TOP2A cDNA ORF clone, Pan troglodytes(chimpanzee)

The following TOP2A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOP2A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OPc72522 XM_016931846.1
Latest version!
Pan troglodytes topoisomerase (DNA) II alpha (TOP2A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OPc99686 XM_016931846.2
Latest version!
Pan troglodytes DNA topoisomerase II alpha (TOP2A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OPc72521 XM_016931845.1
Latest version!
Pan troglodytes topoisomerase (DNA) II alpha (TOP2A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OPc99685 XM_016931845.2
Latest version!
Pan troglodytes DNA topoisomerase II alpha (TOP2A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OPc72522
    Clone ID Related Accession (Same CDS sequence) XM_016931846.1
    Accession Version XM_016931846.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4095bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product DNA topoisomerase 2-alpha isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006484.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAAGTGT CACCATTGCA GCCCGTAAAT GAAAATATGC AAGTCAACAA AATAAAGAAA 
    AATGAAGATG CTAAGAAAAG ACTGTCTGTT GAAAGAATCT ATCAAAAGAA AACACAATTG
    GAACATATTT TGCTCCGCCC AGACACCTAC ATTGGTTCTG TGGAATTAGT GACCCAGCAA
    ATGTGGGTTT ACGATGAAGA TGTTGGCATT AACTATAGGG AAGTCACTTT TGTTCCTGGT
    TTGTACAAAA TCTTTGATGA GATTCTAGTT AATGCTGCGG ACAACAAACA AAGGGACCCA
    AAAATGTCTT GTATTAGAGT CACAATTGAT CCGGAAAACA ATTTAATTAG TATATGGAAT
    AATGGAAAAG GTATTCCTGT TGTTGAACAC AAAGTTGAAA AGATGTATGT CCCAGCTCTC
    ATATTTGGAC AGCTCCTAAC TTCTAGTAAC TATGATGATG ATGAAAAGAA AGTGACAGGT
    GGTCGAAATG GCTATGGAGC CAAATTGTGT AACATATTCA GTACCAAATT TACTGTGGAA
    ACAGCCAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA TATGGGAAGA
    GCTGGTGAGA TGGAACTCAA GCCCTTCAAT GGAGAAGATT ATACATGTAT CACCTTTCAG
    CCTGATTTGT CTAAGTTTAA AATGCAAAGC CTGGACAAAG ATATTGTTGC ACTAATGGTC
    AGAAGAGCAT ATGATATTGC TGGATCCACC AAAGATGTCA AAGTCTTTCT TAATGGAAAT
    AAACTGCCAG TAAAAGGATT TCGTAGTTAT GTGGACATGT ATTTGAAGGA CAAGTTGGAT
    GAAACTGGTA ACTCCTTGAA AGTAATACAT GAACAAGTAA ACCACAGGTG GGAAGTGTGT
    TTAACTATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTTG TCAACAGCAT TGCTACATCC
    AAGGGTGGCA GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT
    GTGAAGAAGA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTGAA AAATCACATG
    TGGATTTTTG TAAATGCCTT AATTGAAAAC CCAACCTTTG ACTCTCAGAC AAAAGAAAAC
    ATGACTTTAC AACCCAAGAG CTTTGGATCA ACCTGCCAAT TGAGTGAAAA ATTTATCAAA
    GCTGCCATTG GCTGTGGTAT TGTAGAAAGC ATACTAAACT GGGTGAAGTT TAAGGCCCAA
    GTCCAGTTAA ACAAGAAGTG TTCAGCTGTA AAACATAATA GAATCAAGGG AATTCCCAAA
    CTCGATGATG CCAATGATGC AGGGGGCCGA AACTCCACTG AGTGTACGCT TATCCTGACT
    GAGGGAGATT CAGCCAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAA
    TATGGGGTTT TCCCTCTTAG AGGAAAAATA CTCAATGTTC GAGAAGCTTC TCATAAGCAG
    ATCATGGAAA ATGCTGAGAT TAACAATATC ATCAAGATTG TGGGTCTTCA GTACAAGAAA
    AACTATGAAG ATGAAGATTC ATTGAAGACT CTTCGTTATG GGAAGATAAT GATTATGACA
    GATCAGGACC AAGATGGTTC CCACATCAAA GGCTTGCTGA TTAATTTTAT CCATCACAAC
    TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCCAT TGTAAAGGTG
    TCTAAAAACA AGCAAGAAAT GGCATTTTAC AGCCTTCCTG AATTTGAAGA ATGGAAGAGT
    TCTACTCCAA ATCATAAAAA ATGGAAAGTC AAATATTACA AAGGTTTGGG CACCAGCACA
    TCAAAGGAAG CTAAAGAATA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA GTTCAAATAT
    TCTGGTCCTG AAGATGATGC TGCTATCAGC CTGGCCTTTA GCAAAAAACA GATAGATGAT
    CGAAAGGAAT GGTTAACTAA TTTCATGGAG GATAGAAGAC AACGAAAGTT ACTTGGGCTT
    CCTGAGGATT ACTTGTATGG ACAAACTACC ACATATCTGA CATATAATGA CTTCATCAAC
    AAGGAACTTA TCTTGTTCTC AAATTCTGAT AACGAGAGAT CTATCCCTTC TATGGTGGAT
    GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACTTGCT TCAAACGGAA TGACAAGCGA
    GAGGTAAAGG TTGCCCAATT AGCTGGATCA GTGGCTGAAA TGTCTTCTTA TCATCATGGT
    GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ATTTTGTGGG TAGCAATAAT
    CTAAACCTCT TGCAGCCCAT TGGTCAGTTT GGTACCAGGC TACATGGTGG CAAGGATTCT
    GCTAGTCCAC GATACATCTT TACAATGCTC AGCTCTTTGG CTCGATTGTT ATTTCCACCA
    AAAGATGATC ACACGTTGAA GTTTTTATAT GATGACAACC AGCGTGTTGA GCCTGAATGG
    TACATTCCTA TTATTCCCAT GGTGCTGATA AATGGTGCTG AAGGAATCGG TACTGGGTGG
    TCCTGCAAAA TCCCCAACTT TGATGTGCGT GAAATTGTAA ATAACATCAG GCGTTTGATG
    GATGGAGAAG AACCATTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACTATTGAA
    GAACTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTAG CTATTCTTAA TTCTACAACC
    ATTGAAATCT CAGAGCTTCC CATCAGAACA TGGACCCAGA CATACAAAGA ACAAGTTCTA
    GAACCCATGT TGAATGGCAC CGAGAAGACA CCTCCTCTCA TAACAGACTA TAGGGAATAC
    CATACAGATA CCACTGTGAA ATTTGTTGTG AAGATGACTG AAGAAAAACT GGCAGAGGCA
    GAGAGAGTTG GACTACACAA AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCTATG
    GTGCTTTTTG ACCACGTAGG CTGTTTAAAG AAATATGACA CGGTGTTGGA TATTCTAAGA
    GACTTTTTTG AACTCAGACT TAAATATTAT GGATTAAGAA AAGAATGGCT CCTAGGAATG
    CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT
    GGCAAAATAA TCATTGAAAA TAAGCCTAAG AAAGAATTAA TTAAAGTTCT GATTCAGAGG
    GGATATGATT CGGATCCTGT GAAGGCCTGG AAAGAAGCCC AGCAAAAGGT TCCAGATGAA
    GAAGAAAATG AAGAGAGTGA CAACGAAAAG GAAACTGAAA AGAGTGACTC CGTAACAGAT
    TCTGGACCAA CCTTCAACTA TCTTCTTGAT ATGCCCCTTT GGTATTTAAC CAAGGAAAAG
    AAAGATGAAC TCTGCAGGCT AAGAAATGAA AAAGAACAAG AGCTGGACAC ATTAAAAAGA
    AAGAGTCCAT CAGATTTGTG GAAAGAAGAC TTGGCTACAT TTATTGAAGA ATTGGAGGCT
    GTTGAAGCCA AGGAAAAACA AGATGAACAA GTCGGACTTC CTGGGAAAGG GGGGAAGGCC
    AAGGGGAAAA AAACACAAAT GGCTGAAGTT TTGCCTTCTC CACGTGGTCA AAGAGTCATT
    CCACGAATAA CCATAGAAAT GAAAGCAGAG GCAGAAAAGA AAAATAAAAA GAAAATTAAG
    AATGAAAATA CTGAAGGAAG CCCTCAAGAA GATGGTGTGG AACTAGAAGG CCTAAAACAA
    AGATTAGAAA AGAAACAGAA AAGAGAACCA GGTACAAAGA CAAAGAAACA AACTACATTG
