MED1 cDNA ORF clone, Canis lupus familiaris(Dog)

The following MED1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe38367 XM_022423023.1
Latest version!
Canis lupus familiaris mediator complex subunit 1 (MED1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe38367 XM_014116603.2
Latest version!
Canis lupus familiaris mediator complex subunit 1 (MED1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe11780 XM_858916.5
Latest version!
Canis lupus familiaris mediator complex subunit 1 (MED1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
View Old Accession Versions >>
OCe11780 XM_858916.4 Canis lupus familiaris mediator complex subunit 1 (MED1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe38367 XM_014116603.1 Canis lupus familiaris mediator complex subunit 1 (MED1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe38367
    Clone ID Related Accession (Same CDS sequence) XM_014116603.2 , XM_022423023.1 , XM_014116603.1
    Accession Version XM_014116603.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4236bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product mediator of RNA polymerase II transcription subunit 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014116603.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTACTTGG CTCTCCAATC CTTAGAACAG GATCTATCTA AAATGGCAGT TATGTATTGG 
    AAAGCAACCA ATGCTGGTCC CTTGGATAAG ATTCTTCATG GAAGTGTTGG CTATCTTACT
    CCAAGAAGTG GGGGTCATTT AATGAACTTG AAGTACTATG CCTCTCCTTC TGACCTGCTG
    GATGATAAGA CTACATCTCC CATCATTTTG CATGAGAATA ATGTTCCTCG ATCATTGGGC
    ATGAACGCAT CAGTGACAAT TGAAGGAACA TCTGCTATGT ATAAACTCCC AATTGCACCA
    TTAATTATGG GGTCACATCC AGTTGACAAC AAATGGACCC CCTCCTTCTC CTCAATCACC
    AGTGCCAACA GTGTTGATCT TCCTGCCTGT TTCTTCTTGA AATTTCCCCA ACCAATCCCA
    GTATCTAGAG CATTTGTTCA GAAACTTCAG AACTGCACAG GAATTCCATT GTTTGAAACT
    CAACCAACTT ATGTACCCCT CTATGAACTG ATCACTCAGT TTGAGCTGTC AAAGGACCCT
    GACCCCATAC CTTTGAATCA CAACATGAGA TTTTATGCTG CTCTTCCAGG TCAGCAGCAC
    TGCTATTTCC TCAACAAGGA TGCTCCTCTT CCAGATGGCC GAAGTCTACA GGGAACCCTA
    GTTAGCAAAA TCACCTTTCA GCACCCTGGC CGAGTTCCTC TTATCCTAAA TCTGATCAGA
    CATCAAGTGG CCTATAACAC CCTAATAGGA AGTTGTGTCA AAAGAACTAT TCTGAAAGAA
    GATTCTCCTG GGCTCCTCCA ATTTGAAGTG TGTCCTCTCT CAGAGTCCCG TTTTAGCGTA
    TCTTTTCAGC ACCCTGTGAA TGACTCCCTG GTGTGTGTGG TAATGGATGT GCAGGACTCA
    ACACATGTGA GCTGTAAACT GTACAAGGGG CTGTCAGATG CACTCATCTG CACAGATGAC
    TTCATTGCCA AAGTTGTTCA AAGATGTATG TCCATCCCTG TGACGATGAG GGCTATTCGG
    AGGAAAGCTG AAACCATTCA GGCCGACACC CCAGCACTGT CCCTCATTGC AGAGACAGTT
    GAAGACATGG TGAAAAAGAA CCTGCCCCCG GCTAGCAGCC CAGGGTATGG CATGACCACA
    GGCAACAACC CAATGAGTGG TACCACTACA CCAACCAACA CCTTTCCGGG GGGTCCCATT
    ACCACCTTGT TTAATATGAG CATGAGCATC AAAGATCGGC ATGAGTCGGT GGGCCATGGG
    GAGGACTTCA GCAAGGTGTC TCAGAACCCA ATTCTTACCA GTTTGTTGCA AATCACAGGG
    AACGGGGGGT CTACCATTGG CTCGAGTCCG ACCCCTCCTC ATCACACGCC GCCACCTGTC
    TCTTCGATGG CCGGCAACAC CAAGAACCAC CCGATGCTCA TGAACCTTCT TAAAGATAAC
    CCTGCCCAGG ATTTCTCAAC CCTTTATGGA AGCAGCCCTT TAGAAAGGCA GAACTCTTCT
    TCCGGCTCAC CCCGGATGGA AATATGCCCG GGAAGCAACA AGACAAAGAA GAAGAAGTCA
    TCAAGATTAC CACCTGACAA ACCCAAGCAC CAGACTGAAG ATGACTTTCA GAGGGAGCTA
    TTTTCAATGG ATGTTGACTC ACAGAACCCT ATCTTTGATG TCAACATGAC AGCAGACACG
    CTGGATACGC CACACATCAC TCCAGCTCCA AGCCAGTGTA GTACTCCCCC AACAACTTAC
    CCGCAACCAG TACCTCACCC CCAACCCAGT ATTCAAAGGA TGGTCCGACT ATCCAGTTCA
    GACAGCATTG GCCCAGATGT AACTGATATC CTTTCAGACA TTGCAGAAGA AGCCTCTAAG
    CTTCCCAGCA CTAATGACGA TTGTCCACCC ATTGGCACCC CTGTTCGAGA TTCTTCAAGC
    TCTGGGCATT CTCAGAGTGC TCTCTTTGAC CCTGATGTCT TTCAAACCAA TAATAATGAA
    AATCCATACA CAGATCCAGC TGACCTAATT GCAGATGCTG CTGGTAGCCC TAGTAGTGAC
    TCTCCTACAA ATCATTTTTT TCCTGATGGA GTAGATTTTA ATCCTGATTT GTTGAACAGC
    CAGAGCCAAA GTGGTTTTGG AGAAGAATAT TTTGATGAAA GCAGCCAAAG CGGAGATAAT
    GATGATTTCA AAGGATTTGC ATCTCAGGCA CTAAATACTT TGGGGGTGCC AATGCTTGGA
    GGTGATAATG GGGAGACTAA GTTTAAAGGC AATAGCCAAG CTGACACAGT TGATTTCAGT
    ATTATAGCAG TGGCTGGTAA GGCTTTAGGT CCTGCAGATC TTATGGAGCA TCACAGTGGT
    AGCCAGAGTC CTTTATTGAC TACTGGGGAC TTAGGGAAAG AAAAGACTCA AAAACGAGTA
    AAGGAAGGCA ATGGCACCAG TAACAGTAGT CTGTCAGGGC CAGGATTAGA TAGCAAACCA
    GGGAAGCGTA GTCGGACCCC TTCTAATGAT GGCAAAAGCA AAGATAAGCC TCCAAAGCGG
    AAGAAGGCAG ACACTGAAGG AAAGTCTCCA TCTCACAGTT CTTCTAACCG ACCTTTCACT
    CCACCTACCA GTACGGGTGG ATCCAAATCA CCAGGCAGTT CAGGAAGATC ACAGACTCCC
    CCAGGTGTTG CCACACCACC TATTCCCAAA ATCACTATTC AGATTCCTAA AGGTACCGTG
    ATGGTGGGCA AGCCCTCATC CCACAGTCAG TATACCAGCA GTGGTTCTGT GTCTTCCTCA
    GGCAGTAAAA GCCACCATAG CCATTCTTCC TCCTCTTCTT CCTCCTCTTC TGCTTCCAAC
    TCAGGCAAGA TGAAAAGCAG TAAATCAGAA GGTTCATCAA GTTCCAAGTT AAGTAGCAGT
    GTATATTCTA GCCAAGGGTC TTCTGGATCT AGTCAGTCCA AAAATTCATC CCAGTCTGGG
    GGGAAGCCAG GCTCCTCTCC CATTACCAAG CATGGACTGA GCAGTGGCTC CGGCAGCACC
    AAGATGAAAC CTCAAGGGAA GCCATCATCA CTTATGAATC CTTCTTTAAG TAAACCAAAC
    ATATCCCCTT CTCATTCAAG GCCACCTGGA GGCTCTGATA AGCTTGCCTC TCCAATGAAG
    CCTGTTCCTG GGACTCCCCC ATCTTCTAAA GCCAAGTCCC CCATCAGTTC AGGTTCTGGT
    GGTTCTCATA TGTCTGGAAG TAGTTCAAGC ACTGGCATGA AGTCATCTTC AGGGTTAGGA
    TCTTCAGGCT CATTGTCTCA GAAAACTCCC CCATCATCTA ATTCTTGTAC AGCATCTTCC
    TCTTCCTTTT CCTCAAGTGG CTCTTCCATG TCATCCTCTC AGAACCAGCA CGGGAGTTCC
    AAAGGGAAAT CCCCCAGCAG AAATAAGAAG CCGTCCTTAA CAGCCGTCAT AGATAAACTG
    AAGCATGGGG TTGTCACCAG TGGCCCTGGA GGTGAAGACC CGATGGATGG CCAGATGGGA
    GTGAGCACAA ATTCTTCTAG CCATCCTATG TCCTCCAAAC ATAACATGTC AGGAGGAGAG
    TTCCAGGGTA AGCGTGAGAA AAGTGATAAA GAAAAATCCA AGGTTTCCAC CTCGGGGGGT
    TCAGTGGATT CGTCTAAGAA GACCTCAGAG TCAAAAAATG TGGGGAGCAC AGGTGTGGCA
    AAAATTATCA TCAGCAAGCA TGATGGGGGT TCCCCCAGCA TTAAAGCCAA AGTGACTTTG
    CAGAAACCTG GGGAAAGTAG TGGAGAAGGG CTCAGACCTC AGATGGCCTC TTCCAAAAAC
    TATGGCTCTC CACTCATCAG TGGTTCCACT CCAAAGCATG AGCGTGGCTC TCCTAGCCAT
    AGTAAGTCAC CAGCATATAC CCCCCAGAAT CTGGACAGTG AAAGTGAGTC AGGCTCCTCC
    ATAGCAGAGA AATCTTATCA GAACAGTCCG AGCTCAGATG ATGGTATCCG ACCTCTTCCA
    GAATATAGCA CAGAAAAGCA TAAGAAGCAC AAAAAAGAAA AGAAGAAAGT AAAAGACAAA
    GATAGAGACC GGGACCGGGA CAAAGACCGG GACAAGAAAA AATCTCATAG CATCAAGCCA
    GAGAGTTGGT CTAAATCACC CATCTCTTCA GACCAGTCCT TGTCTATGAC AAGCAACACA
    ATTTTATCTA CAGACAGGCC CTCAAGGCTC AGCCCTGACT TTATGATTGG GGAGGAAGAT
    GATGATCTTA TGGATGTGGC CCTGATTGGC AATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013972078.1
    CDS234..4469
    Translation

    Target ORF information:

    RefSeq Version XM_014116603.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris mediator complex subunit 1 (MED1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014116603.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTACTTGG CTCTCCAATC CTTAGAACAG GATCTATCTA AAATGGCAGT TATGTATTGG 
    AAAGCAACCA ATGCTGGTCC CTTGGATAAG ATTCTTCATG GAAGTGTTGG CTATCTTACT
    CCAAGAAGTG GGGGTCATTT AATGAACTTG AAGTACTATG CCTCTCCTTC TGACCTGCTG
    GATGATAAGA CTACATCTCC CATCATTTTG CATGAGAATA ATGTTCCTCG ATCATTGGGC
    ATGAACGCAT CAGTGACAAT TGAAGGAACA TCTGCTATGT ATAAACTCCC AATTGCACCA
    TTAATTATGG GGTCACATCC AGTTGACAAC AAATGGACCC CCTCCTTCTC CTCAATCACC
    AGTGCCAACA GTGTTGATCT TCCTGCCTGT TTCTTCTTGA AATTTCCCCA ACCAATCCCA
