GUCY2F cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	GUCY2F
Entrez Gene ID	102402009
Full Name	guanylate cyclase 2F, retinal
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006071580.2	XP_006071642.2	retinal guanylyl cyclase 2
XM_006071580.1	XP_006071642.1	retinal guanylyl cyclase 2

Pathways from BioSystems
Gene Ontology

The following GUCY2F gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GUCY2F cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBu25985	XM_006071580.1 Latest version!	Bubalus bubalis guanylate cyclase 2F, retinal (GUCY2F), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBu73679	XM_006071580.2 Latest version!	Bubalus bubalis guanylate cyclase 2F, retinal (GUCY2F), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GUCY2F ( XM_006071580.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006071642.1 Bubalus bubalis guanylate cyclase 2F, retinal (GUCY2F), mRNA.

Price & Availability↑

CloneID	OBu25985
Clone ID Related Accession (Same CDS sequence)	XM_006071580.1
Accession Version	XM_006071580.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	retinal guanylyl cyclase 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785447.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTTGG GACCCTGGCC TTTCTCCCAC CTTATGCTCT GGTTCGCGAC TCTGGGAAGA 
CAGCGAGGAC AACATGGCCT TGCATCTTTC AAGCTCCTGT GGTGTTTGTG GCTCTTGGTC 
CTTATGTCTC TCCCACTGCA GGTGTGGGGG CCACCCTACA AGATAGGGGT GGTTGGCCCT 
TGGACTTGTG ATCCATTGTT CTCAAAGGCC CTGCCTGAGA TTGCTGCTCA GTTAGCCACT 
GAGCGGATCA ACAAGGACCC AGCCTTGGAC CTGGGTCACT CTCTTGAATA TGTGATTTTC 
AATGAAGACT GCCAGGCTTC CAGGGCTCTC TCCAGTTTCA TTTCCCACCA CCAGATGGCC 
TCAGGGTTTA TTGGACCTGC CAACCCTGGC TACTGCGAGG CAGCCTCGCT CCTGGGAAAC 
AGCTGGGACA AAGGGATTTT CTCCTGGGCT TGCGTGAATT ATGAATTAGA CAGTAAAAAT 
AGCCACCCGA CTTTTTCTCG GACTCTCCCT TCTCCCATCC GGGTGCTGCT AACTGTCATG 
AAATATTTCC AGTGGGCTCA CGCTGGAGTC ATTTCCTCAG ATGAAGACAT TTGGGTGCAC 
ACAGCCTATC GAGTTGCAAG TGCTCTTCGG AGCCGCGGCC TACCAGTAGG GGTCGTCCTG 
ACCACAGGAC AAGACAGCCA AAGCATCCAG AAAGCTCTCC AGCAGATTCG CCAGGCAGAT 
AGAATTCGCA TAATCATCAT GTGTATGCAT TCGACTTTGA TTGGGGGAGA GACTCAGACG 
CATCTCTTGG AATGGGCTCA CGATCTGCAA ATGACTGATG GCACCTATGT CTTTGTTCCC 
TATGACACCC TGCTCTACAG TTTACCTTAT AAGCATATCC CCTACCAGGT TTTAAGGAAC 
AACCCAAAAC TCCGGGAAGC CTATGATGCT GTGTTGACCA TCACAGTAGA GTCCCAAGAA 
AAGACCTTCT ATCAAGCCTT TGAGGAGGCA GCAGCTAGAG GTGAAATTCC TGAGAAGCTG 
GAGTCAGATC AAGTTTCGCC GTTGTTTGGA ACCATCTACA ATTCAATTTA CTTTATCGCA 
CAAGCCATGA ATAATGCTAT GAAAGAAAAT GGATGGGCTA GTGCTGCCAG CCTGGTTCAG 
CATTCCAGAA ACATGCAGTT CTATGGATTC AACCAGTTGA TAAGGACAGA TTCAAATGGA 
AATGGAATTT CAGAATATGT AATCCTGGAC ACCAACTGGA AAGAATGGGA ACTCCATAGT 
ACCTACACTG TGGACATGGA AACGGAGCTG CTACGATTCG GAGAGACCCC TATTCACTTT 
CCTGGTGGCA GGCCCCCTCG AGCAGATGCA CAATGCTGGT TTGCAGATGG GAAGATCTGC 
CAAGGAGGCA TCAACCCTAC CTTTGCTTTG ATGGTCTGCC TTGCTTTGCT TATAGCCCTG 
CTGTCTATTA ATGGATTTGC TTACTTTATA AGACATCGTA TAAATAAAAT CCAGTTGATT 
AAAGGACCCA ACAGAATTCT ACTGACTTTG GAGGATGTGA CATTTATCAA TCCCCACTTT 
GGCAGTAAGA GAGGAAGTCA CGCCAGTGTA AGTTTCCAGA TCACGTCGGA GGTCCAGAGT 
GGAAGGTCCC CAAGACTCTC CTTTTCTTCA GGGAGTCTAA CTCCAGCTAC CTGTGAAAAC 
TCCAACATAG CGATTTATGA GGGTGATTGG GTATGGCTGA AAAAATTCCC CTCAGGAAAT 
TTTGGAGACA TTAAATCCGT CGAATCAAGT GCAAGTGACA TATTTGAAAT GATGAAGGAC 
CTTGCGATAC ATGAGAATAT TAACCCTTTG GTGGGCTTCT TCTATGATTC GGGGGTATTT 
GCCATTGTGA CAGAATTCTG TTCCCGAAGG AGCCTGGAAG ACATACTGAT GAATCAGGAT 
GTGAAACTAG ATTGGATGTT TAAATCATCA CTCCTGCTGG ATCTCATCAA GGGCATGAAG 
TACTTACACC ACAGAGAGTT TGCTCATGGG AGGCTGAAGT CTCGGAACTG TGTGGTGGAT 
GGGCGTTTTG TACTCAAAGT GACAGATTAT GGCTTTAATG ACATCTTGGA AACGCTAAGA 
CTCTCCCAAG AGGAACCTTC TGCAGAAGAG TTGCTGTGGA CAGCCCCTGA ACTGTTGAGA 
GCCCCGAGGG GCAGCAGGTT ATGTTCTTTT GCAGGAGACG TCTATAGCTT TGCCATCATC 
ATGCAGGAAG TGATGGTCCG GGGCACCCCA TTCTGCATGA TGGATCTGCC TGCCAAAGAA 
ATCATAGAAA GAATTAAGAA GTCTCCTCCA GTGTACAGAC CAGTGGTTCC TCCTGAGCAT 
GCACCCCCAG AATGTCTCCA GCTGATGAAG CAGTGCTGGG CTGAGGCTGC AGAACAACGA 
CCAACATTTG ATGAAATATT TAACCAGTTT AAAACTTTCA ATAAAGGGAA GAAGACTAAT 
ATTATTGACT CTATGCTTCG GATGCTGGAG CAATATTCTA GCAACTTGGA AGATTTGATC 
CAGGAGCGAA CTGAAGAGCT GGAAATTGAA AAACAGAAAA CGGAAAAGCT CCTAACACAG 
ATGCTACCAC CATCAGTTGC TGAATCTCTC AAAAAGGGCT GCACAGTCGA ACCGGAGGGC 
TTTGATTTGG TCACCTTGTA CTTCAGCGAC ATTGTGGGCT TCACTACCAT CTCAGCCATG 
AGTGAGCCCA TTGAGGTGGT GGATCTTCTC AATGACCTGT ACACCCTCTT TGATGCCATC 
ATTGGCAGTC ATGATGTCTA CAAGGTAGAG ACTATTGGAG ATGCTTACAT GGTGGCCTCA 
GGCCTCCCAA AGAGGAATGG CATGAGGCAT GCAGCCGAGA TTGCAAACAT GTCCCTAGAC 
ATCCTCAGCT CTGTGGGTAC CTTCAAGATG CGGCACATGC CAGAGGTGCC GGTCCGGATT 
CGAATAGGCC TGCACTCAGG GCCGGTTGTT GCTGGAGTGG TGGGCCTCAC TATGCCCAGA 
TACTGCTTGT TTGGAGATAC TGTGAACACA GCTTCTCGGA TGGAATCAAC AGGGTTACCT 
TATCGCATTC ATGTCAGTCA TAGCACAGTG ACAATTCTTC GAACCCTGGG TGAAGGCTAT 
GAAGTGGAGC TTCGAGGAAG AACAGAGCTC AAGGGCAAAG GCACAGAAGA GACCTTCTGG 
CTAGTTGGGA AAAAGGGCTT CACGAAGCCC CTTCCTGTGC CCCCACCAGT GGGCAAAGAT 
GGGCAAGTGG GCCATGGCCT TCAACCAGTA GAGATTGCAG CCTTCCAAAG AAGAAAAGCA 
GAAAAGCAGC TGGTGAGAAA CAAGCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006071642.1
CDS	1..3330
Translation	MFLGPWPFSHLMLWFATLGRQRGQHGLASFKLLWCLWLLVLMSLPLQVWGPPYKIGVVGPWTCDPLFSKALPEIAAQLATERINKDPALDLGHSLEYVIFNEDCQASRALSSFISHHQMASGFIGPANPGYCEAASLLGNSWDKGIFSWACVNYELDSKNSHPTFSRTLPSPIRVLLTVMKYFQWAHAGVISSDEDIWVHTAYRVASALRSRGLPVGVVLTTGQDSQSIQKALQQIRQADRIRIIIMCMHSTLIGGETQTHLLEWAHDLQMTDGTYVFVPYDTLLYSLPYKHIPYQVLRNNPKLREAYDAVLTITVESQEKTFYQAFEEAAARGEIPEKLESDQVSPLFGTIYNSIYFIAQAMNNAMKENGWASAASLVQHSRNMQFYGFNQLIRTDSNGNGISEYVILDTNWKEWELHSTYTVDMETELLRFGETPIHFPGGRPPRADAQCWFADGKICQGGINPTFALMVCLALLIALLSINGFAYFIRHRINKIQLIKGPNRILLTLEDVTFINPHFGSKRGSHASVSFQITSEVQSGRSPRLSFSSGSLTPATCENSNIAIYEGDWVWLKKFPSGNFGDIKSVESSASDIFEMMKDLAIHENINPLVGFFYDSGVFAIVTEFCSRRSLEDILMNQDVKLDWMFKSSLLLDLIKGMKYLHHREFAHGRLKSRNCVVDGRFVLKVTDYGFNDILETLRLSQEEPSAEELLWTAPELLRAPRGSRLCSFAGDVYSFAIIMQEVMVRGTPFCMMDLPAKEIIERIKKSPPVYRPVVPPEHAPPECLQLMKQCWAEAAEQRPTFDEIFNQFKTFNKGKKTNIIDSMLRMLEQYSSNLEDLIQERTEELEIEKQKTEKLLTQMLPPSVAESLKKGCTVEPEGFDLVTLYFSDIVGFTTISAMSEPIEVVDLLNDLYTLFDAIIGSHDVYKVETIGDAYMVASGLPKRNGMRHAAEIANMSLDILSSVGTFKMRHMPEVPVRIRIGLHSGPVVAGVVGLTMPRYCLFGDTVNTASRMESTGLPYRIHVSHSTVTILRTLGEGYEVELRGRTELKGKGTEETFWLVGKKGFTKPLPVPPPVGKDGQVGHGLQPVEIAAFQRRKAEKQLVRNKP

Target ORF information:

RefSeq Version	XM_006071580.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis guanylate cyclase 2F, retinal (GUCY2F), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006071580.1

ORF Insert Sequence:

ATGTTCTTGG GACCCTGGCC TTTCTCCCAC CTTATGCTCT GGTTCGCGAC TCTGGGAAGA 
CAGCGAGGAC AACATGGCCT TGCATCTTTC AAGCTCCTGT GGTGTTTGTG GCTCTTGGTC 
CTTATGTCTC TCCCACTGCA GGTGTGGGGG CCACCCTACA AGATAGGGGT GGTTGGCCCT 
TGGACTTGTG ATCCATTGTT CTCAAAGGCC CTGCCTGAGA TTGCTGCTCA GTTAGCCACT 
GAGCGGATCA ACAAGGACCC AGCCTTGGAC CTGGGTCACT CTCTTGAATA TGTGATTTTC 
AATGAAGACT GCCAGGCTTC CAGGGCTCTC TCCAGTTTCA TTTCCCACCA CCAGATGGCC 
TCAGGGTTTA TTGGACCTGC CAACCCTGGC TACTGCGAGG CAGCCTCGCT CCTGGGAAAC 
AGCTGGGACA AAGGGATTTT CTCCTGGGCT TGCGTGAATT ATGAATTAGA CAGTAAAAAT 
AGCCACCCGA CTTTTTCTCG GACTCTCCCT TCTCCCATCC GGGTGCTGCT AACTGTCATG 
AAATATTTCC AGTGGGCTCA CGCTGGAGTC ATTTCCTCAG ATGAAGACAT TTGGGTGCAC 
ACAGCCTATC GAGTTGCAAG TGCTCTTCGG AGCCGCGGCC TACCAGTAGG GGTCGTCCTG 
ACCACAGGAC AAGACAGCCA AAGCATCCAG AAAGCTCTCC AGCAGATTCG CCAGGCAGAT 
AGAATTCGCA TAATCATCAT GTGTATGCAT TCGACTTTGA TTGGGGGAGA GACTCAGACG 
CATCTCTTGG AATGGGCTCA CGATCTGCAA ATGACTGATG GCACCTATGT CTTTGTTCCC 
TATGACACCC TGCTCTACAG TTTACCTTAT AAGCATATCC CCTACCAGGT TTTAAGGAAC 
AACCCAAAAC TCCGGGAAGC CTATGATGCT GTGTTGACCA TCACAGTAGA GTCCCAAGAA 
AAGACCTTCT ATCAAGCCTT TGAGGAGGCA GCAGCTAGAG GTGAAATTCC TGAGAAGCTG 
GAGTCAGATC AAGTTTCGCC GTTGTTTGGA ACCATCTACA ATTCAATTTA CTTTATCGCA 
CAAGCCATGA ATAATGCTAT GAAAGAAAAT GGATGGGCTA GTGCTGCCAG CCTGGTTCAG 
CATTCCAGAA ACATGCAGTT CTATGGATTC AACCAGTTGA TAAGGACAGA TTCAAATGGA 
AATGGAATTT CAGAATATGT AATCCTGGAC ACCAACTGGA AAGAATGGGA ACTCCATAGT 
ACCTACACTG TGGACATGGA AACGGAGCTG CTACGATTCG GAGAGACCCC TATTCACTTT 
CCTGGTGGCA GGCCCCCTCG AGCAGATGCA CAATGCTGGT TTGCAGATGG GAAGATCTGC 
CAAGGAGGCA TCAACCCTAC CTTTGCTTTG ATGGTCTGCC TTGCTTTGCT TATAGCCCTG 
CTGTCTATTA ATGGATTTGC TTACTTTATA AGACATCGTA TAAATAAAAT CCAGTTGATT 
AAAGGACCCA ACAGAATTCT ACTGACTTTG GAGGATGTGA CATTTATCAA TCCCCACTTT 
GGCAGTAAGA GAGGAAGTCA CGCCAGTGTA AGTTTCCAGA TCACGTCGGA GGTCCAGAGT 
GGAAGGTCCC CAAGACTCTC CTTTTCTTCA GGGAGTCTAA CTCCAGCTAC CTGTGAAAAC 
TCCAACATAG CGATTTATGA GGGTGATTGG GTATGGCTGA AAAAATTCCC CTCAGGAAAT 
TTTGGAGACA TTAAATCCGT CGAATCAAGT GCAAGTGACA TATTTGAAAT GATGAAGGAC 
CTTGCGATAC ATGAGAATAT TAACCCTTTG GTGGGCTTCT TCTATGATTC GGGGGTATTT 
GCCATTGTGA CAGAATTCTG TTCCCGAAGG AGCCTGGAAG ACATACTGAT GAATCAGGAT 
GTGAAACTAG ATTGGATGTT TAAATCATCA CTCCTGCTGG ATCTCATCAA GGGCATGAAG 
TACTTACACC ACAGAGAGTT TGCTCATGGG AGGCTGAAGT CTCGGAACTG TGTGGTGGAT 
GGGCGTTTTG TACTCAAAGT GACAGATTAT GGCTTTAATG ACATCTTGGA AACGCTAAGA 
CTCTCCCAAG AGGAACCTTC TGCAGAAGAG TTGCTGTGGA CAGCCCCTGA ACTGTTGAGA 
GCCCCGAGGG GCAGCAGGTT ATGTTCTTTT GCAGGAGACG TCTATAGCTT TGCCATCATC 
ATGCAGGAAG TGATGGTCCG GGGCACCCCA TTCTGCATGA TGGATCTGCC TGCCAAAGAA 
ATCATAGAAA GAATTAAGAA GTCTCCTCCA GTGTACAGAC CAGTGGTTCC TCCTGAGCAT 
GCACCCCCAG AATGTCTCCA GCTGATGAAG CAGTGCTGGG CTGAGGCTGC AGAACAACGA 
CCAACATTTG ATGAAATATT TAACCAGTTT AAAACTTTCA ATAAAGGGAA GAAGACTAAT 
ATTATTGACT CTATGCTTCG GATGCTGGAG CAATATTCTA GCAACTTGGA AGATTTGATC 
CAGGAGCGAA CTGAAGAGCT GGAAATTGAA AAACAGAAAA CGGAAAAGCT CCTAACACAG 
ATGCTACCAC CATCAGTTGC TGAATCTCTC AAAAAGGGCT GCACAGTCGA ACCGGAGGGC 
TTTGATTTGG TCACCTTGTA CTTCAGCGAC ATTGTGGGCT TCACTACCAT CTCAGCCATG 
AGTGAGCCCA TTGAGGTGGT GGATCTTCTC AATGACCTGT ACACCCTCTT TGATGCCATC 
ATTGGCAGTC ATGATGTCTA CAAGGTAGAG ACTATTGGAG ATGCTTACAT GGTGGCCTCA 
GGCCTCCCAA AGAGGAATGG CATGAGGCAT GCAGCCGAGA TTGCAAACAT GTCCCTAGAC 
ATCCTCAGCT CTGTGGGTAC CTTCAAGATG CGGCACATGC CAGAGGTGCC GGTCCGGATT 
CGAATAGGCC TGCACTCAGG GCCGGTTGTT GCTGGAGTGG TGGGCCTCAC TATGCCCAGA 
TACTGCTTGT TTGGAGATAC TGTGAACACA GCTTCTCGGA TGGAATCAAC AGGGTTACCT 
TATCGCATTC ATGTCAGTCA TAGCACAGTG ACAATTCTTC GAACCCTGGG TGAAGGCTAT 
GAAGTGGAGC TTCGAGGAAG AACAGAGCTC AAGGGCAAAG GCACAGAAGA GACCTTCTGG 
CTAGTTGGGA AAAAGGGCTT CACGAAGCCC CTTCCTGTGC CCCCACCAGT GGGCAAAGAT 
GGGCAAGTGG GCCATGGCCT TCAACCAGTA GAGATTGCAG CCTTCCAAAG AAGAAAAGCA 
GAAAAGCAGC TGGTGAGAAA CAAGCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY2F ( XM_006071580.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006071642.2 Bubalus bubalis guanylate cyclase 2F, retinal (GUCY2F), mRNA.

Price & Availability↑

CloneID	OBu73679
Clone ID Related Accession (Same CDS sequence)	XM_006071580.2
Accession Version	XM_006071580.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	retinal guanylyl cyclase 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006071580.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTTGG GACCCTGGCC TTTCTCCCAC CTTATGCTCT GGTTCGCGAC TCTGGGAAGA 
CAGCGAGGAC AACATGGCCT TGCATCTTTC AAGCTCCTGT GGTGTTTGTG GCTCTTGGTC 
CTTATGTCTC TCCCACTGCA GGTGTGGGGG CCACCCTACA AGATAGGGGT GGTTGGCCCT 
TGGACTTGTG ATCCATTGTT CTCAAAGGCC CTGCCTGAGA TTGCTGCTCA GTTAGCCACT 
GAGCGGATCA ACAAGGACCC AGCCTTGGAC CTGGGTCACT CTCTTGAATA TGTGATTTTC 
AATGAAGACT GCCAGGCTTC CAGGGCTCTC TCCAGTTTCA TTTCCCACCA CCAGATGGCC 
TCAGGGTTTA TTGGACCTGC CAACCCTGGC TACTGCGAGG CAGCCTCGCT CCTGGGAAAC 
AGCTGGGACA AAGGGATTTT CTCCTGGGCT TGCGTGAATT ATGAATTAGA CAGTAAAAAT 
AGCCACCCGA CTTTTTCTCG GACTCTCCCT TCTCCCATCC GGGTGCTGCT AACTGTCATG 
AAATATTTCC AGTGGGCTCA CGCTGGAGTC ATTTCCTCAG ATGAAGACAT TTGGGTGCAC 
ACAGCCTATC GAGTTGCAAG TGCTCTTCGG AGCCGCGGCC TACCAGTAGG GGTCGTCCTG 
ACCACAGGAC AAGACAGCCA AAGCATCCAG AAAGCTCTCC AGCAGATTCG CCAGGCAGAT 
AGAATTCGCA TAATCATCAT GTGTATGCAT TCGACTTTGA TTGGGGGAGA GACTCAGACG 
CATCTCTTGG AATGGGCTCA CGATCTGCAA ATGACTGATG GCACCTATGT CTTTGTTCCC 
TATGACACCC TGCTCTACAG TTTACCTTAT AAGCATATCC CCTACCAGGT TTTAAGGAAC 
AACCCAAAAC TCCGGGAAGC CTATGATGCT GTGTTGACCA TCACAGTAGA GTCCCAAGAA 
AAGACCTTCT ATCAAGCCTT TGAGGAGGCA GCAGCTAGAG GTGAAATTCC TGAGAAGCTG 
GAGTCAGATC AAGTTTCGCC GTTGTTTGGA ACCATCTACA ATTCAATTTA CTTTATCGCA 
CAAGCCATGA ATAATGCTAT GAAAGAAAAT GGATGGGCTA GTGCTGCCAG CCTGGTTCAG 
CATTCCAGAA ACATGCAGTT CTATGGATTC AACCAGTTGA TAAGGACAGA TTCAAATGGA 
AATGGAATTT CAGAATATGT AATCCTGGAC ACCAACTGGA AAGAATGGGA ACTCCATAGT 
ACCTACACTG TGGACATGGA AACGGAGCTG CTACGATTCG GAGAGACCCC TATTCACTTT 
CCTGGTGGCA GGCCCCCTCG AGCAGATGCA CAATGCTGGT TTGCAGATGG GAAGATCTGC 
CAAGGAGGCA TCAACCCTAC CTTTGCTTTG ATGGTCTGCC TTGCTTTGCT TATAGCCCTG 
CTGTCTATTA ATGGATTTGC TTACTTTATA AGACATCGTA TAAATAAAAT CCAGTTGATT 
AAAGGACCCA ACAGAATTCT ACTGACTTTG GAGGATGTGA CATTTATCAA TCCCCACTTT 
GGCAGTAAGA GAGGAAGTCA CGCCAGTGTA AGTTTCCAGA TCACGTCGGA GGTCCAGAGT 
GGAAGGTCCC CAAGACTCTC CTTTTCTTCA GGGAGTCTAA CTCCAGCTAC CTGTGAAAAC 
TCCAACATAG CGATTTATGA GGGTGATTGG GTATGGCTGA AAAAATTCCC CTCAGGAAAT 
