rapgef6 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following rapgef6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rapgef6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa22425 NM_001045655.1
Latest version!
Xenopus tropicalis Rap guanine nucleotide exchange factor 6 (rapgef6), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OXa43161 XM_018092138.2
Latest version!
Xenopus tropicalis Rap guanine nucleotide exchange factor 6 (rapgef6), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa43159 XM_018092136.2
Latest version!
Xenopus tropicalis Rap guanine nucleotide exchange factor 6 (rapgef6), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa43160 XM_018092137.2
Latest version!
Xenopus tropicalis Rap guanine nucleotide exchange factor 6 (rapgef6), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa43153 NM_001374789.1
Latest version!
Xenopus tropicalis Rap guanine nucleotide exchange factor 6 (rapgef6), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa43153 XM_018092130.1
Latest version!
Xenopus tropicalis Rap guanine nucleotide exchange factor 6 (rapgef6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OXa43154 XM_018092131.2
Latest version!
Xenopus tropicalis Rap guanine nucleotide exchange factor 6 (rapgef6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa43155 XM_018092132.2
Latest version!
Xenopus tropicalis Rap guanine nucleotide exchange factor 6 (rapgef6), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa43156 XM_018092133.2
Latest version!
Xenopus tropicalis Rap guanine nucleotide exchange factor 6 (rapgef6), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa43157 XM_018092134.2
Latest version!
Xenopus tropicalis Rap guanine nucleotide exchange factor 6 (rapgef6), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa43158 XM_018092135.1
Latest version!
Xenopus tropicalis Rap guanine nucleotide exchange factor 6 (rapgef6), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OXa43162 XM_018092139.2
Latest version!
Xenopus tropicalis Rap guanine nucleotide exchange factor 6 (rapgef6), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
View Old Accession Versions >>
OXa43155 XM_018092132.1 Xenopus tropicalis Rap guanine nucleotide exchange factor 6 (rapgef6), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OXa43156 XM_018092133.1 Xenopus tropicalis Rap guanine nucleotide exchange factor 6 (rapgef6), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OXa43161 XM_018092138.1 Xenopus tropicalis Rap guanine nucleotide exchange factor 6 (rapgef6), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OXa43159 XM_018092136.1 Xenopus tropicalis Rap guanine nucleotide exchange factor 6 (rapgef6), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OXa43157 XM_018092134.1 Xenopus tropicalis Rap guanine nucleotide exchange factor 6 (rapgef6), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OXa43160 XM_018092137.1 Xenopus tropicalis Rap guanine nucleotide exchange factor 6 (rapgef6), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OXa43154 XM_018092131.1 Xenopus tropicalis Rap guanine nucleotide exchange factor 6 (rapgef6), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OXa43162 XM_018092139.1 Xenopus tropicalis Rap guanine nucleotide exchange factor 6 (rapgef6), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa22425
    Clone ID Related Accession (Same CDS sequence) NM_001045655.1
    Accession Version NM_001045655.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2271bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-10-27
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 6
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR942459.2. ##Evidence-Data-START## Transcript exon combination :: CR942459.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1025688, SAMEA1696418 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCTGCCA GCATTGAACC CAGGTTTAAA GAAGCCTTGA GGAAAAAACC CAAGGAAAGG 
    ACTCAGGAGG ATCTGAAAAC TGTTTATTCA TATATGCATA GAATGGATAT ACTCTCACAT
    CTCAGAGAAC ATCAATTAAG ATTGATGTCT GCAACAGCAC GATATGAAAG ATACAGTGCA
    AATCAGGTTC TATTCTGCTC AGAAAGTTTG GCTAGATGTT GGTACATTCT TCTTTCTGGT
    TCAGTTCTGG TAAAAAACTC CATGTTTTTG CCTCCATGCA GTTTTGGCAA GCAGTCAGGT
    GGCAAAAGGG GTTGTGATTG CATTGTACTG GAACCTTCTG AAATGATTGT GGTGGAAAAT
    ACAGGAGAAA ACAATGATGG CATTCTGCAT AGGGAAACTT CTATTAGACA ATCCCGGCGT
    CGGTATCGAA AAATCAACCA GCATGGAGAA CGCCAAACAA TTACAGATAA TGGGGATGCT
    GCTAGTTATG TCTTGCTCCC CACAGACCTC TCCAAGATAC ACCTCACCGA CAGTCCCCAC
    CCACATGTTA CACATGTGCC ATCCAGCCAA TCAGGATGCA GCATTGCAAG CGACTCTGGA
    AGCAGCAGCT TATCAGATAT CTACCAGGCA ACAGAAAGTG AGATGGGGGA TGTTGACTTG
    ACAGGCCTGC CAGAGGCAGC TGTTGATTCT GAAGATGAAG AGGAAGATGA AGATATTGAG
    AGAGCTTCAG ACACAATGCT GACCCGTGAC ATTGTGAGGG AATGTCTTGA GAAAGAACCC
    ACAGACAGAA CAGATGATGA CATAGAACAG CTACTGGAAT TCATGCATCA GCTTCCAGCC
    TTTGTCAATA TGACCATGTC TGTGAGACGA AAGCTTTGCT CGGTCATGAT TTTTGAAGTT
    GTGGAACAGG CTGGCGCAAT CATCATTGAA GATGGGCAAG AACTGGACTC TTGGTATGTC
    ATTCTTAATG GGATTGTAGA AATCAGCCAT TCAGATGGCA GGATGGAAAA CCTTTGCATG
    GGAAATAGCT TTGGCATAAA CCCATCTTTA GAAACACAGT ACATGGATGG AGTAGTAAAA
    ACCAAAGCTG ATGATTGCCA GTTTGTGTGC ATAGCACAAC AGGACTACTT TCGGATATTA
    AACCATGTGG AAAAAAATAC CCACAAAGTC AAGGAAAAGG GGGAGATTGT TATGGTTAAT
    GAACACCGAG AACTGGATCG AAGTGGCACT CGAAAGGGAC ATATAGTAAT TCAGGGGACT
    CCAGAAAGAC TCATCTTGCA CCTAATAGAA GAGCACTCTG TTGTTGACCC AACATACATA
    GAGGATTTTC TATTAACATA CAGGACATTC CTTTCAAGTC CATTAGAGGT TGGGGACAAG
    CTTTTGGAGT GGTTTAAAGA TGACAGTCGT CGTGACAAGG TAACACGAGT TGTCCTTCTT
    TGGGTTAACA ACCACTTTAA TGATTTTGAA GGAGACCCTG CCATGACAAG GTTTTTGGAA
    GAATTTGAAC GGAATCTTGA AAATGCGAAA ATGAAGGGTC ATCTGCGACT GCTAAACATA
    GCCTGTGCTG CAAAGGCTAA ACTGAGACAG GTTACATTGC TTAAGCCTTC CCGTGAATCG
    CCTTTTTATT TCAACGTCGT TGGGGGAAGC GACAAAGGTT TCGGCATGTT TATTGAAAGT
    GTTGAGCCTG GAAGCAAAGC AACAGAAGCT GGATTAAAAC GCGGAGATCA GATTATGGAA
    GTCAATGGCC AAAATTTTGA GACTATCGTT TACGCCAAAG CCCTGGAGAT CTTGAAAAAC
    AATACACATC TTTCCATCAC TGTCAAGACC AACATTTTTG TGTTTAAAGA GCTTCTTAAT
    CGAATCGGGC AGGAGAAAAA AAATGGAGTC CCACACATCC CCAAAATTGC AGAGAAGAAG
    AACAATAGAT ATTCCATCCC TGATCTCCCC AATGATGTAG AACAGATGTT TCCACGGGAT
    CGCGCAAGCA AGAAAATGAA AGCCAATACG GTGTCTGGAG GGCGGAATCG CATCCGGAAG
    ATTTTGGACA AAACACGTTT TAGTATATTA CCACCTAAGC CATTCAGTGA TGGCCATGTT
    ATCCAGTCCC AGGATGACAG TATTGTTGGC ACCAGGCAGT GCAGGCACAG TGTGGCTATA
    ATGCCCATTC CGGGCACGCT CTCTTCAAGC AGTCCAGACT TGCTTCAAGC TACAACAAAC
    ATATTGGATT TCTCCAATCC TACTGGTAAA ACTGCATGTG CAAAATTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001039120.1
    CDS387..2657
    Translation

    Target ORF information:

    RefSeq Version NM_001045655.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 6 (rapgef6), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001045655.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCTGCCA GCATTGAACC CAGGTTTAAA GAAGCCTTGA GGAAAAAACC CAAGGAAAGG 
    ACTCAGGAGG ATCTGAAAAC TGTTTATTCA TATATGCATA GAATGGATAT ACTCTCACAT
    CTCAGAGAAC ATCAATTAAG ATTGATGTCT GCAACAGCAC GATATGAAAG ATACAGTGCA
    AATCAGGTTC TATTCTGCTC AGAAAGTTTG GCTAGATGTT GGTACATTCT TCTTTCTGGT
    TCAGTTCTGG TAAAAAACTC CATGTTTTTG CCTCCATGCA GTTTTGGCAA GCAGTCAGGT
    GGCAAAAGGG GTTGTGATTG CATTGTACTG GAACCTTCTG AAATGATTGT GGTGGAAAAT
    ACAGGAGAAA ACAATGATGG CATTCTGCAT AGGGAAACTT CTATTAGACA ATCCCGGCGT
    CGGTATCGAA AAATCAACCA GCATGGAGAA CGCCAAACAA TTACAGATAA TGGGGATGCT
    GCTAGTTATG TCTTGCTCCC CACAGACCTC TCCAAGATAC ACCTCACCGA CAGTCCCCAC
    CCACATGTTA CACATGTGCC ATCCAGCCAA TCAGGATGCA GCATTGCAAG CGACTCTGGA
    AGCAGCAGCT TATCAGATAT CTACCAGGCA ACAGAAAGTG AGATGGGGGA TGTTGACTTG
    ACAGGCCTGC CAGAGGCAGC TGTTGATTCT GAAGATGAAG AGGAAGATGA AGATATTGAG
    AGAGCTTCAG ACACAATGCT GACCCGTGAC ATTGTGAGGG AATGTCTTGA GAAAGAACCC
    ACAGACAGAA CAGATGATGA CATAGAACAG CTACTGGAAT TCATGCATCA GCTTCCAGCC
    TTTGTCAATA TGACCATGTC TGTGAGACGA AAGCTTTGCT CGGTCATGAT TTTTGAAGTT
    GTGGAACAGG CTGGCGCAAT CATCATTGAA GATGGGCAAG AACTGGACTC TTGGTATGTC
    ATTCTTAATG GGATTGTAGA AATCAGCCAT TCAGATGGCA GGATGGAAAA CCTTTGCATG
    GGAAATAGCT TTGGCATAAA CCCATCTTTA GAAACACAGT ACATGGATGG AGTAGTAAAA
    ACCAAAGCTG ATGATTGCCA GTTTGTGTGC ATAGCACAAC AGGACTACTT TCGGATATTA
    AACCATGTGG AAAAAAATAC CCACAAAGTC AAGGAAAAGG GGGAGATTGT TATGGTTAAT
    GAACACCGAG AACTGGATCG AAGTGGCACT CGAAAGGGAC ATATAGTAAT TCAGGGGACT
    CCAGAAAGAC TCATCTTGCA CCTAATAGAA GAGCACTCTG TTGTTGACCC AACATACATA
    GAGGATTTTC TATTAACATA CAGGACATTC CTTTCAAGTC CATTAGAGGT TGGGGACAAG
    CTTTTGGAGT GGTTTAAAGA TGACAGTCGT CGTGACAAGG TAACACGAGT TGTCCTTCTT
    TGGGTTAACA ACCACTTTAA TGATTTTGAA GGAGACCCTG CCATGACAAG GTTTTTGGAA
    GAATTTGAAC GGAATCTTGA AAATGCGAAA ATGAAGGGTC ATCTGCGACT GCTAAACATA
    GCCTGTGCTG CAAAGGCTAA ACTGAGACAG GTTACATTGC TTAAGCCTTC CCGTGAATCG
    CCTTTTTATT TCAACGTCGT TGGGGGAAGC GACAAAGGTT TCGGCATGTT TATTGAAAGT
    GTTGAGCCTG GAAGCAAAGC AACAGAAGCT GGATTAAAAC GCGGAGATCA GATTATGGAA
    GTCAATGGCC AAAATTTTGA GACTATCGTT TACGCCAAAG CCCTGGAGAT CTTGAAAAAC
    AATACACATC TTTCCATCAC TGTCAAGACC AACATTTTTG TGTTTAAAGA GCTTCTTAAT
    CGAATCGGGC AGGAGAAAAA AAATGGAGTC CCACACATCC CCAAAATTGC AGAGAAGAAG
    AACAATAGAT ATTCCATCCC TGATCTCCCC AATGATGTAG AACAGATGTT TCCACGGGAT
    CGCGCAAGCA AGAAAATGAA AGCCAATACG GTGTCTGGAG GGCGGAATCG CATCCGGAAG
    ATTTTGGACA AAACACGTTT TAGTATATTA CCACCTAAGC CATTCAGTGA TGGCCATGTT
    ATCCAGTCCC AGGATGACAG TATTGTTGGC ACCAGGCAGT GCAGGCACAG TGTGGCTATA
    ATGCCCATTC CGGGCACGCT CTCTTCAAGC AGTCCAGACT TGCTTCAAGC TACAACAAAC
    ATATTGGATT TCTCCAATCC TACTGGTAAA ACTGCATGTG CAAAATTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa43161
    Clone ID Related Accession (Same CDS sequence) XM_018092138.1 , XM_018092138.2
    Accession Version XM_018092138.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2490bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 6 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTCTGCCA GCATTGAACC CAGGTTTAAA GAAGCCTTGA GGAAAAAACC CAAGGAAAGG 
    ACTCAGGAGG ATCTGAAAAC TGTTTATTCA TATATGCATA GAATGGATAT ACTCTCACAT
    CTCAGAGAAC ATCAATTAAG ATTGATGTCT GCAACAGCAC GATATGAAAG ATACAGTGCA
    AATCAGGTTC TATTCTGCTC AGAAAGTTTG GCTAGATGTT GGTACATTCT TCTTTCTGGT
    TCAGTTCTGG TAAAAAACTC CATGTTTTTG CCTCCATGCA GTTTTGGCAA GCAGTCAGGT
    GGCAAAAGGG GTTGTGATTG CATTGTACTG GAACCTTCTG AAATGATTGT GGTGGAAAAT
    ACAGGAGAAA ACAATGATGG CATTCTGCAT AGGGAAACTT CTATTAGACA ATCCCGGCGT
    CGGTATCGAA AAATCAACCA GCATGGAGAA CGCCAAACAA TTACAGATAA TGGGGATGCT
    GCTAGTTATG TCTTGCTCCC CACAGACCTC TCCAAGATAC ACCTCACCGA CAGTCCCCAC
    CCACATGTTA CACATGTGCC ATCCAGCCAA TCAGGATGCA GCATTGCAAG CGACTCTGGA
    AGCAGCAGCT TATCAGATAT CTACCAGGCA ACAGAAAGTG AGATGGGGGA TGTTGACTTG
    ACAGGCCTGC CAGAGGCAGC TGTTGATTCT GAAGATGAAG AGGAAGATGA AGATATTGAG
    AGAGCTTCAG ACACAATGCT GACCCGTGAC ATTGTGAGGG AATGTCTTGA GAAAGAACCC
    ACAGACAGAA CAGATGATGA CATAGAACAG CTACTGGAAT TCATGCATCA GCTTCCAGCC
    TTTGTCAATA TGACCATGTC TGTGAGACGA AAGCTTTGCT CGGTCATGAT TTTTGAAGTT
    GTGGAACAGG CTGGCGCAAT CATCATTGAA GATGGGCAAG AACTGGACTC TTGGTATGTC
    ATTCTTAATG GGATTGTAGA AATCAGCCAT TCAGATGGCA GGATGGAAAA CCTTTGCATG
    GGAAATAGCT TTGGCATAAA CCCATCTTTA GAAACACAGT ACATGGATGG AGTAGTAAAA
    ACCAAAGCTG ATGATTGCCA GTTTGTGTGC ATAGCACAAC AGGACTACTT TCGGATATTA
    AACCATGTGG AAAAAAATAC CCACAAAGTC AAGGAAAAGG GGGAGATTGT TATGGTTAAT
    GAACACCGAG AACTGGATCG AAGTGGCACT CGAAAGGGAC ATATAGTAAT TCAGGGGACT
    CCAGAAAGAC TCATCTTGCA CCTAATAGAA GAGCACTCTG TTGTTGACCC AACATACATA
    GAGGATTTTC TATTAACATA CAGGACATTC CTTTCAAGTC CATTAGAGGT TGGGGACAAG
    CTTTTGGAGT GGTTTAAAGA TGACAGTCGT CGTGACAAGG TAACACGAGT TGTCCTTCTT
    TGGGTTAACA ACCACTTTAA TGATTTTGAA GGAGACCCTG CCATGACAAG GTTTTTGGAA
    GAATTTGAAC GGAATCTTGA AAATGCGAAA ATGAAGGGTC ATCTGCGACT GCTAAACATA
    GCCTGTGCTG CAAAGGCTAA ACTGAGACAG GTTACATTGC TTAAGCCTTC CCGTGAATCG
    CCTTTTTATT TCAACGTCGT TGGGGGAAGC GACAAAGGTT TCGGCATGTT TATTGAAAGT
    GTTGAGCCTG GAAGCAAAGC AACAGAAGCT GGATTAAAAC GCGGAGATCA GATTATGGAA
    GTCAATGGCC AAAATTTTGA GACTATCGTT TACGCCAAAG CCCTGGAGAT CTTGAAAAAC
    AATACACATC TTTCCATCAC TGTCAAGACC AACATTTTTG TGTTTAAAGA GCTTCTTAAT
    CGAATCGGGC AGGAGAAAAA AAATGGAGTC CCACACATCC CCAAAATTGC AGAGAAGAAG
    AACAATAGAT ATTCCATCCC TGATCTCCCC AATGATGTAG AACAGATGTT TCCACGGGAT
    CGCGCAAGCA AGAAAATGAA AGCCAATACG GTGTCTGGAG GGCGGAATCG CATCCGGAAG
    ATTTTGGACA AAACACGTTT TAGTATATTA CCACCTAAGC CATTCAGTGA TGGCCATGTT
    ATCCAGTCCC AGGATGACAG TATTGTTGGC ACCAGGCAGT GCAGGCACAG TGTGGCTATA
    ATGCCCATTC CGGGCACGCT CTCTTCAAGC AGTCCAGACT TGCTTCAAGC TACAACAAAC
    ATATTGGATT TCTCCAATCC TACTGATATT CCAGACCAAG TTATACGAAT ATTTAAAGCA
    GATCAGCAGT GCTGCTATAT TATCATCGGC AAAGACACTA CTGCAAAGGA GGTGGTGATA
    CAAGCACTTC ATGAATTCAG CCTTACTGGG TCAACAGACA CATACTCTCT CTGTGAGGTC
    TCGGTCAGTC CAGAAGGTGT CATTAAACAA AGACGGCTTC CGGATCAGCT TTCTAAACTG
    GCAGACAGAA TCCAGCTGAA TGGAAGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947627.1
    CDS1365..3854
    Translation

    Target ORF information:

    RefSeq Version XM_018092138.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 6 (rapgef6), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092138.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTCTGCCA GCATTGAACC CAGGTTTAAA GAAGCCTTGA GGAAAAAACC CAAGGAAAGG 
    ACTCAGGAGG ATCTGAAAAC TGTTTATTCA TATATGCATA GAATGGATAT ACTCTCACAT
    CTCAGAGAAC ATCAATTAAG ATTGATGTCT GCAACAGCAC GATATGAAAG ATACAGTGCA
    AATCAGGTTC TATTCTGCTC AGAAAGTTTG GCTAGATGTT GGTACATTCT TCTTTCTGGT
    TCAGTTCTGG TAAAAAACTC CATGTTTTTG CCTCCATGCA GTTTTGGCAA GCAGTCAGGT
    GGCAAAAGGG GTTGTGATTG CATTGTACTG GAACCTTCTG AAATGATTGT GGTGGAAAAT
    ACAGGAGAAA ACAATGATGG CATTCTGCAT AGGGAAACTT CTATTAGACA ATCCCGGCGT
    CGGTATCGAA AAATCAACCA GCATGGAGAA CGCCAAACAA TTACAGATAA TGGGGATGCT
    GCTAGTTATG TCTTGCTCCC CACAGACCTC TCCAAGATAC ACCTCACCGA CAGTCCCCAC
    CCACATGTTA CACATGTGCC ATCCAGCCAA TCAGGATGCA GCATTGCAAG CGACTCTGGA
    AGCAGCAGCT TATCAGATAT CTACCAGGCA ACAGAAAGTG AGATGGGGGA TGTTGACTTG
    ACAGGCCTGC CAGAGGCAGC TGTTGATTCT GAAGATGAAG AGGAAGATGA AGATATTGAG
    AGAGCTTCAG ACACAATGCT GACCCGTGAC ATTGTGAGGG AATGTCTTGA GAAAGAACCC
    ACAGACAGAA CAGATGATGA CATAGAACAG CTACTGGAAT TCATGCATCA GCTTCCAGCC
    TTTGTCAATA TGACCATGTC TGTGAGACGA AAGCTTTGCT CGGTCATGAT TTTTGAAGTT
    GTGGAACAGG CTGGCGCAAT CATCATTGAA GATGGGCAAG AACTGGACTC TTGGTATGTC
    ATTCTTAATG GGATTGTAGA AATCAGCCAT TCAGATGGCA GGATGGAAAA CCTTTGCATG
    GGAAATAGCT TTGGCATAAA CCCATCTTTA GAAACACAGT ACATGGATGG AGTAGTAAAA
    ACCAAAGCTG ATGATTGCCA GTTTGTGTGC ATAGCACAAC AGGACTACTT TCGGATATTA
    AACCATGTGG AAAAAAATAC CCACAAAGTC AAGGAAAAGG GGGAGATTGT TATGGTTAAT
    GAACACCGAG AACTGGATCG AAGTGGCACT CGAAAGGGAC ATATAGTAAT TCAGGGGACT
    CCAGAAAGAC TCATCTTGCA CCTAATAGAA GAGCACTCTG TTGTTGACCC AACATACATA
    GAGGATTTTC TATTAACATA CAGGACATTC CTTTCAAGTC CATTAGAGGT TGGGGACAAG
    CTTTTGGAGT GGTTTAAAGA TGACAGTCGT CGTGACAAGG TAACACGAGT TGTCCTTCTT
    TGGGTTAACA ACCACTTTAA TGATTTTGAA GGAGACCCTG CCATGACAAG GTTTTTGGAA
    GAATTTGAAC GGAATCTTGA AAATGCGAAA ATGAAGGGTC ATCTGCGACT GCTAAACATA
    GCCTGTGCTG CAAAGGCTAA ACTGAGACAG GTTACATTGC TTAAGCCTTC CCGTGAATCG
    CCTTTTTATT TCAACGTCGT TGGGGGAAGC GACAAAGGTT TCGGCATGTT TATTGAAAGT
    GTTGAGCCTG GAAGCAAAGC AACAGAAGCT GGATTAAAAC GCGGAGATCA GATTATGGAA
    GTCAATGGCC AAAATTTTGA GACTATCGTT TACGCCAAAG CCCTGGAGAT CTTGAAAAAC
    AATACACATC TTTCCATCAC TGTCAAGACC AACATTTTTG TGTTTAAAGA GCTTCTTAAT
    CGAATCGGGC AGGAGAAAAA AAATGGAGTC CCACACATCC CCAAAATTGC AGAGAAGAAG
    AACAATAGAT ATTCCATCCC TGATCTCCCC AATGATGTAG AACAGATGTT TCCACGGGAT
    CGCGCAAGCA AGAAAATGAA AGCCAATACG GTGTCTGGAG GGCGGAATCG CATCCGGAAG
    ATTTTGGACA AAACACGTTT TAGTATATTA CCACCTAAGC CATTCAGTGA TGGCCATGTT
    ATCCAGTCCC AGGATGACAG TATTGTTGGC ACCAGGCAGT GCAGGCACAG TGTGGCTATA
    ATGCCCATTC CGGGCACGCT CTCTTCAAGC AGTCCAGACT TGCTTCAAGC TACAACAAAC
    ATATTGGATT TCTCCAATCC TACTGATATT CCAGACCAAG TTATACGAAT ATTTAAAGCA
    GATCAGCAGT GCTGCTATAT TATCATCGGC AAAGACACTA CTGCAAAGGA GGTGGTGATA
    CAAGCACTTC ATGAATTCAG CCTTACTGGG TCAACAGACA CATACTCTCT CTGTGAGGTC
    TCGGTCAGTC CAGAAGGTGT CATTAAACAA AGACGGCTTC CGGATCAGCT TTCTAAACTG
    GCAGACAGAA TCCAGCTGAA TGGAAGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa43161
    Clone ID Related Accession (Same CDS sequence) XM_018092138.1 , XM_018092138.2
    Accession Version XM_018092138.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2490bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 6 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018092138.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTCTGCCA GCATTGAACC CAGGTTTAAA GAAGCCTTGA GGAAAAAACC CAAGGAAAGG 
    ACTCAGGAGG ATCTGAAAAC TGTTTATTCA TATATGCATA GAATGGATAT ACTCTCACAT
    CTCAGAGAAC ATCAATTAAG ATTGATGTCT GCAACAGCAC GATATGAAAG ATACAGTGCA
    AATCAGGTTC TATTCTGCTC AGAAAGTTTG GCTAGATGTT GGTACATTCT TCTTTCTGGT
    TCAGTTCTGG TAAAAAACTC CATGTTTTTG CCTCCATGCA GTTTTGGCAA GCAGTCAGGT
    GGCAAAAGGG GTTGTGATTG CATTGTACTG GAACCTTCTG AAATGATTGT GGTGGAAAAT
    ACAGGAGAAA ACAATGATGG CATTCTGCAT AGGGAAACTT CTATTAGACA ATCCCGGCGT
    CGGTATCGAA AAATCAACCA GCATGGAGAA CGCCAAACAA TTACAGATAA TGGGGATGCT
    GCTAGTTATG TCTTGCTCCC CACAGACCTC TCCAAGATAC ACCTCACCGA CAGTCCCCAC
    CCACATGTTA CACATGTGCC ATCCAGCCAA TCAGGATGCA GCATTGCAAG CGACTCTGGA
    AGCAGCAGCT TATCAGATAT CTACCAGGCA ACAGAAAGTG AGATGGGGGA TGTTGACTTG
    ACAGGCCTGC CAGAGGCAGC TGTTGATTCT GAAGATGAAG AGGAAGATGA AGATATTGAG
    AGAGCTTCAG ACACAATGCT GACCCGTGAC ATTGTGAGGG AATGTCTTGA GAAAGAACCC
    ACAGACAGAA CAGATGATGA CATAGAACAG CTACTGGAAT TCATGCATCA GCTTCCAGCC
    TTTGTCAATA TGACCATGTC TGTGAGACGA AAGCTTTGCT CGGTCATGAT TTTTGAAGTT
    GTGGAACAGG CTGGCGCAAT CATCATTGAA GATGGGCAAG AACTGGACTC TTGGTATGTC
    ATTCTTAATG GGATTGTAGA AATCAGCCAT TCAGATGGCA GGATGGAAAA CCTTTGCATG
    GGAAATAGCT TTGGCATAAA CCCATCTTTA GAAACACAGT ACATGGATGG AGTAGTAAAA
    ACCAAAGCTG ATGATTGCCA GTTTGTGTGC ATAGCACAAC AGGACTACTT TCGGATATTA
    AACCATGTGG AAAAAAATAC CCACAAAGTC AAGGAAAAGG GGGAGATTGT TATGGTTAAT
    GAACACCGAG AACTGGATCG AAGTGGCACT CGAAAGGGAC ATATAGTAAT TCAGGGGACT
    CCAGAAAGAC TCATCTTGCA CCTAATAGAA GAGCACTCTG TTGTTGACCC AACATACATA
    GAGGATTTTC TATTAACATA CAGGACATTC CTTTCAAGTC CATTAGAGGT TGGGGACAAG
    CTTTTGGAGT GGTTTAAAGA TGACAGTCGT CGTGACAAGG TAACACGAGT TGTCCTTCTT
    TGGGTTAACA ACCACTTTAA TGATTTTGAA GGAGACCCTG CCATGACAAG GTTTTTGGAA
    GAATTTGAAC GGAATCTTGA AAATGCGAAA ATGAAGGGTC ATCTGCGACT GCTAAACATA
    GCCTGTGCTG CAAAGGCTAA ACTGAGACAG GTTACATTGC TTAAGCCTTC CCGTGAATCG
    CCTTTTTATT TCAACGTCGT TGGGGGAAGC GACAAAGGTT TCGGCATGTT TATTGAAAGT
    GTTGAGCCTG GAAGCAAAGC AACAGAAGCT GGATTAAAAC GCGGAGATCA GATTATGGAA
    GTCAATGGCC AAAATTTTGA GACTATCGTT TACGCCAAAG CCCTGGAGAT CTTGAAAAAC
    AATACACATC TTTCCATCAC TGTCAAGACC AACATTTTTG TGTTTAAAGA GCTTCTTAAT
    CGAATCGGGC AGGAGAAAAA AAATGGAGTC CCACACATCC CCAAAATTGC AGAGAAGAAG
    AACAATAGAT ATTCCATCCC TGATCTCCCC AATGATGTAG AACAGATGTT TCCACGGGAT
    CGCGCAAGCA AGAAAATGAA AGCCAATACG GTGTCTGGAG GGCGGAATCG CATCCGGAAG
    ATTTTGGACA AAACACGTTT TAGTATATTA CCACCTAAGC CATTCAGTGA TGGCCATGTT
    ATCCAGTCCC AGGATGACAG TATTGTTGGC ACCAGGCAGT GCAGGCACAG TGTGGCTATA
    ATGCCCATTC CGGGCACGCT CTCTTCAAGC AGTCCAGACT TGCTTCAAGC TACAACAAAC
    ATATTGGATT TCTCCAATCC TACTGATATT CCAGACCAAG TTATACGAAT ATTTAAAGCA
    GATCAGCAGT GCTGCTATAT TATCATCGGC AAAGACACTA CTGCAAAGGA GGTGGTGATA
    CAAGCACTTC ATGAATTCAG CCTTACTGGG TCAACAGACA CATACTCTCT CTGTGAGGTC
    TCGGTCAGTC CAGAAGGTGT CATTAAACAA AGACGGCTTC CGGATCAGCT TTCTAAACTG
    GCAGACAGAA TCCAGCTGAA TGGAAGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947627.1
    CDS489..2978
    Translation

    Target ORF information:

    RefSeq Version XM_018092138.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 6 (rapgef6), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092138.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTCTGCCA GCATTGAACC CAGGTTTAAA GAAGCCTTGA GGAAAAAACC CAAGGAAAGG 
    ACTCAGGAGG ATCTGAAAAC TGTTTATTCA TATATGCATA GAATGGATAT ACTCTCACAT
    CTCAGAGAAC ATCAATTAAG ATTGATGTCT GCAACAGCAC GATATGAAAG ATACAGTGCA
    AATCAGGTTC TATTCTGCTC AGAAAGTTTG GCTAGATGTT GGTACATTCT TCTTTCTGGT
    TCAGTTCTGG TAAAAAACTC CATGTTTTTG CCTCCATGCA GTTTTGGCAA GCAGTCAGGT
    GGCAAAAGGG GTTGTGATTG CATTGTACTG GAACCTTCTG AAATGATTGT GGTGGAAAAT
    ACAGGAGAAA ACAATGATGG CATTCTGCAT AGGGAAACTT CTATTAGACA ATCCCGGCGT
    CGGTATCGAA AAATCAACCA GCATGGAGAA CGCCAAACAA TTACAGATAA TGGGGATGCT
    GCTAGTTATG TCTTGCTCCC CACAGACCTC TCCAAGATAC ACCTCACCGA CAGTCCCCAC
    CCACATGTTA CACATGTGCC ATCCAGCCAA TCAGGATGCA GCATTGCAAG CGACTCTGGA
    AGCAGCAGCT TATCAGATAT CTACCAGGCA ACAGAAAGTG AGATGGGGGA TGTTGACTTG
    ACAGGCCTGC CAGAGGCAGC TGTTGATTCT GAAGATGAAG AGGAAGATGA AGATATTGAG
    AGAGCTTCAG ACACAATGCT GACCCGTGAC ATTGTGAGGG AATGTCTTGA GAAAGAACCC
    ACAGACAGAA CAGATGATGA CATAGAACAG CTACTGGAAT TCATGCATCA GCTTCCAGCC
    TTTGTCAATA TGACCATGTC TGTGAGACGA AAGCTTTGCT CGGTCATGAT TTTTGAAGTT
    GTGGAACAGG CTGGCGCAAT CATCATTGAA GATGGGCAAG AACTGGACTC TTGGTATGTC
    ATTCTTAATG GGATTGTAGA AATCAGCCAT TCAGATGGCA GGATGGAAAA CCTTTGCATG
    GGAAATAGCT TTGGCATAAA CCCATCTTTA GAAACACAGT ACATGGATGG AGTAGTAAAA
    ACCAAAGCTG ATGATTGCCA GTTTGTGTGC ATAGCACAAC AGGACTACTT TCGGATATTA
    AACCATGTGG AAAAAAATAC CCACAAAGTC AAGGAAAAGG GGGAGATTGT TATGGTTAAT
    GAACACCGAG AACTGGATCG AAGTGGCACT CGAAAGGGAC ATATAGTAAT TCAGGGGACT
    CCAGAAAGAC TCATCTTGCA CCTAATAGAA GAGCACTCTG TTGTTGACCC AACATACATA
    GAGGATTTTC TATTAACATA CAGGACATTC CTTTCAAGTC CATTAGAGGT TGGGGACAAG
    CTTTTGGAGT GGTTTAAAGA TGACAGTCGT CGTGACAAGG TAACACGAGT TGTCCTTCTT
    TGGGTTAACA ACCACTTTAA TGATTTTGAA GGAGACCCTG CCATGACAAG GTTTTTGGAA
    GAATTTGAAC GGAATCTTGA AAATGCGAAA ATGAAGGGTC ATCTGCGACT GCTAAACATA
    GCCTGTGCTG CAAAGGCTAA ACTGAGACAG GTTACATTGC TTAAGCCTTC CCGTGAATCG
    CCTTTTTATT TCAACGTCGT TGGGGGAAGC GACAAAGGTT TCGGCATGTT TATTGAAAGT
    GTTGAGCCTG GAAGCAAAGC AACAGAAGCT GGATTAAAAC GCGGAGATCA GATTATGGAA
    GTCAATGGCC AAAATTTTGA GACTATCGTT TACGCCAAAG CCCTGGAGAT CTTGAAAAAC
    AATACACATC TTTCCATCAC TGTCAAGACC AACATTTTTG TGTTTAAAGA GCTTCTTAAT
    CGAATCGGGC AGGAGAAAAA AAATGGAGTC CCACACATCC CCAAAATTGC AGAGAAGAAG
    AACAATAGAT ATTCCATCCC TGATCTCCCC AATGATGTAG AACAGATGTT TCCACGGGAT
    CGCGCAAGCA AGAAAATGAA AGCCAATACG GTGTCTGGAG GGCGGAATCG CATCCGGAAG
    ATTTTGGACA AAACACGTTT TAGTATATTA CCACCTAAGC CATTCAGTGA TGGCCATGTT
    ATCCAGTCCC AGGATGACAG TATTGTTGGC ACCAGGCAGT GCAGGCACAG TGTGGCTATA
    ATGCCCATTC CGGGCACGCT CTCTTCAAGC AGTCCAGACT TGCTTCAAGC TACAACAAAC
    ATATTGGATT TCTCCAATCC TACTGATATT CCAGACCAAG TTATACGAAT ATTTAAAGCA
    GATCAGCAGT GCTGCTATAT TATCATCGGC AAAGACACTA CTGCAAAGGA GGTGGTGATA
    CAAGCACTTC ATGAATTCAG CCTTACTGGG TCAACAGACA CATACTCTCT CTGTGAGGTC
    TCGGTCAGTC CAGAAGGTGT CATTAAACAA AGACGGCTTC CGGATCAGCT TTCTAAACTG
    GCAGACAGAA TCCAGCTGAA TGGAAGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa43159
    Clone ID Related Accession (Same CDS sequence) XM_018092136.2 , XM_018092136.1
    Accession Version XM_018092136.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4494bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 6 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018092136.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCATTCC TTGTAAGATG TTATGCCAAC TGTCTGCAGC CATGGGCTTC GAAACTCCCC 
    ACAGACCTCT CCAAGATACA CCTCACCGAC AGTCCCCACC CACATGTTAC ACATGTGCCA
    TCCAGCCAAT CAGGATGCAG CATTGCAAGC GACTCTGGAA GCAGCAGCTT ATCAGATATC
    TACCAGGCAA CAGAAAGTGA GATGGGGGAT GTTGACTTGA CAGGCCTGCC AGAGGCAGCT
    GTTGATTCTG AAGATGAAGA GGAAGATGAA GATATTGAGA GAGCTTCAGA CACAATGCTG
    ACCCGTGACA TTGTGAGGGA ATGTCTTGAG AAAGAACCCA CAGACAGAAC AGATGATGAC
    ATAGAACAGC TACTGGAATT CATGCATCAG CTTCCAGCCT TTGTCAATAT GACCATGTCT
    GTGAGACGAA AGCTTTGCTC GGTCATGATT TTTGAAGTTG TGGAACAGGC TGGCGCAATC
    ATCATTGAAG ATGGGCAAGA ACTGGACTCT TGGTATGTCA TTCTTAATGG GATTGTAGAA
    ATCAGCCATT CAGATGGCAG GATGGAAAAC CTTTGCATGG GAAATAGCTT TGGCATAAAC
    CCATCTTTAG AAACACAGTA CATGGATGGA GTAGTAAAAA CCAAAGCTGA TGATTGCCAG
    TTTGTGTGCA TAGCACAACA GGACTACTTT CGGATATTAA ACCATGTGGA AAAAAATACC
    CACAAAGTCA AGGAAAAGGG GGAGATTGTT ATGGTTAATG AACACCGAGA ACTGGATCGA
    AGTGGCACTC GAAAGGGACA TATAGTAATT CAGGGGACTC CAGAAAGACT CATCTTGCAC
    CTAATAGAAG AGCACTCTGT TGTTGACCCA ACATACATAG AGGATTTTCT ATTAACATAC
    AGGACATTCC TTTCAAGTCC ATTAGAGGTT GGGGACAAGC TTTTGGAGTG GTTTAAAGAT
    GACAGTCGTC GTGACAAGGT AACACGAGTT GTCCTTCTTT GGGTTAACAA CCACTTTAAT
    GATTTTGAAG GAGACCCTGC CATGACAAGG TTTTTGGAAG AATTTGAACG GAATCTTGAA
    AATGCGAAAA TGAAGGGTCA TCTGCGACTG CTAAACATAG CCTGTGCTGC AAAGGCTAAA
    CTGAGACAGG TTACATTGCT TAAGCCTTCC CGTGAATCGC CTTTTTATTT CAACGTCGTT
    GGGGGAAGCG ACAAAGGTTT CGGCATGTTT ATTGAAAGTG TTGAGCCTGG AAGCAAAGCA
    ACAGAAGCTG GATTAAAACG CGGAGATCAG ATTATGGAAG TCAATGGCCA AAATTTTGAG
    ACTATCGTTT ACGCCAAAGC CCTGGAGATC TTGAAAAACA ATACACATCT TTCCATCACT
    GTCAAGACCA ACATTTTTGT GTTTAAAGAG CTTCTTAATC GAATCGGGCA GGAGAAAAAA
    AATGGAGTCC CACACATCCC CAAAATTGCA GAGAAGAAGA ACAATAGATA TTCCATCCCT
    GATCTCCCCA ATGATGTAGA ACAGATGTTT CCACGGGATC GCGCAAGCAA GAAAATGAAA
    GCCAATACGG TGTCTGGAGG GCGGAATCGC ATCCGGAAGA TTTTGGACAA AACACGTTTT
    AGTATATTAC CACCTAAGCC ATTCAGTGAT GGCCATGTTA TCCAGTCCCA GGATGACAGT
    ATTGTTGGCA CCAGGCAGTG CAGGCACAGT GTGGCTATAA TGCCCATTCC GGGCACGCTC
    TCTTCAAGCA GTCCAGACTT GCTTCAAGCT ACAACAAACA TATTGGATTT CTCCAATCCT
    ACTGCTTTTG GATTTTACTA TATTCCAGAC CAAGTTATAC GAATATTTAA AGCAGATCAG
    CAGTGCTGCT ATATTATCAT CGGCAAAGAC ACTACTGCAA AGGAGGTGGT GATACAAGCA
    CTTCATGAAT TCAGCCTTAC TGGGTCAACA GACACATACT CTCTCTGTGA GGTCTCGGTC
    AGTCCAGAAG GTGTCATTAA ACAAAGACGG CTTCCGGATC AGCTTTCTAA ACTGGCAGAC
    AGAATCCAGC TGAATGGAAG GTATTATTTG AAGAACAACA TGGAGACCGA AACGTTGTGT
    TCAGATGAAG ATGCTCAGGA GCTTCTGCGT GAAAGCCTCA TCTCTCTCCT TCAGCTTAGC
    ACTATCGAGG TGGCAACACA GCTTTCTATG CGGGACTTTG AACTCTTCCG CAATATTGAG
    CCCACAGAAT ACATAGATGA CCTTTTCAAG ATAGATTCCA AGACAAGTGC TGCTCATCTG
    AAACAGTTTG AGGAAGTGAT CAACCAAGAG ACATTTTGGG TGGCCACGGA AATTCTAAGA
    GAACCAAACC ATTTGAAGAG AATGAAAATT GTGAAGCATT TCATAAAGAC TGCCCTCCAT
    TGCAGAGAAT GCAGAAATTT TAACTCCATG TTTGCTATAA TAAGTGGGCT CAATCTTGCA
    CCTGTGGCTC GGCTTCGAGG AACTTGGGAA AAGTTGCCCA GCAAATACGA GAAGCTGCTT
    CGAGACCTGC AAGATCTCTT CGACCCCTCA AGAAATATGG CGAAATACCG TAATATTCTA
    AGCAGTCAGA GCATGCAGCC CCCCATCATT CCTCTCTTCC CAGTCGTCAA GAAGGATATA
    ACATTTCTTC ATGAAGGAAA TGATTCAAAA GTTGATGGGT TAGTAAATTT TGAGAAGCTG
    CGAATGATTG CAAAGGAGAT TCGACATGTT ATTCGCATGA CTTCTGCTAA TATGGATCCA
    GCAGTTATGT TTAGGCAAAG GAAGAAGAGA TGGAGAAGTT TAGGGTCCCT AAGCCAAGGC
    AGCACAAACT CTAACATGCT GGATGTTCAA GGAGGTGGTC ACAAGAAACG GGCTCGGCGA
    AGTTCATTCC TTAATGCTAA GAAGCTGTAT GAAGATGCAC AGATGTCAAG GAAGGTAAAA
    CAGTACATCT CCAACCTGAG TGTGGAAATC GATGAGGAGA AGATCCAGCT AATGTCACTG
    CAATGTGAAC CAGCCTACAG CACTTTGGCA AAGAATTTAA GTGAGAGAAG ATCTGCAAAA
    TCTGACTTGT CTCCTGCACC TTTGAGGCCA TCAGGCCAAA CTACCAAAGC TCACATCCAT
    CAGCAAAACA GAATGAGCCA AGTATTGCAA GTCCCTACAG TTAACTTATA TCCAATGCGA
    AAGAAAGCAC CAACCAAGGA ACATCTTCCA TGCACTTCAG GATCTTCACA GAAAATATCT
    GGTTTGACAG ATGAGGTTAC AGTTAAAGCA CAGTTAGAAG ACTCTGTTTC TCTTGTGTCA
    TCATTGCATT CCAGCCCAGC CGCATCCCCT CAAGGATCTC CACATAAAGT TGTTAACATT
    TCAAGATCAG AGAATTCCAG TCATATGAAC CTCTCTGGAT CCTCCTCATC ATTAACCAGT
    GATCCCAACC AGAAGATCTG CAGTCGGCGT GGCTATGGAA TAGGCTATGC CCATAACCCC
    TCGGCCAAAT CTGATAACTT CTCAGACTCA AGCAACAGTG AGATCTCGTC CCGATCAAGC
    CTCGTCAGCA ACTGCTCAGT AGACTCCATG TCGTTGACCC TGCAGGATGA AAAGTGTGTG
    CCTCATTCAC ATGCTGAGTC AATGGGACTA TCAGAAAGAA AAGACGTCCA TCCGTCCTCT
    GAGTATAACC AGCCCTGCAG TAGCTGGCTC CATGCAAGGC ACCCTGCAGT CCGGAATTTC
    ATAGCTTGCG CAAGCAGTGA GGACATAGCT CATGACCACA TATCGGTAGA GCCGGTAGAC
    AGTGGTCGGG GCAGTTGGAC ATCTTGTTCT AGCAGTTCAC ATGACAATTT CCAGACTTTG
    CAGAAGGGAC TGGACCGCTT AAGCACTTAC CGCCAATCAC ACATTGATTC CCCAATTGCT
    GAGACAGAGT CCGCAAACTG CAGCACAGAT GAAACATGTA GTGAAGACTG TACCAAAGGA
    AAAAGGCACT CAAACAACAG TTTAGAACTA AATGAATCTC GGCAAAGTTG GGCTTCATCT
    AGCTCAATGT CAGATACTTA CGAGACGAAC TATGGCACAA TCAAGCGGAG AGTATTGGAC
    ACAGAGGAAA AAACAGAGGC TTTGGGCCAA CAATTAGATG TCTTTTATAA AACAGTTACT
    TCCACTACTG AAAAAGGACT GATAGTGTAC TGTGTCACCT CATCCCAGAA GGACAGTAGG
    TATAGGGAGC CACCTCCCAC TCCTCCAGGC TACCAGGGGA TATCATTAGC CGACATAAAG
    GAAGGACAAC CCCACCAAGC GCACTTAAAA CCTCCAGATT ATAGTGTGGC GATACAGAGG
    TCAAAGATGA TACATAAAGA CCTCTCTAGG CTTTCATCAG CCTGTGTTGA ACCAGTCTCA
    TGTGTCTCAC AGAAGATGAT AACCCATTGG CATAACCGGC ATCAGCCTTA TCCTAATGTA
    CAGGCTGACA GAGATAGTAA AGAAGATGAA GATGAACAAA TGTCAGCAGT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947625.1
    CDS635..5128
    Translation