    GTATCTAGAG CATTTGTTCA GAAACTTCAG AACTGCACAG GAATTCCATT GTTTGAAACT
    CAACCAACTT ATGTACCCCT CTATGAACTG ATCACTCAGT TTGAGCTGTC AAAGGACCCT
    GACCCCATAC CTTTGAATCA CAACATGAGA TTTTATGCTG CTCTTCCAGG TCAGCAGCAC
    TGCTATTTCC TCAACAAGGA TGCTCCTCTT CCAGATGGCC GAAGTCTACA GGGAACCCTA
    GTTAGCAAAA TCACCTTTCA GCACCCTGGC CGAGTTCCTC TTATCCTAAA TCTGATCAGA
    CATCAAGTGG CCTATAACAC CCTAATAGGA AGTTGTGTCA AAAGAACTAT TCTGAAAGAA
    GATTCTCCTG GGCTCCTCCA ATTTGAAGTG TGTCCTCTCT CAGAGTCCCG TTTTAGCGTA
    TCTTTTCAGC ACCCTGTGAA TGACTCCCTG GTGTGTGTGG TAATGGATGT GCAGGACTCA
    ACACATGTGA GCTGTAAACT GTACAAGGGG CTGTCAGATG CACTCATCTG CACAGATGAC
    TTCATTGCCA AAGTTGTTCA AAGATGTATG TCCATCCCTG TGACGATGAG GGCTATTCGG
    AGGAAAGCTG AAACCATTCA GGCCGACACC CCAGCACTGT CCCTCATTGC AGAGACAGTT
    GAAGACATGG TGAAAAAGAA CCTGCCCCCG GCTAGCAGCC CAGGGTATGG CATGACCACA
    GGCAACAACC CAATGAGTGG TACCACTACA CCAACCAACA CCTTTCCGGG GGGTCCCATT
    ACCACCTTGT TTAATATGAG CATGAGCATC AAAGATCGGC ATGAGTCGGT GGGCCATGGG
    GAGGACTTCA GCAAGGTGTC TCAGAACCCA ATTCTTACCA GTTTGTTGCA AATCACAGGG
    AACGGGGGGT CTACCATTGG CTCGAGTCCG ACCCCTCCTC ATCACACGCC GCCACCTGTC
    TCTTCGATGG CCGGCAACAC CAAGAACCAC CCGATGCTCA TGAACCTTCT TAAAGATAAC
    CCTGCCCAGG ATTTCTCAAC CCTTTATGGA AGCAGCCCTT TAGAAAGGCA GAACTCTTCT
    TCCGGCTCAC CCCGGATGGA AATATGCCCG GGAAGCAACA AGACAAAGAA GAAGAAGTCA
    TCAAGATTAC CACCTGACAA ACCCAAGCAC CAGACTGAAG ATGACTTTCA GAGGGAGCTA
    TTTTCAATGG ATGTTGACTC ACAGAACCCT ATCTTTGATG TCAACATGAC AGCAGACACG
    CTGGATACGC CACACATCAC TCCAGCTCCA AGCCAGTGTA GTACTCCCCC AACAACTTAC
    CCGCAACCAG TACCTCACCC CCAACCCAGT ATTCAAAGGA TGGTCCGACT ATCCAGTTCA
    GACAGCATTG GCCCAGATGT AACTGATATC CTTTCAGACA TTGCAGAAGA AGCCTCTAAG
    CTTCCCAGCA CTAATGACGA TTGTCCACCC ATTGGCACCC CTGTTCGAGA TTCTTCAAGC
    TCTGGGCATT CTCAGAGTGC TCTCTTTGAC CCTGATGTCT TTCAAACCAA TAATAATGAA
    AATCCATACA CAGATCCAGC TGACCTAATT GCAGATGCTG CTGGTAGCCC TAGTAGTGAC
    TCTCCTACAA ATCATTTTTT TCCTGATGGA GTAGATTTTA ATCCTGATTT GTTGAACAGC
    CAGAGCCAAA GTGGTTTTGG AGAAGAATAT TTTGATGAAA GCAGCCAAAG CGGAGATAAT
    GATGATTTCA AAGGATTTGC ATCTCAGGCA CTAAATACTT TGGGGGTGCC AATGCTTGGA
    GGTGATAATG GGGAGACTAA GTTTAAAGGC AATAGCCAAG CTGACACAGT TGATTTCAGT
    ATTATAGCAG TGGCTGGTAA GGCTTTAGGT CCTGCAGATC TTATGGAGCA TCACAGTGGT
    AGCCAGAGTC CTTTATTGAC TACTGGGGAC TTAGGGAAAG AAAAGACTCA AAAACGAGTA
    AAGGAAGGCA ATGGCACCAG TAACAGTAGT CTGTCAGGGC CAGGATTAGA TAGCAAACCA
    GGGAAGCGTA GTCGGACCCC TTCTAATGAT GGCAAAAGCA AAGATAAGCC TCCAAAGCGG
    AAGAAGGCAG ACACTGAAGG AAAGTCTCCA TCTCACAGTT CTTCTAACCG ACCTTTCACT
    CCACCTACCA GTACGGGTGG ATCCAAATCA CCAGGCAGTT CAGGAAGATC ACAGACTCCC
    CCAGGTGTTG CCACACCACC TATTCCCAAA ATCACTATTC AGATTCCTAA AGGTACCGTG
    ATGGTGGGCA AGCCCTCATC CCACAGTCAG TATACCAGCA GTGGTTCTGT GTCTTCCTCA
    GGCAGTAAAA GCCACCATAG CCATTCTTCC TCCTCTTCTT CCTCCTCTTC TGCTTCCAAC
    TCAGGCAAGA TGAAAAGCAG TAAATCAGAA GGTTCATCAA GTTCCAAGTT AAGTAGCAGT
    GTATATTCTA GCCAAGGGTC TTCTGGATCT AGTCAGTCCA AAAATTCATC CCAGTCTGGG
    GGGAAGCCAG GCTCCTCTCC CATTACCAAG CATGGACTGA GCAGTGGCTC CGGCAGCACC
    AAGATGAAAC CTCAAGGGAA GCCATCATCA CTTATGAATC CTTCTTTAAG TAAACCAAAC
    ATATCCCCTT CTCATTCAAG GCCACCTGGA GGCTCTGATA AGCTTGCCTC TCCAATGAAG
    CCTGTTCCTG GGACTCCCCC ATCTTCTAAA GCCAAGTCCC CCATCAGTTC AGGTTCTGGT
    GGTTCTCATA TGTCTGGAAG TAGTTCAAGC ACTGGCATGA AGTCATCTTC AGGGTTAGGA
    TCTTCAGGCT CATTGTCTCA GAAAACTCCC CCATCATCTA ATTCTTGTAC AGCATCTTCC
    TCTTCCTTTT CCTCAAGTGG CTCTTCCATG TCATCCTCTC AGAACCAGCA CGGGAGTTCC
    AAAGGGAAAT CCCCCAGCAG AAATAAGAAG CCGTCCTTAA CAGCCGTCAT AGATAAACTG
    AAGCATGGGG TTGTCACCAG TGGCCCTGGA GGTGAAGACC CGATGGATGG CCAGATGGGA
    GTGAGCACAA ATTCTTCTAG CCATCCTATG TCCTCCAAAC ATAACATGTC AGGAGGAGAG
    TTCCAGGGTA AGCGTGAGAA AAGTGATAAA GAAAAATCCA AGGTTTCCAC CTCGGGGGGT
    TCAGTGGATT CGTCTAAGAA GACCTCAGAG TCAAAAAATG TGGGGAGCAC AGGTGTGGCA
    AAAATTATCA TCAGCAAGCA TGATGGGGGT TCCCCCAGCA TTAAAGCCAA AGTGACTTTG
    CAGAAACCTG GGGAAAGTAG TGGAGAAGGG CTCAGACCTC AGATGGCCTC TTCCAAAAAC
    TATGGCTCTC CACTCATCAG TGGTTCCACT CCAAAGCATG AGCGTGGCTC TCCTAGCCAT
    AGTAAGTCAC CAGCATATAC CCCCCAGAAT CTGGACAGTG AAAGTGAGTC AGGCTCCTCC
    ATAGCAGAGA AATCTTATCA GAACAGTCCG AGCTCAGATG ATGGTATCCG ACCTCTTCCA
    GAATATAGCA CAGAAAAGCA TAAGAAGCAC AAAAAAGAAA AGAAGAAAGT AAAAGACAAA
    GATAGAGACC GGGACCGGGA CAAAGACCGG GACAAGAAAA AATCTCATAG CATCAAGCCA
    GAGAGTTGGT CTAAATCACC CATCTCTTCA GACCAGTCCT TGTCTATGAC AAGCAACACA
    ATTTTATCTA CAGACAGGCC CTCAAGGCTC AGCCCTGACT TTATGATTGG GGAGGAAGAT
    GATGATCTTA TGGATGTGGC CCTGATTGGC AATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe38367
    Clone ID Related Accession (Same CDS sequence) XM_014116603.2 , XM_022423023.1 , XM_014116603.1
    Accession Version XM_022423023.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4236bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product mediator of RNA polymerase II transcription subunit 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTACTTGG CTCTCCAATC CTTAGAACAG GATCTATCTA AAATGGCAGT TATGTATTGG 
    AAAGCAACCA ATGCTGGTCC CTTGGATAAG ATTCTTCATG GAAGTGTTGG CTATCTTACT
    CCAAGAAGTG GGGGTCATTT AATGAACTTG AAGTACTATG CCTCTCCTTC TGACCTGCTG
    GATGATAAGA CTACATCTCC CATCATTTTG CATGAGAATA ATGTTCCTCG ATCATTGGGC
    ATGAACGCAT CAGTGACAAT TGAAGGAACA TCTGCTATGT ATAAACTCCC AATTGCACCA
    TTAATTATGG GGTCACATCC AGTTGACAAC AAATGGACCC CCTCCTTCTC CTCAATCACC
    AGTGCCAACA GTGTTGATCT TCCTGCCTGT TTCTTCTTGA AATTTCCCCA ACCAATCCCA
    GTATCTAGAG CATTTGTTCA GAAACTTCAG AACTGCACAG GAATTCCATT GTTTGAAACT
    CAACCAACTT ATGTACCCCT CTATGAACTG ATCACTCAGT TTGAGCTGTC AAAGGACCCT
    GACCCCATAC CTTTGAATCA CAACATGAGA TTTTATGCTG CTCTTCCAGG TCAGCAGCAC
    TGCTATTTCC TCAACAAGGA TGCTCCTCTT CCAGATGGCC GAAGTCTACA GGGAACCCTA
    GTTAGCAAAA TCACCTTTCA GCACCCTGGC CGAGTTCCTC TTATCCTAAA TCTGATCAGA
    CATCAAGTGG CCTATAACAC CCTAATAGGA AGTTGTGTCA AAAGAACTAT TCTGAAAGAA
    GATTCTCCTG GGCTCCTCCA ATTTGAAGTG TGTCCTCTCT CAGAGTCCCG TTTTAGCGTA
    TCTTTTCAGC ACCCTGTGAA TGACTCCCTG GTGTGTGTGG TAATGGATGT GCAGGACTCA
    ACACATGTGA GCTGTAAACT GTACAAGGGG CTGTCAGATG CACTCATCTG CACAGATGAC
    TTCATTGCCA AAGTTGTTCA AAGATGTATG TCCATCCCTG TGACGATGAG GGCTATTCGG
    AGGAAAGCTG AAACCATTCA GGCCGACACC CCAGCACTGT CCCTCATTGC AGAGACAGTT
    GAAGACATGG TGAAAAAGAA CCTGCCCCCG GCTAGCAGCC CAGGGTATGG CATGACCACA
    GGCAACAACC CAATGAGTGG TACCACTACA CCAACCAACA CCTTTCCGGG GGGTCCCATT
    ACCACCTTGT TTAATATGAG CATGAGCATC AAAGATCGGC ATGAGTCGGT GGGCCATGGG
    GAGGACTTCA GCAAGGTGTC TCAGAACCCA ATTCTTACCA GTTTGTTGCA AATCACAGGG
    AACGGGGGGT CTACCATTGG CTCGAGTCCG ACCCCTCCTC ATCACACGCC GCCACCTGTC
    TCTTCGATGG CCGGCAACAC CAAGAACCAC CCGATGCTCA TGAACCTTCT TAAAGATAAC
    CCTGCCCAGG ATTTCTCAAC CCTTTATGGA AGCAGCCCTT TAGAAAGGCA GAACTCTTCT
    TCCGGCTCAC CCCGGATGGA AATATGCCCG GGAAGCAACA AGACAAAGAA GAAGAAGTCA
    TCAAGATTAC CACCTGACAA ACCCAAGCAC CAGACTGAAG ATGACTTTCA GAGGGAGCTA
    TTTTCAATGG ATGTTGACTC ACAGAACCCT ATCTTTGATG TCAACATGAC AGCAGACACG
    CTGGATACGC CACACATCAC TCCAGCTCCA AGCCAGTGTA GTACTCCCCC AACAACTTAC
    CCGCAACCAG TACCTCACCC CCAACCCAGT ATTCAAAGGA TGGTCCGACT ATCCAGTTCA
    GACAGCATTG GCCCAGATGT AACTGATATC CTTTCAGACA TTGCAGAAGA AGCCTCTAAG
    CTTCCCAGCA CTAATGACGA TTGTCCACCC ATTGGCACCC CTGTTCGAGA TTCTTCAAGC
