCCTGCC AACCCCCAAG TCCCCGTCGG ACAGAGAGCT GAAAAGCTCC 
CAGGAGGAGA GGAGAGATCT GTCGAGCAGC TCCGGGCTGG GCCTTCACGG GAGCTCCTCC 
AACATGAAGA CTTTGGGCAG CCAGAGCTTC AACACATCAG ACTCCACCAT GCTCACTCCC 
CCTTCAAGCC CACCGCCACC ACCACCCCAA GACGAGGAAC CCGCAACTCT TCGGAGGAAG 
CCATATCAGA CTCATGAGCA TAAGGACGCT GAGCCCAAGG CCTCGGTGAT TCCACCACCA 
CCACCTGCGA CGTTCATGCG GGCCCCCCGG GAGCCCACCC AGCCCCTCCA AGAGGAGATG 
CGGAAGTCGT TTGTGGAGAG CGTGGATGAG ATCCCATTTG CCGATGATGT GGAGGACACC 
TATGATGACA AGACCGAGGA CTCGAGTCTC CAGGAGAAGT TCTTCACACC CCCCTCCTAC 
TGGCCCCGCA CGGAGAGGCC CCTCCACCCA CCCCTAGCCA AGGAGAATGG TAGGTTGCCT 
ACTCTGGAGA GTGGGGTCCA GCCACAGAAG AGGGGCCTGC CCTTAGTCTC TCCAGAAGCC 
AAGGAGCTGG CTGAGGAGCG CATGCGAGCC AGGGAGAAGT CTGTGAAGAG CCAGGCGTTG 
AGAGACGCTA TGGCCAAGCA GCTGAGTAAG ATGAAGGAAA TGGAGATGGC GGCTGGGACC 
TCCAGTCCCC CAGGAGGCAC CTCCCACAAA GCCTCCTCTG TTCCCTCCAA GGGCAAAGAC 
CTCAGCCCCG AGTCCCCTAG ACGCCCAGTT CTCAAAGGCC CCAGGGAGCC TGCCCTGAAG 
CACGAAGCCA CTAGTGAGGA GGTCCTCTCC CCACCATCGG ACTCAGGGGG CCCAGATGGT 
TCGGTCACCT CATCCGAGGG CTCCAGTGGG AAGAGCAAGA AGAGATCGTC ACTCTTCTCC 
CCCCGCAGAA ACAAGAAAGA GAAGAAGTCC AAAGGTGAGG GCCGGCCCCC GGAGAAGCCC 
AGCCCAAGCC TTCTGGAGGA AGCTGCTGCC AAGCCCAAGT CCCTGTGGAA GTCGGTCTTT 
TCCGGGTACA AGAAGGACAA AAAGAAGAAG GGTGATGACA AGTCCTGTTC CAGCACCCCT 
TCCAGCGGTG CCACTGTGGA CTCTGGCAAG CACAAGATGT CCCCTGTTGT GAGAGCAGAG 
CTGCACCTGC GGCGCCAGTT GAGCTTCTCC GAGGACTCAG ACCTGTCCAG TGATGACATC 
CTTGAGAGGT CCTCCCAGAA GTCCAGGCGA GAGTCGGTTT ATGTACCACA CGCCTTGGCA 
TTCAAGAGAT CATATCCATC AAAGCCGAGA ACCTACACAG AGGAGGAACT GAATGCCAAG 
CTGACCCGGC GTGTGCAGAA GGCAGCTCGT AGACAGGCCA AGCAAGAGGA GCTGAAGCGG 
CTGCACCGAG CACAGATCAT CCAGAGGCAG CTGGAGCAGG TGGAGGAGAA GCAGAGACAG 
CTGGAGGAAA GAGGGGTGGC CGTGGAGAAG GCGCTCCGTG GTGAAGCAGA CTATTGGGGA 
GAGTCTTATT ACAGTGACCT CATCGACTTG CATCTGGGTG TTGAGCCCAG TGGCGGGACT 
CCACGGCGTA GACCTCTCTC CTTCTGCCCT TGCTGTGTCC AGGAAGGCAT GGGCAAGAAG 
GATGACCCCA AGCTGATGCA GGAGTGGTTC AAGTTGGTGC AAGAGAAGAA TGCCATGGTG 
CGCTATGAGT CGGAGCTGAT GATTTTTGCC CGGGAGCTGG AACTGGAGGA CCGGCAGAGC 
CGACTGCAGC AGGAGCTCCG GGAGCGGATG GCGGTGGAAG ATCACCTGAA GACTGAGGAG 
GAGCTGTCTG AGGAGAAGAA GATCTTGAAC GAGATGCTGG AGGTGGTGGA GCAGAGAGAT 
GCCCTCGTGG CCCTGCTTGA GGAGCAGCGA CTCCGGGAGA AGGAGGAGGA CAAAGACCTG 
GAGGCTGTCA TGCTGTCCAA GGGCTTCAGC CTGAACTGGT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022266587.1
CDS	258..7022
Translation	MEDSKNETVNHAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDYRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKACTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFQGLVQPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELKEATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLNYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREQNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNININFLRPSQVRHLYNTGETRDIHLEMENLVNSRTTPKLTRNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGKEMASVGEPDKLSMVMYLTQFYEMFKDSLPSSDALDLNAEEKAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEEAPRGYRGGRPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSTGMRRQPTQERGVSQPSCCLPEQGHPAPTPQWKQQQEKERSRTCPKKVITLSPPLTPPPCRAPRSQQTYRDLDADSRGKQSPHHERPEPEPSRRFFVDQWELSLSLRSSNRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCRKRVYVMERLSAEGKFFHRSCFKCEYCATTLRLSAYAYDIEDGKFYCKPHYCYRLSGSAQRKRPAVAPLSGKEAREPLQDGPTVDTNGRASAVTSPAERTPGSSVNGVEEPSIAKRLRGTPERIELENYRLSVKQAEELEEVPEETQAEHNLSSVLDTGAEEDVASSSSESEMEEEEEEEPPLPTSDLGGVPWKEAVRIHALLKGKSEEELEASKSFGPGHEEDEEEEEYEEEEEEEEEEYEEEEEEEESSEEGEYCPWDRELLQGQWLRQLSQEETTGTRKVGSQRLQQIVNPADPLEIQADVHWTHIREREEEERMVPTSESSTSRVPFDENDLEEDVDSEPAKIEGEATENGDTGDTGAELDDDQRWSDGIPSDAETDLRVQHQEAVEADLELRVSENEEEPPEVTPGHPERSLTRVPSPALSPEEQAGLRSPGHSPKAEGAQIPLVSVVTKAKLPEENLFPKPLLPKVKPKVEVPCSQRAACSPIRSQPVALPEARTPTSPVSSQCLSPLATSTPTSTQLPICSQPQPSSETTAPSPTKSPIRLQPVSAKTSIPVVPLPLKNQGDTKDRLGSPLAVDEALKRNDLVAEFWMKSAEIRRSLGLTPVDRSKVYEPSFPSPAFKPVSLKSYAVEKSPEGEGLQLLKPPPVPKRLGLPKSDGDQPFLPTPKSPSDRELKSSQEERRDLSSSSGLGLHGSSSNMKTLGSQSFNTSDSTMLTPPSSPPPPPPQDEEPATLRRKPYQTHEHKDAEPKASVIPPPPPATFMRAPREPTQPLQEEMRKSFVESVDEIPFADDVEDTYDDKTEDSSLQEKFFTPPSYWPRTERPLHPPLAKENGRLPTLESGVQPQKRGLPLVSPEAKELAEERMRAREKSVKSQALRDAMAKQLSKMKEMEMAAGTSSPPGGTSHKASSVPSKGKDLSPESPRRPVLKGPREPALKHEATSEEVLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRNKKEKKSKGEGRPPEKPSPSLLEEAAAKPKSLWKSVFSGYKKDKKKKGDDKSCSSTPSSGATVDSGKHKMSPVVRAELHLRRQLSFSEDSDLSSDDILERSSQKSRRESVYVPHALAFKRSYPSKPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELSEEKKILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAVMLSKGFSLNWS

Target ORF information:

RefSeq Version	XM_022410879.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022410879.1

ORF Insert Sequence:

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTCCCATTT GATGAGAATG ACCTGGAGGA AGACGTGGAC 
TCGGAGCCAG CAAAGATAGA AGGGGAGGCC ACAGAGAATG GGGACACAGG GGACACTGGT 
GCTGAGCTGG ACGATGATCA GCGCTGGTCT GACGGGATCC CATCGGATGC CGAGACGGAT 
CTCCGCGTGC AGCACCAGGA GGCGGTAGAG GCAGACCTGG AGCTGAGGGT CTCAGAGAAT 
GAGGAGGAGC CACCTGAGGT CACACCAGGG CATCCGGAGA GAAGTCTCAC CCGGGTCCCC 
AGCCCTGCCC TGTCCCCTGA GGAGCAAGCT GGTCTGCGGT CCCCAGGCCA CAGCCCTAAG 
GCAGAGGGGG CCCAAATCCC ACTTGTCTCT GTTGTCACCA AGGCAAAATT GCCTGAAGAG 
AACCTTTTCC CCAAGCCTTT GCTCCCCAAA GTGAAGCCCA AGGTGGAAGT GCCCTGCAGT 
CAGAGAGCTG CATGCTCCCC CATCCGTTCT CAGCCAGTGG CTCTGCCAGA AGCCAGGACA 
CCTACCTCCC CAGTGAGCTC GCAGTGCCTG TCGCCTTTGG CCACCTCCAC CCCCACCAGC 
ACCCAGCTCC CCATTTGCTC CCAGCCTCAG CCTTCCTCTG AGACCACGGC CCCATCCCCC 
ACCAAGTCCC CCATACGGCT CCAGCCTGTT TCAGCCAAAA CATCTATCCC AGTGGTTCCC 
CTCCCCTTGA AGAACCAAGG GGACACCAAG GACAGGCTGG GCAGCCCTCT CGCTGTGGAT 
GAGGCCCTGA AGCGGAATGA CCTGGTGGCA GAGTTCTGGA TGAAGAGCGC CGAGATCCGC 
CGCAGCCTCG GGCTCACACC AGTGGATCGG AGCAAGGTGT ATGAGCCCAG CTTCCCTTCG 
CCTGCCTTTA AACCAGTGTC CCTAAAATCC TATGCAGTGG AGAAGTCTCC CGAGGGGGAG 
GGACTCCAGC TTCTGAAGCC TCCACCTGTT CCTAAGAGAC TAGGCCTGCC GAAGTCAGAC 
GGTGACCAGC CCTTCCTGCC AACCCCCAAG TCCCCGTCGG ACAGAGAGCT GAAAAGCTCC 
CAGGAGGAGA GGAGAGATCT GTCGAGCAGC TCCGGGCTGG GCCTTCACGG GAGCTCCTCC 
AACATGAAGA CTTTGGGCAG CCAGAGCTTC AACACATCAG ACTCCACCAT GCTCACTCCC 
CCTTCAAGCC CACCGCCACC ACCACCCCAA GACGAGGAAC CCGCAACTCT TCGGAGGAAG 
CCATATCAGA CTCATGAGCA TAAGGACGCT GAGCCCAAGG CCTCGGTGAT TCCACCACCA 
CCACCTGCGA CGTTCATGCG GGCCCCCCGG GAGCCCACCC AGCCCCTCCA AGAGGAGATG 
CGGAAGTCGT TTGTGGAGAG CGTGGATGAG ATCCCATTTG CCGATGATGT GGAGGACACC 
TATGATGACA AGACCGAGGA CTCGAGTCTC CAGGAGAAGT TCTTCACACC CCCCTCCTAC 
TGGCCCCGCA CGGAGAGGCC CCTCCACCCA CCCCTAGCCA AGGAGAATGG TAGGTTGCCT 
ACTCTGGAGA GTGGGGTCCA GCCACAGAAG AGGGGCCTGC CCTTAGTCTC TCCAGAAGCC 
AAGGAGCTGG CTGAGGAGCG CATGCGAGCC AGGGAGAAGT CTGTGAAGAG CCAGGCGTTG 
AGAGACGCTA TGGCCAAGCA GCTGAGTAAG ATGAAGGAAA TGGAGATGGC GGCTGGGACC 
TCCAGTCCCC CAGGAGGCAC CTCCCACAAA GCCTCCTCTG TTCCCTCCAA GGGCAAAGAC 
CTCAGCCCCG AGTCCCCTAG ACGCCCAGTT CTCAAAGGCC CCAGGGAGCC TGCCCTGAAG 
CACGAAGCCA CTAGTGAGGA GGTCCTCTCC CCACCATCGG ACTCAGGGGG CCCAGATGGT 
TCGGTCACCT CATCCGAGGG CTCCAGTGGG AAGAGCAAGA AGAGATCGTC ACTCTTCTCC 
CCCCGCAGAA ACAAGAAAGA GAAGAAGTCC AAAGGTGAGG GCCGGCCCCC GGAGAAGCCC 
AGCCCAAGCC TTCTGGAGGA AGCTGCTGCC AAGCCCAAGT CCCTGTGGAA GTCGGTCTTT 
TCCGGGTACA AGAAGGACAA AAAGAAGAAG GGTGATGACA AGTCCTGTTC CAGCACCCCT 
TCCAGCGGTG CCACTGTGGA CTCTGGCAAG CACAAGATGT CCCCTGTTGT GAGAGCAGAG 
CTGCACCTGC GGCGCCAGTT GAGCTTCTCC GAGGACTCAG ACCTGTCCAG TGATGACATC 
CTTGAGAGGT CCTCCCAGAA GTCCAGGCGA GAGTCGGTTT ATGTACCACA CGCCTTGGCA 
TTCAAGAGAT CATATCCATC AAAGCCGAGA ACCTACACAG AGGAGGAACT GAATGCCAAG 
CTGACCCGGC GTGTGCAGAA GGCAGCTCGT AGACAGGCCA AGCAAGAGGA GCTGAAGCGG 
CTGCACCGAG CACAGATCAT CCAGAGGCAG CTGGAGCAGG TGGAGGAGAA GCAGAGACAG 
CTGGAGGAAA GAGGGGTGGC CGTGGAGAAG GCGCTCCGTG GTGAAGCAGA CTATTGGGGA 
GAGTCTTATT ACAGTGACCT CATCGACTTG CATCTGGGTG TTGAGCCCAG TGGCGGGACT 
CCACGGCGTA GACCTCTCTC CTTCTGCCCT TGCTGTGTCC AGGAAGGCAT GGGCAAGAAG 
GATGACCCCA AGCTGATGCA GGAGTGGTTC AAGTTGGTGC AAGAGAAGAA TGCCATGGTG 
CGCTATGAGT CGGAGCTGAT GATTTTTGCC CGGGAGCTGG AACTGGAGGA CCGGCAGAGC 
CGACTGCAGC AGGAGCTCCG GGAGCGGATG GCGGTGGAAG ATCACCTGAA GACTGAGGAG 
GAGCTGTCTG AGGAGAAGAA GATCTTGAAC GAGATGCTGG AGGTGGTGGA GCAGAGAGAT 
GCCCTCGTGG CCCTGCTTGA GGAGCAGCGA CTCCGGGAGA AGGAGGAGGA CAAAGACCTG 
GAGGCTGTCA TGCTGTCCAA GGGCTTCAGC CTGAACTGGT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_022410880.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022266588.1 Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X11, mRNA.

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CloneID	OCe282676
Clone ID Related Accession (Same CDS sequence)	XM_022410880.1
Accession Version	XM_022410880.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	F-actin-methionine sulfoxide oxidase MICAL3 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTGCCTGAC CTTCCCTCCA TACGCCGCGC AGCAGTGCAG 
GCCTGGCTGG AGACGGTCTC TGGAGTCCCA TTTGATGAGA ATGACCTGGA GGAAGACGTG 
GACTCGGAGC CAGCAAAGAT AGAAGGGGAG GCCACAGAGA ATGGGGACAC AGGGGACACT 
GGTGCTGAGC TGGACGATGA TCAGCGCTGG TCTGACGGGA TCCCATCGGA TGCCGAGACG 
GATCTCCGCG TGCAGCACCA GGAGGCGGTA GAGGCAGACC TGGAGCTGAG GGTCTCAGAG 
AATGAGGAGG AGCCACCTGA GGTCACACCA GGGCATCCGG AGAGAAGTCT CACCCGGGTC 
CCCAGCCCTG CCCTGTCCCC TGAGGAGCAA GCTGGTCTGC GGTCCCCAGG CCACAGCCCT 
AAGGCAGAGG GGGCCCAAAT CCCACTTGTC TCTGTTGTCA CCAAGGCAAA ATTGCCTGAA 
GAGAACCTTT TCCCCAAGCC TTTGCTCCCC AAAGTGAAGC CCAAGGTGGA AGTGCCCTGC 
AGTCAGAGAG CTGCATGCTC CCCCATCCGT TCTCAGCCAG TGGCTCTGCC AGAAGCCAGG 
ACACCTACCT CCCCAGTGAG CTCGCAGTGC CTGTCGCCTT TGGCCACCTC CACCCCCACC 
AGCACCCAGC TCCCCATTTG CTCCCAGCCT CAGCCTTCCT CTGAGACCAC GGCCCCATCC 
CCCACCAAGT CCCCCATACG GCTCCAGCCT GTTTCAGCCA AAACATCTAT CCCAGTGGTT 
CCCCTCCCCT TGAAGAACCA AGGGGACACC AAGGACAGGC TGGGCAGCCC TCTCGCTGTG 
GATGAGGCCC TGAAGCGGAA TGACCTGGTG GCAGAGTTCT GGATGAAGAG CGCCGAGATC 
CGCCGCAGCC TCGGGCTCAC ACCAGTGGAT CGGAGCAAGG TGTATGAGCC CAGCTTCCCT 
TCGCCTGCCT TTAAACCAGT GTCCCTAAAA TCCTATGCAG TGGAGAAGTC TCCCGAGGGG 
GAGGGACTCC AGCTTCTGAA GCCTCCACCT GTTCCTAAGA GACTAGGCCT GCCGAAGTCA 
GACGGTGACC AGCCCTTCCT GCCAACCCCC AAGTCCCCGT CGGACAGAGA GCTGAAAAGC 
TCCCAGGAGG AGAGGAGAGA TCTGTCGAGC AGCTCCGGGC TGGGCCTTCA CGGGAGCTCC 
TCCAACATGA AGACTTTGGG CAGCCAGAGC TTCAACACAT CAGACTCCAC CATGCTCACT 
CCCCCTTCAA GCCCACCGCC ACCACCACCC CAAGACGAGG AACCCGCAAC TCTTCGGAGG 
AAGCCATATC AGACTCATGA GCATAAGGAC GCTGAGCCCA AGGCCTCGGT GATTCCACCA 
CCACCACCTG CGACGTTCAT GCGGGCCCCC CGGGAGCCCA CCCAGCCCCT CCAAGAGGAG 
ATGCGGAAGT CGTTTGTGGA GAGCGTGGAT GAGATCCCAT TTGCCGATGA TGTGGAGGAC 
ACCTATGATG ACAAGACCGA GGACTCGAGT CTCCAGGAGA AGTTCTTCAC ACCCCCCTCC 
TACTGGCCCC GCACGGAGAG GCCCCTCCAC CCACCCCTAG CCAAGGAGAA TGGTAGGTTG 
CCTACTCTGG AGAGTGGGGT CCAGCCACAG AAGAGGGGCC TGCCCTTAGT CTCTCCAGAA 
GCCAAGGAGC TGGCTGAGGA GCGCATGCGA GCCAGGGAGA AGTCTGTGAA GAGCCAGGCG 
TTGAGAGACG CTATGGCCAA GCAGCTGAGT AAGATGAAGG AAATGGAGAT GGCGGCTGGG 
ACCTCCAGTC CCCCAGGAGG CACCTCCCAC AAAGCCTCCT CTGTTCCCTC CAAGGGCAAA 
GACCTCAGCC CCGAGTCCCC TAGACGCCCA GTTCTCAAAG GCCCCAGGGA GCCTGCCCTG 
AAGCACGAAG CCACTAGTGA GGAGGTCCTC TCCCCACCAT CGGACTCAGG GGGCCCAGAT 
GGTTCGGTCA CCTCATCCGA GGGCTCCAGT GGGAAGAGCA AGAAGAGATC GTCACTCTTC 
TCCCCCCGCA GAAACAAGAA AGAGAAGAAG TCCAAAGGTG AGGGCCGGCC CCCGGAGAAG 
CCCAGCCCAA GCCTTCTGGA GGAAGCTGCT GCCAAGCCCA AGTCCCTGTG GAAGTCGGTC 
TTTTCCGGGT ACAAGAAGGA CAAAAAGAAG AAGGGTGATG ACAAGTCCTG TTCCAGCACC 
CCTTCCAGCG GTGCCACTGT GGACTCTGGC AAGCACAAGA TGTCCCCTGT TGTGAGAGCA 
GAGCTGCACC TGCGGCGCCA GTTGAGCTTC TCCGAGGACT CAGACCTGTC CAGTGATGAC 
ATCCTTGAGA GGTCCTCCCA GAAGTCCAGG CGAGAGTCGG TTTATGTACC ACACGCCTTG 
GCATTCAAGA GATCATATCC ATCAAAGCCG AGAACCTACA CAGAGGAGGA ACTGAATGCC 
AAGCTGACCC GGCGTGTGCA GAAGGCAGCT CGTAGACAGG CCAAGCAAGA GGAGCTGAAG 
CGGCTGCACC GAGCACAGAT CATCCAGAGG CAGCTGGAGC AGGTGGAGGA GAAGCAGAGA 
CAGCTGGAGG AAAGAGGGGT GGCCGTGGAG AAGGCGCTCC GTGGTGAAGC AGACTATTGG 
GGAGAGTCTT ATTACAGTGA CCTCATCGAC TTGCATCTGG GTGGCATGGG CAAGAAGGAT 
GACCCCAAGC TGATGCAGGA GTGGTTCAAG TTGGTGCAAG AGAAGAATGC CATGGTGCGC 
TATGAGTCGG AGCTGATGAT TTTTGCCCGG GAGCTGGAAC TGGAGGACCG GCAGAGCCGA 
CTGCAGCAGG AGCTCCGGGA GCGGATGGCG GTGGAAGATC ACCTGAAGAC TGAGGAGGAG 
CTGTCTGAGG AGAAGAAGAT CTTGAACGAG ATGCTGGAGG TGGTGGAGCA GAGAGATGCC 
CTCGTGGCCC TGCTTGAGGA GCAGCGACTC CGGGAGAAGG AGGAGGACAA AGACCTGGAG 
GCTGTCATGC TGTCCAAGGG CTTCAGCCTG AACTGGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022266588.1
CDS	258..7019
Translation	MEDSKNETVNHAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDYRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKACTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFQGLVQPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELKEATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLNYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREQNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNININFLRPSQVRHLYNTGETRDIHLEMENLVNSRTTPKLTRNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGKEMASVGEPDKLSMVMYLTQFYEMFKDSLPSSDALDLNAEEKAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEEAPRGYRGGRPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSTGMRRQPTQERGVSQPSCCLPEQGHPAPTPQWKQQQEKERSRTCPKKVITLSPPLTPPPCRAPRSQQTYRDLDADSRGKQSPHHERPEPEPSRRFFVDQWELSLSLRSSNRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCRKRVYVMERLSAEGKFFHRSCFKCEYCATTLRLSAYAYDIEDGKFYCKPHYCYRLSGSAQRKRPAVAPLSGKEAREPLQDGPTVDTNGRASAVTSPAERTPGSSVNGVEEPSIAKRLRGTPERIELENYRLSVKQAEELEEVPEETQAEHNLSSVLDTGAEEDVASSSSESEMEEEEEEEPPLPTSDLGGVPWKEAVRIHALLKGKSEEELEASKSFGPGHEEDEEEEEYEEEEEEEEEEYEEEEEEEESSEEGEYCPWDRELLQGQWLRQLSQEETTGTRKVGSQRLQQIVNPADPLEIQADVHWTHIREREEEERMVPTSESSTSRVPDLPSIRRAAVQAWLETVSGVPFDENDLEEDVDSEPAKIEGEATENGDTGDTGAELDDDQRWSDGIPSDAETDLRVQHQEAVEADLELRVSENEEEPPEVTPGHPERSLTRVPSPALSPEEQAGLRSPGHSPKAEGAQIPLVSVVTKAKLPEENLFPKPLLPKVKPKVEVPCSQRAACSPIRSQPVALPEARTPTSPVSSQCLSPLATSTPTSTQLPICSQPQPSSETTAPSPTKSPIRLQPVSAKTSIPVVPLPLKNQGDTKDRLGSPLAVDEALKRNDLVAEFWMKSAEIRRSLGLTPVDRSKVYEPSFPSPAFKPVSLKSYAVEKSPEGEGLQLLKPPPVPKRLGLPKSDGDQPFLPTPKSPSDRELKSSQEERRDLSSSSGLGLHGSSSNMKTLGSQSFNTSDSTMLTPPSSPPPPPPQDEEPATLRRKPYQTHEHKDAEPKASVIPPPPPATFMRAPREPTQPLQEEMRKSFVESVDEIPFADDVEDTYDDKTEDSSLQEKFFTPPSYWPRTERPLHPPLAKENGRLPTLESGVQPQKRGLPLVSPEAKELAEERMRAREKSVKSQALRDAMAKQLSKMKEMEMAAGTSSPPGGTSHKASSVPSKGKDLSPESPRRPVLKGPREPALKHEATSEEVLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRNKKEKKSKGEGRPPEKPSPSLLEEAAAKPKSLWKSVFSGYKKDKKKKGDDKSCSSTPSSGATVDSGKHKMSPVVRAELHLRRQLSFSEDSDLSSDDILERSSQKSRRESVYVPHALAFKRSYPSKPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELSEEKKILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAVMLSKGFSLNWS

Target ORF information:

RefSeq Version	XM_022410880.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022410880.1

ORF Insert Sequence:

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTGCCTGAC CTTCCCTCCA TACGCCGCGC AGCAGTGCAG 
GCCTGGCTGG AGACGGTCTC TGGAGTCCCA TTTGATGAGA ATGACCTGGA GGAAGACGTG 
GACTCGGAGC CAGCAAAGAT AGAAGGGGAG GCCACAGAGA ATGGGGACAC AGGGGACACT 
GGTGCTGAGC TGGACGATGA TCAGCGCTGG TCTGACGGGA TCCCATCGGA TGCCGAGACG 
GATCTCCGCG TGCAGCACCA GGAGGCGGTA GAGGCAGACC TGGAGCTGAG GGTCTCAGAG 
AATGAGGAGG AGCCACCTGA GGTCACACCA GGGCATCCGG AGAGAAGTCT CACCCGGGTC 
CCCAGCCCTG CCCTGTCCCC TGAGGAGCAA GCTGGTCTGC GGTCCCCAGG CCACAGCCCT 
AAGGCAGAGG GGGCCCAAAT CCCACTTGTC TCTGTTGTCA CCAAGGCAAA ATTGCCTGAA 
GAGAACCTTT TCCCCAAGCC TTTGCTCCCC AAAGTGAAGC CCAAGGTGGA AGTGCCCTGC 
AGTCAGAGAG CTGCATGCTC CCCCATCCGT TCTCAGCCAG TGGCTCTGCC AGAAGCCAGG 
ACACCTACCT CCCCAGTGAG CTCGCAGTGC CTGTCGCCTT TGGCCACCTC CACCCCCACC 
AGCACCCAGC TCCCCATTTG CTCCCAGCCT CAGCCTTCCT CTGAGACCAC GGCCCCATCC 
CCCACCAAGT CCCCCATACG GCTCCAGCCT GTTTCAGCCA AAACATCTAT CCCAGTGGTT 
CCCCTCCCCT TGAAGAACCA AGGGGACACC AAGGACAGGC TGGGCAGCCC TCTCGCTGTG 
GATGAGGCCC TGAAGCGGAA TGACCTGGTG GCAGAGTTCT GGATGAAGAG CGCCGAGATC 
CGCCGCAGCC TCGGGCTCAC ACCAGTGGAT CGGAGCAAGG TGTATGAGCC CAGCTTCCCT 
TCGCCTGCCT TTAAACCAGT GTCCCTAAAA TCCTATGCAG TGGAGAAGTC TCCCGAGGGG 
GAGGGACTCC AGCTTCTGAA GCCTCCACCT GTTCCTAAGA GACTAGGCCT GCCGAAGTCA 
GACGGTGACC AGCCCTTCCT GCCAACCCCC AAGTCCCCGT CGGACAGAGA GCTGAAAAGC 
TCCCAGGAGG AGAGGAGAGA TCTGTCGAGC AGCTCCGGGC TGGGCCTTCA CGGGAGCTCC 
TCCAACATGA AGACTTTGGG CAGCCAGAGC TTCAACACAT CAGACTCCAC CATGCTCACT 
CCCCCTTCAA GCCCACCGCC ACCACCACCC CAAGACGAGG AACCCGCAAC TCTTCGGAGG 
AAGCCATATC AGACTCATGA GCATAAGGAC GCTGAGCCCA AGGCCTCGGT GATTCCACCA 
CCACCACCTG CGACGTTCAT GCGGGCCCCC CGGGAGCCCA CCCAGCCCCT CCAAGAGGAG 
ATGCGGAAGT CGTTTGTGGA GAGCGTGGAT GAGATCCCAT TTGCCGATGA TGTGGAGGAC 
ACCTATGATG ACAAGACCGA GGACTCGAGT CTCCAGGAGA AGTTCTTCAC ACCCCCCTCC 
TACTGGCCCC GCACGGAGAG GCCCCTCCAC CCACCCCTAG CCAAGGAGAA TGGTAGGTTG 
CCTACTCTGG AGAGTGGGGT CCAGCCACAG AAGAGGGGCC TGCCCTTAGT CTCTCCAGAA 
GCCAAGGAGC TGGCTGAGGA GCGCATGCGA GCCAGGGAGA AGTCTGTGAA GAGCCAGGCG 
TTGAGAGACG CTATGGCCAA GCAGCTGAGT AAGATGAAGG AAATGGAGAT GGCGGCTGGG 
ACCTCCAGTC CCCCAGGAGG CACCTCCCAC AAAGCCTCCT CTGTTCCCTC CAAGGGCAAA 
GACCTCAGCC CCGAGTCCCC TAGACGCCCA GTTCTCAAAG GCCCCAGGGA GCCTGCCCTG 
AAGCACGAAG CCACTAGTGA GGAGGTCCTC TCCCCACCAT CGGACTCAGG GGGCCCAGAT 
GGTTCGGTCA CCTCATCCGA GGGCTCCAGT GGGAAGAGCA AGAAGAGATC GTCACTCTTC 
TCCCCCCGCA GAAACAAGAA AGAGAAGAAG TCCAAAGGTG AGGGCCGGCC CCCGGAGAAG 
CCCAGCCCAA GCCTTCTGGA GGAAGCTGCT GCCAAGCCCA AGTCCCTGTG GAAGTCGGTC 
TTTTCCGGGT ACAAGAAGGA CAAAAAGAAG AAGGGTGATG ACAAGTCCTG TTCCAGCACC 
CCTTCCAGCG GTGCCACTGT GGACTCTGGC AAGCACAAGA TGTCCCCTGT TGTGAGAGCA 
GAGCTGCACC TGCGGCGCCA GTTGAGCTTC TCCGAGGACT CAGACCTGTC CAGTGATGAC 
ATCCTTGAGA GGTCCTCCCA GAAGTCCAGG CGAGAGTCGG TTTATGTACC ACACGCCTTG 
GCATTCAAGA GATCATATCC ATCAAAGCCG AGAACCTACA CAGAGGAGGA ACTGAATGCC 
AAGCTGACCC GGCGTGTGCA GAAGGCAGCT CGTAGACAGG CCAAGCAAGA GGAGCTGAAG 
CGGCTGCACC GAGCACAGAT CATCCAGAGG CAGCTGGAGC AGGTGGAGGA GAAGCAGAGA 
CAGCTGGAGG AAAGAGGGGT GGCCGTGGAG AAGGCGCTCC GTGGTGAAGC AGACTATTGG 
GGAGAGTCTT ATTACAGTGA CCTCATCGAC TTGCATCTGG GTGGCATGGG CAAGAAGGAT 
GACCCCAAGC TGATGCAGGA GTGGTTCAAG TTGGTGCAAG AGAAGAATGC CATGGTGCGC 
TATGAGTCGG AGCTGATGAT TTTTGCCCGG GAGCTGGAAC TGGAGGACCG GCAGAGCCGA 
CTGCAGCAGG AGCTCCGGGA GCGGATGGCG GTGGAAGATC ACCTGAAGAC TGAGGAGGAG 
CTGTCTGAGG AGAAGAAGAT CTTGAACGAG ATGCTGGAGG TGGTGGAGCA GAGAGATGCC 
CTCGTGGCCC TGCTTGAGGA GCAGCGACTC CGGGAGAAGG AGGAGGACAA AGACCTGGAG 
GCTGTCATGC TGTCCAAGGG CTTCAGCCTG AACTGGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_022410881.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022266589.1 Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X12, mRNA.

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CloneID	OCe282677
Clone ID Related Accession (Same CDS sequence)	XM_022410881.1
Accession Version	XM_022410881.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6738bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	F-actin-methionine sulfoxide oxidase MICAL3 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGTTGGGA GCCAGAGGCT ACAGCAGATC 
GTAAATCCGG CCGACCCCTT GGAGATCCAG GCTGATGTGC ACTGGACACA TATCCGAGAG 
AGAGAGGAGG AGGAGCGAAT GGTCCCAACC TCTGAGTCCT CCACTTCTAG AGTGCCTGAC 
CTTCCCTCCA TACGCCGCGC AGCAGTGCAG GCCTGGCTGG AGACGGTCTC TGGAGTCCCA 
TTTGATGAGA ATGACCTGGA GGAAGACGTG GACTCGGAGC CAGCAAAGAT AGAAGGGGAG 
GCCACAGAGA ATGGGGACAC AGGGGACACT GGTGCTGAGC TGGACGATGA TCAGCGCTGG 
TCTGACGGGA TCCCATCGGA TGCCGAGACG GATCTCCGCG TGCAGCACCA GGAGGCGGTA 
GAGGCAGACC TGGAGCTGAG GGTCTCAGAG AATGAGGAGG AGCCACCTGA GGTCACACCA 
GGGCATCCGG AGAGAAGTCT CACCCGGGTC CCCAGCCCTG CCCTGTCCCC TGAGGAGCAA 
GCTGGTCTGC GGTCCCCAGG CCACAGCCCT AAGGCAGAGG GGGCCCAAAT CCCACTTGTC 
TCTGTTGTCA CCAAGGCAAA ATTGCCTGAA GAGAACCTTT TCCCCAAGCC TTTGCTCCCC 
AAAGTGAAGC CCAAGGTGGA AGTGCCCTGC AGTCAGAGAG CTGCATGCTC CCCCATCCGT 
TCTCAGCCAG TGGCTCTGCC AGAAGCCAGG ACACCTACCT CCCCAGTGAG CTCGCAGTGC 
CTGTCGCCTT TGGCCACCTC CACCCCCACC AGCACCCAGC TCCCCATTTG CTCCCAGCCT 
CAGCCTTCCT CTGAGACCAC GGCCCCATCC CCCACCAAGT CCCCCATACG GCTCCAGCCT 
GTTTCAGCCA AAACATCTAT CCCAGTGGTT CCCCTCCCCT TGAAGAACCA AGGGGACACC 
AAGGACAGGC TGGGCAGCCC TCTCGCTGTG GATGAGGCCC TGAAGCGGAA TGACCTGGTG 
GCAGAGTTCT GGATGAAGAG CGCCGAGATC CGCCGCAGCC TCGGGCTCAC ACCAGTGGAT 
CGGAGCAAGG TGTATGAGCC CAGCTTCCCT TCGCCTGCCT TTAAACCAGT GTCCCTAAAA 
TCCTATGCAG TGGAGAAGTC TCCCGAGGGG GAGGGACTCC AGCTTCTGAA GCCTCCACCT 
GTTCCTAAGA GACTAGGCCT GCCGAAGTCA GACGGTGACC AGCCCTTCCT GCCAACCCCC 
AAGTCCCCGT CGGACAGAGA GCTGAAAAGC TCCCAGGAGG AGAGGAGAGA TCTGTCGAGC 
AGCTCCGGGC TGGGCCTTCA CGGGAGCTCC TCCAACATGA AGACTTTGGG CAGCCAGAGC 
TTCAACACAT CAGACTCCAC CATGCTCACT CCCCCTTCAA GCCCACCGCC ACCACCACCC 
CAAGACGAGG AACCCGCAAC TCTTCGGAGG AAGCCATATC AGACTCATGA GCATAAGGAC 
GCTGAGCCCA AGGCCTCGGT GATTCCACCA CCACCACCTG CGACGTTCAT GCGGGCCCCC 
CGGGAGCCCA CCCAGCCCCT CCAAGAGGAG ATGCGGAAGT CGTTTGTGGA GAGCGTGGAT 
GAGATCCCAT TTGCCGATGA TGTGGAGGAC ACCTATGATG ACAAGACCGA GGACTCGAGT 
CTCCAGGAGA AGTTCTTCAC ACCCCCCTCC TACTGGCCCC GCACGGAGAG GCCCCTCCAC 
CCACCCCTAG CCAAGGAGAA TGGTAGGTTG CCTACTCTGG AGAGTGGGGT CCAGCCACAG 
AAGAGGGGCC TGCCCTTAGT CTCTCCAGAA GCCAAGGAGC TGGCTGAGGA GCGCATGCGA 
GCCAGGGAGA AGTCTGTGAA GAGCCAGGCG TTGAGAGACG CTATGGCCAA GCAGCTGAGT 
AAGATGAAGG AAATGGAGAT GGCGGCTGGG ACCTCCAGTC CCCCAGGAGG CACCTCCCAC 
AAAGCCTCCT CTGTTCCCTC CAAGGGCAAA GACCTCAGCC CCGAGTCCCC TAGACGCCCA 
GTTCTCAAAG GCCCCAGGGA GCCTGCCCTG AAGCACGAAG CCACTAGTGA GGAGGTCCTC 
TCCCCACCAT CGGACTCAGG GGGCCCAGAT GGTTCGGTCA CCTCATCCGA GGGCTCCAGT 
GGGAAGAGCA AGAAGAGATC GTCACTCTTC TCCCCCCGCA GAAACAAGAA AGAGAAGAAG 
TCCAAAGGTG AGGGCCGGCC CCCGGAGAAG CCCAGCCCAA GCCTTCTGGA GGAAGCTGCT 
GCCAAGCCCA AGTCCCTGTG GAAGTCGGTC TTTTCCGGGT ACAAGAAGGA CAAAAAGAAG 
AAGGGTGATG ACAAGTCCTG TTCCAGCACC CCTTCCAGCG GTGCCACTGT GGACTCTGGC 
AAGCACAAGA TGTCCCCTGT TGTGAGAGCA GAGCTGCACC TGCGGCGCCA GTTGAGCTTC 
TCCGAGGACT CAGACCTGTC CAGTGATGAC ATCCTTGAGA GGTCCTCCCA GAAGTCCAGG 
CGAGAGTCGG TTTATGTACC ACACGCCTTG GCATTCAAGA GATCATATCC ATCAAAGCCG 
AGAACCTACA CAGAGGAGGA ACTGAATGCC AAGCTGACCC GGCGTGTGCA GAAGGCAGCT 
CGTAGACAGG CCAAGCAAGA GGAGCTGAAG CGGCTGCACC GAGCACAGAT CATCCAGAGG 
CAGCTGGAGC AGGTGGAGGA GAAGCAGAGA CAGCTGGAGG AAAGAGGGGT GGCCGTGGAG 
AAGGCGCTCC GTGGTGAAGC AGACTATTGG GGAGAGTCTT ATTACAGTGA CCTCATCGAC 
TTGCATCTGG GTGTTGAGCC CAGTGGCGGG ACTCCACGGC GTAGACCTCT CTCCTTCTGC 
CCTTGCTGTG TCCAGGAAGG CATGGGCAAG AAGGATGACC CCAAGCTGAT GCAGGAGTGG 
TTCAAGTTGG TGCAAGAGAA GAATGCCATG GTGCGCTATG AGTCGGAGCT GATGATTTTT 
GCCCGGGAGC TGGAACTGGA GGACCGGCAG AGCCGACTGC AGCAGGAGCT CCGGGAGCGG 
ATGGCGGTGG AAGATCACCT GAAGACTGAG GAGGAGCTGT CTGAGGAGAA GAAGATCTTG 
AACGAGATGC TGGAGGTGGT GGAGCAGAGA GATGCCCTCG TGGCCCTGCT TGAGGAGCAG 
CGACTCCGGG AGAAGGAGGA GGACAAAGAC CTGGAGGCTG TCATGCTGTC CAAGGGCTTC 
AGCCTGAACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022266589.1
CDS	258..6995
Translation	MEDSKNETVNHAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDYRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKACTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFQGLVQPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELKEATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLNYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREQNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNININFLRPSQVRHLYNTGETRDIHLEMENLVNSRTTPKLTRNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGKEMASVGEPDKLSMVMYLTQFYEMFKDSLPSSDALDLNAEEKAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEEAPRGYRGGRPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSTGMRRQPTQERGVSQPSCCLPEQGHPAPTPQWKQQQEKERSRTCPKKVITLSPPLTPPPCRAPRSQQTYRDLDADSRGKQSPHHERPEPEPSRRFFVDQWELSLSLRSSNRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCRKRVYVMERLSAEGKFFHRSCFKCEYCATTLRLSAYAYDIEDGKFYCKPHYCYRLSGSAQRKRPAVAPLSGKEAREPLQDGPTVDTNGRASAVTSPAERTPGSSVNGVEEPSIAKRLRGTPERIELENYRLSVKQAEELEEVPEETQAEHNLSSVLDTGAEEDVASSSSESEMEEEEEEEPPLPTSDLGGVPWKEAVRIHALLKGKSEEELEASKSFGPGHEEDEEEEEYEEEEEEEEEEYEEEEEEEESSEVGSQRLQQIVNPADPLEIQADVHWTHIREREEEERMVPTSESSTSRVPDLPSIRRAAVQAWLETVSGVPFDENDLEEDVDSEPAKIEGEATENGDTGDTGAELDDDQRWSDGIPSDAETDLRVQHQEAVEADLELRVSENEEEPPEVTPGHPERSLTRVPSPALSPEEQAGLRSPGHSPKAEGAQIPLVSVVTKAKLPEENLFPKPLLPKVKPKVEVPCSQRAACSPIRSQPVALPEARTPTSPVSSQCLSPLATSTPTSTQLPICSQPQPSSETTAPSPTKSPIRLQPVSAKTSIPVVPLPLKNQGDTKDRLGSPLAVDEALKRNDLVAEFWMKSAEIRRSLGLTPVDRSKVYEPSFPSPAFKPVSLKSYAVEKSPEGEGLQLLKPPPVPKRLGLPKSDGDQPFLPTPKSPSDRELKSSQEERRDLSSSSGLGLHGSSSNMKTLGSQSFNTSDSTMLTPPSSPPPPPPQDEEPATLRRKPYQTHEHKDAEPKASVIPPPPPATFMRAPREPTQPLQEEMRKSFVESVDEIPFADDVEDTYDDKTEDSSLQEKFFTPPSYWPRTERPLHPPLAKENGRLPTLESGVQPQKRGLPLVSPEAKELAEERMRAREKSVKSQALRDAMAKQLSKMKEMEMAAGTSSPPGGTSHKASSVPSKGKDLSPESPRRPVLKGPREPALKHEATSEEVLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRNKKEKKSKGEGRPPEKPSPSLLEEAAAKPKSLWKSVFSGYKKDKKKKGDDKSCSSTPSSGATVDSGKHKMSPVVRAELHLRRQLSFSEDSDLSSDDILERSSQKSRRESVYVPHALAFKRSYPSKPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELSEEKKILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAVMLSKGFSLNWS

Target ORF information:

RefSeq Version	XM_022410881.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022410881.1

ORF Insert Sequence:

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGTTGGGA GCCAGAGGCT ACAGCAGATC 
GTAAATCCGG CCGACCCCTT GGAGATCCAG GCTGATGTGC ACTGGACACA TATCCGAGAG 
AGAGAGGAGG AGGAGCGAAT GGTCCCAACC TCTGAGTCCT CCACTTCTAG AGTGCCTGAC 
CTTCCCTCCA TACGCCGCGC AGCAGTGCAG GCCTGGCTGG AGACGGTCTC TGGAGTCCCA 
TTTGATGAGA ATGACCTGGA GGAAGACGTG GACTCGGAGC CAGCAAAGAT AGAAGGGGAG 
GCCACAGAGA ATGGGGACAC AGGGGACACT GGTGCTGAGC TGGACGATGA TCAGCGCTGG 
TCTGACGGGA TCCCATCGGA TGCCGAGACG GATCTCCGCG TGCAGCACCA GGAGGCGGTA 
GAGGCAGACC TGGAGCTGAG GGTCTCAGAG AATGAGGAGG AGCCACCTGA GGTCACACCA 
GGGCATCCGG AGAGAAGTCT CACCCGGGTC CCCAGCCCTG CCCTGTCCCC TGAGGAGCAA 
GCTGGTCTGC GGTCCCCAGG CCACAGCCCT AAGGCAGAGG GGGCCCAAAT CCCACTTGTC 
TCTGTTGTCA CCAAGGCAAA ATTGCCTGAA GAGAACCTTT TCCCCAAGCC TTTGCTCCCC 
AAAGTGAAGC CCAAGGTGGA AGTGCCCTGC AGTCAGAGAG CTGCATGCTC CCCCATCCGT 
TCTCAGCCAG TGGCTCTGCC AGAAGCCAGG ACACCTACCT CCCCAGTGAG CTCGCAGTGC 
CTGTCGCCTT TGGCCACCTC CACCCCCACC AGCACCCAGC TCCCCATTTG CTCCCAGCCT 
CAGCCTTCCT CTGAGACCAC GGCCCCATCC CCCACCAAGT CCCCCATACG GCTCCAGCCT 
GTTTCAGCCA AAACATCTAT CCCAGTGGTT CCCCTCCCCT TGAAGAACCA AGGGGACACC 
AAGGACAGGC TGGGCAGCCC TCTCGCTGTG GATGAGGCCC TGAAGCGGAA TGACCTGGTG 
GCAGAGTTCT GGATGAAGAG CGCCGAGATC CGCCGCAGCC TCGGGCTCAC ACCAGTGGAT 
CGGAGCAAGG TGTATGAGCC CAGCTTCCCT TCGCCTGCCT TTAAACCAGT GTCCCTAAAA 
TCCTATGCAG TGGAGAAGTC TCCCGAGGGG GAGGGACTCC AGCTTCTGAA GCCTCCACCT 
GTTCCTAAGA GACTAGGCCT GCCGAAGTCA GACGGTGACC AGCCCTTCCT GCCAACCCCC 
AAGTCCCCGT CGGACAGAGA GCTGAAAAGC TCCCAGGAGG AGAGGAGAGA TCTGTCGAGC 
AGCTCCGGGC TGGGCCTTCA CGGGAGCTCC TCCAACATGA AGACTTTGGG CAGCCAGAGC 
TTCAACACAT CAGACTCCAC CATGCTCACT CCCCCTTCAA GCCCACCGCC ACCACCACCC 
CAAGACGAGG AACCCGCAAC TCTTCGGAGG AAGCCATATC AGACTCATGA GCATAAGGAC 
GCTGAGCCCA AGGCCTCGGT GATTCCACCA CCACCACCTG CGACGTTCAT GCGGGCCCCC 
CGGGAGCCCA CCCAGCCCCT CCAAGAGGAG ATGCGGAAGT CGTTTGTGGA GAGCGTGGAT 
GAGATCCCAT TTGCCGATGA TGTGGAGGAC ACCTATGATG ACAAGACCGA GGACTCGAGT 
CTCCAGGAGA AGTTCTTCAC ACCCCCCTCC TACTGGCCCC GCACGGAGAG GCCCCTCCAC 
CCACCCCTAG CCAAGGAGAA TGGTAGGTTG CCTACTCTGG AGAGTGGGGT CCAGCCACAG 
AAGAGGGGCC TGCCCTTAGT CTCTCCAGAA GCCAAGGAGC TGGCTGAGGA GCGCATGCGA 
GCCAGGGAGA AGTCTGTGAA GAGCCAGGCG TTGAGAGACG CTATGGCCAA GCAGCTGAGT 
AAGATGAAGG AAATGGAGAT GGCGGCTGGG ACCTCCAGTC CCCCAGGAGG CACCTCCCAC 
AAAGCCTCCT CTGTTCCCTC CAAGGGCAAA GACCTCAGCC CCGAGTCCCC TAGACGCCCA 
GTTCTCAAAG GCCCCAGGGA GCCTGCCCTG AAGCACGAAG CCACTAGTGA GGAGGTCCTC 
TCCCCACCAT CGGACTCAGG GGGCCCAGAT GGTTCGGTCA CCTCATCCGA GGGCTCCAGT 
GGGAAGAGCA AGAAGAGATC GTCACTCTTC TCCCCCCGCA GAAACAAGAA AGAGAAGAAG 
TCCAAAGGTG AGGGCCGGCC CCCGGAGAAG CCCAGCCCAA GCCTTCTGGA GGAAGCTGCT 
GCCAAGCCCA AGTCCCTGTG GAAGTCGGTC TTTTCCGGGT ACAAGAAGGA CAAAAAGAAG 
AAGGGTGATG ACAAGTCCTG TTCCAGCACC CCTTCCAGCG GTGCCACTGT GGACTCTGGC 
AAGCACAAGA TGTCCCCTGT TGTGAGAGCA GAGCTGCACC TGCGGCGCCA GTTGAGCTTC 
TCCGAGGACT CAGACCTGTC CAGTGATGAC ATCCTTGAGA GGTCCTCCCA GAAGTCCAGG 
CGAGAGTCGG TTTATGTACC ACACGCCTTG GCATTCAAGA GATCATATCC ATCAAAGCCG 
AGAACCTACA CAGAGGAGGA ACTGAATGCC AAGCTGACCC GGCGTGTGCA GAAGGCAGCT 
CGTAGACAGG CCAAGCAAGA GGAGCTGAAG CGGCTGCACC GAGCACAGAT CATCCAGAGG 
CAGCTGGAGC AGGTGGAGGA GAAGCAGAGA CAGCTGGAGG AAAGAGGGGT GGCCGTGGAG 
AAGGCGCTCC GTGGTGAAGC AGACTATTGG GGAGAGTCTT ATTACAGTGA CCTCATCGAC 
TTGCATCTGG GTGTTGAGCC CAGTGGCGGG ACTCCACGGC GTAGACCTCT CTCCTTCTGC 
CCTTGCTGTG TCCAGGAAGG CATGGGCAAG AAGGATGACC CCAAGCTGAT GCAGGAGTGG 
TTCAAGTTGG TGCAAGAGAA GAATGCCATG GTGCGCTATG AGTCGGAGCT GATGATTTTT 
GCCCGGGAGC TGGAACTGGA GGACCGGCAG AGCCGACTGC AGCAGGAGCT CCGGGAGCGG 
ATGGCGGTGG AAGATCACCT GAAGACTGAG GAGGAGCTGT CTGAGGAGAA GAAGATCTTG 
AACGAGATGC TGGAGGTGGT GGAGCAGAGA GATGCCCTCG TGGCCCTGCT TGAGGAGCAG 
CGACTCCGGG AGAAGGAGGA GGACAAAGAC CTGGAGGCTG TCATGCTGTC CAAGGGCTTC 
AGCCTGAACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_022410882.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022266590.1 Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X13, mRNA.

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CloneID	OCe282678
Clone ID Related Accession (Same CDS sequence)	XM_022410882.1
Accession Version	XM_022410882.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	F-actin-methionine sulfoxide oxidase MICAL3 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GAAGTATATA AAGATGTACA CGGGCGGAGT GAGCTCATTG 
GCTGAGCAGA TAGCCAATCA GCTTCAAAGG AAAGAACAGC CCAAAACCCT TCTAGACAAG 
AAGGAACTGG GCTCGATAAA GAAGGAGTTC CCACAGAACT TAGGAGGCAG CGACACGTGC 
TATTTCTGCC GGAAGCGGGT CTACGTGATG GAAAGGCTGA GTGCTGAGGG CAAGTTCTTC 
CATCGGAGCT GCTTCAAGTG CGAGTACTGC GCCACCACCC TGCGCCTCTC GGCCTATGCC 
TATGACATTG AGGACGGTAA ATTCTACTGC AAACCACACT ACTGTTATCG ACTCTCTGGC 
TCTGCACAGA GGAAGAGACC AGCAGTGGCT CCTCTCTCTG GAAAGGAGGC CAGAGAACCC 
CTGCAGGATG GCCCCACTGT GGACACAAAT GGACGGGCCA GCGCTGTCAC CAGCCCTGCT 
GAGAGAACTC CAGGTTCAAG CGTGAATGGT GTGGAAGAGC CTAGCATTGC CAAGAGGCTC 
AGGGGCACTC CCGAGCGGAT TGAGCTGGAG AACTACCGCC TGTCCGTGAA GCAGGCCGAG 
GAGCTGGAGG AGGTGCCCGA GGAGACACAG GCCGAGCACA ACCTGAGCAG CGTGCTGGAC 
ACGGGGGCAG AGGAGGACGT GGCCAGCAGC AGTTCTGAGT CAGAGATGGA GGAAGAGGAG 
GAAGAAGAGC CTCCTCTGCC CACTTCGGAC CTGGGCGGTG TTCCTTGGAA GGAGGCCGTG 
AGGATCCATG CTCTTCTGAA AGGAAAGAGT GAAGAGGAGC TAGAGGCCTC AAAGAGCTTT 
GGGCCTGGGC ATGAAGAAGA CGAGGAGGAG GAGGAGTATG AAGAGGAGGA GGAGGAGGAG 
GAGGAAGAAT ATGAAGAGGA AGAGGAAGAG GAAGAGTCTA GTGAAGAAGG GGAGTATTGC 
CCCTGGGACA GAGAACTCCT ACAAGGCCAG TGGCTCCGGC AGCTGTCACA GGAAGAAACT 
ACGGGTACAC GTAAAGTTGG GAGCCAGAGG CTACAGCAGA TCGTAAATCC GGCCGACCCC 
TTGGAGATCC AGGCTGATGT GCACTGGACA CATATCCGAG AGAGAGAGGA GGAGGAGCGA 
ATGGTCCCAA CCTCTGAGTC CTCCACTTCT AGAGTGCCTG ACCTTCCCTC CATACGCCGC 
GCAGCAGTGC AGGCCTGGCT GGAGACGGTC TCTGGAGTCC CATTTGATGA GAATGACCTG 
GAGGAAGACG TGGACTCGGA GCCAGCAAAG ATAGAAGGGG AGGCCACAGA GAATGGGGAC 
ACAGGGGACA CTGGTGCTGA GCTGGACGAT GATCAGCGCT GGTCTGACGG GATCCCATCG 
GATGCCGAGA CGGATCTCCG CGTGCAGCAC CAGGAGGCGG TAGAGGCAGA CCTGGAGCTG 
AGGGTCTCAG AGAATGAGGA GGAGCCACCT GAGGTCACAC CAGGGCATCC GGAGAGAAGT 
CTCACCCGGG TCCCCAGCCC TGCCCTGTCC CCTGAGGAGC AAGCTGGTCT GCGGTCCCCA 
GGCCACAGCC CTAAGGCAGA GGGGGCCCAA ATCCCACTTG TCTCTGTTGT CACCAAGGCA 
AAATTGCCTG AAGAGAACCT TTTCCCCAAG CCTTTGCTCC CCAAAGTGAA GCCCAAGGTG 
GAAGTGCCCT GCAGTCAGAG AGCTGCATGC TCCCCCATCC GTTCTCAGCC AGTGGCTCTG 
CCAGAAGCCA GGACACCTAC CTCCCCAGTG AGCTCGCAGT GCCTGTCGCC TTTGGCCACC 
TCCACCCCCA CCAGCACCCA GCTCCCCATT TGCTCCCAGC CTCAGCCTTC CTCTGAGACC 
ACGGCCCCAT CCCCCACCAA GTCCCCCATA CGGCTCCAGC CTGTTTCAGC CAAAACATCT 
ATCCCAGTGG TTCCCCTCCC CTTGAAGAAC CAAGGGGACA CCAAGGACAG GCTGGGCAGC 
CCTCTCGCTG TGGATGAGGC CCTGAAGCGG AATGACCTGG TGGCAGAGTT CTGGATGAAG 
AGCGCCGAGA TCCGCCGCAG CCTCGGGCTC ACACCAGTGG ATCGGAGCAA GGTGTATGAG 
CCCAGCTTCC CTTCGCCTGC CTTTAAACCA GTGTCCCTAA AATCCTATGC AGTGGAGAAG 
TCTCCCGAGG GGGAGGGACT CCAGCTTCTG AAGCCTCCAC CTGTTCCTAA GAGACTAGGC 
CTGCCGAAGT CAGACGGTGA CCAGCCCTTC CTGCCAACCC CCAAGTCCCC GTCGGACAGA 
GAGCTGAAAA GCTCCCAGGA GGAGAGGAGA GATCTGTCGA GCAGCTCCGG GCTGGGCCTT 
CACGGGAGCT CCTCCAACAT GAAGACTTTG GGCAGCCAGA GCTTCAACAC ATCAGACTCC 
ACCATGCTCA CTCCCCCTTC AAGCCCACCG CCACCACCAC CCCAAGACGA GGAACCCGCA 
ACTCTTCGGA GGAAGCCATA TCAGACTCAT GAGCATAAGG ACGCTGAGCC CAAGGCCTCG 
GTGATTCCAC CACCACCACC TGCGACGTTC ATGCGGGCCC CCCGGGAGCC CACCCAGCCC 
CTCCAAGAGG AGATGCGGAA GTCGTTTGTG GAGAGCGTGG ATGAGATCCC ATTTGCCGAT 
GATGTGGAGG ACACCTATGA TGACAAGACC GAGGACTCGA GTCTCCAGGA GAAGTTCTTC 
ACACCCCCCT CCTACTGGCC CCGCACGGAG AGGCCCCTCC ACCCACCCCT AGCCAAGGAG 
AATGGTAGGT TGCCTACTCT GGAGAGTGGG GTCCAGCCAC AGAAGAGGGG CCTGCCCTTA 
GTCTCTCCAG AAGCCAAGGA GCTGGCTGAG GAGCGCATGC GAGCCAGGGA GAAGTCTGTG 
AAGAGCCAGG CGTTGAGAGA CGCTATGGCC AAGCAGCTGA GTAAGATGAA GGAAATGGAG 
ATGGCGGCTG GGACCTCCAG TCCCCCAGGA GGCACCTCCC ACAAAGCCTC CTCTGTTCCC 
TCCAAGGGCA AAGACCTCAG CCCCGAGTCC CCTAGACGCC CAGTTCTCAA AGGCCCCAGG 
GAGCCTGCCC TGAAGCACGA AGCCACTAGT GAGGAGGTCC TCTCCCCACC ATCGGACTCA 
GGGGGCCCAG ATGGTTCGGT CACCTCATCC GAGGGCTCCA GTGGGAAGAG CAAGAAGAGA 
TCGTCACTCT TCTCCCCCCG CAGAAACAAG AAAGAGAAGA AGTCCAAAGG TGAGGGCCGG 
CCCCCGGAGA AGCCCAGCCC AAGCCTTCTG GAGGAAGCTG CTGCCAAGCC CAAGTCCCTG 
TGGAAGTCGG TCTTTTCCGG GTACAAGAAG GACAAAAAGA AGAAGGGTGA TGACAAGTCC 
TGTTCCAGCA CCCCTTCCAG CGGTGCCACT GTGGACTCTG GCAAGCACAA GATGTCCCCT 
GTTGTGAGAG CAGAGCTGCA CCTGCGGCGC CAGTTGAGCT TCTCCGAGGA CTCAGACCTG 
TCCAGTGATG ACATCCTTGA GAGGTCCTCC CAGAAGTCCA GGCGAGAGTC GGTTTATGTA 
CCACACGCCT TGGCATTCAA GAGATCATAT CCATCAAAGC CGAGAACCTA CACAGAGGAG 
GAACTGAATG CCAAGCTGAC CCGGCGTGTG CAGAAGGCAG CTCGTAGACA GGCCAAGCAA 
GAGGAGCTGA AGCGGCTGCA CCGAGCACAG ATCATCCAGA GGCAGCTGGA GCAGGTGGAG 
GAGAAGCAGA GACAGCTGGA GGAAAGAGGG GTGGCCGTGG AGAAGGCGCT CCGTGGTGAA 
GCAGACTATT GGGGAGAGTC TTATTACAGT GACCTCATCG ACTTGCATCT GGGTGTTGAG 
CCCAGTGGCG GGACTCCACG GCGTAGACCT CTCTCCTTCT GCCCTTGCTG TGTCCAGGAA 
GGCATGGGCA AGAAGGATGA CCCCAAGCTG ATGCAGGAGT GGTTCAAGTT GGTGCAAGAG 
AAGAATGCCA TGGTGCGCTA TGAGTCGGAG CTGATGATTT TTGCCCGGGA GCTGGAACTG 
GAGGACCGGC AGAGCCGACT GCAGCAGGAG CTCCGGGAGC GGATGGCGGT GGAAGATCAC 
CTGAAGACTG AGGAGGAGCT GTCTGAGGAG AAGAAGATCT TGAACGAGAT GCTGGAGGTG 
GTGGAGCAGA GAGATGCCCT CGTGGCCCTG CTTGAGGAGC AGCGACTCCG GGAGAAGGAG 
GAGGACAAAG ACCTGGAGGC TGTCATGCTG TCCAAGGGCT TCAGCCTGAA CTGGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022266590.1
CDS	258..6977
Translation	MEDSKNETVNHAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDYRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKACTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFQGLVQPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELKEATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLNYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREQNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNININFLRPSQVRHLYNTGETRDIHLEMENLVNSRTTPKLTRNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGKEMASVGEPDKLSMVMYLTQFYEMFKDSLPSSDALDLNAEEKAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEEAPRGYRGGRPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSTGMRRQPTQERGVSQPSCCLPEQGHPAPTPQWKQQQEKERSRTCPKKVITLSPPLTPPPCRAPRSQQTYRDLDADSRGKQSPHHERKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCRKRVYVMERLSAEGKFFHRSCFKCEYCATTLRLSAYAYDIEDGKFYCKPHYCYRLSGSAQRKRPAVAPLSGKEAREPLQDGPTVDTNGRASAVTSPAERTPGSSVNGVEEPSIAKRLRGTPERIELENYRLSVKQAEELEEVPEETQAEHNLSSVLDTGAEEDVASSSSESEMEEEEEEEPPLPTSDLGGVPWKEAVRIHALLKGKSEEELEASKSFGPGHEEDEEEEEYEEEEEEEEEEYEEEEEEEESSEEGEYCPWDRELLQGQWLRQLSQEETTGTRKVGSQRLQQIVNPADPLEIQADVHWTHIREREEEERMVPTSESSTSRVPDLPSIRRAAVQAWLETVSGVPFDENDLEEDVDSEPAKIEGEATENGDTGDTGAELDDDQRWSDGIPSDAETDLRVQHQEAVEADLELRVSENEEEPPEVTPGHPERSLTRVPSPALSPEEQAGLRSPGHSPKAEGAQIPLVSVVTKAKLPEENLFPKPLLPKVKPKVEVPCSQRAACSPIRSQPVALPEARTPTSPVSSQCLSPLATSTPTSTQLPICSQPQPSSETTAPSPTKSPIRLQPVSAKTSIPVVPLPLKNQGDTKDRLGSPLAVDEALKRNDLVAEFWMKSAEIRRSLGLTPVDRSKVYEPSFPSPAFKPVSLKSYAVEKSPEGEGLQLLKPPPVPKRLGLPKSDGDQPFLPTPKSPSDRELKSSQEERRDLSSSSGLGLHGSSSNMKTLGSQSFNTSDSTMLTPPSSPPPPPPQDEEPATLRRKPYQTHEHKDAEPKASVIPPPPPATFMRAPREPTQPLQEEMRKSFVESVDEIPFADDVEDTYDDKTEDSSLQEKFFTPPSYWPRTERPLHPPLAKENGRLPTLESGVQPQKRGLPLVSPEAKELAEERMRAREKSVKSQALRDAMAKQLSKMKEMEMAAGTSSPPGGTSHKASSVPSKGKDLSPESPRRPVLKGPREPALKHEATSEEVLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRNKKEKKSKGEGRPPEKPSPSLLEEAAAKPKSLWKSVFSGYKKDKKKKGDDKSCSSTPSSGATVDSGKHKMSPVVRAELHLRRQLSFSEDSDLSSDDILERSSQKSRRESVYVPHALAFKRSYPSKPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELSEEKKILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAVMLSKGFSLNWS

Target ORF information:

RefSeq Version	XM_022410882.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022410882.1

ORF Insert Sequence:

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GAAGTATATA AAGATGTACA CGGGCGGAGT GAGCTCATTG 
GCTGAGCAGA TAGCCAATCA GCTTCAAAGG AAAGAACAGC CCAAAACCCT TCTAGACAAG 
AAGGAACTGG GCTCGATAAA GAAGGAGTTC CCACAGAACT TAGGAGGCAG CGACACGTGC 
TATTTCTGCC GGAAGCGGGT CTACGTGATG GAAAGGCTGA GTGCTGAGGG CAAGTTCTTC 
CATCGGAGCT GCTTCAAGTG CGAGTACTGC GCCACCACCC TGCGCCTCTC GGCCTATGCC 
TATGACATTG AGGACGGTAA ATTCTACTGC AAACCACACT ACTGTTATCG ACTCTCTGGC 
TCTGCACAGA GGAAGAGACC AGCAGTGGCT CCTCTCTCTG GAAAGGAGGC CAGAGAACCC 
CTGCAGGATG GCCCCACTGT GGACACAAAT GGACGGGCCA GCGCTGTCAC CAGCCCTGCT 
GAGAGAACTC CAGGTTCAAG CGTGAATGGT GTGGAAGAGC CTAGCATTGC CAAGAGGCTC 
AGGGGCACTC CCGAGCGGAT TGAGCTGGAG AACTACCGCC TGTCCGTGAA GCAGGCCGAG 
GAGCTGGAGG AGGTGCCCGA GGAGACACAG GCCGAGCACA ACCTGAGCAG CGTGCTGGAC 
ACGGGGGCAG AGGAGGACGT GGCCAGCAGC AGTTCTGAGT CAGAGATGGA GGAAGAGGAG 
GAAGAAGAGC CTCCTCTGCC CACTTCGGAC CTGGGCGGTG TTCCTTGGAA GGAGGCCGTG 
AGGATCCATG CTCTTCTGAA AGGAAAGAGT GAAGAGGAGC TAGAGGCCTC AAAGAGCTTT 
GGGCCTGGGC ATGAAGAAGA CGAGGAGGAG GAGGAGTATG AAGAGGAGGA GGAGGAGGAG 
GAGGAAGAAT ATGAAGAGGA AGAGGAAGAG GAAGAGTCTA GTGAAGAAGG GGAGTATTGC 
CCCTGGGACA GAGAACTCCT ACAAGGCCAG TGGCTCCGGC AGCTGTCACA GGAAGAAACT 
ACGGGTACAC GTAAAGTTGG GAGCCAGAGG CTACAGCAGA TCGTAAATCC GGCCGACCCC 
TTGGAGATCC AGGCTGATGT GCACTGGACA CATATCCGAG AGAGAGAGGA GGAGGAGCGA 
ATGGTCCCAA CCTCTGAGTC CTCCACTTCT AGAGTGCCTG ACCTTCCCTC CATACGCCGC 
GCAGCAGTGC AGGCCTGGCT GGAGACGGTC TCTGGAGTCC CATTTGATGA GAATGACCTG 
GAGGAAGACG TGGACTCGGA GCCAGCAAAG ATAGAAGGGG AGGCCACAGA GAATGGGGAC 
ACAGGGGACA CTGGTGCTGA GCTGGACGAT GATCAGCGCT GGTCTGACGG GATCCCATCG 
GATGCCGAGA CGGATCTCCG CGTGCAGCAC CAGGAGGCGG TAGAGGCAGA CCTGGAGCTG 
AGGGTCTCAG AGAATGAGGA GGAGCCACCT GAGGTCACAC CAGGGCATCC GGAGAGAAGT 
CTCACCCGGG TCCCCAGCCC TGCCCTGTCC CCTGAGGAGC AAGCTGGTCT GCGGTCCCCA 
GGCCACAGCC CTAAGGCAGA GGGGGCCCAA ATCCCACTTG TCTCTGTTGT CACCAAGGCA 
AAATTGCCTG AAGAGAACCT TTTCCCCAAG CCTTTGCTCC CCAAAGTGAA GCCCAAGGTG 
GAAGTGCCCT GCAGTCAGAG AGCTGCATGC TCCCCCATCC GTTCTCAGCC AGTGGCTCTG 
CCAGAAGCCA GGACACCTAC CTCCCCAGTG AGCTCGCAGT GCCTGTCGCC TTTGGCCACC 
TCCACCCCCA CCAGCACCCA GCTCCCCATT TGCTCCCAGC CTCAGCCTTC CTCTGAGACC 
ACGGCCCCAT CCCCCACCAA GTCCCCCATA CGGCTCCAGC CTGTTTCAGC CAAAACATCT 
ATCCCAGTGG TTCCCCTCCC CTTGAAGAAC CAAGGGGACA CCAAGGACAG GCTGGGCAGC 
CCTCTCGCTG TGGATGAGGC CCTGAAGCGG AATGACCTGG TGGCAGAGTT CTGGATGAAG 
AGCGCCGAGA TCCGCCGCAG CCTCGGGCTC ACACCAGTGG ATCGGAGCAA GGTGTATGAG 
CCCAGCTTCC CTTCGCCTGC CTTTAAACCA GTGTCCCTAA AATCCTATGC AGTGGAGAAG 
TCTCCCGAGG GGGAGGGACT CCAGCTTCTG AAGCCTCCAC CTGTTCCTAA GAGACTAGGC 
CTGCCGAAGT CAGACGGTGA CCAGCCCTTC CTGCCAACCC CCAAGTCCCC GTCGGACAGA 
GAGCTGAAAA GCTCCCAGGA GGAGAGGAGA GATCTGTCGA GCAGCTCCGG GCTGGGCCTT 
CACGGGAGCT CCTCCAACAT GAAGACTTTG GGCAGCCAGA GCTTCAACAC ATCAGACTCC 
ACCATGCTCA CTCCCCCTTC AAGCCCACCG CCACCACCAC CCCAAGACGA GGAACCCGCA 
ACTCTTCGGA GGAAGCCATA TCAGACTCAT GAGCATAAGG ACGCTGAGCC CAAGGCCTCG 
GTGATTCCAC CACCACCACC TGCGACGTTC ATGCGGGCCC CCCGGGAGCC CACCCAGCCC 
CTCCAAGAGG AGATGCGGAA GTCGTTTGTG GAGAGCGTGG ATGAGATCCC ATTTGCCGAT 
GATGTGGAGG ACACCTATGA TGACAAGACC GAGGACTCGA GTCTCCAGGA GAAGTTCTTC 
ACACCCCCCT CCTACTGGCC CCGCACGGAG AGGCCCCTCC ACCCACCCCT AGCCAAGGAG 
AATGGTAGGT TGCCTACTCT GGAGAGTGGG GTCCAGCCAC AGAAGAGGGG CCTGCCCTTA 
GTCTCTCCAG AAGCCAAGGA GCTGGCTGAG GAGCGCATGC GAGCCAGGGA GAAGTCTGTG 
AAGAGCCAGG CGTTGAGAGA CGCTATGGCC AAGCAGCTGA GTAAGATGAA GGAAATGGAG 
ATGGCGGCTG GGACCTCCAG TCCCCCAGGA GGCACCTCCC ACAAAGCCTC CTCTGTTCCC 
TCCAAGGGCA AAGACCTCAG CCCCGAGTCC CCTAGACGCC CAGTTCTCAA AGGCCCCAGG 
GAGCCTGCCC TGAAGCACGA AGCCACTAGT GAGGAGGTCC TCTCCCCACC ATCGGACTCA 
GGGGGCCCAG ATGGTTCGGT CACCTCATCC GAGGGCTCCA GTGGGAAGAG CAAGAAGAGA 
TCGTCACTCT TCTCCCCCCG CAGAAACAAG AAAGAGAAGA AGTCCAAAGG TGAGGGCCGG 
CCCCCGGAGA AGCCCAGCCC AAGCCTTCTG GAGGAAGCTG CTGCCAAGCC CAAGTCCCTG 
TGGAAGTCGG TCTTTTCCGG GTACAAGAAG GACAAAAAGA AGAAGGGTGA TGACAAGTCC 
TGTTCCAGCA CCCCTTCCAG CGGTGCCACT GTGGACTCTG GCAAGCACAA GATGTCCCCT 
GTTGTGAGAG CAGAGCTGCA CCTGCGGCGC CAGTTGAGCT TCTCCGAGGA CTCAGACCTG 
TCCAGTGATG ACATCCTTGA GAGGTCCTCC CAGAAGTCCA GGCGAGAGTC GGTTTATGTA 
CCACACGCCT TGGCATTCAA GAGATCATAT CCATCAAAGC CGAGAACCTA CACAGAGGAG 
GAACTGAATG CCAAGCTGAC CCGGCGTGTG CAGAAGGCAG CTCGTAGACA GGCCAAGCAA 
GAGGAGCTGA AGCGGCTGCA CCGAGCACAG ATCATCCAGA GGCAGCTGGA GCAGGTGGAG 
GAGAAGCAGA GACAGCTGGA GGAAAGAGGG GTGGCCGTGG AGAAGGCGCT CCGTGGTGAA 
GCAGACTATT GGGGAGAGTC TTATTACAGT GACCTCATCG ACTTGCATCT GGGTGTTGAG 
CCCAGTGGCG GGACTCCACG GCGTAGACCT CTCTCCTTCT GCCCTTGCTG TGTCCAGGAA 
GGCATGGGCA AGAAGGATGA CCCCAAGCTG ATGCAGGAGT GGTTCAAGTT GGTGCAAGAG 
AAGAATGCCA TGGTGCGCTA TGAGTCGGAG CTGATGATTT TTGCCCGGGA GCTGGAACTG 
GAGGACCGGC AGAGCCGACT GCAGCAGGAG CTCCGGGAGC GGATGGCGGT GGAAGATCAC 
CTGAAGACTG AGGAGGAGCT GTCTGAGGAG AAGAAGATCT TGAACGAGAT GCTGGAGGTG 
GTGGAGCAGA GAGATGCCCT CGTGGCCCTG CTTGAGGAGC AGCGACTCCG GGAGAAGGAG 
GAGGACAAAG ACCTGGAGGC TGTCATGCTG TCCAAGGGCT TCAGCCTGAA CTGGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_022410883.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022266591.1 Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X14, mRNA.