    CCATTTAAGC CAATCAAAAA AGGAAAGAAG AGAAATCCCT GGTCTGATTC AGAATCAGAT
    AGGAGCAGTG ACGAAAGTAA TTTTGATGTC CCTCCACGAG AAACAGAGCC ACGGAGAGCA
    GCAACAAAAA CCAAATTCAC AATGGATTTG GATTCAGATG AAGATTTCTC AGATTTTGAT
    GAAAAAACTG ATGATGAAGA TTTTGTCCCA TCAGATGCTA GTCCACCTAA GACCAAAACT
    TCCCCAAAAC CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016787335.1
    CDS155..4249
    Translation

    Target ORF information:

    RefSeq Version XM_016931846.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes topoisomerase (DNA) II alpha (TOP2A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016931846.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAAGTGT CACCATTGCA GCCCGTAAAT GAAAATATGC AAGTCAACAA AATAAAGAAA 
    AATGAAGATG CTAAGAAAAG ACTGTCTGTT GAAAGAATCT ATCAAAAGAA AACACAATTG
    GAACATATTT TGCTCCGCCC AGACACCTAC ATTGGTTCTG TGGAATTAGT GACCCAGCAA
    ATGTGGGTTT ACGATGAAGA TGTTGGCATT AACTATAGGG AAGTCACTTT TGTTCCTGGT
    TTGTACAAAA TCTTTGATGA GATTCTAGTT AATGCTGCGG ACAACAAACA AAGGGACCCA
    AAAATGTCTT GTATTAGAGT CACAATTGAT CCGGAAAACA ATTTAATTAG TATATGGAAT
    AATGGAAAAG GTATTCCTGT TGTTGAACAC AAAGTTGAAA AGATGTATGT CCCAGCTCTC
    ATATTTGGAC AGCTCCTAAC TTCTAGTAAC TATGATGATG ATGAAAAGAA AGTGACAGGT
    GGTCGAAATG GCTATGGAGC CAAATTGTGT AACATATTCA GTACCAAATT TACTGTGGAA
    ACAGCCAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA TATGGGAAGA
    GCTGGTGAGA TGGAACTCAA GCCCTTCAAT GGAGAAGATT ATACATGTAT CACCTTTCAG
    CCTGATTTGT CTAAGTTTAA AATGCAAAGC CTGGACAAAG ATATTGTTGC ACTAATGGTC
    AGAAGAGCAT ATGATATTGC TGGATCCACC AAAGATGTCA AAGTCTTTCT TAATGGAAAT
    AAACTGCCAG TAAAAGGATT TCGTAGTTAT GTGGACATGT ATTTGAAGGA CAAGTTGGAT
    GAAACTGGTA ACTCCTTGAA AGTAATACAT GAACAAGTAA ACCACAGGTG GGAAGTGTGT
    TTAACTATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTTG TCAACAGCAT TGCTACATCC
    AAGGGTGGCA GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT
    GTGAAGAAGA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTGAA AAATCACATG
    TGGATTTTTG TAAATGCCTT AATTGAAAAC CCAACCTTTG ACTCTCAGAC AAAAGAAAAC
    ATGACTTTAC AACCCAAGAG CTTTGGATCA ACCTGCCAAT TGAGTGAAAA ATTTATCAAA
    GCTGCCATTG GCTGTGGTAT TGTAGAAAGC ATACTAAACT GGGTGAAGTT TAAGGCCCAA
    GTCCAGTTAA ACAAGAAGTG TTCAGCTGTA AAACATAATA GAATCAAGGG AATTCCCAAA
    CTCGATGATG CCAATGATGC AGGGGGCCGA AACTCCACTG AGTGTACGCT TATCCTGACT
    GAGGGAGATT CAGCCAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAA
    TATGGGGTTT TCCCTCTTAG AGGAAAAATA CTCAATGTTC GAGAAGCTTC TCATAAGCAG
    ATCATGGAAA ATGCTGAGAT TAACAATATC ATCAAGATTG TGGGTCTTCA GTACAAGAAA
    AACTATGAAG ATGAAGATTC ATTGAAGACT CTTCGTTATG GGAAGATAAT GATTATGACA
    GATCAGGACC AAGATGGTTC CCACATCAAA GGCTTGCTGA TTAATTTTAT CCATCACAAC
    TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCCAT TGTAAAGGTG
    TCTAAAAACA AGCAAGAAAT GGCATTTTAC AGCCTTCCTG AATTTGAAGA ATGGAAGAGT
    TCTACTCCAA ATCATAAAAA ATGGAAAGTC AAATATTACA AAGGTTTGGG CACCAGCACA
    TCAAAGGAAG CTAAAGAATA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA GTTCAAATAT
    TCTGGTCCTG AAGATGATGC TGCTATCAGC CTGGCCTTTA GCAAAAAACA GATAGATGAT
    CGAAAGGAAT GGTTAACTAA TTTCATGGAG GATAGAAGAC AACGAAAGTT ACTTGGGCTT
    CCTGAGGATT ACTTGTATGG ACAAACTACC ACATATCTGA CATATAATGA CTTCATCAAC
    AAGGAACTTA TCTTGTTCTC AAATTCTGAT AACGAGAGAT CTATCCCTTC TATGGTGGAT
    GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACTTGCT TCAAACGGAA TGACAAGCGA
    GAGGTAAAGG TTGCCCAATT AGCTGGATCA GTGGCTGAAA TGTCTTCTTA TCATCATGGT
    GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ATTTTGTGGG TAGCAATAAT
    CTAAACCTCT TGCAGCCCAT TGGTCAGTTT GGTACCAGGC TACATGGTGG CAAGGATTCT
    GCTAGTCCAC GATACATCTT TACAATGCTC AGCTCTTTGG CTCGATTGTT ATTTCCACCA
    AAAGATGATC ACACGTTGAA GTTTTTATAT GATGACAACC AGCGTGTTGA GCCTGAATGG
    TACATTCCTA TTATTCCCAT GGTGCTGATA AATGGTGCTG AAGGAATCGG TACTGGGTGG
    TCCTGCAAAA TCCCCAACTT TGATGTGCGT GAAATTGTAA ATAACATCAG GCGTTTGATG
    GATGGAGAAG AACCATTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACTATTGAA
    GAACTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTAG CTATTCTTAA TTCTACAACC
    ATTGAAATCT CAGAGCTTCC CATCAGAACA TGGACCCAGA CATACAAAGA ACAAGTTCTA
    GAACCCATGT TGAATGGCAC CGAGAAGACA CCTCCTCTCA TAACAGACTA TAGGGAATAC
    CATACAGATA CCACTGTGAA ATTTGTTGTG AAGATGACTG AAGAAAAACT GGCAGAGGCA
    GAGAGAGTTG GACTACACAA AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCTATG
    GTGCTTTTTG ACCACGTAGG CTGTTTAAAG AAATATGACA CGGTGTTGGA TATTCTAAGA
    GACTTTTTTG AACTCAGACT TAAATATTAT GGATTAAGAA AAGAATGGCT CCTAGGAATG
    CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT
    GGCAAAATAA TCATTGAAAA TAAGCCTAAG AAAGAATTAA TTAAAGTTCT GATTCAGAGG
    GGATATGATT CGGATCCTGT GAAGGCCTGG AAAGAAGCCC AGCAAAAGGT TCCAGATGAA
    GAAGAAAATG AAGAGAGTGA CAACGAAAAG GAAACTGAAA AGAGTGACTC CGTAACAGAT
    TCTGGACCAA CCTTCAACTA TCTTCTTGAT ATGCCCCTTT GGTATTTAAC CAAGGAAAAG
    AAAGATGAAC TCTGCAGGCT AAGAAATGAA AAAGAACAAG AGCTGGACAC ATTAAAAAGA
    AAGAGTCCAT CAGATTTGTG GAAAGAAGAC TTGGCTACAT TTATTGAAGA ATTGGAGGCT
    GTTGAAGCCA AGGAAAAACA AGATGAACAA GTCGGACTTC CTGGGAAAGG GGGGAAGGCC
    AAGGGGAAAA AAACACAAAT GGCTGAAGTT TTGCCTTCTC CACGTGGTCA AAGAGTCATT
    CCACGAATAA CCATAGAAAT GAAAGCAGAG GCAGAAAAGA AAAATAAAAA GAAAATTAAG
    AATGAAAATA CTGAAGGAAG CCCTCAAGAA GATGGTGTGG AACTAGAAGG CCTAAAACAA
    AGATTAGAAA AGAAACAGAA AAGAGAACCA GGTACAAAGA CAAAGAAACA AACTACATTG
    CCATTTAAGC CAATCAAAAA AGGAAAGAAG AGAAATCCCT GGTCTGATTC AGAATCAGAT
    AGGAGCAGTG ACGAAAGTAA TTTTGATGTC CCTCCACGAG AAACAGAGCC ACGGAGAGCA
    GCAACAAAAA CCAAATTCAC AATGGATTTG GATTCAGATG AAGATTTCTC AGATTTTGAT
    GAAAAAACTG ATGATGAAGA TTTTGTCCCA TCAGATGCTA GTCCACCTAA GACCAAAACT
    TCCCCAAAAC CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc99686
    Clone ID Related Accession (Same CDS sequence) XM_016931846.2
    Accession Version XM_016931846.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4095bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product DNA topoisomerase 2-alpha isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036896.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016931846.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAAGTGT CACCATTGCA GCCTGTAAAT GAAAATATGC AAGTCAACAA AATAAAGAAA 
    AATGAAGATG CTAAGAAAAG ACTGTCTGTT GAAAGAATCT ATCAAAAGAA AACACAATTG
    GAACATATTT TGCTCCGCCC AGACACCTAC ATTGGTTCTG TGGAATTAGT GACCCAGCAA
    ATGTGGGTTT ACGATGAAGA TGTTGGCATT AACTATAGGG AAGTCACTTT TGTTCCTGGT
    TTGTACAAAA TCTTTGATGA GATTCTAGTT AATGCTGCGG ACAACAAACA AAGGGACCCA
    AAAATGTCTT GTATTAGAGT CACAATTGAT CCGGAAAACA ATTTAATTAG TATATGGAAT
    AATGGAAAAG GTATTCCTGT TGTTGAACAC AAAGTTGAAA AGATGTATGT CCCAGCTCTC
    ATATTTGGAC AGCTCCTAAC TTCTAGTAAC TATGATGATG ATGAAAAGAA AGTGACAGGT
    GGTCGAAATG GCTATGGAGC CAAATTGTGT AACATATTCA GTACCAAATT TACTGTGGAA
    ACAGCCAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA TATGGGAAGA
    GCTGGTGAGA TGGAACTCAA GCCCTTCAAT GGAGAAGATT ATACATGTAT CACCTTTCAG
    CCTGATTTGT CTAAGTTTAA AATGCAAAGC CTGGACAAAG ATATTGTTGC ACTAATGGTC
    AGAAGAGCAT ATGATATTGC TGGATCCACC AAAGATGTCA AAGTCTTTCT TAATGGAAAT
    AAACTGCCAG TAAAAGGATT TCGTAGTTAT GTGGACATGT ATTTGAAGGA CAAGTTGGAT
    GAAACTGGTA ACTCCTTGAA AGTAATACAT GAACAAGTAA ACCACAGGTG GGAAGTGTGT
    TTAACTATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTTG TCAACAGCAT TGCTACATCC
    AAGGGTGGCA GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT
    GTGAAGAAGA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTGAA AAATCACATG
    TGGATTTTTG TAAATGCCTT AATTGAAAAC CCAACCTTTG ACTCTCAGAC AAAAGAAAAC
    ATGACTTTAC AACCCAAGAG CTTTGGATCA ACCTGCCAAT TGAGTGAAAA ATTTATCAAA
    GCTGCCATTG GCTGTGGTAT TGTAGAAAGC ATACTAAACT GGGTGAAGTT TAAGGCCCAA
    GTCCAGTTAA ACAAGAAGTG TTCAGCTGTA AAACATAATA GAATCAAGGG AATTCCCAAA
    CTCGATGATG CCAATGATGC AGGGGGCCGA AACTCCACTG AGTGTACGCT TATCCTGACT
    GAGGGAGATT CAGCCAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAA
    TATGGGGTTT TCCCTCTTAG AGGAAAAATA CTCAATGTTC GAGAAGCTTC TCATAAGCAG
    ATCATGGAAA ATGCTGAGAT TAACAATATC ATCAAGATTG TGGGTCTTCA GTACAAGAAA
    AACTATGAAG ATGAAGATTC ATTGAAGACT CTTCGTTATG GGAAGATAAT GATTATGACA
    GATCAGGACC AAGATGGTTC CCACATCAAA GGCTTGCTGA TTAATTTTAT CCATCACAAC
    TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCCAT TGTAAAGGTG
    TCTAAAAACA AGCAAGAAAT GGCATTTTAC AGCCTTCCTG AATTTGAAGA ATGGAAGAGT
    TCTACTCCAA ATCATAAAAA ATGGAAAGTC AAATATTACA AAGGTTTGGG CACCAGCACA
    TCAAAGGAAG CTAAAGAATA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA GTTCAAATAT
    TCTGGTCCTG AAGATGATGC TGCTATCAGC CTGGCCTTTA GCAAAAAACA GATAGATGAT
    CGAAAGGAAT GGTTAACTAA TTTCATGGAG GATAGAAGAC AACGAAAGTT ACTTGGGCTT
    CCTGAGGATT ACTTGTATGG ACAAACTACC ACATATCTGA CATATAATGA CTTCATCAAC
    AAGGAACTTA TCTTGTTCTC AAATTCTGAT AACGAGAGAT CTATCCCTTC TATGGTGGAT
    GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACTTGCT TCAAACGGAA TGACAAGCGA
    GAGGTAAAGG TTGCCCAATT AGCTGGATCA GTGGCTGAAA TGTCTTCTTA TCATCATGGT
    GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ATTTTGTGGG TAGCAATAAT
    CTAAACCTCT TGCAGCCCAT TGGTCAGTTT GGTACCAGGC TACATGGTGG CAAGGATTCT
    GCTAGTCCAC GATACATCTT TACAATGCTC AGCTCTTTGG CTCGATTGTT ATTTCCACCA
    AAAGATGATC ACACGTTGAA GTTTTTATAT GATGACAACC AGCGTGTTGA GCCTGAATGG
    TACATTCCTA TTATTCCCAT GGTGCTGATA AATGGTGCTG AAGGAATCGG TACTGGGTGG
    TCCTGCAAAA TCCCCAACTT TGATGTGCGT GAAATTGTAA ATAACATCAG GCGTTTGATG
    GATGGAGAAG AACCATTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACTATTGAA
    GAACTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTAG CTATTCTTAA TTCTACAACC
    ATTGAAATCT CAGAGCTTCC CATCAGAACA TGGACCCAGA CATACAAAGA ACAAGTTCTA
    GAACCCATGT TGAATGGCAC CGAGAAGACA CCTCCTCTCA TAACAGACTA TAGGGAATAC
    CATACAGATA CCACTGTGAA ATTTGTTGTG AAGATGACTG AAGAAAAACT GGCAGAGGCA
    GAGAGAGTTG GACTACACAA AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCTATG
    GTGCTTTTTG ACCACGTAGG CTGTTTAAAG AAATATGACA CGGTGTTGGA TATTCTAAGA
    GACTTTTTTG AACTCAGACT TAAATATTAT GGATTAAGAA AAGAATGGCT CCTAGGAATG
    CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT
    GGCAAAATAA TCATTGAAAA TAAGCCTAAG AAAGAATTAA TTAAAGTTCT GATTCAGAGG
    GGATATGATT CGGATCCTGT GAAGGCCTGG AAAGAAGCCC AGCAAAAGGT TCCAGATGAA
    GAAGAAAATG AAGAGAGTGA CAACGAAAAG GAAACTGAAA AGAGTGACTC CGTAACAGAT
    TCTGGACCAA CCTTCAACTA TCTTCTTGAT ATGCCCCTTT GGTATTTAAC CAAGGAAAAG
    AAAGATGAAC TCTGCAGGCT AAGAAATGAA AAAGAACAAG AGCTGGACAC ATTAAAAAGA
    AAGAGTCCAT CAGATTTGTG GAAAGAAGAC TTGGCTACAT TTATTGAAGA ATTGGAGGCT
    GTTGAAGCCA AGGAAAAACA AGATGAACAA GTCGGACTTC CTGGGAAAGG GGGGAAGGCC
    AAGGGGAAAA AAACACAAAT GGCTGAAGTT TTGCCTTCTC CACGTGGTCA AAGAGTCATT
    CCACGAATAA CCATAGAAAT GAAAGCAGAG GCAGAAAAGA AAAATAAAAA GAAAATTAAG
    AATGAAAATA CTGAAGGAAG CCCTCAAGAA GATGGTGTGG AACTAGAAGG CCTAAAACAA
    AGATTAGAAA AGAAACAGAA AAGAGAACCA GGTACAAAGA CAAAGAAACA AACTACATTG
    CCATTTAAGC CAATCAAAAA AGGAAAGAAG AGAAATCCCT GGTCTGATTC AGAATCAGAT
    AGGAGCAGTG ACGAAAGTAA TTTTGATGTC CCTCCACGAG AAACAGAGCC ACGGAGAGCA
    GCAACAAAAA CCAAATTCAC AATGGATTTG GATTCAGATG AAGATTTCTC AGATTTTGAT
    GAAAAAACTG ATGATGAAGA TTTTGTCCCA TCAGATGCTA GTCCACCTAA GACCAAAACT
    TCCCCAAAAC CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016787335.1
    CDS152..4246
    Translation

    Target ORF information:

    RefSeq Version XM_016931846.2
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes DNA topoisomerase II alpha (TOP2A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016931846.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAAGTGT CACCATTGCA GCCTGTAAAT GAAAATATGC AAGTCAACAA AATAAAGAAA 
    AATGAAGATG CTAAGAAAAG ACTGTCTGTT GAAAGAATCT ATCAAAAGAA AACACAATTG
    GAACATATTT TGCTCCGCCC AGACACCTAC ATTGGTTCTG TGGAATTAGT GACCCAGCAA
    ATGTGGGTTT ACGATGAAGA TGTTGGCATT AACTATAGGG AAGTCACTTT TGTTCCTGGT
    TTGTACAAAA TCTTTGATGA GATTCTAGTT AATGCTGCGG ACAACAAACA AAGGGACCCA
    AAAATGTCTT GTATTAGAGT CACAATTGAT CCGGAAAACA ATTTAATTAG TATATGGAAT
    AATGGAAAAG GTATTCCTGT TGTTGAACAC AAAGTTGAAA AGATGTATGT CCCAGCTCTC
    ATATTTGGAC AGCTCCTAAC TTCTAGTAAC TATGATGATG ATGAAAAGAA AGTGACAGGT
    GGTCGAAATG GCTATGGAGC CAAATTGTGT AACATATTCA GTACCAAATT TACTGTGGAA
    ACAGCCAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA TATGGGAAGA
    GCTGGTGAGA TGGAACTCAA GCCCTTCAAT GGAGAAGATT ATACATGTAT CACCTTTCAG
    CCTGATTTGT CTAAGTTTAA AATGCAAAGC CTGGACAAAG ATATTGTTGC ACTAATGGTC
    AGAAGAGCAT ATGATATTGC TGGATCCACC AAAGATGTCA AAGTCTTTCT TAATGGAAAT
    AAACTGCCAG TAAAAGGATT TCGTAGTTAT GTGGACATGT ATTTGAAGGA CAAGTTGGAT
    GAAACTGGTA ACTCCTTGAA AGTAATACAT GAACAAGTAA ACCACAGGTG GGAAGTGTGT
    TTAACTATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTTG TCAACAGCAT TGCTACATCC
    AAGGGTGGCA GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT
    GTGAAGAAGA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTGAA AAATCACATG
    TGGATTTTTG TAAATGCCTT AATTGAAAAC CCAACCTTTG ACTCTCAGAC AAAAGAAAAC
    ATGACTTTAC AACCCAAGAG CTTTGGATCA ACCTGCCAAT TGAGTGAAAA ATTTATCAAA
    GCTGCCATTG GCTGTGGTAT TGTAGAAAGC ATACTAAACT GGGTGAAGTT TAAGGCCCAA
    GTCCAGTTAA ACAAGAAGTG TTCAGCTGTA AAACATAATA GAATCAAGGG AATTCCCAAA
    CTCGATGATG CCAATGATGC AGGGGGCCGA AACTCCACTG AGTGTACGCT TATCCTGACT
    GAGGGAGATT CAGCCAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAA
    TATGGGGTTT TCCCTCTTAG AGGAAAAATA CTCAATGTTC GAGAAGCTTC TCATAAGCAG
    ATCATGGAAA ATGCTGAGAT TAACAATATC ATCAAGATTG TGGGTCTTCA GTACAAGAAA
    AACTATGAAG ATGAAGATTC ATTGAAGACT CTTCGTTATG GGAAGATAAT GATTATGACA
    GATCAGGACC AAGATGGTTC CCACATCAAA GGCTTGCTGA TTAATTTTAT CCATCACAAC
    TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCCAT TGTAAAGGTG
    TCTAAAAACA AGCAAGAAAT GGCATTTTAC AGCCTTCCTG AATTTGAAGA ATGGAAGAGT
    TCTACTCCAA ATCATAAAAA ATGGAAAGTC AAATATTACA AAGGTTTGGG CACCAGCACA
    TCAAAGGAAG CTAAAGAATA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA GTTCAAATAT
    TCTGGTCCTG AAGATGATGC TGCTATCAGC CTGGCCTTTA GCAAAAAACA GATAGATGAT
    CGAAAGGAAT GGTTAACTAA TTTCATGGAG GATAGAAGAC AACGAAAGTT ACTTGGGCTT
    CCTGAGGATT ACTTGTATGG ACAAACTACC ACATATCTGA CATATAATGA CTTCATCAAC
    AAGGAACTTA TCTTGTTCTC AAATTCTGAT AACGAGAGAT CTATCCCTTC TATGGTGGAT
    GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACTTGCT TCAAACGGAA TGACAAGCGA
    GAGGTAAAGG TTGCCCAATT AGCTGGATCA GTGGCTGAAA TGTCTTCTTA TCATCATGGT
    GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ATTTTGTGGG TAGCAATAAT
    CTAAACCTCT TGCAGCCCAT TGGTCAGTTT GGTACCAGGC TACATGGTGG CAAGGATTCT
    GCTAGTCCAC GATACATCTT TACAATGCTC AGCTCTTTGG CTCGATTGTT ATTTCCACCA
    AAAGATGATC ACACGTTGAA GTTTTTATAT GATGACAACC AGCGTGTTGA GCCTGAATGG
    TACATTCCTA TTATTCCCAT GGTGCTGATA AATGGTGCTG AAGGAATCGG TACTGGGTGG
    TCCTGCAAAA TCCCCAACTT TGATGTGCGT GAAATTGTAA ATAACATCAG GCGTTTGATG
    GATGGAGAAG AACCATTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACTATTGAA
    GAACTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTAG CTATTCTTAA TTCTACAACC
    ATTGAAATCT CAGAGCTTCC CATCAGAACA TGGACCCAGA CATACAAAGA ACAAGTTCTA
    GAACCCATGT TGAATGGCAC CGAGAAGACA CCTCCTCTCA TAACAGACTA TAGGGAATAC
    CATACAGATA CCACTGTGAA ATTTGTTGTG AAGATGACTG AAGAAAAACT GGCAGAGGCA
    GAGAGAGTTG GACTACACAA AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCTATG
    GTGCTTTTTG ACCACGTAGG CTGTTTAAAG AAATATGACA CGGTGTTGGA TATTCTAAGA
    GACTTTTTTG AACTCAGACT TAAATATTAT GGATTAAGAA AAGAATGGCT CCTAGGAATG
    CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT
    GGCAAAATAA TCATTGAAAA TAAGCCTAAG AAAGAATTAA TTAAAGTTCT GATTCAGAGG
    GGATATGATT CGGATCCTGT GAAGGCCTGG AAAGAAGCCC AGCAAAAGGT TCCAGATGAA
    GAAGAAAATG AAGAGAGTGA CAACGAAAAG GAAACTGAAA AGAGTGACTC CGTAACAGAT
    TCTGGACCAA CCTTCAACTA TCTTCTTGAT ATGCCCCTTT GGTATTTAAC CAAGGAAAAG
    AAAGATGAAC TCTGCAGGCT AAGAAATGAA AAAGAACAAG AGCTGGACAC ATTAAAAAGA
    AAGAGTCCAT CAGATTTGTG GAAAGAAGAC TTGGCTACAT TTATTGAAGA ATTGGAGGCT
    GTTGAAGCCA AGGAAAAACA AGATGAACAA GTCGGACTTC CTGGGAAAGG GGGGAAGGCC
    AAGGGGAAAA AAACACAAAT GGCTGAAGTT TTGCCTTCTC CACGTGGTCA AAGAGTCATT
    CCACGAATAA CCATAGAAAT GAAAGCAGAG GCAGAAAAGA AAAATAAAAA GAAAATTAAG
    AATGAAAATA CTGAAGGAAG CCCTCAAGAA GATGGTGTGG AACTAGAAGG CCTAAAACAA
    AGATTAGAAA AGAAACAGAA AAGAGAACCA GGTACAAAGA CAAAGAAACA AACTACATTG
    CCATTTAAGC CAATCAAAAA AGGAAAGAAG AGAAATCCCT GGTCTGATTC AGAATCAGAT
    AGGAGCAGTG ACGAAAGTAA TTTTGATGTC CCTCCACGAG AAACAGAGCC ACGGAGAGCA
    GCAACAAAAA CCAAATTCAC AATGGATTTG GATTCAGATG AAGATTTCTC AGATTTTGAT
    GAAAAAACTG ATGATGAAGA TTTTGTCCCA TCAGATGCTA GTCCACCTAA GACCAAAACT
    TCCCCAAAAC CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc72521
    Clone ID Related Accession (Same CDS sequence) XM_016931845.1
    Accession Version XM_016931845.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4596bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product DNA topoisomerase 2-alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006484.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAAGTGT CACCATTGCA GCCCGTAAAT GAAAATATGC AAGTCAACAA AATAAAGAAA 
    AATGAAGATG CTAAGAAAAG ACTGTCTGTT GAAAGAATCT ATCAAAAGAA AACACAATTG
    GAACATATTT TGCTCCGCCC AGACACCTAC ATTGGTTCTG TGGAATTAGT GACCCAGCAA
    ATGTGGGTTT ACGATGAAGA TGTTGGCATT AACTATAGGG AAGTCACTTT TGTTCCTGGT
    TTGTACAAAA TCTTTGATGA GATTCTAGTT AATGCTGCGG ACAACAAACA AAGGGACCCA
    AAAATGTCTT GTATTAGAGT CACAATTGAT CCGGAAAACA ATTTAATTAG TATATGGAAT
    AATGGAAAAG GTATTCCTGT TGTTGAACAC AAAGTTGAAA AGATGTATGT CCCAGCTCTC
    ATATTTGGAC AGCTCCTAAC TTCTAGTAAC TATGATGATG ATGAAAAGAA AGTGACAGGT
    GGTCGAAATG GCTATGGAGC CAAATTGTGT AACATATTCA GTACCAAATT TACTGTGGAA
    ACAGCCAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA TATGGGAAGA
    GCTGGTGAGA TGGAACTCAA GCCCTTCAAT GGAGAAGATT ATACATGTAT CACCTTTCAG
    CCTGATTTGT CTAAGTTTAA AATGCAAAGC CTGGACAAAG ATATTGTTGC ACTAATGGTC
    AGAAGAGCAT ATGATATTGC TGGATCCACC AAAGATGTCA AAGTCTTTCT TAATGGAAAT
    AAACTGCCAG TAAAAGGATT TCGTAGTTAT GTGGACATGT ATTTGAAGGA CAAGTTGGAT
    GAAACTGGTA ACTCCTTGAA AGTAATACAT GAACAAGTAA ACCACAGGTG GGAAGTGTGT
    TTAACTATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTTG TCAACAGCAT TGCTACATCC
    AAGGGTGGCA GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT
    GTGAAGAAGA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTGAA AAATCACATG
    TGGATTTTTG TAAATGCCTT AATTGAAAAC CCAACCTTTG ACTCTCAGAC AAAAGAAAAC
    ATGACTTTAC AACCCAAGAG CTTTGGATCA ACCTGCCAAT TGAGTGAAAA ATTTATCAAA
    GCTGCCATTG GCTGTGGTAT TGTAGAAAGC ATACTAAACT GGGTGAAGTT TAAGGCCCAA
    GTCCAGTTAA ACAAGAAGTG TTCAGCTGTA AAACATAATA GAATCAAGGG AATTCCCAAA
    CTCGATGATG CCAATGATGC AGGGGGCCGA AACTCCACTG AGTGTACGCT TATCCTGACT
    GAGGGAGATT CAGCCAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAA
    TATGGGGTTT TCCCTCTTAG AGGAAAAATA CTCAATGTTC GAGAAGCTTC TCATAAGCAG
    ATCATGGAAA ATGCTGAGAT TAACAATATC ATCAAGATTG TGGGTCTTCA GTACAAGAAA
    AACTATGAAG ATGAAGATTC ATTGAAGACT CTTCGTTATG GGAAGATAAT GATTATGACA
    GATCAGGACC AAGATGGTTC CCACATCAAA GGCTTGCTGA TTAATTTTAT CCATCACAAC
    TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCCAT TGTAAAGGTG
    TCTAAAAACA AGCAAGAAAT GGCATTTTAC AGCCTTCCTG AATTTGAAGA ATGGAAGAGT
    TCTACTCCAA ATCATAAAAA ATGGAAAGTC AAATATTACA AAGGTTTGGG CACCAGCACA
    TCAAAGGAAG CTAAAGAATA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA GTTCAAATAT
    TCTGGTCCTG AAGATGATGC TGCTATCAGC CTGGCCTTTA GCAAAAAACA GATAGATGAT
    CGAAAGGAAT GGTTAACTAA TTTCATGGAG GATAGAAGAC AACGAAAGTT ACTTGGGCTT
    CCTGAGGATT ACTTGTATGG ACAAACTACC ACATATCTGA CATATAATGA CTTCATCAAC
    AAGGAACTTA TCTTGTTCTC AAATTCTGAT AACGAGAGAT CTATCCCTTC TATGGTGGAT
    GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACTTGCT TCAAACGGAA TGACAAGCGA
    GAGGTAAAGG TTGCCCAATT AGCTGGATCA GTGGCTGAAA TGTCTTCTTA TCATCATGGT
    GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ATTTTGTGGG TAGCAATAAT
    CTAAACCTCT TGCAGCCCAT TGGTCAGTTT GGTACCAGGC TACATGGTGG CAAGGATTCT
    GCTAGTCCAC GATACATCTT TACAATGCTC AGCTCTTTGG CTCGATTGTT ATTTCCACCA
    AAAGATGATC ACACGTTGAA GTTTTTATAT GATGACAACC AGCGTGTTGA GCCTGAATGG
    TACATTCCTA TTATTCCCAT GGTGCTGATA AATGGTGCTG AAGGAATCGG TACTGGGTGG
    TCCTGCAAAA TCCCCAACTT TGATGTGCGT GAAATTGTAA ATAACATCAG GCGTTTGATG
    GATGGAGAAG AACCATTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACTATTGAA
    GAACTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTAG CTATTCTTAA TTCTACAACC
    ATTGAAATCT CAGAGCTTCC CATCAGAACA TGGACCCAGA CATACAAAGA ACAAGTTCTA
    GAACCCATGT TGAATGGCAC CGAGAAGACA CCTCCTCTCA TAACAGACTA TAGGGAATAC
    CATACAGATA CCACTGTGAA ATTTGTTGTG AAGATGACTG AAGAAAAACT GGCAGAGGCA
    GAGAGAGTTG GACTACACAA AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCTATG
    GTGCTTTTTG ACCACGTAGG CTGTTTAAAG AAATATGACA CGGTGTTGGA TATTCTAAGA
    GACTTTTTTG AACTCAGACT TAAATATTAT GGATTAAGAA AAGAATGGCT CCTAGGAATG
    CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT
    GGCAAAATAA TCATTGAAAA TAAGCCTAAG AAAGAATTAA TTAAAGTTCT GATTCAGAGG
    GGATATGATT CGGATCCTGT GAAGGCCTGG AAAGAAGCCC AGCAAAAGGT TCCAGATGAA
    GAAGAAAATG AAGAGAGTGA CAACGAAAAG GAAACTGAAA AGAGTGACTC CGTAACAGAT
    TCTGGACCAA CCTTCAACTA TCTTCTTGAT ATGCCCCTTT GGTATTTAAC CAAGGAAAAG
    AAAGATGAAC TCTGCAGGCT AAGAAATGAA AAAGAACAAG AGCTGGACAC ATTAAAAAGA
    AAGAGTCCAT CAGATTTGTG GAAAGAAGAC TTGGCTACAT TTATTGAAGA ATTGGAGGCT
    GTTGAAGCCA AGGAAAAACA AGATGAACAA GTCGGACTTC CTGGGAAAGG GGGGAAGGCC
    AAGGGGAAAA AAACACAAAT GGCTGAAGTT TTGCCTTCTC CACGTGGTCA AAGAGTCATT
    CCACGAATAA CCATAGAAAT GAAAGCAGAG GCAGAAAAGA AAAATAAAAA GAAAATTAAG
    AATGAAAATA CTGAAGGAAG CCCTCAAGAA GATGGTGTGG AACTAGAAGG CCTAAAACAA
    AGATTAGAAA AGAAACAGAA AAGAGAACCA GGTACAAAGA CAAAGAAACA AACTACATTG
    CCATTTAAGC CAATCAAAAA AGGAAAGAAG AGAAATCCCT GGTCTGATTC AGAATCAGAT
    AGGAGCAGTG ACGAAAGTAA TTTTGATGTC CCTCCACGAG AAACAGAGCC ACGGAGAGCA
    GCAACAAAAA CCAAATTCAC AATGGATTTG GATTCAGATG AAGATTTCTC AGATTTTGAT
    GAAAAAACTG ATGATGAAGA TTTTGTCCCA TCAGATGCTA GTCCACCTAA GACCAAAACT
    TCCCCAAAAC TTAGTAACAA AGAACTGAAA CCACAGAAAA GTGTCGTGTC AGACCTTGAA
    GCTAATGATG CTAAGGACAG TGTACCACTG TCTTCAAGCC CTCCTGCTAC ACATTTCCCA
    GATGAAACTG AAATTACAAA CCCAGTTCCT AAAAAGAATG TGACAGTGAA GAAGACAGCA
    GCAAAAAGTC AGTCTTCCAC CTCCACTACC GGTGCCAAAA AAAGGGCTGC CCCAAAAGGA
    ACTAAAAGGG ATCCAGCTTT GAATTCTGGT GTCTCTCAGA AGCCTGATCC TGCCAAAACC
    AAGAATCGCC GCAAAAGGAA GCCATCCACT TCTGATGATT CTGACTCTAA TTTTGAGAAA
    ATTGTTTCGA AAGCAGTCAC AAGCAAGAAA TCCAAGGGGG AGAGTGATGA CTTCCATATG
    GACTTTGACT CAGCTGTGGC TCCTCGGGCA AAATCTGTAC GGGCAAAGAA ACCTATAAAG
    TACCTGGAAG AGTCAGATGA AGATGATCTG TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016787334.1
    CDS160..4755
    Translation

    Target ORF information:

    RefSeq Version XM_016931845.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes topoisomerase (DNA) II alpha (TOP2A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016931845.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAAGTGT CACCATTGCA GCCCGTAAAT GAAAATATGC AAGTCAACAA AATAAAGAAA 
    AATGAAGATG CTAAGAAAAG ACTGTCTGTT GAAAGAATCT ATCAAAAGAA AACACAATTG
    GAACATATTT TGCTCCGCCC AGACACCTAC ATTGGTTCTG TGGAATTAGT GACCCAGCAA
    ATGTGGGTTT ACGATGAAGA TGTTGGCATT AACTATAGGG AAGTCACTTT TGTTCCTGGT
    TTGTACAAAA TCTTTGATGA GATTCTAGTT AATGCTGCGG ACAACAAACA AAGGGACCCA
    AAAATGTCTT GTATTAGAGT CACAATTGAT CCGGAAAACA ATTTAATTAG TATATGGAAT
    AATGGAAAAG GTATTCCTGT TGTTGAACAC AAAGTTGAAA AGATGTATGT CCCAGCTCTC
    ATATTTGGAC AGCTCCTAAC TTCTAGTAAC TATGATGATG ATGAAAAGAA AGTGACAGGT
    GGTCGAAATG GCTATGGAGC CAAATTGTGT AACATATTCA GTACCAAATT TACTGTGGAA
    ACAGCCAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA TATGGGAAGA
    GCTGGTGAGA TGGAACTCAA GCCCTTCAAT GGAGAAGATT ATACATGTAT CACCTTTCAG
    CCTGATTTGT CTAAGTTTAA AATGCAAAGC CTGGACAAAG ATATTGTTGC ACTAATGGTC
    AGAAGAGCAT ATGATATTGC TGGATCCACC AAAGATGTCA AAGTCTTTCT TAATGGAAAT
    AAACTGCCAG TAAAAGGATT TCGTAGTTAT GTGGACATGT ATTTGAAGGA CAAGTTGGAT
    GAAACTGGTA ACTCCTTGAA AGTAATACAT GAACAAGTAA ACCACAGGTG GGAAGTGTGT
    TTAACTATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTTG TCAACAGCAT TGCTACATCC
    AAGGGTGGCA GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT
    GTGAAGAAGA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTGAA AAATCACATG
    TGGATTTTTG TAAATGCCTT AATTGAAAAC CCAACCTTTG ACTCTCAGAC AAAAGAAAAC
    ATGACTTTAC AACCCAAGAG CTTTGGATCA ACCTGCCAAT TGAGTGAAAA ATTTATCAAA
    GCTGCCATTG GCTGTGGTAT TGTAGAAAGC ATACTAAACT GGGTGAAGTT TAAGGCCCAA
    GTCCAGTTAA ACAAGAAGTG TTCAGCTGTA AAACATAATA GAATCAAGGG AATTCCCAAA
    CTCGATGATG CCAATGATGC AGGGGGCCGA AACTCCACTG AGTGTACGCT TATCCTGACT
    GAGGGAGATT CAGCCAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAA
    TATGGGGTTT TCCCTCTTAG AGGAAAAATA CTCAATGTTC GAGAAGCTTC TCATAAGCAG
    ATCATGGAAA ATGCTGAGAT TAACAATATC ATCAAGATTG TGGGTCTTCA GTACAAGAAA
    AACTATGAAG ATGAAGATTC ATTGAAGACT CTTCGTTATG GGAAGATAAT GATTATGACA
    GATCAGGACC AAGATGGTTC CCACATCAAA GGCTTGCTGA TTAATTTTAT CCATCACAAC
    TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCCAT TGTAAAGGTG
    TCTAAAAACA AGCAAGAAAT GGCATTTTAC AGCCTTCCTG AATTTGAAGA ATGGAAGAGT
    TCTACTCCAA ATCATAAAAA ATGGAAAGTC AAATATTACA AAGGTTTGGG CACCAGCACA
    TCAAAGGAAG CTAAAGAATA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA GTTCAAATAT
    TCTGGTCCTG AAGATGATGC TGCTATCAGC CTGGCCTTTA GCAAAAAACA GATAGATGAT
    CGAAAGGAAT GGTTAACTAA TTTCATGGAG GATAGAAGAC AACGAAAGTT ACTTGGGCTT
    CCTGAGGATT ACTTGTATGG ACAAACTACC ACATATCTGA CATATAATGA CTTCATCAAC
    AAGGAACTTA TCTTGTTCTC AAATTCTGAT AACGAGAGAT CTATCCCTTC TATGGTGGAT
    GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACTTGCT TCAAACGGAA TGACAAGCGA
    GAGGTAAAGG TTGCCCAATT AGCTGGATCA GTGGCTGAAA TGTCTTCTTA TCATCATGGT
    GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ATTTTGTGGG TAGCAATAAT
    CTAAACCTCT TGCAGCCCAT TGGTCAGTTT GGTACCAGGC TACATGGTGG CAAGGATTCT
    GCTAGTCCAC GATACATCTT TACAATGCTC AGCTCTTTGG CTCGATTGTT ATTTCCACCA
    AAAGATGATC ACACGTTGAA GTTTTTATAT GATGACAACC AGCGTGTTGA GCCTGAATGG
    TACATTCCTA TTATTCCCAT GGTGCTGATA AATGGTGCTG AAGGAATCGG TACTGGGTGG
    TCCTGCAAAA TCCCCAACTT TGATGTGCGT GAAATTGTAA ATAACATCAG GCGTTTGATG
    GATGGAGAAG AACCATTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACTATTGAA
    GAACTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTAG CTATTCTTAA TTCTACAACC
    ATTGAAATCT CAGAGCTTCC CATCAGAACA TGGACCCAGA CATACAAAGA ACAAGTTCTA
    GAACCCATGT TGAATGGCAC CGAGAAGACA CCTCCTCTCA TAACAGACTA TAGGGAATAC
    CATACAGATA CCACTGTGAA ATTTGTTGTG AAGATGACTG AAGAAAAACT GGCAGAGGCA
    GAGAGAGTTG GACTACACAA AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCTATG
    GTGCTTTTTG ACCACGTAGG CTGTTTAAAG AAATATGACA CGGTGTTGGA TATTCTAAGA
    GACTTTTTTG AACTCAGACT TAAATATTAT GGATTAAGAA AAGAATGGCT CCTAGGAATG
    CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT
    GGCAAAATAA TCATTGAAAA TAAGCCTAAG AAAGAATTAA TTAAAGTTCT GATTCAGAGG
    GGATATGATT CGGATCCTGT GAAGGCCTGG AAAGAAGCCC AGCAAAAGGT TCCAGATGAA
    GAAGAAAATG AAGAGAGTGA CAACGAAAAG GAAACTGAAA AGAGTGACTC CGTAACAGAT
    TCTGGACCAA CCTTCAACTA TCTTCTTGAT ATGCCCCTTT GGTATTTAAC CAAGGAAAAG
    AAAGATGAAC TCTGCAGGCT AAGAAATGAA AAAGAACAAG AGCTGGACAC ATTAAAAAGA
    AAGAGTCCAT CAGATTTGTG GAAAGAAGAC TTGGCTACAT TTATTGAAGA ATTGGAGGCT
    GTTGAAGCCA AGGAAAAACA AGATGAACAA GTCGGACTTC CTGGGAAAGG GGGGAAGGCC
    AAGGGGAAAA AAACACAAAT GGCTGAAGTT TTGCCTTCTC CACGTGGTCA AAGAGTCATT
    CCACGAATAA CCATAGAAAT GAAAGCAGAG GCAGAAAAGA AAAATAAAAA GAAAATTAAG
    AATGAAAATA CTGAAGGAAG CCCTCAAGAA GATGGTGTGG AACTAGAAGG CCTAAAACAA
    AGATTAGAAA AGAAACAGAA AAGAGAACCA GGTACAAAGA CAAAGAAACA AACTACATTG
    CCATTTAAGC CAATCAAAAA AGGAAAGAAG AGAAATCCCT GGTCTGATTC AGAATCAGAT
    AGGAGCAGTG ACGAAAGTAA TTTTGATGTC CCTCCACGAG AAACAGAGCC ACGGAGAGCA
    GCAACAAAAA CCAAATTCAC AATGGATTTG GATTCAGATG AAGATTTCTC AGATTTTGAT
    GAAAAAACTG ATGATGAAGA TTTTGTCCCA TCAGATGCTA GTCCACCTAA GACCAAAACT
    TCCCCAAAAC TTAGTAACAA AGAACTGAAA CCACAGAAAA GTGTCGTGTC AGACCTTGAA
    GCTAATGATG CTAAGGACAG TGTACCACTG TCTTCAAGCC CTCCTGCTAC ACATTTCCCA
    GATGAAACTG AAATTACAAA CCCAGTTCCT AAAAAGAATG TGACAGTGAA GAAGACAGCA
    GCAAAAAGTC AGTCTTCCAC CTCCACTACC GGTGCCAAAA AAAGGGCTGC CCCAAAAGGA
    ACTAAAAGGG ATCCAGCTTT GAATTCTGGT GTCTCTCAGA AGCCTGATCC TGCCAAAACC
    AAGAATCGCC GCAAAAGGAA GCCATCCACT TCTGATGATT CTGACTCTAA TTTTGAGAAA
    ATTGTTTCGA AAGCAGTCAC AAGCAAGAAA TCCAAGGGGG AGAGTGATGA CTTCCATATG
    GACTTTGACT CAGCTGTGGC TCCTCGGGCA AAATCTGTAC GGGCAAAGAA ACCTATAAAG
    TACCTGGAAG AGTCAGATGA AGATGATCTG TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc99685
    Clone ID Related Accession (Same CDS sequence) XM_016931845.2
    Accession Version XM_016931845.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4596bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product DNA topoisomerase 2-alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036896.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016931845.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAAGTGT CACCATTGCA GCCTGTAAAT GAAAATATGC AAGTCAACAA AATAAAGAAA 