    TCTGGGCATT CTCAGAGTGC TCTCTTTGAC CCTGATGTCT TTCAAACCAA TAATAATGAA
    AATCCATACA CAGATCCAGC TGACCTAATT GCAGATGCTG CTGGTAGCCC TAGTAGTGAC
    TCTCCTACAA ATCATTTTTT TCCTGATGGA GTAGATTTTA ATCCTGATTT GTTGAACAGC
    CAGAGCCAAA GTGGTTTTGG AGAAGAATAT TTTGATGAAA GCAGCCAAAG CGGAGATAAT
    GATGATTTCA AAGGATTTGC ATCTCAGGCA CTAAATACTT TGGGGGTGCC AATGCTTGGA
    GGTGATAATG GGGAGACTAA GTTTAAAGGC AATAGCCAAG CTGACACAGT TGATTTCAGT
    ATTATAGCAG TGGCTGGTAA GGCTTTAGGT CCTGCAGATC TTATGGAGCA TCACAGTGGT
    AGCCAGAGTC CTTTATTGAC TACTGGGGAC TTAGGGAAAG AAAAGACTCA AAAACGAGTA
    AAGGAAGGCA ATGGCACCAG TAACAGTAGT CTGTCAGGGC CAGGATTAGA TAGCAAACCA
    GGGAAGCGTA GTCGGACCCC TTCTAATGAT GGCAAAAGCA AAGATAAGCC TCCAAAGCGG
    AAGAAGGCAG ACACTGAAGG AAAGTCTCCA TCTCACAGTT CTTCTAACCG ACCTTTCACT
    CCACCTACCA GTACGGGTGG ATCCAAATCA CCAGGCAGTT CAGGAAGATC ACAGACTCCC
    CCAGGTGTTG CCACACCACC TATTCCCAAA ATCACTATTC AGATTCCTAA AGGTACCGTG
    ATGGTGGGCA AGCCCTCATC CCACAGTCAG TATACCAGCA GTGGTTCTGT GTCTTCCTCA
    GGCAGTAAAA GCCACCATAG CCATTCTTCC TCCTCTTCTT CCTCCTCTTC TGCTTCCAAC
    TCAGGCAAGA TGAAAAGCAG TAAATCAGAA GGTTCATCAA GTTCCAAGTT AAGTAGCAGT
    GTATATTCTA GCCAAGGGTC TTCTGGATCT AGTCAGTCCA AAAATTCATC CCAGTCTGGG
    GGGAAGCCAG GCTCCTCTCC CATTACCAAG CATGGACTGA GCAGTGGCTC CGGCAGCACC
    AAGATGAAAC CTCAAGGGAA GCCATCATCA CTTATGAATC CTTCTTTAAG TAAACCAAAC
    ATATCCCCTT CTCATTCAAG GCCACCTGGA GGCTCTGATA AGCTTGCCTC TCCAATGAAG
    CCTGTTCCTG GGACTCCCCC ATCTTCTAAA GCCAAGTCCC CCATCAGTTC AGGTTCTGGT
    GGTTCTCATA TGTCTGGAAG TAGTTCAAGC ACTGGCATGA AGTCATCTTC AGGGTTAGGA
    TCTTCAGGCT CATTGTCTCA GAAAACTCCC CCATCATCTA ATTCTTGTAC AGCATCTTCC
    TCTTCCTTTT CCTCAAGTGG CTCTTCCATG TCATCCTCTC AGAACCAGCA CGGGAGTTCC
    AAAGGGAAAT CCCCCAGCAG AAATAAGAAG CCGTCCTTAA CAGCCGTCAT AGATAAACTG
    AAGCATGGGG TTGTCACCAG TGGCCCTGGA GGTGAAGACC CGATGGATGG CCAGATGGGA
    GTGAGCACAA ATTCTTCTAG CCATCCTATG TCCTCCAAAC ATAACATGTC AGGAGGAGAG
    TTCCAGGGTA AGCGTGAGAA AAGTGATAAA GAAAAATCCA AGGTTTCCAC CTCGGGGGGT
    TCAGTGGATT CGTCTAAGAA GACCTCAGAG TCAAAAAATG TGGGGAGCAC AGGTGTGGCA
    AAAATTATCA TCAGCAAGCA TGATGGGGGT TCCCCCAGCA TTAAAGCCAA AGTGACTTTG
    CAGAAACCTG GGGAAAGTAG TGGAGAAGGG CTCAGACCTC AGATGGCCTC TTCCAAAAAC
    TATGGCTCTC CACTCATCAG TGGTTCCACT CCAAAGCATG AGCGTGGCTC TCCTAGCCAT
    AGTAAGTCAC CAGCATATAC CCCCCAGAAT CTGGACAGTG AAAGTGAGTC AGGCTCCTCC
    ATAGCAGAGA AATCTTATCA GAACAGTCCG AGCTCAGATG ATGGTATCCG ACCTCTTCCA
    GAATATAGCA CAGAAAAGCA TAAGAAGCAC AAAAAAGAAA AGAAGAAAGT AAAAGACAAA
    GATAGAGACC GGGACCGGGA CAAAGACCGG GACAAGAAAA AATCTCATAG CATCAAGCCA
    GAGAGTTGGT CTAAATCACC CATCTCTTCA GACCAGTCCT TGTCTATGAC AAGCAACACA
    ATTTTATCTA CAGACAGGCC CTCAAGGCTC AGCCCTGACT TTATGATTGG GGAGGAAGAT
    GATGATCTTA TGGATGTGGC CCTGATTGGC AATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022278731.1
    CDS634..4869
    Translation

    Target ORF information:

    RefSeq Version XM_022423023.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris mediator complex subunit 1 (MED1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022423023.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTACTTGG CTCTCCAATC CTTAGAACAG GATCTATCTA AAATGGCAGT TATGTATTGG 
    AAAGCAACCA ATGCTGGTCC CTTGGATAAG ATTCTTCATG GAAGTGTTGG CTATCTTACT
    CCAAGAAGTG GGGGTCATTT AATGAACTTG AAGTACTATG CCTCTCCTTC TGACCTGCTG
    GATGATAAGA CTACATCTCC CATCATTTTG CATGAGAATA ATGTTCCTCG ATCATTGGGC
    ATGAACGCAT CAGTGACAAT TGAAGGAACA TCTGCTATGT ATAAACTCCC AATTGCACCA
    TTAATTATGG GGTCACATCC AGTTGACAAC AAATGGACCC CCTCCTTCTC CTCAATCACC
    AGTGCCAACA GTGTTGATCT TCCTGCCTGT TTCTTCTTGA AATTTCCCCA ACCAATCCCA
    GTATCTAGAG CATTTGTTCA GAAACTTCAG AACTGCACAG GAATTCCATT GTTTGAAACT
    CAACCAACTT ATGTACCCCT CTATGAACTG ATCACTCAGT TTGAGCTGTC AAAGGACCCT
    GACCCCATAC CTTTGAATCA CAACATGAGA TTTTATGCTG CTCTTCCAGG TCAGCAGCAC
    TGCTATTTCC TCAACAAGGA TGCTCCTCTT CCAGATGGCC GAAGTCTACA GGGAACCCTA
    GTTAGCAAAA TCACCTTTCA GCACCCTGGC CGAGTTCCTC TTATCCTAAA TCTGATCAGA
    CATCAAGTGG CCTATAACAC CCTAATAGGA AGTTGTGTCA AAAGAACTAT TCTGAAAGAA
    GATTCTCCTG GGCTCCTCCA ATTTGAAGTG TGTCCTCTCT CAGAGTCCCG TTTTAGCGTA
    TCTTTTCAGC ACCCTGTGAA TGACTCCCTG GTGTGTGTGG TAATGGATGT GCAGGACTCA
    ACACATGTGA GCTGTAAACT GTACAAGGGG CTGTCAGATG CACTCATCTG CACAGATGAC
    TTCATTGCCA AAGTTGTTCA AAGATGTATG TCCATCCCTG TGACGATGAG GGCTATTCGG
    AGGAAAGCTG AAACCATTCA GGCCGACACC CCAGCACTGT CCCTCATTGC AGAGACAGTT
    GAAGACATGG TGAAAAAGAA CCTGCCCCCG GCTAGCAGCC CAGGGTATGG CATGACCACA
    GGCAACAACC CAATGAGTGG TACCACTACA CCAACCAACA CCTTTCCGGG GGGTCCCATT
    ACCACCTTGT TTAATATGAG CATGAGCATC AAAGATCGGC ATGAGTCGGT GGGCCATGGG
    GAGGACTTCA GCAAGGTGTC TCAGAACCCA ATTCTTACCA GTTTGTTGCA AATCACAGGG
    AACGGGGGGT CTACCATTGG CTCGAGTCCG ACCCCTCCTC ATCACACGCC GCCACCTGTC
    TCTTCGATGG CCGGCAACAC CAAGAACCAC CCGATGCTCA TGAACCTTCT TAAAGATAAC
    CCTGCCCAGG ATTTCTCAAC CCTTTATGGA AGCAGCCCTT TAGAAAGGCA GAACTCTTCT
    TCCGGCTCAC CCCGGATGGA AATATGCCCG GGAAGCAACA AGACAAAGAA GAAGAAGTCA
    TCAAGATTAC CACCTGACAA ACCCAAGCAC CAGACTGAAG ATGACTTTCA GAGGGAGCTA
    TTTTCAATGG ATGTTGACTC ACAGAACCCT ATCTTTGATG TCAACATGAC AGCAGACACG
    CTGGATACGC CACACATCAC TCCAGCTCCA AGCCAGTGTA GTACTCCCCC AACAACTTAC
    CCGCAACCAG TACCTCACCC CCAACCCAGT ATTCAAAGGA TGGTCCGACT ATCCAGTTCA
    GACAGCATTG GCCCAGATGT AACTGATATC CTTTCAGACA TTGCAGAAGA AGCCTCTAAG
    CTTCCCAGCA CTAATGACGA TTGTCCACCC ATTGGCACCC CTGTTCGAGA TTCTTCAAGC
    TCTGGGCATT CTCAGAGTGC TCTCTTTGAC CCTGATGTCT TTCAAACCAA TAATAATGAA
    AATCCATACA CAGATCCAGC TGACCTAATT GCAGATGCTG CTGGTAGCCC TAGTAGTGAC
    TCTCCTACAA ATCATTTTTT TCCTGATGGA GTAGATTTTA ATCCTGATTT GTTGAACAGC
    CAGAGCCAAA GTGGTTTTGG AGAAGAATAT TTTGATGAAA GCAGCCAAAG CGGAGATAAT
    GATGATTTCA AAGGATTTGC ATCTCAGGCA CTAAATACTT TGGGGGTGCC AATGCTTGGA
    GGTGATAATG GGGAGACTAA GTTTAAAGGC AATAGCCAAG CTGACACAGT TGATTTCAGT
    ATTATAGCAG TGGCTGGTAA GGCTTTAGGT CCTGCAGATC TTATGGAGCA TCACAGTGGT
    AGCCAGAGTC CTTTATTGAC TACTGGGGAC TTAGGGAAAG AAAAGACTCA AAAACGAGTA
    AAGGAAGGCA ATGGCACCAG TAACAGTAGT CTGTCAGGGC CAGGATTAGA TAGCAAACCA
    GGGAAGCGTA GTCGGACCCC TTCTAATGAT GGCAAAAGCA AAGATAAGCC TCCAAAGCGG
    AAGAAGGCAG ACACTGAAGG AAAGTCTCCA TCTCACAGTT CTTCTAACCG ACCTTTCACT
    CCACCTACCA GTACGGGTGG ATCCAAATCA CCAGGCAGTT CAGGAAGATC ACAGACTCCC
    CCAGGTGTTG CCACACCACC TATTCCCAAA ATCACTATTC AGATTCCTAA AGGTACCGTG
    ATGGTGGGCA AGCCCTCATC CCACAGTCAG TATACCAGCA GTGGTTCTGT GTCTTCCTCA
    GGCAGTAAAA GCCACCATAG CCATTCTTCC TCCTCTTCTT CCTCCTCTTC TGCTTCCAAC
    TCAGGCAAGA TGAAAAGCAG TAAATCAGAA GGTTCATCAA GTTCCAAGTT AAGTAGCAGT
    GTATATTCTA GCCAAGGGTC TTCTGGATCT AGTCAGTCCA AAAATTCATC CCAGTCTGGG
    GGGAAGCCAG GCTCCTCTCC CATTACCAAG CATGGACTGA GCAGTGGCTC CGGCAGCACC
    AAGATGAAAC CTCAAGGGAA GCCATCATCA CTTATGAATC CTTCTTTAAG TAAACCAAAC
    ATATCCCCTT CTCATTCAAG GCCACCTGGA GGCTCTGATA AGCTTGCCTC TCCAATGAAG
    CCTGTTCCTG GGACTCCCCC ATCTTCTAAA GCCAAGTCCC CCATCAGTTC AGGTTCTGGT
    GGTTCTCATA TGTCTGGAAG TAGTTCAAGC ACTGGCATGA AGTCATCTTC AGGGTTAGGA
    TCTTCAGGCT CATTGTCTCA GAAAACTCCC CCATCATCTA ATTCTTGTAC AGCATCTTCC
    TCTTCCTTTT CCTCAAGTGG CTCTTCCATG TCATCCTCTC AGAACCAGCA CGGGAGTTCC
    AAAGGGAAAT CCCCCAGCAG AAATAAGAAG CCGTCCTTAA CAGCCGTCAT AGATAAACTG