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CloneID	OCe282679
Clone ID Related Accession (Same CDS sequence)	XM_022410883.1
Accession Version	XM_022410883.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	F-actin-methionine sulfoxide oxidase MICAL3 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTGCCTGAC CTTCCCTCCA TACGCCGCGC AGCAGTGCAG 
GCCTGGCTGG AGACGGTCTC TGGAGTCCCA TTTGATGAGA ATGACCTGGA GGAAGACGTG 
GACTCGGAGC CAGCAAAGAT AGAAGGGGAG GCCACAGAGA ATGGGGACAC AGGGGACACT 
GGTGCTGAGC TGGACGATGA TCAGCGCTGG TCTGACGGGA TCCCATCGGA TGCCGAGACG 
GATCTCCGCG TGCAGCACCA GGAGGCGGTA GAGGCAGACC TGGAGCTGAG GGTCTCAGAG 
AATGAGGAGG AGCCACCTGA GGTCACACCA GGGCATCCGG AGAGAAGTCT CACCCGGGTC 
CCCAGCCCTG CCCTGTCCCC TGAGGAGCAA GCTGGTCTGC GGTCCCCAGG CCACAGCCCT 
AAGGCAGAGG GGGCCCAAAT CCCACTTGTC TCTGTTGTCA CCAAGGCAAA ATTGCCTGAA 
GAGAACCTTT TCCCCAAGCC TTTGCTCCCC AAAGTGAAGC CCAAGGTGGA AGTGCCCTGC 
AGTCAGAGAG CTGCATGCTC CCCCATCCGT TCTCAGCCAG TGGCTCTGCC AGAAGCCAGG 
ACACCTACCT CCCCAGTGAG CTCGCAGTGC CTGTCGCCTT TGGCCACCTC CACCCCCACC 
AGCACCCAGC TCCCCATTTG CTCCCAGCCT CAGCCTTCCT CTGAGACCAC GGCCCCATCC 
CCCACCAAGT CCCCCATACG GCTCCAGCCT GTTTCAGCCA AAACATCTAT CCCAGTGGTT 
CCCCTCCCCT TGAAGAACCA AGGGGACACC AAGGACAGGC TGGGCAGCCC TCTCGCTGTG 
GATGAGGCCC TGAAGCGGAA TGACCTGGTG GCAGAGTTCT GGATGAAGAG CGCCGAGATC 
CGCCGCAGCC TCGGGCTCAC ACCAGTGGAT CGGAGCAAGG TGTATGAGCC CAGCTTCCCT 
TCGCCTGCCT TTAAACCAGT GTCCCTAAAA TCCTATGCAG TGGAGAAGTC TCCCGAGGGG 
GAGGGACTCC AGCTTCTGAA GCCTCCACCT GTTCCTAAGA GACTAGGCCT GCCGAAGTCA 
GACGGTGACC AGCCCTTCCT GCCAACCCCC AAGTCCCCGT CGGACAGAGA GCTGAAAAGC 
TCCCAGGAGG AGAGGAGAGA TCTGTCGAGC AGCTCCGGGC TGGGCCTTCA CGGGAGCTCC 
TCCAACATGA AGACTTTGGG CAGCCAGAGC TTCAACACAT CAGACTCCAC CATGCTCACT 
CCCCCTTCAA GCCCACCGCC ACCACCACCC CAAGACGAGG AACCCGCAAC TCTTCGGAGG 
AAGCCATATC AGACTCATGA GCATAAGGAC GCTGAGCCCA AGGCCTCGGT GATTCCACCA 
CCACCACCTG CGACGTTCAT GCGGGCCCCC CGGGAGCCCA CCCAGCCCCT CCAAGAGGAG 
ATGCGGAAGT CGTTTGTGGA GAGCGTGGAT GAGATCCCAT TTGCCGATGA TGTGGAGGAC 
ACCTATGATG ACAAGACCGA GGACTCGAGT CTCCAGGAGA AGTTCTTCAC ACCCCCCTCC 
TACTGGCCCC GCACGGAGAG GCCCCTCCAC CCACCCCTAG CCAAGGAGAA TGGTAGGTTG 
CCTACTCTGG AGAGTGGGGT CCAGCCACAG AAGAGGGGCC TGCCCTTAGT CTCTCCAGAA 
GCCAAGGAGC TGGCTGAGGA GCGCATGCGA GCCAGGGAGA AGTCTGTGAA GAGCCAGGCG 
TTGAGAGACG CTATGGCCAA GCAGCTGAGT AAGATGAAGG AAATGGAGAT GGCGGCTGGG 
ACCTCCAGTC CCCCAGGAGG CACCTCCCAC AAAGCCTCCT CTGTTCCCTC CAAGGGCAAA 
GACCTCAGCC CCGAGTCCCC TAGACGCCCA GTTCTCAAAG GCCCCAGGGA GCCTGCCCTG 
AAGCACGAAG CCACTAGTGA GGAGGTCCTC TCCCCACCAT CGGACTCAGG GGGCCCAGAT 
GGTTCGGTCA CCTCATCCGA GGGCTCCAGT GGGAAGAGCA AGAAGAGATC GTCACTCTTC 
TCCCCCCGCA GAAACAAGAA AGAGAAGAAG TCCAAAGGTG AGGGCCGGCC CCCGGAGAAG 
CCCAGCCCAA GCCTTCTGGA GGAAGCTGCT GCCAAGCCCA AGTCCCTGTG GAAGTCGGTC 
TTTTCCGGGT ACAAGAAGGA CAAAAAGAAG AAGGGTGATG ACAAGTCCTG TTCCAGCACC 
CCTTCCAGCG GTGCCACTGT GGACTCTGGC AAGCACAAGA TGTCCCCTGT TGTGAGAGCA 
GAGCTGCACC TGCGGCGCCA GTTGAGCTTC TCCGAGGACT CAGACCTGTC CAGTGATGAC 
ATCCTTGAGA GGTCCTCCCA GAAGTCCAGG CGAGAGTCGG TTTATGTACC ACACGCCTTG 
GCATTCAAGA GATCATATCC ATCAAAGCCG AGAACCTACA CAGAGGAGGA ACTGAATGCC 
AAGCTGACCC GGCGTGTGCA GAAGGCAGCT CGTAGACAGG CCAAGCAAGA GGAGCTGAAG 
CGGCTGCACC GAGCACAGAT CATCCAGAGG CAGCTGGAGC AGGTGGAGGA GAAGCAGAGA 
CAGCTGGAGG AAAGAGGGGT GGCCGTGGAG AAGGCGCTCC GTGGTGAAGC AGGCATGGGC 
AAGAAGGATG ACCCCAAGCT GATGCAGGAG TGGTTCAAGT TGGTGCAAGA GAAGAATGCC 
ATGGTGCGCT ATGAGTCGGA GCTGATGATT TTTGCCCGGG AGCTGGAACT GGAGGACCGG 
CAGAGCCGAC TGCAGCAGGA GCTCCGGGAG CGGATGGCGG TGGAAGATCA CCTGAAGACT 
GAGGAGGAGC TGTCTGAGGA GAAGAAGATC TTGAACGAGA TGCTGGAGGT GGTGGAGCAG 
AGAGATGCCC TCGTGGCCCT GCTTGAGGAG CAGCGACTCC GGGAGAAGGA GGAGGACAAA 
GACCTGGAGG CTGTCATGCT GTCCAAGGGC TTCAGCCTGA ACTGGTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022266591.1
CDS	258..6968
Translation	MEDSKNETVNHAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDYRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKACTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFQGLVQPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELKEATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLNYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREQNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNININFLRPSQVRHLYNTGETRDIHLEMENLVNSRTTPKLTRNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGKEMASVGEPDKLSMVMYLTQFYEMFKDSLPSSDALDLNAEEKAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEEAPRGYRGGRPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSTGMRRQPTQERGVSQPSCCLPEQGHPAPTPQWKQQQEKERSRTCPKKVITLSPPLTPPPCRAPRSQQTYRDLDADSRGKQSPHHERPEPEPSRRFFVDQWELSLSLRSSNRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCRKRVYVMERLSAEGKFFHRSCFKCEYCATTLRLSAYAYDIEDGKFYCKPHYCYRLSGSAQRKRPAVAPLSGKEAREPLQDGPTVDTNGRASAVTSPAERTPGSSVNGVEEPSIAKRLRGTPERIELENYRLSVKQAEELEEVPEETQAEHNLSSVLDTGAEEDVASSSSESEMEEEEEEEPPLPTSDLGGVPWKEAVRIHALLKGKSEEELEASKSFGPGHEEDEEEEEYEEEEEEEEEEYEEEEEEEESSEEGEYCPWDRELLQGQWLRQLSQEETTGTRKVGSQRLQQIVNPADPLEIQADVHWTHIREREEEERMVPTSESSTSRVPDLPSIRRAAVQAWLETVSGVPFDENDLEEDVDSEPAKIEGEATENGDTGDTGAELDDDQRWSDGIPSDAETDLRVQHQEAVEADLELRVSENEEEPPEVTPGHPERSLTRVPSPALSPEEQAGLRSPGHSPKAEGAQIPLVSVVTKAKLPEENLFPKPLLPKVKPKVEVPCSQRAACSPIRSQPVALPEARTPTSPVSSQCLSPLATSTPTSTQLPICSQPQPSSETTAPSPTKSPIRLQPVSAKTSIPVVPLPLKNQGDTKDRLGSPLAVDEALKRNDLVAEFWMKSAEIRRSLGLTPVDRSKVYEPSFPSPAFKPVSLKSYAVEKSPEGEGLQLLKPPPVPKRLGLPKSDGDQPFLPTPKSPSDRELKSSQEERRDLSSSSGLGLHGSSSNMKTLGSQSFNTSDSTMLTPPSSPPPPPPQDEEPATLRRKPYQTHEHKDAEPKASVIPPPPPATFMRAPREPTQPLQEEMRKSFVESVDEIPFADDVEDTYDDKTEDSSLQEKFFTPPSYWPRTERPLHPPLAKENGRLPTLESGVQPQKRGLPLVSPEAKELAEERMRAREKSVKSQALRDAMAKQLSKMKEMEMAAGTSSPPGGTSHKASSVPSKGKDLSPESPRRPVLKGPREPALKHEATSEEVLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRNKKEKKSKGEGRPPEKPSPSLLEEAAAKPKSLWKSVFSGYKKDKKKKGDDKSCSSTPSSGATVDSGKHKMSPVVRAELHLRRQLSFSEDSDLSSDDILERSSQKSRRESVYVPHALAFKRSYPSKPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELSEEKKILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAVMLSKGFSLNWS

Target ORF information:

RefSeq Version	XM_022410883.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022410883.1

ORF Insert Sequence:

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTGCCTGAC CTTCCCTCCA TACGCCGCGC AGCAGTGCAG 
GCCTGGCTGG AGACGGTCTC TGGAGTCCCA TTTGATGAGA ATGACCTGGA GGAAGACGTG 
GACTCGGAGC CAGCAAAGAT AGAAGGGGAG GCCACAGAGA ATGGGGACAC AGGGGACACT 
GGTGCTGAGC TGGACGATGA TCAGCGCTGG TCTGACGGGA TCCCATCGGA TGCCGAGACG 
GATCTCCGCG TGCAGCACCA GGAGGCGGTA GAGGCAGACC TGGAGCTGAG GGTCTCAGAG 
AATGAGGAGG AGCCACCTGA GGTCACACCA GGGCATCCGG AGAGAAGTCT CACCCGGGTC 
CCCAGCCCTG CCCTGTCCCC TGAGGAGCAA GCTGGTCTGC GGTCCCCAGG CCACAGCCCT 
AAGGCAGAGG GGGCCCAAAT CCCACTTGTC TCTGTTGTCA CCAAGGCAAA ATTGCCTGAA 
GAGAACCTTT TCCCCAAGCC TTTGCTCCCC AAAGTGAAGC CCAAGGTGGA AGTGCCCTGC 
AGTCAGAGAG CTGCATGCTC CCCCATCCGT TCTCAGCCAG TGGCTCTGCC AGAAGCCAGG 
ACACCTACCT CCCCAGTGAG CTCGCAGTGC CTGTCGCCTT TGGCCACCTC CACCCCCACC 
AGCACCCAGC TCCCCATTTG CTCCCAGCCT CAGCCTTCCT CTGAGACCAC GGCCCCATCC 
CCCACCAAGT CCCCCATACG GCTCCAGCCT GTTTCAGCCA AAACATCTAT CCCAGTGGTT 
CCCCTCCCCT TGAAGAACCA AGGGGACACC AAGGACAGGC TGGGCAGCCC TCTCGCTGTG 
GATGAGGCCC TGAAGCGGAA TGACCTGGTG GCAGAGTTCT GGATGAAGAG CGCCGAGATC 
CGCCGCAGCC TCGGGCTCAC ACCAGTGGAT CGGAGCAAGG TGTATGAGCC CAGCTTCCCT 
TCGCCTGCCT TTAAACCAGT GTCCCTAAAA TCCTATGCAG TGGAGAAGTC TCCCGAGGGG 
GAGGGACTCC AGCTTCTGAA GCCTCCACCT GTTCCTAAGA GACTAGGCCT GCCGAAGTCA 
GACGGTGACC AGCCCTTCCT GCCAACCCCC AAGTCCCCGT CGGACAGAGA GCTGAAAAGC 
TCCCAGGAGG AGAGGAGAGA TCTGTCGAGC AGCTCCGGGC TGGGCCTTCA CGGGAGCTCC 
TCCAACATGA AGACTTTGGG CAGCCAGAGC TTCAACACAT CAGACTCCAC CATGCTCACT 
CCCCCTTCAA GCCCACCGCC ACCACCACCC CAAGACGAGG AACCCGCAAC TCTTCGGAGG 
AAGCCATATC AGACTCATGA GCATAAGGAC GCTGAGCCCA AGGCCTCGGT GATTCCACCA 
CCACCACCTG CGACGTTCAT GCGGGCCCCC CGGGAGCCCA CCCAGCCCCT CCAAGAGGAG 
ATGCGGAAGT CGTTTGTGGA GAGCGTGGAT GAGATCCCAT TTGCCGATGA TGTGGAGGAC 
ACCTATGATG ACAAGACCGA GGACTCGAGT CTCCAGGAGA AGTTCTTCAC ACCCCCCTCC 
TACTGGCCCC GCACGGAGAG GCCCCTCCAC CCACCCCTAG CCAAGGAGAA TGGTAGGTTG 
CCTACTCTGG AGAGTGGGGT CCAGCCACAG AAGAGGGGCC TGCCCTTAGT CTCTCCAGAA 
GCCAAGGAGC TGGCTGAGGA GCGCATGCGA GCCAGGGAGA AGTCTGTGAA GAGCCAGGCG 
TTGAGAGACG CTATGGCCAA GCAGCTGAGT AAGATGAAGG AAATGGAGAT GGCGGCTGGG 
ACCTCCAGTC CCCCAGGAGG CACCTCCCAC AAAGCCTCCT CTGTTCCCTC CAAGGGCAAA 
GACCTCAGCC CCGAGTCCCC TAGACGCCCA GTTCTCAAAG GCCCCAGGGA GCCTGCCCTG 
AAGCACGAAG CCACTAGTGA GGAGGTCCTC TCCCCACCAT CGGACTCAGG GGGCCCAGAT 
GGTTCGGTCA CCTCATCCGA GGGCTCCAGT GGGAAGAGCA AGAAGAGATC GTCACTCTTC 
TCCCCCCGCA GAAACAAGAA AGAGAAGAAG TCCAAAGGTG AGGGCCGGCC CCCGGAGAAG 
CCCAGCCCAA GCCTTCTGGA GGAAGCTGCT GCCAAGCCCA AGTCCCTGTG GAAGTCGGTC 
TTTTCCGGGT ACAAGAAGGA CAAAAAGAAG AAGGGTGATG ACAAGTCCTG TTCCAGCACC 
CCTTCCAGCG GTGCCACTGT GGACTCTGGC AAGCACAAGA TGTCCCCTGT TGTGAGAGCA 
GAGCTGCACC TGCGGCGCCA GTTGAGCTTC TCCGAGGACT CAGACCTGTC CAGTGATGAC 
ATCCTTGAGA GGTCCTCCCA GAAGTCCAGG CGAGAGTCGG TTTATGTACC ACACGCCTTG 
GCATTCAAGA GATCATATCC ATCAAAGCCG AGAACCTACA CAGAGGAGGA ACTGAATGCC 
AAGCTGACCC GGCGTGTGCA GAAGGCAGCT CGTAGACAGG CCAAGCAAGA GGAGCTGAAG 
CGGCTGCACC GAGCACAGAT CATCCAGAGG CAGCTGGAGC AGGTGGAGGA GAAGCAGAGA 
CAGCTGGAGG AAAGAGGGGT GGCCGTGGAG AAGGCGCTCC GTGGTGAAGC AGGCATGGGC 
AAGAAGGATG ACCCCAAGCT GATGCAGGAG TGGTTCAAGT TGGTGCAAGA GAAGAATGCC 
ATGGTGCGCT ATGAGTCGGA GCTGATGATT TTTGCCCGGG AGCTGGAACT GGAGGACCGG 
CAGAGCCGAC TGCAGCAGGA GCTCCGGGAG CGGATGGCGG TGGAAGATCA CCTGAAGACT 
GAGGAGGAGC TGTCTGAGGA GAAGAAGATC TTGAACGAGA TGCTGGAGGT GGTGGAGCAG 
AGAGATGCCC TCGTGGCCCT GCTTGAGGAG CAGCGACTCC GGGAGAAGGA GGAGGACAAA 
GACCTGGAGG CTGTCATGCT GTCCAAGGGC TTCAGCCTGA ACTGGTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_022410884.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022266592.1 Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X15, mRNA.

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CloneID	OCe282680
Clone ID Related Accession (Same CDS sequence)	XM_022410884.1
Accession Version	XM_022410884.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	F-actin-methionine sulfoxide oxidase MICAL3 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTGCCTGAC CTTCCCTCCA TACGCCGCGC AGCAGTGCAG 
GCCTGGCTGG AGACGGTCTC TGGAGTCCCA TTTGATGAGA ATGACCTGGA GGAAGACGTG 
GACTCGGAGC CAGCAAAGAT AGAAGGGGAG GCCACAGAGA ATGGGGACAC AGGGGACACT 
GGTGCTGAGC TGGACGATGA TCAGCGCTGG TCTGACGGGA TCCCATCGGA TGCCGAGACG 
GATCTCCGCG TGCAGCACCA GGAGGCGGTA GAGGCAGACC TGGAGCTGAG GGTCTCAGAG 
AATGAGGAGG AGCCACCTGA GGTCACACCA GGGCATCCGG AGAGAAGTCT CACCCGGGTC 
CCCAGCCCTG CCCTGTCCCC TGAGGAGCAA GCTGGTCTGC GGTCCCCAGG CCACAGCCCT 
AAGGCAGAGG GGGCCCAAAT CCCACTTGTC TCTGTTGTCA CCAAGGCAAA ATTGCCTGAA 
GAGAACCTTT TCCCCAAGCC TTTGCTCCCC AAAGTGAAGC CCAAGGTGGA AGTGCCCTGC 
AGTCAGAGAG CTGCATGCTC CCCCATCCGT TCTCAGCCAG TGGCTCTGCC AGAAGCCAGG 
ACACCTACCT CCCCAGTGAG CTCGCAGTGC CTGTCGCCTT TGGCCACCTC CACCCCCACC 
AGCACCCAGC TCCCCATTTG CTCCCAGCCT CAGCCTTCCT CTGAGACCAC GGCCCCATCC 
CCCACCAAGT CCCCCATACG GCTCCAGCCT GTTTCAGCCA AAACATCTAT CCCAGTGGTT 
CCCCTCCCCT TGAAGAACCA AGGGGACACC AAGGACAGGC TGGGCAGCCC TCTCGCTGTG 
GATGAGGCCC TGAAGCGGAA TGACCTGGTG GCAGAGTTCT GGATGAAGAG CGCCGAGATC 
CGCCGCAGCC TCGGGCTCAC ACCAGTGGAT CGGAGCAAGG TGTATGAGCC CAGCTTCCCT 
TCGCCTGCCT TTAAACCAGT GTCCCTAAAA TCCTATGCAG TGGAGAAGTC TCCCGAGGGG 
GAGGGACTCC AGCTTCTGAA GCCTCCACCT GTTCCTAAGA GACTAGGCCT GCCGAAGTCA 
GACGGTGACC AGCCCTTCCT GCCAACCCCC AAGTCCCCGT CGGACAGAGA GCTGAAAAGC 
TCCCAGGAGG AGAGGAGAGA TCTGTCGAGC AGCTCCGGGC TGGGCCTTCA CGGGAGCTCC 
TCCAACATGA AGACTTTGGG CAGCCAGAGC TTCAACACAT CAGACTCCAC CATGCTCACT 
CCCCCTTCAA GCCCACCGCC ACCACCACCC CAAGACGAGG AACCCGCAAC TCTTCGGAGG 
AAGCCATATC AGACTCATGA GCATAAGGAC GCTGAGCCCA AGGCCTCGGT GATTCCACCA 
CCACCACCTG CGACGTTCAT GCGGGCCCCC CGGGAGCCCA CCCAGCCCCT CCAAGAGGAG 
ATGCGGAAGT CGTTTGTGGA GAGCGTGGAT GAGATCCCAT TTGCCGATGA TGTGGAGGAC 
ACCTATGATG ACAAGACCGA GGACTCGAGT CTCCAGGAGA AGTTCTTCAC ACCCCCCTCC 
TACTGGCCCC GCACGGAGAG GCCCCTCCAC CCACCCCTAG CCAAGGAGAA TGGTAGGTTG 
CCTACTCTGG AGAGTGGGGT CCAGCCACAG AAGAGGGGCC TGCCCTTAGT CTCTCCAGAA 
GCCAAGGAGC TGGCTGAGGA GCGCATGCGA GCCAGGGAGA AGTCTGTGAA GAGCCAGGCG 
TTGAGAGACG CTATGGCCAA GCAGCTGAGT AAGATGAAGG AAATGGAGAT GGCGGCTGGG 
ACCTCCAGTC CCCCAGGAGG CACCTCCCAC AAAGCCTCCT CTGTTCCCTC CAAGGGCAAA 
GACCTCAGCC CCGAGTCCCC TAGACGCCCA GTTCTCAAAG GCCCCAGGGA GCCTGCCCTG 
AAGCACGAAG CCACTAGTGA GGAGGTCCTC TCCCCACCAT CGGACTCAGG GGGCCCAGAT 
GGTTCGGTCA CCTCATCCGA GGGCTCCAGT GGGAAGAGCA AGAAGAGATC GTCACTCTTC 
TCCCCCCGCA GAAACAAGAA AGAGAAGAAG TCCAAAGGTG AGGGCCGGCC CCCGGAGAAG 
CCCAGCCCAA GCCTTCTGGA GGAAGCTGCT GCCAAGCCCA AGTCCCTGTG GAAGTCGGTC 
TTTTCCGGGT ACAAGAAGGA CAAAAAGAAG AAGGGTGATG ACAAGTCCTG TTCCAGCACC 
CCTTCCAGCG GTGCCACTGT GGACTCTGGC AAGCACAAGA TGTCCCCTGT TGTGAGAGCA 
GAGCTGCACC TGCGGCGCCA GTTGAGCTTC TCCGAGGACT CAGACCTGTC CAGTGATGAC 
ATCCTTGAGA GGTCCTCCCA GAAGTCCAGG CGAGAGCCGA GAACCTACAC AGAGGAGGAA 
CTGAATGCCA AGCTGACCCG GCGTGTGCAG AAGGCAGCTC GTAGACAGGC CAAGCAAGAG 
GAGCTGAAGC GGCTGCACCG AGCACAGATC ATCCAGAGGC AGCTGGAGCA GGTGGAGGAG 
AAGCAGAGAC AGCTGGAGGA AAGAGGGGTG GCCGTGGAGA AGGCGCTCCG TGGTGAAGCA 
GGCATGGGCA AGAAGGATGA CCCCAAGCTG ATGCAGGAGT GGTTCAAGTT GGTGCAAGAG 
AAGAATGCCA TGGTGCGCTA TGAGTCGGAG CTGATGATTT TTGCCCGGGA GCTGGAACTG 
GAGGACCGGC AGAGCCGACT GCAGCAGGAG CTCCGGGAGC GGATGGCGGT GGAAGATCAC 
CTGAAGACTG AGGAGGAGCT GTCTGAGGAG AAGAAGATCT TGAACGAGAT GCTGGAGGTG 
GTGGAGCAGA GAGATGCCCT CGTGGCCCTG CTTGAGGAGC AGCGACTCCG GGAGAAGGAG 
GAGGACAAAG ACCTGGAGGC TGTCATGCTG TCCAAGGGCT TCAGCCTGAA CTGGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022266592.1
CDS	258..6917
Translation	MEDSKNETVNHAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDYRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKACTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFQGLVQPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELKEATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLNYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREQNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNININFLRPSQVRHLYNTGETRDIHLEMENLVNSRTTPKLTRNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGKEMASVGEPDKLSMVMYLTQFYEMFKDSLPSSDALDLNAEEKAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEEAPRGYRGGRPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSTGMRRQPTQERGVSQPSCCLPEQGHPAPTPQWKQQQEKERSRTCPKKVITLSPPLTPPPCRAPRSQQTYRDLDADSRGKQSPHHERPEPEPSRRFFVDQWELSLSLRSSNRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCRKRVYVMERLSAEGKFFHRSCFKCEYCATTLRLSAYAYDIEDGKFYCKPHYCYRLSGSAQRKRPAVAPLSGKEAREPLQDGPTVDTNGRASAVTSPAERTPGSSVNGVEEPSIAKRLRGTPERIELENYRLSVKQAEELEEVPEETQAEHNLSSVLDTGAEEDVASSSSESEMEEEEEEEPPLPTSDLGGVPWKEAVRIHALLKGKSEEELEASKSFGPGHEEDEEEEEYEEEEEEEEEEYEEEEEEEESSEEGEYCPWDRELLQGQWLRQLSQEETTGTRKVGSQRLQQIVNPADPLEIQADVHWTHIREREEEERMVPTSESSTSRVPDLPSIRRAAVQAWLETVSGVPFDENDLEEDVDSEPAKIEGEATENGDTGDTGAELDDDQRWSDGIPSDAETDLRVQHQEAVEADLELRVSENEEEPPEVTPGHPERSLTRVPSPALSPEEQAGLRSPGHSPKAEGAQIPLVSVVTKAKLPEENLFPKPLLPKVKPKVEVPCSQRAACSPIRSQPVALPEARTPTSPVSSQCLSPLATSTPTSTQLPICSQPQPSSETTAPSPTKSPIRLQPVSAKTSIPVVPLPLKNQGDTKDRLGSPLAVDEALKRNDLVAEFWMKSAEIRRSLGLTPVDRSKVYEPSFPSPAFKPVSLKSYAVEKSPEGEGLQLLKPPPVPKRLGLPKSDGDQPFLPTPKSPSDRELKSSQEERRDLSSSSGLGLHGSSSNMKTLGSQSFNTSDSTMLTPPSSPPPPPPQDEEPATLRRKPYQTHEHKDAEPKASVIPPPPPATFMRAPREPTQPLQEEMRKSFVESVDEIPFADDVEDTYDDKTEDSSLQEKFFTPPSYWPRTERPLHPPLAKENGRLPTLESGVQPQKRGLPLVSPEAKELAEERMRAREKSVKSQALRDAMAKQLSKMKEMEMAAGTSSPPGGTSHKASSVPSKGKDLSPESPRRPVLKGPREPALKHEATSEEVLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRNKKEKKSKGEGRPPEKPSPSLLEEAAAKPKSLWKSVFSGYKKDKKKKGDDKSCSSTPSSGATVDSGKHKMSPVVRAELHLRRQLSFSEDSDLSSDDILERSSQKSRREPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELSEEKKILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAVMLSKGFSLNWS

Target ORF information:

RefSeq Version	XM_022410884.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022410884.1

ORF Insert Sequence:

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTGCCTGAC CTTCCCTCCA TACGCCGCGC AGCAGTGCAG 
GCCTGGCTGG AGACGGTCTC TGGAGTCCCA TTTGATGAGA ATGACCTGGA GGAAGACGTG 
GACTCGGAGC CAGCAAAGAT AGAAGGGGAG GCCACAGAGA ATGGGGACAC AGGGGACACT 
GGTGCTGAGC TGGACGATGA TCAGCGCTGG TCTGACGGGA TCCCATCGGA TGCCGAGACG 
GATCTCCGCG TGCAGCACCA GGAGGCGGTA GAGGCAGACC TGGAGCTGAG GGTCTCAGAG 
AATGAGGAGG AGCCACCTGA GGTCACACCA GGGCATCCGG AGAGAAGTCT CACCCGGGTC 
CCCAGCCCTG CCCTGTCCCC TGAGGAGCAA GCTGGTCTGC GGTCCCCAGG CCACAGCCCT 
AAGGCAGAGG GGGCCCAAAT CCCACTTGTC TCTGTTGTCA CCAAGGCAAA ATTGCCTGAA 
GAGAACCTTT TCCCCAAGCC TTTGCTCCCC AAAGTGAAGC CCAAGGTGGA AGTGCCCTGC 
AGTCAGAGAG CTGCATGCTC CCCCATCCGT TCTCAGCCAG TGGCTCTGCC AGAAGCCAGG 
ACACCTACCT CCCCAGTGAG CTCGCAGTGC CTGTCGCCTT TGGCCACCTC CACCCCCACC 
AGCACCCAGC TCCCCATTTG CTCCCAGCCT CAGCCTTCCT CTGAGACCAC GGCCCCATCC 
CCCACCAAGT CCCCCATACG GCTCCAGCCT GTTTCAGCCA AAACATCTAT CCCAGTGGTT 
CCCCTCCCCT TGAAGAACCA AGGGGACACC AAGGACAGGC TGGGCAGCCC TCTCGCTGTG 
GATGAGGCCC TGAAGCGGAA TGACCTGGTG GCAGAGTTCT GGATGAAGAG CGCCGAGATC 
CGCCGCAGCC TCGGGCTCAC ACCAGTGGAT CGGAGCAAGG TGTATGAGCC CAGCTTCCCT 
TCGCCTGCCT TTAAACCAGT GTCCCTAAAA TCCTATGCAG TGGAGAAGTC TCCCGAGGGG 
GAGGGACTCC AGCTTCTGAA GCCTCCACCT GTTCCTAAGA GACTAGGCCT GCCGAAGTCA 
GACGGTGACC AGCCCTTCCT GCCAACCCCC AAGTCCCCGT CGGACAGAGA GCTGAAAAGC 
TCCCAGGAGG AGAGGAGAGA TCTGTCGAGC AGCTCCGGGC TGGGCCTTCA CGGGAGCTCC 
TCCAACATGA AGACTTTGGG CAGCCAGAGC TTCAACACAT CAGACTCCAC CATGCTCACT 
CCCCCTTCAA GCCCACCGCC ACCACCACCC CAAGACGAGG AACCCGCAAC TCTTCGGAGG 
AAGCCATATC AGACTCATGA GCATAAGGAC GCTGAGCCCA AGGCCTCGGT GATTCCACCA 
CCACCACCTG CGACGTTCAT GCGGGCCCCC CGGGAGCCCA CCCAGCCCCT CCAAGAGGAG 
ATGCGGAAGT CGTTTGTGGA GAGCGTGGAT GAGATCCCAT TTGCCGATGA TGTGGAGGAC 
ACCTATGATG ACAAGACCGA GGACTCGAGT CTCCAGGAGA AGTTCTTCAC ACCCCCCTCC 
TACTGGCCCC GCACGGAGAG GCCCCTCCAC CCACCCCTAG CCAAGGAGAA TGGTAGGTTG 
CCTACTCTGG AGAGTGGGGT CCAGCCACAG AAGAGGGGCC TGCCCTTAGT CTCTCCAGAA 
GCCAAGGAGC TGGCTGAGGA GCGCATGCGA GCCAGGGAGA AGTCTGTGAA GAGCCAGGCG 
TTGAGAGACG CTATGGCCAA GCAGCTGAGT AAGATGAAGG AAATGGAGAT GGCGGCTGGG 
ACCTCCAGTC CCCCAGGAGG CACCTCCCAC AAAGCCTCCT CTGTTCCCTC CAAGGGCAAA 
GACCTCAGCC CCGAGTCCCC TAGACGCCCA GTTCTCAAAG GCCCCAGGGA GCCTGCCCTG 
AAGCACGAAG CCACTAGTGA GGAGGTCCTC TCCCCACCAT CGGACTCAGG GGGCCCAGAT 
GGTTCGGTCA CCTCATCCGA GGGCTCCAGT GGGAAGAGCA AGAAGAGATC GTCACTCTTC 
TCCCCCCGCA GAAACAAGAA AGAGAAGAAG TCCAAAGGTG AGGGCCGGCC CCCGGAGAAG 
CCCAGCCCAA GCCTTCTGGA GGAAGCTGCT GCCAAGCCCA AGTCCCTGTG GAAGTCGGTC 
TTTTCCGGGT ACAAGAAGGA CAAAAAGAAG AAGGGTGATG ACAAGTCCTG TTCCAGCACC 
CCTTCCAGCG GTGCCACTGT GGACTCTGGC AAGCACAAGA TGTCCCCTGT TGTGAGAGCA 
GAGCTGCACC TGCGGCGCCA GTTGAGCTTC TCCGAGGACT CAGACCTGTC CAGTGATGAC 
ATCCTTGAGA GGTCCTCCCA GAAGTCCAGG CGAGAGCCGA GAACCTACAC AGAGGAGGAA 
CTGAATGCCA AGCTGACCCG GCGTGTGCAG AAGGCAGCTC GTAGACAGGC CAAGCAAGAG 
GAGCTGAAGC GGCTGCACCG AGCACAGATC ATCCAGAGGC AGCTGGAGCA GGTGGAGGAG 
AAGCAGAGAC AGCTGGAGGA AAGAGGGGTG GCCGTGGAGA AGGCGCTCCG TGGTGAAGCA 
GGCATGGGCA AGAAGGATGA CCCCAAGCTG ATGCAGGAGT GGTTCAAGTT GGTGCAAGAG 
AAGAATGCCA TGGTGCGCTA TGAGTCGGAG CTGATGATTT TTGCCCGGGA GCTGGAACTG 
GAGGACCGGC AGAGCCGACT GCAGCAGGAG CTCCGGGAGC GGATGGCGGT GGAAGATCAC 
CTGAAGACTG AGGAGGAGCT GTCTGAGGAG AAGAAGATCT TGAACGAGAT GCTGGAGGTG 
GTGGAGCAGA GAGATGCCCT CGTGGCCCTG CTTGAGGAGC AGCGACTCCG GGAGAAGGAG 
GAGGACAAAG ACCTGGAGGC TGTCATGCTG TCCAAGGGCT TCAGCCTGAA CTGGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_022410885.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022266593.1 Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X16, mRNA.

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CloneID	OCe282681
Clone ID Related Accession (Same CDS sequence)	XM_022410885.1
Accession Version	XM_022410885.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	F-actin-methionine sulfoxide oxidase MICAL3 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGACGTA CAGGGATCTT 
GATGCTGACA GCCGTGGCAA GCAGAGTCCC CACCATGAGA GGAAGTATAT AAAGATGTAC 
ACGGGCGGAG TGAGCTCATT GGCTGAGCAG ATAGCCAATC AGCTTCAAAG GAAAGAACAG 
CCCAAAACCC TTCTAGACAA GAAGGAACTG GGCTCGATAA AGAAGGAGTT CCCACAGAAC 
TTAGGAGGCA GCGACACGTG CTATTTCTGC CGGAAGCGGG TCTACGTGAT GGAAAGGCTG 
AGTGCTGAGG GCAAGTTCTT CCATCGGAGC TGCTTCAAGT GCGAGTACTG CGCCACCACC 
CTGCGCCTCT CGGCCTATGC CTATGACATT GAGGACGGTA AATTCTACTG CAAACCACAC 
TACTGTTATC GACTCTCTGG CTCTGCACAG AGGAAGAGAC CAGCAGTGGC TCCTCTCTCT 
GGAAAGGAGG CCAGAGAACC CCTGCAGGAT GGCCCCACTG TGGACACAAA TGGACGGGCC 
AGCGCTGTCA CCAGCCCTGC TGAGAGAACT CCAGGTTCAA GCGTGAATGG TGTGGAAGAG 
CCTAGCATTG CCAAGAGGCT CAGGGGCACT CCCGAGCGGA TTGAGCTGGA GAACTACCGC 
CTGTCCGTGA AGCAGGCCGA GGAGCTGGAG GAGGTGCCCG AGGAGACACA GGCCGAGCAC 
AACCTGAGCA GCGTGCTGGA CACGGGGGCA GAGGAGGACG TGGCCAGCAG CAGTTCTGAG 
TCAGAGATGG AGGAAGAGGA GGAAGAAGAG CCTCCTCTGC CCACTTCGGA CCTGGGCGGT 
GTTCCTTGGA AGGAGGCCGT GAGGATCCAT GCTCTTCTGA AAGGAAAGAG TGAAGAGGAG 
CTAGAGGCCT CAAAGAGCTT TGGGCCTGGG CATGAAGAAG ACGAGGAGGA GGAGGAGTAT 
GAAGAGGAGG AGGAGGAGGA GGAGGAAGAA TATGAAGAGG AAGAGGAAGA GGAAGAGTCT 
AGTGAAGAAG GGGAGTATTG CCCCTGGGAC AGAGAACTCC TACAAGGCCA GTGGCTCCGG 
CAGCTGTCAC AGGAAGAAAC TACGGGTACA CGTAAAGTTG GGAGCCAGAG GCTACAGCAG 
ATCGTAAATC CGGCCGACCC CTTGGAGATC CAGGCTGATG TGCACTGGAC ACATATCCGA 
GAGAGAGAGG AGGAGGAGCG AATGGTCCCA ACCTCTGAGT CCTCCACTTC TAGAGTGCCT 
GACCTTCCCT CCATACGCCG CGCAGCAGTG CAGGCCTGGC TGGAGACGGT CTCTGGAGTC 
CCATTTGATG AGAATGACCT GGAGGAAGAC GTGGACTCGG AGCCAGCAAA GATAGAAGGG 
GAGGCCACAG AGAATGGGGA CACAGGGGAC ACTGGTGCTG AGCTGGACGA TGATCAGCGC 
TGGTCTGACG GGATCCCATC GGATGCCGAG ACGGATCTCC GCGTGCAGCA CCAGGAGGCG 
GTAGAGGCAG ACCTGGAGCT GAGGGTCTCA GAGAATGAGG AGGAGCCACC TGAGGTCACA 
CCAGGGCATC CGGAGAGAAG TCTCACCCGG GTCCCCAGCC CTGCCCTGTC CCCTGAGGAG 
CAAGCTGGTC TGCGGTCCCC AGGCCACAGC CCTAAGGCAG AGGGGGCCCA AATCCCACTT 
GTCTCTGTTG TCACCAAGGC AAAATTGCCT GAAGAGAACC TTTTCCCCAA GCCTTTGCTC 
CCCAAAGTGA AGCCCAAGGT GGAAGTGCCC TGCAGTCAGA GAGCTGCATG CTCCCCCATC 
CGTTCTCAGC CAGTGGCTCT GCCAGAAGCC AGGACACCTA CCTCCCCAGT GAGCTCGCAG 
TGCCTGTCGC CTTTGGCCAC CTCCACCCCC ACCAGCACCC AGCTCCCCAT TTGCTCCCAG 
CCTCAGCCTT CCTCTGAGAC CACGGCCCCA TCCCCCACCA AGTCCCCCAT ACGGCTCCAG 
CCTGTTTCAG CCAAAACATC TATCCCAGTG GTTCCCCTCC CCTTGAAGAA CCAAGGGGAC 
ACCAAGGACA GGCTGGGCAG CCCTCTCGCT GTGGATGAGG CCCTGAAGCG GAATGACCTG 
GTGGCAGAGT TCTGGATGAA GAGCGCCGAG ATCCGCCGCA GCCTCGGGCT CACACCAGTG 
GATCGGAGCA AGGTGTATGA GCCCAGCTTC CCTTCGCCTG CCTTTAAACC AGTGTCCCTA 
AAATCCTATG CAGTGGAGAA GTCTCCCGAG GGGGAGGGAC TCCAGCTTCT GAAGCCTCCA 
CCTGTTCCTA AGAGACTAGG CCTGCCGAAG TCAGACGGTG ACCAGCCCTT CCTGCCAACC 
CCCAAGTCCC CGTCGGACAG AGAGCTGAAA AGCTCCCAGG AGGAGAGGAG AGATCTGTCG 
AGCAGCTCCG GGCTGGGCCT TCACGGGAGC TCCTCCAACA TGAAGACTTT GGGCAGCCAG 
AGCTTCAACA CATCAGACTC CACCATGCTC ACTCCCCCTT CAAGCCCACC GCCACCACCA 
CCCCAAGACG AGGAACCCGC AACTCTTCGG AGGAAGCCAT ATCAGACTCA TGAGCATAAG 
GACGCTGAGC CCAAGGCCTC GGTGATTCCA CCACCACCAC CTGCGACGTT CATGCGGGCC 
CCCCGGGAGC CCACCCAGCC CCTCCAAGAG GAGATGCGGA AGTCGTTTGT GGAGAGCGTG 
GATGAGATCC CATTTGCCGA TGATGTGGAG GACACCTATG ATGACAAGAC CGAGGACTCG 
AGTCTCCAGG AGAAGTTCTT CACACCCCCC TCCTACTGGC CCCGCACGGA GAGGCCCCTC 
CACCCACCCC TAGCCAAGGA GAATGGTAGG TTGCCTACTC TGGAGAGTGG GGTCCAGCCA 
CAGAAGAGGG GCCTGCCCTT AGTCTCTCCA GAAGCCAAGG AGCTGGCTGA GGAGCGCATG 
CGAGCCAGGG AGAAGTCTGT GAAGAGCCAG GCGTTGAGAG ACGCTATGGC CAAGCAGCTG 
AGTAAGATGA AGGAAATGGA GATGGCGGCT GGGACCTCCA GTCCCCCAGG AGGCACCTCC 
CACAAAGCCT CCTCTGTTCC CTCCAAGGGC AAAGACCTCA GCCCCGAGTC CCCTAGACGC 
CCAGTTCTCA AAGGCCCCAG GGAGCCTGCC CTGAAGCACG AAGCCACTAG TGAGGAGGTC 
CTCTCCCCAC CATCGGACTC AGGGGGCCCA GATGGTTCGG TCACCTCATC CGAGGGCTCC 
AGTGGGAAGA GCAAGAAGAG ATCGTCACTC TTCTCCCCCC GCAGAAACAA GAAAGAGAAG 
AAGTCCAAAG GTGAGGGCCG GCCCCCGGAG AAGCCCAGCC CAAGCCTTCT GGAGGAAGCT 
GCTGCCAAGC CCAAGTCCCT GTGGAAGTCG GTCTTTTCCG GGTACAAGAA GGACAAAAAG 
AAGAAGGGTG ATGACAAGTC CTGTTCCAGC ACCCCTTCCA GCGGTGCCAC TGTGGACTCT 
GGCAAGCACA AGATGTCCCC TGTTGTGAGA GCAGAGCTGC ACCTGCGGCG CCAGTTGAGC 
TTCTCCGAGG ACTCAGACCT GTCCAGTGAT GACATCCTTG AGAGGTCCTC CCAGAAGTCC 
AGGCGAGAGT CGGTTTATGT ACCACACGCC TTGGCATTCA AGAGATCATA TCCATCAAAG 
CCGAGAACCT ACACAGAGGA GGAACTGAAT GCCAAGCTGA CCCGGCGTGT GCAGAAGGCA 
GCTCGTAGAC AGGCCAAGCA AGAGGAGCTG AAGCGGCTGC ACCGAGCACA GATCATCCAG 
AGGCAGCTGG AGCAGGTGGA GGAGAAGCAG AGACAGCTGG AGGAAAGAGG GGTGGCCGTG 
GAGAAGGCGC TCCGTGGTGA AGCAGACTAT TGGGGAGAGT CTTATTACAG TGACCTCATC 
GACTTGCATC TGGGTGTTGA GCCCAGTGGC GGGACTCCAC GGCGTAGACC TCTCTCCTTC 
TGCCCTTGCT GTGTCCAGGA AGGCATGGGC AAGAAGGATG ACCCCAAGCT GATGCAGGAG 
TGGTTCAAGT TGGTGCAAGA GAAGAATGCC ATGGTGCGCT ATGAGTCGGA GCTGATGATT 
TTTGCCCGGG AGCTGGAACT GGAGGACCGG CAGAGCCGAC TGCAGCAGGA GCTCCGGGAG 
CGGATGGCGG TGGAAGATCA CCTGAAGACT GAGGAGGAGC TGTCTGAGGA GAAGAAGATC 
TTGAACGAGA TGCTGGAGGT GGTGGAGCAG AGAGATGCCC TCGTGGCCCT GCTTGAGGAG 
CAGCGACTCC GGGAGAAGGA GGAGGACAAA GACCTGGAGG CTGTCATGCT GTCCAAGGGC 
TTCAGCCTGA ACTGGTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022266593.1
CDS	258..6878
Translation	MEDSKNETVNHAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDYRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKACTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFQGLVQPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELKEATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLNYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREQNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNININFLRPSQVRHLYNTGETRDIHLEMENLVNSRTTPKLTRNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGKEMASVGEPDKLSMVMYLTQFYEMFKDSLPSSDALDLNAEEKAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEEAPRGYRGGRPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSTGMRRQPTQERGVSQPSCCLPEQGHPAPTPQWKQTYRDLDADSRGKQSPHHERKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCRKRVYVMERLSAEGKFFHRSCFKCEYCATTLRLSAYAYDIEDGKFYCKPHYCYRLSGSAQRKRPAVAPLSGKEAREPLQDGPTVDTNGRASAVTSPAERTPGSSVNGVEEPSIAKRLRGTPERIELENYRLSVKQAEELEEVPEETQAEHNLSSVLDTGAEEDVASSSSESEMEEEEEEEPPLPTSDLGGVPWKEAVRIHALLKGKSEEELEASKSFGPGHEEDEEEEEYEEEEEEEEEEYEEEEEEEESSEEGEYCPWDRELLQGQWLRQLSQEETTGTRKVGSQRLQQIVNPADPLEIQADVHWTHIREREEEERMVPTSESSTSRVPDLPSIRRAAVQAWLETVSGVPFDENDLEEDVDSEPAKIEGEATENGDTGDTGAELDDDQRWSDGIPSDAETDLRVQHQEAVEADLELRVSENEEEPPEVTPGHPERSLTRVPSPALSPEEQAGLRSPGHSPKAEGAQIPLVSVVTKAKLPEENLFPKPLLPKVKPKVEVPCSQRAACSPIRSQPVALPEARTPTSPVSSQCLSPLATSTPTSTQLPICSQPQPSSETTAPSPTKSPIRLQPVSAKTSIPVVPLPLKNQGDTKDRLGSPLAVDEALKRNDLVAEFWMKSAEIRRSLGLTPVDRSKVYEPSFPSPAFKPVSLKSYAVEKSPEGEGLQLLKPPPVPKRLGLPKSDGDQPFLPTPKSPSDRELKSSQEERRDLSSSSGLGLHGSSSNMKTLGSQSFNTSDSTMLTPPSSPPPPPPQDEEPATLRRKPYQTHEHKDAEPKASVIPPPPPATFMRAPREPTQPLQEEMRKSFVESVDEIPFADDVEDTYDDKTEDSSLQEKFFTPPSYWPRTERPLHPPLAKENGRLPTLESGVQPQKRGLPLVSPEAKELAEERMRAREKSVKSQALRDAMAKQLSKMKEMEMAAGTSSPPGGTSHKASSVPSKGKDLSPESPRRPVLKGPREPALKHEATSEEVLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRNKKEKKSKGEGRPPEKPSPSLLEEAAAKPKSLWKSVFSGYKKDKKKKGDDKSCSSTPSSGATVDSGKHKMSPVVRAELHLRRQLSFSEDSDLSSDDILERSSQKSRRESVYVPHALAFKRSYPSKPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELSEEKKILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAVMLSKGFSLNWS

Target ORF information:

RefSeq Version	XM_022410885.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022410885.1

ORF Insert Sequence:

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGACGTA CAGGGATCTT 
GATGCTGACA GCCGTGGCAA GCAGAGTCCC CACCATGAGA GGAAGTATAT AAAGATGTAC 
ACGGGCGGAG TGAGCTCATT GGCTGAGCAG ATAGCCAATC AGCTTCAAAG GAAAGAACAG 
CCCAAAACCC TTCTAGACAA GAAGGAACTG GGCTCGATAA AGAAGGAGTT CCCACAGAAC 
TTAGGAGGCA GCGACACGTG CTATTTCTGC CGGAAGCGGG TCTACGTGAT GGAAAGGCTG 
AGTGCTGAGG GCAAGTTCTT CCATCGGAGC TGCTTCAAGT GCGAGTACTG CGCCACCACC 
CTGCGCCTCT CGGCCTATGC CTATGACATT GAGGACGGTA AATTCTACTG CAAACCACAC 
TACTGTTATC GACTCTCTGG CTCTGCACAG AGGAAGAGAC CAGCAGTGGC TCCTCTCTCT 
GGAAAGGAGG CCAGAGAACC CCTGCAGGAT GGCCCCACTG TGGACACAAA TGGACGGGCC 
AGCGCTGTCA CCAGCCCTGC TGAGAGAACT CCAGGTTCAA GCGTGAATGG TGTGGAAGAG 
CCTAGCATTG CCAAGAGGCT CAGGGGCACT CCCGAGCGGA TTGAGCTGGA GAACTACCGC 
CTGTCCGTGA AGCAGGCCGA GGAGCTGGAG GAGGTGCCCG AGGAGACACA GGCCGAGCAC 
AACCTGAGCA GCGTGCTGGA CACGGGGGCA GAGGAGGACG TGGCCAGCAG CAGTTCTGAG 
TCAGAGATGG AGGAAGAGGA GGAAGAAGAG CCTCCTCTGC CCACTTCGGA CCTGGGCGGT 
GTTCCTTGGA AGGAGGCCGT GAGGATCCAT GCTCTTCTGA AAGGAAAGAG TGAAGAGGAG 
CTAGAGGCCT CAAAGAGCTT TGGGCCTGGG CATGAAGAAG ACGAGGAGGA GGAGGAGTAT 
GAAGAGGAGG AGGAGGAGGA GGAGGAAGAA TATGAAGAGG AAGAGGAAGA GGAAGAGTCT 
AGTGAAGAAG GGGAGTATTG CCCCTGGGAC AGAGAACTCC TACAAGGCCA GTGGCTCCGG 
CAGCTGTCAC AGGAAGAAAC TACGGGTACA CGTAAAGTTG GGAGCCAGAG GCTACAGCAG 
ATCGTAAATC CGGCCGACCC CTTGGAGATC CAGGCTGATG TGCACTGGAC ACATATCCGA 
GAGAGAGAGG AGGAGGAGCG AATGGTCCCA ACCTCTGAGT CCTCCACTTC TAGAGTGCCT 
GACCTTCCCT CCATACGCCG CGCAGCAGTG CAGGCCTGGC TGGAGACGGT CTCTGGAGTC 
CCATTTGATG AGAATGACCT GGAGGAAGAC GTGGACTCGG AGCCAGCAAA GATAGAAGGG 
GAGGCCACAG AGAATGGGGA CACAGGGGAC ACTGGTGCTG AGCTGGACGA TGATCAGCGC 
TGGTCTGACG GGATCCCATC GGATGCCGAG ACGGATCTCC GCGTGCAGCA CCAGGAGGCG 
GTAGAGGCAG ACCTGGAGCT GAGGGTCTCA GAGAATGAGG AGGAGCCACC TGAGGTCACA 
CCAGGGCATC CGGAGAGAAG TCTCACCCGG GTCCCCAGCC CTGCCCTGTC CCCTGAGGAG 
CAAGCTGGTC TGCGGTCCCC AGGCCACAGC CCTAAGGCAG AGGGGGCCCA AATCCCACTT 
GTCTCTGTTG TCACCAAGGC AAAATTGCCT GAAGAGAACC TTTTCCCCAA GCCTTTGCTC 
CCCAAAGTGA AGCCCAAGGT GGAAGTGCCC TGCAGTCAGA GAGCTGCATG CTCCCCCATC 
CGTTCTCAGC CAGTGGCTCT GCCAGAAGCC AGGACACCTA CCTCCCCAGT GAGCTCGCAG 
TGCCTGTCGC CTTTGGCCAC CTCCACCCCC ACCAGCACCC AGCTCCCCAT TTGCTCCCAG 
CCTCAGCCTT CCTCTGAGAC CACGGCCCCA TCCCCCACCA AGTCCCCCAT ACGGCTCCAG 
CCTGTTTCAG CCAAAACATC TATCCCAGTG GTTCCCCTCC CCTTGAAGAA CCAAGGGGAC 
ACCAAGGACA GGCTGGGCAG CCCTCTCGCT GTGGATGAGG CCCTGAAGCG GAATGACCTG 
GTGGCAGAGT TCTGGATGAA GAGCGCCGAG ATCCGCCGCA GCCTCGGGCT CACACCAGTG 
GATCGGAGCA AGGTGTATGA GCCCAGCTTC CCTTCGCCTG CCTTTAAACC AGTGTCCCTA 
AAATCCTATG CAGTGGAGAA GTCTCCCGAG GGGGAGGGAC TCCAGCTTCT GAAGCCTCCA 
CCTGTTCCTA AGAGACTAGG CCTGCCGAAG TCAGACGGTG ACCAGCCCTT CCTGCCAACC 
CCCAAGTCCC CGTCGGACAG AGAGCTGAAA AGCTCCCAGG AGGAGAGGAG AGATCTGTCG 
AGCAGCTCCG GGCTGGGCCT TCACGGGAGC TCCTCCAACA TGAAGACTTT GGGCAGCCAG 
AGCTTCAACA CATCAGACTC CACCATGCTC ACTCCCCCTT CAAGCCCACC GCCACCACCA 
CCCCAAGACG AGGAACCCGC AACTCTTCGG AGGAAGCCAT ATCAGACTCA TGAGCATAAG 
GACGCTGAGC CCAAGGCCTC GGTGATTCCA CCACCACCAC CTGCGACGTT CATGCGGGCC 
CCCCGGGAGC CCACCCAGCC CCTCCAAGAG GAGATGCGGA AGTCGTTTGT GGAGAGCGTG 
GATGAGATCC CATTTGCCGA TGATGTGGAG GACACCTATG ATGACAAGAC CGAGGACTCG 
AGTCTCCAGG AGAAGTTCTT CACACCCCCC TCCTACTGGC CCCGCACGGA GAGGCCCCTC 
CACCCACCCC TAGCCAAGGA GAATGGTAGG TTGCCTACTC TGGAGAGTGG GGTCCAGCCA 
CAGAAGAGGG GCCTGCCCTT AGTCTCTCCA GAAGCCAAGG AGCTGGCTGA GGAGCGCATG 
CGAGCCAGGG AGAAGTCTGT GAAGAGCCAG GCGTTGAGAG ACGCTATGGC CAAGCAGCTG 
AGTAAGATGA AGGAAATGGA GATGGCGGCT GGGACCTCCA GTCCCCCAGG AGGCACCTCC 
CACAAAGCCT CCTCTGTTCC CTCCAAGGGC AAAGACCTCA GCCCCGAGTC CCCTAGACGC 
CCAGTTCTCA AAGGCCCCAG GGAGCCTGCC CTGAAGCACG AAGCCACTAG TGAGGAGGTC 
CTCTCCCCAC CATCGGACTC AGGGGGCCCA GATGGTTCGG TCACCTCATC CGAGGGCTCC 
AGTGGGAAGA GCAAGAAGAG ATCGTCACTC TTCTCCCCCC GCAGAAACAA GAAAGAGAAG 
AAGTCCAAAG GTGAGGGCCG GCCCCCGGAG AAGCCCAGCC CAAGCCTTCT GGAGGAAGCT 
GCTGCCAAGC CCAAGTCCCT GTGGAAGTCG GTCTTTTCCG GGTACAAGAA GGACAAAAAG 
AAGAAGGGTG ATGACAAGTC CTGTTCCAGC ACCCCTTCCA GCGGTGCCAC TGTGGACTCT 
GGCAAGCACA AGATGTCCCC TGTTGTGAGA GCAGAGCTGC ACCTGCGGCG CCAGTTGAGC 
TTCTCCGAGG ACTCAGACCT GTCCAGTGAT GACATCCTTG AGAGGTCCTC CCAGAAGTCC 
AGGCGAGAGT CGGTTTATGT ACCACACGCC TTGGCATTCA AGAGATCATA TCCATCAAAG 
CCGAGAACCT ACACAGAGGA GGAACTGAAT GCCAAGCTGA CCCGGCGTGT GCAGAAGGCA 
GCTCGTAGAC AGGCCAAGCA AGAGGAGCTG AAGCGGCTGC ACCGAGCACA GATCATCCAG 
AGGCAGCTGG AGCAGGTGGA GGAGAAGCAG AGACAGCTGG AGGAAAGAGG GGTGGCCGTG 
GAGAAGGCGC TCCGTGGTGA AGCAGACTAT TGGGGAGAGT CTTATTACAG TGACCTCATC 
GACTTGCATC TGGGTGTTGA GCCCAGTGGC GGGACTCCAC GGCGTAGACC TCTCTCCTTC 
TGCCCTTGCT GTGTCCAGGA AGGCATGGGC AAGAAGGATG ACCCCAAGCT GATGCAGGAG 
TGGTTCAAGT TGGTGCAAGA GAAGAATGCC ATGGTGCGCT ATGAGTCGGA GCTGATGATT 
TTTGCCCGGG AGCTGGAACT GGAGGACCGG CAGAGCCGAC TGCAGCAGGA GCTCCGGGAG 
CGGATGGCGG TGGAAGATCA CCTGAAGACT GAGGAGGAGC TGTCTGAGGA GAAGAAGATC 
TTGAACGAGA TGCTGGAGGT GGTGGAGCAG AGAGATGCCC TCGTGGCCCT GCTTGAGGAG 
CAGCGACTCC GGGAGAAGGA GGAGGACAAA GACCTGGAGG CTGTCATGCT GTCCAAGGGC 
TTCAGCCTGA ACTGGTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_022410886.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022266594.1 Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X17, mRNA.

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CloneID	OCe282682
Clone ID Related Accession (Same CDS sequence)	XM_022410886.1
Accession Version	XM_022410886.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	F-actin-methionine sulfoxide oxidase MICAL3 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GGGCTCGATA AAGAAGGAGT TCCCACAGAA CTTAGGAGGC 
AGCGACACGT GCTATTTCTG CCGGAAGCGG GTCTACGTGA TGGAAAGGCT GAGTGCTGAG 
GGCAAGTTCT TCCATCGGAG CTGCTTCAAG TGCGAGTACT GCGCCACCAC CCTGCGCCTC 
TCGGCCTATG CCTATGACAT TGAGGACGGT AAATTCTACT GCAAACCACA CTACTGTTAT 
CGACTCTCTG GCTCTGCACA GAGGAAGAGA CCAGCAGTGG CTCCTCTCTC TGGAAAGGAG 
GCCAGAGAAC CCCTGCAGGA TGGCCCCACT GTGGACACAA ATGGACGGGC CAGCGCTGTC 
ACCAGCCCTG CTGAGAGAAC TCCAGGTTCA AGCGTGAATG GTGTGGAAGA GCCTAGCATT 
GCCAAGAGGC TCAGGGGCAC TCCCGAGCGG ATTGAGCTGG AGAACTACCG CCTGTCCGTG 
AAGCAGGCCG AGGAGCTGGA GGAGGTGCCC GAGGAGACAC AGGCCGAGCA CAACCTGAGC 
AGCGTGCTGG ACACGGGGGC AGAGGAGGAC GTGGCCAGCA GCAGTTCTGA GTCAGAGATG 
GAGGAAGAGG AGGAAGAAGA GCCTCCTCTG CCCACTTCGG ACCTGGGCGG TGTTCCTTGG 
AAGGAGGCCG TGAGGATCCA TGCTCTTCTG AAAGGAAAGA GTGAAGAGGA GCTAGAGGCC 
TCAAAGAGCT TTGGGCCTGG GCATGAAGAA GACGAGGAGG AGGAGGAGTA TGAAGAGGAG 
GAGGAGGAGG AGGAGGAAGA ATATGAAGAG GAAGAGGAAG AGGAAGAGTC TAGTGAAGAA 
GGGGAGTATT GCCCCTGGGA CAGAGAACTC CTACAAGGCC AGTGGCTCCG GCAGCTGTCA 
CAGGAAGAAA CTACGGGTAC ACGTAAAGTT GGGAGCCAGA GGCTACAGCA GATCGTAAAT 
CCGGCCGACC CCTTGGAGAT CCAGGCTGAT GTGCACTGGA CACATATCCG AGAGAGAGAG 
GAGGAGGAGC GAATGGTCCC AACCTCTGAG TCCTCCACTT CTAGAGTGCC TGACCTTCCC 
TCCATACGCC GCGCAGCAGT GCAGGCCTGG CTGGAGACGG TCTCTGGAGT CCCATTTGAT 
GAGAATGACC TGGAGGAAGA CGTGGACTCG GAGCCAGCAA AGATAGAAGG GGAGGCCACA 
GAGAATGGGG ACACAGGGGA CACTGGTGCT GAGCTGGACG ATGATCAGCG CTGGTCTGAC 
GGGATCCCAT CGGATGCCGA GACGGATCTC CGCGTGCAGC ACCAGGAGGC GGTAGAGGCA 
GACCTGGAGC TGAGGGTCTC AGAGAATGAG GAGGAGCCAC CTGAGGTCAC ACCAGGGCAT 
CCGGAGAGAA GTCTCACCCG GGTCCCCAGC CCTGCCCTGT CCCCTGAGGA GCAAGCTGGT 
CTGCGGTCCC CAGGCCACAG CCCTAAGGCA GAGGGGGCCC AAATCCCACT TGTCTCTGTT 
GTCACCAAGG CAAAATTGCC TGAAGAGAAC CTTTTCCCCA AGCCTTTGCT CCCCAAAGTG 
AAGCCCAAGG TGGAAGTGCC CTGCAGTCAG AGAGCTGCAT GCTCCCCCAT CCGTTCTCAG 
CCAGTGGCTC TGCCAGAAGC CAGGACACCT ACCTCCCCAG TGAGCTCGCA GTGCCTGTCG 
CCTTTGGCCA CCTCCACCCC CACCAGCACC CAGCTCCCCA TTTGCTCCCA GCCTCAGCCT 
TCCTCTGAGA CCACGGCCCC ATCCCCCACC AAGTCCCCCA TACGGCTCCA GCCTGTTTCA 
GCCAAAACAT CTATCCCAGT GGTTCCCCTC CCCTTGAAGA ACCAAGGGGA CACCAAGGAC 
AGGCTGGGCA GCCCTCTCGC TGTGGATGAG GCCCTGAAGC GGAATGACCT GGTGGCAGAG 
TTCTGGATGA AGAGCGCCGA GATCCGCCGC AGCCTCGGGC TCACACCAGT GGATCGGAGC 
AAGGTGTATG AGCCCAGCTT CCCTTCGCCT GCCTTTAAAC CAGTGTCCCT AAAATCCTAT 
GCAGTGGAGA AGTCTCCCGA GGGGGAGGGA CTCCAGCTTC TGAAGCCTCC ACCTGTTCCT 
AAGAGACTAG GCCTGCCGAA GTCAGACGGT GACCAGCCCT TCCTGCCAAC CCCCAAGTCC 
CCGTCGGACA GAGAGCTGAA AAGCTCCCAG GAGGAGAGGA GAGATCTGTC GAGCAGCTCC 
GGGCTGGGCC TTCACGGGAG CTCCTCCAAC ATGAAGACTT TGGGCAGCCA GAGCTTCAAC 
ACATCAGACT CCACCATGCT CACTCCCCCT TCAAGCCCAC CGCCACCACC ACCCCAAGAC 
GAGGAACCCG CAACTCTTCG GAGGAAGCCA TATCAGACTC ATGAGCATAA GGACGCTGAG 
CCCAAGGCCT CGGTGATTCC ACCACCACCA CCTGCGACGT TCATGCGGGC CCCCCGGGAG 
CCCACCCAGC CCCTCCAAGA GGAGATGCGG AAGTCGTTTG TGGAGAGCGT GGATGAGATC 
CCATTTGCCG ATGATGTGGA GGACACCTAT GATGACAAGA CCGAGGACTC GAGTCTCCAG 
GAGAAGTTCT TCACACCCCC CTCCTACTGG CCCCGCACGG AGAGGCCCCT CCACCCACCC 
CTAGCCAAGG AGAATGGTAG GTTGCCTACT CTGGAGAGTG GGGTCCAGCC ACAGAAGAGG 
GGCCTGCCCT TAGTCTCTCC AGAAGCCAAG GAGCTGGCTG AGGAGCGCAT GCGAGCCAGG 
GAGAAGTCTG TGAAGAGCCA GGCGTTGAGA GACGCTATGG CCAAGCAGCT GAGTAAGATG 
AAGGAAATGG AGATGGCGGC TGGGACCTCC AGTCCCCCAG GAGGCACCTC CCACAAAGCC 
TCCTCTGTTC CCTCCAAGGG CAAAGACCTC AGCCCCGAGT CCCCTAGACG CCCAGTTCTC 
AAAGGCCCCA GGGAGCCTGC CCTGAAGCAC GAAGCCACTA GTGAGGAGGT CCTCTCCCCA 
CCATCGGACT CAGGGGGCCC AGATGGTTCG GTCACCTCAT CCGAGGGCTC CAGTGGGAAG 
AGCAAGAAGA GATCGTCACT CTTCTCCCCC CGCAGAAACA AGAAAGAGAA GAAGTCCAAA 
GGTGAGGGCC GGCCCCCGGA GAAGCCCAGC CCAAGCCTTC TGGAGGAAGC TGCTGCCAAG 
CCCAAGTCCC TGTGGAAGTC GGTCTTTTCC GGGTACAAGA AGGACAAAAA GAAGAAGGGT 
GATGACAAGT CCTGTTCCAG CACCCCTTCC AGCGGTGCCA CTGTGGACTC TGGCAAGCAC 
AAGATGTCCC CTGTTGTGAG AGCAGAGCTG CACCTGCGGC GCCAGTTGAG CTTCTCCGAG 
GACTCAGACC TGTCCAGTGA TGACATCCTT GAGAGGTCCT CCCAGAAGTC CAGGCGAGAG 
TCGGTTTATG TACCACACGC CTTGGCATTC AAGAGATCAT ATCCATCAAA GCCGAGAACC 
TACACAGAGG AGGAACTGAA TGCCAAGCTG ACCCGGCGTG TGCAGAAGGC AGCTCGTAGA 
CAGGCCAAGC AAGAGGAGCT GAAGCGGCTG CACCGAGCAC AGATCATCCA GAGGCAGCTG 
GAGCAGGTGG AGGAGAAGCA GAGACAGCTG GAGGAAAGAG GGGTGGCCGT GGAGAAGGCG 
CTCCGTGGTG AAGCAGACTA TTGGGGAGAG TCTTATTACA GTGACCTCAT CGACTTGCAT 
CTGGGTGTTG AGCCCAGTGG CGGGACTCCA CGGCGTAGAC CTCTCTCCTT CTGCCCTTGC 
TGTGTCCAGG AAGGCATGGG CAAGAAGGAT GACCCCAAGC TGATGCAGGA GTGGTTCAAG 
TTGGTGCAAG AGAAGAATGC CATGGTGCGC TATGAGTCGG AGCTGATGAT TTTTGCCCGG 
GAGCTGGAAC TGGAGGACCG GCAGAGCCGA CTGCAGCAGG AGCTCCGGGA GCGGATGGCG 
GTGGAAGATC ACCTGAAGAC TGAGGAGGAG CTGTCTGAGG AGAAGAAGAT CTTGAACGAG 
ATGCTGGAGG TGGTGGAGCA GAGAGATGCC CTCGTGGCCC TGCTTGAGGA GCAGCGACTC 
CGGGAGAAGG AGGAGGACAA AGACCTGGAG GCTGTCATGC TGTCCAAGGG CTTCAGCCTG 
AACTGGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022266594.1
CDS	258..6869
Translation	MEDSKNETVNHAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDYRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKACTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFQGLVQPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELKEATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLNYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREQNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNININFLRPSQVRHLYNTGETRDIHLEMENLVNSRTTPKLTRNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGKEMASVGEPDKLSMVMYLTQFYEMFKDSLPSSDALDLNAEEKAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEEAPRGYRGGRPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSTGMRRQPTQERGVSQPSCCLPEQGHPAPTPQWKQQQEKERSRTCPKKVITLSPPLTPPPCRAPRSQQTYRDLDADSRGKQSPHHERGSIKKEFPQNLGGSDTCYFCRKRVYVMERLSAEGKFFHRSCFKCEYCATTLRLSAYAYDIEDGKFYCKPHYCYRLSGSAQRKRPAVAPLSGKEAREPLQDGPTVDTNGRASAVTSPAERTPGSSVNGVEEPSIAKRLRGTPERIELENYRLSVKQAEELEEVPEETQAEHNLSSVLDTGAEEDVASSSSESEMEEEEEEEPPLPTSDLGGVPWKEAVRIHALLKGKSEEELEASKSFGPGHEEDEEEEEYEEEEEEEEEEYEEEEEEEESSEEGEYCPWDRELLQGQWLRQLSQEETTGTRKVGSQRLQQIVNPADPLEIQADVHWTHIREREEEERMVPTSESSTSRVPDLPSIRRAAVQAWLETVSGVPFDENDLEEDVDSEPAKIEGEATENGDTGDTGAELDDDQRWSDGIPSDAETDLRVQHQEAVEADLELRVSENEEEPPEVTPGHPERSLTRVPSPALSPEEQAGLRSPGHSPKAEGAQIPLVSVVTKAKLPEENLFPKPLLPKVKPKVEVPCSQRAACSPIRSQPVALPEARTPTSPVSSQCLSPLATSTPTSTQLPICSQPQPSSETTAPSPTKSPIRLQPVSAKTSIPVVPLPLKNQGDTKDRLGSPLAVDEALKRNDLVAEFWMKSAEIRRSLGLTPVDRSKVYEPSFPSPAFKPVSLKSYAVEKSPEGEGLQLLKPPPVPKRLGLPKSDGDQPFLPTPKSPSDRELKSSQEERRDLSSSSGLGLHGSSSNMKTLGSQSFNTSDSTMLTPPSSPPPPPPQDEEPATLRRKPYQTHEHKDAEPKASVIPPPPPATFMRAPREPTQPLQEEMRKSFVESVDEIPFADDVEDTYDDKTEDSSLQEKFFTPPSYWPRTERPLHPPLAKENGRLPTLESGVQPQKRGLPLVSPEAKELAEERMRAREKSVKSQALRDAMAKQLSKMKEMEMAAGTSSPPGGTSHKASSVPSKGKDLSPESPRRPVLKGPREPALKHEATSEEVLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRNKKEKKSKGEGRPPEKPSPSLLEEAAAKPKSLWKSVFSGYKKDKKKKGDDKSCSSTPSSGATVDSGKHKMSPVVRAELHLRRQLSFSEDSDLSSDDILERSSQKSRRESVYVPHALAFKRSYPSKPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELSEEKKILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAVMLSKGFSLNWS

Target ORF information:

RefSeq Version	XM_022410886.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022410886.1

ORF Insert Sequence:

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GGGCTCGATA AAGAAGGAGT TCCCACAGAA CTTAGGAGGC 
AGCGACACGT GCTATTTCTG CCGGAAGCGG GTCTACGTGA TGGAAAGGCT GAGTGCTGAG 
GGCAAGTTCT TCCATCGGAG CTGCTTCAAG TGCGAGTACT GCGCCACCAC CCTGCGCCTC 
TCGGCCTATG CCTATGACAT TGAGGACGGT AAATTCTACT GCAAACCACA CTACTGTTAT 
CGACTCTCTG GCTCTGCACA GAGGAAGAGA CCAGCAGTGG CTCCTCTCTC TGGAAAGGAG 
GCCAGAGAAC CCCTGCAGGA TGGCCCCACT GTGGACACAA ATGGACGGGC CAGCGCTGTC 
ACCAGCCCTG CTGAGAGAAC TCCAGGTTCA AGCGTGAATG GTGTGGAAGA GCCTAGCATT 
GCCAAGAGGC TCAGGGGCAC TCCCGAGCGG ATTGAGCTGG AGAACTACCG CCTGTCCGTG 
AAGCAGGCCG AGGAGCTGGA GGAGGTGCCC GAGGAGACAC AGGCCGAGCA CAACCTGAGC 
AGCGTGCTGG ACACGGGGGC AGAGGAGGAC GTGGCCAGCA GCAGTTCTGA GTCAGAGATG 
GAGGAAGAGG AGGAAGAAGA GCCTCCTCTG CCCACTTCGG ACCTGGGCGG TGTTCCTTGG 
AAGGAGGCCG TGAGGATCCA TGCTCTTCTG AAAGGAAAGA GTGAAGAGGA GCTAGAGGCC 
TCAAAGAGCT TTGGGCCTGG GCATGAAGAA GACGAGGAGG AGGAGGAGTA TGAAGAGGAG 
GAGGAGGAGG AGGAGGAAGA ATATGAAGAG GAAGAGGAAG AGGAAGAGTC TAGTGAAGAA 
GGGGAGTATT GCCCCTGGGA CAGAGAACTC CTACAAGGCC AGTGGCTCCG GCAGCTGTCA 
CAGGAAGAAA CTACGGGTAC ACGTAAAGTT GGGAGCCAGA GGCTACAGCA GATCGTAAAT 
CCGGCCGACC CCTTGGAGAT CCAGGCTGAT GTGCACTGGA CACATATCCG AGAGAGAGAG 
GAGGAGGAGC GAATGGTCCC AACCTCTGAG TCCTCCACTT CTAGAGTGCC TGACCTTCCC 
TCCATACGCC GCGCAGCAGT GCAGGCCTGG CTGGAGACGG TCTCTGGAGT CCCATTTGAT 
GAGAATGACC TGGAGGAAGA CGTGGACTCG GAGCCAGCAA AGATAGAAGG GGAGGCCACA 
GAGAATGGGG ACACAGGGGA CACTGGTGCT GAGCTGGACG ATGATCAGCG CTGGTCTGAC 
GGGATCCCAT CGGATGCCGA GACGGATCTC CGCGTGCAGC ACCAGGAGGC GGTAGAGGCA 
GACCTGGAGC TGAGGGTCTC AGAGAATGAG GAGGAGCCAC CTGAGGTCAC ACCAGGGCAT 
CCGGAGAGAA GTCTCACCCG GGTCCCCAGC CCTGCCCTGT CCCCTGAGGA GCAAGCTGGT 
CTGCGGTCCC CAGGCCACAG CCCTAAGGCA GAGGGGGCCC AAATCCCACT TGTCTCTGTT 
GTCACCAAGG CAAAATTGCC TGAAGAGAAC CTTTTCCCCA AGCCTTTGCT CCCCAAAGTG 
AAGCCCAAGG TGGAAGTGCC CTGCAGTCAG AGAGCTGCAT GCTCCCCCAT CCGTTCTCAG 
CCAGTGGCTC TGCCAGAAGC CAGGACACCT ACCTCCCCAG TGAGCTCGCA GTGCCTGTCG 
CCTTTGGCCA CCTCCACCCC CACCAGCACC CAGCTCCCCA TTTGCTCCCA GCCTCAGCCT 
TCCTCTGAGA CCACGGCCCC ATCCCCCACC AAGTCCCCCA TACGGCTCCA GCCTGTTTCA 
GCCAAAACAT CTATCCCAGT GGTTCCCCTC CCCTTGAAGA ACCAAGGGGA CACCAAGGAC 
AGGCTGGGCA GCCCTCTCGC TGTGGATGAG GCCCTGAAGC GGAATGACCT GGTGGCAGAG 
TTCTGGATGA AGAGCGCCGA GATCCGCCGC AGCCTCGGGC TCACACCAGT GGATCGGAGC 
AAGGTGTATG AGCCCAGCTT CCCTTCGCCT GCCTTTAAAC CAGTGTCCCT AAAATCCTAT 
GCAGTGGAGA AGTCTCCCGA GGGGGAGGGA CTCCAGCTTC TGAAGCCTCC ACCTGTTCCT 
AAGAGACTAG GCCTGCCGAA GTCAGACGGT GACCAGCCCT TCCTGCCAAC CCCCAAGTCC 
CCGTCGGACA GAGAGCTGAA AAGCTCCCAG GAGGAGAGGA GAGATCTGTC GAGCAGCTCC 
GGGCTGGGCC TTCACGGGAG CTCCTCCAAC ATGAAGACTT TGGGCAGCCA GAGCTTCAAC 
ACATCAGACT CCACCATGCT CACTCCCCCT TCAAGCCCAC CGCCACCACC ACCCCAAGAC 
GAGGAACCCG CAACTCTTCG GAGGAAGCCA TATCAGACTC ATGAGCATAA GGACGCTGAG 
CCCAAGGCCT CGGTGATTCC ACCACCACCA CCTGCGACGT TCATGCGGGC CCCCCGGGAG 
CCCACCCAGC CCCTCCAAGA GGAGATGCGG AAGTCGTTTG TGGAGAGCGT GGATGAGATC 
CCATTTGCCG ATGATGTGGA GGACACCTAT GATGACAAGA CCGAGGACTC GAGTCTCCAG 
GAGAAGTTCT TCACACCCCC CTCCTACTGG CCCCGCACGG AGAGGCCCCT CCACCCACCC 
CTAGCCAAGG AGAATGGTAG GTTGCCTACT CTGGAGAGTG GGGTCCAGCC ACAGAAGAGG 
GGCCTGCCCT TAGTCTCTCC AGAAGCCAAG GAGCTGGCTG AGGAGCGCAT GCGAGCCAGG 
GAGAAGTCTG TGAAGAGCCA GGCGTTGAGA GACGCTATGG CCAAGCAGCT GAGTAAGATG 
AAGGAAATGG AGATGGCGGC TGGGACCTCC AGTCCCCCAG GAGGCACCTC CCACAAAGCC 
TCCTCTGTTC CCTCCAAGGG CAAAGACCTC AGCCCCGAGT CCCCTAGACG CCCAGTTCTC 
AAAGGCCCCA GGGAGCCTGC CCTGAAGCAC GAAGCCACTA GTGAGGAGGT CCTCTCCCCA 
CCATCGGACT CAGGGGGCCC AGATGGTTCG GTCACCTCAT CCGAGGGCTC CAGTGGGAAG 
AGCAAGAAGA GATCGTCACT CTTCTCCCCC CGCAGAAACA AGAAAGAGAA GAAGTCCAAA 
GGTGAGGGCC GGCCCCCGGA GAAGCCCAGC CCAAGCCTTC TGGAGGAAGC TGCTGCCAAG 
CCCAAGTCCC TGTGGAAGTC GGTCTTTTCC GGGTACAAGA AGGACAAAAA GAAGAAGGGT 
GATGACAAGT CCTGTTCCAG CACCCCTTCC AGCGGTGCCA CTGTGGACTC TGGCAAGCAC 
AAGATGTCCC CTGTTGTGAG AGCAGAGCTG CACCTGCGGC GCCAGTTGAG CTTCTCCGAG 
GACTCAGACC TGTCCAGTGA TGACATCCTT GAGAGGTCCT CCCAGAAGTC CAGGCGAGAG 
TCGGTTTATG TACCACACGC CTTGGCATTC AAGAGATCAT ATCCATCAAA GCCGAGAACC 
TACACAGAGG AGGAACTGAA TGCCAAGCTG ACCCGGCGTG TGCAGAAGGC AGCTCGTAGA 
CAGGCCAAGC AAGAGGAGCT GAAGCGGCTG CACCGAGCAC AGATCATCCA GAGGCAGCTG 
GAGCAGGTGG AGGAGAAGCA GAGACAGCTG GAGGAAAGAG GGGTGGCCGT GGAGAAGGCG 
CTCCGTGGTG AAGCAGACTA TTGGGGAGAG TCTTATTACA GTGACCTCAT CGACTTGCAT 
CTGGGTGTTG AGCCCAGTGG CGGGACTCCA CGGCGTAGAC CTCTCTCCTT CTGCCCTTGC 
TGTGTCCAGG AAGGCATGGG CAAGAAGGAT GACCCCAAGC TGATGCAGGA GTGGTTCAAG 
TTGGTGCAAG AGAAGAATGC CATGGTGCGC TATGAGTCGG AGCTGATGAT TTTTGCCCGG 
GAGCTGGAAC TGGAGGACCG GCAGAGCCGA CTGCAGCAGG AGCTCCGGGA GCGGATGGCG 
GTGGAAGATC ACCTGAAGAC TGAGGAGGAG CTGTCTGAGG AGAAGAAGAT CTTGAACGAG 
ATGCTGGAGG TGGTGGAGCA GAGAGATGCC CTCGTGGCCC TGCTTGAGGA GCAGCGACTC 
CGGGAGAAGG AGGAGGACAA AGACCTGGAG GCTGTCATGC TGTCCAAGGG CTTCAGCCTG 
AACTGGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_022410887.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022266595.1 Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X18, mRNA.

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CloneID	OCe282683
Clone ID Related Accession (Same CDS sequence)	XM_022410887.1
Accession Version	XM_022410887.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	F-actin-methionine sulfoxide oxidase MICAL3 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGTGCCTG ACCTTCCCTC CATACGCCGC 
GCAGCAGTGC AGGCCTGGCT GGAGACGGTC TCTGGAGTCC CATTTGATGA GAATGACCTG 
GAGGAAGACG TGGACTCGGA GCCAGCAAAG ATAGAAGGGG AGGCCACAGA GAATGGGGAC 
ACAGGGGACA CTGGTGCTGA GCTGGACGAT GATCAGCGCT GGTCTGACGG GATCCCATCG 
GATGCCGAGA CGGATCTCCG CGTGCAGCAC CAGGAGGCGG TAGAGGCAGA CCTGGAGCTG 
AGGGTCTCAG AGAATGAGGA GGAGCCACCT GAGGTCACAC CAGGGCATCC GGAGAGAAGT 
CTCACCCGGG TCCCCAGCCC TGCCCTGTCC CCTGAGGAGC AAGCTGGTCT GCGGTCCCCA 
GGCCACAGCC CTAAGGCAGA GGGGGCCCAA ATCCCACTTG TCTCTGTTGT CACCAAGGCA 
AAATTGCCTG AAGAGAACCT TTTCCCCAAG CCTTTGCTCC CCAAAGTGAA GCCCAAGGTG 
GAAGTGCCCT GCAGTCAGAG AGCTGCATGC TCCCCCATCC GTTCTCAGCC AGTGGCTCTG 
CCAGAAGCCA GGACACCTAC CTCCCCAGTG AGCTCGCAGT GCCTGTCGCC TTTGGCCACC 
TCCACCCCCA CCAGCACCCA GCTCCCCATT TGCTCCCAGC CTCAGCCTTC CTCTGAGACC 
ACGGCCCCAT CCCCCACCAA GTCCCCCATA CGGCTCCAGC CTGTTTCAGC CAAAACATCT 
ATCCCAGTGG TTCCCCTCCC CTTGAAGAAC CAAGGGGACA CCAAGGACAG GCTGGGCAGC 
CCTCTCGCTG TGGATGAGGC CCTGAAGCGG AATGACCTGG TGGCAGAGTT CTGGATGAAG 
AGCGCCGAGA TCCGCCGCAG CCTCGGGCTC ACACCAGTGG ATCGGAGCAA GGTGTATGAG 
CCCAGCTTCC CTTCGCCTGC CTTTAAACCA GTGTCCCTAA AATCCTATGC AGTGGAGAAG 
TCTCCCGAGG GGGAGGGACT CCAGCTTCTG AAGCCTCCAC CTGTTCCTAA GAGACTAGGC 
CTGCCGAAGT CAGACGGTGA CCAGCCCTTC CTGCCAACCC CCAAGTCCCC GTCGGACAGA 
GAGCTGAAAA GCTCCCAGGA GGAGAGGAGA GATCTGTCGA GCAGCTCCGG GCTGGGCCTT 
CACGGGAGCT CCTCCAACAT GAAGACTTTG GGCAGCCAGA GCTTCAACAC ATCAGACTCC 
ACCATGCTCA CTCCCCCTTC AAGCCCACCG CCACCACCAC CCCAAGACGA GGAACCCGCA 
ACTCTTCGGA GGAAGCCATA TCAGACTCAT GAGCATAAGG ACGCTGAGCC CAAGGCCTCG 
GTGATTCCAC CACCACCACC TGCGACGTTC ATGCGGGCCC CCCGGGAGCC CACCCAGCCC 
CTCCAAGAGG AGATGCGGAA GTCGTTTGTG GAGAGCGTGG ATGAGATCCC ATTTGCCGAT 
GATGTGGAGG ACACCTATGA TGACAAGACC GAGGACTCGA GTCTCCAGGA GAAGTTCTTC 
ACACCCCCCT CCTACTGGCC CCGCACGGAG AGGCCCCTCC ACCCACCCCT AGCCAAGGAG 
AATGGTAGGT TGCCTACTCT GGAGAGTGGG GTCCAGCCAC AGAAGAGGGG CCTGCCCTTA 
GTCTCTCCAG AAGCCAAGGA GCTGGCTGAG GAGCGCATGC GAGCCAGGGA GAAGTCTGTG 
AAGAGCCAGG CGTTGAGAGA CGCTATGGCC AAGCAGCTGA GTAAGATGAA GGAAATGGAG 
ATGGCGGCTG GGACCTCCAG TCCCCCAGGA GGCACCTCCC ACAAAGCCTC CTCTGTTCCC 
TCCAAGGGCA AAGACCTCAG CCCCGAGTCC CCTAGACGCC CAGTTCTCAA AGGCCCCAGG 
GAGCCTGCCC TGAAGCACGA AGCCACTAGT GAGGAGGTCC TCTCCCCACC ATCGGACTCA 
GGGGGCCCAG ATGGTTCGGT CACCTCATCC GAGGGCTCCA GTGGGAAGAG CAAGAAGAGA 
TCGTCACTCT TCTCCCCCCG CAGAAACAAG AAAGAGAAGA AGTCCAAAGG TGAGGGCCGG 
CCCCCGGAGA AGCCCAGCCC AAGCCTTCTG GAGGAAGCTG CTGCCAAGCC CAAGTCCCTG 
TGGAAGTCGG TCTTTTCCGG GTACAAGAAG GACAAAAAGA AGAAGGGTGA TGACAAGTCC 
TGTTCCAGCA CCCCTTCCAG CGGTGCCACT GTGGACTCTG GCAAGCACAA GATGTCCCCT 
GTTGTGAGAG CAGAGCTGCA CCTGCGGCGC CAGTTGAGCT TCTCCGAGGA CTCAGACCTG 
TCCAGTGATG ACATCCTTGA GAGGTCCTCC CAGAAGTCCA GGCGAGAGTC GGTTTATGTA 
CCACACGCCT TGGCATTCAA GAGATCATAT CCATCAAAGC CGAGAACCTA CACAGAGGAG 
GAACTGAATG CCAAGCTGAC CCGGCGTGTG CAGAAGGCAG CTCGTAGACA GGCCAAGCAA 
GAGGAGCTGA AGCGGCTGCA CCGAGCACAG ATCATCCAGA GGCAGCTGGA GCAGGTGGAG 
GAGAAGCAGA GACAGCTGGA GGAAAGAGGG GTGGCCGTGG AGAAGGCGCT CCGTGGTGAA 
GCAGACTATT GGGGAGAGTC TTATTACAGT GACCTCATCG ACTTGCATCT GGGTGTTGAG 
CCCAGTGGCG GGACTCCACG GCGTAGACCT CTCTCCTTCT GCCCTTGCTG TGTCCAGGAA 
GGCATGGGCA AGAAGGATGA CCCCAAGCTG ATGCAGGAGT GGTTCAAGTT GGTGCAAGAG 
AAGAATGCCA TGGTGCGCTA TGAGTCGGAG CTGATGATTT TTGCCCGGGA GCTGGAACTG 
GAGGACCGGC AGAGCCGACT GCAGCAGGAG CTCCGGGAGC GGATGGCGGT GGAAGATCAC 
CTGAAGACTG AGGAGGAGCT GTCTGAGGAG AAGAAGATCT TGAACGAGAT GCTGGAGGTG 
GTGGAGCAGA GAGATGCCCT CGTGGCCCTG CTTGAGGAGC AGCGACTCCG GGAGAAGGAG 
GAGGACAAAG ACCTGGAGGC TGTCATGCTG TCCAAGGGCT TCAGCCTGAA CTGGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022266595.1
CDS	258..6857
Translation	MEDSKNETVNHAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDYRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKACTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFQGLVQPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELKEATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLNYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREQNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNININFLRPSQVRHLYNTGETRDIHLEMENLVNSRTTPKLTRNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGKEMASVGEPDKLSMVMYLTQFYEMFKDSLPSSDALDLNAEEKAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEEAPRGYRGGRPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSTGMRRQPTQERGVSQPSCCLPEQGHPAPTPQWKQQQEKERSRTCPKKVITLSPPLTPPPCRAPRSQQTYRDLDADSRGKQSPHHERPEPEPSRRFFVDQWELSLSLRSSNRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCRKRVYVMERLSAEGKFFHRSCFKCEYCATTLRLSAYAYDIEDGKFYCKPHYCYRLSGSAQRKRPAVAPLSGKEAREPLQDGPTVDTNGRASAVTSPAERTPGSSVNGVEEPSIAKRLRGTPERIELENYRLSVKQAEELEEVPEETQAEHNLSSVLDTGAEEDVASSSSESEMEEEEEEEPPLPTSDLGGVPWKEAVRIHALLKGKSEEELEASKSFGPGHEEDEEEEEYEEEEEEEEEEYEEEEEEEESSEVPDLPSIRRAAVQAWLETVSGVPFDENDLEEDVDSEPAKIEGEATENGDTGDTGAELDDDQRWSDGIPSDAETDLRVQHQEAVEADLELRVSENEEEPPEVTPGHPERSLTRVPSPALSPEEQAGLRSPGHSPKAEGAQIPLVSVVTKAKLPEENLFPKPLLPKVKPKVEVPCSQRAACSPIRSQPVALPEARTPTSPVSSQCLSPLATSTPTSTQLPICSQPQPSSETTAPSPTKSPIRLQPVSAKTSIPVVPLPLKNQGDTKDRLGSPLAVDEALKRNDLVAEFWMKSAEIRRSLGLTPVDRSKVYEPSFPSPAFKPVSLKSYAVEKSPEGEGLQLLKPPPVPKRLGLPKSDGDQPFLPTPKSPSDRELKSSQEERRDLSSSSGLGLHGSSSNMKTLGSQSFNTSDSTMLTPPSSPPPPPPQDEEPATLRRKPYQTHEHKDAEPKASVIPPPPPATFMRAPREPTQPLQEEMRKSFVESVDEIPFADDVEDTYDDKTEDSSLQEKFFTPPSYWPRTERPLHPPLAKENGRLPTLESGVQPQKRGLPLVSPEAKELAEERMRAREKSVKSQALRDAMAKQLSKMKEMEMAAGTSSPPGGTSHKASSVPSKGKDLSPESPRRPVLKGPREPALKHEATSEEVLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRNKKEKKSKGEGRPPEKPSPSLLEEAAAKPKSLWKSVFSGYKKDKKKKGDDKSCSSTPSSGATVDSGKHKMSPVVRAELHLRRQLSFSEDSDLSSDDILERSSQKSRRESVYVPHALAFKRSYPSKPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELSEEKKILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAVMLSKGFSLNWS

Target ORF information:

RefSeq Version	XM_022410887.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022410887.1

ORF Insert Sequence:

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGTGCCTG ACCTTCCCTC CATACGCCGC 
GCAGCAGTGC AGGCCTGGCT GGAGACGGTC TCTGGAGTCC CATTTGATGA GAATGACCTG 
GAGGAAGACG TGGACTCGGA GCCAGCAAAG ATAGAAGGGG AGGCCACAGA GAATGGGGAC 
ACAGGGGACA CTGGTGCTGA GCTGGACGAT GATCAGCGCT GGTCTGACGG GATCCCATCG 
GATGCCGAGA CGGATCTCCG CGTGCAGCAC CAGGAGGCGG TAGAGGCAGA CCTGGAGCTG 
AGGGTCTCAG AGAATGAGGA GGAGCCACCT GAGGTCACAC CAGGGCATCC GGAGAGAAGT 
CTCACCCGGG TCCCCAGCCC TGCCCTGTCC CCTGAGGAGC AAGCTGGTCT GCGGTCCCCA 
GGCCACAGCC CTAAGGCAGA GGGGGCCCAA ATCCCACTTG TCTCTGTTGT CACCAAGGCA 
AAATTGCCTG AAGAGAACCT TTTCCCCAAG CCTTTGCTCC CCAAAGTGAA GCCCAAGGTG 
GAAGTGCCCT GCAGTCAGAG AGCTGCATGC TCCCCCATCC GTTCTCAGCC AGTGGCTCTG 
CCAGAAGCCA GGACACCTAC CTCCCCAGTG AGCTCGCAGT GCCTGTCGCC TTTGGCCACC 
TCCACCCCCA CCAGCACCCA GCTCCCCATT TGCTCCCAGC CTCAGCCTTC CTCTGAGACC 
ACGGCCCCAT CCCCCACCAA GTCCCCCATA CGGCTCCAGC CTGTTTCAGC CAAAACATCT 
ATCCCAGTGG TTCCCCTCCC CTTGAAGAAC CAAGGGGACA CCAAGGACAG GCTGGGCAGC 
CCTCTCGCTG TGGATGAGGC CCTGAAGCGG AATGACCTGG TGGCAGAGTT CTGGATGAAG 
AGCGCCGAGA TCCGCCGCAG CCTCGGGCTC ACACCAGTGG ATCGGAGCAA GGTGTATGAG 
CCCAGCTTCC CTTCGCCTGC CTTTAAACCA GTGTCCCTAA AATCCTATGC AGTGGAGAAG 
TCTCCCGAGG GGGAGGGACT CCAGCTTCTG AAGCCTCCAC CTGTTCCTAA GAGACTAGGC 
CTGCCGAAGT CAGACGGTGA CCAGCCCTTC CTGCCAACCC CCAAGTCCCC GTCGGACAGA 
GAGCTGAAAA GCTCCCAGGA GGAGAGGAGA GATCTGTCGA GCAGCTCCGG GCTGGGCCTT 
CACGGGAGCT CCTCCAACAT GAAGACTTTG GGCAGCCAGA GCTTCAACAC ATCAGACTCC 
ACCATGCTCA CTCCCCCTTC AAGCCCACCG CCACCACCAC CCCAAGACGA GGAACCCGCA 
ACTCTTCGGA GGAAGCCATA TCAGACTCAT GAGCATAAGG ACGCTGAGCC CAAGGCCTCG 
GTGATTCCAC CACCACCACC TGCGACGTTC ATGCGGGCCC CCCGGGAGCC CACCCAGCCC 
CTCCAAGAGG AGATGCGGAA GTCGTTTGTG GAGAGCGTGG ATGAGATCCC ATTTGCCGAT 
GATGTGGAGG ACACCTATGA TGACAAGACC GAGGACTCGA GTCTCCAGGA GAAGTTCTTC 
ACACCCCCCT CCTACTGGCC CCGCACGGAG AGGCCCCTCC ACCCACCCCT AGCCAAGGAG 
AATGGTAGGT TGCCTACTCT GGAGAGTGGG GTCCAGCCAC AGAAGAGGGG CCTGCCCTTA 
GTCTCTCCAG AAGCCAAGGA GCTGGCTGAG GAGCGCATGC GAGCCAGGGA GAAGTCTGTG 
AAGAGCCAGG CGTTGAGAGA CGCTATGGCC AAGCAGCTGA GTAAGATGAA GGAAATGGAG 
ATGGCGGCTG GGACCTCCAG TCCCCCAGGA GGCACCTCCC ACAAAGCCTC CTCTGTTCCC 
TCCAAGGGCA AAGACCTCAG CCCCGAGTCC CCTAGACGCC CAGTTCTCAA AGGCCCCAGG 
GAGCCTGCCC TGAAGCACGA AGCCACTAGT GAGGAGGTCC TCTCCCCACC ATCGGACTCA 
GGGGGCCCAG ATGGTTCGGT CACCTCATCC GAGGGCTCCA GTGGGAAGAG CAAGAAGAGA 
TCGTCACTCT TCTCCCCCCG CAGAAACAAG AAAGAGAAGA AGTCCAAAGG TGAGGGCCGG 
CCCCCGGAGA AGCCCAGCCC AAGCCTTCTG GAGGAAGCTG CTGCCAAGCC CAAGTCCCTG 
TGGAAGTCGG TCTTTTCCGG GTACAAGAAG GACAAAAAGA AGAAGGGTGA TGACAAGTCC 
TGTTCCAGCA CCCCTTCCAG CGGTGCCACT GTGGACTCTG GCAAGCACAA GATGTCCCCT 
GTTGTGAGAG CAGAGCTGCA CCTGCGGCGC CAGTTGAGCT TCTCCGAGGA CTCAGACCTG 
TCCAGTGATG ACATCCTTGA GAGGTCCTCC CAGAAGTCCA GGCGAGAGTC GGTTTATGTA 
CCACACGCCT TGGCATTCAA GAGATCATAT CCATCAAAGC CGAGAACCTA CACAGAGGAG 
GAACTGAATG CCAAGCTGAC CCGGCGTGTG CAGAAGGCAG CTCGTAGACA GGCCAAGCAA 
GAGGAGCTGA AGCGGCTGCA CCGAGCACAG ATCATCCAGA GGCAGCTGGA GCAGGTGGAG 
GAGAAGCAGA GACAGCTGGA GGAAAGAGGG GTGGCCGTGG AGAAGGCGCT CCGTGGTGAA 
GCAGACTATT GGGGAGAGTC TTATTACAGT GACCTCATCG ACTTGCATCT GGGTGTTGAG 
CCCAGTGGCG GGACTCCACG GCGTAGACCT CTCTCCTTCT GCCCTTGCTG TGTCCAGGAA 
GGCATGGGCA AGAAGGATGA CCCCAAGCTG ATGCAGGAGT GGTTCAAGTT GGTGCAAGAG 
AAGAATGCCA TGGTGCGCTA TGAGTCGGAG CTGATGATTT TTGCCCGGGA GCTGGAACTG 
GAGGACCGGC AGAGCCGACT GCAGCAGGAG CTCCGGGAGC GGATGGCGGT GGAAGATCAC 
CTGAAGACTG AGGAGGAGCT GTCTGAGGAG AAGAAGATCT TGAACGAGAT GCTGGAGGTG 
GTGGAGCAGA GAGATGCCCT CGTGGCCCTG CTTGAGGAGC AGCGACTCCG GGAGAAGGAG 
GAGGACAAAG ACCTGGAGGC TGTCATGCTG TCCAAGGGCT TCAGCCTGAA CTGGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_022410888.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022266596.1 Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X19, mRNA.

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CloneID	OCe282684
Clone ID Related Accession (Same CDS sequence)	XM_022410888.1
Accession Version	XM_022410888.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	F-actin-methionine sulfoxide oxidase MICAL3 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGTCCCAT TTGATGAGAA TGACCTGGAG 
GAAGACGTGG ACTCGGAGCC AGCAAAGATA GAAGGGGAGG CCACAGAGAA TGGGGACACA 
GGGGACACTG GTGCTGAGCT GGACGATGAT CAGCGCTGGT CTGACGGGAT CCCATCGGAT 
GCCGAGACGG ATCTCCGCGT GCAGCACCAG GAGGCGGTAG AGGCAGACCT GGAGCTGAGG 
GTCTCAGAGA ATGAGGAGGA GCCACCTGAG GTCACACCAG GGCATCCGGA GAGAAGTCTC 
ACCCGGGTCC CCAGCCCTGC CCTGTCCCCT GAGGAGCAAG CTGGTCTGCG GTCCCCAGGC 
CACAGCCCTA AGGCAGAGGG GGCCCAAATC CCACTTGTCT CTGTTGTCAC CAAGGCAAAA 
TTGCCTGAAG AGAACCTTTT CCCCAAGCCT TTGCTCCCCA AAGTGAAGCC CAAGGTGGAA 
GTGCCCTGCA GTCAGAGAGC TGCATGCTCC CCCATCCGTT CTCAGCCAGT GGCTCTGCCA 
GAAGCCAGGA CACCTACCTC CCCAGTGAGC TCGCAGTGCC TGTCGCCTTT GGCCACCTCC 
ACCCCCACCA GCACCCAGCT CCCCATTTGC TCCCAGCCTC AGCCTTCCTC TGAGACCACG 
GCCCCATCCC CCACCAAGTC CCCCATACGG CTCCAGCCTG TTTCAGCCAA AACATCTATC 
CCAGTGGTTC CCCTCCCCTT GAAGAACCAA GGGGACACCA AGGACAGGCT GGGCAGCCCT 
CTCGCTGTGG ATGAGGCCCT GAAGCGGAAT GACCTGGTGG CAGAGTTCTG GATGAAGAGC 
GCCGAGATCC GCCGCAGCCT CGGGCTCACA CCAGTGGATC GGAGCAAGGT GTATGAGCCC 
AGCTTCCCTT CGCCTGCCTT TAAACCAGTG TCCCTAAAAT CCTATGCAGT GGAGAAGTCT 
CCCGAGGGGG AGGGACTCCA GCTTCTGAAG CCTCCACCTG TTCCTAAGAG ACTAGGCCTG 
CCGAAGTCAG ACGGTGACCA GCCCTTCCTG CCAACCCCCA AGTCCCCGTC GGACAGAGAG 
CTGAAAAGCT CCCAGGAGGA GAGGAGAGAT CTGTCGAGCA GCTCCGGGCT GGGCCTTCAC 
GGGAGCTCCT CCAACATGAA GACTTTGGGC AGCCAGAGCT TCAACACATC AGACTCCACC 
ATGCTCACTC CCCCTTCAAG CCCACCGCCA CCACCACCCC AAGACGAGGA ACCCGCAACT 
CTTCGGAGGA AGCCATATCA GACTCATGAG CATAAGGACG CTGAGCCCAA GGCCTCGGTG 
ATTCCACCAC CACCACCTGC GACGTTCATG CGGGCCCCCC GGGAGCCCAC CCAGCCCCTC 
CAAGAGGAGA TGCGGAAGTC GTTTGTGGAG AGCGTGGATG AGATCCCATT TGCCGATGAT 
GTGGAGGACA CCTATGATGA CAAGACCGAG GACTCGAGTC TCCAGGAGAA GTTCTTCACA 
CCCCCCTCCT ACTGGCCCCG CACGGAGAGG CCCCTCCACC CACCCCTAGC CAAGGAGAAT 
GGTAGGTTGC CTACTCTGGA GAGTGGGGTC CAGCCACAGA AGAGGGGCCT GCCCTTAGTC 
TCTCCAGAAG CCAAGGAGCT GGCTGAGGAG CGCATGCGAG CCAGGGAGAA GTCTGTGAAG 
AGCCAGGCGT TGAGAGACGC TATGGCCAAG CAGCTGAGTA AGATGAAGGA AATGGAGATG 
GCGGCTGGGA CCTCCAGTCC CCCAGGAGGC ACCTCCCACA AAGCCTCCTC TGTTCCCTCC 
AAGGGCAAAG ACCTCAGCCC CGAGTCCCCT AGACGCCCAG TTCTCAAAGG CCCCAGGGAG 
CCTGCCCTGA AGCACGAAGC CACTAGTGAG GAGGTCCTCT CCCCACCATC GGACTCAGGG 
GGCCCAGATG GTTCGGTCAC CTCATCCGAG GGCTCCAGTG GGAAGAGCAA GAAGAGATCG 
TCACTCTTCT CCCCCCGCAG AAACAAGAAA GAGAAGAAGT CCAAAGGTGA GGGCCGGCCC 
CCGGAGAAGC CCAGCCCAAG CCTTCTGGAG GAAGCTGCTG CCAAGCCCAA GTCCCTGTGG 
AAGTCGGTCT TTTCCGGGTA CAAGAAGGAC AAAAAGAAGA AGGGTGATGA CAAGTCCTGT 
TCCAGCACCC CTTCCAGCGG TGCCACTGTG GACTCTGGCA AGCACAAGAT GTCCCCTGTT 
GTGAGAGCAG AGCTGCACCT GCGGCGCCAG TTGAGCTTCT CCGAGGACTC AGACCTGTCC 
AGTGATGACA TCCTTGAGAG GTCCTCCCAG AAGTCCAGGC GAGAGTCGGT TTATGTACCA 
CACGCCTTGG CATTCAAGAG ATCATATCCA TCAAAGCCGA GAACCTACAC AGAGGAGGAA 
CTGAATGCCA AGCTGACCCG GCGTGTGCAG AAGGCAGCTC GTAGACAGGC CAAGCAAGAG 
GAGCTGAAGC GGCTGCACCG AGCACAGATC ATCCAGAGGC AGCTGGAGCA GGTGGAGGAG 
AAGCAGAGAC AGCTGGAGGA AAGAGGGGTG GCCGTGGAGA AGGCGCTCCG TGGTGAAGCA 
GACTATTGGG GAGAGTCTTA TTACAGTGAC CTCATCGACT TGCATCTGGG TGTTGAGCCC 
AGTGGCGGGA CTCCACGGCG TAGACCTCTC TCCTTCTGCC CTTGCTGTGT CCAGGAAGGC 
ATGGGCAAGA AGGATGACCC CAAGCTGATG CAGGAGTGGT TCAAGTTGGT GCAAGAGAAG 
AATGCCATGG TGCGCTATGA GTCGGAGCTG ATGATTTTTG CCCGGGAGCT GGAACTGGAG 
GACCGGCAGA GCCGACTGCA GCAGGAGCTC CGGGAGCGGA TGGCGGTGGA AGATCACCTG 
AAGACTGAGG AGGAGCTGTC TGAGGAGAAG AAGATCTTGA ACGAGATGCT GGAGGTGGTG 
GAGCAGAGAG ATGCCCTCGT GGCCCTGCTT GAGGAGCAGC GACTCCGGGA GAAGGAGGAG 
GACAAAGACC TGGAGGCTGT CATGCTGTCC AAGGGCTTCA GCCTGAACTG GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022266596.1
CDS	258..6794
Translation	MEDSKNETVNHAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDYRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKACTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFQGLVQPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELKEATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLNYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREQNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNININFLRPSQVRHLYNTGETRDIHLEMENLVNSRTTPKLTRNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGKEMASVGEPDKLSMVMYLTQFYEMFKDSLPSSDALDLNAEEKAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEEAPRGYRGGRPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSTGMRRQPTQERGVSQPSCCLPEQGHPAPTPQWKQQQEKERSRTCPKKVITLSPPLTPPPCRAPRSQQTYRDLDADSRGKQSPHHERPEPEPSRRFFVDQWELSLSLRSSNRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCRKRVYVMERLSAEGKFFHRSCFKCEYCATTLRLSAYAYDIEDGKFYCKPHYCYRLSGSAQRKRPAVAPLSGKEAREPLQDGPTVDTNGRASAVTSPAERTPGSSVNGVEEPSIAKRLRGTPERIELENYRLSVKQAEELEEVPEETQAEHNLSSVLDTGAEEDVASSSSESEMEEEEEEEPPLPTSDLGGVPWKEAVRIHALLKGKSEEELEASKSFGPGHEEDEEEEEYEEEEEEEEEEYEEEEEEEESSEVPFDENDLEEDVDSEPAKIEGEATENGDTGDTGAELDDDQRWSDGIPSDAETDLRVQHQEAVEADLELRVSENEEEPPEVTPGHPERSLTRVPSPALSPEEQAGLRSPGHSPKAEGAQIPLVSVVTKAKLPEENLFPKPLLPKVKPKVEVPCSQRAACSPIRSQPVALPEARTPTSPVSSQCLSPLATSTPTSTQLPICSQPQPSSETTAPSPTKSPIRLQPVSAKTSIPVVPLPLKNQGDTKDRLGSPLAVDEALKRNDLVAEFWMKSAEIRRSLGLTPVDRSKVYEPSFPSPAFKPVSLKSYAVEKSPEGEGLQLLKPPPVPKRLGLPKSDGDQPFLPTPKSPSDRELKSSQEERRDLSSSSGLGLHGSSSNMKTLGSQSFNTSDSTMLTPPSSPPPPPPQDEEPATLRRKPYQTHEHKDAEPKASVIPPPPPATFMRAPREPTQPLQEEMRKSFVESVDEIPFADDVEDTYDDKTEDSSLQEKFFTPPSYWPRTERPLHPPLAKENGRLPTLESGVQPQKRGLPLVSPEAKELAEERMRAREKSVKSQALRDAMAKQLSKMKEMEMAAGTSSPPGGTSHKASSVPSKGKDLSPESPRRPVLKGPREPALKHEATSEEVLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRNKKEKKSKGEGRPPEKPSPSLLEEAAAKPKSLWKSVFSGYKKDKKKKGDDKSCSSTPSSGATVDSGKHKMSPVVRAELHLRRQLSFSEDSDLSSDDILERSSQKSRRESVYVPHALAFKRSYPSKPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELSEEKKILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAVMLSKGFSLNWS

Target ORF information:

RefSeq Version	XM_022410888.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022410888.1

ORF Insert Sequence:

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGTCCCAT TTGATGAGAA TGACCTGGAG 
GAAGACGTGG ACTCGGAGCC AGCAAAGATA GAAGGGGAGG CCACAGAGAA TGGGGACACA 
GGGGACACTG GTGCTGAGCT GGACGATGAT CAGCGCTGGT CTGACGGGAT CCCATCGGAT 
GCCGAGACGG ATCTCCGCGT GCAGCACCAG GAGGCGGTAG AGGCAGACCT GGAGCTGAGG 
GTCTCAGAGA ATGAGGAGGA GCCACCTGAG GTCACACCAG GGCATCCGGA GAGAAGTCTC 
ACCCGGGTCC CCAGCCCTGC CCTGTCCCCT GAGGAGCAAG CTGGTCTGCG GTCCCCAGGC 
CACAGCCCTA AGGCAGAGGG GGCCCAAATC CCACTTGTCT CTGTTGTCAC CAAGGCAAAA 
TTGCCTGAAG AGAACCTTTT CCCCAAGCCT TTGCTCCCCA AAGTGAAGCC CAAGGTGGAA 
GTGCCCTGCA GTCAGAGAGC TGCATGCTCC CCCATCCGTT CTCAGCCAGT GGCTCTGCCA 
GAAGCCAGGA CACCTACCTC CCCAGTGAGC TCGCAGTGCC TGTCGCCTTT GGCCACCTCC 
ACCCCCACCA GCACCCAGCT CCCCATTTGC TCCCAGCCTC AGCCTTCCTC TGAGACCACG 
GCCCCATCCC CCACCAAGTC CCCCATACGG CTCCAGCCTG TTTCAGCCAA AACATCTATC 
CCAGTGGTTC CCCTCCCCTT GAAGAACCAA GGGGACACCA AGGACAGGCT GGGCAGCCCT 
CTCGCTGTGG ATGAGGCCCT GAAGCGGAAT GACCTGGTGG CAGAGTTCTG GATGAAGAGC 
GCCGAGATCC GCCGCAGCCT CGGGCTCACA CCAGTGGATC GGAGCAAGGT GTATGAGCCC 
AGCTTCCCTT CGCCTGCCTT TAAACCAGTG TCCCTAAAAT CCTATGCAGT GGAGAAGTCT 
CCCGAGGGGG AGGGACTCCA GCTTCTGAAG CCTCCACCTG TTCCTAAGAG ACTAGGCCTG 
CCGAAGTCAG ACGGTGACCA GCCCTTCCTG CCAACCCCCA AGTCCCCGTC GGACAGAGAG 
CTGAAAAGCT CCCAGGAGGA GAGGAGAGAT CTGTCGAGCA GCTCCGGGCT GGGCCTTCAC 
GGGAGCTCCT CCAACATGAA GACTTTGGGC AGCCAGAGCT TCAACACATC AGACTCCACC 
ATGCTCACTC CCCCTTCAAG CCCACCGCCA CCACCACCCC AAGACGAGGA ACCCGCAACT 
CTTCGGAGGA AGCCATATCA GACTCATGAG CATAAGGACG CTGAGCCCAA GGCCTCGGTG 
ATTCCACCAC CACCACCTGC GACGTTCATG CGGGCCCCCC GGGAGCCCAC CCAGCCCCTC 
CAAGAGGAGA TGCGGAAGTC GTTTGTGGAG AGCGTGGATG AGATCCCATT TGCCGATGAT 
GTGGAGGACA CCTATGATGA CAAGACCGAG GACTCGAGTC TCCAGGAGAA GTTCTTCACA 
CCCCCCTCCT ACTGGCCCCG CACGGAGAGG CCCCTCCACC CACCCCTAGC CAAGGAGAAT 
GGTAGGTTGC CTACTCTGGA GAGTGGGGTC CAGCCACAGA AGAGGGGCCT GCCCTTAGTC 
TCTCCAGAAG CCAAGGAGCT GGCTGAGGAG CGCATGCGAG CCAGGGAGAA GTCTGTGAAG 
AGCCAGGCGT TGAGAGACGC TATGGCCAAG CAGCTGAGTA AGATGAAGGA AATGGAGATG 
GCGGCTGGGA CCTCCAGTCC CCCAGGAGGC ACCTCCCACA AAGCCTCCTC TGTTCCCTCC 
AAGGGCAAAG ACCTCAGCCC CGAGTCCCCT AGACGCCCAG TTCTCAAAGG CCCCAGGGAG 
CCTGCCCTGA AGCACGAAGC CACTAGTGAG GAGGTCCTCT CCCCACCATC GGACTCAGGG 
GGCCCAGATG GTTCGGTCAC CTCATCCGAG GGCTCCAGTG GGAAGAGCAA GAAGAGATCG 
TCACTCTTCT CCCCCCGCAG AAACAAGAAA GAGAAGAAGT CCAAAGGTGA GGGCCGGCCC 
CCGGAGAAGC CCAGCCCAAG CCTTCTGGAG GAAGCTGCTG CCAAGCCCAA GTCCCTGTGG 
AAGTCGGTCT TTTCCGGGTA CAAGAAGGAC AAAAAGAAGA AGGGTGATGA CAAGTCCTGT 
TCCAGCACCC CTTCCAGCGG TGCCACTGTG GACTCTGGCA AGCACAAGAT GTCCCCTGTT 
GTGAGAGCAG AGCTGCACCT GCGGCGCCAG TTGAGCTTCT CCGAGGACTC AGACCTGTCC 
AGTGATGACA TCCTTGAGAG GTCCTCCCAG AAGTCCAGGC GAGAGTCGGT TTATGTACCA 
CACGCCTTGG CATTCAAGAG ATCATATCCA TCAAAGCCGA GAACCTACAC AGAGGAGGAA 
CTGAATGCCA AGCTGACCCG GCGTGTGCAG AAGGCAGCTC GTAGACAGGC CAAGCAAGAG 
GAGCTGAAGC GGCTGCACCG AGCACAGATC ATCCAGAGGC AGCTGGAGCA GGTGGAGGAG 
AAGCAGAGAC AGCTGGAGGA AAGAGGGGTG GCCGTGGAGA AGGCGCTCCG TGGTGAAGCA 
GACTATTGGG GAGAGTCTTA TTACAGTGAC CTCATCGACT TGCATCTGGG TGTTGAGCCC 
AGTGGCGGGA CTCCACGGCG TAGACCTCTC TCCTTCTGCC CTTGCTGTGT CCAGGAAGGC 
ATGGGCAAGA AGGATGACCC CAAGCTGATG CAGGAGTGGT TCAAGTTGGT GCAAGAGAAG 
AATGCCATGG TGCGCTATGA GTCGGAGCTG ATGATTTTTG CCCGGGAGCT GGAACTGGAG 
GACCGGCAGA GCCGACTGCA GCAGGAGCTC CGGGAGCGGA TGGCGGTGGA AGATCACCTG 
AAGACTGAGG AGGAGCTGTC TGAGGAGAAG AAGATCTTGA ACGAGATGCT GGAGGTGGTG 
GAGCAGAGAG ATGCCCTCGT GGCCCTGCTT GAGGAGCAGC GACTCCGGGA GAAGGAGGAG 
GACAAAGACC TGGAGGCTGT CATGCTGTCC AAGGGCTTCA GCCTGAACTG GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_022410889.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022266597.1 Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X20, mRNA.

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CloneID	OCe282685
Clone ID Related Accession (Same CDS sequence)	XM_022410889.1
Accession Version	XM_022410889.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	F-actin-methionine sulfoxide oxidase MICAL3 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGACGTA CAGGGATCTT 
GATGCTGACA GCCGTGGCAA GCAGAGTCCC CACCATGAGA GGGGCTCGAT AAAGAAGGAG 
TTCCCACAGA ACTTAGGAGG CAGCGACACG TGCTATTTCT GCCGGAAGCG GGTCTACGTG 
ATGGAAAGGC TGAGTGCTGA GGGCAAGTTC TTCCATCGGA GCTGCTTCAA GTGCGAGTAC 
TGCGCCACCA CCCTGCGCCT CTCGGCCTAT GCCTATGACA TTGAGGACGG TAAATTCTAC 
TGCAAACCAC ACTACTGTTA TCGACTCTCT GGCTCTGCAC AGAGGAAGAG ACCAGCAGTG 
GCTCCTCTCT CTGGAAAGGA GGCCAGAGAA CCCCTGCAGG ATGGCCCCAC TGTGGACACA 
AATGGACGGG CCAGCGCTGT CACCAGCCCT GCTGAGAGAA CTCCAGGTTC AAGCGTGAAT 
GGTGTGGAAG AGCCTAGCAT TGCCAAGAGG CTCAGGGGCA CTCCCGAGCG GATTGAGCTG 
GAGAACTACC GCCTGTCCGT GAAGCAGGCC GAGGAGCTGG AGGAGGTGCC CGAGGAGACA 
CAGGCCGAGC ACAACCTGAG CAGCGTGCTG GACACGGGGG CAGAGGAGGA CGTGGCCAGC 
AGCAGTTCTG AGTCAGAGAT GGAGGAAGAG GAGGAAGAAG AGCCTCCTCT GCCCACTTCG 
GACCTGGGCG GTGTTCCTTG GAAGGAGGCC GTGAGGATCC ATGCTCTTCT GAAAGGAAAG 
AGTGAAGAGG AGCTAGAGGC CTCAAAGAGC TTTGGGCCTG GGCATGAAGA AGACGAGGAG 
GAGGAGGAGT ATGAAGAGGA GGAGGAGGAG GAGGAGGAAG AATATGAAGA GGAAGAGGAA 
GAGGAAGAGT CTAGTGAAGA AGGGGAGTAT TGCCCCTGGG ACAGAGAACT CCTACAAGGC 
CAGTGGCTCC GGCAGCTGTC ACAGGAAGAA ACTACGGGTA CACGTAAAGT TGGGAGCCAG 
AGGCTACAGC AGATCGTAAA TCCGGCCGAC CCCTTGGAGA TCCAGGCTGA TGTGCACTGG 
ACACATATCC GAGAGAGAGA GGAGGAGGAG CGAATGGTCC CAACCTCTGA GTCCTCCACT 
TCTAGAGTGC CTGACCTTCC CTCCATACGC CGCGCAGCAG TGCAGGCCTG GCTGGAGACG 
GTCTCTGGAG TCCCATTTGA TGAGAATGAC CTGGAGGAAG ACGTGGACTC GGAGCCAGCA 
AAGATAGAAG GGGAGGCCAC AGAGAATGGG GACACAGGGG ACACTGGTGC TGAGCTGGAC 
GATGATCAGC GCTGGTCTGA CGGGATCCCA TCGGATGCCG AGACGGATCT CCGCGTGCAG 
CACCAGGAGG CGGTAGAGGC AGACCTGGAG CTGAGGGTCT CAGAGAATGA GGAGGAGCCA 
CCTGAGGTCA CACCAGGGCA TCCGGAGAGA AGTCTCACCC GGGTCCCCAG CCCTGCCCTG 
TCCCCTGAGG AGCAAGCTGG TCTGCGGTCC CCAGGCCACA GCCCTAAGGC AGAGGGGGCC 
CAAATCCCAC TTGTCTCTGT TGTCACCAAG GCAAAATTGC CTGAAGAGAA CCTTTTCCCC 
AAGCCTTTGC TCCCCAAAGT GAAGCCCAAG GTGGAAGTGC CCTGCAGTCA GAGAGCTGCA 
TGCTCCCCCA TCCGTTCTCA GCCAGTGGCT CTGCCAGAAG CCAGGACACC TACCTCCCCA 
GTGAGCTCGC AGTGCCTGTC GCCTTTGGCC ACCTCCACCC CCACCAGCAC CCAGCTCCCC 
ATTTGCTCCC AGCCTCAGCC TTCCTCTGAG ACCACGGCCC CATCCCCCAC CAAGTCCCCC 
ATACGGCTCC AGCCTGTTTC AGCCAAAACA TCTATCCCAG TGGTTCCCCT CCCCTTGAAG 
AACCAAGGGG ACACCAAGGA CAGGCTGGGC AGCCCTCTCG CTGTGGATGA GGCCCTGAAG 
CGGAATGACC TGGTGGCAGA GTTCTGGATG AAGAGCGCCG AGATCCGCCG CAGCCTCGGG 
CTCACACCAG TGGATCGGAG CAAGGTGTAT GAGCCCAGCT TCCCTTCGCC TGCCTTTAAA 
CCAGTGTCCC TAAAATCCTA TGCAGTGGAG AAGTCTCCCG AGGGGGAGGG ACTCCAGCTT 
CTGAAGCCTC CACCTGTTCC TAAGAGACTA GGCCTGCCGA AGTCAGACGG TGACCAGCCC 
TTCCTGCCAA CCCCCAAGTC CCCGTCGGAC AGAGAGCTGA AAAGCTCCCA GGAGGAGAGG 
AGAGATCTGT CGAGCAGCTC CGGGCTGGGC CTTCACGGGA GCTCCTCCAA CATGAAGACT 
TTGGGCAGCC AGAGCTTCAA CACATCAGAC TCCACCATGC TCACTCCCCC TTCAAGCCCA 
CCGCCACCAC CACCCCAAGA CGAGGAACCC GCAACTCTTC GGAGGAAGCC ATATCAGACT 
CATGAGCATA AGGACGCTGA GCCCAAGGCC TCGGTGATTC CACCACCACC ACCTGCGACG 
TTCATGCGGG CCCCCCGGGA GCCCACCCAG CCCCTCCAAG AGGAGATGCG GAAGTCGTTT 
GTGGAGAGCG TGGATGAGAT CCCATTTGCC GATGATGTGG AGGACACCTA TGATGACAAG 
ACCGAGGACT CGAGTCTCCA GGAGAAGTTC TTCACACCCC CCTCCTACTG GCCCCGCACG 
GAGAGGCCCC TCCACCCACC CCTAGCCAAG GAGAATGGTA GGTTGCCTAC TCTGGAGAGT 
GGGGTCCAGC CACAGAAGAG GGGCCTGCCC TTAGTCTCTC CAGAAGCCAA GGAGCTGGCT 
GAGGAGCGCA TGCGAGCCAG GGAGAAGTCT GTGAAGAGCC AGGCGTTGAG AGACGCTATG 
GCCAAGCAGC TGAGTAAGAT GAAGGAAATG GAGATGGCGG CTGGGACCTC CAGTCCCCCA 
GGAGGCACCT CCCACAAAGC CTCCTCTGTT CCCTCCAAGG GCAAAGACCT CAGCCCCGAG 
TCCCCTAGAC GCCCAGTTCT CAAAGGCCCC AGGGAGCCTG CCCTGAAGCA CGAAGCCACT 
AGTGAGGAGG TCCTCTCCCC ACCATCGGAC TCAGGGGGCC CAGATGGTTC GGTCACCTCA 
TCCGAGGGCT CCAGTGGGAA GAGCAAGAAG AGATCGTCAC TCTTCTCCCC CCGCAGAAAC 
AAGAAAGAGA AGAAGTCCAA AGGTGAGGGC CGGCCCCCGG AGAAGCCCAG CCCAAGCCTT 
CTGGAGGAAG CTGCTGCCAA GCCCAAGTCC CTGTGGAAGT CGGTCTTTTC CGGGTACAAG 
AAGGACAAAA AGAAGAAGGG TGATGACAAG TCCTGTTCCA GCACCCCTTC CAGCGGTGCC 
ACTGTGGACT CTGGCAAGCA CAAGATGTCC CCTGTTGTGA GAGCAGAGCT GCACCTGCGG 
CGCCAGTTGA GCTTCTCCGA GGACTCAGAC CTGTCCAGTG ATGACATCCT TGAGAGGTCC 
TCCCAGAAGT CCAGGCGAGA GTCGGTTTAT GTACCACACG CCTTGGCATT CAAGAGATCA 
TATCCATCAA AGCCGAGAAC CTACACAGAG GAGGAACTGA ATGCCAAGCT GACCCGGCGT 
GTGCAGAAGG CAGCTCGTAG ACAGGCCAAG CAAGAGGAGC TGAAGCGGCT GCACCGAGCA 
CAGATCATCC AGAGGCAGCT GGAGCAGGTG GAGGAGAAGC AGAGACAGCT GGAGGAAAGA 
GGGGTGGCCG TGGAGAAGGC GCTCCGTGGT GAAGCAGACT ATTGGGGAGA GTCTTATTAC 
AGTGACCTCA TCGACTTGCA TCTGGGTGTT GAGCCCAGTG GCGGGACTCC ACGGCGTAGA 
CCTCTCTCCT TCTGCCCTTG CTGTGTCCAG GAAGGCATGG GCAAGAAGGA TGACCCCAAG 
CTGATGCAGG AGTGGTTCAA GTTGGTGCAA GAGAAGAATG CCATGGTGCG CTATGAGTCG 
GAGCTGATGA TTTTTGCCCG GGAGCTGGAA CTGGAGGACC GGCAGAGCCG ACTGCAGCAG 
GAGCTCCGGG AGCGGATGGC GGTGGAAGAT CACCTGAAGA CTGAGGAGGA GCTGTCTGAG 
GAGAAGAAGA TCTTGAACGA GATGCTGGAG GTGGTGGAGC AGAGAGATGC CCTCGTGGCC 
CTGCTTGAGG AGCAGCGACT CCGGGAGAAG GAGGAGGACA AAGACCTGGA GGCTGTCATG 
CTGTCCAAGG GCTTCAGCCT GAACTGGTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022266597.1
CDS	258..6770
Translation	MEDSKNETVNHAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDYRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKACTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFQGLVQPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELKEATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLNYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREQNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNININFLRPSQVRHLYNTGETRDIHLEMENLVNSRTTPKLTRNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGKEMASVGEPDKLSMVMYLTQFYEMFKDSLPSSDALDLNAEEKAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEEAPRGYRGGRPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSTGMRRQPTQERGVSQPSCCLPEQGHPAPTPQWKQTYRDLDADSRGKQSPHHERGSIKKEFPQNLGGSDTCYFCRKRVYVMERLSAEGKFFHRSCFKCEYCATTLRLSAYAYDIEDGKFYCKPHYCYRLSGSAQRKRPAVAPLSGKEAREPLQDGPTVDTNGRASAVTSPAERTPGSSVNGVEEPSIAKRLRGTPERIELENYRLSVKQAEELEEVPEETQAEHNLSSVLDTGAEEDVASSSSESEMEEEEEEEPPLPTSDLGGVPWKEAVRIHALLKGKSEEELEASKSFGPGHEEDEEEEEYEEEEEEEEEEYEEEEEEEESSEEGEYCPWDRELLQGQWLRQLSQEETTGTRKVGSQRLQQIVNPADPLEIQADVHWTHIREREEEERMVPTSESSTSRVPDLPSIRRAAVQAWLETVSGVPFDENDLEEDVDSEPAKIEGEATENGDTGDTGAELDDDQRWSDGIPSDAETDLRVQHQEAVEADLELRVSENEEEPPEVTPGHPERSLTRVPSPALSPEEQAGLRSPGHSPKAEGAQIPLVSVVTKAKLPEENLFPKPLLPKVKPKVEVPCSQRAACSPIRSQPVALPEARTPTSPVSSQCLSPLATSTPTSTQLPICSQPQPSSETTAPSPTKSPIRLQPVSAKTSIPVVPLPLKNQGDTKDRLGSPLAVDEALKRNDLVAEFWMKSAEIRRSLGLTPVDRSKVYEPSFPSPAFKPVSLKSYAVEKSPEGEGLQLLKPPPVPKRLGLPKSDGDQPFLPTPKSPSDRELKSSQEERRDLSSSSGLGLHGSSSNMKTLGSQSFNTSDSTMLTPPSSPPPPPPQDEEPATLRRKPYQTHEHKDAEPKASVIPPPPPATFMRAPREPTQPLQEEMRKSFVESVDEIPFADDVEDTYDDKTEDSSLQEKFFTPPSYWPRTERPLHPPLAKENGRLPTLESGVQPQKRGLPLVSPEAKELAEERMRAREKSVKSQALRDAMAKQLSKMKEMEMAAGTSSPPGGTSHKASSVPSKGKDLSPESPRRPVLKGPREPALKHEATSEEVLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRNKKEKKSKGEGRPPEKPSPSLLEEAAAKPKSLWKSVFSGYKKDKKKKGDDKSCSSTPSSGATVDSGKHKMSPVVRAELHLRRQLSFSEDSDLSSDDILERSSQKSRRESVYVPHALAFKRSYPSKPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELSEEKKILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAVMLSKGFSLNWS

Target ORF information:

RefSeq Version	XM_022410889.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022410889.1

ORF Insert Sequence:

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGACGTA CAGGGATCTT 
GATGCTGACA GCCGTGGCAA GCAGAGTCCC CACCATGAGA GGGGCTCGAT AAAGAAGGAG 
TTCCCACAGA ACTTAGGAGG CAGCGACACG TGCTATTTCT GCCGGAAGCG GGTCTACGTG 
ATGGAAAGGC TGAGTGCTGA GGGCAAGTTC TTCCATCGGA GCTGCTTCAA GTGCGAGTAC 
TGCGCCACCA CCCTGCGCCT CTCGGCCTAT GCCTATGACA TTGAGGACGG TAAATTCTAC 
TGCAAACCAC ACTACTGTTA TCGACTCTCT GGCTCTGCAC AGAGGAAGAG ACCAGCAGTG 
GCTCCTCTCT CTGGAAAGGA GGCCAGAGAA CCCCTGCAGG ATGGCCCCAC TGTGGACACA 
AATGGACGGG CCAGCGCTGT CACCAGCCCT GCTGAGAGAA CTCCAGGTTC AAGCGTGAAT 
GGTGTGGAAG AGCCTAGCAT TGCCAAGAGG CTCAGGGGCA CTCCCGAGCG GATTGAGCTG 
GAGAACTACC GCCTGTCCGT GAAGCAGGCC GAGGAGCTGG AGGAGGTGCC CGAGGAGACA 
CAGGCCGAGC ACAACCTGAG CAGCGTGCTG GACACGGGGG CAGAGGAGGA CGTGGCCAGC 
AGCAGTTCTG AGTCAGAGAT GGAGGAAGAG GAGGAAGAAG AGCCTCCTCT GCCCACTTCG 
GACCTGGGCG GTGTTCCTTG GAAGGAGGCC GTGAGGATCC ATGCTCTTCT GAAAGGAAAG 
AGTGAAGAGG AGCTAGAGGC CTCAAAGAGC TTTGGGCCTG GGCATGAAGA AGACGAGGAG 
GAGGAGGAGT ATGAAGAGGA GGAGGAGGAG GAGGAGGAAG AATATGAAGA GGAAGAGGAA 
GAGGAAGAGT CTAGTGAAGA AGGGGAGTAT TGCCCCTGGG ACAGAGAACT CCTACAAGGC 
CAGTGGCTCC GGCAGCTGTC ACAGGAAGAA ACTACGGGTA CACGTAAAGT TGGGAGCCAG 
AGGCTACAGC AGATCGTAAA TCCGGCCGAC CCCTTGGAGA TCCAGGCTGA TGTGCACTGG 
ACACATATCC GAGAGAGAGA GGAGGAGGAG CGAATGGTCC CAACCTCTGA GTCCTCCACT 
TCTAGAGTGC CTGACCTTCC CTCCATACGC CGCGCAGCAG TGCAGGCCTG GCTGGAGACG 
GTCTCTGGAG TCCCATTTGA TGAGAATGAC CTGGAGGAAG ACGTGGACTC GGAGCCAGCA 
AAGATAGAAG GGGAGGCCAC AGAGAATGGG GACACAGGGG ACACTGGTGC TGAGCTGGAC 
GATGATCAGC GCTGGTCTGA CGGGATCCCA TCGGATGCCG AGACGGATCT CCGCGTGCAG 
CACCAGGAGG CGGTAGAGGC AGACCTGGAG CTGAGGGTCT CAGAGAATGA GGAGGAGCCA 
CCTGAGGTCA CACCAGGGCA TCCGGAGAGA AGTCTCACCC GGGTCCCCAG CCCTGCCCTG 
TCCCCTGAGG AGCAAGCTGG TCTGCGGTCC CCAGGCCACA GCCCTAAGGC AGAGGGGGCC 
CAAATCCCAC TTGTCTCTGT TGTCACCAAG GCAAAATTGC CTGAAGAGAA CCTTTTCCCC 
AAGCCTTTGC TCCCCAAAGT GAAGCCCAAG GTGGAAGTGC CCTGCAGTCA GAGAGCTGCA 
TGCTCCCCCA TCCGTTCTCA GCCAGTGGCT CTGCCAGAAG CCAGGACACC TACCTCCCCA 
GTGAGCTCGC AGTGCCTGTC GCCTTTGGCC ACCTCCACCC CCACCAGCAC CCAGCTCCCC 
ATTTGCTCCC AGCCTCAGCC TTCCTCTGAG ACCACGGCCC CATCCCCCAC CAAGTCCCCC 
ATACGGCTCC AGCCTGTTTC AGCCAAAACA TCTATCCCAG TGGTTCCCCT CCCCTTGAAG 
AACCAAGGGG ACACCAAGGA CAGGCTGGGC AGCCCTCTCG CTGTGGATGA GGCCCTGAAG 
CGGAATGACC TGGTGGCAGA GTTCTGGATG AAGAGCGCCG AGATCCGCCG CAGCCTCGGG 
CTCACACCAG TGGATCGGAG CAAGGTGTAT GAGCCCAGCT TCCCTTCGCC TGCCTTTAAA 
CCAGTGTCCC TAAAATCCTA TGCAGTGGAG AAGTCTCCCG AGGGGGAGGG ACTCCAGCTT 
CTGAAGCCTC CACCTGTTCC TAAGAGACTA GGCCTGCCGA AGTCAGACGG TGACCAGCCC 
TTCCTGCCAA CCCCCAAGTC CCCGTCGGAC AGAGAGCTGA AAAGCTCCCA GGAGGAGAGG 
AGAGATCTGT CGAGCAGCTC CGGGCTGGGC CTTCACGGGA GCTCCTCCAA CATGAAGACT 
TTGGGCAGCC AGAGCTTCAA CACATCAGAC TCCACCATGC TCACTCCCCC TTCAAGCCCA 
CCGCCACCAC CACCCCAAGA CGAGGAACCC GCAACTCTTC GGAGGAAGCC ATATCAGACT 
CATGAGCATA AGGACGCTGA GCCCAAGGCC TCGGTGATTC CACCACCACC ACCTGCGACG 
TTCATGCGGG CCCCCCGGGA GCCCACCCAG CCCCTCCAAG AGGAGATGCG GAAGTCGTTT 
GTGGAGAGCG TGGATGAGAT CCCATTTGCC GATGATGTGG AGGACACCTA TGATGACAAG 
ACCGAGGACT CGAGTCTCCA GGAGAAGTTC TTCACACCCC CCTCCTACTG GCCCCGCACG 
GAGAGGCCCC TCCACCCACC CCTAGCCAAG GAGAATGGTA GGTTGCCTAC TCTGGAGAGT 
GGGGTCCAGC CACAGAAGAG GGGCCTGCCC TTAGTCTCTC CAGAAGCCAA GGAGCTGGCT 
GAGGAGCGCA TGCGAGCCAG GGAGAAGTCT GTGAAGAGCC AGGCGTTGAG AGACGCTATG 
GCCAAGCAGC TGAGTAAGAT GAAGGAAATG GAGATGGCGG CTGGGACCTC CAGTCCCCCA 
GGAGGCACCT CCCACAAAGC CTCCTCTGTT CCCTCCAAGG GCAAAGACCT CAGCCCCGAG 
TCCCCTAGAC GCCCAGTTCT CAAAGGCCCC AGGGAGCCTG CCCTGAAGCA CGAAGCCACT 
AGTGAGGAGG TCCTCTCCCC ACCATCGGAC TCAGGGGGCC CAGATGGTTC GGTCACCTCA 
TCCGAGGGCT CCAGTGGGAA GAGCAAGAAG AGATCGTCAC TCTTCTCCCC CCGCAGAAAC 
AAGAAAGAGA AGAAGTCCAA AGGTGAGGGC CGGCCCCCGG AGAAGCCCAG CCCAAGCCTT 
CTGGAGGAAG CTGCTGCCAA GCCCAAGTCC CTGTGGAAGT CGGTCTTTTC CGGGTACAAG 
AAGGACAAAA AGAAGAAGGG TGATGACAAG TCCTGTTCCA GCACCCCTTC CAGCGGTGCC 
ACTGTGGACT CTGGCAAGCA CAAGATGTCC CCTGTTGTGA GAGCAGAGCT GCACCTGCGG 
CGCCAGTTGA GCTTCTCCGA GGACTCAGAC CTGTCCAGTG ATGACATCCT TGAGAGGTCC 
TCCCAGAAGT CCAGGCGAGA GTCGGTTTAT GTACCACACG CCTTGGCATT CAAGAGATCA 
TATCCATCAA AGCCGAGAAC CTACACAGAG GAGGAACTGA ATGCCAAGCT GACCCGGCGT 
GTGCAGAAGG CAGCTCGTAG ACAGGCCAAG CAAGAGGAGC TGAAGCGGCT GCACCGAGCA 
CAGATCATCC AGAGGCAGCT GGAGCAGGTG GAGGAGAAGC AGAGACAGCT GGAGGAAAGA 
GGGGTGGCCG TGGAGAAGGC GCTCCGTGGT GAAGCAGACT ATTGGGGAGA GTCTTATTAC 
AGTGACCTCA TCGACTTGCA TCTGGGTGTT GAGCCCAGTG GCGGGACTCC ACGGCGTAGA 
CCTCTCTCCT TCTGCCCTTG CTGTGTCCAG GAAGGCATGG GCAAGAAGGA TGACCCCAAG 
CTGATGCAGG AGTGGTTCAA GTTGGTGCAA GAGAAGAATG CCATGGTGCG CTATGAGTCG 
GAGCTGATGA TTTTTGCCCG GGAGCTGGAA CTGGAGGACC GGCAGAGCCG ACTGCAGCAG 
GAGCTCCGGG AGCGGATGGC GGTGGAAGAT CACCTGAAGA CTGAGGAGGA GCTGTCTGAG 
GAGAAGAAGA TCTTGAACGA GATGCTGGAG GTGGTGGAGC AGAGAGATGC CCTCGTGGCC 
CTGCTTGAGG AGCAGCGACT CCGGGAGAAG GAGGAGGACA AAGACCTGGA GGCTGTCATG 
CTGTCCAAGG GCTTCAGCCT GAACTGGTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_022410890.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022266598.1 Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X21, mRNA.