    Target ORF information:

    RefSeq Version XM_018092136.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 6 (rapgef6), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092136.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCATTCC TTGTAAGATG TTATGCCAAC TGTCTGCAGC CATGGGCTTC GAAACTCCCC 
    ACAGACCTCT CCAAGATACA CCTCACCGAC AGTCCCCACC CACATGTTAC ACATGTGCCA
    TCCAGCCAAT CAGGATGCAG CATTGCAAGC GACTCTGGAA GCAGCAGCTT ATCAGATATC
    TACCAGGCAA CAGAAAGTGA GATGGGGGAT GTTGACTTGA CAGGCCTGCC AGAGGCAGCT
    GTTGATTCTG AAGATGAAGA GGAAGATGAA GATATTGAGA GAGCTTCAGA CACAATGCTG
    ACCCGTGACA TTGTGAGGGA ATGTCTTGAG AAAGAACCCA CAGACAGAAC AGATGATGAC
    ATAGAACAGC TACTGGAATT CATGCATCAG CTTCCAGCCT TTGTCAATAT GACCATGTCT
    GTGAGACGAA AGCTTTGCTC GGTCATGATT TTTGAAGTTG TGGAACAGGC TGGCGCAATC
    ATCATTGAAG ATGGGCAAGA ACTGGACTCT TGGTATGTCA TTCTTAATGG GATTGTAGAA
    ATCAGCCATT CAGATGGCAG GATGGAAAAC CTTTGCATGG GAAATAGCTT TGGCATAAAC
    CCATCTTTAG AAACACAGTA CATGGATGGA GTAGTAAAAA CCAAAGCTGA TGATTGCCAG
    TTTGTGTGCA TAGCACAACA GGACTACTTT CGGATATTAA ACCATGTGGA AAAAAATACC
    CACAAAGTCA AGGAAAAGGG GGAGATTGTT ATGGTTAATG AACACCGAGA ACTGGATCGA
    AGTGGCACTC GAAAGGGACA TATAGTAATT CAGGGGACTC CAGAAAGACT CATCTTGCAC
    CTAATAGAAG AGCACTCTGT TGTTGACCCA ACATACATAG AGGATTTTCT ATTAACATAC
    AGGACATTCC TTTCAAGTCC ATTAGAGGTT GGGGACAAGC TTTTGGAGTG GTTTAAAGAT
    GACAGTCGTC GTGACAAGGT AACACGAGTT GTCCTTCTTT GGGTTAACAA CCACTTTAAT
    GATTTTGAAG GAGACCCTGC CATGACAAGG TTTTTGGAAG AATTTGAACG GAATCTTGAA
    AATGCGAAAA TGAAGGGTCA TCTGCGACTG CTAAACATAG CCTGTGCTGC AAAGGCTAAA
    CTGAGACAGG TTACATTGCT TAAGCCTTCC CGTGAATCGC CTTTTTATTT CAACGTCGTT
    GGGGGAAGCG ACAAAGGTTT CGGCATGTTT ATTGAAAGTG TTGAGCCTGG AAGCAAAGCA
    ACAGAAGCTG GATTAAAACG CGGAGATCAG ATTATGGAAG TCAATGGCCA AAATTTTGAG
    ACTATCGTTT ACGCCAAAGC CCTGGAGATC TTGAAAAACA ATACACATCT TTCCATCACT
    GTCAAGACCA ACATTTTTGT GTTTAAAGAG CTTCTTAATC GAATCGGGCA GGAGAAAAAA
    AATGGAGTCC CACACATCCC CAAAATTGCA GAGAAGAAGA ACAATAGATA TTCCATCCCT
    GATCTCCCCA ATGATGTAGA ACAGATGTTT CCACGGGATC GCGCAAGCAA GAAAATGAAA
    GCCAATACGG TGTCTGGAGG GCGGAATCGC ATCCGGAAGA TTTTGGACAA AACACGTTTT
    AGTATATTAC CACCTAAGCC ATTCAGTGAT GGCCATGTTA TCCAGTCCCA GGATGACAGT
    ATTGTTGGCA CCAGGCAGTG CAGGCACAGT GTGGCTATAA TGCCCATTCC GGGCACGCTC
    TCTTCAAGCA GTCCAGACTT GCTTCAAGCT ACAACAAACA TATTGGATTT CTCCAATCCT
    ACTGCTTTTG GATTTTACTA TATTCCAGAC CAAGTTATAC GAATATTTAA AGCAGATCAG
    CAGTGCTGCT ATATTATCAT CGGCAAAGAC ACTACTGCAA AGGAGGTGGT GATACAAGCA
    CTTCATGAAT TCAGCCTTAC TGGGTCAACA GACACATACT CTCTCTGTGA GGTCTCGGTC
    AGTCCAGAAG GTGTCATTAA ACAAAGACGG CTTCCGGATC AGCTTTCTAA ACTGGCAGAC
    AGAATCCAGC TGAATGGAAG GTATTATTTG AAGAACAACA TGGAGACCGA AACGTTGTGT
    TCAGATGAAG ATGCTCAGGA GCTTCTGCGT GAAAGCCTCA TCTCTCTCCT TCAGCTTAGC
    ACTATCGAGG TGGCAACACA GCTTTCTATG CGGGACTTTG AACTCTTCCG CAATATTGAG
    CCCACAGAAT ACATAGATGA CCTTTTCAAG ATAGATTCCA AGACAAGTGC TGCTCATCTG
    AAACAGTTTG AGGAAGTGAT CAACCAAGAG ACATTTTGGG TGGCCACGGA AATTCTAAGA
    GAACCAAACC ATTTGAAGAG AATGAAAATT GTGAAGCATT TCATAAAGAC TGCCCTCCAT
    TGCAGAGAAT GCAGAAATTT TAACTCCATG TTTGCTATAA TAAGTGGGCT CAATCTTGCA
    CCTGTGGCTC GGCTTCGAGG AACTTGGGAA AAGTTGCCCA GCAAATACGA GAAGCTGCTT
    CGAGACCTGC AAGATCTCTT CGACCCCTCA AGAAATATGG CGAAATACCG TAATATTCTA
    AGCAGTCAGA GCATGCAGCC CCCCATCATT CCTCTCTTCC CAGTCGTCAA GAAGGATATA
    ACATTTCTTC ATGAAGGAAA TGATTCAAAA GTTGATGGGT TAGTAAATTT TGAGAAGCTG
    CGAATGATTG CAAAGGAGAT TCGACATGTT ATTCGCATGA CTTCTGCTAA TATGGATCCA
    GCAGTTATGT TTAGGCAAAG GAAGAAGAGA TGGAGAAGTT TAGGGTCCCT AAGCCAAGGC
    AGCACAAACT CTAACATGCT GGATGTTCAA GGAGGTGGTC ACAAGAAACG GGCTCGGCGA
    AGTTCATTCC TTAATGCTAA GAAGCTGTAT GAAGATGCAC AGATGTCAAG GAAGGTAAAA
    CAGTACATCT CCAACCTGAG TGTGGAAATC GATGAGGAGA AGATCCAGCT AATGTCACTG
    CAATGTGAAC CAGCCTACAG CACTTTGGCA AAGAATTTAA GTGAGAGAAG ATCTGCAAAA
    TCTGACTTGT CTCCTGCACC TTTGAGGCCA TCAGGCCAAA CTACCAAAGC TCACATCCAT
    CAGCAAAACA GAATGAGCCA AGTATTGCAA GTCCCTACAG TTAACTTATA TCCAATGCGA
    AAGAAAGCAC CAACCAAGGA ACATCTTCCA TGCACTTCAG GATCTTCACA GAAAATATCT
    GGTTTGACAG ATGAGGTTAC AGTTAAAGCA CAGTTAGAAG ACTCTGTTTC TCTTGTGTCA
    TCATTGCATT CCAGCCCAGC CGCATCCCCT CAAGGATCTC CACATAAAGT TGTTAACATT
    TCAAGATCAG AGAATTCCAG TCATATGAAC CTCTCTGGAT CCTCCTCATC ATTAACCAGT
    GATCCCAACC AGAAGATCTG CAGTCGGCGT GGCTATGGAA TAGGCTATGC CCATAACCCC
    TCGGCCAAAT CTGATAACTT CTCAGACTCA AGCAACAGTG AGATCTCGTC CCGATCAAGC
    CTCGTCAGCA ACTGCTCAGT AGACTCCATG TCGTTGACCC TGCAGGATGA AAAGTGTGTG
    CCTCATTCAC ATGCTGAGTC AATGGGACTA TCAGAAAGAA AAGACGTCCA TCCGTCCTCT
    GAGTATAACC AGCCCTGCAG TAGCTGGCTC CATGCAAGGC ACCCTGCAGT CCGGAATTTC
    ATAGCTTGCG CAAGCAGTGA GGACATAGCT CATGACCACA TATCGGTAGA GCCGGTAGAC
    AGTGGTCGGG GCAGTTGGAC ATCTTGTTCT AGCAGTTCAC ATGACAATTT CCAGACTTTG
    CAGAAGGGAC TGGACCGCTT AAGCACTTAC CGCCAATCAC ACATTGATTC CCCAATTGCT
    GAGACAGAGT CCGCAAACTG CAGCACAGAT GAAACATGTA GTGAAGACTG TACCAAAGGA
    AAAAGGCACT CAAACAACAG TTTAGAACTA AATGAATCTC GGCAAAGTTG GGCTTCATCT
    AGCTCAATGT CAGATACTTA CGAGACGAAC TATGGCACAA TCAAGCGGAG AGTATTGGAC
    ACAGAGGAAA AAACAGAGGC TTTGGGCCAA CAATTAGATG TCTTTTATAA AACAGTTACT
    TCCACTACTG AAAAAGGACT GATAGTGTAC TGTGTCACCT CATCCCAGAA GGACAGTAGG
    TATAGGGAGC CACCTCCCAC TCCTCCAGGC TACCAGGGGA TATCATTAGC CGACATAAAG
    GAAGGACAAC CCCACCAAGC GCACTTAAAA CCTCCAGATT ATAGTGTGGC GATACAGAGG
    TCAAAGATGA TACATAAAGA CCTCTCTAGG CTTTCATCAG CCTGTGTTGA ACCAGTCTCA
    TGTGTCTCAC AGAAGATGAT AACCCATTGG CATAACCGGC ATCAGCCTTA TCCTAATGTA
    CAGGCTGACA GAGATAGTAA AGAAGATGAA GATGAACAAA TGTCAGCAGT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa43159
    Clone ID Related Accession (Same CDS sequence) XM_018092136.2 , XM_018092136.1
    Accession Version XM_018092136.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4494bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 6 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCATTCC TTGTAAGATG TTATGCCAAC TGTCTGCAGC CATGGGCTTC GAAACTCCCC 
    ACAGACCTCT CCAAGATACA CCTCACCGAC AGTCCCCACC CACATGTTAC ACATGTGCCA
    TCCAGCCAAT CAGGATGCAG CATTGCAAGC GACTCTGGAA GCAGCAGCTT ATCAGATATC
    TACCAGGCAA CAGAAAGTGA GATGGGGGAT GTTGACTTGA CAGGCCTGCC AGAGGCAGCT
    GTTGATTCTG AAGATGAAGA GGAAGATGAA GATATTGAGA GAGCTTCAGA CACAATGCTG
    ACCCGTGACA TTGTGAGGGA ATGTCTTGAG AAAGAACCCA CAGACAGAAC AGATGATGAC
    ATAGAACAGC TACTGGAATT CATGCATCAG CTTCCAGCCT TTGTCAATAT GACCATGTCT
    GTGAGACGAA AGCTTTGCTC GGTCATGATT TTTGAAGTTG TGGAACAGGC TGGCGCAATC
    ATCATTGAAG ATGGGCAAGA ACTGGACTCT TGGTATGTCA TTCTTAATGG GATTGTAGAA
    ATCAGCCATT CAGATGGCAG GATGGAAAAC CTTTGCATGG GAAATAGCTT TGGCATAAAC
    CCATCTTTAG AAACACAGTA CATGGATGGA GTAGTAAAAA CCAAAGCTGA TGATTGCCAG
    TTTGTGTGCA TAGCACAACA GGACTACTTT CGGATATTAA ACCATGTGGA AAAAAATACC
    CACAAAGTCA AGGAAAAGGG GGAGATTGTT ATGGTTAATG AACACCGAGA ACTGGATCGA
    AGTGGCACTC GAAAGGGACA TATAGTAATT CAGGGGACTC CAGAAAGACT CATCTTGCAC
    CTAATAGAAG AGCACTCTGT TGTTGACCCA ACATACATAG AGGATTTTCT ATTAACATAC
    AGGACATTCC TTTCAAGTCC ATTAGAGGTT GGGGACAAGC TTTTGGAGTG GTTTAAAGAT
    GACAGTCGTC GTGACAAGGT AACACGAGTT GTCCTTCTTT GGGTTAACAA CCACTTTAAT
    GATTTTGAAG GAGACCCTGC CATGACAAGG TTTTTGGAAG AATTTGAACG GAATCTTGAA
    AATGCGAAAA TGAAGGGTCA TCTGCGACTG CTAAACATAG CCTGTGCTGC AAAGGCTAAA
    CTGAGACAGG TTACATTGCT TAAGCCTTCC CGTGAATCGC CTTTTTATTT CAACGTCGTT
    GGGGGAAGCG ACAAAGGTTT CGGCATGTTT ATTGAAAGTG TTGAGCCTGG AAGCAAAGCA
    ACAGAAGCTG GATTAAAACG CGGAGATCAG ATTATGGAAG TCAATGGCCA AAATTTTGAG
    ACTATCGTTT ACGCCAAAGC CCTGGAGATC TTGAAAAACA ATACACATCT TTCCATCACT
    GTCAAGACCA ACATTTTTGT GTTTAAAGAG CTTCTTAATC GAATCGGGCA GGAGAAAAAA
    AATGGAGTCC CACACATCCC CAAAATTGCA GAGAAGAAGA ACAATAGATA TTCCATCCCT
    GATCTCCCCA ATGATGTAGA ACAGATGTTT CCACGGGATC GCGCAAGCAA GAAAATGAAA
    GCCAATACGG TGTCTGGAGG GCGGAATCGC ATCCGGAAGA TTTTGGACAA AACACGTTTT
    AGTATATTAC CACCTAAGCC ATTCAGTGAT GGCCATGTTA TCCAGTCCCA GGATGACAGT
    ATTGTTGGCA CCAGGCAGTG CAGGCACAGT GTGGCTATAA TGCCCATTCC GGGCACGCTC
    TCTTCAAGCA GTCCAGACTT GCTTCAAGCT ACAACAAACA TATTGGATTT CTCCAATCCT
    ACTGCTTTTG GATTTTACTA TATTCCAGAC CAAGTTATAC GAATATTTAA AGCAGATCAG
    CAGTGCTGCT ATATTATCAT CGGCAAAGAC ACTACTGCAA AGGAGGTGGT GATACAAGCA
    CTTCATGAAT TCAGCCTTAC TGGGTCAACA GACACATACT CTCTCTGTGA GGTCTCGGTC
    AGTCCAGAAG GTGTCATTAA ACAAAGACGG CTTCCGGATC AGCTTTCTAA ACTGGCAGAC
    AGAATCCAGC TGAATGGAAG GTATTATTTG AAGAACAACA TGGAGACCGA AACGTTGTGT
    TCAGATGAAG ATGCTCAGGA GCTTCTGCGT GAAAGCCTCA TCTCTCTCCT TCAGCTTAGC
    ACTATCGAGG TGGCAACACA GCTTTCTATG CGGGACTTTG AACTCTTCCG CAATATTGAG
    CCCACAGAAT ACATAGATGA CCTTTTCAAG ATAGATTCCA AGACAAGTGC TGCTCATCTG
    AAACAGTTTG AGGAAGTGAT CAACCAAGAG ACATTTTGGG TGGCCACGGA AATTCTAAGA
    GAACCAAACC ATTTGAAGAG AATGAAAATT GTGAAGCATT TCATAAAGAC TGCCCTCCAT
    TGCAGAGAAT GCAGAAATTT TAACTCCATG TTTGCTATAA TAAGTGGGCT CAATCTTGCA
    CCTGTGGCTC GGCTTCGAGG AACTTGGGAA AAGTTGCCCA GCAAATACGA GAAGCTGCTT
    CGAGACCTGC AAGATCTCTT CGACCCCTCA AGAAATATGG CGAAATACCG TAATATTCTA
    AGCAGTCAGA GCATGCAGCC CCCCATCATT CCTCTCTTCC CAGTCGTCAA GAAGGATATA
    ACATTTCTTC ATGAAGGAAA TGATTCAAAA GTTGATGGGT TAGTAAATTT TGAGAAGCTG
    CGAATGATTG CAAAGGAGAT TCGACATGTT ATTCGCATGA CTTCTGCTAA TATGGATCCA
    GCAGTTATGT TTAGGCAAAG GAAGAAGAGA TGGAGAAGTT TAGGGTCCCT AAGCCAAGGC
    AGCACAAACT CTAACATGCT GGATGTTCAA GGAGGTGGTC ACAAGAAACG GGCTCGGCGA
    AGTTCATTCC TTAATGCTAA GAAGCTGTAT GAAGATGCAC AGATGTCAAG GAAGGTAAAA
    CAGTACATCT CCAACCTGAG TGTGGAAATC GATGAGGAGA AGATCCAGCT AATGTCACTG
    CAATGTGAAC CAGCCTACAG CACTTTGGCA AAGAATTTAA GTGAGAGAAG ATCTGCAAAA
    TCTGACTTGT CTCCTGCACC TTTGAGGCCA TCAGGCCAAA CTACCAAAGC TCACATCCAT
    CAGCAAAACA GAATGAGCCA AGTATTGCAA GTCCCTACAG TTAACTTATA TCCAATGCGA
    AAGAAAGCAC CAACCAAGGA ACATCTTCCA TGCACTTCAG GATCTTCACA GAAAATATCT
    GGTTTGACAG ATGAGGTTAC AGTTAAAGCA CAGTTAGAAG ACTCTGTTTC TCTTGTGTCA
    TCATTGCATT CCAGCCCAGC CGCATCCCCT CAAGGATCTC CACATAAAGT TGTTAACATT
    TCAAGATCAG AGAATTCCAG TCATATGAAC CTCTCTGGAT CCTCCTCATC ATTAACCAGT
    GATCCCAACC AGAAGATCTG CAGTCGGCGT GGCTATGGAA TAGGCTATGC CCATAACCCC
    TCGGCCAAAT CTGATAACTT CTCAGACTCA AGCAACAGTG AGATCTCGTC CCGATCAAGC
    CTCGTCAGCA ACTGCTCAGT AGACTCCATG TCGTTGACCC TGCAGGATGA AAAGTGTGTG
    CCTCATTCAC ATGCTGAGTC AATGGGACTA TCAGAAAGAA AAGACGTCCA TCCGTCCTCT
    GAGTATAACC AGCCCTGCAG TAGCTGGCTC CATGCAAGGC ACCCTGCAGT CCGGAATTTC
    ATAGCTTGCG CAAGCAGTGA GGACATAGCT CATGACCACA TATCGGTAGA GCCGGTAGAC
    AGTGGTCGGG GCAGTTGGAC ATCTTGTTCT AGCAGTTCAC ATGACAATTT CCAGACTTTG
    CAGAAGGGAC TGGACCGCTT AAGCACTTAC CGCCAATCAC ACATTGATTC CCCAATTGCT
    GAGACAGAGT CCGCAAACTG CAGCACAGAT GAAACATGTA GTGAAGACTG TACCAAAGGA
    AAAAGGCACT CAAACAACAG TTTAGAACTA AATGAATCTC GGCAAAGTTG GGCTTCATCT
    AGCTCAATGT CAGATACTTA CGAGACGAAC TATGGCACAA TCAAGCGGAG AGTATTGGAC
    ACAGAGGAAA AAACAGAGGC TTTGGGCCAA CAATTAGATG TCTTTTATAA AACAGTTACT
    TCCACTACTG AAAAAGGACT GATAGTGTAC TGTGTCACCT CATCCCAGAA GGACAGTAGG
    TATAGGGAGC CACCTCCCAC TCCTCCAGGC TACCAGGGGA TATCATTAGC CGACATAAAG
    GAAGGACAAC CCCACCAAGC GCACTTAAAA CCTCCAGATT ATAGTGTGGC GATACAGAGG
    TCAAAGATGA TACATAAAGA CCTCTCTAGG CTTTCATCAG CCTGTGTTGA ACCAGTCTCA
    TGTGTCTCAC AGAAGATGAT AACCCATTGG CATAACCGGC ATCAGCCTTA TCCTAATGTA
    CAGGCTGACA GAGATAGTAA AGAAGATGAA GATGAACAAA TGTCAGCAGT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947625.1
    CDS1093..5586
    Translation

