ACAN cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	ACAN
Entrez Gene ID	395798
Full Name	aggrecan
Synonyms	AGC1
General protein information	Preferred Names aggrecan Names aggrecan core protein CSPCP aggrecan 1 aggrecan chondroitin sulfate CS2 domain cartilage-specific proteoglycan core protein chondroitin sulfate proteoglycan core protein proteoglycan core protein
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015291979.2	XP_015147465.1	aggrecan core protein isoform X2
XM_015291977.1	XP_015147463.1	aggrecan core protein isoform X1
XM_015291979.1	XP_015147465.1	aggrecan core protein isoform X2
XM_015291977.2	XP_015147463.1	aggrecan core protein isoform X1
XM_015291978.2	XP_015147464.1	aggrecan core protein isoform X1
NM_204955.2	NP_990286.2	aggrecan core protein precursor
XM_015291978.1	XP_015147464.1	aggrecan core protein isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	ACAN	NP_990286.2
Canis lupus familiaris (dog)	ACAN	NP_001106926.1

Gene Ontology
Bibliography

Related articles in PubMed

Hyaluronan binding to link module of TSG-6 and to G1 domain of aggrecan is differently regulated by pH.
Heng BC, Gribbon PM, Day AJ, Hardingham TE
The Journal of biological chemistry283(47)32294-301(2008 Nov)

Mutation analysis of the aggrecan gene in chickens with tibial dyschondroplasia.
Ray SA, Drummond PB, Shi L, McDaniel GR, Smith EJ
Poultry science85(7)1169-72(2006 Jul)

Differential versican isoforms and aggrecan expression in the chicken embryo aorta.
Arciniegas E, Neves CY, Candelle D, Parada D
The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology279(1)592-600(2004 Jul)

S103L reactive chondroitin sulfate proteoglycan (aggrecan) mRNA expressed in developing chick brain and cartilage is encoded by a single gene.
Li H, Domowicz M, Hennig A, Schwartz NB
Brain research. Molecular brain research36(2)309-21(1996 Mar)

Molecular cloning of chicken aggrecan.
Chandrasekaran L, Tanzer ML
The Biochemical journal296 ( Pt 3)885-7(1993 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TSG-6 and aggrecan interact with HA through structurally homologous domains, a process that is differentially regulated by pH
Title: Hyaluronan binding to link module of TSG-6 and to G1 domain of aggrecan is differently regulated by pH.

A scan for SNPs in the AGC1 gene of chicken lines divergently selected for tibial dyschondroplasia is reported.
Title: Mutation analysis of the aggrecan gene in chickens with tibial dyschondroplasia.

APBP-1 has the capacity to repress aggrecan expression and may be an important regulator of aggrecan gene expression
Title: APBP-1, a DNA/RNA-binding protein, interacts with the chick aggrecan regulatory region.

Immunolocalization patterns observed for versican may be related to the predominance of the V2 isoform during intimal thickening formation and endothelial-mesenchymal transformation.
Title: Differential versican isoforms and aggrecan expression in the chicken embryo aorta.

The following ACAN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACAN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa28230	NM_204955.2 Latest version!	Gallus gallus aggrecan (ACAN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OGa28230	XM_015291978.2 Latest version!	Gallus gallus aggrecan (ACAN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OGa28230	XM_015291977.2 Latest version!	Gallus gallus aggrecan (ACAN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OGa40533	XM_015291979.2 Latest version!	Gallus gallus aggrecan (ACAN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
View Old Accession Versions >>
OGa40533	XM_015291979.1	Gallus gallus aggrecan (ACAN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OGa28230	XM_015291977.1	Gallus gallus aggrecan (ACAN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OGa28230	XM_015291978.1	Gallus gallus aggrecan (ACAN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ACAN ( NM_204955.2 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_990286.2 Gallus gallus aggrecan (ACAN), mRNA.

Price & Availability↑

CloneID	OGa28230
Clone ID Related Accession (Same CDS sequence)	XM_015291977.1 , XM_015291977.2 , XM_015291978.2 , NM_204955.2 , XM_015291978.1
Accession Version	NM_204955.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Gallus gallus(chicken)
Product	aggrecan core protein precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from U78555.1 and AADN05000047.1. On or before Jan 15, 2012 this sequence version replaced XM_001232949.2, NM_204955.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data from different strains to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2201357, SAMEA2201358 [ECO:0000350] ##Evidence-Data-END##

ATGACCACTC TACTACTAGT GTTTGTGTGT TTGCAAGCCA TCACCACAGC TGCCTCCGCA 
GAGCTCTCAG ACAGTAGCGA TGGCCTGGAA GTGAAGATAC CTGAGCAGTC TCCCCTGCGT 
GTTGTCCTGG GAAGCTCCCT GAACATCCCC TGCTATTTCA ACATCCCAGA GGAAGAGGAC 
ACCAATGCTC TGCTGACCCC CCGGATCAAA TGGAGCAAGC TTTCCAATGG AACAGAGATT 
GTCTTACTAG TGGCCACCGG TGGGAAGATC CGGCTCAATG CAGAGTACAG AGAGGTGATC 
TCCTTGCCCA ATTACCCTGC CATCCCCACT GATGCCACCT TGGAAATCAA GGCGCTGAGA 
TCCAACCACA CTGGGATTTA TCGCTGTGAA GTGATGTATG GGATTGAGGA CAGACAAGAC 
ACCATAGAGG TCCTGGTGAA AGGCGTCGTG TTCCACTACA GAGCAATCTC CACAAGGTAC 
ACTTTGAACT TCGAGAGGGC AAAGCAGGCC TGTATCCAGA ACAGTGCTGT CATTGCCACC 
CCTGAGCAGC TGCAGGCTGC CTACGAGGAT GGGTACGAGC AGTGCGATGC CGGCTGGCTG 
GCTGATCAGA CTGTCAGGTA CCCCATCCAT CTGCCCCGGG AGCGCTGCTA CGGTGACAAG 
GATGAGTTTC CAGGAGTGAG AACCTACGGT GTCCGTGAGA CAGATGAAAC CTATGATGTT 
TACTGCTATG CAGAGCAAAT GCAAGGCAAA GTCTTCTACG CCACCTCCCC CGAGAAGTTC 
ACCTTCCAGG AAGCTTTTGA CAAATGCCAC AGCTTGGGAG CCCGCCTGGC CACCACGGGC 
GAGCTGTACT TGGCCTGGAA GGATGGCATG GACATGTGCA GTGCGGGCTG GCTGGCTGAC 
CGCAGCGTTC GCTACCCCAT TTCCAGAGCA CGGCCCAATT GTGGAGGGAA CCTGGTGGGC 
GTGCGGACCG TTTACCTGAA CCCTGCCAAC CAGACAGGGT ACCCTCACCC CAGCTCACGC 
TACGATGCCA TCTGTTACAG TGGTGATGAC TTCGAGGCTC TGGTCCCAGG GCTGTTCACT 
GACGAGGTTG GGACTGAGCT GGGCAGTGCT TTTACCATAC AGACCGTCAC ACAGACTGAG 
GTGGAGCTGC CCCTGCCACG CAATGTCACA GAAGAGGAGG CCCGTGGCAG CATCGCTACC 
CTGGAGCCCA TGGAGATCAC AGCCACCGCC ACTGAGCTGT ATGAGGCCTT CACCGTCCTG 
CCTGACCTCT TTGCCACCAG TGTCACAGTA GAGACAGCTT CCCCAAGGGA AGAGAACGTG 
ACCAGAGAGG AGATCACAGG GATATGGGCT GTGCCTGAAG AGGTCACTAC ATCGGTCTCA 
GGCACTGCTT TCACCACCGG AATGGCAGAG GTGAGCTCAG TGGAAGAGGC CATAGCAGTG 
ACTGCCACAC CAGGACTGGA GTCTGCCTCT CCATTCACCA TAGAAGATCA TCTCGTGCAA 
GTGACAGCAG CCCCTGATGT TGCCCTCCTC CCCAGGCAGC CCATTTCCCC CACTGGTGTG 
GTGTTCCACT ACCGTGCTGC CACCAGCAGA TACGCCTTCT CCTTCATCCA AGCCCAGCAG 
GCCTGCCTGG AGAACAATGC CGTTATTGCC ACTCCCGAGC AGCTCCAGGC TGCCTACGAG 
GCTGGCTTTG ATCAGTGCGA TGCCGGCTGG CTGCGGGACC AGACGGTCAG GTATCCCATT 
GTGAATCCCC GCAGCAACTG TGTAGGAGAC AAAGAGAGCT CCCCAGGTGT GCGGTCATAC 
GGCATGCGCC CGGCCTCAGA GACCTACGAT GTGTACTGTT ACATCGACAG GCTAAAGGGT 
GAGGTGTTTT TTGCCACCCA GCCAGAGCAG TTCACCTTCC AAGAAGCCCA GCTGTACTGT 
GAAAGCCAAA ATGCCACGCT GGCCTCTGCT GGGCAACTCC ACGCTGCCTG GAAGCAGGGC 
CTGGATAGGT GCTACCCTGG GTGGCTGGCC GACGGCAGCT TGCGGTACCC CATTGTGAGC 
CCCCGACCTG CCTGCGGGGG GGATGCACCT GGTGTGAGGA CCATCTACCA GCACCACAAC 
CAGACGGGCT TTCCTGACCC TCTGTCACGG CATCACGCTT TCTGCTTCAG AGCTCTGCCA 
TCCGTAGTGG AGGAGGGGGT GACCTCACTC TTTGAAGAAG AGGTGATGGT AACCCAACTG 
ATCCCTGGAG TGGAAGGAAT ACCTTCTGGG GAGGAAACAA CTGTGGAGAC AGAGCTTTCC 
TCTGAGCCTG AGAATCAGAC AGCCCAGGGA ACGGAGGTCT TCCCAACTGA CGTATCACTG 
CTCTCAGCGA GACCATCTGC TTTTCCTCCA GCTACTGTAA TACCAGAGGA AACAAGTACC 
AATGCTTCCA TCCCCGAAGT GTCCGGAGAG TTCCCTGAGT CTGGAGAGCA CCCAACCAGT 
GGCGAACCTT CAGCATCTGG AGCCCCAGAC ACGAGTGGAG AACCAACTTC CGTAGGTTTT 
GAACTGAGTG GAGAGCAATC CGGGATTGGA GAAAGTGGAT TACCATCTGT AGACCTGCAG 
AGCAGTGGCT TTGTACCTGG AGAAAGTGGC CTTCCCTCAG GGGATGTGAG CGGGTTGCCT 
TCTGGCATTG TTGATATCAG CGGCTTGCCT TCTGCAGAGG AAGAGGTAAC GGTGTCTGTT 
TCGAGGATAC CAGAAGTTAG TGGAATGCCA TCTGGAGCTG AAAGCAGTGG TCTGCATTCT 
GGATTTAGTG GAGAAATCTC TGGCACTGAG CTTATCAGTG GCCTGCCATC TGGAGAGGAA 
AGTGGGCTCG CCTCTGGTTT TCCCACCATC TCCCTCGTGG ATTCCACTTT GGTGGAGGTT 
GTAACGGCAG CACCGGGACG GCAAGAGGAG GGAAAAGGAT CAATTGGAGT CAGTGGTGAA 
GAAGAGCTGT CAGGGTTTCC GTCTGCAGAG TGGGACAGCA GTGGGGCCAG AGGGCTGCCC 
TCGGGAGCTG AAACCAGTGG GGAGCAATCC GGGGTGCCCG AGCTCAGTGG GGAGCATTCC 
GGGGTGCCTG GGCTCAGTGG AGAGCCTTTT GAGGTGCCTG AGCTCAGCGG GGAGCATTCC 
GGGGTGACTG AGCTCAGTGG GGAGCATTCT GGGCTTCCTG AGCTCAGTGG AGAGCCTTTT 
GGGGTGCCTG AGCTCAGCGG GTTTCCCTCC GGACTGGACA TCAGTGGGGA ACCATCTGGA 
GCACCCGAGG TGAGCGGCCC GGTGGACGTG AGCGGCCTTA CCTCTGGTGT TGATGGAAGT 
GGTGAGGTCT CAGGCGTTAC CTTTATAAGC ACCAGCCTGC AAGAAGTGAC AACCCAGTCA 
GTTGCAGAAG CAGAAGCAAA AGAAATTCTA GAGATCAGTG GACTGCCTTC AGGAGAGACA 
TCAGGCATGG TGTCTGGGAG CTTAGATGTC AGCGGTCAGC CTTCGGGACA CATAGACTTT 
GGTGGGAGCG CTTCTGGAGT GCTGGAGATG AGCGGATTTC CAAGCGGAGC GGTTGAGAGC 
AGTGGAGAAG CCTCTGGGGT TGAAGTCACC AGTGTCCTCG CGTCTGGAGA GGAAAGTGGG 
CTCACCTCAG GCTTTCCCAC CGTGTCTCTC GTGGATACCA CGTTGGTAGA AGTTGTAACG 
CAGACATCAG TTGCTCAAGA GGTGGGAGAA GGACCATCTG GGATGATAGA AATCAGTGGA 
TTTCTTTCTG GAGACAGAGG AGTATCTGGA GAAGGGTCTG GAGCTGTGCA GTCTAGTGGG 
CTTCCTTCAG GAACAGGAGA CTTCAGCGGA GAGCCATCCG GGATCCCGTA TTTCAGCGGA 
GACATTTCTG GAGCCACAGA TCTAAGCGGA CAACCTTCCG CAGTGACTGA TATTAGTGGG 
GAGGACTCGG GACTTCCAGA AGTCACTTTA GTCACATCCG ATTTAGTAGA AGTTGTGACA 
AGGCCAACAG TTTCACAGGA GCTGGGTGGG GAAACAGCTG TGACGTTTCC CTATGTCTTT 
GGGCCAAGTG GTGAGGGCTC TGCATCTGGA GACCTGAGTG GGGGAGCATC TGCAGAAGGT 
GGTATAGAAA CATCAGCAGC TTATGAAATC AGTGGTGAGA GCTCTGCCTT CCCTGAAACG 
AGTATAGAAA CATCCACAGA TCAAGAAATC AGTGGGGAAG CATCTGCATA CCCTGAGATT 
AGCGTAGAAA CATCCACGCA TCTGGAAACC AGTGGGGAAA CATCTGCATA CCCTGAGATT 
AGCACAGAGA CGTCCACCAT TCAGGAAGTT AGTGGGGAAA CGTCTGCCTT TCCTGAAATT 
AGCACAGAAA CATCCACAAT ACAAGAGATC AGTGGGGAAA CATCCGCATT TCCTGAAATT 
AGAATAGAAA CATCCACCTT TCAAGAAATC AGTGGGGAAA CGTCCGCATT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGCGGCGAAA CGTCTGCGTT TCCCGAGATT 
ACCATAGAAG CCTCCACAGT CCATGAAACC AGTGGAGAAA CATCTGCCTT TCCCGAAATT 
AGCATAGAAA CATCCACAGT CCATGAAATC AGTGGGGAAA GTTCTGCCTT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGGGGTGAAA CATCTGCGTT CCCTGAGATT 
ACCATAGAAG CCTCCACCAT TCAAGAGATC AGTGGAGAAA CATCTGCATT TCCTGAAATT 
AGCATAGCAA CTTCCACCGT CCGTGAAATC AGCGGCGAAA CGTCTGCCTT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGGGGTGAAA CATCTGCATT GCCTGAGATT 
ACCGTAGAGA CATCCACTGT CCATGAAACC AGTGGGGAAG CGTCTGCCTT TCCTGAAATT 
AGCATAGAAA CTTCCACAAG ACAAGAAGCC AGGGGTGAAG CATCCGCCTA CCCTGAGGTT 
AGCATAGAGG CATCCACAAC TCAAGAAGTA AGTGGGGAAA GTTCTGCCTT CCCTGAAATT 
AGCGTAGAGA CATCCACAAG TCAGGAAGCC CGCGGTGAAA CATCTGCCTT TCCTGAGATT 
GGCATAGAGA CATCCACAGC CCACGAAGGC AGTGGGGAAA CTCCTGGGCT GCCTGCTGTT 
AGCACTGACA CTGCTGCCAC GTCTCTGGCC AGTGGTGAGC CCTCCGGTGC TCCTGAGAAG 
GAAACTCCCG ACACAACATC ACATCTGATC ACGGGCGTTT CAGGGGAAAC CTCTGTCCCA 
GATGCTGTAA TCAGTACCAG TGCTCCAGAT GTTGAACTAG CGCAGGGACC CAGAAACACT 
GAAGAGACTC AGCTTGAAAT AGAGCCCTCC ACTCCTGCGG CATCTGGACA AGAGACAGAA 
ACAGCTGCTG TCCTCGACAA TCCCCATCTG CCAGCCACTG CTACTGCTGC CCTGCATCCA 
GCCTCCCAAG AAGCAGTAGA TGCACTGGGA CCCACGACAG AAGACACTGA TGAGTGCCAC 
TCAAGCCCCT GTCTGAATGG AGCTACCTGC GTTGATGGCA TCGACTCTTT CAAATGCTTA 
TGCCTTCCCA GCTACGGAGG GGACCTGTGT GAGATCGACC TGGCAAACTG TGAGGAAGGC 
TGGATCAAGT TCCAGGGCCA CTGCTACAGG CACTTTGAAG AGAGGGAGAC GTGGATGGAC 
GCAGAGTCCA GGTGCAGAGA ACATCAAGCC CACCTGAGCA GCATCATTAC TCCAGAGGAA 
CAAGAATTTG TGAACAGCCA TGCACAAGAC TATCAGTGGA TCGGCCTCAG TGACAGAGCC 
GTGGAGAATG ATTTCCGCTG GTCTGATGGA CACTCGCTGC AATTTGAGAA CTGGCGGCCC 
AACCAACCTG ACAACTTCTT CTCCGCGGGC GAGGACTGCG TTGTGATGAT CTGGCATGAG 
CAAGGCGAAT GGAACGACGT CCCCTGCAAC TATCACCTGC CTTTCACGTG CAAGAAGGGA 
ACAGTTGCCT GTGGGGACCC ACCTGTAGTG GAGAACGCGC GGACCTTTGG CCGGAAGAAG 
GACCGCTATG AGATCAACTC CCTGGTGCGG TACCAGTGTG ACCACGGCTA CATCCAGCGC 
CACGTGCCCA CCATCCGCTG CCAACCCAAC GGGCACTGGG AGGAACCGCG GATATCCTGC 
ACAAACCCCT CCAGCTACCA ACGCCGGCTA TACAAGAGGA GCCCCCGGAG CCGGTTGAGA 
CCCGGTGTCG TGCACAGACC CACCCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_990286.2
CDS	160..6429
Translation	MTTLLLVFVCLQAITTAASAELSDSSDGLEVKIPEQSPLRVVLGSSLNIPCYFNIPEEEDTNALLTPRIKWSKLSNGTEIVLLVATGGKIRLNAEYREVISLPNYPAIPTDATLEIKALRSNHTGIYRCEVMYGIEDRQDTIEVLVKGVVFHYRAISTRYTLNFERAKQACIQNSAVIATPEQLQAAYEDGYEQCDAGWLADQTVRYPIHLPRERCYGDKDEFPGVRTYGVRETDETYDVYCYAEQMQGKVFYATSPEKFTFQEAFDKCHSLGARLATTGELYLAWKDGMDMCSAGWLADRSVRYPISRARPNCGGNLVGVRTVYLNPANQTGYPHPSSRYDAICYSGDDFEALVPGLFTDEVGTELGSAFTIQTVTQTEVELPLPRNVTEEEARGSIATLEPMEITATATELYEAFTVLPDLFATSVTVETASPREENVTREEITGIWAVPEEVTTSVSGTAFTTGMAEVSSVEEAIAVTATPGLESASPFTIEDHLVQVTAAPDVALLPRQPISPTGVVFHYRAATSRYAFSFIQAQQACLENNAVIATPEQLQAAYEAGFDQCDAGWLRDQTVRYPIVNPRSNCVGDKESSPGVRSYGMRPASETYDVYCYIDRLKGEVFFATQPEQFTFQEAQLYCESQNATLASAGQLHAAWKQGLDRCYPGWLADGSLRYPIVSPRPACGGDAPGVRTIYQHHNQTGFPDPLSRHHAFCFRALPSVVEEGVTSLFEEEVMVTQLIPGVEGIPSGEETTVETELSSEPENQTAQGTEVFPTDVSLLSARPSAFPPATVIPEETSTNASIPEVSGEFPESGEHPTSGEPSASGAPDTSGEPTSVGFELSGEQSGIGESGLPSVDLQSSGFVPGESGLPSGDVSGLPSGIVDISGLPSAEEEVTVSVSRIPEVSGMPSGAESSGLHSGFSGEISGTELISGLPSGEESGLASGFPTISLVDSTLVEVVTAAPGRQEEGKGSIGVSGEEELSGFPSAEWDSSGARGLPSGAETSGEQSGVPELSGEHSGVPGLSGEPFEVPELSGEHSGVTELSGEHSGLPELSGEPFGVPELSGFPSGLDISGEPSGAPEVSGPVDVSGLTSGVDGSGEVSGVTFISTSLQEVTTQSVAEAEAKEILEISGLPSGETSGMVSGSLDVSGQPSGHIDFGGSASGVLEMSGFPSGAVESSGEASGVEVTSVLASGEESGLTSGFPTVSLVDTTLVEVVTQTSVAQEVGEGPSGMIEISGFLSGDRGVSGEGSGAVQSSGLPSGTGDFSGEPSGIPYFSGDISGATDLSGQPSAVTDISGEDSGLPEVTLVTSDLVEVVTRPTVSQELGGETAVTFPYVFGPSGEGSASGDLSGGASAEGGIETSAAYEISGESSAFPETSIETSTDQEISGEASAYPEISVETSTHLETSGETSAYPEISTETSTIQEVSGETSAFPEISTETSTIQEISGETSAFPEIRIETSTFQEISGETSAFPEIRIETSTSQEARGETSAFPEITIEASTVHETSGETSAFPEISIETSTVHEISGESSAFPEIRIETSTSQEARGETSAFPEITIEASTIQEISGETSAFPEISIATSTVREISGETSAFPEIRIETSTSQEARGETSALPEITVETSTVHETSGEASAFPEISIETSTRQEARGEASAYPEVSIEASTTQEVSGESSAFPEISVETSTSQEARGETSAFPEIGIETSTAHEGSGETPGLPAVSTDTAATSLASGEPSGAPEKETPDTTSHLITGVSGETSVPDAVISTSAPDVELAQGPRNTEETQLEIEPSTPAASGQETETAAVLDNPHLPATATAALHPASQEAVDALGPTTEDTDECHSSPCLNGATCVDGIDSFKCLCLPSYGGDLCEIDLANCEEGWIKFQGHCYRHFEERETWMDAESRCREHQAHLSSIITPEEQEFVNSHAQDYQWIGLSDRAVENDFRWSDGHSLQFENWRPNQPDNFFSAGEDCVVMIWHEQGEWNDVPCNYHLPFTCKKGTVACGDPPVVENARTFGRKKDRYEINSLVRYQCDHGYIQRHVPTIRCQPNGHWEEPRISCTNPSSYQRRLYKRSPRSRLRPGVVHRPTH

Target ORF information:

RefSeq Version	NM_204955.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus aggrecan (ACAN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_204955.2

ORF Insert Sequence:

ATGACCACTC TACTACTAGT GTTTGTGTGT TTGCAAGCCA TCACCACAGC TGCCTCCGCA 
GAGCTCTCAG ACAGTAGCGA TGGCCTGGAA GTGAAGATAC CTGAGCAGTC TCCCCTGCGT 
GTTGTCCTGG GAAGCTCCCT GAACATCCCC TGCTATTTCA ACATCCCAGA GGAAGAGGAC 
ACCAATGCTC TGCTGACCCC CCGGATCAAA TGGAGCAAGC TTTCCAATGG AACAGAGATT 
GTCTTACTAG TGGCCACCGG TGGGAAGATC CGGCTCAATG CAGAGTACAG AGAGGTGATC 
TCCTTGCCCA ATTACCCTGC CATCCCCACT GATGCCACCT TGGAAATCAA GGCGCTGAGA 
TCCAACCACA CTGGGATTTA TCGCTGTGAA GTGATGTATG GGATTGAGGA CAGACAAGAC 
ACCATAGAGG TCCTGGTGAA AGGCGTCGTG TTCCACTACA GAGCAATCTC CACAAGGTAC 
ACTTTGAACT TCGAGAGGGC AAAGCAGGCC TGTATCCAGA ACAGTGCTGT CATTGCCACC 
CCTGAGCAGC TGCAGGCTGC CTACGAGGAT GGGTACGAGC AGTGCGATGC CGGCTGGCTG 
GCTGATCAGA CTGTCAGGTA CCCCATCCAT CTGCCCCGGG AGCGCTGCTA CGGTGACAAG 
GATGAGTTTC CAGGAGTGAG AACCTACGGT GTCCGTGAGA CAGATGAAAC CTATGATGTT 
TACTGCTATG CAGAGCAAAT GCAAGGCAAA GTCTTCTACG CCACCTCCCC CGAGAAGTTC 
ACCTTCCAGG AAGCTTTTGA CAAATGCCAC AGCTTGGGAG CCCGCCTGGC CACCACGGGC 
GAGCTGTACT TGGCCTGGAA GGATGGCATG GACATGTGCA GTGCGGGCTG GCTGGCTGAC 
CGCAGCGTTC GCTACCCCAT TTCCAGAGCA CGGCCCAATT GTGGAGGGAA CCTGGTGGGC 
GTGCGGACCG TTTACCTGAA CCCTGCCAAC CAGACAGGGT ACCCTCACCC CAGCTCACGC 
TACGATGCCA TCTGTTACAG TGGTGATGAC TTCGAGGCTC TGGTCCCAGG GCTGTTCACT 
GACGAGGTTG GGACTGAGCT GGGCAGTGCT TTTACCATAC AGACCGTCAC ACAGACTGAG 
GTGGAGCTGC CCCTGCCACG CAATGTCACA GAAGAGGAGG CCCGTGGCAG CATCGCTACC 
CTGGAGCCCA TGGAGATCAC AGCCACCGCC ACTGAGCTGT ATGAGGCCTT CACCGTCCTG 
CCTGACCTCT TTGCCACCAG TGTCACAGTA GAGACAGCTT CCCCAAGGGA AGAGAACGTG 
ACCAGAGAGG AGATCACAGG GATATGGGCT GTGCCTGAAG AGGTCACTAC ATCGGTCTCA 
GGCACTGCTT TCACCACCGG AATGGCAGAG GTGAGCTCAG TGGAAGAGGC CATAGCAGTG 
ACTGCCACAC CAGGACTGGA GTCTGCCTCT CCATTCACCA TAGAAGATCA TCTCGTGCAA 
GTGACAGCAG CCCCTGATGT TGCCCTCCTC CCCAGGCAGC CCATTTCCCC CACTGGTGTG 
GTGTTCCACT ACCGTGCTGC CACCAGCAGA TACGCCTTCT CCTTCATCCA AGCCCAGCAG 
GCCTGCCTGG AGAACAATGC CGTTATTGCC ACTCCCGAGC AGCTCCAGGC TGCCTACGAG 
GCTGGCTTTG ATCAGTGCGA TGCCGGCTGG CTGCGGGACC AGACGGTCAG GTATCCCATT 
GTGAATCCCC GCAGCAACTG TGTAGGAGAC AAAGAGAGCT CCCCAGGTGT GCGGTCATAC 
GGCATGCGCC CGGCCTCAGA GACCTACGAT GTGTACTGTT ACATCGACAG GCTAAAGGGT 
GAGGTGTTTT TTGCCACCCA GCCAGAGCAG TTCACCTTCC AAGAAGCCCA GCTGTACTGT 
GAAAGCCAAA ATGCCACGCT GGCCTCTGCT GGGCAACTCC ACGCTGCCTG GAAGCAGGGC 
CTGGATAGGT GCTACCCTGG GTGGCTGGCC GACGGCAGCT TGCGGTACCC CATTGTGAGC 
CCCCGACCTG CCTGCGGGGG GGATGCACCT GGTGTGAGGA CCATCTACCA GCACCACAAC 
CAGACGGGCT TTCCTGACCC TCTGTCACGG CATCACGCTT TCTGCTTCAG AGCTCTGCCA 
TCCGTAGTGG AGGAGGGGGT GACCTCACTC TTTGAAGAAG AGGTGATGGT AACCCAACTG 
ATCCCTGGAG TGGAAGGAAT ACCTTCTGGG GAGGAAACAA CTGTGGAGAC AGAGCTTTCC 
TCTGAGCCTG AGAATCAGAC AGCCCAGGGA ACGGAGGTCT TCCCAACTGA CGTATCACTG 
CTCTCAGCGA GACCATCTGC TTTTCCTCCA GCTACTGTAA TACCAGAGGA AACAAGTACC 
AATGCTTCCA TCCCCGAAGT GTCCGGAGAG TTCCCTGAGT CTGGAGAGCA CCCAACCAGT 
GGCGAACCTT CAGCATCTGG AGCCCCAGAC ACGAGTGGAG AACCAACTTC CGTAGGTTTT 
GAACTGAGTG GAGAGCAATC CGGGATTGGA GAAAGTGGAT TACCATCTGT AGACCTGCAG 
AGCAGTGGCT TTGTACCTGG AGAAAGTGGC CTTCCCTCAG GGGATGTGAG CGGGTTGCCT 
TCTGGCATTG TTGATATCAG CGGCTTGCCT TCTGCAGAGG AAGAGGTAAC GGTGTCTGTT 
TCGAGGATAC CAGAAGTTAG TGGAATGCCA TCTGGAGCTG AAAGCAGTGG TCTGCATTCT 
GGATTTAGTG GAGAAATCTC TGGCACTGAG CTTATCAGTG GCCTGCCATC TGGAGAGGAA 
AGTGGGCTCG CCTCTGGTTT TCCCACCATC TCCCTCGTGG ATTCCACTTT GGTGGAGGTT 
GTAACGGCAG CACCGGGACG GCAAGAGGAG GGAAAAGGAT CAATTGGAGT CAGTGGTGAA 
GAAGAGCTGT CAGGGTTTCC GTCTGCAGAG TGGGACAGCA GTGGGGCCAG AGGGCTGCCC 
TCGGGAGCTG AAACCAGTGG GGAGCAATCC GGGGTGCCCG AGCTCAGTGG GGAGCATTCC 
GGGGTGCCTG GGCTCAGTGG AGAGCCTTTT GAGGTGCCTG AGCTCAGCGG GGAGCATTCC 
GGGGTGACTG AGCTCAGTGG GGAGCATTCT GGGCTTCCTG AGCTCAGTGG AGAGCCTTTT 
GGGGTGCCTG AGCTCAGCGG GTTTCCCTCC GGACTGGACA TCAGTGGGGA ACCATCTGGA 
GCACCCGAGG TGAGCGGCCC GGTGGACGTG AGCGGCCTTA CCTCTGGTGT TGATGGAAGT 
GGTGAGGTCT CAGGCGTTAC CTTTATAAGC ACCAGCCTGC AAGAAGTGAC AACCCAGTCA 
GTTGCAGAAG CAGAAGCAAA AGAAATTCTA GAGATCAGTG GACTGCCTTC AGGAGAGACA 
TCAGGCATGG TGTCTGGGAG CTTAGATGTC AGCGGTCAGC CTTCGGGACA CATAGACTTT 
GGTGGGAGCG CTTCTGGAGT GCTGGAGATG AGCGGATTTC CAAGCGGAGC GGTTGAGAGC 
AGTGGAGAAG CCTCTGGGGT TGAAGTCACC AGTGTCCTCG CGTCTGGAGA GGAAAGTGGG 
CTCACCTCAG GCTTTCCCAC CGTGTCTCTC GTGGATACCA CGTTGGTAGA AGTTGTAACG 
CAGACATCAG TTGCTCAAGA GGTGGGAGAA GGACCATCTG GGATGATAGA AATCAGTGGA 
TTTCTTTCTG GAGACAGAGG AGTATCTGGA GAAGGGTCTG GAGCTGTGCA GTCTAGTGGG 
CTTCCTTCAG GAACAGGAGA CTTCAGCGGA GAGCCATCCG GGATCCCGTA TTTCAGCGGA 
GACATTTCTG GAGCCACAGA TCTAAGCGGA CAACCTTCCG CAGTGACTGA TATTAGTGGG 
GAGGACTCGG GACTTCCAGA AGTCACTTTA GTCACATCCG ATTTAGTAGA AGTTGTGACA 
AGGCCAACAG TTTCACAGGA GCTGGGTGGG GAAACAGCTG TGACGTTTCC CTATGTCTTT 
GGGCCAAGTG GTGAGGGCTC TGCATCTGGA GACCTGAGTG GGGGAGCATC TGCAGAAGGT 
GGTATAGAAA CATCAGCAGC TTATGAAATC AGTGGTGAGA GCTCTGCCTT CCCTGAAACG 
AGTATAGAAA CATCCACAGA TCAAGAAATC AGTGGGGAAG CATCTGCATA CCCTGAGATT 
AGCGTAGAAA CATCCACGCA TCTGGAAACC AGTGGGGAAA CATCTGCATA CCCTGAGATT 
AGCACAGAGA CGTCCACCAT TCAGGAAGTT AGTGGGGAAA CGTCTGCCTT TCCTGAAATT 
AGCACAGAAA CATCCACAAT ACAAGAGATC AGTGGGGAAA CATCCGCATT TCCTGAAATT 
AGAATAGAAA CATCCACCTT TCAAGAAATC AGTGGGGAAA CGTCCGCATT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGCGGCGAAA CGTCTGCGTT TCCCGAGATT 
ACCATAGAAG CCTCCACAGT CCATGAAACC AGTGGAGAAA CATCTGCCTT TCCCGAAATT 
AGCATAGAAA CATCCACAGT CCATGAAATC AGTGGGGAAA GTTCTGCCTT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGGGGTGAAA CATCTGCGTT CCCTGAGATT 
ACCATAGAAG CCTCCACCAT TCAAGAGATC AGTGGAGAAA CATCTGCATT TCCTGAAATT 
AGCATAGCAA CTTCCACCGT CCGTGAAATC AGCGGCGAAA CGTCTGCCTT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGGGGTGAAA CATCTGCATT GCCTGAGATT 
ACCGTAGAGA CATCCACTGT CCATGAAACC AGTGGGGAAG CGTCTGCCTT TCCTGAAATT 
AGCATAGAAA CTTCCACAAG ACAAGAAGCC AGGGGTGAAG CATCCGCCTA CCCTGAGGTT 
AGCATAGAGG CATCCACAAC TCAAGAAGTA AGTGGGGAAA GTTCTGCCTT CCCTGAAATT 
AGCGTAGAGA CATCCACAAG TCAGGAAGCC CGCGGTGAAA CATCTGCCTT TCCTGAGATT 
GGCATAGAGA CATCCACAGC CCACGAAGGC AGTGGGGAAA CTCCTGGGCT GCCTGCTGTT 
AGCACTGACA CTGCTGCCAC GTCTCTGGCC AGTGGTGAGC CCTCCGGTGC TCCTGAGAAG 
GAAACTCCCG ACACAACATC ACATCTGATC ACGGGCGTTT CAGGGGAAAC CTCTGTCCCA 
GATGCTGTAA TCAGTACCAG TGCTCCAGAT GTTGAACTAG CGCAGGGACC CAGAAACACT 
GAAGAGACTC AGCTTGAAAT AGAGCCCTCC ACTCCTGCGG CATCTGGACA AGAGACAGAA 
ACAGCTGCTG TCCTCGACAA TCCCCATCTG CCAGCCACTG CTACTGCTGC CCTGCATCCA 
GCCTCCCAAG AAGCAGTAGA TGCACTGGGA CCCACGACAG AAGACACTGA TGAGTGCCAC 
TCAAGCCCCT GTCTGAATGG AGCTACCTGC GTTGATGGCA TCGACTCTTT CAAATGCTTA 
TGCCTTCCCA GCTACGGAGG GGACCTGTGT GAGATCGACC TGGCAAACTG TGAGGAAGGC 
TGGATCAAGT TCCAGGGCCA CTGCTACAGG CACTTTGAAG AGAGGGAGAC GTGGATGGAC 
GCAGAGTCCA GGTGCAGAGA ACATCAAGCC CACCTGAGCA GCATCATTAC TCCAGAGGAA 
CAAGAATTTG TGAACAGCCA TGCACAAGAC TATCAGTGGA TCGGCCTCAG TGACAGAGCC 
GTGGAGAATG ATTTCCGCTG GTCTGATGGA CACTCGCTGC AATTTGAGAA CTGGCGGCCC 
AACCAACCTG ACAACTTCTT CTCCGCGGGC GAGGACTGCG TTGTGATGAT CTGGCATGAG 
CAAGGCGAAT GGAACGACGT CCCCTGCAAC TATCACCTGC CTTTCACGTG CAAGAAGGGA 
ACAGTTGCCT GTGGGGACCC ACCTGTAGTG GAGAACGCGC GGACCTTTGG CCGGAAGAAG 
GACCGCTATG AGATCAACTC CCTGGTGCGG TACCAGTGTG ACCACGGCTA CATCCAGCGC 
CACGTGCCCA CCATCCGCTG CCAACCCAAC GGGCACTGGG AGGAACCGCG GATATCCTGC 
ACAAACCCCT CCAGCTACCA ACGCCGGCTA TACAAGAGGA GCCCCCGGAG CCGGTTGAGA 
CCCGGTGTCG TGCACAGACC CACCCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACAN ( XM_015291977.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015147463.1 Gallus gallus aggrecan (ACAN), transcript variant X1, mRNA.

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CloneID	OGa28230
Clone ID Related Accession (Same CDS sequence)	XM_015291977.1 , XM_015291977.2 , XM_015291978.2 , NM_204955.2 , XM_015291978.1
Accession Version	XM_015291977.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	aggrecan core protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455924.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACTC TACTACTAGT GTTTGTGTGT TTGCAAGCCA TCACCACAGC TGCCTCCGCA 
GAGCTCTCAG ACAGTAGCGA TGGCCTGGAA GTGAAGATAC CTGAGCAGTC TCCCCTGCGT 
GTTGTCCTGG GAAGCTCCCT GAACATCCCC TGCTATTTCA ACATCCCAGA GGAAGAGGAC 
ACCAATGCTC TGCTGACCCC CCGGATCAAA TGGAGCAAGC TTTCCAATGG AACAGAGATT 
GTCTTACTAG TGGCCACCGG TGGGAAGATC CGGCTCAATG CAGAGTACAG AGAGGTGATC 
TCCTTGCCCA ATTACCCTGC CATCCCCACT GATGCCACCT TGGAAATCAA GGCGCTGAGA 
TCCAACCACA CTGGGATTTA TCGCTGTGAA GTGATGTATG GGATTGAGGA CAGACAAGAC 
ACCATAGAGG TCCTGGTGAA AGGCGTCGTG TTCCACTACA GAGCAATCTC CACAAGGTAC 
ACTTTGAACT TCGAGAGGGC AAAGCAGGCC TGTATCCAGA ACAGTGCTGT CATTGCCACC 
CCTGAGCAGC TGCAGGCTGC CTACGAGGAT GGGTACGAGC AGTGCGATGC CGGCTGGCTG 
GCTGATCAGA CTGTCAGGTA CCCCATCCAT CTGCCCCGGG AGCGCTGCTA CGGTGACAAG 
GATGAGTTTC CAGGAGTGAG AACCTACGGT GTCCGTGAGA CAGATGAAAC CTATGATGTT 
TACTGCTATG CAGAGCAAAT GCAAGGCAAA GTCTTCTACG CCACCTCCCC CGAGAAGTTC 
ACCTTCCAGG AAGCTTTTGA CAAATGCCAC AGCTTGGGAG CCCGCCTGGC CACCACGGGC 
GAGCTGTACT TGGCCTGGAA GGATGGCATG GACATGTGCA GTGCGGGCTG GCTGGCTGAC 
CGCAGCGTTC GCTACCCCAT TTCCAGAGCA CGGCCCAATT GTGGAGGGAA CCTGGTGGGC 
GTGCGGACCG TTTACCTGAA CCCTGCCAAC CAGACAGGGT ACCCTCACCC CAGCTCACGC 
TACGATGCCA TCTGTTACAG TGGTGATGAC TTCGAGGCTC TGGTCCCAGG GCTGTTCACT 
GACGAGGTTG GGACTGAGCT GGGCAGTGCT TTTACCATAC AGACCGTCAC ACAGACTGAG 
GTGGAGCTGC CCCTGCCACG CAATGTCACA GAAGAGGAGG CCCGTGGCAG CATCGCTACC 
CTGGAGCCCA TGGAGATCAC AGCCACCGCC ACTGAGCTGT ATGAGGCCTT CACCGTCCTG 
CCTGACCTCT TTGCCACCAG TGTCACAGTA GAGACAGCTT CCCCAAGGGA AGAGAACGTG 
ACCAGAGAGG AGATCACAGG GATATGGGCT GTGCCTGAAG AGGTCACTAC ATCGGTCTCA 
GGCACTGCTT TCACCACCGG AATGGCAGAG GTGAGCTCAG TGGAAGAGGC CATAGCAGTG 
ACTGCCACAC CAGGACTGGA GTCTGCCTCT CCATTCACCA TAGAAGATCA TCTCGTGCAA 
GTGACAGCAG CCCCTGATGT TGCCCTCCTC CCCAGGCAGC CCATTTCCCC CACTGGTGTG 
GTGTTCCACT ACCGTGCTGC CACCAGCAGA TACGCCTTCT CCTTCATCCA AGCCCAGCAG 
GCCTGCCTGG AGAACAATGC CGTTATTGCC ACTCCCGAGC AGCTCCAGGC TGCCTACGAG 
GCTGGCTTTG ATCAGTGCGA TGCCGGCTGG CTGCGGGACC AGACGGTCAG GTATCCCATT 
GTGAATCCCC GCAGCAACTG TGTAGGAGAC AAAGAGAGCT CCCCAGGTGT GCGGTCATAC 
GGCATGCGCC CGGCCTCAGA GACCTACGAT GTGTACTGTT ACATCGACAG GCTAAAGGGT 
GAGGTGTTTT TTGCCACCCA GCCAGAGCAG TTCACCTTCC AAGAAGCCCA GCTGTACTGT 
GAAAGCCAAA ATGCCACGCT GGCCTCTGCT GGGCAACTCC ACGCTGCCTG GAAGCAGGGC 
CTGGATAGGT GCTACCCTGG GTGGCTGGCC GACGGCAGCT TGCGGTACCC CATTGTGAGC 
CCCCGACCTG CCTGCGGGGG GGATGCACCT GGTGTGAGGA CCATCTACCA GCACCACAAC 
CAGACGGGCT TTCCTGACCC TCTGTCACGG CATCACGCTT TCTGCTTCAG AGCTCTGCCA 
TCCGTAGTGG AGGAGGGGGT GACCTCACTC TTTGAAGAAG AGGTGATGGT AACCCAACTG 
ATCCCTGGAG TGGAAGGAAT ACCTTCTGGG GAGGAAACAA CTGTGGAGAC AGAGCTTTCC 
TCTGAGCCTG AGAATCAGAC AGCCCAGGGA ACGGAGGTCT TCCCAACTGA CGTATCACTG 
CTCTCAGCGA GACCATCTGC TTTTCCTCCA GCTACTGTAA TACCAGAGGA AACAAGTACC 
AATGCTTCCA TCCCCGAAGT GTCCGGAGAG TTCCCTGAGT CTGGAGAGCA CCCAACCAGT 
GGCGAACCTT CAGCATCTGG AGCCCCAGAC ACGAGTGGAG AACCAACTTC CGTAGGTTTT 
GAACTGAGTG GAGAGCAATC CGGGATTGGA GAAAGTGGAT TACCATCTGT AGACCTGCAG 
AGCAGTGGCT TTGTACCTGG AGAAAGTGGC CTTCCCTCAG GGGATGTGAG CGGGTTGCCT 
TCTGGCATTG TTGATATCAG CGGCTTGCCT TCTGCAGAGG AAGAGGTAAC GGTGTCTGTT 
TCGAGGATAC CAGAAGTTAG TGGAATGCCA TCTGGAGCTG AAAGCAGTGG TCTGCATTCT 
GGATTTAGTG GAGAAATCTC TGGCACTGAG CTTATCAGTG GCCTGCCATC TGGAGAGGAA 
AGTGGGCTCG CCTCTGGTTT TCCCACCATC TCCCTCGTGG ATTCCACTTT GGTGGAGGTT 
GTAACGGCAG CACCGGGACG GCAAGAGGAG GGAAAAGGAT CAATTGGAGT CAGTGGTGAA 
GAAGAGCTGT CAGGGTTTCC GTCTGCAGAG TGGGACAGCA GTGGGGCCAG AGGGCTGCCC 
TCGGGAGCTG AAACCAGTGG GGAGCAATCC GGGGTGCCCG AGCTCAGTGG GGAGCATTCC 
GGGGTGCCTG GGCTCAGTGG AGAGCCTTTT GAGGTGCCTG AGCTCAGCGG GGAGCATTCC 
GGGGTGACTG AGCTCAGTGG GGAGCATTCT GGGCTTCCTG AGCTCAGTGG AGAGCCTTTT 
GGGGTGCCTG AGCTCAGCGG GTTTCCCTCC GGACTGGACA TCAGTGGGGA ACCATCTGGA 
GCACCCGAGG TGAGCGGCCC GGTGGACGTG AGCGGCCTTA CCTCTGGTGT TGATGGAAGT 
GGTGAGGTCT CAGGCGTTAC CTTTATAAGC ACCAGCCTGC AAGAAGTGAC AACCCAGTCA 
GTTGCAGAAG CAGAAGCAAA AGAAATTCTA GAGATCAGTG GACTGCCTTC AGGAGAGACA 
TCAGGCATGG TGTCTGGGAG CTTAGATGTC AGCGGTCAGC CTTCGGGACA CATAGACTTT 
GGTGGGAGCG CTTCTGGAGT GCTGGAGATG AGCGGATTTC CAAGCGGAGC GGTTGAGAGC 
AGTGGAGAAG CCTCTGGGGT TGAAGTCACC AGTGTCCTCG CGTCTGGAGA GGAAAGTGGG 
CTCACCTCAG GCTTTCCCAC CGTGTCTCTC GTGGATACCA CGTTGGTAGA AGTTGTAACG 
CAGACATCAG TTGCTCAAGA GGTGGGAGAA GGACCATCTG GGATGATAGA AATCAGTGGA 
TTTCTTTCTG GAGACAGAGG AGTATCTGGA GAAGGGTCTG GAGCTGTGCA GTCTAGTGGG 
CTTCCTTCAG GAACAGGAGA CTTCAGCGGA GAGCCATCCG GGATCCCGTA TTTCAGCGGA 
GACATTTCTG GAGCCACAGA TCTAAGCGGA CAACCTTCCG CAGTGACTGA TATTAGTGGG 
GAGGACTCGG GACTTCCAGA AGTCACTTTA GTCACATCCG ATTTAGTAGA AGTTGTGACA 
AGGCCAACAG TTTCACAGGA GCTGGGTGGG GAAACAGCTG TGACGTTTCC CTATGTCTTT 
GGGCCAAGTG GTGAGGGCTC TGCATCTGGA GACCTGAGTG GGGGAGCATC TGCAGAAGGT 
GGTATAGAAA CATCAGCAGC TTATGAAATC AGTGGTGAGA GCTCTGCCTT CCCTGAAACG 
AGTATAGAAA CATCCACAGA TCAAGAAATC AGTGGGGAAG CATCTGCATA CCCTGAGATT 
AGCGTAGAAA CATCCACGCA TCTGGAAACC AGTGGGGAAA CATCTGCATA CCCTGAGATT 
AGCACAGAGA CGTCCACCAT TCAGGAAGTT AGTGGGGAAA CGTCTGCCTT TCCTGAAATT 
AGCACAGAAA CATCCACAAT ACAAGAGATC AGTGGGGAAA CATCCGCATT TCCTGAAATT 
AGAATAGAAA CATCCACCTT TCAAGAAATC AGTGGGGAAA CGTCCGCATT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGCGGCGAAA CGTCTGCGTT TCCCGAGATT 
ACCATAGAAG CCTCCACAGT CCATGAAACC AGTGGAGAAA CATCTGCCTT TCCCGAAATT 
AGCATAGAAA CATCCACAGT CCATGAAATC AGTGGGGAAA GTTCTGCCTT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGGGGTGAAA CATCTGCGTT CCCTGAGATT 
ACCATAGAAG CCTCCACCAT TCAAGAGATC AGTGGAGAAA CATCTGCATT TCCTGAAATT 
AGCATAGCAA CTTCCACCGT CCGTGAAATC AGCGGCGAAA CGTCTGCCTT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGGGGTGAAA CATCTGCATT GCCTGAGATT 
ACCGTAGAGA CATCCACTGT CCATGAAACC AGTGGGGAAG CGTCTGCCTT TCCTGAAATT 
AGCATAGAAA CTTCCACAAG ACAAGAAGCC AGGGGTGAAG CATCCGCCTA CCCTGAGGTT 
AGCATAGAGG CATCCACAAC TCAAGAAGTA AGTGGGGAAA GTTCTGCCTT CCCTGAAATT 
AGCGTAGAGA CATCCACAAG TCAGGAAGCC CGCGGTGAAA CATCTGCCTT TCCTGAGATT 
GGCATAGAGA CATCCACAGC CCACGAAGGC AGTGGGGAAA CTCCTGGGCT GCCTGCTGTT 
AGCACTGACA CTGCTGCCAC GTCTCTGGCC AGTGGTGAGC CCTCCGGTGC TCCTGAGAAG 
GAAACTCCCG ACACAACATC ACATCTGATC ACGGGCGTTT CAGGGGAAAC CTCTGTCCCA 
GATGCTGTAA TCAGTACCAG TGCTCCAGAT GTTGAACTAG CGCAGGGACC CAGAAACACT 
GAAGAGACTC AGCTTGAAAT AGAGCCCTCC ACTCCTGCGG CATCTGGACA AGAGACAGAA 
ACAGCTGCTG TCCTCGACAA TCCCCATCTG CCAGCCACTG CTACTGCTGC CCTGCATCCA 
GCCTCCCAAG AAGCAGTAGA TGCACTGGGA CCCACGACAG AAGACACTGA TGAGTGCCAC 
TCAAGCCCCT GTCTGAATGG AGCTACCTGC GTTGATGGCA TCGACTCTTT CAAATGCTTA 
TGCCTTCCCA GCTACGGAGG GGACCTGTGT GAGATCGACC TGGCAAACTG TGAGGAAGGC 
TGGATCAAGT TCCAGGGCCA CTGCTACAGG CACTTTGAAG AGAGGGAGAC GTGGATGGAC 
GCAGAGTCCA GGTGCAGAGA ACATCAAGCC CACCTGAGCA GCATCATTAC TCCAGAGGAA 
CAAGAATTTG TGAACAGCCA TGCACAAGAC TATCAGTGGA TCGGCCTCAG TGACAGAGCC 
GTGGAGAATG ATTTCCGCTG GTCTGATGGA CACTCGCTGC AATTTGAGAA CTGGCGGCCC 
AACCAACCTG ACAACTTCTT CTCCGCGGGC GAGGACTGCG TTGTGATGAT CTGGCATGAG 
CAAGGCGAAT GGAACGACGT CCCCTGCAAC TATCACCTGC CTTTCACGTG CAAGAAGGGA 
ACAGTTGCCT GTGGGGACCC ACCTGTAGTG GAGAACGCGC GGACCTTTGG CCGGAAGAAG 
GACCGCTATG AGATCAACTC CCTGGTGCGG TACCAGTGTG ACCACGGCTA CATCCAGCGC 
CACGTGCCCA CCATCCGCTG CCAACCCAAC GGGCACTGGG AGGAACCGCG GATATCCTGC 
ACAAACCCCT CCAGCTACCA ACGCCGGCTA TACAAGAGGA GCCCCCGGAG CCGGTTGAGA 
CCCGGTGTCG TGCACAGACC CACCCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015147463.1
CDS	1383..7652
Translation	MTTLLLVFVCLQAITTAASAELSDSSDGLEVKIPEQSPLRVVLGSSLNIPCYFNIPEEEDTNALLTPRIKWSKLSNGTEIVLLVATGGKIRLNAEYREVISLPNYPAIPTDATLEIKALRSNHTGIYRCEVMYGIEDRQDTIEVLVKGVVFHYRAISTRYTLNFERAKQACIQNSAVIATPEQLQAAYEDGYEQCDAGWLADQTVRYPIHLPRERCYGDKDEFPGVRTYGVRETDETYDVYCYAEQMQGKVFYATSPEKFTFQEAFDKCHSLGARLATTGELYLAWKDGMDMCSAGWLADRSVRYPISRARPNCGGNLVGVRTVYLNPANQTGYPHPSSRYDAICYSGDDFEALVPGLFTDEVGTELGSAFTIQTVTQTEVELPLPRNVTEEEARGSIATLEPMEITATATELYEAFTVLPDLFATSVTVETASPREENVTREEITGIWAVPEEVTTSVSGTAFTTGMAEVSSVEEAIAVTATPGLESASPFTIEDHLVQVTAAPDVALLPRQPISPTGVVFHYRAATSRYAFSFIQAQQACLENNAVIATPEQLQAAYEAGFDQCDAGWLRDQTVRYPIVNPRSNCVGDKESSPGVRSYGMRPASETYDVYCYIDRLKGEVFFATQPEQFTFQEAQLYCESQNATLASAGQLHAAWKQGLDRCYPGWLADGSLRYPIVSPRPACGGDAPGVRTIYQHHNQTGFPDPLSRHHAFCFRALPSVVEEGVTSLFEEEVMVTQLIPGVEGIPSGEETTVETELSSEPENQTAQGTEVFPTDVSLLSARPSAFPPATVIPEETSTNASIPEVSGEFPESGEHPTSGEPSASGAPDTSGEPTSVGFELSGEQSGIGESGLPSVDLQSSGFVPGESGLPSGDVSGLPSGIVDISGLPSAEEEVTVSVSRIPEVSGMPSGAESSGLHSGFSGEISGTELISGLPSGEESGLASGFPTISLVDSTLVEVVTAAPGRQEEGKGSIGVSGEEELSGFPSAEWDSSGARGLPSGAETSGEQSGVPELSGEHSGVPGLSGEPFEVPELSGEHSGVTELSGEHSGLPELSGEPFGVPELSGFPSGLDISGEPSGAPEVSGPVDVSGLTSGVDGSGEVSGVTFISTSLQEVTTQSVAEAEAKEILEISGLPSGETSGMVSGSLDVSGQPSGHIDFGGSASGVLEMSGFPSGAVESSGEASGVEVTSVLASGEESGLTSGFPTVSLVDTTLVEVVTQTSVAQEVGEGPSGMIEISGFLSGDRGVSGEGSGAVQSSGLPSGTGDFSGEPSGIPYFSGDISGATDLSGQPSAVTDISGEDSGLPEVTLVTSDLVEVVTRPTVSQELGGETAVTFPYVFGPSGEGSASGDLSGGASAEGGIETSAAYEISGESSAFPETSIETSTDQEISGEASAYPEISVETSTHLETSGETSAYPEISTETSTIQEVSGETSAFPEISTETSTIQEISGETSAFPEIRIETSTFQEISGETSAFPEIRIETSTSQEARGETSAFPEITIEASTVHETSGETSAFPEISIETSTVHEISGESSAFPEIRIETSTSQEARGETSAFPEITIEASTIQEISGETSAFPEISIATSTVREISGETSAFPEIRIETSTSQEARGETSALPEITVETSTVHETSGEASAFPEISIETSTRQEARGEASAYPEVSIEASTTQEVSGESSAFPEISVETSTSQEARGETSAFPEIGIETSTAHEGSGETPGLPAVSTDTAATSLASGEPSGAPEKETPDTTSHLITGVSGETSVPDAVISTSAPDVELAQGPRNTEETQLEIEPSTPAASGQETETAAVLDNPHLPATATAALHPASQEAVDALGPTTEDTDECHSSPCLNGATCVDGIDSFKCLCLPSYGGDLCEIDLANCEEGWIKFQGHCYRHFEERETWMDAESRCREHQAHLSSIITPEEQEFVNSHAQDYQWIGLSDRAVENDFRWSDGHSLQFENWRPNQPDNFFSAGEDCVVMIWHEQGEWNDVPCNYHLPFTCKKGTVACGDPPVVENARTFGRKKDRYEINSLVRYQCDHGYIQRHVPTIRCQPNGHWEEPRISCTNPSSYQRRLYKRSPRSRLRPGVVHRPTH

Target ORF information:

RefSeq Version	XM_015291977.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus aggrecan (ACAN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291977.1

ORF Insert Sequence:

ATGACCACTC TACTACTAGT GTTTGTGTGT TTGCAAGCCA TCACCACAGC TGCCTCCGCA 
GAGCTCTCAG ACAGTAGCGA TGGCCTGGAA GTGAAGATAC CTGAGCAGTC TCCCCTGCGT 
GTTGTCCTGG GAAGCTCCCT GAACATCCCC TGCTATTTCA ACATCCCAGA GGAAGAGGAC 
ACCAATGCTC TGCTGACCCC CCGGATCAAA TGGAGCAAGC TTTCCAATGG AACAGAGATT 
GTCTTACTAG TGGCCACCGG TGGGAAGATC CGGCTCAATG CAGAGTACAG AGAGGTGATC 
TCCTTGCCCA ATTACCCTGC CATCCCCACT GATGCCACCT TGGAAATCAA GGCGCTGAGA 
TCCAACCACA CTGGGATTTA TCGCTGTGAA GTGATGTATG GGATTGAGGA CAGACAAGAC 
ACCATAGAGG TCCTGGTGAA AGGCGTCGTG TTCCACTACA GAGCAATCTC CACAAGGTAC 
ACTTTGAACT TCGAGAGGGC AAAGCAGGCC TGTATCCAGA ACAGTGCTGT CATTGCCACC 
CCTGAGCAGC TGCAGGCTGC CTACGAGGAT GGGTACGAGC AGTGCGATGC CGGCTGGCTG 
GCTGATCAGA CTGTCAGGTA CCCCATCCAT CTGCCCCGGG AGCGCTGCTA CGGTGACAAG 
GATGAGTTTC CAGGAGTGAG AACCTACGGT GTCCGTGAGA CAGATGAAAC CTATGATGTT 
TACTGCTATG CAGAGCAAAT GCAAGGCAAA GTCTTCTACG CCACCTCCCC CGAGAAGTTC 
ACCTTCCAGG AAGCTTTTGA CAAATGCCAC AGCTTGGGAG CCCGCCTGGC CACCACGGGC 
GAGCTGTACT TGGCCTGGAA GGATGGCATG GACATGTGCA GTGCGGGCTG GCTGGCTGAC 
CGCAGCGTTC GCTACCCCAT TTCCAGAGCA CGGCCCAATT GTGGAGGGAA CCTGGTGGGC 
GTGCGGACCG TTTACCTGAA CCCTGCCAAC CAGACAGGGT ACCCTCACCC CAGCTCACGC 
TACGATGCCA TCTGTTACAG TGGTGATGAC TTCGAGGCTC TGGTCCCAGG GCTGTTCACT 
GACGAGGTTG GGACTGAGCT GGGCAGTGCT TTTACCATAC AGACCGTCAC ACAGACTGAG 
GTGGAGCTGC CCCTGCCACG CAATGTCACA GAAGAGGAGG CCCGTGGCAG CATCGCTACC 
CTGGAGCCCA TGGAGATCAC AGCCACCGCC ACTGAGCTGT ATGAGGCCTT CACCGTCCTG 
CCTGACCTCT TTGCCACCAG TGTCACAGTA GAGACAGCTT CCCCAAGGGA AGAGAACGTG 
ACCAGAGAGG AGATCACAGG GATATGGGCT GTGCCTGAAG AGGTCACTAC ATCGGTCTCA 
GGCACTGCTT TCACCACCGG AATGGCAGAG GTGAGCTCAG TGGAAGAGGC CATAGCAGTG 
ACTGCCACAC CAGGACTGGA GTCTGCCTCT CCATTCACCA TAGAAGATCA TCTCGTGCAA 
GTGACAGCAG CCCCTGATGT TGCCCTCCTC CCCAGGCAGC CCATTTCCCC CACTGGTGTG 
GTGTTCCACT ACCGTGCTGC CACCAGCAGA TACGCCTTCT CCTTCATCCA AGCCCAGCAG 
GCCTGCCTGG AGAACAATGC CGTTATTGCC ACTCCCGAGC AGCTCCAGGC TGCCTACGAG 
GCTGGCTTTG ATCAGTGCGA TGCCGGCTGG CTGCGGGACC AGACGGTCAG GTATCCCATT 
GTGAATCCCC GCAGCAACTG TGTAGGAGAC AAAGAGAGCT CCCCAGGTGT GCGGTCATAC 
GGCATGCGCC CGGCCTCAGA GACCTACGAT GTGTACTGTT ACATCGACAG GCTAAAGGGT 
GAGGTGTTTT TTGCCACCCA GCCAGAGCAG TTCACCTTCC AAGAAGCCCA GCTGTACTGT 
GAAAGCCAAA ATGCCACGCT GGCCTCTGCT GGGCAACTCC ACGCTGCCTG GAAGCAGGGC 
CTGGATAGGT GCTACCCTGG GTGGCTGGCC GACGGCAGCT TGCGGTACCC CATTGTGAGC 
CCCCGACCTG CCTGCGGGGG GGATGCACCT GGTGTGAGGA CCATCTACCA GCACCACAAC 
CAGACGGGCT TTCCTGACCC TCTGTCACGG CATCACGCTT TCTGCTTCAG AGCTCTGCCA 
TCCGTAGTGG AGGAGGGGGT GACCTCACTC TTTGAAGAAG AGGTGATGGT AACCCAACTG 
ATCCCTGGAG TGGAAGGAAT ACCTTCTGGG GAGGAAACAA CTGTGGAGAC AGAGCTTTCC 
TCTGAGCCTG AGAATCAGAC AGCCCAGGGA ACGGAGGTCT TCCCAACTGA CGTATCACTG 
CTCTCAGCGA GACCATCTGC TTTTCCTCCA GCTACTGTAA TACCAGAGGA AACAAGTACC 
AATGCTTCCA TCCCCGAAGT GTCCGGAGAG TTCCCTGAGT CTGGAGAGCA CCCAACCAGT 
GGCGAACCTT CAGCATCTGG AGCCCCAGAC ACGAGTGGAG AACCAACTTC CGTAGGTTTT 
GAACTGAGTG GAGAGCAATC CGGGATTGGA GAAAGTGGAT TACCATCTGT AGACCTGCAG 
AGCAGTGGCT TTGTACCTGG AGAAAGTGGC CTTCCCTCAG GGGATGTGAG CGGGTTGCCT 
TCTGGCATTG TTGATATCAG CGGCTTGCCT TCTGCAGAGG AAGAGGTAAC GGTGTCTGTT 
TCGAGGATAC CAGAAGTTAG TGGAATGCCA TCTGGAGCTG AAAGCAGTGG TCTGCATTCT 
GGATTTAGTG GAGAAATCTC TGGCACTGAG CTTATCAGTG GCCTGCCATC TGGAGAGGAA 
AGTGGGCTCG CCTCTGGTTT TCCCACCATC TCCCTCGTGG ATTCCACTTT GGTGGAGGTT 
GTAACGGCAG CACCGGGACG GCAAGAGGAG GGAAAAGGAT CAATTGGAGT CAGTGGTGAA 
GAAGAGCTGT CAGGGTTTCC GTCTGCAGAG TGGGACAGCA GTGGGGCCAG AGGGCTGCCC 
TCGGGAGCTG AAACCAGTGG GGAGCAATCC GGGGTGCCCG AGCTCAGTGG GGAGCATTCC 
GGGGTGCCTG GGCTCAGTGG AGAGCCTTTT GAGGTGCCTG AGCTCAGCGG GGAGCATTCC 
GGGGTGACTG AGCTCAGTGG GGAGCATTCT GGGCTTCCTG AGCTCAGTGG AGAGCCTTTT 
GGGGTGCCTG AGCTCAGCGG GTTTCCCTCC GGACTGGACA TCAGTGGGGA ACCATCTGGA 
GCACCCGAGG TGAGCGGCCC GGTGGACGTG AGCGGCCTTA CCTCTGGTGT TGATGGAAGT 
GGTGAGGTCT CAGGCGTTAC CTTTATAAGC ACCAGCCTGC AAGAAGTGAC AACCCAGTCA 
GTTGCAGAAG CAGAAGCAAA AGAAATTCTA GAGATCAGTG GACTGCCTTC AGGAGAGACA 
TCAGGCATGG TGTCTGGGAG CTTAGATGTC AGCGGTCAGC CTTCGGGACA CATAGACTTT 
GGTGGGAGCG CTTCTGGAGT GCTGGAGATG AGCGGATTTC CAAGCGGAGC GGTTGAGAGC 
AGTGGAGAAG CCTCTGGGGT TGAAGTCACC AGTGTCCTCG CGTCTGGAGA GGAAAGTGGG 
CTCACCTCAG GCTTTCCCAC CGTGTCTCTC GTGGATACCA CGTTGGTAGA AGTTGTAACG 
CAGACATCAG TTGCTCAAGA GGTGGGAGAA GGACCATCTG GGATGATAGA AATCAGTGGA 
TTTCTTTCTG GAGACAGAGG AGTATCTGGA GAAGGGTCTG GAGCTGTGCA GTCTAGTGGG 
CTTCCTTCAG GAACAGGAGA CTTCAGCGGA GAGCCATCCG GGATCCCGTA TTTCAGCGGA 
GACATTTCTG GAGCCACAGA TCTAAGCGGA CAACCTTCCG CAGTGACTGA TATTAGTGGG 
GAGGACTCGG GACTTCCAGA AGTCACTTTA GTCACATCCG ATTTAGTAGA AGTTGTGACA 
AGGCCAACAG TTTCACAGGA GCTGGGTGGG GAAACAGCTG TGACGTTTCC CTATGTCTTT 
GGGCCAAGTG GTGAGGGCTC TGCATCTGGA GACCTGAGTG GGGGAGCATC TGCAGAAGGT 
GGTATAGAAA CATCAGCAGC TTATGAAATC AGTGGTGAGA GCTCTGCCTT CCCTGAAACG 
AGTATAGAAA CATCCACAGA TCAAGAAATC AGTGGGGAAG CATCTGCATA CCCTGAGATT 
AGCGTAGAAA CATCCACGCA TCTGGAAACC AGTGGGGAAA CATCTGCATA CCCTGAGATT 
AGCACAGAGA CGTCCACCAT TCAGGAAGTT AGTGGGGAAA CGTCTGCCTT TCCTGAAATT 
AGCACAGAAA CATCCACAAT ACAAGAGATC AGTGGGGAAA CATCCGCATT TCCTGAAATT 
AGAATAGAAA CATCCACCTT TCAAGAAATC AGTGGGGAAA CGTCCGCATT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGCGGCGAAA CGTCTGCGTT TCCCGAGATT 
ACCATAGAAG CCTCCACAGT CCATGAAACC AGTGGAGAAA CATCTGCCTT TCCCGAAATT 
AGCATAGAAA CATCCACAGT CCATGAAATC AGTGGGGAAA GTTCTGCCTT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGGGGTGAAA CATCTGCGTT CCCTGAGATT 
ACCATAGAAG CCTCCACCAT TCAAGAGATC AGTGGAGAAA CATCTGCATT TCCTGAAATT 
AGCATAGCAA CTTCCACCGT CCGTGAAATC AGCGGCGAAA CGTCTGCCTT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGGGGTGAAA CATCTGCATT GCCTGAGATT 
ACCGTAGAGA CATCCACTGT CCATGAAACC AGTGGGGAAG CGTCTGCCTT TCCTGAAATT 
AGCATAGAAA CTTCCACAAG ACAAGAAGCC AGGGGTGAAG CATCCGCCTA CCCTGAGGTT 
AGCATAGAGG CATCCACAAC TCAAGAAGTA AGTGGGGAAA GTTCTGCCTT CCCTGAAATT 
AGCGTAGAGA CATCCACAAG TCAGGAAGCC CGCGGTGAAA CATCTGCCTT TCCTGAGATT 
GGCATAGAGA CATCCACAGC CCACGAAGGC AGTGGGGAAA CTCCTGGGCT GCCTGCTGTT 
AGCACTGACA CTGCTGCCAC GTCTCTGGCC AGTGGTGAGC CCTCCGGTGC TCCTGAGAAG 
GAAACTCCCG ACACAACATC ACATCTGATC ACGGGCGTTT CAGGGGAAAC CTCTGTCCCA 
GATGCTGTAA TCAGTACCAG TGCTCCAGAT GTTGAACTAG CGCAGGGACC CAGAAACACT 
GAAGAGACTC AGCTTGAAAT AGAGCCCTCC ACTCCTGCGG CATCTGGACA AGAGACAGAA 
ACAGCTGCTG TCCTCGACAA TCCCCATCTG CCAGCCACTG CTACTGCTGC CCTGCATCCA 
GCCTCCCAAG AAGCAGTAGA TGCACTGGGA CCCACGACAG AAGACACTGA TGAGTGCCAC 
TCAAGCCCCT GTCTGAATGG AGCTACCTGC GTTGATGGCA TCGACTCTTT CAAATGCTTA 
TGCCTTCCCA GCTACGGAGG GGACCTGTGT GAGATCGACC TGGCAAACTG TGAGGAAGGC 
TGGATCAAGT TCCAGGGCCA CTGCTACAGG CACTTTGAAG AGAGGGAGAC GTGGATGGAC 
GCAGAGTCCA GGTGCAGAGA ACATCAAGCC CACCTGAGCA GCATCATTAC TCCAGAGGAA 
CAAGAATTTG TGAACAGCCA TGCACAAGAC TATCAGTGGA TCGGCCTCAG TGACAGAGCC 
GTGGAGAATG ATTTCCGCTG GTCTGATGGA CACTCGCTGC AATTTGAGAA CTGGCGGCCC 
AACCAACCTG ACAACTTCTT CTCCGCGGGC GAGGACTGCG TTGTGATGAT CTGGCATGAG 
CAAGGCGAAT GGAACGACGT CCCCTGCAAC TATCACCTGC CTTTCACGTG CAAGAAGGGA 
ACAGTTGCCT GTGGGGACCC ACCTGTAGTG GAGAACGCGC GGACCTTTGG CCGGAAGAAG 
GACCGCTATG AGATCAACTC CCTGGTGCGG TACCAGTGTG ACCACGGCTA CATCCAGCGC 
CACGTGCCCA CCATCCGCTG CCAACCCAAC GGGCACTGGG AGGAACCGCG GATATCCTGC 
ACAAACCCCT CCAGCTACCA ACGCCGGCTA TACAAGAGGA GCCCCCGGAG CCGGTTGAGA 
CCCGGTGTCG TGCACAGACC CACCCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACAN ( XM_015291977.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015147463.1 Gallus gallus aggrecan (ACAN), transcript variant X1, mRNA.

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CloneID	OGa28230
Clone ID Related Accession (Same CDS sequence)	XM_015291977.1 , XM_015291977.2 , XM_015291978.2 , NM_204955.2 , XM_015291978.1
Accession Version	XM_015291977.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	aggrecan core protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006097.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291977.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACTC TACTACTAGT GTTTGTGTGT TTGCAAGCCA TCACCACAGC TGCCTCCGCA 
GAGCTCTCAG ACAGTAGCGA TGGCCTGGAA GTGAAGATAC CTGAGCAGTC TCCCCTGCGT 
GTTGTCCTGG GAAGCTCCCT GAACATCCCC TGCTATTTCA ACATCCCAGA GGAAGAGGAC 
ACCAATGCTC TGCTGACCCC CCGGATCAAA TGGAGCAAGC TTTCCAATGG AACAGAGATT 
GTCTTACTAG TGGCCACCGG TGGGAAGATC CGGCTCAATG CAGAGTACAG AGAGGTGATC 
TCCTTGCCCA ATTACCCTGC CATCCCCACT GATGCCACCT TGGAAATCAA GGCGCTGAGA 
TCCAACCACA CTGGGATTTA TCGCTGTGAA GTGATGTATG GGATTGAGGA CAGACAAGAC 
ACCATAGAGG TCCTGGTGAA AGGCGTCGTG TTCCACTACA GAGCAATCTC CACAAGGTAC 
ACTTTGAACT TCGAGAGGGC AAAGCAGGCC TGTATCCAGA ACAGTGCTGT CATTGCCACC 
CCTGAGCAGC TGCAGGCTGC CTACGAGGAT GGGTACGAGC AGTGCGATGC CGGCTGGCTG 
GCTGATCAGA CTGTCAGGTA CCCCATCCAT CTGCCCCGGG AGCGCTGCTA CGGTGACAAG 
GATGAGTTTC CAGGAGTGAG AACCTACGGT GTCCGTGAGA CAGATGAAAC CTATGATGTT 
TACTGCTATG CAGAGCAAAT GCAAGGCAAA GTCTTCTACG CCACCTCCCC CGAGAAGTTC 
ACCTTCCAGG AAGCTTTTGA CAAATGCCAC AGCTTGGGAG CCCGCCTGGC CACCACGGGC 
GAGCTGTACT TGGCCTGGAA GGATGGCATG GACATGTGCA GTGCGGGCTG GCTGGCTGAC 
CGCAGCGTTC GCTACCCCAT TTCCAGAGCA CGGCCCAATT GTGGAGGGAA CCTGGTGGGC 
GTGCGGACCG TTTACCTGAA CCCTGCCAAC CAGACAGGGT ACCCTCACCC CAGCTCACGC 
TACGATGCCA TCTGTTACAG TGGTGATGAC TTCGAGGCTC TGGTCCCAGG GCTGTTCACT 
GACGAGGTTG GGACTGAGCT GGGCAGTGCT TTTACCATAC AGACCGTCAC ACAGACTGAG 
GTGGAGCTGC CCCTGCCACG CAATGTCACA GAAGAGGAGG CCCGTGGCAG CATCGCTACC 
CTGGAGCCCA TGGAGATCAC AGCCACCGCC ACTGAGCTGT ATGAGGCCTT CACCGTCCTG 
CCTGACCTCT TTGCCACCAG TGTCACAGTA GAGACAGCTT CCCCAAGGGA AGAGAACGTG 
ACCAGAGAGG AGATCACAGG GATATGGGCT GTGCCTGAAG AGGTCACTAC ATCGGTCTCA 
GGCACTGCTT TCACCACCGG AATGGCAGAG GTGAGCTCAG TGGAAGAGGC CATAGCAGTG 
ACTGCCACAC CAGGACTGGA GTCTGCCTCT CCATTCACCA TAGAAGATCA TCTCGTGCAA 
GTGACAGCAG CCCCTGATGT TGCCCTCCTC CCCAGGCAGC CCATTTCCCC CACTGGTGTG 
GTGTTCCACT ACCGTGCTGC CACCAGCAGA TACGCCTTCT CCTTCATCCA AGCCCAGCAG 
GCCTGCCTGG AGAACAATGC CGTTATTGCC ACTCCCGAGC AGCTCCAGGC TGCCTACGAG 
GCTGGCTTTG ATCAGTGCGA TGCCGGCTGG CTGCGGGACC AGACGGTCAG GTATCCCATT 
GTGAATCCCC GCAGCAACTG TGTAGGAGAC AAAGAGAGCT CCCCAGGTGT GCGGTCATAC 
GGCATGCGCC CGGCCTCAGA GACCTACGAT GTGTACTGTT ACATCGACAG GCTAAAGGGT 
GAGGTGTTTT TTGCCACCCA GCCAGAGCAG TTCACCTTCC AAGAAGCCCA GCTGTACTGT 
GAAAGCCAAA ATGCCACGCT GGCCTCTGCT GGGCAACTCC ACGCTGCCTG GAAGCAGGGC 
CTGGATAGGT GCTACCCTGG GTGGCTGGCC GACGGCAGCT TGCGGTACCC CATTGTGAGC 
CCCCGACCTG CCTGCGGGGG GGATGCACCT GGTGTGAGGA CCATCTACCA GCACCACAAC 
CAGACGGGCT TTCCTGACCC TCTGTCACGG CATCACGCTT TCTGCTTCAG AGCTCTGCCA 
TCCGTAGTGG AGGAGGGGGT GACCTCACTC TTTGAAGAAG AGGTGATGGT AACCCAACTG 
ATCCCTGGAG TGGAAGGAAT ACCTTCTGGG GAGGAAACAA CTGTGGAGAC AGAGCTTTCC 
TCTGAGCCTG AGAATCAGAC AGCCCAGGGA ACGGAGGTCT TCCCAACTGA CGTATCACTG 
CTCTCAGCGA GACCATCTGC TTTTCCTCCA GCTACTGTAA TACCAGAGGA AACAAGTACC 
AATGCTTCCA TCCCCGAAGT GTCCGGAGAG TTCCCTGAGT CTGGAGAGCA CCCAACCAGT 
GGCGAACCTT CAGCATCTGG AGCCCCAGAC ACGAGTGGAG AACCAACTTC CGTAGGTTTT 
GAACTGAGTG GAGAGCAATC CGGGATTGGA GAAAGTGGAT TACCATCTGT AGACCTGCAG 
AGCAGTGGCT TTGTACCTGG AGAAAGTGGC CTTCCCTCAG GGGATGTGAG CGGGTTGCCT 
TCTGGCATTG TTGATATCAG CGGCTTGCCT TCTGCAGAGG AAGAGGTAAC GGTGTCTGTT 
TCGAGGATAC CAGAAGTTAG TGGAATGCCA TCTGGAGCTG AAAGCAGTGG TCTGCATTCT 
GGATTTAGTG GAGAAATCTC TGGCACTGAG CTTATCAGTG GCCTGCCATC TGGAGAGGAA 
AGTGGGCTCG CCTCTGGTTT TCCCACCATC TCCCTCGTGG ATTCCACTTT GGTGGAGGTT 
GTAACGGCAG CACCGGGACG GCAAGAGGAG GGAAAAGGAT CAATTGGAGT CAGTGGTGAA 
GAAGAGCTGT CAGGGTTTCC GTCTGCAGAG TGGGACAGCA GTGGGGCCAG AGGGCTGCCC 
TCGGGAGCTG AAACCAGTGG GGAGCAATCC GGGGTGCCCG AGCTCAGTGG GGAGCATTCC 
GGGGTGCCTG GGCTCAGTGG AGAGCCTTTT GAGGTGCCTG AGCTCAGCGG GGAGCATTCC 
GGGGTGACTG AGCTCAGTGG GGAGCATTCT GGGCTTCCTG AGCTCAGTGG AGAGCCTTTT 
GGGGTGCCTG AGCTCAGCGG GTTTCCCTCC GGACTGGACA TCAGTGGGGA ACCATCTGGA 
GCACCCGAGG TGAGCGGCCC GGTGGACGTG AGCGGCCTTA CCTCTGGTGT TGATGGAAGT 
GGTGAGGTCT CAGGCGTTAC CTTTATAAGC ACCAGCCTGC AAGAAGTGAC AACCCAGTCA 
GTTGCAGAAG CAGAAGCAAA AGAAATTCTA GAGATCAGTG GACTGCCTTC AGGAGAGACA 
TCAGGCATGG TGTCTGGGAG CTTAGATGTC AGCGGTCAGC CTTCGGGACA CATAGACTTT 
GGTGGGAGCG CTTCTGGAGT GCTGGAGATG AGCGGATTTC CAAGCGGAGC GGTTGAGAGC 
AGTGGAGAAG CCTCTGGGGT TGAAGTCACC AGTGTCCTCG CGTCTGGAGA GGAAAGTGGG 
CTCACCTCAG GCTTTCCCAC CGTGTCTCTC GTGGATACCA CGTTGGTAGA AGTTGTAACG 
CAGACATCAG TTGCTCAAGA GGTGGGAGAA GGACCATCTG GGATGATAGA AATCAGTGGA 
TTTCTTTCTG GAGACAGAGG AGTATCTGGA GAAGGGTCTG GAGCTGTGCA GTCTAGTGGG 
CTTCCTTCAG GAACAGGAGA CTTCAGCGGA GAGCCATCCG GGATCCCGTA TTTCAGCGGA 
GACATTTCTG GAGCCACAGA TCTAAGCGGA CAACCTTCCG CAGTGACTGA TATTAGTGGG 
GAGGACTCGG GACTTCCAGA AGTCACTTTA GTCACATCCG ATTTAGTAGA AGTTGTGACA 
AGGCCAACAG TTTCACAGGA GCTGGGTGGG GAAACAGCTG TGACGTTTCC CTATGTCTTT 
GGGCCAAGTG GTGAGGGCTC TGCATCTGGA GACCTGAGTG GGGGAGCATC TGCAGAAGGT 
GGTATAGAAA CATCAGCAGC TTATGAAATC AGTGGTGAGA GCTCTGCCTT CCCTGAAACG 
AGTATAGAAA CATCCACAGA TCAAGAAATC AGTGGGGAAG CATCTGCATA CCCTGAGATT 
AGCGTAGAAA CATCCACGCA TCTGGAAACC AGTGGGGAAA CATCTGCATA CCCTGAGATT 
AGCACAGAGA CGTCCACCAT TCAGGAAGTT AGTGGGGAAA CGTCTGCCTT TCCTGAAATT 
AGCACAGAAA CATCCACAAT ACAAGAGATC AGTGGGGAAA CATCCGCATT TCCTGAAATT 
AGAATAGAAA CATCCACCTT TCAAGAAATC AGTGGGGAAA CGTCCGCATT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGCGGCGAAA CGTCTGCGTT TCCCGAGATT 
ACCATAGAAG CCTCCACAGT CCATGAAACC AGTGGAGAAA CATCTGCCTT TCCCGAAATT 
AGCATAGAAA CATCCACAGT CCATGAAATC AGTGGGGAAA GTTCTGCCTT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGGGGTGAAA CATCTGCGTT CCCTGAGATT 
ACCATAGAAG CCTCCACCAT TCAAGAGATC AGTGGAGAAA CATCTGCATT TCCTGAAATT 
AGCATAGCAA CTTCCACCGT CCGTGAAATC AGCGGCGAAA CGTCTGCCTT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGGGGTGAAA CATCTGCATT GCCTGAGATT 
ACCGTAGAGA CATCCACTGT CCATGAAACC AGTGGGGAAG CGTCTGCCTT TCCTGAAATT 
AGCATAGAAA CTTCCACAAG ACAAGAAGCC AGGGGTGAAG CATCCGCCTA CCCTGAGGTT 
AGCATAGAGG CATCCACAAC TCAAGAAGTA AGTGGGGAAA GTTCTGCCTT CCCTGAAATT 
AGCGTAGAGA CATCCACAAG TCAGGAAGCC CGCGGTGAAA CATCTGCCTT TCCTGAGATT 
GGCATAGAGA CATCCACAGC CCACGAAGGC AGTGGGGAAA CTCCTGGGCT GCCTGCTGTT 
AGCACTGACA CTGCTGCCAC GTCTCTGGCC AGTGGTGAGC CCTCCGGTGC TCCTGAGAAG 
GAAACTCCCG ACACAACATC ACATCTGATC ACGGGCGTTT CAGGGGAAAC CTCTGTCCCA 
GATGCTGTAA TCAGTACCAG TGCTCCAGAT GTTGAACTAG CGCAGGGACC CAGAAACACT 
GAAGAGACTC AGCTTGAAAT AGAGCCCTCC ACTCCTGCGG CATCTGGACA AGAGACAGAA 
ACAGCTGCTG TCCTCGACAA TCCCCATCTG CCAGCCACTG CTACTGCTGC CCTGCATCCA 
GCCTCCCAAG AAGCAGTAGA TGCACTGGGA CCCACGACAG AAGACACTGA TGAGTGCCAC 
TCAAGCCCCT GTCTGAATGG AGCTACCTGC GTTGATGGCA TCGACTCTTT CAAATGCTTA 
TGCCTTCCCA GCTACGGAGG GGACCTGTGT GAGATCGACC TGGCAAACTG TGAGGAAGGC 
TGGATCAAGT TCCAGGGCCA CTGCTACAGG CACTTTGAAG AGAGGGAGAC GTGGATGGAC 
GCAGAGTCCA GGTGCAGAGA ACATCAAGCC CACCTGAGCA GCATCATTAC TCCAGAGGAA 
CAAGAATTTG TGAACAGCCA TGCACAAGAC TATCAGTGGA TCGGCCTCAG TGACAGAGCC 
GTGGAGAATG ATTTCCGCTG GTCTGATGGA CACTCGCTGC AATTTGAGAA CTGGCGGCCC 
AACCAACCTG ACAACTTCTT CTCCGCGGGC GAGGACTGCG TTGTGATGAT CTGGCATGAG 
CAAGGCGAAT GGAACGACGT CCCCTGCAAC TATCACCTGC CTTTCACGTG CAAGAAGGGA 
ACAGTTGCCT GTGGGGACCC ACCTGTAGTG GAGAACGCGC GGACCTTTGG CCGGAAGAAG 
GACCGCTATG AGATCAACTC CCTGGTGCGG TACCAGTGTG ACCACGGCTA CATCCAGCGC 
CACGTGCCCA CCATCCGCTG CCAACCCAAC GGGCACTGGG AGGAACCGCG GATATCCTGC 
ACAAACCCCT CCAGCTACCA ACGCCGGCTA TACAAGAGGA GCCCCCGGAG CCGGTTGAGA 
CCCGGTGTCG TGCACAGACC CACCCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015147463.1
CDS	1393..7662
Translation	MTTLLLVFVCLQAITTAASAELSDSSDGLEVKIPEQSPLRVVLGSSLNIPCYFNIPEEEDTNALLTPRIKWSKLSNGTEIVLLVATGGKIRLNAEYREVISLPNYPAIPTDATLEIKALRSNHTGIYRCEVMYGIEDRQDTIEVLVKGVVFHYRAISTRYTLNFERAKQACIQNSAVIATPEQLQAAYEDGYEQCDAGWLADQTVRYPIHLPRERCYGDKDEFPGVRTYGVRETDETYDVYCYAEQMQGKVFYATSPEKFTFQEAFDKCHSLGARLATTGELYLAWKDGMDMCSAGWLADRSVRYPISRARPNCGGNLVGVRTVYLNPANQTGYPHPSSRYDAICYSGDDFEALVPGLFTDEVGTELGSAFTIQTVTQTEVELPLPRNVTEEEARGSIATLEPMEITATATELYEAFTVLPDLFATSVTVETASPREENVTREEITGIWAVPEEVTTSVSGTAFTTGMAEVSSVEEAIAVTATPGLESASPFTIEDHLVQVTAAPDVALLPRQPISPTGVVFHYRAATSRYAFSFIQAQQACLENNAVIATPEQLQAAYEAGFDQCDAGWLRDQTVRYPIVNPRSNCVGDKESSPGVRSYGMRPASETYDVYCYIDRLKGEVFFATQPEQFTFQEAQLYCESQNATLASAGQLHAAWKQGLDRCYPGWLADGSLRYPIVSPRPACGGDAPGVRTIYQHHNQTGFPDPLSRHHAFCFRALPSVVEEGVTSLFEEEVMVTQLIPGVEGIPSGEETTVETELSSEPENQTAQGTEVFPTDVSLLSARPSAFPPATVIPEETSTNASIPEVSGEFPESGEHPTSGEPSASGAPDTSGEPTSVGFELSGEQSGIGESGLPSVDLQSSGFVPGESGLPSGDVSGLPSGIVDISGLPSAEEEVTVSVSRIPEVSGMPSGAESSGLHSGFSGEISGTELISGLPSGEESGLASGFPTISLVDSTLVEVVTAAPGRQEEGKGSIGVSGEEELSGFPSAEWDSSGARGLPSGAETSGEQSGVPELSGEHSGVPGLSGEPFEVPELSGEHSGVTELSGEHSGLPELSGEPFGVPELSGFPSGLDISGEPSGAPEVSGPVDVSGLTSGVDGSGEVSGVTFISTSLQEVTTQSVAEAEAKEILEISGLPSGETSGMVSGSLDVSGQPSGHIDFGGSASGVLEMSGFPSGAVESSGEASGVEVTSVLASGEESGLTSGFPTVSLVDTTLVEVVTQTSVAQEVGEGPSGMIEISGFLSGDRGVSGEGSGAVQSSGLPSGTGDFSGEPSGIPYFSGDISGATDLSGQPSAVTDISGEDSGLPEVTLVTSDLVEVVTRPTVSQELGGETAVTFPYVFGPSGEGSASGDLSGGASAEGGIETSAAYEISGESSAFPETSIETSTDQEISGEASAYPEISVETSTHLETSGETSAYPEISTETSTIQEVSGETSAFPEISTETSTIQEISGETSAFPEIRIETSTFQEISGETSAFPEIRIETSTSQEARGETSAFPEITIEASTVHETSGETSAFPEISIETSTVHEISGESSAFPEIRIETSTSQEARGETSAFPEITIEASTIQEISGETSAFPEISIATSTVREISGETSAFPEIRIETSTSQEARGETSALPEITVETSTVHETSGEASAFPEISIETSTRQEARGEASAYPEVSIEASTTQEVSGESSAFPEISVETSTSQEARGETSAFPEIGIETSTAHEGSGETPGLPAVSTDTAATSLASGEPSGAPEKETPDTTSHLITGVSGETSVPDAVISTSAPDVELAQGPRNTEETQLEIEPSTPAASGQETETAAVLDNPHLPATATAALHPASQEAVDALGPTTEDTDECHSSPCLNGATCVDGIDSFKCLCLPSYGGDLCEIDLANCEEGWIKFQGHCYRHFEERETWMDAESRCREHQAHLSSIITPEEQEFVNSHAQDYQWIGLSDRAVENDFRWSDGHSLQFENWRPNQPDNFFSAGEDCVVMIWHEQGEWNDVPCNYHLPFTCKKGTVACGDPPVVENARTFGRKKDRYEINSLVRYQCDHGYIQRHVPTIRCQPNGHWEEPRISCTNPSSYQRRLYKRSPRSRLRPGVVHRPTH

