WFS1 cDNA ORF clone, Canis lupus familiaris(Dog)

The following WFS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WFS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe292972 XM_022426924.1
Latest version!
Canis lupus familiaris wolframin ER transmembrane glycoprotein (WFS1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OCe39782 XM_014118768.2
Latest version!
Canis lupus familiaris wolframin ER transmembrane glycoprotein (WFS1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe39783 XM_014118769.1
Latest version!
Canis lupus familiaris wolframin ER transmembrane glycoprotein (WFS1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe39784 XM_014118770.2
Latest version!
Canis lupus familiaris wolframin ER transmembrane glycoprotein (WFS1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OCe39784 XM_014118772.2
Latest version!
Canis lupus familiaris wolframin ER transmembrane glycoprotein (WFS1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OCe39784 XM_014118773.1
Latest version!
Canis lupus familiaris Wolfram syndrome 1 (wolframin) (WFS1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OCe39784 XM_014118771.2
Latest version!
Canis lupus familiaris wolframin ER transmembrane glycoprotein (WFS1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
View Old Accession Versions >>
OCe39784 XM_014118772.1 Canis lupus familiaris Wolfram syndrome 1 (wolframin) (WFS1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OCe39782 XM_014118768.1 Canis lupus familiaris Wolfram syndrome 1 (wolframin) (WFS1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe39784 XM_014118771.1 Canis lupus familiaris Wolfram syndrome 1 (wolframin) (WFS1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OCe39784 XM_014118770.1 Canis lupus familiaris Wolfram syndrome 1 (wolframin) (WFS1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe292972
    Clone ID Related Accession (Same CDS sequence) XM_022426924.1
    Accession Version XM_022426924.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2277bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product wolframin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGACTTGGA AGATGTTTGG AAGGATGGAA AGCCCTGTCC CTATCTCCGG CCTCACTGTC 
    CTGGGCATCA CTTTTGAGAA TGAGCAGGAG GTGAAGCAGC TCTCCTCTGA GACCGACCTG
    GGAGCGGGGC GGTGCGCAAG GCGGGCCCTG GTCATGTACT GGGAAGCTCA ACCCCCAAGA
    AGAAAGAAGC AGGTGGCCGT GTCCGAGCTG CTGGAGAACG TGGGTCAGGT CAACGAGCAG
    GAGGGAGCGG CACAGCCGGG CCCCATCCCC AAGTCCCTCC AGAAGCAGAG GCGGGTGCTG
    GAGCGCTTGG TCAGCAGCGA ATCCAAGAGC CACATAGCGC CGGACGACTT CGTGGAGATC
    ACCAAGAAGT ACGCCAAGGG CATCATCCCC GCCAACCTGT TCCTGCAGGA CGACGACGAC
    GACGAGCTGG CCGGCAAGGG CCCCGAGGAC CTGCCCCTGC GTCTGAAGAT GGTCAAGTAC
    CCGTTGCACG CTGTCATGGA GATCAAGGAG TACCTGATCG ATGTGGCCTC GCGGGCGGGC
    ATGCACTGGC TGTCCAGCAT CGTGCCCACC CAGCACATCA ACGCCCTCAT CTTCTTCTTC
    ATCATCAGCA ACCTGACCAT CGACTTCTTC GCCTTCTTCA TCCCGCTGGT CGTCTTCTAC
    CTGTCGTTCG TCTCCATGGT GATCTGCACC CTCAAGGTGT TCCAGGACAG CAGGGCGTGG
    GAGAGCTTCC GCACCCTCAC GGGCCTGCTC CTGCGCTTCG AGCCCAACCT GGACGTGGAG
    CAGGCCGAGG TCAACTTCGG CTGGAACCAC CTGGAGCCCT ACGTGCACTT CCTGCTGTCT
    GTCTTCTTCG TCATCTTCTC CTTCCCCATC GCCAGCAAGG ACTGCATCCC CTGCTCTGAG
    CTGGCCGTCG TGTCCATCTT CTTCACCGCC ACCAGCTACG TGAGCCTGAG CTCGTCTGCC
    GAGCCCTACA CCCGGAGGGC CCTGGTGACC GAGGTGGCCG CCGGCCTACT GTCCCTCCTG
    CCCACCCTGC CCTGCGGCTG GCAGCACCTG AAGCTCCTGG GCCAGACCTT CGTCACCGTG
    CCCCTGGGCC ACTTGGTCGT CCTGAACGTC AGCGTCCCCT GCCTGCTGTA CGTGTACCTC
    CTGTACCTCT TCTTCCGCAT GGCGCAGCTG CGGGGCTTCA AGGGCACCTA CTGCTACCTG
    GTGCCCTACC TGGTCTGCTT TATGTGGTGT GAGCTGTCCG TGGTCATCCT GCTCGAGTCC
    ACCGGCCTGG GGCTTGCCCG AGCCACCGTG GGCTACTTCC TCTTCCTGTT CGCGCTCCCC
    GTCCTGGTGG CCGGCCTGGC GCTCATGGGC GTGCTGCAGC TTGCCCGCTG GTTCCTGTCG
    CTGGAGCTCA CCAAGGTGGC CGTCACTGCG CTGCTGTGCA GCGTCCCCCT GCTGTTCCGC
    TGGTGGAGCA AGGCCAGCTT CTCGCCGGTG GCCATGGTCA AGTCCCTGAC GCGGAGCTCC
    GTCGTCAAGC TCATCCTGGT GTGGATCACG GCCATCGTGC TCTTCTGCTG GCTGTATGTG
    TACCGCTCGG AGGGCATGAA GGTCTACAAC TCCACGCTCA CCTGGCAGCA GTACGGCTTC
    CTGTGCGGGC CCCGGGCCTG GAAGGAGACC AACATGGCCC GCACCCAGAT CCTGTGCAGC
    CACCTGGAAG GCCACAGGGT CACATGGACG GGCCGCTTCA AGTACGTGCG GGTGACGGAG
    ATCGACAACA GCGCCGAGGC GGCCATCAAC ATGCTCCCGC TCTTCGTGGG CGACTGGATG
    CGCTGCCTGT ACGGCGAGGC CTACCCGTCC TGCAGCCCCG GCAATGCCTC CACGGCCGCG
    GAGGAGCTAT GCCGCCTGAA GCTCCTGGCC AAGCACCCCT GCCACATCCG CAAGTTCGAC
    CGCTACAAGT TCGAGATCAC GGTGGGCATG CCCTACAGTG GCGCCAACGG CTCCCGCGGC
    CCAGAGGAGG ACGACGTCAC CAAGGACATC GTGCTGCGGG CCAGCGGCGA GTTCAAGGAC
    GTGCTTCTCG GCCTGCGCCA CGGCAGCCTC ATCGAGTTCA GCACCGTCCT CGAGGGCCGC
    CTGGGCAGCA AGTGGCCCGT CTTCGAGCTC AAGGCCATCC GCTGCCTCAA CTGCATGGCA
    CAGCTGTCCC CCGCCCGGCG CCACGTGAAG ATAGAGCACG ACTGGCGCAG CACCGTGCAC
    GGCGCCGTCA AGTTCGCCTT TGACTTCTTC TTCTTCCCGT TCCTGTCGGC CGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022282632.1
    CDS867..3143
    Translation

    Target ORF information:

    RefSeq Version XM_022426924.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris wolframin ER transmembrane glycoprotein (WFS1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426924.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGACTTGGA AGATGTTTGG AAGGATGGAA AGCCCTGTCC CTATCTCCGG CCTCACTGTC 
    CTGGGCATCA CTTTTGAGAA TGAGCAGGAG GTGAAGCAGC TCTCCTCTGA GACCGACCTG
    GGAGCGGGGC GGTGCGCAAG GCGGGCCCTG GTCATGTACT GGGAAGCTCA ACCCCCAAGA
    AGAAAGAAGC AGGTGGCCGT GTCCGAGCTG CTGGAGAACG TGGGTCAGGT CAACGAGCAG
    GAGGGAGCGG CACAGCCGGG CCCCATCCCC AAGTCCCTCC AGAAGCAGAG GCGGGTGCTG
    GAGCGCTTGG TCAGCAGCGA ATCCAAGAGC CACATAGCGC CGGACGACTT CGTGGAGATC
    ACCAAGAAGT ACGCCAAGGG CATCATCCCC GCCAACCTGT TCCTGCAGGA CGACGACGAC
    GACGAGCTGG CCGGCAAGGG CCCCGAGGAC CTGCCCCTGC GTCTGAAGAT GGTCAAGTAC
    CCGTTGCACG CTGTCATGGA GATCAAGGAG TACCTGATCG ATGTGGCCTC GCGGGCGGGC
    ATGCACTGGC TGTCCAGCAT CGTGCCCACC CAGCACATCA ACGCCCTCAT CTTCTTCTTC
    ATCATCAGCA ACCTGACCAT CGACTTCTTC GCCTTCTTCA TCCCGCTGGT CGTCTTCTAC
    CTGTCGTTCG TCTCCATGGT GATCTGCACC CTCAAGGTGT TCCAGGACAG CAGGGCGTGG
    GAGAGCTTCC GCACCCTCAC GGGCCTGCTC CTGCGCTTCG AGCCCAACCT GGACGTGGAG
    CAGGCCGAGG TCAACTTCGG CTGGAACCAC CTGGAGCCCT ACGTGCACTT CCTGCTGTCT
    GTCTTCTTCG TCATCTTCTC CTTCCCCATC GCCAGCAAGG ACTGCATCCC CTGCTCTGAG
    CTGGCCGTCG TGTCCATCTT CTTCACCGCC ACCAGCTACG TGAGCCTGAG CTCGTCTGCC
    GAGCCCTACA CCCGGAGGGC CCTGGTGACC GAGGTGGCCG CCGGCCTACT GTCCCTCCTG
    CCCACCCTGC CCTGCGGCTG GCAGCACCTG AAGCTCCTGG GCCAGACCTT CGTCACCGTG
    CCCCTGGGCC ACTTGGTCGT CCTGAACGTC AGCGTCCCCT GCCTGCTGTA CGTGTACCTC
    CTGTACCTCT TCTTCCGCAT GGCGCAGCTG CGGGGCTTCA AGGGCACCTA CTGCTACCTG
    GTGCCCTACC TGGTCTGCTT TATGTGGTGT GAGCTGTCCG TGGTCATCCT GCTCGAGTCC
    ACCGGCCTGG GGCTTGCCCG AGCCACCGTG GGCTACTTCC TCTTCCTGTT CGCGCTCCCC
    GTCCTGGTGG CCGGCCTGGC GCTCATGGGC GTGCTGCAGC TTGCCCGCTG GTTCCTGTCG
    CTGGAGCTCA CCAAGGTGGC CGTCACTGCG CTGCTGTGCA GCGTCCCCCT GCTGTTCCGC
    TGGTGGAGCA AGGCCAGCTT CTCGCCGGTG GCCATGGTCA AGTCCCTGAC GCGGAGCTCC
    GTCGTCAAGC TCATCCTGGT GTGGATCACG GCCATCGTGC TCTTCTGCTG GCTGTATGTG
    TACCGCTCGG AGGGCATGAA GGTCTACAAC TCCACGCTCA CCTGGCAGCA GTACGGCTTC
    CTGTGCGGGC CCCGGGCCTG GAAGGAGACC AACATGGCCC GCACCCAGAT CCTGTGCAGC
    CACCTGGAAG GCCACAGGGT CACATGGACG GGCCGCTTCA AGTACGTGCG GGTGACGGAG
    ATCGACAACA GCGCCGAGGC GGCCATCAAC ATGCTCCCGC TCTTCGTGGG CGACTGGATG
    CGCTGCCTGT ACGGCGAGGC CTACCCGTCC TGCAGCCCCG GCAATGCCTC CACGGCCGCG
    GAGGAGCTAT GCCGCCTGAA GCTCCTGGCC AAGCACCCCT GCCACATCCG CAAGTTCGAC
    CGCTACAAGT TCGAGATCAC GGTGGGCATG CCCTACAGTG GCGCCAACGG CTCCCGCGGC
    CCAGAGGAGG ACGACGTCAC CAAGGACATC GTGCTGCGGG CCAGCGGCGA GTTCAAGGAC
    GTGCTTCTCG GCCTGCGCCA CGGCAGCCTC ATCGAGTTCA GCACCGTCCT CGAGGGCCGC
    CTGGGCAGCA AGTGGCCCGT CTTCGAGCTC AAGGCCATCC GCTGCCTCAA CTGCATGGCA
    CAGCTGTCCC CCGCCCGGCG CCACGTGAAG ATAGAGCACG ACTGGCGCAG CACCGTGCAC
    GGCGCCGTCA AGTTCGCCTT TGACTTCTTC TTCTTCCCGT TCCTGTCGGC CGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39782
    Clone ID Related Accession (Same CDS sequence) XM_014118768.1 , XM_014118768.2
    Accession Version XM_014118768.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2712bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product wolframin isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726083.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGACGCCA GCACCCCCCC TACGAGCCCC TCTGGCCCAC AGCCTCCACC ACCGCAGCCC 
    CAGGCCCGCT CGCGGCTCAA TGCCACTGCC TCGGTGGAAC AGGACGAGAG GGGGGCGCCC
    CGTGCTCCGG GACCAGACGC TGCCAATGCG GCTGCCCCCG CTGCAGCCCG GGCCCCAAGT
    GCTCGGAGCC AGGAAAGGGC GGACAGAGCA GGTAAGGGGC TGGGGCTGCA AGCTGACCAT
    CCTCAGTGCC ACCAGCGCTG CGTGAAGCCA GGGCCCATGA AGGCAGATGT GGAGGTGCCC
    TTTGGAGACG TGCTGGAGAG GGCCAAGGCA GGGGACGCCA AGGCGCAGAC GGAGGCGGGG
    AAGCACTACC TGCTCCTGGC GGGCACCGGG GACGAGGAGC TGAACAACTG CACGGCTGTC
    GACTGGCTCA TCCTCGCTGC CAAGCAGGGC CGGCGCGAGG CTGTCAAGCT CCTGCGCAGG
    TGCCTGGCCG ACAGGAAAGG CATCACTTTT GAGAATGAGC AGGAGGTGAA GCAGCTCTCC
    TCTGAGACCG ACCTGGGAGC GGGGCGGTGC GCAAGGCGGG CCCTGGTCAT GTACTGGGAA
    GCTCAACCCC CAAGAAGAAA GAAGCAGGTG GCCGTGTCCG AGCTGCTGGA GAACGTGGGT
    CAGGTCAACG AGCAGGAGGG AGCGGCACAG CCGGGCCCCA TCCCCAAGTC CCTCCAGAAG
    CAGAGGCGGG TGCTGGAGCG CTTGGTCAGC AGCGAATCCA AGAGCCACAT AGCGCCGGAC
    GACTTCGTGG AGATCACCAA GAAGTACGCC AAGGGCATCA TCCCCGCCAA CCTGTTCCTG
    CAGGACGACG ACGACGACGA GCTGGCCGGC AAGGGCCCCG AGGACCTGCC CCTGCGTCTG
    AAGATGGTCA AGTACCCGTT GCACGCTGTC ATGGAGATCA AGGAGTACCT GATCGATGTG
    GCCTCGCGGG CGGGCATGCA CTGGCTGTCC AGCATCGTGC CCACCCAGCA CATCAACGCC
    CTCATCTTCT TCTTCATCAT CAGCAACCTG ACCATCGACT TCTTCGCCTT CTTCATCCCG
    CTGGTCGTCT TCTACCTGTC GTTCGTCTCC ATGGTGATCT GCACCCTCAA GGTGTTCCAG
    GACAGCAGGG CGTGGGAGAG CTTCCGCACC CTCACGGGCC TGCTCCTGCG CTTCGAGCCC
    AACCTGGACG TGGAGCAGGC CGAGGTCAAC TTCGGCTGGA ACCACCTGGA GCCCTACGTG
    CACTTCCTGC TGTCTGTCTT CTTCGTCATC TTCTCCTTCC CCATCGCCAG CAAGGACTGC
    ATCCCCTGCT CTGAGCTGGC CGTCGTGTCC ATCTTCTTCA CCGCCACCAG CTACGTGAGC
    CTGAGCTCGT CTGCCGAGCC CTACACCCGG AGGGCCCTGG TGACCGAGGT GGCCGCCGGC
    CTACTGTCCC TCCTGCCCAC CCTGCCCTGC GGCTGGCAGC ACCTGAAGCT CCTGGGCCAG
    ACCTTCGTCA CCGTGCCCCT GGGCCACTTG GTCGTCCTGA ACGTCAGCGT CCCCTGCCTG
    CTGTACGTGT ACCTCCTGTA CCTCTTCTTC CGCATGGCGC AGCTGCGGGG CTTCAAGGGC
    ACCTACTGCT ACCTGGTGCC CTACCTGGTC TGCTTTATGT GGTGTGAGCT GTCCGTGGTC
    ATCCTGCTCG AGTCCACCGG CCTGGGGCTT GCCCGAGCCA CCGTGGGCTA CTTCCTCTTC
    CTGTTCGCGC TCCCCGTCCT GGTGGCCGGC CTGGCGCTCA TGGGCGTGCT GCAGCTTGCC
    CGCTGGTTCC TGTCGCTGGA GCTCACCAAG GTGGCCGTCA CTGCGCTGCT GTGCAGCGTC
    CCCCTGCTGT TCCGCTGGTG GAGCAAGGCC AGCTTCTCGC CGGTGGCCAT GGTCAAGTCC
    CTGACGCGGA GCTCCGTCGT CAAGCTCATC CTGGTGTGGA TCACGGCCAT CGTGCTCTTC
    TGCTGGCTGT ATGTGTACCG CTCGGAGGGC ATGAAGGTCT ACAACTCCAC GCTCACCTGG
    CAGCAGTACG GCTTCCTGTG CGGGCCCCGG GCCTGGAAGG AGACCAACAT GGCCCGCACC
    CAGATCCTGT GCAGCCACCT GGAAGGCCAC AGGGTCACAT GGACGGGCCG CTTCAAGTAC
    GTGCGGGTGA CGGAGATCGA CAACAGCGCC GAGGCGGCCA TCAACATGCT CCCGCTCTTC
    GTGGGCGACT GGATGCGCTG CCTGTACGGC GAGGCCTACC CGTCCTGCAG CCCCGGCAAT
    GCCTCCACGG CCGCGGAGGA GCTATGCCGC CTGAAGCTCC TGGCCAAGCA CCCCTGCCAC
    ATCCGCAAGT TCGACCGCTA CAAGTTCGAG ATCACGGTGG GCATGCCCTA CAGTGGCGCC
    AACGGCTCCC GCGGCCCAGA GGAGGACGAC GTCACCAAGG ACATCGTGCT GCGGGCCAGC
    GGCGAGTTCA AGGACGTGCT TCTCGGCCTG CGCCACGGCA GCCTCATCGA GTTCAGCACC
    GTCCTCGAGG GCCGCCTGGG CAGCAAGTGG CCCGTCTTCG AGCTCAAGGC CATCCGCTGC
    CTCAACTGCA TGGCACAGCT GTCCCCCGCC CGGCGCCACG TGAAGATAGA GCACGACTGG
    CGCAGCACCG TGCACGGCGC CGTCAAGTTC GCCTTTGACT TCTTCTTCTT CCCGTTCCTG
    TCGGCCGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974243.1
    CDS2..2713
    Translation

    Target ORF information:

    RefSeq Version XM_014118768.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Wolfram syndrome 1 (wolframin) (WFS1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118768.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGACGCCA GCACCCCCCC TACGAGCCCC TCTGGCCCAC AGCCTCCACC ACCGCAGCCC 
    CAGGCCCGCT CGCGGCTCAA TGCCACTGCC TCGGTGGAAC AGGACGAGAG GGGGGCGCCC
    CGTGCTCCGG GACCAGACGC TGCCAATGCG GCTGCCCCCG CTGCAGCCCG GGCCCCAAGT
    GCTCGGAGCC AGGAAAGGGC GGACAGAGCA GGTAAGGGGC TGGGGCTGCA AGCTGACCAT
    CCTCAGTGCC ACCAGCGCTG CGTGAAGCCA GGGCCCATGA AGGCAGATGT GGAGGTGCCC
    TTTGGAGACG TGCTGGAGAG GGCCAAGGCA GGGGACGCCA AGGCGCAGAC GGAGGCGGGG
    AAGCACTACC TGCTCCTGGC GGGCACCGGG GACGAGGAGC TGAACAACTG CACGGCTGTC
    GACTGGCTCA TCCTCGCTGC CAAGCAGGGC CGGCGCGAGG CTGTCAAGCT CCTGCGCAGG
    TGCCTGGCCG ACAGGAAAGG CATCACTTTT GAGAATGAGC AGGAGGTGAA GCAGCTCTCC
    TCTGAGACCG ACCTGGGAGC GGGGCGGTGC GCAAGGCGGG CCCTGGTCAT GTACTGGGAA
    GCTCAACCCC CAAGAAGAAA GAAGCAGGTG GCCGTGTCCG AGCTGCTGGA GAACGTGGGT
    CAGGTCAACG AGCAGGAGGG AGCGGCACAG CCGGGCCCCA TCCCCAAGTC CCTCCAGAAG
    CAGAGGCGGG TGCTGGAGCG CTTGGTCAGC AGCGAATCCA AGAGCCACAT AGCGCCGGAC
    GACTTCGTGG AGATCACCAA GAAGTACGCC AAGGGCATCA TCCCCGCCAA CCTGTTCCTG
    CAGGACGACG ACGACGACGA GCTGGCCGGC AAGGGCCCCG AGGACCTGCC CCTGCGTCTG
    AAGATGGTCA AGTACCCGTT GCACGCTGTC ATGGAGATCA AGGAGTACCT GATCGATGTG
    GCCTCGCGGG CGGGCATGCA CTGGCTGTCC AGCATCGTGC CCACCCAGCA CATCAACGCC
    CTCATCTTCT TCTTCATCAT CAGCAACCTG ACCATCGACT TCTTCGCCTT CTTCATCCCG
    CTGGTCGTCT TCTACCTGTC GTTCGTCTCC ATGGTGATCT GCACCCTCAA GGTGTTCCAG
    GACAGCAGGG CGTGGGAGAG CTTCCGCACC CTCACGGGCC TGCTCCTGCG CTTCGAGCCC
    AACCTGGACG TGGAGCAGGC CGAGGTCAAC TTCGGCTGGA ACCACCTGGA GCCCTACGTG
    CACTTCCTGC TGTCTGTCTT CTTCGTCATC TTCTCCTTCC CCATCGCCAG CAAGGACTGC
    ATCCCCTGCT CTGAGCTGGC CGTCGTGTCC ATCTTCTTCA CCGCCACCAG CTACGTGAGC
    CTGAGCTCGT CTGCCGAGCC CTACACCCGG AGGGCCCTGG TGACCGAGGT GGCCGCCGGC
    CTACTGTCCC TCCTGCCCAC CCTGCCCTGC GGCTGGCAGC ACCTGAAGCT CCTGGGCCAG
    ACCTTCGTCA CCGTGCCCCT GGGCCACTTG GTCGTCCTGA ACGTCAGCGT CCCCTGCCTG
    CTGTACGTGT ACCTCCTGTA CCTCTTCTTC CGCATGGCGC AGCTGCGGGG CTTCAAGGGC
    ACCTACTGCT ACCTGGTGCC CTACCTGGTC TGCTTTATGT GGTGTGAGCT GTCCGTGGTC
    ATCCTGCTCG AGTCCACCGG CCTGGGGCTT GCCCGAGCCA CCGTGGGCTA CTTCCTCTTC
    CTGTTCGCGC TCCCCGTCCT GGTGGCCGGC CTGGCGCTCA TGGGCGTGCT GCAGCTTGCC
    CGCTGGTTCC TGTCGCTGGA GCTCACCAAG GTGGCCGTCA CTGCGCTGCT GTGCAGCGTC
    CCCCTGCTGT TCCGCTGGTG GAGCAAGGCC AGCTTCTCGC CGGTGGCCAT GGTCAAGTCC
    CTGACGCGGA GCTCCGTCGT CAAGCTCATC CTGGTGTGGA TCACGGCCAT CGTGCTCTTC
    TGCTGGCTGT ATGTGTACCG CTCGGAGGGC ATGAAGGTCT ACAACTCCAC GCTCACCTGG
    CAGCAGTACG GCTTCCTGTG CGGGCCCCGG GCCTGGAAGG AGACCAACAT GGCCCGCACC
    CAGATCCTGT GCAGCCACCT GGAAGGCCAC AGGGTCACAT GGACGGGCCG CTTCAAGTAC
    GTGCGGGTGA CGGAGATCGA CAACAGCGCC GAGGCGGCCA TCAACATGCT CCCGCTCTTC
    GTGGGCGACT GGATGCGCTG CCTGTACGGC GAGGCCTACC CGTCCTGCAG CCCCGGCAAT
    GCCTCCACGG CCGCGGAGGA GCTATGCCGC CTGAAGCTCC TGGCCAAGCA CCCCTGCCAC
    ATCCGCAAGT TCGACCGCTA CAAGTTCGAG ATCACGGTGG GCATGCCCTA CAGTGGCGCC
    AACGGCTCCC GCGGCCCAGA GGAGGACGAC GTCACCAAGG ACATCGTGCT GCGGGCCAGC
    GGCGAGTTCA AGGACGTGCT TCTCGGCCTG CGCCACGGCA GCCTCATCGA GTTCAGCACC
    GTCCTCGAGG GCCGCCTGGG CAGCAAGTGG CCCGTCTTCG AGCTCAAGGC CATCCGCTGC
    CTCAACTGCA TGGCACAGCT GTCCCCCGCC CGGCGCCACG TGAAGATAGA GCACGACTGG
    CGCAGCACCG TGCACGGCGC CGTCAAGTTC GCCTTTGACT TCTTCTTCTT CCCGTTCCTG
    TCGGCCGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39782
    Clone ID Related Accession (Same CDS sequence) XM_014118768.1 , XM_014118768.2
    Accession Version XM_014118768.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2712bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product wolframin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014118768.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGACGCCA GCACCCCCCC TACGAGCCCC TCTGGCCCAC AGCCTCCACC ACCGCAGCCC 
    CAGGCCCGCT CGCGGCTCAA TGCCACTGCC TCGGTGGAAC AGGACGAGAG GGGGGCGCCC
    CGTGCTCCGG GACCAGACGC TGCCAATGCG GCTGCCCCCG CTGCAGCCCG GGCCCCAAGT
    GCTCGGAGCC AGGAAAGGGC GGACAGAGCA GGTAAGGGGC TGGGGCTGCA AGCTGACCAT
    CCTCAGTGCC ACCAGCGCTG CGTGAAGCCA GGGCCCATGA AGGCAGATGT GGAGGTGCCC
    TTTGGAGACG TGCTGGAGAG GGCCAAGGCA GGGGACGCCA AGGCGCAGAC GGAGGCGGGG
    AAGCACTACC TGCTCCTGGC GGGCACCGGG GACGAGGAGC TGAACAACTG CACGGCTGTC
    GACTGGCTCA TCCTCGCTGC CAAGCAGGGC CGGCGCGAGG CTGTCAAGCT CCTGCGCAGG
    TGCCTGGCCG ACAGGAAAGG CATCACTTTT GAGAATGAGC AGGAGGTGAA GCAGCTCTCC
    TCTGAGACCG ACCTGGGAGC GGGGCGGTGC GCAAGGCGGG CCCTGGTCAT GTACTGGGAA
    GCTCAACCCC CAAGAAGAAA GAAGCAGGTG GCCGTGTCCG AGCTGCTGGA GAACGTGGGT
    CAGGTCAACG AGCAGGAGGG AGCGGCACAG CCGGGCCCCA TCCCCAAGTC CCTCCAGAAG
    CAGAGGCGGG TGCTGGAGCG CTTGGTCAGC AGCGAATCCA AGAGCCACAT AGCGCCGGAC
    GACTTCGTGG AGATCACCAA GAAGTACGCC AAGGGCATCA TCCCCGCCAA CCTGTTCCTG
    CAGGACGACG ACGACGACGA GCTGGCCGGC AAGGGCCCCG AGGACCTGCC CCTGCGTCTG
    AAGATGGTCA AGTACCCGTT GCACGCTGTC ATGGAGATCA AGGAGTACCT GATCGATGTG
    GCCTCGCGGG CGGGCATGCA CTGGCTGTCC AGCATCGTGC CCACCCAGCA CATCAACGCC
    CTCATCTTCT TCTTCATCAT CAGCAACCTG ACCATCGACT TCTTCGCCTT CTTCATCCCG
    CTGGTCGTCT TCTACCTGTC GTTCGTCTCC ATGGTGATCT GCACCCTCAA GGTGTTCCAG
    GACAGCAGGG CGTGGGAGAG CTTCCGCACC CTCACGGGCC TGCTCCTGCG CTTCGAGCCC
    AACCTGGACG TGGAGCAGGC CGAGGTCAAC TTCGGCTGGA ACCACCTGGA GCCCTACGTG
    CACTTCCTGC TGTCTGTCTT CTTCGTCATC TTCTCCTTCC CCATCGCCAG CAAGGACTGC
    ATCCCCTGCT CTGAGCTGGC CGTCGTGTCC ATCTTCTTCA CCGCCACCAG CTACGTGAGC
    CTGAGCTCGT CTGCCGAGCC CTACACCCGG AGGGCCCTGG TGACCGAGGT GGCCGCCGGC
    CTACTGTCCC TCCTGCCCAC CCTGCCCTGC GGCTGGCAGC ACCTGAAGCT CCTGGGCCAG
    ACCTTCGTCA CCGTGCCCCT GGGCCACTTG GTCGTCCTGA ACGTCAGCGT CCCCTGCCTG
    CTGTACGTGT ACCTCCTGTA CCTCTTCTTC CGCATGGCGC AGCTGCGGGG CTTCAAGGGC
    ACCTACTGCT ACCTGGTGCC CTACCTGGTC TGCTTTATGT GGTGTGAGCT GTCCGTGGTC
    ATCCTGCTCG AGTCCACCGG CCTGGGGCTT GCCCGAGCCA CCGTGGGCTA CTTCCTCTTC
    CTGTTCGCGC TCCCCGTCCT GGTGGCCGGC CTGGCGCTCA TGGGCGTGCT GCAGCTTGCC
    CGCTGGTTCC TGTCGCTGGA GCTCACCAAG GTGGCCGTCA CTGCGCTGCT GTGCAGCGTC
    CCCCTGCTGT TCCGCTGGTG GAGCAAGGCC AGCTTCTCGC CGGTGGCCAT GGTCAAGTCC
    CTGACGCGGA GCTCCGTCGT CAAGCTCATC CTGGTGTGGA TCACGGCCAT CGTGCTCTTC
    TGCTGGCTGT ATGTGTACCG CTCGGAGGGC ATGAAGGTCT ACAACTCCAC GCTCACCTGG
    CAGCAGTACG GCTTCCTGTG CGGGCCCCGG GCCTGGAAGG AGACCAACAT GGCCCGCACC
    CAGATCCTGT GCAGCCACCT GGAAGGCCAC AGGGTCACAT GGACGGGCCG CTTCAAGTAC
    GTGCGGGTGA CGGAGATCGA CAACAGCGCC GAGGCGGCCA TCAACATGCT CCCGCTCTTC
    GTGGGCGACT GGATGCGCTG CCTGTACGGC GAGGCCTACC CGTCCTGCAG CCCCGGCAAT
    GCCTCCACGG CCGCGGAGGA GCTATGCCGC CTGAAGCTCC TGGCCAAGCA CCCCTGCCAC
    ATCCGCAAGT TCGACCGCTA CAAGTTCGAG ATCACGGTGG GCATGCCCTA CAGTGGCGCC
    AACGGCTCCC GCGGCCCAGA GGAGGACGAC GTCACCAAGG ACATCGTGCT GCGGGCCAGC
    GGCGAGTTCA AGGACGTGCT TCTCGGCCTG CGCCACGGCA GCCTCATCGA GTTCAGCACC
    GTCCTCGAGG GCCGCCTGGG CAGCAAGTGG CCCGTCTTCG AGCTCAAGGC CATCCGCTGC
    CTCAACTGCA TGGCACAGCT GTCCCCCGCC CGGCGCCACG TGAAGATAGA GCACGACTGG
    CGCAGCACCG TGCACGGCGC CGTCAAGTTC GCCTTTGACT TCTTCTTCTT CCCGTTCCTG
    TCGGCCGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974243.1
    CDS42..2753
    Translation

    Target ORF information:

    RefSeq Version XM_014118768.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris wolframin ER transmembrane glycoprotein (WFS1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118768.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGACGCCA GCACCCCCCC TACGAGCCCC TCTGGCCCAC AGCCTCCACC ACCGCAGCCC 
    CAGGCCCGCT CGCGGCTCAA TGCCACTGCC TCGGTGGAAC AGGACGAGAG GGGGGCGCCC
    CGTGCTCCGG GACCAGACGC TGCCAATGCG GCTGCCCCCG CTGCAGCCCG GGCCCCAAGT
    GCTCGGAGCC AGGAAAGGGC GGACAGAGCA GGTAAGGGGC TGGGGCTGCA AGCTGACCAT
    CCTCAGTGCC ACCAGCGCTG CGTGAAGCCA GGGCCCATGA AGGCAGATGT GGAGGTGCCC
    TTTGGAGACG TGCTGGAGAG GGCCAAGGCA GGGGACGCCA AGGCGCAGAC GGAGGCGGGG
    AAGCACTACC TGCTCCTGGC GGGCACCGGG GACGAGGAGC TGAACAACTG CACGGCTGTC
    GACTGGCTCA TCCTCGCTGC CAAGCAGGGC CGGCGCGAGG CTGTCAAGCT CCTGCGCAGG
    TGCCTGGCCG ACAGGAAAGG CATCACTTTT GAGAATGAGC AGGAGGTGAA GCAGCTCTCC
    TCTGAGACCG ACCTGGGAGC GGGGCGGTGC GCAAGGCGGG CCCTGGTCAT GTACTGGGAA
    GCTCAACCCC CAAGAAGAAA GAAGCAGGTG GCCGTGTCCG AGCTGCTGGA GAACGTGGGT
    CAGGTCAACG AGCAGGAGGG AGCGGCACAG CCGGGCCCCA TCCCCAAGTC CCTCCAGAAG
    CAGAGGCGGG TGCTGGAGCG CTTGGTCAGC AGCGAATCCA AGAGCCACAT AGCGCCGGAC
    GACTTCGTGG AGATCACCAA GAAGTACGCC AAGGGCATCA TCCCCGCCAA CCTGTTCCTG
    CAGGACGACG ACGACGACGA GCTGGCCGGC AAGGGCCCCG AGGACCTGCC CCTGCGTCTG
    AAGATGGTCA AGTACCCGTT GCACGCTGTC ATGGAGATCA AGGAGTACCT GATCGATGTG
    GCCTCGCGGG CGGGCATGCA CTGGCTGTCC AGCATCGTGC CCACCCAGCA CATCAACGCC
    CTCATCTTCT TCTTCATCAT CAGCAACCTG ACCATCGACT TCTTCGCCTT CTTCATCCCG
    CTGGTCGTCT TCTACCTGTC GTTCGTCTCC ATGGTGATCT GCACCCTCAA GGTGTTCCAG
    GACAGCAGGG CGTGGGAGAG CTTCCGCACC CTCACGGGCC TGCTCCTGCG CTTCGAGCCC
    AACCTGGACG TGGAGCAGGC CGAGGTCAAC TTCGGCTGGA ACCACCTGGA GCCCTACGTG
    CACTTCCTGC TGTCTGTCTT CTTCGTCATC TTCTCCTTCC CCATCGCCAG CAAGGACTGC
    ATCCCCTGCT CTGAGCTGGC CGTCGTGTCC ATCTTCTTCA CCGCCACCAG CTACGTGAGC
    CTGAGCTCGT CTGCCGAGCC CTACACCCGG AGGGCCCTGG TGACCGAGGT GGCCGCCGGC
    CTACTGTCCC TCCTGCCCAC CCTGCCCTGC GGCTGGCAGC ACCTGAAGCT CCTGGGCCAG
    ACCTTCGTCA CCGTGCCCCT GGGCCACTTG GTCGTCCTGA ACGTCAGCGT CCCCTGCCTG
    CTGTACGTGT ACCTCCTGTA CCTCTTCTTC CGCATGGCGC AGCTGCGGGG CTTCAAGGGC
    ACCTACTGCT ACCTGGTGCC CTACCTGGTC TGCTTTATGT GGTGTGAGCT GTCCGTGGTC
    ATCCTGCTCG AGTCCACCGG CCTGGGGCTT GCCCGAGCCA CCGTGGGCTA CTTCCTCTTC
    CTGTTCGCGC TCCCCGTCCT GGTGGCCGGC CTGGCGCTCA TGGGCGTGCT GCAGCTTGCC
    CGCTGGTTCC TGTCGCTGGA GCTCACCAAG GTGGCCGTCA CTGCGCTGCT GTGCAGCGTC
    CCCCTGCTGT TCCGCTGGTG GAGCAAGGCC AGCTTCTCGC CGGTGGCCAT GGTCAAGTCC
    CTGACGCGGA GCTCCGTCGT CAAGCTCATC CTGGTGTGGA TCACGGCCAT CGTGCTCTTC
    TGCTGGCTGT ATGTGTACCG CTCGGAGGGC ATGAAGGTCT ACAACTCCAC GCTCACCTGG
    CAGCAGTACG GCTTCCTGTG CGGGCCCCGG GCCTGGAAGG AGACCAACAT GGCCCGCACC
    CAGATCCTGT GCAGCCACCT GGAAGGCCAC AGGGTCACAT GGACGGGCCG CTTCAAGTAC
    GTGCGGGTGA CGGAGATCGA CAACAGCGCC GAGGCGGCCA TCAACATGCT CCCGCTCTTC
    GTGGGCGACT GGATGCGCTG CCTGTACGGC GAGGCCTACC CGTCCTGCAG CCCCGGCAAT
    GCCTCCACGG CCGCGGAGGA GCTATGCCGC CTGAAGCTCC TGGCCAAGCA CCCCTGCCAC
    ATCCGCAAGT TCGACCGCTA CAAGTTCGAG ATCACGGTGG GCATGCCCTA CAGTGGCGCC
    AACGGCTCCC GCGGCCCAGA GGAGGACGAC GTCACCAAGG ACATCGTGCT GCGGGCCAGC
    GGCGAGTTCA AGGACGTGCT TCTCGGCCTG CGCCACGGCA GCCTCATCGA GTTCAGCACC
    GTCCTCGAGG GCCGCCTGGG CAGCAAGTGG CCCGTCTTCG AGCTCAAGGC CATCCGCTGC
    CTCAACTGCA TGGCACAGCT GTCCCCCGCC CGGCGCCACG TGAAGATAGA GCACGACTGG
    CGCAGCACCG TGCACGGCGC CGTCAAGTTC GCCTTTGACT TCTTCTTCTT CCCGTTCCTG
    TCGGCCGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39783
    Clone ID Related Accession (Same CDS sequence) XM_014118769.1
    Accession Version XM_014118769.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2652bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product wolframin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGACGCCA GCACCCCCCC TACGAGCCCC TCTGGCCCAC AGCCTCCACC ACCGCAGCCC 
    CAGGCCCGCT CGCGGCTCAA TGCCACTGCC TCGGTGGAAC AGGACGAGAG GGGGGCGCCC
    CGTGCTCCGG GACCAGACGC TGCCAATGCG GCTGCCCCCG CTGCAGCCCG GGCCCCAAGT
    GCTCGGAGCC AGGAAAGGGC GGACAGAGCA GGGCCCATGA AGGCAGATGT GGAGGTGCCC
    TTTGGAGACG TGCTGGAGAG GGCCAAGGCA GGGGACGCCA AGGCGCAGAC GGAGGCGGGG
    AAGCACTACC TGCTCCTGGC GGGCACCGGG GACGAGGAGC TGAACAACTG CACGGCTGTC
    GACTGGCTCA TCCTCGCTGC CAAGCAGGGC CGGCGCGAGG CTGTCAAGCT CCTGCGCAGG
    TGCCTGGCCG ACAGGAAAGG CATCACTTTT GAGAATGAGC AGGAGGTGAA GCAGCTCTCC
    TCTGAGACCG ACCTGGGAGC GGGGCGGTGC GCAAGGCGGG CCCTGGTCAT GTACTGGGAA
    GCTCAACCCC CAAGAAGAAA GAAGCAGGTG GCCGTGTCCG AGCTGCTGGA GAACGTGGGT
    CAGGTCAACG AGCAGGAGGG AGCGGCACAG CCGGGCCCCA TCCCCAAGTC CCTCCAGAAG
    CAGAGGCGGG TGCTGGAGCG CTTGGTCAGC AGCGAATCCA AGAGCCACAT AGCGCCGGAC
    GACTTCGTGG AGATCACCAA GAAGTACGCC AAGGGCATCA TCCCCGCCAA CCTGTTCCTG
    CAGGACGACG ACGACGACGA GCTGGCCGGC AAGGGCCCCG AGGACCTGCC CCTGCGTCTG
    AAGATGGTCA AGTACCCGTT GCACGCTGTC ATGGAGATCA AGGAGTACCT GATCGATGTG
    GCCTCGCGGG CGGGCATGCA CTGGCTGTCC AGCATCGTGC CCACCCAGCA CATCAACGCC
    CTCATCTTCT TCTTCATCAT CAGCAACCTG ACCATCGACT TCTTCGCCTT CTTCATCCCG
    CTGGTCGTCT TCTACCTGTC GTTCGTCTCC ATGGTGATCT GCACCCTCAA GGTGTTCCAG
    GACAGCAGGG CGTGGGAGAG CTTCCGCACC CTCACGGGCC TGCTCCTGCG CTTCGAGCCC
    AACCTGGACG TGGAGCAGGC CGAGGTCAAC TTCGGCTGGA ACCACCTGGA GCCCTACGTG
    CACTTCCTGC TGTCTGTCTT CTTCGTCATC TTCTCCTTCC CCATCGCCAG CAAGGACTGC
    ATCCCCTGCT CTGAGCTGGC CGTCGTGTCC ATCTTCTTCA CCGCCACCAG CTACGTGAGC
    CTGAGCTCGT CTGCCGAGCC CTACACCCGG AGGGCCCTGG TGACCGAGGT GGCCGCCGGC
    CTACTGTCCC TCCTGCCCAC CCTGCCCTGC GGCTGGCAGC ACCTGAAGCT CCTGGGCCAG
    ACCTTCGTCA CCGTGCCCCT GGGCCACTTG GTCGTCCTGA ACGTCAGCGT CCCCTGCCTG
    CTGTACGTGT ACCTCCTGTA CCTCTTCTTC CGCATGGCGC AGCTGCGGGG CTTCAAGGGC
    ACCTACTGCT ACCTGGTGCC CTACCTGGTC TGCTTTATGT GGTGTGAGCT GTCCGTGGTC
    ATCCTGCTCG AGTCCACCGG CCTGGGGCTT GCCCGAGCCA CCGTGGGCTA CTTCCTCTTC
    CTGTTCGCGC TCCCCGTCCT GGTGGCCGGC CTGGCGCTCA TGGGCGTGCT GCAGCTTGCC
    CGCTGGTTCC TGTCGCTGGA GCTCACCAAG GTGGCCGTCA CTGCGCTGCT GTGCAGCGTC
    CCCCTGCTGT TCCGCTGGTG GAGCAAGGCC AGCTTCTCGC CGGTGGCCAT GGTCAAGTCC
    CTGACGCGGA GCTCCGTCGT CAAGCTCATC CTGGTGTGGA TCACGGCCAT CGTGCTCTTC
    TGCTGGCTGT ATGTGTACCG CTCGGAGGGC ATGAAGGTCT ACAACTCCAC GCTCACCTGG
    CAGCAGTACG GCTTCCTGTG CGGGCCCCGG GCCTGGAAGG AGACCAACAT GGCCCGCACC
    CAGATCCTGT GCAGCCACCT GGAAGGCCAC AGGGTCACAT GGACGGGCCG CTTCAAGTAC
    GTGCGGGTGA CGGAGATCGA CAACAGCGCC GAGGCGGCCA TCAACATGCT CCCGCTCTTC
    GTGGGCGACT GGATGCGCTG CCTGTACGGC GAGGCCTACC CGTCCTGCAG CCCCGGCAAT
    GCCTCCACGG CCGCGGAGGA GCTATGCCGC CTGAAGCTCC TGGCCAAGCA CCCCTGCCAC
    ATCCGCAAGT TCGACCGCTA CAAGTTCGAG ATCACGGTGG GCATGCCCTA CAGTGGCGCC
    AACGGCTCCC GCGGCCCAGA GGAGGACGAC GTCACCAAGG ACATCGTGCT GCGGGCCAGC
    GGCGAGTTCA AGGACGTGCT TCTCGGCCTG CGCCACGGCA GCCTCATCGA GTTCAGCACC
    GTCCTCGAGG GCCGCCTGGG CAGCAAGTGG CCCGTCTTCG AGCTCAAGGC CATCCGCTGC
    CTCAACTGCA TGGCACAGCT GTCCCCCGCC CGGCGCCACG TGAAGATAGA GCACGACTGG
    CGCAGCACCG TGCACGGCGC CGTCAAGTTC GCCTTTGACT TCTTCTTCTT CCCGTTCCTG
    TCGGCCGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974244.1
    CDS2..2653
    Translation

    Target ORF information:

    RefSeq Version XM_014118769.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris wolframin ER transmembrane glycoprotein (WFS1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118769.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGACGCCA GCACCCCCCC TACGAGCCCC TCTGGCCCAC AGCCTCCACC ACCGCAGCCC 
    CAGGCCCGCT CGCGGCTCAA TGCCACTGCC TCGGTGGAAC AGGACGAGAG GGGGGCGCCC
    CGTGCTCCGG GACCAGACGC TGCCAATGCG GCTGCCCCCG CTGCAGCCCG GGCCCCAAGT
    GCTCGGAGCC AGGAAAGGGC GGACAGAGCA GGGCCCATGA AGGCAGATGT GGAGGTGCCC
    TTTGGAGACG TGCTGGAGAG GGCCAAGGCA GGGGACGCCA AGGCGCAGAC GGAGGCGGGG
    AAGCACTACC TGCTCCTGGC GGGCACCGGG GACGAGGAGC TGAACAACTG CACGGCTGTC
    GACTGGCTCA TCCTCGCTGC CAAGCAGGGC CGGCGCGAGG CTGTCAAGCT CCTGCGCAGG
    TGCCTGGCCG ACAGGAAAGG CATCACTTTT GAGAATGAGC AGGAGGTGAA GCAGCTCTCC
    TCTGAGACCG ACCTGGGAGC GGGGCGGTGC GCAAGGCGGG CCCTGGTCAT GTACTGGGAA
    GCTCAACCCC CAAGAAGAAA GAAGCAGGTG GCCGTGTCCG AGCTGCTGGA GAACGTGGGT
    CAGGTCAACG AGCAGGAGGG AGCGGCACAG CCGGGCCCCA TCCCCAAGTC CCTCCAGAAG
    CAGAGGCGGG TGCTGGAGCG CTTGGTCAGC AGCGAATCCA AGAGCCACAT AGCGCCGGAC
    GACTTCGTGG AGATCACCAA GAAGTACGCC AAGGGCATCA TCCCCGCCAA CCTGTTCCTG
    CAGGACGACG ACGACGACGA GCTGGCCGGC AAGGGCCCCG AGGACCTGCC CCTGCGTCTG
    AAGATGGTCA AGTACCCGTT GCACGCTGTC ATGGAGATCA AGGAGTACCT GATCGATGTG
    GCCTCGCGGG CGGGCATGCA CTGGCTGTCC AGCATCGTGC CCACCCAGCA CATCAACGCC
    CTCATCTTCT TCTTCATCAT CAGCAACCTG ACCATCGACT TCTTCGCCTT CTTCATCCCG
    CTGGTCGTCT TCTACCTGTC GTTCGTCTCC ATGGTGATCT GCACCCTCAA GGTGTTCCAG
    GACAGCAGGG CGTGGGAGAG CTTCCGCACC CTCACGGGCC TGCTCCTGCG CTTCGAGCCC
    AACCTGGACG TGGAGCAGGC CGAGGTCAAC TTCGGCTGGA ACCACCTGGA GCCCTACGTG
    CACTTCCTGC TGTCTGTCTT CTTCGTCATC TTCTCCTTCC CCATCGCCAG CAAGGACTGC
    ATCCCCTGCT CTGAGCTGGC CGTCGTGTCC ATCTTCTTCA CCGCCACCAG CTACGTGAGC
    CTGAGCTCGT CTGCCGAGCC CTACACCCGG AGGGCCCTGG TGACCGAGGT GGCCGCCGGC
    CTACTGTCCC TCCTGCCCAC CCTGCCCTGC GGCTGGCAGC ACCTGAAGCT CCTGGGCCAG
    ACCTTCGTCA CCGTGCCCCT GGGCCACTTG GTCGTCCTGA ACGTCAGCGT CCCCTGCCTG
    CTGTACGTGT ACCTCCTGTA CCTCTTCTTC CGCATGGCGC AGCTGCGGGG CTTCAAGGGC
    ACCTACTGCT ACCTGGTGCC CTACCTGGTC TGCTTTATGT GGTGTGAGCT GTCCGTGGTC
    ATCCTGCTCG AGTCCACCGG CCTGGGGCTT GCCCGAGCCA CCGTGGGCTA CTTCCTCTTC
    CTGTTCGCGC TCCCCGTCCT GGTGGCCGGC CTGGCGCTCA TGGGCGTGCT GCAGCTTGCC
    CGCTGGTTCC TGTCGCTGGA GCTCACCAAG GTGGCCGTCA CTGCGCTGCT GTGCAGCGTC
    CCCCTGCTGT TCCGCTGGTG GAGCAAGGCC AGCTTCTCGC CGGTGGCCAT GGTCAAGTCC
    CTGACGCGGA GCTCCGTCGT CAAGCTCATC CTGGTGTGGA TCACGGCCAT CGTGCTCTTC
    TGCTGGCTGT ATGTGTACCG CTCGGAGGGC ATGAAGGTCT ACAACTCCAC GCTCACCTGG
    CAGCAGTACG GCTTCCTGTG CGGGCCCCGG GCCTGGAAGG AGACCAACAT GGCCCGCACC
    CAGATCCTGT GCAGCCACCT GGAAGGCCAC AGGGTCACAT GGACGGGCCG CTTCAAGTAC
    GTGCGGGTGA CGGAGATCGA CAACAGCGCC GAGGCGGCCA TCAACATGCT CCCGCTCTTC
    GTGGGCGACT GGATGCGCTG CCTGTACGGC GAGGCCTACC CGTCCTGCAG CCCCGGCAAT
    GCCTCCACGG CCGCGGAGGA GCTATGCCGC CTGAAGCTCC TGGCCAAGCA CCCCTGCCAC
    ATCCGCAAGT TCGACCGCTA CAAGTTCGAG ATCACGGTGG GCATGCCCTA CAGTGGCGCC
    AACGGCTCCC GCGGCCCAGA GGAGGACGAC GTCACCAAGG ACATCGTGCT GCGGGCCAGC
    GGCGAGTTCA AGGACGTGCT TCTCGGCCTG CGCCACGGCA GCCTCATCGA GTTCAGCACC
    GTCCTCGAGG GCCGCCTGGG CAGCAAGTGG CCCGTCTTCG AGCTCAAGGC CATCCGCTGC
    CTCAACTGCA TGGCACAGCT GTCCCCCGCC CGGCGCCACG TGAAGATAGA GCACGACTGG
    CGCAGCACCG TGCACGGCGC CGTCAAGTTC GCCTTTGACT TCTTCTTCTT CCCGTTCCTG
    TCGGCCGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39784
    Clone ID Related Accession (Same CDS sequence) XM_014118771.1 , XM_014118772.2 , XM_014118772.1 , XM_014118770.2 , XM_014118771.2 , XM_014118770.1 , XM_014118773.1
    Accession Version XM_014118771.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2436bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product wolframin isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726083.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGAAGGCAG ATGTGGAGGT GCCCTTTGGA GACGTGCTGG AGAGGGCCAA GGCAGGGGAC 
    GCCAAGGCGC AGACGGAGGC GGGGAAGCAC TACCTGCTCC TGGCGGGCAC CGGGGACGAG
    GAGCTGAACA ACTGCACGGC TGTCGACTGG CTCATCCTCG CTGCCAAGCA GGGCCGGCGC
    GAGGCTGTCA AGCTCCTGCG CAGGTGCCTG GCCGACAGGA AAGGCATCAC TTTTGAGAAT
    GAGCAGGAGG TGAAGCAGCT CTCCTCTGAG ACCGACCTGG GAGCGGGGCG GTGCGCAAGG
    CGGGCCCTGG TCATGTACTG GGAAGCTCAA CCCCCAAGAA GAAAGAAGCA GGTGGCCGTG
    TCCGAGCTGC TGGAGAACGT GGGTCAGGTC AACGAGCAGG AGGGAGCGGC ACAGCCGGGC
    CCCATCCCCA AGTCCCTCCA GAAGCAGAGG CGGGTGCTGG AGCGCTTGGT CAGCAGCGAA
    TCCAAGAGCC ACATAGCGCC GGACGACTTC GTGGAGATCA CCAAGAAGTA CGCCAAGGGC
    ATCATCCCCG CCAACCTGTT CCTGCAGGAC GACGACGACG ACGAGCTGGC CGGCAAGGGC
    CCCGAGGACC TGCCCCTGCG TCTGAAGATG GTCAAGTACC CGTTGCACGC TGTCATGGAG
    ATCAAGGAGT ACCTGATCGA TGTGGCCTCG CGGGCGGGCA TGCACTGGCT GTCCAGCATC
    GTGCCCACCC AGCACATCAA CGCCCTCATC TTCTTCTTCA TCATCAGCAA CCTGACCATC
    GACTTCTTCG CCTTCTTCAT CCCGCTGGTC GTCTTCTACC TGTCGTTCGT CTCCATGGTG
    ATCTGCACCC TCAAGGTGTT CCAGGACAGC AGGGCGTGGG AGAGCTTCCG CACCCTCACG
    GGCCTGCTCC TGCGCTTCGA GCCCAACCTG GACGTGGAGC AGGCCGAGGT CAACTTCGGC
    TGGAACCACC TGGAGCCCTA CGTGCACTTC CTGCTGTCTG TCTTCTTCGT CATCTTCTCC
    TTCCCCATCG CCAGCAAGGA CTGCATCCCC TGCTCTGAGC TGGCCGTCGT GTCCATCTTC
    TTCACCGCCA CCAGCTACGT GAGCCTGAGC TCGTCTGCCG AGCCCTACAC CCGGAGGGCC
    CTGGTGACCG AGGTGGCCGC CGGCCTACTG TCCCTCCTGC CCACCCTGCC CTGCGGCTGG
    CAGCACCTGA AGCTCCTGGG CCAGACCTTC GTCACCGTGC CCCTGGGCCA CTTGGTCGTC
    CTGAACGTCA GCGTCCCCTG CCTGCTGTAC GTGTACCTCC TGTACCTCTT CTTCCGCATG
    GCGCAGCTGC GGGGCTTCAA GGGCACCTAC TGCTACCTGG TGCCCTACCT GGTCTGCTTT
    ATGTGGTGTG AGCTGTCCGT GGTCATCCTG CTCGAGTCCA CCGGCCTGGG GCTTGCCCGA
    GCCACCGTGG GCTACTTCCT CTTCCTGTTC GCGCTCCCCG TCCTGGTGGC CGGCCTGGCG
    CTCATGGGCG TGCTGCAGCT TGCCCGCTGG TTCCTGTCGC TGGAGCTCAC CAAGGTGGCC
    GTCACTGCGC TGCTGTGCAG CGTCCCCCTG CTGTTCCGCT GGTGGAGCAA GGCCAGCTTC
    TCGCCGGTGG CCATGGTCAA GTCCCTGACG CGGAGCTCCG TCGTCAAGCT CATCCTGGTG
    TGGATCACGG CCATCGTGCT CTTCTGCTGG CTGTATGTGT ACCGCTCGGA GGGCATGAAG
    GTCTACAACT CCACGCTCAC CTGGCAGCAG TACGGCTTCC TGTGCGGGCC CCGGGCCTGG
    AAGGAGACCA ACATGGCCCG CACCCAGATC CTGTGCAGCC ACCTGGAAGG CCACAGGGTC
    ACATGGACGG GCCGCTTCAA GTACGTGCGG GTGACGGAGA TCGACAACAG CGCCGAGGCG
    GCCATCAACA TGCTCCCGCT CTTCGTGGGC GACTGGATGC GCTGCCTGTA CGGCGAGGCC
    TACCCGTCCT GCAGCCCCGG CAATGCCTCC ACGGCCGCGG AGGAGCTATG CCGCCTGAAG
    CTCCTGGCCA AGCACCCCTG CCACATCCGC AAGTTCGACC GCTACAAGTT CGAGATCACG
    GTGGGCATGC CCTACAGTGG CGCCAACGGC TCCCGCGGCC CAGAGGAGGA CGACGTCACC
    AAGGACATCG TGCTGCGGGC CAGCGGCGAG TTCAAGGACG TGCTTCTCGG CCTGCGCCAC
    GGCAGCCTCA TCGAGTTCAG CACCGTCCTC GAGGGCCGCC TGGGCAGCAA GTGGCCCGTC
    TTCGAGCTCA AGGCCATCCG CTGCCTCAAC TGCATGGCAC AGCTGTCCCC CGCCCGGCGC
    CACGTGAAGA TAGAGCACGA CTGGCGCAGC ACCGTGCACG GCGCCGTCAA GTTCGCCTTT
    GACTTCTTCT TCTTCCCGTT CCTGTCGGCC GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974246.1
    CDS1767..4202
    Translation

    Target ORF information:

    RefSeq Version XM_014118771.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Wolfram syndrome 1 (wolframin) (WFS1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118771.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGAAGGCAG ATGTGGAGGT GCCCTTTGGA GACGTGCTGG AGAGGGCCAA GGCAGGGGAC 
    GCCAAGGCGC AGACGGAGGC GGGGAAGCAC TACCTGCTCC TGGCGGGCAC CGGGGACGAG
    GAGCTGAACA ACTGCACGGC TGTCGACTGG CTCATCCTCG CTGCCAAGCA GGGCCGGCGC
    GAGGCTGTCA AGCTCCTGCG CAGGTGCCTG GCCGACAGGA AAGGCATCAC TTTTGAGAAT
    GAGCAGGAGG TGAAGCAGCT CTCCTCTGAG ACCGACCTGG GAGCGGGGCG GTGCGCAAGG
    CGGGCCCTGG TCATGTACTG GGAAGCTCAA CCCCCAAGAA GAAAGAAGCA GGTGGCCGTG
    TCCGAGCTGC TGGAGAACGT GGGTCAGGTC AACGAGCAGG AGGGAGCGGC ACAGCCGGGC
    CCCATCCCCA AGTCCCTCCA GAAGCAGAGG CGGGTGCTGG AGCGCTTGGT CAGCAGCGAA
    TCCAAGAGCC ACATAGCGCC GGACGACTTC GTGGAGATCA CCAAGAAGTA CGCCAAGGGC
    ATCATCCCCG CCAACCTGTT CCTGCAGGAC GACGACGACG ACGAGCTGGC CGGCAAGGGC
    CCCGAGGACC TGCCCCTGCG TCTGAAGATG GTCAAGTACC CGTTGCACGC TGTCATGGAG
    ATCAAGGAGT ACCTGATCGA TGTGGCCTCG CGGGCGGGCA TGCACTGGCT GTCCAGCATC
    GTGCCCACCC AGCACATCAA CGCCCTCATC TTCTTCTTCA TCATCAGCAA CCTGACCATC
    GACTTCTTCG CCTTCTTCAT CCCGCTGGTC GTCTTCTACC TGTCGTTCGT CTCCATGGTG
    ATCTGCACCC TCAAGGTGTT CCAGGACAGC AGGGCGTGGG AGAGCTTCCG CACCCTCACG
    GGCCTGCTCC TGCGCTTCGA GCCCAACCTG GACGTGGAGC AGGCCGAGGT CAACTTCGGC
    TGGAACCACC TGGAGCCCTA CGTGCACTTC CTGCTGTCTG TCTTCTTCGT CATCTTCTCC
    TTCCCCATCG CCAGCAAGGA CTGCATCCCC TGCTCTGAGC TGGCCGTCGT GTCCATCTTC
    TTCACCGCCA CCAGCTACGT GAGCCTGAGC TCGTCTGCCG AGCCCTACAC CCGGAGGGCC
    CTGGTGACCG AGGTGGCCGC CGGCCTACTG TCCCTCCTGC CCACCCTGCC CTGCGGCTGG
    CAGCACCTGA AGCTCCTGGG CCAGACCTTC GTCACCGTGC CCCTGGGCCA CTTGGTCGTC
    CTGAACGTCA GCGTCCCCTG CCTGCTGTAC GTGTACCTCC TGTACCTCTT CTTCCGCATG
    GCGCAGCTGC GGGGCTTCAA GGGCACCTAC TGCTACCTGG TGCCCTACCT GGTCTGCTTT
    ATGTGGTGTG AGCTGTCCGT GGTCATCCTG CTCGAGTCCA CCGGCCTGGG GCTTGCCCGA
    GCCACCGTGG GCTACTTCCT CTTCCTGTTC GCGCTCCCCG TCCTGGTGGC CGGCCTGGCG
    CTCATGGGCG TGCTGCAGCT TGCCCGCTGG TTCCTGTCGC TGGAGCTCAC CAAGGTGGCC
    GTCACTGCGC TGCTGTGCAG CGTCCCCCTG CTGTTCCGCT GGTGGAGCAA GGCCAGCTTC
    TCGCCGGTGG CCATGGTCAA GTCCCTGACG CGGAGCTCCG TCGTCAAGCT CATCCTGGTG
    TGGATCACGG CCATCGTGCT CTTCTGCTGG CTGTATGTGT ACCGCTCGGA GGGCATGAAG
    GTCTACAACT CCACGCTCAC CTGGCAGCAG TACGGCTTCC TGTGCGGGCC CCGGGCCTGG
    AAGGAGACCA ACATGGCCCG CACCCAGATC CTGTGCAGCC ACCTGGAAGG CCACAGGGTC
    ACATGGACGG GCCGCTTCAA GTACGTGCGG GTGACGGAGA TCGACAACAG CGCCGAGGCG
    GCCATCAACA TGCTCCCGCT CTTCGTGGGC GACTGGATGC GCTGCCTGTA CGGCGAGGCC
    TACCCGTCCT GCAGCCCCGG CAATGCCTCC ACGGCCGCGG AGGAGCTATG CCGCCTGAAG
    CTCCTGGCCA AGCACCCCTG CCACATCCGC AAGTTCGACC GCTACAAGTT CGAGATCACG
    GTGGGCATGC CCTACAGTGG CGCCAACGGC TCCCGCGGCC CAGAGGAGGA CGACGTCACC
    AAGGACATCG TGCTGCGGGC CAGCGGCGAG TTCAAGGACG TGCTTCTCGG CCTGCGCCAC
    GGCAGCCTCA TCGAGTTCAG CACCGTCCTC GAGGGCCGCC TGGGCAGCAA GTGGCCCGTC
    TTCGAGCTCA AGGCCATCCG CTGCCTCAAC TGCATGGCAC AGCTGTCCCC CGCCCGGCGC
    CACGTGAAGA TAGAGCACGA CTGGCGCAGC ACCGTGCACG GCGCCGTCAA GTTCGCCTTT
    GACTTCTTCT TCTTCCCGTT CCTGTCGGCC GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39784
    Clone ID Related Accession (Same CDS sequence) XM_014118771.1 , XM_014118772.2 , XM_014118772.1 , XM_014118770.2 , XM_014118771.2 , XM_014118770.1 , XM_014118773.1
    Accession Version XM_014118772.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2436bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product wolframin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014118772.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGAAGGCAG ATGTGGAGGT GCCCTTTGGA GACGTGCTGG AGAGGGCCAA GGCAGGGGAC 
    GCCAAGGCGC AGACGGAGGC GGGGAAGCAC TACCTGCTCC TGGCGGGCAC CGGGGACGAG
    GAGCTGAACA ACTGCACGGC TGTCGACTGG CTCATCCTCG CTGCCAAGCA GGGCCGGCGC
    GAGGCTGTCA AGCTCCTGCG CAGGTGCCTG GCCGACAGGA AAGGCATCAC TTTTGAGAAT
    GAGCAGGAGG TGAAGCAGCT CTCCTCTGAG ACCGACCTGG GAGCGGGGCG GTGCGCAAGG
    CGGGCCCTGG TCATGTACTG GGAAGCTCAA CCCCCAAGAA GAAAGAAGCA GGTGGCCGTG
    TCCGAGCTGC TGGAGAACGT GGGTCAGGTC AACGAGCAGG AGGGAGCGGC ACAGCCGGGC
    CCCATCCCCA AGTCCCTCCA GAAGCAGAGG CGGGTGCTGG AGCGCTTGGT CAGCAGCGAA
    TCCAAGAGCC ACATAGCGCC GGACGACTTC GTGGAGATCA CCAAGAAGTA CGCCAAGGGC
    ATCATCCCCG CCAACCTGTT CCTGCAGGAC GACGACGACG ACGAGCTGGC CGGCAAGGGC
    CCCGAGGACC TGCCCCTGCG TCTGAAGATG GTCAAGTACC CGTTGCACGC TGTCATGGAG
    ATCAAGGAGT ACCTGATCGA TGTGGCCTCG CGGGCGGGCA TGCACTGGCT GTCCAGCATC
    GTGCCCACCC AGCACATCAA CGCCCTCATC TTCTTCTTCA TCATCAGCAA CCTGACCATC
    GACTTCTTCG CCTTCTTCAT CCCGCTGGTC GTCTTCTACC TGTCGTTCGT CTCCATGGTG
    ATCTGCACCC TCAAGGTGTT CCAGGACAGC AGGGCGTGGG AGAGCTTCCG CACCCTCACG
    GGCCTGCTCC TGCGCTTCGA GCCCAACCTG GACGTGGAGC AGGCCGAGGT CAACTTCGGC
    TGGAACCACC TGGAGCCCTA CGTGCACTTC CTGCTGTCTG TCTTCTTCGT CATCTTCTCC
    TTCCCCATCG CCAGCAAGGA CTGCATCCCC TGCTCTGAGC TGGCCGTCGT GTCCATCTTC
    TTCACCGCCA CCAGCTACGT GAGCCTGAGC TCGTCTGCCG AGCCCTACAC CCGGAGGGCC
    CTGGTGACCG AGGTGGCCGC CGGCCTACTG TCCCTCCTGC CCACCCTGCC CTGCGGCTGG
    CAGCACCTGA AGCTCCTGGG CCAGACCTTC GTCACCGTGC CCCTGGGCCA CTTGGTCGTC
    CTGAACGTCA GCGTCCCCTG CCTGCTGTAC GTGTACCTCC TGTACCTCTT CTTCCGCATG
    GCGCAGCTGC GGGGCTTCAA GGGCACCTAC TGCTACCTGG TGCCCTACCT GGTCTGCTTT
    ATGTGGTGTG AGCTGTCCGT GGTCATCCTG CTCGAGTCCA CCGGCCTGGG GCTTGCCCGA
    GCCACCGTGG GCTACTTCCT CTTCCTGTTC GCGCTCCCCG TCCTGGTGGC CGGCCTGGCG
    CTCATGGGCG TGCTGCAGCT TGCCCGCTGG TTCCTGTCGC TGGAGCTCAC CAAGGTGGCC
    GTCACTGCGC TGCTGTGCAG CGTCCCCCTG CTGTTCCGCT GGTGGAGCAA GGCCAGCTTC
    TCGCCGGTGG CCATGGTCAA GTCCCTGACG CGGAGCTCCG TCGTCAAGCT CATCCTGGTG
    TGGATCACGG CCATCGTGCT CTTCTGCTGG CTGTATGTGT ACCGCTCGGA GGGCATGAAG
    GTCTACAACT CCACGCTCAC CTGGCAGCAG TACGGCTTCC TGTGCGGGCC CCGGGCCTGG
    AAGGAGACCA ACATGGCCCG CACCCAGATC CTGTGCAGCC ACCTGGAAGG CCACAGGGTC
    ACATGGACGG GCCGCTTCAA GTACGTGCGG GTGACGGAGA TCGACAACAG CGCCGAGGCG
    GCCATCAACA TGCTCCCGCT CTTCGTGGGC GACTGGATGC GCTGCCTGTA CGGCGAGGCC
    TACCCGTCCT GCAGCCCCGG CAATGCCTCC ACGGCCGCGG AGGAGCTATG CCGCCTGAAG
    CTCCTGGCCA AGCACCCCTG CCACATCCGC AAGTTCGACC GCTACAAGTT CGAGATCACG
    GTGGGCATGC CCTACAGTGG CGCCAACGGC TCCCGCGGCC CAGAGGAGGA CGACGTCACC
    AAGGACATCG TGCTGCGGGC CAGCGGCGAG TTCAAGGACG TGCTTCTCGG CCTGCGCCAC
    GGCAGCCTCA TCGAGTTCAG CACCGTCCTC GAGGGCCGCC TGGGCAGCAA GTGGCCCGTC
    TTCGAGCTCA AGGCCATCCG CTGCCTCAAC TGCATGGCAC AGCTGTCCCC CGCCCGGCGC
    CACGTGAAGA TAGAGCACGA CTGGCGCAGC ACCGTGCACG GCGCCGTCAA GTTCGCCTTT
    GACTTCTTCT TCTTCCCGTT CCTGTCGGCC GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974247.1
    CDS546..2981
    Translation

    Target ORF information:

    RefSeq Version XM_014118772.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris wolframin ER transmembrane glycoprotein (WFS1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118772.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGAAGGCAG ATGTGGAGGT GCCCTTTGGA GACGTGCTGG AGAGGGCCAA GGCAGGGGAC 
    GCCAAGGCGC AGACGGAGGC GGGGAAGCAC TACCTGCTCC TGGCGGGCAC CGGGGACGAG
    GAGCTGAACA ACTGCACGGC TGTCGACTGG CTCATCCTCG CTGCCAAGCA GGGCCGGCGC
    GAGGCTGTCA AGCTCCTGCG CAGGTGCCTG GCCGACAGGA AAGGCATCAC TTTTGAGAAT
    GAGCAGGAGG TGAAGCAGCT CTCCTCTGAG ACCGACCTGG GAGCGGGGCG GTGCGCAAGG
    CGGGCCCTGG TCATGTACTG GGAAGCTCAA CCCCCAAGAA GAAAGAAGCA GGTGGCCGTG
    TCCGAGCTGC TGGAGAACGT GGGTCAGGTC AACGAGCAGG AGGGAGCGGC ACAGCCGGGC
    CCCATCCCCA AGTCCCTCCA GAAGCAGAGG CGGGTGCTGG AGCGCTTGGT CAGCAGCGAA
    TCCAAGAGCC ACATAGCGCC GGACGACTTC GTGGAGATCA CCAAGAAGTA CGCCAAGGGC
    ATCATCCCCG CCAACCTGTT CCTGCAGGAC GACGACGACG ACGAGCTGGC CGGCAAGGGC
    CCCGAGGACC TGCCCCTGCG TCTGAAGATG GTCAAGTACC CGTTGCACGC TGTCATGGAG
    ATCAAGGAGT ACCTGATCGA TGTGGCCTCG CGGGCGGGCA TGCACTGGCT GTCCAGCATC
    GTGCCCACCC AGCACATCAA CGCCCTCATC TTCTTCTTCA TCATCAGCAA CCTGACCATC
    GACTTCTTCG CCTTCTTCAT CCCGCTGGTC GTCTTCTACC TGTCGTTCGT CTCCATGGTG
    ATCTGCACCC TCAAGGTGTT CCAGGACAGC AGGGCGTGGG AGAGCTTCCG CACCCTCACG
    GGCCTGCTCC TGCGCTTCGA GCCCAACCTG GACGTGGAGC AGGCCGAGGT CAACTTCGGC
    TGGAACCACC TGGAGCCCTA CGTGCACTTC CTGCTGTCTG TCTTCTTCGT CATCTTCTCC
    TTCCCCATCG CCAGCAAGGA CTGCATCCCC TGCTCTGAGC TGGCCGTCGT GTCCATCTTC
    TTCACCGCCA CCAGCTACGT GAGCCTGAGC TCGTCTGCCG AGCCCTACAC CCGGAGGGCC
    CTGGTGACCG AGGTGGCCGC CGGCCTACTG TCCCTCCTGC CCACCCTGCC CTGCGGCTGG
    CAGCACCTGA AGCTCCTGGG CCAGACCTTC GTCACCGTGC CCCTGGGCCA CTTGGTCGTC
    CTGAACGTCA GCGTCCCCTG CCTGCTGTAC GTGTACCTCC TGTACCTCTT CTTCCGCATG
    GCGCAGCTGC GGGGCTTCAA GGGCACCTAC TGCTACCTGG TGCCCTACCT GGTCTGCTTT
    ATGTGGTGTG AGCTGTCCGT GGTCATCCTG CTCGAGTCCA CCGGCCTGGG GCTTGCCCGA
    GCCACCGTGG GCTACTTCCT CTTCCTGTTC GCGCTCCCCG TCCTGGTGGC CGGCCTGGCG
    CTCATGGGCG TGCTGCAGCT TGCCCGCTGG TTCCTGTCGC TGGAGCTCAC CAAGGTGGCC
    GTCACTGCGC TGCTGTGCAG CGTCCCCCTG CTGTTCCGCT GGTGGAGCAA GGCCAGCTTC
    TCGCCGGTGG CCATGGTCAA GTCCCTGACG CGGAGCTCCG TCGTCAAGCT CATCCTGGTG
    TGGATCACGG CCATCGTGCT CTTCTGCTGG CTGTATGTGT ACCGCTCGGA GGGCATGAAG
    GTCTACAACT CCACGCTCAC CTGGCAGCAG TACGGCTTCC TGTGCGGGCC CCGGGCCTGG
    AAGGAGACCA ACATGGCCCG CACCCAGATC CTGTGCAGCC ACCTGGAAGG CCACAGGGTC
    ACATGGACGG GCCGCTTCAA GTACGTGCGG GTGACGGAGA TCGACAACAG CGCCGAGGCG
    GCCATCAACA TGCTCCCGCT CTTCGTGGGC GACTGGATGC GCTGCCTGTA CGGCGAGGCC
    TACCCGTCCT GCAGCCCCGG CAATGCCTCC ACGGCCGCGG AGGAGCTATG CCGCCTGAAG
    CTCCTGGCCA AGCACCCCTG CCACATCCGC AAGTTCGACC GCTACAAGTT CGAGATCACG
    GTGGGCATGC CCTACAGTGG CGCCAACGGC TCCCGCGGCC CAGAGGAGGA CGACGTCACC
    AAGGACATCG TGCTGCGGGC CAGCGGCGAG TTCAAGGACG TGCTTCTCGG CCTGCGCCAC
    GGCAGCCTCA TCGAGTTCAG CACCGTCCTC GAGGGCCGCC TGGGCAGCAA GTGGCCCGTC
    TTCGAGCTCA AGGCCATCCG CTGCCTCAAC TGCATGGCAC AGCTGTCCCC CGCCCGGCGC
    CACGTGAAGA TAGAGCACGA CTGGCGCAGC ACCGTGCACG GCGCCGTCAA GTTCGCCTTT
    GACTTCTTCT TCTTCCCGTT CCTGTCGGCC GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39784
    Clone ID Related Accession (Same CDS sequence) XM_014118771.1 , XM_014118772.2 , XM_014118772.1 , XM_014118770.2 , XM_014118771.2 , XM_014118770.1 , XM_014118773.1
    Accession Version XM_014118772.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2436bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product wolframin isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726083.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGAAGGCAG ATGTGGAGGT GCCCTTTGGA GACGTGCTGG AGAGGGCCAA GGCAGGGGAC 
    GCCAAGGCGC AGACGGAGGC GGGGAAGCAC TACCTGCTCC TGGCGGGCAC CGGGGACGAG
    GAGCTGAACA ACTGCACGGC TGTCGACTGG CTCATCCTCG CTGCCAAGCA GGGCCGGCGC
    GAGGCTGTCA AGCTCCTGCG CAGGTGCCTG GCCGACAGGA AAGGCATCAC TTTTGAGAAT
    GAGCAGGAGG TGAAGCAGCT CTCCTCTGAG ACCGACCTGG GAGCGGGGCG GTGCGCAAGG
    CGGGCCCTGG TCATGTACTG GGAAGCTCAA CCCCCAAGAA GAAAGAAGCA GGTGGCCGTG
    TCCGAGCTGC TGGAGAACGT GGGTCAGGTC AACGAGCAGG AGGGAGCGGC ACAGCCGGGC
    CCCATCCCCA AGTCCCTCCA GAAGCAGAGG CGGGTGCTGG AGCGCTTGGT CAGCAGCGAA
    TCCAAGAGCC ACATAGCGCC GGACGACTTC GTGGAGATCA CCAAGAAGTA CGCCAAGGGC
    ATCATCCCCG CCAACCTGTT CCTGCAGGAC GACGACGACG ACGAGCTGGC CGGCAAGGGC
    CCCGAGGACC TGCCCCTGCG TCTGAAGATG GTCAAGTACC CGTTGCACGC TGTCATGGAG
    ATCAAGGAGT ACCTGATCGA TGTGGCCTCG CGGGCGGGCA TGCACTGGCT GTCCAGCATC
    GTGCCCACCC AGCACATCAA CGCCCTCATC TTCTTCTTCA TCATCAGCAA CCTGACCATC
    GACTTCTTCG CCTTCTTCAT CCCGCTGGTC GTCTTCTACC TGTCGTTCGT CTCCATGGTG
    ATCTGCACCC TCAAGGTGTT CCAGGACAGC AGGGCGTGGG AGAGCTTCCG CACCCTCACG
    GGCCTGCTCC TGCGCTTCGA GCCCAACCTG GACGTGGAGC AGGCCGAGGT CAACTTCGGC
    TGGAACCACC TGGAGCCCTA CGTGCACTTC CTGCTGTCTG TCTTCTTCGT CATCTTCTCC
    TTCCCCATCG CCAGCAAGGA CTGCATCCCC TGCTCTGAGC TGGCCGTCGT GTCCATCTTC
    TTCACCGCCA CCAGCTACGT GAGCCTGAGC TCGTCTGCCG AGCCCTACAC CCGGAGGGCC
    CTGGTGACCG AGGTGGCCGC CGGCCTACTG TCCCTCCTGC CCACCCTGCC CTGCGGCTGG
    CAGCACCTGA AGCTCCTGGG CCAGACCTTC GTCACCGTGC CCCTGGGCCA CTTGGTCGTC
    CTGAACGTCA GCGTCCCCTG CCTGCTGTAC GTGTACCTCC TGTACCTCTT CTTCCGCATG
    GCGCAGCTGC GGGGCTTCAA GGGCACCTAC TGCTACCTGG TGCCCTACCT GGTCTGCTTT
    ATGTGGTGTG AGCTGTCCGT GGTCATCCTG CTCGAGTCCA CCGGCCTGGG GCTTGCCCGA
    GCCACCGTGG GCTACTTCCT CTTCCTGTTC GCGCTCCCCG TCCTGGTGGC CGGCCTGGCG
    CTCATGGGCG TGCTGCAGCT TGCCCGCTGG TTCCTGTCGC TGGAGCTCAC CAAGGTGGCC
    GTCACTGCGC TGCTGTGCAG CGTCCCCCTG CTGTTCCGCT GGTGGAGCAA GGCCAGCTTC
    TCGCCGGTGG CCATGGTCAA GTCCCTGACG CGGAGCTCCG TCGTCAAGCT CATCCTGGTG
    TGGATCACGG CCATCGTGCT CTTCTGCTGG CTGTATGTGT ACCGCTCGGA GGGCATGAAG
    GTCTACAACT CCACGCTCAC CTGGCAGCAG TACGGCTTCC TGTGCGGGCC CCGGGCCTGG
    AAGGAGACCA ACATGGCCCG CACCCAGATC CTGTGCAGCC ACCTGGAAGG CCACAGGGTC
    ACATGGACGG GCCGCTTCAA GTACGTGCGG GTGACGGAGA TCGACAACAG CGCCGAGGCG
    GCCATCAACA TGCTCCCGCT CTTCGTGGGC GACTGGATGC GCTGCCTGTA CGGCGAGGCC
    TACCCGTCCT GCAGCCCCGG CAATGCCTCC ACGGCCGCGG AGGAGCTATG CCGCCTGAAG
    CTCCTGGCCA AGCACCCCTG CCACATCCGC AAGTTCGACC GCTACAAGTT CGAGATCACG
    GTGGGCATGC CCTACAGTGG CGCCAACGGC TCCCGCGGCC CAGAGGAGGA CGACGTCACC
    AAGGACATCG TGCTGCGGGC CAGCGGCGAG TTCAAGGACG TGCTTCTCGG CCTGCGCCAC
    GGCAGCCTCA TCGAGTTCAG CACCGTCCTC GAGGGCCGCC TGGGCAGCAA GTGGCCCGTC
    TTCGAGCTCA AGGCCATCCG CTGCCTCAAC TGCATGGCAC AGCTGTCCCC CGCCCGGCGC
    CACGTGAAGA TAGAGCACGA CTGGCGCAGC ACCGTGCACG GCGCCGTCAA GTTCGCCTTT
    GACTTCTTCT TCTTCCCGTT CCTGTCGGCC GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974247.1
    CDS551..2986
    Translation

    Target ORF information:

    RefSeq Version XM_014118772.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Wolfram syndrome 1 (wolframin) (WFS1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118772.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGAAGGCAG ATGTGGAGGT GCCCTTTGGA GACGTGCTGG AGAGGGCCAA GGCAGGGGAC 
    GCCAAGGCGC AGACGGAGGC GGGGAAGCAC TACCTGCTCC TGGCGGGCAC CGGGGACGAG
    GAGCTGAACA ACTGCACGGC TGTCGACTGG CTCATCCTCG CTGCCAAGCA GGGCCGGCGC
    GAGGCTGTCA AGCTCCTGCG CAGGTGCCTG GCCGACAGGA AAGGCATCAC TTTTGAGAAT
    GAGCAGGAGG TGAAGCAGCT CTCCTCTGAG ACCGACCTGG GAGCGGGGCG GTGCGCAAGG
    CGGGCCCTGG TCATGTACTG GGAAGCTCAA CCCCCAAGAA GAAAGAAGCA GGTGGCCGTG
    TCCGAGCTGC TGGAGAACGT GGGTCAGGTC AACGAGCAGG AGGGAGCGGC ACAGCCGGGC
    CCCATCCCCA AGTCCCTCCA GAAGCAGAGG CGGGTGCTGG AGCGCTTGGT CAGCAGCGAA
    TCCAAGAGCC ACATAGCGCC GGACGACTTC GTGGAGATCA CCAAGAAGTA CGCCAAGGGC
    ATCATCCCCG CCAACCTGTT CCTGCAGGAC GACGACGACG ACGAGCTGGC CGGCAAGGGC
    CCCGAGGACC TGCCCCTGCG TCTGAAGATG GTCAAGTACC CGTTGCACGC TGTCATGGAG
    ATCAAGGAGT ACCTGATCGA TGTGGCCTCG CGGGCGGGCA TGCACTGGCT GTCCAGCATC
    GTGCCCACCC AGCACATCAA CGCCCTCATC TTCTTCTTCA TCATCAGCAA CCTGACCATC
    GACTTCTTCG CCTTCTTCAT CCCGCTGGTC GTCTTCTACC TGTCGTTCGT CTCCATGGTG
    ATCTGCACCC TCAAGGTGTT CCAGGACAGC AGGGCGTGGG AGAGCTTCCG CACCCTCACG
    GGCCTGCTCC TGCGCTTCGA GCCCAACCTG GACGTGGAGC AGGCCGAGGT CAACTTCGGC
    TGGAACCACC TGGAGCCCTA CGTGCACTTC CTGCTGTCTG TCTTCTTCGT CATCTTCTCC
    TTCCCCATCG CCAGCAAGGA CTGCATCCCC TGCTCTGAGC TGGCCGTCGT GTCCATCTTC
    TTCACCGCCA CCAGCTACGT GAGCCTGAGC TCGTCTGCCG AGCCCTACAC CCGGAGGGCC
    CTGGTGACCG AGGTGGCCGC CGGCCTACTG TCCCTCCTGC CCACCCTGCC CTGCGGCTGG
    CAGCACCTGA AGCTCCTGGG CCAGACCTTC GTCACCGTGC CCCTGGGCCA CTTGGTCGTC
    CTGAACGTCA GCGTCCCCTG CCTGCTGTAC GTGTACCTCC TGTACCTCTT CTTCCGCATG
    GCGCAGCTGC GGGGCTTCAA GGGCACCTAC TGCTACCTGG TGCCCTACCT GGTCTGCTTT
    ATGTGGTGTG AGCTGTCCGT GGTCATCCTG CTCGAGTCCA CCGGCCTGGG GCTTGCCCGA
    GCCACCGTGG GCTACTTCCT CTTCCTGTTC GCGCTCCCCG TCCTGGTGGC CGGCCTGGCG
    CTCATGGGCG TGCTGCAGCT TGCCCGCTGG TTCCTGTCGC TGGAGCTCAC CAAGGTGGCC
    GTCACTGCGC TGCTGTGCAG CGTCCCCCTG CTGTTCCGCT GGTGGAGCAA GGCCAGCTTC
    TCGCCGGTGG CCATGGTCAA GTCCCTGACG CGGAGCTCCG TCGTCAAGCT CATCCTGGTG
    TGGATCACGG CCATCGTGCT CTTCTGCTGG CTGTATGTGT ACCGCTCGGA GGGCATGAAG
    GTCTACAACT CCACGCTCAC CTGGCAGCAG TACGGCTTCC TGTGCGGGCC CCGGGCCTGG
    AAGGAGACCA ACATGGCCCG CACCCAGATC CTGTGCAGCC ACCTGGAAGG CCACAGGGTC
    ACATGGACGG GCCGCTTCAA GTACGTGCGG GTGACGGAGA TCGACAACAG CGCCGAGGCG
    GCCATCAACA TGCTCCCGCT CTTCGTGGGC GACTGGATGC GCTGCCTGTA CGGCGAGGCC
    TACCCGTCCT GCAGCCCCGG CAATGCCTCC ACGGCCGCGG AGGAGCTATG CCGCCTGAAG
    CTCCTGGCCA AGCACCCCTG CCACATCCGC AAGTTCGACC GCTACAAGTT CGAGATCACG
    GTGGGCATGC CCTACAGTGG CGCCAACGGC TCCCGCGGCC CAGAGGAGGA CGACGTCACC
    AAGGACATCG TGCTGCGGGC CAGCGGCGAG TTCAAGGACG TGCTTCTCGG CCTGCGCCAC
    GGCAGCCTCA TCGAGTTCAG CACCGTCCTC GAGGGCCGCC TGGGCAGCAA GTGGCCCGTC
    TTCGAGCTCA AGGCCATCCG CTGCCTCAAC TGCATGGCAC AGCTGTCCCC CGCCCGGCGC
    CACGTGAAGA TAGAGCACGA CTGGCGCAGC ACCGTGCACG GCGCCGTCAA GTTCGCCTTT
    GACTTCTTCT TCTTCCCGTT CCTGTCGGCC GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39784
    Clone ID Related Accession (Same CDS sequence) XM_014118771.1 , XM_014118772.2 , XM_014118772.1 , XM_014118770.2 , XM_014118771.2 , XM_014118770.1 , XM_014118773.1
    Accession Version XM_014118770.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2436bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product wolframin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014118770.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGAAGGCAG ATGTGGAGGT GCCCTTTGGA GACGTGCTGG AGAGGGCCAA GGCAGGGGAC 
    GCCAAGGCGC AGACGGAGGC GGGGAAGCAC TACCTGCTCC TGGCGGGCAC CGGGGACGAG
    GAGCTGAACA ACTGCACGGC TGTCGACTGG CTCATCCTCG CTGCCAAGCA GGGCCGGCGC
    GAGGCTGTCA AGCTCCTGCG CAGGTGCCTG GCCGACAGGA AAGGCATCAC TTTTGAGAAT
    GAGCAGGAGG TGAAGCAGCT CTCCTCTGAG ACCGACCTGG GAGCGGGGCG GTGCGCAAGG
    CGGGCCCTGG TCATGTACTG GGAAGCTCAA CCCCCAAGAA GAAAGAAGCA GGTGGCCGTG
    TCCGAGCTGC TGGAGAACGT GGGTCAGGTC AACGAGCAGG AGGGAGCGGC ACAGCCGGGC
    CCCATCCCCA AGTCCCTCCA GAAGCAGAGG CGGGTGCTGG AGCGCTTGGT CAGCAGCGAA
    TCCAAGAGCC ACATAGCGCC GGACGACTTC GTGGAGATCA CCAAGAAGTA CGCCAAGGGC
    ATCATCCCCG CCAACCTGTT CCTGCAGGAC GACGACGACG ACGAGCTGGC CGGCAAGGGC
    CCCGAGGACC TGCCCCTGCG TCTGAAGATG GTCAAGTACC CGTTGCACGC TGTCATGGAG
    ATCAAGGAGT ACCTGATCGA TGTGGCCTCG CGGGCGGGCA TGCACTGGCT GTCCAGCATC
    GTGCCCACCC AGCACATCAA CGCCCTCATC TTCTTCTTCA TCATCAGCAA CCTGACCATC
    GACTTCTTCG CCTTCTTCAT CCCGCTGGTC GTCTTCTACC TGTCGTTCGT CTCCATGGTG
    ATCTGCACCC TCAAGGTGTT CCAGGACAGC AGGGCGTGGG AGAGCTTCCG CACCCTCACG
    GGCCTGCTCC TGCGCTTCGA GCCCAACCTG GACGTGGAGC AGGCCGAGGT CAACTTCGGC
    TGGAACCACC TGGAGCCCTA CGTGCACTTC CTGCTGTCTG TCTTCTTCGT CATCTTCTCC
    TTCCCCATCG CCAGCAAGGA CTGCATCCCC TGCTCTGAGC TGGCCGTCGT GTCCATCTTC
    TTCACCGCCA CCAGCTACGT GAGCCTGAGC TCGTCTGCCG AGCCCTACAC CCGGAGGGCC
    CTGGTGACCG AGGTGGCCGC CGGCCTACTG TCCCTCCTGC CCACCCTGCC CTGCGGCTGG
    CAGCACCTGA AGCTCCTGGG CCAGACCTTC GTCACCGTGC CCCTGGGCCA CTTGGTCGTC
    CTGAACGTCA GCGTCCCCTG CCTGCTGTAC GTGTACCTCC TGTACCTCTT CTTCCGCATG
    GCGCAGCTGC GGGGCTTCAA GGGCACCTAC TGCTACCTGG TGCCCTACCT GGTCTGCTTT
    ATGTGGTGTG AGCTGTCCGT GGTCATCCTG CTCGAGTCCA CCGGCCTGGG GCTTGCCCGA
    GCCACCGTGG GCTACTTCCT CTTCCTGTTC GCGCTCCCCG TCCTGGTGGC CGGCCTGGCG
    CTCATGGGCG TGCTGCAGCT TGCCCGCTGG TTCCTGTCGC TGGAGCTCAC CAAGGTGGCC
    GTCACTGCGC TGCTGTGCAG CGTCCCCCTG CTGTTCCGCT GGTGGAGCAA GGCCAGCTTC
    TCGCCGGTGG CCATGGTCAA GTCCCTGACG CGGAGCTCCG TCGTCAAGCT CATCCTGGTG
    TGGATCACGG CCATCGTGCT CTTCTGCTGG CTGTATGTGT ACCGCTCGGA GGGCATGAAG
    GTCTACAACT CCACGCTCAC CTGGCAGCAG TACGGCTTCC TGTGCGGGCC CCGGGCCTGG
    AAGGAGACCA ACATGGCCCG CACCCAGATC CTGTGCAGCC ACCTGGAAGG CCACAGGGTC
    ACATGGACGG GCCGCTTCAA GTACGTGCGG GTGACGGAGA TCGACAACAG CGCCGAGGCG
    GCCATCAACA TGCTCCCGCT CTTCGTGGGC GACTGGATGC GCTGCCTGTA CGGCGAGGCC
    TACCCGTCCT GCAGCCCCGG CAATGCCTCC ACGGCCGCGG AGGAGCTATG CCGCCTGAAG
    CTCCTGGCCA AGCACCCCTG CCACATCCGC AAGTTCGACC GCTACAAGTT CGAGATCACG
    GTGGGCATGC CCTACAGTGG CGCCAACGGC TCCCGCGGCC CAGAGGAGGA CGACGTCACC
    AAGGACATCG TGCTGCGGGC CAGCGGCGAG TTCAAGGACG TGCTTCTCGG CCTGCGCCAC
    GGCAGCCTCA TCGAGTTCAG CACCGTCCTC GAGGGCCGCC TGGGCAGCAA GTGGCCCGTC
    TTCGAGCTCA AGGCCATCCG CTGCCTCAAC TGCATGGCAC AGCTGTCCCC CGCCCGGCGC
    CACGTGAAGA TAGAGCACGA CTGGCGCAGC ACCGTGCACG GCGCCGTCAA GTTCGCCTTT
    GACTTCTTCT TCTTCCCGTT CCTGTCGGCC GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974245.1
    CDS1844..4279
    Translation

    Target ORF information:

    RefSeq Version XM_014118770.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris wolframin ER transmembrane glycoprotein (WFS1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118770.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGAAGGCAG ATGTGGAGGT GCCCTTTGGA GACGTGCTGG AGAGGGCCAA GGCAGGGGAC 
    GCCAAGGCGC AGACGGAGGC GGGGAAGCAC TACCTGCTCC TGGCGGGCAC CGGGGACGAG
    GAGCTGAACA ACTGCACGGC TGTCGACTGG CTCATCCTCG CTGCCAAGCA GGGCCGGCGC
    GAGGCTGTCA AGCTCCTGCG CAGGTGCCTG GCCGACAGGA AAGGCATCAC TTTTGAGAAT
    GAGCAGGAGG TGAAGCAGCT CTCCTCTGAG ACCGACCTGG GAGCGGGGCG GTGCGCAAGG
    CGGGCCCTGG TCATGTACTG GGAAGCTCAA CCCCCAAGAA GAAAGAAGCA GGTGGCCGTG
    TCCGAGCTGC TGGAGAACGT GGGTCAGGTC AACGAGCAGG AGGGAGCGGC ACAGCCGGGC
    CCCATCCCCA AGTCCCTCCA GAAGCAGAGG CGGGTGCTGG AGCGCTTGGT CAGCAGCGAA
    TCCAAGAGCC ACATAGCGCC GGACGACTTC GTGGAGATCA CCAAGAAGTA CGCCAAGGGC
    ATCATCCCCG CCAACCTGTT CCTGCAGGAC GACGACGACG ACGAGCTGGC CGGCAAGGGC
    CCCGAGGACC TGCCCCTGCG TCTGAAGATG GTCAAGTACC CGTTGCACGC TGTCATGGAG
    ATCAAGGAGT ACCTGATCGA TGTGGCCTCG CGGGCGGGCA TGCACTGGCT GTCCAGCATC
    GTGCCCACCC AGCACATCAA CGCCCTCATC TTCTTCTTCA TCATCAGCAA CCTGACCATC
    GACTTCTTCG CCTTCTTCAT CCCGCTGGTC GTCTTCTACC TGTCGTTCGT CTCCATGGTG
    ATCTGCACCC TCAAGGTGTT CCAGGACAGC AGGGCGTGGG AGAGCTTCCG CACCCTCACG
    GGCCTGCTCC TGCGCTTCGA GCCCAACCTG GACGTGGAGC AGGCCGAGGT CAACTTCGGC
    TGGAACCACC TGGAGCCCTA CGTGCACTTC CTGCTGTCTG TCTTCTTCGT CATCTTCTCC
    TTCCCCATCG CCAGCAAGGA CTGCATCCCC TGCTCTGAGC TGGCCGTCGT GTCCATCTTC
    TTCACCGCCA CCAGCTACGT GAGCCTGAGC TCGTCTGCCG AGCCCTACAC CCGGAGGGCC
    CTGGTGACCG AGGTGGCCGC CGGCCTACTG TCCCTCCTGC CCACCCTGCC CTGCGGCTGG
    CAGCACCTGA AGCTCCTGGG CCAGACCTTC GTCACCGTGC CCCTGGGCCA CTTGGTCGTC
    CTGAACGTCA GCGTCCCCTG CCTGCTGTAC GTGTACCTCC TGTACCTCTT CTTCCGCATG
    GCGCAGCTGC GGGGCTTCAA GGGCACCTAC TGCTACCTGG TGCCCTACCT GGTCTGCTTT
    ATGTGGTGTG AGCTGTCCGT GGTCATCCTG CTCGAGTCCA CCGGCCTGGG GCTTGCCCGA
    GCCACCGTGG GCTACTTCCT CTTCCTGTTC GCGCTCCCCG TCCTGGTGGC CGGCCTGGCG
    CTCATGGGCG TGCTGCAGCT TGCCCGCTGG TTCCTGTCGC TGGAGCTCAC CAAGGTGGCC
    GTCACTGCGC TGCTGTGCAG CGTCCCCCTG CTGTTCCGCT GGTGGAGCAA GGCCAGCTTC
    TCGCCGGTGG CCATGGTCAA GTCCCTGACG CGGAGCTCCG TCGTCAAGCT CATCCTGGTG
    TGGATCACGG CCATCGTGCT CTTCTGCTGG CTGTATGTGT ACCGCTCGGA GGGCATGAAG
    GTCTACAACT CCACGCTCAC CTGGCAGCAG TACGGCTTCC TGTGCGGGCC CCGGGCCTGG
    AAGGAGACCA ACATGGCCCG CACCCAGATC CTGTGCAGCC ACCTGGAAGG CCACAGGGTC
    ACATGGACGG GCCGCTTCAA GTACGTGCGG GTGACGGAGA TCGACAACAG CGCCGAGGCG
    GCCATCAACA TGCTCCCGCT CTTCGTGGGC GACTGGATGC GCTGCCTGTA CGGCGAGGCC
    TACCCGTCCT GCAGCCCCGG CAATGCCTCC ACGGCCGCGG AGGAGCTATG CCGCCTGAAG
    CTCCTGGCCA AGCACCCCTG CCACATCCGC AAGTTCGACC GCTACAAGTT CGAGATCACG
    GTGGGCATGC CCTACAGTGG CGCCAACGGC TCCCGCGGCC CAGAGGAGGA CGACGTCACC
    AAGGACATCG TGCTGCGGGC CAGCGGCGAG TTCAAGGACG TGCTTCTCGG CCTGCGCCAC
    GGCAGCCTCA TCGAGTTCAG CACCGTCCTC GAGGGCCGCC TGGGCAGCAA GTGGCCCGTC
    TTCGAGCTCA AGGCCATCCG CTGCCTCAAC TGCATGGCAC AGCTGTCCCC CGCCCGGCGC
    CACGTGAAGA TAGAGCACGA CTGGCGCAGC ACCGTGCACG GCGCCGTCAA GTTCGCCTTT
    GACTTCTTCT TCTTCCCGTT CCTGTCGGCC GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39784
    Clone ID Related Accession (Same CDS sequence) XM_014118771.1 , XM_014118772.2 , XM_014118772.1 , XM_014118770.2 , XM_014118771.2 , XM_014118770.1 , XM_014118773.1
    Accession Version XM_014118771.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2436bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product wolframin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014118771.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGAAGGCAG ATGTGGAGGT GCCCTTTGGA GACGTGCTGG AGAGGGCCAA GGCAGGGGAC 
    GCCAAGGCGC AGACGGAGGC GGGGAAGCAC TACCTGCTCC TGGCGGGCAC CGGGGACGAG
    GAGCTGAACA ACTGCACGGC TGTCGACTGG CTCATCCTCG CTGCCAAGCA GGGCCGGCGC
    GAGGCTGTCA AGCTCCTGCG CAGGTGCCTG GCCGACAGGA AAGGCATCAC TTTTGAGAAT
    GAGCAGGAGG TGAAGCAGCT CTCCTCTGAG ACCGACCTGG GAGCGGGGCG GTGCGCAAGG
    CGGGCCCTGG TCATGTACTG GGAAGCTCAA CCCCCAAGAA GAAAGAAGCA GGTGGCCGTG
    TCCGAGCTGC TGGAGAACGT GGGTCAGGTC AACGAGCAGG AGGGAGCGGC ACAGCCGGGC
    CCCATCCCCA AGTCCCTCCA GAAGCAGAGG CGGGTGCTGG AGCGCTTGGT CAGCAGCGAA
    TCCAAGAGCC ACATAGCGCC GGACGACTTC GTGGAGATCA CCAAGAAGTA CGCCAAGGGC
    ATCATCCCCG CCAACCTGTT CCTGCAGGAC GACGACGACG ACGAGCTGGC CGGCAAGGGC
    CCCGAGGACC TGCCCCTGCG TCTGAAGATG GTCAAGTACC CGTTGCACGC TGTCATGGAG
    ATCAAGGAGT ACCTGATCGA TGTGGCCTCG CGGGCGGGCA TGCACTGGCT GTCCAGCATC
    GTGCCCACCC AGCACATCAA CGCCCTCATC TTCTTCTTCA TCATCAGCAA CCTGACCATC
    GACTTCTTCG CCTTCTTCAT CCCGCTGGTC GTCTTCTACC TGTCGTTCGT CTCCATGGTG
    ATCTGCACCC TCAAGGTGTT CCAGGACAGC AGGGCGTGGG AGAGCTTCCG CACCCTCACG
    GGCCTGCTCC TGCGCTTCGA GCCCAACCTG GACGTGGAGC AGGCCGAGGT CAACTTCGGC
    TGGAACCACC TGGAGCCCTA CGTGCACTTC CTGCTGTCTG TCTTCTTCGT CATCTTCTCC
    TTCCCCATCG CCAGCAAGGA CTGCATCCCC TGCTCTGAGC TGGCCGTCGT GTCCATCTTC
    TTCACCGCCA CCAGCTACGT GAGCCTGAGC TCGTCTGCCG AGCCCTACAC CCGGAGGGCC
    CTGGTGACCG AGGTGGCCGC CGGCCTACTG TCCCTCCTGC CCACCCTGCC CTGCGGCTGG
    CAGCACCTGA AGCTCCTGGG CCAGACCTTC GTCACCGTGC CCCTGGGCCA CTTGGTCGTC
    CTGAACGTCA GCGTCCCCTG CCTGCTGTAC GTGTACCTCC TGTACCTCTT CTTCCGCATG
    GCGCAGCTGC GGGGCTTCAA GGGCACCTAC TGCTACCTGG TGCCCTACCT GGTCTGCTTT
    ATGTGGTGTG AGCTGTCCGT GGTCATCCTG CTCGAGTCCA CCGGCCTGGG GCTTGCCCGA
    GCCACCGTGG GCTACTTCCT CTTCCTGTTC GCGCTCCCCG TCCTGGTGGC CGGCCTGGCG
    CTCATGGGCG TGCTGCAGCT TGCCCGCTGG TTCCTGTCGC TGGAGCTCAC CAAGGTGGCC
    GTCACTGCGC TGCTGTGCAG CGTCCCCCTG CTGTTCCGCT GGTGGAGCAA GGCCAGCTTC
    TCGCCGGTGG CCATGGTCAA GTCCCTGACG CGGAGCTCCG TCGTCAAGCT CATCCTGGTG
    TGGATCACGG CCATCGTGCT CTTCTGCTGG CTGTATGTGT ACCGCTCGGA GGGCATGAAG
    GTCTACAACT CCACGCTCAC CTGGCAGCAG TACGGCTTCC TGTGCGGGCC CCGGGCCTGG
    AAGGAGACCA ACATGGCCCG CACCCAGATC CTGTGCAGCC ACCTGGAAGG CCACAGGGTC
    ACATGGACGG GCCGCTTCAA GTACGTGCGG GTGACGGAGA TCGACAACAG CGCCGAGGCG
    GCCATCAACA TGCTCCCGCT CTTCGTGGGC GACTGGATGC GCTGCCTGTA CGGCGAGGCC
    TACCCGTCCT GCAGCCCCGG CAATGCCTCC ACGGCCGCGG AGGAGCTATG CCGCCTGAAG
    CTCCTGGCCA AGCACCCCTG CCACATCCGC AAGTTCGACC GCTACAAGTT CGAGATCACG
    GTGGGCATGC CCTACAGTGG CGCCAACGGC TCCCGCGGCC CAGAGGAGGA CGACGTCACC
    AAGGACATCG TGCTGCGGGC CAGCGGCGAG TTCAAGGACG TGCTTCTCGG CCTGCGCCAC
    GGCAGCCTCA TCGAGTTCAG CACCGTCCTC GAGGGCCGCC TGGGCAGCAA GTGGCCCGTC
    TTCGAGCTCA AGGCCATCCG CTGCCTCAAC TGCATGGCAC AGCTGTCCCC CGCCCGGCGC
    CACGTGAAGA TAGAGCACGA CTGGCGCAGC ACCGTGCACG GCGCCGTCAA GTTCGCCTTT
    GACTTCTTCT TCTTCCCGTT CCTGTCGGCC GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974246.1
    CDS1771..4206
    Translation