    Target ORF information:

    RefSeq Version XM_018092136.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 6 (rapgef6), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092136.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCATTCC TTGTAAGATG TTATGCCAAC TGTCTGCAGC CATGGGCTTC GAAACTCCCC 
    ACAGACCTCT CCAAGATACA CCTCACCGAC AGTCCCCACC CACATGTTAC ACATGTGCCA
    TCCAGCCAAT CAGGATGCAG CATTGCAAGC GACTCTGGAA GCAGCAGCTT ATCAGATATC
    TACCAGGCAA CAGAAAGTGA GATGGGGGAT GTTGACTTGA CAGGCCTGCC AGAGGCAGCT
    GTTGATTCTG AAGATGAAGA GGAAGATGAA GATATTGAGA GAGCTTCAGA CACAATGCTG
    ACCCGTGACA TTGTGAGGGA ATGTCTTGAG AAAGAACCCA CAGACAGAAC AGATGATGAC
    ATAGAACAGC TACTGGAATT CATGCATCAG CTTCCAGCCT TTGTCAATAT GACCATGTCT
    GTGAGACGAA AGCTTTGCTC GGTCATGATT TTTGAAGTTG TGGAACAGGC TGGCGCAATC
    ATCATTGAAG ATGGGCAAGA ACTGGACTCT TGGTATGTCA TTCTTAATGG GATTGTAGAA
    ATCAGCCATT CAGATGGCAG GATGGAAAAC CTTTGCATGG GAAATAGCTT TGGCATAAAC
    CCATCTTTAG AAACACAGTA CATGGATGGA GTAGTAAAAA CCAAAGCTGA TGATTGCCAG
    TTTGTGTGCA TAGCACAACA GGACTACTTT CGGATATTAA ACCATGTGGA AAAAAATACC
    CACAAAGTCA AGGAAAAGGG GGAGATTGTT ATGGTTAATG AACACCGAGA ACTGGATCGA
    AGTGGCACTC GAAAGGGACA TATAGTAATT CAGGGGACTC CAGAAAGACT CATCTTGCAC
    CTAATAGAAG AGCACTCTGT TGTTGACCCA ACATACATAG AGGATTTTCT ATTAACATAC
    AGGACATTCC TTTCAAGTCC ATTAGAGGTT GGGGACAAGC TTTTGGAGTG GTTTAAAGAT
    GACAGTCGTC GTGACAAGGT AACACGAGTT GTCCTTCTTT GGGTTAACAA CCACTTTAAT
    GATTTTGAAG GAGACCCTGC CATGACAAGG TTTTTGGAAG AATTTGAACG GAATCTTGAA
    AATGCGAAAA TGAAGGGTCA TCTGCGACTG CTAAACATAG CCTGTGCTGC AAAGGCTAAA
    CTGAGACAGG TTACATTGCT TAAGCCTTCC CGTGAATCGC CTTTTTATTT CAACGTCGTT
    GGGGGAAGCG ACAAAGGTTT CGGCATGTTT ATTGAAAGTG TTGAGCCTGG AAGCAAAGCA
    ACAGAAGCTG GATTAAAACG CGGAGATCAG ATTATGGAAG TCAATGGCCA AAATTTTGAG
    ACTATCGTTT ACGCCAAAGC CCTGGAGATC TTGAAAAACA ATACACATCT TTCCATCACT
    GTCAAGACCA ACATTTTTGT GTTTAAAGAG CTTCTTAATC GAATCGGGCA GGAGAAAAAA
    AATGGAGTCC CACACATCCC CAAAATTGCA GAGAAGAAGA ACAATAGATA TTCCATCCCT
    GATCTCCCCA ATGATGTAGA ACAGATGTTT CCACGGGATC GCGCAAGCAA GAAAATGAAA
    GCCAATACGG TGTCTGGAGG GCGGAATCGC ATCCGGAAGA TTTTGGACAA AACACGTTTT
    AGTATATTAC CACCTAAGCC ATTCAGTGAT GGCCATGTTA TCCAGTCCCA GGATGACAGT
    ATTGTTGGCA CCAGGCAGTG CAGGCACAGT GTGGCTATAA TGCCCATTCC GGGCACGCTC
    TCTTCAAGCA GTCCAGACTT GCTTCAAGCT ACAACAAACA TATTGGATTT CTCCAATCCT
    ACTGCTTTTG GATTTTACTA TATTCCAGAC CAAGTTATAC GAATATTTAA AGCAGATCAG
    CAGTGCTGCT ATATTATCAT CGGCAAAGAC ACTACTGCAA AGGAGGTGGT GATACAAGCA
    CTTCATGAAT TCAGCCTTAC TGGGTCAACA GACACATACT CTCTCTGTGA GGTCTCGGTC
    AGTCCAGAAG GTGTCATTAA ACAAAGACGG CTTCCGGATC AGCTTTCTAA ACTGGCAGAC
    AGAATCCAGC TGAATGGAAG GTATTATTTG AAGAACAACA TGGAGACCGA AACGTTGTGT
    TCAGATGAAG ATGCTCAGGA GCTTCTGCGT GAAAGCCTCA TCTCTCTCCT TCAGCTTAGC
    ACTATCGAGG TGGCAACACA GCTTTCTATG CGGGACTTTG AACTCTTCCG CAATATTGAG
    CCCACAGAAT ACATAGATGA CCTTTTCAAG ATAGATTCCA AGACAAGTGC TGCTCATCTG
    AAACAGTTTG AGGAAGTGAT CAACCAAGAG ACATTTTGGG TGGCCACGGA AATTCTAAGA
    GAACCAAACC ATTTGAAGAG AATGAAAATT GTGAAGCATT TCATAAAGAC TGCCCTCCAT
    TGCAGAGAAT GCAGAAATTT TAACTCCATG TTTGCTATAA TAAGTGGGCT CAATCTTGCA
    CCTGTGGCTC GGCTTCGAGG AACTTGGGAA AAGTTGCCCA GCAAATACGA GAAGCTGCTT
    CGAGACCTGC AAGATCTCTT CGACCCCTCA AGAAATATGG CGAAATACCG TAATATTCTA
    AGCAGTCAGA GCATGCAGCC CCCCATCATT CCTCTCTTCC CAGTCGTCAA GAAGGATATA
    ACATTTCTTC ATGAAGGAAA TGATTCAAAA GTTGATGGGT TAGTAAATTT TGAGAAGCTG
    CGAATGATTG CAAAGGAGAT TCGACATGTT ATTCGCATGA CTTCTGCTAA TATGGATCCA
    GCAGTTATGT TTAGGCAAAG GAAGAAGAGA TGGAGAAGTT TAGGGTCCCT AAGCCAAGGC
    AGCACAAACT CTAACATGCT GGATGTTCAA GGAGGTGGTC ACAAGAAACG GGCTCGGCGA
    AGTTCATTCC TTAATGCTAA GAAGCTGTAT GAAGATGCAC AGATGTCAAG GAAGGTAAAA
    CAGTACATCT CCAACCTGAG TGTGGAAATC GATGAGGAGA AGATCCAGCT AATGTCACTG
    CAATGTGAAC CAGCCTACAG CACTTTGGCA AAGAATTTAA GTGAGAGAAG ATCTGCAAAA
    TCTGACTTGT CTCCTGCACC TTTGAGGCCA TCAGGCCAAA CTACCAAAGC TCACATCCAT
    CAGCAAAACA GAATGAGCCA AGTATTGCAA GTCCCTACAG TTAACTTATA TCCAATGCGA
    AAGAAAGCAC CAACCAAGGA ACATCTTCCA TGCACTTCAG GATCTTCACA GAAAATATCT
    GGTTTGACAG ATGAGGTTAC AGTTAAAGCA CAGTTAGAAG ACTCTGTTTC TCTTGTGTCA
    TCATTGCATT CCAGCCCAGC CGCATCCCCT CAAGGATCTC CACATAAAGT TGTTAACATT
    TCAAGATCAG AGAATTCCAG TCATATGAAC CTCTCTGGAT CCTCCTCATC ATTAACCAGT
    GATCCCAACC AGAAGATCTG CAGTCGGCGT GGCTATGGAA TAGGCTATGC CCATAACCCC
    TCGGCCAAAT CTGATAACTT CTCAGACTCA AGCAACAGTG AGATCTCGTC CCGATCAAGC
    CTCGTCAGCA ACTGCTCAGT AGACTCCATG TCGTTGACCC TGCAGGATGA AAAGTGTGTG
    CCTCATTCAC ATGCTGAGTC AATGGGACTA TCAGAAAGAA AAGACGTCCA TCCGTCCTCT
    GAGTATAACC AGCCCTGCAG TAGCTGGCTC CATGCAAGGC ACCCTGCAGT CCGGAATTTC
    ATAGCTTGCG CAAGCAGTGA GGACATAGCT CATGACCACA TATCGGTAGA GCCGGTAGAC
    AGTGGTCGGG GCAGTTGGAC ATCTTGTTCT AGCAGTTCAC ATGACAATTT CCAGACTTTG
    CAGAAGGGAC TGGACCGCTT AAGCACTTAC CGCCAATCAC ACATTGATTC CCCAATTGCT
    GAGACAGAGT CCGCAAACTG CAGCACAGAT GAAACATGTA GTGAAGACTG TACCAAAGGA
    AAAAGGCACT CAAACAACAG TTTAGAACTA AATGAATCTC GGCAAAGTTG GGCTTCATCT
    AGCTCAATGT CAGATACTTA CGAGACGAAC TATGGCACAA TCAAGCGGAG AGTATTGGAC
    ACAGAGGAAA AAACAGAGGC TTTGGGCCAA CAATTAGATG TCTTTTATAA AACAGTTACT
    TCCACTACTG AAAAAGGACT GATAGTGTAC TGTGTCACCT CATCCCAGAA GGACAGTAGG
    TATAGGGAGC CACCTCCCAC TCCTCCAGGC TACCAGGGGA TATCATTAGC CGACATAAAG
    GAAGGACAAC CCCACCAAGC GCACTTAAAA CCTCCAGATT ATAGTGTGGC GATACAGAGG
    TCAAAGATGA TACATAAAGA CCTCTCTAGG CTTTCATCAG CCTGTGTTGA ACCAGTCTCA
    TGTGTCTCAC AGAAGATGAT AACCCATTGG CATAACCGGC ATCAGCCTTA TCCTAATGTA
    CAGGCTGACA GAGATAGTAA AGAAGATGAA GATGAACAAA TGTCAGCAGT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa43160
    Clone ID Related Accession (Same CDS sequence) XM_018092137.1 , XM_018092137.2
    Accession Version XM_018092137.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3948bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 6 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCTGCCA GCATTGAACC CAGGTTTAAA GAAGCCTTGA GGAAAAAACC CAAGGAAAGG 
    ACTCAGGAGG ATCTGAAAAC TGTTTATTCA TATATGCATA GAATGGATAT ACTCTCACAT
    CTCAGAGAAC ATCAATTAAG ATTGATGTCT GCAACAGCAC GATATGAAAG ATACAGTGCA
    AATCAGGTTC TATTCTGCTC AGAAAGTTTG GCTAGATGTT GGTACATTCT TCTTTCTGGT
    TCAGTTCTGG TAAAAAACTC CATGTTTTTG CCTCCATGCA GTTTTGGCAA GCAGTCAGGT
    GGCAAAAGGG GTTGTGATTG CATTGTACTG GAACCTTCTG AAATGATTGT GGTGGAAAAT
    ACAGGAGAAA ACAATGATGG CATTCTGCAT AGGGAAACTT CTATTAGACA ATCCCGGCGT
    CGGTATCGAA AAATCAACCA GCATGGAGAA CGCCAAACAA TTACAGATAA TGGGGATGCT
    GCTAGTTATG TCTTGCTCCC CACAGACCTC TCCAAGATAC ACCTCACCGA CAGTCCCCAC
    CCACATGTTA CACATGTGCC ATCCAGCCAA TCAGGATGCA GCATTGCAAG CGACTCTGGA
    AGCAGCAGCT TATCAGATAT CTACCAGGCA ACAGAAAGTG AGATGGGGGA TGTTGACTTG
    ACAGGCCTGC CAGAGGCAGC TGTTGATTCT GAAGATGAAG AGGAAGATGA AGATATTGAG
    AGAGCTTCAG ACACAATGCT GACCCGTGAC ATTGTGAGGG AATGTCTTGA GAAAGAACCC
    ACAGACAGAA CAGATGATGA CATAGAACAG CTACTGGAAT TCATGCATCA GCTTCCAGCC
    TTTGTCAATA TGACCATGTC TGTGAGACGA AAGCTTTGCT CGGTCATGAT TTTTGAAGTT
    GTGGAACAGG CTGGCGCAAT CATCATTGAA GATGGGCAAG AACTGGACTC TTGGTATGTC
    ATTCTTAATG GGATTGTAGA AATCAGCCAT TCAGATGGCA GGATGGAAAA CCTTTGCATG
    GGAAATAGCT TTGGCATAAA CCCATCTTTA GAAACACAGT ACATGGATGG AGTAGTAAAA
    ACCAAAGCTG ATGATTGCCA GTTTGTGTGC ATAGCACAAC AGGACTACTT TCGGATATTA
    AACCATGTGG AAAAAAATAC CCACAAAGTC AAGGAAAAGG GGGAGATTGT TATGGTTAAT
    GAACACCGAG AACTGGATCG AAGTGGCACT CGAAAGGGAC ATATAGTAAT TCAGGGGACT
    CCAGAAAGAC TCATCTTGCA CCTAATAGAA GAGCACTCTG TTGTTGACCC AACATACATA
    GAGGATTTTC TATTAACATA CAGGACATTC CTTTCAAGTC CATTAGAGGT TGGGGACAAG
    CTTTTGGAGT GGTTTAAAGA TGACAGTCGT CGTGACAAGG TAACACGAGT TGTCCTTCTT
    TGGGTTAACA ACCACTTTAA TGATTTTGAA GGAGACCCTG CCATGACAAG GTTTTTGGAA
    GAATTTGAAC GGAATCTTGA AAATGCGAAA ATGAAGGGTC ATCTGCGACT GCTAAACATA
    GCCTGTGCTG CAAAGGCTAA ACTGAGACAG GTTACATTGC TTAAGCCTTC CCGTGAATCG
    CCTTTTTATT TCAACGTCGT TGGGGGAAGC GACAAAGGTT TCGGCATGTT TATTGAAAGT
    GTTGAGCCTG GAAGCAAAGC AACAGAAGCT GGATTAAAAC GCGGAGATCA GATTATGGAA
    GTCAATGGCC AAAATTTTGA GACTATCGTT TACGCCAAAG CCCTGGAGAT CTTGAAAAAC
    AATACACATC TTTCCATCAC TGTCAAGACC AACATTTTTG TGTTTAAAGA GCTTCTTAAT
    CGAATCGGGC AGGAGAAAAA AAATGGAGTC CCACACATCC CCAAAATTGC AGAGAAGAAG
    AACAATAGAT ATTCCATCCC TGATCTCCCC AATGATGTAG AACAGATGTT TCCACGGGAT
    CGCGCAAGCA AGAAAATGAA AGCCAATACG GTGTCTGGAG GGCGGAATCG CATCCGGAAG
    ATTTTGGACA AAACACGTTT TAGTATATTA CCACCTAAGC CATTCAGTGA TGGCCATGTT
    ATCCAGTCCC AGGATGACAG TATTGTTGGC ACCAGGCAGT GCAGGCACAG TGTGGCTATA
    ATGCCCATTC CGGGCACGCT CTCTTCAAGC AGTCCAGACT TGCTTCAAGC TACAACAAAC
    ATATTGGATT TCTCCAATCC TACTGATATT CCAGACCAAG TTATACGAAT ATTTAAAGCA
    GATCAGCAGT GCTGCTATAT TATCATCGGC AAAGACACTA CTGCAAAGGA GGTGGTGATA
    CAAGCACTTC ATGAATTCAG CCTTACTGGG TCAACAGACA CATACTCTCT CTGTGAGGTC
    TCGGTCAGTC CAGAAGGTGT CATTAAACAA AGACGGCTTC CGGATCAGCT TTCTAAACTG
    GCAGACAGAA TCCAGCTGAA TGGAAGGTAT TATTTGAAGA ACAACATGGA GACCGAAACG
    TTGTGTTCAG ATGAAGATGC TCAGGAGCTT CTGCGTGAAA GCCTCATCTC TCTCCTTCAG
    CTTAGCACTA TCGAGGTGGC AACACAGCTT TCTATGCGGG ACTTTGAACT CTTCCGCAAT
    ATTGAGCCCA CAGAATACAT AGATGACCTT TTCAAGATAG ATTCCAAGAC AAGTGCTGCT
    CATCTGAAAC AGTTTGAGGA AGTGATCAAC CAAGAGACAT TTTGGGTGGC CACGGAAATT
    CTAAGAGAAC CAAACCATTT GAAGAGAATG AAAATTGTGA AGCATTTCAT AAAGACTGCC
    CTCCATTGCA GAGAATGCAG AAATTTTAAC TCCATGTTTG CTATAATAAG TGGGCTCAAT
    CTTGCACCTG TGGCTCGGCT TCGAGGAACT TGGGAAAAGT TGCCCAGCAA ATACGAGAAG
    CTGCTTCGAG ACCTGCAAGA TCTCTTCGAC CCCTCAAGAA ATATGGCGAA ATACCGTAAT
    ATTCTAAGCA GTCAGAGCAT GCAGCCCCCC ATCATTCCTC TCTTCCCAGT CGTCAAGAAG
    GATATAACAT TTCTTCATGA AGGAAATGAT TCAAAAGTTG ATGGGTTAGT AAATTTTGAG
    AAGCTGCGAA TGATTGCAAA GGAGATTCGA CATGTTATTC GCATGACTTC TGCTAATATG
    GATCCAGCAG TTATGTTTAG GCAAAGGTCC CTAAGCCAAG GCAGCACAAA CTCTAACATG
    CTGGATGTTC AAGGAGGTGG TCACAAGAAA CGGGCTCGGC GAAGTTCATT CCTTAATGCT
    AAGAAGCTGT ATGAAGATGC ACAGATGTCA AGGAAGGTAA AACAGTACAT CTCCAACCTG
    AGTGTGGAAA TCGATGAGGA GAAGATCCAG CTAATGTCAC TGCAATGTGA ACCAGCCTAC
    AGCACTTTGG CAAAGAATTT AAGTGAGAGA AGATCTGCAA AATCTGACTT GTCTCCTGCA
    CCTTTGAGGC CATCAGGCCA AACTACCAAA GCTCACATCC ATCAGCAAAA CAGAATGAGC
    CAAGTATTGC AAGTCCCTAC AGTTAACTTA TATCCAATGC GAAAGAAAGC ACCAACCAAG
    GAACATCTTC CATGCACTTC AGGATCTTCA CAGAAAATAT CTGGTTTGAC AGATGAGGTT
    ACAGTTAAAG CACAGTTAGA AGACTCTGTT TCTCTTGTGT CATCATTGCA TTCCAGCCCA
    GCCGCATCCC CTCAAGGATC TCCACATAAA GTTGTTAACA TTTCAAGATC AGAGAATTCC
    AGTCATATGA ACCTCTCTGG ATCCTCCTCA TCATTAACCA GTGATCCCAA CCAGAAGATC
    TGCAGTCGGC GTGGCTATGG AATAGATATG TTAAGAGAGA CAGCAAAACA GAGGAAAGTT
    GGGACCTCTA GTGAGGCCTC TGAGGGAGGA AAAAGCAGAG AGATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947626.1
    CDS1366..5313
    Translation

    Target ORF information:

    RefSeq Version XM_018092137.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 6 (rapgef6), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092137.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCTGCCA GCATTGAACC CAGGTTTAAA GAAGCCTTGA GGAAAAAACC CAAGGAAAGG 
    ACTCAGGAGG ATCTGAAAAC TGTTTATTCA TATATGCATA GAATGGATAT ACTCTCACAT
    CTCAGAGAAC ATCAATTAAG ATTGATGTCT GCAACAGCAC GATATGAAAG ATACAGTGCA
    AATCAGGTTC TATTCTGCTC AGAAAGTTTG GCTAGATGTT GGTACATTCT TCTTTCTGGT
    TCAGTTCTGG TAAAAAACTC CATGTTTTTG CCTCCATGCA GTTTTGGCAA GCAGTCAGGT
    GGCAAAAGGG GTTGTGATTG CATTGTACTG GAACCTTCTG AAATGATTGT GGTGGAAAAT
    ACAGGAGAAA ACAATGATGG CATTCTGCAT AGGGAAACTT CTATTAGACA ATCCCGGCGT
    CGGTATCGAA AAATCAACCA GCATGGAGAA CGCCAAACAA TTACAGATAA TGGGGATGCT
    GCTAGTTATG TCTTGCTCCC CACAGACCTC TCCAAGATAC ACCTCACCGA CAGTCCCCAC
    CCACATGTTA CACATGTGCC ATCCAGCCAA TCAGGATGCA GCATTGCAAG CGACTCTGGA
    AGCAGCAGCT TATCAGATAT CTACCAGGCA ACAGAAAGTG AGATGGGGGA TGTTGACTTG
    ACAGGCCTGC CAGAGGCAGC TGTTGATTCT GAAGATGAAG AGGAAGATGA AGATATTGAG
    AGAGCTTCAG ACACAATGCT GACCCGTGAC ATTGTGAGGG AATGTCTTGA GAAAGAACCC
    ACAGACAGAA CAGATGATGA CATAGAACAG CTACTGGAAT TCATGCATCA GCTTCCAGCC
    TTTGTCAATA TGACCATGTC TGTGAGACGA AAGCTTTGCT CGGTCATGAT TTTTGAAGTT
    GTGGAACAGG CTGGCGCAAT CATCATTGAA GATGGGCAAG AACTGGACTC TTGGTATGTC
    ATTCTTAATG GGATTGTAGA AATCAGCCAT TCAGATGGCA GGATGGAAAA CCTTTGCATG
    GGAAATAGCT TTGGCATAAA CCCATCTTTA GAAACACAGT ACATGGATGG AGTAGTAAAA
    ACCAAAGCTG ATGATTGCCA GTTTGTGTGC ATAGCACAAC AGGACTACTT TCGGATATTA
    AACCATGTGG AAAAAAATAC CCACAAAGTC AAGGAAAAGG GGGAGATTGT TATGGTTAAT
    GAACACCGAG AACTGGATCG AAGTGGCACT CGAAAGGGAC ATATAGTAAT TCAGGGGACT
    CCAGAAAGAC TCATCTTGCA CCTAATAGAA GAGCACTCTG TTGTTGACCC AACATACATA
    GAGGATTTTC TATTAACATA CAGGACATTC CTTTCAAGTC CATTAGAGGT TGGGGACAAG
    CTTTTGGAGT GGTTTAAAGA TGACAGTCGT CGTGACAAGG TAACACGAGT TGTCCTTCTT
    TGGGTTAACA ACCACTTTAA TGATTTTGAA GGAGACCCTG CCATGACAAG GTTTTTGGAA
    GAATTTGAAC GGAATCTTGA AAATGCGAAA ATGAAGGGTC ATCTGCGACT GCTAAACATA
    GCCTGTGCTG CAAAGGCTAA ACTGAGACAG GTTACATTGC TTAAGCCTTC CCGTGAATCG
    CCTTTTTATT TCAACGTCGT TGGGGGAAGC GACAAAGGTT TCGGCATGTT TATTGAAAGT
    GTTGAGCCTG GAAGCAAAGC AACAGAAGCT GGATTAAAAC GCGGAGATCA GATTATGGAA
    GTCAATGGCC AAAATTTTGA GACTATCGTT TACGCCAAAG CCCTGGAGAT CTTGAAAAAC
    AATACACATC TTTCCATCAC TGTCAAGACC AACATTTTTG TGTTTAAAGA GCTTCTTAAT
    CGAATCGGGC AGGAGAAAAA AAATGGAGTC CCACACATCC CCAAAATTGC AGAGAAGAAG
    AACAATAGAT ATTCCATCCC TGATCTCCCC AATGATGTAG AACAGATGTT TCCACGGGAT
    CGCGCAAGCA AGAAAATGAA AGCCAATACG GTGTCTGGAG GGCGGAATCG CATCCGGAAG
    ATTTTGGACA AAACACGTTT TAGTATATTA CCACCTAAGC CATTCAGTGA TGGCCATGTT
    ATCCAGTCCC AGGATGACAG TATTGTTGGC ACCAGGCAGT GCAGGCACAG TGTGGCTATA
    ATGCCCATTC CGGGCACGCT CTCTTCAAGC AGTCCAGACT TGCTTCAAGC TACAACAAAC
    ATATTGGATT TCTCCAATCC TACTGATATT CCAGACCAAG TTATACGAAT ATTTAAAGCA
    GATCAGCAGT GCTGCTATAT TATCATCGGC AAAGACACTA CTGCAAAGGA GGTGGTGATA
    CAAGCACTTC ATGAATTCAG CCTTACTGGG TCAACAGACA CATACTCTCT CTGTGAGGTC
    TCGGTCAGTC CAGAAGGTGT CATTAAACAA AGACGGCTTC CGGATCAGCT TTCTAAACTG
    GCAGACAGAA TCCAGCTGAA TGGAAGGTAT TATTTGAAGA ACAACATGGA GACCGAAACG
    TTGTGTTCAG ATGAAGATGC TCAGGAGCTT CTGCGTGAAA GCCTCATCTC TCTCCTTCAG
    CTTAGCACTA TCGAGGTGGC AACACAGCTT TCTATGCGGG ACTTTGAACT CTTCCGCAAT
    ATTGAGCCCA CAGAATACAT AGATGACCTT TTCAAGATAG ATTCCAAGAC AAGTGCTGCT
    CATCTGAAAC AGTTTGAGGA AGTGATCAAC CAAGAGACAT TTTGGGTGGC CACGGAAATT
    CTAAGAGAAC CAAACCATTT GAAGAGAATG AAAATTGTGA AGCATTTCAT AAAGACTGCC
    CTCCATTGCA GAGAATGCAG AAATTTTAAC TCCATGTTTG CTATAATAAG TGGGCTCAAT
    CTTGCACCTG TGGCTCGGCT TCGAGGAACT TGGGAAAAGT TGCCCAGCAA ATACGAGAAG
    CTGCTTCGAG ACCTGCAAGA TCTCTTCGAC CCCTCAAGAA ATATGGCGAA ATACCGTAAT
    ATTCTAAGCA GTCAGAGCAT GCAGCCCCCC ATCATTCCTC TCTTCCCAGT CGTCAAGAAG
    GATATAACAT TTCTTCATGA AGGAAATGAT TCAAAAGTTG ATGGGTTAGT AAATTTTGAG
    AAGCTGCGAA TGATTGCAAA GGAGATTCGA CATGTTATTC GCATGACTTC TGCTAATATG
    GATCCAGCAG TTATGTTTAG GCAAAGGTCC CTAAGCCAAG GCAGCACAAA CTCTAACATG
    CTGGATGTTC AAGGAGGTGG TCACAAGAAA CGGGCTCGGC GAAGTTCATT CCTTAATGCT
    AAGAAGCTGT ATGAAGATGC ACAGATGTCA AGGAAGGTAA AACAGTACAT CTCCAACCTG
    AGTGTGGAAA TCGATGAGGA GAAGATCCAG CTAATGTCAC TGCAATGTGA ACCAGCCTAC
    AGCACTTTGG CAAAGAATTT AAGTGAGAGA AGATCTGCAA AATCTGACTT GTCTCCTGCA
    CCTTTGAGGC CATCAGGCCA AACTACCAAA GCTCACATCC ATCAGCAAAA CAGAATGAGC
    CAAGTATTGC AAGTCCCTAC AGTTAACTTA TATCCAATGC GAAAGAAAGC ACCAACCAAG
    GAACATCTTC CATGCACTTC AGGATCTTCA CAGAAAATAT CTGGTTTGAC AGATGAGGTT
    ACAGTTAAAG CACAGTTAGA AGACTCTGTT TCTCTTGTGT CATCATTGCA TTCCAGCCCA
    GCCGCATCCC CTCAAGGATC TCCACATAAA GTTGTTAACA TTTCAAGATC AGAGAATTCC
    AGTCATATGA ACCTCTCTGG ATCCTCCTCA TCATTAACCA GTGATCCCAA CCAGAAGATC
    TGCAGTCGGC GTGGCTATGG AATAGATATG TTAAGAGAGA CAGCAAAACA GAGGAAAGTT
    GGGACCTCTA GTGAGGCCTC TGAGGGAGGA AAAAGCAGAG AGATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa43160
    Clone ID Related Accession (Same CDS sequence) XM_018092137.1 , XM_018092137.2
    Accession Version XM_018092137.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3948bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 6 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018092137.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCTGCCA GCATTGAACC CAGGTTTAAA GAAGCCTTGA GGAAAAAACC CAAGGAAAGG 
    ACTCAGGAGG ATCTGAAAAC TGTTTATTCA TATATGCATA GAATGGATAT ACTCTCACAT
    CTCAGAGAAC ATCAATTAAG ATTGATGTCT GCAACAGCAC GATATGAAAG ATACAGTGCA
    AATCAGGTTC TATTCTGCTC AGAAAGTTTG GCTAGATGTT GGTACATTCT TCTTTCTGGT
    TCAGTTCTGG TAAAAAACTC CATGTTTTTG CCTCCATGCA GTTTTGGCAA GCAGTCAGGT
    GGCAAAAGGG GTTGTGATTG CATTGTACTG GAACCTTCTG AAATGATTGT GGTGGAAAAT
    ACAGGAGAAA ACAATGATGG CATTCTGCAT AGGGAAACTT CTATTAGACA ATCCCGGCGT
    CGGTATCGAA AAATCAACCA GCATGGAGAA CGCCAAACAA TTACAGATAA TGGGGATGCT
    GCTAGTTATG TCTTGCTCCC CACAGACCTC TCCAAGATAC ACCTCACCGA CAGTCCCCAC
    CCACATGTTA CACATGTGCC ATCCAGCCAA TCAGGATGCA GCATTGCAAG CGACTCTGGA
    AGCAGCAGCT TATCAGATAT CTACCAGGCA ACAGAAAGTG AGATGGGGGA TGTTGACTTG
    ACAGGCCTGC CAGAGGCAGC TGTTGATTCT GAAGATGAAG AGGAAGATGA AGATATTGAG
    AGAGCTTCAG ACACAATGCT GACCCGTGAC ATTGTGAGGG AATGTCTTGA GAAAGAACCC
    ACAGACAGAA CAGATGATGA CATAGAACAG CTACTGGAAT TCATGCATCA GCTTCCAGCC
    TTTGTCAATA TGACCATGTC TGTGAGACGA AAGCTTTGCT CGGTCATGAT TTTTGAAGTT
    GTGGAACAGG CTGGCGCAAT CATCATTGAA GATGGGCAAG AACTGGACTC TTGGTATGTC
    ATTCTTAATG GGATTGTAGA AATCAGCCAT TCAGATGGCA GGATGGAAAA CCTTTGCATG
    GGAAATAGCT TTGGCATAAA CCCATCTTTA GAAACACAGT ACATGGATGG AGTAGTAAAA
    ACCAAAGCTG ATGATTGCCA GTTTGTGTGC ATAGCACAAC AGGACTACTT TCGGATATTA
    AACCATGTGG AAAAAAATAC CCACAAAGTC AAGGAAAAGG GGGAGATTGT TATGGTTAAT
    GAACACCGAG AACTGGATCG AAGTGGCACT CGAAAGGGAC ATATAGTAAT TCAGGGGACT
    CCAGAAAGAC TCATCTTGCA CCTAATAGAA GAGCACTCTG TTGTTGACCC AACATACATA
    GAGGATTTTC TATTAACATA CAGGACATTC CTTTCAAGTC CATTAGAGGT TGGGGACAAG
    CTTTTGGAGT GGTTTAAAGA TGACAGTCGT CGTGACAAGG TAACACGAGT TGTCCTTCTT
    TGGGTTAACA ACCACTTTAA TGATTTTGAA GGAGACCCTG CCATGACAAG GTTTTTGGAA
    GAATTTGAAC GGAATCTTGA AAATGCGAAA ATGAAGGGTC ATCTGCGACT GCTAAACATA
    GCCTGTGCTG CAAAGGCTAA ACTGAGACAG GTTACATTGC TTAAGCCTTC CCGTGAATCG
    CCTTTTTATT TCAACGTCGT TGGGGGAAGC GACAAAGGTT TCGGCATGTT TATTGAAAGT
    GTTGAGCCTG GAAGCAAAGC AACAGAAGCT GGATTAAAAC GCGGAGATCA GATTATGGAA
    GTCAATGGCC AAAATTTTGA GACTATCGTT TACGCCAAAG CCCTGGAGAT CTTGAAAAAC
    AATACACATC TTTCCATCAC TGTCAAGACC AACATTTTTG TGTTTAAAGA GCTTCTTAAT
    CGAATCGGGC AGGAGAAAAA AAATGGAGTC CCACACATCC CCAAAATTGC AGAGAAGAAG
    AACAATAGAT ATTCCATCCC TGATCTCCCC AATGATGTAG AACAGATGTT TCCACGGGAT
    CGCGCAAGCA AGAAAATGAA AGCCAATACG GTGTCTGGAG GGCGGAATCG CATCCGGAAG
    ATTTTGGACA AAACACGTTT TAGTATATTA CCACCTAAGC CATTCAGTGA TGGCCATGTT
    ATCCAGTCCC AGGATGACAG TATTGTTGGC ACCAGGCAGT GCAGGCACAG TGTGGCTATA
    ATGCCCATTC CGGGCACGCT CTCTTCAAGC AGTCCAGACT TGCTTCAAGC TACAACAAAC
    ATATTGGATT TCTCCAATCC TACTGATATT CCAGACCAAG TTATACGAAT ATTTAAAGCA
    GATCAGCAGT GCTGCTATAT TATCATCGGC AAAGACACTA CTGCAAAGGA GGTGGTGATA
    CAAGCACTTC ATGAATTCAG CCTTACTGGG TCAACAGACA CATACTCTCT CTGTGAGGTC
    TCGGTCAGTC CAGAAGGTGT CATTAAACAA AGACGGCTTC CGGATCAGCT TTCTAAACTG
    GCAGACAGAA TCCAGCTGAA TGGAAGGTAT TATTTGAAGA ACAACATGGA GACCGAAACG
    TTGTGTTCAG ATGAAGATGC TCAGGAGCTT CTGCGTGAAA GCCTCATCTC TCTCCTTCAG
    CTTAGCACTA TCGAGGTGGC AACACAGCTT TCTATGCGGG ACTTTGAACT CTTCCGCAAT
    ATTGAGCCCA CAGAATACAT AGATGACCTT TTCAAGATAG ATTCCAAGAC AAGTGCTGCT
    CATCTGAAAC AGTTTGAGGA AGTGATCAAC CAAGAGACAT TTTGGGTGGC CACGGAAATT
    CTAAGAGAAC CAAACCATTT GAAGAGAATG AAAATTGTGA AGCATTTCAT AAAGACTGCC
    CTCCATTGCA GAGAATGCAG AAATTTTAAC TCCATGTTTG CTATAATAAG TGGGCTCAAT
    CTTGCACCTG TGGCTCGGCT TCGAGGAACT TGGGAAAAGT TGCCCAGCAA ATACGAGAAG
    CTGCTTCGAG ACCTGCAAGA TCTCTTCGAC CCCTCAAGAA ATATGGCGAA ATACCGTAAT
    ATTCTAAGCA GTCAGAGCAT GCAGCCCCCC ATCATTCCTC TCTTCCCAGT CGTCAAGAAG
    GATATAACAT TTCTTCATGA AGGAAATGAT TCAAAAGTTG ATGGGTTAGT AAATTTTGAG
    AAGCTGCGAA TGATTGCAAA GGAGATTCGA CATGTTATTC GCATGACTTC TGCTAATATG
    GATCCAGCAG TTATGTTTAG GCAAAGGTCC CTAAGCCAAG GCAGCACAAA CTCTAACATG
    CTGGATGTTC AAGGAGGTGG TCACAAGAAA CGGGCTCGGC GAAGTTCATT CCTTAATGCT
    AAGAAGCTGT ATGAAGATGC ACAGATGTCA AGGAAGGTAA AACAGTACAT CTCCAACCTG
    AGTGTGGAAA TCGATGAGGA GAAGATCCAG CTAATGTCAC TGCAATGTGA ACCAGCCTAC
    AGCACTTTGG CAAAGAATTT AAGTGAGAGA AGATCTGCAA AATCTGACTT GTCTCCTGCA
    CCTTTGAGGC CATCAGGCCA AACTACCAAA GCTCACATCC ATCAGCAAAA CAGAATGAGC
    CAAGTATTGC AAGTCCCTAC AGTTAACTTA TATCCAATGC GAAAGAAAGC ACCAACCAAG
    GAACATCTTC CATGCACTTC AGGATCTTCA CAGAAAATAT CTGGTTTGAC AGATGAGGTT
    ACAGTTAAAG CACAGTTAGA AGACTCTGTT TCTCTTGTGT CATCATTGCA TTCCAGCCCA
    GCCGCATCCC CTCAAGGATC TCCACATAAA GTTGTTAACA TTTCAAGATC AGAGAATTCC
    AGTCATATGA ACCTCTCTGG ATCCTCCTCA TCATTAACCA GTGATCCCAA CCAGAAGATC
    TGCAGTCGGC GTGGCTATGG AATAGATATG TTAAGAGAGA CAGCAAAACA GAGGAAAGTT
    GGGACCTCTA GTGAGGCCTC TGAGGGAGGA AAAAGCAGAG AGATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947626.1
    CDS491..4438
    Translation