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CloneID	OCe282686
Clone ID Related Accession (Same CDS sequence)	XM_022410890.1
Accession Version	XM_022410890.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	F-actin-methionine sulfoxide oxidase MICAL3 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GAAGTATATA AAGATGTACA CGGGCGGAGT GAGCTCATTG 
GCTGAGCAGA TAGCCAATCA GCTTCAAAGG AAAGAACAGC CCAAAACCCT TCTAGACAAG 
AAGGAACTGG GCTCGATAAA GAAGGAGTTC CCACAGAACT TAGGAGGCAG CGACACGTGC 
TATTTCTGCC GGAAGCGGGT CTACGTGATG GAAAGGCTGA GTGCTGAGGG CAAGTTCTTC 
CATCGGAGCT GCTTCAAGTG CGAGTACTGC GCCACCACCC TGCGCCTCTC GGCCTATGCC 
TATGACATTG AGGACGGTAA ATTCTACTGC AAACCACACT ACTGTTATCG ACTCTCTGGC 
TCTGCACAGA GGAAGAGACC AGCAGTGGCT CCTCTCTCTG GAAAGGAGGC CAGAGAACCC 
CTGCAGGATG GCCCCACTGT GGACACAAAT GGACGGGCCA GCGCTGTCAC CAGCCCTGCT 
GAGAGAACTC CAGGTTCAAG CGTGAATGGT GTGGAAGAGC CTAGCATTGC CAAGAGGCTC 
AGGGGCACTC CCGAGCGGAT TGAGCTGGAG AACTACCGCC TGTCCGTGAA GCAGGCCGAG 
GAGCTGGAGG AGGTGCCCGA GGAGACACAG GCCGAGCACA ACCTGAGCAG CGTGCTGGAC 
ACGGGGGCAG AGGAGGACGT GGCCAGCAGC AGTTCTGAGT CAGAGATGGA GGAAGAGGAG 
GAAGAAGAGC CTCCTCTGCC CACTTCGGAC CTGGGCGGTG TTCCTTGGAA GGAGGCCGTG 
AGGATCCATG CTCTTCTGAA AGGAAAGAGT GAAGAGGAGC TAGAGGCCTC AAAGAGCTTT 
GGGCCTGGGC ATGAAGAAGA CGAGGAGGAG GAGGAGTATG AAGAGGAGGA GGAGGAGGAG 
GAGGAAGAAT ATGAAGAGGA AGAGGAAGAG GAAGAGTCTA GTGAAGAAGG GGAGTATTGC 
CCCTGGGACA GAGAACTCCT ACAAGGCCAG TGGCTCCGGC AGCTGTCACA GGAAGAAACT 
ACGGGTACAC GTAAAGTTGG GAGCCAGAGG CTACAGCAGA TCGTAAATCC GGCCGACCCC 
TTGGAGATCC AGGCTGATGT GCACTGGACA CATATCCGAG AGAGAGAGGA GGAGGAGCGA 
ATGGTCCCAA CCTCTGAGTC CTCCACTTCT AGAGTGCCTG ACCTTCCCTC CATACGCCGC 
GCAGCAGTGC AGGCCTGGCT GGAGACGGTC TCTGGAGTCC CATTTGATGA GAATGACCTG 
GAGGAAGACG TGGACTCGGA GCCAGCAAAG ATAGAAGGGG AGGCCACAGA GAATGGGGAC 
ACAGGGGACA CTGGTGCTGA GCTGGACGAT GATCAGCGCT GGTCTGACGG GATCCCATCG 
GATGCCGAGA CGGATCTCCG CGTGCAGCAC CAGGAGGCGG TAGAGGCAGA CCTGGAGCTG 
AGGGTCTCAG AGAATGAGGA GGAGCCACCT GAGGTCACAC CAGGGCATCC GGAGAGAAGT 
CTCACCCGGG TCCCCAGCCC TGCCCTGTCC CCTGAGGAGC AAGCTGGTCT GCGGTCCCCA 
GGCCACAGCC CTAAGGCAGA GGGGGCCCAA ATCCCACTTG TCTCTGTTGT CACCAAGGCA 
AAATTGCCTG AAGAGAACCT TTTCCCCAAG CCTTTGCTCC CCAAAGTGAA GCCCAAGGTG 
GAAGTGCCCT GCAGTCAGAG AGCTGCATGC TCCCCCATCC GTTCTCAGCC AGTGGCTCTG 
CCAGAAGCCA GGACACCTAC CTCCCCAGTG AGCTCGCAGT GCCTGTCGCC TTTGGCCACC 
TCCACCCCCA CCAGCACCCA GCTCCCCATT TGCTCCCAGC CTCAGCCTTC CTCTGAGACC 
ACGGCCCCAT CCCCCACCAA GTCCCCCATA CGGCTCCAGC CTGTTTCAGC CAAAACATCT 
ATCCCAGTGG TTCCCCTCCC CTTGAAGAAC CAAGGGGACA CCAAGGACAG GCTGGGCAGC 
CCTCTCGCTG TGGATGAGGC CCTGAAGCGG AATGACCTGG TGGCAGAGTT CTGGATGAAG 
AGCGCCGAGA TCCGCCGCAG CCTCGGGCTC ACACCAGTGG ATCGGAGCAA GGTGTATGAG 
CCCAGCTTCC CTTCGCCTGC CTTTAAACCA GTGTCCCTAA AATCCTATGC AGTGGAGAAG 
TCTCCCGAGG GGGAGGGACT CCAGCTTCTG AAGCCTCCAC CTGTTCCTAA GAGACTAGGC 
CTGCCGAAGT CAGACGGTGA CCAGCCCTTC CTGCCAACCC CCAAGTCCCC GTCGGACAGA 
GAGCTGAAAA GCTCCCAGGA GGAGAGGAGA GATCTGTCGA GCAGCTCCGG GCTGGGCCTT 
CACGGGAGCT CCTCCAACAT GAAGACTTTG GGCAGCCAGA GCTTCAACAC ATCAGACTCC 
ACCATGCTCA CTCCCCCTTC AAGCCCACCG CCACCACCAC CCCAAGACGA GGAACCCGCA 
ACTCTTCGGA GGAAGCCATA TCAGACTCAT GAGCATAAGG ACGCTGAGCC CAAGGCCTCG 
GTGATTCCAC CACCACCACC TGCGACGTTC ATGCGGGCCC CCCGGGAGCC CACCCAGCCC 
CTCCAAGAGG AGATGCGGAA GTCGTTTGTG GAGAGCGTGG ATGAGATCCC ATTTGCCGAT 
GATGTGGAGG ACACCTATGA TGACAAGACC GAGGACTCGA GTCTCCAGGA GAAGTTCTTC 
ACACCCCCCT CCTACTGGCC CCGCACGGAG AGGCCCCTCC ACCCACCCCT AGCCAAGGAG 
AATGGTAGGT TGCCTACTCT GGAGAGTGGG GTCCAGCCAC AGAAGAGGGG CCTGCCCTTA 
GTCTCTCCAG AAGCCAAGGA GCTGGCTGAG GAGCGCATGC GAGCCAGGGA GAAGTCTGTG 
AAGAGCCAGG CGTTGAGAGA CGCTATGGCC AAGCAGCTGA GTAAGATGAA GGAAATGGAG 
ATGGCGGCTG GGACCTCCAG TCCCCCAGGA GGCACCTCCC ACAAAGCCTC CTCTGTTCCC 
TCCAAGGGCA AAGACCTCAG CCCCGAGTCC CCTAGACGCC CAGTTCTCAA AGGCCCCAGG 
GAGCCTGCCC TGAAGCACGA AGCCACTAGT GAGGAGGTCC TCTCCCCACC ATCGGACTCA 
GGGGGCCCAG ATGGTTCGGT CACCTCATCC GAGGGCTCCA GTGGGAAGAG CAAGAAGAGA 
TCGTCACTCT TCTCCCCCCG CAGAAACAAG AAAGAGAAGA AGTCCAAAGG TGAGGGCCGG 
CCCCCGGAGA AGCCCAGCCC AAGCCTTCTG GAGGAAGCTG CTGCCAAGCC CAAGTCCCTG 
TGGAAGTCGG TCTTTTCCGG GTACAAGAAG GACAAAAAGA AGAAGGGTGA TGACAAGTCC 
TGTTCCAGCA CCCCTTCCAG CGGTGCCACT GTGGACTCTG GCAAGCACAA GATGTCCCCT 
GTTGTGAGAG CAGAGCTGCA CCTGCGGCGC CAGTTGAGCT TCTCCGAGGA CTCAGACCTG 
TCCAGTGATG ACATCCTTGA GAGGTCCTCC CAGAAGTCCA GGCGAGAGTC GGTTTATGTA 
CCACACGCCT TGGCATTCAA GAGATCATAT CCATCAAAGC CGAGAACCTA CACAGAGGAG 
GAACTGAATG CCAAGCTGAC CCGGCGTGTG CAGAAGGCAG CTCGTAGACA GGCCAAGCAA 
GAGGAGCTGA AGCGGCTGCA CCGAGCACAG ATCATCCAGA GGCAGCTGGA GCAGGTGGAG 
GAGAAGCAGA GACAGCTGGA GGAAAGAGGG GTGGCCGTGG AGAAGGCGCT CCGTGGTGAA 
GCAGACTATT GGGGAGAGTC TTATTACAGT GACCTCATCG ACTTGCATCT GGGTGTTGAG 
CCCAGTGGCG GGACTCCACG GCGTAGACCT CTCTCCTTCT GCCCTTGCTG TGTCCAGGAA 
GGCATGGGCA AGAAGGATGA CCCCAAGCTG ATGCAGGAGT GGTTCAAGTT GGTGCAAGAG 
AAGAATGCCA TGGTGCGCTA TGAGTCGGAG CTGATGATTT TTGCCCGGGA GCTGGAACTG 
GAGGACCGGC AGAGCCGACT GCAGCAGGAG CTCCGGGAGC GGATGGCGGT GGAAGATCAC 
CTGAAGACTG AGGAGGAGCT GTCTGAGGAG AAGAAGATCT TGAACGAGAT GCTGGAGGTG 
GTGGAGCAGA GAGATGCCCT CGTGGCCCTG CTTGAGGAGC AGCGACTCCG GGAGAAGGAG 
GAGGACAAAG ACCTGGAGGC TGTCATGCTG TCCAAGGGCT TCAGCCTGAA CTGGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022266598.1
CDS	258..6737
Translation	MEDSKNETVNHAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDYRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKACTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFQGLVQPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELKEATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLNYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREQNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNININFLRPSQVRHLYNTGETRDIHLEMENLVNSRTTPKLTRNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGKEMASVGEPDKLSMVMYLTQFYEMFKDSLPSSDALDLNAEEKAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEEAPRGYRGGRPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSTGMRRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCRKRVYVMERLSAEGKFFHRSCFKCEYCATTLRLSAYAYDIEDGKFYCKPHYCYRLSGSAQRKRPAVAPLSGKEAREPLQDGPTVDTNGRASAVTSPAERTPGSSVNGVEEPSIAKRLRGTPERIELENYRLSVKQAEELEEVPEETQAEHNLSSVLDTGAEEDVASSSSESEMEEEEEEEPPLPTSDLGGVPWKEAVRIHALLKGKSEEELEASKSFGPGHEEDEEEEEYEEEEEEEEEEYEEEEEEEESSEEGEYCPWDRELLQGQWLRQLSQEETTGTRKVGSQRLQQIVNPADPLEIQADVHWTHIREREEEERMVPTSESSTSRVPDLPSIRRAAVQAWLETVSGVPFDENDLEEDVDSEPAKIEGEATENGDTGDTGAELDDDQRWSDGIPSDAETDLRVQHQEAVEADLELRVSENEEEPPEVTPGHPERSLTRVPSPALSPEEQAGLRSPGHSPKAEGAQIPLVSVVTKAKLPEENLFPKPLLPKVKPKVEVPCSQRAACSPIRSQPVALPEARTPTSPVSSQCLSPLATSTPTSTQLPICSQPQPSSETTAPSPTKSPIRLQPVSAKTSIPVVPLPLKNQGDTKDRLGSPLAVDEALKRNDLVAEFWMKSAEIRRSLGLTPVDRSKVYEPSFPSPAFKPVSLKSYAVEKSPEGEGLQLLKPPPVPKRLGLPKSDGDQPFLPTPKSPSDRELKSSQEERRDLSSSSGLGLHGSSSNMKTLGSQSFNTSDSTMLTPPSSPPPPPPQDEEPATLRRKPYQTHEHKDAEPKASVIPPPPPATFMRAPREPTQPLQEEMRKSFVESVDEIPFADDVEDTYDDKTEDSSLQEKFFTPPSYWPRTERPLHPPLAKENGRLPTLESGVQPQKRGLPLVSPEAKELAEERMRAREKSVKSQALRDAMAKQLSKMKEMEMAAGTSSPPGGTSHKASSVPSKGKDLSPESPRRPVLKGPREPALKHEATSEEVLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRNKKEKKSKGEGRPPEKPSPSLLEEAAAKPKSLWKSVFSGYKKDKKKKGDDKSCSSTPSSGATVDSGKHKMSPVVRAELHLRRQLSFSEDSDLSSDDILERSSQKSRRESVYVPHALAFKRSYPSKPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELSEEKKILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAVMLSKGFSLNWS

Target ORF information:

RefSeq Version	XM_022410890.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022410890.1

ORF Insert Sequence:

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GAAGTATATA AAGATGTACA CGGGCGGAGT GAGCTCATTG 
GCTGAGCAGA TAGCCAATCA GCTTCAAAGG AAAGAACAGC CCAAAACCCT TCTAGACAAG 
AAGGAACTGG GCTCGATAAA GAAGGAGTTC CCACAGAACT TAGGAGGCAG CGACACGTGC 
TATTTCTGCC GGAAGCGGGT CTACGTGATG GAAAGGCTGA GTGCTGAGGG CAAGTTCTTC 
CATCGGAGCT GCTTCAAGTG CGAGTACTGC GCCACCACCC TGCGCCTCTC GGCCTATGCC 
TATGACATTG AGGACGGTAA ATTCTACTGC AAACCACACT ACTGTTATCG ACTCTCTGGC 
TCTGCACAGA GGAAGAGACC AGCAGTGGCT CCTCTCTCTG GAAAGGAGGC CAGAGAACCC 
CTGCAGGATG GCCCCACTGT GGACACAAAT GGACGGGCCA GCGCTGTCAC CAGCCCTGCT 
GAGAGAACTC CAGGTTCAAG CGTGAATGGT GTGGAAGAGC CTAGCATTGC CAAGAGGCTC 
AGGGGCACTC CCGAGCGGAT TGAGCTGGAG AACTACCGCC TGTCCGTGAA GCAGGCCGAG 
GAGCTGGAGG AGGTGCCCGA GGAGACACAG GCCGAGCACA ACCTGAGCAG CGTGCTGGAC 
ACGGGGGCAG AGGAGGACGT GGCCAGCAGC AGTTCTGAGT CAGAGATGGA GGAAGAGGAG 
GAAGAAGAGC CTCCTCTGCC CACTTCGGAC CTGGGCGGTG TTCCTTGGAA GGAGGCCGTG 
AGGATCCATG CTCTTCTGAA AGGAAAGAGT GAAGAGGAGC TAGAGGCCTC AAAGAGCTTT 
GGGCCTGGGC ATGAAGAAGA CGAGGAGGAG GAGGAGTATG AAGAGGAGGA GGAGGAGGAG 
GAGGAAGAAT ATGAAGAGGA AGAGGAAGAG GAAGAGTCTA GTGAAGAAGG GGAGTATTGC 
CCCTGGGACA GAGAACTCCT ACAAGGCCAG TGGCTCCGGC AGCTGTCACA GGAAGAAACT 
ACGGGTACAC GTAAAGTTGG GAGCCAGAGG CTACAGCAGA TCGTAAATCC GGCCGACCCC 
TTGGAGATCC AGGCTGATGT GCACTGGACA CATATCCGAG AGAGAGAGGA GGAGGAGCGA 
ATGGTCCCAA CCTCTGAGTC CTCCACTTCT AGAGTGCCTG ACCTTCCCTC CATACGCCGC 
GCAGCAGTGC AGGCCTGGCT GGAGACGGTC TCTGGAGTCC CATTTGATGA GAATGACCTG 
GAGGAAGACG TGGACTCGGA GCCAGCAAAG ATAGAAGGGG AGGCCACAGA GAATGGGGAC 
ACAGGGGACA CTGGTGCTGA GCTGGACGAT GATCAGCGCT GGTCTGACGG GATCCCATCG 
GATGCCGAGA CGGATCTCCG CGTGCAGCAC CAGGAGGCGG TAGAGGCAGA CCTGGAGCTG 
AGGGTCTCAG AGAATGAGGA GGAGCCACCT GAGGTCACAC CAGGGCATCC GGAGAGAAGT 
CTCACCCGGG TCCCCAGCCC TGCCCTGTCC CCTGAGGAGC AAGCTGGTCT GCGGTCCCCA 
GGCCACAGCC CTAAGGCAGA GGGGGCCCAA ATCCCACTTG TCTCTGTTGT CACCAAGGCA 
AAATTGCCTG AAGAGAACCT TTTCCCCAAG CCTTTGCTCC CCAAAGTGAA GCCCAAGGTG 
GAAGTGCCCT GCAGTCAGAG AGCTGCATGC TCCCCCATCC GTTCTCAGCC AGTGGCTCTG 
CCAGAAGCCA GGACACCTAC CTCCCCAGTG AGCTCGCAGT GCCTGTCGCC TTTGGCCACC 
TCCACCCCCA CCAGCACCCA GCTCCCCATT TGCTCCCAGC CTCAGCCTTC CTCTGAGACC 
ACGGCCCCAT CCCCCACCAA GTCCCCCATA CGGCTCCAGC CTGTTTCAGC CAAAACATCT 
ATCCCAGTGG TTCCCCTCCC CTTGAAGAAC CAAGGGGACA CCAAGGACAG GCTGGGCAGC 
CCTCTCGCTG TGGATGAGGC CCTGAAGCGG AATGACCTGG TGGCAGAGTT CTGGATGAAG 
AGCGCCGAGA TCCGCCGCAG CCTCGGGCTC ACACCAGTGG ATCGGAGCAA GGTGTATGAG 
CCCAGCTTCC CTTCGCCTGC CTTTAAACCA GTGTCCCTAA AATCCTATGC AGTGGAGAAG 
TCTCCCGAGG GGGAGGGACT CCAGCTTCTG AAGCCTCCAC CTGTTCCTAA GAGACTAGGC 
CTGCCGAAGT CAGACGGTGA CCAGCCCTTC CTGCCAACCC CCAAGTCCCC GTCGGACAGA 
GAGCTGAAAA GCTCCCAGGA GGAGAGGAGA GATCTGTCGA GCAGCTCCGG GCTGGGCCTT 
CACGGGAGCT CCTCCAACAT GAAGACTTTG GGCAGCCAGA GCTTCAACAC ATCAGACTCC 
ACCATGCTCA CTCCCCCTTC AAGCCCACCG CCACCACCAC CCCAAGACGA GGAACCCGCA 
ACTCTTCGGA GGAAGCCATA TCAGACTCAT GAGCATAAGG ACGCTGAGCC CAAGGCCTCG 
GTGATTCCAC CACCACCACC TGCGACGTTC ATGCGGGCCC CCCGGGAGCC CACCCAGCCC 
CTCCAAGAGG AGATGCGGAA GTCGTTTGTG GAGAGCGTGG ATGAGATCCC ATTTGCCGAT 
GATGTGGAGG ACACCTATGA TGACAAGACC GAGGACTCGA GTCTCCAGGA GAAGTTCTTC 
ACACCCCCCT CCTACTGGCC CCGCACGGAG AGGCCCCTCC ACCCACCCCT AGCCAAGGAG 
AATGGTAGGT TGCCTACTCT GGAGAGTGGG GTCCAGCCAC AGAAGAGGGG CCTGCCCTTA 
GTCTCTCCAG AAGCCAAGGA GCTGGCTGAG GAGCGCATGC GAGCCAGGGA GAAGTCTGTG 
AAGAGCCAGG CGTTGAGAGA CGCTATGGCC AAGCAGCTGA GTAAGATGAA GGAAATGGAG 
ATGGCGGCTG GGACCTCCAG TCCCCCAGGA GGCACCTCCC ACAAAGCCTC CTCTGTTCCC 
TCCAAGGGCA AAGACCTCAG CCCCGAGTCC CCTAGACGCC CAGTTCTCAA AGGCCCCAGG 
GAGCCTGCCC TGAAGCACGA AGCCACTAGT GAGGAGGTCC TCTCCCCACC ATCGGACTCA 
GGGGGCCCAG ATGGTTCGGT CACCTCATCC GAGGGCTCCA GTGGGAAGAG CAAGAAGAGA 
TCGTCACTCT TCTCCCCCCG CAGAAACAAG AAAGAGAAGA AGTCCAAAGG TGAGGGCCGG 
CCCCCGGAGA AGCCCAGCCC AAGCCTTCTG GAGGAAGCTG CTGCCAAGCC CAAGTCCCTG 
TGGAAGTCGG TCTTTTCCGG GTACAAGAAG GACAAAAAGA AGAAGGGTGA TGACAAGTCC 
TGTTCCAGCA CCCCTTCCAG CGGTGCCACT GTGGACTCTG GCAAGCACAA GATGTCCCCT 
GTTGTGAGAG CAGAGCTGCA CCTGCGGCGC CAGTTGAGCT TCTCCGAGGA CTCAGACCTG 
TCCAGTGATG ACATCCTTGA GAGGTCCTCC CAGAAGTCCA GGCGAGAGTC GGTTTATGTA 
CCACACGCCT TGGCATTCAA GAGATCATAT CCATCAAAGC CGAGAACCTA CACAGAGGAG 
GAACTGAATG CCAAGCTGAC CCGGCGTGTG CAGAAGGCAG CTCGTAGACA GGCCAAGCAA 
GAGGAGCTGA AGCGGCTGCA CCGAGCACAG ATCATCCAGA GGCAGCTGGA GCAGGTGGAG 
GAGAAGCAGA GACAGCTGGA GGAAAGAGGG GTGGCCGTGG AGAAGGCGCT CCGTGGTGAA 
GCAGACTATT GGGGAGAGTC TTATTACAGT GACCTCATCG ACTTGCATCT GGGTGTTGAG 
CCCAGTGGCG GGACTCCACG GCGTAGACCT CTCTCCTTCT GCCCTTGCTG TGTCCAGGAA 
GGCATGGGCA AGAAGGATGA CCCCAAGCTG ATGCAGGAGT GGTTCAAGTT GGTGCAAGAG 
AAGAATGCCA TGGTGCGCTA TGAGTCGGAG CTGATGATTT TTGCCCGGGA GCTGGAACTG 
GAGGACCGGC AGAGCCGACT GCAGCAGGAG CTCCGGGAGC GGATGGCGGT GGAAGATCAC 
CTGAAGACTG AGGAGGAGCT GTCTGAGGAG AAGAAGATCT TGAACGAGAT GCTGGAGGTG 
GTGGAGCAGA GAGATGCCCT CGTGGCCCTG CTTGAGGAGC AGCGACTCCG GGAGAAGGAG 
GAGGACAAAG ACCTGGAGGC TGTCATGCTG TCCAAGGGCT TCAGCCTGAA CTGGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_022410891.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022266599.1 Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X22, mRNA.

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CloneID	OCe282687
Clone ID Related Accession (Same CDS sequence)	XM_022410891.1
Accession Version	XM_022410891.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	F-actin-methionine sulfoxide oxidase MICAL3 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGGGCTC GATAAAGAAG 
GAGTTCCCAC AGAACTTAGG AGGCAGCGAC ACGTGCTATT TCTGCCGGAA GCGGGTCTAC 
GTGATGGAAA GGCTGAGTGC TGAGGGCAAG TTCTTCCATC GGAGCTGCTT CAAGTGCGAG 
TACTGCGCCA CCACCCTGCG CCTCTCGGCC TATGCCTATG ACATTGAGGA CGGTAAATTC 
TACTGCAAAC CACACTACTG TTATCGACTC TCTGGCTCTG CACAGAGGAA GAGACCAGCA 
GTGGCTCCTC TCTCTGGAAA GGAGGCCAGA GAACCCCTGC AGGATGGCCC CACTGTGGAC 
ACAAATGGAC GGGCCAGCGC TGTCACCAGC CCTGCTGAGA GAACTCCAGG TTCAAGCGTG 
AATGGTGTGG AAGAGCCTAG CATTGCCAAG AGGCTCAGGG GCACTCCCGA GCGGATTGAG 
CTGGAGAACT ACCGCCTGTC CGTGAAGCAG GCCGAGGAGC TGGAGGAGGT GCCCGAGGAG 
ACACAGGCCG AGCACAACCT GAGCAGCGTG CTGGACACGG GGGCAGAGGA GGACGTGGCC 
AGCAGCAGTT CTGAGTCAGA GATGGAGGAA GAGGAGGAAG AAGAGCCTCC TCTGCCCACT 
TCGGACCTGG GCGGTGTTCC TTGGAAGGAG GCCGTGAGGA TCCATGCTCT TCTGAAAGGA 
AAGAGTGAAG AGGAGCTAGA GGCCTCAAAG AGCTTTGGGC CTGGGCATGA AGAAGACGAG 
GAGGAGGAGG AGTATGAAGA GGAGGAGGAG GAGGAGGAGG AAGAATATGA AGAGGAAGAG 
GAAGAGGAAG AGTCTAGTGA AGAAGGGGAG TATTGCCCCT GGGACAGAGA ACTCCTACAA 
GGCCAGTGGC TCCGGCAGCT GTCACAGGAA GAAACTACGG GTACACGTAA AGTTGGGAGC 
CAGAGGCTAC AGCAGATCGT AAATCCGGCC GACCCCTTGG AGATCCAGGC TGATGTGCAC 
TGGACACATA TCCGAGAGAG AGAGGAGGAG GAGCGAATGG TCCCAACCTC TGAGTCCTCC 
ACTTCTAGAG TGCCTGACCT TCCCTCCATA CGCCGCGCAG CAGTGCAGGC CTGGCTGGAG 
ACGGTCTCTG GAGTCCCATT TGATGAGAAT GACCTGGAGG AAGACGTGGA CTCGGAGCCA 
GCAAAGATAG AAGGGGAGGC CACAGAGAAT GGGGACACAG GGGACACTGG TGCTGAGCTG 
GACGATGATC AGCGCTGGTC TGACGGGATC CCATCGGATG CCGAGACGGA TCTCCGCGTG 
CAGCACCAGG AGGCGGTAGA GGCAGACCTG GAGCTGAGGG TCTCAGAGAA TGAGGAGGAG 
CCACCTGAGG TCACACCAGG GCATCCGGAG AGAAGTCTCA CCCGGGTCCC CAGCCCTGCC 
CTGTCCCCTG AGGAGCAAGC TGGTCTGCGG TCCCCAGGCC ACAGCCCTAA GGCAGAGGGG 
GCCCAAATCC CACTTGTCTC TGTTGTCACC AAGGCAAAAT TGCCTGAAGA GAACCTTTTC 
CCCAAGCCTT TGCTCCCCAA AGTGAAGCCC AAGGTGGAAG TGCCCTGCAG TCAGAGAGCT 
GCATGCTCCC CCATCCGTTC TCAGCCAGTG GCTCTGCCAG AAGCCAGGAC ACCTACCTCC 
CCAGTGAGCT CGCAGTGCCT GTCGCCTTTG GCCACCTCCA CCCCCACCAG CACCCAGCTC 
CCCATTTGCT CCCAGCCTCA GCCTTCCTCT GAGACCACGG CCCCATCCCC CACCAAGTCC 
CCCATACGGC TCCAGCCTGT TTCAGCCAAA ACATCTATCC CAGTGGTTCC CCTCCCCTTG 
AAGAACCAAG GGGACACCAA GGACAGGCTG GGCAGCCCTC TCGCTGTGGA TGAGGCCCTG 
AAGCGGAATG ACCTGGTGGC AGAGTTCTGG ATGAAGAGCG CCGAGATCCG CCGCAGCCTC 
GGGCTCACAC CAGTGGATCG GAGCAAGGTG TATGAGCCCA GCTTCCCTTC GCCTGCCTTT 
AAACCAGTGT CCCTAAAATC CTATGCAGTG GAGAAGTCTC CCGAGGGGGA GGGACTCCAG 
CTTCTGAAGC CTCCACCTGT TCCTAAGAGA CTAGGCCTGC CGAAGTCAGA CGGTGACCAG 
CCCTTCCTGC CAACCCCCAA GTCCCCGTCG GACAGAGAGC TGAAAAGCTC CCAGGAGGAG 
AGGAGAGATC TGTCGAGCAG CTCCGGGCTG GGCCTTCACG GGAGCTCCTC CAACATGAAG 
ACTTTGGGCA GCCAGAGCTT CAACACATCA GACTCCACCA TGCTCACTCC CCCTTCAAGC 
CCACCGCCAC CACCACCCCA AGACGAGGAA CCCGCAACTC TTCGGAGGAA GCCATATCAG 
ACTCATGAGC ATAAGGACGC TGAGCCCAAG GCCTCGGTGA TTCCACCACC ACCACCTGCG 
ACGTTCATGC GGGCCCCCCG GGAGCCCACC CAGCCCCTCC AAGAGGAGAT GCGGAAGTCG 
TTTGTGGAGA GCGTGGATGA GATCCCATTT GCCGATGATG TGGAGGACAC CTATGATGAC 
AAGACCGAGG ACTCGAGTCT CCAGGAGAAG TTCTTCACAC CCCCCTCCTA CTGGCCCCGC 
ACGGAGAGGC CCCTCCACCC ACCCCTAGCC AAGGAGAATG GTAGGTTGCC TACTCTGGAG 
AGTGGGGTCC AGCCACAGAA GAGGGGCCTG CCCTTAGTCT CTCCAGAAGC CAAGGAGCTG 
GCTGAGGAGC GCATGCGAGC CAGGGAGAAG TCTGTGAAGA GCCAGGCGTT GAGAGACGCT 
ATGGCCAAGC AGCTGAGTAA GATGAAGGAA ATGGAGATGG CGGCTGGGAC CTCCAGTCCC 
CCAGGAGGCA CCTCCCACAA AGCCTCCTCT GTTCCCTCCA AGGGCAAAGA CCTCAGCCCC 
GAGTCCCCTA GACGCCCAGT TCTCAAAGGC CCCAGGGAGC CTGCCCTGAA GCACGAAGCC 
ACTAGTGAGG AGGTCCTCTC CCCACCATCG GACTCAGGGG GCCCAGATGG TTCGGTCACC 
TCATCCGAGG GCTCCAGTGG GAAGAGCAAG AAGAGATCGT CACTCTTCTC CCCCCGCAGA 
AACAAGAAAG AGAAGAAGTC CAAAGGTGAG GGCCGGCCCC CGGAGAAGCC CAGCCCAAGC 
CTTCTGGAGG AAGCTGCTGC CAAGCCCAAG TCCCTGTGGA AGTCGGTCTT TTCCGGGTAC 
AAGAAGGACA AAAAGAAGAA GGGTGATGAC AAGTCCTGTT CCAGCACCCC TTCCAGCGGT 
GCCACTGTGG ACTCTGGCAA GCACAAGATG TCCCCTGTTG TGAGAGCAGA GCTGCACCTG 
CGGCGCCAGT TGAGCTTCTC CGAGGACTCA GACCTGTCCA GTGATGACAT CCTTGAGAGG 
TCCTCCCAGA AGTCCAGGCG AGAGTCGGTT TATGTACCAC ACGCCTTGGC ATTCAAGAGA 
TCATATCCAT CAAAGCCGAG AACCTACACA GAGGAGGAAC TGAATGCCAA GCTGACCCGG 
CGTGTGCAGA AGGCAGCTCG TAGACAGGCC AAGCAAGAGG AGCTGAAGCG GCTGCACCGA 
GCACAGATCA TCCAGAGGCA GCTGGAGCAG GTGGAGGAGA AGCAGAGACA GCTGGAGGAA 
AGAGGGGTGG CCGTGGAGAA GGCGCTCCGT GGTGAAGCAG ACTATTGGGG AGAGTCTTAT 
TACAGTGACC TCATCGACTT GCATCTGGGT GTTGAGCCCA GTGGCGGGAC TCCACGGCGT 
AGACCTCTCT CCTTCTGCCC TTGCTGTGTC CAGGAAGGCA TGGGCAAGAA GGATGACCCC 
AAGCTGATGC AGGAGTGGTT CAAGTTGGTG CAAGAGAAGA ATGCCATGGT GCGCTATGAG 
TCGGAGCTGA TGATTTTTGC CCGGGAGCTG GAACTGGAGG ACCGGCAGAG CCGACTGCAG 
CAGGAGCTCC GGGAGCGGAT GGCGGTGGAA GATCACCTGA AGACTGAGGA GGAGCTGTCT 
GAGGAGAAGA AGATCTTGAA CGAGATGCTG GAGGTGGTGG AGCAGAGAGA TGCCCTCGTG 
GCCCTGCTTG AGGAGCAGCG ACTCCGGGAG AAGGAGGAGG ACAAAGACCT GGAGGCTGTC 
ATGCTGTCCA AGGGCTTCAG CCTGAACTGG TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022266599.1
CDS	258..6713
Translation	MEDSKNETVNHAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDYRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKACTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFQGLVQPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELKEATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLNYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREQNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNININFLRPSQVRHLYNTGETRDIHLEMENLVNSRTTPKLTRNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGKEMASVGEPDKLSMVMYLTQFYEMFKDSLPSSDALDLNAEEKAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEEAPRGYRGGRPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSTGMRRQPTQERGVSQPSCCLPEQGHPAPTPQWKQGSIKKEFPQNLGGSDTCYFCRKRVYVMERLSAEGKFFHRSCFKCEYCATTLRLSAYAYDIEDGKFYCKPHYCYRLSGSAQRKRPAVAPLSGKEAREPLQDGPTVDTNGRASAVTSPAERTPGSSVNGVEEPSIAKRLRGTPERIELENYRLSVKQAEELEEVPEETQAEHNLSSVLDTGAEEDVASSSSESEMEEEEEEEPPLPTSDLGGVPWKEAVRIHALLKGKSEEELEASKSFGPGHEEDEEEEEYEEEEEEEEEEYEEEEEEEESSEEGEYCPWDRELLQGQWLRQLSQEETTGTRKVGSQRLQQIVNPADPLEIQADVHWTHIREREEEERMVPTSESSTSRVPDLPSIRRAAVQAWLETVSGVPFDENDLEEDVDSEPAKIEGEATENGDTGDTGAELDDDQRWSDGIPSDAETDLRVQHQEAVEADLELRVSENEEEPPEVTPGHPERSLTRVPSPALSPEEQAGLRSPGHSPKAEGAQIPLVSVVTKAKLPEENLFPKPLLPKVKPKVEVPCSQRAACSPIRSQPVALPEARTPTSPVSSQCLSPLATSTPTSTQLPICSQPQPSSETTAPSPTKSPIRLQPVSAKTSIPVVPLPLKNQGDTKDRLGSPLAVDEALKRNDLVAEFWMKSAEIRRSLGLTPVDRSKVYEPSFPSPAFKPVSLKSYAVEKSPEGEGLQLLKPPPVPKRLGLPKSDGDQPFLPTPKSPSDRELKSSQEERRDLSSSSGLGLHGSSSNMKTLGSQSFNTSDSTMLTPPSSPPPPPPQDEEPATLRRKPYQTHEHKDAEPKASVIPPPPPATFMRAPREPTQPLQEEMRKSFVESVDEIPFADDVEDTYDDKTEDSSLQEKFFTPPSYWPRTERPLHPPLAKENGRLPTLESGVQPQKRGLPLVSPEAKELAEERMRAREKSVKSQALRDAMAKQLSKMKEMEMAAGTSSPPGGTSHKASSVPSKGKDLSPESPRRPVLKGPREPALKHEATSEEVLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRNKKEKKSKGEGRPPEKPSPSLLEEAAAKPKSLWKSVFSGYKKDKKKKGDDKSCSSTPSSGATVDSGKHKMSPVVRAELHLRRQLSFSEDSDLSSDDILERSSQKSRRESVYVPHALAFKRSYPSKPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELSEEKKILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAVMLSKGFSLNWS

Target ORF information:

RefSeq Version	XM_022410891.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022410891.1

ORF Insert Sequence:

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGGGCTC GATAAAGAAG 
GAGTTCCCAC AGAACTTAGG AGGCAGCGAC ACGTGCTATT TCTGCCGGAA GCGGGTCTAC 
GTGATGGAAA GGCTGAGTGC TGAGGGCAAG TTCTTCCATC GGAGCTGCTT CAAGTGCGAG 
TACTGCGCCA CCACCCTGCG CCTCTCGGCC TATGCCTATG ACATTGAGGA CGGTAAATTC 
TACTGCAAAC CACACTACTG TTATCGACTC TCTGGCTCTG CACAGAGGAA GAGACCAGCA 
GTGGCTCCTC TCTCTGGAAA GGAGGCCAGA GAACCCCTGC AGGATGGCCC CACTGTGGAC 
ACAAATGGAC GGGCCAGCGC TGTCACCAGC CCTGCTGAGA GAACTCCAGG TTCAAGCGTG 
AATGGTGTGG AAGAGCCTAG CATTGCCAAG AGGCTCAGGG GCACTCCCGA GCGGATTGAG 
CTGGAGAACT ACCGCCTGTC CGTGAAGCAG GCCGAGGAGC TGGAGGAGGT GCCCGAGGAG 
ACACAGGCCG AGCACAACCT GAGCAGCGTG CTGGACACGG GGGCAGAGGA GGACGTGGCC 
AGCAGCAGTT CTGAGTCAGA GATGGAGGAA GAGGAGGAAG AAGAGCCTCC TCTGCCCACT 
TCGGACCTGG GCGGTGTTCC TTGGAAGGAG GCCGTGAGGA TCCATGCTCT TCTGAAAGGA 
AAGAGTGAAG AGGAGCTAGA GGCCTCAAAG AGCTTTGGGC CTGGGCATGA AGAAGACGAG 
GAGGAGGAGG AGTATGAAGA GGAGGAGGAG GAGGAGGAGG AAGAATATGA AGAGGAAGAG 
GAAGAGGAAG AGTCTAGTGA AGAAGGGGAG TATTGCCCCT GGGACAGAGA ACTCCTACAA 
GGCCAGTGGC TCCGGCAGCT GTCACAGGAA GAAACTACGG GTACACGTAA AGTTGGGAGC 
CAGAGGCTAC AGCAGATCGT AAATCCGGCC GACCCCTTGG AGATCCAGGC TGATGTGCAC 
TGGACACATA TCCGAGAGAG AGAGGAGGAG GAGCGAATGG TCCCAACCTC TGAGTCCTCC 
ACTTCTAGAG TGCCTGACCT TCCCTCCATA CGCCGCGCAG CAGTGCAGGC CTGGCTGGAG 
ACGGTCTCTG GAGTCCCATT TGATGAGAAT GACCTGGAGG AAGACGTGGA CTCGGAGCCA 
GCAAAGATAG AAGGGGAGGC CACAGAGAAT GGGGACACAG GGGACACTGG TGCTGAGCTG 
GACGATGATC AGCGCTGGTC TGACGGGATC CCATCGGATG CCGAGACGGA TCTCCGCGTG 
CAGCACCAGG AGGCGGTAGA GGCAGACCTG GAGCTGAGGG TCTCAGAGAA TGAGGAGGAG 
CCACCTGAGG TCACACCAGG GCATCCGGAG AGAAGTCTCA CCCGGGTCCC CAGCCCTGCC 
CTGTCCCCTG AGGAGCAAGC TGGTCTGCGG TCCCCAGGCC ACAGCCCTAA GGCAGAGGGG 
GCCCAAATCC CACTTGTCTC TGTTGTCACC AAGGCAAAAT TGCCTGAAGA GAACCTTTTC 
CCCAAGCCTT TGCTCCCCAA AGTGAAGCCC AAGGTGGAAG TGCCCTGCAG TCAGAGAGCT 
GCATGCTCCC CCATCCGTTC TCAGCCAGTG GCTCTGCCAG AAGCCAGGAC ACCTACCTCC 
CCAGTGAGCT CGCAGTGCCT GTCGCCTTTG GCCACCTCCA CCCCCACCAG CACCCAGCTC 
CCCATTTGCT CCCAGCCTCA GCCTTCCTCT GAGACCACGG CCCCATCCCC CACCAAGTCC 
CCCATACGGC TCCAGCCTGT TTCAGCCAAA ACATCTATCC CAGTGGTTCC CCTCCCCTTG 
AAGAACCAAG GGGACACCAA GGACAGGCTG GGCAGCCCTC TCGCTGTGGA TGAGGCCCTG 
AAGCGGAATG ACCTGGTGGC AGAGTTCTGG ATGAAGAGCG CCGAGATCCG CCGCAGCCTC 
GGGCTCACAC CAGTGGATCG GAGCAAGGTG TATGAGCCCA GCTTCCCTTC GCCTGCCTTT 
AAACCAGTGT CCCTAAAATC CTATGCAGTG GAGAAGTCTC CCGAGGGGGA GGGACTCCAG 
CTTCTGAAGC CTCCACCTGT TCCTAAGAGA CTAGGCCTGC CGAAGTCAGA CGGTGACCAG 
CCCTTCCTGC CAACCCCCAA GTCCCCGTCG GACAGAGAGC TGAAAAGCTC CCAGGAGGAG 
AGGAGAGATC TGTCGAGCAG CTCCGGGCTG GGCCTTCACG GGAGCTCCTC CAACATGAAG 
ACTTTGGGCA GCCAGAGCTT CAACACATCA GACTCCACCA TGCTCACTCC CCCTTCAAGC 
CCACCGCCAC CACCACCCCA AGACGAGGAA CCCGCAACTC TTCGGAGGAA GCCATATCAG 
ACTCATGAGC ATAAGGACGC TGAGCCCAAG GCCTCGGTGA TTCCACCACC ACCACCTGCG 
ACGTTCATGC GGGCCCCCCG GGAGCCCACC CAGCCCCTCC AAGAGGAGAT GCGGAAGTCG 
TTTGTGGAGA GCGTGGATGA GATCCCATTT GCCGATGATG TGGAGGACAC CTATGATGAC 
AAGACCGAGG ACTCGAGTCT CCAGGAGAAG TTCTTCACAC CCCCCTCCTA CTGGCCCCGC 
ACGGAGAGGC CCCTCCACCC ACCCCTAGCC AAGGAGAATG GTAGGTTGCC TACTCTGGAG 
AGTGGGGTCC AGCCACAGAA GAGGGGCCTG CCCTTAGTCT CTCCAGAAGC CAAGGAGCTG 
GCTGAGGAGC GCATGCGAGC CAGGGAGAAG TCTGTGAAGA GCCAGGCGTT GAGAGACGCT 
ATGGCCAAGC AGCTGAGTAA GATGAAGGAA ATGGAGATGG CGGCTGGGAC CTCCAGTCCC 
CCAGGAGGCA CCTCCCACAA AGCCTCCTCT GTTCCCTCCA AGGGCAAAGA CCTCAGCCCC 
GAGTCCCCTA GACGCCCAGT TCTCAAAGGC CCCAGGGAGC CTGCCCTGAA GCACGAAGCC 
ACTAGTGAGG AGGTCCTCTC CCCACCATCG GACTCAGGGG GCCCAGATGG TTCGGTCACC 
TCATCCGAGG GCTCCAGTGG GAAGAGCAAG AAGAGATCGT CACTCTTCTC CCCCCGCAGA 
AACAAGAAAG AGAAGAAGTC CAAAGGTGAG GGCCGGCCCC CGGAGAAGCC CAGCCCAAGC 
CTTCTGGAGG AAGCTGCTGC CAAGCCCAAG TCCCTGTGGA AGTCGGTCTT TTCCGGGTAC 
AAGAAGGACA AAAAGAAGAA GGGTGATGAC AAGTCCTGTT CCAGCACCCC TTCCAGCGGT 
GCCACTGTGG ACTCTGGCAA GCACAAGATG TCCCCTGTTG TGAGAGCAGA GCTGCACCTG 
CGGCGCCAGT TGAGCTTCTC CGAGGACTCA GACCTGTCCA GTGATGACAT CCTTGAGAGG 
TCCTCCCAGA AGTCCAGGCG AGAGTCGGTT TATGTACCAC ACGCCTTGGC ATTCAAGAGA 
TCATATCCAT CAAAGCCGAG AACCTACACA GAGGAGGAAC TGAATGCCAA GCTGACCCGG 
CGTGTGCAGA AGGCAGCTCG TAGACAGGCC AAGCAAGAGG AGCTGAAGCG GCTGCACCGA 
GCACAGATCA TCCAGAGGCA GCTGGAGCAG GTGGAGGAGA AGCAGAGACA GCTGGAGGAA 
AGAGGGGTGG CCGTGGAGAA GGCGCTCCGT GGTGAAGCAG ACTATTGGGG AGAGTCTTAT 
TACAGTGACC TCATCGACTT GCATCTGGGT GTTGAGCCCA GTGGCGGGAC TCCACGGCGT 
AGACCTCTCT CCTTCTGCCC TTGCTGTGTC CAGGAAGGCA TGGGCAAGAA GGATGACCCC 
AAGCTGATGC AGGAGTGGTT CAAGTTGGTG CAAGAGAAGA ATGCCATGGT GCGCTATGAG 
TCGGAGCTGA TGATTTTTGC CCGGGAGCTG GAACTGGAGG ACCGGCAGAG CCGACTGCAG 
CAGGAGCTCC GGGAGCGGAT GGCGGTGGAA GATCACCTGA AGACTGAGGA GGAGCTGTCT 
GAGGAGAAGA AGATCTTGAA CGAGATGCTG GAGGTGGTGG AGCAGAGAGA TGCCCTCGTG 
GCCCTGCTTG AGGAGCAGCG ACTCCGGGAG AAGGAGGAGG ACAAAGACCT GGAGGCTGTC 
ATGCTGTCCA AGGGCTTCAG CCTGAACTGG TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_022410892.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022266600.1 Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X23, mRNA.

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CloneID	OCe282688
Clone ID Related Accession (Same CDS sequence)	XM_022410892.1
Accession Version	XM_022410892.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	F-actin-methionine sulfoxide oxidase MICAL3 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTGCCTGAC CTTCCCTCCA TACGCCGCGC AGCAGTGCAG 
GCCTGGCTGG AGACGGTCTC TGGAGTCCCA TTTGATGAGA ATGACCTGGA GGAAGACGTG 
GACTCGGAGC CAGCAAAGAT AGAAGGGGAG GCCACAGAGA ATGGGGACAC AGGGGACACT 
GGTGCTGAGC TGGACGATGA TCAGCGCTGG TCTGACGGGA TCCCATCGGA TGCCGAGACG 
GATCTCCGCG TGCAGCACCA GGAGGCGGTA GAGGCAGACC TGGAGCTGAG GGTCTCAGAG 
AATGAGGAGG AGCCACCTGA GGTCACACCA GGGCATCCGG AGAGAAGTCT CACCCGGGTC 
CCCAGCCCTG CCCTGTCCCC TGAGGAGCAA GCTGGTCTGC GGTCCCCAGG CCACAGCCCT 
AAGGCAGAGG GGGCCCAAAT CCCACTTGTC TCTGTTGTCA CCAAGGCAAA ATTGCCTGAA 
GAGAACCTTT TCCCCAAGCC TTTGCTCCCC AAAGTGAAGC CCAAGGTGGA AGTGCCCTGC 
AGTCAGAGAG CTGCATGCTC CCCCATCCGT TCTCAGCCAG TGGCTCTGCC AGAAGCCAGG 
ACACCTACCT CCCCAGTGAG CTCGCAGTGC CTGTCGCCTT TGGCCACCTC CACCCCCACC 
AGCACCCAGC TCCCCATTTG CTCCCAGCCT CAGCCTTCCT CTGAGACCAC GGCCCCATCC 
CCCACCAAGT CCCCCATACG GCTCCAGCCT GTTTCAGCCA AAACATCTAT CCCAGTGGTT 
CCCCTCCCCT TGAAGAACCA AGGGGACACC AAGGACAGGC TGGGCAGCCC TCTCGCTGTG 
GATGAGGCCC TGAAGCGGAA TGACCTGGTG GCAGAGTTCT GGATGAAGAG CGCCGAGATC 
CGCCGCAGCC TCGGGCTCAC ACCAGTGGAT CGGAGCAAGG TGTATGAGCC CAGCTTCCCT 
TCGCCTGCCT TTAAACCAGT GTCCCTAAAA TCCTATGCAG TGGAGAAGTC TCCCGAGGGG 
GAGGGACTCC AGCTTCTGAA GCCTCCACCT GTTCCTAAGA GACTAGGCCT GCCGAAGTCA 
GACGGTGACC AGCCCTTCCT GCCAACCCCC AAGTCCCCGT CGGACAGAGA GCTGAAAAGC 
TCCCAGGAGG AGAGGAGAGA TCTGTCGAGC AGCTCCGGGC TGGGCCTTCA CGGGAGCTCC 
TCCAACATGA AGACTTTGGG CAGCCAGAGC TTCAACACAT CAGACTCCAC CATGCTCACT 
CCCCCTTCAA GCCCACCGCC ACCACCACCC CAAGACGAGG AACCCGCAAC TCTTCGGAGG 
AAGCCATATC AGACTCATGA GCATAAGGAC GCTGAGCCCA AGGCCTCGGT GATTCCACCA 
CCACCACCTG CGACGTTCAT GCGGGCCCCC CGGGAGCCCA CCCAGCCCCT CCAAGAGGAG 
ATGCGGAAGT CGTTTGTGGA GAGCGTGGAT GAGATCCCAT TTGCCGATGA TGTGGAGGAC 
ACCTATGATG ACAAGACCGA GGACTCGAGT CTCCAGGAGA AGTTCTTCAC ACCCCCCTCC 
TACTGGCCCC GCACGGAGAG GCCCCTCCAC CCACCCCTAG CCAAGGAGAA TGGTAGGTTG 
CCTACTCTGG AGAGTGGGGT CCAGCCACAG AAGAGGGGCC TGCCCTTAGT CTCTCCAGAA 
GCCAAGGAGC TGGCTGAGGA GCGCATGCGA GCCAGGGAGA AGTCTGTGAA GAGCCAGGCG 
TTGAGAGACG CTATGGCCAA GCAGCTGAGT AAGATGAAGG AAATGGAGAT GGCGGCTGGG 
ACCTCCAGTC CCCCAGGAGG CACCTCCCAC AAAGCCTCCT CTGTTCCCTC CAAGGGCAAA 
GACCTCAGCC CCGAGTCCCC TAGACGCCCA GTTCTCAAAG GCCCCAGGGA GCCTGCCCTG 
AAGCACGAAG CCACTAGTGA GGAGGTCCTC TCCCCACCAT CGGACTCAGG GGGCCCAGAT 
GGTTCGGTCA CCTCATCCGA GGGCTCCAGT GGGAAGAGCA AGAAGAGATC GTCACTCTTC 
TCCCCCCGCA GAAACAAGAA AGAGAAGAAG TCCAAAGGTG AGGGCCGGCC CCCGGAGAAG 
CCCAGCCCAA GCCTTCTGGA GGAAGCTGCT GCCAAGCCCA AGTCCCTGTG GAAGTCGGTC 
TTTTCCGGGT ACAAGAAGGA CAAAAAGAAG AAGGGTGATG ACAAGTCCTG TTCCAGCACC 
CCTTCCAGCG GTGCCACTGT GGACTCTGGC AAGCACAAGA TGTCCCCTGT TGTGAGAGCA 
GAGCTGCACC TGCGGCGCCA GTTGAGCTTC TCCGAGGACT CAGACCTGTC CAGTGATGAC 
ATCCTTGAGA GGTCCTCCCA GAAGTCCAGG CGAGAGTCGG TTTATGTACC ACACGCCTTG 
GCATTCAAGA GATCATATCC ATCAAAGCCG AGAACCTACA CAGAGGAGGA ACTGAATGCC 
AAGCTGACCC GGCGTGTGCA GAAGGCAGCT CGTAGACAGG CCAAGCAAGA GGAGCTGAAG 
CGGCTGCACC GAGCACAGAT CATCCAGAGG CAGCTGGAGC AGGTGGAGGA GAAGCAGAGA 
CAGCTGGAGG AAAGAGGGGT GGCCGTGGAG AAGGCGCTCC GTGGTGAAGC AGATTACTCC 
TGCCTAGATG GAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022266600.1
CDS	258..6635
Translation	MEDSKNETVNHAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDYRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKACTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFQGLVQPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELKEATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLNYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREQNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNININFLRPSQVRHLYNTGETRDIHLEMENLVNSRTTPKLTRNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGKEMASVGEPDKLSMVMYLTQFYEMFKDSLPSSDALDLNAEEKAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEEAPRGYRGGRPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSTGMRRQPTQERGVSQPSCCLPEQGHPAPTPQWKQQQEKERSRTCPKKVITLSPPLTPPPCRAPRSQQTYRDLDADSRGKQSPHHERPEPEPSRRFFVDQWELSLSLRSSNRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCRKRVYVMERLSAEGKFFHRSCFKCEYCATTLRLSAYAYDIEDGKFYCKPHYCYRLSGSAQRKRPAVAPLSGKEAREPLQDGPTVDTNGRASAVTSPAERTPGSSVNGVEEPSIAKRLRGTPERIELENYRLSVKQAEELEEVPEETQAEHNLSSVLDTGAEEDVASSSSESEMEEEEEEEPPLPTSDLGGVPWKEAVRIHALLKGKSEEELEASKSFGPGHEEDEEEEEYEEEEEEEEEEYEEEEEEEESSEEGEYCPWDRELLQGQWLRQLSQEETTGTRKVGSQRLQQIVNPADPLEIQADVHWTHIREREEEERMVPTSESSTSRVPDLPSIRRAAVQAWLETVSGVPFDENDLEEDVDSEPAKIEGEATENGDTGDTGAELDDDQRWSDGIPSDAETDLRVQHQEAVEADLELRVSENEEEPPEVTPGHPERSLTRVPSPALSPEEQAGLRSPGHSPKAEGAQIPLVSVVTKAKLPEENLFPKPLLPKVKPKVEVPCSQRAACSPIRSQPVALPEARTPTSPVSSQCLSPLATSTPTSTQLPICSQPQPSSETTAPSPTKSPIRLQPVSAKTSIPVVPLPLKNQGDTKDRLGSPLAVDEALKRNDLVAEFWMKSAEIRRSLGLTPVDRSKVYEPSFPSPAFKPVSLKSYAVEKSPEGEGLQLLKPPPVPKRLGLPKSDGDQPFLPTPKSPSDRELKSSQEERRDLSSSSGLGLHGSSSNMKTLGSQSFNTSDSTMLTPPSSPPPPPPQDEEPATLRRKPYQTHEHKDAEPKASVIPPPPPATFMRAPREPTQPLQEEMRKSFVESVDEIPFADDVEDTYDDKTEDSSLQEKFFTPPSYWPRTERPLHPPLAKENGRLPTLESGVQPQKRGLPLVSPEAKELAEERMRAREKSVKSQALRDAMAKQLSKMKEMEMAAGTSSPPGGTSHKASSVPSKGKDLSPESPRRPVLKGPREPALKHEATSEEVLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRNKKEKKSKGEGRPPEKPSPSLLEEAAAKPKSLWKSVFSGYKKDKKKKGDDKSCSSTPSSGATVDSGKHKMSPVVRAELHLRRQLSFSEDSDLSSDDILERSSQKSRRESVYVPHALAFKRSYPSKPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYSCLDGK

Target ORF information:

RefSeq Version	XM_022410892.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022410892.1

ORF Insert Sequence:

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTGCCTGAC CTTCCCTCCA TACGCCGCGC AGCAGTGCAG 
GCCTGGCTGG AGACGGTCTC TGGAGTCCCA TTTGATGAGA ATGACCTGGA GGAAGACGTG 
GACTCGGAGC CAGCAAAGAT AGAAGGGGAG GCCACAGAGA ATGGGGACAC AGGGGACACT 
GGTGCTGAGC TGGACGATGA TCAGCGCTGG TCTGACGGGA TCCCATCGGA TGCCGAGACG 
GATCTCCGCG TGCAGCACCA GGAGGCGGTA GAGGCAGACC TGGAGCTGAG GGTCTCAGAG 
AATGAGGAGG AGCCACCTGA GGTCACACCA GGGCATCCGG AGAGAAGTCT CACCCGGGTC 
CCCAGCCCTG CCCTGTCCCC TGAGGAGCAA GCTGGTCTGC GGTCCCCAGG CCACAGCCCT 
AAGGCAGAGG GGGCCCAAAT CCCACTTGTC TCTGTTGTCA CCAAGGCAAA ATTGCCTGAA 
GAGAACCTTT TCCCCAAGCC TTTGCTCCCC AAAGTGAAGC CCAAGGTGGA AGTGCCCTGC 
AGTCAGAGAG CTGCATGCTC CCCCATCCGT TCTCAGCCAG TGGCTCTGCC AGAAGCCAGG 
ACACCTACCT CCCCAGTGAG CTCGCAGTGC CTGTCGCCTT TGGCCACCTC CACCCCCACC 
AGCACCCAGC TCCCCATTTG CTCCCAGCCT CAGCCTTCCT CTGAGACCAC GGCCCCATCC 
CCCACCAAGT CCCCCATACG GCTCCAGCCT GTTTCAGCCA AAACATCTAT CCCAGTGGTT 
CCCCTCCCCT TGAAGAACCA AGGGGACACC AAGGACAGGC TGGGCAGCCC TCTCGCTGTG 
GATGAGGCCC TGAAGCGGAA TGACCTGGTG GCAGAGTTCT GGATGAAGAG CGCCGAGATC 
CGCCGCAGCC TCGGGCTCAC ACCAGTGGAT CGGAGCAAGG TGTATGAGCC CAGCTTCCCT 
TCGCCTGCCT TTAAACCAGT GTCCCTAAAA TCCTATGCAG TGGAGAAGTC TCCCGAGGGG 
GAGGGACTCC AGCTTCTGAA GCCTCCACCT GTTCCTAAGA GACTAGGCCT GCCGAAGTCA 
GACGGTGACC AGCCCTTCCT GCCAACCCCC AAGTCCCCGT CGGACAGAGA GCTGAAAAGC 
TCCCAGGAGG AGAGGAGAGA TCTGTCGAGC AGCTCCGGGC TGGGCCTTCA CGGGAGCTCC 
TCCAACATGA AGACTTTGGG CAGCCAGAGC TTCAACACAT CAGACTCCAC CATGCTCACT 
CCCCCTTCAA GCCCACCGCC ACCACCACCC CAAGACGAGG AACCCGCAAC TCTTCGGAGG 
AAGCCATATC AGACTCATGA GCATAAGGAC GCTGAGCCCA AGGCCTCGGT GATTCCACCA 
CCACCACCTG CGACGTTCAT GCGGGCCCCC CGGGAGCCCA CCCAGCCCCT CCAAGAGGAG 
ATGCGGAAGT CGTTTGTGGA GAGCGTGGAT GAGATCCCAT TTGCCGATGA TGTGGAGGAC 
ACCTATGATG ACAAGACCGA GGACTCGAGT CTCCAGGAGA AGTTCTTCAC ACCCCCCTCC 
TACTGGCCCC GCACGGAGAG GCCCCTCCAC CCACCCCTAG CCAAGGAGAA TGGTAGGTTG 
CCTACTCTGG AGAGTGGGGT CCAGCCACAG AAGAGGGGCC TGCCCTTAGT CTCTCCAGAA 
GCCAAGGAGC TGGCTGAGGA GCGCATGCGA GCCAGGGAGA AGTCTGTGAA GAGCCAGGCG 
TTGAGAGACG CTATGGCCAA GCAGCTGAGT AAGATGAAGG AAATGGAGAT GGCGGCTGGG 
ACCTCCAGTC CCCCAGGAGG CACCTCCCAC AAAGCCTCCT CTGTTCCCTC CAAGGGCAAA 
GACCTCAGCC CCGAGTCCCC TAGACGCCCA GTTCTCAAAG GCCCCAGGGA GCCTGCCCTG 
AAGCACGAAG CCACTAGTGA GGAGGTCCTC TCCCCACCAT CGGACTCAGG GGGCCCAGAT 
GGTTCGGTCA CCTCATCCGA GGGCTCCAGT GGGAAGAGCA AGAAGAGATC GTCACTCTTC 
TCCCCCCGCA GAAACAAGAA AGAGAAGAAG TCCAAAGGTG AGGGCCGGCC CCCGGAGAAG 
CCCAGCCCAA GCCTTCTGGA GGAAGCTGCT GCCAAGCCCA AGTCCCTGTG GAAGTCGGTC 
TTTTCCGGGT ACAAGAAGGA CAAAAAGAAG AAGGGTGATG ACAAGTCCTG TTCCAGCACC 
CCTTCCAGCG GTGCCACTGT GGACTCTGGC AAGCACAAGA TGTCCCCTGT TGTGAGAGCA 
GAGCTGCACC TGCGGCGCCA GTTGAGCTTC TCCGAGGACT CAGACCTGTC CAGTGATGAC 
ATCCTTGAGA GGTCCTCCCA GAAGTCCAGG CGAGAGTCGG TTTATGTACC ACACGCCTTG 
GCATTCAAGA GATCATATCC ATCAAAGCCG AGAACCTACA CAGAGGAGGA ACTGAATGCC 
AAGCTGACCC GGCGTGTGCA GAAGGCAGCT CGTAGACAGG CCAAGCAAGA GGAGCTGAAG 
CGGCTGCACC GAGCACAGAT CATCCAGAGG CAGCTGGAGC AGGTGGAGGA GAAGCAGAGA 
CAGCTGGAGG AAAGAGGGGT GGCCGTGGAG AAGGCGCTCC GTGGTGAAGC AGATTACTCC 
TGCCTAGATG GAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_022410893.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022266601.1 Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X24, mRNA.

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CloneID	OCe282689
Clone ID Related Accession (Same CDS sequence)	XM_022410893.1
Accession Version	XM_022410893.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	F-actin-methionine sulfoxide oxidase MICAL3 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GGGCTCGATA AAGAAGGAGT TCCCACAGAA CTTAGGAGGC 
AGCGACACGT GCTATTTCTG CCGGAAGCGG GTCTACGTGA TGGAAAGGCT GAGTGCTGAG 
GGCAAGTTCT TCCATCGGAG CTGCTTCAAG TGCGAGTACT GCGCCACCAC CCTGCGCCTC 
TCGGCCTATG CCTATGACAT TGAGGACGGT AAATTCTACT GCAAACCACA CTACTGTTAT 
CGACTCTCTG GCTCTGCACA GAGGAAGAGA CCAGCAGTGG CTCCTCTCTC TGGAAAGGAG 
GCCAGAGAAC CCCTGCAGGA TGGCCCCACT GTGGACACAA ATGGACGGGC CAGCGCTGTC 
ACCAGCCCTG CTGAGAGAAC TCCAGGTTCA AGCGTGAATG GTGTGGAAGA GCCTAGCATT 
GCCAAGAGGC TCAGGGGCAC TCCCGAGCGG ATTGAGCTGG AGAACTACCG CCTGTCCGTG 
AAGCAGGCCG AGGAGCTGGA GGAGGTGCCC GAGGAGACAC AGGCCGAGCA CAACCTGAGC 
AGCGTGCTGG ACACGGGGGC AGAGGAGGAC GTGGCCAGCA GCAGTTCTGA GTCAGAGATG 
GAGGAAGAGG AGGAAGAAGA GCCTCCTCTG CCCACTTCGG ACCTGGGCGG TGTTCCTTGG 
AAGGAGGCCG TGAGGATCCA TGCTCTTCTG AAAGGAAAGA GTGAAGAGGA GCTAGAGGCC 
TCAAAGAGCT TTGGGCCTGG GCATGAAGAA GACGAGGAGG AGGAGGAGTA TGAAGAGGAG 
GAGGAGGAGG AGGAGGAAGA ATATGAAGAG GAAGAGGAAG AGGAAGAGTC TAGTGAAGAA 
GGGGAGTATT GCCCCTGGGA CAGAGAACTC CTACAAGGCC AGTGGCTCCG GCAGCTGTCA 
CAGGAAGAAA CTACGGGTAC ACGTAAAGTT GGGAGCCAGA GGCTACAGCA GATCGTAAAT 
CCGGCCGACC CCTTGGAGAT CCAGGCTGAT GTGCACTGGA CACATATCCG AGAGAGAGAG 
GAGGAGGAGC GAATGGTCCC AACCTCTGAG TCCTCCACTT CTAGAGTGCC TGACCTTCCC 
TCCATACGCC GCGCAGCAGT GCAGGCCTGG CTGGAGACGG TCTCTGGAGT CCCATTTGAT 
GAGAATGACC TGGAGGAAGA CGTGGACTCG GAGCCAGCAA AGATAGAAGG GGAGGCCACA 
GAGAATGGGG ACACAGGGGA CACTGGTGCT GAGCTGGACG ATGATCAGCG CTGGTCTGAC 
GGGATCCCAT CGGATGCCGA GACGGATCTC CGCGTGCAGC ACCAGGAGGC GGTAGAGGCA 
GACCTGGAGC TGAGGGTCTC AGAGAATGAG GAGGAGCCAC CTGAGGTCAC ACCAGGGCAT 
CCGGAGAGAA GTCTCACCCG GGTCCCCAGC CCTGCCCTGT CCCCTGAGGA GCAAGCTGGT 
CTGCGGTCCC CAGGCCACAG CCCTAAGGCA GAGGGGGCCC AAATCCCACT TGTCTCTGTT 
GTCACCAAGG CAAAATTGCC TGAAGAGAAC CTTTTCCCCA AGCCTTTGCT CCCCAAAGTG 
AAGCCCAAGG TGGAAGTGCC CTGCAGTCAG AGAGCTGCAT GCTCCCCCAT CCGTTCTCAG 
CCAGTGGCTC TGCCAGAAGC CAGGACACCT ACCTCCCCAG TGAGCTCGCA GTGCCTGTCG 
CCTTTGGCCA CCTCCACCCC CACCAGCACC CAGCTCCCCA TTTGCTCCCA GCCTCAGCCT 
TCCTCTGAGA CCACGGCCCC ATCCCCCACC AAGTCCCCCA TACGGCTCCA GCCTGTTTCA 
GCCAAAACAT CTATCCCAGT GGTTCCCCTC CCCTTGAAGA ACCAAGGGGA CACCAAGGAC 
AGGCTGGGCA GCCCTCTCGC TGTGGATGAG GCCCTGAAGC GGAATGACCT GGTGGCAGAG 
TTCTGGATGA AGAGCGCCGA GATCCGCCGC AGCCTCGGGC TCACACCAGT GGATCGGAGC 
AAGGTGTATG AGCCCAGCTT CCCTTCGCCT GCCTTTAAAC CAGTGTCCCT AAAATCCTAT 
GCAGTGGAGA AGTCTCCCGA GGGGGAGGGA CTCCAGCTTC TGAAGCCTCC ACCTGTTCCT 
AAGAGACTAG GCCTGCCGAA GTCAGACGGT GACCAGCCCT TCCTGCCAAC CCCCAAGTCC 
CCGTCGGACA GAGAGCTGAA AAGCTCCCAG GAGGAGAGGA GAGATCTGTC GAGCAGCTCC 
GGGCTGGGCC TTCACGGGAG CTCCTCCAAC ATGAAGACTT TGGGCAGCCA GAGCTTCAAC 
ACATCAGACT CCACCATGCT CACTCCCCCT TCAAGCCCAC CGCCACCACC ACCCCAAGAC 
GAGGAACCCG CAACTCTTCG GAGGAAGCCA TATCAGACTC ATGAGCATAA GGACGCTGAG 
CCCAAGGCCT CGGTGATTCC ACCACCACCA CCTGCGACGT TCATGCGGGC CCCCCGGGAG 
CCCACCCAGC CCCTCCAAGA GGAGATGCGG AAGTCGTTTG TGGAGAGCGT GGATGAGATC 
CCATTTGCCG ATGATGTGGA GGACACCTAT GATGACAAGA CCGAGGACTC GAGTCTCCAG 
GAGAAGTTCT TCACACCCCC CTCCTACTGG CCCCGCACGG AGAGGCCCCT CCACCCACCC 
CTAGCCAAGG AGAATGGTAG GTTGCCTACT CTGGAGAGTG GGGTCCAGCC ACAGAAGAGG 
GGCCTGCCCT TAGTCTCTCC AGAAGCCAAG GAGCTGGCTG AGGAGCGCAT GCGAGCCAGG 
GAGAAGTCTG TGAAGAGCCA GGCGTTGAGA GACGCTATGG CCAAGCAGCT GAGTAAGATG 
AAGGAAATGG AGATGGCGGC TGGGACCTCC AGTCCCCCAG GAGGCACCTC CCACAAAGCC 
TCCTCTGTTC CCTCCAAGGG CAAAGACCTC AGCCCCGAGT CCCCTAGACG CCCAGTTCTC 
AAAGGCCCCA GGGAGCCTGC CCTGAAGCAC GAAGCCACTA GTGAGGAGGT CCTCTCCCCA 
CCATCGGACT CAGGGGGCCC AGATGGTTCG GTCACCTCAT CCGAGGGCTC CAGTGGGAAG 
AGCAAGAAGA GATCGTCACT CTTCTCCCCC CGCAGAAACA AGAAAGAGAA GAAGTCCAAA 
GGTGAGGGCC GGCCCCCGGA GAAGCCCAGC CCAAGCCTTC TGGAGGAAGC TGCTGCCAAG 
CCCAAGTCCC TGTGGAAGTC GGTCTTTTCC GGGTACAAGA AGGACAAAAA GAAGAAGGGT 
GATGACAAGT CCTGTTCCAG CACCCCTTCC AGCGGTGCCA CTGTGGACTC TGGCAAGCAC 
AAGATGTCCC CTGTTGTGAG AGCAGAGCTG CACCTGCGGC GCCAGTTGAG CTTCTCCGAG 
GACTCAGACC TGTCCAGTGA TGACATCCTT GAGAGGTCCT CCCAGAAGTC CAGGCGAGAG 
TCGGTTTATG TACCACACGC CTTGGCATTC AAGAGATCAT ATCCATCAAA GCCGAGAACC 
TACACAGAGG AGGAACTGAA TGCCAAGCTG ACCCGGCGTG TGCAGAAGGC AGCTCGTAGA 
CAGGCCAAGC AAGAGGAGCT GAAGCGGCTG CACCGAGCAC AGATCATCCA GAGGCAGCTG 
GAGCAGGTGG AGGAGAAGCA GAGACAGCTG GAGGAAAGAG GGGTGGCCGT GGAGAAGGCG 
CTCCGTGGTG AAGCAGACTA TTGGGGAGAG TCTTATTACA GTGACCTCAT CGACTTGCAT 
CTGGGTGTTG AGCCCAGTGG CGGGACTCCA CGGCGTAGAC CTCTCTCCTT CTGCCCTTGC 
TGTGTCCAGG AAGGCATGGG CAAGAAGGAT GACCCCAAGC TGATGCAGGA GTGGTTCAAG 
TTGGTGCAAG AGAAGAATGC CATGGTGCGC TATGAGTCGG AGCTGATGAT TTTTGCCCGG 
GAGCTGGAAC TGGAGGACCG GCAGAGCCGA CTGCAGCAGG AGCTCCGGGA GCGGATGGCG 
GTGGAAGATC ACCTGAAGAC TGAGGAGGAG CTGTCTGAGG AGAAGAAGAT CTTGAACGAG 
ATGCTGGAGG TGGTGGAGCA GAGAGATGCC CTCGTGGCCC TGCTTGAGGA GCAGCGACTC 
CGGGAGAAGG AGGAGGACAA AGACCTGGAG GCTGTCATGC TGTCCAAGGG CTTCAGCCTG 
AACTGGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022266601.1
CDS	258..6629
Translation	MEDSKNETVNHAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDYRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKACTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFQGLVQPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELKEATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLNYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREQNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNININFLRPSQVRHLYNTGETRDIHLEMENLVNSRTTPKLTRNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGKEMASVGEPDKLSMVMYLTQFYEMFKDSLPSSDALDLNAEEKAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEEAPRGYRGGRPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSTGMRRQGSIKKEFPQNLGGSDTCYFCRKRVYVMERLSAEGKFFHRSCFKCEYCATTLRLSAYAYDIEDGKFYCKPHYCYRLSGSAQRKRPAVAPLSGKEAREPLQDGPTVDTNGRASAVTSPAERTPGSSVNGVEEPSIAKRLRGTPERIELENYRLSVKQAEELEEVPEETQAEHNLSSVLDTGAEEDVASSSSESEMEEEEEEEPPLPTSDLGGVPWKEAVRIHALLKGKSEEELEASKSFGPGHEEDEEEEEYEEEEEEEEEEYEEEEEEEESSEEGEYCPWDRELLQGQWLRQLSQEETTGTRKVGSQRLQQIVNPADPLEIQADVHWTHIREREEEERMVPTSESSTSRVPDLPSIRRAAVQAWLETVSGVPFDENDLEEDVDSEPAKIEGEATENGDTGDTGAELDDDQRWSDGIPSDAETDLRVQHQEAVEADLELRVSENEEEPPEVTPGHPERSLTRVPSPALSPEEQAGLRSPGHSPKAEGAQIPLVSVVTKAKLPEENLFPKPLLPKVKPKVEVPCSQRAACSPIRSQPVALPEARTPTSPVSSQCLSPLATSTPTSTQLPICSQPQPSSETTAPSPTKSPIRLQPVSAKTSIPVVPLPLKNQGDTKDRLGSPLAVDEALKRNDLVAEFWMKSAEIRRSLGLTPVDRSKVYEPSFPSPAFKPVSLKSYAVEKSPEGEGLQLLKPPPVPKRLGLPKSDGDQPFLPTPKSPSDRELKSSQEERRDLSSSSGLGLHGSSSNMKTLGSQSFNTSDSTMLTPPSSPPPPPPQDEEPATLRRKPYQTHEHKDAEPKASVIPPPPPATFMRAPREPTQPLQEEMRKSFVESVDEIPFADDVEDTYDDKTEDSSLQEKFFTPPSYWPRTERPLHPPLAKENGRLPTLESGVQPQKRGLPLVSPEAKELAEERMRAREKSVKSQALRDAMAKQLSKMKEMEMAAGTSSPPGGTSHKASSVPSKGKDLSPESPRRPVLKGPREPALKHEATSEEVLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRNKKEKKSKGEGRPPEKPSPSLLEEAAAKPKSLWKSVFSGYKKDKKKKGDDKSCSSTPSSGATVDSGKHKMSPVVRAELHLRRQLSFSEDSDLSSDDILERSSQKSRRESVYVPHALAFKRSYPSKPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELSEEKKILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAVMLSKGFSLNWS

Target ORF information:

RefSeq Version	XM_022410893.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022410893.1

ORF Insert Sequence:

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GGGCTCGATA AAGAAGGAGT TCCCACAGAA CTTAGGAGGC 
AGCGACACGT GCTATTTCTG CCGGAAGCGG GTCTACGTGA TGGAAAGGCT GAGTGCTGAG 
GGCAAGTTCT TCCATCGGAG CTGCTTCAAG TGCGAGTACT GCGCCACCAC CCTGCGCCTC 
TCGGCCTATG CCTATGACAT TGAGGACGGT AAATTCTACT GCAAACCACA CTACTGTTAT 
CGACTCTCTG GCTCTGCACA GAGGAAGAGA CCAGCAGTGG CTCCTCTCTC TGGAAAGGAG 
GCCAGAGAAC CCCTGCAGGA TGGCCCCACT GTGGACACAA ATGGACGGGC CAGCGCTGTC 
ACCAGCCCTG CTGAGAGAAC TCCAGGTTCA AGCGTGAATG GTGTGGAAGA GCCTAGCATT 
GCCAAGAGGC TCAGGGGCAC TCCCGAGCGG ATTGAGCTGG AGAACTACCG CCTGTCCGTG 
AAGCAGGCCG AGGAGCTGGA GGAGGTGCCC GAGGAGACAC AGGCCGAGCA CAACCTGAGC 
AGCGTGCTGG ACACGGGGGC AGAGGAGGAC GTGGCCAGCA GCAGTTCTGA GTCAGAGATG 
GAGGAAGAGG AGGAAGAAGA GCCTCCTCTG CCCACTTCGG ACCTGGGCGG TGTTCCTTGG 
AAGGAGGCCG TGAGGATCCA TGCTCTTCTG AAAGGAAAGA GTGAAGAGGA GCTAGAGGCC 
TCAAAGAGCT TTGGGCCTGG GCATGAAGAA GACGAGGAGG AGGAGGAGTA TGAAGAGGAG 
GAGGAGGAGG AGGAGGAAGA ATATGAAGAG GAAGAGGAAG AGGAAGAGTC TAGTGAAGAA 
GGGGAGTATT GCCCCTGGGA CAGAGAACTC CTACAAGGCC AGTGGCTCCG GCAGCTGTCA 
CAGGAAGAAA CTACGGGTAC ACGTAAAGTT GGGAGCCAGA GGCTACAGCA GATCGTAAAT 
CCGGCCGACC CCTTGGAGAT CCAGGCTGAT GTGCACTGGA CACATATCCG AGAGAGAGAG 
GAGGAGGAGC GAATGGTCCC AACCTCTGAG TCCTCCACTT CTAGAGTGCC TGACCTTCCC 
TCCATACGCC GCGCAGCAGT GCAGGCCTGG CTGGAGACGG TCTCTGGAGT CCCATTTGAT 
GAGAATGACC TGGAGGAAGA CGTGGACTCG GAGCCAGCAA AGATAGAAGG GGAGGCCACA 
GAGAATGGGG ACACAGGGGA CACTGGTGCT GAGCTGGACG ATGATCAGCG CTGGTCTGAC 
GGGATCCCAT CGGATGCCGA GACGGATCTC CGCGTGCAGC ACCAGGAGGC GGTAGAGGCA 
GACCTGGAGC TGAGGGTCTC AGAGAATGAG GAGGAGCCAC CTGAGGTCAC ACCAGGGCAT 
CCGGAGAGAA GTCTCACCCG GGTCCCCAGC CCTGCCCTGT CCCCTGAGGA GCAAGCTGGT 
CTGCGGTCCC CAGGCCACAG CCCTAAGGCA GAGGGGGCCC AAATCCCACT TGTCTCTGTT 
GTCACCAAGG CAAAATTGCC TGAAGAGAAC CTTTTCCCCA AGCCTTTGCT CCCCAAAGTG 
AAGCCCAAGG TGGAAGTGCC CTGCAGTCAG AGAGCTGCAT GCTCCCCCAT CCGTTCTCAG 
CCAGTGGCTC TGCCAGAAGC CAGGACACCT ACCTCCCCAG TGAGCTCGCA GTGCCTGTCG 
CCTTTGGCCA CCTCCACCCC CACCAGCACC CAGCTCCCCA TTTGCTCCCA GCCTCAGCCT 
TCCTCTGAGA CCACGGCCCC ATCCCCCACC AAGTCCCCCA TACGGCTCCA GCCTGTTTCA 
GCCAAAACAT CTATCCCAGT GGTTCCCCTC CCCTTGAAGA ACCAAGGGGA CACCAAGGAC 
AGGCTGGGCA GCCCTCTCGC TGTGGATGAG GCCCTGAAGC GGAATGACCT GGTGGCAGAG 
TTCTGGATGA AGAGCGCCGA GATCCGCCGC AGCCTCGGGC TCACACCAGT GGATCGGAGC 
AAGGTGTATG AGCCCAGCTT CCCTTCGCCT GCCTTTAAAC CAGTGTCCCT AAAATCCTAT 
GCAGTGGAGA AGTCTCCCGA GGGGGAGGGA CTCCAGCTTC TGAAGCCTCC ACCTGTTCCT 
AAGAGACTAG GCCTGCCGAA GTCAGACGGT GACCAGCCCT TCCTGCCAAC CCCCAAGTCC 
CCGTCGGACA GAGAGCTGAA AAGCTCCCAG GAGGAGAGGA GAGATCTGTC GAGCAGCTCC 
GGGCTGGGCC TTCACGGGAG CTCCTCCAAC ATGAAGACTT TGGGCAGCCA GAGCTTCAAC 
ACATCAGACT CCACCATGCT CACTCCCCCT TCAAGCCCAC CGCCACCACC ACCCCAAGAC 
GAGGAACCCG CAACTCTTCG GAGGAAGCCA TATCAGACTC ATGAGCATAA GGACGCTGAG 
CCCAAGGCCT CGGTGATTCC ACCACCACCA CCTGCGACGT TCATGCGGGC CCCCCGGGAG 
CCCACCCAGC CCCTCCAAGA GGAGATGCGG AAGTCGTTTG TGGAGAGCGT GGATGAGATC 
CCATTTGCCG ATGATGTGGA GGACACCTAT GATGACAAGA CCGAGGACTC GAGTCTCCAG 
GAGAAGTTCT TCACACCCCC CTCCTACTGG CCCCGCACGG AGAGGCCCCT CCACCCACCC 
CTAGCCAAGG AGAATGGTAG GTTGCCTACT CTGGAGAGTG GGGTCCAGCC ACAGAAGAGG 
GGCCTGCCCT TAGTCTCTCC AGAAGCCAAG GAGCTGGCTG AGGAGCGCAT GCGAGCCAGG 
GAGAAGTCTG TGAAGAGCCA GGCGTTGAGA GACGCTATGG CCAAGCAGCT GAGTAAGATG 
AAGGAAATGG AGATGGCGGC TGGGACCTCC AGTCCCCCAG GAGGCACCTC CCACAAAGCC 
TCCTCTGTTC CCTCCAAGGG CAAAGACCTC AGCCCCGAGT CCCCTAGACG CCCAGTTCTC 
AAAGGCCCCA GGGAGCCTGC CCTGAAGCAC GAAGCCACTA GTGAGGAGGT CCTCTCCCCA 
CCATCGGACT CAGGGGGCCC AGATGGTTCG GTCACCTCAT CCGAGGGCTC CAGTGGGAAG 
AGCAAGAAGA GATCGTCACT CTTCTCCCCC CGCAGAAACA AGAAAGAGAA GAAGTCCAAA 
GGTGAGGGCC GGCCCCCGGA GAAGCCCAGC CCAAGCCTTC TGGAGGAAGC TGCTGCCAAG 
CCCAAGTCCC TGTGGAAGTC GGTCTTTTCC GGGTACAAGA AGGACAAAAA GAAGAAGGGT 
GATGACAAGT CCTGTTCCAG CACCCCTTCC AGCGGTGCCA CTGTGGACTC TGGCAAGCAC 
AAGATGTCCC CTGTTGTGAG AGCAGAGCTG CACCTGCGGC GCCAGTTGAG CTTCTCCGAG 
GACTCAGACC TGTCCAGTGA TGACATCCTT GAGAGGTCCT CCCAGAAGTC CAGGCGAGAG 
TCGGTTTATG TACCACACGC CTTGGCATTC AAGAGATCAT ATCCATCAAA GCCGAGAACC 
TACACAGAGG AGGAACTGAA TGCCAAGCTG ACCCGGCGTG TGCAGAAGGC AGCTCGTAGA 
CAGGCCAAGC AAGAGGAGCT GAAGCGGCTG CACCGAGCAC AGATCATCCA GAGGCAGCTG 
GAGCAGGTGG AGGAGAAGCA GAGACAGCTG GAGGAAAGAG GGGTGGCCGT GGAGAAGGCG 
CTCCGTGGTG AAGCAGACTA TTGGGGAGAG TCTTATTACA GTGACCTCAT CGACTTGCAT 
CTGGGTGTTG AGCCCAGTGG CGGGACTCCA CGGCGTAGAC CTCTCTCCTT CTGCCCTTGC 
TGTGTCCAGG AAGGCATGGG CAAGAAGGAT GACCCCAAGC TGATGCAGGA GTGGTTCAAG 
TTGGTGCAAG AGAAGAATGC CATGGTGCGC TATGAGTCGG AGCTGATGAT TTTTGCCCGG 
GAGCTGGAAC TGGAGGACCG GCAGAGCCGA CTGCAGCAGG AGCTCCGGGA GCGGATGGCG 
GTGGAAGATC ACCTGAAGAC TGAGGAGGAG CTGTCTGAGG AGAAGAAGAT CTTGAACGAG 
ATGCTGGAGG TGGTGGAGCA GAGAGATGCC CTCGTGGCCC TGCTTGAGGA GCAGCGACTC 
CGGGAGAAGG AGGAGGACAA AGACCTGGAG GCTGTCATGC TGTCCAAGGG CTTCAGCCTG 
AACTGGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_022410896.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022266604.1 Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X27, mRNA.

Price & Availability↑

CloneID	OCe282690
Clone ID Related Accession (Same CDS sequence)	XM_022410896.1
Accession Version	XM_022410896.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3984bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	F-actin-methionine sulfoxide oxidase MICAL3 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTGCCTGAC CTTCCCTCCA TACGCCGCGC AGCAGTGCAG 
GCCTGGCTGG AGACGGTCTC TGGAGTCCCA TTTGATGAGA ATGACCTGGA GGAAGACGTG 
GACTCGGAGC CAGCAAAGAT AGAAGGGGAG GCCACAGAGA ATGGGGACAC AGGGGACACT 
GGTGCTGAGC TGGACGATGG GATGCCAGGG CCTCACTCGG GTGCTGTGCT CTCCCTTCGG 
CCCGCCAGAT CAGCGCTGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022266604.1
CDS	258..4241
Translation	MEDSKNETVNHAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDYRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKACTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFQGLVQPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELKEATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLNYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREQNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNININFLRPSQVRHLYNTGETRDIHLEMENLVNSRTTPKLTRNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGKEMASVGEPDKLSMVMYLTQFYEMFKDSLPSSDALDLNAEEKAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEEAPRGYRGGRPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSTGMRRQPTQERGVSQPSCCLPEQGHPAPTPQWKQQQEKERSRTCPKKVITLSPPLTPPPCRAPRSQQTYRDLDADSRGKQSPHHERPEPEPSRRFFVDQWELSLSLRSSNRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCRKRVYVMERLSAEGKFFHRSCFKCEYCATTLRLSAYAYDIEDGKFYCKPHYCYRLSGSAQRKRPAVAPLSGKEAREPLQDGPTVDTNGRASAVTSPAERTPGSSVNGVEEPSIAKRLRGTPERIELENYRLSVKQAEELEEVPEETQAEHNLSSVLDTGAEEDVASSSSESEMEEEEEEEPPLPTSDLGGVPWKEAVRIHALLKGKSEEELEASKSFGPGHEEDEEEEEYEEEEEEEEEEYEEEEEEEESSEEGEYCPWDRELLQGQWLRQLSQEETTGTRKVGSQRLQQIVNPADPLEIQADVHWTHIREREEEERMVPTSESSTSRVPDLPSIRRAAVQAWLETVSGVPFDENDLEEDVDSEPAKIEGEATENGDTGDTGAELDDGMPGPHSGAVLSLRPARSALV

Target ORF information:

RefSeq Version	XM_022410896.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022410896.1

ORF Insert Sequence:

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTGCCTGAC CTTCCCTCCA TACGCCGCGC AGCAGTGCAG 
GCCTGGCTGG AGACGGTCTC TGGAGTCCCA TTTGATGAGA ATGACCTGGA GGAAGACGTG 
GACTCGGAGC CAGCAAAGAT AGAAGGGGAG GCCACAGAGA ATGGGGACAC AGGGGACACT 
GGTGCTGAGC TGGACGATGG GATGCCAGGG CCTCACTCGG GTGCTGTGCT CTCCCTTCGG 
CCCGCCAGAT CAGCGCTGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_022410897.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022266605.1 Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X28, mRNA.

Price & Availability↑

CloneID	OCe282691
Clone ID Related Accession (Same CDS sequence)	XM_022410897.1
Accession Version	XM_022410897.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	F-actin-methionine sulfoxide oxidase MICAL3 isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTCCCATTT GATGAGAATG ACCTGGAGGA AGACGTGGAC 
TCGGAGCCAG CAAAGATAGA AGGGGAGGCC ACAGAGAATG GGGACACAGG GGACACTGGT 
GCTGAGCTGG ACGATGGGAT GCCAGGGCCT CACTCGGGTG CTGTGCTCTC CCTTCGGCCC 
GCCAGATCAG CGCTGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022266605.1
CDS	258..4178
Translation	MEDSKNETVNHAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDYRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKACTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFQGLVQPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELKEATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLNYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREQNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNININFLRPSQVRHLYNTGETRDIHLEMENLVNSRTTPKLTRNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGKEMASVGEPDKLSMVMYLTQFYEMFKDSLPSSDALDLNAEEKAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEEAPRGYRGGRPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSTGMRRQPTQERGVSQPSCCLPEQGHPAPTPQWKQQQEKERSRTCPKKVITLSPPLTPPPCRAPRSQQTYRDLDADSRGKQSPHHERPEPEPSRRFFVDQWELSLSLRSSNRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCRKRVYVMERLSAEGKFFHRSCFKCEYCATTLRLSAYAYDIEDGKFYCKPHYCYRLSGSAQRKRPAVAPLSGKEAREPLQDGPTVDTNGRASAVTSPAERTPGSSVNGVEEPSIAKRLRGTPERIELENYRLSVKQAEELEEVPEETQAEHNLSSVLDTGAEEDVASSSSESEMEEEEEEEPPLPTSDLGGVPWKEAVRIHALLKGKSEEELEASKSFGPGHEEDEEEEEYEEEEEEEEEEYEEEEEEEESSEEGEYCPWDRELLQGQWLRQLSQEETTGTRKVGSQRLQQIVNPADPLEIQADVHWTHIREREEEERMVPTSESSTSRVPFDENDLEEDVDSEPAKIEGEATENGDTGDTGAELDDGMPGPHSGAVLSLRPARSALV

Target ORF information:

RefSeq Version	XM_022410897.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022410897.1

ORF Insert Sequence:

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTCCCATTT GATGAGAATG ACCTGGAGGA AGACGTGGAC 
TCGGAGCCAG CAAAGATAGA AGGGGAGGCC ACAGAGAATG GGGACACAGG GGACACTGGT 
GCTGAGCTGG ACGATGGGAT GCCAGGGCCT CACTCGGGTG CTGTGCTCTC CCTTCGGCCC 
GCCAGATCAG CGCTGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_022410898.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022266606.1 Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X29, mRNA.

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CloneID	OCe282692
Clone ID Related Accession (Same CDS sequence)	XM_022410898.1
Accession Version	XM_022410898.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	F-actin-methionine sulfoxide oxidase MICAL3 isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAGTTAA TGTCCTGGAT CTGGAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022266606.1
CDS	258..3797
Translation	MEDSKNETVNHAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDYRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKACTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFQGLVQPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELKEATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLNYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREQNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNININFLRPSQVRHLYNTGETRDIHLEMENLVNSRTTPKLTRNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGKEMASVGEPDKLSMVMYLTQFYEMFKDSLPSSDALDLNAEEKAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEEAPRGYRGGRPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSTGMRRQPTQERGVSQPSCCLPEQGHPAPTPQWKQQQEKERSRTCPKKVITLSPPLTPPPCRAPRSQQTYRDLDADSRGKQSPHHERPEPEPSRRFFVDQWELSLSLRSSNRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCRKRVYVMERLSAEGKFFHRSCFKCEYCATTLRLSAYAYDIEDGKFYCKPHYCYRLSGSAQRKRPAVAPLSGKEAREPLQDGPTVDTNGRASAVTSPAERTPGSSVNGVEEPSIAKRLRGTPERIELENYRLSVKQAEELEEVPEETQAEHNLSSVLDTGAEEDVASSSSESEMEEEEEEEPPLPTSDLGGVPWKEAVRIHALLKGKSEEELEASKSFGPGHEEDEEEEEYEEEEEEEEEEYEEEEEEEESSEELMSWIWS

Target ORF information:

RefSeq Version	XM_022410898.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022410898.1

ORF Insert Sequence:

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAGTTAA TGTCCTGGAT CTGGAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_014108387.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963862.1 Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X5, mRNA.

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CloneID	OCe43613
Clone ID Related Accession (Same CDS sequence)	XM_014108387.2 , XM_014108388.2 , XM_014108383.1 , XM_014108384.1 , XM_014108383.2 , XM_022410874.1 , XM_014108386.1 , XM_014108386.2 , XM_022410875.1 , XM_014108385.1 , XM_014108387.1 , XM_014108388.1
Accession Version	XM_014108387.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	F-actin-methionine sulfoxide oxidase MICAL3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014108387.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTGCCTGAC CTTCCCTCCA TACGCCGCGC AGCAGTGCAG 
GCCTGGCTGG AGACGGTCTC TGGAGTCCCA TTTGATGAGA ATGACCTGGA GGAAGACGTG 
GACTCGGAGC CAGCAAAGAT AGAAGGGGAG GCCACAGAGA ATGGGGACAC AGGGGACACT 
GGTGCTGAGC TGGACGATGA TCAGCGCTGG TCTGACGGGA TCCCATCGGA TGCCGAGACG 
GATCTCCGCG TGCAGCACCA GGAGGCGGTA GAGGCAGACC TGGAGCTGAG GGTCTCAGAG 
AATGAGGAGG AGCCACCTGA GGTCACACCA GGGCATCCGG AGAGAAGTCT CACCCGGGTC 
CCCAGCCCTG CCCTGTCCCC TGAGGAGCAA GCTGGTCTGC GGTCCCCAGG CCACAGCCCT 
AAGGCAGAGG GGGCCCAAAT CCCACTTGTC TCTGTTGTCA CCAAGGCAAA ATTGCCTGAA 
GAGAACCTTT TCCCCAAGCC TTTGCTCCCC AAAGTGAAGC CCAAGGTGGA AGTGCCCTGC 
AGTCAGAGAG CTGCATGCTC CCCCATCCGT TCTCAGCCAG TGGCTCTGCC AGAAGCCAGG 
ACACCTACCT CCCCAGTGAG CTCGCAGTGC CTGTCGCCTT TGGCCACCTC CACCCCCACC 
AGCACCCAGC TCCCCATTTG CTCCCAGCCT CAGCCTTCCT CTGAGACCAC GGCCCCATCC 
CCCACCAAGT CCCCCATACG GCTCCAGCCT GTTTCAGCCA AAACATCTAT CCCAGTGGTT 
CCCCTCCCCT TGAAGAACCA AGGGGACACC AAGGACAGGC TGGGCAGCCC TCTCGCTGTG 
GATGAGGCCC TGAAGCGGAA TGACCTGGTG GCAGAGTTCT GGATGAAGAG CGCCGAGATC 
CGCCGCAGCC TCGGGCTCAC ACCAGTGGAT CGGAGCAAGG TGTATGAGCC CAGCTTCCCT 
TCGCCTGCCT TTAAACCAGT GTCCCTAAAA TCCTATGCAG TGGAGAAGTC TCCCGAGGGG 
GAGGGACTCC AGCTTCTGAA GCCTCCACCT GTTCCTAAGA GACTAGGCCT GCCGAAGTCA 
GACGGTGACC AGCCCTTCCT GCCAACCCCC AAGTCCCCGT CGGACAGAGA GCTGAAAAGC 
TCCCAGGAGG AGAGGAGAGA TCTGTCGAGC AGCTCCGGGC TGGGCCTTCA CGGGAGCTCC 
TCCAACATGA AGACTTTGGG CAGCCAGAGC TTCAACACAT CAGACTCCAC CATGCTCACT 
CCCCCTTCAA GCCCACCGCC ACCACCACCC CAAGACGAGG AACCCGCAAC TCTTCGGAGG 
AAGCCATATC AGACTCATGA GCATAAGGAC GCTGAGCCCA AGGCCTCGGT GATTCCACCA 
CCACCACCTG CGACGTTCAT GCGGGCCCCC CGGGAGCCCA CCCAGCCCCT CCAAGAGGAG 
ATGCGGAAGT CGTTTGTGGA GAGCGTGGAT GAGATCCCAT TTGCCGATGA TGTGGAGGAC 
ACCTATGATG ACAAGACCGA GGACTCGAGT CTCCAGGAGA AGTTCTTCAC ACCCCCCTCC 
TACTGGCCCC GCACGGAGAG GCCCCTCCAC CCACCCCTAG CCAAGGAGAA TGGTAGGTTG 
CCTACTCTGG AGAGTGGGGT CCAGCCACAG AAGAGGGGCC TGCCCTTAGT CTCTCCAGAA 
GCCAAGGAGC TGGCTGAGGA GCGCATGCGA GCCAGGGAGA AGTCTGTGAA GAGCCAGGCG 
TTGAGAGACG CTATGGCCAA GCAGCTGAGT AAGATGAAGG AAATGGAGAT GGCGGCTGGG 
ACCTCCAGTC CCCCAGGAGG CACCTCCCAC AAAGCCTCCT CTGTTCCCTC CAAGGGCAAA 
GACCTCAGCC CCGAGTCCCC TAGACGCCCA GTTCTCAAAG GCCCCAGGGA GCCTGCCCTG 
AAGCACGAAG CCACTAGTGA GGAGGTCCTC TCCCCACCAT CGGACTCAGG GGGCCCAGAT 
GGTTCGGTCA CCTCATCCGA GGGCTCCAGT GGGAAGAGCA AGAAGAGATC GTCACTCTTC 
TCCCCCCGCA GAAACAAGAA AGAGAAGAAG TCCAAAGGTG AGGGCCGGCC CCCGGAGAAG 
CCCAGCCCAA GCCTTCTGGA GGAAGCTGCT GCCAAGCCCA AGTCCCTGTG GAAGTCGGTC 
TTTTCCGGGT ACAAGAAGGA CAAAAAGAAG AAGGGTGATG ACAAGTCCTG TTCCAGCACC 
CCTTCCAGCG GTGCCACTGT GGACTCTGGC AAGCACAAGA TGTCCCCTGT TGTGAGAGCA 
GAGCTGCACC TGCGGCGCCA GTTGAGCTTC TCCGAGGACT CAGACCTGTC CAGTGATGAC 
ATCCTTGAGA GGTCCTCCCA GAAGTCCAGG CGAGAGTCGG TTTATGTACC ACACGCCTTG 
GCATTCAAGA GATCATATCC ATCAAAGCCG AGAACCTACA CAGAGGAGGA ACTGAATGCC 
AAGCTGACCC GGCGTGTGCA GAAGGCAGCT CGTAGACAGG CCAAGCAAGA GGAGCTGAAG 
CGGCTGCACC GAGCACAGAT CATCCAGAGG CAGCTGGAGC AGGTGGAGGA GAAGCAGAGA 
CAGCTGGAGG AAAGAGGGGT GGCCGTGGAG AAGGCGCTCC GTGGTGAAGC AGACTATTGG 
GGAGAGTCTT ATTACAGTGA CCTCATCGAC TTGCATCTGG GTGTTGAGCC CAGTGGCGGG 
ACTCCACGGC GTAGACCTCT CTCCTTCTGC CCTTGCTGTG TCCAGGAAGG CATGGGCAAG 
AAGGATGACC CCAAGCTGAT GCAGGAGTGG TTCAAGTTGG TGCAAGAGAA GAATGCCATG 
GTGCGCTATG AGTCGGAGCT GATGATTTTT GCCCGGGAGC TGGAACTGGA GGACCGGCAG 
AGCCGACTGC AGCAGGAGCT CCGGGAGCGG ATGGCGGTGG AAGATCACCT GAAGACTGAG 
GAGGAGCTGT CTGAGGAGAA GAAGATCTTG AACGAGATGC TGGAGGTGGT GGAGCAGAGA 
GATGCCCTCG TGGCCCTGCT TGAGGAGCAG CGACTCCGGG AGAAGGAGGA GGACAAAGAC 
CTGGAGGCTG TCATGCTGTC CAAGGGCTTC AGCCTGAACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963862.1
CDS	253..7080
Translation	MEDSKNETVNHAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDYRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKACTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFQGLVQPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELKEATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLNYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREQNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNININFLRPSQVRHLYNTGETRDIHLEMENLVNSRTTPKLTRNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGKEMASVGEPDKLSMVMYLTQFYEMFKDSLPSSDALDLNAEEKAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEEAPRGYRGGRPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSTGMRRQPTQERGVSQPSCCLPEQGHPAPTPQWKQQQEKERSRTCPKKVITLSPPLTPPPCRAPRSQQTYRDLDADSRGKQSPHHERPEPEPSRRFFVDQWELSLSLRSSNRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCRKRVYVMERLSAEGKFFHRSCFKCEYCATTLRLSAYAYDIEDGKFYCKPHYCYRLSGSAQRKRPAVAPLSGKEAREPLQDGPTVDTNGRASAVTSPAERTPGSSVNGVEEPSIAKRLRGTPERIELENYRLSVKQAEELEEVPEETQAEHNLSSVLDTGAEEDVASSSSESEMEEEEEEEPPLPTSDLGGVPWKEAVRIHALLKGKSEEELEASKSFGPGHEEDEEEEEYEEEEEEEEEEYEEEEEEEESSEEGEYCPWDRELLQGQWLRQLSQEETTGTRKVGSQRLQQIVNPADPLEIQADVHWTHIREREEEERMVPTSESSTSRVPDLPSIRRAAVQAWLETVSGVPFDENDLEEDVDSEPAKIEGEATENGDTGDTGAELDDDQRWSDGIPSDAETDLRVQHQEAVEADLELRVSENEEEPPEVTPGHPERSLTRVPSPALSPEEQAGLRSPGHSPKAEGAQIPLVSVVTKAKLPEENLFPKPLLPKVKPKVEVPCSQRAACSPIRSQPVALPEARTPTSPVSSQCLSPLATSTPTSTQLPICSQPQPSSETTAPSPTKSPIRLQPVSAKTSIPVVPLPLKNQGDTKDRLGSPLAVDEALKRNDLVAEFWMKSAEIRRSLGLTPVDRSKVYEPSFPSPAFKPVSLKSYAVEKSPEGEGLQLLKPPPVPKRLGLPKSDGDQPFLPTPKSPSDRELKSSQEERRDLSSSSGLGLHGSSSNMKTLGSQSFNTSDSTMLTPPSSPPPPPPQDEEPATLRRKPYQTHEHKDAEPKASVIPPPPPATFMRAPREPTQPLQEEMRKSFVESVDEIPFADDVEDTYDDKTEDSSLQEKFFTPPSYWPRTERPLHPPLAKENGRLPTLESGVQPQKRGLPLVSPEAKELAEERMRAREKSVKSQALRDAMAKQLSKMKEMEMAAGTSSPPGGTSHKASSVPSKGKDLSPESPRRPVLKGPREPALKHEATSEEVLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRNKKEKKSKGEGRPPEKPSPSLLEEAAAKPKSLWKSVFSGYKKDKKKKGDDKSCSSTPSSGATVDSGKHKMSPVVRAELHLRRQLSFSEDSDLSSDDILERSSQKSRRESVYVPHALAFKRSYPSKPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELSEEKKILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAVMLSKGFSLNWS

Target ORF information:

RefSeq Version	XM_014108387.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108387.2

ORF Insert Sequence:

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTGCCTGAC CTTCCCTCCA TACGCCGCGC AGCAGTGCAG 
GCCTGGCTGG AGACGGTCTC TGGAGTCCCA TTTGATGAGA ATGACCTGGA GGAAGACGTG 
GACTCGGAGC CAGCAAAGAT AGAAGGGGAG GCCACAGAGA ATGGGGACAC AGGGGACACT 
GGTGCTGAGC TGGACGATGA TCAGCGCTGG TCTGACGGGA TCCCATCGGA TGCCGAGACG 
GATCTCCGCG TGCAGCACCA GGAGGCGGTA GAGGCAGACC TGGAGCTGAG GGTCTCAGAG 
AATGAGGAGG AGCCACCTGA GGTCACACCA GGGCATCCGG AGAGAAGTCT CACCCGGGTC 
CCCAGCCCTG CCCTGTCCCC TGAGGAGCAA GCTGGTCTGC GGTCCCCAGG CCACAGCCCT 
AAGGCAGAGG GGGCCCAAAT CCCACTTGTC TCTGTTGTCA CCAAGGCAAA ATTGCCTGAA 
GAGAACCTTT TCCCCAAGCC TTTGCTCCCC AAAGTGAAGC CCAAGGTGGA AGTGCCCTGC 
AGTCAGAGAG CTGCATGCTC CCCCATCCGT TCTCAGCCAG TGGCTCTGCC AGAAGCCAGG 
ACACCTACCT CCCCAGTGAG CTCGCAGTGC CTGTCGCCTT TGGCCACCTC CACCCCCACC 
AGCACCCAGC TCCCCATTTG CTCCCAGCCT CAGCCTTCCT CTGAGACCAC GGCCCCATCC 
CCCACCAAGT CCCCCATACG GCTCCAGCCT GTTTCAGCCA AAACATCTAT CCCAGTGGTT 
CCCCTCCCCT TGAAGAACCA AGGGGACACC AAGGACAGGC TGGGCAGCCC TCTCGCTGTG 
GATGAGGCCC TGAAGCGGAA TGACCTGGTG GCAGAGTTCT GGATGAAGAG CGCCGAGATC 
CGCCGCAGCC TCGGGCTCAC ACCAGTGGAT CGGAGCAAGG TGTATGAGCC CAGCTTCCCT 
TCGCCTGCCT TTAAACCAGT GTCCCTAAAA TCCTATGCAG TGGAGAAGTC TCCCGAGGGG 
GAGGGACTCC AGCTTCTGAA GCCTCCACCT GTTCCTAAGA GACTAGGCCT GCCGAAGTCA 
GACGGTGACC AGCCCTTCCT GCCAACCCCC AAGTCCCCGT CGGACAGAGA GCTGAAAAGC 
TCCCAGGAGG AGAGGAGAGA TCTGTCGAGC AGCTCCGGGC TGGGCCTTCA CGGGAGCTCC 
TCCAACATGA AGACTTTGGG CAGCCAGAGC TTCAACACAT CAGACTCCAC CATGCTCACT 
CCCCCTTCAA GCCCACCGCC ACCACCACCC CAAGACGAGG AACCCGCAAC TCTTCGGAGG 
AAGCCATATC AGACTCATGA GCATAAGGAC GCTGAGCCCA AGGCCTCGGT GATTCCACCA 
CCACCACCTG CGACGTTCAT GCGGGCCCCC CGGGAGCCCA CCCAGCCCCT CCAAGAGGAG 
ATGCGGAAGT CGTTTGTGGA GAGCGTGGAT GAGATCCCAT TTGCCGATGA TGTGGAGGAC 
ACCTATGATG ACAAGACCGA GGACTCGAGT CTCCAGGAGA AGTTCTTCAC ACCCCCCTCC 
TACTGGCCCC GCACGGAGAG GCCCCTCCAC CCACCCCTAG CCAAGGAGAA TGGTAGGTTG 
CCTACTCTGG AGAGTGGGGT CCAGCCACAG AAGAGGGGCC TGCCCTTAGT CTCTCCAGAA 
GCCAAGGAGC TGGCTGAGGA GCGCATGCGA GCCAGGGAGA AGTCTGTGAA GAGCCAGGCG 
TTGAGAGACG CTATGGCCAA GCAGCTGAGT AAGATGAAGG AAATGGAGAT GGCGGCTGGG 
ACCTCCAGTC CCCCAGGAGG CACCTCCCAC AAAGCCTCCT CTGTTCCCTC CAAGGGCAAA 
GACCTCAGCC CCGAGTCCCC TAGACGCCCA GTTCTCAAAG GCCCCAGGGA GCCTGCCCTG 
AAGCACGAAG CCACTAGTGA GGAGGTCCTC TCCCCACCAT CGGACTCAGG GGGCCCAGAT 
GGTTCGGTCA CCTCATCCGA GGGCTCCAGT GGGAAGAGCA AGAAGAGATC GTCACTCTTC 
TCCCCCCGCA GAAACAAGAA AGAGAAGAAG TCCAAAGGTG AGGGCCGGCC CCCGGAGAAG 
CCCAGCCCAA GCCTTCTGGA GGAAGCTGCT GCCAAGCCCA AGTCCCTGTG GAAGTCGGTC 
TTTTCCGGGT ACAAGAAGGA CAAAAAGAAG AAGGGTGATG ACAAGTCCTG TTCCAGCACC 
CCTTCCAGCG GTGCCACTGT GGACTCTGGC AAGCACAAGA TGTCCCCTGT TGTGAGAGCA 
GAGCTGCACC TGCGGCGCCA GTTGAGCTTC TCCGAGGACT CAGACCTGTC CAGTGATGAC 
ATCCTTGAGA GGTCCTCCCA GAAGTCCAGG CGAGAGTCGG TTTATGTACC ACACGCCTTG 
GCATTCAAGA GATCATATCC ATCAAAGCCG AGAACCTACA CAGAGGAGGA ACTGAATGCC 
AAGCTGACCC GGCGTGTGCA GAAGGCAGCT CGTAGACAGG CCAAGCAAGA GGAGCTGAAG 
CGGCTGCACC GAGCACAGAT CATCCAGAGG CAGCTGGAGC AGGTGGAGGA GAAGCAGAGA 
CAGCTGGAGG AAAGAGGGGT GGCCGTGGAG AAGGCGCTCC GTGGTGAAGC AGACTATTGG 
GGAGAGTCTT ATTACAGTGA CCTCATCGAC TTGCATCTGG GTGTTGAGCC CAGTGGCGGG 
ACTCCACGGC GTAGACCTCT CTCCTTCTGC CCTTGCTGTG TCCAGGAAGG CATGGGCAAG 
AAGGATGACC CCAAGCTGAT GCAGGAGTGG TTCAAGTTGG TGCAAGAGAA GAATGCCATG 
GTGCGCTATG AGTCGGAGCT GATGATTTTT GCCCGGGAGC TGGAACTGGA GGACCGGCAG 
AGCCGACTGC AGCAGGAGCT CCGGGAGCGG ATGGCGGTGG AAGATCACCT GAAGACTGAG 
GAGGAGCTGT CTGAGGAGAA GAAGATCTTG AACGAGATGC TGGAGGTGGT GGAGCAGAGA 
GATGCCCTCG TGGCCCTGCT TGAGGAGCAG CGACTCCGGG AGAAGGAGGA GGACAAAGAC 
CTGGAGGCTG TCATGCTGTC CAAGGGCTTC AGCCTGAACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_014108388.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963863.1 Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X6, mRNA.

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CloneID	OCe43613
Clone ID Related Accession (Same CDS sequence)	XM_014108387.2 , XM_014108388.2 , XM_014108383.1 , XM_014108384.1 , XM_014108383.2 , XM_022410874.1 , XM_014108386.1 , XM_014108386.2 , XM_022410875.1 , XM_014108385.1 , XM_014108387.1 , XM_014108388.1
Accession Version	XM_014108388.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	F-actin-methionine sulfoxide oxidase MICAL3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014108388.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTGCCTGAC CTTCCCTCCA TACGCCGCGC AGCAGTGCAG 
GCCTGGCTGG AGACGGTCTC TGGAGTCCCA TTTGATGAGA ATGACCTGGA GGAAGACGTG 
GACTCGGAGC CAGCAAAGAT AGAAGGGGAG GCCACAGAGA ATGGGGACAC AGGGGACACT 
GGTGCTGAGC TGGACGATGA TCAGCGCTGG TCTGACGGGA TCCCATCGGA TGCCGAGACG 
GATCTCCGCG TGCAGCACCA GGAGGCGGTA GAGGCAGACC TGGAGCTGAG GGTCTCAGAG 
AATGAGGAGG AGCCACCTGA GGTCACACCA GGGCATCCGG AGAGAAGTCT CACCCGGGTC 
CCCAGCCCTG CCCTGTCCCC TGAGGAGCAA GCTGGTCTGC GGTCCCCAGG CCACAGCCCT 
AAGGCAGAGG GGGCCCAAAT CCCACTTGTC TCTGTTGTCA CCAAGGCAAA ATTGCCTGAA 
GAGAACCTTT TCCCCAAGCC TTTGCTCCCC AAAGTGAAGC CCAAGGTGGA AGTGCCCTGC 
AGTCAGAGAG CTGCATGCTC CCCCATCCGT TCTCAGCCAG TGGCTCTGCC AGAAGCCAGG 
ACACCTACCT CCCCAGTGAG CTCGCAGTGC CTGTCGCCTT TGGCCACCTC CACCCCCACC 
AGCACCCAGC TCCCCATTTG CTCCCAGCCT CAGCCTTCCT CTGAGACCAC GGCCCCATCC 
CCCACCAAGT CCCCCATACG GCTCCAGCCT GTTTCAGCCA AAACATCTAT CCCAGTGGTT 
CCCCTCCCCT TGAAGAACCA AGGGGACACC AAGGACAGGC TGGGCAGCCC TCTCGCTGTG 
GATGAGGCCC TGAAGCGGAA TGACCTGGTG GCAGAGTTCT GGATGAAGAG CGCCGAGATC 
CGCCGCAGCC TCGGGCTCAC ACCAGTGGAT CGGAGCAAGG TGTATGAGCC CAGCTTCCCT 
TCGCCTGCCT TTAAACCAGT GTCCCTAAAA TCCTATGCAG TGGAGAAGTC TCCCGAGGGG 
GAGGGACTCC AGCTTCTGAA GCCTCCACCT GTTCCTAAGA GACTAGGCCT GCCGAAGTCA 
GACGGTGACC AGCCCTTCCT GCCAACCCCC AAGTCCCCGT CGGACAGAGA GCTGAAAAGC 
TCCCAGGAGG AGAGGAGAGA TCTGTCGAGC AGCTCCGGGC TGGGCCTTCA CGGGAGCTCC 
TCCAACATGA AGACTTTGGG CAGCCAGAGC TTCAACACAT CAGACTCCAC CATGCTCACT 
CCCCCTTCAA GCCCACCGCC ACCACCACCC CAAGACGAGG AACCCGCAAC TCTTCGGAGG 
AAGCCATATC AGACTCATGA GCATAAGGAC GCTGAGCCCA AGGCCTCGGT GATTCCACCA 
CCACCACCTG CGACGTTCAT GCGGGCCCCC CGGGAGCCCA CCCAGCCCCT CCAAGAGGAG 
ATGCGGAAGT CGTTTGTGGA GAGCGTGGAT GAGATCCCAT TTGCCGATGA TGTGGAGGAC 
ACCTATGATG ACAAGACCGA GGACTCGAGT CTCCAGGAGA AGTTCTTCAC ACCCCCCTCC 
TACTGGCCCC GCACGGAGAG GCCCCTCCAC CCACCCCTAG CCAAGGAGAA TGGTAGGTTG 
CCTACTCTGG AGAGTGGGGT CCAGCCACAG AAGAGGGGCC TGCCCTTAGT CTCTCCAGAA 
GCCAAGGAGC TGGCTGAGGA GCGCATGCGA GCCAGGGAGA AGTCTGTGAA GAGCCAGGCG 
TTGAGAGACG CTATGGCCAA GCAGCTGAGT AAGATGAAGG AAATGGAGAT GGCGGCTGGG 
ACCTCCAGTC CCCCAGGAGG CACCTCCCAC AAAGCCTCCT CTGTTCCCTC CAAGGGCAAA 
GACCTCAGCC CCGAGTCCCC TAGACGCCCA GTTCTCAAAG GCCCCAGGGA GCCTGCCCTG 
AAGCACGAAG CCACTAGTGA GGAGGTCCTC TCCCCACCAT CGGACTCAGG GGGCCCAGAT 
GGTTCGGTCA CCTCATCCGA GGGCTCCAGT GGGAAGAGCA AGAAGAGATC GTCACTCTTC 
TCCCCCCGCA GAAACAAGAA AGAGAAGAAG TCCAAAGGTG AGGGCCGGCC CCCGGAGAAG 
CCCAGCCCAA GCCTTCTGGA GGAAGCTGCT GCCAAGCCCA AGTCCCTGTG GAAGTCGGTC 
TTTTCCGGGT ACAAGAAGGA CAAAAAGAAG AAGGGTGATG ACAAGTCCTG TTCCAGCACC 
CCTTCCAGCG GTGCCACTGT GGACTCTGGC AAGCACAAGA TGTCCCCTGT TGTGAGAGCA 
GAGCTGCACC TGCGGCGCCA GTTGAGCTTC TCCGAGGACT CAGACCTGTC CAGTGATGAC 
ATCCTTGAGA GGTCCTCCCA GAAGTCCAGG CGAGAGTCGG TTTATGTACC ACACGCCTTG 
GCATTCAAGA GATCATATCC ATCAAAGCCG AGAACCTACA CAGAGGAGGA ACTGAATGCC 
AAGCTGACCC GGCGTGTGCA GAAGGCAGCT CGTAGACAGG CCAAGCAAGA GGAGCTGAAG 
CGGCTGCACC GAGCACAGAT CATCCAGAGG CAGCTGGAGC AGGTGGAGGA GAAGCAGAGA 
CAGCTGGAGG AAAGAGGGGT GGCCGTGGAG AAGGCGCTCC GTGGTGAAGC AGACTATTGG 
GGAGAGTCTT ATTACAGTGA CCTCATCGAC TTGCATCTGG GTGTTGAGCC CAGTGGCGGG 
ACTCCACGGC GTAGACCTCT CTCCTTCTGC CCTTGCTGTG TCCAGGAAGG CATGGGCAAG 
AAGGATGACC CCAAGCTGAT GCAGGAGTGG TTCAAGTTGG TGCAAGAGAA GAATGCCATG 
GTGCGCTATG AGTCGGAGCT GATGATTTTT GCCCGGGAGC TGGAACTGGA GGACCGGCAG 
AGCCGACTGC AGCAGGAGCT CCGGGAGCGG ATGGCGGTGG AAGATCACCT GAAGACTGAG 
GAGGAGCTGT CTGAGGAGAA GAAGATCTTG AACGAGATGC TGGAGGTGGT GGAGCAGAGA 
GATGCCCTCG TGGCCCTGCT TGAGGAGCAG CGACTCCGGG AGAAGGAGGA GGACAAAGAC 
CTGGAGGCTG TCATGCTGTC CAAGGGCTTC AGCCTGAACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963863.1
CDS	176..7003
Translation	MEDSKNETVNHAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDYRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKACTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFQGLVQPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELKEATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLNYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREQNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNININFLRPSQVRHLYNTGETRDIHLEMENLVNSRTTPKLTRNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGKEMASVGEPDKLSMVMYLTQFYEMFKDSLPSSDALDLNAEEKAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEEAPRGYRGGRPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSTGMRRQPTQERGVSQPSCCLPEQGHPAPTPQWKQQQEKERSRTCPKKVITLSPPLTPPPCRAPRSQQTYRDLDADSRGKQSPHHERPEPEPSRRFFVDQWELSLSLRSSNRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCRKRVYVMERLSAEGKFFHRSCFKCEYCATTLRLSAYAYDIEDGKFYCKPHYCYRLSGSAQRKRPAVAPLSGKEAREPLQDGPTVDTNGRASAVTSPAERTPGSSVNGVEEPSIAKRLRGTPERIELENYRLSVKQAEELEEVPEETQAEHNLSSVLDTGAEEDVASSSSESEMEEEEEEEPPLPTSDLGGVPWKEAVRIHALLKGKSEEELEASKSFGPGHEEDEEEEEYEEEEEEEEEEYEEEEEEEESSEEGEYCPWDRELLQGQWLRQLSQEETTGTRKVGSQRLQQIVNPADPLEIQADVHWTHIREREEEERMVPTSESSTSRVPDLPSIRRAAVQAWLETVSGVPFDENDLEEDVDSEPAKIEGEATENGDTGDTGAELDDDQRWSDGIPSDAETDLRVQHQEAVEADLELRVSENEEEPPEVTPGHPERSLTRVPSPALSPEEQAGLRSPGHSPKAEGAQIPLVSVVTKAKLPEENLFPKPLLPKVKPKVEVPCSQRAACSPIRSQPVALPEARTPTSPVSSQCLSPLATSTPTSTQLPICSQPQPSSETTAPSPTKSPIRLQPVSAKTSIPVVPLPLKNQGDTKDRLGSPLAVDEALKRNDLVAEFWMKSAEIRRSLGLTPVDRSKVYEPSFPSPAFKPVSLKSYAVEKSPEGEGLQLLKPPPVPKRLGLPKSDGDQPFLPTPKSPSDRELKSSQEERRDLSSSSGLGLHGSSSNMKTLGSQSFNTSDSTMLTPPSSPPPPPPQDEEPATLRRKPYQTHEHKDAEPKASVIPPPPPATFMRAPREPTQPLQEEMRKSFVESVDEIPFADDVEDTYDDKTEDSSLQEKFFTPPSYWPRTERPLHPPLAKENGRLPTLESGVQPQKRGLPLVSPEAKELAEERMRAREKSVKSQALRDAMAKQLSKMKEMEMAAGTSSPPGGTSHKASSVPSKGKDLSPESPRRPVLKGPREPALKHEATSEEVLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRNKKEKKSKGEGRPPEKPSPSLLEEAAAKPKSLWKSVFSGYKKDKKKKGDDKSCSSTPSSGATVDSGKHKMSPVVRAELHLRRQLSFSEDSDLSSDDILERSSQKSRRESVYVPHALAFKRSYPSKPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELSEEKKILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAVMLSKGFSLNWS

Target ORF information:

RefSeq Version	XM_014108388.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108388.2

ORF Insert Sequence:

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTGCCTGAC CTTCCCTCCA TACGCCGCGC AGCAGTGCAG 
GCCTGGCTGG AGACGGTCTC TGGAGTCCCA TTTGATGAGA ATGACCTGGA GGAAGACGTG 
GACTCGGAGC CAGCAAAGAT AGAAGGGGAG GCCACAGAGA ATGGGGACAC AGGGGACACT 
GGTGCTGAGC TGGACGATGA TCAGCGCTGG TCTGACGGGA TCCCATCGGA TGCCGAGACG 
GATCTCCGCG TGCAGCACCA GGAGGCGGTA GAGGCAGACC TGGAGCTGAG GGTCTCAGAG 
AATGAGGAGG AGCCACCTGA GGTCACACCA GGGCATCCGG AGAGAAGTCT CACCCGGGTC 
CCCAGCCCTG CCCTGTCCCC TGAGGAGCAA GCTGGTCTGC GGTCCCCAGG CCACAGCCCT 
AAGGCAGAGG GGGCCCAAAT CCCACTTGTC TCTGTTGTCA CCAAGGCAAA ATTGCCTGAA 
GAGAACCTTT TCCCCAAGCC TTTGCTCCCC AAAGTGAAGC CCAAGGTGGA AGTGCCCTGC 
AGTCAGAGAG CTGCATGCTC CCCCATCCGT TCTCAGCCAG TGGCTCTGCC AGAAGCCAGG 
ACACCTACCT CCCCAGTGAG CTCGCAGTGC CTGTCGCCTT TGGCCACCTC CACCCCCACC 
AGCACCCAGC TCCCCATTTG CTCCCAGCCT CAGCCTTCCT CTGAGACCAC GGCCCCATCC 
CCCACCAAGT CCCCCATACG GCTCCAGCCT GTTTCAGCCA AAACATCTAT CCCAGTGGTT 
CCCCTCCCCT TGAAGAACCA AGGGGACACC AAGGACAGGC TGGGCAGCCC TCTCGCTGTG 
GATGAGGCCC TGAAGCGGAA TGACCTGGTG GCAGAGTTCT GGATGAAGAG CGCCGAGATC 
CGCCGCAGCC TCGGGCTCAC ACCAGTGGAT CGGAGCAAGG TGTATGAGCC CAGCTTCCCT 
TCGCCTGCCT TTAAACCAGT GTCCCTAAAA TCCTATGCAG TGGAGAAGTC TCCCGAGGGG 
GAGGGACTCC AGCTTCTGAA GCCTCCACCT GTTCCTAAGA GACTAGGCCT GCCGAAGTCA 
GACGGTGACC AGCCCTTCCT GCCAACCCCC AAGTCCCCGT CGGACAGAGA GCTGAAAAGC 
TCCCAGGAGG AGAGGAGAGA TCTGTCGAGC AGCTCCGGGC TGGGCCTTCA CGGGAGCTCC 
TCCAACATGA AGACTTTGGG CAGCCAGAGC TTCAACACAT CAGACTCCAC CATGCTCACT 
CCCCCTTCAA GCCCACCGCC ACCACCACCC CAAGACGAGG AACCCGCAAC TCTTCGGAGG 
AAGCCATATC AGACTCATGA GCATAAGGAC GCTGAGCCCA AGGCCTCGGT GATTCCACCA 
CCACCACCTG CGACGTTCAT GCGGGCCCCC CGGGAGCCCA CCCAGCCCCT CCAAGAGGAG 
ATGCGGAAGT CGTTTGTGGA GAGCGTGGAT GAGATCCCAT TTGCCGATGA TGTGGAGGAC 
ACCTATGATG ACAAGACCGA GGACTCGAGT CTCCAGGAGA AGTTCTTCAC ACCCCCCTCC 
TACTGGCCCC GCACGGAGAG GCCCCTCCAC CCACCCCTAG CCAAGGAGAA TGGTAGGTTG 
CCTACTCTGG AGAGTGGGGT CCAGCCACAG AAGAGGGGCC TGCCCTTAGT CTCTCCAGAA 
GCCAAGGAGC TGGCTGAGGA GCGCATGCGA GCCAGGGAGA AGTCTGTGAA GAGCCAGGCG 
TTGAGAGACG CTATGGCCAA GCAGCTGAGT AAGATGAAGG AAATGGAGAT GGCGGCTGGG 
ACCTCCAGTC CCCCAGGAGG CACCTCCCAC AAAGCCTCCT CTGTTCCCTC CAAGGGCAAA 
GACCTCAGCC CCGAGTCCCC TAGACGCCCA GTTCTCAAAG GCCCCAGGGA GCCTGCCCTG 
AAGCACGAAG CCACTAGTGA GGAGGTCCTC TCCCCACCAT CGGACTCAGG GGGCCCAGAT 
GGTTCGGTCA CCTCATCCGA GGGCTCCAGT GGGAAGAGCA AGAAGAGATC GTCACTCTTC 
TCCCCCCGCA GAAACAAGAA AGAGAAGAAG TCCAAAGGTG AGGGCCGGCC CCCGGAGAAG 
CCCAGCCCAA GCCTTCTGGA GGAAGCTGCT GCCAAGCCCA AGTCCCTGTG GAAGTCGGTC 
TTTTCCGGGT ACAAGAAGGA CAAAAAGAAG AAGGGTGATG ACAAGTCCTG TTCCAGCACC 
CCTTCCAGCG GTGCCACTGT GGACTCTGGC AAGCACAAGA TGTCCCCTGT TGTGAGAGCA 
GAGCTGCACC TGCGGCGCCA GTTGAGCTTC TCCGAGGACT CAGACCTGTC CAGTGATGAC 
ATCCTTGAGA GGTCCTCCCA GAAGTCCAGG CGAGAGTCGG TTTATGTACC ACACGCCTTG 
GCATTCAAGA GATCATATCC ATCAAAGCCG AGAACCTACA CAGAGGAGGA ACTGAATGCC 
AAGCTGACCC GGCGTGTGCA GAAGGCAGCT CGTAGACAGG CCAAGCAAGA GGAGCTGAAG 
CGGCTGCACC GAGCACAGAT CATCCAGAGG CAGCTGGAGC AGGTGGAGGA GAAGCAGAGA 
CAGCTGGAGG AAAGAGGGGT GGCCGTGGAG AAGGCGCTCC GTGGTGAAGC AGACTATTGG 
GGAGAGTCTT ATTACAGTGA CCTCATCGAC TTGCATCTGG GTGTTGAGCC CAGTGGCGGG 
ACTCCACGGC GTAGACCTCT CTCCTTCTGC CCTTGCTGTG TCCAGGAAGG CATGGGCAAG 
AAGGATGACC CCAAGCTGAT GCAGGAGTGG TTCAAGTTGG TGCAAGAGAA GAATGCCATG 
GTGCGCTATG AGTCGGAGCT GATGATTTTT GCCCGGGAGC TGGAACTGGA GGACCGGCAG 
AGCCGACTGC AGCAGGAGCT CCGGGAGCGG ATGGCGGTGG AAGATCACCT GAAGACTGAG 
GAGGAGCTGT CTGAGGAGAA GAAGATCTTG AACGAGATGC TGGAGGTGGT GGAGCAGAGA 
GATGCCCTCG TGGCCCTGCT TGAGGAGCAG CGACTCCGGG AGAAGGAGGA GGACAAAGAC 
CTGGAGGCTG TCATGCTGTC CAAGGGCTTC AGCCTGAACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_014108383.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963858.1 Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X1, mRNA.

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CloneID	OCe43613
Clone ID Related Accession (Same CDS sequence)	XM_014108387.2 , XM_014108388.2 , XM_014108383.1 , XM_014108384.1 , XM_014108383.2 , XM_022410874.1 , XM_014108386.1 , XM_014108386.2 , XM_022410875.1 , XM_014108385.1 , XM_014108387.1 , XM_014108388.1
Accession Version	XM_014108383.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTGCCTGAC CTTCCCTCCA TACGCCGCGC AGCAGTGCAG 
GCCTGGCTGG AGACGGTCTC TGGAGTCCCA TTTGATGAGA ATGACCTGGA GGAAGACGTG 
GACTCGGAGC CAGCAAAGAT AGAAGGGGAG GCCACAGAGA ATGGGGACAC AGGGGACACT 
GGTGCTGAGC TGGACGATGA TCAGCGCTGG TCTGACGGGA TCCCATCGGA TGCCGAGACG 
GATCTCCGCG TGCAGCACCA GGAGGCGGTA GAGGCAGACC TGGAGCTGAG GGTCTCAGAG 
AATGAGGAGG AGCCACCTGA GGTCACACCA GGGCATCCGG AGAGAAGTCT CACCCGGGTC 
CCCAGCCCTG CCCTGTCCCC TGAGGAGCAA GCTGGTCTGC GGTCCCCAGG CCACAGCCCT 
AAGGCAGAGG GGGCCCAAAT CCCACTTGTC TCTGTTGTCA CCAAGGCAAA ATTGCCTGAA 
GAGAACCTTT TCCCCAAGCC TTTGCTCCCC AAAGTGAAGC CCAAGGTGGA AGTGCCCTGC 
AGTCAGAGAG CTGCATGCTC CCCCATCCGT TCTCAGCCAG TGGCTCTGCC AGAAGCCAGG 
ACACCTACCT CCCCAGTGAG CTCGCAGTGC CTGTCGCCTT TGGCCACCTC CACCCCCACC 
AGCACCCAGC TCCCCATTTG CTCCCAGCCT CAGCCTTCCT CTGAGACCAC GGCCCCATCC 
CCCACCAAGT CCCCCATACG GCTCCAGCCT GTTTCAGCCA AAACATCTAT CCCAGTGGTT 
CCCCTCCCCT TGAAGAACCA AGGGGACACC AAGGACAGGC TGGGCAGCCC TCTCGCTGTG 
GATGAGGCCC TGAAGCGGAA TGACCTGGTG GCAGAGTTCT GGATGAAGAG CGCCGAGATC 
CGCCGCAGCC TCGGGCTCAC ACCAGTGGAT CGGAGCAAGG TGTATGAGCC CAGCTTCCCT 
TCGCCTGCCT TTAAACCAGT GTCCCTAAAA TCCTATGCAG TGGAGAAGTC TCCCGAGGGG 
GAGGGACTCC AGCTTCTGAA GCCTCCACCT GTTCCTAAGA GACTAGGCCT GCCGAAGTCA 
GACGGTGACC AGCCCTTCCT GCCAACCCCC AAGTCCCCGT CGGACAGAGA GCTGAAAAGC 
TCCCAGGAGG AGAGGAGAGA TCTGTCGAGC AGCTCCGGGC TGGGCCTTCA CGGGAGCTCC 
TCCAACATGA AGACTTTGGG CAGCCAGAGC TTCAACACAT CAGACTCCAC CATGCTCACT 
CCCCCTTCAA GCCCACCGCC ACCACCACCC CAAGACGAGG AACCCGCAAC TCTTCGGAGG 
AAGCCATATC AGACTCATGA GCATAAGGAC GCTGAGCCCA AGGCCTCGGT GATTCCACCA 
CCACCACCTG CGACGTTCAT GCGGGCCCCC CGGGAGCCCA CCCAGCCCCT CCAAGAGGAG 
ATGCGGAAGT CGTTTGTGGA GAGCGTGGAT GAGATCCCAT TTGCCGATGA TGTGGAGGAC 
ACCTATGATG ACAAGACCGA GGACTCGAGT CTCCAGGAGA AGTTCTTCAC ACCCCCCTCC 
TACTGGCCCC GCACGGAGAG GCCCCTCCAC CCACCCCTAG CCAAGGAGAA TGGTAGGTTG 
CCTACTCTGG AGAGTGGGGT CCAGCCACAG AAGAGGGGCC TGCCCTTAGT CTCTCCAGAA 
GCCAAGGAGC TGGCTGAGGA GCGCATGCGA GCCAGGGAGA AGTCTGTGAA GAGCCAGGCG 
TTGAGAGACG CTATGGCCAA GCAGCTGAGT AAGATGAAGG AAATGGAGAT GGCGGCTGGG 
ACCTCCAGTC CCCCAGGAGG CACCTCCCAC AAAGCCTCCT CTGTTCCCTC CAAGGGCAAA 
GACCTCAGCC CCGAGTCCCC TAGACGCCCA GTTCTCAAAG GCCCCAGGGA GCCTGCCCTG 
AAGCACGAAG CCACTAGTGA GGAGGTCCTC TCCCCACCAT CGGACTCAGG GGGCCCAGAT 
GGTTCGGTCA CCTCATCCGA GGGCTCCAGT GGGAAGAGCA AGAAGAGATC GTCACTCTTC 
TCCCCCCGCA GAAACAAGAA AGAGAAGAAG TCCAAAGGTG AGGGCCGGCC CCCGGAGAAG 
CCCAGCCCAA GCCTTCTGGA GGAAGCTGCT GCCAAGCCCA AGTCCCTGTG GAAGTCGGTC 
TTTTCCGGGT ACAAGAAGGA CAAAAAGAAG AAGGGTGATG ACAAGTCCTG TTCCAGCACC 
CCTTCCAGCG GTGCCACTGT GGACTCTGGC AAGCACAAGA TGTCCCCTGT TGTGAGAGCA 
GAGCTGCACC TGCGGCGCCA GTTGAGCTTC TCCGAGGACT CAGACCTGTC CAGTGATGAC 
ATCCTTGAGA GGTCCTCCCA GAAGTCCAGG CGAGAGTCGG TTTATGTACC ACACGCCTTG 
GCATTCAAGA GATCATATCC ATCAAAGCCG AGAACCTACA CAGAGGAGGA ACTGAATGCC 
AAGCTGACCC GGCGTGTGCA GAAGGCAGCT CGTAGACAGG CCAAGCAAGA GGAGCTGAAG 
CGGCTGCACC GAGCACAGAT CATCCAGAGG CAGCTGGAGC AGGTGGAGGA GAAGCAGAGA 
CAGCTGGAGG AAAGAGGGGT GGCCGTGGAG AAGGCGCTCC GTGGTGAAGC AGACTATTGG 
GGAGAGTCTT ATTACAGTGA CCTCATCGAC TTGCATCTGG GTGTTGAGCC CAGTGGCGGG 
ACTCCACGGC GTAGACCTCT CTCCTTCTGC CCTTGCTGTG TCCAGGAAGG CATGGGCAAG 
AAGGATGACC CCAAGCTGAT GCAGGAGTGG TTCAAGTTGG TGCAAGAGAA GAATGCCATG 
GTGCGCTATG AGTCGGAGCT GATGATTTTT GCCCGGGAGC TGGAACTGGA GGACCGGCAG 
AGCCGACTGC AGCAGGAGCT CCGGGAGCGG ATGGCGGTGG AAGATCACCT GAAGACTGAG 
GAGGAGCTGT CTGAGGAGAA GAAGATCTTG AACGAGATGC TGGAGGTGGT GGAGCAGAGA 
GATGCCCTCG TGGCCCTGCT TGAGGAGCAG CGACTCCGGG AGAAGGAGGA GGACAAAGAC 
CTGGAGGCTG TCATGCTGTC CAAGGGCTTC AGCCTGAACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963858.1
CDS	245..7072
Translation	MEDSKNETVNHAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDYRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKACTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFQGLVQPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELKEATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLNYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREQNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNININFLRPSQVRHLYNTGETRDIHLEMENLVNSRTTPKLTRNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGKEMASVGEPDKLSMVMYLTQFYEMFKDSLPSSDALDLNAEEKAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEEAPRGYRGGRPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSTGMRRQPTQERGVSQPSCCLPEQGHPAPTPQWKQQQEKERSRTCPKKVITLSPPLTPPPCRAPRSQQTYRDLDADSRGKQSPHHERPEPEPSRRFFVDQWELSLSLRSSNRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCRKRVYVMERLSAEGKFFHRSCFKCEYCATTLRLSAYAYDIEDGKFYCKPHYCYRLSGSAQRKRPAVAPLSGKEAREPLQDGPTVDTNGRASAVTSPAERTPGSSVNGVEEPSIAKRLRGTPERIELENYRLSVKQAEELEEVPEETQAEHNLSSVLDTGAEEDVASSSSESEMEEEEEEEPPLPTSDLGGVPWKEAVRIHALLKGKSEEELEASKSFGPGHEEDEEEEEYEEEEEEEEEEYEEEEEEEESSEEGEYCPWDRELLQGQWLRQLSQEETTGTRKVGSQRLQQIVNPADPLEIQADVHWTHIREREEEERMVPTSESSTSRVPDLPSIRRAAVQAWLETVSGVPFDENDLEEDVDSEPAKIEGEATENGDTGDTGAELDDDQRWSDGIPSDAETDLRVQHQEAVEADLELRVSENEEEPPEVTPGHPERSLTRVPSPALSPEEQAGLRSPGHSPKAEGAQIPLVSVVTKAKLPEENLFPKPLLPKVKPKVEVPCSQRAACSPIRSQPVALPEARTPTSPVSSQCLSPLATSTPTSTQLPICSQPQPSSETTAPSPTKSPIRLQPVSAKTSIPVVPLPLKNQGDTKDRLGSPLAVDEALKRNDLVAEFWMKSAEIRRSLGLTPVDRSKVYEPSFPSPAFKPVSLKSYAVEKSPEGEGLQLLKPPPVPKRLGLPKSDGDQPFLPTPKSPSDRELKSSQEERRDLSSSSGLGLHGSSSNMKTLGSQSFNTSDSTMLTPPSSPPPPPPQDEEPATLRRKPYQTHEHKDAEPKASVIPPPPPATFMRAPREPTQPLQEEMRKSFVESVDEIPFADDVEDTYDDKTEDSSLQEKFFTPPSYWPRTERPLHPPLAKENGRLPTLESGVQPQKRGLPLVSPEAKELAEERMRAREKSVKSQALRDAMAKQLSKMKEMEMAAGTSSPPGGTSHKASSVPSKGKDLSPESPRRPVLKGPREPALKHEATSEEVLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRNKKEKKSKGEGRPPEKPSPSLLEEAAAKPKSLWKSVFSGYKKDKKKKGDDKSCSSTPSSGATVDSGKHKMSPVVRAELHLRRQLSFSEDSDLSSDDILERSSQKSRRESVYVPHALAFKRSYPSKPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELSEEKKILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAVMLSKGFSLNWS

Target ORF information:

RefSeq Version	XM_014108383.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108383.1

ORF Insert Sequence:

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTGCCTGAC CTTCCCTCCA TACGCCGCGC AGCAGTGCAG 
GCCTGGCTGG AGACGGTCTC TGGAGTCCCA TTTGATGAGA ATGACCTGGA GGAAGACGTG 
GACTCGGAGC CAGCAAAGAT AGAAGGGGAG GCCACAGAGA ATGGGGACAC AGGGGACACT 
GGTGCTGAGC TGGACGATGA TCAGCGCTGG TCTGACGGGA TCCCATCGGA TGCCGAGACG 
GATCTCCGCG TGCAGCACCA GGAGGCGGTA GAGGCAGACC TGGAGCTGAG GGTCTCAGAG 
AATGAGGAGG AGCCACCTGA GGTCACACCA GGGCATCCGG AGAGAAGTCT CACCCGGGTC 
CCCAGCCCTG CCCTGTCCCC TGAGGAGCAA GCTGGTCTGC GGTCCCCAGG CCACAGCCCT 
AAGGCAGAGG GGGCCCAAAT CCCACTTGTC TCTGTTGTCA CCAAGGCAAA ATTGCCTGAA 
GAGAACCTTT TCCCCAAGCC TTTGCTCCCC AAAGTGAAGC CCAAGGTGGA AGTGCCCTGC 
AGTCAGAGAG CTGCATGCTC CCCCATCCGT TCTCAGCCAG TGGCTCTGCC AGAAGCCAGG 
ACACCTACCT CCCCAGTGAG CTCGCAGTGC CTGTCGCCTT TGGCCACCTC CACCCCCACC 
AGCACCCAGC TCCCCATTTG CTCCCAGCCT CAGCCTTCCT CTGAGACCAC GGCCCCATCC 
CCCACCAAGT CCCCCATACG GCTCCAGCCT GTTTCAGCCA AAACATCTAT CCCAGTGGTT 
CCCCTCCCCT TGAAGAACCA AGGGGACACC AAGGACAGGC TGGGCAGCCC TCTCGCTGTG 
GATGAGGCCC TGAAGCGGAA TGACCTGGTG GCAGAGTTCT GGATGAAGAG CGCCGAGATC 
CGCCGCAGCC TCGGGCTCAC ACCAGTGGAT CGGAGCAAGG TGTATGAGCC CAGCTTCCCT 
TCGCCTGCCT TTAAACCAGT GTCCCTAAAA TCCTATGCAG TGGAGAAGTC TCCCGAGGGG 
GAGGGACTCC AGCTTCTGAA GCCTCCACCT GTTCCTAAGA GACTAGGCCT GCCGAAGTCA 
GACGGTGACC AGCCCTTCCT GCCAACCCCC AAGTCCCCGT CGGACAGAGA GCTGAAAAGC 
TCCCAGGAGG AGAGGAGAGA TCTGTCGAGC AGCTCCGGGC TGGGCCTTCA CGGGAGCTCC 
TCCAACATGA AGACTTTGGG CAGCCAGAGC TTCAACACAT CAGACTCCAC CATGCTCACT 
CCCCCTTCAA GCCCACCGCC ACCACCACCC CAAGACGAGG AACCCGCAAC TCTTCGGAGG 
AAGCCATATC AGACTCATGA GCATAAGGAC GCTGAGCCCA AGGCCTCGGT GATTCCACCA 
CCACCACCTG CGACGTTCAT GCGGGCCCCC CGGGAGCCCA CCCAGCCCCT CCAAGAGGAG 
ATGCGGAAGT CGTTTGTGGA GAGCGTGGAT GAGATCCCAT TTGCCGATGA TGTGGAGGAC 
ACCTATGATG ACAAGACCGA GGACTCGAGT CTCCAGGAGA AGTTCTTCAC ACCCCCCTCC 
TACTGGCCCC GCACGGAGAG GCCCCTCCAC CCACCCCTAG CCAAGGAGAA TGGTAGGTTG 
CCTACTCTGG AGAGTGGGGT CCAGCCACAG AAGAGGGGCC TGCCCTTAGT CTCTCCAGAA 
GCCAAGGAGC TGGCTGAGGA GCGCATGCGA GCCAGGGAGA AGTCTGTGAA GAGCCAGGCG 
TTGAGAGACG CTATGGCCAA GCAGCTGAGT AAGATGAAGG AAATGGAGAT GGCGGCTGGG 
ACCTCCAGTC CCCCAGGAGG CACCTCCCAC AAAGCCTCCT CTGTTCCCTC CAAGGGCAAA 
GACCTCAGCC CCGAGTCCCC TAGACGCCCA GTTCTCAAAG GCCCCAGGGA GCCTGCCCTG 
AAGCACGAAG CCACTAGTGA GGAGGTCCTC TCCCCACCAT CGGACTCAGG GGGCCCAGAT 
GGTTCGGTCA CCTCATCCGA GGGCTCCAGT GGGAAGAGCA AGAAGAGATC GTCACTCTTC 
TCCCCCCGCA GAAACAAGAA AGAGAAGAAG TCCAAAGGTG AGGGCCGGCC CCCGGAGAAG 
CCCAGCCCAA GCCTTCTGGA GGAAGCTGCT GCCAAGCCCA AGTCCCTGTG GAAGTCGGTC 
TTTTCCGGGT ACAAGAAGGA CAAAAAGAAG AAGGGTGATG ACAAGTCCTG TTCCAGCACC 
CCTTCCAGCG GTGCCACTGT GGACTCTGGC AAGCACAAGA TGTCCCCTGT TGTGAGAGCA 
GAGCTGCACC TGCGGCGCCA GTTGAGCTTC TCCGAGGACT CAGACCTGTC CAGTGATGAC 
ATCCTTGAGA GGTCCTCCCA GAAGTCCAGG CGAGAGTCGG TTTATGTACC ACACGCCTTG 
GCATTCAAGA GATCATATCC ATCAAAGCCG AGAACCTACA CAGAGGAGGA ACTGAATGCC 
AAGCTGACCC GGCGTGTGCA GAAGGCAGCT CGTAGACAGG CCAAGCAAGA GGAGCTGAAG 
CGGCTGCACC GAGCACAGAT CATCCAGAGG CAGCTGGAGC AGGTGGAGGA GAAGCAGAGA 
CAGCTGGAGG AAAGAGGGGT GGCCGTGGAG AAGGCGCTCC GTGGTGAAGC AGACTATTGG 
GGAGAGTCTT ATTACAGTGA CCTCATCGAC TTGCATCTGG GTGTTGAGCC CAGTGGCGGG 
ACTCCACGGC GTAGACCTCT CTCCTTCTGC CCTTGCTGTG TCCAGGAAGG CATGGGCAAG 
AAGGATGACC CCAAGCTGAT GCAGGAGTGG TTCAAGTTGG TGCAAGAGAA GAATGCCATG 
GTGCGCTATG AGTCGGAGCT GATGATTTTT GCCCGGGAGC TGGAACTGGA GGACCGGCAG 
AGCCGACTGC AGCAGGAGCT CCGGGAGCGG ATGGCGGTGG AAGATCACCT GAAGACTGAG 
GAGGAGCTGT CTGAGGAGAA GAAGATCTTG AACGAGATGC TGGAGGTGGT GGAGCAGAGA 
GATGCCCTCG TGGCCCTGCT TGAGGAGCAG CGACTCCGGG AGAAGGAGGA GGACAAAGAC 
CTGGAGGCTG TCATGCTGTC CAAGGGCTTC AGCCTGAACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_014108384.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963859.1 Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X2, mRNA.