    Target ORF information:

    RefSeq Version XM_014118771.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris wolframin ER transmembrane glycoprotein (WFS1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118771.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGAAGGCAG ATGTGGAGGT GCCCTTTGGA GACGTGCTGG AGAGGGCCAA GGCAGGGGAC 
    GCCAAGGCGC AGACGGAGGC GGGGAAGCAC TACCTGCTCC TGGCGGGCAC CGGGGACGAG
    GAGCTGAACA ACTGCACGGC TGTCGACTGG CTCATCCTCG CTGCCAAGCA GGGCCGGCGC
    GAGGCTGTCA AGCTCCTGCG CAGGTGCCTG GCCGACAGGA AAGGCATCAC TTTTGAGAAT
    GAGCAGGAGG TGAAGCAGCT CTCCTCTGAG ACCGACCTGG GAGCGGGGCG GTGCGCAAGG
    CGGGCCCTGG TCATGTACTG GGAAGCTCAA CCCCCAAGAA GAAAGAAGCA GGTGGCCGTG
    TCCGAGCTGC TGGAGAACGT GGGTCAGGTC AACGAGCAGG AGGGAGCGGC ACAGCCGGGC
    CCCATCCCCA AGTCCCTCCA GAAGCAGAGG CGGGTGCTGG AGCGCTTGGT CAGCAGCGAA
    TCCAAGAGCC ACATAGCGCC GGACGACTTC GTGGAGATCA CCAAGAAGTA CGCCAAGGGC
    ATCATCCCCG CCAACCTGTT CCTGCAGGAC GACGACGACG ACGAGCTGGC CGGCAAGGGC
    CCCGAGGACC TGCCCCTGCG TCTGAAGATG GTCAAGTACC CGTTGCACGC TGTCATGGAG
    ATCAAGGAGT ACCTGATCGA TGTGGCCTCG CGGGCGGGCA TGCACTGGCT GTCCAGCATC
    GTGCCCACCC AGCACATCAA CGCCCTCATC TTCTTCTTCA TCATCAGCAA CCTGACCATC
    GACTTCTTCG CCTTCTTCAT CCCGCTGGTC GTCTTCTACC TGTCGTTCGT CTCCATGGTG
    ATCTGCACCC TCAAGGTGTT CCAGGACAGC AGGGCGTGGG AGAGCTTCCG CACCCTCACG
    GGCCTGCTCC TGCGCTTCGA GCCCAACCTG GACGTGGAGC AGGCCGAGGT CAACTTCGGC
    TGGAACCACC TGGAGCCCTA CGTGCACTTC CTGCTGTCTG TCTTCTTCGT CATCTTCTCC
    TTCCCCATCG CCAGCAAGGA CTGCATCCCC TGCTCTGAGC TGGCCGTCGT GTCCATCTTC
    TTCACCGCCA CCAGCTACGT GAGCCTGAGC TCGTCTGCCG AGCCCTACAC CCGGAGGGCC
    CTGGTGACCG AGGTGGCCGC CGGCCTACTG TCCCTCCTGC CCACCCTGCC CTGCGGCTGG
    CAGCACCTGA AGCTCCTGGG CCAGACCTTC GTCACCGTGC CCCTGGGCCA CTTGGTCGTC
    CTGAACGTCA GCGTCCCCTG CCTGCTGTAC GTGTACCTCC TGTACCTCTT CTTCCGCATG
    GCGCAGCTGC GGGGCTTCAA GGGCACCTAC TGCTACCTGG TGCCCTACCT GGTCTGCTTT
    ATGTGGTGTG AGCTGTCCGT GGTCATCCTG CTCGAGTCCA CCGGCCTGGG GCTTGCCCGA
    GCCACCGTGG GCTACTTCCT CTTCCTGTTC GCGCTCCCCG TCCTGGTGGC CGGCCTGGCG
    CTCATGGGCG TGCTGCAGCT TGCCCGCTGG TTCCTGTCGC TGGAGCTCAC CAAGGTGGCC
    GTCACTGCGC TGCTGTGCAG CGTCCCCCTG CTGTTCCGCT GGTGGAGCAA GGCCAGCTTC
    TCGCCGGTGG CCATGGTCAA GTCCCTGACG CGGAGCTCCG TCGTCAAGCT CATCCTGGTG
    TGGATCACGG CCATCGTGCT CTTCTGCTGG CTGTATGTGT ACCGCTCGGA GGGCATGAAG
    GTCTACAACT CCACGCTCAC CTGGCAGCAG TACGGCTTCC TGTGCGGGCC CCGGGCCTGG
    AAGGAGACCA ACATGGCCCG CACCCAGATC CTGTGCAGCC ACCTGGAAGG CCACAGGGTC
    ACATGGACGG GCCGCTTCAA GTACGTGCGG GTGACGGAGA TCGACAACAG CGCCGAGGCG
    GCCATCAACA TGCTCCCGCT CTTCGTGGGC GACTGGATGC GCTGCCTGTA CGGCGAGGCC
    TACCCGTCCT GCAGCCCCGG CAATGCCTCC ACGGCCGCGG AGGAGCTATG CCGCCTGAAG
    CTCCTGGCCA AGCACCCCTG CCACATCCGC AAGTTCGACC GCTACAAGTT CGAGATCACG
    GTGGGCATGC CCTACAGTGG CGCCAACGGC TCCCGCGGCC CAGAGGAGGA CGACGTCACC
    AAGGACATCG TGCTGCGGGC CAGCGGCGAG TTCAAGGACG TGCTTCTCGG CCTGCGCCAC
    GGCAGCCTCA TCGAGTTCAG CACCGTCCTC GAGGGCCGCC TGGGCAGCAA GTGGCCCGTC
    TTCGAGCTCA AGGCCATCCG CTGCCTCAAC TGCATGGCAC AGCTGTCCCC CGCCCGGCGC
    CACGTGAAGA TAGAGCACGA CTGGCGCAGC ACCGTGCACG GCGCCGTCAA GTTCGCCTTT
    GACTTCTTCT TCTTCCCGTT CCTGTCGGCC GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39784
    Clone ID Related Accession (Same CDS sequence) XM_014118771.1 , XM_014118772.2 , XM_014118772.1 , XM_014118770.2 , XM_014118771.2 , XM_014118770.1 , XM_014118773.1
    Accession Version XM_014118770.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2436bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product wolframin isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726083.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGAAGGCAG ATGTGGAGGT GCCCTTTGGA GACGTGCTGG AGAGGGCCAA GGCAGGGGAC 
    GCCAAGGCGC AGACGGAGGC GGGGAAGCAC TACCTGCTCC TGGCGGGCAC CGGGGACGAG
    GAGCTGAACA ACTGCACGGC TGTCGACTGG CTCATCCTCG CTGCCAAGCA GGGCCGGCGC
    GAGGCTGTCA AGCTCCTGCG CAGGTGCCTG GCCGACAGGA AAGGCATCAC TTTTGAGAAT
    GAGCAGGAGG TGAAGCAGCT CTCCTCTGAG ACCGACCTGG GAGCGGGGCG GTGCGCAAGG
    CGGGCCCTGG TCATGTACTG GGAAGCTCAA CCCCCAAGAA GAAAGAAGCA GGTGGCCGTG
    TCCGAGCTGC TGGAGAACGT GGGTCAGGTC AACGAGCAGG AGGGAGCGGC ACAGCCGGGC
    CCCATCCCCA AGTCCCTCCA GAAGCAGAGG CGGGTGCTGG AGCGCTTGGT CAGCAGCGAA
    TCCAAGAGCC ACATAGCGCC GGACGACTTC GTGGAGATCA CCAAGAAGTA CGCCAAGGGC
    ATCATCCCCG CCAACCTGTT CCTGCAGGAC GACGACGACG ACGAGCTGGC CGGCAAGGGC
    CCCGAGGACC TGCCCCTGCG TCTGAAGATG GTCAAGTACC CGTTGCACGC TGTCATGGAG
    ATCAAGGAGT ACCTGATCGA TGTGGCCTCG CGGGCGGGCA TGCACTGGCT GTCCAGCATC
    GTGCCCACCC AGCACATCAA CGCCCTCATC TTCTTCTTCA TCATCAGCAA CCTGACCATC
    GACTTCTTCG CCTTCTTCAT CCCGCTGGTC GTCTTCTACC TGTCGTTCGT CTCCATGGTG
    ATCTGCACCC TCAAGGTGTT CCAGGACAGC AGGGCGTGGG AGAGCTTCCG CACCCTCACG
    GGCCTGCTCC TGCGCTTCGA GCCCAACCTG GACGTGGAGC AGGCCGAGGT CAACTTCGGC
    TGGAACCACC TGGAGCCCTA CGTGCACTTC CTGCTGTCTG TCTTCTTCGT CATCTTCTCC
    TTCCCCATCG CCAGCAAGGA CTGCATCCCC TGCTCTGAGC TGGCCGTCGT GTCCATCTTC
    TTCACCGCCA CCAGCTACGT GAGCCTGAGC TCGTCTGCCG AGCCCTACAC CCGGAGGGCC
    CTGGTGACCG AGGTGGCCGC CGGCCTACTG TCCCTCCTGC CCACCCTGCC CTGCGGCTGG
    CAGCACCTGA AGCTCCTGGG CCAGACCTTC GTCACCGTGC CCCTGGGCCA CTTGGTCGTC
    CTGAACGTCA GCGTCCCCTG CCTGCTGTAC GTGTACCTCC TGTACCTCTT CTTCCGCATG
    GCGCAGCTGC GGGGCTTCAA GGGCACCTAC TGCTACCTGG TGCCCTACCT GGTCTGCTTT
    ATGTGGTGTG AGCTGTCCGT GGTCATCCTG CTCGAGTCCA CCGGCCTGGG GCTTGCCCGA
    GCCACCGTGG GCTACTTCCT CTTCCTGTTC GCGCTCCCCG TCCTGGTGGC CGGCCTGGCG
    CTCATGGGCG TGCTGCAGCT TGCCCGCTGG TTCCTGTCGC TGGAGCTCAC CAAGGTGGCC
    GTCACTGCGC TGCTGTGCAG CGTCCCCCTG CTGTTCCGCT GGTGGAGCAA GGCCAGCTTC
    TCGCCGGTGG CCATGGTCAA GTCCCTGACG CGGAGCTCCG TCGTCAAGCT CATCCTGGTG
    TGGATCACGG CCATCGTGCT CTTCTGCTGG CTGTATGTGT ACCGCTCGGA GGGCATGAAG
    GTCTACAACT CCACGCTCAC CTGGCAGCAG TACGGCTTCC TGTGCGGGCC CCGGGCCTGG
    AAGGAGACCA ACATGGCCCG CACCCAGATC CTGTGCAGCC ACCTGGAAGG CCACAGGGTC
    ACATGGACGG GCCGCTTCAA GTACGTGCGG GTGACGGAGA TCGACAACAG CGCCGAGGCG
    GCCATCAACA TGCTCCCGCT CTTCGTGGGC GACTGGATGC GCTGCCTGTA CGGCGAGGCC
    TACCCGTCCT GCAGCCCCGG CAATGCCTCC ACGGCCGCGG AGGAGCTATG CCGCCTGAAG
    CTCCTGGCCA AGCACCCCTG CCACATCCGC AAGTTCGACC GCTACAAGTT CGAGATCACG
    GTGGGCATGC CCTACAGTGG CGCCAACGGC TCCCGCGGCC CAGAGGAGGA CGACGTCACC
    AAGGACATCG TGCTGCGGGC CAGCGGCGAG TTCAAGGACG TGCTTCTCGG CCTGCGCCAC
    GGCAGCCTCA TCGAGTTCAG CACCGTCCTC GAGGGCCGCC TGGGCAGCAA GTGGCCCGTC
    TTCGAGCTCA AGGCCATCCG CTGCCTCAAC TGCATGGCAC AGCTGTCCCC CGCCCGGCGC
    CACGTGAAGA TAGAGCACGA CTGGCGCAGC ACCGTGCACG GCGCCGTCAA GTTCGCCTTT
    GACTTCTTCT TCTTCCCGTT CCTGTCGGCC GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974245.1
    CDS1840..4275
    Translation

    Target ORF information:

    RefSeq Version XM_014118770.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Wolfram syndrome 1 (wolframin) (WFS1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118770.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGAAGGCAG ATGTGGAGGT GCCCTTTGGA GACGTGCTGG AGAGGGCCAA GGCAGGGGAC 
    GCCAAGGCGC AGACGGAGGC GGGGAAGCAC TACCTGCTCC TGGCGGGCAC CGGGGACGAG
    GAGCTGAACA ACTGCACGGC TGTCGACTGG CTCATCCTCG CTGCCAAGCA GGGCCGGCGC
    GAGGCTGTCA AGCTCCTGCG CAGGTGCCTG GCCGACAGGA AAGGCATCAC TTTTGAGAAT
    GAGCAGGAGG TGAAGCAGCT CTCCTCTGAG ACCGACCTGG GAGCGGGGCG GTGCGCAAGG
    CGGGCCCTGG TCATGTACTG GGAAGCTCAA CCCCCAAGAA GAAAGAAGCA GGTGGCCGTG
    TCCGAGCTGC TGGAGAACGT GGGTCAGGTC AACGAGCAGG AGGGAGCGGC ACAGCCGGGC
    CCCATCCCCA AGTCCCTCCA GAAGCAGAGG CGGGTGCTGG AGCGCTTGGT CAGCAGCGAA
    TCCAAGAGCC ACATAGCGCC GGACGACTTC GTGGAGATCA CCAAGAAGTA CGCCAAGGGC
    ATCATCCCCG CCAACCTGTT CCTGCAGGAC GACGACGACG ACGAGCTGGC CGGCAAGGGC
    CCCGAGGACC TGCCCCTGCG TCTGAAGATG GTCAAGTACC CGTTGCACGC TGTCATGGAG
    ATCAAGGAGT ACCTGATCGA TGTGGCCTCG CGGGCGGGCA TGCACTGGCT GTCCAGCATC
    GTGCCCACCC AGCACATCAA CGCCCTCATC TTCTTCTTCA TCATCAGCAA CCTGACCATC
    GACTTCTTCG CCTTCTTCAT CCCGCTGGTC GTCTTCTACC TGTCGTTCGT CTCCATGGTG
    ATCTGCACCC TCAAGGTGTT CCAGGACAGC AGGGCGTGGG AGAGCTTCCG CACCCTCACG
    GGCCTGCTCC TGCGCTTCGA GCCCAACCTG GACGTGGAGC AGGCCGAGGT CAACTTCGGC
    TGGAACCACC TGGAGCCCTA CGTGCACTTC CTGCTGTCTG TCTTCTTCGT CATCTTCTCC
    TTCCCCATCG CCAGCAAGGA CTGCATCCCC TGCTCTGAGC TGGCCGTCGT GTCCATCTTC
    TTCACCGCCA CCAGCTACGT GAGCCTGAGC TCGTCTGCCG AGCCCTACAC CCGGAGGGCC
    CTGGTGACCG AGGTGGCCGC CGGCCTACTG TCCCTCCTGC CCACCCTGCC CTGCGGCTGG
    CAGCACCTGA AGCTCCTGGG CCAGACCTTC GTCACCGTGC CCCTGGGCCA CTTGGTCGTC
    CTGAACGTCA GCGTCCCCTG CCTGCTGTAC GTGTACCTCC TGTACCTCTT CTTCCGCATG
    GCGCAGCTGC GGGGCTTCAA GGGCACCTAC TGCTACCTGG TGCCCTACCT GGTCTGCTTT
    ATGTGGTGTG AGCTGTCCGT GGTCATCCTG CTCGAGTCCA CCGGCCTGGG GCTTGCCCGA
    GCCACCGTGG GCTACTTCCT CTTCCTGTTC GCGCTCCCCG TCCTGGTGGC CGGCCTGGCG
    CTCATGGGCG TGCTGCAGCT TGCCCGCTGG TTCCTGTCGC TGGAGCTCAC CAAGGTGGCC
    GTCACTGCGC TGCTGTGCAG CGTCCCCCTG CTGTTCCGCT GGTGGAGCAA GGCCAGCTTC
    TCGCCGGTGG CCATGGTCAA GTCCCTGACG CGGAGCTCCG TCGTCAAGCT CATCCTGGTG
    TGGATCACGG CCATCGTGCT CTTCTGCTGG CTGTATGTGT ACCGCTCGGA GGGCATGAAG
    GTCTACAACT CCACGCTCAC CTGGCAGCAG TACGGCTTCC TGTGCGGGCC CCGGGCCTGG
    AAGGAGACCA ACATGGCCCG CACCCAGATC CTGTGCAGCC ACCTGGAAGG CCACAGGGTC
    ACATGGACGG GCCGCTTCAA GTACGTGCGG GTGACGGAGA TCGACAACAG CGCCGAGGCG
    GCCATCAACA TGCTCCCGCT CTTCGTGGGC GACTGGATGC GCTGCCTGTA CGGCGAGGCC
    TACCCGTCCT GCAGCCCCGG CAATGCCTCC ACGGCCGCGG AGGAGCTATG CCGCCTGAAG
    CTCCTGGCCA AGCACCCCTG CCACATCCGC AAGTTCGACC GCTACAAGTT CGAGATCACG
    GTGGGCATGC CCTACAGTGG CGCCAACGGC TCCCGCGGCC CAGAGGAGGA CGACGTCACC
    AAGGACATCG TGCTGCGGGC CAGCGGCGAG TTCAAGGACG TGCTTCTCGG CCTGCGCCAC
    GGCAGCCTCA TCGAGTTCAG CACCGTCCTC GAGGGCCGCC TGGGCAGCAA GTGGCCCGTC
    TTCGAGCTCA AGGCCATCCG CTGCCTCAAC TGCATGGCAC AGCTGTCCCC CGCCCGGCGC
    CACGTGAAGA TAGAGCACGA CTGGCGCAGC ACCGTGCACG GCGCCGTCAA GTTCGCCTTT
    GACTTCTTCT TCTTCCCGTT CCTGTCGGCC GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39784
    Clone ID Related Accession (Same CDS sequence) XM_014118771.1 , XM_014118772.2 , XM_014118772.1 , XM_014118770.2 , XM_014118771.2 , XM_014118770.1 , XM_014118773.1
    Accession Version XM_014118773.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2436bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product wolframin isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726083.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGAAGGCAG ATGTGGAGGT GCCCTTTGGA GACGTGCTGG AGAGGGCCAA GGCAGGGGAC 
    GCCAAGGCGC AGACGGAGGC GGGGAAGCAC TACCTGCTCC TGGCGGGCAC CGGGGACGAG
    GAGCTGAACA ACTGCACGGC TGTCGACTGG CTCATCCTCG CTGCCAAGCA GGGCCGGCGC
    GAGGCTGTCA AGCTCCTGCG CAGGTGCCTG GCCGACAGGA AAGGCATCAC TTTTGAGAAT
    GAGCAGGAGG TGAAGCAGCT CTCCTCTGAG ACCGACCTGG GAGCGGGGCG GTGCGCAAGG
    CGGGCCCTGG TCATGTACTG GGAAGCTCAA CCCCCAAGAA GAAAGAAGCA GGTGGCCGTG
    TCCGAGCTGC TGGAGAACGT GGGTCAGGTC AACGAGCAGG AGGGAGCGGC ACAGCCGGGC
    CCCATCCCCA AGTCCCTCCA GAAGCAGAGG CGGGTGCTGG AGCGCTTGGT CAGCAGCGAA
    TCCAAGAGCC ACATAGCGCC GGACGACTTC GTGGAGATCA CCAAGAAGTA CGCCAAGGGC
    ATCATCCCCG CCAACCTGTT CCTGCAGGAC GACGACGACG ACGAGCTGGC CGGCAAGGGC
    CCCGAGGACC TGCCCCTGCG TCTGAAGATG GTCAAGTACC CGTTGCACGC TGTCATGGAG
    ATCAAGGAGT ACCTGATCGA TGTGGCCTCG CGGGCGGGCA TGCACTGGCT GTCCAGCATC
    GTGCCCACCC AGCACATCAA CGCCCTCATC TTCTTCTTCA TCATCAGCAA CCTGACCATC
    GACTTCTTCG CCTTCTTCAT CCCGCTGGTC GTCTTCTACC TGTCGTTCGT CTCCATGGTG
    ATCTGCACCC TCAAGGTGTT CCAGGACAGC AGGGCGTGGG AGAGCTTCCG CACCCTCACG
    GGCCTGCTCC TGCGCTTCGA GCCCAACCTG GACGTGGAGC AGGCCGAGGT CAACTTCGGC
    TGGAACCACC TGGAGCCCTA CGTGCACTTC CTGCTGTCTG TCTTCTTCGT CATCTTCTCC
    TTCCCCATCG CCAGCAAGGA CTGCATCCCC TGCTCTGAGC TGGCCGTCGT GTCCATCTTC
    TTCACCGCCA CCAGCTACGT GAGCCTGAGC TCGTCTGCCG AGCCCTACAC CCGGAGGGCC
    CTGGTGACCG AGGTGGCCGC CGGCCTACTG TCCCTCCTGC CCACCCTGCC CTGCGGCTGG
    CAGCACCTGA AGCTCCTGGG CCAGACCTTC GTCACCGTGC CCCTGGGCCA CTTGGTCGTC
    CTGAACGTCA GCGTCCCCTG CCTGCTGTAC GTGTACCTCC TGTACCTCTT CTTCCGCATG
    GCGCAGCTGC GGGGCTTCAA GGGCACCTAC TGCTACCTGG TGCCCTACCT GGTCTGCTTT
    ATGTGGTGTG AGCTGTCCGT GGTCATCCTG CTCGAGTCCA CCGGCCTGGG GCTTGCCCGA
    GCCACCGTGG GCTACTTCCT CTTCCTGTTC GCGCTCCCCG TCCTGGTGGC CGGCCTGGCG
    CTCATGGGCG TGCTGCAGCT TGCCCGCTGG TTCCTGTCGC TGGAGCTCAC CAAGGTGGCC
    GTCACTGCGC TGCTGTGCAG CGTCCCCCTG CTGTTCCGCT GGTGGAGCAA GGCCAGCTTC
    TCGCCGGTGG CCATGGTCAA GTCCCTGACG CGGAGCTCCG TCGTCAAGCT CATCCTGGTG
    TGGATCACGG CCATCGTGCT CTTCTGCTGG CTGTATGTGT ACCGCTCGGA GGGCATGAAG
    GTCTACAACT CCACGCTCAC CTGGCAGCAG TACGGCTTCC TGTGCGGGCC CCGGGCCTGG
    AAGGAGACCA ACATGGCCCG CACCCAGATC CTGTGCAGCC ACCTGGAAGG CCACAGGGTC
    ACATGGACGG GCCGCTTCAA GTACGTGCGG GTGACGGAGA TCGACAACAG CGCCGAGGCG
    GCCATCAACA TGCTCCCGCT CTTCGTGGGC GACTGGATGC GCTGCCTGTA CGGCGAGGCC
    TACCCGTCCT GCAGCCCCGG CAATGCCTCC ACGGCCGCGG AGGAGCTATG CCGCCTGAAG
    CTCCTGGCCA AGCACCCCTG CCACATCCGC AAGTTCGACC GCTACAAGTT CGAGATCACG
    GTGGGCATGC CCTACAGTGG CGCCAACGGC TCCCGCGGCC CAGAGGAGGA CGACGTCACC
    AAGGACATCG TGCTGCGGGC CAGCGGCGAG TTCAAGGACG TGCTTCTCGG CCTGCGCCAC
    GGCAGCCTCA TCGAGTTCAG CACCGTCCTC GAGGGCCGCC TGGGCAGCAA GTGGCCCGTC
    TTCGAGCTCA AGGCCATCCG CTGCCTCAAC TGCATGGCAC AGCTGTCCCC CGCCCGGCGC
    CACGTGAAGA TAGAGCACGA CTGGCGCAGC ACCGTGCACG GCGCCGTCAA GTTCGCCTTT
    GACTTCTTCT TCTTCCCGTT CCTGTCGGCC GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974248.1
    CDS1400..3835
    Translation

    Target ORF information:

    RefSeq Version XM_014118773.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Wolfram syndrome 1 (wolframin) (WFS1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118773.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGAAGGCAG ATGTGGAGGT GCCCTTTGGA GACGTGCTGG AGAGGGCCAA GGCAGGGGAC 
    GCCAAGGCGC AGACGGAGGC GGGGAAGCAC TACCTGCTCC TGGCGGGCAC CGGGGACGAG
    GAGCTGAACA ACTGCACGGC TGTCGACTGG CTCATCCTCG CTGCCAAGCA GGGCCGGCGC
    GAGGCTGTCA AGCTCCTGCG CAGGTGCCTG GCCGACAGGA AAGGCATCAC TTTTGAGAAT
    GAGCAGGAGG TGAAGCAGCT CTCCTCTGAG ACCGACCTGG GAGCGGGGCG GTGCGCAAGG
    CGGGCCCTGG TCATGTACTG GGAAGCTCAA CCCCCAAGAA GAAAGAAGCA GGTGGCCGTG
    TCCGAGCTGC TGGAGAACGT GGGTCAGGTC AACGAGCAGG AGGGAGCGGC ACAGCCGGGC
    CCCATCCCCA AGTCCCTCCA GAAGCAGAGG CGGGTGCTGG AGCGCTTGGT CAGCAGCGAA
    TCCAAGAGCC ACATAGCGCC GGACGACTTC GTGGAGATCA CCAAGAAGTA CGCCAAGGGC
    ATCATCCCCG CCAACCTGTT CCTGCAGGAC GACGACGACG ACGAGCTGGC CGGCAAGGGC
    CCCGAGGACC TGCCCCTGCG TCTGAAGATG GTCAAGTACC CGTTGCACGC TGTCATGGAG
    ATCAAGGAGT ACCTGATCGA TGTGGCCTCG CGGGCGGGCA TGCACTGGCT GTCCAGCATC
    GTGCCCACCC AGCACATCAA CGCCCTCATC TTCTTCTTCA TCATCAGCAA CCTGACCATC
    GACTTCTTCG CCTTCTTCAT CCCGCTGGTC GTCTTCTACC TGTCGTTCGT CTCCATGGTG
    ATCTGCACCC TCAAGGTGTT CCAGGACAGC AGGGCGTGGG AGAGCTTCCG CACCCTCACG
    GGCCTGCTCC TGCGCTTCGA GCCCAACCTG GACGTGGAGC AGGCCGAGGT CAACTTCGGC
    TGGAACCACC TGGAGCCCTA CGTGCACTTC CTGCTGTCTG TCTTCTTCGT CATCTTCTCC
    TTCCCCATCG CCAGCAAGGA CTGCATCCCC TGCTCTGAGC TGGCCGTCGT GTCCATCTTC
    TTCACCGCCA CCAGCTACGT GAGCCTGAGC TCGTCTGCCG AGCCCTACAC CCGGAGGGCC
    CTGGTGACCG AGGTGGCCGC CGGCCTACTG TCCCTCCTGC CCACCCTGCC CTGCGGCTGG
    CAGCACCTGA AGCTCCTGGG CCAGACCTTC GTCACCGTGC CCCTGGGCCA CTTGGTCGTC
    CTGAACGTCA GCGTCCCCTG CCTGCTGTAC GTGTACCTCC TGTACCTCTT CTTCCGCATG
    GCGCAGCTGC GGGGCTTCAA GGGCACCTAC TGCTACCTGG TGCCCTACCT GGTCTGCTTT
    ATGTGGTGTG AGCTGTCCGT GGTCATCCTG CTCGAGTCCA CCGGCCTGGG GCTTGCCCGA
    GCCACCGTGG GCTACTTCCT CTTCCTGTTC GCGCTCCCCG TCCTGGTGGC CGGCCTGGCG
    CTCATGGGCG TGCTGCAGCT TGCCCGCTGG TTCCTGTCGC TGGAGCTCAC CAAGGTGGCC
    GTCACTGCGC TGCTGTGCAG CGTCCCCCTG CTGTTCCGCT GGTGGAGCAA GGCCAGCTTC
    TCGCCGGTGG CCATGGTCAA GTCCCTGACG CGGAGCTCCG TCGTCAAGCT CATCCTGGTG
    TGGATCACGG CCATCGTGCT CTTCTGCTGG CTGTATGTGT ACCGCTCGGA GGGCATGAAG
    GTCTACAACT CCACGCTCAC CTGGCAGCAG TACGGCTTCC TGTGCGGGCC CCGGGCCTGG
    AAGGAGACCA ACATGGCCCG CACCCAGATC CTGTGCAGCC ACCTGGAAGG CCACAGGGTC
    ACATGGACGG GCCGCTTCAA GTACGTGCGG GTGACGGAGA TCGACAACAG CGCCGAGGCG
    GCCATCAACA TGCTCCCGCT CTTCGTGGGC GACTGGATGC GCTGCCTGTA CGGCGAGGCC
    TACCCGTCCT GCAGCCCCGG CAATGCCTCC ACGGCCGCGG AGGAGCTATG CCGCCTGAAG
    CTCCTGGCCA AGCACCCCTG CCACATCCGC AAGTTCGACC GCTACAAGTT CGAGATCACG
    GTGGGCATGC CCTACAGTGG CGCCAACGGC TCCCGCGGCC CAGAGGAGGA CGACGTCACC
    AAGGACATCG TGCTGCGGGC CAGCGGCGAG TTCAAGGACG TGCTTCTCGG CCTGCGCCAC
    GGCAGCCTCA TCGAGTTCAG CACCGTCCTC GAGGGCCGCC TGGGCAGCAA GTGGCCCGTC
    TTCGAGCTCA AGGCCATCCG CTGCCTCAAC TGCATGGCAC AGCTGTCCCC CGCCCGGCGC
    CACGTGAAGA TAGAGCACGA CTGGCGCAGC ACCGTGCACG GCGCCGTCAA GTTCGCCTTT
    GACTTCTTCT TCTTCCCGTT CCTGTCGGCC GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.