    AAGCATGGGG TTGTCACCAG TGGCCCTGGA GGTGAAGACC CGATGGATGG CCAGATGGGA
    GTGAGCACAA ATTCTTCTAG CCATCCTATG TCCTCCAAAC ATAACATGTC AGGAGGAGAG
    TTCCAGGGTA AGCGTGAGAA AAGTGATAAA GAAAAATCCA AGGTTTCCAC CTCGGGGGGT
    TCAGTGGATT CGTCTAAGAA GACCTCAGAG TCAAAAAATG TGGGGAGCAC AGGTGTGGCA
    AAAATTATCA TCAGCAAGCA TGATGGGGGT TCCCCCAGCA TTAAAGCCAA AGTGACTTTG
    CAGAAACCTG GGGAAAGTAG TGGAGAAGGG CTCAGACCTC AGATGGCCTC TTCCAAAAAC
    TATGGCTCTC CACTCATCAG TGGTTCCACT CCAAAGCATG AGCGTGGCTC TCCTAGCCAT
    AGTAAGTCAC CAGCATATAC CCCCCAGAAT CTGGACAGTG AAAGTGAGTC AGGCTCCTCC
    ATAGCAGAGA AATCTTATCA GAACAGTCCG AGCTCAGATG ATGGTATCCG ACCTCTTCCA
    GAATATAGCA CAGAAAAGCA TAAGAAGCAC AAAAAAGAAA AGAAGAAAGT AAAAGACAAA
    GATAGAGACC GGGACCGGGA CAAAGACCGG GACAAGAAAA AATCTCATAG CATCAAGCCA
    GAGAGTTGGT CTAAATCACC CATCTCTTCA GACCAGTCCT TGTCTATGAC AAGCAACACA
    ATTTTATCTA CAGACAGGCC CTCAAGGCTC AGCCCTGACT TTATGATTGG GGAGGAAGAT
    GATGATCTTA TGGATGTGGC CCTGATTGGC AATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe38367
    Clone ID Related Accession (Same CDS sequence) XM_014116603.2 , XM_022423023.1 , XM_014116603.1
    Accession Version XM_014116603.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4236bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product mediator of RNA polymerase II transcription subunit 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTACTTGG CTCTCCAATC CTTAGAACAG GATCTATCTA AAATGGCAGT TATGTATTGG 
    AAAGCAACCA ATGCTGGTCC CTTGGATAAG ATTCTTCATG GAAGTGTTGG CTATCTTACT
    CCAAGAAGTG GGGGTCATTT AATGAACTTG AAGTACTATG CCTCTCCTTC TGACCTGCTG
    GATGATAAGA CTACATCTCC CATCATTTTG CATGAGAATA ATGTTCCTCG ATCATTGGGC
    ATGAACGCAT CAGTGACAAT TGAAGGAACA TCTGCTATGT ATAAACTCCC AATTGCACCA
    TTAATTATGG GGTCACATCC AGTTGACAAC AAATGGACCC CCTCCTTCTC CTCAATCACC
    AGTGCCAACA GTGTTGATCT TCCTGCCTGT TTCTTCTTGA AATTTCCCCA ACCAATCCCA
    GTATCTAGAG CATTTGTTCA GAAACTTCAG AACTGCACAG GAATTCCATT GTTTGAAACT
    CAACCAACTT ATGTACCCCT CTATGAACTG ATCACTCAGT TTGAGCTGTC AAAGGACCCT
    GACCCCATAC CTTTGAATCA CAACATGAGA TTTTATGCTG CTCTTCCAGG TCAGCAGCAC
    TGCTATTTCC TCAACAAGGA TGCTCCTCTT CCAGATGGCC GAAGTCTACA GGGAACCCTA
    GTTAGCAAAA TCACCTTTCA GCACCCTGGC CGAGTTCCTC TTATCCTAAA TCTGATCAGA
    CATCAAGTGG CCTATAACAC CCTAATAGGA AGTTGTGTCA AAAGAACTAT TCTGAAAGAA
    GATTCTCCTG GGCTCCTCCA ATTTGAAGTG TGTCCTCTCT CAGAGTCCCG TTTTAGCGTA
    TCTTTTCAGC ACCCTGTGAA TGACTCCCTG GTGTGTGTGG TAATGGATGT GCAGGACTCA
    ACACATGTGA GCTGTAAACT GTACAAGGGG CTGTCAGATG CACTCATCTG CACAGATGAC
    TTCATTGCCA AAGTTGTTCA AAGATGTATG TCCATCCCTG TGACGATGAG GGCTATTCGG
    AGGAAAGCTG AAACCATTCA GGCCGACACC CCAGCACTGT CCCTCATTGC AGAGACAGTT
    GAAGACATGG TGAAAAAGAA CCTGCCCCCG GCTAGCAGCC CAGGGTATGG CATGACCACA
    GGCAACAACC CAATGAGTGG TACCACTACA CCAACCAACA CCTTTCCGGG GGGTCCCATT
    ACCACCTTGT TTAATATGAG CATGAGCATC AAAGATCGGC ATGAGTCGGT GGGCCATGGG
    GAGGACTTCA GCAAGGTGTC TCAGAACCCA ATTCTTACCA GTTTGTTGCA AATCACAGGG
    AACGGGGGGT CTACCATTGG CTCGAGTCCG ACCCCTCCTC ATCACACGCC GCCACCTGTC
    TCTTCGATGG CCGGCAACAC CAAGAACCAC CCGATGCTCA TGAACCTTCT TAAAGATAAC
    CCTGCCCAGG ATTTCTCAAC CCTTTATGGA AGCAGCCCTT TAGAAAGGCA GAACTCTTCT
    TCCGGCTCAC CCCGGATGGA AATATGCCCG GGAAGCAACA AGACAAAGAA GAAGAAGTCA
    TCAAGATTAC CACCTGACAA ACCCAAGCAC CAGACTGAAG ATGACTTTCA GAGGGAGCTA
    TTTTCAATGG ATGTTGACTC ACAGAACCCT ATCTTTGATG TCAACATGAC AGCAGACACG
    CTGGATACGC CACACATCAC TCCAGCTCCA AGCCAGTGTA GTACTCCCCC AACAACTTAC
    CCGCAACCAG TACCTCACCC CCAACCCAGT ATTCAAAGGA TGGTCCGACT ATCCAGTTCA
    GACAGCATTG GCCCAGATGT AACTGATATC CTTTCAGACA TTGCAGAAGA AGCCTCTAAG
    CTTCCCAGCA CTAATGACGA TTGTCCACCC ATTGGCACCC CTGTTCGAGA TTCTTCAAGC
    TCTGGGCATT CTCAGAGTGC TCTCTTTGAC CCTGATGTCT TTCAAACCAA TAATAATGAA
    AATCCATACA CAGATCCAGC TGACCTAATT GCAGATGCTG CTGGTAGCCC TAGTAGTGAC
    TCTCCTACAA ATCATTTTTT TCCTGATGGA GTAGATTTTA ATCCTGATTT GTTGAACAGC
    CAGAGCCAAA GTGGTTTTGG AGAAGAATAT TTTGATGAAA GCAGCCAAAG CGGAGATAAT
    GATGATTTCA AAGGATTTGC ATCTCAGGCA CTAAATACTT TGGGGGTGCC AATGCTTGGA
    GGTGATAATG GGGAGACTAA GTTTAAAGGC AATAGCCAAG CTGACACAGT TGATTTCAGT
    ATTATAGCAG TGGCTGGTAA GGCTTTAGGT CCTGCAGATC TTATGGAGCA TCACAGTGGT
    AGCCAGAGTC CTTTATTGAC TACTGGGGAC TTAGGGAAAG AAAAGACTCA AAAACGAGTA
    AAGGAAGGCA ATGGCACCAG TAACAGTAGT CTGTCAGGGC CAGGATTAGA TAGCAAACCA
    GGGAAGCGTA GTCGGACCCC TTCTAATGAT GGCAAAAGCA AAGATAAGCC TCCAAAGCGG
    AAGAAGGCAG ACACTGAAGG AAAGTCTCCA TCTCACAGTT CTTCTAACCG ACCTTTCACT
    CCACCTACCA GTACGGGTGG ATCCAAATCA CCAGGCAGTT CAGGAAGATC ACAGACTCCC
    CCAGGTGTTG CCACACCACC TATTCCCAAA ATCACTATTC AGATTCCTAA AGGTACCGTG
    ATGGTGGGCA AGCCCTCATC CCACAGTCAG TATACCAGCA GTGGTTCTGT GTCTTCCTCA
    GGCAGTAAAA GCCACCATAG CCATTCTTCC TCCTCTTCTT CCTCCTCTTC TGCTTCCAAC
    TCAGGCAAGA TGAAAAGCAG TAAATCAGAA GGTTCATCAA GTTCCAAGTT AAGTAGCAGT
    GTATATTCTA GCCAAGGGTC TTCTGGATCT AGTCAGTCCA AAAATTCATC CCAGTCTGGG
    GGGAAGCCAG GCTCCTCTCC CATTACCAAG CATGGACTGA GCAGTGGCTC CGGCAGCACC
    AAGATGAAAC CTCAAGGGAA GCCATCATCA CTTATGAATC CTTCTTTAAG TAAACCAAAC
    ATATCCCCTT CTCATTCAAG GCCACCTGGA GGCTCTGATA AGCTTGCCTC TCCAATGAAG
    CCTGTTCCTG GGACTCCCCC ATCTTCTAAA GCCAAGTCCC CCATCAGTTC AGGTTCTGGT
    GGTTCTCATA TGTCTGGAAG TAGTTCAAGC ACTGGCATGA AGTCATCTTC AGGGTTAGGA
    TCTTCAGGCT CATTGTCTCA GAAAACTCCC CCATCATCTA ATTCTTGTAC AGCATCTTCC
    TCTTCCTTTT CCTCAAGTGG CTCTTCCATG TCATCCTCTC AGAACCAGCA CGGGAGTTCC
    AAAGGGAAAT CCCCCAGCAG AAATAAGAAG CCGTCCTTAA CAGCCGTCAT AGATAAACTG
    AAGCATGGGG TTGTCACCAG TGGCCCTGGA GGTGAAGACC CGATGGATGG CCAGATGGGA
    GTGAGCACAA ATTCTTCTAG CCATCCTATG TCCTCCAAAC ATAACATGTC AGGAGGAGAG
    TTCCAGGGTA AGCGTGAGAA AAGTGATAAA GAAAAATCCA AGGTTTCCAC CTCGGGGGGT
    TCAGTGGATT CGTCTAAGAA GACCTCAGAG TCAAAAAATG TGGGGAGCAC AGGTGTGGCA
    AAAATTATCA TCAGCAAGCA TGATGGGGGT TCCCCCAGCA TTAAAGCCAA AGTGACTTTG
    CAGAAACCTG GGGAAAGTAG TGGAGAAGGG CTCAGACCTC AGATGGCCTC TTCCAAAAAC
    TATGGCTCTC CACTCATCAG TGGTTCCACT CCAAAGCATG AGCGTGGCTC TCCTAGCCAT
    AGTAAGTCAC CAGCATATAC CCCCCAGAAT CTGGACAGTG AAAGTGAGTC AGGCTCCTCC
    ATAGCAGAGA AATCTTATCA GAACAGTCCG AGCTCAGATG ATGGTATCCG ACCTCTTCCA
    GAATATAGCA CAGAAAAGCA TAAGAAGCAC AAAAAAGAAA AGAAGAAAGT AAAAGACAAA
    GATAGAGACC GGGACCGGGA CAAAGACCGG GACAAGAAAA AATCTCATAG CATCAAGCCA
    GAGAGTTGGT CTAAATCACC CATCTCTTCA GACCAGTCCT TGTCTATGAC AAGCAACACA
    ATTTTATCTA CAGACAGGCC CTCAAGGCTC AGCCCTGACT TTATGATTGG GGAGGAAGAT
    GATGATCTTA TGGATGTGGC CCTGATTGGC AATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013972078.1
    CDS188..4423
    Translation

    Target ORF information:

    RefSeq Version XM_014116603.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris mediator complex subunit 1 (MED1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014116603.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTACTTGG CTCTCCAATC CTTAGAACAG GATCTATCTA AAATGGCAGT TATGTATTGG 
    AAAGCAACCA ATGCTGGTCC CTTGGATAAG ATTCTTCATG GAAGTGTTGG CTATCTTACT
    CCAAGAAGTG GGGGTCATTT AATGAACTTG AAGTACTATG CCTCTCCTTC TGACCTGCTG
    GATGATAAGA CTACATCTCC CATCATTTTG CATGAGAATA ATGTTCCTCG ATCATTGGGC
    ATGAACGCAT CAGTGACAAT TGAAGGAACA TCTGCTATGT ATAAACTCCC AATTGCACCA
    TTAATTATGG GGTCACATCC AGTTGACAAC AAATGGACCC CCTCCTTCTC CTCAATCACC
