NCKAP1 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene
Clones
gRNAs

Summary

Gene Symbol	NCKAP1
Entrez Gene ID	459801
Full Name	NCK associated protein 1
General protein information	Preferred Names NCK associated protein 1 Names nck-associated protein 1
Gene Type	protein-coding
Organism	Pan troglodytes(chimpanzee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001148485.5	XP_001148485.2	nck-associated protein 1 isoform X1
XM_016950143.1	XP_016805632.1	nck-associated protein 1 isoform X2
XM_001148485.6	XP_001148485.2	nck-associated protein 1 isoform X1
XM_003309356.3	XP_003309404.1	nck-associated protein 1 isoform X3
XM_016950144.1	XP_016805633.1	nck-associated protein 1 isoform X4
XM_016950143.2	XP_016805632.1	nck-associated protein 1 isoform X2
XM_003309356.4	XP_003309404.1	nck-associated protein 1 isoform X3
XM_016950144.2	XP_016805633.1	nck-associated protein 1 isoform X4

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NCKAP1	XP_535990.3
Xenopus tropicalis (tropical clawed frog)	nckap1	XP_004917966.1
Pan troglodytes (chimpanzee)	NCKAP1	XP_001148485.2
Rattus norvegicus (Norway rat)	Nckap1	NP_113806.1
Drosophila melanogaster (fruit fly)	Hem	NP_524214.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004946	XP_315044.2
	NCKAP1	XP_001099879.2
Bos taurus (cattle)	LOC101907541	XP_005202363.1
Mus musculus (house mouse)	Nckap1	NP_058661.1
Arabidopsis thaliana (thale cress)	GRL	NP_001031485.1
Oryza sativa (rice)	Os08g0544500	NP_001062406.2
Homo sapiens (human)	NCKAP1	NP_995314.1
Gallus gallus (chicken)	NCKAP1	XP_001232275.2
Danio rerio (zebrafish)	nckap1	NP_001131137.1
Caenorhabditis elegans (roundworm)	gex-3	NP_502368.1

Gene Ontology

The following NCKAP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCKAP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPc80410	XM_016950143.2 Latest version!	Pan troglodytes NCK associated protein 1 (NCKAP1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPc80411	XM_016950144.2 Latest version!	Pan troglodytes NCK associated protein 1 (NCKAP1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPc05680	XM_003309356.4 Latest version!	Pan troglodytes NCK associated protein 1 (NCKAP1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OPc05681	XM_001148485.6 Latest version!	Pan troglodytes NCK associated protein 1 (NCKAP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OPc05681	XM_001148485.5	Pan troglodytes NCK associated protein 1 (NCKAP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OPc80410	XM_016950143.1	Pan troglodytes NCK associated protein 1 (NCKAP1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPc05680	XM_003309356.3	Pan troglodytes NCK associated protein 1 (NCKAP1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OPc80411	XM_016950144.1	Pan troglodytes NCK associated protein 1 (NCKAP1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCKAP1 ( XM_016950143.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016805632.1 Pan troglodytes NCK associated protein 1 (NCKAP1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPc80410
Clone ID Related Accession (Same CDS sequence)	XM_016950143.1 , XM_016950143.2
Accession Version	XM_016950143.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	nck-associated protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006470.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCGCT CAGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGCA AGGACAAGTT 
TGGAAGGCAT GTGGAGACCC CAAGGCAAAA CCATCCTATC TTATCGACAA AAACCTGGAA 
TCTGCTGTGA AATTCATAGT CAGAAAATTC CCTGCTGTAG AAACCCGCAA CAACAATCTT 
GCACAACTAC AGAAAGAAAA ATCAGAGATT CTGAAAAATC TGGCATTATA TTACTTCACA 
TTTGTAGATG TTATGGAATT TAAGGACCAT GTTTGTGAAT TGCTGAATAC TATTGACGTT 
TGCCAAGTCT TCTTTGATAT TACTGTAAAC TTTGATTTAA CAAAGAACTA CTTAGATTTA 
ATTATAACCT ATACAACACT AATGATACTG CTGTCTCGAA TTGAAGAAAG GAAGGCAATC 
ATTGGATTAT ACAACTATGC CCATGAAATG ACTCATGGAG CAAGTGACAG AGAATACCCA 
CGCCTTGGCC AGATGATTGT GGATTATGAA AACCCTTTAA AGAAGATGAT GGAAGAATTT 
GTACCCCATA GCAAGTCTCT TTCAGATGCA CTAATTTCTC TTCAAATGGT ATATCCTCGA 
AGGAATCTTT CAGCTGACCA GTGGAGAAAT GCCCAGTTAT TGAGCCTCAT CAGTGCGCCT 
AGTACAATGC TTAATCCAGC GCAGTCCGAC ACTATGCCTT GTGAATACCT CTCTTTGGAT 
GCAATGGAAA AGTGGATTAT CTTTGGCTTT ATTTTGTGCC ATGGGATCCT AAATACTGAC 
GCTACAGCAC TGAACCTTTG GAAACTAGCT CTTCAAAGTA GCTCTTGCCT CTCTCTCTTT 
CGGGATGAAG TTTTCCACAT TCACAAAGCT GCAGAAGACT TATTTGTAAA CATACGAGGC 
TATAATAAAC GTATTAATGA CATAAGAGAA TGCAAGGAGG CAGCCGTGTC ACATGCTGGT 
TCAATGCACA GAGAAAGACG CAAGTTTTTA AGATCTGCAC TGAAGGAATT GGCTACTGTC 
CTCTCTGATC AACCTGGATT GCTAGGTCCC AAGGCACTTT TTGTTTTTAT GGCATTATCC 
TTTGCCCGTG ATGAAATCAT CTGGCTACTT CGTCATGCAG ATAACATGCC AAAGAAGAGT 
GCAGACGACT TTATAGATAA GCACATTGCT GAATTAATAT TTTACATGGA AGAACTTAGA 
GCACATGTGA GGAAATATGG ACCTGTAATG CAGAGGTATT ACGTGCAGTA CCTTTCTGGC 
TTTGATGCTG TTGTCCTCAA TGAACTCGTG CAGAATCTTT CTGTTTGCCC TGAAGATGAA 
TCAATCATCA TGTCCTCTTT TGTTAACACT ATGACTTCCC TAAGTGTAAA ACAAGTTGAA 
GATGGGGAAG TATTTGATTT CAGAGGAATG AGATTAGATT GGTTTAGGTT ACAGGCATAT 
ACTAGTGTCT CAAAGGCTTC ACTTGGCCTT GCAGATCACA GAGAACTTGG AAAGATGATG 
AATACAATAA TTTTTCATAC AAAAATGGTA GATTCCTTGG TGGAAATGTT GGTGGAAACA 
TCAGATCTCT CCATATTTTG TTTTTATAGT CGTGCTTTTG AGAAGATGTT TCAACAGTGT 
TTGGAGTTAC CCTCTCAATC AAGATACTCA ATTGCATTTC CACTACTTTG CACTCATTTT 
ATGAGTTGCA CACATGAACT GTGTCCAGAA GAGCGACATC ATATTGGAGA TCGCAGTCTT 
TCCTTATGTA ATATGTTCCT AGATGAAATG GCCAAACAAG CTCGAAATCT CATCACTGAT 
ATTTGCACAG AACAGTGTAC CCTTAGTGAC CAGTTGCTGC CCAAGCATTG TGCCAAAACT 
ATCAGTCAAG CAGTGAATAA GAAATCAAAA AAGCAGACTG GTAAGAAAGG GGAACCTGAA 
AGGGAGAAAC CAGGTGTTGA GAGCATGAGG AAAAACAGGC TGGTTGTGAC CAACCTTGAT 
AAATTGCACA CTGCACTTTC TGAGTTATGC TTCTCTATAA ATTATGTACC AAACATGGTG 
GTATGGGAAC ATACCTTTAC CCCACGAGAA TATTTGACTT CTCATCTGGA AATACGCTTT 
ACCAAGTCAA TTGTTGGGAT GACTATGTAT AATCAAGCCA CACAGGAAAT TGCAAAACCT 
TCAGAACTTC TAACAAGTGT AAGAGCATAC ATGACCGTAC TCCAGTCAAT AGAAAACTAT 
GTGCAGATTG ATATTACAAG AGTATTTAAT AATGTGCTTC TTCAACAAAC ACAACATTTA 
GACAGTCATG GAGAGCCAAC CATTACAAGT CTATACACAA ATTGGTATTT GGAAACTTTG 
TTACGACAAG TCAGCAATGG CCATATAGCA TATTTTCCTG CAATGAAAGC ATTTGTGAAC 
TTACCTACAG AAAATGAATT AACATTCAAT GCAGAGGAAT ATTCTGACAT ATCAGAAATG 
AGGTCATTAT CAGAACTACT AGGCCCATAT GGTATGAAGT TTCTAAGTGA AAGCCTTATG 
TGGCATATTT CGTCACAAGT TGCTGAACTT AAGAAACTTG TGGTGGAGAA TGTTGATGTG 
TTAACACAAA TGAGGACCAG CTTTGACAAA CCAGACCAGA TGGCTGCACT CTTTAAAAGA 
TTATCATCTG TTGACAGTGT CTTGAAGAGG ATGACAATAA TTGGTGTAAT TTTATCCTTC 
CGATCATTGG CACAAGAAGC ACTTAGAGAT GTCTTATCCT ACCACATTCC TTTTCTTGTA 
AGTTCAATTG AAGATTTTAA GGATCACATT CCAAGGGAAA CTGATATGAA GGTTGCAATG 
AATGTGTATG AGTTATCATC AGCTGCCGGA TTACCTTGTG AGATTGATCC TGCATTGGTC 
GTAGCTCTTT CTTCACAAAA ATCGGAAAAC ATTAGTCCAG AAGAAGAGTA TAAAATTGCC 
TGCCTTCTCA TGGTGTTTGT GGCAGTTTCT TTGCCAACAC TGGCCAGTAA TGTGATGTCT 
CAGTACAGCC CTGCTATAGA AGGGCATTGC AACAACATAC ATTGCTTGGC CAAAGCCATC 
AACCAGATTG CTGCAGCTTT GTTTACAATT CACAAAGGAA GCATTGAAGA CCGTCTTAAA 
GAATTTCTGG CGCTTGCATC CTCCAGTCTA CTGAAAATTG GCCAGGAGAC AGATAAAACT 
ACAACAAGAA ATAGAGAATC CGTTTATTTA CTGCTAGATA TGATTGTACA AGAATCTCCA 
TTCCTTACAA TGGATCTTTT GGAATCTTGT TTTCCTTATG TCTTGCTGAG AAATGCATAC 
CATGCTGTCT ACAAACAAAG TGTTACATCT TCTGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016805632.1
CDS	367..3765
Translation	MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKQGQVWKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNYLDLIITYTTLMILLSRIEERKAIIGLYNYAHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNTDATALNLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSHAGSMHRERRKFLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLGLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYVPNMVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA

Target ORF information:

RefSeq Version	XM_016950143.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes NCK associated protein 1 (NCKAP1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016950143.1

ORF Insert Sequence:

ATGTCGCGCT CAGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGCA AGGACAAGTT 
TGGAAGGCAT GTGGAGACCC CAAGGCAAAA CCATCCTATC TTATCGACAA AAACCTGGAA 
TCTGCTGTGA AATTCATAGT CAGAAAATTC CCTGCTGTAG AAACCCGCAA CAACAATCTT 
GCACAACTAC AGAAAGAAAA ATCAGAGATT CTGAAAAATC TGGCATTATA TTACTTCACA 
TTTGTAGATG TTATGGAATT TAAGGACCAT GTTTGTGAAT TGCTGAATAC TATTGACGTT 
TGCCAAGTCT TCTTTGATAT TACTGTAAAC TTTGATTTAA CAAAGAACTA CTTAGATTTA 
ATTATAACCT ATACAACACT AATGATACTG CTGTCTCGAA TTGAAGAAAG GAAGGCAATC 
ATTGGATTAT ACAACTATGC CCATGAAATG ACTCATGGAG CAAGTGACAG AGAATACCCA 
CGCCTTGGCC AGATGATTGT GGATTATGAA AACCCTTTAA AGAAGATGAT GGAAGAATTT 
GTACCCCATA GCAAGTCTCT TTCAGATGCA CTAATTTCTC TTCAAATGGT ATATCCTCGA 
AGGAATCTTT CAGCTGACCA GTGGAGAAAT GCCCAGTTAT TGAGCCTCAT CAGTGCGCCT 
AGTACAATGC TTAATCCAGC GCAGTCCGAC ACTATGCCTT GTGAATACCT CTCTTTGGAT 
GCAATGGAAA AGTGGATTAT CTTTGGCTTT ATTTTGTGCC ATGGGATCCT AAATACTGAC 
GCTACAGCAC TGAACCTTTG GAAACTAGCT CTTCAAAGTA GCTCTTGCCT CTCTCTCTTT 
CGGGATGAAG TTTTCCACAT TCACAAAGCT GCAGAAGACT TATTTGTAAA CATACGAGGC 
TATAATAAAC GTATTAATGA CATAAGAGAA TGCAAGGAGG CAGCCGTGTC ACATGCTGGT 
TCAATGCACA GAGAAAGACG CAAGTTTTTA AGATCTGCAC TGAAGGAATT GGCTACTGTC 
CTCTCTGATC AACCTGGATT GCTAGGTCCC AAGGCACTTT TTGTTTTTAT GGCATTATCC 
TTTGCCCGTG ATGAAATCAT CTGGCTACTT CGTCATGCAG ATAACATGCC AAAGAAGAGT 
GCAGACGACT TTATAGATAA GCACATTGCT GAATTAATAT TTTACATGGA AGAACTTAGA 
GCACATGTGA GGAAATATGG ACCTGTAATG CAGAGGTATT ACGTGCAGTA CCTTTCTGGC 
TTTGATGCTG TTGTCCTCAA TGAACTCGTG CAGAATCTTT CTGTTTGCCC TGAAGATGAA 
TCAATCATCA TGTCCTCTTT TGTTAACACT ATGACTTCCC TAAGTGTAAA ACAAGTTGAA 
GATGGGGAAG TATTTGATTT CAGAGGAATG AGATTAGATT GGTTTAGGTT ACAGGCATAT 
ACTAGTGTCT CAAAGGCTTC ACTTGGCCTT GCAGATCACA GAGAACTTGG AAAGATGATG 
AATACAATAA TTTTTCATAC AAAAATGGTA GATTCCTTGG TGGAAATGTT GGTGGAAACA 
TCAGATCTCT CCATATTTTG TTTTTATAGT CGTGCTTTTG AGAAGATGTT TCAACAGTGT 
TTGGAGTTAC CCTCTCAATC AAGATACTCA ATTGCATTTC CACTACTTTG CACTCATTTT 
ATGAGTTGCA CACATGAACT GTGTCCAGAA GAGCGACATC ATATTGGAGA TCGCAGTCTT 
TCCTTATGTA ATATGTTCCT AGATGAAATG GCCAAACAAG CTCGAAATCT CATCACTGAT 
ATTTGCACAG AACAGTGTAC CCTTAGTGAC CAGTTGCTGC CCAAGCATTG TGCCAAAACT 
ATCAGTCAAG CAGTGAATAA GAAATCAAAA AAGCAGACTG GTAAGAAAGG GGAACCTGAA 
AGGGAGAAAC CAGGTGTTGA GAGCATGAGG AAAAACAGGC TGGTTGTGAC CAACCTTGAT 
AAATTGCACA CTGCACTTTC TGAGTTATGC TTCTCTATAA ATTATGTACC AAACATGGTG 
GTATGGGAAC ATACCTTTAC CCCACGAGAA TATTTGACTT CTCATCTGGA AATACGCTTT 
ACCAAGTCAA TTGTTGGGAT GACTATGTAT AATCAAGCCA CACAGGAAAT TGCAAAACCT 
TCAGAACTTC TAACAAGTGT AAGAGCATAC ATGACCGTAC TCCAGTCAAT AGAAAACTAT 
GTGCAGATTG ATATTACAAG AGTATTTAAT AATGTGCTTC TTCAACAAAC ACAACATTTA 
GACAGTCATG GAGAGCCAAC CATTACAAGT CTATACACAA ATTGGTATTT GGAAACTTTG 
TTACGACAAG TCAGCAATGG CCATATAGCA TATTTTCCTG CAATGAAAGC ATTTGTGAAC 
TTACCTACAG AAAATGAATT AACATTCAAT GCAGAGGAAT ATTCTGACAT ATCAGAAATG 
AGGTCATTAT CAGAACTACT AGGCCCATAT GGTATGAAGT TTCTAAGTGA AAGCCTTATG 
TGGCATATTT CGTCACAAGT TGCTGAACTT AAGAAACTTG TGGTGGAGAA TGTTGATGTG 
TTAACACAAA TGAGGACCAG CTTTGACAAA CCAGACCAGA TGGCTGCACT CTTTAAAAGA 
TTATCATCTG TTGACAGTGT CTTGAAGAGG ATGACAATAA TTGGTGTAAT TTTATCCTTC 
CGATCATTGG CACAAGAAGC ACTTAGAGAT GTCTTATCCT ACCACATTCC TTTTCTTGTA 
AGTTCAATTG AAGATTTTAA GGATCACATT CCAAGGGAAA CTGATATGAA GGTTGCAATG 
AATGTGTATG AGTTATCATC AGCTGCCGGA TTACCTTGTG AGATTGATCC TGCATTGGTC 
GTAGCTCTTT CTTCACAAAA ATCGGAAAAC ATTAGTCCAG AAGAAGAGTA TAAAATTGCC 
TGCCTTCTCA TGGTGTTTGT GGCAGTTTCT TTGCCAACAC TGGCCAGTAA TGTGATGTCT 
CAGTACAGCC CTGCTATAGA AGGGCATTGC AACAACATAC ATTGCTTGGC CAAAGCCATC 
AACCAGATTG CTGCAGCTTT GTTTACAATT CACAAAGGAA GCATTGAAGA CCGTCTTAAA 
GAATTTCTGG CGCTTGCATC CTCCAGTCTA CTGAAAATTG GCCAGGAGAC AGATAAAACT 
ACAACAAGAA ATAGAGAATC CGTTTATTTA CTGCTAGATA TGATTGTACA AGAATCTCCA 
TTCCTTACAA TGGATCTTTT GGAATCTTGT TTTCCTTATG TCTTGCTGAG AAATGCATAC 
CATGCTGTCT ACAAACAAAG TGTTACATCT TCTGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP1 ( XM_016950143.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016805632.1 Pan troglodytes NCK associated protein 1 (NCKAP1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPc80410
Clone ID Related Accession (Same CDS sequence)	XM_016950143.1 , XM_016950143.2
Accession Version	XM_016950143.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	nck-associated protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036881.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016950143.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCGCT CAGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGCA AGGACAAGTT 
TGGAAGGCAT GTGGAGACCC CAAGGCAAAA CCATCCTATC TTATCGACAA AAACCTGGAA 
TCTGCTGTGA AATTCATAGT CAGAAAATTC CCTGCTGTAG AAACCCGCAA CAACAATCTT 
GCACAACTAC AGAAAGAAAA ATCAGAGATT CTGAAAAATC TGGCATTATA TTACTTCACA 
TTTGTAGATG TTATGGAATT TAAGGACCAT GTTTGTGAAT TGCTGAATAC TATTGACGTT 
TGCCAAGTCT TCTTTGATAT TACTGTAAAC TTTGATTTAA CAAAGAACTA CTTAGATTTA 
ATTATAACCT ATACAACACT AATGATACTG CTGTCTCGAA TTGAAGAAAG GAAGGCAATC 
ATTGGATTAT ACAACTATGC CCATGAAATG ACTCATGGAG CAAGTGACAG AGAATACCCA 
CGCCTTGGCC AGATGATTGT GGATTATGAA AACCCTTTAA AGAAGATGAT GGAAGAATTT 
GTACCCCATA GCAAGTCTCT TTCAGATGCA CTAATTTCTC TTCAAATGGT ATATCCTCGA 
AGGAATCTTT CAGCTGACCA GTGGAGAAAT GCCCAGTTAT TGAGCCTCAT CAGTGCGCCT 
AGTACAATGC TTAATCCAGC GCAGTCCGAC ACTATGCCTT GTGAATACCT CTCTTTGGAT 
GCAATGGAAA AGTGGATTAT CTTTGGCTTT ATTTTGTGCC ATGGGATCCT AAATACTGAC 
GCTACAGCAC TGAACCTTTG GAAACTAGCT CTTCAAAGTA GCTCTTGCCT CTCTCTCTTT 
CGGGATGAAG TTTTCCACAT TCACAAAGCT GCAGAAGACT TATTTGTAAA CATACGAGGC 
TATAATAAAC GTATTAATGA CATAAGAGAA TGCAAGGAGG CAGCCGTGTC ACATGCTGGT 
TCAATGCACA GAGAAAGACG CAAGTTTTTA AGATCTGCAC TGAAGGAATT GGCTACTGTC 
CTCTCTGATC AACCTGGATT GCTAGGTCCC AAGGCACTTT TTGTTTTTAT GGCATTATCC 
TTTGCCCGTG ATGAAATCAT CTGGCTACTT CGTCATGCAG ATAACATGCC AAAGAAGAGT 
GCAGACGACT TTATAGATAA GCACATTGCT GAATTAATAT TTTACATGGA AGAACTTAGA 
GCACATGTGA GGAAATATGG ACCTGTAATG CAGAGGTATT ACGTGCAGTA CCTTTCTGGC 
TTTGATGCTG TTGTCCTCAA TGAACTCGTG CAGAATCTTT CTGTTTGCCC TGAAGATGAA 
TCAATCATCA TGTCCTCTTT TGTTAACACT ATGACTTCCC TAAGTGTAAA ACAAGTTGAA 
GATGGGGAAG TATTTGATTT CAGAGGAATG AGATTAGATT GGTTTAGGTT ACAGGCATAT 
ACTAGTGTCT CAAAGGCTTC ACTTGGCCTT GCAGATCACA GAGAACTTGG AAAGATGATG 
AATACAATAA TTTTTCATAC AAAAATGGTA GATTCCTTGG TGGAAATGTT GGTGGAAACA 
TCAGATCTCT CCATATTTTG TTTTTATAGT CGTGCTTTTG AGAAGATGTT TCAACAGTGT 
TTGGAGTTAC CCTCTCAATC AAGATACTCA ATTGCATTTC CACTACTTTG CACTCATTTT 
ATGAGTTGCA CACATGAACT GTGTCCAGAA GAGCGACATC ATATTGGAGA TCGCAGTCTT 
TCCTTATGTA ATATGTTCCT AGATGAAATG GCCAAACAAG CTCGAAATCT CATCACTGAT 
ATTTGCACAG AACAGTGTAC CCTTAGTGAC CAGTTGCTGC CCAAGCATTG TGCCAAAACT 
ATCAGTCAAG CAGTGAATAA GAAATCAAAA AAGCAGACTG GTAAGAAAGG GGAACCTGAA 
AGGGAGAAAC CAGGTGTTGA GAGCATGAGG AAAAACAGGC TGGTTGTGAC CAACCTTGAT 
AAATTGCACA CTGCACTTTC TGAGTTATGC TTCTCTATAA ATTATGTACC AAACATGGTG 
GTATGGGAAC ATACCTTTAC CCCACGAGAA TATTTGACTT CTCATCTGGA AATACGCTTT 
ACCAAGTCAA TTGTTGGGAT GACTATGTAT AATCAAGCCA CACAGGAAAT TGCAAAACCT 
TCAGAACTTC TAACAAGTGT AAGAGCATAC ATGACCGTAC TCCAGTCAAT AGAAAACTAT 
GTGCAGATTG ATATTACAAG AGTATTTAAT AATGTGCTTC TTCAACAAAC ACAACATTTA 
GACAGTCATG GAGAGCCAAC CATTACAAGT CTATACACAA ATTGGTATTT GGAAACTTTG 
TTACGACAAG TCAGCAATGG CCATATAGCA TATTTTCCTG CAATGAAAGC ATTTGTGAAC 
TTACCTACAG AAAATGAATT AACATTCAAT GCAGAGGAAT ATTCTGACAT ATCAGAAATG 
AGGTCATTAT CAGAACTACT AGGCCCATAT GGTATGAAGT TTCTAAGTGA AAGCCTTATG 
TGGCATATTT CGTCACAAGT TGCTGAACTT AAGAAACTTG TGGTGGAGAA TGTTGATGTG 
TTAACACAAA TGAGGACCAG CTTTGACAAA CCAGACCAGA TGGCTGCACT CTTTAAAAGA 
TTATCATCTG TTGACAGTGT CTTGAAGAGG ATGACAATAA TTGGTGTAAT TTTATCCTTC 
CGATCATTGG CACAAGAAGC ACTTAGAGAT GTCTTATCCT ACCACATTCC TTTTCTTGTA 
AGTTCAATTG AAGATTTTAA GGATCACATT CCAAGGGAAA CTGATATGAA GGTTGCAATG 
AATGTGTATG AGTTATCATC AGCTGCCGGA TTACCTTGTG AGATTGATCC TGCATTGGTC 
GTAGCTCTTT CTTCACAAAA ATCGGAAAAC ATTAGTCCAG AAGAAGAGTA TAAAATTGCC 
TGCCTTCTCA TGGTGTTTGT GGCAGTTTCT TTGCCAACAC TGGCCAGTAA TGTGATGTCT 
CAGTACAGCC CTGCTATAGA AGGGCATTGC AACAACATAC ATTGCTTGGC CAAAGCCATC 
AACCAGATTG CTGCAGCTTT GTTTACAATT CACAAAGGAA GCATTGAAGA CCGTCTTAAA 
GAATTTCTGG CGCTTGCATC CTCCAGTCTA CTGAAAATTG GCCAGGAGAC AGATAAAACT 
ACAACAAGAA ATAGAGAATC CGTTTATTTA CTGCTAGATA TGATTGTACA AGAATCTCCA 
TTCCTTACAA TGGATCTTTT GGAATCTTGT TTTCCTTATG TCTTGCTGAG AAATGCATAC 
CATGCTGTCT ACAAACAAAG TGTTACATCT TCTGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016805632.1
CDS	84..3482
Translation	MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKQGQVWKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNYLDLIITYTTLMILLSRIEERKAIIGLYNYAHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNTDATALNLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSHAGSMHRERRKFLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLGLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYVPNMVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA

Target ORF information:

RefSeq Version	XM_016950143.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes NCK associated protein 1 (NCKAP1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016950143.2

ORF Insert Sequence:

ATGTCGCGCT CAGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGCA AGGACAAGTT 
TGGAAGGCAT GTGGAGACCC CAAGGCAAAA CCATCCTATC TTATCGACAA AAACCTGGAA 
TCTGCTGTGA AATTCATAGT CAGAAAATTC CCTGCTGTAG AAACCCGCAA CAACAATCTT 
GCACAACTAC AGAAAGAAAA ATCAGAGATT CTGAAAAATC TGGCATTATA TTACTTCACA 
TTTGTAGATG TTATGGAATT TAAGGACCAT GTTTGTGAAT TGCTGAATAC TATTGACGTT 
TGCCAAGTCT TCTTTGATAT TACTGTAAAC TTTGATTTAA CAAAGAACTA CTTAGATTTA 
ATTATAACCT ATACAACACT AATGATACTG CTGTCTCGAA TTGAAGAAAG GAAGGCAATC 
ATTGGATTAT ACAACTATGC CCATGAAATG ACTCATGGAG CAAGTGACAG AGAATACCCA 
CGCCTTGGCC AGATGATTGT GGATTATGAA AACCCTTTAA AGAAGATGAT GGAAGAATTT 
GTACCCCATA GCAAGTCTCT TTCAGATGCA CTAATTTCTC TTCAAATGGT ATATCCTCGA 
AGGAATCTTT CAGCTGACCA GTGGAGAAAT GCCCAGTTAT TGAGCCTCAT CAGTGCGCCT 
AGTACAATGC TTAATCCAGC GCAGTCCGAC ACTATGCCTT GTGAATACCT CTCTTTGGAT 
GCAATGGAAA AGTGGATTAT CTTTGGCTTT ATTTTGTGCC ATGGGATCCT AAATACTGAC 
GCTACAGCAC TGAACCTTTG GAAACTAGCT CTTCAAAGTA GCTCTTGCCT CTCTCTCTTT 
CGGGATGAAG TTTTCCACAT TCACAAAGCT GCAGAAGACT TATTTGTAAA CATACGAGGC 
TATAATAAAC GTATTAATGA CATAAGAGAA TGCAAGGAGG CAGCCGTGTC ACATGCTGGT 
TCAATGCACA GAGAAAGACG CAAGTTTTTA AGATCTGCAC TGAAGGAATT GGCTACTGTC 
CTCTCTGATC AACCTGGATT GCTAGGTCCC AAGGCACTTT TTGTTTTTAT GGCATTATCC 
TTTGCCCGTG ATGAAATCAT CTGGCTACTT CGTCATGCAG ATAACATGCC AAAGAAGAGT 
GCAGACGACT TTATAGATAA GCACATTGCT GAATTAATAT TTTACATGGA AGAACTTAGA 
GCACATGTGA GGAAATATGG ACCTGTAATG CAGAGGTATT ACGTGCAGTA CCTTTCTGGC 
TTTGATGCTG TTGTCCTCAA TGAACTCGTG CAGAATCTTT CTGTTTGCCC TGAAGATGAA 
TCAATCATCA TGTCCTCTTT TGTTAACACT ATGACTTCCC TAAGTGTAAA ACAAGTTGAA 
GATGGGGAAG TATTTGATTT CAGAGGAATG AGATTAGATT GGTTTAGGTT ACAGGCATAT 
ACTAGTGTCT CAAAGGCTTC ACTTGGCCTT GCAGATCACA GAGAACTTGG AAAGATGATG 
AATACAATAA TTTTTCATAC AAAAATGGTA GATTCCTTGG TGGAAATGTT GGTGGAAACA 
TCAGATCTCT CCATATTTTG TTTTTATAGT CGTGCTTTTG AGAAGATGTT TCAACAGTGT 
TTGGAGTTAC CCTCTCAATC AAGATACTCA ATTGCATTTC CACTACTTTG CACTCATTTT 
ATGAGTTGCA CACATGAACT GTGTCCAGAA GAGCGACATC ATATTGGAGA TCGCAGTCTT 
TCCTTATGTA ATATGTTCCT AGATGAAATG GCCAAACAAG CTCGAAATCT CATCACTGAT 
ATTTGCACAG AACAGTGTAC CCTTAGTGAC CAGTTGCTGC CCAAGCATTG TGCCAAAACT 
ATCAGTCAAG CAGTGAATAA GAAATCAAAA AAGCAGACTG GTAAGAAAGG GGAACCTGAA 
AGGGAGAAAC CAGGTGTTGA GAGCATGAGG AAAAACAGGC TGGTTGTGAC CAACCTTGAT 
AAATTGCACA CTGCACTTTC TGAGTTATGC TTCTCTATAA ATTATGTACC AAACATGGTG 
GTATGGGAAC ATACCTTTAC CCCACGAGAA TATTTGACTT CTCATCTGGA AATACGCTTT 
ACCAAGTCAA TTGTTGGGAT GACTATGTAT AATCAAGCCA CACAGGAAAT TGCAAAACCT 
TCAGAACTTC TAACAAGTGT AAGAGCATAC ATGACCGTAC TCCAGTCAAT AGAAAACTAT 
GTGCAGATTG ATATTACAAG AGTATTTAAT AATGTGCTTC TTCAACAAAC ACAACATTTA 
GACAGTCATG GAGAGCCAAC CATTACAAGT CTATACACAA ATTGGTATTT GGAAACTTTG 
TTACGACAAG TCAGCAATGG CCATATAGCA TATTTTCCTG CAATGAAAGC ATTTGTGAAC 
TTACCTACAG AAAATGAATT AACATTCAAT GCAGAGGAAT ATTCTGACAT ATCAGAAATG 
AGGTCATTAT CAGAACTACT AGGCCCATAT GGTATGAAGT TTCTAAGTGA AAGCCTTATG 
TGGCATATTT CGTCACAAGT TGCTGAACTT AAGAAACTTG TGGTGGAGAA TGTTGATGTG 
TTAACACAAA TGAGGACCAG CTTTGACAAA CCAGACCAGA TGGCTGCACT CTTTAAAAGA 
TTATCATCTG TTGACAGTGT CTTGAAGAGG ATGACAATAA TTGGTGTAAT TTTATCCTTC 
CGATCATTGG CACAAGAAGC ACTTAGAGAT GTCTTATCCT ACCACATTCC TTTTCTTGTA 
AGTTCAATTG AAGATTTTAA GGATCACATT CCAAGGGAAA CTGATATGAA GGTTGCAATG 
AATGTGTATG AGTTATCATC AGCTGCCGGA TTACCTTGTG AGATTGATCC TGCATTGGTC 
GTAGCTCTTT CTTCACAAAA ATCGGAAAAC ATTAGTCCAG AAGAAGAGTA TAAAATTGCC 
TGCCTTCTCA TGGTGTTTGT GGCAGTTTCT TTGCCAACAC TGGCCAGTAA TGTGATGTCT 
CAGTACAGCC CTGCTATAGA AGGGCATTGC AACAACATAC ATTGCTTGGC CAAAGCCATC 
AACCAGATTG CTGCAGCTTT GTTTACAATT CACAAAGGAA GCATTGAAGA CCGTCTTAAA 
GAATTTCTGG CGCTTGCATC CTCCAGTCTA CTGAAAATTG GCCAGGAGAC AGATAAAACT 
ACAACAAGAA ATAGAGAATC CGTTTATTTA CTGCTAGATA TGATTGTACA AGAATCTCCA 
TTCCTTACAA TGGATCTTTT GGAATCTTGT TTTCCTTATG TCTTGCTGAG AAATGCATAC 
CATGCTGTCT ACAAACAAAG TGTTACATCT TCTGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP1 ( XM_016950144.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016805633.1 Pan troglodytes NCK associated protein 1 (NCKAP1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OPc80411
Clone ID Related Accession (Same CDS sequence)	XM_016950144.1 , XM_016950144.2
Accession Version	XM_016950144.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	nck-associated protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006470.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCGCT CAGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGGC ATGTGGAGAC 
CCCAAGGCAA AACCATCCTA TCTTATCGAC AAAAACCTGG AATCTGCTGT GAAATTCATA 
GTCAGAAAAT TCCCTGCTGT AGAAACCCGC AACAACAATC TTGCACAACT ACAGAAAGAA 
AAATCAGAGA TTCTGAAAAA TCTGGCATTA TATTACTTCA CATTTGTAGA TGTTATGGAA 
TTTAAGGACC ATGTTTGTGA ATTGCTGAAT ACTATTGACG TTTGCCAAGT CTTCTTTGAT 
ATTACTGTAA ACTTTGATTT AACAAAGAAC TACTTAGATT TAATTATAAC CTATACAACA 
CTAATGATAC TGCTGTCTCG AATTGAAGAA AGGAAGGCAA TCATTGGATT ATACAACTAT 
GCCCATGAAA TGACTCATGG AGCAAGTGAC AGAGAATACC CACGCCTTGG CCAGATGATT 
GTGGATTATG AAAACCCTTT AAAGAAGATG ATGGAAGAAT TTGTACCCCA TAGCAAGTCT 
CTTTCAGATG CACTAATTTC TCTTCAAATG GTATATCCTC GAAGGAATCT TTCAGCTGAC 
CAGTGGAGAA ATGCCCAGTT ATTGAGCCTC ATCAGTGCGC CTAGTACAAT GCTTAATCCA 
GCGCAGTCCG ACACTATGCC TTGTGAATAC CTCTCTTTGG ATGCAATGGA AAAGTGGATT 
ATCTTTGGCT TTATTTTGTG CCATGGGATC CTAAATACTG ACGCTACAGC ACTGAACCTT 
TGGAAACTAG CTCTTCAAAG TAGCTCTTGC CTCTCTCTCT TTCGGGATGA AGTTTTCCAC 
ATTCACAAAG CTGCAGAAGA CTTATTTGTA AACATACGAG GCTATAATAA ACGTATTAAT 
GACATAAGAG AATGCAAGGA GGCAGCCGTG TCACATGCTG GTTCAATGCA CAGAGAAAGA 
CGCAAGTTTT TAAGATCTGC ACTGAAGGAA TTGGCTACTG TCCTCTCTGA TCAACCTGGA 
TTGCTAGGTC CCAAGGCACT TTTTGTTTTT ATGGCATTAT CCTTTGCCCG TGATGAAATC 
ATCTGGCTAC TTCGTCATGC AGATAACATG CCAAAGAAGA GTGCAGACGA CTTTATAGAT 
AAGCACATTG CTGAATTAAT ATTTTACATG GAAGAACTTA GAGCACATGT GAGGAAATAT 
GGACCTGTAA TGCAGAGGTA TTACGTGCAG TACCTTTCTG GCTTTGATGC TGTTGTCCTC 
AATGAACTCG TGCAGAATCT TTCTGTTTGC CCTGAAGATG AATCAATCAT CATGTCCTCT 
TTTGTTAACA CTATGACTTC CCTAAGTGTA AAACAAGTTG AAGATGGGGA AGTATTTGAT 
TTCAGAGGAA TGAGATTAGA TTGGTTTAGG TTACAGGCAT ATACTAGTGT CTCAAAGGCT 
TCACTTGGCC TTGCAGATCA CAGAGAACTT GGAAAGATGA TGAATACAAT AATTTTTCAT 
ACAAAAATGG TAGATTCCTT GGTGGAAATG TTGGTGGAAA CATCAGATCT CTCCATATTT 
TGTTTTTATA GTCGTGCTTT TGAGAAGATG TTTCAACAGT GTTTGGAGTT ACCCTCTCAA 
TCAAGATACT CAATTGCATT TCCACTACTT TGCACTCATT TTATGAGTTG CACACATGAA 
CTGTGTCCAG AAGAGCGACA TCATATTGGA GATCGCAGTC TTTCCTTATG TAATATGTTC 
CTAGATGAAA TGGCCAAACA AGCTCGAAAT CTCATCACTG ATATTTGCAC AGAACAGTGT 
ACCCTTAGTG ACCAGTTGCT GCCCAAGCAT TGTGCCAAAA CTATCAGTCA AGCAGTGAAT 
AAGAAATCAA AAAAGCAGAC TGGTAAGAAA GGGGAACCTG AAAGGGAGAA ACCAGGTGTT 
GAGAGCATGA GGAAAAACAG GCTGGTTGTG ACCAACCTTG ATAAATTGCA CACTGCACTT 
TCTGAGTTAT GCTTCTCTAT AAATTATGTA CCAAACATGG TGGTATGGGA ACATACCTTT 
ACCCCACGAG AATATTTGAC TTCTCATCTG GAAATACGCT TTACCAAGTC AATTGTTGGG 
ATGACTATGT ATAATCAAGC CACACAGGAA ATTGCAAAAC CTTCAGAACT TCTAACAAGT 
GTAAGAGCAT ACATGACCGT ACTCCAGTCA ATAGAAAACT ATGTGCAGAT TGATATTACA 
AGAGTATTTA ATAATGTGCT TCTTCAACAA ACACAACATT TAGACAGTCA TGGAGAGCCA 
ACCATTACAA GTCTATACAC AAATTGGTAT TTGGAAACTT TGTTACGACA AGTCAGCAAT 
GGCCATATAG CATATTTTCC TGCAATGAAA GCATTTGTGA ACTTACCTAC AGAAAATGAA 
TTAACATTCA ATGCAGAGGA ATATTCTGAC ATATCAGAAA TGAGGTCATT ATCAGAACTA 
CTAGGCCCAT ATGGTATGAA GTTTCTAAGT GAAAGCCTTA TGTGGCATAT TTCGTCACAA 
GTTGCTGAAC TTAAGAAACT TGTGGTGGAG AATGTTGATG TGTTAACACA AATGAGGACC 
AGCTTTGACA AACCAGACCA GATGGCTGCA CTCTTTAAAA GATTATCATC TGTTGACAGT 
GTCTTGAAGA GGATGACAAT AATTGGTGTA ATTTTATCCT TCCGATCATT GGCACAAGAA 
GCACTTAGAG ATGTCTTATC CTACCACATT CCTTTTCTTG TAAGTTCAAT TGAAGATTTT 
AAGGATCACA TTCCAAGGGA AACTGATATG AAGGTTGCAA TGAATGTGTA TGAGTTATCA 
TCAGCTGCCG GATTACCTTG TGAGATTGAT CCTGCATTGG TCGTAGCTCT TTCTTCACAA 
AAATCGGAAA ACATTAGTCC AGAAGAAGAG TATAAAATTG CCTGCCTTCT CATGGTGTTT 
GTGGCAGTTT CTTTGCCAAC ACTGGCCAGT AATGTGATGT CTCAGTACAG CCCTGCTATA 
GAAGGGCATT GCAACAACAT ACATTGCTTG GCCAAAGCCA TCAACCAGAT TGCTGCAGCT 
TTGTTTACAA TTCACAAAGG AAGCATTGAA GACCGTCTTA AAGAATTTCT GGCGCTTGCA 
TCCTCCAGTC TACTGAAAAT TGGCCAGGAG ACAGATAAAA CTACAACAAG AAATAGAGAA 
TCCGTTTATT TACTGCTAGA TATGATTGTA CAAGAATCTC CATTCCTTAC AATGGATCTT 
TTGGAATCTT GTTTTCCTTA TGTCTTGCTG AGAAATGCAT ACCATGCTGT CTACAAACAA 
AGTGTTACAT CTTCTGCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016805633.1
CDS	1..3381
Translation	MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNYLDLIITYTTLMILLSRIEERKAIIGLYNYAHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNTDATALNLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSHAGSMHRERRKFLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLGLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYVPNMVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA

Target ORF information:

RefSeq Version	XM_016950144.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes NCK associated protein 1 (NCKAP1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016950144.1

ORF Insert Sequence:

ATGTCGCGCT CAGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGGC ATGTGGAGAC 
CCCAAGGCAA AACCATCCTA TCTTATCGAC AAAAACCTGG AATCTGCTGT GAAATTCATA 
GTCAGAAAAT TCCCTGCTGT AGAAACCCGC AACAACAATC TTGCACAACT ACAGAAAGAA 
AAATCAGAGA TTCTGAAAAA TCTGGCATTA TATTACTTCA CATTTGTAGA TGTTATGGAA 
TTTAAGGACC ATGTTTGTGA ATTGCTGAAT ACTATTGACG TTTGCCAAGT CTTCTTTGAT 
ATTACTGTAA ACTTTGATTT AACAAAGAAC TACTTAGATT TAATTATAAC CTATACAACA 
CTAATGATAC TGCTGTCTCG AATTGAAGAA AGGAAGGCAA TCATTGGATT ATACAACTAT 
GCCCATGAAA TGACTCATGG AGCAAGTGAC AGAGAATACC CACGCCTTGG CCAGATGATT 
GTGGATTATG AAAACCCTTT AAAGAAGATG ATGGAAGAAT TTGTACCCCA TAGCAAGTCT 
CTTTCAGATG CACTAATTTC TCTTCAAATG GTATATCCTC GAAGGAATCT TTCAGCTGAC 
CAGTGGAGAA ATGCCCAGTT ATTGAGCCTC ATCAGTGCGC CTAGTACAAT GCTTAATCCA 
GCGCAGTCCG ACACTATGCC TTGTGAATAC CTCTCTTTGG ATGCAATGGA AAAGTGGATT 
ATCTTTGGCT TTATTTTGTG CCATGGGATC CTAAATACTG ACGCTACAGC ACTGAACCTT 
TGGAAACTAG CTCTTCAAAG TAGCTCTTGC CTCTCTCTCT TTCGGGATGA AGTTTTCCAC 
ATTCACAAAG CTGCAGAAGA CTTATTTGTA AACATACGAG GCTATAATAA ACGTATTAAT 
GACATAAGAG AATGCAAGGA GGCAGCCGTG TCACATGCTG GTTCAATGCA CAGAGAAAGA 
CGCAAGTTTT TAAGATCTGC ACTGAAGGAA TTGGCTACTG TCCTCTCTGA TCAACCTGGA 
TTGCTAGGTC CCAAGGCACT TTTTGTTTTT ATGGCATTAT CCTTTGCCCG TGATGAAATC 
ATCTGGCTAC TTCGTCATGC AGATAACATG CCAAAGAAGA GTGCAGACGA CTTTATAGAT 
AAGCACATTG CTGAATTAAT ATTTTACATG GAAGAACTTA GAGCACATGT GAGGAAATAT 
GGACCTGTAA TGCAGAGGTA TTACGTGCAG TACCTTTCTG GCTTTGATGC TGTTGTCCTC 
AATGAACTCG TGCAGAATCT TTCTGTTTGC CCTGAAGATG AATCAATCAT CATGTCCTCT 
TTTGTTAACA CTATGACTTC CCTAAGTGTA AAACAAGTTG AAGATGGGGA AGTATTTGAT 
TTCAGAGGAA TGAGATTAGA TTGGTTTAGG TTACAGGCAT ATACTAGTGT CTCAAAGGCT 
TCACTTGGCC TTGCAGATCA CAGAGAACTT GGAAAGATGA TGAATACAAT AATTTTTCAT 
ACAAAAATGG TAGATTCCTT GGTGGAAATG TTGGTGGAAA CATCAGATCT CTCCATATTT 
TGTTTTTATA GTCGTGCTTT TGAGAAGATG TTTCAACAGT GTTTGGAGTT ACCCTCTCAA 
TCAAGATACT CAATTGCATT TCCACTACTT TGCACTCATT TTATGAGTTG CACACATGAA 
CTGTGTCCAG AAGAGCGACA TCATATTGGA GATCGCAGTC TTTCCTTATG TAATATGTTC 
CTAGATGAAA TGGCCAAACA AGCTCGAAAT CTCATCACTG ATATTTGCAC AGAACAGTGT 
ACCCTTAGTG ACCAGTTGCT GCCCAAGCAT TGTGCCAAAA CTATCAGTCA AGCAGTGAAT 
AAGAAATCAA AAAAGCAGAC TGGTAAGAAA GGGGAACCTG AAAGGGAGAA ACCAGGTGTT 
GAGAGCATGA GGAAAAACAG GCTGGTTGTG ACCAACCTTG ATAAATTGCA CACTGCACTT 
TCTGAGTTAT GCTTCTCTAT AAATTATGTA CCAAACATGG TGGTATGGGA ACATACCTTT 
ACCCCACGAG AATATTTGAC TTCTCATCTG GAAATACGCT TTACCAAGTC AATTGTTGGG 
ATGACTATGT ATAATCAAGC CACACAGGAA ATTGCAAAAC CTTCAGAACT TCTAACAAGT 
GTAAGAGCAT ACATGACCGT ACTCCAGTCA ATAGAAAACT ATGTGCAGAT TGATATTACA 
AGAGTATTTA ATAATGTGCT TCTTCAACAA ACACAACATT TAGACAGTCA TGGAGAGCCA 
ACCATTACAA GTCTATACAC AAATTGGTAT TTGGAAACTT TGTTACGACA AGTCAGCAAT 
GGCCATATAG CATATTTTCC TGCAATGAAA GCATTTGTGA ACTTACCTAC AGAAAATGAA 
TTAACATTCA ATGCAGAGGA ATATTCTGAC ATATCAGAAA TGAGGTCATT ATCAGAACTA 
CTAGGCCCAT ATGGTATGAA GTTTCTAAGT GAAAGCCTTA TGTGGCATAT TTCGTCACAA 
GTTGCTGAAC TTAAGAAACT TGTGGTGGAG AATGTTGATG TGTTAACACA AATGAGGACC 
AGCTTTGACA AACCAGACCA GATGGCTGCA CTCTTTAAAA GATTATCATC TGTTGACAGT 
GTCTTGAAGA GGATGACAAT AATTGGTGTA ATTTTATCCT TCCGATCATT GGCACAAGAA 
GCACTTAGAG ATGTCTTATC CTACCACATT CCTTTTCTTG TAAGTTCAAT TGAAGATTTT 
AAGGATCACA TTCCAAGGGA AACTGATATG AAGGTTGCAA TGAATGTGTA TGAGTTATCA 
TCAGCTGCCG GATTACCTTG TGAGATTGAT CCTGCATTGG TCGTAGCTCT TTCTTCACAA 
AAATCGGAAA ACATTAGTCC AGAAGAAGAG TATAAAATTG CCTGCCTTCT CATGGTGTTT 
GTGGCAGTTT CTTTGCCAAC ACTGGCCAGT AATGTGATGT CTCAGTACAG CCCTGCTATA 
GAAGGGCATT GCAACAACAT ACATTGCTTG GCCAAAGCCA TCAACCAGAT TGCTGCAGCT 
TTGTTTACAA TTCACAAAGG AAGCATTGAA GACCGTCTTA AAGAATTTCT GGCGCTTGCA 
TCCTCCAGTC TACTGAAAAT TGGCCAGGAG ACAGATAAAA CTACAACAAG AAATAGAGAA 
TCCGTTTATT TACTGCTAGA TATGATTGTA CAAGAATCTC CATTCCTTAC AATGGATCTT 
TTGGAATCTT GTTTTCCTTA TGTCTTGCTG AGAAATGCAT ACCATGCTGT CTACAAACAA 
AGTGTTACAT CTTCTGCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP1 ( XM_016950144.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016805633.1 Pan troglodytes NCK associated protein 1 (NCKAP1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OPc80411
Clone ID Related Accession (Same CDS sequence)	XM_016950144.1 , XM_016950144.2
Accession Version	XM_016950144.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	nck-associated protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036881.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016950144.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCGCT CAGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGGC ATGTGGAGAC 
CCCAAGGCAA AACCATCCTA TCTTATCGAC AAAAACCTGG AATCTGCTGT GAAATTCATA 
GTCAGAAAAT TCCCTGCTGT AGAAACCCGC AACAACAATC TTGCACAACT ACAGAAAGAA 
AAATCAGAGA TTCTGAAAAA TCTGGCATTA TATTACTTCA CATTTGTAGA TGTTATGGAA 
TTTAAGGACC ATGTTTGTGA ATTGCTGAAT ACTATTGACG TTTGCCAAGT CTTCTTTGAT 
ATTACTGTAA ACTTTGATTT AACAAAGAAC TACTTAGATT TAATTATAAC CTATACAACA 
CTAATGATAC TGCTGTCTCG AATTGAAGAA AGGAAGGCAA TCATTGGATT ATACAACTAT 
GCCCATGAAA TGACTCATGG AGCAAGTGAC AGAGAATACC CACGCCTTGG CCAGATGATT 
GTGGATTATG AAAACCCTTT AAAGAAGATG ATGGAAGAAT TTGTACCCCA TAGCAAGTCT 
CTTTCAGATG CACTAATTTC TCTTCAAATG GTATATCCTC GAAGGAATCT TTCAGCTGAC 
CAGTGGAGAA ATGCCCAGTT ATTGAGCCTC ATCAGTGCGC CTAGTACAAT GCTTAATCCA 
GCGCAGTCCG ACACTATGCC TTGTGAATAC CTCTCTTTGG ATGCAATGGA AAAGTGGATT 
ATCTTTGGCT TTATTTTGTG CCATGGGATC CTAAATACTG ACGCTACAGC ACTGAACCTT 
TGGAAACTAG CTCTTCAAAG TAGCTCTTGC CTCTCTCTCT TTCGGGATGA AGTTTTCCAC 
ATTCACAAAG CTGCAGAAGA CTTATTTGTA AACATACGAG GCTATAATAA ACGTATTAAT 
GACATAAGAG AATGCAAGGA GGCAGCCGTG TCACATGCTG GTTCAATGCA CAGAGAAAGA 
CGCAAGTTTT TAAGATCTGC ACTGAAGGAA TTGGCTACTG TCCTCTCTGA TCAACCTGGA 
TTGCTAGGTC CCAAGGCACT TTTTGTTTTT ATGGCATTAT CCTTTGCCCG TGATGAAATC 
ATCTGGCTAC TTCGTCATGC AGATAACATG CCAAAGAAGA GTGCAGACGA CTTTATAGAT 
AAGCACATTG CTGAATTAAT ATTTTACATG GAAGAACTTA GAGCACATGT GAGGAAATAT 
GGACCTGTAA TGCAGAGGTA TTACGTGCAG TACCTTTCTG GCTTTGATGC TGTTGTCCTC 
AATGAACTCG TGCAGAATCT TTCTGTTTGC CCTGAAGATG AATCAATCAT CATGTCCTCT 
TTTGTTAACA CTATGACTTC CCTAAGTGTA AAACAAGTTG AAGATGGGGA AGTATTTGAT 
TTCAGAGGAA TGAGATTAGA TTGGTTTAGG TTACAGGCAT ATACTAGTGT CTCAAAGGCT 
TCACTTGGCC TTGCAGATCA CAGAGAACTT GGAAAGATGA TGAATACAAT AATTTTTCAT 
ACAAAAATGG TAGATTCCTT GGTGGAAATG TTGGTGGAAA CATCAGATCT CTCCATATTT 
TGTTTTTATA GTCGTGCTTT TGAGAAGATG TTTCAACAGT GTTTGGAGTT ACCCTCTCAA 
TCAAGATACT CAATTGCATT TCCACTACTT TGCACTCATT TTATGAGTTG CACACATGAA 
CTGTGTCCAG AAGAGCGACA TCATATTGGA GATCGCAGTC TTTCCTTATG TAATATGTTC 
CTAGATGAAA TGGCCAAACA AGCTCGAAAT CTCATCACTG ATATTTGCAC AGAACAGTGT 
ACCCTTAGTG ACCAGTTGCT GCCCAAGCAT TGTGCCAAAA CTATCAGTCA AGCAGTGAAT 
AAGAAATCAA AAAAGCAGAC TGGTAAGAAA GGGGAACCTG AAAGGGAGAA ACCAGGTGTT 
GAGAGCATGA GGAAAAACAG GCTGGTTGTG ACCAACCTTG ATAAATTGCA CACTGCACTT 
TCTGAGTTAT GCTTCTCTAT AAATTATGTA CCAAACATGG TGGTATGGGA ACATACCTTT 
ACCCCACGAG AATATTTGAC TTCTCATCTG GAAATACGCT TTACCAAGTC AATTGTTGGG 
ATGACTATGT ATAATCAAGC CACACAGGAA ATTGCAAAAC CTTCAGAACT TCTAACAAGT 
GTAAGAGCAT ACATGACCGT ACTCCAGTCA ATAGAAAACT ATGTGCAGAT TGATATTACA 
AGAGTATTTA ATAATGTGCT TCTTCAACAA ACACAACATT TAGACAGTCA TGGAGAGCCA 
ACCATTACAA GTCTATACAC AAATTGGTAT TTGGAAACTT TGTTACGACA AGTCAGCAAT 
GGCCATATAG CATATTTTCC TGCAATGAAA GCATTTGTGA ACTTACCTAC AGAAAATGAA 
TTAACATTCA ATGCAGAGGA ATATTCTGAC ATATCAGAAA TGAGGTCATT ATCAGAACTA 
CTAGGCCCAT ATGGTATGAA GTTTCTAAGT GAAAGCCTTA TGTGGCATAT TTCGTCACAA 
GTTGCTGAAC TTAAGAAACT TGTGGTGGAG AATGTTGATG TGTTAACACA AATGAGGACC 
AGCTTTGACA AACCAGACCA GATGGCTGCA CTCTTTAAAA GATTATCATC TGTTGACAGT 
GTCTTGAAGA GGATGACAAT AATTGGTGTA ATTTTATCCT TCCGATCATT GGCACAAGAA 
GCACTTAGAG ATGTCTTATC CTACCACATT CCTTTTCTTG TAAGTTCAAT TGAAGATTTT 
AAGGATCACA TTCCAAGGGA AACTGATATG AAGGTTGCAA TGAATGTGTA TGAGTTATCA 
TCAGCTGCCG GATTACCTTG TGAGATTGAT CCTGCATTGG TCGTAGCTCT TTCTTCACAA 
AAATCGGAAA ACATTAGTCC AGAAGAAGAG TATAAAATTG CCTGCCTTCT CATGGTGTTT 
GTGGCAGTTT CTTTGCCAAC ACTGGCCAGT AATGTGATGT CTCAGTACAG CCCTGCTATA 
GAAGGGCATT GCAACAACAT ACATTGCTTG GCCAAAGCCA TCAACCAGAT TGCTGCAGCT 
TTGTTTACAA TTCACAAAGG AAGCATTGAA GACCGTCTTA AAGAATTTCT GGCGCTTGCA 
TCCTCCAGTC TACTGAAAAT TGGCCAGGAG ACAGATAAAA CTACAACAAG AAATAGAGAA 
TCCGTTTATT TACTGCTAGA TATGATTGTA CAAGAATCTC CATTCCTTAC AATGGATCTT 
TTGGAATCTT GTTTTCCTTA TGTCTTGCTG AGAAATGCAT ACCATGCTGT CTACAAACAA 
AGTGTTACAT CTTCTGCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016805633.1
CDS	1..3381
Translation	MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNYLDLIITYTTLMILLSRIEERKAIIGLYNYAHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNTDATALNLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSHAGSMHRERRKFLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLGLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYVPNMVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA

Target ORF information:

RefSeq Version	XM_016950144.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes NCK associated protein 1 (NCKAP1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016950144.2

ORF Insert Sequence:

ATGTCGCGCT CAGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGGC ATGTGGAGAC 
CCCAAGGCAA AACCATCCTA TCTTATCGAC AAAAACCTGG AATCTGCTGT GAAATTCATA 
GTCAGAAAAT TCCCTGCTGT AGAAACCCGC AACAACAATC TTGCACAACT ACAGAAAGAA 
AAATCAGAGA TTCTGAAAAA TCTGGCATTA TATTACTTCA CATTTGTAGA TGTTATGGAA 
TTTAAGGACC ATGTTTGTGA ATTGCTGAAT ACTATTGACG TTTGCCAAGT CTTCTTTGAT 
ATTACTGTAA ACTTTGATTT AACAAAGAAC TACTTAGATT TAATTATAAC CTATACAACA 
CTAATGATAC TGCTGTCTCG AATTGAAGAA AGGAAGGCAA TCATTGGATT ATACAACTAT 
GCCCATGAAA TGACTCATGG AGCAAGTGAC AGAGAATACC CACGCCTTGG CCAGATGATT 
GTGGATTATG AAAACCCTTT AAAGAAGATG ATGGAAGAAT TTGTACCCCA TAGCAAGTCT 
CTTTCAGATG CACTAATTTC TCTTCAAATG GTATATCCTC GAAGGAATCT TTCAGCTGAC 
CAGTGGAGAA ATGCCCAGTT ATTGAGCCTC ATCAGTGCGC CTAGTACAAT GCTTAATCCA 
GCGCAGTCCG ACACTATGCC TTGTGAATAC CTCTCTTTGG ATGCAATGGA AAAGTGGATT 
ATCTTTGGCT TTATTTTGTG CCATGGGATC CTAAATACTG ACGCTACAGC ACTGAACCTT 
TGGAAACTAG CTCTTCAAAG TAGCTCTTGC CTCTCTCTCT TTCGGGATGA AGTTTTCCAC 
ATTCACAAAG CTGCAGAAGA CTTATTTGTA AACATACGAG GCTATAATAA ACGTATTAAT 
GACATAAGAG AATGCAAGGA GGCAGCCGTG TCACATGCTG GTTCAATGCA CAGAGAAAGA 
CGCAAGTTTT TAAGATCTGC ACTGAAGGAA TTGGCTACTG TCCTCTCTGA TCAACCTGGA 
TTGCTAGGTC CCAAGGCACT TTTTGTTTTT ATGGCATTAT CCTTTGCCCG TGATGAAATC 
ATCTGGCTAC TTCGTCATGC AGATAACATG CCAAAGAAGA GTGCAGACGA CTTTATAGAT 
AAGCACATTG CTGAATTAAT ATTTTACATG GAAGAACTTA GAGCACATGT GAGGAAATAT 
GGACCTGTAA TGCAGAGGTA TTACGTGCAG TACCTTTCTG GCTTTGATGC TGTTGTCCTC 
AATGAACTCG TGCAGAATCT TTCTGTTTGC CCTGAAGATG AATCAATCAT CATGTCCTCT 
TTTGTTAACA CTATGACTTC CCTAAGTGTA AAACAAGTTG AAGATGGGGA AGTATTTGAT 
TTCAGAGGAA TGAGATTAGA TTGGTTTAGG TTACAGGCAT ATACTAGTGT CTCAAAGGCT 
TCACTTGGCC TTGCAGATCA CAGAGAACTT GGAAAGATGA TGAATACAAT AATTTTTCAT 
ACAAAAATGG TAGATTCCTT GGTGGAAATG TTGGTGGAAA CATCAGATCT CTCCATATTT 
TGTTTTTATA GTCGTGCTTT TGAGAAGATG TTTCAACAGT GTTTGGAGTT ACCCTCTCAA 
TCAAGATACT CAATTGCATT TCCACTACTT TGCACTCATT TTATGAGTTG CACACATGAA 
CTGTGTCCAG AAGAGCGACA TCATATTGGA GATCGCAGTC TTTCCTTATG TAATATGTTC 
CTAGATGAAA TGGCCAAACA AGCTCGAAAT CTCATCACTG ATATTTGCAC AGAACAGTGT 
ACCCTTAGTG ACCAGTTGCT GCCCAAGCAT TGTGCCAAAA CTATCAGTCA AGCAGTGAAT 
AAGAAATCAA AAAAGCAGAC TGGTAAGAAA GGGGAACCTG AAAGGGAGAA ACCAGGTGTT 
GAGAGCATGA GGAAAAACAG GCTGGTTGTG ACCAACCTTG ATAAATTGCA CACTGCACTT 
TCTGAGTTAT GCTTCTCTAT AAATTATGTA CCAAACATGG TGGTATGGGA ACATACCTTT 
ACCCCACGAG AATATTTGAC TTCTCATCTG GAAATACGCT TTACCAAGTC AATTGTTGGG 
ATGACTATGT ATAATCAAGC CACACAGGAA ATTGCAAAAC CTTCAGAACT TCTAACAAGT 
GTAAGAGCAT ACATGACCGT ACTCCAGTCA ATAGAAAACT ATGTGCAGAT TGATATTACA 
AGAGTATTTA ATAATGTGCT TCTTCAACAA ACACAACATT TAGACAGTCA TGGAGAGCCA 
ACCATTACAA GTCTATACAC AAATTGGTAT TTGGAAACTT TGTTACGACA AGTCAGCAAT 
GGCCATATAG CATATTTTCC TGCAATGAAA GCATTTGTGA ACTTACCTAC AGAAAATGAA 
TTAACATTCA ATGCAGAGGA ATATTCTGAC ATATCAGAAA TGAGGTCATT ATCAGAACTA 
CTAGGCCCAT ATGGTATGAA GTTTCTAAGT GAAAGCCTTA TGTGGCATAT TTCGTCACAA 
GTTGCTGAAC TTAAGAAACT TGTGGTGGAG AATGTTGATG TGTTAACACA AATGAGGACC 
AGCTTTGACA AACCAGACCA GATGGCTGCA CTCTTTAAAA GATTATCATC TGTTGACAGT 
GTCTTGAAGA GGATGACAAT AATTGGTGTA ATTTTATCCT TCCGATCATT GGCACAAGAA 
GCACTTAGAG ATGTCTTATC CTACCACATT CCTTTTCTTG TAAGTTCAAT TGAAGATTTT 
AAGGATCACA TTCCAAGGGA AACTGATATG AAGGTTGCAA TGAATGTGTA TGAGTTATCA 
TCAGCTGCCG GATTACCTTG TGAGATTGAT CCTGCATTGG TCGTAGCTCT TTCTTCACAA 
AAATCGGAAA ACATTAGTCC AGAAGAAGAG TATAAAATTG CCTGCCTTCT CATGGTGTTT 
GTGGCAGTTT CTTTGCCAAC ACTGGCCAGT AATGTGATGT CTCAGTACAG CCCTGCTATA 
GAAGGGCATT GCAACAACAT ACATTGCTTG GCCAAAGCCA TCAACCAGAT TGCTGCAGCT 
TTGTTTACAA TTCACAAAGG AAGCATTGAA GACCGTCTTA AAGAATTTCT GGCGCTTGCA 
TCCTCCAGTC TACTGAAAAT TGGCCAGGAG ACAGATAAAA CTACAACAAG AAATAGAGAA 
TCCGTTTATT TACTGCTAGA TATGATTGTA CAAGAATCTC CATTCCTTAC AATGGATCTT 
TTGGAATCTT GTTTTCCTTA TGTCTTGCTG AGAAATGCAT ACCATGCTGT CTACAAACAA 
AGTGTTACAT CTTCTGCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP1 ( XM_003309356.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_003309404.1 Pan troglodytes NCK associated protein 1 (NCKAP1), transcript variant X3, mRNA.

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CloneID	OPc05680
Clone ID Related Accession (Same CDS sequence)	XM_003309356.3 , XM_003309356.4
Accession Version	XM_003309356.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	nck-associated protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006470.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 7, 2014 this sequence version replaced XM_003309356.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCGCT CAGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGGC ATGTGGAGAC 
CCCAAGGCAA AACCATCCTA TCTTATCGAC AAAAACCTGG AATCTGCTGT GAAATTCATA 
GTCAGAAAAT TCCCTGCTGT AGAAACCCGC AACAACAATC AACAGCTTGC ACAACTACAG 
AAAGAAAAAT CAGAGATTCT GAAAAATCTG GCATTATATT ACTTCACATT TGTAGATGTT 
ATGGAATTTA AGGACCATGT TTGTGAATTG CTGAATACTA TTGACGTTTG CCAAGTCTTC 
TTTGATATTA CTGTAAACTT TGATTTAACA AAGAACTACT TAGATTTAAT TATAACCTAT 
ACAACACTAA TGATACTGCT GTCTCGAATT GAAGAAAGGA AGGCAATCAT TGGATTATAC 
AACTATGCCC ATGAAATGAC TCATGGAGCA AGTGACAGAG AATACCCACG CCTTGGCCAG 
ATGATTGTGG ATTATGAAAA CCCTTTAAAG AAGATGATGG AAGAATTTGT ACCCCATAGC 
AAGTCTCTTT CAGATGCACT AATTTCTCTT CAAATGGTAT ATCCTCGAAG GAATCTTTCA 
GCTGACCAGT GGAGAAATGC CCAGTTATTG AGCCTCATCA GTGCGCCTAG TACAATGCTT 
AATCCAGCGC AGTCCGACAC TATGCCTTGT GAATACCTCT CTTTGGATGC AATGGAAAAG 
TGGATTATCT TTGGCTTTAT TTTGTGCCAT GGGATCCTAA ATACTGACGC TACAGCACTG 
AACCTTTGGA AACTAGCTCT TCAAAGTAGC TCTTGCCTCT CTCTCTTTCG GGATGAAGTT 
TTCCACATTC ACAAAGCTGC AGAAGACTTA TTTGTAAACA TACGAGGCTA TAATAAACGT 
ATTAATGACA TAAGAGAATG CAAGGAGGCA GCCGTGTCAC ATGCTGGTTC AATGCACAGA 
GAAAGACGCA AGTTTTTAAG ATCTGCACTG AAGGAATTGG CTACTGTCCT CTCTGATCAA 
CCTGGATTGC TAGGTCCCAA GGCACTTTTT GTTTTTATGG CATTATCCTT TGCCCGTGAT 
GAAATCATCT GGCTACTTCG TCATGCAGAT AACATGCCAA AGAAGAGTGC AGACGACTTT 
ATAGATAAGC ACATTGCTGA ATTAATATTT TACATGGAAG AACTTAGAGC ACATGTGAGG 
AAATATGGAC CTGTAATGCA GAGGTATTAC GTGCAGTACC TTTCTGGCTT TGATGCTGTT 
GTCCTCAATG AACTCGTGCA GAATCTTTCT GTTTGCCCTG AAGATGAATC AATCATCATG 
TCCTCTTTTG TTAACACTAT GACTTCCCTA AGTGTAAAAC AAGTTGAAGA TGGGGAAGTA 
TTTGATTTCA GAGGAATGAG ATTAGATTGG TTTAGGTTAC AGGCATATAC TAGTGTCTCA 
AAGGCTTCAC TTGGCCTTGC AGATCACAGA GAACTTGGAA AGATGATGAA TACAATAATT 
TTTCATACAA AAATGGTAGA TTCCTTGGTG GAAATGTTGG TGGAAACATC AGATCTCTCC 
ATATTTTGTT TTTATAGTCG TGCTTTTGAG AAGATGTTTC AACAGTGTTT GGAGTTACCC 
TCTCAATCAA GATACTCAAT TGCATTTCCA CTACTTTGCA CTCATTTTAT GAGTTGCACA 
CATGAACTGT GTCCAGAAGA GCGACATCAT ATTGGAGATC GCAGTCTTTC CTTATGTAAT 
ATGTTCCTAG ATGAAATGGC CAAACAAGCT CGAAATCTCA TCACTGATAT TTGCACAGAA 
CAGTGTACCC TTAGTGACCA GTTGCTGCCC AAGCATTGTG CCAAAACTAT CAGTCAAGCA 
GTGAATAAGA AATCAAAAAA GCAGACTGGT AAGAAAGGGG AACCTGAAAG GGAGAAACCA 
GGTGTTGAGA GCATGAGGAA AAACAGGCTG GTTGTGACCA ACCTTGATAA ATTGCACACT 
GCACTTTCTG AGTTATGCTT CTCTATAAAT TATGTACCAA ACATGGTGGT ATGGGAACAT 
ACCTTTACCC CACGAGAATA TTTGACTTCT CATCTGGAAA TACGCTTTAC CAAGTCAATT 
GTTGGGATGA CTATGTATAA TCAAGCCACA CAGGAAATTG CAAAACCTTC AGAACTTCTA 
ACAAGTGTAA GAGCATACAT GACCGTACTC CAGTCAATAG AAAACTATGT GCAGATTGAT 
ATTACAAGAG TATTTAATAA TGTGCTTCTT CAACAAACAC AACATTTAGA CAGTCATGGA 
GAGCCAACCA TTACAAGTCT ATACACAAAT TGGTATTTGG AAACTTTGTT ACGACAAGTC 
AGCAATGGCC ATATAGCATA TTTTCCTGCA ATGAAAGCAT TTGTGAACTT ACCTACAGAA 
AATGAATTAA CATTCAATGC AGAGGAATAT TCTGACATAT CAGAAATGAG GTCATTATCA 
GAACTACTAG GCCCATATGG TATGAAGTTT CTAAGTGAAA GCCTTATGTG GCATATTTCG 
TCACAAGTTG CTGAACTTAA GAAACTTGTG GTGGAGAATG TTGATGTGTT AACACAAATG 
AGGACCAGCT TTGACAAACC AGACCAGATG GCTGCACTCT TTAAAAGATT ATCATCTGTT 
GACAGTGTCT TGAAGAGGAT GACAATAATT GGTGTAATTT TATCCTTCCG ATCATTGGCA 
CAAGAAGCAC TTAGAGATGT CTTATCCTAC CACATTCCTT TTCTTGTAAG TTCAATTGAA 
GATTTTAAGG ATCACATTCC AAGGGAAACT GATATGAAGG TTGCAATGAA TGTGTATGAG 
TTATCATCAG CTGCCGGATT ACCTTGTGAG ATTGATCCTG CATTGGTCGT AGCTCTTTCT 
TCACAAAAAT CGGAAAACAT TAGTCCAGAA GAAGAGTATA AAATTGCCTG CCTTCTCATG 
GTGTTTGTGG CAGTTTCTTT GCCAACACTG GCCAGTAATG TGATGTCTCA GTACAGCCCT 
GCTATAGAAG GGCATTGCAA CAACATACAT TGCTTGGCCA AAGCCATCAA CCAGATTGCT 
GCAGCTTTGT TTACAATTCA CAAAGGAAGC ATTGAAGACC GTCTTAAAGA ATTTCTGGCG 
CTTGCATCCT CCAGTCTACT GAAAATTGGC CAGGAGACAG ATAAAACTAC AACAAGAAAT 
AGAGAATCCG TTTATTTACT GCTAGATATG ATTGTACAAG AATCTCCATT CCTTACAATG 
GATCTTTTGG AATCTTGTTT TCCTTATGTC TTGCTGAGAA ATGCATACCA TGCTGTCTAC 
AAACAAAGTG TTACATCTTC TGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003309404.1
CDS	757..4143
Translation	MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNYLDLIITYTTLMILLSRIEERKAIIGLYNYAHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNTDATALNLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSHAGSMHRERRKFLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLGLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYVPNMVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA

Target ORF information:

RefSeq Version	XM_003309356.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes NCK associated protein 1 (NCKAP1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003309356.3

ORF Insert Sequence:

ATGTCGCGCT CAGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGGC ATGTGGAGAC 
CCCAAGGCAA AACCATCCTA TCTTATCGAC AAAAACCTGG AATCTGCTGT GAAATTCATA 
GTCAGAAAAT TCCCTGCTGT AGAAACCCGC AACAACAATC AACAGCTTGC ACAACTACAG 
AAAGAAAAAT CAGAGATTCT GAAAAATCTG GCATTATATT ACTTCACATT TGTAGATGTT 
ATGGAATTTA AGGACCATGT TTGTGAATTG CTGAATACTA TTGACGTTTG CCAAGTCTTC 
TTTGATATTA CTGTAAACTT TGATTTAACA AAGAACTACT TAGATTTAAT TATAACCTAT 
ACAACACTAA TGATACTGCT GTCTCGAATT GAAGAAAGGA AGGCAATCAT TGGATTATAC 
AACTATGCCC ATGAAATGAC TCATGGAGCA AGTGACAGAG AATACCCACG CCTTGGCCAG 
ATGATTGTGG ATTATGAAAA CCCTTTAAAG AAGATGATGG AAGAATTTGT ACCCCATAGC 
AAGTCTCTTT CAGATGCACT AATTTCTCTT CAAATGGTAT ATCCTCGAAG GAATCTTTCA 
GCTGACCAGT GGAGAAATGC CCAGTTATTG AGCCTCATCA GTGCGCCTAG TACAATGCTT 
AATCCAGCGC AGTCCGACAC TATGCCTTGT GAATACCTCT CTTTGGATGC AATGGAAAAG 
TGGATTATCT TTGGCTTTAT TTTGTGCCAT GGGATCCTAA ATACTGACGC TACAGCACTG 
AACCTTTGGA AACTAGCTCT TCAAAGTAGC TCTTGCCTCT CTCTCTTTCG GGATGAAGTT 
TTCCACATTC ACAAAGCTGC AGAAGACTTA TTTGTAAACA TACGAGGCTA TAATAAACGT 
ATTAATGACA TAAGAGAATG CAAGGAGGCA GCCGTGTCAC ATGCTGGTTC AATGCACAGA 
GAAAGACGCA AGTTTTTAAG ATCTGCACTG AAGGAATTGG CTACTGTCCT CTCTGATCAA 
CCTGGATTGC TAGGTCCCAA GGCACTTTTT GTTTTTATGG CATTATCCTT TGCCCGTGAT 
GAAATCATCT GGCTACTTCG TCATGCAGAT AACATGCCAA AGAAGAGTGC AGACGACTTT 
ATAGATAAGC ACATTGCTGA ATTAATATTT TACATGGAAG AACTTAGAGC ACATGTGAGG 
AAATATGGAC CTGTAATGCA GAGGTATTAC GTGCAGTACC TTTCTGGCTT TGATGCTGTT 
GTCCTCAATG AACTCGTGCA GAATCTTTCT GTTTGCCCTG AAGATGAATC AATCATCATG 
TCCTCTTTTG TTAACACTAT GACTTCCCTA AGTGTAAAAC AAGTTGAAGA TGGGGAAGTA 
TTTGATTTCA GAGGAATGAG ATTAGATTGG TTTAGGTTAC AGGCATATAC TAGTGTCTCA 
AAGGCTTCAC TTGGCCTTGC AGATCACAGA GAACTTGGAA AGATGATGAA TACAATAATT 
TTTCATACAA AAATGGTAGA TTCCTTGGTG GAAATGTTGG TGGAAACATC AGATCTCTCC 
ATATTTTGTT TTTATAGTCG TGCTTTTGAG AAGATGTTTC AACAGTGTTT GGAGTTACCC 
TCTCAATCAA GATACTCAAT TGCATTTCCA CTACTTTGCA CTCATTTTAT GAGTTGCACA 
CATGAACTGT GTCCAGAAGA GCGACATCAT ATTGGAGATC GCAGTCTTTC CTTATGTAAT 
ATGTTCCTAG ATGAAATGGC CAAACAAGCT CGAAATCTCA TCACTGATAT TTGCACAGAA 
CAGTGTACCC TTAGTGACCA GTTGCTGCCC AAGCATTGTG CCAAAACTAT CAGTCAAGCA 
GTGAATAAGA AATCAAAAAA GCAGACTGGT AAGAAAGGGG AACCTGAAAG GGAGAAACCA 
GGTGTTGAGA GCATGAGGAA AAACAGGCTG GTTGTGACCA ACCTTGATAA ATTGCACACT 
GCACTTTCTG AGTTATGCTT CTCTATAAAT TATGTACCAA ACATGGTGGT ATGGGAACAT 
ACCTTTACCC CACGAGAATA TTTGACTTCT CATCTGGAAA TACGCTTTAC CAAGTCAATT 
GTTGGGATGA CTATGTATAA TCAAGCCACA CAGGAAATTG CAAAACCTTC AGAACTTCTA 
ACAAGTGTAA GAGCATACAT GACCGTACTC CAGTCAATAG AAAACTATGT GCAGATTGAT 
ATTACAAGAG TATTTAATAA TGTGCTTCTT CAACAAACAC AACATTTAGA CAGTCATGGA 
GAGCCAACCA TTACAAGTCT ATACACAAAT TGGTATTTGG AAACTTTGTT ACGACAAGTC 
AGCAATGGCC ATATAGCATA TTTTCCTGCA ATGAAAGCAT TTGTGAACTT ACCTACAGAA 
AATGAATTAA CATTCAATGC AGAGGAATAT TCTGACATAT CAGAAATGAG GTCATTATCA 
GAACTACTAG GCCCATATGG TATGAAGTTT CTAAGTGAAA GCCTTATGTG GCATATTTCG 
TCACAAGTTG CTGAACTTAA GAAACTTGTG GTGGAGAATG TTGATGTGTT AACACAAATG 
AGGACCAGCT TTGACAAACC AGACCAGATG GCTGCACTCT TTAAAAGATT ATCATCTGTT 
GACAGTGTCT TGAAGAGGAT GACAATAATT GGTGTAATTT TATCCTTCCG ATCATTGGCA 
CAAGAAGCAC TTAGAGATGT CTTATCCTAC CACATTCCTT TTCTTGTAAG TTCAATTGAA 
GATTTTAAGG ATCACATTCC AAGGGAAACT GATATGAAGG TTGCAATGAA TGTGTATGAG 
TTATCATCAG CTGCCGGATT ACCTTGTGAG ATTGATCCTG CATTGGTCGT AGCTCTTTCT 
TCACAAAAAT CGGAAAACAT TAGTCCAGAA GAAGAGTATA AAATTGCCTG CCTTCTCATG 
GTGTTTGTGG CAGTTTCTTT GCCAACACTG GCCAGTAATG TGATGTCTCA GTACAGCCCT 
GCTATAGAAG GGCATTGCAA CAACATACAT TGCTTGGCCA AAGCCATCAA CCAGATTGCT 
GCAGCTTTGT TTACAATTCA CAAAGGAAGC ATTGAAGACC GTCTTAAAGA ATTTCTGGCG 
CTTGCATCCT CCAGTCTACT GAAAATTGGC CAGGAGACAG ATAAAACTAC AACAAGAAAT 
AGAGAATCCG TTTATTTACT GCTAGATATG ATTGTACAAG AATCTCCATT CCTTACAATG 
GATCTTTTGG AATCTTGTTT TCCTTATGTC TTGCTGAGAA ATGCATACCA TGCTGTCTAC 
AAACAAAGTG TTACATCTTC TGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP1 ( XM_003309356.4 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_003309404.1 Pan troglodytes NCK associated protein 1 (NCKAP1), transcript variant X3, mRNA.

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CloneID	OPc05680
Clone ID Related Accession (Same CDS sequence)	XM_003309356.3 , XM_003309356.4
Accession Version	XM_003309356.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	nck-associated protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036881.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_003309356.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCGCT CAGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGGC ATGTGGAGAC 
CCCAAGGCAA AACCATCCTA TCTTATCGAC AAAAACCTGG AATCTGCTGT GAAATTCATA 
GTCAGAAAAT TCCCTGCTGT AGAAACCCGC AACAACAATC AACAGCTTGC ACAACTACAG 
AAAGAAAAAT CAGAGATTCT GAAAAATCTG GCATTATATT ACTTCACATT TGTAGATGTT 
ATGGAATTTA AGGACCATGT TTGTGAATTG CTGAATACTA TTGACGTTTG CCAAGTCTTC 
TTTGATATTA CTGTAAACTT TGATTTAACA AAGAACTACT TAGATTTAAT TATAACCTAT 
ACAACACTAA TGATACTGCT GTCTCGAATT GAAGAAAGGA AGGCAATCAT TGGATTATAC 
AACTATGCCC ATGAAATGAC TCATGGAGCA AGTGACAGAG AATACCCACG CCTTGGCCAG 
ATGATTGTGG ATTATGAAAA CCCTTTAAAG AAGATGATGG AAGAATTTGT ACCCCATAGC 
AAGTCTCTTT CAGATGCACT AATTTCTCTT CAAATGGTAT ATCCTCGAAG GAATCTTTCA 
GCTGACCAGT GGAGAAATGC CCAGTTATTG AGCCTCATCA GTGCGCCTAG TACAATGCTT 
AATCCAGCGC AGTCCGACAC TATGCCTTGT GAATACCTCT CTTTGGATGC AATGGAAAAG 
TGGATTATCT TTGGCTTTAT TTTGTGCCAT GGGATCCTAA ATACTGACGC TACAGCACTG 
AACCTTTGGA AACTAGCTCT TCAAAGTAGC TCTTGCCTCT CTCTCTTTCG GGATGAAGTT 
TTCCACATTC ACAAAGCTGC AGAAGACTTA TTTGTAAACA TACGAGGCTA TAATAAACGT 
ATTAATGACA TAAGAGAATG CAAGGAGGCA GCCGTGTCAC ATGCTGGTTC AATGCACAGA 
GAAAGACGCA AGTTTTTAAG ATCTGCACTG AAGGAATTGG CTACTGTCCT CTCTGATCAA 
CCTGGATTGC TAGGTCCCAA GGCACTTTTT GTTTTTATGG CATTATCCTT TGCCCGTGAT 
GAAATCATCT GGCTACTTCG TCATGCAGAT AACATGCCAA AGAAGAGTGC AGACGACTTT 
ATAGATAAGC ACATTGCTGA ATTAATATTT TACATGGAAG AACTTAGAGC ACATGTGAGG 
AAATATGGAC CTGTAATGCA GAGGTATTAC GTGCAGTACC TTTCTGGCTT TGATGCTGTT 
GTCCTCAATG AACTCGTGCA GAATCTTTCT GTTTGCCCTG AAGATGAATC AATCATCATG 
TCCTCTTTTG TTAACACTAT GACTTCCCTA AGTGTAAAAC AAGTTGAAGA TGGGGAAGTA 
TTTGATTTCA GAGGAATGAG ATTAGATTGG TTTAGGTTAC AGGCATATAC TAGTGTCTCA 
AAGGCTTCAC TTGGCCTTGC AGATCACAGA GAACTTGGAA AGATGATGAA TACAATAATT 
TTTCATACAA AAATGGTAGA TTCCTTGGTG GAAATGTTGG TGGAAACATC AGATCTCTCC 
ATATTTTGTT TTTATAGTCG TGCTTTTGAG AAGATGTTTC AACAGTGTTT GGAGTTACCC 
TCTCAATCAA GATACTCAAT TGCATTTCCA CTACTTTGCA CTCATTTTAT GAGTTGCACA 
CATGAACTGT GTCCAGAAGA GCGACATCAT ATTGGAGATC GCAGTCTTTC CTTATGTAAT 
ATGTTCCTAG ATGAAATGGC CAAACAAGCT CGAAATCTCA TCACTGATAT TTGCACAGAA 
CAGTGTACCC TTAGTGACCA GTTGCTGCCC AAGCATTGTG CCAAAACTAT CAGTCAAGCA 
GTGAATAAGA AATCAAAAAA GCAGACTGGT AAGAAAGGGG AACCTGAAAG GGAGAAACCA 
GGTGTTGAGA GCATGAGGAA AAACAGGCTG GTTGTGACCA ACCTTGATAA ATTGCACACT 
GCACTTTCTG AGTTATGCTT CTCTATAAAT TATGTACCAA ACATGGTGGT ATGGGAACAT 
ACCTTTACCC CACGAGAATA TTTGACTTCT CATCTGGAAA TACGCTTTAC CAAGTCAATT 
GTTGGGATGA CTATGTATAA TCAAGCCACA CAGGAAATTG CAAAACCTTC AGAACTTCTA 
ACAAGTGTAA GAGCATACAT GACCGTACTC CAGTCAATAG AAAACTATGT GCAGATTGAT 
ATTACAAGAG TATTTAATAA TGTGCTTCTT CAACAAACAC AACATTTAGA CAGTCATGGA 
GAGCCAACCA TTACAAGTCT ATACACAAAT TGGTATTTGG AAACTTTGTT ACGACAAGTC 
AGCAATGGCC ATATAGCATA TTTTCCTGCA ATGAAAGCAT TTGTGAACTT ACCTACAGAA 
AATGAATTAA CATTCAATGC AGAGGAATAT TCTGACATAT CAGAAATGAG GTCATTATCA 
GAACTACTAG GCCCATATGG TATGAAGTTT CTAAGTGAAA GCCTTATGTG GCATATTTCG 
TCACAAGTTG CTGAACTTAA GAAACTTGTG GTGGAGAATG TTGATGTGTT AACACAAATG 
AGGACCAGCT TTGACAAACC AGACCAGATG GCTGCACTCT TTAAAAGATT ATCATCTGTT 
GACAGTGTCT TGAAGAGGAT GACAATAATT GGTGTAATTT TATCCTTCCG ATCATTGGCA 
CAAGAAGCAC TTAGAGATGT CTTATCCTAC CACATTCCTT TTCTTGTAAG TTCAATTGAA 
GATTTTAAGG ATCACATTCC AAGGGAAACT GATATGAAGG TTGCAATGAA TGTGTATGAG 
TTATCATCAG CTGCCGGATT ACCTTGTGAG ATTGATCCTG CATTGGTCGT AGCTCTTTCT 
TCACAAAAAT CGGAAAACAT TAGTCCAGAA GAAGAGTATA AAATTGCCTG CCTTCTCATG 
GTGTTTGTGG CAGTTTCTTT GCCAACACTG GCCAGTAATG TGATGTCTCA GTACAGCCCT 
GCTATAGAAG GGCATTGCAA CAACATACAT TGCTTGGCCA AAGCCATCAA CCAGATTGCT 
GCAGCTTTGT TTACAATTCA CAAAGGAAGC ATTGAAGACC GTCTTAAAGA ATTTCTGGCG 
CTTGCATCCT CCAGTCTACT GAAAATTGGC CAGGAGACAG ATAAAACTAC AACAAGAAAT 
AGAGAATCCG TTTATTTACT GCTAGATATG ATTGTACAAG AATCTCCATT CCTTACAATG 
GATCTTTTGG AATCTTGTTT TCCTTATGTC TTGCTGAGAA ATGCATACCA TGCTGTCTAC 
AAACAAAGTG TTACATCTTC TGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003309404.1
CDS	757..4143
Translation	MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNYLDLIITYTTLMILLSRIEERKAIIGLYNYAHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNTDATALNLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSHAGSMHRERRKFLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLGLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYVPNMVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA

Target ORF information:

RefSeq Version	XM_003309356.4
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes NCK associated protein 1 (NCKAP1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003309356.4

ORF Insert Sequence:

ATGTCGCGCT CAGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGGC ATGTGGAGAC 
CCCAAGGCAA AACCATCCTA TCTTATCGAC AAAAACCTGG AATCTGCTGT GAAATTCATA 
GTCAGAAAAT TCCCTGCTGT AGAAACCCGC AACAACAATC AACAGCTTGC ACAACTACAG 
AAAGAAAAAT CAGAGATTCT GAAAAATCTG GCATTATATT ACTTCACATT TGTAGATGTT 
ATGGAATTTA AGGACCATGT TTGTGAATTG CTGAATACTA TTGACGTTTG CCAAGTCTTC 
TTTGATATTA CTGTAAACTT TGATTTAACA AAGAACTACT TAGATTTAAT TATAACCTAT 
ACAACACTAA TGATACTGCT GTCTCGAATT GAAGAAAGGA AGGCAATCAT TGGATTATAC 
AACTATGCCC ATGAAATGAC TCATGGAGCA AGTGACAGAG AATACCCACG CCTTGGCCAG 
ATGATTGTGG ATTATGAAAA CCCTTTAAAG AAGATGATGG AAGAATTTGT ACCCCATAGC 
AAGTCTCTTT CAGATGCACT AATTTCTCTT CAAATGGTAT ATCCTCGAAG GAATCTTTCA 
GCTGACCAGT GGAGAAATGC CCAGTTATTG AGCCTCATCA GTGCGCCTAG TACAATGCTT 
AATCCAGCGC AGTCCGACAC TATGCCTTGT GAATACCTCT CTTTGGATGC AATGGAAAAG 
TGGATTATCT TTGGCTTTAT TTTGTGCCAT GGGATCCTAA ATACTGACGC TACAGCACTG 
AACCTTTGGA AACTAGCTCT TCAAAGTAGC TCTTGCCTCT CTCTCTTTCG GGATGAAGTT 
TTCCACATTC ACAAAGCTGC AGAAGACTTA TTTGTAAACA TACGAGGCTA TAATAAACGT 
ATTAATGACA TAAGAGAATG CAAGGAGGCA GCCGTGTCAC ATGCTGGTTC AATGCACAGA 
GAAAGACGCA AGTTTTTAAG ATCTGCACTG AAGGAATTGG CTACTGTCCT CTCTGATCAA 
CCTGGATTGC TAGGTCCCAA GGCACTTTTT GTTTTTATGG CATTATCCTT TGCCCGTGAT 
GAAATCATCT GGCTACTTCG TCATGCAGAT AACATGCCAA AGAAGAGTGC AGACGACTTT 
ATAGATAAGC ACATTGCTGA ATTAATATTT TACATGGAAG AACTTAGAGC ACATGTGAGG 
AAATATGGAC CTGTAATGCA GAGGTATTAC GTGCAGTACC TTTCTGGCTT TGATGCTGTT 
GTCCTCAATG AACTCGTGCA GAATCTTTCT GTTTGCCCTG AAGATGAATC AATCATCATG 
TCCTCTTTTG TTAACACTAT GACTTCCCTA AGTGTAAAAC AAGTTGAAGA TGGGGAAGTA 
TTTGATTTCA GAGGAATGAG ATTAGATTGG TTTAGGTTAC AGGCATATAC TAGTGTCTCA 
AAGGCTTCAC TTGGCCTTGC AGATCACAGA GAACTTGGAA AGATGATGAA TACAATAATT 
TTTCATACAA AAATGGTAGA TTCCTTGGTG GAAATGTTGG TGGAAACATC AGATCTCTCC 
ATATTTTGTT TTTATAGTCG TGCTTTTGAG AAGATGTTTC AACAGTGTTT GGAGTTACCC 
TCTCAATCAA GATACTCAAT TGCATTTCCA CTACTTTGCA CTCATTTTAT GAGTTGCACA 
CATGAACTGT GTCCAGAAGA GCGACATCAT ATTGGAGATC GCAGTCTTTC CTTATGTAAT 
ATGTTCCTAG ATGAAATGGC CAAACAAGCT CGAAATCTCA TCACTGATAT TTGCACAGAA 
CAGTGTACCC TTAGTGACCA GTTGCTGCCC AAGCATTGTG CCAAAACTAT CAGTCAAGCA 
GTGAATAAGA AATCAAAAAA GCAGACTGGT AAGAAAGGGG AACCTGAAAG GGAGAAACCA 
GGTGTTGAGA GCATGAGGAA AAACAGGCTG GTTGTGACCA ACCTTGATAA ATTGCACACT 
GCACTTTCTG AGTTATGCTT CTCTATAAAT TATGTACCAA ACATGGTGGT ATGGGAACAT 
ACCTTTACCC CACGAGAATA TTTGACTTCT CATCTGGAAA TACGCTTTAC CAAGTCAATT 
GTTGGGATGA CTATGTATAA TCAAGCCACA CAGGAAATTG CAAAACCTTC AGAACTTCTA 
ACAAGTGTAA GAGCATACAT GACCGTACTC CAGTCAATAG AAAACTATGT GCAGATTGAT 
ATTACAAGAG TATTTAATAA TGTGCTTCTT CAACAAACAC AACATTTAGA CAGTCATGGA 
GAGCCAACCA TTACAAGTCT ATACACAAAT TGGTATTTGG AAACTTTGTT ACGACAAGTC 
AGCAATGGCC ATATAGCATA TTTTCCTGCA ATGAAAGCAT TTGTGAACTT ACCTACAGAA 
AATGAATTAA CATTCAATGC AGAGGAATAT TCTGACATAT CAGAAATGAG GTCATTATCA 
GAACTACTAG GCCCATATGG TATGAAGTTT CTAAGTGAAA GCCTTATGTG GCATATTTCG 
TCACAAGTTG CTGAACTTAA GAAACTTGTG GTGGAGAATG TTGATGTGTT AACACAAATG 
AGGACCAGCT TTGACAAACC AGACCAGATG GCTGCACTCT TTAAAAGATT ATCATCTGTT 
GACAGTGTCT TGAAGAGGAT GACAATAATT GGTGTAATTT TATCCTTCCG ATCATTGGCA 
CAAGAAGCAC TTAGAGATGT CTTATCCTAC CACATTCCTT TTCTTGTAAG TTCAATTGAA 
GATTTTAAGG ATCACATTCC AAGGGAAACT GATATGAAGG TTGCAATGAA TGTGTATGAG 
TTATCATCAG CTGCCGGATT ACCTTGTGAG ATTGATCCTG CATTGGTCGT AGCTCTTTCT 
TCACAAAAAT CGGAAAACAT TAGTCCAGAA GAAGAGTATA AAATTGCCTG CCTTCTCATG 
GTGTTTGTGG CAGTTTCTTT GCCAACACTG GCCAGTAATG TGATGTCTCA GTACAGCCCT 
GCTATAGAAG GGCATTGCAA CAACATACAT TGCTTGGCCA AAGCCATCAA CCAGATTGCT 
GCAGCTTTGT TTACAATTCA CAAAGGAAGC ATTGAAGACC GTCTTAAAGA ATTTCTGGCG 
CTTGCATCCT CCAGTCTACT GAAAATTGGC CAGGAGACAG ATAAAACTAC AACAAGAAAT 
AGAGAATCCG TTTATTTACT GCTAGATATG ATTGTACAAG AATCTCCATT CCTTACAATG 
GATCTTTTGG AATCTTGTTT TCCTTATGTC TTGCTGAGAA ATGCATACCA TGCTGTCTAC 
AAACAAAGTG TTACATCTTC TGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP1 ( XM_001148485.5 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_001148485.2 Pan troglodytes NCK associated protein 1 (NCKAP1), transcript variant X1, mRNA.

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CloneID	OPc05681
Clone ID Related Accession (Same CDS sequence)	XM_001148485.5 , XM_001148485.6
Accession Version	XM_001148485.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	nck-associated protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006470.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_001148485.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCGCT CAGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGCA AGGACAAGTT 
TGGAAGGCAT GTGGAGACCC CAAGGCAAAA CCATCCTATC TTATCGACAA AAACCTGGAA 
TCTGCTGTGA AATTCATAGT CAGAAAATTC CCTGCTGTAG AAACCCGCAA CAACAATCAA 
CAGCTTGCAC AACTACAGAA AGAAAAATCA GAGATTCTGA AAAATCTGGC ATTATATTAC 
TTCACATTTG TAGATGTTAT GGAATTTAAG GACCATGTTT GTGAATTGCT GAATACTATT 
GACGTTTGCC AAGTCTTCTT TGATATTACT GTAAACTTTG ATTTAACAAA GAACTACTTA 
GATTTAATTA TAACCTATAC AACACTAATG ATACTGCTGT CTCGAATTGA AGAAAGGAAG 
GCAATCATTG GATTATACAA CTATGCCCAT GAAATGACTC ATGGAGCAAG TGACAGAGAA 
TACCCACGCC TTGGCCAGAT GATTGTGGAT TATGAAAACC CTTTAAAGAA GATGATGGAA 
GAATTTGTAC CCCATAGCAA GTCTCTTTCA GATGCACTAA TTTCTCTTCA AATGGTATAT 
CCTCGAAGGA ATCTTTCAGC TGACCAGTGG AGAAATGCCC AGTTATTGAG CCTCATCAGT 
GCGCCTAGTA CAATGCTTAA TCCAGCGCAG TCCGACACTA TGCCTTGTGA ATACCTCTCT 
TTGGATGCAA TGGAAAAGTG GATTATCTTT GGCTTTATTT TGTGCCATGG GATCCTAAAT 
ACTGACGCTA CAGCACTGAA CCTTTGGAAA CTAGCTCTTC AAAGTAGCTC TTGCCTCTCT 
CTCTTTCGGG ATGAAGTTTT CCACATTCAC AAAGCTGCAG AAGACTTATT TGTAAACATA 
CGAGGCTATA ATAAACGTAT TAATGACATA AGAGAATGCA AGGAGGCAGC CGTGTCACAT 
GCTGGTTCAA TGCACAGAGA AAGACGCAAG TTTTTAAGAT CTGCACTGAA GGAATTGGCT 
ACTGTCCTCT CTGATCAACC TGGATTGCTA GGTCCCAAGG CACTTTTTGT TTTTATGGCA 
TTATCCTTTG CCCGTGATGA AATCATCTGG CTACTTCGTC ATGCAGATAA CATGCCAAAG 
AAGAGTGCAG ACGACTTTAT AGATAAGCAC ATTGCTGAAT TAATATTTTA CATGGAAGAA 
CTTAGAGCAC ATGTGAGGAA ATATGGACCT GTAATGCAGA GGTATTACGT GCAGTACCTT 
TCTGGCTTTG ATGCTGTTGT CCTCAATGAA CTCGTGCAGA ATCTTTCTGT TTGCCCTGAA 
GATGAATCAA TCATCATGTC CTCTTTTGTT AACACTATGA CTTCCCTAAG TGTAAAACAA 
GTTGAAGATG GGGAAGTATT TGATTTCAGA GGAATGAGAT TAGATTGGTT TAGGTTACAG 
GCATATACTA GTGTCTCAAA GGCTTCACTT GGCCTTGCAG ATCACAGAGA ACTTGGAAAG 
ATGATGAATA CAATAATTTT TCATACAAAA ATGGTAGATT CCTTGGTGGA AATGTTGGTG 
GAAACATCAG ATCTCTCCAT ATTTTGTTTT TATAGTCGTG CTTTTGAGAA GATGTTTCAA 
CAGTGTTTGG AGTTACCCTC TCAATCAAGA TACTCAATTG CATTTCCACT ACTTTGCACT 
CATTTTATGA GTTGCACACA TGAACTGTGT CCAGAAGAGC GACATCATAT TGGAGATCGC 
AGTCTTTCCT TATGTAATAT GTTCCTAGAT GAAATGGCCA AACAAGCTCG AAATCTCATC 
ACTGATATTT GCACAGAACA GTGTACCCTT AGTGACCAGT TGCTGCCCAA GCATTGTGCC 
AAAACTATCA GTCAAGCAGT GAATAAGAAA TCAAAAAAGC AGACTGGTAA GAAAGGGGAA 
CCTGAAAGGG AGAAACCAGG TGTTGAGAGC ATGAGGAAAA ACAGGCTGGT TGTGACCAAC 
CTTGATAAAT TGCACACTGC ACTTTCTGAG TTATGCTTCT CTATAAATTA TGTACCAAAC 
ATGGTGGTAT GGGAACATAC CTTTACCCCA CGAGAATATT TGACTTCTCA TCTGGAAATA 
CGCTTTACCA AGTCAATTGT TGGGATGACT ATGTATAATC AAGCCACACA GGAAATTGCA 
AAACCTTCAG AACTTCTAAC AAGTGTAAGA GCATACATGA CCGTACTCCA GTCAATAGAA 
AACTATGTGC AGATTGATAT TACAAGAGTA TTTAATAATG TGCTTCTTCA ACAAACACAA 
CATTTAGACA GTCATGGAGA GCCAACCATT ACAAGTCTAT ACACAAATTG GTATTTGGAA 
ACTTTGTTAC GACAAGTCAG CAATGGCCAT ATAGCATATT TTCCTGCAAT GAAAGCATTT 
GTGAACTTAC CTACAGAAAA TGAATTAACA TTCAATGCAG AGGAATATTC TGACATATCA 
GAAATGAGGT CATTATCAGA ACTACTAGGC CCATATGGTA TGAAGTTTCT AAGTGAAAGC 
CTTATGTGGC ATATTTCGTC ACAAGTTGCT GAACTTAAGA AACTTGTGGT GGAGAATGTT 
GATGTGTTAA CACAAATGAG GACCAGCTTT GACAAACCAG ACCAGATGGC TGCACTCTTT 
AAAAGATTAT CATCTGTTGA CAGTGTCTTG AAGAGGATGA CAATAATTGG TGTAATTTTA 
TCCTTCCGAT CATTGGCACA AGAAGCACTT AGAGATGTCT TATCCTACCA CATTCCTTTT 
CTTGTAAGTT CAATTGAAGA TTTTAAGGAT CACATTCCAA GGGAAACTGA TATGAAGGTT 
GCAATGAATG TGTATGAGTT ATCATCAGCT GCCGGATTAC CTTGTGAGAT TGATCCTGCA 
TTGGTCGTAG CTCTTTCTTC ACAAAAATCG GAAAACATTA GTCCAGAAGA AGAGTATAAA 
ATTGCCTGCC TTCTCATGGT GTTTGTGGCA GTTTCTTTGC CAACACTGGC CAGTAATGTG 
ATGTCTCAGT ACAGCCCTGC TATAGAAGGG CATTGCAACA ACATACATTG CTTGGCCAAA 
GCCATCAACC AGATTGCTGC AGCTTTGTTT ACAATTCACA AAGGAAGCAT TGAAGACCGT 
CTTAAAGAAT TTCTGGCGCT TGCATCCTCC AGTCTACTGA AAATTGGCCA GGAGACAGAT 
AAAACTACAA CAAGAAATAG AGAATCCGTT TATTTACTGC TAGATATGAT TGTACAAGAA 
TCTCCATTCC TTACAATGGA TCTTTTGGAA TCTTGTTTTC CTTATGTCTT GCTGAGAAAT 
GCATACCATG CTGTCTACAA ACAAAGTGTT ACATCTTCTG CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001148485.2
CDS	988..4392
Translation	MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKQGQVWKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNYLDLIITYTTLMILLSRIEERKAIIGLYNYAHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNTDATALNLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSHAGSMHRERRKFLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLGLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYVPNMVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA

Target ORF information:

RefSeq Version	XM_001148485.5
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes NCK associated protein 1 (NCKAP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001148485.5

ORF Insert Sequence:

ATGTCGCGCT CAGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGCA AGGACAAGTT 
TGGAAGGCAT GTGGAGACCC CAAGGCAAAA CCATCCTATC TTATCGACAA AAACCTGGAA 
TCTGCTGTGA AATTCATAGT CAGAAAATTC CCTGCTGTAG AAACCCGCAA CAACAATCAA 
CAGCTTGCAC AACTACAGAA AGAAAAATCA GAGATTCTGA AAAATCTGGC ATTATATTAC 
TTCACATTTG TAGATGTTAT GGAATTTAAG GACCATGTTT GTGAATTGCT GAATACTATT 
GACGTTTGCC AAGTCTTCTT TGATATTACT GTAAACTTTG ATTTAACAAA GAACTACTTA 
GATTTAATTA TAACCTATAC AACACTAATG ATACTGCTGT CTCGAATTGA AGAAAGGAAG 
GCAATCATTG GATTATACAA CTATGCCCAT GAAATGACTC ATGGAGCAAG TGACAGAGAA 
TACCCACGCC TTGGCCAGAT GATTGTGGAT TATGAAAACC CTTTAAAGAA GATGATGGAA 
GAATTTGTAC CCCATAGCAA GTCTCTTTCA GATGCACTAA TTTCTCTTCA AATGGTATAT 
CCTCGAAGGA ATCTTTCAGC TGACCAGTGG AGAAATGCCC AGTTATTGAG CCTCATCAGT 
GCGCCTAGTA CAATGCTTAA TCCAGCGCAG TCCGACACTA TGCCTTGTGA ATACCTCTCT 
TTGGATGCAA TGGAAAAGTG GATTATCTTT GGCTTTATTT TGTGCCATGG GATCCTAAAT 
ACTGACGCTA CAGCACTGAA CCTTTGGAAA CTAGCTCTTC AAAGTAGCTC TTGCCTCTCT 
CTCTTTCGGG ATGAAGTTTT CCACATTCAC AAAGCTGCAG AAGACTTATT TGTAAACATA 
CGAGGCTATA ATAAACGTAT TAATGACATA AGAGAATGCA AGGAGGCAGC CGTGTCACAT 
GCTGGTTCAA TGCACAGAGA AAGACGCAAG TTTTTAAGAT CTGCACTGAA GGAATTGGCT 
ACTGTCCTCT CTGATCAACC TGGATTGCTA GGTCCCAAGG CACTTTTTGT TTTTATGGCA 
TTATCCTTTG CCCGTGATGA AATCATCTGG CTACTTCGTC ATGCAGATAA CATGCCAAAG 
AAGAGTGCAG ACGACTTTAT AGATAAGCAC ATTGCTGAAT TAATATTTTA CATGGAAGAA 
CTTAGAGCAC ATGTGAGGAA ATATGGACCT GTAATGCAGA GGTATTACGT GCAGTACCTT 
TCTGGCTTTG ATGCTGTTGT CCTCAATGAA CTCGTGCAGA ATCTTTCTGT TTGCCCTGAA 
GATGAATCAA TCATCATGTC CTCTTTTGTT AACACTATGA CTTCCCTAAG TGTAAAACAA 
GTTGAAGATG GGGAAGTATT TGATTTCAGA GGAATGAGAT TAGATTGGTT TAGGTTACAG 
GCATATACTA GTGTCTCAAA GGCTTCACTT GGCCTTGCAG ATCACAGAGA ACTTGGAAAG 
ATGATGAATA CAATAATTTT TCATACAAAA ATGGTAGATT CCTTGGTGGA AATGTTGGTG 
GAAACATCAG ATCTCTCCAT ATTTTGTTTT TATAGTCGTG CTTTTGAGAA GATGTTTCAA 
CAGTGTTTGG AGTTACCCTC TCAATCAAGA TACTCAATTG CATTTCCACT ACTTTGCACT 
CATTTTATGA GTTGCACACA TGAACTGTGT CCAGAAGAGC GACATCATAT TGGAGATCGC 
AGTCTTTCCT TATGTAATAT GTTCCTAGAT GAAATGGCCA AACAAGCTCG AAATCTCATC 
ACTGATATTT GCACAGAACA GTGTACCCTT AGTGACCAGT TGCTGCCCAA GCATTGTGCC 
AAAACTATCA GTCAAGCAGT GAATAAGAAA TCAAAAAAGC AGACTGGTAA GAAAGGGGAA 
CCTGAAAGGG AGAAACCAGG TGTTGAGAGC ATGAGGAAAA ACAGGCTGGT TGTGACCAAC 
CTTGATAAAT TGCACACTGC ACTTTCTGAG TTATGCTTCT CTATAAATTA TGTACCAAAC 
ATGGTGGTAT GGGAACATAC CTTTACCCCA CGAGAATATT TGACTTCTCA TCTGGAAATA 
CGCTTTACCA AGTCAATTGT TGGGATGACT ATGTATAATC AAGCCACACA GGAAATTGCA 
AAACCTTCAG AACTTCTAAC AAGTGTAAGA GCATACATGA CCGTACTCCA GTCAATAGAA 
AACTATGTGC AGATTGATAT TACAAGAGTA TTTAATAATG TGCTTCTTCA ACAAACACAA 
CATTTAGACA GTCATGGAGA GCCAACCATT ACAAGTCTAT ACACAAATTG GTATTTGGAA 
ACTTTGTTAC GACAAGTCAG CAATGGCCAT ATAGCATATT TTCCTGCAAT GAAAGCATTT 
GTGAACTTAC CTACAGAAAA TGAATTAACA TTCAATGCAG AGGAATATTC TGACATATCA 
GAAATGAGGT CATTATCAGA ACTACTAGGC CCATATGGTA TGAAGTTTCT AAGTGAAAGC 
CTTATGTGGC ATATTTCGTC ACAAGTTGCT GAACTTAAGA AACTTGTGGT GGAGAATGTT 
GATGTGTTAA CACAAATGAG GACCAGCTTT GACAAACCAG ACCAGATGGC TGCACTCTTT 
AAAAGATTAT CATCTGTTGA CAGTGTCTTG AAGAGGATGA CAATAATTGG TGTAATTTTA 
TCCTTCCGAT CATTGGCACA AGAAGCACTT AGAGATGTCT TATCCTACCA CATTCCTTTT 
CTTGTAAGTT CAATTGAAGA TTTTAAGGAT CACATTCCAA GGGAAACTGA TATGAAGGTT 
GCAATGAATG TGTATGAGTT ATCATCAGCT GCCGGATTAC CTTGTGAGAT TGATCCTGCA 
TTGGTCGTAG CTCTTTCTTC ACAAAAATCG GAAAACATTA GTCCAGAAGA AGAGTATAAA 
ATTGCCTGCC TTCTCATGGT GTTTGTGGCA GTTTCTTTGC CAACACTGGC CAGTAATGTG 
ATGTCTCAGT ACAGCCCTGC TATAGAAGGG CATTGCAACA ACATACATTG CTTGGCCAAA 
GCCATCAACC AGATTGCTGC AGCTTTGTTT ACAATTCACA AAGGAAGCAT TGAAGACCGT 
CTTAAAGAAT TTCTGGCGCT TGCATCCTCC AGTCTACTGA AAATTGGCCA GGAGACAGAT 
AAAACTACAA CAAGAAATAG AGAATCCGTT TATTTACTGC TAGATATGAT TGTACAAGAA 
TCTCCATTCC TTACAATGGA TCTTTTGGAA TCTTGTTTTC CTTATGTCTT GCTGAGAAAT 
GCATACCATG CTGTCTACAA ACAAAGTGTT ACATCTTCTG CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP1 ( XM_001148485.6 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_001148485.2 Pan troglodytes NCK associated protein 1 (NCKAP1), transcript variant X1, mRNA.

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CloneID	OPc05681
Clone ID Related Accession (Same CDS sequence)	XM_001148485.5 , XM_001148485.6
Accession Version	XM_001148485.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	nck-associated protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036881.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_001148485.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCGCT CAGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGCA AGGACAAGTT 
TGGAAGGCAT GTGGAGACCC CAAGGCAAAA CCATCCTATC TTATCGACAA AAACCTGGAA 
TCTGCTGTGA AATTCATAGT CAGAAAATTC CCTGCTGTAG AAACCCGCAA CAACAATCAA 
CAGCTTGCAC AACTACAGAA AGAAAAATCA GAGATTCTGA AAAATCTGGC ATTATATTAC 
TTCACATTTG TAGATGTTAT GGAATTTAAG GACCATGTTT GTGAATTGCT GAATACTATT 
GACGTTTGCC AAGTCTTCTT TGATATTACT GTAAACTTTG ATTTAACAAA GAACTACTTA 
GATTTAATTA TAACCTATAC AACACTAATG ATACTGCTGT CTCGAATTGA AGAAAGGAAG 
GCAATCATTG GATTATACAA CTATGCCCAT GAAATGACTC ATGGAGCAAG TGACAGAGAA 
TACCCACGCC TTGGCCAGAT GATTGTGGAT TATGAAAACC CTTTAAAGAA GATGATGGAA 
GAATTTGTAC CCCATAGCAA GTCTCTTTCA GATGCACTAA TTTCTCTTCA AATGGTATAT 
CCTCGAAGGA ATCTTTCAGC TGACCAGTGG AGAAATGCCC AGTTATTGAG CCTCATCAGT 
GCGCCTAGTA CAATGCTTAA TCCAGCGCAG TCCGACACTA TGCCTTGTGA ATACCTCTCT 
TTGGATGCAA TGGAAAAGTG GATTATCTTT GGCTTTATTT TGTGCCATGG GATCCTAAAT 
ACTGACGCTA CAGCACTGAA CCTTTGGAAA CTAGCTCTTC AAAGTAGCTC TTGCCTCTCT 
CTCTTTCGGG ATGAAGTTTT CCACATTCAC AAAGCTGCAG AAGACTTATT TGTAAACATA 
CGAGGCTATA ATAAACGTAT TAATGACATA AGAGAATGCA AGGAGGCAGC CGTGTCACAT 
GCTGGTTCAA TGCACAGAGA AAGACGCAAG TTTTTAAGAT CTGCACTGAA GGAATTGGCT 
ACTGTCCTCT CTGATCAACC TGGATTGCTA GGTCCCAAGG CACTTTTTGT TTTTATGGCA 
TTATCCTTTG CCCGTGATGA AATCATCTGG CTACTTCGTC ATGCAGATAA CATGCCAAAG 
AAGAGTGCAG ACGACTTTAT AGATAAGCAC ATTGCTGAAT TAATATTTTA CATGGAAGAA 
CTTAGAGCAC ATGTGAGGAA ATATGGACCT GTAATGCAGA GGTATTACGT GCAGTACCTT 
TCTGGCTTTG ATGCTGTTGT CCTCAATGAA CTCGTGCAGA ATCTTTCTGT TTGCCCTGAA 
GATGAATCAA TCATCATGTC CTCTTTTGTT AACACTATGA CTTCCCTAAG TGTAAAACAA 
GTTGAAGATG GGGAAGTATT TGATTTCAGA GGAATGAGAT TAGATTGGTT TAGGTTACAG 
GCATATACTA GTGTCTCAAA GGCTTCACTT GGCCTTGCAG ATCACAGAGA ACTTGGAAAG 
ATGATGAATA CAATAATTTT TCATACAAAA ATGGTAGATT CCTTGGTGGA AATGTTGGTG 
GAAACATCAG ATCTCTCCAT ATTTTGTTTT TATAGTCGTG CTTTTGAGAA GATGTTTCAA 
CAGTGTTTGG AGTTACCCTC TCAATCAAGA TACTCAATTG CATTTCCACT ACTTTGCACT 
CATTTTATGA GTTGCACACA TGAACTGTGT CCAGAAGAGC GACATCATAT TGGAGATCGC 
AGTCTTTCCT TATGTAATAT GTTCCTAGAT GAAATGGCCA AACAAGCTCG AAATCTCATC 
ACTGATATTT GCACAGAACA GTGTACCCTT AGTGACCAGT TGCTGCCCAA GCATTGTGCC 
AAAACTATCA GTCAAGCAGT GAATAAGAAA TCAAAAAAGC AGACTGGTAA GAAAGGGGAA 
CCTGAAAGGG AGAAACCAGG TGTTGAGAGC ATGAGGAAAA ACAGGCTGGT TGTGACCAAC 
CTTGATAAAT TGCACACTGC ACTTTCTGAG TTATGCTTCT CTATAAATTA TGTACCAAAC 
ATGGTGGTAT GGGAACATAC CTTTACCCCA CGAGAATATT TGACTTCTCA TCTGGAAATA 
CGCTTTACCA AGTCAATTGT TGGGATGACT ATGTATAATC AAGCCACACA GGAAATTGCA 
AAACCTTCAG AACTTCTAAC AAGTGTAAGA GCATACATGA CCGTACTCCA GTCAATAGAA 
AACTATGTGC AGATTGATAT TACAAGAGTA TTTAATAATG TGCTTCTTCA ACAAACACAA 
CATTTAGACA GTCATGGAGA GCCAACCATT ACAAGTCTAT ACACAAATTG GTATTTGGAA 
ACTTTGTTAC GACAAGTCAG CAATGGCCAT ATAGCATATT TTCCTGCAAT GAAAGCATTT 
GTGAACTTAC CTACAGAAAA TGAATTAACA TTCAATGCAG AGGAATATTC TGACATATCA 
GAAATGAGGT CATTATCAGA ACTACTAGGC CCATATGGTA TGAAGTTTCT AAGTGAAAGC 
CTTATGTGGC ATATTTCGTC ACAAGTTGCT GAACTTAAGA AACTTGTGGT GGAGAATGTT 
GATGTGTTAA CACAAATGAG GACCAGCTTT GACAAACCAG ACCAGATGGC TGCACTCTTT 
AAAAGATTAT CATCTGTTGA CAGTGTCTTG AAGAGGATGA CAATAATTGG TGTAATTTTA 
TCCTTCCGAT CATTGGCACA AGAAGCACTT AGAGATGTCT TATCCTACCA CATTCCTTTT 
CTTGTAAGTT CAATTGAAGA TTTTAAGGAT CACATTCCAA GGGAAACTGA TATGAAGGTT 
GCAATGAATG TGTATGAGTT ATCATCAGCT GCCGGATTAC CTTGTGAGAT TGATCCTGCA 
TTGGTCGTAG CTCTTTCTTC ACAAAAATCG GAAAACATTA GTCCAGAAGA AGAGTATAAA 
ATTGCCTGCC TTCTCATGGT GTTTGTGGCA GTTTCTTTGC CAACACTGGC CAGTAATGTG 
ATGTCTCAGT ACAGCCCTGC TATAGAAGGG CATTGCAACA ACATACATTG CTTGGCCAAA 
GCCATCAACC AGATTGCTGC AGCTTTGTTT ACAATTCACA AAGGAAGCAT TGAAGACCGT 
CTTAAAGAAT TTCTGGCGCT TGCATCCTCC AGTCTACTGA AAATTGGCCA GGAGACAGAT 
AAAACTACAA CAAGAAATAG AGAATCCGTT TATTTACTGC TAGATATGAT TGTACAAGAA 
TCTCCATTCC TTACAATGGA TCTTTTGGAA TCTTGTTTTC CTTATGTCTT GCTGAGAAAT 
GCATACCATG CTGTCTACAA ACAAAGTGTT ACATCTTCTG CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001148485.2
CDS	757..4161
Translation	MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKQGQVWKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNYLDLIITYTTLMILLSRIEERKAIIGLYNYAHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNTDATALNLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSHAGSMHRERRKFLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLGLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYVPNMVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA

Target ORF information:

RefSeq Version	XM_001148485.6
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes NCK associated protein 1 (NCKAP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001148485.6

ORF Insert Sequence:

ATGTCGCGCT CAGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGCA AGGACAAGTT 
TGGAAGGCAT GTGGAGACCC CAAGGCAAAA CCATCCTATC TTATCGACAA AAACCTGGAA 
TCTGCTGTGA AATTCATAGT CAGAAAATTC CCTGCTGTAG AAACCCGCAA CAACAATCAA 
CAGCTTGCAC AACTACAGAA AGAAAAATCA GAGATTCTGA AAAATCTGGC ATTATATTAC 
TTCACATTTG TAGATGTTAT GGAATTTAAG GACCATGTTT GTGAATTGCT GAATACTATT 
GACGTTTGCC AAGTCTTCTT TGATATTACT GTAAACTTTG ATTTAACAAA GAACTACTTA 
GATTTAATTA TAACCTATAC AACACTAATG ATACTGCTGT CTCGAATTGA AGAAAGGAAG 
GCAATCATTG GATTATACAA CTATGCCCAT GAAATGACTC ATGGAGCAAG TGACAGAGAA 
TACCCACGCC TTGGCCAGAT GATTGTGGAT TATGAAAACC CTTTAAAGAA GATGATGGAA 
GAATTTGTAC CCCATAGCAA GTCTCTTTCA GATGCACTAA TTTCTCTTCA AATGGTATAT 
CCTCGAAGGA ATCTTTCAGC TGACCAGTGG AGAAATGCCC AGTTATTGAG CCTCATCAGT 
GCGCCTAGTA CAATGCTTAA TCCAGCGCAG TCCGACACTA TGCCTTGTGA ATACCTCTCT 
TTGGATGCAA TGGAAAAGTG GATTATCTTT GGCTTTATTT TGTGCCATGG GATCCTAAAT 
ACTGACGCTA CAGCACTGAA CCTTTGGAAA CTAGCTCTTC AAAGTAGCTC TTGCCTCTCT 
CTCTTTCGGG ATGAAGTTTT CCACATTCAC AAAGCTGCAG AAGACTTATT TGTAAACATA 
CGAGGCTATA ATAAACGTAT TAATGACATA AGAGAATGCA AGGAGGCAGC CGTGTCACAT 
GCTGGTTCAA TGCACAGAGA AAGACGCAAG TTTTTAAGAT CTGCACTGAA GGAATTGGCT 
ACTGTCCTCT CTGATCAACC TGGATTGCTA GGTCCCAAGG CACTTTTTGT TTTTATGGCA 
TTATCCTTTG CCCGTGATGA AATCATCTGG CTACTTCGTC ATGCAGATAA CATGCCAAAG 
AAGAGTGCAG ACGACTTTAT AGATAAGCAC ATTGCTGAAT TAATATTTTA CATGGAAGAA 
CTTAGAGCAC ATGTGAGGAA ATATGGACCT GTAATGCAGA GGTATTACGT GCAGTACCTT 
TCTGGCTTTG ATGCTGTTGT CCTCAATGAA CTCGTGCAGA ATCTTTCTGT TTGCCCTGAA 
GATGAATCAA TCATCATGTC CTCTTTTGTT AACACTATGA CTTCCCTAAG TGTAAAACAA 
GTTGAAGATG GGGAAGTATT TGATTTCAGA GGAATGAGAT TAGATTGGTT TAGGTTACAG 
GCATATACTA GTGTCTCAAA GGCTTCACTT GGCCTTGCAG ATCACAGAGA ACTTGGAAAG 
ATGATGAATA CAATAATTTT TCATACAAAA ATGGTAGATT CCTTGGTGGA AATGTTGGTG 
GAAACATCAG ATCTCTCCAT ATTTTGTTTT TATAGTCGTG CTTTTGAGAA GATGTTTCAA 
CAGTGTTTGG AGTTACCCTC TCAATCAAGA TACTCAATTG CATTTCCACT ACTTTGCACT 
CATTTTATGA GTTGCACACA TGAACTGTGT CCAGAAGAGC GACATCATAT TGGAGATCGC 
AGTCTTTCCT TATGTAATAT GTTCCTAGAT GAAATGGCCA AACAAGCTCG AAATCTCATC 
ACTGATATTT GCACAGAACA GTGTACCCTT AGTGACCAGT TGCTGCCCAA GCATTGTGCC 
AAAACTATCA GTCAAGCAGT GAATAAGAAA TCAAAAAAGC AGACTGGTAA GAAAGGGGAA 
CCTGAAAGGG AGAAACCAGG TGTTGAGAGC ATGAGGAAAA ACAGGCTGGT TGTGACCAAC 
CTTGATAAAT TGCACACTGC ACTTTCTGAG TTATGCTTCT CTATAAATTA TGTACCAAAC 
ATGGTGGTAT GGGAACATAC CTTTACCCCA CGAGAATATT TGACTTCTCA TCTGGAAATA 
CGCTTTACCA AGTCAATTGT TGGGATGACT ATGTATAATC AAGCCACACA GGAAATTGCA 
AAACCTTCAG AACTTCTAAC AAGTGTAAGA GCATACATGA CCGTACTCCA GTCAATAGAA 
AACTATGTGC AGATTGATAT TACAAGAGTA TTTAATAATG TGCTTCTTCA ACAAACACAA 
CATTTAGACA GTCATGGAGA GCCAACCATT ACAAGTCTAT ACACAAATTG GTATTTGGAA 
ACTTTGTTAC GACAAGTCAG CAATGGCCAT ATAGCATATT TTCCTGCAAT GAAAGCATTT 
GTGAACTTAC CTACAGAAAA TGAATTAACA TTCAATGCAG AGGAATATTC TGACATATCA 
GAAATGAGGT CATTATCAGA ACTACTAGGC CCATATGGTA TGAAGTTTCT AAGTGAAAGC 
CTTATGTGGC ATATTTCGTC ACAAGTTGCT GAACTTAAGA AACTTGTGGT GGAGAATGTT 
GATGTGTTAA CACAAATGAG GACCAGCTTT GACAAACCAG ACCAGATGGC TGCACTCTTT 
AAAAGATTAT CATCTGTTGA CAGTGTCTTG AAGAGGATGA CAATAATTGG TGTAATTTTA 
TCCTTCCGAT CATTGGCACA AGAAGCACTT AGAGATGTCT TATCCTACCA CATTCCTTTT 
CTTGTAAGTT CAATTGAAGA TTTTAAGGAT CACATTCCAA GGGAAACTGA TATGAAGGTT 
GCAATGAATG TGTATGAGTT ATCATCAGCT GCCGGATTAC CTTGTGAGAT TGATCCTGCA 
TTGGTCGTAG CTCTTTCTTC ACAAAAATCG GAAAACATTA GTCCAGAAGA AGAGTATAAA 
ATTGCCTGCC TTCTCATGGT GTTTGTGGCA GTTTCTTTGC CAACACTGGC CAGTAATGTG 
ATGTCTCAGT ACAGCCCTGC TATAGAAGGG CATTGCAACA ACATACATTG CTTGGCCAAA 
GCCATCAACC AGATTGCTGC AGCTTTGTTT ACAATTCACA AAGGAAGCAT TGAAGACCGT 
CTTAAAGAAT TTCTGGCGCT TGCATCCTCC AGTCTACTGA AAATTGGCCA GGAGACAGAT 
AAAACTACAA CAAGAAATAG AGAATCCGTT TATTTACTGC TAGATATGAT TGTACAAGAA 
TCTCCATTCC TTACAATGGA TCTTTTGGAA TCTTGTTTTC CTTATGTCTT GCTGAGAAAT 
GCATACCATG CTGTCTACAA ACAAAGTGTT ACATCTTCTG CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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NCKAP1 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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