APAF1 cDNA ORF clone, Canis lupus familiaris(dog)

The following APAF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the APAF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe17607 XM_014119302.2
Latest version!
Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe40119 XM_014119300.1
Latest version!
Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe293652 XM_022403383.1
Latest version!
Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe293652 XM_022403382.1
Latest version!
Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe293653 XM_022403384.1
Latest version!
Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe293654 XM_022403385.1
Latest version!
Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe293655 XM_022403386.1
Latest version!
Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe293656 XM_022403387.1
Latest version!
Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe293657 XM_022403388.1
Latest version!
Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe293658 XM_022403389.1
Latest version!
Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe293659 XM_022403390.1
Latest version!
Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe40116 XM_014119287.1
Latest version!
Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe40116 XM_014119288.1
Latest version!
Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe40116 XM_014119285.1
Latest version!
Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe40116 XM_014119286.1
Latest version!
Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe40116 XM_014119290.1
Latest version!
Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe40116 XM_014119291.1
Latest version!
Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe40116 XM_014119292.1
Latest version!
Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe40116 XM_014119289.1
Latest version!
Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe40116 XM_014119294.1
Latest version!
Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe40116 XM_014119293.1
Latest version!
Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe40116 XM_014119295.1
Latest version!
Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe40117 XM_014119296.1
Latest version!
Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe40118 XM_014119299.2
Latest version!
Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe40120 XM_014119301.1
Latest version!
Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe17604 XM_014119284.1
Latest version!
Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe17604 XM_005629260.2
Latest version!
Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe17604 XM_003432034.4
Latest version!
Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe17605 XM_014119297.1
Latest version!
Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe17606 XM_014119298.1
Latest version!
Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
OCe40118 XM_014119299.1 Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe17607 XM_014119302.1 Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe17604 XM_003432034.3 Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe17607
    Clone ID Related Accession (Same CDS sequence) XM_014119302.1 , XM_014119302.2
    Accession Version XM_014119302.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2472bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product apoptotic protease-activating factor 1 isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGGGA CTTCCTTATG TCTCTGGATG CTTTGCTATG
    TTTTTAACAT GCCATAGACT GGGCCTTGTG TGGTGTCCAG GCTGTGCATA CAGTCGATGG
    AGGCAAACTT CATGCCTGGC ACAGCAGAGG GCAGCAGTGA CAAGTTCTTT GGGATGTAAA
    ATCAGCAAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974777.1
    CDS175..2646
    Translation

    Target ORF information:

    RefSeq Version XM_014119302.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014119302.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGGGA CTTCCTTATG TCTCTGGATG CTTTGCTATG
    TTTTTAACAT GCCATAGACT GGGCCTTGTG TGGTGTCCAG GCTGTGCATA CAGTCGATGG
    AGGCAAACTT CATGCCTGGC ACAGCAGAGG GCAGCAGTGA CAAGTTCTTT GGGATGTAAA
    ATCAGCAAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe17607
    Clone ID Related Accession (Same CDS sequence) XM_014119302.1 , XM_014119302.2
    Accession Version XM_014119302.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2472bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product apoptotic protease-activating factor 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014119302.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGGGA CTTCCTTATG TCTCTGGATG CTTTGCTATG
    TTTTTAACAT GCCATAGACT GGGCCTTGTG TGGTGTCCAG GCTGTGCATA CAGTCGATGG
    AGGCAAACTT CATGCCTGGC ACAGCAGAGG GCAGCAGTGA CAAGTTCTTT GGGATGTAAA
    ATCAGCAAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974777.1
    CDS137..2608
    Translation

    Target ORF information:

    RefSeq Version XM_014119302.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014119302.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGGGA CTTCCTTATG TCTCTGGATG CTTTGCTATG
    TTTTTAACAT GCCATAGACT GGGCCTTGTG TGGTGTCCAG GCTGTGCATA CAGTCGATGG
    AGGCAAACTT CATGCCTGGC ACAGCAGAGG GCAGCAGTGA CAAGTTCTTT GGGATGTAAA
    ATCAGCAAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40119
    Clone ID Related Accession (Same CDS sequence) XM_014119300.1
    Accession Version XM_014119300.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2892bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product apoptotic protease-activating factor 1 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGGAGAAC TTGAGACAGA CCGAGGGTCG GATGGAGTTT
    AACAAGCTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974775.1
    CDS173..3064
    Translation

    Target ORF information:

    RefSeq Version XM_014119300.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014119300.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGGAGAAC TTGAGACAGA CCGAGGGTCG GATGGAGTTT
    AACAAGCTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe293652
    Clone ID Related Accession (Same CDS sequence) XM_022403383.1 , XM_022403382.1
    Accession Version XM_022403383.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product apoptotic protease-activating factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGATA TGGAGTATTA TTACTGGAAG AATAGAAAAA
    GACTTTGTCT GTCACCAGGA TACAGTACTT TCTTGTGATA TTTCTCCTGA TGCTACCAAG
    TTTTCATCTA CATCTGCAGA TAAGACTGCG AAGATTTGGA GTTTTGAACT TCTTTCACCT
    CTTCATGAAT TGAGGGGCCA CAAGGGCTGT GTGCGCTGCT CTGTCTTCTC TGTGGACAGT
    ACCCTGTTGG CGACTGGAGA TGACAATGGA GAAATCAGGA TATGGAATGT CTCAAATGGT
    GAGCTGCTTC ATTTGTGTGC TCCGATTTTG GTAGAAGAAG GAGCTGCTAC CCATGGAGGC
    TGGGTGACTG ACCTTTGCTT TTCTCCGGAT AGCAAAATGC TTGTCTCTGC TGGAGGATAT
    CTTAAGGCGA TCACCAGCCC AGGGACACCG TTCCACCCGT CTGTTTCCCT ACATGCTGCC
    AACACCTATG TCACTAAGTC TTGGAATGGC TGTTCAAGAA GCCACAAAAA ACGGGAACAC
    CATGCAATGC TAAAGAAGGC TGGGCAGATT CAGCAGGAAG AGGAACTGCC AGCCCGTGAG
    CTGGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022259091.1
    CDS168..3956
    Translation

    Target ORF information:

    RefSeq Version XM_022403383.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022403383.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGATA TGGAGTATTA TTACTGGAAG AATAGAAAAA
    GACTTTGTCT GTCACCAGGA TACAGTACTT TCTTGTGATA TTTCTCCTGA TGCTACCAAG
    TTTTCATCTA CATCTGCAGA TAAGACTGCG AAGATTTGGA GTTTTGAACT TCTTTCACCT
    CTTCATGAAT TGAGGGGCCA CAAGGGCTGT GTGCGCTGCT CTGTCTTCTC TGTGGACAGT
    ACCCTGTTGG CGACTGGAGA TGACAATGGA GAAATCAGGA TATGGAATGT CTCAAATGGT
    GAGCTGCTTC ATTTGTGTGC TCCGATTTTG GTAGAAGAAG GAGCTGCTAC CCATGGAGGC
    TGGGTGACTG ACCTTTGCTT TTCTCCGGAT AGCAAAATGC TTGTCTCTGC TGGAGGATAT
    CTTAAGGCGA TCACCAGCCC AGGGACACCG TTCCACCCGT CTGTTTCCCT ACATGCTGCC
    AACACCTATG TCACTAAGTC TTGGAATGGC TGTTCAAGAA GCCACAAAAA ACGGGAACAC
    CATGCAATGC TAAAGAAGGC TGGGCAGATT CAGCAGGAAG AGGAACTGCC AGCCCGTGAG
    CTGGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe293652
    Clone ID Related Accession (Same CDS sequence) XM_022403383.1 , XM_022403382.1
    Accession Version XM_022403382.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product apoptotic protease-activating factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGATA TGGAGTATTA TTACTGGAAG AATAGAAAAA
    GACTTTGTCT GTCACCAGGA TACAGTACTT TCTTGTGATA TTTCTCCTGA TGCTACCAAG
    TTTTCATCTA CATCTGCAGA TAAGACTGCG AAGATTTGGA GTTTTGAACT TCTTTCACCT
    CTTCATGAAT TGAGGGGCCA CAAGGGCTGT GTGCGCTGCT CTGTCTTCTC TGTGGACAGT
    ACCCTGTTGG CGACTGGAGA TGACAATGGA GAAATCAGGA TATGGAATGT CTCAAATGGT
    GAGCTGCTTC ATTTGTGTGC TCCGATTTTG GTAGAAGAAG GAGCTGCTAC CCATGGAGGC
    TGGGTGACTG ACCTTTGCTT TTCTCCGGAT AGCAAAATGC TTGTCTCTGC TGGAGGATAT
    CTTAAGGCGA TCACCAGCCC AGGGACACCG TTCCACCCGT CTGTTTCCCT ACATGCTGCC
    AACACCTATG TCACTAAGTC TTGGAATGGC TGTTCAAGAA GCCACAAAAA ACGGGAACAC
    CATGCAATGC TAAAGAAGGC TGGGCAGATT CAGCAGGAAG AGGAACTGCC AGCCCGTGAG
    CTGGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022259090.1
    CDS137..3925
    Translation

    Target ORF information:

    RefSeq Version XM_022403382.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022403382.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGATA TGGAGTATTA TTACTGGAAG AATAGAAAAA
    GACTTTGTCT GTCACCAGGA TACAGTACTT TCTTGTGATA TTTCTCCTGA TGCTACCAAG
    TTTTCATCTA CATCTGCAGA TAAGACTGCG AAGATTTGGA GTTTTGAACT TCTTTCACCT
    CTTCATGAAT TGAGGGGCCA CAAGGGCTGT GTGCGCTGCT CTGTCTTCTC TGTGGACAGT
    ACCCTGTTGG CGACTGGAGA TGACAATGGA GAAATCAGGA TATGGAATGT CTCAAATGGT
    GAGCTGCTTC ATTTGTGTGC TCCGATTTTG GTAGAAGAAG GAGCTGCTAC CCATGGAGGC
    TGGGTGACTG ACCTTTGCTT TTCTCCGGAT AGCAAAATGC TTGTCTCTGC TGGAGGATAT
    CTTAAGGCGA TCACCAGCCC AGGGACACCG TTCCACCCGT CTGTTTCCCT ACATGCTGCC
    AACACCTATG TCACTAAGTC TTGGAATGGC TGTTCAAGAA GCCACAAAAA ACGGGAACAC
    CATGCAATGC TAAAGAAGGC TGGGCAGATT CAGCAGGAAG AGGAACTGCC AGCCCGTGAG
    CTGGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe293653
    Clone ID Related Accession (Same CDS sequence) XM_022403384.1
    Accession Version XM_022403384.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3738bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product apoptotic protease-activating factor 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGGAT ACAGTACTTT CTTGTGATAT TTCTCCTGAT
    GCTACCAAGT TTTCATCTAC ATCTGCAGAT AAGACTGCGA AGATTTGGAG TTTTGAACTT
    CTTTCACCTC TTCATGAATT GAGGGGCCAC AAGGGCTGTG TGCGCTGCTC TGTCTTCTCT
    GTGGACAGTA CCCTGTTGGC GACTGGAGAT GACAATGGAG AAATCAGGAT ATGGAATGTC
    TCAAATGGTG AGCTGCTTCA TTTGTGTGCT CCGATTTTGG TAGAAGAAGG AGCTGCTACC
    CATGGAGGCT GGGTGACTGA CCTTTGCTTT TCTCCGGATA GCAAAATGCT TGTCTCTGCT
    GGAGGATATC TTAAGGCGAT CACCAGCCCA GGGACACCGT TCCACCCGTC TGTTTCCCTA
    CATGCTGCCA ACACCTATGT CACTAAGTCT TGGAATGGCT GTTCAAGAAG CCACAAAAAA
    CGGGAACACC ATGCAATGCT AAAGAAGGCT GGGCAGATTC AGCAGGAAGA GGAACTGCCA
    GCCCGTGAGC TGGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022259092.1
    CDS137..3874
    Translation

    Target ORF information:

    RefSeq Version XM_022403384.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022403384.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGGAT ACAGTACTTT CTTGTGATAT TTCTCCTGAT
    GCTACCAAGT TTTCATCTAC ATCTGCAGAT AAGACTGCGA AGATTTGGAG TTTTGAACTT
    CTTTCACCTC TTCATGAATT GAGGGGCCAC AAGGGCTGTG TGCGCTGCTC TGTCTTCTCT
    GTGGACAGTA CCCTGTTGGC GACTGGAGAT GACAATGGAG AAATCAGGAT ATGGAATGTC
    TCAAATGGTG AGCTGCTTCA TTTGTGTGCT CCGATTTTGG TAGAAGAAGG AGCTGCTACC
    CATGGAGGCT GGGTGACTGA CCTTTGCTTT TCTCCGGATA GCAAAATGCT TGTCTCTGCT
    GGAGGATATC TTAAGGCGAT CACCAGCCCA GGGACACCGT TCCACCCGTC TGTTTCCCTA
    CATGCTGCCA ACACCTATGT CACTAAGTCT TGGAATGGCT GTTCAAGAAG CCACAAAAAA
    CGGGAACACC ATGCAATGCT AAAGAAGGCT GGGCAGATTC AGCAGGAAGA GGAACTGCCA
    GCCCGTGAGC TGGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe293654
    Clone ID Related Accession (Same CDS sequence) XM_022403385.1
    Accession Version XM_022403385.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3663bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product apoptotic protease-activating factor 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTTTGGGAT GTAAAATCAG CAAATGAGAG GAAAAGCATT
    AATGTGAAGC AGTTCTTCCT AAATTCAGAG GAGCCTCAAG AGGATATGGA AGTGATAGTG
    AAATGTTGTT CATGGTCTGC TGATGGCACT AGGATAATGG TGGCAGCAAA AAATAAAATC
    TTTCTATTTG ACATTCATAC TGGTGGCTTA TTGGCAGAAA TCCATACAGG CCATCACAGC
    ACCATCCAGT ACTGTGACTT CTCCCCCCAA AATCATTTGG CAGTAGTGGC TTTGTCCCAG
    TGCTGTGTGG AGTTGTGGAA CATGGACTCC TGTTTAAAAG TGGCTGATTG CAGAGGACAT
    TTAAGTTGGG TTCATTGTGT GATGTTTTCT CCCGATGGAT CATCATTTTT GACATCTTCT
    GATGACCAGA CAATCAGGCT CTGGGAGACC AAAAAAGTAT GTAAGAACTC TGCTATAGTG
    TTAAAGCAAG AAATAGATGT TGTGTTTCAA GAAAATGAAG TGATGGTTCT TGCAGTTGAC
    AATATAAAAC GTCTGCAGCT CATTAATGGA AAAACAGGTC AGATTGATTA TCTGATTGAA
    GCTCAGGTTA GTTGCTGTTG CCTAAGTCCA CATCTTCAGT ACATTGCATT TGGAGGTGAA
    GATGGAGCCG TTGAGATTTT AGAACTCCTA AACAACAGAA TCTTCCAATC CAGGATTGGG
    CACAAGAAAA CTGTGCGGCA CATCCAGTTC ACAGATGATG GGAAGACTCT TATTTCAAGC
    TCTGATGATT CTTCAATTCA GGTATGGAAT TGGCAGTCAG AGGAATATGT CTTCCTACAA
    GCCCATCAGG AAACTGTGAA AGACTTTAAG CTCCTGAAAA ATTCAAAACT GCTTTCCTGG
    TCATTTGATG GAACAGTAAA GATATGGAGT ATTATTACTG GAAGAATAGA AAAAGACTTT
    GTCTGTCACC AGGATACAGT ACTTTCTTGT GATATTTCTC CTGATGCTAC CAAGTTTTCA
    TCTACATCTG CAGATAAGAC TGCGAAGATT TGGAGTTTTG AACTTCTTTC ACCTCTTCAT
    GAATTGAGGG GCCACAAGGG CTGTGTGCGC TGCTCTGTCT TCTCTGTGGA CAGTACCCTG
    TTGGCGACTG GAGATGACAA TGGAGAAATC AGGATATGGA ATGTCTCAAA TGGTGAGCTG
    CTTCATTTGT GTGCTCCGAT TTTGGTAGAA GAAGGAGCTG CTACCCATGG AGGCTGGGTG
    ACTGACCTTT GCTTTTCTCC GGATAGCAAA ATGCTTGTCT CTGCTGGAGG ATATCTTAAG
    GCGATCACCA GCCCAGGGAC ACCGTTCCAC CCGTCTGTTT CCCTACATGC TGCCAACACC
    TATGTCACTA AGTCTTGGAA TGGCTGTTCA AGAAGCCACA AAAAACGGGA ACACCATGCA
    ATGCTAAAGA AGGCTGGGCA GATTCAGCAG GAAGAGGAAC TGCCAGCCCG TGAGCTGGCA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022259093.1
    CDS137..3799
    Translation

    Target ORF information:

    RefSeq Version XM_022403385.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022403385.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTTTGGGAT GTAAAATCAG CAAATGAGAG GAAAAGCATT
    AATGTGAAGC AGTTCTTCCT AAATTCAGAG GAGCCTCAAG AGGATATGGA AGTGATAGTG
    AAATGTTGTT CATGGTCTGC TGATGGCACT AGGATAATGG TGGCAGCAAA AAATAAAATC
    TTTCTATTTG ACATTCATAC TGGTGGCTTA TTGGCAGAAA TCCATACAGG CCATCACAGC
    ACCATCCAGT ACTGTGACTT CTCCCCCCAA AATCATTTGG CAGTAGTGGC TTTGTCCCAG
    TGCTGTGTGG AGTTGTGGAA CATGGACTCC TGTTTAAAAG TGGCTGATTG CAGAGGACAT
    TTAAGTTGGG TTCATTGTGT GATGTTTTCT CCCGATGGAT CATCATTTTT GACATCTTCT
    GATGACCAGA CAATCAGGCT CTGGGAGACC AAAAAAGTAT GTAAGAACTC TGCTATAGTG
    TTAAAGCAAG AAATAGATGT TGTGTTTCAA GAAAATGAAG TGATGGTTCT TGCAGTTGAC
    AATATAAAAC GTCTGCAGCT CATTAATGGA AAAACAGGTC AGATTGATTA TCTGATTGAA
    GCTCAGGTTA GTTGCTGTTG CCTAAGTCCA CATCTTCAGT ACATTGCATT TGGAGGTGAA
    GATGGAGCCG TTGAGATTTT AGAACTCCTA AACAACAGAA TCTTCCAATC CAGGATTGGG
    CACAAGAAAA CTGTGCGGCA CATCCAGTTC ACAGATGATG GGAAGACTCT TATTTCAAGC
    TCTGATGATT CTTCAATTCA GGTATGGAAT TGGCAGTCAG AGGAATATGT CTTCCTACAA
    GCCCATCAGG AAACTGTGAA AGACTTTAAG CTCCTGAAAA ATTCAAAACT GCTTTCCTGG
    TCATTTGATG GAACAGTAAA GATATGGAGT ATTATTACTG GAAGAATAGA AAAAGACTTT
    GTCTGTCACC AGGATACAGT ACTTTCTTGT GATATTTCTC CTGATGCTAC CAAGTTTTCA
    TCTACATCTG CAGATAAGAC TGCGAAGATT TGGAGTTTTG AACTTCTTTC ACCTCTTCAT
    GAATTGAGGG GCCACAAGGG CTGTGTGCGC TGCTCTGTCT TCTCTGTGGA CAGTACCCTG
    TTGGCGACTG GAGATGACAA TGGAGAAATC AGGATATGGA ATGTCTCAAA TGGTGAGCTG
    CTTCATTTGT GTGCTCCGAT TTTGGTAGAA GAAGGAGCTG CTACCCATGG AGGCTGGGTG
    ACTGACCTTT GCTTTTCTCC GGATAGCAAA ATGCTTGTCT CTGCTGGAGG ATATCTTAAG
    GCGATCACCA GCCCAGGGAC ACCGTTCCAC CCGTCTGTTT CCCTACATGC TGCCAACACC
    TATGTCACTA AGTCTTGGAA TGGCTGTTCA AGAAGCCACA AAAAACGGGA ACACCATGCA
    ATGCTAAAGA AGGCTGGGCA GATTCAGCAG GAAGAGGAAC TGCCAGCCCG TGAGCTGGCA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe293655
    Clone ID Related Accession (Same CDS sequence) XM_022403386.1
    Accession Version XM_022403386.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3627bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product apoptotic protease-activating factor 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTA TTTGACATTC ATACTGGTGG CTTATTGGCA
    GAAATCCATA CAGGCCATCA CAGCACCATC CAGTACTGTG ACTTCTCCCC CCAAAATCAT
    TTGGCAGTAG TGGCTTTGTC CCAGTGCTGT GTGGAGTTGT GGAACATGGA CTCCTGTTTA
    AAAGTGGCTG ATTGCAGAGG ACATTTAAGT TGGGTTCATT GTGTGATGTT TTCTCCCGAT
    GGATCATCAT TTTTGACATC TTCTGATGAC CAGACAATCA GGCTCTGGGA GACCAAAAAA
    GTATGTAAGA ACTCTGCTAT AGTGTTAAAG CAAGAAATAG ATGTTGTGTT TCAAGAAAAT
    GAAGTGATGG TTCTTGCAGT TGACAATATA AAACGTCTGC AGCTCATTAA TGGAAAAACA
    GGTCAGATTG ATTATCTGAT TGAAGCTCAG GTTAGTTGCT GTTGCCTAAG TCCACATCTT
    CAGTACATTG CATTTGGAGG TGAAGATGGA GCCGTTGAGA TTTTAGAACT CCTAAACAAC
    AGAATCTTCC AATCCAGGAT TGGGCACAAG AAAACTGTGC GGCACATCCA GTTCACAGAT
    GATGGGAAGA CTCTTATTTC AAGCTCTGAT GATTCTTCAA TTCAGGTATG GAATTGGCAG
    TCAGAGGAAT ATGTCTTCCT ACAAGCCCAT CAGGAAACTG TGAAAGACTT TAAGCTCCTG
    AAAAATTCAA AACTGCTTTC CTGGTCATTT GATGGAACAG TAAAGATATG GAGTATTATT
    ACTGGAAGAA TAGAAAAAGA CTTTGTCTGT CACCAGGATA CAGTACTTTC TTGTGATATT
    TCTCCTGATG CTACCAAGTT TTCATCTACA TCTGCAGATA AGACTGCGAA GATTTGGAGT
    TTTGAACTTC TTTCACCTCT TCATGAATTG AGGGGCCACA AGGGCTGTGT GCGCTGCTCT
    GTCTTCTCTG TGGACAGTAC CCTGTTGGCG ACTGGAGATG ACAATGGAGA AATCAGGATA
    TGGAATGTCT CAAATGGTGA GCTGCTTCAT TTGTGTGCTC CGATTTTGGT AGAAGAAGGA
    GCTGCTACCC ATGGAGGCTG GGTGACTGAC CTTTGCTTTT CTCCGGATAG CAAAATGCTT
    GTCTCTGCTG GAGGATATCT TAAGGCGATC ACCAGCCCAG GGACACCGTT CCACCCGTCT
    GTTTCCCTAC ATGCTGCCAA CACCTATGTC ACTAAGTCTT GGAATGGCTG TTCAAGAAGC
    CACAAAAAAC GGGAACACCA TGCAATGCTA AAGAAGGCTG GGCAGATTCA GCAGGAAGAG
    GAACTGCCAG CCCGTGAGCT GGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022259094.1
    CDS137..3763
    Translation

    Target ORF information:

    RefSeq Version XM_022403386.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022403386.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTA TTTGACATTC ATACTGGTGG CTTATTGGCA
    GAAATCCATA CAGGCCATCA CAGCACCATC CAGTACTGTG ACTTCTCCCC CCAAAATCAT
    TTGGCAGTAG TGGCTTTGTC CCAGTGCTGT GTGGAGTTGT GGAACATGGA CTCCTGTTTA
    AAAGTGGCTG ATTGCAGAGG ACATTTAAGT TGGGTTCATT GTGTGATGTT TTCTCCCGAT
    GGATCATCAT TTTTGACATC TTCTGATGAC CAGACAATCA GGCTCTGGGA GACCAAAAAA
    GTATGTAAGA ACTCTGCTAT AGTGTTAAAG CAAGAAATAG ATGTTGTGTT TCAAGAAAAT
    GAAGTGATGG TTCTTGCAGT TGACAATATA AAACGTCTGC AGCTCATTAA TGGAAAAACA
    GGTCAGATTG ATTATCTGAT TGAAGCTCAG GTTAGTTGCT GTTGCCTAAG TCCACATCTT
    CAGTACATTG CATTTGGAGG TGAAGATGGA GCCGTTGAGA TTTTAGAACT CCTAAACAAC
    AGAATCTTCC AATCCAGGAT TGGGCACAAG AAAACTGTGC GGCACATCCA GTTCACAGAT
    GATGGGAAGA CTCTTATTTC AAGCTCTGAT GATTCTTCAA TTCAGGTATG GAATTGGCAG
    TCAGAGGAAT ATGTCTTCCT ACAAGCCCAT CAGGAAACTG TGAAAGACTT TAAGCTCCTG
    AAAAATTCAA AACTGCTTTC CTGGTCATTT GATGGAACAG TAAAGATATG GAGTATTATT
    ACTGGAAGAA TAGAAAAAGA CTTTGTCTGT CACCAGGATA CAGTACTTTC TTGTGATATT
    TCTCCTGATG CTACCAAGTT TTCATCTACA TCTGCAGATA AGACTGCGAA GATTTGGAGT
    TTTGAACTTC TTTCACCTCT TCATGAATTG AGGGGCCACA AGGGCTGTGT GCGCTGCTCT
    GTCTTCTCTG TGGACAGTAC CCTGTTGGCG ACTGGAGATG ACAATGGAGA AATCAGGATA
    TGGAATGTCT CAAATGGTGA GCTGCTTCAT TTGTGTGCTC CGATTTTGGT AGAAGAAGGA
    GCTGCTACCC ATGGAGGCTG GGTGACTGAC CTTTGCTTTT CTCCGGATAG CAAAATGCTT
    GTCTCTGCTG GAGGATATCT TAAGGCGATC ACCAGCCCAG GGACACCGTT CCACCCGTCT
    GTTTCCCTAC ATGCTGCCAA CACCTATGTC ACTAAGTCTT GGAATGGCTG TTCAAGAAGC
    CACAAAAAAC GGGAACACCA TGCAATGCTA AAGAAGGCTG GGCAGATTCA GCAGGAAGAG
    GAACTGCCAG CCCGTGAGCT GGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe293656
    Clone ID Related Accession (Same CDS sequence) XM_022403387.1
    Accession Version XM_022403387.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3621bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product apoptotic protease-activating factor 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGAT
    CTACATAAGA AGATAGTCAC TCAGTTCCAG AAACATTACC AGCCACATAC TCTTTCACCA
    GATCAGGAGG ACTGCATGTA CTGGTACAAT TTTCTGGCCT ACCACATGGC TAGTGCCAGT
    ATGCACAAAG AACTTTGTGC TTTAATGTTT TCCTTGGATT GGATTAAAGC AAAAACTGAA
    CTCGTAGGCC CTGCTCATCT GATTCATGAA TTTGTGGAAT ACAGGCATAT ATTGGATGAA
    AAGGATTGTG CAGTCTGTGA AAATTTCCAG GAATTTTTAT CTTTAAATGG ACATCTTCTT
    GGACGACAGC CATTTCCTAA TATTGTACAA CTGGGTCTCT GTGAACCAGA AACTTCAGAA
    GTTTATCAGC AAGCTAAGCT GCAGGCCAAG CAGGAGGTCG ATCACGGAAT GCTTTACCTG
    GAGTGGATAA ACAAAAAAAA CATCAAGAAT CTTTCCCGCT TAGTTGTCCG CCCCCATACA
    GATGCTGTTT ACCATGCTTG CTTTTCTGAG GATGGTCAAA GAATAGCTTC TTGTGGAGTT
    GATAAAACCT TACAGGTGTT CAAAGCTGAA ACAGGAGAGA AACTGCTAGA AATCAAGGCT
    CATGAAGATG AAGTGCTTTG TTGTGCATTC TCTACAGATG ACAGATTTAT AGCAACCTGC
    TCAGTGGATA AAAAAGTGAA GATTTGGAAT TCTATGACAG GGGGACTAGT ACATGTCTAT
    GATGAGCACT CAGAGCAAGT CAACTGCTGC CACTTTACCA ACAACAGTCA TTACCTTCTC
    TTAGCCACTG CATCAAGTGA CTGCTTCCTC AAACTCTGGG ATTTGAATCA AAAAGAATGT
    CGAAATACCA TGTTTGGCCA TACAAATTCA GTCAATCACT GCAGATTTTC ACCAGATGAT
    AAGCTTTTGG CTAGTTGTTC AGCTGATGGA ACATTAAAGC TTTGGGATGT AAAATCAGCA
    AATGAGAGGA AAAGCATTAA TGTGAAGCAG TTCTTCCTAA ATTCAGAGGA GCCTCAAGAG
    GATATGGAAG TGATAGTGAA ATGTTGTTCA TGGTCTGCTG ATGGCACTAG GATAATGGTG
    GCAGCAAAAA ATAAAATCTT TCTATTTGAC ATTCATACTG GTGGCTTATT GGCAGAAATC
    CATACAGGCC ATCACAGCAC CATCCAGTAC TGTGACTTCT CCCCCCAAAA TCATTTGGCA
    GTAGTGGCTT TGTCCCAGTG CTGTGTGGAG TTGTGGAACA TGGACTCCTG TTTAAAAGTG
    GCTGATTGCA GAGGACATTT AAGTTGGGTT CATTGTGTGA TGTTTTCTCC CGATGGATCA
    TCATTTTTGA CATCTTCTGA TGACCAGACA ATCAGGCTCT GGGAGACCAA AAAAGTATGT
    AAGAACTCTG CTATAGTGTT AAAGCAAGAA ATAGATGTTG TGTTTCAAGA AAATGAAGTG
    ATGGTTCTTG CAGTTGACAA TATAAAACGT CTGCAGCTCA TTAATGGAAA AACAGGTCAG
    ATTGATTATC TGATTGAAGC TCAGGTTAGT TGCTGTTGCC TAAGTCCACA TCTTCAGTAC
    ATTGCATTTG GAGGTGAAGA TGGAGCCGTT GAGATTTTAG AACTCCTAAA CAACAGAATC
    TTCCAATCCA GGATTGGGCA CAAGAAAACT GTGCGGCACA TCCAGTTCAC AGATGATGGG
    AAGACTCTTA TTTCAAGCTC TGATGATTCT TCAATTCAGG TATGGAATTG GCAGTCAGAG
    GAATATGTCT TCCTACAAGC CCATCAGGAA ACTGTGAAAG ACTTTAAGCT CCTGAAAAAT
    TCAAAACTGC TTTCCTGGTC ATTTGATGGA ACAGTAAAGA TATGGAGTAT TATTACTGGA
    AGAATAGAAA AAGACTTTGT CTGTCACCAG GATACAGTAC TTTCTTGTGA TATTTCTCCT
    GATGCTACCA AGTTTTCATC TACATCTGCA GATAAGACTG CGAAGATTTG GAGTTTTGAA
    CTTCTTTCAC CTCTTCATGA ATTGAGGGGC CACAAGGGCT GTGTGCGCTG CTCTGTCTTC
    TCTGTGGACA GTACCCTGTT GGCGACTGGA GATGACAATG GAGAAATCAG GATATGGAAT
    GTCTCAAATG GTGAGCTGCT TCATTTGTGT GCTCCGATTT TGGTAGAAGA AGGAGCTGCT
    ACCCATGGAG GCTGGGTGAC TGACCTTTGC TTTTCTCCGG ATAGCAAAAT GCTTGTCTCT
    GCTGGAGGAT ATCTTAAGGC GATCACCAGC CCAGGGACAC CGTTCCACCC GTCTGTTTCC
    CTACATGCTG CCAACACCTA TGTCACTAAG TCTTGGAATG GCTGTTCAAG AAGCCACAAA
    AAACGGGAAC ACCATGCAAT GCTAAAGAAG GCTGGGCAGA TTCAGCAGGA AGAGGAACTG
    CCAGCCCGTG AGCTGGCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022259095.1
    CDS137..3757
    Translation

    Target ORF information:

    RefSeq Version XM_022403387.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022403387.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGAT
    CTACATAAGA AGATAGTCAC TCAGTTCCAG AAACATTACC AGCCACATAC TCTTTCACCA
    GATCAGGAGG ACTGCATGTA CTGGTACAAT TTTCTGGCCT ACCACATGGC TAGTGCCAGT
    ATGCACAAAG AACTTTGTGC TTTAATGTTT TCCTTGGATT GGATTAAAGC AAAAACTGAA
    CTCGTAGGCC CTGCTCATCT GATTCATGAA TTTGTGGAAT ACAGGCATAT ATTGGATGAA
    AAGGATTGTG CAGTCTGTGA AAATTTCCAG GAATTTTTAT CTTTAAATGG ACATCTTCTT
    GGACGACAGC CATTTCCTAA TATTGTACAA CTGGGTCTCT GTGAACCAGA AACTTCAGAA
    GTTTATCAGC AAGCTAAGCT GCAGGCCAAG CAGGAGGTCG ATCACGGAAT GCTTTACCTG
    GAGTGGATAA ACAAAAAAAA CATCAAGAAT CTTTCCCGCT TAGTTGTCCG CCCCCATACA
    GATGCTGTTT ACCATGCTTG CTTTTCTGAG GATGGTCAAA GAATAGCTTC TTGTGGAGTT
    GATAAAACCT TACAGGTGTT CAAAGCTGAA ACAGGAGAGA AACTGCTAGA AATCAAGGCT
    CATGAAGATG AAGTGCTTTG TTGTGCATTC TCTACAGATG ACAGATTTAT AGCAACCTGC
    TCAGTGGATA AAAAAGTGAA GATTTGGAAT TCTATGACAG GGGGACTAGT ACATGTCTAT
    GATGAGCACT CAGAGCAAGT CAACTGCTGC CACTTTACCA ACAACAGTCA TTACCTTCTC
    TTAGCCACTG CATCAAGTGA CTGCTTCCTC AAACTCTGGG ATTTGAATCA AAAAGAATGT
    CGAAATACCA TGTTTGGCCA TACAAATTCA GTCAATCACT GCAGATTTTC ACCAGATGAT
    AAGCTTTTGG CTAGTTGTTC AGCTGATGGA ACATTAAAGC TTTGGGATGT AAAATCAGCA
    AATGAGAGGA AAAGCATTAA TGTGAAGCAG TTCTTCCTAA ATTCAGAGGA GCCTCAAGAG
    GATATGGAAG TGATAGTGAA ATGTTGTTCA TGGTCTGCTG ATGGCACTAG GATAATGGTG
    GCAGCAAAAA ATAAAATCTT TCTATTTGAC ATTCATACTG GTGGCTTATT GGCAGAAATC
    CATACAGGCC ATCACAGCAC CATCCAGTAC TGTGACTTCT CCCCCCAAAA TCATTTGGCA
    GTAGTGGCTT TGTCCCAGTG CTGTGTGGAG TTGTGGAACA TGGACTCCTG TTTAAAAGTG
    GCTGATTGCA GAGGACATTT AAGTTGGGTT CATTGTGTGA TGTTTTCTCC CGATGGATCA
    TCATTTTTGA CATCTTCTGA TGACCAGACA ATCAGGCTCT GGGAGACCAA AAAAGTATGT
    AAGAACTCTG CTATAGTGTT AAAGCAAGAA ATAGATGTTG TGTTTCAAGA AAATGAAGTG
    ATGGTTCTTG CAGTTGACAA TATAAAACGT CTGCAGCTCA TTAATGGAAA AACAGGTCAG
    ATTGATTATC TGATTGAAGC TCAGGTTAGT TGCTGTTGCC TAAGTCCACA TCTTCAGTAC
    ATTGCATTTG GAGGTGAAGA TGGAGCCGTT GAGATTTTAG AACTCCTAAA CAACAGAATC
    TTCCAATCCA GGATTGGGCA CAAGAAAACT GTGCGGCACA TCCAGTTCAC AGATGATGGG
    AAGACTCTTA TTTCAAGCTC TGATGATTCT TCAATTCAGG TATGGAATTG GCAGTCAGAG
    GAATATGTCT TCCTACAAGC CCATCAGGAA ACTGTGAAAG ACTTTAAGCT CCTGAAAAAT
    TCAAAACTGC TTTCCTGGTC ATTTGATGGA ACAGTAAAGA TATGGAGTAT TATTACTGGA
    AGAATAGAAA AAGACTTTGT CTGTCACCAG GATACAGTAC TTTCTTGTGA TATTTCTCCT
    GATGCTACCA AGTTTTCATC TACATCTGCA GATAAGACTG CGAAGATTTG GAGTTTTGAA
    CTTCTTTCAC CTCTTCATGA ATTGAGGGGC CACAAGGGCT GTGTGCGCTG CTCTGTCTTC
    TCTGTGGACA GTACCCTGTT GGCGACTGGA GATGACAATG GAGAAATCAG GATATGGAAT
    GTCTCAAATG GTGAGCTGCT TCATTTGTGT GCTCCGATTT TGGTAGAAGA AGGAGCTGCT
    ACCCATGGAG GCTGGGTGAC TGACCTTTGC TTTTCTCCGG ATAGCAAAAT GCTTGTCTCT
    GCTGGAGGAT ATCTTAAGGC GATCACCAGC CCAGGGACAC CGTTCCACCC GTCTGTTTCC
    CTACATGCTG CCAACACCTA TGTCACTAAG TCTTGGAATG GCTGTTCAAG AAGCCACAAA
    AAACGGGAAC ACCATGCAAT GCTAAAGAAG GCTGGGCAGA TTCAGCAGGA AGAGGAACTG
    CCAGCCCGTG AGCTGGCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe293657
    Clone ID Related Accession (Same CDS sequence) XM_022403388.1
    Accession Version XM_022403388.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3498bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product apoptotic protease-activating factor 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGTTG TGGAACATGG ACTCCTGTTT AAAAGTGGCT
    GATTGCAGAG GACATTTAAG TTGGGTTCAT TGTGTGATGT TTTCTCCCGA TGGATCATCA
    TTTTTGACAT CTTCTGATGA CCAGACAATC AGGCTCTGGG AGACCAAAAA AGTATGTAAG
    AACTCTGCTA TAGTGTTAAA GCAAGAAATA GATGTTGTGT TTCAAGAAAA TGAAGTGATG
    GTTCTTGCAG TTGACAATAT AAAACGTCTG CAGCTCATTA ATGGAAAAAC AGGTCAGATT
    GATTATCTGA TTGAAGCTCA GGTTAGTTGC TGTTGCCTAA GTCCACATCT TCAGTACATT
    GCATTTGGAG GTGAAGATGG AGCCGTTGAG ATTTTAGAAC TCCTAAACAA CAGAATCTTC
    CAATCCAGGA TTGGGCACAA GAAAACTGTG CGGCACATCC AGTTCACAGA TGATGGGAAG
    ACTCTTATTT CAAGCTCTGA TGATTCTTCA ATTCAGGTAT GGAATTGGCA GTCAGAGGAA
    TATGTCTTCC TACAAGCCCA TCAGGAAACT GTGAAAGACT TTAAGCTCCT GAAAAATTCA
    AAACTGCTTT CCTGGTCATT TGATGGAACA GTAAAGATAT GGAGTATTAT TACTGGAAGA
    ATAGAAAAAG ACTTTGTCTG TCACCAGGAT ACAGTACTTT CTTGTGATAT TTCTCCTGAT
    GCTACCAAGT TTTCATCTAC ATCTGCAGAT AAGACTGCGA AGATTTGGAG TTTTGAACTT
    CTTTCACCTC TTCATGAATT GAGGGGCCAC AAGGGCTGTG TGCGCTGCTC TGTCTTCTCT
    GTGGACAGTA CCCTGTTGGC GACTGGAGAT GACAATGGAG AAATCAGGAT ATGGAATGTC
    TCAAATGGTG AGCTGCTTCA TTTGTGTGCT CCGATTTTGG TAGAAGAAGG AGCTGCTACC
    CATGGAGGCT GGGTGACTGA CCTTTGCTTT TCTCCGGATA GCAAAATGCT TGTCTCTGCT
    GGAGGATATC TTAAGGCGAT CACCAGCCCA GGGACACCGT TCCACCCGTC TGTTTCCCTA
    CATGCTGCCA ACACCTATGT CACTAAGTCT TGGAATGGCT GTTCAAGAAG CCACAAAAAA
    CGGGAACACC ATGCAATGCT AAAGAAGGCT GGGCAGATTC AGCAGGAAGA GGAACTGCCA
    GCCCGTGAGC TGGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022259096.1
    CDS137..3634
    Translation

    Target ORF information:

    RefSeq Version XM_022403388.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022403388.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGTTG TGGAACATGG ACTCCTGTTT AAAAGTGGCT
    GATTGCAGAG GACATTTAAG TTGGGTTCAT TGTGTGATGT TTTCTCCCGA TGGATCATCA
    TTTTTGACAT CTTCTGATGA CCAGACAATC AGGCTCTGGG AGACCAAAAA AGTATGTAAG
    AACTCTGCTA TAGTGTTAAA GCAAGAAATA GATGTTGTGT TTCAAGAAAA TGAAGTGATG
    GTTCTTGCAG TTGACAATAT AAAACGTCTG CAGCTCATTA ATGGAAAAAC AGGTCAGATT
    GATTATCTGA TTGAAGCTCA GGTTAGTTGC TGTTGCCTAA GTCCACATCT TCAGTACATT
    GCATTTGGAG GTGAAGATGG AGCCGTTGAG ATTTTAGAAC TCCTAAACAA CAGAATCTTC
    CAATCCAGGA TTGGGCACAA GAAAACTGTG CGGCACATCC AGTTCACAGA TGATGGGAAG
    ACTCTTATTT CAAGCTCTGA TGATTCTTCA ATTCAGGTAT GGAATTGGCA GTCAGAGGAA
    TATGTCTTCC TACAAGCCCA TCAGGAAACT GTGAAAGACT TTAAGCTCCT GAAAAATTCA
    AAACTGCTTT CCTGGTCATT TGATGGAACA GTAAAGATAT GGAGTATTAT TACTGGAAGA
    ATAGAAAAAG ACTTTGTCTG TCACCAGGAT ACAGTACTTT CTTGTGATAT TTCTCCTGAT
    GCTACCAAGT TTTCATCTAC ATCTGCAGAT AAGACTGCGA AGATTTGGAG TTTTGAACTT
    CTTTCACCTC TTCATGAATT GAGGGGCCAC AAGGGCTGTG TGCGCTGCTC TGTCTTCTCT
    GTGGACAGTA CCCTGTTGGC GACTGGAGAT GACAATGGAG AAATCAGGAT ATGGAATGTC
    TCAAATGGTG AGCTGCTTCA TTTGTGTGCT CCGATTTTGG TAGAAGAAGG AGCTGCTACC
    CATGGAGGCT GGGTGACTGA CCTTTGCTTT TCTCCGGATA GCAAAATGCT TGTCTCTGCT
    GGAGGATATC TTAAGGCGAT CACCAGCCCA GGGACACCGT TCCACCCGTC TGTTTCCCTA
    CATGCTGCCA ACACCTATGT CACTAAGTCT TGGAATGGCT GTTCAAGAAG CCACAAAAAA
    CGGGAACACC ATGCAATGCT AAAGAAGGCT GGGCAGATTC AGCAGGAAGA GGAACTGCCA
    GCCCGTGAGC TGGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe293658
    Clone ID Related Accession (Same CDS sequence) XM_022403389.1
    Accession Version XM_022403389.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3198bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product apoptotic protease-activating factor 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAATTGC AGAATCTTTG CACACGGTTG GATCAGGATG AGAATTTCTC TCAGAGGCCT 
    CCACTCAATA TTGAAGAAGC TAAAGACCGT CTTCGCCTTC TTATGCTGCG CAAACACCCA
    AGGTCTCTGT TGATCTTGGA TGATATTTGG GATTCCTGGA TATTAAAAGC TTTTGACAAT
    CAGTGTCAGA TTCTTCTTAC AACCAGAGAC AAGAGCGTTA CAGATTCAGT AATGGGTCCT
    AAATATATAG TCGCTGTGGA GAGTGACTTA GGAAAAGAAA AAGGACTTGA AATTCTGTCC
    CTCTTTGTTA ATATGAAGAA AGCAGATTTG CCAGAGCAAG CTCATAGTAT TATAAAAGAA
    TGTAAAGGTT CTCCTCTCGT AGTGTCTTTA ATTGGTGCAC TTTTACGTGA TTTTCCTAAC
    CGTTGGGATT ACTACCTCAG GCAACTTCAG AATAAGCAAT TTAAGAGAAT AAGGAAATCT
    TCATTTTATG ATTATGAGGC TCTGGATGAA GCCATGTCTA TAAGTGTTGA AATGCTCAGA
    GAGGACATCA AAGATTACTA CACAGATCTT TCCATCCTTC AGAAGGATGT TAAGGTGCCT
    ACAAAGGTGT TATGTATTCT CTGGGACATG GAAACTGAAG AAGTTGAAGA TATTCTGCAG
    GAGTTTGTTA ATAAGTCTCT CTTATTCTGT GATCGGAATG GAAAATCATT TCGTTATTAT
    TTACATGATC TTCAAGTAGA TTTCCTTATA GAGAAGAATC GCAATCAACT TCAGGATCTA
    CATAAGAAGA TAGTCACTCA GTTCCAGAAA CATTACCAGC CACATACTCT TTCACCAGAT
    CAGGAGGACT GCATGTACTG GTACAATTTT CTGGCCTACC ACATGGCTAG TGCCAGTATG
    CACAAAGAAC TTTGTGCTTT AATGTTTTCC TTGGATTGGA TTAAAGCAAA AACTGAACTC
    GTAGGCCCTG CTCATCTGAT TCATGAATTT GTGGAATACA GGCATATATT GGATGAAAAG
    GATTGTGCAG TCTGTGAAAA TTTCCAGGAA TTTTTATCTT TAAATGGACA TCTTCTTGGA
    CGACAGCCAT TTCCTAATAT TGTACAACTG GGTCTCTGTG AACCAGAAAC TTCAGAAGTT
    TATCAGCAAG CTAAGCTGCA GGCCAAGCAG GAGGTCGATC ACGGAATGCT TTACCTGGAG
    TGGATAAACA AAAAAAACAT CAAGAATCTT TCCCGCTTAG TTGTCCGCCC CCATACAGAT
    GCTGTTTACC ATGCTTGCTT TTCTGAGGAT GGTCAAAGAA TAGCTTCTTG TGGAGTTGAT
    AAAACCTTAC AGGTGTTCAA AGCTGAAACA GGAGAGAAAC TGCTAGAAAT CAAGGCTCAT
    GAAGATGAAG TGCTTTGTTG TGCATTCTCT ACAGATGACA GATTTATAGC AACCTGCTCA
    GTGGATAAAA AAGTGAAGAT TTGGAATTCT ATGACAGGGG GACTAGTACA TGTCTATGAT
    GAGCACTCAG AGCAAGTCAA CTGCTGCCAC TTTACCAACA ACAGTCATTA CCTTCTCTTA
    GCCACTGCAT CAAGTGACTG CTTCCTCAAA CTCTGGGATT TGAATCAAAA AGAATGTCGA
    AATACCATGT TTGGCCATAC AAATTCAGTC AATCACTGCA GATTTTCACC AGATGATAAG
    CTTTTGGCTA GTTGTTCAGC TGATGGAACA TTAAAGCTTT GGGATGTAAA ATCAGCAAAT
    GAGAGGAAAA GCATTAATGT GAAGCAGTTC TTCCTAAATT CAGAGGAGCC TCAAGAGGAT
    ATGGAAGTGA TAGTGAAATG TTGTTCATGG TCTGCTGATG GCACTAGGAT AATGGTGGCA
    GCAAAAAATA AAATCTTTCT ATTTGACATT CATACTGGTG GCTTATTGGC AGAAATCCAT
    ACAGGCCATC ACAGCACCAT CCAGTACTGT GACTTCTCCC CCCAAAATCA TTTGGCAGTA
    GTGGCTTTGT CCCAGTGCTG TGTGGAGTTG TGGAACATGG ACTCCTGTTT AAAAGTGGCT
    GATTGCAGAG GACATTTAAG TTGGGTTCAT TGTGTGATGT TTTCTCCCGA TGGATCATCA
    TTTTTGACAT CTTCTGATGA CCAGACAATC AGGCTCTGGG AGACCAAAAA AGTATGTAAG
    AACTCTGCTA TAGTGTTAAA GCAAGAAATA GATGTTGTGT TTCAAGAAAA TGAAGTGATG
    GTTCTTGCAG TTGACAATAT AAAACGTCTG CAGCTCATTA ATGGAAAAAC AGGTCAGATT
    GATTATCTGA TTGAAGCTCA GGTTAGTTGC TGTTGCCTAA GTCCACATCT TCAGTACATT
    GCATTTGGAG GTGAAGATGG AGCCGTTGAG ATTTTAGAAC TCCTAAACAA CAGAATCTTC
    CAATCCAGGA TTGGGCACAA GAAAACTGTG CGGCACATCC AGTTCACAGA TGATGGGAAG
    ACTCTTATTT CAAGCTCTGA TGATTCTTCA ATTCAGGTAT GGAATTGGCA GTCAGAGGAA
    TATGTCTTCC TACAAGCCCA TCAGGAAACT GTGAAAGACT TTAAGCTCCT GAAAAATTCA
    AAACTGCTTT CCTGGTCATT TGATGGAACA GTAAAGATAT GGAGTATTAT TACTGGAAGA
    ATAGAAAAAG ACTTTGTCTG TCACCAGGAT ACAGTACTTT CTTGTGATAT TTCTCCTGAT
    GCTACCAAGT TTTCATCTAC ATCTGCAGAT AAGACTGCGA AGATTTGGAG TTTTGAACTT
    CTTTCACCTC TTCATGAATT GAGGGGCCAC AAGGGCTGTG TGCGCTGCTC TGTCTTCTCT
    GTGGACAGTA CCCTGTTGGC GACTGGAGAT GACAATGGAG AAATCAGGAT ATGGAATGTC
    TCAAATGGTG AGCTGCTTCA TTTGTGTGCT CCGATTTTGG TAGAAGAAGG AGCTGCTACC
    CATGGAGGCT GGGTGACTGA CCTTTGCTTT TCTCCGGATA GCAAAATGCT TGTCTCTGCT
    GGAGGATATC TTAAGGCGAT CACCAGCCCA GGGACACCGT TCCACCCGTC TGTTTCCCTA
    CATGCTGCCA ACACCTATGT CACTAAGTCT TGGAATGGCT GTTCAAGAAG CCACAAAAAA
    CGGGAACACC ATGCAATGCT AAAGAAGGCT GGGCAGATTC AGCAGGAAGA GGAACTGCCA
    GCCCGTGAGC TGGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022259097.1
    CDS198..3395
    Translation

    Target ORF information:

    RefSeq Version XM_022403389.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022403389.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAATTGC AGAATCTTTG CACACGGTTG GATCAGGATG AGAATTTCTC TCAGAGGCCT 
    CCACTCAATA TTGAAGAAGC TAAAGACCGT CTTCGCCTTC TTATGCTGCG CAAACACCCA
    AGGTCTCTGT TGATCTTGGA TGATATTTGG GATTCCTGGA TATTAAAAGC TTTTGACAAT
    CAGTGTCAGA TTCTTCTTAC AACCAGAGAC AAGAGCGTTA CAGATTCAGT AATGGGTCCT
    AAATATATAG TCGCTGTGGA GAGTGACTTA GGAAAAGAAA AAGGACTTGA AATTCTGTCC
    CTCTTTGTTA ATATGAAGAA AGCAGATTTG CCAGAGCAAG CTCATAGTAT TATAAAAGAA
    TGTAAAGGTT CTCCTCTCGT AGTGTCTTTA ATTGGTGCAC TTTTACGTGA TTTTCCTAAC
    CGTTGGGATT ACTACCTCAG GCAACTTCAG AATAAGCAAT TTAAGAGAAT AAGGAAATCT
    TCATTTTATG ATTATGAGGC TCTGGATGAA GCCATGTCTA TAAGTGTTGA AATGCTCAGA
    GAGGACATCA AAGATTACTA CACAGATCTT TCCATCCTTC AGAAGGATGT TAAGGTGCCT
    ACAAAGGTGT TATGTATTCT CTGGGACATG GAAACTGAAG AAGTTGAAGA TATTCTGCAG
    GAGTTTGTTA ATAAGTCTCT CTTATTCTGT GATCGGAATG GAAAATCATT TCGTTATTAT
    TTACATGATC TTCAAGTAGA TTTCCTTATA GAGAAGAATC GCAATCAACT TCAGGATCTA
    CATAAGAAGA TAGTCACTCA GTTCCAGAAA CATTACCAGC CACATACTCT TTCACCAGAT
    CAGGAGGACT GCATGTACTG GTACAATTTT CTGGCCTACC ACATGGCTAG TGCCAGTATG
    CACAAAGAAC TTTGTGCTTT AATGTTTTCC TTGGATTGGA TTAAAGCAAA AACTGAACTC
    GTAGGCCCTG CTCATCTGAT TCATGAATTT GTGGAATACA GGCATATATT GGATGAAAAG
    GATTGTGCAG TCTGTGAAAA TTTCCAGGAA TTTTTATCTT TAAATGGACA TCTTCTTGGA
    CGACAGCCAT TTCCTAATAT TGTACAACTG GGTCTCTGTG AACCAGAAAC TTCAGAAGTT
    TATCAGCAAG CTAAGCTGCA GGCCAAGCAG GAGGTCGATC ACGGAATGCT TTACCTGGAG
    TGGATAAACA AAAAAAACAT CAAGAATCTT TCCCGCTTAG TTGTCCGCCC CCATACAGAT
    GCTGTTTACC ATGCTTGCTT TTCTGAGGAT GGTCAAAGAA TAGCTTCTTG TGGAGTTGAT
    AAAACCTTAC AGGTGTTCAA AGCTGAAACA GGAGAGAAAC TGCTAGAAAT CAAGGCTCAT
    GAAGATGAAG TGCTTTGTTG TGCATTCTCT ACAGATGACA GATTTATAGC AACCTGCTCA
    GTGGATAAAA AAGTGAAGAT TTGGAATTCT ATGACAGGGG GACTAGTACA TGTCTATGAT
    GAGCACTCAG AGCAAGTCAA CTGCTGCCAC TTTACCAACA ACAGTCATTA CCTTCTCTTA
    GCCACTGCAT CAAGTGACTG CTTCCTCAAA CTCTGGGATT TGAATCAAAA AGAATGTCGA
    AATACCATGT TTGGCCATAC AAATTCAGTC AATCACTGCA GATTTTCACC AGATGATAAG
    CTTTTGGCTA GTTGTTCAGC TGATGGAACA TTAAAGCTTT GGGATGTAAA ATCAGCAAAT
    GAGAGGAAAA GCATTAATGT GAAGCAGTTC TTCCTAAATT CAGAGGAGCC TCAAGAGGAT
    ATGGAAGTGA TAGTGAAATG TTGTTCATGG TCTGCTGATG GCACTAGGAT AATGGTGGCA
    GCAAAAAATA AAATCTTTCT ATTTGACATT CATACTGGTG GCTTATTGGC AGAAATCCAT
    ACAGGCCATC ACAGCACCAT CCAGTACTGT GACTTCTCCC CCCAAAATCA TTTGGCAGTA
    GTGGCTTTGT CCCAGTGCTG TGTGGAGTTG TGGAACATGG ACTCCTGTTT AAAAGTGGCT
    GATTGCAGAG GACATTTAAG TTGGGTTCAT TGTGTGATGT TTTCTCCCGA TGGATCATCA
    TTTTTGACAT CTTCTGATGA CCAGACAATC AGGCTCTGGG AGACCAAAAA AGTATGTAAG
    AACTCTGCTA TAGTGTTAAA GCAAGAAATA GATGTTGTGT TTCAAGAAAA TGAAGTGATG
    GTTCTTGCAG TTGACAATAT AAAACGTCTG CAGCTCATTA ATGGAAAAAC AGGTCAGATT
    GATTATCTGA TTGAAGCTCA GGTTAGTTGC TGTTGCCTAA GTCCACATCT TCAGTACATT
    GCATTTGGAG GTGAAGATGG AGCCGTTGAG ATTTTAGAAC TCCTAAACAA CAGAATCTTC
    CAATCCAGGA TTGGGCACAA GAAAACTGTG CGGCACATCC AGTTCACAGA TGATGGGAAG
    ACTCTTATTT CAAGCTCTGA TGATTCTTCA ATTCAGGTAT GGAATTGGCA GTCAGAGGAA
    TATGTCTTCC TACAAGCCCA TCAGGAAACT GTGAAAGACT TTAAGCTCCT GAAAAATTCA
    AAACTGCTTT CCTGGTCATT TGATGGAACA GTAAAGATAT GGAGTATTAT TACTGGAAGA
    ATAGAAAAAG ACTTTGTCTG TCACCAGGAT ACAGTACTTT CTTGTGATAT TTCTCCTGAT
    GCTACCAAGT TTTCATCTAC ATCTGCAGAT AAGACTGCGA AGATTTGGAG TTTTGAACTT
    CTTTCACCTC TTCATGAATT GAGGGGCCAC AAGGGCTGTG TGCGCTGCTC TGTCTTCTCT
    GTGGACAGTA CCCTGTTGGC GACTGGAGAT GACAATGGAG AAATCAGGAT ATGGAATGTC
    TCAAATGGTG AGCTGCTTCA TTTGTGTGCT CCGATTTTGG TAGAAGAAGG AGCTGCTACC
    CATGGAGGCT GGGTGACTGA CCTTTGCTTT TCTCCGGATA GCAAAATGCT TGTCTCTGCT
    GGAGGATATC TTAAGGCGAT CACCAGCCCA GGGACACCGT TCCACCCGTC TGTTTCCCTA
    CATGCTGCCA ACACCTATGT CACTAAGTCT TGGAATGGCT GTTCAAGAAG CCACAAAAAA
    CGGGAACACC ATGCAATGCT AAAGAAGGCT GGGCAGATTC AGCAGGAAGA GGAACTGCCA
    GCCCGTGAGC TGGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe293659
    Clone ID Related Accession (Same CDS sequence) XM_022403390.1
    Accession Version XM_022403390.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3099bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product apoptotic protease-activating factor 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCTCAGGA AGTACTTCTT AAGGTCTCTG TTGATCTTGG ATGATATTTG GGATTCCTGG 
    ATATTAAAAG CTTTTGACAA TCAGTGTCAG ATTCTTCTTA CAACCAGAGA CAAGAGCGTT
    ACAGATTCAG TAATGGGTCC TAAATATATA GTCGCTGTGG AGAGTGACTT AGGAAAAGAA
    AAAGGACTTG AAATTCTGTC CCTCTTTGTT AATATGAAGA AAGCAGATTT GCCAGAGCAA
    GCTCATAGTA TTATAAAAGA ATGTAAAGGT TCTCCTCTCG TAGTGTCTTT AATTGGTGCA
    CTTTTACGTG ATTTTCCTAA CCGTTGGGAT TACTACCTCA GGCAACTTCA GAATAAGCAA
    TTTAAGAGAA TAAGGAAATC TTCATTTTAT GATTATGAGG CTCTGGATGA AGCCATGTCT
    ATAAGTGTTG AAATGCTCAG AGAGGACATC AAAGATTACT ACACAGATCT TTCCATCCTT
    CAGAAGGATG TTAAGGTGCC TACAAAGGTG TTATGTATTC TCTGGGACAT GGAAACTGAA
    GAAGTTGAAG ATATTCTGCA GGAGTTTGTT AATAAGTCTC TCTTATTCTG TGATCGGAAT
    GGAAAATCAT TTCGTTATTA TTTACATGAT CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT
    CGCAATCAAC TTCAGGATCT ACATAAGAAG ATAGTCACTC AGTTCCAGAA ACATTACCAG
    CCACATACTC TTTCACCAGA TCAGGAGGAC TGCATGTACT GGTACAATTT TCTGGCCTAC
    CACATGGCTA GTGCCAGTAT GCACAAAGAA CTTTGTGCTT TAATGTTTTC CTTGGATTGG
    ATTAAAGCAA AAACTGAACT CGTAGGCCCT GCTCATCTGA TTCATGAATT TGTGGAATAC
    AGGCATATAT TGGATGAAAA GGATTGTGCA GTCTGTGAAA ATTTCCAGGA ATTTTTATCT
    TTAAATGGAC ATCTTCTTGG ACGACAGCCA TTTCCTAATA TTGTACAACT GGGTCTCTGT
    GAACCAGAAA CTTCAGAAGT TTATCAGCAA GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT
    CACGGAATGC TTTACCTGGA GTGGATAAAC AAAAAAAACA TCAAGAATCT TTCCCGCTTA
    GTTGTCCGCC CCCATACAGA TGCTGTTTAC CATGCTTGCT TTTCTGAGGA TGGTCAAAGA
    ATAGCTTCTT GTGGAGTTGA TAAAACCTTA CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA
    CTGCTAGAAA TCAAGGCTCA TGAAGATGAA GTGCTTTGTT GTGCATTCTC TACAGATGAC
    AGATTTATAG CAACCTGCTC AGTGGATAAA AAAGTGAAGA TTTGGAATTC TATGACAGGG
    GGACTAGTAC ATGTCTATGA TGAGCACTCA GAGCAAGTCA ACTGCTGCCA CTTTACCAAC
    AACAGTCATT ACCTTCTCTT AGCCACTGCA TCAAGTGACT GCTTCCTCAA ACTCTGGGAT
    TTGAATCAAA AAGAATGTCG AAATACCATG TTTGGCCATA CAAATTCAGT CAATCACTGC
    AGATTTTCAC CAGATGATAA GCTTTTGGCT AGTTGTTCAG CTGATGGAAC ATTAAAGCTT
    TGGGATGTAA AATCAGCAAA TGAGAGGAAA AGCATTAATG TGAAGCAGTT CTTCCTAAAT
    TCAGAGGAGC CTCAAGAGGA TATGGAAGTG ATAGTGAAAT GTTGTTCATG GTCTGCTGAT
    GGCACTAGGA TAATGGTGGC AGCAAAAAAT AAAATCTTTC TATTTGACAT TCATACTGGT
    GGCTTATTGG CAGAAATCCA TACAGGCCAT CACAGCACCA TCCAGTACTG TGACTTCTCC
    CCCCAAAATC ATTTGGCAGT AGTGGCTTTG TCCCAGTGCT GTGTGGAGTT GTGGAACATG
    GACTCCTGTT TAAAAGTGGC TGATTGCAGA GGACATTTAA GTTGGGTTCA TTGTGTGATG
    TTTTCTCCCG ATGGATCATC ATTTTTGACA TCTTCTGATG ACCAGACAAT CAGGCTCTGG
    GAGACCAAAA AAGTATGTAA GAACTCTGCT ATAGTGTTAA AGCAAGAAAT AGATGTTGTG
    TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA GTTGACAATA TAAAACGTCT GCAGCTCATT
    AATGGAAAAA CAGGTCAGAT TGATTATCTG ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA
    AGTCCACATC TTCAGTACAT TGCATTTGGA GGTGAAGATG GAGCCGTTGA GATTTTAGAA
    CTCCTAAACA ACAGAATCTT CCAATCCAGG ATTGGGCACA AGAAAACTGT GCGGCACATC
    CAGTTCACAG ATGATGGGAA GACTCTTATT TCAAGCTCTG ATGATTCTTC AATTCAGGTA
    TGGAATTGGC AGTCAGAGGA ATATGTCTTC CTACAAGCCC ATCAGGAAAC TGTGAAAGAC
    TTTAAGCTCC TGAAAAATTC AAAACTGCTT TCCTGGTCAT TTGATGGAAC AGTAAAGATA
    TGGAGTATTA TTACTGGAAG AATAGAAAAA GACTTTGTCT GTCACCAGGA TACAGTACTT
    TCTTGTGATA TTTCTCCTGA TGCTACCAAG TTTTCATCTA CATCTGCAGA TAAGACTGCG
    AAGATTTGGA GTTTTGAACT TCTTTCACCT CTTCATGAAT TGAGGGGCCA CAAGGGCTGT
    GTGCGCTGCT CTGTCTTCTC TGTGGACAGT ACCCTGTTGG CGACTGGAGA TGACAATGGA
    GAAATCAGGA TATGGAATGT CTCAAATGGT GAGCTGCTTC ATTTGTGTGC TCCGATTTTG
    GTAGAAGAAG GAGCTGCTAC CCATGGAGGC TGGGTGACTG ACCTTTGCTT TTCTCCGGAT
    AGCAAAATGC TTGTCTCTGC TGGAGGATAT CTTAAGGCGA TCACCAGCCC AGGGACACCG
    TTCCACCCGT CTGTTTCCCT ACATGCTGCC AACACCTATG TCACTAAGTC TTGGAATGGC
    TGTTCAAGAA GCCACAAAAA ACGGGAACAC CATGCAATGC TAAAGAAGGC TGGGCAGATT
    CAGCAGGAAG AGGAACTGCC AGCCCGTGAG CTGGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022259098.1
    CDS101..3199
    Translation

    Target ORF information:

    RefSeq Version XM_022403390.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022403390.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCTCAGGA AGTACTTCTT AAGGTCTCTG TTGATCTTGG ATGATATTTG GGATTCCTGG 
    ATATTAAAAG CTTTTGACAA TCAGTGTCAG ATTCTTCTTA CAACCAGAGA CAAGAGCGTT
    ACAGATTCAG TAATGGGTCC TAAATATATA GTCGCTGTGG AGAGTGACTT AGGAAAAGAA
    AAAGGACTTG AAATTCTGTC CCTCTTTGTT AATATGAAGA AAGCAGATTT GCCAGAGCAA
    GCTCATAGTA TTATAAAAGA ATGTAAAGGT TCTCCTCTCG TAGTGTCTTT AATTGGTGCA
    CTTTTACGTG ATTTTCCTAA CCGTTGGGAT TACTACCTCA GGCAACTTCA GAATAAGCAA
    TTTAAGAGAA TAAGGAAATC TTCATTTTAT GATTATGAGG CTCTGGATGA AGCCATGTCT
    ATAAGTGTTG AAATGCTCAG AGAGGACATC AAAGATTACT ACACAGATCT TTCCATCCTT
    CAGAAGGATG TTAAGGTGCC TACAAAGGTG TTATGTATTC TCTGGGACAT GGAAACTGAA
    GAAGTTGAAG ATATTCTGCA GGAGTTTGTT AATAAGTCTC TCTTATTCTG TGATCGGAAT
    GGAAAATCAT TTCGTTATTA TTTACATGAT CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT
    CGCAATCAAC TTCAGGATCT ACATAAGAAG ATAGTCACTC AGTTCCAGAA ACATTACCAG
    CCACATACTC TTTCACCAGA TCAGGAGGAC TGCATGTACT GGTACAATTT TCTGGCCTAC
    CACATGGCTA GTGCCAGTAT GCACAAAGAA CTTTGTGCTT TAATGTTTTC CTTGGATTGG
    ATTAAAGCAA AAACTGAACT CGTAGGCCCT GCTCATCTGA TTCATGAATT TGTGGAATAC
    AGGCATATAT TGGATGAAAA GGATTGTGCA GTCTGTGAAA ATTTCCAGGA ATTTTTATCT
    TTAAATGGAC ATCTTCTTGG ACGACAGCCA TTTCCTAATA TTGTACAACT GGGTCTCTGT
    GAACCAGAAA CTTCAGAAGT TTATCAGCAA GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT
    CACGGAATGC TTTACCTGGA GTGGATAAAC AAAAAAAACA TCAAGAATCT TTCCCGCTTA
    GTTGTCCGCC CCCATACAGA TGCTGTTTAC CATGCTTGCT TTTCTGAGGA TGGTCAAAGA
    ATAGCTTCTT GTGGAGTTGA TAAAACCTTA CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA
    CTGCTAGAAA TCAAGGCTCA TGAAGATGAA GTGCTTTGTT GTGCATTCTC TACAGATGAC
    AGATTTATAG CAACCTGCTC AGTGGATAAA AAAGTGAAGA TTTGGAATTC TATGACAGGG
    GGACTAGTAC ATGTCTATGA TGAGCACTCA GAGCAAGTCA ACTGCTGCCA CTTTACCAAC
    AACAGTCATT ACCTTCTCTT AGCCACTGCA TCAAGTGACT GCTTCCTCAA ACTCTGGGAT
    TTGAATCAAA AAGAATGTCG AAATACCATG TTTGGCCATA CAAATTCAGT CAATCACTGC
    AGATTTTCAC CAGATGATAA GCTTTTGGCT AGTTGTTCAG CTGATGGAAC ATTAAAGCTT
    TGGGATGTAA AATCAGCAAA TGAGAGGAAA AGCATTAATG TGAAGCAGTT CTTCCTAAAT
    TCAGAGGAGC CTCAAGAGGA TATGGAAGTG ATAGTGAAAT GTTGTTCATG GTCTGCTGAT
    GGCACTAGGA TAATGGTGGC AGCAAAAAAT AAAATCTTTC TATTTGACAT TCATACTGGT
    GGCTTATTGG CAGAAATCCA TACAGGCCAT CACAGCACCA TCCAGTACTG TGACTTCTCC
    CCCCAAAATC ATTTGGCAGT AGTGGCTTTG TCCCAGTGCT GTGTGGAGTT GTGGAACATG
    GACTCCTGTT TAAAAGTGGC TGATTGCAGA GGACATTTAA GTTGGGTTCA TTGTGTGATG
    TTTTCTCCCG ATGGATCATC ATTTTTGACA TCTTCTGATG ACCAGACAAT CAGGCTCTGG
    GAGACCAAAA AAGTATGTAA GAACTCTGCT ATAGTGTTAA AGCAAGAAAT AGATGTTGTG
    TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA GTTGACAATA TAAAACGTCT GCAGCTCATT
    AATGGAAAAA CAGGTCAGAT TGATTATCTG ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA
    AGTCCACATC TTCAGTACAT TGCATTTGGA GGTGAAGATG GAGCCGTTGA GATTTTAGAA
    CTCCTAAACA ACAGAATCTT CCAATCCAGG ATTGGGCACA AGAAAACTGT GCGGCACATC
    CAGTTCACAG ATGATGGGAA GACTCTTATT TCAAGCTCTG ATGATTCTTC AATTCAGGTA
    TGGAATTGGC AGTCAGAGGA ATATGTCTTC CTACAAGCCC ATCAGGAAAC TGTGAAAGAC
    TTTAAGCTCC TGAAAAATTC AAAACTGCTT TCCTGGTCAT TTGATGGAAC AGTAAAGATA
    TGGAGTATTA TTACTGGAAG AATAGAAAAA GACTTTGTCT GTCACCAGGA TACAGTACTT
    TCTTGTGATA TTTCTCCTGA TGCTACCAAG TTTTCATCTA CATCTGCAGA TAAGACTGCG
    AAGATTTGGA GTTTTGAACT TCTTTCACCT CTTCATGAAT TGAGGGGCCA CAAGGGCTGT
    GTGCGCTGCT CTGTCTTCTC TGTGGACAGT ACCCTGTTGG CGACTGGAGA TGACAATGGA
    GAAATCAGGA TATGGAATGT CTCAAATGGT GAGCTGCTTC ATTTGTGTGC TCCGATTTTG
    GTAGAAGAAG GAGCTGCTAC CCATGGAGGC TGGGTGACTG ACCTTTGCTT TTCTCCGGAT
    AGCAAAATGC TTGTCTCTGC TGGAGGATAT CTTAAGGCGA TCACCAGCCC AGGGACACCG
    TTCCACCCGT CTGTTTCCCT ACATGCTGCC AACACCTATG TCACTAAGTC TTGGAATGGC
    TGTTCAAGAA GCCACAAAAA ACGGGAACAC CATGCAATGC TAAAGAAGGC TGGGCAGATT
    CAGCAGGAAG AGGAACTGCC AGCCCGTGAG CTGGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40116
    Clone ID Related Accession (Same CDS sequence) XM_014119289.1 , XM_014119291.1 , XM_014119294.1 , XM_014119288.1 , XM_014119290.1 , XM_014119292.1 , XM_014119293.1 , XM_014119295.1 , XM_014119285.1 , XM_014119286.1 , XM_014119287.1
    Accession Version XM_014119289.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product apoptotic protease-activating factor 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGATA TGGAGTATTA TTACTGGAAG AATAGAAAAA
    GACTTTGTCT GTCACCAGGA TACAGTACTT TCTTGTGATA TTTCTCCTGA TGCTACCAAG
    TTTTCATCTA CATCTGCAGA TAAGACTGCG AAGATTTGGA GTTTTGAACT TCTTTCACCT
    CTTCATGAAT TGAGGGGCCA CAAGGGCTGT GTGCGCTGCT CTGTCTTCTC TGTGGACAGT
    ACCCTGTTGG CGACTGGAGA TGACAATGGA GAAATCAGGA TATGGAATGT CTCAAATGGT
    GAGCTGCTTC ATTTGTGTGC TCCGATTTTG GTAGAAGAAG GAGCTGCTAC CCATGGAGGC
    TGGGTGACTG ACCTTTGCTT TTCTCCGGAT AGCAAAATGC TTGTCTCTGC TGGAGGATAT
    CTTAAGGCGA TCACCAGCCC AGGGACACCG TTCCACCCGT CTGTTTCCCT ACATGCTGCC
    AACACCTATG GCTTGGGATA CATGAGGTGC TCAACAAATG TTTGTTGGAA GAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974764.1
    CDS172..3888
    Translation

    Target ORF information:

    RefSeq Version XM_014119289.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014119289.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGATA TGGAGTATTA TTACTGGAAG AATAGAAAAA
    GACTTTGTCT GTCACCAGGA TACAGTACTT TCTTGTGATA TTTCTCCTGA TGCTACCAAG
    TTTTCATCTA CATCTGCAGA TAAGACTGCG AAGATTTGGA GTTTTGAACT TCTTTCACCT
    CTTCATGAAT TGAGGGGCCA CAAGGGCTGT GTGCGCTGCT CTGTCTTCTC TGTGGACAGT
    ACCCTGTTGG CGACTGGAGA TGACAATGGA GAAATCAGGA TATGGAATGT CTCAAATGGT
    GAGCTGCTTC ATTTGTGTGC TCCGATTTTG GTAGAAGAAG GAGCTGCTAC CCATGGAGGC
    TGGGTGACTG ACCTTTGCTT TTCTCCGGAT AGCAAAATGC TTGTCTCTGC TGGAGGATAT
    CTTAAGGCGA TCACCAGCCC AGGGACACCG TTCCACCCGT CTGTTTCCCT ACATGCTGCC
    AACACCTATG GCTTGGGATA CATGAGGTGC TCAACAAATG TTTGTTGGAA GAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40116
    Clone ID Related Accession (Same CDS sequence) XM_014119289.1 , XM_014119291.1 , XM_014119294.1 , XM_014119288.1 , XM_014119290.1 , XM_014119292.1 , XM_014119293.1 , XM_014119295.1 , XM_014119285.1 , XM_014119286.1 , XM_014119287.1
    Accession Version XM_014119291.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product apoptotic protease-activating factor 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGATA TGGAGTATTA TTACTGGAAG AATAGAAAAA
    GACTTTGTCT GTCACCAGGA TACAGTACTT TCTTGTGATA TTTCTCCTGA TGCTACCAAG
    TTTTCATCTA CATCTGCAGA TAAGACTGCG AAGATTTGGA GTTTTGAACT TCTTTCACCT
    CTTCATGAAT TGAGGGGCCA CAAGGGCTGT GTGCGCTGCT CTGTCTTCTC TGTGGACAGT
    ACCCTGTTGG CGACTGGAGA TGACAATGGA GAAATCAGGA TATGGAATGT CTCAAATGGT
    GAGCTGCTTC ATTTGTGTGC TCCGATTTTG GTAGAAGAAG GAGCTGCTAC CCATGGAGGC
    TGGGTGACTG ACCTTTGCTT TTCTCCGGAT AGCAAAATGC TTGTCTCTGC TGGAGGATAT
    CTTAAGGCGA TCACCAGCCC AGGGACACCG TTCCACCCGT CTGTTTCCCT ACATGCTGCC
    AACACCTATG GCTTGGGATA CATGAGGTGC TCAACAAATG TTTGTTGGAA GAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974766.1
    CDS172..3888
    Translation

    Target ORF information:

    RefSeq Version XM_014119291.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014119291.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGATA TGGAGTATTA TTACTGGAAG AATAGAAAAA
    GACTTTGTCT GTCACCAGGA TACAGTACTT TCTTGTGATA TTTCTCCTGA TGCTACCAAG
    TTTTCATCTA CATCTGCAGA TAAGACTGCG AAGATTTGGA GTTTTGAACT TCTTTCACCT
    CTTCATGAAT TGAGGGGCCA CAAGGGCTGT GTGCGCTGCT CTGTCTTCTC TGTGGACAGT
    ACCCTGTTGG CGACTGGAGA TGACAATGGA GAAATCAGGA TATGGAATGT CTCAAATGGT
    GAGCTGCTTC ATTTGTGTGC TCCGATTTTG GTAGAAGAAG GAGCTGCTAC CCATGGAGGC
    TGGGTGACTG ACCTTTGCTT TTCTCCGGAT AGCAAAATGC TTGTCTCTGC TGGAGGATAT
    CTTAAGGCGA TCACCAGCCC AGGGACACCG TTCCACCCGT CTGTTTCCCT ACATGCTGCC
    AACACCTATG GCTTGGGATA CATGAGGTGC TCAACAAATG TTTGTTGGAA GAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40116
    Clone ID Related Accession (Same CDS sequence) XM_014119289.1 , XM_014119291.1 , XM_014119294.1 , XM_014119288.1 , XM_014119290.1 , XM_014119292.1 , XM_014119293.1 , XM_014119295.1 , XM_014119285.1 , XM_014119286.1 , XM_014119287.1
    Accession Version XM_014119294.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product apoptotic protease-activating factor 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGATA TGGAGTATTA TTACTGGAAG AATAGAAAAA
    GACTTTGTCT GTCACCAGGA TACAGTACTT TCTTGTGATA TTTCTCCTGA TGCTACCAAG
    TTTTCATCTA CATCTGCAGA TAAGACTGCG AAGATTTGGA GTTTTGAACT TCTTTCACCT
    CTTCATGAAT TGAGGGGCCA CAAGGGCTGT GTGCGCTGCT CTGTCTTCTC TGTGGACAGT
    ACCCTGTTGG CGACTGGAGA TGACAATGGA GAAATCAGGA TATGGAATGT CTCAAATGGT
    GAGCTGCTTC ATTTGTGTGC TCCGATTTTG GTAGAAGAAG GAGCTGCTAC CCATGGAGGC
    TGGGTGACTG ACCTTTGCTT TTCTCCGGAT AGCAAAATGC TTGTCTCTGC TGGAGGATAT
    CTTAAGGCGA TCACCAGCCC AGGGACACCG TTCCACCCGT CTGTTTCCCT ACATGCTGCC
    AACACCTATG GCTTGGGATA CATGAGGTGC TCAACAAATG TTTGTTGGAA GAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974769.1
    CDS172..3888
    Translation

    Target ORF information:

    RefSeq Version XM_014119294.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014119294.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGATA TGGAGTATTA TTACTGGAAG AATAGAAAAA
    GACTTTGTCT GTCACCAGGA TACAGTACTT TCTTGTGATA TTTCTCCTGA TGCTACCAAG
    TTTTCATCTA CATCTGCAGA TAAGACTGCG AAGATTTGGA GTTTTGAACT TCTTTCACCT
    CTTCATGAAT TGAGGGGCCA CAAGGGCTGT GTGCGCTGCT CTGTCTTCTC TGTGGACAGT
    ACCCTGTTGG CGACTGGAGA TGACAATGGA GAAATCAGGA TATGGAATGT CTCAAATGGT
    GAGCTGCTTC ATTTGTGTGC TCCGATTTTG GTAGAAGAAG GAGCTGCTAC CCATGGAGGC
    TGGGTGACTG ACCTTTGCTT TTCTCCGGAT AGCAAAATGC TTGTCTCTGC TGGAGGATAT
    CTTAAGGCGA TCACCAGCCC AGGGACACCG TTCCACCCGT CTGTTTCCCT ACATGCTGCC
    AACACCTATG GCTTGGGATA CATGAGGTGC TCAACAAATG TTTGTTGGAA GAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40116
    Clone ID Related Accession (Same CDS sequence) XM_014119289.1 , XM_014119291.1 , XM_014119294.1 , XM_014119288.1 , XM_014119290.1 , XM_014119292.1 , XM_014119293.1 , XM_014119295.1 , XM_014119285.1 , XM_014119286.1 , XM_014119287.1
    Accession Version XM_014119288.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product apoptotic protease-activating factor 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGATA TGGAGTATTA TTACTGGAAG AATAGAAAAA
    GACTTTGTCT GTCACCAGGA TACAGTACTT TCTTGTGATA TTTCTCCTGA TGCTACCAAG
    TTTTCATCTA CATCTGCAGA TAAGACTGCG AAGATTTGGA GTTTTGAACT TCTTTCACCT
    CTTCATGAAT TGAGGGGCCA CAAGGGCTGT GTGCGCTGCT CTGTCTTCTC TGTGGACAGT
    ACCCTGTTGG CGACTGGAGA TGACAATGGA GAAATCAGGA TATGGAATGT CTCAAATGGT
    GAGCTGCTTC ATTTGTGTGC TCCGATTTTG GTAGAAGAAG GAGCTGCTAC CCATGGAGGC
    TGGGTGACTG ACCTTTGCTT TTCTCCGGAT AGCAAAATGC TTGTCTCTGC TGGAGGATAT
    CTTAAGGCGA TCACCAGCCC AGGGACACCG TTCCACCCGT CTGTTTCCCT ACATGCTGCC
    AACACCTATG GCTTGGGATA CATGAGGTGC TCAACAAATG TTTGTTGGAA GAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974763.1
    CDS172..3888
    Translation

    Target ORF information:

    RefSeq Version XM_014119288.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014119288.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGATA TGGAGTATTA TTACTGGAAG AATAGAAAAA
    GACTTTGTCT GTCACCAGGA TACAGTACTT TCTTGTGATA TTTCTCCTGA TGCTACCAAG
    TTTTCATCTA CATCTGCAGA TAAGACTGCG AAGATTTGGA GTTTTGAACT TCTTTCACCT
    CTTCATGAAT TGAGGGGCCA CAAGGGCTGT GTGCGCTGCT CTGTCTTCTC TGTGGACAGT
    ACCCTGTTGG CGACTGGAGA TGACAATGGA GAAATCAGGA TATGGAATGT CTCAAATGGT
    GAGCTGCTTC ATTTGTGTGC TCCGATTTTG GTAGAAGAAG GAGCTGCTAC CCATGGAGGC
    TGGGTGACTG ACCTTTGCTT TTCTCCGGAT AGCAAAATGC TTGTCTCTGC TGGAGGATAT
    CTTAAGGCGA TCACCAGCCC AGGGACACCG TTCCACCCGT CTGTTTCCCT ACATGCTGCC
    AACACCTATG GCTTGGGATA CATGAGGTGC TCAACAAATG TTTGTTGGAA GAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40116
    Clone ID Related Accession (Same CDS sequence) XM_014119289.1 , XM_014119291.1 , XM_014119294.1 , XM_014119288.1 , XM_014119290.1 , XM_014119292.1 , XM_014119293.1 , XM_014119295.1 , XM_014119285.1 , XM_014119286.1 , XM_014119287.1
    Accession Version XM_014119290.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product apoptotic protease-activating factor 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGATA TGGAGTATTA TTACTGGAAG AATAGAAAAA
    GACTTTGTCT GTCACCAGGA TACAGTACTT TCTTGTGATA TTTCTCCTGA TGCTACCAAG
    TTTTCATCTA CATCTGCAGA TAAGACTGCG AAGATTTGGA GTTTTGAACT TCTTTCACCT
    CTTCATGAAT TGAGGGGCCA CAAGGGCTGT GTGCGCTGCT CTGTCTTCTC TGTGGACAGT
    ACCCTGTTGG CGACTGGAGA TGACAATGGA GAAATCAGGA TATGGAATGT CTCAAATGGT
    GAGCTGCTTC ATTTGTGTGC TCCGATTTTG GTAGAAGAAG GAGCTGCTAC CCATGGAGGC
    TGGGTGACTG ACCTTTGCTT TTCTCCGGAT AGCAAAATGC TTGTCTCTGC TGGAGGATAT
    CTTAAGGCGA TCACCAGCCC AGGGACACCG TTCCACCCGT CTGTTTCCCT ACATGCTGCC
    AACACCTATG GCTTGGGATA CATGAGGTGC TCAACAAATG TTTGTTGGAA GAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974765.1
    CDS172..3888
    Translation

    Target ORF information:

    RefSeq Version XM_014119290.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014119290.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGATA TGGAGTATTA TTACTGGAAG AATAGAAAAA
    GACTTTGTCT GTCACCAGGA TACAGTACTT TCTTGTGATA TTTCTCCTGA TGCTACCAAG
    TTTTCATCTA CATCTGCAGA TAAGACTGCG AAGATTTGGA GTTTTGAACT TCTTTCACCT
    CTTCATGAAT TGAGGGGCCA CAAGGGCTGT GTGCGCTGCT CTGTCTTCTC TGTGGACAGT
    ACCCTGTTGG CGACTGGAGA TGACAATGGA GAAATCAGGA TATGGAATGT CTCAAATGGT
    GAGCTGCTTC ATTTGTGTGC TCCGATTTTG GTAGAAGAAG GAGCTGCTAC CCATGGAGGC
    TGGGTGACTG ACCTTTGCTT TTCTCCGGAT AGCAAAATGC TTGTCTCTGC TGGAGGATAT
    CTTAAGGCGA TCACCAGCCC AGGGACACCG TTCCACCCGT CTGTTTCCCT ACATGCTGCC
    AACACCTATG GCTTGGGATA CATGAGGTGC TCAACAAATG TTTGTTGGAA GAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40116
    Clone ID Related Accession (Same CDS sequence) XM_014119289.1 , XM_014119291.1 , XM_014119294.1 , XM_014119288.1 , XM_014119290.1 , XM_014119292.1 , XM_014119293.1 , XM_014119295.1 , XM_014119285.1 , XM_014119286.1 , XM_014119287.1
    Accession Version XM_014119292.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product apoptotic protease-activating factor 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGATA TGGAGTATTA TTACTGGAAG AATAGAAAAA
    GACTTTGTCT GTCACCAGGA TACAGTACTT TCTTGTGATA TTTCTCCTGA TGCTACCAAG
    TTTTCATCTA CATCTGCAGA TAAGACTGCG AAGATTTGGA GTTTTGAACT TCTTTCACCT
    CTTCATGAAT TGAGGGGCCA CAAGGGCTGT GTGCGCTGCT CTGTCTTCTC TGTGGACAGT
    ACCCTGTTGG CGACTGGAGA TGACAATGGA GAAATCAGGA TATGGAATGT CTCAAATGGT
    GAGCTGCTTC ATTTGTGTGC TCCGATTTTG GTAGAAGAAG GAGCTGCTAC CCATGGAGGC
    TGGGTGACTG ACCTTTGCTT TTCTCCGGAT AGCAAAATGC TTGTCTCTGC TGGAGGATAT
    CTTAAGGCGA TCACCAGCCC AGGGACACCG TTCCACCCGT CTGTTTCCCT ACATGCTGCC
    AACACCTATG GCTTGGGATA CATGAGGTGC TCAACAAATG TTTGTTGGAA GAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974767.1
    CDS172..3888
    Translation

    Target ORF information:

    RefSeq Version XM_014119292.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014119292.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGATA TGGAGTATTA TTACTGGAAG AATAGAAAAA
    GACTTTGTCT GTCACCAGGA TACAGTACTT TCTTGTGATA TTTCTCCTGA TGCTACCAAG
    TTTTCATCTA CATCTGCAGA TAAGACTGCG AAGATTTGGA GTTTTGAACT TCTTTCACCT
    CTTCATGAAT TGAGGGGCCA CAAGGGCTGT GTGCGCTGCT CTGTCTTCTC TGTGGACAGT
    ACCCTGTTGG CGACTGGAGA TGACAATGGA GAAATCAGGA TATGGAATGT CTCAAATGGT
    GAGCTGCTTC ATTTGTGTGC TCCGATTTTG GTAGAAGAAG GAGCTGCTAC CCATGGAGGC
    TGGGTGACTG ACCTTTGCTT TTCTCCGGAT AGCAAAATGC TTGTCTCTGC TGGAGGATAT
    CTTAAGGCGA TCACCAGCCC AGGGACACCG TTCCACCCGT CTGTTTCCCT ACATGCTGCC
    AACACCTATG GCTTGGGATA CATGAGGTGC TCAACAAATG TTTGTTGGAA GAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40116
    Clone ID Related Accession (Same CDS sequence) XM_014119289.1 , XM_014119291.1 , XM_014119294.1 , XM_014119288.1 , XM_014119290.1 , XM_014119292.1 , XM_014119293.1 , XM_014119295.1 , XM_014119285.1 , XM_014119286.1 , XM_014119287.1
    Accession Version XM_014119293.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product apoptotic protease-activating factor 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGATA TGGAGTATTA TTACTGGAAG AATAGAAAAA
    GACTTTGTCT GTCACCAGGA TACAGTACTT TCTTGTGATA TTTCTCCTGA TGCTACCAAG
    TTTTCATCTA CATCTGCAGA TAAGACTGCG AAGATTTGGA GTTTTGAACT TCTTTCACCT
    CTTCATGAAT TGAGGGGCCA CAAGGGCTGT GTGCGCTGCT CTGTCTTCTC TGTGGACAGT
    ACCCTGTTGG CGACTGGAGA TGACAATGGA GAAATCAGGA TATGGAATGT CTCAAATGGT
    GAGCTGCTTC ATTTGTGTGC TCCGATTTTG GTAGAAGAAG GAGCTGCTAC CCATGGAGGC
    TGGGTGACTG ACCTTTGCTT TTCTCCGGAT AGCAAAATGC TTGTCTCTGC TGGAGGATAT
    CTTAAGGCGA TCACCAGCCC AGGGACACCG TTCCACCCGT CTGTTTCCCT ACATGCTGCC
    AACACCTATG GCTTGGGATA CATGAGGTGC TCAACAAATG TTTGTTGGAA GAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974768.1
    CDS172..3888
    Translation

    Target ORF information:

    RefSeq Version XM_014119293.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014119293.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGATA TGGAGTATTA TTACTGGAAG AATAGAAAAA
    GACTTTGTCT GTCACCAGGA TACAGTACTT TCTTGTGATA TTTCTCCTGA TGCTACCAAG
    TTTTCATCTA CATCTGCAGA TAAGACTGCG AAGATTTGGA GTTTTGAACT TCTTTCACCT
    CTTCATGAAT TGAGGGGCCA CAAGGGCTGT GTGCGCTGCT CTGTCTTCTC TGTGGACAGT
    ACCCTGTTGG CGACTGGAGA TGACAATGGA GAAATCAGGA TATGGAATGT CTCAAATGGT
    GAGCTGCTTC ATTTGTGTGC TCCGATTTTG GTAGAAGAAG GAGCTGCTAC CCATGGAGGC
    TGGGTGACTG ACCTTTGCTT TTCTCCGGAT AGCAAAATGC TTGTCTCTGC TGGAGGATAT
    CTTAAGGCGA TCACCAGCCC AGGGACACCG TTCCACCCGT CTGTTTCCCT ACATGCTGCC
    AACACCTATG GCTTGGGATA CATGAGGTGC TCAACAAATG TTTGTTGGAA GAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40116
    Clone ID Related Accession (Same CDS sequence) XM_014119289.1 , XM_014119291.1 , XM_014119294.1 , XM_014119288.1 , XM_014119290.1 , XM_014119292.1 , XM_014119293.1 , XM_014119295.1 , XM_014119285.1 , XM_014119286.1 , XM_014119287.1
    Accession Version XM_014119295.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product apoptotic protease-activating factor 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGATA TGGAGTATTA TTACTGGAAG AATAGAAAAA
    GACTTTGTCT GTCACCAGGA TACAGTACTT TCTTGTGATA TTTCTCCTGA TGCTACCAAG
    TTTTCATCTA CATCTGCAGA TAAGACTGCG AAGATTTGGA GTTTTGAACT TCTTTCACCT
    CTTCATGAAT TGAGGGGCCA CAAGGGCTGT GTGCGCTGCT CTGTCTTCTC TGTGGACAGT
    ACCCTGTTGG CGACTGGAGA TGACAATGGA GAAATCAGGA TATGGAATGT CTCAAATGGT
    GAGCTGCTTC ATTTGTGTGC TCCGATTTTG GTAGAAGAAG GAGCTGCTAC CCATGGAGGC
    TGGGTGACTG ACCTTTGCTT TTCTCCGGAT AGCAAAATGC TTGTCTCTGC TGGAGGATAT
    CTTAAGGCGA TCACCAGCCC AGGGACACCG TTCCACCCGT CTGTTTCCCT ACATGCTGCC
    AACACCTATG GCTTGGGATA CATGAGGTGC TCAACAAATG TTTGTTGGAA GAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974770.1
    CDS173..3889
    Translation

    Target ORF information:

    RefSeq Version XM_014119295.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014119295.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGATA TGGAGTATTA TTACTGGAAG AATAGAAAAA
    GACTTTGTCT GTCACCAGGA TACAGTACTT TCTTGTGATA TTTCTCCTGA TGCTACCAAG
    TTTTCATCTA CATCTGCAGA TAAGACTGCG AAGATTTGGA GTTTTGAACT TCTTTCACCT
    CTTCATGAAT TGAGGGGCCA CAAGGGCTGT GTGCGCTGCT CTGTCTTCTC TGTGGACAGT
    ACCCTGTTGG CGACTGGAGA TGACAATGGA GAAATCAGGA TATGGAATGT CTCAAATGGT
    GAGCTGCTTC ATTTGTGTGC TCCGATTTTG GTAGAAGAAG GAGCTGCTAC CCATGGAGGC
    TGGGTGACTG ACCTTTGCTT TTCTCCGGAT AGCAAAATGC TTGTCTCTGC TGGAGGATAT
    CTTAAGGCGA TCACCAGCCC AGGGACACCG TTCCACCCGT CTGTTTCCCT ACATGCTGCC
    AACACCTATG GCTTGGGATA CATGAGGTGC TCAACAAATG TTTGTTGGAA GAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40116
    Clone ID Related Accession (Same CDS sequence) XM_014119289.1 , XM_014119291.1 , XM_014119294.1 , XM_014119288.1 , XM_014119290.1 , XM_014119292.1 , XM_014119293.1 , XM_014119295.1 , XM_014119285.1 , XM_014119286.1 , XM_014119287.1
    Accession Version XM_014119285.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product apoptotic protease-activating factor 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGATA TGGAGTATTA TTACTGGAAG AATAGAAAAA
    GACTTTGTCT GTCACCAGGA TACAGTACTT TCTTGTGATA TTTCTCCTGA TGCTACCAAG
    TTTTCATCTA CATCTGCAGA TAAGACTGCG AAGATTTGGA GTTTTGAACT TCTTTCACCT
    CTTCATGAAT TGAGGGGCCA CAAGGGCTGT GTGCGCTGCT CTGTCTTCTC TGTGGACAGT
    ACCCTGTTGG CGACTGGAGA TGACAATGGA GAAATCAGGA TATGGAATGT CTCAAATGGT
    GAGCTGCTTC ATTTGTGTGC TCCGATTTTG GTAGAAGAAG GAGCTGCTAC CCATGGAGGC
    TGGGTGACTG ACCTTTGCTT TTCTCCGGAT AGCAAAATGC TTGTCTCTGC TGGAGGATAT
    CTTAAGGCGA TCACCAGCCC AGGGACACCG TTCCACCCGT CTGTTTCCCT ACATGCTGCC
    AACACCTATG GCTTGGGATA CATGAGGTGC TCAACAAATG TTTGTTGGAA GAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974760.1
    CDS172..3888
    Translation

    Target ORF information:

    RefSeq Version XM_014119285.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014119285.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGATA TGGAGTATTA TTACTGGAAG AATAGAAAAA
    GACTTTGTCT GTCACCAGGA TACAGTACTT TCTTGTGATA TTTCTCCTGA TGCTACCAAG
    TTTTCATCTA CATCTGCAGA TAAGACTGCG AAGATTTGGA GTTTTGAACT TCTTTCACCT
    CTTCATGAAT TGAGGGGCCA CAAGGGCTGT GTGCGCTGCT CTGTCTTCTC TGTGGACAGT
    ACCCTGTTGG CGACTGGAGA TGACAATGGA GAAATCAGGA TATGGAATGT CTCAAATGGT
    GAGCTGCTTC ATTTGTGTGC TCCGATTTTG GTAGAAGAAG GAGCTGCTAC CCATGGAGGC
    TGGGTGACTG ACCTTTGCTT TTCTCCGGAT AGCAAAATGC TTGTCTCTGC TGGAGGATAT
    CTTAAGGCGA TCACCAGCCC AGGGACACCG TTCCACCCGT CTGTTTCCCT ACATGCTGCC
    AACACCTATG GCTTGGGATA CATGAGGTGC TCAACAAATG TTTGTTGGAA GAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40116
    Clone ID Related Accession (Same CDS sequence) XM_014119289.1 , XM_014119291.1 , XM_014119294.1 , XM_014119288.1 , XM_014119290.1 , XM_014119292.1 , XM_014119293.1 , XM_014119295.1 , XM_014119285.1 , XM_014119286.1 , XM_014119287.1
    Accession Version XM_014119286.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product apoptotic protease-activating factor 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGATA TGGAGTATTA TTACTGGAAG AATAGAAAAA
    GACTTTGTCT GTCACCAGGA TACAGTACTT TCTTGTGATA TTTCTCCTGA TGCTACCAAG
    TTTTCATCTA CATCTGCAGA TAAGACTGCG AAGATTTGGA GTTTTGAACT TCTTTCACCT
    CTTCATGAAT TGAGGGGCCA CAAGGGCTGT GTGCGCTGCT CTGTCTTCTC TGTGGACAGT
    ACCCTGTTGG CGACTGGAGA TGACAATGGA GAAATCAGGA TATGGAATGT CTCAAATGGT
    GAGCTGCTTC ATTTGTGTGC TCCGATTTTG GTAGAAGAAG GAGCTGCTAC CCATGGAGGC
    TGGGTGACTG ACCTTTGCTT TTCTCCGGAT AGCAAAATGC TTGTCTCTGC TGGAGGATAT
    CTTAAGGCGA TCACCAGCCC AGGGACACCG TTCCACCCGT CTGTTTCCCT ACATGCTGCC
    AACACCTATG GCTTGGGATA CATGAGGTGC TCAACAAATG TTTGTTGGAA GAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974761.1
    CDS172..3888
    Translation

    Target ORF information:

    RefSeq Version XM_014119286.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014119286.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGATA TGGAGTATTA TTACTGGAAG AATAGAAAAA
    GACTTTGTCT GTCACCAGGA TACAGTACTT TCTTGTGATA TTTCTCCTGA TGCTACCAAG
    TTTTCATCTA CATCTGCAGA TAAGACTGCG AAGATTTGGA GTTTTGAACT TCTTTCACCT
    CTTCATGAAT TGAGGGGCCA CAAGGGCTGT GTGCGCTGCT CTGTCTTCTC TGTGGACAGT
    ACCCTGTTGG CGACTGGAGA TGACAATGGA GAAATCAGGA TATGGAATGT CTCAAATGGT
    GAGCTGCTTC ATTTGTGTGC TCCGATTTTG GTAGAAGAAG GAGCTGCTAC CCATGGAGGC
    TGGGTGACTG ACCTTTGCTT TTCTCCGGAT AGCAAAATGC TTGTCTCTGC TGGAGGATAT
    CTTAAGGCGA TCACCAGCCC AGGGACACCG TTCCACCCGT CTGTTTCCCT ACATGCTGCC
    AACACCTATG GCTTGGGATA CATGAGGTGC TCAACAAATG TTTGTTGGAA GAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40116
    Clone ID Related Accession (Same CDS sequence) XM_014119289.1 , XM_014119291.1 , XM_014119294.1 , XM_014119288.1 , XM_014119290.1 , XM_014119292.1 , XM_014119293.1 , XM_014119295.1 , XM_014119285.1 , XM_014119286.1 , XM_014119287.1
    Accession Version XM_014119287.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product apoptotic protease-activating factor 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGATA TGGAGTATTA TTACTGGAAG AATAGAAAAA
    GACTTTGTCT GTCACCAGGA TACAGTACTT TCTTGTGATA TTTCTCCTGA TGCTACCAAG
    TTTTCATCTA CATCTGCAGA TAAGACTGCG AAGATTTGGA GTTTTGAACT TCTTTCACCT
    CTTCATGAAT TGAGGGGCCA CAAGGGCTGT GTGCGCTGCT CTGTCTTCTC TGTGGACAGT
    ACCCTGTTGG CGACTGGAGA TGACAATGGA GAAATCAGGA TATGGAATGT CTCAAATGGT
    GAGCTGCTTC ATTTGTGTGC TCCGATTTTG GTAGAAGAAG GAGCTGCTAC CCATGGAGGC
    TGGGTGACTG ACCTTTGCTT TTCTCCGGAT AGCAAAATGC TTGTCTCTGC TGGAGGATAT
    CTTAAGGCGA TCACCAGCCC AGGGACACCG TTCCACCCGT CTGTTTCCCT ACATGCTGCC
    AACACCTATG GCTTGGGATA CATGAGGTGC TCAACAAATG TTTGTTGGAA GAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974762.1
    CDS172..3888
    Translation

    Target ORF information:

    RefSeq Version XM_014119287.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014119287.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGATA TGGAGTATTA TTACTGGAAG AATAGAAAAA
    GACTTTGTCT GTCACCAGGA TACAGTACTT TCTTGTGATA TTTCTCCTGA TGCTACCAAG
    TTTTCATCTA CATCTGCAGA TAAGACTGCG AAGATTTGGA GTTTTGAACT TCTTTCACCT
    CTTCATGAAT TGAGGGGCCA CAAGGGCTGT GTGCGCTGCT CTGTCTTCTC TGTGGACAGT
    ACCCTGTTGG CGACTGGAGA TGACAATGGA GAAATCAGGA TATGGAATGT CTCAAATGGT
    GAGCTGCTTC ATTTGTGTGC TCCGATTTTG GTAGAAGAAG GAGCTGCTAC CCATGGAGGC
    TGGGTGACTG ACCTTTGCTT TTCTCCGGAT AGCAAAATGC TTGTCTCTGC TGGAGGATAT
    CTTAAGGCGA TCACCAGCCC AGGGACACCG TTCCACCCGT CTGTTTCCCT ACATGCTGCC
    AACACCTATG GCTTGGGATA CATGAGGTGC TCAACAAATG TTTGTTGGAA GAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40117
    Clone ID Related Accession (Same CDS sequence) XM_014119296.1
    Accession Version XM_014119296.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3654bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product apoptotic protease-activating factor 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGATA TGGAGTATTA TTACTGGAAG AATAGAAAAA
    GACTTTGTCT GTCACCAGGA TACAGTACTT TCTTGTGATA TTTCTCCTGA TGCTACCAAG
    TTTTCATCTA CATCTGCAGA TAAGACTGCG AAGATTTGGA GTTTTGAACT TCTTTCACCT
    CTTCATGAAT TGAGGGGCCA CAAGGGCTGT GTGCGCTGCT CTGTCTTCTC TGTGGACAGT
    ACCCTGTTGG CGACTGGAGA TGACAATGGA GAAATCAGGA TATGGAATGT CTCAAATGGT
    GAGCTGCTTC ATTTGTGTGC TCCGATTTTG GTAGAAGAAG GAGCTGCTAC CCATGGAGGC
    TGGGTGACTG ACCTTTGCTT TTCTCCGGAT AGCAAAATGC TTGTCTCTGC TGGAGGATAT
    CTTAAGGGCT TGGGATACAT GAGGTGCTCA ACAAATGTTT GTTGGAAGAA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974771.1
    CDS172..3825
    Translation

    Target ORF information:

    RefSeq Version XM_014119296.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014119296.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGATA TGGAGTATTA TTACTGGAAG AATAGAAAAA
    GACTTTGTCT GTCACCAGGA TACAGTACTT TCTTGTGATA TTTCTCCTGA TGCTACCAAG
    TTTTCATCTA CATCTGCAGA TAAGACTGCG AAGATTTGGA GTTTTGAACT TCTTTCACCT
    CTTCATGAAT TGAGGGGCCA CAAGGGCTGT GTGCGCTGCT CTGTCTTCTC TGTGGACAGT
    ACCCTGTTGG CGACTGGAGA TGACAATGGA GAAATCAGGA TATGGAATGT CTCAAATGGT
    GAGCTGCTTC ATTTGTGTGC TCCGATTTTG GTAGAAGAAG GAGCTGCTAC CCATGGAGGC
    TGGGTGACTG ACCTTTGCTT TTCTCCGGAT AGCAAAATGC TTGTCTCTGC TGGAGGATAT
    CTTAAGGGCT TGGGATACAT GAGGTGCTCA ACAAATGTTT GTTGGAAGAA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40118
    Clone ID Related Accession (Same CDS sequence) XM_014119299.1 , XM_014119299.2
    Accession Version XM_014119299.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3234bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product apoptotic protease-activating factor 1 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGTCA TCTACATCTG CTGTGTATTT ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974774.1
    CDS173..3406
    Translation

    Target ORF information:

    RefSeq Version XM_014119299.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014119299.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGTCA TCTACATCTG CTGTGTATTT ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40118
    Clone ID Related Accession (Same CDS sequence) XM_014119299.1 , XM_014119299.2
    Accession Version XM_014119299.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3234bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product apoptotic protease-activating factor 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014119299.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGTCA TCTACATCTG CTGTGTATTT ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974774.1
    CDS137..3370
    Translation

    Target ORF information:

    RefSeq Version XM_014119299.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014119299.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGTCA TCTACATCTG CTGTGTATTT ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40120
    Clone ID Related Accession (Same CDS sequence) XM_014119301.1
    Accession Version XM_014119301.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3063bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product apoptotic protease-activating factor 1 isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCTCAGGA AGTACTTCTT AAGGTCTCTG TTGATCTTGG ATGATATTTG GGATTCCTGG 
    ATATTAAAAG CTTTTGACAA TCAGTGTCAG ATTCTTCTTA CAACCAGAGA CAAGAGCGTT
    ACAGATTCAG TAATGGGTCC TAAATATATA GTCGCTGTGG AGAGTGACTT AGGAAAAGAA
    AAAGGACTTG AAATTCTGTC CCTCTTTGTT AATATGAAGA AAGCAGATTT GCCAGAGCAA
    GCTCATAGTA TTATAAAAGA ATGTAAAGGT TCTCCTCTCG TAGTGTCTTT AATTGGTGCA
    CTTTTACGTG ATTTTCCTAA CCGTTGGGAT TACTACCTCA GGCAACTTCA GAATAAGCAA
    TTTAAGAGAA TAAGGAAATC TTCATTTTAT GATTATGAGG CTCTGGATGA AGCCATGTCT
    ATAAGTGTTG AAATGCTCAG AGAGGACATC AAAGATTACT ACACAGATCT TTCCATCCTT
    CAGAAGGATG TTAAGGTGCC TACAAAGGTG TTATGTATTC TCTGGGACAT GGAAACTGAA
    GAAGTTGAAG ATATTCTGCA GGAGTTTGTT AATAAGTCTC TCTTATTCTG TGATCGGAAT
    GGAAAATCAT TTCGTTATTA TTTACATGAT CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT
    CGCAATCAAC TTCAGGATCT ACATAAGAAG ATAGTCACTC AGTTCCAGAA ACATTACCAG
    CCACATACTC TTTCACCAGA TCAGGAGGAC TGCATGTACT GGTACAATTT TCTGGCCTAC
    CACATGGCTA GTGCCAGTAT GCACAAAGAA CTTTGTGCTT TAATGTTTTC CTTGGATTGG
    ATTAAAGCAA AAACTGAACT CGTAGGCCCT GCTCATCTGA TTCATGAATT TGTGGAATAC
    AGGCATATAT TGGATGAAAA GGATTGTGCA GTCTGTGAAA ATTTCCAGGA ATTTTTATCT
    TTAAATGGAC ATCTTCTTGG ACGACAGCCA TTTCCTAATA TTGTACAACT GGGTCTCTGT
    GAACCAGAAA CTTCAGAAGT TTATCAGCAA GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT
    CACGGAATGC TTTACCTGGA GTGGATAAAC AAAAAAAACA TCAAGAATCT TTCCCGCTTA
    GTTGTCCGCC CCCATACAGA TGCTGTTTAC CATGCTTGCT TTTCTGAGGA TGGTCAAAGA
    ATAGCTTCTT GTGGAGTTGA TAAAACCTTA CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA
    CTGCTAGAAA TCAAGGCTCA TGAAGATGAA GTGCTTTGTT GTGCATTCTC TACAGATGAC
    AGATTTATAG CAACCTGCTC AGTGGATAAA AAAGTGAAGA TTTGGAATTC TATGACAGGG
    GGACTAGTAC ATGTCTATGA TGAGCACTCA GAGCAAGTCA ACTGCTGCCA CTTTACCAAC
    AACAGTCATT ACCTTCTCTT AGCCACTGCA TCAAGTGACT GCTTCCTCAA ACTCTGGGAT
    TTGAATCAAA AAGAATGTCG AAATACCATG TTTGGCCATA CAAATTCAGT CAATCACTGC
    AGATTTTCAC CAGATGATAA GCTTTTGGCT AGTTGTTCAG CTGATGGAAC ATTAAAGCTT
    TGGGATGTAA AATCAGCAAA TGAGAGGAAA AGCATTAATG TGAAGCAGTT CTTCCTAAAT
    TCAGAGGAGC CTCAAGAGGA TATGGAAGTG ATAGTGAAAT GTTGTTCATG GTCTGCTGAT
    GGCACTAGGA TAATGGTGGC AGCAAAAAAT AAAATCTTTC TATTTGACAT TCATACTGGT
    GGCTTATTGG CAGAAATCCA TACAGGCCAT CACAGCACCA TCCAGTACTG TGACTTCTCC
    CCCCAAAATC ATTTGGCAGT AGTGGCTTTG TCCCAGTGCT GTGTGGAGTT GTGGAACATG
    GACTCCTGTT TAAAAGTGGC TGATTGCAGA GGACATTTAA GTTGGGTTCA TTGTGTGATG
    TTTTCTCCCG ATGGATCATC ATTTTTGACA TCTTCTGATG ACCAGACAAT CAGGCTCTGG
    GAGACCAAAA AAGTATGTAA GAACTCTGCT ATAGTGTTAA AGCAAGAAAT AGATGTTGTG
    TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA GTTGACAATA TAAAACGTCT GCAGCTCATT
    AATGGAAAAA CAGGTCAGAT TGATTATCTG ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA
    AGTCCACATC TTCAGTACAT TGCATTTGGA GGTGAAGATG GAGCCGTTGA GATTTTAGAA
    CTCCTAAACA ACAGAATCTT CCAATCCAGG ATTGGGCACA AGAAAACTGT GCGGCACATC
    CAGTTCACAG ATGATGGGAA GACTCTTATT TCAAGCTCTG ATGATTCTTC AATTCAGGTA
    TGGAATTGGC AGTCAGAGGA ATATGTCTTC CTACAAGCCC ATCAGGAAAC TGTGAAAGAC
    TTTAAGCTCC TGAAAAATTC AAAACTGCTT TCCTGGTCAT TTGATGGAAC AGTAAAGATA
    TGGAGTATTA TTACTGGAAG AATAGAAAAA GACTTTGTCT GTCACCAGGA TACAGTACTT
    TCTTGTGATA TTTCTCCTGA TGCTACCAAG TTTTCATCTA CATCTGCAGA TAAGACTGCG
    AAGATTTGGA GTTTTGAACT TCTTTCACCT CTTCATGAAT TGAGGGGCCA CAAGGGCTGT
    GTGCGCTGCT CTGTCTTCTC TGTGGACAGT ACCCTGTTGG CGACTGGAGA TGACAATGGA
    GAAATCAGGA TATGGAATGT CTCAAATGGT GAGCTGCTTC ATTTGTGTGC TCCGATTTTG
    GTAGAAGAAG GAGCTGCTAC CCATGGAGGC TGGGTGACTG ACCTTTGCTT TTCTCCGGAT
    AGCAAAATGC TTGTCTCTGC TGGAGGATAT CTTAAGTGGT GGAATGTTGT CACTGGGGAA
    TCCTCACAAA CCTTCTACAC AAATGGAACC TGTCTTAAGA AAATACACGT TTCCTCTGAC
    TTCAAAACAT ATGTGACTGT TGATAATCTT GGGATTTTAT ATATTTTACA GATGTTAGAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974776.1
    CDS72..3134
    Translation

    Target ORF information:

    RefSeq Version XM_014119301.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014119301.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCTCAGGA AGTACTTCTT AAGGTCTCTG TTGATCTTGG ATGATATTTG GGATTCCTGG 
    ATATTAAAAG CTTTTGACAA TCAGTGTCAG ATTCTTCTTA CAACCAGAGA CAAGAGCGTT
    ACAGATTCAG TAATGGGTCC TAAATATATA GTCGCTGTGG AGAGTGACTT AGGAAAAGAA
    AAAGGACTTG AAATTCTGTC CCTCTTTGTT AATATGAAGA AAGCAGATTT GCCAGAGCAA
    GCTCATAGTA TTATAAAAGA ATGTAAAGGT TCTCCTCTCG TAGTGTCTTT AATTGGTGCA
    CTTTTACGTG ATTTTCCTAA CCGTTGGGAT TACTACCTCA GGCAACTTCA GAATAAGCAA
    TTTAAGAGAA TAAGGAAATC TTCATTTTAT GATTATGAGG CTCTGGATGA AGCCATGTCT
    ATAAGTGTTG AAATGCTCAG AGAGGACATC AAAGATTACT ACACAGATCT TTCCATCCTT
    CAGAAGGATG TTAAGGTGCC TACAAAGGTG TTATGTATTC TCTGGGACAT GGAAACTGAA
    GAAGTTGAAG ATATTCTGCA GGAGTTTGTT AATAAGTCTC TCTTATTCTG TGATCGGAAT
    GGAAAATCAT TTCGTTATTA TTTACATGAT CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT
    CGCAATCAAC TTCAGGATCT ACATAAGAAG ATAGTCACTC AGTTCCAGAA ACATTACCAG
    CCACATACTC TTTCACCAGA TCAGGAGGAC TGCATGTACT GGTACAATTT TCTGGCCTAC
    CACATGGCTA GTGCCAGTAT GCACAAAGAA CTTTGTGCTT TAATGTTTTC CTTGGATTGG
    ATTAAAGCAA AAACTGAACT CGTAGGCCCT GCTCATCTGA TTCATGAATT TGTGGAATAC
    AGGCATATAT TGGATGAAAA GGATTGTGCA GTCTGTGAAA ATTTCCAGGA ATTTTTATCT
    TTAAATGGAC ATCTTCTTGG ACGACAGCCA TTTCCTAATA TTGTACAACT GGGTCTCTGT
    GAACCAGAAA CTTCAGAAGT TTATCAGCAA GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT
    CACGGAATGC TTTACCTGGA GTGGATAAAC AAAAAAAACA TCAAGAATCT TTCCCGCTTA
    GTTGTCCGCC CCCATACAGA TGCTGTTTAC CATGCTTGCT TTTCTGAGGA TGGTCAAAGA
    ATAGCTTCTT GTGGAGTTGA TAAAACCTTA CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA
    CTGCTAGAAA TCAAGGCTCA TGAAGATGAA GTGCTTTGTT GTGCATTCTC TACAGATGAC
    AGATTTATAG CAACCTGCTC AGTGGATAAA AAAGTGAAGA TTTGGAATTC TATGACAGGG
    GGACTAGTAC ATGTCTATGA TGAGCACTCA GAGCAAGTCA ACTGCTGCCA CTTTACCAAC
    AACAGTCATT ACCTTCTCTT AGCCACTGCA TCAAGTGACT GCTTCCTCAA ACTCTGGGAT
    TTGAATCAAA AAGAATGTCG AAATACCATG TTTGGCCATA CAAATTCAGT CAATCACTGC
    AGATTTTCAC CAGATGATAA GCTTTTGGCT AGTTGTTCAG CTGATGGAAC ATTAAAGCTT
    TGGGATGTAA AATCAGCAAA TGAGAGGAAA AGCATTAATG TGAAGCAGTT CTTCCTAAAT
    TCAGAGGAGC CTCAAGAGGA TATGGAAGTG ATAGTGAAAT GTTGTTCATG GTCTGCTGAT
    GGCACTAGGA TAATGGTGGC AGCAAAAAAT AAAATCTTTC TATTTGACAT TCATACTGGT
    GGCTTATTGG CAGAAATCCA TACAGGCCAT CACAGCACCA TCCAGTACTG TGACTTCTCC
    CCCCAAAATC ATTTGGCAGT AGTGGCTTTG TCCCAGTGCT GTGTGGAGTT GTGGAACATG
    GACTCCTGTT TAAAAGTGGC TGATTGCAGA GGACATTTAA GTTGGGTTCA TTGTGTGATG
    TTTTCTCCCG ATGGATCATC ATTTTTGACA TCTTCTGATG ACCAGACAAT CAGGCTCTGG
    GAGACCAAAA AAGTATGTAA GAACTCTGCT ATAGTGTTAA AGCAAGAAAT AGATGTTGTG
    TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA GTTGACAATA TAAAACGTCT GCAGCTCATT
    AATGGAAAAA CAGGTCAGAT TGATTATCTG ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA
    AGTCCACATC TTCAGTACAT TGCATTTGGA GGTGAAGATG GAGCCGTTGA GATTTTAGAA
    CTCCTAAACA ACAGAATCTT CCAATCCAGG ATTGGGCACA AGAAAACTGT GCGGCACATC
    CAGTTCACAG ATGATGGGAA GACTCTTATT TCAAGCTCTG ATGATTCTTC AATTCAGGTA
    TGGAATTGGC AGTCAGAGGA ATATGTCTTC CTACAAGCCC ATCAGGAAAC TGTGAAAGAC
    TTTAAGCTCC TGAAAAATTC AAAACTGCTT TCCTGGTCAT TTGATGGAAC AGTAAAGATA
    TGGAGTATTA TTACTGGAAG AATAGAAAAA GACTTTGTCT GTCACCAGGA TACAGTACTT
    TCTTGTGATA TTTCTCCTGA TGCTACCAAG TTTTCATCTA CATCTGCAGA TAAGACTGCG
    AAGATTTGGA GTTTTGAACT TCTTTCACCT CTTCATGAAT TGAGGGGCCA CAAGGGCTGT
    GTGCGCTGCT CTGTCTTCTC TGTGGACAGT ACCCTGTTGG CGACTGGAGA TGACAATGGA
    GAAATCAGGA TATGGAATGT CTCAAATGGT GAGCTGCTTC ATTTGTGTGC TCCGATTTTG
    GTAGAAGAAG GAGCTGCTAC CCATGGAGGC TGGGTGACTG ACCTTTGCTT TTCTCCGGAT
    AGCAAAATGC TTGTCTCTGC TGGAGGATAT CTTAAGTGGT GGAATGTTGT CACTGGGGAA
    TCCTCACAAA CCTTCTACAC AAATGGAACC TGTCTTAAGA AAATACACGT TTCCTCTGAC
    TTCAAAACAT ATGTGACTGT TGATAATCTT GGGATTTTAT ATATTTTACA GATGTTAGAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe17604
    Clone ID Related Accession (Same CDS sequence) XM_014119284.1 , XM_005629260.2 , XM_003432034.4 , XM_003432034.3
    Accession Version XM_014119284.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3753bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product apoptotic protease-activating factor 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGATA TGGAGTATTA TTACTGGAAG AATAGAAAAA
    GACTTTGTCT GTCACCAGGA TACAGTACTT TCTTGTGATA TTTCTCCTGA TGCTACCAAG
    TTTTCATCTA CATCTGCAGA TAAGACTGCG AAGATTTGGA GTTTTGAACT TCTTTCACCT
    CTTCATGAAT TGAGGGGCCA CAAGGGCTGT GTGCGCTGCT CTGTCTTCTC TGTGGACAGT
    ACCCTGTTGG CGACTGGAGA TGACAATGGA GAAATCAGGA TATGGAATGT CTCAAATGGT
    GAGCTGCTTC ATTTGTGTGC TCCGATTTTG GTAGAAGAAG GAGCTGCTAC CCATGGAGGC
    TGGGTGACTG ACCTTTGCTT TTCTCCGGAT AGCAAAATGC TTGTCTCTGC TGGAGGATAT
    CTTAAGTGGT GGAATGTTGT CACTGGGGAA TCCTCACAAA CCTTCTACAC AAATGGAACC
    TGTCTTAAGA AAATACACGT TTCCTCTGAC TTCAAAACAT ATGTGACTGT TGATAATCTT
    GGGATTTTAT ATATTTTACA GATGTTAGAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974759.1
    CDS551..4303
    Translation

    Target ORF information:

    RefSeq Version XM_014119284.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014119284.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGATA TGGAGTATTA TTACTGGAAG AATAGAAAAA
    GACTTTGTCT GTCACCAGGA TACAGTACTT TCTTGTGATA TTTCTCCTGA TGCTACCAAG
    TTTTCATCTA CATCTGCAGA TAAGACTGCG AAGATTTGGA GTTTTGAACT TCTTTCACCT
    CTTCATGAAT TGAGGGGCCA CAAGGGCTGT GTGCGCTGCT CTGTCTTCTC TGTGGACAGT
    ACCCTGTTGG CGACTGGAGA TGACAATGGA GAAATCAGGA TATGGAATGT CTCAAATGGT
    GAGCTGCTTC ATTTGTGTGC TCCGATTTTG GTAGAAGAAG GAGCTGCTAC CCATGGAGGC
    TGGGTGACTG ACCTTTGCTT TTCTCCGGAT AGCAAAATGC TTGTCTCTGC TGGAGGATAT
    CTTAAGTGGT GGAATGTTGT CACTGGGGAA TCCTCACAAA CCTTCTACAC AAATGGAACC
    TGTCTTAAGA AAATACACGT TTCCTCTGAC TTCAAAACAT ATGTGACTGT TGATAATCTT
    GGGATTTTAT ATATTTTACA GATGTTAGAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe17604
    Clone ID Related Accession (Same CDS sequence) XM_014119284.1 , XM_005629260.2 , XM_003432034.4 , XM_003432034.3
    Accession Version XM_005629260.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3753bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product apoptotic protease-activating factor 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005629260.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGATA TGGAGTATTA TTACTGGAAG AATAGAAAAA
    GACTTTGTCT GTCACCAGGA TACAGTACTT TCTTGTGATA TTTCTCCTGA TGCTACCAAG
    TTTTCATCTA CATCTGCAGA TAAGACTGCG AAGATTTGGA GTTTTGAACT TCTTTCACCT
    CTTCATGAAT TGAGGGGCCA CAAGGGCTGT GTGCGCTGCT CTGTCTTCTC TGTGGACAGT
    ACCCTGTTGG CGACTGGAGA TGACAATGGA GAAATCAGGA TATGGAATGT CTCAAATGGT
    GAGCTGCTTC ATTTGTGTGC TCCGATTTTG GTAGAAGAAG GAGCTGCTAC CCATGGAGGC
    TGGGTGACTG ACCTTTGCTT TTCTCCGGAT AGCAAAATGC TTGTCTCTGC TGGAGGATAT
    CTTAAGTGGT GGAATGTTGT CACTGGGGAA TCCTCACAAA CCTTCTACAC AAATGGAACC
    TGTCTTAAGA AAATACACGT TTCCTCTGAC TTCAAAACAT ATGTGACTGT TGATAATCTT
    GGGATTTTAT ATATTTTACA GATGTTAGAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005629317.1
    CDS159..3911
    Translation

    Target ORF information:

    RefSeq Version XM_005629260.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005629260.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGATA TGGAGTATTA TTACTGGAAG AATAGAAAAA
    GACTTTGTCT GTCACCAGGA TACAGTACTT TCTTGTGATA TTTCTCCTGA TGCTACCAAG
    TTTTCATCTA CATCTGCAGA TAAGACTGCG AAGATTTGGA GTTTTGAACT TCTTTCACCT
    CTTCATGAAT TGAGGGGCCA CAAGGGCTGT GTGCGCTGCT CTGTCTTCTC TGTGGACAGT
    ACCCTGTTGG CGACTGGAGA TGACAATGGA GAAATCAGGA TATGGAATGT CTCAAATGGT
    GAGCTGCTTC ATTTGTGTGC TCCGATTTTG GTAGAAGAAG GAGCTGCTAC CCATGGAGGC
    TGGGTGACTG ACCTTTGCTT TTCTCCGGAT AGCAAAATGC TTGTCTCTGC TGGAGGATAT
    CTTAAGTGGT GGAATGTTGT CACTGGGGAA TCCTCACAAA CCTTCTACAC AAATGGAACC
    TGTCTTAAGA AAATACACGT TTCCTCTGAC TTCAAAACAT ATGTGACTGT TGATAATCTT
    GGGATTTTAT ATATTTTACA GATGTTAGAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe17604
    Clone ID Related Accession (Same CDS sequence) XM_014119284.1 , XM_005629260.2 , XM_003432034.4 , XM_003432034.3
    Accession Version XM_003432034.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3753bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product apoptotic protease-activating factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003432034.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGATA TGGAGTATTA TTACTGGAAG AATAGAAAAA
    GACTTTGTCT GTCACCAGGA TACAGTACTT TCTTGTGATA TTTCTCCTGA TGCTACCAAG
    TTTTCATCTA CATCTGCAGA TAAGACTGCG AAGATTTGGA GTTTTGAACT TCTTTCACCT
    CTTCATGAAT TGAGGGGCCA CAAGGGCTGT GTGCGCTGCT CTGTCTTCTC TGTGGACAGT
    ACCCTGTTGG CGACTGGAGA TGACAATGGA GAAATCAGGA TATGGAATGT CTCAAATGGT
    GAGCTGCTTC ATTTGTGTGC TCCGATTTTG GTAGAAGAAG GAGCTGCTAC CCATGGAGGC
    TGGGTGACTG ACCTTTGCTT TTCTCCGGAT AGCAAAATGC TTGTCTCTGC TGGAGGATAT
    CTTAAGTGGT GGAATGTTGT CACTGGGGAA TCCTCACAAA CCTTCTACAC AAATGGAACC
    TGTCTTAAGA AAATACACGT TTCCTCTGAC TTCAAAACAT ATGTGACTGT TGATAATCTT
    GGGATTTTAT ATATTTTACA GATGTTAGAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003432082.1
    CDS137..3889
    Translation

    Target ORF information:

    RefSeq Version XM_003432034.4
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003432034.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGATA TGGAGTATTA TTACTGGAAG AATAGAAAAA
    GACTTTGTCT GTCACCAGGA TACAGTACTT TCTTGTGATA TTTCTCCTGA TGCTACCAAG
    TTTTCATCTA CATCTGCAGA TAAGACTGCG AAGATTTGGA GTTTTGAACT TCTTTCACCT
    CTTCATGAAT TGAGGGGCCA CAAGGGCTGT GTGCGCTGCT CTGTCTTCTC TGTGGACAGT
    ACCCTGTTGG CGACTGGAGA TGACAATGGA GAAATCAGGA TATGGAATGT CTCAAATGGT
    GAGCTGCTTC ATTTGTGTGC TCCGATTTTG GTAGAAGAAG GAGCTGCTAC CCATGGAGGC
    TGGGTGACTG ACCTTTGCTT TTCTCCGGAT AGCAAAATGC TTGTCTCTGC TGGAGGATAT
    CTTAAGTGGT GGAATGTTGT CACTGGGGAA TCCTCACAAA CCTTCTACAC AAATGGAACC
    TGTCTTAAGA AAATACACGT TTCCTCTGAC TTCAAAACAT ATGTGACTGT TGATAATCTT
    GGGATTTTAT ATATTTTACA GATGTTAGAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe17604
    Clone ID Related Accession (Same CDS sequence) XM_014119284.1 , XM_005629260.2 , XM_003432034.4 , XM_003432034.3
    Accession Version XM_003432034.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3753bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product apoptotic protease-activating factor 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003432034.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGATA TGGAGTATTA TTACTGGAAG AATAGAAAAA
    GACTTTGTCT GTCACCAGGA TACAGTACTT TCTTGTGATA TTTCTCCTGA TGCTACCAAG
    TTTTCATCTA CATCTGCAGA TAAGACTGCG AAGATTTGGA GTTTTGAACT TCTTTCACCT
    CTTCATGAAT TGAGGGGCCA CAAGGGCTGT GTGCGCTGCT CTGTCTTCTC TGTGGACAGT
    ACCCTGTTGG CGACTGGAGA TGACAATGGA GAAATCAGGA TATGGAATGT CTCAAATGGT
    GAGCTGCTTC ATTTGTGTGC TCCGATTTTG GTAGAAGAAG GAGCTGCTAC CCATGGAGGC
    TGGGTGACTG ACCTTTGCTT TTCTCCGGAT AGCAAAATGC TTGTCTCTGC TGGAGGATAT
    CTTAAGTGGT GGAATGTTGT CACTGGGGAA TCCTCACAAA CCTTCTACAC AAATGGAACC
    TGTCTTAAGA AAATACACGT TTCCTCTGAC TTCAAAACAT ATGTGACTGT TGATAATCTT
    GGGATTTTAT ATATTTTACA GATGTTAGAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003432082.1
    CDS171..3923
    Translation

    Target ORF information:

    RefSeq Version XM_003432034.3
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003432034.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTT TGGGATGTAA AATCAGCAAA TGAGAGGAAA
    AGCATTAATG TGAAGCAGTT CTTCCTAAAT TCAGAGGAGC CTCAAGAGGA TATGGAAGTG
    ATAGTGAAAT GTTGTTCATG GTCTGCTGAT GGCACTAGGA TAATGGTGGC AGCAAAAAAT
    AAAATCTTTC TATTTGACAT TCATACTGGT GGCTTATTGG CAGAAATCCA TACAGGCCAT
    CACAGCACCA TCCAGTACTG TGACTTCTCC CCCCAAAATC ATTTGGCAGT AGTGGCTTTG
    TCCCAGTGCT GTGTGGAGTT GTGGAACATG GACTCCTGTT TAAAAGTGGC TGATTGCAGA
    GGACATTTAA GTTGGGTTCA TTGTGTGATG TTTTCTCCCG ATGGATCATC ATTTTTGACA
    TCTTCTGATG ACCAGACAAT CAGGCTCTGG GAGACCAAAA AAGTATGTAA GAACTCTGCT
    ATAGTGTTAA AGCAAGAAAT AGATGTTGTG TTTCAAGAAA ATGAAGTGAT GGTTCTTGCA
    GTTGACAATA TAAAACGTCT GCAGCTCATT AATGGAAAAA CAGGTCAGAT TGATTATCTG
    ATTGAAGCTC AGGTTAGTTG CTGTTGCCTA AGTCCACATC TTCAGTACAT TGCATTTGGA
    GGTGAAGATG GAGCCGTTGA GATTTTAGAA CTCCTAAACA ACAGAATCTT CCAATCCAGG
    ATTGGGCACA AGAAAACTGT GCGGCACATC CAGTTCACAG ATGATGGGAA GACTCTTATT
    TCAAGCTCTG ATGATTCTTC AATTCAGGTA TGGAATTGGC AGTCAGAGGA ATATGTCTTC
    CTACAAGCCC ATCAGGAAAC TGTGAAAGAC TTTAAGCTCC TGAAAAATTC AAAACTGCTT
    TCCTGGTCAT TTGATGGAAC AGTAAAGATA TGGAGTATTA TTACTGGAAG AATAGAAAAA
    GACTTTGTCT GTCACCAGGA TACAGTACTT TCTTGTGATA TTTCTCCTGA TGCTACCAAG
    TTTTCATCTA CATCTGCAGA TAAGACTGCG AAGATTTGGA GTTTTGAACT TCTTTCACCT
    CTTCATGAAT TGAGGGGCCA CAAGGGCTGT GTGCGCTGCT CTGTCTTCTC TGTGGACAGT
    ACCCTGTTGG CGACTGGAGA TGACAATGGA GAAATCAGGA TATGGAATGT CTCAAATGGT
    GAGCTGCTTC ATTTGTGTGC TCCGATTTTG GTAGAAGAAG GAGCTGCTAC CCATGGAGGC
    TGGGTGACTG ACCTTTGCTT TTCTCCGGAT AGCAAAATGC TTGTCTCTGC TGGAGGATAT
    CTTAAGTGGT GGAATGTTGT CACTGGGGAA TCCTCACAAA CCTTCTACAC AAATGGAACC
    TGTCTTAAGA AAATACACGT TTCCTCTGAC TTCAAAACAT ATGTGACTGT TGATAATCTT
    GGGATTTTAT ATATTTTACA GATGTTAGAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe17605
    Clone ID Related Accession (Same CDS sequence) XM_014119297.1
    Accession Version XM_014119297.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3591bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product apoptotic protease-activating factor 1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTA TTTGACATTC ATACTGGTGG CTTATTGGCA
    GAAATCCATA CAGGCCATCA CAGCACCATC CAGTACTGTG ACTTCTCCCC CCAAAATCAT
    TTGGCAGTAG TGGCTTTGTC CCAGTGCTGT GTGGAGTTGT GGAACATGGA CTCCTGTTTA
    AAAGTGGCTG ATTGCAGAGG ACATTTAAGT TGGGTTCATT GTGTGATGTT TTCTCCCGAT
    GGATCATCAT TTTTGACATC TTCTGATGAC CAGACAATCA GGCTCTGGGA GACCAAAAAA
    GTATGTAAGA ACTCTGCTAT AGTGTTAAAG CAAGAAATAG ATGTTGTGTT TCAAGAAAAT
    GAAGTGATGG TTCTTGCAGT TGACAATATA AAACGTCTGC AGCTCATTAA TGGAAAAACA
    GGTCAGATTG ATTATCTGAT TGAAGCTCAG GTTAGTTGCT GTTGCCTAAG TCCACATCTT
    CAGTACATTG CATTTGGAGG TGAAGATGGA GCCGTTGAGA TTTTAGAACT CCTAAACAAC
    AGAATCTTCC AATCCAGGAT TGGGCACAAG AAAACTGTGC GGCACATCCA GTTCACAGAT
    GATGGGAAGA CTCTTATTTC AAGCTCTGAT GATTCTTCAA TTCAGGTATG GAATTGGCAG
    TCAGAGGAAT ATGTCTTCCT ACAAGCCCAT CAGGAAACTG TGAAAGACTT TAAGCTCCTG
    AAAAATTCAA AACTGCTTTC CTGGTCATTT GATGGAACAG TAAAGATATG GAGTATTATT
    ACTGGAAGAA TAGAAAAAGA CTTTGTCTGT CACCAGGATA CAGTACTTTC TTGTGATATT
    TCTCCTGATG CTACCAAGTT TTCATCTACA TCTGCAGATA AGACTGCGAA GATTTGGAGT
    TTTGAACTTC TTTCACCTCT TCATGAATTG AGGGGCCACA AGGGCTGTGT GCGCTGCTCT
    GTCTTCTCTG TGGACAGTAC CCTGTTGGCG ACTGGAGATG ACAATGGAGA AATCAGGATA
    TGGAATGTCT CAAATGGTGA GCTGCTTCAT TTGTGTGCTC CGATTTTGGT AGAAGAAGGA
    GCTGCTACCC ATGGAGGCTG GGTGACTGAC CTTTGCTTTT CTCCGGATAG CAAAATGCTT
    GTCTCTGCTG GAGGATATCT TAAGTGGTGG AATGTTGTCA CTGGGGAATC CTCACAAACC
    TTCTACACAA ATGGAACCTG TCTTAAGAAA ATACACGTTT CCTCTGACTT CAAAACATAT
    GTGACTGTTG ATAATCTTGG GATTTTATAT ATTTTACAGA TGTTAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974772.1
    CDS172..3762
    Translation

    Target ORF information:

    RefSeq Version XM_014119297.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014119297.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGTG
    TTATGTATTC TCTGGGACAT GGAAACTGAA GAAGTTGAAG ATATTCTGCA GGAGTTTGTT
    AATAAGTCTC TCTTATTCTG TGATCGGAAT GGAAAATCAT TTCGTTATTA TTTACATGAT
    CTTCAAGTAG ATTTCCTTAT AGAGAAGAAT CGCAATCAAC TTCAGGATCT ACATAAGAAG
    ATAGTCACTC AGTTCCAGAA ACATTACCAG CCACATACTC TTTCACCAGA TCAGGAGGAC
    TGCATGTACT GGTACAATTT TCTGGCCTAC CACATGGCTA GTGCCAGTAT GCACAAAGAA
    CTTTGTGCTT TAATGTTTTC CTTGGATTGG ATTAAAGCAA AAACTGAACT CGTAGGCCCT
    GCTCATCTGA TTCATGAATT TGTGGAATAC AGGCATATAT TGGATGAAAA GGATTGTGCA
    GTCTGTGAAA ATTTCCAGGA ATTTTTATCT TTAAATGGAC ATCTTCTTGG ACGACAGCCA
    TTTCCTAATA TTGTACAACT GGGTCTCTGT GAACCAGAAA CTTCAGAAGT TTATCAGCAA
    GCTAAGCTGC AGGCCAAGCA GGAGGTCGAT CACGGAATGC TTTACCTGGA GTGGATAAAC
    AAAAAAAACA TCAAGAATCT TTCCCGCTTA GTTGTCCGCC CCCATACAGA TGCTGTTTAC
    CATGCTTGCT TTTCTGAGGA TGGTCAAAGA ATAGCTTCTT GTGGAGTTGA TAAAACCTTA
    CAGGTGTTCA AAGCTGAAAC AGGAGAGAAA CTGCTAGAAA TCAAGGCTCA TGAAGATGAA
    GTGCTTTGTT GTGCATTCTC TACAGATGAC AGATTTATAG CAACCTGCTC AGTGGATAAA
    AAAGTGAAGA TTTGGAATTC TATGACAGGG GGACTAGTAC ATGTCTATGA TGAGCACTCA
    GAGCAAGTCA ACTGCTGCCA CTTTACCAAC AACAGTCATT ACCTTCTCTT AGCCACTGCA
    TCAAGTGACT GCTTCCTCAA ACTCTGGGAT TTGAATCAAA AAGAATGTCG AAATACCATG
    TTTGGCCATA CAAATTCAGT CAATCACTGC AGATTTTCAC CAGATGATAA GCTTTTGGCT
    AGTTGTTCAG CTGATGGAAC ATTAAAGCTA TTTGACATTC ATACTGGTGG CTTATTGGCA
    GAAATCCATA CAGGCCATCA CAGCACCATC CAGTACTGTG ACTTCTCCCC CCAAAATCAT
    TTGGCAGTAG TGGCTTTGTC CCAGTGCTGT GTGGAGTTGT GGAACATGGA CTCCTGTTTA
    AAAGTGGCTG ATTGCAGAGG ACATTTAAGT TGGGTTCATT GTGTGATGTT TTCTCCCGAT
    GGATCATCAT TTTTGACATC TTCTGATGAC CAGACAATCA GGCTCTGGGA GACCAAAAAA
    GTATGTAAGA ACTCTGCTAT AGTGTTAAAG CAAGAAATAG ATGTTGTGTT TCAAGAAAAT
    GAAGTGATGG TTCTTGCAGT TGACAATATA AAACGTCTGC AGCTCATTAA TGGAAAAACA
    GGTCAGATTG ATTATCTGAT TGAAGCTCAG GTTAGTTGCT GTTGCCTAAG TCCACATCTT
    CAGTACATTG CATTTGGAGG TGAAGATGGA GCCGTTGAGA TTTTAGAACT CCTAAACAAC
    AGAATCTTCC AATCCAGGAT TGGGCACAAG AAAACTGTGC GGCACATCCA GTTCACAGAT
    GATGGGAAGA CTCTTATTTC AAGCTCTGAT GATTCTTCAA TTCAGGTATG GAATTGGCAG
    TCAGAGGAAT ATGTCTTCCT ACAAGCCCAT CAGGAAACTG TGAAAGACTT TAAGCTCCTG
    AAAAATTCAA AACTGCTTTC CTGGTCATTT GATGGAACAG TAAAGATATG GAGTATTATT
    ACTGGAAGAA TAGAAAAAGA CTTTGTCTGT CACCAGGATA CAGTACTTTC TTGTGATATT
    TCTCCTGATG CTACCAAGTT TTCATCTACA TCTGCAGATA AGACTGCGAA GATTTGGAGT
    TTTGAACTTC TTTCACCTCT TCATGAATTG AGGGGCCACA AGGGCTGTGT GCGCTGCTCT
    GTCTTCTCTG TGGACAGTAC CCTGTTGGCG ACTGGAGATG ACAATGGAGA AATCAGGATA
    TGGAATGTCT CAAATGGTGA GCTGCTTCAT TTGTGTGCTC CGATTTTGGT AGAAGAAGGA
    GCTGCTACCC ATGGAGGCTG GGTGACTGAC CTTTGCTTTT CTCCGGATAG CAAAATGCTT
    GTCTCTGCTG GAGGATATCT TAAGTGGTGG AATGTTGTCA CTGGGGAATC CTCACAAACC
    TTCTACACAA ATGGAACCTG TCTTAAGAAA ATACACGTTT CCTCTGACTT CAAAACATAT
    GTGACTGTTG ATAATCTTGG GATTTTATAT ATTTTACAGA TGTTAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe17606
    Clone ID Related Accession (Same CDS sequence) XM_014119298.1
    Accession Version XM_014119298.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3585bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product apoptotic protease-activating factor 1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGAT
    CTACATAAGA AGATAGTCAC TCAGTTCCAG AAACATTACC AGCCACATAC TCTTTCACCA
    GATCAGGAGG ACTGCATGTA CTGGTACAAT TTTCTGGCCT ACCACATGGC TAGTGCCAGT
    ATGCACAAAG AACTTTGTGC TTTAATGTTT TCCTTGGATT GGATTAAAGC AAAAACTGAA
    CTCGTAGGCC CTGCTCATCT GATTCATGAA TTTGTGGAAT ACAGGCATAT ATTGGATGAA
    AAGGATTGTG CAGTCTGTGA AAATTTCCAG GAATTTTTAT CTTTAAATGG ACATCTTCTT
    GGACGACAGC CATTTCCTAA TATTGTACAA CTGGGTCTCT GTGAACCAGA AACTTCAGAA
    GTTTATCAGC AAGCTAAGCT GCAGGCCAAG CAGGAGGTCG ATCACGGAAT GCTTTACCTG
    GAGTGGATAA ACAAAAAAAA CATCAAGAAT CTTTCCCGCT TAGTTGTCCG CCCCCATACA
    GATGCTGTTT ACCATGCTTG CTTTTCTGAG GATGGTCAAA GAATAGCTTC TTGTGGAGTT
    GATAAAACCT TACAGGTGTT CAAAGCTGAA ACAGGAGAGA AACTGCTAGA AATCAAGGCT
    CATGAAGATG AAGTGCTTTG TTGTGCATTC TCTACAGATG ACAGATTTAT AGCAACCTGC
    TCAGTGGATA AAAAAGTGAA GATTTGGAAT TCTATGACAG GGGGACTAGT ACATGTCTAT
    GATGAGCACT CAGAGCAAGT CAACTGCTGC CACTTTACCA ACAACAGTCA TTACCTTCTC
    TTAGCCACTG CATCAAGTGA CTGCTTCCTC AAACTCTGGG ATTTGAATCA AAAAGAATGT
    CGAAATACCA TGTTTGGCCA TACAAATTCA GTCAATCACT GCAGATTTTC ACCAGATGAT
    AAGCTTTTGG CTAGTTGTTC AGCTGATGGA ACATTAAAGC TTTGGGATGT AAAATCAGCA
    AATGAGAGGA AAAGCATTAA TGTGAAGCAG TTCTTCCTAA ATTCAGAGGA GCCTCAAGAG
    GATATGGAAG TGATAGTGAA ATGTTGTTCA TGGTCTGCTG ATGGCACTAG GATAATGGTG
    GCAGCAAAAA ATAAAATCTT TCTATTTGAC ATTCATACTG GTGGCTTATT GGCAGAAATC
    CATACAGGCC ATCACAGCAC CATCCAGTAC TGTGACTTCT CCCCCCAAAA TCATTTGGCA
    GTAGTGGCTT TGTCCCAGTG CTGTGTGGAG TTGTGGAACA TGGACTCCTG TTTAAAAGTG
    GCTGATTGCA GAGGACATTT AAGTTGGGTT CATTGTGTGA TGTTTTCTCC CGATGGATCA
    TCATTTTTGA CATCTTCTGA TGACCAGACA ATCAGGCTCT GGGAGACCAA AAAAGTATGT
    AAGAACTCTG CTATAGTGTT AAAGCAAGAA ATAGATGTTG TGTTTCAAGA AAATGAAGTG
    ATGGTTCTTG CAGTTGACAA TATAAAACGT CTGCAGCTCA TTAATGGAAA AACAGGTCAG
    ATTGATTATC TGATTGAAGC TCAGGTTAGT TGCTGTTGCC TAAGTCCACA TCTTCAGTAC
    ATTGCATTTG GAGGTGAAGA TGGAGCCGTT GAGATTTTAG AACTCCTAAA CAACAGAATC
    TTCCAATCCA GGATTGGGCA CAAGAAAACT GTGCGGCACA TCCAGTTCAC AGATGATGGG
    AAGACTCTTA TTTCAAGCTC TGATGATTCT TCAATTCAGG TATGGAATTG GCAGTCAGAG
    GAATATGTCT TCCTACAAGC CCATCAGGAA ACTGTGAAAG ACTTTAAGCT CCTGAAAAAT
    TCAAAACTGC TTTCCTGGTC ATTTGATGGA ACAGTAAAGA TATGGAGTAT TATTACTGGA
    AGAATAGAAA AAGACTTTGT CTGTCACCAG GATACAGTAC TTTCTTGTGA TATTTCTCCT
    GATGCTACCA AGTTTTCATC TACATCTGCA GATAAGACTG CGAAGATTTG GAGTTTTGAA
    CTTCTTTCAC CTCTTCATGA ATTGAGGGGC CACAAGGGCT GTGTGCGCTG CTCTGTCTTC
    TCTGTGGACA GTACCCTGTT GGCGACTGGA GATGACAATG GAGAAATCAG GATATGGAAT
    GTCTCAAATG GTGAGCTGCT TCATTTGTGT GCTCCGATTT TGGTAGAAGA AGGAGCTGCT
    ACCCATGGAG GCTGGGTGAC TGACCTTTGC TTTTCTCCGG ATAGCAAAAT GCTTGTCTCT
    GCTGGAGGAT ATCTTAAGTG GTGGAATGTT GTCACTGGGG AATCCTCACA AACCTTCTAC
    ACAAATGGAA CCTGTCTTAA GAAAATACAC GTTTCCTCTG ACTTCAAAAC ATATGTGACT
    GTTGATAATC TTGGGATTTT ATATATTTTA CAGATGTTAG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974773.1
    CDS172..3756
    Translation

    Target ORF information:

    RefSeq Version XM_014119298.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris apoptotic peptidase activating factor 1 (APAF1), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014119298.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGATGCAA AAGCTCGAAA CTGTTTGCTT CAATATAGAG AAGCCTTGGA AAAGGACATC 
    AAAACGTCCT ACATCATGGA TCGTATGATT AATGACGGAG TTTTAACAAT ATCAGAGGAG
    GAAAAAGTAA AAAATGAGCC CACTCAACAG CAACGAGCAG CTCTGCTAAT TAAAACAATA
    CTTAAAAAAG ATAATTATTC CTACATATCA TTCTACAATG CTCTGATACA TGAAGGGTAT
    AAAGATCTTG CTGCCCTTCT CCATAGTGGC ATTCCTGTCA TCTCTTCTTC CAACGGTGGC
    AAAGATTCAG TTGGTGGAAT AACTTCACAT GTGAAGACAG TCCTGTGTGA GGGTGGAGTG
    CCACAGAGGC CAGTTGTTTT TGTTACCAGG AAGAAGCTGG TGAATGCAAT TCGTCAGAAC
    CTATTCAAAC TAAGTGATGA ACCAGGATGG GTTGTCATAT ATGGAATGGC AGGTTGTGGG
    AAGTCTGTTT TAGCTGCAGA AGCTGTTCGA GATCATTTTT TCTTAGATGG CTGTTTCCCA
    GGGGGTGTAC ACTGGGTTTC AGTTGGGAAA CAAGACAAAG CTGGGCTTCT GATGAAATTG
    CAGAATCTTT GCACACGGTT GGATCAGGAT GAGAATTTCT CTCAGAGGCC TCCACTCAAT
    ATTGAAGAAG CTAAAGACCG TCTTCGCCTT CTTATGCTGC GCAAACACCC AAGGTCTCTG
    TTGATCTTGG ATGATATTTG GGATTCCTGG ATATTAAAAG CTTTTGACAA TCAGTGTCAG
    ATTCTTCTTA CAACCAGAGA CAAGAGCGTT ACAGATTCAG TAATGGGTCC TAAATATATA
    GTCGCTGTGG AGAGTGACTT AGGAAAAGAA AAAGGACTTG AAATTCTGTC CCTCTTTGTT
    AATATGAAGA AAGCAGATTT GCCAGAGCAA GCTCATAGTA TTATAAAAGA ATGTAAAGGT
    TCTCCTCTCG TAGTGTCTTT AATTGGTGCA CTTTTACGTG ATTTTCCTAA CCGTTGGGAT
    TACTACCTCA GGCAACTTCA GAATAAGCAA TTTAAGAGAA TAAGGAAATC TTCATTTTAT
    GATTATGAGG CTCTGGATGA AGCCATGTCT ATAAGTGTTG AAATGCTCAG AGAGGACATC
    AAAGATTACT ACACAGATCT TTCCATCCTT CAGAAGGATG TTAAGGTGCC TACAAAGGAT
    CTACATAAGA AGATAGTCAC TCAGTTCCAG AAACATTACC AGCCACATAC TCTTTCACCA
    GATCAGGAGG ACTGCATGTA CTGGTACAAT TTTCTGGCCT ACCACATGGC TAGTGCCAGT
    ATGCACAAAG AACTTTGTGC TTTAATGTTT TCCTTGGATT GGATTAAAGC AAAAACTGAA
    CTCGTAGGCC CTGCTCATCT GATTCATGAA TTTGTGGAAT ACAGGCATAT ATTGGATGAA
    AAGGATTGTG CAGTCTGTGA AAATTTCCAG GAATTTTTAT CTTTAAATGG ACATCTTCTT
    GGACGACAGC CATTTCCTAA TATTGTACAA CTGGGTCTCT GTGAACCAGA AACTTCAGAA
    GTTTATCAGC AAGCTAAGCT GCAGGCCAAG CAGGAGGTCG ATCACGGAAT GCTTTACCTG
    GAGTGGATAA ACAAAAAAAA CATCAAGAAT CTTTCCCGCT TAGTTGTCCG CCCCCATACA
    GATGCTGTTT ACCATGCTTG CTTTTCTGAG GATGGTCAAA GAATAGCTTC TTGTGGAGTT
    GATAAAACCT TACAGGTGTT CAAAGCTGAA ACAGGAGAGA AACTGCTAGA AATCAAGGCT
    CATGAAGATG AAGTGCTTTG TTGTGCATTC TCTACAGATG ACAGATTTAT AGCAACCTGC
    TCAGTGGATA AAAAAGTGAA GATTTGGAAT TCTATGACAG GGGGACTAGT ACATGTCTAT
    GATGAGCACT CAGAGCAAGT CAACTGCTGC CACTTTACCA ACAACAGTCA TTACCTTCTC
    TTAGCCACTG CATCAAGTGA CTGCTTCCTC AAACTCTGGG ATTTGAATCA AAAAGAATGT
    CGAAATACCA TGTTTGGCCA TACAAATTCA GTCAATCACT GCAGATTTTC ACCAGATGAT
    AAGCTTTTGG CTAGTTGTTC AGCTGATGGA ACATTAAAGC TTTGGGATGT AAAATCAGCA
    AATGAGAGGA AAAGCATTAA TGTGAAGCAG TTCTTCCTAA ATTCAGAGGA GCCTCAAGAG
    GATATGGAAG TGATAGTGAA ATGTTGTTCA TGGTCTGCTG ATGGCACTAG GATAATGGTG
    GCAGCAAAAA ATAAAATCTT TCTATTTGAC ATTCATACTG GTGGCTTATT GGCAGAAATC
    CATACAGGCC ATCACAGCAC CATCCAGTAC TGTGACTTCT CCCCCCAAAA TCATTTGGCA
    GTAGTGGCTT TGTCCCAGTG CTGTGTGGAG TTGTGGAACA TGGACTCCTG TTTAAAAGTG
    GCTGATTGCA GAGGACATTT AAGTTGGGTT CATTGTGTGA TGTTTTCTCC CGATGGATCA
    TCATTTTTGA CATCTTCTGA TGACCAGACA ATCAGGCTCT GGGAGACCAA AAAAGTATGT
    AAGAACTCTG CTATAGTGTT AAAGCAAGAA ATAGATGTTG TGTTTCAAGA AAATGAAGTG
    ATGGTTCTTG CAGTTGACAA TATAAAACGT CTGCAGCTCA TTAATGGAAA AACAGGTCAG
    ATTGATTATC TGATTGAAGC TCAGGTTAGT TGCTGTTGCC TAAGTCCACA TCTTCAGTAC
    ATTGCATTTG GAGGTGAAGA TGGAGCCGTT GAGATTTTAG AACTCCTAAA CAACAGAATC
    TTCCAATCCA GGATTGGGCA CAAGAAAACT GTGCGGCACA TCCAGTTCAC AGATGATGGG
    AAGACTCTTA TTTCAAGCTC TGATGATTCT TCAATTCAGG TATGGAATTG GCAGTCAGAG
    GAATATGTCT TCCTACAAGC CCATCAGGAA ACTGTGAAAG ACTTTAAGCT CCTGAAAAAT
    TCAAAACTGC TTTCCTGGTC ATTTGATGGA ACAGTAAAGA TATGGAGTAT TATTACTGGA
    AGAATAGAAA AAGACTTTGT CTGTCACCAG GATACAGTAC TTTCTTGTGA TATTTCTCCT
    GATGCTACCA AGTTTTCATC TACATCTGCA GATAAGACTG CGAAGATTTG GAGTTTTGAA
    CTTCTTTCAC CTCTTCATGA ATTGAGGGGC CACAAGGGCT GTGTGCGCTG CTCTGTCTTC
    TCTGTGGACA GTACCCTGTT GGCGACTGGA GATGACAATG GAGAAATCAG GATATGGAAT
    GTCTCAAATG GTGAGCTGCT TCATTTGTGT GCTCCGATTT TGGTAGAAGA AGGAGCTGCT
    ACCCATGGAG GCTGGGTGAC TGACCTTTGC TTTTCTCCGG ATAGCAAAAT GCTTGTCTCT
    GCTGGAGGAT ATCTTAAGTG GTGGAATGTT GTCACTGGGG AATCCTCACA AACCTTCTAC
    ACAAATGGAA CCTGTCTTAA GAAAATACAC GTTTCCTCTG ACTTCAAAAC ATATGTGACT
    GTTGATAATC TTGGGATTTT ATATATTTTA CAGATGTTAG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.