AGRN cDNA ORF clone, Canis lupus familiaris(Dog)

The following AGRN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AGRN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe06700 XM_005620365.3
Latest version!
Canis lupus familiaris agrin (AGRN), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe06701 XM_005620366.3
Latest version!
Canis lupus familiaris agrin (AGRN), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe06693 XM_005620359.3
Latest version!
Canis lupus familiaris agrin (AGRN), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe06694 XM_005620360.3
Latest version!
Canis lupus familiaris agrin (AGRN), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe06695 XM_005620361.3
Latest version!
Canis lupus familiaris agrin (AGRN), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe06696 XM_005620362.3
Latest version!
Canis lupus familiaris agrin (AGRN), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe06697 XM_005620363.2
Latest version!
Canis lupus familiaris agrin (AGRN), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe06698 XM_005620364.2
Latest version!
Canis lupus familiaris agrin (AGRN), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe06699 XM_536713.6
Latest version!
Canis lupus familiaris agrin (AGRN), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
View Old Accession Versions >>
OCe06694 XM_005620360.2 Canis lupus familiaris agrin (AGRN), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe06697 XM_005620363.1 Canis lupus familiaris agrin (AGRN), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe06698 XM_005620364.1 Canis lupus familiaris agrin (AGRN), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe06701 XM_005620366.2 Canis lupus familiaris agrin (AGRN), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe06693 XM_005620359.2 Canis lupus familiaris agrin (AGRN), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe06696 XM_005620362.2 Canis lupus familiaris agrin (AGRN), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe06700 XM_005620365.2 Canis lupus familiaris agrin (AGRN), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe06695 XM_005620361.2 Canis lupus familiaris agrin (AGRN), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe06699 XM_536713.5 Canis lupus familiaris agrin (AGRN), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe06700
    Clone ID Related Accession (Same CDS sequence) XM_005620365.2 , XM_005620365.3
    Accession Version XM_005620365.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5907bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product agrin isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726058.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005620365.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCCTCCGC CACCTTTGGA GCGGGACCCC CGGCAGCAGC GTGGGGTCTC CCTGCTGGTG 
    CGGTATTTTA TGATTCCCTG CAACGTCTGC CTGATTCTGC TGGCCACGTC CACGCTGGGC
    TTCGCGGTGC TGCTTTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC ACCAGCGCCC
    CCAACGCCTC CAGATGCATG CCGAGGAATG CTGTGTGGCT TCGGTGCTGT GTGTGAGCCC
    AGTGCAGGGG GTTCAGGCCG AGCCTCCTGC GTGTGCAAGA AGAGTGCCTG TCCCGCCGTG
    GTGGCGCCCG TGTGTGGCTC TGACGCCTCC ACCTACAGCA ATGAGTGTGA GCTGCAGCGG
    GCGCAGTGCA GCCAGCAGCG CCGCATCCGC CTACTCAGTC GCGGACCCTG TGGTTCCCGG
    GACCCCTGCT CCAATGTGAC ATGCAGCTTC GGCAGCACCT GTGTGCGCTC AGCAGATGGG
    CAGACAGCCA CATGCCTGTG CCCTGCCACC TGCCGGGGGC CCCCGGAGGG CCCCGTGTGT
    GGGAGCGACG GTGCTGACTA CCCCAGTGAG TGCCAGCTCC TGCGCCTCGC GTGCACTCGC
    CAGGAGAACA TCGTCAAGAA GTTTGACGGC CCTTGTGACC CCTGCCAGGG CACCCACCAT
    GACCTGAGCC GTGTCTGCCG CGTTCACCCA CGCACGCGGC ATCCTGAGAT GCTCCTCCGG
    CCAGAGAGTT GCCCTTCTCA GCCAGCACCT GTGTGCGGGG ATGATGGAGT CACCTATGAC
    AACGACTGCA TAATGGGCCG CACGGGAGCC ACCCGTGGCC TTGTCCTCCA GAAAGTGCGC
    TCTGGCCAGT GCCAGCCTCA AGACCAGTGT CCAGATGTGT GCAGGTTCAA TGCTGTGTGT
    CTGTCCCGCC GAGGCCGCCC CCGCTGCTCC TGTGACCGTG TCATCTGTGA TGGGGCTTAT
    AGACCCGTGT GTGCTCACGA CGGGCACACG TATGATAATG ACTGCTGGCG TCAGCAAGCT
    GAGTGTCGGC AGCAGCGCAC CATTCCCGCC AAGCACCAGG GCCCGTGTGA CCAGCCCCTG
    TCCCCGTGCC TTGGGGTGCG GTGTCCTTTT GGGGCAACAT GTGCCGTGAA GAATGGGGAG
    GCTGAGTGCG TGTGCCAGCA GGTGTGTTCG GGCATCTATG ATCCTGTGTG TGGCAGTGAT
    GGTGTCACAT ATGGCAGCAC ATGTGAACTG GAAGCCACGG CCTGCGCCCT TGGACGGGAG
    ATCCGGGTGG CTCGCAGAGG ACCCTGTGAC CGCTGTGGGC AGTGCCGCTT TGGAGCTCTG
    TGTGAGGCTG AGACTGGGCG CTGTGTGTGC CCCTCCGAGT GCGTGGCCTC GGCTCAACCT
    GTGTGCGGTT CTGATGGGCA CACGTATGCC AGCGAGTGTG AGCTGCACGT CCACGCCTGC
    ACACACCAGA TCAGCCTGCA TGTGGTCTCA GCTGGACACT GCCAGACCTG CGGAGACACA
    GTGTGTGCTT TCGGGGCAGT GTGCTTGGCT GGGCAATGTG TGTGTCCCCG ATGTGAGCGA
    CCCCCACCCG GGCCCGTGTG TGGCAGCGAT GGTGTCACCT ACGACAGTGC CTGTCATCTC
    CGAGAAGCTG CCTGCCAGCA ACAGACACAG ATTGAGGAGG CCCGGGCGGG GCCCTGTGAG
    CAGGCTGAGT GTGGCTCTGG AGGCTCTGGC TCCGGAGAGG ATGGCGAGTG TGAGCAGGAG
    CTGTGCCAGC AGCATGGCGG TGTCTGGGAT GAGGACTCGG AAGATGGGCC TTGCGTCTGT
    GACTTCAGCT GCCATAGCGT CCTGAGGAGC CCGGTCTGTG GCTCAGATGG GCTCACCTAT
    GGCACCGAGT GTGAGCTGAA GAAGGCCAGG TGTGAATCGC GGCGAGAACT GTACGTCACA
    GCACAGGGAG CCTGCCGTGG CCCCACCTTG GCCCCATTGC TACCTGCAGT TCCCCCACAC
    TGCACCCAGA CCCCCTATGG CTGCTGCCAG GACAACGTCA CTGCTGCCAG GGGTGTGGGC
    CTGGCTGGCT GCCCCAGCTC CTGCCAATGC AACCCACATG GCTCCTACAG TGGCACCTGT
    GACCCAACTA CGGGTCAGTG CTCTTGCCGC CCAGGCGTCG GGGGCCTCAA GTGTGACCGC
    TGTGAGCCTG GCTTCTGGAA CTTCCGTGGC ATTGTCACTG ATGGCCGGAG CGGCTGCACC
    CCCTGCAGCT GTGACCCCCG GGGCGCTGTG CGGGATGACT GCGAGCAGAT GACCGGGCTG
    TGTTCATGTA AGCCTGGGGT GGCTGGACCT AAGTGCGGGC AGTGTCCAGA TGGCCACGCT
    CTGGGCCCTG GCGGCTGTGA AACAGACTCC TTGGCGCCTG AGACGTGTGC TGAGATGCAC
    TGTGAGTTTG GGGCGTCCTG CGTGGAGGAG GCTGGCTCTG CACGCTGTGT GTGCCCAACA
    TCTACCTGTC CAGGGGCCAG TGCTACCAAG GTCTGTGGGT CAGATGGAGT CACCTACGGC
    AATGAGTGCC AGCTGAGGAC CATCGCCTGC CGCCAGGGCT TGGACATCTC CATCCAGAGC
    CTTGGCCCGT GCCAGGACTC TGTTCTCCTT GCAGAGACTG TTGCTCCTGG GGCCCGCCCA
    ACATCTGTAC CCAGGGCCAC CTCAGGGCTC AGCACGAGCA AGGCGCTGCC ACCCGCCCCC
    AGCACCCTCC CCCTGGCTCC CAGCAGCACC CCTCATAGCC GGCCCACGCC TCGACCCTCA
    CCAGGATCTC GGACCACTGC CAGCATTCCC AGGACTGTCA CGAGGCCTGT GCTGACCATG
    CCCCCCACAG CCCCCGCCAC GGCAGCCAAC CTCGCCACAT CTGCTTTTGG CGAGTCTGGC
    AGTGCTGACG GGAGCGGCGA TGAGGGGCTG AGTGGTGACC TGGAGGCCAG TGGGACTGGC
    TCAGGGGGAC TCGAGCCCCC TGAAGGTGGC AGTGCTGTGA CCCCTGAGCC ACCCATGGAG
    AGGGCTTCTT GCTACAACTC GCCTTTGGGC TGCTGCTCGG ATGGCAAGAC GCCCTCGCTG
    GACGCAGAGG GTTCCAACTG TCCTGCCACC AAGGCGTTCC AGGGTGTGTT GGAGCTTGAG
    GGCGTGGAGG GGCAAGAGCT GTTCTACACG CCGGAGATGG CTGACCCCAA GTCAGAGCTT
    TTTGGGGAGA CTGCTAGGAG CATCGAGAGC ACCCTGGATG ACCTCTTCCG GAACTCAGAG
    GTTAAGAAGG ACTTCCGAAG CGTCCGGCTG AGGGACTTGG GGCCTGGCAG CTCAGTCCGA
    GCCATTGTGG ATGTGCACTT TGACCCTGCC ACAACCTTTA GGGCGCCTGA TGTGGGCCAA
    GCCCTGCTCC GGCAGATACA GGCATCCCGG CGCCGGTCCC TGGGCGTACG GCGGCCACTG
    CAGGAGCACG TGCGCTTTAT GGACTTTGAC TGGTTTCCTG CGTTCTTCAC GGGAGCCACC
    ACAGGAGCAA CACCCGCCGC AGCCACGGCC AGAGCCACCA CGGTGGCCCA CCTGCCACCT
    GCTGGGACCC AGTGGGCCCT CTACCCCAGT CACACAAGCC GGCCTGTGGG CAGGACCACA
    GCTCCACCCA CCACACGCCA GCCTCCCACC ACTGCTCCCC GTCGCGTGCC CGGACGCCGG
    CCCTTGCCCC CAGGCACCCA GCAGCCCCCG AAGCCCTGCG ACTCCCAGCC CTGCCTCCAT
    GGGGGAACCT GCCAGGACCA GGACTCGGGT GGAGGCTTCA CCTGCAGCTG CCCAGTGGGC
    AGGAGGGGTG CCGTCTGTGA GAAGGCCCTG TACCTCTCGG TGCCAGCTTT CAATGGCCGC
    TCCTTCCTGG CCTTCCCCAC CCTCCGTGCC TACCACACGC TGCGCCTAGC ACTTGAGTTC
    CGGGCACTAG AGCCACAGGG CCTGCTGCTC TATAATGGCA ATGCCCAGGG CAAGGACTTC
    CTGGCATTGA CGCTGCTGGG GGGCCGTGTG CAGCTCAGGT TTGACACAGG TTCCGGGCCT
    GCAGTACTGA CCAGCTCAGT GCCTATAGAG CCTGGCCGCT GGCATCGCTT GGAGCTGTCT
    CGGCACTGGC GCCGGGGAAC ACTTTCAGTG GATGGTGAGC CCCTGGTGCT GGGCCACAGC
    CCCAGCGGTA CAGATGGTCT CAACCTGGAC ACAGACCTTT TTGTGGGCGG CATCCCTGAG
    GATCAGGCTT CTGTGGTACT CGAAAGGACG TCCATCAGCG TTGGCCTCAA GGGCTGCATC
    CGCCTGCTGG ATGTCAATAA TCAGCGTTTA GAGCTCAGCA GCTGGCCAGG GGCTGCAGCC
    CGAAGCTCCG GGGTGGGCGA GTGTGGAGAC CACCCCTGCC TGCCCAACCC CTGCCTCGAC
    GGGGCTCCGT GCCAGGCCCT GGAGGCTGGC ATGTTCCACT GTCAGTGCCC ACCTGGCCGC
    TTTGGTCCAA CCTGTAGTGG TGACAAGAAC CCCTGTGAGC CGAACCCCTG CCATGGGGCA
    GCCCCCTGCC GTGTGCTGCC TGATGGGGAG GCCAAGTGTG AATGCCCCCG AGGACGAGGG
    GGTGCCATCT GCCAGACAGT CTCGGAGCGG GATTCTTCCC AGCCCTTCCT GGCTGACTTC
    CATGGCTTCT CCTACCTGGA GCTGAAAGGG TTGCATACCT TTGAGCGGGA CCTGGGGGAG
    AAGCTGGCAC TGGAGGTGGT GTTCCTGGCC CGTGGGCCCA GTGGTTTGCT CCTCTACAAT
    GGGCAGAAAA CAGACGGCAA GGGGGACTTT GTGTCACTGG CGTTGCATGA CCGCTTCTTG
    GAATTCCGCT ATGACCTGGG CAAGGGAGCA GCGGTCATCA GGAGCAAGGA GCCGGTGGCC
    CTGGACACCT GGACCAGGGT CTCACTAGAG CGCAATGGCC GCAAGGGTGC CATGCGTGTC
    AGTGATGGGC CTCGCGTGCT GGGAGAGTCC CCGAAATCCC GCAAGGTGCC ACACACTGTC
    CTCAACCTGA AGGAGCCACT CTATGTTGGA GGGGCCCCTG ACTTCAGCAA ACTGGCCCGG
    GCAGCTGCGG TATTCTCTGG CTTCGATGGC GCCATCCAAC TGGTCTCCTT GAAAGGCCAC
    CAGCTGCTGT CCCAGGAGCA CGTGGTGCGG TCAGTGGATG TCTCATCTTT TGCCCACCAC
    CCTTGTACTC AGGCCTCGGG CCACCCATGC CTCAATGGGG CCTCCTGCCT CCCTCGAGAG
    GCCTCCTATG AGTGCCTGTG CCCCGGGGGC TTCTCAGGGC TTCACTGTGA GAAGGGGCTG
    ATTGAGAAGT CAGCAGGGGA TGTGGACGCC CTGGCCTTTG ACGGACAGAC CTACGTCGAG
    TACCTCAATG CCGTGACTGA GAGCGAACTG ACCAATGAGA TCCCAGCTCC TGATGCTCCG
    GATTCCGGGG CCCTTCCCAG TGAGAAGGCC CTGCAGAGCA ACCACTTTGA GCTGAGCCTG
    CGCACGGAAG CCACTCAGGG GCTGGTGCTG TGGAGCGGCA AGGCCACGGA GCGGGCCGAC
    TATGTCGCAC TGGCCATCGT GGATGGGCGC CTGCAGCTGA CCTATGACCT GGGCTCCCAG
    CCCGTCGTGC TGCGCTCCAC CGTGCCGGTC AACACCAACC GCTGGCTACG AGTCAGGGCA
    CATAGGGAAC AGAGGGAAGG TTCCCTTCAG GTGGGCAATG AGGCTCCCGT GACTGGCTCT
    TCCCCGCTGG GTGCCACACA GCTAGACACA GACGGAACTC TATGGCTGGG TGGCCTGGAG
    AAGCTGCCCA TAGGCCAGGC CCTGCCCAAG GCCTATGGCA CGGGCTTTGT GGGCTGCCTG
    CGGGATGTGG TGGTGGGGCA GCGCCCACTG CACCTGCTGG AGGATGCTGT CACCAAGCCA
    GAGCTCCGAC CCTGTGCCGC CCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005620422.1
    CDS747..6653
    Translation

    Target ORF information:

    RefSeq Version XM_005620365.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris agrin (AGRN), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005620365.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCCTCCGC CACCTTTGGA GCGGGACCCC CGGCAGCAGC GTGGGGTCTC CCTGCTGGTG 
    CGGTATTTTA TGATTCCCTG CAACGTCTGC CTGATTCTGC TGGCCACGTC CACGCTGGGC
    TTCGCGGTGC TGCTTTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC ACCAGCGCCC
    CCAACGCCTC CAGATGCATG CCGAGGAATG CTGTGTGGCT TCGGTGCTGT GTGTGAGCCC
    AGTGCAGGGG GTTCAGGCCG AGCCTCCTGC GTGTGCAAGA AGAGTGCCTG TCCCGCCGTG
    GTGGCGCCCG TGTGTGGCTC TGACGCCTCC ACCTACAGCA ATGAGTGTGA GCTGCAGCGG
    GCGCAGTGCA GCCAGCAGCG CCGCATCCGC CTACTCAGTC GCGGACCCTG TGGTTCCCGG
    GACCCCTGCT CCAATGTGAC ATGCAGCTTC GGCAGCACCT GTGTGCGCTC AGCAGATGGG
    CAGACAGCCA CATGCCTGTG CCCTGCCACC TGCCGGGGGC CCCCGGAGGG CCCCGTGTGT
    GGGAGCGACG GTGCTGACTA CCCCAGTGAG TGCCAGCTCC TGCGCCTCGC GTGCACTCGC
    CAGGAGAACA TCGTCAAGAA GTTTGACGGC CCTTGTGACC CCTGCCAGGG CACCCACCAT
    GACCTGAGCC GTGTCTGCCG CGTTCACCCA CGCACGCGGC ATCCTGAGAT GCTCCTCCGG
    CCAGAGAGTT GCCCTTCTCA GCCAGCACCT GTGTGCGGGG ATGATGGAGT CACCTATGAC
    AACGACTGCA TAATGGGCCG CACGGGAGCC ACCCGTGGCC TTGTCCTCCA GAAAGTGCGC
    TCTGGCCAGT GCCAGCCTCA AGACCAGTGT CCAGATGTGT GCAGGTTCAA TGCTGTGTGT
    CTGTCCCGCC GAGGCCGCCC CCGCTGCTCC TGTGACCGTG TCATCTGTGA TGGGGCTTAT
    AGACCCGTGT GTGCTCACGA CGGGCACACG TATGATAATG ACTGCTGGCG TCAGCAAGCT
    GAGTGTCGGC AGCAGCGCAC CATTCCCGCC AAGCACCAGG GCCCGTGTGA CCAGCCCCTG
    TCCCCGTGCC TTGGGGTGCG GTGTCCTTTT GGGGCAACAT GTGCCGTGAA GAATGGGGAG
    GCTGAGTGCG TGTGCCAGCA GGTGTGTTCG GGCATCTATG ATCCTGTGTG TGGCAGTGAT
    GGTGTCACAT ATGGCAGCAC ATGTGAACTG GAAGCCACGG CCTGCGCCCT TGGACGGGAG
    ATCCGGGTGG CTCGCAGAGG ACCCTGTGAC CGCTGTGGGC AGTGCCGCTT TGGAGCTCTG
    TGTGAGGCTG AGACTGGGCG CTGTGTGTGC CCCTCCGAGT GCGTGGCCTC GGCTCAACCT
    GTGTGCGGTT CTGATGGGCA CACGTATGCC AGCGAGTGTG AGCTGCACGT CCACGCCTGC
    ACACACCAGA TCAGCCTGCA TGTGGTCTCA GCTGGACACT GCCAGACCTG CGGAGACACA
    GTGTGTGCTT TCGGGGCAGT GTGCTTGGCT GGGCAATGTG TGTGTCCCCG ATGTGAGCGA
    CCCCCACCCG GGCCCGTGTG TGGCAGCGAT GGTGTCACCT ACGACAGTGC CTGTCATCTC
    CGAGAAGCTG CCTGCCAGCA ACAGACACAG ATTGAGGAGG CCCGGGCGGG GCCCTGTGAG
    CAGGCTGAGT GTGGCTCTGG AGGCTCTGGC TCCGGAGAGG ATGGCGAGTG TGAGCAGGAG
    CTGTGCCAGC AGCATGGCGG TGTCTGGGAT GAGGACTCGG AAGATGGGCC TTGCGTCTGT
    GACTTCAGCT GCCATAGCGT CCTGAGGAGC CCGGTCTGTG GCTCAGATGG GCTCACCTAT
    GGCACCGAGT GTGAGCTGAA GAAGGCCAGG TGTGAATCGC GGCGAGAACT GTACGTCACA
    GCACAGGGAG CCTGCCGTGG CCCCACCTTG GCCCCATTGC TACCTGCAGT TCCCCCACAC
    TGCACCCAGA CCCCCTATGG CTGCTGCCAG GACAACGTCA CTGCTGCCAG GGGTGTGGGC
    CTGGCTGGCT GCCCCAGCTC CTGCCAATGC AACCCACATG GCTCCTACAG TGGCACCTGT
    GACCCAACTA CGGGTCAGTG CTCTTGCCGC CCAGGCGTCG GGGGCCTCAA GTGTGACCGC
    TGTGAGCCTG GCTTCTGGAA CTTCCGTGGC ATTGTCACTG ATGGCCGGAG CGGCTGCACC
    CCCTGCAGCT GTGACCCCCG GGGCGCTGTG CGGGATGACT GCGAGCAGAT GACCGGGCTG
    TGTTCATGTA AGCCTGGGGT GGCTGGACCT AAGTGCGGGC AGTGTCCAGA TGGCCACGCT
    CTGGGCCCTG GCGGCTGTGA AACAGACTCC TTGGCGCCTG AGACGTGTGC TGAGATGCAC
    TGTGAGTTTG GGGCGTCCTG CGTGGAGGAG GCTGGCTCTG CACGCTGTGT GTGCCCAACA
    TCTACCTGTC CAGGGGCCAG TGCTACCAAG GTCTGTGGGT CAGATGGAGT CACCTACGGC
    AATGAGTGCC AGCTGAGGAC CATCGCCTGC CGCCAGGGCT TGGACATCTC CATCCAGAGC
    CTTGGCCCGT GCCAGGACTC TGTTCTCCTT GCAGAGACTG TTGCTCCTGG GGCCCGCCCA
    ACATCTGTAC CCAGGGCCAC CTCAGGGCTC AGCACGAGCA AGGCGCTGCC ACCCGCCCCC
    AGCACCCTCC CCCTGGCTCC CAGCAGCACC CCTCATAGCC GGCCCACGCC TCGACCCTCA
    CCAGGATCTC GGACCACTGC CAGCATTCCC AGGACTGTCA CGAGGCCTGT GCTGACCATG
    CCCCCCACAG CCCCCGCCAC GGCAGCCAAC CTCGCCACAT CTGCTTTTGG CGAGTCTGGC
    AGTGCTGACG GGAGCGGCGA TGAGGGGCTG AGTGGTGACC TGGAGGCCAG TGGGACTGGC
    TCAGGGGGAC TCGAGCCCCC TGAAGGTGGC AGTGCTGTGA CCCCTGAGCC ACCCATGGAG
    AGGGCTTCTT GCTACAACTC GCCTTTGGGC TGCTGCTCGG ATGGCAAGAC GCCCTCGCTG
    GACGCAGAGG GTTCCAACTG TCCTGCCACC AAGGCGTTCC AGGGTGTGTT GGAGCTTGAG
    GGCGTGGAGG GGCAAGAGCT GTTCTACACG CCGGAGATGG CTGACCCCAA GTCAGAGCTT
    TTTGGGGAGA CTGCTAGGAG CATCGAGAGC ACCCTGGATG ACCTCTTCCG GAACTCAGAG
    GTTAAGAAGG ACTTCCGAAG CGTCCGGCTG AGGGACTTGG GGCCTGGCAG CTCAGTCCGA
    GCCATTGTGG ATGTGCACTT TGACCCTGCC ACAACCTTTA GGGCGCCTGA TGTGGGCCAA
    GCCCTGCTCC GGCAGATACA GGCATCCCGG CGCCGGTCCC TGGGCGTACG GCGGCCACTG
    CAGGAGCACG TGCGCTTTAT GGACTTTGAC TGGTTTCCTG CGTTCTTCAC GGGAGCCACC
    ACAGGAGCAA CACCCGCCGC AGCCACGGCC AGAGCCACCA CGGTGGCCCA CCTGCCACCT
    GCTGGGACCC AGTGGGCCCT CTACCCCAGT CACACAAGCC GGCCTGTGGG CAGGACCACA
    GCTCCACCCA CCACACGCCA GCCTCCCACC ACTGCTCCCC GTCGCGTGCC CGGACGCCGG
    CCCTTGCCCC CAGGCACCCA GCAGCCCCCG AAGCCCTGCG ACTCCCAGCC CTGCCTCCAT
    GGGGGAACCT GCCAGGACCA GGACTCGGGT GGAGGCTTCA CCTGCAGCTG CCCAGTGGGC
    AGGAGGGGTG CCGTCTGTGA GAAGGCCCTG TACCTCTCGG TGCCAGCTTT CAATGGCCGC
    TCCTTCCTGG CCTTCCCCAC CCTCCGTGCC TACCACACGC TGCGCCTAGC ACTTGAGTTC
    CGGGCACTAG AGCCACAGGG CCTGCTGCTC TATAATGGCA ATGCCCAGGG CAAGGACTTC
    CTGGCATTGA CGCTGCTGGG GGGCCGTGTG CAGCTCAGGT TTGACACAGG TTCCGGGCCT
    GCAGTACTGA CCAGCTCAGT GCCTATAGAG CCTGGCCGCT GGCATCGCTT GGAGCTGTCT
    CGGCACTGGC GCCGGGGAAC ACTTTCAGTG GATGGTGAGC CCCTGGTGCT GGGCCACAGC
    CCCAGCGGTA CAGATGGTCT CAACCTGGAC ACAGACCTTT TTGTGGGCGG CATCCCTGAG
    GATCAGGCTT CTGTGGTACT CGAAAGGACG TCCATCAGCG TTGGCCTCAA GGGCTGCATC
    CGCCTGCTGG ATGTCAATAA TCAGCGTTTA GAGCTCAGCA GCTGGCCAGG GGCTGCAGCC
    CGAAGCTCCG GGGTGGGCGA GTGTGGAGAC CACCCCTGCC TGCCCAACCC CTGCCTCGAC
    GGGGCTCCGT GCCAGGCCCT GGAGGCTGGC ATGTTCCACT GTCAGTGCCC ACCTGGCCGC
    TTTGGTCCAA CCTGTAGTGG TGACAAGAAC CCCTGTGAGC CGAACCCCTG CCATGGGGCA
    GCCCCCTGCC GTGTGCTGCC TGATGGGGAG GCCAAGTGTG AATGCCCCCG AGGACGAGGG
    GGTGCCATCT GCCAGACAGT CTCGGAGCGG GATTCTTCCC AGCCCTTCCT GGCTGACTTC
    CATGGCTTCT CCTACCTGGA GCTGAAAGGG TTGCATACCT TTGAGCGGGA CCTGGGGGAG
    AAGCTGGCAC TGGAGGTGGT GTTCCTGGCC CGTGGGCCCA GTGGTTTGCT CCTCTACAAT
    GGGCAGAAAA CAGACGGCAA GGGGGACTTT GTGTCACTGG CGTTGCATGA CCGCTTCTTG
    GAATTCCGCT ATGACCTGGG CAAGGGAGCA GCGGTCATCA GGAGCAAGGA GCCGGTGGCC
    CTGGACACCT GGACCAGGGT CTCACTAGAG CGCAATGGCC GCAAGGGTGC CATGCGTGTC
    AGTGATGGGC CTCGCGTGCT GGGAGAGTCC CCGAAATCCC GCAAGGTGCC ACACACTGTC
    CTCAACCTGA AGGAGCCACT CTATGTTGGA GGGGCCCCTG ACTTCAGCAA ACTGGCCCGG
    GCAGCTGCGG TATTCTCTGG CTTCGATGGC GCCATCCAAC TGGTCTCCTT GAAAGGCCAC
    CAGCTGCTGT CCCAGGAGCA CGTGGTGCGG TCAGTGGATG TCTCATCTTT TGCCCACCAC
    CCTTGTACTC AGGCCTCGGG CCACCCATGC CTCAATGGGG CCTCCTGCCT CCCTCGAGAG
    GCCTCCTATG AGTGCCTGTG CCCCGGGGGC TTCTCAGGGC TTCACTGTGA GAAGGGGCTG
    ATTGAGAAGT CAGCAGGGGA TGTGGACGCC CTGGCCTTTG ACGGACAGAC CTACGTCGAG
    TACCTCAATG CCGTGACTGA GAGCGAACTG ACCAATGAGA TCCCAGCTCC TGATGCTCCG
    GATTCCGGGG CCCTTCCCAG TGAGAAGGCC CTGCAGAGCA ACCACTTTGA GCTGAGCCTG
    CGCACGGAAG CCACTCAGGG GCTGGTGCTG TGGAGCGGCA AGGCCACGGA GCGGGCCGAC
    TATGTCGCAC TGGCCATCGT GGATGGGCGC CTGCAGCTGA CCTATGACCT GGGCTCCCAG
    CCCGTCGTGC TGCGCTCCAC CGTGCCGGTC AACACCAACC GCTGGCTACG AGTCAGGGCA
    CATAGGGAAC AGAGGGAAGG TTCCCTTCAG GTGGGCAATG AGGCTCCCGT GACTGGCTCT
    TCCCCGCTGG GTGCCACACA GCTAGACACA GACGGAACTC TATGGCTGGG TGGCCTGGAG
    AAGCTGCCCA TAGGCCAGGC CCTGCCCAAG GCCTATGGCA CGGGCTTTGT GGGCTGCCTG
    CGGGATGTGG TGGTGGGGCA GCGCCCACTG CACCTGCTGG AGGATGCTGT CACCAAGCCA
    GAGCTCCGAC CCTGTGCCGC CCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06700
    Clone ID Related Accession (Same CDS sequence) XM_005620365.2 , XM_005620365.3
    Accession Version XM_005620365.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5907bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product agrin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005620365.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCCTCCGC CACCTTTGGA GCGGGACCCC CGGCAGCAGC GTGGGGTCTC CCTGCTGGTG 
    CGGTATTTTA TGATTCCCTG CAACGTCTGC CTGATTCTGC TGGCCACGTC CACGCTGGGC
    TTCGCGGTGC TGCTTTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC ACCAGCGCCC
    CCAACGCCTC CAGATGCATG CCGAGGAATG CTGTGTGGCT TCGGTGCTGT GTGTGAGCCC
    AGTGCAGGGG GTTCAGGCCG AGCCTCCTGC GTGTGCAAGA AGAGTGCCTG TCCCGCCGTG
    GTGGCGCCCG TGTGTGGCTC TGACGCCTCC ACCTACAGCA ATGAGTGTGA GCTGCAGCGG
    GCGCAGTGCA GCCAGCAGCG CCGCATCCGC CTACTCAGTC GCGGACCCTG TGGTTCCCGG
    GACCCCTGCT CCAATGTGAC ATGCAGCTTC GGCAGCACCT GTGTGCGCTC AGCAGATGGG
    CAGACAGCCA CATGCCTGTG CCCTGCCACC TGCCGGGGGC CCCCGGAGGG CCCCGTGTGT
    GGGAGCGACG GTGCTGACTA CCCCAGTGAG TGCCAGCTCC TGCGCCTCGC GTGCACTCGC
    CAGGAGAACA TCGTCAAGAA GTTTGACGGC CCTTGTGACC CCTGCCAGGG CACCCACCAT
    GACCTGAGCC GTGTCTGCCG CGTTCACCCA CGCACGCGGC ATCCTGAGAT GCTCCTCCGG
    CCAGAGAGTT GCCCTTCTCA GCCAGCACCT GTGTGCGGGG ATGATGGAGT CACCTATGAC
    AACGACTGCA TAATGGGCCG CACGGGAGCC ACCCGTGGCC TTGTCCTCCA GAAAGTGCGC
    TCTGGCCAGT GCCAGCCTCA AGACCAGTGT CCAGATGTGT GCAGGTTCAA TGCTGTGTGT
    CTGTCCCGCC GAGGCCGCCC CCGCTGCTCC TGTGACCGTG TCATCTGTGA TGGGGCTTAT
    AGACCCGTGT GTGCTCACGA CGGGCACACG TATGATAATG ACTGCTGGCG TCAGCAAGCT
    GAGTGTCGGC AGCAGCGCAC CATTCCCGCC AAGCACCAGG GCCCGTGTGA CCAGCCCCTG
    TCCCCGTGCC TTGGGGTGCG GTGTCCTTTT GGGGCAACAT GTGCCGTGAA GAATGGGGAG
    GCTGAGTGCG TGTGCCAGCA GGTGTGTTCG GGCATCTATG ATCCTGTGTG TGGCAGTGAT
    GGTGTCACAT ATGGCAGCAC ATGTGAACTG GAAGCCACGG CCTGCGCCCT TGGACGGGAG
    ATCCGGGTGG CTCGCAGAGG ACCCTGTGAC CGCTGTGGGC AGTGCCGCTT TGGAGCTCTG
    TGTGAGGCTG AGACTGGGCG CTGTGTGTGC CCCTCCGAGT GCGTGGCCTC GGCTCAACCT
    GTGTGCGGTT CTGATGGGCA CACGTATGCC AGCGAGTGTG AGCTGCACGT CCACGCCTGC
    ACACACCAGA TCAGCCTGCA TGTGGTCTCA GCTGGACACT GCCAGACCTG CGGAGACACA
    GTGTGTGCTT TCGGGGCAGT GTGCTTGGCT GGGCAATGTG TGTGTCCCCG ATGTGAGCGA
    CCCCCACCCG GGCCCGTGTG TGGCAGCGAT GGTGTCACCT ACGACAGTGC CTGTCATCTC
    CGAGAAGCTG CCTGCCAGCA ACAGACACAG ATTGAGGAGG CCCGGGCGGG GCCCTGTGAG
    CAGGCTGAGT GTGGCTCTGG AGGCTCTGGC TCCGGAGAGG ATGGCGAGTG TGAGCAGGAG
    CTGTGCCAGC AGCATGGCGG TGTCTGGGAT GAGGACTCGG AAGATGGGCC TTGCGTCTGT
    GACTTCAGCT GCCATAGCGT CCTGAGGAGC CCGGTCTGTG GCTCAGATGG GCTCACCTAT
    GGCACCGAGT GTGAGCTGAA GAAGGCCAGG TGTGAATCGC GGCGAGAACT GTACGTCACA
    GCACAGGGAG CCTGCCGTGG CCCCACCTTG GCCCCATTGC TACCTGCAGT TCCCCCACAC
    TGCACCCAGA CCCCCTATGG CTGCTGCCAG GACAACGTCA CTGCTGCCAG GGGTGTGGGC
    CTGGCTGGCT GCCCCAGCTC CTGCCAATGC AACCCACATG GCTCCTACAG TGGCACCTGT
    GACCCAACTA CGGGTCAGTG CTCTTGCCGC CCAGGCGTCG GGGGCCTCAA GTGTGACCGC
    TGTGAGCCTG GCTTCTGGAA CTTCCGTGGC ATTGTCACTG ATGGCCGGAG CGGCTGCACC
    CCCTGCAGCT GTGACCCCCG GGGCGCTGTG CGGGATGACT GCGAGCAGAT GACCGGGCTG
    TGTTCATGTA AGCCTGGGGT GGCTGGACCT AAGTGCGGGC AGTGTCCAGA TGGCCACGCT
    CTGGGCCCTG GCGGCTGTGA AACAGACTCC TTGGCGCCTG AGACGTGTGC TGAGATGCAC
    TGTGAGTTTG GGGCGTCCTG CGTGGAGGAG GCTGGCTCTG CACGCTGTGT GTGCCCAACA
    TCTACCTGTC CAGGGGCCAG TGCTACCAAG GTCTGTGGGT CAGATGGAGT CACCTACGGC
    AATGAGTGCC AGCTGAGGAC CATCGCCTGC CGCCAGGGCT TGGACATCTC CATCCAGAGC
    CTTGGCCCGT GCCAGGACTC TGTTCTCCTT GCAGAGACTG TTGCTCCTGG GGCCCGCCCA
    ACATCTGTAC CCAGGGCCAC CTCAGGGCTC AGCACGAGCA AGGCGCTGCC ACCCGCCCCC
    AGCACCCTCC CCCTGGCTCC CAGCAGCACC CCTCATAGCC GGCCCACGCC TCGACCCTCA
    CCAGGATCTC GGACCACTGC CAGCATTCCC AGGACTGTCA CGAGGCCTGT GCTGACCATG
    CCCCCCACAG CCCCCGCCAC GGCAGCCAAC CTCGCCACAT CTGCTTTTGG CGAGTCTGGC
    AGTGCTGACG GGAGCGGCGA TGAGGGGCTG AGTGGTGACC TGGAGGCCAG TGGGACTGGC
    TCAGGGGGAC TCGAGCCCCC TGAAGGTGGC AGTGCTGTGA CCCCTGAGCC ACCCATGGAG
    AGGGCTTCTT GCTACAACTC GCCTTTGGGC TGCTGCTCGG ATGGCAAGAC GCCCTCGCTG
    GACGCAGAGG GTTCCAACTG TCCTGCCACC AAGGCGTTCC AGGGTGTGTT GGAGCTTGAG
    GGCGTGGAGG GGCAAGAGCT GTTCTACACG CCGGAGATGG CTGACCCCAA GTCAGAGCTT
    TTTGGGGAGA CTGCTAGGAG CATCGAGAGC ACCCTGGATG ACCTCTTCCG GAACTCAGAG
    GTTAAGAAGG ACTTCCGAAG CGTCCGGCTG AGGGACTTGG GGCCTGGCAG CTCAGTCCGA
    GCCATTGTGG ATGTGCACTT TGACCCTGCC ACAACCTTTA GGGCGCCTGA TGTGGGCCAA
    GCCCTGCTCC GGCAGATACA GGCATCCCGG CGCCGGTCCC TGGGCGTACG GCGGCCACTG
    CAGGAGCACG TGCGCTTTAT GGACTTTGAC TGGTTTCCTG CGTTCTTCAC GGGAGCCACC
    ACAGGAGCAA CACCCGCCGC AGCCACGGCC AGAGCCACCA CGGTGGCCCA CCTGCCACCT
    GCTGGGACCC AGTGGGCCCT CTACCCCAGT CACACAAGCC GGCCTGTGGG CAGGACCACA
    GCTCCACCCA CCACACGCCA GCCTCCCACC ACTGCTCCCC GTCGCGTGCC CGGACGCCGG
    CCCTTGCCCC CAGGCACCCA GCAGCCCCCG AAGCCCTGCG ACTCCCAGCC CTGCCTCCAT
    GGGGGAACCT GCCAGGACCA GGACTCGGGT GGAGGCTTCA CCTGCAGCTG CCCAGTGGGC
    AGGAGGGGTG CCGTCTGTGA GAAGGCCCTG TACCTCTCGG TGCCAGCTTT CAATGGCCGC
    TCCTTCCTGG CCTTCCCCAC CCTCCGTGCC TACCACACGC TGCGCCTAGC ACTTGAGTTC
    CGGGCACTAG AGCCACAGGG CCTGCTGCTC TATAATGGCA ATGCCCAGGG CAAGGACTTC
    CTGGCATTGA CGCTGCTGGG GGGCCGTGTG CAGCTCAGGT TTGACACAGG TTCCGGGCCT
    GCAGTACTGA CCAGCTCAGT GCCTATAGAG CCTGGCCGCT GGCATCGCTT GGAGCTGTCT
    CGGCACTGGC GCCGGGGAAC ACTTTCAGTG GATGGTGAGC CCCTGGTGCT GGGCCACAGC
    CCCAGCGGTA CAGATGGTCT CAACCTGGAC ACAGACCTTT TTGTGGGCGG CATCCCTGAG
    GATCAGGCTT CTGTGGTACT CGAAAGGACG TCCATCAGCG TTGGCCTCAA GGGCTGCATC
    CGCCTGCTGG ATGTCAATAA TCAGCGTTTA GAGCTCAGCA GCTGGCCAGG GGCTGCAGCC
    CGAAGCTCCG GGGTGGGCGA GTGTGGAGAC CACCCCTGCC TGCCCAACCC CTGCCTCGAC
    GGGGCTCCGT GCCAGGCCCT GGAGGCTGGC ATGTTCCACT GTCAGTGCCC ACCTGGCCGC
    TTTGGTCCAA CCTGTAGTGG TGACAAGAAC CCCTGTGAGC CGAACCCCTG CCATGGGGCA
    GCCCCCTGCC GTGTGCTGCC TGATGGGGAG GCCAAGTGTG AATGCCCCCG AGGACGAGGG
    GGTGCCATCT GCCAGACAGT CTCGGAGCGG GATTCTTCCC AGCCCTTCCT GGCTGACTTC
    CATGGCTTCT CCTACCTGGA GCTGAAAGGG TTGCATACCT TTGAGCGGGA CCTGGGGGAG
    AAGCTGGCAC TGGAGGTGGT GTTCCTGGCC CGTGGGCCCA GTGGTTTGCT CCTCTACAAT
    GGGCAGAAAA CAGACGGCAA GGGGGACTTT GTGTCACTGG CGTTGCATGA CCGCTTCTTG
    GAATTCCGCT ATGACCTGGG CAAGGGAGCA GCGGTCATCA GGAGCAAGGA GCCGGTGGCC
    CTGGACACCT GGACCAGGGT CTCACTAGAG CGCAATGGCC GCAAGGGTGC CATGCGTGTC
    AGTGATGGGC CTCGCGTGCT GGGAGAGTCC CCGAAATCCC GCAAGGTGCC ACACACTGTC
    CTCAACCTGA AGGAGCCACT CTATGTTGGA GGGGCCCCTG ACTTCAGCAA ACTGGCCCGG
    GCAGCTGCGG TATTCTCTGG CTTCGATGGC GCCATCCAAC TGGTCTCCTT GAAAGGCCAC
    CAGCTGCTGT CCCAGGAGCA CGTGGTGCGG TCAGTGGATG TCTCATCTTT TGCCCACCAC
    CCTTGTACTC AGGCCTCGGG CCACCCATGC CTCAATGGGG CCTCCTGCCT CCCTCGAGAG
    GCCTCCTATG AGTGCCTGTG CCCCGGGGGC TTCTCAGGGC TTCACTGTGA GAAGGGGCTG
    ATTGAGAAGT CAGCAGGGGA TGTGGACGCC CTGGCCTTTG ACGGACAGAC CTACGTCGAG
    TACCTCAATG CCGTGACTGA GAGCGAACTG ACCAATGAGA TCCCAGCTCC TGATGCTCCG
    GATTCCGGGG CCCTTCCCAG TGAGAAGGCC CTGCAGAGCA ACCACTTTGA GCTGAGCCTG
    CGCACGGAAG CCACTCAGGG GCTGGTGCTG TGGAGCGGCA AGGCCACGGA GCGGGCCGAC
    TATGTCGCAC TGGCCATCGT GGATGGGCGC CTGCAGCTGA CCTATGACCT GGGCTCCCAG
    CCCGTCGTGC TGCGCTCCAC CGTGCCGGTC AACACCAACC GCTGGCTACG AGTCAGGGCA
    CATAGGGAAC AGAGGGAAGG TTCCCTTCAG GTGGGCAATG AGGCTCCCGT GACTGGCTCT
    TCCCCGCTGG GTGCCACACA GCTAGACACA GACGGAACTC TATGGCTGGG TGGCCTGGAG
    AAGCTGCCCA TAGGCCAGGC CCTGCCCAAG GCCTATGGCA CGGGCTTTGT GGGCTGCCTG
    CGGGATGTGG TGGTGGGGCA GCGCCCACTG CACCTGCTGG AGGATGCTGT CACCAAGCCA
    GAGCTCCGAC CCTGTGCCGC CCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005620422.1
    CDS255..6161
    Translation

