PHKB gene cDNA ORF clone, Bubalus bubalis(water buffalo)

mRNA	Protein	Name
XM_006062751.1	XP_006062813.1	phosphorylase b kinase regulatory subunit beta isoform X2
XM_006062753.1	XP_006062815.1	phosphorylase b kinase regulatory subunit beta isoform X4
XM_006062754.1	XP_006062816.1	phosphorylase b kinase regulatory subunit beta isoform X5
XM_025269796.1	XP_025125581.1	phosphorylase b kinase regulatory subunit beta isoform X1
XM_025269797.1	XP_025125582.1	phosphorylase b kinase regulatory subunit beta isoform X1
XM_025269801.1	XP_025125586.1	phosphorylase b kinase regulatory subunit beta isoform X4
XM_006062752.1	XP_006062814.1	phosphorylase b kinase regulatory subunit beta isoform X3
XM_025269800.1	XP_025125585.1	phosphorylase b kinase regulatory subunit beta isoform X3
XM_025269805.1	XP_025125590.1	phosphorylase b kinase regulatory subunit beta isoform X7
XM_025269804.1	XP_025125589.1	phosphorylase b kinase regulatory subunit beta isoform X6
XM_025269798.1	XP_025125583.1	phosphorylase b kinase regulatory subunit beta isoform X1
XM_025269799.1	XP_025125584.1	phosphorylase b kinase regulatory subunit beta isoform X2
XM_006062750.1	XP_006062812.1	phosphorylase b kinase regulatory subunit beta isoform X1
XM_025269802.1	XP_025125587.1	phosphorylase b kinase regulatory subunit beta isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu18797	XM_006062750.1 Latest version!	Bubalus bubalis phosphorylase kinase, beta (PHKB), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBu18798	XM_006062751.1 Latest version!	Bubalus bubalis phosphorylase kinase, beta (PHKB), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBu18799	XM_006062752.1 Latest version!	Bubalus bubalis phosphorylase kinase, beta (PHKB), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBu18800	XM_006062753.1 Latest version!	Bubalus bubalis phosphorylase kinase, beta (PHKB), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBu18800	XM_006062754.1 Latest version!	Bubalus bubalis phosphorylase kinase, beta (PHKB), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBu53301	XM_025269797.1 Latest version!	Bubalus bubalis phosphorylase kinase regulatory subunit beta (PHKB), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBu53301	XM_025269796.1 Latest version!	Bubalus bubalis phosphorylase kinase regulatory subunit beta (PHKB), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBu53301	XM_025269798.1 Latest version!	Bubalus bubalis phosphorylase kinase regulatory subunit beta (PHKB), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBu53302	XM_025269799.1 Latest version!	Bubalus bubalis phosphorylase kinase regulatory subunit beta (PHKB), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBu53303	XM_025269800.1 Latest version!	Bubalus bubalis phosphorylase kinase regulatory subunit beta (PHKB), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBu53304	XM_025269801.1 Latest version!	Bubalus bubalis phosphorylase kinase regulatory subunit beta (PHKB), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBu53305	XM_025269802.1 Latest version!	Bubalus bubalis phosphorylase kinase regulatory subunit beta (PHKB), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBu53306	XM_025269804.1 Latest version!	Bubalus bubalis phosphorylase kinase regulatory subunit beta (PHKB), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBu53307	XM_025269805.1 Latest version!	Bubalus bubalis phosphorylase kinase regulatory subunit beta (PHKB), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

PHKB ( XM_006062750.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006062812.1 Bubalus bubalis phosphorylase kinase, beta (PHKB), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu18797
Clone ID Related Accession (Same CDS sequence)	XM_006062750.1
Accession Version	XM_006062750.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2637bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	phosphorylase b kinase regulatory subunit beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785058.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGC TCATGGCCGA AGTAAGCTGG AAGGTCTTGG AGCGAAGAGC CCGGACCAAG 
CGCTCAGCTT TCTTAAGGTC TGGCTCAGTT TATGAACCTC TGAAAAGCAT TAATCTTCCG 
AGACCTGATG AAACTCTGTG GGATAAACTG GATCACTATT ACAGAATTGT CAAGTCAACA 
TTGCTGCTGT ATCAAAGTCC AACCACAGGT CTCTTTCCCA CTAAAACATG TGGTAATGAT 
CAGAAGGCCA AGGTTCATGA CAGTCTGTAC TGTGCTGCTG GTGCCTGGGC TTTGGCTCTT 
GCATACAGGC GCATTGATGA TGACAAGGGA AGGACCCATG AGCTGGAGCA CTCGGCTATA 
AAATGTATGA GAGGAATTCT CTACTGCTAC ATGCGTCAGG CCGATAAGGT CCAGCAATTT 
AAGCAGGATC CACGCCCAAC AACATGTCTT CACTCCATTT TCAATGTACA TACAGGAGAT 
GAGATCCTCT CCTATGAGGA ATATGGTCAT CTTCAGATAA ATGCAGTGTC CCTCTATCTC 
CTTTACCTTG TCGAAATGAT TTCCTCAGGA CTCCAGATTA TCTACAACAC TGATGAGGTC 
TCTTTTATTC AGAACCTTGT ATTTTGTGTA GAAAGAGTTT ACCGGGTGCC TGACTTCGGT 
GTCTGGGAAA GAGGAAGCAA ATATAATAAT GGCAGCACAG AGCTGCATTC GAGCTCTGTT 
GGCTTAGCAA AAGCAGCTCT AGAAGCAATT AATGGATTCA ACCTCTTTGG CAACCAGGGC 
TGTTCTTGGT CAGTTATATT TGTGGATCTC GATGCCCACA ATCGCAACCG GCAAACTTTG 
TGCTCGTTGT TACCCAGAGA ATCAAGATCT CATAACACAG ATGCTGCCCT GCTCCCCTGC 
ATCAGTTATC CTGCGTTTGC CTTGGATGAT GAAGTTCTCT TTAGCCAGAC GCTTGATAAA 
GTGATTAGAA AATTAAAAGG AAAATATGGA TTTAAGCGTT TCTTGAGAGA TGGATATAGA 
ACATCATTGG AAGATCCCAA TAGACGCTAC TACAAACCAG CTGAAATTAA GCTGTTTGAT 
GGCATTGAAT GTGAATTTCC CATATTTTTC CTTTACATGA TGATTGATGG TGTTTTTAGA 
GGCAATCCCA AGCAAGTAAA AGAGTATCAG GATCTTCTGA CTCCAGTACT TCATCACACC 
ACAGAAGGTT ATCCTGTCAT ACCAAAGTAC TATTATGTGC CAGCTGATTT TGTAGAGTAT 
GAAAAAAGAA ACCCTGGTAG TCAGAAGCGA TTCCCTAGCA ACTGTGGCCG TGATGGAAAA 
CTGTTTCTTT GGGGACAAGC CCTTTATGTC ATTGCAAAAC TCCTGGCTGA TGAATTAATT 
AGTCCTAAAG ACATTGATCC TGTTCAGCGT TTTGTCCCTC TACAGAATCA ACGAAATGTG 
AGCATGAGGT TTTCCAATCA GGGTCCACTG GAAAATGATT TGGTTGTTTA TGTAGCACTC 
ATAGCAGAAA GCCAACGCCT TCAAGTTTTC CTCAACACAT ATGGTATTCA GACTCAAACT 
CCTCAACAGG TGGAACCCAT TCAGATCTGG GCCCAGCAGG AGCTGGTGAA AGCTTATTTC 
CATCTGGGTA TCAATGAAAA ATTAGGACTC TGCGGAAGGC CAGATAGACC TATTGGCTGC 
CTTGGTACAT CAAAGATTTA TCGCATTCTA GGAAAGACTG TGGTTTGTTA CCCTATCATC 
TTTGACCTTA GTGATTTCTA CATGTCCCAA GATGTTTTGC TGCTGATAGA TGACATAAAG 
AATGCACTCC AGTTTATTAA GCAATACTGG AAAATGCATG GACGTCCACT TTTCCTTGTT 
CTCATCCGGG AAGACAATAT AAGAGGTAGC CGATTCAATC CCATATTAGA TATGCTGGCA 
GCATTTAAGA AAGGAATGGT TGGAGGAGTC AAAGTTCATG TTGATCGTCT ACAGACACTA 
ATATCTGGAG CTGTGGTTGA ACAACTTGAC TTCTTACGAA TCAGTGAAAT AGAAGAGCTT 
CCAGAATTTA AGAGTTTTGA GGAATTAGAA GTTCCCAAAC ATTCAAAAGT CAAACGACAA 
AGCAGCACCT CCAATGCTCC CGAACAGGAA CAGAAGCCAG ATGTTACCGT GACTGAATGG 
AGAAGCAAAC CCACCCATGA AATTCTTCAA AAACTGAATG ACTGTAGTTG TCTGGCTAGC 
CAAGCCATCC TACTGGGTAT ACTGCTCAAA AGAGAAGGCC CCAACTTCAT CACAAAGGAC 
GGTACTGTTT CTGATCACAT TGAGAGAGTC TATAGAAGAG CTGGCAGCAA AAAACTTTGG 
TCGGTGGTGC GCCATGCAGC AAGTCTTTTA AGTAAACTAG TGGACAGCCT GGCCCCATCC 
ATTACTAATG TTTTAGTGCA GGGCAAACAG GTAACTCTGG GTGCCTTTGG GCATGAAGAA 
GAAGTTATCT CTAATCCTTT GTCTCCGGGA GTGATTAAGA ACATCATCTA TTACAAGTGT 
AATACCCATG ATGAGAGGGA GGCAGTAATT CAGCAAGAAC TGGTCATCCA CATTGGCTGG 
ATCATCTCCA ATAACCCAGA ATTATTCAGT GGCATGCTGA AAATACGAAT TGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006062812.1
CDS	46..2682
Translation	MAGLMAEVSWKVLERRARTKRSAFLRSGSVYEPLKSINLPRPDETLWDKLDHYYRIVKSTLLLYQSPTTGLFPTKTCGNDQKAKVHDSLYCAAGAWALALAYRRIDDDKGRTHELEHSAIKCMRGILYCYMRQADKVQQFKQDPRPTTCLHSIFNVHTGDEILSYEEYGHLQINAVSLYLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERVYRVPDFGVWERGSKYNNGSTELHSSSVGLAKAALEAINGFNLFGNQGCSWSVIFVDLDAHNRNRQTLCSLLPRESRSHNTDAALLPCISYPAFALDDEVLFSQTLDKVIRKLKGKYGFKRFLRDGYRTSLEDPNRRYYKPAEIKLFDGIECEFPIFFLYMMIDGVFRGNPKQVKEYQDLLTPVLHHTTEGYPVIPKYYYVPADFVEYEKRNPGSQKRFPSNCGRDGKLFLWGQALYVIAKLLADELISPKDIDPVQRFVPLQNQRNVSMRFSNQGPLENDLVVYVALIAESQRLQVFLNTYGIQTQTPQQVEPIQIWAQQELVKAYFHLGINEKLGLCGRPDRPIGCLGTSKIYRILGKTVVCYPIIFDLSDFYMSQDVLLLIDDIKNALQFIKQYWKMHGRPLFLVLIREDNIRGSRFNPILDMLAAFKKGMVGGVKVHVDRLQTLISGAVVEQLDFLRISEIEELPEFKSFEELEVPKHSKVKRQSSTSNAPEQEQKPDVTVTEWRSKPTHEILQKLNDCSCLASQAILLGILLKREGPNFITKDGTVSDHIERVYRRAGSKKLWSVVRHAASLLSKLVDSLAPSITNVLVQGKQVTLGAFGHEEEVISNPLSPGVIKNIIYYKCNTHDEREAVIQQELVIHIGWIISNNPELFSGMLKIRIG

Target ORF information:

RefSeq Version	XM_006062750.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis phosphorylase kinase, beta (PHKB), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006062750.1

ORF Insert Sequence:

ATGGCGGGGC TCATGGCCGA AGTAAGCTGG AAGGTCTTGG AGCGAAGAGC CCGGACCAAG 
CGCTCAGCTT TCTTAAGGTC TGGCTCAGTT TATGAACCTC TGAAAAGCAT TAATCTTCCG 
AGACCTGATG AAACTCTGTG GGATAAACTG GATCACTATT ACAGAATTGT CAAGTCAACA 
TTGCTGCTGT ATCAAAGTCC AACCACAGGT CTCTTTCCCA CTAAAACATG TGGTAATGAT 
CAGAAGGCCA AGGTTCATGA CAGTCTGTAC TGTGCTGCTG GTGCCTGGGC TTTGGCTCTT 
GCATACAGGC GCATTGATGA TGACAAGGGA AGGACCCATG AGCTGGAGCA CTCGGCTATA 
AAATGTATGA GAGGAATTCT CTACTGCTAC ATGCGTCAGG CCGATAAGGT CCAGCAATTT 
AAGCAGGATC CACGCCCAAC AACATGTCTT CACTCCATTT TCAATGTACA TACAGGAGAT 
GAGATCCTCT CCTATGAGGA ATATGGTCAT CTTCAGATAA ATGCAGTGTC CCTCTATCTC 
CTTTACCTTG TCGAAATGAT TTCCTCAGGA CTCCAGATTA TCTACAACAC TGATGAGGTC 
TCTTTTATTC AGAACCTTGT ATTTTGTGTA GAAAGAGTTT ACCGGGTGCC TGACTTCGGT 
GTCTGGGAAA GAGGAAGCAA ATATAATAAT GGCAGCACAG AGCTGCATTC GAGCTCTGTT 
GGCTTAGCAA AAGCAGCTCT AGAAGCAATT AATGGATTCA ACCTCTTTGG CAACCAGGGC 
TGTTCTTGGT CAGTTATATT TGTGGATCTC GATGCCCACA ATCGCAACCG GCAAACTTTG 
TGCTCGTTGT TACCCAGAGA ATCAAGATCT CATAACACAG ATGCTGCCCT GCTCCCCTGC 
ATCAGTTATC CTGCGTTTGC CTTGGATGAT GAAGTTCTCT TTAGCCAGAC GCTTGATAAA 
GTGATTAGAA AATTAAAAGG AAAATATGGA TTTAAGCGTT TCTTGAGAGA TGGATATAGA 
ACATCATTGG AAGATCCCAA TAGACGCTAC TACAAACCAG CTGAAATTAA GCTGTTTGAT 
GGCATTGAAT GTGAATTTCC CATATTTTTC CTTTACATGA TGATTGATGG TGTTTTTAGA 
GGCAATCCCA AGCAAGTAAA AGAGTATCAG GATCTTCTGA CTCCAGTACT TCATCACACC 
ACAGAAGGTT ATCCTGTCAT ACCAAAGTAC TATTATGTGC CAGCTGATTT TGTAGAGTAT 
GAAAAAAGAA ACCCTGGTAG TCAGAAGCGA TTCCCTAGCA ACTGTGGCCG TGATGGAAAA 
CTGTTTCTTT GGGGACAAGC CCTTTATGTC ATTGCAAAAC TCCTGGCTGA TGAATTAATT 
AGTCCTAAAG ACATTGATCC TGTTCAGCGT TTTGTCCCTC TACAGAATCA ACGAAATGTG 
AGCATGAGGT TTTCCAATCA GGGTCCACTG GAAAATGATT TGGTTGTTTA TGTAGCACTC 
ATAGCAGAAA GCCAACGCCT TCAAGTTTTC CTCAACACAT ATGGTATTCA GACTCAAACT 
CCTCAACAGG TGGAACCCAT TCAGATCTGG GCCCAGCAGG AGCTGGTGAA AGCTTATTTC 
CATCTGGGTA TCAATGAAAA ATTAGGACTC TGCGGAAGGC CAGATAGACC TATTGGCTGC 
CTTGGTACAT CAAAGATTTA TCGCATTCTA GGAAAGACTG TGGTTTGTTA CCCTATCATC 
TTTGACCTTA GTGATTTCTA CATGTCCCAA GATGTTTTGC TGCTGATAGA TGACATAAAG 
AATGCACTCC AGTTTATTAA GCAATACTGG AAAATGCATG GACGTCCACT TTTCCTTGTT 
CTCATCCGGG AAGACAATAT AAGAGGTAGC CGATTCAATC CCATATTAGA TATGCTGGCA 
GCATTTAAGA AAGGAATGGT TGGAGGAGTC AAAGTTCATG TTGATCGTCT ACAGACACTA 
ATATCTGGAG CTGTGGTTGA ACAACTTGAC TTCTTACGAA TCAGTGAAAT AGAAGAGCTT 
CCAGAATTTA AGAGTTTTGA GGAATTAGAA GTTCCCAAAC ATTCAAAAGT CAAACGACAA 
AGCAGCACCT CCAATGCTCC CGAACAGGAA CAGAAGCCAG ATGTTACCGT GACTGAATGG 
AGAAGCAAAC CCACCCATGA AATTCTTCAA AAACTGAATG ACTGTAGTTG TCTGGCTAGC 
CAAGCCATCC TACTGGGTAT ACTGCTCAAA AGAGAAGGCC CCAACTTCAT CACAAAGGAC 
GGTACTGTTT CTGATCACAT TGAGAGAGTC TATAGAAGAG CTGGCAGCAA AAAACTTTGG 
TCGGTGGTGC GCCATGCAGC AAGTCTTTTA AGTAAACTAG TGGACAGCCT GGCCCCATCC 
ATTACTAATG TTTTAGTGCA GGGCAAACAG GTAACTCTGG GTGCCTTTGG GCATGAAGAA 
GAAGTTATCT CTAATCCTTT GTCTCCGGGA GTGATTAAGA ACATCATCTA TTACAAGTGT 
AATACCCATG ATGAGAGGGA GGCAGTAATT CAGCAAGAAC TGGTCATCCA CATTGGCTGG 
ATCATCTCCA ATAACCCAGA ATTATTCAGT GGCATGCTGA AAATACGAAT TGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKB ( XM_006062751.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006062813.1 Bubalus bubalis phosphorylase kinase, beta (PHKB), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu18798
Clone ID Related Accession (Same CDS sequence)	XM_006062751.1
Accession Version	XM_006062751.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2622bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	phosphorylase b kinase regulatory subunit beta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785058.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGC TCATGGCCGA AGTAAGCTGG AAGGTCTTGG AGCGAAGAGC CCGGACCAAG 
CGCTCAGGCT CAGTTTATGA ACCTCTGAAA AGCATTAATC TTCCGAGACC TGATGAAACT 
CTGTGGGATA AACTGGATCA CTATTACAGA ATTGTCAAGT CAACATTGCT GCTGTATCAA 
AGTCCAACCA CAGGTCTCTT TCCCACTAAA ACATGTGGTA ATGATCAGAA GGCCAAGGTT 
CATGACAGTC TGTACTGTGC TGCTGGTGCC TGGGCTTTGG CTCTTGCATA CAGGCGCATT 
GATGATGACA AGGGAAGGAC CCATGAGCTG GAGCACTCGG CTATAAAATG TATGAGAGGA 
ATTCTCTACT GCTACATGCG TCAGGCCGAT AAGGTCCAGC AATTTAAGCA GGATCCACGC 
CCAACAACAT GTCTTCACTC CATTTTCAAT GTACATACAG GAGATGAGAT CCTCTCCTAT 
GAGGAATATG GTCATCTTCA GATAAATGCA GTGTCCCTCT ATCTCCTTTA CCTTGTCGAA 
ATGATTTCCT CAGGACTCCA GATTATCTAC AACACTGATG AGGTCTCTTT TATTCAGAAC 
CTTGTATTTT GTGTAGAAAG AGTTTACCGG GTGCCTGACT TCGGTGTCTG GGAAAGAGGA 
AGCAAATATA ATAATGGCAG CACAGAGCTG CATTCGAGCT CTGTTGGCTT AGCAAAAGCA 
GCTCTAGAAG CAATTAATGG ATTCAACCTC TTTGGCAACC AGGGCTGTTC TTGGTCAGTT 
ATATTTGTGG ATCTCGATGC CCACAATCGC AACCGGCAAA CTTTGTGCTC GTTGTTACCC 
AGAGAATCAA GATCTCATAA CACAGATGCT GCCCTGCTCC CCTGCATCAG TTATCCTGCG 
TTTGCCTTGG ATGATGAAGT TCTCTTTAGC CAGACGCTTG ATAAAGTGAT TAGAAAATTA 
AAAGGAAAAT ATGGATTTAA GCGTTTCTTG AGAGATGGAT ATAGAACATC ATTGGAAGAT 
CCCAATAGAC GCTACTACAA ACCAGCTGAA ATTAAGCTGT TTGATGGCAT TGAATGTGAA 
TTTCCCATAT TTTTCCTTTA CATGATGATT GATGGTGTTT TTAGAGGCAA TCCCAAGCAA 
GTAAAAGAGT ATCAGGATCT TCTGACTCCA GTACTTCATC ACACCACAGA AGGTTATCCT 
GTCATACCAA AGTACTATTA TGTGCCAGCT GATTTTGTAG AGTATGAAAA AAGAAACCCT 
GGTAGTCAGA AGCGATTCCC TAGCAACTGT GGCCGTGATG GAAAACTGTT TCTTTGGGGA 
CAAGCCCTTT ATGTCATTGC AAAACTCCTG GCTGATGAAT TAATTAGTCC TAAAGACATT 
GATCCTGTTC AGCGTTTTGT CCCTCTACAG AATCAACGAA ATGTGAGCAT GAGGTTTTCC 
AATCAGGGTC CACTGGAAAA TGATTTGGTT GTTTATGTAG CACTCATAGC AGAAAGCCAA 
CGCCTTCAAG TTTTCCTCAA CACATATGGT ATTCAGACTC AAACTCCTCA ACAGGTGGAA 
CCCATTCAGA TCTGGGCCCA GCAGGAGCTG GTGAAAGCTT ATTTCCATCT GGGTATCAAT 
GAAAAATTAG GACTCTGCGG AAGGCCAGAT AGACCTATTG GCTGCCTTGG TACATCAAAG 
ATTTATCGCA TTCTAGGAAA GACTGTGGTT TGTTACCCTA TCATCTTTGA CCTTAGTGAT 
TTCTACATGT CCCAAGATGT TTTGCTGCTG ATAGATGACA TAAAGAATGC ACTCCAGTTT 
ATTAAGCAAT ACTGGAAAAT GCATGGACGT CCACTTTTCC TTGTTCTCAT CCGGGAAGAC 
AATATAAGAG GTAGCCGATT CAATCCCATA TTAGATATGC TGGCAGCATT TAAGAAAGGA 
ATGGTTGGAG GAGTCAAAGT TCATGTTGAT CGTCTACAGA CACTAATATC TGGAGCTGTG 
GTTGAACAAC TTGACTTCTT ACGAATCAGT GAAATAGAAG AGCTTCCAGA ATTTAAGAGT 
TTTGAGGAAT TAGAAGTTCC CAAACATTCA AAAGTCAAAC GACAAAGCAG CACCTCCAAT 
GCTCCCGAAC AGGAACAGAA GCCAGATGTT ACCGTGACTG AATGGAGAAG CAAACCCACC 
CATGAAATTC TTCAAAAACT GAATGACTGT AGTTGTCTGG CTAGCCAAGC CATCCTACTG 
GGTATACTGC TCAAAAGAGA AGGCCCCAAC TTCATCACAA AGGACGGTAC TGTTTCTGAT 
CACATTGAGA GAGTCTATAG AAGAGCTGGC AGCAAAAAAC TTTGGTCGGT GGTGCGCCAT 
GCAGCAAGTC TTTTAAGTAA ACTAGTGGAC AGCCTGGCCC CATCCATTAC TAATGTTTTA 
GTGCAGGGCA AACAGGTAAC TCTGGGTGCC TTTGGGCATG AAGAAGAAGT TATCTCTAAT 
CCTTTGTCTC CGGGAGTGAT TAAGAACATC ATCTATTACA AGTGTAATAC CCATGATGAG 
AGGGAGGCAG TAATTCAGCA AGAACTGGTC ATCCACATTG GCTGGATCAT CTCCAATAAC 
CCAGAATTAT TCAGTGGCAT GCTGAAAATA CGAATTGGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006062813.1
CDS	46..2667
Translation	MAGLMAEVSWKVLERRARTKRSGSVYEPLKSINLPRPDETLWDKLDHYYRIVKSTLLLYQSPTTGLFPTKTCGNDQKAKVHDSLYCAAGAWALALAYRRIDDDKGRTHELEHSAIKCMRGILYCYMRQADKVQQFKQDPRPTTCLHSIFNVHTGDEILSYEEYGHLQINAVSLYLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERVYRVPDFGVWERGSKYNNGSTELHSSSVGLAKAALEAINGFNLFGNQGCSWSVIFVDLDAHNRNRQTLCSLLPRESRSHNTDAALLPCISYPAFALDDEVLFSQTLDKVIRKLKGKYGFKRFLRDGYRTSLEDPNRRYYKPAEIKLFDGIECEFPIFFLYMMIDGVFRGNPKQVKEYQDLLTPVLHHTTEGYPVIPKYYYVPADFVEYEKRNPGSQKRFPSNCGRDGKLFLWGQALYVIAKLLADELISPKDIDPVQRFVPLQNQRNVSMRFSNQGPLENDLVVYVALIAESQRLQVFLNTYGIQTQTPQQVEPIQIWAQQELVKAYFHLGINEKLGLCGRPDRPIGCLGTSKIYRILGKTVVCYPIIFDLSDFYMSQDVLLLIDDIKNALQFIKQYWKMHGRPLFLVLIREDNIRGSRFNPILDMLAAFKKGMVGGVKVHVDRLQTLISGAVVEQLDFLRISEIEELPEFKSFEELEVPKHSKVKRQSSTSNAPEQEQKPDVTVTEWRSKPTHEILQKLNDCSCLASQAILLGILLKREGPNFITKDGTVSDHIERVYRRAGSKKLWSVVRHAASLLSKLVDSLAPSITNVLVQGKQVTLGAFGHEEEVISNPLSPGVIKNIIYYKCNTHDEREAVIQQELVIHIGWIISNNPELFSGMLKIRIG

Target ORF information:

RefSeq Version	XM_006062751.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis phosphorylase kinase, beta (PHKB), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006062751.1

ORF Insert Sequence:

ATGGCGGGGC TCATGGCCGA AGTAAGCTGG AAGGTCTTGG AGCGAAGAGC CCGGACCAAG 
CGCTCAGGCT CAGTTTATGA ACCTCTGAAA AGCATTAATC TTCCGAGACC TGATGAAACT 
CTGTGGGATA AACTGGATCA CTATTACAGA ATTGTCAAGT CAACATTGCT GCTGTATCAA 
AGTCCAACCA CAGGTCTCTT TCCCACTAAA ACATGTGGTA ATGATCAGAA GGCCAAGGTT 
CATGACAGTC TGTACTGTGC TGCTGGTGCC TGGGCTTTGG CTCTTGCATA CAGGCGCATT 
GATGATGACA AGGGAAGGAC CCATGAGCTG GAGCACTCGG CTATAAAATG TATGAGAGGA 
ATTCTCTACT GCTACATGCG TCAGGCCGAT AAGGTCCAGC AATTTAAGCA GGATCCACGC 
CCAACAACAT GTCTTCACTC CATTTTCAAT GTACATACAG GAGATGAGAT CCTCTCCTAT 
GAGGAATATG GTCATCTTCA GATAAATGCA GTGTCCCTCT ATCTCCTTTA CCTTGTCGAA 
ATGATTTCCT CAGGACTCCA GATTATCTAC AACACTGATG AGGTCTCTTT TATTCAGAAC 
CTTGTATTTT GTGTAGAAAG AGTTTACCGG GTGCCTGACT TCGGTGTCTG GGAAAGAGGA 
AGCAAATATA ATAATGGCAG CACAGAGCTG CATTCGAGCT CTGTTGGCTT AGCAAAAGCA 
GCTCTAGAAG CAATTAATGG ATTCAACCTC TTTGGCAACC AGGGCTGTTC TTGGTCAGTT 
ATATTTGTGG ATCTCGATGC CCACAATCGC AACCGGCAAA CTTTGTGCTC GTTGTTACCC 
AGAGAATCAA GATCTCATAA CACAGATGCT GCCCTGCTCC CCTGCATCAG TTATCCTGCG 
TTTGCCTTGG ATGATGAAGT TCTCTTTAGC CAGACGCTTG ATAAAGTGAT TAGAAAATTA 
AAAGGAAAAT ATGGATTTAA GCGTTTCTTG AGAGATGGAT ATAGAACATC ATTGGAAGAT 
CCCAATAGAC GCTACTACAA ACCAGCTGAA ATTAAGCTGT TTGATGGCAT TGAATGTGAA 
TTTCCCATAT TTTTCCTTTA CATGATGATT GATGGTGTTT TTAGAGGCAA TCCCAAGCAA 
GTAAAAGAGT ATCAGGATCT TCTGACTCCA GTACTTCATC ACACCACAGA AGGTTATCCT 
GTCATACCAA AGTACTATTA TGTGCCAGCT GATTTTGTAG AGTATGAAAA AAGAAACCCT 
GGTAGTCAGA AGCGATTCCC TAGCAACTGT GGCCGTGATG GAAAACTGTT TCTTTGGGGA 
CAAGCCCTTT ATGTCATTGC AAAACTCCTG GCTGATGAAT TAATTAGTCC TAAAGACATT 
GATCCTGTTC AGCGTTTTGT CCCTCTACAG AATCAACGAA ATGTGAGCAT GAGGTTTTCC 
AATCAGGGTC CACTGGAAAA TGATTTGGTT GTTTATGTAG CACTCATAGC AGAAAGCCAA 
CGCCTTCAAG TTTTCCTCAA CACATATGGT ATTCAGACTC AAACTCCTCA ACAGGTGGAA 
CCCATTCAGA TCTGGGCCCA GCAGGAGCTG GTGAAAGCTT ATTTCCATCT GGGTATCAAT 
GAAAAATTAG GACTCTGCGG AAGGCCAGAT AGACCTATTG GCTGCCTTGG TACATCAAAG 
ATTTATCGCA TTCTAGGAAA GACTGTGGTT TGTTACCCTA TCATCTTTGA CCTTAGTGAT 
TTCTACATGT CCCAAGATGT TTTGCTGCTG ATAGATGACA TAAAGAATGC ACTCCAGTTT 
ATTAAGCAAT ACTGGAAAAT GCATGGACGT CCACTTTTCC TTGTTCTCAT CCGGGAAGAC 
AATATAAGAG GTAGCCGATT CAATCCCATA TTAGATATGC TGGCAGCATT TAAGAAAGGA 
ATGGTTGGAG GAGTCAAAGT TCATGTTGAT CGTCTACAGA CACTAATATC TGGAGCTGTG 
GTTGAACAAC TTGACTTCTT ACGAATCAGT GAAATAGAAG AGCTTCCAGA ATTTAAGAGT 
TTTGAGGAAT TAGAAGTTCC CAAACATTCA AAAGTCAAAC GACAAAGCAG CACCTCCAAT 
GCTCCCGAAC AGGAACAGAA GCCAGATGTT ACCGTGACTG AATGGAGAAG CAAACCCACC 
CATGAAATTC TTCAAAAACT GAATGACTGT AGTTGTCTGG CTAGCCAAGC CATCCTACTG 
GGTATACTGC TCAAAAGAGA AGGCCCCAAC TTCATCACAA AGGACGGTAC TGTTTCTGAT 
CACATTGAGA GAGTCTATAG AAGAGCTGGC AGCAAAAAAC TTTGGTCGGT GGTGCGCCAT 
GCAGCAAGTC TTTTAAGTAA ACTAGTGGAC AGCCTGGCCC CATCCATTAC TAATGTTTTA 
GTGCAGGGCA AACAGGTAAC TCTGGGTGCC TTTGGGCATG AAGAAGAAGT TATCTCTAAT 
CCTTTGTCTC CGGGAGTGAT TAAGAACATC ATCTATTACA AGTGTAATAC CCATGATGAG 
AGGGAGGCAG TAATTCAGCA AGAACTGGTC ATCCACATTG GCTGGATCAT CTCCAATAAC 
CCAGAATTAT TCAGTGGCAT GCTGAAAATA CGAATTGGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKB ( XM_006062752.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006062814.1 Bubalus bubalis phosphorylase kinase, beta (PHKB), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu18799
Clone ID Related Accession (Same CDS sequence)	XM_006062752.1
Accession Version	XM_006062752.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2622bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	phosphorylase b kinase regulatory subunit beta isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785058.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGC TCATGGCCGA AGTAAGCTGG AAGGTCTTGG AGCGAAGAGC CCGGACCAAG 
CGCTCAGGCT CAGTTTATGA ACCTCTGAAA AGCATTAATC TTCCGAGACC TGATGAAACT 
CTGTGGGATA AACTGGATCA CTATTACAGA ATTGTCAAGT CAACATTGCT GCTGTATCAA 
AGTCCAACCA CAGGTCTCTT TCCCACTAAA ACATGTGGTA ATGATCAGAA GGCCAAGGTT 
CATGACAGTC TGTACTGTGC TGCTGGTGCC TGGGCTTTGG CTCTTGCATA CAGGCGCATT 
GATGATGACA AGGGAAGGAC CCATGAGCTG GAGCACTCGG CTATAAAATG TATGAGAGGA 
ATTCTCTACT GCTACATGCG TCAGGCCGAT AAGGTCCAGC AATTTAAGCA GGATCCACGC 
CCAACAACAT GTCTTCACTC CATTTTCAAT GTACATACAG GAGATGAGAT CCTCTCCTAT 
GAGGAATATG GTCATCTTCA GATAAATGCA GTGTCCCTCT ATCTCCTTTA CCTTGTCGAA 
ATGATTTCCT CAGGACTCCA GATTATCTAC AACACTGATG AGGTCTCTTT TATTCAGAAC 
CTTGTATTTT GTGTAGAAAG AGTTTACCGG GTGCCTGACT TCGGTGTCTG GGAAAGAGGA 
AGCAAATATA ATAATGGCAG CACAGAGCTG CATTCGAGCT CTGTTGGCTT AGCAAAAGCA 
GCTCTAGAAG CAATTAATGG ATTCAACCTC TTTGGCAACC AGGGCTGTTC TTGGTCAGTT 
ATATTTGTGG ATCTCGATGC CCACAATCGC AACCGGCAAA CTTTGTGCTC GTTGTTACCC 
AGAGAATCAA GATCTCATAA CACAGATGCT GCCCTGCTCC CCTGCATCAG TTATCCTGCG 
TTTGCCTTGG ATGATGAAGT TCTCTTTAGC CAGACGCTTG ATAAAGTGAT TAGAAAATTA 
AAAGGAAAAT ATGGATTTAA GCGTTTCTTG AGAGATGGAT ATAGAACATC ATTGGAAGAT 
CCCAATAGAC GCTACTACAA ACCAGCTGAA ATTAAGCTGT TTGATGGCAT TGAATGTGAA 
TTTCCCATAT TTTTCCTTTA CATGATGATT GATGGTGTTT TTAGAGGCAA TCCCAAGCAA 
GTAAAAGAGT ATCAGGATCT TCTGACTCCA GTACTTCATC ACACCACAGA AGGTTATCCT 
GTCATACCAA AGTACTATTA TGTGCCAGCT GATTTTGTAG AGTATGAAAA AAGAAACCCT 
GGTAGTCAGA AGCGATTCCC TAGCAACTGT GGCCGTGATG GAAAACTGTT TCTTTGGGGA 
CAAGCCCTTT ATGTCATTGC AAAACTCCTG GCTGATGAAT TAATTAGTCC TAAAGACATT 
GATCCTGTTC AGCGTTTTGT CCCTCTACAG AATCAACGAA ATGTGAGCAT GAGGTTTTCC 
AATCAGGGTC CACTGGAAAA TGATTTGGTT GTTTATGTAG CACTCATAGC AGAAAGCCAA 
CGCCTTCAAG TTTTCCTCAA CACATATGGT ATTCAGACTC AAACTCCTCA ACAGGTGGAA 
CCCATTCAGA TCTGGGCCCA GCAGGAGCTG GTGAAAGCTT ATTTCCATCT GGGTATCAAT 
GAAAAATTAG GACTCTGCGG AAGGCCAGAT AGACCTATTG GCTGCCTTGG TACATCAAAG 
ATTTATCGCA TTCTAGGAAA GACTGTGGTT TGTTACCCTA TCATCTTTGA CCTTAGTGAT 
TTCTACATGT CCCAAGATGT TTTGCTGCTG ATAGATGACA TAAAGAATGC ACTCCAGTTT 
ATTAAGCAAT ACTGGAAAAT GCATGGACGT CCACTTTTCC TTGTTCTCAT CCGGGAAGAC 
AATATAAGAG GTAGCCGATT CAATCCCATA TTAGATATGC TGGCAGCATT TAAGAAAGGA 
ATGGTTGGAG GAGTCAAAGT TCATGTTGAT CGTCTACAGA CACTAATATC TGGAGCTGTG 
GTTGAACAAC TTGACTTCTT ACGAATCAGT GAAATAGAAG AGCTTCCAGA ATTTAAGAGT 
TTTGAGGAAT TAGAAGTTCC CAAACATTCA AAAGTCAAAC GACAAAGCAG CACCTCCAAT 
GCTCCCGAAC AGGAACAGAA GCCAGATGTT ACCGTGACTG AATGGAGAAG CAAACCCACC 
CATGAAATTC TTCAAAAACT GAATGACTGT AGTTGTCTGG CTAGCCAAGC CATCCTACTG 
GGTATACTGC TCAAAAGAGA AGGCCCCAAC TTCATCACAA AGGACGGTAC TGTTTCTGAT 
CACATTGAGA GAGTCTATAG AAGAGCTGGC AGCAAAAAAC TTTGGTTGGC GGTACGCTAC 
GGGGCTGCAT TTACGCAGAA ATTTTCTTCC TCTATAGCCC CACACATTAC TACTTTTCTG 
GTACATGGGA AACAGGTAAC TCTGGGTGCC TTTGGGCATG AAGAAGAAGT TATCTCTAAT 
CCTTTGTCTC CGGGAGTGAT TAAGAACATC ATCTATTACA AGTGTAATAC CCATGATGAG 
AGGGAGGCAG TAATTCAGCA AGAACTGGTC ATCCACATTG GCTGGATCAT CTCCAATAAC 
CCAGAATTAT TCAGTGGCAT GCTGAAAATA CGAATTGGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006062814.1
CDS	46..2667
Translation	MAGLMAEVSWKVLERRARTKRSGSVYEPLKSINLPRPDETLWDKLDHYYRIVKSTLLLYQSPTTGLFPTKTCGNDQKAKVHDSLYCAAGAWALALAYRRIDDDKGRTHELEHSAIKCMRGILYCYMRQADKVQQFKQDPRPTTCLHSIFNVHTGDEILSYEEYGHLQINAVSLYLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERVYRVPDFGVWERGSKYNNGSTELHSSSVGLAKAALEAINGFNLFGNQGCSWSVIFVDLDAHNRNRQTLCSLLPRESRSHNTDAALLPCISYPAFALDDEVLFSQTLDKVIRKLKGKYGFKRFLRDGYRTSLEDPNRRYYKPAEIKLFDGIECEFPIFFLYMMIDGVFRGNPKQVKEYQDLLTPVLHHTTEGYPVIPKYYYVPADFVEYEKRNPGSQKRFPSNCGRDGKLFLWGQALYVIAKLLADELISPKDIDPVQRFVPLQNQRNVSMRFSNQGPLENDLVVYVALIAESQRLQVFLNTYGIQTQTPQQVEPIQIWAQQELVKAYFHLGINEKLGLCGRPDRPIGCLGTSKIYRILGKTVVCYPIIFDLSDFYMSQDVLLLIDDIKNALQFIKQYWKMHGRPLFLVLIREDNIRGSRFNPILDMLAAFKKGMVGGVKVHVDRLQTLISGAVVEQLDFLRISEIEELPEFKSFEELEVPKHSKVKRQSSTSNAPEQEQKPDVTVTEWRSKPTHEILQKLNDCSCLASQAILLGILLKREGPNFITKDGTVSDHIERVYRRAGSKKLWLAVRYGAAFTQKFSSSIAPHITTFLVHGKQVTLGAFGHEEEVISNPLSPGVIKNIIYYKCNTHDEREAVIQQELVIHIGWIISNNPELFSGMLKIRIG

Target ORF information:

RefSeq Version	XM_006062752.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis phosphorylase kinase, beta (PHKB), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006062752.1

ORF Insert Sequence:

ATGGCGGGGC TCATGGCCGA AGTAAGCTGG AAGGTCTTGG AGCGAAGAGC CCGGACCAAG 
CGCTCAGGCT CAGTTTATGA ACCTCTGAAA AGCATTAATC TTCCGAGACC TGATGAAACT 
CTGTGGGATA AACTGGATCA CTATTACAGA ATTGTCAAGT CAACATTGCT GCTGTATCAA 
AGTCCAACCA CAGGTCTCTT TCCCACTAAA ACATGTGGTA ATGATCAGAA GGCCAAGGTT 
CATGACAGTC TGTACTGTGC TGCTGGTGCC TGGGCTTTGG CTCTTGCATA CAGGCGCATT 
GATGATGACA AGGGAAGGAC CCATGAGCTG GAGCACTCGG CTATAAAATG TATGAGAGGA 
ATTCTCTACT GCTACATGCG TCAGGCCGAT AAGGTCCAGC AATTTAAGCA GGATCCACGC 
CCAACAACAT GTCTTCACTC CATTTTCAAT GTACATACAG GAGATGAGAT CCTCTCCTAT 
GAGGAATATG GTCATCTTCA GATAAATGCA GTGTCCCTCT ATCTCCTTTA CCTTGTCGAA 
ATGATTTCCT CAGGACTCCA GATTATCTAC AACACTGATG AGGTCTCTTT TATTCAGAAC 
CTTGTATTTT GTGTAGAAAG AGTTTACCGG GTGCCTGACT TCGGTGTCTG GGAAAGAGGA 
AGCAAATATA ATAATGGCAG CACAGAGCTG CATTCGAGCT CTGTTGGCTT AGCAAAAGCA 
GCTCTAGAAG CAATTAATGG ATTCAACCTC TTTGGCAACC AGGGCTGTTC TTGGTCAGTT 
ATATTTGTGG ATCTCGATGC CCACAATCGC AACCGGCAAA CTTTGTGCTC GTTGTTACCC 
AGAGAATCAA GATCTCATAA CACAGATGCT GCCCTGCTCC CCTGCATCAG TTATCCTGCG 
TTTGCCTTGG ATGATGAAGT TCTCTTTAGC CAGACGCTTG ATAAAGTGAT TAGAAAATTA 
AAAGGAAAAT ATGGATTTAA GCGTTTCTTG AGAGATGGAT ATAGAACATC ATTGGAAGAT 
CCCAATAGAC GCTACTACAA ACCAGCTGAA ATTAAGCTGT TTGATGGCAT TGAATGTGAA 
TTTCCCATAT TTTTCCTTTA CATGATGATT GATGGTGTTT TTAGAGGCAA TCCCAAGCAA 
GTAAAAGAGT ATCAGGATCT TCTGACTCCA GTACTTCATC ACACCACAGA AGGTTATCCT 
GTCATACCAA AGTACTATTA TGTGCCAGCT GATTTTGTAG AGTATGAAAA AAGAAACCCT 
GGTAGTCAGA AGCGATTCCC TAGCAACTGT GGCCGTGATG GAAAACTGTT TCTTTGGGGA 
CAAGCCCTTT ATGTCATTGC AAAACTCCTG GCTGATGAAT TAATTAGTCC TAAAGACATT 
GATCCTGTTC AGCGTTTTGT CCCTCTACAG AATCAACGAA ATGTGAGCAT GAGGTTTTCC 
AATCAGGGTC CACTGGAAAA TGATTTGGTT GTTTATGTAG CACTCATAGC AGAAAGCCAA 
CGCCTTCAAG TTTTCCTCAA CACATATGGT ATTCAGACTC AAACTCCTCA ACAGGTGGAA 
CCCATTCAGA TCTGGGCCCA GCAGGAGCTG GTGAAAGCTT ATTTCCATCT GGGTATCAAT 
GAAAAATTAG GACTCTGCGG AAGGCCAGAT AGACCTATTG GCTGCCTTGG TACATCAAAG 
ATTTATCGCA TTCTAGGAAA GACTGTGGTT TGTTACCCTA TCATCTTTGA CCTTAGTGAT 
TTCTACATGT CCCAAGATGT TTTGCTGCTG ATAGATGACA TAAAGAATGC ACTCCAGTTT 
ATTAAGCAAT ACTGGAAAAT GCATGGACGT CCACTTTTCC TTGTTCTCAT CCGGGAAGAC 
AATATAAGAG GTAGCCGATT CAATCCCATA TTAGATATGC TGGCAGCATT TAAGAAAGGA 
ATGGTTGGAG GAGTCAAAGT TCATGTTGAT CGTCTACAGA CACTAATATC TGGAGCTGTG 
GTTGAACAAC TTGACTTCTT ACGAATCAGT GAAATAGAAG AGCTTCCAGA ATTTAAGAGT 
TTTGAGGAAT TAGAAGTTCC CAAACATTCA AAAGTCAAAC GACAAAGCAG CACCTCCAAT 
GCTCCCGAAC AGGAACAGAA GCCAGATGTT ACCGTGACTG AATGGAGAAG CAAACCCACC 
CATGAAATTC TTCAAAAACT GAATGACTGT AGTTGTCTGG CTAGCCAAGC CATCCTACTG 
GGTATACTGC TCAAAAGAGA AGGCCCCAAC TTCATCACAA AGGACGGTAC TGTTTCTGAT 
CACATTGAGA GAGTCTATAG AAGAGCTGGC AGCAAAAAAC TTTGGTTGGC GGTACGCTAC 
GGGGCTGCAT TTACGCAGAA ATTTTCTTCC TCTATAGCCC CACACATTAC TACTTTTCTG 
GTACATGGGA AACAGGTAAC TCTGGGTGCC TTTGGGCATG AAGAAGAAGT TATCTCTAAT 
CCTTTGTCTC CGGGAGTGAT TAAGAACATC ATCTATTACA AGTGTAATAC CCATGATGAG 
AGGGAGGCAG TAATTCAGCA AGAACTGGTC ATCCACATTG GCTGGATCAT CTCCAATAAC 
CCAGAATTAT TCAGTGGCAT GCTGAAAATA CGAATTGGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKB ( XM_006062753.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006062815.1 Bubalus bubalis phosphorylase kinase, beta (PHKB), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu18800
Clone ID Related Accession (Same CDS sequence)	XM_006062753.1 , XM_006062754.1
Accession Version	XM_006062753.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2610bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	phosphorylase b kinase regulatory subunit beta isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785058.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT CACCTGATCC AGTCGTCCCT TCTCCATCAG CTTTCTTAAG GTCTGGCTCA 
GTTTATGAAC CTCTGAAAAG CATTAATCTT CCGAGACCTG ATGAAACTCT GTGGGATAAA 
CTGGATCACT ATTACAGAAT TGTCAAGTCA ACATTGCTGC TGTATCAAAG TCCAACCACA 
GGTCTCTTTC CCACTAAAAC ATGTGGTAAT GATCAGAAGG CCAAGGTTCA TGACAGTCTG 
TACTGTGCTG CTGGTGCCTG GGCTTTGGCT CTTGCATACA GGCGCATTGA TGATGACAAG 
GGAAGGACCC ATGAGCTGGA GCACTCGGCT ATAAAATGTA TGAGAGGAAT TCTCTACTGC 
TACATGCGTC AGGCCGATAA GGTCCAGCAA TTTAAGCAGG ATCCACGCCC AACAACATGT 
CTTCACTCCA TTTTCAATGT ACATACAGGA GATGAGATCC TCTCCTATGA GGAATATGGT 
CATCTTCAGA TAAATGCAGT GTCCCTCTAT CTCCTTTACC TTGTCGAAAT GATTTCCTCA 
GGACTCCAGA TTATCTACAA CACTGATGAG GTCTCTTTTA TTCAGAACCT TGTATTTTGT 
GTAGAAAGAG TTTACCGGGT GCCTGACTTC GGTGTCTGGG AAAGAGGAAG CAAATATAAT 
AATGGCAGCA CAGAGCTGCA TTCGAGCTCT GTTGGCTTAG CAAAAGCAGC TCTAGAAGCA 
ATTAATGGAT TCAACCTCTT TGGCAACCAG GGCTGTTCTT GGTCAGTTAT ATTTGTGGAT 
CTCGATGCCC ACAATCGCAA CCGGCAAACT TTGTGCTCGT TGTTACCCAG AGAATCAAGA 
TCTCATAACA CAGATGCTGC CCTGCTCCCC TGCATCAGTT ATCCTGCGTT TGCCTTGGAT 
GATGAAGTTC TCTTTAGCCA GACGCTTGAT AAAGTGATTA GAAAATTAAA AGGAAAATAT 
GGATTTAAGC GTTTCTTGAG AGATGGATAT AGAACATCAT TGGAAGATCC CAATAGACGC 
TACTACAAAC CAGCTGAAAT TAAGCTGTTT GATGGCATTG AATGTGAATT TCCCATATTT 
TTCCTTTACA TGATGATTGA TGGTGTTTTT AGAGGCAATC CCAAGCAAGT AAAAGAGTAT 
CAGGATCTTC TGACTCCAGT ACTTCATCAC ACCACAGAAG GTTATCCTGT CATACCAAAG 
TACTATTATG TGCCAGCTGA TTTTGTAGAG TATGAAAAAA GAAACCCTGG TAGTCAGAAG 
CGATTCCCTA GCAACTGTGG CCGTGATGGA AAACTGTTTC TTTGGGGACA AGCCCTTTAT 
GTCATTGCAA AACTCCTGGC TGATGAATTA ATTAGTCCTA AAGACATTGA TCCTGTTCAG 
CGTTTTGTCC CTCTACAGAA TCAACGAAAT GTGAGCATGA GGTTTTCCAA TCAGGGTCCA 
CTGGAAAATG ATTTGGTTGT TTATGTAGCA CTCATAGCAG AAAGCCAACG CCTTCAAGTT 
TTCCTCAACA CATATGGTAT TCAGACTCAA ACTCCTCAAC AGGTGGAACC CATTCAGATC 
TGGGCCCAGC AGGAGCTGGT GAAAGCTTAT TTCCATCTGG GTATCAATGA AAAATTAGGA 
CTCTGCGGAA GGCCAGATAG ACCTATTGGC TGCCTTGGTA CATCAAAGAT TTATCGCATT 
CTAGGAAAGA CTGTGGTTTG TTACCCTATC ATCTTTGACC TTAGTGATTT CTACATGTCC 
CAAGATGTTT TGCTGCTGAT AGATGACATA AAGAATGCAC TCCAGTTTAT TAAGCAATAC 
TGGAAAATGC ATGGACGTCC ACTTTTCCTT GTTCTCATCC GGGAAGACAA TATAAGAGGT 
AGCCGATTCA ATCCCATATT AGATATGCTG GCAGCATTTA AGAAAGGAAT GGTTGGAGGA 
GTCAAAGTTC ATGTTGATCG TCTACAGACA CTAATATCTG GAGCTGTGGT TGAACAACTT 
GACTTCTTAC GAATCAGTGA AATAGAAGAG CTTCCAGAAT TTAAGAGTTT TGAGGAATTA 
GAAGTTCCCA AACATTCAAA AGTCAAACGA CAAAGCAGCA CCTCCAATGC TCCCGAACAG 
GAACAGAAGC CAGATGTTAC CGTGACTGAA TGGAGAAGCA AACCCACCCA TGAAATTCTT 
CAAAAACTGA ATGACTGTAG TTGTCTGGCT AGCCAAGCCA TCCTACTGGG TATACTGCTC 
AAAAGAGAAG GCCCCAACTT CATCACAAAG GACGGTACTG TTTCTGATCA CATTGAGAGA 
GTCTATAGAA GAGCTGGCAG CAAAAAACTT TGGTCGGTGG TGCGCCATGC AGCAAGTCTT 
TTAAGTAAAC TAGTGGACAG CCTGGCCCCA TCCATTACTA ATGTTTTAGT GCAGGGCAAA 
CAGGTAACTC TGGGTGCCTT TGGGCATGAA GAAGAAGTTA TCTCTAATCC TTTGTCTCCG 
GGAGTGATTA AGAACATCAT CTATTACAAG TGTAATACCC ATGATGAGAG GGAGGCAGTA 
ATTCAGCAAG AACTGGTCAT CCACATTGGC TGGATCATCT CCAATAACCC AGAATTATTC 
AGTGGCATGC TGAAAATACG AATTGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006062815.1
CDS	142..2751
Translation	MASSPDPVVPSPSAFLRSGSVYEPLKSINLPRPDETLWDKLDHYYRIVKSTLLLYQSPTTGLFPTKTCGNDQKAKVHDSLYCAAGAWALALAYRRIDDDKGRTHELEHSAIKCMRGILYCYMRQADKVQQFKQDPRPTTCLHSIFNVHTGDEILSYEEYGHLQINAVSLYLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERVYRVPDFGVWERGSKYNNGSTELHSSSVGLAKAALEAINGFNLFGNQGCSWSVIFVDLDAHNRNRQTLCSLLPRESRSHNTDAALLPCISYPAFALDDEVLFSQTLDKVIRKLKGKYGFKRFLRDGYRTSLEDPNRRYYKPAEIKLFDGIECEFPIFFLYMMIDGVFRGNPKQVKEYQDLLTPVLHHTTEGYPVIPKYYYVPADFVEYEKRNPGSQKRFPSNCGRDGKLFLWGQALYVIAKLLADELISPKDIDPVQRFVPLQNQRNVSMRFSNQGPLENDLVVYVALIAESQRLQVFLNTYGIQTQTPQQVEPIQIWAQQELVKAYFHLGINEKLGLCGRPDRPIGCLGTSKIYRILGKTVVCYPIIFDLSDFYMSQDVLLLIDDIKNALQFIKQYWKMHGRPLFLVLIREDNIRGSRFNPILDMLAAFKKGMVGGVKVHVDRLQTLISGAVVEQLDFLRISEIEELPEFKSFEELEVPKHSKVKRQSSTSNAPEQEQKPDVTVTEWRSKPTHEILQKLNDCSCLASQAILLGILLKREGPNFITKDGTVSDHIERVYRRAGSKKLWSVVRHAASLLSKLVDSLAPSITNVLVQGKQVTLGAFGHEEEVISNPLSPGVIKNIIYYKCNTHDEREAVIQQELVIHIGWIISNNPELFSGMLKIRIG

Target ORF information:

RefSeq Version	XM_006062753.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis phosphorylase kinase, beta (PHKB), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006062753.1

ORF Insert Sequence:

ATGGCCAGCT CACCTGATCC AGTCGTCCCT TCTCCATCAG CTTTCTTAAG GTCTGGCTCA 
GTTTATGAAC CTCTGAAAAG CATTAATCTT CCGAGACCTG ATGAAACTCT GTGGGATAAA 
CTGGATCACT ATTACAGAAT TGTCAAGTCA ACATTGCTGC TGTATCAAAG TCCAACCACA 
GGTCTCTTTC CCACTAAAAC ATGTGGTAAT GATCAGAAGG CCAAGGTTCA TGACAGTCTG 
TACTGTGCTG CTGGTGCCTG GGCTTTGGCT CTTGCATACA GGCGCATTGA TGATGACAAG 
GGAAGGACCC ATGAGCTGGA GCACTCGGCT ATAAAATGTA TGAGAGGAAT TCTCTACTGC 
TACATGCGTC AGGCCGATAA GGTCCAGCAA TTTAAGCAGG ATCCACGCCC AACAACATGT 
CTTCACTCCA TTTTCAATGT ACATACAGGA GATGAGATCC TCTCCTATGA GGAATATGGT 
CATCTTCAGA TAAATGCAGT GTCCCTCTAT CTCCTTTACC TTGTCGAAAT GATTTCCTCA 
GGACTCCAGA TTATCTACAA CACTGATGAG GTCTCTTTTA TTCAGAACCT TGTATTTTGT 
GTAGAAAGAG TTTACCGGGT GCCTGACTTC GGTGTCTGGG AAAGAGGAAG CAAATATAAT 
AATGGCAGCA CAGAGCTGCA TTCGAGCTCT GTTGGCTTAG CAAAAGCAGC TCTAGAAGCA 
ATTAATGGAT TCAACCTCTT TGGCAACCAG GGCTGTTCTT GGTCAGTTAT ATTTGTGGAT 
CTCGATGCCC ACAATCGCAA CCGGCAAACT TTGTGCTCGT TGTTACCCAG AGAATCAAGA 
TCTCATAACA CAGATGCTGC CCTGCTCCCC TGCATCAGTT ATCCTGCGTT TGCCTTGGAT 
GATGAAGTTC TCTTTAGCCA GACGCTTGAT AAAGTGATTA GAAAATTAAA AGGAAAATAT 
GGATTTAAGC GTTTCTTGAG AGATGGATAT AGAACATCAT TGGAAGATCC CAATAGACGC 
TACTACAAAC CAGCTGAAAT TAAGCTGTTT GATGGCATTG AATGTGAATT TCCCATATTT 
TTCCTTTACA TGATGATTGA TGGTGTTTTT AGAGGCAATC CCAAGCAAGT AAAAGAGTAT 
CAGGATCTTC TGACTCCAGT ACTTCATCAC ACCACAGAAG GTTATCCTGT CATACCAAAG 
TACTATTATG TGCCAGCTGA TTTTGTAGAG TATGAAAAAA GAAACCCTGG TAGTCAGAAG 
CGATTCCCTA GCAACTGTGG CCGTGATGGA AAACTGTTTC TTTGGGGACA AGCCCTTTAT 
GTCATTGCAA AACTCCTGGC TGATGAATTA ATTAGTCCTA AAGACATTGA TCCTGTTCAG 
CGTTTTGTCC CTCTACAGAA TCAACGAAAT GTGAGCATGA GGTTTTCCAA TCAGGGTCCA 
CTGGAAAATG ATTTGGTTGT TTATGTAGCA CTCATAGCAG AAAGCCAACG CCTTCAAGTT 
TTCCTCAACA CATATGGTAT TCAGACTCAA ACTCCTCAAC AGGTGGAACC CATTCAGATC 
TGGGCCCAGC AGGAGCTGGT GAAAGCTTAT TTCCATCTGG GTATCAATGA AAAATTAGGA 
CTCTGCGGAA GGCCAGATAG ACCTATTGGC TGCCTTGGTA CATCAAAGAT TTATCGCATT 
CTAGGAAAGA CTGTGGTTTG TTACCCTATC ATCTTTGACC TTAGTGATTT CTACATGTCC 
CAAGATGTTT TGCTGCTGAT AGATGACATA AAGAATGCAC TCCAGTTTAT TAAGCAATAC 
TGGAAAATGC ATGGACGTCC ACTTTTCCTT GTTCTCATCC GGGAAGACAA TATAAGAGGT 
AGCCGATTCA ATCCCATATT AGATATGCTG GCAGCATTTA AGAAAGGAAT GGTTGGAGGA 
GTCAAAGTTC ATGTTGATCG TCTACAGACA CTAATATCTG GAGCTGTGGT TGAACAACTT 
GACTTCTTAC GAATCAGTGA AATAGAAGAG CTTCCAGAAT TTAAGAGTTT TGAGGAATTA 
GAAGTTCCCA AACATTCAAA AGTCAAACGA CAAAGCAGCA CCTCCAATGC TCCCGAACAG 
GAACAGAAGC CAGATGTTAC CGTGACTGAA TGGAGAAGCA AACCCACCCA TGAAATTCTT 
CAAAAACTGA ATGACTGTAG TTGTCTGGCT AGCCAAGCCA TCCTACTGGG TATACTGCTC 
AAAAGAGAAG GCCCCAACTT CATCACAAAG GACGGTACTG TTTCTGATCA CATTGAGAGA 
GTCTATAGAA GAGCTGGCAG CAAAAAACTT TGGTCGGTGG TGCGCCATGC AGCAAGTCTT 
TTAAGTAAAC TAGTGGACAG CCTGGCCCCA TCCATTACTA ATGTTTTAGT GCAGGGCAAA 
CAGGTAACTC TGGGTGCCTT TGGGCATGAA GAAGAAGTTA TCTCTAATCC TTTGTCTCCG 
GGAGTGATTA AGAACATCAT CTATTACAAG TGTAATACCC ATGATGAGAG GGAGGCAGTA 
ATTCAGCAAG AACTGGTCAT CCACATTGGC TGGATCATCT CCAATAACCC AGAATTATTC 
AGTGGCATGC TGAAAATACG AATTGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKB ( XM_006062754.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006062816.1 Bubalus bubalis phosphorylase kinase, beta (PHKB), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBu18800
Clone ID Related Accession (Same CDS sequence)	XM_006062753.1 , XM_006062754.1
Accession Version	XM_006062754.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2610bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	phosphorylase b kinase regulatory subunit beta isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785058.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT CACCTGATCC AGTCGTCCCT TCTCCATCAG CTTTCTTAAG GTCTGGCTCA 
GTTTATGAAC CTCTGAAAAG CATTAATCTT CCGAGACCTG ATGAAACTCT GTGGGATAAA 
CTGGATCACT ATTACAGAAT TGTCAAGTCA ACATTGCTGC TGTATCAAAG TCCAACCACA 
GGTCTCTTTC CCACTAAAAC ATGTGGTAAT GATCAGAAGG CCAAGGTTCA TGACAGTCTG 
TACTGTGCTG CTGGTGCCTG GGCTTTGGCT CTTGCATACA GGCGCATTGA TGATGACAAG 
GGAAGGACCC ATGAGCTGGA GCACTCGGCT ATAAAATGTA TGAGAGGAAT TCTCTACTGC 
TACATGCGTC AGGCCGATAA GGTCCAGCAA TTTAAGCAGG ATCCACGCCC AACAACATGT 
CTTCACTCCA TTTTCAATGT ACATACAGGA GATGAGATCC TCTCCTATGA GGAATATGGT 
CATCTTCAGA TAAATGCAGT GTCCCTCTAT CTCCTTTACC TTGTCGAAAT GATTTCCTCA 
GGACTCCAGA TTATCTACAA CACTGATGAG GTCTCTTTTA TTCAGAACCT TGTATTTTGT 
GTAGAAAGAG TTTACCGGGT GCCTGACTTC GGTGTCTGGG AAAGAGGAAG CAAATATAAT 
AATGGCAGCA CAGAGCTGCA TTCGAGCTCT GTTGGCTTAG CAAAAGCAGC TCTAGAAGCA 
ATTAATGGAT TCAACCTCTT TGGCAACCAG GGCTGTTCTT GGTCAGTTAT ATTTGTGGAT 
CTCGATGCCC ACAATCGCAA CCGGCAAACT TTGTGCTCGT TGTTACCCAG AGAATCAAGA 
TCTCATAACA CAGATGCTGC CCTGCTCCCC TGCATCAGTT ATCCTGCGTT TGCCTTGGAT 
GATGAAGTTC TCTTTAGCCA GACGCTTGAT AAAGTGATTA GAAAATTAAA AGGAAAATAT 
GGATTTAAGC GTTTCTTGAG AGATGGATAT AGAACATCAT TGGAAGATCC CAATAGACGC 
TACTACAAAC CAGCTGAAAT TAAGCTGTTT GATGGCATTG AATGTGAATT TCCCATATTT 
TTCCTTTACA TGATGATTGA TGGTGTTTTT AGAGGCAATC CCAAGCAAGT AAAAGAGTAT 
CAGGATCTTC TGACTCCAGT ACTTCATCAC ACCACAGAAG GTTATCCTGT CATACCAAAG 
TACTATTATG TGCCAGCTGA TTTTGTAGAG TATGAAAAAA GAAACCCTGG TAGTCAGAAG 
CGATTCCCTA GCAACTGTGG CCGTGATGGA AAACTGTTTC TTTGGGGACA AGCCCTTTAT 
GTCATTGCAA AACTCCTGGC TGATGAATTA ATTAGTCCTA AAGACATTGA TCCTGTTCAG 
CGTTTTGTCC CTCTACAGAA TCAACGAAAT GTGAGCATGA GGTTTTCCAA TCAGGGTCCA 
CTGGAAAATG ATTTGGTTGT TTATGTAGCA CTCATAGCAG AAAGCCAACG CCTTCAAGTT 
TTCCTCAACA CATATGGTAT TCAGACTCAA ACTCCTCAAC AGGTGGAACC CATTCAGATC 
TGGGCCCAGC AGGAGCTGGT GAAAGCTTAT TTCCATCTGG GTATCAATGA AAAATTAGGA 
CTCTGCGGAA GGCCAGATAG ACCTATTGGC TGCCTTGGTA CATCAAAGAT TTATCGCATT 
CTAGGAAAGA CTGTGGTTTG TTACCCTATC ATCTTTGACC TTAGTGATTT CTACATGTCC 
CAAGATGTTT TGCTGCTGAT AGATGACATA AAGAATGCAC TCCAGTTTAT TAAGCAATAC 
TGGAAAATGC ATGGACGTCC ACTTTTCCTT GTTCTCATCC GGGAAGACAA TATAAGAGGT 
AGCCGATTCA ATCCCATATT AGATATGCTG GCAGCATTTA AGAAAGGAAT GGTTGGAGGA 
GTCAAAGTTC ATGTTGATCG TCTACAGACA CTAATATCTG GAGCTGTGGT TGAACAACTT 
GACTTCTTAC GAATCAGTGA AATAGAAGAG CTTCCAGAAT TTAAGAGTTT TGAGGAATTA 
GAAGTTCCCA AACATTCAAA AGTCAAACGA CAAAGCAGCA CCTCCAATGC TCCCGAACAG 
GAACAGAAGC CAGATGTTAC CGTGACTGAA TGGAGAAGCA AACCCACCCA TGAAATTCTT 
CAAAAACTGA ATGACTGTAG TTGTCTGGCT AGCCAAGCCA TCCTACTGGG TATACTGCTC 
AAAAGAGAAG GCCCCAACTT CATCACAAAG GACGGTACTG TTTCTGATCA CATTGAGAGA 
GTCTATAGAA GAGCTGGCAG CAAAAAACTT TGGTCGGTGG TGCGCCATGC AGCAAGTCTT 
TTAAGTAAAC TAGTGGACAG CCTGGCCCCA TCCATTACTA ATGTTTTAGT GCAGGGCAAA 
CAGGTAACTC TGGGTGCCTT TGGGCATGAA GAAGAAGTTA TCTCTAATCC TTTGTCTCCG 
GGAGTGATTA AGAACATCAT CTATTACAAG TGTAATACCC ATGATGAGAG GGAGGCAGTA 
ATTCAGCAAG AACTGGTCAT CCACATTGGC TGGATCATCT CCAATAACCC AGAATTATTC 
AGTGGCATGC TGAAAATACG AATTGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006062816.1
CDS	81..2690
Translation	MASSPDPVVPSPSAFLRSGSVYEPLKSINLPRPDETLWDKLDHYYRIVKSTLLLYQSPTTGLFPTKTCGNDQKAKVHDSLYCAAGAWALALAYRRIDDDKGRTHELEHSAIKCMRGILYCYMRQADKVQQFKQDPRPTTCLHSIFNVHTGDEILSYEEYGHLQINAVSLYLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERVYRVPDFGVWERGSKYNNGSTELHSSSVGLAKAALEAINGFNLFGNQGCSWSVIFVDLDAHNRNRQTLCSLLPRESRSHNTDAALLPCISYPAFALDDEVLFSQTLDKVIRKLKGKYGFKRFLRDGYRTSLEDPNRRYYKPAEIKLFDGIECEFPIFFLYMMIDGVFRGNPKQVKEYQDLLTPVLHHTTEGYPVIPKYYYVPADFVEYEKRNPGSQKRFPSNCGRDGKLFLWGQALYVIAKLLADELISPKDIDPVQRFVPLQNQRNVSMRFSNQGPLENDLVVYVALIAESQRLQVFLNTYGIQTQTPQQVEPIQIWAQQELVKAYFHLGINEKLGLCGRPDRPIGCLGTSKIYRILGKTVVCYPIIFDLSDFYMSQDVLLLIDDIKNALQFIKQYWKMHGRPLFLVLIREDNIRGSRFNPILDMLAAFKKGMVGGVKVHVDRLQTLISGAVVEQLDFLRISEIEELPEFKSFEELEVPKHSKVKRQSSTSNAPEQEQKPDVTVTEWRSKPTHEILQKLNDCSCLASQAILLGILLKREGPNFITKDGTVSDHIERVYRRAGSKKLWSVVRHAASLLSKLVDSLAPSITNVLVQGKQVTLGAFGHEEEVISNPLSPGVIKNIIYYKCNTHDEREAVIQQELVIHIGWIISNNPELFSGMLKIRIG

Target ORF information:

RefSeq Version	XM_006062754.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis phosphorylase kinase, beta (PHKB), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006062754.1

ORF Insert Sequence:

ATGGCCAGCT CACCTGATCC AGTCGTCCCT TCTCCATCAG CTTTCTTAAG GTCTGGCTCA 
GTTTATGAAC CTCTGAAAAG CATTAATCTT CCGAGACCTG ATGAAACTCT GTGGGATAAA 
CTGGATCACT ATTACAGAAT TGTCAAGTCA ACATTGCTGC TGTATCAAAG TCCAACCACA 
GGTCTCTTTC CCACTAAAAC ATGTGGTAAT GATCAGAAGG CCAAGGTTCA TGACAGTCTG 
TACTGTGCTG CTGGTGCCTG GGCTTTGGCT CTTGCATACA GGCGCATTGA TGATGACAAG 
GGAAGGACCC ATGAGCTGGA GCACTCGGCT ATAAAATGTA TGAGAGGAAT TCTCTACTGC 
TACATGCGTC AGGCCGATAA GGTCCAGCAA TTTAAGCAGG ATCCACGCCC AACAACATGT 
CTTCACTCCA TTTTCAATGT ACATACAGGA GATGAGATCC TCTCCTATGA GGAATATGGT 
CATCTTCAGA TAAATGCAGT GTCCCTCTAT CTCCTTTACC TTGTCGAAAT GATTTCCTCA 
GGACTCCAGA TTATCTACAA CACTGATGAG GTCTCTTTTA TTCAGAACCT TGTATTTTGT 
GTAGAAAGAG TTTACCGGGT GCCTGACTTC GGTGTCTGGG AAAGAGGAAG CAAATATAAT 
AATGGCAGCA CAGAGCTGCA TTCGAGCTCT GTTGGCTTAG CAAAAGCAGC TCTAGAAGCA 
ATTAATGGAT TCAACCTCTT TGGCAACCAG GGCTGTTCTT GGTCAGTTAT ATTTGTGGAT 
CTCGATGCCC ACAATCGCAA CCGGCAAACT TTGTGCTCGT TGTTACCCAG AGAATCAAGA 
TCTCATAACA CAGATGCTGC CCTGCTCCCC TGCATCAGTT ATCCTGCGTT TGCCTTGGAT 
GATGAAGTTC TCTTTAGCCA GACGCTTGAT AAAGTGATTA GAAAATTAAA AGGAAAATAT 
GGATTTAAGC GTTTCTTGAG AGATGGATAT AGAACATCAT TGGAAGATCC CAATAGACGC 
TACTACAAAC CAGCTGAAAT TAAGCTGTTT GATGGCATTG AATGTGAATT TCCCATATTT 
TTCCTTTACA TGATGATTGA TGGTGTTTTT AGAGGCAATC CCAAGCAAGT AAAAGAGTAT 
CAGGATCTTC TGACTCCAGT ACTTCATCAC ACCACAGAAG GTTATCCTGT CATACCAAAG 
TACTATTATG TGCCAGCTGA TTTTGTAGAG TATGAAAAAA GAAACCCTGG TAGTCAGAAG 
CGATTCCCTA GCAACTGTGG CCGTGATGGA AAACTGTTTC TTTGGGGACA AGCCCTTTAT 
GTCATTGCAA AACTCCTGGC TGATGAATTA ATTAGTCCTA AAGACATTGA TCCTGTTCAG 
CGTTTTGTCC CTCTACAGAA TCAACGAAAT GTGAGCATGA GGTTTTCCAA TCAGGGTCCA 
CTGGAAAATG ATTTGGTTGT TTATGTAGCA CTCATAGCAG AAAGCCAACG CCTTCAAGTT 
TTCCTCAACA CATATGGTAT TCAGACTCAA ACTCCTCAAC AGGTGGAACC CATTCAGATC 
TGGGCCCAGC AGGAGCTGGT GAAAGCTTAT TTCCATCTGG GTATCAATGA AAAATTAGGA 
CTCTGCGGAA GGCCAGATAG ACCTATTGGC TGCCTTGGTA CATCAAAGAT TTATCGCATT 
CTAGGAAAGA CTGTGGTTTG TTACCCTATC ATCTTTGACC TTAGTGATTT CTACATGTCC 
CAAGATGTTT TGCTGCTGAT AGATGACATA AAGAATGCAC TCCAGTTTAT TAAGCAATAC 
TGGAAAATGC ATGGACGTCC ACTTTTCCTT GTTCTCATCC GGGAAGACAA TATAAGAGGT 
AGCCGATTCA ATCCCATATT AGATATGCTG GCAGCATTTA AGAAAGGAAT GGTTGGAGGA 
GTCAAAGTTC ATGTTGATCG TCTACAGACA CTAATATCTG GAGCTGTGGT TGAACAACTT 
GACTTCTTAC GAATCAGTGA AATAGAAGAG CTTCCAGAAT TTAAGAGTTT TGAGGAATTA 
GAAGTTCCCA AACATTCAAA AGTCAAACGA CAAAGCAGCA CCTCCAATGC TCCCGAACAG 
GAACAGAAGC CAGATGTTAC CGTGACTGAA TGGAGAAGCA AACCCACCCA TGAAATTCTT 
CAAAAACTGA ATGACTGTAG TTGTCTGGCT AGCCAAGCCA TCCTACTGGG TATACTGCTC 
AAAAGAGAAG GCCCCAACTT CATCACAAAG GACGGTACTG TTTCTGATCA CATTGAGAGA 
GTCTATAGAA GAGCTGGCAG CAAAAAACTT TGGTCGGTGG TGCGCCATGC AGCAAGTCTT 
TTAAGTAAAC TAGTGGACAG CCTGGCCCCA TCCATTACTA ATGTTTTAGT GCAGGGCAAA 
CAGGTAACTC TGGGTGCCTT TGGGCATGAA GAAGAAGTTA TCTCTAATCC TTTGTCTCCG 
GGAGTGATTA AGAACATCAT CTATTACAAG TGTAATACCC ATGATGAGAG GGAGGCAGTA 
ATTCAGCAAG AACTGGTCAT CCACATTGGC TGGATCATCT CCAATAACCC AGAATTATTC 
AGTGGCATGC TGAAAATACG AATTGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKB ( XM_025269796.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025125581.1 Bubalus bubalis phosphorylase kinase regulatory subunit beta (PHKB), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu53301
Clone ID Related Accession (Same CDS sequence)	XM_025269796.1 , XM_025269797.1 , XM_025269798.1
Accession Version	XM_025269796.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	phosphorylase b kinase regulatory subunit beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037562.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT CACCTGATCC AGTCGTCCCT TCTCCATCAG CTTTCTTAAG GTCTGATGAG 
AAGAACTGGC TCAGAGAGTG CCCAAGTGTA GAAAGCTGTG GACATAGCTG TGACTGTAGC 
CGAGGCTCAG TTTATGAACC TCTGAAAAGC ATTAATCTTC CGAGACCTGA TGAAACTCTG 
TGGGATAAAC TGGATCACTA TTACAGAATT GTCAAGTCAA CATTGCTGCT GTATCAAAGT 
CCAACCACAG GTCTCTTTCC CACTAAAACA TGTGGTAATG ATCAGAAGGC CAAGGTTCAT 
GACAGTCTGT ACTGTGCTGC TGGTGCCTGG GCTTTGGCTC TTGCATACAG GCGCATTGAT 
GATGACAAGG GAAGGACCCA TGAGCTGGAG CACTCGGCTA TAAAATGTAT GAGAGGAATT 
CTCTACTGCT ACATGCGTCA GGCCGATAAG GTCCAGCAAT TTAAGCAGGA TCCACGCCCA 
ACAACATGTC TTCACTCCAT TTTCAATGTA CATACAGGAG ATGAGATCCT CTCCTATGAG 
GAATATGGTC ATCTTCAGAT AAATGCAGTG TCCCTCTATC TCCTTTACCT TGTCGAAATG 
ATTTCCTCAG GACTCCAGAT TATCTACAAC ACTGATGAGG TCTCTTTTAT TCAGAACCTT 
GTATTTTGTG TAGAAAGAGT TTACCGGGTG CCTGACTTCG GTGTCTGGGA AAGAGGAAGC 
AAATATAATA ATGGCAGCAC AGAGCTGCAT TCGAGCTCTG TTGGCTTAGC AAAAGCAGCT 
CTAGAAGCAA TTAATGGATT CAACCTCTTT GGCAACCAGG GCTGTTCTTG GTCAGTTATA 
TTTGTGGATC TCGATGCCCA CAATCGCAAC CGGCAAACTT TGTGCTCGTT GTTACCCAGA 
GAATCAAGAT CTCATAACAC AGATGCTGCC CTGCTCCCCT GCATCAGTTA TCCTGCGTTT 
GCCTTGGATG ATGAAGTTCT CTTTAGCCAG ACGCTTGATA AAGTGATTAG AAAATTAAAA 
GGAAAATATG GATTTAAGCG TTTCTTGAGA GATGGATATA GAACATCATT GGAAGATCCC 
AATAGACGCT ACTACAAACC AGCTGAAATT AAGCTGTTTG ATGGCATTGA ATGTGAATTT 
CCCATATTTT TCCTTTACAT GATGATTGAT GGTGTTTTTA GAGGCAATCC CAAGCAAGTA 
AAAGAGTATC AGGATCTTCT GACTCCAGTA CTTCATCACA CCACAGAAGG TTATCCTGTC 
ATACCAAAGT ACTATTATGT GCCAGCTGAT TTTGTAGAGT ATGAAAAAAG AAACCCTGGT 
AGTCAGAAGC GATTCCCTAG CAACTGTGGC CGTGATGGAA AACTGTTTCT TTGGGGACAA 
GCCCTTTATG TCATTGCAAA ACTCCTGGCT GATGAATTAA TTAGTCCTAA AGACATTGAT 
CCTGTTCAGC GTTTTGTCCC TCTACAGAAT CAACGAAATG TGAGCATGAG GTTTTCCAAT 
CAGGGTCCAC TGGAAAATGA TTTGGTTGTT TATGTAGCAC TCATAGCAGA AAGCCAACGC 
CTTCAAGTTT TCCTCAACAC ATATGGTATT CAGACTCAAA CTCCTCAACA GGTGGAACCC 
ATTCAGATCT GGGCCCAGCA GGAGCTGGTG AAAGCTTATT TCCATCTGGG TATCAATGAA 
AAATTAGGAC TCTGCGGAAG GCCAGATAGA CCTATTGGCT GCCTTGGTAC ATCAAAGATT 
TATCGCATTC TAGGAAAGAC TGTGGTTTGT TACCCTATCA TCTTTGACCT TAGTGATTTC 
TACATGTCCC AAGATGTTTT GCTGCTGATA GATGACATAA AGAATGCACT CCAGTTTATT 
AAGCAATACT GGAAAATGCA TGGACGTCCA CTTTTCCTTG TTCTCATCCG GGAAGACAAT 
ATAAGAGGTA GCCGATTCAA TCCCATATTA GATATGCTGG CAGCATTTAA GAAAGGAATG 
GTTGGAGGAG TCAAAGTTCA TGTTGATCGT CTACAGACAC TAATATCTGG AGCTGTGGTT 
GAACAACTTG ACTTCTTACG AATCAGTGAA ATAGAAGAGC TTCCAGAATT TAAGAGTTTT 
GAGGAATTAG AAGTTCCCAA ACATTCAAAA GTCAAACGAC AAAGCAGCAC CTCCAATGCT 
CCCGAACAGG AACAGAAGCC AGATGTTACC GTGACTGAAT GGAGAAACAA ACCCACCCAT 
GAAATTCTTC AAAAACTGAA TGACTGTAGT TGTCTGGCTA GCCAAGCCAT CCTACTGGGT 
ATACTGCTCA AAAGAGAAGG CCCCAACTTC ATCACAAAGG ACGGTACTGT TTCTGATCAC 
ATTGAGAGAG TCTATAGAAG AGCTGGCAGC AAAAAACTTT GGTCGGTGGT GCGCCATGCA 
GCAAGTCTTT TAAGTAAACT AGTGGACAGC CTGGCCCCAT CCATTACTAA TGTTTTAGTG 
CAGGGCAAAC AGGTAACTCT GGGTGCCTTT GGGCATGAAG AAGAAGTTAT CTCTAATCCT 
TTGTCTCCGG GAGTGATTAA GAACATCATC TATTACAAGT GTAATACCCA TGATGAGAGG 
GAGGCAGTAA TTCAGCAAGA ACTGGTCATC CACATTGGCT GGATCATCTC CAATAACCCA 
GAATTATTCA GTGGCATGCT GAAAATACGA ATTGGGTGGA TCATCCATGC TATGGAGTAT 
GAACTACAGA TCCGTGGTGG AAACAAGCCA GCCAGAGACT TATATCAGCT GTCACCCAGT 
GAAGTAAAAC AGCTTCTATT GGATATTCTC CAGCCTCAAC AGAATGGAAG ATGTTGGCTG 
AACAGGCGGC AGATCGATGG CTCTTTGAAT AGAACCCCCC CTGGGTTCTA TGACAGAGTA 
TGGCAGATCC TGGAACGCAC ACCCAATGGA ATCATTGTAG CCGGGAGGCA TTTGCCCCAG 
CAACCAACCC TGTCAGATAT GACCATGTAT GAGATGAATT TCTCTCTCCT TGTTGAAGAC 
ATGTTGGGAA ATATTGACCA GCCAAAATAC AGACAGATTG TCGTGGAGTT ACTAATGGTC 
GTATCCATTG TACTGGAAAG AAACCCTGAG CTAGAATTTC AAGACAAAGT AGATCTTGAC 
AAACTGGTGA AAGAAGCATT TCATGAATTT CAAAAAGATG AGAGTCGACT AAAGGAAATT 
GAAAAACAAG ATGACATGAC TTCCTTTTAC AACACTCCCC CTCTGGGAAA AAGAGGAACA 
TGCAGCTATT TGACAAAGGT CGTGATGAAT CTGCTGCTGG AAGGAGAAGT CAAGCCAAGC 
AATGATGACC CGTGTCTGGT TAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025125581.1
CDS	950..4276
Translation	MASSPDPVVPSPSAFLRSDEKNWLRECPSVESCGHSCDCSRGSVYEPLKSINLPRPDETLWDKLDHYYRIVKSTLLLYQSPTTGLFPTKTCGNDQKAKVHDSLYCAAGAWALALAYRRIDDDKGRTHELEHSAIKCMRGILYCYMRQADKVQQFKQDPRPTTCLHSIFNVHTGDEILSYEEYGHLQINAVSLYLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERVYRVPDFGVWERGSKYNNGSTELHSSSVGLAKAALEAINGFNLFGNQGCSWSVIFVDLDAHNRNRQTLCSLLPRESRSHNTDAALLPCISYPAFALDDEVLFSQTLDKVIRKLKGKYGFKRFLRDGYRTSLEDPNRRYYKPAEIKLFDGIECEFPIFFLYMMIDGVFRGNPKQVKEYQDLLTPVLHHTTEGYPVIPKYYYVPADFVEYEKRNPGSQKRFPSNCGRDGKLFLWGQALYVIAKLLADELISPKDIDPVQRFVPLQNQRNVSMRFSNQGPLENDLVVYVALIAESQRLQVFLNTYGIQTQTPQQVEPIQIWAQQELVKAYFHLGINEKLGLCGRPDRPIGCLGTSKIYRILGKTVVCYPIIFDLSDFYMSQDVLLLIDDIKNALQFIKQYWKMHGRPLFLVLIREDNIRGSRFNPILDMLAAFKKGMVGGVKVHVDRLQTLISGAVVEQLDFLRISEIEELPEFKSFEELEVPKHSKVKRQSSTSNAPEQEQKPDVTVTEWRNKPTHEILQKLNDCSCLASQAILLGILLKREGPNFITKDGTVSDHIERVYRRAGSKKLWSVVRHAASLLSKLVDSLAPSITNVLVQGKQVTLGAFGHEEEVISNPLSPGVIKNIIYYKCNTHDEREAVIQQELVIHIGWIISNNPELFSGMLKIRIGWIIHAMEYELQIRGGNKPARDLYQLSPSEVKQLLLDILQPQQNGRCWLNRRQIDGSLNRTPPGFYDRVWQILERTPNGIIVAGRHLPQQPTLSDMTMYEMNFSLLVEDMLGNIDQPKYRQIVVELLMVVSIVLERNPELEFQDKVDLDKLVKEAFHEFQKDESRLKEIEKQDDMTSFYNTPPLGKRGTCSYLTKVVMNLLLEGEVKPSNDDPCLVS

Target ORF information:

RefSeq Version	XM_025269796.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis phosphorylase kinase regulatory subunit beta (PHKB), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025269796.1

ORF Insert Sequence:

ATGGCCAGCT CACCTGATCC AGTCGTCCCT TCTCCATCAG CTTTCTTAAG GTCTGATGAG 
AAGAACTGGC TCAGAGAGTG CCCAAGTGTA GAAAGCTGTG GACATAGCTG TGACTGTAGC 
CGAGGCTCAG TTTATGAACC TCTGAAAAGC ATTAATCTTC CGAGACCTGA TGAAACTCTG 
TGGGATAAAC TGGATCACTA TTACAGAATT GTCAAGTCAA CATTGCTGCT GTATCAAAGT 
CCAACCACAG GTCTCTTTCC CACTAAAACA TGTGGTAATG ATCAGAAGGC CAAGGTTCAT 
GACAGTCTGT ACTGTGCTGC TGGTGCCTGG GCTTTGGCTC TTGCATACAG GCGCATTGAT 
GATGACAAGG GAAGGACCCA TGAGCTGGAG CACTCGGCTA TAAAATGTAT GAGAGGAATT 
CTCTACTGCT ACATGCGTCA GGCCGATAAG GTCCAGCAAT TTAAGCAGGA TCCACGCCCA 
ACAACATGTC TTCACTCCAT TTTCAATGTA CATACAGGAG ATGAGATCCT CTCCTATGAG 
GAATATGGTC ATCTTCAGAT AAATGCAGTG TCCCTCTATC TCCTTTACCT TGTCGAAATG 
ATTTCCTCAG GACTCCAGAT TATCTACAAC ACTGATGAGG TCTCTTTTAT TCAGAACCTT 
GTATTTTGTG TAGAAAGAGT TTACCGGGTG CCTGACTTCG GTGTCTGGGA AAGAGGAAGC 
AAATATAATA ATGGCAGCAC AGAGCTGCAT TCGAGCTCTG TTGGCTTAGC AAAAGCAGCT 
CTAGAAGCAA TTAATGGATT CAACCTCTTT GGCAACCAGG GCTGTTCTTG GTCAGTTATA 
TTTGTGGATC TCGATGCCCA CAATCGCAAC CGGCAAACTT TGTGCTCGTT GTTACCCAGA 
GAATCAAGAT CTCATAACAC AGATGCTGCC CTGCTCCCCT GCATCAGTTA TCCTGCGTTT 
GCCTTGGATG ATGAAGTTCT CTTTAGCCAG ACGCTTGATA AAGTGATTAG AAAATTAAAA 
GGAAAATATG GATTTAAGCG TTTCTTGAGA GATGGATATA GAACATCATT GGAAGATCCC 
AATAGACGCT ACTACAAACC AGCTGAAATT AAGCTGTTTG ATGGCATTGA ATGTGAATTT 
CCCATATTTT TCCTTTACAT GATGATTGAT GGTGTTTTTA GAGGCAATCC CAAGCAAGTA 
AAAGAGTATC AGGATCTTCT GACTCCAGTA CTTCATCACA CCACAGAAGG TTATCCTGTC 
ATACCAAAGT ACTATTATGT GCCAGCTGAT TTTGTAGAGT ATGAAAAAAG AAACCCTGGT 
AGTCAGAAGC GATTCCCTAG CAACTGTGGC CGTGATGGAA AACTGTTTCT TTGGGGACAA 
GCCCTTTATG TCATTGCAAA ACTCCTGGCT GATGAATTAA TTAGTCCTAA AGACATTGAT 
CCTGTTCAGC GTTTTGTCCC TCTACAGAAT CAACGAAATG TGAGCATGAG GTTTTCCAAT 
CAGGGTCCAC TGGAAAATGA TTTGGTTGTT TATGTAGCAC TCATAGCAGA AAGCCAACGC 
CTTCAAGTTT TCCTCAACAC ATATGGTATT CAGACTCAAA CTCCTCAACA GGTGGAACCC 
ATTCAGATCT GGGCCCAGCA GGAGCTGGTG AAAGCTTATT TCCATCTGGG TATCAATGAA 
AAATTAGGAC TCTGCGGAAG GCCAGATAGA CCTATTGGCT GCCTTGGTAC ATCAAAGATT 
TATCGCATTC TAGGAAAGAC TGTGGTTTGT TACCCTATCA TCTTTGACCT TAGTGATTTC 
TACATGTCCC AAGATGTTTT GCTGCTGATA GATGACATAA AGAATGCACT CCAGTTTATT 
AAGCAATACT GGAAAATGCA TGGACGTCCA CTTTTCCTTG TTCTCATCCG GGAAGACAAT 
ATAAGAGGTA GCCGATTCAA TCCCATATTA GATATGCTGG CAGCATTTAA GAAAGGAATG 
GTTGGAGGAG TCAAAGTTCA TGTTGATCGT CTACAGACAC TAATATCTGG AGCTGTGGTT 
GAACAACTTG ACTTCTTACG AATCAGTGAA ATAGAAGAGC TTCCAGAATT TAAGAGTTTT 
GAGGAATTAG AAGTTCCCAA ACATTCAAAA GTCAAACGAC AAAGCAGCAC CTCCAATGCT 
CCCGAACAGG AACAGAAGCC AGATGTTACC GTGACTGAAT GGAGAAACAA ACCCACCCAT 
GAAATTCTTC AAAAACTGAA TGACTGTAGT TGTCTGGCTA GCCAAGCCAT CCTACTGGGT 
ATACTGCTCA AAAGAGAAGG CCCCAACTTC ATCACAAAGG ACGGTACTGT TTCTGATCAC 
ATTGAGAGAG TCTATAGAAG AGCTGGCAGC AAAAAACTTT GGTCGGTGGT GCGCCATGCA 
GCAAGTCTTT TAAGTAAACT AGTGGACAGC CTGGCCCCAT CCATTACTAA TGTTTTAGTG 
CAGGGCAAAC AGGTAACTCT GGGTGCCTTT GGGCATGAAG AAGAAGTTAT CTCTAATCCT 
TTGTCTCCGG GAGTGATTAA GAACATCATC TATTACAAGT GTAATACCCA TGATGAGAGG 
GAGGCAGTAA TTCAGCAAGA ACTGGTCATC CACATTGGCT GGATCATCTC CAATAACCCA 
GAATTATTCA GTGGCATGCT GAAAATACGA ATTGGGTGGA TCATCCATGC TATGGAGTAT 
GAACTACAGA TCCGTGGTGG AAACAAGCCA GCCAGAGACT TATATCAGCT GTCACCCAGT 
GAAGTAAAAC AGCTTCTATT GGATATTCTC CAGCCTCAAC AGAATGGAAG ATGTTGGCTG 
AACAGGCGGC AGATCGATGG CTCTTTGAAT AGAACCCCCC CTGGGTTCTA TGACAGAGTA 
TGGCAGATCC TGGAACGCAC ACCCAATGGA ATCATTGTAG CCGGGAGGCA TTTGCCCCAG 
CAACCAACCC TGTCAGATAT GACCATGTAT GAGATGAATT TCTCTCTCCT TGTTGAAGAC 
ATGTTGGGAA ATATTGACCA GCCAAAATAC AGACAGATTG TCGTGGAGTT ACTAATGGTC 
GTATCCATTG TACTGGAAAG AAACCCTGAG CTAGAATTTC AAGACAAAGT AGATCTTGAC 
AAACTGGTGA AAGAAGCATT TCATGAATTT CAAAAAGATG AGAGTCGACT AAAGGAAATT 
GAAAAACAAG ATGACATGAC TTCCTTTTAC AACACTCCCC CTCTGGGAAA AAGAGGAACA 
TGCAGCTATT TGACAAAGGT CGTGATGAAT CTGCTGCTGG AAGGAGAAGT CAAGCCAAGC 
AATGATGACC CGTGTCTGGT TAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKB ( XM_025269797.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025125582.1 Bubalus bubalis phosphorylase kinase regulatory subunit beta (PHKB), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu53301
Clone ID Related Accession (Same CDS sequence)	XM_025269796.1 , XM_025269797.1 , XM_025269798.1
Accession Version	XM_025269797.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	phosphorylase b kinase regulatory subunit beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037562.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT CACCTGATCC AGTCGTCCCT TCTCCATCAG CTTTCTTAAG GTCTGATGAG 
AAGAACTGGC TCAGAGAGTG CCCAAGTGTA GAAAGCTGTG GACATAGCTG TGACTGTAGC 
CGAGGCTCAG TTTATGAACC TCTGAAAAGC ATTAATCTTC CGAGACCTGA TGAAACTCTG 
TGGGATAAAC TGGATCACTA TTACAGAATT GTCAAGTCAA CATTGCTGCT GTATCAAAGT 
CCAACCACAG GTCTCTTTCC CACTAAAACA TGTGGTAATG ATCAGAAGGC CAAGGTTCAT 
GACAGTCTGT ACTGTGCTGC TGGTGCCTGG GCTTTGGCTC TTGCATACAG GCGCATTGAT 
GATGACAAGG GAAGGACCCA TGAGCTGGAG CACTCGGCTA TAAAATGTAT GAGAGGAATT 
CTCTACTGCT ACATGCGTCA GGCCGATAAG GTCCAGCAAT TTAAGCAGGA TCCACGCCCA 
ACAACATGTC TTCACTCCAT TTTCAATGTA CATACAGGAG ATGAGATCCT CTCCTATGAG 
GAATATGGTC ATCTTCAGAT AAATGCAGTG TCCCTCTATC TCCTTTACCT TGTCGAAATG 
ATTTCCTCAG GACTCCAGAT TATCTACAAC ACTGATGAGG TCTCTTTTAT TCAGAACCTT 
GTATTTTGTG TAGAAAGAGT TTACCGGGTG CCTGACTTCG GTGTCTGGGA AAGAGGAAGC 
AAATATAATA ATGGCAGCAC AGAGCTGCAT TCGAGCTCTG TTGGCTTAGC AAAAGCAGCT 
CTAGAAGCAA TTAATGGATT CAACCTCTTT GGCAACCAGG GCTGTTCTTG GTCAGTTATA 
TTTGTGGATC TCGATGCCCA CAATCGCAAC CGGCAAACTT TGTGCTCGTT GTTACCCAGA 
GAATCAAGAT CTCATAACAC AGATGCTGCC CTGCTCCCCT GCATCAGTTA TCCTGCGTTT 
GCCTTGGATG ATGAAGTTCT CTTTAGCCAG ACGCTTGATA AAGTGATTAG AAAATTAAAA 
GGAAAATATG GATTTAAGCG TTTCTTGAGA GATGGATATA GAACATCATT GGAAGATCCC 
AATAGACGCT ACTACAAACC AGCTGAAATT AAGCTGTTTG ATGGCATTGA ATGTGAATTT 
CCCATATTTT TCCTTTACAT GATGATTGAT GGTGTTTTTA GAGGCAATCC CAAGCAAGTA 
AAAGAGTATC AGGATCTTCT GACTCCAGTA CTTCATCACA CCACAGAAGG TTATCCTGTC 
ATACCAAAGT ACTATTATGT GCCAGCTGAT TTTGTAGAGT ATGAAAAAAG AAACCCTGGT 
AGTCAGAAGC GATTCCCTAG CAACTGTGGC CGTGATGGAA AACTGTTTCT TTGGGGACAA 
GCCCTTTATG TCATTGCAAA ACTCCTGGCT GATGAATTAA TTAGTCCTAA AGACATTGAT 
CCTGTTCAGC GTTTTGTCCC TCTACAGAAT CAACGAAATG TGAGCATGAG GTTTTCCAAT 
CAGGGTCCAC TGGAAAATGA TTTGGTTGTT TATGTAGCAC TCATAGCAGA AAGCCAACGC 
CTTCAAGTTT TCCTCAACAC ATATGGTATT CAGACTCAAA CTCCTCAACA GGTGGAACCC 
ATTCAGATCT GGGCCCAGCA GGAGCTGGTG AAAGCTTATT TCCATCTGGG TATCAATGAA 
AAATTAGGAC TCTGCGGAAG GCCAGATAGA CCTATTGGCT GCCTTGGTAC ATCAAAGATT 
TATCGCATTC TAGGAAAGAC TGTGGTTTGT TACCCTATCA TCTTTGACCT TAGTGATTTC 
TACATGTCCC AAGATGTTTT GCTGCTGATA GATGACATAA AGAATGCACT CCAGTTTATT 
AAGCAATACT GGAAAATGCA TGGACGTCCA CTTTTCCTTG TTCTCATCCG GGAAGACAAT 
ATAAGAGGTA GCCGATTCAA TCCCATATTA GATATGCTGG CAGCATTTAA GAAAGGAATG 
GTTGGAGGAG TCAAAGTTCA TGTTGATCGT CTACAGACAC TAATATCTGG AGCTGTGGTT 
GAACAACTTG ACTTCTTACG AATCAGTGAA ATAGAAGAGC TTCCAGAATT TAAGAGTTTT 
GAGGAATTAG AAGTTCCCAA ACATTCAAAA GTCAAACGAC AAAGCAGCAC CTCCAATGCT 
CCCGAACAGG AACAGAAGCC AGATGTTACC GTGACTGAAT GGAGAAACAA ACCCACCCAT 
GAAATTCTTC AAAAACTGAA TGACTGTAGT TGTCTGGCTA GCCAAGCCAT CCTACTGGGT 
ATACTGCTCA AAAGAGAAGG CCCCAACTTC ATCACAAAGG ACGGTACTGT TTCTGATCAC 
ATTGAGAGAG TCTATAGAAG AGCTGGCAGC AAAAAACTTT GGTCGGTGGT GCGCCATGCA 
GCAAGTCTTT TAAGTAAACT AGTGGACAGC CTGGCCCCAT CCATTACTAA TGTTTTAGTG 
CAGGGCAAAC AGGTAACTCT GGGTGCCTTT GGGCATGAAG AAGAAGTTAT CTCTAATCCT 
TTGTCTCCGG GAGTGATTAA GAACATCATC TATTACAAGT GTAATACCCA TGATGAGAGG 
GAGGCAGTAA TTCAGCAAGA ACTGGTCATC CACATTGGCT GGATCATCTC CAATAACCCA 
GAATTATTCA GTGGCATGCT GAAAATACGA ATTGGGTGGA TCATCCATGC TATGGAGTAT 
GAACTACAGA TCCGTGGTGG AAACAAGCCA GCCAGAGACT TATATCAGCT GTCACCCAGT 
GAAGTAAAAC AGCTTCTATT GGATATTCTC CAGCCTCAAC AGAATGGAAG ATGTTGGCTG 
AACAGGCGGC AGATCGATGG CTCTTTGAAT AGAACCCCCC CTGGGTTCTA TGACAGAGTA 
TGGCAGATCC TGGAACGCAC ACCCAATGGA ATCATTGTAG CCGGGAGGCA TTTGCCCCAG 
CAACCAACCC TGTCAGATAT GACCATGTAT GAGATGAATT TCTCTCTCCT TGTTGAAGAC 
ATGTTGGGAA ATATTGACCA GCCAAAATAC AGACAGATTG TCGTGGAGTT ACTAATGGTC 
GTATCCATTG TACTGGAAAG AAACCCTGAG CTAGAATTTC AAGACAAAGT AGATCTTGAC 
AAACTGGTGA AAGAAGCATT TCATGAATTT CAAAAAGATG AGAGTCGACT AAAGGAAATT 
GAAAAACAAG ATGACATGAC TTCCTTTTAC AACACTCCCC CTCTGGGAAA AAGAGGAACA 
TGCAGCTATT TGACAAAGGT CGTGATGAAT CTGCTGCTGG AAGGAGAAGT CAAGCCAAGC 
AATGATGACC CGTGTCTGGT TAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025125582.1
CDS	186..3512
Translation	MASSPDPVVPSPSAFLRSDEKNWLRECPSVESCGHSCDCSRGSVYEPLKSINLPRPDETLWDKLDHYYRIVKSTLLLYQSPTTGLFPTKTCGNDQKAKVHDSLYCAAGAWALALAYRRIDDDKGRTHELEHSAIKCMRGILYCYMRQADKVQQFKQDPRPTTCLHSIFNVHTGDEILSYEEYGHLQINAVSLYLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERVYRVPDFGVWERGSKYNNGSTELHSSSVGLAKAALEAINGFNLFGNQGCSWSVIFVDLDAHNRNRQTLCSLLPRESRSHNTDAALLPCISYPAFALDDEVLFSQTLDKVIRKLKGKYGFKRFLRDGYRTSLEDPNRRYYKPAEIKLFDGIECEFPIFFLYMMIDGVFRGNPKQVKEYQDLLTPVLHHTTEGYPVIPKYYYVPADFVEYEKRNPGSQKRFPSNCGRDGKLFLWGQALYVIAKLLADELISPKDIDPVQRFVPLQNQRNVSMRFSNQGPLENDLVVYVALIAESQRLQVFLNTYGIQTQTPQQVEPIQIWAQQELVKAYFHLGINEKLGLCGRPDRPIGCLGTSKIYRILGKTVVCYPIIFDLSDFYMSQDVLLLIDDIKNALQFIKQYWKMHGRPLFLVLIREDNIRGSRFNPILDMLAAFKKGMVGGVKVHVDRLQTLISGAVVEQLDFLRISEIEELPEFKSFEELEVPKHSKVKRQSSTSNAPEQEQKPDVTVTEWRNKPTHEILQKLNDCSCLASQAILLGILLKREGPNFITKDGTVSDHIERVYRRAGSKKLWSVVRHAASLLSKLVDSLAPSITNVLVQGKQVTLGAFGHEEEVISNPLSPGVIKNIIYYKCNTHDEREAVIQQELVIHIGWIISNNPELFSGMLKIRIGWIIHAMEYELQIRGGNKPARDLYQLSPSEVKQLLLDILQPQQNGRCWLNRRQIDGSLNRTPPGFYDRVWQILERTPNGIIVAGRHLPQQPTLSDMTMYEMNFSLLVEDMLGNIDQPKYRQIVVELLMVVSIVLERNPELEFQDKVDLDKLVKEAFHEFQKDESRLKEIEKQDDMTSFYNTPPLGKRGTCSYLTKVVMNLLLEGEVKPSNDDPCLVS

Target ORF information:

RefSeq Version	XM_025269797.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis phosphorylase kinase regulatory subunit beta (PHKB), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025269797.1

ORF Insert Sequence:

ATGGCCAGCT CACCTGATCC AGTCGTCCCT TCTCCATCAG CTTTCTTAAG GTCTGATGAG 
AAGAACTGGC TCAGAGAGTG CCCAAGTGTA GAAAGCTGTG GACATAGCTG TGACTGTAGC 
CGAGGCTCAG TTTATGAACC TCTGAAAAGC ATTAATCTTC CGAGACCTGA TGAAACTCTG 
TGGGATAAAC TGGATCACTA TTACAGAATT GTCAAGTCAA CATTGCTGCT GTATCAAAGT 
CCAACCACAG GTCTCTTTCC CACTAAAACA TGTGGTAATG ATCAGAAGGC CAAGGTTCAT 
GACAGTCTGT ACTGTGCTGC TGGTGCCTGG GCTTTGGCTC TTGCATACAG GCGCATTGAT 
GATGACAAGG GAAGGACCCA TGAGCTGGAG CACTCGGCTA TAAAATGTAT GAGAGGAATT 
CTCTACTGCT ACATGCGTCA GGCCGATAAG GTCCAGCAAT TTAAGCAGGA TCCACGCCCA 
ACAACATGTC TTCACTCCAT TTTCAATGTA CATACAGGAG ATGAGATCCT CTCCTATGAG 
GAATATGGTC ATCTTCAGAT AAATGCAGTG TCCCTCTATC TCCTTTACCT TGTCGAAATG 
ATTTCCTCAG GACTCCAGAT TATCTACAAC ACTGATGAGG TCTCTTTTAT TCAGAACCTT 
GTATTTTGTG TAGAAAGAGT TTACCGGGTG CCTGACTTCG GTGTCTGGGA AAGAGGAAGC 
AAATATAATA ATGGCAGCAC AGAGCTGCAT TCGAGCTCTG TTGGCTTAGC AAAAGCAGCT 
CTAGAAGCAA TTAATGGATT CAACCTCTTT GGCAACCAGG GCTGTTCTTG GTCAGTTATA 
TTTGTGGATC TCGATGCCCA CAATCGCAAC CGGCAAACTT TGTGCTCGTT GTTACCCAGA 
GAATCAAGAT CTCATAACAC AGATGCTGCC CTGCTCCCCT GCATCAGTTA TCCTGCGTTT 
GCCTTGGATG ATGAAGTTCT CTTTAGCCAG ACGCTTGATA AAGTGATTAG AAAATTAAAA 
GGAAAATATG GATTTAAGCG TTTCTTGAGA GATGGATATA GAACATCATT GGAAGATCCC 
AATAGACGCT ACTACAAACC AGCTGAAATT AAGCTGTTTG ATGGCATTGA ATGTGAATTT 
CCCATATTTT TCCTTTACAT GATGATTGAT GGTGTTTTTA GAGGCAATCC CAAGCAAGTA 
AAAGAGTATC AGGATCTTCT GACTCCAGTA CTTCATCACA CCACAGAAGG TTATCCTGTC 
ATACCAAAGT ACTATTATGT GCCAGCTGAT TTTGTAGAGT ATGAAAAAAG AAACCCTGGT 
AGTCAGAAGC GATTCCCTAG CAACTGTGGC CGTGATGGAA AACTGTTTCT TTGGGGACAA 
GCCCTTTATG TCATTGCAAA ACTCCTGGCT GATGAATTAA TTAGTCCTAA AGACATTGAT 
CCTGTTCAGC GTTTTGTCCC TCTACAGAAT CAACGAAATG TGAGCATGAG GTTTTCCAAT 
CAGGGTCCAC TGGAAAATGA TTTGGTTGTT TATGTAGCAC TCATAGCAGA AAGCCAACGC 
CTTCAAGTTT TCCTCAACAC ATATGGTATT CAGACTCAAA CTCCTCAACA GGTGGAACCC 
ATTCAGATCT GGGCCCAGCA GGAGCTGGTG AAAGCTTATT TCCATCTGGG TATCAATGAA 
AAATTAGGAC TCTGCGGAAG GCCAGATAGA CCTATTGGCT GCCTTGGTAC ATCAAAGATT 
TATCGCATTC TAGGAAAGAC TGTGGTTTGT TACCCTATCA TCTTTGACCT TAGTGATTTC 
TACATGTCCC AAGATGTTTT GCTGCTGATA GATGACATAA AGAATGCACT CCAGTTTATT 
AAGCAATACT GGAAAATGCA TGGACGTCCA CTTTTCCTTG TTCTCATCCG GGAAGACAAT 
ATAAGAGGTA GCCGATTCAA TCCCATATTA GATATGCTGG CAGCATTTAA GAAAGGAATG 
GTTGGAGGAG TCAAAGTTCA TGTTGATCGT CTACAGACAC TAATATCTGG AGCTGTGGTT 
GAACAACTTG ACTTCTTACG AATCAGTGAA ATAGAAGAGC TTCCAGAATT TAAGAGTTTT 
GAGGAATTAG AAGTTCCCAA ACATTCAAAA GTCAAACGAC AAAGCAGCAC CTCCAATGCT 
CCCGAACAGG AACAGAAGCC AGATGTTACC GTGACTGAAT GGAGAAACAA ACCCACCCAT 
GAAATTCTTC AAAAACTGAA TGACTGTAGT TGTCTGGCTA GCCAAGCCAT CCTACTGGGT 
ATACTGCTCA AAAGAGAAGG CCCCAACTTC ATCACAAAGG ACGGTACTGT TTCTGATCAC 
ATTGAGAGAG TCTATAGAAG AGCTGGCAGC AAAAAACTTT GGTCGGTGGT GCGCCATGCA 
GCAAGTCTTT TAAGTAAACT AGTGGACAGC CTGGCCCCAT CCATTACTAA TGTTTTAGTG 
CAGGGCAAAC AGGTAACTCT GGGTGCCTTT GGGCATGAAG AAGAAGTTAT CTCTAATCCT 
TTGTCTCCGG GAGTGATTAA GAACATCATC TATTACAAGT GTAATACCCA TGATGAGAGG 
GAGGCAGTAA TTCAGCAAGA ACTGGTCATC CACATTGGCT GGATCATCTC CAATAACCCA 
GAATTATTCA GTGGCATGCT GAAAATACGA ATTGGGTGGA TCATCCATGC TATGGAGTAT 
GAACTACAGA TCCGTGGTGG AAACAAGCCA GCCAGAGACT TATATCAGCT GTCACCCAGT 
GAAGTAAAAC AGCTTCTATT GGATATTCTC CAGCCTCAAC AGAATGGAAG ATGTTGGCTG 
AACAGGCGGC AGATCGATGG CTCTTTGAAT AGAACCCCCC CTGGGTTCTA TGACAGAGTA 
TGGCAGATCC TGGAACGCAC ACCCAATGGA ATCATTGTAG CCGGGAGGCA TTTGCCCCAG 
CAACCAACCC TGTCAGATAT GACCATGTAT GAGATGAATT TCTCTCTCCT TGTTGAAGAC 
ATGTTGGGAA ATATTGACCA GCCAAAATAC AGACAGATTG TCGTGGAGTT ACTAATGGTC 
GTATCCATTG TACTGGAAAG AAACCCTGAG CTAGAATTTC AAGACAAAGT AGATCTTGAC 
AAACTGGTGA AAGAAGCATT TCATGAATTT CAAAAAGATG AGAGTCGACT AAAGGAAATT 
GAAAAACAAG ATGACATGAC TTCCTTTTAC AACACTCCCC CTCTGGGAAA AAGAGGAACA 
TGCAGCTATT TGACAAAGGT CGTGATGAAT CTGCTGCTGG AAGGAGAAGT CAAGCCAAGC 
AATGATGACC CGTGTCTGGT TAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKB ( XM_025269798.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025125583.1 Bubalus bubalis phosphorylase kinase regulatory subunit beta (PHKB), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu53301
Clone ID Related Accession (Same CDS sequence)	XM_025269796.1 , XM_025269797.1 , XM_025269798.1
Accession Version	XM_025269798.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	phosphorylase b kinase regulatory subunit beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037562.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT CACCTGATCC AGTCGTCCCT TCTCCATCAG CTTTCTTAAG GTCTGATGAG 
AAGAACTGGC TCAGAGAGTG CCCAAGTGTA GAAAGCTGTG GACATAGCTG TGACTGTAGC 
CGAGGCTCAG TTTATGAACC TCTGAAAAGC ATTAATCTTC CGAGACCTGA TGAAACTCTG 
TGGGATAAAC TGGATCACTA TTACAGAATT GTCAAGTCAA CATTGCTGCT GTATCAAAGT 
CCAACCACAG GTCTCTTTCC CACTAAAACA TGTGGTAATG ATCAGAAGGC CAAGGTTCAT 
GACAGTCTGT ACTGTGCTGC TGGTGCCTGG GCTTTGGCTC TTGCATACAG GCGCATTGAT 
GATGACAAGG GAAGGACCCA TGAGCTGGAG CACTCGGCTA TAAAATGTAT GAGAGGAATT 
CTCTACTGCT ACATGCGTCA GGCCGATAAG GTCCAGCAAT TTAAGCAGGA TCCACGCCCA 
ACAACATGTC TTCACTCCAT TTTCAATGTA CATACAGGAG ATGAGATCCT CTCCTATGAG 
GAATATGGTC ATCTTCAGAT AAATGCAGTG TCCCTCTATC TCCTTTACCT TGTCGAAATG 
ATTTCCTCAG GACTCCAGAT TATCTACAAC ACTGATGAGG TCTCTTTTAT TCAGAACCTT 
GTATTTTGTG TAGAAAGAGT TTACCGGGTG CCTGACTTCG GTGTCTGGGA AAGAGGAAGC 
AAATATAATA ATGGCAGCAC AGAGCTGCAT TCGAGCTCTG TTGGCTTAGC AAAAGCAGCT 
CTAGAAGCAA TTAATGGATT CAACCTCTTT GGCAACCAGG GCTGTTCTTG GTCAGTTATA 
TTTGTGGATC TCGATGCCCA CAATCGCAAC CGGCAAACTT TGTGCTCGTT GTTACCCAGA 
GAATCAAGAT CTCATAACAC AGATGCTGCC CTGCTCCCCT GCATCAGTTA TCCTGCGTTT 
GCCTTGGATG ATGAAGTTCT CTTTAGCCAG ACGCTTGATA AAGTGATTAG AAAATTAAAA 
GGAAAATATG GATTTAAGCG TTTCTTGAGA GATGGATATA GAACATCATT GGAAGATCCC 
AATAGACGCT ACTACAAACC AGCTGAAATT AAGCTGTTTG ATGGCATTGA ATGTGAATTT 
CCCATATTTT TCCTTTACAT GATGATTGAT GGTGTTTTTA GAGGCAATCC CAAGCAAGTA 
AAAGAGTATC AGGATCTTCT GACTCCAGTA CTTCATCACA CCACAGAAGG TTATCCTGTC 
ATACCAAAGT ACTATTATGT GCCAGCTGAT TTTGTAGAGT ATGAAAAAAG AAACCCTGGT 
AGTCAGAAGC GATTCCCTAG CAACTGTGGC CGTGATGGAA AACTGTTTCT TTGGGGACAA 
GCCCTTTATG TCATTGCAAA ACTCCTGGCT GATGAATTAA TTAGTCCTAA AGACATTGAT 
CCTGTTCAGC GTTTTGTCCC TCTACAGAAT CAACGAAATG TGAGCATGAG GTTTTCCAAT 
CAGGGTCCAC TGGAAAATGA TTTGGTTGTT TATGTAGCAC TCATAGCAGA AAGCCAACGC 
CTTCAAGTTT TCCTCAACAC ATATGGTATT CAGACTCAAA CTCCTCAACA GGTGGAACCC 
ATTCAGATCT GGGCCCAGCA GGAGCTGGTG AAAGCTTATT TCCATCTGGG TATCAATGAA 
AAATTAGGAC TCTGCGGAAG GCCAGATAGA CCTATTGGCT GCCTTGGTAC ATCAAAGATT 
TATCGCATTC TAGGAAAGAC TGTGGTTTGT TACCCTATCA TCTTTGACCT TAGTGATTTC 
TACATGTCCC AAGATGTTTT GCTGCTGATA GATGACATAA AGAATGCACT CCAGTTTATT 
AAGCAATACT GGAAAATGCA TGGACGTCCA CTTTTCCTTG TTCTCATCCG GGAAGACAAT 
ATAAGAGGTA GCCGATTCAA TCCCATATTA GATATGCTGG CAGCATTTAA GAAAGGAATG 
GTTGGAGGAG TCAAAGTTCA TGTTGATCGT CTACAGACAC TAATATCTGG AGCTGTGGTT 
GAACAACTTG ACTTCTTACG AATCAGTGAA ATAGAAGAGC TTCCAGAATT TAAGAGTTTT 
GAGGAATTAG AAGTTCCCAA ACATTCAAAA GTCAAACGAC AAAGCAGCAC CTCCAATGCT 
CCCGAACAGG AACAGAAGCC AGATGTTACC GTGACTGAAT GGAGAAACAA ACCCACCCAT 
GAAATTCTTC AAAAACTGAA TGACTGTAGT TGTCTGGCTA GCCAAGCCAT CCTACTGGGT 
ATACTGCTCA AAAGAGAAGG CCCCAACTTC ATCACAAAGG ACGGTACTGT TTCTGATCAC 
ATTGAGAGAG TCTATAGAAG AGCTGGCAGC AAAAAACTTT GGTCGGTGGT GCGCCATGCA 
GCAAGTCTTT TAAGTAAACT AGTGGACAGC CTGGCCCCAT CCATTACTAA TGTTTTAGTG 
CAGGGCAAAC AGGTAACTCT GGGTGCCTTT GGGCATGAAG AAGAAGTTAT CTCTAATCCT 
TTGTCTCCGG GAGTGATTAA GAACATCATC TATTACAAGT GTAATACCCA TGATGAGAGG 
GAGGCAGTAA TTCAGCAAGA ACTGGTCATC CACATTGGCT GGATCATCTC CAATAACCCA 
GAATTATTCA GTGGCATGCT GAAAATACGA ATTGGGTGGA TCATCCATGC TATGGAGTAT 
GAACTACAGA TCCGTGGTGG AAACAAGCCA GCCAGAGACT TATATCAGCT GTCACCCAGT 
GAAGTAAAAC AGCTTCTATT GGATATTCTC CAGCCTCAAC AGAATGGAAG ATGTTGGCTG 
AACAGGCGGC AGATCGATGG CTCTTTGAAT AGAACCCCCC CTGGGTTCTA TGACAGAGTA 
TGGCAGATCC TGGAACGCAC ACCCAATGGA ATCATTGTAG CCGGGAGGCA TTTGCCCCAG 
CAACCAACCC TGTCAGATAT GACCATGTAT GAGATGAATT TCTCTCTCCT TGTTGAAGAC 
ATGTTGGGAA ATATTGACCA GCCAAAATAC AGACAGATTG TCGTGGAGTT ACTAATGGTC 
GTATCCATTG TACTGGAAAG AAACCCTGAG CTAGAATTTC AAGACAAAGT AGATCTTGAC 
AAACTGGTGA AAGAAGCATT TCATGAATTT CAAAAAGATG AGAGTCGACT AAAGGAAATT 
GAAAAACAAG ATGACATGAC TTCCTTTTAC AACACTCCCC CTCTGGGAAA AAGAGGAACA 
TGCAGCTATT TGACAAAGGT CGTGATGAAT CTGCTGCTGG AAGGAGAAGT CAAGCCAAGC 
AATGATGACC CGTGTCTGGT TAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025125583.1
CDS	264..3590
Translation	MASSPDPVVPSPSAFLRSDEKNWLRECPSVESCGHSCDCSRGSVYEPLKSINLPRPDETLWDKLDHYYRIVKSTLLLYQSPTTGLFPTKTCGNDQKAKVHDSLYCAAGAWALALAYRRIDDDKGRTHELEHSAIKCMRGILYCYMRQADKVQQFKQDPRPTTCLHSIFNVHTGDEILSYEEYGHLQINAVSLYLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERVYRVPDFGVWERGSKYNNGSTELHSSSVGLAKAALEAINGFNLFGNQGCSWSVIFVDLDAHNRNRQTLCSLLPRESRSHNTDAALLPCISYPAFALDDEVLFSQTLDKVIRKLKGKYGFKRFLRDGYRTSLEDPNRRYYKPAEIKLFDGIECEFPIFFLYMMIDGVFRGNPKQVKEYQDLLTPVLHHTTEGYPVIPKYYYVPADFVEYEKRNPGSQKRFPSNCGRDGKLFLWGQALYVIAKLLADELISPKDIDPVQRFVPLQNQRNVSMRFSNQGPLENDLVVYVALIAESQRLQVFLNTYGIQTQTPQQVEPIQIWAQQELVKAYFHLGINEKLGLCGRPDRPIGCLGTSKIYRILGKTVVCYPIIFDLSDFYMSQDVLLLIDDIKNALQFIKQYWKMHGRPLFLVLIREDNIRGSRFNPILDMLAAFKKGMVGGVKVHVDRLQTLISGAVVEQLDFLRISEIEELPEFKSFEELEVPKHSKVKRQSSTSNAPEQEQKPDVTVTEWRNKPTHEILQKLNDCSCLASQAILLGILLKREGPNFITKDGTVSDHIERVYRRAGSKKLWSVVRHAASLLSKLVDSLAPSITNVLVQGKQVTLGAFGHEEEVISNPLSPGVIKNIIYYKCNTHDEREAVIQQELVIHIGWIISNNPELFSGMLKIRIGWIIHAMEYELQIRGGNKPARDLYQLSPSEVKQLLLDILQPQQNGRCWLNRRQIDGSLNRTPPGFYDRVWQILERTPNGIIVAGRHLPQQPTLSDMTMYEMNFSLLVEDMLGNIDQPKYRQIVVELLMVVSIVLERNPELEFQDKVDLDKLVKEAFHEFQKDESRLKEIEKQDDMTSFYNTPPLGKRGTCSYLTKVVMNLLLEGEVKPSNDDPCLVS

Target ORF information:

RefSeq Version	XM_025269798.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis phosphorylase kinase regulatory subunit beta (PHKB), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025269798.1

ORF Insert Sequence:

ATGGCCAGCT CACCTGATCC AGTCGTCCCT TCTCCATCAG CTTTCTTAAG GTCTGATGAG 
AAGAACTGGC TCAGAGAGTG CCCAAGTGTA GAAAGCTGTG GACATAGCTG TGACTGTAGC 
CGAGGCTCAG TTTATGAACC TCTGAAAAGC ATTAATCTTC CGAGACCTGA TGAAACTCTG 
TGGGATAAAC TGGATCACTA TTACAGAATT GTCAAGTCAA CATTGCTGCT GTATCAAAGT 
CCAACCACAG GTCTCTTTCC CACTAAAACA TGTGGTAATG ATCAGAAGGC CAAGGTTCAT 
GACAGTCTGT ACTGTGCTGC TGGTGCCTGG GCTTTGGCTC TTGCATACAG GCGCATTGAT 
GATGACAAGG GAAGGACCCA TGAGCTGGAG CACTCGGCTA TAAAATGTAT GAGAGGAATT 
CTCTACTGCT ACATGCGTCA GGCCGATAAG GTCCAGCAAT TTAAGCAGGA TCCACGCCCA 
ACAACATGTC TTCACTCCAT TTTCAATGTA CATACAGGAG ATGAGATCCT CTCCTATGAG 
GAATATGGTC ATCTTCAGAT AAATGCAGTG TCCCTCTATC TCCTTTACCT TGTCGAAATG 
ATTTCCTCAG GACTCCAGAT TATCTACAAC ACTGATGAGG TCTCTTTTAT TCAGAACCTT 
GTATTTTGTG TAGAAAGAGT TTACCGGGTG CCTGACTTCG GTGTCTGGGA AAGAGGAAGC 
AAATATAATA ATGGCAGCAC AGAGCTGCAT TCGAGCTCTG TTGGCTTAGC AAAAGCAGCT 
CTAGAAGCAA TTAATGGATT CAACCTCTTT GGCAACCAGG GCTGTTCTTG GTCAGTTATA 
TTTGTGGATC TCGATGCCCA CAATCGCAAC CGGCAAACTT TGTGCTCGTT GTTACCCAGA 
GAATCAAGAT CTCATAACAC AGATGCTGCC CTGCTCCCCT GCATCAGTTA TCCTGCGTTT 
GCCTTGGATG ATGAAGTTCT CTTTAGCCAG ACGCTTGATA AAGTGATTAG AAAATTAAAA 
GGAAAATATG GATTTAAGCG TTTCTTGAGA GATGGATATA GAACATCATT GGAAGATCCC 
AATAGACGCT ACTACAAACC AGCTGAAATT AAGCTGTTTG ATGGCATTGA ATGTGAATTT 
CCCATATTTT TCCTTTACAT GATGATTGAT GGTGTTTTTA GAGGCAATCC CAAGCAAGTA 
AAAGAGTATC AGGATCTTCT GACTCCAGTA CTTCATCACA CCACAGAAGG TTATCCTGTC 
ATACCAAAGT ACTATTATGT GCCAGCTGAT TTTGTAGAGT ATGAAAAAAG AAACCCTGGT 
AGTCAGAAGC GATTCCCTAG CAACTGTGGC CGTGATGGAA AACTGTTTCT TTGGGGACAA 
GCCCTTTATG TCATTGCAAA ACTCCTGGCT GATGAATTAA TTAGTCCTAA AGACATTGAT 
CCTGTTCAGC GTTTTGTCCC TCTACAGAAT CAACGAAATG TGAGCATGAG GTTTTCCAAT 
CAGGGTCCAC TGGAAAATGA TTTGGTTGTT TATGTAGCAC TCATAGCAGA AAGCCAACGC 
CTTCAAGTTT TCCTCAACAC ATATGGTATT CAGACTCAAA CTCCTCAACA GGTGGAACCC 
ATTCAGATCT GGGCCCAGCA GGAGCTGGTG AAAGCTTATT TCCATCTGGG TATCAATGAA 
AAATTAGGAC TCTGCGGAAG GCCAGATAGA CCTATTGGCT GCCTTGGTAC ATCAAAGATT 
TATCGCATTC TAGGAAAGAC TGTGGTTTGT TACCCTATCA TCTTTGACCT TAGTGATTTC 
TACATGTCCC AAGATGTTTT GCTGCTGATA GATGACATAA AGAATGCACT CCAGTTTATT 
AAGCAATACT GGAAAATGCA TGGACGTCCA CTTTTCCTTG TTCTCATCCG GGAAGACAAT 
ATAAGAGGTA GCCGATTCAA TCCCATATTA GATATGCTGG CAGCATTTAA GAAAGGAATG 
GTTGGAGGAG TCAAAGTTCA TGTTGATCGT CTACAGACAC TAATATCTGG AGCTGTGGTT 
GAACAACTTG ACTTCTTACG AATCAGTGAA ATAGAAGAGC TTCCAGAATT TAAGAGTTTT 
GAGGAATTAG AAGTTCCCAA ACATTCAAAA GTCAAACGAC AAAGCAGCAC CTCCAATGCT 
CCCGAACAGG AACAGAAGCC AGATGTTACC GTGACTGAAT GGAGAAACAA ACCCACCCAT 
GAAATTCTTC AAAAACTGAA TGACTGTAGT TGTCTGGCTA GCCAAGCCAT CCTACTGGGT 
ATACTGCTCA AAAGAGAAGG CCCCAACTTC ATCACAAAGG ACGGTACTGT TTCTGATCAC 
ATTGAGAGAG TCTATAGAAG AGCTGGCAGC AAAAAACTTT GGTCGGTGGT GCGCCATGCA 
GCAAGTCTTT TAAGTAAACT AGTGGACAGC CTGGCCCCAT CCATTACTAA TGTTTTAGTG 
CAGGGCAAAC AGGTAACTCT GGGTGCCTTT GGGCATGAAG AAGAAGTTAT CTCTAATCCT 
TTGTCTCCGG GAGTGATTAA GAACATCATC TATTACAAGT GTAATACCCA TGATGAGAGG 
GAGGCAGTAA TTCAGCAAGA ACTGGTCATC CACATTGGCT GGATCATCTC CAATAACCCA 
GAATTATTCA GTGGCATGCT GAAAATACGA ATTGGGTGGA TCATCCATGC TATGGAGTAT 
GAACTACAGA TCCGTGGTGG AAACAAGCCA GCCAGAGACT TATATCAGCT GTCACCCAGT 
GAAGTAAAAC AGCTTCTATT GGATATTCTC CAGCCTCAAC AGAATGGAAG ATGTTGGCTG 
AACAGGCGGC AGATCGATGG CTCTTTGAAT AGAACCCCCC CTGGGTTCTA TGACAGAGTA 
TGGCAGATCC TGGAACGCAC ACCCAATGGA ATCATTGTAG CCGGGAGGCA TTTGCCCCAG 
CAACCAACCC TGTCAGATAT GACCATGTAT GAGATGAATT TCTCTCTCCT TGTTGAAGAC 
ATGTTGGGAA ATATTGACCA GCCAAAATAC AGACAGATTG TCGTGGAGTT ACTAATGGTC 
GTATCCATTG TACTGGAAAG AAACCCTGAG CTAGAATTTC AAGACAAAGT AGATCTTGAC 
AAACTGGTGA AAGAAGCATT TCATGAATTT CAAAAAGATG AGAGTCGACT AAAGGAAATT 
GAAAAACAAG ATGACATGAC TTCCTTTTAC AACACTCCCC CTCTGGGAAA AAGAGGAACA 
TGCAGCTATT TGACAAAGGT CGTGATGAAT CTGCTGCTGG AAGGAGAAGT CAAGCCAAGC 
AATGATGACC CGTGTCTGGT TAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKB ( XM_025269799.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025125584.1 Bubalus bubalis phosphorylase kinase regulatory subunit beta (PHKB), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu53302
Clone ID Related Accession (Same CDS sequence)	XM_025269799.1
Accession Version	XM_025269799.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	phosphorylase b kinase regulatory subunit beta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037562.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT CACCTGATCC AGTCGTCCCT TCTCCATCAG CTTTCTTAAG GTCTGATGAG 
AAGAACTGGC TCAGAGAGTG CCCAAGTGTA GAAAGCTGTG GACATAGCTG TGACTGTAGC 
CGAGGCTCAG TTTATGAACC TCTGAAAAGC ATTAATCTTC CGAGACCTGA TGAAACTCTG 
TGGGATAAAC TGGATCACTA TTACAGAATT GTCAAGTCAA CATTGCTGCT GTATCAAAGT 
CCAACCACAG GTCTCTTTCC CACTAAAACA TGTGGTAATG ATCAGAAGGC CAAGGTTCAT 
GACAGTCTGT ACTGTGCTGC TGGTGCCTGG GCTTTGGCTC TTGCATACAG GCGCATTGAT 
GATGACAAGG GAAGGACCCA TGAGCTGGAG CACTCGGCTA TAAAATGTAT GAGAGGAATT 
CTCTACTGCT ACATGCGTCA GGCCGATAAG GTCCAGCAAT TTAAGCAGGA TCCACGCCCA 
ACAACATGTC TTCACTCCAT TTTCAATGTA CATACAGGAG ATGAGATCCT CTCCTATGAG 
GAATATGGTC ATCTTCAGAT AAATGCAGTG TCCCTCTATC TCCTTTACCT TGTCGAAATG 
ATTTCCTCAG GACTCCAGAT TATCTACAAC ACTGATGAGG TCTCTTTTAT TCAGAACCTT 
GTATTTTGTG TAGAAAGAGT TTACCGGGTG CCTGACTTCG GTGTCTGGGA AAGAGGAAGC 
AAATATAATA ATGGCAGCAC AGAGCTGCAT TCGAGCTCTG TTGGCTTAGC AAAAGCAGCT 
CTAGAAGCAA TTAATGGATT CAACCTCTTT GGCAACCAGG GCTGTTCTTG GTCAGTTATA 
TTTGTGGATC TCGATGCCCA CAATCGCAAC CGGCAAACTT TGTGCTCGTT GTTACCCAGA 
GAATCAAGAT CTCATAACAC AGATGCTGCC CTGCTCCCCT GCATCAGTTA TCCTGCGTTT 
GCCTTGGATG ATGAAGTTCT CTTTAGCCAG ACGCTTGATA AAGTGATTAG AAAATTAAAA 
GGAAAATATG GATTTAAGCG TTTCTTGAGA GATGGATATA GAACATCATT GGAAGATCCC 
AATAGACGCT ACTACAAACC AGCTGAAATT AAGCTGTTTG ATGGCATTGA ATGTGAATTT 
CCCATATTTT TCCTTTACAT GATGATTGAT GGTGTTTTTA GAGGCAATCC CAAGCAAGTA 
AAAGAGTATC AGGATCTTCT GACTCCAGTA CTTCATCACA CCACAGAAGG TTATCCTGTC 
ATACCAAAGT ACTATTATGT GCCAGCTGAT TTTGTAGAGT ATGAAAAAAG AAACCCTGGT 
AGTCAGAAGC GATTCCCTAG CAACTGTGGC CGTGATGGAA AACTGTTTCT TTGGGGACAA 
GCCCTTTATG TCATTGCAAA ACTCCTGGCT GATGAATTAA TTAGTCCTAA AGACATTGAT 
CCTGTTCAGC GTTTTGTCCC TCTACAGAAT CAACGAAATG TGAGCATGAG GTTTTCCAAT 
CAGGGTCCAC TGGAAAATGA TTTGGTTGTT TATGTAGCAC TCATAGCAGA AAGCCAACGC 
CTTCAAGTTT TCCTCAACAC ATATGGTATT CAGACTCAAA CTCCTCAACA GGTGGAACCC 
ATTCAGATCT GGGCCCAGCA GGAGCTGGTG AAAGCTTATT TCCATCTGGG TATCAATGAA 
AAATTAGGAC TCTGCGGAAG GCCAGATAGA CCTATTGGCT GCCTTGGTAC ATCAAAGATT 
TATCGCATTC TAGGAAAGAC TGTGGTTTGT TACCCTATCA TCTTTGACCT TAGTGATTTC 
TACATGTCCC AAGATGTTTT GCTGCTGATA GATGACATAA AGAATGCACT CCAGTTTATT 
AAGCAATACT GGAAAATGCA TGGACGTCCA CTTTTCCTTG TTCTCATCCG GGAAGACAAT 
ATAAGAGGTA GCCGATTCAA TCCCATATTA GATATGCTGG CAGCATTTAA GAAAGGAATG 
GTTGGAGGAG TCAAAGTTCA TGTTGATCGT CTACAGACAC TAATATCTGG AGCTGTGGTT 
GAACAACTTG ACTTCTTACG AATCAGTGAA ATAGAAGAGC TTCCAGAATT TAAGAGTTTT 
GAGGAATTAG AAGTTCCCAA ACATTCAAAA GTCAAACGAC AAAGCAGCAC CTCCAATGCT 
CCCGAACAGG AACAGAAGCC AGATGTTACC GTGACTGAAT GGAGAAACAA ACCCACCCAT 
GAAATTCTTC AAAAACTGAA TGACTGTAGT TGTCTGGCTA GCCAAGCCAT CCTACTGGGT 
ATACTGCTCA AAAGAGAAGG CCCCAACTTC ATCACAAAGG ACGGTACTGT TTCTGATCAC 
ATTGAGAGAG TCTATAGAAG AGCTGGCAGC AAAAAACTTT GGTTGGCGGT ACGCTACGGG 
GCTGCATTTA CGCAGAAATT TTCTTCCTCT ATAGCCCCAC ACATTACTAC TTTTCTGGTA 
CATGGGAAAC AGGTAACTCT GGGTGCCTTT GGGCATGAAG AAGAAGTTAT CTCTAATCCT 
TTGTCTCCGG GAGTGATTAA GAACATCATC TATTACAAGT GTAATACCCA TGATGAGAGG 
GAGGCAGTAA TTCAGCAAGA ACTGGTCATC CACATTGGCT GGATCATCTC CAATAACCCA 
GAATTATTCA GTGGCATGCT GAAAATACGA ATTGGGTGGA TCATCCATGC TATGGAGTAT 
GAACTACAGA TCCGTGGTGG AAACAAGCCA GCCAGAGACT TATATCAGCT GTCACCCAGT 
GAAGTAAAAC AGCTTCTATT GGATATTCTC CAGCCTCAAC AGAATGGAAG ATGTTGGCTG 
AACAGGCGGC AGATCGATGG CTCTTTGAAT AGAACCCCCC CTGGGTTCTA TGACAGAGTA 
TGGCAGATCC TGGAACGCAC ACCCAATGGA ATCATTGTAG CCGGGAGGCA TTTGCCCCAG 
CAACCAACCC TGTCAGATAT GACCATGTAT GAGATGAATT TCTCTCTCCT TGTTGAAGAC 
ATGTTGGGAA ATATTGACCA GCCAAAATAC AGACAGATTG TCGTGGAGTT ACTAATGGTC 
GTATCCATTG TACTGGAAAG AAACCCTGAG CTAGAATTTC AAGACAAAGT AGATCTTGAC 
AAACTGGTGA AAGAAGCATT TCATGAATTT CAAAAAGATG AGAGTCGACT AAAGGAAATT 
GAAAAACAAG ATGACATGAC TTCCTTTTAC AACACTCCCC CTCTGGGAAA AAGAGGAACA 
TGCAGCTATT TGACAAAGGT CGTGATGAAT CTGCTGCTGG AAGGAGAAGT CAAGCCAAGC 
AATGATGACC CGTGTCTGGT TAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025125584.1
CDS	186..3512
Translation	MASSPDPVVPSPSAFLRSDEKNWLRECPSVESCGHSCDCSRGSVYEPLKSINLPRPDETLWDKLDHYYRIVKSTLLLYQSPTTGLFPTKTCGNDQKAKVHDSLYCAAGAWALALAYRRIDDDKGRTHELEHSAIKCMRGILYCYMRQADKVQQFKQDPRPTTCLHSIFNVHTGDEILSYEEYGHLQINAVSLYLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERVYRVPDFGVWERGSKYNNGSTELHSSSVGLAKAALEAINGFNLFGNQGCSWSVIFVDLDAHNRNRQTLCSLLPRESRSHNTDAALLPCISYPAFALDDEVLFSQTLDKVIRKLKGKYGFKRFLRDGYRTSLEDPNRRYYKPAEIKLFDGIECEFPIFFLYMMIDGVFRGNPKQVKEYQDLLTPVLHHTTEGYPVIPKYYYVPADFVEYEKRNPGSQKRFPSNCGRDGKLFLWGQALYVIAKLLADELISPKDIDPVQRFVPLQNQRNVSMRFSNQGPLENDLVVYVALIAESQRLQVFLNTYGIQTQTPQQVEPIQIWAQQELVKAYFHLGINEKLGLCGRPDRPIGCLGTSKIYRILGKTVVCYPIIFDLSDFYMSQDVLLLIDDIKNALQFIKQYWKMHGRPLFLVLIREDNIRGSRFNPILDMLAAFKKGMVGGVKVHVDRLQTLISGAVVEQLDFLRISEIEELPEFKSFEELEVPKHSKVKRQSSTSNAPEQEQKPDVTVTEWRNKPTHEILQKLNDCSCLASQAILLGILLKREGPNFITKDGTVSDHIERVYRRAGSKKLWLAVRYGAAFTQKFSSSIAPHITTFLVHGKQVTLGAFGHEEEVISNPLSPGVIKNIIYYKCNTHDEREAVIQQELVIHIGWIISNNPELFSGMLKIRIGWIIHAMEYELQIRGGNKPARDLYQLSPSEVKQLLLDILQPQQNGRCWLNRRQIDGSLNRTPPGFYDRVWQILERTPNGIIVAGRHLPQQPTLSDMTMYEMNFSLLVEDMLGNIDQPKYRQIVVELLMVVSIVLERNPELEFQDKVDLDKLVKEAFHEFQKDESRLKEIEKQDDMTSFYNTPPLGKRGTCSYLTKVVMNLLLEGEVKPSNDDPCLVS

Target ORF information:

RefSeq Version	XM_025269799.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis phosphorylase kinase regulatory subunit beta (PHKB), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025269799.1

ORF Insert Sequence:

ATGGCCAGCT CACCTGATCC AGTCGTCCCT TCTCCATCAG CTTTCTTAAG GTCTGATGAG 
AAGAACTGGC TCAGAGAGTG CCCAAGTGTA GAAAGCTGTG GACATAGCTG TGACTGTAGC 
CGAGGCTCAG TTTATGAACC TCTGAAAAGC ATTAATCTTC CGAGACCTGA TGAAACTCTG 
TGGGATAAAC TGGATCACTA TTACAGAATT GTCAAGTCAA CATTGCTGCT GTATCAAAGT 
CCAACCACAG GTCTCTTTCC CACTAAAACA TGTGGTAATG ATCAGAAGGC CAAGGTTCAT 
GACAGTCTGT ACTGTGCTGC TGGTGCCTGG GCTTTGGCTC TTGCATACAG GCGCATTGAT 
GATGACAAGG GAAGGACCCA TGAGCTGGAG CACTCGGCTA TAAAATGTAT GAGAGGAATT 
CTCTACTGCT ACATGCGTCA GGCCGATAAG GTCCAGCAAT TTAAGCAGGA TCCACGCCCA 
ACAACATGTC TTCACTCCAT TTTCAATGTA CATACAGGAG ATGAGATCCT CTCCTATGAG 
GAATATGGTC ATCTTCAGAT AAATGCAGTG TCCCTCTATC TCCTTTACCT TGTCGAAATG 
ATTTCCTCAG GACTCCAGAT TATCTACAAC ACTGATGAGG TCTCTTTTAT TCAGAACCTT 
GTATTTTGTG TAGAAAGAGT TTACCGGGTG CCTGACTTCG GTGTCTGGGA AAGAGGAAGC 
AAATATAATA ATGGCAGCAC AGAGCTGCAT TCGAGCTCTG TTGGCTTAGC AAAAGCAGCT 
CTAGAAGCAA TTAATGGATT CAACCTCTTT GGCAACCAGG GCTGTTCTTG GTCAGTTATA 
TTTGTGGATC TCGATGCCCA CAATCGCAAC CGGCAAACTT TGTGCTCGTT GTTACCCAGA 
GAATCAAGAT CTCATAACAC AGATGCTGCC CTGCTCCCCT GCATCAGTTA TCCTGCGTTT 
GCCTTGGATG ATGAAGTTCT CTTTAGCCAG ACGCTTGATA AAGTGATTAG AAAATTAAAA 
GGAAAATATG GATTTAAGCG TTTCTTGAGA GATGGATATA GAACATCATT GGAAGATCCC 
AATAGACGCT ACTACAAACC AGCTGAAATT AAGCTGTTTG ATGGCATTGA ATGTGAATTT 
CCCATATTTT TCCTTTACAT GATGATTGAT GGTGTTTTTA GAGGCAATCC CAAGCAAGTA 
AAAGAGTATC AGGATCTTCT GACTCCAGTA CTTCATCACA CCACAGAAGG TTATCCTGTC 
ATACCAAAGT ACTATTATGT GCCAGCTGAT TTTGTAGAGT ATGAAAAAAG AAACCCTGGT 
AGTCAGAAGC GATTCCCTAG CAACTGTGGC CGTGATGGAA AACTGTTTCT TTGGGGACAA 
GCCCTTTATG TCATTGCAAA ACTCCTGGCT GATGAATTAA TTAGTCCTAA AGACATTGAT 
CCTGTTCAGC GTTTTGTCCC TCTACAGAAT CAACGAAATG TGAGCATGAG GTTTTCCAAT 
CAGGGTCCAC TGGAAAATGA TTTGGTTGTT TATGTAGCAC TCATAGCAGA AAGCCAACGC 
CTTCAAGTTT TCCTCAACAC ATATGGTATT CAGACTCAAA CTCCTCAACA GGTGGAACCC 
ATTCAGATCT GGGCCCAGCA GGAGCTGGTG AAAGCTTATT TCCATCTGGG TATCAATGAA 
AAATTAGGAC TCTGCGGAAG GCCAGATAGA CCTATTGGCT GCCTTGGTAC ATCAAAGATT 
TATCGCATTC TAGGAAAGAC TGTGGTTTGT TACCCTATCA TCTTTGACCT TAGTGATTTC 
TACATGTCCC AAGATGTTTT GCTGCTGATA GATGACATAA AGAATGCACT CCAGTTTATT 
AAGCAATACT GGAAAATGCA TGGACGTCCA CTTTTCCTTG TTCTCATCCG GGAAGACAAT 
ATAAGAGGTA GCCGATTCAA TCCCATATTA GATATGCTGG CAGCATTTAA GAAAGGAATG 
GTTGGAGGAG TCAAAGTTCA TGTTGATCGT CTACAGACAC TAATATCTGG AGCTGTGGTT 
GAACAACTTG ACTTCTTACG AATCAGTGAA ATAGAAGAGC TTCCAGAATT TAAGAGTTTT 
GAGGAATTAG AAGTTCCCAA ACATTCAAAA GTCAAACGAC AAAGCAGCAC CTCCAATGCT 
CCCGAACAGG AACAGAAGCC AGATGTTACC GTGACTGAAT GGAGAAACAA ACCCACCCAT 
GAAATTCTTC AAAAACTGAA TGACTGTAGT TGTCTGGCTA GCCAAGCCAT CCTACTGGGT 
ATACTGCTCA AAAGAGAAGG CCCCAACTTC ATCACAAAGG ACGGTACTGT TTCTGATCAC 
ATTGAGAGAG TCTATAGAAG AGCTGGCAGC AAAAAACTTT GGTTGGCGGT ACGCTACGGG 
GCTGCATTTA CGCAGAAATT TTCTTCCTCT ATAGCCCCAC ACATTACTAC TTTTCTGGTA 
CATGGGAAAC AGGTAACTCT GGGTGCCTTT GGGCATGAAG AAGAAGTTAT CTCTAATCCT 
TTGTCTCCGG GAGTGATTAA GAACATCATC TATTACAAGT GTAATACCCA TGATGAGAGG 
GAGGCAGTAA TTCAGCAAGA ACTGGTCATC CACATTGGCT GGATCATCTC CAATAACCCA 
GAATTATTCA GTGGCATGCT GAAAATACGA ATTGGGTGGA TCATCCATGC TATGGAGTAT 
GAACTACAGA TCCGTGGTGG AAACAAGCCA GCCAGAGACT TATATCAGCT GTCACCCAGT 
GAAGTAAAAC AGCTTCTATT GGATATTCTC CAGCCTCAAC AGAATGGAAG ATGTTGGCTG 
AACAGGCGGC AGATCGATGG CTCTTTGAAT AGAACCCCCC CTGGGTTCTA TGACAGAGTA 
TGGCAGATCC TGGAACGCAC ACCCAATGGA ATCATTGTAG CCGGGAGGCA TTTGCCCCAG 
CAACCAACCC TGTCAGATAT GACCATGTAT GAGATGAATT TCTCTCTCCT TGTTGAAGAC 
ATGTTGGGAA ATATTGACCA GCCAAAATAC AGACAGATTG TCGTGGAGTT ACTAATGGTC 
GTATCCATTG TACTGGAAAG AAACCCTGAG CTAGAATTTC AAGACAAAGT AGATCTTGAC 
AAACTGGTGA AAGAAGCATT TCATGAATTT CAAAAAGATG AGAGTCGACT AAAGGAAATT 
GAAAAACAAG ATGACATGAC TTCCTTTTAC AACACTCCCC CTCTGGGAAA AAGAGGAACA 
TGCAGCTATT TGACAAAGGT CGTGATGAAT CTGCTGCTGG AAGGAGAAGT CAAGCCAAGC 
AATGATGACC CGTGTCTGGT TAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKB ( XM_025269800.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025125585.1 Bubalus bubalis phosphorylase kinase regulatory subunit beta (PHKB), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBu53303
Clone ID Related Accession (Same CDS sequence)	XM_025269800.1
Accession Version	XM_025269800.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	phosphorylase b kinase regulatory subunit beta isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037562.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGC TCATGGCCGA AGTAAGCTGG AAGGTCTTGG AGCGAAGAGC CCGGACCAAG 
CGCTCAGGCT CAGTTTATGA ACCTCTGAAA AGCATTAATC TTCCGAGACC TGATGAAACT 
CTGTGGGATA AACTGGATCA CTATTACAGA ATTGTCAAGT CAACATTGCT GCTGTATCAA 
AGTCCAACCA CAGGTCTCTT TCCCACTAAA ACATGTGGTA ATGATCAGAA GGCCAAGGTT 
CATGACAGTC TGTACTGTGC TGCTGGTGCC TGGGCTTTGG CTCTTGCATA CAGGCGCATT 
GATGATGACA AGGGAAGGAC CCATGAGCTG GAGCACTCGG CTATAAAATG TATGAGAGGA 
ATTCTCTACT GCTACATGCG TCAGGCCGAT AAGGTCCAGC AATTTAAGCA GGATCCACGC 
CCAACAACAT GTCTTCACTC CATTTTCAAT GTACATACAG GAGATGAGAT CCTCTCCTAT 
GAGGAATATG GTCATCTTCA GATAAATGCA GTGTCCCTCT ATCTCCTTTA CCTTGTCGAA 
ATGATTTCCT CAGGACTCCA GATTATCTAC AACACTGATG AGGTCTCTTT TATTCAGAAC 
CTTGTATTTT GTGTAGAAAG AGTTTACCGG GTGCCTGACT TCGGTGTCTG GGAAAGAGGA 
AGCAAATATA ATAATGGCAG CACAGAGCTG CATTCGAGCT CTGTTGGCTT AGCAAAAGCA 
GCTCTAGAAG CAATTAATGG ATTCAACCTC TTTGGCAACC AGGGCTGTTC TTGGTCAGTT 
ATATTTGTGG ATCTCGATGC CCACAATCGC AACCGGCAAA CTTTGTGCTC GTTGTTACCC 
AGAGAATCAA GATCTCATAA CACAGATGCT GCCCTGCTCC CCTGCATCAG TTATCCTGCG 
TTTGCCTTGG ATGATGAAGT TCTCTTTAGC CAGACGCTTG ATAAAGTGAT TAGAAAATTA 
AAAGGAAAAT ATGGATTTAA GCGTTTCTTG AGAGATGGAT ATAGAACATC ATTGGAAGAT 
CCCAATAGAC GCTACTACAA ACCAGCTGAA ATTAAGCTGT TTGATGGCAT TGAATGTGAA 
TTTCCCATAT TTTTCCTTTA CATGATGATT GATGGTGTTT TTAGAGGCAA TCCCAAGCAA 
GTAAAAGAGT ATCAGGATCT TCTGACTCCA GTACTTCATC ACACCACAGA AGGTTATCCT 
GTCATACCAA AGTACTATTA TGTGCCAGCT GATTTTGTAG AGTATGAAAA AAGAAACCCT 
GGTAGTCAGA AGCGATTCCC TAGCAACTGT GGCCGTGATG GAAAACTGTT TCTTTGGGGA 
CAAGCCCTTT ATGTCATTGC AAAACTCCTG GCTGATGAAT TAATTAGTCC TAAAGACATT 
GATCCTGTTC AGCGTTTTGT CCCTCTACAG AATCAACGAA ATGTGAGCAT GAGGTTTTCC 
AATCAGGGTC CACTGGAAAA TGATTTGGTT GTTTATGTAG CACTCATAGC AGAAAGCCAA 
CGCCTTCAAG TTTTCCTCAA CACATATGGT ATTCAGACTC AAACTCCTCA ACAGGTGGAA 
CCCATTCAGA TCTGGGCCCA GCAGGAGCTG GTGAAAGCTT ATTTCCATCT GGGTATCAAT 
GAAAAATTAG GACTCTGCGG AAGGCCAGAT AGACCTATTG GCTGCCTTGG TACATCAAAG 
ATTTATCGCA TTCTAGGAAA GACTGTGGTT TGTTACCCTA TCATCTTTGA CCTTAGTGAT 
TTCTACATGT CCCAAGATGT TTTGCTGCTG ATAGATGACA TAAAGAATGC ACTCCAGTTT 
ATTAAGCAAT ACTGGAAAAT GCATGGACGT CCACTTTTCC TTGTTCTCAT CCGGGAAGAC 
AATATAAGAG GTAGCCGATT CAATCCCATA TTAGATATGC TGGCAGCATT TAAGAAAGGA 
ATGGTTGGAG GAGTCAAAGT TCATGTTGAT CGTCTACAGA CACTAATATC TGGAGCTGTG 
GTTGAACAAC TTGACTTCTT ACGAATCAGT GAAATAGAAG AGCTTCCAGA ATTTAAGAGT 
TTTGAGGAAT TAGAAGTTCC CAAACATTCA AAAGTCAAAC GACAAAGCAG CACCTCCAAT 
GCTCCCGAAC AGGAACAGAA GCCAGATGTT ACCGTGACTG AATGGAGAAA CAAACCCACC 
CATGAAATTC TTCAAAAACT GAATGACTGT AGTTGTCTGG CTAGCCAAGC CATCCTACTG 
GGTATACTGC TCAAAAGAGA AGGCCCCAAC TTCATCACAA AGGACGGTAC TGTTTCTGAT 
CACATTGAGA GAGTCTATAG AAGAGCTGGC AGCAAAAAAC TTTGGTCGGT GGTGCGCCAT 
GCAGCAAGTC TTTTAAGTAA ACTAGTGGAC AGCCTGGCCC CATCCATTAC TAATGTTTTA 
GTGCAGGGCA AACAGGTAAC TCTGGGTGCC TTTGGGCATG AAGAAGAAGT TATCTCTAAT 
CCTTTGTCTC CGGGAGTGAT TAAGAACATC ATCTATTACA AGTGTAATAC CCATGATGAG 
AGGGAGGCAG TAATTCAGCA AGAACTGGTC ATCCACATTG GCTGGATCAT CTCCAATAAC 
CCAGAATTAT TCAGTGGCAT GCTGAAAATA CGAATTGGGT GGATCATCCA TGCTATGGAG 
TATGAACTAC AGATCCGTGG TGGAAACAAG CCAGCCAGAG ACTTATATCA GCTGTCACCC 
AGTGAAGTAA AACAGCTTCT ATTGGATATT CTCCAGCCTC AACAGAATGG AAGATGTTGG 
CTGAACAGGC GGCAGATCGA TGGCTCTTTG AATAGAACCC CCCCTGGGTT CTATGACAGA 
GTATGGCAGA TCCTGGAACG CACACCCAAT GGAATCATTG TAGCCGGGAG GCATTTGCCC 
CAGCAACCAA CCCTGTCAGA TATGACCATG TATGAGATGA ATTTCTCTCT CCTTGTTGAA 
GACATGTTGG GAAATATTGA CCAGCCAAAA TACAGACAGA TTGTCGTGGA GTTACTAATG 
GTCGTATCCA TTGTACTGGA AAGAAACCCT GAGCTAGAAT TTCAAGACAA AGTAGATCTT 
GACAAACTGG TGAAAGAAGC ATTTCATGAA TTTCAAAAAG ATGAGAGTCG ACTAAAGGAA 
ATTGAAAAAC AAGATGACAT GACTTCCTTT TACAACACTC CCCCTCTGGG AAAAAGAGGA 
ACATGCAGCT ATTTGACAAA GGTCGTGATG AATCTGCTGC TGGAAGGAGA AGTCAAGCCA 
AGCAATGATG ACCCGTGTCT GGTTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025125585.1
CDS	71..3340
Translation	MAGLMAEVSWKVLERRARTKRSGSVYEPLKSINLPRPDETLWDKLDHYYRIVKSTLLLYQSPTTGLFPTKTCGNDQKAKVHDSLYCAAGAWALALAYRRIDDDKGRTHELEHSAIKCMRGILYCYMRQADKVQQFKQDPRPTTCLHSIFNVHTGDEILSYEEYGHLQINAVSLYLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERVYRVPDFGVWERGSKYNNGSTELHSSSVGLAKAALEAINGFNLFGNQGCSWSVIFVDLDAHNRNRQTLCSLLPRESRSHNTDAALLPCISYPAFALDDEVLFSQTLDKVIRKLKGKYGFKRFLRDGYRTSLEDPNRRYYKPAEIKLFDGIECEFPIFFLYMMIDGVFRGNPKQVKEYQDLLTPVLHHTTEGYPVIPKYYYVPADFVEYEKRNPGSQKRFPSNCGRDGKLFLWGQALYVIAKLLADELISPKDIDPVQRFVPLQNQRNVSMRFSNQGPLENDLVVYVALIAESQRLQVFLNTYGIQTQTPQQVEPIQIWAQQELVKAYFHLGINEKLGLCGRPDRPIGCLGTSKIYRILGKTVVCYPIIFDLSDFYMSQDVLLLIDDIKNALQFIKQYWKMHGRPLFLVLIREDNIRGSRFNPILDMLAAFKKGMVGGVKVHVDRLQTLISGAVVEQLDFLRISEIEELPEFKSFEELEVPKHSKVKRQSSTSNAPEQEQKPDVTVTEWRNKPTHEILQKLNDCSCLASQAILLGILLKREGPNFITKDGTVSDHIERVYRRAGSKKLWSVVRHAASLLSKLVDSLAPSITNVLVQGKQVTLGAFGHEEEVISNPLSPGVIKNIIYYKCNTHDEREAVIQQELVIHIGWIISNNPELFSGMLKIRIGWIIHAMEYELQIRGGNKPARDLYQLSPSEVKQLLLDILQPQQNGRCWLNRRQIDGSLNRTPPGFYDRVWQILERTPNGIIVAGRHLPQQPTLSDMTMYEMNFSLLVEDMLGNIDQPKYRQIVVELLMVVSIVLERNPELEFQDKVDLDKLVKEAFHEFQKDESRLKEIEKQDDMTSFYNTPPLGKRGTCSYLTKVVMNLLLEGEVKPSNDDPCLVS

Target ORF information:

RefSeq Version	XM_025269800.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis phosphorylase kinase regulatory subunit beta (PHKB), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025269800.1

ORF Insert Sequence:

ATGGCGGGGC TCATGGCCGA AGTAAGCTGG AAGGTCTTGG AGCGAAGAGC CCGGACCAAG 
CGCTCAGGCT CAGTTTATGA ACCTCTGAAA AGCATTAATC TTCCGAGACC TGATGAAACT 
CTGTGGGATA AACTGGATCA CTATTACAGA ATTGTCAAGT CAACATTGCT GCTGTATCAA 
AGTCCAACCA CAGGTCTCTT TCCCACTAAA ACATGTGGTA ATGATCAGAA GGCCAAGGTT 
CATGACAGTC TGTACTGTGC TGCTGGTGCC TGGGCTTTGG CTCTTGCATA CAGGCGCATT 
GATGATGACA AGGGAAGGAC CCATGAGCTG GAGCACTCGG CTATAAAATG TATGAGAGGA 
ATTCTCTACT GCTACATGCG TCAGGCCGAT AAGGTCCAGC AATTTAAGCA GGATCCACGC 
CCAACAACAT GTCTTCACTC CATTTTCAAT GTACATACAG GAGATGAGAT CCTCTCCTAT 
GAGGAATATG GTCATCTTCA GATAAATGCA GTGTCCCTCT ATCTCCTTTA CCTTGTCGAA 
ATGATTTCCT CAGGACTCCA GATTATCTAC AACACTGATG AGGTCTCTTT TATTCAGAAC 
CTTGTATTTT GTGTAGAAAG AGTTTACCGG GTGCCTGACT TCGGTGTCTG GGAAAGAGGA 
AGCAAATATA ATAATGGCAG CACAGAGCTG CATTCGAGCT CTGTTGGCTT AGCAAAAGCA 
GCTCTAGAAG CAATTAATGG ATTCAACCTC TTTGGCAACC AGGGCTGTTC TTGGTCAGTT 
ATATTTGTGG ATCTCGATGC CCACAATCGC AACCGGCAAA CTTTGTGCTC GTTGTTACCC 
AGAGAATCAA GATCTCATAA CACAGATGCT GCCCTGCTCC CCTGCATCAG TTATCCTGCG 
TTTGCCTTGG ATGATGAAGT TCTCTTTAGC CAGACGCTTG ATAAAGTGAT TAGAAAATTA 
AAAGGAAAAT ATGGATTTAA GCGTTTCTTG AGAGATGGAT ATAGAACATC ATTGGAAGAT 
CCCAATAGAC GCTACTACAA ACCAGCTGAA ATTAAGCTGT TTGATGGCAT TGAATGTGAA 
TTTCCCATAT TTTTCCTTTA CATGATGATT GATGGTGTTT TTAGAGGCAA TCCCAAGCAA 
GTAAAAGAGT ATCAGGATCT TCTGACTCCA GTACTTCATC ACACCACAGA AGGTTATCCT 
GTCATACCAA AGTACTATTA TGTGCCAGCT GATTTTGTAG AGTATGAAAA AAGAAACCCT 
GGTAGTCAGA AGCGATTCCC TAGCAACTGT GGCCGTGATG GAAAACTGTT TCTTTGGGGA 
CAAGCCCTTT ATGTCATTGC AAAACTCCTG GCTGATGAAT TAATTAGTCC TAAAGACATT 
GATCCTGTTC AGCGTTTTGT CCCTCTACAG AATCAACGAA ATGTGAGCAT GAGGTTTTCC 
AATCAGGGTC CACTGGAAAA TGATTTGGTT GTTTATGTAG CACTCATAGC AGAAAGCCAA 
CGCCTTCAAG TTTTCCTCAA CACATATGGT ATTCAGACTC AAACTCCTCA ACAGGTGGAA 
CCCATTCAGA TCTGGGCCCA GCAGGAGCTG GTGAAAGCTT ATTTCCATCT GGGTATCAAT 
GAAAAATTAG GACTCTGCGG AAGGCCAGAT AGACCTATTG GCTGCCTTGG TACATCAAAG 
ATTTATCGCA TTCTAGGAAA GACTGTGGTT TGTTACCCTA TCATCTTTGA CCTTAGTGAT 
TTCTACATGT CCCAAGATGT TTTGCTGCTG ATAGATGACA TAAAGAATGC ACTCCAGTTT 
ATTAAGCAAT ACTGGAAAAT GCATGGACGT CCACTTTTCC TTGTTCTCAT CCGGGAAGAC 
AATATAAGAG GTAGCCGATT CAATCCCATA TTAGATATGC TGGCAGCATT TAAGAAAGGA 
ATGGTTGGAG GAGTCAAAGT TCATGTTGAT CGTCTACAGA CACTAATATC TGGAGCTGTG 
GTTGAACAAC TTGACTTCTT ACGAATCAGT GAAATAGAAG AGCTTCCAGA ATTTAAGAGT 
TTTGAGGAAT TAGAAGTTCC CAAACATTCA AAAGTCAAAC GACAAAGCAG CACCTCCAAT 
GCTCCCGAAC AGGAACAGAA GCCAGATGTT ACCGTGACTG AATGGAGAAA CAAACCCACC 
CATGAAATTC TTCAAAAACT GAATGACTGT AGTTGTCTGG CTAGCCAAGC CATCCTACTG 
GGTATACTGC TCAAAAGAGA AGGCCCCAAC TTCATCACAA AGGACGGTAC TGTTTCTGAT 
CACATTGAGA GAGTCTATAG AAGAGCTGGC AGCAAAAAAC TTTGGTCGGT GGTGCGCCAT 
GCAGCAAGTC TTTTAAGTAA ACTAGTGGAC AGCCTGGCCC CATCCATTAC TAATGTTTTA 
GTGCAGGGCA AACAGGTAAC TCTGGGTGCC TTTGGGCATG AAGAAGAAGT TATCTCTAAT 
CCTTTGTCTC CGGGAGTGAT TAAGAACATC ATCTATTACA AGTGTAATAC CCATGATGAG 
AGGGAGGCAG TAATTCAGCA AGAACTGGTC ATCCACATTG GCTGGATCAT CTCCAATAAC 
CCAGAATTAT TCAGTGGCAT GCTGAAAATA CGAATTGGGT GGATCATCCA TGCTATGGAG 
TATGAACTAC AGATCCGTGG TGGAAACAAG CCAGCCAGAG ACTTATATCA GCTGTCACCC 
AGTGAAGTAA AACAGCTTCT ATTGGATATT CTCCAGCCTC AACAGAATGG AAGATGTTGG 
CTGAACAGGC GGCAGATCGA TGGCTCTTTG AATAGAACCC CCCCTGGGTT CTATGACAGA 
GTATGGCAGA TCCTGGAACG CACACCCAAT GGAATCATTG TAGCCGGGAG GCATTTGCCC 
CAGCAACCAA CCCTGTCAGA TATGACCATG TATGAGATGA ATTTCTCTCT CCTTGTTGAA 
GACATGTTGG GAAATATTGA CCAGCCAAAA TACAGACAGA TTGTCGTGGA GTTACTAATG 
GTCGTATCCA TTGTACTGGA AAGAAACCCT GAGCTAGAAT TTCAAGACAA AGTAGATCTT 
GACAAACTGG TGAAAGAAGC ATTTCATGAA TTTCAAAAAG ATGAGAGTCG ACTAAAGGAA 
ATTGAAAAAC AAGATGACAT GACTTCCTTT TACAACACTC CCCCTCTGGG AAAAAGAGGA 
ACATGCAGCT ATTTGACAAA GGTCGTGATG AATCTGCTGC TGGAAGGAGA AGTCAAGCCA 
AGCAATGATG ACCCGTGTCT GGTTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKB ( XM_025269801.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025125586.1 Bubalus bubalis phosphorylase kinase regulatory subunit beta (PHKB), transcript variant X6, mRNA.

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CloneID	OBu53304
Clone ID Related Accession (Same CDS sequence)	XM_025269801.1
Accession Version	XM_025269801.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	phosphorylase b kinase regulatory subunit beta isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037562.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGC TCATGGCCGA AGTAAGCTGG AAGGTCTTGG AGCGAAGAGC CCGGACCAAG 
CGCTCAGGCT CAGTTTATGA ACCTCTGAAA AGCATTAATC TTCCGAGACC TGATGAAACT 
CTGTGGGATA AACTGGATCA CTATTACAGA ATTGTCAAGT CAACATTGCT GCTGTATCAA 
AGTCCAACCA CAGGTCTCTT TCCCACTAAA ACATGTGGTA ATGATCAGAA GGCCAAGGTT 
CATGACAGTC TGTACTGTGC TGCTGGTGCC TGGGCTTTGG CTCTTGCATA CAGGCGCATT 
GATGATGACA AGGGAAGGAC CCATGAGCTG GAGCACTCGG CTATAAAATG TATGAGAGGA 
ATTCTCTACT GCTACATGCG TCAGGCCGAT AAGGTCCAGC AATTTAAGCA GGATCCACGC 
CCAACAACAT GTCTTCACTC CATTTTCAAT GTACATACAG GAGATGAGAT CCTCTCCTAT 
GAGGAATATG GTCATCTTCA GATAAATGCA GTGTCCCTCT ATCTCCTTTA CCTTGTCGAA 
ATGATTTCCT CAGGACTCCA GATTATCTAC AACACTGATG AGGTCTCTTT TATTCAGAAC 
CTTGTATTTT GTGTAGAAAG AGTTTACCGG GTGCCTGACT TCGGTGTCTG GGAAAGAGGA 
AGCAAATATA ATAATGGCAG CACAGAGCTG CATTCGAGCT CTGTTGGCTT AGCAAAAGCA 
GCTCTAGAAG CAATTAATGG ATTCAACCTC TTTGGCAACC AGGGCTGTTC TTGGTCAGTT 
ATATTTGTGG ATCTCGATGC CCACAATCGC AACCGGCAAA CTTTGTGCTC GTTGTTACCC 
AGAGAATCAA GATCTCATAA CACAGATGCT GCCCTGCTCC CCTGCATCAG TTATCCTGCG 
TTTGCCTTGG ATGATGAAGT TCTCTTTAGC CAGACGCTTG ATAAAGTGAT TAGAAAATTA 
AAAGGAAAAT ATGGATTTAA GCGTTTCTTG AGAGATGGAT ATAGAACATC ATTGGAAGAT 
CCCAATAGAC GCTACTACAA ACCAGCTGAA ATTAAGCTGT TTGATGGCAT TGAATGTGAA 
TTTCCCATAT TTTTCCTTTA CATGATGATT GATGGTGTTT TTAGAGGCAA TCCCAAGCAA 
GTAAAAGAGT ATCAGGATCT TCTGACTCCA GTACTTCATC ACACCACAGA AGGTTATCCT 
GTCATACCAA AGTACTATTA TGTGCCAGCT GATTTTGTAG AGTATGAAAA AAGAAACCCT 
GGTAGTCAGA AGCGATTCCC TAGCAACTGT GGCCGTGATG GAAAACTGTT TCTTTGGGGA 
CAAGCCCTTT ATGTCATTGC AAAACTCCTG GCTGATGAAT TAATTAGTCC TAAAGACATT 
GATCCTGTTC AGCGTTTTGT CCCTCTACAG AATCAACGAA ATGTGAGCAT GAGGTTTTCC 
AATCAGGGTC CACTGGAAAA TGATTTGGTT GTTTATGTAG CACTCATAGC AGAAAGCCAA 
CGCCTTCAAG TTTTCCTCAA CACATATGGT ATTCAGACTC AAACTCCTCA ACAGGTGGAA 
CCCATTCAGA TCTGGGCCCA GCAGGAGCTG GTGAAAGCTT ATTTCCATCT GGGTATCAAT 
GAAAAATTAG GACTCTGCGG AAGGCCAGAT AGACCTATTG GCTGCCTTGG TACATCAAAG 
ATTTATCGCA TTCTAGGAAA GACTGTGGTT TGTTACCCTA TCATCTTTGA CCTTAGTGAT 
TTCTACATGT CCCAAGATGT TTTGCTGCTG ATAGATGACA TAAAGAATGC ACTCCAGTTT 
ATTAAGCAAT ACTGGAAAAT GCATGGACGT CCACTTTTCC TTGTTCTCAT CCGGGAAGAC 
AATATAAGAG GTAGCCGATT CAATCCCATA TTAGATATGC TGGCAGCATT TAAGAAAGGA 
ATGGTTGGAG GAGTCAAAGT TCATGTTGAT CGTCTACAGA CACTAATATC TGGAGCTGTG 
GTTGAACAAC TTGACTTCTT ACGAATCAGT GAAATAGAAG AGCTTCCAGA ATTTAAGAGT 
TTTGAGGAAT TAGAAGTTCC CAAACATTCA AAAGTCAAAC GACAAAGCAG CACCTCCAAT 
GCTCCCGAAC AGGAACAGAA GCCAGATGTT ACCGTGACTG AATGGAGAAA CAAACCCACC 
CATGAAATTC TTCAAAAACT GAATGACTGT AGTTGTCTGG CTAGCCAAGC CATCCTACTG 
GGTATACTGC TCAAAAGAGA AGGCCCCAAC TTCATCACAA AGGACGGTAC TGTTTCTGAT 
CACATTGAGA GAGTCTATAG AAGAGCTGGC AGCAAAAAAC TTTGGTTGGC GGTACGCTAC 
GGGGCTGCAT TTACGCAGAA ATTTTCTTCC TCTATAGCCC CACACATTAC TACTTTTCTG 
GTACATGGGA AACAGGTAAC TCTGGGTGCC TTTGGGCATG AAGAAGAAGT TATCTCTAAT 
CCTTTGTCTC CGGGAGTGAT TAAGAACATC ATCTATTACA AGTGTAATAC CCATGATGAG 
AGGGAGGCAG TAATTCAGCA AGAACTGGTC ATCCACATTG GCTGGATCAT CTCCAATAAC 
CCAGAATTAT TCAGTGGCAT GCTGAAAATA CGAATTGGGT GGATCATCCA TGCTATGGAG 
TATGAACTAC AGATCCGTGG TGGAAACAAG CCAGCCAGAG ACTTATATCA GCTGTCACCC 
AGTGAAGTAA AACAGCTTCT ATTGGATATT CTCCAGCCTC AACAGAATGG AAGATGTTGG 
CTGAACAGGC GGCAGATCGA TGGCTCTTTG AATAGAACCC CCCCTGGGTT CTATGACAGA 
GTATGGCAGA TCCTGGAACG CACACCCAAT GGAATCATTG TAGCCGGGAG GCATTTGCCC 
CAGCAACCAA CCCTGTCAGA TATGACCATG TATGAGATGA ATTTCTCTCT CCTTGTTGAA 
GACATGTTGG GAAATATTGA CCAGCCAAAA TACAGACAGA TTGTCGTGGA GTTACTAATG 
GTCGTATCCA TTGTACTGGA AAGAAACCCT GAGCTAGAAT TTCAAGACAA AGTAGATCTT 
GACAAACTGG TGAAAGAAGC ATTTCATGAA TTTCAAAAAG ATGAGAGTCG ACTAAAGGAA 
ATTGAAAAAC AAGATGACAT GACTTCCTTT TACAACACTC CCCCTCTGGG AAAAAGAGGA 
ACATGCAGCT ATTTGACAAA GGTCGTGATG AATCTGCTGC TGGAAGGAGA AGTCAAGCCA 
AGCAATGATG ACCCGTGTCT GGTTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025125586.1
CDS	71..3340
Translation	MAGLMAEVSWKVLERRARTKRSGSVYEPLKSINLPRPDETLWDKLDHYYRIVKSTLLLYQSPTTGLFPTKTCGNDQKAKVHDSLYCAAGAWALALAYRRIDDDKGRTHELEHSAIKCMRGILYCYMRQADKVQQFKQDPRPTTCLHSIFNVHTGDEILSYEEYGHLQINAVSLYLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERVYRVPDFGVWERGSKYNNGSTELHSSSVGLAKAALEAINGFNLFGNQGCSWSVIFVDLDAHNRNRQTLCSLLPRESRSHNTDAALLPCISYPAFALDDEVLFSQTLDKVIRKLKGKYGFKRFLRDGYRTSLEDPNRRYYKPAEIKLFDGIECEFPIFFLYMMIDGVFRGNPKQVKEYQDLLTPVLHHTTEGYPVIPKYYYVPADFVEYEKRNPGSQKRFPSNCGRDGKLFLWGQALYVIAKLLADELISPKDIDPVQRFVPLQNQRNVSMRFSNQGPLENDLVVYVALIAESQRLQVFLNTYGIQTQTPQQVEPIQIWAQQELVKAYFHLGINEKLGLCGRPDRPIGCLGTSKIYRILGKTVVCYPIIFDLSDFYMSQDVLLLIDDIKNALQFIKQYWKMHGRPLFLVLIREDNIRGSRFNPILDMLAAFKKGMVGGVKVHVDRLQTLISGAVVEQLDFLRISEIEELPEFKSFEELEVPKHSKVKRQSSTSNAPEQEQKPDVTVTEWRNKPTHEILQKLNDCSCLASQAILLGILLKREGPNFITKDGTVSDHIERVYRRAGSKKLWLAVRYGAAFTQKFSSSIAPHITTFLVHGKQVTLGAFGHEEEVISNPLSPGVIKNIIYYKCNTHDEREAVIQQELVIHIGWIISNNPELFSGMLKIRIGWIIHAMEYELQIRGGNKPARDLYQLSPSEVKQLLLDILQPQQNGRCWLNRRQIDGSLNRTPPGFYDRVWQILERTPNGIIVAGRHLPQQPTLSDMTMYEMNFSLLVEDMLGNIDQPKYRQIVVELLMVVSIVLERNPELEFQDKVDLDKLVKEAFHEFQKDESRLKEIEKQDDMTSFYNTPPLGKRGTCSYLTKVVMNLLLEGEVKPSNDDPCLVS

Target ORF information:

RefSeq Version	XM_025269801.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis phosphorylase kinase regulatory subunit beta (PHKB), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025269801.1

ORF Insert Sequence:

ATGGCGGGGC TCATGGCCGA AGTAAGCTGG AAGGTCTTGG AGCGAAGAGC CCGGACCAAG 
CGCTCAGGCT CAGTTTATGA ACCTCTGAAA AGCATTAATC TTCCGAGACC TGATGAAACT 
CTGTGGGATA AACTGGATCA CTATTACAGA ATTGTCAAGT CAACATTGCT GCTGTATCAA 
AGTCCAACCA CAGGTCTCTT TCCCACTAAA ACATGTGGTA ATGATCAGAA GGCCAAGGTT 
CATGACAGTC TGTACTGTGC TGCTGGTGCC TGGGCTTTGG CTCTTGCATA CAGGCGCATT 
GATGATGACA AGGGAAGGAC CCATGAGCTG GAGCACTCGG CTATAAAATG TATGAGAGGA 
ATTCTCTACT GCTACATGCG TCAGGCCGAT AAGGTCCAGC AATTTAAGCA GGATCCACGC 
CCAACAACAT GTCTTCACTC CATTTTCAAT GTACATACAG GAGATGAGAT CCTCTCCTAT 
GAGGAATATG GTCATCTTCA GATAAATGCA GTGTCCCTCT ATCTCCTTTA CCTTGTCGAA 
ATGATTTCCT CAGGACTCCA GATTATCTAC AACACTGATG AGGTCTCTTT TATTCAGAAC 
CTTGTATTTT GTGTAGAAAG AGTTTACCGG GTGCCTGACT TCGGTGTCTG GGAAAGAGGA 
AGCAAATATA ATAATGGCAG CACAGAGCTG CATTCGAGCT CTGTTGGCTT AGCAAAAGCA 
GCTCTAGAAG CAATTAATGG ATTCAACCTC TTTGGCAACC AGGGCTGTTC TTGGTCAGTT 
ATATTTGTGG ATCTCGATGC CCACAATCGC AACCGGCAAA CTTTGTGCTC GTTGTTACCC 
AGAGAATCAA GATCTCATAA CACAGATGCT GCCCTGCTCC CCTGCATCAG TTATCCTGCG 
TTTGCCTTGG ATGATGAAGT TCTCTTTAGC CAGACGCTTG ATAAAGTGAT TAGAAAATTA 
AAAGGAAAAT ATGGATTTAA GCGTTTCTTG AGAGATGGAT ATAGAACATC ATTGGAAGAT 
CCCAATAGAC GCTACTACAA ACCAGCTGAA ATTAAGCTGT TTGATGGCAT TGAATGTGAA 
TTTCCCATAT TTTTCCTTTA CATGATGATT GATGGTGTTT TTAGAGGCAA TCCCAAGCAA 
GTAAAAGAGT ATCAGGATCT TCTGACTCCA GTACTTCATC ACACCACAGA AGGTTATCCT 
GTCATACCAA AGTACTATTA TGTGCCAGCT GATTTTGTAG AGTATGAAAA AAGAAACCCT 
GGTAGTCAGA AGCGATTCCC TAGCAACTGT GGCCGTGATG GAAAACTGTT TCTTTGGGGA 
CAAGCCCTTT ATGTCATTGC AAAACTCCTG GCTGATGAAT TAATTAGTCC TAAAGACATT 
GATCCTGTTC AGCGTTTTGT CCCTCTACAG AATCAACGAA ATGTGAGCAT GAGGTTTTCC 
AATCAGGGTC CACTGGAAAA TGATTTGGTT GTTTATGTAG CACTCATAGC AGAAAGCCAA 
CGCCTTCAAG TTTTCCTCAA CACATATGGT ATTCAGACTC AAACTCCTCA ACAGGTGGAA 
CCCATTCAGA TCTGGGCCCA GCAGGAGCTG GTGAAAGCTT ATTTCCATCT GGGTATCAAT 
GAAAAATTAG GACTCTGCGG AAGGCCAGAT AGACCTATTG GCTGCCTTGG TACATCAAAG 
ATTTATCGCA TTCTAGGAAA GACTGTGGTT TGTTACCCTA TCATCTTTGA CCTTAGTGAT 
TTCTACATGT CCCAAGATGT TTTGCTGCTG ATAGATGACA TAAAGAATGC ACTCCAGTTT 
ATTAAGCAAT ACTGGAAAAT GCATGGACGT CCACTTTTCC TTGTTCTCAT CCGGGAAGAC 
AATATAAGAG GTAGCCGATT CAATCCCATA TTAGATATGC TGGCAGCATT TAAGAAAGGA 
ATGGTTGGAG GAGTCAAAGT TCATGTTGAT CGTCTACAGA CACTAATATC TGGAGCTGTG 
GTTGAACAAC TTGACTTCTT ACGAATCAGT GAAATAGAAG AGCTTCCAGA ATTTAAGAGT 
TTTGAGGAAT TAGAAGTTCC CAAACATTCA AAAGTCAAAC GACAAAGCAG CACCTCCAAT 
GCTCCCGAAC AGGAACAGAA GCCAGATGTT ACCGTGACTG AATGGAGAAA CAAACCCACC 
CATGAAATTC TTCAAAAACT GAATGACTGT AGTTGTCTGG CTAGCCAAGC CATCCTACTG 
GGTATACTGC TCAAAAGAGA AGGCCCCAAC TTCATCACAA AGGACGGTAC TGTTTCTGAT 
CACATTGAGA GAGTCTATAG AAGAGCTGGC AGCAAAAAAC TTTGGTTGGC GGTACGCTAC 
GGGGCTGCAT TTACGCAGAA ATTTTCTTCC TCTATAGCCC CACACATTAC TACTTTTCTG 
GTACATGGGA AACAGGTAAC TCTGGGTGCC TTTGGGCATG AAGAAGAAGT TATCTCTAAT 
CCTTTGTCTC CGGGAGTGAT TAAGAACATC ATCTATTACA AGTGTAATAC CCATGATGAG 
AGGGAGGCAG TAATTCAGCA AGAACTGGTC ATCCACATTG GCTGGATCAT CTCCAATAAC 
CCAGAATTAT TCAGTGGCAT GCTGAAAATA CGAATTGGGT GGATCATCCA TGCTATGGAG 
TATGAACTAC AGATCCGTGG TGGAAACAAG CCAGCCAGAG ACTTATATCA GCTGTCACCC 
AGTGAAGTAA AACAGCTTCT ATTGGATATT CTCCAGCCTC AACAGAATGG AAGATGTTGG 
CTGAACAGGC GGCAGATCGA TGGCTCTTTG AATAGAACCC CCCCTGGGTT CTATGACAGA 
GTATGGCAGA TCCTGGAACG CACACCCAAT GGAATCATTG TAGCCGGGAG GCATTTGCCC 
CAGCAACCAA CCCTGTCAGA TATGACCATG TATGAGATGA ATTTCTCTCT CCTTGTTGAA 
GACATGTTGG GAAATATTGA CCAGCCAAAA TACAGACAGA TTGTCGTGGA GTTACTAATG 
GTCGTATCCA TTGTACTGGA AAGAAACCCT GAGCTAGAAT TTCAAGACAA AGTAGATCTT 
GACAAACTGG TGAAAGAAGC ATTTCATGAA TTTCAAAAAG ATGAGAGTCG ACTAAAGGAA 
ATTGAAAAAC AAGATGACAT GACTTCCTTT TACAACACTC CCCCTCTGGG AAAAAGAGGA 
ACATGCAGCT ATTTGACAAA GGTCGTGATG AATCTGCTGC TGGAAGGAGA AGTCAAGCCA 
AGCAATGATG ACCCGTGTCT GGTTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKB ( XM_025269802.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025125587.1 Bubalus bubalis phosphorylase kinase regulatory subunit beta (PHKB), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBu53305
Clone ID Related Accession (Same CDS sequence)	XM_025269802.1
Accession Version	XM_025269802.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	phosphorylase b kinase regulatory subunit beta isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037562.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT CACCTGATCC AGTCGTCCCT TCTCCATCAG CTTTCTTAAG GTCTGGCTCA 
GTTTATGAAC CTCTGAAAAG CATTAATCTT CCGAGACCTG ATGAAACTCT GTGGGATAAA 
CTGGATCACT ATTACAGAAT TGTCAAGTCA ACATTGCTGC TGTATCAAAG TCCAACCACA 
GGTCTCTTTC CCACTAAAAC ATGTGGTAAT GATCAGAAGG CCAAGGTTCA TGACAGTCTG 
TACTGTGCTG CTGGTGCCTG GGCTTTGGCT CTTGCATACA GGCGCATTGA TGATGACAAG 
GGAAGGACCC ATGAGCTGGA GCACTCGGCT ATAAAATGTA TGAGAGGAAT TCTCTACTGC 
TACATGCGTC AGGCCGATAA GGTCCAGCAA TTTAAGCAGG ATCCACGCCC AACAACATGT 
CTTCACTCCA TTTTCAATGT ACATACAGGA GATGAGATCC TCTCCTATGA GGAATATGGT 
CATCTTCAGA TAAATGCAGT GTCCCTCTAT CTCCTTTACC TTGTCGAAAT GATTTCCTCA 
GGACTCCAGA TTATCTACAA CACTGATGAG GTCTCTTTTA TTCAGAACCT TGTATTTTGT 
GTAGAAAGAG TTTACCGGGT GCCTGACTTC GGTGTCTGGG AAAGAGGAAG CAAATATAAT 
AATGGCAGCA CAGAGCTGCA TTCGAGCTCT GTTGGCTTAG CAAAAGCAGC TCTAGAAGCA 
ATTAATGGAT TCAACCTCTT TGGCAACCAG GGCTGTTCTT GGTCAGTTAT ATTTGTGGAT 
CTCGATGCCC ACAATCGCAA CCGGCAAACT TTGTGCTCGT TGTTACCCAG AGAATCAAGA 
TCTCATAACA CAGATGCTGC CCTGCTCCCC TGCATCAGTT ATCCTGCGTT TGCCTTGGAT 
GATGAAGTTC TCTTTAGCCA GACGCTTGAT AAAGTGATTA GAAAATTAAA AGGAAAATAT 
GGATTTAAGC GTTTCTTGAG AGATGGATAT AGAACATCAT TGGAAGATCC CAATAGACGC 
TACTACAAAC CAGCTGAAAT TAAGCTGTTT GATGGCATTG AATGTGAATT TCCCATATTT 
TTCCTTTACA TGATGATTGA TGGTGTTTTT AGAGGCAATC CCAAGCAAGT AAAAGAGTAT 
CAGGATCTTC TGACTCCAGT ACTTCATCAC ACCACAGAAG GTTATCCTGT CATACCAAAG 
TACTATTATG TGCCAGCTGA TTTTGTAGAG TATGAAAAAA GAAACCCTGG TAGTCAGAAG 
CGATTCCCTA GCAACTGTGG CCGTGATGGA AAACTGTTTC TTTGGGGACA AGCCCTTTAT 
GTCATTGCAA AACTCCTGGC TGATGAATTA ATTAGTCCTA AAGACATTGA TCCTGTTCAG 
CGTTTTGTCC CTCTACAGAA TCAACGAAAT GTGAGCATGA GGTTTTCCAA TCAGGGTCCA 
CTGGAAAATG ATTTGGTTGT TTATGTAGCA CTCATAGCAG AAAGCCAACG CCTTCAAGTT 
TTCCTCAACA CATATGGTAT TCAGACTCAA ACTCCTCAAC AGGTGGAACC CATTCAGATC 
TGGGCCCAGC AGGAGCTGGT GAAAGCTTAT TTCCATCTGG GTATCAATGA AAAATTAGGA 
CTCTGCGGAA GGCCAGATAG ACCTATTGGC TGCCTTGGTA CATCAAAGAT TTATCGCATT 
CTAGGAAAGA CTGTGGTTTG TTACCCTATC ATCTTTGACC TTAGTGATTT CTACATGTCC 
CAAGATGTTT TGCTGCTGAT AGATGACATA AAGAATGCAC TCCAGTTTAT TAAGCAATAC 
TGGAAAATGC ATGGACGTCC ACTTTTCCTT GTTCTCATCC GGGAAGACAA TATAAGAGGT 
AGCCGATTCA ATCCCATATT AGATATGCTG GCAGCATTTA AGAAAGGAAT GGTTGGAGGA 
GTCAAAGTTC ATGTTGATCG TCTACAGACA CTAATATCTG GAGCTGTGGT TGAACAACTT 
GACTTCTTAC GAATCAGTGA AATAGAAGAG CTTCCAGAAT TTAAGAGTTT TGAGGAATTA 
GAAGTTCCCA AACATTCAAA AGTCAAACGA CAAAGCAGCA CCTCCAATGC TCCCGAACAG 
GAACAGAAGC CAGATGTTAC CGTGACTGAA TGGAGAAACA AACCCACCCA TGAAATTCTT 
CAAAAACTGA ATGACTGTAG TTGTCTGGCT AGCCAAGCCA TCCTACTGGG TATACTGCTC 
AAAAGAGAAG GCCCCAACTT CATCACAAAG GACGGTACTG TTTCTGATCA CATTGAGAGA 
GTCTATAGAA GAGCTGGCAG CAAAAAACTT TGGTCGGTGG TGCGCCATGC AGCAAGTCTT 
TTAAGTAAAC TAGTGGACAG CCTGGCCCCA TCCATTACTA ATGTTTTAGT GCAGGGCAAA 
CAGGTAACTC TGGGTGCCTT TGGGCATGAA GAAGAAGTTA TCTCTAATCC TTTGTCTCCG 
GGAGTGATTA AGAACATCAT CTATTACAAG TGTAATACCC ATGATGAGAG GGAGGCAGTA 
ATTCAGCAAG AACTGGTCAT CCACATTGGC TGGATCATCT CCAATAACCC AGAATTATTC 
AGTGGCATGC TGAAAATACG AATTGGGTGG ATCATCCATG CTATGGAGTA TGAACTACAG 
ATCCGTGGTG GAAACAAGCC AGCCAGAGAC TTATATCAGC TGTCACCCAG TGAAGTAAAA 
CAGCTTCTAT TGGATATTCT CCAGCCTCAA CAGAATGGAA GATGTTGGCT GAACAGGCGG 
CAGATCGATG GCTCTTTGAA TAGAACCCCC CCTGGGTTCT ATGACAGAGT ATGGCAGATC 
CTGGAACGCA CACCCAATGG AATCATTGTA GCCGGGAGGC ATTTGCCCCA GCAACCAACC 
CTGTCAGATA TGACCATGTA TGAGATGAAT TTCTCTCTCC TTGTTGAAGA CATGTTGGGA 
AATATTGACC AGCCAAAATA CAGACAGATT GTCGTGGAGT TACTAATGGT CGTATCCATT 
GTACTGGAAA GAAACCCTGA GCTAGAATTT CAAGACAAAG TAGATCTTGA CAAACTGGTG 
AAAGAAGCAT TTCATGAATT TCAAAAAGAT GAGAGTCGAC TAAAGGAAAT TGAAAAACAA 
GATGACATGA CTTCCTTTTA CAACACTCCC CCTCTGGGAA AAAGAGGAAC ATGCAGCTAT 
TTGACAAAGG TCGTGATGAA TCTGCTGCTG GAAGGAGAAG TCAAGCCAAG CAATGATGAC 
CCGTGTCTGG TTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025125587.1
CDS	186..3443
Translation	MASSPDPVVPSPSAFLRSGSVYEPLKSINLPRPDETLWDKLDHYYRIVKSTLLLYQSPTTGLFPTKTCGNDQKAKVHDSLYCAAGAWALALAYRRIDDDKGRTHELEHSAIKCMRGILYCYMRQADKVQQFKQDPRPTTCLHSIFNVHTGDEILSYEEYGHLQINAVSLYLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERVYRVPDFGVWERGSKYNNGSTELHSSSVGLAKAALEAINGFNLFGNQGCSWSVIFVDLDAHNRNRQTLCSLLPRESRSHNTDAALLPCISYPAFALDDEVLFSQTLDKVIRKLKGKYGFKRFLRDGYRTSLEDPNRRYYKPAEIKLFDGIECEFPIFFLYMMIDGVFRGNPKQVKEYQDLLTPVLHHTTEGYPVIPKYYYVPADFVEYEKRNPGSQKRFPSNCGRDGKLFLWGQALYVIAKLLADELISPKDIDPVQRFVPLQNQRNVSMRFSNQGPLENDLVVYVALIAESQRLQVFLNTYGIQTQTPQQVEPIQIWAQQELVKAYFHLGINEKLGLCGRPDRPIGCLGTSKIYRILGKTVVCYPIIFDLSDFYMSQDVLLLIDDIKNALQFIKQYWKMHGRPLFLVLIREDNIRGSRFNPILDMLAAFKKGMVGGVKVHVDRLQTLISGAVVEQLDFLRISEIEELPEFKSFEELEVPKHSKVKRQSSTSNAPEQEQKPDVTVTEWRNKPTHEILQKLNDCSCLASQAILLGILLKREGPNFITKDGTVSDHIERVYRRAGSKKLWSVVRHAASLLSKLVDSLAPSITNVLVQGKQVTLGAFGHEEEVISNPLSPGVIKNIIYYKCNTHDEREAVIQQELVIHIGWIISNNPELFSGMLKIRIGWIIHAMEYELQIRGGNKPARDLYQLSPSEVKQLLLDILQPQQNGRCWLNRRQIDGSLNRTPPGFYDRVWQILERTPNGIIVAGRHLPQQPTLSDMTMYEMNFSLLVEDMLGNIDQPKYRQIVVELLMVVSIVLERNPELEFQDKVDLDKLVKEAFHEFQKDESRLKEIEKQDDMTSFYNTPPLGKRGTCSYLTKVVMNLLLEGEVKPSNDDPCLVS

Target ORF information:

RefSeq Version	XM_025269802.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis phosphorylase kinase regulatory subunit beta (PHKB), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025269802.1

ORF Insert Sequence:

ATGGCCAGCT CACCTGATCC AGTCGTCCCT TCTCCATCAG CTTTCTTAAG GTCTGGCTCA 
GTTTATGAAC CTCTGAAAAG CATTAATCTT CCGAGACCTG ATGAAACTCT GTGGGATAAA 
CTGGATCACT ATTACAGAAT TGTCAAGTCA ACATTGCTGC TGTATCAAAG TCCAACCACA 
GGTCTCTTTC CCACTAAAAC ATGTGGTAAT GATCAGAAGG CCAAGGTTCA TGACAGTCTG 
TACTGTGCTG CTGGTGCCTG GGCTTTGGCT CTTGCATACA GGCGCATTGA TGATGACAAG 
GGAAGGACCC ATGAGCTGGA GCACTCGGCT ATAAAATGTA TGAGAGGAAT TCTCTACTGC 
TACATGCGTC AGGCCGATAA GGTCCAGCAA TTTAAGCAGG ATCCACGCCC AACAACATGT 
CTTCACTCCA TTTTCAATGT ACATACAGGA GATGAGATCC TCTCCTATGA GGAATATGGT 
CATCTTCAGA TAAATGCAGT GTCCCTCTAT CTCCTTTACC TTGTCGAAAT GATTTCCTCA 
GGACTCCAGA TTATCTACAA CACTGATGAG GTCTCTTTTA TTCAGAACCT TGTATTTTGT 
GTAGAAAGAG TTTACCGGGT GCCTGACTTC GGTGTCTGGG AAAGAGGAAG CAAATATAAT 
AATGGCAGCA CAGAGCTGCA TTCGAGCTCT GTTGGCTTAG CAAAAGCAGC TCTAGAAGCA 
ATTAATGGAT TCAACCTCTT TGGCAACCAG GGCTGTTCTT GGTCAGTTAT ATTTGTGGAT 
CTCGATGCCC ACAATCGCAA CCGGCAAACT TTGTGCTCGT TGTTACCCAG AGAATCAAGA 
TCTCATAACA CAGATGCTGC CCTGCTCCCC TGCATCAGTT ATCCTGCGTT TGCCTTGGAT 
GATGAAGTTC TCTTTAGCCA GACGCTTGAT AAAGTGATTA GAAAATTAAA AGGAAAATAT 
GGATTTAAGC GTTTCTTGAG AGATGGATAT AGAACATCAT TGGAAGATCC CAATAGACGC 
TACTACAAAC CAGCTGAAAT TAAGCTGTTT GATGGCATTG AATGTGAATT TCCCATATTT 
TTCCTTTACA TGATGATTGA TGGTGTTTTT AGAGGCAATC CCAAGCAAGT AAAAGAGTAT 
CAGGATCTTC TGACTCCAGT ACTTCATCAC ACCACAGAAG GTTATCCTGT CATACCAAAG 
TACTATTATG TGCCAGCTGA TTTTGTAGAG TATGAAAAAA GAAACCCTGG TAGTCAGAAG 
CGATTCCCTA GCAACTGTGG CCGTGATGGA AAACTGTTTC TTTGGGGACA AGCCCTTTAT 
GTCATTGCAA AACTCCTGGC TGATGAATTA ATTAGTCCTA AAGACATTGA TCCTGTTCAG 
CGTTTTGTCC CTCTACAGAA TCAACGAAAT GTGAGCATGA GGTTTTCCAA TCAGGGTCCA 
CTGGAAAATG ATTTGGTTGT TTATGTAGCA CTCATAGCAG AAAGCCAACG CCTTCAAGTT 
TTCCTCAACA CATATGGTAT TCAGACTCAA ACTCCTCAAC AGGTGGAACC CATTCAGATC 
TGGGCCCAGC AGGAGCTGGT GAAAGCTTAT TTCCATCTGG GTATCAATGA AAAATTAGGA 
CTCTGCGGAA GGCCAGATAG ACCTATTGGC TGCCTTGGTA CATCAAAGAT TTATCGCATT 
CTAGGAAAGA CTGTGGTTTG TTACCCTATC ATCTTTGACC TTAGTGATTT CTACATGTCC 
CAAGATGTTT TGCTGCTGAT AGATGACATA AAGAATGCAC TCCAGTTTAT TAAGCAATAC 
TGGAAAATGC ATGGACGTCC ACTTTTCCTT GTTCTCATCC GGGAAGACAA TATAAGAGGT 
AGCCGATTCA ATCCCATATT AGATATGCTG GCAGCATTTA AGAAAGGAAT GGTTGGAGGA 
GTCAAAGTTC ATGTTGATCG TCTACAGACA CTAATATCTG GAGCTGTGGT TGAACAACTT 
GACTTCTTAC GAATCAGTGA AATAGAAGAG CTTCCAGAAT TTAAGAGTTT TGAGGAATTA 
GAAGTTCCCA AACATTCAAA AGTCAAACGA CAAAGCAGCA CCTCCAATGC TCCCGAACAG 
GAACAGAAGC CAGATGTTAC CGTGACTGAA TGGAGAAACA AACCCACCCA TGAAATTCTT 
CAAAAACTGA ATGACTGTAG TTGTCTGGCT AGCCAAGCCA TCCTACTGGG TATACTGCTC 
AAAAGAGAAG GCCCCAACTT CATCACAAAG GACGGTACTG TTTCTGATCA CATTGAGAGA 
GTCTATAGAA GAGCTGGCAG CAAAAAACTT TGGTCGGTGG TGCGCCATGC AGCAAGTCTT 
TTAAGTAAAC TAGTGGACAG CCTGGCCCCA TCCATTACTA ATGTTTTAGT GCAGGGCAAA 
CAGGTAACTC TGGGTGCCTT TGGGCATGAA GAAGAAGTTA TCTCTAATCC TTTGTCTCCG 
GGAGTGATTA AGAACATCAT CTATTACAAG TGTAATACCC ATGATGAGAG GGAGGCAGTA 
ATTCAGCAAG AACTGGTCAT CCACATTGGC TGGATCATCT CCAATAACCC AGAATTATTC 
AGTGGCATGC TGAAAATACG AATTGGGTGG ATCATCCATG CTATGGAGTA TGAACTACAG 
ATCCGTGGTG GAAACAAGCC AGCCAGAGAC TTATATCAGC TGTCACCCAG TGAAGTAAAA 
CAGCTTCTAT TGGATATTCT CCAGCCTCAA CAGAATGGAA GATGTTGGCT GAACAGGCGG 
CAGATCGATG GCTCTTTGAA TAGAACCCCC CCTGGGTTCT ATGACAGAGT ATGGCAGATC 
CTGGAACGCA CACCCAATGG AATCATTGTA GCCGGGAGGC ATTTGCCCCA GCAACCAACC 
CTGTCAGATA TGACCATGTA TGAGATGAAT TTCTCTCTCC TTGTTGAAGA CATGTTGGGA 
AATATTGACC AGCCAAAATA CAGACAGATT GTCGTGGAGT TACTAATGGT CGTATCCATT 
GTACTGGAAA GAAACCCTGA GCTAGAATTT CAAGACAAAG TAGATCTTGA CAAACTGGTG 
AAAGAAGCAT TTCATGAATT TCAAAAAGAT GAGAGTCGAC TAAAGGAAAT TGAAAAACAA 
GATGACATGA CTTCCTTTTA CAACACTCCC CCTCTGGGAA AAAGAGGAAC ATGCAGCTAT 
TTGACAAAGG TCGTGATGAA TCTGCTGCTG GAAGGAGAAG TCAAGCCAAG CAATGATGAC 
CCGTGTCTGG TTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKB ( XM_025269804.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025125589.1 Bubalus bubalis phosphorylase kinase regulatory subunit beta (PHKB), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBu53306
Clone ID Related Accession (Same CDS sequence)	XM_025269804.1
Accession Version	XM_025269804.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	phosphorylase b kinase regulatory subunit beta isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037562.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT CACCTGATCC AGTCGTCCCT TCTCCATCAG CTTTCTTAAG GTCTGATGAG 
AAGAACTGGC TCAGAGAGTG CCCAAGTGTA GAAAGCTGTG GACATAGCTG TGACTGTAGC 
CGAGGCTCAG TTTATGAACC TCTGAAAAGC ATTAATCTTC CGAGACCTGA TGAAACTCTG 
TGGGATAAAC TGGATCACTA TTACAGAATT GTCAAGTCAA CATTGCTGCT GTATCAAAGT 
CCAACCACAG GTCTCTTTCC CACTAAAACA TGTGGTAATG ATCAGAAGGC CAAGGTTCAT 
GACAGTCTGT ACTGTGCTGC TGGTGCCTGG GCTTTGGCTC TTGCATACAG GCGCATTGAT 
GATGACAAGG GAAGGACCCA TGAGCTGGAG CACTCGGCTA TAAAATGTAT GAGAGGAATT 
CTCTACTGCT ACATGCGTCA GGCCGATAAG GTCCAGCAAT TTAAGCAGGA TCCACGCCCA 
ACAACATGTC TTCACTCCAT TTTCAATGTA CATACAGGAG ATGAGATCCT CTCCTATGAG 
GAATATGGTC ATCTTCAGAT AAATGCAGTG TCCCTCTATC TCCTTTACCT TGTCGAAATG 
ATTTCCTCAG GACTCCAGAT TATCTACAAC ACTGATGAGG TCTCTTTTAT TCAGAACCTT 
GTATTTTGTG TAGAAAGAGT TTACCGGGTG CCTGACTTCG GTGTCTGGGA AAGAGGAAGC 
AAATATAATA ATGGCAGCAC AGAGCTGCAT TCGAGCTCTG TTGGCTTAGC AAAAGCAGCT 
CTAGAAGCAA TTAATGGATT CAACCTCTTT GGCAACCAGG GCTGTTCTTG GTCAGTTATA 
TTTGTGGATC TCGATGCCCA CAATCGCAAC CGGCAAACTT TGTGCTCGTT GTTACCCAGA 
GAATCAAGAT CTCATAACAC AGATGCTGCC CTGCTCCCCT GCATCAGTTA TCCTGCGTTT 
GCCTTGGATG ATGAAGTTCT CTTTAGCCAG ACGCTTGATA AAGTGATTAG AAAATTAAAA 
GGAAAATATG GATTTAAGCG TTTCTTGAGA GATGGATATA GAACATCATT GGAAGATCCC 
AATAGACGCT ACTACAAACC AGCTGAAATT AAGCTGTTTG ATGGCATTGA ATGTGAATTT 
CCCATATTTT TCCTTTACAT GATGATTGAT GGTGTTTTTA GAGGCAATCC CAAGCAAGTA 
AAAGAGTATC AGGATCTTCT GACTCCAGTA CTTCATCACA CCACAGAAGG TTATCCTGTC 
ATACCAAAGT ACTATTATGT GCCAGCTGAT TTTGTAGAGT ATGAAAAAAG AAACCCTGGT 
AGTCAGAAGC GATTCCCTAG CAACTGTGGC CGTGATGGAA AACTGTTTCT TTGGGGACAA 
GCCCTTTATG TCATTGCAAA ACTCCTGGCT GATGAATTAA TTAGTCCTAA AGACATTGAT 
CCTGTTCAGC GTTTTGTCCC TCTACAGAAT CAACGAAATG TGAGCATGAG GTTTTCCAAT 
CAGGGTCCAC TGGAAAATGA TTTGGTTGTT TATGTAGCAC TCATAGCAGA AAGCCAACGC 
CTTCAAGTTT TCCTCAACAC ATATGGTATT CAGACTCAAA CTCCTCAACA GGTGGAACCC 
ATTCAGATCT GGGCCCAGCA GGAGCTGGTG AAAGCTTATT TCCATCTGGG TATCAATGAA 
AAATTAGGAC TCTGCGGAAG GCCAGATAGA CCTATTGGCT GCCTTGGTAC ATCAAAGATT 
TATCGCATTC TAGGAAAGAC TGTGGTTTGT TACCCTATCA TCTTTGACCT TAGTGATTTC 
TACATGTCCC AAGATGTTTT GCTGCTGATA GATGACATAA AGAATGCACT CCAGTTTATT 
AAGCAATACT GGAAAATGCA TGGACGTCCA CTTTTCCTTG TTCTCATCCG GGAAGACAAT 
ATAAGAGGTA GCCGATTCAA TCCCATATTA GATATGCTGG CAGCATTTAA GAAAGGAATG 
GTTGGAGGAG TCAAAGTTCA TGTTGATCGT CTACAGACAC TAATATCTGG AGCTGTGGTT 
GAACAACTTG ACTTCTTACG AATCAGTGAA ATAGAAGAGC TTCCAGAATT TAAGAGTTTT 
GAGGAATTAG AAGTTCCCAA ACATTCAAAA GTCAAACGAC AAAGCAGCAC CTCCAATGCT 
CCCGAACAGG AACAGAAGCC AGATGTTACC GTGACTGAAT GGAGAAACAA ACCCACCCAT 
GAAATTCTTC AAAAACTGAA TGACTGTAGT TGTCTGGCTA GCCAAGCCAT CCTACTGGGT 
ATACTGCTCA AAAGAGAAGG CCCCAACTTC ATCACAAAGG ACGGTACTGT TTCTGATCAC 
ATTGAGAGAG TCTATAGAAG AGCTGGCAGC AAAAAACTTT GGTGGATCAT CCATGCTATG 
GAGTATGAAC TACAGATCCG TGGTGGAAAC AAGCCAGCCA GAGACTTATA TCAGCTGTCA 
CCCAGTGAAG TAAAACAGCT TCTATTGGAT ATTCTCCAGC CTCAACAGAA TGGAAGATGT 
TGGCTGAACA GGCGGCAGAT CGATGGCTCT TTGAATAGAA CCCCCCCTGG GTTCTATGAC 
AGAGTATGGC AGATCCTGGA ACGCACACCC AATGGAATCA TTGTAGCCGG GAGGCATTTG 
CCCCAGCAAC CAACCCTGTC AGATATGACC ATGTATGAGA TGAATTTCTC TCTCCTTGTT 
GAAGACATGT TGGGAAATAT TGACCAGCCA AAATACAGAC AGATTGTCGT GGAGTTACTA 
ATGGTCGTAT CCATTGTACT GGAAAGAAAC CCTGAGCTAG AATTTCAAGA CAAAGTAGAT 
CTTGACAAAC TGGTGAAAGA AGCATTTCAT GAATTTCAAA AAGATGAGAG TCGACTAAAG 
GAAATTGAAA AACAAGATGA CATGACTTCC TTTTACAACA CTCCCCCTCT GGGAAAAAGA 
GGAACATGCA GCTATTTGAC AAAGGTCGTG ATGAATCTGC TGCTGGAAGG AGAAGTCAAG 
CCAAGCAATG ATGACCCGTG TCTGGTTAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025125589.1
CDS	186..3218
Translation	MASSPDPVVPSPSAFLRSDEKNWLRECPSVESCGHSCDCSRGSVYEPLKSINLPRPDETLWDKLDHYYRIVKSTLLLYQSPTTGLFPTKTCGNDQKAKVHDSLYCAAGAWALALAYRRIDDDKGRTHELEHSAIKCMRGILYCYMRQADKVQQFKQDPRPTTCLHSIFNVHTGDEILSYEEYGHLQINAVSLYLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERVYRVPDFGVWERGSKYNNGSTELHSSSVGLAKAALEAINGFNLFGNQGCSWSVIFVDLDAHNRNRQTLCSLLPRESRSHNTDAALLPCISYPAFALDDEVLFSQTLDKVIRKLKGKYGFKRFLRDGYRTSLEDPNRRYYKPAEIKLFDGIECEFPIFFLYMMIDGVFRGNPKQVKEYQDLLTPVLHHTTEGYPVIPKYYYVPADFVEYEKRNPGSQKRFPSNCGRDGKLFLWGQALYVIAKLLADELISPKDIDPVQRFVPLQNQRNVSMRFSNQGPLENDLVVYVALIAESQRLQVFLNTYGIQTQTPQQVEPIQIWAQQELVKAYFHLGINEKLGLCGRPDRPIGCLGTSKIYRILGKTVVCYPIIFDLSDFYMSQDVLLLIDDIKNALQFIKQYWKMHGRPLFLVLIREDNIRGSRFNPILDMLAAFKKGMVGGVKVHVDRLQTLISGAVVEQLDFLRISEIEELPEFKSFEELEVPKHSKVKRQSSTSNAPEQEQKPDVTVTEWRNKPTHEILQKLNDCSCLASQAILLGILLKREGPNFITKDGTVSDHIERVYRRAGSKKLWWIIHAMEYELQIRGGNKPARDLYQLSPSEVKQLLLDILQPQQNGRCWLNRRQIDGSLNRTPPGFYDRVWQILERTPNGIIVAGRHLPQQPTLSDMTMYEMNFSLLVEDMLGNIDQPKYRQIVVELLMVVSIVLERNPELEFQDKVDLDKLVKEAFHEFQKDESRLKEIEKQDDMTSFYNTPPLGKRGTCSYLTKVVMNLLLEGEVKPSNDDPCLVS

