MCF2L2 cDNA ORF clone, Canis lupus familiaris(dog)

The following MCF2L2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MCF2L2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe284493 XM_022414335.1
Latest version!
Canis lupus familiaris MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe284494 XM_022414336.1
Latest version!
Canis lupus familiaris MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe284495 XM_022414337.1
Latest version!
Canis lupus familiaris MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe284496 XM_022414340.1
Latest version!
Canis lupus familiaris MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe284496 XM_022414338.1
Latest version!
Canis lupus familiaris MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe284496 XM_022414339.1
Latest version!
Canis lupus familiaris MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe284497 XM_022414341.1
Latest version!
Canis lupus familiaris MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe30436 XM_005639762.2
Latest version!
Canis lupus familiaris MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe30436 XM_022414334.1
Latest version!
Canis lupus familiaris MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe284493
    Clone ID Related Accession (Same CDS sequence) XM_022414335.1
    Accession Version XM_022414335.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3393bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product probable guanine nucleotide exchange factor MCF2L2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTATTTT GGTTAAAGGA AGAGATGGCC CCCCAGGAGC TGAGCCGGCG ACTGGCCACA 
    GTGATCACTC ATGTCGATGA AATTATGCAG CAGGAAATCA GGCCCTCAGT GGCAGTGGAT
    ATAATTGAAC AACTTCACAG ACAGTTTGCC ATTCTCTCAG GAGGTCGAGG GAAAGATGGT
    GCACCCATCA TCACTTTCCC AGAGTTTGTG GGGTTCAAAC ACATCCCAGA GGAAGACTTT
    CTGAATGTCA TGACCTACCT GACTAGCATC CCCAGTGTGG AGGCTGCCAG CATTGGATTC
    ATCATTGTTA TTGACCGACG AAGGGACAAG TGGAGCTCCA TAAAAGCATC ATTGACACGA
    ATAGCTGTAG CATTTCCAGG AAACTTGCAG CTCATATTCA TCCTTCGTCC ATCTCGCTTT
    ATCCAGAGGA CATTCACTGA CATTGGCATT AAATATTATC GAGATGAATT TAAAATGAAA
    GTTCCGGCCA TTGAAAATTT TGCCATGACA TTGAAGACCA CTGCCCAGAT GCTTCAGATG
    TTTGGGGCCT GCCTAACCAC AACAGAGCTG CCCACAGGTG TGCTGTCCAC GGAAGACCTC
    CTCATGTCCC ACACAAGGCA GCAGGACAAG CTGCAGGATG AGCTGAAATT ACTTGGAAAG
    CAAGGGACCA CATTGCTGTC GTGTATTCAA GAACCAGCGA CCAAAAGTTC CACCAGCAAA
    CTCAATCCCA ATGAACTAGA GAATGTAGCT ACCATTGAAA GGTTATTACT TCAGTTGGAT
    GAGACAGAAA AAGCCTTTAA TCAGTTTTGG TCTGAGCATC ACTCAAAGCT TAACCAATGC
    CTACAACTAC AACACTTTGA GCACAATTTT TGTGAGGTTA AGCTTGCCCT GGACAATTTG
    TTGGCAGAGC AAGCGGAGTT TACAGACATT GGAGACAGTG TGATTCGTGT GGAGCAAATA
    CTTAAGGAAC ACAAAAAACT GGAAGAAAAA GGCCAGGAGC CCTTGGAAAA GGCCCAGCTG
    CTTGCAATAA TTGGGGACCA GCTCATCCAA AGCCACCACT ATGCAATGGA TTCCATCAGA
    CCGAGATGTG TAGAGCTCAG GCACCTCTGT GATGACTTCA TCAATGAAAA CAAGAAAAAA
    CATGACATTT TACAAAAGTC TTTGGAGCTG CATAGACAGC TGGACAAGGT CAGCCAGTGG
    TGTGAAACAG GAATCTACCT TTTGGCTTCC CAAGCTGTAG ACAAGTGCCA GTCACGAGAG
    GGGGTTGACG TTGCCTTGAA TGACATCGAG GCATTCCTGA GTACGGCGAA GGAGTACCAG
    TTGCCCAGCC ACATGGAATT TTACAGCCAG TTTGAGTTGA TACTCACCAT TGATGTAAAG
    GCCAAAGCCC AGGAAGTACT GCAGAAGCTG TGCGATGTAC AGGAAATATT TCACAAGAGG
    CAAATGAGTT TGATGAAACT GGCAGCCAGA CAGACTCGCC CAGTGCAACC TGTGGCCCCT
    CATCCTGAGT CCTCCCCAAA ATGGATGTCA CCAAAAACCA GCCAGCCCTT CACCTTGGCT
    CCTCTTCAGA GAACATCTGA GGAAACTTAT ACAGGGACAT TAGAAAAGGA AGATGATGAG
    ACTAAATTTG AAGTCAAGAA TGAAGAAATA CTGGAAAGCA GTCATGATGG GGAGAACCCT
    GGACAAGAGC AGCTTGAGAG TCCTGAAAAT CTTTCCCCTA CCAGGCGCAT TATACATGAC
    TTGCTTGAGA CGGAAGAGAT TTATATAAAA GAGATTAAGA GCATAATTGA TGGATATATC
    ACTCCAATGG ATTTTATTTG GCTGAAGCAT CTGATTCCAG ACGTTCTTCA GAATAACAAG
    GAGTTTCTCT TTGGGAATAT TAGAGAACTT TATGAATTTC ACAACAGGAC TTTTCTGAAA
    GAACTGGAAA AGTGCACTGA AAACCCCGAA ATGCTGGCAC GCTGCTTTCT CGAGAGAAAA
    GAAGATCTTC AAATATATTT TAAATACCAT AAGAATCTGC CCCGAGCTAG AGCAATCTGG
    CAAGAATGTC AAGACTGCAC CTTCTTTGGG GTTTGCCAGC GCCAACTGGA TCACAATCTC
    CCTCTTTTTA AGTATCTCAG AGGACCAAGC CAGAGACTTA TAAAATATCA GATGCTGCTG
    AAGGGTCTGC TGGATTTTGA GTCTCCTGAG GATTTGGGAA TAGACACAGG TGACCAGGAA
    GGAAGCTTGG CTAAGAATGG GCCAAAGAGA ACCAAGGATT CAGCTTTCTC AGCCGAACTA
    CAACAGGCTT TGGCCATGAT GGAAGATTTG ATCAAGTCCT GTGAATTGGC CGTGGACCTG
    GCAGCTGTCA CTGGATGTCC GGATGATATC AGGAAACTGG GAAAGCTACT GAAGCATGGC
    TCTTTCAACG TGTGGACAAT TCACAAGGAT CGTTATAAAA TGAAGGATTT TATTCGATTT
    AAGCCCAGAC AGAGGCAAAT CTATCTATTT GAAAGGGGAA TTGTATTCTG TAAGATACGA
    ATGGAGCCCA GTGACCAGGG CCTATCTCCT CATTACAGCT TTAAGAAATC TATGAAGTTG
    ATGACACTTT CAATTCGCCA GCTTGGAAGG GGGAGCAACC GGAAGTTTGA ACTTGCCAAC
    CGGAATGGAC TCGAGAAATA CATCCTGCAG GCAGCTTCGA AAGAAATCAG AGATTGTTGG
    TTTTCAGAAA TAAGTAAATT ACTGATGGAA CAACAAAACA ATATTAAAGA TATTTATGAA
    GATGAAGAAA ATGGCAAACA AATCTCCCCC AAATGCTGCT CCAGCAGCGA GTGTATCTCC
    ATGGAAACCT TTGAGGAGCG GAAAGGTGCA GAAGATATGG AAAAGGAGAG CAGTGCTCTG
    AGTCTATCCG GACTCTTCCA GTTGGACGAC AGTTACGAAA CGTGTTCCTC CAAACCTGTC
    CTGGCCACAG GAGAAAGCAT CCCGGGAAAA AGAGAAGGTG GAGACGAGGA CATGAAGCCT
    TCAGGCATCG CCACATTTCT CCTGGGGTGG GAGTGCGGAG ATCCGTTGAG AACCTCCTAT
    CGGAGCCTTG GTGGAAGCGG GTCGCTCTTC TCCCAGGATC CCCCCAAGAA TGTTGGCGTG
    GTGTTTGTTA CTTTGTTCCC GGATTCTATC TTTTCAAGTG GGAACTTCTT TCTTAAGCAC
    CCGAGGATTG TCTCCTGTGT GACTCAAGAA AATCACTTCA GAATTCAAGA AAACAAAAAT
    AAGCATCTCA CTTTATTCAT AAAGAAGCCC TTGACTTCGT GGCCAAAAAA AGGGCAGAGA
    CTAAGGGACA GACGATCCGG GACAGGCTCT TCAGCAAAAT TGGCCAACAG AGTGTTCTTT
    GAAATTTTTC AGAAAGTTCT GTTGTGGAGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022270043.1
    CDS436..3828
    Translation

    Target ORF information:

    RefSeq Version XM_022414335.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022414335.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTATTTT GGTTAAAGGA AGAGATGGCC CCCCAGGAGC TGAGCCGGCG ACTGGCCACA 
    GTGATCACTC ATGTCGATGA AATTATGCAG CAGGAAATCA GGCCCTCAGT GGCAGTGGAT
    ATAATTGAAC AACTTCACAG ACAGTTTGCC ATTCTCTCAG GAGGTCGAGG GAAAGATGGT
    GCACCCATCA TCACTTTCCC AGAGTTTGTG GGGTTCAAAC ACATCCCAGA GGAAGACTTT
    CTGAATGTCA TGACCTACCT GACTAGCATC CCCAGTGTGG AGGCTGCCAG CATTGGATTC
    ATCATTGTTA TTGACCGACG AAGGGACAAG TGGAGCTCCA TAAAAGCATC ATTGACACGA
    ATAGCTGTAG CATTTCCAGG AAACTTGCAG CTCATATTCA TCCTTCGTCC ATCTCGCTTT
    ATCCAGAGGA CATTCACTGA CATTGGCATT AAATATTATC GAGATGAATT TAAAATGAAA
    GTTCCGGCCA TTGAAAATTT TGCCATGACA TTGAAGACCA CTGCCCAGAT GCTTCAGATG
    TTTGGGGCCT GCCTAACCAC AACAGAGCTG CCCACAGGTG TGCTGTCCAC GGAAGACCTC
    CTCATGTCCC ACACAAGGCA GCAGGACAAG CTGCAGGATG AGCTGAAATT ACTTGGAAAG
    CAAGGGACCA CATTGCTGTC GTGTATTCAA GAACCAGCGA CCAAAAGTTC CACCAGCAAA
    CTCAATCCCA ATGAACTAGA GAATGTAGCT ACCATTGAAA GGTTATTACT TCAGTTGGAT
    GAGACAGAAA AAGCCTTTAA TCAGTTTTGG TCTGAGCATC ACTCAAAGCT TAACCAATGC
    CTACAACTAC AACACTTTGA GCACAATTTT TGTGAGGTTA AGCTTGCCCT GGACAATTTG
    TTGGCAGAGC AAGCGGAGTT TACAGACATT GGAGACAGTG TGATTCGTGT GGAGCAAATA
    CTTAAGGAAC ACAAAAAACT GGAAGAAAAA GGCCAGGAGC CCTTGGAAAA GGCCCAGCTG
    CTTGCAATAA TTGGGGACCA GCTCATCCAA AGCCACCACT ATGCAATGGA TTCCATCAGA
    CCGAGATGTG TAGAGCTCAG GCACCTCTGT GATGACTTCA TCAATGAAAA CAAGAAAAAA
    CATGACATTT TACAAAAGTC TTTGGAGCTG CATAGACAGC TGGACAAGGT CAGCCAGTGG
    TGTGAAACAG GAATCTACCT TTTGGCTTCC CAAGCTGTAG ACAAGTGCCA GTCACGAGAG
    GGGGTTGACG TTGCCTTGAA TGACATCGAG GCATTCCTGA GTACGGCGAA GGAGTACCAG
    TTGCCCAGCC ACATGGAATT TTACAGCCAG TTTGAGTTGA TACTCACCAT TGATGTAAAG
    GCCAAAGCCC AGGAAGTACT GCAGAAGCTG TGCGATGTAC AGGAAATATT TCACAAGAGG
    CAAATGAGTT TGATGAAACT GGCAGCCAGA CAGACTCGCC CAGTGCAACC TGTGGCCCCT
    CATCCTGAGT CCTCCCCAAA ATGGATGTCA CCAAAAACCA GCCAGCCCTT CACCTTGGCT
    CCTCTTCAGA GAACATCTGA GGAAACTTAT ACAGGGACAT TAGAAAAGGA AGATGATGAG
    ACTAAATTTG AAGTCAAGAA TGAAGAAATA CTGGAAAGCA GTCATGATGG GGAGAACCCT
    GGACAAGAGC AGCTTGAGAG TCCTGAAAAT CTTTCCCCTA CCAGGCGCAT TATACATGAC
    TTGCTTGAGA CGGAAGAGAT TTATATAAAA GAGATTAAGA GCATAATTGA TGGATATATC
    ACTCCAATGG ATTTTATTTG GCTGAAGCAT CTGATTCCAG ACGTTCTTCA GAATAACAAG
    GAGTTTCTCT TTGGGAATAT TAGAGAACTT TATGAATTTC ACAACAGGAC TTTTCTGAAA
    GAACTGGAAA AGTGCACTGA AAACCCCGAA ATGCTGGCAC GCTGCTTTCT CGAGAGAAAA
    GAAGATCTTC AAATATATTT TAAATACCAT AAGAATCTGC CCCGAGCTAG AGCAATCTGG
    CAAGAATGTC AAGACTGCAC CTTCTTTGGG GTTTGCCAGC GCCAACTGGA TCACAATCTC
    CCTCTTTTTA AGTATCTCAG AGGACCAAGC CAGAGACTTA TAAAATATCA GATGCTGCTG
    AAGGGTCTGC TGGATTTTGA GTCTCCTGAG GATTTGGGAA TAGACACAGG TGACCAGGAA
    GGAAGCTTGG CTAAGAATGG GCCAAAGAGA ACCAAGGATT CAGCTTTCTC AGCCGAACTA
    CAACAGGCTT TGGCCATGAT GGAAGATTTG ATCAAGTCCT GTGAATTGGC CGTGGACCTG
    GCAGCTGTCA CTGGATGTCC GGATGATATC AGGAAACTGG GAAAGCTACT GAAGCATGGC
    TCTTTCAACG TGTGGACAAT TCACAAGGAT CGTTATAAAA TGAAGGATTT TATTCGATTT
    AAGCCCAGAC AGAGGCAAAT CTATCTATTT GAAAGGGGAA TTGTATTCTG TAAGATACGA
    ATGGAGCCCA GTGACCAGGG CCTATCTCCT CATTACAGCT TTAAGAAATC TATGAAGTTG
    ATGACACTTT CAATTCGCCA GCTTGGAAGG GGGAGCAACC GGAAGTTTGA ACTTGCCAAC
    CGGAATGGAC TCGAGAAATA CATCCTGCAG GCAGCTTCGA AAGAAATCAG AGATTGTTGG
    TTTTCAGAAA TAAGTAAATT ACTGATGGAA CAACAAAACA ATATTAAAGA TATTTATGAA
    GATGAAGAAA ATGGCAAACA AATCTCCCCC AAATGCTGCT CCAGCAGCGA GTGTATCTCC
    ATGGAAACCT TTGAGGAGCG GAAAGGTGCA GAAGATATGG AAAAGGAGAG CAGTGCTCTG
    AGTCTATCCG GACTCTTCCA GTTGGACGAC AGTTACGAAA CGTGTTCCTC CAAACCTGTC
    CTGGCCACAG GAGAAAGCAT CCCGGGAAAA AGAGAAGGTG GAGACGAGGA CATGAAGCCT
    TCAGGCATCG CCACATTTCT CCTGGGGTGG GAGTGCGGAG ATCCGTTGAG AACCTCCTAT
    CGGAGCCTTG GTGGAAGCGG GTCGCTCTTC TCCCAGGATC CCCCCAAGAA TGTTGGCGTG
    GTGTTTGTTA CTTTGTTCCC GGATTCTATC TTTTCAAGTG GGAACTTCTT TCTTAAGCAC
    CCGAGGATTG TCTCCTGTGT GACTCAAGAA AATCACTTCA GAATTCAAGA AAACAAAAAT
    AAGCATCTCA CTTTATTCAT AAAGAAGCCC TTGACTTCGT GGCCAAAAAA AGGGCAGAGA
    CTAAGGGACA GACGATCCGG GACAGGCTCT TCAGCAAAAT TGGCCAACAG AGTGTTCTTT
    GAAATTTTTC AGAAAGTTCT GTTGTGGAGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe284494
    Clone ID Related Accession (Same CDS sequence) XM_022414336.1
    Accession Version XM_022414336.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3390bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product probable guanine nucleotide exchange factor MCF2L2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTATTTT GGTTAAAGGA AGAGATGGCC CCCCAGGAGC TGAGCCGGCG ACTGGCCACA 
    GTGATCACTC ATGTCGATGA AATTATGCAG CAGGAAATCA GGCCCTCAGT GGCAGTGGAT
    ATAATTGAAC AACTTCACAG ACAGTTTGCC ATTCTCTCAG GAGGTCGAGG GAAAGATGGT
    GCACCCATCA TCACTTTCCC AGAGTTTGTG GGGTTCAAAC ACATCCCAGA GGAAGACTTT
    CTGAATGTCA TGACCTACCT GACTAGCATC CCCAGTGTGG AGGCTGCCAG CATTGGATTC
    ATCATTGTTA TTGACCGACG AAGGGACAAG TGGAGCTCCA TAAAAGCATC ATTGACACGA
    ATAGCTATCA TTATGTTAAA CTCTGCATCT GACCTTCACG GCTACATTGA CAAAAACCAA
    CTGACAGAGG ATTTAGGGGG AACTTTGGAA TACCGCCACA GTCAGTGGAT AAATCATCGA
    ACTGCCATTG AAAATTTTGC CATGACATTG AAGACCACTG CCCAGATGCT TCAGATGTTT
    GGGGCCTGCC TAACCACAAC AGAGCTGCCC ACAGGTGTGC TGTCCACGGA AGACCTCCTC
    ATGTCCCACA CAAGGCAGCA GGACAAGCTG CAGGATGAGC TGAAATTACT TGGAAAGCAA
    GGGACCACAT TGCTGTCGTG TATTCAAGAA CCAGCGACCA AAAGTTCCAC CAGCAAACTC
    AATCCCAATG AACTAGAGAA TGTAGCTACC ATTGAAAGGT TATTACTTCA GTTGGATGAG
    ACAGAAAAAG CCTTTAATCA GTTTTGGTCT GAGCATCACT CAAAGCTTAA CCAATGCCTA
    CAACTACAAC ACTTTGAGCA CAATTTTTGT GAGGTTAAGC TTGCCCTGGA CAATTTGTTG
    GCAGAGCAAG CGGAGTTTAC AGACATTGGA GACAGTGTGA TTCGTGTGGA GCAAATACTT
    AAGGAACACA AAAAACTGGA AGAAAAAGGC CAGGAGCCCT TGGAAAAGGC CCAGCTGCTT
    GCAATAATTG GGGACCAGCT CATCCAAAGC CACCACTATG CAATGGATTC CATCAGACCG
    AGATGTGTAG AGCTCAGGCA CCTCTGTGAT GACTTCATCA ATGAAAACAA GAAAAAACAT
    GACATTTTAC AAAAGTCTTT GGAGCTGCAT AGACAGCTGG ACAAGGTCAG CCAGTGGTGT
    GAAACAGGAA TCTACCTTTT GGCTTCCCAA GCTGTAGACA AGTGCCAGTC ACGAGAGGGG
    GTTGACGTTG CCTTGAATGA CATCGAGGCA TTCCTGAGTA CGGCGAAGGA GTACCAGTTG
    CCCAGCCACA TGGAATTTTA CAGCCAGTTT GAGTTGATAC TCACCATTGA TGTAAAGGCC
    AAAGCCCAGG AAGTACTGCA GAAGCTGTGC GATGTACAGG AAATATTTCA CAAGAGGCAA
    ATGAGTTTGA TGAAACTGGC AGCCAGACAG ACTCGCCCAG TGCAACCTGT GGCCCCTCAT
    CCTGAGTCCT CCCCAAAATG GATGTCACCA AAAACCAGCC AGCCCTTCAC CTTGGCTCCT
    CTTCAGAGAA CATCTGAGGA AACTTATACA GGGACATTAG AAAAGGAAGA TGATGAGACT
    AAATTTGAAG TCAAGAATGA AGAAATACTG GAAAGCAGTC ATGATGGGGA GAACCCTGGA
    CAAGAGCAGC TTGAGAGTCC TGAAAATCTT TCCCCTACCA GGCGCATTAT ACATGACTTG
    CTTGAGACGG AAGAGATTTA TATAAAAGAG ATTAAGAGCA TAATTGATGG ATATATCACT
    CCAATGGATT TTATTTGGCT GAAGCATCTG ATTCCAGACG TTCTTCAGAA TAACAAGGAG
    TTTCTCTTTG GGAATATTAG AGAACTTTAT GAATTTCACA ACAGGACTTT TCTGAAAGAA
    CTGGAAAAGT GCACTGAAAA CCCCGAAATG CTGGCACGCT GCTTTCTCGA GAGAAAAGAA
    GATCTTCAAA TATATTTTAA ATACCATAAG AATCTGCCCC GAGCTAGAGC AATCTGGCAA
    GAATGTCAAG ACTGCACCTT CTTTGGGGTT TGCCAGCGCC AACTGGATCA CAATCTCCCT
    CTTTTTAAGT ATCTCAGAGG ACCAAGCCAG AGACTTATAA AATATCAGAT GCTGCTGAAG
    GGTCTGCTGG ATTTTGAGTC TCCTGAGGAT TTGGGAATAG ACACAGGTGA CCAGGAAGGA
    AGCTTGGCTA AGAATGGGCC AAAGAGAACC AAGGATTCAG CTTTCTCAGC CGAACTACAA
    CAGGCTTTGG CCATGATGGA AGATTTGATC AAGTCCTGTG AATTGGCCGT GGACCTGGCA
    GCTGTCACTG GATGTCCGGA TGATATCAGG AAACTGGGAA AGCTACTGAA GCATGGCTCT
    TTCAACGTGT GGACAATTCA CAAGGATCGT TATAAAATGA AGGATTTTAT TCGATTTAAG
    CCCAGACAGA GGCAAATCTA TCTATTTGAA AGGGGAATTG TATTCTGTAA GATACGAATG
    GAGCCCAGTG ACCAGGGCCT ATCTCCTCAT TACAGCTTTA AGAAATCTAT GAAGTTGATG
    ACACTTTCAA TTCGCCAGCT TGGAAGGGGG AGCAACCGGA AGTTTGAACT TGCCAACCGG
    AATGGACTCG AGAAATACAT CCTGCAGGCA GCTTCGAAAG AAATCAGAGA TTGTTGGTTT
    TCAGAAATAA GTAAATTACT GATGGAACAA CAAAACAATA TTAAAGATAT TTATGAAGAT
    GAAGAAAATG GCAAACAAAT CTCCCCCAAA TGCTGCTCCA GCAGCGAGTG TATCTCCATG
    GAAACCTTTG AGGAGCGGAA AGGTGCAGAA GATATGGAAA AGGAGAGCAG TGCTCTGAGT
    CTATCCGGAC TCTTCCAGTT GGACGACAGT TACGAAACGT GTTCCTCCAA ACCTGTCCTG
    GCCACAGGAG AAAGCATCCC GGGAAAAAGA GAAGGTGGAG ACGAGGACAT GAAGCCTTCA
    GGCATCGCCA CATTTCTCCT GGGGTGGGAG TGCGGAGATC CGTTGAGAAC CTCCTATCGG
    AGCCTTGGTG GAAGCGGGTC GCTCTTCTCC CAGGATCCCC CCAAGAATGT TGGCGTGGTG
    TTTGTTACTT TGTTCCCGGA TTCTATCTTT TCAAGTGGGA ACTTCTTTCT TAAGCACCCG
    AGGATTGTCT CCTGTGTGAC TCAAGAAAAT CACTTCAGAA TTCAAGAAAA CAAAAATAAG
    CATCTCACTT TATTCATAAA GAAGCCCTTG ACTTCGTGGC CAAAAAAAGG GCAGAGACTA
    AGGGACAGAC GATCCGGGAC AGGCTCTTCA GCAAAATTGG CCAACAGAGT GTTCTTTGAA
    ATTTTTCAGA AAGTTCTGTT GTGGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022270044.1
    CDS436..3825
    Translation

