THSD7A cDNA ORF clone, Canis lupus familiaris(dog)

The following THSD7A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the THSD7A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe16746 XM_005628560.3
Latest version!
Canis lupus familiaris thrombospondin type 1 domain containing 7A (THSD7A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe16747 XM_003431865.5
Latest version!
Canis lupus familiaris thrombospondin type 1 domain containing 7A (THSD7A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe16748 XM_005628561.3
Latest version!
Canis lupus familiaris thrombospondin type 1 domain containing 7A (THSD7A), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCe293241 XM_022427411.1
Latest version!
Canis lupus familiaris thrombospondin type 1 domain containing 7A (THSD7A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe293242 XM_022427412.1
Latest version!
Canis lupus familiaris thrombospondin type 1 domain containing 7A (THSD7A), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe293243 XM_022427413.1
Latest version!
Canis lupus familiaris thrombospondin type 1 domain containing 7A (THSD7A), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
View Old Accession Versions >>
OCe16748 XM_005628561.2 Canis lupus familiaris thrombospondin, type I, domain containing 7A (THSD7A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCe16746 XM_005628560.2 Canis lupus familiaris thrombospondin, type I, domain containing 7A (THSD7A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe16747 XM_003431865.4 Canis lupus familiaris thrombospondin, type I, domain containing 7A (THSD7A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe16746
    Clone ID Related Accession (Same CDS sequence) XM_005628560.3 , XM_005628560.2
    Accession Version XM_005628560.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5037bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product thrombospondin type-1 domain-containing protein 7A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006596.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005628560.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGGGCTGC AAGCGAGGCG CTCGGCGTCC CAGAGCCCCG GCGCCCCGGG CCCGCGCCGG 
    GGTGTCCTGC AGCTGCTGCC GCTGCTCCTG CTGCTGCTGC TGCTGCTGCT GCTGCGCCCC
    GGAGCCTGCG GGGCTGCGGC TCCCGGCGGG GCTGAGGCAC CCACCCTCTA CCTCTGGAAG
    ACTGGTCCAT GGGGCCGGTG TATGGGAGAT GAATGTGGTC CAGGAGGCAT TCAGACCCGG
    GCCGTGTGGT GTGCTCATGT GGAAGGATGG ACCACATTAC ATACAAACTG TAAGCAGGCT
    GAGAGACCCA GCAACCAGCA AAACTGTTTC AAGGTGTGTG ACTGGCACAA GGAGTTGTAT
    GATTGGAGGC TGGGACCTTG GAACCAGTGT CAGCCTGTGA TATCCAAGAG CCTGGAGAAA
    CCCCCTGAGT GCATTAAGGG GGAGGAAGGC ATCCAGGTGA GAGAAATAAC GTGCATCCAG
    AAAGAGAAGG ACATTCCTGC AGAGGATATC ATTTGTGAGT ACTTTGAGCC CAAACCTCTC
    CTGGAACAGG CCTGCCTCAT CCCCTGCCAG CAAGATTGCA TCGTGTCTGA GTTTTCCGCG
    TGGTCAGAAT GTTCCAAGAC TTGTGGCAGT GGGCTCCAGC ACCGGACTCG GCATGTGGTG
    GCGCCTCCCC AGTTTGGAGG TTCTGGCTGC CCAAACTTGA CAGAGTTTCA GGTGTGCCAG
    TCCAGCCCCT GTGAAGATGA AGAGAGCATG TACAGCTTGC ACGTGGGTCC CTGGAGCTCA
    TGCTCGATGT CCCACTCAAG ACAAGTAAGA CAAGCAAGGA GACGTGGGAA GAATAAAGAA
    CGGGAGAAGG ACCGAGGAAA AGGAGTGAAG GATCCCGAAG CCCGTGAGCT TATTAAGAAA
    AAGAGAAACA GAAACAGACA GAATCGACAA GAGAACAAAT ATTGGGACAT CCAGATTGGG
    TATCAGACCA GAGAGGTTAT GTGTACTAAC AAGACGGGAA AAGCTGTTGA TTTGAGCTTT
    TGCCAGCAAG AGAAGCTGCC AATGACCTTC CAGTCCTGTG TGATCACCAA AGAGTGCCAG
    GTGTCAGAGT GGTCAGAATG GAGTCCCTGC TCAAAAACAT GCCATGACAT GACATCCCCC
    AGAGGCACTC GCGTCAGGAC GCGGATCATT AGGCAGTTTC CTATTGGCAG CGAGAAAGAG
    TGTCCAGAAC TTGAAGAAAA AGAACCCTGT GTGTCTCAAG GAGATGCAGT TGCCCCCTGT
    GCCACGTATG GCTGGAGAAC TACAGAGTGG GCGGAGTGCC GTGTGGACCC CTTGCTCAGT
    CAGCAGGACA AGAGGCGGGG CAACCAGACA GCCCTCTGTG GAGGAGGAGT CCAGACACGA
    GAGGTGTACT GCGTGCAGGC CAATGAAAAC CTCCTCTCAC ACTTAAACAC CCACAAGGAC
    AAAGAAGAAA GTAAATATCA GAGTACGTCG AAGTCTAGTG CTGCACCTTT CCTATCCATC
    ACTTTTGAAA TAACAGCCTC AAAACCAGTG GACTTGAAAT CATGCACTGG CCCTGTCCCT
    AATACTACAC AGCTGTGCCA CATTCCCTGT CCTATTGAAT GTGAGGTTTC ACCTTGGTCA
    GCTTGGGGAC CTTGTACTTA TGAAAACTGT AATGACCAGC AAGGCAAAAA AGGTTTCAAA
    CTGAGGAAGC GACGCATTAC CAATGAGCCC ACTGGAGGCT CTGGGGGCAC TGGGAACTGC
    CCTCACTTAC TGGAAGCCAT TCCCTGTGAA GAGCCATCCT GCTATGAATG GAAAGCAGTG
    AGGCTTGGAG ACTGTGAACC AGACAATGGA AAAGAGTGTG GCCCAGGCAC TCAAGTTCAG
    GAGGTTGTAT GCATCAACAG TGATGGAGAA GAAGTTGACA GACAGCTGTG CAGAGATGCC
    ATCTTCCCCA TCCCCGTTGC CTGTTATGCC CCATGCCCAA AGGACTGTGT GCTCAGCACG
    TGGTCTGCAT GGTCCTCCTG CTCACACACC TGCTCGGGGA AAACCACAGA AGGGAAACAG
    ATACGAGCAC GGTCCATTCT GGCCTATGCC GGTGACGAAG GTGGAGCTCA CTGTCCAAAT
    AGCAGTGCTT TGCAAGAGGT GCGCAGTTGT AACGAGCATC CCTGCACTGT GTACCACTGG
    CAGACTGGTC CCTGGGGCCA GTGCATAGAG GACACTTCAG TATCATCCTT CAACACGACG
    ACTACTTGGA ATGGAGAGGC GTCTTGCTCT GTTGGCATGC AGACAAGAAA AGTCATTTGT
    GTGCGGGTCA ATGTGGGCCA AGTAGGACCC AAAAAGTGTC CTGAAAGCCT TAGACCTGAA
    ACAGTGAGGC CCTGTCTGCT TCCTTGTAGA AAGGACTGTA TTGTGACACC ATATAGTGAC
    TGGACATCAT GCCCCAACTC ATGTAAAGAA GGGGACTCCG GAGTCAGAAA GCAGTCTCGG
    CACCGGGTCA TCATTCAGCA GCCGGCCAAC GGGGGCAGGG ACTGCACGGA CCCGCTCTAC
    GAAGAGAAAG TCTGTGAGGC CCCTCAAGTG TGCCAAAGCT ACAGGTGGAA GACCCACAAA
    TGGCGCAGGT GCCAGTTGGT CCCTTGGAGC GTGCGACAGG ACAGCCCTGG AGCACAGGAA
    GGCTGCGGAC CCGGACGCCA GGCAAGAGCT ATTACTTGTC GAAAGCAAGA TGGAGGACAG
    GCTGGCATCC ATGAATGCCT CTGGTATGCA GGCCCTGTGC CAGCCCTCAC CCAGGCCTGC
    CAGATCCCAT GCCAAGATGA CTGTCAGTTT ACCAGCTGGT CCAAGTTTTC TTCCTGCAAC
    GGAGACTGTG GTGCAGTTAG GACCAGAAAA CGCACTATTG TTGGAAAAAG TAAAAAGAAG
    GAAAAATGTA AAAATTCTCA TTTGTACCCC CTGATTGAGA CTCAGTACTG TCCTTGTGAC
    AAGTATAATG CACAGCCTGT GGGGAACTGG TCAGACTGCA TTTTACCAGA GGGGAAAGTG
    GAAGTGTTGC TTGGAGTGAA AACACAAGGA GATACCAAGG AATGTGGTCA AGGATATCGT
    TACCAAGCAA TGGCATGCTA CGACCAAAAT GGCAGGCTCG TGGAAACATC CAGATGTAAC
    AGTCATGGTT ACATTGAAGA GGCCTGCATC ATCCCTTGTC CTTCTGATTG CAAGCTCAGT
    GAGTGGTCCA ACTGGTCTCG CTGCAGTAAG TCCTGTGGAA GTGGTGTGAA GGTTCGGTCT
    AAATGGTTGC GTGAGAAACC ATATAATGGA GGGAGGCCTT GCCCCAAGCT GGACCATGTC
    AACCAGGCAC AGGTGTATGA GGTTGTCCCT TGCCACAGCG ACTGCAACCA GTACATATGG
    GTCACAGAGC CATGGAGCAT CTGCAAAGTG ACCTTTGTCA ACATGCGAGA TAACTGTGGA
    GAGGGTGTGC AAACCCGAAA AGTGAGATGC ATGCAGAACA CAGCAGATGG CCCTTCTGAC
    CATGTAGAAG ATTACCTCTG CGATCCAGAA GAGATGCCCC TGGGCTCTAG AGAGTGCAAA
    TTACCATGCC CTGAGGATTG TGTGATATCT GAATGGAGTC CATGGACCCG CTGCACTTTG
    CCTTGCAGTC AAAGCAGTTT CCGGCAAAGG TCAGCTGATC CCATCAGACA ACCTGCAGAT
    GAAGGAAGAG CCTGCCCTAA TGCTGTTGAG AAGGAACCCT GTAACCTAAA CAAAAACTGC
    TTCCACTATG ATTATAATGT AACAGACTGG AGTACGTGTC AGCTGAGTGA GAAGGCAGTT
    TGTGGAAACG GCATTAAAAC AAGGATGTTG GATTGTGTTC GAAGCGATGG CAAGTCGGTT
    GACCTGAAAT ATTGTGAAGA GCTTGGCCTG GAGAAGACCT GGCAGATGAA CACATCCTGC
    ATGGTAGAAT GTCCCGTCAA CTGCCAGCTT TCCGACTGGT CTCCTTGGTC AGAATGTTCT
    CAAACATGTG GCCTCACAGG GAAAATGATC CGAAGACGCA CAGTGACCCA GCCCTTTCAA
    GGTGATGGAC GGCCATGCCC TGCCCTGATG GAACAGTCCA AGCCTTGCCC AGTGAAGCCC
    TGTTATCAGT GGCAATATGG CCAGTGGTCT CTATGCCAAG TGCAGGATGC CCAGTGTGGC
    GAAGGGACGA GAACAAGGAA CATTTCTTGT GTAGTGAGTG ACGGGTCAAC TGATGATTTC
    AACAAAGTGG TGGATGAAGA ATTCTGTACT GACATTGAAC CCATAATAGA TGGTAATAAA
    AAAATGGTTC TCGAGGAAAC CTGCACCAAG CCTTGCCCAG GTGACTGTTA TCTGAAGGAC
    TGGTCTTCCT GGAGCCTGTG TCAGCTAACC TGTGTGAATG GTGAAGATCT AGGCTTTGGT
    GGAATACAGG TCCGTTCCAG AGCTGTGATT ATACAAGAGC TAGAAAATCA GCATCTGTGC
    CCAGAGCAAA TGTTAGAAAC AAAATCATGT AATGATGGAC AGTGTTATGA GTATAAATGG
    ATGGCCAGTG CTTGGAAGGG TTCTTCCCGA ACAGTCTGGT GTCAAAGGTC AGATGGTATA
    AATGTAACAG GGGGCTGCTT GGTGGTGCGC CAGCCTGATG CTGACAGGTC TTGTAACCCA
    CCGTGTAGTC AACCCCACTC GTACTGTAGT GAGATGAGGA CATGCAGTTG TGAAGAAGGG
    TATACTGAAG TCATGTCTTC TAACGGTACA CTTGAGCAAT GCACACTTAT CCCCGTGGTG
    GTGATACCCA CTGTGGAGGA CAAAAGAGGA GATGTGAAAA CCAGTCGGGC AGTACATCCA
    ACCCAATCCT CCAGTAATCC AACAGGACGG GGAAGGACCT GGTTTCTACA GCCATTTGGG
    CCAGATGGGA GACTAAAGAC CTGGGTTTAC GGTGTAGCAG CTGGGGCATT TGTGTTACTC
    ATCTTTATTG TCTCCATGAT TTATCTAGCT TGCAAAAAGC CAAAGAAACC CCAAAGAAGG
    CAAAACAACC GACTGAAACC TTTAACCCTA GCCTACGATG GAGATGCAGA CATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005628617.1
    CDS269..5305
    Translation

    Target ORF information:

    RefSeq Version XM_005628560.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris thrombospondin type 1 domain containing 7A (THSD7A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005628560.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGGGCTGC AAGCGAGGCG CTCGGCGTCC CAGAGCCCCG GCGCCCCGGG CCCGCGCCGG 
    GGTGTCCTGC AGCTGCTGCC GCTGCTCCTG CTGCTGCTGC TGCTGCTGCT GCTGCGCCCC
    GGAGCCTGCG GGGCTGCGGC TCCCGGCGGG GCTGAGGCAC CCACCCTCTA CCTCTGGAAG
    ACTGGTCCAT GGGGCCGGTG TATGGGAGAT GAATGTGGTC CAGGAGGCAT TCAGACCCGG
    GCCGTGTGGT GTGCTCATGT GGAAGGATGG ACCACATTAC ATACAAACTG TAAGCAGGCT
    GAGAGACCCA GCAACCAGCA AAACTGTTTC AAGGTGTGTG ACTGGCACAA GGAGTTGTAT
    GATTGGAGGC TGGGACCTTG GAACCAGTGT CAGCCTGTGA TATCCAAGAG CCTGGAGAAA
    CCCCCTGAGT GCATTAAGGG GGAGGAAGGC ATCCAGGTGA GAGAAATAAC GTGCATCCAG
    AAAGAGAAGG ACATTCCTGC AGAGGATATC ATTTGTGAGT ACTTTGAGCC CAAACCTCTC
    CTGGAACAGG CCTGCCTCAT CCCCTGCCAG CAAGATTGCA TCGTGTCTGA GTTTTCCGCG
    TGGTCAGAAT GTTCCAAGAC TTGTGGCAGT GGGCTCCAGC ACCGGACTCG GCATGTGGTG
    GCGCCTCCCC AGTTTGGAGG TTCTGGCTGC CCAAACTTGA CAGAGTTTCA GGTGTGCCAG
    TCCAGCCCCT GTGAAGATGA AGAGAGCATG TACAGCTTGC ACGTGGGTCC CTGGAGCTCA
    TGCTCGATGT CCCACTCAAG ACAAGTAAGA CAAGCAAGGA GACGTGGGAA GAATAAAGAA
    CGGGAGAAGG ACCGAGGAAA AGGAGTGAAG GATCCCGAAG CCCGTGAGCT TATTAAGAAA
    AAGAGAAACA GAAACAGACA GAATCGACAA GAGAACAAAT ATTGGGACAT CCAGATTGGG
    TATCAGACCA GAGAGGTTAT GTGTACTAAC AAGACGGGAA AAGCTGTTGA TTTGAGCTTT
    TGCCAGCAAG AGAAGCTGCC AATGACCTTC CAGTCCTGTG TGATCACCAA AGAGTGCCAG
    GTGTCAGAGT GGTCAGAATG GAGTCCCTGC TCAAAAACAT GCCATGACAT GACATCCCCC
    AGAGGCACTC GCGTCAGGAC GCGGATCATT AGGCAGTTTC CTATTGGCAG CGAGAAAGAG
    TGTCCAGAAC TTGAAGAAAA AGAACCCTGT GTGTCTCAAG GAGATGCAGT TGCCCCCTGT
    GCCACGTATG GCTGGAGAAC TACAGAGTGG GCGGAGTGCC GTGTGGACCC CTTGCTCAGT
    CAGCAGGACA AGAGGCGGGG CAACCAGACA GCCCTCTGTG GAGGAGGAGT CCAGACACGA
    GAGGTGTACT GCGTGCAGGC CAATGAAAAC CTCCTCTCAC ACTTAAACAC CCACAAGGAC
    AAAGAAGAAA GTAAATATCA GAGTACGTCG AAGTCTAGTG CTGCACCTTT CCTATCCATC
    ACTTTTGAAA TAACAGCCTC AAAACCAGTG GACTTGAAAT CATGCACTGG CCCTGTCCCT
    AATACTACAC AGCTGTGCCA CATTCCCTGT CCTATTGAAT GTGAGGTTTC ACCTTGGTCA
    GCTTGGGGAC CTTGTACTTA TGAAAACTGT AATGACCAGC AAGGCAAAAA AGGTTTCAAA
    CTGAGGAAGC GACGCATTAC CAATGAGCCC ACTGGAGGCT CTGGGGGCAC TGGGAACTGC
    CCTCACTTAC TGGAAGCCAT TCCCTGTGAA GAGCCATCCT GCTATGAATG GAAAGCAGTG
    AGGCTTGGAG ACTGTGAACC AGACAATGGA AAAGAGTGTG GCCCAGGCAC TCAAGTTCAG
    GAGGTTGTAT GCATCAACAG TGATGGAGAA GAAGTTGACA GACAGCTGTG CAGAGATGCC
    ATCTTCCCCA TCCCCGTTGC CTGTTATGCC CCATGCCCAA AGGACTGTGT GCTCAGCACG
    TGGTCTGCAT GGTCCTCCTG CTCACACACC TGCTCGGGGA AAACCACAGA AGGGAAACAG
    ATACGAGCAC GGTCCATTCT GGCCTATGCC GGTGACGAAG GTGGAGCTCA CTGTCCAAAT
    AGCAGTGCTT TGCAAGAGGT GCGCAGTTGT AACGAGCATC CCTGCACTGT GTACCACTGG
    CAGACTGGTC CCTGGGGCCA GTGCATAGAG GACACTTCAG TATCATCCTT CAACACGACG
    ACTACTTGGA ATGGAGAGGC GTCTTGCTCT GTTGGCATGC AGACAAGAAA AGTCATTTGT
    GTGCGGGTCA ATGTGGGCCA AGTAGGACCC AAAAAGTGTC CTGAAAGCCT TAGACCTGAA
    ACAGTGAGGC CCTGTCTGCT TCCTTGTAGA AAGGACTGTA TTGTGACACC ATATAGTGAC
    TGGACATCAT GCCCCAACTC ATGTAAAGAA GGGGACTCCG GAGTCAGAAA GCAGTCTCGG
    CACCGGGTCA TCATTCAGCA GCCGGCCAAC GGGGGCAGGG ACTGCACGGA CCCGCTCTAC
    GAAGAGAAAG TCTGTGAGGC CCCTCAAGTG TGCCAAAGCT ACAGGTGGAA GACCCACAAA
    TGGCGCAGGT GCCAGTTGGT CCCTTGGAGC GTGCGACAGG ACAGCCCTGG AGCACAGGAA
    GGCTGCGGAC CCGGACGCCA GGCAAGAGCT ATTACTTGTC GAAAGCAAGA TGGAGGACAG
    GCTGGCATCC ATGAATGCCT CTGGTATGCA GGCCCTGTGC CAGCCCTCAC CCAGGCCTGC
    CAGATCCCAT GCCAAGATGA CTGTCAGTTT ACCAGCTGGT CCAAGTTTTC TTCCTGCAAC
    GGAGACTGTG GTGCAGTTAG GACCAGAAAA CGCACTATTG TTGGAAAAAG TAAAAAGAAG
    GAAAAATGTA AAAATTCTCA TTTGTACCCC CTGATTGAGA CTCAGTACTG TCCTTGTGAC
    AAGTATAATG CACAGCCTGT GGGGAACTGG TCAGACTGCA TTTTACCAGA GGGGAAAGTG
    GAAGTGTTGC TTGGAGTGAA AACACAAGGA GATACCAAGG AATGTGGTCA AGGATATCGT
    TACCAAGCAA TGGCATGCTA CGACCAAAAT GGCAGGCTCG TGGAAACATC CAGATGTAAC
    AGTCATGGTT ACATTGAAGA GGCCTGCATC ATCCCTTGTC CTTCTGATTG CAAGCTCAGT
    GAGTGGTCCA ACTGGTCTCG CTGCAGTAAG TCCTGTGGAA GTGGTGTGAA GGTTCGGTCT
    AAATGGTTGC GTGAGAAACC ATATAATGGA GGGAGGCCTT GCCCCAAGCT GGACCATGTC
    AACCAGGCAC AGGTGTATGA GGTTGTCCCT TGCCACAGCG ACTGCAACCA GTACATATGG
    GTCACAGAGC CATGGAGCAT CTGCAAAGTG ACCTTTGTCA ACATGCGAGA TAACTGTGGA
    GAGGGTGTGC AAACCCGAAA AGTGAGATGC ATGCAGAACA CAGCAGATGG CCCTTCTGAC
    CATGTAGAAG ATTACCTCTG CGATCCAGAA GAGATGCCCC TGGGCTCTAG AGAGTGCAAA
    TTACCATGCC CTGAGGATTG TGTGATATCT GAATGGAGTC CATGGACCCG CTGCACTTTG
    CCTTGCAGTC AAAGCAGTTT CCGGCAAAGG TCAGCTGATC CCATCAGACA ACCTGCAGAT
    GAAGGAAGAG CCTGCCCTAA TGCTGTTGAG AAGGAACCCT GTAACCTAAA CAAAAACTGC
    TTCCACTATG ATTATAATGT AACAGACTGG AGTACGTGTC AGCTGAGTGA GAAGGCAGTT
    TGTGGAAACG GCATTAAAAC AAGGATGTTG GATTGTGTTC GAAGCGATGG CAAGTCGGTT
    GACCTGAAAT ATTGTGAAGA GCTTGGCCTG GAGAAGACCT GGCAGATGAA CACATCCTGC
    ATGGTAGAAT GTCCCGTCAA CTGCCAGCTT TCCGACTGGT CTCCTTGGTC AGAATGTTCT
    CAAACATGTG GCCTCACAGG GAAAATGATC CGAAGACGCA CAGTGACCCA GCCCTTTCAA
    GGTGATGGAC GGCCATGCCC TGCCCTGATG GAACAGTCCA AGCCTTGCCC AGTGAAGCCC
    TGTTATCAGT GGCAATATGG CCAGTGGTCT CTATGCCAAG TGCAGGATGC CCAGTGTGGC
    GAAGGGACGA GAACAAGGAA CATTTCTTGT GTAGTGAGTG ACGGGTCAAC TGATGATTTC
    AACAAAGTGG TGGATGAAGA ATTCTGTACT GACATTGAAC CCATAATAGA TGGTAATAAA
    AAAATGGTTC TCGAGGAAAC CTGCACCAAG CCTTGCCCAG GTGACTGTTA TCTGAAGGAC
    TGGTCTTCCT GGAGCCTGTG TCAGCTAACC TGTGTGAATG GTGAAGATCT AGGCTTTGGT
    GGAATACAGG TCCGTTCCAG AGCTGTGATT ATACAAGAGC TAGAAAATCA GCATCTGTGC
    CCAGAGCAAA TGTTAGAAAC AAAATCATGT AATGATGGAC AGTGTTATGA GTATAAATGG
    ATGGCCAGTG CTTGGAAGGG TTCTTCCCGA ACAGTCTGGT GTCAAAGGTC AGATGGTATA
    AATGTAACAG GGGGCTGCTT GGTGGTGCGC CAGCCTGATG CTGACAGGTC TTGTAACCCA
    CCGTGTAGTC AACCCCACTC GTACTGTAGT GAGATGAGGA CATGCAGTTG TGAAGAAGGG
    TATACTGAAG TCATGTCTTC TAACGGTACA CTTGAGCAAT GCACACTTAT CCCCGTGGTG
    GTGATACCCA CTGTGGAGGA CAAAAGAGGA GATGTGAAAA CCAGTCGGGC AGTACATCCA
    ACCCAATCCT CCAGTAATCC AACAGGACGG GGAAGGACCT GGTTTCTACA GCCATTTGGG
    CCAGATGGGA GACTAAAGAC CTGGGTTTAC GGTGTAGCAG CTGGGGCATT TGTGTTACTC
    ATCTTTATTG TCTCCATGAT TTATCTAGCT TGCAAAAAGC CAAAGAAACC CCAAAGAAGG
    CAAAACAACC GACTGAAACC TTTAACCCTA GCCTACGATG GAGATGCAGA CATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe16746
    Clone ID Related Accession (Same CDS sequence) XM_005628560.3 , XM_005628560.2
    Accession Version XM_005628560.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5037bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product thrombospondin type-1 domain-containing protein 7A isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726084.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005628560.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGGGCTGC AAGCGAGGCG CTCGGCGTCC CAGAGCCCCG GCGCCCCGGG CCCGCGCCGG 
    GGTGTCCTGC AGCTGCTGCC GCTGCTCCTG CTGCTGCTGC TGCTGCTGCT GCTGCGCCCC
    GGAGCCTGCG GGGCTGCGGC TCCCGGCGGG GCTGAGGCAC CCACCCTCTA CCTCTGGAAG
    ACTGGTCCAT GGGGCCGGTG TATGGGAGAT GAATGTGGTC CAGGAGGCAT TCAGACCCGG
    GCCGTGTGGT GTGCTCATGT GGAAGGATGG ACCACATTAC ATACAAACTG TAAGCAGGCT
    GAGAGACCCA GCAACCAGCA AAACTGTTTC AAGGTGTGTG ACTGGCACAA GGAGTTGTAT
    GATTGGAGGC TGGGACCTTG GAACCAGTGT CAGCCTGTGA TATCCAAGAG CCTGGAGAAA
    CCCCCTGAGT GCATTAAGGG GGAGGAAGGC ATCCAGGTGA GAGAAATAAC GTGCATCCAG
    AAAGAGAAGG ACATTCCTGC AGAGGATATC ATTTGTGAGT ACTTTGAGCC CAAACCTCTC
    CTGGAACAGG CCTGCCTCAT CCCCTGCCAG CAAGATTGCA TCGTGTCTGA GTTTTCCGCG
    TGGTCAGAAT GTTCCAAGAC TTGTGGCAGT GGGCTCCAGC ACCGGACTCG GCATGTGGTG
    GCGCCTCCCC AGTTTGGAGG TTCTGGCTGC CCAAACTTGA CAGAGTTTCA GGTGTGCCAG
    TCCAGCCCCT GTGAAGATGA AGAGAGCATG TACAGCTTGC ACGTGGGTCC CTGGAGCTCA
    TGCTCGATGT CCCACTCAAG ACAAGTAAGA CAAGCAAGGA GACGTGGGAA GAATAAAGAA
    CGGGAGAAGG ACCGAGGAAA AGGAGTGAAG GATCCCGAAG CCCGTGAGCT TATTAAGAAA
    AAGAGAAACA GAAACAGACA GAATCGACAA GAGAACAAAT ATTGGGACAT CCAGATTGGG
    TATCAGACCA GAGAGGTTAT GTGTACTAAC AAGACGGGAA AAGCTGTTGA TTTGAGCTTT
    TGCCAGCAAG AGAAGCTGCC AATGACCTTC CAGTCCTGTG TGATCACCAA AGAGTGCCAG
    GTGTCAGAGT GGTCAGAATG GAGTCCCTGC TCAAAAACAT GCCATGACAT GACATCCCCC
    AGAGGCACTC GCGTCAGGAC GCGGATCATT AGGCAGTTTC CTATTGGCAG CGAGAAAGAG
    TGTCCAGAAC TTGAAGAAAA AGAACCCTGT GTGTCTCAAG GAGATGCAGT TGCCCCCTGT
    GCCACGTATG GCTGGAGAAC TACAGAGTGG GCGGAGTGCC GTGTGGACCC CTTGCTCAGT
    CAGCAGGACA AGAGGCGGGG CAACCAGACA GCCCTCTGTG GAGGAGGAGT CCAGACACGA
    GAGGTGTACT GCGTGCAGGC CAATGAAAAC CTCCTCTCAC ACTTAAACAC CCACAAGGAC
    AAAGAAGAAA GTAAATATCA GAGTACGTCG AAGTCTAGTG CTGCACCTTT CCTATCCATC
    ACTTTTGAAA TAACAGCCTC AAAACCAGTG GACTTGAAAT CATGCACTGG CCCTGTCCCT
    AATACTACAC AGCTGTGCCA CATTCCCTGT CCTATTGAAT GTGAGGTTTC ACCTTGGTCA
    GCTTGGGGAC CTTGTACTTA TGAAAACTGT AATGACCAGC AAGGCAAAAA AGGTTTCAAA
    CTGAGGAAGC GACGCATTAC CAATGAGCCC ACTGGAGGCT CTGGGGGCAC TGGGAACTGC
    CCTCACTTAC TGGAAGCCAT TCCCTGTGAA GAGCCATCCT GCTATGAATG GAAAGCAGTG
    AGGCTTGGAG ACTGTGAACC AGACAATGGA AAAGAGTGTG GCCCAGGCAC TCAAGTTCAG
    GAGGTTGTAT GCATCAACAG TGATGGAGAA GAAGTTGACA GACAGCTGTG CAGAGATGCC
    ATCTTCCCCA TCCCCGTTGC CTGTTATGCC CCATGCCCAA AGGACTGTGT GCTCAGCACG
    TGGTCTGCAT GGTCCTCCTG CTCACACACC TGCTCGGGGA AAACCACAGA AGGGAAACAG
    ATACGAGCAC GGTCCATTCT GGCCTATGCC GGTGACGAAG GTGGAGCTCA CTGTCCAAAT
    AGCAGTGCTT TGCAAGAGGT GCGCAGTTGT AACGAGCATC CCTGCACTGT GTACCACTGG
    CAGACTGGTC CCTGGGGCCA GTGCATAGAG GACACTTCAG TATCATCCTT CAACACGACG
    ACTACTTGGA ATGGAGAGGC GTCTTGCTCT GTTGGCATGC AGACAAGAAA AGTCATTTGT
    GTGCGGGTCA ATGTGGGCCA AGTAGGACCC AAAAAGTGTC CTGAAAGCCT TAGACCTGAA
    ACAGTGAGGC CCTGTCTGCT TCCTTGTAGA AAGGACTGTA TTGTGACACC ATATAGTGAC
    TGGACATCAT GCCCCAACTC ATGTAAAGAA GGGGACTCCG GAGTCAGAAA GCAGTCTCGG
    CACCGGGTCA TCATTCAGCA GCCGGCCAAC GGGGGCAGGG ACTGCACGGA CCCGCTCTAC
    GAAGAGAAAG TCTGTGAGGC CCCTCAAGTG TGCCAAAGCT ACAGGTGGAA GACCCACAAA
    TGGCGCAGGT GCCAGTTGGT CCCTTGGAGC GTGCGACAGG ACAGCCCTGG AGCACAGGAA
    GGCTGCGGAC CCGGACGCCA GGCAAGAGCT ATTACTTGTC GAAAGCAAGA TGGAGGACAG
    GCTGGCATCC ATGAATGCCT CTGGTATGCA GGCCCTGTGC CAGCCCTCAC CCAGGCCTGC
    CAGATCCCAT GCCAAGATGA CTGTCAGTTT ACCAGCTGGT CCAAGTTTTC TTCCTGCAAC
    GGAGACTGTG GTGCAGTTAG GACCAGAAAA CGCACTATTG TTGGAAAAAG TAAAAAGAAG
    GAAAAATGTA AAAATTCTCA TTTGTACCCC CTGATTGAGA CTCAGTACTG TCCTTGTGAC
    AAGTATAATG CACAGCCTGT GGGGAACTGG TCAGACTGCA TTTTACCAGA GGGGAAAGTG
    GAAGTGTTGC TTGGAGTGAA AACACAAGGA GATACCAAGG AATGTGGTCA AGGATATCGT
    TACCAAGCAA TGGCATGCTA CGACCAAAAT GGCAGGCTCG TGGAAACATC CAGATGTAAC
    AGTCATGGTT ACATTGAAGA GGCCTGCATC ATCCCTTGTC CTTCTGATTG CAAGCTCAGT
    GAGTGGTCCA ACTGGTCTCG CTGCAGTAAG TCCTGTGGAA GTGGTGTGAA GGTTCGGTCT
    AAATGGTTGC GTGAGAAACC ATATAATGGA GGGAGGCCTT GCCCCAAGCT GGACCATGTC
    AACCAGGCAC AGGTGTATGA GGTTGTCCCT TGCCACAGCG ACTGCAACCA GTACATATGG
    GTCACAGAGC CATGGAGCAT CTGCAAAGTG ACCTTTGTCA ACATGCGAGA TAACTGTGGA
    GAGGGTGTGC AAACCCGAAA AGTGAGATGC ATGCAGAACA CAGCAGATGG CCCTTCTGAC
    CATGTAGAAG ATTACCTCTG CGATCCAGAA GAGATGCCCC TGGGCTCTAG AGAGTGCAAA
    TTACCATGCC CTGAGGATTG TGTGATATCT GAATGGAGTC CATGGACCCG CTGCACTTTG
    CCTTGCAGTC AAAGCAGTTT CCGGCAAAGG TCAGCTGATC CCATCAGACA ACCTGCAGAT
    GAAGGAAGAG CCTGCCCTAA TGCTGTTGAG AAGGAACCCT GTAACCTAAA CAAAAACTGC
    TTCCACTATG ATTATAATGT AACAGACTGG AGTACGTGTC AGCTGAGTGA GAAGGCAGTT
    TGTGGAAACG GCATTAAAAC AAGGATGTTG GATTGTGTTC GAAGCGATGG CAAGTCGGTT
    GACCTGAAAT ATTGTGAAGA GCTTGGCCTG GAGAAGACCT GGCAGATGAA CACATCCTGC
    ATGGTAGAAT GTCCCGTCAA CTGCCAGCTT TCCGACTGGT CTCCTTGGTC AGAATGTTCT
    CAAACATGTG GCCTCACAGG GAAAATGATC CGAAGACGCA CAGTGACCCA GCCCTTTCAA
    GGTGATGGAC GGCCATGCCC TGCCCTGATG GAACAGTCCA AGCCTTGCCC AGTGAAGCCC
    TGTTATCAGT GGCAATATGG CCAGTGGTCT CTATGCCAAG TGCAGGATGC CCAGTGTGGC
    GAAGGGACGA GAACAAGGAA CATTTCTTGT GTAGTGAGTG ACGGGTCAAC TGATGATTTC
    AACAAAGTGG TGGATGAAGA ATTCTGTACT GACATTGAAC CCATAATAGA TGGTAATAAA
    AAAATGGTTC TCGAGGAAAC CTGCACCAAG CCTTGCCCAG GTGACTGTTA TCTGAAGGAC
    TGGTCTTCCT GGAGCCTGTG TCAGCTAACC TGTGTGAATG GTGAAGATCT AGGCTTTGGT
    GGAATACAGG TCCGTTCCAG AGCTGTGATT ATACAAGAGC TAGAAAATCA GCATCTGTGC
    CCAGAGCAAA TGTTAGAAAC AAAATCATGT AATGATGGAC AGTGTTATGA GTATAAATGG
    ATGGCCAGTG CTTGGAAGGG TTCTTCCCGA ACAGTCTGGT GTCAAAGGTC AGATGGTATA
    AATGTAACAG GGGGCTGCTT GGTGGTGCGC CAGCCTGATG CTGACAGGTC TTGTAACCCA
    CCGTGTAGTC AACCCCACTC GTACTGTAGT GAGATGAGGA CATGCAGTTG TGAAGAAGGG
    TATACTGAAG TCATGTCTTC TAACGGTACA CTTGAGCAAT GCACACTTAT CCCCGTGGTG
    GTGATACCCA CTGTGGAGGA CAAAAGAGGA GATGTGAAAA CCAGTCGGGC AGTACATCCA
    ACCCAATCCT CCAGTAATCC AACAGGACGG GGAAGGACCT GGTTTCTACA GCCATTTGGG
    CCAGATGGGA GACTAAAGAC CTGGGTTTAC GGTGTAGCAG CTGGGGCATT TGTGTTACTC
    ATCTTTATTG TCTCCATGAT TTATCTAGCT TGCAAAAAGC CAAAGAAACC CCAAAGAAGG
    CAAAACAACC GACTGAAACC TTTAACCCTA GCCTACGATG GAGATGCAGA CATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005628617.1
    CDS343..5379
    Translation