    Target ORF information:

    RefSeq Version XM_018092137.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 6 (rapgef6), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092137.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCTGCCA GCATTGAACC CAGGTTTAAA GAAGCCTTGA GGAAAAAACC CAAGGAAAGG 
    ACTCAGGAGG ATCTGAAAAC TGTTTATTCA TATATGCATA GAATGGATAT ACTCTCACAT
    CTCAGAGAAC ATCAATTAAG ATTGATGTCT GCAACAGCAC GATATGAAAG ATACAGTGCA
    AATCAGGTTC TATTCTGCTC AGAAAGTTTG GCTAGATGTT GGTACATTCT TCTTTCTGGT
    TCAGTTCTGG TAAAAAACTC CATGTTTTTG CCTCCATGCA GTTTTGGCAA GCAGTCAGGT
    GGCAAAAGGG GTTGTGATTG CATTGTACTG GAACCTTCTG AAATGATTGT GGTGGAAAAT
    ACAGGAGAAA ACAATGATGG CATTCTGCAT AGGGAAACTT CTATTAGACA ATCCCGGCGT
    CGGTATCGAA AAATCAACCA GCATGGAGAA CGCCAAACAA TTACAGATAA TGGGGATGCT
    GCTAGTTATG TCTTGCTCCC CACAGACCTC TCCAAGATAC ACCTCACCGA CAGTCCCCAC
    CCACATGTTA CACATGTGCC ATCCAGCCAA TCAGGATGCA GCATTGCAAG CGACTCTGGA
    AGCAGCAGCT TATCAGATAT CTACCAGGCA ACAGAAAGTG AGATGGGGGA TGTTGACTTG
    ACAGGCCTGC CAGAGGCAGC TGTTGATTCT GAAGATGAAG AGGAAGATGA AGATATTGAG
    AGAGCTTCAG ACACAATGCT GACCCGTGAC ATTGTGAGGG AATGTCTTGA GAAAGAACCC
    ACAGACAGAA CAGATGATGA CATAGAACAG CTACTGGAAT TCATGCATCA GCTTCCAGCC
    TTTGTCAATA TGACCATGTC TGTGAGACGA AAGCTTTGCT CGGTCATGAT TTTTGAAGTT
    GTGGAACAGG CTGGCGCAAT CATCATTGAA GATGGGCAAG AACTGGACTC TTGGTATGTC
    ATTCTTAATG GGATTGTAGA AATCAGCCAT TCAGATGGCA GGATGGAAAA CCTTTGCATG
    GGAAATAGCT TTGGCATAAA CCCATCTTTA GAAACACAGT ACATGGATGG AGTAGTAAAA
    ACCAAAGCTG ATGATTGCCA GTTTGTGTGC ATAGCACAAC AGGACTACTT TCGGATATTA
    AACCATGTGG AAAAAAATAC CCACAAAGTC AAGGAAAAGG GGGAGATTGT TATGGTTAAT
    GAACACCGAG AACTGGATCG AAGTGGCACT CGAAAGGGAC ATATAGTAAT TCAGGGGACT
    CCAGAAAGAC TCATCTTGCA CCTAATAGAA GAGCACTCTG TTGTTGACCC AACATACATA
    GAGGATTTTC TATTAACATA CAGGACATTC CTTTCAAGTC CATTAGAGGT TGGGGACAAG
    CTTTTGGAGT GGTTTAAAGA TGACAGTCGT CGTGACAAGG TAACACGAGT TGTCCTTCTT
    TGGGTTAACA ACCACTTTAA TGATTTTGAA GGAGACCCTG CCATGACAAG GTTTTTGGAA
    GAATTTGAAC GGAATCTTGA AAATGCGAAA ATGAAGGGTC ATCTGCGACT GCTAAACATA
    GCCTGTGCTG CAAAGGCTAA ACTGAGACAG GTTACATTGC TTAAGCCTTC CCGTGAATCG
    CCTTTTTATT TCAACGTCGT TGGGGGAAGC GACAAAGGTT TCGGCATGTT TATTGAAAGT
    GTTGAGCCTG GAAGCAAAGC AACAGAAGCT GGATTAAAAC GCGGAGATCA GATTATGGAA
    GTCAATGGCC AAAATTTTGA GACTATCGTT TACGCCAAAG CCCTGGAGAT CTTGAAAAAC
    AATACACATC TTTCCATCAC TGTCAAGACC AACATTTTTG TGTTTAAAGA GCTTCTTAAT
    CGAATCGGGC AGGAGAAAAA AAATGGAGTC CCACACATCC CCAAAATTGC AGAGAAGAAG
    AACAATAGAT ATTCCATCCC TGATCTCCCC AATGATGTAG AACAGATGTT TCCACGGGAT
    CGCGCAAGCA AGAAAATGAA AGCCAATACG GTGTCTGGAG GGCGGAATCG CATCCGGAAG
    ATTTTGGACA AAACACGTTT TAGTATATTA CCACCTAAGC CATTCAGTGA TGGCCATGTT
    ATCCAGTCCC AGGATGACAG TATTGTTGGC ACCAGGCAGT GCAGGCACAG TGTGGCTATA
    ATGCCCATTC CGGGCACGCT CTCTTCAAGC AGTCCAGACT TGCTTCAAGC TACAACAAAC
    ATATTGGATT TCTCCAATCC TACTGATATT CCAGACCAAG TTATACGAAT ATTTAAAGCA
    GATCAGCAGT GCTGCTATAT TATCATCGGC AAAGACACTA CTGCAAAGGA GGTGGTGATA
    CAAGCACTTC ATGAATTCAG CCTTACTGGG TCAACAGACA CATACTCTCT CTGTGAGGTC
    TCGGTCAGTC CAGAAGGTGT CATTAAACAA AGACGGCTTC CGGATCAGCT TTCTAAACTG
    GCAGACAGAA TCCAGCTGAA TGGAAGGTAT TATTTGAAGA ACAACATGGA GACCGAAACG
    TTGTGTTCAG ATGAAGATGC TCAGGAGCTT CTGCGTGAAA GCCTCATCTC TCTCCTTCAG
    CTTAGCACTA TCGAGGTGGC AACACAGCTT TCTATGCGGG ACTTTGAACT CTTCCGCAAT
    ATTGAGCCCA CAGAATACAT AGATGACCTT TTCAAGATAG ATTCCAAGAC AAGTGCTGCT
    CATCTGAAAC AGTTTGAGGA AGTGATCAAC CAAGAGACAT TTTGGGTGGC CACGGAAATT
    CTAAGAGAAC CAAACCATTT GAAGAGAATG AAAATTGTGA AGCATTTCAT AAAGACTGCC
    CTCCATTGCA GAGAATGCAG AAATTTTAAC TCCATGTTTG CTATAATAAG TGGGCTCAAT
    CTTGCACCTG TGGCTCGGCT TCGAGGAACT TGGGAAAAGT TGCCCAGCAA ATACGAGAAG
    CTGCTTCGAG ACCTGCAAGA TCTCTTCGAC CCCTCAAGAA ATATGGCGAA ATACCGTAAT
    ATTCTAAGCA GTCAGAGCAT GCAGCCCCCC ATCATTCCTC TCTTCCCAGT CGTCAAGAAG
    GATATAACAT TTCTTCATGA AGGAAATGAT TCAAAAGTTG ATGGGTTAGT AAATTTTGAG
    AAGCTGCGAA TGATTGCAAA GGAGATTCGA CATGTTATTC GCATGACTTC TGCTAATATG
    GATCCAGCAG TTATGTTTAG GCAAAGGTCC CTAAGCCAAG GCAGCACAAA CTCTAACATG
    CTGGATGTTC AAGGAGGTGG TCACAAGAAA CGGGCTCGGC GAAGTTCATT CCTTAATGCT
    AAGAAGCTGT ATGAAGATGC ACAGATGTCA AGGAAGGTAA AACAGTACAT CTCCAACCTG
    AGTGTGGAAA TCGATGAGGA GAAGATCCAG CTAATGTCAC TGCAATGTGA ACCAGCCTAC
    AGCACTTTGG CAAAGAATTT AAGTGAGAGA AGATCTGCAA AATCTGACTT GTCTCCTGCA
    CCTTTGAGGC CATCAGGCCA AACTACCAAA GCTCACATCC ATCAGCAAAA CAGAATGAGC
    CAAGTATTGC AAGTCCCTAC AGTTAACTTA TATCCAATGC GAAAGAAAGC ACCAACCAAG
    GAACATCTTC CATGCACTTC AGGATCTTCA CAGAAAATAT CTGGTTTGAC AGATGAGGTT
    ACAGTTAAAG CACAGTTAGA AGACTCTGTT TCTCTTGTGT CATCATTGCA TTCCAGCCCA
    GCCGCATCCC CTCAAGGATC TCCACATAAA GTTGTTAACA TTTCAAGATC AGAGAATTCC
    AGTCATATGA ACCTCTCTGG ATCCTCCTCA TCATTAACCA GTGATCCCAA CCAGAAGATC
    TGCAGTCGGC GTGGCTATGG AATAGATATG TTAAGAGAGA CAGCAAAACA GAGGAAAGTT
    GGGACCTCTA GTGAGGCCTC TGAGGGAGGA AAAAGCAGAG AGATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa43153
    Clone ID Related Accession (Same CDS sequence) XM_018092130.1 , NM_001374789.1
    Accession Version NM_001374789.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4935bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-14
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 6
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAMC03010430.1 and AAMC03010433.1. On Oct 15, 2019 this sequence version replaced XM_018092130.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00862000, SAMN00862005 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGTCTGCCA GCATTGAACC CAGGTTTAAA GAAGCCTTGA GGAAAAAACC CAAGGAAAGG 
    ACTCAGGAGG ATCTGAAAAC TGTTTATTCA TATATGCATA GAATGGATAT ACTCTCACAT
    CTCAGAGAAC ATCAATTAAG ATTGATGTCT GCAACAGCAC GATATGAAAG ATACAGTGCA
    AATCAGGTTC TATTCTGCTC AGAAAGTTTG GCTAGATGTT GGTACATTCT TCTTTCTGGT
    TCAGTTCTGG TAAAAAACTC CATGTTTTTG CCTCCATGCA GTTTTGGCAA GCAGTCAGGT
    GGCAAAAGGG GTTGTGATTG CATTGTACTG GAACCTTCTG AAATGATTGT GGTGGAAAAT
    ACAGGAGAAA ACAATGATGG CATTCTGCAT AGGGAAACTT CTATTAGACA ATCCCGGCGT
    CGGTATCGAA AAATCAACCA GCATGGAGAA CGCCAAACAA TTACAGATAA TGGGGATGCT
    GCTAGTTATG TCTTGCTCCC CACAGACCTC TCCAAGATAC ACCTCACCGA CAGTCCCCAC
    CCACATGTTA CACATGTGCC ATCCAGCCAA TCAGGATGCA GCATTGCAAG CGACTCTGGA
    AGCAGCAGCT TATCAGATAT CTACCAGGCA ACAGAAAGTG AGATGGGGGA TGTTGACTTG
    ACAGGCCTGC CAGAGGCAGC TGTTGATTCT GAAGATGAAG AGGAAGATGA AGATATTGAG
    AGAGCTTCAG ACACAATGCT GACCCGTGAC ATTGTGAGGG AATGTCTTGA GAAAGAACCC
    ACAGACAGAA CAGATGATGA CATAGAACAG CTACTGGAAT TCATGCATCA GCTTCCAGCC
    TTTGTCAATA TGACCATGTC TGTGAGACGA AAGCTTTGCT CGGTCATGAT TTTTGAAGTT
    GTGGAACAGG CTGGCGCAAT CATCATTGAA GATGGGCAAG AACTGGACTC TTGGTATGTC
    ATTCTTAATG GGATTGTAGA AATCAGCCAT TCAGATGGCA GGATGGAAAA CCTTTGCATG
    GGAAATAGCT TTGGCATAAA CCCATCTTTA GAAACACAGT ACATGGATGG AGTAGTAAAA
    ACCAAAGCTG ATGATTGCCA GTTTGTGTGC ATAGCACAAC AGGACTACTT TCGGATATTA
    AACCATGTGG AAAAAAATAC CCACAAAGTC AAGGAAAAGG GGGAGATTGT TATGGTTAAT
    GAACACCGAG AACTGGATCG AAGTGGCACT CGAAAGGGAC ATATAGTAAT TCAGGGGACT
    CCAGAAAGAC TCATCTTGCA CCTAATAGAA GAGCACTCTG TTGTTGACCC AACATACATA
    GAGGATTTTC TATTAACATA CAGGACATTC CTTTCAAGTC CATTAGAGGT TGGGGACAAG
    CTTTTGGAGT GGTTTAAAGA TGACAGTCGT CGTGACAAGG TAACACGAGT TGTCCTTCTT
    TGGGTTAACA ACCACTTTAA TGATTTTGAA GGAGACCCTG CCATGACAAG GTTTTTGGAA
    GAATTTGAAC GGAATCTTGA AAATGCGAAA ATGAAGGGTC ATCTGCGACT GCTAAACATA
    GCCTGTGCTG CAAAGGCTAA ACTGAGACAG GTTACATTGC TTAAGCCTTC CCGTGAATCG
    CCTTTTTATT TCAACGTCGT TGGGGGAAGC GACAAAGGTT TCGGCATGTT TATTGAAAGT
    GTTGAGCCTG GAAGCAAAGC AACAGAAGCT GGATTAAAAC GCGGAGATCA GATTATGGAA
    GTCAATGGCC AAAATTTTGA GACTATCGTT TACGCCAAAG CCCTGGAGAT CTTGAAAAAC
    AATACACATC TTTCCATCAC TGTCAAGACC AACATTTTTG TGTTTAAAGA GCTTCTTAAT
    CGAATCGGGC AGGAGAAAAA AAATGGAGTC CCACACATCC CCAAAATTGC AGAGAAGAAG
    AACAATAGAT ATTCCATCCC TGATCTCCCC AATGATGTAG AACAGATGTT TCCACGGGAT
    CGCGCAAGCA AGAAAATGAA AGCCAATACG GTGTCTGGAG GGCGGAATCG CATCCGGAAG
    ATTTTGGACA AAACACGTTT TAGTATATTA CCACCTAAGC CATTCAGTGA TGGCCATGTT
    ATCCAGTCCC AGGATGACAG TATTGTTGGC ACCAGGCAGT GCAGGCACAG TGTGGCTATA
    ATGCCCATTC CGGGCACGCT CTCTTCAAGC AGTCCAGACT TGCTTCAAGC TACAACAAAC
    ATATTGGATT TCTCCAATCC TACTGCTTTT GGATTTTACT ATATTCCAGA CCAAGTTATA
    CGAATATTTA AAGCAGATCA GCAGTGCTGC TATATTATCA TCGGCAAAGA CACTACTGCA
    AAGGAGGTGG TGATACAAGC ACTTCATGAA TTCAGCCTTA CTGGGTCAAC AGACACATAC
    TCTCTCTGTG AGGTCTCGGT CAGTCCAGAA GGTGTCATTA AACAAAGACG GCTTCCGGAT
    CAGCTTTCTA AACTGGCAGA CAGAATCCAG CTGAATGGAA GGTATTATTT GAAGAACAAC
    ATGGAGACCG AAACGTTGTG TTCAGATGAA GATGCTCAGG AGCTTCTGCG TGAAAGCCTC
    ATCTCTCTCC TTCAGCTTAG CACTATCGAG GTGGCAACAC AGCTTTCTAT GCGGGACTTT
    GAACTCTTCC GCAATATTGA GCCCACAGAA TACATAGATG ACCTTTTCAA GATAGATTCC
    AAGACAAGTG CTGCTCATCT GAAACAGTTT GAGGAAGTGA TCAACCAAGA GACATTTTGG
    GTGGCCACGG AAATTCTAAG AGAACCAAAC CATTTGAAGA GAATGAAAAT TGTGAAGCAT
    TTCATAAAGA CTGCCCTCCA TTGCAGAGAA TGCAGAAATT TTAACTCCAT GTTTGCTATA
    ATAAGTGGGC TCAATCTTGC ACCTGTGGCT CGGCTTCGAG GAACTTGGGA AAAGTTGCCC
    AGCAAATACG AGAAGCTGCT TCGAGACCTG CAAGATCTCT TCGACCCCTC AAGAAATATG
    GCGAAATACC GTAATATTCT AAGCAGTCAG AGCATGCAGC CCCCCATCAT TCCTCTCTTC
    CCAGTCGTCA AGAAGGATAT AACATTTCTT CATGAAGGAA ATGATTCAAA AGTTGATGGG
    TTAGTAAATT TTGAGAAGCT GCGAATGATT GCAAAGGAGA TTCGACATGT TATTCGCATG
    ACTTCTGCTA ATATGGATCC AGCAGTTATG TTTAGGCAAA GGAAGAAGAG ATGGAGAAGT
    TTAGGGTCCC TAAGCCAAGG CAGCACAAAC TCTAACATGC TGGATGTTCA AGGAGGTGGT
    CACAAGAAAC GGGCTCGGCG AAGTTCATTC CTTAATGCTA AGAAGCTGTA TGAAGATGCA
    CAGATGTCAA GGAAGGTAAA ACAGTACATC TCCAACCTGA GTGTGGAAAT CGATGAGGAG
    AAGATCCAGC TAATGTCACT GCAATGTGAA CCAGCCTACA GCACTTTGGC AAAGAATTTA
    AGTGAGAGAA GATCTGCAAA ATCTGACTTG TCTCCTGCAC CTTTGAGGCC ATCAGGCCAA
    ACTACCAAAG CTCACATCCA TCAGCAAAAC AGAATGAGCC AAGTATTGCA AGTCCCTACA
    GTTAACTTAT ATCCAATGCG AAAGAAAGCA CCAACCAAGG AACATCTTCC ATGCACTTCA
    GGATCTTCAC AGAAAATATC TGGTTTGACA GATGAGGTTA CAGTTAAAGC ACAGTTAGAA
    GACTCTGTTT CTCTTGTGTC ATCATTGCAT TCCAGCCCAG CCGCATCCCC TCAAGGATCT
    CCACATAAAG TTGTTAACAT TTCAAGATCA GAGAATTCCA GTCATATGAA CCTCTCTGGA
    TCCTCCTCAT CATTAACCAG TGATCCCAAC CAGAAGATCT GCAGTCGGCG TGGCTATGGA
    ATAGGCTATG CCCATAACCC CTCGGCCAAA TCTGATAACT TCTCAGACTC AAGCAACAGT
    GAGATCTCGT CCCGATCAAG CCTCGTCAGC AACTGCTCAG TAGACTCCAT GTCGTTGACC
    CTGCAGGATG AAAAGTGTGT GCCTCATTCA CATGCTGAGT CAATGGGACT ATCAGAAAGA
    AAAGACGTCC ATCCGTCCTC TGAGTATAAC CAGCCCTGCA GTAGCTGGCT CCATGCAAGG
    CACCCTGCAG TCCGGAATTT CATAGCTTGC GCAAGCAGTG AGGACATAGC TCATGACCAC
    ATATCGGTAG AGCCGGTAGA CAGTGGTCGG GGCAGTTGGA CATCTTGTTC TAGCAGTTCA
    CATGACAATT TCCAGACTTT GCAGAAGGGA CTGGACCGCT TAAGCACTTA CCGCCAATCA
    CACATTGATT CCCCAATTGC TGAGACAGAG TCCGCAAACT GCAGCACAGA TGAAACATGT
    AGTGAAGACT GTACCAAAGG AAAAAGGCAC TCAAACAACA GTTTAGAACT AAATGAATCT
    CGGCAAAGTT GGGCTTCATC TAGCTCAATG TCAGATACTT ACGAGACGAA CTATGGCACA
    ATCAAGCGGA GAGTATTGGA CACAGAGGAA AAAACAGAGG CTTTGGGCCA ACAATTAGAT
    GTCTTTTATA AAACAGTTAC TTCCACTACT GAAAAAGGAC TGATAGTGTA CTGTGTCACC
    TCATCCCAGA AGGACAGTAG GTATAGGGAG CCACCTCCCA CTCCTCCAGG CTACCAGGGG
    ATATCATTAG CCGACATAAA GGAAGGACAA CCCCACCAAG CGCACTTAAA ACCTCCAGAT
    TATAGTGTGG CGATACAGAG GTCAAAGATG ATACATAAAG ACCTCTCTAG GCTTTCATCA
    GCCTGTGTTG AACCAGTCTC ATGTGTCTCA CAGAAGATGA TAACCCATTG GCATAACCGG
    CATCAGCCTT ATCCTAATGT ACAGGCTGAC AGAGATAGTA AAGAAGATGA AGATGAACAA
    ATGTCAGCAG TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001361718.1
    CDS1357..6291
    Translation