Target ORF information:

RefSeq Version	XM_025269804.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis phosphorylase kinase regulatory subunit beta (PHKB), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025269804.1

ORF Insert Sequence:

ATGGCCAGCT CACCTGATCC AGTCGTCCCT TCTCCATCAG CTTTCTTAAG GTCTGATGAG 
AAGAACTGGC TCAGAGAGTG CCCAAGTGTA GAAAGCTGTG GACATAGCTG TGACTGTAGC 
CGAGGCTCAG TTTATGAACC TCTGAAAAGC ATTAATCTTC CGAGACCTGA TGAAACTCTG 
TGGGATAAAC TGGATCACTA TTACAGAATT GTCAAGTCAA CATTGCTGCT GTATCAAAGT 
CCAACCACAG GTCTCTTTCC CACTAAAACA TGTGGTAATG ATCAGAAGGC CAAGGTTCAT 
GACAGTCTGT ACTGTGCTGC TGGTGCCTGG GCTTTGGCTC TTGCATACAG GCGCATTGAT 
GATGACAAGG GAAGGACCCA TGAGCTGGAG CACTCGGCTA TAAAATGTAT GAGAGGAATT 
CTCTACTGCT ACATGCGTCA GGCCGATAAG GTCCAGCAAT TTAAGCAGGA TCCACGCCCA 
ACAACATGTC TTCACTCCAT TTTCAATGTA CATACAGGAG ATGAGATCCT CTCCTATGAG 
GAATATGGTC ATCTTCAGAT AAATGCAGTG TCCCTCTATC TCCTTTACCT TGTCGAAATG 
ATTTCCTCAG GACTCCAGAT TATCTACAAC ACTGATGAGG TCTCTTTTAT TCAGAACCTT 
GTATTTTGTG TAGAAAGAGT TTACCGGGTG CCTGACTTCG GTGTCTGGGA AAGAGGAAGC 
AAATATAATA ATGGCAGCAC AGAGCTGCAT TCGAGCTCTG TTGGCTTAGC AAAAGCAGCT 
CTAGAAGCAA TTAATGGATT CAACCTCTTT GGCAACCAGG GCTGTTCTTG GTCAGTTATA 
TTTGTGGATC TCGATGCCCA CAATCGCAAC CGGCAAACTT TGTGCTCGTT GTTACCCAGA 
GAATCAAGAT CTCATAACAC AGATGCTGCC CTGCTCCCCT GCATCAGTTA TCCTGCGTTT 
GCCTTGGATG ATGAAGTTCT CTTTAGCCAG ACGCTTGATA AAGTGATTAG AAAATTAAAA 
GGAAAATATG GATTTAAGCG TTTCTTGAGA GATGGATATA GAACATCATT GGAAGATCCC 
AATAGACGCT ACTACAAACC AGCTGAAATT AAGCTGTTTG ATGGCATTGA ATGTGAATTT 
CCCATATTTT TCCTTTACAT GATGATTGAT GGTGTTTTTA GAGGCAATCC CAAGCAAGTA 
AAAGAGTATC AGGATCTTCT GACTCCAGTA CTTCATCACA CCACAGAAGG TTATCCTGTC 
ATACCAAAGT ACTATTATGT GCCAGCTGAT TTTGTAGAGT ATGAAAAAAG AAACCCTGGT 
AGTCAGAAGC GATTCCCTAG CAACTGTGGC CGTGATGGAA AACTGTTTCT TTGGGGACAA 
GCCCTTTATG TCATTGCAAA ACTCCTGGCT GATGAATTAA TTAGTCCTAA AGACATTGAT 
CCTGTTCAGC GTTTTGTCCC TCTACAGAAT CAACGAAATG TGAGCATGAG GTTTTCCAAT 
CAGGGTCCAC TGGAAAATGA TTTGGTTGTT TATGTAGCAC TCATAGCAGA AAGCCAACGC 
CTTCAAGTTT TCCTCAACAC ATATGGTATT CAGACTCAAA CTCCTCAACA GGTGGAACCC 
ATTCAGATCT GGGCCCAGCA GGAGCTGGTG AAAGCTTATT TCCATCTGGG TATCAATGAA 
AAATTAGGAC TCTGCGGAAG GCCAGATAGA CCTATTGGCT GCCTTGGTAC ATCAAAGATT 
TATCGCATTC TAGGAAAGAC TGTGGTTTGT TACCCTATCA TCTTTGACCT TAGTGATTTC 
TACATGTCCC AAGATGTTTT GCTGCTGATA GATGACATAA AGAATGCACT CCAGTTTATT 
AAGCAATACT GGAAAATGCA TGGACGTCCA CTTTTCCTTG TTCTCATCCG GGAAGACAAT 
ATAAGAGGTA GCCGATTCAA TCCCATATTA GATATGCTGG CAGCATTTAA GAAAGGAATG 
GTTGGAGGAG TCAAAGTTCA TGTTGATCGT CTACAGACAC TAATATCTGG AGCTGTGGTT 
GAACAACTTG ACTTCTTACG AATCAGTGAA ATAGAAGAGC TTCCAGAATT TAAGAGTTTT 
GAGGAATTAG AAGTTCCCAA ACATTCAAAA GTCAAACGAC AAAGCAGCAC CTCCAATGCT 
CCCGAACAGG AACAGAAGCC AGATGTTACC GTGACTGAAT GGAGAAACAA ACCCACCCAT 
GAAATTCTTC AAAAACTGAA TGACTGTAGT TGTCTGGCTA GCCAAGCCAT CCTACTGGGT 
ATACTGCTCA AAAGAGAAGG CCCCAACTTC ATCACAAAGG ACGGTACTGT TTCTGATCAC 
ATTGAGAGAG TCTATAGAAG AGCTGGCAGC AAAAAACTTT GGTGGATCAT CCATGCTATG 
GAGTATGAAC TACAGATCCG TGGTGGAAAC AAGCCAGCCA GAGACTTATA TCAGCTGTCA 
CCCAGTGAAG TAAAACAGCT TCTATTGGAT ATTCTCCAGC CTCAACAGAA TGGAAGATGT 
TGGCTGAACA GGCGGCAGAT CGATGGCTCT TTGAATAGAA CCCCCCCTGG GTTCTATGAC 
AGAGTATGGC AGATCCTGGA ACGCACACCC AATGGAATCA TTGTAGCCGG GAGGCATTTG 
CCCCAGCAAC CAACCCTGTC AGATATGACC ATGTATGAGA TGAATTTCTC TCTCCTTGTT 
GAAGACATGT TGGGAAATAT TGACCAGCCA AAATACAGAC AGATTGTCGT GGAGTTACTA 
ATGGTCGTAT CCATTGTACT GGAAAGAAAC CCTGAGCTAG AATTTCAAGA CAAAGTAGAT 
CTTGACAAAC TGGTGAAAGA AGCATTTCAT GAATTTCAAA AAGATGAGAG TCGACTAAAG 
GAAATTGAAA AACAAGATGA CATGACTTCC TTTTACAACA CTCCCCCTCT GGGAAAAAGA 
GGAACATGCA GCTATTTGAC AAAGGTCGTG ATGAATCTGC TGCTGGAAGG AGAAGTCAAG 
CCAAGCAATG ATGACCCGTG TCTGGTTAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKB ( XM_025269805.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025125590.1 Bubalus bubalis phosphorylase kinase regulatory subunit beta (PHKB), transcript variant X9, mRNA.

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CloneID	OBu53307
Clone ID Related Accession (Same CDS sequence)	XM_025269805.1
Accession Version	XM_025269805.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3285bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	phosphorylase b kinase regulatory subunit beta isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037562.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGC TCATGGCCGA AGTAAGCTGG AAGGTCTTGG AGCGAAGAGC CCGGACCAAG 
CGCTCAGCTT TCTTAAGGTC TGGCTCAGTT TATGAACCTC TGAAAAGCAT TAATCTTCCG 
AGACCTGATG AAACTCTGTG GGATAAACTG GATCACTATT ACAGAATTGT CAAGTCAACA 
TTGCTGCTGT ATCAAAGTCC AACCACAGGT CTCTTTCCCA CTAAAACATG TGGTAATGAT 
CAGAAGGCCA AGGTTCATGA CAGTCTGTAC TGTGCTGCTG GTGCCTGGGC TTTGGCTCTT 
GCATACAGGC GCATTGATGA TGACAAGGGA AGGACCCATG AGCTGGAGCA CTCGGCTATA 
AAATGTATGA GAGGAATTCT CTACTGCTAC ATGCGTCAGG CCGATAAGGT CCAGCAATTT 
AAGCAGGATC CACGCCCAAC AACATGTCTT CACTCCATTT TCAATGTACA TACAGGAGAT 
GAGATCCTCT CCTATGAGGA ATATGGTCAT CTTCAGATAA ATGCAGTGTC CCTCTATCTC 
CTTTACCTTG TCGAAATGAT TTCCTCAGGA CTCCAGATTA TCTACAACAC TGATGAGGTC 
TCTTTTATTC AGAACCTTGT ATTTTGTGTA GAAAGAGTTT ACCGGGTGCC TGACTTCGGT 
GTCTGGGAAA GAGGAAGCAA ATATAATAAT GGCAGCACAG AGCTGCATTC GAGCTCTGTT 
GGCTTAGCAA AAGCAGCTCT AGAAGCAATT AATGGATTCA ACCTCTTTGG CAACCAGGGC 
TGTTCTTGGT CAGTTATATT TGTGGATCTC GATGCCCACA ATCGCAACCG GCAAACTTTG 
TGCTCGTTGT TACCCAGAGA ATCAAGATCT CATAACACAG ATGCTGCCCT GCTCCCCTGC 
ATCAGTTATC CTGCGTTTGC CTTGGATGAT GAAGTTCTCT TTAGCCAGAC GCTTGATAAA 
GTGATTAGAA AATTAAAAGG AAAATATGGA TTTAAGCGTT TCTTGAGAGA TGGATATAGA 
ACATCATTGG AAGATCCCAA TAGACGCTAC TACAAACCAG CTGAAATTAA GCTGTTTGAT 
GGCATTGAAT GTGAATTTCC CATATTTTTC CTTTACATGA TGATTGATGG TGTTTTTAGA 
GGCAATCCCA AGCAAGTAAA AGAGTATCAG GATCTTCTGA CTCCAGTACT TCATCACACC 
ACAGAAGGTT ATCCTGTCAT ACCAAAGTAC TATTATGTGC CAGCTGATTT TGTAGAGTAT 
GAAAAAAGAA ACCCTGGTAG TCAGAAGCGA TTCCCTAGCA ACTGTGGCCG TGATGGAAAA 
CTGTTTCTTT GGGGACAAGC CCTTTATGTC ATTGCAAAAC TCCTGGCTGA TGAATTAATT 
AGTCCTAAAG ACATTGATCC TGTTCAGCGT TTTGTCCCTC TACAGAATCA ACGAAATGTG 
AGCATGAGGT TTTCCAATCA GGGTCCACTG GAAAATGATT TGGTTGTTTA TGTAGCACTC 
ATAGCAGAAA GCCAACGCCT TCAAGTTTTC CTCAACACAT ATGGTATTCA GACTCAAACT 
CCTCAACAGG TGGAACCCAT TCAGATCTGG GCCCAGCAGG AGCTGGTGAA AGCTTATTTC 
CATCTGGGTA TCAATGAAAA ATTAGGACTC TGCGGAAGGC CAGATAGACC TATTGGCTGC 
CTTGGTACAT CAAAGATTTA TCGCATTCTA GGAAAGACTG TGGTTTGTTA CCCTATCATC 
TTTGACCTTA GTGATTTCTA CATGTCCCAA GATGTTTTGC TGCTGATAGA TGACATAAAG 
AATGCACTCC AGTTTATTAA GCAATACTGG AAAATGCATG GACGTCCACT TTTCCTTGTT 
CTCATCCGGG AAGACAATAT AAGAGGTAGC CGATTCAATC CCATATTAGA TATGCTGGCA 
GCATTTAAGA AAGGAATGGT TGGAGGAGTC AAAGTTCATG TTGATCGTCT ACAGACACTA 
ATATCTGGAG CTGTGGTTGA ACAACTTGAC TTCTTACGAA TCAGTGAAAT AGAAGAGCTT 
CCAGAATTTA AGAGTTTTGA GGAATTAGAA GTTCCCAAAC ATTCAAAAGT CAAACGACAA 
AGCAGCACCT CCAATGCTCC CGAACAGGAA CAGAAGCCAG ATGTTACCGT GACTGAATGG 
AGAAACAAAC CCACCCATGA AATTCTTCAA AAACTGAATG ACTGTAGTTG TCTGGCTAGC 
CAAGCCATCC TACTGGGTAT ACTGCTCAAA AGAGAAGGCC CCAACTTCAT CACAAAGGAC 
GGTACTGTTT CTGATCACAT TGAGAGAGTC TATAGAAGAG CTGGCAGCAA AAAACTTTGG 
TCGGTGGTGC GCCATGCAGC AAGTCTTTTA AGTAAACTAG TGGACAGCCT GGCCCCATCC 
ATTACTAATG TTTTAGTGCA GGGCAAACAG GTAACTCTGG GTGCCTTTGG GCATGAAGAA 
GAAGTTATCT CTAATCCTTT GTCTCCGGGA GTGATTAAGA ACATCATCTA TTACAAGTGT 
AATACCCATG ATGAGAGGGA GGCAGTAATT CAGCAAGAAC TGGTCATCCA CATTGGCTGG 
ATCATCTCCA ATAACCCAGA ATTATTCAGT GGCATGCTGA AAATACGAAT TGGGTGGATC 
ATCCATGCTA TGGAGTATGA ACTACAGATC CGTGGTGGAA ACAAGCCAGC CAGAGACTTA 
TATCAGCTGT CACCCAGTGA AGTAAAACAG CTTCTATTGG ATATTCTCCA GCCTCAACAG 
AATGGAAGAT GTTGGCTGAA CAGGCGGCAG ATCGATGGCT CTTTGAATAG AACCCCCCCT 
GGGTTCTATG ACAGAGTATG GCAGATCCTG GAACGCACAC CCAATGGAAT CATTGTAGCC 
GGGAGGCATT TGCCCCAGCA ACCAACCCTG TCAGATATGA CCATGTATGA GATGAATTTC 
TCTCTCCTTG TTGAAGACAT GTTGGGAAAT ATTGACCAGC CAAAATACAG ACAGATTGTC 
GTGGAGTTAC TAATGGTCGT ATCCATTGTA CTGGAAAGAA ACCCTGAGCT AGAATTTCAA 
GACAAAGTAG ATCTTGACAA ACTGGTGAAA GAAGCATTTC ATGAATTTCA AAAAGATGAG 
AGTCGACTAA AGGAAATTGA AAAACAAGAT GACATGACTT CCTTTTACAA CACTCCCCCT 
CTGGGAAAAA GAGGAACATG CAGCTATTTG ACAAAGGTCG TGATGAATCT GCTGCTGGAA 
GGAGAAGTCA AGCCAAGCAA TGATGACCCG TGTCTGGTTA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025125590.1
CDS	108..3392
Translation	MAGLMAEVSWKVLERRARTKRSAFLRSGSVYEPLKSINLPRPDETLWDKLDHYYRIVKSTLLLYQSPTTGLFPTKTCGNDQKAKVHDSLYCAAGAWALALAYRRIDDDKGRTHELEHSAIKCMRGILYCYMRQADKVQQFKQDPRPTTCLHSIFNVHTGDEILSYEEYGHLQINAVSLYLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERVYRVPDFGVWERGSKYNNGSTELHSSSVGLAKAALEAINGFNLFGNQGCSWSVIFVDLDAHNRNRQTLCSLLPRESRSHNTDAALLPCISYPAFALDDEVLFSQTLDKVIRKLKGKYGFKRFLRDGYRTSLEDPNRRYYKPAEIKLFDGIECEFPIFFLYMMIDGVFRGNPKQVKEYQDLLTPVLHHTTEGYPVIPKYYYVPADFVEYEKRNPGSQKRFPSNCGRDGKLFLWGQALYVIAKLLADELISPKDIDPVQRFVPLQNQRNVSMRFSNQGPLENDLVVYVALIAESQRLQVFLNTYGIQTQTPQQVEPIQIWAQQELVKAYFHLGINEKLGLCGRPDRPIGCLGTSKIYRILGKTVVCYPIIFDLSDFYMSQDVLLLIDDIKNALQFIKQYWKMHGRPLFLVLIREDNIRGSRFNPILDMLAAFKKGMVGGVKVHVDRLQTLISGAVVEQLDFLRISEIEELPEFKSFEELEVPKHSKVKRQSSTSNAPEQEQKPDVTVTEWRNKPTHEILQKLNDCSCLASQAILLGILLKREGPNFITKDGTVSDHIERVYRRAGSKKLWSVVRHAASLLSKLVDSLAPSITNVLVQGKQVTLGAFGHEEEVISNPLSPGVIKNIIYYKCNTHDEREAVIQQELVIHIGWIISNNPELFSGMLKIRIGWIIHAMEYELQIRGGNKPARDLYQLSPSEVKQLLLDILQPQQNGRCWLNRRQIDGSLNRTPPGFYDRVWQILERTPNGIIVAGRHLPQQPTLSDMTMYEMNFSLLVEDMLGNIDQPKYRQIVVELLMVVSIVLERNPELEFQDKVDLDKLVKEAFHEFQKDESRLKEIEKQDDMTSFYNTPPLGKRGTCSYLTKVVMNLLLEGEVKPSNDDPCLVS

Target ORF information:

RefSeq Version	XM_025269805.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis phosphorylase kinase regulatory subunit beta (PHKB), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025269805.1

ORF Insert Sequence:

ATGGCGGGGC TCATGGCCGA AGTAAGCTGG AAGGTCTTGG AGCGAAGAGC CCGGACCAAG 
CGCTCAGCTT TCTTAAGGTC TGGCTCAGTT TATGAACCTC TGAAAAGCAT TAATCTTCCG 
AGACCTGATG AAACTCTGTG GGATAAACTG GATCACTATT ACAGAATTGT CAAGTCAACA 
TTGCTGCTGT ATCAAAGTCC AACCACAGGT CTCTTTCCCA CTAAAACATG TGGTAATGAT 
CAGAAGGCCA AGGTTCATGA CAGTCTGTAC TGTGCTGCTG GTGCCTGGGC TTTGGCTCTT 
GCATACAGGC GCATTGATGA TGACAAGGGA AGGACCCATG AGCTGGAGCA CTCGGCTATA 
AAATGTATGA GAGGAATTCT CTACTGCTAC ATGCGTCAGG CCGATAAGGT CCAGCAATTT 
AAGCAGGATC CACGCCCAAC AACATGTCTT CACTCCATTT TCAATGTACA TACAGGAGAT 
GAGATCCTCT CCTATGAGGA ATATGGTCAT CTTCAGATAA ATGCAGTGTC CCTCTATCTC 
CTTTACCTTG TCGAAATGAT TTCCTCAGGA CTCCAGATTA TCTACAACAC TGATGAGGTC 
TCTTTTATTC AGAACCTTGT ATTTTGTGTA GAAAGAGTTT ACCGGGTGCC TGACTTCGGT 
GTCTGGGAAA GAGGAAGCAA ATATAATAAT GGCAGCACAG AGCTGCATTC GAGCTCTGTT 
GGCTTAGCAA AAGCAGCTCT AGAAGCAATT AATGGATTCA ACCTCTTTGG CAACCAGGGC 
TGTTCTTGGT CAGTTATATT TGTGGATCTC GATGCCCACA ATCGCAACCG GCAAACTTTG 
TGCTCGTTGT TACCCAGAGA ATCAAGATCT CATAACACAG ATGCTGCCCT GCTCCCCTGC 
ATCAGTTATC CTGCGTTTGC CTTGGATGAT GAAGTTCTCT TTAGCCAGAC GCTTGATAAA 
GTGATTAGAA AATTAAAAGG AAAATATGGA TTTAAGCGTT TCTTGAGAGA TGGATATAGA 
ACATCATTGG AAGATCCCAA TAGACGCTAC TACAAACCAG CTGAAATTAA GCTGTTTGAT 
GGCATTGAAT GTGAATTTCC CATATTTTTC CTTTACATGA TGATTGATGG TGTTTTTAGA 
GGCAATCCCA AGCAAGTAAA AGAGTATCAG GATCTTCTGA CTCCAGTACT TCATCACACC 
ACAGAAGGTT ATCCTGTCAT ACCAAAGTAC TATTATGTGC CAGCTGATTT TGTAGAGTAT 
GAAAAAAGAA ACCCTGGTAG TCAGAAGCGA TTCCCTAGCA ACTGTGGCCG TGATGGAAAA 
CTGTTTCTTT GGGGACAAGC CCTTTATGTC ATTGCAAAAC TCCTGGCTGA TGAATTAATT 
AGTCCTAAAG ACATTGATCC TGTTCAGCGT TTTGTCCCTC TACAGAATCA ACGAAATGTG 
AGCATGAGGT TTTCCAATCA GGGTCCACTG GAAAATGATT TGGTTGTTTA TGTAGCACTC 
ATAGCAGAAA GCCAACGCCT TCAAGTTTTC CTCAACACAT ATGGTATTCA GACTCAAACT 
CCTCAACAGG TGGAACCCAT TCAGATCTGG GCCCAGCAGG AGCTGGTGAA AGCTTATTTC 
CATCTGGGTA TCAATGAAAA ATTAGGACTC TGCGGAAGGC CAGATAGACC TATTGGCTGC 
CTTGGTACAT CAAAGATTTA TCGCATTCTA GGAAAGACTG TGGTTTGTTA CCCTATCATC 
TTTGACCTTA GTGATTTCTA CATGTCCCAA GATGTTTTGC TGCTGATAGA TGACATAAAG 
AATGCACTCC AGTTTATTAA GCAATACTGG AAAATGCATG GACGTCCACT TTTCCTTGTT 
CTCATCCGGG AAGACAATAT AAGAGGTAGC CGATTCAATC CCATATTAGA TATGCTGGCA 
GCATTTAAGA AAGGAATGGT TGGAGGAGTC AAAGTTCATG TTGATCGTCT ACAGACACTA 
ATATCTGGAG CTGTGGTTGA ACAACTTGAC TTCTTACGAA TCAGTGAAAT AGAAGAGCTT 
CCAGAATTTA AGAGTTTTGA GGAATTAGAA GTTCCCAAAC ATTCAAAAGT CAAACGACAA 
AGCAGCACCT CCAATGCTCC CGAACAGGAA CAGAAGCCAG ATGTTACCGT GACTGAATGG 
AGAAACAAAC CCACCCATGA AATTCTTCAA AAACTGAATG ACTGTAGTTG TCTGGCTAGC 
CAAGCCATCC TACTGGGTAT ACTGCTCAAA AGAGAAGGCC CCAACTTCAT CACAAAGGAC 
GGTACTGTTT CTGATCACAT TGAGAGAGTC TATAGAAGAG CTGGCAGCAA AAAACTTTGG 
TCGGTGGTGC GCCATGCAGC AAGTCTTTTA AGTAAACTAG TGGACAGCCT GGCCCCATCC 
ATTACTAATG TTTTAGTGCA GGGCAAACAG GTAACTCTGG GTGCCTTTGG GCATGAAGAA 
GAAGTTATCT CTAATCCTTT GTCTCCGGGA GTGATTAAGA ACATCATCTA TTACAAGTGT 
AATACCCATG ATGAGAGGGA GGCAGTAATT CAGCAAGAAC TGGTCATCCA CATTGGCTGG 
ATCATCTCCA ATAACCCAGA ATTATTCAGT GGCATGCTGA AAATACGAAT TGGGTGGATC 
ATCCATGCTA TGGAGTATGA ACTACAGATC CGTGGTGGAA ACAAGCCAGC CAGAGACTTA 
TATCAGCTGT CACCCAGTGA AGTAAAACAG CTTCTATTGG ATATTCTCCA GCCTCAACAG 
AATGGAAGAT GTTGGCTGAA CAGGCGGCAG ATCGATGGCT CTTTGAATAG AACCCCCCCT 
GGGTTCTATG ACAGAGTATG GCAGATCCTG GAACGCACAC CCAATGGAAT CATTGTAGCC 
GGGAGGCATT TGCCCCAGCA ACCAACCCTG TCAGATATGA CCATGTATGA GATGAATTTC 
TCTCTCCTTG TTGAAGACAT GTTGGGAAAT ATTGACCAGC CAAAATACAG ACAGATTGTC 
GTGGAGTTAC TAATGGTCGT ATCCATTGTA CTGGAAAGAA ACCCTGAGCT AGAATTTCAA 
GACAAAGTAG ATCTTGACAA ACTGGTGAAA GAAGCATTTC ATGAATTTCA AAAAGATGAG 
AGTCGACTAA AGGAAATTGA AAAACAAGAT GACATGACTT CCTTTTACAA CACTCCCCCT 
CTGGGAAAAA GAGGAACATG CAGCTATTTG ACAAAGGTCG TGATGAATCT GCTGCTGGAA 
GGAGAAGTCA AGCCAAGCAA TGATGACCCG TGTCTGGTTA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	PHKB
Entrez Gene ID	102391900
Full Name	phosphorylase kinase regulatory subunit beta
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

PHKB cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

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