TSC1 cDNA ORF clone, Pan troglodytes(chimpanzee)

The following TSC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TSC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OPc89759 XM_016961952.2
Latest version!
Pan troglodytes TSC complex subunit 1 (TSC1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OPc89759 XM_016961954.2
Latest version!
Pan troglodytes TSC complex subunit 1 (TSC1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OPc89759 XM_016961953.2
Latest version!
Pan troglodytes TSC complex subunit 1 (TSC1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OPc13015 XM_016961955.2
Latest version!
Pan troglodytes TSC complex subunit 1 (TSC1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OPc89759 XM_016961953.1 Pan troglodytes tuberous sclerosis 1 (TSC1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OPc89759 XM_016961954.1 Pan troglodytes tuberous sclerosis 1 (TSC1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OPc89759 XM_016961952.1 Pan troglodytes tuberous sclerosis 1 (TSC1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OPc13015 XM_016961955.1 Pan troglodytes tuberous sclerosis 1 (TSC1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OPc89759
    Clone ID Related Accession (Same CDS sequence) XM_016961954.1 , XM_016961953.1 , XM_016961953.2 , XM_016961952.2 , XM_016961954.2 , XM_016961952.1
    Accession Version XM_016961954.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product hamartin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006476.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGTCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAACT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
    GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
    AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
    CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
    CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
    CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
    TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
    AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
    ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
    CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GACGTTGTTA
    AATACGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
    CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
    TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
    GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
    TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
    AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
    GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
    GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
    GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
    GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
    TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
    GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
    AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
    TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
    CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
    ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
    CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCACAC
    AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
    GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
    CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
    CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
    AAGTTACAAG AGAAGGACAT CCAGATGTGG AAAGTTAGTC TGCAGAAAGA ACAAGCTAGA
    TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
    CAGCTGCAGC ACGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
    GAGGACTGCA GGAACATGAT TGCAGAGCTG CGGATAGAAT TGAAGAAGGC CAACAACAAG
    GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
    GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
    CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
    AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
    AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
    CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
    CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
    GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAG
    GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
    TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
    CCCAGGCCCA GCAGCACCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
    AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
    AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
    CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
    GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
    CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
    TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
    CATGAACACA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016817443.1
    CDS332..3826
    Translation

    Target ORF information:

    RefSeq Version XM_016961954.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes tuberous sclerosis 1 (TSC1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016961954.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGTCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAACT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
    GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
    AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
    CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
    CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
    CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
    TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
    AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
    ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
    CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GACGTTGTTA
    AATACGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
    CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
    TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
    GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
    TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
    AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
    GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
    GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
    GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
    GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
    TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
    GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
    AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
    TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
    CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
    ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
    CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCACAC
    AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
    GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
    CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
    CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
    AAGTTACAAG AGAAGGACAT CCAGATGTGG AAAGTTAGTC TGCAGAAAGA ACAAGCTAGA
    TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
    CAGCTGCAGC ACGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
    GAGGACTGCA GGAACATGAT TGCAGAGCTG CGGATAGAAT TGAAGAAGGC CAACAACAAG
    GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
    GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
    CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
    AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
    AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
    CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
    CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
    GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAG
    GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
    TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
    CCCAGGCCCA GCAGCACCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
    AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
    AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
    CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
    GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
    CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
    TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
    CATGAACACA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc89759
    Clone ID Related Accession (Same CDS sequence) XM_016961954.1 , XM_016961953.1 , XM_016961953.2 , XM_016961952.2 , XM_016961954.2 , XM_016961952.1
    Accession Version XM_016961953.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product hamartin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006476.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGTCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAACT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
    GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
    AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
    CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
    CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
    CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
    TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
    AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
    ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
    CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GACGTTGTTA
    AATACGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
    CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
    TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
    GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
    TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
    AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
    GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
    GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
    GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
    GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
    TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
    GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
    AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
    TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
    CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
    ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
    CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCACAC
    AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
    GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
    CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
    CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
    AAGTTACAAG AGAAGGACAT CCAGATGTGG AAAGTTAGTC TGCAGAAAGA ACAAGCTAGA
    TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
    CAGCTGCAGC ACGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
    GAGGACTGCA GGAACATGAT TGCAGAGCTG CGGATAGAAT TGAAGAAGGC CAACAACAAG
    GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
    GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
    CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
    AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
    AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
    CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
    CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
    GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAG
    GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
    TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
    CCCAGGCCCA GCAGCACCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
    AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
    AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
    CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
    GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
    CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
    TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
    CATGAACACA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016817442.1
    CDS378..3872
    Translation

    Target ORF information:

    RefSeq Version XM_016961953.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes tuberous sclerosis 1 (TSC1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016961953.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGTCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAACT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
    GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
    AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
    CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
    CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
    CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
    TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
    AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
    ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
    CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GACGTTGTTA
    AATACGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
    CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
    TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
    GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
    TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
    AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
    GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
    GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
    GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
    GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
    TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
    GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
    AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
    TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
    CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
    ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
    CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCACAC
    AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
    GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
    CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
    CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
    AAGTTACAAG AGAAGGACAT CCAGATGTGG AAAGTTAGTC TGCAGAAAGA ACAAGCTAGA
    TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
    CAGCTGCAGC ACGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
    GAGGACTGCA GGAACATGAT TGCAGAGCTG CGGATAGAAT TGAAGAAGGC CAACAACAAG
    GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
    GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
    CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
    AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
    AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
    CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
    CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
    GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAG
    GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
    TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
    CCCAGGCCCA GCAGCACCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
    AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
    AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
    CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
    GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
    CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
    TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
    CATGAACACA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc89759
    Clone ID Related Accession (Same CDS sequence) XM_016961954.1 , XM_016961953.1 , XM_016961953.2 , XM_016961952.2 , XM_016961954.2 , XM_016961952.1
    Accession Version XM_016961953.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product hamartin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036888.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016961953.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGTCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAACT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
    GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
    AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
    CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
    CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
    CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
    TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
    AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
    ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
    CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GACGTTGTTA
    AATACGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
    CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
    TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
    GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
    TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
    AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
    GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
    GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
    GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
    GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
    TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
    GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
    AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
    TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
    CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
    ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
    CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCACAC
    AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
    GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
    CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
    CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
    AAGTTACAAG AGAAGGACAT CCAGATGTGG AAAGTTAGTC TGCAGAAAGA ACAAGCTAGA
    TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
    CAGCTGCAGC ACGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
    GAGGACTGCA GGAACATGAT TGCAGAGCTG CGGATAGAAT TGAAGAAGGC CAACAACAAG
    GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
    GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
    CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
    AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
    AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
    CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
    CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
    GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAG
    GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
    TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
    CCCAGGCCCA GCAGCACCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
    AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
    AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
    CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
    GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
    CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
    TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
    CATGAACACA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016817442.1
    CDS372..3866
    Translation

    Target ORF information:

    RefSeq Version XM_016961953.2
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes TSC complex subunit 1 (TSC1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016961953.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGTCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAACT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
    GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
    AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
    CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
    CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
    CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
    TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
    AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
    ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
    CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GACGTTGTTA
    AATACGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
    CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
    TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
    GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
    TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
    AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
    GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
    GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
    GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
    GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
    TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
    GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
    AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
    TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
    CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
    ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
    CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCACAC
    AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
    GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
    CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
    CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
    AAGTTACAAG AGAAGGACAT CCAGATGTGG AAAGTTAGTC TGCAGAAAGA ACAAGCTAGA
    TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
    CAGCTGCAGC ACGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
    GAGGACTGCA GGAACATGAT TGCAGAGCTG CGGATAGAAT TGAAGAAGGC CAACAACAAG
    GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
    GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
    CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
    AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
    AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
    CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
    CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
    GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAG
    GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
    TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
    CCCAGGCCCA GCAGCACCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
    AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
    AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
    CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
    GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
    CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
    TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
    CATGAACACA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc89759
    Clone ID Related Accession (Same CDS sequence) XM_016961954.1 , XM_016961953.1 , XM_016961953.2 , XM_016961952.2 , XM_016961954.2 , XM_016961952.1
    Accession Version XM_016961952.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product hamartin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036888.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016961952.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGTCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAACT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
    GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
    AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
    CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
    CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
    CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
    TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
    AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
    ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
    CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GACGTTGTTA
    AATACGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
    CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
    TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
    GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
    TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
    AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
    GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
    GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
    GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
    GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
    TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
    GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
    AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
    TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
    CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
    ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
    CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCACAC
    AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
    GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
    CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
    CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
    AAGTTACAAG AGAAGGACAT CCAGATGTGG AAAGTTAGTC TGCAGAAAGA ACAAGCTAGA
    TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
    CAGCTGCAGC ACGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
    GAGGACTGCA GGAACATGAT TGCAGAGCTG CGGATAGAAT TGAAGAAGGC CAACAACAAG
    GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
    GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
    CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
    AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
    AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
    CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
    CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
    GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAG
    GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
    TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
    CCCAGGCCCA GCAGCACCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
    AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
    AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
    CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
    GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
    CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
    TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
    CATGAACACA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016817441.1
    CDS401..3895
    Translation