    AATGAAGATG CTAAGAAAAG ACTGTCTGTT GAAAGAATCT ATCAAAAGAA AACACAATTG
    GAACATATTT TGCTCCGCCC AGACACCTAC ATTGGTTCTG TGGAATTAGT GACCCAGCAA
    ATGTGGGTTT ACGATGAAGA TGTTGGCATT AACTATAGGG AAGTCACTTT TGTTCCTGGT
    TTGTACAAAA TCTTTGATGA GATTCTAGTT AATGCTGCGG ACAACAAACA AAGGGACCCA
    AAAATGTCTT GTATTAGAGT CACAATTGAT CCGGAAAACA ATTTAATTAG TATATGGAAT
    AATGGAAAAG GTATTCCTGT TGTTGAACAC AAAGTTGAAA AGATGTATGT CCCAGCTCTC
    ATATTTGGAC AGCTCCTAAC TTCTAGTAAC TATGATGATG ATGAAAAGAA AGTGACAGGT
    GGTCGAAATG GCTATGGAGC CAAATTGTGT AACATATTCA GTACCAAATT TACTGTGGAA
    ACAGCCAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA TATGGGAAGA
    GCTGGTGAGA TGGAACTCAA GCCCTTCAAT GGAGAAGATT ATACATGTAT CACCTTTCAG
    CCTGATTTGT CTAAGTTTAA AATGCAAAGC CTGGACAAAG ATATTGTTGC ACTAATGGTC
    AGAAGAGCAT ATGATATTGC TGGATCCACC AAAGATGTCA AAGTCTTTCT TAATGGAAAT
    AAACTGCCAG TAAAAGGATT TCGTAGTTAT GTGGACATGT ATTTGAAGGA CAAGTTGGAT
    GAAACTGGTA ACTCCTTGAA AGTAATACAT GAACAAGTAA ACCACAGGTG GGAAGTGTGT
    TTAACTATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTTG TCAACAGCAT TGCTACATCC
    AAGGGTGGCA GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT
    GTGAAGAAGA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTGAA AAATCACATG
    TGGATTTTTG TAAATGCCTT AATTGAAAAC CCAACCTTTG ACTCTCAGAC AAAAGAAAAC
    ATGACTTTAC AACCCAAGAG CTTTGGATCA ACCTGCCAAT TGAGTGAAAA ATTTATCAAA
    GCTGCCATTG GCTGTGGTAT TGTAGAAAGC ATACTAAACT GGGTGAAGTT TAAGGCCCAA
    GTCCAGTTAA ACAAGAAGTG TTCAGCTGTA AAACATAATA GAATCAAGGG AATTCCCAAA
    CTCGATGATG CCAATGATGC AGGGGGCCGA AACTCCACTG AGTGTACGCT TATCCTGACT
    GAGGGAGATT CAGCCAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAA
    TATGGGGTTT TCCCTCTTAG AGGAAAAATA CTCAATGTTC GAGAAGCTTC TCATAAGCAG
    ATCATGGAAA ATGCTGAGAT TAACAATATC ATCAAGATTG TGGGTCTTCA GTACAAGAAA
    AACTATGAAG ATGAAGATTC ATTGAAGACT CTTCGTTATG GGAAGATAAT GATTATGACA
    GATCAGGACC AAGATGGTTC CCACATCAAA GGCTTGCTGA TTAATTTTAT CCATCACAAC
    TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCCAT TGTAAAGGTG
    TCTAAAAACA AGCAAGAAAT GGCATTTTAC AGCCTTCCTG AATTTGAAGA ATGGAAGAGT
    TCTACTCCAA ATCATAAAAA ATGGAAAGTC AAATATTACA AAGGTTTGGG CACCAGCACA
    TCAAAGGAAG CTAAAGAATA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA GTTCAAATAT
    TCTGGTCCTG AAGATGATGC TGCTATCAGC CTGGCCTTTA GCAAAAAACA GATAGATGAT
    CGAAAGGAAT GGTTAACTAA TTTCATGGAG GATAGAAGAC AACGAAAGTT ACTTGGGCTT
    CCTGAGGATT ACTTGTATGG ACAAACTACC ACATATCTGA CATATAATGA CTTCATCAAC
    AAGGAACTTA TCTTGTTCTC AAATTCTGAT AACGAGAGAT CTATCCCTTC TATGGTGGAT
    GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACTTGCT TCAAACGGAA TGACAAGCGA
    GAGGTAAAGG TTGCCCAATT AGCTGGATCA GTGGCTGAAA TGTCTTCTTA TCATCATGGT
    GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ATTTTGTGGG TAGCAATAAT
    CTAAACCTCT TGCAGCCCAT TGGTCAGTTT GGTACCAGGC TACATGGTGG CAAGGATTCT
    GCTAGTCCAC GATACATCTT TACAATGCTC AGCTCTTTGG CTCGATTGTT ATTTCCACCA
    AAAGATGATC ACACGTTGAA GTTTTTATAT GATGACAACC AGCGTGTTGA GCCTGAATGG
    TACATTCCTA TTATTCCCAT GGTGCTGATA AATGGTGCTG AAGGAATCGG TACTGGGTGG
    TCCTGCAAAA TCCCCAACTT TGATGTGCGT GAAATTGTAA ATAACATCAG GCGTTTGATG
    GATGGAGAAG AACCATTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACTATTGAA
    GAACTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTAG CTATTCTTAA TTCTACAACC
    ATTGAAATCT CAGAGCTTCC CATCAGAACA TGGACCCAGA CATACAAAGA ACAAGTTCTA
    GAACCCATGT TGAATGGCAC CGAGAAGACA CCTCCTCTCA TAACAGACTA TAGGGAATAC
    CATACAGATA CCACTGTGAA ATTTGTTGTG AAGATGACTG AAGAAAAACT GGCAGAGGCA
    GAGAGAGTTG GACTACACAA AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCTATG
    GTGCTTTTTG ACCACGTAGG CTGTTTAAAG AAATATGACA CGGTGTTGGA TATTCTAAGA
    GACTTTTTTG AACTCAGACT TAAATATTAT GGATTAAGAA AAGAATGGCT CCTAGGAATG
    CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT
    GGCAAAATAA TCATTGAAAA TAAGCCTAAG AAAGAATTAA TTAAAGTTCT GATTCAGAGG
    GGATATGATT CGGATCCTGT GAAGGCCTGG AAAGAAGCCC AGCAAAAGGT TCCAGATGAA
    GAAGAAAATG AAGAGAGTGA CAACGAAAAG GAAACTGAAA AGAGTGACTC CGTAACAGAT
    TCTGGACCAA CCTTCAACTA TCTTCTTGAT ATGCCCCTTT GGTATTTAAC CAAGGAAAAG
    AAAGATGAAC TCTGCAGGCT AAGAAATGAA AAAGAACAAG AGCTGGACAC ATTAAAAAGA
    AAGAGTCCAT CAGATTTGTG GAAAGAAGAC TTGGCTACAT TTATTGAAGA ATTGGAGGCT
    GTTGAAGCCA AGGAAAAACA AGATGAACAA GTCGGACTTC CTGGGAAAGG GGGGAAGGCC
    AAGGGGAAAA AAACACAAAT GGCTGAAGTT TTGCCTTCTC CACGTGGTCA AAGAGTCATT
    CCACGAATAA CCATAGAAAT GAAAGCAGAG GCAGAAAAGA AAAATAAAAA GAAAATTAAG
    AATGAAAATA CTGAAGGAAG CCCTCAAGAA GATGGTGTGG AACTAGAAGG CCTAAAACAA
    AGATTAGAAA AGAAACAGAA AAGAGAACCA GGTACAAAGA CAAAGAAACA AACTACATTG
    CCATTTAAGC CAATCAAAAA AGGAAAGAAG AGAAATCCCT GGTCTGATTC AGAATCAGAT
    AGGAGCAGTG ACGAAAGTAA TTTTGATGTC CCTCCACGAG AAACAGAGCC ACGGAGAGCA
    GCAACAAAAA CCAAATTCAC AATGGATTTG GATTCAGATG AAGATTTCTC AGATTTTGAT
    GAAAAAACTG ATGATGAAGA TTTTGTCCCA TCAGATGCTA GTCCACCTAA GACCAAAACT
    TCCCCAAAAC TTAGTAACAA AGAACTGAAA CCACAGAAAA GTGTCGTGTC AGACCTTGAA
    GCTAATGATG CTAAGGACAG TGTACCACTG TCTTCAAGCC CTCCTGCTAC ACATTTCCCA
    GATGAAACTG AAATTACAAA CCCAGTTCCT AAAAAGAATG TGACAGTGAA GAAGACAGCA
    GCAAAAAGTC AGTCTTCCAC CTCCACTACC GGTGCCAAAA AAAGGGCTGC CCCAAAAGGA
    ACTAAAAGGG ATCCAGCTTT GAATTCTGGT GTCTCTCAGA AGCCTGATCC TGCCAAAACC
    AAGAATCGCC GCAAAAGGAA GCCATCCACT TCTGATGATT CTGACTCTAA TTTTGAGAAA
    ATTGTTTCGA AAGCAGTCAC AAGCAAGAAA TCCAAGGGGG AGAGTGATGA CTTCCATATG
    GACTTTGACT CAGCTGTGGC TCCTCGGGCA AAATCTGTAC GGGCAAAGAA ACCTATAAAG
    TACCTGGAAG AGTCAGATGA AGATGATCTG TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016787334.1
    CDS155..4750
    Translation

    Target ORF information:

    RefSeq Version XM_016931845.2