Target ORF information:

RefSeq Version	XM_015291977.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus aggrecan (ACAN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291977.2

ORF Insert Sequence:

ATGACCACTC TACTACTAGT GTTTGTGTGT TTGCAAGCCA TCACCACAGC TGCCTCCGCA 
GAGCTCTCAG ACAGTAGCGA TGGCCTGGAA GTGAAGATAC CTGAGCAGTC TCCCCTGCGT 
GTTGTCCTGG GAAGCTCCCT GAACATCCCC TGCTATTTCA ACATCCCAGA GGAAGAGGAC 
ACCAATGCTC TGCTGACCCC CCGGATCAAA TGGAGCAAGC TTTCCAATGG AACAGAGATT 
GTCTTACTAG TGGCCACCGG TGGGAAGATC CGGCTCAATG CAGAGTACAG AGAGGTGATC 
TCCTTGCCCA ATTACCCTGC CATCCCCACT GATGCCACCT TGGAAATCAA GGCGCTGAGA 
TCCAACCACA CTGGGATTTA TCGCTGTGAA GTGATGTATG GGATTGAGGA CAGACAAGAC 
ACCATAGAGG TCCTGGTGAA AGGCGTCGTG TTCCACTACA GAGCAATCTC CACAAGGTAC 
ACTTTGAACT TCGAGAGGGC AAAGCAGGCC TGTATCCAGA ACAGTGCTGT CATTGCCACC 
CCTGAGCAGC TGCAGGCTGC CTACGAGGAT GGGTACGAGC AGTGCGATGC CGGCTGGCTG 
GCTGATCAGA CTGTCAGGTA CCCCATCCAT CTGCCCCGGG AGCGCTGCTA CGGTGACAAG 
GATGAGTTTC CAGGAGTGAG AACCTACGGT GTCCGTGAGA CAGATGAAAC CTATGATGTT 
TACTGCTATG CAGAGCAAAT GCAAGGCAAA GTCTTCTACG CCACCTCCCC CGAGAAGTTC 
ACCTTCCAGG AAGCTTTTGA CAAATGCCAC AGCTTGGGAG CCCGCCTGGC CACCACGGGC 
GAGCTGTACT TGGCCTGGAA GGATGGCATG GACATGTGCA GTGCGGGCTG GCTGGCTGAC 
CGCAGCGTTC GCTACCCCAT TTCCAGAGCA CGGCCCAATT GTGGAGGGAA CCTGGTGGGC 
GTGCGGACCG TTTACCTGAA CCCTGCCAAC CAGACAGGGT ACCCTCACCC CAGCTCACGC 
TACGATGCCA TCTGTTACAG TGGTGATGAC TTCGAGGCTC TGGTCCCAGG GCTGTTCACT 
GACGAGGTTG GGACTGAGCT GGGCAGTGCT TTTACCATAC AGACCGTCAC ACAGACTGAG 
GTGGAGCTGC CCCTGCCACG CAATGTCACA GAAGAGGAGG CCCGTGGCAG CATCGCTACC 
CTGGAGCCCA TGGAGATCAC AGCCACCGCC ACTGAGCTGT ATGAGGCCTT CACCGTCCTG 
CCTGACCTCT TTGCCACCAG TGTCACAGTA GAGACAGCTT CCCCAAGGGA AGAGAACGTG 
ACCAGAGAGG AGATCACAGG GATATGGGCT GTGCCTGAAG AGGTCACTAC ATCGGTCTCA 
GGCACTGCTT TCACCACCGG AATGGCAGAG GTGAGCTCAG TGGAAGAGGC CATAGCAGTG 
ACTGCCACAC CAGGACTGGA GTCTGCCTCT CCATTCACCA TAGAAGATCA TCTCGTGCAA 
GTGACAGCAG CCCCTGATGT TGCCCTCCTC CCCAGGCAGC CCATTTCCCC CACTGGTGTG 
GTGTTCCACT ACCGTGCTGC CACCAGCAGA TACGCCTTCT CCTTCATCCA AGCCCAGCAG 
GCCTGCCTGG AGAACAATGC CGTTATTGCC ACTCCCGAGC AGCTCCAGGC TGCCTACGAG 
GCTGGCTTTG ATCAGTGCGA TGCCGGCTGG CTGCGGGACC AGACGGTCAG GTATCCCATT 
GTGAATCCCC GCAGCAACTG TGTAGGAGAC AAAGAGAGCT CCCCAGGTGT GCGGTCATAC 
GGCATGCGCC CGGCCTCAGA GACCTACGAT GTGTACTGTT ACATCGACAG GCTAAAGGGT 
GAGGTGTTTT TTGCCACCCA GCCAGAGCAG TTCACCTTCC AAGAAGCCCA GCTGTACTGT 
GAAAGCCAAA ATGCCACGCT GGCCTCTGCT GGGCAACTCC ACGCTGCCTG GAAGCAGGGC 
CTGGATAGGT GCTACCCTGG GTGGCTGGCC GACGGCAGCT TGCGGTACCC CATTGTGAGC 
CCCCGACCTG CCTGCGGGGG GGATGCACCT GGTGTGAGGA CCATCTACCA GCACCACAAC 
CAGACGGGCT TTCCTGACCC TCTGTCACGG CATCACGCTT TCTGCTTCAG AGCTCTGCCA 
TCCGTAGTGG AGGAGGGGGT GACCTCACTC TTTGAAGAAG AGGTGATGGT AACCCAACTG 
ATCCCTGGAG TGGAAGGAAT ACCTTCTGGG GAGGAAACAA CTGTGGAGAC AGAGCTTTCC 
TCTGAGCCTG AGAATCAGAC AGCCCAGGGA ACGGAGGTCT TCCCAACTGA CGTATCACTG 
CTCTCAGCGA GACCATCTGC TTTTCCTCCA GCTACTGTAA TACCAGAGGA AACAAGTACC 
AATGCTTCCA TCCCCGAAGT GTCCGGAGAG TTCCCTGAGT CTGGAGAGCA CCCAACCAGT 
GGCGAACCTT CAGCATCTGG AGCCCCAGAC ACGAGTGGAG AACCAACTTC CGTAGGTTTT 
GAACTGAGTG GAGAGCAATC CGGGATTGGA GAAAGTGGAT TACCATCTGT AGACCTGCAG 
AGCAGTGGCT TTGTACCTGG AGAAAGTGGC CTTCCCTCAG GGGATGTGAG CGGGTTGCCT 
TCTGGCATTG TTGATATCAG CGGCTTGCCT TCTGCAGAGG AAGAGGTAAC GGTGTCTGTT 
TCGAGGATAC CAGAAGTTAG TGGAATGCCA TCTGGAGCTG AAAGCAGTGG TCTGCATTCT 
GGATTTAGTG GAGAAATCTC TGGCACTGAG CTTATCAGTG GCCTGCCATC TGGAGAGGAA 
AGTGGGCTCG CCTCTGGTTT TCCCACCATC TCCCTCGTGG ATTCCACTTT GGTGGAGGTT 
GTAACGGCAG CACCGGGACG GCAAGAGGAG GGAAAAGGAT CAATTGGAGT CAGTGGTGAA 
GAAGAGCTGT CAGGGTTTCC GTCTGCAGAG TGGGACAGCA GTGGGGCCAG AGGGCTGCCC 
TCGGGAGCTG AAACCAGTGG GGAGCAATCC GGGGTGCCCG AGCTCAGTGG GGAGCATTCC 
GGGGTGCCTG GGCTCAGTGG AGAGCCTTTT GAGGTGCCTG AGCTCAGCGG GGAGCATTCC 
GGGGTGACTG AGCTCAGTGG GGAGCATTCT GGGCTTCCTG AGCTCAGTGG AGAGCCTTTT 
GGGGTGCCTG AGCTCAGCGG GTTTCCCTCC GGACTGGACA TCAGTGGGGA ACCATCTGGA 
GCACCCGAGG TGAGCGGCCC GGTGGACGTG AGCGGCCTTA CCTCTGGTGT TGATGGAAGT 
GGTGAGGTCT CAGGCGTTAC CTTTATAAGC ACCAGCCTGC AAGAAGTGAC AACCCAGTCA 
GTTGCAGAAG CAGAAGCAAA AGAAATTCTA GAGATCAGTG GACTGCCTTC AGGAGAGACA 
TCAGGCATGG TGTCTGGGAG CTTAGATGTC AGCGGTCAGC CTTCGGGACA CATAGACTTT 
GGTGGGAGCG CTTCTGGAGT GCTGGAGATG AGCGGATTTC CAAGCGGAGC GGTTGAGAGC 
AGTGGAGAAG CCTCTGGGGT TGAAGTCACC AGTGTCCTCG CGTCTGGAGA GGAAAGTGGG 
CTCACCTCAG GCTTTCCCAC CGTGTCTCTC GTGGATACCA CGTTGGTAGA AGTTGTAACG 
CAGACATCAG TTGCTCAAGA GGTGGGAGAA GGACCATCTG GGATGATAGA AATCAGTGGA 
TTTCTTTCTG GAGACAGAGG AGTATCTGGA GAAGGGTCTG GAGCTGTGCA GTCTAGTGGG 
CTTCCTTCAG GAACAGGAGA CTTCAGCGGA GAGCCATCCG GGATCCCGTA TTTCAGCGGA 
GACATTTCTG GAGCCACAGA TCTAAGCGGA CAACCTTCCG CAGTGACTGA TATTAGTGGG 
GAGGACTCGG GACTTCCAGA AGTCACTTTA GTCACATCCG ATTTAGTAGA AGTTGTGACA 
AGGCCAACAG TTTCACAGGA GCTGGGTGGG GAAACAGCTG TGACGTTTCC CTATGTCTTT 
GGGCCAAGTG GTGAGGGCTC TGCATCTGGA GACCTGAGTG GGGGAGCATC TGCAGAAGGT 
GGTATAGAAA CATCAGCAGC TTATGAAATC AGTGGTGAGA GCTCTGCCTT CCCTGAAACG 
AGTATAGAAA CATCCACAGA TCAAGAAATC AGTGGGGAAG CATCTGCATA CCCTGAGATT 
AGCGTAGAAA CATCCACGCA TCTGGAAACC AGTGGGGAAA CATCTGCATA CCCTGAGATT 
AGCACAGAGA CGTCCACCAT TCAGGAAGTT AGTGGGGAAA CGTCTGCCTT TCCTGAAATT 
AGCACAGAAA CATCCACAAT ACAAGAGATC AGTGGGGAAA CATCCGCATT TCCTGAAATT 
AGAATAGAAA CATCCACCTT TCAAGAAATC AGTGGGGAAA CGTCCGCATT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGCGGCGAAA CGTCTGCGTT TCCCGAGATT 
ACCATAGAAG CCTCCACAGT CCATGAAACC AGTGGAGAAA CATCTGCCTT TCCCGAAATT 
AGCATAGAAA CATCCACAGT CCATGAAATC AGTGGGGAAA GTTCTGCCTT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGGGGTGAAA CATCTGCGTT CCCTGAGATT 
ACCATAGAAG CCTCCACCAT TCAAGAGATC AGTGGAGAAA CATCTGCATT TCCTGAAATT 
AGCATAGCAA CTTCCACCGT CCGTGAAATC AGCGGCGAAA CGTCTGCCTT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGGGGTGAAA CATCTGCATT GCCTGAGATT 
ACCGTAGAGA CATCCACTGT CCATGAAACC AGTGGGGAAG CGTCTGCCTT TCCTGAAATT 
AGCATAGAAA CTTCCACAAG ACAAGAAGCC AGGGGTGAAG CATCCGCCTA CCCTGAGGTT 
AGCATAGAGG CATCCACAAC TCAAGAAGTA AGTGGGGAAA GTTCTGCCTT CCCTGAAATT 
AGCGTAGAGA CATCCACAAG TCAGGAAGCC CGCGGTGAAA CATCTGCCTT TCCTGAGATT 
GGCATAGAGA CATCCACAGC CCACGAAGGC AGTGGGGAAA CTCCTGGGCT GCCTGCTGTT 
AGCACTGACA CTGCTGCCAC GTCTCTGGCC AGTGGTGAGC CCTCCGGTGC TCCTGAGAAG 
GAAACTCCCG ACACAACATC ACATCTGATC ACGGGCGTTT CAGGGGAAAC CTCTGTCCCA 
GATGCTGTAA TCAGTACCAG TGCTCCAGAT GTTGAACTAG CGCAGGGACC CAGAAACACT 
GAAGAGACTC AGCTTGAAAT AGAGCCCTCC ACTCCTGCGG CATCTGGACA AGAGACAGAA 
ACAGCTGCTG TCCTCGACAA TCCCCATCTG CCAGCCACTG CTACTGCTGC CCTGCATCCA 
GCCTCCCAAG AAGCAGTAGA TGCACTGGGA CCCACGACAG AAGACACTGA TGAGTGCCAC 
TCAAGCCCCT GTCTGAATGG AGCTACCTGC GTTGATGGCA TCGACTCTTT CAAATGCTTA 
TGCCTTCCCA GCTACGGAGG GGACCTGTGT GAGATCGACC TGGCAAACTG TGAGGAAGGC 
TGGATCAAGT TCCAGGGCCA CTGCTACAGG CACTTTGAAG AGAGGGAGAC GTGGATGGAC 
GCAGAGTCCA GGTGCAGAGA ACATCAAGCC CACCTGAGCA GCATCATTAC TCCAGAGGAA 
CAAGAATTTG TGAACAGCCA TGCACAAGAC TATCAGTGGA TCGGCCTCAG TGACAGAGCC 
GTGGAGAATG ATTTCCGCTG GTCTGATGGA CACTCGCTGC AATTTGAGAA CTGGCGGCCC 
AACCAACCTG ACAACTTCTT CTCCGCGGGC GAGGACTGCG TTGTGATGAT CTGGCATGAG 
CAAGGCGAAT GGAACGACGT CCCCTGCAAC TATCACCTGC CTTTCACGTG CAAGAAGGGA 
ACAGTTGCCT GTGGGGACCC ACCTGTAGTG GAGAACGCGC GGACCTTTGG CCGGAAGAAG 
GACCGCTATG AGATCAACTC CCTGGTGCGG TACCAGTGTG ACCACGGCTA CATCCAGCGC 
CACGTGCCCA CCATCCGCTG CCAACCCAAC GGGCACTGGG AGGAACCGCG GATATCCTGC 
ACAAACCCCT CCAGCTACCA ACGCCGGCTA TACAAGAGGA GCCCCCGGAG CCGGTTGAGA 
CCCGGTGTCG TGCACAGACC CACCCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACAN ( XM_015291978.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015147464.1 Gallus gallus aggrecan (ACAN), transcript variant X2, mRNA.

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CloneID	OGa28230
Clone ID Related Accession (Same CDS sequence)	XM_015291977.1 , XM_015291977.2 , XM_015291978.2 , NM_204955.2 , XM_015291978.1
Accession Version	XM_015291978.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	aggrecan core protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006097.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291978.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACTC TACTACTAGT GTTTGTGTGT TTGCAAGCCA TCACCACAGC TGCCTCCGCA 
GAGCTCTCAG ACAGTAGCGA TGGCCTGGAA GTGAAGATAC CTGAGCAGTC TCCCCTGCGT 
GTTGTCCTGG GAAGCTCCCT GAACATCCCC TGCTATTTCA ACATCCCAGA GGAAGAGGAC 
ACCAATGCTC TGCTGACCCC CCGGATCAAA TGGAGCAAGC TTTCCAATGG AACAGAGATT 
GTCTTACTAG TGGCCACCGG TGGGAAGATC CGGCTCAATG CAGAGTACAG AGAGGTGATC 
TCCTTGCCCA ATTACCCTGC CATCCCCACT GATGCCACCT TGGAAATCAA GGCGCTGAGA 
TCCAACCACA CTGGGATTTA TCGCTGTGAA GTGATGTATG GGATTGAGGA CAGACAAGAC 
ACCATAGAGG TCCTGGTGAA AGGCGTCGTG TTCCACTACA GAGCAATCTC CACAAGGTAC 
ACTTTGAACT TCGAGAGGGC AAAGCAGGCC TGTATCCAGA ACAGTGCTGT CATTGCCACC 
CCTGAGCAGC TGCAGGCTGC CTACGAGGAT GGGTACGAGC AGTGCGATGC CGGCTGGCTG 
GCTGATCAGA CTGTCAGGTA CCCCATCCAT CTGCCCCGGG AGCGCTGCTA CGGTGACAAG 
GATGAGTTTC CAGGAGTGAG AACCTACGGT GTCCGTGAGA CAGATGAAAC CTATGATGTT 
TACTGCTATG CAGAGCAAAT GCAAGGCAAA GTCTTCTACG CCACCTCCCC CGAGAAGTTC 
ACCTTCCAGG AAGCTTTTGA CAAATGCCAC AGCTTGGGAG CCCGCCTGGC CACCACGGGC 
GAGCTGTACT TGGCCTGGAA GGATGGCATG GACATGTGCA GTGCGGGCTG GCTGGCTGAC 
CGCAGCGTTC GCTACCCCAT TTCCAGAGCA CGGCCCAATT GTGGAGGGAA CCTGGTGGGC 
GTGCGGACCG TTTACCTGAA CCCTGCCAAC CAGACAGGGT ACCCTCACCC CAGCTCACGC 
TACGATGCCA TCTGTTACAG TGGTGATGAC TTCGAGGCTC TGGTCCCAGG GCTGTTCACT 
GACGAGGTTG GGACTGAGCT GGGCAGTGCT TTTACCATAC AGACCGTCAC ACAGACTGAG 
GTGGAGCTGC CCCTGCCACG CAATGTCACA GAAGAGGAGG CCCGTGGCAG CATCGCTACC 
CTGGAGCCCA TGGAGATCAC AGCCACCGCC ACTGAGCTGT ATGAGGCCTT CACCGTCCTG 
CCTGACCTCT TTGCCACCAG TGTCACAGTA GAGACAGCTT CCCCAAGGGA AGAGAACGTG 
ACCAGAGAGG AGATCACAGG GATATGGGCT GTGCCTGAAG AGGTCACTAC ATCGGTCTCA 
GGCACTGCTT TCACCACCGG AATGGCAGAG GTGAGCTCAG TGGAAGAGGC CATAGCAGTG 
ACTGCCACAC CAGGACTGGA GTCTGCCTCT CCATTCACCA TAGAAGATCA TCTCGTGCAA 
GTGACAGCAG CCCCTGATGT TGCCCTCCTC CCCAGGCAGC CCATTTCCCC CACTGGTGTG 
GTGTTCCACT ACCGTGCTGC CACCAGCAGA TACGCCTTCT CCTTCATCCA AGCCCAGCAG 
GCCTGCCTGG AGAACAATGC CGTTATTGCC ACTCCCGAGC AGCTCCAGGC TGCCTACGAG 
GCTGGCTTTG ATCAGTGCGA TGCCGGCTGG CTGCGGGACC AGACGGTCAG GTATCCCATT 
GTGAATCCCC GCAGCAACTG TGTAGGAGAC AAAGAGAGCT CCCCAGGTGT GCGGTCATAC 
GGCATGCGCC CGGCCTCAGA GACCTACGAT GTGTACTGTT ACATCGACAG GCTAAAGGGT 
GAGGTGTTTT TTGCCACCCA GCCAGAGCAG TTCACCTTCC AAGAAGCCCA GCTGTACTGT 
GAAAGCCAAA ATGCCACGCT GGCCTCTGCT GGGCAACTCC ACGCTGCCTG GAAGCAGGGC 
CTGGATAGGT GCTACCCTGG GTGGCTGGCC GACGGCAGCT TGCGGTACCC CATTGTGAGC 
CCCCGACCTG CCTGCGGGGG GGATGCACCT GGTGTGAGGA CCATCTACCA GCACCACAAC 
CAGACGGGCT TTCCTGACCC TCTGTCACGG CATCACGCTT TCTGCTTCAG AGCTCTGCCA 
TCCGTAGTGG AGGAGGGGGT GACCTCACTC TTTGAAGAAG AGGTGATGGT AACCCAACTG 
ATCCCTGGAG TGGAAGGAAT ACCTTCTGGG GAGGAAACAA CTGTGGAGAC AGAGCTTTCC 
TCTGAGCCTG AGAATCAGAC AGCCCAGGGA ACGGAGGTCT TCCCAACTGA CGTATCACTG 
CTCTCAGCGA GACCATCTGC TTTTCCTCCA GCTACTGTAA TACCAGAGGA AACAAGTACC 
AATGCTTCCA TCCCCGAAGT GTCCGGAGAG TTCCCTGAGT CTGGAGAGCA CCCAACCAGT 
GGCGAACCTT CAGCATCTGG AGCCCCAGAC ACGAGTGGAG AACCAACTTC CGTAGGTTTT 
GAACTGAGTG GAGAGCAATC CGGGATTGGA GAAAGTGGAT TACCATCTGT AGACCTGCAG 
AGCAGTGGCT TTGTACCTGG AGAAAGTGGC CTTCCCTCAG GGGATGTGAG CGGGTTGCCT 
TCTGGCATTG TTGATATCAG CGGCTTGCCT TCTGCAGAGG AAGAGGTAAC GGTGTCTGTT 
TCGAGGATAC CAGAAGTTAG TGGAATGCCA TCTGGAGCTG AAAGCAGTGG TCTGCATTCT 
GGATTTAGTG GAGAAATCTC TGGCACTGAG CTTATCAGTG GCCTGCCATC TGGAGAGGAA 
AGTGGGCTCG CCTCTGGTTT TCCCACCATC TCCCTCGTGG ATTCCACTTT GGTGGAGGTT 
GTAACGGCAG CACCGGGACG GCAAGAGGAG GGAAAAGGAT CAATTGGAGT CAGTGGTGAA 
GAAGAGCTGT CAGGGTTTCC GTCTGCAGAG TGGGACAGCA GTGGGGCCAG AGGGCTGCCC 
TCGGGAGCTG AAACCAGTGG GGAGCAATCC GGGGTGCCCG AGCTCAGTGG GGAGCATTCC 
GGGGTGCCTG GGCTCAGTGG AGAGCCTTTT GAGGTGCCTG AGCTCAGCGG GGAGCATTCC 
GGGGTGACTG AGCTCAGTGG GGAGCATTCT GGGCTTCCTG AGCTCAGTGG AGAGCCTTTT 
GGGGTGCCTG AGCTCAGCGG GTTTCCCTCC GGACTGGACA TCAGTGGGGA ACCATCTGGA 
GCACCCGAGG TGAGCGGCCC GGTGGACGTG AGCGGCCTTA CCTCTGGTGT TGATGGAAGT 
GGTGAGGTCT CAGGCGTTAC CTTTATAAGC ACCAGCCTGC AAGAAGTGAC AACCCAGTCA 
GTTGCAGAAG CAGAAGCAAA AGAAATTCTA GAGATCAGTG GACTGCCTTC AGGAGAGACA 
TCAGGCATGG TGTCTGGGAG CTTAGATGTC AGCGGTCAGC CTTCGGGACA CATAGACTTT 
GGTGGGAGCG CTTCTGGAGT GCTGGAGATG AGCGGATTTC CAAGCGGAGC GGTTGAGAGC 
AGTGGAGAAG CCTCTGGGGT TGAAGTCACC AGTGTCCTCG CGTCTGGAGA GGAAAGTGGG 
CTCACCTCAG GCTTTCCCAC CGTGTCTCTC GTGGATACCA CGTTGGTAGA AGTTGTAACG 
CAGACATCAG TTGCTCAAGA GGTGGGAGAA GGACCATCTG GGATGATAGA AATCAGTGGA 
TTTCTTTCTG GAGACAGAGG AGTATCTGGA GAAGGGTCTG GAGCTGTGCA GTCTAGTGGG 
CTTCCTTCAG GAACAGGAGA CTTCAGCGGA GAGCCATCCG GGATCCCGTA TTTCAGCGGA 
GACATTTCTG GAGCCACAGA TCTAAGCGGA CAACCTTCCG CAGTGACTGA TATTAGTGGG 
GAGGACTCGG GACTTCCAGA AGTCACTTTA GTCACATCCG ATTTAGTAGA AGTTGTGACA 
AGGCCAACAG TTTCACAGGA GCTGGGTGGG GAAACAGCTG TGACGTTTCC CTATGTCTTT 
GGGCCAAGTG GTGAGGGCTC TGCATCTGGA GACCTGAGTG GGGGAGCATC TGCAGAAGGT 
GGTATAGAAA CATCAGCAGC TTATGAAATC AGTGGTGAGA GCTCTGCCTT CCCTGAAACG 
AGTATAGAAA CATCCACAGA TCAAGAAATC AGTGGGGAAG CATCTGCATA CCCTGAGATT 
AGCGTAGAAA CATCCACGCA TCTGGAAACC AGTGGGGAAA CATCTGCATA CCCTGAGATT 
AGCACAGAGA CGTCCACCAT TCAGGAAGTT AGTGGGGAAA CGTCTGCCTT TCCTGAAATT 
AGCACAGAAA CATCCACAAT ACAAGAGATC AGTGGGGAAA CATCCGCATT TCCTGAAATT 
AGAATAGAAA CATCCACCTT TCAAGAAATC AGTGGGGAAA CGTCCGCATT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGCGGCGAAA CGTCTGCGTT TCCCGAGATT 
ACCATAGAAG CCTCCACAGT CCATGAAACC AGTGGAGAAA CATCTGCCTT TCCCGAAATT 
AGCATAGAAA CATCCACAGT CCATGAAATC AGTGGGGAAA GTTCTGCCTT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGGGGTGAAA CATCTGCGTT CCCTGAGATT 
ACCATAGAAG CCTCCACCAT TCAAGAGATC AGTGGAGAAA CATCTGCATT TCCTGAAATT 
AGCATAGCAA CTTCCACCGT CCGTGAAATC AGCGGCGAAA CGTCTGCCTT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGGGGTGAAA CATCTGCATT GCCTGAGATT 
ACCGTAGAGA CATCCACTGT CCATGAAACC AGTGGGGAAG CGTCTGCCTT TCCTGAAATT 
AGCATAGAAA CTTCCACAAG ACAAGAAGCC AGGGGTGAAG CATCCGCCTA CCCTGAGGTT 
AGCATAGAGG CATCCACAAC TCAAGAAGTA AGTGGGGAAA GTTCTGCCTT CCCTGAAATT 
AGCGTAGAGA CATCCACAAG TCAGGAAGCC CGCGGTGAAA CATCTGCCTT TCCTGAGATT 
GGCATAGAGA CATCCACAGC CCACGAAGGC AGTGGGGAAA CTCCTGGGCT GCCTGCTGTT 
AGCACTGACA CTGCTGCCAC GTCTCTGGCC AGTGGTGAGC CCTCCGGTGC TCCTGAGAAG 
GAAACTCCCG ACACAACATC ACATCTGATC ACGGGCGTTT CAGGGGAAAC CTCTGTCCCA 
GATGCTGTAA TCAGTACCAG TGCTCCAGAT GTTGAACTAG CGCAGGGACC CAGAAACACT 
GAAGAGACTC AGCTTGAAAT AGAGCCCTCC ACTCCTGCGG CATCTGGACA AGAGACAGAA 
ACAGCTGCTG TCCTCGACAA TCCCCATCTG CCAGCCACTG CTACTGCTGC CCTGCATCCA 
GCCTCCCAAG AAGCAGTAGA TGCACTGGGA CCCACGACAG AAGACACTGA TGAGTGCCAC 
TCAAGCCCCT GTCTGAATGG AGCTACCTGC GTTGATGGCA TCGACTCTTT CAAATGCTTA 
TGCCTTCCCA GCTACGGAGG GGACCTGTGT GAGATCGACC TGGCAAACTG TGAGGAAGGC 
TGGATCAAGT TCCAGGGCCA CTGCTACAGG CACTTTGAAG AGAGGGAGAC GTGGATGGAC 
GCAGAGTCCA GGTGCAGAGA ACATCAAGCC CACCTGAGCA GCATCATTAC TCCAGAGGAA 
CAAGAATTTG TGAACAGCCA TGCACAAGAC TATCAGTGGA TCGGCCTCAG TGACAGAGCC 
GTGGAGAATG ATTTCCGCTG GTCTGATGGA CACTCGCTGC AATTTGAGAA CTGGCGGCCC 
AACCAACCTG ACAACTTCTT CTCCGCGGGC GAGGACTGCG TTGTGATGAT CTGGCATGAG 
CAAGGCGAAT GGAACGACGT CCCCTGCAAC TATCACCTGC CTTTCACGTG CAAGAAGGGA 
ACAGTTGCCT GTGGGGACCC ACCTGTAGTG GAGAACGCGC GGACCTTTGG CCGGAAGAAG 
GACCGCTATG AGATCAACTC CCTGGTGCGG TACCAGTGTG ACCACGGCTA CATCCAGCGC 
CACGTGCCCA CCATCCGCTG CCAACCCAAC GGGCACTGGG AGGAACCGCG GATATCCTGC 
ACAAACCCCT CCAGCTACCA ACGCCGGCTA TACAAGAGGA GCCCCCGGAG CCGGTTGAGA 
CCCGGTGTCG TGCACAGACC CACCCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015147464.1
CDS	1494..7763
Translation	MTTLLLVFVCLQAITTAASAELSDSSDGLEVKIPEQSPLRVVLGSSLNIPCYFNIPEEEDTNALLTPRIKWSKLSNGTEIVLLVATGGKIRLNAEYREVISLPNYPAIPTDATLEIKALRSNHTGIYRCEVMYGIEDRQDTIEVLVKGVVFHYRAISTRYTLNFERAKQACIQNSAVIATPEQLQAAYEDGYEQCDAGWLADQTVRYPIHLPRERCYGDKDEFPGVRTYGVRETDETYDVYCYAEQMQGKVFYATSPEKFTFQEAFDKCHSLGARLATTGELYLAWKDGMDMCSAGWLADRSVRYPISRARPNCGGNLVGVRTVYLNPANQTGYPHPSSRYDAICYSGDDFEALVPGLFTDEVGTELGSAFTIQTVTQTEVELPLPRNVTEEEARGSIATLEPMEITATATELYEAFTVLPDLFATSVTVETASPREENVTREEITGIWAVPEEVTTSVSGTAFTTGMAEVSSVEEAIAVTATPGLESASPFTIEDHLVQVTAAPDVALLPRQPISPTGVVFHYRAATSRYAFSFIQAQQACLENNAVIATPEQLQAAYEAGFDQCDAGWLRDQTVRYPIVNPRSNCVGDKESSPGVRSYGMRPASETYDVYCYIDRLKGEVFFATQPEQFTFQEAQLYCESQNATLASAGQLHAAWKQGLDRCYPGWLADGSLRYPIVSPRPACGGDAPGVRTIYQHHNQTGFPDPLSRHHAFCFRALPSVVEEGVTSLFEEEVMVTQLIPGVEGIPSGEETTVETELSSEPENQTAQGTEVFPTDVSLLSARPSAFPPATVIPEETSTNASIPEVSGEFPESGEHPTSGEPSASGAPDTSGEPTSVGFELSGEQSGIGESGLPSVDLQSSGFVPGESGLPSGDVSGLPSGIVDISGLPSAEEEVTVSVSRIPEVSGMPSGAESSGLHSGFSGEISGTELISGLPSGEESGLASGFPTISLVDSTLVEVVTAAPGRQEEGKGSIGVSGEEELSGFPSAEWDSSGARGLPSGAETSGEQSGVPELSGEHSGVPGLSGEPFEVPELSGEHSGVTELSGEHSGLPELSGEPFGVPELSGFPSGLDISGEPSGAPEVSGPVDVSGLTSGVDGSGEVSGVTFISTSLQEVTTQSVAEAEAKEILEISGLPSGETSGMVSGSLDVSGQPSGHIDFGGSASGVLEMSGFPSGAVESSGEASGVEVTSVLASGEESGLTSGFPTVSLVDTTLVEVVTQTSVAQEVGEGPSGMIEISGFLSGDRGVSGEGSGAVQSSGLPSGTGDFSGEPSGIPYFSGDISGATDLSGQPSAVTDISGEDSGLPEVTLVTSDLVEVVTRPTVSQELGGETAVTFPYVFGPSGEGSASGDLSGGASAEGGIETSAAYEISGESSAFPETSIETSTDQEISGEASAYPEISVETSTHLETSGETSAYPEISTETSTIQEVSGETSAFPEISTETSTIQEISGETSAFPEIRIETSTFQEISGETSAFPEIRIETSTSQEARGETSAFPEITIEASTVHETSGETSAFPEISIETSTVHEISGESSAFPEIRIETSTSQEARGETSAFPEITIEASTIQEISGETSAFPEISIATSTVREISGETSAFPEIRIETSTSQEARGETSALPEITVETSTVHETSGEASAFPEISIETSTRQEARGEASAYPEVSIEASTTQEVSGESSAFPEISVETSTSQEARGETSAFPEIGIETSTAHEGSGETPGLPAVSTDTAATSLASGEPSGAPEKETPDTTSHLITGVSGETSVPDAVISTSAPDVELAQGPRNTEETQLEIEPSTPAASGQETETAAVLDNPHLPATATAALHPASQEAVDALGPTTEDTDECHSSPCLNGATCVDGIDSFKCLCLPSYGGDLCEIDLANCEEGWIKFQGHCYRHFEERETWMDAESRCREHQAHLSSIITPEEQEFVNSHAQDYQWIGLSDRAVENDFRWSDGHSLQFENWRPNQPDNFFSAGEDCVVMIWHEQGEWNDVPCNYHLPFTCKKGTVACGDPPVVENARTFGRKKDRYEINSLVRYQCDHGYIQRHVPTIRCQPNGHWEEPRISCTNPSSYQRRLYKRSPRSRLRPGVVHRPTH