    Target ORF information:

    RefSeq Version XM_016961952.2
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes TSC complex subunit 1 (TSC1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016961952.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGTCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAACT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
    GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
    AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
    CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
    CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
    CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
    TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
    AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
    ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
    CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GACGTTGTTA
    AATACGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
    CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
    TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
    GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
    TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
    AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
    GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
    GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
    GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
    GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
    TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
    GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
    AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
    TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
    CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
    ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
    CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCACAC
    AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
    GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
    CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
    CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
    AAGTTACAAG AGAAGGACAT CCAGATGTGG AAAGTTAGTC TGCAGAAAGA ACAAGCTAGA
    TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
    CAGCTGCAGC ACGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
    GAGGACTGCA GGAACATGAT TGCAGAGCTG CGGATAGAAT TGAAGAAGGC CAACAACAAG
    GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
    GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
    CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
    AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
    AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
    CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
    CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
    GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAG
    GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
    TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
    CCCAGGCCCA GCAGCACCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
    AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
    AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
    CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
    GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
    CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
    TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
    CATGAACACA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc89759
    Clone ID Related Accession (Same CDS sequence) XM_016961954.1 , XM_016961953.1 , XM_016961953.2 , XM_016961952.2 , XM_016961954.2 , XM_016961952.1
    Accession Version XM_016961954.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product hamartin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036888.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016961954.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGTCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAACT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
    GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
    AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
    CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
    CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
    CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
    TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
    AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
    ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
    CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GACGTTGTTA
    AATACGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
    CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
    TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
    GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
    TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
    AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
    GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
    GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
    GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
    GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
    TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
    GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
    AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
    TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
    CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
    ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
    CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCACAC
    AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
    GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
    CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
    CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
    AAGTTACAAG AGAAGGACAT CCAGATGTGG AAAGTTAGTC TGCAGAAAGA ACAAGCTAGA
    TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
    CAGCTGCAGC ACGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
    GAGGACTGCA GGAACATGAT TGCAGAGCTG CGGATAGAAT TGAAGAAGGC CAACAACAAG
    GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
    GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
    CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
    AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
    AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
    CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
    CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
    GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAG
    GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
    TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
    CCCAGGCCCA GCAGCACCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
    AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
    AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
    CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
    GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
    CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
    TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
    CATGAACACA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016817443.1
    CDS331..3825
    Translation

    Target ORF information:

    RefSeq Version XM_016961954.2
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes TSC complex subunit 1 (TSC1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016961954.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGTCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAACT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
    GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
    AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
    CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
    CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
    CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
    TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
    AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
    ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
    CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GACGTTGTTA
    AATACGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
    CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
    TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
    GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
    TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
    AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
    GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
    GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
    GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
    GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
    TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
    GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
    AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
    TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
    CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
    ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
    CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCACAC
    AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
    GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
    CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
    CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
    AAGTTACAAG AGAAGGACAT CCAGATGTGG AAAGTTAGTC TGCAGAAAGA ACAAGCTAGA
    TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
    CAGCTGCAGC ACGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
    GAGGACTGCA GGAACATGAT TGCAGAGCTG CGGATAGAAT TGAAGAAGGC CAACAACAAG
    GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
    GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
    CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
    AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
    AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
    CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
    CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
    GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAG
    GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
    TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
    CCCAGGCCCA GCAGCACCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
    AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
    AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
    CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
    GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
    CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
    TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
    CATGAACACA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc89759
    Clone ID Related Accession (Same CDS sequence) XM_016961954.1 , XM_016961953.1 , XM_016961953.2 , XM_016961952.2 , XM_016961954.2 , XM_016961952.1
    Accession Version XM_016961952.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product hamartin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006476.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGTCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAACT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
    GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
    AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
    CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
    CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
    CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
    TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
    AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
    ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
    CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GACGTTGTTA
    AATACGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
    CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
    TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
    GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
    TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
    AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
    GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
    GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
    GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
    GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
    TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
    GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
    AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
    TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
    CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
    ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
    CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCACAC
    AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
    GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
    CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
    CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
    AAGTTACAAG AGAAGGACAT CCAGATGTGG AAAGTTAGTC TGCAGAAAGA ACAAGCTAGA
    TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
    CAGCTGCAGC ACGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
    GAGGACTGCA GGAACATGAT TGCAGAGCTG CGGATAGAAT TGAAGAAGGC CAACAACAAG
    GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
    GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
    CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
    AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
    AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
    CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
    CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
    GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAG
    GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
    TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
    CCCAGGCCCA GCAGCACCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
    AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
    AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
    CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
    GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
    CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
    TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
    CATGAACACA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016817441.1
    CDS407..3901
    Translation