    Target ORF information:

    RefSeq Version XM_005628560.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris thrombospondin, type I, domain containing 7A (THSD7A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005628560.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGGGCTGC AAGCGAGGCG CTCGGCGTCC CAGAGCCCCG GCGCCCCGGG CCCGCGCCGG 
    GGTGTCCTGC AGCTGCTGCC GCTGCTCCTG CTGCTGCTGC TGCTGCTGCT GCTGCGCCCC
    GGAGCCTGCG GGGCTGCGGC TCCCGGCGGG GCTGAGGCAC CCACCCTCTA CCTCTGGAAG
    ACTGGTCCAT GGGGCCGGTG TATGGGAGAT GAATGTGGTC CAGGAGGCAT TCAGACCCGG
    GCCGTGTGGT GTGCTCATGT GGAAGGATGG ACCACATTAC ATACAAACTG TAAGCAGGCT
    GAGAGACCCA GCAACCAGCA AAACTGTTTC AAGGTGTGTG ACTGGCACAA GGAGTTGTAT
    GATTGGAGGC TGGGACCTTG GAACCAGTGT CAGCCTGTGA TATCCAAGAG CCTGGAGAAA
    CCCCCTGAGT GCATTAAGGG GGAGGAAGGC ATCCAGGTGA GAGAAATAAC GTGCATCCAG
    AAAGAGAAGG ACATTCCTGC AGAGGATATC ATTTGTGAGT ACTTTGAGCC CAAACCTCTC
    CTGGAACAGG CCTGCCTCAT CCCCTGCCAG CAAGATTGCA TCGTGTCTGA GTTTTCCGCG
    TGGTCAGAAT GTTCCAAGAC TTGTGGCAGT GGGCTCCAGC ACCGGACTCG GCATGTGGTG
    GCGCCTCCCC AGTTTGGAGG TTCTGGCTGC CCAAACTTGA CAGAGTTTCA GGTGTGCCAG
    TCCAGCCCCT GTGAAGATGA AGAGAGCATG TACAGCTTGC ACGTGGGTCC CTGGAGCTCA
    TGCTCGATGT CCCACTCAAG ACAAGTAAGA CAAGCAAGGA GACGTGGGAA GAATAAAGAA
    CGGGAGAAGG ACCGAGGAAA AGGAGTGAAG GATCCCGAAG CCCGTGAGCT TATTAAGAAA
    AAGAGAAACA GAAACAGACA GAATCGACAA GAGAACAAAT ATTGGGACAT CCAGATTGGG
    TATCAGACCA GAGAGGTTAT GTGTACTAAC AAGACGGGAA AAGCTGTTGA TTTGAGCTTT
    TGCCAGCAAG AGAAGCTGCC AATGACCTTC CAGTCCTGTG TGATCACCAA AGAGTGCCAG
    GTGTCAGAGT GGTCAGAATG GAGTCCCTGC TCAAAAACAT GCCATGACAT GACATCCCCC
    AGAGGCACTC GCGTCAGGAC GCGGATCATT AGGCAGTTTC CTATTGGCAG CGAGAAAGAG
    TGTCCAGAAC TTGAAGAAAA AGAACCCTGT GTGTCTCAAG GAGATGCAGT TGCCCCCTGT
    GCCACGTATG GCTGGAGAAC TACAGAGTGG GCGGAGTGCC GTGTGGACCC CTTGCTCAGT
    CAGCAGGACA AGAGGCGGGG CAACCAGACA GCCCTCTGTG GAGGAGGAGT CCAGACACGA
    GAGGTGTACT GCGTGCAGGC CAATGAAAAC CTCCTCTCAC ACTTAAACAC CCACAAGGAC
    AAAGAAGAAA GTAAATATCA GAGTACGTCG AAGTCTAGTG CTGCACCTTT CCTATCCATC
    ACTTTTGAAA TAACAGCCTC AAAACCAGTG GACTTGAAAT CATGCACTGG CCCTGTCCCT
    AATACTACAC AGCTGTGCCA CATTCCCTGT CCTATTGAAT GTGAGGTTTC ACCTTGGTCA
    GCTTGGGGAC CTTGTACTTA TGAAAACTGT AATGACCAGC AAGGCAAAAA AGGTTTCAAA
    CTGAGGAAGC GACGCATTAC CAATGAGCCC ACTGGAGGCT CTGGGGGCAC TGGGAACTGC
    CCTCACTTAC TGGAAGCCAT TCCCTGTGAA GAGCCATCCT GCTATGAATG GAAAGCAGTG
    AGGCTTGGAG ACTGTGAACC AGACAATGGA AAAGAGTGTG GCCCAGGCAC TCAAGTTCAG
    GAGGTTGTAT GCATCAACAG TGATGGAGAA GAAGTTGACA GACAGCTGTG CAGAGATGCC
    ATCTTCCCCA TCCCCGTTGC CTGTTATGCC CCATGCCCAA AGGACTGTGT GCTCAGCACG
    TGGTCTGCAT GGTCCTCCTG CTCACACACC TGCTCGGGGA AAACCACAGA AGGGAAACAG
    ATACGAGCAC GGTCCATTCT GGCCTATGCC GGTGACGAAG GTGGAGCTCA CTGTCCAAAT
    AGCAGTGCTT TGCAAGAGGT GCGCAGTTGT AACGAGCATC CCTGCACTGT GTACCACTGG
    CAGACTGGTC CCTGGGGCCA GTGCATAGAG GACACTTCAG TATCATCCTT CAACACGACG
    ACTACTTGGA ATGGAGAGGC GTCTTGCTCT GTTGGCATGC AGACAAGAAA AGTCATTTGT
    GTGCGGGTCA ATGTGGGCCA AGTAGGACCC AAAAAGTGTC CTGAAAGCCT TAGACCTGAA
    ACAGTGAGGC CCTGTCTGCT TCCTTGTAGA AAGGACTGTA TTGTGACACC ATATAGTGAC
    TGGACATCAT GCCCCAACTC ATGTAAAGAA GGGGACTCCG GAGTCAGAAA GCAGTCTCGG
    CACCGGGTCA TCATTCAGCA GCCGGCCAAC GGGGGCAGGG ACTGCACGGA CCCGCTCTAC
    GAAGAGAAAG TCTGTGAGGC CCCTCAAGTG TGCCAAAGCT ACAGGTGGAA GACCCACAAA
    TGGCGCAGGT GCCAGTTGGT CCCTTGGAGC GTGCGACAGG ACAGCCCTGG AGCACAGGAA
    GGCTGCGGAC CCGGACGCCA GGCAAGAGCT ATTACTTGTC GAAAGCAAGA TGGAGGACAG
    GCTGGCATCC ATGAATGCCT CTGGTATGCA GGCCCTGTGC CAGCCCTCAC CCAGGCCTGC
    CAGATCCCAT GCCAAGATGA CTGTCAGTTT ACCAGCTGGT CCAAGTTTTC TTCCTGCAAC
    GGAGACTGTG GTGCAGTTAG GACCAGAAAA CGCACTATTG TTGGAAAAAG TAAAAAGAAG
    GAAAAATGTA AAAATTCTCA TTTGTACCCC CTGATTGAGA CTCAGTACTG TCCTTGTGAC
    AAGTATAATG CACAGCCTGT GGGGAACTGG TCAGACTGCA TTTTACCAGA GGGGAAAGTG
    GAAGTGTTGC TTGGAGTGAA AACACAAGGA GATACCAAGG AATGTGGTCA AGGATATCGT
    TACCAAGCAA TGGCATGCTA CGACCAAAAT GGCAGGCTCG TGGAAACATC CAGATGTAAC
    AGTCATGGTT ACATTGAAGA GGCCTGCATC ATCCCTTGTC CTTCTGATTG CAAGCTCAGT
    GAGTGGTCCA ACTGGTCTCG CTGCAGTAAG TCCTGTGGAA GTGGTGTGAA GGTTCGGTCT
    AAATGGTTGC GTGAGAAACC ATATAATGGA GGGAGGCCTT GCCCCAAGCT GGACCATGTC
    AACCAGGCAC AGGTGTATGA GGTTGTCCCT TGCCACAGCG ACTGCAACCA GTACATATGG
    GTCACAGAGC CATGGAGCAT CTGCAAAGTG ACCTTTGTCA ACATGCGAGA TAACTGTGGA
    GAGGGTGTGC AAACCCGAAA AGTGAGATGC ATGCAGAACA CAGCAGATGG CCCTTCTGAC
    CATGTAGAAG ATTACCTCTG CGATCCAGAA GAGATGCCCC TGGGCTCTAG AGAGTGCAAA
    TTACCATGCC CTGAGGATTG TGTGATATCT GAATGGAGTC CATGGACCCG CTGCACTTTG
    CCTTGCAGTC AAAGCAGTTT CCGGCAAAGG TCAGCTGATC CCATCAGACA ACCTGCAGAT
    GAAGGAAGAG CCTGCCCTAA TGCTGTTGAG AAGGAACCCT GTAACCTAAA CAAAAACTGC
    TTCCACTATG ATTATAATGT AACAGACTGG AGTACGTGTC AGCTGAGTGA GAAGGCAGTT
    TGTGGAAACG GCATTAAAAC AAGGATGTTG GATTGTGTTC GAAGCGATGG CAAGTCGGTT
    GACCTGAAAT ATTGTGAAGA GCTTGGCCTG GAGAAGACCT GGCAGATGAA CACATCCTGC
    ATGGTAGAAT GTCCCGTCAA CTGCCAGCTT TCCGACTGGT CTCCTTGGTC AGAATGTTCT
    CAAACATGTG GCCTCACAGG GAAAATGATC CGAAGACGCA CAGTGACCCA GCCCTTTCAA
    GGTGATGGAC GGCCATGCCC TGCCCTGATG GAACAGTCCA AGCCTTGCCC AGTGAAGCCC
    TGTTATCAGT GGCAATATGG CCAGTGGTCT CTATGCCAAG TGCAGGATGC CCAGTGTGGC
    GAAGGGACGA GAACAAGGAA CATTTCTTGT GTAGTGAGTG ACGGGTCAAC TGATGATTTC
    AACAAAGTGG TGGATGAAGA ATTCTGTACT GACATTGAAC CCATAATAGA TGGTAATAAA
    AAAATGGTTC TCGAGGAAAC CTGCACCAAG CCTTGCCCAG GTGACTGTTA TCTGAAGGAC
    TGGTCTTCCT GGAGCCTGTG TCAGCTAACC TGTGTGAATG GTGAAGATCT AGGCTTTGGT
    GGAATACAGG TCCGTTCCAG AGCTGTGATT ATACAAGAGC TAGAAAATCA GCATCTGTGC
    CCAGAGCAAA TGTTAGAAAC AAAATCATGT AATGATGGAC AGTGTTATGA GTATAAATGG
    ATGGCCAGTG CTTGGAAGGG TTCTTCCCGA ACAGTCTGGT GTCAAAGGTC AGATGGTATA
    AATGTAACAG GGGGCTGCTT GGTGGTGCGC CAGCCTGATG CTGACAGGTC TTGTAACCCA
    CCGTGTAGTC AACCCCACTC GTACTGTAGT GAGATGAGGA CATGCAGTTG TGAAGAAGGG
    TATACTGAAG TCATGTCTTC TAACGGTACA CTTGAGCAAT GCACACTTAT CCCCGTGGTG
    GTGATACCCA CTGTGGAGGA CAAAAGAGGA GATGTGAAAA CCAGTCGGGC AGTACATCCA
    ACCCAATCCT CCAGTAATCC AACAGGACGG GGAAGGACCT GGTTTCTACA GCCATTTGGG
    CCAGATGGGA GACTAAAGAC CTGGGTTTAC GGTGTAGCAG CTGGGGCATT TGTGTTACTC
    ATCTTTATTG TCTCCATGAT TTATCTAGCT TGCAAAAAGC CAAAGAAACC CCAAAGAAGG
    CAAAACAACC GACTGAAACC TTTAACCCTA GCCTACGATG GAGATGCAGA CATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe16747
    Clone ID Related Accession (Same CDS sequence) XM_003431865.5 , XM_003431865.4
    Accession Version XM_003431865.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4968bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product thrombospondin type-1 domain-containing protein 7A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006596.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003431865.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGCTGC AAGCGAGGCG CTCGGCGTCC CAGAGCCCCG GCGCCCCGGG CCCGCGCCGG 
    GGTGTCCTGC AGCTGCTGCC GCTGCTCCTG CTGCTGCTGC TGCTGCTGCT GCTGCGCCCC
    GGAGCCTGCG GGGCTGCGGC TCCCGGCGGG GCTGAGGCAC CCACCCTCTA CCTCTGGAAG
    ACTGGTCCAT GGGGCCGGTG TATGGGAGAT GAATGTGGTC CAGGAGGCAT TCAGACCCGG
    GCCGTGTGGT GTGCTCATGT GGAAGGATGG ACCACATTAC ATACAAACTG TAAGCAGGCT
    GAGAGACCCA GCAACCAGCA AAACTGTTTC AAGGTGTGTG ACTGGCACAA GGAGTTGTAT
    GATTGGAGGC TGGGACCTTG GAACCAGTGT CAGCCTGTGA TATCCAAGAG CCTGGAGAAA
    CCCCCTGAGT GCATTAAGGG GGAGGAAGGC ATCCAGGTGA GAGAAATAAC GTGCATCCAG
    AAAGAGAAGG ACATTCCTGC AGAGGATATC ATTTGTGAGT ACTTTGAGCC CAAACCTCTC
    CTGGAACAGG CCTGCCTCAT CCCCTGCCAG CAAGATTGCA TCGTGTCTGA GTTTTCCGCG
    TGGTCAGAAT GTTCCAAGAC TTGTGGCAGT GGGCTCCAGC ACCGGACTCG GCATGTGGTG
    GCGCCTCCCC AGTTTGGAGG TTCTGGCTGC CCAAACTTGA CAGAGTTTCA GGTGTGCCAG
    TCCAGCCCCT GTGAAGATGA AGAGAGCATG TACAGCTTGC ACGTGGGTCC CTGGAGCTCA
    TGCTCGATGT CCCACTCAAG ACAAGTAAGA CAAGCAAGGA GACGTGGGAA GAATAAAGAA
    CGGGAGAAGG ACCGAGGAAA AGGAGTGAAG GATCCCGAAG CCCGTGAGCT TATTAAGAAA
    AAGAGAAACA GAAACAGACA GAATCGACAA GAGAACAAAT ATTGGGACAT CCAGATTGGG
    TATCAGACCA GAGAGGTTAT GTGTACTAAC AAGACGGGAA AAGCTGTTGA TTTGAGCTTT
    TGCCAGCAAG AGAAGCTGCC AATGACCTTC CAGTCCTGTG TGATCACCAA AGAGTGCCAG
    GTGTCAGAGT GGTCAGAATG GAGTCCCTGC TCAAAAACAT GCCATGACAT GACATCCCCC
    AGAGGCACTC GCGTCAGGAC GCGGATCATT AGGCAGTTTC CTATTGGCAG CGAGAAAGAG
    TGTCCAGAAC TTGAAGAAAA AGAACCCTGT GTGTCTCAAG GAGATGCAGT TGCCCCCTGT
    GCCACGTATG GCTGGAGAAC TACAGAGTGG GCGGAGTGCC GTGTGGACCC CTTGCTCAGT
    CAGCAGGACA AGAGGCGGGG CAACCAGACA GCCCTCTGTG GAGGAGGAGT CCAGACACGA
    GAGGTGTACT GCGTGCAGGC CAATGAAAAC CTCCTCTCAC ACTTAAACAC CCACAAGGAC
    AAAGAAGCCT CAAAACCAGT GGACTTGAAA TCATGCACTG GCCCTGTCCC TAATACTACA
    CAGCTGTGCC ACATTCCCTG TCCTATTGAA TGTGAGGTTT CACCTTGGTC AGCTTGGGGA
    CCTTGTACTT ATGAAAACTG TAATGACCAG CAAGGCAAAA AAGGTTTCAA ACTGAGGAAG
    CGACGCATTA CCAATGAGCC CACTGGAGGC TCTGGGGGCA CTGGGAACTG CCCTCACTTA
    CTGGAAGCCA TTCCCTGTGA AGAGCCATCC TGCTATGAAT GGAAAGCAGT GAGGCTTGGA
    GACTGTGAAC CAGACAATGG AAAAGAGTGT GGCCCAGGCA CTCAAGTTCA GGAGGTTGTA
    TGCATCAACA GTGATGGAGA AGAAGTTGAC AGACAGCTGT GCAGAGATGC CATCTTCCCC
    ATCCCCGTTG CCTGTTATGC CCCATGCCCA AAGGACTGTG TGCTCAGCAC GTGGTCTGCA
    TGGTCCTCCT GCTCACACAC CTGCTCGGGG AAAACCACAG AAGGGAAACA GATACGAGCA
    CGGTCCATTC TGGCCTATGC CGGTGACGAA GGTGGAGCTC ACTGTCCAAA TAGCAGTGCT
    TTGCAAGAGG TGCGCAGTTG TAACGAGCAT CCCTGCACTG TGTACCACTG GCAGACTGGT
    CCCTGGGGCC AGTGCATAGA GGACACTTCA GTATCATCCT TCAACACGAC GACTACTTGG
    AATGGAGAGG CGTCTTGCTC TGTTGGCATG CAGACAAGAA AAGTCATTTG TGTGCGGGTC
    AATGTGGGCC AAGTAGGACC CAAAAAGTGT CCTGAAAGCC TTAGACCTGA AACAGTGAGG
    CCCTGTCTGC TTCCTTGTAG AAAGGACTGT ATTGTGACAC CATATAGTGA CTGGACATCA
    TGCCCCAACT CATGTAAAGA AGGGGACTCC GGAGTCAGAA AGCAGTCTCG GCACCGGGTC
    ATCATTCAGC AGCCGGCCAA CGGGGGCAGG GACTGCACGG ACCCGCTCTA CGAAGAGAAA
    GTCTGTGAGG CCCCTCAAGT GTGCCAAAGC TACAGGTGGA AGACCCACAA ATGGCGCAGG
    TGCCAGTTGG TCCCTTGGAG CGTGCGACAG GACAGCCCTG GAGCACAGGA AGGCTGCGGA
    CCCGGACGCC AGGCAAGAGC TATTACTTGT CGAAAGCAAG ATGGAGGACA GGCTGGCATC
    CATGAATGCC TCTGGTATGC AGGCCCTGTG CCAGCCCTCA CCCAGGCCTG CCAGATCCCA
    TGCCAAGATG ACTGTCAGTT TACCAGCTGG TCCAAGTTTT CTTCCTGCAA CGGAGACTGT
    GGTGCAGTTA GGACCAGAAA ACGCACTATT GTTGGAAAAA GTAAAAAGAA GGAAAAATGT
    AAAAATTCTC ATTTGTACCC CCTGATTGAG ACTCAGTACT GTCCTTGTGA CAAGTATAAT
    GCACAGCCTG TGGGGAACTG GTCAGACTGC ATTTTACCAG AGGGGAAAGT GGAAGTGTTG
    CTTGGAGTGA AAACACAAGG AGATACCAAG GAATGTGGTC AAGGATATCG TTACCAAGCA
    ATGGCATGCT ACGACCAAAA TGGCAGGCTC GTGGAAACAT CCAGATGTAA CAGTCATGGT
    TACATTGAAG AGGCCTGCAT CATCCCTTGT CCTTCTGATT GCAAGCTCAG TGAGTGGTCC
    AACTGGTCTC GCTGCAGTAA GTCCTGTGGA AGTGGTGTGA AGGTTCGGTC TAAATGGTTG
    CGTGAGAAAC CATATAATGG AGGGAGGCCT TGCCCCAAGC TGGACCATGT CAACCAGGCA
    CAGGTGTATG AGGTTGTCCC TTGCCACAGC GACTGCAACC AGTACATATG GGTCACAGAG
    CCATGGAGCA TCTGCAAAGT GACCTTTGTC AACATGCGAG ATAACTGTGG AGAGGGTGTG
    CAAACCCGAA AAGTGAGATG CATGCAGAAC ACAGCAGATG GCCCTTCTGA CCATGTAGAA
    GATTACCTCT GCGATCCAGA AGAGATGCCC CTGGGCTCTA GAGAGTGCAA ATTACCATGC
    CCTGAGGATT GTGTGATATC TGAATGGAGT CCATGGACCC GCTGCACTTT GCCTTGCAGT
    CAAAGCAGTT TCCGGCAAAG GTCAGCTGAT CCCATCAGAC AACCTGCAGA TGAAGGAAGA
    GCCTGCCCTA ATGCTGTTGA GAAGGAACCC TGTAACCTAA ACAAAAACTG CTTCCACTAT
    GATTATAATG TAACAGACTG GAGTACGTGT CAGCTGAGTG AGAAGGCAGT TTGTGGAAAC
    GGCATTAAAA CAAGGATGTT GGATTGTGTT CGAAGCGATG GCAAGTCGGT TGACCTGAAA
    TATTGTGAAG AGCTTGGCCT GGAGAAGACC TGGCAGATGA ACACATCCTG CATGGTAGAA
    TGTCCCGTCA ACTGCCAGCT TTCCGACTGG TCTCCTTGGT CAGAATGTTC TCAAACATGT
    GGCCTCACAG GGAAAATGAT CCGAAGACGC ACAGTGACCC AGCCCTTTCA AGGTGATGGA
    CGGCCATGCC CTGCCCTGAT GGAACAGTCC AAGCCTTGCC CAGTGAAGCC CTGTTATCAG
    TGGCAATATG GCCAGTGGTC TCTATGCCAA GTGCAGGATG CCCAGTGTGG CGAAGGGACG
    AGAACAAGGA ACATTTCTTG TGTAGTGAGT GACGGGTCAA CTGATGATTT CAACAAAGTG
    GTGGATGAAG AATTCTGTAC TGACATTGAA CCCATAATAG ATGGTAATAA AAAAATGGTT
    CTCGAGGAAA CCTGCACCAA GCCTTGCCCA GGTGACTGTT ATCTGAAGGA CTGGTCTTCC
    TGGAGCCTGT GTCAGCTAAC CTGTGTGAAT GGTGAAGATC TAGGCTTTGG TGGAATACAG
    GTCCGTTCCA GAGCTGTGAT TATACAAGAG CTAGAAAATC AGCATCTGTG CCCAGAGCAA
    ATGTTAGAAA CAAAATCATG TAATGATGGA CAGTGTTATG AGTATAAATG GATGGCCAGT
    GCTTGGAAGG GTTCTTCCCG AACAGTCTGG TGTCAAAGGT CAGATGGTAT AAATGTAACA
    GGGGGCTGCT TGGTGGTGCG CCAGCCTGAT GCTGACAGGT CTTGTAACCC ACCGTGTAGT
    CAACCCCACT CGTACTGTAG TGAGATGAGG ACATGCAGTT GTGAAGAAGG GTATACTGAA
    GTCATGTCTT CTAACGGTAC ACTTGAGCAA TGCACACTTA TCCCCGTGGT GGTGATACCC
    ACTGTGGAGG ACAAAAGAGG AGATGTGAAA ACCAGTCGGG CAGTACATCC AACCCAATCC
    TCCAGTAATC CAACAGGACG GGGAAGGACC TGGTTTCTAC AGCCATTTGG GCCAGATGGG
    AGACTAAAGA CCTGGGTTTA CGGTGTAGCA GCTGGGGCAT TTGTGTTACT CATCTTTATT
    GTCTCCATGA TTTATCTAGC TTGCAAAAAG CCAAAGAAAC CCCAAAGAAG GCAAAACAAC
    CGACTGAAAC CTTTAACCCT AGCCTACGAT GGAGATGCAG ACATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003431913.2
    CDS269..5236
    Translation