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CloneID	OCe43613
Clone ID Related Accession (Same CDS sequence)	XM_014108387.2 , XM_014108388.2 , XM_014108383.1 , XM_014108384.1 , XM_014108383.2 , XM_022410874.1 , XM_014108386.1 , XM_014108386.2 , XM_022410875.1 , XM_014108385.1 , XM_014108387.1 , XM_014108388.1
Accession Version	XM_014108384.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTGCCTGAC CTTCCCTCCA TACGCCGCGC AGCAGTGCAG 
GCCTGGCTGG AGACGGTCTC TGGAGTCCCA TTTGATGAGA ATGACCTGGA GGAAGACGTG 
GACTCGGAGC CAGCAAAGAT AGAAGGGGAG GCCACAGAGA ATGGGGACAC AGGGGACACT 
GGTGCTGAGC TGGACGATGA TCAGCGCTGG TCTGACGGGA TCCCATCGGA TGCCGAGACG 
GATCTCCGCG TGCAGCACCA GGAGGCGGTA GAGGCAGACC TGGAGCTGAG GGTCTCAGAG 
AATGAGGAGG AGCCACCTGA GGTCACACCA GGGCATCCGG AGAGAAGTCT CACCCGGGTC 
CCCAGCCCTG CCCTGTCCCC TGAGGAGCAA GCTGGTCTGC GGTCCCCAGG CCACAGCCCT 
AAGGCAGAGG GGGCCCAAAT CCCACTTGTC TCTGTTGTCA CCAAGGCAAA ATTGCCTGAA 
GAGAACCTTT TCCCCAAGCC TTTGCTCCCC AAAGTGAAGC CCAAGGTGGA AGTGCCCTGC 
AGTCAGAGAG CTGCATGCTC CCCCATCCGT TCTCAGCCAG TGGCTCTGCC AGAAGCCAGG 
ACACCTACCT CCCCAGTGAG CTCGCAGTGC CTGTCGCCTT TGGCCACCTC CACCCCCACC 
AGCACCCAGC TCCCCATTTG CTCCCAGCCT CAGCCTTCCT CTGAGACCAC GGCCCCATCC 
CCCACCAAGT CCCCCATACG GCTCCAGCCT GTTTCAGCCA AAACATCTAT CCCAGTGGTT 
CCCCTCCCCT TGAAGAACCA AGGGGACACC AAGGACAGGC TGGGCAGCCC TCTCGCTGTG 
GATGAGGCCC TGAAGCGGAA TGACCTGGTG GCAGAGTTCT GGATGAAGAG CGCCGAGATC 
CGCCGCAGCC TCGGGCTCAC ACCAGTGGAT CGGAGCAAGG TGTATGAGCC CAGCTTCCCT 
TCGCCTGCCT TTAAACCAGT GTCCCTAAAA TCCTATGCAG TGGAGAAGTC TCCCGAGGGG 
GAGGGACTCC AGCTTCTGAA GCCTCCACCT GTTCCTAAGA GACTAGGCCT GCCGAAGTCA 
GACGGTGACC AGCCCTTCCT GCCAACCCCC AAGTCCCCGT CGGACAGAGA GCTGAAAAGC 
TCCCAGGAGG AGAGGAGAGA TCTGTCGAGC AGCTCCGGGC TGGGCCTTCA CGGGAGCTCC 
TCCAACATGA AGACTTTGGG CAGCCAGAGC TTCAACACAT CAGACTCCAC CATGCTCACT 
CCCCCTTCAA GCCCACCGCC ACCACCACCC CAAGACGAGG AACCCGCAAC TCTTCGGAGG 
AAGCCATATC AGACTCATGA GCATAAGGAC GCTGAGCCCA AGGCCTCGGT GATTCCACCA 
CCACCACCTG CGACGTTCAT GCGGGCCCCC CGGGAGCCCA CCCAGCCCCT CCAAGAGGAG 
ATGCGGAAGT CGTTTGTGGA GAGCGTGGAT GAGATCCCAT TTGCCGATGA TGTGGAGGAC 
ACCTATGATG ACAAGACCGA GGACTCGAGT CTCCAGGAGA AGTTCTTCAC ACCCCCCTCC 
TACTGGCCCC GCACGGAGAG GCCCCTCCAC CCACCCCTAG CCAAGGAGAA TGGTAGGTTG 
CCTACTCTGG AGAGTGGGGT CCAGCCACAG AAGAGGGGCC TGCCCTTAGT CTCTCCAGAA 
GCCAAGGAGC TGGCTGAGGA GCGCATGCGA GCCAGGGAGA AGTCTGTGAA GAGCCAGGCG 
TTGAGAGACG CTATGGCCAA GCAGCTGAGT AAGATGAAGG AAATGGAGAT GGCGGCTGGG 
ACCTCCAGTC CCCCAGGAGG CACCTCCCAC AAAGCCTCCT CTGTTCCCTC CAAGGGCAAA 
GACCTCAGCC CCGAGTCCCC TAGACGCCCA GTTCTCAAAG GCCCCAGGGA GCCTGCCCTG 
AAGCACGAAG CCACTAGTGA GGAGGTCCTC TCCCCACCAT CGGACTCAGG GGGCCCAGAT 
GGTTCGGTCA CCTCATCCGA GGGCTCCAGT GGGAAGAGCA AGAAGAGATC GTCACTCTTC 
TCCCCCCGCA GAAACAAGAA AGAGAAGAAG TCCAAAGGTG AGGGCCGGCC CCCGGAGAAG 
CCCAGCCCAA GCCTTCTGGA GGAAGCTGCT GCCAAGCCCA AGTCCCTGTG GAAGTCGGTC 
TTTTCCGGGT ACAAGAAGGA CAAAAAGAAG AAGGGTGATG ACAAGTCCTG TTCCAGCACC 
CCTTCCAGCG GTGCCACTGT GGACTCTGGC AAGCACAAGA TGTCCCCTGT TGTGAGAGCA 
GAGCTGCACC TGCGGCGCCA GTTGAGCTTC TCCGAGGACT CAGACCTGTC CAGTGATGAC 
ATCCTTGAGA GGTCCTCCCA GAAGTCCAGG CGAGAGTCGG TTTATGTACC ACACGCCTTG 
GCATTCAAGA GATCATATCC ATCAAAGCCG AGAACCTACA CAGAGGAGGA ACTGAATGCC 
AAGCTGACCC GGCGTGTGCA GAAGGCAGCT CGTAGACAGG CCAAGCAAGA GGAGCTGAAG 
CGGCTGCACC GAGCACAGAT CATCCAGAGG CAGCTGGAGC AGGTGGAGGA GAAGCAGAGA 
CAGCTGGAGG AAAGAGGGGT GGCCGTGGAG AAGGCGCTCC GTGGTGAAGC AGACTATTGG 
GGAGAGTCTT ATTACAGTGA CCTCATCGAC TTGCATCTGG GTGTTGAGCC CAGTGGCGGG 
ACTCCACGGC GTAGACCTCT CTCCTTCTGC CCTTGCTGTG TCCAGGAAGG CATGGGCAAG 
AAGGATGACC CCAAGCTGAT GCAGGAGTGG TTCAAGTTGG TGCAAGAGAA GAATGCCATG 
GTGCGCTATG AGTCGGAGCT GATGATTTTT GCCCGGGAGC TGGAACTGGA GGACCGGCAG 
AGCCGACTGC AGCAGGAGCT CCGGGAGCGG ATGGCGGTGG AAGATCACCT GAAGACTGAG 
GAGGAGCTGT CTGAGGAGAA GAAGATCTTG AACGAGATGC TGGAGGTGGT GGAGCAGAGA 
GATGCCCTCG TGGCCCTGCT TGAGGAGCAG CGACTCCGGG AGAAGGAGGA GGACAAAGAC 
CTGGAGGCTG TCATGCTGTC CAAGGGCTTC AGCCTGAACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963859.1
CDS	177..7004
Translation	MEDSKNETVNHAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDYRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKACTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFQGLVQPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELKEATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLNYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREQNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNININFLRPSQVRHLYNTGETRDIHLEMENLVNSRTTPKLTRNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGKEMASVGEPDKLSMVMYLTQFYEMFKDSLPSSDALDLNAEEKAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEEAPRGYRGGRPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSTGMRRQPTQERGVSQPSCCLPEQGHPAPTPQWKQQQEKERSRTCPKKVITLSPPLTPPPCRAPRSQQTYRDLDADSRGKQSPHHERPEPEPSRRFFVDQWELSLSLRSSNRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCRKRVYVMERLSAEGKFFHRSCFKCEYCATTLRLSAYAYDIEDGKFYCKPHYCYRLSGSAQRKRPAVAPLSGKEAREPLQDGPTVDTNGRASAVTSPAERTPGSSVNGVEEPSIAKRLRGTPERIELENYRLSVKQAEELEEVPEETQAEHNLSSVLDTGAEEDVASSSSESEMEEEEEEEPPLPTSDLGGVPWKEAVRIHALLKGKSEEELEASKSFGPGHEEDEEEEEYEEEEEEEEEEYEEEEEEEESSEEGEYCPWDRELLQGQWLRQLSQEETTGTRKVGSQRLQQIVNPADPLEIQADVHWTHIREREEEERMVPTSESSTSRVPDLPSIRRAAVQAWLETVSGVPFDENDLEEDVDSEPAKIEGEATENGDTGDTGAELDDDQRWSDGIPSDAETDLRVQHQEAVEADLELRVSENEEEPPEVTPGHPERSLTRVPSPALSPEEQAGLRSPGHSPKAEGAQIPLVSVVTKAKLPEENLFPKPLLPKVKPKVEVPCSQRAACSPIRSQPVALPEARTPTSPVSSQCLSPLATSTPTSTQLPICSQPQPSSETTAPSPTKSPIRLQPVSAKTSIPVVPLPLKNQGDTKDRLGSPLAVDEALKRNDLVAEFWMKSAEIRRSLGLTPVDRSKVYEPSFPSPAFKPVSLKSYAVEKSPEGEGLQLLKPPPVPKRLGLPKSDGDQPFLPTPKSPSDRELKSSQEERRDLSSSSGLGLHGSSSNMKTLGSQSFNTSDSTMLTPPSSPPPPPPQDEEPATLRRKPYQTHEHKDAEPKASVIPPPPPATFMRAPREPTQPLQEEMRKSFVESVDEIPFADDVEDTYDDKTEDSSLQEKFFTPPSYWPRTERPLHPPLAKENGRLPTLESGVQPQKRGLPLVSPEAKELAEERMRAREKSVKSQALRDAMAKQLSKMKEMEMAAGTSSPPGGTSHKASSVPSKGKDLSPESPRRPVLKGPREPALKHEATSEEVLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRNKKEKKSKGEGRPPEKPSPSLLEEAAAKPKSLWKSVFSGYKKDKKKKGDDKSCSSTPSSGATVDSGKHKMSPVVRAELHLRRQLSFSEDSDLSSDDILERSSQKSRRESVYVPHALAFKRSYPSKPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELSEEKKILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAVMLSKGFSLNWS

Target ORF information:

RefSeq Version	XM_014108384.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108384.1

ORF Insert Sequence:

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTGCCTGAC CTTCCCTCCA TACGCCGCGC AGCAGTGCAG 
GCCTGGCTGG AGACGGTCTC TGGAGTCCCA TTTGATGAGA ATGACCTGGA GGAAGACGTG 
GACTCGGAGC CAGCAAAGAT AGAAGGGGAG GCCACAGAGA ATGGGGACAC AGGGGACACT 
GGTGCTGAGC TGGACGATGA TCAGCGCTGG TCTGACGGGA TCCCATCGGA TGCCGAGACG 
GATCTCCGCG TGCAGCACCA GGAGGCGGTA GAGGCAGACC TGGAGCTGAG GGTCTCAGAG 
AATGAGGAGG AGCCACCTGA GGTCACACCA GGGCATCCGG AGAGAAGTCT CACCCGGGTC 
CCCAGCCCTG CCCTGTCCCC TGAGGAGCAA GCTGGTCTGC GGTCCCCAGG CCACAGCCCT 
AAGGCAGAGG GGGCCCAAAT CCCACTTGTC TCTGTTGTCA CCAAGGCAAA ATTGCCTGAA 
GAGAACCTTT TCCCCAAGCC TTTGCTCCCC AAAGTGAAGC CCAAGGTGGA AGTGCCCTGC 
AGTCAGAGAG CTGCATGCTC CCCCATCCGT TCTCAGCCAG TGGCTCTGCC AGAAGCCAGG 
ACACCTACCT CCCCAGTGAG CTCGCAGTGC CTGTCGCCTT TGGCCACCTC CACCCCCACC 
AGCACCCAGC TCCCCATTTG CTCCCAGCCT CAGCCTTCCT CTGAGACCAC GGCCCCATCC 
CCCACCAAGT CCCCCATACG GCTCCAGCCT GTTTCAGCCA AAACATCTAT CCCAGTGGTT 
CCCCTCCCCT TGAAGAACCA AGGGGACACC AAGGACAGGC TGGGCAGCCC TCTCGCTGTG 
GATGAGGCCC TGAAGCGGAA TGACCTGGTG GCAGAGTTCT GGATGAAGAG CGCCGAGATC 
CGCCGCAGCC TCGGGCTCAC ACCAGTGGAT CGGAGCAAGG TGTATGAGCC CAGCTTCCCT 
TCGCCTGCCT TTAAACCAGT GTCCCTAAAA TCCTATGCAG TGGAGAAGTC TCCCGAGGGG 
GAGGGACTCC AGCTTCTGAA GCCTCCACCT GTTCCTAAGA GACTAGGCCT GCCGAAGTCA 
GACGGTGACC AGCCCTTCCT GCCAACCCCC AAGTCCCCGT CGGACAGAGA GCTGAAAAGC 
TCCCAGGAGG AGAGGAGAGA TCTGTCGAGC AGCTCCGGGC TGGGCCTTCA CGGGAGCTCC 
TCCAACATGA AGACTTTGGG CAGCCAGAGC TTCAACACAT CAGACTCCAC CATGCTCACT 
CCCCCTTCAA GCCCACCGCC ACCACCACCC CAAGACGAGG AACCCGCAAC TCTTCGGAGG 
AAGCCATATC AGACTCATGA GCATAAGGAC GCTGAGCCCA AGGCCTCGGT GATTCCACCA 
CCACCACCTG CGACGTTCAT GCGGGCCCCC CGGGAGCCCA CCCAGCCCCT CCAAGAGGAG 
ATGCGGAAGT CGTTTGTGGA GAGCGTGGAT GAGATCCCAT TTGCCGATGA TGTGGAGGAC 
ACCTATGATG ACAAGACCGA GGACTCGAGT CTCCAGGAGA AGTTCTTCAC ACCCCCCTCC 
TACTGGCCCC GCACGGAGAG GCCCCTCCAC CCACCCCTAG CCAAGGAGAA TGGTAGGTTG 
CCTACTCTGG AGAGTGGGGT CCAGCCACAG AAGAGGGGCC TGCCCTTAGT CTCTCCAGAA 
GCCAAGGAGC TGGCTGAGGA GCGCATGCGA GCCAGGGAGA AGTCTGTGAA GAGCCAGGCG 
TTGAGAGACG CTATGGCCAA GCAGCTGAGT AAGATGAAGG AAATGGAGAT GGCGGCTGGG 
ACCTCCAGTC CCCCAGGAGG CACCTCCCAC AAAGCCTCCT CTGTTCCCTC CAAGGGCAAA 
GACCTCAGCC CCGAGTCCCC TAGACGCCCA GTTCTCAAAG GCCCCAGGGA GCCTGCCCTG 
AAGCACGAAG CCACTAGTGA GGAGGTCCTC TCCCCACCAT CGGACTCAGG GGGCCCAGAT 
GGTTCGGTCA CCTCATCCGA GGGCTCCAGT GGGAAGAGCA AGAAGAGATC GTCACTCTTC 
TCCCCCCGCA GAAACAAGAA AGAGAAGAAG TCCAAAGGTG AGGGCCGGCC CCCGGAGAAG 
CCCAGCCCAA GCCTTCTGGA GGAAGCTGCT GCCAAGCCCA AGTCCCTGTG GAAGTCGGTC 
TTTTCCGGGT ACAAGAAGGA CAAAAAGAAG AAGGGTGATG ACAAGTCCTG TTCCAGCACC 
CCTTCCAGCG GTGCCACTGT GGACTCTGGC AAGCACAAGA TGTCCCCTGT TGTGAGAGCA 
GAGCTGCACC TGCGGCGCCA GTTGAGCTTC TCCGAGGACT CAGACCTGTC CAGTGATGAC 
ATCCTTGAGA GGTCCTCCCA GAAGTCCAGG CGAGAGTCGG TTTATGTACC ACACGCCTTG 
GCATTCAAGA GATCATATCC ATCAAAGCCG AGAACCTACA CAGAGGAGGA ACTGAATGCC 
AAGCTGACCC GGCGTGTGCA GAAGGCAGCT CGTAGACAGG CCAAGCAAGA GGAGCTGAAG 
CGGCTGCACC GAGCACAGAT CATCCAGAGG CAGCTGGAGC AGGTGGAGGA GAAGCAGAGA 
CAGCTGGAGG AAAGAGGGGT GGCCGTGGAG AAGGCGCTCC GTGGTGAAGC AGACTATTGG 
GGAGAGTCTT ATTACAGTGA CCTCATCGAC TTGCATCTGG GTGTTGAGCC CAGTGGCGGG 
ACTCCACGGC GTAGACCTCT CTCCTTCTGC CCTTGCTGTG TCCAGGAAGG CATGGGCAAG 
AAGGATGACC CCAAGCTGAT GCAGGAGTGG TTCAAGTTGG TGCAAGAGAA GAATGCCATG 
GTGCGCTATG AGTCGGAGCT GATGATTTTT GCCCGGGAGC TGGAACTGGA GGACCGGCAG 
AGCCGACTGC AGCAGGAGCT CCGGGAGCGG ATGGCGGTGG AAGATCACCT GAAGACTGAG 
GAGGAGCTGT CTGAGGAGAA GAAGATCTTG AACGAGATGC TGGAGGTGGT GGAGCAGAGA 
GATGCCCTCG TGGCCCTGCT TGAGGAGCAG CGACTCCGGG AGAAGGAGGA GGACAAAGAC 
CTGGAGGCTG TCATGCTGTC CAAGGGCTTC AGCCTGAACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_014108383.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963858.1 Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X1, mRNA.

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CloneID	OCe43613
Clone ID Related Accession (Same CDS sequence)	XM_014108387.2 , XM_014108388.2 , XM_014108383.1 , XM_014108384.1 , XM_014108383.2 , XM_022410874.1 , XM_014108386.1 , XM_014108386.2 , XM_022410875.1 , XM_014108385.1 , XM_014108387.1 , XM_014108388.1
Accession Version	XM_014108383.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	F-actin-methionine sulfoxide oxidase MICAL3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014108383.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTGCCTGAC CTTCCCTCCA TACGCCGCGC AGCAGTGCAG 
GCCTGGCTGG AGACGGTCTC TGGAGTCCCA TTTGATGAGA ATGACCTGGA GGAAGACGTG 
GACTCGGAGC CAGCAAAGAT AGAAGGGGAG GCCACAGAGA ATGGGGACAC AGGGGACACT 
GGTGCTGAGC TGGACGATGA TCAGCGCTGG TCTGACGGGA TCCCATCGGA TGCCGAGACG 
GATCTCCGCG TGCAGCACCA GGAGGCGGTA GAGGCAGACC TGGAGCTGAG GGTCTCAGAG 
AATGAGGAGG AGCCACCTGA GGTCACACCA GGGCATCCGG AGAGAAGTCT CACCCGGGTC 
CCCAGCCCTG CCCTGTCCCC TGAGGAGCAA GCTGGTCTGC GGTCCCCAGG CCACAGCCCT 
AAGGCAGAGG GGGCCCAAAT CCCACTTGTC TCTGTTGTCA CCAAGGCAAA ATTGCCTGAA 
GAGAACCTTT TCCCCAAGCC TTTGCTCCCC AAAGTGAAGC CCAAGGTGGA AGTGCCCTGC 
AGTCAGAGAG CTGCATGCTC CCCCATCCGT TCTCAGCCAG TGGCTCTGCC AGAAGCCAGG 
ACACCTACCT CCCCAGTGAG CTCGCAGTGC CTGTCGCCTT TGGCCACCTC CACCCCCACC 
AGCACCCAGC TCCCCATTTG CTCCCAGCCT CAGCCTTCCT CTGAGACCAC GGCCCCATCC 
CCCACCAAGT CCCCCATACG GCTCCAGCCT GTTTCAGCCA AAACATCTAT CCCAGTGGTT 
CCCCTCCCCT TGAAGAACCA AGGGGACACC AAGGACAGGC TGGGCAGCCC TCTCGCTGTG 
GATGAGGCCC TGAAGCGGAA TGACCTGGTG GCAGAGTTCT GGATGAAGAG CGCCGAGATC 
CGCCGCAGCC TCGGGCTCAC ACCAGTGGAT CGGAGCAAGG TGTATGAGCC CAGCTTCCCT 
TCGCCTGCCT TTAAACCAGT GTCCCTAAAA TCCTATGCAG TGGAGAAGTC TCCCGAGGGG 
GAGGGACTCC AGCTTCTGAA GCCTCCACCT GTTCCTAAGA GACTAGGCCT GCCGAAGTCA 
GACGGTGACC AGCCCTTCCT GCCAACCCCC AAGTCCCCGT CGGACAGAGA GCTGAAAAGC 
TCCCAGGAGG AGAGGAGAGA TCTGTCGAGC AGCTCCGGGC TGGGCCTTCA CGGGAGCTCC 
TCCAACATGA AGACTTTGGG CAGCCAGAGC TTCAACACAT CAGACTCCAC CATGCTCACT 
CCCCCTTCAA GCCCACCGCC ACCACCACCC CAAGACGAGG AACCCGCAAC TCTTCGGAGG 
AAGCCATATC AGACTCATGA GCATAAGGAC GCTGAGCCCA AGGCCTCGGT GATTCCACCA 
CCACCACCTG CGACGTTCAT GCGGGCCCCC CGGGAGCCCA CCCAGCCCCT CCAAGAGGAG 
ATGCGGAAGT CGTTTGTGGA GAGCGTGGAT GAGATCCCAT TTGCCGATGA TGTGGAGGAC 
ACCTATGATG ACAAGACCGA GGACTCGAGT CTCCAGGAGA AGTTCTTCAC ACCCCCCTCC 
TACTGGCCCC GCACGGAGAG GCCCCTCCAC CCACCCCTAG CCAAGGAGAA TGGTAGGTTG 
CCTACTCTGG AGAGTGGGGT CCAGCCACAG AAGAGGGGCC TGCCCTTAGT CTCTCCAGAA 
GCCAAGGAGC TGGCTGAGGA GCGCATGCGA GCCAGGGAGA AGTCTGTGAA GAGCCAGGCG 
TTGAGAGACG CTATGGCCAA GCAGCTGAGT AAGATGAAGG AAATGGAGAT GGCGGCTGGG 
ACCTCCAGTC CCCCAGGAGG CACCTCCCAC AAAGCCTCCT CTGTTCCCTC CAAGGGCAAA 
GACCTCAGCC CCGAGTCCCC TAGACGCCCA GTTCTCAAAG GCCCCAGGGA GCCTGCCCTG 
AAGCACGAAG CCACTAGTGA GGAGGTCCTC TCCCCACCAT CGGACTCAGG GGGCCCAGAT 
GGTTCGGTCA CCTCATCCGA GGGCTCCAGT GGGAAGAGCA AGAAGAGATC GTCACTCTTC 
TCCCCCCGCA GAAACAAGAA AGAGAAGAAG TCCAAAGGTG AGGGCCGGCC CCCGGAGAAG 
CCCAGCCCAA GCCTTCTGGA GGAAGCTGCT GCCAAGCCCA AGTCCCTGTG GAAGTCGGTC 
TTTTCCGGGT ACAAGAAGGA CAAAAAGAAG AAGGGTGATG ACAAGTCCTG TTCCAGCACC 
CCTTCCAGCG GTGCCACTGT GGACTCTGGC AAGCACAAGA TGTCCCCTGT TGTGAGAGCA 
GAGCTGCACC TGCGGCGCCA GTTGAGCTTC TCCGAGGACT CAGACCTGTC CAGTGATGAC 
ATCCTTGAGA GGTCCTCCCA GAAGTCCAGG CGAGAGTCGG TTTATGTACC ACACGCCTTG 
GCATTCAAGA GATCATATCC ATCAAAGCCG AGAACCTACA CAGAGGAGGA ACTGAATGCC 
AAGCTGACCC GGCGTGTGCA GAAGGCAGCT CGTAGACAGG CCAAGCAAGA GGAGCTGAAG 
CGGCTGCACC GAGCACAGAT CATCCAGAGG CAGCTGGAGC AGGTGGAGGA GAAGCAGAGA 
CAGCTGGAGG AAAGAGGGGT GGCCGTGGAG AAGGCGCTCC GTGGTGAAGC AGACTATTGG 
GGAGAGTCTT ATTACAGTGA CCTCATCGAC TTGCATCTGG GTGTTGAGCC CAGTGGCGGG 
ACTCCACGGC GTAGACCTCT CTCCTTCTGC CCTTGCTGTG TCCAGGAAGG CATGGGCAAG 
AAGGATGACC CCAAGCTGAT GCAGGAGTGG TTCAAGTTGG TGCAAGAGAA GAATGCCATG 
GTGCGCTATG AGTCGGAGCT GATGATTTTT GCCCGGGAGC TGGAACTGGA GGACCGGCAG 
AGCCGACTGC AGCAGGAGCT CCGGGAGCGG ATGGCGGTGG AAGATCACCT GAAGACTGAG 
GAGGAGCTGT CTGAGGAGAA GAAGATCTTG AACGAGATGC TGGAGGTGGT GGAGCAGAGA 
GATGCCCTCG TGGCCCTGCT TGAGGAGCAG CGACTCCGGG AGAAGGAGGA GGACAAAGAC 
CTGGAGGCTG TCATGCTGTC CAAGGGCTTC AGCCTGAACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963858.1
CDS	258..7085
Translation	MEDSKNETVNHAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDYRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKACTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFQGLVQPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELKEATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLNYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREQNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNININFLRPSQVRHLYNTGETRDIHLEMENLVNSRTTPKLTRNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGKEMASVGEPDKLSMVMYLTQFYEMFKDSLPSSDALDLNAEEKAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEEAPRGYRGGRPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSTGMRRQPTQERGVSQPSCCLPEQGHPAPTPQWKQQQEKERSRTCPKKVITLSPPLTPPPCRAPRSQQTYRDLDADSRGKQSPHHERPEPEPSRRFFVDQWELSLSLRSSNRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCRKRVYVMERLSAEGKFFHRSCFKCEYCATTLRLSAYAYDIEDGKFYCKPHYCYRLSGSAQRKRPAVAPLSGKEAREPLQDGPTVDTNGRASAVTSPAERTPGSSVNGVEEPSIAKRLRGTPERIELENYRLSVKQAEELEEVPEETQAEHNLSSVLDTGAEEDVASSSSESEMEEEEEEEPPLPTSDLGGVPWKEAVRIHALLKGKSEEELEASKSFGPGHEEDEEEEEYEEEEEEEEEEYEEEEEEEESSEEGEYCPWDRELLQGQWLRQLSQEETTGTRKVGSQRLQQIVNPADPLEIQADVHWTHIREREEEERMVPTSESSTSRVPDLPSIRRAAVQAWLETVSGVPFDENDLEEDVDSEPAKIEGEATENGDTGDTGAELDDDQRWSDGIPSDAETDLRVQHQEAVEADLELRVSENEEEPPEVTPGHPERSLTRVPSPALSPEEQAGLRSPGHSPKAEGAQIPLVSVVTKAKLPEENLFPKPLLPKVKPKVEVPCSQRAACSPIRSQPVALPEARTPTSPVSSQCLSPLATSTPTSTQLPICSQPQPSSETTAPSPTKSPIRLQPVSAKTSIPVVPLPLKNQGDTKDRLGSPLAVDEALKRNDLVAEFWMKSAEIRRSLGLTPVDRSKVYEPSFPSPAFKPVSLKSYAVEKSPEGEGLQLLKPPPVPKRLGLPKSDGDQPFLPTPKSPSDRELKSSQEERRDLSSSSGLGLHGSSSNMKTLGSQSFNTSDSTMLTPPSSPPPPPPQDEEPATLRRKPYQTHEHKDAEPKASVIPPPPPATFMRAPREPTQPLQEEMRKSFVESVDEIPFADDVEDTYDDKTEDSSLQEKFFTPPSYWPRTERPLHPPLAKENGRLPTLESGVQPQKRGLPLVSPEAKELAEERMRAREKSVKSQALRDAMAKQLSKMKEMEMAAGTSSPPGGTSHKASSVPSKGKDLSPESPRRPVLKGPREPALKHEATSEEVLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRNKKEKKSKGEGRPPEKPSPSLLEEAAAKPKSLWKSVFSGYKKDKKKKGDDKSCSSTPSSGATVDSGKHKMSPVVRAELHLRRQLSFSEDSDLSSDDILERSSQKSRRESVYVPHALAFKRSYPSKPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELSEEKKILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAVMLSKGFSLNWS

Target ORF information:

RefSeq Version	XM_014108383.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108383.2

ORF Insert Sequence:

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTGCCTGAC CTTCCCTCCA TACGCCGCGC AGCAGTGCAG 
GCCTGGCTGG AGACGGTCTC TGGAGTCCCA TTTGATGAGA ATGACCTGGA GGAAGACGTG 
GACTCGGAGC CAGCAAAGAT AGAAGGGGAG GCCACAGAGA ATGGGGACAC AGGGGACACT 
GGTGCTGAGC TGGACGATGA TCAGCGCTGG TCTGACGGGA TCCCATCGGA TGCCGAGACG 
GATCTCCGCG TGCAGCACCA GGAGGCGGTA GAGGCAGACC TGGAGCTGAG GGTCTCAGAG 
AATGAGGAGG AGCCACCTGA GGTCACACCA GGGCATCCGG AGAGAAGTCT CACCCGGGTC 
CCCAGCCCTG CCCTGTCCCC TGAGGAGCAA GCTGGTCTGC GGTCCCCAGG CCACAGCCCT 
AAGGCAGAGG GGGCCCAAAT CCCACTTGTC TCTGTTGTCA CCAAGGCAAA ATTGCCTGAA 
GAGAACCTTT TCCCCAAGCC TTTGCTCCCC AAAGTGAAGC CCAAGGTGGA AGTGCCCTGC 
AGTCAGAGAG CTGCATGCTC CCCCATCCGT TCTCAGCCAG TGGCTCTGCC AGAAGCCAGG 
ACACCTACCT CCCCAGTGAG CTCGCAGTGC CTGTCGCCTT TGGCCACCTC CACCCCCACC 
AGCACCCAGC TCCCCATTTG CTCCCAGCCT CAGCCTTCCT CTGAGACCAC GGCCCCATCC 
CCCACCAAGT CCCCCATACG GCTCCAGCCT GTTTCAGCCA AAACATCTAT CCCAGTGGTT 
CCCCTCCCCT TGAAGAACCA AGGGGACACC AAGGACAGGC TGGGCAGCCC TCTCGCTGTG 
GATGAGGCCC TGAAGCGGAA TGACCTGGTG GCAGAGTTCT GGATGAAGAG CGCCGAGATC 
CGCCGCAGCC TCGGGCTCAC ACCAGTGGAT CGGAGCAAGG TGTATGAGCC CAGCTTCCCT 
TCGCCTGCCT TTAAACCAGT GTCCCTAAAA TCCTATGCAG TGGAGAAGTC TCCCGAGGGG 
GAGGGACTCC AGCTTCTGAA GCCTCCACCT GTTCCTAAGA GACTAGGCCT GCCGAAGTCA 
GACGGTGACC AGCCCTTCCT GCCAACCCCC AAGTCCCCGT CGGACAGAGA GCTGAAAAGC 
TCCCAGGAGG AGAGGAGAGA TCTGTCGAGC AGCTCCGGGC TGGGCCTTCA CGGGAGCTCC 
TCCAACATGA AGACTTTGGG CAGCCAGAGC TTCAACACAT CAGACTCCAC CATGCTCACT 
CCCCCTTCAA GCCCACCGCC ACCACCACCC CAAGACGAGG AACCCGCAAC TCTTCGGAGG 
AAGCCATATC AGACTCATGA GCATAAGGAC GCTGAGCCCA AGGCCTCGGT GATTCCACCA 
CCACCACCTG CGACGTTCAT GCGGGCCCCC CGGGAGCCCA CCCAGCCCCT CCAAGAGGAG 
ATGCGGAAGT CGTTTGTGGA GAGCGTGGAT GAGATCCCAT TTGCCGATGA TGTGGAGGAC 
ACCTATGATG ACAAGACCGA GGACTCGAGT CTCCAGGAGA AGTTCTTCAC ACCCCCCTCC 
TACTGGCCCC GCACGGAGAG GCCCCTCCAC CCACCCCTAG CCAAGGAGAA TGGTAGGTTG 
CCTACTCTGG AGAGTGGGGT CCAGCCACAG AAGAGGGGCC TGCCCTTAGT CTCTCCAGAA 
GCCAAGGAGC TGGCTGAGGA GCGCATGCGA GCCAGGGAGA AGTCTGTGAA GAGCCAGGCG 
TTGAGAGACG CTATGGCCAA GCAGCTGAGT AAGATGAAGG AAATGGAGAT GGCGGCTGGG 
ACCTCCAGTC CCCCAGGAGG CACCTCCCAC AAAGCCTCCT CTGTTCCCTC CAAGGGCAAA 
GACCTCAGCC CCGAGTCCCC TAGACGCCCA GTTCTCAAAG GCCCCAGGGA GCCTGCCCTG 
AAGCACGAAG CCACTAGTGA GGAGGTCCTC TCCCCACCAT CGGACTCAGG GGGCCCAGAT 
GGTTCGGTCA CCTCATCCGA GGGCTCCAGT GGGAAGAGCA AGAAGAGATC GTCACTCTTC 
TCCCCCCGCA GAAACAAGAA AGAGAAGAAG TCCAAAGGTG AGGGCCGGCC CCCGGAGAAG 
CCCAGCCCAA GCCTTCTGGA GGAAGCTGCT GCCAAGCCCA AGTCCCTGTG GAAGTCGGTC 
TTTTCCGGGT ACAAGAAGGA CAAAAAGAAG AAGGGTGATG ACAAGTCCTG TTCCAGCACC 
CCTTCCAGCG GTGCCACTGT GGACTCTGGC AAGCACAAGA TGTCCCCTGT TGTGAGAGCA 
GAGCTGCACC TGCGGCGCCA GTTGAGCTTC TCCGAGGACT CAGACCTGTC CAGTGATGAC 
ATCCTTGAGA GGTCCTCCCA GAAGTCCAGG CGAGAGTCGG TTTATGTACC ACACGCCTTG 
GCATTCAAGA GATCATATCC ATCAAAGCCG AGAACCTACA CAGAGGAGGA ACTGAATGCC 
AAGCTGACCC GGCGTGTGCA GAAGGCAGCT CGTAGACAGG CCAAGCAAGA GGAGCTGAAG 
CGGCTGCACC GAGCACAGAT CATCCAGAGG CAGCTGGAGC AGGTGGAGGA GAAGCAGAGA 
CAGCTGGAGG AAAGAGGGGT GGCCGTGGAG AAGGCGCTCC GTGGTGAAGC AGACTATTGG 
GGAGAGTCTT ATTACAGTGA CCTCATCGAC TTGCATCTGG GTGTTGAGCC CAGTGGCGGG 
ACTCCACGGC GTAGACCTCT CTCCTTCTGC CCTTGCTGTG TCCAGGAAGG CATGGGCAAG 
AAGGATGACC CCAAGCTGAT GCAGGAGTGG TTCAAGTTGG TGCAAGAGAA GAATGCCATG 
GTGCGCTATG AGTCGGAGCT GATGATTTTT GCCCGGGAGC TGGAACTGGA GGACCGGCAG 
AGCCGACTGC AGCAGGAGCT CCGGGAGCGG ATGGCGGTGG AAGATCACCT GAAGACTGAG 
GAGGAGCTGT CTGAGGAGAA GAAGATCTTG AACGAGATGC TGGAGGTGGT GGAGCAGAGA 
GATGCCCTCG TGGCCCTGCT TGAGGAGCAG CGACTCCGGG AGAAGGAGGA GGACAAAGAC 
CTGGAGGCTG TCATGCTGTC CAAGGGCTTC AGCCTGAACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_022410874.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022266582.1 Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X2, mRNA.

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CloneID	OCe43613
Clone ID Related Accession (Same CDS sequence)	XM_014108387.2 , XM_014108388.2 , XM_014108383.1 , XM_014108384.1 , XM_014108383.2 , XM_022410874.1 , XM_014108386.1 , XM_014108386.2 , XM_022410875.1 , XM_014108385.1 , XM_014108387.1 , XM_014108388.1
Accession Version	XM_022410874.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	F-actin-methionine sulfoxide oxidase MICAL3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTGCCTGAC CTTCCCTCCA TACGCCGCGC AGCAGTGCAG 
GCCTGGCTGG AGACGGTCTC TGGAGTCCCA TTTGATGAGA ATGACCTGGA GGAAGACGTG 
GACTCGGAGC CAGCAAAGAT AGAAGGGGAG GCCACAGAGA ATGGGGACAC AGGGGACACT 
GGTGCTGAGC TGGACGATGA TCAGCGCTGG TCTGACGGGA TCCCATCGGA TGCCGAGACG 
GATCTCCGCG TGCAGCACCA GGAGGCGGTA GAGGCAGACC TGGAGCTGAG GGTCTCAGAG 
AATGAGGAGG AGCCACCTGA GGTCACACCA GGGCATCCGG AGAGAAGTCT CACCCGGGTC 
CCCAGCCCTG CCCTGTCCCC TGAGGAGCAA GCTGGTCTGC GGTCCCCAGG CCACAGCCCT 
AAGGCAGAGG GGGCCCAAAT CCCACTTGTC TCTGTTGTCA CCAAGGCAAA ATTGCCTGAA 
GAGAACCTTT TCCCCAAGCC TTTGCTCCCC AAAGTGAAGC CCAAGGTGGA AGTGCCCTGC 
AGTCAGAGAG CTGCATGCTC CCCCATCCGT TCTCAGCCAG TGGCTCTGCC AGAAGCCAGG 
ACACCTACCT CCCCAGTGAG CTCGCAGTGC CTGTCGCCTT TGGCCACCTC CACCCCCACC 
AGCACCCAGC TCCCCATTTG CTCCCAGCCT CAGCCTTCCT CTGAGACCAC GGCCCCATCC 
CCCACCAAGT CCCCCATACG GCTCCAGCCT GTTTCAGCCA AAACATCTAT CCCAGTGGTT 
CCCCTCCCCT TGAAGAACCA AGGGGACACC AAGGACAGGC TGGGCAGCCC TCTCGCTGTG 
GATGAGGCCC TGAAGCGGAA TGACCTGGTG GCAGAGTTCT GGATGAAGAG CGCCGAGATC 
CGCCGCAGCC TCGGGCTCAC ACCAGTGGAT CGGAGCAAGG TGTATGAGCC CAGCTTCCCT 
TCGCCTGCCT TTAAACCAGT GTCCCTAAAA TCCTATGCAG TGGAGAAGTC TCCCGAGGGG 
GAGGGACTCC AGCTTCTGAA GCCTCCACCT GTTCCTAAGA GACTAGGCCT GCCGAAGTCA 
GACGGTGACC AGCCCTTCCT GCCAACCCCC AAGTCCCCGT CGGACAGAGA GCTGAAAAGC 
TCCCAGGAGG AGAGGAGAGA TCTGTCGAGC AGCTCCGGGC TGGGCCTTCA CGGGAGCTCC 
TCCAACATGA AGACTTTGGG CAGCCAGAGC TTCAACACAT CAGACTCCAC CATGCTCACT 
CCCCCTTCAA GCCCACCGCC ACCACCACCC CAAGACGAGG AACCCGCAAC TCTTCGGAGG 
AAGCCATATC AGACTCATGA GCATAAGGAC GCTGAGCCCA AGGCCTCGGT GATTCCACCA 
CCACCACCTG CGACGTTCAT GCGGGCCCCC CGGGAGCCCA CCCAGCCCCT CCAAGAGGAG 
ATGCGGAAGT CGTTTGTGGA GAGCGTGGAT GAGATCCCAT TTGCCGATGA TGTGGAGGAC 
ACCTATGATG ACAAGACCGA GGACTCGAGT CTCCAGGAGA AGTTCTTCAC ACCCCCCTCC 
TACTGGCCCC GCACGGAGAG GCCCCTCCAC CCACCCCTAG CCAAGGAGAA TGGTAGGTTG 
CCTACTCTGG AGAGTGGGGT CCAGCCACAG AAGAGGGGCC TGCCCTTAGT CTCTCCAGAA 
GCCAAGGAGC TGGCTGAGGA GCGCATGCGA GCCAGGGAGA AGTCTGTGAA GAGCCAGGCG 
TTGAGAGACG CTATGGCCAA GCAGCTGAGT AAGATGAAGG AAATGGAGAT GGCGGCTGGG 
ACCTCCAGTC CCCCAGGAGG CACCTCCCAC AAAGCCTCCT CTGTTCCCTC CAAGGGCAAA 
GACCTCAGCC CCGAGTCCCC TAGACGCCCA GTTCTCAAAG GCCCCAGGGA GCCTGCCCTG 
AAGCACGAAG CCACTAGTGA GGAGGTCCTC TCCCCACCAT CGGACTCAGG GGGCCCAGAT 
GGTTCGGTCA CCTCATCCGA GGGCTCCAGT GGGAAGAGCA AGAAGAGATC GTCACTCTTC 
TCCCCCCGCA GAAACAAGAA AGAGAAGAAG TCCAAAGGTG AGGGCCGGCC CCCGGAGAAG 
CCCAGCCCAA GCCTTCTGGA GGAAGCTGCT GCCAAGCCCA AGTCCCTGTG GAAGTCGGTC 
TTTTCCGGGT ACAAGAAGGA CAAAAAGAAG AAGGGTGATG ACAAGTCCTG TTCCAGCACC 
CCTTCCAGCG GTGCCACTGT GGACTCTGGC AAGCACAAGA TGTCCCCTGT TGTGAGAGCA 
GAGCTGCACC TGCGGCGCCA GTTGAGCTTC TCCGAGGACT CAGACCTGTC CAGTGATGAC 
ATCCTTGAGA GGTCCTCCCA GAAGTCCAGG CGAGAGTCGG TTTATGTACC ACACGCCTTG 
GCATTCAAGA GATCATATCC ATCAAAGCCG AGAACCTACA CAGAGGAGGA ACTGAATGCC 
AAGCTGACCC GGCGTGTGCA GAAGGCAGCT CGTAGACAGG CCAAGCAAGA GGAGCTGAAG 
CGGCTGCACC GAGCACAGAT CATCCAGAGG CAGCTGGAGC AGGTGGAGGA GAAGCAGAGA 
CAGCTGGAGG AAAGAGGGGT GGCCGTGGAG AAGGCGCTCC GTGGTGAAGC AGACTATTGG 
GGAGAGTCTT ATTACAGTGA CCTCATCGAC TTGCATCTGG GTGTTGAGCC CAGTGGCGGG 
ACTCCACGGC GTAGACCTCT CTCCTTCTGC CCTTGCTGTG TCCAGGAAGG CATGGGCAAG 
AAGGATGACC CCAAGCTGAT GCAGGAGTGG TTCAAGTTGG TGCAAGAGAA GAATGCCATG 
GTGCGCTATG AGTCGGAGCT GATGATTTTT GCCCGGGAGC TGGAACTGGA GGACCGGCAG 
AGCCGACTGC AGCAGGAGCT CCGGGAGCGG ATGGCGGTGG AAGATCACCT GAAGACTGAG 
GAGGAGCTGT CTGAGGAGAA GAAGATCTTG AACGAGATGC TGGAGGTGGT GGAGCAGAGA 
GATGCCCTCG TGGCCCTGCT TGAGGAGCAG CGACTCCGGG AGAAGGAGGA GGACAAAGAC 
CTGGAGGCTG TCATGCTGTC CAAGGGCTTC AGCCTGAACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022266582.1
CDS	374..7201
Translation	MEDSKNETVNHAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDYRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKACTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFQGLVQPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELKEATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLNYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREQNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNININFLRPSQVRHLYNTGETRDIHLEMENLVNSRTTPKLTRNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGKEMASVGEPDKLSMVMYLTQFYEMFKDSLPSSDALDLNAEEKAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEEAPRGYRGGRPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSTGMRRQPTQERGVSQPSCCLPEQGHPAPTPQWKQQQEKERSRTCPKKVITLSPPLTPPPCRAPRSQQTYRDLDADSRGKQSPHHERPEPEPSRRFFVDQWELSLSLRSSNRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCRKRVYVMERLSAEGKFFHRSCFKCEYCATTLRLSAYAYDIEDGKFYCKPHYCYRLSGSAQRKRPAVAPLSGKEAREPLQDGPTVDTNGRASAVTSPAERTPGSSVNGVEEPSIAKRLRGTPERIELENYRLSVKQAEELEEVPEETQAEHNLSSVLDTGAEEDVASSSSESEMEEEEEEEPPLPTSDLGGVPWKEAVRIHALLKGKSEEELEASKSFGPGHEEDEEEEEYEEEEEEEEEEYEEEEEEEESSEEGEYCPWDRELLQGQWLRQLSQEETTGTRKVGSQRLQQIVNPADPLEIQADVHWTHIREREEEERMVPTSESSTSRVPDLPSIRRAAVQAWLETVSGVPFDENDLEEDVDSEPAKIEGEATENGDTGDTGAELDDDQRWSDGIPSDAETDLRVQHQEAVEADLELRVSENEEEPPEVTPGHPERSLTRVPSPALSPEEQAGLRSPGHSPKAEGAQIPLVSVVTKAKLPEENLFPKPLLPKVKPKVEVPCSQRAACSPIRSQPVALPEARTPTSPVSSQCLSPLATSTPTSTQLPICSQPQPSSETTAPSPTKSPIRLQPVSAKTSIPVVPLPLKNQGDTKDRLGSPLAVDEALKRNDLVAEFWMKSAEIRRSLGLTPVDRSKVYEPSFPSPAFKPVSLKSYAVEKSPEGEGLQLLKPPPVPKRLGLPKSDGDQPFLPTPKSPSDRELKSSQEERRDLSSSSGLGLHGSSSNMKTLGSQSFNTSDSTMLTPPSSPPPPPPQDEEPATLRRKPYQTHEHKDAEPKASVIPPPPPATFMRAPREPTQPLQEEMRKSFVESVDEIPFADDVEDTYDDKTEDSSLQEKFFTPPSYWPRTERPLHPPLAKENGRLPTLESGVQPQKRGLPLVSPEAKELAEERMRAREKSVKSQALRDAMAKQLSKMKEMEMAAGTSSPPGGTSHKASSVPSKGKDLSPESPRRPVLKGPREPALKHEATSEEVLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRNKKEKKSKGEGRPPEKPSPSLLEEAAAKPKSLWKSVFSGYKKDKKKKGDDKSCSSTPSSGATVDSGKHKMSPVVRAELHLRRQLSFSEDSDLSSDDILERSSQKSRRESVYVPHALAFKRSYPSKPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELSEEKKILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAVMLSKGFSLNWS

Target ORF information:

RefSeq Version	XM_022410874.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022410874.1

ORF Insert Sequence:

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTGCCTGAC CTTCCCTCCA TACGCCGCGC AGCAGTGCAG 
GCCTGGCTGG AGACGGTCTC TGGAGTCCCA TTTGATGAGA ATGACCTGGA GGAAGACGTG 
GACTCGGAGC CAGCAAAGAT AGAAGGGGAG GCCACAGAGA ATGGGGACAC AGGGGACACT 
GGTGCTGAGC TGGACGATGA TCAGCGCTGG TCTGACGGGA TCCCATCGGA TGCCGAGACG 
GATCTCCGCG TGCAGCACCA GGAGGCGGTA GAGGCAGACC TGGAGCTGAG GGTCTCAGAG 
AATGAGGAGG AGCCACCTGA GGTCACACCA GGGCATCCGG AGAGAAGTCT CACCCGGGTC 
CCCAGCCCTG CCCTGTCCCC TGAGGAGCAA GCTGGTCTGC GGTCCCCAGG CCACAGCCCT 
AAGGCAGAGG GGGCCCAAAT CCCACTTGTC TCTGTTGTCA CCAAGGCAAA ATTGCCTGAA 
GAGAACCTTT TCCCCAAGCC TTTGCTCCCC AAAGTGAAGC CCAAGGTGGA AGTGCCCTGC 
AGTCAGAGAG CTGCATGCTC CCCCATCCGT TCTCAGCCAG TGGCTCTGCC AGAAGCCAGG 
ACACCTACCT CCCCAGTGAG CTCGCAGTGC CTGTCGCCTT TGGCCACCTC CACCCCCACC 
AGCACCCAGC TCCCCATTTG CTCCCAGCCT CAGCCTTCCT CTGAGACCAC GGCCCCATCC 
CCCACCAAGT CCCCCATACG GCTCCAGCCT GTTTCAGCCA AAACATCTAT CCCAGTGGTT 
CCCCTCCCCT TGAAGAACCA AGGGGACACC AAGGACAGGC TGGGCAGCCC TCTCGCTGTG 
GATGAGGCCC TGAAGCGGAA TGACCTGGTG GCAGAGTTCT GGATGAAGAG CGCCGAGATC 
CGCCGCAGCC TCGGGCTCAC ACCAGTGGAT CGGAGCAAGG TGTATGAGCC CAGCTTCCCT 
TCGCCTGCCT TTAAACCAGT GTCCCTAAAA TCCTATGCAG TGGAGAAGTC TCCCGAGGGG 
GAGGGACTCC AGCTTCTGAA GCCTCCACCT GTTCCTAAGA GACTAGGCCT GCCGAAGTCA 
GACGGTGACC AGCCCTTCCT GCCAACCCCC AAGTCCCCGT CGGACAGAGA GCTGAAAAGC 
TCCCAGGAGG AGAGGAGAGA TCTGTCGAGC AGCTCCGGGC TGGGCCTTCA CGGGAGCTCC 
TCCAACATGA AGACTTTGGG CAGCCAGAGC TTCAACACAT CAGACTCCAC CATGCTCACT 
CCCCCTTCAA GCCCACCGCC ACCACCACCC CAAGACGAGG AACCCGCAAC TCTTCGGAGG 
AAGCCATATC AGACTCATGA GCATAAGGAC GCTGAGCCCA AGGCCTCGGT GATTCCACCA 
CCACCACCTG CGACGTTCAT GCGGGCCCCC CGGGAGCCCA CCCAGCCCCT CCAAGAGGAG 
ATGCGGAAGT CGTTTGTGGA GAGCGTGGAT GAGATCCCAT TTGCCGATGA TGTGGAGGAC 
ACCTATGATG ACAAGACCGA GGACTCGAGT CTCCAGGAGA AGTTCTTCAC ACCCCCCTCC 
TACTGGCCCC GCACGGAGAG GCCCCTCCAC CCACCCCTAG CCAAGGAGAA TGGTAGGTTG 
CCTACTCTGG AGAGTGGGGT CCAGCCACAG AAGAGGGGCC TGCCCTTAGT CTCTCCAGAA 
GCCAAGGAGC TGGCTGAGGA GCGCATGCGA GCCAGGGAGA AGTCTGTGAA GAGCCAGGCG 
TTGAGAGACG CTATGGCCAA GCAGCTGAGT AAGATGAAGG AAATGGAGAT GGCGGCTGGG 
ACCTCCAGTC CCCCAGGAGG CACCTCCCAC AAAGCCTCCT CTGTTCCCTC CAAGGGCAAA 
GACCTCAGCC CCGAGTCCCC TAGACGCCCA GTTCTCAAAG GCCCCAGGGA GCCTGCCCTG 
AAGCACGAAG CCACTAGTGA GGAGGTCCTC TCCCCACCAT CGGACTCAGG GGGCCCAGAT 
GGTTCGGTCA CCTCATCCGA GGGCTCCAGT GGGAAGAGCA AGAAGAGATC GTCACTCTTC 
TCCCCCCGCA GAAACAAGAA AGAGAAGAAG TCCAAAGGTG AGGGCCGGCC CCCGGAGAAG 
CCCAGCCCAA GCCTTCTGGA GGAAGCTGCT GCCAAGCCCA AGTCCCTGTG GAAGTCGGTC 
TTTTCCGGGT ACAAGAAGGA CAAAAAGAAG AAGGGTGATG ACAAGTCCTG TTCCAGCACC 
CCTTCCAGCG GTGCCACTGT GGACTCTGGC AAGCACAAGA TGTCCCCTGT TGTGAGAGCA 
GAGCTGCACC TGCGGCGCCA GTTGAGCTTC TCCGAGGACT CAGACCTGTC CAGTGATGAC 
ATCCTTGAGA GGTCCTCCCA GAAGTCCAGG CGAGAGTCGG TTTATGTACC ACACGCCTTG 
GCATTCAAGA GATCATATCC ATCAAAGCCG AGAACCTACA CAGAGGAGGA ACTGAATGCC 
AAGCTGACCC GGCGTGTGCA GAAGGCAGCT CGTAGACAGG CCAAGCAAGA GGAGCTGAAG 
CGGCTGCACC GAGCACAGAT CATCCAGAGG CAGCTGGAGC AGGTGGAGGA GAAGCAGAGA 
CAGCTGGAGG AAAGAGGGGT GGCCGTGGAG AAGGCGCTCC GTGGTGAAGC AGACTATTGG 
GGAGAGTCTT ATTACAGTGA CCTCATCGAC TTGCATCTGG GTGTTGAGCC CAGTGGCGGG 
ACTCCACGGC GTAGACCTCT CTCCTTCTGC CCTTGCTGTG TCCAGGAAGG CATGGGCAAG 
AAGGATGACC CCAAGCTGAT GCAGGAGTGG TTCAAGTTGG TGCAAGAGAA GAATGCCATG 
GTGCGCTATG AGTCGGAGCT GATGATTTTT GCCCGGGAGC TGGAACTGGA GGACCGGCAG 
AGCCGACTGC AGCAGGAGCT CCGGGAGCGG ATGGCGGTGG AAGATCACCT GAAGACTGAG 
GAGGAGCTGT CTGAGGAGAA GAAGATCTTG AACGAGATGC TGGAGGTGGT GGAGCAGAGA 
GATGCCCTCG TGGCCCTGCT TGAGGAGCAG CGACTCCGGG AGAAGGAGGA GGACAAAGAC 
CTGGAGGCTG TCATGCTGTC CAAGGGCTTC AGCCTGAACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_014108386.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963861.1 Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X4, mRNA.

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CloneID	OCe43613
Clone ID Related Accession (Same CDS sequence)	XM_014108387.2 , XM_014108388.2 , XM_014108383.1 , XM_014108384.1 , XM_014108383.2 , XM_022410874.1 , XM_014108386.1 , XM_014108386.2 , XM_022410875.1 , XM_014108385.1 , XM_014108387.1 , XM_014108388.1
Accession Version	XM_014108386.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTGCCTGAC CTTCCCTCCA TACGCCGCGC AGCAGTGCAG 
GCCTGGCTGG AGACGGTCTC TGGAGTCCCA TTTGATGAGA ATGACCTGGA GGAAGACGTG 
GACTCGGAGC CAGCAAAGAT AGAAGGGGAG GCCACAGAGA ATGGGGACAC AGGGGACACT 
GGTGCTGAGC TGGACGATGA TCAGCGCTGG TCTGACGGGA TCCCATCGGA TGCCGAGACG 
GATCTCCGCG TGCAGCACCA GGAGGCGGTA GAGGCAGACC TGGAGCTGAG GGTCTCAGAG 
AATGAGGAGG AGCCACCTGA GGTCACACCA GGGCATCCGG AGAGAAGTCT CACCCGGGTC 
CCCAGCCCTG CCCTGTCCCC TGAGGAGCAA GCTGGTCTGC GGTCCCCAGG CCACAGCCCT 
AAGGCAGAGG GGGCCCAAAT CCCACTTGTC TCTGTTGTCA CCAAGGCAAA ATTGCCTGAA 
GAGAACCTTT TCCCCAAGCC TTTGCTCCCC AAAGTGAAGC CCAAGGTGGA AGTGCCCTGC 
AGTCAGAGAG CTGCATGCTC CCCCATCCGT TCTCAGCCAG TGGCTCTGCC AGAAGCCAGG 
ACACCTACCT CCCCAGTGAG CTCGCAGTGC CTGTCGCCTT TGGCCACCTC CACCCCCACC 
AGCACCCAGC TCCCCATTTG CTCCCAGCCT CAGCCTTCCT CTGAGACCAC GGCCCCATCC 
CCCACCAAGT CCCCCATACG GCTCCAGCCT GTTTCAGCCA AAACATCTAT CCCAGTGGTT 
CCCCTCCCCT TGAAGAACCA AGGGGACACC AAGGACAGGC TGGGCAGCCC TCTCGCTGTG 
GATGAGGCCC TGAAGCGGAA TGACCTGGTG GCAGAGTTCT GGATGAAGAG CGCCGAGATC 
CGCCGCAGCC TCGGGCTCAC ACCAGTGGAT CGGAGCAAGG TGTATGAGCC CAGCTTCCCT 
TCGCCTGCCT TTAAACCAGT GTCCCTAAAA TCCTATGCAG TGGAGAAGTC TCCCGAGGGG 
GAGGGACTCC AGCTTCTGAA GCCTCCACCT GTTCCTAAGA GACTAGGCCT GCCGAAGTCA 
GACGGTGACC AGCCCTTCCT GCCAACCCCC AAGTCCCCGT CGGACAGAGA GCTGAAAAGC 
TCCCAGGAGG AGAGGAGAGA TCTGTCGAGC AGCTCCGGGC TGGGCCTTCA CGGGAGCTCC 
TCCAACATGA AGACTTTGGG CAGCCAGAGC TTCAACACAT CAGACTCCAC CATGCTCACT 
CCCCCTTCAA GCCCACCGCC ACCACCACCC CAAGACGAGG AACCCGCAAC TCTTCGGAGG 
AAGCCATATC AGACTCATGA GCATAAGGAC GCTGAGCCCA AGGCCTCGGT GATTCCACCA 
CCACCACCTG CGACGTTCAT GCGGGCCCCC CGGGAGCCCA CCCAGCCCCT CCAAGAGGAG 
ATGCGGAAGT CGTTTGTGGA GAGCGTGGAT GAGATCCCAT TTGCCGATGA TGTGGAGGAC 
ACCTATGATG ACAAGACCGA GGACTCGAGT CTCCAGGAGA AGTTCTTCAC ACCCCCCTCC 
TACTGGCCCC GCACGGAGAG GCCCCTCCAC CCACCCCTAG CCAAGGAGAA TGGTAGGTTG 
CCTACTCTGG AGAGTGGGGT CCAGCCACAG AAGAGGGGCC TGCCCTTAGT CTCTCCAGAA 
GCCAAGGAGC TGGCTGAGGA GCGCATGCGA GCCAGGGAGA AGTCTGTGAA GAGCCAGGCG 
TTGAGAGACG CTATGGCCAA GCAGCTGAGT AAGATGAAGG AAATGGAGAT GGCGGCTGGG 
ACCTCCAGTC CCCCAGGAGG CACCTCCCAC AAAGCCTCCT CTGTTCCCTC CAAGGGCAAA 
GACCTCAGCC CCGAGTCCCC TAGACGCCCA GTTCTCAAAG GCCCCAGGGA GCCTGCCCTG 
AAGCACGAAG CCACTAGTGA GGAGGTCCTC TCCCCACCAT CGGACTCAGG GGGCCCAGAT 
GGTTCGGTCA CCTCATCCGA GGGCTCCAGT GGGAAGAGCA AGAAGAGATC GTCACTCTTC 
TCCCCCCGCA GAAACAAGAA AGAGAAGAAG TCCAAAGGTG AGGGCCGGCC CCCGGAGAAG 
CCCAGCCCAA GCCTTCTGGA GGAAGCTGCT GCCAAGCCCA AGTCCCTGTG GAAGTCGGTC 
TTTTCCGGGT ACAAGAAGGA CAAAAAGAAG AAGGGTGATG ACAAGTCCTG TTCCAGCACC 
CCTTCCAGCG GTGCCACTGT GGACTCTGGC AAGCACAAGA TGTCCCCTGT TGTGAGAGCA 
GAGCTGCACC TGCGGCGCCA GTTGAGCTTC TCCGAGGACT CAGACCTGTC CAGTGATGAC 
ATCCTTGAGA GGTCCTCCCA GAAGTCCAGG CGAGAGTCGG TTTATGTACC ACACGCCTTG 
GCATTCAAGA GATCATATCC ATCAAAGCCG AGAACCTACA CAGAGGAGGA ACTGAATGCC 
AAGCTGACCC GGCGTGTGCA GAAGGCAGCT CGTAGACAGG CCAAGCAAGA GGAGCTGAAG 
CGGCTGCACC GAGCACAGAT CATCCAGAGG CAGCTGGAGC AGGTGGAGGA GAAGCAGAGA 
CAGCTGGAGG AAAGAGGGGT GGCCGTGGAG AAGGCGCTCC GTGGTGAAGC AGACTATTGG 
GGAGAGTCTT ATTACAGTGA CCTCATCGAC TTGCATCTGG GTGTTGAGCC CAGTGGCGGG 
ACTCCACGGC GTAGACCTCT CTCCTTCTGC CCTTGCTGTG TCCAGGAAGG CATGGGCAAG 
AAGGATGACC CCAAGCTGAT GCAGGAGTGG TTCAAGTTGG TGCAAGAGAA GAATGCCATG 
GTGCGCTATG AGTCGGAGCT GATGATTTTT GCCCGGGAGC TGGAACTGGA GGACCGGCAG 
AGCCGACTGC AGCAGGAGCT CCGGGAGCGG ATGGCGGTGG AAGATCACCT GAAGACTGAG 
GAGGAGCTGT CTGAGGAGAA GAAGATCTTG AACGAGATGC TGGAGGTGGT GGAGCAGAGA 
GATGCCCTCG TGGCCCTGCT TGAGGAGCAG CGACTCCGGG AGAAGGAGGA GGACAAAGAC 
CTGGAGGCTG TCATGCTGTC CAAGGGCTTC AGCCTGAACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963861.1
CDS	166..6993
Translation	MEDSKNETVNHAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDYRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKACTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFQGLVQPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELKEATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLNYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREQNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNININFLRPSQVRHLYNTGETRDIHLEMENLVNSRTTPKLTRNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGKEMASVGEPDKLSMVMYLTQFYEMFKDSLPSSDALDLNAEEKAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEEAPRGYRGGRPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSTGMRRQPTQERGVSQPSCCLPEQGHPAPTPQWKQQQEKERSRTCPKKVITLSPPLTPPPCRAPRSQQTYRDLDADSRGKQSPHHERPEPEPSRRFFVDQWELSLSLRSSNRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCRKRVYVMERLSAEGKFFHRSCFKCEYCATTLRLSAYAYDIEDGKFYCKPHYCYRLSGSAQRKRPAVAPLSGKEAREPLQDGPTVDTNGRASAVTSPAERTPGSSVNGVEEPSIAKRLRGTPERIELENYRLSVKQAEELEEVPEETQAEHNLSSVLDTGAEEDVASSSSESEMEEEEEEEPPLPTSDLGGVPWKEAVRIHALLKGKSEEELEASKSFGPGHEEDEEEEEYEEEEEEEEEEYEEEEEEEESSEEGEYCPWDRELLQGQWLRQLSQEETTGTRKVGSQRLQQIVNPADPLEIQADVHWTHIREREEEERMVPTSESSTSRVPDLPSIRRAAVQAWLETVSGVPFDENDLEEDVDSEPAKIEGEATENGDTGDTGAELDDDQRWSDGIPSDAETDLRVQHQEAVEADLELRVSENEEEPPEVTPGHPERSLTRVPSPALSPEEQAGLRSPGHSPKAEGAQIPLVSVVTKAKLPEENLFPKPLLPKVKPKVEVPCSQRAACSPIRSQPVALPEARTPTSPVSSQCLSPLATSTPTSTQLPICSQPQPSSETTAPSPTKSPIRLQPVSAKTSIPVVPLPLKNQGDTKDRLGSPLAVDEALKRNDLVAEFWMKSAEIRRSLGLTPVDRSKVYEPSFPSPAFKPVSLKSYAVEKSPEGEGLQLLKPPPVPKRLGLPKSDGDQPFLPTPKSPSDRELKSSQEERRDLSSSSGLGLHGSSSNMKTLGSQSFNTSDSTMLTPPSSPPPPPPQDEEPATLRRKPYQTHEHKDAEPKASVIPPPPPATFMRAPREPTQPLQEEMRKSFVESVDEIPFADDVEDTYDDKTEDSSLQEKFFTPPSYWPRTERPLHPPLAKENGRLPTLESGVQPQKRGLPLVSPEAKELAEERMRAREKSVKSQALRDAMAKQLSKMKEMEMAAGTSSPPGGTSHKASSVPSKGKDLSPESPRRPVLKGPREPALKHEATSEEVLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRNKKEKKSKGEGRPPEKPSPSLLEEAAAKPKSLWKSVFSGYKKDKKKKGDDKSCSSTPSSGATVDSGKHKMSPVVRAELHLRRQLSFSEDSDLSSDDILERSSQKSRRESVYVPHALAFKRSYPSKPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELSEEKKILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAVMLSKGFSLNWS

Target ORF information:

RefSeq Version	XM_014108386.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108386.1

ORF Insert Sequence:

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTGCCTGAC CTTCCCTCCA TACGCCGCGC AGCAGTGCAG 
GCCTGGCTGG AGACGGTCTC TGGAGTCCCA TTTGATGAGA ATGACCTGGA GGAAGACGTG 
GACTCGGAGC CAGCAAAGAT AGAAGGGGAG GCCACAGAGA ATGGGGACAC AGGGGACACT 
GGTGCTGAGC TGGACGATGA TCAGCGCTGG TCTGACGGGA TCCCATCGGA TGCCGAGACG 
GATCTCCGCG TGCAGCACCA GGAGGCGGTA GAGGCAGACC TGGAGCTGAG GGTCTCAGAG 
AATGAGGAGG AGCCACCTGA GGTCACACCA GGGCATCCGG AGAGAAGTCT CACCCGGGTC 
CCCAGCCCTG CCCTGTCCCC TGAGGAGCAA GCTGGTCTGC GGTCCCCAGG CCACAGCCCT 
AAGGCAGAGG GGGCCCAAAT CCCACTTGTC TCTGTTGTCA CCAAGGCAAA ATTGCCTGAA 
GAGAACCTTT TCCCCAAGCC TTTGCTCCCC AAAGTGAAGC CCAAGGTGGA AGTGCCCTGC 
AGTCAGAGAG CTGCATGCTC CCCCATCCGT TCTCAGCCAG TGGCTCTGCC AGAAGCCAGG 
ACACCTACCT CCCCAGTGAG CTCGCAGTGC CTGTCGCCTT TGGCCACCTC CACCCCCACC 
AGCACCCAGC TCCCCATTTG CTCCCAGCCT CAGCCTTCCT CTGAGACCAC GGCCCCATCC 
CCCACCAAGT CCCCCATACG GCTCCAGCCT GTTTCAGCCA AAACATCTAT CCCAGTGGTT 
CCCCTCCCCT TGAAGAACCA AGGGGACACC AAGGACAGGC TGGGCAGCCC TCTCGCTGTG 
GATGAGGCCC TGAAGCGGAA TGACCTGGTG GCAGAGTTCT GGATGAAGAG CGCCGAGATC 
CGCCGCAGCC TCGGGCTCAC ACCAGTGGAT CGGAGCAAGG TGTATGAGCC CAGCTTCCCT 
TCGCCTGCCT TTAAACCAGT GTCCCTAAAA TCCTATGCAG TGGAGAAGTC TCCCGAGGGG 
GAGGGACTCC AGCTTCTGAA GCCTCCACCT GTTCCTAAGA GACTAGGCCT GCCGAAGTCA 
GACGGTGACC AGCCCTTCCT GCCAACCCCC AAGTCCCCGT CGGACAGAGA GCTGAAAAGC 
TCCCAGGAGG AGAGGAGAGA TCTGTCGAGC AGCTCCGGGC TGGGCCTTCA CGGGAGCTCC 
TCCAACATGA AGACTTTGGG CAGCCAGAGC TTCAACACAT CAGACTCCAC CATGCTCACT 
CCCCCTTCAA GCCCACCGCC ACCACCACCC CAAGACGAGG AACCCGCAAC TCTTCGGAGG 
AAGCCATATC AGACTCATGA GCATAAGGAC GCTGAGCCCA AGGCCTCGGT GATTCCACCA 
CCACCACCTG CGACGTTCAT GCGGGCCCCC CGGGAGCCCA CCCAGCCCCT CCAAGAGGAG 
ATGCGGAAGT CGTTTGTGGA GAGCGTGGAT GAGATCCCAT TTGCCGATGA TGTGGAGGAC 
ACCTATGATG ACAAGACCGA GGACTCGAGT CTCCAGGAGA AGTTCTTCAC ACCCCCCTCC 
TACTGGCCCC GCACGGAGAG GCCCCTCCAC CCACCCCTAG CCAAGGAGAA TGGTAGGTTG 
CCTACTCTGG AGAGTGGGGT CCAGCCACAG AAGAGGGGCC TGCCCTTAGT CTCTCCAGAA 
GCCAAGGAGC TGGCTGAGGA GCGCATGCGA GCCAGGGAGA AGTCTGTGAA GAGCCAGGCG 
TTGAGAGACG CTATGGCCAA GCAGCTGAGT AAGATGAAGG AAATGGAGAT GGCGGCTGGG 
ACCTCCAGTC CCCCAGGAGG CACCTCCCAC AAAGCCTCCT CTGTTCCCTC CAAGGGCAAA 
GACCTCAGCC CCGAGTCCCC TAGACGCCCA GTTCTCAAAG GCCCCAGGGA GCCTGCCCTG 
AAGCACGAAG CCACTAGTGA GGAGGTCCTC TCCCCACCAT CGGACTCAGG GGGCCCAGAT 
GGTTCGGTCA CCTCATCCGA GGGCTCCAGT GGGAAGAGCA AGAAGAGATC GTCACTCTTC 
TCCCCCCGCA GAAACAAGAA AGAGAAGAAG TCCAAAGGTG AGGGCCGGCC CCCGGAGAAG 
CCCAGCCCAA GCCTTCTGGA GGAAGCTGCT GCCAAGCCCA AGTCCCTGTG GAAGTCGGTC 
TTTTCCGGGT ACAAGAAGGA CAAAAAGAAG AAGGGTGATG ACAAGTCCTG TTCCAGCACC 
CCTTCCAGCG GTGCCACTGT GGACTCTGGC AAGCACAAGA TGTCCCCTGT TGTGAGAGCA 
GAGCTGCACC TGCGGCGCCA GTTGAGCTTC TCCGAGGACT CAGACCTGTC CAGTGATGAC 
ATCCTTGAGA GGTCCTCCCA GAAGTCCAGG CGAGAGTCGG TTTATGTACC ACACGCCTTG 
GCATTCAAGA GATCATATCC ATCAAAGCCG AGAACCTACA CAGAGGAGGA ACTGAATGCC 
AAGCTGACCC GGCGTGTGCA GAAGGCAGCT CGTAGACAGG CCAAGCAAGA GGAGCTGAAG 
CGGCTGCACC GAGCACAGAT CATCCAGAGG CAGCTGGAGC AGGTGGAGGA GAAGCAGAGA 
CAGCTGGAGG AAAGAGGGGT GGCCGTGGAG AAGGCGCTCC GTGGTGAAGC AGACTATTGG 
GGAGAGTCTT ATTACAGTGA CCTCATCGAC TTGCATCTGG GTGTTGAGCC CAGTGGCGGG 
ACTCCACGGC GTAGACCTCT CTCCTTCTGC CCTTGCTGTG TCCAGGAAGG CATGGGCAAG 
AAGGATGACC CCAAGCTGAT GCAGGAGTGG TTCAAGTTGG TGCAAGAGAA GAATGCCATG 
GTGCGCTATG AGTCGGAGCT GATGATTTTT GCCCGGGAGC TGGAACTGGA GGACCGGCAG 
AGCCGACTGC AGCAGGAGCT CCGGGAGCGG ATGGCGGTGG AAGATCACCT GAAGACTGAG 
GAGGAGCTGT CTGAGGAGAA GAAGATCTTG AACGAGATGC TGGAGGTGGT GGAGCAGAGA 
GATGCCCTCG TGGCCCTGCT TGAGGAGCAG CGACTCCGGG AGAAGGAGGA GGACAAAGAC 
CTGGAGGCTG TCATGCTGTC CAAGGGCTTC AGCCTGAACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_014108386.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963861.1 Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X3, mRNA.