    Target ORF information:

    RefSeq Version XM_016961952.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes tuberous sclerosis 1 (TSC1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016961952.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGTCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAACT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
    GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
    AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
    CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
    CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
    CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
    TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
    AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
    ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
    CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GACGTTGTTA
    AATACGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
    CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
    TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
    GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
    TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
    AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
    GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
    GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
    GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
    GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
    TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
    GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
    AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
    TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
    CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
    ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
    CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCACAC
    AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
    GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
    CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
    CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
    AAGTTACAAG AGAAGGACAT CCAGATGTGG AAAGTTAGTC TGCAGAAAGA ACAAGCTAGA
    TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
    CAGCTGCAGC ACGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
    GAGGACTGCA GGAACATGAT TGCAGAGCTG CGGATAGAAT TGAAGAAGGC CAACAACAAG
    GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
    GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
    CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
    AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
    AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
    CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
    CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
    GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAG
    GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
    TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
    CCCAGGCCCA GCAGCACCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
    AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
    AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
    CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
    GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
    CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
    TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
    CATGAACACA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc13015
    Clone ID Related Accession (Same CDS sequence) XM_016961955.2 , XM_016961955.1
    Accession Version XM_016961955.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3492bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product hamartin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036888.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016961955.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGTCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAACT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
    GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
    AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
    CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
    CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
    CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
    TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
    AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
    ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
    CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GACGTTGTTA
    AATACGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
    CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
    TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
    GGTGGAAAAG GAACTCCTCT GGGAACCCCA GCAACCTCTC CTCCTCCAGC CCCACTCTGT
    CATTCGGATG ACTACGTGCA CATTTCACTC CCCCAGGCCA CAGTCACACC CCCCAGGAAG
    GAAGAGAGAA TGGATTCTGC AAGACCATGT CTACACAGAC AACACCATCT TCTGAATGAC
    AGAGGATCAG AAGAGCCACC TGGCAGCAAA GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG
    TTTTTAGGTG ATCTGGCCTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCTGCA
    ATATCTAGAG AACTTTCTGA GATCACCACA GCAGAGGCAG AGCCTGTGGT TCCTCGAGGA
    GGCTTTGACT CTCCCTTTTA CCGAGACAGT CTCCCAGGTT CTCAGCGGAA GACCCACTCG
    GCAGCCTCCA GTTCTCAGGG CGCCAGCGTG AACCCTGAGC CTTTACACTC CTCCCTGGAC
    AAGCTTGGGC CTGACACACC AAAGCAAGCC TTTACTCCCA TAGACCTGCC CTGCGGCAGT
    GCTGATGAAA GCCCTGCGGG AGACAGGGAA TGCCAGACTT CTTTGGAGAC CAGTATCTTC
    ACTCCCAGTC CTTGTAAAAT TCCACCTCCG ACGAGAGTGG GCTTTGGAAG CGGGCAGCCT
    CCCCCGTATG ATCATCTTTT TGAGGTGGCA TTGCCAAAGA CAGCCCATCA TTTTGTCATC
    AGGAAGACTG AGGAGCTGTT AAAGAAAGCA AAAGGAAACA CAGAGGAAGA TGGTGTGCCC
    TCTACCTCCC CAATGGAAGT GCTGGACAGA CTGATACAGC AGGGAGCAGA CGCACACAGC
    AAGGAGCTGA ACAAGTTGCC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTTGGAGGC
    TCTCCTCCTT CAGATGAGAT CCGCACCCTC CGAGACCAGT TGCTTTTACT GCACAACCAG
    TTACTCTATG AGCGTTTTAA GAGGCAGCAG CATGCCCTCC GGAACAGGCG GCTCCTCCGC
    AAGGTGATCA AAGCAGCAGC TCTGGAGGAA CATAATGCTG CCATGAAAGA TCAGTTGAAG
    TTACAAGAGA AGGACATCCA GATGTGGAAA GTTAGTCTGC AGAAAGAACA AGCTAGATAC
    AATCAGCTCC AGGAGCAGCG TGACACTATG GTAACCAAGC TCCACAGCCA GATCAGACAG
    CTGCAGCACG ACCGAGAGGA ATTCTACAAC CAGAGCCAGG AATTACAGAC GAAGCTGGAG
    GACTGCAGGA ACATGATTGC AGAGCTGCGG ATAGAATTGA AGAAGGCCAA CAACAAGGTG
    TGTCACACTG AGCTGCTGCT CAGTCAGGTT TCCCAAAAGC TCTCAAACAG TGAGTCGGTC
    CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAGGT CAACGAGCTC
    TATTTGGAAC AACTGCAGAA CAAGCACTCA GATACCACAA AGGAAGTAGA AATGATGAAA
    GCCGCCTATC GGAAAGAGCT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GACTCAGAGG
    CTTGATACCT CCCAAAAACG GATTTTGGAA CTGGAATCTC ACCTGGCCAA GAAAGACCAC
    CTTCTTTTGG AACAGAAGAA ATATCTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG
    CAGGCCGCAG AGAGCAGGTA TGAGGCTCAG AAAAGGATAA CCCAGGTGTT TGAATTGGAG
    ATCTTAGATT TATATGGCAG GTTGGAGAAA GATGGCCTCC TGAAAAAACT TGAAGAGGAA
    AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA AGGCTTGACT GTTGTAATGA CGGGTGCTCA
    GATTCCATGG TAGGGCACAA TGAAGAGGCA TCTGGCCACA ACGGTGAGAC CAAGACCCCC
    AGGCCCAGCA GCACCCGGGG CAGTAGTGGA AGCAGAGGTG GTGGAGGCAG CAGCAGCAGC
    AGCAGCGAGC TTTCTACCCC AGAGAAACCC CCACACCAGA GGGCAGGCCC ATTCAGCAGT
    CGGTGGGAGA CGACTATGGG AGAAGCGTCT GCCAGCATCC CCACCACTGT GGGCTCACTT
    CCCAGTTCAA AAAGCTTCCT GGGTATGAAG GCTCGAGAGT TATTTCGTAA TAAGAGCGAG
    AGCCAGTGTG ATGAGGACGG CATGACCAGT AGCCTTTCTG AGAGCCTAAA GACAGAACTG
    GGCAAAGACT TGGGTGTGGA AGCCAAGATT CCCCTGAACC TAGATGGCCC TCACCCGTCT
    CCCCCGACCC CGGACAGTGT TGGACAGCTA CATATCATGG ACTACAATGA GACTCATCAT
    GAACACAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016817444.1
    CDS402..3893
    Translation

