DIO1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	DIO1
Entrez Gene ID	395940
Full Name	iodothyronine deiodinase 1
General protein information	Preferred Names iodothyronine deiodinase 1 Names type I iodothyronine deiodinase 5DI DIOI deiodinase, iodothyronine type I type 1 DI type-I 5'-deiodinase
Gene Type	protein-coding
Organism	Gallus gallus(chicken)
Summary	The protein encoded by this gene belongs to the iodothyronine deiodinase family. It catalyzes the activation, as well as the inactivation of thyroid hormone by outer and inner ring deiodination, respectively. The activation reaction involves the conversion of the prohormone thyroxine (3,5,3',5'-tetraiodothyronine, T4), secreted by the thyroid gland, to the bioactive thyroid hormone (3,5,3'-triiodothyronine, T3) by 5'-deiodination. This protein is expressed predominantly in the liver and kidney and provides most of the circulating T3, which is essential for growth, differentiation and basal metabolism in vertebrates. This protein is a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. [provided by RefSeq, Apr 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001097614.1	NP_001091083.1	type I iodothyronine deiodinase

Pathways from BioSystems

Homologies

Bos taurus (cattle)	DIO1	NP_001116065.1
Rattus norvegicus (Norway rat)	Dio1	NP_067685.5
Gallus gallus (chicken)	DIO1	NP_001091083.1
Danio rerio (zebrafish)	dio1	NP_001007284.2
Xenopus tropicalis (tropical clawed frog)	dio1	NP_001243226.1
Homo sapiens (human)	DIO1	NP_000783.2
Canis lupus familiaris (dog)	DIO1	NP_001007127.1
	DIO1	NP_001116124.1
Mus musculus (house mouse)	Dio1	NP_031886.3
Pan troglodytes (chimpanzee)	DIO1	NP_001116123.1

Gene Ontology
Bibliography

Related articles in PubMed

ESTs from brain and testis of White Leghorn and red junglefowl: annotation, bioinformatic classification of unknown transcripts and analysis of expression levels.
Savolainen P, Fitzsimmons C, Arvestad L, Andersson L, Lundeberg J
Cytogenetic and genome research111(1)79-87(2005)

Type I iodothyronine deiodinase in euthyroid and hypothyroid chicken cerebellum.
Verhoelst CH, Darras VM, Doulabi BZ, Reyns G, K?hn ER, Van der Geyten S
Molecular and cellular endocrinology214(1-2)97-105(2004 Feb)

A comprehensive collection of chicken cDNAs.
Boardman PE, Sanz-Ezquerro J, Overton IM, Burt DW, Bosch E, Fong WT, Tickle C, Brown WR, Wilson SA, Hubbard SJ
Current biology : CB12(22)1965-9(2002 Nov)

Expression of chicken hepatic type I and type III iodothyronine deiodinases during embryonic development.
Van der Geyten S, Sanders JP, Kaptein E, Darras VM, K?hn ER, Leonard JL, Visser TJ
Endocrinology138(12)5144-52(1997 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

deiodinase localizes in the internal granule cells of the cerebellum in day-old chicks; migration of immature granule cells from the external towards the internal layer was completed at this stage while cell migration was retarded in hypothyroid animals.
Title: Type I iodothyronine deiodinase in euthyroid and hypothyroid chicken cerebellum.

The following DIO1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DIO1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa27989	NM_001097614.1 Latest version!	Gallus gallus deiodinase, iodothyronine type I (DIO1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DIO1 ( NM_001097614.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001091083.1 Gallus gallus deiodinase, iodothyronine type I (DIO1), mRNA.

Price & Availability↑

CloneID	OGa27989
Clone ID Related Accession (Same CDS sequence)	NM_001097614.1
Accession Version	NM_001097614.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Gallus gallus(chicken)
Product	type I iodothyronine deiodinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU243761.1, Y11110.1 and CN224495.1. On May 25, 2007 this sequence version replaced XM_422487.2. ##Evidence-Data-START## Transcript exon combination :: Y11110.1, BU382680.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: PMID: 9389494 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTGAGCA TCAGGGTGCT CCTACACAAA CTCCTGATTC TTTTGCAGGT TACTCTGAGT 
GTTGTCGTTG GTAAAACCAT GATGATACTC TTCCCCGACA CCACGAAAAG ATACATCCTA 
AAGCTGGGCG AAAAGAGCAG AATGAACCAG AACCCAAAGT TCAGCTACGA AAACTGGGGT 
CCCACTTTTT TCAGCTTCCA GTATTTGCTC TTTGTGCTGA AGGTGAAGTG GAGGAGGCTG 
GAAGACGAAG CCCACGAGGG ACGCCCTGCC CCCAACACAC CGGTGGTGGC TCTGAATGGG 
GAGATGCAGC ACCTCTTCAG TTTCATGCGA GATAACCGAC CTTTAATCCT CAATTTTGGA 
AGCTGCACCT GACCTTCATT TATGTTAAAA TTTGATGAGT TCAACAAACT TGTCAAAGAT 
TTCAGCTCTA TAGCAGATTT CCTTATCATC TACATCGAAG AAGCTCACGC AGTAGATGGA 
TGGGCCTTCA GAAACAATGT TGTTATTAAA AATCACAGAA GCCTTGAGGA TCGAAAAACT 
GCAGCACAAT TTCTTCAGCA GAAGAACCCC TTATGTCCAG TGGTTTTAGA CACAATGGAA 
AACCTGAGCA GCTCAAAATA CGCAGCGCTG CCAGAAAGAC TGTATATACT TCAAGCAGGG 
AATGTCATCT ACAAGGGAGG AGTGGGGCCT TGGAATTACC ACCCCCAGGA AATACGCGCT 
GTCCTGGAAA AACTGAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001091083.1
CDS	3..743
Translation	MLSIRVLLHKLLILLQVTLSVVVGKTMMILFPDTTKRYILKLGEKSRMNQNPKFSYENWGPTFFSFQYLLFVLKVKWRRLEDEAHEGRPAPNTPVVALNGEMQHLFSFMRDNRPLILNFGSCT*PSFMLKFDEFNKLVKDFSSIADFLIIYIEEAHAVDGWAFRNNVVIKNHRSLEDRKTAAQFLQQKNPLCPVVLDTMENLSSSKYAALPERLYILQAGNVIYKGGVGPWNYHPQEIRAVLEKLK

Target ORF information:

RefSeq Version	NM_001097614.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus deiodinase, iodothyronine type I (DIO1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001097614.1

ORF Insert Sequence:

ATGTTGAGCA TCAGGGTGCT CCTACACAAA CTCCTGATTC TTTTGCAGGT TACTCTGAGT 
GTTGTCGTTG GTAAAACCAT GATGATACTC TTCCCCGACA CCACGAAAAG ATACATCCTA 
AAGCTGGGCG AAAAGAGCAG AATGAACCAG AACCCAAAGT TCAGCTACGA AAACTGGGGT 
CCCACTTTTT TCAGCTTCCA GTATTTGCTC TTTGTGCTGA AGGTGAAGTG GAGGAGGCTG 
GAAGACGAAG CCCACGAGGG ACGCCCTGCC CCCAACACAC CGGTGGTGGC TCTGAATGGG 
GAGATGCAGC ACCTCTTCAG TTTCATGCGA GATAACCGAC CTTTAATCCT CAATTTTGGA 
AGCTGCACCT GACCTTCATT TATGTTAAAA TTTGATGAGT TCAACAAACT TGTCAAAGAT 
TTCAGCTCTA TAGCAGATTT CCTTATCATC TACATCGAAG AAGCTCACGC AGTAGATGGA 
TGGGCCTTCA GAAACAATGT TGTTATTAAA AATCACAGAA GCCTTGAGGA TCGAAAAACT 
GCAGCACAAT TTCTTCAGCA GAAGAACCCC TTATGTCCAG TGGTTTTAGA CACAATGGAA 
AACCTGAGCA GCTCAAAATA CGCAGCGCTG CCAGAAAGAC TGTATATACT TCAAGCAGGG 
AATGTCATCT ACAAGGGAGG AGTGGGGCCT TGGAATTACC ACCCCCAGGA AATACGCGCT 
GTCCTGGAAA AACTGAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ESTs from brain and testis of White Leghorn and red junglefowl: annotation, bioinformatic classification of unknown transcripts and analysis of expression levels.
Cytogenetic and genome research111(1)79-87(2005)
Savolainen P,Fitzsimmons C,Arvestad L,Andersson L,Lundeberg J

Type I iodothyronine deiodinase in euthyroid and hypothyroid chicken cerebellum.
Molecular and cellular endocrinology214(1-2)97-105(2004 Feb)
Verhoelst CH,Darras VM,Doulabi BZ,Reyns G,K?hn ER,Van der Geyten S

A comprehensive collection of chicken cDNAs.
Current biology : CB12(22)1965-9(2002 Nov)
Boardman PE,Sanz-Ezquerro J,Overton IM,Burt DW,Bosch E,Fong WT,Tickle C,Brown WR,Wilson SA,Hubbard SJ

Expression of chicken hepatic type I and type III iodothyronine deiodinases during embryonic development.
Endocrinology138(12)5144-52(1997 Dec)
Van der Geyten S,Sanders JP,Kaptein E,Darras VM,K?hn ER,Leonard JL,Visser TJ

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DIO1 cDNA ORF clone, Gallus gallus(chicken)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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