    Target ORF information:

    RefSeq Version XM_005620365.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris agrin (AGRN), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005620365.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCCTCCGC CACCTTTGGA GCGGGACCCC CGGCAGCAGC GTGGGGTCTC CCTGCTGGTG 
    CGGTATTTTA TGATTCCCTG CAACGTCTGC CTGATTCTGC TGGCCACGTC CACGCTGGGC
    TTCGCGGTGC TGCTTTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC ACCAGCGCCC
    CCAACGCCTC CAGATGCATG CCGAGGAATG CTGTGTGGCT TCGGTGCTGT GTGTGAGCCC
    AGTGCAGGGG GTTCAGGCCG AGCCTCCTGC GTGTGCAAGA AGAGTGCCTG TCCCGCCGTG
    GTGGCGCCCG TGTGTGGCTC TGACGCCTCC ACCTACAGCA ATGAGTGTGA GCTGCAGCGG
    GCGCAGTGCA GCCAGCAGCG CCGCATCCGC CTACTCAGTC GCGGACCCTG TGGTTCCCGG
    GACCCCTGCT CCAATGTGAC ATGCAGCTTC GGCAGCACCT GTGTGCGCTC AGCAGATGGG
    CAGACAGCCA CATGCCTGTG CCCTGCCACC TGCCGGGGGC CCCCGGAGGG CCCCGTGTGT
    GGGAGCGACG GTGCTGACTA CCCCAGTGAG TGCCAGCTCC TGCGCCTCGC GTGCACTCGC
    CAGGAGAACA TCGTCAAGAA GTTTGACGGC CCTTGTGACC CCTGCCAGGG CACCCACCAT
    GACCTGAGCC GTGTCTGCCG CGTTCACCCA CGCACGCGGC ATCCTGAGAT GCTCCTCCGG
    CCAGAGAGTT GCCCTTCTCA GCCAGCACCT GTGTGCGGGG ATGATGGAGT CACCTATGAC
    AACGACTGCA TAATGGGCCG CACGGGAGCC ACCCGTGGCC TTGTCCTCCA GAAAGTGCGC
    TCTGGCCAGT GCCAGCCTCA AGACCAGTGT CCAGATGTGT GCAGGTTCAA TGCTGTGTGT
    CTGTCCCGCC GAGGCCGCCC CCGCTGCTCC TGTGACCGTG TCATCTGTGA TGGGGCTTAT
    AGACCCGTGT GTGCTCACGA CGGGCACACG TATGATAATG ACTGCTGGCG TCAGCAAGCT
    GAGTGTCGGC AGCAGCGCAC CATTCCCGCC AAGCACCAGG GCCCGTGTGA CCAGCCCCTG
    TCCCCGTGCC TTGGGGTGCG GTGTCCTTTT GGGGCAACAT GTGCCGTGAA GAATGGGGAG
    GCTGAGTGCG TGTGCCAGCA GGTGTGTTCG GGCATCTATG ATCCTGTGTG TGGCAGTGAT
    GGTGTCACAT ATGGCAGCAC ATGTGAACTG GAAGCCACGG CCTGCGCCCT TGGACGGGAG
    ATCCGGGTGG CTCGCAGAGG ACCCTGTGAC CGCTGTGGGC AGTGCCGCTT TGGAGCTCTG
    TGTGAGGCTG AGACTGGGCG CTGTGTGTGC CCCTCCGAGT GCGTGGCCTC GGCTCAACCT
    GTGTGCGGTT CTGATGGGCA CACGTATGCC AGCGAGTGTG AGCTGCACGT CCACGCCTGC
    ACACACCAGA TCAGCCTGCA TGTGGTCTCA GCTGGACACT GCCAGACCTG CGGAGACACA
    GTGTGTGCTT TCGGGGCAGT GTGCTTGGCT GGGCAATGTG TGTGTCCCCG ATGTGAGCGA
    CCCCCACCCG GGCCCGTGTG TGGCAGCGAT GGTGTCACCT ACGACAGTGC CTGTCATCTC
    CGAGAAGCTG CCTGCCAGCA ACAGACACAG ATTGAGGAGG CCCGGGCGGG GCCCTGTGAG
    CAGGCTGAGT GTGGCTCTGG AGGCTCTGGC TCCGGAGAGG ATGGCGAGTG TGAGCAGGAG
    CTGTGCCAGC AGCATGGCGG TGTCTGGGAT GAGGACTCGG AAGATGGGCC TTGCGTCTGT
    GACTTCAGCT GCCATAGCGT CCTGAGGAGC CCGGTCTGTG GCTCAGATGG GCTCACCTAT
    GGCACCGAGT GTGAGCTGAA GAAGGCCAGG TGTGAATCGC GGCGAGAACT GTACGTCACA
    GCACAGGGAG CCTGCCGTGG CCCCACCTTG GCCCCATTGC TACCTGCAGT TCCCCCACAC
    TGCACCCAGA CCCCCTATGG CTGCTGCCAG GACAACGTCA CTGCTGCCAG GGGTGTGGGC
    CTGGCTGGCT GCCCCAGCTC CTGCCAATGC AACCCACATG GCTCCTACAG TGGCACCTGT
    GACCCAACTA CGGGTCAGTG CTCTTGCCGC CCAGGCGTCG GGGGCCTCAA GTGTGACCGC
    TGTGAGCCTG GCTTCTGGAA CTTCCGTGGC ATTGTCACTG ATGGCCGGAG CGGCTGCACC
    CCCTGCAGCT GTGACCCCCG GGGCGCTGTG CGGGATGACT GCGAGCAGAT GACCGGGCTG
    TGTTCATGTA AGCCTGGGGT GGCTGGACCT AAGTGCGGGC AGTGTCCAGA TGGCCACGCT
    CTGGGCCCTG GCGGCTGTGA AACAGACTCC TTGGCGCCTG AGACGTGTGC TGAGATGCAC
    TGTGAGTTTG GGGCGTCCTG CGTGGAGGAG GCTGGCTCTG CACGCTGTGT GTGCCCAACA
    TCTACCTGTC CAGGGGCCAG TGCTACCAAG GTCTGTGGGT CAGATGGAGT CACCTACGGC
    AATGAGTGCC AGCTGAGGAC CATCGCCTGC CGCCAGGGCT TGGACATCTC CATCCAGAGC
    CTTGGCCCGT GCCAGGACTC TGTTCTCCTT GCAGAGACTG TTGCTCCTGG GGCCCGCCCA
    ACATCTGTAC CCAGGGCCAC CTCAGGGCTC AGCACGAGCA AGGCGCTGCC ACCCGCCCCC
    AGCACCCTCC CCCTGGCTCC CAGCAGCACC CCTCATAGCC GGCCCACGCC TCGACCCTCA
    CCAGGATCTC GGACCACTGC CAGCATTCCC AGGACTGTCA CGAGGCCTGT GCTGACCATG
    CCCCCCACAG CCCCCGCCAC GGCAGCCAAC CTCGCCACAT CTGCTTTTGG CGAGTCTGGC
    AGTGCTGACG GGAGCGGCGA TGAGGGGCTG AGTGGTGACC TGGAGGCCAG TGGGACTGGC
    TCAGGGGGAC TCGAGCCCCC TGAAGGTGGC AGTGCTGTGA CCCCTGAGCC ACCCATGGAG
    AGGGCTTCTT GCTACAACTC GCCTTTGGGC TGCTGCTCGG ATGGCAAGAC GCCCTCGCTG
    GACGCAGAGG GTTCCAACTG TCCTGCCACC AAGGCGTTCC AGGGTGTGTT GGAGCTTGAG
    GGCGTGGAGG GGCAAGAGCT GTTCTACACG CCGGAGATGG CTGACCCCAA GTCAGAGCTT
    TTTGGGGAGA CTGCTAGGAG CATCGAGAGC ACCCTGGATG ACCTCTTCCG GAACTCAGAG
    GTTAAGAAGG ACTTCCGAAG CGTCCGGCTG AGGGACTTGG GGCCTGGCAG CTCAGTCCGA
    GCCATTGTGG ATGTGCACTT TGACCCTGCC ACAACCTTTA GGGCGCCTGA TGTGGGCCAA
    GCCCTGCTCC GGCAGATACA GGCATCCCGG CGCCGGTCCC TGGGCGTACG GCGGCCACTG
    CAGGAGCACG TGCGCTTTAT GGACTTTGAC TGGTTTCCTG CGTTCTTCAC GGGAGCCACC
    ACAGGAGCAA CACCCGCCGC AGCCACGGCC AGAGCCACCA CGGTGGCCCA CCTGCCACCT
    GCTGGGACCC AGTGGGCCCT CTACCCCAGT CACACAAGCC GGCCTGTGGG CAGGACCACA
    GCTCCACCCA CCACACGCCA GCCTCCCACC ACTGCTCCCC GTCGCGTGCC CGGACGCCGG
    CCCTTGCCCC CAGGCACCCA GCAGCCCCCG AAGCCCTGCG ACTCCCAGCC CTGCCTCCAT
    GGGGGAACCT GCCAGGACCA GGACTCGGGT GGAGGCTTCA CCTGCAGCTG CCCAGTGGGC
    AGGAGGGGTG CCGTCTGTGA GAAGGCCCTG TACCTCTCGG TGCCAGCTTT CAATGGCCGC
    TCCTTCCTGG CCTTCCCCAC CCTCCGTGCC TACCACACGC TGCGCCTAGC ACTTGAGTTC
    CGGGCACTAG AGCCACAGGG CCTGCTGCTC TATAATGGCA ATGCCCAGGG CAAGGACTTC
    CTGGCATTGA CGCTGCTGGG GGGCCGTGTG CAGCTCAGGT TTGACACAGG TTCCGGGCCT
    GCAGTACTGA CCAGCTCAGT GCCTATAGAG CCTGGCCGCT GGCATCGCTT GGAGCTGTCT
    CGGCACTGGC GCCGGGGAAC ACTTTCAGTG GATGGTGAGC CCCTGGTGCT GGGCCACAGC
    CCCAGCGGTA CAGATGGTCT CAACCTGGAC ACAGACCTTT TTGTGGGCGG CATCCCTGAG
    GATCAGGCTT CTGTGGTACT CGAAAGGACG TCCATCAGCG TTGGCCTCAA GGGCTGCATC
    CGCCTGCTGG ATGTCAATAA TCAGCGTTTA GAGCTCAGCA GCTGGCCAGG GGCTGCAGCC
    CGAAGCTCCG GGGTGGGCGA GTGTGGAGAC CACCCCTGCC TGCCCAACCC CTGCCTCGAC
    GGGGCTCCGT GCCAGGCCCT GGAGGCTGGC ATGTTCCACT GTCAGTGCCC ACCTGGCCGC
    TTTGGTCCAA CCTGTAGTGG TGACAAGAAC CCCTGTGAGC CGAACCCCTG CCATGGGGCA
    GCCCCCTGCC GTGTGCTGCC TGATGGGGAG GCCAAGTGTG AATGCCCCCG AGGACGAGGG
    GGTGCCATCT GCCAGACAGT CTCGGAGCGG GATTCTTCCC AGCCCTTCCT GGCTGACTTC
    CATGGCTTCT CCTACCTGGA GCTGAAAGGG TTGCATACCT TTGAGCGGGA CCTGGGGGAG
    AAGCTGGCAC TGGAGGTGGT GTTCCTGGCC CGTGGGCCCA GTGGTTTGCT CCTCTACAAT
    GGGCAGAAAA CAGACGGCAA GGGGGACTTT GTGTCACTGG CGTTGCATGA CCGCTTCTTG
    GAATTCCGCT ATGACCTGGG CAAGGGAGCA GCGGTCATCA GGAGCAAGGA GCCGGTGGCC
    CTGGACACCT GGACCAGGGT CTCACTAGAG CGCAATGGCC GCAAGGGTGC CATGCGTGTC
    AGTGATGGGC CTCGCGTGCT GGGAGAGTCC CCGAAATCCC GCAAGGTGCC ACACACTGTC
    CTCAACCTGA AGGAGCCACT CTATGTTGGA GGGGCCCCTG ACTTCAGCAA ACTGGCCCGG
    GCAGCTGCGG TATTCTCTGG CTTCGATGGC GCCATCCAAC TGGTCTCCTT GAAAGGCCAC
    CAGCTGCTGT CCCAGGAGCA CGTGGTGCGG TCAGTGGATG TCTCATCTTT TGCCCACCAC
    CCTTGTACTC AGGCCTCGGG CCACCCATGC CTCAATGGGG CCTCCTGCCT CCCTCGAGAG
    GCCTCCTATG AGTGCCTGTG CCCCGGGGGC TTCTCAGGGC TTCACTGTGA GAAGGGGCTG
    ATTGAGAAGT CAGCAGGGGA TGTGGACGCC CTGGCCTTTG ACGGACAGAC CTACGTCGAG
    TACCTCAATG CCGTGACTGA GAGCGAACTG ACCAATGAGA TCCCAGCTCC TGATGCTCCG
    GATTCCGGGG CCCTTCCCAG TGAGAAGGCC CTGCAGAGCA ACCACTTTGA GCTGAGCCTG
    CGCACGGAAG CCACTCAGGG GCTGGTGCTG TGGAGCGGCA AGGCCACGGA GCGGGCCGAC
    TATGTCGCAC TGGCCATCGT GGATGGGCGC CTGCAGCTGA CCTATGACCT GGGCTCCCAG
    CCCGTCGTGC TGCGCTCCAC CGTGCCGGTC AACACCAACC GCTGGCTACG AGTCAGGGCA
    CATAGGGAAC AGAGGGAAGG TTCCCTTCAG GTGGGCAATG AGGCTCCCGT GACTGGCTCT
    TCCCCGCTGG GTGCCACACA GCTAGACACA GACGGAACTC TATGGCTGGG TGGCCTGGAG
    AAGCTGCCCA TAGGCCAGGC CCTGCCCAAG GCCTATGGCA CGGGCTTTGT GGGCTGCCTG
    CGGGATGTGG TGGTGGGGCA GCGCCCACTG CACCTGCTGG AGGATGCTGT CACCAAGCCA
    GAGCTCCGAC CCTGTGCCGC CCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06701
    Clone ID Related Accession (Same CDS sequence) XM_005620366.2 , XM_005620366.3
    Accession Version XM_005620366.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5889bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product agrin isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726058.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005620366.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCCTCCGC CACCTTTGGA GCGGGACCCC CGGCAGCAGC GTGGGGTCTC CCTGCTGGTG 
    CGGTATTTTA TGATTCCCTG CAACGTCTGC CTGATTCTGC TGGCCACGTC CACGCTGGGC
    TTCGCGGTGC TGCTTTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC ACCAGCGCCC
    CCAACGCCTC CAGATGCATG CCGAGGAATG CTGTGTGGCT TCGGTGCTGT GTGTGAGCCC
    AGTGCAGGGG GTTCAGGCCG AGCCTCCTGC GTGTGCAAGA AGAGTGCCTG TCCCGCCGTG
    GTGGCGCCCG TGTGTGGCTC TGACGCCTCC ACCTACAGCA ATGAGTGTGA GCTGCAGCGG
    GCGCAGTGCA GCCAGCAGCG CCGCATCCGC CTACTCAGTC GCGGACCCTG TGGTTCCCGG
    GACCCCTGCT CCAATGTGAC ATGCAGCTTC GGCAGCACCT GTGTGCGCTC AGCAGATGGG
    CAGACAGCCA CATGCCTGTG CCCTGCCACC TGCCGGGGGC CCCCGGAGGG CCCCGTGTGT
    GGGAGCGACG GTGCTGACTA CCCCAGTGAG TGCCAGCTCC TGCGCCTCGC GTGCACTCGC
    CAGGAGAACA TCGTCAAGAA GTTTGACGGC CCTTGTGACC CCTGCCAGGG CACCCACCAT
    GACCTGAGCC GTGTCTGCCG CGTTCACCCA CGCACGCGGC ATCCTGAGAT GCTCCTCCGG
    CCAGAGAGTT GCCCTTCTCA GCCAGCACCT GTGTGCGGGG ATGATGGAGT CACCTATGAC
    AACGACTGCA TAATGGGCCG CACGGGAGCC ACCCGTGGCC TTGTCCTCCA GAAAGTGCGC
    TCTGGCCAGT GCCAGCCTCA AGACCAGTGT CCAGATGTGT GCAGGTTCAA TGCTGTGTGT
    CTGTCCCGCC GAGGCCGCCC CCGCTGCTCC TGTGACCGTG TCATCTGTGA TGGGGCTTAT
    AGACCCGTGT GTGCTCACGA CGGGCACACG TATGATAATG ACTGCTGGCG TCAGCAAGCT
    GAGTGTCGGC AGCAGCGCAC CATTCCCGCC AAGCACCAGG GCCCGTGTGA CCAGCCCCTG
    TCCCCGTGCC TTGGGGTGCG GTGTCCTTTT GGGGCAACAT GTGCCGTGAA GAATGGGGAG
    GCTGAGTGCG TGTGCCAGCA GGTGTGTTCG GGCATCTATG ATCCTGTGTG TGGCAGTGAT
    GGTGTCACAT ATGGCAGCAC ATGTGAACTG GAAGCCACGG CCTGCGCCCT TGGACGGGAG
    ATCCGGGTGG CTCGCAGAGG ACCCTGTGAC CGCTGTGGGC AGTGCCGCTT TGGAGCTCTG
    TGTGAGGCTG AGACTGGGCG CTGTGTGTGC CCCTCCGAGT GCGTGGCCTC GGCTCAACCT
    GTGTGCGGTT CTGATGGGCA CACGTATGCC AGCGAGTGTG AGCTGCACGT CCACGCCTGC
    ACACACCAGA TCAGCCTGCA TGTGGTCTCA GCTGGACACT GCCAGACCTG CGGAGACACA
    GTGTGTGCTT TCGGGGCAGT GTGCTTGGCT GGGCAATGTG TGTGTCCCCG ATGTGAGCGA
    CCCCCACCCG GGCCCGTGTG TGGCAGCGAT GGTGTCACCT ACGACAGTGC CTGTCATCTC
    CGAGAAGCTG CCTGCCAGCA ACAGACACAG ATTGAGGAGG CCCGGGCGGG GCCCTGTGAG
    CAGGCTGAGT GTGGCTCTGG AGGCTCTGGC TCCGGAGAGG ATGGCGAGTG TGAGCAGGAG
    CTGTGCCAGC AGCATGGCGG TGTCTGGGAT GAGGACTCGG AAGATGGGCC TTGCGTCTGT
    GACTTCAGCT GCCATAGCGT CCTGAGGAGC CCGGTCTGTG GCTCAGATGG GCTCACCTAT
    GGCACCGAGT GTGAGCTGAA GAAGGCCAGG TGTGAATCGC GGCGAGAACT GTACGTCACA
    GCACAGGGAG CCTGCCGTGG CCCCACCTTG GCCCCATTGC TACCTGCAGT TCCCCCACAC
    TGCACCCAGA CCCCCTATGG CTGCTGCCAG GACAACGTCA CTGCTGCCAG GGGTGTGGGC
    CTGGCTGGCT GCCCCAGCTC CTGCCAATGC AACCCACATG GCTCCTACAG TGGCACCTGT
    GACCCAACTA CGGGTCAGTG CTCTTGCCGC CCAGGCGTCG GGGGCCTCAA GTGTGACCGC
    TGTGAGCCTG GCTTCTGGAA CTTCCGTGGC ATTGTCACTG ATGGCCGGAG CGGCTGCACC
    CCCTGCAGCT GTGACCCCCG GGGCGCTGTG CGGGATGACT GCGAGCAGAT GACCGGGCTG
    TGTTCATGTA AGCCTGGGGT GGCTGGACCT AAGTGCGGGC AGTGTCCAGA TGGCCACGCT
    CTGGGCCCTG GCGGCTGTGA AACAGACTCC TTGGCGCCTG AGACGTGTGC TGAGATGCAC
    TGTGAGTTTG GGGCGTCCTG CGTGGAGGAG GCTGGCTCTG CACGCTGTGT GTGCCCAACA
    TCTACCTGTC CAGGGGCCAG TGCTACCAAG GTCTGTGGGT CAGATGGAGT CACCTACGGC
    AATGAGTGCC AGCTGAGGAC CATCGCCTGC CGCCAGGGCT TGGACATCTC CATCCAGAGC
    CTTGGCCCGT GCCAGGAGAC TGTTGCTCCT GGGGCCCGCC CAACATCTGT ACCCAGGGCC
    ACCTCAGGGC TCAGCACGAG CAAGGCGCTG CCACCCGCCC CCAGCACCCT CCCCCTGGCT
    CCCAGCAGCA CCCCTCATAG CCGGCCCACG CCTCGACCCT CACCAGGATC TCGGACCACT
    GCCAGCATTC CCAGGACTGT CACGAGGCCT GTGCTGACCA TGCCCCCCAC AGCCCCCGCC
    ACGGCAGCCA ACCTCGCCAC ATCTGCTTTT GGCGAGTCTG GCAGTGCTGA CGGGAGCGGC
    GATGAGGGGC TGAGTGGTGA CCTGGAGGCC AGTGGGACTG GCTCAGGGGG ACTCGAGCCC
    CCTGAAGGTG GCAGTGCTGT GACCCCTGAG CCACCCATGG AGAGGGCTTC TTGCTACAAC
    TCGCCTTTGG GCTGCTGCTC GGATGGCAAG ACGCCCTCGC TGGACGCAGA GGGTTCCAAC
    TGTCCTGCCA CCAAGGCGTT CCAGGGTGTG TTGGAGCTTG AGGGCGTGGA GGGGCAAGAG
    CTGTTCTACA CGCCGGAGAT GGCTGACCCC AAGTCAGAGC TTTTTGGGGA GACTGCTAGG
    AGCATCGAGA GCACCCTGGA TGACCTCTTC CGGAACTCAG AGGTTAAGAA GGACTTCCGA
    AGCGTCCGGC TGAGGGACTT GGGGCCTGGC AGCTCAGTCC GAGCCATTGT GGATGTGCAC
    TTTGACCCTG CCACAACCTT TAGGGCGCCT GATGTGGGCC AAGCCCTGCT CCGGCAGATA
    CAGGCATCCC GGCGCCGGTC CCTGGGCGTA CGGCGGCCAC TGCAGGAGCA CGTGCGCTTT
    ATGGACTTTG ACTGGTTTCC TGCGTTCTTC ACGGGAGCCA CCACAGGAGC AACACCCGCC
    GCAGCCACGG CCAGAGCCAC CACGGTGGCC CACCTGCCAC CTGCTGGGAC CCAGTGGGCC
    CTCTACCCCA GTCACACAAG CCGGCCTGTG GGCAGGACCA CAGCTCCACC CACCACACGC
    CAGCCTCCCA CCACTGCTCC CCGTCGCGTG CCCGGACGCC GGCCCTTGCC CCCAGGCACC
    CAGCAGCCCC CGAAGCCCTG CGACTCCCAG CCCTGCCTCC ATGGGGGAAC CTGCCAGGAC
    CAGGACTCGG GTGGAGGCTT CACCTGCAGC TGCCCAGTGG GCAGGAGGGG TGCCGTCTGT
    GAGAAGGCCC TGTACCTCTC GGTGCCAGCT TTCAATGGCC GCTCCTTCCT GGCCTTCCCC
    ACCCTCCGTG CCTACCACAC GCTGCGCCTA GCACTTGAGT TCCGGGCACT AGAGCCACAG
    GGCCTGCTGC TCTATAATGG CAATGCCCAG GGCAAGGACT TCCTGGCATT GACGCTGCTG
    GGGGGCCGTG TGCAGCTCAG GTTTGACACA GGTTCCGGGC CTGCAGTACT GACCAGCTCA
    GTGCCTATAG AGCCTGGCCG CTGGCATCGC TTGGAGCTGT CTCGGCACTG GCGCCGGGGA
    ACACTTTCAG TGGATGGTGA GCCCCTGGTG CTGGGCCACA GCCCCAGCGG TACAGATGGT
    CTCAACCTGG ACACAGACCT TTTTGTGGGC GGCATCCCTG AGGATCAGGC TTCTGTGGTA
    CTCGAAAGGA CGTCCATCAG CGTTGGCCTC AAGGGCTGCA TCCGCCTGCT GGATGTCAAT
    AATCAGCGTT TAGAGCTCAG CAGCTGGCCA GGGGCTGCAG CCCGAAGCTC CGGGGTGGGC
    GAGTGTGGAG ACCACCCCTG CCTGCCCAAC CCCTGCCTCG ACGGGGCTCC GTGCCAGGCC
    CTGGAGGCTG GCATGTTCCA CTGTCAGTGC CCACCTGGCC GCTTTGGTCC AACCTGTAGT
    GGTGACAAGA ACCCCTGTGA GCCGAACCCC TGCCATGGGG CAGCCCCCTG CCGTGTGCTG
    CCTGATGGGG AGGCCAAGTG TGAATGCCCC CGAGGACGAG GGGGTGCCAT CTGCCAGACA
    GTCTCGGAGC GGGATTCTTC CCAGCCCTTC CTGGCTGACT TCCATGGCTT CTCCTACCTG
    GAGCTGAAAG GGTTGCATAC CTTTGAGCGG GACCTGGGGG AGAAGCTGGC ACTGGAGGTG
    GTGTTCCTGG CCCGTGGGCC CAGTGGTTTG CTCCTCTACA ATGGGCAGAA AACAGACGGC
    AAGGGGGACT TTGTGTCACT GGCGTTGCAT GACCGCTTCT TGGAATTCCG CTATGACCTG
    GGCAAGGGAG CAGCGGTCAT CAGGAGCAAG GAGCCGGTGG CCCTGGACAC CTGGACCAGG
    GTCTCACTAG AGCGCAATGG CCGCAAGGGT GCCATGCGTG TCAGTGATGG GCCTCGCGTG
    CTGGGAGAGT CCCCGAAATC CCGCAAGGTG CCACACACTG TCCTCAACCT GAAGGAGCCA
    CTCTATGTTG GAGGGGCCCC TGACTTCAGC AAACTGGCCC GGGCAGCTGC GGTATTCTCT
    GGCTTCGATG GCGCCATCCA ACTGGTCTCC TTGAAAGGCC ACCAGCTGCT GTCCCAGGAG
    CACGTGGTGC GGTCAGTGGA TGTCTCATCT TTTGCCCACC ACCCTTGTAC TCAGGCCTCG
    GGCCACCCAT GCCTCAATGG GGCCTCCTGC CTCCCTCGAG AGGCCTCCTA TGAGTGCCTG
    TGCCCCGGGG GCTTCTCAGG GCTTCACTGT GAGAAGGGGC TGATTGAGAA GTCAGCAGGG
    GATGTGGACG CCCTGGCCTT TGACGGACAG ACCTACGTCG AGTACCTCAA TGCCGTGACT
    GAGAGCGAAC TGACCAATGA GATCCCAGCT CCTGATGCTC CGGATTCCGG GGCCCTTCCC
    AGTGAGAAGG CCCTGCAGAG CAACCACTTT GAGCTGAGCC TGCGCACGGA AGCCACTCAG
    GGGCTGGTGC TGTGGAGCGG CAAGGCCACG GAGCGGGCCG ACTATGTCGC ACTGGCCATC
    GTGGATGGGC GCCTGCAGCT GACCTATGAC CTGGGCTCCC AGCCCGTCGT GCTGCGCTCC
    ACCGTGCCGG TCAACACCAA CCGCTGGCTA CGAGTCAGGG CACATAGGGA ACAGAGGGAA
    GGTTCCCTTC AGGTGGGCAA TGAGGCTCCC GTGACTGGCT CTTCCCCGCT GGGTGCCACA
    CAGCTAGACA CAGACGGAAC TCTATGGCTG GGTGGCCTGG AGAAGCTGCC CATAGGCCAG
    GCCCTGCCCA AGGCCTATGG CACGGGCTTT GTGGGCTGCC TGCGGGATGT GGTGGTGGGG
    CAGCGCCCAC TGCACCTGCT GGAGGATGCT GTCACCAAGC CAGAGCTCCG ACCCTGTGCC
    GCCCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005620423.1
    CDS747..6635
    Translation

    Target ORF information:

    RefSeq Version XM_005620366.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris agrin (AGRN), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005620366.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCCTCCGC CACCTTTGGA GCGGGACCCC CGGCAGCAGC GTGGGGTCTC CCTGCTGGTG 
    CGGTATTTTA TGATTCCCTG CAACGTCTGC CTGATTCTGC TGGCCACGTC CACGCTGGGC
    TTCGCGGTGC TGCTTTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC ACCAGCGCCC
    CCAACGCCTC CAGATGCATG CCGAGGAATG CTGTGTGGCT TCGGTGCTGT GTGTGAGCCC
    AGTGCAGGGG GTTCAGGCCG AGCCTCCTGC GTGTGCAAGA AGAGTGCCTG TCCCGCCGTG
    GTGGCGCCCG TGTGTGGCTC TGACGCCTCC ACCTACAGCA ATGAGTGTGA GCTGCAGCGG
    GCGCAGTGCA GCCAGCAGCG CCGCATCCGC CTACTCAGTC GCGGACCCTG TGGTTCCCGG
    GACCCCTGCT CCAATGTGAC ATGCAGCTTC GGCAGCACCT GTGTGCGCTC AGCAGATGGG
    CAGACAGCCA CATGCCTGTG CCCTGCCACC TGCCGGGGGC CCCCGGAGGG CCCCGTGTGT
    GGGAGCGACG GTGCTGACTA CCCCAGTGAG TGCCAGCTCC TGCGCCTCGC GTGCACTCGC
    CAGGAGAACA TCGTCAAGAA GTTTGACGGC CCTTGTGACC CCTGCCAGGG CACCCACCAT
    GACCTGAGCC GTGTCTGCCG CGTTCACCCA CGCACGCGGC ATCCTGAGAT GCTCCTCCGG
    CCAGAGAGTT GCCCTTCTCA GCCAGCACCT GTGTGCGGGG ATGATGGAGT CACCTATGAC
    AACGACTGCA TAATGGGCCG CACGGGAGCC ACCCGTGGCC TTGTCCTCCA GAAAGTGCGC
    TCTGGCCAGT GCCAGCCTCA AGACCAGTGT CCAGATGTGT GCAGGTTCAA TGCTGTGTGT
    CTGTCCCGCC GAGGCCGCCC CCGCTGCTCC TGTGACCGTG TCATCTGTGA TGGGGCTTAT
    AGACCCGTGT GTGCTCACGA CGGGCACACG TATGATAATG ACTGCTGGCG TCAGCAAGCT
    GAGTGTCGGC AGCAGCGCAC CATTCCCGCC AAGCACCAGG GCCCGTGTGA CCAGCCCCTG
    TCCCCGTGCC TTGGGGTGCG GTGTCCTTTT GGGGCAACAT GTGCCGTGAA GAATGGGGAG
    GCTGAGTGCG TGTGCCAGCA GGTGTGTTCG GGCATCTATG ATCCTGTGTG TGGCAGTGAT
    GGTGTCACAT ATGGCAGCAC ATGTGAACTG GAAGCCACGG CCTGCGCCCT TGGACGGGAG
    ATCCGGGTGG CTCGCAGAGG ACCCTGTGAC CGCTGTGGGC AGTGCCGCTT TGGAGCTCTG
    TGTGAGGCTG AGACTGGGCG CTGTGTGTGC CCCTCCGAGT GCGTGGCCTC GGCTCAACCT
    GTGTGCGGTT CTGATGGGCA CACGTATGCC AGCGAGTGTG AGCTGCACGT CCACGCCTGC
    ACACACCAGA TCAGCCTGCA TGTGGTCTCA GCTGGACACT GCCAGACCTG CGGAGACACA
    GTGTGTGCTT TCGGGGCAGT GTGCTTGGCT GGGCAATGTG TGTGTCCCCG ATGTGAGCGA
    CCCCCACCCG GGCCCGTGTG TGGCAGCGAT GGTGTCACCT ACGACAGTGC CTGTCATCTC
    CGAGAAGCTG CCTGCCAGCA ACAGACACAG ATTGAGGAGG CCCGGGCGGG GCCCTGTGAG
    CAGGCTGAGT GTGGCTCTGG AGGCTCTGGC TCCGGAGAGG ATGGCGAGTG TGAGCAGGAG
    CTGTGCCAGC AGCATGGCGG TGTCTGGGAT GAGGACTCGG AAGATGGGCC TTGCGTCTGT
    GACTTCAGCT GCCATAGCGT CCTGAGGAGC CCGGTCTGTG GCTCAGATGG GCTCACCTAT
    GGCACCGAGT GTGAGCTGAA GAAGGCCAGG TGTGAATCGC GGCGAGAACT GTACGTCACA
    GCACAGGGAG CCTGCCGTGG CCCCACCTTG GCCCCATTGC TACCTGCAGT TCCCCCACAC
    TGCACCCAGA CCCCCTATGG CTGCTGCCAG GACAACGTCA CTGCTGCCAG GGGTGTGGGC
    CTGGCTGGCT GCCCCAGCTC CTGCCAATGC AACCCACATG GCTCCTACAG TGGCACCTGT
    GACCCAACTA CGGGTCAGTG CTCTTGCCGC CCAGGCGTCG GGGGCCTCAA GTGTGACCGC
    TGTGAGCCTG GCTTCTGGAA CTTCCGTGGC ATTGTCACTG ATGGCCGGAG CGGCTGCACC
    CCCTGCAGCT GTGACCCCCG GGGCGCTGTG CGGGATGACT GCGAGCAGAT GACCGGGCTG
    TGTTCATGTA AGCCTGGGGT GGCTGGACCT AAGTGCGGGC AGTGTCCAGA TGGCCACGCT
    CTGGGCCCTG GCGGCTGTGA AACAGACTCC TTGGCGCCTG AGACGTGTGC TGAGATGCAC
    TGTGAGTTTG GGGCGTCCTG CGTGGAGGAG GCTGGCTCTG CACGCTGTGT GTGCCCAACA
    TCTACCTGTC CAGGGGCCAG TGCTACCAAG GTCTGTGGGT CAGATGGAGT CACCTACGGC
    AATGAGTGCC AGCTGAGGAC CATCGCCTGC CGCCAGGGCT TGGACATCTC CATCCAGAGC
    CTTGGCCCGT GCCAGGAGAC TGTTGCTCCT GGGGCCCGCC CAACATCTGT ACCCAGGGCC
    ACCTCAGGGC TCAGCACGAG CAAGGCGCTG CCACCCGCCC CCAGCACCCT CCCCCTGGCT
    CCCAGCAGCA CCCCTCATAG CCGGCCCACG CCTCGACCCT CACCAGGATC TCGGACCACT
    GCCAGCATTC CCAGGACTGT CACGAGGCCT GTGCTGACCA TGCCCCCCAC AGCCCCCGCC
    ACGGCAGCCA ACCTCGCCAC ATCTGCTTTT GGCGAGTCTG GCAGTGCTGA CGGGAGCGGC
    GATGAGGGGC TGAGTGGTGA CCTGGAGGCC AGTGGGACTG GCTCAGGGGG ACTCGAGCCC
    CCTGAAGGTG GCAGTGCTGT GACCCCTGAG CCACCCATGG AGAGGGCTTC TTGCTACAAC
    TCGCCTTTGG GCTGCTGCTC GGATGGCAAG ACGCCCTCGC TGGACGCAGA GGGTTCCAAC
    TGTCCTGCCA CCAAGGCGTT CCAGGGTGTG TTGGAGCTTG AGGGCGTGGA GGGGCAAGAG
    CTGTTCTACA CGCCGGAGAT GGCTGACCCC AAGTCAGAGC TTTTTGGGGA GACTGCTAGG
    AGCATCGAGA GCACCCTGGA TGACCTCTTC CGGAACTCAG AGGTTAAGAA GGACTTCCGA
    AGCGTCCGGC TGAGGGACTT GGGGCCTGGC AGCTCAGTCC GAGCCATTGT GGATGTGCAC
    TTTGACCCTG CCACAACCTT TAGGGCGCCT GATGTGGGCC AAGCCCTGCT CCGGCAGATA
    CAGGCATCCC GGCGCCGGTC CCTGGGCGTA CGGCGGCCAC TGCAGGAGCA CGTGCGCTTT
    ATGGACTTTG ACTGGTTTCC TGCGTTCTTC ACGGGAGCCA CCACAGGAGC AACACCCGCC
    GCAGCCACGG CCAGAGCCAC CACGGTGGCC CACCTGCCAC CTGCTGGGAC CCAGTGGGCC
    CTCTACCCCA GTCACACAAG CCGGCCTGTG GGCAGGACCA CAGCTCCACC CACCACACGC
    CAGCCTCCCA CCACTGCTCC CCGTCGCGTG CCCGGACGCC GGCCCTTGCC CCCAGGCACC
    CAGCAGCCCC CGAAGCCCTG CGACTCCCAG CCCTGCCTCC ATGGGGGAAC CTGCCAGGAC
    CAGGACTCGG GTGGAGGCTT CACCTGCAGC TGCCCAGTGG GCAGGAGGGG TGCCGTCTGT
    GAGAAGGCCC TGTACCTCTC GGTGCCAGCT TTCAATGGCC GCTCCTTCCT GGCCTTCCCC
    ACCCTCCGTG CCTACCACAC GCTGCGCCTA GCACTTGAGT TCCGGGCACT AGAGCCACAG
    GGCCTGCTGC TCTATAATGG CAATGCCCAG GGCAAGGACT TCCTGGCATT GACGCTGCTG
    GGGGGCCGTG TGCAGCTCAG GTTTGACACA GGTTCCGGGC CTGCAGTACT GACCAGCTCA
    GTGCCTATAG AGCCTGGCCG CTGGCATCGC TTGGAGCTGT CTCGGCACTG GCGCCGGGGA
    ACACTTTCAG TGGATGGTGA GCCCCTGGTG CTGGGCCACA GCCCCAGCGG TACAGATGGT
    CTCAACCTGG ACACAGACCT TTTTGTGGGC GGCATCCCTG AGGATCAGGC TTCTGTGGTA
    CTCGAAAGGA CGTCCATCAG CGTTGGCCTC AAGGGCTGCA TCCGCCTGCT GGATGTCAAT
    AATCAGCGTT TAGAGCTCAG CAGCTGGCCA GGGGCTGCAG CCCGAAGCTC CGGGGTGGGC
    GAGTGTGGAG ACCACCCCTG CCTGCCCAAC CCCTGCCTCG ACGGGGCTCC GTGCCAGGCC
    CTGGAGGCTG GCATGTTCCA CTGTCAGTGC CCACCTGGCC GCTTTGGTCC AACCTGTAGT
    GGTGACAAGA ACCCCTGTGA GCCGAACCCC TGCCATGGGG CAGCCCCCTG CCGTGTGCTG
    CCTGATGGGG AGGCCAAGTG TGAATGCCCC CGAGGACGAG GGGGTGCCAT CTGCCAGACA
    GTCTCGGAGC GGGATTCTTC CCAGCCCTTC CTGGCTGACT TCCATGGCTT CTCCTACCTG
    GAGCTGAAAG GGTTGCATAC CTTTGAGCGG GACCTGGGGG AGAAGCTGGC ACTGGAGGTG
    GTGTTCCTGG CCCGTGGGCC CAGTGGTTTG CTCCTCTACA ATGGGCAGAA AACAGACGGC
    AAGGGGGACT TTGTGTCACT GGCGTTGCAT GACCGCTTCT TGGAATTCCG CTATGACCTG
    GGCAAGGGAG CAGCGGTCAT CAGGAGCAAG GAGCCGGTGG CCCTGGACAC CTGGACCAGG
    GTCTCACTAG AGCGCAATGG CCGCAAGGGT GCCATGCGTG TCAGTGATGG GCCTCGCGTG
    CTGGGAGAGT CCCCGAAATC CCGCAAGGTG CCACACACTG TCCTCAACCT GAAGGAGCCA
    CTCTATGTTG GAGGGGCCCC TGACTTCAGC AAACTGGCCC GGGCAGCTGC GGTATTCTCT
    GGCTTCGATG GCGCCATCCA ACTGGTCTCC TTGAAAGGCC ACCAGCTGCT GTCCCAGGAG
    CACGTGGTGC GGTCAGTGGA TGTCTCATCT TTTGCCCACC ACCCTTGTAC TCAGGCCTCG
    GGCCACCCAT GCCTCAATGG GGCCTCCTGC CTCCCTCGAG AGGCCTCCTA TGAGTGCCTG
    TGCCCCGGGG GCTTCTCAGG GCTTCACTGT GAGAAGGGGC TGATTGAGAA GTCAGCAGGG
    GATGTGGACG CCCTGGCCTT TGACGGACAG ACCTACGTCG AGTACCTCAA TGCCGTGACT
    GAGAGCGAAC TGACCAATGA GATCCCAGCT CCTGATGCTC CGGATTCCGG GGCCCTTCCC
    AGTGAGAAGG CCCTGCAGAG CAACCACTTT GAGCTGAGCC TGCGCACGGA AGCCACTCAG
    GGGCTGGTGC TGTGGAGCGG CAAGGCCACG GAGCGGGCCG ACTATGTCGC ACTGGCCATC
    GTGGATGGGC GCCTGCAGCT GACCTATGAC CTGGGCTCCC AGCCCGTCGT GCTGCGCTCC
    ACCGTGCCGG TCAACACCAA CCGCTGGCTA CGAGTCAGGG CACATAGGGA ACAGAGGGAA
    GGTTCCCTTC AGGTGGGCAA TGAGGCTCCC GTGACTGGCT CTTCCCCGCT GGGTGCCACA
    CAGCTAGACA CAGACGGAAC TCTATGGCTG GGTGGCCTGG AGAAGCTGCC CATAGGCCAG
    GCCCTGCCCA AGGCCTATGG CACGGGCTTT GTGGGCTGCC TGCGGGATGT GGTGGTGGGG
    CAGCGCCCAC TGCACCTGCT GGAGGATGCT GTCACCAAGC CAGAGCTCCG ACCCTGTGCC
    GCCCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06701
    Clone ID Related Accession (Same CDS sequence) XM_005620366.2 , XM_005620366.3
    Accession Version XM_005620366.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5889bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product agrin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005620366.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCCTCCGC CACCTTTGGA GCGGGACCCC CGGCAGCAGC GTGGGGTCTC CCTGCTGGTG 
    CGGTATTTTA TGATTCCCTG CAACGTCTGC CTGATTCTGC TGGCCACGTC CACGCTGGGC
    TTCGCGGTGC TGCTTTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC ACCAGCGCCC
    CCAACGCCTC CAGATGCATG CCGAGGAATG CTGTGTGGCT TCGGTGCTGT GTGTGAGCCC
    AGTGCAGGGG GTTCAGGCCG AGCCTCCTGC GTGTGCAAGA AGAGTGCCTG TCCCGCCGTG
    GTGGCGCCCG TGTGTGGCTC TGACGCCTCC ACCTACAGCA ATGAGTGTGA GCTGCAGCGG
    GCGCAGTGCA GCCAGCAGCG CCGCATCCGC CTACTCAGTC GCGGACCCTG TGGTTCCCGG
    GACCCCTGCT CCAATGTGAC ATGCAGCTTC GGCAGCACCT GTGTGCGCTC AGCAGATGGG
    CAGACAGCCA CATGCCTGTG CCCTGCCACC TGCCGGGGGC CCCCGGAGGG CCCCGTGTGT
    GGGAGCGACG GTGCTGACTA CCCCAGTGAG TGCCAGCTCC TGCGCCTCGC GTGCACTCGC
    CAGGAGAACA TCGTCAAGAA GTTTGACGGC CCTTGTGACC CCTGCCAGGG CACCCACCAT
    GACCTGAGCC GTGTCTGCCG CGTTCACCCA CGCACGCGGC ATCCTGAGAT GCTCCTCCGG
    CCAGAGAGTT GCCCTTCTCA GCCAGCACCT GTGTGCGGGG ATGATGGAGT CACCTATGAC
    AACGACTGCA TAATGGGCCG CACGGGAGCC ACCCGTGGCC TTGTCCTCCA GAAAGTGCGC
    TCTGGCCAGT GCCAGCCTCA AGACCAGTGT CCAGATGTGT GCAGGTTCAA TGCTGTGTGT
    CTGTCCCGCC GAGGCCGCCC CCGCTGCTCC TGTGACCGTG TCATCTGTGA TGGGGCTTAT
    AGACCCGTGT GTGCTCACGA CGGGCACACG TATGATAATG ACTGCTGGCG TCAGCAAGCT
    GAGTGTCGGC AGCAGCGCAC CATTCCCGCC AAGCACCAGG GCCCGTGTGA CCAGCCCCTG
    TCCCCGTGCC TTGGGGTGCG GTGTCCTTTT GGGGCAACAT GTGCCGTGAA GAATGGGGAG
    GCTGAGTGCG TGTGCCAGCA GGTGTGTTCG GGCATCTATG ATCCTGTGTG TGGCAGTGAT
    GGTGTCACAT ATGGCAGCAC ATGTGAACTG GAAGCCACGG CCTGCGCCCT TGGACGGGAG
    ATCCGGGTGG CTCGCAGAGG ACCCTGTGAC CGCTGTGGGC AGTGCCGCTT TGGAGCTCTG
    TGTGAGGCTG AGACTGGGCG CTGTGTGTGC CCCTCCGAGT GCGTGGCCTC GGCTCAACCT
    GTGTGCGGTT CTGATGGGCA CACGTATGCC AGCGAGTGTG AGCTGCACGT CCACGCCTGC
    ACACACCAGA TCAGCCTGCA TGTGGTCTCA GCTGGACACT GCCAGACCTG CGGAGACACA
    GTGTGTGCTT TCGGGGCAGT GTGCTTGGCT GGGCAATGTG TGTGTCCCCG ATGTGAGCGA
    CCCCCACCCG GGCCCGTGTG TGGCAGCGAT GGTGTCACCT ACGACAGTGC CTGTCATCTC
    CGAGAAGCTG CCTGCCAGCA ACAGACACAG ATTGAGGAGG CCCGGGCGGG GCCCTGTGAG
    CAGGCTGAGT GTGGCTCTGG AGGCTCTGGC TCCGGAGAGG ATGGCGAGTG TGAGCAGGAG
    CTGTGCCAGC AGCATGGCGG TGTCTGGGAT GAGGACTCGG AAGATGGGCC TTGCGTCTGT
    GACTTCAGCT GCCATAGCGT CCTGAGGAGC CCGGTCTGTG GCTCAGATGG GCTCACCTAT
    GGCACCGAGT GTGAGCTGAA GAAGGCCAGG TGTGAATCGC GGCGAGAACT GTACGTCACA
    GCACAGGGAG CCTGCCGTGG CCCCACCTTG GCCCCATTGC TACCTGCAGT TCCCCCACAC
    TGCACCCAGA CCCCCTATGG CTGCTGCCAG GACAACGTCA CTGCTGCCAG GGGTGTGGGC
    CTGGCTGGCT GCCCCAGCTC CTGCCAATGC AACCCACATG GCTCCTACAG TGGCACCTGT
    GACCCAACTA CGGGTCAGTG CTCTTGCCGC CCAGGCGTCG GGGGCCTCAA GTGTGACCGC
    TGTGAGCCTG GCTTCTGGAA CTTCCGTGGC ATTGTCACTG ATGGCCGGAG CGGCTGCACC
    CCCTGCAGCT GTGACCCCCG GGGCGCTGTG CGGGATGACT GCGAGCAGAT GACCGGGCTG
    TGTTCATGTA AGCCTGGGGT GGCTGGACCT AAGTGCGGGC AGTGTCCAGA TGGCCACGCT
    CTGGGCCCTG GCGGCTGTGA AACAGACTCC TTGGCGCCTG AGACGTGTGC TGAGATGCAC
    TGTGAGTTTG GGGCGTCCTG CGTGGAGGAG GCTGGCTCTG CACGCTGTGT GTGCCCAACA
    TCTACCTGTC CAGGGGCCAG TGCTACCAAG GTCTGTGGGT CAGATGGAGT CACCTACGGC
    AATGAGTGCC AGCTGAGGAC CATCGCCTGC CGCCAGGGCT TGGACATCTC CATCCAGAGC
    CTTGGCCCGT GCCAGGAGAC TGTTGCTCCT GGGGCCCGCC CAACATCTGT ACCCAGGGCC
    ACCTCAGGGC TCAGCACGAG CAAGGCGCTG CCACCCGCCC CCAGCACCCT CCCCCTGGCT
    CCCAGCAGCA CCCCTCATAG CCGGCCCACG CCTCGACCCT CACCAGGATC TCGGACCACT
    GCCAGCATTC CCAGGACTGT CACGAGGCCT GTGCTGACCA TGCCCCCCAC AGCCCCCGCC
    ACGGCAGCCA ACCTCGCCAC ATCTGCTTTT GGCGAGTCTG GCAGTGCTGA CGGGAGCGGC
    GATGAGGGGC TGAGTGGTGA CCTGGAGGCC AGTGGGACTG GCTCAGGGGG ACTCGAGCCC
    CCTGAAGGTG GCAGTGCTGT GACCCCTGAG CCACCCATGG AGAGGGCTTC TTGCTACAAC
    TCGCCTTTGG GCTGCTGCTC GGATGGCAAG ACGCCCTCGC TGGACGCAGA GGGTTCCAAC
    TGTCCTGCCA CCAAGGCGTT CCAGGGTGTG TTGGAGCTTG AGGGCGTGGA GGGGCAAGAG
    CTGTTCTACA CGCCGGAGAT GGCTGACCCC AAGTCAGAGC TTTTTGGGGA GACTGCTAGG
    AGCATCGAGA GCACCCTGGA TGACCTCTTC CGGAACTCAG AGGTTAAGAA GGACTTCCGA
    AGCGTCCGGC TGAGGGACTT GGGGCCTGGC AGCTCAGTCC GAGCCATTGT GGATGTGCAC
    TTTGACCCTG CCACAACCTT TAGGGCGCCT GATGTGGGCC AAGCCCTGCT CCGGCAGATA
    CAGGCATCCC GGCGCCGGTC CCTGGGCGTA CGGCGGCCAC TGCAGGAGCA CGTGCGCTTT
    ATGGACTTTG ACTGGTTTCC TGCGTTCTTC ACGGGAGCCA CCACAGGAGC AACACCCGCC
    GCAGCCACGG CCAGAGCCAC CACGGTGGCC CACCTGCCAC CTGCTGGGAC CCAGTGGGCC
    CTCTACCCCA GTCACACAAG CCGGCCTGTG GGCAGGACCA CAGCTCCACC CACCACACGC
    CAGCCTCCCA CCACTGCTCC CCGTCGCGTG CCCGGACGCC GGCCCTTGCC CCCAGGCACC
    CAGCAGCCCC CGAAGCCCTG CGACTCCCAG CCCTGCCTCC ATGGGGGAAC CTGCCAGGAC
    CAGGACTCGG GTGGAGGCTT CACCTGCAGC TGCCCAGTGG GCAGGAGGGG TGCCGTCTGT
    GAGAAGGCCC TGTACCTCTC GGTGCCAGCT TTCAATGGCC GCTCCTTCCT GGCCTTCCCC
    ACCCTCCGTG CCTACCACAC GCTGCGCCTA GCACTTGAGT TCCGGGCACT AGAGCCACAG
    GGCCTGCTGC TCTATAATGG CAATGCCCAG GGCAAGGACT TCCTGGCATT GACGCTGCTG
    GGGGGCCGTG TGCAGCTCAG GTTTGACACA GGTTCCGGGC CTGCAGTACT GACCAGCTCA
    GTGCCTATAG AGCCTGGCCG CTGGCATCGC TTGGAGCTGT CTCGGCACTG GCGCCGGGGA
    ACACTTTCAG TGGATGGTGA GCCCCTGGTG CTGGGCCACA GCCCCAGCGG TACAGATGGT
    CTCAACCTGG ACACAGACCT TTTTGTGGGC GGCATCCCTG AGGATCAGGC TTCTGTGGTA
    CTCGAAAGGA CGTCCATCAG CGTTGGCCTC AAGGGCTGCA TCCGCCTGCT GGATGTCAAT
    AATCAGCGTT TAGAGCTCAG CAGCTGGCCA GGGGCTGCAG CCCGAAGCTC CGGGGTGGGC
    GAGTGTGGAG ACCACCCCTG CCTGCCCAAC CCCTGCCTCG ACGGGGCTCC GTGCCAGGCC
    CTGGAGGCTG GCATGTTCCA CTGTCAGTGC CCACCTGGCC GCTTTGGTCC AACCTGTAGT
    GGTGACAAGA ACCCCTGTGA GCCGAACCCC TGCCATGGGG CAGCCCCCTG CCGTGTGCTG
    CCTGATGGGG AGGCCAAGTG TGAATGCCCC CGAGGACGAG GGGGTGCCAT CTGCCAGACA
    GTCTCGGAGC GGGATTCTTC CCAGCCCTTC CTGGCTGACT TCCATGGCTT CTCCTACCTG
    GAGCTGAAAG GGTTGCATAC CTTTGAGCGG GACCTGGGGG AGAAGCTGGC ACTGGAGGTG
    GTGTTCCTGG CCCGTGGGCC CAGTGGTTTG CTCCTCTACA ATGGGCAGAA AACAGACGGC
    AAGGGGGACT TTGTGTCACT GGCGTTGCAT GACCGCTTCT TGGAATTCCG CTATGACCTG
    GGCAAGGGAG CAGCGGTCAT CAGGAGCAAG GAGCCGGTGG CCCTGGACAC CTGGACCAGG
    GTCTCACTAG AGCGCAATGG CCGCAAGGGT GCCATGCGTG TCAGTGATGG GCCTCGCGTG
    CTGGGAGAGT CCCCGAAATC CCGCAAGGTG CCACACACTG TCCTCAACCT GAAGGAGCCA
    CTCTATGTTG GAGGGGCCCC TGACTTCAGC AAACTGGCCC GGGCAGCTGC GGTATTCTCT
    GGCTTCGATG GCGCCATCCA ACTGGTCTCC TTGAAAGGCC ACCAGCTGCT GTCCCAGGAG
    CACGTGGTGC GGTCAGTGGA TGTCTCATCT TTTGCCCACC ACCCTTGTAC TCAGGCCTCG
    GGCCACCCAT GCCTCAATGG GGCCTCCTGC CTCCCTCGAG AGGCCTCCTA TGAGTGCCTG
    TGCCCCGGGG GCTTCTCAGG GCTTCACTGT GAGAAGGGGC TGATTGAGAA GTCAGCAGGG
    GATGTGGACG CCCTGGCCTT TGACGGACAG ACCTACGTCG AGTACCTCAA TGCCGTGACT
    GAGAGCGAAC TGACCAATGA GATCCCAGCT CCTGATGCTC CGGATTCCGG GGCCCTTCCC
    AGTGAGAAGG CCCTGCAGAG CAACCACTTT GAGCTGAGCC TGCGCACGGA AGCCACTCAG
    GGGCTGGTGC TGTGGAGCGG CAAGGCCACG GAGCGGGCCG ACTATGTCGC ACTGGCCATC
    GTGGATGGGC GCCTGCAGCT GACCTATGAC CTGGGCTCCC AGCCCGTCGT GCTGCGCTCC
    ACCGTGCCGG TCAACACCAA CCGCTGGCTA CGAGTCAGGG CACATAGGGA ACAGAGGGAA
    GGTTCCCTTC AGGTGGGCAA TGAGGCTCCC GTGACTGGCT CTTCCCCGCT GGGTGCCACA
    CAGCTAGACA CAGACGGAAC TCTATGGCTG GGTGGCCTGG AGAAGCTGCC CATAGGCCAG
    GCCCTGCCCA AGGCCTATGG CACGGGCTTT GTGGGCTGCC TGCGGGATGT GGTGGTGGGG
    CAGCGCCCAC TGCACCTGCT GGAGGATGCT GTCACCAAGC CAGAGCTCCG ACCCTGTGCC
    GCCCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005620423.1
    CDS254..6142
    Translation

    Target ORF information:

    RefSeq Version XM_005620366.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris agrin (AGRN), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005620366.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCCTCCGC CACCTTTGGA GCGGGACCCC CGGCAGCAGC GTGGGGTCTC CCTGCTGGTG 
    CGGTATTTTA TGATTCCCTG CAACGTCTGC CTGATTCTGC TGGCCACGTC CACGCTGGGC
    TTCGCGGTGC TGCTTTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC ACCAGCGCCC
    CCAACGCCTC CAGATGCATG CCGAGGAATG CTGTGTGGCT TCGGTGCTGT GTGTGAGCCC
    AGTGCAGGGG GTTCAGGCCG AGCCTCCTGC GTGTGCAAGA AGAGTGCCTG TCCCGCCGTG
    GTGGCGCCCG TGTGTGGCTC TGACGCCTCC ACCTACAGCA ATGAGTGTGA GCTGCAGCGG
    GCGCAGTGCA GCCAGCAGCG CCGCATCCGC CTACTCAGTC GCGGACCCTG TGGTTCCCGG
    GACCCCTGCT CCAATGTGAC ATGCAGCTTC GGCAGCACCT GTGTGCGCTC AGCAGATGGG
    CAGACAGCCA CATGCCTGTG CCCTGCCACC TGCCGGGGGC CCCCGGAGGG CCCCGTGTGT
    GGGAGCGACG GTGCTGACTA CCCCAGTGAG TGCCAGCTCC TGCGCCTCGC GTGCACTCGC
    CAGGAGAACA TCGTCAAGAA GTTTGACGGC CCTTGTGACC CCTGCCAGGG CACCCACCAT
    GACCTGAGCC GTGTCTGCCG CGTTCACCCA CGCACGCGGC ATCCTGAGAT GCTCCTCCGG
    CCAGAGAGTT GCCCTTCTCA GCCAGCACCT GTGTGCGGGG ATGATGGAGT CACCTATGAC
    AACGACTGCA TAATGGGCCG CACGGGAGCC ACCCGTGGCC TTGTCCTCCA GAAAGTGCGC
    TCTGGCCAGT GCCAGCCTCA AGACCAGTGT CCAGATGTGT GCAGGTTCAA TGCTGTGTGT
    CTGTCCCGCC GAGGCCGCCC CCGCTGCTCC TGTGACCGTG TCATCTGTGA TGGGGCTTAT
    AGACCCGTGT GTGCTCACGA CGGGCACACG TATGATAATG ACTGCTGGCG TCAGCAAGCT
    GAGTGTCGGC AGCAGCGCAC CATTCCCGCC AAGCACCAGG GCCCGTGTGA CCAGCCCCTG
    TCCCCGTGCC TTGGGGTGCG GTGTCCTTTT GGGGCAACAT GTGCCGTGAA GAATGGGGAG
    GCTGAGTGCG TGTGCCAGCA GGTGTGTTCG GGCATCTATG ATCCTGTGTG TGGCAGTGAT
    GGTGTCACAT ATGGCAGCAC ATGTGAACTG GAAGCCACGG CCTGCGCCCT TGGACGGGAG
    ATCCGGGTGG CTCGCAGAGG ACCCTGTGAC CGCTGTGGGC AGTGCCGCTT TGGAGCTCTG
    TGTGAGGCTG AGACTGGGCG CTGTGTGTGC CCCTCCGAGT GCGTGGCCTC GGCTCAACCT
    GTGTGCGGTT CTGATGGGCA CACGTATGCC AGCGAGTGTG AGCTGCACGT CCACGCCTGC
    ACACACCAGA TCAGCCTGCA TGTGGTCTCA GCTGGACACT GCCAGACCTG CGGAGACACA
    GTGTGTGCTT TCGGGGCAGT GTGCTTGGCT GGGCAATGTG TGTGTCCCCG ATGTGAGCGA
    CCCCCACCCG GGCCCGTGTG TGGCAGCGAT GGTGTCACCT ACGACAGTGC CTGTCATCTC
    CGAGAAGCTG CCTGCCAGCA ACAGACACAG ATTGAGGAGG CCCGGGCGGG GCCCTGTGAG
    CAGGCTGAGT GTGGCTCTGG AGGCTCTGGC TCCGGAGAGG ATGGCGAGTG TGAGCAGGAG
    CTGTGCCAGC AGCATGGCGG TGTCTGGGAT GAGGACTCGG AAGATGGGCC TTGCGTCTGT
    GACTTCAGCT GCCATAGCGT CCTGAGGAGC CCGGTCTGTG GCTCAGATGG GCTCACCTAT
    GGCACCGAGT GTGAGCTGAA GAAGGCCAGG TGTGAATCGC GGCGAGAACT GTACGTCACA
    GCACAGGGAG CCTGCCGTGG CCCCACCTTG GCCCCATTGC TACCTGCAGT TCCCCCACAC
    TGCACCCAGA CCCCCTATGG CTGCTGCCAG GACAACGTCA CTGCTGCCAG GGGTGTGGGC
    CTGGCTGGCT GCCCCAGCTC CTGCCAATGC AACCCACATG GCTCCTACAG TGGCACCTGT
    GACCCAACTA CGGGTCAGTG CTCTTGCCGC CCAGGCGTCG GGGGCCTCAA GTGTGACCGC
    TGTGAGCCTG GCTTCTGGAA CTTCCGTGGC ATTGTCACTG ATGGCCGGAG CGGCTGCACC
    CCCTGCAGCT GTGACCCCCG GGGCGCTGTG CGGGATGACT GCGAGCAGAT GACCGGGCTG
    TGTTCATGTA AGCCTGGGGT GGCTGGACCT AAGTGCGGGC AGTGTCCAGA TGGCCACGCT
    CTGGGCCCTG GCGGCTGTGA AACAGACTCC TTGGCGCCTG AGACGTGTGC TGAGATGCAC
    TGTGAGTTTG GGGCGTCCTG CGTGGAGGAG GCTGGCTCTG CACGCTGTGT GTGCCCAACA
    TCTACCTGTC CAGGGGCCAG TGCTACCAAG GTCTGTGGGT CAGATGGAGT CACCTACGGC
    AATGAGTGCC AGCTGAGGAC CATCGCCTGC CGCCAGGGCT TGGACATCTC CATCCAGAGC
    CTTGGCCCGT GCCAGGAGAC TGTTGCTCCT GGGGCCCGCC CAACATCTGT ACCCAGGGCC
    ACCTCAGGGC TCAGCACGAG CAAGGCGCTG CCACCCGCCC CCAGCACCCT CCCCCTGGCT
    CCCAGCAGCA CCCCTCATAG CCGGCCCACG CCTCGACCCT CACCAGGATC TCGGACCACT
    GCCAGCATTC CCAGGACTGT CACGAGGCCT GTGCTGACCA TGCCCCCCAC AGCCCCCGCC
    ACGGCAGCCA ACCTCGCCAC ATCTGCTTTT GGCGAGTCTG GCAGTGCTGA CGGGAGCGGC
    GATGAGGGGC TGAGTGGTGA CCTGGAGGCC AGTGGGACTG GCTCAGGGGG ACTCGAGCCC
    CCTGAAGGTG GCAGTGCTGT GACCCCTGAG CCACCCATGG AGAGGGCTTC TTGCTACAAC
    TCGCCTTTGG GCTGCTGCTC GGATGGCAAG ACGCCCTCGC TGGACGCAGA GGGTTCCAAC
    TGTCCTGCCA CCAAGGCGTT CCAGGGTGTG TTGGAGCTTG AGGGCGTGGA GGGGCAAGAG
    CTGTTCTACA CGCCGGAGAT GGCTGACCCC AAGTCAGAGC TTTTTGGGGA GACTGCTAGG
    AGCATCGAGA GCACCCTGGA TGACCTCTTC CGGAACTCAG AGGTTAAGAA GGACTTCCGA
    AGCGTCCGGC TGAGGGACTT GGGGCCTGGC AGCTCAGTCC GAGCCATTGT GGATGTGCAC
    TTTGACCCTG CCACAACCTT TAGGGCGCCT GATGTGGGCC AAGCCCTGCT CCGGCAGATA
    CAGGCATCCC GGCGCCGGTC CCTGGGCGTA CGGCGGCCAC TGCAGGAGCA CGTGCGCTTT
    ATGGACTTTG ACTGGTTTCC TGCGTTCTTC ACGGGAGCCA CCACAGGAGC AACACCCGCC
    GCAGCCACGG CCAGAGCCAC CACGGTGGCC CACCTGCCAC CTGCTGGGAC CCAGTGGGCC
    CTCTACCCCA GTCACACAAG CCGGCCTGTG GGCAGGACCA CAGCTCCACC CACCACACGC
    CAGCCTCCCA CCACTGCTCC CCGTCGCGTG CCCGGACGCC GGCCCTTGCC CCCAGGCACC
    CAGCAGCCCC CGAAGCCCTG CGACTCCCAG CCCTGCCTCC ATGGGGGAAC CTGCCAGGAC
    CAGGACTCGG GTGGAGGCTT CACCTGCAGC TGCCCAGTGG GCAGGAGGGG TGCCGTCTGT
    GAGAAGGCCC TGTACCTCTC GGTGCCAGCT TTCAATGGCC GCTCCTTCCT GGCCTTCCCC
    ACCCTCCGTG CCTACCACAC GCTGCGCCTA GCACTTGAGT TCCGGGCACT AGAGCCACAG
    GGCCTGCTGC TCTATAATGG CAATGCCCAG GGCAAGGACT TCCTGGCATT GACGCTGCTG
    GGGGGCCGTG TGCAGCTCAG GTTTGACACA GGTTCCGGGC CTGCAGTACT GACCAGCTCA
    GTGCCTATAG AGCCTGGCCG CTGGCATCGC TTGGAGCTGT CTCGGCACTG GCGCCGGGGA
    ACACTTTCAG TGGATGGTGA GCCCCTGGTG CTGGGCCACA GCCCCAGCGG TACAGATGGT
    CTCAACCTGG ACACAGACCT TTTTGTGGGC GGCATCCCTG AGGATCAGGC TTCTGTGGTA
    CTCGAAAGGA CGTCCATCAG CGTTGGCCTC AAGGGCTGCA TCCGCCTGCT GGATGTCAAT
    AATCAGCGTT TAGAGCTCAG CAGCTGGCCA GGGGCTGCAG CCCGAAGCTC CGGGGTGGGC
    GAGTGTGGAG ACCACCCCTG CCTGCCCAAC CCCTGCCTCG ACGGGGCTCC GTGCCAGGCC
    CTGGAGGCTG GCATGTTCCA CTGTCAGTGC CCACCTGGCC GCTTTGGTCC AACCTGTAGT
    GGTGACAAGA ACCCCTGTGA GCCGAACCCC TGCCATGGGG CAGCCCCCTG CCGTGTGCTG
    CCTGATGGGG AGGCCAAGTG TGAATGCCCC CGAGGACGAG GGGGTGCCAT CTGCCAGACA
    GTCTCGGAGC GGGATTCTTC CCAGCCCTTC CTGGCTGACT TCCATGGCTT CTCCTACCTG
    GAGCTGAAAG GGTTGCATAC CTTTGAGCGG GACCTGGGGG AGAAGCTGGC ACTGGAGGTG
    GTGTTCCTGG CCCGTGGGCC CAGTGGTTTG CTCCTCTACA ATGGGCAGAA AACAGACGGC
    AAGGGGGACT TTGTGTCACT GGCGTTGCAT GACCGCTTCT TGGAATTCCG CTATGACCTG
    GGCAAGGGAG CAGCGGTCAT CAGGAGCAAG GAGCCGGTGG CCCTGGACAC CTGGACCAGG
    GTCTCACTAG AGCGCAATGG CCGCAAGGGT GCCATGCGTG TCAGTGATGG GCCTCGCGTG
    CTGGGAGAGT CCCCGAAATC CCGCAAGGTG CCACACACTG TCCTCAACCT GAAGGAGCCA
    CTCTATGTTG GAGGGGCCCC TGACTTCAGC AAACTGGCCC GGGCAGCTGC GGTATTCTCT
    GGCTTCGATG GCGCCATCCA ACTGGTCTCC TTGAAAGGCC ACCAGCTGCT GTCCCAGGAG
    CACGTGGTGC GGTCAGTGGA TGTCTCATCT TTTGCCCACC ACCCTTGTAC TCAGGCCTCG
    GGCCACCCAT GCCTCAATGG GGCCTCCTGC CTCCCTCGAG AGGCCTCCTA TGAGTGCCTG
    TGCCCCGGGG GCTTCTCAGG GCTTCACTGT GAGAAGGGGC TGATTGAGAA GTCAGCAGGG
    GATGTGGACG CCCTGGCCTT TGACGGACAG ACCTACGTCG AGTACCTCAA TGCCGTGACT
    GAGAGCGAAC TGACCAATGA GATCCCAGCT CCTGATGCTC CGGATTCCGG GGCCCTTCCC
    AGTGAGAAGG CCCTGCAGAG CAACCACTTT GAGCTGAGCC TGCGCACGGA AGCCACTCAG
    GGGCTGGTGC TGTGGAGCGG CAAGGCCACG GAGCGGGCCG ACTATGTCGC ACTGGCCATC
    GTGGATGGGC GCCTGCAGCT GACCTATGAC CTGGGCTCCC AGCCCGTCGT GCTGCGCTCC
    ACCGTGCCGG TCAACACCAA CCGCTGGCTA CGAGTCAGGG CACATAGGGA ACAGAGGGAA
    GGTTCCCTTC AGGTGGGCAA TGAGGCTCCC GTGACTGGCT CTTCCCCGCT GGGTGCCACA
    CAGCTAGACA CAGACGGAAC TCTATGGCTG GGTGGCCTGG AGAAGCTGCC CATAGGCCAG
    GCCCTGCCCA AGGCCTATGG CACGGGCTTT GTGGGCTGCC TGCGGGATGT GGTGGTGGGG
    CAGCGCCCAC TGCACCTGCT GGAGGATGCT GTCACCAAGC CAGAGCTCCG ACCCTGTGCC
    GCCCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06693
    Clone ID Related Accession (Same CDS sequence) XM_005620359.2 , XM_005620359.3
    Accession Version XM_005620359.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6228bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product agrin isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726058.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005620359.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
    GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG
    GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC
    CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC
    ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA
    GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC
    CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG
    ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC
    TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC
    AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT
    CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC
    TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG
    CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC
    CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC
    CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG
    CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG
    GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC
    CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG
    TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT
    GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT
    GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG
    GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA
    TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT
    GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG
    GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG
    TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT
    GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC
    TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT
    GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC
    TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG
    GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG
    GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG
    GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT
    GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG
    CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG
    CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC
    ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT
    GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT
    GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC
    TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG
    CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT
    GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT
    GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGACT CTGTTCTCCT TGCAGAGACT
    GTTGCTCCTG GGGCCCGCCC AACATCTGTA CCCAGGGCCA CCTCAGGGCT CAGCACGAGC
    AAGGCGCTGC CACCCGCCCC CAGCACCCTC CCCCTGGCTC CCAGCAGCAC CCCTCATAGC
    CGGCCCACGC CTCGACCCTC ACCAGGATCT CGGACCACTG CCAGCATTCC CAGGACTGTC
    ACGAGGCCTG TGCTGACCAT GCCCCCCACA GCCCCCGCCA CGGCAGCCAA CCTCGCCACA
    TCTGCTTTTG GCGAGTCTGG CAGTGCTGAC GGGAGCGGCG ATGAGGGGCT GAGTGGTGAC
    CTGGAGGCCA GTGGGACTGG CTCAGGGGGA CTCGAGCCCC CTGAAGGTGG CAGTGCTGTG
    ACCCCTGAGC CACCCATGGA GAGGGCTTCT TGCTACAACT CGCCTTTGGG CTGCTGCTCG
    GATGGCAAGA CGCCCTCGCT GGACGCAGAG GGTTCCAACT GTCCTGCCAC CAAGGCGTTC
    CAGGGTGTGT TGGAGCTTGA GGGCGTGGAG GGGCAAGAGC TGTTCTACAC GCCGGAGATG
    GCTGACCCCA AGTCAGAGCT TTTTGGGGAG ACTGCTAGGA GCATCGAGAG CACCCTGGAT
    GACCTCTTCC GGAACTCAGA GGTTAAGAAG GACTTCCGAA GCGTCCGGCT GAGGGACTTG
    GGGCCTGGCA GCTCAGTCCG AGCCATTGTG GATGTGCACT TTGACCCTGC CACAACCTTT
    AGGGCGCCTG ATGTGGGCCA AGCCCTGCTC CGGCAGATAC AGGCATCCCG GCGCCGGTCC
    CTGGGCGTAC GGCGGCCACT GCAGGAGCAC GTGCGCTTTA TGGACTTTGA CTGGTTTCCT
    GCGTTCTTCA CGGGAGCCAC CACAGGAGCA ACACCCGCCG CAGCCACGGC CAGAGCCACC
    ACGGTGGCCC ACCTGCCACC TGCTGGGACC CAGTGGGCCC TCTACCCCAG TCACACAAGC
    CGGCCTGTGG GCAGGACCAC AGCTCCACCC ACCACACGCC AGCCTCCCAC CACTGCTCCC
    CGTCGCGTGC CCGGACGCCG GCCCTTGCCC CCAGGCACCC AGCAGCCCCC GAAGCCCTGC
    GACTCCCAGC CCTGCCTCCA TGGGGGAACC TGCCAGGACC AGGACTCGGG TGGAGGCTTC
    ACCTGCAGCT GCCCAGTGGG CAGGAGGGGT GCCGTCTGTG AGAAGGCCCT GTACCTCTCG
    GTGCCAGCTT TCAATGGCCG CTCCTTCCTG GCCTTCCCCA CCCTCCGTGC CTACCACACG
    CTGCGCCTAG CACTTGAGTT CCGGGCACTA GAGCCACAGG GCCTGCTGCT CTATAATGGC
    AATGCCCAGG GCAAGGACTT CCTGGCATTG ACGCTGCTGG GGGGCCGTGT GCAGCTCAGG
    TTTGACACAG GTTCCGGGCC TGCAGTACTG ACCAGCTCAG TGCCTATAGA GCCTGGCCGC
    TGGCATCGCT TGGAGCTGTC TCGGCACTGG CGCCGGGGAA CACTTTCAGT GGATGGTGAG
    CCCCTGGTGC TGGGCCACAG CCCCAGCGGT ACAGATGGTC TCAACCTGGA CACAGACCTT
    TTTGTGGGCG GCATCCCTGA GGATCAGGCT TCTGTGGTAC TCGAAAGGAC GTCCATCAGC
    GTTGGCCTCA AGGGCTGCAT CCGCCTGCTG GATGTCAATA ATCAGCGTTT AGAGCTCAGC
    AGCTGGCCAG GGGCTGCAGC CCGAAGCTCC GGGGTGGGCG AGTGTGGAGA CCACCCCTGC
    CTGCCCAACC CCTGCCTCGA CGGGGCTCCG TGCCAGGCCC TGGAGGCTGG CATGTTCCAC
    TGTCAGTGCC CACCTGGCCG CTTTGGTCCA ACCTGTAGTG GTGACAAGAA CCCCTGTGAG
    CCGAACCCCT GCCATGGGGC AGCCCCCTGC CGTGTGCTGC CTGATGGGGA GGCCAAGTGT
    GAATGCCCCC GAGGACGAGG GGGTGCCATC TGCCAGACAG TCTCGGAGCG GGATTCTTCC
    CAGCCCTTCC TGGCTGACTT CCATGGCTTC TCCTACCTGG AGCTGAAAGG GTTGCATACC
    TTTGAGCGGG ACCTGGGGGA GAAGCTGGCA CTGGAGGTGG TGTTCCTGGC CCGTGGGCCC
    AGTGGTTTGC TCCTCTACAA TGGGCAGAAA ACAGACGGCA AGGGGGACTT TGTGTCACTG
    GCGTTGCATG ACCGCTTCTT GGAATTCCGC TATGACCTGG GCAAGGGAGC AGCGGTCATC
    AGGAGCAAGG AGCCGGTGGC CCTGGACACC TGGACCAGGG TCTCACTAGA GCGCAATGGC
    CGCAAGGGTG CCATGCGTGT CAGTGATGGG CCTCGCGTGC TGGGAGAGTC CCCGAAATCC
    CGCAAGGTGC CACACACTGT CCTCAACCTG AAGGAGCCAC TCTATGTTGG AGGGGCCCCT
    GACTTCAGCA AACTGGCCCG GGCAGCTGCG GTATTCTCTG GCTTCGATGG CGCCATCCAA
    CTGGTCTCCT TGAAAGGCCA CCAGCTGCTG TCCCAGGAGC ACGTGGTGCG GTCAGTGGAT
    GTCTCATCTT TTGCCCACCA CCCTTGTACT CAGGCCTCGG GCCACCCATG CCTCAATGGG
    GCCTCCTGCC TCCCTCGAGA GGCCTCCTAT GAGTGCCTGT GCCCCGGGGG CTTCTCAGGG
    CTTCACTGTG AGAAGGGGCT GATTGAGAAG TCAGCAGGGG ATGTGGACGC CCTGGCCTTT
    GACGGACAGA CCTACGTCGA GTACCTCAAT GCCGTGACTG AGAGCGAACT GACCAATGAG
    ATCCCAGCTC CTGATGCTCC GGATTCCGGG GCCCTTCCCA GTGAGAAGGC CCTGCAGAGC
    AACCACTTTG AGCTGAGCCT GCGCACGGAA GCCACTCAGG GGCTGGTGCT GTGGAGCGGC
    AAGGCCACGG AGCGGGCCGA CTATGTCGCA CTGGCCATCG TGGATGGGCG CCTGCAGCTG
    ACCTATGACC TGGGCTCCCA GCCCGTCGTG CTGCGCTCCA CCGTGCCGGT CAACACCAAC
    CGCTGGCTAC GAGTCAGGGC ACATAGGGAA CAGAGGGAAG GTTCCCTTCA GGTGGGCAAT
    GAGGCTCCCG TGACTGGCTC TTCCCCGCTG GGTGCCACAC AGCTAGACAC AGACGGAACT
    CTATGGCTGG GTGGCCTGGA GAAGCTGCCC ATAGGCCAGG CCCTGCCCAA GGCCTATGGC
    ACGGGCTTTG TGGGCTGCCT GCGGGATGTG GTGGTGGGGC AGCGCCCACT GCACCTGCTG
    GAGGATGCTG TCACCAAGCC AGAGCTCCGA CCCTGTGCCG CCCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005620416.1
    CDS133..6360
    Translation

    Target ORF information:

    RefSeq Version XM_005620359.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris agrin (AGRN), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005620359.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
    GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG
    GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC
    CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC
    ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA
    GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC
    CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG
    ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC
    TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC
    AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT
    CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC
    TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG
    CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC
    CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC
    CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG
    CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG
    GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC
    CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG
    TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT
    GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT
    GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG
    GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA
    TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT
    GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG
    GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG
    TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT
    GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC
    TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT
    GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC
    TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG
    GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG
    GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG
    GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT
    GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG
    CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG
    CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC
    ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT
    GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT
    GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC
    TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG
    CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT
    GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT
    GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGACT CTGTTCTCCT TGCAGAGACT
    GTTGCTCCTG GGGCCCGCCC AACATCTGTA CCCAGGGCCA CCTCAGGGCT CAGCACGAGC
    AAGGCGCTGC CACCCGCCCC CAGCACCCTC CCCCTGGCTC CCAGCAGCAC CCCTCATAGC
    CGGCCCACGC CTCGACCCTC ACCAGGATCT CGGACCACTG CCAGCATTCC CAGGACTGTC
    ACGAGGCCTG TGCTGACCAT GCCCCCCACA GCCCCCGCCA CGGCAGCCAA CCTCGCCACA
    TCTGCTTTTG GCGAGTCTGG CAGTGCTGAC GGGAGCGGCG ATGAGGGGCT GAGTGGTGAC
    CTGGAGGCCA GTGGGACTGG CTCAGGGGGA CTCGAGCCCC CTGAAGGTGG CAGTGCTGTG
    ACCCCTGAGC CACCCATGGA GAGGGCTTCT TGCTACAACT CGCCTTTGGG CTGCTGCTCG
    GATGGCAAGA CGCCCTCGCT GGACGCAGAG GGTTCCAACT GTCCTGCCAC CAAGGCGTTC
    CAGGGTGTGT TGGAGCTTGA GGGCGTGGAG GGGCAAGAGC TGTTCTACAC GCCGGAGATG
    GCTGACCCCA AGTCAGAGCT TTTTGGGGAG ACTGCTAGGA GCATCGAGAG CACCCTGGAT
    GACCTCTTCC GGAACTCAGA GGTTAAGAAG GACTTCCGAA GCGTCCGGCT GAGGGACTTG
    GGGCCTGGCA GCTCAGTCCG AGCCATTGTG GATGTGCACT TTGACCCTGC CACAACCTTT
    AGGGCGCCTG ATGTGGGCCA AGCCCTGCTC CGGCAGATAC AGGCATCCCG GCGCCGGTCC
    CTGGGCGTAC GGCGGCCACT GCAGGAGCAC GTGCGCTTTA TGGACTTTGA CTGGTTTCCT
    GCGTTCTTCA CGGGAGCCAC CACAGGAGCA ACACCCGCCG CAGCCACGGC CAGAGCCACC
    ACGGTGGCCC ACCTGCCACC TGCTGGGACC CAGTGGGCCC TCTACCCCAG TCACACAAGC
    CGGCCTGTGG GCAGGACCAC AGCTCCACCC ACCACACGCC AGCCTCCCAC CACTGCTCCC
    CGTCGCGTGC CCGGACGCCG GCCCTTGCCC CCAGGCACCC AGCAGCCCCC GAAGCCCTGC
    GACTCCCAGC CCTGCCTCCA TGGGGGAACC TGCCAGGACC AGGACTCGGG TGGAGGCTTC
    ACCTGCAGCT GCCCAGTGGG CAGGAGGGGT GCCGTCTGTG AGAAGGCCCT GTACCTCTCG
    GTGCCAGCTT TCAATGGCCG CTCCTTCCTG GCCTTCCCCA CCCTCCGTGC CTACCACACG
    CTGCGCCTAG CACTTGAGTT CCGGGCACTA GAGCCACAGG GCCTGCTGCT CTATAATGGC
    AATGCCCAGG GCAAGGACTT CCTGGCATTG ACGCTGCTGG GGGGCCGTGT GCAGCTCAGG
    TTTGACACAG GTTCCGGGCC TGCAGTACTG ACCAGCTCAG TGCCTATAGA GCCTGGCCGC
    TGGCATCGCT TGGAGCTGTC TCGGCACTGG CGCCGGGGAA CACTTTCAGT GGATGGTGAG
    CCCCTGGTGC TGGGCCACAG CCCCAGCGGT ACAGATGGTC TCAACCTGGA CACAGACCTT
    TTTGTGGGCG GCATCCCTGA GGATCAGGCT TCTGTGGTAC TCGAAAGGAC GTCCATCAGC
    GTTGGCCTCA AGGGCTGCAT CCGCCTGCTG GATGTCAATA ATCAGCGTTT AGAGCTCAGC
    AGCTGGCCAG GGGCTGCAGC CCGAAGCTCC GGGGTGGGCG AGTGTGGAGA CCACCCCTGC
    CTGCCCAACC CCTGCCTCGA CGGGGCTCCG TGCCAGGCCC TGGAGGCTGG CATGTTCCAC
    TGTCAGTGCC CACCTGGCCG CTTTGGTCCA ACCTGTAGTG GTGACAAGAA CCCCTGTGAG
    CCGAACCCCT GCCATGGGGC AGCCCCCTGC CGTGTGCTGC CTGATGGGGA GGCCAAGTGT
    GAATGCCCCC GAGGACGAGG GGGTGCCATC TGCCAGACAG TCTCGGAGCG GGATTCTTCC
    CAGCCCTTCC TGGCTGACTT CCATGGCTTC TCCTACCTGG AGCTGAAAGG GTTGCATACC
    TTTGAGCGGG ACCTGGGGGA GAAGCTGGCA CTGGAGGTGG TGTTCCTGGC CCGTGGGCCC
    AGTGGTTTGC TCCTCTACAA TGGGCAGAAA ACAGACGGCA AGGGGGACTT TGTGTCACTG
    GCGTTGCATG ACCGCTTCTT GGAATTCCGC TATGACCTGG GCAAGGGAGC AGCGGTCATC
    AGGAGCAAGG AGCCGGTGGC CCTGGACACC TGGACCAGGG TCTCACTAGA GCGCAATGGC
    CGCAAGGGTG CCATGCGTGT CAGTGATGGG CCTCGCGTGC TGGGAGAGTC CCCGAAATCC
    CGCAAGGTGC CACACACTGT CCTCAACCTG AAGGAGCCAC TCTATGTTGG AGGGGCCCCT
    GACTTCAGCA AACTGGCCCG GGCAGCTGCG GTATTCTCTG GCTTCGATGG CGCCATCCAA
    CTGGTCTCCT TGAAAGGCCA CCAGCTGCTG TCCCAGGAGC ACGTGGTGCG GTCAGTGGAT
    GTCTCATCTT TTGCCCACCA CCCTTGTACT CAGGCCTCGG GCCACCCATG CCTCAATGGG
    GCCTCCTGCC TCCCTCGAGA GGCCTCCTAT GAGTGCCTGT GCCCCGGGGG CTTCTCAGGG
    CTTCACTGTG AGAAGGGGCT GATTGAGAAG TCAGCAGGGG ATGTGGACGC CCTGGCCTTT
    GACGGACAGA CCTACGTCGA GTACCTCAAT GCCGTGACTG AGAGCGAACT GACCAATGAG
    ATCCCAGCTC CTGATGCTCC GGATTCCGGG GCCCTTCCCA GTGAGAAGGC CCTGCAGAGC
    AACCACTTTG AGCTGAGCCT GCGCACGGAA GCCACTCAGG GGCTGGTGCT GTGGAGCGGC
    AAGGCCACGG AGCGGGCCGA CTATGTCGCA CTGGCCATCG TGGATGGGCG CCTGCAGCTG
    ACCTATGACC TGGGCTCCCA GCCCGTCGTG CTGCGCTCCA CCGTGCCGGT CAACACCAAC
    CGCTGGCTAC GAGTCAGGGC ACATAGGGAA CAGAGGGAAG GTTCCCTTCA GGTGGGCAAT
    GAGGCTCCCG TGACTGGCTC TTCCCCGCTG GGTGCCACAC AGCTAGACAC AGACGGAACT
    CTATGGCTGG GTGGCCTGGA GAAGCTGCCC ATAGGCCAGG CCCTGCCCAA GGCCTATGGC
    ACGGGCTTTG TGGGCTGCCT GCGGGATGTG GTGGTGGGGC AGCGCCCACT GCACCTGCTG
    GAGGATGCTG TCACCAAGCC AGAGCTCCGA CCCTGTGCCG CCCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06693
    Clone ID Related Accession (Same CDS sequence) XM_005620359.2 , XM_005620359.3
    Accession Version XM_005620359.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6228bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product agrin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005620359.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
    GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG
    GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC
    CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC
    ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA
    GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC
    CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG
    ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC
    TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC
    AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT
    CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC
    TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG
    CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC
    CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC
    CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG
    CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG
    GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC
    CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG
    TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT
    GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT
    GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG
    GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA
    TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT
    GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG
    GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG
    TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT
    GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC
    TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT
    GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC
    TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG
    GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG
    GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG
    GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT
    GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG
    CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG
    CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC
    ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT
    GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT
    GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC
    TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG
    CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT
    GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT
    GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGACT CTGTTCTCCT TGCAGAGACT
    GTTGCTCCTG GGGCCCGCCC AACATCTGTA CCCAGGGCCA CCTCAGGGCT CAGCACGAGC
    AAGGCGCTGC CACCCGCCCC CAGCACCCTC CCCCTGGCTC CCAGCAGCAC CCCTCATAGC
    CGGCCCACGC CTCGACCCTC ACCAGGATCT CGGACCACTG CCAGCATTCC CAGGACTGTC
    ACGAGGCCTG TGCTGACCAT GCCCCCCACA GCCCCCGCCA CGGCAGCCAA CCTCGCCACA
    TCTGCTTTTG GCGAGTCTGG CAGTGCTGAC GGGAGCGGCG ATGAGGGGCT GAGTGGTGAC
    CTGGAGGCCA GTGGGACTGG CTCAGGGGGA CTCGAGCCCC CTGAAGGTGG CAGTGCTGTG
    ACCCCTGAGC CACCCATGGA GAGGGCTTCT TGCTACAACT CGCCTTTGGG CTGCTGCTCG
    GATGGCAAGA CGCCCTCGCT GGACGCAGAG GGTTCCAACT GTCCTGCCAC CAAGGCGTTC
    CAGGGTGTGT TGGAGCTTGA GGGCGTGGAG GGGCAAGAGC TGTTCTACAC GCCGGAGATG
    GCTGACCCCA AGTCAGAGCT TTTTGGGGAG ACTGCTAGGA GCATCGAGAG CACCCTGGAT
    GACCTCTTCC GGAACTCAGA GGTTAAGAAG GACTTCCGAA GCGTCCGGCT GAGGGACTTG
    GGGCCTGGCA GCTCAGTCCG AGCCATTGTG GATGTGCACT TTGACCCTGC CACAACCTTT
    AGGGCGCCTG ATGTGGGCCA AGCCCTGCTC CGGCAGATAC AGGCATCCCG GCGCCGGTCC
    CTGGGCGTAC GGCGGCCACT GCAGGAGCAC GTGCGCTTTA TGGACTTTGA CTGGTTTCCT
    GCGTTCTTCA CGGGAGCCAC CACAGGAGCA ACACCCGCCG CAGCCACGGC CAGAGCCACC
    ACGGTGGCCC ACCTGCCACC TGCTGGGACC CAGTGGGCCC TCTACCCCAG TCACACAAGC
    CGGCCTGTGG GCAGGACCAC AGCTCCACCC ACCACACGCC AGCCTCCCAC CACTGCTCCC
    CGTCGCGTGC CCGGACGCCG GCCCTTGCCC CCAGGCACCC AGCAGCCCCC GAAGCCCTGC
    GACTCCCAGC CCTGCCTCCA TGGGGGAACC TGCCAGGACC AGGACTCGGG TGGAGGCTTC
    ACCTGCAGCT GCCCAGTGGG CAGGAGGGGT GCCGTCTGTG AGAAGGCCCT GTACCTCTCG
    GTGCCAGCTT TCAATGGCCG CTCCTTCCTG GCCTTCCCCA CCCTCCGTGC CTACCACACG
    CTGCGCCTAG CACTTGAGTT CCGGGCACTA GAGCCACAGG GCCTGCTGCT CTATAATGGC
    AATGCCCAGG GCAAGGACTT CCTGGCATTG ACGCTGCTGG GGGGCCGTGT GCAGCTCAGG
    TTTGACACAG GTTCCGGGCC TGCAGTACTG ACCAGCTCAG TGCCTATAGA GCCTGGCCGC
    TGGCATCGCT TGGAGCTGTC TCGGCACTGG CGCCGGGGAA CACTTTCAGT GGATGGTGAG
    CCCCTGGTGC TGGGCCACAG CCCCAGCGGT ACAGATGGTC TCAACCTGGA CACAGACCTT
    TTTGTGGGCG GCATCCCTGA GGATCAGGCT TCTGTGGTAC TCGAAAGGAC GTCCATCAGC
    GTTGGCCTCA AGGGCTGCAT CCGCCTGCTG GATGTCAATA ATCAGCGTTT AGAGCTCAGC
    AGCTGGCCAG GGGCTGCAGC CCGAAGCTCC GGGGTGGGCG AGTGTGGAGA CCACCCCTGC
    CTGCCCAACC CCTGCCTCGA CGGGGCTCCG TGCCAGGCCC TGGAGGCTGG CATGTTCCAC
    TGTCAGTGCC CACCTGGCCG CTTTGGTCCA ACCTGTAGTG GTGACAAGAA CCCCTGTGAG
    CCGAACCCCT GCCATGGGGC AGCCCCCTGC CGTGTGCTGC CTGATGGGGA GGCCAAGTGT
    GAATGCCCCC GAGGACGAGG GGGTGCCATC TGCCAGACAG TCTCGGAGCG GGATTCTTCC
    CAGCCCTTCC TGGCTGACTT CCATGGCTTC TCCTACCTGG AGCTGAAAGG GTTGCATACC
    TTTGAGCGGG ACCTGGGGGA GAAGCTGGCA CTGGAGGTGG TGTTCCTGGC CCGTGGGCCC
    AGTGGTTTGC TCCTCTACAA TGGGCAGAAA ACAGACGGCA AGGGGGACTT TGTGTCACTG
    GCGTTGCATG ACCGCTTCTT GGAATTCCGC TATGACCTGG GCAAGGGAGC AGCGGTCATC
    AGGAGCAAGG AGCCGGTGGC CCTGGACACC TGGACCAGGG TCTCACTAGA GCGCAATGGC
    CGCAAGGGTG CCATGCGTGT CAGTGATGGG CCTCGCGTGC TGGGAGAGTC CCCGAAATCC
    CGCAAGGTGC CACACACTGT CCTCAACCTG AAGGAGCCAC TCTATGTTGG AGGGGCCCCT
    GACTTCAGCA AACTGGCCCG GGCAGCTGCG GTATTCTCTG GCTTCGATGG CGCCATCCAA
    CTGGTCTCCT TGAAAGGCCA CCAGCTGCTG TCCCAGGAGC ACGTGGTGCG GTCAGTGGAT
    GTCTCATCTT TTGCCCACCA CCCTTGTACT CAGGCCTCGG GCCACCCATG CCTCAATGGG
    GCCTCCTGCC TCCCTCGAGA GGCCTCCTAT GAGTGCCTGT GCCCCGGGGG CTTCTCAGGG
    CTTCACTGTG AGAAGGGGCT GATTGAGAAG TCAGCAGGGG ATGTGGACGC CCTGGCCTTT
    GACGGACAGA CCTACGTCGA GTACCTCAAT GCCGTGACTG AGAGCGAACT GACCAATGAG
    ATCCCAGCTC CTGATGCTCC GGATTCCGGG GCCCTTCCCA GTGAGAAGGC CCTGCAGAGC
    AACCACTTTG AGCTGAGCCT GCGCACGGAA GCCACTCAGG GGCTGGTGCT GTGGAGCGGC
    AAGGCCACGG AGCGGGCCGA CTATGTCGCA CTGGCCATCG TGGATGGGCG CCTGCAGCTG
    ACCTATGACC TGGGCTCCCA GCCCGTCGTG CTGCGCTCCA CCGTGCCGGT CAACACCAAC
    CGCTGGCTAC GAGTCAGGGC ACATAGGGAA CAGAGGGAAG GTTCCCTTCA GGTGGGCAAT
    GAGGCTCCCG TGACTGGCTC TTCCCCGCTG GGTGCCACAC AGCTAGACAC AGACGGAACT
    CTATGGCTGG GTGGCCTGGA GAAGCTGCCC ATAGGCCAGG CCCTGCCCAA GGCCTATGGC
    ACGGGCTTTG TGGGCTGCCT GCGGGATGTG GTGGTGGGGC AGCGCCCACT GCACCTGCTG
    GAGGATGCTG TCACCAAGCC AGAGCTCCGA CCCTGTGCCG CCCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005620416.1
    CDS81..6308
    Translation

    Target ORF information:

    RefSeq Version XM_005620359.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris agrin (AGRN), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005620359.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
    GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG
    GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC
    CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC
    ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA
    GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC
    CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG
    ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC
    TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC
    AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT
    CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC
    TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG
    CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC
    CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC
    CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG
    CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG
    GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC
    CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG
    TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT
    GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT
    GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG
    GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA
    TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT
    GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG
    GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG
    TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT
    GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC
    TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT
    GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC
    TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG
    GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG
    GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG
    GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT
    GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG
    CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG
    CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC
    ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT
    GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT
    GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC
    TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG
    CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT
    GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT
    GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGACT CTGTTCTCCT TGCAGAGACT
    GTTGCTCCTG GGGCCCGCCC AACATCTGTA CCCAGGGCCA CCTCAGGGCT CAGCACGAGC
    AAGGCGCTGC CACCCGCCCC CAGCACCCTC CCCCTGGCTC CCAGCAGCAC CCCTCATAGC
    CGGCCCACGC CTCGACCCTC ACCAGGATCT CGGACCACTG CCAGCATTCC CAGGACTGTC
    ACGAGGCCTG TGCTGACCAT GCCCCCCACA GCCCCCGCCA CGGCAGCCAA CCTCGCCACA
    TCTGCTTTTG GCGAGTCTGG CAGTGCTGAC GGGAGCGGCG ATGAGGGGCT GAGTGGTGAC
    CTGGAGGCCA GTGGGACTGG CTCAGGGGGA CTCGAGCCCC CTGAAGGTGG CAGTGCTGTG
    ACCCCTGAGC CACCCATGGA GAGGGCTTCT TGCTACAACT CGCCTTTGGG CTGCTGCTCG
    GATGGCAAGA CGCCCTCGCT GGACGCAGAG GGTTCCAACT GTCCTGCCAC CAAGGCGTTC
    CAGGGTGTGT TGGAGCTTGA GGGCGTGGAG GGGCAAGAGC TGTTCTACAC GCCGGAGATG
    GCTGACCCCA AGTCAGAGCT TTTTGGGGAG ACTGCTAGGA GCATCGAGAG CACCCTGGAT
    GACCTCTTCC GGAACTCAGA GGTTAAGAAG GACTTCCGAA GCGTCCGGCT GAGGGACTTG
    GGGCCTGGCA GCTCAGTCCG AGCCATTGTG GATGTGCACT TTGACCCTGC CACAACCTTT
    AGGGCGCCTG ATGTGGGCCA AGCCCTGCTC CGGCAGATAC AGGCATCCCG GCGCCGGTCC
    CTGGGCGTAC GGCGGCCACT GCAGGAGCAC GTGCGCTTTA TGGACTTTGA CTGGTTTCCT
    GCGTTCTTCA CGGGAGCCAC CACAGGAGCA ACACCCGCCG CAGCCACGGC CAGAGCCACC
    ACGGTGGCCC ACCTGCCACC TGCTGGGACC CAGTGGGCCC TCTACCCCAG TCACACAAGC
    CGGCCTGTGG GCAGGACCAC AGCTCCACCC ACCACACGCC AGCCTCCCAC CACTGCTCCC
    CGTCGCGTGC CCGGACGCCG GCCCTTGCCC CCAGGCACCC AGCAGCCCCC GAAGCCCTGC
    GACTCCCAGC CCTGCCTCCA TGGGGGAACC TGCCAGGACC AGGACTCGGG TGGAGGCTTC
    ACCTGCAGCT GCCCAGTGGG CAGGAGGGGT GCCGTCTGTG AGAAGGCCCT GTACCTCTCG
    GTGCCAGCTT TCAATGGCCG CTCCTTCCTG GCCTTCCCCA CCCTCCGTGC CTACCACACG
    CTGCGCCTAG CACTTGAGTT CCGGGCACTA GAGCCACAGG GCCTGCTGCT CTATAATGGC
    AATGCCCAGG GCAAGGACTT CCTGGCATTG ACGCTGCTGG GGGGCCGTGT GCAGCTCAGG
    TTTGACACAG GTTCCGGGCC TGCAGTACTG ACCAGCTCAG TGCCTATAGA GCCTGGCCGC
    TGGCATCGCT TGGAGCTGTC TCGGCACTGG CGCCGGGGAA CACTTTCAGT GGATGGTGAG
    CCCCTGGTGC TGGGCCACAG CCCCAGCGGT ACAGATGGTC TCAACCTGGA CACAGACCTT
    TTTGTGGGCG GCATCCCTGA GGATCAGGCT TCTGTGGTAC TCGAAAGGAC GTCCATCAGC
    GTTGGCCTCA AGGGCTGCAT CCGCCTGCTG GATGTCAATA ATCAGCGTTT AGAGCTCAGC
    AGCTGGCCAG GGGCTGCAGC CCGAAGCTCC GGGGTGGGCG AGTGTGGAGA CCACCCCTGC
    CTGCCCAACC CCTGCCTCGA CGGGGCTCCG TGCCAGGCCC TGGAGGCTGG CATGTTCCAC
    TGTCAGTGCC CACCTGGCCG CTTTGGTCCA ACCTGTAGTG GTGACAAGAA CCCCTGTGAG
    CCGAACCCCT GCCATGGGGC AGCCCCCTGC CGTGTGCTGC CTGATGGGGA GGCCAAGTGT
    GAATGCCCCC GAGGACGAGG GGGTGCCATC TGCCAGACAG TCTCGGAGCG GGATTCTTCC
    CAGCCCTTCC TGGCTGACTT CCATGGCTTC TCCTACCTGG AGCTGAAAGG GTTGCATACC
    TTTGAGCGGG ACCTGGGGGA GAAGCTGGCA CTGGAGGTGG TGTTCCTGGC CCGTGGGCCC
    AGTGGTTTGC TCCTCTACAA TGGGCAGAAA ACAGACGGCA AGGGGGACTT TGTGTCACTG
    GCGTTGCATG ACCGCTTCTT GGAATTCCGC TATGACCTGG GCAAGGGAGC AGCGGTCATC
    AGGAGCAAGG AGCCGGTGGC CCTGGACACC TGGACCAGGG TCTCACTAGA GCGCAATGGC
    CGCAAGGGTG CCATGCGTGT CAGTGATGGG CCTCGCGTGC TGGGAGAGTC CCCGAAATCC
    CGCAAGGTGC CACACACTGT CCTCAACCTG AAGGAGCCAC TCTATGTTGG AGGGGCCCCT
    GACTTCAGCA AACTGGCCCG GGCAGCTGCG GTATTCTCTG GCTTCGATGG CGCCATCCAA
    CTGGTCTCCT TGAAAGGCCA CCAGCTGCTG TCCCAGGAGC ACGTGGTGCG GTCAGTGGAT
    GTCTCATCTT TTGCCCACCA CCCTTGTACT CAGGCCTCGG GCCACCCATG CCTCAATGGG
    GCCTCCTGCC TCCCTCGAGA GGCCTCCTAT GAGTGCCTGT GCCCCGGGGG CTTCTCAGGG
    CTTCACTGTG AGAAGGGGCT GATTGAGAAG TCAGCAGGGG ATGTGGACGC CCTGGCCTTT
    GACGGACAGA CCTACGTCGA GTACCTCAAT GCCGTGACTG AGAGCGAACT GACCAATGAG
    ATCCCAGCTC CTGATGCTCC GGATTCCGGG GCCCTTCCCA GTGAGAAGGC CCTGCAGAGC
    AACCACTTTG AGCTGAGCCT GCGCACGGAA GCCACTCAGG GGCTGGTGCT GTGGAGCGGC
    AAGGCCACGG AGCGGGCCGA CTATGTCGCA CTGGCCATCG TGGATGGGCG CCTGCAGCTG
    ACCTATGACC TGGGCTCCCA GCCCGTCGTG CTGCGCTCCA CCGTGCCGGT CAACACCAAC
    CGCTGGCTAC GAGTCAGGGC ACATAGGGAA CAGAGGGAAG GTTCCCTTCA GGTGGGCAAT
    GAGGCTCCCG TGACTGGCTC TTCCCCGCTG GGTGCCACAC AGCTAGACAC AGACGGAACT
    CTATGGCTGG GTGGCCTGGA GAAGCTGCCC ATAGGCCAGG CCCTGCCCAA GGCCTATGGC
    ACGGGCTTTG TGGGCTGCCT GCGGGATGTG GTGGTGGGGC AGCGCCCACT GCACCTGCTG
    GAGGATGCTG TCACCAAGCC AGAGCTCCGA CCCTGTGCCG CCCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06694
    Clone ID Related Accession (Same CDS sequence) XM_005620360.3 , XM_005620360.2
    Accession Version XM_005620360.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6216bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product agrin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005620360.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
    GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG
    GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC
    CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC
    ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA
    GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC
    CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG
    ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC
    TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC
    AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT
    CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC
    TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG
    CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC
    CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC
    CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG
    CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG
    GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC
    CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG
    TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT
    GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT
    GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG
    GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA
    TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT
    GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG
    GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG
    TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT
    GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC
    TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT
    GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC
    TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG
    GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG
    GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG
    GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT
    GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG
    CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG
    CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC
    ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT
    GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT
    GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC
    TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG
    CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT
    GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT
    GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGACT CTGTTCTCCT TGCAGAGACT
    GTTGCTCCTG GGGCCCGCCC AACATCTGTA CCCAGGGCCA CCTCAGGGCT CAGCACGAGC
    AAGGCGCTGC CACCCGCCCC CAGCACCCTC CCCCTGGCTC CCAGCAGCAC CCCTCATAGC
    CGGCCCACGC CTCGACCCTC ACCAGGATCT CGGACCACTG CCAGCATTCC CAGGACTGTC
    ACGAGGCCTG TGCTGACCAT GCCCCCCACA GCCCCCGCCA CGGCAGCCAA CCTCGCCACA
    TCTGCTTTTG GCGAGTCTGG CAGTGCTGAC GGGAGCGGCG ATGAGGGGCT GAGTGGTGAC
    CTGGAGGCCA GTGGGACTGG CTCAGGGGGA CTCGAGCCCC CTGAAGGTGG CAGTGCTGTG
    ACCCCTGAGC CACCCATGGA GAGGGCTTCT TGCTACAACT CGCCTTTGGG CTGCTGCTCG
    GATGGCAAGA CGCCCTCGCT GGACGCAGAG GGTTCCAACT GTCCTGCCAC CAAGGCGTTC
    CAGGGTGTGT TGGAGCTTGA GGGCGTGGAG GGGCAAGAGC TGTTCTACAC GCCGGAGATG
    GCTGACCCCA AGTCAGAGCT TTTTGGGGAG ACTGCTAGGA GCATCGAGAG CACCCTGGAT
    GACCTCTTCC GGAACTCAGA GGTTAAGAAG GACTTCCGAA GCGTCCGGCT GAGGGACTTG
    GGGCCTGGCA GCTCAGTCCG AGCCATTGTG GATGTGCACT TTGACCCTGC CACAACCTTT
    AGGGCGCCTG ATGTGGGCCA AGCCCTGCTC CGGCAGATAC AGGCATCCCG GCGCCGGTCC
    CTGGGCGTAC GGCGGCCACT GCAGGAGCAC GTGCGCTTTA TGGACTTTGA CTGGTTTCCT
    GCGTTCTTCA CGGGAGCCAC CACAGGAGCA ACACCCGCCG CAGCCACGGC CAGAGCCACC
    ACGGTGGCCC ACCTGCCACC TGCTGGGACC CAGTGGGCCC TCTACCCCAG TCACACAAGC
    CGGCCTGTGG GCAGGACCAC AGCTCCACCC ACCACACGCC AGCCTCCCAC CACTGCTCCC
    CGTCGCGTGC CCGGACGCCG GCCCTTGCCC CCAGGCACCC AGCAGCCCCC GAAGCCCTGC
    GACTCCCAGC CCTGCCTCCA TGGGGGAACC TGCCAGGACC AGGACTCGGG TGGAGGCTTC
    ACCTGCAGCT GCCCAGTGGG CAGGAGGGGT GCCGTCTGTG AGAAGGCCCT GTACCTCTCG
    GTGCCAGCTT TCAATGGCCG CTCCTTCCTG GCCTTCCCCA CCCTCCGTGC CTACCACACG
    CTGCGCCTAG CACTTGAGTT CCGGGCACTA GAGCCACAGG GCCTGCTGCT CTATAATGGC
    AATGCCCAGG GCAAGGACTT CCTGGCATTG ACGCTGCTGG GGGGCCGTGT GCAGCTCAGG
    TTTGACACAG GTTCCGGGCC TGCAGTACTG ACCAGCTCAG TGCCTATAGA GCCTGGCCGC
    TGGCATCGCT TGGAGCTGTC TCGGCACTGG CGCCGGGGAA CACTTTCAGT GGATGGTGAG
    CCCCTGGTGC TGGGCCACAG CCCCAGCGGT ACAGATGGTC TCAACCTGGA CACAGACCTT
    TTTGTGGGCG GCATCCCTGA GGATCAGGCT TCTGTGGTAC TCGAAAGGAC GTCCATCAGC
    GTTGGCCTCA AGGGCTGCAT CCGCCTGCTG GATGTCAATA ATCAGCGTTT AGAGCTCAGC
    AGCTGGCCAG GGGCTGCAGC CCGAAGCTCC GGGGTGGGCG AGTGTGGAGA CCACCCCTGC
    CTGCCCAACC CCTGCCTCGA CGGGGCTCCG TGCCAGGCCC TGGAGGCTGG CATGTTCCAC
    TGTCAGTGCC CACCTGGCCG CTTTGGTCCA ACCTGTAGTG GTGACAAGAA CCCCTGTGAG
    CCGAACCCCT GCCATGGGGC AGCCCCCTGC CGTGTGCTGC CTGATGGGGA GGCCAAGTGT
    GAATGCCCCC GAGGACGAGG GGGTGCCATC TGCCAGACAG TCTCGGAGCG GGATTCTTCC
    CAGCCCTTCC TGGCTGACTT CCATGGCTTC TCCTACCTGG AGCTGAAAGG GTTGCATACC
    TTTGAGCGGG ACCTGGGGGA GAAGCTGGCA CTGGAGGTGG TGTTCCTGGC CCGTGGGCCC
    AGTGGTTTGC TCCTCTACAA TGGGCAGAAA ACAGACGGCA AGGGGGACTT TGTGTCACTG
    GCGTTGCATG ACCGCTTCTT GGAATTCCGC TATGACCTGG GCAAGGGAGC AGCGGTCATC
    AGGAGCAAGG AGCCGGTGGC CCTGGACACC TGGACCAGGG TCTCACTAGA GCGCAATGGC
    CGCAAGGGTG CCATGCGTGT CAGTGATGGG CCTCGCGTGC TGGGAGAGTC CCCGGTGCCA
    CACACTGTCC TCAACCTGAA GGAGCCACTC TATGTTGGAG GGGCCCCTGA CTTCAGCAAA
    CTGGCCCGGG CAGCTGCGGT ATTCTCTGGC TTCGATGGCG CCATCCAACT GGTCTCCTTG
    AAAGGCCACC AGCTGCTGTC CCAGGAGCAC GTGGTGCGGT CAGTGGATGT CTCATCTTTT
    GCCCACCACC CTTGTACTCA GGCCTCGGGC CACCCATGCC TCAATGGGGC CTCCTGCCTC
    CCTCGAGAGG CCTCCTATGA GTGCCTGTGC CCCGGGGGCT TCTCAGGGCT TCACTGTGAG
    AAGGGGCTGA TTGAGAAGTC AGCAGGGGAT GTGGACGCCC TGGCCTTTGA CGGACAGACC
    TACGTCGAGT ACCTCAATGC CGTGACTGAG AGCGAACTGA CCAATGAGAT CCCAGCTCCT
    GATGCTCCGG ATTCCGGGGC CCTTCCCAGT GAGAAGGCCC TGCAGAGCAA CCACTTTGAG
    CTGAGCCTGC GCACGGAAGC CACTCAGGGG CTGGTGCTGT GGAGCGGCAA GGCCACGGAG
    CGGGCCGACT ATGTCGCACT GGCCATCGTG GATGGGCGCC TGCAGCTGAC CTATGACCTG
    GGCTCCCAGC CCGTCGTGCT GCGCTCCACC GTGCCGGTCA ACACCAACCG CTGGCTACGA
    GTCAGGGCAC ATAGGGAACA GAGGGAAGGT TCCCTTCAGG TGGGCAATGA GGCTCCCGTG
    ACTGGCTCTT CCCCGCTGGG TGCCACACAG CTAGACACAG ACGGAACTCT ATGGCTGGGT
    GGCCTGGAGA AGCTGCCCAT AGGCCAGGCC CTGCCCAAGG CCTATGGCAC GGGCTTTGTG
    GGCTGCCTGC GGGATGTGGT GGTGGGGCAG CGCCCACTGC ACCTGCTGGA GGATGCTGTC
    ACCAAGCCAG AGCTCCGACC CTGTGCCGCC CCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005620417.1
    CDS81..6296
    Translation

    Target ORF information:

    RefSeq Version XM_005620360.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris agrin (AGRN), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005620360.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
    GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG
    GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC
    CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC
    ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA
    GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC
    CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG
    ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC
    TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC
    AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT
    CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC
    TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG
    CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC
    CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC
    CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG
    CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG
    GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC
    CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG
    TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT
    GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT
    GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG
    GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA
    TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT
    GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG
    GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG
    TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT
    GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC
    TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT
    GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC
    TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG
    GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG
    GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG
    GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT
    GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG
    CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG
    CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC
    ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT
    GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT
    GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC
    TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG
    CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT
    GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT
    GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGACT CTGTTCTCCT TGCAGAGACT
    GTTGCTCCTG GGGCCCGCCC AACATCTGTA CCCAGGGCCA CCTCAGGGCT CAGCACGAGC
    AAGGCGCTGC CACCCGCCCC CAGCACCCTC CCCCTGGCTC CCAGCAGCAC CCCTCATAGC
    CGGCCCACGC CTCGACCCTC ACCAGGATCT CGGACCACTG CCAGCATTCC CAGGACTGTC
    ACGAGGCCTG TGCTGACCAT GCCCCCCACA GCCCCCGCCA CGGCAGCCAA CCTCGCCACA
    TCTGCTTTTG GCGAGTCTGG CAGTGCTGAC GGGAGCGGCG ATGAGGGGCT GAGTGGTGAC
    CTGGAGGCCA GTGGGACTGG CTCAGGGGGA CTCGAGCCCC CTGAAGGTGG CAGTGCTGTG
    ACCCCTGAGC CACCCATGGA GAGGGCTTCT TGCTACAACT CGCCTTTGGG CTGCTGCTCG
    GATGGCAAGA CGCCCTCGCT GGACGCAGAG GGTTCCAACT GTCCTGCCAC CAAGGCGTTC
    CAGGGTGTGT TGGAGCTTGA GGGCGTGGAG GGGCAAGAGC TGTTCTACAC GCCGGAGATG
    GCTGACCCCA AGTCAGAGCT TTTTGGGGAG ACTGCTAGGA GCATCGAGAG CACCCTGGAT
    GACCTCTTCC GGAACTCAGA GGTTAAGAAG GACTTCCGAA GCGTCCGGCT GAGGGACTTG
    GGGCCTGGCA GCTCAGTCCG AGCCATTGTG GATGTGCACT TTGACCCTGC CACAACCTTT
    AGGGCGCCTG ATGTGGGCCA AGCCCTGCTC CGGCAGATAC AGGCATCCCG GCGCCGGTCC
    CTGGGCGTAC GGCGGCCACT GCAGGAGCAC GTGCGCTTTA TGGACTTTGA CTGGTTTCCT
    GCGTTCTTCA CGGGAGCCAC CACAGGAGCA ACACCCGCCG CAGCCACGGC CAGAGCCACC
    ACGGTGGCCC ACCTGCCACC TGCTGGGACC CAGTGGGCCC TCTACCCCAG TCACACAAGC
    CGGCCTGTGG GCAGGACCAC AGCTCCACCC ACCACACGCC AGCCTCCCAC CACTGCTCCC
    CGTCGCGTGC CCGGACGCCG GCCCTTGCCC CCAGGCACCC AGCAGCCCCC GAAGCCCTGC
    GACTCCCAGC CCTGCCTCCA TGGGGGAACC TGCCAGGACC AGGACTCGGG TGGAGGCTTC
    ACCTGCAGCT GCCCAGTGGG CAGGAGGGGT GCCGTCTGTG AGAAGGCCCT GTACCTCTCG
    GTGCCAGCTT TCAATGGCCG CTCCTTCCTG GCCTTCCCCA CCCTCCGTGC CTACCACACG
    CTGCGCCTAG CACTTGAGTT CCGGGCACTA GAGCCACAGG GCCTGCTGCT CTATAATGGC
    AATGCCCAGG GCAAGGACTT CCTGGCATTG ACGCTGCTGG GGGGCCGTGT GCAGCTCAGG
    TTTGACACAG GTTCCGGGCC TGCAGTACTG ACCAGCTCAG TGCCTATAGA GCCTGGCCGC
    TGGCATCGCT TGGAGCTGTC TCGGCACTGG CGCCGGGGAA CACTTTCAGT GGATGGTGAG
    CCCCTGGTGC TGGGCCACAG CCCCAGCGGT ACAGATGGTC TCAACCTGGA CACAGACCTT
    TTTGTGGGCG GCATCCCTGA GGATCAGGCT TCTGTGGTAC TCGAAAGGAC GTCCATCAGC
    GTTGGCCTCA AGGGCTGCAT CCGCCTGCTG GATGTCAATA ATCAGCGTTT AGAGCTCAGC
    AGCTGGCCAG GGGCTGCAGC CCGAAGCTCC GGGGTGGGCG AGTGTGGAGA CCACCCCTGC
    CTGCCCAACC CCTGCCTCGA CGGGGCTCCG TGCCAGGCCC TGGAGGCTGG CATGTTCCAC
    TGTCAGTGCC CACCTGGCCG CTTTGGTCCA ACCTGTAGTG GTGACAAGAA CCCCTGTGAG
    CCGAACCCCT GCCATGGGGC AGCCCCCTGC CGTGTGCTGC CTGATGGGGA GGCCAAGTGT
    GAATGCCCCC GAGGACGAGG GGGTGCCATC TGCCAGACAG TCTCGGAGCG GGATTCTTCC
    CAGCCCTTCC TGGCTGACTT CCATGGCTTC TCCTACCTGG AGCTGAAAGG GTTGCATACC
    TTTGAGCGGG ACCTGGGGGA GAAGCTGGCA CTGGAGGTGG TGTTCCTGGC CCGTGGGCCC
    AGTGGTTTGC TCCTCTACAA TGGGCAGAAA ACAGACGGCA AGGGGGACTT TGTGTCACTG
    GCGTTGCATG ACCGCTTCTT GGAATTCCGC TATGACCTGG GCAAGGGAGC AGCGGTCATC
    AGGAGCAAGG AGCCGGTGGC CCTGGACACC TGGACCAGGG TCTCACTAGA GCGCAATGGC
    CGCAAGGGTG CCATGCGTGT CAGTGATGGG CCTCGCGTGC TGGGAGAGTC CCCGGTGCCA
    CACACTGTCC TCAACCTGAA GGAGCCACTC TATGTTGGAG GGGCCCCTGA CTTCAGCAAA
    CTGGCCCGGG CAGCTGCGGT ATTCTCTGGC TTCGATGGCG CCATCCAACT GGTCTCCTTG
    AAAGGCCACC AGCTGCTGTC CCAGGAGCAC GTGGTGCGGT CAGTGGATGT CTCATCTTTT
    GCCCACCACC CTTGTACTCA GGCCTCGGGC CACCCATGCC TCAATGGGGC CTCCTGCCTC
    CCTCGAGAGG CCTCCTATGA GTGCCTGTGC CCCGGGGGCT TCTCAGGGCT TCACTGTGAG
    AAGGGGCTGA TTGAGAAGTC AGCAGGGGAT GTGGACGCCC TGGCCTTTGA CGGACAGACC
    TACGTCGAGT ACCTCAATGC CGTGACTGAG AGCGAACTGA CCAATGAGAT CCCAGCTCCT
    GATGCTCCGG ATTCCGGGGC CCTTCCCAGT GAGAAGGCCC TGCAGAGCAA CCACTTTGAG
    CTGAGCCTGC GCACGGAAGC CACTCAGGGG CTGGTGCTGT GGAGCGGCAA GGCCACGGAG
    CGGGCCGACT ATGTCGCACT GGCCATCGTG GATGGGCGCC TGCAGCTGAC CTATGACCTG
    GGCTCCCAGC CCGTCGTGCT GCGCTCCACC GTGCCGGTCA ACACCAACCG CTGGCTACGA
    GTCAGGGCAC ATAGGGAACA GAGGGAAGGT TCCCTTCAGG TGGGCAATGA GGCTCCCGTG
    ACTGGCTCTT CCCCGCTGGG TGCCACACAG CTAGACACAG ACGGAACTCT ATGGCTGGGT
    GGCCTGGAGA AGCTGCCCAT AGGCCAGGCC CTGCCCAAGG CCTATGGCAC GGGCTTTGTG
    GGCTGCCTGC GGGATGTGGT GGTGGGGCAG CGCCCACTGC ACCTGCTGGA GGATGCTGTC
    ACCAAGCCAG AGCTCCGACC CTGTGCCGCC CCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06694
    Clone ID Related Accession (Same CDS sequence) XM_005620360.3 , XM_005620360.2
    Accession Version XM_005620360.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6216bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product agrin isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726058.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005620360.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
    GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG
    GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC
    CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC
    ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA
    GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC
    CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG
    ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC
    TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC
    AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT
    CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC
    TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG
    CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC
    CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC
    CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG
    CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG
    GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC
    CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG
    TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT
    GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT
    GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG
    GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA
    TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT
    GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG
    GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG
    TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT
    GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC
    TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT
    GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC
    TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG
    GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG
    GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG
    GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT
    GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG
    CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG
    CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC
    ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT
    GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT
    GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC
    TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG
    CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT
    GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT
    GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGACT CTGTTCTCCT TGCAGAGACT
    GTTGCTCCTG GGGCCCGCCC AACATCTGTA CCCAGGGCCA CCTCAGGGCT CAGCACGAGC
    AAGGCGCTGC CACCCGCCCC CAGCACCCTC CCCCTGGCTC CCAGCAGCAC CCCTCATAGC
    CGGCCCACGC CTCGACCCTC ACCAGGATCT CGGACCACTG CCAGCATTCC CAGGACTGTC
    ACGAGGCCTG TGCTGACCAT GCCCCCCACA GCCCCCGCCA CGGCAGCCAA CCTCGCCACA
    TCTGCTTTTG GCGAGTCTGG CAGTGCTGAC GGGAGCGGCG ATGAGGGGCT GAGTGGTGAC
    CTGGAGGCCA GTGGGACTGG CTCAGGGGGA CTCGAGCCCC CTGAAGGTGG CAGTGCTGTG
    ACCCCTGAGC CACCCATGGA GAGGGCTTCT TGCTACAACT CGCCTTTGGG CTGCTGCTCG
    GATGGCAAGA CGCCCTCGCT GGACGCAGAG GGTTCCAACT GTCCTGCCAC CAAGGCGTTC
    CAGGGTGTGT TGGAGCTTGA GGGCGTGGAG GGGCAAGAGC TGTTCTACAC GCCGGAGATG
    GCTGACCCCA AGTCAGAGCT TTTTGGGGAG ACTGCTAGGA GCATCGAGAG CACCCTGGAT
    GACCTCTTCC GGAACTCAGA GGTTAAGAAG GACTTCCGAA GCGTCCGGCT GAGGGACTTG
    GGGCCTGGCA GCTCAGTCCG AGCCATTGTG GATGTGCACT TTGACCCTGC CACAACCTTT
    AGGGCGCCTG ATGTGGGCCA AGCCCTGCTC CGGCAGATAC AGGCATCCCG GCGCCGGTCC
    CTGGGCGTAC GGCGGCCACT GCAGGAGCAC GTGCGCTTTA TGGACTTTGA CTGGTTTCCT
    GCGTTCTTCA CGGGAGCCAC CACAGGAGCA ACACCCGCCG CAGCCACGGC CAGAGCCACC
    ACGGTGGCCC ACCTGCCACC TGCTGGGACC CAGTGGGCCC TCTACCCCAG TCACACAAGC
    CGGCCTGTGG GCAGGACCAC AGCTCCACCC ACCACACGCC AGCCTCCCAC CACTGCTCCC
    CGTCGCGTGC CCGGACGCCG GCCCTTGCCC CCAGGCACCC AGCAGCCCCC GAAGCCCTGC
    GACTCCCAGC CCTGCCTCCA TGGGGGAACC TGCCAGGACC AGGACTCGGG TGGAGGCTTC
    ACCTGCAGCT GCCCAGTGGG CAGGAGGGGT GCCGTCTGTG AGAAGGCCCT GTACCTCTCG
    GTGCCAGCTT TCAATGGCCG CTCCTTCCTG GCCTTCCCCA CCCTCCGTGC CTACCACACG
    CTGCGCCTAG CACTTGAGTT CCGGGCACTA GAGCCACAGG GCCTGCTGCT CTATAATGGC
    AATGCCCAGG GCAAGGACTT CCTGGCATTG ACGCTGCTGG GGGGCCGTGT GCAGCTCAGG
    TTTGACACAG GTTCCGGGCC TGCAGTACTG ACCAGCTCAG TGCCTATAGA GCCTGGCCGC
    TGGCATCGCT TGGAGCTGTC TCGGCACTGG CGCCGGGGAA CACTTTCAGT GGATGGTGAG
    CCCCTGGTGC TGGGCCACAG CCCCAGCGGT ACAGATGGTC TCAACCTGGA CACAGACCTT
    TTTGTGGGCG GCATCCCTGA GGATCAGGCT TCTGTGGTAC TCGAAAGGAC GTCCATCAGC
    GTTGGCCTCA AGGGCTGCAT CCGCCTGCTG GATGTCAATA ATCAGCGTTT AGAGCTCAGC
    AGCTGGCCAG GGGCTGCAGC CCGAAGCTCC GGGGTGGGCG AGTGTGGAGA CCACCCCTGC
    CTGCCCAACC CCTGCCTCGA CGGGGCTCCG TGCCAGGCCC TGGAGGCTGG CATGTTCCAC
    TGTCAGTGCC CACCTGGCCG CTTTGGTCCA ACCTGTAGTG GTGACAAGAA CCCCTGTGAG
    CCGAACCCCT GCCATGGGGC AGCCCCCTGC CGTGTGCTGC CTGATGGGGA GGCCAAGTGT
    GAATGCCCCC GAGGACGAGG GGGTGCCATC TGCCAGACAG TCTCGGAGCG GGATTCTTCC
    CAGCCCTTCC TGGCTGACTT CCATGGCTTC TCCTACCTGG AGCTGAAAGG GTTGCATACC
    TTTGAGCGGG ACCTGGGGGA GAAGCTGGCA CTGGAGGTGG TGTTCCTGGC CCGTGGGCCC
    AGTGGTTTGC TCCTCTACAA TGGGCAGAAA ACAGACGGCA AGGGGGACTT TGTGTCACTG
    GCGTTGCATG ACCGCTTCTT GGAATTCCGC TATGACCTGG GCAAGGGAGC AGCGGTCATC
    AGGAGCAAGG AGCCGGTGGC CCTGGACACC TGGACCAGGG TCTCACTAGA GCGCAATGGC
    CGCAAGGGTG CCATGCGTGT CAGTGATGGG CCTCGCGTGC TGGGAGAGTC CCCGGTGCCA
    CACACTGTCC TCAACCTGAA GGAGCCACTC TATGTTGGAG GGGCCCCTGA CTTCAGCAAA
    CTGGCCCGGG CAGCTGCGGT ATTCTCTGGC TTCGATGGCG CCATCCAACT GGTCTCCTTG
    AAAGGCCACC AGCTGCTGTC CCAGGAGCAC GTGGTGCGGT CAGTGGATGT CTCATCTTTT
    GCCCACCACC CTTGTACTCA GGCCTCGGGC CACCCATGCC TCAATGGGGC CTCCTGCCTC
    CCTCGAGAGG CCTCCTATGA GTGCCTGTGC CCCGGGGGCT TCTCAGGGCT TCACTGTGAG
    AAGGGGCTGA TTGAGAAGTC AGCAGGGGAT GTGGACGCCC TGGCCTTTGA CGGACAGACC
    TACGTCGAGT ACCTCAATGC CGTGACTGAG AGCGAACTGA CCAATGAGAT CCCAGCTCCT
    GATGCTCCGG ATTCCGGGGC CCTTCCCAGT GAGAAGGCCC TGCAGAGCAA CCACTTTGAG
    CTGAGCCTGC GCACGGAAGC CACTCAGGGG CTGGTGCTGT GGAGCGGCAA GGCCACGGAG
    CGGGCCGACT ATGTCGCACT GGCCATCGTG GATGGGCGCC TGCAGCTGAC CTATGACCTG
    GGCTCCCAGC CCGTCGTGCT GCGCTCCACC GTGCCGGTCA ACACCAACCG CTGGCTACGA
    GTCAGGGCAC ATAGGGAACA GAGGGAAGGT TCCCTTCAGG TGGGCAATGA GGCTCCCGTG
    ACTGGCTCTT CCCCGCTGGG TGCCACACAG CTAGACACAG ACGGAACTCT ATGGCTGGGT
    GGCCTGGAGA AGCTGCCCAT AGGCCAGGCC CTGCCCAAGG CCTATGGCAC GGGCTTTGTG
    GGCTGCCTGC GGGATGTGGT GGTGGGGCAG CGCCCACTGC ACCTGCTGGA GGATGCTGTC
    ACCAAGCCAG AGCTCCGACC CTGTGCCGCC CCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005620417.1
    CDS133..6348
    Translation

    Target ORF information:

    RefSeq Version XM_005620360.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris agrin (AGRN), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005620360.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
    GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG
    GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC
    CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC
    ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA
    GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC
    CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG
    ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC
    TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC
    AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT
    CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC
    TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG
    CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC
    CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC
    CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG
    CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG
    GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC
    CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG
    TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT
    GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT
    GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG
    GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA
    TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT
    GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG
    GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG
    TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT
    GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC
    TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT
    GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC
    TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG
    GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG
    GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG
    GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT
    GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG
    CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG
    CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC
    ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT
    GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT
    GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC
    TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG
    CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT
    GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT
    GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGACT CTGTTCTCCT TGCAGAGACT
    GTTGCTCCTG GGGCCCGCCC AACATCTGTA CCCAGGGCCA CCTCAGGGCT CAGCACGAGC
    AAGGCGCTGC CACCCGCCCC CAGCACCCTC CCCCTGGCTC CCAGCAGCAC CCCTCATAGC
    CGGCCCACGC CTCGACCCTC ACCAGGATCT CGGACCACTG CCAGCATTCC CAGGACTGTC
    ACGAGGCCTG TGCTGACCAT GCCCCCCACA GCCCCCGCCA CGGCAGCCAA CCTCGCCACA
    TCTGCTTTTG GCGAGTCTGG CAGTGCTGAC GGGAGCGGCG ATGAGGGGCT GAGTGGTGAC
    CTGGAGGCCA GTGGGACTGG CTCAGGGGGA CTCGAGCCCC CTGAAGGTGG CAGTGCTGTG
    ACCCCTGAGC CACCCATGGA GAGGGCTTCT TGCTACAACT CGCCTTTGGG CTGCTGCTCG
    GATGGCAAGA CGCCCTCGCT GGACGCAGAG GGTTCCAACT GTCCTGCCAC CAAGGCGTTC
    CAGGGTGTGT TGGAGCTTGA GGGCGTGGAG GGGCAAGAGC TGTTCTACAC GCCGGAGATG
    GCTGACCCCA AGTCAGAGCT TTTTGGGGAG ACTGCTAGGA GCATCGAGAG CACCCTGGAT
    GACCTCTTCC GGAACTCAGA GGTTAAGAAG GACTTCCGAA GCGTCCGGCT GAGGGACTTG
    GGGCCTGGCA GCTCAGTCCG AGCCATTGTG GATGTGCACT TTGACCCTGC CACAACCTTT
    AGGGCGCCTG ATGTGGGCCA AGCCCTGCTC CGGCAGATAC AGGCATCCCG GCGCCGGTCC
    CTGGGCGTAC GGCGGCCACT GCAGGAGCAC GTGCGCTTTA TGGACTTTGA CTGGTTTCCT
    GCGTTCTTCA CGGGAGCCAC CACAGGAGCA ACACCCGCCG CAGCCACGGC CAGAGCCACC
    ACGGTGGCCC ACCTGCCACC TGCTGGGACC CAGTGGGCCC TCTACCCCAG TCACACAAGC
    CGGCCTGTGG GCAGGACCAC AGCTCCACCC ACCACACGCC AGCCTCCCAC CACTGCTCCC
    CGTCGCGTGC CCGGACGCCG GCCCTTGCCC CCAGGCACCC AGCAGCCCCC GAAGCCCTGC
    GACTCCCAGC CCTGCCTCCA TGGGGGAACC TGCCAGGACC AGGACTCGGG TGGAGGCTTC
    ACCTGCAGCT GCCCAGTGGG CAGGAGGGGT GCCGTCTGTG AGAAGGCCCT GTACCTCTCG
    GTGCCAGCTT TCAATGGCCG CTCCTTCCTG GCCTTCCCCA CCCTCCGTGC CTACCACACG
    CTGCGCCTAG CACTTGAGTT CCGGGCACTA GAGCCACAGG GCCTGCTGCT CTATAATGGC
    AATGCCCAGG GCAAGGACTT CCTGGCATTG ACGCTGCTGG GGGGCCGTGT GCAGCTCAGG
    TTTGACACAG GTTCCGGGCC TGCAGTACTG ACCAGCTCAG TGCCTATAGA GCCTGGCCGC
    TGGCATCGCT TGGAGCTGTC TCGGCACTGG CGCCGGGGAA CACTTTCAGT GGATGGTGAG
    CCCCTGGTGC TGGGCCACAG CCCCAGCGGT ACAGATGGTC TCAACCTGGA CACAGACCTT
    TTTGTGGGCG GCATCCCTGA GGATCAGGCT TCTGTGGTAC TCGAAAGGAC GTCCATCAGC
    GTTGGCCTCA AGGGCTGCAT CCGCCTGCTG GATGTCAATA ATCAGCGTTT AGAGCTCAGC
    AGCTGGCCAG GGGCTGCAGC CCGAAGCTCC GGGGTGGGCG AGTGTGGAGA CCACCCCTGC
    CTGCCCAACC CCTGCCTCGA CGGGGCTCCG TGCCAGGCCC TGGAGGCTGG CATGTTCCAC
    TGTCAGTGCC CACCTGGCCG CTTTGGTCCA ACCTGTAGTG GTGACAAGAA CCCCTGTGAG
    CCGAACCCCT GCCATGGGGC AGCCCCCTGC CGTGTGCTGC CTGATGGGGA GGCCAAGTGT
    GAATGCCCCC GAGGACGAGG GGGTGCCATC TGCCAGACAG TCTCGGAGCG GGATTCTTCC
    CAGCCCTTCC TGGCTGACTT CCATGGCTTC TCCTACCTGG AGCTGAAAGG GTTGCATACC
    TTTGAGCGGG ACCTGGGGGA GAAGCTGGCA CTGGAGGTGG TGTTCCTGGC CCGTGGGCCC
    AGTGGTTTGC TCCTCTACAA TGGGCAGAAA ACAGACGGCA AGGGGGACTT TGTGTCACTG
    GCGTTGCATG ACCGCTTCTT GGAATTCCGC TATGACCTGG GCAAGGGAGC AGCGGTCATC
    AGGAGCAAGG AGCCGGTGGC CCTGGACACC TGGACCAGGG TCTCACTAGA GCGCAATGGC
    CGCAAGGGTG CCATGCGTGT CAGTGATGGG CCTCGCGTGC TGGGAGAGTC CCCGGTGCCA
    CACACTGTCC TCAACCTGAA GGAGCCACTC TATGTTGGAG GGGCCCCTGA CTTCAGCAAA
    CTGGCCCGGG CAGCTGCGGT ATTCTCTGGC TTCGATGGCG CCATCCAACT GGTCTCCTTG
    AAAGGCCACC AGCTGCTGTC CCAGGAGCAC GTGGTGCGGT CAGTGGATGT CTCATCTTTT
    GCCCACCACC CTTGTACTCA GGCCTCGGGC CACCCATGCC TCAATGGGGC CTCCTGCCTC
    CCTCGAGAGG CCTCCTATGA GTGCCTGTGC CCCGGGGGCT TCTCAGGGCT TCACTGTGAG
    AAGGGGCTGA TTGAGAAGTC AGCAGGGGAT GTGGACGCCC TGGCCTTTGA CGGACAGACC
    TACGTCGAGT ACCTCAATGC CGTGACTGAG AGCGAACTGA CCAATGAGAT CCCAGCTCCT
    GATGCTCCGG ATTCCGGGGC CCTTCCCAGT GAGAAGGCCC TGCAGAGCAA CCACTTTGAG
    CTGAGCCTGC GCACGGAAGC CACTCAGGGG CTGGTGCTGT GGAGCGGCAA GGCCACGGAG
    CGGGCCGACT ATGTCGCACT GGCCATCGTG GATGGGCGCC TGCAGCTGAC CTATGACCTG
    GGCTCCCAGC CCGTCGTGCT GCGCTCCACC GTGCCGGTCA ACACCAACCG CTGGCTACGA
    GTCAGGGCAC ATAGGGAACA GAGGGAAGGT TCCCTTCAGG TGGGCAATGA GGCTCCCGTG
    ACTGGCTCTT CCCCGCTGGG TGCCACACAG CTAGACACAG ACGGAACTCT ATGGCTGGGT
    GGCCTGGAGA AGCTGCCCAT AGGCCAGGCC CTGCCCAAGG CCTATGGCAC GGGCTTTGTG
    GGCTGCCTGC GGGATGTGGT GGTGGGGCAG CGCCCACTGC ACCTGCTGGA GGATGCTGTC
    ACCAAGCCAG AGCTCCGACC CTGTGCCGCC CCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06695
    Clone ID Related Accession (Same CDS sequence) XM_005620361.3 , XM_005620361.2
    Accession Version XM_005620361.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6210bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product agrin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005620361.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
    GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG
    GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC
    CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC
    ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA
    GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC
    CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG
    ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC
    TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC
    AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT
    CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC
    TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG
    CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC
    CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC
    CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG
    CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG
    GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC
    CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG
    TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT
    GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT
    GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG
    GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA
    TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT
    GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG
    GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG
    TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT
    GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC
    TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT
    GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC
    TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG
    GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG
    GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG
    GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT
    GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG
    CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG
    CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC
    ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT
    GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT
    GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC
    TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG
    CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT
    GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT
    GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGAGA CTGTTGCTCC TGGGGCCCGC
    CCAACATCTG TACCCAGGGC CACCTCAGGG CTCAGCACGA GCAAGGCGCT GCCACCCGCC
    CCCAGCACCC TCCCCCTGGC TCCCAGCAGC ACCCCTCATA GCCGGCCCAC GCCTCGACCC
    TCACCAGGAT CTCGGACCAC TGCCAGCATT CCCAGGACTG TCACGAGGCC TGTGCTGACC
    ATGCCCCCCA CAGCCCCCGC CACGGCAGCC AACCTCGCCA CATCTGCTTT TGGCGAGTCT
    GGCAGTGCTG ACGGGAGCGG CGATGAGGGG CTGAGTGGTG ACCTGGAGGC CAGTGGGACT
    GGCTCAGGGG GACTCGAGCC CCCTGAAGGT GGCAGTGCTG TGACCCCTGA GCCACCCATG
    GAGAGGGCTT CTTGCTACAA CTCGCCTTTG GGCTGCTGCT CGGATGGCAA GACGCCCTCG
    CTGGACGCAG AGGGTTCCAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GTTGGAGCTT
    GAGGGCGTGG AGGGGCAAGA GCTGTTCTAC ACGCCGGAGA TGGCTGACCC CAAGTCAGAG
    CTTTTTGGGG AGACTGCTAG GAGCATCGAG AGCACCCTGG ATGACCTCTT CCGGAACTCA
    GAGGTTAAGA AGGACTTCCG AAGCGTCCGG CTGAGGGACT TGGGGCCTGG CAGCTCAGTC
    CGAGCCATTG TGGATGTGCA CTTTGACCCT GCCACAACCT TTAGGGCGCC TGATGTGGGC
    CAAGCCCTGC TCCGGCAGAT ACAGGCATCC CGGCGCCGGT CCCTGGGCGT ACGGCGGCCA
    CTGCAGGAGC ACGTGCGCTT TATGGACTTT GACTGGTTTC CTGCGTTCTT CACGGGAGCC
    ACCACAGGAG CAACACCCGC CGCAGCCACG GCCAGAGCCA CCACGGTGGC CCACCTGCCA
    CCTGCTGGGA CCCAGTGGGC CCTCTACCCC AGTCACACAA GCCGGCCTGT GGGCAGGACC
    ACAGCTCCAC CCACCACACG CCAGCCTCCC ACCACTGCTC CCCGTCGCGT GCCCGGACGC
    CGGCCCTTGC CCCCAGGCAC CCAGCAGCCC CCGAAGCCCT GCGACTCCCA GCCCTGCCTC
    CATGGGGGAA CCTGCCAGGA CCAGGACTCG GGTGGAGGCT TCACCTGCAG CTGCCCAGTG
    GGCAGGAGGG GTGCCGTCTG TGAGAAGGCC CTGTACCTCT CGGTGCCAGC TTTCAATGGC
    CGCTCCTTCC TGGCCTTCCC CACCCTCCGT GCCTACCACA CGCTGCGCCT AGCACTTGAG
    TTCCGGGCAC TAGAGCCACA GGGCCTGCTG CTCTATAATG GCAATGCCCA GGGCAAGGAC
    TTCCTGGCAT TGACGCTGCT GGGGGGCCGT GTGCAGCTCA GGTTTGACAC AGGTTCCGGG
    CCTGCAGTAC TGACCAGCTC AGTGCCTATA GAGCCTGGCC GCTGGCATCG CTTGGAGCTG
    TCTCGGCACT GGCGCCGGGG AACACTTTCA GTGGATGGTG AGCCCCTGGT GCTGGGCCAC
    AGCCCCAGCG GTACAGATGG TCTCAACCTG GACACAGACC TTTTTGTGGG CGGCATCCCT
    GAGGATCAGG CTTCTGTGGT ACTCGAAAGG ACGTCCATCA GCGTTGGCCT CAAGGGCTGC
    ATCCGCCTGC TGGATGTCAA TAATCAGCGT TTAGAGCTCA GCAGCTGGCC AGGGGCTGCA
    GCCCGAAGCT CCGGGGTGGG CGAGTGTGGA GACCACCCCT GCCTGCCCAA CCCCTGCCTC
    GACGGGGCTC CGTGCCAGGC CCTGGAGGCT GGCATGTTCC ACTGTCAGTG CCCACCTGGC
    CGCTTTGGTC CAACCTGTAG TGGTGACAAG AACCCCTGTG AGCCGAACCC CTGCCATGGG
    GCAGCCCCCT GCCGTGTGCT GCCTGATGGG GAGGCCAAGT GTGAATGCCC CCGAGGACGA
    GGGGGTGCCA TCTGCCAGAC AGTCTCGGAG CGGGATTCTT CCCAGCCCTT CCTGGCTGAC
    TTCCATGGCT TCTCCTACCT GGAGCTGAAA GGGTTGCATA CCTTTGAGCG GGACCTGGGG
    GAGAAGCTGG CACTGGAGGT GGTGTTCCTG GCCCGTGGGC CCAGTGGTTT GCTCCTCTAC
    AATGGGCAGA AAACAGACGG CAAGGGGGAC TTTGTGTCAC TGGCGTTGCA TGACCGCTTC
    TTGGAATTCC GCTATGACCT GGGCAAGGGA GCAGCGGTCA TCAGGAGCAA GGAGCCGGTG
    GCCCTGGACA CCTGGACCAG GGTCTCACTA GAGCGCAATG GCCGCAAGGG TGCCATGCGT
    GTCAGTGATG GGCCTCGCGT GCTGGGAGAG TCCCCGAAAT CCCGCAAGGT GCCACACACT
    GTCCTCAACC TGAAGGAGCC ACTCTATGTT GGAGGGGCCC CTGACTTCAG CAAACTGGCC
    CGGGCAGCTG CGGTATTCTC TGGCTTCGAT GGCGCCATCC AACTGGTCTC CTTGAAAGGC
    CACCAGCTGC TGTCCCAGGA GCACGTGGTG CGGTCAGTGG ATGTCTCATC TTTTGCCCAC
    CACCCTTGTA CTCAGGCCTC GGGCCACCCA TGCCTCAATG GGGCCTCCTG CCTCCCTCGA
    GAGGCCTCCT ATGAGTGCCT GTGCCCCGGG GGCTTCTCAG GGCTTCACTG TGAGAAGGGG
    CTGATTGAGA AGTCAGCAGG GGATGTGGAC GCCCTGGCCT TTGACGGACA GACCTACGTC
    GAGTACCTCA ATGCCGTGAC TGAGAGCGAA CTGACCAATG AGATCCCAGC TCCTGATGCT
    CCGGATTCCG GGGCCCTTCC CAGTGAGAAG GCCCTGCAGA GCAACCACTT TGAGCTGAGC
    CTGCGCACGG AAGCCACTCA GGGGCTGGTG CTGTGGAGCG GCAAGGCCAC GGAGCGGGCC
    GACTATGTCG CACTGGCCAT CGTGGATGGG CGCCTGCAGC TGACCTATGA CCTGGGCTCC
    CAGCCCGTCG TGCTGCGCTC CACCGTGCCG GTCAACACCA ACCGCTGGCT ACGAGTCAGG
    GCACATAGGG AACAGAGGGA AGGTTCCCTT CAGGTGGGCA ATGAGGCTCC CGTGACTGGC
    TCTTCCCCGC TGGGTGCCAC ACAGCTAGAC ACAGACGGAA CTCTATGGCT GGGTGGCCTG
    GAGAAGCTGC CCATAGGCCA GGCCCTGCCC AAGGCCTATG GCACGGGCTT TGTGGGCTGC
    CTGCGGGATG TGGTGGTGGG GCAGCGCCCA CTGCACCTGC TGGAGGATGC TGTCACCAAG
    CCAGAGCTCC GACCCTGTGC CGCCCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005620418.1
    CDS1..6210
    Translation

    Target ORF information:

    RefSeq Version XM_005620361.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris agrin (AGRN), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005620361.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
    GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG
    GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC
    CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC
    ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA
    GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC
    CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG
    ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC
    TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC
    AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT
    CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC
    TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG
    CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC
    CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC
    CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG
    CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG
    GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC
    CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG
    TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT
    GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT
    GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG
    GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA
    TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT
    GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG
    GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG
    TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT
    GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC
    TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT
    GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC
    TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG
    GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG
    GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG
    GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT
    GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG
    CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG
    CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC
    ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT
    GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT
    GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC
    TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG
    CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT
    GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT
    GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGAGA CTGTTGCTCC TGGGGCCCGC
    CCAACATCTG TACCCAGGGC CACCTCAGGG CTCAGCACGA GCAAGGCGCT GCCACCCGCC
    CCCAGCACCC TCCCCCTGGC TCCCAGCAGC ACCCCTCATA GCCGGCCCAC GCCTCGACCC
    TCACCAGGAT CTCGGACCAC TGCCAGCATT CCCAGGACTG TCACGAGGCC TGTGCTGACC
    ATGCCCCCCA CAGCCCCCGC CACGGCAGCC AACCTCGCCA CATCTGCTTT TGGCGAGTCT
    GGCAGTGCTG ACGGGAGCGG CGATGAGGGG CTGAGTGGTG ACCTGGAGGC CAGTGGGACT
    GGCTCAGGGG GACTCGAGCC CCCTGAAGGT GGCAGTGCTG TGACCCCTGA GCCACCCATG
    GAGAGGGCTT CTTGCTACAA CTCGCCTTTG GGCTGCTGCT CGGATGGCAA GACGCCCTCG
    CTGGACGCAG AGGGTTCCAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GTTGGAGCTT
    GAGGGCGTGG AGGGGCAAGA GCTGTTCTAC ACGCCGGAGA TGGCTGACCC CAAGTCAGAG
    CTTTTTGGGG AGACTGCTAG GAGCATCGAG AGCACCCTGG ATGACCTCTT CCGGAACTCA
    GAGGTTAAGA AGGACTTCCG AAGCGTCCGG CTGAGGGACT TGGGGCCTGG CAGCTCAGTC
    CGAGCCATTG TGGATGTGCA CTTTGACCCT GCCACAACCT TTAGGGCGCC TGATGTGGGC
    CAAGCCCTGC TCCGGCAGAT ACAGGCATCC CGGCGCCGGT CCCTGGGCGT ACGGCGGCCA
    CTGCAGGAGC ACGTGCGCTT TATGGACTTT GACTGGTTTC CTGCGTTCTT CACGGGAGCC
    ACCACAGGAG CAACACCCGC CGCAGCCACG GCCAGAGCCA CCACGGTGGC CCACCTGCCA
    CCTGCTGGGA CCCAGTGGGC CCTCTACCCC AGTCACACAA GCCGGCCTGT GGGCAGGACC
    ACAGCTCCAC CCACCACACG CCAGCCTCCC ACCACTGCTC CCCGTCGCGT GCCCGGACGC
    CGGCCCTTGC CCCCAGGCAC CCAGCAGCCC CCGAAGCCCT GCGACTCCCA GCCCTGCCTC
    CATGGGGGAA CCTGCCAGGA CCAGGACTCG GGTGGAGGCT TCACCTGCAG CTGCCCAGTG
    GGCAGGAGGG GTGCCGTCTG TGAGAAGGCC CTGTACCTCT CGGTGCCAGC TTTCAATGGC
    CGCTCCTTCC TGGCCTTCCC CACCCTCCGT GCCTACCACA CGCTGCGCCT AGCACTTGAG
    TTCCGGGCAC TAGAGCCACA GGGCCTGCTG CTCTATAATG GCAATGCCCA GGGCAAGGAC
    TTCCTGGCAT TGACGCTGCT GGGGGGCCGT GTGCAGCTCA GGTTTGACAC AGGTTCCGGG
    CCTGCAGTAC TGACCAGCTC AGTGCCTATA GAGCCTGGCC GCTGGCATCG CTTGGAGCTG
    TCTCGGCACT GGCGCCGGGG AACACTTTCA GTGGATGGTG AGCCCCTGGT GCTGGGCCAC
    AGCCCCAGCG GTACAGATGG TCTCAACCTG GACACAGACC TTTTTGTGGG CGGCATCCCT
    GAGGATCAGG CTTCTGTGGT ACTCGAAAGG ACGTCCATCA GCGTTGGCCT CAAGGGCTGC
    ATCCGCCTGC TGGATGTCAA TAATCAGCGT TTAGAGCTCA GCAGCTGGCC AGGGGCTGCA
    GCCCGAAGCT CCGGGGTGGG CGAGTGTGGA GACCACCCCT GCCTGCCCAA CCCCTGCCTC
    GACGGGGCTC CGTGCCAGGC CCTGGAGGCT GGCATGTTCC ACTGTCAGTG CCCACCTGGC
    CGCTTTGGTC CAACCTGTAG TGGTGACAAG AACCCCTGTG AGCCGAACCC CTGCCATGGG
    GCAGCCCCCT GCCGTGTGCT GCCTGATGGG GAGGCCAAGT GTGAATGCCC CCGAGGACGA
    GGGGGTGCCA TCTGCCAGAC AGTCTCGGAG CGGGATTCTT CCCAGCCCTT CCTGGCTGAC
    TTCCATGGCT TCTCCTACCT GGAGCTGAAA GGGTTGCATA CCTTTGAGCG GGACCTGGGG
    GAGAAGCTGG CACTGGAGGT GGTGTTCCTG GCCCGTGGGC CCAGTGGTTT GCTCCTCTAC
    AATGGGCAGA AAACAGACGG CAAGGGGGAC TTTGTGTCAC TGGCGTTGCA TGACCGCTTC
    TTGGAATTCC GCTATGACCT GGGCAAGGGA GCAGCGGTCA TCAGGAGCAA GGAGCCGGTG
    GCCCTGGACA CCTGGACCAG GGTCTCACTA GAGCGCAATG GCCGCAAGGG TGCCATGCGT
    GTCAGTGATG GGCCTCGCGT GCTGGGAGAG TCCCCGAAAT CCCGCAAGGT GCCACACACT
    GTCCTCAACC TGAAGGAGCC ACTCTATGTT GGAGGGGCCC CTGACTTCAG CAAACTGGCC
    CGGGCAGCTG CGGTATTCTC TGGCTTCGAT GGCGCCATCC AACTGGTCTC CTTGAAAGGC
    CACCAGCTGC TGTCCCAGGA GCACGTGGTG CGGTCAGTGG ATGTCTCATC TTTTGCCCAC
    CACCCTTGTA CTCAGGCCTC GGGCCACCCA TGCCTCAATG GGGCCTCCTG CCTCCCTCGA
    GAGGCCTCCT ATGAGTGCCT GTGCCCCGGG GGCTTCTCAG GGCTTCACTG TGAGAAGGGG
    CTGATTGAGA AGTCAGCAGG GGATGTGGAC GCCCTGGCCT TTGACGGACA GACCTACGTC
    GAGTACCTCA ATGCCGTGAC TGAGAGCGAA CTGACCAATG AGATCCCAGC TCCTGATGCT
    CCGGATTCCG GGGCCCTTCC CAGTGAGAAG GCCCTGCAGA GCAACCACTT TGAGCTGAGC
    CTGCGCACGG AAGCCACTCA GGGGCTGGTG CTGTGGAGCG GCAAGGCCAC GGAGCGGGCC
    GACTATGTCG CACTGGCCAT CGTGGATGGG CGCCTGCAGC TGACCTATGA CCTGGGCTCC
    CAGCCCGTCG TGCTGCGCTC CACCGTGCCG GTCAACACCA ACCGCTGGCT ACGAGTCAGG
    GCACATAGGG AACAGAGGGA AGGTTCCCTT CAGGTGGGCA ATGAGGCTCC CGTGACTGGC
    TCTTCCCCGC TGGGTGCCAC ACAGCTAGAC ACAGACGGAA CTCTATGGCT GGGTGGCCTG
    GAGAAGCTGC CCATAGGCCA GGCCCTGCCC AAGGCCTATG GCACGGGCTT TGTGGGCTGC
    CTGCGGGATG TGGTGGTGGG GCAGCGCCCA CTGCACCTGC TGGAGGATGC TGTCACCAAG
    CCAGAGCTCC GACCCTGTGC CGCCCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06695
    Clone ID Related Accession (Same CDS sequence) XM_005620361.3 , XM_005620361.2
    Accession Version XM_005620361.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6210bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product agrin isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726058.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005620361.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
    GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG
    GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC
    CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC
    ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA
    GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC
    CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG
    ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC
    TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC
    AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT
    CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC
    TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG
    CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC
    CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC
    CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG
    CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG
    GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC
    CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG
    TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT
    GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT
    GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG
    GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA
    TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT
    GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG
    GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG
    TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT
    GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC
    TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT
    GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC
    TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG
    GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG
    GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG
    GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT
    GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG
    CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG
    CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC
    ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT
    GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT
    GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC
    TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG
    CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT
    GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT
    GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGAGA CTGTTGCTCC TGGGGCCCGC
    CCAACATCTG TACCCAGGGC CACCTCAGGG CTCAGCACGA GCAAGGCGCT GCCACCCGCC
    CCCAGCACCC TCCCCCTGGC TCCCAGCAGC ACCCCTCATA GCCGGCCCAC GCCTCGACCC
    TCACCAGGAT CTCGGACCAC TGCCAGCATT CCCAGGACTG TCACGAGGCC TGTGCTGACC
    ATGCCCCCCA CAGCCCCCGC CACGGCAGCC AACCTCGCCA CATCTGCTTT TGGCGAGTCT
    GGCAGTGCTG ACGGGAGCGG CGATGAGGGG CTGAGTGGTG ACCTGGAGGC CAGTGGGACT
    GGCTCAGGGG GACTCGAGCC CCCTGAAGGT GGCAGTGCTG TGACCCCTGA GCCACCCATG
    GAGAGGGCTT CTTGCTACAA CTCGCCTTTG GGCTGCTGCT CGGATGGCAA GACGCCCTCG
    CTGGACGCAG AGGGTTCCAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GTTGGAGCTT
    GAGGGCGTGG AGGGGCAAGA GCTGTTCTAC ACGCCGGAGA TGGCTGACCC CAAGTCAGAG
    CTTTTTGGGG AGACTGCTAG GAGCATCGAG AGCACCCTGG ATGACCTCTT CCGGAACTCA
    GAGGTTAAGA AGGACTTCCG AAGCGTCCGG CTGAGGGACT TGGGGCCTGG CAGCTCAGTC
    CGAGCCATTG TGGATGTGCA CTTTGACCCT GCCACAACCT TTAGGGCGCC TGATGTGGGC
    CAAGCCCTGC TCCGGCAGAT ACAGGCATCC CGGCGCCGGT CCCTGGGCGT ACGGCGGCCA
    CTGCAGGAGC ACGTGCGCTT TATGGACTTT GACTGGTTTC CTGCGTTCTT CACGGGAGCC
    ACCACAGGAG CAACACCCGC CGCAGCCACG GCCAGAGCCA CCACGGTGGC CCACCTGCCA
    CCTGCTGGGA CCCAGTGGGC CCTCTACCCC AGTCACACAA GCCGGCCTGT GGGCAGGACC
    ACAGCTCCAC CCACCACACG CCAGCCTCCC ACCACTGCTC CCCGTCGCGT GCCCGGACGC
    CGGCCCTTGC CCCCAGGCAC CCAGCAGCCC CCGAAGCCCT GCGACTCCCA GCCCTGCCTC
    CATGGGGGAA CCTGCCAGGA CCAGGACTCG GGTGGAGGCT TCACCTGCAG CTGCCCAGTG
    GGCAGGAGGG GTGCCGTCTG TGAGAAGGCC CTGTACCTCT CGGTGCCAGC TTTCAATGGC
    CGCTCCTTCC TGGCCTTCCC CACCCTCCGT GCCTACCACA CGCTGCGCCT AGCACTTGAG
    TTCCGGGCAC TAGAGCCACA GGGCCTGCTG CTCTATAATG GCAATGCCCA GGGCAAGGAC
    TTCCTGGCAT TGACGCTGCT GGGGGGCCGT GTGCAGCTCA GGTTTGACAC AGGTTCCGGG
    CCTGCAGTAC TGACCAGCTC AGTGCCTATA GAGCCTGGCC GCTGGCATCG CTTGGAGCTG
    TCTCGGCACT GGCGCCGGGG AACACTTTCA GTGGATGGTG AGCCCCTGGT GCTGGGCCAC
    AGCCCCAGCG GTACAGATGG TCTCAACCTG GACACAGACC TTTTTGTGGG CGGCATCCCT
    GAGGATCAGG CTTCTGTGGT ACTCGAAAGG ACGTCCATCA GCGTTGGCCT CAAGGGCTGC
    ATCCGCCTGC TGGATGTCAA TAATCAGCGT TTAGAGCTCA GCAGCTGGCC AGGGGCTGCA
    GCCCGAAGCT CCGGGGTGGG CGAGTGTGGA GACCACCCCT GCCTGCCCAA CCCCTGCCTC
    GACGGGGCTC CGTGCCAGGC CCTGGAGGCT GGCATGTTCC ACTGTCAGTG CCCACCTGGC
    CGCTTTGGTC CAACCTGTAG TGGTGACAAG AACCCCTGTG AGCCGAACCC CTGCCATGGG
    GCAGCCCCCT GCCGTGTGCT GCCTGATGGG GAGGCCAAGT GTGAATGCCC CCGAGGACGA
    GGGGGTGCCA TCTGCCAGAC AGTCTCGGAG CGGGATTCTT CCCAGCCCTT CCTGGCTGAC
    TTCCATGGCT TCTCCTACCT GGAGCTGAAA GGGTTGCATA CCTTTGAGCG GGACCTGGGG
    GAGAAGCTGG CACTGGAGGT GGTGTTCCTG GCCCGTGGGC CCAGTGGTTT GCTCCTCTAC
    AATGGGCAGA AAACAGACGG CAAGGGGGAC TTTGTGTCAC TGGCGTTGCA TGACCGCTTC
    TTGGAATTCC GCTATGACCT GGGCAAGGGA GCAGCGGTCA TCAGGAGCAA GGAGCCGGTG
    GCCCTGGACA CCTGGACCAG GGTCTCACTA GAGCGCAATG GCCGCAAGGG TGCCATGCGT
    GTCAGTGATG GGCCTCGCGT GCTGGGAGAG TCCCCGAAAT CCCGCAAGGT GCCACACACT
    GTCCTCAACC TGAAGGAGCC ACTCTATGTT GGAGGGGCCC CTGACTTCAG CAAACTGGCC
    CGGGCAGCTG CGGTATTCTC TGGCTTCGAT GGCGCCATCC AACTGGTCTC CTTGAAAGGC
    CACCAGCTGC TGTCCCAGGA GCACGTGGTG CGGTCAGTGG ATGTCTCATC TTTTGCCCAC
    CACCCTTGTA CTCAGGCCTC GGGCCACCCA TGCCTCAATG GGGCCTCCTG CCTCCCTCGA
    GAGGCCTCCT ATGAGTGCCT GTGCCCCGGG GGCTTCTCAG GGCTTCACTG TGAGAAGGGG
    CTGATTGAGA AGTCAGCAGG GGATGTGGAC GCCCTGGCCT TTGACGGACA GACCTACGTC
    GAGTACCTCA ATGCCGTGAC TGAGAGCGAA CTGACCAATG AGATCCCAGC TCCTGATGCT
    CCGGATTCCG GGGCCCTTCC CAGTGAGAAG GCCCTGCAGA GCAACCACTT TGAGCTGAGC
    CTGCGCACGG AAGCCACTCA GGGGCTGGTG CTGTGGAGCG GCAAGGCCAC GGAGCGGGCC
    GACTATGTCG CACTGGCCAT CGTGGATGGG CGCCTGCAGC TGACCTATGA CCTGGGCTCC
    CAGCCCGTCG TGCTGCGCTC CACCGTGCCG GTCAACACCA ACCGCTGGCT ACGAGTCAGG
    GCACATAGGG AACAGAGGGA AGGTTCCCTT CAGGTGGGCA ATGAGGCTCC CGTGACTGGC
    TCTTCCCCGC TGGGTGCCAC ACAGCTAGAC ACAGACGGAA CTCTATGGCT GGGTGGCCTG
    GAGAAGCTGC CCATAGGCCA GGCCCTGCCC AAGGCCTATG GCACGGGCTT TGTGGGCTGC
    CTGCGGGATG TGGTGGTGGG GCAGCGCCCA CTGCACCTGC TGGAGGATGC TGTCACCAAG
    CCAGAGCTCC GACCCTGTGC CGCCCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005620418.1
    CDS133..6342
    Translation

    Target ORF information:

    RefSeq Version XM_005620361.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris agrin (AGRN), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005620361.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
    GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG
    GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC
    CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC
    ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA
    GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC
    CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG
    ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC
    TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC
    AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT
    CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC
    TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG
    CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC
    CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC
    CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG
    CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG
    GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC
    CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG
    TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT
    GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT
    GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG
    GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA
    TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT
    GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG
    GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG
    TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT
    GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC
    TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT
    GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC
    TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG
    GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG
    GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG
    GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT
    GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG
    CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG
    CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC
    ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT
    GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT
    GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC
    TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG
    CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT
    GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT
    GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGAGA CTGTTGCTCC TGGGGCCCGC
    CCAACATCTG TACCCAGGGC CACCTCAGGG CTCAGCACGA GCAAGGCGCT GCCACCCGCC
    CCCAGCACCC TCCCCCTGGC TCCCAGCAGC ACCCCTCATA GCCGGCCCAC GCCTCGACCC
    TCACCAGGAT CTCGGACCAC TGCCAGCATT CCCAGGACTG TCACGAGGCC TGTGCTGACC
    ATGCCCCCCA CAGCCCCCGC CACGGCAGCC AACCTCGCCA CATCTGCTTT TGGCGAGTCT
    GGCAGTGCTG ACGGGAGCGG CGATGAGGGG CTGAGTGGTG ACCTGGAGGC CAGTGGGACT
    GGCTCAGGGG GACTCGAGCC CCCTGAAGGT GGCAGTGCTG TGACCCCTGA GCCACCCATG
    GAGAGGGCTT CTTGCTACAA CTCGCCTTTG GGCTGCTGCT CGGATGGCAA GACGCCCTCG
    CTGGACGCAG AGGGTTCCAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GTTGGAGCTT
    GAGGGCGTGG AGGGGCAAGA GCTGTTCTAC ACGCCGGAGA TGGCTGACCC CAAGTCAGAG
    CTTTTTGGGG AGACTGCTAG GAGCATCGAG AGCACCCTGG ATGACCTCTT CCGGAACTCA
    GAGGTTAAGA AGGACTTCCG AAGCGTCCGG CTGAGGGACT TGGGGCCTGG CAGCTCAGTC
    CGAGCCATTG TGGATGTGCA CTTTGACCCT GCCACAACCT TTAGGGCGCC TGATGTGGGC
    CAAGCCCTGC TCCGGCAGAT ACAGGCATCC CGGCGCCGGT CCCTGGGCGT ACGGCGGCCA
    CTGCAGGAGC ACGTGCGCTT TATGGACTTT GACTGGTTTC CTGCGTTCTT CACGGGAGCC
    ACCACAGGAG CAACACCCGC CGCAGCCACG GCCAGAGCCA CCACGGTGGC CCACCTGCCA
    CCTGCTGGGA CCCAGTGGGC CCTCTACCCC AGTCACACAA GCCGGCCTGT GGGCAGGACC
    ACAGCTCCAC CCACCACACG CCAGCCTCCC ACCACTGCTC CCCGTCGCGT GCCCGGACGC
    CGGCCCTTGC CCCCAGGCAC CCAGCAGCCC CCGAAGCCCT GCGACTCCCA GCCCTGCCTC
    CATGGGGGAA CCTGCCAGGA CCAGGACTCG GGTGGAGGCT TCACCTGCAG CTGCCCAGTG
    GGCAGGAGGG GTGCCGTCTG TGAGAAGGCC CTGTACCTCT CGGTGCCAGC TTTCAATGGC
    CGCTCCTTCC TGGCCTTCCC CACCCTCCGT GCCTACCACA CGCTGCGCCT AGCACTTGAG
    TTCCGGGCAC TAGAGCCACA GGGCCTGCTG CTCTATAATG GCAATGCCCA GGGCAAGGAC
    TTCCTGGCAT TGACGCTGCT GGGGGGCCGT GTGCAGCTCA GGTTTGACAC AGGTTCCGGG
    CCTGCAGTAC TGACCAGCTC AGTGCCTATA GAGCCTGGCC GCTGGCATCG CTTGGAGCTG
    TCTCGGCACT GGCGCCGGGG AACACTTTCA GTGGATGGTG AGCCCCTGGT GCTGGGCCAC
    AGCCCCAGCG GTACAGATGG TCTCAACCTG GACACAGACC TTTTTGTGGG CGGCATCCCT
    GAGGATCAGG CTTCTGTGGT ACTCGAAAGG ACGTCCATCA GCGTTGGCCT CAAGGGCTGC
    ATCCGCCTGC TGGATGTCAA TAATCAGCGT TTAGAGCTCA GCAGCTGGCC AGGGGCTGCA
    GCCCGAAGCT CCGGGGTGGG CGAGTGTGGA GACCACCCCT GCCTGCCCAA CCCCTGCCTC
    GACGGGGCTC CGTGCCAGGC CCTGGAGGCT GGCATGTTCC ACTGTCAGTG CCCACCTGGC
    CGCTTTGGTC CAACCTGTAG TGGTGACAAG AACCCCTGTG AGCCGAACCC CTGCCATGGG
    GCAGCCCCCT GCCGTGTGCT GCCTGATGGG GAGGCCAAGT GTGAATGCCC CCGAGGACGA
    GGGGGTGCCA TCTGCCAGAC AGTCTCGGAG CGGGATTCTT CCCAGCCCTT CCTGGCTGAC
    TTCCATGGCT TCTCCTACCT GGAGCTGAAA GGGTTGCATA CCTTTGAGCG GGACCTGGGG
    GAGAAGCTGG CACTGGAGGT GGTGTTCCTG GCCCGTGGGC CCAGTGGTTT GCTCCTCTAC
    AATGGGCAGA AAACAGACGG CAAGGGGGAC TTTGTGTCAC TGGCGTTGCA TGACCGCTTC
    TTGGAATTCC GCTATGACCT GGGCAAGGGA GCAGCGGTCA TCAGGAGCAA GGAGCCGGTG
    GCCCTGGACA CCTGGACCAG GGTCTCACTA GAGCGCAATG GCCGCAAGGG TGCCATGCGT
    GTCAGTGATG GGCCTCGCGT GCTGGGAGAG TCCCCGAAAT CCCGCAAGGT GCCACACACT
    GTCCTCAACC TGAAGGAGCC ACTCTATGTT GGAGGGGCCC CTGACTTCAG CAAACTGGCC
    CGGGCAGCTG CGGTATTCTC TGGCTTCGAT GGCGCCATCC AACTGGTCTC CTTGAAAGGC
    CACCAGCTGC TGTCCCAGGA GCACGTGGTG CGGTCAGTGG ATGTCTCATC TTTTGCCCAC
    CACCCTTGTA CTCAGGCCTC GGGCCACCCA TGCCTCAATG GGGCCTCCTG CCTCCCTCGA
    GAGGCCTCCT ATGAGTGCCT GTGCCCCGGG GGCTTCTCAG GGCTTCACTG TGAGAAGGGG
    CTGATTGAGA AGTCAGCAGG GGATGTGGAC GCCCTGGCCT TTGACGGACA GACCTACGTC
    GAGTACCTCA ATGCCGTGAC TGAGAGCGAA CTGACCAATG AGATCCCAGC TCCTGATGCT
    CCGGATTCCG GGGCCCTTCC CAGTGAGAAG GCCCTGCAGA GCAACCACTT TGAGCTGAGC
    CTGCGCACGG AAGCCACTCA GGGGCTGGTG CTGTGGAGCG GCAAGGCCAC GGAGCGGGCC
    GACTATGTCG CACTGGCCAT CGTGGATGGG CGCCTGCAGC TGACCTATGA CCTGGGCTCC
    CAGCCCGTCG TGCTGCGCTC CACCGTGCCG GTCAACACCA ACCGCTGGCT ACGAGTCAGG
    GCACATAGGG AACAGAGGGA AGGTTCCCTT CAGGTGGGCA ATGAGGCTCC CGTGACTGGC
    TCTTCCCCGC TGGGTGCCAC ACAGCTAGAC ACAGACGGAA CTCTATGGCT GGGTGGCCTG
    GAGAAGCTGC CCATAGGCCA GGCCCTGCCC AAGGCCTATG GCACGGGCTT TGTGGGCTGC
    CTGCGGGATG TGGTGGTGGG GCAGCGCCCA CTGCACCTGC TGGAGGATGC TGTCACCAAG
    CCAGAGCTCC GACCCTGTGC CGCCCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06696
    Clone ID Related Accession (Same CDS sequence) XM_005620362.2 , XM_005620362.3
    Accession Version XM_005620362.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6195bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product agrin isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726058.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005620362.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
    GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG
    GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC
    CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC
    ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA
    GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC
    CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG
    ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC
    TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC
    AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT
    CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC
    TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG
    CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC
    CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC
    CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG
    CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG
    GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC
    CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG
    TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT
    GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT
    GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG
    GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA
    TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT
    GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG
    GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG
    TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT
    GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC
    TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT
    GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC
    TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG
    GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG
    GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG
    GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT
    GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG
    CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG
    CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC
    ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT
    GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT
    GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC
    TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG
    CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT
    GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT
    GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGACT CTGTTCTCCT TGCAGAGACT
    GTTGCTCCTG GGGCCCGCCC AACATCTGTA CCCAGGGCCA CCTCAGGGCT CAGCACGAGC
    AAGGCGCTGC CACCCGCCCC CAGCACCCTC CCCCTGGCTC CCAGCAGCAC CCCTCATAGC
    CGGCCCACGC CTCGACCCTC ACCAGGATCT CGGACCACTG CCAGCATTCC CAGGACTGTC
    ACGAGGCCTG TGCTGACCAT GCCCCCCACA GCCCCCGCCA CGGCAGCCAA CCTCGCCACA
    TCTGCTTTTG GCGAGTCTGG CAGTGCTGAC GGGAGCGGCG ATGAGGGGCT GAGTGGTGAC
    CTGGAGGCCA GTGGGACTGG CTCAGGGGGA CTCGAGCCCC CTGAAGGTGG CAGTGCTGTG
    ACCCCTGAGC CACCCATGGA GAGGGCTTCT TGCTACAACT CGCCTTTGGG CTGCTGCTCG
    GATGGCAAGA CGCCCTCGCT GGACGCAGAG GGTTCCAACT GTCCTGCCAC CAAGGCGTTC
    CAGGGTGTGT TGGAGCTTGA GGGCGTGGAG GGGCAAGAGC TGTTCTACAC GCCGGAGATG
    GCTGACCCCA AGTCAGAGCT TTTTGGGGAG ACTGCTAGGA GCATCGAGAG CACCCTGGAT
    GACCTCTTCC GGAACTCAGA GGTTAAGAAG GACTTCCGAA GCGTCCGGCT GAGGGACTTG
    GGGCCTGGCA GCTCAGTCCG AGCCATTGTG GATGTGCACT TTGACCCTGC CACAACCTTT
    AGGGCGCCTG ATGTGGGCCA AGCCCTGCTC CGGCAGATAC AGGCATCCCG GCGCCGGTCC
    CTGGGCGTAC GGCGGCCACT GCAGGAGCAC GTGCGCTTTA TGGACTTTGA CTGGTTTCCT
    GCGTTCTTCA CGGGAGCCAC CACAGGAGCA ACACCCGCCG CAGCCACGGC CAGAGCCACC
    ACGGTGGCCC ACCTGCCACC TGCTGGGACC CAGTGGGCCC TCTACCCCAG TCACACAAGC
    CGGCCTGTGG GCAGGACCAC AGCTCCACCC ACCACACGCC AGCCTCCCAC CACTGCTCCC
    CGTCGCGTGC CCGGACGCCG GCCCTTGCCC CCAGGCACCC AGCAGCCCCC GAAGCCCTGC
    GACTCCCAGC CCTGCCTCCA TGGGGGAACC TGCCAGGACC AGGACTCGGG TGGAGGCTTC
    ACCTGCAGCT GCCCAGTGGG CAGGAGGGGT GCCGTCTGTG AGAAGGCCCT GTACCTCTCG
    GTGCCAGCTT TCAATGGCCG CTCCTTCCTG GCCTTCCCCA CCCTCCGTGC CTACCACACG
    CTGCGCCTAG CACTTGAGTT CCGGGCACTA GAGCCACAGG GCCTGCTGCT CTATAATGGC
    AATGCCCAGG GCAAGGACTT CCTGGCATTG ACGCTGCTGG GGGGCCGTGT GCAGCTCAGG
    TTTGACACAG GTTCCGGGCC TGCAGTACTG ACCAGCTCAG TGCCTATAGA GCCTGGCCGC
    TGGCATCGCT TGGAGCTGTC TCGGCACTGG CGCCGGGGAA CACTTTCAGT GGATGGTGAG
    CCCCTGGTGC TGGGCCACAG CCCCAGCGGT ACAGATGGTC TCAACCTGGA CACAGACCTT
    TTTGTGGGCG GCATCCCTGA GGATCAGGCT TCTGTGGTAC TCGAAAGGAC GTCCATCAGC
    GTTGGCCTCA AGGGCTGCAT CCGCCTGCTG GATGTCAATA ATCAGCGTTT AGAGCTCAGC
    AGCTGGCCAG GGGCTGCAGC CCGAAGCTCC GGGGTGGGCG AGTGTGGAGA CCACCCCTGC
    CTGCCCAACC CCTGCCTCGA CGGGGCTCCG TGCCAGGCCC TGGAGGCTGG CATGTTCCAC
    TGTCAGTGCC CACCTGGCCG CTTTGGTCCA ACCTGTAGTG GTGACAAGAA CCCCTGTGAG
    CCGAACCCCT GCCATGGGGC AGCCCCCTGC CGTGTGCTGC CTGATGGGGA GGCCAAGTGT
    GAATGCCCCC GAGGACGAGG GGGTGCCATC TGCCAGACAG TCTCGGAGCG GGATTCTTCC
    CAGCCCTTCC TGGCTGACTT CCATGGCTTC TCCTACCTGG AGCTGAAAGG GTTGCATACC
    TTTGAGCGGG ACCTGGGGGA GAAGCTGGCA CTGGAGGTGG TGTTCCTGGC CCGTGGGCCC
    AGTGGTTTGC TCCTCTACAA TGGGCAGAAA ACAGACGGCA AGGGGGACTT TGTGTCACTG
    GCGTTGCATG ACCGCTTCTT GGAATTCCGC TATGACCTGG GCAAGGGAGC AGCGGTCATC
    AGGAGCAAGG AGCCGGTGGC CCTGGACACC TGGACCAGGG TCTCACTAGA GCGCAATGGC
    CGCAAGGGTG CCATGCGTGT CAGTGATGGG CCTCGCGTGC TGGGAGAGTC CCCGAAATCC
    CGCAAGGTGC CACACACTGT CCTCAACCTG AAGGAGCCAC TCTATGTTGG AGGGGCCCCT
    GACTTCAGCA AACTGGCCCG GGCAGCTGCG GTATTCTCTG GCTTCGATGG CGCCATCCAA
    CTGGTCTCCT TGAAAGGCCA CCAGCTGCTG TCCCAGGAGC ACGTGGTGCG GTCAGTGGAT
    GTCTCATCTT TTGCCCACCA CCCTTGTACT CAGGCCTCGG GCCACCCATG CCTCAATGGG
    GCCTCCTGCC TCCCTCGAGA GGCCTCCTAT GAGTGCCTGT GCCCCGGGGG CTTCTCAGGG
    CTTCACTGTG AGAAGGGGCT GATTGAGAAG TCAGCAGGGG ATGTGGACGC CCTGGCCTTT
    GACGGACAGA CCTACGTCGA GTACCTCAAT GCCGTGACTG AGAGCGAACT GACCAATGAG
    ATCCCAGCTG AGAAGGCCCT GCAGAGCAAC CACTTTGAGC TGAGCCTGCG CACGGAAGCC
    ACTCAGGGGC TGGTGCTGTG GAGCGGCAAG GCCACGGAGC GGGCCGACTA TGTCGCACTG
    GCCATCGTGG ATGGGCGCCT GCAGCTGACC TATGACCTGG GCTCCCAGCC CGTCGTGCTG
    CGCTCCACCG TGCCGGTCAA CACCAACCGC TGGCTACGAG TCAGGGCACA TAGGGAACAG
    AGGGAAGGTT CCCTTCAGGT GGGCAATGAG GCTCCCGTGA CTGGCTCTTC CCCGCTGGGT
    GCCACACAGC TAGACACAGA CGGAACTCTA TGGCTGGGTG GCCTGGAGAA GCTGCCCATA
    GGCCAGGCCC TGCCCAAGGC CTATGGCACG GGCTTTGTGG GCTGCCTGCG GGATGTGGTG
    GTGGGGCAGC GCCCACTGCA CCTGCTGGAG GATGCTGTCA CCAAGCCAGA GCTCCGACCC
    TGTGCCGCCC CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005620419.1
    CDS133..6327
    Translation

