NCKAP1 cDNA ORF clone, Gallus gallus(chicken)

The following NCKAP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCKAP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa38281 XM_015289348.2
Latest version!
Gallus gallus NCK associated protein 1 (NCKAP1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa38282 XM_015289349.2
Latest version!
Gallus gallus NCK associated protein 1 (NCKAP1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa38283 XM_015289350.2
Latest version!
Gallus gallus NCK associated protein 1 (NCKAP1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa38284 XM_015289351.2
Latest version!
Gallus gallus NCK associated protein 1 (NCKAP1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa10694 XM_004942427.3
Latest version!
Gallus gallus NCK associated protein 1 (NCKAP1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OGa38281 XM_015289348.1 Gallus gallus NCK-associated protein 1 (NCKAP1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa38284 XM_015289351.1 Gallus gallus NCK-associated protein 1 (NCKAP1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa10694 XM_004942427.2 Gallus gallus NCK-associated protein 1 (NCKAP1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OGa38282 XM_015289349.1 Gallus gallus NCK-associated protein 1 (NCKAP1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa38283 XM_015289350.1 Gallus gallus NCK-associated protein 1 (NCKAP1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa38281
    Clone ID Related Accession (Same CDS sequence) XM_015289348.2 , XM_015289348.1
    Accession Version XM_015289348.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3405bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product nck-associated protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289348.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCGCGGT CCGTGCTTCA GCCGAGCCAG CAGAAGCTGG CCGAGAAGCT GACCATCCTA 
    AACGACCGGG GCGTCGGCAT GCTCACCCGG CTCTACAACA TCAAGAAGCA AGGACAAGTT
    TGGAAGGCAT GTGGAGACCC CAAAGCCAAA CCATCCTATC TTATTGACAA AAACCTGGAA
    TCTGCAGTCA AATTCATAGT CAGGAAGTTT CCAGCGGTAG AAACACGCAA CAACAATCAA
    CAGCTTGCTC AGCTACAGAA AGAGAAGTCA GAGATTTTGA AGAACCTGGC GTTGTACTAC
    TTCACGTTTG TTGATGTTAT GGAATTTAAG GACCATGTTT GCGAGCTGCT GAACACTATT
    GATGTTTGCC AAGTCTTCTT TGATATTACT GTAAACTTTG ATTTGACGAA GAACTACCTG
    GACTTGATTA TAACCTACAC TACACTAATG ATACTGCTGT CTCGAATTGA AGAAAGGAAG
    GCTATCATTG GATTGTATAA CTATGCTCAT GAAATGACAC ATGGAGCGAG TGACAGAGAA
    TATCCACGCC TTGGCCAGAT GATTGTGGAT TATGAAAACC CTTTAAAGAA AATGATGGAG
    GAATTTGTAC CCCACAGTAA ATCCCTTTCG GATGCTCTGA TTTCACTCCA GATGGTCTAT
    CCTCGAAGAA ATCTCTCGGC TGACCAGTGG AGAAACGCAC AACTACTGAG CCTCATCAGT
    GCACCTAGTA CAATGCTTAA CCCTGCACAG TCTGACACGA TGCCATGTGA ATACCTCTCT
    CTGGATGCAA TGGAAAAATG GATTATTTTT GGATTTATTT TGTGCCATGG AGTCCTAAAT
    AGTGATGCGA CAGCTTTGAA CCTTTGGAAA CTTGCACTTC AAAGCAGCTC CTGCTTGGCT
    CTCTTTAGAG ATGAAGTATT CCATATTCAC AAAGCTGCAG AAGACTTATT TGTAAACATA
    CGAGGCTACA ACAAGCGTAT TAATGACATC AGAGAGTGTA AAGAAGCTGC TGTTTCACAT
    GCCGGTTCGA TGCACAGAGA GAGGCGCAAG TTTTTACGTT CTGCACTCAA AGAACTGGCG
    ACAGTCCTGT CTGATCAGCC AGGCCTGCTG GGCCCCAAGG CACTTTTTGT ATTTATGGCA
    TTATCCTTTG CTCGTGATGA AATCATTTGG CTTCTTCGTC ATGCAGATAA CATGCCAAAG
    AAGAGTGCAG ATGACTTCAT AGATAAGCAC ATAGCTGAGC TAATATTCTA CATGGAGGAG
    CTCAGAGCAC ATGTGCGAAA ATACGGACCT GTGATGCAGA GATACTACGT ACAGTATCTG
    TCTGGCTTTG ATGCAGTGGT CCTAAATGAA CTTGTTCAGA ATCTTTCGGT GTGCCCAGAG
    GATGAATCAA TCATCATGTC CTCTTTTGTA AACACTATGA CTTCGCTAAG TGTGAAGCAG
    GTTGAGGATG GAGAGGTATT TGACTTCAGA GGAATGAGAT TAGATTGGTT TAGATTGCAG
    GCCTACACCA GTGTCTCTAA AGCTTCTCTT GGACTGGCAG ACCACAGGGA GCTAGGCAAA
    ATGATGAACA CAATCATCTT CCACACAAAA ATGGTAGATT CTTTGGTGGA GATGTTGGTT
    GAAACCTCAG ATCTTTCTAT ATTTTGTTTT TATAGTCGTG CTTTTGAGAA GATGTTTCAG
    CAGTGTTTGG AGCTTCCCTC TCAATCAAGA TATTCAATTG CATTCCCACT GCTTTGCACT
    CATTTTATGA GTTGCACCCA CGAACTGTGC CCAGAAGAGC GACATCACAT CGGAGATCGT
    AGTCTTTCCT TATGTAACAT GTTTCTGGAT GAAATGGCTA AGCAAGCTCG AAATCTTATC
    ACAGACATTT GCACAGAACA ATGTACGCTG AGTGATCAGT TGCTGCCAAA GCATTGTGCC
    AAAACCATCA GCCAAGCAGT GAACAAAAAA TCCAAAAAAC AGACAGGAAA GAAAGGAGAA
    CCTGAAAGGG AGAAACCAGG AGTAGAAAGC ATGAGAAAAA ACAGATTGGT TGTGACAAAC
    CTGGACAAAC TGCACACTGC ACTTTCGGAG CTCTGCTTCT CAATCAATTA TGTACCAAAC
    ATGGTGGTTT GGGAGCACAC ATTTACCCCA AGAGAATATT TAACGTCTCA CCTGGAAATC
    CGCTTTACCA AGTCCATTGT TGGAATGACG ATGTATAATC AAGCAACACA GGAGATTGCA
    AAGCCTTCAG AGCTGTTAAC CAGTGTAAGA GCCTATATGA CGGTCCTCCA GTCAATAGAA
    AACTATGTAC AGATTGACAT TACACGAGTA TTTAACAATG TTCTGCTTCA GCAAACTCAG
    CATTTAGATA GTCATGGTGA GCCAACCATC ACCAGTCTGT ACACAAATTG GTATTTGGAA
    ACATTGCTAA GGCAAGTCAG CAATGGTCAC ATAGCCTATT TTCCAGCTAT GAAGGCATTT
    GTGAATTTGC CAACAGAAAA TGAATTAACA TTTAATGCTG AGGAGTACTC TGACATATCA
    GAAATGAGAG CCCTTTCTGA ACTCCTGGGA CCATATGGCA TGAAGTTCTT AAGCGAAAGT
    CTGATGTGGC ACATTTCTTC GCAGGTTGCC GAGCTTAAGA AACTCGTGGT GGAGAATGTT
    GAAGTTCTAA CACAAATGAG GACCAGCTTC GATAAGCCAG ACCAGATGGC AGCACTCTTC
    AAAAGATTAT CATCTGTTGA CAGTGTTTTG AAGAGAATGA CGATTATTGG TGTCATCTTG
    TCTTTTCGGT CGTTGGCACA GGAAGCACTT AGAGATGTCT TATCCTACCA CATTCCTTTC
    CTTGTAAGTT CCATTGAGGA CTTCAAAGAT CACATTCCAA GAGAGACTGA CATGAAGGTG
    GCAATGAATG TATATGAACT GTCATCAGCT GCTGGATTGC CTTGCGAGAT TGATCCTGCC
    TTGGTTGTAG CACTGTCCTC CCAAAAATCA GAAAACATCA GTCCAGAAGA AGAATACAAA
    ATTGCTTGCC TTCTCATGGT CTTCGTGGCA GTCTCCTTGC CAACTTTGGC TAGTAACGTG
    ATGTCTCAGT ATAGCCCTGC CATTGAAGGG CACTGCAACA ACATTCATTG CTTGGCAAAA
    GCTATCAACC AGATTGCTGC AGCTTTATTT ACCATCCACA AGGGAAGCAT CGAAGACCGT
    CTAAAAGAAT TTTTGGCTCT TGCATCATCT AGTCTACTGA AGATCGGCCA GGAGACGGAC
    AAAACTACTA CAAGAAACAG AGAGTCTGTT TACCTACTAC TGGACATGAT TGTGCAGGAG
    TCCCCGTTCC TGACGATGGA CCTCCTGGAG TCGTGCTTCC CCTACGTCTT GCTGAGAAAC
    GCGTACCACG CTGTCTACAA ACAAAGCGTC ACCTCCTCTG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015144834.1
    CDS1..3405
    Translation

    Target ORF information:

    RefSeq Version XM_015289348.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus NCK associated protein 1 (NCKAP1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289348.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCGCGGT CCGTGCTTCA GCCGAGCCAG CAGAAGCTGG CCGAGAAGCT GACCATCCTA 
    AACGACCGGG GCGTCGGCAT GCTCACCCGG CTCTACAACA TCAAGAAGCA AGGACAAGTT
    TGGAAGGCAT GTGGAGACCC CAAAGCCAAA CCATCCTATC TTATTGACAA AAACCTGGAA
    TCTGCAGTCA AATTCATAGT CAGGAAGTTT CCAGCGGTAG AAACACGCAA CAACAATCAA
    CAGCTTGCTC AGCTACAGAA AGAGAAGTCA GAGATTTTGA AGAACCTGGC GTTGTACTAC
    TTCACGTTTG TTGATGTTAT GGAATTTAAG GACCATGTTT GCGAGCTGCT GAACACTATT
    GATGTTTGCC AAGTCTTCTT TGATATTACT GTAAACTTTG ATTTGACGAA GAACTACCTG
    GACTTGATTA TAACCTACAC TACACTAATG ATACTGCTGT CTCGAATTGA AGAAAGGAAG
    GCTATCATTG GATTGTATAA CTATGCTCAT GAAATGACAC ATGGAGCGAG TGACAGAGAA
    TATCCACGCC TTGGCCAGAT GATTGTGGAT TATGAAAACC CTTTAAAGAA AATGATGGAG
    GAATTTGTAC CCCACAGTAA ATCCCTTTCG GATGCTCTGA TTTCACTCCA GATGGTCTAT
    CCTCGAAGAA ATCTCTCGGC TGACCAGTGG AGAAACGCAC AACTACTGAG CCTCATCAGT
    GCACCTAGTA CAATGCTTAA CCCTGCACAG TCTGACACGA TGCCATGTGA ATACCTCTCT
    CTGGATGCAA TGGAAAAATG GATTATTTTT GGATTTATTT TGTGCCATGG AGTCCTAAAT
    AGTGATGCGA CAGCTTTGAA CCTTTGGAAA CTTGCACTTC AAAGCAGCTC CTGCTTGGCT
    CTCTTTAGAG ATGAAGTATT CCATATTCAC AAAGCTGCAG AAGACTTATT TGTAAACATA
    CGAGGCTACA ACAAGCGTAT TAATGACATC AGAGAGTGTA AAGAAGCTGC TGTTTCACAT
    GCCGGTTCGA TGCACAGAGA GAGGCGCAAG TTTTTACGTT CTGCACTCAA AGAACTGGCG
    ACAGTCCTGT CTGATCAGCC AGGCCTGCTG GGCCCCAAGG CACTTTTTGT ATTTATGGCA
    TTATCCTTTG CTCGTGATGA AATCATTTGG CTTCTTCGTC ATGCAGATAA CATGCCAAAG
    AAGAGTGCAG ATGACTTCAT AGATAAGCAC ATAGCTGAGC TAATATTCTA CATGGAGGAG
    CTCAGAGCAC ATGTGCGAAA ATACGGACCT GTGATGCAGA GATACTACGT ACAGTATCTG
    TCTGGCTTTG ATGCAGTGGT CCTAAATGAA CTTGTTCAGA ATCTTTCGGT GTGCCCAGAG
    GATGAATCAA TCATCATGTC CTCTTTTGTA AACACTATGA CTTCGCTAAG TGTGAAGCAG
    GTTGAGGATG GAGAGGTATT TGACTTCAGA GGAATGAGAT TAGATTGGTT TAGATTGCAG
    GCCTACACCA GTGTCTCTAA AGCTTCTCTT GGACTGGCAG ACCACAGGGA GCTAGGCAAA
    ATGATGAACA CAATCATCTT CCACACAAAA ATGGTAGATT CTTTGGTGGA GATGTTGGTT
    GAAACCTCAG ATCTTTCTAT ATTTTGTTTT TATAGTCGTG CTTTTGAGAA GATGTTTCAG
    CAGTGTTTGG AGCTTCCCTC TCAATCAAGA TATTCAATTG CATTCCCACT GCTTTGCACT
    CATTTTATGA GTTGCACCCA CGAACTGTGC CCAGAAGAGC GACATCACAT CGGAGATCGT
    AGTCTTTCCT TATGTAACAT GTTTCTGGAT GAAATGGCTA AGCAAGCTCG AAATCTTATC
    ACAGACATTT GCACAGAACA ATGTACGCTG AGTGATCAGT TGCTGCCAAA GCATTGTGCC
    AAAACCATCA GCCAAGCAGT GAACAAAAAA TCCAAAAAAC AGACAGGAAA GAAAGGAGAA
    CCTGAAAGGG AGAAACCAGG AGTAGAAAGC ATGAGAAAAA ACAGATTGGT TGTGACAAAC
    CTGGACAAAC TGCACACTGC ACTTTCGGAG CTCTGCTTCT CAATCAATTA TGTACCAAAC
    ATGGTGGTTT GGGAGCACAC ATTTACCCCA AGAGAATATT TAACGTCTCA CCTGGAAATC
    CGCTTTACCA AGTCCATTGT TGGAATGACG ATGTATAATC AAGCAACACA GGAGATTGCA
    AAGCCTTCAG AGCTGTTAAC CAGTGTAAGA GCCTATATGA CGGTCCTCCA GTCAATAGAA
    AACTATGTAC AGATTGACAT TACACGAGTA TTTAACAATG TTCTGCTTCA GCAAACTCAG
    CATTTAGATA GTCATGGTGA GCCAACCATC ACCAGTCTGT ACACAAATTG GTATTTGGAA
    ACATTGCTAA GGCAAGTCAG CAATGGTCAC ATAGCCTATT TTCCAGCTAT GAAGGCATTT
    GTGAATTTGC CAACAGAAAA TGAATTAACA TTTAATGCTG AGGAGTACTC TGACATATCA
    GAAATGAGAG CCCTTTCTGA ACTCCTGGGA CCATATGGCA TGAAGTTCTT AAGCGAAAGT
    CTGATGTGGC ACATTTCTTC GCAGGTTGCC GAGCTTAAGA AACTCGTGGT GGAGAATGTT
    GAAGTTCTAA CACAAATGAG GACCAGCTTC GATAAGCCAG ACCAGATGGC AGCACTCTTC
    AAAAGATTAT CATCTGTTGA CAGTGTTTTG AAGAGAATGA CGATTATTGG TGTCATCTTG
    TCTTTTCGGT CGTTGGCACA GGAAGCACTT AGAGATGTCT TATCCTACCA CATTCCTTTC
    CTTGTAAGTT CCATTGAGGA CTTCAAAGAT CACATTCCAA GAGAGACTGA CATGAAGGTG
    GCAATGAATG TATATGAACT GTCATCAGCT GCTGGATTGC CTTGCGAGAT TGATCCTGCC
    TTGGTTGTAG CACTGTCCTC CCAAAAATCA GAAAACATCA GTCCAGAAGA AGAATACAAA
    ATTGCTTGCC TTCTCATGGT CTTCGTGGCA GTCTCCTTGC CAACTTTGGC TAGTAACGTG
    ATGTCTCAGT ATAGCCCTGC CATTGAAGGG CACTGCAACA ACATTCATTG CTTGGCAAAA
    GCTATCAACC AGATTGCTGC AGCTTTATTT ACCATCCACA AGGGAAGCAT CGAAGACCGT
    CTAAAAGAAT TTTTGGCTCT TGCATCATCT AGTCTACTGA AGATCGGCCA GGAGACGGAC
    AAAACTACTA CAAGAAACAG AGAGTCTGTT TACCTACTAC TGGACATGAT TGTGCAGGAG
    TCCCCGTTCC TGACGATGGA CCTCCTGGAG TCGTGCTTCC CCTACGTCTT GCTGAGAAAC
    GCGTACCACG CTGTCTACAA ACAAAGCGTC ACCTCCTCTG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa38281
    Clone ID Related Accession (Same CDS sequence) XM_015289348.2 , XM_015289348.1
    Accession Version XM_015289348.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3405bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product nck-associated protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455901.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCGCGGT CCGTGCTTCA GCCGAGCCAG CAGAAGCTGG CCGAGAAGCT GACCATCCTA 
    AACGACCGGG GCGTCGGCAT GCTCACCCGG CTCTACAACA TCAAGAAGCA AGGACAAGTT
    TGGAAGGCAT GTGGAGACCC CAAAGCCAAA CCATCCTATC TTATTGACAA AAACCTGGAA
    TCTGCAGTCA AATTCATAGT CAGGAAGTTT CCAGCGGTAG AAACACGCAA CAACAATCAA
    CAGCTTGCTC AGCTACAGAA AGAGAAGTCA GAGATTTTGA AGAACCTGGC GTTGTACTAC
    TTCACGTTTG TTGATGTTAT GGAATTTAAG GACCATGTTT GCGAGCTGCT GAACACTATT
    GATGTTTGCC AAGTCTTCTT TGATATTACT GTAAACTTTG ATTTGACGAA GAACTACCTG
    GACTTGATTA TAACCTACAC TACACTAATG ATACTGCTGT CTCGAATTGA AGAAAGGAAG
    GCTATCATTG GATTGTATAA CTATGCTCAT GAAATGACAC ATGGAGCGAG TGACAGAGAA
    TATCCACGCC TTGGCCAGAT GATTGTGGAT TATGAAAACC CTTTAAAGAA AATGATGGAG
    GAATTTGTAC CCCACAGTAA ATCCCTTTCG GATGCTCTGA TTTCACTCCA GATGGTCTAT
    CCTCGAAGAA ATCTCTCGGC TGACCAGTGG AGAAACGCAC AACTACTGAG CCTCATCAGT
    GCACCTAGTA CAATGCTTAA CCCTGCACAG TCTGACACGA TGCCATGTGA ATACCTCTCT
    CTGGATGCAA TGGAAAAATG GATTATTTTT GGATTTATTT TGTGCCATGG AGTCCTAAAT
    AGTGATGCGA CAGCTTTGAA CCTTTGGAAA CTTGCACTTC AAAGCAGCTC CTGCTTGGCT
    CTCTTTAGAG ATGAAGTATT CCATATTCAC AAAGCTGCAG AAGACTTATT TGTAAACATA
    CGAGGCTACA ACAAGCGTAT TAATGACATC AGAGAGTGTA AAGAAGCTGC TGTTTCACAT
    GCCGGTTCGA TGCACAGAGA GAGGCGCAAG TTTTTACGTT CTGCACTCAA AGAACTGGCG
    ACAGTCCTGT CTGATCAGCC AGGCCTGCTG GGCCCCAAGG CACTTTTTGT ATTTATGGCA
    TTATCCTTTG CTCGTGATGA AATCATTTGG CTTCTTCGTC ATGCAGATAA CATGCCAAAG
    AAGAGTGCAG ATGACTTCAT AGATAAGCAC ATAGCTGAGC TAATATTCTA CATGGAGGAG
    CTCAGAGCAC ATGTGCGAAA ATACGGACCT GTGATGCAGA GATACTACGT ACAGTATCTG
    TCTGGCTTTG ATGCAGTGGT CCTAAATGAA CTTGTTCAGA ATCTTTCGGT GTGCCCAGAG
    GATGAATCAA TCATCATGTC CTCTTTTGTA AACACTATGA CTTCGCTAAG TGTGAAGCAG
    GTTGAGGATG GAGAGGTATT TGACTTCAGA GGAATGAGAT TAGATTGGTT TAGATTGCAG
    GCCTACACCA GTGTCTCTAA AGCTTCTCTT GGACTGGCAG ACCACAGGGA GCTAGGCAAA
    ATGATGAACA CAATCATCTT CCACACAAAA ATGGTAGATT CTTTGGTGGA GATGTTGGTT
    GAAACCTCAG ATCTTTCTAT ATTTTGTTTT TATAGTCGTG CTTTTGAGAA GATGTTTCAG
    CAGTGTTTGG AGCTTCCCTC TCAATCAAGA TATTCAATTG CATTCCCACT GCTTTGCACT
    CATTTTATGA GTTGCACCCA CGAACTGTGC CCAGAAGAGC GACATCACAT CGGAGATCGT
    AGTCTTTCCT TATGTAACAT GTTTCTGGAT GAAATGGCTA AGCAAGCTCG AAATCTTATC
    ACAGACATTT GCACAGAACA ATGTACGCTG AGTGATCAGT TGCTGCCAAA GCATTGTGCC
    AAAACCATCA GCCAAGCAGT GAACAAAAAA TCCAAAAAAC AGACAGGAAA GAAAGGAGAA
    CCTGAAAGGG AGAAACCAGG AGTAGAAAGC ATGAGAAAAA ACAGATTGGT TGTGACAAAC
    CTGGACAAAC TGCACACTGC ACTTTCGGAG CTCTGCTTCT CAATCAATTA TGTACCAAAC
    ATGGTGGTTT GGGAGCACAC ATTTACCCCA AGAGAATATT TAACGTCTCA CCTGGAAATC
    CGCTTTACCA AGTCCATTGT TGGAATGACG ATGTATAATC AAGCAACACA GGAGATTGCA
    AAGCCTTCAG AGCTGTTAAC CAGTGTAAGA GCCTATATGA CGGTCCTCCA GTCAATAGAA
    AACTATGTAC AGATTGACAT TACACGAGTA TTTAACAATG TTCTGCTTCA GCAAACTCAG
    CATTTAGATA GTCATGGTGA GCCAACCATC ACCAGTCTGT ACACAAATTG GTATTTGGAA
    ACATTGCTAA GGCAAGTCAG CAATGGTCAC ATAGCCTATT TTCCAGCTAT GAAGGCATTT
    GTGAATTTGC CAACAGAAAA TGAATTAACA TTTAATGCTG AGGAGTACTC TGACATATCA
    GAAATGAGAG CCCTTTCTGA ACTCCTGGGA CCATATGGCA TGAAGTTCTT AAGCGAAAGT
    CTGATGTGGC ACATTTCTTC GCAGGTTGCC GAGCTTAAGA AACTCGTGGT GGAGAATGTT
    GAAGTTCTAA CACAAATGAG GACCAGCTTC GATAAGCCAG ACCAGATGGC AGCACTCTTC
    AAAAGATTAT CATCTGTTGA CAGTGTTTTG AAGAGAATGA CGATTATTGG TGTCATCTTG
    TCTTTTCGGT CGTTGGCACA GGAAGCACTT AGAGATGTCT TATCCTACCA CATTCCTTTC
    CTTGTAAGTT CCATTGAGGA CTTCAAAGAT CACATTCCAA GAGAGACTGA CATGAAGGTG
    GCAATGAATG TATATGAACT GTCATCAGCT GCTGGATTGC CTTGCGAGAT TGATCCTGCC
    TTGGTTGTAG CACTGTCCTC CCAAAAATCA GAAAACATCA GTCCAGAAGA AGAATACAAA
    ATTGCTTGCC TTCTCATGGT CTTCGTGGCA GTCTCCTTGC CAACTTTGGC TAGTAACGTG
    ATGTCTCAGT ATAGCCCTGC CATTGAAGGG CACTGCAACA ACATTCATTG CTTGGCAAAA
    GCTATCAACC AGATTGCTGC AGCTTTATTT ACCATCCACA AGGGAAGCAT CGAAGACCGT
    CTAAAAGAAT TTTTGGCTCT TGCATCATCT AGTCTACTGA AGATCGGCCA GGAGACGGAC
    AAAACTACTA CAAGAAACAG AGAGTCTGTT TACCTACTAC TGGACATGAT TGTGCAGGAG
    TCCCCGTTCC TGACGATGGA CCTCCTGGAG TCGTGCTTCC CCTACGTCTT GCTGAGAAAC
    GCGTACCACG CTGTCTACAA ACAAAGCGTC ACCTCCTCTG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015144834.1
    CDS52..3456
    Translation