    Target ORF information:

    RefSeq Version XM_003431865.5
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris thrombospondin type 1 domain containing 7A (THSD7A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003431865.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGCTGC AAGCGAGGCG CTCGGCGTCC CAGAGCCCCG GCGCCCCGGG CCCGCGCCGG 
    GGTGTCCTGC AGCTGCTGCC GCTGCTCCTG CTGCTGCTGC TGCTGCTGCT GCTGCGCCCC
    GGAGCCTGCG GGGCTGCGGC TCCCGGCGGG GCTGAGGCAC CCACCCTCTA CCTCTGGAAG
    ACTGGTCCAT GGGGCCGGTG TATGGGAGAT GAATGTGGTC CAGGAGGCAT TCAGACCCGG
    GCCGTGTGGT GTGCTCATGT GGAAGGATGG ACCACATTAC ATACAAACTG TAAGCAGGCT
    GAGAGACCCA GCAACCAGCA AAACTGTTTC AAGGTGTGTG ACTGGCACAA GGAGTTGTAT
    GATTGGAGGC TGGGACCTTG GAACCAGTGT CAGCCTGTGA TATCCAAGAG CCTGGAGAAA
    CCCCCTGAGT GCATTAAGGG GGAGGAAGGC ATCCAGGTGA GAGAAATAAC GTGCATCCAG
    AAAGAGAAGG ACATTCCTGC AGAGGATATC ATTTGTGAGT ACTTTGAGCC CAAACCTCTC
    CTGGAACAGG CCTGCCTCAT CCCCTGCCAG CAAGATTGCA TCGTGTCTGA GTTTTCCGCG
    TGGTCAGAAT GTTCCAAGAC TTGTGGCAGT GGGCTCCAGC ACCGGACTCG GCATGTGGTG
    GCGCCTCCCC AGTTTGGAGG TTCTGGCTGC CCAAACTTGA CAGAGTTTCA GGTGTGCCAG
    TCCAGCCCCT GTGAAGATGA AGAGAGCATG TACAGCTTGC ACGTGGGTCC CTGGAGCTCA
    TGCTCGATGT CCCACTCAAG ACAAGTAAGA CAAGCAAGGA GACGTGGGAA GAATAAAGAA
    CGGGAGAAGG ACCGAGGAAA AGGAGTGAAG GATCCCGAAG CCCGTGAGCT TATTAAGAAA
    AAGAGAAACA GAAACAGACA GAATCGACAA GAGAACAAAT ATTGGGACAT CCAGATTGGG
    TATCAGACCA GAGAGGTTAT GTGTACTAAC AAGACGGGAA AAGCTGTTGA TTTGAGCTTT
    TGCCAGCAAG AGAAGCTGCC AATGACCTTC CAGTCCTGTG TGATCACCAA AGAGTGCCAG
    GTGTCAGAGT GGTCAGAATG GAGTCCCTGC TCAAAAACAT GCCATGACAT GACATCCCCC
    AGAGGCACTC GCGTCAGGAC GCGGATCATT AGGCAGTTTC CTATTGGCAG CGAGAAAGAG
    TGTCCAGAAC TTGAAGAAAA AGAACCCTGT GTGTCTCAAG GAGATGCAGT TGCCCCCTGT
    GCCACGTATG GCTGGAGAAC TACAGAGTGG GCGGAGTGCC GTGTGGACCC CTTGCTCAGT
    CAGCAGGACA AGAGGCGGGG CAACCAGACA GCCCTCTGTG GAGGAGGAGT CCAGACACGA
    GAGGTGTACT GCGTGCAGGC CAATGAAAAC CTCCTCTCAC ACTTAAACAC CCACAAGGAC
    AAAGAAGCCT CAAAACCAGT GGACTTGAAA TCATGCACTG GCCCTGTCCC TAATACTACA
    CAGCTGTGCC ACATTCCCTG TCCTATTGAA TGTGAGGTTT CACCTTGGTC AGCTTGGGGA
    CCTTGTACTT ATGAAAACTG TAATGACCAG CAAGGCAAAA AAGGTTTCAA ACTGAGGAAG
    CGACGCATTA CCAATGAGCC CACTGGAGGC TCTGGGGGCA CTGGGAACTG CCCTCACTTA
    CTGGAAGCCA TTCCCTGTGA AGAGCCATCC TGCTATGAAT GGAAAGCAGT GAGGCTTGGA
    GACTGTGAAC CAGACAATGG AAAAGAGTGT GGCCCAGGCA CTCAAGTTCA GGAGGTTGTA
    TGCATCAACA GTGATGGAGA AGAAGTTGAC AGACAGCTGT GCAGAGATGC CATCTTCCCC
    ATCCCCGTTG CCTGTTATGC CCCATGCCCA AAGGACTGTG TGCTCAGCAC GTGGTCTGCA
    TGGTCCTCCT GCTCACACAC CTGCTCGGGG AAAACCACAG AAGGGAAACA GATACGAGCA
    CGGTCCATTC TGGCCTATGC CGGTGACGAA GGTGGAGCTC ACTGTCCAAA TAGCAGTGCT
    TTGCAAGAGG TGCGCAGTTG TAACGAGCAT CCCTGCACTG TGTACCACTG GCAGACTGGT
    CCCTGGGGCC AGTGCATAGA GGACACTTCA GTATCATCCT TCAACACGAC GACTACTTGG
    AATGGAGAGG CGTCTTGCTC TGTTGGCATG CAGACAAGAA AAGTCATTTG TGTGCGGGTC
    AATGTGGGCC AAGTAGGACC CAAAAAGTGT CCTGAAAGCC TTAGACCTGA AACAGTGAGG
    CCCTGTCTGC TTCCTTGTAG AAAGGACTGT ATTGTGACAC CATATAGTGA CTGGACATCA
    TGCCCCAACT CATGTAAAGA AGGGGACTCC GGAGTCAGAA AGCAGTCTCG GCACCGGGTC
    ATCATTCAGC AGCCGGCCAA CGGGGGCAGG GACTGCACGG ACCCGCTCTA CGAAGAGAAA
    GTCTGTGAGG CCCCTCAAGT GTGCCAAAGC TACAGGTGGA AGACCCACAA ATGGCGCAGG
    TGCCAGTTGG TCCCTTGGAG CGTGCGACAG GACAGCCCTG GAGCACAGGA AGGCTGCGGA
    CCCGGACGCC AGGCAAGAGC TATTACTTGT CGAAAGCAAG ATGGAGGACA GGCTGGCATC
    CATGAATGCC TCTGGTATGC AGGCCCTGTG CCAGCCCTCA CCCAGGCCTG CCAGATCCCA
    TGCCAAGATG ACTGTCAGTT TACCAGCTGG TCCAAGTTTT CTTCCTGCAA CGGAGACTGT
    GGTGCAGTTA GGACCAGAAA ACGCACTATT GTTGGAAAAA GTAAAAAGAA GGAAAAATGT
    AAAAATTCTC ATTTGTACCC CCTGATTGAG ACTCAGTACT GTCCTTGTGA CAAGTATAAT
    GCACAGCCTG TGGGGAACTG GTCAGACTGC ATTTTACCAG AGGGGAAAGT GGAAGTGTTG
    CTTGGAGTGA AAACACAAGG AGATACCAAG GAATGTGGTC AAGGATATCG TTACCAAGCA
    ATGGCATGCT ACGACCAAAA TGGCAGGCTC GTGGAAACAT CCAGATGTAA CAGTCATGGT
    TACATTGAAG AGGCCTGCAT CATCCCTTGT CCTTCTGATT GCAAGCTCAG TGAGTGGTCC
    AACTGGTCTC GCTGCAGTAA GTCCTGTGGA AGTGGTGTGA AGGTTCGGTC TAAATGGTTG
    CGTGAGAAAC CATATAATGG AGGGAGGCCT TGCCCCAAGC TGGACCATGT CAACCAGGCA
    CAGGTGTATG AGGTTGTCCC TTGCCACAGC GACTGCAACC AGTACATATG GGTCACAGAG
    CCATGGAGCA TCTGCAAAGT GACCTTTGTC AACATGCGAG ATAACTGTGG AGAGGGTGTG
    CAAACCCGAA AAGTGAGATG CATGCAGAAC ACAGCAGATG GCCCTTCTGA CCATGTAGAA
    GATTACCTCT GCGATCCAGA AGAGATGCCC CTGGGCTCTA GAGAGTGCAA ATTACCATGC
    CCTGAGGATT GTGTGATATC TGAATGGAGT CCATGGACCC GCTGCACTTT GCCTTGCAGT
    CAAAGCAGTT TCCGGCAAAG GTCAGCTGAT CCCATCAGAC AACCTGCAGA TGAAGGAAGA
    GCCTGCCCTA ATGCTGTTGA GAAGGAACCC TGTAACCTAA ACAAAAACTG CTTCCACTAT
    GATTATAATG TAACAGACTG GAGTACGTGT CAGCTGAGTG AGAAGGCAGT TTGTGGAAAC
    GGCATTAAAA CAAGGATGTT GGATTGTGTT CGAAGCGATG GCAAGTCGGT TGACCTGAAA
    TATTGTGAAG AGCTTGGCCT GGAGAAGACC TGGCAGATGA ACACATCCTG CATGGTAGAA
    TGTCCCGTCA ACTGCCAGCT TTCCGACTGG TCTCCTTGGT CAGAATGTTC TCAAACATGT
    GGCCTCACAG GGAAAATGAT CCGAAGACGC ACAGTGACCC AGCCCTTTCA AGGTGATGGA
    CGGCCATGCC CTGCCCTGAT GGAACAGTCC AAGCCTTGCC CAGTGAAGCC CTGTTATCAG
    TGGCAATATG GCCAGTGGTC TCTATGCCAA GTGCAGGATG CCCAGTGTGG CGAAGGGACG
    AGAACAAGGA ACATTTCTTG TGTAGTGAGT GACGGGTCAA CTGATGATTT CAACAAAGTG
    GTGGATGAAG AATTCTGTAC TGACATTGAA CCCATAATAG ATGGTAATAA AAAAATGGTT
    CTCGAGGAAA CCTGCACCAA GCCTTGCCCA GGTGACTGTT ATCTGAAGGA CTGGTCTTCC
    TGGAGCCTGT GTCAGCTAAC CTGTGTGAAT GGTGAAGATC TAGGCTTTGG TGGAATACAG
    GTCCGTTCCA GAGCTGTGAT TATACAAGAG CTAGAAAATC AGCATCTGTG CCCAGAGCAA
    ATGTTAGAAA CAAAATCATG TAATGATGGA CAGTGTTATG AGTATAAATG GATGGCCAGT
    GCTTGGAAGG GTTCTTCCCG AACAGTCTGG TGTCAAAGGT CAGATGGTAT AAATGTAACA
    GGGGGCTGCT TGGTGGTGCG CCAGCCTGAT GCTGACAGGT CTTGTAACCC ACCGTGTAGT
    CAACCCCACT CGTACTGTAG TGAGATGAGG ACATGCAGTT GTGAAGAAGG GTATACTGAA
    GTCATGTCTT CTAACGGTAC ACTTGAGCAA TGCACACTTA TCCCCGTGGT GGTGATACCC
    ACTGTGGAGG ACAAAAGAGG AGATGTGAAA ACCAGTCGGG CAGTACATCC AACCCAATCC
    TCCAGTAATC CAACAGGACG GGGAAGGACC TGGTTTCTAC AGCCATTTGG GCCAGATGGG
    AGACTAAAGA CCTGGGTTTA CGGTGTAGCA GCTGGGGCAT TTGTGTTACT CATCTTTATT
    GTCTCCATGA TTTATCTAGC TTGCAAAAAG CCAAAGAAAC CCCAAAGAAG GCAAAACAAC
    CGACTGAAAC CTTTAACCCT AGCCTACGAT GGAGATGCAG ACATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe16747
    Clone ID Related Accession (Same CDS sequence) XM_003431865.5 , XM_003431865.4
    Accession Version XM_003431865.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4968bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product thrombospondin type-1 domain-containing protein 7A isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726084.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003431865.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGCTGC AAGCGAGGCG CTCGGCGTCC CAGAGCCCCG GCGCCCCGGG CCCGCGCCGG 
    GGTGTCCTGC AGCTGCTGCC GCTGCTCCTG CTGCTGCTGC TGCTGCTGCT GCTGCGCCCC
    GGAGCCTGCG GGGCTGCGGC TCCCGGCGGG GCTGAGGCAC CCACCCTCTA CCTCTGGAAG
    ACTGGTCCAT GGGGCCGGTG TATGGGAGAT GAATGTGGTC CAGGAGGCAT TCAGACCCGG
    GCCGTGTGGT GTGCTCATGT GGAAGGATGG ACCACATTAC ATACAAACTG TAAGCAGGCT
    GAGAGACCCA GCAACCAGCA AAACTGTTTC AAGGTGTGTG ACTGGCACAA GGAGTTGTAT
    GATTGGAGGC TGGGACCTTG GAACCAGTGT CAGCCTGTGA TATCCAAGAG CCTGGAGAAA
    CCCCCTGAGT GCATTAAGGG GGAGGAAGGC ATCCAGGTGA GAGAAATAAC GTGCATCCAG
    AAAGAGAAGG ACATTCCTGC AGAGGATATC ATTTGTGAGT ACTTTGAGCC CAAACCTCTC
    CTGGAACAGG CCTGCCTCAT CCCCTGCCAG CAAGATTGCA TCGTGTCTGA GTTTTCCGCG
    TGGTCAGAAT GTTCCAAGAC TTGTGGCAGT GGGCTCCAGC ACCGGACTCG GCATGTGGTG
    GCGCCTCCCC AGTTTGGAGG TTCTGGCTGC CCAAACTTGA CAGAGTTTCA GGTGTGCCAG
    TCCAGCCCCT GTGAAGATGA AGAGAGCATG TACAGCTTGC ACGTGGGTCC CTGGAGCTCA
    TGCTCGATGT CCCACTCAAG ACAAGTAAGA CAAGCAAGGA GACGTGGGAA GAATAAAGAA
    CGGGAGAAGG ACCGAGGAAA AGGAGTGAAG GATCCCGAAG CCCGTGAGCT TATTAAGAAA
    AAGAGAAACA GAAACAGACA GAATCGACAA GAGAACAAAT ATTGGGACAT CCAGATTGGG
    TATCAGACCA GAGAGGTTAT GTGTACTAAC AAGACGGGAA AAGCTGTTGA TTTGAGCTTT
    TGCCAGCAAG AGAAGCTGCC AATGACCTTC CAGTCCTGTG TGATCACCAA AGAGTGCCAG
    GTGTCAGAGT GGTCAGAATG GAGTCCCTGC TCAAAAACAT GCCATGACAT GACATCCCCC
    AGAGGCACTC GCGTCAGGAC GCGGATCATT AGGCAGTTTC CTATTGGCAG CGAGAAAGAG
    TGTCCAGAAC TTGAAGAAAA AGAACCCTGT GTGTCTCAAG GAGATGCAGT TGCCCCCTGT
    GCCACGTATG GCTGGAGAAC TACAGAGTGG GCGGAGTGCC GTGTGGACCC CTTGCTCAGT
    CAGCAGGACA AGAGGCGGGG CAACCAGACA GCCCTCTGTG GAGGAGGAGT CCAGACACGA
    GAGGTGTACT GCGTGCAGGC CAATGAAAAC CTCCTCTCAC ACTTAAACAC CCACAAGGAC
    AAAGAAGCCT CAAAACCAGT GGACTTGAAA TCATGCACTG GCCCTGTCCC TAATACTACA
    CAGCTGTGCC ACATTCCCTG TCCTATTGAA TGTGAGGTTT CACCTTGGTC AGCTTGGGGA
    CCTTGTACTT ATGAAAACTG TAATGACCAG CAAGGCAAAA AAGGTTTCAA ACTGAGGAAG
    CGACGCATTA CCAATGAGCC CACTGGAGGC TCTGGGGGCA CTGGGAACTG CCCTCACTTA
    CTGGAAGCCA TTCCCTGTGA AGAGCCATCC TGCTATGAAT GGAAAGCAGT GAGGCTTGGA
    GACTGTGAAC CAGACAATGG AAAAGAGTGT GGCCCAGGCA CTCAAGTTCA GGAGGTTGTA
    TGCATCAACA GTGATGGAGA AGAAGTTGAC AGACAGCTGT GCAGAGATGC CATCTTCCCC
    ATCCCCGTTG CCTGTTATGC CCCATGCCCA AAGGACTGTG TGCTCAGCAC GTGGTCTGCA
    TGGTCCTCCT GCTCACACAC CTGCTCGGGG AAAACCACAG AAGGGAAACA GATACGAGCA
    CGGTCCATTC TGGCCTATGC CGGTGACGAA GGTGGAGCTC ACTGTCCAAA TAGCAGTGCT
    TTGCAAGAGG TGCGCAGTTG TAACGAGCAT CCCTGCACTG TGTACCACTG GCAGACTGGT
    CCCTGGGGCC AGTGCATAGA GGACACTTCA GTATCATCCT TCAACACGAC GACTACTTGG
    AATGGAGAGG CGTCTTGCTC TGTTGGCATG CAGACAAGAA AAGTCATTTG TGTGCGGGTC
    AATGTGGGCC AAGTAGGACC CAAAAAGTGT CCTGAAAGCC TTAGACCTGA AACAGTGAGG
    CCCTGTCTGC TTCCTTGTAG AAAGGACTGT ATTGTGACAC CATATAGTGA CTGGACATCA
    TGCCCCAACT CATGTAAAGA AGGGGACTCC GGAGTCAGAA AGCAGTCTCG GCACCGGGTC
    ATCATTCAGC AGCCGGCCAA CGGGGGCAGG GACTGCACGG ACCCGCTCTA CGAAGAGAAA
    GTCTGTGAGG CCCCTCAAGT GTGCCAAAGC TACAGGTGGA AGACCCACAA ATGGCGCAGG
    TGCCAGTTGG TCCCTTGGAG CGTGCGACAG GACAGCCCTG GAGCACAGGA AGGCTGCGGA
    CCCGGACGCC AGGCAAGAGC TATTACTTGT CGAAAGCAAG ATGGAGGACA GGCTGGCATC
    CATGAATGCC TCTGGTATGC AGGCCCTGTG CCAGCCCTCA CCCAGGCCTG CCAGATCCCA
    TGCCAAGATG ACTGTCAGTT TACCAGCTGG TCCAAGTTTT CTTCCTGCAA CGGAGACTGT
    GGTGCAGTTA GGACCAGAAA ACGCACTATT GTTGGAAAAA GTAAAAAGAA GGAAAAATGT
    AAAAATTCTC ATTTGTACCC CCTGATTGAG ACTCAGTACT GTCCTTGTGA CAAGTATAAT
    GCACAGCCTG TGGGGAACTG GTCAGACTGC ATTTTACCAG AGGGGAAAGT GGAAGTGTTG
    CTTGGAGTGA AAACACAAGG AGATACCAAG GAATGTGGTC AAGGATATCG TTACCAAGCA
    ATGGCATGCT ACGACCAAAA TGGCAGGCTC GTGGAAACAT CCAGATGTAA CAGTCATGGT
    TACATTGAAG AGGCCTGCAT CATCCCTTGT CCTTCTGATT GCAAGCTCAG TGAGTGGTCC
    AACTGGTCTC GCTGCAGTAA GTCCTGTGGA AGTGGTGTGA AGGTTCGGTC TAAATGGTTG
    CGTGAGAAAC CATATAATGG AGGGAGGCCT TGCCCCAAGC TGGACCATGT CAACCAGGCA
    CAGGTGTATG AGGTTGTCCC TTGCCACAGC GACTGCAACC AGTACATATG GGTCACAGAG
    CCATGGAGCA TCTGCAAAGT GACCTTTGTC AACATGCGAG ATAACTGTGG AGAGGGTGTG
    CAAACCCGAA AAGTGAGATG CATGCAGAAC ACAGCAGATG GCCCTTCTGA CCATGTAGAA
    GATTACCTCT GCGATCCAGA AGAGATGCCC CTGGGCTCTA GAGAGTGCAA ATTACCATGC
    CCTGAGGATT GTGTGATATC TGAATGGAGT CCATGGACCC GCTGCACTTT GCCTTGCAGT
    CAAAGCAGTT TCCGGCAAAG GTCAGCTGAT CCCATCAGAC AACCTGCAGA TGAAGGAAGA
    GCCTGCCCTA ATGCTGTTGA GAAGGAACCC TGTAACCTAA ACAAAAACTG CTTCCACTAT
    GATTATAATG TAACAGACTG GAGTACGTGT CAGCTGAGTG AGAAGGCAGT TTGTGGAAAC
    GGCATTAAAA CAAGGATGTT GGATTGTGTT CGAAGCGATG GCAAGTCGGT TGACCTGAAA
    TATTGTGAAG AGCTTGGCCT GGAGAAGACC TGGCAGATGA ACACATCCTG CATGGTAGAA
    TGTCCCGTCA ACTGCCAGCT TTCCGACTGG TCTCCTTGGT CAGAATGTTC TCAAACATGT
    GGCCTCACAG GGAAAATGAT CCGAAGACGC ACAGTGACCC AGCCCTTTCA AGGTGATGGA
    CGGCCATGCC CTGCCCTGAT GGAACAGTCC AAGCCTTGCC CAGTGAAGCC CTGTTATCAG
    TGGCAATATG GCCAGTGGTC TCTATGCCAA GTGCAGGATG CCCAGTGTGG CGAAGGGACG
    AGAACAAGGA ACATTTCTTG TGTAGTGAGT GACGGGTCAA CTGATGATTT CAACAAAGTG
    GTGGATGAAG AATTCTGTAC TGACATTGAA CCCATAATAG ATGGTAATAA AAAAATGGTT
    CTCGAGGAAA CCTGCACCAA GCCTTGCCCA GGTGACTGTT ATCTGAAGGA CTGGTCTTCC
    TGGAGCCTGT GTCAGCTAAC CTGTGTGAAT GGTGAAGATC TAGGCTTTGG TGGAATACAG
    GTCCGTTCCA GAGCTGTGAT TATACAAGAG CTAGAAAATC AGCATCTGTG CCCAGAGCAA
    ATGTTAGAAA CAAAATCATG TAATGATGGA CAGTGTTATG AGTATAAATG GATGGCCAGT
    GCTTGGAAGG GTTCTTCCCG AACAGTCTGG TGTCAAAGGT CAGATGGTAT AAATGTAACA
    GGGGGCTGCT TGGTGGTGCG CCAGCCTGAT GCTGACAGGT CTTGTAACCC ACCGTGTAGT
    CAACCCCACT CGTACTGTAG TGAGATGAGG ACATGCAGTT GTGAAGAAGG GTATACTGAA
    GTCATGTCTT CTAACGGTAC ACTTGAGCAA TGCACACTTA TCCCCGTGGT GGTGATACCC
    ACTGTGGAGG ACAAAAGAGG AGATGTGAAA ACCAGTCGGG CAGTACATCC AACCCAATCC
    TCCAGTAATC CAACAGGACG GGGAAGGACC TGGTTTCTAC AGCCATTTGG GCCAGATGGG
    AGACTAAAGA CCTGGGTTTA CGGTGTAGCA GCTGGGGCAT TTGTGTTACT CATCTTTATT
    GTCTCCATGA TTTATCTAGC TTGCAAAAAG CCAAAGAAAC CCCAAAGAAG GCAAAACAAC
    CGACTGAAAC CTTTAACCCT AGCCTACGAT GGAGATGCAG ACATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003431913.2
    CDS343..5310
    Translation