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CloneID	OCe43613
Clone ID Related Accession (Same CDS sequence)	XM_014108387.2 , XM_014108388.2 , XM_014108383.1 , XM_014108384.1 , XM_014108383.2 , XM_022410874.1 , XM_014108386.1 , XM_014108386.2 , XM_022410875.1 , XM_014108385.1 , XM_014108387.1 , XM_014108388.1
Accession Version	XM_014108386.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	F-actin-methionine sulfoxide oxidase MICAL3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014108386.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTGCCTGAC CTTCCCTCCA TACGCCGCGC AGCAGTGCAG 
GCCTGGCTGG AGACGGTCTC TGGAGTCCCA TTTGATGAGA ATGACCTGGA GGAAGACGTG 
GACTCGGAGC CAGCAAAGAT AGAAGGGGAG GCCACAGAGA ATGGGGACAC AGGGGACACT 
GGTGCTGAGC TGGACGATGA TCAGCGCTGG TCTGACGGGA TCCCATCGGA TGCCGAGACG 
GATCTCCGCG TGCAGCACCA GGAGGCGGTA GAGGCAGACC TGGAGCTGAG GGTCTCAGAG 
AATGAGGAGG AGCCACCTGA GGTCACACCA GGGCATCCGG AGAGAAGTCT CACCCGGGTC 
CCCAGCCCTG CCCTGTCCCC TGAGGAGCAA GCTGGTCTGC GGTCCCCAGG CCACAGCCCT 
AAGGCAGAGG GGGCCCAAAT CCCACTTGTC TCTGTTGTCA CCAAGGCAAA ATTGCCTGAA 
GAGAACCTTT TCCCCAAGCC TTTGCTCCCC AAAGTGAAGC CCAAGGTGGA AGTGCCCTGC 
AGTCAGAGAG CTGCATGCTC CCCCATCCGT TCTCAGCCAG TGGCTCTGCC AGAAGCCAGG 
ACACCTACCT CCCCAGTGAG CTCGCAGTGC CTGTCGCCTT TGGCCACCTC CACCCCCACC 
AGCACCCAGC TCCCCATTTG CTCCCAGCCT CAGCCTTCCT CTGAGACCAC GGCCCCATCC 
CCCACCAAGT CCCCCATACG GCTCCAGCCT GTTTCAGCCA AAACATCTAT CCCAGTGGTT 
CCCCTCCCCT TGAAGAACCA AGGGGACACC AAGGACAGGC TGGGCAGCCC TCTCGCTGTG 
GATGAGGCCC TGAAGCGGAA TGACCTGGTG GCAGAGTTCT GGATGAAGAG CGCCGAGATC 
CGCCGCAGCC TCGGGCTCAC ACCAGTGGAT CGGAGCAAGG TGTATGAGCC CAGCTTCCCT 
TCGCCTGCCT TTAAACCAGT GTCCCTAAAA TCCTATGCAG TGGAGAAGTC TCCCGAGGGG 
GAGGGACTCC AGCTTCTGAA GCCTCCACCT GTTCCTAAGA GACTAGGCCT GCCGAAGTCA 
GACGGTGACC AGCCCTTCCT GCCAACCCCC AAGTCCCCGT CGGACAGAGA GCTGAAAAGC 
TCCCAGGAGG AGAGGAGAGA TCTGTCGAGC AGCTCCGGGC TGGGCCTTCA CGGGAGCTCC 
TCCAACATGA AGACTTTGGG CAGCCAGAGC TTCAACACAT CAGACTCCAC CATGCTCACT 
CCCCCTTCAA GCCCACCGCC ACCACCACCC CAAGACGAGG AACCCGCAAC TCTTCGGAGG 
AAGCCATATC AGACTCATGA GCATAAGGAC GCTGAGCCCA AGGCCTCGGT GATTCCACCA 
CCACCACCTG CGACGTTCAT GCGGGCCCCC CGGGAGCCCA CCCAGCCCCT CCAAGAGGAG 
ATGCGGAAGT CGTTTGTGGA GAGCGTGGAT GAGATCCCAT TTGCCGATGA TGTGGAGGAC 
ACCTATGATG ACAAGACCGA GGACTCGAGT CTCCAGGAGA AGTTCTTCAC ACCCCCCTCC 
TACTGGCCCC GCACGGAGAG GCCCCTCCAC CCACCCCTAG CCAAGGAGAA TGGTAGGTTG 
CCTACTCTGG AGAGTGGGGT CCAGCCACAG AAGAGGGGCC TGCCCTTAGT CTCTCCAGAA 
GCCAAGGAGC TGGCTGAGGA GCGCATGCGA GCCAGGGAGA AGTCTGTGAA GAGCCAGGCG 
TTGAGAGACG CTATGGCCAA GCAGCTGAGT AAGATGAAGG AAATGGAGAT GGCGGCTGGG 
ACCTCCAGTC CCCCAGGAGG CACCTCCCAC AAAGCCTCCT CTGTTCCCTC CAAGGGCAAA 
GACCTCAGCC CCGAGTCCCC TAGACGCCCA GTTCTCAAAG GCCCCAGGGA GCCTGCCCTG 
AAGCACGAAG CCACTAGTGA GGAGGTCCTC TCCCCACCAT CGGACTCAGG GGGCCCAGAT 
GGTTCGGTCA CCTCATCCGA GGGCTCCAGT GGGAAGAGCA AGAAGAGATC GTCACTCTTC 
TCCCCCCGCA GAAACAAGAA AGAGAAGAAG TCCAAAGGTG AGGGCCGGCC CCCGGAGAAG 
CCCAGCCCAA GCCTTCTGGA GGAAGCTGCT GCCAAGCCCA AGTCCCTGTG GAAGTCGGTC 
TTTTCCGGGT ACAAGAAGGA CAAAAAGAAG AAGGGTGATG ACAAGTCCTG TTCCAGCACC 
CCTTCCAGCG GTGCCACTGT GGACTCTGGC AAGCACAAGA TGTCCCCTGT TGTGAGAGCA 
GAGCTGCACC TGCGGCGCCA GTTGAGCTTC TCCGAGGACT CAGACCTGTC CAGTGATGAC 
ATCCTTGAGA GGTCCTCCCA GAAGTCCAGG CGAGAGTCGG TTTATGTACC ACACGCCTTG 
GCATTCAAGA GATCATATCC ATCAAAGCCG AGAACCTACA CAGAGGAGGA ACTGAATGCC 
AAGCTGACCC GGCGTGTGCA GAAGGCAGCT CGTAGACAGG CCAAGCAAGA GGAGCTGAAG 
CGGCTGCACC GAGCACAGAT CATCCAGAGG CAGCTGGAGC AGGTGGAGGA GAAGCAGAGA 
CAGCTGGAGG AAAGAGGGGT GGCCGTGGAG AAGGCGCTCC GTGGTGAAGC AGACTATTGG 
GGAGAGTCTT ATTACAGTGA CCTCATCGAC TTGCATCTGG GTGTTGAGCC CAGTGGCGGG 
ACTCCACGGC GTAGACCTCT CTCCTTCTGC CCTTGCTGTG TCCAGGAAGG CATGGGCAAG 
AAGGATGACC CCAAGCTGAT GCAGGAGTGG TTCAAGTTGG TGCAAGAGAA GAATGCCATG 
GTGCGCTATG AGTCGGAGCT GATGATTTTT GCCCGGGAGC TGGAACTGGA GGACCGGCAG 
AGCCGACTGC AGCAGGAGCT CCGGGAGCGG ATGGCGGTGG AAGATCACCT GAAGACTGAG 
GAGGAGCTGT CTGAGGAGAA GAAGATCTTG AACGAGATGC TGGAGGTGGT GGAGCAGAGA 
GATGCCCTCG TGGCCCTGCT TGAGGAGCAG CGACTCCGGG AGAAGGAGGA GGACAAAGAC 
CTGGAGGCTG TCATGCTGTC CAAGGGCTTC AGCCTGAACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963861.1
CDS	195..7022
Translation	MEDSKNETVNHAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDYRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKACTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFQGLVQPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELKEATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLNYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREQNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNININFLRPSQVRHLYNTGETRDIHLEMENLVNSRTTPKLTRNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGKEMASVGEPDKLSMVMYLTQFYEMFKDSLPSSDALDLNAEEKAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEEAPRGYRGGRPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSTGMRRQPTQERGVSQPSCCLPEQGHPAPTPQWKQQQEKERSRTCPKKVITLSPPLTPPPCRAPRSQQTYRDLDADSRGKQSPHHERPEPEPSRRFFVDQWELSLSLRSSNRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCRKRVYVMERLSAEGKFFHRSCFKCEYCATTLRLSAYAYDIEDGKFYCKPHYCYRLSGSAQRKRPAVAPLSGKEAREPLQDGPTVDTNGRASAVTSPAERTPGSSVNGVEEPSIAKRLRGTPERIELENYRLSVKQAEELEEVPEETQAEHNLSSVLDTGAEEDVASSSSESEMEEEEEEEPPLPTSDLGGVPWKEAVRIHALLKGKSEEELEASKSFGPGHEEDEEEEEYEEEEEEEEEEYEEEEEEEESSEEGEYCPWDRELLQGQWLRQLSQEETTGTRKVGSQRLQQIVNPADPLEIQADVHWTHIREREEEERMVPTSESSTSRVPDLPSIRRAAVQAWLETVSGVPFDENDLEEDVDSEPAKIEGEATENGDTGDTGAELDDDQRWSDGIPSDAETDLRVQHQEAVEADLELRVSENEEEPPEVTPGHPERSLTRVPSPALSPEEQAGLRSPGHSPKAEGAQIPLVSVVTKAKLPEENLFPKPLLPKVKPKVEVPCSQRAACSPIRSQPVALPEARTPTSPVSSQCLSPLATSTPTSTQLPICSQPQPSSETTAPSPTKSPIRLQPVSAKTSIPVVPLPLKNQGDTKDRLGSPLAVDEALKRNDLVAEFWMKSAEIRRSLGLTPVDRSKVYEPSFPSPAFKPVSLKSYAVEKSPEGEGLQLLKPPPVPKRLGLPKSDGDQPFLPTPKSPSDRELKSSQEERRDLSSSSGLGLHGSSSNMKTLGSQSFNTSDSTMLTPPSSPPPPPPQDEEPATLRRKPYQTHEHKDAEPKASVIPPPPPATFMRAPREPTQPLQEEMRKSFVESVDEIPFADDVEDTYDDKTEDSSLQEKFFTPPSYWPRTERPLHPPLAKENGRLPTLESGVQPQKRGLPLVSPEAKELAEERMRAREKSVKSQALRDAMAKQLSKMKEMEMAAGTSSPPGGTSHKASSVPSKGKDLSPESPRRPVLKGPREPALKHEATSEEVLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRNKKEKKSKGEGRPPEKPSPSLLEEAAAKPKSLWKSVFSGYKKDKKKKGDDKSCSSTPSSGATVDSGKHKMSPVVRAELHLRRQLSFSEDSDLSSDDILERSSQKSRRESVYVPHALAFKRSYPSKPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELSEEKKILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAVMLSKGFSLNWS

Target ORF information:

RefSeq Version	XM_014108386.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108386.2

ORF Insert Sequence:

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTGCCTGAC CTTCCCTCCA TACGCCGCGC AGCAGTGCAG 
GCCTGGCTGG AGACGGTCTC TGGAGTCCCA TTTGATGAGA ATGACCTGGA GGAAGACGTG 
GACTCGGAGC CAGCAAAGAT AGAAGGGGAG GCCACAGAGA ATGGGGACAC AGGGGACACT 
GGTGCTGAGC TGGACGATGA TCAGCGCTGG TCTGACGGGA TCCCATCGGA TGCCGAGACG 
GATCTCCGCG TGCAGCACCA GGAGGCGGTA GAGGCAGACC TGGAGCTGAG GGTCTCAGAG 
AATGAGGAGG AGCCACCTGA GGTCACACCA GGGCATCCGG AGAGAAGTCT CACCCGGGTC 
CCCAGCCCTG CCCTGTCCCC TGAGGAGCAA GCTGGTCTGC GGTCCCCAGG CCACAGCCCT 
AAGGCAGAGG GGGCCCAAAT CCCACTTGTC TCTGTTGTCA CCAAGGCAAA ATTGCCTGAA 
GAGAACCTTT TCCCCAAGCC TTTGCTCCCC AAAGTGAAGC CCAAGGTGGA AGTGCCCTGC 
AGTCAGAGAG CTGCATGCTC CCCCATCCGT TCTCAGCCAG TGGCTCTGCC AGAAGCCAGG 
ACACCTACCT CCCCAGTGAG CTCGCAGTGC CTGTCGCCTT TGGCCACCTC CACCCCCACC 
AGCACCCAGC TCCCCATTTG CTCCCAGCCT CAGCCTTCCT CTGAGACCAC GGCCCCATCC 
CCCACCAAGT CCCCCATACG GCTCCAGCCT GTTTCAGCCA AAACATCTAT CCCAGTGGTT 
CCCCTCCCCT TGAAGAACCA AGGGGACACC AAGGACAGGC TGGGCAGCCC TCTCGCTGTG 
GATGAGGCCC TGAAGCGGAA TGACCTGGTG GCAGAGTTCT GGATGAAGAG CGCCGAGATC 
CGCCGCAGCC TCGGGCTCAC ACCAGTGGAT CGGAGCAAGG TGTATGAGCC CAGCTTCCCT 
TCGCCTGCCT TTAAACCAGT GTCCCTAAAA TCCTATGCAG TGGAGAAGTC TCCCGAGGGG 
GAGGGACTCC AGCTTCTGAA GCCTCCACCT GTTCCTAAGA GACTAGGCCT GCCGAAGTCA 
GACGGTGACC AGCCCTTCCT GCCAACCCCC AAGTCCCCGT CGGACAGAGA GCTGAAAAGC 
TCCCAGGAGG AGAGGAGAGA TCTGTCGAGC AGCTCCGGGC TGGGCCTTCA CGGGAGCTCC 
TCCAACATGA AGACTTTGGG CAGCCAGAGC TTCAACACAT CAGACTCCAC CATGCTCACT 
CCCCCTTCAA GCCCACCGCC ACCACCACCC CAAGACGAGG AACCCGCAAC TCTTCGGAGG 
AAGCCATATC AGACTCATGA GCATAAGGAC GCTGAGCCCA AGGCCTCGGT GATTCCACCA 
CCACCACCTG CGACGTTCAT GCGGGCCCCC CGGGAGCCCA CCCAGCCCCT CCAAGAGGAG 
ATGCGGAAGT CGTTTGTGGA GAGCGTGGAT GAGATCCCAT TTGCCGATGA TGTGGAGGAC 
ACCTATGATG ACAAGACCGA GGACTCGAGT CTCCAGGAGA AGTTCTTCAC ACCCCCCTCC 
TACTGGCCCC GCACGGAGAG GCCCCTCCAC CCACCCCTAG CCAAGGAGAA TGGTAGGTTG 
CCTACTCTGG AGAGTGGGGT CCAGCCACAG AAGAGGGGCC TGCCCTTAGT CTCTCCAGAA 
GCCAAGGAGC TGGCTGAGGA GCGCATGCGA GCCAGGGAGA AGTCTGTGAA GAGCCAGGCG 
TTGAGAGACG CTATGGCCAA GCAGCTGAGT AAGATGAAGG AAATGGAGAT GGCGGCTGGG 
ACCTCCAGTC CCCCAGGAGG CACCTCCCAC AAAGCCTCCT CTGTTCCCTC CAAGGGCAAA 
GACCTCAGCC CCGAGTCCCC TAGACGCCCA GTTCTCAAAG GCCCCAGGGA GCCTGCCCTG 
AAGCACGAAG CCACTAGTGA GGAGGTCCTC TCCCCACCAT CGGACTCAGG GGGCCCAGAT 
GGTTCGGTCA CCTCATCCGA GGGCTCCAGT GGGAAGAGCA AGAAGAGATC GTCACTCTTC 
TCCCCCCGCA GAAACAAGAA AGAGAAGAAG TCCAAAGGTG AGGGCCGGCC CCCGGAGAAG 
CCCAGCCCAA GCCTTCTGGA GGAAGCTGCT GCCAAGCCCA AGTCCCTGTG GAAGTCGGTC 
TTTTCCGGGT ACAAGAAGGA CAAAAAGAAG AAGGGTGATG ACAAGTCCTG TTCCAGCACC 
CCTTCCAGCG GTGCCACTGT GGACTCTGGC AAGCACAAGA TGTCCCCTGT TGTGAGAGCA 
GAGCTGCACC TGCGGCGCCA GTTGAGCTTC TCCGAGGACT CAGACCTGTC CAGTGATGAC 
ATCCTTGAGA GGTCCTCCCA GAAGTCCAGG CGAGAGTCGG TTTATGTACC ACACGCCTTG 
GCATTCAAGA GATCATATCC ATCAAAGCCG AGAACCTACA CAGAGGAGGA ACTGAATGCC 
AAGCTGACCC GGCGTGTGCA GAAGGCAGCT CGTAGACAGG CCAAGCAAGA GGAGCTGAAG 
CGGCTGCACC GAGCACAGAT CATCCAGAGG CAGCTGGAGC AGGTGGAGGA GAAGCAGAGA 
CAGCTGGAGG AAAGAGGGGT GGCCGTGGAG AAGGCGCTCC GTGGTGAAGC AGACTATTGG 
GGAGAGTCTT ATTACAGTGA CCTCATCGAC TTGCATCTGG GTGTTGAGCC CAGTGGCGGG 
ACTCCACGGC GTAGACCTCT CTCCTTCTGC CCTTGCTGTG TCCAGGAAGG CATGGGCAAG 
AAGGATGACC CCAAGCTGAT GCAGGAGTGG TTCAAGTTGG TGCAAGAGAA GAATGCCATG 
GTGCGCTATG AGTCGGAGCT GATGATTTTT GCCCGGGAGC TGGAACTGGA GGACCGGCAG 
AGCCGACTGC AGCAGGAGCT CCGGGAGCGG ATGGCGGTGG AAGATCACCT GAAGACTGAG 
GAGGAGCTGT CTGAGGAGAA GAAGATCTTG AACGAGATGC TGGAGGTGGT GGAGCAGAGA 
GATGCCCTCG TGGCCCTGCT TGAGGAGCAG CGACTCCGGG AGAAGGAGGA GGACAAAGAC 
CTGGAGGCTG TCATGCTGTC CAAGGGCTTC AGCCTGAACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_022410875.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022266583.1 Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X4, mRNA.

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CloneID	OCe43613
Clone ID Related Accession (Same CDS sequence)	XM_014108387.2 , XM_014108388.2 , XM_014108383.1 , XM_014108384.1 , XM_014108383.2 , XM_022410874.1 , XM_014108386.1 , XM_014108386.2 , XM_022410875.1 , XM_014108385.1 , XM_014108387.1 , XM_014108388.1
Accession Version	XM_022410875.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	F-actin-methionine sulfoxide oxidase MICAL3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTGCCTGAC CTTCCCTCCA TACGCCGCGC AGCAGTGCAG 
GCCTGGCTGG AGACGGTCTC TGGAGTCCCA TTTGATGAGA ATGACCTGGA GGAAGACGTG 
GACTCGGAGC CAGCAAAGAT AGAAGGGGAG GCCACAGAGA ATGGGGACAC AGGGGACACT 
GGTGCTGAGC TGGACGATGA TCAGCGCTGG TCTGACGGGA TCCCATCGGA TGCCGAGACG 
GATCTCCGCG TGCAGCACCA GGAGGCGGTA GAGGCAGACC TGGAGCTGAG GGTCTCAGAG 
AATGAGGAGG AGCCACCTGA GGTCACACCA GGGCATCCGG AGAGAAGTCT CACCCGGGTC 
CCCAGCCCTG CCCTGTCCCC TGAGGAGCAA GCTGGTCTGC GGTCCCCAGG CCACAGCCCT 
AAGGCAGAGG GGGCCCAAAT CCCACTTGTC TCTGTTGTCA CCAAGGCAAA ATTGCCTGAA 
GAGAACCTTT TCCCCAAGCC TTTGCTCCCC AAAGTGAAGC CCAAGGTGGA AGTGCCCTGC 
AGTCAGAGAG CTGCATGCTC CCCCATCCGT TCTCAGCCAG TGGCTCTGCC AGAAGCCAGG 
ACACCTACCT CCCCAGTGAG CTCGCAGTGC CTGTCGCCTT TGGCCACCTC CACCCCCACC 
AGCACCCAGC TCCCCATTTG CTCCCAGCCT CAGCCTTCCT CTGAGACCAC GGCCCCATCC 
CCCACCAAGT CCCCCATACG GCTCCAGCCT GTTTCAGCCA AAACATCTAT CCCAGTGGTT 
CCCCTCCCCT TGAAGAACCA AGGGGACACC AAGGACAGGC TGGGCAGCCC TCTCGCTGTG 
GATGAGGCCC TGAAGCGGAA TGACCTGGTG GCAGAGTTCT GGATGAAGAG CGCCGAGATC 
CGCCGCAGCC TCGGGCTCAC ACCAGTGGAT CGGAGCAAGG TGTATGAGCC CAGCTTCCCT 
TCGCCTGCCT TTAAACCAGT GTCCCTAAAA TCCTATGCAG TGGAGAAGTC TCCCGAGGGG 
GAGGGACTCC AGCTTCTGAA GCCTCCACCT GTTCCTAAGA GACTAGGCCT GCCGAAGTCA 
GACGGTGACC AGCCCTTCCT GCCAACCCCC AAGTCCCCGT CGGACAGAGA GCTGAAAAGC 
TCCCAGGAGG AGAGGAGAGA TCTGTCGAGC AGCTCCGGGC TGGGCCTTCA CGGGAGCTCC 
TCCAACATGA AGACTTTGGG CAGCCAGAGC TTCAACACAT CAGACTCCAC CATGCTCACT 
CCCCCTTCAA GCCCACCGCC ACCACCACCC CAAGACGAGG AACCCGCAAC TCTTCGGAGG 
AAGCCATATC AGACTCATGA GCATAAGGAC GCTGAGCCCA AGGCCTCGGT GATTCCACCA 
CCACCACCTG CGACGTTCAT GCGGGCCCCC CGGGAGCCCA CCCAGCCCCT CCAAGAGGAG 
ATGCGGAAGT CGTTTGTGGA GAGCGTGGAT GAGATCCCAT TTGCCGATGA TGTGGAGGAC 
ACCTATGATG ACAAGACCGA GGACTCGAGT CTCCAGGAGA AGTTCTTCAC ACCCCCCTCC 
TACTGGCCCC GCACGGAGAG GCCCCTCCAC CCACCCCTAG CCAAGGAGAA TGGTAGGTTG 
CCTACTCTGG AGAGTGGGGT CCAGCCACAG AAGAGGGGCC TGCCCTTAGT CTCTCCAGAA 
GCCAAGGAGC TGGCTGAGGA GCGCATGCGA GCCAGGGAGA AGTCTGTGAA GAGCCAGGCG 
TTGAGAGACG CTATGGCCAA GCAGCTGAGT AAGATGAAGG AAATGGAGAT GGCGGCTGGG 
ACCTCCAGTC CCCCAGGAGG CACCTCCCAC AAAGCCTCCT CTGTTCCCTC CAAGGGCAAA 
GACCTCAGCC CCGAGTCCCC TAGACGCCCA GTTCTCAAAG GCCCCAGGGA GCCTGCCCTG 
AAGCACGAAG CCACTAGTGA GGAGGTCCTC TCCCCACCAT CGGACTCAGG GGGCCCAGAT 
GGTTCGGTCA CCTCATCCGA GGGCTCCAGT GGGAAGAGCA AGAAGAGATC GTCACTCTTC 
TCCCCCCGCA GAAACAAGAA AGAGAAGAAG TCCAAAGGTG AGGGCCGGCC CCCGGAGAAG 
CCCAGCCCAA GCCTTCTGGA GGAAGCTGCT GCCAAGCCCA AGTCCCTGTG GAAGTCGGTC 
TTTTCCGGGT ACAAGAAGGA CAAAAAGAAG AAGGGTGATG ACAAGTCCTG TTCCAGCACC 
CCTTCCAGCG GTGCCACTGT GGACTCTGGC AAGCACAAGA TGTCCCCTGT TGTGAGAGCA 
GAGCTGCACC TGCGGCGCCA GTTGAGCTTC TCCGAGGACT CAGACCTGTC CAGTGATGAC 
ATCCTTGAGA GGTCCTCCCA GAAGTCCAGG CGAGAGTCGG TTTATGTACC ACACGCCTTG 
GCATTCAAGA GATCATATCC ATCAAAGCCG AGAACCTACA CAGAGGAGGA ACTGAATGCC 
AAGCTGACCC GGCGTGTGCA GAAGGCAGCT CGTAGACAGG CCAAGCAAGA GGAGCTGAAG 
CGGCTGCACC GAGCACAGAT CATCCAGAGG CAGCTGGAGC AGGTGGAGGA GAAGCAGAGA 
CAGCTGGAGG AAAGAGGGGT GGCCGTGGAG AAGGCGCTCC GTGGTGAAGC AGACTATTGG 
GGAGAGTCTT ATTACAGTGA CCTCATCGAC TTGCATCTGG GTGTTGAGCC CAGTGGCGGG 
ACTCCACGGC GTAGACCTCT CTCCTTCTGC CCTTGCTGTG TCCAGGAAGG CATGGGCAAG 
AAGGATGACC CCAAGCTGAT GCAGGAGTGG TTCAAGTTGG TGCAAGAGAA GAATGCCATG 
GTGCGCTATG AGTCGGAGCT GATGATTTTT GCCCGGGAGC TGGAACTGGA GGACCGGCAG 
AGCCGACTGC AGCAGGAGCT CCGGGAGCGG ATGGCGGTGG AAGATCACCT GAAGACTGAG 
GAGGAGCTGT CTGAGGAGAA GAAGATCTTG AACGAGATGC TGGAGGTGGT GGAGCAGAGA 
GATGCCCTCG TGGCCCTGCT TGAGGAGCAG CGACTCCGGG AGAAGGAGGA GGACAAAGAC 
CTGGAGGCTG TCATGCTGTC CAAGGGCTTC AGCCTGAACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022266583.1
CDS	654..7481
Translation	MEDSKNETVNHAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDYRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKACTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFQGLVQPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELKEATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLNYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREQNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNININFLRPSQVRHLYNTGETRDIHLEMENLVNSRTTPKLTRNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGKEMASVGEPDKLSMVMYLTQFYEMFKDSLPSSDALDLNAEEKAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEEAPRGYRGGRPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSTGMRRQPTQERGVSQPSCCLPEQGHPAPTPQWKQQQEKERSRTCPKKVITLSPPLTPPPCRAPRSQQTYRDLDADSRGKQSPHHERPEPEPSRRFFVDQWELSLSLRSSNRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCRKRVYVMERLSAEGKFFHRSCFKCEYCATTLRLSAYAYDIEDGKFYCKPHYCYRLSGSAQRKRPAVAPLSGKEAREPLQDGPTVDTNGRASAVTSPAERTPGSSVNGVEEPSIAKRLRGTPERIELENYRLSVKQAEELEEVPEETQAEHNLSSVLDTGAEEDVASSSSESEMEEEEEEEPPLPTSDLGGVPWKEAVRIHALLKGKSEEELEASKSFGPGHEEDEEEEEYEEEEEEEEEEYEEEEEEEESSEEGEYCPWDRELLQGQWLRQLSQEETTGTRKVGSQRLQQIVNPADPLEIQADVHWTHIREREEEERMVPTSESSTSRVPDLPSIRRAAVQAWLETVSGVPFDENDLEEDVDSEPAKIEGEATENGDTGDTGAELDDDQRWSDGIPSDAETDLRVQHQEAVEADLELRVSENEEEPPEVTPGHPERSLTRVPSPALSPEEQAGLRSPGHSPKAEGAQIPLVSVVTKAKLPEENLFPKPLLPKVKPKVEVPCSQRAACSPIRSQPVALPEARTPTSPVSSQCLSPLATSTPTSTQLPICSQPQPSSETTAPSPTKSPIRLQPVSAKTSIPVVPLPLKNQGDTKDRLGSPLAVDEALKRNDLVAEFWMKSAEIRRSLGLTPVDRSKVYEPSFPSPAFKPVSLKSYAVEKSPEGEGLQLLKPPPVPKRLGLPKSDGDQPFLPTPKSPSDRELKSSQEERRDLSSSSGLGLHGSSSNMKTLGSQSFNTSDSTMLTPPSSPPPPPPQDEEPATLRRKPYQTHEHKDAEPKASVIPPPPPATFMRAPREPTQPLQEEMRKSFVESVDEIPFADDVEDTYDDKTEDSSLQEKFFTPPSYWPRTERPLHPPLAKENGRLPTLESGVQPQKRGLPLVSPEAKELAEERMRAREKSVKSQALRDAMAKQLSKMKEMEMAAGTSSPPGGTSHKASSVPSKGKDLSPESPRRPVLKGPREPALKHEATSEEVLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRNKKEKKSKGEGRPPEKPSPSLLEEAAAKPKSLWKSVFSGYKKDKKKKGDDKSCSSTPSSGATVDSGKHKMSPVVRAELHLRRQLSFSEDSDLSSDDILERSSQKSRRESVYVPHALAFKRSYPSKPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELSEEKKILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAVMLSKGFSLNWS

Target ORF information:

RefSeq Version	XM_022410875.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022410875.1

ORF Insert Sequence:

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTGCCTGAC CTTCCCTCCA TACGCCGCGC AGCAGTGCAG 
GCCTGGCTGG AGACGGTCTC TGGAGTCCCA TTTGATGAGA ATGACCTGGA GGAAGACGTG 
GACTCGGAGC CAGCAAAGAT AGAAGGGGAG GCCACAGAGA ATGGGGACAC AGGGGACACT 
GGTGCTGAGC TGGACGATGA TCAGCGCTGG TCTGACGGGA TCCCATCGGA TGCCGAGACG 
GATCTCCGCG TGCAGCACCA GGAGGCGGTA GAGGCAGACC TGGAGCTGAG GGTCTCAGAG 
AATGAGGAGG AGCCACCTGA GGTCACACCA GGGCATCCGG AGAGAAGTCT CACCCGGGTC 
CCCAGCCCTG CCCTGTCCCC TGAGGAGCAA GCTGGTCTGC GGTCCCCAGG CCACAGCCCT 
AAGGCAGAGG GGGCCCAAAT CCCACTTGTC TCTGTTGTCA CCAAGGCAAA ATTGCCTGAA 
GAGAACCTTT TCCCCAAGCC TTTGCTCCCC AAAGTGAAGC CCAAGGTGGA AGTGCCCTGC 
AGTCAGAGAG CTGCATGCTC CCCCATCCGT TCTCAGCCAG TGGCTCTGCC AGAAGCCAGG 
ACACCTACCT CCCCAGTGAG CTCGCAGTGC CTGTCGCCTT TGGCCACCTC CACCCCCACC 
AGCACCCAGC TCCCCATTTG CTCCCAGCCT CAGCCTTCCT CTGAGACCAC GGCCCCATCC 
CCCACCAAGT CCCCCATACG GCTCCAGCCT GTTTCAGCCA AAACATCTAT CCCAGTGGTT 
CCCCTCCCCT TGAAGAACCA AGGGGACACC AAGGACAGGC TGGGCAGCCC TCTCGCTGTG 
GATGAGGCCC TGAAGCGGAA TGACCTGGTG GCAGAGTTCT GGATGAAGAG CGCCGAGATC 
CGCCGCAGCC TCGGGCTCAC ACCAGTGGAT CGGAGCAAGG TGTATGAGCC CAGCTTCCCT 
TCGCCTGCCT TTAAACCAGT GTCCCTAAAA TCCTATGCAG TGGAGAAGTC TCCCGAGGGG 
GAGGGACTCC AGCTTCTGAA GCCTCCACCT GTTCCTAAGA GACTAGGCCT GCCGAAGTCA 
GACGGTGACC AGCCCTTCCT GCCAACCCCC AAGTCCCCGT CGGACAGAGA GCTGAAAAGC 
TCCCAGGAGG AGAGGAGAGA TCTGTCGAGC AGCTCCGGGC TGGGCCTTCA CGGGAGCTCC 
TCCAACATGA AGACTTTGGG CAGCCAGAGC TTCAACACAT CAGACTCCAC CATGCTCACT 
CCCCCTTCAA GCCCACCGCC ACCACCACCC CAAGACGAGG AACCCGCAAC TCTTCGGAGG 
AAGCCATATC AGACTCATGA GCATAAGGAC GCTGAGCCCA AGGCCTCGGT GATTCCACCA 
CCACCACCTG CGACGTTCAT GCGGGCCCCC CGGGAGCCCA CCCAGCCCCT CCAAGAGGAG 
ATGCGGAAGT CGTTTGTGGA GAGCGTGGAT GAGATCCCAT TTGCCGATGA TGTGGAGGAC 
ACCTATGATG ACAAGACCGA GGACTCGAGT CTCCAGGAGA AGTTCTTCAC ACCCCCCTCC 
TACTGGCCCC GCACGGAGAG GCCCCTCCAC CCACCCCTAG CCAAGGAGAA TGGTAGGTTG 
CCTACTCTGG AGAGTGGGGT CCAGCCACAG AAGAGGGGCC TGCCCTTAGT CTCTCCAGAA 
GCCAAGGAGC TGGCTGAGGA GCGCATGCGA GCCAGGGAGA AGTCTGTGAA GAGCCAGGCG 
TTGAGAGACG CTATGGCCAA GCAGCTGAGT AAGATGAAGG AAATGGAGAT GGCGGCTGGG 
ACCTCCAGTC CCCCAGGAGG CACCTCCCAC AAAGCCTCCT CTGTTCCCTC CAAGGGCAAA 
GACCTCAGCC CCGAGTCCCC TAGACGCCCA GTTCTCAAAG GCCCCAGGGA GCCTGCCCTG 
AAGCACGAAG CCACTAGTGA GGAGGTCCTC TCCCCACCAT CGGACTCAGG GGGCCCAGAT 
GGTTCGGTCA CCTCATCCGA GGGCTCCAGT GGGAAGAGCA AGAAGAGATC GTCACTCTTC 
TCCCCCCGCA GAAACAAGAA AGAGAAGAAG TCCAAAGGTG AGGGCCGGCC CCCGGAGAAG 
CCCAGCCCAA GCCTTCTGGA GGAAGCTGCT GCCAAGCCCA AGTCCCTGTG GAAGTCGGTC 
TTTTCCGGGT ACAAGAAGGA CAAAAAGAAG AAGGGTGATG ACAAGTCCTG TTCCAGCACC 
CCTTCCAGCG GTGCCACTGT GGACTCTGGC AAGCACAAGA TGTCCCCTGT TGTGAGAGCA 
GAGCTGCACC TGCGGCGCCA GTTGAGCTTC TCCGAGGACT CAGACCTGTC CAGTGATGAC 
ATCCTTGAGA GGTCCTCCCA GAAGTCCAGG CGAGAGTCGG TTTATGTACC ACACGCCTTG 
GCATTCAAGA GATCATATCC ATCAAAGCCG AGAACCTACA CAGAGGAGGA ACTGAATGCC 
AAGCTGACCC GGCGTGTGCA GAAGGCAGCT CGTAGACAGG CCAAGCAAGA GGAGCTGAAG 
CGGCTGCACC GAGCACAGAT CATCCAGAGG CAGCTGGAGC AGGTGGAGGA GAAGCAGAGA 
CAGCTGGAGG AAAGAGGGGT GGCCGTGGAG AAGGCGCTCC GTGGTGAAGC AGACTATTGG 
GGAGAGTCTT ATTACAGTGA CCTCATCGAC TTGCATCTGG GTGTTGAGCC CAGTGGCGGG 
ACTCCACGGC GTAGACCTCT CTCCTTCTGC CCTTGCTGTG TCCAGGAAGG CATGGGCAAG 
AAGGATGACC CCAAGCTGAT GCAGGAGTGG TTCAAGTTGG TGCAAGAGAA GAATGCCATG 
GTGCGCTATG AGTCGGAGCT GATGATTTTT GCCCGGGAGC TGGAACTGGA GGACCGGCAG 
AGCCGACTGC AGCAGGAGCT CCGGGAGCGG ATGGCGGTGG AAGATCACCT GAAGACTGAG 
GAGGAGCTGT CTGAGGAGAA GAAGATCTTG AACGAGATGC TGGAGGTGGT GGAGCAGAGA 
GATGCCCTCG TGGCCCTGCT TGAGGAGCAG CGACTCCGGG AGAAGGAGGA GGACAAAGAC 
CTGGAGGCTG TCATGCTGTC CAAGGGCTTC AGCCTGAACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_014108385.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963860.1 Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X3, mRNA.

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CloneID	OCe43613
Clone ID Related Accession (Same CDS sequence)	XM_014108387.2 , XM_014108388.2 , XM_014108383.1 , XM_014108384.1 , XM_014108383.2 , XM_022410874.1 , XM_014108386.1 , XM_014108386.2 , XM_022410875.1 , XM_014108385.1 , XM_014108387.1 , XM_014108388.1
Accession Version	XM_014108385.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTGCCTGAC CTTCCCTCCA TACGCCGCGC AGCAGTGCAG 
GCCTGGCTGG AGACGGTCTC TGGAGTCCCA TTTGATGAGA ATGACCTGGA GGAAGACGTG 
GACTCGGAGC CAGCAAAGAT AGAAGGGGAG GCCACAGAGA ATGGGGACAC AGGGGACACT 
GGTGCTGAGC TGGACGATGA TCAGCGCTGG TCTGACGGGA TCCCATCGGA TGCCGAGACG 
GATCTCCGCG TGCAGCACCA GGAGGCGGTA GAGGCAGACC TGGAGCTGAG GGTCTCAGAG 
AATGAGGAGG AGCCACCTGA GGTCACACCA GGGCATCCGG AGAGAAGTCT CACCCGGGTC 
CCCAGCCCTG CCCTGTCCCC TGAGGAGCAA GCTGGTCTGC GGTCCCCAGG CCACAGCCCT 
AAGGCAGAGG GGGCCCAAAT CCCACTTGTC TCTGTTGTCA CCAAGGCAAA ATTGCCTGAA 
GAGAACCTTT TCCCCAAGCC TTTGCTCCCC AAAGTGAAGC CCAAGGTGGA AGTGCCCTGC 
AGTCAGAGAG CTGCATGCTC CCCCATCCGT TCTCAGCCAG TGGCTCTGCC AGAAGCCAGG 
ACACCTACCT CCCCAGTGAG CTCGCAGTGC CTGTCGCCTT TGGCCACCTC CACCCCCACC 
AGCACCCAGC TCCCCATTTG CTCCCAGCCT CAGCCTTCCT CTGAGACCAC GGCCCCATCC 
CCCACCAAGT CCCCCATACG GCTCCAGCCT GTTTCAGCCA AAACATCTAT CCCAGTGGTT 
CCCCTCCCCT TGAAGAACCA AGGGGACACC AAGGACAGGC TGGGCAGCCC TCTCGCTGTG 
GATGAGGCCC TGAAGCGGAA TGACCTGGTG GCAGAGTTCT GGATGAAGAG CGCCGAGATC 
CGCCGCAGCC TCGGGCTCAC ACCAGTGGAT CGGAGCAAGG TGTATGAGCC CAGCTTCCCT 
TCGCCTGCCT TTAAACCAGT GTCCCTAAAA TCCTATGCAG TGGAGAAGTC TCCCGAGGGG 
GAGGGACTCC AGCTTCTGAA GCCTCCACCT GTTCCTAAGA GACTAGGCCT GCCGAAGTCA 
GACGGTGACC AGCCCTTCCT GCCAACCCCC AAGTCCCCGT CGGACAGAGA GCTGAAAAGC 
TCCCAGGAGG AGAGGAGAGA TCTGTCGAGC AGCTCCGGGC TGGGCCTTCA CGGGAGCTCC 
TCCAACATGA AGACTTTGGG CAGCCAGAGC TTCAACACAT CAGACTCCAC CATGCTCACT 
CCCCCTTCAA GCCCACCGCC ACCACCACCC CAAGACGAGG AACCCGCAAC TCTTCGGAGG 
AAGCCATATC AGACTCATGA GCATAAGGAC GCTGAGCCCA AGGCCTCGGT GATTCCACCA 
CCACCACCTG CGACGTTCAT GCGGGCCCCC CGGGAGCCCA CCCAGCCCCT CCAAGAGGAG 
ATGCGGAAGT CGTTTGTGGA GAGCGTGGAT GAGATCCCAT TTGCCGATGA TGTGGAGGAC 
ACCTATGATG ACAAGACCGA GGACTCGAGT CTCCAGGAGA AGTTCTTCAC ACCCCCCTCC 
TACTGGCCCC GCACGGAGAG GCCCCTCCAC CCACCCCTAG CCAAGGAGAA TGGTAGGTTG 
CCTACTCTGG AGAGTGGGGT CCAGCCACAG AAGAGGGGCC TGCCCTTAGT CTCTCCAGAA 
GCCAAGGAGC TGGCTGAGGA GCGCATGCGA GCCAGGGAGA AGTCTGTGAA GAGCCAGGCG 
TTGAGAGACG CTATGGCCAA GCAGCTGAGT AAGATGAAGG AAATGGAGAT GGCGGCTGGG 
ACCTCCAGTC CCCCAGGAGG CACCTCCCAC AAAGCCTCCT CTGTTCCCTC CAAGGGCAAA 
GACCTCAGCC CCGAGTCCCC TAGACGCCCA GTTCTCAAAG GCCCCAGGGA GCCTGCCCTG 
AAGCACGAAG CCACTAGTGA GGAGGTCCTC TCCCCACCAT CGGACTCAGG GGGCCCAGAT 
GGTTCGGTCA CCTCATCCGA GGGCTCCAGT GGGAAGAGCA AGAAGAGATC GTCACTCTTC 
TCCCCCCGCA GAAACAAGAA AGAGAAGAAG TCCAAAGGTG AGGGCCGGCC CCCGGAGAAG 
CCCAGCCCAA GCCTTCTGGA GGAAGCTGCT GCCAAGCCCA AGTCCCTGTG GAAGTCGGTC 
TTTTCCGGGT ACAAGAAGGA CAAAAAGAAG AAGGGTGATG ACAAGTCCTG TTCCAGCACC 
CCTTCCAGCG GTGCCACTGT GGACTCTGGC AAGCACAAGA TGTCCCCTGT TGTGAGAGCA 
GAGCTGCACC TGCGGCGCCA GTTGAGCTTC TCCGAGGACT CAGACCTGTC CAGTGATGAC 
ATCCTTGAGA GGTCCTCCCA GAAGTCCAGG CGAGAGTCGG TTTATGTACC ACACGCCTTG 
GCATTCAAGA GATCATATCC ATCAAAGCCG AGAACCTACA CAGAGGAGGA ACTGAATGCC 
AAGCTGACCC GGCGTGTGCA GAAGGCAGCT CGTAGACAGG CCAAGCAAGA GGAGCTGAAG 
CGGCTGCACC GAGCACAGAT CATCCAGAGG CAGCTGGAGC AGGTGGAGGA GAAGCAGAGA 
CAGCTGGAGG AAAGAGGGGT GGCCGTGGAG AAGGCGCTCC GTGGTGAAGC AGACTATTGG 
GGAGAGTCTT ATTACAGTGA CCTCATCGAC TTGCATCTGG GTGTTGAGCC CAGTGGCGGG 
ACTCCACGGC GTAGACCTCT CTCCTTCTGC CCTTGCTGTG TCCAGGAAGG CATGGGCAAG 
AAGGATGACC CCAAGCTGAT GCAGGAGTGG TTCAAGTTGG TGCAAGAGAA GAATGCCATG 
GTGCGCTATG AGTCGGAGCT GATGATTTTT GCCCGGGAGC TGGAACTGGA GGACCGGCAG 
AGCCGACTGC AGCAGGAGCT CCGGGAGCGG ATGGCGGTGG AAGATCACCT GAAGACTGAG 
GAGGAGCTGT CTGAGGAGAA GAAGATCTTG AACGAGATGC TGGAGGTGGT GGAGCAGAGA 
GATGCCCTCG TGGCCCTGCT TGAGGAGCAG CGACTCCGGG AGAAGGAGGA GGACAAAGAC 
CTGGAGGCTG TCATGCTGTC CAAGGGCTTC AGCCTGAACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963860.1
CDS	1214..8041
Translation	MEDSKNETVNHAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDYRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKACTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFQGLVQPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELKEATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLNYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREQNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNININFLRPSQVRHLYNTGETRDIHLEMENLVNSRTTPKLTRNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGKEMASVGEPDKLSMVMYLTQFYEMFKDSLPSSDALDLNAEEKAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEEAPRGYRGGRPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSTGMRRQPTQERGVSQPSCCLPEQGHPAPTPQWKQQQEKERSRTCPKKVITLSPPLTPPPCRAPRSQQTYRDLDADSRGKQSPHHERPEPEPSRRFFVDQWELSLSLRSSNRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCRKRVYVMERLSAEGKFFHRSCFKCEYCATTLRLSAYAYDIEDGKFYCKPHYCYRLSGSAQRKRPAVAPLSGKEAREPLQDGPTVDTNGRASAVTSPAERTPGSSVNGVEEPSIAKRLRGTPERIELENYRLSVKQAEELEEVPEETQAEHNLSSVLDTGAEEDVASSSSESEMEEEEEEEPPLPTSDLGGVPWKEAVRIHALLKGKSEEELEASKSFGPGHEEDEEEEEYEEEEEEEEEEYEEEEEEEESSEEGEYCPWDRELLQGQWLRQLSQEETTGTRKVGSQRLQQIVNPADPLEIQADVHWTHIREREEEERMVPTSESSTSRVPDLPSIRRAAVQAWLETVSGVPFDENDLEEDVDSEPAKIEGEATENGDTGDTGAELDDDQRWSDGIPSDAETDLRVQHQEAVEADLELRVSENEEEPPEVTPGHPERSLTRVPSPALSPEEQAGLRSPGHSPKAEGAQIPLVSVVTKAKLPEENLFPKPLLPKVKPKVEVPCSQRAACSPIRSQPVALPEARTPTSPVSSQCLSPLATSTPTSTQLPICSQPQPSSETTAPSPTKSPIRLQPVSAKTSIPVVPLPLKNQGDTKDRLGSPLAVDEALKRNDLVAEFWMKSAEIRRSLGLTPVDRSKVYEPSFPSPAFKPVSLKSYAVEKSPEGEGLQLLKPPPVPKRLGLPKSDGDQPFLPTPKSPSDRELKSSQEERRDLSSSSGLGLHGSSSNMKTLGSQSFNTSDSTMLTPPSSPPPPPPQDEEPATLRRKPYQTHEHKDAEPKASVIPPPPPATFMRAPREPTQPLQEEMRKSFVESVDEIPFADDVEDTYDDKTEDSSLQEKFFTPPSYWPRTERPLHPPLAKENGRLPTLESGVQPQKRGLPLVSPEAKELAEERMRAREKSVKSQALRDAMAKQLSKMKEMEMAAGTSSPPGGTSHKASSVPSKGKDLSPESPRRPVLKGPREPALKHEATSEEVLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRNKKEKKSKGEGRPPEKPSPSLLEEAAAKPKSLWKSVFSGYKKDKKKKGDDKSCSSTPSSGATVDSGKHKMSPVVRAELHLRRQLSFSEDSDLSSDDILERSSQKSRRESVYVPHALAFKRSYPSKPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELSEEKKILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAVMLSKGFSLNWS

Target ORF information:

RefSeq Version	XM_014108385.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108385.1

ORF Insert Sequence:

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTGCCTGAC CTTCCCTCCA TACGCCGCGC AGCAGTGCAG 
GCCTGGCTGG AGACGGTCTC TGGAGTCCCA TTTGATGAGA ATGACCTGGA GGAAGACGTG 
GACTCGGAGC CAGCAAAGAT AGAAGGGGAG GCCACAGAGA ATGGGGACAC AGGGGACACT 
GGTGCTGAGC TGGACGATGA TCAGCGCTGG TCTGACGGGA TCCCATCGGA TGCCGAGACG 
GATCTCCGCG TGCAGCACCA GGAGGCGGTA GAGGCAGACC TGGAGCTGAG GGTCTCAGAG 
AATGAGGAGG AGCCACCTGA GGTCACACCA GGGCATCCGG AGAGAAGTCT CACCCGGGTC 
CCCAGCCCTG CCCTGTCCCC TGAGGAGCAA GCTGGTCTGC GGTCCCCAGG CCACAGCCCT 
AAGGCAGAGG GGGCCCAAAT CCCACTTGTC TCTGTTGTCA CCAAGGCAAA ATTGCCTGAA 
GAGAACCTTT TCCCCAAGCC TTTGCTCCCC AAAGTGAAGC CCAAGGTGGA AGTGCCCTGC 
AGTCAGAGAG CTGCATGCTC CCCCATCCGT TCTCAGCCAG TGGCTCTGCC AGAAGCCAGG 
ACACCTACCT CCCCAGTGAG CTCGCAGTGC CTGTCGCCTT TGGCCACCTC CACCCCCACC 
AGCACCCAGC TCCCCATTTG CTCCCAGCCT CAGCCTTCCT CTGAGACCAC GGCCCCATCC 
CCCACCAAGT CCCCCATACG GCTCCAGCCT GTTTCAGCCA AAACATCTAT CCCAGTGGTT 
CCCCTCCCCT TGAAGAACCA AGGGGACACC AAGGACAGGC TGGGCAGCCC TCTCGCTGTG 
GATGAGGCCC TGAAGCGGAA TGACCTGGTG GCAGAGTTCT GGATGAAGAG CGCCGAGATC 
CGCCGCAGCC TCGGGCTCAC ACCAGTGGAT CGGAGCAAGG TGTATGAGCC CAGCTTCCCT 
TCGCCTGCCT TTAAACCAGT GTCCCTAAAA TCCTATGCAG TGGAGAAGTC TCCCGAGGGG 
GAGGGACTCC AGCTTCTGAA GCCTCCACCT GTTCCTAAGA GACTAGGCCT GCCGAAGTCA 
GACGGTGACC AGCCCTTCCT GCCAACCCCC AAGTCCCCGT CGGACAGAGA GCTGAAAAGC 
TCCCAGGAGG AGAGGAGAGA TCTGTCGAGC AGCTCCGGGC TGGGCCTTCA CGGGAGCTCC 
TCCAACATGA AGACTTTGGG CAGCCAGAGC TTCAACACAT CAGACTCCAC CATGCTCACT 
CCCCCTTCAA GCCCACCGCC ACCACCACCC CAAGACGAGG AACCCGCAAC TCTTCGGAGG 
AAGCCATATC AGACTCATGA GCATAAGGAC GCTGAGCCCA AGGCCTCGGT GATTCCACCA 
CCACCACCTG CGACGTTCAT GCGGGCCCCC CGGGAGCCCA CCCAGCCCCT CCAAGAGGAG 
ATGCGGAAGT CGTTTGTGGA GAGCGTGGAT GAGATCCCAT TTGCCGATGA TGTGGAGGAC 
ACCTATGATG ACAAGACCGA GGACTCGAGT CTCCAGGAGA AGTTCTTCAC ACCCCCCTCC 
TACTGGCCCC GCACGGAGAG GCCCCTCCAC CCACCCCTAG CCAAGGAGAA TGGTAGGTTG 
CCTACTCTGG AGAGTGGGGT CCAGCCACAG AAGAGGGGCC TGCCCTTAGT CTCTCCAGAA 
GCCAAGGAGC TGGCTGAGGA GCGCATGCGA GCCAGGGAGA AGTCTGTGAA GAGCCAGGCG 
TTGAGAGACG CTATGGCCAA GCAGCTGAGT AAGATGAAGG AAATGGAGAT GGCGGCTGGG 
ACCTCCAGTC CCCCAGGAGG CACCTCCCAC AAAGCCTCCT CTGTTCCCTC CAAGGGCAAA 
GACCTCAGCC CCGAGTCCCC TAGACGCCCA GTTCTCAAAG GCCCCAGGGA GCCTGCCCTG 
AAGCACGAAG CCACTAGTGA GGAGGTCCTC TCCCCACCAT CGGACTCAGG GGGCCCAGAT 
GGTTCGGTCA CCTCATCCGA GGGCTCCAGT GGGAAGAGCA AGAAGAGATC GTCACTCTTC 
TCCCCCCGCA GAAACAAGAA AGAGAAGAAG TCCAAAGGTG AGGGCCGGCC CCCGGAGAAG 
CCCAGCCCAA GCCTTCTGGA GGAAGCTGCT GCCAAGCCCA AGTCCCTGTG GAAGTCGGTC 
TTTTCCGGGT ACAAGAAGGA CAAAAAGAAG AAGGGTGATG ACAAGTCCTG TTCCAGCACC 
CCTTCCAGCG GTGCCACTGT GGACTCTGGC AAGCACAAGA TGTCCCCTGT TGTGAGAGCA 
GAGCTGCACC TGCGGCGCCA GTTGAGCTTC TCCGAGGACT CAGACCTGTC CAGTGATGAC 
ATCCTTGAGA GGTCCTCCCA GAAGTCCAGG CGAGAGTCGG TTTATGTACC ACACGCCTTG 
GCATTCAAGA GATCATATCC ATCAAAGCCG AGAACCTACA CAGAGGAGGA ACTGAATGCC 
AAGCTGACCC GGCGTGTGCA GAAGGCAGCT CGTAGACAGG CCAAGCAAGA GGAGCTGAAG 
CGGCTGCACC GAGCACAGAT CATCCAGAGG CAGCTGGAGC AGGTGGAGGA GAAGCAGAGA 
CAGCTGGAGG AAAGAGGGGT GGCCGTGGAG AAGGCGCTCC GTGGTGAAGC AGACTATTGG 
GGAGAGTCTT ATTACAGTGA CCTCATCGAC TTGCATCTGG GTGTTGAGCC CAGTGGCGGG 
ACTCCACGGC GTAGACCTCT CTCCTTCTGC CCTTGCTGTG TCCAGGAAGG CATGGGCAAG 
AAGGATGACC CCAAGCTGAT GCAGGAGTGG TTCAAGTTGG TGCAAGAGAA GAATGCCATG 
GTGCGCTATG AGTCGGAGCT GATGATTTTT GCCCGGGAGC TGGAACTGGA GGACCGGCAG 
AGCCGACTGC AGCAGGAGCT CCGGGAGCGG ATGGCGGTGG AAGATCACCT GAAGACTGAG 
GAGGAGCTGT CTGAGGAGAA GAAGATCTTG AACGAGATGC TGGAGGTGGT GGAGCAGAGA 
GATGCCCTCG TGGCCCTGCT TGAGGAGCAG CGACTCCGGG AGAAGGAGGA GGACAAAGAC 
CTGGAGGCTG TCATGCTGTC CAAGGGCTTC AGCCTGAACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_014108387.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963862.1 Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X5, mRNA.

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CloneID	OCe43613
Clone ID Related Accession (Same CDS sequence)	XM_014108387.2 , XM_014108388.2 , XM_014108383.1 , XM_014108384.1 , XM_014108383.2 , XM_022410874.1 , XM_014108386.1 , XM_014108386.2 , XM_022410875.1 , XM_014108385.1 , XM_014108387.1 , XM_014108388.1
Accession Version	XM_014108387.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTGCCTGAC CTTCCCTCCA TACGCCGCGC AGCAGTGCAG 
GCCTGGCTGG AGACGGTCTC TGGAGTCCCA TTTGATGAGA ATGACCTGGA GGAAGACGTG 
GACTCGGAGC CAGCAAAGAT AGAAGGGGAG GCCACAGAGA ATGGGGACAC AGGGGACACT 
GGTGCTGAGC TGGACGATGA TCAGCGCTGG TCTGACGGGA TCCCATCGGA TGCCGAGACG 
GATCTCCGCG TGCAGCACCA GGAGGCGGTA GAGGCAGACC TGGAGCTGAG GGTCTCAGAG 
AATGAGGAGG AGCCACCTGA GGTCACACCA GGGCATCCGG AGAGAAGTCT CACCCGGGTC 
CCCAGCCCTG CCCTGTCCCC TGAGGAGCAA GCTGGTCTGC GGTCCCCAGG CCACAGCCCT 
AAGGCAGAGG GGGCCCAAAT CCCACTTGTC TCTGTTGTCA CCAAGGCAAA ATTGCCTGAA 
GAGAACCTTT TCCCCAAGCC TTTGCTCCCC AAAGTGAAGC CCAAGGTGGA AGTGCCCTGC 
AGTCAGAGAG CTGCATGCTC CCCCATCCGT TCTCAGCCAG TGGCTCTGCC AGAAGCCAGG 
ACACCTACCT CCCCAGTGAG CTCGCAGTGC CTGTCGCCTT TGGCCACCTC CACCCCCACC 
AGCACCCAGC TCCCCATTTG CTCCCAGCCT CAGCCTTCCT CTGAGACCAC GGCCCCATCC 
CCCACCAAGT CCCCCATACG GCTCCAGCCT GTTTCAGCCA AAACATCTAT CCCAGTGGTT 
CCCCTCCCCT TGAAGAACCA AGGGGACACC AAGGACAGGC TGGGCAGCCC TCTCGCTGTG 
GATGAGGCCC TGAAGCGGAA TGACCTGGTG GCAGAGTTCT GGATGAAGAG CGCCGAGATC 
CGCCGCAGCC TCGGGCTCAC ACCAGTGGAT CGGAGCAAGG TGTATGAGCC CAGCTTCCCT 
TCGCCTGCCT TTAAACCAGT GTCCCTAAAA TCCTATGCAG TGGAGAAGTC TCCCGAGGGG 
GAGGGACTCC AGCTTCTGAA GCCTCCACCT GTTCCTAAGA GACTAGGCCT GCCGAAGTCA 
GACGGTGACC AGCCCTTCCT GCCAACCCCC AAGTCCCCGT CGGACAGAGA GCTGAAAAGC 
TCCCAGGAGG AGAGGAGAGA TCTGTCGAGC AGCTCCGGGC TGGGCCTTCA CGGGAGCTCC 
TCCAACATGA AGACTTTGGG CAGCCAGAGC TTCAACACAT CAGACTCCAC CATGCTCACT 
CCCCCTTCAA GCCCACCGCC ACCACCACCC CAAGACGAGG AACCCGCAAC TCTTCGGAGG 
AAGCCATATC AGACTCATGA GCATAAGGAC GCTGAGCCCA AGGCCTCGGT GATTCCACCA 
CCACCACCTG CGACGTTCAT GCGGGCCCCC CGGGAGCCCA CCCAGCCCCT CCAAGAGGAG 
ATGCGGAAGT CGTTTGTGGA GAGCGTGGAT GAGATCCCAT TTGCCGATGA TGTGGAGGAC 
ACCTATGATG ACAAGACCGA GGACTCGAGT CTCCAGGAGA AGTTCTTCAC ACCCCCCTCC 
TACTGGCCCC GCACGGAGAG GCCCCTCCAC CCACCCCTAG CCAAGGAGAA TGGTAGGTTG 
CCTACTCTGG AGAGTGGGGT CCAGCCACAG AAGAGGGGCC TGCCCTTAGT CTCTCCAGAA 
GCCAAGGAGC TGGCTGAGGA GCGCATGCGA GCCAGGGAGA AGTCTGTGAA GAGCCAGGCG 
TTGAGAGACG CTATGGCCAA GCAGCTGAGT AAGATGAAGG AAATGGAGAT GGCGGCTGGG 
ACCTCCAGTC CCCCAGGAGG CACCTCCCAC AAAGCCTCCT CTGTTCCCTC CAAGGGCAAA 
GACCTCAGCC CCGAGTCCCC TAGACGCCCA GTTCTCAAAG GCCCCAGGGA GCCTGCCCTG 
AAGCACGAAG CCACTAGTGA GGAGGTCCTC TCCCCACCAT CGGACTCAGG GGGCCCAGAT 
GGTTCGGTCA CCTCATCCGA GGGCTCCAGT GGGAAGAGCA AGAAGAGATC GTCACTCTTC 
TCCCCCCGCA GAAACAAGAA AGAGAAGAAG TCCAAAGGTG AGGGCCGGCC CCCGGAGAAG 
CCCAGCCCAA GCCTTCTGGA GGAAGCTGCT GCCAAGCCCA AGTCCCTGTG GAAGTCGGTC 
TTTTCCGGGT ACAAGAAGGA CAAAAAGAAG AAGGGTGATG ACAAGTCCTG TTCCAGCACC 
CCTTCCAGCG GTGCCACTGT GGACTCTGGC AAGCACAAGA TGTCCCCTGT TGTGAGAGCA 
GAGCTGCACC TGCGGCGCCA GTTGAGCTTC TCCGAGGACT CAGACCTGTC CAGTGATGAC 
ATCCTTGAGA GGTCCTCCCA GAAGTCCAGG CGAGAGTCGG TTTATGTACC ACACGCCTTG 
GCATTCAAGA GATCATATCC ATCAAAGCCG AGAACCTACA CAGAGGAGGA ACTGAATGCC 
AAGCTGACCC GGCGTGTGCA GAAGGCAGCT CGTAGACAGG CCAAGCAAGA GGAGCTGAAG 
CGGCTGCACC GAGCACAGAT CATCCAGAGG CAGCTGGAGC AGGTGGAGGA GAAGCAGAGA 
CAGCTGGAGG AAAGAGGGGT GGCCGTGGAG AAGGCGCTCC GTGGTGAAGC AGACTATTGG 
GGAGAGTCTT ATTACAGTGA CCTCATCGAC TTGCATCTGG GTGTTGAGCC CAGTGGCGGG 
ACTCCACGGC GTAGACCTCT CTCCTTCTGC CCTTGCTGTG TCCAGGAAGG CATGGGCAAG 
AAGGATGACC CCAAGCTGAT GCAGGAGTGG TTCAAGTTGG TGCAAGAGAA GAATGCCATG 
GTGCGCTATG AGTCGGAGCT GATGATTTTT GCCCGGGAGC TGGAACTGGA GGACCGGCAG 
AGCCGACTGC AGCAGGAGCT CCGGGAGCGG ATGGCGGTGG AAGATCACCT GAAGACTGAG 
GAGGAGCTGT CTGAGGAGAA GAAGATCTTG AACGAGATGC TGGAGGTGGT GGAGCAGAGA 
GATGCCCTCG TGGCCCTGCT TGAGGAGCAG CGACTCCGGG AGAAGGAGGA GGACAAAGAC 
CTGGAGGCTG TCATGCTGTC CAAGGGCTTC AGCCTGAACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963862.1
CDS	282..7109
Translation	MEDSKNETVNHAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDYRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKACTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFQGLVQPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELKEATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLNYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREQNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNININFLRPSQVRHLYNTGETRDIHLEMENLVNSRTTPKLTRNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGKEMASVGEPDKLSMVMYLTQFYEMFKDSLPSSDALDLNAEEKAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEEAPRGYRGGRPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSTGMRRQPTQERGVSQPSCCLPEQGHPAPTPQWKQQQEKERSRTCPKKVITLSPPLTPPPCRAPRSQQTYRDLDADSRGKQSPHHERPEPEPSRRFFVDQWELSLSLRSSNRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCRKRVYVMERLSAEGKFFHRSCFKCEYCATTLRLSAYAYDIEDGKFYCKPHYCYRLSGSAQRKRPAVAPLSGKEAREPLQDGPTVDTNGRASAVTSPAERTPGSSVNGVEEPSIAKRLRGTPERIELENYRLSVKQAEELEEVPEETQAEHNLSSVLDTGAEEDVASSSSESEMEEEEEEEPPLPTSDLGGVPWKEAVRIHALLKGKSEEELEASKSFGPGHEEDEEEEEYEEEEEEEEEEYEEEEEEEESSEEGEYCPWDRELLQGQWLRQLSQEETTGTRKVGSQRLQQIVNPADPLEIQADVHWTHIREREEEERMVPTSESSTSRVPDLPSIRRAAVQAWLETVSGVPFDENDLEEDVDSEPAKIEGEATENGDTGDTGAELDDDQRWSDGIPSDAETDLRVQHQEAVEADLELRVSENEEEPPEVTPGHPERSLTRVPSPALSPEEQAGLRSPGHSPKAEGAQIPLVSVVTKAKLPEENLFPKPLLPKVKPKVEVPCSQRAACSPIRSQPVALPEARTPTSPVSSQCLSPLATSTPTSTQLPICSQPQPSSETTAPSPTKSPIRLQPVSAKTSIPVVPLPLKNQGDTKDRLGSPLAVDEALKRNDLVAEFWMKSAEIRRSLGLTPVDRSKVYEPSFPSPAFKPVSLKSYAVEKSPEGEGLQLLKPPPVPKRLGLPKSDGDQPFLPTPKSPSDRELKSSQEERRDLSSSSGLGLHGSSSNMKTLGSQSFNTSDSTMLTPPSSPPPPPPQDEEPATLRRKPYQTHEHKDAEPKASVIPPPPPATFMRAPREPTQPLQEEMRKSFVESVDEIPFADDVEDTYDDKTEDSSLQEKFFTPPSYWPRTERPLHPPLAKENGRLPTLESGVQPQKRGLPLVSPEAKELAEERMRAREKSVKSQALRDAMAKQLSKMKEMEMAAGTSSPPGGTSHKASSVPSKGKDLSPESPRRPVLKGPREPALKHEATSEEVLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRNKKEKKSKGEGRPPEKPSPSLLEEAAAKPKSLWKSVFSGYKKDKKKKGDDKSCSSTPSSGATVDSGKHKMSPVVRAELHLRRQLSFSEDSDLSSDDILERSSQKSRRESVYVPHALAFKRSYPSKPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELSEEKKILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAVMLSKGFSLNWS

Target ORF information:

RefSeq Version	XM_014108387.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108387.1

ORF Insert Sequence:

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTGCCTGAC CTTCCCTCCA TACGCCGCGC AGCAGTGCAG 
GCCTGGCTGG AGACGGTCTC TGGAGTCCCA TTTGATGAGA ATGACCTGGA GGAAGACGTG 
GACTCGGAGC CAGCAAAGAT AGAAGGGGAG GCCACAGAGA ATGGGGACAC AGGGGACACT 
GGTGCTGAGC TGGACGATGA TCAGCGCTGG TCTGACGGGA TCCCATCGGA TGCCGAGACG 
GATCTCCGCG TGCAGCACCA GGAGGCGGTA GAGGCAGACC TGGAGCTGAG GGTCTCAGAG 
AATGAGGAGG AGCCACCTGA GGTCACACCA GGGCATCCGG AGAGAAGTCT CACCCGGGTC 
CCCAGCCCTG CCCTGTCCCC TGAGGAGCAA GCTGGTCTGC GGTCCCCAGG CCACAGCCCT 
AAGGCAGAGG GGGCCCAAAT CCCACTTGTC TCTGTTGTCA CCAAGGCAAA ATTGCCTGAA 
GAGAACCTTT TCCCCAAGCC TTTGCTCCCC AAAGTGAAGC CCAAGGTGGA AGTGCCCTGC 
AGTCAGAGAG CTGCATGCTC CCCCATCCGT TCTCAGCCAG TGGCTCTGCC AGAAGCCAGG 
ACACCTACCT CCCCAGTGAG CTCGCAGTGC CTGTCGCCTT TGGCCACCTC CACCCCCACC 
AGCACCCAGC TCCCCATTTG CTCCCAGCCT CAGCCTTCCT CTGAGACCAC GGCCCCATCC 
CCCACCAAGT CCCCCATACG GCTCCAGCCT GTTTCAGCCA AAACATCTAT CCCAGTGGTT 
CCCCTCCCCT TGAAGAACCA AGGGGACACC AAGGACAGGC TGGGCAGCCC TCTCGCTGTG 
GATGAGGCCC TGAAGCGGAA TGACCTGGTG GCAGAGTTCT GGATGAAGAG CGCCGAGATC 
CGCCGCAGCC TCGGGCTCAC ACCAGTGGAT CGGAGCAAGG TGTATGAGCC CAGCTTCCCT 
TCGCCTGCCT TTAAACCAGT GTCCCTAAAA TCCTATGCAG TGGAGAAGTC TCCCGAGGGG 
GAGGGACTCC AGCTTCTGAA GCCTCCACCT GTTCCTAAGA GACTAGGCCT GCCGAAGTCA 
GACGGTGACC AGCCCTTCCT GCCAACCCCC AAGTCCCCGT CGGACAGAGA GCTGAAAAGC 
TCCCAGGAGG AGAGGAGAGA TCTGTCGAGC AGCTCCGGGC TGGGCCTTCA CGGGAGCTCC 
TCCAACATGA AGACTTTGGG CAGCCAGAGC TTCAACACAT CAGACTCCAC CATGCTCACT 
CCCCCTTCAA GCCCACCGCC ACCACCACCC CAAGACGAGG AACCCGCAAC TCTTCGGAGG 
AAGCCATATC AGACTCATGA GCATAAGGAC GCTGAGCCCA AGGCCTCGGT GATTCCACCA 
CCACCACCTG CGACGTTCAT GCGGGCCCCC CGGGAGCCCA CCCAGCCCCT CCAAGAGGAG 
ATGCGGAAGT CGTTTGTGGA GAGCGTGGAT GAGATCCCAT TTGCCGATGA TGTGGAGGAC 
ACCTATGATG ACAAGACCGA GGACTCGAGT CTCCAGGAGA AGTTCTTCAC ACCCCCCTCC 
TACTGGCCCC GCACGGAGAG GCCCCTCCAC CCACCCCTAG CCAAGGAGAA TGGTAGGTTG 
CCTACTCTGG AGAGTGGGGT CCAGCCACAG AAGAGGGGCC TGCCCTTAGT CTCTCCAGAA 
GCCAAGGAGC TGGCTGAGGA GCGCATGCGA GCCAGGGAGA AGTCTGTGAA GAGCCAGGCG 
TTGAGAGACG CTATGGCCAA GCAGCTGAGT AAGATGAAGG AAATGGAGAT GGCGGCTGGG 
ACCTCCAGTC CCCCAGGAGG CACCTCCCAC AAAGCCTCCT CTGTTCCCTC CAAGGGCAAA 
GACCTCAGCC CCGAGTCCCC TAGACGCCCA GTTCTCAAAG GCCCCAGGGA GCCTGCCCTG 
AAGCACGAAG CCACTAGTGA GGAGGTCCTC TCCCCACCAT CGGACTCAGG GGGCCCAGAT 
GGTTCGGTCA CCTCATCCGA GGGCTCCAGT GGGAAGAGCA AGAAGAGATC GTCACTCTTC 
TCCCCCCGCA GAAACAAGAA AGAGAAGAAG TCCAAAGGTG AGGGCCGGCC CCCGGAGAAG 
CCCAGCCCAA GCCTTCTGGA GGAAGCTGCT GCCAAGCCCA AGTCCCTGTG GAAGTCGGTC 
TTTTCCGGGT ACAAGAAGGA CAAAAAGAAG AAGGGTGATG ACAAGTCCTG TTCCAGCACC 
CCTTCCAGCG GTGCCACTGT GGACTCTGGC AAGCACAAGA TGTCCCCTGT TGTGAGAGCA 
GAGCTGCACC TGCGGCGCCA GTTGAGCTTC TCCGAGGACT CAGACCTGTC CAGTGATGAC 
ATCCTTGAGA GGTCCTCCCA GAAGTCCAGG CGAGAGTCGG TTTATGTACC ACACGCCTTG 
GCATTCAAGA GATCATATCC ATCAAAGCCG AGAACCTACA CAGAGGAGGA ACTGAATGCC 
AAGCTGACCC GGCGTGTGCA GAAGGCAGCT CGTAGACAGG CCAAGCAAGA GGAGCTGAAG 
CGGCTGCACC GAGCACAGAT CATCCAGAGG CAGCTGGAGC AGGTGGAGGA GAAGCAGAGA 
CAGCTGGAGG AAAGAGGGGT GGCCGTGGAG AAGGCGCTCC GTGGTGAAGC AGACTATTGG 
GGAGAGTCTT ATTACAGTGA CCTCATCGAC TTGCATCTGG GTGTTGAGCC CAGTGGCGGG 
ACTCCACGGC GTAGACCTCT CTCCTTCTGC CCTTGCTGTG TCCAGGAAGG CATGGGCAAG 
AAGGATGACC CCAAGCTGAT GCAGGAGTGG TTCAAGTTGG TGCAAGAGAA GAATGCCATG 
GTGCGCTATG AGTCGGAGCT GATGATTTTT GCCCGGGAGC TGGAACTGGA GGACCGGCAG 
AGCCGACTGC AGCAGGAGCT CCGGGAGCGG ATGGCGGTGG AAGATCACCT GAAGACTGAG 
GAGGAGCTGT CTGAGGAGAA GAAGATCTTG AACGAGATGC TGGAGGTGGT GGAGCAGAGA 
GATGCCCTCG TGGCCCTGCT TGAGGAGCAG CGACTCCGGG AGAAGGAGGA GGACAAAGAC 
CTGGAGGCTG TCATGCTGTC CAAGGGCTTC AGCCTGAACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_014108388.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963863.1 Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X6, mRNA.