    Target ORF information:

    RefSeq Version XM_005620362.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris agrin (AGRN), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005620362.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
    GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG
    GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC
    CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC
    ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA
    GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC
    CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG
    ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC
    TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC
    AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT
    CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC
    TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG
    CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC
    CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC
    CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG
    CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG
    GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC
    CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG
    TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT
    GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT
    GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG
    GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA
    TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT
    GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG
    GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG
    TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT
    GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC
    TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT
    GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC
    TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG
    GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG
    GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG
    GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT
    GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG
    CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG
    CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC
    ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT
    GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT
    GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC
    TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG
    CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT
    GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT
    GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGACT CTGTTCTCCT TGCAGAGACT
    GTTGCTCCTG GGGCCCGCCC AACATCTGTA CCCAGGGCCA CCTCAGGGCT CAGCACGAGC
    AAGGCGCTGC CACCCGCCCC CAGCACCCTC CCCCTGGCTC CCAGCAGCAC CCCTCATAGC
    CGGCCCACGC CTCGACCCTC ACCAGGATCT CGGACCACTG CCAGCATTCC CAGGACTGTC
    ACGAGGCCTG TGCTGACCAT GCCCCCCACA GCCCCCGCCA CGGCAGCCAA CCTCGCCACA
    TCTGCTTTTG GCGAGTCTGG CAGTGCTGAC GGGAGCGGCG ATGAGGGGCT GAGTGGTGAC
    CTGGAGGCCA GTGGGACTGG CTCAGGGGGA CTCGAGCCCC CTGAAGGTGG CAGTGCTGTG
    ACCCCTGAGC CACCCATGGA GAGGGCTTCT TGCTACAACT CGCCTTTGGG CTGCTGCTCG
    GATGGCAAGA CGCCCTCGCT GGACGCAGAG GGTTCCAACT GTCCTGCCAC CAAGGCGTTC
    CAGGGTGTGT TGGAGCTTGA GGGCGTGGAG GGGCAAGAGC TGTTCTACAC GCCGGAGATG
    GCTGACCCCA AGTCAGAGCT TTTTGGGGAG ACTGCTAGGA GCATCGAGAG CACCCTGGAT
    GACCTCTTCC GGAACTCAGA GGTTAAGAAG GACTTCCGAA GCGTCCGGCT GAGGGACTTG
    GGGCCTGGCA GCTCAGTCCG AGCCATTGTG GATGTGCACT TTGACCCTGC CACAACCTTT
    AGGGCGCCTG ATGTGGGCCA AGCCCTGCTC CGGCAGATAC AGGCATCCCG GCGCCGGTCC
    CTGGGCGTAC GGCGGCCACT GCAGGAGCAC GTGCGCTTTA TGGACTTTGA CTGGTTTCCT
    GCGTTCTTCA CGGGAGCCAC CACAGGAGCA ACACCCGCCG CAGCCACGGC CAGAGCCACC
    ACGGTGGCCC ACCTGCCACC TGCTGGGACC CAGTGGGCCC TCTACCCCAG TCACACAAGC
    CGGCCTGTGG GCAGGACCAC AGCTCCACCC ACCACACGCC AGCCTCCCAC CACTGCTCCC
    CGTCGCGTGC CCGGACGCCG GCCCTTGCCC CCAGGCACCC AGCAGCCCCC GAAGCCCTGC
    GACTCCCAGC CCTGCCTCCA TGGGGGAACC TGCCAGGACC AGGACTCGGG TGGAGGCTTC
    ACCTGCAGCT GCCCAGTGGG CAGGAGGGGT GCCGTCTGTG AGAAGGCCCT GTACCTCTCG
    GTGCCAGCTT TCAATGGCCG CTCCTTCCTG GCCTTCCCCA CCCTCCGTGC CTACCACACG
    CTGCGCCTAG CACTTGAGTT CCGGGCACTA GAGCCACAGG GCCTGCTGCT CTATAATGGC
    AATGCCCAGG GCAAGGACTT CCTGGCATTG ACGCTGCTGG GGGGCCGTGT GCAGCTCAGG
    TTTGACACAG GTTCCGGGCC TGCAGTACTG ACCAGCTCAG TGCCTATAGA GCCTGGCCGC
    TGGCATCGCT TGGAGCTGTC TCGGCACTGG CGCCGGGGAA CACTTTCAGT GGATGGTGAG
    CCCCTGGTGC TGGGCCACAG CCCCAGCGGT ACAGATGGTC TCAACCTGGA CACAGACCTT
    TTTGTGGGCG GCATCCCTGA GGATCAGGCT TCTGTGGTAC TCGAAAGGAC GTCCATCAGC
    GTTGGCCTCA AGGGCTGCAT CCGCCTGCTG GATGTCAATA ATCAGCGTTT AGAGCTCAGC
    AGCTGGCCAG GGGCTGCAGC CCGAAGCTCC GGGGTGGGCG AGTGTGGAGA CCACCCCTGC
    CTGCCCAACC CCTGCCTCGA CGGGGCTCCG TGCCAGGCCC TGGAGGCTGG CATGTTCCAC
    TGTCAGTGCC CACCTGGCCG CTTTGGTCCA ACCTGTAGTG GTGACAAGAA CCCCTGTGAG
    CCGAACCCCT GCCATGGGGC AGCCCCCTGC CGTGTGCTGC CTGATGGGGA GGCCAAGTGT
    GAATGCCCCC GAGGACGAGG GGGTGCCATC TGCCAGACAG TCTCGGAGCG GGATTCTTCC
    CAGCCCTTCC TGGCTGACTT CCATGGCTTC TCCTACCTGG AGCTGAAAGG GTTGCATACC
    TTTGAGCGGG ACCTGGGGGA GAAGCTGGCA CTGGAGGTGG TGTTCCTGGC CCGTGGGCCC
    AGTGGTTTGC TCCTCTACAA TGGGCAGAAA ACAGACGGCA AGGGGGACTT TGTGTCACTG
    GCGTTGCATG ACCGCTTCTT GGAATTCCGC TATGACCTGG GCAAGGGAGC AGCGGTCATC
    AGGAGCAAGG AGCCGGTGGC CCTGGACACC TGGACCAGGG TCTCACTAGA GCGCAATGGC
    CGCAAGGGTG CCATGCGTGT CAGTGATGGG CCTCGCGTGC TGGGAGAGTC CCCGAAATCC
    CGCAAGGTGC CACACACTGT CCTCAACCTG AAGGAGCCAC TCTATGTTGG AGGGGCCCCT
    GACTTCAGCA AACTGGCCCG GGCAGCTGCG GTATTCTCTG GCTTCGATGG CGCCATCCAA
    CTGGTCTCCT TGAAAGGCCA CCAGCTGCTG TCCCAGGAGC ACGTGGTGCG GTCAGTGGAT
    GTCTCATCTT TTGCCCACCA CCCTTGTACT CAGGCCTCGG GCCACCCATG CCTCAATGGG
    GCCTCCTGCC TCCCTCGAGA GGCCTCCTAT GAGTGCCTGT GCCCCGGGGG CTTCTCAGGG
    CTTCACTGTG AGAAGGGGCT GATTGAGAAG TCAGCAGGGG ATGTGGACGC CCTGGCCTTT
    GACGGACAGA CCTACGTCGA GTACCTCAAT GCCGTGACTG AGAGCGAACT GACCAATGAG
    ATCCCAGCTG AGAAGGCCCT GCAGAGCAAC CACTTTGAGC TGAGCCTGCG CACGGAAGCC
    ACTCAGGGGC TGGTGCTGTG GAGCGGCAAG GCCACGGAGC GGGCCGACTA TGTCGCACTG
    GCCATCGTGG ATGGGCGCCT GCAGCTGACC TATGACCTGG GCTCCCAGCC CGTCGTGCTG
    CGCTCCACCG TGCCGGTCAA CACCAACCGC TGGCTACGAG TCAGGGCACA TAGGGAACAG
    AGGGAAGGTT CCCTTCAGGT GGGCAATGAG GCTCCCGTGA CTGGCTCTTC CCCGCTGGGT
    GCCACACAGC TAGACACAGA CGGAACTCTA TGGCTGGGTG GCCTGGAGAA GCTGCCCATA
    GGCCAGGCCC TGCCCAAGGC CTATGGCACG GGCTTTGTGG GCTGCCTGCG GGATGTGGTG
    GTGGGGCAGC GCCCACTGCA CCTGCTGGAG GATGCTGTCA CCAAGCCAGA GCTCCGACCC
    TGTGCCGCCC CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06696
    Clone ID Related Accession (Same CDS sequence) XM_005620362.2 , XM_005620362.3
    Accession Version XM_005620362.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6195bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product agrin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005620362.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
    GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG
    GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC
    CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC
    ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA
    GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC
    CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG
    ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC
    TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC
    AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT
    CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC
    TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG
    CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC
    CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC
    CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG
    CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG
    GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC
    CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG
    TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT
    GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT
    GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG
    GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA
    TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT
    GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG
    GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG
    TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT
    GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC
    TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT
    GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC
    TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG
    GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG
    GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG
    GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT
    GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG
    CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG
    CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC
    ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT
    GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT
    GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC
    TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG
    CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT
    GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT
    GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGACT CTGTTCTCCT TGCAGAGACT
    GTTGCTCCTG GGGCCCGCCC AACATCTGTA CCCAGGGCCA CCTCAGGGCT CAGCACGAGC
    AAGGCGCTGC CACCCGCCCC CAGCACCCTC CCCCTGGCTC CCAGCAGCAC CCCTCATAGC
    CGGCCCACGC CTCGACCCTC ACCAGGATCT CGGACCACTG CCAGCATTCC CAGGACTGTC
    ACGAGGCCTG TGCTGACCAT GCCCCCCACA GCCCCCGCCA CGGCAGCCAA CCTCGCCACA
    TCTGCTTTTG GCGAGTCTGG CAGTGCTGAC GGGAGCGGCG ATGAGGGGCT GAGTGGTGAC
    CTGGAGGCCA GTGGGACTGG CTCAGGGGGA CTCGAGCCCC CTGAAGGTGG CAGTGCTGTG
    ACCCCTGAGC CACCCATGGA GAGGGCTTCT TGCTACAACT CGCCTTTGGG CTGCTGCTCG
    GATGGCAAGA CGCCCTCGCT GGACGCAGAG GGTTCCAACT GTCCTGCCAC CAAGGCGTTC
    CAGGGTGTGT TGGAGCTTGA GGGCGTGGAG GGGCAAGAGC TGTTCTACAC GCCGGAGATG
    GCTGACCCCA AGTCAGAGCT TTTTGGGGAG ACTGCTAGGA GCATCGAGAG CACCCTGGAT
    GACCTCTTCC GGAACTCAGA GGTTAAGAAG GACTTCCGAA GCGTCCGGCT GAGGGACTTG
    GGGCCTGGCA GCTCAGTCCG AGCCATTGTG GATGTGCACT TTGACCCTGC CACAACCTTT
    AGGGCGCCTG ATGTGGGCCA AGCCCTGCTC CGGCAGATAC AGGCATCCCG GCGCCGGTCC
    CTGGGCGTAC GGCGGCCACT GCAGGAGCAC GTGCGCTTTA TGGACTTTGA CTGGTTTCCT
    GCGTTCTTCA CGGGAGCCAC CACAGGAGCA ACACCCGCCG CAGCCACGGC CAGAGCCACC
    ACGGTGGCCC ACCTGCCACC TGCTGGGACC CAGTGGGCCC TCTACCCCAG TCACACAAGC
    CGGCCTGTGG GCAGGACCAC AGCTCCACCC ACCACACGCC AGCCTCCCAC CACTGCTCCC
    CGTCGCGTGC CCGGACGCCG GCCCTTGCCC CCAGGCACCC AGCAGCCCCC GAAGCCCTGC
    GACTCCCAGC CCTGCCTCCA TGGGGGAACC TGCCAGGACC AGGACTCGGG TGGAGGCTTC
    ACCTGCAGCT GCCCAGTGGG CAGGAGGGGT GCCGTCTGTG AGAAGGCCCT GTACCTCTCG
    GTGCCAGCTT TCAATGGCCG CTCCTTCCTG GCCTTCCCCA CCCTCCGTGC CTACCACACG
    CTGCGCCTAG CACTTGAGTT CCGGGCACTA GAGCCACAGG GCCTGCTGCT CTATAATGGC
    AATGCCCAGG GCAAGGACTT CCTGGCATTG ACGCTGCTGG GGGGCCGTGT GCAGCTCAGG
    TTTGACACAG GTTCCGGGCC TGCAGTACTG ACCAGCTCAG TGCCTATAGA GCCTGGCCGC
    TGGCATCGCT TGGAGCTGTC TCGGCACTGG CGCCGGGGAA CACTTTCAGT GGATGGTGAG
    CCCCTGGTGC TGGGCCACAG CCCCAGCGGT ACAGATGGTC TCAACCTGGA CACAGACCTT
    TTTGTGGGCG GCATCCCTGA GGATCAGGCT TCTGTGGTAC TCGAAAGGAC GTCCATCAGC
    GTTGGCCTCA AGGGCTGCAT CCGCCTGCTG GATGTCAATA ATCAGCGTTT AGAGCTCAGC
    AGCTGGCCAG GGGCTGCAGC CCGAAGCTCC GGGGTGGGCG AGTGTGGAGA CCACCCCTGC
    CTGCCCAACC CCTGCCTCGA CGGGGCTCCG TGCCAGGCCC TGGAGGCTGG CATGTTCCAC
    TGTCAGTGCC CACCTGGCCG CTTTGGTCCA ACCTGTAGTG GTGACAAGAA CCCCTGTGAG
    CCGAACCCCT GCCATGGGGC AGCCCCCTGC CGTGTGCTGC CTGATGGGGA GGCCAAGTGT
    GAATGCCCCC GAGGACGAGG GGGTGCCATC TGCCAGACAG TCTCGGAGCG GGATTCTTCC
    CAGCCCTTCC TGGCTGACTT CCATGGCTTC TCCTACCTGG AGCTGAAAGG GTTGCATACC
    TTTGAGCGGG ACCTGGGGGA GAAGCTGGCA CTGGAGGTGG TGTTCCTGGC CCGTGGGCCC
    AGTGGTTTGC TCCTCTACAA TGGGCAGAAA ACAGACGGCA AGGGGGACTT TGTGTCACTG
    GCGTTGCATG ACCGCTTCTT GGAATTCCGC TATGACCTGG GCAAGGGAGC AGCGGTCATC
    AGGAGCAAGG AGCCGGTGGC CCTGGACACC TGGACCAGGG TCTCACTAGA GCGCAATGGC
    CGCAAGGGTG CCATGCGTGT CAGTGATGGG CCTCGCGTGC TGGGAGAGTC CCCGAAATCC
    CGCAAGGTGC CACACACTGT CCTCAACCTG AAGGAGCCAC TCTATGTTGG AGGGGCCCCT
    GACTTCAGCA AACTGGCCCG GGCAGCTGCG GTATTCTCTG GCTTCGATGG CGCCATCCAA
    CTGGTCTCCT TGAAAGGCCA CCAGCTGCTG TCCCAGGAGC ACGTGGTGCG GTCAGTGGAT
    GTCTCATCTT TTGCCCACCA CCCTTGTACT CAGGCCTCGG GCCACCCATG CCTCAATGGG
    GCCTCCTGCC TCCCTCGAGA GGCCTCCTAT GAGTGCCTGT GCCCCGGGGG CTTCTCAGGG
    CTTCACTGTG AGAAGGGGCT GATTGAGAAG TCAGCAGGGG ATGTGGACGC CCTGGCCTTT
    GACGGACAGA CCTACGTCGA GTACCTCAAT GCCGTGACTG AGAGCGAACT GACCAATGAG
    ATCCCAGCTG AGAAGGCCCT GCAGAGCAAC CACTTTGAGC TGAGCCTGCG CACGGAAGCC
    ACTCAGGGGC TGGTGCTGTG GAGCGGCAAG GCCACGGAGC GGGCCGACTA TGTCGCACTG
    GCCATCGTGG ATGGGCGCCT GCAGCTGACC TATGACCTGG GCTCCCAGCC CGTCGTGCTG
    CGCTCCACCG TGCCGGTCAA CACCAACCGC TGGCTACGAG TCAGGGCACA TAGGGAACAG
    AGGGAAGGTT CCCTTCAGGT GGGCAATGAG GCTCCCGTGA CTGGCTCTTC CCCGCTGGGT
    GCCACACAGC TAGACACAGA CGGAACTCTA TGGCTGGGTG GCCTGGAGAA GCTGCCCATA
    GGCCAGGCCC TGCCCAAGGC CTATGGCACG GGCTTTGTGG GCTGCCTGCG GGATGTGGTG
    GTGGGGCAGC GCCCACTGCA CCTGCTGGAG GATGCTGTCA CCAAGCCAGA GCTCCGACCC
    TGTGCCGCCC CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005620419.1
    CDS81..6275
    Translation

    Target ORF information:

    RefSeq Version XM_005620362.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris agrin (AGRN), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005620362.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
    GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG
    GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC
    CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC
    ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA
    GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC
    CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG
    ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC
    TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC
    AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT
    CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC
    TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG
    CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC
    CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC
    CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG
    CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG
    GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC
    CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG
    TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT
    GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT
    GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG
    GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA
    TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT
    GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG
    GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG
    TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT
    GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC
    TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT
    GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC
    TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG
    GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG
    GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG
    GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT
    GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG
    CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG
    CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC
    ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT
    GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT
    GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC
    TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG
    CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT
    GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT
    GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGACT CTGTTCTCCT TGCAGAGACT
    GTTGCTCCTG GGGCCCGCCC AACATCTGTA CCCAGGGCCA CCTCAGGGCT CAGCACGAGC
    AAGGCGCTGC CACCCGCCCC CAGCACCCTC CCCCTGGCTC CCAGCAGCAC CCCTCATAGC
    CGGCCCACGC CTCGACCCTC ACCAGGATCT CGGACCACTG CCAGCATTCC CAGGACTGTC
    ACGAGGCCTG TGCTGACCAT GCCCCCCACA GCCCCCGCCA CGGCAGCCAA CCTCGCCACA
    TCTGCTTTTG GCGAGTCTGG CAGTGCTGAC GGGAGCGGCG ATGAGGGGCT GAGTGGTGAC
    CTGGAGGCCA GTGGGACTGG CTCAGGGGGA CTCGAGCCCC CTGAAGGTGG CAGTGCTGTG
    ACCCCTGAGC CACCCATGGA GAGGGCTTCT TGCTACAACT CGCCTTTGGG CTGCTGCTCG
    GATGGCAAGA CGCCCTCGCT GGACGCAGAG GGTTCCAACT GTCCTGCCAC CAAGGCGTTC
    CAGGGTGTGT TGGAGCTTGA GGGCGTGGAG GGGCAAGAGC TGTTCTACAC GCCGGAGATG
    GCTGACCCCA AGTCAGAGCT TTTTGGGGAG ACTGCTAGGA GCATCGAGAG CACCCTGGAT
    GACCTCTTCC GGAACTCAGA GGTTAAGAAG GACTTCCGAA GCGTCCGGCT GAGGGACTTG
    GGGCCTGGCA GCTCAGTCCG AGCCATTGTG GATGTGCACT TTGACCCTGC CACAACCTTT
    AGGGCGCCTG ATGTGGGCCA AGCCCTGCTC CGGCAGATAC AGGCATCCCG GCGCCGGTCC
    CTGGGCGTAC GGCGGCCACT GCAGGAGCAC GTGCGCTTTA TGGACTTTGA CTGGTTTCCT
    GCGTTCTTCA CGGGAGCCAC CACAGGAGCA ACACCCGCCG CAGCCACGGC CAGAGCCACC
    ACGGTGGCCC ACCTGCCACC TGCTGGGACC CAGTGGGCCC TCTACCCCAG TCACACAAGC
    CGGCCTGTGG GCAGGACCAC AGCTCCACCC ACCACACGCC AGCCTCCCAC CACTGCTCCC
    CGTCGCGTGC CCGGACGCCG GCCCTTGCCC CCAGGCACCC AGCAGCCCCC GAAGCCCTGC
    GACTCCCAGC CCTGCCTCCA TGGGGGAACC TGCCAGGACC AGGACTCGGG TGGAGGCTTC
    ACCTGCAGCT GCCCAGTGGG CAGGAGGGGT GCCGTCTGTG AGAAGGCCCT GTACCTCTCG
    GTGCCAGCTT TCAATGGCCG CTCCTTCCTG GCCTTCCCCA CCCTCCGTGC CTACCACACG
    CTGCGCCTAG CACTTGAGTT CCGGGCACTA GAGCCACAGG GCCTGCTGCT CTATAATGGC
    AATGCCCAGG GCAAGGACTT CCTGGCATTG ACGCTGCTGG GGGGCCGTGT GCAGCTCAGG
    TTTGACACAG GTTCCGGGCC TGCAGTACTG ACCAGCTCAG TGCCTATAGA GCCTGGCCGC
    TGGCATCGCT TGGAGCTGTC TCGGCACTGG CGCCGGGGAA CACTTTCAGT GGATGGTGAG
    CCCCTGGTGC TGGGCCACAG CCCCAGCGGT ACAGATGGTC TCAACCTGGA CACAGACCTT
    TTTGTGGGCG GCATCCCTGA GGATCAGGCT TCTGTGGTAC TCGAAAGGAC GTCCATCAGC
    GTTGGCCTCA AGGGCTGCAT CCGCCTGCTG GATGTCAATA ATCAGCGTTT AGAGCTCAGC
    AGCTGGCCAG GGGCTGCAGC CCGAAGCTCC GGGGTGGGCG AGTGTGGAGA CCACCCCTGC
    CTGCCCAACC CCTGCCTCGA CGGGGCTCCG TGCCAGGCCC TGGAGGCTGG CATGTTCCAC
    TGTCAGTGCC CACCTGGCCG CTTTGGTCCA ACCTGTAGTG GTGACAAGAA CCCCTGTGAG
    CCGAACCCCT GCCATGGGGC AGCCCCCTGC CGTGTGCTGC CTGATGGGGA GGCCAAGTGT
    GAATGCCCCC GAGGACGAGG GGGTGCCATC TGCCAGACAG TCTCGGAGCG GGATTCTTCC
    CAGCCCTTCC TGGCTGACTT CCATGGCTTC TCCTACCTGG AGCTGAAAGG GTTGCATACC
    TTTGAGCGGG ACCTGGGGGA GAAGCTGGCA CTGGAGGTGG TGTTCCTGGC CCGTGGGCCC
    AGTGGTTTGC TCCTCTACAA TGGGCAGAAA ACAGACGGCA AGGGGGACTT TGTGTCACTG
    GCGTTGCATG ACCGCTTCTT GGAATTCCGC TATGACCTGG GCAAGGGAGC AGCGGTCATC
    AGGAGCAAGG AGCCGGTGGC CCTGGACACC TGGACCAGGG TCTCACTAGA GCGCAATGGC
    CGCAAGGGTG CCATGCGTGT CAGTGATGGG CCTCGCGTGC TGGGAGAGTC CCCGAAATCC
    CGCAAGGTGC CACACACTGT CCTCAACCTG AAGGAGCCAC TCTATGTTGG AGGGGCCCCT
    GACTTCAGCA AACTGGCCCG GGCAGCTGCG GTATTCTCTG GCTTCGATGG CGCCATCCAA
    CTGGTCTCCT TGAAAGGCCA CCAGCTGCTG TCCCAGGAGC ACGTGGTGCG GTCAGTGGAT
    GTCTCATCTT TTGCCCACCA CCCTTGTACT CAGGCCTCGG GCCACCCATG CCTCAATGGG
    GCCTCCTGCC TCCCTCGAGA GGCCTCCTAT GAGTGCCTGT GCCCCGGGGG CTTCTCAGGG
    CTTCACTGTG AGAAGGGGCT GATTGAGAAG TCAGCAGGGG ATGTGGACGC CCTGGCCTTT
    GACGGACAGA CCTACGTCGA GTACCTCAAT GCCGTGACTG AGAGCGAACT GACCAATGAG
    ATCCCAGCTG AGAAGGCCCT GCAGAGCAAC CACTTTGAGC TGAGCCTGCG CACGGAAGCC
    ACTCAGGGGC TGGTGCTGTG GAGCGGCAAG GCCACGGAGC GGGCCGACTA TGTCGCACTG
    GCCATCGTGG ATGGGCGCCT GCAGCTGACC TATGACCTGG GCTCCCAGCC CGTCGTGCTG
    CGCTCCACCG TGCCGGTCAA CACCAACCGC TGGCTACGAG TCAGGGCACA TAGGGAACAG
    AGGGAAGGTT CCCTTCAGGT GGGCAATGAG GCTCCCGTGA CTGGCTCTTC CCCGCTGGGT
    GCCACACAGC TAGACACAGA CGGAACTCTA TGGCTGGGTG GCCTGGAGAA GCTGCCCATA
    GGCCAGGCCC TGCCCAAGGC CTATGGCACG GGCTTTGTGG GCTGCCTGCG GGATGTGGTG
    GTGGGGCAGC GCCCACTGCA CCTGCTGGAG GATGCTGTCA CCAAGCCAGA GCTCCGACCC
    TGTGCCGCCC CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06697
    Clone ID Related Accession (Same CDS sequence) XM_005620363.2 , XM_005620363.1
    Accession Version XM_005620363.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6171bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product agrin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005620363.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
    GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG
    GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC
    CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC
    ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA
    GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC
    CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG
    ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC
    TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC
    AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT
    CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC
    TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG
    CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC
    CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC
    CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG
    CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG
    GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC
    CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG
    TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT
    GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT
    GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG
    GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA
    TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT
    GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG
    GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG
    TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT
    GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC
    TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT
    GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC
    TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG
    GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG
    GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG
    GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT
    GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG
    CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG
    CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC
    ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT
    GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT
    GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC
    TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG
    CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT
    GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT
    GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGACT CTGTTCTCCT TGCAGAGACT
    GTTGCTCCTG GGGCCCGCCC AACATCTGTA CCCAGGGCCA CCTCAGGGCT CAGCACGAGC
    AAGGCGCTGC CACCCGCCCC CAGCACCCTC CCCCTGGCTC CCAGCAGCAC CCCTCATAGC
    CGGCCCACGC CTCGACCCTC ACCAGGATCT CGGACCACTG CCAGCATTCC CAGGACTGTC
    ACGAGGCCTG TGCTGACCAT GCCCCCCACA GCCCCCGCCA CGGCAGCCAA CCTCGCCACA
    TCTGCTTTTG GCGAGTCTGG CAGTGCTGAC GGGAGCGGCG ATGAGGGGCT GAGTGGTGAC
    CTGGAGGCCA GTGGGACTGG CTCAGGGGGA CTCGAGCCCC CTGAAGGTGG CAGTGCTGTG
    ACCCCTGAGC CACCCATGGA GAGGGCTTCT TGCTACAACT CGCCTTTGGG CTGCTGCTCG
    GATGGCAAGA CGCCCTCGCT GGACGCAGAG GGTTCCAACT GTCCTGCCAC CAAGGCGTTC
    CAGGGTGTGT TGGAGCTTGA GGGCGTGGAG GGGCAAGAGC TGTTCTACAC GCCGGAGATG
    GCTGACCCCA AGTCAGAGCT TTTTGGGGAG ACTGCTAGGA GCATCGAGAG CACCCTGGAT
    GACCTCTTCC GGAACTCAGA GGTTAAGAAG GACTTCCGAA GCGTCCGGCT GAGGGACTTG
    GGGCCTGGCA GCTCAGTCCG AGCCATTGTG GATGTGCACT TTGACCCTGC CACAACCTTT
    AGGGCGCCTG ATGTGGGCCA AGCCCTGCTC CGGCAGATAC AGGCATCCCG GCGCCGGTCC
    CTGGGCGTAC GGCGGCCACT GCAGGAGCAC GTGCGCTTTA TGGACTTTGA CTGGTTTCCT
    GCGTTCTTCA CGGGAGCCAC CACAGGAGCA ACACCCGCCG CAGCCACGGC CAGAGCCACC
    ACGGTGGCCC ACCTGCCACC TGCTGGGACC CAGTGGGCCC TCTACCCCAG TCACACAAGC
    CGGCCTGTGG GCAGGACCAC AGCTCCACCC ACCACACGCC AGCCTCCCAC CACTGCTCCC
    CGTCGCGTGC CCGGACGCCG GCCCTTGCCC CCAGGCACCC AGCAGCCCCC GAAGCCCTGC
    GACTCCCAGC CCTGCCTCCA TGGGGGAACC TGCCAGGACC AGGACTCGGG TGGAGGCTTC
    ACCTGCAGCT GCCCAGTGGG CAGGAGGGGT GCCGTCTGTG AGAAGGCCCT GTACCTCTCG
    GTGCCAGCTT TCAATGGCCG CTCCTTCCTG GCCTTCCCCA CCCTCCGTGC CTACCACACG
    CTGCGCCTAG CACTTGAGTT CCGGGCACTA GAGCCACAGG GCCTGCTGCT CTATAATGGC
    AATGCCCAGG GCAAGGACTT CCTGGCATTG ACGCTGCTGG GGGGCCGTGT GCAGCTCAGG
    TTTGACACAG GTTCCGGGCC TGCAGTACTG ACCAGCTCAG TGCCTATAGA GCCTGGCCGC
    TGGCATCGCT TGGAGCTGTC TCGGCACTGG CGCCGGGGAA CACTTTCAGT GGATGGTGAG
    CCCCTGGTGC TGGGCCACAG CCCCAGCGGT ACAGATGGTC TCAACCTGGA CACAGACCTT
    TTTGTGGGCG GCATCCCTGA GGATCAGGCT TCTGTGGTAC TCGAAAGGAC GTCCATCAGC
    GTTGGCCTCA AGGGCTGCAT CCGCCTGCTG GATGTCAATA ATCAGCGTTT AGAGCTCAGC
    AGCTGGCCAG GGGCTGCAGC CCGAAGCTCC GGGGTGGGCG AGTGTGGAGA CCACCCCTGC
    CTGCCCAACC CCTGCCTCGA CGGGGCTCCG TGCCAGGCCC TGGAGGCTGG CATGTTCCAC
    TGTCAGTGCC CACCTGGCCG CTTTGGTCCA ACCTGTAGTG GTGACAAGAA CCCCTGTGAG
    CCGAACCCCT GCCATGGGGC AGCCCCCTGC CGTGTGCTGC CTGATGGGGA GGCCAAGTGT
    GAATGCCCCC GAGGACGAGG GGGTGCCATC TGCCAGACAG TCTCGGAGCG GGATTCTTCC
    CAGCCCTTCC TGGCTGACTT CCATGGCTTC TCCTACCTGG AGCTGAAAGG GTTGCATACC
    TTTGAGCGGG ACCTGGGGGA GAAGCTGGCA CTGGAGGTGG TGTTCCTGGC CCGTGGGCCC
    AGTGGTTTGC TCCTCTACAA TGGGCAGAAA ACAGACGGCA AGGGGGACTT TGTGTCACTG
    GCGTTGCATG ACCGCTTCTT GGAATTCCGC TATGACCTGG GCAAGGGAGC AGCGGTCATC
    AGGAGCAAGG AGCCGGTGGC CCTGGACACC TGGACCAGGG TCTCACTAGA GCGCAATGGC
    CGCAAGGGTG CCATGCGTGT CAGTGATGGG CCTCGCGTGC TGGGAGAGTC CCCGAAATCC
    CGCAAGGTGC CACACACTGT CCTCAACCTG AAGGAGCCAC TCTATGTTGG AGGGGCCCCT
    GACTTCAGCA AACTGGCCCG GGCAGCTGCG GTATTCTCTG GCTTCGATGG CGCCATCCAA
    CTGGTCTCCT TGAAAGGCCA CCAGCTGCTG TCCCAGGAGC ACGTGGTGCG GTCAGTGGAT
    GTCTCATCTT TTGCCCACCA CCCTTGTACT CAGGCCTCGG GCCACCCATG CCTCAATGGG
    GCCTCCTGCC TCCCTCGAGA GGCCTCCTAT GAGTGCCTGT GCCCCGGGGG CTTCTCAGGG
    CTTCACTGTG AGAAGGGGCT GATTGAGAAG TCAGCAGGGG ATGTGGACGC CCTGGCCTTT
    GACGGACAGA CCTACGTCGA GTACCTCAAT GCCGTGACTG AGAGTGAGAA GGCCCTGCAG
    AGCAACCACT TTGAGCTGAG CCTGCGCACG GAAGCCACTC AGGGGCTGGT GCTGTGGAGC
    GGCAAGGCCA CGGAGCGGGC CGACTATGTC GCACTGGCCA TCGTGGATGG GCGCCTGCAG
    CTGACCTATG ACCTGGGCTC CCAGCCCGTC GTGCTGCGCT CCACCGTGCC GGTCAACACC
    AACCGCTGGC TACGAGTCAG GGCACATAGG GAACAGAGGG AAGGTTCCCT TCAGGTGGGC
    AATGAGGCTC CCGTGACTGG CTCTTCCCCG CTGGGTGCCA CACAGCTAGA CACAGACGGA
    ACTCTATGGC TGGGTGGCCT GGAGAAGCTG CCCATAGGCC AGGCCCTGCC CAAGGCCTAT
    GGCACGGGCT TTGTGGGCTG CCTGCGGGAT GTGGTGGTGG GGCAGCGCCC ACTGCACCTG
    CTGGAGGATG CTGTCACCAA GCCAGAGCTC CGACCCTGTG CCGCCCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005620420.1
    CDS80..6250
    Translation

    Target ORF information:

    RefSeq Version XM_005620363.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris agrin (AGRN), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005620363.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
    GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG
    GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC
    CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC
    ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA
    GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC
    CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG
    ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC
    TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC
    AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT
    CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC
    TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG
    CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC
    CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC
    CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG
    CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG
    GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC
    CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG
    TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT
    GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT
    GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG
    GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA
    TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT
    GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG
    GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG
    TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT
    GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC
    TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT
    GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC
    TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG
    GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG
    GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG
    GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT
    GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG
    CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG
    CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC
    ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT
    GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT
    GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC
    TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG
    CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT
    GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT
    GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGACT CTGTTCTCCT TGCAGAGACT
    GTTGCTCCTG GGGCCCGCCC AACATCTGTA CCCAGGGCCA CCTCAGGGCT CAGCACGAGC
    AAGGCGCTGC CACCCGCCCC CAGCACCCTC CCCCTGGCTC CCAGCAGCAC CCCTCATAGC
    CGGCCCACGC CTCGACCCTC ACCAGGATCT CGGACCACTG CCAGCATTCC CAGGACTGTC
    ACGAGGCCTG TGCTGACCAT GCCCCCCACA GCCCCCGCCA CGGCAGCCAA CCTCGCCACA
    TCTGCTTTTG GCGAGTCTGG CAGTGCTGAC GGGAGCGGCG ATGAGGGGCT GAGTGGTGAC
    CTGGAGGCCA GTGGGACTGG CTCAGGGGGA CTCGAGCCCC CTGAAGGTGG CAGTGCTGTG
    ACCCCTGAGC CACCCATGGA GAGGGCTTCT TGCTACAACT CGCCTTTGGG CTGCTGCTCG
    GATGGCAAGA CGCCCTCGCT GGACGCAGAG GGTTCCAACT GTCCTGCCAC CAAGGCGTTC
    CAGGGTGTGT TGGAGCTTGA GGGCGTGGAG GGGCAAGAGC TGTTCTACAC GCCGGAGATG
    GCTGACCCCA AGTCAGAGCT TTTTGGGGAG ACTGCTAGGA GCATCGAGAG CACCCTGGAT
    GACCTCTTCC GGAACTCAGA GGTTAAGAAG GACTTCCGAA GCGTCCGGCT GAGGGACTTG
    GGGCCTGGCA GCTCAGTCCG AGCCATTGTG GATGTGCACT TTGACCCTGC CACAACCTTT
    AGGGCGCCTG ATGTGGGCCA AGCCCTGCTC CGGCAGATAC AGGCATCCCG GCGCCGGTCC
    CTGGGCGTAC GGCGGCCACT GCAGGAGCAC GTGCGCTTTA TGGACTTTGA CTGGTTTCCT
    GCGTTCTTCA CGGGAGCCAC CACAGGAGCA ACACCCGCCG CAGCCACGGC CAGAGCCACC
    ACGGTGGCCC ACCTGCCACC TGCTGGGACC CAGTGGGCCC TCTACCCCAG TCACACAAGC
    CGGCCTGTGG GCAGGACCAC AGCTCCACCC ACCACACGCC AGCCTCCCAC CACTGCTCCC
    CGTCGCGTGC CCGGACGCCG GCCCTTGCCC CCAGGCACCC AGCAGCCCCC GAAGCCCTGC
    GACTCCCAGC CCTGCCTCCA TGGGGGAACC TGCCAGGACC AGGACTCGGG TGGAGGCTTC
    ACCTGCAGCT GCCCAGTGGG CAGGAGGGGT GCCGTCTGTG AGAAGGCCCT GTACCTCTCG
    GTGCCAGCTT TCAATGGCCG CTCCTTCCTG GCCTTCCCCA CCCTCCGTGC CTACCACACG
    CTGCGCCTAG CACTTGAGTT CCGGGCACTA GAGCCACAGG GCCTGCTGCT CTATAATGGC
    AATGCCCAGG GCAAGGACTT CCTGGCATTG ACGCTGCTGG GGGGCCGTGT GCAGCTCAGG
    TTTGACACAG GTTCCGGGCC TGCAGTACTG ACCAGCTCAG TGCCTATAGA GCCTGGCCGC
    TGGCATCGCT TGGAGCTGTC TCGGCACTGG CGCCGGGGAA CACTTTCAGT GGATGGTGAG
    CCCCTGGTGC TGGGCCACAG CCCCAGCGGT ACAGATGGTC TCAACCTGGA CACAGACCTT
    TTTGTGGGCG GCATCCCTGA GGATCAGGCT TCTGTGGTAC TCGAAAGGAC GTCCATCAGC
    GTTGGCCTCA AGGGCTGCAT CCGCCTGCTG GATGTCAATA ATCAGCGTTT AGAGCTCAGC
    AGCTGGCCAG GGGCTGCAGC CCGAAGCTCC GGGGTGGGCG AGTGTGGAGA CCACCCCTGC
    CTGCCCAACC CCTGCCTCGA CGGGGCTCCG TGCCAGGCCC TGGAGGCTGG CATGTTCCAC
    TGTCAGTGCC CACCTGGCCG CTTTGGTCCA ACCTGTAGTG GTGACAAGAA CCCCTGTGAG
    CCGAACCCCT GCCATGGGGC AGCCCCCTGC CGTGTGCTGC CTGATGGGGA GGCCAAGTGT
    GAATGCCCCC GAGGACGAGG GGGTGCCATC TGCCAGACAG TCTCGGAGCG GGATTCTTCC
    CAGCCCTTCC TGGCTGACTT CCATGGCTTC TCCTACCTGG AGCTGAAAGG GTTGCATACC
    TTTGAGCGGG ACCTGGGGGA GAAGCTGGCA CTGGAGGTGG TGTTCCTGGC CCGTGGGCCC
    AGTGGTTTGC TCCTCTACAA TGGGCAGAAA ACAGACGGCA AGGGGGACTT TGTGTCACTG
    GCGTTGCATG ACCGCTTCTT GGAATTCCGC TATGACCTGG GCAAGGGAGC AGCGGTCATC
    AGGAGCAAGG AGCCGGTGGC CCTGGACACC TGGACCAGGG TCTCACTAGA GCGCAATGGC
    CGCAAGGGTG CCATGCGTGT CAGTGATGGG CCTCGCGTGC TGGGAGAGTC CCCGAAATCC
    CGCAAGGTGC CACACACTGT CCTCAACCTG AAGGAGCCAC TCTATGTTGG AGGGGCCCCT
    GACTTCAGCA AACTGGCCCG GGCAGCTGCG GTATTCTCTG GCTTCGATGG CGCCATCCAA
    CTGGTCTCCT TGAAAGGCCA CCAGCTGCTG TCCCAGGAGC ACGTGGTGCG GTCAGTGGAT
    GTCTCATCTT TTGCCCACCA CCCTTGTACT CAGGCCTCGG GCCACCCATG CCTCAATGGG
    GCCTCCTGCC TCCCTCGAGA GGCCTCCTAT GAGTGCCTGT GCCCCGGGGG CTTCTCAGGG
    CTTCACTGTG AGAAGGGGCT GATTGAGAAG TCAGCAGGGG ATGTGGACGC CCTGGCCTTT
    GACGGACAGA CCTACGTCGA GTACCTCAAT GCCGTGACTG AGAGTGAGAA GGCCCTGCAG
    AGCAACCACT TTGAGCTGAG CCTGCGCACG GAAGCCACTC AGGGGCTGGT GCTGTGGAGC
    GGCAAGGCCA CGGAGCGGGC CGACTATGTC GCACTGGCCA TCGTGGATGG GCGCCTGCAG
    CTGACCTATG ACCTGGGCTC CCAGCCCGTC GTGCTGCGCT CCACCGTGCC GGTCAACACC
    AACCGCTGGC TACGAGTCAG GGCACATAGG GAACAGAGGG AAGGTTCCCT TCAGGTGGGC
    AATGAGGCTC CCGTGACTGG CTCTTCCCCG CTGGGTGCCA CACAGCTAGA CACAGACGGA
    ACTCTATGGC TGGGTGGCCT GGAGAAGCTG CCCATAGGCC AGGCCCTGCC CAAGGCCTAT
    GGCACGGGCT TTGTGGGCTG CCTGCGGGAT GTGGTGGTGG GGCAGCGCCC ACTGCACCTG
    CTGGAGGATG CTGTCACCAA GCCAGAGCTC CGACCCTGTG CCGCCCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06697
    Clone ID Related Accession (Same CDS sequence) XM_005620363.2 , XM_005620363.1
    Accession Version XM_005620363.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6171bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product agrin isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726058.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
    GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG
    GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC
    CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC
    ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA
    GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC
    CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG
    ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC
    TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC
    AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT
    CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC
    TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG
    CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC
    CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC
    CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG
    CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG
    GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC
    CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG
    TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT
    GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT
    GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG
    GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA
    TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT
    GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG
    GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG
    TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT
    GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC
    TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT
    GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC
    TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG
    GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG
    GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG
    GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT
    GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG
    CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG
    CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC
    ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT
    GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT
    GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC
    TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG
    CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT
    GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT
    GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGACT CTGTTCTCCT TGCAGAGACT
    GTTGCTCCTG GGGCCCGCCC AACATCTGTA CCCAGGGCCA CCTCAGGGCT CAGCACGAGC
    AAGGCGCTGC CACCCGCCCC CAGCACCCTC CCCCTGGCTC CCAGCAGCAC CCCTCATAGC
    CGGCCCACGC CTCGACCCTC ACCAGGATCT CGGACCACTG CCAGCATTCC CAGGACTGTC
    ACGAGGCCTG TGCTGACCAT GCCCCCCACA GCCCCCGCCA CGGCAGCCAA CCTCGCCACA
    TCTGCTTTTG GCGAGTCTGG CAGTGCTGAC GGGAGCGGCG ATGAGGGGCT GAGTGGTGAC
    CTGGAGGCCA GTGGGACTGG CTCAGGGGGA CTCGAGCCCC CTGAAGGTGG CAGTGCTGTG
    ACCCCTGAGC CACCCATGGA GAGGGCTTCT TGCTACAACT CGCCTTTGGG CTGCTGCTCG
    GATGGCAAGA CGCCCTCGCT GGACGCAGAG GGTTCCAACT GTCCTGCCAC CAAGGCGTTC
    CAGGGTGTGT TGGAGCTTGA GGGCGTGGAG GGGCAAGAGC TGTTCTACAC GCCGGAGATG
    GCTGACCCCA AGTCAGAGCT TTTTGGGGAG ACTGCTAGGA GCATCGAGAG CACCCTGGAT
    GACCTCTTCC GGAACTCAGA GGTTAAGAAG GACTTCCGAA GCGTCCGGCT GAGGGACTTG
    GGGCCTGGCA GCTCAGTCCG AGCCATTGTG GATGTGCACT TTGACCCTGC CACAACCTTT
    AGGGCGCCTG ATGTGGGCCA AGCCCTGCTC CGGCAGATAC AGGCATCCCG GCGCCGGTCC
    CTGGGCGTAC GGCGGCCACT GCAGGAGCAC GTGCGCTTTA TGGACTTTGA CTGGTTTCCT
    GCGTTCTTCA CGGGAGCCAC CACAGGAGCA ACACCCGCCG CAGCCACGGC CAGAGCCACC
    ACGGTGGCCC ACCTGCCACC TGCTGGGACC CAGTGGGCCC TCTACCCCAG TCACACAAGC
    CGGCCTGTGG GCAGGACCAC AGCTCCACCC ACCACACGCC AGCCTCCCAC CACTGCTCCC
    CGTCGCGTGC CCGGACGCCG GCCCTTGCCC CCAGGCACCC AGCAGCCCCC GAAGCCCTGC
    GACTCCCAGC CCTGCCTCCA TGGGGGAACC TGCCAGGACC AGGACTCGGG TGGAGGCTTC
    ACCTGCAGCT GCCCAGTGGG CAGGAGGGGT GCCGTCTGTG AGAAGGCCCT GTACCTCTCG
    GTGCCAGCTT TCAATGGCCG CTCCTTCCTG GCCTTCCCCA CCCTCCGTGC CTACCACACG
    CTGCGCCTAG CACTTGAGTT CCGGGCACTA GAGCCACAGG GCCTGCTGCT CTATAATGGC
    AATGCCCAGG GCAAGGACTT CCTGGCATTG ACGCTGCTGG GGGGCCGTGT GCAGCTCAGG
    TTTGACACAG GTTCCGGGCC TGCAGTACTG ACCAGCTCAG TGCCTATAGA GCCTGGCCGC
    TGGCATCGCT TGGAGCTGTC TCGGCACTGG CGCCGGGGAA CACTTTCAGT GGATGGTGAG
    CCCCTGGTGC TGGGCCACAG CCCCAGCGGT ACAGATGGTC TCAACCTGGA CACAGACCTT
    TTTGTGGGCG GCATCCCTGA GGATCAGGCT TCTGTGGTAC TCGAAAGGAC GTCCATCAGC
    GTTGGCCTCA AGGGCTGCAT CCGCCTGCTG GATGTCAATA ATCAGCGTTT AGAGCTCAGC
    AGCTGGCCAG GGGCTGCAGC CCGAAGCTCC GGGGTGGGCG AGTGTGGAGA CCACCCCTGC
    CTGCCCAACC CCTGCCTCGA CGGGGCTCCG TGCCAGGCCC TGGAGGCTGG CATGTTCCAC
    TGTCAGTGCC CACCTGGCCG CTTTGGTCCA ACCTGTAGTG GTGACAAGAA CCCCTGTGAG
    CCGAACCCCT GCCATGGGGC AGCCCCCTGC CGTGTGCTGC CTGATGGGGA GGCCAAGTGT
    GAATGCCCCC GAGGACGAGG GGGTGCCATC TGCCAGACAG TCTCGGAGCG GGATTCTTCC
    CAGCCCTTCC TGGCTGACTT CCATGGCTTC TCCTACCTGG AGCTGAAAGG GTTGCATACC
    TTTGAGCGGG ACCTGGGGGA GAAGCTGGCA CTGGAGGTGG TGTTCCTGGC CCGTGGGCCC
    AGTGGTTTGC TCCTCTACAA TGGGCAGAAA ACAGACGGCA AGGGGGACTT TGTGTCACTG
    GCGTTGCATG ACCGCTTCTT GGAATTCCGC TATGACCTGG GCAAGGGAGC AGCGGTCATC
    AGGAGCAAGG AGCCGGTGGC CCTGGACACC TGGACCAGGG TCTCACTAGA GCGCAATGGC
    CGCAAGGGTG CCATGCGTGT CAGTGATGGG CCTCGCGTGC TGGGAGAGTC CCCGAAATCC
    CGCAAGGTGC CACACACTGT CCTCAACCTG AAGGAGCCAC TCTATGTTGG AGGGGCCCCT
    GACTTCAGCA AACTGGCCCG GGCAGCTGCG GTATTCTCTG GCTTCGATGG CGCCATCCAA
    CTGGTCTCCT TGAAAGGCCA CCAGCTGCTG TCCCAGGAGC ACGTGGTGCG GTCAGTGGAT
    GTCTCATCTT TTGCCCACCA CCCTTGTACT CAGGCCTCGG GCCACCCATG CCTCAATGGG
    GCCTCCTGCC TCCCTCGAGA GGCCTCCTAT GAGTGCCTGT GCCCCGGGGG CTTCTCAGGG
    CTTCACTGTG AGAAGGGGCT GATTGAGAAG TCAGCAGGGG ATGTGGACGC CCTGGCCTTT
    GACGGACAGA CCTACGTCGA GTACCTCAAT GCCGTGACTG AGAGTGAGAA GGCCCTGCAG
    AGCAACCACT TTGAGCTGAG CCTGCGCACG GAAGCCACTC AGGGGCTGGT GCTGTGGAGC
    GGCAAGGCCA CGGAGCGGGC CGACTATGTC GCACTGGCCA TCGTGGATGG GCGCCTGCAG
    CTGACCTATG ACCTGGGCTC CCAGCCCGTC GTGCTGCGCT CCACCGTGCC GGTCAACACC
    AACCGCTGGC TACGAGTCAG GGCACATAGG GAACAGAGGG AAGGTTCCCT TCAGGTGGGC
    AATGAGGCTC CCGTGACTGG CTCTTCCCCG CTGGGTGCCA CACAGCTAGA CACAGACGGA
    ACTCTATGGC TGGGTGGCCT GGAGAAGCTG CCCATAGGCC AGGCCCTGCC CAAGGCCTAT
    GGCACGGGCT TTGTGGGCTG CCTGCGGGAT GTGGTGGTGG GGCAGCGCCC ACTGCACCTG
    CTGGAGGATG CTGTCACCAA GCCAGAGCTC CGACCCTGTG CCGCCCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005620420.1
    CDS132..6302
    Translation

    Target ORF information:

    RefSeq Version XM_005620363.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris agrin (AGRN), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005620363.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
    GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG
    GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC
    CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC
    ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA
    GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC
    CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG
    ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC
    TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC
    AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT
    CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC
    TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG
    CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC
    CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC
    CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG
    CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG
    GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC
    CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG
    TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT
    GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT
    GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG
    GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA
    TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT
    GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG
    GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG
    TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT
    GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC
    TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT
    GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC
    TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG
    GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG
    GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG
    GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT
    GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG
    CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG
    CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC
    ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT
    GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT
    GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC
    TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG
    CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT
    GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT
    GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGACT CTGTTCTCCT TGCAGAGACT
    GTTGCTCCTG GGGCCCGCCC AACATCTGTA CCCAGGGCCA CCTCAGGGCT CAGCACGAGC
    AAGGCGCTGC CACCCGCCCC CAGCACCCTC CCCCTGGCTC CCAGCAGCAC CCCTCATAGC
    CGGCCCACGC CTCGACCCTC ACCAGGATCT CGGACCACTG CCAGCATTCC CAGGACTGTC
    ACGAGGCCTG TGCTGACCAT GCCCCCCACA GCCCCCGCCA CGGCAGCCAA CCTCGCCACA
    TCTGCTTTTG GCGAGTCTGG CAGTGCTGAC GGGAGCGGCG ATGAGGGGCT GAGTGGTGAC
    CTGGAGGCCA GTGGGACTGG CTCAGGGGGA CTCGAGCCCC CTGAAGGTGG CAGTGCTGTG
    ACCCCTGAGC CACCCATGGA GAGGGCTTCT TGCTACAACT CGCCTTTGGG CTGCTGCTCG
    GATGGCAAGA CGCCCTCGCT GGACGCAGAG GGTTCCAACT GTCCTGCCAC CAAGGCGTTC
    CAGGGTGTGT TGGAGCTTGA GGGCGTGGAG GGGCAAGAGC TGTTCTACAC GCCGGAGATG
    GCTGACCCCA AGTCAGAGCT TTTTGGGGAG ACTGCTAGGA GCATCGAGAG CACCCTGGAT
    GACCTCTTCC GGAACTCAGA GGTTAAGAAG GACTTCCGAA GCGTCCGGCT GAGGGACTTG
    GGGCCTGGCA GCTCAGTCCG AGCCATTGTG GATGTGCACT TTGACCCTGC CACAACCTTT
    AGGGCGCCTG ATGTGGGCCA AGCCCTGCTC CGGCAGATAC AGGCATCCCG GCGCCGGTCC
    CTGGGCGTAC GGCGGCCACT GCAGGAGCAC GTGCGCTTTA TGGACTTTGA CTGGTTTCCT
    GCGTTCTTCA CGGGAGCCAC CACAGGAGCA ACACCCGCCG CAGCCACGGC CAGAGCCACC
    ACGGTGGCCC ACCTGCCACC TGCTGGGACC CAGTGGGCCC TCTACCCCAG TCACACAAGC
    CGGCCTGTGG GCAGGACCAC AGCTCCACCC ACCACACGCC AGCCTCCCAC CACTGCTCCC
    CGTCGCGTGC CCGGACGCCG GCCCTTGCCC CCAGGCACCC AGCAGCCCCC GAAGCCCTGC
    GACTCCCAGC CCTGCCTCCA TGGGGGAACC TGCCAGGACC AGGACTCGGG TGGAGGCTTC
    ACCTGCAGCT GCCCAGTGGG CAGGAGGGGT GCCGTCTGTG AGAAGGCCCT GTACCTCTCG
    GTGCCAGCTT TCAATGGCCG CTCCTTCCTG GCCTTCCCCA CCCTCCGTGC CTACCACACG
    CTGCGCCTAG CACTTGAGTT CCGGGCACTA GAGCCACAGG GCCTGCTGCT CTATAATGGC
    AATGCCCAGG GCAAGGACTT CCTGGCATTG ACGCTGCTGG GGGGCCGTGT GCAGCTCAGG
    TTTGACACAG GTTCCGGGCC TGCAGTACTG ACCAGCTCAG TGCCTATAGA GCCTGGCCGC
    TGGCATCGCT TGGAGCTGTC TCGGCACTGG CGCCGGGGAA CACTTTCAGT GGATGGTGAG
    CCCCTGGTGC TGGGCCACAG CCCCAGCGGT ACAGATGGTC TCAACCTGGA CACAGACCTT
    TTTGTGGGCG GCATCCCTGA GGATCAGGCT TCTGTGGTAC TCGAAAGGAC GTCCATCAGC
    GTTGGCCTCA AGGGCTGCAT CCGCCTGCTG GATGTCAATA ATCAGCGTTT AGAGCTCAGC
    AGCTGGCCAG GGGCTGCAGC CCGAAGCTCC GGGGTGGGCG AGTGTGGAGA CCACCCCTGC
    CTGCCCAACC CCTGCCTCGA CGGGGCTCCG TGCCAGGCCC TGGAGGCTGG CATGTTCCAC
    TGTCAGTGCC CACCTGGCCG CTTTGGTCCA ACCTGTAGTG GTGACAAGAA CCCCTGTGAG
    CCGAACCCCT GCCATGGGGC AGCCCCCTGC CGTGTGCTGC CTGATGGGGA GGCCAAGTGT
    GAATGCCCCC GAGGACGAGG GGGTGCCATC TGCCAGACAG TCTCGGAGCG GGATTCTTCC
    CAGCCCTTCC TGGCTGACTT CCATGGCTTC TCCTACCTGG AGCTGAAAGG GTTGCATACC
    TTTGAGCGGG ACCTGGGGGA GAAGCTGGCA CTGGAGGTGG TGTTCCTGGC CCGTGGGCCC
    AGTGGTTTGC TCCTCTACAA TGGGCAGAAA ACAGACGGCA AGGGGGACTT TGTGTCACTG
    GCGTTGCATG ACCGCTTCTT GGAATTCCGC TATGACCTGG GCAAGGGAGC AGCGGTCATC
    AGGAGCAAGG AGCCGGTGGC CCTGGACACC TGGACCAGGG TCTCACTAGA GCGCAATGGC
    CGCAAGGGTG CCATGCGTGT CAGTGATGGG CCTCGCGTGC TGGGAGAGTC CCCGAAATCC
    CGCAAGGTGC CACACACTGT CCTCAACCTG AAGGAGCCAC TCTATGTTGG AGGGGCCCCT
    GACTTCAGCA AACTGGCCCG GGCAGCTGCG GTATTCTCTG GCTTCGATGG CGCCATCCAA
    CTGGTCTCCT TGAAAGGCCA CCAGCTGCTG TCCCAGGAGC ACGTGGTGCG GTCAGTGGAT
    GTCTCATCTT TTGCCCACCA CCCTTGTACT CAGGCCTCGG GCCACCCATG CCTCAATGGG
    GCCTCCTGCC TCCCTCGAGA GGCCTCCTAT GAGTGCCTGT GCCCCGGGGG CTTCTCAGGG
    CTTCACTGTG AGAAGGGGCT GATTGAGAAG TCAGCAGGGG ATGTGGACGC CCTGGCCTTT
    GACGGACAGA CCTACGTCGA GTACCTCAAT GCCGTGACTG AGAGTGAGAA GGCCCTGCAG
    AGCAACCACT TTGAGCTGAG CCTGCGCACG GAAGCCACTC AGGGGCTGGT GCTGTGGAGC
    GGCAAGGCCA CGGAGCGGGC CGACTATGTC GCACTGGCCA TCGTGGATGG GCGCCTGCAG
    CTGACCTATG ACCTGGGCTC CCAGCCCGTC GTGCTGCGCT CCACCGTGCC GGTCAACACC
    AACCGCTGGC TACGAGTCAG GGCACATAGG GAACAGAGGG AAGGTTCCCT TCAGGTGGGC
    AATGAGGCTC CCGTGACTGG CTCTTCCCCG CTGGGTGCCA CACAGCTAGA CACAGACGGA
    ACTCTATGGC TGGGTGGCCT GGAGAAGCTG CCCATAGGCC AGGCCCTGCC CAAGGCCTAT
    GGCACGGGCT TTGTGGGCTG CCTGCGGGAT GTGGTGGTGG GGCAGCGCCC ACTGCACCTG
    CTGGAGGATG CTGTCACCAA GCCAGAGCTC CGACCCTGTG CCGCCCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06698
    Clone ID Related Accession (Same CDS sequence) XM_005620364.1 , XM_005620364.2
    Accession Version XM_005620364.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6159bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product agrin isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726058.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
    GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG
    GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC
    CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC
    ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA
    GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC
    CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG
    ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC
    TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC
    AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT
    CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC
    TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG
    CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC
    CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC
    CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG
    CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG
    GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC
    CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG
    TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT
    GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT
    GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG
    GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA
    TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT
    GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG
    GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG
    TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT
    GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC
    TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT
    GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC
    TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG
    GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG
    GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG
    GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT
    GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG
    CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG
    CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC
    ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT
    GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT
    GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC
    TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG
    CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT
    GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT
    GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGACT CTGTTCTCCT TGCAGAGACT
    GTTGCTCCTG GGGCCCGCCC AACATCTGTA CCCAGGGCCA CCTCAGGGCT CAGCACGAGC
    AAGGCGCTGC CACCCGCCCC CAGCACCCTC CCCCTGGCTC CCAGCAGCAC CCCTCATAGC
    CGGCCCACGC CTCGACCCTC ACCAGGATCT CGGACCACTG CCAGCATTCC CAGGACTGTC
    ACGAGGCCTG TGCTGACCAT GCCCCCCACA GCCCCCGCCA CGGCAGCCAA CCTCGCCACA
    TCTGCTTTTG GCGAGTCTGG CAGTGCTGAC GGGAGCGGCG ATGAGGGGCT GAGTGGTGAC
    CTGGAGGCCA GTGGGACTGG CTCAGGGGGA CTCGAGCCCC CTGAAGGTGG CAGTGCTGTG
    ACCCCTGAGC CACCCATGGA GAGGGCTTCT TGCTACAACT CGCCTTTGGG CTGCTGCTCG
    GATGGCAAGA CGCCCTCGCT GGACGCAGAG GGTTCCAACT GTCCTGCCAC CAAGGCGTTC
    CAGGGTGTGT TGGAGCTTGA GGGCGTGGAG GGGCAAGAGC TGTTCTACAC GCCGGAGATG
    GCTGACCCCA AGTCAGAGCT TTTTGGGGAG ACTGCTAGGA GCATCGAGAG CACCCTGGAT
    GACCTCTTCC GGAACTCAGA GGTTAAGAAG GACTTCCGAA GCGTCCGGCT GAGGGACTTG
    GGGCCTGGCA GCTCAGTCCG AGCCATTGTG GATGTGCACT TTGACCCTGC CACAACCTTT
    AGGGCGCCTG ATGTGGGCCA AGCCCTGCTC CGGCAGATAC AGGCATCCCG GCGCCGGTCC
    CTGGGCGTAC GGCGGCCACT GCAGGAGCAC GTGCGCTTTA TGGACTTTGA CTGGTTTCCT
    GCGTTCTTCA CGGGAGCCAC CACAGGAGCA ACACCCGCCG CAGCCACGGC CAGAGCCACC
    ACGGTGGCCC ACCTGCCACC TGCTGGGACC CAGTGGGCCC TCTACCCCAG TCACACAAGC
    CGGCCTGTGG GCAGGACCAC AGCTCCACCC ACCACACGCC AGCCTCCCAC CACTGCTCCC
    CGTCGCGTGC CCGGACGCCG GCCCTTGCCC CCAGGCACCC AGCAGCCCCC GAAGCCCTGC
    GACTCCCAGC CCTGCCTCCA TGGGGGAACC TGCCAGGACC AGGACTCGGG TGGAGGCTTC
    ACCTGCAGCT GCCCAGTGGG CAGGAGGGGT GCCGTCTGTG AGAAGGCCCT GTACCTCTCG
    GTGCCAGCTT TCAATGGCCG CTCCTTCCTG GCCTTCCCCA CCCTCCGTGC CTACCACACG
    CTGCGCCTAG CACTTGAGTT CCGGGCACTA GAGCCACAGG GCCTGCTGCT CTATAATGGC
    AATGCCCAGG GCAAGGACTT CCTGGCATTG ACGCTGCTGG GGGGCCGTGT GCAGCTCAGG
    TTTGACACAG GTTCCGGGCC TGCAGTACTG ACCAGCTCAG TGCCTATAGA GCCTGGCCGC
    TGGCATCGCT TGGAGCTGTC TCGGCACTGG CGCCGGGGAA CACTTTCAGT GGATGGTGAG
    CCCCTGGTGC TGGGCCACAG CCCCAGCGGT ACAGATGGTC TCAACCTGGA CACAGACCTT
    TTTGTGGGCG GCATCCCTGA GGATCAGGCT TCTGTGGTAC TCGAAAGGAC GTCCATCAGC
    GTTGGCCTCA AGGGCTGCAT CCGCCTGCTG GATGTCAATA ATCAGCGTTT AGAGCTCAGC
    AGCTGGCCAG GGGCTGCAGC CCGAAGCTCC GGGGTGGGCG AGTGTGGAGA CCACCCCTGC
    CTGCCCAACC CCTGCCTCGA CGGGGCTCCG TGCCAGGCCC TGGAGGCTGG CATGTTCCAC
    TGTCAGTGCC CACCTGGCCG CTTTGGTCCA ACCTGTAGTG GTGACAAGAA CCCCTGTGAG
    CCGAACCCCT GCCATGGGGC AGCCCCCTGC CGTGTGCTGC CTGATGGGGA GGCCAAGTGT
    GAATGCCCCC GAGGACGAGG GGGTGCCATC TGCCAGACAG TCTCGGAGCG GGATTCTTCC
    CAGCCCTTCC TGGCTGACTT CCATGGCTTC TCCTACCTGG AGCTGAAAGG GTTGCATACC
    TTTGAGCGGG ACCTGGGGGA GAAGCTGGCA CTGGAGGTGG TGTTCCTGGC CCGTGGGCCC
    AGTGGTTTGC TCCTCTACAA TGGGCAGAAA ACAGACGGCA AGGGGGACTT TGTGTCACTG
    GCGTTGCATG ACCGCTTCTT GGAATTCCGC TATGACCTGG GCAAGGGAGC AGCGGTCATC
    AGGAGCAAGG AGCCGGTGGC CCTGGACACC TGGACCAGGG TCTCACTAGA GCGCAATGGC
    CGCAAGGGTG CCATGCGTGT CAGTGATGGG CCTCGCGTGC TGGGAGAGTC CCCGGTGCCA
    CACACTGTCC TCAACCTGAA GGAGCCACTC TATGTTGGAG GGGCCCCTGA CTTCAGCAAA
    CTGGCCCGGG CAGCTGCGGT ATTCTCTGGC TTCGATGGCG CCATCCAACT GGTCTCCTTG
    AAAGGCCACC AGCTGCTGTC CCAGGAGCAC GTGGTGCGGT CAGTGGATGT CTCATCTTTT
    GCCCACCACC CTTGTACTCA GGCCTCGGGC CACCCATGCC TCAATGGGGC CTCCTGCCTC
    CCTCGAGAGG CCTCCTATGA GTGCCTGTGC CCCGGGGGCT TCTCAGGGCT TCACTGTGAG
    AAGGGGCTGA TTGAGAAGTC AGCAGGGGAT GTGGACGCCC TGGCCTTTGA CGGACAGACC
    TACGTCGAGT ACCTCAATGC CGTGACTGAG AGTGAGAAGG CCCTGCAGAG CAACCACTTT
    GAGCTGAGCC TGCGCACGGA AGCCACTCAG GGGCTGGTGC TGTGGAGCGG CAAGGCCACG
    GAGCGGGCCG ACTATGTCGC ACTGGCCATC GTGGATGGGC GCCTGCAGCT GACCTATGAC
    CTGGGCTCCC AGCCCGTCGT GCTGCGCTCC ACCGTGCCGG TCAACACCAA CCGCTGGCTA
    CGAGTCAGGG CACATAGGGA ACAGAGGGAA GGTTCCCTTC AGGTGGGCAA TGAGGCTCCC
    GTGACTGGCT CTTCCCCGCT GGGTGCCACA CAGCTAGACA CAGACGGAAC TCTATGGCTG
    GGTGGCCTGG AGAAGCTGCC CATAGGCCAG GCCCTGCCCA AGGCCTATGG CACGGGCTTT
    GTGGGCTGCC TGCGGGATGT GGTGGTGGGG CAGCGCCCAC TGCACCTGCT GGAGGATGCT
    GTCACCAAGC CAGAGCTCCG ACCCTGTGCC GCCCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005620421.1
    CDS132..6290
    Translation

    Target ORF information:

    RefSeq Version XM_005620364.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris agrin (AGRN), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005620364.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
    GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG
    GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC
    CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC
    ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA
    GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC
    CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG
    ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC
    TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC
    AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT
    CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC
    TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG
    CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC
    CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC
    CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG
    CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG
    GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC
    CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG
    TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT
    GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT
    GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG
    GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA
    TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT
    GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG
    GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG
    TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT
    GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC
    TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT
    GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC
    TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG
    GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG
    GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG
    GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT
    GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG
    CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG
    CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC
    ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT
    GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT
    GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC
    TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG
    CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT
    GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT
    GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGACT CTGTTCTCCT TGCAGAGACT
    GTTGCTCCTG GGGCCCGCCC AACATCTGTA CCCAGGGCCA CCTCAGGGCT CAGCACGAGC
    AAGGCGCTGC CACCCGCCCC CAGCACCCTC CCCCTGGCTC CCAGCAGCAC CCCTCATAGC
    CGGCCCACGC CTCGACCCTC ACCAGGATCT CGGACCACTG CCAGCATTCC CAGGACTGTC
    ACGAGGCCTG TGCTGACCAT GCCCCCCACA GCCCCCGCCA CGGCAGCCAA CCTCGCCACA
    TCTGCTTTTG GCGAGTCTGG CAGTGCTGAC GGGAGCGGCG ATGAGGGGCT GAGTGGTGAC
    CTGGAGGCCA GTGGGACTGG CTCAGGGGGA CTCGAGCCCC CTGAAGGTGG CAGTGCTGTG
    ACCCCTGAGC CACCCATGGA GAGGGCTTCT TGCTACAACT CGCCTTTGGG CTGCTGCTCG
    GATGGCAAGA CGCCCTCGCT GGACGCAGAG GGTTCCAACT GTCCTGCCAC CAAGGCGTTC
    CAGGGTGTGT TGGAGCTTGA GGGCGTGGAG GGGCAAGAGC TGTTCTACAC GCCGGAGATG
    GCTGACCCCA AGTCAGAGCT TTTTGGGGAG ACTGCTAGGA GCATCGAGAG CACCCTGGAT
    GACCTCTTCC GGAACTCAGA GGTTAAGAAG GACTTCCGAA GCGTCCGGCT GAGGGACTTG
    GGGCCTGGCA GCTCAGTCCG AGCCATTGTG GATGTGCACT TTGACCCTGC CACAACCTTT
    AGGGCGCCTG ATGTGGGCCA AGCCCTGCTC CGGCAGATAC AGGCATCCCG GCGCCGGTCC
    CTGGGCGTAC GGCGGCCACT GCAGGAGCAC GTGCGCTTTA TGGACTTTGA CTGGTTTCCT
    GCGTTCTTCA CGGGAGCCAC CACAGGAGCA ACACCCGCCG CAGCCACGGC CAGAGCCACC
    ACGGTGGCCC ACCTGCCACC TGCTGGGACC CAGTGGGCCC TCTACCCCAG TCACACAAGC
    CGGCCTGTGG GCAGGACCAC AGCTCCACCC ACCACACGCC AGCCTCCCAC CACTGCTCCC
    CGTCGCGTGC CCGGACGCCG GCCCTTGCCC CCAGGCACCC AGCAGCCCCC GAAGCCCTGC
    GACTCCCAGC CCTGCCTCCA TGGGGGAACC TGCCAGGACC AGGACTCGGG TGGAGGCTTC
    ACCTGCAGCT GCCCAGTGGG CAGGAGGGGT GCCGTCTGTG AGAAGGCCCT GTACCTCTCG
    GTGCCAGCTT TCAATGGCCG CTCCTTCCTG GCCTTCCCCA CCCTCCGTGC CTACCACACG
    CTGCGCCTAG CACTTGAGTT CCGGGCACTA GAGCCACAGG GCCTGCTGCT CTATAATGGC
    AATGCCCAGG GCAAGGACTT CCTGGCATTG ACGCTGCTGG GGGGCCGTGT GCAGCTCAGG
    TTTGACACAG GTTCCGGGCC TGCAGTACTG ACCAGCTCAG TGCCTATAGA GCCTGGCCGC
    TGGCATCGCT TGGAGCTGTC TCGGCACTGG CGCCGGGGAA CACTTTCAGT GGATGGTGAG
    CCCCTGGTGC TGGGCCACAG CCCCAGCGGT ACAGATGGTC TCAACCTGGA CACAGACCTT
    TTTGTGGGCG GCATCCCTGA GGATCAGGCT TCTGTGGTAC TCGAAAGGAC GTCCATCAGC
    GTTGGCCTCA AGGGCTGCAT CCGCCTGCTG GATGTCAATA ATCAGCGTTT AGAGCTCAGC
    AGCTGGCCAG GGGCTGCAGC CCGAAGCTCC GGGGTGGGCG AGTGTGGAGA CCACCCCTGC
    CTGCCCAACC CCTGCCTCGA CGGGGCTCCG TGCCAGGCCC TGGAGGCTGG CATGTTCCAC
    TGTCAGTGCC CACCTGGCCG CTTTGGTCCA ACCTGTAGTG GTGACAAGAA CCCCTGTGAG
    CCGAACCCCT GCCATGGGGC AGCCCCCTGC CGTGTGCTGC CTGATGGGGA GGCCAAGTGT
    GAATGCCCCC GAGGACGAGG GGGTGCCATC TGCCAGACAG TCTCGGAGCG GGATTCTTCC
    CAGCCCTTCC TGGCTGACTT CCATGGCTTC TCCTACCTGG AGCTGAAAGG GTTGCATACC
    TTTGAGCGGG ACCTGGGGGA GAAGCTGGCA CTGGAGGTGG TGTTCCTGGC CCGTGGGCCC
    AGTGGTTTGC TCCTCTACAA TGGGCAGAAA ACAGACGGCA AGGGGGACTT TGTGTCACTG
    GCGTTGCATG ACCGCTTCTT GGAATTCCGC TATGACCTGG GCAAGGGAGC AGCGGTCATC
    AGGAGCAAGG AGCCGGTGGC CCTGGACACC TGGACCAGGG TCTCACTAGA GCGCAATGGC
    CGCAAGGGTG CCATGCGTGT CAGTGATGGG CCTCGCGTGC TGGGAGAGTC CCCGGTGCCA
    CACACTGTCC TCAACCTGAA GGAGCCACTC TATGTTGGAG GGGCCCCTGA CTTCAGCAAA
    CTGGCCCGGG CAGCTGCGGT ATTCTCTGGC TTCGATGGCG CCATCCAACT GGTCTCCTTG
    AAAGGCCACC AGCTGCTGTC CCAGGAGCAC GTGGTGCGGT CAGTGGATGT CTCATCTTTT
    GCCCACCACC CTTGTACTCA GGCCTCGGGC CACCCATGCC TCAATGGGGC CTCCTGCCTC
    CCTCGAGAGG CCTCCTATGA GTGCCTGTGC CCCGGGGGCT TCTCAGGGCT TCACTGTGAG
    AAGGGGCTGA TTGAGAAGTC AGCAGGGGAT GTGGACGCCC TGGCCTTTGA CGGACAGACC
    TACGTCGAGT ACCTCAATGC CGTGACTGAG AGTGAGAAGG CCCTGCAGAG CAACCACTTT
    GAGCTGAGCC TGCGCACGGA AGCCACTCAG GGGCTGGTGC TGTGGAGCGG CAAGGCCACG
    GAGCGGGCCG ACTATGTCGC ACTGGCCATC GTGGATGGGC GCCTGCAGCT GACCTATGAC
    CTGGGCTCCC AGCCCGTCGT GCTGCGCTCC ACCGTGCCGG TCAACACCAA CCGCTGGCTA
    CGAGTCAGGG CACATAGGGA ACAGAGGGAA GGTTCCCTTC AGGTGGGCAA TGAGGCTCCC
    GTGACTGGCT CTTCCCCGCT GGGTGCCACA CAGCTAGACA CAGACGGAAC TCTATGGCTG
    GGTGGCCTGG AGAAGCTGCC CATAGGCCAG GCCCTGCCCA AGGCCTATGG CACGGGCTTT
    GTGGGCTGCC TGCGGGATGT GGTGGTGGGG CAGCGCCCAC TGCACCTGCT GGAGGATGCT
    GTCACCAAGC CAGAGCTCCG ACCCTGTGCC GCCCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06698
    Clone ID Related Accession (Same CDS sequence) XM_005620364.1 , XM_005620364.2
    Accession Version XM_005620364.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6159bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product agrin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005620364.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
    GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG
    GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC
    CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC
    ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA
    GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC
    CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG
    ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC
    TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC
    AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT
    CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC
    TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG
    CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC
    CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC
    CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG
    CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG
    GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC
    CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG
    TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT
    GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT
    GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG
    GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA
    TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT
    GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG
    GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG
    TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT
    GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC
    TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT
    GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC
    TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG
    GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG
    GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG
    GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT
    GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG
    CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG
    CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC
    ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT
    GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT
    GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC
    TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG
    CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT
    GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT
    GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGACT CTGTTCTCCT TGCAGAGACT
    GTTGCTCCTG GGGCCCGCCC AACATCTGTA CCCAGGGCCA CCTCAGGGCT CAGCACGAGC
    AAGGCGCTGC CACCCGCCCC CAGCACCCTC CCCCTGGCTC CCAGCAGCAC CCCTCATAGC
    CGGCCCACGC CTCGACCCTC ACCAGGATCT CGGACCACTG CCAGCATTCC CAGGACTGTC
    ACGAGGCCTG TGCTGACCAT GCCCCCCACA GCCCCCGCCA CGGCAGCCAA CCTCGCCACA
    TCTGCTTTTG GCGAGTCTGG CAGTGCTGAC GGGAGCGGCG ATGAGGGGCT GAGTGGTGAC
    CTGGAGGCCA GTGGGACTGG CTCAGGGGGA CTCGAGCCCC CTGAAGGTGG CAGTGCTGTG
    ACCCCTGAGC CACCCATGGA GAGGGCTTCT TGCTACAACT CGCCTTTGGG CTGCTGCTCG
    GATGGCAAGA CGCCCTCGCT GGACGCAGAG GGTTCCAACT GTCCTGCCAC CAAGGCGTTC
    CAGGGTGTGT TGGAGCTTGA GGGCGTGGAG GGGCAAGAGC TGTTCTACAC GCCGGAGATG
    GCTGACCCCA AGTCAGAGCT TTTTGGGGAG ACTGCTAGGA GCATCGAGAG CACCCTGGAT
    GACCTCTTCC GGAACTCAGA GGTTAAGAAG GACTTCCGAA GCGTCCGGCT GAGGGACTTG
    GGGCCTGGCA GCTCAGTCCG AGCCATTGTG GATGTGCACT TTGACCCTGC CACAACCTTT
    AGGGCGCCTG ATGTGGGCCA AGCCCTGCTC CGGCAGATAC AGGCATCCCG GCGCCGGTCC
    CTGGGCGTAC GGCGGCCACT GCAGGAGCAC GTGCGCTTTA TGGACTTTGA CTGGTTTCCT
    GCGTTCTTCA CGGGAGCCAC CACAGGAGCA ACACCCGCCG CAGCCACGGC CAGAGCCACC
    ACGGTGGCCC ACCTGCCACC TGCTGGGACC CAGTGGGCCC TCTACCCCAG TCACACAAGC
    CGGCCTGTGG GCAGGACCAC AGCTCCACCC ACCACACGCC AGCCTCCCAC CACTGCTCCC
    CGTCGCGTGC CCGGACGCCG GCCCTTGCCC CCAGGCACCC AGCAGCCCCC GAAGCCCTGC
    GACTCCCAGC CCTGCCTCCA TGGGGGAACC TGCCAGGACC AGGACTCGGG TGGAGGCTTC
    ACCTGCAGCT GCCCAGTGGG CAGGAGGGGT GCCGTCTGTG AGAAGGCCCT GTACCTCTCG
    GTGCCAGCTT TCAATGGCCG CTCCTTCCTG GCCTTCCCCA CCCTCCGTGC CTACCACACG
    CTGCGCCTAG CACTTGAGTT CCGGGCACTA GAGCCACAGG GCCTGCTGCT CTATAATGGC
    AATGCCCAGG GCAAGGACTT CCTGGCATTG ACGCTGCTGG GGGGCCGTGT GCAGCTCAGG
    TTTGACACAG GTTCCGGGCC TGCAGTACTG ACCAGCTCAG TGCCTATAGA GCCTGGCCGC
    TGGCATCGCT TGGAGCTGTC TCGGCACTGG CGCCGGGGAA CACTTTCAGT GGATGGTGAG
    CCCCTGGTGC TGGGCCACAG CCCCAGCGGT ACAGATGGTC TCAACCTGGA CACAGACCTT
    TTTGTGGGCG GCATCCCTGA GGATCAGGCT TCTGTGGTAC TCGAAAGGAC GTCCATCAGC
    GTTGGCCTCA AGGGCTGCAT CCGCCTGCTG GATGTCAATA ATCAGCGTTT AGAGCTCAGC
    AGCTGGCCAG GGGCTGCAGC CCGAAGCTCC GGGGTGGGCG AGTGTGGAGA CCACCCCTGC
    CTGCCCAACC CCTGCCTCGA CGGGGCTCCG TGCCAGGCCC TGGAGGCTGG CATGTTCCAC
    TGTCAGTGCC CACCTGGCCG CTTTGGTCCA ACCTGTAGTG GTGACAAGAA CCCCTGTGAG
    CCGAACCCCT GCCATGGGGC AGCCCCCTGC CGTGTGCTGC CTGATGGGGA GGCCAAGTGT
    GAATGCCCCC GAGGACGAGG GGGTGCCATC TGCCAGACAG TCTCGGAGCG GGATTCTTCC
    CAGCCCTTCC TGGCTGACTT CCATGGCTTC TCCTACCTGG AGCTGAAAGG GTTGCATACC
    TTTGAGCGGG ACCTGGGGGA GAAGCTGGCA CTGGAGGTGG TGTTCCTGGC CCGTGGGCCC
    AGTGGTTTGC TCCTCTACAA TGGGCAGAAA ACAGACGGCA AGGGGGACTT TGTGTCACTG
    GCGTTGCATG ACCGCTTCTT GGAATTCCGC TATGACCTGG GCAAGGGAGC AGCGGTCATC
    AGGAGCAAGG AGCCGGTGGC CCTGGACACC TGGACCAGGG TCTCACTAGA GCGCAATGGC
    CGCAAGGGTG CCATGCGTGT CAGTGATGGG CCTCGCGTGC TGGGAGAGTC CCCGGTGCCA
    CACACTGTCC TCAACCTGAA GGAGCCACTC TATGTTGGAG GGGCCCCTGA CTTCAGCAAA
    CTGGCCCGGG CAGCTGCGGT ATTCTCTGGC TTCGATGGCG CCATCCAACT GGTCTCCTTG
    AAAGGCCACC AGCTGCTGTC CCAGGAGCAC GTGGTGCGGT CAGTGGATGT CTCATCTTTT
    GCCCACCACC CTTGTACTCA GGCCTCGGGC CACCCATGCC TCAATGGGGC CTCCTGCCTC
    CCTCGAGAGG CCTCCTATGA GTGCCTGTGC CCCGGGGGCT TCTCAGGGCT TCACTGTGAG
    AAGGGGCTGA TTGAGAAGTC AGCAGGGGAT GTGGACGCCC TGGCCTTTGA CGGACAGACC
    TACGTCGAGT ACCTCAATGC CGTGACTGAG AGTGAGAAGG CCCTGCAGAG CAACCACTTT
    GAGCTGAGCC TGCGCACGGA AGCCACTCAG GGGCTGGTGC TGTGGAGCGG CAAGGCCACG
    GAGCGGGCCG ACTATGTCGC ACTGGCCATC GTGGATGGGC GCCTGCAGCT GACCTATGAC
    CTGGGCTCCC AGCCCGTCGT GCTGCGCTCC ACCGTGCCGG TCAACACCAA CCGCTGGCTA
    CGAGTCAGGG CACATAGGGA ACAGAGGGAA GGTTCCCTTC AGGTGGGCAA TGAGGCTCCC
    GTGACTGGCT CTTCCCCGCT GGGTGCCACA CAGCTAGACA CAGACGGAAC TCTATGGCTG
    GGTGGCCTGG AGAAGCTGCC CATAGGCCAG GCCCTGCCCA AGGCCTATGG CACGGGCTTT
    GTGGGCTGCC TGCGGGATGT GGTGGTGGGG CAGCGCCCAC TGCACCTGCT GGAGGATGCT
    GTCACCAAGC CAGAGCTCCG ACCCTGTGCC GCCCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005620421.1
    CDS80..6238
    Translation

    Target ORF information:

    RefSeq Version XM_005620364.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris agrin (AGRN), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005620364.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
    GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG
    GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC
    CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC
    ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA
    GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC
    CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG
    ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC
    TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC
    AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT
    CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC
    TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG
    CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC
    CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC
    CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG
    CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG
    GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC
    CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG
    TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT
    GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT
    GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG
    GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA
    TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT
    GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG
    GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG
    TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT
    GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC
    TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT
    GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC
    TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG
    GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG
    GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG
    GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT
    GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG
    CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG
    CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC
    ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT
    GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT
    GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC
    TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG
    CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT
    GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT
    GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGACT CTGTTCTCCT TGCAGAGACT
    GTTGCTCCTG GGGCCCGCCC AACATCTGTA CCCAGGGCCA CCTCAGGGCT CAGCACGAGC
    AAGGCGCTGC CACCCGCCCC CAGCACCCTC CCCCTGGCTC CCAGCAGCAC CCCTCATAGC
    CGGCCCACGC CTCGACCCTC ACCAGGATCT CGGACCACTG CCAGCATTCC CAGGACTGTC
    ACGAGGCCTG TGCTGACCAT GCCCCCCACA GCCCCCGCCA CGGCAGCCAA CCTCGCCACA
    TCTGCTTTTG GCGAGTCTGG CAGTGCTGAC GGGAGCGGCG ATGAGGGGCT GAGTGGTGAC
    CTGGAGGCCA GTGGGACTGG CTCAGGGGGA CTCGAGCCCC CTGAAGGTGG CAGTGCTGTG
    ACCCCTGAGC CACCCATGGA GAGGGCTTCT TGCTACAACT CGCCTTTGGG CTGCTGCTCG
    GATGGCAAGA CGCCCTCGCT GGACGCAGAG GGTTCCAACT GTCCTGCCAC CAAGGCGTTC
    CAGGGTGTGT TGGAGCTTGA GGGCGTGGAG GGGCAAGAGC TGTTCTACAC GCCGGAGATG
    GCTGACCCCA AGTCAGAGCT TTTTGGGGAG ACTGCTAGGA GCATCGAGAG CACCCTGGAT
    GACCTCTTCC GGAACTCAGA GGTTAAGAAG GACTTCCGAA GCGTCCGGCT GAGGGACTTG
    GGGCCTGGCA GCTCAGTCCG AGCCATTGTG GATGTGCACT TTGACCCTGC CACAACCTTT
    AGGGCGCCTG ATGTGGGCCA AGCCCTGCTC CGGCAGATAC AGGCATCCCG GCGCCGGTCC
    CTGGGCGTAC GGCGGCCACT GCAGGAGCAC GTGCGCTTTA TGGACTTTGA CTGGTTTCCT
    GCGTTCTTCA CGGGAGCCAC CACAGGAGCA ACACCCGCCG CAGCCACGGC CAGAGCCACC
    ACGGTGGCCC ACCTGCCACC TGCTGGGACC CAGTGGGCCC TCTACCCCAG TCACACAAGC
    CGGCCTGTGG GCAGGACCAC AGCTCCACCC ACCACACGCC AGCCTCCCAC CACTGCTCCC
    CGTCGCGTGC CCGGACGCCG GCCCTTGCCC CCAGGCACCC AGCAGCCCCC GAAGCCCTGC
    GACTCCCAGC CCTGCCTCCA TGGGGGAACC TGCCAGGACC AGGACTCGGG TGGAGGCTTC
    ACCTGCAGCT GCCCAGTGGG CAGGAGGGGT GCCGTCTGTG AGAAGGCCCT GTACCTCTCG
    GTGCCAGCTT TCAATGGCCG CTCCTTCCTG GCCTTCCCCA CCCTCCGTGC CTACCACACG
    CTGCGCCTAG CACTTGAGTT CCGGGCACTA GAGCCACAGG GCCTGCTGCT CTATAATGGC
    AATGCCCAGG GCAAGGACTT CCTGGCATTG ACGCTGCTGG GGGGCCGTGT GCAGCTCAGG
    TTTGACACAG GTTCCGGGCC TGCAGTACTG ACCAGCTCAG TGCCTATAGA GCCTGGCCGC
    TGGCATCGCT TGGAGCTGTC TCGGCACTGG CGCCGGGGAA CACTTTCAGT GGATGGTGAG
    CCCCTGGTGC TGGGCCACAG CCCCAGCGGT ACAGATGGTC TCAACCTGGA CACAGACCTT
    TTTGTGGGCG GCATCCCTGA GGATCAGGCT TCTGTGGTAC TCGAAAGGAC GTCCATCAGC
    GTTGGCCTCA AGGGCTGCAT CCGCCTGCTG GATGTCAATA ATCAGCGTTT AGAGCTCAGC
    AGCTGGCCAG GGGCTGCAGC CCGAAGCTCC GGGGTGGGCG AGTGTGGAGA CCACCCCTGC
    CTGCCCAACC CCTGCCTCGA CGGGGCTCCG TGCCAGGCCC TGGAGGCTGG CATGTTCCAC
    TGTCAGTGCC CACCTGGCCG CTTTGGTCCA ACCTGTAGTG GTGACAAGAA CCCCTGTGAG
    CCGAACCCCT GCCATGGGGC AGCCCCCTGC CGTGTGCTGC CTGATGGGGA GGCCAAGTGT
    GAATGCCCCC GAGGACGAGG GGGTGCCATC TGCCAGACAG TCTCGGAGCG GGATTCTTCC
    CAGCCCTTCC TGGCTGACTT CCATGGCTTC TCCTACCTGG AGCTGAAAGG GTTGCATACC
    TTTGAGCGGG ACCTGGGGGA GAAGCTGGCA CTGGAGGTGG TGTTCCTGGC CCGTGGGCCC
    AGTGGTTTGC TCCTCTACAA TGGGCAGAAA ACAGACGGCA AGGGGGACTT TGTGTCACTG
    GCGTTGCATG ACCGCTTCTT GGAATTCCGC TATGACCTGG GCAAGGGAGC AGCGGTCATC
    AGGAGCAAGG AGCCGGTGGC CCTGGACACC TGGACCAGGG TCTCACTAGA GCGCAATGGC
    CGCAAGGGTG CCATGCGTGT CAGTGATGGG CCTCGCGTGC TGGGAGAGTC CCCGGTGCCA
    CACACTGTCC TCAACCTGAA GGAGCCACTC TATGTTGGAG GGGCCCCTGA CTTCAGCAAA
    CTGGCCCGGG CAGCTGCGGT ATTCTCTGGC TTCGATGGCG CCATCCAACT GGTCTCCTTG
    AAAGGCCACC AGCTGCTGTC CCAGGAGCAC GTGGTGCGGT CAGTGGATGT CTCATCTTTT
    GCCCACCACC CTTGTACTCA GGCCTCGGGC CACCCATGCC TCAATGGGGC CTCCTGCCTC
    CCTCGAGAGG CCTCCTATGA GTGCCTGTGC CCCGGGGGCT TCTCAGGGCT TCACTGTGAG
    AAGGGGCTGA TTGAGAAGTC AGCAGGGGAT GTGGACGCCC TGGCCTTTGA CGGACAGACC
    TACGTCGAGT ACCTCAATGC CGTGACTGAG AGTGAGAAGG CCCTGCAGAG CAACCACTTT
    GAGCTGAGCC TGCGCACGGA AGCCACTCAG GGGCTGGTGC TGTGGAGCGG CAAGGCCACG
    GAGCGGGCCG ACTATGTCGC ACTGGCCATC GTGGATGGGC GCCTGCAGCT GACCTATGAC
    CTGGGCTCCC AGCCCGTCGT GCTGCGCTCC ACCGTGCCGG TCAACACCAA CCGCTGGCTA
    CGAGTCAGGG CACATAGGGA ACAGAGGGAA GGTTCCCTTC AGGTGGGCAA TGAGGCTCCC
    GTGACTGGCT CTTCCCCGCT GGGTGCCACA CAGCTAGACA CAGACGGAAC TCTATGGCTG
    GGTGGCCTGG AGAAGCTGCC CATAGGCCAG GCCCTGCCCA AGGCCTATGG CACGGGCTTT
    GTGGGCTGCC TGCGGGATGT GGTGGTGGGG CAGCGCCCAC TGCACCTGCT GGAGGATGCT
    GTCACCAAGC CAGAGCTCCG ACCCTGTGCC GCCCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06699
    Clone ID Related Accession (Same CDS sequence) XM_536713.6 , XM_536713.5
    Accession Version XM_536713.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6141bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product agrin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_536713.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
    GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG
    GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC
    CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC
    ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA
    GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC
    CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG
    ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC
    TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC
    AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT
    CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC
    TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG
    CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC
    CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC
    CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG
    CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG
    GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC
    CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG
    TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT
    GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT
    GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG
    GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA
    TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT
    GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG
    GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG
    TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT
    GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC
    TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT
    GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC
    TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG
    GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG
    GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG
    GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT
    GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG
    CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG
    CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC
    ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT
    GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT
    GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC
    TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG
    CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT
    GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT
    GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGAGA CTGTTGCTCC TGGGGCCCGC
    CCAACATCTG TACCCAGGGC CACCTCAGGG CTCAGCACGA GCAAGGCGCT GCCACCCGCC
    CCCAGCACCC TCCCCCTGGC TCCCAGCAGC ACCCCTCATA GCCGGCCCAC GCCTCGACCC
    TCACCAGGAT CTCGGACCAC TGCCAGCATT CCCAGGACTG TCACGAGGCC TGTGCTGACC
    ATGCCCCCCA CAGCCCCCGC CACGGCAGCC AACCTCGCCA CATCTGCTTT TGGCGAGTCT
    GGCAGTGCTG ACGGGAGCGG CGATGAGGGG CTGAGTGGTG ACCTGGAGGC CAGTGGGACT
    GGCTCAGGGG GACTCGAGCC CCCTGAAGGT GGCAGTGCTG TGACCCCTGA GCCACCCATG
    GAGAGGGCTT CTTGCTACAA CTCGCCTTTG GGCTGCTGCT CGGATGGCAA GACGCCCTCG
    CTGGACGCAG AGGGTTCCAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GTTGGAGCTT
    GAGGGCGTGG AGGGGCAAGA GCTGTTCTAC ACGCCGGAGA TGGCTGACCC CAAGTCAGAG
    CTTTTTGGGG AGACTGCTAG GAGCATCGAG AGCACCCTGG ATGACCTCTT CCGGAACTCA
    GAGGTTAAGA AGGACTTCCG AAGCGTCCGG CTGAGGGACT TGGGGCCTGG CAGCTCAGTC
    CGAGCCATTG TGGATGTGCA CTTTGACCCT GCCACAACCT TTAGGGCGCC TGATGTGGGC
    CAAGCCCTGC TCCGGCAGAT ACAGGCATCC CGGCGCCGGT CCCTGGGCGT ACGGCGGCCA
    CTGCAGGAGC ACGTGCGCTT TATGGACTTT GACTGGTTTC CTGCGTTCTT CACGGGAGCC
    ACCACAGGAG CAACACCCGC CGCAGCCACG GCCAGAGCCA CCACGGTGGC CCACCTGCCA
    CCTGCTGGGA CCCAGTGGGC CCTCTACCCC AGTCACACAA GCCGGCCTGT GGGCAGGACC
    ACAGCTCCAC CCACCACACG CCAGCCTCCC ACCACTGCTC CCCGTCGCGT GCCCGGACGC
    CGGCCCTTGC CCCCAGGCAC CCAGCAGCCC CCGAAGCCCT GCGACTCCCA GCCCTGCCTC
    CATGGGGGAA CCTGCCAGGA CCAGGACTCG GGTGGAGGCT TCACCTGCAG CTGCCCAGTG
    GGCAGGAGGG GTGCCGTCTG TGAGAAGGCC CTGTACCTCT CGGTGCCAGC TTTCAATGGC
    CGCTCCTTCC TGGCCTTCCC CACCCTCCGT GCCTACCACA CGCTGCGCCT AGCACTTGAG
    TTCCGGGCAC TAGAGCCACA GGGCCTGCTG CTCTATAATG GCAATGCCCA GGGCAAGGAC
    TTCCTGGCAT TGACGCTGCT GGGGGGCCGT GTGCAGCTCA GGTTTGACAC AGGTTCCGGG
    CCTGCAGTAC TGACCAGCTC AGTGCCTATA GAGCCTGGCC GCTGGCATCG CTTGGAGCTG
    TCTCGGCACT GGCGCCGGGG AACACTTTCA GTGGATGGTG AGCCCCTGGT GCTGGGCCAC
    AGCCCCAGCG GTACAGATGG TCTCAACCTG GACACAGACC TTTTTGTGGG CGGCATCCCT
    GAGGATCAGG CTTCTGTGGT ACTCGAAAGG ACGTCCATCA GCGTTGGCCT CAAGGGCTGC
    ATCCGCCTGC TGGATGTCAA TAATCAGCGT TTAGAGCTCA GCAGCTGGCC AGGGGCTGCA
    GCCCGAAGCT CCGGGGTGGG CGAGTGTGGA GACCACCCCT GCCTGCCCAA CCCCTGCCTC
    GACGGGGCTC CGTGCCAGGC CCTGGAGGCT GGCATGTTCC ACTGTCAGTG CCCACCTGGC
    CGCTTTGGTC CAACCTGTAG TGGTGACAAG AACCCCTGTG AGCCGAACCC CTGCCATGGG
    GCAGCCCCCT GCCGTGTGCT GCCTGATGGG GAGGCCAAGT GTGAATGCCC CCGAGGACGA
    GGGGGTGCCA TCTGCCAGAC AGTCTCGGAG CGGGATTCTT CCCAGCCCTT CCTGGCTGAC
    TTCCATGGCT TCTCCTACCT GGAGCTGAAA GGGTTGCATA CCTTTGAGCG GGACCTGGGG
    GAGAAGCTGG CACTGGAGGT GGTGTTCCTG GCCCGTGGGC CCAGTGGTTT GCTCCTCTAC
    AATGGGCAGA AAACAGACGG CAAGGGGGAC TTTGTGTCAC TGGCGTTGCA TGACCGCTTC
    TTGGAATTCC GCTATGACCT GGGCAAGGGA GCAGCGGTCA TCAGGAGCAA GGAGCCGGTG
    GCCCTGGACA CCTGGACCAG GGTCTCACTA GAGCGCAATG GCCGCAAGGG TGCCATGCGT
    GTCAGTGATG GGCCTCGCGT GCTGGGAGAG TCCCCGGTGC CACACACTGT CCTCAACCTG
    AAGGAGCCAC TCTATGTTGG AGGGGCCCCT GACTTCAGCA AACTGGCCCG GGCAGCTGCG
    GTATTCTCTG GCTTCGATGG CGCCATCCAA CTGGTCTCCT TGAAAGGCCA CCAGCTGCTG
    TCCCAGGAGC ACGTGGTGCG GTCAGTGGAT GTCTCATCTT TTGCCCACCA CCCTTGTACT
    CAGGCCTCGG GCCACCCATG CCTCAATGGG GCCTCCTGCC TCCCTCGAGA GGCCTCCTAT
    GAGTGCCTGT GCCCCGGGGG CTTCTCAGGG CTTCACTGTG AGAAGGGGCT GATTGAGAAG
    TCAGCAGGGG ATGTGGACGC CCTGGCCTTT GACGGACAGA CCTACGTCGA GTACCTCAAT
    GCCGTGACTG AGAGTGAGAA GGCCCTGCAG AGCAACCACT TTGAGCTGAG CCTGCGCACG
    GAAGCCACTC AGGGGCTGGT GCTGTGGAGC GGCAAGGCCA CGGAGCGGGC CGACTATGTC
    GCACTGGCCA TCGTGGATGG GCGCCTGCAG CTGACCTATG ACCTGGGCTC CCAGCCCGTC
    GTGCTGCGCT CCACCGTGCC GGTCAACACC AACCGCTGGC TACGAGTCAG GGCACATAGG
    GAACAGAGGG AAGGTTCCCT TCAGGTGGGC AATGAGGCTC CCGTGACTGG CTCTTCCCCG
    CTGGGTGCCA CACAGCTAGA CACAGACGGA ACTCTATGGC TGGGTGGCCT GGAGAAGCTG
    CCCATAGGCC AGGCCCTGCC CAAGGCCTAT GGCACGGGCT TTGTGGGCTG CCTGCGGGAT
    GTGGTGGTGG GGCAGCGCCC ACTGCACCTG CTGGAGGATG CTGTCACCAA GCCAGAGCTC
    CGACCCTGTG CCGCCCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_536713.3
    CDS1..6141
    Translation

    Target ORF information:

    RefSeq Version XM_536713.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris agrin (AGRN), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_536713.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
    GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG
    GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC
    CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC
    ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA
    GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC
    CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG
    ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC
    TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC
    AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT
    CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC
    TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG
    CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC
    CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC
    CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG
    CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG
    GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC
    CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG
    TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT
    GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT
    GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG
    GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA
    TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT
    GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG
    GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG
    TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT
    GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC
    TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT
    GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC
    TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG
    GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG
    GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG
    GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT
    GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG
    CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG
    CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC
    ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT
    GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT
    GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC
    TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG
    CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT
    GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT
    GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGAGA CTGTTGCTCC TGGGGCCCGC
    CCAACATCTG TACCCAGGGC CACCTCAGGG CTCAGCACGA GCAAGGCGCT GCCACCCGCC
    CCCAGCACCC TCCCCCTGGC TCCCAGCAGC ACCCCTCATA GCCGGCCCAC GCCTCGACCC
    TCACCAGGAT CTCGGACCAC TGCCAGCATT CCCAGGACTG TCACGAGGCC TGTGCTGACC
    ATGCCCCCCA CAGCCCCCGC CACGGCAGCC AACCTCGCCA CATCTGCTTT TGGCGAGTCT
    GGCAGTGCTG ACGGGAGCGG CGATGAGGGG CTGAGTGGTG ACCTGGAGGC CAGTGGGACT
    GGCTCAGGGG GACTCGAGCC CCCTGAAGGT GGCAGTGCTG TGACCCCTGA GCCACCCATG
    GAGAGGGCTT CTTGCTACAA CTCGCCTTTG GGCTGCTGCT CGGATGGCAA GACGCCCTCG
    CTGGACGCAG AGGGTTCCAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GTTGGAGCTT
    GAGGGCGTGG AGGGGCAAGA GCTGTTCTAC ACGCCGGAGA TGGCTGACCC CAAGTCAGAG
    CTTTTTGGGG AGACTGCTAG GAGCATCGAG AGCACCCTGG ATGACCTCTT CCGGAACTCA
    GAGGTTAAGA AGGACTTCCG AAGCGTCCGG CTGAGGGACT TGGGGCCTGG CAGCTCAGTC
    CGAGCCATTG TGGATGTGCA CTTTGACCCT GCCACAACCT TTAGGGCGCC TGATGTGGGC
    CAAGCCCTGC TCCGGCAGAT ACAGGCATCC CGGCGCCGGT CCCTGGGCGT ACGGCGGCCA
    CTGCAGGAGC ACGTGCGCTT TATGGACTTT GACTGGTTTC CTGCGTTCTT CACGGGAGCC
    ACCACAGGAG CAACACCCGC CGCAGCCACG GCCAGAGCCA CCACGGTGGC CCACCTGCCA
    CCTGCTGGGA CCCAGTGGGC CCTCTACCCC AGTCACACAA GCCGGCCTGT GGGCAGGACC
    ACAGCTCCAC CCACCACACG CCAGCCTCCC ACCACTGCTC CCCGTCGCGT GCCCGGACGC
    CGGCCCTTGC CCCCAGGCAC CCAGCAGCCC CCGAAGCCCT GCGACTCCCA GCCCTGCCTC
    CATGGGGGAA CCTGCCAGGA CCAGGACTCG GGTGGAGGCT TCACCTGCAG CTGCCCAGTG
    GGCAGGAGGG GTGCCGTCTG TGAGAAGGCC CTGTACCTCT CGGTGCCAGC TTTCAATGGC
    CGCTCCTTCC TGGCCTTCCC CACCCTCCGT GCCTACCACA CGCTGCGCCT AGCACTTGAG
    TTCCGGGCAC TAGAGCCACA GGGCCTGCTG CTCTATAATG GCAATGCCCA GGGCAAGGAC
    TTCCTGGCAT TGACGCTGCT GGGGGGCCGT GTGCAGCTCA GGTTTGACAC AGGTTCCGGG
    CCTGCAGTAC TGACCAGCTC AGTGCCTATA GAGCCTGGCC GCTGGCATCG CTTGGAGCTG
    TCTCGGCACT GGCGCCGGGG AACACTTTCA GTGGATGGTG AGCCCCTGGT GCTGGGCCAC
    AGCCCCAGCG GTACAGATGG TCTCAACCTG GACACAGACC TTTTTGTGGG CGGCATCCCT
    GAGGATCAGG CTTCTGTGGT ACTCGAAAGG ACGTCCATCA GCGTTGGCCT CAAGGGCTGC
    ATCCGCCTGC TGGATGTCAA TAATCAGCGT TTAGAGCTCA GCAGCTGGCC AGGGGCTGCA
    GCCCGAAGCT CCGGGGTGGG CGAGTGTGGA GACCACCCCT GCCTGCCCAA CCCCTGCCTC
    GACGGGGCTC CGTGCCAGGC CCTGGAGGCT GGCATGTTCC ACTGTCAGTG CCCACCTGGC
    CGCTTTGGTC CAACCTGTAG TGGTGACAAG AACCCCTGTG AGCCGAACCC CTGCCATGGG
    GCAGCCCCCT GCCGTGTGCT GCCTGATGGG GAGGCCAAGT GTGAATGCCC CCGAGGACGA
    GGGGGTGCCA TCTGCCAGAC AGTCTCGGAG CGGGATTCTT CCCAGCCCTT CCTGGCTGAC
    TTCCATGGCT TCTCCTACCT GGAGCTGAAA GGGTTGCATA CCTTTGAGCG GGACCTGGGG
    GAGAAGCTGG CACTGGAGGT GGTGTTCCTG GCCCGTGGGC CCAGTGGTTT GCTCCTCTAC
    AATGGGCAGA AAACAGACGG CAAGGGGGAC TTTGTGTCAC TGGCGTTGCA TGACCGCTTC
    TTGGAATTCC GCTATGACCT GGGCAAGGGA GCAGCGGTCA TCAGGAGCAA GGAGCCGGTG
    GCCCTGGACA CCTGGACCAG GGTCTCACTA GAGCGCAATG GCCGCAAGGG TGCCATGCGT
    GTCAGTGATG GGCCTCGCGT GCTGGGAGAG TCCCCGGTGC CACACACTGT CCTCAACCTG
    AAGGAGCCAC TCTATGTTGG AGGGGCCCCT GACTTCAGCA AACTGGCCCG GGCAGCTGCG
    GTATTCTCTG GCTTCGATGG CGCCATCCAA CTGGTCTCCT TGAAAGGCCA CCAGCTGCTG
    TCCCAGGAGC ACGTGGTGCG GTCAGTGGAT GTCTCATCTT TTGCCCACCA CCCTTGTACT
    CAGGCCTCGG GCCACCCATG CCTCAATGGG GCCTCCTGCC TCCCTCGAGA GGCCTCCTAT
    GAGTGCCTGT GCCCCGGGGG CTTCTCAGGG CTTCACTGTG AGAAGGGGCT GATTGAGAAG
    TCAGCAGGGG ATGTGGACGC CCTGGCCTTT GACGGACAGA CCTACGTCGA GTACCTCAAT
    GCCGTGACTG AGAGTGAGAA GGCCCTGCAG AGCAACCACT TTGAGCTGAG CCTGCGCACG
    GAAGCCACTC AGGGGCTGGT GCTGTGGAGC GGCAAGGCCA CGGAGCGGGC CGACTATGTC
    GCACTGGCCA TCGTGGATGG GCGCCTGCAG CTGACCTATG ACCTGGGCTC CCAGCCCGTC
    GTGCTGCGCT CCACCGTGCC GGTCAACACC AACCGCTGGC TACGAGTCAG GGCACATAGG
    GAACAGAGGG AAGGTTCCCT TCAGGTGGGC AATGAGGCTC CCGTGACTGG CTCTTCCCCG
    CTGGGTGCCA CACAGCTAGA CACAGACGGA ACTCTATGGC TGGGTGGCCT GGAGAAGCTG
    CCCATAGGCC AGGCCCTGCC CAAGGCCTAT GGCACGGGCT TTGTGGGCTG CCTGCGGGAT
    GTGGTGGTGG GGCAGCGCCC ACTGCACCTG CTGGAGGATG CTGTCACCAA GCCAGAGCTC
    CGACCCTGTG CCGCCCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06699
    Clone ID Related Accession (Same CDS sequence) XM_536713.6 , XM_536713.5
    Accession Version XM_536713.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6141bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product agrin isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726058.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_536713.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
    GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG
    GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC
    CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC
    ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA
    GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC
    CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG
    ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC
    TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC
    AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT
    CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC
    TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG
    CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC
    CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC
    CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG
    CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG
    GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC
    CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG
    TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT
    GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT
    GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG
    GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA
    TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT
    GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG
    GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG
    TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT
    GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC
    TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT
    GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC
    TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG
    GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG
    GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG
    GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT
    GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG
    CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG
    CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC
    ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT
    GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT
    GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC
    TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG
    CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT
    GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT
    GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGAGA CTGTTGCTCC TGGGGCCCGC
    CCAACATCTG TACCCAGGGC CACCTCAGGG CTCAGCACGA GCAAGGCGCT GCCACCCGCC
    CCCAGCACCC TCCCCCTGGC TCCCAGCAGC ACCCCTCATA GCCGGCCCAC GCCTCGACCC
    TCACCAGGAT CTCGGACCAC TGCCAGCATT CCCAGGACTG TCACGAGGCC TGTGCTGACC
    ATGCCCCCCA CAGCCCCCGC CACGGCAGCC AACCTCGCCA CATCTGCTTT TGGCGAGTCT
    GGCAGTGCTG ACGGGAGCGG CGATGAGGGG CTGAGTGGTG ACCTGGAGGC CAGTGGGACT
    GGCTCAGGGG GACTCGAGCC CCCTGAAGGT GGCAGTGCTG TGACCCCTGA GCCACCCATG
    GAGAGGGCTT CTTGCTACAA CTCGCCTTTG GGCTGCTGCT CGGATGGCAA GACGCCCTCG
    CTGGACGCAG AGGGTTCCAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GTTGGAGCTT
    GAGGGCGTGG AGGGGCAAGA GCTGTTCTAC ACGCCGGAGA TGGCTGACCC CAAGTCAGAG
    CTTTTTGGGG AGACTGCTAG GAGCATCGAG AGCACCCTGG ATGACCTCTT CCGGAACTCA
    GAGGTTAAGA AGGACTTCCG AAGCGTCCGG CTGAGGGACT TGGGGCCTGG CAGCTCAGTC
    CGAGCCATTG TGGATGTGCA CTTTGACCCT GCCACAACCT TTAGGGCGCC TGATGTGGGC
    CAAGCCCTGC TCCGGCAGAT ACAGGCATCC CGGCGCCGGT CCCTGGGCGT ACGGCGGCCA
    CTGCAGGAGC ACGTGCGCTT TATGGACTTT GACTGGTTTC CTGCGTTCTT CACGGGAGCC
    ACCACAGGAG CAACACCCGC CGCAGCCACG GCCAGAGCCA CCACGGTGGC CCACCTGCCA
    CCTGCTGGGA CCCAGTGGGC CCTCTACCCC AGTCACACAA GCCGGCCTGT GGGCAGGACC
    ACAGCTCCAC CCACCACACG CCAGCCTCCC ACCACTGCTC CCCGTCGCGT GCCCGGACGC
    CGGCCCTTGC CCCCAGGCAC CCAGCAGCCC CCGAAGCCCT GCGACTCCCA GCCCTGCCTC
    CATGGGGGAA CCTGCCAGGA CCAGGACTCG GGTGGAGGCT TCACCTGCAG CTGCCCAGTG
    GGCAGGAGGG GTGCCGTCTG TGAGAAGGCC CTGTACCTCT CGGTGCCAGC TTTCAATGGC
    CGCTCCTTCC TGGCCTTCCC CACCCTCCGT GCCTACCACA CGCTGCGCCT AGCACTTGAG
    TTCCGGGCAC TAGAGCCACA GGGCCTGCTG CTCTATAATG GCAATGCCCA GGGCAAGGAC
    TTCCTGGCAT TGACGCTGCT GGGGGGCCGT GTGCAGCTCA GGTTTGACAC AGGTTCCGGG
    CCTGCAGTAC TGACCAGCTC AGTGCCTATA GAGCCTGGCC GCTGGCATCG CTTGGAGCTG
    TCTCGGCACT GGCGCCGGGG AACACTTTCA GTGGATGGTG AGCCCCTGGT GCTGGGCCAC
    AGCCCCAGCG GTACAGATGG TCTCAACCTG GACACAGACC TTTTTGTGGG CGGCATCCCT
    GAGGATCAGG CTTCTGTGGT ACTCGAAAGG ACGTCCATCA GCGTTGGCCT CAAGGGCTGC
    ATCCGCCTGC TGGATGTCAA TAATCAGCGT TTAGAGCTCA GCAGCTGGCC AGGGGCTGCA
    GCCCGAAGCT CCGGGGTGGG CGAGTGTGGA GACCACCCCT GCCTGCCCAA CCCCTGCCTC
    GACGGGGCTC CGTGCCAGGC CCTGGAGGCT GGCATGTTCC ACTGTCAGTG CCCACCTGGC
    CGCTTTGGTC CAACCTGTAG TGGTGACAAG AACCCCTGTG AGCCGAACCC CTGCCATGGG
    GCAGCCCCCT GCCGTGTGCT GCCTGATGGG GAGGCCAAGT GTGAATGCCC CCGAGGACGA
    GGGGGTGCCA TCTGCCAGAC AGTCTCGGAG CGGGATTCTT CCCAGCCCTT CCTGGCTGAC
    TTCCATGGCT TCTCCTACCT GGAGCTGAAA GGGTTGCATA CCTTTGAGCG GGACCTGGGG
    GAGAAGCTGG CACTGGAGGT GGTGTTCCTG GCCCGTGGGC CCAGTGGTTT GCTCCTCTAC
    AATGGGCAGA AAACAGACGG CAAGGGGGAC TTTGTGTCAC TGGCGTTGCA TGACCGCTTC
    TTGGAATTCC GCTATGACCT GGGCAAGGGA GCAGCGGTCA TCAGGAGCAA GGAGCCGGTG
    GCCCTGGACA CCTGGACCAG GGTCTCACTA GAGCGCAATG GCCGCAAGGG TGCCATGCGT
    GTCAGTGATG GGCCTCGCGT GCTGGGAGAG TCCCCGGTGC CACACACTGT CCTCAACCTG
    AAGGAGCCAC TCTATGTTGG AGGGGCCCCT GACTTCAGCA AACTGGCCCG GGCAGCTGCG
    GTATTCTCTG GCTTCGATGG CGCCATCCAA CTGGTCTCCT TGAAAGGCCA CCAGCTGCTG
    TCCCAGGAGC ACGTGGTGCG GTCAGTGGAT GTCTCATCTT TTGCCCACCA CCCTTGTACT
    CAGGCCTCGG GCCACCCATG CCTCAATGGG GCCTCCTGCC TCCCTCGAGA GGCCTCCTAT
    GAGTGCCTGT GCCCCGGGGG CTTCTCAGGG CTTCACTGTG AGAAGGGGCT GATTGAGAAG
    TCAGCAGGGG ATGTGGACGC CCTGGCCTTT GACGGACAGA CCTACGTCGA GTACCTCAAT
    GCCGTGACTG AGAGTGAGAA GGCCCTGCAG AGCAACCACT TTGAGCTGAG CCTGCGCACG
    GAAGCCACTC AGGGGCTGGT GCTGTGGAGC GGCAAGGCCA CGGAGCGGGC CGACTATGTC
    GCACTGGCCA TCGTGGATGG GCGCCTGCAG CTGACCTATG ACCTGGGCTC CCAGCCCGTC
    GTGCTGCGCT CCACCGTGCC GGTCAACACC AACCGCTGGC TACGAGTCAG GGCACATAGG
    GAACAGAGGG AAGGTTCCCT TCAGGTGGGC AATGAGGCTC CCGTGACTGG CTCTTCCCCG
    CTGGGTGCCA CACAGCTAGA CACAGACGGA ACTCTATGGC TGGGTGGCCT GGAGAAGCTG
    CCCATAGGCC AGGCCCTGCC CAAGGCCTAT GGCACGGGCT TTGTGGGCTG CCTGCGGGAT
    GTGGTGGTGG GGCAGCGCCC ACTGCACCTG CTGGAGGATG CTGTCACCAA GCCAGAGCTC
    CGACCCTGTG CCGCCCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_536713.3
    CDS132..6272
    Translation

    Target ORF information:

    RefSeq Version XM_536713.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris agrin (AGRN), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_536713.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
    GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG
    GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC
    CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC
    ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA
    GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC
    CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG
    ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC
    TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC
    AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT
    CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC
    TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG
    CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC
    CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC
    CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG
    CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG
    GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC
    CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG
    TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT
    GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT
    GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG
    GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA
    TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT
    GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG
    GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG
    TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT
    GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC
    TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT
    GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC
    TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG
    GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG
    GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG
    GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT
    GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG
    CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG
    CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC
    ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT
    GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT
    GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC
    TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG
    CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT
    GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT
    GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGAGA CTGTTGCTCC TGGGGCCCGC
    CCAACATCTG TACCCAGGGC CACCTCAGGG CTCAGCACGA GCAAGGCGCT GCCACCCGCC
    CCCAGCACCC TCCCCCTGGC TCCCAGCAGC ACCCCTCATA GCCGGCCCAC GCCTCGACCC
    TCACCAGGAT CTCGGACCAC TGCCAGCATT CCCAGGACTG TCACGAGGCC TGTGCTGACC
    ATGCCCCCCA CAGCCCCCGC CACGGCAGCC AACCTCGCCA CATCTGCTTT TGGCGAGTCT
    GGCAGTGCTG ACGGGAGCGG CGATGAGGGG CTGAGTGGTG ACCTGGAGGC CAGTGGGACT
    GGCTCAGGGG GACTCGAGCC CCCTGAAGGT GGCAGTGCTG TGACCCCTGA GCCACCCATG
    GAGAGGGCTT CTTGCTACAA CTCGCCTTTG GGCTGCTGCT CGGATGGCAA GACGCCCTCG
    CTGGACGCAG AGGGTTCCAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GTTGGAGCTT
    GAGGGCGTGG AGGGGCAAGA GCTGTTCTAC ACGCCGGAGA TGGCTGACCC CAAGTCAGAG
    CTTTTTGGGG AGACTGCTAG GAGCATCGAG AGCACCCTGG ATGACCTCTT CCGGAACTCA
    GAGGTTAAGA AGGACTTCCG AAGCGTCCGG CTGAGGGACT TGGGGCCTGG CAGCTCAGTC
    CGAGCCATTG TGGATGTGCA CTTTGACCCT GCCACAACCT TTAGGGCGCC TGATGTGGGC
    CAAGCCCTGC TCCGGCAGAT ACAGGCATCC CGGCGCCGGT CCCTGGGCGT ACGGCGGCCA
    CTGCAGGAGC ACGTGCGCTT TATGGACTTT GACTGGTTTC CTGCGTTCTT CACGGGAGCC
    ACCACAGGAG CAACACCCGC CGCAGCCACG GCCAGAGCCA CCACGGTGGC CCACCTGCCA
    CCTGCTGGGA CCCAGTGGGC CCTCTACCCC AGTCACACAA GCCGGCCTGT GGGCAGGACC
    ACAGCTCCAC CCACCACACG CCAGCCTCCC ACCACTGCTC CCCGTCGCGT GCCCGGACGC
    CGGCCCTTGC CCCCAGGCAC CCAGCAGCCC CCGAAGCCCT GCGACTCCCA GCCCTGCCTC
    CATGGGGGAA CCTGCCAGGA CCAGGACTCG GGTGGAGGCT TCACCTGCAG CTGCCCAGTG
    GGCAGGAGGG GTGCCGTCTG TGAGAAGGCC CTGTACCTCT CGGTGCCAGC TTTCAATGGC
    CGCTCCTTCC TGGCCTTCCC CACCCTCCGT GCCTACCACA CGCTGCGCCT AGCACTTGAG
    TTCCGGGCAC TAGAGCCACA GGGCCTGCTG CTCTATAATG GCAATGCCCA GGGCAAGGAC
    TTCCTGGCAT TGACGCTGCT GGGGGGCCGT GTGCAGCTCA GGTTTGACAC AGGTTCCGGG
    CCTGCAGTAC TGACCAGCTC AGTGCCTATA GAGCCTGGCC GCTGGCATCG CTTGGAGCTG
    TCTCGGCACT GGCGCCGGGG AACACTTTCA GTGGATGGTG AGCCCCTGGT GCTGGGCCAC
    AGCCCCAGCG GTACAGATGG TCTCAACCTG GACACAGACC TTTTTGTGGG CGGCATCCCT
    GAGGATCAGG CTTCTGTGGT ACTCGAAAGG ACGTCCATCA GCGTTGGCCT CAAGGGCTGC
    ATCCGCCTGC TGGATGTCAA TAATCAGCGT TTAGAGCTCA GCAGCTGGCC AGGGGCTGCA
    GCCCGAAGCT CCGGGGTGGG CGAGTGTGGA GACCACCCCT GCCTGCCCAA CCCCTGCCTC
    GACGGGGCTC CGTGCCAGGC CCTGGAGGCT GGCATGTTCC ACTGTCAGTG CCCACCTGGC
    CGCTTTGGTC CAACCTGTAG TGGTGACAAG AACCCCTGTG AGCCGAACCC CTGCCATGGG
    GCAGCCCCCT GCCGTGTGCT GCCTGATGGG GAGGCCAAGT GTGAATGCCC CCGAGGACGA
    GGGGGTGCCA TCTGCCAGAC AGTCTCGGAG CGGGATTCTT CCCAGCCCTT CCTGGCTGAC
    TTCCATGGCT TCTCCTACCT GGAGCTGAAA GGGTTGCATA CCTTTGAGCG GGACCTGGGG
    GAGAAGCTGG CACTGGAGGT GGTGTTCCTG GCCCGTGGGC CCAGTGGTTT GCTCCTCTAC
    AATGGGCAGA AAACAGACGG CAAGGGGGAC TTTGTGTCAC TGGCGTTGCA TGACCGCTTC
    TTGGAATTCC GCTATGACCT GGGCAAGGGA GCAGCGGTCA TCAGGAGCAA GGAGCCGGTG
    GCCCTGGACA CCTGGACCAG GGTCTCACTA GAGCGCAATG GCCGCAAGGG TGCCATGCGT
    GTCAGTGATG GGCCTCGCGT GCTGGGAGAG TCCCCGGTGC CACACACTGT CCTCAACCTG
    AAGGAGCCAC TCTATGTTGG AGGGGCCCCT GACTTCAGCA AACTGGCCCG GGCAGCTGCG
    GTATTCTCTG GCTTCGATGG CGCCATCCAA CTGGTCTCCT TGAAAGGCCA CCAGCTGCTG
    TCCCAGGAGC ACGTGGTGCG GTCAGTGGAT GTCTCATCTT TTGCCCACCA CCCTTGTACT
    CAGGCCTCGG GCCACCCATG CCTCAATGGG GCCTCCTGCC TCCCTCGAGA GGCCTCCTAT
    GAGTGCCTGT GCCCCGGGGG CTTCTCAGGG CTTCACTGTG AGAAGGGGCT GATTGAGAAG
    TCAGCAGGGG ATGTGGACGC CCTGGCCTTT GACGGACAGA CCTACGTCGA GTACCTCAAT
    GCCGTGACTG AGAGTGAGAA GGCCCTGCAG AGCAACCACT TTGAGCTGAG CCTGCGCACG
    GAAGCCACTC AGGGGCTGGT GCTGTGGAGC GGCAAGGCCA CGGAGCGGGC CGACTATGTC
    GCACTGGCCA TCGTGGATGG GCGCCTGCAG CTGACCTATG ACCTGGGCTC CCAGCCCGTC
    GTGCTGCGCT CCACCGTGCC GGTCAACACC AACCGCTGGC TACGAGTCAG GGCACATAGG
    GAACAGAGGG AAGGTTCCCT TCAGGTGGGC AATGAGGCTC CCGTGACTGG CTCTTCCCCG
    CTGGGTGCCA CACAGCTAGA CACAGACGGA ACTCTATGGC TGGGTGGCCT GGAGAAGCTG
    CCCATAGGCC AGGCCCTGCC CAAGGCCTAT GGCACGGGCT TTGTGGGCTG CCTGCGGGAT
    GTGGTGGTGG GGCAGCGCCC ACTGCACCTG CTGGAGGATG CTGTCACCAA GCCAGAGCTC
    CGACCCTGTG CCGCCCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.