    Target ORF information:

    RefSeq Version XM_003431865.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris thrombospondin, type I, domain containing 7A (THSD7A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003431865.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGCTGC AAGCGAGGCG CTCGGCGTCC CAGAGCCCCG GCGCCCCGGG CCCGCGCCGG 
    GGTGTCCTGC AGCTGCTGCC GCTGCTCCTG CTGCTGCTGC TGCTGCTGCT GCTGCGCCCC
    GGAGCCTGCG GGGCTGCGGC TCCCGGCGGG GCTGAGGCAC CCACCCTCTA CCTCTGGAAG
    ACTGGTCCAT GGGGCCGGTG TATGGGAGAT GAATGTGGTC CAGGAGGCAT TCAGACCCGG
    GCCGTGTGGT GTGCTCATGT GGAAGGATGG ACCACATTAC ATACAAACTG TAAGCAGGCT
    GAGAGACCCA GCAACCAGCA AAACTGTTTC AAGGTGTGTG ACTGGCACAA GGAGTTGTAT
    GATTGGAGGC TGGGACCTTG GAACCAGTGT CAGCCTGTGA TATCCAAGAG CCTGGAGAAA
    CCCCCTGAGT GCATTAAGGG GGAGGAAGGC ATCCAGGTGA GAGAAATAAC GTGCATCCAG
    AAAGAGAAGG ACATTCCTGC AGAGGATATC ATTTGTGAGT ACTTTGAGCC CAAACCTCTC
    CTGGAACAGG CCTGCCTCAT CCCCTGCCAG CAAGATTGCA TCGTGTCTGA GTTTTCCGCG
    TGGTCAGAAT GTTCCAAGAC TTGTGGCAGT GGGCTCCAGC ACCGGACTCG GCATGTGGTG
    GCGCCTCCCC AGTTTGGAGG TTCTGGCTGC CCAAACTTGA CAGAGTTTCA GGTGTGCCAG
    TCCAGCCCCT GTGAAGATGA AGAGAGCATG TACAGCTTGC ACGTGGGTCC CTGGAGCTCA
    TGCTCGATGT CCCACTCAAG ACAAGTAAGA CAAGCAAGGA GACGTGGGAA GAATAAAGAA
    CGGGAGAAGG ACCGAGGAAA AGGAGTGAAG GATCCCGAAG CCCGTGAGCT TATTAAGAAA
    AAGAGAAACA GAAACAGACA GAATCGACAA GAGAACAAAT ATTGGGACAT CCAGATTGGG
    TATCAGACCA GAGAGGTTAT GTGTACTAAC AAGACGGGAA AAGCTGTTGA TTTGAGCTTT
    TGCCAGCAAG AGAAGCTGCC AATGACCTTC CAGTCCTGTG TGATCACCAA AGAGTGCCAG
    GTGTCAGAGT GGTCAGAATG GAGTCCCTGC TCAAAAACAT GCCATGACAT GACATCCCCC
    AGAGGCACTC GCGTCAGGAC GCGGATCATT AGGCAGTTTC CTATTGGCAG CGAGAAAGAG
    TGTCCAGAAC TTGAAGAAAA AGAACCCTGT GTGTCTCAAG GAGATGCAGT TGCCCCCTGT
    GCCACGTATG GCTGGAGAAC TACAGAGTGG GCGGAGTGCC GTGTGGACCC CTTGCTCAGT
    CAGCAGGACA AGAGGCGGGG CAACCAGACA GCCCTCTGTG GAGGAGGAGT CCAGACACGA
    GAGGTGTACT GCGTGCAGGC CAATGAAAAC CTCCTCTCAC ACTTAAACAC CCACAAGGAC
    AAAGAAGCCT CAAAACCAGT GGACTTGAAA TCATGCACTG GCCCTGTCCC TAATACTACA
    CAGCTGTGCC ACATTCCCTG TCCTATTGAA TGTGAGGTTT CACCTTGGTC AGCTTGGGGA
    CCTTGTACTT ATGAAAACTG TAATGACCAG CAAGGCAAAA AAGGTTTCAA ACTGAGGAAG
    CGACGCATTA CCAATGAGCC CACTGGAGGC TCTGGGGGCA CTGGGAACTG CCCTCACTTA
    CTGGAAGCCA TTCCCTGTGA AGAGCCATCC TGCTATGAAT GGAAAGCAGT GAGGCTTGGA
    GACTGTGAAC CAGACAATGG AAAAGAGTGT GGCCCAGGCA CTCAAGTTCA GGAGGTTGTA
    TGCATCAACA GTGATGGAGA AGAAGTTGAC AGACAGCTGT GCAGAGATGC CATCTTCCCC
    ATCCCCGTTG CCTGTTATGC CCCATGCCCA AAGGACTGTG TGCTCAGCAC GTGGTCTGCA
    TGGTCCTCCT GCTCACACAC CTGCTCGGGG AAAACCACAG AAGGGAAACA GATACGAGCA
    CGGTCCATTC TGGCCTATGC CGGTGACGAA GGTGGAGCTC ACTGTCCAAA TAGCAGTGCT
    TTGCAAGAGG TGCGCAGTTG TAACGAGCAT CCCTGCACTG TGTACCACTG GCAGACTGGT
    CCCTGGGGCC AGTGCATAGA GGACACTTCA GTATCATCCT TCAACACGAC GACTACTTGG
    AATGGAGAGG CGTCTTGCTC TGTTGGCATG CAGACAAGAA AAGTCATTTG TGTGCGGGTC
    AATGTGGGCC AAGTAGGACC CAAAAAGTGT CCTGAAAGCC TTAGACCTGA AACAGTGAGG
    CCCTGTCTGC TTCCTTGTAG AAAGGACTGT ATTGTGACAC CATATAGTGA CTGGACATCA
    TGCCCCAACT CATGTAAAGA AGGGGACTCC GGAGTCAGAA AGCAGTCTCG GCACCGGGTC
    ATCATTCAGC AGCCGGCCAA CGGGGGCAGG GACTGCACGG ACCCGCTCTA CGAAGAGAAA
    GTCTGTGAGG CCCCTCAAGT GTGCCAAAGC TACAGGTGGA AGACCCACAA ATGGCGCAGG
    TGCCAGTTGG TCCCTTGGAG CGTGCGACAG GACAGCCCTG GAGCACAGGA AGGCTGCGGA
    CCCGGACGCC AGGCAAGAGC TATTACTTGT CGAAAGCAAG ATGGAGGACA GGCTGGCATC
    CATGAATGCC TCTGGTATGC AGGCCCTGTG CCAGCCCTCA CCCAGGCCTG CCAGATCCCA
    TGCCAAGATG ACTGTCAGTT TACCAGCTGG TCCAAGTTTT CTTCCTGCAA CGGAGACTGT
    GGTGCAGTTA GGACCAGAAA ACGCACTATT GTTGGAAAAA GTAAAAAGAA GGAAAAATGT
    AAAAATTCTC ATTTGTACCC CCTGATTGAG ACTCAGTACT GTCCTTGTGA CAAGTATAAT
    GCACAGCCTG TGGGGAACTG GTCAGACTGC ATTTTACCAG AGGGGAAAGT GGAAGTGTTG
    CTTGGAGTGA AAACACAAGG AGATACCAAG GAATGTGGTC AAGGATATCG TTACCAAGCA
    ATGGCATGCT ACGACCAAAA TGGCAGGCTC GTGGAAACAT CCAGATGTAA CAGTCATGGT
    TACATTGAAG AGGCCTGCAT CATCCCTTGT CCTTCTGATT GCAAGCTCAG TGAGTGGTCC
    AACTGGTCTC GCTGCAGTAA GTCCTGTGGA AGTGGTGTGA AGGTTCGGTC TAAATGGTTG
    CGTGAGAAAC CATATAATGG AGGGAGGCCT TGCCCCAAGC TGGACCATGT CAACCAGGCA
    CAGGTGTATG AGGTTGTCCC TTGCCACAGC GACTGCAACC AGTACATATG GGTCACAGAG
    CCATGGAGCA TCTGCAAAGT GACCTTTGTC AACATGCGAG ATAACTGTGG AGAGGGTGTG
    CAAACCCGAA AAGTGAGATG CATGCAGAAC ACAGCAGATG GCCCTTCTGA CCATGTAGAA
    GATTACCTCT GCGATCCAGA AGAGATGCCC CTGGGCTCTA GAGAGTGCAA ATTACCATGC
    CCTGAGGATT GTGTGATATC TGAATGGAGT CCATGGACCC GCTGCACTTT GCCTTGCAGT
    CAAAGCAGTT TCCGGCAAAG GTCAGCTGAT CCCATCAGAC AACCTGCAGA TGAAGGAAGA
    GCCTGCCCTA ATGCTGTTGA GAAGGAACCC TGTAACCTAA ACAAAAACTG CTTCCACTAT
    GATTATAATG TAACAGACTG GAGTACGTGT CAGCTGAGTG AGAAGGCAGT TTGTGGAAAC
    GGCATTAAAA CAAGGATGTT GGATTGTGTT CGAAGCGATG GCAAGTCGGT TGACCTGAAA
    TATTGTGAAG AGCTTGGCCT GGAGAAGACC TGGCAGATGA ACACATCCTG CATGGTAGAA
    TGTCCCGTCA ACTGCCAGCT TTCCGACTGG TCTCCTTGGT CAGAATGTTC TCAAACATGT
    GGCCTCACAG GGAAAATGAT CCGAAGACGC ACAGTGACCC AGCCCTTTCA AGGTGATGGA
    CGGCCATGCC CTGCCCTGAT GGAACAGTCC AAGCCTTGCC CAGTGAAGCC CTGTTATCAG
    TGGCAATATG GCCAGTGGTC TCTATGCCAA GTGCAGGATG CCCAGTGTGG CGAAGGGACG
    AGAACAAGGA ACATTTCTTG TGTAGTGAGT GACGGGTCAA CTGATGATTT CAACAAAGTG
    GTGGATGAAG AATTCTGTAC TGACATTGAA CCCATAATAG ATGGTAATAA AAAAATGGTT
    CTCGAGGAAA CCTGCACCAA GCCTTGCCCA GGTGACTGTT ATCTGAAGGA CTGGTCTTCC
    TGGAGCCTGT GTCAGCTAAC CTGTGTGAAT GGTGAAGATC TAGGCTTTGG TGGAATACAG
    GTCCGTTCCA GAGCTGTGAT TATACAAGAG CTAGAAAATC AGCATCTGTG CCCAGAGCAA
    ATGTTAGAAA CAAAATCATG TAATGATGGA CAGTGTTATG AGTATAAATG GATGGCCAGT
    GCTTGGAAGG GTTCTTCCCG AACAGTCTGG TGTCAAAGGT CAGATGGTAT AAATGTAACA
    GGGGGCTGCT TGGTGGTGCG CCAGCCTGAT GCTGACAGGT CTTGTAACCC ACCGTGTAGT
    CAACCCCACT CGTACTGTAG TGAGATGAGG ACATGCAGTT GTGAAGAAGG GTATACTGAA
    GTCATGTCTT CTAACGGTAC ACTTGAGCAA TGCACACTTA TCCCCGTGGT GGTGATACCC
    ACTGTGGAGG ACAAAAGAGG AGATGTGAAA ACCAGTCGGG CAGTACATCC AACCCAATCC
    TCCAGTAATC CAACAGGACG GGGAAGGACC TGGTTTCTAC AGCCATTTGG GCCAGATGGG
    AGACTAAAGA CCTGGGTTTA CGGTGTAGCA GCTGGGGCAT TTGTGTTACT CATCTTTATT
    GTCTCCATGA TTTATCTAGC TTGCAAAAAG CCAAAGAAAC CCCAAAGAAG GCAAAACAAC
    CGACTGAAAC CTTTAACCCT AGCCTACGAT GGAGATGCAG ACATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe16748
    Clone ID Related Accession (Same CDS sequence) XM_005628561.2 , XM_005628561.3
    Accession Version XM_005628561.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4497bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product thrombospondin type-1 domain-containing protein 7A isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726084.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005628561.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGGGCTGC AAGCGAGGCG CTCGGCGTCC CAGAGCCCCG GCGCCCCGGG CCCGCGCCGG 
    GGTGTCCTGC AGCTGCTGCC GCTGCTCCTG CTGCTGCTGC TGCTGCTGCT GCTGCGCCCC
    GGAGCCTGCG GGGCTGCGGC TCCCGGCGGG GCTGAGGCAC CCACCCTCTA CCTCTGGAAG
    ACTGGTCCAT GGGGCCGGTG TATGGGAGAT GAATGTGGTC CAGGAGGCAT TCAGACCCGG
    GCCGTGTGGT GTGCTCATGT GGAAGGATGG ACCACATTAC ATACAAACTG TAAGCAGGCT
    GAGAGACCCA GCAACCAGCA AAACTGTTTC AAGGTGTGTG ACTGGCACAA GGAGTTGTAT
    GATTGGAGGC TGGGACCTTG GAACCAGTGT CAGCCTGTGA TATCCAAGAG CCTGGAGAAA
    CCCCCTGAGT GCATTAAGGG GGAGGAAGGC ATCCAGGTGA GAGAAATAAC GTGCATCCAG
    AAAGAGAAGG ACATTCCTGC AGAGGATATC ATTTGTGAGT ACTTTGAGCC CAAACCTCTC
    CTGGAACAGG CCTGCCTCAT CCCCTGCCAG CAAGATTGCA TCGTGTCTGA GTTTTCCGCG
    TGGTCAGAAT GTTCCAAGAC TTGTGGCAGT GGGCTCCAGC ACCGGACTCG GCATGTGGTG
    GCGCCTCCCC AGTTTGGAGG TTCTGGCTGC CCAAACTTGA CAGAGTTTCA GGTGTGCCAG
    TCCAGCCCCT GTGAAGATGA AGAGAGCATG TACAGCTTGC ACGTGGGTCC CTGGAGCTCA
    TGCTCGATGT CCCACTCAAG ACAAGTAAGA CAAGCAAGGA GACGTGGGAA GAATAAAGAA
    CGGGAGAAGG ACCGAGGAAA AGGAGTGAAG GATCCCGAAG CCCGTGAGCT TATTAAGAAA
    AAGAGAAACA GAAACAGACA GAATCGACAA GAGAACAAAT ATTGGGACAT CCAGATTGGG
    TATCAGACCA GAGAGGTTAT GTGTACTAAC AAGACGGGAA AAGCTGTTGA TTTGAGCTTT
    TGCCAGCAAG AGAAGCTGCC AATGACCTTC CAGTCCTGTG TGATCACCAA AGAGTGCCAG
    GTGTCAGAGT GGTCAGAATG GAGTCCCTGC TCAAAAACAT GCCATGACAT GACATCCCCC
    AGAGGCACTC GCGTCAGGAC GCGGATCATT AGGCAGTTTC CTATTGGCAG CGAGAAAGAG
    TGTCCAGAAC TTGAAGAAAA AGAACCCTGT GTGTCTCAAG GAGATGCAGT TGCCCCCTGT
    GCCACGTATG GCTGGAGAAC TACAGAGTGG GCGGAGTGCC GTGTGGACCC CTTGCTCAGT
    CAGCAGGACA AGAGGCGGGG CAACCAGACA GCCCTCTGTG GAGGAGGAGT CCAGACACGA
    GAGGTGTACT GCGTGCAGGC CAATGAAAAC CTCCTCTCAC ACTTAAACAC CCACAAGGAC
    AAAGAAGAAA GTAAATATCA GAGTACGTCG AAGTCTAGTG CTGCACCTTT CCTATCCATC
    ACTTTTGAAA TAACAGCCTC AAAACCAGTG GACTTGAAAT CATGCACTGG CCCTGTCCCT
    AATACTACAC AGCTGTGCCA CATTCCCTGT CCTATTGAAT GTGAGGTTTC ACCTTGGTCA
    GCTTGGGGAC CTTGTACTTA TGAAAACTGT AATGACCAGC AAGGCAAAAA AGGTTTCAAA
    CTGAGGAAGC GACGCATTAC CAATGAGCCC ACTGGAGGCT CTGGGGGCAC TGGGAACTGC
    CCTCACTTAC TGGAAGCCAT TCCCTGTGAA GAGCCATCCT GCTATGAATG GAAAGCAGTG
    AGGCTTGGAG ACTGTGAACC AGACAATGGA AAAGAGTGTG GCCCAGGCAC TCAAGTTCAG
    GAGGTTGTAT GCATCAACAG TGATGGAGAA GAAGTTGACA GACAGCTGTG CAGAGATGCC
    ATCTTCCCCA TCCCCGTTGC CTGTTATGCC CCATGCCCAA AGGACTGTGT GCTCAGCACG
    TGGTCTGCAT GGTCCTCCTG CTCACACACC TGCTCGGGGA AAACCACAGA AGGGAAACAG
    ATACGAGCAC GGTCCATTCT GGCCTATGCC GGTGACGAAG GTGGAGCTCA CTGTCCAAAT
    AGCAGTGCTT TGCAAGAGGT GCGCAGTTGT AACGAGCATC CCTGCACTGT GTACCACTGG
    CAGACTGGTC CCTGGGGCCA GTGCATAGAG GACACTTCAG TATCATCCTT CAACACGACG
    ACTACTTGGA ATGGAGAGGC GTCTTGCTCT GTTGGCATGC AGACAAGAAA AGTCATTTGT
    GTGCGGGTCA ATGTGGGCCA AGTAGGACCC AAAAAGTGTC CTGAAAGCCT TAGACCTGAA
    ACAGTGAGGC CCTGTCTGCT TCCTTGTAGA AAGGACTGTA TTGTGACACC ATATAGTGAC
    TGGACATCAT GCCCCAACTC ATGTAAAGAA GGGGACTCCG GAGTCAGAAA GCAGTCTCGG
    CACCGGGTCA TCATTCAGCA GCCGGCCAAC GGGGGCAGGG ACTGCACGGA CCCGCTCTAC
    GAAGAGAAAG TCTGTGAGGC CCCTCAAGTG TGCCAAAGCT ACAGGTGGAA GACCCACAAA
    TGGCGCAGGT GCCAGTTGGT CCCTTGGAGC GTGCGACAGG ACAGCCCTGG AGCACAGGAA
    GGCTGCGGAC CCGGACGCCA GGCAAGAGCT ATTACTTGTC GAAAGCAAGA TGGAGGACAG
    GCTGGCATCC ATGAATGCCT CTGGTATGCA GGCCCTGTGC CAGCCCTCAC CCAGGCCTGC
    CAGATCCCAT GCCAAGATGA CTGTCAGTTT ACCAGCTGGT CCAAGTTTTC TTCCTGCAAC
    GGAGACTGTG GTGCAGTTAG GACCAGAAAA CGCACTATTG TTGGAAAAAG TAAAAAGAAG
    GAAAAATGTA AAAATTCTCA TTTGTACCCC CTGATTGAGA CTCAGTACTG TCCTTGTGAC
    AAGTATAATG CACAGCCTGT GGGGAACTGG TCAGACTGCA TTTTACCAGA GGGGAAAGTG
    GAAGTGTTGC TTGGAGTGAA AACACAAGGA GATACCAAGG AATGTGGTCA AGGATATCGT
    TACCAAGCAA TGGCATGCTA CGACCAAAAT GGCAGGCTCG TGGAAACATC CAGATGTAAC
    AGTCATGGTT ACATTGAAGA GGCCTGCATC ATCCCTTGTC CTTCTGATTG CAAGCTCAGT
    GAGTGGTCCA ACTGGTCTCG CTGCAGTAAG TCCTGTGGAA GTGGTGTGAA GGTTCGGTCT
    AAATGGTTGC GTGAGAAACC ATATAATGGA GGGAGGCCTT GCCCCAAGCT GGACCATGTC
    AACCAGGCAC AGGTGTATGA GGTTGTCCCT TGCCACAGCG ACTGCAACCA GTACATATGG
    GTCACAGAGC CATGGAGCAT CTGCAAAGTG ACCTTTGTCA ACATGCGAGA TAACTGTGGA
    GAGGGTGTGC AAACCCGAAA AGTGAGATGC ATGCAGAACA CAGCAGATGG CCCTTCTGAC
    CATGTAGAAG ATTACCTCTG CGATCCAGAA GAGATGCCCC TGGGCTCTAG AGAGTGCAAA
    TTACCATGCC CTGAGGATTG TGTGATATCT GAATGGAGTC CATGGACCCG CTGCACTTTG
    CCTTGCAGTC AAAGCAGTTT CCGGCAAAGG TCAGCTGATC CCATCAGACA ACCTGCAGAT
    GAAGGAAGAG CCTGCCCTAA TGCTGTTGAG AAGGAACCCT GTAACCTAAA CAAAAACTGC
    TTCCACTATG ATTATAATGT AACAGACTGG AGTACGTGTC AGCTGAGTGA GAAGGCAGTT
    TGTGGAAACG GCATTAAAAC AAGGATGTTG GATTGTGTTC GAAGCGATGG CAAGTCGGTT
    GACCTGAAAT ATTGTGAAGA GCTTGGCCTG GAGAAGACCT GGCAGATGAA CACATCCTGC
    ATGGTAGAAT GTCCCGTCAA CTGCCAGCTT TCCGACTGGT CTCCTTGGTC AGAATGTTCT
    CAAACATGTG GCCTCACAGG GAAAATGATC CGAAGACGCA CAGTGACCCA GCCCTTTCAA
    GGTGATGGAC GGCCATGCCC TGCCCTGATG GAACAGTCCA AGCCTTGCCC AGTGAAGCCC
    TGTTATCAGT GGCAATATGG CCAGTGGTCT CTATGCCAAG TGCAGGATGC CCAGTGTGGC
    GAAGGGACGA GAACAAGGAA CATTTCTTGT GTAGTGAGTG ACGGGTCAAC TGATGATTTC
    AACAAAGTGG TGGATGAAGA ATTCTGTACT GACATTGAAC CCATAATAGA TGGTAATAAA
    AAAATGGTTC TCGAGGAAAC CTGCACCAAG CCTTGCCCAG GTGACTGTTA TCTGAAGGAC
    TGGTCTTCCT GGAGCCTGTG TCAGCTAACC TGTGTGAATG GTGAAGATCT AGGCTTTGGT
    GGAATACAGG TCCGTTCCAG AGCTGTGATT ATACAAGAGC TAGAAAATCA GCATCTGTGC
    CCAGAGCAAA TGTTAGAAAC AAAATCATGT AATGATATGT ACCTTAAAGA AGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005628618.1
    CDS344..4840
    Translation