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CloneID	OCe43613
Clone ID Related Accession (Same CDS sequence)	XM_014108387.2 , XM_014108388.2 , XM_014108383.1 , XM_014108384.1 , XM_014108383.2 , XM_022410874.1 , XM_014108386.1 , XM_014108386.2 , XM_022410875.1 , XM_014108385.1 , XM_014108387.1 , XM_014108388.1
Accession Version	XM_014108388.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTGCCTGAC CTTCCCTCCA TACGCCGCGC AGCAGTGCAG 
GCCTGGCTGG AGACGGTCTC TGGAGTCCCA TTTGATGAGA ATGACCTGGA GGAAGACGTG 
GACTCGGAGC CAGCAAAGAT AGAAGGGGAG GCCACAGAGA ATGGGGACAC AGGGGACACT 
GGTGCTGAGC TGGACGATGA TCAGCGCTGG TCTGACGGGA TCCCATCGGA TGCCGAGACG 
GATCTCCGCG TGCAGCACCA GGAGGCGGTA GAGGCAGACC TGGAGCTGAG GGTCTCAGAG 
AATGAGGAGG AGCCACCTGA GGTCACACCA GGGCATCCGG AGAGAAGTCT CACCCGGGTC 
CCCAGCCCTG CCCTGTCCCC TGAGGAGCAA GCTGGTCTGC GGTCCCCAGG CCACAGCCCT 
AAGGCAGAGG GGGCCCAAAT CCCACTTGTC TCTGTTGTCA CCAAGGCAAA ATTGCCTGAA 
GAGAACCTTT TCCCCAAGCC TTTGCTCCCC AAAGTGAAGC CCAAGGTGGA AGTGCCCTGC 
AGTCAGAGAG CTGCATGCTC CCCCATCCGT TCTCAGCCAG TGGCTCTGCC AGAAGCCAGG 
ACACCTACCT CCCCAGTGAG CTCGCAGTGC CTGTCGCCTT TGGCCACCTC CACCCCCACC 
AGCACCCAGC TCCCCATTTG CTCCCAGCCT CAGCCTTCCT CTGAGACCAC GGCCCCATCC 
CCCACCAAGT CCCCCATACG GCTCCAGCCT GTTTCAGCCA AAACATCTAT CCCAGTGGTT 
CCCCTCCCCT TGAAGAACCA AGGGGACACC AAGGACAGGC TGGGCAGCCC TCTCGCTGTG 
GATGAGGCCC TGAAGCGGAA TGACCTGGTG GCAGAGTTCT GGATGAAGAG CGCCGAGATC 
CGCCGCAGCC TCGGGCTCAC ACCAGTGGAT CGGAGCAAGG TGTATGAGCC CAGCTTCCCT 
TCGCCTGCCT TTAAACCAGT GTCCCTAAAA TCCTATGCAG TGGAGAAGTC TCCCGAGGGG 
GAGGGACTCC AGCTTCTGAA GCCTCCACCT GTTCCTAAGA GACTAGGCCT GCCGAAGTCA 
GACGGTGACC AGCCCTTCCT GCCAACCCCC AAGTCCCCGT CGGACAGAGA GCTGAAAAGC 
TCCCAGGAGG AGAGGAGAGA TCTGTCGAGC AGCTCCGGGC TGGGCCTTCA CGGGAGCTCC 
TCCAACATGA AGACTTTGGG CAGCCAGAGC TTCAACACAT CAGACTCCAC CATGCTCACT 
CCCCCTTCAA GCCCACCGCC ACCACCACCC CAAGACGAGG AACCCGCAAC TCTTCGGAGG 
AAGCCATATC AGACTCATGA GCATAAGGAC GCTGAGCCCA AGGCCTCGGT GATTCCACCA 
CCACCACCTG CGACGTTCAT GCGGGCCCCC CGGGAGCCCA CCCAGCCCCT CCAAGAGGAG 
ATGCGGAAGT CGTTTGTGGA GAGCGTGGAT GAGATCCCAT TTGCCGATGA TGTGGAGGAC 
ACCTATGATG ACAAGACCGA GGACTCGAGT CTCCAGGAGA AGTTCTTCAC ACCCCCCTCC 
TACTGGCCCC GCACGGAGAG GCCCCTCCAC CCACCCCTAG CCAAGGAGAA TGGTAGGTTG 
CCTACTCTGG AGAGTGGGGT CCAGCCACAG AAGAGGGGCC TGCCCTTAGT CTCTCCAGAA 
GCCAAGGAGC TGGCTGAGGA GCGCATGCGA GCCAGGGAGA AGTCTGTGAA GAGCCAGGCG 
TTGAGAGACG CTATGGCCAA GCAGCTGAGT AAGATGAAGG AAATGGAGAT GGCGGCTGGG 
ACCTCCAGTC CCCCAGGAGG CACCTCCCAC AAAGCCTCCT CTGTTCCCTC CAAGGGCAAA 
GACCTCAGCC CCGAGTCCCC TAGACGCCCA GTTCTCAAAG GCCCCAGGGA GCCTGCCCTG 
AAGCACGAAG CCACTAGTGA GGAGGTCCTC TCCCCACCAT CGGACTCAGG GGGCCCAGAT 
GGTTCGGTCA CCTCATCCGA GGGCTCCAGT GGGAAGAGCA AGAAGAGATC GTCACTCTTC 
TCCCCCCGCA GAAACAAGAA AGAGAAGAAG TCCAAAGGTG AGGGCCGGCC CCCGGAGAAG 
CCCAGCCCAA GCCTTCTGGA GGAAGCTGCT GCCAAGCCCA AGTCCCTGTG GAAGTCGGTC 
TTTTCCGGGT ACAAGAAGGA CAAAAAGAAG AAGGGTGATG ACAAGTCCTG TTCCAGCACC 
CCTTCCAGCG GTGCCACTGT GGACTCTGGC AAGCACAAGA TGTCCCCTGT TGTGAGAGCA 
GAGCTGCACC TGCGGCGCCA GTTGAGCTTC TCCGAGGACT CAGACCTGTC CAGTGATGAC 
ATCCTTGAGA GGTCCTCCCA GAAGTCCAGG CGAGAGTCGG TTTATGTACC ACACGCCTTG 
GCATTCAAGA GATCATATCC ATCAAAGCCG AGAACCTACA CAGAGGAGGA ACTGAATGCC 
AAGCTGACCC GGCGTGTGCA GAAGGCAGCT CGTAGACAGG CCAAGCAAGA GGAGCTGAAG 
CGGCTGCACC GAGCACAGAT CATCCAGAGG CAGCTGGAGC AGGTGGAGGA GAAGCAGAGA 
CAGCTGGAGG AAAGAGGGGT GGCCGTGGAG AAGGCGCTCC GTGGTGAAGC AGACTATTGG 
GGAGAGTCTT ATTACAGTGA CCTCATCGAC TTGCATCTGG GTGTTGAGCC CAGTGGCGGG 
ACTCCACGGC GTAGACCTCT CTCCTTCTGC CCTTGCTGTG TCCAGGAAGG CATGGGCAAG 
AAGGATGACC CCAAGCTGAT GCAGGAGTGG TTCAAGTTGG TGCAAGAGAA GAATGCCATG 
GTGCGCTATG AGTCGGAGCT GATGATTTTT GCCCGGGAGC TGGAACTGGA GGACCGGCAG 
AGCCGACTGC AGCAGGAGCT CCGGGAGCGG ATGGCGGTGG AAGATCACCT GAAGACTGAG 
GAGGAGCTGT CTGAGGAGAA GAAGATCTTG AACGAGATGC TGGAGGTGGT GGAGCAGAGA 
GATGCCCTCG TGGCCCTGCT TGAGGAGCAG CGACTCCGGG AGAAGGAGGA GGACAAAGAC 
CTGGAGGCTG TCATGCTGTC CAAGGGCTTC AGCCTGAACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963863.1
CDS	158..6985
Translation	MEDSKNETVNHAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDYRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKACTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFQGLVQPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELKEATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLNYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREQNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNININFLRPSQVRHLYNTGETRDIHLEMENLVNSRTTPKLTRNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGKEMASVGEPDKLSMVMYLTQFYEMFKDSLPSSDALDLNAEEKAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEEAPRGYRGGRPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSTGMRRQPTQERGVSQPSCCLPEQGHPAPTPQWKQQQEKERSRTCPKKVITLSPPLTPPPCRAPRSQQTYRDLDADSRGKQSPHHERPEPEPSRRFFVDQWELSLSLRSSNRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCRKRVYVMERLSAEGKFFHRSCFKCEYCATTLRLSAYAYDIEDGKFYCKPHYCYRLSGSAQRKRPAVAPLSGKEAREPLQDGPTVDTNGRASAVTSPAERTPGSSVNGVEEPSIAKRLRGTPERIELENYRLSVKQAEELEEVPEETQAEHNLSSVLDTGAEEDVASSSSESEMEEEEEEEPPLPTSDLGGVPWKEAVRIHALLKGKSEEELEASKSFGPGHEEDEEEEEYEEEEEEEEEEYEEEEEEEESSEEGEYCPWDRELLQGQWLRQLSQEETTGTRKVGSQRLQQIVNPADPLEIQADVHWTHIREREEEERMVPTSESSTSRVPDLPSIRRAAVQAWLETVSGVPFDENDLEEDVDSEPAKIEGEATENGDTGDTGAELDDDQRWSDGIPSDAETDLRVQHQEAVEADLELRVSENEEEPPEVTPGHPERSLTRVPSPALSPEEQAGLRSPGHSPKAEGAQIPLVSVVTKAKLPEENLFPKPLLPKVKPKVEVPCSQRAACSPIRSQPVALPEARTPTSPVSSQCLSPLATSTPTSTQLPICSQPQPSSETTAPSPTKSPIRLQPVSAKTSIPVVPLPLKNQGDTKDRLGSPLAVDEALKRNDLVAEFWMKSAEIRRSLGLTPVDRSKVYEPSFPSPAFKPVSLKSYAVEKSPEGEGLQLLKPPPVPKRLGLPKSDGDQPFLPTPKSPSDRELKSSQEERRDLSSSSGLGLHGSSSNMKTLGSQSFNTSDSTMLTPPSSPPPPPPQDEEPATLRRKPYQTHEHKDAEPKASVIPPPPPATFMRAPREPTQPLQEEMRKSFVESVDEIPFADDVEDTYDDKTEDSSLQEKFFTPPSYWPRTERPLHPPLAKENGRLPTLESGVQPQKRGLPLVSPEAKELAEERMRAREKSVKSQALRDAMAKQLSKMKEMEMAAGTSSPPGGTSHKASSVPSKGKDLSPESPRRPVLKGPREPALKHEATSEEVLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRNKKEKKSKGEGRPPEKPSPSLLEEAAAKPKSLWKSVFSGYKKDKKKKGDDKSCSSTPSSGATVDSGKHKMSPVVRAELHLRRQLSFSEDSDLSSDDILERSSQKSRRESVYVPHALAFKRSYPSKPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELSEEKKILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAVMLSKGFSLNWS

Target ORF information:

RefSeq Version	XM_014108388.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108388.1

ORF Insert Sequence:

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GCCGACACAA GAGCGTGGGG TCAGCCAGCC GTCCTGCTGC 
CTACCTGAGC AGGGTCACCC TGCTCCCACC CCCCAGTGGA AACAGCAACA GGAAAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACACTCTCTC CTCCCCTTAC TCCACCTCCC 
TGTCGGGCAC CTCGCAGCCA GCAGACGTAC AGGGATCTTG ATGCTGACAG CCGTGGCAAG 
CAGAGTCCCC ACCATGAGAG GCCAGAACCT GAACCTTCTC GTCGGTTTTT TGTTGACCAG 
TGGGAGCTTT CTCTTAGTCT CCGCTCCTCC AACCGCCCCG CCTCTCCCTC CTCTGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAGCCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCGATAAAGA AGGAGTTCCC ACAGAACTTA GGAGGCAGCG ACACGTGCTA TTTCTGCCGG 
AAGCGGGTCT ACGTGATGGA AAGGCTGAGT GCTGAGGGCA AGTTCTTCCA TCGGAGCTGC 
TTCAAGTGCG AGTACTGCGC CACCACCCTG CGCCTCTCGG CCTATGCCTA TGACATTGAG 
GACGGTAAAT TCTACTGCAA ACCACACTAC TGTTATCGAC TCTCTGGCTC TGCACAGAGG 
AAGAGACCAG CAGTGGCTCC TCTCTCTGGA AAGGAGGCCA GAGAACCCCT GCAGGATGGC 
CCCACTGTGG ACACAAATGG ACGGGCCAGC GCTGTCACCA GCCCTGCTGA GAGAACTCCA 
GGTTCAAGCG TGAATGGTGT GGAAGAGCCT AGCATTGCCA AGAGGCTCAG GGGCACTCCC 
GAGCGGATTG AGCTGGAGAA CTACCGCCTG TCCGTGAAGC AGGCCGAGGA GCTGGAGGAG 
GTGCCCGAGG AGACACAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGGGCAGAG 
GAGGACGTGG CCAGCAGCAG TTCTGAGTCA GAGATGGAGG AAGAGGAGGA AGAAGAGCCT 
CCTCTGCCCA CTTCGGACCT GGGCGGTGTT CCTTGGAAGG AGGCCGTGAG GATCCATGCT 
CTTCTGAAAG GAAAGAGTGA AGAGGAGCTA GAGGCCTCAA AGAGCTTTGG GCCTGGGCAT 
GAAGAAGACG AGGAGGAGGA GGAGTATGAA GAGGAGGAGG AGGAGGAGGA GGAAGAATAT 
GAAGAGGAAG AGGAAGAGGA AGAGTCTAGT GAAGAAGGGG AGTATTGCCC CTGGGACAGA 
GAACTCCTAC AAGGCCAGTG GCTCCGGCAG CTGTCACAGG AAGAAACTAC GGGTACACGT 
AAAGTTGGGA GCCAGAGGCT ACAGCAGATC GTAAATCCGG CCGACCCCTT GGAGATCCAG 
GCTGATGTGC ACTGGACACA TATCCGAGAG AGAGAGGAGG AGGAGCGAAT GGTCCCAACC 
TCTGAGTCCT CCACTTCTAG AGTGCCTGAC CTTCCCTCCA TACGCCGCGC AGCAGTGCAG 
GCCTGGCTGG AGACGGTCTC TGGAGTCCCA TTTGATGAGA ATGACCTGGA GGAAGACGTG 
GACTCGGAGC CAGCAAAGAT AGAAGGGGAG GCCACAGAGA ATGGGGACAC AGGGGACACT 
GGTGCTGAGC TGGACGATGA TCAGCGCTGG TCTGACGGGA TCCCATCGGA TGCCGAGACG 
GATCTCCGCG TGCAGCACCA GGAGGCGGTA GAGGCAGACC TGGAGCTGAG GGTCTCAGAG 
AATGAGGAGG AGCCACCTGA GGTCACACCA GGGCATCCGG AGAGAAGTCT CACCCGGGTC 
CCCAGCCCTG CCCTGTCCCC TGAGGAGCAA GCTGGTCTGC GGTCCCCAGG CCACAGCCCT 
AAGGCAGAGG GGGCCCAAAT CCCACTTGTC TCTGTTGTCA CCAAGGCAAA ATTGCCTGAA 
GAGAACCTTT TCCCCAAGCC TTTGCTCCCC AAAGTGAAGC CCAAGGTGGA AGTGCCCTGC 
AGTCAGAGAG CTGCATGCTC CCCCATCCGT TCTCAGCCAG TGGCTCTGCC AGAAGCCAGG 
ACACCTACCT CCCCAGTGAG CTCGCAGTGC CTGTCGCCTT TGGCCACCTC CACCCCCACC 
AGCACCCAGC TCCCCATTTG CTCCCAGCCT CAGCCTTCCT CTGAGACCAC GGCCCCATCC 
CCCACCAAGT CCCCCATACG GCTCCAGCCT GTTTCAGCCA AAACATCTAT CCCAGTGGTT 
CCCCTCCCCT TGAAGAACCA AGGGGACACC AAGGACAGGC TGGGCAGCCC TCTCGCTGTG 
GATGAGGCCC TGAAGCGGAA TGACCTGGTG GCAGAGTTCT GGATGAAGAG CGCCGAGATC 
CGCCGCAGCC TCGGGCTCAC ACCAGTGGAT CGGAGCAAGG TGTATGAGCC CAGCTTCCCT 
TCGCCTGCCT TTAAACCAGT GTCCCTAAAA TCCTATGCAG TGGAGAAGTC TCCCGAGGGG 
GAGGGACTCC AGCTTCTGAA GCCTCCACCT GTTCCTAAGA GACTAGGCCT GCCGAAGTCA 
GACGGTGACC AGCCCTTCCT GCCAACCCCC AAGTCCCCGT CGGACAGAGA GCTGAAAAGC 
TCCCAGGAGG AGAGGAGAGA TCTGTCGAGC AGCTCCGGGC TGGGCCTTCA CGGGAGCTCC 
TCCAACATGA AGACTTTGGG CAGCCAGAGC TTCAACACAT CAGACTCCAC CATGCTCACT 
CCCCCTTCAA GCCCACCGCC ACCACCACCC CAAGACGAGG AACCCGCAAC TCTTCGGAGG 
AAGCCATATC AGACTCATGA GCATAAGGAC GCTGAGCCCA AGGCCTCGGT GATTCCACCA 
CCACCACCTG CGACGTTCAT GCGGGCCCCC CGGGAGCCCA CCCAGCCCCT CCAAGAGGAG 
ATGCGGAAGT CGTTTGTGGA GAGCGTGGAT GAGATCCCAT TTGCCGATGA TGTGGAGGAC 
ACCTATGATG ACAAGACCGA GGACTCGAGT CTCCAGGAGA AGTTCTTCAC ACCCCCCTCC 
TACTGGCCCC GCACGGAGAG GCCCCTCCAC CCACCCCTAG CCAAGGAGAA TGGTAGGTTG 
CCTACTCTGG AGAGTGGGGT CCAGCCACAG AAGAGGGGCC TGCCCTTAGT CTCTCCAGAA 
GCCAAGGAGC TGGCTGAGGA GCGCATGCGA GCCAGGGAGA AGTCTGTGAA GAGCCAGGCG 
TTGAGAGACG CTATGGCCAA GCAGCTGAGT AAGATGAAGG AAATGGAGAT GGCGGCTGGG 
ACCTCCAGTC CCCCAGGAGG CACCTCCCAC AAAGCCTCCT CTGTTCCCTC CAAGGGCAAA 
GACCTCAGCC CCGAGTCCCC TAGACGCCCA GTTCTCAAAG GCCCCAGGGA GCCTGCCCTG 
AAGCACGAAG CCACTAGTGA GGAGGTCCTC TCCCCACCAT CGGACTCAGG GGGCCCAGAT 
GGTTCGGTCA CCTCATCCGA GGGCTCCAGT GGGAAGAGCA AGAAGAGATC GTCACTCTTC 
TCCCCCCGCA GAAACAAGAA AGAGAAGAAG TCCAAAGGTG AGGGCCGGCC CCCGGAGAAG 
CCCAGCCCAA GCCTTCTGGA GGAAGCTGCT GCCAAGCCCA AGTCCCTGTG GAAGTCGGTC 
TTTTCCGGGT ACAAGAAGGA CAAAAAGAAG AAGGGTGATG ACAAGTCCTG TTCCAGCACC 
CCTTCCAGCG GTGCCACTGT GGACTCTGGC AAGCACAAGA TGTCCCCTGT TGTGAGAGCA 
GAGCTGCACC TGCGGCGCCA GTTGAGCTTC TCCGAGGACT CAGACCTGTC CAGTGATGAC 
ATCCTTGAGA GGTCCTCCCA GAAGTCCAGG CGAGAGTCGG TTTATGTACC ACACGCCTTG 
GCATTCAAGA GATCATATCC ATCAAAGCCG AGAACCTACA CAGAGGAGGA ACTGAATGCC 
AAGCTGACCC GGCGTGTGCA GAAGGCAGCT CGTAGACAGG CCAAGCAAGA GGAGCTGAAG 
CGGCTGCACC GAGCACAGAT CATCCAGAGG CAGCTGGAGC AGGTGGAGGA GAAGCAGAGA 
CAGCTGGAGG AAAGAGGGGT GGCCGTGGAG AAGGCGCTCC GTGGTGAAGC AGACTATTGG 
GGAGAGTCTT ATTACAGTGA CCTCATCGAC TTGCATCTGG GTGTTGAGCC CAGTGGCGGG 
ACTCCACGGC GTAGACCTCT CTCCTTCTGC CCTTGCTGTG TCCAGGAAGG CATGGGCAAG 
AAGGATGACC CCAAGCTGAT GCAGGAGTGG TTCAAGTTGG TGCAAGAGAA GAATGCCATG 
GTGCGCTATG AGTCGGAGCT GATGATTTTT GCCCGGGAGC TGGAACTGGA GGACCGGCAG 
AGCCGACTGC AGCAGGAGCT CCGGGAGCGG ATGGCGGTGG AAGATCACCT GAAGACTGAG 
GAGGAGCTGT CTGAGGAGAA GAAGATCTTG AACGAGATGC TGGAGGTGGT GGAGCAGAGA 
GATGCCCTCG TGGCCCTGCT TGAGGAGCAG CGACTCCGGG AGAAGGAGGA GGACAAAGAC 
CTGGAGGCTG TCATGCTGTC CAAGGGCTTC AGCCTGAACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_022410894.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022266602.1 Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X25, mRNA.

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CloneID	OCe43614
Clone ID Related Accession (Same CDS sequence)	XM_022410894.1 , XM_014108389.1
Accession Version	XM_022410894.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	F-actin-methionine sulfoxide oxidase MICAL3 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GGGCTCGATA AAGAAGGAGT TCCCACAGAA CTTAGGAGGC 
AGCGACACGT GCTATTTCTG CCGGAAGCGG GTCTACGTGA TGGAAAGGCT GAGTGCTGAG 
GGCAAGTTCT TCCATCGGAG CTGCTTCAAG TGCGAGTACT GCGCCACCAC CCTGCGCCTC 
TCGGCCTATG CCTATGACAT TGAGGACGGT AAATTCTACT GCAAACCACA CTACTGTTAT 
CGACTCTCTG GCTCTGCACA GAGGAAGAGA CCAGCAGTGG CTCCTCTCTC TGGAAAGGAG 
GCCAGAGAAC CCCTGCAGGA TGGCCCCACT GTGGACACAA ATGGACGGGC CAGCGCTGTC 
ACCAGCCCTG CTGAGAGAAC TCCAGGTTCA AGCGTGAATG GTGTGGAAGA GCCTAGCATT 
GCCAAGAGGC TCAGGGGCAC TCCCGAGCGG ATTGAGCTGG AGAACTACCG CCTGTCCGTG 
AAGCAGGCCG AGGAGCTGGA GGAGGTGCCC GAGGAGACAC AGGCCGAGCA CAACCTGAGC 
AGCGTGCTGG ACACGGGGGC AGAGGAGGAC GTGGCCAGCA GCAGTTCTGA GTCAGAGATG 
GAGGAAGAGG AGGAAGAAGA GCCTCCTCTG CCCACTTCGG ACCTGGGCGG TGTTCCTTGG 
AAGGAGGCCG TGAGGATCCA TGCTCTTCTG AAAGGAAAGA GTGAAGAGGA GCTAGAGGCC 
TCAAAGAGCT TTGGGCCTGG GCATGAAGAA GACGAGGAGG AGGAGGAGTA TGAAGAGGAG 
GAGGAGGAGG AGGAGGAAGA ATATGAAGAG GAAGAGGAAG AGGAAGAGTC TAGTGAAGTT 
GGGAGCCAGA GGCTACAGCA GATCGTAAAT CCGGCCGACC CCTTGGAGAT CCAGGCTGAT 
GTGCACTGGA CACATATCCG AGAGAGAGAG GAGGAGGAGC GAATGGTCCC AACCTCTGAG 
TCCTCCACTT CTAGAGTCCC ATTTGATGAG AATGACCTGG AGGAAGACGT GGACTCGGAG 
CCAGCAAAGA TAGAAGGGGA GGCCACAGAG AATGGGGACA CAGGGGACAC TGGTGCTGAG 
CTGGACGATG ATCAGCGCTG GTCTGACGGG ATCCCATCGG ATGCCGAGAC GGATCTCCGC 
GTGCAGCACC AGGAGGCGGT AGAGGCAGAC CTGGAGCTGA GGGTCTCAGA GAATGAGGAG 
GAGCCACCTG AGGTCACACC AGGGCATCCG GAGAGAAGTC TCACCCGGGT CCCCAGCCCT 
GCCCTGTCCC CTGAGGAGCA AGCTGGTCTG CGGTCCCCAG GCCACAGCCC TAAGGCAGAG 
GGGGCCCAAA TCCCACTTGT CTCTGTTGTC ACCAAGGCAA AATTGCCTGA AGAGAACCTT 
TTCCCCAAGC CTTTGCTCCC CAAAGTGAAG CCCAAGGTGG AAGTGCCCTG CAGTCAGAGA 
GCTGCATGCT CCCCCATCCG TTCTCAGCCA GTGGCTCTGC CAGAAGCCAG GACACCTACC 
TCCCCAGTGA GCTCGCAGTG CCTGTCGCCT TTGGCCACCT CCACCCCCAC CAGCACCCAG 
CTCCCCATTT GCTCCCAGCC TCAGCCTTCC TCTGAGACCA CGGCCCCATC CCCCACCAAG 
TCCCCCATAC GGCTCCAGCC TGTTTCAGCC AAAACATCTA TCCCAGTGGT TCCCCTCCCC 
TTGAAGAACC AAGGGGACAC CAAGGACAGG CTGGGCAGCC CTCTCGCTGT GGATGAGGCC 
CTGAAGCGGA ATGACCTGGT GGCAGAGTTC TGGATGAAGA GCGCCGAGAT CCGCCGCAGC 
CTCGGGCTCA CACCAGTGGA TCGGAGCAAG GTGTATGAGC CCAGCTTCCC TTCGCCTGCC 
TTTAAACCAG TGTCCCTAAA ATCCTATGCA GTGGAGAAGT CTCCCGAGGG GGAGGGACTC 
CAGCTTCTGA AGCCTCCACC TGTTCCTAAG AGACTAGGCC TGCCGAAGTC AGACGGTGAC 
CAGCCCTTCC TGCCAACCCC CAAGTCCCCG TCGGACAGAG AGCTGAAAAG CTCCCAGGAG 
GAGAGGAGAG ATCTGTCGAG CAGCTCCGGG CTGGGCCTTC ACGGGAGCTC CTCCAACATG 
AAGACTTTGG GCAGCCAGAG CTTCAACACA TCAGACTCCA CCATGCTCAC TCCCCCTTCA 
AGCCCACCGC CACCACCACC CCAAGACGAG GAACCCGCAA CTCTTCGGAG GAAGCCATAT 
CAGACTCATG AGCATAAGGA CGCTGAGCCC AAGGCCTCGG TGATTCCACC ACCACCACCT 
GCGACGTTCA TGCGGGCCCC CCGGGAGCCC ACCCAGCCCC TCCAAGAGGA GATGCGGAAG 
TCGTTTGTGG AGAGCGTGGA TGAGATCCCA TTTGCCGATG ATGTGGAGGA CACCTATGAT 
GACAAGACCG AGGACTCGAG TCTCCAGGAG AAGTTCTTCA CACCCCCCTC CTACTGGCCC 
CGCACGGAGA GGCCCCTCCA CCCACCCCTA GCCAAGGAGA ATGGTAGGTT GCCTACTCTG 
GAGAGTGGGG TCCAGCCACA GAAGAGGGGC CTGCCCTTAG TCTCTCCAGA AGCCAAGGAG 
CTGGCTGAGG AGCGCATGCG AGCCAGGGAG AAGTCTGTGA AGAGCCAGGC GTTGAGAGAC 
GCTATGGCCA AGCAGCTGAG TAAGATGAAG GAAATGGAGA TGGCGGCTGG GACCTCCAGT 
CCCCCAGGAG GCACCTCCCA CAAAGCCTCC TCTGTTCCCT CCAAGGGCAA AGACCTCAGC 
CCCGAGTCCC CTAGACGCCC AGTTCTCAAA GGCCCCAGGG AGCCTGCCCT GAAGCACGAA 
GCCACTAGTG AGGAGGTCCT CTCCCCACCA TCGGACTCAG GGGGCCCAGA TGGTTCGGTC 
ACCTCATCCG AGGGCTCCAG TGGGAAGAGC AAGAAGAGAT CGTCACTCTT CTCCCCCCGC 
AGAAACAAGA AAGAGAAGAA GTCCAAAGGT GAGGGCCGGC CCCCGGAGAA GCCCAGCCCA 
AGCCTTCTGG AGGAAGCTGC TGCCAAGCCC AAGTCCCTGT GGAAGTCGGT CTTTTCCGGG 
TACAAGAAGG ACAAAAAGAA GAAGGGTGAT GACAAGTCCT GTTCCAGCAC CCCTTCCAGC 
GGTGCCACTG TGGACTCTGG CAAGCACAAG ATGTCCCCTG TTGTGAGAGC AGAGCTGCAC 
CTGCGGCGCC AGTTGAGCTT CTCCGAGGAC TCAGACCTGT CCAGTGATGA CATCCTTGAG 
AGGTCCTCCC AGAAGTCCAG GCGAGAGTCG GTTTATGTAC CACACGCCTT GGCATTCAAG 
AGATCATATC CATCAAAGCC GAGAACCTAC ACAGAGGAGG AACTGAATGC CAAGCTGACC 
CGGCGTGTGC AGAAGGCAGC TCGTAGACAG GCCAAGCAAG AGGAGCTGAA GCGGCTGCAC 
CGAGCACAGA TCATCCAGAG GCAGCTGGAG CAGGTGGAGG AGAAGCAGAG ACAGCTGGAG 
GAAAGAGGGG TGGCCGTGGA GAAGGCGCTC CGTGGTGAAG CAGACTATTG GGGAGAGTCT 
TATTACAGTG ACCTCATCGA CTTGCATCTG GGTGTTGAGC CCAGTGGCGG GACTCCACGG 
CGTAGACCTC TCTCCTTCTG CCCTTGCTGT GTCCAGGAAG GCATGGGCAA GAAGGATGAC 
CCCAAGCTGA TGCAGGAGTG GTTCAAGTTG GTGCAAGAGA AGAATGCCAT GGTGCGCTAT 
GAGTCGGAGC TGATGATTTT TGCCCGGGAG CTGGAACTGG AGGACCGGCA GAGCCGACTG 
CAGCAGGAGC TCCGGGAGCG GATGGCGGTG GAAGATCACC TGAAGACTGA GGAGGAGCTG 
TCTGAGGAGA AGAAGATCTT GAACGAGATG CTGGAGGTGG TGGAGCAGAG AGATGCCCTC 
GTGGCCCTGC TTGAGGAGCA GCGACTCCGG GAGAAGGAGG AGGACAAAGA CCTGGAGGCT 
GTCATGCTGT CCAAGGGCTT CAGCCTGAAC TGGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022266602.1
CDS	257..6475
Translation	MEDSKNETVNHAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDYRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKACTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFQGLVQPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELKEATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLNYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREQNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNININFLRPSQVRHLYNTGETRDIHLEMENLVNSRTTPKLTRNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGKEMASVGEPDKLSMVMYLTQFYEMFKDSLPSSDALDLNAEEKAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEEAPRGYRGGRPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSTGMRRQGSIKKEFPQNLGGSDTCYFCRKRVYVMERLSAEGKFFHRSCFKCEYCATTLRLSAYAYDIEDGKFYCKPHYCYRLSGSAQRKRPAVAPLSGKEAREPLQDGPTVDTNGRASAVTSPAERTPGSSVNGVEEPSIAKRLRGTPERIELENYRLSVKQAEELEEVPEETQAEHNLSSVLDTGAEEDVASSSSESEMEEEEEEEPPLPTSDLGGVPWKEAVRIHALLKGKSEEELEASKSFGPGHEEDEEEEEYEEEEEEEEEEYEEEEEEEESSEVGSQRLQQIVNPADPLEIQADVHWTHIREREEEERMVPTSESSTSRVPFDENDLEEDVDSEPAKIEGEATENGDTGDTGAELDDDQRWSDGIPSDAETDLRVQHQEAVEADLELRVSENEEEPPEVTPGHPERSLTRVPSPALSPEEQAGLRSPGHSPKAEGAQIPLVSVVTKAKLPEENLFPKPLLPKVKPKVEVPCSQRAACSPIRSQPVALPEARTPTSPVSSQCLSPLATSTPTSTQLPICSQPQPSSETTAPSPTKSPIRLQPVSAKTSIPVVPLPLKNQGDTKDRLGSPLAVDEALKRNDLVAEFWMKSAEIRRSLGLTPVDRSKVYEPSFPSPAFKPVSLKSYAVEKSPEGEGLQLLKPPPVPKRLGLPKSDGDQPFLPTPKSPSDRELKSSQEERRDLSSSSGLGLHGSSSNMKTLGSQSFNTSDSTMLTPPSSPPPPPPQDEEPATLRRKPYQTHEHKDAEPKASVIPPPPPATFMRAPREPTQPLQEEMRKSFVESVDEIPFADDVEDTYDDKTEDSSLQEKFFTPPSYWPRTERPLHPPLAKENGRLPTLESGVQPQKRGLPLVSPEAKELAEERMRAREKSVKSQALRDAMAKQLSKMKEMEMAAGTSSPPGGTSHKASSVPSKGKDLSPESPRRPVLKGPREPALKHEATSEEVLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRNKKEKKSKGEGRPPEKPSPSLLEEAAAKPKSLWKSVFSGYKKDKKKKGDDKSCSSTPSSGATVDSGKHKMSPVVRAELHLRRQLSFSEDSDLSSDDILERSSQKSRRESVYVPHALAFKRSYPSKPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELSEEKKILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAVMLSKGFSLNWS

Target ORF information:

RefSeq Version	XM_022410894.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022410894.1

ORF Insert Sequence:

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GGGCTCGATA AAGAAGGAGT TCCCACAGAA CTTAGGAGGC 
AGCGACACGT GCTATTTCTG CCGGAAGCGG GTCTACGTGA TGGAAAGGCT GAGTGCTGAG 
GGCAAGTTCT TCCATCGGAG CTGCTTCAAG TGCGAGTACT GCGCCACCAC CCTGCGCCTC 
TCGGCCTATG CCTATGACAT TGAGGACGGT AAATTCTACT GCAAACCACA CTACTGTTAT 
CGACTCTCTG GCTCTGCACA GAGGAAGAGA CCAGCAGTGG CTCCTCTCTC TGGAAAGGAG 
GCCAGAGAAC CCCTGCAGGA TGGCCCCACT GTGGACACAA ATGGACGGGC CAGCGCTGTC 
ACCAGCCCTG CTGAGAGAAC TCCAGGTTCA AGCGTGAATG GTGTGGAAGA GCCTAGCATT 
GCCAAGAGGC TCAGGGGCAC TCCCGAGCGG ATTGAGCTGG AGAACTACCG CCTGTCCGTG 
AAGCAGGCCG AGGAGCTGGA GGAGGTGCCC GAGGAGACAC AGGCCGAGCA CAACCTGAGC 
AGCGTGCTGG ACACGGGGGC AGAGGAGGAC GTGGCCAGCA GCAGTTCTGA GTCAGAGATG 
GAGGAAGAGG AGGAAGAAGA GCCTCCTCTG CCCACTTCGG ACCTGGGCGG TGTTCCTTGG 
AAGGAGGCCG TGAGGATCCA TGCTCTTCTG AAAGGAAAGA GTGAAGAGGA GCTAGAGGCC 
TCAAAGAGCT TTGGGCCTGG GCATGAAGAA GACGAGGAGG AGGAGGAGTA TGAAGAGGAG 
GAGGAGGAGG AGGAGGAAGA ATATGAAGAG GAAGAGGAAG AGGAAGAGTC TAGTGAAGTT 
GGGAGCCAGA GGCTACAGCA GATCGTAAAT CCGGCCGACC CCTTGGAGAT CCAGGCTGAT 
GTGCACTGGA CACATATCCG AGAGAGAGAG GAGGAGGAGC GAATGGTCCC AACCTCTGAG 
TCCTCCACTT CTAGAGTCCC ATTTGATGAG AATGACCTGG AGGAAGACGT GGACTCGGAG 
CCAGCAAAGA TAGAAGGGGA GGCCACAGAG AATGGGGACA CAGGGGACAC TGGTGCTGAG 
CTGGACGATG ATCAGCGCTG GTCTGACGGG ATCCCATCGG ATGCCGAGAC GGATCTCCGC 
GTGCAGCACC AGGAGGCGGT AGAGGCAGAC CTGGAGCTGA GGGTCTCAGA GAATGAGGAG 
GAGCCACCTG AGGTCACACC AGGGCATCCG GAGAGAAGTC TCACCCGGGT CCCCAGCCCT 
GCCCTGTCCC CTGAGGAGCA AGCTGGTCTG CGGTCCCCAG GCCACAGCCC TAAGGCAGAG 
GGGGCCCAAA TCCCACTTGT CTCTGTTGTC ACCAAGGCAA AATTGCCTGA AGAGAACCTT 
TTCCCCAAGC CTTTGCTCCC CAAAGTGAAG CCCAAGGTGG AAGTGCCCTG CAGTCAGAGA 
GCTGCATGCT CCCCCATCCG TTCTCAGCCA GTGGCTCTGC CAGAAGCCAG GACACCTACC 
TCCCCAGTGA GCTCGCAGTG CCTGTCGCCT TTGGCCACCT CCACCCCCAC CAGCACCCAG 
CTCCCCATTT GCTCCCAGCC TCAGCCTTCC TCTGAGACCA CGGCCCCATC CCCCACCAAG 
TCCCCCATAC GGCTCCAGCC TGTTTCAGCC AAAACATCTA TCCCAGTGGT TCCCCTCCCC 
TTGAAGAACC AAGGGGACAC CAAGGACAGG CTGGGCAGCC CTCTCGCTGT GGATGAGGCC 
CTGAAGCGGA ATGACCTGGT GGCAGAGTTC TGGATGAAGA GCGCCGAGAT CCGCCGCAGC 
CTCGGGCTCA CACCAGTGGA TCGGAGCAAG GTGTATGAGC CCAGCTTCCC TTCGCCTGCC 
TTTAAACCAG TGTCCCTAAA ATCCTATGCA GTGGAGAAGT CTCCCGAGGG GGAGGGACTC 
CAGCTTCTGA AGCCTCCACC TGTTCCTAAG AGACTAGGCC TGCCGAAGTC AGACGGTGAC 
CAGCCCTTCC TGCCAACCCC CAAGTCCCCG TCGGACAGAG AGCTGAAAAG CTCCCAGGAG 
GAGAGGAGAG ATCTGTCGAG CAGCTCCGGG CTGGGCCTTC ACGGGAGCTC CTCCAACATG 
AAGACTTTGG GCAGCCAGAG CTTCAACACA TCAGACTCCA CCATGCTCAC TCCCCCTTCA 
AGCCCACCGC CACCACCACC CCAAGACGAG GAACCCGCAA CTCTTCGGAG GAAGCCATAT 
CAGACTCATG AGCATAAGGA CGCTGAGCCC AAGGCCTCGG TGATTCCACC ACCACCACCT 
GCGACGTTCA TGCGGGCCCC CCGGGAGCCC ACCCAGCCCC TCCAAGAGGA GATGCGGAAG 
TCGTTTGTGG AGAGCGTGGA TGAGATCCCA TTTGCCGATG ATGTGGAGGA CACCTATGAT 
GACAAGACCG AGGACTCGAG TCTCCAGGAG AAGTTCTTCA CACCCCCCTC CTACTGGCCC 
CGCACGGAGA GGCCCCTCCA CCCACCCCTA GCCAAGGAGA ATGGTAGGTT GCCTACTCTG 
GAGAGTGGGG TCCAGCCACA GAAGAGGGGC CTGCCCTTAG TCTCTCCAGA AGCCAAGGAG 
CTGGCTGAGG AGCGCATGCG AGCCAGGGAG AAGTCTGTGA AGAGCCAGGC GTTGAGAGAC 
GCTATGGCCA AGCAGCTGAG TAAGATGAAG GAAATGGAGA TGGCGGCTGG GACCTCCAGT 
CCCCCAGGAG GCACCTCCCA CAAAGCCTCC TCTGTTCCCT CCAAGGGCAA AGACCTCAGC 
CCCGAGTCCC CTAGACGCCC AGTTCTCAAA GGCCCCAGGG AGCCTGCCCT GAAGCACGAA 
GCCACTAGTG AGGAGGTCCT CTCCCCACCA TCGGACTCAG GGGGCCCAGA TGGTTCGGTC 
ACCTCATCCG AGGGCTCCAG TGGGAAGAGC AAGAAGAGAT CGTCACTCTT CTCCCCCCGC 
AGAAACAAGA AAGAGAAGAA GTCCAAAGGT GAGGGCCGGC CCCCGGAGAA GCCCAGCCCA 
AGCCTTCTGG AGGAAGCTGC TGCCAAGCCC AAGTCCCTGT GGAAGTCGGT CTTTTCCGGG 
TACAAGAAGG ACAAAAAGAA GAAGGGTGAT GACAAGTCCT GTTCCAGCAC CCCTTCCAGC 
GGTGCCACTG TGGACTCTGG CAAGCACAAG ATGTCCCCTG TTGTGAGAGC AGAGCTGCAC 
CTGCGGCGCC AGTTGAGCTT CTCCGAGGAC TCAGACCTGT CCAGTGATGA CATCCTTGAG 
AGGTCCTCCC AGAAGTCCAG GCGAGAGTCG GTTTATGTAC CACACGCCTT GGCATTCAAG 
AGATCATATC CATCAAAGCC GAGAACCTAC ACAGAGGAGG AACTGAATGC CAAGCTGACC 
CGGCGTGTGC AGAAGGCAGC TCGTAGACAG GCCAAGCAAG AGGAGCTGAA GCGGCTGCAC 
CGAGCACAGA TCATCCAGAG GCAGCTGGAG CAGGTGGAGG AGAAGCAGAG ACAGCTGGAG 
GAAAGAGGGG TGGCCGTGGA GAAGGCGCTC CGTGGTGAAG CAGACTATTG GGGAGAGTCT 
TATTACAGTG ACCTCATCGA CTTGCATCTG GGTGTTGAGC CCAGTGGCGG GACTCCACGG 
CGTAGACCTC TCTCCTTCTG CCCTTGCTGT GTCCAGGAAG GCATGGGCAA GAAGGATGAC 
CCCAAGCTGA TGCAGGAGTG GTTCAAGTTG GTGCAAGAGA AGAATGCCAT GGTGCGCTAT 
GAGTCGGAGC TGATGATTTT TGCCCGGGAG CTGGAACTGG AGGACCGGCA GAGCCGACTG 
CAGCAGGAGC TCCGGGAGCG GATGGCGGTG GAAGATCACC TGAAGACTGA GGAGGAGCTG 
TCTGAGGAGA AGAAGATCTT GAACGAGATG CTGGAGGTGG TGGAGCAGAG AGATGCCCTC 
GTGGCCCTGC TTGAGGAGCA GCGACTCCGG GAGAAGGAGG AGGACAAAGA CCTGGAGGCT 
GTCATGCTGT CCAAGGGCTT CAGCCTGAAC TGGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_014108389.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963864.1 Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X7, mRNA.

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CloneID	OCe43614
Clone ID Related Accession (Same CDS sequence)	XM_022410894.1 , XM_014108389.1
Accession Version	XM_014108389.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GGGCTCGATA AAGAAGGAGT TCCCACAGAA CTTAGGAGGC 
AGCGACACGT GCTATTTCTG CCGGAAGCGG GTCTACGTGA TGGAAAGGCT GAGTGCTGAG 
GGCAAGTTCT TCCATCGGAG CTGCTTCAAG TGCGAGTACT GCGCCACCAC CCTGCGCCTC 
TCGGCCTATG CCTATGACAT TGAGGACGGT AAATTCTACT GCAAACCACA CTACTGTTAT 
CGACTCTCTG GCTCTGCACA GAGGAAGAGA CCAGCAGTGG CTCCTCTCTC TGGAAAGGAG 
GCCAGAGAAC CCCTGCAGGA TGGCCCCACT GTGGACACAA ATGGACGGGC CAGCGCTGTC 
ACCAGCCCTG CTGAGAGAAC TCCAGGTTCA AGCGTGAATG GTGTGGAAGA GCCTAGCATT 
GCCAAGAGGC TCAGGGGCAC TCCCGAGCGG ATTGAGCTGG AGAACTACCG CCTGTCCGTG 
AAGCAGGCCG AGGAGCTGGA GGAGGTGCCC GAGGAGACAC AGGCCGAGCA CAACCTGAGC 
AGCGTGCTGG ACACGGGGGC AGAGGAGGAC GTGGCCAGCA GCAGTTCTGA GTCAGAGATG 
GAGGAAGAGG AGGAAGAAGA GCCTCCTCTG CCCACTTCGG ACCTGGGCGG TGTTCCTTGG 
AAGGAGGCCG TGAGGATCCA TGCTCTTCTG AAAGGAAAGA GTGAAGAGGA GCTAGAGGCC 
TCAAAGAGCT TTGGGCCTGG GCATGAAGAA GACGAGGAGG AGGAGGAGTA TGAAGAGGAG 
GAGGAGGAGG AGGAGGAAGA ATATGAAGAG GAAGAGGAAG AGGAAGAGTC TAGTGAAGTT 
GGGAGCCAGA GGCTACAGCA GATCGTAAAT CCGGCCGACC CCTTGGAGAT CCAGGCTGAT 
GTGCACTGGA CACATATCCG AGAGAGAGAG GAGGAGGAGC GAATGGTCCC AACCTCTGAG 
TCCTCCACTT CTAGAGTCCC ATTTGATGAG AATGACCTGG AGGAAGACGT GGACTCGGAG 
CCAGCAAAGA TAGAAGGGGA GGCCACAGAG AATGGGGACA CAGGGGACAC TGGTGCTGAG 
CTGGACGATG ATCAGCGCTG GTCTGACGGG ATCCCATCGG ATGCCGAGAC GGATCTCCGC 
GTGCAGCACC AGGAGGCGGT AGAGGCAGAC CTGGAGCTGA GGGTCTCAGA GAATGAGGAG 
GAGCCACCTG AGGTCACACC AGGGCATCCG GAGAGAAGTC TCACCCGGGT CCCCAGCCCT 
GCCCTGTCCC CTGAGGAGCA AGCTGGTCTG CGGTCCCCAG GCCACAGCCC TAAGGCAGAG 
GGGGCCCAAA TCCCACTTGT CTCTGTTGTC ACCAAGGCAA AATTGCCTGA AGAGAACCTT 
TTCCCCAAGC CTTTGCTCCC CAAAGTGAAG CCCAAGGTGG AAGTGCCCTG CAGTCAGAGA 
GCTGCATGCT CCCCCATCCG TTCTCAGCCA GTGGCTCTGC CAGAAGCCAG GACACCTACC 
TCCCCAGTGA GCTCGCAGTG CCTGTCGCCT TTGGCCACCT CCACCCCCAC CAGCACCCAG 
CTCCCCATTT GCTCCCAGCC TCAGCCTTCC TCTGAGACCA CGGCCCCATC CCCCACCAAG 
TCCCCCATAC GGCTCCAGCC TGTTTCAGCC AAAACATCTA TCCCAGTGGT TCCCCTCCCC 
TTGAAGAACC AAGGGGACAC CAAGGACAGG CTGGGCAGCC CTCTCGCTGT GGATGAGGCC 
CTGAAGCGGA ATGACCTGGT GGCAGAGTTC TGGATGAAGA GCGCCGAGAT CCGCCGCAGC 
CTCGGGCTCA CACCAGTGGA TCGGAGCAAG GTGTATGAGC CCAGCTTCCC TTCGCCTGCC 
TTTAAACCAG TGTCCCTAAA ATCCTATGCA GTGGAGAAGT CTCCCGAGGG GGAGGGACTC 
CAGCTTCTGA AGCCTCCACC TGTTCCTAAG AGACTAGGCC TGCCGAAGTC AGACGGTGAC 
CAGCCCTTCC TGCCAACCCC CAAGTCCCCG TCGGACAGAG AGCTGAAAAG CTCCCAGGAG 
GAGAGGAGAG ATCTGTCGAG CAGCTCCGGG CTGGGCCTTC ACGGGAGCTC CTCCAACATG 
AAGACTTTGG GCAGCCAGAG CTTCAACACA TCAGACTCCA CCATGCTCAC TCCCCCTTCA 
AGCCCACCGC CACCACCACC CCAAGACGAG GAACCCGCAA CTCTTCGGAG GAAGCCATAT 
CAGACTCATG AGCATAAGGA CGCTGAGCCC AAGGCCTCGG TGATTCCACC ACCACCACCT 
GCGACGTTCA TGCGGGCCCC CCGGGAGCCC ACCCAGCCCC TCCAAGAGGA GATGCGGAAG 
TCGTTTGTGG AGAGCGTGGA TGAGATCCCA TTTGCCGATG ATGTGGAGGA CACCTATGAT 
GACAAGACCG AGGACTCGAG TCTCCAGGAG AAGTTCTTCA CACCCCCCTC CTACTGGCCC 
CGCACGGAGA GGCCCCTCCA CCCACCCCTA GCCAAGGAGA ATGGTAGGTT GCCTACTCTG 
GAGAGTGGGG TCCAGCCACA GAAGAGGGGC CTGCCCTTAG TCTCTCCAGA AGCCAAGGAG 
CTGGCTGAGG AGCGCATGCG AGCCAGGGAG AAGTCTGTGA AGAGCCAGGC GTTGAGAGAC 
GCTATGGCCA AGCAGCTGAG TAAGATGAAG GAAATGGAGA TGGCGGCTGG GACCTCCAGT 
CCCCCAGGAG GCACCTCCCA CAAAGCCTCC TCTGTTCCCT CCAAGGGCAA AGACCTCAGC 
CCCGAGTCCC CTAGACGCCC AGTTCTCAAA GGCCCCAGGG AGCCTGCCCT GAAGCACGAA 
GCCACTAGTG AGGAGGTCCT CTCCCCACCA TCGGACTCAG GGGGCCCAGA TGGTTCGGTC 
ACCTCATCCG AGGGCTCCAG TGGGAAGAGC AAGAAGAGAT CGTCACTCTT CTCCCCCCGC 
AGAAACAAGA AAGAGAAGAA GTCCAAAGGT GAGGGCCGGC CCCCGGAGAA GCCCAGCCCA 
AGCCTTCTGG AGGAAGCTGC TGCCAAGCCC AAGTCCCTGT GGAAGTCGGT CTTTTCCGGG 
TACAAGAAGG ACAAAAAGAA GAAGGGTGAT GACAAGTCCT GTTCCAGCAC CCCTTCCAGC 
GGTGCCACTG TGGACTCTGG CAAGCACAAG ATGTCCCCTG TTGTGAGAGC AGAGCTGCAC 
CTGCGGCGCC AGTTGAGCTT CTCCGAGGAC TCAGACCTGT CCAGTGATGA CATCCTTGAG 
AGGTCCTCCC AGAAGTCCAG GCGAGAGTCG GTTTATGTAC CACACGCCTT GGCATTCAAG 
AGATCATATC CATCAAAGCC GAGAACCTAC ACAGAGGAGG AACTGAATGC CAAGCTGACC 
CGGCGTGTGC AGAAGGCAGC TCGTAGACAG GCCAAGCAAG AGGAGCTGAA GCGGCTGCAC 
CGAGCACAGA TCATCCAGAG GCAGCTGGAG CAGGTGGAGG AGAAGCAGAG ACAGCTGGAG 
GAAAGAGGGG TGGCCGTGGA GAAGGCGCTC CGTGGTGAAG CAGACTATTG GGGAGAGTCT 
TATTACAGTG ACCTCATCGA CTTGCATCTG GGTGTTGAGC CCAGTGGCGG GACTCCACGG 
CGTAGACCTC TCTCCTTCTG CCCTTGCTGT GTCCAGGAAG GCATGGGCAA GAAGGATGAC 
CCCAAGCTGA TGCAGGAGTG GTTCAAGTTG GTGCAAGAGA AGAATGCCAT GGTGCGCTAT 
GAGTCGGAGC TGATGATTTT TGCCCGGGAG CTGGAACTGG AGGACCGGCA GAGCCGACTG 
CAGCAGGAGC TCCGGGAGCG GATGGCGGTG GAAGATCACC TGAAGACTGA GGAGGAGCTG 
TCTGAGGAGA AGAAGATCTT GAACGAGATG CTGGAGGTGG TGGAGCAGAG AGATGCCCTC 
GTGGCCCTGC TTGAGGAGCA GCGACTCCGG GAGAAGGAGG AGGACAAAGA CCTGGAGGCT 
GTCATGCTGT CCAAGGGCTT CAGCCTGAAC TGGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963864.1
CDS	1092..7310
Translation	MEDSKNETVNHAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDYRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKACTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFQGLVQPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELKEATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLNYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREQNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNININFLRPSQVRHLYNTGETRDIHLEMENLVNSRTTPKLTRNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGKEMASVGEPDKLSMVMYLTQFYEMFKDSLPSSDALDLNAEEKAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEEAPRGYRGGRPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSTGMRRQGSIKKEFPQNLGGSDTCYFCRKRVYVMERLSAEGKFFHRSCFKCEYCATTLRLSAYAYDIEDGKFYCKPHYCYRLSGSAQRKRPAVAPLSGKEAREPLQDGPTVDTNGRASAVTSPAERTPGSSVNGVEEPSIAKRLRGTPERIELENYRLSVKQAEELEEVPEETQAEHNLSSVLDTGAEEDVASSSSESEMEEEEEEEPPLPTSDLGGVPWKEAVRIHALLKGKSEEELEASKSFGPGHEEDEEEEEYEEEEEEEEEEYEEEEEEEESSEVGSQRLQQIVNPADPLEIQADVHWTHIREREEEERMVPTSESSTSRVPFDENDLEEDVDSEPAKIEGEATENGDTGDTGAELDDDQRWSDGIPSDAETDLRVQHQEAVEADLELRVSENEEEPPEVTPGHPERSLTRVPSPALSPEEQAGLRSPGHSPKAEGAQIPLVSVVTKAKLPEENLFPKPLLPKVKPKVEVPCSQRAACSPIRSQPVALPEARTPTSPVSSQCLSPLATSTPTSTQLPICSQPQPSSETTAPSPTKSPIRLQPVSAKTSIPVVPLPLKNQGDTKDRLGSPLAVDEALKRNDLVAEFWMKSAEIRRSLGLTPVDRSKVYEPSFPSPAFKPVSLKSYAVEKSPEGEGLQLLKPPPVPKRLGLPKSDGDQPFLPTPKSPSDRELKSSQEERRDLSSSSGLGLHGSSSNMKTLGSQSFNTSDSTMLTPPSSPPPPPPQDEEPATLRRKPYQTHEHKDAEPKASVIPPPPPATFMRAPREPTQPLQEEMRKSFVESVDEIPFADDVEDTYDDKTEDSSLQEKFFTPPSYWPRTERPLHPPLAKENGRLPTLESGVQPQKRGLPLVSPEAKELAEERMRAREKSVKSQALRDAMAKQLSKMKEMEMAAGTSSPPGGTSHKASSVPSKGKDLSPESPRRPVLKGPREPALKHEATSEEVLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRNKKEKKSKGEGRPPEKPSPSLLEEAAAKPKSLWKSVFSGYKKDKKKKGDDKSCSSTPSSGATVDSGKHKMSPVVRAELHLRRQLSFSEDSDLSSDDILERSSQKSRRESVYVPHALAFKRSYPSKPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELSEEKKILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAVMLSKGFSLNWS

Target ORF information:

RefSeq Version	XM_014108389.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108389.1

ORF Insert Sequence:

ATGGAGGACA GTAAGAATGA GACGGTGAAT CATGCTCACG TCCTCTTTGA CCGGTTTGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCTTTCCAAG AGCTCTGTGA CCACCTCGAA 
CTGAAGCCTA AGGACTACCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAAG CTCTGTGGGC AAAGTTGGAC AAGCGGGGCA GCCACAAAGA CTACAAAAAG 
GGAAAAGCCT GCACTAACAC CAAGTGTCTC ATCATTGGAG CTGGCCCCTG TGGTCTCCGT 
ACAGCCATTG ACTTATCCTT GTTGGGAGCT AAGGTAGTTG TTATTGAGAA ACGAGATGCC 
TTCTCCCGCA ACAACGTCTT GCATCTCTGG CCATTCACCA TACATGATCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTATGG CAAGTTCTGT GCTGGAGCTA TTGACCATAT CAGTATCCGT 
CAGCTTCAAC TAATACTTTT GAAAGTAGCC CTGATCCTAG GCATTGAGAT CCATGTCAAT 
GTGGAATTCC AAGGTCTTGT ACAGCCTCCC GAGGACCAAG AAAATGAACG GATAGGTTGG 
CGGGCACTGG TCCACCCCAA AACCCATCCT GTGTCAGAAT ATGAATTTGA AGTGATCATC 
GGAGGGGATG GCCGTAGGAA CACCTTGGAA GGATTTCGCC GGAAAGAATT TCGTGGCAAA 
CTGGCCATAG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTAAAGGAA 
GCCACAGGTA TTGACTTGGA GAATATCGTC TACTACAAAG ATGACACACA CTATTTTGTT 
ATGACAGCCA AAAAGCAGAG TTTGCTCGAC AAAGGGGTGA TACTGCATGA CTATGCCGAC 
ACAGAGCTCT TGCTTTCCCG GGAAAACGTG GACCAAGAGG CTCTGCTCAA CTATGCCAGG 
GAGGCGGCCG ACTTCTCCAC TCAGCAGCAG CTGCCATCTC TGGATTTTGC CATCAATCAC 
TATGGGCAGC CTGATGTGGC CATGTTTGAC TTCACCTGTA TGTACGCCTC TGAGAACGCT 
GCCCTGGTGC GGGAGCAGAA TGGACACCAG TTACTAGTGG CCCTGGTAGG GGACAGCCTC 
CTGGAGCCAT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GGTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTACGAAG TTGGTCTCTC GGAACAAGCC CCTTGGAAGT CTTGGCCGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACTACCCCTG AGAATGTGAG TAAAAACTTC 
AGCCAATACA GCATAGACCC TGTCACCAGA TATCCCAATA TTAACATCAA CTTCCTCCGC 
CCAAGCCAGG TGCGCCATTT ATACAATACT GGAGAAACAA GAGATATTCA TCTGGAAATG 
GAGAACCTGG TGAACTCCCG AACTACCCCA AAGTTGACTC GCAATGAGTC TGTAGCTCGT 
TCAAGCAAAC TGCTGGGTTG GTGCCAAAGG CAGACAGATG GCTATGCAGG GGTAAATGTG 
ACAGATCTTA CTATGTCTTG GAAAAGTGGA CTGGCCTTAT GTGCGATCAT CCATAGATAC 
CGCCCTGATT TGATAGATTT TGATTCTTTG GATGAGCAAA ATGTGGAGAA GAATAACCAG 
CTGGCCTTTG ATATTGCCGA GAAGGAACTG GGCATCTCTC CCATCATGAC AGGCAAAGAA 
ATGGCCTCGG TGGGGGAGCC TGACAAACTG TCTATGGTGA TGTACCTGAC TCAGTTCTAC 
GAGATGTTCA AGGACTCACT CCCCTCCAGC GATGCCCTGG ACCTGAATGC TGAGGAGAAA 
GCAGTTCTTA TAGCCAGCAC CAAATCACCC ATCTCCTTCC TCAGCAAACT TGGGCAGACC 
ATCTCTCGGA AGCGTTCTCC CAAGGATAAA AAGGAAAAGG ACTTGGATGG TGCTGGAAAG 
AGGAGAAAGA CCAGTCAGTC AGAAGAGGAG GAAGCACCCC GGGGCTACAG AGGAGGAAGG 
CCCACACTGG TGAGCACTCT GACAGACAGG AGGATGGATG TGGCCCTCGG CAATCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAATTTG AAGAGAATGC ACCCCCCCAG 
TCCACTGGCA TGAGGAGACA GGGCTCGATA AAGAAGGAGT TCCCACAGAA CTTAGGAGGC 
AGCGACACGT GCTATTTCTG CCGGAAGCGG GTCTACGTGA TGGAAAGGCT GAGTGCTGAG 
GGCAAGTTCT TCCATCGGAG CTGCTTCAAG TGCGAGTACT GCGCCACCAC CCTGCGCCTC 
TCGGCCTATG CCTATGACAT TGAGGACGGT AAATTCTACT GCAAACCACA CTACTGTTAT 
CGACTCTCTG GCTCTGCACA GAGGAAGAGA CCAGCAGTGG CTCCTCTCTC TGGAAAGGAG 
GCCAGAGAAC CCCTGCAGGA TGGCCCCACT GTGGACACAA ATGGACGGGC CAGCGCTGTC 
ACCAGCCCTG CTGAGAGAAC TCCAGGTTCA AGCGTGAATG GTGTGGAAGA GCCTAGCATT 
GCCAAGAGGC TCAGGGGCAC TCCCGAGCGG ATTGAGCTGG AGAACTACCG CCTGTCCGTG 
AAGCAGGCCG AGGAGCTGGA GGAGGTGCCC GAGGAGACAC AGGCCGAGCA CAACCTGAGC 
AGCGTGCTGG ACACGGGGGC AGAGGAGGAC GTGGCCAGCA GCAGTTCTGA GTCAGAGATG 
GAGGAAGAGG AGGAAGAAGA GCCTCCTCTG CCCACTTCGG ACCTGGGCGG TGTTCCTTGG 
AAGGAGGCCG TGAGGATCCA TGCTCTTCTG AAAGGAAAGA GTGAAGAGGA GCTAGAGGCC 
TCAAAGAGCT TTGGGCCTGG GCATGAAGAA GACGAGGAGG AGGAGGAGTA TGAAGAGGAG 
GAGGAGGAGG AGGAGGAAGA ATATGAAGAG GAAGAGGAAG AGGAAGAGTC TAGTGAAGTT 
GGGAGCCAGA GGCTACAGCA GATCGTAAAT CCGGCCGACC CCTTGGAGAT CCAGGCTGAT 
GTGCACTGGA CACATATCCG AGAGAGAGAG GAGGAGGAGC GAATGGTCCC AACCTCTGAG 
TCCTCCACTT CTAGAGTCCC ATTTGATGAG AATGACCTGG AGGAAGACGT GGACTCGGAG 
CCAGCAAAGA TAGAAGGGGA GGCCACAGAG AATGGGGACA CAGGGGACAC TGGTGCTGAG 
CTGGACGATG ATCAGCGCTG GTCTGACGGG ATCCCATCGG ATGCCGAGAC GGATCTCCGC 
GTGCAGCACC AGGAGGCGGT AGAGGCAGAC CTGGAGCTGA GGGTCTCAGA GAATGAGGAG 
GAGCCACCTG AGGTCACACC AGGGCATCCG GAGAGAAGTC TCACCCGGGT CCCCAGCCCT 
GCCCTGTCCC CTGAGGAGCA AGCTGGTCTG CGGTCCCCAG GCCACAGCCC TAAGGCAGAG 
GGGGCCCAAA TCCCACTTGT CTCTGTTGTC ACCAAGGCAA AATTGCCTGA AGAGAACCTT 
TTCCCCAAGC CTTTGCTCCC CAAAGTGAAG CCCAAGGTGG AAGTGCCCTG CAGTCAGAGA 
GCTGCATGCT CCCCCATCCG TTCTCAGCCA GTGGCTCTGC CAGAAGCCAG GACACCTACC 
TCCCCAGTGA GCTCGCAGTG CCTGTCGCCT TTGGCCACCT CCACCCCCAC CAGCACCCAG 
CTCCCCATTT GCTCCCAGCC TCAGCCTTCC TCTGAGACCA CGGCCCCATC CCCCACCAAG 
TCCCCCATAC GGCTCCAGCC TGTTTCAGCC AAAACATCTA TCCCAGTGGT TCCCCTCCCC 
TTGAAGAACC AAGGGGACAC CAAGGACAGG CTGGGCAGCC CTCTCGCTGT GGATGAGGCC 
CTGAAGCGGA ATGACCTGGT GGCAGAGTTC TGGATGAAGA GCGCCGAGAT CCGCCGCAGC 
CTCGGGCTCA CACCAGTGGA TCGGAGCAAG GTGTATGAGC CCAGCTTCCC TTCGCCTGCC 
TTTAAACCAG TGTCCCTAAA ATCCTATGCA GTGGAGAAGT CTCCCGAGGG GGAGGGACTC 
CAGCTTCTGA AGCCTCCACC TGTTCCTAAG AGACTAGGCC TGCCGAAGTC AGACGGTGAC 
CAGCCCTTCC TGCCAACCCC CAAGTCCCCG TCGGACAGAG AGCTGAAAAG CTCCCAGGAG 
GAGAGGAGAG ATCTGTCGAG CAGCTCCGGG CTGGGCCTTC ACGGGAGCTC CTCCAACATG 
AAGACTTTGG GCAGCCAGAG CTTCAACACA TCAGACTCCA CCATGCTCAC TCCCCCTTCA 
AGCCCACCGC CACCACCACC CCAAGACGAG GAACCCGCAA CTCTTCGGAG GAAGCCATAT 
CAGACTCATG AGCATAAGGA CGCTGAGCCC AAGGCCTCGG TGATTCCACC ACCACCACCT 
GCGACGTTCA TGCGGGCCCC CCGGGAGCCC ACCCAGCCCC TCCAAGAGGA GATGCGGAAG 
TCGTTTGTGG AGAGCGTGGA TGAGATCCCA TTTGCCGATG ATGTGGAGGA CACCTATGAT 
GACAAGACCG AGGACTCGAG TCTCCAGGAG AAGTTCTTCA CACCCCCCTC CTACTGGCCC 
CGCACGGAGA GGCCCCTCCA CCCACCCCTA GCCAAGGAGA ATGGTAGGTT GCCTACTCTG 
GAGAGTGGGG TCCAGCCACA GAAGAGGGGC CTGCCCTTAG TCTCTCCAGA AGCCAAGGAG 
CTGGCTGAGG AGCGCATGCG AGCCAGGGAG AAGTCTGTGA AGAGCCAGGC GTTGAGAGAC 
GCTATGGCCA AGCAGCTGAG TAAGATGAAG GAAATGGAGA TGGCGGCTGG GACCTCCAGT 
CCCCCAGGAG GCACCTCCCA CAAAGCCTCC TCTGTTCCCT CCAAGGGCAA AGACCTCAGC 
CCCGAGTCCC CTAGACGCCC AGTTCTCAAA GGCCCCAGGG AGCCTGCCCT GAAGCACGAA 
GCCACTAGTG AGGAGGTCCT CTCCCCACCA TCGGACTCAG GGGGCCCAGA TGGTTCGGTC 
ACCTCATCCG AGGGCTCCAG TGGGAAGAGC AAGAAGAGAT CGTCACTCTT CTCCCCCCGC 
AGAAACAAGA AAGAGAAGAA GTCCAAAGGT GAGGGCCGGC CCCCGGAGAA GCCCAGCCCA 
AGCCTTCTGG AGGAAGCTGC TGCCAAGCCC AAGTCCCTGT GGAAGTCGGT CTTTTCCGGG 
TACAAGAAGG ACAAAAAGAA GAAGGGTGAT GACAAGTCCT GTTCCAGCAC CCCTTCCAGC 
GGTGCCACTG TGGACTCTGG CAAGCACAAG ATGTCCCCTG TTGTGAGAGC AGAGCTGCAC 
CTGCGGCGCC AGTTGAGCTT CTCCGAGGAC TCAGACCTGT CCAGTGATGA CATCCTTGAG 
AGGTCCTCCC AGAAGTCCAG GCGAGAGTCG GTTTATGTAC CACACGCCTT GGCATTCAAG 
AGATCATATC CATCAAAGCC GAGAACCTAC ACAGAGGAGG AACTGAATGC CAAGCTGACC 
CGGCGTGTGC AGAAGGCAGC TCGTAGACAG GCCAAGCAAG AGGAGCTGAA GCGGCTGCAC 
CGAGCACAGA TCATCCAGAG GCAGCTGGAG CAGGTGGAGG AGAAGCAGAG ACAGCTGGAG 
GAAAGAGGGG TGGCCGTGGA GAAGGCGCTC CGTGGTGAAG CAGACTATTG GGGAGAGTCT 
TATTACAGTG ACCTCATCGA CTTGCATCTG GGTGTTGAGC CCAGTGGCGG GACTCCACGG 
CGTAGACCTC TCTCCTTCTG CCCTTGCTGT GTCCAGGAAG GCATGGGCAA GAAGGATGAC 
CCCAAGCTGA TGCAGGAGTG GTTCAAGTTG GTGCAAGAGA AGAATGCCAT GGTGCGCTAT 
GAGTCGGAGC TGATGATTTT TGCCCGGGAG CTGGAACTGG AGGACCGGCA GAGCCGACTG 
CAGCAGGAGC TCCGGGAGCG GATGGCGGTG GAAGATCACC TGAAGACTGA GGAGGAGCTG 
TCTGAGGAGA AGAAGATCTT GAACGAGATG CTGGAGGTGG TGGAGCAGAG AGATGCCCTC 
GTGGCCCTGC TTGAGGAGCA GCGACTCCGG GAGAAGGAGG AGGACAAAGA CCTGGAGGCT 
GTCATGCTGT CCAAGGGCTT CAGCCTGAAC TGGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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オンライン注文

MICAL3 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information: