ROBO4 cDNA ORF clone, Gallus gallus(chicken)

The following ROBO4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ROBO4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa45874 XM_015298082.2
Latest version!
Gallus gallus roundabout guidance receptor 4 (ROBO4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OGa45875 XM_015298083.2
Latest version!
Gallus gallus roundabout guidance receptor 4 (ROBO4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OGa45876 XM_015298084.2
Latest version!
Gallus gallus roundabout guidance receptor 4 (ROBO4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
View Old Accession Versions >>
OGa45874 XM_015298082.1 Gallus gallus roundabout guidance receptor 4 (ROBO4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OGa45875 XM_015298083.1 Gallus gallus roundabout guidance receptor 4 (ROBO4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OGa45876 XM_015298084.1 Gallus gallus roundabout guidance receptor 4 (ROBO4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa45874
    Clone ID Related Accession (Same CDS sequence) XM_015298082.1 , XM_015298082.2
    Accession Version XM_015298082.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3195bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product roundabout homolog 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456007.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGGGCG GCTGGGCCAC GGCACTGGGG CTGGGGCTCT GGGTTACTGC ACTGCGCCTG 
    GGGGGCTGCC ACCCCTCCGG AGGGGACATG GAGTCACCCA CCACCCAGCG CCCAGCTGAG
    CTGCGGGACA ATTTCCGCCT GCAGCCCGGG GACCTGGTGG TTATGGCAGG GCAGGCGCTG
    GAGCTGGACT GCGTGCCCCC CGCCGGGCAG CCCGAGCCCC GCGTCACCTG GAGGAAGGAC
    GGCGTCGCCC TGCGCCTGGC TGGGGGCCGT CACCAGCTCA CACGGGGGAA GCTGTGGGTG
    GCCTCGGCGC GCAGGAGCGA CGCGGGGGTC TATGTCTGTG TGGCAGCCAA TGCAGCGGGC
    GAGCGGCACA GCCGGGGTGC CCACGTCACC ATCCTGGAGA AGCCGGTCAT CGTGCGGCGG
    CCAAGCGATG TCACAGCAGT GGCCGGCAGC ACAGTGGAGC TGAGCTGCGG TGCACAGGGC
    GACCCAGCAC CACGGGTTCA GTGGCATAAG GAGAGCGGGG ATCTGCCCTG GGGGAGGCAC
    GAGGTGGACC AGGAGCACAC ACTGCGACTC TACGCGGTGA CGGCAGCCGA TGGTGGTGCC
    TACGTCTGCA CAGCCCAGAG CCAGCTGGGC AGCACCGCTG CCACCGCCCG CCTCCGTGTG
    GAGGGGCAGC TGCCAGCAGG CTGGTGGGAG GCTACACGGC GGAACCTGCT GGCTGTGCAG
    CTGCAGCTGG ACAATGGCAC TGCACTGCCC ACCAATGCCA CTGTCTGGCT CCGCTGGCGG
    ATGCTGACAC CAGGGCCAGC TCCAGAGGGT TACGTGGTGC TGTATCGCTG CCTGCTCCCT
    GCCAGCACTG CCTGGTCCCA GCAGGATGTG GGCAGGGAGC TCAGCACCAT CATCCCTGCA
    CTCAAGCGGG GCTACCAGTA TGAGTTCAAA GTCCGACCCT ATGCCGGTGG GACCCAGGGC
    TTGGACAGCA ACAGCTGGCA CCTCTGGATC CCCGAAGAGG CAGTGCCAAG CGCGGCACCC
    CAGCGTGTCA CCGTGGGCCA AGCTGCACCG GGGAATGGTA CCGTCATCGT GAGCTGGGAG
    CCGCCTCCTC CTGATGCCCA CAATGGCATC ATTCGGGGCT ACCAGGTCTG GTCCCTGGGC
    GAGGGCTGGC TACAACCCAC CAACCATACA GTGGATGGAG GAACACGTCG CTTGGAGACC
    CTCCTGCATG GCCCTGGGGC CAGATACTGC ATCCAAGTAG CCGCGTTCAA TGGCGCAGGG
    CTGGGGATCC CCAGCAATGC CACCTGTGGT GTCCTGGAGA TGATGGCAGG GAGCACCGGA
    GTGGTGCAGG TGCTGCAGCA GCCTGTGGTC ATCGCAGCAA CTGGCTCACT GCTGTGGCTG
    GCCCTGCTTG CCCTCCTCCT CCTCATCTGC CAGCGTCGTG CCAGCCAGGA CTCCATGGCA
    CAACATGGGC AGGAGGACGG TGATGTGCCA TGGCTCAGCA GCCCCTGGAA ACCTGTCTGT
    GTCCCTCACA ACCTCAGTGG CAGCAGCAGC CTCAGCAGCC GGCTCCTGGG AAGCGACAGC
    AGGGACCCCC AGCCCTCTCC CCTGACCTCG GAGCTGCCAA GCCTCAGCCC TGCGACGACC
    CCCAGCTGCA GCCACCTCTG CAGAGAACAC CCCAAAACTA ACACCTCCAG CCTGGCACTG
    TGGGAGCACA TCCGCAAGCG AGAGCTGGAC CGTACGCACA GCTCCCCAGT GCCGGTGGCT
    GGGCTCAGGG CCATGCCCGT GCCCCTGTGT GGCGGGGAGT GGGGGACACA TTTCAAATCA
    GTGGACAGAT GGCCTCAGCA GAAAGGACAG GATGGTGACA CCGCAATAGC AGGAGAGGAC
    CCGTGGTGGC TGCCCACCAT CAGCTCCCCA AAACCACGGC GCAGTTCACT GGCATCAATT
    GGTGCTGGGA TGCTGGCGGT ACCTGCAGGA CATCCCCACG CCTGGCACCC CACGGGGACC
    CGGTTCCCGG TCATGGGGTG CTCAAGGGAC ACATCTCCAG TCACTGGGAA CCCCAGTGAT
    ATATCCACGG TCACCAGGAA CCCTGGGGAC ATGTCCCCAG TCACTAGGAA CACCAAGAAC
    ACACCTCTGA TCACCAGGAA CCACAGGGAC ATGTCCTCAG TCACGAGGAG CCCCGTAGAT
    ATGTCCCCTG CCACTGAGAA CCGCAGGGAC ATCTTACTGG CCACAAGACC TAGCAGAGAT
    GTGTCCCCGG TCACTGGGTG CCCCAAGGAC AGATCCCCGG TCATTGGGCA CTCCAAGGAA
    AAGTCCCCAG CCCCCAGGAG CCCCAGGGAC TTGTCCCCAG CCTCCAGTCA CTCCCTAGCC
    ACCACAGTTC AGGAGAACAC GTCCCCGGTC ACTCAGCACC CCAGGGACAT GTTCCCAGCC
    ACCAGGGGTC CCAGAGATGT GTTCCCAGCA GCCAGGCGCC CTGATGACAT GTCCATGGTC
    CCCCAGAACC CCAGGGACAC ATCTGTGCTC ACCAGACGAC CCAGGGACAT GCTGATGGCC
    ACCAAGGACA TGTCCCCAGG GCCCGGCCGC CTCTCACCAG CACTCAGTGA TGGGGTCCTC
    ACCCCGCAGC GGGTGGCCGA GGACCTGGAG AGGGTGCAGG AGGCCCTCCG CCCCACGAAC
    GCCCCCAGGC CAGAGGTGTC CCCAGCACGG CCCCAGCATG TCCCCGTGCC CCGGCCCCTG
    TCCCCTCCCC ACACCTACGG GTACATCTGC GGGCCTCCCG CCTCAGAGAT GGGTGAGGGA
    GAGGACGAGG AGGAGGTGGA AGAAGAGGAG GAGGAAGAGA AGTCCGAGGT GTGGGGTCAT
    GGGGGTTCGC CAGGGGGGTC TCTGCTGAAC GGATGGGGGT CTGTCTCGGA GGACAATGTC
    CCCAGCACCC GCTGCAGCCT GGCCAGCTCC TCCGATGGCT CCTTCCTGCT TGATGCCAGC
    TTTGCCCGTG CGCTGGCTGT GGCCGTGGAC AGCCTCTGCT TCAACCTCGA GGACAACGAT
    GGGGGCGGTG AGGGAACTGC TGCCCCCCTG CACCGCCTGG CATCATCCCC TCTGTGCGGG
    GAGGTGGCCC TGGGGACGGG GATGGGGATG GGGATGGGCG GCGTCTGGGC CAGGGTGGGC
    AGCCAGTGGG GTGGGCTCCT TTCCAAGGAC AGGAGGGACA GGAGTGTGGC AGCCCCTGGG
    TTATACCAGT GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015153568.1
    CDS335..3529
    Translation

    Target ORF information:

    RefSeq Version XM_015298082.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout guidance receptor 4 (ROBO4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298082.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGGGCG GCTGGGCCAC GGCACTGGGG CTGGGGCTCT GGGTTACTGC ACTGCGCCTG 
    GGGGGCTGCC ACCCCTCCGG AGGGGACATG GAGTCACCCA CCACCCAGCG CCCAGCTGAG
    CTGCGGGACA ATTTCCGCCT GCAGCCCGGG GACCTGGTGG TTATGGCAGG GCAGGCGCTG
    GAGCTGGACT GCGTGCCCCC CGCCGGGCAG CCCGAGCCCC GCGTCACCTG GAGGAAGGAC
    GGCGTCGCCC TGCGCCTGGC TGGGGGCCGT CACCAGCTCA CACGGGGGAA GCTGTGGGTG
    GCCTCGGCGC GCAGGAGCGA CGCGGGGGTC TATGTCTGTG TGGCAGCCAA TGCAGCGGGC
    GAGCGGCACA GCCGGGGTGC CCACGTCACC ATCCTGGAGA AGCCGGTCAT CGTGCGGCGG
    CCAAGCGATG TCACAGCAGT GGCCGGCAGC ACAGTGGAGC TGAGCTGCGG TGCACAGGGC
    GACCCAGCAC CACGGGTTCA GTGGCATAAG GAGAGCGGGG ATCTGCCCTG GGGGAGGCAC
    GAGGTGGACC AGGAGCACAC ACTGCGACTC TACGCGGTGA CGGCAGCCGA TGGTGGTGCC
    TACGTCTGCA CAGCCCAGAG CCAGCTGGGC AGCACCGCTG CCACCGCCCG CCTCCGTGTG
    GAGGGGCAGC TGCCAGCAGG CTGGTGGGAG GCTACACGGC GGAACCTGCT GGCTGTGCAG