    AGTGCCAACA GTGTTGATCT TCCTGCCTGT TTCTTCTTGA AATTTCCCCA ACCAATCCCA
    GTATCTAGAG CATTTGTTCA GAAACTTCAG AACTGCACAG GAATTCCATT GTTTGAAACT
    CAACCAACTT ATGTACCCCT CTATGAACTG ATCACTCAGT TTGAGCTGTC AAAGGACCCT
    GACCCCATAC CTTTGAATCA CAACATGAGA TTTTATGCTG CTCTTCCAGG TCAGCAGCAC
    TGCTATTTCC TCAACAAGGA TGCTCCTCTT CCAGATGGCC GAAGTCTACA GGGAACCCTA
    GTTAGCAAAA TCACCTTTCA GCACCCTGGC CGAGTTCCTC TTATCCTAAA TCTGATCAGA
    CATCAAGTGG CCTATAACAC CCTAATAGGA AGTTGTGTCA AAAGAACTAT TCTGAAAGAA
    GATTCTCCTG GGCTCCTCCA ATTTGAAGTG TGTCCTCTCT CAGAGTCCCG TTTTAGCGTA
    TCTTTTCAGC ACCCTGTGAA TGACTCCCTG GTGTGTGTGG TAATGGATGT GCAGGACTCA
    ACACATGTGA GCTGTAAACT GTACAAGGGG CTGTCAGATG CACTCATCTG CACAGATGAC
    TTCATTGCCA AAGTTGTTCA AAGATGTATG TCCATCCCTG TGACGATGAG GGCTATTCGG
    AGGAAAGCTG AAACCATTCA GGCCGACACC CCAGCACTGT CCCTCATTGC AGAGACAGTT
    GAAGACATGG TGAAAAAGAA CCTGCCCCCG GCTAGCAGCC CAGGGTATGG CATGACCACA
    GGCAACAACC CAATGAGTGG TACCACTACA CCAACCAACA CCTTTCCGGG GGGTCCCATT
    ACCACCTTGT TTAATATGAG CATGAGCATC AAAGATCGGC ATGAGTCGGT GGGCCATGGG
    GAGGACTTCA GCAAGGTGTC TCAGAACCCA ATTCTTACCA GTTTGTTGCA AATCACAGGG
    AACGGGGGGT CTACCATTGG CTCGAGTCCG ACCCCTCCTC ATCACACGCC GCCACCTGTC
    TCTTCGATGG CCGGCAACAC CAAGAACCAC CCGATGCTCA TGAACCTTCT TAAAGATAAC
    CCTGCCCAGG ATTTCTCAAC CCTTTATGGA AGCAGCCCTT TAGAAAGGCA GAACTCTTCT
    TCCGGCTCAC CCCGGATGGA AATATGCCCG GGAAGCAACA AGACAAAGAA GAAGAAGTCA
    TCAAGATTAC CACCTGACAA ACCCAAGCAC CAGACTGAAG ATGACTTTCA GAGGGAGCTA
    TTTTCAATGG ATGTTGACTC ACAGAACCCT ATCTTTGATG TCAACATGAC AGCAGACACG
    CTGGATACGC CACACATCAC TCCAGCTCCA AGCCAGTGTA GTACTCCCCC AACAACTTAC
    CCGCAACCAG TACCTCACCC CCAACCCAGT ATTCAAAGGA TGGTCCGACT ATCCAGTTCA
    GACAGCATTG GCCCAGATGT AACTGATATC CTTTCAGACA TTGCAGAAGA AGCCTCTAAG
    CTTCCCAGCA CTAATGACGA TTGTCCACCC ATTGGCACCC CTGTTCGAGA TTCTTCAAGC
    TCTGGGCATT CTCAGAGTGC TCTCTTTGAC CCTGATGTCT TTCAAACCAA TAATAATGAA
    AATCCATACA CAGATCCAGC TGACCTAATT GCAGATGCTG CTGGTAGCCC TAGTAGTGAC
    TCTCCTACAA ATCATTTTTT TCCTGATGGA GTAGATTTTA ATCCTGATTT GTTGAACAGC
    CAGAGCCAAA GTGGTTTTGG AGAAGAATAT TTTGATGAAA GCAGCCAAAG CGGAGATAAT
    GATGATTTCA AAGGATTTGC ATCTCAGGCA CTAAATACTT TGGGGGTGCC AATGCTTGGA
    GGTGATAATG GGGAGACTAA GTTTAAAGGC AATAGCCAAG CTGACACAGT TGATTTCAGT
    ATTATAGCAG TGGCTGGTAA GGCTTTAGGT CCTGCAGATC TTATGGAGCA TCACAGTGGT
    AGCCAGAGTC CTTTATTGAC TACTGGGGAC TTAGGGAAAG AAAAGACTCA AAAACGAGTA
    AAGGAAGGCA ATGGCACCAG TAACAGTAGT CTGTCAGGGC CAGGATTAGA TAGCAAACCA
    GGGAAGCGTA GTCGGACCCC TTCTAATGAT GGCAAAAGCA AAGATAAGCC TCCAAAGCGG
    AAGAAGGCAG ACACTGAAGG AAAGTCTCCA TCTCACAGTT CTTCTAACCG ACCTTTCACT
    CCACCTACCA GTACGGGTGG ATCCAAATCA CCAGGCAGTT CAGGAAGATC ACAGACTCCC
    CCAGGTGTTG CCACACCACC TATTCCCAAA ATCACTATTC AGATTCCTAA AGGTACCGTG
    ATGGTGGGCA AGCCCTCATC CCACAGTCAG TATACCAGCA GTGGTTCTGT GTCTTCCTCA
    GGCAGTAAAA GCCACCATAG CCATTCTTCC TCCTCTTCTT CCTCCTCTTC TGCTTCCAAC
    TCAGGCAAGA TGAAAAGCAG TAAATCAGAA GGTTCATCAA GTTCCAAGTT AAGTAGCAGT
    GTATATTCTA GCCAAGGGTC TTCTGGATCT AGTCAGTCCA AAAATTCATC CCAGTCTGGG
    GGGAAGCCAG GCTCCTCTCC CATTACCAAG CATGGACTGA GCAGTGGCTC CGGCAGCACC
    AAGATGAAAC CTCAAGGGAA GCCATCATCA CTTATGAATC CTTCTTTAAG TAAACCAAAC
    ATATCCCCTT CTCATTCAAG GCCACCTGGA GGCTCTGATA AGCTTGCCTC TCCAATGAAG
    CCTGTTCCTG GGACTCCCCC ATCTTCTAAA GCCAAGTCCC CCATCAGTTC AGGTTCTGGT
    GGTTCTCATA TGTCTGGAAG TAGTTCAAGC ACTGGCATGA AGTCATCTTC AGGGTTAGGA
    TCTTCAGGCT CATTGTCTCA GAAAACTCCC CCATCATCTA ATTCTTGTAC AGCATCTTCC
    TCTTCCTTTT CCTCAAGTGG CTCTTCCATG TCATCCTCTC AGAACCAGCA CGGGAGTTCC
    AAAGGGAAAT CCCCCAGCAG AAATAAGAAG CCGTCCTTAA CAGCCGTCAT AGATAAACTG
    AAGCATGGGG TTGTCACCAG TGGCCCTGGA GGTGAAGACC CGATGGATGG CCAGATGGGA
    GTGAGCACAA ATTCTTCTAG CCATCCTATG TCCTCCAAAC ATAACATGTC AGGAGGAGAG
    TTCCAGGGTA AGCGTGAGAA AAGTGATAAA GAAAAATCCA AGGTTTCCAC CTCGGGGGGT
    TCAGTGGATT CGTCTAAGAA GACCTCAGAG TCAAAAAATG TGGGGAGCAC AGGTGTGGCA
    AAAATTATCA TCAGCAAGCA TGATGGGGGT TCCCCCAGCA TTAAAGCCAA AGTGACTTTG
    CAGAAACCTG GGGAAAGTAG TGGAGAAGGG CTCAGACCTC AGATGGCCTC TTCCAAAAAC
    TATGGCTCTC CACTCATCAG TGGTTCCACT CCAAAGCATG AGCGTGGCTC TCCTAGCCAT
    AGTAAGTCAC CAGCATATAC CCCCCAGAAT CTGGACAGTG AAAGTGAGTC AGGCTCCTCC
    ATAGCAGAGA AATCTTATCA GAACAGTCCG AGCTCAGATG ATGGTATCCG ACCTCTTCCA
    GAATATAGCA CAGAAAAGCA TAAGAAGCAC AAAAAAGAAA AGAAGAAAGT AAAAGACAAA
    GATAGAGACC GGGACCGGGA CAAAGACCGG GACAAGAAAA AATCTCATAG CATCAAGCCA
    GAGAGTTGGT CTAAATCACC CATCTCTTCA GACCAGTCCT TGTCTATGAC AAGCAACACA
    ATTTTATCTA CAGACAGGCC CTCAAGGCTC AGCCCTGACT TTATGATTGG GGAGGAAGAT
    GATGATCTTA TGGATGTGGC CCTGATTGGC AATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe11780
    Clone ID Related Accession (Same CDS sequence) XM_858916.4 , XM_858916.5
    Accession Version XM_858916.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4752bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product mediator of RNA polymerase II transcription subunit 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_858916.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAAGGCTC AGGGGGAAAC CGAGGAATCG GAGAAGCTGA GTAAGATGAG TTCTCTCTTG 
    GAACGGCTCC ATGCAAAATT TAACCAAAAT AGACCTTGGA GTGAAACCAT TAAGCTTGTG
    CGTCAAGTCA TGGAGAAGAG AGTTGTGATG AGTTCTGGAG GGCACCAACA TTTGGTCAGC
    TGTTTGGAGA CTTTGCAGAA AGCTCTCAAA GTAACATCTT TACCAGCAAT GACTGACCGC
    TTGGAGTCTA TAGCAAGACA GAATGGACTG GGCTCTCATC TCAGTGCCAG TGGCACTGAA
    TGTTACATCA CGTCAGATAT GTTCTATGTG GAAGTGCAGT TAGATCCTGC AGGACAGCTT
    TGTGATGTAA AAGTGGCTCA CCATGGGGAG AATCCTGTGA GCTGTCCAGA ACTTGTACAG
    CAGCTAAGGG AAAAAAATTT TGATGAATTT TCTAAGCACC TTAAGGGTCT TGTTAATCTT
    TATAACCTTC CAGGGGACAA CAAACTGAAG ACTAAAATGT ACTTGGCTCT CCAATCCTTA
    GAACAGGATC TATCTAAAAT GGCAGTTATG TATTGGAAAG CAACCAATGC TGGTCCCTTG
    GATAAGATTC TTCATGGAAG TGTTGGCTAT CTTACTCCAA GAAGTGGGGG TCATTTAATG
    AACTTGAAGT ACTATGCCTC TCCTTCTGAC CTGCTGGATG ATAAGACTAC ATCTCCCATC
    ATTTTGCATG AGAATAATGT TCCTCGATCA TTGGGCATGA ACGCATCAGT GACAATTGAA
    GGAACATCTG CTATGTATAA ACTCCCAATT GCACCATTAA TTATGGGGTC ACATCCAGTT
    GACAACAAAT GGACCCCCTC CTTCTCCTCA ATCACCAGTG CCAACAGTGT TGATCTTCCT
    GCCTGTTTCT TCTTGAAATT TCCCCAACCA ATCCCAGTAT CTAGAGCATT TGTTCAGAAA
    CTTCAGAACT GCACAGGAAT TCCATTGTTT GAAACTCAAC CAACTTATGT ACCCCTCTAT
    GAACTGATCA CTCAGTTTGA GCTGTCAAAG GACCCTGACC CCATACCTTT GAATCACAAC
    ATGAGATTTT ATGCTGCTCT TCCAGGTCAG CAGCACTGCT ATTTCCTCAA CAAGGATGCT
    CCTCTTCCAG ATGGCCGAAG TCTACAGGGA ACCCTAGTTA GCAAAATCAC CTTTCAGCAC
    CCTGGCCGAG TTCCTCTTAT CCTAAATCTG ATCAGACATC AAGTGGCCTA TAACACCCTA
    ATAGGAAGTT GTGTCAAAAG AACTATTCTG AAAGAAGATT CTCCTGGGCT CCTCCAATTT
    GAAGTGTGTC CTCTCTCAGA GTCCCGTTTT AGCGTATCTT TTCAGCACCC TGTGAATGAC
    TCCCTGGTGT GTGTGGTAAT GGATGTGCAG GACTCAACAC ATGTGAGCTG TAAACTGTAC
    AAGGGGCTGT CAGATGCACT CATCTGCACA GATGACTTCA TTGCCAAAGT TGTTCAAAGA
    TGTATGTCCA TCCCTGTGAC GATGAGGGCT ATTCGGAGGA AAGCTGAAAC CATTCAGGCC
    GACACCCCAG CACTGTCCCT CATTGCAGAG ACAGTTGAAG ACATGGTGAA AAAGAACCTG
    CCCCCGGCTA GCAGCCCAGG GTATGGCATG ACCACAGGCA ACAACCCAAT GAGTGGTACC
    ACTACACCAA CCAACACCTT TCCGGGGGGT CCCATTACCA CCTTGTTTAA TATGAGCATG
    AGCATCAAAG ATCGGCATGA GTCGGTGGGC CATGGGGAGG ACTTCAGCAA GGTGTCTCAG
    AACCCAATTC TTACCAGTTT GTTGCAAATC ACAGGGAACG GGGGGTCTAC CATTGGCTCG
    AGTCCGACCC CTCCTCATCA CACGCCGCCA CCTGTCTCTT CGATGGCCGG CAACACCAAG
    AACCACCCGA TGCTCATGAA CCTTCTTAAA GATAACCCTG CCCAGGATTT CTCAACCCTT
    TATGGAAGCA GCCCTTTAGA AAGGCAGAAC TCTTCTTCCG GCTCACCCCG GATGGAAATA
    TGCCCGGGAA GCAACAAGAC AAAGAAGAAG AAGTCATCAA GATTACCACC TGACAAACCC
    AAGCACCAGA CTGAAGATGA CTTTCAGAGG GAGCTATTTT CAATGGATGT TGACTCACAG
    AACCCTATCT TTGATGTCAA CATGACAGCA GACACGCTGG ATACGCCACA CATCACTCCA
    GCTCCAAGCC AGTGTAGTAC TCCCCCAACA ACTTACCCGC AACCAGTACC TCACCCCCAA
    CCCAGTATTC AAAGGATGGT CCGACTATCC AGTTCAGACA GCATTGGCCC AGATGTAACT
    GATATCCTTT CAGACATTGC AGAAGAAGCC TCTAAGCTTC CCAGCACTAA TGACGATTGT
    CCACCCATTG GCACCCCTGT TCGAGATTCT TCAAGCTCTG GGCATTCTCA GAGTGCTCTC
    TTTGACCCTG ATGTCTTTCA AACCAATAAT AATGAAAATC CATACACAGA TCCAGCTGAC
    CTAATTGCAG ATGCTGCTGG TAGCCCTAGT AGTGACTCTC CTACAAATCA TTTTTTTCCT
    GATGGAGTAG ATTTTAATCC TGATTTGTTG AACAGCCAGA GCCAAAGTGG TTTTGGAGAA
    GAATATTTTG ATGAAAGCAG CCAAAGCGGA GATAATGATG ATTTCAAAGG ATTTGCATCT
    CAGGCACTAA ATACTTTGGG GGTGCCAATG CTTGGAGGTG ATAATGGGGA GACTAAGTTT
    AAAGGCAATA GCCAAGCTGA CACAGTTGAT TTCAGTATTA TAGCAGTGGC TGGTAAGGCT
    TTAGGTCCTG CAGATCTTAT GGAGCATCAC AGTGGTAGCC AGAGTCCTTT ATTGACTACT
    GGGGACTTAG GGAAAGAAAA GACTCAAAAA CGAGTAAAGG AAGGCAATGG CACCAGTAAC
    AGTAGTCTGT CAGGGCCAGG ATTAGATAGC AAACCAGGGA AGCGTAGTCG GACCCCTTCT
    AATGATGGCA AAAGCAAAGA TAAGCCTCCA AAGCGGAAGA AGGCAGACAC TGAAGGAAAG
    TCTCCATCTC ACAGTTCTTC TAACCGACCT TTCACTCCAC CTACCAGTAC GGGTGGATCC
    AAATCACCAG GCAGTTCAGG AAGATCACAG ACTCCCCCAG GTGTTGCCAC ACCACCTATT
    CCCAAAATCA CTATTCAGAT TCCTAAAGGT ACCGTGATGG TGGGCAAGCC CTCATCCCAC
    AGTCAGTATA CCAGCAGTGG TTCTGTGTCT TCCTCAGGCA GTAAAAGCCA CCATAGCCAT
    TCTTCCTCCT CTTCTTCCTC CTCTTCTGCT TCCAACTCAG GCAAGATGAA AAGCAGTAAA
    TCAGAAGGTT CATCAAGTTC CAAGTTAAGT AGCAGTGTAT ATTCTAGCCA AGGGTCTTCT
    GGATCTAGTC AGTCCAAAAA TTCATCCCAG TCTGGGGGGA AGCCAGGCTC CTCTCCCATT
    ACCAAGCATG GACTGAGCAG TGGCTCCGGC AGCACCAAGA TGAAACCTCA AGGGAAGCCA
    TCATCACTTA TGAATCCTTC TTTAAGTAAA CCAAACATAT CCCCTTCTCA TTCAAGGCCA
    CCTGGAGGCT CTGATAAGCT TGCCTCTCCA ATGAAGCCTG TTCCTGGGAC TCCCCCATCT
    TCTAAAGCCA AGTCCCCCAT CAGTTCAGGT TCTGGTGGTT CTCATATGTC TGGAAGTAGT
    TCAAGCACTG GCATGAAGTC ATCTTCAGGG TTAGGATCTT CAGGCTCATT GTCTCAGAAA
    ACTCCCCCAT CATCTAATTC TTGTACAGCA TCTTCCTCTT CCTTTTCCTC AAGTGGCTCT
    TCCATGTCAT CCTCTCAGAA CCAGCACGGG AGTTCCAAAG GGAAATCCCC CAGCAGAAAT
    AAGAAGCCGT CCTTAACAGC CGTCATAGAT AAACTGAAGC ATGGGGTTGT CACCAGTGGC
    CCTGGAGGTG AAGACCCGAT GGATGGCCAG ATGGGAGTGA GCACAAATTC TTCTAGCCAT
    CCTATGTCCT CCAAACATAA CATGTCAGGA GGAGAGTTCC AGGGTAAGCG TGAGAAAAGT
    GATAAAGAAA AATCCAAGGT TTCCACCTCG GGGGGTTCAG TGGATTCGTC TAAGAAGACC
    TCAGAGTCAA AAAATGTGGG GAGCACAGGT GTGGCAAAAA TTATCATCAG CAAGCATGAT
    GGGGGTTCCC CCAGCATTAA AGCCAAAGTG ACTTTGCAGA AACCTGGGGA AAGTAGTGGA
    GAAGGGCTCA GACCTCAGAT GGCCTCTTCC AAAAACTATG GCTCTCCACT CATCAGTGGT
    TCCACTCCAA AGCATGAGCG TGGCTCTCCT AGCCATAGTA AGTCACCAGC ATATACCCCC
    CAGAATCTGG ACAGTGAAAG TGAGTCAGGC TCCTCCATAG CAGAGAAATC TTATCAGAAC
    AGTCCGAGCT CAGATGATGG TATCCGACCT CTTCCAGAAT ATAGCACAGA AAAGCATAAG
    AAGCACAAAA AAGAAAAGAA GAAAGTAAAA GACAAAGATA GAGACCGGGA CCGGGACAAA
    GACCGGGACA AGAAAAAATC TCATAGCATC AAGCCAGAGA GTTGGTCTAA ATCACCCATC
    TCTTCAGACC AGTCCTTGTC TATGACAAGC AACACAATTT TATCTACAGA CAGGCCCTCA
    AGGCTCAGCC CTGACTTTAT GATTGGGGAG GAAGATGATG ATCTTATGGA TGTGGCCCTG
    ATTGGCAATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_864009.2
    CDS215..4966
    Translation

    Target ORF information:

    RefSeq Version XM_858916.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris mediator complex subunit 1 (MED1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_858916.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAAGGCTC AGGGGGAAAC CGAGGAATCG GAGAAGCTGA GTAAGATGAG TTCTCTCTTG 
    GAACGGCTCC ATGCAAAATT TAACCAAAAT AGACCTTGGA GTGAAACCAT TAAGCTTGTG
    CGTCAAGTCA TGGAGAAGAG AGTTGTGATG AGTTCTGGAG GGCACCAACA TTTGGTCAGC
    TGTTTGGAGA CTTTGCAGAA AGCTCTCAAA GTAACATCTT TACCAGCAAT GACTGACCGC
    TTGGAGTCTA TAGCAAGACA GAATGGACTG GGCTCTCATC TCAGTGCCAG TGGCACTGAA
    TGTTACATCA CGTCAGATAT GTTCTATGTG GAAGTGCAGT TAGATCCTGC AGGACAGCTT
    TGTGATGTAA AAGTGGCTCA CCATGGGGAG AATCCTGTGA GCTGTCCAGA ACTTGTACAG
    CAGCTAAGGG AAAAAAATTT TGATGAATTT TCTAAGCACC TTAAGGGTCT TGTTAATCTT
    TATAACCTTC CAGGGGACAA CAAACTGAAG ACTAAAATGT ACTTGGCTCT CCAATCCTTA
    GAACAGGATC TATCTAAAAT GGCAGTTATG TATTGGAAAG CAACCAATGC TGGTCCCTTG
    GATAAGATTC TTCATGGAAG TGTTGGCTAT CTTACTCCAA GAAGTGGGGG TCATTTAATG
    AACTTGAAGT ACTATGCCTC TCCTTCTGAC CTGCTGGATG ATAAGACTAC ATCTCCCATC
    ATTTTGCATG AGAATAATGT TCCTCGATCA TTGGGCATGA ACGCATCAGT GACAATTGAA
    GGAACATCTG CTATGTATAA ACTCCCAATT GCACCATTAA TTATGGGGTC ACATCCAGTT
    GACAACAAAT GGACCCCCTC CTTCTCCTCA ATCACCAGTG CCAACAGTGT TGATCTTCCT
    GCCTGTTTCT TCTTGAAATT TCCCCAACCA ATCCCAGTAT CTAGAGCATT TGTTCAGAAA
    CTTCAGAACT GCACAGGAAT TCCATTGTTT GAAACTCAAC CAACTTATGT ACCCCTCTAT
    GAACTGATCA CTCAGTTTGA GCTGTCAAAG GACCCTGACC CCATACCTTT GAATCACAAC
    ATGAGATTTT ATGCTGCTCT TCCAGGTCAG CAGCACTGCT ATTTCCTCAA CAAGGATGCT
    CCTCTTCCAG ATGGCCGAAG TCTACAGGGA ACCCTAGTTA GCAAAATCAC CTTTCAGCAC
    CCTGGCCGAG TTCCTCTTAT CCTAAATCTG ATCAGACATC AAGTGGCCTA TAACACCCTA
    ATAGGAAGTT GTGTCAAAAG AACTATTCTG AAAGAAGATT CTCCTGGGCT CCTCCAATTT
    GAAGTGTGTC CTCTCTCAGA GTCCCGTTTT AGCGTATCTT TTCAGCACCC TGTGAATGAC
    TCCCTGGTGT GTGTGGTAAT GGATGTGCAG GACTCAACAC ATGTGAGCTG TAAACTGTAC
    AAGGGGCTGT CAGATGCACT CATCTGCACA GATGACTTCA TTGCCAAAGT TGTTCAAAGA
    TGTATGTCCA TCCCTGTGAC GATGAGGGCT ATTCGGAGGA AAGCTGAAAC CATTCAGGCC
    GACACCCCAG CACTGTCCCT CATTGCAGAG ACAGTTGAAG ACATGGTGAA AAAGAACCTG
    CCCCCGGCTA GCAGCCCAGG GTATGGCATG ACCACAGGCA ACAACCCAAT GAGTGGTACC
    ACTACACCAA CCAACACCTT TCCGGGGGGT CCCATTACCA CCTTGTTTAA TATGAGCATG
    AGCATCAAAG ATCGGCATGA GTCGGTGGGC CATGGGGAGG ACTTCAGCAA GGTGTCTCAG
    AACCCAATTC TTACCAGTTT GTTGCAAATC ACAGGGAACG GGGGGTCTAC CATTGGCTCG
    AGTCCGACCC CTCCTCATCA CACGCCGCCA CCTGTCTCTT CGATGGCCGG CAACACCAAG
    AACCACCCGA TGCTCATGAA CCTTCTTAAA GATAACCCTG CCCAGGATTT CTCAACCCTT
    TATGGAAGCA GCCCTTTAGA AAGGCAGAAC TCTTCTTCCG GCTCACCCCG GATGGAAATA
    TGCCCGGGAA GCAACAAGAC AAAGAAGAAG AAGTCATCAA GATTACCACC TGACAAACCC
    AAGCACCAGA CTGAAGATGA CTTTCAGAGG GAGCTATTTT CAATGGATGT TGACTCACAG
    AACCCTATCT TTGATGTCAA CATGACAGCA GACACGCTGG ATACGCCACA CATCACTCCA
    GCTCCAAGCC AGTGTAGTAC TCCCCCAACA ACTTACCCGC AACCAGTACC TCACCCCCAA
    CCCAGTATTC AAAGGATGGT CCGACTATCC AGTTCAGACA GCATTGGCCC AGATGTAACT
    GATATCCTTT CAGACATTGC AGAAGAAGCC TCTAAGCTTC CCAGCACTAA TGACGATTGT
    CCACCCATTG GCACCCCTGT TCGAGATTCT TCAAGCTCTG GGCATTCTCA GAGTGCTCTC
    TTTGACCCTG ATGTCTTTCA AACCAATAAT AATGAAAATC CATACACAGA TCCAGCTGAC
    CTAATTGCAG ATGCTGCTGG TAGCCCTAGT AGTGACTCTC CTACAAATCA TTTTTTTCCT
    GATGGAGTAG ATTTTAATCC TGATTTGTTG AACAGCCAGA GCCAAAGTGG TTTTGGAGAA
    GAATATTTTG ATGAAAGCAG CCAAAGCGGA GATAATGATG ATTTCAAAGG ATTTGCATCT
    CAGGCACTAA ATACTTTGGG GGTGCCAATG CTTGGAGGTG ATAATGGGGA GACTAAGTTT
    AAAGGCAATA GCCAAGCTGA CACAGTTGAT TTCAGTATTA TAGCAGTGGC TGGTAAGGCT