    Target ORF information:

    RefSeq Version NM_001374789.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 6 (rapgef6), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001374789.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGTCTGCCA GCATTGAACC CAGGTTTAAA GAAGCCTTGA GGAAAAAACC CAAGGAAAGG 
    ACTCAGGAGG ATCTGAAAAC TGTTTATTCA TATATGCATA GAATGGATAT ACTCTCACAT
    CTCAGAGAAC ATCAATTAAG ATTGATGTCT GCAACAGCAC GATATGAAAG ATACAGTGCA
    AATCAGGTTC TATTCTGCTC AGAAAGTTTG GCTAGATGTT GGTACATTCT TCTTTCTGGT
    TCAGTTCTGG TAAAAAACTC CATGTTTTTG CCTCCATGCA GTTTTGGCAA GCAGTCAGGT
    GGCAAAAGGG GTTGTGATTG CATTGTACTG GAACCTTCTG AAATGATTGT GGTGGAAAAT
    ACAGGAGAAA ACAATGATGG CATTCTGCAT AGGGAAACTT CTATTAGACA ATCCCGGCGT
    CGGTATCGAA AAATCAACCA GCATGGAGAA CGCCAAACAA TTACAGATAA TGGGGATGCT
    GCTAGTTATG TCTTGCTCCC CACAGACCTC TCCAAGATAC ACCTCACCGA CAGTCCCCAC
    CCACATGTTA CACATGTGCC ATCCAGCCAA TCAGGATGCA GCATTGCAAG CGACTCTGGA
    AGCAGCAGCT TATCAGATAT CTACCAGGCA ACAGAAAGTG AGATGGGGGA TGTTGACTTG
    ACAGGCCTGC CAGAGGCAGC TGTTGATTCT GAAGATGAAG AGGAAGATGA AGATATTGAG
    AGAGCTTCAG ACACAATGCT GACCCGTGAC ATTGTGAGGG AATGTCTTGA GAAAGAACCC
    ACAGACAGAA CAGATGATGA CATAGAACAG CTACTGGAAT TCATGCATCA GCTTCCAGCC
    TTTGTCAATA TGACCATGTC TGTGAGACGA AAGCTTTGCT CGGTCATGAT TTTTGAAGTT
    GTGGAACAGG CTGGCGCAAT CATCATTGAA GATGGGCAAG AACTGGACTC TTGGTATGTC
    ATTCTTAATG GGATTGTAGA AATCAGCCAT TCAGATGGCA GGATGGAAAA CCTTTGCATG
    GGAAATAGCT TTGGCATAAA CCCATCTTTA GAAACACAGT ACATGGATGG AGTAGTAAAA
    ACCAAAGCTG ATGATTGCCA GTTTGTGTGC ATAGCACAAC AGGACTACTT TCGGATATTA
    AACCATGTGG AAAAAAATAC CCACAAAGTC AAGGAAAAGG GGGAGATTGT TATGGTTAAT
    GAACACCGAG AACTGGATCG AAGTGGCACT CGAAAGGGAC ATATAGTAAT TCAGGGGACT
    CCAGAAAGAC TCATCTTGCA CCTAATAGAA GAGCACTCTG TTGTTGACCC AACATACATA
    GAGGATTTTC TATTAACATA CAGGACATTC CTTTCAAGTC CATTAGAGGT TGGGGACAAG
    CTTTTGGAGT GGTTTAAAGA TGACAGTCGT CGTGACAAGG TAACACGAGT TGTCCTTCTT
    TGGGTTAACA ACCACTTTAA TGATTTTGAA GGAGACCCTG CCATGACAAG GTTTTTGGAA
    GAATTTGAAC GGAATCTTGA AAATGCGAAA ATGAAGGGTC ATCTGCGACT GCTAAACATA
    GCCTGTGCTG CAAAGGCTAA ACTGAGACAG GTTACATTGC TTAAGCCTTC CCGTGAATCG
    CCTTTTTATT TCAACGTCGT TGGGGGAAGC GACAAAGGTT TCGGCATGTT TATTGAAAGT
    GTTGAGCCTG GAAGCAAAGC AACAGAAGCT GGATTAAAAC GCGGAGATCA GATTATGGAA
    GTCAATGGCC AAAATTTTGA GACTATCGTT TACGCCAAAG CCCTGGAGAT CTTGAAAAAC
    AATACACATC TTTCCATCAC TGTCAAGACC AACATTTTTG TGTTTAAAGA GCTTCTTAAT
    CGAATCGGGC AGGAGAAAAA AAATGGAGTC CCACACATCC CCAAAATTGC AGAGAAGAAG
    AACAATAGAT ATTCCATCCC TGATCTCCCC AATGATGTAG AACAGATGTT TCCACGGGAT
    CGCGCAAGCA AGAAAATGAA AGCCAATACG GTGTCTGGAG GGCGGAATCG CATCCGGAAG
    ATTTTGGACA AAACACGTTT TAGTATATTA CCACCTAAGC CATTCAGTGA TGGCCATGTT
    ATCCAGTCCC AGGATGACAG TATTGTTGGC ACCAGGCAGT GCAGGCACAG TGTGGCTATA
    ATGCCCATTC CGGGCACGCT CTCTTCAAGC AGTCCAGACT TGCTTCAAGC TACAACAAAC
    ATATTGGATT TCTCCAATCC TACTGCTTTT GGATTTTACT ATATTCCAGA CCAAGTTATA
    CGAATATTTA AAGCAGATCA GCAGTGCTGC TATATTATCA TCGGCAAAGA CACTACTGCA
    AAGGAGGTGG TGATACAAGC ACTTCATGAA TTCAGCCTTA CTGGGTCAAC AGACACATAC
    TCTCTCTGTG AGGTCTCGGT CAGTCCAGAA GGTGTCATTA AACAAAGACG GCTTCCGGAT
    CAGCTTTCTA AACTGGCAGA CAGAATCCAG CTGAATGGAA GGTATTATTT GAAGAACAAC
    ATGGAGACCG AAACGTTGTG TTCAGATGAA GATGCTCAGG AGCTTCTGCG TGAAAGCCTC
    ATCTCTCTCC TTCAGCTTAG CACTATCGAG GTGGCAACAC AGCTTTCTAT GCGGGACTTT
    GAACTCTTCC GCAATATTGA GCCCACAGAA TACATAGATG ACCTTTTCAA GATAGATTCC
    AAGACAAGTG CTGCTCATCT GAAACAGTTT GAGGAAGTGA TCAACCAAGA GACATTTTGG
    GTGGCCACGG AAATTCTAAG AGAACCAAAC CATTTGAAGA GAATGAAAAT TGTGAAGCAT
    TTCATAAAGA CTGCCCTCCA TTGCAGAGAA TGCAGAAATT TTAACTCCAT GTTTGCTATA
    ATAAGTGGGC TCAATCTTGC ACCTGTGGCT CGGCTTCGAG GAACTTGGGA AAAGTTGCCC
    AGCAAATACG AGAAGCTGCT TCGAGACCTG CAAGATCTCT TCGACCCCTC AAGAAATATG
    GCGAAATACC GTAATATTCT AAGCAGTCAG AGCATGCAGC CCCCCATCAT TCCTCTCTTC
    CCAGTCGTCA AGAAGGATAT AACATTTCTT CATGAAGGAA ATGATTCAAA AGTTGATGGG
    TTAGTAAATT TTGAGAAGCT GCGAATGATT GCAAAGGAGA TTCGACATGT TATTCGCATG
    ACTTCTGCTA ATATGGATCC AGCAGTTATG TTTAGGCAAA GGAAGAAGAG ATGGAGAAGT
    TTAGGGTCCC TAAGCCAAGG CAGCACAAAC TCTAACATGC TGGATGTTCA AGGAGGTGGT
    CACAAGAAAC GGGCTCGGCG AAGTTCATTC CTTAATGCTA AGAAGCTGTA TGAAGATGCA
    CAGATGTCAA GGAAGGTAAA ACAGTACATC TCCAACCTGA GTGTGGAAAT CGATGAGGAG
    AAGATCCAGC TAATGTCACT GCAATGTGAA CCAGCCTACA GCACTTTGGC AAAGAATTTA
    AGTGAGAGAA GATCTGCAAA ATCTGACTTG TCTCCTGCAC CTTTGAGGCC ATCAGGCCAA
    ACTACCAAAG CTCACATCCA TCAGCAAAAC AGAATGAGCC AAGTATTGCA AGTCCCTACA
    GTTAACTTAT ATCCAATGCG AAAGAAAGCA CCAACCAAGG AACATCTTCC ATGCACTTCA
    GGATCTTCAC AGAAAATATC TGGTTTGACA GATGAGGTTA CAGTTAAAGC ACAGTTAGAA
    GACTCTGTTT CTCTTGTGTC ATCATTGCAT TCCAGCCCAG CCGCATCCCC TCAAGGATCT
    CCACATAAAG TTGTTAACAT TTCAAGATCA GAGAATTCCA GTCATATGAA CCTCTCTGGA
    TCCTCCTCAT CATTAACCAG TGATCCCAAC CAGAAGATCT GCAGTCGGCG TGGCTATGGA
    ATAGGCTATG CCCATAACCC CTCGGCCAAA TCTGATAACT TCTCAGACTC AAGCAACAGT
    GAGATCTCGT CCCGATCAAG CCTCGTCAGC AACTGCTCAG TAGACTCCAT GTCGTTGACC
    CTGCAGGATG AAAAGTGTGT GCCTCATTCA CATGCTGAGT CAATGGGACT ATCAGAAAGA
    AAAGACGTCC ATCCGTCCTC TGAGTATAAC CAGCCCTGCA GTAGCTGGCT CCATGCAAGG
    CACCCTGCAG TCCGGAATTT CATAGCTTGC GCAAGCAGTG AGGACATAGC TCATGACCAC
    ATATCGGTAG AGCCGGTAGA CAGTGGTCGG GGCAGTTGGA CATCTTGTTC TAGCAGTTCA
    CATGACAATT TCCAGACTTT GCAGAAGGGA CTGGACCGCT TAAGCACTTA CCGCCAATCA
    CACATTGATT CCCCAATTGC TGAGACAGAG TCCGCAAACT GCAGCACAGA TGAAACATGT
    AGTGAAGACT GTACCAAAGG AAAAAGGCAC TCAAACAACA GTTTAGAACT AAATGAATCT
    CGGCAAAGTT GGGCTTCATC TAGCTCAATG TCAGATACTT ACGAGACGAA CTATGGCACA
    ATCAAGCGGA GAGTATTGGA CACAGAGGAA AAAACAGAGG CTTTGGGCCA ACAATTAGAT
    GTCTTTTATA AAACAGTTAC TTCCACTACT GAAAAAGGAC TGATAGTGTA CTGTGTCACC
    TCATCCCAGA AGGACAGTAG GTATAGGGAG CCACCTCCCA CTCCTCCAGG CTACCAGGGG
    ATATCATTAG CCGACATAAA GGAAGGACAA CCCCACCAAG CGCACTTAAA ACCTCCAGAT
    TATAGTGTGG CGATACAGAG GTCAAAGATG ATACATAAAG ACCTCTCTAG GCTTTCATCA
    GCCTGTGTTG AACCAGTCTC ATGTGTCTCA CAGAAGATGA TAACCCATTG GCATAACCGG
    CATCAGCCTT ATCCTAATGT ACAGGCTGAC AGAGATAGTA AAGAAGATGA AGATGAACAA
    ATGTCAGCAG TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa43153
    Clone ID Related Accession (Same CDS sequence) XM_018092130.1 , NM_001374789.1
    Accession Version XM_018092130.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4935bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGTCTGCCA GCATTGAACC CAGGTTTAAA GAAGCCTTGA GGAAAAAACC CAAGGAAAGG 
    ACTCAGGAGG ATCTGAAAAC TGTTTATTCA TATATGCATA GAATGGATAT ACTCTCACAT
    CTCAGAGAAC ATCAATTAAG ATTGATGTCT GCAACAGCAC GATATGAAAG ATACAGTGCA
    AATCAGGTTC TATTCTGCTC AGAAAGTTTG GCTAGATGTT GGTACATTCT TCTTTCTGGT
    TCAGTTCTGG TAAAAAACTC CATGTTTTTG CCTCCATGCA GTTTTGGCAA GCAGTCAGGT
    GGCAAAAGGG GTTGTGATTG CATTGTACTG GAACCTTCTG AAATGATTGT GGTGGAAAAT
    ACAGGAGAAA ACAATGATGG CATTCTGCAT AGGGAAACTT CTATTAGACA ATCCCGGCGT
    CGGTATCGAA AAATCAACCA GCATGGAGAA CGCCAAACAA TTACAGATAA TGGGGATGCT
    GCTAGTTATG TCTTGCTCCC CACAGACCTC TCCAAGATAC ACCTCACCGA CAGTCCCCAC
    CCACATGTTA CACATGTGCC ATCCAGCCAA TCAGGATGCA GCATTGCAAG CGACTCTGGA
    AGCAGCAGCT TATCAGATAT CTACCAGGCA ACAGAAAGTG AGATGGGGGA TGTTGACTTG
    ACAGGCCTGC CAGAGGCAGC TGTTGATTCT GAAGATGAAG AGGAAGATGA AGATATTGAG
    AGAGCTTCAG ACACAATGCT GACCCGTGAC ATTGTGAGGG AATGTCTTGA GAAAGAACCC
    ACAGACAGAA CAGATGATGA CATAGAACAG CTACTGGAAT TCATGCATCA GCTTCCAGCC
    TTTGTCAATA TGACCATGTC TGTGAGACGA AAGCTTTGCT CGGTCATGAT TTTTGAAGTT
    GTGGAACAGG CTGGCGCAAT CATCATTGAA GATGGGCAAG AACTGGACTC TTGGTATGTC
    ATTCTTAATG GGATTGTAGA AATCAGCCAT TCAGATGGCA GGATGGAAAA CCTTTGCATG
    GGAAATAGCT TTGGCATAAA CCCATCTTTA GAAACACAGT ACATGGATGG AGTAGTAAAA
    ACCAAAGCTG ATGATTGCCA GTTTGTGTGC ATAGCACAAC AGGACTACTT TCGGATATTA
    AACCATGTGG AAAAAAATAC CCACAAAGTC AAGGAAAAGG GGGAGATTGT TATGGTTAAT
    GAACACCGAG AACTGGATCG AAGTGGCACT CGAAAGGGAC ATATAGTAAT TCAGGGGACT
    CCAGAAAGAC TCATCTTGCA CCTAATAGAA GAGCACTCTG TTGTTGACCC AACATACATA
    GAGGATTTTC TATTAACATA CAGGACATTC CTTTCAAGTC CATTAGAGGT TGGGGACAAG
    CTTTTGGAGT GGTTTAAAGA TGACAGTCGT CGTGACAAGG TAACACGAGT TGTCCTTCTT
    TGGGTTAACA ACCACTTTAA TGATTTTGAA GGAGACCCTG CCATGACAAG GTTTTTGGAA
    GAATTTGAAC GGAATCTTGA AAATGCGAAA ATGAAGGGTC ATCTGCGACT GCTAAACATA
    GCCTGTGCTG CAAAGGCTAA ACTGAGACAG GTTACATTGC TTAAGCCTTC CCGTGAATCG
    CCTTTTTATT TCAACGTCGT TGGGGGAAGC GACAAAGGTT TCGGCATGTT TATTGAAAGT
    GTTGAGCCTG GAAGCAAAGC AACAGAAGCT GGATTAAAAC GCGGAGATCA GATTATGGAA
    GTCAATGGCC AAAATTTTGA GACTATCGTT TACGCCAAAG CCCTGGAGAT CTTGAAAAAC
    AATACACATC TTTCCATCAC TGTCAAGACC AACATTTTTG TGTTTAAAGA GCTTCTTAAT
    CGAATCGGGC AGGAGAAAAA AAATGGAGTC CCACACATCC CCAAAATTGC AGAGAAGAAG
    AACAATAGAT ATTCCATCCC TGATCTCCCC AATGATGTAG AACAGATGTT TCCACGGGAT
    CGCGCAAGCA AGAAAATGAA AGCCAATACG GTGTCTGGAG GGCGGAATCG CATCCGGAAG
    ATTTTGGACA AAACACGTTT TAGTATATTA CCACCTAAGC CATTCAGTGA TGGCCATGTT
    ATCCAGTCCC AGGATGACAG TATTGTTGGC ACCAGGCAGT GCAGGCACAG TGTGGCTATA
    ATGCCCATTC CGGGCACGCT CTCTTCAAGC AGTCCAGACT TGCTTCAAGC TACAACAAAC
    ATATTGGATT TCTCCAATCC TACTGCTTTT GGATTTTACT ATATTCCAGA CCAAGTTATA
    CGAATATTTA AAGCAGATCA GCAGTGCTGC TATATTATCA TCGGCAAAGA CACTACTGCA
    AAGGAGGTGG TGATACAAGC ACTTCATGAA TTCAGCCTTA CTGGGTCAAC AGACACATAC
    TCTCTCTGTG AGGTCTCGGT CAGTCCAGAA GGTGTCATTA AACAAAGACG GCTTCCGGAT
    CAGCTTTCTA AACTGGCAGA CAGAATCCAG CTGAATGGAA GGTATTATTT GAAGAACAAC
    ATGGAGACCG AAACGTTGTG TTCAGATGAA GATGCTCAGG AGCTTCTGCG TGAAAGCCTC
    ATCTCTCTCC TTCAGCTTAG CACTATCGAG GTGGCAACAC AGCTTTCTAT GCGGGACTTT
    GAACTCTTCC GCAATATTGA GCCCACAGAA TACATAGATG ACCTTTTCAA GATAGATTCC
    AAGACAAGTG CTGCTCATCT GAAACAGTTT GAGGAAGTGA TCAACCAAGA GACATTTTGG
    GTGGCCACGG AAATTCTAAG AGAACCAAAC CATTTGAAGA GAATGAAAAT TGTGAAGCAT
    TTCATAAAGA CTGCCCTCCA TTGCAGAGAA TGCAGAAATT TTAACTCCAT GTTTGCTATA
    ATAAGTGGGC TCAATCTTGC ACCTGTGGCT CGGCTTCGAG GAACTTGGGA AAAGTTGCCC
    AGCAAATACG AGAAGCTGCT TCGAGACCTG CAAGATCTCT TCGACCCCTC AAGAAATATG
    GCGAAATACC GTAATATTCT AAGCAGTCAG AGCATGCAGC CCCCCATCAT TCCTCTCTTC
    CCAGTCGTCA AGAAGGATAT AACATTTCTT CATGAAGGAA ATGATTCAAA AGTTGATGGG
    TTAGTAAATT TTGAGAAGCT GCGAATGATT GCAAAGGAGA TTCGACATGT TATTCGCATG
    ACTTCTGCTA ATATGGATCC AGCAGTTATG TTTAGGCAAA GGAAGAAGAG ATGGAGAAGT
    TTAGGGTCCC TAAGCCAAGG CAGCACAAAC TCTAACATGC TGGATGTTCA AGGAGGTGGT
    CACAAGAAAC GGGCTCGGCG AAGTTCATTC CTTAATGCTA AGAAGCTGTA TGAAGATGCA
    CAGATGTCAA GGAAGGTAAA ACAGTACATC TCCAACCTGA GTGTGGAAAT CGATGAGGAG
    AAGATCCAGC TAATGTCACT GCAATGTGAA CCAGCCTACA GCACTTTGGC AAAGAATTTA
    AGTGAGAGAA GATCTGCAAA ATCTGACTTG TCTCCTGCAC CTTTGAGGCC ATCAGGCCAA
    ACTACCAAAG CTCACATCCA TCAGCAAAAC AGAATGAGCC AAGTATTGCA AGTCCCTACA
    GTTAACTTAT ATCCAATGCG AAAGAAAGCA CCAACCAAGG AACATCTTCC ATGCACTTCA
    GGATCTTCAC AGAAAATATC TGGTTTGACA GATGAGGTTA CAGTTAAAGC ACAGTTAGAA
    GACTCTGTTT CTCTTGTGTC ATCATTGCAT TCCAGCCCAG CCGCATCCCC TCAAGGATCT
    CCACATAAAG TTGTTAACAT TTCAAGATCA GAGAATTCCA GTCATATGAA CCTCTCTGGA
    TCCTCCTCAT CATTAACCAG TGATCCCAAC CAGAAGATCT GCAGTCGGCG TGGCTATGGA
    ATAGGCTATG CCCATAACCC CTCGGCCAAA TCTGATAACT TCTCAGACTC AAGCAACAGT
    GAGATCTCGT CCCGATCAAG CCTCGTCAGC AACTGCTCAG TAGACTCCAT GTCGTTGACC
    CTGCAGGATG AAAAGTGTGT GCCTCATTCA CATGCTGAGT CAATGGGACT ATCAGAAAGA
    AAAGACGTCC ATCCGTCCTC TGAGTATAAC CAGCCCTGCA GTAGCTGGCT CCATGCAAGG
    CACCCTGCAG TCCGGAATTT CATAGCTTGC GCAAGCAGTG AGGACATAGC TCATGACCAC
    ATATCGGTAG AGCCGGTAGA CAGTGGTCGG GGCAGTTGGA CATCTTGTTC TAGCAGTTCA
    CATGACAATT TCCAGACTTT GCAGAAGGGA CTGGACCGCT TAAGCACTTA CCGCCAATCA
    CACATTGATT CCCCAATTGC TGAGACAGAG TCCGCAAACT GCAGCACAGA TGAAACATGT
    AGTGAAGACT GTACCAAAGG AAAAAGGCAC TCAAACAACA GTTTAGAACT AAATGAATCT
    CGGCAAAGTT GGGCTTCATC TAGCTCAATG TCAGATACTT ACGAGACGAA CTATGGCACA
    ATCAAGCGGA GAGTATTGGA CACAGAGGAA AAAACAGAGG CTTTGGGCCA ACAATTAGAT
    GTCTTTTATA AAACAGTTAC TTCCACTACT GAAAAAGGAC TGATAGTGTA CTGTGTCACC
    TCATCCCAGA AGGACAGTAG GTATAGGGAG CCACCTCCCA CTCCTCCAGG CTACCAGGGG
    ATATCATTAG CCGACATAAA GGAAGGACAA CCCCACCAAG CGCACTTAAA ACCTCCAGAT
    TATAGTGTGG CGATACAGAG GTCAAAGATG ATACATAAAG ACCTCTCTAG GCTTTCATCA
    GCCTGTGTTG AACCAGTCTC ATGTGTCTCA CAGAAGATGA TAACCCATTG GCATAACCGG
    CATCAGCCTT ATCCTAATGT ACAGGCTGAC AGAGATAGTA AAGAAGATGA AGATGAACAA
    ATGTCAGCAG TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947619.1
    CDS1357..6291
    Translation

