MCF2 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	MCF2
Entrez Gene ID	422253
Full Name	MCF.2 cell line derived transforming sequence
General protein information	Preferred Names MCF.2 cell line derived transforming sequence Names proto-oncogene DBL
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015278485.1	XP_015133971.1	proto-oncogene DBL isoform X2
XM_025150041.1	XP_025005809.1	proto-oncogene DBL isoform X2
XM_015278484.1	XP_015133970.1	proto-oncogene DBL isoform X1
XM_015278484.2	XP_015133970.2	proto-oncogene DBL isoform X1
XM_025150042.1	XP_025005810.1	proto-oncogene DBL isoform X2
XM_015278485.2	XP_015133971.1	proto-oncogene DBL isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MCF2	XP_003954149.1
Xenopus tropicalis (tropical clawed frog)	mcf2	XP_002932674.2
Mus musculus (house mouse)	Mcf2	NP_573460.2
Gallus gallus (chicken)	MCF2	XP_420239.4
Danio rerio (zebrafish)	mcf2a	XP_005173283.1
Homo sapiens (human)	MCF2	NP_001165347.1
Canis lupus familiaris (dog)	MCF2	XP_005641937.1
Rattus norvegicus (Norway rat)	Mcf2	XP_006257657.1
	MCF2	XP_002808597.1
Bos taurus (cattle)	MCF2	XP_005227614.1

Gene Ontology

The following MCF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MCF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa150369	XM_015278484.2 Latest version!	Gallus gallus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OGa150370	XM_025150042.1 Latest version!	Gallus gallus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OGa150370	XM_025150041.1 Latest version!	Gallus gallus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OGa34763	XM_015278484.1 Latest version!	Gallus gallus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OGa34764	XM_015278485.2 Latest version!	Gallus gallus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
OGa34764	XM_015278485.1	Gallus gallus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MCF2 ( XM_015278484.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015133970.2 Gallus gallus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa150369
Clone ID Related Accession (Same CDS sequence)	XM_015278484.2
Accession Version	XM_015278484.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	proto-oncogene DBL isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015278484.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGG CGCCGGCCCC GGCCGGGCCC CCGCCGGGGG GTACCTCGGC GGGGGAGATG 
GAGCGCTACT ACTGTCTGCT GCGGGCCGCC GCGCTGTTGG AGGCCGCCGC CGCCGAATCG 
ACAGCACCCA TCTGTGCGAA GGACATCAGC GATGAGTTGA TGAAGCAGTT TGCTTTTCTT 
TCTGGTGGCC GAGGGAAGGA CAAGGCTTGG ATCATCACCT TGCCTGACAA CTCTCGCTTC 
AACGATGTGC CTGAGGACGT TATAAGTAAA GTCTTAACGT ACCTAACATC GGTCCCGAGT 
CAACATGAGT CGGACACCAA ATTTATCCTA ATCCTGGATA GAAGATTGGA CACGTGGACA 
TCAGTCAAAA TGACTCTCCA AAGAATAGCA GCGTCATTCC CAGGGAACCT GCACTTGGTG 
ATGGTTCTTC GTCCCACAGG GTTTTTCCAA CGCACCTTCA CTGACATTGG ATTTCGATTT 
AGCCAGGAGG ATTTTCTGCT CAAGTTACCG GTTGTTATGC TGAGCTCAGT TAGCGACCTG 
CTGACATACA TTGATGAGCA GCAGCTAACC CCAGAGTTTG GAGGCACCTT GGAGTACTGC 
CACAGCGAGT GGGTCATCTT CAGGACTGCA ATAGAGAGTT TTGCACTAAC CGTGAAGGAA 
ATTGCACAGA TGTTACAATC TTTTGGCACA GAGCTGGCAG AGACTGAGCT ACCAGAGGAT 
ATGTATTCCA TTGAGCGCAT CCTGGCCCTG CGCACTGAGA AGTACTTCCA GCTCAAGGAA 
GATATTACAG CAGTCACAAA AGAAGGAAAG TTGCTTTTAA GCAATTTAGA AGATCCTGAT 
GTGGGTGAAT GCAGCCAGGA CCAGCACCGA GAGAGACCTG GAGACTGGGA GACTGTGAAT 
AGGCTACTCG GGCAGCTGCG GGAGATGGAG ACTGCTTTTG ATGGCTTCTG GGAAAAGCAT 
CAGTTGAAAA TGGAGCAGTA TTTACAACTG TGGAAGTTTG AGCAGAGTTT TCAAGAGGTC 
AAGAATGCCA TTGAATTTTT AATGGGTCAA CAAGCAAAAC TGCCTGACAC TGGAGACAGT 
GTCTCCCAAG TGAAACAGAG GCTGAAGGAC TTGAGTCATT TTGATGGTAT GGCTCAGGAT 
CTGATAGGGA AGGCTCAGGT GGTGATACTA CACGGACACC AGCTAGCAGC AAATCATCAC 
TATGCGCTCA ACTTAATCTG CCAGCAGTGC AACGAGCTGC GGCACCATTC TGATGTCTTG 
TCTGACGAGA TCAAAAGGAA GCAGATGCGG CTGCAGAAGA CCTTGGATCT TCATACTCGC 
CTTCAGCAGG CCCTGCAATG CTGTGATGAG GGTGCCTACC TGCTTGCCAA CCAGCAGATG 
GACAAGTGCC AGTCTAAAGA AGGTGCTCAG AAAGCTCTCC AGGACATAGA AAGATTTCTT 
GAAAGCTCTT CGCCCTACTT AAACTATGAT CCCCAATCCC TACAATATGA TTTTGAGTCA 
GTCTTAACAG CTGAATTGAA GTGCCAGATA CAGTCAGTGC AGGTGAAGCT TGAAAATGTT 
CGAAGCATGT TTGAGAACCG TCAGTCTTGC TTCAAGAAGC TGTTGGATAA GCATGTGCGT 
CCCGTCCAGT TAGTGGCCCC TCGGCCAGAA AACCCACCAC GATCCAAGTC ACCATTATTC 
TCTCCTAAAC ATGGTGTGGA TTTTAATTCC AGTTTGAAAT TTTCATTTGA TCTCTCTCTC 
CCTGGGAAGA AAACATCAAG GAAAACTCCA AGTTCTCGCA AGATCGAAGT AATGCACGAT 
TATCAGGAAA AACGGAATTC CTTGCAGTCT TTTATTTCAG ACAGTGATGA CAACTTGGAT 
ATACTGAAAG GCCACGTCAT AAATGAGCTT ATAGAAACTG AGAGAGTGTA CGTAGAGGAA 
CTGTTCACTG TTCTGACGGG CTACAGAGCT GAGATGGACA ACCCTGCCAT GGTCATCCTT 
ATGCCTCCCG TGCTGAGGAA CAGGAAGGAT GTCCTCTTTG GAAACATGCC TGAGATATAT 
GACTTTCACA ACAAAATATT CCTGCACAGT TTGGAAAGCT GCCTGGGAGC ACCCGAGAGA 
GTGGGATTTT GTTTCCTAGA CAGGCGAGAG GATTTCCAGA TGTATGAGAA ATACTGTCAG 
AACAAGCCCC GCTCAGAGTC CCTGTGGAGG CAGTGCTCGG AGAGCACCTT CTTCCAGGAG 
TGCCAAAGAA AATTAGAGCA CAAACTCGGG CTGGATTCCT ATTTGCTCAA ACCAGTGCAG 
CGCCTGACAA AATACCAGTT GCTTCTGAAG GAGTTGTTAA AGTACAGCAC AAGCTGTGAT 
GGAGTTCAGG AACTTCAAGA AGCTCTGGTT GCAATGCTGG ATTTGCTAAA ATCAGTCAAT 
GACTCTATGC ATCAGATTTC AATAACAGGA TATGACGGCG ACTTGAGTGA ACTAGGAAAA 
GTATTGATGC AAGGATCTTT CAGCGTTTGG ACAGGACACC GAAAAGGTCC AACAAAAATG 
AAGGATCTTG CCAGGTTCAA GCCAATGCAG AGGCATCTCT TCCTGTATGA GAAAGCCTTG 
GTCTTCTGCA AGAAGCGAGA GGATCATGGA GATGGATATG ATAAAACTTC ATCTTACAGT 
TTTAAGCATT TTCTGAAAAT GAATGCAGTT GGAATAACTG AGAACGTGAA GGGGGATCAT 
AGGAAATTTG AAATCTGGTA TAGTGGTCGA GAAGAGGTGT ATGTTGTGCA GGCCCAAACA 
GTAGATCTGA AGATGGCATG GTTGAATGAA ATCAGAAAAA TTTTATTCAA GCAGCAGGAG 
CTCATAAAAG TGGAGAAGCA GCAGCCTGGC TCATGCACTG ACCAGCTCCA CCTCTCCTCC 
CAGCTGAGCG ATGGAAAGCA GCAGAGAGCC TCCATAAGCT CAGAAGAGAA CGACTCAGAG 
CGCACCAGCC CGGTACCGTT GGACAGCGTG CTCCTGTCAC CCCAGAGCAA ACCCCACAGA 
AGCTGGCCAG GAATGTCACA GTCCCTGGAG ATCTGTGAGG GGCTGGAGGA GTGGTCGGGT 
AACCAGTACC TTTCCAACTG CTCGGACACC GAAGAGGAAG AGGGGAACCA GCTGTCTCCA 
GGAAAATACA AAGCCCTTGC AGACTGCAAG AGGAGAGGAT CAGAGGATCT GCTGGTGAAA 
AATGGGGATG AAATTCAGCT TTTGCATGAA GATGGCGAGG GACAGTGGTT GGTGAAAAAC 
CTAAACAGAA GAAAAGAAGG CTGGATCCCT GTCCACAGTC TGCAGATAGT AGTCGGTGAT 
TGTAGATTTC GGAATGCCAA AGTTGCAGAT GCTGCCCTGT GTAACACGCG AAAGTTTAGT 
TCCCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015133970.2
CDS	43..3411
Translation	MALAPAPAGPPPGGTSAGEMERYYCLLRAAALLEAAAAESTAPICAKDISDELMKQFAFLSGGRGKDKAWIITLPDNSRFNDVPEDVISKVLTYLTSVPSQHESDTKFILILDRRLDTWTSVKMTLQRIAASFPGNLHLVMVLRPTGFFQRTFTDIGFRFSQEDFLLKLPVVMLSSVSDLLTYIDEQQLTPEFGGTLEYCHSEWVIFRTAIESFALTVKEIAQMLQSFGTELAETELPEDMYSIERILALRTEKYFQLKEDITAVTKEGKLLLSNLEDPDVGECSQDQHRERPGDWETVNRLLGQLREMETAFDGFWEKHQLKMEQYLQLWKFEQSFQEVKNAIEFLMGQQAKLPDTGDSVSQVKQRLKDLSHFDGMAQDLIGKAQVVILHGHQLAANHHYALNLICQQCNELRHHSDVLSDEIKRKQMRLQKTLDLHTRLQQALQCCDEGAYLLANQQMDKCQSKEGAQKALQDIERFLESSSPYLNYDPQSLQYDFESVLTAELKCQIQSVQVKLENVRSMFENRQSCFKKLLDKHVRPVQLVAPRPENPPRSKSPLFSPKHGVDFNSSLKFSFDLSLPGKKTSRKTPSSRKIEVMHDYQEKRNSLQSFISDSDDNLDILKGHVINELIETERVYVEELFTVLTGYRAEMDNPAMVILMPPVLRNRKDVLFGNMPEIYDFHNKIFLHSLESCLGAPERVGFCFLDRREDFQMYEKYCQNKPRSESLWRQCSESTFFQECQRKLEHKLGLDSYLLKPVQRLTKYQLLLKELLKYSTSCDGVQELQEALVAMLDLLKSVNDSMHQISITGYDGDLSELGKVLMQGSFSVWTGHRKGPTKMKDLARFKPMQRHLFLYEKALVFCKKREDHGDGYDKTSSYSFKHFLKMNAVGITENVKGDHRKFEIWYSGREEVYVVQAQTVDLKMAWLNEIRKILFKQQELIKVEKQQPGSCTDQLHLSSQLSDGKQQRASISSEENDSERTSPVPLDSVLLSPQSKPHRSWPGMSQSLEICEGLEEWSGNQYLSNCSDTEEEEGNQLSPGKYKALADCKRRGSEDLLVKNGDEIQLLHEDGEGQWLVKNLNRRKEGWIPVHSLQIVVGDCRFRNAKVADAALCNTRKFSSP

Target ORF information:

RefSeq Version	XM_015278484.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015278484.2

ORF Insert Sequence:

ATGGCCCTGG CGCCGGCCCC GGCCGGGCCC CCGCCGGGGG GTACCTCGGC GGGGGAGATG 
GAGCGCTACT ACTGTCTGCT GCGGGCCGCC GCGCTGTTGG AGGCCGCCGC CGCCGAATCG 
ACAGCACCCA TCTGTGCGAA GGACATCAGC GATGAGTTGA TGAAGCAGTT TGCTTTTCTT 
TCTGGTGGCC GAGGGAAGGA CAAGGCTTGG ATCATCACCT TGCCTGACAA CTCTCGCTTC 
AACGATGTGC CTGAGGACGT TATAAGTAAA GTCTTAACGT ACCTAACATC GGTCCCGAGT 
CAACATGAGT CGGACACCAA ATTTATCCTA ATCCTGGATA GAAGATTGGA CACGTGGACA 
TCAGTCAAAA TGACTCTCCA AAGAATAGCA GCGTCATTCC CAGGGAACCT GCACTTGGTG 
ATGGTTCTTC GTCCCACAGG GTTTTTCCAA CGCACCTTCA CTGACATTGG ATTTCGATTT 
AGCCAGGAGG ATTTTCTGCT CAAGTTACCG GTTGTTATGC TGAGCTCAGT TAGCGACCTG 
CTGACATACA TTGATGAGCA GCAGCTAACC CCAGAGTTTG GAGGCACCTT GGAGTACTGC 
CACAGCGAGT GGGTCATCTT CAGGACTGCA ATAGAGAGTT TTGCACTAAC CGTGAAGGAA 
ATTGCACAGA TGTTACAATC TTTTGGCACA GAGCTGGCAG AGACTGAGCT ACCAGAGGAT 
ATGTATTCCA TTGAGCGCAT CCTGGCCCTG CGCACTGAGA AGTACTTCCA GCTCAAGGAA 
GATATTACAG CAGTCACAAA AGAAGGAAAG TTGCTTTTAA GCAATTTAGA AGATCCTGAT 
GTGGGTGAAT GCAGCCAGGA CCAGCACCGA GAGAGACCTG GAGACTGGGA GACTGTGAAT 
AGGCTACTCG GGCAGCTGCG GGAGATGGAG ACTGCTTTTG ATGGCTTCTG GGAAAAGCAT 
CAGTTGAAAA TGGAGCAGTA TTTACAACTG TGGAAGTTTG AGCAGAGTTT TCAAGAGGTC 
AAGAATGCCA TTGAATTTTT AATGGGTCAA CAAGCAAAAC TGCCTGACAC TGGAGACAGT 
GTCTCCCAAG TGAAACAGAG GCTGAAGGAC TTGAGTCATT TTGATGGTAT GGCTCAGGAT 
CTGATAGGGA AGGCTCAGGT GGTGATACTA CACGGACACC AGCTAGCAGC AAATCATCAC 
TATGCGCTCA ACTTAATCTG CCAGCAGTGC AACGAGCTGC GGCACCATTC TGATGTCTTG 
TCTGACGAGA TCAAAAGGAA GCAGATGCGG CTGCAGAAGA CCTTGGATCT TCATACTCGC 
CTTCAGCAGG CCCTGCAATG CTGTGATGAG GGTGCCTACC TGCTTGCCAA CCAGCAGATG 
GACAAGTGCC AGTCTAAAGA AGGTGCTCAG AAAGCTCTCC AGGACATAGA AAGATTTCTT 
GAAAGCTCTT CGCCCTACTT AAACTATGAT CCCCAATCCC TACAATATGA TTTTGAGTCA 
GTCTTAACAG CTGAATTGAA GTGCCAGATA CAGTCAGTGC AGGTGAAGCT TGAAAATGTT 
CGAAGCATGT TTGAGAACCG TCAGTCTTGC TTCAAGAAGC TGTTGGATAA GCATGTGCGT 
CCCGTCCAGT TAGTGGCCCC TCGGCCAGAA AACCCACCAC GATCCAAGTC ACCATTATTC 
TCTCCTAAAC ATGGTGTGGA TTTTAATTCC AGTTTGAAAT TTTCATTTGA TCTCTCTCTC 
CCTGGGAAGA AAACATCAAG GAAAACTCCA AGTTCTCGCA AGATCGAAGT AATGCACGAT 
TATCAGGAAA AACGGAATTC CTTGCAGTCT TTTATTTCAG ACAGTGATGA CAACTTGGAT 
ATACTGAAAG GCCACGTCAT AAATGAGCTT ATAGAAACTG AGAGAGTGTA CGTAGAGGAA 
CTGTTCACTG TTCTGACGGG CTACAGAGCT GAGATGGACA ACCCTGCCAT GGTCATCCTT 
ATGCCTCCCG TGCTGAGGAA CAGGAAGGAT GTCCTCTTTG GAAACATGCC TGAGATATAT 
GACTTTCACA ACAAAATATT CCTGCACAGT TTGGAAAGCT GCCTGGGAGC ACCCGAGAGA 
GTGGGATTTT GTTTCCTAGA CAGGCGAGAG GATTTCCAGA TGTATGAGAA ATACTGTCAG 
AACAAGCCCC GCTCAGAGTC CCTGTGGAGG CAGTGCTCGG AGAGCACCTT CTTCCAGGAG 
TGCCAAAGAA AATTAGAGCA CAAACTCGGG CTGGATTCCT ATTTGCTCAA ACCAGTGCAG 
CGCCTGACAA AATACCAGTT GCTTCTGAAG GAGTTGTTAA AGTACAGCAC AAGCTGTGAT 
GGAGTTCAGG AACTTCAAGA AGCTCTGGTT GCAATGCTGG ATTTGCTAAA ATCAGTCAAT 
GACTCTATGC ATCAGATTTC AATAACAGGA TATGACGGCG ACTTGAGTGA ACTAGGAAAA 
GTATTGATGC AAGGATCTTT CAGCGTTTGG ACAGGACACC GAAAAGGTCC AACAAAAATG 
AAGGATCTTG CCAGGTTCAA GCCAATGCAG AGGCATCTCT TCCTGTATGA GAAAGCCTTG 
GTCTTCTGCA AGAAGCGAGA GGATCATGGA GATGGATATG ATAAAACTTC ATCTTACAGT 
TTTAAGCATT TTCTGAAAAT GAATGCAGTT GGAATAACTG AGAACGTGAA GGGGGATCAT 
AGGAAATTTG AAATCTGGTA TAGTGGTCGA GAAGAGGTGT ATGTTGTGCA GGCCCAAACA 
GTAGATCTGA AGATGGCATG GTTGAATGAA ATCAGAAAAA TTTTATTCAA GCAGCAGGAG 
CTCATAAAAG TGGAGAAGCA GCAGCCTGGC TCATGCACTG ACCAGCTCCA CCTCTCCTCC 
CAGCTGAGCG ATGGAAAGCA GCAGAGAGCC TCCATAAGCT CAGAAGAGAA CGACTCAGAG 
CGCACCAGCC CGGTACCGTT GGACAGCGTG CTCCTGTCAC CCCAGAGCAA ACCCCACAGA 
AGCTGGCCAG GAATGTCACA GTCCCTGGAG ATCTGTGAGG GGCTGGAGGA GTGGTCGGGT 
AACCAGTACC TTTCCAACTG CTCGGACACC GAAGAGGAAG AGGGGAACCA GCTGTCTCCA 
GGAAAATACA AAGCCCTTGC AGACTGCAAG AGGAGAGGAT CAGAGGATCT GCTGGTGAAA 
AATGGGGATG AAATTCAGCT TTTGCATGAA GATGGCGAGG GACAGTGGTT GGTGAAAAAC 
CTAAACAGAA GAAAAGAAGG CTGGATCCCT GTCCACAGTC TGCAGATAGT AGTCGGTGAT 
TGTAGATTTC GGAATGCCAA AGTTGCAGAT GCTGCCCTGT GTAACACGCG AAAGTTTAGT 
TCCCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2 ( XM_025150041.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025005809.1 Gallus gallus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa150370
Clone ID Related Accession (Same CDS sequence)	XM_025150041.1 , XM_025150042.1
Accession Version	XM_025150041.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	proto-oncogene DBL isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGG CGCCGGCCCC GGCCGGGCCC CCGCCGGGGG GTACCTCGGC GGGGGAGATG 
GAGCGCTACT ACTGTCTGCT GCGGGCCGCC GCGCTGTTGG AGGCCGCCGC CGCCGAATCG 
ACAGCACCCA TCTGTGCGAA GGACATCAGC GATGAGTTGA TGAAGCAGTT TGCTTTTCTT 
TCTGGTGGCC GAGGGAAGGA CAAGGCTTGG ATCATCACCT TGCCTGACAA CTCTCGCTTC 
AACGATGTGC CTGAGGACGT TATAAGTAAA GTCTTAACGT ACCTAACATC GGTCCCGAGT 
CAACATGAGT CGGACACCAA ATTTATCCTA ATCCTGGATA GAAGATTGGA CACGTGGACA 
TCAGTCAAAA TGACTCTCCA AAGAATAGCA GCGTCATTCC CAGGGAACCT GCACTTGGTG 
ATGGTTCTTC GTCCCACAGG GTTTTTCCAA CGCACCTTCA CTGACATTGG ATTTCGATTT 
AGCCAGGAGG ATTTTCTGCT CAAGTTACCG GTTGTTATGC TGAGCTCAGT TAGCGACCTG 
CTGACATACA TTGATGAGCA GCAGCTAACC CCAGAGTTTG GAGGCACCTT GGAGTACTGC 
CACAGCGAGT GGGTCATCTT CAGGACTGCA ATAGAGAGTT TTGCACTAAC CGTGAAGGAA 
ATTGCACAGA TGTTACAATC TTTTGGCACA GAGCTGGCAG AGACTGAGCT ACCAGAGGAT 
ATGTATTCCA TTGAGCGCAT CCTGGCCCTG CGCACTGAGA AGTACTTCCA GCTCAAGGAA 
GATATTACAG CAGTCACAAA AGAAGGAAAG TTGCTTTTAA GCAATTTAGA AGATCCTGAT 
GTGGGTGAAT GCAGCCAGGA CCAGCACCGA GAGAGACCTG GAGACTGGGA GACTGTGAAT 
AGGCTACTCG GGCAGCTGCG GGAGATGGAG ACTGCTTTTG ATGGCTTCTG GGAAAAGCAT 
CAGTTGAAAA TGGAGCAGTA TTTACAACTG TGGAAGTTTG AGCAGAGTTT TCAAGAGGTC 
AAGAATGCCA TTGAATTTTT AATGGGTCAA CAAGCAAAAC TGCCTGACAC TGGAGACAGT 
GTCTCCCAAG TGAAACAGAG GCTGAAGGAC TTGAGTCATT TTGATGGTAT GGCTCAGGAT 
CTGATAGGGA AGGCTCAGGT GGTGATACTA CACGGACACC AGCTAGCAGC AAATCATCAC 
TATGCGCTCA ACTTAATCTG CCAGCAGTGC AACGAGCTGC GGCACCATTC TGATGTCTTG 
TCTGACGAGA TCAAAAGGAA GCAGATGCGG CTGCAGAAGA CCTTGGATCT TCATACTCGC 
CTTCAGCAGG CCCTGCAATG CTGTGATGAG GGTGCCTACC TGCTTGCCAA CCAGCAGATG 
GACAAGTGCC AGTCTAAAGA AGGTGCTCAG AAAGCTCTCC AGGACATAGA AAGATTTCTT 
GAAAGCTCTT CGCCCTACTT AAACTATGAT CCCCAATCCC TACAATATGA TTTTGAGTCA 
GTCTTAACAG CTGAATTGAA GTGCCAGATA CAGTCAGTGC AGGTGAAGCT TGAAAATGTT 
CGAAGCATGT TTGAGAACCG TCAGTCTTGC TTCAAGAAGC TGTTGGATAA GCATGTGCGT 
CCCGTCCAGT TAGTGGCCCC TCGGCCAGAA AACCCACCAC GATCCAAGTC ACCATTATTC 
TCTCCTAAAC ATGGTGTGGA TTTTAATTCC AGTTTGAAAT TTTCATTTGA TCTCTCTCTC 
CCTGGGAAGA AAACATCAAG GAAAACTCCA AGTTCTCGCA AGATCGAAGT AATGCACGAT 
TATCAGGAAA AACGGAATTC CTTGCAGTCT TTTATTTCAG ACAGTGATGA CAACTTGGAT 
ATACTGAAAG GCCACGTCAT AAATGAGCTT ATAGAAACTG AGAGAGTGTA CGTAGAGGAA 
CTGTTCACTG TTCTGACGGG CTACAGAGCT GAGATGGACA ACCCTGCCAT GGTCATCCTT 
ATGCCTCCCG TGCTGAGGAA CAGGAAGGAT GTCCTCTTTG GAAACATGCC TGAGATATAT 
GACTTTCACA ACAAAATATT CCTGCACAGT TTGGAAAGCT GCCTGGGAGC ACCCGAGAGA 
GTGGGATTTT GTTTCCTAGA CAGGCGAGAG GATTTCCAGA TGTATGAGAA ATACTGTCAG 
AACAAGCCCC GCTCAGAGTC CCTGTGGAGG CAGTGCTCGG AGAGCACCTT CTTCCAGGAG 
TGCCAAAGAA AATTAGAGCA CAAACTCGGG CTGGATTCCT ATTTGCTCAA ACCAGTGCAG 
CGCCTGACAA AATACCAGTT GCTTCTGAAG GAGTTGTTAA AGTACAGCAC AAGCTGTGAT 
GGAGTTCAGG AACTTCAAGA AGCTCTGGTT GCAATGCTGG ATTTGCTAAA ATCAGTCAAT 
GACTCTATGC ATCAGATTTC AATAACAGGA TATGACGGCG ACTTGAGTGA ACTAGGAAAA 
GTATTGATGC AAGGATCTTT CAGCGTTTGG ACAGGACACC GAAAAGGTCC AACAAAAATG 
AAGGATCTTG CCAGGTTCAA GCCAATGCAG AGGCATCTCT TCCTGTATGA GAAAGCCTTG 
GTCTTCTGCA AGAAGCGAGA GGATCATGGA GATGGATATG ATAAAACTTC ATCTTACAGT 
TTTAAGCATT TTCTGAAAAT GAATGCAGTT GGAATAACTG AGAACGTGAA GGGGGATCAT 
AGGAAATTTG AAATCTGGTA TAGTGGTCGA GAAGAGGTGT ATGTTGTGCA GGCCCAAACA 
GTAGATCTGA AGATGGCATG GTTGAATGAA ATCAGAAAAA TTTTATTCAA GCAGCAGGAG 
CTCATAAAAG TGGAGAAGCA GCAGCCTGGC TCATGCACTG ACCAGCTCCA CCTCTCCTCC 
CAGCTGAGCG ATGGAAAGCA GCAGAGAGCC TCCATAAGCT CAGAAGAGAA CGACTCAGAG 
CGCACCAGCC CGGTACCGTT GGACAGCGTG CTCCTGTCAC CCCAGAGCAA ACCCCACAGA 
AGCTGGCCAG GAATGTCACA GTCCCTGGAG ATCTGTGAGG GGCTGGAGGA GTGGTCGGGT 
AACCAGTACC TTTCCAACTG CTCGGACACC GAAGAGGAAG AGGGGAACCA GCTGTCTCCA 
GGAAAATACA AAGCCCTTGC AGACTGCAAG AGGAGAGGAT CAGAGGATCT GCTGGTGAAA 
AATGGGGATG AAATTCAGCT TTTGCATGAA GATGGCGAGG GACAGTGGTT GGTGAAAAAC 
CTAAACAGAA GAAAAGAAGG CTGGATCCCT GTCCACAGTC TGCAGATAGT AGTCGGTGAT 
TGTAGATTTC GGAATGCCAA AGTTGCAGAT GTTAACGCAT GGATCTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025005809.1
CDS	43..3393
Translation	MALAPAPAGPPPGGTSAGEMERYYCLLRAAALLEAAAAESTAPICAKDISDELMKQFAFLSGGRGKDKAWIITLPDNSRFNDVPEDVISKVLTYLTSVPSQHESDTKFILILDRRLDTWTSVKMTLQRIAASFPGNLHLVMVLRPTGFFQRTFTDIGFRFSQEDFLLKLPVVMLSSVSDLLTYIDEQQLTPEFGGTLEYCHSEWVIFRTAIESFALTVKEIAQMLQSFGTELAETELPEDMYSIERILALRTEKYFQLKEDITAVTKEGKLLLSNLEDPDVGECSQDQHRERPGDWETVNRLLGQLREMETAFDGFWEKHQLKMEQYLQLWKFEQSFQEVKNAIEFLMGQQAKLPDTGDSVSQVKQRLKDLSHFDGMAQDLIGKAQVVILHGHQLAANHHYALNLICQQCNELRHHSDVLSDEIKRKQMRLQKTLDLHTRLQQALQCCDEGAYLLANQQMDKCQSKEGAQKALQDIERFLESSSPYLNYDPQSLQYDFESVLTAELKCQIQSVQVKLENVRSMFENRQSCFKKLLDKHVRPVQLVAPRPENPPRSKSPLFSPKHGVDFNSSLKFSFDLSLPGKKTSRKTPSSRKIEVMHDYQEKRNSLQSFISDSDDNLDILKGHVINELIETERVYVEELFTVLTGYRAEMDNPAMVILMPPVLRNRKDVLFGNMPEIYDFHNKIFLHSLESCLGAPERVGFCFLDRREDFQMYEKYCQNKPRSESLWRQCSESTFFQECQRKLEHKLGLDSYLLKPVQRLTKYQLLLKELLKYSTSCDGVQELQEALVAMLDLLKSVNDSMHQISITGYDGDLSELGKVLMQGSFSVWTGHRKGPTKMKDLARFKPMQRHLFLYEKALVFCKKREDHGDGYDKTSSYSFKHFLKMNAVGITENVKGDHRKFEIWYSGREEVYVVQAQTVDLKMAWLNEIRKILFKQQELIKVEKQQPGSCTDQLHLSSQLSDGKQQRASISSEENDSERTSPVPLDSVLLSPQSKPHRSWPGMSQSLEICEGLEEWSGNQYLSNCSDTEEEEGNQLSPGKYKALADCKRRGSEDLLVKNGDEIQLLHEDGEGQWLVKNLNRRKEGWIPVHSLQIVVGDCRFRNAKVADVNAWIS