    Target ORF information:

    RefSeq Version XM_016961955.2
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes TSC complex subunit 1 (TSC1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016961955.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGTCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAACT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
    GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
    AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
    CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
    CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
    CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
    TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
    AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
    ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
    CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GACGTTGTTA
    AATACGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
    CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
    TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
    GGTGGAAAAG GAACTCCTCT GGGAACCCCA GCAACCTCTC CTCCTCCAGC CCCACTCTGT
    CATTCGGATG ACTACGTGCA CATTTCACTC CCCCAGGCCA CAGTCACACC CCCCAGGAAG
    GAAGAGAGAA TGGATTCTGC AAGACCATGT CTACACAGAC AACACCATCT TCTGAATGAC
    AGAGGATCAG AAGAGCCACC TGGCAGCAAA GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG
    TTTTTAGGTG ATCTGGCCTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCTGCA
    ATATCTAGAG AACTTTCTGA GATCACCACA GCAGAGGCAG AGCCTGTGGT TCCTCGAGGA
    GGCTTTGACT CTCCCTTTTA CCGAGACAGT CTCCCAGGTT CTCAGCGGAA GACCCACTCG
    GCAGCCTCCA GTTCTCAGGG CGCCAGCGTG AACCCTGAGC CTTTACACTC CTCCCTGGAC
    AAGCTTGGGC CTGACACACC AAAGCAAGCC TTTACTCCCA TAGACCTGCC CTGCGGCAGT
    GCTGATGAAA GCCCTGCGGG AGACAGGGAA TGCCAGACTT CTTTGGAGAC CAGTATCTTC
    ACTCCCAGTC CTTGTAAAAT TCCACCTCCG ACGAGAGTGG GCTTTGGAAG CGGGCAGCCT
    CCCCCGTATG ATCATCTTTT TGAGGTGGCA TTGCCAAAGA CAGCCCATCA TTTTGTCATC
    AGGAAGACTG AGGAGCTGTT AAAGAAAGCA AAAGGAAACA CAGAGGAAGA TGGTGTGCCC
    TCTACCTCCC CAATGGAAGT GCTGGACAGA CTGATACAGC AGGGAGCAGA CGCACACAGC
    AAGGAGCTGA ACAAGTTGCC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTTGGAGGC
    TCTCCTCCTT CAGATGAGAT CCGCACCCTC CGAGACCAGT TGCTTTTACT GCACAACCAG
    TTACTCTATG AGCGTTTTAA GAGGCAGCAG CATGCCCTCC GGAACAGGCG GCTCCTCCGC
    AAGGTGATCA AAGCAGCAGC TCTGGAGGAA CATAATGCTG CCATGAAAGA TCAGTTGAAG
    TTACAAGAGA AGGACATCCA GATGTGGAAA GTTAGTCTGC AGAAAGAACA AGCTAGATAC
    AATCAGCTCC AGGAGCAGCG TGACACTATG GTAACCAAGC TCCACAGCCA GATCAGACAG
    CTGCAGCACG ACCGAGAGGA ATTCTACAAC CAGAGCCAGG AATTACAGAC GAAGCTGGAG
    GACTGCAGGA ACATGATTGC AGAGCTGCGG ATAGAATTGA AGAAGGCCAA CAACAAGGTG
    TGTCACACTG AGCTGCTGCT CAGTCAGGTT TCCCAAAAGC TCTCAAACAG TGAGTCGGTC
    CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAGGT CAACGAGCTC
    TATTTGGAAC AACTGCAGAA CAAGCACTCA GATACCACAA AGGAAGTAGA AATGATGAAA
    GCCGCCTATC GGAAAGAGCT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GACTCAGAGG
    CTTGATACCT CCCAAAAACG GATTTTGGAA CTGGAATCTC ACCTGGCCAA GAAAGACCAC
    CTTCTTTTGG AACAGAAGAA ATATCTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG
    CAGGCCGCAG AGAGCAGGTA TGAGGCTCAG AAAAGGATAA CCCAGGTGTT TGAATTGGAG
    ATCTTAGATT TATATGGCAG GTTGGAGAAA GATGGCCTCC TGAAAAAACT TGAAGAGGAA
    AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA AGGCTTGACT GTTGTAATGA CGGGTGCTCA
    GATTCCATGG TAGGGCACAA TGAAGAGGCA TCTGGCCACA ACGGTGAGAC CAAGACCCCC
    AGGCCCAGCA GCACCCGGGG CAGTAGTGGA AGCAGAGGTG GTGGAGGCAG CAGCAGCAGC
    AGCAGCGAGC TTTCTACCCC AGAGAAACCC CCACACCAGA GGGCAGGCCC ATTCAGCAGT
    CGGTGGGAGA CGACTATGGG AGAAGCGTCT GCCAGCATCC CCACCACTGT GGGCTCACTT
    CCCAGTTCAA AAAGCTTCCT GGGTATGAAG GCTCGAGAGT TATTTCGTAA TAAGAGCGAG
    AGCCAGTGTG ATGAGGACGG CATGACCAGT AGCCTTTCTG AGAGCCTAAA GACAGAACTG
    GGCAAAGACT TGGGTGTGGA AGCCAAGATT CCCCTGAACC TAGATGGCCC TCACCCGTCT
    CCCCCGACCC CGGACAGTGT TGGACAGCTA CATATCATGG ACTACAATGA GACTCATCAT
    GAACACAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc13015
    Clone ID Related Accession (Same CDS sequence) XM_016961955.2 , XM_016961955.1