    TTAGGTCCTG CAGATCTTAT GGAGCATCAC AGTGGTAGCC AGAGTCCTTT ATTGACTACT
    GGGGACTTAG GGAAAGAAAA GACTCAAAAA CGAGTAAAGG AAGGCAATGG CACCAGTAAC
    AGTAGTCTGT CAGGGCCAGG ATTAGATAGC AAACCAGGGA AGCGTAGTCG GACCCCTTCT
    AATGATGGCA AAAGCAAAGA TAAGCCTCCA AAGCGGAAGA AGGCAGACAC TGAAGGAAAG
    TCTCCATCTC ACAGTTCTTC TAACCGACCT TTCACTCCAC CTACCAGTAC GGGTGGATCC
    AAATCACCAG GCAGTTCAGG AAGATCACAG ACTCCCCCAG GTGTTGCCAC ACCACCTATT
    CCCAAAATCA CTATTCAGAT TCCTAAAGGT ACCGTGATGG TGGGCAAGCC CTCATCCCAC
    AGTCAGTATA CCAGCAGTGG TTCTGTGTCT TCCTCAGGCA GTAAAAGCCA CCATAGCCAT
    TCTTCCTCCT CTTCTTCCTC CTCTTCTGCT TCCAACTCAG GCAAGATGAA AAGCAGTAAA
    TCAGAAGGTT CATCAAGTTC CAAGTTAAGT AGCAGTGTAT ATTCTAGCCA AGGGTCTTCT
    GGATCTAGTC AGTCCAAAAA TTCATCCCAG TCTGGGGGGA AGCCAGGCTC CTCTCCCATT
    ACCAAGCATG GACTGAGCAG TGGCTCCGGC AGCACCAAGA TGAAACCTCA AGGGAAGCCA
    TCATCACTTA TGAATCCTTC TTTAAGTAAA CCAAACATAT CCCCTTCTCA TTCAAGGCCA
    CCTGGAGGCT CTGATAAGCT TGCCTCTCCA ATGAAGCCTG TTCCTGGGAC TCCCCCATCT
    TCTAAAGCCA AGTCCCCCAT CAGTTCAGGT TCTGGTGGTT CTCATATGTC TGGAAGTAGT
    TCAAGCACTG GCATGAAGTC ATCTTCAGGG TTAGGATCTT CAGGCTCATT GTCTCAGAAA
    ACTCCCCCAT CATCTAATTC TTGTACAGCA TCTTCCTCTT CCTTTTCCTC AAGTGGCTCT
    TCCATGTCAT CCTCTCAGAA CCAGCACGGG AGTTCCAAAG GGAAATCCCC CAGCAGAAAT
    AAGAAGCCGT CCTTAACAGC CGTCATAGAT AAACTGAAGC ATGGGGTTGT CACCAGTGGC
    CCTGGAGGTG AAGACCCGAT GGATGGCCAG ATGGGAGTGA GCACAAATTC TTCTAGCCAT
    CCTATGTCCT CCAAACATAA CATGTCAGGA GGAGAGTTCC AGGGTAAGCG TGAGAAAAGT
    GATAAAGAAA AATCCAAGGT TTCCACCTCG GGGGGTTCAG TGGATTCGTC TAAGAAGACC
    TCAGAGTCAA AAAATGTGGG GAGCACAGGT GTGGCAAAAA TTATCATCAG CAAGCATGAT
    GGGGGTTCCC CCAGCATTAA AGCCAAAGTG ACTTTGCAGA AACCTGGGGA AAGTAGTGGA
    GAAGGGCTCA GACCTCAGAT GGCCTCTTCC AAAAACTATG GCTCTCCACT CATCAGTGGT
    TCCACTCCAA AGCATGAGCG TGGCTCTCCT AGCCATAGTA AGTCACCAGC ATATACCCCC
    CAGAATCTGG ACAGTGAAAG TGAGTCAGGC TCCTCCATAG CAGAGAAATC TTATCAGAAC
    AGTCCGAGCT CAGATGATGG TATCCGACCT CTTCCAGAAT ATAGCACAGA AAAGCATAAG
    AAGCACAAAA AAGAAAAGAA GAAAGTAAAA GACAAAGATA GAGACCGGGA CCGGGACAAA
    GACCGGGACA AGAAAAAATC TCATAGCATC AAGCCAGAGA GTTGGTCTAA ATCACCCATC
    TCTTCAGACC AGTCCTTGTC TATGACAAGC AACACAATTT TATCTACAGA CAGGCCCTCA
    AGGCTCAGCC CTGACTTTAT GATTGGGGAG GAAGATGATG ATCTTATGGA TGTGGCCCTG
    ATTGGCAATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe11780
    Clone ID Related Accession (Same CDS sequence) XM_858916.4 , XM_858916.5
    Accession Version XM_858916.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4752bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product mediator of RNA polymerase II transcription subunit 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_858916.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAAGGCTC AGGGGGAAAC CGAGGAATCG GAGAAGCTGA GTAAGATGAG TTCTCTCTTG 
    GAACGGCTCC ATGCAAAATT TAACCAAAAT AGACCTTGGA GTGAAACCAT TAAGCTTGTG
    CGTCAAGTCA TGGAGAAGAG AGTTGTGATG AGTTCTGGAG GGCACCAACA TTTGGTCAGC
    TGTTTGGAGA CTTTGCAGAA AGCTCTCAAA GTAACATCTT TACCAGCAAT GACTGACCGC
    TTGGAGTCTA TAGCAAGACA GAATGGACTG GGCTCTCATC TCAGTGCCAG TGGCACTGAA
    TGTTACATCA CGTCAGATAT GTTCTATGTG GAAGTGCAGT TAGATCCTGC AGGACAGCTT
    TGTGATGTAA AAGTGGCTCA CCATGGGGAG AATCCTGTGA GCTGTCCAGA ACTTGTACAG
    CAGCTAAGGG AAAAAAATTT TGATGAATTT TCTAAGCACC TTAAGGGTCT TGTTAATCTT
    TATAACCTTC CAGGGGACAA CAAACTGAAG ACTAAAATGT ACTTGGCTCT CCAATCCTTA
    GAACAGGATC TATCTAAAAT GGCAGTTATG TATTGGAAAG CAACCAATGC TGGTCCCTTG
    GATAAGATTC TTCATGGAAG TGTTGGCTAT CTTACTCCAA GAAGTGGGGG TCATTTAATG
    AACTTGAAGT ACTATGCCTC TCCTTCTGAC CTGCTGGATG ATAAGACTAC ATCTCCCATC
    ATTTTGCATG AGAATAATGT TCCTCGATCA TTGGGCATGA ACGCATCAGT GACAATTGAA
    GGAACATCTG CTATGTATAA ACTCCCAATT GCACCATTAA TTATGGGGTC ACATCCAGTT
    GACAACAAAT GGACCCCCTC CTTCTCCTCA ATCACCAGTG CCAACAGTGT TGATCTTCCT
    GCCTGTTTCT TCTTGAAATT TCCCCAACCA ATCCCAGTAT CTAGAGCATT TGTTCAGAAA
    CTTCAGAACT GCACAGGAAT TCCATTGTTT GAAACTCAAC CAACTTATGT ACCCCTCTAT
    GAACTGATCA CTCAGTTTGA GCTGTCAAAG GACCCTGACC CCATACCTTT GAATCACAAC
    ATGAGATTTT ATGCTGCTCT TCCAGGTCAG CAGCACTGCT ATTTCCTCAA CAAGGATGCT
    CCTCTTCCAG ATGGCCGAAG TCTACAGGGA ACCCTAGTTA GCAAAATCAC CTTTCAGCAC
    CCTGGCCGAG TTCCTCTTAT CCTAAATCTG ATCAGACATC AAGTGGCCTA TAACACCCTA
    ATAGGAAGTT GTGTCAAAAG AACTATTCTG AAAGAAGATT CTCCTGGGCT CCTCCAATTT
    GAAGTGTGTC CTCTCTCAGA GTCCCGTTTT AGCGTATCTT TTCAGCACCC TGTGAATGAC
    TCCCTGGTGT GTGTGGTAAT GGATGTGCAG GACTCAACAC ATGTGAGCTG TAAACTGTAC
    AAGGGGCTGT CAGATGCACT CATCTGCACA GATGACTTCA TTGCCAAAGT TGTTCAAAGA
    TGTATGTCCA TCCCTGTGAC GATGAGGGCT ATTCGGAGGA AAGCTGAAAC CATTCAGGCC
    GACACCCCAG CACTGTCCCT CATTGCAGAG ACAGTTGAAG ACATGGTGAA AAAGAACCTG
    CCCCCGGCTA GCAGCCCAGG GTATGGCATG ACCACAGGCA ACAACCCAAT GAGTGGTACC
    ACTACACCAA CCAACACCTT TCCGGGGGGT CCCATTACCA CCTTGTTTAA TATGAGCATG
    AGCATCAAAG ATCGGCATGA GTCGGTGGGC CATGGGGAGG ACTTCAGCAA GGTGTCTCAG
    AACCCAATTC TTACCAGTTT GTTGCAAATC ACAGGGAACG GGGGGTCTAC CATTGGCTCG
    AGTCCGACCC CTCCTCATCA CACGCCGCCA CCTGTCTCTT CGATGGCCGG CAACACCAAG
    AACCACCCGA TGCTCATGAA CCTTCTTAAA GATAACCCTG CCCAGGATTT CTCAACCCTT
    TATGGAAGCA GCCCTTTAGA AAGGCAGAAC TCTTCTTCCG GCTCACCCCG GATGGAAATA
    TGCCCGGGAA GCAACAAGAC AAAGAAGAAG AAGTCATCAA GATTACCACC TGACAAACCC
    AAGCACCAGA CTGAAGATGA CTTTCAGAGG GAGCTATTTT CAATGGATGT TGACTCACAG
    AACCCTATCT TTGATGTCAA CATGACAGCA GACACGCTGG ATACGCCACA CATCACTCCA
    GCTCCAAGCC AGTGTAGTAC TCCCCCAACA ACTTACCCGC AACCAGTACC TCACCCCCAA
    CCCAGTATTC AAAGGATGGT CCGACTATCC AGTTCAGACA GCATTGGCCC AGATGTAACT
    GATATCCTTT CAGACATTGC AGAAGAAGCC TCTAAGCTTC CCAGCACTAA TGACGATTGT
    CCACCCATTG GCACCCCTGT TCGAGATTCT TCAAGCTCTG GGCATTCTCA GAGTGCTCTC
    TTTGACCCTG ATGTCTTTCA AACCAATAAT AATGAAAATC CATACACAGA TCCAGCTGAC
    CTAATTGCAG ATGCTGCTGG TAGCCCTAGT AGTGACTCTC CTACAAATCA TTTTTTTCCT
    GATGGAGTAG ATTTTAATCC TGATTTGTTG AACAGCCAGA GCCAAAGTGG TTTTGGAGAA
    GAATATTTTG ATGAAAGCAG CCAAAGCGGA GATAATGATG ATTTCAAAGG ATTTGCATCT
    CAGGCACTAA ATACTTTGGG GGTGCCAATG CTTGGAGGTG ATAATGGGGA GACTAAGTTT
    AAAGGCAATA GCCAAGCTGA CACAGTTGAT TTCAGTATTA TAGCAGTGGC TGGTAAGGCT
    TTAGGTCCTG CAGATCTTAT GGAGCATCAC AGTGGTAGCC AGAGTCCTTT ATTGACTACT
    GGGGACTTAG GGAAAGAAAA GACTCAAAAA CGAGTAAAGG AAGGCAATGG CACCAGTAAC
    AGTAGTCTGT CAGGGCCAGG ATTAGATAGC AAACCAGGGA AGCGTAGTCG GACCCCTTCT
    AATGATGGCA AAAGCAAAGA TAAGCCTCCA AAGCGGAAGA AGGCAGACAC TGAAGGAAAG
    TCTCCATCTC ACAGTTCTTC TAACCGACCT TTCACTCCAC CTACCAGTAC GGGTGGATCC
    AAATCACCAG GCAGTTCAGG AAGATCACAG ACTCCCCCAG GTGTTGCCAC ACCACCTATT
    CCCAAAATCA CTATTCAGAT TCCTAAAGGT ACCGTGATGG TGGGCAAGCC CTCATCCCAC
    AGTCAGTATA CCAGCAGTGG TTCTGTGTCT TCCTCAGGCA GTAAAAGCCA CCATAGCCAT
    TCTTCCTCCT CTTCTTCCTC CTCTTCTGCT TCCAACTCAG GCAAGATGAA AAGCAGTAAA
    TCAGAAGGTT CATCAAGTTC CAAGTTAAGT AGCAGTGTAT ATTCTAGCCA AGGGTCTTCT
    GGATCTAGTC AGTCCAAAAA TTCATCCCAG TCTGGGGGGA AGCCAGGCTC CTCTCCCATT
    ACCAAGCATG GACTGAGCAG TGGCTCCGGC AGCACCAAGA TGAAACCTCA AGGGAAGCCA
    TCATCACTTA TGAATCCTTC TTTAAGTAAA CCAAACATAT CCCCTTCTCA TTCAAGGCCA
    CCTGGAGGCT CTGATAAGCT TGCCTCTCCA ATGAAGCCTG TTCCTGGGAC TCCCCCATCT
    TCTAAAGCCA AGTCCCCCAT CAGTTCAGGT TCTGGTGGTT CTCATATGTC TGGAAGTAGT
    TCAAGCACTG GCATGAAGTC ATCTTCAGGG TTAGGATCTT CAGGCTCATT GTCTCAGAAA
    ACTCCCCCAT CATCTAATTC TTGTACAGCA TCTTCCTCTT CCTTTTCCTC AAGTGGCTCT
    TCCATGTCAT CCTCTCAGAA CCAGCACGGG AGTTCCAAAG GGAAATCCCC CAGCAGAAAT
    AAGAAGCCGT CCTTAACAGC CGTCATAGAT AAACTGAAGC ATGGGGTTGT CACCAGTGGC
    CCTGGAGGTG AAGACCCGAT GGATGGCCAG ATGGGAGTGA GCACAAATTC TTCTAGCCAT
    CCTATGTCCT CCAAACATAA CATGTCAGGA GGAGAGTTCC AGGGTAAGCG TGAGAAAAGT
    GATAAAGAAA AATCCAAGGT TTCCACCTCG GGGGGTTCAG TGGATTCGTC TAAGAAGACC
    TCAGAGTCAA AAAATGTGGG GAGCACAGGT GTGGCAAAAA TTATCATCAG CAAGCATGAT
    GGGGGTTCCC CCAGCATTAA AGCCAAAGTG ACTTTGCAGA AACCTGGGGA AAGTAGTGGA
    GAAGGGCTCA GACCTCAGAT GGCCTCTTCC AAAAACTATG GCTCTCCACT CATCAGTGGT
    TCCACTCCAA AGCATGAGCG TGGCTCTCCT AGCCATAGTA AGTCACCAGC ATATACCCCC
    CAGAATCTGG ACAGTGAAAG TGAGTCAGGC TCCTCCATAG CAGAGAAATC TTATCAGAAC
    AGTCCGAGCT CAGATGATGG TATCCGACCT CTTCCAGAAT ATAGCACAGA AAAGCATAAG
    AAGCACAAAA AAGAAAAGAA GAAAGTAAAA GACAAAGATA GAGACCGGGA CCGGGACAAA
    GACCGGGACA AGAAAAAATC TCATAGCATC AAGCCAGAGA GTTGGTCTAA ATCACCCATC
    TCTTCAGACC AGTCCTTGTC TATGACAAGC AACACAATTT TATCTACAGA CAGGCCCTCA
    AGGCTCAGCC CTGACTTTAT GATTGGGGAG GAAGATGATG ATCTTATGGA TGTGGCCCTG
    ATTGGCAATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_864009.2
    CDS223..4974
    Translation

    Target ORF information:

    RefSeq Version XM_858916.5
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris mediator complex subunit 1 (MED1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_858916.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAAGGCTC AGGGGGAAAC CGAGGAATCG GAGAAGCTGA GTAAGATGAG TTCTCTCTTG 
    GAACGGCTCC ATGCAAAATT TAACCAAAAT AGACCTTGGA GTGAAACCAT TAAGCTTGTG
    CGTCAAGTCA TGGAGAAGAG AGTTGTGATG AGTTCTGGAG GGCACCAACA TTTGGTCAGC
    TGTTTGGAGA CTTTGCAGAA AGCTCTCAAA GTAACATCTT TACCAGCAAT GACTGACCGC
    TTGGAGTCTA TAGCAAGACA GAATGGACTG GGCTCTCATC TCAGTGCCAG TGGCACTGAA
    TGTTACATCA CGTCAGATAT GTTCTATGTG GAAGTGCAGT TAGATCCTGC AGGACAGCTT
    TGTGATGTAA AAGTGGCTCA CCATGGGGAG AATCCTGTGA GCTGTCCAGA ACTTGTACAG
    CAGCTAAGGG AAAAAAATTT TGATGAATTT TCTAAGCACC TTAAGGGTCT TGTTAATCTT
    TATAACCTTC CAGGGGACAA CAAACTGAAG ACTAAAATGT ACTTGGCTCT CCAATCCTTA
    GAACAGGATC TATCTAAAAT GGCAGTTATG TATTGGAAAG CAACCAATGC TGGTCCCTTG
    GATAAGATTC TTCATGGAAG TGTTGGCTAT CTTACTCCAA GAAGTGGGGG TCATTTAATG
    AACTTGAAGT ACTATGCCTC TCCTTCTGAC CTGCTGGATG ATAAGACTAC ATCTCCCATC
    ATTTTGCATG AGAATAATGT TCCTCGATCA TTGGGCATGA ACGCATCAGT GACAATTGAA
    GGAACATCTG CTATGTATAA ACTCCCAATT GCACCATTAA TTATGGGGTC ACATCCAGTT
    GACAACAAAT GGACCCCCTC CTTCTCCTCA ATCACCAGTG CCAACAGTGT TGATCTTCCT
    GCCTGTTTCT TCTTGAAATT TCCCCAACCA ATCCCAGTAT CTAGAGCATT TGTTCAGAAA
    CTTCAGAACT GCACAGGAAT TCCATTGTTT GAAACTCAAC CAACTTATGT ACCCCTCTAT
    GAACTGATCA CTCAGTTTGA GCTGTCAAAG GACCCTGACC CCATACCTTT GAATCACAAC
    ATGAGATTTT ATGCTGCTCT TCCAGGTCAG CAGCACTGCT ATTTCCTCAA CAAGGATGCT
    CCTCTTCCAG ATGGCCGAAG TCTACAGGGA ACCCTAGTTA GCAAAATCAC CTTTCAGCAC
    CCTGGCCGAG TTCCTCTTAT CCTAAATCTG ATCAGACATC AAGTGGCCTA TAACACCCTA
    ATAGGAAGTT GTGTCAAAAG AACTATTCTG AAAGAAGATT CTCCTGGGCT CCTCCAATTT
    GAAGTGTGTC CTCTCTCAGA GTCCCGTTTT AGCGTATCTT TTCAGCACCC TGTGAATGAC
    TCCCTGGTGT GTGTGGTAAT GGATGTGCAG GACTCAACAC ATGTGAGCTG TAAACTGTAC
    AAGGGGCTGT CAGATGCACT CATCTGCACA GATGACTTCA TTGCCAAAGT TGTTCAAAGA
    TGTATGTCCA TCCCTGTGAC GATGAGGGCT ATTCGGAGGA AAGCTGAAAC CATTCAGGCC
    GACACCCCAG CACTGTCCCT CATTGCAGAG ACAGTTGAAG ACATGGTGAA AAAGAACCTG
    CCCCCGGCTA GCAGCCCAGG GTATGGCATG ACCACAGGCA ACAACCCAAT GAGTGGTACC
    ACTACACCAA CCAACACCTT TCCGGGGGGT CCCATTACCA CCTTGTTTAA TATGAGCATG
    AGCATCAAAG ATCGGCATGA GTCGGTGGGC CATGGGGAGG ACTTCAGCAA GGTGTCTCAG
    AACCCAATTC TTACCAGTTT GTTGCAAATC ACAGGGAACG GGGGGTCTAC CATTGGCTCG
    AGTCCGACCC CTCCTCATCA CACGCCGCCA CCTGTCTCTT CGATGGCCGG CAACACCAAG
    AACCACCCGA TGCTCATGAA CCTTCTTAAA GATAACCCTG CCCAGGATTT CTCAACCCTT
    TATGGAAGCA GCCCTTTAGA AAGGCAGAAC TCTTCTTCCG GCTCACCCCG GATGGAAATA
    TGCCCGGGAA GCAACAAGAC AAAGAAGAAG AAGTCATCAA GATTACCACC TGACAAACCC
    AAGCACCAGA CTGAAGATGA CTTTCAGAGG GAGCTATTTT CAATGGATGT TGACTCACAG
    AACCCTATCT TTGATGTCAA CATGACAGCA GACACGCTGG ATACGCCACA CATCACTCCA
    GCTCCAAGCC AGTGTAGTAC TCCCCCAACA ACTTACCCGC AACCAGTACC TCACCCCCAA
    CCCAGTATTC AAAGGATGGT CCGACTATCC AGTTCAGACA GCATTGGCCC AGATGTAACT
    GATATCCTTT CAGACATTGC AGAAGAAGCC TCTAAGCTTC CCAGCACTAA TGACGATTGT
    CCACCCATTG GCACCCCTGT TCGAGATTCT TCAAGCTCTG GGCATTCTCA GAGTGCTCTC
    TTTGACCCTG ATGTCTTTCA AACCAATAAT AATGAAAATC CATACACAGA TCCAGCTGAC
    CTAATTGCAG ATGCTGCTGG TAGCCCTAGT AGTGACTCTC CTACAAATCA TTTTTTTCCT
    GATGGAGTAG ATTTTAATCC TGATTTGTTG AACAGCCAGA GCCAAAGTGG TTTTGGAGAA
    GAATATTTTG ATGAAAGCAG CCAAAGCGGA GATAATGATG ATTTCAAAGG ATTTGCATCT
    CAGGCACTAA ATACTTTGGG GGTGCCAATG CTTGGAGGTG ATAATGGGGA GACTAAGTTT
    AAAGGCAATA GCCAAGCTGA CACAGTTGAT TTCAGTATTA TAGCAGTGGC TGGTAAGGCT
    TTAGGTCCTG CAGATCTTAT GGAGCATCAC AGTGGTAGCC AGAGTCCTTT ATTGACTACT
    GGGGACTTAG GGAAAGAAAA GACTCAAAAA CGAGTAAAGG AAGGCAATGG CACCAGTAAC
    AGTAGTCTGT CAGGGCCAGG ATTAGATAGC AAACCAGGGA AGCGTAGTCG GACCCCTTCT
    AATGATGGCA AAAGCAAAGA TAAGCCTCCA AAGCGGAAGA AGGCAGACAC TGAAGGAAAG
    TCTCCATCTC ACAGTTCTTC TAACCGACCT TTCACTCCAC CTACCAGTAC GGGTGGATCC
    AAATCACCAG GCAGTTCAGG AAGATCACAG ACTCCCCCAG GTGTTGCCAC ACCACCTATT
    CCCAAAATCA CTATTCAGAT TCCTAAAGGT ACCGTGATGG TGGGCAAGCC CTCATCCCAC
    AGTCAGTATA CCAGCAGTGG TTCTGTGTCT TCCTCAGGCA GTAAAAGCCA CCATAGCCAT
    TCTTCCTCCT CTTCTTCCTC CTCTTCTGCT TCCAACTCAG GCAAGATGAA AAGCAGTAAA
    TCAGAAGGTT CATCAAGTTC CAAGTTAAGT AGCAGTGTAT ATTCTAGCCA AGGGTCTTCT
    GGATCTAGTC AGTCCAAAAA TTCATCCCAG TCTGGGGGGA AGCCAGGCTC CTCTCCCATT
    ACCAAGCATG GACTGAGCAG TGGCTCCGGC AGCACCAAGA TGAAACCTCA AGGGAAGCCA
    TCATCACTTA TGAATCCTTC TTTAAGTAAA CCAAACATAT CCCCTTCTCA TTCAAGGCCA
    CCTGGAGGCT CTGATAAGCT TGCCTCTCCA ATGAAGCCTG TTCCTGGGAC TCCCCCATCT
    TCTAAAGCCA AGTCCCCCAT CAGTTCAGGT TCTGGTGGTT CTCATATGTC TGGAAGTAGT
    TCAAGCACTG GCATGAAGTC ATCTTCAGGG TTAGGATCTT CAGGCTCATT GTCTCAGAAA
    ACTCCCCCAT CATCTAATTC TTGTACAGCA TCTTCCTCTT CCTTTTCCTC AAGTGGCTCT
    TCCATGTCAT CCTCTCAGAA CCAGCACGGG AGTTCCAAAG GGAAATCCCC CAGCAGAAAT
    AAGAAGCCGT CCTTAACAGC CGTCATAGAT AAACTGAAGC ATGGGGTTGT CACCAGTGGC
    CCTGGAGGTG AAGACCCGAT GGATGGCCAG ATGGGAGTGA GCACAAATTC TTCTAGCCAT
    CCTATGTCCT CCAAACATAA CATGTCAGGA GGAGAGTTCC AGGGTAAGCG TGAGAAAAGT
    GATAAAGAAA AATCCAAGGT TTCCACCTCG GGGGGTTCAG TGGATTCGTC TAAGAAGACC
    TCAGAGTCAA AAAATGTGGG GAGCACAGGT GTGGCAAAAA TTATCATCAG CAAGCATGAT
    GGGGGTTCCC CCAGCATTAA AGCCAAAGTG ACTTTGCAGA AACCTGGGGA AAGTAGTGGA
    GAAGGGCTCA GACCTCAGAT GGCCTCTTCC AAAAACTATG GCTCTCCACT CATCAGTGGT
    TCCACTCCAA AGCATGAGCG TGGCTCTCCT AGCCATAGTA AGTCACCAGC ATATACCCCC
    CAGAATCTGG ACAGTGAAAG TGAGTCAGGC TCCTCCATAG CAGAGAAATC TTATCAGAAC
    AGTCCGAGCT CAGATGATGG TATCCGACCT CTTCCAGAAT ATAGCACAGA AAAGCATAAG
    AAGCACAAAA AAGAAAAGAA GAAAGTAAAA GACAAAGATA GAGACCGGGA CCGGGACAAA
    GACCGGGACA AGAAAAAATC TCATAGCATC AAGCCAGAGA GTTGGTCTAA ATCACCCATC
    TCTTCAGACC AGTCCTTGTC TATGACAAGC AACACAATTT TATCTACAGA CAGGCCCTCA
    AGGCTCAGCC CTGACTTTAT GATTGGGGAG GAAGATGATG ATCTTATGGA TGTGGCCCTG
    ATTGGCAATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.