Target ORF information:

RefSeq Version	XM_025150041.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025150041.1

ORF Insert Sequence:

ATGGCCCTGG CGCCGGCCCC GGCCGGGCCC CCGCCGGGGG GTACCTCGGC GGGGGAGATG 
GAGCGCTACT ACTGTCTGCT GCGGGCCGCC GCGCTGTTGG AGGCCGCCGC CGCCGAATCG 
ACAGCACCCA TCTGTGCGAA GGACATCAGC GATGAGTTGA TGAAGCAGTT TGCTTTTCTT 
TCTGGTGGCC GAGGGAAGGA CAAGGCTTGG ATCATCACCT TGCCTGACAA CTCTCGCTTC 
AACGATGTGC CTGAGGACGT TATAAGTAAA GTCTTAACGT ACCTAACATC GGTCCCGAGT 
CAACATGAGT CGGACACCAA ATTTATCCTA ATCCTGGATA GAAGATTGGA CACGTGGACA 
TCAGTCAAAA TGACTCTCCA AAGAATAGCA GCGTCATTCC CAGGGAACCT GCACTTGGTG 
ATGGTTCTTC GTCCCACAGG GTTTTTCCAA CGCACCTTCA CTGACATTGG ATTTCGATTT 
AGCCAGGAGG ATTTTCTGCT CAAGTTACCG GTTGTTATGC TGAGCTCAGT TAGCGACCTG 
CTGACATACA TTGATGAGCA GCAGCTAACC CCAGAGTTTG GAGGCACCTT GGAGTACTGC 
CACAGCGAGT GGGTCATCTT CAGGACTGCA ATAGAGAGTT TTGCACTAAC CGTGAAGGAA 
ATTGCACAGA TGTTACAATC TTTTGGCACA GAGCTGGCAG AGACTGAGCT ACCAGAGGAT 
ATGTATTCCA TTGAGCGCAT CCTGGCCCTG CGCACTGAGA AGTACTTCCA GCTCAAGGAA 
GATATTACAG CAGTCACAAA AGAAGGAAAG TTGCTTTTAA GCAATTTAGA AGATCCTGAT 
GTGGGTGAAT GCAGCCAGGA CCAGCACCGA GAGAGACCTG GAGACTGGGA GACTGTGAAT 
AGGCTACTCG GGCAGCTGCG GGAGATGGAG ACTGCTTTTG ATGGCTTCTG GGAAAAGCAT 
CAGTTGAAAA TGGAGCAGTA TTTACAACTG TGGAAGTTTG AGCAGAGTTT TCAAGAGGTC 
AAGAATGCCA TTGAATTTTT AATGGGTCAA CAAGCAAAAC TGCCTGACAC TGGAGACAGT 
GTCTCCCAAG TGAAACAGAG GCTGAAGGAC TTGAGTCATT TTGATGGTAT GGCTCAGGAT 
CTGATAGGGA AGGCTCAGGT GGTGATACTA CACGGACACC AGCTAGCAGC AAATCATCAC 
TATGCGCTCA ACTTAATCTG CCAGCAGTGC AACGAGCTGC GGCACCATTC TGATGTCTTG 
TCTGACGAGA TCAAAAGGAA GCAGATGCGG CTGCAGAAGA CCTTGGATCT TCATACTCGC 
CTTCAGCAGG CCCTGCAATG CTGTGATGAG GGTGCCTACC TGCTTGCCAA CCAGCAGATG 
GACAAGTGCC AGTCTAAAGA AGGTGCTCAG AAAGCTCTCC AGGACATAGA AAGATTTCTT 
GAAAGCTCTT CGCCCTACTT AAACTATGAT CCCCAATCCC TACAATATGA TTTTGAGTCA 
GTCTTAACAG CTGAATTGAA GTGCCAGATA CAGTCAGTGC AGGTGAAGCT TGAAAATGTT 
CGAAGCATGT TTGAGAACCG TCAGTCTTGC TTCAAGAAGC TGTTGGATAA GCATGTGCGT 
CCCGTCCAGT TAGTGGCCCC TCGGCCAGAA AACCCACCAC GATCCAAGTC ACCATTATTC 
TCTCCTAAAC ATGGTGTGGA TTTTAATTCC AGTTTGAAAT TTTCATTTGA TCTCTCTCTC 
CCTGGGAAGA AAACATCAAG GAAAACTCCA AGTTCTCGCA AGATCGAAGT AATGCACGAT 
TATCAGGAAA AACGGAATTC CTTGCAGTCT TTTATTTCAG ACAGTGATGA CAACTTGGAT 
ATACTGAAAG GCCACGTCAT AAATGAGCTT ATAGAAACTG AGAGAGTGTA CGTAGAGGAA 
CTGTTCACTG TTCTGACGGG CTACAGAGCT GAGATGGACA ACCCTGCCAT GGTCATCCTT 
ATGCCTCCCG TGCTGAGGAA CAGGAAGGAT GTCCTCTTTG GAAACATGCC TGAGATATAT 
GACTTTCACA ACAAAATATT CCTGCACAGT TTGGAAAGCT GCCTGGGAGC ACCCGAGAGA 
GTGGGATTTT GTTTCCTAGA CAGGCGAGAG GATTTCCAGA TGTATGAGAA ATACTGTCAG 
AACAAGCCCC GCTCAGAGTC CCTGTGGAGG CAGTGCTCGG AGAGCACCTT CTTCCAGGAG 
TGCCAAAGAA AATTAGAGCA CAAACTCGGG CTGGATTCCT ATTTGCTCAA ACCAGTGCAG 
CGCCTGACAA AATACCAGTT GCTTCTGAAG GAGTTGTTAA AGTACAGCAC AAGCTGTGAT 
GGAGTTCAGG AACTTCAAGA AGCTCTGGTT GCAATGCTGG ATTTGCTAAA ATCAGTCAAT 
GACTCTATGC ATCAGATTTC AATAACAGGA TATGACGGCG ACTTGAGTGA ACTAGGAAAA 
GTATTGATGC AAGGATCTTT CAGCGTTTGG ACAGGACACC GAAAAGGTCC AACAAAAATG 
AAGGATCTTG CCAGGTTCAA GCCAATGCAG AGGCATCTCT TCCTGTATGA GAAAGCCTTG 
GTCTTCTGCA AGAAGCGAGA GGATCATGGA GATGGATATG ATAAAACTTC ATCTTACAGT 
TTTAAGCATT TTCTGAAAAT GAATGCAGTT GGAATAACTG AGAACGTGAA GGGGGATCAT 
AGGAAATTTG AAATCTGGTA TAGTGGTCGA GAAGAGGTGT ATGTTGTGCA GGCCCAAACA 
GTAGATCTGA AGATGGCATG GTTGAATGAA ATCAGAAAAA TTTTATTCAA GCAGCAGGAG 
CTCATAAAAG TGGAGAAGCA GCAGCCTGGC TCATGCACTG ACCAGCTCCA CCTCTCCTCC 
CAGCTGAGCG ATGGAAAGCA GCAGAGAGCC TCCATAAGCT CAGAAGAGAA CGACTCAGAG 
CGCACCAGCC CGGTACCGTT GGACAGCGTG CTCCTGTCAC CCCAGAGCAA ACCCCACAGA 
AGCTGGCCAG GAATGTCACA GTCCCTGGAG ATCTGTGAGG GGCTGGAGGA GTGGTCGGGT 
AACCAGTACC TTTCCAACTG CTCGGACACC GAAGAGGAAG AGGGGAACCA GCTGTCTCCA 
GGAAAATACA AAGCCCTTGC AGACTGCAAG AGGAGAGGAT CAGAGGATCT GCTGGTGAAA 
AATGGGGATG AAATTCAGCT TTTGCATGAA GATGGCGAGG GACAGTGGTT GGTGAAAAAC 
CTAAACAGAA GAAAAGAAGG CTGGATCCCT GTCCACAGTC TGCAGATAGT AGTCGGTGAT 
TGTAGATTTC GGAATGCCAA AGTTGCAGAT GTTAACGCAT GGATCTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2 ( XM_025150042.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025005810.1 Gallus gallus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa150370
Clone ID Related Accession (Same CDS sequence)	XM_025150041.1 , XM_025150042.1
Accession Version	XM_025150042.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	proto-oncogene DBL isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGG CGCCGGCCCC GGCCGGGCCC CCGCCGGGGG GTACCTCGGC GGGGGAGATG 
GAGCGCTACT ACTGTCTGCT GCGGGCCGCC GCGCTGTTGG AGGCCGCCGC CGCCGAATCG 
ACAGCACCCA TCTGTGCGAA GGACATCAGC GATGAGTTGA TGAAGCAGTT TGCTTTTCTT 
TCTGGTGGCC GAGGGAAGGA CAAGGCTTGG ATCATCACCT TGCCTGACAA CTCTCGCTTC 
AACGATGTGC CTGAGGACGT TATAAGTAAA GTCTTAACGT ACCTAACATC GGTCCCGAGT 