    Accession Version XM_016961955.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3492bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product hamartin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006476.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGTCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAACT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
    GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
    AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
    CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
    CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
    CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
    TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
    AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
    ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
    CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GACGTTGTTA
    AATACGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
    CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
    TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
    GGTGGAAAAG GAACTCCTCT GGGAACCCCA GCAACCTCTC CTCCTCCAGC CCCACTCTGT
    CATTCGGATG ACTACGTGCA CATTTCACTC CCCCAGGCCA CAGTCACACC CCCCAGGAAG
    GAAGAGAGAA TGGATTCTGC AAGACCATGT CTACACAGAC AACACCATCT TCTGAATGAC
    AGAGGATCAG AAGAGCCACC TGGCAGCAAA GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG
    TTTTTAGGTG ATCTGGCCTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCTGCA
    ATATCTAGAG AACTTTCTGA GATCACCACA GCAGAGGCAG AGCCTGTGGT TCCTCGAGGA
    GGCTTTGACT CTCCCTTTTA CCGAGACAGT CTCCCAGGTT CTCAGCGGAA GACCCACTCG
    GCAGCCTCCA GTTCTCAGGG CGCCAGCGTG AACCCTGAGC CTTTACACTC CTCCCTGGAC
    AAGCTTGGGC CTGACACACC AAAGCAAGCC TTTACTCCCA TAGACCTGCC CTGCGGCAGT
    GCTGATGAAA GCCCTGCGGG AGACAGGGAA TGCCAGACTT CTTTGGAGAC CAGTATCTTC
    ACTCCCAGTC CTTGTAAAAT TCCACCTCCG ACGAGAGTGG GCTTTGGAAG CGGGCAGCCT
    CCCCCGTATG ATCATCTTTT TGAGGTGGCA TTGCCAAAGA CAGCCCATCA TTTTGTCATC
    AGGAAGACTG AGGAGCTGTT AAAGAAAGCA AAAGGAAACA CAGAGGAAGA TGGTGTGCCC
    TCTACCTCCC CAATGGAAGT GCTGGACAGA CTGATACAGC AGGGAGCAGA CGCACACAGC
    AAGGAGCTGA ACAAGTTGCC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTTGGAGGC
    TCTCCTCCTT CAGATGAGAT CCGCACCCTC CGAGACCAGT TGCTTTTACT GCACAACCAG
    TTACTCTATG AGCGTTTTAA GAGGCAGCAG CATGCCCTCC GGAACAGGCG GCTCCTCCGC
    AAGGTGATCA AAGCAGCAGC TCTGGAGGAA CATAATGCTG CCATGAAAGA TCAGTTGAAG
    TTACAAGAGA AGGACATCCA GATGTGGAAA GTTAGTCTGC AGAAAGAACA AGCTAGATAC
    AATCAGCTCC AGGAGCAGCG TGACACTATG GTAACCAAGC TCCACAGCCA GATCAGACAG
    CTGCAGCACG ACCGAGAGGA ATTCTACAAC CAGAGCCAGG AATTACAGAC GAAGCTGGAG
    GACTGCAGGA ACATGATTGC AGAGCTGCGG ATAGAATTGA AGAAGGCCAA CAACAAGGTG
    TGTCACACTG AGCTGCTGCT CAGTCAGGTT TCCCAAAAGC TCTCAAACAG TGAGTCGGTC
    CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAGGT CAACGAGCTC
    TATTTGGAAC AACTGCAGAA CAAGCACTCA GATACCACAA AGGAAGTAGA AATGATGAAA
    GCCGCCTATC GGAAAGAGCT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GACTCAGAGG
    CTTGATACCT CCCAAAAACG GATTTTGGAA CTGGAATCTC ACCTGGCCAA GAAAGACCAC
    CTTCTTTTGG AACAGAAGAA ATATCTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG
    CAGGCCGCAG AGAGCAGGTA TGAGGCTCAG AAAAGGATAA CCCAGGTGTT TGAATTGGAG
    ATCTTAGATT TATATGGCAG GTTGGAGAAA GATGGCCTCC TGAAAAAACT TGAAGAGGAA
    AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA AGGCTTGACT GTTGTAATGA CGGGTGCTCA
    GATTCCATGG TAGGGCACAA TGAAGAGGCA TCTGGCCACA ACGGTGAGAC CAAGACCCCC
    AGGCCCAGCA GCACCCGGGG CAGTAGTGGA AGCAGAGGTG GTGGAGGCAG CAGCAGCAGC
    AGCAGCGAGC TTTCTACCCC AGAGAAACCC CCACACCAGA GGGCAGGCCC ATTCAGCAGT
    CGGTGGGAGA CGACTATGGG AGAAGCGTCT GCCAGCATCC CCACCACTGT GGGCTCACTT
    CCCAGTTCAA AAAGCTTCCT GGGTATGAAG GCTCGAGAGT TATTTCGTAA TAAGAGCGAG
    AGCCAGTGTG ATGAGGACGG CATGACCAGT AGCCTTTCTG AGAGCCTAAA GACAGAACTG
    GGCAAAGACT TGGGTGTGGA AGCCAAGATT CCCCTGAACC TAGATGGCCC TCACCCGTCT
    CCCCCGACCC CGGACAGTGT TGGACAGCTA CATATCATGG ACTACAATGA GACTCATCAT
    GAACACAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016817444.1
    CDS411..3902
    Translation

    Target ORF information:

    RefSeq Version XM_016961955.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes tuberous sclerosis 1 (TSC1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016961955.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGTCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAACT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
    GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
    AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
    CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
    CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
    CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
    TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
    AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
    ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
    CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GACGTTGTTA
    AATACGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
    CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
    TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
    GGTGGAAAAG GAACTCCTCT GGGAACCCCA GCAACCTCTC CTCCTCCAGC CCCACTCTGT
    CATTCGGATG ACTACGTGCA CATTTCACTC CCCCAGGCCA CAGTCACACC CCCCAGGAAG
    GAAGAGAGAA TGGATTCTGC AAGACCATGT CTACACAGAC AACACCATCT TCTGAATGAC
    AGAGGATCAG AAGAGCCACC TGGCAGCAAA GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG
    TTTTTAGGTG ATCTGGCCTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCTGCA
    ATATCTAGAG AACTTTCTGA GATCACCACA GCAGAGGCAG AGCCTGTGGT TCCTCGAGGA
    GGCTTTGACT CTCCCTTTTA CCGAGACAGT CTCCCAGGTT CTCAGCGGAA GACCCACTCG
    GCAGCCTCCA GTTCTCAGGG CGCCAGCGTG AACCCTGAGC CTTTACACTC CTCCCTGGAC
    AAGCTTGGGC CTGACACACC AAAGCAAGCC TTTACTCCCA TAGACCTGCC CTGCGGCAGT
    GCTGATGAAA GCCCTGCGGG AGACAGGGAA TGCCAGACTT CTTTGGAGAC CAGTATCTTC
    ACTCCCAGTC CTTGTAAAAT TCCACCTCCG ACGAGAGTGG GCTTTGGAAG CGGGCAGCCT
    CCCCCGTATG ATCATCTTTT TGAGGTGGCA TTGCCAAAGA CAGCCCATCA TTTTGTCATC
    AGGAAGACTG AGGAGCTGTT AAAGAAAGCA AAAGGAAACA CAGAGGAAGA TGGTGTGCCC
    TCTACCTCCC CAATGGAAGT GCTGGACAGA CTGATACAGC AGGGAGCAGA CGCACACAGC
    AAGGAGCTGA ACAAGTTGCC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTTGGAGGC
    TCTCCTCCTT CAGATGAGAT CCGCACCCTC CGAGACCAGT TGCTTTTACT GCACAACCAG
    TTACTCTATG AGCGTTTTAA GAGGCAGCAG CATGCCCTCC GGAACAGGCG GCTCCTCCGC
    AAGGTGATCA AAGCAGCAGC TCTGGAGGAA CATAATGCTG CCATGAAAGA TCAGTTGAAG
    TTACAAGAGA AGGACATCCA GATGTGGAAA GTTAGTCTGC AGAAAGAACA AGCTAGATAC
    AATCAGCTCC AGGAGCAGCG TGACACTATG GTAACCAAGC TCCACAGCCA GATCAGACAG
    CTGCAGCACG ACCGAGAGGA ATTCTACAAC CAGAGCCAGG AATTACAGAC GAAGCTGGAG
    GACTGCAGGA ACATGATTGC AGAGCTGCGG ATAGAATTGA AGAAGGCCAA CAACAAGGTG
    TGTCACACTG AGCTGCTGCT CAGTCAGGTT TCCCAAAAGC TCTCAAACAG TGAGTCGGTC
    CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAGGT CAACGAGCTC
    TATTTGGAAC AACTGCAGAA CAAGCACTCA GATACCACAA AGGAAGTAGA AATGATGAAA
    GCCGCCTATC GGAAAGAGCT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GACTCAGAGG
    CTTGATACCT CCCAAAAACG GATTTTGGAA CTGGAATCTC ACCTGGCCAA GAAAGACCAC
    CTTCTTTTGG AACAGAAGAA ATATCTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG
    CAGGCCGCAG AGAGCAGGTA TGAGGCTCAG AAAAGGATAA CCCAGGTGTT TGAATTGGAG
    ATCTTAGATT TATATGGCAG GTTGGAGAAA GATGGCCTCC TGAAAAAACT TGAAGAGGAA
    AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA AGGCTTGACT GTTGTAATGA CGGGTGCTCA
    GATTCCATGG TAGGGCACAA TGAAGAGGCA TCTGGCCACA ACGGTGAGAC CAAGACCCCC
    AGGCCCAGCA GCACCCGGGG CAGTAGTGGA AGCAGAGGTG GTGGAGGCAG CAGCAGCAGC
    AGCAGCGAGC TTTCTACCCC AGAGAAACCC CCACACCAGA GGGCAGGCCC ATTCAGCAGT
    CGGTGGGAGA CGACTATGGG AGAAGCGTCT GCCAGCATCC CCACCACTGT GGGCTCACTT
    CCCAGTTCAA AAAGCTTCCT GGGTATGAAG GCTCGAGAGT TATTTCGTAA TAAGAGCGAG
    AGCCAGTGTG ATGAGGACGG CATGACCAGT AGCCTTTCTG AGAGCCTAAA GACAGAACTG
    GGCAAAGACT TGGGTGTGGA AGCCAAGATT CCCCTGAACC TAGATGGCCC TCACCCGTCT
    CCCCCGACCC CGGACAGTGT TGGACAGCTA CATATCATGG ACTACAATGA GACTCATCAT
    GAACACAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.