    Target ORF information:

    RefSeq Version XM_015289348.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus NCK-associated protein 1 (NCKAP1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289348.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCGCGGT CCGTGCTTCA GCCGAGCCAG CAGAAGCTGG CCGAGAAGCT GACCATCCTA 
    AACGACCGGG GCGTCGGCAT GCTCACCCGG CTCTACAACA TCAAGAAGCA AGGACAAGTT
    TGGAAGGCAT GTGGAGACCC CAAAGCCAAA CCATCCTATC TTATTGACAA AAACCTGGAA
    TCTGCAGTCA AATTCATAGT CAGGAAGTTT CCAGCGGTAG AAACACGCAA CAACAATCAA
    CAGCTTGCTC AGCTACAGAA AGAGAAGTCA GAGATTTTGA AGAACCTGGC GTTGTACTAC
    TTCACGTTTG TTGATGTTAT GGAATTTAAG GACCATGTTT GCGAGCTGCT GAACACTATT
    GATGTTTGCC AAGTCTTCTT TGATATTACT GTAAACTTTG ATTTGACGAA GAACTACCTG
    GACTTGATTA TAACCTACAC TACACTAATG ATACTGCTGT CTCGAATTGA AGAAAGGAAG
    GCTATCATTG GATTGTATAA CTATGCTCAT GAAATGACAC ATGGAGCGAG TGACAGAGAA
    TATCCACGCC TTGGCCAGAT GATTGTGGAT TATGAAAACC CTTTAAAGAA AATGATGGAG
    GAATTTGTAC CCCACAGTAA ATCCCTTTCG GATGCTCTGA TTTCACTCCA GATGGTCTAT
    CCTCGAAGAA ATCTCTCGGC TGACCAGTGG AGAAACGCAC AACTACTGAG CCTCATCAGT
    GCACCTAGTA CAATGCTTAA CCCTGCACAG TCTGACACGA TGCCATGTGA ATACCTCTCT
    CTGGATGCAA TGGAAAAATG GATTATTTTT GGATTTATTT TGTGCCATGG AGTCCTAAAT
    AGTGATGCGA CAGCTTTGAA CCTTTGGAAA CTTGCACTTC AAAGCAGCTC CTGCTTGGCT
    CTCTTTAGAG ATGAAGTATT CCATATTCAC AAAGCTGCAG AAGACTTATT TGTAAACATA
    CGAGGCTACA ACAAGCGTAT TAATGACATC AGAGAGTGTA AAGAAGCTGC TGTTTCACAT
    GCCGGTTCGA TGCACAGAGA GAGGCGCAAG TTTTTACGTT CTGCACTCAA AGAACTGGCG
    ACAGTCCTGT CTGATCAGCC AGGCCTGCTG GGCCCCAAGG CACTTTTTGT ATTTATGGCA
    TTATCCTTTG CTCGTGATGA AATCATTTGG CTTCTTCGTC ATGCAGATAA CATGCCAAAG
    AAGAGTGCAG ATGACTTCAT AGATAAGCAC ATAGCTGAGC TAATATTCTA CATGGAGGAG
    CTCAGAGCAC ATGTGCGAAA ATACGGACCT GTGATGCAGA GATACTACGT ACAGTATCTG
    TCTGGCTTTG ATGCAGTGGT CCTAAATGAA CTTGTTCAGA ATCTTTCGGT GTGCCCAGAG
    GATGAATCAA TCATCATGTC CTCTTTTGTA AACACTATGA CTTCGCTAAG TGTGAAGCAG
    GTTGAGGATG GAGAGGTATT TGACTTCAGA GGAATGAGAT TAGATTGGTT TAGATTGCAG
    GCCTACACCA GTGTCTCTAA AGCTTCTCTT GGACTGGCAG ACCACAGGGA GCTAGGCAAA
    ATGATGAACA CAATCATCTT CCACACAAAA ATGGTAGATT CTTTGGTGGA GATGTTGGTT
    GAAACCTCAG ATCTTTCTAT ATTTTGTTTT TATAGTCGTG CTTTTGAGAA GATGTTTCAG
    CAGTGTTTGG AGCTTCCCTC TCAATCAAGA TATTCAATTG CATTCCCACT GCTTTGCACT
    CATTTTATGA GTTGCACCCA CGAACTGTGC CCAGAAGAGC GACATCACAT CGGAGATCGT
    AGTCTTTCCT TATGTAACAT GTTTCTGGAT GAAATGGCTA AGCAAGCTCG AAATCTTATC
    ACAGACATTT GCACAGAACA ATGTACGCTG AGTGATCAGT TGCTGCCAAA GCATTGTGCC
    AAAACCATCA GCCAAGCAGT GAACAAAAAA TCCAAAAAAC AGACAGGAAA GAAAGGAGAA
    CCTGAAAGGG AGAAACCAGG AGTAGAAAGC ATGAGAAAAA ACAGATTGGT TGTGACAAAC
    CTGGACAAAC TGCACACTGC ACTTTCGGAG CTCTGCTTCT CAATCAATTA TGTACCAAAC
    ATGGTGGTTT GGGAGCACAC ATTTACCCCA AGAGAATATT TAACGTCTCA CCTGGAAATC
    CGCTTTACCA AGTCCATTGT TGGAATGACG ATGTATAATC AAGCAACACA GGAGATTGCA
    AAGCCTTCAG AGCTGTTAAC CAGTGTAAGA GCCTATATGA CGGTCCTCCA GTCAATAGAA
    AACTATGTAC AGATTGACAT TACACGAGTA TTTAACAATG TTCTGCTTCA GCAAACTCAG
    CATTTAGATA GTCATGGTGA GCCAACCATC ACCAGTCTGT ACACAAATTG GTATTTGGAA
    ACATTGCTAA GGCAAGTCAG CAATGGTCAC ATAGCCTATT TTCCAGCTAT GAAGGCATTT
    GTGAATTTGC CAACAGAAAA TGAATTAACA TTTAATGCTG AGGAGTACTC TGACATATCA
    GAAATGAGAG CCCTTTCTGA ACTCCTGGGA CCATATGGCA TGAAGTTCTT AAGCGAAAGT
    CTGATGTGGC ACATTTCTTC GCAGGTTGCC GAGCTTAAGA AACTCGTGGT GGAGAATGTT
    GAAGTTCTAA CACAAATGAG GACCAGCTTC GATAAGCCAG ACCAGATGGC AGCACTCTTC
    AAAAGATTAT CATCTGTTGA CAGTGTTTTG AAGAGAATGA CGATTATTGG TGTCATCTTG
    TCTTTTCGGT CGTTGGCACA GGAAGCACTT AGAGATGTCT TATCCTACCA CATTCCTTTC
    CTTGTAAGTT CCATTGAGGA CTTCAAAGAT CACATTCCAA GAGAGACTGA CATGAAGGTG
    GCAATGAATG TATATGAACT GTCATCAGCT GCTGGATTGC CTTGCGAGAT TGATCCTGCC
    TTGGTTGTAG CACTGTCCTC CCAAAAATCA GAAAACATCA GTCCAGAAGA AGAATACAAA
    ATTGCTTGCC TTCTCATGGT CTTCGTGGCA GTCTCCTTGC CAACTTTGGC TAGTAACGTG
    ATGTCTCAGT ATAGCCCTGC CATTGAAGGG CACTGCAACA ACATTCATTG CTTGGCAAAA
    GCTATCAACC AGATTGCTGC AGCTTTATTT ACCATCCACA AGGGAAGCAT CGAAGACCGT
    CTAAAAGAAT TTTTGGCTCT TGCATCATCT AGTCTACTGA AGATCGGCCA GGAGACGGAC
    AAAACTACTA CAAGAAACAG AGAGTCTGTT TACCTACTAC TGGACATGAT TGTGCAGGAG
    TCCCCGTTCC TGACGATGGA CCTCCTGGAG TCGTGCTTCC CCTACGTCTT GCTGAGAAAC
    GCGTACCACG CTGTCTACAA ACAAAGCGTC ACCTCCTCTG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa38282
    Clone ID Related Accession (Same CDS sequence) XM_015289349.2 , XM_015289349.1
    Accession Version XM_015289349.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3399bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product nck-associated protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289349.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCGCGGT CCGTGCTTCA GCCGAGCCAG CAGAAGCTGG CCGAGAAGCT GACCATCCTA 
    AACGACCGGG GCGTCGGCAT GCTCACCCGG CTCTACAACA TCAAGAAGCA AGGACAAGTT
    TGGAAGGCAT GTGGAGACCC CAAAGCCAAA CCATCCTATC TTATTGACAA AAACCTGGAA
    TCTGCAGTCA AATTCATAGT CAGGAAGTTT CCAGCGGTAG AAACACGCAA CAACAATCTT
    GCTCAGCTAC AGAAAGAGAA GTCAGAGATT TTGAAGAACC TGGCGTTGTA CTACTTCACG
    TTTGTTGATG TTATGGAATT TAAGGACCAT GTTTGCGAGC TGCTGAACAC TATTGATGTT
    TGCCAAGTCT TCTTTGATAT TACTGTAAAC TTTGATTTGA CGAAGAACTA CCTGGACTTG
    ATTATAACCT ACACTACACT AATGATACTG CTGTCTCGAA TTGAAGAAAG GAAGGCTATC
    ATTGGATTGT ATAACTATGC TCATGAAATG ACACATGGAG CGAGTGACAG AGAATATCCA
    CGCCTTGGCC AGATGATTGT GGATTATGAA AACCCTTTAA AGAAAATGAT GGAGGAATTT
    GTACCCCACA GTAAATCCCT TTCGGATGCT CTGATTTCAC TCCAGATGGT CTATCCTCGA
    AGAAATCTCT CGGCTGACCA GTGGAGAAAC GCACAACTAC TGAGCCTCAT CAGTGCACCT
    AGTACAATGC TTAACCCTGC ACAGTCTGAC ACGATGCCAT GTGAATACCT CTCTCTGGAT
    GCAATGGAAA AATGGATTAT TTTTGGATTT ATTTTGTGCC ATGGAGTCCT AAATAGTGAT
    GCGACAGCTT TGAACCTTTG GAAACTTGCA CTTCAAAGCA GCTCCTGCTT GGCTCTCTTT
    AGAGATGAAG TATTCCATAT TCACAAAGCT GCAGAAGACT TATTTGTAAA CATACGAGGC
    TACAACAAGC GTATTAATGA CATCAGAGAG TGTAAAGAAG CTGCTGTTTC ACATGCCGGT
    TCGATGCACA GAGAGAGGCG CAAGTTTTTA CGTTCTGCAC TCAAAGAACT GGCGACAGTC
    CTGTCTGATC AGCCAGGCCT GCTGGGCCCC AAGGCACTTT TTGTATTTAT GGCATTATCC
    TTTGCTCGTG ATGAAATCAT TTGGCTTCTT CGTCATGCAG ATAACATGCC AAAGAAGAGT
    GCAGATGACT TCATAGATAA GCACATAGCT GAGCTAATAT TCTACATGGA GGAGCTCAGA
    GCACATGTGC GAAAATACGG ACCTGTGATG CAGAGATACT ACGTACAGTA TCTGTCTGGC
    TTTGATGCAG TGGTCCTAAA TGAACTTGTT CAGAATCTTT CGGTGTGCCC AGAGGATGAA
    TCAATCATCA TGTCCTCTTT TGTAAACACT ATGACTTCGC TAAGTGTGAA GCAGGTTGAG
    GATGGAGAGG TATTTGACTT CAGAGGAATG AGATTAGATT GGTTTAGATT GCAGGCCTAC
    ACCAGTGTCT CTAAAGCTTC TCTTGGACTG GCAGACCACA GGGAGCTAGG CAAAATGATG
    AACACAATCA TCTTCCACAC AAAAATGGTA GATTCTTTGG TGGAGATGTT GGTTGAAACC
    TCAGATCTTT CTATATTTTG TTTTTATAGT CGTGCTTTTG AGAAGATGTT TCAGCAGTGT
    TTGGAGCTTC CCTCTCAATC AAGATATTCA ATTGCATTCC CACTGCTTTG CACTCATTTT
    ATGAGTTGCA CCCACGAACT GTGCCCAGAA GAGCGACATC ACATCGGAGA TCGTAGTCTT
    TCCTTATGTA ACATGTTTCT GGATGAAATG GCTAAGCAAG CTCGAAATCT TATCACAGAC
    ATTTGCACAG AACAATGTAC GCTGAGTGAT CAGTTGCTGC CAAAGCATTG TGCCAAAACC
    ATCAGCCAAG CAGTGAACAA AAAATCCAAA AAACAGACAG GAAAGAAAGG AGAACCTGAA
    AGGGAGAAAC CAGGAGTAGA AAGCATGAGA AAAAACAGAT TGGTTGTGAC AAACCTGGAC
    AAACTGCACA CTGCACTTTC GGAGCTCTGC TTCTCAATCA ATTATGTACC AAACATGGTG
    GTTTGGGAGC ACACATTTAC CCCAAGAGAA TATTTAACGT CTCACCTGGA AATCCGCTTT
    ACCAAGTCCA TTGTTGGAAT GACGATGTAT AATCAAGCAA CACAGGAGAT TGCAAAGCCT
    TCAGAGCTGT TAACCAGTGT AAGAGCCTAT ATGACGGTCC TCCAGTCAAT AGAAAACTAT
    GTACAGATTG ACATTACACG AGTATTTAAC AATGTTCTGC TTCAGCAAAC TCAGCATTTA
    GATAGTCATG GTGAGCCAAC CATCACCAGT CTGTACACAA ATTGGTATTT GGAAACATTG
    CTAAGGCAAG TCAGCAATGG TCACATAGCC TATTTTCCAG CTATGAAGGC ATTTGTGAAT
    TTGCCAACAG AAAATGAATT AACATTTAAT GCTGAGGAGT ACTCTGACAT ATCAGAAATG
    AGAGCCCTTT CTGAACTCCT GGGACCATAT GGCATGAAGT TCTTAAGCGA AAGTCTGATG
    TGGCACATTT CTTCGCAGGT TGCCGAGCTT AAGAAACTCG TGGTGGAGAA TGTTGAAGTT
    CTAACACAAA TGAGGACCAG CTTCGATAAG CCAGACCAGA TGGCAGCACT CTTCAAAAGA
    TTATCATCTG TTGACAGTGT TTTGAAGAGA ATGACGATTA TTGGTGTCAT CTTGTCTTTT
    CGGTCGTTGG CACAGGAAGC ACTTAGAGAT GTCTTATCCT ACCACATTCC TTTCCTTGTA
    AGTTCCATTG AGGACTTCAA AGATCACATT CCAAGAGAGA CTGACATGAA GGTGGCAATG
    AATGTATATG AACTGTCATC AGCTGCTGGA TTGCCTTGCG AGATTGATCC TGCCTTGGTT
    GTAGCACTGT CCTCCCAAAA ATCAGAAAAC ATCAGTCCAG AAGAAGAATA CAAAATTGCT
    TGCCTTCTCA TGGTCTTCGT GGCAGTCTCC TTGCCAACTT TGGCTAGTAA CGTGATGTCT
    CAGTATAGCC CTGCCATTGA AGGGCACTGC AACAACATTC ATTGCTTGGC AAAAGCTATC
    AACCAGATTG CTGCAGCTTT ATTTACCATC CACAAGGGAA GCATCGAAGA CCGTCTAAAA
    GAATTTTTGG CTCTTGCATC ATCTAGTCTA CTGAAGATCG GCCAGGAGAC GGACAAAACT
    ACTACAAGAA ACAGAGAGTC TGTTTACCTA CTACTGGACA TGATTGTGCA GGAGTCCCCG
    TTCCTGACGA TGGACCTCCT GGAGTCGTGC TTCCCCTACG TCTTGCTGAG AAACGCGTAC
    CACGCTGTCT ACAAACAAAG CGTCACCTCC TCTGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015144835.1
    CDS65..3463
    Translation

    Target ORF information:

    RefSeq Version XM_015289349.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus NCK associated protein 1 (NCKAP1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289349.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCGCGGT CCGTGCTTCA GCCGAGCCAG CAGAAGCTGG CCGAGAAGCT GACCATCCTA 
    AACGACCGGG GCGTCGGCAT GCTCACCCGG CTCTACAACA TCAAGAAGCA AGGACAAGTT
    TGGAAGGCAT GTGGAGACCC CAAAGCCAAA CCATCCTATC TTATTGACAA AAACCTGGAA
    TCTGCAGTCA AATTCATAGT CAGGAAGTTT CCAGCGGTAG AAACACGCAA CAACAATCTT
    GCTCAGCTAC AGAAAGAGAA GTCAGAGATT TTGAAGAACC TGGCGTTGTA CTACTTCACG
    TTTGTTGATG TTATGGAATT TAAGGACCAT GTTTGCGAGC TGCTGAACAC TATTGATGTT
    TGCCAAGTCT TCTTTGATAT TACTGTAAAC TTTGATTTGA CGAAGAACTA CCTGGACTTG
    ATTATAACCT ACACTACACT AATGATACTG CTGTCTCGAA TTGAAGAAAG GAAGGCTATC
    ATTGGATTGT ATAACTATGC TCATGAAATG ACACATGGAG CGAGTGACAG AGAATATCCA
    CGCCTTGGCC AGATGATTGT GGATTATGAA AACCCTTTAA AGAAAATGAT GGAGGAATTT
    GTACCCCACA GTAAATCCCT TTCGGATGCT CTGATTTCAC TCCAGATGGT CTATCCTCGA
    AGAAATCTCT CGGCTGACCA GTGGAGAAAC GCACAACTAC TGAGCCTCAT CAGTGCACCT
    AGTACAATGC TTAACCCTGC ACAGTCTGAC ACGATGCCAT GTGAATACCT CTCTCTGGAT
    GCAATGGAAA AATGGATTAT TTTTGGATTT ATTTTGTGCC ATGGAGTCCT AAATAGTGAT
    GCGACAGCTT TGAACCTTTG GAAACTTGCA CTTCAAAGCA GCTCCTGCTT GGCTCTCTTT
    AGAGATGAAG TATTCCATAT TCACAAAGCT GCAGAAGACT TATTTGTAAA CATACGAGGC
    TACAACAAGC GTATTAATGA CATCAGAGAG TGTAAAGAAG CTGCTGTTTC ACATGCCGGT
    TCGATGCACA GAGAGAGGCG CAAGTTTTTA CGTTCTGCAC TCAAAGAACT GGCGACAGTC
    CTGTCTGATC AGCCAGGCCT GCTGGGCCCC AAGGCACTTT TTGTATTTAT GGCATTATCC
    TTTGCTCGTG ATGAAATCAT TTGGCTTCTT CGTCATGCAG ATAACATGCC AAAGAAGAGT
    GCAGATGACT TCATAGATAA GCACATAGCT GAGCTAATAT TCTACATGGA GGAGCTCAGA
    GCACATGTGC GAAAATACGG ACCTGTGATG CAGAGATACT ACGTACAGTA TCTGTCTGGC
    TTTGATGCAG TGGTCCTAAA TGAACTTGTT CAGAATCTTT CGGTGTGCCC AGAGGATGAA
    TCAATCATCA TGTCCTCTTT TGTAAACACT ATGACTTCGC TAAGTGTGAA GCAGGTTGAG
    GATGGAGAGG TATTTGACTT CAGAGGAATG AGATTAGATT GGTTTAGATT GCAGGCCTAC
    ACCAGTGTCT CTAAAGCTTC TCTTGGACTG GCAGACCACA GGGAGCTAGG CAAAATGATG
    AACACAATCA TCTTCCACAC AAAAATGGTA GATTCTTTGG TGGAGATGTT GGTTGAAACC
    TCAGATCTTT CTATATTTTG TTTTTATAGT CGTGCTTTTG AGAAGATGTT TCAGCAGTGT
    TTGGAGCTTC CCTCTCAATC AAGATATTCA ATTGCATTCC CACTGCTTTG CACTCATTTT
    ATGAGTTGCA CCCACGAACT GTGCCCAGAA GAGCGACATC ACATCGGAGA TCGTAGTCTT
    TCCTTATGTA ACATGTTTCT GGATGAAATG GCTAAGCAAG CTCGAAATCT TATCACAGAC
    ATTTGCACAG AACAATGTAC GCTGAGTGAT CAGTTGCTGC CAAAGCATTG TGCCAAAACC
    ATCAGCCAAG CAGTGAACAA AAAATCCAAA AAACAGACAG GAAAGAAAGG AGAACCTGAA
    AGGGAGAAAC CAGGAGTAGA AAGCATGAGA AAAAACAGAT TGGTTGTGAC AAACCTGGAC
    AAACTGCACA CTGCACTTTC GGAGCTCTGC TTCTCAATCA ATTATGTACC AAACATGGTG
    GTTTGGGAGC ACACATTTAC CCCAAGAGAA TATTTAACGT CTCACCTGGA AATCCGCTTT
    ACCAAGTCCA TTGTTGGAAT GACGATGTAT AATCAAGCAA CACAGGAGAT TGCAAAGCCT
    TCAGAGCTGT TAACCAGTGT AAGAGCCTAT ATGACGGTCC TCCAGTCAAT AGAAAACTAT
    GTACAGATTG ACATTACACG AGTATTTAAC AATGTTCTGC TTCAGCAAAC TCAGCATTTA
    GATAGTCATG GTGAGCCAAC CATCACCAGT CTGTACACAA ATTGGTATTT GGAAACATTG
    CTAAGGCAAG TCAGCAATGG TCACATAGCC TATTTTCCAG CTATGAAGGC ATTTGTGAAT
    TTGCCAACAG AAAATGAATT AACATTTAAT GCTGAGGAGT ACTCTGACAT ATCAGAAATG
    AGAGCCCTTT CTGAACTCCT GGGACCATAT GGCATGAAGT TCTTAAGCGA AAGTCTGATG
    TGGCACATTT CTTCGCAGGT TGCCGAGCTT AAGAAACTCG TGGTGGAGAA TGTTGAAGTT
    CTAACACAAA TGAGGACCAG CTTCGATAAG CCAGACCAGA TGGCAGCACT CTTCAAAAGA
    TTATCATCTG TTGACAGTGT TTTGAAGAGA ATGACGATTA TTGGTGTCAT CTTGTCTTTT
    CGGTCGTTGG CACAGGAAGC ACTTAGAGAT GTCTTATCCT ACCACATTCC TTTCCTTGTA
    AGTTCCATTG AGGACTTCAA AGATCACATT CCAAGAGAGA CTGACATGAA GGTGGCAATG
    AATGTATATG AACTGTCATC AGCTGCTGGA TTGCCTTGCG AGATTGATCC TGCCTTGGTT
    GTAGCACTGT CCTCCCAAAA ATCAGAAAAC ATCAGTCCAG AAGAAGAATA CAAAATTGCT
    TGCCTTCTCA TGGTCTTCGT GGCAGTCTCC TTGCCAACTT TGGCTAGTAA CGTGATGTCT
    CAGTATAGCC CTGCCATTGA AGGGCACTGC AACAACATTC ATTGCTTGGC AAAAGCTATC
    AACCAGATTG CTGCAGCTTT ATTTACCATC CACAAGGGAA GCATCGAAGA CCGTCTAAAA
    GAATTTTTGG CTCTTGCATC ATCTAGTCTA CTGAAGATCG GCCAGGAGAC GGACAAAACT
    ACTACAAGAA ACAGAGAGTC TGTTTACCTA CTACTGGACA TGATTGTGCA GGAGTCCCCG
    TTCCTGACGA TGGACCTCCT GGAGTCGTGC TTCCCCTACG TCTTGCTGAG AAACGCGTAC
    CACGCTGTCT ACAAACAAAG CGTCACCTCC TCTGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa38282
    Clone ID Related Accession (Same CDS sequence) XM_015289349.2 , XM_015289349.1
    Accession Version XM_015289349.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3399bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product nck-associated protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455901.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCGCGGT CCGTGCTTCA GCCGAGCCAG CAGAAGCTGG CCGAGAAGCT GACCATCCTA 
    AACGACCGGG GCGTCGGCAT GCTCACCCGG CTCTACAACA TCAAGAAGCA AGGACAAGTT
    TGGAAGGCAT GTGGAGACCC CAAAGCCAAA CCATCCTATC TTATTGACAA AAACCTGGAA
    TCTGCAGTCA AATTCATAGT CAGGAAGTTT CCAGCGGTAG AAACACGCAA CAACAATCTT
    GCTCAGCTAC AGAAAGAGAA GTCAGAGATT TTGAAGAACC TGGCGTTGTA CTACTTCACG
    TTTGTTGATG TTATGGAATT TAAGGACCAT GTTTGCGAGC TGCTGAACAC TATTGATGTT
    TGCCAAGTCT TCTTTGATAT TACTGTAAAC TTTGATTTGA CGAAGAACTA CCTGGACTTG
    ATTATAACCT ACACTACACT AATGATACTG CTGTCTCGAA TTGAAGAAAG GAAGGCTATC
    ATTGGATTGT ATAACTATGC TCATGAAATG ACACATGGAG CGAGTGACAG AGAATATCCA
    CGCCTTGGCC AGATGATTGT GGATTATGAA AACCCTTTAA AGAAAATGAT GGAGGAATTT
    GTACCCCACA GTAAATCCCT TTCGGATGCT CTGATTTCAC TCCAGATGGT CTATCCTCGA
    AGAAATCTCT CGGCTGACCA GTGGAGAAAC GCACAACTAC TGAGCCTCAT CAGTGCACCT
    AGTACAATGC TTAACCCTGC ACAGTCTGAC ACGATGCCAT GTGAATACCT CTCTCTGGAT
    GCAATGGAAA AATGGATTAT TTTTGGATTT ATTTTGTGCC ATGGAGTCCT AAATAGTGAT
    GCGACAGCTT TGAACCTTTG GAAACTTGCA CTTCAAAGCA GCTCCTGCTT GGCTCTCTTT
    AGAGATGAAG TATTCCATAT TCACAAAGCT GCAGAAGACT TATTTGTAAA CATACGAGGC
    TACAACAAGC GTATTAATGA CATCAGAGAG TGTAAAGAAG CTGCTGTTTC ACATGCCGGT
    TCGATGCACA GAGAGAGGCG CAAGTTTTTA CGTTCTGCAC TCAAAGAACT GGCGACAGTC
    CTGTCTGATC AGCCAGGCCT GCTGGGCCCC AAGGCACTTT TTGTATTTAT GGCATTATCC
    TTTGCTCGTG ATGAAATCAT TTGGCTTCTT CGTCATGCAG ATAACATGCC AAAGAAGAGT
    GCAGATGACT TCATAGATAA GCACATAGCT GAGCTAATAT TCTACATGGA GGAGCTCAGA
    GCACATGTGC GAAAATACGG ACCTGTGATG CAGAGATACT ACGTACAGTA TCTGTCTGGC
    TTTGATGCAG TGGTCCTAAA TGAACTTGTT CAGAATCTTT CGGTGTGCCC AGAGGATGAA
    TCAATCATCA TGTCCTCTTT TGTAAACACT ATGACTTCGC TAAGTGTGAA GCAGGTTGAG
    GATGGAGAGG TATTTGACTT CAGAGGAATG AGATTAGATT GGTTTAGATT GCAGGCCTAC
    ACCAGTGTCT CTAAAGCTTC TCTTGGACTG GCAGACCACA GGGAGCTAGG CAAAATGATG
    AACACAATCA TCTTCCACAC AAAAATGGTA GATTCTTTGG TGGAGATGTT GGTTGAAACC
    TCAGATCTTT CTATATTTTG TTTTTATAGT CGTGCTTTTG AGAAGATGTT TCAGCAGTGT
    TTGGAGCTTC CCTCTCAATC AAGATATTCA ATTGCATTCC CACTGCTTTG CACTCATTTT
    ATGAGTTGCA CCCACGAACT GTGCCCAGAA GAGCGACATC ACATCGGAGA TCGTAGTCTT
    TCCTTATGTA ACATGTTTCT GGATGAAATG GCTAAGCAAG CTCGAAATCT TATCACAGAC
    ATTTGCACAG AACAATGTAC GCTGAGTGAT CAGTTGCTGC CAAAGCATTG TGCCAAAACC
    ATCAGCCAAG CAGTGAACAA AAAATCCAAA AAACAGACAG GAAAGAAAGG AGAACCTGAA
    AGGGAGAAAC CAGGAGTAGA AAGCATGAGA AAAAACAGAT TGGTTGTGAC AAACCTGGAC
    AAACTGCACA CTGCACTTTC GGAGCTCTGC TTCTCAATCA ATTATGTACC AAACATGGTG
    GTTTGGGAGC ACACATTTAC CCCAAGAGAA TATTTAACGT CTCACCTGGA AATCCGCTTT
    ACCAAGTCCA TTGTTGGAAT GACGATGTAT AATCAAGCAA CACAGGAGAT TGCAAAGCCT
    TCAGAGCTGT TAACCAGTGT AAGAGCCTAT ATGACGGTCC TCCAGTCAAT AGAAAACTAT
    GTACAGATTG ACATTACACG AGTATTTAAC AATGTTCTGC TTCAGCAAAC TCAGCATTTA
    GATAGTCATG GTGAGCCAAC CATCACCAGT CTGTACACAA ATTGGTATTT GGAAACATTG
    CTAAGGCAAG TCAGCAATGG TCACATAGCC TATTTTCCAG CTATGAAGGC ATTTGTGAAT
    TTGCCAACAG AAAATGAATT AACATTTAAT GCTGAGGAGT ACTCTGACAT ATCAGAAATG
    AGAGCCCTTT CTGAACTCCT GGGACCATAT GGCATGAAGT TCTTAAGCGA AAGTCTGATG
    TGGCACATTT CTTCGCAGGT TGCCGAGCTT AAGAAACTCG TGGTGGAGAA TGTTGAAGTT
    CTAACACAAA TGAGGACCAG CTTCGATAAG CCAGACCAGA TGGCAGCACT CTTCAAAAGA
    TTATCATCTG TTGACAGTGT TTTGAAGAGA ATGACGATTA TTGGTGTCAT CTTGTCTTTT
    CGGTCGTTGG CACAGGAAGC ACTTAGAGAT GTCTTATCCT ACCACATTCC TTTCCTTGTA
    AGTTCCATTG AGGACTTCAA AGATCACATT CCAAGAGAGA CTGACATGAA GGTGGCAATG
    AATGTATATG AACTGTCATC AGCTGCTGGA TTGCCTTGCG AGATTGATCC TGCCTTGGTT
    GTAGCACTGT CCTCCCAAAA ATCAGAAAAC ATCAGTCCAG AAGAAGAATA CAAAATTGCT
    TGCCTTCTCA TGGTCTTCGT GGCAGTCTCC TTGCCAACTT TGGCTAGTAA CGTGATGTCT
    CAGTATAGCC CTGCCATTGA AGGGCACTGC AACAACATTC ATTGCTTGGC AAAAGCTATC
    AACCAGATTG CTGCAGCTTT ATTTACCATC CACAAGGGAA GCATCGAAGA CCGTCTAAAA
    GAATTTTTGG CTCTTGCATC ATCTAGTCTA CTGAAGATCG GCCAGGAGAC GGACAAAACT
    ACTACAAGAA ACAGAGAGTC TGTTTACCTA CTACTGGACA TGATTGTGCA GGAGTCCCCG
    TTCCTGACGA TGGACCTCCT GGAGTCGTGC TTCCCCTACG TCTTGCTGAG AAACGCGTAC
    CACGCTGTCT ACAAACAAAG CGTCACCTCC TCTGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015144835.1
    CDS53..3451
    Translation

    Target ORF information:

    RefSeq Version XM_015289349.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus NCK-associated protein 1 (NCKAP1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289349.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCGCGGT CCGTGCTTCA GCCGAGCCAG CAGAAGCTGG CCGAGAAGCT GACCATCCTA 
    AACGACCGGG GCGTCGGCAT GCTCACCCGG CTCTACAACA TCAAGAAGCA AGGACAAGTT
    TGGAAGGCAT GTGGAGACCC CAAAGCCAAA CCATCCTATC TTATTGACAA AAACCTGGAA
    TCTGCAGTCA AATTCATAGT CAGGAAGTTT CCAGCGGTAG AAACACGCAA CAACAATCTT
    GCTCAGCTAC AGAAAGAGAA GTCAGAGATT TTGAAGAACC TGGCGTTGTA CTACTTCACG
    TTTGTTGATG TTATGGAATT TAAGGACCAT GTTTGCGAGC TGCTGAACAC TATTGATGTT
    TGCCAAGTCT TCTTTGATAT TACTGTAAAC TTTGATTTGA CGAAGAACTA CCTGGACTTG
    ATTATAACCT ACACTACACT AATGATACTG CTGTCTCGAA TTGAAGAAAG GAAGGCTATC
    ATTGGATTGT ATAACTATGC TCATGAAATG ACACATGGAG CGAGTGACAG AGAATATCCA
    CGCCTTGGCC AGATGATTGT GGATTATGAA AACCCTTTAA AGAAAATGAT GGAGGAATTT
    GTACCCCACA GTAAATCCCT TTCGGATGCT CTGATTTCAC TCCAGATGGT CTATCCTCGA
    AGAAATCTCT CGGCTGACCA GTGGAGAAAC GCACAACTAC TGAGCCTCAT CAGTGCACCT
    AGTACAATGC TTAACCCTGC ACAGTCTGAC ACGATGCCAT GTGAATACCT CTCTCTGGAT
    GCAATGGAAA AATGGATTAT TTTTGGATTT ATTTTGTGCC ATGGAGTCCT AAATAGTGAT
    GCGACAGCTT TGAACCTTTG GAAACTTGCA CTTCAAAGCA GCTCCTGCTT GGCTCTCTTT
    AGAGATGAAG TATTCCATAT TCACAAAGCT GCAGAAGACT TATTTGTAAA CATACGAGGC
    TACAACAAGC GTATTAATGA CATCAGAGAG TGTAAAGAAG CTGCTGTTTC ACATGCCGGT
    TCGATGCACA GAGAGAGGCG CAAGTTTTTA CGTTCTGCAC TCAAAGAACT GGCGACAGTC
    CTGTCTGATC AGCCAGGCCT GCTGGGCCCC AAGGCACTTT TTGTATTTAT GGCATTATCC
    TTTGCTCGTG ATGAAATCAT TTGGCTTCTT CGTCATGCAG ATAACATGCC AAAGAAGAGT
    GCAGATGACT TCATAGATAA GCACATAGCT GAGCTAATAT TCTACATGGA GGAGCTCAGA
    GCACATGTGC GAAAATACGG ACCTGTGATG CAGAGATACT ACGTACAGTA TCTGTCTGGC
    TTTGATGCAG TGGTCCTAAA TGAACTTGTT CAGAATCTTT CGGTGTGCCC AGAGGATGAA
    TCAATCATCA TGTCCTCTTT TGTAAACACT ATGACTTCGC TAAGTGTGAA GCAGGTTGAG
    GATGGAGAGG TATTTGACTT CAGAGGAATG AGATTAGATT GGTTTAGATT GCAGGCCTAC
    ACCAGTGTCT CTAAAGCTTC TCTTGGACTG GCAGACCACA GGGAGCTAGG CAAAATGATG
    AACACAATCA TCTTCCACAC AAAAATGGTA GATTCTTTGG TGGAGATGTT GGTTGAAACC
    TCAGATCTTT CTATATTTTG TTTTTATAGT CGTGCTTTTG AGAAGATGTT TCAGCAGTGT
    TTGGAGCTTC CCTCTCAATC AAGATATTCA ATTGCATTCC CACTGCTTTG CACTCATTTT
    ATGAGTTGCA CCCACGAACT GTGCCCAGAA GAGCGACATC ACATCGGAGA TCGTAGTCTT
    TCCTTATGTA ACATGTTTCT GGATGAAATG GCTAAGCAAG CTCGAAATCT TATCACAGAC
    ATTTGCACAG AACAATGTAC GCTGAGTGAT CAGTTGCTGC CAAAGCATTG TGCCAAAACC
    ATCAGCCAAG CAGTGAACAA AAAATCCAAA AAACAGACAG GAAAGAAAGG AGAACCTGAA
    AGGGAGAAAC CAGGAGTAGA AAGCATGAGA AAAAACAGAT TGGTTGTGAC AAACCTGGAC
    AAACTGCACA CTGCACTTTC GGAGCTCTGC TTCTCAATCA ATTATGTACC AAACATGGTG
    GTTTGGGAGC ACACATTTAC CCCAAGAGAA TATTTAACGT CTCACCTGGA AATCCGCTTT
    ACCAAGTCCA TTGTTGGAAT GACGATGTAT AATCAAGCAA CACAGGAGAT TGCAAAGCCT
    TCAGAGCTGT TAACCAGTGT AAGAGCCTAT ATGACGGTCC TCCAGTCAAT AGAAAACTAT
    GTACAGATTG ACATTACACG AGTATTTAAC AATGTTCTGC TTCAGCAAAC TCAGCATTTA
    GATAGTCATG GTGAGCCAAC CATCACCAGT CTGTACACAA ATTGGTATTT GGAAACATTG
    CTAAGGCAAG TCAGCAATGG TCACATAGCC TATTTTCCAG CTATGAAGGC ATTTGTGAAT
    TTGCCAACAG AAAATGAATT AACATTTAAT GCTGAGGAGT ACTCTGACAT ATCAGAAATG
    AGAGCCCTTT CTGAACTCCT GGGACCATAT GGCATGAAGT TCTTAAGCGA AAGTCTGATG
    TGGCACATTT CTTCGCAGGT TGCCGAGCTT AAGAAACTCG TGGTGGAGAA TGTTGAAGTT
    CTAACACAAA TGAGGACCAG CTTCGATAAG CCAGACCAGA TGGCAGCACT CTTCAAAAGA
    TTATCATCTG TTGACAGTGT TTTGAAGAGA ATGACGATTA TTGGTGTCAT CTTGTCTTTT
    CGGTCGTTGG CACAGGAAGC ACTTAGAGAT GTCTTATCCT ACCACATTCC TTTCCTTGTA
    AGTTCCATTG AGGACTTCAA AGATCACATT CCAAGAGAGA CTGACATGAA GGTGGCAATG
    AATGTATATG AACTGTCATC AGCTGCTGGA TTGCCTTGCG AGATTGATCC TGCCTTGGTT
    GTAGCACTGT CCTCCCAAAA ATCAGAAAAC ATCAGTCCAG AAGAAGAATA CAAAATTGCT
    TGCCTTCTCA TGGTCTTCGT GGCAGTCTCC TTGCCAACTT TGGCTAGTAA CGTGATGTCT
    CAGTATAGCC CTGCCATTGA AGGGCACTGC AACAACATTC ATTGCTTGGC AAAAGCTATC
    AACCAGATTG CTGCAGCTTT ATTTACCATC CACAAGGGAA GCATCGAAGA CCGTCTAAAA
    GAATTTTTGG CTCTTGCATC ATCTAGTCTA CTGAAGATCG GCCAGGAGAC GGACAAAACT
    ACTACAAGAA ACAGAGAGTC TGTTTACCTA CTACTGGACA TGATTGTGCA GGAGTCCCCG
    TTCCTGACGA TGGACCTCCT GGAGTCGTGC TTCCCCTACG TCTTGCTGAG AAACGCGTAC
    CACGCTGTCT ACAAACAAAG CGTCACCTCC TCTGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa38283
    Clone ID Related Accession (Same CDS sequence) XM_015289350.2 , XM_015289350.1
    Accession Version XM_015289350.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3387bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product nck-associated protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289350.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCGCGGT CCGTGCTTCA GCCGAGCCAG CAGAAGCTGG CCGAGAAGCT GACCATCCTA 
    AACGACCGGG GCGTCGGCAT GCTCACCCGG CTCTACAACA TCAAGAAGGC ATGTGGAGAC
    CCCAAAGCCA AACCATCCTA TCTTATTGAC AAAAACCTGG AATCTGCAGT CAAATTCATA
    GTCAGGAAGT TTCCAGCGGT AGAAACACGC AACAACAATC AACAGCTTGC TCAGCTACAG
    AAAGAGAAGT CAGAGATTTT GAAGAACCTG GCGTTGTACT ACTTCACGTT TGTTGATGTT
    ATGGAATTTA AGGACCATGT TTGCGAGCTG CTGAACACTA TTGATGTTTG CCAAGTCTTC
    TTTGATATTA CTGTAAACTT TGATTTGACG AAGAACTACC TGGACTTGAT TATAACCTAC
    ACTACACTAA TGATACTGCT GTCTCGAATT GAAGAAAGGA AGGCTATCAT TGGATTGTAT
    AACTATGCTC ATGAAATGAC ACATGGAGCG AGTGACAGAG AATATCCACG CCTTGGCCAG
    ATGATTGTGG ATTATGAAAA CCCTTTAAAG AAAATGATGG AGGAATTTGT ACCCCACAGT
    AAATCCCTTT CGGATGCTCT GATTTCACTC CAGATGGTCT ATCCTCGAAG AAATCTCTCG
    GCTGACCAGT GGAGAAACGC ACAACTACTG AGCCTCATCA GTGCACCTAG TACAATGCTT
    AACCCTGCAC AGTCTGACAC GATGCCATGT GAATACCTCT CTCTGGATGC AATGGAAAAA
    TGGATTATTT TTGGATTTAT TTTGTGCCAT GGAGTCCTAA ATAGTGATGC GACAGCTTTG
    AACCTTTGGA AACTTGCACT TCAAAGCAGC TCCTGCTTGG CTCTCTTTAG AGATGAAGTA
    TTCCATATTC ACAAAGCTGC AGAAGACTTA TTTGTAAACA TACGAGGCTA CAACAAGCGT
    ATTAATGACA TCAGAGAGTG TAAAGAAGCT GCTGTTTCAC ATGCCGGTTC GATGCACAGA
    GAGAGGCGCA AGTTTTTACG TTCTGCACTC AAAGAACTGG CGACAGTCCT GTCTGATCAG
    CCAGGCCTGC TGGGCCCCAA GGCACTTTTT GTATTTATGG CATTATCCTT TGCTCGTGAT
    GAAATCATTT GGCTTCTTCG TCATGCAGAT AACATGCCAA AGAAGAGTGC AGATGACTTC
    ATAGATAAGC ACATAGCTGA GCTAATATTC TACATGGAGG AGCTCAGAGC ACATGTGCGA
    AAATACGGAC CTGTGATGCA GAGATACTAC GTACAGTATC TGTCTGGCTT TGATGCAGTG
    GTCCTAAATG AACTTGTTCA GAATCTTTCG GTGTGCCCAG AGGATGAATC AATCATCATG
    TCCTCTTTTG TAAACACTAT GACTTCGCTA AGTGTGAAGC AGGTTGAGGA TGGAGAGGTA
    TTTGACTTCA GAGGAATGAG ATTAGATTGG TTTAGATTGC AGGCCTACAC CAGTGTCTCT
    AAAGCTTCTC TTGGACTGGC AGACCACAGG GAGCTAGGCA AAATGATGAA CACAATCATC
    TTCCACACAA AAATGGTAGA TTCTTTGGTG GAGATGTTGG TTGAAACCTC AGATCTTTCT
    ATATTTTGTT TTTATAGTCG TGCTTTTGAG AAGATGTTTC AGCAGTGTTT GGAGCTTCCC
    TCTCAATCAA GATATTCAAT TGCATTCCCA CTGCTTTGCA CTCATTTTAT GAGTTGCACC
    CACGAACTGT GCCCAGAAGA GCGACATCAC ATCGGAGATC GTAGTCTTTC CTTATGTAAC
    ATGTTTCTGG ATGAAATGGC TAAGCAAGCT CGAAATCTTA TCACAGACAT TTGCACAGAA
    CAATGTACGC TGAGTGATCA GTTGCTGCCA AAGCATTGTG CCAAAACCAT CAGCCAAGCA
    GTGAACAAAA AATCCAAAAA ACAGACAGGA AAGAAAGGAG AACCTGAAAG GGAGAAACCA
    GGAGTAGAAA GCATGAGAAA AAACAGATTG GTTGTGACAA ACCTGGACAA ACTGCACACT
    GCACTTTCGG AGCTCTGCTT CTCAATCAAT TATGTACCAA ACATGGTGGT TTGGGAGCAC
    ACATTTACCC CAAGAGAATA TTTAACGTCT CACCTGGAAA TCCGCTTTAC CAAGTCCATT
    GTTGGAATGA CGATGTATAA TCAAGCAACA CAGGAGATTG CAAAGCCTTC AGAGCTGTTA
    ACCAGTGTAA GAGCCTATAT GACGGTCCTC CAGTCAATAG AAAACTATGT ACAGATTGAC
    ATTACACGAG TATTTAACAA TGTTCTGCTT CAGCAAACTC AGCATTTAGA TAGTCATGGT
    GAGCCAACCA TCACCAGTCT GTACACAAAT TGGTATTTGG AAACATTGCT AAGGCAAGTC
    AGCAATGGTC ACATAGCCTA TTTTCCAGCT ATGAAGGCAT TTGTGAATTT GCCAACAGAA
    AATGAATTAA CATTTAATGC TGAGGAGTAC TCTGACATAT CAGAAATGAG AGCCCTTTCT
    GAACTCCTGG GACCATATGG CATGAAGTTC TTAAGCGAAA GTCTGATGTG GCACATTTCT
    TCGCAGGTTG CCGAGCTTAA GAAACTCGTG GTGGAGAATG TTGAAGTTCT AACACAAATG
    AGGACCAGCT TCGATAAGCC AGACCAGATG GCAGCACTCT TCAAAAGATT ATCATCTGTT
    GACAGTGTTT TGAAGAGAAT GACGATTATT GGTGTCATCT TGTCTTTTCG GTCGTTGGCA
    CAGGAAGCAC TTAGAGATGT CTTATCCTAC CACATTCCTT TCCTTGTAAG TTCCATTGAG
    GACTTCAAAG ATCACATTCC AAGAGAGACT GACATGAAGG TGGCAATGAA TGTATATGAA
    CTGTCATCAG CTGCTGGATT GCCTTGCGAG ATTGATCCTG CCTTGGTTGT AGCACTGTCC
    TCCCAAAAAT CAGAAAACAT CAGTCCAGAA GAAGAATACA AAATTGCTTG CCTTCTCATG
    GTCTTCGTGG CAGTCTCCTT GCCAACTTTG GCTAGTAACG TGATGTCTCA GTATAGCCCT
    GCCATTGAAG GGCACTGCAA CAACATTCAT TGCTTGGCAA AAGCTATCAA CCAGATTGCT
    GCAGCTTTAT TTACCATCCA CAAGGGAAGC ATCGAAGACC GTCTAAAAGA ATTTTTGGCT
    CTTGCATCAT CTAGTCTACT GAAGATCGGC CAGGAGACGG ACAAAACTAC TACAAGAAAC
    AGAGAGTCTG TTTACCTACT ACTGGACATG ATTGTGCAGG AGTCCCCGTT CCTGACGATG
    GACCTCCTGG AGTCGTGCTT CCCCTACGTC TTGCTGAGAA ACGCGTACCA CGCTGTCTAC
    AAACAAAGCG TCACCTCCTC TGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015144836.1
    CDS64..3450
    Translation

    Target ORF information:

    RefSeq Version XM_015289350.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus NCK associated protein 1 (NCKAP1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289350.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCGCGGT CCGTGCTTCA GCCGAGCCAG CAGAAGCTGG CCGAGAAGCT GACCATCCTA 
    AACGACCGGG GCGTCGGCAT GCTCACCCGG CTCTACAACA TCAAGAAGGC ATGTGGAGAC
    CCCAAAGCCA AACCATCCTA TCTTATTGAC AAAAACCTGG AATCTGCAGT CAAATTCATA
    GTCAGGAAGT TTCCAGCGGT AGAAACACGC AACAACAATC AACAGCTTGC TCAGCTACAG
    AAAGAGAAGT CAGAGATTTT GAAGAACCTG GCGTTGTACT ACTTCACGTT TGTTGATGTT
    ATGGAATTTA AGGACCATGT TTGCGAGCTG CTGAACACTA TTGATGTTTG CCAAGTCTTC
    TTTGATATTA CTGTAAACTT TGATTTGACG AAGAACTACC TGGACTTGAT TATAACCTAC
    ACTACACTAA TGATACTGCT GTCTCGAATT GAAGAAAGGA AGGCTATCAT TGGATTGTAT
    AACTATGCTC ATGAAATGAC ACATGGAGCG AGTGACAGAG AATATCCACG CCTTGGCCAG
    ATGATTGTGG ATTATGAAAA CCCTTTAAAG AAAATGATGG AGGAATTTGT ACCCCACAGT
    AAATCCCTTT CGGATGCTCT GATTTCACTC CAGATGGTCT ATCCTCGAAG AAATCTCTCG
    GCTGACCAGT GGAGAAACGC ACAACTACTG AGCCTCATCA GTGCACCTAG TACAATGCTT
    AACCCTGCAC AGTCTGACAC GATGCCATGT GAATACCTCT CTCTGGATGC AATGGAAAAA
    TGGATTATTT TTGGATTTAT TTTGTGCCAT GGAGTCCTAA ATAGTGATGC GACAGCTTTG
    AACCTTTGGA AACTTGCACT TCAAAGCAGC TCCTGCTTGG CTCTCTTTAG AGATGAAGTA
    TTCCATATTC ACAAAGCTGC AGAAGACTTA TTTGTAAACA TACGAGGCTA CAACAAGCGT
    ATTAATGACA TCAGAGAGTG TAAAGAAGCT GCTGTTTCAC ATGCCGGTTC GATGCACAGA
    GAGAGGCGCA AGTTTTTACG TTCTGCACTC AAAGAACTGG CGACAGTCCT GTCTGATCAG
    CCAGGCCTGC TGGGCCCCAA GGCACTTTTT GTATTTATGG CATTATCCTT TGCTCGTGAT
    GAAATCATTT GGCTTCTTCG TCATGCAGAT AACATGCCAA AGAAGAGTGC AGATGACTTC
    ATAGATAAGC ACATAGCTGA GCTAATATTC TACATGGAGG AGCTCAGAGC ACATGTGCGA
    AAATACGGAC CTGTGATGCA GAGATACTAC GTACAGTATC TGTCTGGCTT TGATGCAGTG
    GTCCTAAATG AACTTGTTCA GAATCTTTCG GTGTGCCCAG AGGATGAATC AATCATCATG
    TCCTCTTTTG TAAACACTAT GACTTCGCTA AGTGTGAAGC AGGTTGAGGA TGGAGAGGTA
    TTTGACTTCA GAGGAATGAG ATTAGATTGG TTTAGATTGC AGGCCTACAC CAGTGTCTCT
    AAAGCTTCTC TTGGACTGGC AGACCACAGG GAGCTAGGCA AAATGATGAA CACAATCATC
    TTCCACACAA AAATGGTAGA TTCTTTGGTG GAGATGTTGG TTGAAACCTC AGATCTTTCT
    ATATTTTGTT TTTATAGTCG TGCTTTTGAG AAGATGTTTC AGCAGTGTTT GGAGCTTCCC
    TCTCAATCAA GATATTCAAT TGCATTCCCA CTGCTTTGCA CTCATTTTAT GAGTTGCACC
    CACGAACTGT GCCCAGAAGA GCGACATCAC ATCGGAGATC GTAGTCTTTC CTTATGTAAC
    ATGTTTCTGG ATGAAATGGC TAAGCAAGCT CGAAATCTTA TCACAGACAT TTGCACAGAA
    CAATGTACGC TGAGTGATCA GTTGCTGCCA AAGCATTGTG CCAAAACCAT CAGCCAAGCA
    GTGAACAAAA AATCCAAAAA ACAGACAGGA AAGAAAGGAG AACCTGAAAG GGAGAAACCA
    GGAGTAGAAA GCATGAGAAA AAACAGATTG GTTGTGACAA ACCTGGACAA ACTGCACACT
    GCACTTTCGG AGCTCTGCTT CTCAATCAAT TATGTACCAA ACATGGTGGT TTGGGAGCAC
    ACATTTACCC CAAGAGAATA TTTAACGTCT CACCTGGAAA TCCGCTTTAC CAAGTCCATT
    GTTGGAATGA CGATGTATAA TCAAGCAACA CAGGAGATTG CAAAGCCTTC AGAGCTGTTA
    ACCAGTGTAA GAGCCTATAT GACGGTCCTC CAGTCAATAG AAAACTATGT ACAGATTGAC
    ATTACACGAG TATTTAACAA TGTTCTGCTT CAGCAAACTC AGCATTTAGA TAGTCATGGT
    GAGCCAACCA TCACCAGTCT GTACACAAAT TGGTATTTGG AAACATTGCT AAGGCAAGTC
    AGCAATGGTC ACATAGCCTA TTTTCCAGCT ATGAAGGCAT TTGTGAATTT GCCAACAGAA
    AATGAATTAA CATTTAATGC TGAGGAGTAC TCTGACATAT CAGAAATGAG AGCCCTTTCT
    GAACTCCTGG GACCATATGG CATGAAGTTC TTAAGCGAAA GTCTGATGTG GCACATTTCT
    TCGCAGGTTG CCGAGCTTAA GAAACTCGTG GTGGAGAATG TTGAAGTTCT AACACAAATG
    AGGACCAGCT TCGATAAGCC AGACCAGATG GCAGCACTCT TCAAAAGATT ATCATCTGTT
    GACAGTGTTT TGAAGAGAAT GACGATTATT GGTGTCATCT TGTCTTTTCG GTCGTTGGCA
    CAGGAAGCAC TTAGAGATGT CTTATCCTAC CACATTCCTT TCCTTGTAAG TTCCATTGAG
    GACTTCAAAG ATCACATTCC AAGAGAGACT GACATGAAGG TGGCAATGAA TGTATATGAA
    CTGTCATCAG CTGCTGGATT GCCTTGCGAG ATTGATCCTG CCTTGGTTGT AGCACTGTCC
    TCCCAAAAAT CAGAAAACAT CAGTCCAGAA GAAGAATACA AAATTGCTTG CCTTCTCATG
    GTCTTCGTGG CAGTCTCCTT GCCAACTTTG GCTAGTAACG TGATGTCTCA GTATAGCCCT
    GCCATTGAAG GGCACTGCAA CAACATTCAT TGCTTGGCAA AAGCTATCAA CCAGATTGCT
    GCAGCTTTAT TTACCATCCA CAAGGGAAGC ATCGAAGACC GTCTAAAAGA ATTTTTGGCT
    CTTGCATCAT CTAGTCTACT GAAGATCGGC CAGGAGACGG ACAAAACTAC TACAAGAAAC
    AGAGAGTCTG TTTACCTACT ACTGGACATG ATTGTGCAGG AGTCCCCGTT CCTGACGATG
    GACCTCCTGG AGTCGTGCTT CCCCTACGTC TTGCTGAGAA ACGCGTACCA CGCTGTCTAC
    AAACAAAGCG TCACCTCCTC TGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa38283
    Clone ID Related Accession (Same CDS sequence) XM_015289350.2 , XM_015289350.1
    Accession Version XM_015289350.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3387bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product nck-associated protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455901.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCGCGGT CCGTGCTTCA GCCGAGCCAG CAGAAGCTGG CCGAGAAGCT GACCATCCTA 
    AACGACCGGG GCGTCGGCAT GCTCACCCGG CTCTACAACA TCAAGAAGGC ATGTGGAGAC
    CCCAAAGCCA AACCATCCTA TCTTATTGAC AAAAACCTGG AATCTGCAGT CAAATTCATA
    GTCAGGAAGT TTCCAGCGGT AGAAACACGC AACAACAATC AACAGCTTGC TCAGCTACAG
    AAAGAGAAGT CAGAGATTTT GAAGAACCTG GCGTTGTACT ACTTCACGTT TGTTGATGTT
    ATGGAATTTA AGGACCATGT TTGCGAGCTG CTGAACACTA TTGATGTTTG CCAAGTCTTC
    TTTGATATTA CTGTAAACTT TGATTTGACG AAGAACTACC TGGACTTGAT TATAACCTAC
    ACTACACTAA TGATACTGCT GTCTCGAATT GAAGAAAGGA AGGCTATCAT TGGATTGTAT
    AACTATGCTC ATGAAATGAC ACATGGAGCG AGTGACAGAG AATATCCACG CCTTGGCCAG
    ATGATTGTGG ATTATGAAAA CCCTTTAAAG AAAATGATGG AGGAATTTGT ACCCCACAGT
    AAATCCCTTT CGGATGCTCT GATTTCACTC CAGATGGTCT ATCCTCGAAG AAATCTCTCG
    GCTGACCAGT GGAGAAACGC ACAACTACTG AGCCTCATCA GTGCACCTAG TACAATGCTT
    AACCCTGCAC AGTCTGACAC GATGCCATGT GAATACCTCT CTCTGGATGC AATGGAAAAA
    TGGATTATTT TTGGATTTAT TTTGTGCCAT GGAGTCCTAA ATAGTGATGC GACAGCTTTG
    AACCTTTGGA AACTTGCACT TCAAAGCAGC TCCTGCTTGG CTCTCTTTAG AGATGAAGTA
    TTCCATATTC ACAAAGCTGC AGAAGACTTA TTTGTAAACA TACGAGGCTA CAACAAGCGT
    ATTAATGACA TCAGAGAGTG TAAAGAAGCT GCTGTTTCAC ATGCCGGTTC GATGCACAGA
    GAGAGGCGCA AGTTTTTACG TTCTGCACTC AAAGAACTGG CGACAGTCCT GTCTGATCAG
    CCAGGCCTGC TGGGCCCCAA GGCACTTTTT GTATTTATGG CATTATCCTT TGCTCGTGAT
    GAAATCATTT GGCTTCTTCG TCATGCAGAT AACATGCCAA AGAAGAGTGC AGATGACTTC
    ATAGATAAGC ACATAGCTGA GCTAATATTC TACATGGAGG AGCTCAGAGC ACATGTGCGA
    AAATACGGAC CTGTGATGCA GAGATACTAC GTACAGTATC TGTCTGGCTT TGATGCAGTG
    GTCCTAAATG AACTTGTTCA GAATCTTTCG GTGTGCCCAG AGGATGAATC AATCATCATG
    TCCTCTTTTG TAAACACTAT GACTTCGCTA AGTGTGAAGC AGGTTGAGGA TGGAGAGGTA
    TTTGACTTCA GAGGAATGAG ATTAGATTGG TTTAGATTGC AGGCCTACAC CAGTGTCTCT
    AAAGCTTCTC TTGGACTGGC AGACCACAGG GAGCTAGGCA AAATGATGAA CACAATCATC
    TTCCACACAA AAATGGTAGA TTCTTTGGTG GAGATGTTGG TTGAAACCTC AGATCTTTCT
    ATATTTTGTT TTTATAGTCG TGCTTTTGAG AAGATGTTTC AGCAGTGTTT GGAGCTTCCC
    TCTCAATCAA GATATTCAAT TGCATTCCCA CTGCTTTGCA CTCATTTTAT GAGTTGCACC
    CACGAACTGT GCCCAGAAGA GCGACATCAC ATCGGAGATC GTAGTCTTTC CTTATGTAAC
    ATGTTTCTGG ATGAAATGGC TAAGCAAGCT CGAAATCTTA TCACAGACAT TTGCACAGAA
    CAATGTACGC TGAGTGATCA GTTGCTGCCA AAGCATTGTG CCAAAACCAT CAGCCAAGCA
    GTGAACAAAA AATCCAAAAA ACAGACAGGA AAGAAAGGAG AACCTGAAAG GGAGAAACCA
    GGAGTAGAAA GCATGAGAAA AAACAGATTG GTTGTGACAA ACCTGGACAA ACTGCACACT
    GCACTTTCGG AGCTCTGCTT CTCAATCAAT TATGTACCAA ACATGGTGGT TTGGGAGCAC
    ACATTTACCC CAAGAGAATA TTTAACGTCT CACCTGGAAA TCCGCTTTAC CAAGTCCATT
    GTTGGAATGA CGATGTATAA TCAAGCAACA CAGGAGATTG CAAAGCCTTC AGAGCTGTTA
    ACCAGTGTAA GAGCCTATAT GACGGTCCTC CAGTCAATAG AAAACTATGT ACAGATTGAC
    ATTACACGAG TATTTAACAA TGTTCTGCTT CAGCAAACTC AGCATTTAGA TAGTCATGGT
    GAGCCAACCA TCACCAGTCT GTACACAAAT TGGTATTTGG AAACATTGCT AAGGCAAGTC
    AGCAATGGTC ACATAGCCTA TTTTCCAGCT ATGAAGGCAT TTGTGAATTT GCCAACAGAA
    AATGAATTAA CATTTAATGC TGAGGAGTAC TCTGACATAT CAGAAATGAG AGCCCTTTCT
    GAACTCCTGG GACCATATGG CATGAAGTTC TTAAGCGAAA GTCTGATGTG GCACATTTCT
    TCGCAGGTTG CCGAGCTTAA GAAACTCGTG GTGGAGAATG TTGAAGTTCT AACACAAATG
    AGGACCAGCT TCGATAAGCC AGACCAGATG GCAGCACTCT TCAAAAGATT ATCATCTGTT
    GACAGTGTTT TGAAGAGAAT GACGATTATT GGTGTCATCT TGTCTTTTCG GTCGTTGGCA
    CAGGAAGCAC TTAGAGATGT CTTATCCTAC CACATTCCTT TCCTTGTAAG TTCCATTGAG
    GACTTCAAAG ATCACATTCC AAGAGAGACT GACATGAAGG TGGCAATGAA TGTATATGAA
    CTGTCATCAG CTGCTGGATT GCCTTGCGAG ATTGATCCTG CCTTGGTTGT AGCACTGTCC
    TCCCAAAAAT CAGAAAACAT CAGTCCAGAA GAAGAATACA AAATTGCTTG CCTTCTCATG
    GTCTTCGTGG CAGTCTCCTT GCCAACTTTG GCTAGTAACG TGATGTCTCA GTATAGCCCT
    GCCATTGAAG GGCACTGCAA CAACATTCAT TGCTTGGCAA AAGCTATCAA CCAGATTGCT
    GCAGCTTTAT TTACCATCCA CAAGGGAAGC ATCGAAGACC GTCTAAAAGA ATTTTTGGCT
    CTTGCATCAT CTAGTCTACT GAAGATCGGC CAGGAGACGG ACAAAACTAC TACAAGAAAC
    AGAGAGTCTG TTTACCTACT ACTGGACATG ATTGTGCAGG AGTCCCCGTT CCTGACGATG
    GACCTCCTGG AGTCGTGCTT CCCCTACGTC TTGCTGAGAA ACGCGTACCA CGCTGTCTAC
    AAACAAAGCG TCACCTCCTC TGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015144836.1
    CDS52..3438
    Translation

    Target ORF information:

    RefSeq Version XM_015289350.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus NCK-associated protein 1 (NCKAP1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289350.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCGCGGT CCGTGCTTCA GCCGAGCCAG CAGAAGCTGG CCGAGAAGCT GACCATCCTA 
    AACGACCGGG GCGTCGGCAT GCTCACCCGG CTCTACAACA TCAAGAAGGC ATGTGGAGAC
    CCCAAAGCCA AACCATCCTA TCTTATTGAC AAAAACCTGG AATCTGCAGT CAAATTCATA
    GTCAGGAAGT TTCCAGCGGT AGAAACACGC AACAACAATC AACAGCTTGC TCAGCTACAG
    AAAGAGAAGT CAGAGATTTT GAAGAACCTG GCGTTGTACT ACTTCACGTT TGTTGATGTT
    ATGGAATTTA AGGACCATGT TTGCGAGCTG CTGAACACTA TTGATGTTTG CCAAGTCTTC
    TTTGATATTA CTGTAAACTT TGATTTGACG AAGAACTACC TGGACTTGAT TATAACCTAC
    ACTACACTAA TGATACTGCT GTCTCGAATT GAAGAAAGGA AGGCTATCAT TGGATTGTAT
    AACTATGCTC ATGAAATGAC ACATGGAGCG AGTGACAGAG AATATCCACG CCTTGGCCAG
    ATGATTGTGG ATTATGAAAA CCCTTTAAAG AAAATGATGG AGGAATTTGT ACCCCACAGT
    AAATCCCTTT CGGATGCTCT GATTTCACTC CAGATGGTCT ATCCTCGAAG AAATCTCTCG
    GCTGACCAGT GGAGAAACGC ACAACTACTG AGCCTCATCA GTGCACCTAG TACAATGCTT
    AACCCTGCAC AGTCTGACAC GATGCCATGT GAATACCTCT CTCTGGATGC AATGGAAAAA
    TGGATTATTT TTGGATTTAT TTTGTGCCAT GGAGTCCTAA ATAGTGATGC GACAGCTTTG
    AACCTTTGGA AACTTGCACT TCAAAGCAGC TCCTGCTTGG CTCTCTTTAG AGATGAAGTA
    TTCCATATTC ACAAAGCTGC AGAAGACTTA TTTGTAAACA TACGAGGCTA CAACAAGCGT
    ATTAATGACA TCAGAGAGTG TAAAGAAGCT GCTGTTTCAC ATGCCGGTTC GATGCACAGA
    GAGAGGCGCA AGTTTTTACG TTCTGCACTC AAAGAACTGG CGACAGTCCT GTCTGATCAG
    CCAGGCCTGC TGGGCCCCAA GGCACTTTTT GTATTTATGG CATTATCCTT TGCTCGTGAT
    GAAATCATTT GGCTTCTTCG TCATGCAGAT AACATGCCAA AGAAGAGTGC AGATGACTTC
    ATAGATAAGC ACATAGCTGA GCTAATATTC TACATGGAGG AGCTCAGAGC ACATGTGCGA
    AAATACGGAC CTGTGATGCA GAGATACTAC GTACAGTATC TGTCTGGCTT TGATGCAGTG
    GTCCTAAATG AACTTGTTCA GAATCTTTCG GTGTGCCCAG AGGATGAATC AATCATCATG
    TCCTCTTTTG TAAACACTAT GACTTCGCTA AGTGTGAAGC AGGTTGAGGA TGGAGAGGTA
    TTTGACTTCA GAGGAATGAG ATTAGATTGG TTTAGATTGC AGGCCTACAC CAGTGTCTCT
    AAAGCTTCTC TTGGACTGGC AGACCACAGG GAGCTAGGCA AAATGATGAA CACAATCATC
    TTCCACACAA AAATGGTAGA TTCTTTGGTG GAGATGTTGG TTGAAACCTC AGATCTTTCT
    ATATTTTGTT TTTATAGTCG TGCTTTTGAG AAGATGTTTC AGCAGTGTTT GGAGCTTCCC
    TCTCAATCAA GATATTCAAT TGCATTCCCA CTGCTTTGCA CTCATTTTAT GAGTTGCACC
    CACGAACTGT GCCCAGAAGA GCGACATCAC ATCGGAGATC GTAGTCTTTC CTTATGTAAC
    ATGTTTCTGG ATGAAATGGC TAAGCAAGCT CGAAATCTTA TCACAGACAT TTGCACAGAA
    CAATGTACGC TGAGTGATCA GTTGCTGCCA AAGCATTGTG CCAAAACCAT CAGCCAAGCA
    GTGAACAAAA AATCCAAAAA ACAGACAGGA AAGAAAGGAG AACCTGAAAG GGAGAAACCA
    GGAGTAGAAA GCATGAGAAA AAACAGATTG GTTGTGACAA ACCTGGACAA ACTGCACACT
    GCACTTTCGG AGCTCTGCTT CTCAATCAAT TATGTACCAA ACATGGTGGT TTGGGAGCAC
    ACATTTACCC CAAGAGAATA TTTAACGTCT CACCTGGAAA TCCGCTTTAC CAAGTCCATT
    GTTGGAATGA CGATGTATAA TCAAGCAACA CAGGAGATTG CAAAGCCTTC AGAGCTGTTA
    ACCAGTGTAA GAGCCTATAT GACGGTCCTC CAGTCAATAG AAAACTATGT ACAGATTGAC
    ATTACACGAG TATTTAACAA TGTTCTGCTT CAGCAAACTC AGCATTTAGA TAGTCATGGT
    GAGCCAACCA TCACCAGTCT GTACACAAAT TGGTATTTGG AAACATTGCT AAGGCAAGTC
    AGCAATGGTC ACATAGCCTA TTTTCCAGCT ATGAAGGCAT TTGTGAATTT GCCAACAGAA
    AATGAATTAA CATTTAATGC TGAGGAGTAC TCTGACATAT CAGAAATGAG AGCCCTTTCT
    GAACTCCTGG GACCATATGG CATGAAGTTC TTAAGCGAAA GTCTGATGTG GCACATTTCT
    TCGCAGGTTG CCGAGCTTAA GAAACTCGTG GTGGAGAATG TTGAAGTTCT AACACAAATG
    AGGACCAGCT TCGATAAGCC AGACCAGATG GCAGCACTCT TCAAAAGATT ATCATCTGTT
    GACAGTGTTT TGAAGAGAAT GACGATTATT GGTGTCATCT TGTCTTTTCG GTCGTTGGCA
    CAGGAAGCAC TTAGAGATGT CTTATCCTAC CACATTCCTT TCCTTGTAAG TTCCATTGAG
    GACTTCAAAG ATCACATTCC AAGAGAGACT GACATGAAGG TGGCAATGAA TGTATATGAA
    CTGTCATCAG CTGCTGGATT GCCTTGCGAG ATTGATCCTG CCTTGGTTGT AGCACTGTCC
    TCCCAAAAAT CAGAAAACAT CAGTCCAGAA GAAGAATACA AAATTGCTTG CCTTCTCATG
    GTCTTCGTGG CAGTCTCCTT GCCAACTTTG GCTAGTAACG TGATGTCTCA GTATAGCCCT
    GCCATTGAAG GGCACTGCAA CAACATTCAT TGCTTGGCAA AAGCTATCAA CCAGATTGCT
    GCAGCTTTAT TTACCATCCA CAAGGGAAGC ATCGAAGACC GTCTAAAAGA ATTTTTGGCT
    CTTGCATCAT CTAGTCTACT GAAGATCGGC CAGGAGACGG ACAAAACTAC TACAAGAAAC
    AGAGAGTCTG TTTACCTACT ACTGGACATG ATTGTGCAGG AGTCCCCGTT CCTGACGATG
    GACCTCCTGG AGTCGTGCTT CCCCTACGTC TTGCTGAGAA ACGCGTACCA CGCTGTCTAC
    AAACAAAGCG TCACCTCCTC TGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa38284
    Clone ID Related Accession (Same CDS sequence) XM_015289351.1 , XM_015289351.2
    Accession Version XM_015289351.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3381bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product nck-associated protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455901.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCGCGGT CCGTGCTTCA GCCGAGCCAG CAGAAGCTGG CCGAGAAGCT GACCATCCTA 
    AACGACCGGG GCGTCGGCAT GCTCACCCGG CTCTACAACA TCAAGAAGGC ATGTGGAGAC
    CCCAAAGCCA AACCATCCTA TCTTATTGAC AAAAACCTGG AATCTGCAGT CAAATTCATA
    GTCAGGAAGT TTCCAGCGGT AGAAACACGC AACAACAATC TTGCTCAGCT ACAGAAAGAG
    AAGTCAGAGA TTTTGAAGAA CCTGGCGTTG TACTACTTCA CGTTTGTTGA TGTTATGGAA
    TTTAAGGACC ATGTTTGCGA GCTGCTGAAC ACTATTGATG TTTGCCAAGT CTTCTTTGAT
    ATTACTGTAA ACTTTGATTT GACGAAGAAC TACCTGGACT TGATTATAAC CTACACTACA
    CTAATGATAC TGCTGTCTCG AATTGAAGAA AGGAAGGCTA TCATTGGATT GTATAACTAT
    GCTCATGAAA TGACACATGG AGCGAGTGAC AGAGAATATC CACGCCTTGG CCAGATGATT
    GTGGATTATG AAAACCCTTT AAAGAAAATG ATGGAGGAAT TTGTACCCCA CAGTAAATCC
    CTTTCGGATG CTCTGATTTC ACTCCAGATG GTCTATCCTC GAAGAAATCT CTCGGCTGAC
    CAGTGGAGAA ACGCACAACT ACTGAGCCTC ATCAGTGCAC CTAGTACAAT GCTTAACCCT
    GCACAGTCTG ACACGATGCC ATGTGAATAC CTCTCTCTGG ATGCAATGGA AAAATGGATT
    ATTTTTGGAT TTATTTTGTG CCATGGAGTC CTAAATAGTG ATGCGACAGC TTTGAACCTT
    TGGAAACTTG CACTTCAAAG CAGCTCCTGC TTGGCTCTCT TTAGAGATGA AGTATTCCAT
    ATTCACAAAG CTGCAGAAGA CTTATTTGTA AACATACGAG GCTACAACAA GCGTATTAAT
    GACATCAGAG AGTGTAAAGA AGCTGCTGTT TCACATGCCG GTTCGATGCA CAGAGAGAGG
    CGCAAGTTTT TACGTTCTGC ACTCAAAGAA CTGGCGACAG TCCTGTCTGA TCAGCCAGGC
    CTGCTGGGCC CCAAGGCACT TTTTGTATTT ATGGCATTAT CCTTTGCTCG TGATGAAATC
    ATTTGGCTTC TTCGTCATGC AGATAACATG CCAAAGAAGA GTGCAGATGA CTTCATAGAT
    AAGCACATAG CTGAGCTAAT ATTCTACATG GAGGAGCTCA GAGCACATGT GCGAAAATAC
    GGACCTGTGA TGCAGAGATA CTACGTACAG TATCTGTCTG GCTTTGATGC AGTGGTCCTA
    AATGAACTTG TTCAGAATCT TTCGGTGTGC CCAGAGGATG AATCAATCAT CATGTCCTCT
    TTTGTAAACA CTATGACTTC GCTAAGTGTG AAGCAGGTTG AGGATGGAGA GGTATTTGAC
    TTCAGAGGAA TGAGATTAGA TTGGTTTAGA TTGCAGGCCT ACACCAGTGT CTCTAAAGCT
    TCTCTTGGAC TGGCAGACCA CAGGGAGCTA GGCAAAATGA TGAACACAAT CATCTTCCAC
    ACAAAAATGG TAGATTCTTT GGTGGAGATG TTGGTTGAAA CCTCAGATCT TTCTATATTT
    TGTTTTTATA GTCGTGCTTT TGAGAAGATG TTTCAGCAGT GTTTGGAGCT TCCCTCTCAA
    TCAAGATATT CAATTGCATT CCCACTGCTT TGCACTCATT TTATGAGTTG CACCCACGAA
    CTGTGCCCAG AAGAGCGACA TCACATCGGA GATCGTAGTC TTTCCTTATG TAACATGTTT
    CTGGATGAAA TGGCTAAGCA AGCTCGAAAT CTTATCACAG ACATTTGCAC AGAACAATGT
    ACGCTGAGTG ATCAGTTGCT GCCAAAGCAT TGTGCCAAAA CCATCAGCCA AGCAGTGAAC
    AAAAAATCCA AAAAACAGAC AGGAAAGAAA GGAGAACCTG AAAGGGAGAA ACCAGGAGTA
    GAAAGCATGA GAAAAAACAG ATTGGTTGTG ACAAACCTGG ACAAACTGCA CACTGCACTT
    TCGGAGCTCT GCTTCTCAAT CAATTATGTA CCAAACATGG TGGTTTGGGA GCACACATTT
    ACCCCAAGAG AATATTTAAC GTCTCACCTG GAAATCCGCT TTACCAAGTC CATTGTTGGA
    ATGACGATGT ATAATCAAGC AACACAGGAG ATTGCAAAGC CTTCAGAGCT GTTAACCAGT
    GTAAGAGCCT ATATGACGGT CCTCCAGTCA ATAGAAAACT ATGTACAGAT TGACATTACA
    CGAGTATTTA ACAATGTTCT GCTTCAGCAA ACTCAGCATT TAGATAGTCA TGGTGAGCCA
    ACCATCACCA GTCTGTACAC AAATTGGTAT TTGGAAACAT TGCTAAGGCA AGTCAGCAAT
    GGTCACATAG CCTATTTTCC AGCTATGAAG GCATTTGTGA ATTTGCCAAC AGAAAATGAA
    TTAACATTTA ATGCTGAGGA GTACTCTGAC ATATCAGAAA TGAGAGCCCT TTCTGAACTC
    CTGGGACCAT ATGGCATGAA GTTCTTAAGC GAAAGTCTGA TGTGGCACAT TTCTTCGCAG
    GTTGCCGAGC TTAAGAAACT CGTGGTGGAG AATGTTGAAG TTCTAACACA AATGAGGACC
    AGCTTCGATA AGCCAGACCA GATGGCAGCA CTCTTCAAAA GATTATCATC TGTTGACAGT
    GTTTTGAAGA GAATGACGAT TATTGGTGTC ATCTTGTCTT TTCGGTCGTT GGCACAGGAA
    GCACTTAGAG ATGTCTTATC CTACCACATT CCTTTCCTTG TAAGTTCCAT TGAGGACTTC
    AAAGATCACA TTCCAAGAGA GACTGACATG AAGGTGGCAA TGAATGTATA TGAACTGTCA
    TCAGCTGCTG GATTGCCTTG CGAGATTGAT CCTGCCTTGG TTGTAGCACT GTCCTCCCAA
    AAATCAGAAA ACATCAGTCC AGAAGAAGAA TACAAAATTG CTTGCCTTCT CATGGTCTTC
    GTGGCAGTCT CCTTGCCAAC TTTGGCTAGT AACGTGATGT CTCAGTATAG CCCTGCCATT
    GAAGGGCACT GCAACAACAT TCATTGCTTG GCAAAAGCTA TCAACCAGAT TGCTGCAGCT
    TTATTTACCA TCCACAAGGG AAGCATCGAA GACCGTCTAA AAGAATTTTT GGCTCTTGCA
    TCATCTAGTC TACTGAAGAT CGGCCAGGAG ACGGACAAAA CTACTACAAG AAACAGAGAG
    TCTGTTTACC TACTACTGGA CATGATTGTG CAGGAGTCCC CGTTCCTGAC GATGGACCTC
    CTGGAGTCGT GCTTCCCCTA CGTCTTGCTG AGAAACGCGT ACCACGCTGT CTACAAACAA
    AGCGTCACCT CCTCTGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015144837.1
    CDS52..3432
    Translation

    Target ORF information:

    RefSeq Version XM_015289351.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus NCK-associated protein 1 (NCKAP1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289351.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCGCGGT CCGTGCTTCA GCCGAGCCAG CAGAAGCTGG CCGAGAAGCT GACCATCCTA 
    AACGACCGGG GCGTCGGCAT GCTCACCCGG CTCTACAACA TCAAGAAGGC ATGTGGAGAC
    CCCAAAGCCA AACCATCCTA TCTTATTGAC AAAAACCTGG AATCTGCAGT CAAATTCATA
    GTCAGGAAGT TTCCAGCGGT AGAAACACGC AACAACAATC TTGCTCAGCT ACAGAAAGAG
    AAGTCAGAGA TTTTGAAGAA CCTGGCGTTG TACTACTTCA CGTTTGTTGA TGTTATGGAA
    TTTAAGGACC ATGTTTGCGA GCTGCTGAAC ACTATTGATG TTTGCCAAGT CTTCTTTGAT
    ATTACTGTAA ACTTTGATTT GACGAAGAAC TACCTGGACT TGATTATAAC CTACACTACA
    CTAATGATAC TGCTGTCTCG AATTGAAGAA AGGAAGGCTA TCATTGGATT GTATAACTAT
    GCTCATGAAA TGACACATGG AGCGAGTGAC AGAGAATATC CACGCCTTGG CCAGATGATT
    GTGGATTATG AAAACCCTTT AAAGAAAATG ATGGAGGAAT TTGTACCCCA CAGTAAATCC
    CTTTCGGATG CTCTGATTTC ACTCCAGATG GTCTATCCTC GAAGAAATCT CTCGGCTGAC
    CAGTGGAGAA ACGCACAACT ACTGAGCCTC ATCAGTGCAC CTAGTACAAT GCTTAACCCT
    GCACAGTCTG ACACGATGCC ATGTGAATAC CTCTCTCTGG ATGCAATGGA AAAATGGATT
    ATTTTTGGAT TTATTTTGTG CCATGGAGTC CTAAATAGTG ATGCGACAGC TTTGAACCTT
    TGGAAACTTG CACTTCAAAG CAGCTCCTGC TTGGCTCTCT TTAGAGATGA AGTATTCCAT
    ATTCACAAAG CTGCAGAAGA CTTATTTGTA AACATACGAG GCTACAACAA GCGTATTAAT
    GACATCAGAG AGTGTAAAGA AGCTGCTGTT TCACATGCCG GTTCGATGCA CAGAGAGAGG
    CGCAAGTTTT TACGTTCTGC ACTCAAAGAA CTGGCGACAG TCCTGTCTGA TCAGCCAGGC
    CTGCTGGGCC CCAAGGCACT TTTTGTATTT ATGGCATTAT CCTTTGCTCG TGATGAAATC
    ATTTGGCTTC TTCGTCATGC AGATAACATG CCAAAGAAGA GTGCAGATGA CTTCATAGAT
    AAGCACATAG CTGAGCTAAT ATTCTACATG GAGGAGCTCA GAGCACATGT GCGAAAATAC
    GGACCTGTGA TGCAGAGATA CTACGTACAG TATCTGTCTG GCTTTGATGC AGTGGTCCTA
    AATGAACTTG TTCAGAATCT TTCGGTGTGC CCAGAGGATG AATCAATCAT CATGTCCTCT
    TTTGTAAACA CTATGACTTC GCTAAGTGTG AAGCAGGTTG AGGATGGAGA GGTATTTGAC
    TTCAGAGGAA TGAGATTAGA TTGGTTTAGA TTGCAGGCCT ACACCAGTGT CTCTAAAGCT
    TCTCTTGGAC TGGCAGACCA CAGGGAGCTA GGCAAAATGA TGAACACAAT CATCTTCCAC
    ACAAAAATGG TAGATTCTTT GGTGGAGATG TTGGTTGAAA CCTCAGATCT TTCTATATTT
    TGTTTTTATA GTCGTGCTTT TGAGAAGATG TTTCAGCAGT GTTTGGAGCT TCCCTCTCAA
    TCAAGATATT CAATTGCATT CCCACTGCTT TGCACTCATT TTATGAGTTG CACCCACGAA
    CTGTGCCCAG AAGAGCGACA TCACATCGGA GATCGTAGTC TTTCCTTATG TAACATGTTT
    CTGGATGAAA TGGCTAAGCA AGCTCGAAAT CTTATCACAG ACATTTGCAC AGAACAATGT
    ACGCTGAGTG ATCAGTTGCT GCCAAAGCAT TGTGCCAAAA CCATCAGCCA AGCAGTGAAC
    AAAAAATCCA AAAAACAGAC AGGAAAGAAA GGAGAACCTG AAAGGGAGAA ACCAGGAGTA
    GAAAGCATGA GAAAAAACAG ATTGGTTGTG ACAAACCTGG ACAAACTGCA CACTGCACTT
    TCGGAGCTCT GCTTCTCAAT CAATTATGTA CCAAACATGG TGGTTTGGGA GCACACATTT
    ACCCCAAGAG AATATTTAAC GTCTCACCTG GAAATCCGCT TTACCAAGTC CATTGTTGGA
    ATGACGATGT ATAATCAAGC AACACAGGAG ATTGCAAAGC CTTCAGAGCT GTTAACCAGT
    GTAAGAGCCT ATATGACGGT CCTCCAGTCA ATAGAAAACT ATGTACAGAT TGACATTACA
    CGAGTATTTA ACAATGTTCT GCTTCAGCAA ACTCAGCATT TAGATAGTCA TGGTGAGCCA
    ACCATCACCA GTCTGTACAC AAATTGGTAT TTGGAAACAT TGCTAAGGCA AGTCAGCAAT
    GGTCACATAG CCTATTTTCC AGCTATGAAG GCATTTGTGA ATTTGCCAAC AGAAAATGAA
    TTAACATTTA ATGCTGAGGA GTACTCTGAC ATATCAGAAA TGAGAGCCCT TTCTGAACTC
    CTGGGACCAT ATGGCATGAA GTTCTTAAGC GAAAGTCTGA TGTGGCACAT TTCTTCGCAG
    GTTGCCGAGC TTAAGAAACT CGTGGTGGAG AATGTTGAAG TTCTAACACA AATGAGGACC
    AGCTTCGATA AGCCAGACCA GATGGCAGCA CTCTTCAAAA GATTATCATC TGTTGACAGT
    GTTTTGAAGA GAATGACGAT TATTGGTGTC ATCTTGTCTT TTCGGTCGTT GGCACAGGAA
    GCACTTAGAG ATGTCTTATC CTACCACATT CCTTTCCTTG TAAGTTCCAT TGAGGACTTC
    AAAGATCACA TTCCAAGAGA GACTGACATG AAGGTGGCAA TGAATGTATA TGAACTGTCA
    TCAGCTGCTG GATTGCCTTG CGAGATTGAT CCTGCCTTGG TTGTAGCACT GTCCTCCCAA
    AAATCAGAAA ACATCAGTCC AGAAGAAGAA TACAAAATTG CTTGCCTTCT CATGGTCTTC
    GTGGCAGTCT CCTTGCCAAC TTTGGCTAGT AACGTGATGT CTCAGTATAG CCCTGCCATT
    GAAGGGCACT GCAACAACAT TCATTGCTTG GCAAAAGCTA TCAACCAGAT TGCTGCAGCT
    TTATTTACCA TCCACAAGGG AAGCATCGAA GACCGTCTAA AAGAATTTTT GGCTCTTGCA
    TCATCTAGTC TACTGAAGAT CGGCCAGGAG ACGGACAAAA CTACTACAAG AAACAGAGAG
    TCTGTTTACC TACTACTGGA CATGATTGTG CAGGAGTCCC CGTTCCTGAC GATGGACCTC
    CTGGAGTCGT GCTTCCCCTA CGTCTTGCTG AGAAACGCGT ACCACGCTGT CTACAAACAA
    AGCGTCACCT CCTCTGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa38284
    Clone ID Related Accession (Same CDS sequence) XM_015289351.1 , XM_015289351.2
    Accession Version XM_015289351.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3381bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product nck-associated protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289351.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCGCGGT CCGTGCTTCA GCCGAGCCAG CAGAAGCTGG CCGAGAAGCT GACCATCCTA 
    AACGACCGGG GCGTCGGCAT GCTCACCCGG CTCTACAACA TCAAGAAGGC ATGTGGAGAC
    CCCAAAGCCA AACCATCCTA TCTTATTGAC AAAAACCTGG AATCTGCAGT CAAATTCATA
    GTCAGGAAGT TTCCAGCGGT AGAAACACGC AACAACAATC TTGCTCAGCT ACAGAAAGAG
    AAGTCAGAGA TTTTGAAGAA CCTGGCGTTG TACTACTTCA CGTTTGTTGA TGTTATGGAA
    TTTAAGGACC ATGTTTGCGA GCTGCTGAAC ACTATTGATG TTTGCCAAGT CTTCTTTGAT
    ATTACTGTAA ACTTTGATTT GACGAAGAAC TACCTGGACT TGATTATAAC CTACACTACA
    CTAATGATAC TGCTGTCTCG AATTGAAGAA AGGAAGGCTA TCATTGGATT GTATAACTAT
    GCTCATGAAA TGACACATGG AGCGAGTGAC AGAGAATATC CACGCCTTGG CCAGATGATT
    GTGGATTATG AAAACCCTTT AAAGAAAATG ATGGAGGAAT TTGTACCCCA CAGTAAATCC
    CTTTCGGATG CTCTGATTTC ACTCCAGATG GTCTATCCTC GAAGAAATCT CTCGGCTGAC
    CAGTGGAGAA ACGCACAACT ACTGAGCCTC ATCAGTGCAC CTAGTACAAT GCTTAACCCT
    GCACAGTCTG ACACGATGCC ATGTGAATAC CTCTCTCTGG ATGCAATGGA AAAATGGATT
    ATTTTTGGAT TTATTTTGTG CCATGGAGTC CTAAATAGTG ATGCGACAGC TTTGAACCTT
    TGGAAACTTG CACTTCAAAG CAGCTCCTGC TTGGCTCTCT TTAGAGATGA AGTATTCCAT
    ATTCACAAAG CTGCAGAAGA CTTATTTGTA AACATACGAG GCTACAACAA GCGTATTAAT
    GACATCAGAG AGTGTAAAGA AGCTGCTGTT TCACATGCCG GTTCGATGCA CAGAGAGAGG
    CGCAAGTTTT TACGTTCTGC ACTCAAAGAA CTGGCGACAG TCCTGTCTGA TCAGCCAGGC
    CTGCTGGGCC CCAAGGCACT TTTTGTATTT ATGGCATTAT CCTTTGCTCG TGATGAAATC
    ATTTGGCTTC TTCGTCATGC AGATAACATG CCAAAGAAGA GTGCAGATGA CTTCATAGAT
    AAGCACATAG CTGAGCTAAT ATTCTACATG GAGGAGCTCA GAGCACATGT GCGAAAATAC
    GGACCTGTGA TGCAGAGATA CTACGTACAG TATCTGTCTG GCTTTGATGC AGTGGTCCTA
    AATGAACTTG TTCAGAATCT TTCGGTGTGC CCAGAGGATG AATCAATCAT CATGTCCTCT
    TTTGTAAACA CTATGACTTC GCTAAGTGTG AAGCAGGTTG AGGATGGAGA GGTATTTGAC
    TTCAGAGGAA TGAGATTAGA TTGGTTTAGA TTGCAGGCCT ACACCAGTGT CTCTAAAGCT
    TCTCTTGGAC TGGCAGACCA CAGGGAGCTA GGCAAAATGA TGAACACAAT CATCTTCCAC
    ACAAAAATGG TAGATTCTTT GGTGGAGATG TTGGTTGAAA CCTCAGATCT TTCTATATTT
    TGTTTTTATA GTCGTGCTTT TGAGAAGATG TTTCAGCAGT GTTTGGAGCT TCCCTCTCAA
    TCAAGATATT CAATTGCATT CCCACTGCTT TGCACTCATT TTATGAGTTG CACCCACGAA
    CTGTGCCCAG AAGAGCGACA TCACATCGGA GATCGTAGTC TTTCCTTATG TAACATGTTT
    CTGGATGAAA TGGCTAAGCA AGCTCGAAAT CTTATCACAG ACATTTGCAC AGAACAATGT
    ACGCTGAGTG ATCAGTTGCT GCCAAAGCAT TGTGCCAAAA CCATCAGCCA AGCAGTGAAC
    AAAAAATCCA AAAAACAGAC AGGAAAGAAA GGAGAACCTG AAAGGGAGAA ACCAGGAGTA
    GAAAGCATGA GAAAAAACAG ATTGGTTGTG ACAAACCTGG ACAAACTGCA CACTGCACTT
    TCGGAGCTCT GCTTCTCAAT CAATTATGTA CCAAACATGG TGGTTTGGGA GCACACATTT
    ACCCCAAGAG AATATTTAAC GTCTCACCTG GAAATCCGCT TTACCAAGTC CATTGTTGGA
    ATGACGATGT ATAATCAAGC AACACAGGAG ATTGCAAAGC CTTCAGAGCT GTTAACCAGT
    GTAAGAGCCT ATATGACGGT CCTCCAGTCA ATAGAAAACT ATGTACAGAT TGACATTACA
    CGAGTATTTA ACAATGTTCT GCTTCAGCAA ACTCAGCATT TAGATAGTCA TGGTGAGCCA
    ACCATCACCA GTCTGTACAC AAATTGGTAT TTGGAAACAT TGCTAAGGCA AGTCAGCAAT
    GGTCACATAG CCTATTTTCC AGCTATGAAG GCATTTGTGA ATTTGCCAAC AGAAAATGAA
    TTAACATTTA ATGCTGAGGA GTACTCTGAC ATATCAGAAA TGAGAGCCCT TTCTGAACTC
    CTGGGACCAT ATGGCATGAA GTTCTTAAGC GAAAGTCTGA TGTGGCACAT TTCTTCGCAG
    GTTGCCGAGC TTAAGAAACT CGTGGTGGAG AATGTTGAAG TTCTAACACA AATGAGGACC
    AGCTTCGATA AGCCAGACCA GATGGCAGCA CTCTTCAAAA GATTATCATC TGTTGACAGT
    GTTTTGAAGA GAATGACGAT TATTGGTGTC ATCTTGTCTT TTCGGTCGTT GGCACAGGAA
    GCACTTAGAG ATGTCTTATC CTACCACATT CCTTTCCTTG TAAGTTCCAT TGAGGACTTC
    AAAGATCACA TTCCAAGAGA GACTGACATG AAGGTGGCAA TGAATGTATA TGAACTGTCA
    TCAGCTGCTG GATTGCCTTG CGAGATTGAT CCTGCCTTGG TTGTAGCACT GTCCTCCCAA
    AAATCAGAAA ACATCAGTCC AGAAGAAGAA TACAAAATTG CTTGCCTTCT CATGGTCTTC
    GTGGCAGTCT CCTTGCCAAC TTTGGCTAGT AACGTGATGT CTCAGTATAG CCCTGCCATT
    GAAGGGCACT GCAACAACAT TCATTGCTTG GCAAAAGCTA TCAACCAGAT TGCTGCAGCT
    TTATTTACCA TCCACAAGGG AAGCATCGAA GACCGTCTAA AAGAATTTTT GGCTCTTGCA
    TCATCTAGTC TACTGAAGAT CGGCCAGGAG ACGGACAAAA CTACTACAAG AAACAGAGAG
    TCTGTTTACC TACTACTGGA CATGATTGTG CAGGAGTCCC CGTTCCTGAC GATGGACCTC
    CTGGAGTCGT GCTTCCCCTA CGTCTTGCTG AGAAACGCGT ACCACGCTGT CTACAAACAA
    AGCGTCACCT CCTCTGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015144837.1
    CDS64..3444
    Translation

    Target ORF information:

    RefSeq Version XM_015289351.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus NCK associated protein 1 (NCKAP1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289351.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCGCGGT CCGTGCTTCA GCCGAGCCAG CAGAAGCTGG CCGAGAAGCT GACCATCCTA 
    AACGACCGGG GCGTCGGCAT GCTCACCCGG CTCTACAACA TCAAGAAGGC ATGTGGAGAC
    CCCAAAGCCA AACCATCCTA TCTTATTGAC AAAAACCTGG AATCTGCAGT CAAATTCATA
    GTCAGGAAGT TTCCAGCGGT AGAAACACGC AACAACAATC TTGCTCAGCT ACAGAAAGAG
    AAGTCAGAGA TTTTGAAGAA CCTGGCGTTG TACTACTTCA CGTTTGTTGA TGTTATGGAA
    TTTAAGGACC ATGTTTGCGA GCTGCTGAAC ACTATTGATG TTTGCCAAGT CTTCTTTGAT
    ATTACTGTAA ACTTTGATTT GACGAAGAAC TACCTGGACT TGATTATAAC CTACACTACA
    CTAATGATAC TGCTGTCTCG AATTGAAGAA AGGAAGGCTA TCATTGGATT GTATAACTAT
    GCTCATGAAA TGACACATGG AGCGAGTGAC AGAGAATATC CACGCCTTGG CCAGATGATT
    GTGGATTATG AAAACCCTTT AAAGAAAATG ATGGAGGAAT TTGTACCCCA CAGTAAATCC
    CTTTCGGATG CTCTGATTTC ACTCCAGATG GTCTATCCTC GAAGAAATCT CTCGGCTGAC
    CAGTGGAGAA ACGCACAACT ACTGAGCCTC ATCAGTGCAC CTAGTACAAT GCTTAACCCT
    GCACAGTCTG ACACGATGCC ATGTGAATAC CTCTCTCTGG ATGCAATGGA AAAATGGATT
    ATTTTTGGAT TTATTTTGTG CCATGGAGTC CTAAATAGTG ATGCGACAGC TTTGAACCTT
    TGGAAACTTG CACTTCAAAG CAGCTCCTGC TTGGCTCTCT TTAGAGATGA AGTATTCCAT
    ATTCACAAAG CTGCAGAAGA CTTATTTGTA AACATACGAG GCTACAACAA GCGTATTAAT
    GACATCAGAG AGTGTAAAGA AGCTGCTGTT TCACATGCCG GTTCGATGCA CAGAGAGAGG
    CGCAAGTTTT TACGTTCTGC ACTCAAAGAA CTGGCGACAG TCCTGTCTGA TCAGCCAGGC
    CTGCTGGGCC CCAAGGCACT TTTTGTATTT ATGGCATTAT CCTTTGCTCG TGATGAAATC
    ATTTGGCTTC TTCGTCATGC AGATAACATG CCAAAGAAGA GTGCAGATGA CTTCATAGAT
    AAGCACATAG CTGAGCTAAT ATTCTACATG GAGGAGCTCA GAGCACATGT GCGAAAATAC
    GGACCTGTGA TGCAGAGATA CTACGTACAG TATCTGTCTG GCTTTGATGC AGTGGTCCTA
    AATGAACTTG TTCAGAATCT TTCGGTGTGC CCAGAGGATG AATCAATCAT CATGTCCTCT
    TTTGTAAACA CTATGACTTC GCTAAGTGTG AAGCAGGTTG AGGATGGAGA GGTATTTGAC
    TTCAGAGGAA TGAGATTAGA TTGGTTTAGA TTGCAGGCCT ACACCAGTGT CTCTAAAGCT
    TCTCTTGGAC TGGCAGACCA CAGGGAGCTA GGCAAAATGA TGAACACAAT CATCTTCCAC
    ACAAAAATGG TAGATTCTTT GGTGGAGATG TTGGTTGAAA CCTCAGATCT TTCTATATTT
    TGTTTTTATA GTCGTGCTTT TGAGAAGATG TTTCAGCAGT GTTTGGAGCT TCCCTCTCAA
    TCAAGATATT CAATTGCATT CCCACTGCTT TGCACTCATT TTATGAGTTG CACCCACGAA
    CTGTGCCCAG AAGAGCGACA TCACATCGGA GATCGTAGTC TTTCCTTATG TAACATGTTT
    CTGGATGAAA TGGCTAAGCA AGCTCGAAAT CTTATCACAG ACATTTGCAC AGAACAATGT
    ACGCTGAGTG ATCAGTTGCT GCCAAAGCAT TGTGCCAAAA CCATCAGCCA AGCAGTGAAC
    AAAAAATCCA AAAAACAGAC AGGAAAGAAA GGAGAACCTG AAAGGGAGAA ACCAGGAGTA
    GAAAGCATGA GAAAAAACAG ATTGGTTGTG ACAAACCTGG ACAAACTGCA CACTGCACTT
    TCGGAGCTCT GCTTCTCAAT CAATTATGTA CCAAACATGG TGGTTTGGGA GCACACATTT
    ACCCCAAGAG AATATTTAAC GTCTCACCTG GAAATCCGCT TTACCAAGTC CATTGTTGGA
    ATGACGATGT ATAATCAAGC AACACAGGAG ATTGCAAAGC CTTCAGAGCT GTTAACCAGT
    GTAAGAGCCT ATATGACGGT CCTCCAGTCA ATAGAAAACT ATGTACAGAT TGACATTACA
    CGAGTATTTA ACAATGTTCT GCTTCAGCAA ACTCAGCATT TAGATAGTCA TGGTGAGCCA
    ACCATCACCA GTCTGTACAC AAATTGGTAT TTGGAAACAT TGCTAAGGCA AGTCAGCAAT
    GGTCACATAG CCTATTTTCC AGCTATGAAG GCATTTGTGA ATTTGCCAAC AGAAAATGAA
    TTAACATTTA ATGCTGAGGA GTACTCTGAC ATATCAGAAA TGAGAGCCCT TTCTGAACTC
    CTGGGACCAT ATGGCATGAA GTTCTTAAGC GAAAGTCTGA TGTGGCACAT TTCTTCGCAG
    GTTGCCGAGC TTAAGAAACT CGTGGTGGAG AATGTTGAAG TTCTAACACA AATGAGGACC
    AGCTTCGATA AGCCAGACCA GATGGCAGCA CTCTTCAAAA GATTATCATC TGTTGACAGT
    GTTTTGAAGA GAATGACGAT TATTGGTGTC ATCTTGTCTT TTCGGTCGTT GGCACAGGAA
    GCACTTAGAG ATGTCTTATC CTACCACATT CCTTTCCTTG TAAGTTCCAT TGAGGACTTC
    AAAGATCACA TTCCAAGAGA GACTGACATG AAGGTGGCAA TGAATGTATA TGAACTGTCA
    TCAGCTGCTG GATTGCCTTG CGAGATTGAT CCTGCCTTGG TTGTAGCACT GTCCTCCCAA
    AAATCAGAAA ACATCAGTCC AGAAGAAGAA TACAAAATTG CTTGCCTTCT CATGGTCTTC
    GTGGCAGTCT CCTTGCCAAC TTTGGCTAGT AACGTGATGT CTCAGTATAG CCCTGCCATT
    GAAGGGCACT GCAACAACAT TCATTGCTTG GCAAAAGCTA TCAACCAGAT TGCTGCAGCT
    TTATTTACCA TCCACAAGGG AAGCATCGAA GACCGTCTAA AAGAATTTTT GGCTCTTGCA
    TCATCTAGTC TACTGAAGAT CGGCCAGGAG ACGGACAAAA CTACTACAAG AAACAGAGAG
    TCTGTTTACC TACTACTGGA CATGATTGTG CAGGAGTCCC CGTTCCTGAC GATGGACCTC
    CTGGAGTCGT GCTTCCCCTA CGTCTTGCTG AGAAACGCGT ACCACGCTGT CTACAAACAA
    AGCGTCACCT CCTCTGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa10694
    Clone ID Related Accession (Same CDS sequence) XM_004942427.2 , XM_004942427.3
    Accession Version XM_004942427.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3195bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product nck-associated protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455901.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004942427.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGAAGG AAACACACAA AAACTTGCCA AAGCTTGCTC AGCTACAGAA AGAGAAGTCA 
    GAGATTTTGA AGAACCTGGC GTTGTACTAC TTCACGTTTG TTGATGTTAT GGAATTTAAG
    GACCATGTTT GCGAGCTGCT GAACACTATT GATGTTTGCC AAGTCTTCTT TGATATTACT
    GTAAACTTTG ATTTGACGAA GAACTACCTG GACTTGATTA TAACCTACAC TACACTAATG
    ATACTGCTGT CTCGAATTGA AGAAAGGAAG GCTATCATTG GATTGTATAA CTATGCTCAT
    GAAATGACAC ATGGAGCGAG TGACAGAGAA TATCCACGCC TTGGCCAGAT GATTGTGGAT
    TATGAAAACC CTTTAAAGAA AATGATGGAG GAATTTGTAC CCCACAGTAA ATCCCTTTCG
    GATGCTCTGA TTTCACTCCA GATGGTCTAT CCTCGAAGAA ATCTCTCGGC TGACCAGTGG
    AGAAACGCAC AACTACTGAG CCTCATCAGT GCACCTAGTA CAATGCTTAA CCCTGCACAG
    TCTGACACGA TGCCATGTGA ATACCTCTCT CTGGATGCAA TGGAAAAATG GATTATTTTT
    GGATTTATTT TGTGCCATGG AGTCCTAAAT AGTGATGCGA CAGCTTTGAA CCTTTGGAAA
    CTTGCACTTC AAAGCAGCTC CTGCTTGGCT CTCTTTAGAG ATGAAGTATT CCATATTCAC
    AAAGCTGCAG AAGACTTATT TGTAAACATA CGAGGCTACA ACAAGCGTAT TAATGACATC
    AGAGAGTGTA AAGAAGCTGC TGTTTCACAT GCCGGTTCGA TGCACAGAGA GAGGCGCAAG
    TTTTTACGTT CTGCACTCAA AGAACTGGCG ACAGTCCTGT CTGATCAGCC AGGCCTGCTG
    GGCCCCAAGG CACTTTTTGT ATTTATGGCA TTATCCTTTG CTCGTGATGA AATCATTTGG
    CTTCTTCGTC ATGCAGATAA CATGCCAAAG AAGAGTGCAG ATGACTTCAT AGATAAGCAC
    ATAGCTGAGC TAATATTCTA CATGGAGGAG CTCAGAGCAC ATGTGCGAAA ATACGGACCT
    GTGATGCAGA GATACTACGT ACAGTATCTG TCTGGCTTTG ATGCAGTGGT CCTAAATGAA
    CTTGTTCAGA ATCTTTCGGT GTGCCCAGAG GATGAATCAA TCATCATGTC CTCTTTTGTA
    AACACTATGA CTTCGCTAAG TGTGAAGCAG GTTGAGGATG GAGAGGTATT TGACTTCAGA
    GGAATGAGAT TAGATTGGTT TAGATTGCAG GCCTACACCA GTGTCTCTAA AGCTTCTCTT
    GGACTGGCAG ACCACAGGGA GCTAGGCAAA ATGATGAACA CAATCATCTT CCACACAAAA
    ATGGTAGATT CTTTGGTGGA GATGTTGGTT GAAACCTCAG ATCTTTCTAT ATTTTGTTTT
    TATAGTCGTG CTTTTGAGAA GATGTTTCAG CAGTGTTTGG AGCTTCCCTC TCAATCAAGA
    TATTCAATTG CATTCCCACT GCTTTGCACT CATTTTATGA GTTGCACCCA CGAACTGTGC
    CCAGAAGAGC GACATCACAT CGGAGATCGT AGTCTTTCCT TATGTAACAT GTTTCTGGAT
    GAAATGGCTA AGCAAGCTCG AAATCTTATC ACAGACATTT GCACAGAACA ATGTACGCTG
    AGTGATCAGT TGCTGCCAAA GCATTGTGCC AAAACCATCA GCCAAGCAGT GAACAAAAAA
    TCCAAAAAAC AGACAGGAAA GAAAGGAGAA CCTGAAAGGG AGAAACCAGG AGTAGAAAGC
    ATGAGAAAAA ACAGATTGGT TGTGACAAAC CTGGACAAAC TGCACACTGC ACTTTCGGAG
    CTCTGCTTCT CAATCAATTA TGTACCAAAC ATGGTGGTTT GGGAGCACAC ATTTACCCCA
    AGAGAATATT TAACGTCTCA CCTGGAAATC CGCTTTACCA AGTCCATTGT TGGAATGACG
    ATGTATAATC AAGCAACACA GGAGATTGCA AAGCCTTCAG AGCTGTTAAC CAGTGTAAGA
    GCCTATATGA CGGTCCTCCA GTCAATAGAA AACTATGTAC AGATTGACAT TACACGAGTA
    TTTAACAATG TTCTGCTTCA GCAAACTCAG CATTTAGATA GTCATGGTGA GCCAACCATC
    ACCAGTCTGT ACACAAATTG GTATTTGGAA ACATTGCTAA GGCAAGTCAG CAATGGTCAC
    ATAGCCTATT TTCCAGCTAT GAAGGCATTT GTGAATTTGC CAACAGAAAA TGAATTAACA
    TTTAATGCTG AGGAGTACTC TGACATATCA GAAATGAGAG CCCTTTCTGA ACTCCTGGGA
    CCATATGGCA TGAAGTTCTT AAGCGAAAGT CTGATGTGGC ACATTTCTTC GCAGGTTGCC
    GAGCTTAAGA AACTCGTGGT GGAGAATGTT GAAGTTCTAA CACAAATGAG GACCAGCTTC
    GATAAGCCAG ACCAGATGGC AGCACTCTTC AAAAGATTAT CATCTGTTGA CAGTGTTTTG
    AAGAGAATGA CGATTATTGG TGTCATCTTG TCTTTTCGGT CGTTGGCACA GGAAGCACTT
    AGAGATGTCT TATCCTACCA CATTCCTTTC CTTGTAAGTT CCATTGAGGA CTTCAAAGAT
    CACATTCCAA GAGAGACTGA CATGAAGGTG GCAATGAATG TATATGAACT GTCATCAGCT
    GCTGGATTGC CTTGCGAGAT TGATCCTGCC TTGGTTGTAG CACTGTCCTC CCAAAAATCA
    GAAAACATCA GTCCAGAAGA AGAATACAAA ATTGCTTGCC TTCTCATGGT CTTCGTGGCA
    GTCTCCTTGC CAACTTTGGC TAGTAACGTG ATGTCTCAGT ATAGCCCTGC CATTGAAGGG
    CACTGCAACA ACATTCATTG CTTGGCAAAA GCTATCAACC AGATTGCTGC AGCTTTATTT
    ACCATCCACA AGGGAAGCAT CGAAGACCGT CTAAAAGAAT TTTTGGCTCT TGCATCATCT
    AGTCTACTGA AGATCGGCCA GGAGACGGAC AAAACTACTA CAAGAAACAG AGAGTCTGTT
    TACCTACTAC TGGACATGAT TGTGCAGGAG TCCCCGTTCC TGACGATGGA CCTCCTGGAG
    TCGTGCTTCC CCTACGTCTT GCTGAGAAAC GCGTACCACG CTGTCTACAA ACAAAGCGTC
    ACCTCCTCTG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004942484.1
    CDS16..3210
    Translation

    Target ORF information:

    RefSeq Version XM_004942427.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus NCK-associated protein 1 (NCKAP1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004942427.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGAAGG AAACACACAA AAACTTGCCA AAGCTTGCTC AGCTACAGAA AGAGAAGTCA 
    GAGATTTTGA AGAACCTGGC GTTGTACTAC TTCACGTTTG TTGATGTTAT GGAATTTAAG
    GACCATGTTT GCGAGCTGCT GAACACTATT GATGTTTGCC AAGTCTTCTT TGATATTACT
    GTAAACTTTG ATTTGACGAA GAACTACCTG GACTTGATTA TAACCTACAC TACACTAATG
    ATACTGCTGT CTCGAATTGA AGAAAGGAAG GCTATCATTG GATTGTATAA CTATGCTCAT
    GAAATGACAC ATGGAGCGAG TGACAGAGAA TATCCACGCC TTGGCCAGAT GATTGTGGAT
    TATGAAAACC CTTTAAAGAA AATGATGGAG GAATTTGTAC CCCACAGTAA ATCCCTTTCG
    GATGCTCTGA TTTCACTCCA GATGGTCTAT CCTCGAAGAA ATCTCTCGGC TGACCAGTGG
    AGAAACGCAC AACTACTGAG CCTCATCAGT GCACCTAGTA CAATGCTTAA CCCTGCACAG
    TCTGACACGA TGCCATGTGA ATACCTCTCT CTGGATGCAA TGGAAAAATG GATTATTTTT
    GGATTTATTT TGTGCCATGG AGTCCTAAAT AGTGATGCGA CAGCTTTGAA CCTTTGGAAA
    CTTGCACTTC AAAGCAGCTC CTGCTTGGCT CTCTTTAGAG ATGAAGTATT CCATATTCAC
    AAAGCTGCAG AAGACTTATT TGTAAACATA CGAGGCTACA ACAAGCGTAT TAATGACATC
    AGAGAGTGTA AAGAAGCTGC TGTTTCACAT GCCGGTTCGA TGCACAGAGA GAGGCGCAAG
    TTTTTACGTT CTGCACTCAA AGAACTGGCG ACAGTCCTGT CTGATCAGCC AGGCCTGCTG
    GGCCCCAAGG CACTTTTTGT ATTTATGGCA TTATCCTTTG CTCGTGATGA AATCATTTGG
    CTTCTTCGTC ATGCAGATAA CATGCCAAAG AAGAGTGCAG ATGACTTCAT AGATAAGCAC
    ATAGCTGAGC TAATATTCTA CATGGAGGAG CTCAGAGCAC ATGTGCGAAA ATACGGACCT
    GTGATGCAGA GATACTACGT ACAGTATCTG TCTGGCTTTG ATGCAGTGGT CCTAAATGAA
    CTTGTTCAGA ATCTTTCGGT GTGCCCAGAG GATGAATCAA TCATCATGTC CTCTTTTGTA
    AACACTATGA CTTCGCTAAG TGTGAAGCAG GTTGAGGATG GAGAGGTATT TGACTTCAGA
    GGAATGAGAT TAGATTGGTT TAGATTGCAG GCCTACACCA GTGTCTCTAA AGCTTCTCTT
    GGACTGGCAG ACCACAGGGA GCTAGGCAAA ATGATGAACA CAATCATCTT CCACACAAAA
    ATGGTAGATT CTTTGGTGGA GATGTTGGTT GAAACCTCAG ATCTTTCTAT ATTTTGTTTT
    TATAGTCGTG CTTTTGAGAA GATGTTTCAG CAGTGTTTGG AGCTTCCCTC TCAATCAAGA
    TATTCAATTG CATTCCCACT GCTTTGCACT CATTTTATGA GTTGCACCCA CGAACTGTGC
    CCAGAAGAGC GACATCACAT CGGAGATCGT AGTCTTTCCT TATGTAACAT GTTTCTGGAT
    GAAATGGCTA AGCAAGCTCG AAATCTTATC ACAGACATTT GCACAGAACA ATGTACGCTG
    AGTGATCAGT TGCTGCCAAA GCATTGTGCC AAAACCATCA GCCAAGCAGT GAACAAAAAA
    TCCAAAAAAC AGACAGGAAA GAAAGGAGAA CCTGAAAGGG AGAAACCAGG AGTAGAAAGC
    ATGAGAAAAA ACAGATTGGT TGTGACAAAC CTGGACAAAC TGCACACTGC ACTTTCGGAG
    CTCTGCTTCT CAATCAATTA TGTACCAAAC ATGGTGGTTT GGGAGCACAC ATTTACCCCA
    AGAGAATATT TAACGTCTCA CCTGGAAATC CGCTTTACCA AGTCCATTGT TGGAATGACG
    ATGTATAATC AAGCAACACA GGAGATTGCA AAGCCTTCAG AGCTGTTAAC CAGTGTAAGA
    GCCTATATGA CGGTCCTCCA GTCAATAGAA AACTATGTAC AGATTGACAT TACACGAGTA
    TTTAACAATG TTCTGCTTCA GCAAACTCAG CATTTAGATA GTCATGGTGA GCCAACCATC
    ACCAGTCTGT ACACAAATTG GTATTTGGAA ACATTGCTAA GGCAAGTCAG CAATGGTCAC
    ATAGCCTATT TTCCAGCTAT GAAGGCATTT GTGAATTTGC CAACAGAAAA TGAATTAACA
    TTTAATGCTG AGGAGTACTC TGACATATCA GAAATGAGAG CCCTTTCTGA ACTCCTGGGA
    CCATATGGCA TGAAGTTCTT AAGCGAAAGT CTGATGTGGC ACATTTCTTC GCAGGTTGCC
    GAGCTTAAGA AACTCGTGGT GGAGAATGTT GAAGTTCTAA CACAAATGAG GACCAGCTTC
    GATAAGCCAG ACCAGATGGC AGCACTCTTC AAAAGATTAT CATCTGTTGA CAGTGTTTTG
    AAGAGAATGA CGATTATTGG TGTCATCTTG TCTTTTCGGT CGTTGGCACA GGAAGCACTT
    AGAGATGTCT TATCCTACCA CATTCCTTTC CTTGTAAGTT CCATTGAGGA CTTCAAAGAT
    CACATTCCAA GAGAGACTGA CATGAAGGTG GCAATGAATG TATATGAACT GTCATCAGCT
    GCTGGATTGC CTTGCGAGAT TGATCCTGCC TTGGTTGTAG CACTGTCCTC CCAAAAATCA
    GAAAACATCA GTCCAGAAGA AGAATACAAA ATTGCTTGCC TTCTCATGGT CTTCGTGGCA
    GTCTCCTTGC CAACTTTGGC TAGTAACGTG ATGTCTCAGT ATAGCCCTGC CATTGAAGGG
    CACTGCAACA ACATTCATTG CTTGGCAAAA GCTATCAACC AGATTGCTGC AGCTTTATTT
    ACCATCCACA AGGGAAGCAT CGAAGACCGT CTAAAAGAAT TTTTGGCTCT TGCATCATCT
    AGTCTACTGA AGATCGGCCA GGAGACGGAC AAAACTACTA CAAGAAACAG AGAGTCTGTT
    TACCTACTAC TGGACATGAT TGTGCAGGAG TCCCCGTTCC TGACGATGGA CCTCCTGGAG
    TCGTGCTTCC CCTACGTCTT GCTGAGAAAC GCGTACCACG CTGTCTACAA ACAAAGCGTC
    ACCTCCTCTG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa10694
    Clone ID Related Accession (Same CDS sequence) XM_004942427.2 , XM_004942427.3
    Accession Version XM_004942427.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3195bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product nck-associated protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004942427.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGAAGG AAACACACAA AAACTTGCCA AAGCTTGCTC AGCTACAGAA AGAGAAGTCA 
    GAGATTTTGA AGAACCTGGC GTTGTACTAC TTCACGTTTG TTGATGTTAT GGAATTTAAG
    GACCATGTTT GCGAGCTGCT GAACACTATT GATGTTTGCC AAGTCTTCTT TGATATTACT
    GTAAACTTTG ATTTGACGAA GAACTACCTG GACTTGATTA TAACCTACAC TACACTAATG
    ATACTGCTGT CTCGAATTGA AGAAAGGAAG GCTATCATTG GATTGTATAA CTATGCTCAT
    GAAATGACAC ATGGAGCGAG TGACAGAGAA TATCCACGCC TTGGCCAGAT GATTGTGGAT
    TATGAAAACC CTTTAAAGAA AATGATGGAG GAATTTGTAC CCCACAGTAA ATCCCTTTCG
    GATGCTCTGA TTTCACTCCA GATGGTCTAT CCTCGAAGAA ATCTCTCGGC TGACCAGTGG
    AGAAACGCAC AACTACTGAG CCTCATCAGT GCACCTAGTA CAATGCTTAA CCCTGCACAG
    TCTGACACGA TGCCATGTGA ATACCTCTCT CTGGATGCAA TGGAAAAATG GATTATTTTT
    GGATTTATTT TGTGCCATGG AGTCCTAAAT AGTGATGCGA CAGCTTTGAA CCTTTGGAAA
    CTTGCACTTC AAAGCAGCTC CTGCTTGGCT CTCTTTAGAG ATGAAGTATT CCATATTCAC
    AAAGCTGCAG AAGACTTATT TGTAAACATA CGAGGCTACA ACAAGCGTAT TAATGACATC
    AGAGAGTGTA AAGAAGCTGC TGTTTCACAT GCCGGTTCGA TGCACAGAGA GAGGCGCAAG
    TTTTTACGTT CTGCACTCAA AGAACTGGCG ACAGTCCTGT CTGATCAGCC AGGCCTGCTG
    GGCCCCAAGG CACTTTTTGT ATTTATGGCA TTATCCTTTG CTCGTGATGA AATCATTTGG
    CTTCTTCGTC ATGCAGATAA CATGCCAAAG AAGAGTGCAG ATGACTTCAT AGATAAGCAC
    ATAGCTGAGC TAATATTCTA CATGGAGGAG CTCAGAGCAC ATGTGCGAAA ATACGGACCT
    GTGATGCAGA GATACTACGT ACAGTATCTG TCTGGCTTTG ATGCAGTGGT CCTAAATGAA
    CTTGTTCAGA ATCTTTCGGT GTGCCCAGAG GATGAATCAA TCATCATGTC CTCTTTTGTA
    AACACTATGA CTTCGCTAAG TGTGAAGCAG GTTGAGGATG GAGAGGTATT TGACTTCAGA
    GGAATGAGAT TAGATTGGTT TAGATTGCAG GCCTACACCA GTGTCTCTAA AGCTTCTCTT
    GGACTGGCAG ACCACAGGGA GCTAGGCAAA ATGATGAACA CAATCATCTT CCACACAAAA
    ATGGTAGATT CTTTGGTGGA GATGTTGGTT GAAACCTCAG ATCTTTCTAT ATTTTGTTTT
    TATAGTCGTG CTTTTGAGAA GATGTTTCAG CAGTGTTTGG AGCTTCCCTC TCAATCAAGA
    TATTCAATTG CATTCCCACT GCTTTGCACT CATTTTATGA GTTGCACCCA CGAACTGTGC
    CCAGAAGAGC GACATCACAT CGGAGATCGT AGTCTTTCCT TATGTAACAT GTTTCTGGAT
    GAAATGGCTA AGCAAGCTCG AAATCTTATC ACAGACATTT GCACAGAACA ATGTACGCTG
    AGTGATCAGT TGCTGCCAAA GCATTGTGCC AAAACCATCA GCCAAGCAGT GAACAAAAAA
    TCCAAAAAAC AGACAGGAAA GAAAGGAGAA CCTGAAAGGG AGAAACCAGG AGTAGAAAGC
    ATGAGAAAAA ACAGATTGGT TGTGACAAAC CTGGACAAAC TGCACACTGC ACTTTCGGAG
    CTCTGCTTCT CAATCAATTA TGTACCAAAC ATGGTGGTTT GGGAGCACAC ATTTACCCCA
    AGAGAATATT TAACGTCTCA CCTGGAAATC CGCTTTACCA AGTCCATTGT TGGAATGACG
    ATGTATAATC AAGCAACACA GGAGATTGCA AAGCCTTCAG AGCTGTTAAC CAGTGTAAGA
    GCCTATATGA CGGTCCTCCA GTCAATAGAA AACTATGTAC AGATTGACAT TACACGAGTA
    TTTAACAATG TTCTGCTTCA GCAAACTCAG CATTTAGATA GTCATGGTGA GCCAACCATC
    ACCAGTCTGT ACACAAATTG GTATTTGGAA ACATTGCTAA GGCAAGTCAG CAATGGTCAC
    ATAGCCTATT TTCCAGCTAT GAAGGCATTT GTGAATTTGC CAACAGAAAA TGAATTAACA
    TTTAATGCTG AGGAGTACTC TGACATATCA GAAATGAGAG CCCTTTCTGA ACTCCTGGGA
    CCATATGGCA TGAAGTTCTT AAGCGAAAGT CTGATGTGGC ACATTTCTTC GCAGGTTGCC
    GAGCTTAAGA AACTCGTGGT GGAGAATGTT GAAGTTCTAA CACAAATGAG GACCAGCTTC
    GATAAGCCAG ACCAGATGGC AGCACTCTTC AAAAGATTAT CATCTGTTGA CAGTGTTTTG
    AAGAGAATGA CGATTATTGG TGTCATCTTG TCTTTTCGGT CGTTGGCACA GGAAGCACTT
    AGAGATGTCT TATCCTACCA CATTCCTTTC CTTGTAAGTT CCATTGAGGA CTTCAAAGAT
    CACATTCCAA GAGAGACTGA CATGAAGGTG GCAATGAATG TATATGAACT GTCATCAGCT
    GCTGGATTGC CTTGCGAGAT TGATCCTGCC TTGGTTGTAG CACTGTCCTC CCAAAAATCA
    GAAAACATCA GTCCAGAAGA AGAATACAAA ATTGCTTGCC TTCTCATGGT CTTCGTGGCA
    GTCTCCTTGC CAACTTTGGC TAGTAACGTG ATGTCTCAGT ATAGCCCTGC CATTGAAGGG
    CACTGCAACA ACATTCATTG CTTGGCAAAA GCTATCAACC AGATTGCTGC AGCTTTATTT
    ACCATCCACA AGGGAAGCAT CGAAGACCGT CTAAAAGAAT TTTTGGCTCT TGCATCATCT
    AGTCTACTGA AGATCGGCCA GGAGACGGAC AAAACTACTA CAAGAAACAG AGAGTCTGTT
    TACCTACTAC TGGACATGAT TGTGCAGGAG TCCCCGTTCC TGACGATGGA CCTCCTGGAG
    TCGTGCTTCC CCTACGTCTT GCTGAGAAAC GCGTACCACG CTGTCTACAA ACAAAGCGTC
    ACCTCCTCTG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004942484.1
    CDS16..3210
    Translation

    Target ORF information:

    RefSeq Version XM_004942427.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus NCK associated protein 1 (NCKAP1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004942427.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGAAGG AAACACACAA AAACTTGCCA AAGCTTGCTC AGCTACAGAA AGAGAAGTCA 
    GAGATTTTGA AGAACCTGGC GTTGTACTAC TTCACGTTTG TTGATGTTAT GGAATTTAAG
    GACCATGTTT GCGAGCTGCT GAACACTATT GATGTTTGCC AAGTCTTCTT TGATATTACT
    GTAAACTTTG ATTTGACGAA GAACTACCTG GACTTGATTA TAACCTACAC TACACTAATG
    ATACTGCTGT CTCGAATTGA AGAAAGGAAG GCTATCATTG GATTGTATAA CTATGCTCAT
    GAAATGACAC ATGGAGCGAG TGACAGAGAA TATCCACGCC TTGGCCAGAT GATTGTGGAT
    TATGAAAACC CTTTAAAGAA AATGATGGAG GAATTTGTAC CCCACAGTAA ATCCCTTTCG
    GATGCTCTGA TTTCACTCCA GATGGTCTAT CCTCGAAGAA ATCTCTCGGC TGACCAGTGG
    AGAAACGCAC AACTACTGAG CCTCATCAGT GCACCTAGTA CAATGCTTAA CCCTGCACAG
    TCTGACACGA TGCCATGTGA ATACCTCTCT CTGGATGCAA TGGAAAAATG GATTATTTTT
    GGATTTATTT TGTGCCATGG AGTCCTAAAT AGTGATGCGA CAGCTTTGAA CCTTTGGAAA
    CTTGCACTTC AAAGCAGCTC CTGCTTGGCT CTCTTTAGAG ATGAAGTATT CCATATTCAC
    AAAGCTGCAG AAGACTTATT TGTAAACATA CGAGGCTACA ACAAGCGTAT TAATGACATC
    AGAGAGTGTA AAGAAGCTGC TGTTTCACAT GCCGGTTCGA TGCACAGAGA GAGGCGCAAG
    TTTTTACGTT CTGCACTCAA AGAACTGGCG ACAGTCCTGT CTGATCAGCC AGGCCTGCTG
    GGCCCCAAGG CACTTTTTGT ATTTATGGCA TTATCCTTTG CTCGTGATGA AATCATTTGG
    CTTCTTCGTC ATGCAGATAA CATGCCAAAG AAGAGTGCAG ATGACTTCAT AGATAAGCAC
    ATAGCTGAGC TAATATTCTA CATGGAGGAG CTCAGAGCAC ATGTGCGAAA ATACGGACCT
    GTGATGCAGA GATACTACGT ACAGTATCTG TCTGGCTTTG ATGCAGTGGT CCTAAATGAA
    CTTGTTCAGA ATCTTTCGGT GTGCCCAGAG GATGAATCAA TCATCATGTC CTCTTTTGTA
    AACACTATGA CTTCGCTAAG TGTGAAGCAG GTTGAGGATG GAGAGGTATT TGACTTCAGA
    GGAATGAGAT TAGATTGGTT TAGATTGCAG GCCTACACCA GTGTCTCTAA AGCTTCTCTT
    GGACTGGCAG ACCACAGGGA GCTAGGCAAA ATGATGAACA CAATCATCTT CCACACAAAA
    ATGGTAGATT CTTTGGTGGA GATGTTGGTT GAAACCTCAG ATCTTTCTAT ATTTTGTTTT
    TATAGTCGTG CTTTTGAGAA GATGTTTCAG CAGTGTTTGG AGCTTCCCTC TCAATCAAGA
    TATTCAATTG CATTCCCACT GCTTTGCACT CATTTTATGA GTTGCACCCA CGAACTGTGC
    CCAGAAGAGC GACATCACAT CGGAGATCGT AGTCTTTCCT TATGTAACAT GTTTCTGGAT
    GAAATGGCTA AGCAAGCTCG AAATCTTATC ACAGACATTT GCACAGAACA ATGTACGCTG
    AGTGATCAGT TGCTGCCAAA GCATTGTGCC AAAACCATCA GCCAAGCAGT GAACAAAAAA
    TCCAAAAAAC AGACAGGAAA GAAAGGAGAA CCTGAAAGGG AGAAACCAGG AGTAGAAAGC
    ATGAGAAAAA ACAGATTGGT TGTGACAAAC CTGGACAAAC TGCACACTGC ACTTTCGGAG
    CTCTGCTTCT CAATCAATTA TGTACCAAAC ATGGTGGTTT GGGAGCACAC ATTTACCCCA
    AGAGAATATT TAACGTCTCA CCTGGAAATC CGCTTTACCA AGTCCATTGT TGGAATGACG
    ATGTATAATC AAGCAACACA GGAGATTGCA AAGCCTTCAG AGCTGTTAAC CAGTGTAAGA
    GCCTATATGA CGGTCCTCCA GTCAATAGAA AACTATGTAC AGATTGACAT TACACGAGTA
    TTTAACAATG TTCTGCTTCA GCAAACTCAG CATTTAGATA GTCATGGTGA GCCAACCATC
    ACCAGTCTGT ACACAAATTG GTATTTGGAA ACATTGCTAA GGCAAGTCAG CAATGGTCAC
    ATAGCCTATT TTCCAGCTAT GAAGGCATTT GTGAATTTGC CAACAGAAAA TGAATTAACA
    TTTAATGCTG AGGAGTACTC TGACATATCA GAAATGAGAG CCCTTTCTGA ACTCCTGGGA
    CCATATGGCA TGAAGTTCTT AAGCGAAAGT CTGATGTGGC ACATTTCTTC GCAGGTTGCC
    GAGCTTAAGA AACTCGTGGT GGAGAATGTT GAAGTTCTAA CACAAATGAG GACCAGCTTC
    GATAAGCCAG ACCAGATGGC AGCACTCTTC AAAAGATTAT CATCTGTTGA CAGTGTTTTG
    AAGAGAATGA CGATTATTGG TGTCATCTTG TCTTTTCGGT CGTTGGCACA GGAAGCACTT
    AGAGATGTCT TATCCTACCA CATTCCTTTC CTTGTAAGTT CCATTGAGGA CTTCAAAGAT
    CACATTCCAA GAGAGACTGA CATGAAGGTG GCAATGAATG TATATGAACT GTCATCAGCT
    GCTGGATTGC CTTGCGAGAT TGATCCTGCC TTGGTTGTAG CACTGTCCTC CCAAAAATCA
    GAAAACATCA GTCCAGAAGA AGAATACAAA ATTGCTTGCC TTCTCATGGT CTTCGTGGCA
    GTCTCCTTGC CAACTTTGGC TAGTAACGTG ATGTCTCAGT ATAGCCCTGC CATTGAAGGG
    CACTGCAACA ACATTCATTG CTTGGCAAAA GCTATCAACC AGATTGCTGC AGCTTTATTT
    ACCATCCACA AGGGAAGCAT CGAAGACCGT CTAAAAGAAT TTTTGGCTCT TGCATCATCT
    AGTCTACTGA AGATCGGCCA GGAGACGGAC AAAACTACTA CAAGAAACAG AGAGTCTGTT
    TACCTACTAC TGGACATGAT TGTGCAGGAG TCCCCGTTCC TGACGATGGA CCTCCTGGAG
    TCGTGCTTCC CCTACGTCTT GCTGAGAAAC GCGTACCACG CTGTCTACAA ACAAAGCGTC
    ACCTCCTCTG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.