CAACATGAGT CGGACACCAA ATTTATCCTA ATCCTGGATA GAAGATTGGA CACGTGGACA 
TCAGTCAAAA TGACTCTCCA AAGAATAGCA GCGTCATTCC CAGGGAACCT GCACTTGGTG 
ATGGTTCTTC GTCCCACAGG GTTTTTCCAA CGCACCTTCA CTGACATTGG ATTTCGATTT 
AGCCAGGAGG ATTTTCTGCT CAAGTTACCG GTTGTTATGC TGAGCTCAGT TAGCGACCTG 
CTGACATACA TTGATGAGCA GCAGCTAACC CCAGAGTTTG GAGGCACCTT GGAGTACTGC 
CACAGCGAGT GGGTCATCTT CAGGACTGCA ATAGAGAGTT TTGCACTAAC CGTGAAGGAA 
ATTGCACAGA TGTTACAATC TTTTGGCACA GAGCTGGCAG AGACTGAGCT ACCAGAGGAT 
ATGTATTCCA TTGAGCGCAT CCTGGCCCTG CGCACTGAGA AGTACTTCCA GCTCAAGGAA 
GATATTACAG CAGTCACAAA AGAAGGAAAG TTGCTTTTAA GCAATTTAGA AGATCCTGAT 
GTGGGTGAAT GCAGCCAGGA CCAGCACCGA GAGAGACCTG GAGACTGGGA GACTGTGAAT 
AGGCTACTCG GGCAGCTGCG GGAGATGGAG ACTGCTTTTG ATGGCTTCTG GGAAAAGCAT 
CAGTTGAAAA TGGAGCAGTA TTTACAACTG TGGAAGTTTG AGCAGAGTTT TCAAGAGGTC 
AAGAATGCCA TTGAATTTTT AATGGGTCAA CAAGCAAAAC TGCCTGACAC TGGAGACAGT 
GTCTCCCAAG TGAAACAGAG GCTGAAGGAC TTGAGTCATT TTGATGGTAT GGCTCAGGAT 
CTGATAGGGA AGGCTCAGGT GGTGATACTA CACGGACACC AGCTAGCAGC AAATCATCAC 
TATGCGCTCA ACTTAATCTG CCAGCAGTGC AACGAGCTGC GGCACCATTC TGATGTCTTG 
TCTGACGAGA TCAAAAGGAA GCAGATGCGG CTGCAGAAGA CCTTGGATCT TCATACTCGC 
CTTCAGCAGG CCCTGCAATG CTGTGATGAG GGTGCCTACC TGCTTGCCAA CCAGCAGATG 
GACAAGTGCC AGTCTAAAGA AGGTGCTCAG AAAGCTCTCC AGGACATAGA AAGATTTCTT 
GAAAGCTCTT CGCCCTACTT AAACTATGAT CCCCAATCCC TACAATATGA TTTTGAGTCA 
GTCTTAACAG CTGAATTGAA GTGCCAGATA CAGTCAGTGC AGGTGAAGCT TGAAAATGTT 
CGAAGCATGT TTGAGAACCG TCAGTCTTGC TTCAAGAAGC TGTTGGATAA GCATGTGCGT 
CCCGTCCAGT TAGTGGCCCC TCGGCCAGAA AACCCACCAC GATCCAAGTC ACCATTATTC 
TCTCCTAAAC ATGGTGTGGA TTTTAATTCC AGTTTGAAAT TTTCATTTGA TCTCTCTCTC 
CCTGGGAAGA AAACATCAAG GAAAACTCCA AGTTCTCGCA AGATCGAAGT AATGCACGAT 
TATCAGGAAA AACGGAATTC CTTGCAGTCT TTTATTTCAG ACAGTGATGA CAACTTGGAT 
ATACTGAAAG GCCACGTCAT AAATGAGCTT ATAGAAACTG AGAGAGTGTA CGTAGAGGAA 
CTGTTCACTG TTCTGACGGG CTACAGAGCT GAGATGGACA ACCCTGCCAT GGTCATCCTT 
ATGCCTCCCG TGCTGAGGAA CAGGAAGGAT GTCCTCTTTG GAAACATGCC TGAGATATAT 
GACTTTCACA ACAAAATATT CCTGCACAGT TTGGAAAGCT GCCTGGGAGC ACCCGAGAGA 
GTGGGATTTT GTTTCCTAGA CAGGCGAGAG GATTTCCAGA TGTATGAGAA ATACTGTCAG 
AACAAGCCCC GCTCAGAGTC CCTGTGGAGG CAGTGCTCGG AGAGCACCTT CTTCCAGGAG 
TGCCAAAGAA AATTAGAGCA CAAACTCGGG CTGGATTCCT ATTTGCTCAA ACCAGTGCAG 
CGCCTGACAA AATACCAGTT GCTTCTGAAG GAGTTGTTAA AGTACAGCAC AAGCTGTGAT 
GGAGTTCAGG AACTTCAAGA AGCTCTGGTT GCAATGCTGG ATTTGCTAAA ATCAGTCAAT 
GACTCTATGC ATCAGATTTC AATAACAGGA TATGACGGCG ACTTGAGTGA ACTAGGAAAA 
GTATTGATGC AAGGATCTTT CAGCGTTTGG ACAGGACACC GAAAAGGTCC AACAAAAATG 
AAGGATCTTG CCAGGTTCAA GCCAATGCAG AGGCATCTCT TCCTGTATGA GAAAGCCTTG 
GTCTTCTGCA AGAAGCGAGA GGATCATGGA GATGGATATG ATAAAACTTC ATCTTACAGT 
TTTAAGCATT TTCTGAAAAT GAATGCAGTT GGAATAACTG AGAACGTGAA GGGGGATCAT 
AGGAAATTTG AAATCTGGTA TAGTGGTCGA GAAGAGGTGT ATGTTGTGCA GGCCCAAACA 
GTAGATCTGA AGATGGCATG GTTGAATGAA ATCAGAAAAA TTTTATTCAA GCAGCAGGAG 
CTCATAAAAG TGGAGAAGCA GCAGCCTGGC TCATGCACTG ACCAGCTCCA CCTCTCCTCC 
CAGCTGAGCG ATGGAAAGCA GCAGAGAGCC TCCATAAGCT CAGAAGAGAA CGACTCAGAG 
CGCACCAGCC CGGTACCGTT GGACAGCGTG CTCCTGTCAC CCCAGAGCAA ACCCCACAGA 
AGCTGGCCAG GAATGTCACA GTCCCTGGAG ATCTGTGAGG GGCTGGAGGA GTGGTCGGGT 
AACCAGTACC TTTCCAACTG CTCGGACACC GAAGAGGAAG AGGGGAACCA GCTGTCTCCA 
GGAAAATACA AAGCCCTTGC AGACTGCAAG AGGAGAGGAT CAGAGGATCT GCTGGTGAAA 
AATGGGGATG AAATTCAGCT TTTGCATGAA GATGGCGAGG GACAGTGGTT GGTGAAAAAC 
CTAAACAGAA GAAAAGAAGG CTGGATCCCT GTCCACAGTC TGCAGATAGT AGTCGGTGAT 
TGTAGATTTC GGAATGCCAA AGTTGCAGAT GTTAACGCAT GGATCTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025005810.1
CDS	43..3393
Translation	MALAPAPAGPPPGGTSAGEMERYYCLLRAAALLEAAAAESTAPICAKDISDELMKQFAFLSGGRGKDKAWIITLPDNSRFNDVPEDVISKVLTYLTSVPSQHESDTKFILILDRRLDTWTSVKMTLQRIAASFPGNLHLVMVLRPTGFFQRTFTDIGFRFSQEDFLLKLPVVMLSSVSDLLTYIDEQQLTPEFGGTLEYCHSEWVIFRTAIESFALTVKEIAQMLQSFGTELAETELPEDMYSIERILALRTEKYFQLKEDITAVTKEGKLLLSNLEDPDVGECSQDQHRERPGDWETVNRLLGQLREMETAFDGFWEKHQLKMEQYLQLWKFEQSFQEVKNAIEFLMGQQAKLPDTGDSVSQVKQRLKDLSHFDGMAQDLIGKAQVVILHGHQLAANHHYALNLICQQCNELRHHSDVLSDEIKRKQMRLQKTLDLHTRLQQALQCCDEGAYLLANQQMDKCQSKEGAQKALQDIERFLESSSPYLNYDPQSLQYDFESVLTAELKCQIQSVQVKLENVRSMFENRQSCFKKLLDKHVRPVQLVAPRPENPPRSKSPLFSPKHGVDFNSSLKFSFDLSLPGKKTSRKTPSSRKIEVMHDYQEKRNSLQSFISDSDDNLDILKGHVINELIETERVYVEELFTVLTGYRAEMDNPAMVILMPPVLRNRKDVLFGNMPEIYDFHNKIFLHSLESCLGAPERVGFCFLDRREDFQMYEKYCQNKPRSESLWRQCSESTFFQECQRKLEHKLGLDSYLLKPVQRLTKYQLLLKELLKYSTSCDGVQELQEALVAMLDLLKSVNDSMHQISITGYDGDLSELGKVLMQGSFSVWTGHRKGPTKMKDLARFKPMQRHLFLYEKALVFCKKREDHGDGYDKTSSYSFKHFLKMNAVGITENVKGDHRKFEIWYSGREEVYVVQAQTVDLKMAWLNEIRKILFKQQELIKVEKQQPGSCTDQLHLSSQLSDGKQQRASISSEENDSERTSPVPLDSVLLSPQSKPHRSWPGMSQSLEICEGLEEWSGNQYLSNCSDTEEEEGNQLSPGKYKALADCKRRGSEDLLVKNGDEIQLLHEDGEGQWLVKNLNRRKEGWIPVHSLQIVVGDCRFRNAKVADVNAWIS