TTTGGAGACA TTAAATCCGT CGAATCAAGT GCAAGTGACA TATTTGAAAT GATGAAGGAC 
CTGCGTCATG AGAATATTAA CCCTTTGGTG GGCTTCTTCT ATGATTCGGG GGTATTTGCC 
ATTGTGACAG AATTCTGTTC CCGAAGGAGC CTGGAAGACA TACTGATGAA TCAGGATGTG 
AAACTAGATT GGATGTTTAA ATCATCACTC CTGCTGGATC TCATCAAGGG CATGAAGTAC 
TTACACCACA GAGAGTTTGC TCATGGGAGG CTGAAGTCTC GGAACTGTGT GGTGGATGGG 
CGTTTTGTAC TCAAAGTGAC AGATTATGGC TTTAATGACA TCTTGGAAAC GCTAAGACTC 
TCCCAAGAGG AACCTTCTGC AGAAGAGTTG CTGTGGACAG CCCCTGAACT GTTGAGAGCC 
CCGAGGGGCA GCAGGTTATG TTCTTTTGCA GGAGACGTCT ATAGCTTTGC CATCATCATG 
CAGGAAGTGA TGGTCCGGGG CACCCCATTC TGCATGATGG ATCTGCCTGC CAAAGAAATC 
ATAGAAAGAA TTAAGAAGTC TCCTCCAGTG TACAGACCAG TGGTTCCTCC TGAGCATGCA 
CCCCCAGAAT GTCTCCAGCT GATGAAGCAG TGCTGGGCTG AGGCTGCAGA ACAACGACCA 
ACATTTGATG AAATATTTAA CCAGTTTAAA ACTTTCAATA AAGGGAAGAA GACTAATATT 
ATTGACTCTA TGCTTCGGAT GCTGGAGCAA TATTCTAGCA ACTTGGAAGA TTTGATCCAG 
GAGCGAACTG AAGAGCTGGA AATTGAAAAA CAGAAAACGG AAAAGCTCCT AACACAGATG 
CTACCACCAT CAGTTGCTGA ATCTCTCAAA AAGGGCTGCA CAGTCGAACC GGAGGGCTTT 
GATTTGGTCA CCTTGTACTT CAGCGACATT GTGGGCTTCA CTACCATCTC AGCCATGAGT 
GAGCCCATTG AGGTGGTGGA TCTTCTCAAT GACCTGTACA CCCTCTTTGA TGCCATCATT 
GGCAGTCATG ATGTCTACAA GGTAGAGACT ATTGGAGATG CTTACATGGT GGCCTCAGGC 
CTCCCAAAGA GGAATGGCAT GAGGCATGCA GCCGAGATTG CAAACATGTC CCTAGACATC 
CTCAGCTCTG TGGGTACCTT CAAGATGCGG CACATGCCAG AGGTGCCGGT CCGGATTCGA 
ATAGGCCTGC ACTCAGGGCC GGTTGTTGCT GGAGTGGTGG GCCTCACTAT GCCCAGATAC 
TGCTTGTTTG GAGATACTGT GAACACAGCT TCTCGGATGG AATCAACAGG GTTACCTTAT 
CGCATTCATG TCAGTCATAG CACAGTGACA ATTCTTCGAA CCCTGGGTGA AGGCTATGAA 
GTGGAGCTTC GAGGAAGAAC AGAGCTCAAG GGCAAAGGCA CAGAAGAGAC CTTCTGGCTA 
GTTGGGAAAA AGGGCTTCAC GAAGCCCCTT CCTGTGCCCC CACCAGTGGG CAAAGATGGG 
CAAGTGGGCC ATGGCCTTCA ACCAGTAGAG ATTGCAGCCT TCCAAAGAAG AAAAGCAGAA 
AAGCAGCTGG TGAGAAACAA GCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006071642.2
CDS	80..3406
Translation	MFLGPWPFSHLMLWFATLGRQRGQHGLASFKLLWCLWLLVLMSLPLQVWGPPYKIGVVGPWTCDPLFSKALPEIAAQLATERINKDPALDLGHSLEYVIFNEDCQASRALSSFISHHQMASGFIGPANPGYCEAASLLGNSWDKGIFSWACVNYELDSKNSHPTFSRTLPSPIRVLLTVMKYFQWAHAGVISSDEDIWVHTAYRVASALRSRGLPVGVVLTTGQDSQSIQKALQQIRQADRIRIIIMCMHSTLIGGETQTHLLEWAHDLQMTDGTYVFVPYDTLLYSLPYKHIPYQVLRNNPKLREAYDAVLTITVESQEKTFYQAFEEAAARGEIPEKLESDQVSPLFGTIYNSIYFIAQAMNNAMKENGWASAASLVQHSRNMQFYGFNQLIRTDSNGNGISEYVILDTNWKEWELHSTYTVDMETELLRFGETPIHFPGGRPPRADAQCWFADGKICQGGINPTFALMVCLALLIALLSINGFAYFIRHRINKIQLIKGPNRILLTLEDVTFINPHFGSKRGSHASVSFQITSEVQSGRSPRLSFSSGSLTPATCENSNIAIYEGDWVWLKKFPSGNFGDIKSVESSASDIFEMMKDLRHENINPLVGFFYDSGVFAIVTEFCSRRSLEDILMNQDVKLDWMFKSSLLLDLIKGMKYLHHREFAHGRLKSRNCVVDGRFVLKVTDYGFNDILETLRLSQEEPSAEELLWTAPELLRAPRGSRLCSFAGDVYSFAIIMQEVMVRGTPFCMMDLPAKEIIERIKKSPPVYRPVVPPEHAPPECLQLMKQCWAEAAEQRPTFDEIFNQFKTFNKGKKTNIIDSMLRMLEQYSSNLEDLIQERTEELEIEKQKTEKLLTQMLPPSVAESLKKGCTVEPEGFDLVTLYFSDIVGFTTISAMSEPIEVVDLLNDLYTLFDAIIGSHDVYKVETIGDAYMVASGLPKRNGMRHAAEIANMSLDILSSVGTFKMRHMPEVPVRIRIGLHSGPVVAGVVGLTMPRYCLFGDTVNTASRMESTGLPYRIHVSHSTVTILRTLGEGYEVELRGRTELKGKGTEETFWLVGKKGFTKPLPVPPPVGKDGQVGHGLQPVEIAAFQRRKAEKQLVRNKP

Target ORF information:

RefSeq Version	XM_006071580.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis guanylate cyclase 2F, retinal (GUCY2F), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006071580.2

ORF Insert Sequence:

ATGTTCTTGG GACCCTGGCC TTTCTCCCAC CTTATGCTCT GGTTCGCGAC TCTGGGAAGA 
CAGCGAGGAC AACATGGCCT TGCATCTTTC AAGCTCCTGT GGTGTTTGTG GCTCTTGGTC 
CTTATGTCTC TCCCACTGCA GGTGTGGGGG CCACCCTACA AGATAGGGGT GGTTGGCCCT 
TGGACTTGTG ATCCATTGTT CTCAAAGGCC CTGCCTGAGA TTGCTGCTCA GTTAGCCACT 
GAGCGGATCA ACAAGGACCC AGCCTTGGAC CTGGGTCACT CTCTTGAATA TGTGATTTTC 
AATGAAGACT GCCAGGCTTC CAGGGCTCTC TCCAGTTTCA TTTCCCACCA CCAGATGGCC 
TCAGGGTTTA TTGGACCTGC CAACCCTGGC TACTGCGAGG CAGCCTCGCT CCTGGGAAAC 
AGCTGGGACA AAGGGATTTT CTCCTGGGCT TGCGTGAATT ATGAATTAGA CAGTAAAAAT 
AGCCACCCGA CTTTTTCTCG GACTCTCCCT TCTCCCATCC GGGTGCTGCT AACTGTCATG 
AAATATTTCC AGTGGGCTCA CGCTGGAGTC ATTTCCTCAG ATGAAGACAT TTGGGTGCAC 
ACAGCCTATC GAGTTGCAAG TGCTCTTCGG AGCCGCGGCC TACCAGTAGG GGTCGTCCTG 
ACCACAGGAC AAGACAGCCA AAGCATCCAG AAAGCTCTCC AGCAGATTCG CCAGGCAGAT 
AGAATTCGCA TAATCATCAT GTGTATGCAT TCGACTTTGA TTGGGGGAGA GACTCAGACG 
CATCTCTTGG AATGGGCTCA CGATCTGCAA ATGACTGATG GCACCTATGT CTTTGTTCCC 
TATGACACCC TGCTCTACAG TTTACCTTAT AAGCATATCC CCTACCAGGT TTTAAGGAAC 
AACCCAAAAC TCCGGGAAGC CTATGATGCT GTGTTGACCA TCACAGTAGA GTCCCAAGAA 
AAGACCTTCT ATCAAGCCTT TGAGGAGGCA GCAGCTAGAG GTGAAATTCC TGAGAAGCTG 
GAGTCAGATC AAGTTTCGCC GTTGTTTGGA ACCATCTACA ATTCAATTTA CTTTATCGCA 
CAAGCCATGA ATAATGCTAT GAAAGAAAAT GGATGGGCTA GTGCTGCCAG CCTGGTTCAG 
CATTCCAGAA ACATGCAGTT CTATGGATTC AACCAGTTGA TAAGGACAGA TTCAAATGGA 
AATGGAATTT CAGAATATGT AATCCTGGAC ACCAACTGGA AAGAATGGGA ACTCCATAGT 
ACCTACACTG TGGACATGGA AACGGAGCTG CTACGATTCG GAGAGACCCC TATTCACTTT 
CCTGGTGGCA GGCCCCCTCG AGCAGATGCA CAATGCTGGT TTGCAGATGG GAAGATCTGC 
CAAGGAGGCA TCAACCCTAC CTTTGCTTTG ATGGTCTGCC TTGCTTTGCT TATAGCCCTG 
CTGTCTATTA ATGGATTTGC TTACTTTATA AGACATCGTA TAAATAAAAT CCAGTTGATT 
AAAGGACCCA ACAGAATTCT ACTGACTTTG GAGGATGTGA CATTTATCAA TCCCCACTTT 
GGCAGTAAGA GAGGAAGTCA CGCCAGTGTA AGTTTCCAGA TCACGTCGGA GGTCCAGAGT 
GGAAGGTCCC CAAGACTCTC CTTTTCTTCA GGGAGTCTAA CTCCAGCTAC CTGTGAAAAC 
TCCAACATAG CGATTTATGA GGGTGATTGG GTATGGCTGA AAAAATTCCC CTCAGGAAAT 
TTTGGAGACA TTAAATCCGT CGAATCAAGT GCAAGTGACA TATTTGAAAT GATGAAGGAC 
CTGCGTCATG AGAATATTAA CCCTTTGGTG GGCTTCTTCT ATGATTCGGG GGTATTTGCC 
ATTGTGACAG AATTCTGTTC CCGAAGGAGC CTGGAAGACA TACTGATGAA TCAGGATGTG 
AAACTAGATT GGATGTTTAA ATCATCACTC CTGCTGGATC TCATCAAGGG CATGAAGTAC 
TTACACCACA GAGAGTTTGC TCATGGGAGG CTGAAGTCTC GGAACTGTGT GGTGGATGGG 
CGTTTTGTAC TCAAAGTGAC AGATTATGGC TTTAATGACA TCTTGGAAAC GCTAAGACTC 
TCCCAAGAGG AACCTTCTGC AGAAGAGTTG CTGTGGACAG CCCCTGAACT GTTGAGAGCC 
CCGAGGGGCA GCAGGTTATG TTCTTTTGCA GGAGACGTCT ATAGCTTTGC CATCATCATG 
CAGGAAGTGA TGGTCCGGGG CACCCCATTC TGCATGATGG ATCTGCCTGC CAAAGAAATC 
ATAGAAAGAA TTAAGAAGTC TCCTCCAGTG TACAGACCAG TGGTTCCTCC TGAGCATGCA 
CCCCCAGAAT GTCTCCAGCT GATGAAGCAG TGCTGGGCTG AGGCTGCAGA ACAACGACCA 
ACATTTGATG AAATATTTAA CCAGTTTAAA ACTTTCAATA AAGGGAAGAA GACTAATATT 
ATTGACTCTA TGCTTCGGAT GCTGGAGCAA TATTCTAGCA ACTTGGAAGA TTTGATCCAG 
GAGCGAACTG AAGAGCTGGA AATTGAAAAA CAGAAAACGG AAAAGCTCCT AACACAGATG 
CTACCACCAT CAGTTGCTGA ATCTCTCAAA AAGGGCTGCA CAGTCGAACC GGAGGGCTTT 
GATTTGGTCA CCTTGTACTT CAGCGACATT GTGGGCTTCA CTACCATCTC AGCCATGAGT 
GAGCCCATTG AGGTGGTGGA TCTTCTCAAT GACCTGTACA CCCTCTTTGA TGCCATCATT 
GGCAGTCATG ATGTCTACAA GGTAGAGACT ATTGGAGATG CTTACATGGT GGCCTCAGGC 
CTCCCAAAGA GGAATGGCAT GAGGCATGCA GCCGAGATTG CAAACATGTC CCTAGACATC 
CTCAGCTCTG TGGGTACCTT CAAGATGCGG CACATGCCAG AGGTGCCGGT CCGGATTCGA 
ATAGGCCTGC ACTCAGGGCC GGTTGTTGCT GGAGTGGTGG GCCTCACTAT GCCCAGATAC 
TGCTTGTTTG GAGATACTGT GAACACAGCT TCTCGGATGG AATCAACAGG GTTACCTTAT 
CGCATTCATG TCAGTCATAG CACAGTGACA ATTCTTCGAA CCCTGGGTGA AGGCTATGAA 
GTGGAGCTTC GAGGAAGAAC AGAGCTCAAG GGCAAAGGCA CAGAAGAGAC CTTCTGGCTA 
GTTGGGAAAA AGGGCTTCAC GAAGCCCCTT CCTGTGCCCC CACCAGTGGG CAAAGATGGG 
CAAGTGGGCC ATGGCCTTCA ACCAGTAGAG ATTGCAGCCT TCCAAAGAAG AAAAGCAGAA 
AAGCAGCTGG TGAGAAACAA GCCATAA