Target ORF information:

RefSeq Version	XM_015291978.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus aggrecan (ACAN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291978.2

ORF Insert Sequence:

ATGACCACTC TACTACTAGT GTTTGTGTGT TTGCAAGCCA TCACCACAGC TGCCTCCGCA 
GAGCTCTCAG ACAGTAGCGA TGGCCTGGAA GTGAAGATAC CTGAGCAGTC TCCCCTGCGT 
GTTGTCCTGG GAAGCTCCCT GAACATCCCC TGCTATTTCA ACATCCCAGA GGAAGAGGAC 
ACCAATGCTC TGCTGACCCC CCGGATCAAA TGGAGCAAGC TTTCCAATGG AACAGAGATT 
GTCTTACTAG TGGCCACCGG TGGGAAGATC CGGCTCAATG CAGAGTACAG AGAGGTGATC 
TCCTTGCCCA ATTACCCTGC CATCCCCACT GATGCCACCT TGGAAATCAA GGCGCTGAGA 
TCCAACCACA CTGGGATTTA TCGCTGTGAA GTGATGTATG GGATTGAGGA CAGACAAGAC 
ACCATAGAGG TCCTGGTGAA AGGCGTCGTG TTCCACTACA GAGCAATCTC CACAAGGTAC 
ACTTTGAACT TCGAGAGGGC AAAGCAGGCC TGTATCCAGA ACAGTGCTGT CATTGCCACC 
CCTGAGCAGC TGCAGGCTGC CTACGAGGAT GGGTACGAGC AGTGCGATGC CGGCTGGCTG 
GCTGATCAGA CTGTCAGGTA CCCCATCCAT CTGCCCCGGG AGCGCTGCTA CGGTGACAAG 
GATGAGTTTC CAGGAGTGAG AACCTACGGT GTCCGTGAGA CAGATGAAAC CTATGATGTT 
TACTGCTATG CAGAGCAAAT GCAAGGCAAA GTCTTCTACG CCACCTCCCC CGAGAAGTTC 
ACCTTCCAGG AAGCTTTTGA CAAATGCCAC AGCTTGGGAG CCCGCCTGGC CACCACGGGC 
GAGCTGTACT TGGCCTGGAA GGATGGCATG GACATGTGCA GTGCGGGCTG GCTGGCTGAC 
CGCAGCGTTC GCTACCCCAT TTCCAGAGCA CGGCCCAATT GTGGAGGGAA CCTGGTGGGC 
GTGCGGACCG TTTACCTGAA CCCTGCCAAC CAGACAGGGT ACCCTCACCC CAGCTCACGC 
TACGATGCCA TCTGTTACAG TGGTGATGAC TTCGAGGCTC TGGTCCCAGG GCTGTTCACT 
GACGAGGTTG GGACTGAGCT GGGCAGTGCT TTTACCATAC AGACCGTCAC ACAGACTGAG 
GTGGAGCTGC CCCTGCCACG CAATGTCACA GAAGAGGAGG CCCGTGGCAG CATCGCTACC 
CTGGAGCCCA TGGAGATCAC AGCCACCGCC ACTGAGCTGT ATGAGGCCTT CACCGTCCTG 
CCTGACCTCT TTGCCACCAG TGTCACAGTA GAGACAGCTT CCCCAAGGGA AGAGAACGTG 
ACCAGAGAGG AGATCACAGG GATATGGGCT GTGCCTGAAG AGGTCACTAC ATCGGTCTCA 
GGCACTGCTT TCACCACCGG AATGGCAGAG GTGAGCTCAG TGGAAGAGGC CATAGCAGTG 
ACTGCCACAC CAGGACTGGA GTCTGCCTCT CCATTCACCA TAGAAGATCA TCTCGTGCAA 
GTGACAGCAG CCCCTGATGT TGCCCTCCTC CCCAGGCAGC CCATTTCCCC CACTGGTGTG 
GTGTTCCACT ACCGTGCTGC CACCAGCAGA TACGCCTTCT CCTTCATCCA AGCCCAGCAG 
GCCTGCCTGG AGAACAATGC CGTTATTGCC ACTCCCGAGC AGCTCCAGGC TGCCTACGAG 
GCTGGCTTTG ATCAGTGCGA TGCCGGCTGG CTGCGGGACC AGACGGTCAG GTATCCCATT 
GTGAATCCCC GCAGCAACTG TGTAGGAGAC AAAGAGAGCT CCCCAGGTGT GCGGTCATAC 
GGCATGCGCC CGGCCTCAGA GACCTACGAT GTGTACTGTT ACATCGACAG GCTAAAGGGT 
GAGGTGTTTT TTGCCACCCA GCCAGAGCAG TTCACCTTCC AAGAAGCCCA GCTGTACTGT 
GAAAGCCAAA ATGCCACGCT GGCCTCTGCT GGGCAACTCC ACGCTGCCTG GAAGCAGGGC 
CTGGATAGGT GCTACCCTGG GTGGCTGGCC GACGGCAGCT TGCGGTACCC CATTGTGAGC 
CCCCGACCTG CCTGCGGGGG GGATGCACCT GGTGTGAGGA CCATCTACCA GCACCACAAC 
CAGACGGGCT TTCCTGACCC TCTGTCACGG CATCACGCTT TCTGCTTCAG AGCTCTGCCA 
TCCGTAGTGG AGGAGGGGGT GACCTCACTC TTTGAAGAAG AGGTGATGGT AACCCAACTG 
ATCCCTGGAG TGGAAGGAAT ACCTTCTGGG GAGGAAACAA CTGTGGAGAC AGAGCTTTCC 
TCTGAGCCTG AGAATCAGAC AGCCCAGGGA ACGGAGGTCT TCCCAACTGA CGTATCACTG 
CTCTCAGCGA GACCATCTGC TTTTCCTCCA GCTACTGTAA TACCAGAGGA AACAAGTACC 
AATGCTTCCA TCCCCGAAGT GTCCGGAGAG TTCCCTGAGT CTGGAGAGCA CCCAACCAGT 
GGCGAACCTT CAGCATCTGG AGCCCCAGAC ACGAGTGGAG AACCAACTTC CGTAGGTTTT 
GAACTGAGTG GAGAGCAATC CGGGATTGGA GAAAGTGGAT TACCATCTGT AGACCTGCAG 
AGCAGTGGCT TTGTACCTGG AGAAAGTGGC CTTCCCTCAG GGGATGTGAG CGGGTTGCCT 
TCTGGCATTG TTGATATCAG CGGCTTGCCT TCTGCAGAGG AAGAGGTAAC GGTGTCTGTT 
TCGAGGATAC CAGAAGTTAG TGGAATGCCA TCTGGAGCTG AAAGCAGTGG TCTGCATTCT 
GGATTTAGTG GAGAAATCTC TGGCACTGAG CTTATCAGTG GCCTGCCATC TGGAGAGGAA 
AGTGGGCTCG CCTCTGGTTT TCCCACCATC TCCCTCGTGG ATTCCACTTT GGTGGAGGTT 
GTAACGGCAG CACCGGGACG GCAAGAGGAG GGAAAAGGAT CAATTGGAGT CAGTGGTGAA 
GAAGAGCTGT CAGGGTTTCC GTCTGCAGAG TGGGACAGCA GTGGGGCCAG AGGGCTGCCC 
TCGGGAGCTG AAACCAGTGG GGAGCAATCC GGGGTGCCCG AGCTCAGTGG GGAGCATTCC 
GGGGTGCCTG GGCTCAGTGG AGAGCCTTTT GAGGTGCCTG AGCTCAGCGG GGAGCATTCC 
GGGGTGACTG AGCTCAGTGG GGAGCATTCT GGGCTTCCTG AGCTCAGTGG AGAGCCTTTT 
GGGGTGCCTG AGCTCAGCGG GTTTCCCTCC GGACTGGACA TCAGTGGGGA ACCATCTGGA 
GCACCCGAGG TGAGCGGCCC GGTGGACGTG AGCGGCCTTA CCTCTGGTGT TGATGGAAGT 
GGTGAGGTCT CAGGCGTTAC CTTTATAAGC ACCAGCCTGC AAGAAGTGAC AACCCAGTCA 
GTTGCAGAAG CAGAAGCAAA AGAAATTCTA GAGATCAGTG GACTGCCTTC AGGAGAGACA 
TCAGGCATGG TGTCTGGGAG CTTAGATGTC AGCGGTCAGC CTTCGGGACA CATAGACTTT 
GGTGGGAGCG CTTCTGGAGT GCTGGAGATG AGCGGATTTC CAAGCGGAGC GGTTGAGAGC 
AGTGGAGAAG CCTCTGGGGT TGAAGTCACC AGTGTCCTCG CGTCTGGAGA GGAAAGTGGG 
CTCACCTCAG GCTTTCCCAC CGTGTCTCTC GTGGATACCA CGTTGGTAGA AGTTGTAACG 
CAGACATCAG TTGCTCAAGA GGTGGGAGAA GGACCATCTG GGATGATAGA AATCAGTGGA 
TTTCTTTCTG GAGACAGAGG AGTATCTGGA GAAGGGTCTG GAGCTGTGCA GTCTAGTGGG 
CTTCCTTCAG GAACAGGAGA CTTCAGCGGA GAGCCATCCG GGATCCCGTA TTTCAGCGGA 
GACATTTCTG GAGCCACAGA TCTAAGCGGA CAACCTTCCG CAGTGACTGA TATTAGTGGG 
GAGGACTCGG GACTTCCAGA AGTCACTTTA GTCACATCCG ATTTAGTAGA AGTTGTGACA 
AGGCCAACAG TTTCACAGGA GCTGGGTGGG GAAACAGCTG TGACGTTTCC CTATGTCTTT 
GGGCCAAGTG GTGAGGGCTC TGCATCTGGA GACCTGAGTG GGGGAGCATC TGCAGAAGGT 
GGTATAGAAA CATCAGCAGC TTATGAAATC AGTGGTGAGA GCTCTGCCTT CCCTGAAACG 
AGTATAGAAA CATCCACAGA TCAAGAAATC AGTGGGGAAG CATCTGCATA CCCTGAGATT 
AGCGTAGAAA CATCCACGCA TCTGGAAACC AGTGGGGAAA CATCTGCATA CCCTGAGATT 
AGCACAGAGA CGTCCACCAT TCAGGAAGTT AGTGGGGAAA CGTCTGCCTT TCCTGAAATT 
AGCACAGAAA CATCCACAAT ACAAGAGATC AGTGGGGAAA CATCCGCATT TCCTGAAATT 
AGAATAGAAA CATCCACCTT TCAAGAAATC AGTGGGGAAA CGTCCGCATT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGCGGCGAAA CGTCTGCGTT TCCCGAGATT 
ACCATAGAAG CCTCCACAGT CCATGAAACC AGTGGAGAAA CATCTGCCTT TCCCGAAATT 
AGCATAGAAA CATCCACAGT CCATGAAATC AGTGGGGAAA GTTCTGCCTT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGGGGTGAAA CATCTGCGTT CCCTGAGATT 
ACCATAGAAG CCTCCACCAT TCAAGAGATC AGTGGAGAAA CATCTGCATT TCCTGAAATT 
AGCATAGCAA CTTCCACCGT CCGTGAAATC AGCGGCGAAA CGTCTGCCTT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGGGGTGAAA CATCTGCATT GCCTGAGATT 
ACCGTAGAGA CATCCACTGT CCATGAAACC AGTGGGGAAG CGTCTGCCTT TCCTGAAATT 
AGCATAGAAA CTTCCACAAG ACAAGAAGCC AGGGGTGAAG CATCCGCCTA CCCTGAGGTT 
AGCATAGAGG CATCCACAAC TCAAGAAGTA AGTGGGGAAA GTTCTGCCTT CCCTGAAATT 
AGCGTAGAGA CATCCACAAG TCAGGAAGCC CGCGGTGAAA CATCTGCCTT TCCTGAGATT 
GGCATAGAGA CATCCACAGC CCACGAAGGC AGTGGGGAAA CTCCTGGGCT GCCTGCTGTT 
AGCACTGACA CTGCTGCCAC GTCTCTGGCC AGTGGTGAGC CCTCCGGTGC TCCTGAGAAG 
GAAACTCCCG ACACAACATC ACATCTGATC ACGGGCGTTT CAGGGGAAAC CTCTGTCCCA 
GATGCTGTAA TCAGTACCAG TGCTCCAGAT GTTGAACTAG CGCAGGGACC CAGAAACACT 
GAAGAGACTC AGCTTGAAAT AGAGCCCTCC ACTCCTGCGG CATCTGGACA AGAGACAGAA 
ACAGCTGCTG TCCTCGACAA TCCCCATCTG CCAGCCACTG CTACTGCTGC CCTGCATCCA 
GCCTCCCAAG AAGCAGTAGA TGCACTGGGA CCCACGACAG AAGACACTGA TGAGTGCCAC 
TCAAGCCCCT GTCTGAATGG AGCTACCTGC GTTGATGGCA TCGACTCTTT CAAATGCTTA 
TGCCTTCCCA GCTACGGAGG GGACCTGTGT GAGATCGACC TGGCAAACTG TGAGGAAGGC 
TGGATCAAGT TCCAGGGCCA CTGCTACAGG CACTTTGAAG AGAGGGAGAC GTGGATGGAC 
GCAGAGTCCA GGTGCAGAGA ACATCAAGCC CACCTGAGCA GCATCATTAC TCCAGAGGAA 
CAAGAATTTG TGAACAGCCA TGCACAAGAC TATCAGTGGA TCGGCCTCAG TGACAGAGCC 
GTGGAGAATG ATTTCCGCTG GTCTGATGGA CACTCGCTGC AATTTGAGAA CTGGCGGCCC 
AACCAACCTG ACAACTTCTT CTCCGCGGGC GAGGACTGCG TTGTGATGAT CTGGCATGAG 
CAAGGCGAAT GGAACGACGT CCCCTGCAAC TATCACCTGC CTTTCACGTG CAAGAAGGGA 
ACAGTTGCCT GTGGGGACCC ACCTGTAGTG GAGAACGCGC GGACCTTTGG CCGGAAGAAG 
GACCGCTATG AGATCAACTC CCTGGTGCGG TACCAGTGTG ACCACGGCTA CATCCAGCGC 
CACGTGCCCA CCATCCGCTG CCAACCCAAC GGGCACTGGG AGGAACCGCG GATATCCTGC 
ACAAACCCCT CCAGCTACCA ACGCCGGCTA TACAAGAGGA GCCCCCGGAG CCGGTTGAGA 
CCCGGTGTCG TGCACAGACC CACCCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACAN ( XM_015291978.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015147464.1 Gallus gallus aggrecan (ACAN), transcript variant X2, mRNA.

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CloneID	OGa28230
Clone ID Related Accession (Same CDS sequence)	XM_015291977.1 , XM_015291977.2 , XM_015291978.2 , NM_204955.2 , XM_015291978.1
Accession Version	XM_015291978.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	aggrecan core protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455924.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACTC TACTACTAGT GTTTGTGTGT TTGCAAGCCA TCACCACAGC TGCCTCCGCA 
GAGCTCTCAG ACAGTAGCGA TGGCCTGGAA GTGAAGATAC CTGAGCAGTC TCCCCTGCGT 
GTTGTCCTGG GAAGCTCCCT GAACATCCCC TGCTATTTCA ACATCCCAGA GGAAGAGGAC 
ACCAATGCTC TGCTGACCCC CCGGATCAAA TGGAGCAAGC TTTCCAATGG AACAGAGATT 
GTCTTACTAG TGGCCACCGG TGGGAAGATC CGGCTCAATG CAGAGTACAG AGAGGTGATC 
TCCTTGCCCA ATTACCCTGC CATCCCCACT GATGCCACCT TGGAAATCAA GGCGCTGAGA 
TCCAACCACA CTGGGATTTA TCGCTGTGAA GTGATGTATG GGATTGAGGA CAGACAAGAC 
ACCATAGAGG TCCTGGTGAA AGGCGTCGTG TTCCACTACA GAGCAATCTC CACAAGGTAC 
ACTTTGAACT TCGAGAGGGC AAAGCAGGCC TGTATCCAGA ACAGTGCTGT CATTGCCACC 
CCTGAGCAGC TGCAGGCTGC CTACGAGGAT GGGTACGAGC AGTGCGATGC CGGCTGGCTG 
GCTGATCAGA CTGTCAGGTA CCCCATCCAT CTGCCCCGGG AGCGCTGCTA CGGTGACAAG 
GATGAGTTTC CAGGAGTGAG AACCTACGGT GTCCGTGAGA CAGATGAAAC CTATGATGTT 
TACTGCTATG CAGAGCAAAT GCAAGGCAAA GTCTTCTACG CCACCTCCCC CGAGAAGTTC 
ACCTTCCAGG AAGCTTTTGA CAAATGCCAC AGCTTGGGAG CCCGCCTGGC CACCACGGGC 
GAGCTGTACT TGGCCTGGAA GGATGGCATG GACATGTGCA GTGCGGGCTG GCTGGCTGAC 
CGCAGCGTTC GCTACCCCAT TTCCAGAGCA CGGCCCAATT GTGGAGGGAA CCTGGTGGGC 
GTGCGGACCG TTTACCTGAA CCCTGCCAAC CAGACAGGGT ACCCTCACCC CAGCTCACGC 
TACGATGCCA TCTGTTACAG TGGTGATGAC TTCGAGGCTC TGGTCCCAGG GCTGTTCACT 
GACGAGGTTG GGACTGAGCT GGGCAGTGCT TTTACCATAC AGACCGTCAC ACAGACTGAG 
GTGGAGCTGC CCCTGCCACG CAATGTCACA GAAGAGGAGG CCCGTGGCAG CATCGCTACC 
CTGGAGCCCA TGGAGATCAC AGCCACCGCC ACTGAGCTGT ATGAGGCCTT CACCGTCCTG 
CCTGACCTCT TTGCCACCAG TGTCACAGTA GAGACAGCTT CCCCAAGGGA AGAGAACGTG 
ACCAGAGAGG AGATCACAGG GATATGGGCT GTGCCTGAAG AGGTCACTAC ATCGGTCTCA 
GGCACTGCTT TCACCACCGG AATGGCAGAG GTGAGCTCAG TGGAAGAGGC CATAGCAGTG 
ACTGCCACAC CAGGACTGGA GTCTGCCTCT CCATTCACCA TAGAAGATCA TCTCGTGCAA 
GTGACAGCAG CCCCTGATGT TGCCCTCCTC CCCAGGCAGC CCATTTCCCC CACTGGTGTG 
GTGTTCCACT ACCGTGCTGC CACCAGCAGA TACGCCTTCT CCTTCATCCA AGCCCAGCAG 
GCCTGCCTGG AGAACAATGC CGTTATTGCC ACTCCCGAGC AGCTCCAGGC TGCCTACGAG 
GCTGGCTTTG ATCAGTGCGA TGCCGGCTGG CTGCGGGACC AGACGGTCAG GTATCCCATT 
GTGAATCCCC GCAGCAACTG TGTAGGAGAC AAAGAGAGCT CCCCAGGTGT GCGGTCATAC 
GGCATGCGCC CGGCCTCAGA GACCTACGAT GTGTACTGTT ACATCGACAG GCTAAAGGGT 
GAGGTGTTTT TTGCCACCCA GCCAGAGCAG TTCACCTTCC AAGAAGCCCA GCTGTACTGT 
GAAAGCCAAA ATGCCACGCT GGCCTCTGCT GGGCAACTCC ACGCTGCCTG GAAGCAGGGC 
CTGGATAGGT GCTACCCTGG GTGGCTGGCC GACGGCAGCT TGCGGTACCC CATTGTGAGC 
CCCCGACCTG CCTGCGGGGG GGATGCACCT GGTGTGAGGA CCATCTACCA GCACCACAAC 
CAGACGGGCT TTCCTGACCC TCTGTCACGG CATCACGCTT TCTGCTTCAG AGCTCTGCCA 
TCCGTAGTGG AGGAGGGGGT GACCTCACTC TTTGAAGAAG AGGTGATGGT AACCCAACTG 
ATCCCTGGAG TGGAAGGAAT ACCTTCTGGG GAGGAAACAA CTGTGGAGAC AGAGCTTTCC 
TCTGAGCCTG AGAATCAGAC AGCCCAGGGA ACGGAGGTCT TCCCAACTGA CGTATCACTG 
CTCTCAGCGA GACCATCTGC TTTTCCTCCA GCTACTGTAA TACCAGAGGA AACAAGTACC 
AATGCTTCCA TCCCCGAAGT GTCCGGAGAG TTCCCTGAGT CTGGAGAGCA CCCAACCAGT 
GGCGAACCTT CAGCATCTGG AGCCCCAGAC ACGAGTGGAG AACCAACTTC CGTAGGTTTT 
GAACTGAGTG GAGAGCAATC CGGGATTGGA GAAAGTGGAT TACCATCTGT AGACCTGCAG 
AGCAGTGGCT TTGTACCTGG AGAAAGTGGC CTTCCCTCAG GGGATGTGAG CGGGTTGCCT 
TCTGGCATTG TTGATATCAG CGGCTTGCCT TCTGCAGAGG AAGAGGTAAC GGTGTCTGTT 
TCGAGGATAC CAGAAGTTAG TGGAATGCCA TCTGGAGCTG AAAGCAGTGG TCTGCATTCT 
GGATTTAGTG GAGAAATCTC TGGCACTGAG CTTATCAGTG GCCTGCCATC TGGAGAGGAA 
AGTGGGCTCG CCTCTGGTTT TCCCACCATC TCCCTCGTGG ATTCCACTTT GGTGGAGGTT 
GTAACGGCAG CACCGGGACG GCAAGAGGAG GGAAAAGGAT CAATTGGAGT CAGTGGTGAA 
GAAGAGCTGT CAGGGTTTCC GTCTGCAGAG TGGGACAGCA GTGGGGCCAG AGGGCTGCCC 
TCGGGAGCTG AAACCAGTGG GGAGCAATCC GGGGTGCCCG AGCTCAGTGG GGAGCATTCC 
GGGGTGCCTG GGCTCAGTGG AGAGCCTTTT GAGGTGCCTG AGCTCAGCGG GGAGCATTCC 
GGGGTGACTG AGCTCAGTGG GGAGCATTCT GGGCTTCCTG AGCTCAGTGG AGAGCCTTTT 
GGGGTGCCTG AGCTCAGCGG GTTTCCCTCC GGACTGGACA TCAGTGGGGA ACCATCTGGA 
GCACCCGAGG TGAGCGGCCC GGTGGACGTG AGCGGCCTTA CCTCTGGTGT TGATGGAAGT 
GGTGAGGTCT CAGGCGTTAC CTTTATAAGC ACCAGCCTGC AAGAAGTGAC AACCCAGTCA 
GTTGCAGAAG CAGAAGCAAA AGAAATTCTA GAGATCAGTG GACTGCCTTC AGGAGAGACA 
TCAGGCATGG TGTCTGGGAG CTTAGATGTC AGCGGTCAGC CTTCGGGACA CATAGACTTT 
GGTGGGAGCG CTTCTGGAGT GCTGGAGATG AGCGGATTTC CAAGCGGAGC GGTTGAGAGC 
AGTGGAGAAG CCTCTGGGGT TGAAGTCACC AGTGTCCTCG CGTCTGGAGA GGAAAGTGGG 
CTCACCTCAG GCTTTCCCAC CGTGTCTCTC GTGGATACCA CGTTGGTAGA AGTTGTAACG 
CAGACATCAG TTGCTCAAGA GGTGGGAGAA GGACCATCTG GGATGATAGA AATCAGTGGA 
TTTCTTTCTG GAGACAGAGG AGTATCTGGA GAAGGGTCTG GAGCTGTGCA GTCTAGTGGG 
CTTCCTTCAG GAACAGGAGA CTTCAGCGGA GAGCCATCCG GGATCCCGTA TTTCAGCGGA 
GACATTTCTG GAGCCACAGA TCTAAGCGGA CAACCTTCCG CAGTGACTGA TATTAGTGGG 
GAGGACTCGG GACTTCCAGA AGTCACTTTA GTCACATCCG ATTTAGTAGA AGTTGTGACA 
AGGCCAACAG TTTCACAGGA GCTGGGTGGG GAAACAGCTG TGACGTTTCC CTATGTCTTT 
GGGCCAAGTG GTGAGGGCTC TGCATCTGGA GACCTGAGTG GGGGAGCATC TGCAGAAGGT 
GGTATAGAAA CATCAGCAGC TTATGAAATC AGTGGTGAGA GCTCTGCCTT CCCTGAAACG 
AGTATAGAAA CATCCACAGA TCAAGAAATC AGTGGGGAAG CATCTGCATA CCCTGAGATT 
AGCGTAGAAA CATCCACGCA TCTGGAAACC AGTGGGGAAA CATCTGCATA CCCTGAGATT 
AGCACAGAGA CGTCCACCAT TCAGGAAGTT AGTGGGGAAA CGTCTGCCTT TCCTGAAATT 
AGCACAGAAA CATCCACAAT ACAAGAGATC AGTGGGGAAA CATCCGCATT TCCTGAAATT 
AGAATAGAAA CATCCACCTT TCAAGAAATC AGTGGGGAAA CGTCCGCATT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGCGGCGAAA CGTCTGCGTT TCCCGAGATT 
ACCATAGAAG CCTCCACAGT CCATGAAACC AGTGGAGAAA CATCTGCCTT TCCCGAAATT 
AGCATAGAAA CATCCACAGT CCATGAAATC AGTGGGGAAA GTTCTGCCTT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGGGGTGAAA CATCTGCGTT CCCTGAGATT 
ACCATAGAAG CCTCCACCAT TCAAGAGATC AGTGGAGAAA CATCTGCATT TCCTGAAATT 
AGCATAGCAA CTTCCACCGT CCGTGAAATC AGCGGCGAAA CGTCTGCCTT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGGGGTGAAA CATCTGCATT GCCTGAGATT 
ACCGTAGAGA CATCCACTGT CCATGAAACC AGTGGGGAAG CGTCTGCCTT TCCTGAAATT 
AGCATAGAAA CTTCCACAAG ACAAGAAGCC AGGGGTGAAG CATCCGCCTA CCCTGAGGTT 
AGCATAGAGG CATCCACAAC TCAAGAAGTA AGTGGGGAAA GTTCTGCCTT CCCTGAAATT 
AGCGTAGAGA CATCCACAAG TCAGGAAGCC CGCGGTGAAA CATCTGCCTT TCCTGAGATT 
GGCATAGAGA CATCCACAGC CCACGAAGGC AGTGGGGAAA CTCCTGGGCT GCCTGCTGTT 
AGCACTGACA CTGCTGCCAC GTCTCTGGCC AGTGGTGAGC CCTCCGGTGC TCCTGAGAAG 
GAAACTCCCG ACACAACATC ACATCTGATC ACGGGCGTTT CAGGGGAAAC CTCTGTCCCA 
GATGCTGTAA TCAGTACCAG TGCTCCAGAT GTTGAACTAG CGCAGGGACC CAGAAACACT 
GAAGAGACTC AGCTTGAAAT AGAGCCCTCC ACTCCTGCGG CATCTGGACA AGAGACAGAA 
ACAGCTGCTG TCCTCGACAA TCCCCATCTG CCAGCCACTG CTACTGCTGC CCTGCATCCA 
GCCTCCCAAG AAGCAGTAGA TGCACTGGGA CCCACGACAG AAGACACTGA TGAGTGCCAC 
TCAAGCCCCT GTCTGAATGG AGCTACCTGC GTTGATGGCA TCGACTCTTT CAAATGCTTA 
TGCCTTCCCA GCTACGGAGG GGACCTGTGT GAGATCGACC TGGCAAACTG TGAGGAAGGC 
TGGATCAAGT TCCAGGGCCA CTGCTACAGG CACTTTGAAG AGAGGGAGAC GTGGATGGAC 
GCAGAGTCCA GGTGCAGAGA ACATCAAGCC CACCTGAGCA GCATCATTAC TCCAGAGGAA 
CAAGAATTTG TGAACAGCCA TGCACAAGAC TATCAGTGGA TCGGCCTCAG TGACAGAGCC 
GTGGAGAATG ATTTCCGCTG GTCTGATGGA CACTCGCTGC AATTTGAGAA CTGGCGGCCC 
AACCAACCTG ACAACTTCTT CTCCGCGGGC GAGGACTGCG TTGTGATGAT CTGGCATGAG 
CAAGGCGAAT GGAACGACGT CCCCTGCAAC TATCACCTGC CTTTCACGTG CAAGAAGGGA 
ACAGTTGCCT GTGGGGACCC ACCTGTAGTG GAGAACGCGC GGACCTTTGG CCGGAAGAAG 
GACCGCTATG AGATCAACTC CCTGGTGCGG TACCAGTGTG ACCACGGCTA CATCCAGCGC 
CACGTGCCCA CCATCCGCTG CCAACCCAAC GGGCACTGGG AGGAACCGCG GATATCCTGC 
ACAAACCCCT CCAGCTACCA ACGCCGGCTA TACAAGAGGA GCCCCCGGAG CCGGTTGAGA 
CCCGGTGTCG TGCACAGACC CACCCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015147464.1
CDS	1484..7753
Translation	MTTLLLVFVCLQAITTAASAELSDSSDGLEVKIPEQSPLRVVLGSSLNIPCYFNIPEEEDTNALLTPRIKWSKLSNGTEIVLLVATGGKIRLNAEYREVISLPNYPAIPTDATLEIKALRSNHTGIYRCEVMYGIEDRQDTIEVLVKGVVFHYRAISTRYTLNFERAKQACIQNSAVIATPEQLQAAYEDGYEQCDAGWLADQTVRYPIHLPRERCYGDKDEFPGVRTYGVRETDETYDVYCYAEQMQGKVFYATSPEKFTFQEAFDKCHSLGARLATTGELYLAWKDGMDMCSAGWLADRSVRYPISRARPNCGGNLVGVRTVYLNPANQTGYPHPSSRYDAICYSGDDFEALVPGLFTDEVGTELGSAFTIQTVTQTEVELPLPRNVTEEEARGSIATLEPMEITATATELYEAFTVLPDLFATSVTVETASPREENVTREEITGIWAVPEEVTTSVSGTAFTTGMAEVSSVEEAIAVTATPGLESASPFTIEDHLVQVTAAPDVALLPRQPISPTGVVFHYRAATSRYAFSFIQAQQACLENNAVIATPEQLQAAYEAGFDQCDAGWLRDQTVRYPIVNPRSNCVGDKESSPGVRSYGMRPASETYDVYCYIDRLKGEVFFATQPEQFTFQEAQLYCESQNATLASAGQLHAAWKQGLDRCYPGWLADGSLRYPIVSPRPACGGDAPGVRTIYQHHNQTGFPDPLSRHHAFCFRALPSVVEEGVTSLFEEEVMVTQLIPGVEGIPSGEETTVETELSSEPENQTAQGTEVFPTDVSLLSARPSAFPPATVIPEETSTNASIPEVSGEFPESGEHPTSGEPSASGAPDTSGEPTSVGFELSGEQSGIGESGLPSVDLQSSGFVPGESGLPSGDVSGLPSGIVDISGLPSAEEEVTVSVSRIPEVSGMPSGAESSGLHSGFSGEISGTELISGLPSGEESGLASGFPTISLVDSTLVEVVTAAPGRQEEGKGSIGVSGEEELSGFPSAEWDSSGARGLPSGAETSGEQSGVPELSGEHSGVPGLSGEPFEVPELSGEHSGVTELSGEHSGLPELSGEPFGVPELSGFPSGLDISGEPSGAPEVSGPVDVSGLTSGVDGSGEVSGVTFISTSLQEVTTQSVAEAEAKEILEISGLPSGETSGMVSGSLDVSGQPSGHIDFGGSASGVLEMSGFPSGAVESSGEASGVEVTSVLASGEESGLTSGFPTVSLVDTTLVEVVTQTSVAQEVGEGPSGMIEISGFLSGDRGVSGEGSGAVQSSGLPSGTGDFSGEPSGIPYFSGDISGATDLSGQPSAVTDISGEDSGLPEVTLVTSDLVEVVTRPTVSQELGGETAVTFPYVFGPSGEGSASGDLSGGASAEGGIETSAAYEISGESSAFPETSIETSTDQEISGEASAYPEISVETSTHLETSGETSAYPEISTETSTIQEVSGETSAFPEISTETSTIQEISGETSAFPEIRIETSTFQEISGETSAFPEIRIETSTSQEARGETSAFPEITIEASTVHETSGETSAFPEISIETSTVHEISGESSAFPEIRIETSTSQEARGETSAFPEITIEASTIQEISGETSAFPEISIATSTVREISGETSAFPEIRIETSTSQEARGETSALPEITVETSTVHETSGEASAFPEISIETSTRQEARGEASAYPEVSIEASTTQEVSGESSAFPEISVETSTSQEARGETSAFPEIGIETSTAHEGSGETPGLPAVSTDTAATSLASGEPSGAPEKETPDTTSHLITGVSGETSVPDAVISTSAPDVELAQGPRNTEETQLEIEPSTPAASGQETETAAVLDNPHLPATATAALHPASQEAVDALGPTTEDTDECHSSPCLNGATCVDGIDSFKCLCLPSYGGDLCEIDLANCEEGWIKFQGHCYRHFEERETWMDAESRCREHQAHLSSIITPEEQEFVNSHAQDYQWIGLSDRAVENDFRWSDGHSLQFENWRPNQPDNFFSAGEDCVVMIWHEQGEWNDVPCNYHLPFTCKKGTVACGDPPVVENARTFGRKKDRYEINSLVRYQCDHGYIQRHVPTIRCQPNGHWEEPRISCTNPSSYQRRLYKRSPRSRLRPGVVHRPTH

Target ORF information:

RefSeq Version	XM_015291978.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus aggrecan (ACAN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291978.1

ORF Insert Sequence:

ATGACCACTC TACTACTAGT GTTTGTGTGT TTGCAAGCCA TCACCACAGC TGCCTCCGCA 
GAGCTCTCAG ACAGTAGCGA TGGCCTGGAA GTGAAGATAC CTGAGCAGTC TCCCCTGCGT 
GTTGTCCTGG GAAGCTCCCT GAACATCCCC TGCTATTTCA ACATCCCAGA GGAAGAGGAC 
ACCAATGCTC TGCTGACCCC CCGGATCAAA TGGAGCAAGC TTTCCAATGG AACAGAGATT 
GTCTTACTAG TGGCCACCGG TGGGAAGATC CGGCTCAATG CAGAGTACAG AGAGGTGATC 
TCCTTGCCCA ATTACCCTGC CATCCCCACT GATGCCACCT TGGAAATCAA GGCGCTGAGA 
TCCAACCACA CTGGGATTTA TCGCTGTGAA GTGATGTATG GGATTGAGGA CAGACAAGAC 
ACCATAGAGG TCCTGGTGAA AGGCGTCGTG TTCCACTACA GAGCAATCTC CACAAGGTAC 
ACTTTGAACT TCGAGAGGGC AAAGCAGGCC TGTATCCAGA ACAGTGCTGT CATTGCCACC 
CCTGAGCAGC TGCAGGCTGC CTACGAGGAT GGGTACGAGC AGTGCGATGC CGGCTGGCTG 
GCTGATCAGA CTGTCAGGTA CCCCATCCAT CTGCCCCGGG AGCGCTGCTA CGGTGACAAG 
GATGAGTTTC CAGGAGTGAG AACCTACGGT GTCCGTGAGA CAGATGAAAC CTATGATGTT 
TACTGCTATG CAGAGCAAAT GCAAGGCAAA GTCTTCTACG CCACCTCCCC CGAGAAGTTC 
ACCTTCCAGG AAGCTTTTGA CAAATGCCAC AGCTTGGGAG CCCGCCTGGC CACCACGGGC 
GAGCTGTACT TGGCCTGGAA GGATGGCATG GACATGTGCA GTGCGGGCTG GCTGGCTGAC 
CGCAGCGTTC GCTACCCCAT TTCCAGAGCA CGGCCCAATT GTGGAGGGAA CCTGGTGGGC 
GTGCGGACCG TTTACCTGAA CCCTGCCAAC CAGACAGGGT ACCCTCACCC CAGCTCACGC 
TACGATGCCA TCTGTTACAG TGGTGATGAC TTCGAGGCTC TGGTCCCAGG GCTGTTCACT 
GACGAGGTTG GGACTGAGCT GGGCAGTGCT TTTACCATAC AGACCGTCAC ACAGACTGAG 
GTGGAGCTGC CCCTGCCACG CAATGTCACA GAAGAGGAGG CCCGTGGCAG CATCGCTACC 
CTGGAGCCCA TGGAGATCAC AGCCACCGCC ACTGAGCTGT ATGAGGCCTT CACCGTCCTG 
CCTGACCTCT TTGCCACCAG TGTCACAGTA GAGACAGCTT CCCCAAGGGA AGAGAACGTG 
ACCAGAGAGG AGATCACAGG GATATGGGCT GTGCCTGAAG AGGTCACTAC ATCGGTCTCA 
GGCACTGCTT TCACCACCGG AATGGCAGAG GTGAGCTCAG TGGAAGAGGC CATAGCAGTG 
ACTGCCACAC CAGGACTGGA GTCTGCCTCT CCATTCACCA TAGAAGATCA TCTCGTGCAA 
GTGACAGCAG CCCCTGATGT TGCCCTCCTC CCCAGGCAGC CCATTTCCCC CACTGGTGTG 
GTGTTCCACT ACCGTGCTGC CACCAGCAGA TACGCCTTCT CCTTCATCCA AGCCCAGCAG 
GCCTGCCTGG AGAACAATGC CGTTATTGCC ACTCCCGAGC AGCTCCAGGC TGCCTACGAG 
GCTGGCTTTG ATCAGTGCGA TGCCGGCTGG CTGCGGGACC AGACGGTCAG GTATCCCATT 
GTGAATCCCC GCAGCAACTG TGTAGGAGAC AAAGAGAGCT CCCCAGGTGT GCGGTCATAC 
GGCATGCGCC CGGCCTCAGA GACCTACGAT GTGTACTGTT ACATCGACAG GCTAAAGGGT 
GAGGTGTTTT TTGCCACCCA GCCAGAGCAG TTCACCTTCC AAGAAGCCCA GCTGTACTGT 
GAAAGCCAAA ATGCCACGCT GGCCTCTGCT GGGCAACTCC ACGCTGCCTG GAAGCAGGGC 
CTGGATAGGT GCTACCCTGG GTGGCTGGCC GACGGCAGCT TGCGGTACCC CATTGTGAGC 
CCCCGACCTG CCTGCGGGGG GGATGCACCT GGTGTGAGGA CCATCTACCA GCACCACAAC 
CAGACGGGCT TTCCTGACCC TCTGTCACGG CATCACGCTT TCTGCTTCAG AGCTCTGCCA 
TCCGTAGTGG AGGAGGGGGT GACCTCACTC TTTGAAGAAG AGGTGATGGT AACCCAACTG 
ATCCCTGGAG TGGAAGGAAT ACCTTCTGGG GAGGAAACAA CTGTGGAGAC AGAGCTTTCC 
TCTGAGCCTG AGAATCAGAC AGCCCAGGGA ACGGAGGTCT TCCCAACTGA CGTATCACTG 
CTCTCAGCGA GACCATCTGC TTTTCCTCCA GCTACTGTAA TACCAGAGGA AACAAGTACC 
AATGCTTCCA TCCCCGAAGT GTCCGGAGAG TTCCCTGAGT CTGGAGAGCA CCCAACCAGT 
GGCGAACCTT CAGCATCTGG AGCCCCAGAC ACGAGTGGAG AACCAACTTC CGTAGGTTTT 
GAACTGAGTG GAGAGCAATC CGGGATTGGA GAAAGTGGAT TACCATCTGT AGACCTGCAG 
AGCAGTGGCT TTGTACCTGG AGAAAGTGGC CTTCCCTCAG GGGATGTGAG CGGGTTGCCT 
TCTGGCATTG TTGATATCAG CGGCTTGCCT TCTGCAGAGG AAGAGGTAAC GGTGTCTGTT 
TCGAGGATAC CAGAAGTTAG TGGAATGCCA TCTGGAGCTG AAAGCAGTGG TCTGCATTCT 
GGATTTAGTG GAGAAATCTC TGGCACTGAG CTTATCAGTG GCCTGCCATC TGGAGAGGAA 
AGTGGGCTCG CCTCTGGTTT TCCCACCATC TCCCTCGTGG ATTCCACTTT GGTGGAGGTT 
GTAACGGCAG CACCGGGACG GCAAGAGGAG GGAAAAGGAT CAATTGGAGT CAGTGGTGAA 
GAAGAGCTGT CAGGGTTTCC GTCTGCAGAG TGGGACAGCA GTGGGGCCAG AGGGCTGCCC 
TCGGGAGCTG AAACCAGTGG GGAGCAATCC GGGGTGCCCG AGCTCAGTGG GGAGCATTCC 
GGGGTGCCTG GGCTCAGTGG AGAGCCTTTT GAGGTGCCTG AGCTCAGCGG GGAGCATTCC 
GGGGTGACTG AGCTCAGTGG GGAGCATTCT GGGCTTCCTG AGCTCAGTGG AGAGCCTTTT 
GGGGTGCCTG AGCTCAGCGG GTTTCCCTCC GGACTGGACA TCAGTGGGGA ACCATCTGGA 
GCACCCGAGG TGAGCGGCCC GGTGGACGTG AGCGGCCTTA CCTCTGGTGT TGATGGAAGT 
GGTGAGGTCT CAGGCGTTAC CTTTATAAGC ACCAGCCTGC AAGAAGTGAC AACCCAGTCA 
GTTGCAGAAG CAGAAGCAAA AGAAATTCTA GAGATCAGTG GACTGCCTTC AGGAGAGACA 
TCAGGCATGG TGTCTGGGAG CTTAGATGTC AGCGGTCAGC CTTCGGGACA CATAGACTTT 
GGTGGGAGCG CTTCTGGAGT GCTGGAGATG AGCGGATTTC CAAGCGGAGC GGTTGAGAGC 
AGTGGAGAAG CCTCTGGGGT TGAAGTCACC AGTGTCCTCG CGTCTGGAGA GGAAAGTGGG 
CTCACCTCAG GCTTTCCCAC CGTGTCTCTC GTGGATACCA CGTTGGTAGA AGTTGTAACG 
CAGACATCAG TTGCTCAAGA GGTGGGAGAA GGACCATCTG GGATGATAGA AATCAGTGGA 
TTTCTTTCTG GAGACAGAGG AGTATCTGGA GAAGGGTCTG GAGCTGTGCA GTCTAGTGGG 
CTTCCTTCAG GAACAGGAGA CTTCAGCGGA GAGCCATCCG GGATCCCGTA TTTCAGCGGA 
GACATTTCTG GAGCCACAGA TCTAAGCGGA CAACCTTCCG CAGTGACTGA TATTAGTGGG 
GAGGACTCGG GACTTCCAGA AGTCACTTTA GTCACATCCG ATTTAGTAGA AGTTGTGACA 
AGGCCAACAG TTTCACAGGA GCTGGGTGGG GAAACAGCTG TGACGTTTCC CTATGTCTTT 
GGGCCAAGTG GTGAGGGCTC TGCATCTGGA GACCTGAGTG GGGGAGCATC TGCAGAAGGT 
GGTATAGAAA CATCAGCAGC TTATGAAATC AGTGGTGAGA GCTCTGCCTT CCCTGAAACG 
AGTATAGAAA CATCCACAGA TCAAGAAATC AGTGGGGAAG CATCTGCATA CCCTGAGATT 
AGCGTAGAAA CATCCACGCA TCTGGAAACC AGTGGGGAAA CATCTGCATA CCCTGAGATT 
AGCACAGAGA CGTCCACCAT TCAGGAAGTT AGTGGGGAAA CGTCTGCCTT TCCTGAAATT 
AGCACAGAAA CATCCACAAT ACAAGAGATC AGTGGGGAAA CATCCGCATT TCCTGAAATT 
AGAATAGAAA CATCCACCTT TCAAGAAATC AGTGGGGAAA CGTCCGCATT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGCGGCGAAA CGTCTGCGTT TCCCGAGATT 
ACCATAGAAG CCTCCACAGT CCATGAAACC AGTGGAGAAA CATCTGCCTT TCCCGAAATT 
AGCATAGAAA CATCCACAGT CCATGAAATC AGTGGGGAAA GTTCTGCCTT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGGGGTGAAA CATCTGCGTT CCCTGAGATT 
ACCATAGAAG CCTCCACCAT TCAAGAGATC AGTGGAGAAA CATCTGCATT TCCTGAAATT 
AGCATAGCAA CTTCCACCGT CCGTGAAATC AGCGGCGAAA CGTCTGCCTT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGGGGTGAAA CATCTGCATT GCCTGAGATT 
ACCGTAGAGA CATCCACTGT CCATGAAACC AGTGGGGAAG CGTCTGCCTT TCCTGAAATT 
AGCATAGAAA CTTCCACAAG ACAAGAAGCC AGGGGTGAAG CATCCGCCTA CCCTGAGGTT 
AGCATAGAGG CATCCACAAC TCAAGAAGTA AGTGGGGAAA GTTCTGCCTT CCCTGAAATT 
AGCGTAGAGA CATCCACAAG TCAGGAAGCC CGCGGTGAAA CATCTGCCTT TCCTGAGATT 
GGCATAGAGA CATCCACAGC CCACGAAGGC AGTGGGGAAA CTCCTGGGCT GCCTGCTGTT 
AGCACTGACA CTGCTGCCAC GTCTCTGGCC AGTGGTGAGC CCTCCGGTGC TCCTGAGAAG 
GAAACTCCCG ACACAACATC ACATCTGATC ACGGGCGTTT CAGGGGAAAC CTCTGTCCCA 
GATGCTGTAA TCAGTACCAG TGCTCCAGAT GTTGAACTAG CGCAGGGACC CAGAAACACT 
GAAGAGACTC AGCTTGAAAT AGAGCCCTCC ACTCCTGCGG CATCTGGACA AGAGACAGAA 
ACAGCTGCTG TCCTCGACAA TCCCCATCTG CCAGCCACTG CTACTGCTGC CCTGCATCCA 
GCCTCCCAAG AAGCAGTAGA TGCACTGGGA CCCACGACAG AAGACACTGA TGAGTGCCAC 
TCAAGCCCCT GTCTGAATGG AGCTACCTGC GTTGATGGCA TCGACTCTTT CAAATGCTTA 
TGCCTTCCCA GCTACGGAGG GGACCTGTGT GAGATCGACC TGGCAAACTG TGAGGAAGGC 
TGGATCAAGT TCCAGGGCCA CTGCTACAGG CACTTTGAAG AGAGGGAGAC GTGGATGGAC 
GCAGAGTCCA GGTGCAGAGA ACATCAAGCC CACCTGAGCA GCATCATTAC TCCAGAGGAA 
CAAGAATTTG TGAACAGCCA TGCACAAGAC TATCAGTGGA TCGGCCTCAG TGACAGAGCC 
GTGGAGAATG ATTTCCGCTG GTCTGATGGA CACTCGCTGC AATTTGAGAA CTGGCGGCCC 
AACCAACCTG ACAACTTCTT CTCCGCGGGC GAGGACTGCG TTGTGATGAT CTGGCATGAG 
CAAGGCGAAT GGAACGACGT CCCCTGCAAC TATCACCTGC CTTTCACGTG CAAGAAGGGA 
ACAGTTGCCT GTGGGGACCC ACCTGTAGTG GAGAACGCGC GGACCTTTGG CCGGAAGAAG 
GACCGCTATG AGATCAACTC CCTGGTGCGG TACCAGTGTG ACCACGGCTA CATCCAGCGC 
CACGTGCCCA CCATCCGCTG CCAACCCAAC GGGCACTGGG AGGAACCGCG GATATCCTGC 
ACAAACCCCT CCAGCTACCA ACGCCGGCTA TACAAGAGGA GCCCCCGGAG CCGGTTGAGA 
CCCGGTGTCG TGCACAGACC CACCCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACAN ( XM_015291979.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015147465.1 Gallus gallus aggrecan (ACAN), transcript variant X3, mRNA.

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CloneID	OGa40533
Clone ID Related Accession (Same CDS sequence)	XM_015291979.2 , XM_015291979.1
Accession Version	XM_015291979.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6156bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	aggrecan core protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006097.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291979.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACTC TACTACTAGT GTTTGTGTGT TTGCAAGCCA TCACCACAGC TGCCTCCGCA 
GAGCTCTCAG ACAGTAGCGA TGGCCTGGAA GTGAAGATAC CTGAGCAGTC TCCCCTGCGT 
GTTGTCCTGG GAAGCTCCCT GAACATCCCC TGCTATTTCA ACATCCCAGA GGAAGAGGAC 
ACCAATGCTC TGCTGACCCC CCGGATCAAA TGGAGCAAGC TTTCCAATGG AACAGAGATT 
GTCTTACTAG TGGCCACCGG TGGGAAGATC CGGCTCAATG CAGAGTACAG AGAGGTGATC 
TCCTTGCCCA ATTACCCTGC CATCCCCACT GATGCCACCT TGGAAATCAA GGCGCTGAGA 
TCCAACCACA CTGGGATTTA TCGCTGTGAA GTGATGTATG GGATTGAGGA CAGACAAGAC 
ACCATAGAGG TCCTGGTGAA AGGCGTCGTG TTCCACTACA GAGCAATCTC CACAAGGTAC 
ACTTTGAACT TCGAGAGGGC AAAGCAGGCC TGTATCCAGA ACAGTGCTGT CATTGCCACC 
CCTGAGCAGC TGCAGGCTGC CTACGAGGAT GGGTACGAGC AGTGCGATGC CGGCTGGCTG 
GCTGATCAGA CTGTCAGGTA CCCCATCCAT CTGCCCCGGG AGCGCTGCTA CGGTGACAAG 
GATGAGTTTC CAGGAGTGAG AACCTACGGT GTCCGTGAGA CAGATGAAAC CTATGATGTT 
TACTGCTATG CAGAGCAAAT GCAAGGCAAA GTCTTCTACG CCACCTCCCC CGAGAAGTTC 
ACCTTCCAGG AAGCTTTTGA CAAATGCCAC AGCTTGGGAG CCCGCCTGGC CACCACGGGC 
GAGCTGTACT TGGCCTGGAA GGATGGCATG GACATGTGCA GTGCGGGCTG GCTGGCTGAC 
CGCAGCGTTC GCTACCCCAT TTCCAGAGCA CGGCCCAATT GTGGAGGGAA CCTGGTGGGC 
GTGCGGACCG TTTACCTGAA CCCTGCCAAC CAGACAGGGT ACCCTCACCC CAGCTCACGC 
TACGATGCCA TCTGTTACAG TGGTGATGAC TTCGAGGCTC TGGTCCCAGG GCTGTTCACT 
GACGAGGTTG GGACTGAGCT GGGCAGTGCT TTTACCATAC AGACCGTCAC ACAGACTGAG 
GTGGAGCTGC CCCTGCCACG CAATGTCACA GAAGAGGAGG CCCGTGGCAG CATCGCTACC 
CTGGAGCCCA TGGAGATCAC AGCCACCGCC ACTGAGCTGT ATGAGGCCTT CACCGTCCTG 
CCTGACCTCT TTGCCACCAG TGTCACAGTA GAGACAGCTT CCCCAAGGGA AGAGAACGTG 
ACCAGAGAGG AGATCACAGG GATATGGGCT GTGCCTGAAG AGGTCACTAC ATCGGTCTCA 
GGCACTGCTT TCACCACCGG AATGGCAGAG GTGAGCTCAG TGGAAGAGGC CATAGCAGTG 
ACTGCCACAC CAGGACTGGA GTCTGCCTCT CCATTCACCA TAGAAGATCA TCTCGTGCAA 
GTGACAGCAG CCCCTGATGT TGCCCTCCTC CCCAGGCAGC CCATTTCCCC CACTGGTGTG 
GTGTTCCACT ACCGTGCTGC CACCAGCAGA TACGCCTTCT CCTTCATCCA AGCCCAGCAG 
GCCTGCCTGG AGAACAATGC CGTTATTGCC ACTCCCGAGC AGCTCCAGGC TGCCTACGAG 
GCTGGCTTTG ATCAGTGCGA TGCCGGCTGG CTGCGGGACC AGACGGTCAG GTATCCCATT 
GTGAATCCCC GCAGCAACTG TGTAGGAGAC AAAGAGAGCT CCCCAGGTGT GCGGTCATAC 
GGCATGCGCC CGGCCTCAGA GACCTACGAT GTGTACTGTT ACATCGACAG GCTAAAGGGT 
GAGGTGTTTT TTGCCACCCA GCCAGAGCAG TTCACCTTCC AAGAAGCCCA GCTGTACTGT 
GAAAGCCAAA ATGCCACGCT GGCCTCTGCT GGGCAACTCC ACGCTGCCTG GAAGCAGGGC 
CTGGATAGGT GCTACCCTGG GTGGCTGGCC GACGGCAGCT TGCGGTACCC CATTGTGAGC 
CCCCGACCTG CCTGCGGGGG GGATGCACCT GGTGTGAGGA CCATCTACCA GCACCACAAC 
CAGACGGGCT TTCCTGACCC TCTGTCACGG CATCACGCTT TCTGCTTCAG AGCTCTGCCA 
TCCGTAGTGG AGGAGGGGGT GACCTCACTC TTTGAAGAAG AGGTGATGGT AACCCAACTG 
ATCCCTGGAG TGGAAGGAAT ACCTTCTGGG GAGGAAACAA CTGTGGAGAC AGAGCTTTCC 
TCTGAGCCTG AGAATCAGAC AGCCCAGGGA ACGGAGGTCT TCCCAACTGA CGTATCACTG 
CTCTCAGCGA GACCATCTGC TTTTCCTCCA GCTACTGTAA TACCAGAGGA AACAAGTACC 
AATGCTTCCA TCCCCGAAGT GTCCGGAGAG TTCCCTGAGT CTGGAGAGCA CCCAACCAGT 
GGCGAACCTT CAGCATCTGG AGCCCCAGAC ACGAGTGGAG AACCAACTTC CGTAGGTTTT 
GAACTGAGTG GAGAGCAATC CGGGATTGGA GAAAGTGGAT TACCATCTGT AGACCTGCAG 
AGCAGTGGCT TTGTACCTGG AGAAAGTGGC CTTCCCTCAG GGGATGTGAG CGGGTTGCCT 
TCTGGCATTG TTGATATCAG CGGCTTGCCT TCTGCAGAGG AAGAGGTAAC GGTGTCTGTT 
TCGAGGATAC CAGAAGTTAG TGGAATGCCA TCTGGAGCTG AAAGCAGTGG TCTGCATTCT 
GGATTTAGTG GAGAAATCTC TGGCACTGAG CTTATCAGTG GCCTGCCATC TGGAGAGGAA 
AGTGGGCTCG CCTCTGGTTT TCCCACCATC TCCCTCGTGG ATTCCACTTT GGTGGAGGTT 
GTAACGGCAG CACCGGGACG GCAAGAGGAG GGAAAAGGAT CAATTGGAGT CAGTGGTGAA 
GAAGAGCTGT CAGGGTTTCC GTCTGCAGAG TGGGACAGCA GTGGGGCCAG AGGGCTGCCC 
TCGGGAGCTG AAACCAGTGG GGAGCAATCC GGGGTGCCCG AGCTCAGTGG GGAGCATTCC 
GGGGTGCCTG GGCTCAGTGG AGAGCCTTTT GAGGTGCCTG AGCTCAGCGG GGAGCATTCC 
GGGGTGACTG AGCTCAGTGG GGAGCATTCT GGGCTTCCTG AGCTCAGTGG AGAGCCTTTT 
GGGGTGCCTG AGCTCAGCGG GTTTCCCTCC GGACTGGACA TCAGTGGGGA ACCATCTGGA 
GCACCCGAGG TGAGCGGCCC GGTGGACGTG AGCGGCCTTA CCTCTGGTGT TGATGGAAGT 
GGTGAGGTCT CAGGCGTTAC CTTTATAAGC ACCAGCCTGC AAGAAGTGAC AACCCAGTCA 
GTTGCAGAAG CAGAAGCAAA AGAAATTCTA GAGATCAGTG GACTGCCTTC AGGAGAGACA 
TCAGGCATGG TGTCTGGGAG CTTAGATGTC AGCGGTCAGC CTTCGGGACA CATAGACTTT 
GGTGGGAGCG CTTCTGGAGT GCTGGAGATG AGCGGATTTC CAAGCGGAGC GGTTGAGAGC 
AGTGGAGAAG CCTCTGGGGT TGAAGTCACC AGTGTCCTCG CGTCTGGAGA GGAAAGTGGG 
CTCACCTCAG GCTTTCCCAC CGTGTCTCTC GTGGATACCA CGTTGGTAGA AGTTGTAACG 
CAGACATCAG TTGCTCAAGA GGTGGGAGAA GGACCATCTG GGATGATAGA AATCAGTGGA 
TTTCTTTCTG GAGACAGAGG AGTATCTGGA GAAGGGTCTG GAGCTGTGCA GTCTAGTGGG 
CTTCCTTCAG GAACAGGAGA CTTCAGCGGA GAGCCATCCG GGATCCCGTA TTTCAGCGGA 
GACATTTCTG GAGCCACAGA TCTAAGCGGA CAACCTTCCG CAGTGACTGA TATTAGTGGG 
GAGGACTCGG GACTTCCAGA AGTCACTTTA GTCACATCCG ATTTAGTAGA AGTTGTGACA 
AGGCCAACAG TTTCACAGGA GCTGGGTGGG GAAACAGCTG TGACGTTTCC CTATGTCTTT 
GGGCCAAGTG GTGAGGGCTC TGCATCTGGA GACCTGAGTG GGGGAGCATC TGCAGAAGGT 
GGTATAGAAA CATCAGCAGC TTATGAAATC AGTGGTGAGA GCTCTGCCTT CCCTGAAACG 
AGTATAGAAA CATCCACAGA TCAAGAAATC AGTGGGGAAG CATCTGCATA CCCTGAGATT 
AGCGTAGAAA CATCCACGCA TCTGGAAACC AGTGGGGAAA CATCTGCATA CCCTGAGATT 
AGCACAGAGA CGTCCACCAT TCAGGAAGTT AGTGGGGAAA CGTCTGCCTT TCCTGAAATT 
AGCACAGAAA CATCCACAAT ACAAGAGATC AGTGGGGAAA CATCCGCATT TCCTGAAATT 
AGAATAGAAA CATCCACCTT TCAAGAAATC AGTGGGGAAA CGTCCGCATT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGCGGCGAAA CGTCTGCGTT TCCCGAGATT 
ACCATAGAAG CCTCCACAGT CCATGAAACC AGTGGAGAAA CATCTGCCTT TCCCGAAATT 
AGCATAGAAA CATCCACAGT CCATGAAATC AGTGGGGAAA GTTCTGCCTT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGGGGTGAAA CATCTGCGTT CCCTGAGATT 
ACCATAGAAG CCTCCACCAT TCAAGAGATC AGTGGAGAAA CATCTGCATT TCCTGAAATT 
AGCATAGCAA CTTCCACCGT CCGTGAAATC AGCGGCGAAA CGTCTGCCTT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGGGGTGAAA CATCTGCATT GCCTGAGATT 
ACCGTAGAGA CATCCACTGT CCATGAAACC AGTGGGGAAG CGTCTGCCTT TCCTGAAATT 
AGCATAGAAA CTTCCACAAG ACAAGAAGCC AGGGGTGAAG CATCCGCCTA CCCTGAGGTT 
AGCATAGAGG CATCCACAAC TCAAGAAGTA AGTGGGGAAA GTTCTGCCTT CCCTGAAATT 
AGCGTAGAGA CATCCACAAG TCAGGAAGCC CGCGGTGAAA CATCTGCCTT TCCTGAGATT 
GGCATAGAGA CATCCACAGC CCACGAAGGC AGTGGGGAAA CTCCTGGGCT GCCTGCTGTT 
AGCACTGACA CTGCTGCCAC GTCTCTGGCC AGTGGTGAGC CCTCCGGTGC TCCTGAGAAG 
GAAACTCCCG ACACAACATC ACATCTGATC ACGGGCGTTT CAGGGGAAAC CTCTGTCCCA 
GATGCTGTAA TCAGTACCAG TGCTCCAGAT GTTGAACTAG CGCAGGGACC CAGAAACACT 
GAAGAGACTC AGCTTGAAAT AGAGCCCTCC ACTCCTGCGG CATCTGGACA AGAGACAGAA 
ACAGCTGCTG TCCTCGACAA TCCCCATCTG CCAGCCACTG CTACTGCTGC CCTGCATCCA 
GCCTCCCAAG AAGCAGTAGA TGCACTGGGA CCCACGACAG AAGACCTGGC AAACTGTGAG 
GAAGGCTGGA TCAAGTTCCA GGGCCACTGC TACAGGCACT TTGAAGAGAG GGAGACGTGG 
ATGGACGCAG AGTCCAGGTG CAGAGAACAT CAAGCCCACC TGAGCAGCAT CATTACTCCA 
GAGGAACAAG AATTTGTGAA CAGCCATGCA CAAGACTATC AGTGGATCGG CCTCAGTGAC 
AGAGCCGTGG AGAATGATTT CCGCTGGTCT GATGGACACT CGCTGCAATT TGAGAACTGG 
CGGCCCAACC AACCTGACAA CTTCTTCTCC GCGGGCGAGG ACTGCGTTGT GATGATCTGG 
CATGAGCAAG GCGAATGGAA CGACGTCCCC TGCAACTATC ACCTGCCTTT CACGTGCAAG 
AAGGGAACAG TTGCCTGTGG GGACCCACCT GTAGTGGAGA ACGCGCGGAC CTTTGGCCGG 
AAGAAGGACC GCTATGAGAT CAACTCCCTG GTGCGGTACC AGTGTGACCA CGGCTACATC 
CAGCGCCACG TGCCCACCAT CCGCTGCCAA CCCAACGGGC ACTGGGAGGA ACCGCGGATA 
TCCTGCACAA ACCCCTCCAG CTACCAACGC CGGCTATACA AGAGGAGCCC CCGGAGCCGG 
TTGAGACCCG GTGTCGTGCA CAGACCCACC CATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015147465.1
CDS	1492..7647
Translation	MTTLLLVFVCLQAITTAASAELSDSSDGLEVKIPEQSPLRVVLGSSLNIPCYFNIPEEEDTNALLTPRIKWSKLSNGTEIVLLVATGGKIRLNAEYREVISLPNYPAIPTDATLEIKALRSNHTGIYRCEVMYGIEDRQDTIEVLVKGVVFHYRAISTRYTLNFERAKQACIQNSAVIATPEQLQAAYEDGYEQCDAGWLADQTVRYPIHLPRERCYGDKDEFPGVRTYGVRETDETYDVYCYAEQMQGKVFYATSPEKFTFQEAFDKCHSLGARLATTGELYLAWKDGMDMCSAGWLADRSVRYPISRARPNCGGNLVGVRTVYLNPANQTGYPHPSSRYDAICYSGDDFEALVPGLFTDEVGTELGSAFTIQTVTQTEVELPLPRNVTEEEARGSIATLEPMEITATATELYEAFTVLPDLFATSVTVETASPREENVTREEITGIWAVPEEVTTSVSGTAFTTGMAEVSSVEEAIAVTATPGLESASPFTIEDHLVQVTAAPDVALLPRQPISPTGVVFHYRAATSRYAFSFIQAQQACLENNAVIATPEQLQAAYEAGFDQCDAGWLRDQTVRYPIVNPRSNCVGDKESSPGVRSYGMRPASETYDVYCYIDRLKGEVFFATQPEQFTFQEAQLYCESQNATLASAGQLHAAWKQGLDRCYPGWLADGSLRYPIVSPRPACGGDAPGVRTIYQHHNQTGFPDPLSRHHAFCFRALPSVVEEGVTSLFEEEVMVTQLIPGVEGIPSGEETTVETELSSEPENQTAQGTEVFPTDVSLLSARPSAFPPATVIPEETSTNASIPEVSGEFPESGEHPTSGEPSASGAPDTSGEPTSVGFELSGEQSGIGESGLPSVDLQSSGFVPGESGLPSGDVSGLPSGIVDISGLPSAEEEVTVSVSRIPEVSGMPSGAESSGLHSGFSGEISGTELISGLPSGEESGLASGFPTISLVDSTLVEVVTAAPGRQEEGKGSIGVSGEEELSGFPSAEWDSSGARGLPSGAETSGEQSGVPELSGEHSGVPGLSGEPFEVPELSGEHSGVTELSGEHSGLPELSGEPFGVPELSGFPSGLDISGEPSGAPEVSGPVDVSGLTSGVDGSGEVSGVTFISTSLQEVTTQSVAEAEAKEILEISGLPSGETSGMVSGSLDVSGQPSGHIDFGGSASGVLEMSGFPSGAVESSGEASGVEVTSVLASGEESGLTSGFPTVSLVDTTLVEVVTQTSVAQEVGEGPSGMIEISGFLSGDRGVSGEGSGAVQSSGLPSGTGDFSGEPSGIPYFSGDISGATDLSGQPSAVTDISGEDSGLPEVTLVTSDLVEVVTRPTVSQELGGETAVTFPYVFGPSGEGSASGDLSGGASAEGGIETSAAYEISGESSAFPETSIETSTDQEISGEASAYPEISVETSTHLETSGETSAYPEISTETSTIQEVSGETSAFPEISTETSTIQEISGETSAFPEIRIETSTFQEISGETSAFPEIRIETSTSQEARGETSAFPEITIEASTVHETSGETSAFPEISIETSTVHEISGESSAFPEIRIETSTSQEARGETSAFPEITIEASTIQEISGETSAFPEISIATSTVREISGETSAFPEIRIETSTSQEARGETSALPEITVETSTVHETSGEASAFPEISIETSTRQEARGEASAYPEVSIEASTTQEVSGESSAFPEISVETSTSQEARGETSAFPEIGIETSTAHEGSGETPGLPAVSTDTAATSLASGEPSGAPEKETPDTTSHLITGVSGETSVPDAVISTSAPDVELAQGPRNTEETQLEIEPSTPAASGQETETAAVLDNPHLPATATAALHPASQEAVDALGPTTEDLANCEEGWIKFQGHCYRHFEERETWMDAESRCREHQAHLSSIITPEEQEFVNSHAQDYQWIGLSDRAVENDFRWSDGHSLQFENWRPNQPDNFFSAGEDCVVMIWHEQGEWNDVPCNYHLPFTCKKGTVACGDPPVVENARTFGRKKDRYEINSLVRYQCDHGYIQRHVPTIRCQPNGHWEEPRISCTNPSSYQRRLYKRSPRSRLRPGVVHRPTH

Target ORF information:

RefSeq Version	XM_015291979.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus aggrecan (ACAN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291979.2

ORF Insert Sequence:

ATGACCACTC TACTACTAGT GTTTGTGTGT TTGCAAGCCA TCACCACAGC TGCCTCCGCA 
GAGCTCTCAG ACAGTAGCGA TGGCCTGGAA GTGAAGATAC CTGAGCAGTC TCCCCTGCGT 
GTTGTCCTGG GAAGCTCCCT GAACATCCCC TGCTATTTCA ACATCCCAGA GGAAGAGGAC 
ACCAATGCTC TGCTGACCCC CCGGATCAAA TGGAGCAAGC TTTCCAATGG AACAGAGATT 
GTCTTACTAG TGGCCACCGG TGGGAAGATC CGGCTCAATG CAGAGTACAG AGAGGTGATC 
TCCTTGCCCA ATTACCCTGC CATCCCCACT GATGCCACCT TGGAAATCAA GGCGCTGAGA 
TCCAACCACA CTGGGATTTA TCGCTGTGAA GTGATGTATG GGATTGAGGA CAGACAAGAC 
ACCATAGAGG TCCTGGTGAA AGGCGTCGTG TTCCACTACA GAGCAATCTC CACAAGGTAC 
ACTTTGAACT TCGAGAGGGC AAAGCAGGCC TGTATCCAGA ACAGTGCTGT CATTGCCACC 
CCTGAGCAGC TGCAGGCTGC CTACGAGGAT GGGTACGAGC AGTGCGATGC CGGCTGGCTG 
GCTGATCAGA CTGTCAGGTA CCCCATCCAT CTGCCCCGGG AGCGCTGCTA CGGTGACAAG 
GATGAGTTTC CAGGAGTGAG AACCTACGGT GTCCGTGAGA CAGATGAAAC CTATGATGTT 
TACTGCTATG CAGAGCAAAT GCAAGGCAAA GTCTTCTACG CCACCTCCCC CGAGAAGTTC 
ACCTTCCAGG AAGCTTTTGA CAAATGCCAC AGCTTGGGAG CCCGCCTGGC CACCACGGGC 
GAGCTGTACT TGGCCTGGAA GGATGGCATG GACATGTGCA GTGCGGGCTG GCTGGCTGAC 
CGCAGCGTTC GCTACCCCAT TTCCAGAGCA CGGCCCAATT GTGGAGGGAA CCTGGTGGGC 
GTGCGGACCG TTTACCTGAA CCCTGCCAAC CAGACAGGGT ACCCTCACCC CAGCTCACGC 
TACGATGCCA TCTGTTACAG TGGTGATGAC TTCGAGGCTC TGGTCCCAGG GCTGTTCACT 
GACGAGGTTG GGACTGAGCT GGGCAGTGCT TTTACCATAC AGACCGTCAC ACAGACTGAG 
GTGGAGCTGC CCCTGCCACG CAATGTCACA GAAGAGGAGG CCCGTGGCAG CATCGCTACC 
CTGGAGCCCA TGGAGATCAC AGCCACCGCC ACTGAGCTGT ATGAGGCCTT CACCGTCCTG 
CCTGACCTCT TTGCCACCAG TGTCACAGTA GAGACAGCTT CCCCAAGGGA AGAGAACGTG 
ACCAGAGAGG AGATCACAGG GATATGGGCT GTGCCTGAAG AGGTCACTAC ATCGGTCTCA 
GGCACTGCTT TCACCACCGG AATGGCAGAG GTGAGCTCAG TGGAAGAGGC CATAGCAGTG 
ACTGCCACAC CAGGACTGGA GTCTGCCTCT CCATTCACCA TAGAAGATCA TCTCGTGCAA 
GTGACAGCAG CCCCTGATGT TGCCCTCCTC CCCAGGCAGC CCATTTCCCC CACTGGTGTG 
GTGTTCCACT ACCGTGCTGC CACCAGCAGA TACGCCTTCT CCTTCATCCA AGCCCAGCAG 
GCCTGCCTGG AGAACAATGC CGTTATTGCC ACTCCCGAGC AGCTCCAGGC TGCCTACGAG 
GCTGGCTTTG ATCAGTGCGA TGCCGGCTGG CTGCGGGACC AGACGGTCAG GTATCCCATT 
GTGAATCCCC GCAGCAACTG TGTAGGAGAC AAAGAGAGCT CCCCAGGTGT GCGGTCATAC 
GGCATGCGCC CGGCCTCAGA GACCTACGAT GTGTACTGTT ACATCGACAG GCTAAAGGGT 
GAGGTGTTTT TTGCCACCCA GCCAGAGCAG TTCACCTTCC AAGAAGCCCA GCTGTACTGT 
GAAAGCCAAA ATGCCACGCT GGCCTCTGCT GGGCAACTCC ACGCTGCCTG GAAGCAGGGC 
CTGGATAGGT GCTACCCTGG GTGGCTGGCC GACGGCAGCT TGCGGTACCC CATTGTGAGC 
CCCCGACCTG CCTGCGGGGG GGATGCACCT GGTGTGAGGA CCATCTACCA GCACCACAAC 
CAGACGGGCT TTCCTGACCC TCTGTCACGG CATCACGCTT TCTGCTTCAG AGCTCTGCCA 
TCCGTAGTGG AGGAGGGGGT GACCTCACTC TTTGAAGAAG AGGTGATGGT AACCCAACTG 
ATCCCTGGAG TGGAAGGAAT ACCTTCTGGG GAGGAAACAA CTGTGGAGAC AGAGCTTTCC 
TCTGAGCCTG AGAATCAGAC AGCCCAGGGA ACGGAGGTCT TCCCAACTGA CGTATCACTG 
CTCTCAGCGA GACCATCTGC TTTTCCTCCA GCTACTGTAA TACCAGAGGA AACAAGTACC 
AATGCTTCCA TCCCCGAAGT GTCCGGAGAG TTCCCTGAGT CTGGAGAGCA CCCAACCAGT 
GGCGAACCTT CAGCATCTGG AGCCCCAGAC ACGAGTGGAG AACCAACTTC CGTAGGTTTT 
GAACTGAGTG GAGAGCAATC CGGGATTGGA GAAAGTGGAT TACCATCTGT AGACCTGCAG 
AGCAGTGGCT TTGTACCTGG AGAAAGTGGC CTTCCCTCAG GGGATGTGAG CGGGTTGCCT 
TCTGGCATTG TTGATATCAG CGGCTTGCCT TCTGCAGAGG AAGAGGTAAC GGTGTCTGTT 
TCGAGGATAC CAGAAGTTAG TGGAATGCCA TCTGGAGCTG AAAGCAGTGG TCTGCATTCT 
GGATTTAGTG GAGAAATCTC TGGCACTGAG CTTATCAGTG GCCTGCCATC TGGAGAGGAA 
AGTGGGCTCG CCTCTGGTTT TCCCACCATC TCCCTCGTGG ATTCCACTTT GGTGGAGGTT 
GTAACGGCAG CACCGGGACG GCAAGAGGAG GGAAAAGGAT CAATTGGAGT CAGTGGTGAA 
GAAGAGCTGT CAGGGTTTCC GTCTGCAGAG TGGGACAGCA GTGGGGCCAG AGGGCTGCCC 
TCGGGAGCTG AAACCAGTGG GGAGCAATCC GGGGTGCCCG AGCTCAGTGG GGAGCATTCC 
GGGGTGCCTG GGCTCAGTGG AGAGCCTTTT GAGGTGCCTG AGCTCAGCGG GGAGCATTCC 
GGGGTGACTG AGCTCAGTGG GGAGCATTCT GGGCTTCCTG AGCTCAGTGG AGAGCCTTTT 
GGGGTGCCTG AGCTCAGCGG GTTTCCCTCC GGACTGGACA TCAGTGGGGA ACCATCTGGA 
GCACCCGAGG TGAGCGGCCC GGTGGACGTG AGCGGCCTTA CCTCTGGTGT TGATGGAAGT 
GGTGAGGTCT CAGGCGTTAC CTTTATAAGC ACCAGCCTGC AAGAAGTGAC AACCCAGTCA 
GTTGCAGAAG CAGAAGCAAA AGAAATTCTA GAGATCAGTG GACTGCCTTC AGGAGAGACA 
TCAGGCATGG TGTCTGGGAG CTTAGATGTC AGCGGTCAGC CTTCGGGACA CATAGACTTT 
GGTGGGAGCG CTTCTGGAGT GCTGGAGATG AGCGGATTTC CAAGCGGAGC GGTTGAGAGC 
AGTGGAGAAG CCTCTGGGGT TGAAGTCACC AGTGTCCTCG CGTCTGGAGA GGAAAGTGGG 
CTCACCTCAG GCTTTCCCAC CGTGTCTCTC GTGGATACCA CGTTGGTAGA AGTTGTAACG 
CAGACATCAG TTGCTCAAGA GGTGGGAGAA GGACCATCTG GGATGATAGA AATCAGTGGA 
TTTCTTTCTG GAGACAGAGG AGTATCTGGA GAAGGGTCTG GAGCTGTGCA GTCTAGTGGG 
CTTCCTTCAG GAACAGGAGA CTTCAGCGGA GAGCCATCCG GGATCCCGTA TTTCAGCGGA 
GACATTTCTG GAGCCACAGA TCTAAGCGGA CAACCTTCCG CAGTGACTGA TATTAGTGGG 
GAGGACTCGG GACTTCCAGA AGTCACTTTA GTCACATCCG ATTTAGTAGA AGTTGTGACA 
AGGCCAACAG TTTCACAGGA GCTGGGTGGG GAAACAGCTG TGACGTTTCC CTATGTCTTT 
GGGCCAAGTG GTGAGGGCTC TGCATCTGGA GACCTGAGTG GGGGAGCATC TGCAGAAGGT 
GGTATAGAAA CATCAGCAGC TTATGAAATC AGTGGTGAGA GCTCTGCCTT CCCTGAAACG 
AGTATAGAAA CATCCACAGA TCAAGAAATC AGTGGGGAAG CATCTGCATA CCCTGAGATT 
AGCGTAGAAA CATCCACGCA TCTGGAAACC AGTGGGGAAA CATCTGCATA CCCTGAGATT 
AGCACAGAGA CGTCCACCAT TCAGGAAGTT AGTGGGGAAA CGTCTGCCTT TCCTGAAATT 
AGCACAGAAA CATCCACAAT ACAAGAGATC AGTGGGGAAA CATCCGCATT TCCTGAAATT 
AGAATAGAAA CATCCACCTT TCAAGAAATC AGTGGGGAAA CGTCCGCATT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGCGGCGAAA CGTCTGCGTT TCCCGAGATT 
ACCATAGAAG CCTCCACAGT CCATGAAACC AGTGGAGAAA CATCTGCCTT TCCCGAAATT 
AGCATAGAAA CATCCACAGT CCATGAAATC AGTGGGGAAA GTTCTGCCTT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGGGGTGAAA CATCTGCGTT CCCTGAGATT 
ACCATAGAAG CCTCCACCAT TCAAGAGATC AGTGGAGAAA CATCTGCATT TCCTGAAATT 
AGCATAGCAA CTTCCACCGT CCGTGAAATC AGCGGCGAAA CGTCTGCCTT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGGGGTGAAA CATCTGCATT GCCTGAGATT 
ACCGTAGAGA CATCCACTGT CCATGAAACC AGTGGGGAAG CGTCTGCCTT TCCTGAAATT 
AGCATAGAAA CTTCCACAAG ACAAGAAGCC AGGGGTGAAG CATCCGCCTA CCCTGAGGTT 
AGCATAGAGG CATCCACAAC TCAAGAAGTA AGTGGGGAAA GTTCTGCCTT CCCTGAAATT 
AGCGTAGAGA CATCCACAAG TCAGGAAGCC CGCGGTGAAA CATCTGCCTT TCCTGAGATT 
GGCATAGAGA CATCCACAGC CCACGAAGGC AGTGGGGAAA CTCCTGGGCT GCCTGCTGTT 
AGCACTGACA CTGCTGCCAC GTCTCTGGCC AGTGGTGAGC CCTCCGGTGC TCCTGAGAAG 
GAAACTCCCG ACACAACATC ACATCTGATC ACGGGCGTTT CAGGGGAAAC CTCTGTCCCA 
GATGCTGTAA TCAGTACCAG TGCTCCAGAT GTTGAACTAG CGCAGGGACC CAGAAACACT 
GAAGAGACTC AGCTTGAAAT AGAGCCCTCC ACTCCTGCGG CATCTGGACA AGAGACAGAA 
ACAGCTGCTG TCCTCGACAA TCCCCATCTG CCAGCCACTG CTACTGCTGC CCTGCATCCA 
GCCTCCCAAG AAGCAGTAGA TGCACTGGGA CCCACGACAG AAGACCTGGC AAACTGTGAG 
GAAGGCTGGA TCAAGTTCCA GGGCCACTGC TACAGGCACT TTGAAGAGAG GGAGACGTGG 
ATGGACGCAG AGTCCAGGTG CAGAGAACAT CAAGCCCACC TGAGCAGCAT CATTACTCCA 
GAGGAACAAG AATTTGTGAA CAGCCATGCA CAAGACTATC AGTGGATCGG CCTCAGTGAC 
AGAGCCGTGG AGAATGATTT CCGCTGGTCT GATGGACACT CGCTGCAATT TGAGAACTGG 
CGGCCCAACC AACCTGACAA CTTCTTCTCC GCGGGCGAGG ACTGCGTTGT GATGATCTGG 
CATGAGCAAG GCGAATGGAA CGACGTCCCC TGCAACTATC ACCTGCCTTT CACGTGCAAG 
AAGGGAACAG TTGCCTGTGG GGACCCACCT GTAGTGGAGA ACGCGCGGAC CTTTGGCCGG 
AAGAAGGACC GCTATGAGAT CAACTCCCTG GTGCGGTACC AGTGTGACCA CGGCTACATC 
CAGCGCCACG TGCCCACCAT CCGCTGCCAA CCCAACGGGC ACTGGGAGGA ACCGCGGATA 
TCCTGCACAA ACCCCTCCAG CTACCAACGC CGGCTATACA AGAGGAGCCC CCGGAGCCGG 
TTGAGACCCG GTGTCGTGCA CAGACCCACC CATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACAN ( XM_015291979.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015147465.1 Gallus gallus aggrecan (ACAN), transcript variant X3, mRNA.