Target ORF information:

RefSeq Version	XM_025150042.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025150042.1

ORF Insert Sequence:

ATGGCCCTGG CGCCGGCCCC GGCCGGGCCC CCGCCGGGGG GTACCTCGGC GGGGGAGATG 
GAGCGCTACT ACTGTCTGCT GCGGGCCGCC GCGCTGTTGG AGGCCGCCGC CGCCGAATCG 
ACAGCACCCA TCTGTGCGAA GGACATCAGC GATGAGTTGA TGAAGCAGTT TGCTTTTCTT 
TCTGGTGGCC GAGGGAAGGA CAAGGCTTGG ATCATCACCT TGCCTGACAA CTCTCGCTTC 
AACGATGTGC CTGAGGACGT TATAAGTAAA GTCTTAACGT ACCTAACATC GGTCCCGAGT 
CAACATGAGT CGGACACCAA ATTTATCCTA ATCCTGGATA GAAGATTGGA CACGTGGACA 
TCAGTCAAAA TGACTCTCCA AAGAATAGCA GCGTCATTCC CAGGGAACCT GCACTTGGTG 
ATGGTTCTTC GTCCCACAGG GTTTTTCCAA CGCACCTTCA CTGACATTGG ATTTCGATTT 
AGCCAGGAGG ATTTTCTGCT CAAGTTACCG GTTGTTATGC TGAGCTCAGT TAGCGACCTG 
CTGACATACA TTGATGAGCA GCAGCTAACC CCAGAGTTTG GAGGCACCTT GGAGTACTGC 
CACAGCGAGT GGGTCATCTT CAGGACTGCA ATAGAGAGTT TTGCACTAAC CGTGAAGGAA 
ATTGCACAGA TGTTACAATC TTTTGGCACA GAGCTGGCAG AGACTGAGCT ACCAGAGGAT 
ATGTATTCCA TTGAGCGCAT CCTGGCCCTG CGCACTGAGA AGTACTTCCA GCTCAAGGAA 
GATATTACAG CAGTCACAAA AGAAGGAAAG TTGCTTTTAA GCAATTTAGA AGATCCTGAT 
GTGGGTGAAT GCAGCCAGGA CCAGCACCGA GAGAGACCTG GAGACTGGGA GACTGTGAAT 
AGGCTACTCG GGCAGCTGCG GGAGATGGAG ACTGCTTTTG ATGGCTTCTG GGAAAAGCAT 
CAGTTGAAAA TGGAGCAGTA TTTACAACTG TGGAAGTTTG AGCAGAGTTT TCAAGAGGTC 
AAGAATGCCA TTGAATTTTT AATGGGTCAA CAAGCAAAAC TGCCTGACAC TGGAGACAGT 
GTCTCCCAAG TGAAACAGAG GCTGAAGGAC TTGAGTCATT TTGATGGTAT GGCTCAGGAT 
CTGATAGGGA AGGCTCAGGT GGTGATACTA CACGGACACC AGCTAGCAGC AAATCATCAC 
TATGCGCTCA ACTTAATCTG CCAGCAGTGC AACGAGCTGC GGCACCATTC TGATGTCTTG 
TCTGACGAGA TCAAAAGGAA GCAGATGCGG CTGCAGAAGA CCTTGGATCT TCATACTCGC 
CTTCAGCAGG CCCTGCAATG CTGTGATGAG GGTGCCTACC TGCTTGCCAA CCAGCAGATG 
GACAAGTGCC AGTCTAAAGA AGGTGCTCAG AAAGCTCTCC AGGACATAGA AAGATTTCTT 
GAAAGCTCTT CGCCCTACTT AAACTATGAT CCCCAATCCC TACAATATGA TTTTGAGTCA 
GTCTTAACAG CTGAATTGAA GTGCCAGATA CAGTCAGTGC AGGTGAAGCT TGAAAATGTT 
CGAAGCATGT TTGAGAACCG TCAGTCTTGC TTCAAGAAGC TGTTGGATAA GCATGTGCGT 
CCCGTCCAGT TAGTGGCCCC TCGGCCAGAA AACCCACCAC GATCCAAGTC ACCATTATTC 
TCTCCTAAAC ATGGTGTGGA TTTTAATTCC AGTTTGAAAT TTTCATTTGA TCTCTCTCTC 
CCTGGGAAGA AAACATCAAG GAAAACTCCA AGTTCTCGCA AGATCGAAGT AATGCACGAT 
TATCAGGAAA AACGGAATTC CTTGCAGTCT TTTATTTCAG ACAGTGATGA CAACTTGGAT 
ATACTGAAAG GCCACGTCAT AAATGAGCTT ATAGAAACTG AGAGAGTGTA CGTAGAGGAA 
CTGTTCACTG TTCTGACGGG CTACAGAGCT GAGATGGACA ACCCTGCCAT GGTCATCCTT 
ATGCCTCCCG TGCTGAGGAA CAGGAAGGAT GTCCTCTTTG GAAACATGCC TGAGATATAT 
GACTTTCACA ACAAAATATT CCTGCACAGT TTGGAAAGCT GCCTGGGAGC ACCCGAGAGA 
GTGGGATTTT GTTTCCTAGA CAGGCGAGAG GATTTCCAGA TGTATGAGAA ATACTGTCAG 
AACAAGCCCC GCTCAGAGTC CCTGTGGAGG CAGTGCTCGG AGAGCACCTT CTTCCAGGAG 
TGCCAAAGAA AATTAGAGCA CAAACTCGGG CTGGATTCCT ATTTGCTCAA ACCAGTGCAG 
CGCCTGACAA AATACCAGTT GCTTCTGAAG GAGTTGTTAA AGTACAGCAC AAGCTGTGAT 
GGAGTTCAGG AACTTCAAGA AGCTCTGGTT GCAATGCTGG ATTTGCTAAA ATCAGTCAAT 
GACTCTATGC ATCAGATTTC AATAACAGGA TATGACGGCG ACTTGAGTGA ACTAGGAAAA 
GTATTGATGC AAGGATCTTT CAGCGTTTGG ACAGGACACC GAAAAGGTCC AACAAAAATG 
AAGGATCTTG CCAGGTTCAA GCCAATGCAG AGGCATCTCT TCCTGTATGA GAAAGCCTTG 
GTCTTCTGCA AGAAGCGAGA GGATCATGGA GATGGATATG ATAAAACTTC ATCTTACAGT 
TTTAAGCATT TTCTGAAAAT GAATGCAGTT GGAATAACTG AGAACGTGAA GGGGGATCAT 
AGGAAATTTG AAATCTGGTA TAGTGGTCGA GAAGAGGTGT ATGTTGTGCA GGCCCAAACA 
GTAGATCTGA AGATGGCATG GTTGAATGAA ATCAGAAAAA TTTTATTCAA GCAGCAGGAG 
CTCATAAAAG TGGAGAAGCA GCAGCCTGGC TCATGCACTG ACCAGCTCCA CCTCTCCTCC 
CAGCTGAGCG ATGGAAAGCA GCAGAGAGCC TCCATAAGCT CAGAAGAGAA CGACTCAGAG 
CGCACCAGCC CGGTACCGTT GGACAGCGTG CTCCTGTCAC CCCAGAGCAA ACCCCACAGA 
AGCTGGCCAG GAATGTCACA GTCCCTGGAG ATCTGTGAGG GGCTGGAGGA GTGGTCGGGT 
AACCAGTACC TTTCCAACTG CTCGGACACC GAAGAGGAAG AGGGGAACCA GCTGTCTCCA 
GGAAAATACA AAGCCCTTGC AGACTGCAAG AGGAGAGGAT CAGAGGATCT GCTGGTGAAA 
AATGGGGATG AAATTCAGCT TTTGCATGAA GATGGCGAGG GACAGTGGTT GGTGAAAAAC 
CTAAACAGAA GAAAAGAAGG CTGGATCCCT GTCCACAGTC TGCAGATAGT AGTCGGTGAT 
TGTAGATTTC GGAATGCCAA AGTTGCAGAT GTTAACGCAT GGATCTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2 ( XM_015278484.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015133970.1 Gallus gallus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa34763
Clone ID Related Accession (Same CDS sequence)	XM_015278484.1
Accession Version	XM_015278484.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	proto-oncogene DBL isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763735.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGCT ACTACTGTCT GCTGCGGGCC GCCGCGCTGT TGGAGGCCGC CGCCGCCGAA 
TCGACAGCAC CCATCTGTGC GAAGGACATC AGCGATGAGT TGATGAAGCA GTTTGCTTTT 
CTTTCTGGTG GCCGAGGGAA GGACAAGGCT TGGATCATCA CCTTGCCTGA CAACTCTCGC 
TTCAACGATG TGCCTGAGGA CGTTATAAGT AAAGTCTTAA CGTACCTAAC ATCGGTCCCG 
AGTCAACATG AGTCGGACAC CAAATTTATC CTAATCCTGG ATAGAAGATT GGACACGTGG 
ACATCAGTCA AAATGACTCT CCAAAGAATA GCAGCGTCAT TCCCAGGGAA CCTGCACTTG 
GTGATGGTTC TTCGTCCCAC AGGGTTTTTC CAACGCACCT TCACTGACAT TGGATTTCGA 
TTTAGCCAGG AGGATTTTCT GCTCAAGTTA CCGGTTGTTA TGCTGAGCTC AGTTAGCGAC 
CTGCTGACAT ACATTGATGA GCAGCAGCTA ACCCCAGAGT TTGGAGGCAC CTTGGAGTAC 
TGCCACAGCG AGTGGGTCAT CTTCAGGACT GCAATAGAGA GTTTTGCACT AACCGTGAAG 
GAAATTGCAC AGATGTTACA ATCTTTTGGC ACAGAGCTGG CAGAGACTGA GCTACCAGAG 
GATATGTATT CCATTGAGCG CATCCTGGCC CTGCGCACTG AGAAGTACTT CCAGCTCAAG 
GAAGATATTA CAGCAGTCAC AAAAGAAGGA AAGTTGCTTT TAAGCAATTT AGAAGATCCT 
GATGTGGGTG AATGCAGCCA GGACCAGCAC CGAGAGAGAC CTGGAGACTG GGAGACTGTG 
AATAGGCTAC TCGGGCAGCT GCGGGAGATG GAGACTGCTT TTGATGGCTT CTGGGAAAAG 
CATCAGTTGA AAATGGAGCA GTATTTACAA CTGTGGAAGT TTGAGCAGAG TTTTCAAGAG 
GTCAAGAATG CCATTGAATT TTTAATGGGT CAACAAGCAA AACTGCCTGA CACTGGAGAC 
AGTGTCTCCC AAGTGAAACA GAGGCTGAAG GACTTGAGTC ATTTTGATGG TATGGCTCAG 
GATCTGATAG GGAAGGCTCA GGTGGTGATA CTACACGGAC ACCAGCTAGC AGCAAATCAT 
CACTATGCGC TCAACTTAAT CTGCCAGCAG TGCAACGAGC TGCGGCACCA TTCTGATGTC 
TTGTCTGACG AGATCAAAAG GAAGCAGATG CGGCTGCAGA AGACCTTGGA TCTTCATACT 
CGCCTTCAGC AGGCCCTGCA ATGCTGTGAT GAGGGTGCCT ACCTGCTTGC CAACCAGCAG 
ATGGACAAGT GCCAGTCTAA AGAAGGTGCT CAGAAAGCTC TCCAGGACAT AGAAAGATTT 
CTTGAAAGCT CTTCGCCCTA CTTAAACTAT GATCCCCAAT CCCTACAATA TGATTTTGAG 
TCAGTCTTAA CAGCTGAATT GAAGTGCCAG ATACAGTCAG TGCAGGTGAA GCTTGAAAAT 
GTTCGAAGCA TGTTTGAGAA CCGTCAGTCT TGCTTCAAGA AGCTGTTGGA TAAGCATGTG 
CGTCCCGTCC AGTTAGTGGC CCCTCGGCCA GAAAACCCAC CACGATCCAA GTCACCATTA 
TTCTCTCCTA AACATGGTGT GGATTTTAAT TCCAGTTTGA AATTTTCATT TGATCTCTCT 
CTCCCTGGGA AGAAAACATC AAGGAAAACT CCAAGTTCTC GCAAGATCGA AGTAATGCAC 
GATTATCAGG AAAAACGGAA TTCCTTGCAG TCTTTTATTT CAGACAGTGA TGACAACTTG 
GATATACTGA AAGGCCACGT CATAAATGAG CTTATAGAAA CTGAGAGAGT GTACGTAGAG 
GAACTGTTCA CTGTTCTGAC GGGCTACAGA GCTGAGATGG ACAACCCTGC CATGGTCATC 
CTTATGCCTC CCGTGCTGAG GAACAGGAAG GATGTCCTCT TTGGAAACAT GCCTGAGATA 
TATGACTTTC ACAACAAAAT ATTCCTGCAC AGTTTGGAAA GCTGCCTGGG AGCACCCGAG 
AGAGTGGGAT TTTGTTTCCT AGACAGGCGA GAGGATTTCC AGATGTATGA GAAATACTGT 
CAGAACAAGC CCCGCTCAGA GTCCCTGTGG AGGCAGTGCT CGGAGAGCAC CTTCTTCCAG 
GAGTGCCAAA GAAAATTAGA GCACAAACTC GGGCTGGATT CCTATTTGCT CAAACCAGTG 
CAGCGCCTGA CAAAATACCA GTTGCTTCTG AAGGAGTTGT TAAAGTACAG CACAAGCTGT 
GATGGAGTTC AGGAACTTCA AGAAGCTCTG GTTGCAATGC TGGATTTGCT AAAATCAGTC 
AATGACTCTA TGCATCAGAT TTCAATAACA GGATATGACG GCGACTTGAG TGAACTAGGA 
AAAGTATTGA TGCAAGGATC TTTCAGCGTT TGGACAGGAC ACCGAAAAGG TCCAACAAAA 
ATGAAGGATC TTGCCAGGTT CAAGCCAATG CAGAGGCATC TCTTCCTGTA TGAGAAAGCC 
TTGGTCTTCT GCAAGAAGCG AGAGGATCAT GGAGATGGAT ATGATAAAAC TTCATCTTAC 
AGTTTTAAGC ATTTTCTGAA AATGAATGCA GTTGGAATAA CTGAGAACGT GAAGGGGGAT 
CATAGGAAAT TTGAAATCTG GTATAGTGGT CGAGAAGAGG TGTATGTTGT GCAGGCCCAA 
ACAGTAGATC TGAAGATGGC ATGGTTGAAT GAAATCAGAA AAATTTTATT CAAGCAGCAG 
GAGCTCATAA AAGTGGAGAA GCAGCAGCCT GGCTCATGCA CTGACCAGCT CCACCTCTCC 
TCCCAGCTGA GCGATGGAAA GCAGCAGAGA GCCTCCATAA GCTCAGAAGA GAACGACTCA 
GAGCGCACCA GCCCGGTACC GTTGGACAGC GTGCTCCTGT CACCCCAGAG CAAACCCCAC 
AGAAGCTGGC CAGGAATGTC ACAGTCCCTG GAGATCTGTG AGGGGCTGGA GGAGTGGTCG 
GGTAACCAGT ACCTTTCCAA CTGCTCGGAC ACCGAAGAGG AAGAGGGGAA CCAGCTGTCT 
CCAGGAAAAT ACAAAGCCCT TGCAGACTGC AAGAGGAGAG GATCAGAGGA TCTGCTGGTG 
AAAAATGGGG ATGAAATTCA GCTTTTGCAT GAAGATGGCG AGGGACAGTG GTTGGTGAAA 
AACCTAAACA GAAGAAAAGA AGGCTGGATC CCTGTCCACA GTCTGCAGAT AGTAGTCGGT 
GATTGTAGAT TTCGGAATGC CAAAGTTGCA GATGCTGCCC TGTGTAACAC GCGAAAGTTT 
AGTTCCCCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015133970.1
CDS	85..3396
Translation	MERYYCLLRAAALLEAAAAESTAPICAKDISDELMKQFAFLSGGRGKDKAWIITLPDNSRFNDVPEDVISKVLTYLTSVPSQHESDTKFILILDRRLDTWTSVKMTLQRIAASFPGNLHLVMVLRPTGFFQRTFTDIGFRFSQEDFLLKLPVVMLSSVSDLLTYIDEQQLTPEFGGTLEYCHSEWVIFRTAIESFALTVKEIAQMLQSFGTELAETELPEDMYSIERILALRTEKYFQLKEDITAVTKEGKLLLSNLEDPDVGECSQDQHRERPGDWETVNRLLGQLREMETAFDGFWEKHQLKMEQYLQLWKFEQSFQEVKNAIEFLMGQQAKLPDTGDSVSQVKQRLKDLSHFDGMAQDLIGKAQVVILHGHQLAANHHYALNLICQQCNELRHHSDVLSDEIKRKQMRLQKTLDLHTRLQQALQCCDEGAYLLANQQMDKCQSKEGAQKALQDIERFLESSSPYLNYDPQSLQYDFESVLTAELKCQIQSVQVKLENVRSMFENRQSCFKKLLDKHVRPVQLVAPRPENPPRSKSPLFSPKHGVDFNSSLKFSFDLSLPGKKTSRKTPSSRKIEVMHDYQEKRNSLQSFISDSDDNLDILKGHVINELIETERVYVEELFTVLTGYRAEMDNPAMVILMPPVLRNRKDVLFGNMPEIYDFHNKIFLHSLESCLGAPERVGFCFLDRREDFQMYEKYCQNKPRSESLWRQCSESTFFQECQRKLEHKLGLDSYLLKPVQRLTKYQLLLKELLKYSTSCDGVQELQEALVAMLDLLKSVNDSMHQISITGYDGDLSELGKVLMQGSFSVWTGHRKGPTKMKDLARFKPMQRHLFLYEKALVFCKKREDHGDGYDKTSSYSFKHFLKMNAVGITENVKGDHRKFEIWYSGREEVYVVQAQTVDLKMAWLNEIRKILFKQQELIKVEKQQPGSCTDQLHLSSQLSDGKQQRASISSEENDSERTSPVPLDSVLLSPQSKPHRSWPGMSQSLEICEGLEEWSGNQYLSNCSDTEEEEGNQLSPGKYKALADCKRRGSEDLLVKNGDEIQLLHEDGEGQWLVKNLNRRKEGWIPVHSLQIVVGDCRFRNAKVADAALCNTRKFSSP

Target ORF information:

RefSeq Version	XM_015278484.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015278484.1

ORF Insert Sequence:

ATGGAGCGCT ACTACTGTCT GCTGCGGGCC GCCGCGCTGT TGGAGGCCGC CGCCGCCGAA 
TCGACAGCAC CCATCTGTGC GAAGGACATC AGCGATGAGT TGATGAAGCA GTTTGCTTTT 
CTTTCTGGTG GCCGAGGGAA GGACAAGGCT TGGATCATCA CCTTGCCTGA CAACTCTCGC 
TTCAACGATG TGCCTGAGGA CGTTATAAGT AAAGTCTTAA CGTACCTAAC ATCGGTCCCG 
AGTCAACATG AGTCGGACAC CAAATTTATC CTAATCCTGG ATAGAAGATT GGACACGTGG 
ACATCAGTCA AAATGACTCT CCAAAGAATA GCAGCGTCAT TCCCAGGGAA CCTGCACTTG 
GTGATGGTTC TTCGTCCCAC AGGGTTTTTC CAACGCACCT TCACTGACAT TGGATTTCGA 
TTTAGCCAGG AGGATTTTCT GCTCAAGTTA CCGGTTGTTA TGCTGAGCTC AGTTAGCGAC 
CTGCTGACAT ACATTGATGA GCAGCAGCTA ACCCCAGAGT TTGGAGGCAC CTTGGAGTAC 
TGCCACAGCG AGTGGGTCAT CTTCAGGACT GCAATAGAGA GTTTTGCACT AACCGTGAAG 
GAAATTGCAC AGATGTTACA ATCTTTTGGC ACAGAGCTGG CAGAGACTGA GCTACCAGAG 
GATATGTATT CCATTGAGCG CATCCTGGCC CTGCGCACTG AGAAGTACTT CCAGCTCAAG 
GAAGATATTA CAGCAGTCAC AAAAGAAGGA AAGTTGCTTT TAAGCAATTT AGAAGATCCT 
GATGTGGGTG AATGCAGCCA GGACCAGCAC CGAGAGAGAC CTGGAGACTG GGAGACTGTG 
AATAGGCTAC TCGGGCAGCT GCGGGAGATG GAGACTGCTT TTGATGGCTT CTGGGAAAAG 
CATCAGTTGA AAATGGAGCA GTATTTACAA CTGTGGAAGT TTGAGCAGAG TTTTCAAGAG 
GTCAAGAATG CCATTGAATT TTTAATGGGT CAACAAGCAA AACTGCCTGA CACTGGAGAC 
AGTGTCTCCC AAGTGAAACA GAGGCTGAAG GACTTGAGTC ATTTTGATGG TATGGCTCAG 
GATCTGATAG GGAAGGCTCA GGTGGTGATA CTACACGGAC ACCAGCTAGC AGCAAATCAT 
CACTATGCGC TCAACTTAAT CTGCCAGCAG TGCAACGAGC TGCGGCACCA TTCTGATGTC 
TTGTCTGACG AGATCAAAAG GAAGCAGATG CGGCTGCAGA AGACCTTGGA TCTTCATACT 
CGCCTTCAGC AGGCCCTGCA ATGCTGTGAT GAGGGTGCCT ACCTGCTTGC CAACCAGCAG 
ATGGACAAGT GCCAGTCTAA AGAAGGTGCT CAGAAAGCTC TCCAGGACAT AGAAAGATTT 
CTTGAAAGCT CTTCGCCCTA CTTAAACTAT GATCCCCAAT CCCTACAATA TGATTTTGAG 
TCAGTCTTAA CAGCTGAATT GAAGTGCCAG ATACAGTCAG TGCAGGTGAA GCTTGAAAAT 
GTTCGAAGCA TGTTTGAGAA CCGTCAGTCT TGCTTCAAGA AGCTGTTGGA TAAGCATGTG 
CGTCCCGTCC AGTTAGTGGC CCCTCGGCCA GAAAACCCAC CACGATCCAA GTCACCATTA 
TTCTCTCCTA AACATGGTGT GGATTTTAAT TCCAGTTTGA AATTTTCATT TGATCTCTCT 
CTCCCTGGGA AGAAAACATC AAGGAAAACT CCAAGTTCTC GCAAGATCGA AGTAATGCAC 
GATTATCAGG AAAAACGGAA TTCCTTGCAG TCTTTTATTT CAGACAGTGA TGACAACTTG 
GATATACTGA AAGGCCACGT CATAAATGAG CTTATAGAAA CTGAGAGAGT GTACGTAGAG 
GAACTGTTCA CTGTTCTGAC GGGCTACAGA GCTGAGATGG ACAACCCTGC CATGGTCATC 
CTTATGCCTC CCGTGCTGAG GAACAGGAAG GATGTCCTCT TTGGAAACAT GCCTGAGATA 
TATGACTTTC ACAACAAAAT ATTCCTGCAC AGTTTGGAAA GCTGCCTGGG AGCACCCGAG 
AGAGTGGGAT TTTGTTTCCT AGACAGGCGA GAGGATTTCC AGATGTATGA GAAATACTGT 
CAGAACAAGC CCCGCTCAGA GTCCCTGTGG AGGCAGTGCT CGGAGAGCAC CTTCTTCCAG 
GAGTGCCAAA GAAAATTAGA GCACAAACTC GGGCTGGATT CCTATTTGCT CAAACCAGTG 
CAGCGCCTGA CAAAATACCA GTTGCTTCTG AAGGAGTTGT TAAAGTACAG CACAAGCTGT 
GATGGAGTTC AGGAACTTCA AGAAGCTCTG GTTGCAATGC TGGATTTGCT AAAATCAGTC 
AATGACTCTA TGCATCAGAT TTCAATAACA GGATATGACG GCGACTTGAG TGAACTAGGA 
AAAGTATTGA TGCAAGGATC TTTCAGCGTT TGGACAGGAC ACCGAAAAGG TCCAACAAAA 
ATGAAGGATC TTGCCAGGTT CAAGCCAATG CAGAGGCATC TCTTCCTGTA TGAGAAAGCC 
TTGGTCTTCT GCAAGAAGCG AGAGGATCAT GGAGATGGAT ATGATAAAAC TTCATCTTAC 
AGTTTTAAGC ATTTTCTGAA AATGAATGCA GTTGGAATAA CTGAGAACGT GAAGGGGGAT 
CATAGGAAAT TTGAAATCTG GTATAGTGGT CGAGAAGAGG TGTATGTTGT GCAGGCCCAA 
ACAGTAGATC TGAAGATGGC ATGGTTGAAT GAAATCAGAA AAATTTTATT CAAGCAGCAG 
GAGCTCATAA AAGTGGAGAA GCAGCAGCCT GGCTCATGCA CTGACCAGCT CCACCTCTCC 
TCCCAGCTGA GCGATGGAAA GCAGCAGAGA GCCTCCATAA GCTCAGAAGA GAACGACTCA 
GAGCGCACCA GCCCGGTACC GTTGGACAGC GTGCTCCTGT CACCCCAGAG CAAACCCCAC 
AGAAGCTGGC CAGGAATGTC ACAGTCCCTG GAGATCTGTG AGGGGCTGGA GGAGTGGTCG 
GGTAACCAGT ACCTTTCCAA CTGCTCGGAC ACCGAAGAGG AAGAGGGGAA CCAGCTGTCT 
CCAGGAAAAT ACAAAGCCCT TGCAGACTGC AAGAGGAGAG GATCAGAGGA TCTGCTGGTG 
AAAAATGGGG ATGAAATTCA GCTTTTGCAT GAAGATGGCG AGGGACAGTG GTTGGTGAAA 
AACCTAAACA GAAGAAAAGA AGGCTGGATC CCTGTCCACA GTCTGCAGAT AGTAGTCGGT 
GATTGTAGAT TTCGGAATGC CAAAGTTGCA GATGCTGCCC TGTGTAACAC GCGAAAGTTT 
AGTTCCCCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2 ( XM_015278485.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015133971.1 Gallus gallus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X2, mRNA.

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CloneID	OGa34764
Clone ID Related Accession (Same CDS sequence)	XM_015278485.1 , XM_015278485.2
Accession Version	XM_015278485.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	proto-oncogene DBL isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763735.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CGAGCCCCGC GCGGGGCGTC AGGAGGCCGC GGAGGGATGC GGCGTCATTC 
CCAGGGAACC TGCACTTGGT GATGGTTCTT CGTCCCACAG GGTTTTTCCA ACGCACCTTC 
ACTGACATTG GATTTCGATT TAGCCAGGAG GATTTTCTGC TCAAGTTACC GGTTGTTATG 
CTGAGCTCAG TTAGCGACCT GCTGACATAC ATTGATGAGC AGCAGCTAAC CCCAGAGTTT 
GGAGGCACCT TGGAGTACTG CCACAGCGAG TGGGTCATCT TCAGGACTGC AATAGAGAGT 
TTTGCACTAA CCGTGAAGGA AATTGCACAG ATGTTACAAT CTTTTGGCAC AGAGCTGGCA 
GAGACTGAGC TACCAGAGGA TATGTATTCC ATTGAGCGCA TCCTGGCCCT GCGCACTGAG 
AAGTACTTCC AGCTCAAGGA AGATATTACA GCAGTCACAA AAGAAGGAAA GTTGCTTTTA 
AGCAATTTAG AAGATCCTGA TGTGGGTGAA TGCAGCCAGG ACCAGCACCG AGAGAGACCT 
GGAGACTGGG AGACTGTGAA TAGGCTACTC GGGCAGCTGC GGGAGATGGA GACTGCTTTT 
GATGGCTTCT GGGAAAAGCA TCAGTTGAAA ATGGAGCAGT ATTTACAACT GTGGAAGTTT 
GAGCAGAGTT TTCAAGAGGT CAAGAATGCC ATTGAATTTT TAATGGGTCA ACAAGCAAAA 
CTGCCTGACA CTGGAGACAG TGTCTCCCAA GTGAAACAGA GGCTGAAGGA CTTGAGTCAT 
TTTGATGGTA TGGCTCAGGA TCTGATAGGG AAGGCTCAGG TGGTGATACT ACACGGACAC 
CAGCTAGCAG CAAATCATCA CTATGCGCTC AACTTAATCT GCCAGCAGTG CAACGAGCTG 
CGGCACCATT CTGATGTCTT GTCTGACGAG ATCAAAAGGA AGCAGATGCG GCTGCAGAAG 
ACCTTGGATC TTCATACTCG CCTTCAGCAG GCCCTGCAAT GCTGTGATGA GGGTGCCTAC 
CTGCTTGCCA ACCAGCAGAT GGACAAGTGC CAGTCTAAAG AAGGTGCTCA GAAAGCTCTC 
CAGGACATAG AAAGATTTCT TGAAAGCTCT TCGCCCTACT TAAACTATGA TCCCCAATCC 
CTACAATATG ATTTTGAGTC AGTCTTAACA GCTGAATTGA AGTGCCAGAT ACAGTCAGTG 
CAGGTGAAGC TTGAAAATGT TCGAAGCATG TTTGAGAACC GTCAGTCTTG CTTCAAGAAG 
CTGTTGGATA AGCATGTGCG TCCCGTCCAG TTAGTGGCCC CTCGGCCAGA AAACCCACCA 
CGATCCAAGT CACCATTATT CTCTCCTAAA CATGGTGTGG ATTTTAATTC CAGTTTGAAA 
TTTTCATTTG ATCTCTCTCT CCCTGGGAAG AAAACATCAA GGAAAACTCC AAGTTCTCGC 
AAGATCGAAG TAATGCACGA TTATCAGGAA AAACGGAATT CCTTGCAGTC TTTTATTTCA 
GACAGTGATG ACAACTTGGA TATACTGAAA GGCCACGTCA TAAATGAGCT TATAGAAACT 
GAGAGAGTGT ACGTAGAGGA ACTGTTCACT GTTCTGACGG GCTACAGAGC TGAGATGGAC 
AACCCTGCCA TGGTCATCCT TATGCCTCCC GTGCTGAGGA ACAGGAAGGA TGTCCTCTTT 
GGAAACATGC CTGAGATATA TGACTTTCAC AACAAAATAT TCCTGCACAG TTTGGAAAGC 
TGCCTGGGAG CACCCGAGAG AGTGGGATTT TGTTTCCTAG ACAGGCGAGA GGATTTCCAG 
ATGTATGAGA AATACTGTCA GAACAAGCCC CGCTCAGAGT CCCTGTGGAG GCAGTGCTCG 
GAGAGCACCT TCTTCCAGGA GTGCCAAAGA AAATTAGAGC ACAAACTCGG GCTGGATTCC 
TATTTGCTCA AACCAGTGCA GCGCCTGACA AAATACCAGT TGCTTCTGAA GGAGTTGTTA 
AAGTACAGCA CAAGCTGTGA TGGAGTTCAG GAACTTCAAG AAGCTCTGGT TGCAATGCTG 
GATTTGCTAA AATCAGTCAA TGACTCTATG CATCAGATTT CAATAACAGG ATATGACGGC 
GACTTGAGTG AACTAGGAAA AGTATTGATG CAAGGATCTT TCAGCGTTTG GACAGGACAC 
CGAAAAGGTC CAACAAAAAT GAAGGATCTT GCCAGGTTCA AGCCAATGCA GAGGCATCTC 
TTCCTGTATG AGAAAGCCTT GGTCTTCTGC AAGAAGCGAG AGGATCATGG AGATGGATAT 
GATAAAACTT CATCTTACAG TTTTAAGCAT TTTCTGAAAA TGAATGCAGT TGGAATAACT 
GAGAACGTGA AGGGGGATCA TAGGAAATTT GAAATCTGGT ATAGTGGTCG AGAAGAGGTG 
TATGTTGTGC AGGCCCAAAC AGTAGATCTG AAGATGGCAT GGTTGAATGA AATCAGAAAA 
ATTTTATTCA AGCAGCAGGA GCTCATAAAA GTGGAGAAGC AGCAGCCTGG CTCATGCACT 
GACCAGCTCC ACCTCTCCTC CCAGCTGAGC GATGGAAAGC AGCAGAGAGC CTCCATAAGC 
TCAGAAGAGA ACGACTCAGA GCGCACCAGC CCGGTACCGT TGGACAGCGT GCTCCTGTCA 
CCCCAGAGCA AACCCCACAG AAGCTGGCCA GGAATGTCAC AGTCCCTGGA GATCTGTGAG 
GGGCTGGAGG AGTGGTCGGG TAACCAGTAC CTTTCCAACT GCTCGGACAC CGAAGAGGAA 
GAGGGGAACC AGCTGTCTCC AGGAAAATAC AAAGCCCTTG CAGACTGCAA GAGGAGAGGA 
TCAGAGGATC TGCTGGTGAA AAATGGGGAT GAAATTCAGC TTTTGCATGA AGATGGCGAG 
GGACAGTGGT TGGTGAAAAA CCTAAACAGA AGAAAAGAAG GCTGGATCCC TGTCCACAGT 
CTGCAGATAG TAGTCGGTGA TTGTAGATTT CGGAATGCCA AAGTTGCAGA TGCTGCCCTG 
TGTAACACGC GAAAGTTTAG TTCCCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015133971.1
CDS	53..3082
Translation	MAEASPARGVRRPRRDAASFPGNLHLVMVLRPTGFFQRTFTDIGFRFSQEDFLLKLPVVMLSSVSDLLTYIDEQQLTPEFGGTLEYCHSEWVIFRTAIESFALTVKEIAQMLQSFGTELAETELPEDMYSIERILALRTEKYFQLKEDITAVTKEGKLLLSNLEDPDVGECSQDQHRERPGDWETVNRLLGQLREMETAFDGFWEKHQLKMEQYLQLWKFEQSFQEVKNAIEFLMGQQAKLPDTGDSVSQVKQRLKDLSHFDGMAQDLIGKAQVVILHGHQLAANHHYALNLICQQCNELRHHSDVLSDEIKRKQMRLQKTLDLHTRLQQALQCCDEGAYLLANQQMDKCQSKEGAQKALQDIERFLESSSPYLNYDPQSLQYDFESVLTAELKCQIQSVQVKLENVRSMFENRQSCFKKLLDKHVRPVQLVAPRPENPPRSKSPLFSPKHGVDFNSSLKFSFDLSLPGKKTSRKTPSSRKIEVMHDYQEKRNSLQSFISDSDDNLDILKGHVINELIETERVYVEELFTVLTGYRAEMDNPAMVILMPPVLRNRKDVLFGNMPEIYDFHNKIFLHSLESCLGAPERVGFCFLDRREDFQMYEKYCQNKPRSESLWRQCSESTFFQECQRKLEHKLGLDSYLLKPVQRLTKYQLLLKELLKYSTSCDGVQELQEALVAMLDLLKSVNDSMHQISITGYDGDLSELGKVLMQGSFSVWTGHRKGPTKMKDLARFKPMQRHLFLYEKALVFCKKREDHGDGYDKTSSYSFKHFLKMNAVGITENVKGDHRKFEIWYSGREEVYVVQAQTVDLKMAWLNEIRKILFKQQELIKVEKQQPGSCTDQLHLSSQLSDGKQQRASISSEENDSERTSPVPLDSVLLSPQSKPHRSWPGMSQSLEICEGLEEWSGNQYLSNCSDTEEEEGNQLSPGKYKALADCKRRGSEDLLVKNGDEIQLLHEDGEGQWLVKNLNRRKEGWIPVHSLQIVVGDCRFRNAKVADAALCNTRKFSSP