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes DNA topoisomerase II alpha (TOP2A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016931845.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAAGTGT CACCATTGCA GCCTGTAAAT GAAAATATGC AAGTCAACAA AATAAAGAAA 
    AATGAAGATG CTAAGAAAAG ACTGTCTGTT GAAAGAATCT ATCAAAAGAA AACACAATTG
    GAACATATTT TGCTCCGCCC AGACACCTAC ATTGGTTCTG TGGAATTAGT GACCCAGCAA
    ATGTGGGTTT ACGATGAAGA TGTTGGCATT AACTATAGGG AAGTCACTTT TGTTCCTGGT
    TTGTACAAAA TCTTTGATGA GATTCTAGTT AATGCTGCGG ACAACAAACA AAGGGACCCA
    AAAATGTCTT GTATTAGAGT CACAATTGAT CCGGAAAACA ATTTAATTAG TATATGGAAT
    AATGGAAAAG GTATTCCTGT TGTTGAACAC AAAGTTGAAA AGATGTATGT CCCAGCTCTC
    ATATTTGGAC AGCTCCTAAC TTCTAGTAAC TATGATGATG ATGAAAAGAA AGTGACAGGT
    GGTCGAAATG GCTATGGAGC CAAATTGTGT AACATATTCA GTACCAAATT TACTGTGGAA
    ACAGCCAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA TATGGGAAGA
    GCTGGTGAGA TGGAACTCAA GCCCTTCAAT GGAGAAGATT ATACATGTAT CACCTTTCAG
    CCTGATTTGT CTAAGTTTAA AATGCAAAGC CTGGACAAAG ATATTGTTGC ACTAATGGTC
    AGAAGAGCAT ATGATATTGC TGGATCCACC AAAGATGTCA AAGTCTTTCT TAATGGAAAT
    AAACTGCCAG TAAAAGGATT TCGTAGTTAT GTGGACATGT ATTTGAAGGA CAAGTTGGAT
    GAAACTGGTA ACTCCTTGAA AGTAATACAT GAACAAGTAA ACCACAGGTG GGAAGTGTGT
    TTAACTATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTTG TCAACAGCAT TGCTACATCC
    AAGGGTGGCA GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT
    GTGAAGAAGA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTGAA AAATCACATG
    TGGATTTTTG TAAATGCCTT AATTGAAAAC CCAACCTTTG ACTCTCAGAC AAAAGAAAAC
    ATGACTTTAC AACCCAAGAG CTTTGGATCA ACCTGCCAAT TGAGTGAAAA ATTTATCAAA
    GCTGCCATTG GCTGTGGTAT TGTAGAAAGC ATACTAAACT GGGTGAAGTT TAAGGCCCAA
    GTCCAGTTAA ACAAGAAGTG TTCAGCTGTA AAACATAATA GAATCAAGGG AATTCCCAAA
    CTCGATGATG CCAATGATGC AGGGGGCCGA AACTCCACTG AGTGTACGCT TATCCTGACT
    GAGGGAGATT CAGCCAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAA
    TATGGGGTTT TCCCTCTTAG AGGAAAAATA CTCAATGTTC GAGAAGCTTC TCATAAGCAG
    ATCATGGAAA ATGCTGAGAT TAACAATATC ATCAAGATTG TGGGTCTTCA GTACAAGAAA
    AACTATGAAG ATGAAGATTC ATTGAAGACT CTTCGTTATG GGAAGATAAT GATTATGACA
    GATCAGGACC AAGATGGTTC CCACATCAAA GGCTTGCTGA TTAATTTTAT CCATCACAAC
    TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCCAT TGTAAAGGTG
    TCTAAAAACA AGCAAGAAAT GGCATTTTAC AGCCTTCCTG AATTTGAAGA ATGGAAGAGT
    TCTACTCCAA ATCATAAAAA ATGGAAAGTC AAATATTACA AAGGTTTGGG CACCAGCACA
    TCAAAGGAAG CTAAAGAATA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA GTTCAAATAT
    TCTGGTCCTG AAGATGATGC TGCTATCAGC CTGGCCTTTA GCAAAAAACA GATAGATGAT
    CGAAAGGAAT GGTTAACTAA TTTCATGGAG GATAGAAGAC AACGAAAGTT ACTTGGGCTT
    CCTGAGGATT ACTTGTATGG ACAAACTACC ACATATCTGA CATATAATGA CTTCATCAAC
    AAGGAACTTA TCTTGTTCTC AAATTCTGAT AACGAGAGAT CTATCCCTTC TATGGTGGAT
    GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACTTGCT TCAAACGGAA TGACAAGCGA
    GAGGTAAAGG TTGCCCAATT AGCTGGATCA GTGGCTGAAA TGTCTTCTTA TCATCATGGT
    GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ATTTTGTGGG TAGCAATAAT
    CTAAACCTCT TGCAGCCCAT TGGTCAGTTT GGTACCAGGC TACATGGTGG CAAGGATTCT
    GCTAGTCCAC GATACATCTT TACAATGCTC AGCTCTTTGG CTCGATTGTT ATTTCCACCA
    AAAGATGATC ACACGTTGAA GTTTTTATAT GATGACAACC AGCGTGTTGA GCCTGAATGG
    TACATTCCTA TTATTCCCAT GGTGCTGATA AATGGTGCTG AAGGAATCGG TACTGGGTGG
    TCCTGCAAAA TCCCCAACTT TGATGTGCGT GAAATTGTAA ATAACATCAG GCGTTTGATG
    GATGGAGAAG AACCATTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACTATTGAA
    GAACTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTAG CTATTCTTAA TTCTACAACC
    ATTGAAATCT CAGAGCTTCC CATCAGAACA TGGACCCAGA CATACAAAGA ACAAGTTCTA
    GAACCCATGT TGAATGGCAC CGAGAAGACA CCTCCTCTCA TAACAGACTA TAGGGAATAC
    CATACAGATA CCACTGTGAA ATTTGTTGTG AAGATGACTG AAGAAAAACT GGCAGAGGCA
    GAGAGAGTTG GACTACACAA AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCTATG
    GTGCTTTTTG ACCACGTAGG CTGTTTAAAG AAATATGACA CGGTGTTGGA TATTCTAAGA
    GACTTTTTTG AACTCAGACT TAAATATTAT GGATTAAGAA AAGAATGGCT CCTAGGAATG
    CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT
    GGCAAAATAA TCATTGAAAA TAAGCCTAAG AAAGAATTAA TTAAAGTTCT GATTCAGAGG
    GGATATGATT CGGATCCTGT GAAGGCCTGG AAAGAAGCCC AGCAAAAGGT TCCAGATGAA
    GAAGAAAATG AAGAGAGTGA CAACGAAAAG GAAACTGAAA AGAGTGACTC CGTAACAGAT
    TCTGGACCAA CCTTCAACTA TCTTCTTGAT ATGCCCCTTT GGTATTTAAC CAAGGAAAAG
    AAAGATGAAC TCTGCAGGCT AAGAAATGAA AAAGAACAAG AGCTGGACAC ATTAAAAAGA
    AAGAGTCCAT CAGATTTGTG GAAAGAAGAC TTGGCTACAT TTATTGAAGA ATTGGAGGCT
    GTTGAAGCCA AGGAAAAACA AGATGAACAA GTCGGACTTC CTGGGAAAGG GGGGAAGGCC
    AAGGGGAAAA AAACACAAAT GGCTGAAGTT TTGCCTTCTC CACGTGGTCA AAGAGTCATT
    CCACGAATAA CCATAGAAAT GAAAGCAGAG GCAGAAAAGA AAAATAAAAA GAAAATTAAG
    AATGAAAATA CTGAAGGAAG CCCTCAAGAA GATGGTGTGG AACTAGAAGG CCTAAAACAA
    AGATTAGAAA AGAAACAGAA AAGAGAACCA GGTACAAAGA CAAAGAAACA AACTACATTG
    CCATTTAAGC CAATCAAAAA AGGAAAGAAG AGAAATCCCT GGTCTGATTC AGAATCAGAT
    AGGAGCAGTG ACGAAAGTAA TTTTGATGTC CCTCCACGAG AAACAGAGCC ACGGAGAGCA
    GCAACAAAAA CCAAATTCAC AATGGATTTG GATTCAGATG AAGATTTCTC AGATTTTGAT
    GAAAAAACTG ATGATGAAGA TTTTGTCCCA TCAGATGCTA GTCCACCTAA GACCAAAACT
    TCCCCAAAAC TTAGTAACAA AGAACTGAAA CCACAGAAAA GTGTCGTGTC AGACCTTGAA
    GCTAATGATG CTAAGGACAG TGTACCACTG TCTTCAAGCC CTCCTGCTAC ACATTTCCCA
    GATGAAACTG AAATTACAAA CCCAGTTCCT AAAAAGAATG TGACAGTGAA GAAGACAGCA
    GCAAAAAGTC AGTCTTCCAC CTCCACTACC GGTGCCAAAA AAAGGGCTGC CCCAAAAGGA
    ACTAAAAGGG ATCCAGCTTT GAATTCTGGT GTCTCTCAGA AGCCTGATCC TGCCAAAACC
    AAGAATCGCC GCAAAAGGAA GCCATCCACT TCTGATGATT CTGACTCTAA TTTTGAGAAA
    ATTGTTTCGA AAGCAGTCAC AAGCAAGAAA TCCAAGGGGG AGAGTGATGA CTTCCATATG
    GACTTTGACT CAGCTGTGGC TCCTCGGGCA AAATCTGTAC GGGCAAAGAA ACCTATAAAG
    TACCTGGAAG AGTCAGATGA AGATGATCTG TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.