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CloneID	OGa40533
Clone ID Related Accession (Same CDS sequence)	XM_015291979.2 , XM_015291979.1
Accession Version	XM_015291979.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6156bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	aggrecan core protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455924.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACTC TACTACTAGT GTTTGTGTGT TTGCAAGCCA TCACCACAGC TGCCTCCGCA 
GAGCTCTCAG ACAGTAGCGA TGGCCTGGAA GTGAAGATAC CTGAGCAGTC TCCCCTGCGT 
GTTGTCCTGG GAAGCTCCCT GAACATCCCC TGCTATTTCA ACATCCCAGA GGAAGAGGAC 
ACCAATGCTC TGCTGACCCC CCGGATCAAA TGGAGCAAGC TTTCCAATGG AACAGAGATT 
GTCTTACTAG TGGCCACCGG TGGGAAGATC CGGCTCAATG CAGAGTACAG AGAGGTGATC 
TCCTTGCCCA ATTACCCTGC CATCCCCACT GATGCCACCT TGGAAATCAA GGCGCTGAGA 
TCCAACCACA CTGGGATTTA TCGCTGTGAA GTGATGTATG GGATTGAGGA CAGACAAGAC 
ACCATAGAGG TCCTGGTGAA AGGCGTCGTG TTCCACTACA GAGCAATCTC CACAAGGTAC 
ACTTTGAACT TCGAGAGGGC AAAGCAGGCC TGTATCCAGA ACAGTGCTGT CATTGCCACC 
CCTGAGCAGC TGCAGGCTGC CTACGAGGAT GGGTACGAGC AGTGCGATGC CGGCTGGCTG 
GCTGATCAGA CTGTCAGGTA CCCCATCCAT CTGCCCCGGG AGCGCTGCTA CGGTGACAAG 
GATGAGTTTC CAGGAGTGAG AACCTACGGT GTCCGTGAGA CAGATGAAAC CTATGATGTT 
TACTGCTATG CAGAGCAAAT GCAAGGCAAA GTCTTCTACG CCACCTCCCC CGAGAAGTTC 
ACCTTCCAGG AAGCTTTTGA CAAATGCCAC AGCTTGGGAG CCCGCCTGGC CACCACGGGC 
GAGCTGTACT TGGCCTGGAA GGATGGCATG GACATGTGCA GTGCGGGCTG GCTGGCTGAC 
CGCAGCGTTC GCTACCCCAT TTCCAGAGCA CGGCCCAATT GTGGAGGGAA CCTGGTGGGC 
GTGCGGACCG TTTACCTGAA CCCTGCCAAC CAGACAGGGT ACCCTCACCC CAGCTCACGC 
TACGATGCCA TCTGTTACAG TGGTGATGAC TTCGAGGCTC TGGTCCCAGG GCTGTTCACT 
GACGAGGTTG GGACTGAGCT GGGCAGTGCT TTTACCATAC AGACCGTCAC ACAGACTGAG 
GTGGAGCTGC CCCTGCCACG CAATGTCACA GAAGAGGAGG CCCGTGGCAG CATCGCTACC 
CTGGAGCCCA TGGAGATCAC AGCCACCGCC ACTGAGCTGT ATGAGGCCTT CACCGTCCTG 
CCTGACCTCT TTGCCACCAG TGTCACAGTA GAGACAGCTT CCCCAAGGGA AGAGAACGTG 
ACCAGAGAGG AGATCACAGG GATATGGGCT GTGCCTGAAG AGGTCACTAC ATCGGTCTCA 
GGCACTGCTT TCACCACCGG AATGGCAGAG GTGAGCTCAG TGGAAGAGGC CATAGCAGTG 
ACTGCCACAC CAGGACTGGA GTCTGCCTCT CCATTCACCA TAGAAGATCA TCTCGTGCAA 
GTGACAGCAG CCCCTGATGT TGCCCTCCTC CCCAGGCAGC CCATTTCCCC CACTGGTGTG 
GTGTTCCACT ACCGTGCTGC CACCAGCAGA TACGCCTTCT CCTTCATCCA AGCCCAGCAG 
GCCTGCCTGG AGAACAATGC CGTTATTGCC ACTCCCGAGC AGCTCCAGGC TGCCTACGAG 
GCTGGCTTTG ATCAGTGCGA TGCCGGCTGG CTGCGGGACC AGACGGTCAG GTATCCCATT 
GTGAATCCCC GCAGCAACTG TGTAGGAGAC AAAGAGAGCT CCCCAGGTGT GCGGTCATAC 
GGCATGCGCC CGGCCTCAGA GACCTACGAT GTGTACTGTT ACATCGACAG GCTAAAGGGT 
GAGGTGTTTT TTGCCACCCA GCCAGAGCAG TTCACCTTCC AAGAAGCCCA GCTGTACTGT 
GAAAGCCAAA ATGCCACGCT GGCCTCTGCT GGGCAACTCC ACGCTGCCTG GAAGCAGGGC 
CTGGATAGGT GCTACCCTGG GTGGCTGGCC GACGGCAGCT TGCGGTACCC CATTGTGAGC 
CCCCGACCTG CCTGCGGGGG GGATGCACCT GGTGTGAGGA CCATCTACCA GCACCACAAC 
CAGACGGGCT TTCCTGACCC TCTGTCACGG CATCACGCTT TCTGCTTCAG AGCTCTGCCA 
TCCGTAGTGG AGGAGGGGGT GACCTCACTC TTTGAAGAAG AGGTGATGGT AACCCAACTG 
ATCCCTGGAG TGGAAGGAAT ACCTTCTGGG GAGGAAACAA CTGTGGAGAC AGAGCTTTCC 
TCTGAGCCTG AGAATCAGAC AGCCCAGGGA ACGGAGGTCT TCCCAACTGA CGTATCACTG 
CTCTCAGCGA GACCATCTGC TTTTCCTCCA GCTACTGTAA TACCAGAGGA AACAAGTACC 
AATGCTTCCA TCCCCGAAGT GTCCGGAGAG TTCCCTGAGT CTGGAGAGCA CCCAACCAGT 
GGCGAACCTT CAGCATCTGG AGCCCCAGAC ACGAGTGGAG AACCAACTTC CGTAGGTTTT 
GAACTGAGTG GAGAGCAATC CGGGATTGGA GAAAGTGGAT TACCATCTGT AGACCTGCAG 
AGCAGTGGCT TTGTACCTGG AGAAAGTGGC CTTCCCTCAG GGGATGTGAG CGGGTTGCCT 
TCTGGCATTG TTGATATCAG CGGCTTGCCT TCTGCAGAGG AAGAGGTAAC GGTGTCTGTT 
TCGAGGATAC CAGAAGTTAG TGGAATGCCA TCTGGAGCTG AAAGCAGTGG TCTGCATTCT 
GGATTTAGTG GAGAAATCTC TGGCACTGAG CTTATCAGTG GCCTGCCATC TGGAGAGGAA 
AGTGGGCTCG CCTCTGGTTT TCCCACCATC TCCCTCGTGG ATTCCACTTT GGTGGAGGTT 
GTAACGGCAG CACCGGGACG GCAAGAGGAG GGAAAAGGAT CAATTGGAGT CAGTGGTGAA 
GAAGAGCTGT CAGGGTTTCC GTCTGCAGAG TGGGACAGCA GTGGGGCCAG AGGGCTGCCC 
TCGGGAGCTG AAACCAGTGG GGAGCAATCC GGGGTGCCCG AGCTCAGTGG GGAGCATTCC 
GGGGTGCCTG GGCTCAGTGG AGAGCCTTTT GAGGTGCCTG AGCTCAGCGG GGAGCATTCC 
GGGGTGACTG AGCTCAGTGG GGAGCATTCT GGGCTTCCTG AGCTCAGTGG AGAGCCTTTT 
GGGGTGCCTG AGCTCAGCGG GTTTCCCTCC GGACTGGACA TCAGTGGGGA ACCATCTGGA 
GCACCCGAGG TGAGCGGCCC GGTGGACGTG AGCGGCCTTA CCTCTGGTGT TGATGGAAGT 
GGTGAGGTCT CAGGCGTTAC CTTTATAAGC ACCAGCCTGC AAGAAGTGAC AACCCAGTCA 
GTTGCAGAAG CAGAAGCAAA AGAAATTCTA GAGATCAGTG GACTGCCTTC AGGAGAGACA 
TCAGGCATGG TGTCTGGGAG CTTAGATGTC AGCGGTCAGC CTTCGGGACA CATAGACTTT 
GGTGGGAGCG CTTCTGGAGT GCTGGAGATG AGCGGATTTC CAAGCGGAGC GGTTGAGAGC 
AGTGGAGAAG CCTCTGGGGT TGAAGTCACC AGTGTCCTCG CGTCTGGAGA GGAAAGTGGG 
CTCACCTCAG GCTTTCCCAC CGTGTCTCTC GTGGATACCA CGTTGGTAGA AGTTGTAACG 
CAGACATCAG TTGCTCAAGA GGTGGGAGAA GGACCATCTG GGATGATAGA AATCAGTGGA 
TTTCTTTCTG GAGACAGAGG AGTATCTGGA GAAGGGTCTG GAGCTGTGCA GTCTAGTGGG 
CTTCCTTCAG GAACAGGAGA CTTCAGCGGA GAGCCATCCG GGATCCCGTA TTTCAGCGGA 
GACATTTCTG GAGCCACAGA TCTAAGCGGA CAACCTTCCG CAGTGACTGA TATTAGTGGG 
GAGGACTCGG GACTTCCAGA AGTCACTTTA GTCACATCCG ATTTAGTAGA AGTTGTGACA 
AGGCCAACAG TTTCACAGGA GCTGGGTGGG GAAACAGCTG TGACGTTTCC CTATGTCTTT 
GGGCCAAGTG GTGAGGGCTC TGCATCTGGA GACCTGAGTG GGGGAGCATC TGCAGAAGGT 
GGTATAGAAA CATCAGCAGC TTATGAAATC AGTGGTGAGA GCTCTGCCTT CCCTGAAACG 
AGTATAGAAA CATCCACAGA TCAAGAAATC AGTGGGGAAG CATCTGCATA CCCTGAGATT 
AGCGTAGAAA CATCCACGCA TCTGGAAACC AGTGGGGAAA CATCTGCATA CCCTGAGATT 
AGCACAGAGA CGTCCACCAT TCAGGAAGTT AGTGGGGAAA CGTCTGCCTT TCCTGAAATT 
AGCACAGAAA CATCCACAAT ACAAGAGATC AGTGGGGAAA CATCCGCATT TCCTGAAATT 
AGAATAGAAA CATCCACCTT TCAAGAAATC AGTGGGGAAA CGTCCGCATT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGCGGCGAAA CGTCTGCGTT TCCCGAGATT 
ACCATAGAAG CCTCCACAGT CCATGAAACC AGTGGAGAAA CATCTGCCTT TCCCGAAATT 
AGCATAGAAA CATCCACAGT CCATGAAATC AGTGGGGAAA GTTCTGCCTT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGGGGTGAAA CATCTGCGTT CCCTGAGATT 
ACCATAGAAG CCTCCACCAT TCAAGAGATC AGTGGAGAAA CATCTGCATT TCCTGAAATT 
AGCATAGCAA CTTCCACCGT CCGTGAAATC AGCGGCGAAA CGTCTGCCTT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGGGGTGAAA CATCTGCATT GCCTGAGATT 
ACCGTAGAGA CATCCACTGT CCATGAAACC AGTGGGGAAG CGTCTGCCTT TCCTGAAATT 
AGCATAGAAA CTTCCACAAG ACAAGAAGCC AGGGGTGAAG CATCCGCCTA CCCTGAGGTT 
AGCATAGAGG CATCCACAAC TCAAGAAGTA AGTGGGGAAA GTTCTGCCTT CCCTGAAATT 
AGCGTAGAGA CATCCACAAG TCAGGAAGCC CGCGGTGAAA CATCTGCCTT TCCTGAGATT 
GGCATAGAGA CATCCACAGC CCACGAAGGC AGTGGGGAAA CTCCTGGGCT GCCTGCTGTT 
AGCACTGACA CTGCTGCCAC GTCTCTGGCC AGTGGTGAGC CCTCCGGTGC TCCTGAGAAG 
GAAACTCCCG ACACAACATC ACATCTGATC ACGGGCGTTT CAGGGGAAAC CTCTGTCCCA 
GATGCTGTAA TCAGTACCAG TGCTCCAGAT GTTGAACTAG CGCAGGGACC CAGAAACACT 
GAAGAGACTC AGCTTGAAAT AGAGCCCTCC ACTCCTGCGG CATCTGGACA AGAGACAGAA 
ACAGCTGCTG TCCTCGACAA TCCCCATCTG CCAGCCACTG CTACTGCTGC CCTGCATCCA 
GCCTCCCAAG AAGCAGTAGA TGCACTGGGA CCCACGACAG AAGACCTGGC AAACTGTGAG 
GAAGGCTGGA TCAAGTTCCA GGGCCACTGC TACAGGCACT TTGAAGAGAG GGAGACGTGG 
ATGGACGCAG AGTCCAGGTG CAGAGAACAT CAAGCCCACC TGAGCAGCAT CATTACTCCA 
GAGGAACAAG AATTTGTGAA CAGCCATGCA CAAGACTATC AGTGGATCGG CCTCAGTGAC 
AGAGCCGTGG AGAATGATTT CCGCTGGTCT GATGGACACT CGCTGCAATT TGAGAACTGG 
CGGCCCAACC AACCTGACAA CTTCTTCTCC GCGGGCGAGG ACTGCGTTGT GATGATCTGG 
CATGAGCAAG GCGAATGGAA CGACGTCCCC TGCAACTATC ACCTGCCTTT CACGTGCAAG 
AAGGGAACAG TTGCCTGTGG GGACCCACCT GTAGTGGAGA ACGCGCGGAC CTTTGGCCGG 
AAGAAGGACC GCTATGAGAT CAACTCCCTG GTGCGGTACC AGTGTGACCA CGGCTACATC 
CAGCGCCACG TGCCCACCAT CCGCTGCCAA CCCAACGGGC ACTGGGAGGA ACCGCGGATA 
TCCTGCACAA ACCCCTCCAG CTACCAACGC CGGCTATACA AGAGGAGCCC CCGGAGCCGG 
TTGAGACCCG GTGTCGTGCA CAGACCCACC CATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015147465.1
CDS	1483..7638
Translation	MTTLLLVFVCLQAITTAASAELSDSSDGLEVKIPEQSPLRVVLGSSLNIPCYFNIPEEEDTNALLTPRIKWSKLSNGTEIVLLVATGGKIRLNAEYREVISLPNYPAIPTDATLEIKALRSNHTGIYRCEVMYGIEDRQDTIEVLVKGVVFHYRAISTRYTLNFERAKQACIQNSAVIATPEQLQAAYEDGYEQCDAGWLADQTVRYPIHLPRERCYGDKDEFPGVRTYGVRETDETYDVYCYAEQMQGKVFYATSPEKFTFQEAFDKCHSLGARLATTGELYLAWKDGMDMCSAGWLADRSVRYPISRARPNCGGNLVGVRTVYLNPANQTGYPHPSSRYDAICYSGDDFEALVPGLFTDEVGTELGSAFTIQTVTQTEVELPLPRNVTEEEARGSIATLEPMEITATATELYEAFTVLPDLFATSVTVETASPREENVTREEITGIWAVPEEVTTSVSGTAFTTGMAEVSSVEEAIAVTATPGLESASPFTIEDHLVQVTAAPDVALLPRQPISPTGVVFHYRAATSRYAFSFIQAQQACLENNAVIATPEQLQAAYEAGFDQCDAGWLRDQTVRYPIVNPRSNCVGDKESSPGVRSYGMRPASETYDVYCYIDRLKGEVFFATQPEQFTFQEAQLYCESQNATLASAGQLHAAWKQGLDRCYPGWLADGSLRYPIVSPRPACGGDAPGVRTIYQHHNQTGFPDPLSRHHAFCFRALPSVVEEGVTSLFEEEVMVTQLIPGVEGIPSGEETTVETELSSEPENQTAQGTEVFPTDVSLLSARPSAFPPATVIPEETSTNASIPEVSGEFPESGEHPTSGEPSASGAPDTSGEPTSVGFELSGEQSGIGESGLPSVDLQSSGFVPGESGLPSGDVSGLPSGIVDISGLPSAEEEVTVSVSRIPEVSGMPSGAESSGLHSGFSGEISGTELISGLPSGEESGLASGFPTISLVDSTLVEVVTAAPGRQEEGKGSIGVSGEEELSGFPSAEWDSSGARGLPSGAETSGEQSGVPELSGEHSGVPGLSGEPFEVPELSGEHSGVTELSGEHSGLPELSGEPFGVPELSGFPSGLDISGEPSGAPEVSGPVDVSGLTSGVDGSGEVSGVTFISTSLQEVTTQSVAEAEAKEILEISGLPSGETSGMVSGSLDVSGQPSGHIDFGGSASGVLEMSGFPSGAVESSGEASGVEVTSVLASGEESGLTSGFPTVSLVDTTLVEVVTQTSVAQEVGEGPSGMIEISGFLSGDRGVSGEGSGAVQSSGLPSGTGDFSGEPSGIPYFSGDISGATDLSGQPSAVTDISGEDSGLPEVTLVTSDLVEVVTRPTVSQELGGETAVTFPYVFGPSGEGSASGDLSGGASAEGGIETSAAYEISGESSAFPETSIETSTDQEISGEASAYPEISVETSTHLETSGETSAYPEISTETSTIQEVSGETSAFPEISTETSTIQEISGETSAFPEIRIETSTFQEISGETSAFPEIRIETSTSQEARGETSAFPEITIEASTVHETSGETSAFPEISIETSTVHEISGESSAFPEIRIETSTSQEARGETSAFPEITIEASTIQEISGETSAFPEISIATSTVREISGETSAFPEIRIETSTSQEARGETSALPEITVETSTVHETSGEASAFPEISIETSTRQEARGEASAYPEVSIEASTTQEVSGESSAFPEISVETSTSQEARGETSAFPEIGIETSTAHEGSGETPGLPAVSTDTAATSLASGEPSGAPEKETPDTTSHLITGVSGETSVPDAVISTSAPDVELAQGPRNTEETQLEIEPSTPAASGQETETAAVLDNPHLPATATAALHPASQEAVDALGPTTEDLANCEEGWIKFQGHCYRHFEERETWMDAESRCREHQAHLSSIITPEEQEFVNSHAQDYQWIGLSDRAVENDFRWSDGHSLQFENWRPNQPDNFFSAGEDCVVMIWHEQGEWNDVPCNYHLPFTCKKGTVACGDPPVVENARTFGRKKDRYEINSLVRYQCDHGYIQRHVPTIRCQPNGHWEEPRISCTNPSSYQRRLYKRSPRSRLRPGVVHRPTH

Target ORF information:

RefSeq Version	XM_015291979.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus aggrecan (ACAN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291979.1

ORF Insert Sequence:

ATGACCACTC TACTACTAGT GTTTGTGTGT TTGCAAGCCA TCACCACAGC TGCCTCCGCA 
GAGCTCTCAG ACAGTAGCGA TGGCCTGGAA GTGAAGATAC CTGAGCAGTC TCCCCTGCGT 
GTTGTCCTGG GAAGCTCCCT GAACATCCCC TGCTATTTCA ACATCCCAGA GGAAGAGGAC 
ACCAATGCTC TGCTGACCCC CCGGATCAAA TGGAGCAAGC TTTCCAATGG AACAGAGATT 
GTCTTACTAG TGGCCACCGG TGGGAAGATC CGGCTCAATG CAGAGTACAG AGAGGTGATC 
TCCTTGCCCA ATTACCCTGC CATCCCCACT GATGCCACCT TGGAAATCAA GGCGCTGAGA 
TCCAACCACA CTGGGATTTA TCGCTGTGAA GTGATGTATG GGATTGAGGA CAGACAAGAC 
ACCATAGAGG TCCTGGTGAA AGGCGTCGTG TTCCACTACA GAGCAATCTC CACAAGGTAC 
ACTTTGAACT TCGAGAGGGC AAAGCAGGCC TGTATCCAGA ACAGTGCTGT CATTGCCACC 
CCTGAGCAGC TGCAGGCTGC CTACGAGGAT GGGTACGAGC AGTGCGATGC CGGCTGGCTG 
GCTGATCAGA CTGTCAGGTA CCCCATCCAT CTGCCCCGGG AGCGCTGCTA CGGTGACAAG 
GATGAGTTTC CAGGAGTGAG AACCTACGGT GTCCGTGAGA CAGATGAAAC CTATGATGTT 
TACTGCTATG CAGAGCAAAT GCAAGGCAAA GTCTTCTACG CCACCTCCCC CGAGAAGTTC 
ACCTTCCAGG AAGCTTTTGA CAAATGCCAC AGCTTGGGAG CCCGCCTGGC CACCACGGGC 
GAGCTGTACT TGGCCTGGAA GGATGGCATG GACATGTGCA GTGCGGGCTG GCTGGCTGAC 
CGCAGCGTTC GCTACCCCAT TTCCAGAGCA CGGCCCAATT GTGGAGGGAA CCTGGTGGGC 
GTGCGGACCG TTTACCTGAA CCCTGCCAAC CAGACAGGGT ACCCTCACCC CAGCTCACGC 
TACGATGCCA TCTGTTACAG TGGTGATGAC TTCGAGGCTC TGGTCCCAGG GCTGTTCACT 
GACGAGGTTG GGACTGAGCT GGGCAGTGCT TTTACCATAC AGACCGTCAC ACAGACTGAG 
GTGGAGCTGC CCCTGCCACG CAATGTCACA GAAGAGGAGG CCCGTGGCAG CATCGCTACC 
CTGGAGCCCA TGGAGATCAC AGCCACCGCC ACTGAGCTGT ATGAGGCCTT CACCGTCCTG 
CCTGACCTCT TTGCCACCAG TGTCACAGTA GAGACAGCTT CCCCAAGGGA AGAGAACGTG 
ACCAGAGAGG AGATCACAGG GATATGGGCT GTGCCTGAAG AGGTCACTAC ATCGGTCTCA 
GGCACTGCTT TCACCACCGG AATGGCAGAG GTGAGCTCAG TGGAAGAGGC CATAGCAGTG 
ACTGCCACAC CAGGACTGGA GTCTGCCTCT CCATTCACCA TAGAAGATCA TCTCGTGCAA 
GTGACAGCAG CCCCTGATGT TGCCCTCCTC CCCAGGCAGC CCATTTCCCC CACTGGTGTG 
GTGTTCCACT ACCGTGCTGC CACCAGCAGA TACGCCTTCT CCTTCATCCA AGCCCAGCAG 
GCCTGCCTGG AGAACAATGC CGTTATTGCC ACTCCCGAGC AGCTCCAGGC TGCCTACGAG 
GCTGGCTTTG ATCAGTGCGA TGCCGGCTGG CTGCGGGACC AGACGGTCAG GTATCCCATT 
GTGAATCCCC GCAGCAACTG TGTAGGAGAC AAAGAGAGCT CCCCAGGTGT GCGGTCATAC 
GGCATGCGCC CGGCCTCAGA GACCTACGAT GTGTACTGTT ACATCGACAG GCTAAAGGGT 
GAGGTGTTTT TTGCCACCCA GCCAGAGCAG TTCACCTTCC AAGAAGCCCA GCTGTACTGT 
GAAAGCCAAA ATGCCACGCT GGCCTCTGCT GGGCAACTCC ACGCTGCCTG GAAGCAGGGC 
CTGGATAGGT GCTACCCTGG GTGGCTGGCC GACGGCAGCT TGCGGTACCC CATTGTGAGC 
CCCCGACCTG CCTGCGGGGG GGATGCACCT GGTGTGAGGA CCATCTACCA GCACCACAAC 
CAGACGGGCT TTCCTGACCC TCTGTCACGG CATCACGCTT TCTGCTTCAG AGCTCTGCCA 
TCCGTAGTGG AGGAGGGGGT GACCTCACTC TTTGAAGAAG AGGTGATGGT AACCCAACTG 
ATCCCTGGAG TGGAAGGAAT ACCTTCTGGG GAGGAAACAA CTGTGGAGAC AGAGCTTTCC 
TCTGAGCCTG AGAATCAGAC AGCCCAGGGA ACGGAGGTCT TCCCAACTGA CGTATCACTG 
CTCTCAGCGA GACCATCTGC TTTTCCTCCA GCTACTGTAA TACCAGAGGA AACAAGTACC 
AATGCTTCCA TCCCCGAAGT GTCCGGAGAG TTCCCTGAGT CTGGAGAGCA CCCAACCAGT 
GGCGAACCTT CAGCATCTGG AGCCCCAGAC ACGAGTGGAG AACCAACTTC CGTAGGTTTT 
GAACTGAGTG GAGAGCAATC CGGGATTGGA GAAAGTGGAT TACCATCTGT AGACCTGCAG 
AGCAGTGGCT TTGTACCTGG AGAAAGTGGC CTTCCCTCAG GGGATGTGAG CGGGTTGCCT 
TCTGGCATTG TTGATATCAG CGGCTTGCCT TCTGCAGAGG AAGAGGTAAC GGTGTCTGTT 
TCGAGGATAC CAGAAGTTAG TGGAATGCCA TCTGGAGCTG AAAGCAGTGG TCTGCATTCT 
GGATTTAGTG GAGAAATCTC TGGCACTGAG CTTATCAGTG GCCTGCCATC TGGAGAGGAA 
AGTGGGCTCG CCTCTGGTTT TCCCACCATC TCCCTCGTGG ATTCCACTTT GGTGGAGGTT 
GTAACGGCAG CACCGGGACG GCAAGAGGAG GGAAAAGGAT CAATTGGAGT CAGTGGTGAA 
GAAGAGCTGT CAGGGTTTCC GTCTGCAGAG TGGGACAGCA GTGGGGCCAG AGGGCTGCCC 
TCGGGAGCTG AAACCAGTGG GGAGCAATCC GGGGTGCCCG AGCTCAGTGG GGAGCATTCC 
GGGGTGCCTG GGCTCAGTGG AGAGCCTTTT GAGGTGCCTG AGCTCAGCGG GGAGCATTCC 
GGGGTGACTG AGCTCAGTGG GGAGCATTCT GGGCTTCCTG AGCTCAGTGG AGAGCCTTTT 
GGGGTGCCTG AGCTCAGCGG GTTTCCCTCC GGACTGGACA TCAGTGGGGA ACCATCTGGA 
GCACCCGAGG TGAGCGGCCC GGTGGACGTG AGCGGCCTTA CCTCTGGTGT TGATGGAAGT 
GGTGAGGTCT CAGGCGTTAC CTTTATAAGC ACCAGCCTGC AAGAAGTGAC AACCCAGTCA 
GTTGCAGAAG CAGAAGCAAA AGAAATTCTA GAGATCAGTG GACTGCCTTC AGGAGAGACA 
TCAGGCATGG TGTCTGGGAG CTTAGATGTC AGCGGTCAGC CTTCGGGACA CATAGACTTT 
GGTGGGAGCG CTTCTGGAGT GCTGGAGATG AGCGGATTTC CAAGCGGAGC GGTTGAGAGC 
AGTGGAGAAG CCTCTGGGGT TGAAGTCACC AGTGTCCTCG CGTCTGGAGA GGAAAGTGGG 
CTCACCTCAG GCTTTCCCAC CGTGTCTCTC GTGGATACCA CGTTGGTAGA AGTTGTAACG 
CAGACATCAG TTGCTCAAGA GGTGGGAGAA GGACCATCTG GGATGATAGA AATCAGTGGA 
TTTCTTTCTG GAGACAGAGG AGTATCTGGA GAAGGGTCTG GAGCTGTGCA GTCTAGTGGG 
CTTCCTTCAG GAACAGGAGA CTTCAGCGGA GAGCCATCCG GGATCCCGTA TTTCAGCGGA 
GACATTTCTG GAGCCACAGA TCTAAGCGGA CAACCTTCCG CAGTGACTGA TATTAGTGGG 
GAGGACTCGG GACTTCCAGA AGTCACTTTA GTCACATCCG ATTTAGTAGA AGTTGTGACA 
AGGCCAACAG TTTCACAGGA GCTGGGTGGG GAAACAGCTG TGACGTTTCC CTATGTCTTT 
GGGCCAAGTG GTGAGGGCTC TGCATCTGGA GACCTGAGTG GGGGAGCATC TGCAGAAGGT 
GGTATAGAAA CATCAGCAGC TTATGAAATC AGTGGTGAGA GCTCTGCCTT CCCTGAAACG 
AGTATAGAAA CATCCACAGA TCAAGAAATC AGTGGGGAAG CATCTGCATA CCCTGAGATT 
AGCGTAGAAA CATCCACGCA TCTGGAAACC AGTGGGGAAA CATCTGCATA CCCTGAGATT 
AGCACAGAGA CGTCCACCAT TCAGGAAGTT AGTGGGGAAA CGTCTGCCTT TCCTGAAATT 
AGCACAGAAA CATCCACAAT ACAAGAGATC AGTGGGGAAA CATCCGCATT TCCTGAAATT 
AGAATAGAAA CATCCACCTT TCAAGAAATC AGTGGGGAAA CGTCCGCATT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGCGGCGAAA CGTCTGCGTT TCCCGAGATT 
ACCATAGAAG CCTCCACAGT CCATGAAACC AGTGGAGAAA CATCTGCCTT TCCCGAAATT 
AGCATAGAAA CATCCACAGT CCATGAAATC AGTGGGGAAA GTTCTGCCTT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGGGGTGAAA CATCTGCGTT CCCTGAGATT 
ACCATAGAAG CCTCCACCAT TCAAGAGATC AGTGGAGAAA CATCTGCATT TCCTGAAATT 
AGCATAGCAA CTTCCACCGT CCGTGAAATC AGCGGCGAAA CGTCTGCCTT TCCTGAAATT 
AGAATAGAAA CATCCACGAG TCAAGAAGCC CGGGGTGAAA CATCTGCATT GCCTGAGATT 
ACCGTAGAGA CATCCACTGT CCATGAAACC AGTGGGGAAG CGTCTGCCTT TCCTGAAATT 
AGCATAGAAA CTTCCACAAG ACAAGAAGCC AGGGGTGAAG CATCCGCCTA CCCTGAGGTT 
AGCATAGAGG CATCCACAAC TCAAGAAGTA AGTGGGGAAA GTTCTGCCTT CCCTGAAATT 
AGCGTAGAGA CATCCACAAG TCAGGAAGCC CGCGGTGAAA CATCTGCCTT TCCTGAGATT 
GGCATAGAGA CATCCACAGC CCACGAAGGC AGTGGGGAAA CTCCTGGGCT GCCTGCTGTT 
AGCACTGACA CTGCTGCCAC GTCTCTGGCC AGTGGTGAGC CCTCCGGTGC TCCTGAGAAG 
GAAACTCCCG ACACAACATC ACATCTGATC ACGGGCGTTT CAGGGGAAAC CTCTGTCCCA 
GATGCTGTAA TCAGTACCAG TGCTCCAGAT GTTGAACTAG CGCAGGGACC CAGAAACACT 
GAAGAGACTC AGCTTGAAAT AGAGCCCTCC ACTCCTGCGG CATCTGGACA AGAGACAGAA 
ACAGCTGCTG TCCTCGACAA TCCCCATCTG CCAGCCACTG CTACTGCTGC CCTGCATCCA 
GCCTCCCAAG AAGCAGTAGA TGCACTGGGA CCCACGACAG AAGACCTGGC AAACTGTGAG 
GAAGGCTGGA TCAAGTTCCA GGGCCACTGC TACAGGCACT TTGAAGAGAG GGAGACGTGG 
ATGGACGCAG AGTCCAGGTG CAGAGAACAT CAAGCCCACC TGAGCAGCAT CATTACTCCA 
GAGGAACAAG AATTTGTGAA CAGCCATGCA CAAGACTATC AGTGGATCGG CCTCAGTGAC 
AGAGCCGTGG AGAATGATTT CCGCTGGTCT GATGGACACT CGCTGCAATT TGAGAACTGG 
CGGCCCAACC AACCTGACAA CTTCTTCTCC GCGGGCGAGG ACTGCGTTGT GATGATCTGG 
CATGAGCAAG GCGAATGGAA CGACGTCCCC TGCAACTATC ACCTGCCTTT CACGTGCAAG 
AAGGGAACAG TTGCCTGTGG GGACCCACCT GTAGTGGAGA ACGCGCGGAC CTTTGGCCGG 
AAGAAGGACC GCTATGAGAT CAACTCCCTG GTGCGGTACC AGTGTGACCA CGGCTACATC 
CAGCGCCACG TGCCCACCAT CCGCTGCCAA CCCAACGGGC ACTGGGAGGA ACCGCGGATA 
TCCTGCACAA ACCCCTCCAG CTACCAACGC CGGCTATACA AGAGGAGCCC CCGGAGCCGG 
TTGAGACCCG GTGTCGTGCA CAGACCCACC CATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hyaluronan binding to link module of TSG-6 and to G1 domain of aggrecan is differently regulated by pH.
The Journal of biological chemistry283(47)32294-301(2008 Nov)
Heng BC,Gribbon PM,Day AJ,Hardingham TE

Mutation analysis of the aggrecan gene in chickens with tibial dyschondroplasia.
Poultry science85(7)1169-72(2006 Jul)
Ray SA,Drummond PB,Shi L,McDaniel GR,Smith EJ

Differential versican isoforms and aggrecan expression in the chicken embryo aorta.
The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology279(1)592-600(2004 Jul)
Arciniegas E,Neves CY,Candelle D,Parada D

S103L reactive chondroitin sulfate proteoglycan (aggrecan) mRNA expressed in developing chick brain and cartilage is encoded by a single gene.
Brain research. Molecular brain research36(2)309-21(1996 Mar)
Li H,Domowicz M,Hennig A,Schwartz NB

Molecular cloning of chicken aggrecan.
The Biochemical journal296 ( Pt 3)885-7(1993 Dec)
Chandrasekaran L,Tanzer ML

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ACAN cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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