Target ORF information:

RefSeq Version	XM_015278485.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015278485.1

ORF Insert Sequence:

ATGGCCGAGG CGAGCCCCGC GCGGGGCGTC AGGAGGCCGC GGAGGGATGC GGCGTCATTC 
CCAGGGAACC TGCACTTGGT GATGGTTCTT CGTCCCACAG GGTTTTTCCA ACGCACCTTC 
ACTGACATTG GATTTCGATT TAGCCAGGAG GATTTTCTGC TCAAGTTACC GGTTGTTATG 
CTGAGCTCAG TTAGCGACCT GCTGACATAC ATTGATGAGC AGCAGCTAAC CCCAGAGTTT 
GGAGGCACCT TGGAGTACTG CCACAGCGAG TGGGTCATCT TCAGGACTGC AATAGAGAGT 
TTTGCACTAA CCGTGAAGGA AATTGCACAG ATGTTACAAT CTTTTGGCAC AGAGCTGGCA 
GAGACTGAGC TACCAGAGGA TATGTATTCC ATTGAGCGCA TCCTGGCCCT GCGCACTGAG 
AAGTACTTCC AGCTCAAGGA AGATATTACA GCAGTCACAA AAGAAGGAAA GTTGCTTTTA 
AGCAATTTAG AAGATCCTGA TGTGGGTGAA TGCAGCCAGG ACCAGCACCG AGAGAGACCT 
GGAGACTGGG AGACTGTGAA TAGGCTACTC GGGCAGCTGC GGGAGATGGA GACTGCTTTT 
GATGGCTTCT GGGAAAAGCA TCAGTTGAAA ATGGAGCAGT ATTTACAACT GTGGAAGTTT 
GAGCAGAGTT TTCAAGAGGT CAAGAATGCC ATTGAATTTT TAATGGGTCA ACAAGCAAAA 
CTGCCTGACA CTGGAGACAG TGTCTCCCAA GTGAAACAGA GGCTGAAGGA CTTGAGTCAT 
TTTGATGGTA TGGCTCAGGA TCTGATAGGG AAGGCTCAGG TGGTGATACT ACACGGACAC 
CAGCTAGCAG CAAATCATCA CTATGCGCTC AACTTAATCT GCCAGCAGTG CAACGAGCTG 
CGGCACCATT CTGATGTCTT GTCTGACGAG ATCAAAAGGA AGCAGATGCG GCTGCAGAAG 
ACCTTGGATC TTCATACTCG CCTTCAGCAG GCCCTGCAAT GCTGTGATGA GGGTGCCTAC 
CTGCTTGCCA ACCAGCAGAT GGACAAGTGC CAGTCTAAAG AAGGTGCTCA GAAAGCTCTC 
CAGGACATAG AAAGATTTCT TGAAAGCTCT TCGCCCTACT TAAACTATGA TCCCCAATCC 
CTACAATATG ATTTTGAGTC AGTCTTAACA GCTGAATTGA AGTGCCAGAT ACAGTCAGTG 
CAGGTGAAGC TTGAAAATGT TCGAAGCATG TTTGAGAACC GTCAGTCTTG CTTCAAGAAG 
CTGTTGGATA AGCATGTGCG TCCCGTCCAG TTAGTGGCCC CTCGGCCAGA AAACCCACCA 
CGATCCAAGT CACCATTATT CTCTCCTAAA CATGGTGTGG ATTTTAATTC CAGTTTGAAA 
TTTTCATTTG ATCTCTCTCT CCCTGGGAAG AAAACATCAA GGAAAACTCC AAGTTCTCGC 
AAGATCGAAG TAATGCACGA TTATCAGGAA AAACGGAATT CCTTGCAGTC TTTTATTTCA 
GACAGTGATG ACAACTTGGA TATACTGAAA GGCCACGTCA TAAATGAGCT TATAGAAACT 
GAGAGAGTGT ACGTAGAGGA ACTGTTCACT GTTCTGACGG GCTACAGAGC TGAGATGGAC 
AACCCTGCCA TGGTCATCCT TATGCCTCCC GTGCTGAGGA ACAGGAAGGA TGTCCTCTTT 
GGAAACATGC CTGAGATATA TGACTTTCAC AACAAAATAT TCCTGCACAG TTTGGAAAGC 
TGCCTGGGAG CACCCGAGAG AGTGGGATTT TGTTTCCTAG ACAGGCGAGA GGATTTCCAG 
ATGTATGAGA AATACTGTCA GAACAAGCCC CGCTCAGAGT CCCTGTGGAG GCAGTGCTCG 
GAGAGCACCT TCTTCCAGGA GTGCCAAAGA AAATTAGAGC ACAAACTCGG GCTGGATTCC 
TATTTGCTCA AACCAGTGCA GCGCCTGACA AAATACCAGT TGCTTCTGAA GGAGTTGTTA 
AAGTACAGCA CAAGCTGTGA TGGAGTTCAG GAACTTCAAG AAGCTCTGGT TGCAATGCTG 
GATTTGCTAA AATCAGTCAA TGACTCTATG CATCAGATTT CAATAACAGG ATATGACGGC 
GACTTGAGTG AACTAGGAAA AGTATTGATG CAAGGATCTT TCAGCGTTTG GACAGGACAC 
CGAAAAGGTC CAACAAAAAT GAAGGATCTT GCCAGGTTCA AGCCAATGCA GAGGCATCTC 
TTCCTGTATG AGAAAGCCTT GGTCTTCTGC AAGAAGCGAG AGGATCATGG AGATGGATAT 
GATAAAACTT CATCTTACAG TTTTAAGCAT TTTCTGAAAA TGAATGCAGT TGGAATAACT 
GAGAACGTGA AGGGGGATCA TAGGAAATTT GAAATCTGGT ATAGTGGTCG AGAAGAGGTG 
TATGTTGTGC AGGCCCAAAC AGTAGATCTG AAGATGGCAT GGTTGAATGA AATCAGAAAA 
ATTTTATTCA AGCAGCAGGA GCTCATAAAA GTGGAGAAGC AGCAGCCTGG CTCATGCACT 
GACCAGCTCC ACCTCTCCTC CCAGCTGAGC GATGGAAAGC AGCAGAGAGC CTCCATAAGC 
TCAGAAGAGA ACGACTCAGA GCGCACCAGC CCGGTACCGT TGGACAGCGT GCTCCTGTCA 
CCCCAGAGCA AACCCCACAG AAGCTGGCCA GGAATGTCAC AGTCCCTGGA GATCTGTGAG 
GGGCTGGAGG AGTGGTCGGG TAACCAGTAC CTTTCCAACT GCTCGGACAC CGAAGAGGAA 
GAGGGGAACC AGCTGTCTCC AGGAAAATAC AAAGCCCTTG CAGACTGCAA GAGGAGAGGA 
TCAGAGGATC TGCTGGTGAA AAATGGGGAT GAAATTCAGC TTTTGCATGA AGATGGCGAG 
GGACAGTGGT TGGTGAAAAA CCTAAACAGA AGAAAAGAAG GCTGGATCCC TGTCCACAGT 
CTGCAGATAG TAGTCGGTGA TTGTAGATTT CGGAATGCCA AAGTTGCAGA TGCTGCCCTG 
TGTAACACGC GAAAGTTTAG TTCCCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2 ( XM_015278485.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015133971.1 Gallus gallus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X4, mRNA.