    Target ORF information:

    RefSeq Version XM_022414336.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022414336.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTATTTT GGTTAAAGGA AGAGATGGCC CCCCAGGAGC TGAGCCGGCG ACTGGCCACA 
    GTGATCACTC ATGTCGATGA AATTATGCAG CAGGAAATCA GGCCCTCAGT GGCAGTGGAT
    ATAATTGAAC AACTTCACAG ACAGTTTGCC ATTCTCTCAG GAGGTCGAGG GAAAGATGGT
    GCACCCATCA TCACTTTCCC AGAGTTTGTG GGGTTCAAAC ACATCCCAGA GGAAGACTTT
    CTGAATGTCA TGACCTACCT GACTAGCATC CCCAGTGTGG AGGCTGCCAG CATTGGATTC
    ATCATTGTTA TTGACCGACG AAGGGACAAG TGGAGCTCCA TAAAAGCATC ATTGACACGA
    ATAGCTATCA TTATGTTAAA CTCTGCATCT GACCTTCACG GCTACATTGA CAAAAACCAA
    CTGACAGAGG ATTTAGGGGG AACTTTGGAA TACCGCCACA GTCAGTGGAT AAATCATCGA
    ACTGCCATTG AAAATTTTGC CATGACATTG AAGACCACTG CCCAGATGCT TCAGATGTTT
    GGGGCCTGCC TAACCACAAC AGAGCTGCCC ACAGGTGTGC TGTCCACGGA AGACCTCCTC
    ATGTCCCACA CAAGGCAGCA GGACAAGCTG CAGGATGAGC TGAAATTACT TGGAAAGCAA
    GGGACCACAT TGCTGTCGTG TATTCAAGAA CCAGCGACCA AAAGTTCCAC CAGCAAACTC
    AATCCCAATG AACTAGAGAA TGTAGCTACC ATTGAAAGGT TATTACTTCA GTTGGATGAG
    ACAGAAAAAG CCTTTAATCA GTTTTGGTCT GAGCATCACT CAAAGCTTAA CCAATGCCTA
    CAACTACAAC ACTTTGAGCA CAATTTTTGT GAGGTTAAGC TTGCCCTGGA CAATTTGTTG
    GCAGAGCAAG CGGAGTTTAC AGACATTGGA GACAGTGTGA TTCGTGTGGA GCAAATACTT
    AAGGAACACA AAAAACTGGA AGAAAAAGGC CAGGAGCCCT TGGAAAAGGC CCAGCTGCTT
    GCAATAATTG GGGACCAGCT CATCCAAAGC CACCACTATG CAATGGATTC CATCAGACCG
    AGATGTGTAG AGCTCAGGCA CCTCTGTGAT GACTTCATCA ATGAAAACAA GAAAAAACAT
    GACATTTTAC AAAAGTCTTT GGAGCTGCAT AGACAGCTGG ACAAGGTCAG CCAGTGGTGT
    GAAACAGGAA TCTACCTTTT GGCTTCCCAA GCTGTAGACA AGTGCCAGTC ACGAGAGGGG
    GTTGACGTTG CCTTGAATGA CATCGAGGCA TTCCTGAGTA CGGCGAAGGA GTACCAGTTG
    CCCAGCCACA TGGAATTTTA CAGCCAGTTT GAGTTGATAC TCACCATTGA TGTAAAGGCC
    AAAGCCCAGG AAGTACTGCA GAAGCTGTGC GATGTACAGG AAATATTTCA CAAGAGGCAA
    ATGAGTTTGA TGAAACTGGC AGCCAGACAG ACTCGCCCAG TGCAACCTGT GGCCCCTCAT
    CCTGAGTCCT CCCCAAAATG GATGTCACCA AAAACCAGCC AGCCCTTCAC CTTGGCTCCT
    CTTCAGAGAA CATCTGAGGA AACTTATACA GGGACATTAG AAAAGGAAGA TGATGAGACT
    AAATTTGAAG TCAAGAATGA AGAAATACTG GAAAGCAGTC ATGATGGGGA GAACCCTGGA
    CAAGAGCAGC TTGAGAGTCC TGAAAATCTT TCCCCTACCA GGCGCATTAT ACATGACTTG
    CTTGAGACGG AAGAGATTTA TATAAAAGAG ATTAAGAGCA TAATTGATGG ATATATCACT
    CCAATGGATT TTATTTGGCT GAAGCATCTG ATTCCAGACG TTCTTCAGAA TAACAAGGAG
    TTTCTCTTTG GGAATATTAG AGAACTTTAT GAATTTCACA ACAGGACTTT TCTGAAAGAA
    CTGGAAAAGT GCACTGAAAA CCCCGAAATG CTGGCACGCT GCTTTCTCGA GAGAAAAGAA
    GATCTTCAAA TATATTTTAA ATACCATAAG AATCTGCCCC GAGCTAGAGC AATCTGGCAA
    GAATGTCAAG ACTGCACCTT CTTTGGGGTT TGCCAGCGCC AACTGGATCA CAATCTCCCT
    CTTTTTAAGT ATCTCAGAGG ACCAAGCCAG AGACTTATAA AATATCAGAT GCTGCTGAAG
    GGTCTGCTGG ATTTTGAGTC TCCTGAGGAT TTGGGAATAG ACACAGGTGA CCAGGAAGGA
    AGCTTGGCTA AGAATGGGCC AAAGAGAACC AAGGATTCAG CTTTCTCAGC CGAACTACAA
    CAGGCTTTGG CCATGATGGA AGATTTGATC AAGTCCTGTG AATTGGCCGT GGACCTGGCA
    GCTGTCACTG GATGTCCGGA TGATATCAGG AAACTGGGAA AGCTACTGAA GCATGGCTCT
    TTCAACGTGT GGACAATTCA CAAGGATCGT TATAAAATGA AGGATTTTAT TCGATTTAAG
    CCCAGACAGA GGCAAATCTA TCTATTTGAA AGGGGAATTG TATTCTGTAA GATACGAATG
    GAGCCCAGTG ACCAGGGCCT ATCTCCTCAT TACAGCTTTA AGAAATCTAT GAAGTTGATG
    ACACTTTCAA TTCGCCAGCT TGGAAGGGGG AGCAACCGGA AGTTTGAACT TGCCAACCGG
    AATGGACTCG AGAAATACAT CCTGCAGGCA GCTTCGAAAG AAATCAGAGA TTGTTGGTTT
    TCAGAAATAA GTAAATTACT GATGGAACAA CAAAACAATA TTAAAGATAT TTATGAAGAT
    GAAGAAAATG GCAAACAAAT CTCCCCCAAA TGCTGCTCCA GCAGCGAGTG TATCTCCATG
    GAAACCTTTG AGGAGCGGAA AGGTGCAGAA GATATGGAAA AGGAGAGCAG TGCTCTGAGT
    CTATCCGGAC TCTTCCAGTT GGACGACAGT TACGAAACGT GTTCCTCCAA ACCTGTCCTG
    GCCACAGGAG AAAGCATCCC GGGAAAAAGA GAAGGTGGAG ACGAGGACAT GAAGCCTTCA
    GGCATCGCCA CATTTCTCCT GGGGTGGGAG TGCGGAGATC CGTTGAGAAC CTCCTATCGG
    AGCCTTGGTG GAAGCGGGTC GCTCTTCTCC CAGGATCCCC CCAAGAATGT TGGCGTGGTG
    TTTGTTACTT TGTTCCCGGA TTCTATCTTT TCAAGTGGGA ACTTCTTTCT TAAGCACCCG
    AGGATTGTCT CCTGTGTGAC TCAAGAAAAT CACTTCAGAA TTCAAGAAAA CAAAAATAAG
    CATCTCACTT TATTCATAAA GAAGCCCTTG ACTTCGTGGC CAAAAAAAGG GCAGAGACTA
    AGGGACAGAC GATCCGGGAC AGGCTCTTCA GCAAAATTGG CCAACAGAGT GTTCTTTGAA
    ATTTTTCAGA AAGTTCTGTT GTGGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe284495
    Clone ID Related Accession (Same CDS sequence) XM_022414337.1
    Accession Version XM_022414337.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3300bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product probable guanine nucleotide exchange factor MCF2L2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTATTTT GGTTAAAGGA AGAGATGGCC CCCCAGGAGC TGAGCCGGCG ACTGGCCACA 
    GTGATCACTC ATGTCGATGA AATTATGCAG CAGGAAATCA GGCCCTCAGT GGCAGTGGAT
    ATAATTGAAC AACTTCACAG ACAGTTTGCC ATTCTCTCAG GAGGTCGAGG GAAAGATGGT
    GCACCCATCA TCACTTTCCC AGAGTTTGTG GGGTTCAAAC ACATCCCAGA GGAAGACTTT
    CTGAATGTCA TGACCTACCT GACTAGCATC CCCAGTGTGG AGGCTGCCAG CATTGGATTC
    ATCATTGTTA TTGACCGACG AAGGGACAAG TGGAGCTCCA TAAAAGCATC ATTGACACGA
    ATAGCTGTAG CATTTCCAGG AAACTTGCAG CTCATATTCA TCCTTCGTCC ATCTCGCTTT
    ATCCAGAGGA CATTCACTGA CATTGGCATT AAATATTATC GAGATGAATT TAAAATGAAA
    GTTCCGATCA TTATGTTAAA CTCTGCATCT GACCTTCACG GCTACATTGA CAAAAACCAA
    CTGACAGAGG ATTTAGGGGG AACTTTGGAA TACCGCCACA GTCAGTGGAT AAATCATCGA
    ACTGCCATTG AAAATTTTGC CATGACATTG AAGACCACTG CCCAGATGCT TCAGATGTTT
    GGGGCCTGCC TAACCACAAC AGAGCTGCCC ACAGGTGTGC TGTCCACGGA AGACCTCCTC
    ATGTCCCACA CAAGGCAGCA GGACAAGCTG CAGGATGAGC TGAAATTACT TGGAAAGCAA
    GGGACCACAT TGCTGTCGTG TATTCAAGAA CCAGCGACCA AAAGTTCCAC CAGCAAACTC
    AATCCCAATG AACTAGAGAA TGTAGCTACC ATTGAAAGGT TATTACTTCA GTTGGATGAG
    ACAGAAAAAG CCTTTAATCA GTTTTGGTCT GAGCATCACT CAAAGCTTAA CCAATGCCTA
    CAACTACAAC ACTTTGAGCA CAATTTTTGT GAGGTTAAGC TTGCCCTGGA CAATTTGTTG
    GCAGAGCAAG CGGAGTTTAC AGACATTGGA GACAGTGTGA TTCGTGTGGA GCAAATACTT
    AAGGAACACA AAAAACTGGA AGAAAAAGGC CAGGAGCCCT TGGAAAAGGC CCAGCTGCTT
    GCAATAATTG GGGACCAGCT CATCCAAAGC CACCACTATG CAATGGATTC CATCAGACCG
    AGATGTGTAG AGCTCAGGCA CCTCTGTGAT GACTTCATCA ATGAAAACAA GAAAAAACAT
    GACATTTTAC AAAAGTCTTT GGAGCTGCAT AGACAGCTGG ACAAGGTCAG CCAGTGGTGT
    GAAACAGGAA TCTACCTTTT GGCTTCCCAA GCTGTAGACA AGTGCCAGTC ACGAGAGGGG
    GTTGACGTTG CCTTGAATGA CATCGAGGCA TTCCTGAGTA CGGCGAAGGA GTACCAGTTG
    CCCAGCCACA TGGAATTTTA CAGCCAGTTT GAGTTGATAC TCACCATTGA TGTAAAGGCC
    AAAGCCCAGG AAGTACTGCA GAAGCTGTGC GATGTACAGG AAATATTTCA CAAGAGGCAA
    ATGAGTTTGA TGAAACTGGC AGCCAGACAG ACTCGCCCAG TGCAACCTGT GGCCCCTCAT
    CCTGAGTCCT CCCCAAAATG GATGTCACCA AAAACCAGCC AGCCCTTCAC CTTGGCTCCT
    CTTCAGAGAA CATCTGAGGA AACTTATACA GGGACATTAG AAAAGGAAGA TGATGAGACT
    AAATTTGAAG TCAAGAATGA AGAAATACTG GAAAGCAGTC ATGATGGGGA GAACCCTGGA
    CAAGAGCAGC TTGAGAGTCC TGAAAATCTT TCCCCTACCA GGCGCATTAT ACATGACTTG
    CTTGAGACGG AAGAGATTTA TATAAAAGAG ATTAAGAGCA TAATTGATGG ATATATCACT
    CCAATGGATT TTATTTGGCT GAAGCATCTG ATTCCAGACG TTCTTCAGAA TAACAAGGAG
    TTTCTCTTTG GGAATATTAG AGAACTTTAT GAATTTCACA ACAGGACTTT TCTGAAAGAA
    CTGGAAAAGT GCACTGAAAA CCCCGAAATG CTGGCACGCT GCTTTCTCGA GAGAAAAGAA
    GATCTTCAAA TATATTTTAA ATACCATAAG AATCTGCCCC GAGCTAGAGC AATCTGGCAA
    GAATGTCAAG ACTGCACCTT CTTTGGGGTT TGCCAGCGCC AACTGGATCA CAATCTCCCT
    CTTTTTAAGT ATCTCAGAGG ACCAAGCCAG AGACTTATAA AATATCAGAT GCTGCTGAAG
    GGTCTGCTGG ATTTTGAGTC TCCTGAGGAT TTGGGAATAG ACACAGGTGA CCAGGAAGGA
    AGCTTGGCTA AGAATGGGCC AAAGAGAACC AAGGATTCAG CTTTCTCAGC CGAACTACAA
    CAGGCTTTGG CCATGATGGA AGATTTGATC AAGTCCTGTG AATTGGCCGT GGACCTGGCA
    GCTGTCACTG GATGTCCGGA TGATATCAGG AAACTGGGAA AGCTACTGAA GCATGGCTCT
    TTCAACGTGT GGACAATTCA CAAGGATCGT TATAAAATGA AGGATTTTAT TCGATTTAAG
    CCCAGACAGA GGCAAATCTA TCTATTTGAA AGGGGAATTG TATTCTGTAA GATACGAATG
    GAGCCCAGTG ACCAGGGCCT ATCTCCTCAT TACAGCTTTA AGAAATCTAT GAAGTTGATG
    ACACTTTCAA TTCGCCAGCT TGGAAGGGGG AGCAACCGGA AGTTTGAACT TGCCAACCGG
    AATGGACTCG AGAAATACAT CCTGCAGGCA GCTTCGAAAG AAATCAGAGA TTGTTGGTTT
    TCAGAAATAA GTAAATTACT GATGGAACAA CAAAACAATA TTAAAGATAT TTATGAAGAT
    GAAGAAAATG GCAAACAAAT CTCCCCCAAA TGCTGCTCCA GCAGCGAGTG TATCTCCATG
    GAAACCTTTG AGGAGCGGAA AGGTGCAGAA GATATGGAAA AGGAGAGCAG TGCTCTGAGT
    CTATCCGGAC TCTTCCAGTT GGACGACAGT TACGAAACGT GTTCCTCCAA ACCTGTCCTG
    GCCACAGGAG AAAGCATCCC GGGAAAAAGA GAAGGTGGAG ACGAGGACAT GAAGCCTTCA
    GGCATCGCCA CATTTCTCCT GGGGTGGGAG TGCGGAGATC CGTTGAGAAC CTCCTATCGG
    AGCCTTGGTG GAAGCGGGTC GCTCTTCTCC CAGGATCCCC CCAAGAATGT TGGCGTGGTG
    TTTGTTACTT TGTTCCCGGA TTCTATCTTT TCAAGCACCC GAGGATTGTC TCCTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022270045.1
    CDS437..3736
    Translation

    Target ORF information:

    RefSeq Version XM_022414337.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022414337.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTATTTT GGTTAAAGGA AGAGATGGCC CCCCAGGAGC TGAGCCGGCG ACTGGCCACA 
    GTGATCACTC ATGTCGATGA AATTATGCAG CAGGAAATCA GGCCCTCAGT GGCAGTGGAT
    ATAATTGAAC AACTTCACAG ACAGTTTGCC ATTCTCTCAG GAGGTCGAGG GAAAGATGGT
    GCACCCATCA TCACTTTCCC AGAGTTTGTG GGGTTCAAAC ACATCCCAGA GGAAGACTTT
    CTGAATGTCA TGACCTACCT GACTAGCATC CCCAGTGTGG AGGCTGCCAG CATTGGATTC
    ATCATTGTTA TTGACCGACG AAGGGACAAG TGGAGCTCCA TAAAAGCATC ATTGACACGA
    ATAGCTGTAG CATTTCCAGG AAACTTGCAG CTCATATTCA TCCTTCGTCC ATCTCGCTTT
    ATCCAGAGGA CATTCACTGA CATTGGCATT AAATATTATC GAGATGAATT TAAAATGAAA
    GTTCCGATCA TTATGTTAAA CTCTGCATCT GACCTTCACG GCTACATTGA CAAAAACCAA
    CTGACAGAGG ATTTAGGGGG AACTTTGGAA TACCGCCACA GTCAGTGGAT AAATCATCGA
    ACTGCCATTG AAAATTTTGC CATGACATTG AAGACCACTG CCCAGATGCT TCAGATGTTT
    GGGGCCTGCC TAACCACAAC AGAGCTGCCC ACAGGTGTGC TGTCCACGGA AGACCTCCTC
    ATGTCCCACA CAAGGCAGCA GGACAAGCTG CAGGATGAGC TGAAATTACT TGGAAAGCAA
    GGGACCACAT TGCTGTCGTG TATTCAAGAA CCAGCGACCA AAAGTTCCAC CAGCAAACTC
    AATCCCAATG AACTAGAGAA TGTAGCTACC ATTGAAAGGT TATTACTTCA GTTGGATGAG
    ACAGAAAAAG CCTTTAATCA GTTTTGGTCT GAGCATCACT CAAAGCTTAA CCAATGCCTA
    CAACTACAAC ACTTTGAGCA CAATTTTTGT GAGGTTAAGC TTGCCCTGGA CAATTTGTTG
    GCAGAGCAAG CGGAGTTTAC AGACATTGGA GACAGTGTGA TTCGTGTGGA GCAAATACTT
    AAGGAACACA AAAAACTGGA AGAAAAAGGC CAGGAGCCCT TGGAAAAGGC CCAGCTGCTT
    GCAATAATTG GGGACCAGCT CATCCAAAGC CACCACTATG CAATGGATTC CATCAGACCG
    AGATGTGTAG AGCTCAGGCA CCTCTGTGAT GACTTCATCA ATGAAAACAA GAAAAAACAT
    GACATTTTAC AAAAGTCTTT GGAGCTGCAT AGACAGCTGG ACAAGGTCAG CCAGTGGTGT
    GAAACAGGAA TCTACCTTTT GGCTTCCCAA GCTGTAGACA AGTGCCAGTC ACGAGAGGGG
    GTTGACGTTG CCTTGAATGA CATCGAGGCA TTCCTGAGTA CGGCGAAGGA GTACCAGTTG
    CCCAGCCACA TGGAATTTTA CAGCCAGTTT GAGTTGATAC TCACCATTGA TGTAAAGGCC
    AAAGCCCAGG AAGTACTGCA GAAGCTGTGC GATGTACAGG AAATATTTCA CAAGAGGCAA
    ATGAGTTTGA TGAAACTGGC AGCCAGACAG ACTCGCCCAG TGCAACCTGT GGCCCCTCAT
    CCTGAGTCCT CCCCAAAATG GATGTCACCA AAAACCAGCC AGCCCTTCAC CTTGGCTCCT
    CTTCAGAGAA CATCTGAGGA AACTTATACA GGGACATTAG AAAAGGAAGA TGATGAGACT
    AAATTTGAAG TCAAGAATGA AGAAATACTG GAAAGCAGTC ATGATGGGGA GAACCCTGGA
    CAAGAGCAGC TTGAGAGTCC TGAAAATCTT TCCCCTACCA GGCGCATTAT ACATGACTTG
    CTTGAGACGG AAGAGATTTA TATAAAAGAG ATTAAGAGCA TAATTGATGG ATATATCACT
    CCAATGGATT TTATTTGGCT GAAGCATCTG ATTCCAGACG TTCTTCAGAA TAACAAGGAG
    TTTCTCTTTG GGAATATTAG AGAACTTTAT GAATTTCACA ACAGGACTTT TCTGAAAGAA
    CTGGAAAAGT GCACTGAAAA CCCCGAAATG CTGGCACGCT GCTTTCTCGA GAGAAAAGAA
    GATCTTCAAA TATATTTTAA ATACCATAAG AATCTGCCCC GAGCTAGAGC AATCTGGCAA
    GAATGTCAAG ACTGCACCTT CTTTGGGGTT TGCCAGCGCC AACTGGATCA CAATCTCCCT
    CTTTTTAAGT ATCTCAGAGG ACCAAGCCAG AGACTTATAA AATATCAGAT GCTGCTGAAG
    GGTCTGCTGG ATTTTGAGTC TCCTGAGGAT TTGGGAATAG ACACAGGTGA CCAGGAAGGA
    AGCTTGGCTA AGAATGGGCC AAAGAGAACC AAGGATTCAG CTTTCTCAGC CGAACTACAA
    CAGGCTTTGG CCATGATGGA AGATTTGATC AAGTCCTGTG AATTGGCCGT GGACCTGGCA
    GCTGTCACTG GATGTCCGGA TGATATCAGG AAACTGGGAA AGCTACTGAA GCATGGCTCT
    TTCAACGTGT GGACAATTCA CAAGGATCGT TATAAAATGA AGGATTTTAT TCGATTTAAG
    CCCAGACAGA GGCAAATCTA TCTATTTGAA AGGGGAATTG TATTCTGTAA GATACGAATG
    GAGCCCAGTG ACCAGGGCCT ATCTCCTCAT TACAGCTTTA AGAAATCTAT GAAGTTGATG
    ACACTTTCAA TTCGCCAGCT TGGAAGGGGG AGCAACCGGA AGTTTGAACT TGCCAACCGG
    AATGGACTCG AGAAATACAT CCTGCAGGCA GCTTCGAAAG AAATCAGAGA TTGTTGGTTT
    TCAGAAATAA GTAAATTACT GATGGAACAA CAAAACAATA TTAAAGATAT TTATGAAGAT
    GAAGAAAATG GCAAACAAAT CTCCCCCAAA TGCTGCTCCA GCAGCGAGTG TATCTCCATG
    GAAACCTTTG AGGAGCGGAA AGGTGCAGAA GATATGGAAA AGGAGAGCAG TGCTCTGAGT
    CTATCCGGAC TCTTCCAGTT GGACGACAGT TACGAAACGT GTTCCTCCAA ACCTGTCCTG
    GCCACAGGAG AAAGCATCCC GGGAAAAAGA GAAGGTGGAG ACGAGGACAT GAAGCCTTCA
    GGCATCGCCA CATTTCTCCT GGGGTGGGAG TGCGGAGATC CGTTGAGAAC CTCCTATCGG
    AGCCTTGGTG GAAGCGGGTC GCTCTTCTCC CAGGATCCCC CCAAGAATGT TGGCGTGGTG
    TTTGTTACTT TGTTCCCGGA TTCTATCTTT TCAAGCACCC GAGGATTGTC TCCTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe284496
    Clone ID Related Accession (Same CDS sequence) XM_022414339.1 , XM_022414338.1 , XM_022414340.1
    Accession Version XM_022414339.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3036bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product probable guanine nucleotide exchange factor MCF2L2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAAAGTTC CGATCATTAT GTTAAACTCT GCATCTGACC TTCACGGCTA CATTGACAAA 
    AACCAACTGA CAGAGGATTT AGGGGGAACT TTGGAATACC GCCACAGTCA GTGGATAAAT
    CATCGAACTG CCATTGAAAA TTTTGCCATG ACATTGAAGA CCACTGCCCA GATGCTTCAG
    ATGTTTGGGG CCTGCCTAAC CACAACAGAG CTGCCCACAG GTGTGCTGTC CACGGAAGAC
    CTCCTCATGT CCCACACAAG GCAGCAGGAC AAGCTGCAGG ATGAGCTGAA ATTACTTGGA
    AAGCAAGGGA CCACATTGCT GTCGTGTATT CAAGAACCAG CGACCAAAAG TTCCACCAGC
    AAACTCAATC CCAATGAACT AGAGAATGTA GCTACCATTG AAAGGTTATT ACTTCAGTTG
    GATGAGACAG AAAAAGCCTT TAATCAGTTT TGGTCTGAGC ATCACTCAAA GCTTAACCAA
    TGCCTACAAC TACAACACTT TGAGCACAAT TTTTGTGAGG TTAAGCTTGC CCTGGACAAT
    TTGTTGGCAG AGCAAGCGGA GTTTACAGAC ATTGGAGACA GTGTGATTCG TGTGGAGCAA
    ATACTTAAGG AACACAAAAA ACTGGAAGAA AAAGGCCAGG AGCCCTTGGA AAAGGCCCAG
    CTGCTTGCAA TAATTGGGGA CCAGCTCATC CAAAGCCACC ACTATGCAAT GGATTCCATC
    AGACCGAGAT GTGTAGAGCT CAGGCACCTC TGTGATGACT TCATCAATGA AAACAAGAAA
    AAACATGACA TTTTACAAAA GTCTTTGGAG CTGCATAGAC AGCTGGACAA GGTCAGCCAG
    TGGTGTGAAA CAGGAATCTA CCTTTTGGCT TCCCAAGCTG TAGACAAGTG CCAGTCACGA
    GAGGGGGTTG ACGTTGCCTT GAATGACATC GAGGCATTCC TGAGTACGGC GAAGGAGTAC
    CAGTTGCCCA GCCACATGGA ATTTTACAGC CAGTTTGAGT TGATACTCAC CATTGATGTA
    AAGGCCAAAG CCCAGGAAGT ACTGCAGAAG CTGTGCGATG TACAGGAAAT ATTTCACAAG
    AGGCAAATGA GTTTGATGAA ACTGGCAGCC AGACAGACTC GCCCAGTGCA ACCTGTGGCC
    CCTCATCCTG AGTCCTCCCC AAAATGGATG TCACCAAAAA CCAGCCAGCC CTTCACCTTG
    GCTCCTCTTC AGAGAACATC TGAGGAAACT TATACAGGGA CATTAGAAAA GGAAGATGAT
    GAGACTAAAT TTGAAGTCAA GAATGAAGAA ATACTGGAAA GCAGTCATGA TGGGGAGAAC
    CCTGGACAAG AGCAGCTTGA GAGTCCTGAA AATCTTTCCC CTACCAGGCG CATTATACAT
    GACTTGCTTG AGACGGAAGA GATTTATATA AAAGAGATTA AGAGCATAAT TGATGGATAT
    ATCACTCCAA TGGATTTTAT TTGGCTGAAG CATCTGATTC CAGACGTTCT TCAGAATAAC
    AAGGAGTTTC TCTTTGGGAA TATTAGAGAA CTTTATGAAT TTCACAACAG GACTTTTCTG
    AAAGAACTGG AAAAGTGCAC TGAAAACCCC GAAATGCTGG CACGCTGCTT TCTCGAGAGA
    AAAGAAGATC TTCAAATATA TTTTAAATAC CATAAGAATC TGCCCCGAGC TAGAGCAATC
    TGGCAAGAAT GTCAAGACTG CACCTTCTTT GGGGTTTGCC AGCGCCAACT GGATCACAAT
    CTCCCTCTTT TTAAGTATCT CAGAGGACCA AGCCAGAGAC TTATAAAATA TCAGATGCTG
    CTGAAGGGTC TGCTGGATTT TGAGTCTCCT GAGGATTTGG GAATAGACAC AGGTGACCAG
    GAAGGAAGCT TGGCTAAGAA TGGGCCAAAG AGAACCAAGG ATTCAGCTTT CTCAGCCGAA
    CTACAACAGG CTTTGGCCAT GATGGAAGAT TTGATCAAGT CCTGTGAATT GGCCGTGGAC
    CTGGCAGCTG TCACTGGATG TCCGGATGAT ATCAGGAAAC TGGGAAAGCT ACTGAAGCAT
    GGCTCTTTCA ACGTGTGGAC AATTCACAAG GATCGTTATA AAATGAAGGA TTTTATTCGA
    TTTAAGCCCA GACAGAGGCA AATCTATCTA TTTGAAAGGG GAATTGTATT CTGTAAGATA
    CGAATGGAGC CCAGTGACCA GGGCCTATCT CCTCATTACA GCTTTAAGAA ATCTATGAAG
    TTGATGACAC TTTCAATTCG CCAGCTTGGA AGGGGGAGCA ACCGGAAGTT TGAACTTGCC
    AACCGGAATG GACTCGAGAA ATACATCCTG CAGGCAGCTT CGAAAGAAAT CAGAGATTGT
    TGGTTTTCAG AAATAAGTAA ATTACTGATG GAACAACAAA ACAATATTAA AGATATTTAT
    GAAGATGAAG AAAATGGCAA ACAAATCTCC CCCAAATGCT GCTCCAGCAG CGAGTGTATC
    TCCATGGAAA CCTTTGAGGA GCGGAAAGGT GCAGAAGATA TGGAAAAGGA GAGCAGTGCT
    CTGAGTCTAT CCGGACTCTT CCAGTTGGAC GACAGTTACG AAACGTGTTC CTCCAAACCT
    GTCCTGGCCA CAGGAGAAAG CATCCCGGGA AAAAGAGAAG GTGGAGACGA GGACATGAAG
    CCTTCAGGCA TCGCCACATT TCTCCTGGGG TGGGAGTGCG GAGATCCGTT GAGAACCTCC
    TATCGGAGCC TTGGTGGAAG CGGGTCGCTC TTCTCCCAGG ATCCCCCCAA GAATGTTGGC
    GTGGTGTTTG TTACTTTGTT CCCGGATTCT ATCTTTTCAA GTGGGAACTT CTTTCTTAAG
    CACCCGAGGA TTGTCTCCTG TGTGACTCAA GAAAATCACT TCAGAATTCA AGAAAACAAA
    AATAAGCATC TCACTTTATT CATAAAGAAG CCCTTGACTT CGTGGCCAAA AAAAGGGCAG
    AGACTAAGGG ACAGACGATC CGGGACAGGC TCTTCAGCAA AATTGGCCAA CAGAGTGTTC
    TTTGAAATTT TTCAGAAAGT TCTGTTGTGG AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022270047.1
    CDS326..3361
    Translation

    Target ORF information:

    RefSeq Version XM_022414339.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022414339.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAAAGTTC CGATCATTAT GTTAAACTCT GCATCTGACC TTCACGGCTA CATTGACAAA 
    AACCAACTGA CAGAGGATTT AGGGGGAACT TTGGAATACC GCCACAGTCA GTGGATAAAT
    CATCGAACTG CCATTGAAAA TTTTGCCATG ACATTGAAGA CCACTGCCCA GATGCTTCAG
    ATGTTTGGGG CCTGCCTAAC CACAACAGAG CTGCCCACAG GTGTGCTGTC CACGGAAGAC
    CTCCTCATGT CCCACACAAG GCAGCAGGAC AAGCTGCAGG ATGAGCTGAA ATTACTTGGA
    AAGCAAGGGA CCACATTGCT GTCGTGTATT CAAGAACCAG CGACCAAAAG TTCCACCAGC
    AAACTCAATC CCAATGAACT AGAGAATGTA GCTACCATTG AAAGGTTATT ACTTCAGTTG
    GATGAGACAG AAAAAGCCTT TAATCAGTTT TGGTCTGAGC ATCACTCAAA GCTTAACCAA
    TGCCTACAAC TACAACACTT TGAGCACAAT TTTTGTGAGG TTAAGCTTGC CCTGGACAAT
    TTGTTGGCAG AGCAAGCGGA GTTTACAGAC ATTGGAGACA GTGTGATTCG TGTGGAGCAA
    ATACTTAAGG AACACAAAAA ACTGGAAGAA AAAGGCCAGG AGCCCTTGGA AAAGGCCCAG
    CTGCTTGCAA TAATTGGGGA CCAGCTCATC CAAAGCCACC ACTATGCAAT GGATTCCATC
    AGACCGAGAT GTGTAGAGCT CAGGCACCTC TGTGATGACT TCATCAATGA AAACAAGAAA
    AAACATGACA TTTTACAAAA GTCTTTGGAG CTGCATAGAC AGCTGGACAA GGTCAGCCAG
    TGGTGTGAAA CAGGAATCTA CCTTTTGGCT TCCCAAGCTG TAGACAAGTG CCAGTCACGA
    GAGGGGGTTG ACGTTGCCTT GAATGACATC GAGGCATTCC TGAGTACGGC GAAGGAGTAC
    CAGTTGCCCA GCCACATGGA ATTTTACAGC CAGTTTGAGT TGATACTCAC CATTGATGTA
    AAGGCCAAAG CCCAGGAAGT ACTGCAGAAG CTGTGCGATG TACAGGAAAT ATTTCACAAG
    AGGCAAATGA GTTTGATGAA ACTGGCAGCC AGACAGACTC GCCCAGTGCA ACCTGTGGCC
    CCTCATCCTG AGTCCTCCCC AAAATGGATG TCACCAAAAA CCAGCCAGCC CTTCACCTTG
    GCTCCTCTTC AGAGAACATC TGAGGAAACT TATACAGGGA CATTAGAAAA GGAAGATGAT
    GAGACTAAAT TTGAAGTCAA GAATGAAGAA ATACTGGAAA GCAGTCATGA TGGGGAGAAC
    CCTGGACAAG AGCAGCTTGA GAGTCCTGAA AATCTTTCCC CTACCAGGCG CATTATACAT
    GACTTGCTTG AGACGGAAGA GATTTATATA AAAGAGATTA AGAGCATAAT TGATGGATAT
    ATCACTCCAA TGGATTTTAT TTGGCTGAAG CATCTGATTC CAGACGTTCT TCAGAATAAC
    AAGGAGTTTC TCTTTGGGAA TATTAGAGAA CTTTATGAAT TTCACAACAG GACTTTTCTG
    AAAGAACTGG AAAAGTGCAC TGAAAACCCC GAAATGCTGG CACGCTGCTT TCTCGAGAGA
    AAAGAAGATC TTCAAATATA TTTTAAATAC CATAAGAATC TGCCCCGAGC TAGAGCAATC
    TGGCAAGAAT GTCAAGACTG CACCTTCTTT GGGGTTTGCC AGCGCCAACT GGATCACAAT
    CTCCCTCTTT TTAAGTATCT CAGAGGACCA AGCCAGAGAC TTATAAAATA TCAGATGCTG
    CTGAAGGGTC TGCTGGATTT TGAGTCTCCT GAGGATTTGG GAATAGACAC AGGTGACCAG
    GAAGGAAGCT TGGCTAAGAA TGGGCCAAAG AGAACCAAGG ATTCAGCTTT CTCAGCCGAA
    CTACAACAGG CTTTGGCCAT GATGGAAGAT TTGATCAAGT CCTGTGAATT GGCCGTGGAC
    CTGGCAGCTG TCACTGGATG TCCGGATGAT ATCAGGAAAC TGGGAAAGCT ACTGAAGCAT
    GGCTCTTTCA ACGTGTGGAC AATTCACAAG GATCGTTATA AAATGAAGGA TTTTATTCGA
    TTTAAGCCCA GACAGAGGCA AATCTATCTA TTTGAAAGGG GAATTGTATT CTGTAAGATA
    CGAATGGAGC CCAGTGACCA GGGCCTATCT CCTCATTACA GCTTTAAGAA ATCTATGAAG
    TTGATGACAC TTTCAATTCG CCAGCTTGGA AGGGGGAGCA ACCGGAAGTT TGAACTTGCC
    AACCGGAATG GACTCGAGAA ATACATCCTG CAGGCAGCTT CGAAAGAAAT CAGAGATTGT
    TGGTTTTCAG AAATAAGTAA ATTACTGATG GAACAACAAA ACAATATTAA AGATATTTAT
    GAAGATGAAG AAAATGGCAA ACAAATCTCC CCCAAATGCT GCTCCAGCAG CGAGTGTATC
    TCCATGGAAA CCTTTGAGGA GCGGAAAGGT GCAGAAGATA TGGAAAAGGA GAGCAGTGCT
    CTGAGTCTAT CCGGACTCTT CCAGTTGGAC GACAGTTACG AAACGTGTTC CTCCAAACCT
    GTCCTGGCCA CAGGAGAAAG CATCCCGGGA AAAAGAGAAG GTGGAGACGA GGACATGAAG
    CCTTCAGGCA TCGCCACATT TCTCCTGGGG TGGGAGTGCG GAGATCCGTT GAGAACCTCC
    TATCGGAGCC TTGGTGGAAG CGGGTCGCTC TTCTCCCAGG ATCCCCCCAA GAATGTTGGC
    GTGGTGTTTG TTACTTTGTT CCCGGATTCT ATCTTTTCAA GTGGGAACTT CTTTCTTAAG
    CACCCGAGGA TTGTCTCCTG TGTGACTCAA GAAAATCACT TCAGAATTCA AGAAAACAAA
    AATAAGCATC TCACTTTATT CATAAAGAAG CCCTTGACTT CGTGGCCAAA AAAAGGGCAG
    AGACTAAGGG ACAGACGATC CGGGACAGGC TCTTCAGCAA AATTGGCCAA CAGAGTGTTC
    TTTGAAATTT TTCAGAAAGT TCTGTTGTGG AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe284496
    Clone ID Related Accession (Same CDS sequence) XM_022414339.1 , XM_022414338.1 , XM_022414340.1
    Accession Version XM_022414338.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3036bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product probable guanine nucleotide exchange factor MCF2L2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAAAGTTC CGATCATTAT GTTAAACTCT GCATCTGACC TTCACGGCTA CATTGACAAA 
    AACCAACTGA CAGAGGATTT AGGGGGAACT TTGGAATACC GCCACAGTCA GTGGATAAAT
    CATCGAACTG CCATTGAAAA TTTTGCCATG ACATTGAAGA CCACTGCCCA GATGCTTCAG
    ATGTTTGGGG CCTGCCTAAC CACAACAGAG CTGCCCACAG GTGTGCTGTC CACGGAAGAC
    CTCCTCATGT CCCACACAAG GCAGCAGGAC AAGCTGCAGG ATGAGCTGAA ATTACTTGGA
    AAGCAAGGGA CCACATTGCT GTCGTGTATT CAAGAACCAG CGACCAAAAG TTCCACCAGC
    AAACTCAATC CCAATGAACT AGAGAATGTA GCTACCATTG AAAGGTTATT ACTTCAGTTG
    GATGAGACAG AAAAAGCCTT TAATCAGTTT TGGTCTGAGC ATCACTCAAA GCTTAACCAA
    TGCCTACAAC TACAACACTT TGAGCACAAT TTTTGTGAGG TTAAGCTTGC CCTGGACAAT
    TTGTTGGCAG AGCAAGCGGA GTTTACAGAC ATTGGAGACA GTGTGATTCG TGTGGAGCAA
    ATACTTAAGG AACACAAAAA ACTGGAAGAA AAAGGCCAGG AGCCCTTGGA AAAGGCCCAG
    CTGCTTGCAA TAATTGGGGA CCAGCTCATC CAAAGCCACC ACTATGCAAT GGATTCCATC
    AGACCGAGAT GTGTAGAGCT CAGGCACCTC TGTGATGACT TCATCAATGA AAACAAGAAA
    AAACATGACA TTTTACAAAA GTCTTTGGAG CTGCATAGAC AGCTGGACAA GGTCAGCCAG
    TGGTGTGAAA CAGGAATCTA CCTTTTGGCT TCCCAAGCTG TAGACAAGTG CCAGTCACGA
    GAGGGGGTTG ACGTTGCCTT GAATGACATC GAGGCATTCC TGAGTACGGC GAAGGAGTAC
    CAGTTGCCCA GCCACATGGA ATTTTACAGC CAGTTTGAGT TGATACTCAC CATTGATGTA
    AAGGCCAAAG CCCAGGAAGT ACTGCAGAAG CTGTGCGATG TACAGGAAAT ATTTCACAAG
    AGGCAAATGA GTTTGATGAA ACTGGCAGCC AGACAGACTC GCCCAGTGCA ACCTGTGGCC
    CCTCATCCTG AGTCCTCCCC AAAATGGATG TCACCAAAAA CCAGCCAGCC CTTCACCTTG
    GCTCCTCTTC AGAGAACATC TGAGGAAACT TATACAGGGA CATTAGAAAA GGAAGATGAT
    GAGACTAAAT TTGAAGTCAA GAATGAAGAA ATACTGGAAA GCAGTCATGA TGGGGAGAAC
    CCTGGACAAG AGCAGCTTGA GAGTCCTGAA AATCTTTCCC CTACCAGGCG CATTATACAT
    GACTTGCTTG AGACGGAAGA GATTTATATA AAAGAGATTA AGAGCATAAT TGATGGATAT
    ATCACTCCAA TGGATTTTAT TTGGCTGAAG CATCTGATTC CAGACGTTCT TCAGAATAAC
    AAGGAGTTTC TCTTTGGGAA TATTAGAGAA CTTTATGAAT TTCACAACAG GACTTTTCTG
    AAAGAACTGG AAAAGTGCAC TGAAAACCCC GAAATGCTGG CACGCTGCTT TCTCGAGAGA
    AAAGAAGATC TTCAAATATA TTTTAAATAC CATAAGAATC TGCCCCGAGC TAGAGCAATC
    TGGCAAGAAT GTCAAGACTG CACCTTCTTT GGGGTTTGCC AGCGCCAACT GGATCACAAT
    CTCCCTCTTT TTAAGTATCT CAGAGGACCA AGCCAGAGAC TTATAAAATA TCAGATGCTG
    CTGAAGGGTC TGCTGGATTT TGAGTCTCCT GAGGATTTGG GAATAGACAC AGGTGACCAG
    GAAGGAAGCT TGGCTAAGAA TGGGCCAAAG AGAACCAAGG ATTCAGCTTT CTCAGCCGAA
    CTACAACAGG CTTTGGCCAT GATGGAAGAT TTGATCAAGT CCTGTGAATT GGCCGTGGAC
    CTGGCAGCTG TCACTGGATG TCCGGATGAT ATCAGGAAAC TGGGAAAGCT ACTGAAGCAT
    GGCTCTTTCA ACGTGTGGAC AATTCACAAG GATCGTTATA AAATGAAGGA TTTTATTCGA
    TTTAAGCCCA GACAGAGGCA AATCTATCTA TTTGAAAGGG GAATTGTATT CTGTAAGATA
    CGAATGGAGC CCAGTGACCA GGGCCTATCT CCTCATTACA GCTTTAAGAA ATCTATGAAG
    TTGATGACAC TTTCAATTCG CCAGCTTGGA AGGGGGAGCA ACCGGAAGTT TGAACTTGCC
    AACCGGAATG GACTCGAGAA ATACATCCTG CAGGCAGCTT CGAAAGAAAT CAGAGATTGT
    TGGTTTTCAG AAATAAGTAA ATTACTGATG GAACAACAAA ACAATATTAA AGATATTTAT
    GAAGATGAAG AAAATGGCAA ACAAATCTCC CCCAAATGCT GCTCCAGCAG CGAGTGTATC
    TCCATGGAAA CCTTTGAGGA GCGGAAAGGT GCAGAAGATA TGGAAAAGGA GAGCAGTGCT
    CTGAGTCTAT CCGGACTCTT CCAGTTGGAC GACAGTTACG AAACGTGTTC CTCCAAACCT
    GTCCTGGCCA CAGGAGAAAG CATCCCGGGA AAAAGAGAAG GTGGAGACGA GGACATGAAG
    CCTTCAGGCA TCGCCACATT TCTCCTGGGG TGGGAGTGCG GAGATCCGTT GAGAACCTCC
    TATCGGAGCC TTGGTGGAAG CGGGTCGCTC TTCTCCCAGG ATCCCCCCAA GAATGTTGGC
    GTGGTGTTTG TTACTTTGTT CCCGGATTCT ATCTTTTCAA GTGGGAACTT CTTTCTTAAG
    CACCCGAGGA TTGTCTCCTG TGTGACTCAA GAAAATCACT TCAGAATTCA AGAAAACAAA
    AATAAGCATC TCACTTTATT CATAAAGAAG CCCTTGACTT CGTGGCCAAA AAAAGGGCAG
    AGACTAAGGG ACAGACGATC CGGGACAGGC TCTTCAGCAA AATTGGCCAA CAGAGTGTTC
    TTTGAAATTT TTCAGAAAGT TCTGTTGTGG AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022270046.1
    CDS284..3319
    Translation

    Target ORF information:

    RefSeq Version XM_022414338.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022414338.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAAAGTTC CGATCATTAT GTTAAACTCT GCATCTGACC TTCACGGCTA CATTGACAAA 
    AACCAACTGA CAGAGGATTT AGGGGGAACT TTGGAATACC GCCACAGTCA GTGGATAAAT
    CATCGAACTG CCATTGAAAA TTTTGCCATG ACATTGAAGA CCACTGCCCA GATGCTTCAG
    ATGTTTGGGG CCTGCCTAAC CACAACAGAG CTGCCCACAG GTGTGCTGTC CACGGAAGAC
    CTCCTCATGT CCCACACAAG GCAGCAGGAC AAGCTGCAGG ATGAGCTGAA ATTACTTGGA
    AAGCAAGGGA CCACATTGCT GTCGTGTATT CAAGAACCAG CGACCAAAAG TTCCACCAGC
    AAACTCAATC CCAATGAACT AGAGAATGTA GCTACCATTG AAAGGTTATT ACTTCAGTTG
    GATGAGACAG AAAAAGCCTT TAATCAGTTT TGGTCTGAGC ATCACTCAAA GCTTAACCAA
    TGCCTACAAC TACAACACTT TGAGCACAAT TTTTGTGAGG TTAAGCTTGC CCTGGACAAT
    TTGTTGGCAG AGCAAGCGGA GTTTACAGAC ATTGGAGACA GTGTGATTCG TGTGGAGCAA
    ATACTTAAGG AACACAAAAA ACTGGAAGAA AAAGGCCAGG AGCCCTTGGA AAAGGCCCAG
    CTGCTTGCAA TAATTGGGGA CCAGCTCATC CAAAGCCACC ACTATGCAAT GGATTCCATC
    AGACCGAGAT GTGTAGAGCT CAGGCACCTC TGTGATGACT TCATCAATGA AAACAAGAAA
    AAACATGACA TTTTACAAAA GTCTTTGGAG CTGCATAGAC AGCTGGACAA GGTCAGCCAG
    TGGTGTGAAA CAGGAATCTA CCTTTTGGCT TCCCAAGCTG TAGACAAGTG CCAGTCACGA
    GAGGGGGTTG ACGTTGCCTT GAATGACATC GAGGCATTCC TGAGTACGGC GAAGGAGTAC
    CAGTTGCCCA GCCACATGGA ATTTTACAGC CAGTTTGAGT TGATACTCAC CATTGATGTA
    AAGGCCAAAG CCCAGGAAGT ACTGCAGAAG CTGTGCGATG TACAGGAAAT ATTTCACAAG
    AGGCAAATGA GTTTGATGAA ACTGGCAGCC AGACAGACTC GCCCAGTGCA ACCTGTGGCC
    CCTCATCCTG AGTCCTCCCC AAAATGGATG TCACCAAAAA CCAGCCAGCC CTTCACCTTG
    GCTCCTCTTC AGAGAACATC TGAGGAAACT TATACAGGGA CATTAGAAAA GGAAGATGAT
    GAGACTAAAT TTGAAGTCAA GAATGAAGAA ATACTGGAAA GCAGTCATGA TGGGGAGAAC
    CCTGGACAAG AGCAGCTTGA GAGTCCTGAA AATCTTTCCC CTACCAGGCG CATTATACAT
    GACTTGCTTG AGACGGAAGA GATTTATATA AAAGAGATTA AGAGCATAAT TGATGGATAT
    ATCACTCCAA TGGATTTTAT TTGGCTGAAG CATCTGATTC CAGACGTTCT TCAGAATAAC
    AAGGAGTTTC TCTTTGGGAA TATTAGAGAA CTTTATGAAT TTCACAACAG GACTTTTCTG
    AAAGAACTGG AAAAGTGCAC TGAAAACCCC GAAATGCTGG CACGCTGCTT TCTCGAGAGA
    AAAGAAGATC TTCAAATATA TTTTAAATAC CATAAGAATC TGCCCCGAGC TAGAGCAATC
    TGGCAAGAAT GTCAAGACTG CACCTTCTTT GGGGTTTGCC AGCGCCAACT GGATCACAAT
    CTCCCTCTTT TTAAGTATCT CAGAGGACCA AGCCAGAGAC TTATAAAATA TCAGATGCTG
    CTGAAGGGTC TGCTGGATTT TGAGTCTCCT GAGGATTTGG GAATAGACAC AGGTGACCAG
    GAAGGAAGCT TGGCTAAGAA TGGGCCAAAG AGAACCAAGG ATTCAGCTTT CTCAGCCGAA
    CTACAACAGG CTTTGGCCAT GATGGAAGAT TTGATCAAGT CCTGTGAATT GGCCGTGGAC
    CTGGCAGCTG TCACTGGATG TCCGGATGAT ATCAGGAAAC TGGGAAAGCT ACTGAAGCAT
    GGCTCTTTCA ACGTGTGGAC AATTCACAAG GATCGTTATA AAATGAAGGA TTTTATTCGA
    TTTAAGCCCA GACAGAGGCA AATCTATCTA TTTGAAAGGG GAATTGTATT CTGTAAGATA
    CGAATGGAGC CCAGTGACCA GGGCCTATCT CCTCATTACA GCTTTAAGAA ATCTATGAAG
    TTGATGACAC TTTCAATTCG CCAGCTTGGA AGGGGGAGCA ACCGGAAGTT TGAACTTGCC
    AACCGGAATG GACTCGAGAA ATACATCCTG CAGGCAGCTT CGAAAGAAAT CAGAGATTGT
    TGGTTTTCAG AAATAAGTAA ATTACTGATG GAACAACAAA ACAATATTAA AGATATTTAT
    GAAGATGAAG AAAATGGCAA ACAAATCTCC CCCAAATGCT GCTCCAGCAG CGAGTGTATC
    TCCATGGAAA CCTTTGAGGA GCGGAAAGGT GCAGAAGATA TGGAAAAGGA GAGCAGTGCT
    CTGAGTCTAT CCGGACTCTT CCAGTTGGAC GACAGTTACG AAACGTGTTC CTCCAAACCT
    GTCCTGGCCA CAGGAGAAAG CATCCCGGGA AAAAGAGAAG GTGGAGACGA GGACATGAAG
    CCTTCAGGCA TCGCCACATT TCTCCTGGGG TGGGAGTGCG GAGATCCGTT GAGAACCTCC
    TATCGGAGCC TTGGTGGAAG CGGGTCGCTC TTCTCCCAGG ATCCCCCCAA GAATGTTGGC
    GTGGTGTTTG TTACTTTGTT CCCGGATTCT ATCTTTTCAA GTGGGAACTT CTTTCTTAAG
    CACCCGAGGA TTGTCTCCTG TGTGACTCAA GAAAATCACT TCAGAATTCA AGAAAACAAA
    AATAAGCATC TCACTTTATT CATAAAGAAG CCCTTGACTT CGTGGCCAAA AAAAGGGCAG
    AGACTAAGGG ACAGACGATC CGGGACAGGC TCTTCAGCAA AATTGGCCAA CAGAGTGTTC
    TTTGAAATTT TTCAGAAAGT TCTGTTGTGG AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe284496
    Clone ID Related Accession (Same CDS sequence) XM_022414339.1 , XM_022414338.1 , XM_022414340.1
    Accession Version XM_022414340.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3036bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product probable guanine nucleotide exchange factor MCF2L2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAAAGTTC CGATCATTAT GTTAAACTCT GCATCTGACC TTCACGGCTA CATTGACAAA 
    AACCAACTGA CAGAGGATTT AGGGGGAACT TTGGAATACC GCCACAGTCA GTGGATAAAT
    CATCGAACTG CCATTGAAAA TTTTGCCATG ACATTGAAGA CCACTGCCCA GATGCTTCAG
    ATGTTTGGGG CCTGCCTAAC CACAACAGAG CTGCCCACAG GTGTGCTGTC CACGGAAGAC
    CTCCTCATGT CCCACACAAG GCAGCAGGAC AAGCTGCAGG ATGAGCTGAA ATTACTTGGA
    AAGCAAGGGA CCACATTGCT GTCGTGTATT CAAGAACCAG CGACCAAAAG TTCCACCAGC
    AAACTCAATC CCAATGAACT AGAGAATGTA GCTACCATTG AAAGGTTATT ACTTCAGTTG
    GATGAGACAG AAAAAGCCTT TAATCAGTTT TGGTCTGAGC ATCACTCAAA GCTTAACCAA
    TGCCTACAAC TACAACACTT TGAGCACAAT TTTTGTGAGG TTAAGCTTGC CCTGGACAAT
    TTGTTGGCAG AGCAAGCGGA GTTTACAGAC ATTGGAGACA GTGTGATTCG TGTGGAGCAA
    ATACTTAAGG AACACAAAAA ACTGGAAGAA AAAGGCCAGG AGCCCTTGGA AAAGGCCCAG
    CTGCTTGCAA TAATTGGGGA CCAGCTCATC CAAAGCCACC ACTATGCAAT GGATTCCATC
    AGACCGAGAT GTGTAGAGCT CAGGCACCTC TGTGATGACT TCATCAATGA AAACAAGAAA
    AAACATGACA TTTTACAAAA GTCTTTGGAG CTGCATAGAC AGCTGGACAA GGTCAGCCAG
    TGGTGTGAAA CAGGAATCTA CCTTTTGGCT TCCCAAGCTG TAGACAAGTG CCAGTCACGA
    GAGGGGGTTG ACGTTGCCTT GAATGACATC GAGGCATTCC TGAGTACGGC GAAGGAGTAC
    CAGTTGCCCA GCCACATGGA ATTTTACAGC CAGTTTGAGT TGATACTCAC CATTGATGTA
    AAGGCCAAAG CCCAGGAAGT ACTGCAGAAG CTGTGCGATG TACAGGAAAT ATTTCACAAG
    AGGCAAATGA GTTTGATGAA ACTGGCAGCC AGACAGACTC GCCCAGTGCA ACCTGTGGCC
    CCTCATCCTG AGTCCTCCCC AAAATGGATG TCACCAAAAA CCAGCCAGCC CTTCACCTTG
    GCTCCTCTTC AGAGAACATC TGAGGAAACT TATACAGGGA CATTAGAAAA GGAAGATGAT
    GAGACTAAAT TTGAAGTCAA GAATGAAGAA ATACTGGAAA GCAGTCATGA TGGGGAGAAC
    CCTGGACAAG AGCAGCTTGA GAGTCCTGAA AATCTTTCCC CTACCAGGCG CATTATACAT
    GACTTGCTTG AGACGGAAGA GATTTATATA AAAGAGATTA AGAGCATAAT TGATGGATAT
    ATCACTCCAA TGGATTTTAT TTGGCTGAAG CATCTGATTC CAGACGTTCT TCAGAATAAC
    AAGGAGTTTC TCTTTGGGAA TATTAGAGAA CTTTATGAAT TTCACAACAG GACTTTTCTG
    AAAGAACTGG AAAAGTGCAC TGAAAACCCC GAAATGCTGG CACGCTGCTT TCTCGAGAGA
    AAAGAAGATC TTCAAATATA TTTTAAATAC CATAAGAATC TGCCCCGAGC TAGAGCAATC
    TGGCAAGAAT GTCAAGACTG CACCTTCTTT GGGGTTTGCC AGCGCCAACT GGATCACAAT
    CTCCCTCTTT TTAAGTATCT CAGAGGACCA AGCCAGAGAC TTATAAAATA TCAGATGCTG
    CTGAAGGGTC TGCTGGATTT TGAGTCTCCT GAGGATTTGG GAATAGACAC AGGTGACCAG
    GAAGGAAGCT TGGCTAAGAA TGGGCCAAAG AGAACCAAGG ATTCAGCTTT CTCAGCCGAA
    CTACAACAGG CTTTGGCCAT GATGGAAGAT TTGATCAAGT CCTGTGAATT GGCCGTGGAC
    CTGGCAGCTG TCACTGGATG TCCGGATGAT ATCAGGAAAC TGGGAAAGCT ACTGAAGCAT
    GGCTCTTTCA ACGTGTGGAC AATTCACAAG GATCGTTATA AAATGAAGGA TTTTATTCGA
    TTTAAGCCCA GACAGAGGCA AATCTATCTA TTTGAAAGGG GAATTGTATT CTGTAAGATA
    CGAATGGAGC CCAGTGACCA GGGCCTATCT CCTCATTACA GCTTTAAGAA ATCTATGAAG
    TTGATGACAC TTTCAATTCG CCAGCTTGGA AGGGGGAGCA ACCGGAAGTT TGAACTTGCC
    AACCGGAATG GACTCGAGAA ATACATCCTG CAGGCAGCTT CGAAAGAAAT CAGAGATTGT
    TGGTTTTCAG AAATAAGTAA ATTACTGATG GAACAACAAA ACAATATTAA AGATATTTAT
    GAAGATGAAG AAAATGGCAA ACAAATCTCC CCCAAATGCT GCTCCAGCAG CGAGTGTATC
    TCCATGGAAA CCTTTGAGGA GCGGAAAGGT GCAGAAGATA TGGAAAAGGA GAGCAGTGCT
    CTGAGTCTAT CCGGACTCTT CCAGTTGGAC GACAGTTACG AAACGTGTTC CTCCAAACCT
    GTCCTGGCCA CAGGAGAAAG CATCCCGGGA AAAAGAGAAG GTGGAGACGA GGACATGAAG
    CCTTCAGGCA TCGCCACATT TCTCCTGGGG TGGGAGTGCG GAGATCCGTT GAGAACCTCC
    TATCGGAGCC TTGGTGGAAG CGGGTCGCTC TTCTCCCAGG ATCCCCCCAA GAATGTTGGC
    GTGGTGTTTG TTACTTTGTT CCCGGATTCT ATCTTTTCAA GTGGGAACTT CTTTCTTAAG
    CACCCGAGGA TTGTCTCCTG TGTGACTCAA GAAAATCACT TCAGAATTCA AGAAAACAAA
    AATAAGCATC TCACTTTATT CATAAAGAAG CCCTTGACTT CGTGGCCAAA AAAAGGGCAG
    AGACTAAGGG ACAGACGATC CGGGACAGGC TCTTCAGCAA AATTGGCCAA CAGAGTGTTC
    TTTGAAATTT TTCAGAAAGT TCTGTTGTGG AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022270048.1
    CDS353..3388
    Translation

    Target ORF information:

    RefSeq Version XM_022414340.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022414340.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAAAGTTC CGATCATTAT GTTAAACTCT GCATCTGACC TTCACGGCTA CATTGACAAA 
    AACCAACTGA CAGAGGATTT AGGGGGAACT TTGGAATACC GCCACAGTCA GTGGATAAAT
    CATCGAACTG CCATTGAAAA TTTTGCCATG ACATTGAAGA CCACTGCCCA GATGCTTCAG
    ATGTTTGGGG CCTGCCTAAC CACAACAGAG CTGCCCACAG GTGTGCTGTC CACGGAAGAC
    CTCCTCATGT CCCACACAAG GCAGCAGGAC AAGCTGCAGG ATGAGCTGAA ATTACTTGGA
    AAGCAAGGGA CCACATTGCT GTCGTGTATT CAAGAACCAG CGACCAAAAG TTCCACCAGC
    AAACTCAATC CCAATGAACT AGAGAATGTA GCTACCATTG AAAGGTTATT ACTTCAGTTG
    GATGAGACAG AAAAAGCCTT TAATCAGTTT TGGTCTGAGC ATCACTCAAA GCTTAACCAA
    TGCCTACAAC TACAACACTT TGAGCACAAT TTTTGTGAGG TTAAGCTTGC CCTGGACAAT
    TTGTTGGCAG AGCAAGCGGA GTTTACAGAC ATTGGAGACA GTGTGATTCG TGTGGAGCAA
    ATACTTAAGG AACACAAAAA ACTGGAAGAA AAAGGCCAGG AGCCCTTGGA AAAGGCCCAG
    CTGCTTGCAA TAATTGGGGA CCAGCTCATC CAAAGCCACC ACTATGCAAT GGATTCCATC
    AGACCGAGAT GTGTAGAGCT CAGGCACCTC TGTGATGACT TCATCAATGA AAACAAGAAA
    AAACATGACA TTTTACAAAA GTCTTTGGAG CTGCATAGAC AGCTGGACAA GGTCAGCCAG
    TGGTGTGAAA CAGGAATCTA CCTTTTGGCT TCCCAAGCTG TAGACAAGTG CCAGTCACGA
    GAGGGGGTTG ACGTTGCCTT GAATGACATC GAGGCATTCC TGAGTACGGC GAAGGAGTAC
    CAGTTGCCCA GCCACATGGA ATTTTACAGC CAGTTTGAGT TGATACTCAC CATTGATGTA
    AAGGCCAAAG CCCAGGAAGT ACTGCAGAAG CTGTGCGATG TACAGGAAAT ATTTCACAAG
    AGGCAAATGA GTTTGATGAA ACTGGCAGCC AGACAGACTC GCCCAGTGCA ACCTGTGGCC
    CCTCATCCTG AGTCCTCCCC AAAATGGATG TCACCAAAAA CCAGCCAGCC CTTCACCTTG
    GCTCCTCTTC AGAGAACATC TGAGGAAACT TATACAGGGA CATTAGAAAA GGAAGATGAT
    GAGACTAAAT TTGAAGTCAA GAATGAAGAA ATACTGGAAA GCAGTCATGA TGGGGAGAAC
    CCTGGACAAG AGCAGCTTGA GAGTCCTGAA AATCTTTCCC CTACCAGGCG CATTATACAT
    GACTTGCTTG AGACGGAAGA GATTTATATA AAAGAGATTA AGAGCATAAT TGATGGATAT
    ATCACTCCAA TGGATTTTAT TTGGCTGAAG CATCTGATTC CAGACGTTCT TCAGAATAAC
    AAGGAGTTTC TCTTTGGGAA TATTAGAGAA CTTTATGAAT TTCACAACAG GACTTTTCTG
    AAAGAACTGG AAAAGTGCAC TGAAAACCCC GAAATGCTGG CACGCTGCTT TCTCGAGAGA
    AAAGAAGATC TTCAAATATA TTTTAAATAC CATAAGAATC TGCCCCGAGC TAGAGCAATC
    TGGCAAGAAT GTCAAGACTG CACCTTCTTT GGGGTTTGCC AGCGCCAACT GGATCACAAT
    CTCCCTCTTT TTAAGTATCT CAGAGGACCA AGCCAGAGAC TTATAAAATA TCAGATGCTG
    CTGAAGGGTC TGCTGGATTT TGAGTCTCCT GAGGATTTGG GAATAGACAC AGGTGACCAG
    GAAGGAAGCT TGGCTAAGAA TGGGCCAAAG AGAACCAAGG ATTCAGCTTT CTCAGCCGAA
    CTACAACAGG CTTTGGCCAT GATGGAAGAT TTGATCAAGT CCTGTGAATT GGCCGTGGAC
    CTGGCAGCTG TCACTGGATG TCCGGATGAT ATCAGGAAAC TGGGAAAGCT ACTGAAGCAT
    GGCTCTTTCA ACGTGTGGAC AATTCACAAG GATCGTTATA AAATGAAGGA TTTTATTCGA
    TTTAAGCCCA GACAGAGGCA AATCTATCTA TTTGAAAGGG GAATTGTATT CTGTAAGATA
    CGAATGGAGC CCAGTGACCA GGGCCTATCT CCTCATTACA GCTTTAAGAA ATCTATGAAG
    TTGATGACAC TTTCAATTCG CCAGCTTGGA AGGGGGAGCA ACCGGAAGTT TGAACTTGCC
    AACCGGAATG GACTCGAGAA ATACATCCTG CAGGCAGCTT CGAAAGAAAT CAGAGATTGT
    TGGTTTTCAG AAATAAGTAA ATTACTGATG GAACAACAAA ACAATATTAA AGATATTTAT
    GAAGATGAAG AAAATGGCAA ACAAATCTCC CCCAAATGCT GCTCCAGCAG CGAGTGTATC
    TCCATGGAAA CCTTTGAGGA GCGGAAAGGT GCAGAAGATA TGGAAAAGGA GAGCAGTGCT
    CTGAGTCTAT CCGGACTCTT CCAGTTGGAC GACAGTTACG AAACGTGTTC CTCCAAACCT
    GTCCTGGCCA CAGGAGAAAG CATCCCGGGA AAAAGAGAAG GTGGAGACGA GGACATGAAG
    CCTTCAGGCA TCGCCACATT TCTCCTGGGG TGGGAGTGCG GAGATCCGTT GAGAACCTCC
    TATCGGAGCC TTGGTGGAAG CGGGTCGCTC TTCTCCCAGG ATCCCCCCAA GAATGTTGGC
    GTGGTGTTTG TTACTTTGTT CCCGGATTCT ATCTTTTCAA GTGGGAACTT CTTTCTTAAG
    CACCCGAGGA TTGTCTCCTG TGTGACTCAA GAAAATCACT TCAGAATTCA AGAAAACAAA
    AATAAGCATC TCACTTTATT CATAAAGAAG CCCTTGACTT CGTGGCCAAA AAAAGGGCAG
    AGACTAAGGG ACAGACGATC CGGGACAGGC TCTTCAGCAA AATTGGCCAA CAGAGTGTTC
    TTTGAAATTT TTCAGAAAGT TCTGTTGTGG AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe284497
    Clone ID Related Accession (Same CDS sequence) XM_022414341.1
    Accession Version XM_022414341.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3018bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product probable guanine nucleotide exchange factor MCF2L2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTTAAACT CTGCATCTGA CCTTCACGGC TACATTGACA AAAACCAACT GACAGAGGAT 
    TTAGGGGGAA CTTTGGAATA CCGCCACAGT CAGTGGATAA ATCATCGAAC TGCCATTGAA
    AATTTTGCCA TGACATTGAA GACCACTGCC CAGATGCTTC AGATGTTTGG GGCCTGCCTA
    ACCACAACAG AGCTGCCCAC AGGTGTGCTG TCCACGGAAG ACCTCCTCAT GTCCCACACA
    AGGCAGCAGG ACAAGCTGCA GGATGAGCTG AAATTACTTG GAAAGCAAGG GACCACATTG
    CTGTCGTGTA TTCAAGAACC AGCGACCAAA AGTTCCACCA GCAAACTCAA TCCCAATGAA
    CTAGAGAATG TAGCTACCAT TGAAAGGTTA TTACTTCAGT TGGATGAGAC AGAAAAAGCC
    TTTAATCAGT TTTGGTCTGA GCATCACTCA AAGCTTAACC AATGCCTACA ACTACAACAC
    TTTGAGCACA ATTTTTGTGA GGTTAAGCTT GCCCTGGACA ATTTGTTGGC AGAGCAAGCG
    GAGTTTACAG ACATTGGAGA CAGTGTGATT CGTGTGGAGC AAATACTTAA GGAACACAAA
    AAACTGGAAG AAAAAGGCCA GGAGCCCTTG GAAAAGGCCC AGCTGCTTGC AATAATTGGG
    GACCAGCTCA TCCAAAGCCA CCACTATGCA ATGGATTCCA TCAGACCGAG ATGTGTAGAG
    CTCAGGCACC TCTGTGATGA CTTCATCAAT GAAAACAAGA AAAAACATGA CATTTTACAA
    AAGTCTTTGG AGCTGCATAG ACAGCTGGAC AAGGTCAGCC AGTGGTGTGA AACAGGAATC
    TACCTTTTGG CTTCCCAAGC TGTAGACAAG TGCCAGTCAC GAGAGGGGGT TGACGTTGCC
    TTGAATGACA TCGAGGCATT CCTGAGTACG GCGAAGGAGT ACCAGTTGCC CAGCCACATG
    GAATTTTACA GCCAGTTTGA GTTGATACTC ACCATTGATG TAAAGGCCAA AGCCCAGGAA
    GTACTGCAGA AGCTGTGCGA TGTACAGGAA ATATTTCACA AGAGGCAAAT GAGTTTGATG
    AAACTGGCAG CCAGACAGAC TCGCCCAGTG CAACCTGTGG CCCCTCATCC TGAGTCCTCC
    CCAAAATGGA TGTCACCAAA AACCAGCCAG CCCTTCACCT TGGCTCCTCT TCAGAGAACA
    TCTGAGGAAA CTTATACAGG GACATTAGAA AAGGAAGATG ATGAGACTAA ATTTGAAGTC
    AAGAATGAAG AAATACTGGA AAGCAGTCAT GATGGGGAGA ACCCTGGACA AGAGCAGCTT
    GAGAGTCCTG AAAATCTTTC CCCTACCAGG CGCATTATAC ATGACTTGCT TGAGACGGAA
    GAGATTTATA TAAAAGAGAT TAAGAGCATA ATTGATGGAT ATATCACTCC AATGGATTTT
    ATTTGGCTGA AGCATCTGAT TCCAGACGTT CTTCAGAATA ACAAGGAGTT TCTCTTTGGG
    AATATTAGAG AACTTTATGA ATTTCACAAC AGGACTTTTC TGAAAGAACT GGAAAAGTGC
    ACTGAAAACC CCGAAATGCT GGCACGCTGC TTTCTCGAGA GAAAAGAAGA TCTTCAAATA
    TATTTTAAAT ACCATAAGAA TCTGCCCCGA GCTAGAGCAA TCTGGCAAGA ATGTCAAGAC
    TGCACCTTCT TTGGGGTTTG CCAGCGCCAA CTGGATCACA ATCTCCCTCT TTTTAAGTAT
    CTCAGAGGAC CAAGCCAGAG ACTTATAAAA TATCAGATGC TGCTGAAGGG TCTGCTGGAT
    TTTGAGTCTC CTGAGGATTT GGGAATAGAC ACAGGTGACC AGGAAGGAAG CTTGGCTAAG
    AATGGGCCAA AGAGAACCAA GGATTCAGCT TTCTCAGCCG AACTACAACA GGCTTTGGCC
    ATGATGGAAG ATTTGATCAA GTCCTGTGAA TTGGCCGTGG ACCTGGCAGC TGTCACTGGA
    TGTCCGGATG ATATCAGGAA ACTGGGAAAG CTACTGAAGC ATGGCTCTTT CAACGTGTGG
    ACAATTCACA AGGATCGTTA TAAAATGAAG GATTTTATTC GATTTAAGCC CAGACAGAGG
    CAAATCTATC TATTTGAAAG GGGAATTGTA TTCTGTAAGA TACGAATGGA GCCCAGTGAC
    CAGGGCCTAT CTCCTCATTA CAGCTTTAAG AAATCTATGA AGTTGATGAC ACTTTCAATT
    CGCCAGCTTG GAAGGGGGAG CAACCGGAAG TTTGAACTTG CCAACCGGAA TGGACTCGAG
    AAATACATCC TGCAGGCAGC TTCGAAAGAA ATCAGAGATT GTTGGTTTTC AGAAATAAGT
    AAATTACTGA TGGAACAACA AAACAATATT AAAGATATTT ATGAAGATGA AGAAAATGGC
    AAACAAATCT CCCCCAAATG CTGCTCCAGC AGCGAGTGTA TCTCCATGGA AACCTTTGAG
    GAGCGGAAAG GTGCAGAAGA TATGGAAAAG GAGAGCAGTG CTCTGAGTCT ATCCGGACTC
    TTCCAGTTGG ACGACAGTTA CGAAACGTGT TCCTCCAAAC CTGTCCTGGC CACAGGAGAA
    AGCATCCCGG GAAAAAGAGA AGGTGGAGAC GAGGACATGA AGCCTTCAGG CATCGCCACA
    TTTCTCCTGG GGTGGGAGTG CGGAGATCCG TTGAGAACCT CCTATCGGAG CCTTGGTGGA
    AGCGGGTCGC TCTTCTCCCA GGATCCCCCC AAGAATGTTG GCGTGGTGTT TGTTACTTTG
    TTCCCGGATT CTATCTTTTC AAGTGGGAAC TTCTTTCTTA AGCACCCGAG GATTGTCTCC
    TGTGTGACTC AAGAAAATCA CTTCAGAATT CAAGAAAACA AAAATAAGCA TCTCACTTTA
    TTCATAAAGA AGCCCTTGAC TTCGTGGCCA AAAAAAGGGC AGAGACTAAG GGACAGACGA
    TCCGGGACAG GCTCTTCAGC AAAATTGGCC AACAGAGTGT TCTTTGAAAT TTTTCAGAAA
    GTTCTGTTGT GGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022270049.1
    CDS133..3150
    Translation

    Target ORF information:

    RefSeq Version XM_022414341.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022414341.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTTAAACT CTGCATCTGA CCTTCACGGC TACATTGACA AAAACCAACT GACAGAGGAT 
    TTAGGGGGAA CTTTGGAATA CCGCCACAGT CAGTGGATAA ATCATCGAAC TGCCATTGAA
    AATTTTGCCA TGACATTGAA GACCACTGCC CAGATGCTTC AGATGTTTGG GGCCTGCCTA
    ACCACAACAG AGCTGCCCAC AGGTGTGCTG TCCACGGAAG ACCTCCTCAT GTCCCACACA
    AGGCAGCAGG ACAAGCTGCA GGATGAGCTG AAATTACTTG GAAAGCAAGG GACCACATTG
    CTGTCGTGTA TTCAAGAACC AGCGACCAAA AGTTCCACCA GCAAACTCAA TCCCAATGAA
    CTAGAGAATG TAGCTACCAT TGAAAGGTTA TTACTTCAGT TGGATGAGAC AGAAAAAGCC
    TTTAATCAGT TTTGGTCTGA GCATCACTCA AAGCTTAACC AATGCCTACA ACTACAACAC
    TTTGAGCACA ATTTTTGTGA GGTTAAGCTT GCCCTGGACA ATTTGTTGGC AGAGCAAGCG
    GAGTTTACAG ACATTGGAGA CAGTGTGATT CGTGTGGAGC AAATACTTAA GGAACACAAA
    AAACTGGAAG AAAAAGGCCA GGAGCCCTTG GAAAAGGCCC AGCTGCTTGC AATAATTGGG
    GACCAGCTCA TCCAAAGCCA CCACTATGCA ATGGATTCCA TCAGACCGAG ATGTGTAGAG
    CTCAGGCACC TCTGTGATGA CTTCATCAAT GAAAACAAGA AAAAACATGA CATTTTACAA
    AAGTCTTTGG AGCTGCATAG ACAGCTGGAC AAGGTCAGCC AGTGGTGTGA AACAGGAATC
    TACCTTTTGG CTTCCCAAGC TGTAGACAAG TGCCAGTCAC GAGAGGGGGT TGACGTTGCC
    TTGAATGACA TCGAGGCATT CCTGAGTACG GCGAAGGAGT ACCAGTTGCC CAGCCACATG
    GAATTTTACA GCCAGTTTGA GTTGATACTC ACCATTGATG TAAAGGCCAA AGCCCAGGAA
    GTACTGCAGA AGCTGTGCGA TGTACAGGAA ATATTTCACA AGAGGCAAAT GAGTTTGATG
    AAACTGGCAG CCAGACAGAC TCGCCCAGTG CAACCTGTGG CCCCTCATCC TGAGTCCTCC
    CCAAAATGGA TGTCACCAAA AACCAGCCAG CCCTTCACCT TGGCTCCTCT TCAGAGAACA
    TCTGAGGAAA CTTATACAGG GACATTAGAA AAGGAAGATG ATGAGACTAA ATTTGAAGTC
    AAGAATGAAG AAATACTGGA AAGCAGTCAT GATGGGGAGA ACCCTGGACA AGAGCAGCTT
    GAGAGTCCTG AAAATCTTTC CCCTACCAGG CGCATTATAC ATGACTTGCT TGAGACGGAA
    GAGATTTATA TAAAAGAGAT TAAGAGCATA ATTGATGGAT ATATCACTCC AATGGATTTT
    ATTTGGCTGA AGCATCTGAT TCCAGACGTT CTTCAGAATA ACAAGGAGTT TCTCTTTGGG
    AATATTAGAG AACTTTATGA ATTTCACAAC AGGACTTTTC TGAAAGAACT GGAAAAGTGC
    ACTGAAAACC CCGAAATGCT GGCACGCTGC TTTCTCGAGA GAAAAGAAGA TCTTCAAATA
    TATTTTAAAT ACCATAAGAA TCTGCCCCGA GCTAGAGCAA TCTGGCAAGA ATGTCAAGAC
    TGCACCTTCT TTGGGGTTTG CCAGCGCCAA CTGGATCACA ATCTCCCTCT TTTTAAGTAT
    CTCAGAGGAC CAAGCCAGAG ACTTATAAAA TATCAGATGC TGCTGAAGGG TCTGCTGGAT
    TTTGAGTCTC CTGAGGATTT GGGAATAGAC ACAGGTGACC AGGAAGGAAG CTTGGCTAAG
    AATGGGCCAA AGAGAACCAA GGATTCAGCT TTCTCAGCCG AACTACAACA GGCTTTGGCC
    ATGATGGAAG ATTTGATCAA GTCCTGTGAA TTGGCCGTGG ACCTGGCAGC TGTCACTGGA
    TGTCCGGATG ATATCAGGAA ACTGGGAAAG CTACTGAAGC ATGGCTCTTT CAACGTGTGG
    ACAATTCACA AGGATCGTTA TAAAATGAAG GATTTTATTC GATTTAAGCC CAGACAGAGG
    CAAATCTATC TATTTGAAAG GGGAATTGTA TTCTGTAAGA TACGAATGGA GCCCAGTGAC
    CAGGGCCTAT CTCCTCATTA CAGCTTTAAG AAATCTATGA AGTTGATGAC ACTTTCAATT
    CGCCAGCTTG GAAGGGGGAG CAACCGGAAG TTTGAACTTG CCAACCGGAA TGGACTCGAG
    AAATACATCC TGCAGGCAGC TTCGAAAGAA ATCAGAGATT GTTGGTTTTC AGAAATAAGT
    AAATTACTGA TGGAACAACA AAACAATATT AAAGATATTT ATGAAGATGA AGAAAATGGC
    AAACAAATCT CCCCCAAATG CTGCTCCAGC AGCGAGTGTA TCTCCATGGA AACCTTTGAG
    GAGCGGAAAG GTGCAGAAGA TATGGAAAAG GAGAGCAGTG CTCTGAGTCT ATCCGGACTC
    TTCCAGTTGG ACGACAGTTA CGAAACGTGT TCCTCCAAAC CTGTCCTGGC CACAGGAGAA
    AGCATCCCGG GAAAAAGAGA AGGTGGAGAC GAGGACATGA AGCCTTCAGG CATCGCCACA
    TTTCTCCTGG GGTGGGAGTG CGGAGATCCG TTGAGAACCT CCTATCGGAG CCTTGGTGGA
    AGCGGGTCGC TCTTCTCCCA GGATCCCCCC AAGAATGTTG GCGTGGTGTT TGTTACTTTG
    TTCCCGGATT CTATCTTTTC AAGTGGGAAC TTCTTTCTTA AGCACCCGAG GATTGTCTCC
    TGTGTGACTC AAGAAAATCA CTTCAGAATT CAAGAAAACA AAAATAAGCA TCTCACTTTA
    TTCATAAAGA AGCCCTTGAC TTCGTGGCCA AAAAAAGGGC AGAGACTAAG GGACAGACGA
    TCCGGGACAG GCTCTTCAGC AAAATTGGCC AACAGAGTGT TCTTTGAAAT TTTTCAGAAA
    GTTCTGTTGT GGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30436
    Clone ID Related Accession (Same CDS sequence) XM_022414334.1 , XM_005639762.2
    Accession Version XM_022414334.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3510bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product probable guanine nucleotide exchange factor MCF2L2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCTATTTT GGTTAAAGGA AGAGATGGCC CCCCAGGAGC TGAGCCGGCG ACTGGCCACA 
    GTGATCACTC ATGTCGATGA AATTATGCAG CAGGAAATCA GGCCCTCAGT GGCAGTGGAT
    ATAATTGAAC AACTTCACAG ACAGTTTGCC ATTCTCTCAG GAGGTCGAGG GAAAGATGGT
    GCACCCATCA TCACTTTCCC AGAGTTTGTG GGGTTCAAAC ACATCCCAGA GGAAGACTTT
    CTGAATGTCA TGACCTACCT GACTAGCATC CCCAGTGTGG AGGCTGCCAG CATTGGATTC
    ATCATTGTTA TTGACCGACG AAGGGACAAG TGGAGCTCCA TAAAAGCATC ATTGACACGA
    ATAGCTGTAG CATTTCCAGG AAACTTGCAG CTCATATTCA TCCTTCGTCC ATCTCGCTTT
    ATCCAGAGGA CATTCACTGA CATTGGCATT AAATATTATC GAGATGAATT TAAAATGAAA
    GTTCCGATCA TTATGTTAAA CTCTGCATCT GACCTTCACG GCTACATTGA CAAAAACCAA
    CTGACAGAGG ATTTAGGGGG AACTTTGGAA TACCGCCACA GTCAGTGGAT AAATCATCGA
    ACTGCCATTG AAAATTTTGC CATGACATTG AAGACCACTG CCCAGATGCT TCAGATGTTT
    GGGGCCTGCC TAACCACAAC AGAGCTGCCC ACAGGTGTGC TGTCCACGGA AGACCTCCTC
    ATGTCCCACA CAAGGCAGCA GGACAAGCTG CAGGATGAGC TGAAATTACT TGGAAAGCAA
    GGGACCACAT TGCTGTCGTG TATTCAAGAA CCAGCGACCA AAAGTTCCAC CAGCAAACTC
    AATCCCAATG AACTAGAGAA TGTAGCTACC ATTGAAAGGT TATTACTTCA GTTGGATGAG
    ACAGAAAAAG CCTTTAATCA GTTTTGGTCT GAGCATCACT CAAAGCTTAA CCAATGCCTA
    CAACTACAAC ACTTTGAGCA CAATTTTTGT GAGGTTAAGC TTGCCCTGGA CAATTTGTTG
    GCAGAGCAAG CGGAGTTTAC AGACATTGGA GACAGTGTGA TTCGTGTGGA GCAAATACTT
    AAGGAACACA AAAAACTGGA AGAAAAAGGC CAGGAGCCCT TGGAAAAGGC CCAGCTGCTT
    GCAATAATTG GGGACCAGCT CATCCAAAGC CACCACTATG CAATGGATTC CATCAGACCG
    AGATGTGTAG AGCTCAGGCA CCTCTGTGAT GACTTCATCA ATGAAAACAA GAAAAAACAT
    GACATTTTAC AAAAGTCTTT GGAGCTGCAT AGACAGCTGG ACAAGGTCAG CCAGTGGTGT
    GAAACAGGAA TCTACCTTTT GGCTTCCCAA GCTGTAGACA AGTGCCAGTC ACGAGAGGGG
    GTTGACGTTG CCTTGAATGA CATCGAGGCA TTCCTGAGTA CGGCGAAGGA GTACCAGTTG
    CCCAGCCACA TGGAATTTTA CAGCCAGTTT GAGTTGATAC TCACCATTGA TGTAAAGGCC
    AAAGCCCAGG AAGTACTGCA GAAGCTGTGC GATGTACAGG AAATATTTCA CAAGAGGCAA
    ATGAGTTTGA TGAAACTGGC AGCCAGACAG ACTCGCCCAG TGCAACCTGT GGCCCCTCAT
    CCTGAGTCCT CCCCAAAATG GATGTCACCA AAAACCAGCC AGCCCTTCAC CTTGGCTCCT
    CTTCAGAGAA CATCTGAGGA AACTTATACA GGGACATTAG AAAAGGAAGA TGATGAGACT
    AAATTTGAAG TCAAGAATGA AGAAATACTG GAAAGCAGTC ATGATGGGGA GAACCCTGGA
    CAAGAGCAGC TTGAGAGTCC TGAAAATCTT TCCCCTACCA GGCGCATTAT ACATGACTTG
    CTTGAGACGG AAGAGATTTA TATAAAAGAG ATTAAGAGCA TAATTGATGG ATATATCACT
    CCAATGGATT TTATTTGGCT GAAGCATCTG ATTCCAGACG TTCTTCAGAA TAACAAGGAG
    TTTCTCTTTG GGAATATTAG AGAACTTTAT GAATTTCACA ACAGGACTTT TCTGAAAGAA
    CTGGAAAAGT GCACTGAAAA CCCCGAAATG CTGGCACGCT GCTTTCTCGA GAGAAAAGAA
    GATCTTCAAA TATATTTTAA ATACCATAAG AATCTGCCCC GAGCTAGAGC AATCTGGCAA
    GAATGTCAAG ACTGCACCTT CTTTGGGGTT TGCCAGCGCC AACTGGATCA CAATCTCCCT
    CTTTTTAAGT ATCTCAGAGG ACCAAGCCAG AGACTTATAA AATATCAGAT GCTGCTGAAG
    GGTCTGCTGG ATTTTGAGTC TCCTGAGGAT TTGGGAATAG ACACAGGTGA CCAGGAAGGA
    AGCTTGGCTA AGAATGGGCC AAAGAGAACC AAGGATTCAG CTTTCTCAGC CGAACTACAA
    CAGGCTTTGG CCATGATGGA AGATTTGATC AAGTCCTGTG AATTGGCCGT GGACCTGGCA
    GCTGTCACTG GATGTCCGGA TGATATCAGG AAACTGGGAA AGCTACTGAA GCATGGCTCT
    TTCAACGTGT GGACAATTCA CAAGGATCGT TATAAAATGA AGGATTTTAT TCGATTTAAG
    CCCAGACAGA GGCAAATCTA TCTATTTGAA AGGGGAATTG TATTCTGTAA GATACGAATG
    GAGCCCAGTG ACCAGGGCCT ATCTCCTCAT TACAGCTTTA AGAAATCTAT GAAGTTGATG
    ACACTTTCAA TTCGCCAGCT TGGAAGGGGG AGCAACCGGA AGTTTGAACT TGCCAACCGG
    AATGGACTCG AGAAATACAT CCTGCAGGCA GCTTCGAAAG AAATCAGAGA TTGTTGGTTT
    TCAGAAATAA GTAAATTACT GATGGAACAA CAAAACAATA TTAAAGATAT TTATGAAGAT
    GAAGAAAATG GCAAACAAAT CTCCCCCAAA TGCTGCTCCA GCAGCGAGTG TATCTCCATG
    GAAACCTTTG AGGAGCGGAA AGGTGCAGAA GATATGGAAA AGGAGAGCAG TGCTCTGAGT
    CTATCCGGAC TCTTCCAGTT GGACGACAGT TACGAAACGT GTTCCTCCAA ACCTGTCCTG
    GCCACAGGAG AAAGCATCCC GGGAAAAAGA GAAGGTGGAG ACGAGGACAT GAAGCCTTCA
    GGCATCGCCA CATTTCTCCT GGGGTGGGAG TGCGGAGATC CGTTGAGAAC CTCCTATCGG
    AGCCTTGGTG GAAGCGGGTC GCTCTTCTCC CAGGATCCCC CCAAGAATGT TGGCGTGGTG
    TTTGTTACTT TGTTCCCGGA TTCTATCTTT TCAAGTGGGA ACTTCTTTCT TAAGCACCCG
    AGGATTGTCT CCTGTGTGAC TCAAGAAAAT CACTTCAGAA TTCAAGAAAA CAAAAATAAG
    CATCTCACTT TATTCATAAA GAAGCCCTTG ACTTCGTGGC CAAAAAAAGG GCAGAGACTA
    AGGGACAGAC GATCCGGGAC AGGCTCTTCA GCAAAATTGG CCAACAGAGT GTTCTTTGAA
    ATTTTTCAGA AAGTTCTGTT GTGGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022270042.1
    CDS414..3923
    Translation

    Target ORF information:

    RefSeq Version XM_022414334.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022414334.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCTATTTT GGTTAAAGGA AGAGATGGCC CCCCAGGAGC TGAGCCGGCG ACTGGCCACA 
    GTGATCACTC ATGTCGATGA AATTATGCAG CAGGAAATCA GGCCCTCAGT GGCAGTGGAT
    ATAATTGAAC AACTTCACAG ACAGTTTGCC ATTCTCTCAG GAGGTCGAGG GAAAGATGGT
    GCACCCATCA TCACTTTCCC AGAGTTTGTG GGGTTCAAAC ACATCCCAGA GGAAGACTTT
    CTGAATGTCA TGACCTACCT GACTAGCATC CCCAGTGTGG AGGCTGCCAG CATTGGATTC
    ATCATTGTTA TTGACCGACG AAGGGACAAG TGGAGCTCCA TAAAAGCATC ATTGACACGA
    ATAGCTGTAG CATTTCCAGG AAACTTGCAG CTCATATTCA TCCTTCGTCC ATCTCGCTTT
    ATCCAGAGGA CATTCACTGA CATTGGCATT AAATATTATC GAGATGAATT TAAAATGAAA
    GTTCCGATCA TTATGTTAAA CTCTGCATCT GACCTTCACG GCTACATTGA CAAAAACCAA
    CTGACAGAGG ATTTAGGGGG AACTTTGGAA TACCGCCACA GTCAGTGGAT AAATCATCGA
    ACTGCCATTG AAAATTTTGC CATGACATTG AAGACCACTG CCCAGATGCT TCAGATGTTT
    GGGGCCTGCC TAACCACAAC AGAGCTGCCC ACAGGTGTGC TGTCCACGGA AGACCTCCTC
    ATGTCCCACA CAAGGCAGCA GGACAAGCTG CAGGATGAGC TGAAATTACT TGGAAAGCAA
    GGGACCACAT TGCTGTCGTG TATTCAAGAA CCAGCGACCA AAAGTTCCAC CAGCAAACTC
    AATCCCAATG AACTAGAGAA TGTAGCTACC ATTGAAAGGT TATTACTTCA GTTGGATGAG
    ACAGAAAAAG CCTTTAATCA GTTTTGGTCT GAGCATCACT CAAAGCTTAA CCAATGCCTA
    CAACTACAAC ACTTTGAGCA CAATTTTTGT GAGGTTAAGC TTGCCCTGGA CAATTTGTTG
    GCAGAGCAAG CGGAGTTTAC AGACATTGGA GACAGTGTGA TTCGTGTGGA GCAAATACTT
    AAGGAACACA AAAAACTGGA AGAAAAAGGC CAGGAGCCCT TGGAAAAGGC CCAGCTGCTT
    GCAATAATTG GGGACCAGCT CATCCAAAGC CACCACTATG CAATGGATTC CATCAGACCG
    AGATGTGTAG AGCTCAGGCA CCTCTGTGAT GACTTCATCA ATGAAAACAA GAAAAAACAT
    GACATTTTAC AAAAGTCTTT GGAGCTGCAT AGACAGCTGG ACAAGGTCAG CCAGTGGTGT
    GAAACAGGAA TCTACCTTTT GGCTTCCCAA GCTGTAGACA AGTGCCAGTC ACGAGAGGGG
    GTTGACGTTG CCTTGAATGA CATCGAGGCA TTCCTGAGTA CGGCGAAGGA GTACCAGTTG
    CCCAGCCACA TGGAATTTTA CAGCCAGTTT GAGTTGATAC TCACCATTGA TGTAAAGGCC
    AAAGCCCAGG AAGTACTGCA GAAGCTGTGC GATGTACAGG AAATATTTCA CAAGAGGCAA
    ATGAGTTTGA TGAAACTGGC AGCCAGACAG ACTCGCCCAG TGCAACCTGT GGCCCCTCAT
    CCTGAGTCCT CCCCAAAATG GATGTCACCA AAAACCAGCC AGCCCTTCAC CTTGGCTCCT
    CTTCAGAGAA CATCTGAGGA AACTTATACA GGGACATTAG AAAAGGAAGA TGATGAGACT
    AAATTTGAAG TCAAGAATGA AGAAATACTG GAAAGCAGTC ATGATGGGGA GAACCCTGGA
    CAAGAGCAGC TTGAGAGTCC TGAAAATCTT TCCCCTACCA GGCGCATTAT ACATGACTTG
    CTTGAGACGG AAGAGATTTA TATAAAAGAG ATTAAGAGCA TAATTGATGG ATATATCACT
    CCAATGGATT TTATTTGGCT GAAGCATCTG ATTCCAGACG TTCTTCAGAA TAACAAGGAG
    TTTCTCTTTG GGAATATTAG AGAACTTTAT GAATTTCACA ACAGGACTTT TCTGAAAGAA
    CTGGAAAAGT GCACTGAAAA CCCCGAAATG CTGGCACGCT GCTTTCTCGA GAGAAAAGAA
    GATCTTCAAA TATATTTTAA ATACCATAAG AATCTGCCCC GAGCTAGAGC AATCTGGCAA
    GAATGTCAAG ACTGCACCTT CTTTGGGGTT TGCCAGCGCC AACTGGATCA CAATCTCCCT
    CTTTTTAAGT ATCTCAGAGG ACCAAGCCAG AGACTTATAA AATATCAGAT GCTGCTGAAG
    GGTCTGCTGG ATTTTGAGTC TCCTGAGGAT TTGGGAATAG ACACAGGTGA CCAGGAAGGA
    AGCTTGGCTA AGAATGGGCC AAAGAGAACC AAGGATTCAG CTTTCTCAGC CGAACTACAA
    CAGGCTTTGG CCATGATGGA AGATTTGATC AAGTCCTGTG AATTGGCCGT GGACCTGGCA
    GCTGTCACTG GATGTCCGGA TGATATCAGG AAACTGGGAA AGCTACTGAA GCATGGCTCT
    TTCAACGTGT GGACAATTCA CAAGGATCGT TATAAAATGA AGGATTTTAT TCGATTTAAG
    CCCAGACAGA GGCAAATCTA TCTATTTGAA AGGGGAATTG TATTCTGTAA GATACGAATG
    GAGCCCAGTG ACCAGGGCCT ATCTCCTCAT TACAGCTTTA AGAAATCTAT GAAGTTGATG
    ACACTTTCAA TTCGCCAGCT TGGAAGGGGG AGCAACCGGA AGTTTGAACT TGCCAACCGG
    AATGGACTCG AGAAATACAT CCTGCAGGCA GCTTCGAAAG AAATCAGAGA TTGTTGGTTT
    TCAGAAATAA GTAAATTACT GATGGAACAA CAAAACAATA TTAAAGATAT TTATGAAGAT
    GAAGAAAATG GCAAACAAAT CTCCCCCAAA TGCTGCTCCA GCAGCGAGTG TATCTCCATG
    GAAACCTTTG AGGAGCGGAA AGGTGCAGAA GATATGGAAA AGGAGAGCAG TGCTCTGAGT
    CTATCCGGAC TCTTCCAGTT GGACGACAGT TACGAAACGT GTTCCTCCAA ACCTGTCCTG
    GCCACAGGAG AAAGCATCCC GGGAAAAAGA GAAGGTGGAG ACGAGGACAT GAAGCCTTCA
    GGCATCGCCA CATTTCTCCT GGGGTGGGAG TGCGGAGATC CGTTGAGAAC CTCCTATCGG
    AGCCTTGGTG GAAGCGGGTC GCTCTTCTCC CAGGATCCCC CCAAGAATGT TGGCGTGGTG
    TTTGTTACTT TGTTCCCGGA TTCTATCTTT TCAAGTGGGA ACTTCTTTCT TAAGCACCCG
    AGGATTGTCT CCTGTGTGAC TCAAGAAAAT CACTTCAGAA TTCAAGAAAA CAAAAATAAG
    CATCTCACTT TATTCATAAA GAAGCCCTTG ACTTCGTGGC CAAAAAAAGG GCAGAGACTA
    AGGGACAGAC GATCCGGGAC AGGCTCTTCA GCAAAATTGG CCAACAGAGT GTTCTTTGAA
    ATTTTTCAGA AAGTTCTGTT GTGGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30436
    Clone ID Related Accession (Same CDS sequence) XM_022414334.1 , XM_005639762.2
    Accession Version XM_005639762.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3510bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product probable guanine nucleotide exchange factor MCF2L2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726120.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005639762.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCTATTTT GGTTAAAGGA AGAGATGGCC CCCCAGGAGC TGAGCCGGCG ACTGGCCACA 
    GTGATCACTC ATGTCGATGA AATTATGCAG CAGGAAATCA GGCCCTCAGT GGCAGTGGAT
    ATAATTGAAC AACTTCACAG ACAGTTTGCC ATTCTCTCAG GAGGTCGAGG GAAAGATGGT
    GCACCCATCA TCACTTTCCC AGAGTTTGTG GGGTTCAAAC ACATCCCAGA GGAAGACTTT
    CTGAATGTCA TGACCTACCT GACTAGCATC CCCAGTGTGG AGGCTGCCAG CATTGGATTC
    ATCATTGTTA TTGACCGACG AAGGGACAAG TGGAGCTCCA TAAAAGCATC ATTGACACGA
    ATAGCTGTAG CATTTCCAGG AAACTTGCAG CTCATATTCA TCCTTCGTCC ATCTCGCTTT
    ATCCAGAGGA CATTCACTGA CATTGGCATT AAATATTATC GAGATGAATT TAAAATGAAA
    GTTCCGATCA TTATGTTAAA CTCTGCATCT GACCTTCACG GCTACATTGA CAAAAACCAA
    CTGACAGAGG ATTTAGGGGG AACTTTGGAA TACCGCCACA GTCAGTGGAT AAATCATCGA
    ACTGCCATTG AAAATTTTGC CATGACATTG AAGACCACTG CCCAGATGCT TCAGATGTTT
    GGGGCCTGCC TAACCACAAC AGAGCTGCCC ACAGGTGTGC TGTCCACGGA AGACCTCCTC
    ATGTCCCACA CAAGGCAGCA GGACAAGCTG CAGGATGAGC TGAAATTACT TGGAAAGCAA
    GGGACCACAT TGCTGTCGTG TATTCAAGAA CCAGCGACCA AAAGTTCCAC CAGCAAACTC
    AATCCCAATG AACTAGAGAA TGTAGCTACC ATTGAAAGGT TATTACTTCA GTTGGATGAG
    ACAGAAAAAG CCTTTAATCA GTTTTGGTCT GAGCATCACT CAAAGCTTAA CCAATGCCTA
    CAACTACAAC ACTTTGAGCA CAATTTTTGT GAGGTTAAGC TTGCCCTGGA CAATTTGTTG
    GCAGAGCAAG CGGAGTTTAC AGACATTGGA GACAGTGTGA TTCGTGTGGA GCAAATACTT
    AAGGAACACA AAAAACTGGA AGAAAAAGGC CAGGAGCCCT TGGAAAAGGC CCAGCTGCTT
    GCAATAATTG GGGACCAGCT CATCCAAAGC CACCACTATG CAATGGATTC CATCAGACCG
    AGATGTGTAG AGCTCAGGCA CCTCTGTGAT GACTTCATCA ATGAAAACAA GAAAAAACAT
    GACATTTTAC AAAAGTCTTT GGAGCTGCAT AGACAGCTGG ACAAGGTCAG CCAGTGGTGT
    GAAACAGGAA TCTACCTTTT GGCTTCCCAA GCTGTAGACA AGTGCCAGTC ACGAGAGGGG
    GTTGACGTTG CCTTGAATGA CATCGAGGCA TTCCTGAGTA CGGCGAAGGA GTACCAGTTG
    CCCAGCCACA TGGAATTTTA CAGCCAGTTT GAGTTGATAC TCACCATTGA TGTAAAGGCC
    AAAGCCCAGG AAGTACTGCA GAAGCTGTGC GATGTACAGG AAATATTTCA CAAGAGGCAA
    ATGAGTTTGA TGAAACTGGC AGCCAGACAG ACTCGCCCAG TGCAACCTGT GGCCCCTCAT
    CCTGAGTCCT CCCCAAAATG GATGTCACCA AAAACCAGCC AGCCCTTCAC CTTGGCTCCT
    CTTCAGAGAA CATCTGAGGA AACTTATACA GGGACATTAG AAAAGGAAGA TGATGAGACT
    AAATTTGAAG TCAAGAATGA AGAAATACTG GAAAGCAGTC ATGATGGGGA GAACCCTGGA
    CAAGAGCAGC TTGAGAGTCC TGAAAATCTT TCCCCTACCA GGCGCATTAT ACATGACTTG
    CTTGAGACGG AAGAGATTTA TATAAAAGAG ATTAAGAGCA TAATTGATGG ATATATCACT
    CCAATGGATT TTATTTGGCT GAAGCATCTG ATTCCAGACG TTCTTCAGAA TAACAAGGAG
    TTTCTCTTTG GGAATATTAG AGAACTTTAT GAATTTCACA ACAGGACTTT TCTGAAAGAA
    CTGGAAAAGT GCACTGAAAA CCCCGAAATG CTGGCACGCT GCTTTCTCGA GAGAAAAGAA
    GATCTTCAAA TATATTTTAA ATACCATAAG AATCTGCCCC GAGCTAGAGC AATCTGGCAA
    GAATGTCAAG ACTGCACCTT CTTTGGGGTT TGCCAGCGCC AACTGGATCA CAATCTCCCT
    CTTTTTAAGT ATCTCAGAGG ACCAAGCCAG AGACTTATAA AATATCAGAT GCTGCTGAAG
    GGTCTGCTGG ATTTTGAGTC TCCTGAGGAT TTGGGAATAG ACACAGGTGA CCAGGAAGGA
    AGCTTGGCTA AGAATGGGCC AAAGAGAACC AAGGATTCAG CTTTCTCAGC CGAACTACAA
    CAGGCTTTGG CCATGATGGA AGATTTGATC AAGTCCTGTG AATTGGCCGT GGACCTGGCA
    GCTGTCACTG GATGTCCGGA TGATATCAGG AAACTGGGAA AGCTACTGAA GCATGGCTCT
    TTCAACGTGT GGACAATTCA CAAGGATCGT TATAAAATGA AGGATTTTAT TCGATTTAAG
    CCCAGACAGA GGCAAATCTA TCTATTTGAA AGGGGAATTG TATTCTGTAA GATACGAATG
    GAGCCCAGTG ACCAGGGCCT ATCTCCTCAT TACAGCTTTA AGAAATCTAT GAAGTTGATG
    ACACTTTCAA TTCGCCAGCT TGGAAGGGGG AGCAACCGGA AGTTTGAACT TGCCAACCGG
    AATGGACTCG AGAAATACAT CCTGCAGGCA GCTTCGAAAG AAATCAGAGA TTGTTGGTTT
    TCAGAAATAA GTAAATTACT GATGGAACAA CAAAACAATA TTAAAGATAT TTATGAAGAT
    GAAGAAAATG GCAAACAAAT CTCCCCCAAA TGCTGCTCCA GCAGCGAGTG TATCTCCATG
    GAAACCTTTG AGGAGCGGAA AGGTGCAGAA GATATGGAAA AGGAGAGCAG TGCTCTGAGT
    CTATCCGGAC TCTTCCAGTT GGACGACAGT TACGAAACGT GTTCCTCCAA ACCTGTCCTG
    GCCACAGGAG AAAGCATCCC GGGAAAAAGA GAAGGTGGAG ACGAGGACAT GAAGCCTTCA
    GGCATCGCCA CATTTCTCCT GGGGTGGGAG TGCGGAGATC CGTTGAGAAC CTCCTATCGG
    AGCCTTGGTG GAAGCGGGTC GCTCTTCTCC CAGGATCCCC CCAAGAATGT TGGCGTGGTG
    TTTGTTACTT TGTTCCCGGA TTCTATCTTT TCAAGTGGGA ACTTCTTTCT TAAGCACCCG
    AGGATTGTCT CCTGTGTGAC TCAAGAAAAT CACTTCAGAA TTCAAGAAAA CAAAAATAAG
    CATCTCACTT TATTCATAAA GAAGCCCTTG ACTTCGTGGC CAAAAAAAGG GCAGAGACTA
    AGGGACAGAC GATCCGGGAC AGGCTCTTCA GCAAAATTGG CCAACAGAGT GTTCTTTGAA
    ATTTTTCAGA AAGTTCTGTT GTGGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005639819.1
    CDS437..3946
    Translation

    Target ORF information:

    RefSeq Version XM_005639762.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005639762.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCTATTTT GGTTAAAGGA AGAGATGGCC CCCCAGGAGC TGAGCCGGCG ACTGGCCACA 
    GTGATCACTC ATGTCGATGA AATTATGCAG CAGGAAATCA GGCCCTCAGT GGCAGTGGAT
    ATAATTGAAC AACTTCACAG ACAGTTTGCC ATTCTCTCAG GAGGTCGAGG GAAAGATGGT
    GCACCCATCA TCACTTTCCC AGAGTTTGTG GGGTTCAAAC ACATCCCAGA GGAAGACTTT
    CTGAATGTCA TGACCTACCT GACTAGCATC CCCAGTGTGG AGGCTGCCAG CATTGGATTC
    ATCATTGTTA TTGACCGACG AAGGGACAAG TGGAGCTCCA TAAAAGCATC ATTGACACGA
    ATAGCTGTAG CATTTCCAGG AAACTTGCAG CTCATATTCA TCCTTCGTCC ATCTCGCTTT
    ATCCAGAGGA CATTCACTGA CATTGGCATT AAATATTATC GAGATGAATT TAAAATGAAA
    GTTCCGATCA TTATGTTAAA CTCTGCATCT GACCTTCACG GCTACATTGA CAAAAACCAA
    CTGACAGAGG ATTTAGGGGG AACTTTGGAA TACCGCCACA GTCAGTGGAT AAATCATCGA
    ACTGCCATTG AAAATTTTGC CATGACATTG AAGACCACTG CCCAGATGCT TCAGATGTTT
    GGGGCCTGCC TAACCACAAC AGAGCTGCCC ACAGGTGTGC TGTCCACGGA AGACCTCCTC
    ATGTCCCACA CAAGGCAGCA GGACAAGCTG CAGGATGAGC TGAAATTACT TGGAAAGCAA
    GGGACCACAT TGCTGTCGTG TATTCAAGAA CCAGCGACCA AAAGTTCCAC CAGCAAACTC
    AATCCCAATG AACTAGAGAA TGTAGCTACC ATTGAAAGGT TATTACTTCA GTTGGATGAG
    ACAGAAAAAG CCTTTAATCA GTTTTGGTCT GAGCATCACT CAAAGCTTAA CCAATGCCTA
    CAACTACAAC ACTTTGAGCA CAATTTTTGT GAGGTTAAGC TTGCCCTGGA CAATTTGTTG
    GCAGAGCAAG CGGAGTTTAC AGACATTGGA GACAGTGTGA TTCGTGTGGA GCAAATACTT
    AAGGAACACA AAAAACTGGA AGAAAAAGGC CAGGAGCCCT TGGAAAAGGC CCAGCTGCTT
    GCAATAATTG GGGACCAGCT CATCCAAAGC CACCACTATG CAATGGATTC CATCAGACCG
    AGATGTGTAG AGCTCAGGCA CCTCTGTGAT GACTTCATCA ATGAAAACAA GAAAAAACAT
    GACATTTTAC AAAAGTCTTT GGAGCTGCAT AGACAGCTGG ACAAGGTCAG CCAGTGGTGT
    GAAACAGGAA TCTACCTTTT GGCTTCCCAA GCTGTAGACA AGTGCCAGTC ACGAGAGGGG
    GTTGACGTTG CCTTGAATGA CATCGAGGCA TTCCTGAGTA CGGCGAAGGA GTACCAGTTG
    CCCAGCCACA TGGAATTTTA CAGCCAGTTT GAGTTGATAC TCACCATTGA TGTAAAGGCC
    AAAGCCCAGG AAGTACTGCA GAAGCTGTGC GATGTACAGG AAATATTTCA CAAGAGGCAA
    ATGAGTTTGA TGAAACTGGC AGCCAGACAG ACTCGCCCAG TGCAACCTGT GGCCCCTCAT
    CCTGAGTCCT CCCCAAAATG GATGTCACCA AAAACCAGCC AGCCCTTCAC CTTGGCTCCT
    CTTCAGAGAA CATCTGAGGA AACTTATACA GGGACATTAG AAAAGGAAGA TGATGAGACT
    AAATTTGAAG TCAAGAATGA AGAAATACTG GAAAGCAGTC ATGATGGGGA GAACCCTGGA
    CAAGAGCAGC TTGAGAGTCC TGAAAATCTT TCCCCTACCA GGCGCATTAT ACATGACTTG
    CTTGAGACGG AAGAGATTTA TATAAAAGAG ATTAAGAGCA TAATTGATGG ATATATCACT
    CCAATGGATT TTATTTGGCT GAAGCATCTG ATTCCAGACG TTCTTCAGAA TAACAAGGAG
    TTTCTCTTTG GGAATATTAG AGAACTTTAT GAATTTCACA ACAGGACTTT TCTGAAAGAA
    CTGGAAAAGT GCACTGAAAA CCCCGAAATG CTGGCACGCT GCTTTCTCGA GAGAAAAGAA
    GATCTTCAAA TATATTTTAA ATACCATAAG AATCTGCCCC GAGCTAGAGC AATCTGGCAA
    GAATGTCAAG ACTGCACCTT CTTTGGGGTT TGCCAGCGCC AACTGGATCA CAATCTCCCT
    CTTTTTAAGT ATCTCAGAGG ACCAAGCCAG AGACTTATAA AATATCAGAT GCTGCTGAAG
    GGTCTGCTGG ATTTTGAGTC TCCTGAGGAT TTGGGAATAG ACACAGGTGA CCAGGAAGGA
    AGCTTGGCTA AGAATGGGCC AAAGAGAACC AAGGATTCAG CTTTCTCAGC CGAACTACAA
    CAGGCTTTGG CCATGATGGA AGATTTGATC AAGTCCTGTG AATTGGCCGT GGACCTGGCA
    GCTGTCACTG GATGTCCGGA TGATATCAGG AAACTGGGAA AGCTACTGAA GCATGGCTCT
    TTCAACGTGT GGACAATTCA CAAGGATCGT TATAAAATGA AGGATTTTAT TCGATTTAAG
    CCCAGACAGA GGCAAATCTA TCTATTTGAA AGGGGAATTG TATTCTGTAA GATACGAATG
    GAGCCCAGTG ACCAGGGCCT ATCTCCTCAT TACAGCTTTA AGAAATCTAT GAAGTTGATG
    ACACTTTCAA TTCGCCAGCT TGGAAGGGGG AGCAACCGGA AGTTTGAACT TGCCAACCGG
    AATGGACTCG AGAAATACAT CCTGCAGGCA GCTTCGAAAG AAATCAGAGA TTGTTGGTTT
    TCAGAAATAA GTAAATTACT GATGGAACAA CAAAACAATA TTAAAGATAT TTATGAAGAT
    GAAGAAAATG GCAAACAAAT CTCCCCCAAA TGCTGCTCCA GCAGCGAGTG TATCTCCATG
    GAAACCTTTG AGGAGCGGAA AGGTGCAGAA GATATGGAAA AGGAGAGCAG TGCTCTGAGT
    CTATCCGGAC TCTTCCAGTT GGACGACAGT TACGAAACGT GTTCCTCCAA ACCTGTCCTG
    GCCACAGGAG AAAGCATCCC GGGAAAAAGA GAAGGTGGAG ACGAGGACAT GAAGCCTTCA
    GGCATCGCCA CATTTCTCCT GGGGTGGGAG TGCGGAGATC CGTTGAGAAC CTCCTATCGG
    AGCCTTGGTG GAAGCGGGTC GCTCTTCTCC CAGGATCCCC CCAAGAATGT TGGCGTGGTG
    TTTGTTACTT TGTTCCCGGA TTCTATCTTT TCAAGTGGGA ACTTCTTTCT TAAGCACCCG
    AGGATTGTCT CCTGTGTGAC TCAAGAAAAT CACTTCAGAA TTCAAGAAAA CAAAAATAAG
    CATCTCACTT TATTCATAAA GAAGCCCTTG ACTTCGTGGC CAAAAAAAGG GCAGAGACTA
    AGGGACAGAC GATCCGGGAC AGGCTCTTCA GCAAAATTGG CCAACAGAGT GTTCTTTGAA
    ATTTTTCAGA AAGTTCTGTT GTGGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.