    Target ORF information:

    RefSeq Version XM_005628561.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris thrombospondin, type I, domain containing 7A (THSD7A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005628561.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGGGCTGC AAGCGAGGCG CTCGGCGTCC CAGAGCCCCG GCGCCCCGGG CCCGCGCCGG 
    GGTGTCCTGC AGCTGCTGCC GCTGCTCCTG CTGCTGCTGC TGCTGCTGCT GCTGCGCCCC
    GGAGCCTGCG GGGCTGCGGC TCCCGGCGGG GCTGAGGCAC CCACCCTCTA CCTCTGGAAG
    ACTGGTCCAT GGGGCCGGTG TATGGGAGAT GAATGTGGTC CAGGAGGCAT TCAGACCCGG
    GCCGTGTGGT GTGCTCATGT GGAAGGATGG ACCACATTAC ATACAAACTG TAAGCAGGCT
    GAGAGACCCA GCAACCAGCA AAACTGTTTC AAGGTGTGTG ACTGGCACAA GGAGTTGTAT
    GATTGGAGGC TGGGACCTTG GAACCAGTGT CAGCCTGTGA TATCCAAGAG CCTGGAGAAA
    CCCCCTGAGT GCATTAAGGG GGAGGAAGGC ATCCAGGTGA GAGAAATAAC GTGCATCCAG
    AAAGAGAAGG ACATTCCTGC AGAGGATATC ATTTGTGAGT ACTTTGAGCC CAAACCTCTC
    CTGGAACAGG CCTGCCTCAT CCCCTGCCAG CAAGATTGCA TCGTGTCTGA GTTTTCCGCG
    TGGTCAGAAT GTTCCAAGAC TTGTGGCAGT GGGCTCCAGC ACCGGACTCG GCATGTGGTG
    GCGCCTCCCC AGTTTGGAGG TTCTGGCTGC CCAAACTTGA CAGAGTTTCA GGTGTGCCAG
    TCCAGCCCCT GTGAAGATGA AGAGAGCATG TACAGCTTGC ACGTGGGTCC CTGGAGCTCA
    TGCTCGATGT CCCACTCAAG ACAAGTAAGA CAAGCAAGGA GACGTGGGAA GAATAAAGAA
    CGGGAGAAGG ACCGAGGAAA AGGAGTGAAG GATCCCGAAG CCCGTGAGCT TATTAAGAAA
    AAGAGAAACA GAAACAGACA GAATCGACAA GAGAACAAAT ATTGGGACAT CCAGATTGGG
    TATCAGACCA GAGAGGTTAT GTGTACTAAC AAGACGGGAA AAGCTGTTGA TTTGAGCTTT
    TGCCAGCAAG AGAAGCTGCC AATGACCTTC CAGTCCTGTG TGATCACCAA AGAGTGCCAG
    GTGTCAGAGT GGTCAGAATG GAGTCCCTGC TCAAAAACAT GCCATGACAT GACATCCCCC
    AGAGGCACTC GCGTCAGGAC GCGGATCATT AGGCAGTTTC CTATTGGCAG CGAGAAAGAG
    TGTCCAGAAC TTGAAGAAAA AGAACCCTGT GTGTCTCAAG GAGATGCAGT TGCCCCCTGT
    GCCACGTATG GCTGGAGAAC TACAGAGTGG GCGGAGTGCC GTGTGGACCC CTTGCTCAGT
    CAGCAGGACA AGAGGCGGGG CAACCAGACA GCCCTCTGTG GAGGAGGAGT CCAGACACGA
    GAGGTGTACT GCGTGCAGGC CAATGAAAAC CTCCTCTCAC ACTTAAACAC CCACAAGGAC
    AAAGAAGAAA GTAAATATCA GAGTACGTCG AAGTCTAGTG CTGCACCTTT CCTATCCATC
    ACTTTTGAAA TAACAGCCTC AAAACCAGTG GACTTGAAAT CATGCACTGG CCCTGTCCCT
    AATACTACAC AGCTGTGCCA CATTCCCTGT CCTATTGAAT GTGAGGTTTC ACCTTGGTCA
    GCTTGGGGAC CTTGTACTTA TGAAAACTGT AATGACCAGC AAGGCAAAAA AGGTTTCAAA
    CTGAGGAAGC GACGCATTAC CAATGAGCCC ACTGGAGGCT CTGGGGGCAC TGGGAACTGC
    CCTCACTTAC TGGAAGCCAT TCCCTGTGAA GAGCCATCCT GCTATGAATG GAAAGCAGTG
    AGGCTTGGAG ACTGTGAACC AGACAATGGA AAAGAGTGTG GCCCAGGCAC TCAAGTTCAG
    GAGGTTGTAT GCATCAACAG TGATGGAGAA GAAGTTGACA GACAGCTGTG CAGAGATGCC
    ATCTTCCCCA TCCCCGTTGC CTGTTATGCC CCATGCCCAA AGGACTGTGT GCTCAGCACG
    TGGTCTGCAT GGTCCTCCTG CTCACACACC TGCTCGGGGA AAACCACAGA AGGGAAACAG
    ATACGAGCAC GGTCCATTCT GGCCTATGCC GGTGACGAAG GTGGAGCTCA CTGTCCAAAT
    AGCAGTGCTT TGCAAGAGGT GCGCAGTTGT AACGAGCATC CCTGCACTGT GTACCACTGG
    CAGACTGGTC CCTGGGGCCA GTGCATAGAG GACACTTCAG TATCATCCTT CAACACGACG
    ACTACTTGGA ATGGAGAGGC GTCTTGCTCT GTTGGCATGC AGACAAGAAA AGTCATTTGT
    GTGCGGGTCA ATGTGGGCCA AGTAGGACCC AAAAAGTGTC CTGAAAGCCT TAGACCTGAA
    ACAGTGAGGC CCTGTCTGCT TCCTTGTAGA AAGGACTGTA TTGTGACACC ATATAGTGAC
    TGGACATCAT GCCCCAACTC ATGTAAAGAA GGGGACTCCG GAGTCAGAAA GCAGTCTCGG
    CACCGGGTCA TCATTCAGCA GCCGGCCAAC GGGGGCAGGG ACTGCACGGA CCCGCTCTAC
    GAAGAGAAAG TCTGTGAGGC CCCTCAAGTG TGCCAAAGCT ACAGGTGGAA GACCCACAAA
    TGGCGCAGGT GCCAGTTGGT CCCTTGGAGC GTGCGACAGG ACAGCCCTGG AGCACAGGAA
    GGCTGCGGAC CCGGACGCCA GGCAAGAGCT ATTACTTGTC GAAAGCAAGA TGGAGGACAG
    GCTGGCATCC ATGAATGCCT CTGGTATGCA GGCCCTGTGC CAGCCCTCAC CCAGGCCTGC
    CAGATCCCAT GCCAAGATGA CTGTCAGTTT ACCAGCTGGT CCAAGTTTTC TTCCTGCAAC
    GGAGACTGTG GTGCAGTTAG GACCAGAAAA CGCACTATTG TTGGAAAAAG TAAAAAGAAG
    GAAAAATGTA AAAATTCTCA TTTGTACCCC CTGATTGAGA CTCAGTACTG TCCTTGTGAC
    AAGTATAATG CACAGCCTGT GGGGAACTGG TCAGACTGCA TTTTACCAGA GGGGAAAGTG
    GAAGTGTTGC TTGGAGTGAA AACACAAGGA GATACCAAGG AATGTGGTCA AGGATATCGT
    TACCAAGCAA TGGCATGCTA CGACCAAAAT GGCAGGCTCG TGGAAACATC CAGATGTAAC
    AGTCATGGTT ACATTGAAGA GGCCTGCATC ATCCCTTGTC CTTCTGATTG CAAGCTCAGT
    GAGTGGTCCA ACTGGTCTCG CTGCAGTAAG TCCTGTGGAA GTGGTGTGAA GGTTCGGTCT
    AAATGGTTGC GTGAGAAACC ATATAATGGA GGGAGGCCTT GCCCCAAGCT GGACCATGTC
    AACCAGGCAC AGGTGTATGA GGTTGTCCCT TGCCACAGCG ACTGCAACCA GTACATATGG
    GTCACAGAGC CATGGAGCAT CTGCAAAGTG ACCTTTGTCA ACATGCGAGA TAACTGTGGA
    GAGGGTGTGC AAACCCGAAA AGTGAGATGC ATGCAGAACA CAGCAGATGG CCCTTCTGAC
    CATGTAGAAG ATTACCTCTG CGATCCAGAA GAGATGCCCC TGGGCTCTAG AGAGTGCAAA
    TTACCATGCC CTGAGGATTG TGTGATATCT GAATGGAGTC CATGGACCCG CTGCACTTTG
    CCTTGCAGTC AAAGCAGTTT CCGGCAAAGG TCAGCTGATC CCATCAGACA ACCTGCAGAT
    GAAGGAAGAG CCTGCCCTAA TGCTGTTGAG AAGGAACCCT GTAACCTAAA CAAAAACTGC
    TTCCACTATG ATTATAATGT AACAGACTGG AGTACGTGTC AGCTGAGTGA GAAGGCAGTT
    TGTGGAAACG GCATTAAAAC AAGGATGTTG GATTGTGTTC GAAGCGATGG CAAGTCGGTT
    GACCTGAAAT ATTGTGAAGA GCTTGGCCTG GAGAAGACCT GGCAGATGAA CACATCCTGC
    ATGGTAGAAT GTCCCGTCAA CTGCCAGCTT TCCGACTGGT CTCCTTGGTC AGAATGTTCT
    CAAACATGTG GCCTCACAGG GAAAATGATC CGAAGACGCA CAGTGACCCA GCCCTTTCAA
    GGTGATGGAC GGCCATGCCC TGCCCTGATG GAACAGTCCA AGCCTTGCCC AGTGAAGCCC
    TGTTATCAGT GGCAATATGG CCAGTGGTCT CTATGCCAAG TGCAGGATGC CCAGTGTGGC
    GAAGGGACGA GAACAAGGAA CATTTCTTGT GTAGTGAGTG ACGGGTCAAC TGATGATTTC
    AACAAAGTGG TGGATGAAGA ATTCTGTACT GACATTGAAC CCATAATAGA TGGTAATAAA
    AAAATGGTTC TCGAGGAAAC CTGCACCAAG CCTTGCCCAG GTGACTGTTA TCTGAAGGAC
    TGGTCTTCCT GGAGCCTGTG TCAGCTAACC TGTGTGAATG GTGAAGATCT AGGCTTTGGT
    GGAATACAGG TCCGTTCCAG AGCTGTGATT ATACAAGAGC TAGAAAATCA GCATCTGTGC
    CCAGAGCAAA TGTTAGAAAC AAAATCATGT AATGATATGT ACCTTAAAGA AGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe16748
    Clone ID Related Accession (Same CDS sequence) XM_005628561.2 , XM_005628561.3
    Accession Version XM_005628561.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4497bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product thrombospondin type-1 domain-containing protein 7A isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006596.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005628561.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGGGCTGC AAGCGAGGCG CTCGGCGTCC CAGAGCCCCG GCGCCCCGGG CCCGCGCCGG 
    GGTGTCCTGC AGCTGCTGCC GCTGCTCCTG CTGCTGCTGC TGCTGCTGCT GCTGCGCCCC
    GGAGCCTGCG GGGCTGCGGC TCCCGGCGGG GCTGAGGCAC CCACCCTCTA CCTCTGGAAG
    ACTGGTCCAT GGGGCCGGTG TATGGGAGAT GAATGTGGTC CAGGAGGCAT TCAGACCCGG
    GCCGTGTGGT GTGCTCATGT GGAAGGATGG ACCACATTAC ATACAAACTG TAAGCAGGCT
    GAGAGACCCA GCAACCAGCA AAACTGTTTC AAGGTGTGTG ACTGGCACAA GGAGTTGTAT
    GATTGGAGGC TGGGACCTTG GAACCAGTGT CAGCCTGTGA TATCCAAGAG CCTGGAGAAA
    CCCCCTGAGT GCATTAAGGG GGAGGAAGGC ATCCAGGTGA GAGAAATAAC GTGCATCCAG
    AAAGAGAAGG ACATTCCTGC AGAGGATATC ATTTGTGAGT ACTTTGAGCC CAAACCTCTC
    CTGGAACAGG CCTGCCTCAT CCCCTGCCAG CAAGATTGCA TCGTGTCTGA GTTTTCCGCG
    TGGTCAGAAT GTTCCAAGAC TTGTGGCAGT GGGCTCCAGC ACCGGACTCG GCATGTGGTG
    GCGCCTCCCC AGTTTGGAGG TTCTGGCTGC CCAAACTTGA CAGAGTTTCA GGTGTGCCAG
    TCCAGCCCCT GTGAAGATGA AGAGAGCATG TACAGCTTGC ACGTGGGTCC CTGGAGCTCA
    TGCTCGATGT CCCACTCAAG ACAAGTAAGA CAAGCAAGGA GACGTGGGAA GAATAAAGAA
    CGGGAGAAGG ACCGAGGAAA AGGAGTGAAG GATCCCGAAG CCCGTGAGCT TATTAAGAAA
    AAGAGAAACA GAAACAGACA GAATCGACAA GAGAACAAAT ATTGGGACAT CCAGATTGGG
    TATCAGACCA GAGAGGTTAT GTGTACTAAC AAGACGGGAA AAGCTGTTGA TTTGAGCTTT
    TGCCAGCAAG AGAAGCTGCC AATGACCTTC CAGTCCTGTG TGATCACCAA AGAGTGCCAG
    GTGTCAGAGT GGTCAGAATG GAGTCCCTGC TCAAAAACAT GCCATGACAT GACATCCCCC
    AGAGGCACTC GCGTCAGGAC GCGGATCATT AGGCAGTTTC CTATTGGCAG CGAGAAAGAG
    TGTCCAGAAC TTGAAGAAAA AGAACCCTGT GTGTCTCAAG GAGATGCAGT TGCCCCCTGT
    GCCACGTATG GCTGGAGAAC TACAGAGTGG GCGGAGTGCC GTGTGGACCC CTTGCTCAGT
    CAGCAGGACA AGAGGCGGGG CAACCAGACA GCCCTCTGTG GAGGAGGAGT CCAGACACGA
    GAGGTGTACT GCGTGCAGGC CAATGAAAAC CTCCTCTCAC ACTTAAACAC CCACAAGGAC
    AAAGAAGAAA GTAAATATCA GAGTACGTCG AAGTCTAGTG CTGCACCTTT CCTATCCATC
    ACTTTTGAAA TAACAGCCTC AAAACCAGTG GACTTGAAAT CATGCACTGG CCCTGTCCCT
    AATACTACAC AGCTGTGCCA CATTCCCTGT CCTATTGAAT GTGAGGTTTC ACCTTGGTCA
    GCTTGGGGAC CTTGTACTTA TGAAAACTGT AATGACCAGC AAGGCAAAAA AGGTTTCAAA
    CTGAGGAAGC GACGCATTAC CAATGAGCCC ACTGGAGGCT CTGGGGGCAC TGGGAACTGC
    CCTCACTTAC TGGAAGCCAT TCCCTGTGAA GAGCCATCCT GCTATGAATG GAAAGCAGTG
    AGGCTTGGAG ACTGTGAACC AGACAATGGA AAAGAGTGTG GCCCAGGCAC TCAAGTTCAG
    GAGGTTGTAT GCATCAACAG TGATGGAGAA GAAGTTGACA GACAGCTGTG CAGAGATGCC
    ATCTTCCCCA TCCCCGTTGC CTGTTATGCC CCATGCCCAA AGGACTGTGT GCTCAGCACG
    TGGTCTGCAT GGTCCTCCTG CTCACACACC TGCTCGGGGA AAACCACAGA AGGGAAACAG
    ATACGAGCAC GGTCCATTCT GGCCTATGCC GGTGACGAAG GTGGAGCTCA CTGTCCAAAT
    AGCAGTGCTT TGCAAGAGGT GCGCAGTTGT AACGAGCATC CCTGCACTGT GTACCACTGG
    CAGACTGGTC CCTGGGGCCA GTGCATAGAG GACACTTCAG TATCATCCTT CAACACGACG
    ACTACTTGGA ATGGAGAGGC GTCTTGCTCT GTTGGCATGC AGACAAGAAA AGTCATTTGT
    GTGCGGGTCA ATGTGGGCCA AGTAGGACCC AAAAAGTGTC CTGAAAGCCT TAGACCTGAA
    ACAGTGAGGC CCTGTCTGCT TCCTTGTAGA AAGGACTGTA TTGTGACACC ATATAGTGAC
    TGGACATCAT GCCCCAACTC ATGTAAAGAA GGGGACTCCG GAGTCAGAAA GCAGTCTCGG
    CACCGGGTCA TCATTCAGCA GCCGGCCAAC GGGGGCAGGG ACTGCACGGA CCCGCTCTAC
    GAAGAGAAAG TCTGTGAGGC CCCTCAAGTG TGCCAAAGCT ACAGGTGGAA GACCCACAAA
    TGGCGCAGGT GCCAGTTGGT CCCTTGGAGC GTGCGACAGG ACAGCCCTGG AGCACAGGAA
    GGCTGCGGAC CCGGACGCCA GGCAAGAGCT ATTACTTGTC GAAAGCAAGA TGGAGGACAG
    GCTGGCATCC ATGAATGCCT CTGGTATGCA GGCCCTGTGC CAGCCCTCAC CCAGGCCTGC
    CAGATCCCAT GCCAAGATGA CTGTCAGTTT ACCAGCTGGT CCAAGTTTTC TTCCTGCAAC
    GGAGACTGTG GTGCAGTTAG GACCAGAAAA CGCACTATTG TTGGAAAAAG TAAAAAGAAG
    GAAAAATGTA AAAATTCTCA TTTGTACCCC CTGATTGAGA CTCAGTACTG TCCTTGTGAC
    AAGTATAATG CACAGCCTGT GGGGAACTGG TCAGACTGCA TTTTACCAGA GGGGAAAGTG
    GAAGTGTTGC TTGGAGTGAA AACACAAGGA GATACCAAGG AATGTGGTCA AGGATATCGT
    TACCAAGCAA TGGCATGCTA CGACCAAAAT GGCAGGCTCG TGGAAACATC CAGATGTAAC
    AGTCATGGTT ACATTGAAGA GGCCTGCATC ATCCCTTGTC CTTCTGATTG CAAGCTCAGT
    GAGTGGTCCA ACTGGTCTCG CTGCAGTAAG TCCTGTGGAA GTGGTGTGAA GGTTCGGTCT
    AAATGGTTGC GTGAGAAACC ATATAATGGA GGGAGGCCTT GCCCCAAGCT GGACCATGTC
    AACCAGGCAC AGGTGTATGA GGTTGTCCCT TGCCACAGCG ACTGCAACCA GTACATATGG
    GTCACAGAGC CATGGAGCAT CTGCAAAGTG ACCTTTGTCA ACATGCGAGA TAACTGTGGA
    GAGGGTGTGC AAACCCGAAA AGTGAGATGC ATGCAGAACA CAGCAGATGG CCCTTCTGAC
    CATGTAGAAG ATTACCTCTG CGATCCAGAA GAGATGCCCC TGGGCTCTAG AGAGTGCAAA
    TTACCATGCC CTGAGGATTG TGTGATATCT GAATGGAGTC CATGGACCCG CTGCACTTTG
    CCTTGCAGTC AAAGCAGTTT CCGGCAAAGG TCAGCTGATC CCATCAGACA ACCTGCAGAT
    GAAGGAAGAG CCTGCCCTAA TGCTGTTGAG AAGGAACCCT GTAACCTAAA CAAAAACTGC
    TTCCACTATG ATTATAATGT AACAGACTGG AGTACGTGTC AGCTGAGTGA GAAGGCAGTT
    TGTGGAAACG GCATTAAAAC AAGGATGTTG GATTGTGTTC GAAGCGATGG CAAGTCGGTT
    GACCTGAAAT ATTGTGAAGA GCTTGGCCTG GAGAAGACCT GGCAGATGAA CACATCCTGC
    ATGGTAGAAT GTCCCGTCAA CTGCCAGCTT TCCGACTGGT CTCCTTGGTC AGAATGTTCT
    CAAACATGTG GCCTCACAGG GAAAATGATC CGAAGACGCA CAGTGACCCA GCCCTTTCAA
    GGTGATGGAC GGCCATGCCC TGCCCTGATG GAACAGTCCA AGCCTTGCCC AGTGAAGCCC
    TGTTATCAGT GGCAATATGG CCAGTGGTCT CTATGCCAAG TGCAGGATGC CCAGTGTGGC
    GAAGGGACGA GAACAAGGAA CATTTCTTGT GTAGTGAGTG ACGGGTCAAC TGATGATTTC
    AACAAAGTGG TGGATGAAGA ATTCTGTACT GACATTGAAC CCATAATAGA TGGTAATAAA
    AAAATGGTTC TCGAGGAAAC CTGCACCAAG CCTTGCCCAG GTGACTGTTA TCTGAAGGAC
    TGGTCTTCCT GGAGCCTGTG TCAGCTAACC TGTGTGAATG GTGAAGATCT AGGCTTTGGT
    GGAATACAGG TCCGTTCCAG AGCTGTGATT ATACAAGAGC TAGAAAATCA GCATCTGTGC
    CCAGAGCAAA TGTTAGAAAC AAAATCATGT AATGATATGT ACCTTAAAGA AGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005628618.1
    CDS271..4767
    Translation

    Target ORF information:

    RefSeq Version XM_005628561.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris thrombospondin type 1 domain containing 7A (THSD7A), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005628561.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGGGCTGC AAGCGAGGCG CTCGGCGTCC CAGAGCCCCG GCGCCCCGGG CCCGCGCCGG 
    GGTGTCCTGC AGCTGCTGCC GCTGCTCCTG CTGCTGCTGC TGCTGCTGCT GCTGCGCCCC
    GGAGCCTGCG GGGCTGCGGC TCCCGGCGGG GCTGAGGCAC CCACCCTCTA CCTCTGGAAG
    ACTGGTCCAT GGGGCCGGTG TATGGGAGAT GAATGTGGTC CAGGAGGCAT TCAGACCCGG
    GCCGTGTGGT GTGCTCATGT GGAAGGATGG ACCACATTAC ATACAAACTG TAAGCAGGCT
    GAGAGACCCA GCAACCAGCA AAACTGTTTC AAGGTGTGTG ACTGGCACAA GGAGTTGTAT
    GATTGGAGGC TGGGACCTTG GAACCAGTGT CAGCCTGTGA TATCCAAGAG CCTGGAGAAA
    CCCCCTGAGT GCATTAAGGG GGAGGAAGGC ATCCAGGTGA GAGAAATAAC GTGCATCCAG
    AAAGAGAAGG ACATTCCTGC AGAGGATATC ATTTGTGAGT ACTTTGAGCC CAAACCTCTC
    CTGGAACAGG CCTGCCTCAT CCCCTGCCAG CAAGATTGCA TCGTGTCTGA GTTTTCCGCG
    TGGTCAGAAT GTTCCAAGAC TTGTGGCAGT GGGCTCCAGC ACCGGACTCG GCATGTGGTG
    GCGCCTCCCC AGTTTGGAGG TTCTGGCTGC CCAAACTTGA CAGAGTTTCA GGTGTGCCAG
    TCCAGCCCCT GTGAAGATGA AGAGAGCATG TACAGCTTGC ACGTGGGTCC CTGGAGCTCA
    TGCTCGATGT CCCACTCAAG ACAAGTAAGA CAAGCAAGGA GACGTGGGAA GAATAAAGAA
    CGGGAGAAGG ACCGAGGAAA AGGAGTGAAG GATCCCGAAG CCCGTGAGCT TATTAAGAAA
    AAGAGAAACA GAAACAGACA GAATCGACAA GAGAACAAAT ATTGGGACAT CCAGATTGGG
    TATCAGACCA GAGAGGTTAT GTGTACTAAC AAGACGGGAA AAGCTGTTGA TTTGAGCTTT
    TGCCAGCAAG AGAAGCTGCC AATGACCTTC CAGTCCTGTG TGATCACCAA AGAGTGCCAG
    GTGTCAGAGT GGTCAGAATG GAGTCCCTGC TCAAAAACAT GCCATGACAT GACATCCCCC
    AGAGGCACTC GCGTCAGGAC GCGGATCATT AGGCAGTTTC CTATTGGCAG CGAGAAAGAG
    TGTCCAGAAC TTGAAGAAAA AGAACCCTGT GTGTCTCAAG GAGATGCAGT TGCCCCCTGT
    GCCACGTATG GCTGGAGAAC TACAGAGTGG GCGGAGTGCC GTGTGGACCC CTTGCTCAGT
    CAGCAGGACA AGAGGCGGGG CAACCAGACA GCCCTCTGTG GAGGAGGAGT CCAGACACGA
    GAGGTGTACT GCGTGCAGGC CAATGAAAAC CTCCTCTCAC ACTTAAACAC CCACAAGGAC
    AAAGAAGAAA GTAAATATCA GAGTACGTCG AAGTCTAGTG CTGCACCTTT CCTATCCATC
    ACTTTTGAAA TAACAGCCTC AAAACCAGTG GACTTGAAAT CATGCACTGG CCCTGTCCCT
    AATACTACAC AGCTGTGCCA CATTCCCTGT CCTATTGAAT GTGAGGTTTC ACCTTGGTCA
    GCTTGGGGAC CTTGTACTTA TGAAAACTGT AATGACCAGC AAGGCAAAAA AGGTTTCAAA
    CTGAGGAAGC GACGCATTAC CAATGAGCCC ACTGGAGGCT CTGGGGGCAC TGGGAACTGC
    CCTCACTTAC TGGAAGCCAT TCCCTGTGAA GAGCCATCCT GCTATGAATG GAAAGCAGTG
    AGGCTTGGAG ACTGTGAACC AGACAATGGA AAAGAGTGTG GCCCAGGCAC TCAAGTTCAG
    GAGGTTGTAT GCATCAACAG TGATGGAGAA GAAGTTGACA GACAGCTGTG CAGAGATGCC
    ATCTTCCCCA TCCCCGTTGC CTGTTATGCC CCATGCCCAA AGGACTGTGT GCTCAGCACG
    TGGTCTGCAT GGTCCTCCTG CTCACACACC TGCTCGGGGA AAACCACAGA AGGGAAACAG
    ATACGAGCAC GGTCCATTCT GGCCTATGCC GGTGACGAAG GTGGAGCTCA CTGTCCAAAT
    AGCAGTGCTT TGCAAGAGGT GCGCAGTTGT AACGAGCATC CCTGCACTGT GTACCACTGG
    CAGACTGGTC CCTGGGGCCA GTGCATAGAG GACACTTCAG TATCATCCTT CAACACGACG
    ACTACTTGGA ATGGAGAGGC GTCTTGCTCT GTTGGCATGC AGACAAGAAA AGTCATTTGT
    GTGCGGGTCA ATGTGGGCCA AGTAGGACCC AAAAAGTGTC CTGAAAGCCT TAGACCTGAA
    ACAGTGAGGC CCTGTCTGCT TCCTTGTAGA AAGGACTGTA TTGTGACACC ATATAGTGAC
    TGGACATCAT GCCCCAACTC ATGTAAAGAA GGGGACTCCG GAGTCAGAAA GCAGTCTCGG
    CACCGGGTCA TCATTCAGCA GCCGGCCAAC GGGGGCAGGG ACTGCACGGA CCCGCTCTAC
    GAAGAGAAAG TCTGTGAGGC CCCTCAAGTG TGCCAAAGCT ACAGGTGGAA GACCCACAAA
    TGGCGCAGGT GCCAGTTGGT CCCTTGGAGC GTGCGACAGG ACAGCCCTGG AGCACAGGAA
    GGCTGCGGAC CCGGACGCCA GGCAAGAGCT ATTACTTGTC GAAAGCAAGA TGGAGGACAG
    GCTGGCATCC ATGAATGCCT CTGGTATGCA GGCCCTGTGC CAGCCCTCAC CCAGGCCTGC
    CAGATCCCAT GCCAAGATGA CTGTCAGTTT ACCAGCTGGT CCAAGTTTTC TTCCTGCAAC
    GGAGACTGTG GTGCAGTTAG GACCAGAAAA CGCACTATTG TTGGAAAAAG TAAAAAGAAG
    GAAAAATGTA AAAATTCTCA TTTGTACCCC CTGATTGAGA CTCAGTACTG TCCTTGTGAC
    AAGTATAATG CACAGCCTGT GGGGAACTGG TCAGACTGCA TTTTACCAGA GGGGAAAGTG
    GAAGTGTTGC TTGGAGTGAA AACACAAGGA GATACCAAGG AATGTGGTCA AGGATATCGT
    TACCAAGCAA TGGCATGCTA CGACCAAAAT GGCAGGCTCG TGGAAACATC CAGATGTAAC
    AGTCATGGTT ACATTGAAGA GGCCTGCATC ATCCCTTGTC CTTCTGATTG CAAGCTCAGT
    GAGTGGTCCA ACTGGTCTCG CTGCAGTAAG TCCTGTGGAA GTGGTGTGAA GGTTCGGTCT
    AAATGGTTGC GTGAGAAACC ATATAATGGA GGGAGGCCTT GCCCCAAGCT GGACCATGTC
    AACCAGGCAC AGGTGTATGA GGTTGTCCCT TGCCACAGCG ACTGCAACCA GTACATATGG
    GTCACAGAGC CATGGAGCAT CTGCAAAGTG ACCTTTGTCA ACATGCGAGA TAACTGTGGA
    GAGGGTGTGC AAACCCGAAA AGTGAGATGC ATGCAGAACA CAGCAGATGG CCCTTCTGAC
    CATGTAGAAG ATTACCTCTG CGATCCAGAA GAGATGCCCC TGGGCTCTAG AGAGTGCAAA
    TTACCATGCC CTGAGGATTG TGTGATATCT GAATGGAGTC CATGGACCCG CTGCACTTTG
    CCTTGCAGTC AAAGCAGTTT CCGGCAAAGG TCAGCTGATC CCATCAGACA ACCTGCAGAT
    GAAGGAAGAG CCTGCCCTAA TGCTGTTGAG AAGGAACCCT GTAACCTAAA CAAAAACTGC
    TTCCACTATG ATTATAATGT AACAGACTGG AGTACGTGTC AGCTGAGTGA GAAGGCAGTT
    TGTGGAAACG GCATTAAAAC AAGGATGTTG GATTGTGTTC GAAGCGATGG CAAGTCGGTT
    GACCTGAAAT ATTGTGAAGA GCTTGGCCTG GAGAAGACCT GGCAGATGAA CACATCCTGC
    ATGGTAGAAT GTCCCGTCAA CTGCCAGCTT TCCGACTGGT CTCCTTGGTC AGAATGTTCT
    CAAACATGTG GCCTCACAGG GAAAATGATC CGAAGACGCA CAGTGACCCA GCCCTTTCAA
    GGTGATGGAC GGCCATGCCC TGCCCTGATG GAACAGTCCA AGCCTTGCCC AGTGAAGCCC
    TGTTATCAGT GGCAATATGG CCAGTGGTCT CTATGCCAAG TGCAGGATGC CCAGTGTGGC
    GAAGGGACGA GAACAAGGAA CATTTCTTGT GTAGTGAGTG ACGGGTCAAC TGATGATTTC
    AACAAAGTGG TGGATGAAGA ATTCTGTACT GACATTGAAC CCATAATAGA TGGTAATAAA
    AAAATGGTTC TCGAGGAAAC CTGCACCAAG CCTTGCCCAG GTGACTGTTA TCTGAAGGAC
    TGGTCTTCCT GGAGCCTGTG TCAGCTAACC TGTGTGAATG GTGAAGATCT AGGCTTTGGT
    GGAATACAGG TCCGTTCCAG AGCTGTGATT ATACAAGAGC TAGAAAATCA GCATCTGTGC
    CCAGAGCAAA TGTTAGAAAC AAAATCATGT AATGATATGT ACCTTAAAGA AGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe293241
    Clone ID Related Accession (Same CDS sequence) XM_022427411.1
    Accession Version XM_022427411.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4908bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product thrombospondin type-1 domain-containing protein 7A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006596.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGGCTGC AAGCGAGGCG CTCGGCGTCC CAGAGCCCCG GCGCCCCGGG CCCGCGCCGG 
    GGTGTCCTGC AGCTGCTGCC GCTGCTCCTG CTGCTGCTGC TGCTGCTGCT GCTGCGCCCC
    GGAGCCTGCG GGGCTGCGGC TCCCGGCGGG GCTGAGGCAC CCACCCTCTA CCTCTGGAAG
    ACTGGTCCAT GGGGCCGGTG TATGGGAGAT GAATGTGGTC CAGGAGGCAT TCAGACCCGG
    GCCGTGTGGT GTGCTCATGT GGAAGGATGG ACCACATTAC ATACAAACTG TAAGCAGGCT
    GAGAGACCCA GCAACCAGCA AAACTGTTTC AAGGTGTGTG ACTGGCACAA GGAGTTGTAT
    GATTGGAGGC TGGGACCTTG GAACCAGTGT CAGCCTGTGA TATCCAAGAG CCTGGAGAAA
    CCCCCTGAGT GCATTAAGGG GGAGGAAGGC ATCCAGGTGA GAGAAATAAC GTGCATCCAG
    AAAGAGAAGG ACATTCCTGC AGAGGATATC ATTTGTGAGT ACTTTGAGCC CAAACCTCTC
    CTGGAACAGG CCTGCCTCAT CCCCTGCCAG CAAGATTGCA TCGTGTCTGA GTTTTCCGCG
    TGGTCAGAAT GTTCCAAGAC TTGTGGCAGT GGGCTCCAGC ACCGGACTCG GCATGTGGTG
    GCGCCTCCCC AGTTTGGAGG TTCTGGCTGC CCAAACTTGA CAGAGTTTCA GGTGTGCCAG
    TCCAGCCCCT GTGAAGATGA AGAGAGCATG TACAGCTTGC ACGTGGGTCC CTGGAGCTCA
    TGCTCGATGT CCCACTCAAG ACAAGTAAGA CAAGCAAGGA GACGTGGGAA GAATAAAGAA
    CGGGAGAAGG ACCGAGGAAA AGGAGTGAAG GATCCCGAAG CCCGTGAGCT TATTAAGAAA
    AAGAGAAACA GAAACAGACA GAATCGACAA GAGAACAAAT ATTGGGACAT CCAGATTGGG
    TATCAGACCA GAGAGGTTAT GTGTACTAAC AAGACGGGAA AAGCTGTTGA TTTGAGCTTT
    TGCCAGCAAG AGAAGCTGCC AATGACCTTC CAGTCCTGTG TGATCACCAA AGAGTGCCAG
    GTGTCAGAGT GGTCAGAATG GAGTCCCTGC TCAAAAACAT GCCATGACAT GACATCCCCC
    AGAGGCACTC GCGTCAGGAC GCGGATCATT AGGCAGTTTC CTATTGGCAG CGAGAAAGAG
    TGTCCAGAAC TTGAAGAAAA AGAACCCTGT GTGTCTCAAG GAGATGCAGT TGCCCCCTGT
    GCCACGTATG GCTGGAGAAC TACAGAGTGG GCGGAGTGCC GTGTGGACCC CTTGCTCAGT
    CAGCAGGACA AGAGGCGGGG CAACCAGACA GCCCTCTGTG GAGGAGGAGT CCAGACACGA
    GAGGTGTACT GCGTGCAGGC CAATGAAAAC CTCCTCTCAC ACTTAAACAC CCACAAGGAC
    AAAGAAGAAA GTAAATATCA GAGTACGTCG AAGTCTAGTG CTGCACCTTT CCTATCCATC
    ACTTTTGAAA TAACAGCCTC AAAACCAGTG GACTTGAAAT CATGCACTGG CCCTGTCCCT
    AATACTACAC AGCTGTGCCA CATTCCCTGT CCTATTGAAT GTGAGGTTTC ACCTTGGTCA
    GCTTGGGGAC CTTGTACTTA TGAAAACTGT AATGACCAGC AAGGCAAAAA AGGTTTCAAA
    CTGAGGAAGC GACGCATTAC CAATGAGCCC ACTGGAGGCT CTGGGGGCAC TGGGAACTGC
    CCTCACTTAC TGGAAGCCAT TCCCTGTGAA GAGCCATCCT GCTATGAATG GAAAGCAGTG
    AGGCTTGGAG ACTGTGAACC AGACAATGGA AAAGAGTGTG GCCCAGGCAC TCAAGTTCAG
    GAGGTTGTAT GCATCAACAG TGATGGAGAA GAAGTTGACA GACAGCTGTG CAGAGATGCC
    ATCTTCCCCA TCCCCGTTGC CTGTTATGCC CCATGCCCAA AGGACTGTGT GCTCAGCACG
    TGGTCTGCAT GGTCCTCCTG CTCACACACC TGCTCGGGGA AAACCACAGA AGGGAAACAG
    ATACGAGCAC GGTCCATTCT GGCCTATGCC GGTGACGAAG GTGGAGCTCA CTGTCCAAAT
    AGCAGTGCTT TGCAAGAGGT GCGCAGTTGT AACGAGCATC CCTGCACTGT GTACCACTGG
    CAGACTGGTC CCTGGGGCCA GTGCATAGAG GACACTTCAG TATCATCCTT CAACACGACG
    ACTACTTGGA ATGGAGAGGC GTCTTGCTCT GTTGGCATGC AGACAAGAAA AGTCATTTGT
    GTGCGGGTCA ATGTGGGCCA AGTAGGACCC AAAAAGTGTC CTGAAAGCCT TAGACCTGAA
    ACAGTGAGGC CCTGTCTGCT TCCTTGTAGA AAGGACTGTA TTGTGACACC ATATAGTGAC
    TGGACATCAT GCCCCAACTC ATGTAAAGAA GGGGACTCCG GAGTCAGAAA GCAGTCTCGG
    CACCGGGTCA TCATTCAGCA GCCGGCCAAC GGGGGCAGGG ACTGCACGGA CCCGCTCTAC
    GAAGAGAAAG TCTGTGAGGC CCCTCAAGTG TGCCAAAGCT ACAGGTGGAA GACCCACAAA
    TGGCGCAGGT GCCAGTTGGT CCCTTGGAGC GTGCGACAGG ACAGCCCTGG AGCACAGGAA
    GGCTGCGGAC CCGGACGCCA GGCAAGAGCT ATTACTTGTC GAAAGCAAGA TGGAGGACAG
    GCTGGCATCC ATGAATGCCT CTGGTATGCA GGCCCTGTGC CAGCCCTCAC CCAGGCCTGC
    CAGATCCCAT GCCAAGATGA CTGTCAGTTT ACCAGCTGGT CCAAGTTTTC TTCCTGCAAC
    GGAGACTGTG GTGCAGTTAG GACCAGAAAA CGCACTATTG TTGGAAAAAG TAAAAAGAAG
    GAAAAATGTA AAAATTCTCA TTTGTACCCC CTGATTGAGA CTCAGTACTG TCCTTGTGAC
    AAGTATAATG CACAGCCTGT GGGGAACTGG TCAGACTGCA TTTTACCAGA GGGGAAAGTG
    GAAGTGTTGC TTGGAGTGAA AACACAAGGA GATACCAAGG AATGTGGTCA AGGATATCGT
    TACCAAGCAA TGGCATGCTA CGACCAAAAT GGCAGGCTCG TGGAAACATC CAGATGTAAC
    AGTCATGGTT ACATTGAAGA GGCCTGCATC ATCCCTTGTC CTTCTGATTG CAAGCTCAGT
    GAGTGGTCCA ACTGGTCTCG CTGCAGTAAG TCCTGTGGAA GTGGTGTGAA GGTTCGGTCT
    AAATGGTTGC GTGAGAAACC ATATAATGGA GGGAGGCCTT GCCCCAAGCT GGACCATGTC
    AACCAGGCAC AGGTGTATGA GGTTGTCCCT TGCCACAGCG ACTGCAACCA GTACATATGG
    GTCACAGAGC CATGGAGCAT CTGCAAAGTG ACCTTTGTCA ACATGCGAGA TAACTGTGGA
    GAGGGTGTGC AAACCCGAAA AGTGAGATGC ATGCAGAACA CAGCAGATGG CCCTTCTGAC
    CATGTAGAAG ATTACCTCTG CGATCCAGAA GAGATGCCCC TGGGCTCTAG AGAGTGCAAA
    TTACCATGCC CTGAGGATTG TGTGATATCT GAATGGAGTC CATGGACCCG CTGCACTTTG
    CCTTGCAGTC AAAGCAGTTT CCGGCAAAGG TCAGCTGATC CCATCAGACA ACCTGCAGAT
    GAAGGAAGAG CCTGCCCTAA TGCTGTTGAG AAGGAACCCT GTAACCTAAA CAAAAACTGC
    TTCCACTATG ATTATAATGT AACAGACTGG AGTACGTGTC AGCTGAGTGA GAAGGCAGTT
    TGTGGAAACG GCATTAAAAC AAGGATGTTG GATTGTGTTC GAAGCGATGG CAAGTCGGTT
    GACCTGAAAT ATTGTGAAGA GCTTGGCCTG GAGAAGACCT GGCAGATGAA CACATCCTGC
    ATGGTAGAAT GTCCCGTCAA CTGCCAGCTT TCCGACTGGT CTCCTTGGTC AGAATGTTCT
    CAAACATGTG GCCTCACAGG GAAAATGATC CGAAGACGCA CAGTGACCCA GCCCTTTCAA
    GGTGATGGAC GGCCATGCCC TGCCCTGATG GAACAGTCCA AGCCTTGCCC AGTGAAGCCC
    TGTTATCAGT GGCAATATGG CCAGTGGTCT CTATGCCAAG TGCAGGATGC CCAGTGTGGC
    GAAGGGACGA GAACAAGGAA CATTTCTTGT GTAGTGAGTG ACGGGTCAAC TGATGATTTC
    AACAAAGTGG TGGATGAAGA ATTCTGTACT GACATTGAAC CCATAATAGA TGGTAATAAA
    AAAATGGTTC TCGAGGAAAC CTGCACCAAG CCTTGCCCAG GTGACTGTTA TCTGAAGGAC
    TGGTCTTCCT GGAGCCTGTG TCAGCTAACC TGTGTGAATG GTGAAGATCT AGGCTTTGGT
    GGAATACAGG TCCGTTCCAG AGCTGTGATT ATACAAGAGC TAGAAAATCA GCATCTGTGC
    CCAGAGCAAA TGTTAGAAAC AAAATCATGT AATGATGGAC AGTGTTATGA GTATAAATGG
    ATGGCCAGTG CTTGGAAGGG TTCTTCCCGA ACAGTCTGGT GTCAAAGGTC AGATGGTATA
    AATGTAACAG GGGGCTGCTT GGTGGTGCGC CAGCCTGATG CTGACAGGTC TTGTAACCCA
    CCGTGTAGTC AACCCCACTC GTACTGTAGT GAGATGAGGA CATGCAGTTG TGAAGAAGGG
    TATACTGAAG TCATGTCTTC TAACGGTACA CTTGAGCAAT GCACACTTAT CCCCGTGGTG
    GTGATACCCA CTGTGGAGGA CAAAAGAGGA GATGTGAAAA CCAGTCGGGC AGTACATCCA
    ACCCAATCCT CCAGTAATCC AACAGGACGG GGAAGGACCT GGTTTCTACA GCCATTTGGG
    CCAGCAAAAA GCCAAAGAAA CCCCAAAGAA GGCAAAACAA CCGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022283119.1
    CDS270..5177
    Translation

    Target ORF information:

    RefSeq Version XM_022427411.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris thrombospondin type 1 domain containing 7A (THSD7A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022427411.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGGCTGC AAGCGAGGCG CTCGGCGTCC CAGAGCCCCG GCGCCCCGGG CCCGCGCCGG 
    GGTGTCCTGC AGCTGCTGCC GCTGCTCCTG CTGCTGCTGC TGCTGCTGCT GCTGCGCCCC
    GGAGCCTGCG GGGCTGCGGC TCCCGGCGGG GCTGAGGCAC CCACCCTCTA CCTCTGGAAG
    ACTGGTCCAT GGGGCCGGTG TATGGGAGAT GAATGTGGTC CAGGAGGCAT TCAGACCCGG
    GCCGTGTGGT GTGCTCATGT GGAAGGATGG ACCACATTAC ATACAAACTG TAAGCAGGCT
    GAGAGACCCA GCAACCAGCA AAACTGTTTC AAGGTGTGTG ACTGGCACAA GGAGTTGTAT
    GATTGGAGGC TGGGACCTTG GAACCAGTGT CAGCCTGTGA TATCCAAGAG CCTGGAGAAA
    CCCCCTGAGT GCATTAAGGG GGAGGAAGGC ATCCAGGTGA GAGAAATAAC GTGCATCCAG
    AAAGAGAAGG ACATTCCTGC AGAGGATATC ATTTGTGAGT ACTTTGAGCC CAAACCTCTC
    CTGGAACAGG CCTGCCTCAT CCCCTGCCAG CAAGATTGCA TCGTGTCTGA GTTTTCCGCG
    TGGTCAGAAT GTTCCAAGAC TTGTGGCAGT GGGCTCCAGC ACCGGACTCG GCATGTGGTG
    GCGCCTCCCC AGTTTGGAGG TTCTGGCTGC CCAAACTTGA CAGAGTTTCA GGTGTGCCAG
    TCCAGCCCCT GTGAAGATGA AGAGAGCATG TACAGCTTGC ACGTGGGTCC CTGGAGCTCA
    TGCTCGATGT CCCACTCAAG ACAAGTAAGA CAAGCAAGGA GACGTGGGAA GAATAAAGAA
    CGGGAGAAGG ACCGAGGAAA AGGAGTGAAG GATCCCGAAG CCCGTGAGCT TATTAAGAAA
    AAGAGAAACA GAAACAGACA GAATCGACAA GAGAACAAAT ATTGGGACAT CCAGATTGGG
    TATCAGACCA GAGAGGTTAT GTGTACTAAC AAGACGGGAA AAGCTGTTGA TTTGAGCTTT
    TGCCAGCAAG AGAAGCTGCC AATGACCTTC CAGTCCTGTG TGATCACCAA AGAGTGCCAG
    GTGTCAGAGT GGTCAGAATG GAGTCCCTGC TCAAAAACAT GCCATGACAT GACATCCCCC
    AGAGGCACTC GCGTCAGGAC GCGGATCATT AGGCAGTTTC CTATTGGCAG CGAGAAAGAG
    TGTCCAGAAC TTGAAGAAAA AGAACCCTGT GTGTCTCAAG GAGATGCAGT TGCCCCCTGT
    GCCACGTATG GCTGGAGAAC TACAGAGTGG GCGGAGTGCC GTGTGGACCC CTTGCTCAGT
    CAGCAGGACA AGAGGCGGGG CAACCAGACA GCCCTCTGTG GAGGAGGAGT CCAGACACGA
    GAGGTGTACT GCGTGCAGGC CAATGAAAAC CTCCTCTCAC ACTTAAACAC CCACAAGGAC
    AAAGAAGAAA GTAAATATCA GAGTACGTCG AAGTCTAGTG CTGCACCTTT CCTATCCATC
    ACTTTTGAAA TAACAGCCTC AAAACCAGTG GACTTGAAAT CATGCACTGG CCCTGTCCCT
    AATACTACAC AGCTGTGCCA CATTCCCTGT CCTATTGAAT GTGAGGTTTC ACCTTGGTCA
    GCTTGGGGAC CTTGTACTTA TGAAAACTGT AATGACCAGC AAGGCAAAAA AGGTTTCAAA
    CTGAGGAAGC GACGCATTAC CAATGAGCCC ACTGGAGGCT CTGGGGGCAC TGGGAACTGC
    CCTCACTTAC TGGAAGCCAT TCCCTGTGAA GAGCCATCCT GCTATGAATG GAAAGCAGTG
    AGGCTTGGAG ACTGTGAACC AGACAATGGA AAAGAGTGTG GCCCAGGCAC TCAAGTTCAG
    GAGGTTGTAT GCATCAACAG TGATGGAGAA GAAGTTGACA GACAGCTGTG CAGAGATGCC
    ATCTTCCCCA TCCCCGTTGC CTGTTATGCC CCATGCCCAA AGGACTGTGT GCTCAGCACG
    TGGTCTGCAT GGTCCTCCTG CTCACACACC TGCTCGGGGA AAACCACAGA AGGGAAACAG
    ATACGAGCAC GGTCCATTCT GGCCTATGCC GGTGACGAAG GTGGAGCTCA CTGTCCAAAT
    AGCAGTGCTT TGCAAGAGGT GCGCAGTTGT AACGAGCATC CCTGCACTGT GTACCACTGG
    CAGACTGGTC CCTGGGGCCA GTGCATAGAG GACACTTCAG TATCATCCTT CAACACGACG
    ACTACTTGGA ATGGAGAGGC GTCTTGCTCT GTTGGCATGC AGACAAGAAA AGTCATTTGT
    GTGCGGGTCA ATGTGGGCCA AGTAGGACCC AAAAAGTGTC CTGAAAGCCT TAGACCTGAA
    ACAGTGAGGC CCTGTCTGCT TCCTTGTAGA AAGGACTGTA TTGTGACACC ATATAGTGAC
    TGGACATCAT GCCCCAACTC ATGTAAAGAA GGGGACTCCG GAGTCAGAAA GCAGTCTCGG
    CACCGGGTCA TCATTCAGCA GCCGGCCAAC GGGGGCAGGG ACTGCACGGA CCCGCTCTAC
    GAAGAGAAAG TCTGTGAGGC CCCTCAAGTG TGCCAAAGCT ACAGGTGGAA GACCCACAAA
    TGGCGCAGGT GCCAGTTGGT CCCTTGGAGC GTGCGACAGG ACAGCCCTGG AGCACAGGAA
    GGCTGCGGAC CCGGACGCCA GGCAAGAGCT ATTACTTGTC GAAAGCAAGA TGGAGGACAG
    GCTGGCATCC ATGAATGCCT CTGGTATGCA GGCCCTGTGC CAGCCCTCAC CCAGGCCTGC
    CAGATCCCAT GCCAAGATGA CTGTCAGTTT ACCAGCTGGT CCAAGTTTTC TTCCTGCAAC
    GGAGACTGTG GTGCAGTTAG GACCAGAAAA CGCACTATTG TTGGAAAAAG TAAAAAGAAG
    GAAAAATGTA AAAATTCTCA TTTGTACCCC CTGATTGAGA CTCAGTACTG TCCTTGTGAC
    AAGTATAATG CACAGCCTGT GGGGAACTGG TCAGACTGCA TTTTACCAGA GGGGAAAGTG
    GAAGTGTTGC TTGGAGTGAA AACACAAGGA GATACCAAGG AATGTGGTCA AGGATATCGT
    TACCAAGCAA TGGCATGCTA CGACCAAAAT GGCAGGCTCG TGGAAACATC CAGATGTAAC
    AGTCATGGTT ACATTGAAGA GGCCTGCATC ATCCCTTGTC CTTCTGATTG CAAGCTCAGT
    GAGTGGTCCA ACTGGTCTCG CTGCAGTAAG TCCTGTGGAA GTGGTGTGAA GGTTCGGTCT
    AAATGGTTGC GTGAGAAACC ATATAATGGA GGGAGGCCTT GCCCCAAGCT GGACCATGTC
    AACCAGGCAC AGGTGTATGA GGTTGTCCCT TGCCACAGCG ACTGCAACCA GTACATATGG
    GTCACAGAGC CATGGAGCAT CTGCAAAGTG ACCTTTGTCA ACATGCGAGA TAACTGTGGA
    GAGGGTGTGC AAACCCGAAA AGTGAGATGC ATGCAGAACA CAGCAGATGG CCCTTCTGAC
    CATGTAGAAG ATTACCTCTG CGATCCAGAA GAGATGCCCC TGGGCTCTAG AGAGTGCAAA
    TTACCATGCC CTGAGGATTG TGTGATATCT GAATGGAGTC CATGGACCCG CTGCACTTTG
    CCTTGCAGTC AAAGCAGTTT CCGGCAAAGG TCAGCTGATC CCATCAGACA ACCTGCAGAT
    GAAGGAAGAG CCTGCCCTAA TGCTGTTGAG AAGGAACCCT GTAACCTAAA CAAAAACTGC
    TTCCACTATG ATTATAATGT AACAGACTGG AGTACGTGTC AGCTGAGTGA GAAGGCAGTT
    TGTGGAAACG GCATTAAAAC AAGGATGTTG GATTGTGTTC GAAGCGATGG CAAGTCGGTT
    GACCTGAAAT ATTGTGAAGA GCTTGGCCTG GAGAAGACCT GGCAGATGAA CACATCCTGC
    ATGGTAGAAT GTCCCGTCAA CTGCCAGCTT TCCGACTGGT CTCCTTGGTC AGAATGTTCT
    CAAACATGTG GCCTCACAGG GAAAATGATC CGAAGACGCA CAGTGACCCA GCCCTTTCAA
    GGTGATGGAC GGCCATGCCC TGCCCTGATG GAACAGTCCA AGCCTTGCCC AGTGAAGCCC
    TGTTATCAGT GGCAATATGG CCAGTGGTCT CTATGCCAAG TGCAGGATGC CCAGTGTGGC
    GAAGGGACGA GAACAAGGAA CATTTCTTGT GTAGTGAGTG ACGGGTCAAC TGATGATTTC
    AACAAAGTGG TGGATGAAGA ATTCTGTACT GACATTGAAC CCATAATAGA TGGTAATAAA
    AAAATGGTTC TCGAGGAAAC CTGCACCAAG CCTTGCCCAG GTGACTGTTA TCTGAAGGAC
    TGGTCTTCCT GGAGCCTGTG TCAGCTAACC TGTGTGAATG GTGAAGATCT AGGCTTTGGT
    GGAATACAGG TCCGTTCCAG AGCTGTGATT ATACAAGAGC TAGAAAATCA GCATCTGTGC
    CCAGAGCAAA TGTTAGAAAC AAAATCATGT AATGATGGAC AGTGTTATGA GTATAAATGG
    ATGGCCAGTG CTTGGAAGGG TTCTTCCCGA ACAGTCTGGT GTCAAAGGTC AGATGGTATA
    AATGTAACAG GGGGCTGCTT GGTGGTGCGC CAGCCTGATG CTGACAGGTC TTGTAACCCA
    CCGTGTAGTC AACCCCACTC GTACTGTAGT GAGATGAGGA CATGCAGTTG TGAAGAAGGG
    TATACTGAAG TCATGTCTTC TAACGGTACA CTTGAGCAAT GCACACTTAT CCCCGTGGTG
    GTGATACCCA CTGTGGAGGA CAAAAGAGGA GATGTGAAAA CCAGTCGGGC AGTACATCCA
    ACCCAATCCT CCAGTAATCC AACAGGACGG GGAAGGACCT GGTTTCTACA GCCATTTGGG
    CCAGCAAAAA GCCAAAGAAA CCCCAAAGAA GGCAAAACAA CCGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe293242
    Clone ID Related Accession (Same CDS sequence) XM_022427412.1
    Accession Version XM_022427412.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product thrombospondin type-1 domain-containing protein 7A isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006596.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGGCTGC AAGCGAGGCG CTCGGCGTCC CAGAGCCCCG GCGCCCCGGG CCCGCGCCGG 
    GGTGTCCTGC AGCTGCTGCC GCTGCTCCTG CTGCTGCTGC TGCTGCTGCT GCTGCGCCCC
    GGAGCCTGCG GGGCTGCGGC TCCCGGCGGG GCTGAGGCAC CCACCCTCTA CCTCTGGAAG
    ACTGGTCCAT GGGGCCGGTG TATGGGAGAT GAATGTGGTC CAGGAGGCAT TCAGACCCGG
    GCCGTGTGGT GTGCTCATGT GGAAGGATGG ACCACATTAC ATACAAACTG TAAGCAGGCT
    GAGAGACCCA GCAACCAGCA AAACTGTTTC AAGGTGTGTG ACTGGCACAA GGAGTTGTAT
    GATTGGAGGC TGGGACCTTG GAACCAGTGT CAGCCTGTGA TATCCAAGAG CCTGGAGAAA
    CCCCCTGAGT GCATTAAGGG GGAGGAAGGC ATCCAGGTGA GAGAAATAAC GTGCATCCAG
    AAAGAGAAGG ACATTCCTGC AGAGGATATC ATTTGTGAGT ACTTTGAGCC CAAACCTCTC
    CTGGAACAGG CCTGCCTCAT CCCCTGCCAG CAAGATTGCA TCGTGTCTGA GTTTTCCGCG
    TGGTCAGAAT GTTCCAAGAC TTGTGGCAGT GGGCTCCAGC ACCGGACTCG GCATGTGGTG
    GCGCCTCCCC AGTTTGGAGG TTCTGGCTGC CCAAACTTGA CAGAGTTTCA GGTGTGCCAG
    TCCAGCCCCT GTGAAGATGA AGAGAGCATG TACAGCTTGC ACGTGGGTCC CTGGAGCTCA
    TGCTCGATGT CCCACTCAAG ACAAGTAAGA CAAGCAAGGA GACGTGGGAA GAATAAAGAA
    CGGGAGAAGG ACCGAGGAAA AGGAGTGAAG GATCCCGAAG CCCGTGAGCT TATTAAGAAA
    AAGAGAAACA GAAACAGACA GAATCGACAA GAGAACAAAT ATTGGGACAT CCAGATTGGG
    TATCAGACCA GAGAGGTTAT GTGTACTAAC AAGACGGGAA AAGCTGTTGA TTTGAGCTTT
    TGCCAGCAAG AGAAGCTGCC AATGACCTTC CAGTCCTGTG TGATCACCAA AGAGTGCCAG
    GTGTCAGAGT GGTCAGAATG GAGTCCCTGC TCAAAAACAT GCCATGACAT GACATCCCCC
    AGAGGCACTC GCGTCAGGAC GCGGATCATT AGGCAGTTTC CTATTGGCAG CGAGAAAGAG
    TGTCCAGAAC TTGAAGAAAA AGAACCCTGT GTGTCTCAAG GAGATGCAGT TGCCCCCTGT
    GCCACGTATG GCTGGAGAAC TACAGAGTGG GCGGAGTGCC GTGTGGACCC CTTGCTCAGT
    CAGCAGGACA AGAGGCGGGG CAACCAGACA GCCCTCTGTG GAGGAGGAGT CCAGACACGA
    GAGGTGTACT GCGTGCAGGC CAATGAAAAC CTCCTCTCAC ACTTAAACAC CCACAAGGAC
    AAAGAAGAAA GTAAATATCA GAGTACGTCG AAGTCTAGTG CTGCACCTTT CCTATCCATC
    ACTTTTGAAA TAACAGCCTC AAAACCAGTG GACTTGAAAT CATGCACTGG CCCTGTCCCT
    AATACTACAC AGCTGTGCCA CATTCCCTGT CCTATTGAAT GTGAGGTTTC ACCTTGGTCA
    GCTTGGGGAC CTTGTACTTA TGAAAACTGT AATGACCAGC AAGGCAAAAA AGGTTTCAAA
    CTGAGGAAGC GACGCATTAC CAATGAGCCC ACTGGAGGCT CTGGGGGCAC TGGGAACTGC
    CCTCACTTAC TGGAAGCCAT TCCCTGTGAA GAGCCATCCT GCTATGAATG GAAAGCAGTG
    AGGCTTGGAG ACTGTGAACC AGACAATGGA AAAGAGTGTG GCCCAGGCAC TCAAGTTCAG
    GAGGTTGTAT GCATCAACAG TGATGGAGAA GAAGTTGACA GACAGCTGTG CAGAGATGCC
    ATCTTCCCCA TCCCCGTTGC CTGTTATGCC CCATGCCCAA AGGACTGTGT GCTCAGCACG
    TGGTCTGCAT GGTCCTCCTG CTCACACACC TGCTCGGGGA AAACCACAGA AGGGAAACAG
    ATACGAGCAC GGTCCATTCT GGCCTATGCC GGTGACGAAG GTGGAGCTCA CTGTCCAAAT
    AGCAGTGCTT TGCAAGAGGT GCGCAGTTGT AACGAGCATC CCTGCACTGT GTACCACTGG
    CAGACTGGTC CCTGGGGCCA GTGCATAGAG GACACTTCAG TATCATCCTT CAACACGACG
    ACTACTTGGA ATGGAGAGGC GTCTTGCTCT GTTGGCATGC AGACAAGAAA AGTCATTTGT
    GTGCGGGTCA ATGTGGGCCA AGTAGGACCC AAAAAGTGTC CTGAAAGCCT TAGACCTGAA
    ACAGTGAGGC CCTGTCTGCT TCCTTGTAGA AAGGACTGTA TTGTGACACC ATATAGTGAC
    TGGACATCAT GCCCCAACTC ATGTAAAGAA GGGGACTCCG GAGTCAGAAA GCAGTCTCGG
    CACCGGGTCA TCATTCAGCA GCCGGCCAAC GGGGGCAGGG ACTGCACGGA CCCGCTCTAC
    GAAGAGAAAG TCTGTGAGGC CCCTCAAGTG TGCCAAAGCT ACAGGTGGAA GACCCACAAA
    TGGCGCAGGT GCCAGTTGGT CCCTTGGAGC GTGCGACAGG ACAGCCCTGG AGCACAGGAA
    GGCTGCGGAC CCGGACGCCA GGCAAGAGCT ATTACTTGTC GAAAGCAAGA TGGAGGACAG
    GCTGGCATCC ATGAATGCCT CTGGTATGCA GGCCCTGTGC CAGCCCTCAC CCAGGCCTGC
    CAGATCCCAT GCCAAGATGA CTGTCAGTTT ACCAGCTGGT CCAAGTTTTC TTCCTGCAAC
    GGAGACTGTG GTGCAGTTAG GACCAGAAAA CGCACTATTG TTGGAAAAAG TAAAAAGAAG
    GAAAAATGTA AAAATTCTCA TTTGTACCCC CTGATTGAGA CTCAGTACTG TCCTTGTGAC
    AAGTATAATG CACAGCCTGT GGGGAACTGG TCAGACTGCA TTTTACCAGA GGGGAAAGTG
    GAAGTGTTGC TTGGAGTGAA AACACAAGGA GATACCAAGG AATGTGGTCA AGGATATCGT
    TACCAAGCAA TGGCATGCTA CGACCAAAAT GGCAGGCTCG TGGAAACATC CAGATGTAAC
    AGTCATGGTT ACATTGAAGA GGCCTGCATC ATCCCTTGTC CTTCTGATTG CAAGCTCAGT
    GAGTGGTCCA ACTGGTCTCG CTGCAGTAAG TCCTGTGGAA GTGGTGTGAA GGTTCGGTCT
    AAATGGTTGC GTGAGAAACC ATATAATGGA GGGAGGCCTT GCCCCAAGCT GGACCATGTC
    AACCAGGCAC AGGTGTATGA GGTTGTCCCT TGCCACAGCG ACTGCAACCA GTACATATGG
    GTCACAGAGC CATGGAGCAT CTGCAAAGTG ACCTTTGTCA ACATGCGAGA TAACTGTGGA
    GAGGGTGTGC AAACCCGAAA AGTGAGATGC ATGCAGAACA CAGCAGATGG CCCTTCTGAC
    CATGTAGAAG ATTACCTCTG CGATCCAGAA GAGATGCCCC TGGGCTCTAG AGAGTGCAAA
    TTACCATGCC CTGAGGATTG TGTGATATCT GAATGGAGTC CATGGACCCG CTGCACTTTG
    CCTTGCAGTC AAAGCAGTTT CCGGCAAAGG TCAGCTGATC CCATCAGACA ACCTGCAGAT
    GAAGGAAGAG CCTGCCCTAA TGCTGTTGAG AAGGAACCCT GTAACCTAAA CAAAAACTGC
    TTCCACTATG ATTATAATGT AACAGACTGG AGTACGTGTC AGCTGAGTGA GAAGGCAGTT
    TGTGGAAACG GCATTAAAAC AAGGATGTTG GATTGTGTTC GAAGCGATGG CAAGTCGGTT
    GACCTGAAAT ATTGTGAAGA GCTTGGCCTG GAGAAGACCT GGCAGATGAA CACATCCTGC
    ATGGTAGAAT GTCCCGTCAA CTGCCAGCTT TCCGACTGGT CTCCTTGGTC AGAATGTTCT
    CAAACATGTG GCCTCACAGG GAAAATGATC CGAAGACGCA CAGTGACCCA GCCCTTTCAA
    GGTGATGGAC GGCCATGCCC TGCCCTGATG GAACAGTCCA AGCCTTGCCC AGTGAAGCCC
    TGTTATCAGT GGCAATATGG CCAGTGGTCT CTATGCCAAG TGCAGGATGC CCAGTGTGGC
    GAAGGGACGA GAACAAGGAA CATTTCTTGT GTAGTGAGTG ACGGGTCAAC TGATGATTTC
    AACAAAGTGG TGGATGAAGA ATTCTGTACT GACATTGAAC CCATAATAGA TGGTAATAAA
    AAAATGGTTC TCGAGGAAAC CTGCACCAAG CCTTGCCCAG GTGACTGTTA TCTGAAGGAC
    TGGTCTTCCT GGAGCCTGTG TCAGCTAACC TGTGTGAATG GTGAAGATCT AGGCTTTGGT
    GGAATACAGG TCCGTTCCAG AGCTGTGATT ATACAAGAGC TAGAAAATCA GCATCTGTGC
    CCAGAGCAAA TGTTAGAAAC AAAATCATGT AATGATGGAC AGTGTTATGA GTATAAATGG
    ATGGCCAGTG CTTGGAAGGG TTCTTCCCGA ACAGTCTGGT GTCAAAGGTC AGATGGTATA
    AATGTAACAG ATGGGAGACT AAAGACCTGG GTTTACGGTG TAGCAGCTGG GGCATTTGTG
    TTACTCATCT TTATTGTCTC CATGATTTAT CTAGCTTGCA AAAAGCCAAA GAAACCCCAA
    AGAAGGCAAA ACAACCGACT GAAACCTTTA ACCCTAGCCT ACGATGGAGA TGCAGACATG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022283120.1
    CDS270..5012
    Translation

    Target ORF information:

    RefSeq Version XM_022427412.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris thrombospondin type 1 domain containing 7A (THSD7A), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022427412.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGGCTGC AAGCGAGGCG CTCGGCGTCC CAGAGCCCCG GCGCCCCGGG CCCGCGCCGG 
    GGTGTCCTGC AGCTGCTGCC GCTGCTCCTG CTGCTGCTGC TGCTGCTGCT GCTGCGCCCC
    GGAGCCTGCG GGGCTGCGGC TCCCGGCGGG GCTGAGGCAC CCACCCTCTA CCTCTGGAAG
    ACTGGTCCAT GGGGCCGGTG TATGGGAGAT GAATGTGGTC CAGGAGGCAT TCAGACCCGG
    GCCGTGTGGT GTGCTCATGT GGAAGGATGG ACCACATTAC ATACAAACTG TAAGCAGGCT
    GAGAGACCCA GCAACCAGCA AAACTGTTTC AAGGTGTGTG ACTGGCACAA GGAGTTGTAT
    GATTGGAGGC TGGGACCTTG GAACCAGTGT CAGCCTGTGA TATCCAAGAG CCTGGAGAAA
    CCCCCTGAGT GCATTAAGGG GGAGGAAGGC ATCCAGGTGA GAGAAATAAC GTGCATCCAG
    AAAGAGAAGG ACATTCCTGC AGAGGATATC ATTTGTGAGT ACTTTGAGCC CAAACCTCTC
    CTGGAACAGG CCTGCCTCAT CCCCTGCCAG CAAGATTGCA TCGTGTCTGA GTTTTCCGCG
    TGGTCAGAAT GTTCCAAGAC TTGTGGCAGT GGGCTCCAGC ACCGGACTCG GCATGTGGTG
    GCGCCTCCCC AGTTTGGAGG TTCTGGCTGC CCAAACTTGA CAGAGTTTCA GGTGTGCCAG
    TCCAGCCCCT GTGAAGATGA AGAGAGCATG TACAGCTTGC ACGTGGGTCC CTGGAGCTCA
    TGCTCGATGT CCCACTCAAG ACAAGTAAGA CAAGCAAGGA GACGTGGGAA GAATAAAGAA
    CGGGAGAAGG ACCGAGGAAA AGGAGTGAAG GATCCCGAAG CCCGTGAGCT TATTAAGAAA
    AAGAGAAACA GAAACAGACA GAATCGACAA GAGAACAAAT ATTGGGACAT CCAGATTGGG
    TATCAGACCA GAGAGGTTAT GTGTACTAAC AAGACGGGAA AAGCTGTTGA TTTGAGCTTT
    TGCCAGCAAG AGAAGCTGCC AATGACCTTC CAGTCCTGTG TGATCACCAA AGAGTGCCAG
    GTGTCAGAGT GGTCAGAATG GAGTCCCTGC TCAAAAACAT GCCATGACAT GACATCCCCC
    AGAGGCACTC GCGTCAGGAC GCGGATCATT AGGCAGTTTC CTATTGGCAG CGAGAAAGAG
    TGTCCAGAAC TTGAAGAAAA AGAACCCTGT GTGTCTCAAG GAGATGCAGT TGCCCCCTGT
    GCCACGTATG GCTGGAGAAC TACAGAGTGG GCGGAGTGCC GTGTGGACCC CTTGCTCAGT
    CAGCAGGACA AGAGGCGGGG CAACCAGACA GCCCTCTGTG GAGGAGGAGT CCAGACACGA
    GAGGTGTACT GCGTGCAGGC CAATGAAAAC CTCCTCTCAC ACTTAAACAC CCACAAGGAC
    AAAGAAGAAA GTAAATATCA GAGTACGTCG AAGTCTAGTG CTGCACCTTT CCTATCCATC
    ACTTTTGAAA TAACAGCCTC AAAACCAGTG GACTTGAAAT CATGCACTGG CCCTGTCCCT
    AATACTACAC AGCTGTGCCA CATTCCCTGT CCTATTGAAT GTGAGGTTTC ACCTTGGTCA
    GCTTGGGGAC CTTGTACTTA TGAAAACTGT AATGACCAGC AAGGCAAAAA AGGTTTCAAA
    CTGAGGAAGC GACGCATTAC CAATGAGCCC ACTGGAGGCT CTGGGGGCAC TGGGAACTGC
    CCTCACTTAC TGGAAGCCAT TCCCTGTGAA GAGCCATCCT GCTATGAATG GAAAGCAGTG
    AGGCTTGGAG ACTGTGAACC AGACAATGGA AAAGAGTGTG GCCCAGGCAC TCAAGTTCAG
    GAGGTTGTAT GCATCAACAG TGATGGAGAA GAAGTTGACA GACAGCTGTG CAGAGATGCC
    ATCTTCCCCA TCCCCGTTGC CTGTTATGCC CCATGCCCAA AGGACTGTGT GCTCAGCACG
    TGGTCTGCAT GGTCCTCCTG CTCACACACC TGCTCGGGGA AAACCACAGA AGGGAAACAG
    ATACGAGCAC GGTCCATTCT GGCCTATGCC GGTGACGAAG GTGGAGCTCA CTGTCCAAAT
    AGCAGTGCTT TGCAAGAGGT GCGCAGTTGT AACGAGCATC CCTGCACTGT GTACCACTGG
    CAGACTGGTC CCTGGGGCCA GTGCATAGAG GACACTTCAG TATCATCCTT CAACACGACG
    ACTACTTGGA ATGGAGAGGC GTCTTGCTCT GTTGGCATGC AGACAAGAAA AGTCATTTGT
    GTGCGGGTCA ATGTGGGCCA AGTAGGACCC AAAAAGTGTC CTGAAAGCCT TAGACCTGAA
    ACAGTGAGGC CCTGTCTGCT TCCTTGTAGA AAGGACTGTA TTGTGACACC ATATAGTGAC
    TGGACATCAT GCCCCAACTC ATGTAAAGAA GGGGACTCCG GAGTCAGAAA GCAGTCTCGG
    CACCGGGTCA TCATTCAGCA GCCGGCCAAC GGGGGCAGGG ACTGCACGGA CCCGCTCTAC
    GAAGAGAAAG TCTGTGAGGC CCCTCAAGTG TGCCAAAGCT ACAGGTGGAA GACCCACAAA
    TGGCGCAGGT GCCAGTTGGT CCCTTGGAGC GTGCGACAGG ACAGCCCTGG AGCACAGGAA
    GGCTGCGGAC CCGGACGCCA GGCAAGAGCT ATTACTTGTC GAAAGCAAGA TGGAGGACAG
    GCTGGCATCC ATGAATGCCT CTGGTATGCA GGCCCTGTGC CAGCCCTCAC CCAGGCCTGC
    CAGATCCCAT GCCAAGATGA CTGTCAGTTT ACCAGCTGGT CCAAGTTTTC TTCCTGCAAC
    GGAGACTGTG GTGCAGTTAG GACCAGAAAA CGCACTATTG TTGGAAAAAG TAAAAAGAAG
    GAAAAATGTA AAAATTCTCA TTTGTACCCC CTGATTGAGA CTCAGTACTG TCCTTGTGAC
    AAGTATAATG CACAGCCTGT GGGGAACTGG TCAGACTGCA TTTTACCAGA GGGGAAAGTG
    GAAGTGTTGC TTGGAGTGAA AACACAAGGA GATACCAAGG AATGTGGTCA AGGATATCGT
    TACCAAGCAA TGGCATGCTA CGACCAAAAT GGCAGGCTCG TGGAAACATC CAGATGTAAC
    AGTCATGGTT ACATTGAAGA GGCCTGCATC ATCCCTTGTC CTTCTGATTG CAAGCTCAGT
    GAGTGGTCCA ACTGGTCTCG CTGCAGTAAG TCCTGTGGAA GTGGTGTGAA GGTTCGGTCT
    AAATGGTTGC GTGAGAAACC ATATAATGGA GGGAGGCCTT GCCCCAAGCT GGACCATGTC
    AACCAGGCAC AGGTGTATGA GGTTGTCCCT TGCCACAGCG ACTGCAACCA GTACATATGG
    GTCACAGAGC CATGGAGCAT CTGCAAAGTG ACCTTTGTCA ACATGCGAGA TAACTGTGGA
    GAGGGTGTGC AAACCCGAAA AGTGAGATGC ATGCAGAACA CAGCAGATGG CCCTTCTGAC
    CATGTAGAAG ATTACCTCTG CGATCCAGAA GAGATGCCCC TGGGCTCTAG AGAGTGCAAA
    TTACCATGCC CTGAGGATTG TGTGATATCT GAATGGAGTC CATGGACCCG CTGCACTTTG
    CCTTGCAGTC AAAGCAGTTT CCGGCAAAGG TCAGCTGATC CCATCAGACA ACCTGCAGAT
    GAAGGAAGAG CCTGCCCTAA TGCTGTTGAG AAGGAACCCT GTAACCTAAA CAAAAACTGC
    TTCCACTATG ATTATAATGT AACAGACTGG AGTACGTGTC AGCTGAGTGA GAAGGCAGTT
    TGTGGAAACG GCATTAAAAC AAGGATGTTG GATTGTGTTC GAAGCGATGG CAAGTCGGTT
    GACCTGAAAT ATTGTGAAGA GCTTGGCCTG GAGAAGACCT GGCAGATGAA CACATCCTGC
    ATGGTAGAAT GTCCCGTCAA CTGCCAGCTT TCCGACTGGT CTCCTTGGTC AGAATGTTCT
    CAAACATGTG GCCTCACAGG GAAAATGATC CGAAGACGCA CAGTGACCCA GCCCTTTCAA
    GGTGATGGAC GGCCATGCCC TGCCCTGATG GAACAGTCCA AGCCTTGCCC AGTGAAGCCC
    TGTTATCAGT GGCAATATGG CCAGTGGTCT CTATGCCAAG TGCAGGATGC CCAGTGTGGC
    GAAGGGACGA GAACAAGGAA CATTTCTTGT GTAGTGAGTG ACGGGTCAAC TGATGATTTC
    AACAAAGTGG TGGATGAAGA ATTCTGTACT GACATTGAAC CCATAATAGA TGGTAATAAA
    AAAATGGTTC TCGAGGAAAC CTGCACCAAG CCTTGCCCAG GTGACTGTTA TCTGAAGGAC
    TGGTCTTCCT GGAGCCTGTG TCAGCTAACC TGTGTGAATG GTGAAGATCT AGGCTTTGGT
    GGAATACAGG TCCGTTCCAG AGCTGTGATT ATACAAGAGC TAGAAAATCA GCATCTGTGC
    CCAGAGCAAA TGTTAGAAAC AAAATCATGT AATGATGGAC AGTGTTATGA GTATAAATGG
    ATGGCCAGTG CTTGGAAGGG TTCTTCCCGA ACAGTCTGGT GTCAAAGGTC AGATGGTATA
    AATGTAACAG ATGGGAGACT AAAGACCTGG GTTTACGGTG TAGCAGCTGG GGCATTTGTG
    TTACTCATCT TTATTGTCTC CATGATTTAT CTAGCTTGCA AAAAGCCAAA GAAACCCCAA
    AGAAGGCAAA ACAACCGACT GAAACCTTTA ACCCTAGCCT ACGATGGAGA TGCAGACATG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe293243
    Clone ID Related Accession (Same CDS sequence) XM_022427413.1
    Accession Version XM_022427413.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4614bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product thrombospondin type-1 domain-containing protein 7A isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006596.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGGCTGC AAGCGAGGCG CTCGGCGTCC CAGAGCCCCG GCGCCCCGGG CCCGCGCCGG 
    GGTGTCCTGC AGCTGCTGCC GCTGCTCCTG CTGCTGCTGC TGCTGCTGCT GCTGCGCCCC
    GGAGCCTGCG GGGCTGCGGC TCCCGGCGGG GCTGAGGCAC CCACCCTCTA CCTCTGGAAG
    ACTGGTCCAT GGGGCCGGTG TATGGGAGAT GAATGTGGTC CAGGAGGCAT TCAGACCCGG
    GCCGTGTGGT GTGCTCATGT GGAAGGATGG ACCACATTAC ATACAAACTG TAAGCAGGCT
    GAGAGACCCA GCAACCAGCA AAACTGTTTC AAGGTGTGTG ACTGGCACAA GGAGTTGTAT
    GATTGGAGGC TGGGACCTTG GAACCAGTGT CAGCCTGTGA TATCCAAGAG CCTGGAGAAA
    CCCCCTGAGT GCATTAAGGG GGAGGAAGGC ATCCAGGTGA GAGAAATAAC GTGCATCCAG
    AAAGAGAAGG ACATTCCTGC AGAGGATATC ATTTGTGAGT ACTTTGAGCC CAAACCTCTC
    CTGGAACAGG CCTGCCTCAT CCCCTGCCAG CAAGATTGCA TCGTGTCTGA GTTTTCCGCG
    TGGTCAGAAT GTTCCAAGAC TTGTGGCAGT GGGCTCCAGC ACCGGACTCG GCATGTGGTG
    GCGCCTCCCC AGTTTGGAGG TTCTGGCTGC CCAAACTTGA CAGAGTTTCA GGTGTGCCAG
    TCCAGCCCCT GTGAAGATGA AGAGAGCATG TACAGCTTGC ACGTGGGTCC CTGGAGCTCA
    TGCTCGATGT CCCACTCAAG ACAAGTAAGA CAAGCAAGGA GACGTGGGAA GAATAAAGAA
    CGGGAGAAGG ACCGAGGAAA AGGAGTGAAG GATCCCGAAG CCCGTGAGCT TATTAAGAAA
    AAGAGAAACA GAAACAGACA GAATCGACAA GAGAACAAAT ATTGGGACAT CCAGATTGGG
    TATCAGACCA GAGAGGTTAT GTGTACTAAC AAGACGGGAA AAGCTGTTGA TTTGAGCTTT
    TGCCAGCAAG AGAAGCTGCC AATGACCTTC CAGTCCTGTG TGATCACCAA AGAGTGCCAG
    GTGTCAGAGT GGTCAGAATG GAGTCCCTGC TCAAAAACAT GCCATGACAT GACATCCCCC
    AGAGGCACTC GCGTCAGGAC GCGGATCATT AGGCAGTTTC CTATTGGCAG CGAGAAAGAG
    TGTCCAGAAC TTGAAGAAAA AGAACCCTGT GTGTCTCAAG GAGATGCAGT TGCCCCCTGT
    GCCACGTATG GCTGGAGAAC TACAGAGTGG GCGGAGTGCC GTGTGGACCC CTTGCTCAGT
    CAGCAGGACA AGAGGCGGGG CAACCAGACA GCCCTCTGTG GAGGAGGAGT CCAGACACGA
    GAGGTGTACT GCGTGCAGGC CAATGAAAAC CTCCTCTCAC ACTTAAACAC CCACAAGGAC
    AAAGAAGAAA GTAAATATCA GAGTACGTCG AAGTCTAGTG CTGCACCTTT CCTATCCATC
    ACTTTTGAAA TAACAGCCTC AAAACCAGTG GACTTGAAAT CATGCACTGG CCCTGTCCCT
    AATACTACAC AGCTGTGCCA CATTCCCTGT CCTATTGAAT GTGAGGTTTC ACCTTGGTCA
    GCTTGGGGAC CTTGTACTTA TGAAAACTGT AATGACCAGC AAGGCAAAAA AGGTTTCAAA
    CTGAGGAAGC GACGCATTAC CAATGAGCCC ACTGGAGGCT CTGGGGGCAC TGGGAACTGC
    CCTCACTTAC TGGAAGCCAT TCCCTGTGAA GAGCCATCCT GCTATGAATG GAAAGCAGTG
    AGGCTTGGAG ACTGTGAACC AGACAATGGA AAAGAGTGTG GCCCAGGCAC TCAAGTTCAG
    GAGGTTGTAT GCATCAACAG TGATGGAGAA GAAGTTGACA GACAGCTGTG CAGAGATGCC
    ATCTTCCCCA TCCCCGTTGC CTGTTATGCC CCATGCCCAA AGGACTGTGT GCTCAGCACG
    TGGTCTGCAT GGTCCTCCTG CTCACACACC TGCTCGGGGA AAACCACAGA AGGGAAACAG
    ATACGAGCAC GGTCCATTCT GGCCTATGCC GGTGACGAAG GTGGAGCTCA CTGTCCAAAT
    AGCAGTGCTT TGCAAGAGGT GCGCAGTTGT AACGAGCATC CCTGCACTGT GTACCACTGG
    CAGACTGGTC CCTGGGGCCA GTGCATAGAG GACACTTCAG TATCATCCTT CAACACGACG
    ACTACTTGGA ATGGAGAGGC GTCTTGCTCT GTTGGCATGC AGACAAGAAA AGTCATTTGT
    GTGCGGGTCA ATGTGGGCCA AGTAGGACCC AAAAAGTGTC CTGAAAGCCT TAGACCTGAA
    ACAGTGAGGC CCTGTCTGCT TCCTTGTAGA AAGGACTGTA TTGTGACACC ATATAGTGAC
    TGGACATCAT GCCCCAACTC ATGTAAAGAA GGGGACTCCG GAGTCAGAAA GCAGTCTCGG
    CACCGGGTCA TCATTCAGCA GCCGGCCAAC GGGGGCAGGG ACTGCACGGA CCCGCTCTAC
    GAAGAGAAAG TCTGTGAGGC CCCTCAAGTG TGCCAAAGCT ACAGGTGGAA GACCCACAAA
    TGGCGCAGGT GCCAGTTGGT CCCTTGGAGC GTGCGACAGG ACAGCCCTGG AGCACAGGAA
    GGCTGCGGAC CCGGACGCCA GGCAAGAGCT ATTACTTGTC GAAAGCAAGA TGGAGGACAG
    GCTGGCATCC ATGAATGCCT CTGGTATGCA GGCCCTGTGC CAGCCCTCAC CCAGGCCTGC
    CAGATCCCAT GCCAAGATGA CTGTCAGTTT ACCAGCTGGT CCAAGTTTTC TTCCTGCAAC
    GGAGACTGTG GTGCAGTTAG GACCAGAAAA CGCACTATTG TTGGAAAAAG TAAAAAGAAG
    GAAAAATGTA AAAATTCTCA TTTGTACCCC CTGATTGAGA CTCAGTACTG TCCTTGTGAC
    AAGTATAATG CACAGCCTGT GGGGAACTGG TCAGACTGCA TTTTACCAGA GGGGAAAGTG
    GAAGTGTTGC TTGGAGTGAA AACACAAGGA GATACCAAGG AATGTGGTCA AGGATATCGT
    TACCAAGCAA TGGCATGCTA CGACCAAAAT GGCAGGCTCG TGGAAACATC CAGATGTAAC
    AGTCATGGTT ACATTGAAGA GGCCTGCATC ATCCCTTGTC CTTCTGATTG CAAGCTCAGT
    GAGTGGTCCA ACTGGTCTCG CTGCAGTAAG TCCTGTGGAA GTGGTGTGAA GGTTCGGTCT
    AAATGGTTGC GTGAGAAACC ATATAATGGA GGGAGGCCTT GCCCCAAGCT GGACCATGTC
    AACCAGGCAC AGGTGTATGA GGTTGTCCCT TGCCACAGCG ACTGCAACCA GTACATATGG
    GTCACAGAGC CATGGAGCAT CTGCAAAGTG ACCTTTGTCA ACATGCGAGA TAACTGTGGA
    GAGGGTGTGC AAACCCGAAA AGTGAGATGC ATGCAGAACA CAGCAGATGG CCCTTCTGAC
    CATGTAGAAG ATTACCTCTG CGATCCAGAA GAGATGCCCC TGGGCTCTAG AGAGTGCAAA
    TTACCATGCC CTGAGGATTG TGTGATATCT GAATGGAGTC CATGGACCCG CTGCACTTTG
    CCTTGCAGTC AAAGCAGTTT CCGGCAAAGG TCAGCTGATC CCATCAGACA ACCTGCAGAT
    GAAGGAAGAG CCTGCCCTAA TGCTGTTGAG AAGGAACCCT GTAACCTAAA CAAAAACTGC
    TTCCACTATG ATTATAATGT AACAGACTGG AGTACGTGTC AGCTGAGTGA GAAGGCAGTT
    TGTGGAAACG GCATTAAAAC AAGGATGTTG GATTGTGTTC GAAGCGATGG CAAGTCGGTT
    GACCTGAAAT ATTGTGAAGA GCTTGGCCTG GAGAAGACCT GGCAGATGAA CACATCCTGC
    ATGGTAGAAT GTCCCGTCAA CTGCCAGCTT TCCGACTGGT CTCCTTGGTC AGAATGTTCT
    CAAACATGTG GCCTCACAGG GAAAATGATC CGAAGACGCA CAGTGACCCA GCCCTTTCAA
    GGTGATGGAC GGCCATGCCC TGCCCTGATG GAACAGTCCA AGCCTTGCCC AGTGAAGCCC
    TGTTATCAGT GGCAATATGG CCAGTGGTCT CTATGCCAAG TGCAGGATGC CCAGTGTGGC
    GAAGGGACGA GAACAAGGAA CATTTCTTGT GTAGTGAGTG ACGGGTCAAC TGATGATTTC
    AACAAAGTGG TGGATGAAGA ATTCTGTACT GACATTGAAC CCATAATAGA TGGTAATAAA
    AAAATGGTTC TCGAGGAAAC CTGCACCAAG CCTTGCCCAG GTGACTGTTA TCTGAAGGAC
    TGGTCTTCCT GGAGCCTGTG TCAGCTAACC TGTGTGAATG GTGAAGATCT AGGCTTTGGT
    GGAATACAGG TCCGTTCCAG AGCTGTGATT ATACAAGAGC TAGAAAATCA GCATCTGTGC
    CCAGAGCAAA TGTTAGAAAC AAAATCATGT AATGATGGAC AGTGTTATGA GTATAAATGG
    ATGGCCAGTG CTTGGAAGGG TTCTTCCCGA ACAGTCTGGT GTCAAAGGTC AGATGGTATA
    AATGTAACAG CAAAAAGCCA AAGAAACCCC AAAGAAGGCA AAACAACCGA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022283121.1
    CDS270..4883
    Translation

    Target ORF information:

    RefSeq Version XM_022427413.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris thrombospondin type 1 domain containing 7A (THSD7A), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022427413.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGGCTGC AAGCGAGGCG CTCGGCGTCC CAGAGCCCCG GCGCCCCGGG CCCGCGCCGG 
    GGTGTCCTGC AGCTGCTGCC GCTGCTCCTG CTGCTGCTGC TGCTGCTGCT GCTGCGCCCC
    GGAGCCTGCG GGGCTGCGGC TCCCGGCGGG GCTGAGGCAC CCACCCTCTA CCTCTGGAAG
    ACTGGTCCAT GGGGCCGGTG TATGGGAGAT GAATGTGGTC CAGGAGGCAT TCAGACCCGG
    GCCGTGTGGT GTGCTCATGT GGAAGGATGG ACCACATTAC ATACAAACTG TAAGCAGGCT
    GAGAGACCCA GCAACCAGCA AAACTGTTTC AAGGTGTGTG ACTGGCACAA GGAGTTGTAT
    GATTGGAGGC TGGGACCTTG GAACCAGTGT CAGCCTGTGA TATCCAAGAG CCTGGAGAAA
    CCCCCTGAGT GCATTAAGGG GGAGGAAGGC ATCCAGGTGA GAGAAATAAC GTGCATCCAG
    AAAGAGAAGG ACATTCCTGC AGAGGATATC ATTTGTGAGT ACTTTGAGCC CAAACCTCTC
    CTGGAACAGG CCTGCCTCAT CCCCTGCCAG CAAGATTGCA TCGTGTCTGA GTTTTCCGCG
    TGGTCAGAAT GTTCCAAGAC TTGTGGCAGT GGGCTCCAGC ACCGGACTCG GCATGTGGTG
    GCGCCTCCCC AGTTTGGAGG TTCTGGCTGC CCAAACTTGA CAGAGTTTCA GGTGTGCCAG
    TCCAGCCCCT GTGAAGATGA AGAGAGCATG TACAGCTTGC ACGTGGGTCC CTGGAGCTCA
    TGCTCGATGT CCCACTCAAG ACAAGTAAGA CAAGCAAGGA GACGTGGGAA GAATAAAGAA
    CGGGAGAAGG ACCGAGGAAA AGGAGTGAAG GATCCCGAAG CCCGTGAGCT TATTAAGAAA
    AAGAGAAACA GAAACAGACA GAATCGACAA GAGAACAAAT ATTGGGACAT CCAGATTGGG
    TATCAGACCA GAGAGGTTAT GTGTACTAAC AAGACGGGAA AAGCTGTTGA TTTGAGCTTT
    TGCCAGCAAG AGAAGCTGCC AATGACCTTC CAGTCCTGTG TGATCACCAA AGAGTGCCAG
    GTGTCAGAGT GGTCAGAATG GAGTCCCTGC TCAAAAACAT GCCATGACAT GACATCCCCC
    AGAGGCACTC GCGTCAGGAC GCGGATCATT AGGCAGTTTC CTATTGGCAG CGAGAAAGAG
    TGTCCAGAAC TTGAAGAAAA AGAACCCTGT GTGTCTCAAG GAGATGCAGT TGCCCCCTGT
    GCCACGTATG GCTGGAGAAC TACAGAGTGG GCGGAGTGCC GTGTGGACCC CTTGCTCAGT
    CAGCAGGACA AGAGGCGGGG CAACCAGACA GCCCTCTGTG GAGGAGGAGT CCAGACACGA
    GAGGTGTACT GCGTGCAGGC CAATGAAAAC CTCCTCTCAC ACTTAAACAC CCACAAGGAC
    AAAGAAGAAA GTAAATATCA GAGTACGTCG AAGTCTAGTG CTGCACCTTT CCTATCCATC
    ACTTTTGAAA TAACAGCCTC AAAACCAGTG GACTTGAAAT CATGCACTGG CCCTGTCCCT
    AATACTACAC AGCTGTGCCA CATTCCCTGT CCTATTGAAT GTGAGGTTTC ACCTTGGTCA
    GCTTGGGGAC CTTGTACTTA TGAAAACTGT AATGACCAGC AAGGCAAAAA AGGTTTCAAA
    CTGAGGAAGC GACGCATTAC CAATGAGCCC ACTGGAGGCT CTGGGGGCAC TGGGAACTGC
    CCTCACTTAC TGGAAGCCAT TCCCTGTGAA GAGCCATCCT GCTATGAATG GAAAGCAGTG
    AGGCTTGGAG ACTGTGAACC AGACAATGGA AAAGAGTGTG GCCCAGGCAC TCAAGTTCAG
    GAGGTTGTAT GCATCAACAG TGATGGAGAA GAAGTTGACA GACAGCTGTG CAGAGATGCC
    ATCTTCCCCA TCCCCGTTGC CTGTTATGCC CCATGCCCAA AGGACTGTGT GCTCAGCACG
    TGGTCTGCAT GGTCCTCCTG CTCACACACC TGCTCGGGGA AAACCACAGA AGGGAAACAG
    ATACGAGCAC GGTCCATTCT GGCCTATGCC GGTGACGAAG GTGGAGCTCA CTGTCCAAAT
    AGCAGTGCTT TGCAAGAGGT GCGCAGTTGT AACGAGCATC CCTGCACTGT GTACCACTGG
    CAGACTGGTC CCTGGGGCCA GTGCATAGAG GACACTTCAG TATCATCCTT CAACACGACG
    ACTACTTGGA ATGGAGAGGC GTCTTGCTCT GTTGGCATGC AGACAAGAAA AGTCATTTGT
    GTGCGGGTCA ATGTGGGCCA AGTAGGACCC AAAAAGTGTC CTGAAAGCCT TAGACCTGAA
    ACAGTGAGGC CCTGTCTGCT TCCTTGTAGA AAGGACTGTA TTGTGACACC ATATAGTGAC
    TGGACATCAT GCCCCAACTC ATGTAAAGAA GGGGACTCCG GAGTCAGAAA GCAGTCTCGG
    CACCGGGTCA TCATTCAGCA GCCGGCCAAC GGGGGCAGGG ACTGCACGGA CCCGCTCTAC
    GAAGAGAAAG TCTGTGAGGC CCCTCAAGTG TGCCAAAGCT ACAGGTGGAA GACCCACAAA
    TGGCGCAGGT GCCAGTTGGT CCCTTGGAGC GTGCGACAGG ACAGCCCTGG AGCACAGGAA
    GGCTGCGGAC CCGGACGCCA GGCAAGAGCT ATTACTTGTC GAAAGCAAGA TGGAGGACAG
    GCTGGCATCC ATGAATGCCT CTGGTATGCA GGCCCTGTGC CAGCCCTCAC CCAGGCCTGC
    CAGATCCCAT GCCAAGATGA CTGTCAGTTT ACCAGCTGGT CCAAGTTTTC TTCCTGCAAC
    GGAGACTGTG GTGCAGTTAG GACCAGAAAA CGCACTATTG TTGGAAAAAG TAAAAAGAAG
    GAAAAATGTA AAAATTCTCA TTTGTACCCC CTGATTGAGA CTCAGTACTG TCCTTGTGAC
    AAGTATAATG CACAGCCTGT GGGGAACTGG TCAGACTGCA TTTTACCAGA GGGGAAAGTG
    GAAGTGTTGC TTGGAGTGAA AACACAAGGA GATACCAAGG AATGTGGTCA AGGATATCGT
    TACCAAGCAA TGGCATGCTA CGACCAAAAT GGCAGGCTCG TGGAAACATC CAGATGTAAC
    AGTCATGGTT ACATTGAAGA GGCCTGCATC ATCCCTTGTC CTTCTGATTG CAAGCTCAGT
    GAGTGGTCCA ACTGGTCTCG CTGCAGTAAG TCCTGTGGAA GTGGTGTGAA GGTTCGGTCT
    AAATGGTTGC GTGAGAAACC ATATAATGGA GGGAGGCCTT GCCCCAAGCT GGACCATGTC
    AACCAGGCAC AGGTGTATGA GGTTGTCCCT TGCCACAGCG ACTGCAACCA GTACATATGG
    GTCACAGAGC CATGGAGCAT CTGCAAAGTG ACCTTTGTCA ACATGCGAGA TAACTGTGGA
    GAGGGTGTGC AAACCCGAAA AGTGAGATGC ATGCAGAACA CAGCAGATGG CCCTTCTGAC
    CATGTAGAAG ATTACCTCTG CGATCCAGAA GAGATGCCCC TGGGCTCTAG AGAGTGCAAA
    TTACCATGCC CTGAGGATTG TGTGATATCT GAATGGAGTC CATGGACCCG CTGCACTTTG
    CCTTGCAGTC AAAGCAGTTT CCGGCAAAGG TCAGCTGATC CCATCAGACA ACCTGCAGAT
    GAAGGAAGAG CCTGCCCTAA TGCTGTTGAG AAGGAACCCT GTAACCTAAA CAAAAACTGC
    TTCCACTATG ATTATAATGT AACAGACTGG AGTACGTGTC AGCTGAGTGA GAAGGCAGTT
    TGTGGAAACG GCATTAAAAC AAGGATGTTG GATTGTGTTC GAAGCGATGG CAAGTCGGTT
    GACCTGAAAT ATTGTGAAGA GCTTGGCCTG GAGAAGACCT GGCAGATGAA CACATCCTGC
    ATGGTAGAAT GTCCCGTCAA CTGCCAGCTT TCCGACTGGT CTCCTTGGTC AGAATGTTCT
    CAAACATGTG GCCTCACAGG GAAAATGATC CGAAGACGCA CAGTGACCCA GCCCTTTCAA
    GGTGATGGAC GGCCATGCCC TGCCCTGATG GAACAGTCCA AGCCTTGCCC AGTGAAGCCC
    TGTTATCAGT GGCAATATGG CCAGTGGTCT CTATGCCAAG TGCAGGATGC CCAGTGTGGC
    GAAGGGACGA GAACAAGGAA CATTTCTTGT GTAGTGAGTG ACGGGTCAAC TGATGATTTC
    AACAAAGTGG TGGATGAAGA ATTCTGTACT GACATTGAAC CCATAATAGA TGGTAATAAA
    AAAATGGTTC TCGAGGAAAC CTGCACCAAG CCTTGCCCAG GTGACTGTTA TCTGAAGGAC
    TGGTCTTCCT GGAGCCTGTG TCAGCTAACC TGTGTGAATG GTGAAGATCT AGGCTTTGGT
    GGAATACAGG TCCGTTCCAG AGCTGTGATT ATACAAGAGC TAGAAAATCA GCATCTGTGC
    CCAGAGCAAA TGTTAGAAAC AAAATCATGT AATGATGGAC AGTGTTATGA GTATAAATGG
    ATGGCCAGTG CTTGGAAGGG TTCTTCCCGA ACAGTCTGGT GTCAAAGGTC AGATGGTATA
    AATGTAACAG CAAAAAGCCA AAGAAACCCC AAAGAAGGCA AAACAACCGA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.