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CloneID	OGa34764
Clone ID Related Accession (Same CDS sequence)	XM_015278485.1 , XM_015278485.2
Accession Version	XM_015278485.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	proto-oncogene DBL isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015278485.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CGAGCCCCGC GCGGGGCGTC AGGAGGCCGC GGAGGGATGC GGCGTCATTC 
CCAGGGAACC TGCACTTGGT GATGGTTCTT CGTCCCACAG GGTTTTTCCA ACGCACCTTC 
ACTGACATTG GATTTCGATT TAGCCAGGAG GATTTTCTGC TCAAGTTACC GGTTGTTATG 
CTGAGCTCAG TTAGCGACCT GCTGACATAC ATTGATGAGC AGCAGCTAAC CCCAGAGTTT 
GGAGGCACCT TGGAGTACTG CCACAGCGAG TGGGTCATCT TCAGGACTGC AATAGAGAGT 
TTTGCACTAA CCGTGAAGGA AATTGCACAG ATGTTACAAT CTTTTGGCAC AGAGCTGGCA 
GAGACTGAGC TACCAGAGGA TATGTATTCC ATTGAGCGCA TCCTGGCCCT GCGCACTGAG 
AAGTACTTCC AGCTCAAGGA AGATATTACA GCAGTCACAA AAGAAGGAAA GTTGCTTTTA 
AGCAATTTAG AAGATCCTGA TGTGGGTGAA TGCAGCCAGG ACCAGCACCG AGAGAGACCT 
GGAGACTGGG AGACTGTGAA TAGGCTACTC GGGCAGCTGC GGGAGATGGA GACTGCTTTT 
GATGGCTTCT GGGAAAAGCA TCAGTTGAAA ATGGAGCAGT ATTTACAACT GTGGAAGTTT 
GAGCAGAGTT TTCAAGAGGT CAAGAATGCC ATTGAATTTT TAATGGGTCA ACAAGCAAAA 
CTGCCTGACA CTGGAGACAG TGTCTCCCAA GTGAAACAGA GGCTGAAGGA CTTGAGTCAT 
TTTGATGGTA TGGCTCAGGA TCTGATAGGG AAGGCTCAGG TGGTGATACT ACACGGACAC 
CAGCTAGCAG CAAATCATCA CTATGCGCTC AACTTAATCT GCCAGCAGTG CAACGAGCTG 
CGGCACCATT CTGATGTCTT GTCTGACGAG ATCAAAAGGA AGCAGATGCG GCTGCAGAAG 
ACCTTGGATC TTCATACTCG CCTTCAGCAG GCCCTGCAAT GCTGTGATGA GGGTGCCTAC 
CTGCTTGCCA ACCAGCAGAT GGACAAGTGC CAGTCTAAAG AAGGTGCTCA GAAAGCTCTC 
CAGGACATAG AAAGATTTCT TGAAAGCTCT TCGCCCTACT TAAACTATGA TCCCCAATCC 
CTACAATATG ATTTTGAGTC AGTCTTAACA GCTGAATTGA AGTGCCAGAT ACAGTCAGTG 
CAGGTGAAGC TTGAAAATGT TCGAAGCATG TTTGAGAACC GTCAGTCTTG CTTCAAGAAG 
CTGTTGGATA AGCATGTGCG TCCCGTCCAG TTAGTGGCCC CTCGGCCAGA AAACCCACCA 
CGATCCAAGT CACCATTATT CTCTCCTAAA CATGGTGTGG ATTTTAATTC CAGTTTGAAA 
TTTTCATTTG ATCTCTCTCT CCCTGGGAAG AAAACATCAA GGAAAACTCC AAGTTCTCGC 
AAGATCGAAG TAATGCACGA TTATCAGGAA AAACGGAATT CCTTGCAGTC TTTTATTTCA 
GACAGTGATG ACAACTTGGA TATACTGAAA GGCCACGTCA TAAATGAGCT TATAGAAACT 
GAGAGAGTGT ACGTAGAGGA ACTGTTCACT GTTCTGACGG GCTACAGAGC TGAGATGGAC 
AACCCTGCCA TGGTCATCCT TATGCCTCCC GTGCTGAGGA ACAGGAAGGA TGTCCTCTTT 
GGAAACATGC CTGAGATATA TGACTTTCAC AACAAAATAT TCCTGCACAG TTTGGAAAGC 
TGCCTGGGAG CACCCGAGAG AGTGGGATTT TGTTTCCTAG ACAGGCGAGA GGATTTCCAG 
ATGTATGAGA AATACTGTCA GAACAAGCCC CGCTCAGAGT CCCTGTGGAG GCAGTGCTCG 
GAGAGCACCT TCTTCCAGGA GTGCCAAAGA AAATTAGAGC ACAAACTCGG GCTGGATTCC 
TATTTGCTCA AACCAGTGCA GCGCCTGACA AAATACCAGT TGCTTCTGAA GGAGTTGTTA 
AAGTACAGCA CAAGCTGTGA TGGAGTTCAG GAACTTCAAG AAGCTCTGGT TGCAATGCTG 
GATTTGCTAA AATCAGTCAA TGACTCTATG CATCAGATTT CAATAACAGG ATATGACGGC 
GACTTGAGTG AACTAGGAAA AGTATTGATG CAAGGATCTT TCAGCGTTTG GACAGGACAC 
CGAAAAGGTC CAACAAAAAT GAAGGATCTT GCCAGGTTCA AGCCAATGCA GAGGCATCTC 
TTCCTGTATG AGAAAGCCTT GGTCTTCTGC AAGAAGCGAG AGGATCATGG AGATGGATAT 
GATAAAACTT CATCTTACAG TTTTAAGCAT TTTCTGAAAA TGAATGCAGT TGGAATAACT 
GAGAACGTGA AGGGGGATCA TAGGAAATTT GAAATCTGGT ATAGTGGTCG AGAAGAGGTG 
TATGTTGTGC AGGCCCAAAC AGTAGATCTG AAGATGGCAT GGTTGAATGA AATCAGAAAA 
ATTTTATTCA AGCAGCAGGA GCTCATAAAA GTGGAGAAGC AGCAGCCTGG CTCATGCACT 
GACCAGCTCC ACCTCTCCTC CCAGCTGAGC GATGGAAAGC AGCAGAGAGC CTCCATAAGC 
TCAGAAGAGA ACGACTCAGA GCGCACCAGC CCGGTACCGT TGGACAGCGT GCTCCTGTCA 
CCCCAGAGCA AACCCCACAG AAGCTGGCCA GGAATGTCAC AGTCCCTGGA GATCTGTGAG 
GGGCTGGAGG AGTGGTCGGG TAACCAGTAC CTTTCCAACT GCTCGGACAC CGAAGAGGAA 
GAGGGGAACC AGCTGTCTCC AGGAAAATAC AAAGCCCTTG CAGACTGCAA GAGGAGAGGA 
TCAGAGGATC TGCTGGTGAA AAATGGGGAT GAAATTCAGC TTTTGCATGA AGATGGCGAG 
GGACAGTGGT TGGTGAAAAA CCTAAACAGA AGAAAAGAAG GCTGGATCCC TGTCCACAGT 
CTGCAGATAG TAGTCGGTGA TTGTAGATTT CGGAATGCCA AAGTTGCAGA TGCTGCCCTG 
TGTAACACGC GAAAGTTTAG TTCCCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015133971.1
CDS	112..3141
Translation	MAEASPARGVRRPRRDAASFPGNLHLVMVLRPTGFFQRTFTDIGFRFSQEDFLLKLPVVMLSSVSDLLTYIDEQQLTPEFGGTLEYCHSEWVIFRTAIESFALTVKEIAQMLQSFGTELAETELPEDMYSIERILALRTEKYFQLKEDITAVTKEGKLLLSNLEDPDVGECSQDQHRERPGDWETVNRLLGQLREMETAFDGFWEKHQLKMEQYLQLWKFEQSFQEVKNAIEFLMGQQAKLPDTGDSVSQVKQRLKDLSHFDGMAQDLIGKAQVVILHGHQLAANHHYALNLICQQCNELRHHSDVLSDEIKRKQMRLQKTLDLHTRLQQALQCCDEGAYLLANQQMDKCQSKEGAQKALQDIERFLESSSPYLNYDPQSLQYDFESVLTAELKCQIQSVQVKLENVRSMFENRQSCFKKLLDKHVRPVQLVAPRPENPPRSKSPLFSPKHGVDFNSSLKFSFDLSLPGKKTSRKTPSSRKIEVMHDYQEKRNSLQSFISDSDDNLDILKGHVINELIETERVYVEELFTVLTGYRAEMDNPAMVILMPPVLRNRKDVLFGNMPEIYDFHNKIFLHSLESCLGAPERVGFCFLDRREDFQMYEKYCQNKPRSESLWRQCSESTFFQECQRKLEHKLGLDSYLLKPVQRLTKYQLLLKELLKYSTSCDGVQELQEALVAMLDLLKSVNDSMHQISITGYDGDLSELGKVLMQGSFSVWTGHRKGPTKMKDLARFKPMQRHLFLYEKALVFCKKREDHGDGYDKTSSYSFKHFLKMNAVGITENVKGDHRKFEIWYSGREEVYVVQAQTVDLKMAWLNEIRKILFKQQELIKVEKQQPGSCTDQLHLSSQLSDGKQQRASISSEENDSERTSPVPLDSVLLSPQSKPHRSWPGMSQSLEICEGLEEWSGNQYLSNCSDTEEEEGNQLSPGKYKALADCKRRGSEDLLVKNGDEIQLLHEDGEGQWLVKNLNRRKEGWIPVHSLQIVVGDCRFRNAKVADAALCNTRKFSSP

Target ORF information:

RefSeq Version	XM_015278485.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015278485.2

ORF Insert Sequence:

ATGGCCGAGG CGAGCCCCGC GCGGGGCGTC AGGAGGCCGC GGAGGGATGC GGCGTCATTC 
CCAGGGAACC TGCACTTGGT GATGGTTCTT CGTCCCACAG GGTTTTTCCA ACGCACCTTC 
ACTGACATTG GATTTCGATT TAGCCAGGAG GATTTTCTGC TCAAGTTACC GGTTGTTATG 
CTGAGCTCAG TTAGCGACCT GCTGACATAC ATTGATGAGC AGCAGCTAAC CCCAGAGTTT 
GGAGGCACCT TGGAGTACTG CCACAGCGAG TGGGTCATCT TCAGGACTGC AATAGAGAGT 
TTTGCACTAA CCGTGAAGGA AATTGCACAG ATGTTACAAT CTTTTGGCAC AGAGCTGGCA 
GAGACTGAGC TACCAGAGGA TATGTATTCC ATTGAGCGCA TCCTGGCCCT GCGCACTGAG 
AAGTACTTCC AGCTCAAGGA AGATATTACA GCAGTCACAA AAGAAGGAAA GTTGCTTTTA 
AGCAATTTAG AAGATCCTGA TGTGGGTGAA TGCAGCCAGG ACCAGCACCG AGAGAGACCT 
GGAGACTGGG AGACTGTGAA TAGGCTACTC GGGCAGCTGC GGGAGATGGA GACTGCTTTT 
GATGGCTTCT GGGAAAAGCA TCAGTTGAAA ATGGAGCAGT ATTTACAACT GTGGAAGTTT 
GAGCAGAGTT TTCAAGAGGT CAAGAATGCC ATTGAATTTT TAATGGGTCA ACAAGCAAAA 
CTGCCTGACA CTGGAGACAG TGTCTCCCAA GTGAAACAGA GGCTGAAGGA CTTGAGTCAT 
TTTGATGGTA TGGCTCAGGA TCTGATAGGG AAGGCTCAGG TGGTGATACT ACACGGACAC 
CAGCTAGCAG CAAATCATCA CTATGCGCTC AACTTAATCT GCCAGCAGTG CAACGAGCTG 
CGGCACCATT CTGATGTCTT GTCTGACGAG ATCAAAAGGA AGCAGATGCG GCTGCAGAAG 
ACCTTGGATC TTCATACTCG CCTTCAGCAG GCCCTGCAAT GCTGTGATGA GGGTGCCTAC 
CTGCTTGCCA ACCAGCAGAT GGACAAGTGC CAGTCTAAAG AAGGTGCTCA GAAAGCTCTC 
CAGGACATAG AAAGATTTCT TGAAAGCTCT TCGCCCTACT TAAACTATGA TCCCCAATCC 
CTACAATATG ATTTTGAGTC AGTCTTAACA GCTGAATTGA AGTGCCAGAT ACAGTCAGTG 
CAGGTGAAGC TTGAAAATGT TCGAAGCATG TTTGAGAACC GTCAGTCTTG CTTCAAGAAG 
CTGTTGGATA AGCATGTGCG TCCCGTCCAG TTAGTGGCCC CTCGGCCAGA AAACCCACCA 
CGATCCAAGT CACCATTATT CTCTCCTAAA CATGGTGTGG ATTTTAATTC CAGTTTGAAA 
TTTTCATTTG ATCTCTCTCT CCCTGGGAAG AAAACATCAA GGAAAACTCC AAGTTCTCGC 
AAGATCGAAG TAATGCACGA TTATCAGGAA AAACGGAATT CCTTGCAGTC TTTTATTTCA 
GACAGTGATG ACAACTTGGA TATACTGAAA GGCCACGTCA TAAATGAGCT TATAGAAACT 
GAGAGAGTGT ACGTAGAGGA ACTGTTCACT GTTCTGACGG GCTACAGAGC TGAGATGGAC 
AACCCTGCCA TGGTCATCCT TATGCCTCCC GTGCTGAGGA ACAGGAAGGA TGTCCTCTTT 
GGAAACATGC CTGAGATATA TGACTTTCAC AACAAAATAT TCCTGCACAG TTTGGAAAGC 
TGCCTGGGAG CACCCGAGAG AGTGGGATTT TGTTTCCTAG ACAGGCGAGA GGATTTCCAG 
ATGTATGAGA AATACTGTCA GAACAAGCCC CGCTCAGAGT CCCTGTGGAG GCAGTGCTCG 
GAGAGCACCT TCTTCCAGGA GTGCCAAAGA AAATTAGAGC ACAAACTCGG GCTGGATTCC 
TATTTGCTCA AACCAGTGCA GCGCCTGACA AAATACCAGT TGCTTCTGAA GGAGTTGTTA 
AAGTACAGCA CAAGCTGTGA TGGAGTTCAG GAACTTCAAG AAGCTCTGGT TGCAATGCTG 
GATTTGCTAA AATCAGTCAA TGACTCTATG CATCAGATTT CAATAACAGG ATATGACGGC 
GACTTGAGTG AACTAGGAAA AGTATTGATG CAAGGATCTT TCAGCGTTTG GACAGGACAC 
CGAAAAGGTC CAACAAAAAT GAAGGATCTT GCCAGGTTCA AGCCAATGCA GAGGCATCTC 
TTCCTGTATG AGAAAGCCTT GGTCTTCTGC AAGAAGCGAG AGGATCATGG AGATGGATAT 
GATAAAACTT CATCTTACAG TTTTAAGCAT TTTCTGAAAA TGAATGCAGT TGGAATAACT 
GAGAACGTGA AGGGGGATCA TAGGAAATTT GAAATCTGGT ATAGTGGTCG AGAAGAGGTG 
TATGTTGTGC AGGCCCAAAC AGTAGATCTG AAGATGGCAT GGTTGAATGA AATCAGAAAA 
ATTTTATTCA AGCAGCAGGA GCTCATAAAA GTGGAGAAGC AGCAGCCTGG CTCATGCACT 
GACCAGCTCC ACCTCTCCTC CCAGCTGAGC GATGGAAAGC AGCAGAGAGC CTCCATAAGC 
TCAGAAGAGA ACGACTCAGA GCGCACCAGC CCGGTACCGT TGGACAGCGT GCTCCTGTCA 
CCCCAGAGCA AACCCCACAG AAGCTGGCCA GGAATGTCAC AGTCCCTGGA GATCTGTGAG 
GGGCTGGAGG AGTGGTCGGG TAACCAGTAC CTTTCCAACT GCTCGGACAC CGAAGAGGAA 
GAGGGGAACC AGCTGTCTCC AGGAAAATAC AAAGCCCTTG CAGACTGCAA GAGGAGAGGA 
TCAGAGGATC TGCTGGTGAA AAATGGGGAT GAAATTCAGC TTTTGCATGA AGATGGCGAG 
GGACAGTGGT TGGTGAAAAA CCTAAACAGA AGAAAAGAAG GCTGGATCCC TGTCCACAGT 
CTGCAGATAG TAGTCGGTGA TTGTAGATTT CGGAATGCCA AAGTTGCAGA TGCTGCCCTG 
TGTAACACGC GAAAGTTTAG TTCCCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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MCF2 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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