    CTGCAGCTGG ACAATGGCAC TGCACTGCCC ACCAATGCCA CTGTCTGGCT CCGCTGGCGG
    ATGCTGACAC CAGGGCCAGC TCCAGAGGGT TACGTGGTGC TGTATCGCTG CCTGCTCCCT
    GCCAGCACTG CCTGGTCCCA GCAGGATGTG GGCAGGGAGC TCAGCACCAT CATCCCTGCA
    CTCAAGCGGG GCTACCAGTA TGAGTTCAAA GTCCGACCCT ATGCCGGTGG GACCCAGGGC
    TTGGACAGCA ACAGCTGGCA CCTCTGGATC CCCGAAGAGG CAGTGCCAAG CGCGGCACCC
    CAGCGTGTCA CCGTGGGCCA AGCTGCACCG GGGAATGGTA CCGTCATCGT GAGCTGGGAG
    CCGCCTCCTC CTGATGCCCA CAATGGCATC ATTCGGGGCT ACCAGGTCTG GTCCCTGGGC
    GAGGGCTGGC TACAACCCAC CAACCATACA GTGGATGGAG GAACACGTCG CTTGGAGACC
    CTCCTGCATG GCCCTGGGGC CAGATACTGC ATCCAAGTAG CCGCGTTCAA TGGCGCAGGG
    CTGGGGATCC CCAGCAATGC CACCTGTGGT GTCCTGGAGA TGATGGCAGG GAGCACCGGA
    GTGGTGCAGG TGCTGCAGCA GCCTGTGGTC ATCGCAGCAA CTGGCTCACT GCTGTGGCTG
    GCCCTGCTTG CCCTCCTCCT CCTCATCTGC CAGCGTCGTG CCAGCCAGGA CTCCATGGCA
    CAACATGGGC AGGAGGACGG TGATGTGCCA TGGCTCAGCA GCCCCTGGAA ACCTGTCTGT
    GTCCCTCACA ACCTCAGTGG CAGCAGCAGC CTCAGCAGCC GGCTCCTGGG AAGCGACAGC
    AGGGACCCCC AGCCCTCTCC CCTGACCTCG GAGCTGCCAA GCCTCAGCCC TGCGACGACC
    CCCAGCTGCA GCCACCTCTG CAGAGAACAC CCCAAAACTA ACACCTCCAG CCTGGCACTG
    TGGGAGCACA TCCGCAAGCG AGAGCTGGAC CGTACGCACA GCTCCCCAGT GCCGGTGGCT
    GGGCTCAGGG CCATGCCCGT GCCCCTGTGT GGCGGGGAGT GGGGGACACA TTTCAAATCA
    GTGGACAGAT GGCCTCAGCA GAAAGGACAG GATGGTGACA CCGCAATAGC AGGAGAGGAC
    CCGTGGTGGC TGCCCACCAT CAGCTCCCCA AAACCACGGC GCAGTTCACT GGCATCAATT
    GGTGCTGGGA TGCTGGCGGT ACCTGCAGGA CATCCCCACG CCTGGCACCC CACGGGGACC
    CGGTTCCCGG TCATGGGGTG CTCAAGGGAC ACATCTCCAG TCACTGGGAA CCCCAGTGAT
    ATATCCACGG TCACCAGGAA CCCTGGGGAC ATGTCCCCAG TCACTAGGAA CACCAAGAAC
    ACACCTCTGA TCACCAGGAA CCACAGGGAC ATGTCCTCAG TCACGAGGAG CCCCGTAGAT
    ATGTCCCCTG CCACTGAGAA CCGCAGGGAC ATCTTACTGG CCACAAGACC TAGCAGAGAT
    GTGTCCCCGG TCACTGGGTG CCCCAAGGAC AGATCCCCGG TCATTGGGCA CTCCAAGGAA
    AAGTCCCCAG CCCCCAGGAG CCCCAGGGAC TTGTCCCCAG CCTCCAGTCA CTCCCTAGCC
    ACCACAGTTC AGGAGAACAC GTCCCCGGTC ACTCAGCACC CCAGGGACAT GTTCCCAGCC
    ACCAGGGGTC CCAGAGATGT GTTCCCAGCA GCCAGGCGCC CTGATGACAT GTCCATGGTC
    CCCCAGAACC CCAGGGACAC ATCTGTGCTC ACCAGACGAC CCAGGGACAT GCTGATGGCC
    ACCAAGGACA TGTCCCCAGG GCCCGGCCGC CTCTCACCAG CACTCAGTGA TGGGGTCCTC
    ACCCCGCAGC GGGTGGCCGA GGACCTGGAG AGGGTGCAGG AGGCCCTCCG CCCCACGAAC
    GCCCCCAGGC CAGAGGTGTC CCCAGCACGG CCCCAGCATG TCCCCGTGCC CCGGCCCCTG
    TCCCCTCCCC ACACCTACGG GTACATCTGC GGGCCTCCCG CCTCAGAGAT GGGTGAGGGA
    GAGGACGAGG AGGAGGTGGA AGAAGAGGAG GAGGAAGAGA AGTCCGAGGT GTGGGGTCAT
    GGGGGTTCGC CAGGGGGGTC TCTGCTGAAC GGATGGGGGT CTGTCTCGGA GGACAATGTC
    CCCAGCACCC GCTGCAGCCT GGCCAGCTCC TCCGATGGCT CCTTCCTGCT TGATGCCAGC
    TTTGCCCGTG CGCTGGCTGT GGCCGTGGAC AGCCTCTGCT TCAACCTCGA GGACAACGAT
    GGGGGCGGTG AGGGAACTGC TGCCCCCCTG CACCGCCTGG CATCATCCCC TCTGTGCGGG
    GAGGTGGCCC TGGGGACGGG GATGGGGATG GGGATGGGCG GCGTCTGGGC CAGGGTGGGC
    AGCCAGTGGG GTGGGCTCCT TTCCAAGGAC AGGAGGGACA GGAGTGTGGC AGCCCCTGGG
    TTATACCAGT GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa45874
    Clone ID Related Accession (Same CDS sequence) XM_015298082.1 , XM_015298082.2
    Accession Version XM_015298082.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3195bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product roundabout homolog 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006111.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298082.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGGGCG GCTGGGCCAC GGCACTGGGG CTGGGGCTCT GGGTTACTGC ACTGCGCCTG 
    GGGGGCTGCC ACCCCTCCGG AGGGGACATG GAGTCACCCA CCACCCAGCG CCCAGCTGAG
    CTGCGGGACA ATTTCCGCCT GCAGCCCGGG GACCTGGTGG TTATGGCAGG GCAGGCGCTG
    GAGCTGGACT GCGTGCCCCC CGCCGGGCAG CCCGAGCCCC GCGTCACCTG GAGGAAGGAC
    GGCGTCGCCC TGCGCCTGGC TGGGGGCCGT CACCAGCTCA CACGGGGGAA GCTGTGGGTG
    GCCTCGGCGC GCAGGAGCGA CGCGGGGGTC TATGTCTGTG TGGCAGCCAA TGCAGCGGGC
    GAGCGGCACA GCCGGGGTGC CCACGTCACC ATCCTGGAGA AGCCGGTCAT CGTGCGGCGG
    CCAAGCGATG TCACAGCAGT GGCCGGCAGC ACAGTGGAGC TGAGCTGCGG TGCACAGGGC
    GACCCAGCAC CACGGGTTCA GTGGCATAAG GAGAGCGGGG ATCTGCCCTG GGGGAGGCAC
    GAGGTGGACC AGGAGCACAC ACTGCGACTC TACGCGGTGA CGGCAGCCGA TGGTGGTGCC
    TACGTCTGCA CAGCCCAGAG CCAGCTGGGC AGCACCGCTG CCACCGCCCG CCTCCGTGTG
    GAGGGGCAGC TGCCAGCAGG CTGGTGGGAG GCTACACGGC GGAACCTGCT GGCTGTGCAG
    CTGCAGCTGG ACAATGGCAC TGCACTGCCC ACCAATGCCA CTGTCTGGCT CCGCTGGCGG
    ATGCTGACAC CAGGGCCAGC TCCAGAGGGT TACGTGGTGC TGTATCGCTG CCTGCTCCCT
    GCCAGCACTG CCTGGTCCCA GCAGGATGTG GGCAGGGAGC TCAGCACCAT CATCCCTGCA
    CTCAAGCGGG GCTACCAGTA TGAGTTCAAA GTCCGACCCT ATGCCGGTGG GACCCAGGGC
    TTGGACAGCA ACAGCTGGCA CCTCTGGATC CCCGAAGAGG CAGTGCCAAG CGCGGCACCC
    CAGCGTGTCA CCGTGGGCCA AGCTGCACCG GGGAATGGTA CCGTCATCGT GAGCTGGGAG
    CCGCCTCCTC CTGATGCCCA CAATGGCATC ATTCGGGGCT ACCAGGTCTG GTCCCTGGGC
    GAGGGCTGGC TACAACCCAC CAACCATACA GTGGATGGAG GAACACGTCG CTTGGAGACC
    CTCCTGCATG GCCCTGGGGC CAGATACTGC ATCCAAGTAG CCGCGTTCAA TGGCGCAGGG
    CTGGGGATCC CCAGCAATGC CACCTGTGGT GTCCTGGAGA TGATGGCAGG GAGCACCGGA
    GTGGTGCAGG TGCTGCAGCA GCCTGTGGTC ATCGCAGCAA CTGGCTCACT GCTGTGGCTG
    GCCCTGCTTG CCCTCCTCCT CCTCATCTGC CAGCGTCGTG CCAGCCAGGA CTCCATGGCA
    CAACATGGGC AGGAGGACGG TGATGTGCCA TGGCTCAGCA GCCCCTGGAA ACCTGTCTGT
    GTCCCTCACA ACCTCAGTGG CAGCAGCAGC CTCAGCAGCC GGCTCCTGGG AAGCGACAGC
    AGGGACCCCC AGCCCTCTCC CCTGACCTCG GAGCTGCCAA GCCTCAGCCC TGCGACGACC
    CCCAGCTGCA GCCACCTCTG CAGAGAACAC CCCAAAACTA ACACCTCCAG CCTGGCACTG
    TGGGAGCACA TCCGCAAGCG AGAGCTGGAC CGTACGCACA GCTCCCCAGT GCCGGTGGCT
    GGGCTCAGGG CCATGCCCGT GCCCCTGTGT GGCGGGGAGT GGGGGACACA TTTCAAATCA
    GTGGACAGAT GGCCTCAGCA GAAAGGACAG GATGGTGACA CCGCAATAGC AGGAGAGGAC
    CCGTGGTGGC TGCCCACCAT CAGCTCCCCA AAACCACGGC GCAGTTCACT GGCATCAATT
    GGTGCTGGGA TGCTGGCGGT ACCTGCAGGA CATCCCCACG CCTGGCACCC CACGGGGACC
    CGGTTCCCGG TCATGGGGTG CTCAAGGGAC ACATCTCCAG TCACTGGGAA CCCCAGTGAT
    ATATCCACGG TCACCAGGAA CCCTGGGGAC ATGTCCCCAG TCACTAGGAA CACCAAGAAC
    ACACCTCTGA TCACCAGGAA CCACAGGGAC ATGTCCTCAG TCACGAGGAG CCCCGTAGAT
    ATGTCCCCTG CCACTGAGAA CCGCAGGGAC ATCTTACTGG CCACAAGACC TAGCAGAGAT
    GTGTCCCCGG TCACTGGGTG CCCCAAGGAC AGATCCCCGG TCATTGGGCA CTCCAAGGAA
    AAGTCCCCAG CCCCCAGGAG CCCCAGGGAC TTGTCCCCAG CCTCCAGTCA CTCCCTAGCC
    ACCACAGTTC AGGAGAACAC GTCCCCGGTC ACTCAGCACC CCAGGGACAT GTTCCCAGCC
    ACCAGGGGTC CCAGAGATGT GTTCCCAGCA GCCAGGCGCC CTGATGACAT GTCCATGGTC
    CCCCAGAACC CCAGGGACAC ATCTGTGCTC ACCAGACGAC CCAGGGACAT GCTGATGGCC
    ACCAAGGACA TGTCCCCAGG GCCCGGCCGC CTCTCACCAG CACTCAGTGA TGGGGTCCTC
    ACCCCGCAGC GGGTGGCCGA GGACCTGGAG AGGGTGCAGG AGGCCCTCCG CCCCACGAAC
    GCCCCCAGGC CAGAGGTGTC CCCAGCACGG CCCCAGCATG TCCCCGTGCC CCGGCCCCTG
    TCCCCTCCCC ACACCTACGG GTACATCTGC GGGCCTCCCG CCTCAGAGAT GGGTGAGGGA
    GAGGACGAGG AGGAGGTGGA AGAAGAGGAG GAGGAAGAGA AGTCCGAGGT GTGGGGTCAT
    GGGGGTTCGC CAGGGGGGTC TCTGCTGAAC GGATGGGGGT CTGTCTCGGA GGACAATGTC
    CCCAGCACCC GCTGCAGCCT GGCCAGCTCC TCCGATGGCT CCTTCCTGCT TGATGCCAGC
    TTTGCCCGTG CGCTGGCTGT GGCCGTGGAC AGCCTCTGCT TCAACCTCGA GGACAACGAT
    GGGGGCGGTG AGGGAACTGC TGCCCCCCTG CACCGCCTGG CATCATCCCC TCTGTGCGGG
    GAGGTGGCCC TGGGGACGGG GATGGGGATG GGGATGGGCG GCGTCTGGGC CAGGGTGGGC
    AGCCAGTGGG GTGGGCTCCT TTCCAAGGAC AGGAGGGACA GGAGTGTGGC AGCCCCTGGG
    TTATACCAGT GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015153568.1
    CDS299..3493
    Translation

    Target ORF information:

    RefSeq Version XM_015298082.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout guidance receptor 4 (ROBO4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298082.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGGGCG GCTGGGCCAC GGCACTGGGG CTGGGGCTCT GGGTTACTGC ACTGCGCCTG 
    GGGGGCTGCC ACCCCTCCGG AGGGGACATG GAGTCACCCA CCACCCAGCG CCCAGCTGAG
    CTGCGGGACA ATTTCCGCCT GCAGCCCGGG GACCTGGTGG TTATGGCAGG GCAGGCGCTG
    GAGCTGGACT GCGTGCCCCC CGCCGGGCAG CCCGAGCCCC GCGTCACCTG GAGGAAGGAC
    GGCGTCGCCC TGCGCCTGGC TGGGGGCCGT CACCAGCTCA CACGGGGGAA GCTGTGGGTG
    GCCTCGGCGC GCAGGAGCGA CGCGGGGGTC TATGTCTGTG TGGCAGCCAA TGCAGCGGGC
    GAGCGGCACA GCCGGGGTGC CCACGTCACC ATCCTGGAGA AGCCGGTCAT CGTGCGGCGG
    CCAAGCGATG TCACAGCAGT GGCCGGCAGC ACAGTGGAGC TGAGCTGCGG TGCACAGGGC
    GACCCAGCAC CACGGGTTCA GTGGCATAAG GAGAGCGGGG ATCTGCCCTG GGGGAGGCAC
    GAGGTGGACC AGGAGCACAC ACTGCGACTC TACGCGGTGA CGGCAGCCGA TGGTGGTGCC
    TACGTCTGCA CAGCCCAGAG CCAGCTGGGC AGCACCGCTG CCACCGCCCG CCTCCGTGTG
    GAGGGGCAGC TGCCAGCAGG CTGGTGGGAG GCTACACGGC GGAACCTGCT GGCTGTGCAG
    CTGCAGCTGG ACAATGGCAC TGCACTGCCC ACCAATGCCA CTGTCTGGCT CCGCTGGCGG
    ATGCTGACAC CAGGGCCAGC TCCAGAGGGT TACGTGGTGC TGTATCGCTG CCTGCTCCCT
    GCCAGCACTG CCTGGTCCCA GCAGGATGTG GGCAGGGAGC TCAGCACCAT CATCCCTGCA
    CTCAAGCGGG GCTACCAGTA TGAGTTCAAA GTCCGACCCT ATGCCGGTGG GACCCAGGGC
    TTGGACAGCA ACAGCTGGCA CCTCTGGATC CCCGAAGAGG CAGTGCCAAG CGCGGCACCC
    CAGCGTGTCA CCGTGGGCCA AGCTGCACCG GGGAATGGTA CCGTCATCGT GAGCTGGGAG
    CCGCCTCCTC CTGATGCCCA CAATGGCATC ATTCGGGGCT ACCAGGTCTG GTCCCTGGGC
    GAGGGCTGGC TACAACCCAC CAACCATACA GTGGATGGAG GAACACGTCG CTTGGAGACC
    CTCCTGCATG GCCCTGGGGC CAGATACTGC ATCCAAGTAG CCGCGTTCAA TGGCGCAGGG
    CTGGGGATCC CCAGCAATGC CACCTGTGGT GTCCTGGAGA TGATGGCAGG GAGCACCGGA
    GTGGTGCAGG TGCTGCAGCA GCCTGTGGTC ATCGCAGCAA CTGGCTCACT GCTGTGGCTG
    GCCCTGCTTG CCCTCCTCCT CCTCATCTGC CAGCGTCGTG CCAGCCAGGA CTCCATGGCA
    CAACATGGGC AGGAGGACGG TGATGTGCCA TGGCTCAGCA GCCCCTGGAA ACCTGTCTGT
    GTCCCTCACA ACCTCAGTGG CAGCAGCAGC CTCAGCAGCC GGCTCCTGGG AAGCGACAGC
    AGGGACCCCC AGCCCTCTCC CCTGACCTCG GAGCTGCCAA GCCTCAGCCC TGCGACGACC
    CCCAGCTGCA GCCACCTCTG CAGAGAACAC CCCAAAACTA ACACCTCCAG CCTGGCACTG
    TGGGAGCACA TCCGCAAGCG AGAGCTGGAC CGTACGCACA GCTCCCCAGT GCCGGTGGCT
    GGGCTCAGGG CCATGCCCGT GCCCCTGTGT GGCGGGGAGT GGGGGACACA TTTCAAATCA
    GTGGACAGAT GGCCTCAGCA GAAAGGACAG GATGGTGACA CCGCAATAGC AGGAGAGGAC
    CCGTGGTGGC TGCCCACCAT CAGCTCCCCA AAACCACGGC GCAGTTCACT GGCATCAATT
    GGTGCTGGGA TGCTGGCGGT ACCTGCAGGA CATCCCCACG CCTGGCACCC CACGGGGACC
    CGGTTCCCGG TCATGGGGTG CTCAAGGGAC ACATCTCCAG TCACTGGGAA CCCCAGTGAT
    ATATCCACGG TCACCAGGAA CCCTGGGGAC ATGTCCCCAG TCACTAGGAA CACCAAGAAC
    ACACCTCTGA TCACCAGGAA CCACAGGGAC ATGTCCTCAG TCACGAGGAG CCCCGTAGAT
    ATGTCCCCTG CCACTGAGAA CCGCAGGGAC ATCTTACTGG CCACAAGACC TAGCAGAGAT
    GTGTCCCCGG TCACTGGGTG CCCCAAGGAC AGATCCCCGG TCATTGGGCA CTCCAAGGAA
    AAGTCCCCAG CCCCCAGGAG CCCCAGGGAC TTGTCCCCAG CCTCCAGTCA CTCCCTAGCC
    ACCACAGTTC AGGAGAACAC GTCCCCGGTC ACTCAGCACC CCAGGGACAT GTTCCCAGCC
    ACCAGGGGTC CCAGAGATGT GTTCCCAGCA GCCAGGCGCC CTGATGACAT GTCCATGGTC
    CCCCAGAACC CCAGGGACAC ATCTGTGCTC ACCAGACGAC CCAGGGACAT GCTGATGGCC
    ACCAAGGACA TGTCCCCAGG GCCCGGCCGC CTCTCACCAG CACTCAGTGA TGGGGTCCTC
    ACCCCGCAGC GGGTGGCCGA GGACCTGGAG AGGGTGCAGG AGGCCCTCCG CCCCACGAAC
    GCCCCCAGGC CAGAGGTGTC CCCAGCACGG CCCCAGCATG TCCCCGTGCC CCGGCCCCTG
    TCCCCTCCCC ACACCTACGG GTACATCTGC GGGCCTCCCG CCTCAGAGAT GGGTGAGGGA
    GAGGACGAGG AGGAGGTGGA AGAAGAGGAG GAGGAAGAGA AGTCCGAGGT GTGGGGTCAT
    GGGGGTTCGC CAGGGGGGTC TCTGCTGAAC GGATGGGGGT CTGTCTCGGA GGACAATGTC
    CCCAGCACCC GCTGCAGCCT GGCCAGCTCC TCCGATGGCT CCTTCCTGCT TGATGCCAGC
    TTTGCCCGTG CGCTGGCTGT GGCCGTGGAC AGCCTCTGCT TCAACCTCGA GGACAACGAT
    GGGGGCGGTG AGGGAACTGC TGCCCCCCTG CACCGCCTGG CATCATCCCC TCTGTGCGGG
    GAGGTGGCCC TGGGGACGGG GATGGGGATG GGGATGGGCG GCGTCTGGGC CAGGGTGGGC
    AGCCAGTGGG GTGGGCTCCT TTCCAAGGAC AGGAGGGACA GGAGTGTGGC AGCCCCTGGG
    TTATACCAGT GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa45875
    Clone ID Related Accession (Same CDS sequence) XM_015298083.2 , XM_015298083.1
    Accession Version XM_015298083.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3192bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product roundabout homolog 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006111.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298083.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGGGCG GCTGGGCCAC GGCACTGGGG CTGGGGCTCT GGGTTACTGC ACTGCGCCTG 
    GGGGGCTGCC ACCCCTCCGG AGGGGACATG GAGTCACCCA CCACCCAGCG CCCAGCTGAG
    CTGCGGGACA ATTTCCGCCT GCAGCCCGGG GACCTGGTGG TTATGGCAGG GCAGGCGCTG
    GAGCTGGACT GCGTGCCCCC CGCCGGGCAG CCCGAGCCCC GCGTCACCTG GAGGAAGGAC
    GGCGTCGCCC TGCGCCTGGC TGGGGGCCGT CACCAGCTCA CACGGGGGAA GCTGTGGGTG
    GCCTCGGCGC GCAGGAGCGA CGCGGGGGTC TATGTCTGTG TGGCAGCCAA TGCAGCGGGC
    GAGCGGCACA GCCGGGGTGC CCACGTCACC ATCCTGGAGA AGCCGGTCAT CGTGCGGCGG
    CCAAGCGATG TCACAGCAGT GGCCGGCAGC ACAGTGGAGC TGAGCTGCGG TGCACAGGGC
    GACCCAGCAC CACGGGTTCA GTGGCATAAG GAGAGCGGGG ATCTGCCCTG GGGGAGGCAC
    GAGGTGGACC AGGAGCACAC ACTGCGACTC TACGCGGTGA CGGCAGCCGA TGGTGGTGCC
    TACGTCTGCA CAGCCCAGAG CCAGCTGGGC AGCACCGCTG CCACCGCCCG CCTCCGTGTG
    GAGGGGCAGC TGCCAGCAGG CTGGTGGGAG GCTACACGGC GGAACCTGCT GGCTGTGCAG
    CTGCAGCTGG ACAATGGCAC TGCACTGCCC ACCAATGCCA CTGTCTGGCT CCGCTGGCGG
    ATGCTGACAC CAGGGCCAGC TCCAGAGGGT TACGTGGTGC TGTATCGCTG CCTGCTCCCT
    GCCAGCACTG CCTGGTCCCA GCAGGATGTG GGCAGGGAGC TCAGCACCAT CATCCCTGCA
    CTCAAGCGGG GCTACCAGTA TGAGTTCAAA GTCCGACCCT ATGCCGGTGG GACCCAGGGC
    TTGGACAGCA ACAGCTGGCA CCTCTGGATC CCCGAAGAGG TGCCAAGCGC GGCACCCCAG
    CGTGTCACCG TGGGCCAAGC TGCACCGGGG AATGGTACCG TCATCGTGAG CTGGGAGCCG
    CCTCCTCCTG ATGCCCACAA TGGCATCATT CGGGGCTACC AGGTCTGGTC CCTGGGCGAG
    GGCTGGCTAC AACCCACCAA CCATACAGTG GATGGAGGAA CACGTCGCTT GGAGACCCTC
    CTGCATGGCC CTGGGGCCAG ATACTGCATC CAAGTAGCCG CGTTCAATGG CGCAGGGCTG
    GGGATCCCCA GCAATGCCAC CTGTGGTGTC CTGGAGATGA TGGCAGGGAG CACCGGAGTG
    GTGCAGGTGC TGCAGCAGCC TGTGGTCATC GCAGCAACTG GCTCACTGCT GTGGCTGGCC
    CTGCTTGCCC TCCTCCTCCT CATCTGCCAG CGTCGTGCCA GCCAGGACTC CATGGCACAA
    CATGGGCAGG AGGACGGTGA TGTGCCATGG CTCAGCAGCC CCTGGAAACC TGTCTGTGTC
    CCTCACAACC TCAGTGGCAG CAGCAGCCTC AGCAGCCGGC TCCTGGGAAG CGACAGCAGG
    GACCCCCAGC CCTCTCCCCT GACCTCGGAG CTGCCAAGCC TCAGCCCTGC GACGACCCCC
    AGCTGCAGCC ACCTCTGCAG AGAACACCCC AAAACTAACA CCTCCAGCCT GGCACTGTGG
    GAGCACATCC GCAAGCGAGA GCTGGACCGT ACGCACAGCT CCCCAGTGCC GGTGGCTGGG
    CTCAGGGCCA TGCCCGTGCC CCTGTGTGGC GGGGAGTGGG GGACACATTT CAAATCAGTG
    GACAGATGGC CTCAGCAGAA AGGACAGGAT GGTGACACCG CAATAGCAGG AGAGGACCCG
    TGGTGGCTGC CCACCATCAG CTCCCCAAAA CCACGGCGCA GTTCACTGGC ATCAATTGGT
    GCTGGGATGC TGGCGGTACC TGCAGGACAT CCCCACGCCT GGCACCCCAC GGGGACCCGG
    TTCCCGGTCA TGGGGTGCTC AAGGGACACA TCTCCAGTCA CTGGGAACCC CAGTGATATA
    TCCACGGTCA CCAGGAACCC TGGGGACATG TCCCCAGTCA CTAGGAACAC CAAGAACACA
    CCTCTGATCA CCAGGAACCA CAGGGACATG TCCTCAGTCA CGAGGAGCCC CGTAGATATG
    TCCCCTGCCA CTGAGAACCG CAGGGACATC TTACTGGCCA CAAGACCTAG CAGAGATGTG
    TCCCCGGTCA CTGGGTGCCC CAAGGACAGA TCCCCGGTCA TTGGGCACTC CAAGGAAAAG
    TCCCCAGCCC CCAGGAGCCC CAGGGACTTG TCCCCAGCCT CCAGTCACTC CCTAGCCACC
    ACAGTTCAGG AGAACACGTC CCCGGTCACT CAGCACCCCA GGGACATGTT CCCAGCCACC
    AGGGGTCCCA GAGATGTGTT CCCAGCAGCC AGGCGCCCTG ATGACATGTC CATGGTCCCC
    CAGAACCCCA GGGACACATC TGTGCTCACC AGACGACCCA GGGACATGCT GATGGCCACC
    AAGGACATGT CCCCAGGGCC CGGCCGCCTC TCACCAGCAC TCAGTGATGG GGTCCTCACC
    CCGCAGCGGG TGGCCGAGGA CCTGGAGAGG GTGCAGGAGG CCCTCCGCCC CACGAACGCC
    CCCAGGCCAG AGGTGTCCCC AGCACGGCCC CAGCATGTCC CCGTGCCCCG GCCCCTGTCC
    CCTCCCCACA CCTACGGGTA CATCTGCGGG CCTCCCGCCT CAGAGATGGG TGAGGGAGAG
    GACGAGGAGG AGGTGGAAGA AGAGGAGGAG GAAGAGAAGT CCGAGGTGTG GGGTCATGGG
    GGTTCGCCAG GGGGGTCTCT GCTGAACGGA TGGGGGTCTG TCTCGGAGGA CAATGTCCCC
    AGCACCCGCT GCAGCCTGGC CAGCTCCTCC GATGGCTCCT TCCTGCTTGA TGCCAGCTTT
    GCCCGTGCGC TGGCTGTGGC CGTGGACAGC CTCTGCTTCA ACCTCGAGGA CAACGATGGG
    GGCGGTGAGG GAACTGCTGC CCCCCTGCAC CGCCTGGCAT CATCCCCTCT GTGCGGGGAG
    GTGGCCCTGG GGACGGGGAT GGGGATGGGG ATGGGCGGCG TCTGGGCCAG GGTGGGCAGC
    CAGTGGGGTG GGCTCCTTTC CAAGGACAGG AGGGACAGGA GTGTGGCAGC CCCTGGGTTA
    TACCAGTGTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015153569.1
    CDS299..3490
    Translation

    Target ORF information:

    RefSeq Version XM_015298083.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout guidance receptor 4 (ROBO4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298083.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGGGCG GCTGGGCCAC GGCACTGGGG CTGGGGCTCT GGGTTACTGC ACTGCGCCTG 
    GGGGGCTGCC ACCCCTCCGG AGGGGACATG GAGTCACCCA CCACCCAGCG CCCAGCTGAG
    CTGCGGGACA ATTTCCGCCT GCAGCCCGGG GACCTGGTGG TTATGGCAGG GCAGGCGCTG
    GAGCTGGACT GCGTGCCCCC CGCCGGGCAG CCCGAGCCCC GCGTCACCTG GAGGAAGGAC
    GGCGTCGCCC TGCGCCTGGC TGGGGGCCGT CACCAGCTCA CACGGGGGAA GCTGTGGGTG
    GCCTCGGCGC GCAGGAGCGA CGCGGGGGTC TATGTCTGTG TGGCAGCCAA TGCAGCGGGC
    GAGCGGCACA GCCGGGGTGC CCACGTCACC ATCCTGGAGA AGCCGGTCAT CGTGCGGCGG
    CCAAGCGATG TCACAGCAGT GGCCGGCAGC ACAGTGGAGC TGAGCTGCGG TGCACAGGGC
    GACCCAGCAC CACGGGTTCA GTGGCATAAG GAGAGCGGGG ATCTGCCCTG GGGGAGGCAC
    GAGGTGGACC AGGAGCACAC ACTGCGACTC TACGCGGTGA CGGCAGCCGA TGGTGGTGCC
    TACGTCTGCA CAGCCCAGAG CCAGCTGGGC AGCACCGCTG CCACCGCCCG CCTCCGTGTG
    GAGGGGCAGC TGCCAGCAGG CTGGTGGGAG GCTACACGGC GGAACCTGCT GGCTGTGCAG
    CTGCAGCTGG ACAATGGCAC TGCACTGCCC ACCAATGCCA CTGTCTGGCT CCGCTGGCGG
    ATGCTGACAC CAGGGCCAGC TCCAGAGGGT TACGTGGTGC TGTATCGCTG CCTGCTCCCT
    GCCAGCACTG CCTGGTCCCA GCAGGATGTG GGCAGGGAGC TCAGCACCAT CATCCCTGCA
    CTCAAGCGGG GCTACCAGTA TGAGTTCAAA GTCCGACCCT ATGCCGGTGG GACCCAGGGC
    TTGGACAGCA ACAGCTGGCA CCTCTGGATC CCCGAAGAGG TGCCAAGCGC GGCACCCCAG
    CGTGTCACCG TGGGCCAAGC TGCACCGGGG AATGGTACCG TCATCGTGAG CTGGGAGCCG
    CCTCCTCCTG ATGCCCACAA TGGCATCATT CGGGGCTACC AGGTCTGGTC CCTGGGCGAG
    GGCTGGCTAC AACCCACCAA CCATACAGTG GATGGAGGAA CACGTCGCTT GGAGACCCTC
    CTGCATGGCC CTGGGGCCAG ATACTGCATC CAAGTAGCCG CGTTCAATGG CGCAGGGCTG
    GGGATCCCCA GCAATGCCAC CTGTGGTGTC CTGGAGATGA TGGCAGGGAG CACCGGAGTG
    GTGCAGGTGC TGCAGCAGCC TGTGGTCATC GCAGCAACTG GCTCACTGCT GTGGCTGGCC
    CTGCTTGCCC TCCTCCTCCT CATCTGCCAG CGTCGTGCCA GCCAGGACTC CATGGCACAA
    CATGGGCAGG AGGACGGTGA TGTGCCATGG CTCAGCAGCC CCTGGAAACC TGTCTGTGTC
    CCTCACAACC TCAGTGGCAG CAGCAGCCTC AGCAGCCGGC TCCTGGGAAG CGACAGCAGG
    GACCCCCAGC CCTCTCCCCT GACCTCGGAG CTGCCAAGCC TCAGCCCTGC GACGACCCCC
    AGCTGCAGCC ACCTCTGCAG AGAACACCCC AAAACTAACA CCTCCAGCCT GGCACTGTGG
    GAGCACATCC GCAAGCGAGA GCTGGACCGT ACGCACAGCT CCCCAGTGCC GGTGGCTGGG
    CTCAGGGCCA TGCCCGTGCC CCTGTGTGGC GGGGAGTGGG GGACACATTT CAAATCAGTG
    GACAGATGGC CTCAGCAGAA AGGACAGGAT GGTGACACCG CAATAGCAGG AGAGGACCCG
    TGGTGGCTGC CCACCATCAG CTCCCCAAAA CCACGGCGCA GTTCACTGGC ATCAATTGGT
    GCTGGGATGC TGGCGGTACC TGCAGGACAT CCCCACGCCT GGCACCCCAC GGGGACCCGG
    TTCCCGGTCA TGGGGTGCTC AAGGGACACA TCTCCAGTCA CTGGGAACCC CAGTGATATA
    TCCACGGTCA CCAGGAACCC TGGGGACATG TCCCCAGTCA CTAGGAACAC CAAGAACACA
    CCTCTGATCA CCAGGAACCA CAGGGACATG TCCTCAGTCA CGAGGAGCCC CGTAGATATG
    TCCCCTGCCA CTGAGAACCG CAGGGACATC TTACTGGCCA CAAGACCTAG CAGAGATGTG
    TCCCCGGTCA CTGGGTGCCC CAAGGACAGA TCCCCGGTCA TTGGGCACTC CAAGGAAAAG
    TCCCCAGCCC CCAGGAGCCC CAGGGACTTG TCCCCAGCCT CCAGTCACTC CCTAGCCACC
    ACAGTTCAGG AGAACACGTC CCCGGTCACT CAGCACCCCA GGGACATGTT CCCAGCCACC
    AGGGGTCCCA GAGATGTGTT CCCAGCAGCC AGGCGCCCTG ATGACATGTC CATGGTCCCC
    CAGAACCCCA GGGACACATC TGTGCTCACC AGACGACCCA GGGACATGCT GATGGCCACC
    AAGGACATGT CCCCAGGGCC CGGCCGCCTC TCACCAGCAC TCAGTGATGG GGTCCTCACC
    CCGCAGCGGG TGGCCGAGGA CCTGGAGAGG GTGCAGGAGG CCCTCCGCCC CACGAACGCC
    CCCAGGCCAG AGGTGTCCCC AGCACGGCCC CAGCATGTCC CCGTGCCCCG GCCCCTGTCC
    CCTCCCCACA CCTACGGGTA CATCTGCGGG CCTCCCGCCT CAGAGATGGG TGAGGGAGAG
    GACGAGGAGG AGGTGGAAGA AGAGGAGGAG GAAGAGAAGT CCGAGGTGTG GGGTCATGGG
    GGTTCGCCAG GGGGGTCTCT GCTGAACGGA TGGGGGTCTG TCTCGGAGGA CAATGTCCCC
    AGCACCCGCT GCAGCCTGGC CAGCTCCTCC GATGGCTCCT TCCTGCTTGA TGCCAGCTTT
    GCCCGTGCGC TGGCTGTGGC CGTGGACAGC CTCTGCTTCA ACCTCGAGGA CAACGATGGG
    GGCGGTGAGG GAACTGCTGC CCCCCTGCAC CGCCTGGCAT CATCCCCTCT GTGCGGGGAG
    GTGGCCCTGG GGACGGGGAT GGGGATGGGG ATGGGCGGCG TCTGGGCCAG GGTGGGCAGC
    CAGTGGGGTG GGCTCCTTTC CAAGGACAGG AGGGACAGGA GTGTGGCAGC CCCTGGGTTA
    TACCAGTGTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa45875
    Clone ID Related Accession (Same CDS sequence) XM_015298083.2 , XM_015298083.1
    Accession Version XM_015298083.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3192bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product roundabout homolog 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456007.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGGGCG GCTGGGCCAC GGCACTGGGG CTGGGGCTCT GGGTTACTGC ACTGCGCCTG 
    GGGGGCTGCC ACCCCTCCGG AGGGGACATG GAGTCACCCA CCACCCAGCG CCCAGCTGAG
    CTGCGGGACA ATTTCCGCCT GCAGCCCGGG GACCTGGTGG TTATGGCAGG GCAGGCGCTG
    GAGCTGGACT GCGTGCCCCC CGCCGGGCAG CCCGAGCCCC GCGTCACCTG GAGGAAGGAC
    GGCGTCGCCC TGCGCCTGGC TGGGGGCCGT CACCAGCTCA CACGGGGGAA GCTGTGGGTG
    GCCTCGGCGC GCAGGAGCGA CGCGGGGGTC TATGTCTGTG TGGCAGCCAA TGCAGCGGGC
    GAGCGGCACA GCCGGGGTGC CCACGTCACC ATCCTGGAGA AGCCGGTCAT CGTGCGGCGG
    CCAAGCGATG TCACAGCAGT GGCCGGCAGC ACAGTGGAGC TGAGCTGCGG TGCACAGGGC
    GACCCAGCAC CACGGGTTCA GTGGCATAAG GAGAGCGGGG ATCTGCCCTG GGGGAGGCAC
    GAGGTGGACC AGGAGCACAC ACTGCGACTC TACGCGGTGA CGGCAGCCGA TGGTGGTGCC
    TACGTCTGCA CAGCCCAGAG CCAGCTGGGC AGCACCGCTG CCACCGCCCG CCTCCGTGTG
    GAGGGGCAGC TGCCAGCAGG CTGGTGGGAG GCTACACGGC GGAACCTGCT GGCTGTGCAG
    CTGCAGCTGG ACAATGGCAC TGCACTGCCC ACCAATGCCA CTGTCTGGCT CCGCTGGCGG
    ATGCTGACAC CAGGGCCAGC TCCAGAGGGT TACGTGGTGC TGTATCGCTG CCTGCTCCCT
    GCCAGCACTG CCTGGTCCCA GCAGGATGTG GGCAGGGAGC TCAGCACCAT CATCCCTGCA
    CTCAAGCGGG GCTACCAGTA TGAGTTCAAA GTCCGACCCT ATGCCGGTGG GACCCAGGGC
    TTGGACAGCA ACAGCTGGCA CCTCTGGATC CCCGAAGAGG TGCCAAGCGC GGCACCCCAG
    CGTGTCACCG TGGGCCAAGC TGCACCGGGG AATGGTACCG TCATCGTGAG CTGGGAGCCG
    CCTCCTCCTG ATGCCCACAA TGGCATCATT CGGGGCTACC AGGTCTGGTC CCTGGGCGAG
    GGCTGGCTAC AACCCACCAA CCATACAGTG GATGGAGGAA CACGTCGCTT GGAGACCCTC
    CTGCATGGCC CTGGGGCCAG ATACTGCATC CAAGTAGCCG CGTTCAATGG CGCAGGGCTG
    GGGATCCCCA GCAATGCCAC CTGTGGTGTC CTGGAGATGA TGGCAGGGAG CACCGGAGTG
    GTGCAGGTGC TGCAGCAGCC TGTGGTCATC GCAGCAACTG GCTCACTGCT GTGGCTGGCC
    CTGCTTGCCC TCCTCCTCCT CATCTGCCAG CGTCGTGCCA GCCAGGACTC CATGGCACAA
    CATGGGCAGG AGGACGGTGA TGTGCCATGG CTCAGCAGCC CCTGGAAACC TGTCTGTGTC
    CCTCACAACC TCAGTGGCAG CAGCAGCCTC AGCAGCCGGC TCCTGGGAAG CGACAGCAGG
    GACCCCCAGC CCTCTCCCCT GACCTCGGAG CTGCCAAGCC TCAGCCCTGC GACGACCCCC
    AGCTGCAGCC ACCTCTGCAG AGAACACCCC AAAACTAACA CCTCCAGCCT GGCACTGTGG
    GAGCACATCC GCAAGCGAGA GCTGGACCGT ACGCACAGCT CCCCAGTGCC GGTGGCTGGG
    CTCAGGGCCA TGCCCGTGCC CCTGTGTGGC GGGGAGTGGG GGACACATTT CAAATCAGTG
    GACAGATGGC CTCAGCAGAA AGGACAGGAT GGTGACACCG CAATAGCAGG AGAGGACCCG
    TGGTGGCTGC CCACCATCAG CTCCCCAAAA CCACGGCGCA GTTCACTGGC ATCAATTGGT
    GCTGGGATGC TGGCGGTACC TGCAGGACAT CCCCACGCCT GGCACCCCAC GGGGACCCGG
    TTCCCGGTCA TGGGGTGCTC AAGGGACACA TCTCCAGTCA CTGGGAACCC CAGTGATATA
    TCCACGGTCA CCAGGAACCC TGGGGACATG TCCCCAGTCA CTAGGAACAC CAAGAACACA
    CCTCTGATCA CCAGGAACCA CAGGGACATG TCCTCAGTCA CGAGGAGCCC CGTAGATATG
    TCCCCTGCCA CTGAGAACCG CAGGGACATC TTACTGGCCA CAAGACCTAG CAGAGATGTG
    TCCCCGGTCA CTGGGTGCCC CAAGGACAGA TCCCCGGTCA TTGGGCACTC CAAGGAAAAG
    TCCCCAGCCC CCAGGAGCCC CAGGGACTTG TCCCCAGCCT CCAGTCACTC CCTAGCCACC
    ACAGTTCAGG AGAACACGTC CCCGGTCACT CAGCACCCCA GGGACATGTT CCCAGCCACC
    AGGGGTCCCA GAGATGTGTT CCCAGCAGCC AGGCGCCCTG ATGACATGTC CATGGTCCCC
    CAGAACCCCA GGGACACATC TGTGCTCACC AGACGACCCA GGGACATGCT GATGGCCACC
    AAGGACATGT CCCCAGGGCC CGGCCGCCTC TCACCAGCAC TCAGTGATGG GGTCCTCACC
    CCGCAGCGGG TGGCCGAGGA CCTGGAGAGG GTGCAGGAGG CCCTCCGCCC CACGAACGCC
    CCCAGGCCAG AGGTGTCCCC AGCACGGCCC CAGCATGTCC CCGTGCCCCG GCCCCTGTCC
    CCTCCCCACA CCTACGGGTA CATCTGCGGG CCTCCCGCCT CAGAGATGGG TGAGGGAGAG
    GACGAGGAGG AGGTGGAAGA AGAGGAGGAG GAAGAGAAGT CCGAGGTGTG GGGTCATGGG
    GGTTCGCCAG GGGGGTCTCT GCTGAACGGA TGGGGGTCTG TCTCGGAGGA CAATGTCCCC
    AGCACCCGCT GCAGCCTGGC CAGCTCCTCC GATGGCTCCT TCCTGCTTGA TGCCAGCTTT
    GCCCGTGCGC TGGCTGTGGC CGTGGACAGC CTCTGCTTCA ACCTCGAGGA CAACGATGGG
    GGCGGTGAGG GAACTGCTGC CCCCCTGCAC CGCCTGGCAT CATCCCCTCT GTGCGGGGAG
    GTGGCCCTGG GGACGGGGAT GGGGATGGGG ATGGGCGGCG TCTGGGCCAG GGTGGGCAGC
    CAGTGGGGTG GGCTCCTTTC CAAGGACAGG AGGGACAGGA GTGTGGCAGC CCCTGGGTTA
    TACCAGTGTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015153569.1
    CDS335..3526
    Translation

    Target ORF information:

    RefSeq Version XM_015298083.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout guidance receptor 4 (ROBO4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298083.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGGGCG GCTGGGCCAC GGCACTGGGG CTGGGGCTCT GGGTTACTGC ACTGCGCCTG 
    GGGGGCTGCC ACCCCTCCGG AGGGGACATG GAGTCACCCA CCACCCAGCG CCCAGCTGAG
    CTGCGGGACA ATTTCCGCCT GCAGCCCGGG GACCTGGTGG TTATGGCAGG GCAGGCGCTG
    GAGCTGGACT GCGTGCCCCC CGCCGGGCAG CCCGAGCCCC GCGTCACCTG GAGGAAGGAC
    GGCGTCGCCC TGCGCCTGGC TGGGGGCCGT CACCAGCTCA CACGGGGGAA GCTGTGGGTG
    GCCTCGGCGC GCAGGAGCGA CGCGGGGGTC TATGTCTGTG TGGCAGCCAA TGCAGCGGGC
    GAGCGGCACA GCCGGGGTGC CCACGTCACC ATCCTGGAGA AGCCGGTCAT CGTGCGGCGG
    CCAAGCGATG TCACAGCAGT GGCCGGCAGC ACAGTGGAGC TGAGCTGCGG TGCACAGGGC
    GACCCAGCAC CACGGGTTCA GTGGCATAAG GAGAGCGGGG ATCTGCCCTG GGGGAGGCAC
    GAGGTGGACC AGGAGCACAC ACTGCGACTC TACGCGGTGA CGGCAGCCGA TGGTGGTGCC
    TACGTCTGCA CAGCCCAGAG CCAGCTGGGC AGCACCGCTG CCACCGCCCG CCTCCGTGTG
    GAGGGGCAGC TGCCAGCAGG CTGGTGGGAG GCTACACGGC GGAACCTGCT GGCTGTGCAG
    CTGCAGCTGG ACAATGGCAC TGCACTGCCC ACCAATGCCA CTGTCTGGCT CCGCTGGCGG
    ATGCTGACAC CAGGGCCAGC TCCAGAGGGT TACGTGGTGC TGTATCGCTG CCTGCTCCCT
    GCCAGCACTG CCTGGTCCCA GCAGGATGTG GGCAGGGAGC TCAGCACCAT CATCCCTGCA
    CTCAAGCGGG GCTACCAGTA TGAGTTCAAA GTCCGACCCT ATGCCGGTGG GACCCAGGGC
    TTGGACAGCA ACAGCTGGCA CCTCTGGATC CCCGAAGAGG TGCCAAGCGC GGCACCCCAG
    CGTGTCACCG TGGGCCAAGC TGCACCGGGG AATGGTACCG TCATCGTGAG CTGGGAGCCG
    CCTCCTCCTG ATGCCCACAA TGGCATCATT CGGGGCTACC AGGTCTGGTC CCTGGGCGAG
    GGCTGGCTAC AACCCACCAA CCATACAGTG GATGGAGGAA CACGTCGCTT GGAGACCCTC
    CTGCATGGCC CTGGGGCCAG ATACTGCATC CAAGTAGCCG CGTTCAATGG CGCAGGGCTG
    GGGATCCCCA GCAATGCCAC CTGTGGTGTC CTGGAGATGA TGGCAGGGAG CACCGGAGTG
    GTGCAGGTGC TGCAGCAGCC TGTGGTCATC GCAGCAACTG GCTCACTGCT GTGGCTGGCC
    CTGCTTGCCC TCCTCCTCCT CATCTGCCAG CGTCGTGCCA GCCAGGACTC CATGGCACAA
    CATGGGCAGG AGGACGGTGA TGTGCCATGG CTCAGCAGCC CCTGGAAACC TGTCTGTGTC
    CCTCACAACC TCAGTGGCAG CAGCAGCCTC AGCAGCCGGC TCCTGGGAAG CGACAGCAGG
    GACCCCCAGC CCTCTCCCCT GACCTCGGAG CTGCCAAGCC TCAGCCCTGC GACGACCCCC
    AGCTGCAGCC ACCTCTGCAG AGAACACCCC AAAACTAACA CCTCCAGCCT GGCACTGTGG
    GAGCACATCC GCAAGCGAGA GCTGGACCGT ACGCACAGCT CCCCAGTGCC GGTGGCTGGG
    CTCAGGGCCA TGCCCGTGCC CCTGTGTGGC GGGGAGTGGG GGACACATTT CAAATCAGTG
    GACAGATGGC CTCAGCAGAA AGGACAGGAT GGTGACACCG CAATAGCAGG AGAGGACCCG
    TGGTGGCTGC CCACCATCAG CTCCCCAAAA CCACGGCGCA GTTCACTGGC ATCAATTGGT
    GCTGGGATGC TGGCGGTACC TGCAGGACAT CCCCACGCCT GGCACCCCAC GGGGACCCGG
    TTCCCGGTCA TGGGGTGCTC AAGGGACACA TCTCCAGTCA CTGGGAACCC CAGTGATATA
    TCCACGGTCA CCAGGAACCC TGGGGACATG TCCCCAGTCA CTAGGAACAC CAAGAACACA
    CCTCTGATCA CCAGGAACCA CAGGGACATG TCCTCAGTCA CGAGGAGCCC CGTAGATATG
    TCCCCTGCCA CTGAGAACCG CAGGGACATC TTACTGGCCA CAAGACCTAG CAGAGATGTG
    TCCCCGGTCA CTGGGTGCCC CAAGGACAGA TCCCCGGTCA TTGGGCACTC CAAGGAAAAG
    TCCCCAGCCC CCAGGAGCCC CAGGGACTTG TCCCCAGCCT CCAGTCACTC CCTAGCCACC
    ACAGTTCAGG AGAACACGTC CCCGGTCACT CAGCACCCCA GGGACATGTT CCCAGCCACC
    AGGGGTCCCA GAGATGTGTT CCCAGCAGCC AGGCGCCCTG ATGACATGTC CATGGTCCCC
    CAGAACCCCA GGGACACATC TGTGCTCACC AGACGACCCA GGGACATGCT GATGGCCACC
    AAGGACATGT CCCCAGGGCC CGGCCGCCTC TCACCAGCAC TCAGTGATGG GGTCCTCACC
    CCGCAGCGGG TGGCCGAGGA CCTGGAGAGG GTGCAGGAGG CCCTCCGCCC CACGAACGCC
    CCCAGGCCAG AGGTGTCCCC AGCACGGCCC CAGCATGTCC CCGTGCCCCG GCCCCTGTCC
    CCTCCCCACA CCTACGGGTA CATCTGCGGG CCTCCCGCCT CAGAGATGGG TGAGGGAGAG
    GACGAGGAGG AGGTGGAAGA AGAGGAGGAG GAAGAGAAGT CCGAGGTGTG GGGTCATGGG
    GGTTCGCCAG GGGGGTCTCT GCTGAACGGA TGGGGGTCTG TCTCGGAGGA CAATGTCCCC
    AGCACCCGCT GCAGCCTGGC CAGCTCCTCC GATGGCTCCT TCCTGCTTGA TGCCAGCTTT
    GCCCGTGCGC TGGCTGTGGC CGTGGACAGC CTCTGCTTCA ACCTCGAGGA CAACGATGGG
    GGCGGTGAGG GAACTGCTGC CCCCCTGCAC CGCCTGGCAT CATCCCCTCT GTGCGGGGAG
    GTGGCCCTGG GGACGGGGAT GGGGATGGGG ATGGGCGGCG TCTGGGCCAG GGTGGGCAGC
    CAGTGGGGTG GGCTCCTTTC CAAGGACAGG AGGGACAGGA GTGTGGCAGC CCCTGGGTTA
    TACCAGTGTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa45876
    Clone ID Related Accession (Same CDS sequence) XM_015298084.2 , XM_015298084.1
    Accession Version XM_015298084.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3144bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product roundabout homolog 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006111.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298084.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCGGGCG GCTGGGCCAC GGCACTGGGG CTGGGGCTCT GGGTTACTGC ACTGCGCCTG 
    GGGGGCTGCC ACCCCTCCGG AGGGGACATG GAGTCACCCA CCACCCAGCG CCCAGCTGGG
    CAGGCGCTGG AGCTGGACTG CGTGCCCCCC GCCGGGCAGC CCGAGCCCCG CGTCACCTGG
    AGGAAGGACG GCGTCGCCCT GCGCCTGGCT GGGGGCCGTC ACCAGCTCAC ACGGGGGAAG
    CTGTGGGTGG CCTCGGCGCG CAGGAGCGAC GCGGGGGTCT ATGTCTGTGT GGCAGCCAAT
    GCAGCGGGCG AGCGGCACAG CCGGGGTGCC CACGTCACCA TCCTGGAGAA GCCGGTCATC
    GTGCGGCGGC CAAGCGATGT CACAGCAGTG GCCGGCAGCA CAGTGGAGCT GAGCTGCGGT
    GCACAGGGCG ACCCAGCACC ACGGGTTCAG TGGCATAAGG AGAGCGGGGA TCTGCCCTGG
    GGGAGGCACG AGGTGGACCA GGAGCACACA CTGCGACTCT ACGCGGTGAC GGCAGCCGAT
    GGTGGTGCCT ACGTCTGCAC AGCCCAGAGC CAGCTGGGCA GCACCGCTGC CACCGCCCGC
    CTCCGTGTGG AGGGGCAGCT GCCAGCAGGC TGGTGGGAGG CTACACGGCG GAACCTGCTG
    GCTGTGCAGC TGCAGCTGGA CAATGGCACT GCACTGCCCA CCAATGCCAC TGTCTGGCTC
    CGCTGGCGGA TGCTGACACC AGGGCCAGCT CCAGAGGGTT ACGTGGTGCT GTATCGCTGC
    CTGCTCCCTG CCAGCACTGC CTGGTCCCAG CAGGATGTGG GCAGGGAGCT CAGCACCATC
    ATCCCTGCAC TCAAGCGGGG CTACCAGTAT GAGTTCAAAG TCCGACCCTA TGCCGGTGGG
    ACCCAGGGCT TGGACAGCAA CAGCTGGCAC CTCTGGATCC CCGAAGAGGC AGTGCCAAGC
    GCGGCACCCC AGCGTGTCAC CGTGGGCCAA GCTGCACCGG GGAATGGTAC CGTCATCGTG
    AGCTGGGAGC CGCCTCCTCC TGATGCCCAC AATGGCATCA TTCGGGGCTA CCAGGTCTGG
    TCCCTGGGCG AGGGCTGGCT ACAACCCACC AACCATACAG TGGATGGAGG AACACGTCGC
    TTGGAGACCC TCCTGCATGG CCCTGGGGCC AGATACTGCA TCCAAGTAGC CGCGTTCAAT
    GGCGCAGGGC TGGGGATCCC CAGCAATGCC ACCTGTGGTG TCCTGGAGAT GATGGCAGGG
    AGCACCGGAG TGGTGCAGGT GCTGCAGCAG CCTGTGGTCA TCGCAGCAAC TGGCTCACTG
    CTGTGGCTGG CCCTGCTTGC CCTCCTCCTC CTCATCTGCC AGCGTCGTGC CAGCCAGGAC
    TCCATGGCAC AACATGGGCA GGAGGACGGT GATGTGCCAT GGCTCAGCAG CCCCTGGAAA
    CCTGTCTGTG TCCCTCACAA CCTCAGTGGC AGCAGCAGCC TCAGCAGCCG GCTCCTGGGA
    AGCGACAGCA GGGACCCCCA GCCCTCTCCC CTGACCTCGG AGCTGCCAAG CCTCAGCCCT
    GCGACGACCC CCAGCTGCAG CCACCTCTGC AGAGAACACC CCAAAACTAA CACCTCCAGC
    CTGGCACTGT GGGAGCACAT CCGCAAGCGA GAGCTGGACC GTACGCACAG CTCCCCAGTG
    CCGGTGGCTG GGCTCAGGGC CATGCCCGTG CCCCTGTGTG GCGGGGAGTG GGGGACACAT
    TTCAAATCAG TGGACAGATG GCCTCAGCAG AAAGGACAGG ATGGTGACAC CGCAATAGCA
    GGAGAGGACC CGTGGTGGCT GCCCACCATC AGCTCCCCAA AACCACGGCG CAGTTCACTG
    GCATCAATTG GTGCTGGGAT GCTGGCGGTA CCTGCAGGAC ATCCCCACGC CTGGCACCCC
    ACGGGGACCC GGTTCCCGGT CATGGGGTGC TCAAGGGACA CATCTCCAGT CACTGGGAAC
    CCCAGTGATA TATCCACGGT CACCAGGAAC CCTGGGGACA TGTCCCCAGT CACTAGGAAC
    ACCAAGAACA CACCTCTGAT CACCAGGAAC CACAGGGACA TGTCCTCAGT CACGAGGAGC
    CCCGTAGATA TGTCCCCTGC CACTGAGAAC CGCAGGGACA TCTTACTGGC CACAAGACCT
    AGCAGAGATG TGTCCCCGGT CACTGGGTGC CCCAAGGACA GATCCCCGGT CATTGGGCAC
    TCCAAGGAAA AGTCCCCAGC CCCCAGGAGC CCCAGGGACT TGTCCCCAGC CTCCAGTCAC
    TCCCTAGCCA CCACAGTTCA GGAGAACACG TCCCCGGTCA CTCAGCACCC CAGGGACATG
    TTCCCAGCCA CCAGGGGTCC CAGAGATGTG TTCCCAGCAG CCAGGCGCCC TGATGACATG
    TCCATGGTCC CCCAGAACCC CAGGGACACA TCTGTGCTCA CCAGACGACC CAGGGACATG
    CTGATGGCCA CCAAGGACAT GTCCCCAGGG CCCGGCCGCC TCTCACCAGC ACTCAGTGAT
    GGGGTCCTCA CCCCGCAGCG GGTGGCCGAG GACCTGGAGA GGGTGCAGGA GGCCCTCCGC
    CCCACGAACG CCCCCAGGCC AGAGGTGTCC CCAGCACGGC CCCAGCATGT CCCCGTGCCC
    CGGCCCCTGT CCCCTCCCCA CACCTACGGG TACATCTGCG GGCCTCCCGC CTCAGAGATG
    GGTGAGGGAG AGGACGAGGA GGAGGTGGAA GAAGAGGAGG AGGAAGAGAA GTCCGAGGTG
    TGGGGTCATG GGGGTTCGCC AGGGGGGTCT CTGCTGAACG GATGGGGGTC TGTCTCGGAG
    GACAATGTCC CCAGCACCCG CTGCAGCCTG GCCAGCTCCT CCGATGGCTC CTTCCTGCTT
    GATGCCAGCT TTGCCCGTGC GCTGGCTGTG GCCGTGGACA GCCTCTGCTT CAACCTCGAG
    GACAACGATG GGGGCGGTGA GGGAACTGCT GCCCCCCTGC ACCGCCTGGC ATCATCCCCT
    CTGTGCGGGG AGGTGGCCCT GGGGACGGGG ATGGGGATGG GGATGGGCGG CGTCTGGGCC
    AGGGTGGGCA GCCAGTGGGG TGGGCTCCTT TCCAAGGACA GGAGGGACAG GAGTGTGGCA
    GCCCCTGGGT TATACCAGTG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015153570.1
    CDS299..3442
    Translation

    Target ORF information:

    RefSeq Version XM_015298084.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout guidance receptor 4 (ROBO4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298084.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCGGGCG GCTGGGCCAC GGCACTGGGG CTGGGGCTCT GGGTTACTGC ACTGCGCCTG 
    GGGGGCTGCC ACCCCTCCGG AGGGGACATG GAGTCACCCA CCACCCAGCG CCCAGCTGGG
    CAGGCGCTGG AGCTGGACTG CGTGCCCCCC GCCGGGCAGC CCGAGCCCCG CGTCACCTGG
    AGGAAGGACG GCGTCGCCCT GCGCCTGGCT GGGGGCCGTC ACCAGCTCAC ACGGGGGAAG
    CTGTGGGTGG CCTCGGCGCG CAGGAGCGAC GCGGGGGTCT ATGTCTGTGT GGCAGCCAAT
    GCAGCGGGCG AGCGGCACAG CCGGGGTGCC CACGTCACCA TCCTGGAGAA GCCGGTCATC
    GTGCGGCGGC CAAGCGATGT CACAGCAGTG GCCGGCAGCA CAGTGGAGCT GAGCTGCGGT
    GCACAGGGCG ACCCAGCACC ACGGGTTCAG TGGCATAAGG AGAGCGGGGA TCTGCCCTGG
    GGGAGGCACG AGGTGGACCA GGAGCACACA CTGCGACTCT ACGCGGTGAC GGCAGCCGAT
    GGTGGTGCCT ACGTCTGCAC AGCCCAGAGC CAGCTGGGCA GCACCGCTGC CACCGCCCGC
    CTCCGTGTGG AGGGGCAGCT GCCAGCAGGC TGGTGGGAGG CTACACGGCG GAACCTGCTG
    GCTGTGCAGC TGCAGCTGGA CAATGGCACT GCACTGCCCA CCAATGCCAC TGTCTGGCTC
    CGCTGGCGGA TGCTGACACC AGGGCCAGCT CCAGAGGGTT ACGTGGTGCT GTATCGCTGC
    CTGCTCCCTG CCAGCACTGC CTGGTCCCAG CAGGATGTGG GCAGGGAGCT CAGCACCATC
    ATCCCTGCAC TCAAGCGGGG CTACCAGTAT GAGTTCAAAG TCCGACCCTA TGCCGGTGGG
    ACCCAGGGCT TGGACAGCAA CAGCTGGCAC CTCTGGATCC CCGAAGAGGC AGTGCCAAGC
    GCGGCACCCC AGCGTGTCAC CGTGGGCCAA GCTGCACCGG GGAATGGTAC CGTCATCGTG
    AGCTGGGAGC CGCCTCCTCC TGATGCCCAC AATGGCATCA TTCGGGGCTA CCAGGTCTGG
    TCCCTGGGCG AGGGCTGGCT ACAACCCACC AACCATACAG TGGATGGAGG AACACGTCGC
    TTGGAGACCC TCCTGCATGG CCCTGGGGCC AGATACTGCA TCCAAGTAGC CGCGTTCAAT
    GGCGCAGGGC TGGGGATCCC CAGCAATGCC ACCTGTGGTG TCCTGGAGAT GATGGCAGGG
    AGCACCGGAG TGGTGCAGGT GCTGCAGCAG CCTGTGGTCA TCGCAGCAAC TGGCTCACTG
    CTGTGGCTGG CCCTGCTTGC CCTCCTCCTC CTCATCTGCC AGCGTCGTGC CAGCCAGGAC
    TCCATGGCAC AACATGGGCA GGAGGACGGT GATGTGCCAT GGCTCAGCAG CCCCTGGAAA
    CCTGTCTGTG TCCCTCACAA CCTCAGTGGC AGCAGCAGCC TCAGCAGCCG GCTCCTGGGA
    AGCGACAGCA GGGACCCCCA GCCCTCTCCC CTGACCTCGG AGCTGCCAAG CCTCAGCCCT
    GCGACGACCC CCAGCTGCAG CCACCTCTGC AGAGAACACC CCAAAACTAA CACCTCCAGC
    CTGGCACTGT GGGAGCACAT CCGCAAGCGA GAGCTGGACC GTACGCACAG CTCCCCAGTG
    CCGGTGGCTG GGCTCAGGGC CATGCCCGTG CCCCTGTGTG GCGGGGAGTG GGGGACACAT
    TTCAAATCAG TGGACAGATG GCCTCAGCAG AAAGGACAGG ATGGTGACAC CGCAATAGCA
    GGAGAGGACC CGTGGTGGCT GCCCACCATC AGCTCCCCAA AACCACGGCG CAGTTCACTG
    GCATCAATTG GTGCTGGGAT GCTGGCGGTA CCTGCAGGAC ATCCCCACGC CTGGCACCCC
    ACGGGGACCC GGTTCCCGGT CATGGGGTGC TCAAGGGACA CATCTCCAGT CACTGGGAAC
    CCCAGTGATA TATCCACGGT CACCAGGAAC CCTGGGGACA TGTCCCCAGT CACTAGGAAC
    ACCAAGAACA CACCTCTGAT CACCAGGAAC CACAGGGACA TGTCCTCAGT CACGAGGAGC
    CCCGTAGATA TGTCCCCTGC CACTGAGAAC CGCAGGGACA TCTTACTGGC CACAAGACCT
    AGCAGAGATG TGTCCCCGGT CACTGGGTGC CCCAAGGACA GATCCCCGGT CATTGGGCAC
    TCCAAGGAAA AGTCCCCAGC CCCCAGGAGC CCCAGGGACT TGTCCCCAGC CTCCAGTCAC
    TCCCTAGCCA CCACAGTTCA GGAGAACACG TCCCCGGTCA CTCAGCACCC CAGGGACATG
    TTCCCAGCCA CCAGGGGTCC CAGAGATGTG TTCCCAGCAG CCAGGCGCCC TGATGACATG
    TCCATGGTCC CCCAGAACCC CAGGGACACA TCTGTGCTCA CCAGACGACC CAGGGACATG
    CTGATGGCCA CCAAGGACAT GTCCCCAGGG CCCGGCCGCC TCTCACCAGC ACTCAGTGAT
    GGGGTCCTCA CCCCGCAGCG GGTGGCCGAG GACCTGGAGA GGGTGCAGGA GGCCCTCCGC
    CCCACGAACG CCCCCAGGCC AGAGGTGTCC CCAGCACGGC CCCAGCATGT CCCCGTGCCC
    CGGCCCCTGT CCCCTCCCCA CACCTACGGG TACATCTGCG GGCCTCCCGC CTCAGAGATG
    GGTGAGGGAG AGGACGAGGA GGAGGTGGAA GAAGAGGAGG AGGAAGAGAA GTCCGAGGTG
    TGGGGTCATG GGGGTTCGCC AGGGGGGTCT CTGCTGAACG GATGGGGGTC TGTCTCGGAG
    GACAATGTCC CCAGCACCCG CTGCAGCCTG GCCAGCTCCT CCGATGGCTC CTTCCTGCTT
    GATGCCAGCT TTGCCCGTGC GCTGGCTGTG GCCGTGGACA GCCTCTGCTT CAACCTCGAG
    GACAACGATG GGGGCGGTGA GGGAACTGCT GCCCCCCTGC ACCGCCTGGC ATCATCCCCT
    CTGTGCGGGG AGGTGGCCCT GGGGACGGGG ATGGGGATGG GGATGGGCGG CGTCTGGGCC
    AGGGTGGGCA GCCAGTGGGG TGGGCTCCTT TCCAAGGACA GGAGGGACAG GAGTGTGGCA
    GCCCCTGGGT TATACCAGTG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa45876
    Clone ID Related Accession (Same CDS sequence) XM_015298084.2 , XM_015298084.1
    Accession Version XM_015298084.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3144bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product roundabout homolog 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456007.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCGGGCG GCTGGGCCAC GGCACTGGGG CTGGGGCTCT GGGTTACTGC ACTGCGCCTG 
    GGGGGCTGCC ACCCCTCCGG AGGGGACATG GAGTCACCCA CCACCCAGCG CCCAGCTGGG
    CAGGCGCTGG AGCTGGACTG CGTGCCCCCC GCCGGGCAGC CCGAGCCCCG CGTCACCTGG
    AGGAAGGACG GCGTCGCCCT GCGCCTGGCT GGGGGCCGTC ACCAGCTCAC ACGGGGGAAG
    CTGTGGGTGG CCTCGGCGCG CAGGAGCGAC GCGGGGGTCT ATGTCTGTGT GGCAGCCAAT
    GCAGCGGGCG AGCGGCACAG CCGGGGTGCC CACGTCACCA TCCTGGAGAA GCCGGTCATC
    GTGCGGCGGC CAAGCGATGT CACAGCAGTG GCCGGCAGCA CAGTGGAGCT GAGCTGCGGT
    GCACAGGGCG ACCCAGCACC ACGGGTTCAG TGGCATAAGG AGAGCGGGGA TCTGCCCTGG
    GGGAGGCACG AGGTGGACCA GGAGCACACA CTGCGACTCT ACGCGGTGAC GGCAGCCGAT
    GGTGGTGCCT ACGTCTGCAC AGCCCAGAGC CAGCTGGGCA GCACCGCTGC CACCGCCCGC
    CTCCGTGTGG AGGGGCAGCT GCCAGCAGGC TGGTGGGAGG CTACACGGCG GAACCTGCTG
    GCTGTGCAGC TGCAGCTGGA CAATGGCACT GCACTGCCCA CCAATGCCAC TGTCTGGCTC
    CGCTGGCGGA TGCTGACACC AGGGCCAGCT CCAGAGGGTT ACGTGGTGCT GTATCGCTGC
    CTGCTCCCTG CCAGCACTGC CTGGTCCCAG CAGGATGTGG GCAGGGAGCT CAGCACCATC
    ATCCCTGCAC TCAAGCGGGG CTACCAGTAT GAGTTCAAAG TCCGACCCTA TGCCGGTGGG
    ACCCAGGGCT TGGACAGCAA CAGCTGGCAC CTCTGGATCC CCGAAGAGGC AGTGCCAAGC
    GCGGCACCCC AGCGTGTCAC CGTGGGCCAA GCTGCACCGG GGAATGGTAC CGTCATCGTG
    AGCTGGGAGC CGCCTCCTCC TGATGCCCAC AATGGCATCA TTCGGGGCTA CCAGGTCTGG
    TCCCTGGGCG AGGGCTGGCT ACAACCCACC AACCATACAG TGGATGGAGG AACACGTCGC
    TTGGAGACCC TCCTGCATGG CCCTGGGGCC AGATACTGCA TCCAAGTAGC CGCGTTCAAT
    GGCGCAGGGC TGGGGATCCC CAGCAATGCC ACCTGTGGTG TCCTGGAGAT GATGGCAGGG
    AGCACCGGAG TGGTGCAGGT GCTGCAGCAG CCTGTGGTCA TCGCAGCAAC TGGCTCACTG
    CTGTGGCTGG CCCTGCTTGC CCTCCTCCTC CTCATCTGCC AGCGTCGTGC CAGCCAGGAC
    TCCATGGCAC AACATGGGCA GGAGGACGGT GATGTGCCAT GGCTCAGCAG CCCCTGGAAA
    CCTGTCTGTG TCCCTCACAA CCTCAGTGGC AGCAGCAGCC TCAGCAGCCG GCTCCTGGGA
    AGCGACAGCA GGGACCCCCA GCCCTCTCCC CTGACCTCGG AGCTGCCAAG CCTCAGCCCT
    GCGACGACCC CCAGCTGCAG CCACCTCTGC AGAGAACACC CCAAAACTAA CACCTCCAGC
    CTGGCACTGT GGGAGCACAT CCGCAAGCGA GAGCTGGACC GTACGCACAG CTCCCCAGTG
    CCGGTGGCTG GGCTCAGGGC CATGCCCGTG CCCCTGTGTG GCGGGGAGTG GGGGACACAT
    TTCAAATCAG TGGACAGATG GCCTCAGCAG AAAGGACAGG ATGGTGACAC CGCAATAGCA
    GGAGAGGACC CGTGGTGGCT GCCCACCATC AGCTCCCCAA AACCACGGCG CAGTTCACTG
    GCATCAATTG GTGCTGGGAT GCTGGCGGTA CCTGCAGGAC ATCCCCACGC CTGGCACCCC
    ACGGGGACCC GGTTCCCGGT CATGGGGTGC TCAAGGGACA CATCTCCAGT CACTGGGAAC
    CCCAGTGATA TATCCACGGT CACCAGGAAC CCTGGGGACA TGTCCCCAGT CACTAGGAAC
    ACCAAGAACA CACCTCTGAT CACCAGGAAC CACAGGGACA TGTCCTCAGT CACGAGGAGC
    CCCGTAGATA TGTCCCCTGC CACTGAGAAC CGCAGGGACA TCTTACTGGC CACAAGACCT
    AGCAGAGATG TGTCCCCGGT CACTGGGTGC CCCAAGGACA GATCCCCGGT CATTGGGCAC
    TCCAAGGAAA AGTCCCCAGC CCCCAGGAGC CCCAGGGACT TGTCCCCAGC CTCCAGTCAC
    TCCCTAGCCA CCACAGTTCA GGAGAACACG TCCCCGGTCA CTCAGCACCC CAGGGACATG
    TTCCCAGCCA CCAGGGGTCC CAGAGATGTG TTCCCAGCAG CCAGGCGCCC TGATGACATG
    TCCATGGTCC CCCAGAACCC CAGGGACACA TCTGTGCTCA CCAGACGACC CAGGGACATG
    CTGATGGCCA CCAAGGACAT GTCCCCAGGG CCCGGCCGCC TCTCACCAGC ACTCAGTGAT
    GGGGTCCTCA CCCCGCAGCG GGTGGCCGAG GACCTGGAGA GGGTGCAGGA GGCCCTCCGC
    CCCACGAACG CCCCCAGGCC AGAGGTGTCC CCAGCACGGC CCCAGCATGT CCCCGTGCCC
    CGGCCCCTGT CCCCTCCCCA CACCTACGGG TACATCTGCG GGCCTCCCGC CTCAGAGATG
    GGTGAGGGAG AGGACGAGGA GGAGGTGGAA GAAGAGGAGG AGGAAGAGAA GTCCGAGGTG
    TGGGGTCATG GGGGTTCGCC AGGGGGGTCT CTGCTGAACG GATGGGGGTC TGTCTCGGAG
    GACAATGTCC CCAGCACCCG CTGCAGCCTG GCCAGCTCCT CCGATGGCTC CTTCCTGCTT
    GATGCCAGCT TTGCCCGTGC GCTGGCTGTG GCCGTGGACA GCCTCTGCTT CAACCTCGAG
    GACAACGATG GGGGCGGTGA GGGAACTGCT GCCCCCCTGC ACCGCCTGGC ATCATCCCCT
    CTGTGCGGGG AGGTGGCCCT GGGGACGGGG ATGGGGATGG GGATGGGCGG CGTCTGGGCC
    AGGGTGGGCA GCCAGTGGGG TGGGCTCCTT TCCAAGGACA GGAGGGACAG GAGTGTGGCA
    GCCCCTGGGT TATACCAGTG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015153570.1
    CDS335..3478
    Translation

    Target ORF information:

    RefSeq Version XM_015298084.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout guidance receptor 4 (ROBO4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298084.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCGGGCG GCTGGGCCAC GGCACTGGGG CTGGGGCTCT GGGTTACTGC ACTGCGCCTG 
    GGGGGCTGCC ACCCCTCCGG AGGGGACATG GAGTCACCCA CCACCCAGCG CCCAGCTGGG
    CAGGCGCTGG AGCTGGACTG CGTGCCCCCC GCCGGGCAGC CCGAGCCCCG CGTCACCTGG
    AGGAAGGACG GCGTCGCCCT GCGCCTGGCT GGGGGCCGTC ACCAGCTCAC ACGGGGGAAG
    CTGTGGGTGG CCTCGGCGCG CAGGAGCGAC GCGGGGGTCT ATGTCTGTGT GGCAGCCAAT
    GCAGCGGGCG AGCGGCACAG CCGGGGTGCC CACGTCACCA TCCTGGAGAA GCCGGTCATC
    GTGCGGCGGC CAAGCGATGT CACAGCAGTG GCCGGCAGCA CAGTGGAGCT GAGCTGCGGT
    GCACAGGGCG ACCCAGCACC ACGGGTTCAG TGGCATAAGG AGAGCGGGGA TCTGCCCTGG
    GGGAGGCACG AGGTGGACCA GGAGCACACA CTGCGACTCT ACGCGGTGAC GGCAGCCGAT
    GGTGGTGCCT ACGTCTGCAC AGCCCAGAGC CAGCTGGGCA GCACCGCTGC CACCGCCCGC
    CTCCGTGTGG AGGGGCAGCT GCCAGCAGGC TGGTGGGAGG CTACACGGCG GAACCTGCTG
    GCTGTGCAGC TGCAGCTGGA CAATGGCACT GCACTGCCCA CCAATGCCAC TGTCTGGCTC
    CGCTGGCGGA TGCTGACACC AGGGCCAGCT CCAGAGGGTT ACGTGGTGCT GTATCGCTGC
    CTGCTCCCTG CCAGCACTGC CTGGTCCCAG CAGGATGTGG GCAGGGAGCT CAGCACCATC
    ATCCCTGCAC TCAAGCGGGG CTACCAGTAT GAGTTCAAAG TCCGACCCTA TGCCGGTGGG
    ACCCAGGGCT TGGACAGCAA CAGCTGGCAC CTCTGGATCC CCGAAGAGGC AGTGCCAAGC
    GCGGCACCCC AGCGTGTCAC CGTGGGCCAA GCTGCACCGG GGAATGGTAC CGTCATCGTG
    AGCTGGGAGC CGCCTCCTCC TGATGCCCAC AATGGCATCA TTCGGGGCTA CCAGGTCTGG
    TCCCTGGGCG AGGGCTGGCT ACAACCCACC AACCATACAG TGGATGGAGG AACACGTCGC
    TTGGAGACCC TCCTGCATGG CCCTGGGGCC AGATACTGCA TCCAAGTAGC CGCGTTCAAT
    GGCGCAGGGC TGGGGATCCC CAGCAATGCC ACCTGTGGTG TCCTGGAGAT GATGGCAGGG
    AGCACCGGAG TGGTGCAGGT GCTGCAGCAG CCTGTGGTCA TCGCAGCAAC TGGCTCACTG
    CTGTGGCTGG CCCTGCTTGC CCTCCTCCTC CTCATCTGCC AGCGTCGTGC CAGCCAGGAC
    TCCATGGCAC AACATGGGCA GGAGGACGGT GATGTGCCAT GGCTCAGCAG CCCCTGGAAA
    CCTGTCTGTG TCCCTCACAA CCTCAGTGGC AGCAGCAGCC TCAGCAGCCG GCTCCTGGGA
    AGCGACAGCA GGGACCCCCA GCCCTCTCCC CTGACCTCGG AGCTGCCAAG CCTCAGCCCT
    GCGACGACCC CCAGCTGCAG CCACCTCTGC AGAGAACACC CCAAAACTAA CACCTCCAGC
    CTGGCACTGT GGGAGCACAT CCGCAAGCGA GAGCTGGACC GTACGCACAG CTCCCCAGTG
    CCGGTGGCTG GGCTCAGGGC CATGCCCGTG CCCCTGTGTG GCGGGGAGTG GGGGACACAT
    TTCAAATCAG TGGACAGATG GCCTCAGCAG AAAGGACAGG ATGGTGACAC CGCAATAGCA
    GGAGAGGACC CGTGGTGGCT GCCCACCATC AGCTCCCCAA AACCACGGCG CAGTTCACTG
    GCATCAATTG GTGCTGGGAT GCTGGCGGTA CCTGCAGGAC ATCCCCACGC CTGGCACCCC
    ACGGGGACCC GGTTCCCGGT CATGGGGTGC TCAAGGGACA CATCTCCAGT CACTGGGAAC
    CCCAGTGATA TATCCACGGT CACCAGGAAC CCTGGGGACA TGTCCCCAGT CACTAGGAAC
    ACCAAGAACA CACCTCTGAT CACCAGGAAC CACAGGGACA TGTCCTCAGT CACGAGGAGC
    CCCGTAGATA TGTCCCCTGC CACTGAGAAC CGCAGGGACA TCTTACTGGC CACAAGACCT
    AGCAGAGATG TGTCCCCGGT CACTGGGTGC CCCAAGGACA GATCCCCGGT CATTGGGCAC
    TCCAAGGAAA AGTCCCCAGC CCCCAGGAGC CCCAGGGACT TGTCCCCAGC CTCCAGTCAC
    TCCCTAGCCA CCACAGTTCA GGAGAACACG TCCCCGGTCA CTCAGCACCC CAGGGACATG
    TTCCCAGCCA CCAGGGGTCC CAGAGATGTG TTCCCAGCAG CCAGGCGCCC TGATGACATG
    TCCATGGTCC CCCAGAACCC CAGGGACACA TCTGTGCTCA CCAGACGACC CAGGGACATG
    CTGATGGCCA CCAAGGACAT GTCCCCAGGG CCCGGCCGCC TCTCACCAGC ACTCAGTGAT
    GGGGTCCTCA CCCCGCAGCG GGTGGCCGAG GACCTGGAGA GGGTGCAGGA GGCCCTCCGC
    CCCACGAACG CCCCCAGGCC AGAGGTGTCC CCAGCACGGC CCCAGCATGT CCCCGTGCCC
    CGGCCCCTGT CCCCTCCCCA CACCTACGGG TACATCTGCG GGCCTCCCGC CTCAGAGATG
    GGTGAGGGAG AGGACGAGGA GGAGGTGGAA GAAGAGGAGG AGGAAGAGAA GTCCGAGGTG
    TGGGGTCATG GGGGTTCGCC AGGGGGGTCT CTGCTGAACG GATGGGGGTC TGTCTCGGAG
    GACAATGTCC CCAGCACCCG CTGCAGCCTG GCCAGCTCCT CCGATGGCTC CTTCCTGCTT
    GATGCCAGCT TTGCCCGTGC GCTGGCTGTG GCCGTGGACA GCCTCTGCTT CAACCTCGAG
    GACAACGATG GGGGCGGTGA GGGAACTGCT GCCCCCCTGC ACCGCCTGGC ATCATCCCCT
    CTGTGCGGGG AGGTGGCCCT GGGGACGGGG ATGGGGATGG GGATGGGCGG CGTCTGGGCC
    AGGGTGGGCA GCCAGTGGGG TGGGCTCCTT TCCAAGGACA GGAGGGACAG GAGTGTGGCA
    GCCCCTGGGT TATACCAGTG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.