    Target ORF information:

    RefSeq Version XM_018092130.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 6 (rapgef6), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092130.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGTCTGCCA GCATTGAACC CAGGTTTAAA GAAGCCTTGA GGAAAAAACC CAAGGAAAGG 
    ACTCAGGAGG ATCTGAAAAC TGTTTATTCA TATATGCATA GAATGGATAT ACTCTCACAT
    CTCAGAGAAC ATCAATTAAG ATTGATGTCT GCAACAGCAC GATATGAAAG ATACAGTGCA
    AATCAGGTTC TATTCTGCTC AGAAAGTTTG GCTAGATGTT GGTACATTCT TCTTTCTGGT
    TCAGTTCTGG TAAAAAACTC CATGTTTTTG CCTCCATGCA GTTTTGGCAA GCAGTCAGGT
    GGCAAAAGGG GTTGTGATTG CATTGTACTG GAACCTTCTG AAATGATTGT GGTGGAAAAT
    ACAGGAGAAA ACAATGATGG CATTCTGCAT AGGGAAACTT CTATTAGACA ATCCCGGCGT
    CGGTATCGAA AAATCAACCA GCATGGAGAA CGCCAAACAA TTACAGATAA TGGGGATGCT
    GCTAGTTATG TCTTGCTCCC CACAGACCTC TCCAAGATAC ACCTCACCGA CAGTCCCCAC
    CCACATGTTA CACATGTGCC ATCCAGCCAA TCAGGATGCA GCATTGCAAG CGACTCTGGA
    AGCAGCAGCT TATCAGATAT CTACCAGGCA ACAGAAAGTG AGATGGGGGA TGTTGACTTG
    ACAGGCCTGC CAGAGGCAGC TGTTGATTCT GAAGATGAAG AGGAAGATGA AGATATTGAG
    AGAGCTTCAG ACACAATGCT GACCCGTGAC ATTGTGAGGG AATGTCTTGA GAAAGAACCC
    ACAGACAGAA CAGATGATGA CATAGAACAG CTACTGGAAT TCATGCATCA GCTTCCAGCC
    TTTGTCAATA TGACCATGTC TGTGAGACGA AAGCTTTGCT CGGTCATGAT TTTTGAAGTT
    GTGGAACAGG CTGGCGCAAT CATCATTGAA GATGGGCAAG AACTGGACTC TTGGTATGTC
    ATTCTTAATG GGATTGTAGA AATCAGCCAT TCAGATGGCA GGATGGAAAA CCTTTGCATG
    GGAAATAGCT TTGGCATAAA CCCATCTTTA GAAACACAGT ACATGGATGG AGTAGTAAAA
    ACCAAAGCTG ATGATTGCCA GTTTGTGTGC ATAGCACAAC AGGACTACTT TCGGATATTA
    AACCATGTGG AAAAAAATAC CCACAAAGTC AAGGAAAAGG GGGAGATTGT TATGGTTAAT
    GAACACCGAG AACTGGATCG AAGTGGCACT CGAAAGGGAC ATATAGTAAT TCAGGGGACT
    CCAGAAAGAC TCATCTTGCA CCTAATAGAA GAGCACTCTG TTGTTGACCC AACATACATA
    GAGGATTTTC TATTAACATA CAGGACATTC CTTTCAAGTC CATTAGAGGT TGGGGACAAG
    CTTTTGGAGT GGTTTAAAGA TGACAGTCGT CGTGACAAGG TAACACGAGT TGTCCTTCTT
    TGGGTTAACA ACCACTTTAA TGATTTTGAA GGAGACCCTG CCATGACAAG GTTTTTGGAA
    GAATTTGAAC GGAATCTTGA AAATGCGAAA ATGAAGGGTC ATCTGCGACT GCTAAACATA
    GCCTGTGCTG CAAAGGCTAA ACTGAGACAG GTTACATTGC TTAAGCCTTC CCGTGAATCG
    CCTTTTTATT TCAACGTCGT TGGGGGAAGC GACAAAGGTT TCGGCATGTT TATTGAAAGT
    GTTGAGCCTG GAAGCAAAGC AACAGAAGCT GGATTAAAAC GCGGAGATCA GATTATGGAA
    GTCAATGGCC AAAATTTTGA GACTATCGTT TACGCCAAAG CCCTGGAGAT CTTGAAAAAC
    AATACACATC TTTCCATCAC TGTCAAGACC AACATTTTTG TGTTTAAAGA GCTTCTTAAT
    CGAATCGGGC AGGAGAAAAA AAATGGAGTC CCACACATCC CCAAAATTGC AGAGAAGAAG
    AACAATAGAT ATTCCATCCC TGATCTCCCC AATGATGTAG AACAGATGTT TCCACGGGAT
    CGCGCAAGCA AGAAAATGAA AGCCAATACG GTGTCTGGAG GGCGGAATCG CATCCGGAAG
    ATTTTGGACA AAACACGTTT TAGTATATTA CCACCTAAGC CATTCAGTGA TGGCCATGTT
    ATCCAGTCCC AGGATGACAG TATTGTTGGC ACCAGGCAGT GCAGGCACAG TGTGGCTATA
    ATGCCCATTC CGGGCACGCT CTCTTCAAGC AGTCCAGACT TGCTTCAAGC TACAACAAAC
    ATATTGGATT TCTCCAATCC TACTGCTTTT GGATTTTACT ATATTCCAGA CCAAGTTATA
    CGAATATTTA AAGCAGATCA GCAGTGCTGC TATATTATCA TCGGCAAAGA CACTACTGCA
    AAGGAGGTGG TGATACAAGC ACTTCATGAA TTCAGCCTTA CTGGGTCAAC AGACACATAC
    TCTCTCTGTG AGGTCTCGGT CAGTCCAGAA GGTGTCATTA AACAAAGACG GCTTCCGGAT
    CAGCTTTCTA AACTGGCAGA CAGAATCCAG CTGAATGGAA GGTATTATTT GAAGAACAAC
    ATGGAGACCG AAACGTTGTG TTCAGATGAA GATGCTCAGG AGCTTCTGCG TGAAAGCCTC
    ATCTCTCTCC TTCAGCTTAG CACTATCGAG GTGGCAACAC AGCTTTCTAT GCGGGACTTT
    GAACTCTTCC GCAATATTGA GCCCACAGAA TACATAGATG ACCTTTTCAA GATAGATTCC
    AAGACAAGTG CTGCTCATCT GAAACAGTTT GAGGAAGTGA TCAACCAAGA GACATTTTGG
    GTGGCCACGG AAATTCTAAG AGAACCAAAC CATTTGAAGA GAATGAAAAT TGTGAAGCAT
    TTCATAAAGA CTGCCCTCCA TTGCAGAGAA TGCAGAAATT TTAACTCCAT GTTTGCTATA
    ATAAGTGGGC TCAATCTTGC ACCTGTGGCT CGGCTTCGAG GAACTTGGGA AAAGTTGCCC
    AGCAAATACG AGAAGCTGCT TCGAGACCTG CAAGATCTCT TCGACCCCTC AAGAAATATG
    GCGAAATACC GTAATATTCT AAGCAGTCAG AGCATGCAGC CCCCCATCAT TCCTCTCTTC
    CCAGTCGTCA AGAAGGATAT AACATTTCTT CATGAAGGAA ATGATTCAAA AGTTGATGGG
    TTAGTAAATT TTGAGAAGCT GCGAATGATT GCAAAGGAGA TTCGACATGT TATTCGCATG
    ACTTCTGCTA ATATGGATCC AGCAGTTATG TTTAGGCAAA GGAAGAAGAG ATGGAGAAGT
    TTAGGGTCCC TAAGCCAAGG CAGCACAAAC TCTAACATGC TGGATGTTCA AGGAGGTGGT
    CACAAGAAAC GGGCTCGGCG AAGTTCATTC CTTAATGCTA AGAAGCTGTA TGAAGATGCA
    CAGATGTCAA GGAAGGTAAA ACAGTACATC TCCAACCTGA GTGTGGAAAT CGATGAGGAG
    AAGATCCAGC TAATGTCACT GCAATGTGAA CCAGCCTACA GCACTTTGGC AAAGAATTTA
    AGTGAGAGAA GATCTGCAAA ATCTGACTTG TCTCCTGCAC CTTTGAGGCC ATCAGGCCAA
    ACTACCAAAG CTCACATCCA TCAGCAAAAC AGAATGAGCC AAGTATTGCA AGTCCCTACA
    GTTAACTTAT ATCCAATGCG AAAGAAAGCA CCAACCAAGG AACATCTTCC ATGCACTTCA
    GGATCTTCAC AGAAAATATC TGGTTTGACA GATGAGGTTA CAGTTAAAGC ACAGTTAGAA
    GACTCTGTTT CTCTTGTGTC ATCATTGCAT TCCAGCCCAG CCGCATCCCC TCAAGGATCT
    CCACATAAAG TTGTTAACAT TTCAAGATCA GAGAATTCCA GTCATATGAA CCTCTCTGGA
    TCCTCCTCAT CATTAACCAG TGATCCCAAC CAGAAGATCT GCAGTCGGCG TGGCTATGGA
    ATAGGCTATG CCCATAACCC CTCGGCCAAA TCTGATAACT TCTCAGACTC AAGCAACAGT
    GAGATCTCGT CCCGATCAAG CCTCGTCAGC AACTGCTCAG TAGACTCCAT GTCGTTGACC
    CTGCAGGATG AAAAGTGTGT GCCTCATTCA CATGCTGAGT CAATGGGACT ATCAGAAAGA
    AAAGACGTCC ATCCGTCCTC TGAGTATAAC CAGCCCTGCA GTAGCTGGCT CCATGCAAGG
    CACCCTGCAG TCCGGAATTT CATAGCTTGC GCAAGCAGTG AGGACATAGC TCATGACCAC
    ATATCGGTAG AGCCGGTAGA CAGTGGTCGG GGCAGTTGGA CATCTTGTTC TAGCAGTTCA
    CATGACAATT TCCAGACTTT GCAGAAGGGA CTGGACCGCT TAAGCACTTA CCGCCAATCA
    CACATTGATT CCCCAATTGC TGAGACAGAG TCCGCAAACT GCAGCACAGA TGAAACATGT
    AGTGAAGACT GTACCAAAGG AAAAAGGCAC TCAAACAACA GTTTAGAACT AAATGAATCT
    CGGCAAAGTT GGGCTTCATC TAGCTCAATG TCAGATACTT ACGAGACGAA CTATGGCACA
    ATCAAGCGGA GAGTATTGGA CACAGAGGAA AAAACAGAGG CTTTGGGCCA ACAATTAGAT
    GTCTTTTATA AAACAGTTAC TTCCACTACT GAAAAAGGAC TGATAGTGTA CTGTGTCACC
    TCATCCCAGA AGGACAGTAG GTATAGGGAG CCACCTCCCA CTCCTCCAGG CTACCAGGGG
    ATATCATTAG CCGACATAAA GGAAGGACAA CCCCACCAAG CGCACTTAAA ACCTCCAGAT
    TATAGTGTGG CGATACAGAG GTCAAAGATG ATACATAAAG ACCTCTCTAG GCTTTCATCA
    GCCTGTGTTG AACCAGTCTC ATGTGTCTCA CAGAAGATGA TAACCCATTG GCATAACCGG
    CATCAGCCTT ATCCTAATGT ACAGGCTGAC AGAGATAGTA AAGAAGATGA AGATGAACAA
    ATGTCAGCAG TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa43154
    Clone ID Related Accession (Same CDS sequence) XM_018092131.2 , XM_018092131.1
    Accession Version XM_018092131.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4920bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018092131.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTGCCA GCATTGAACC CAGGTTTAAA GAAGCCTTGA GGAAAAAACC CAAGGAAAGG 
    ACTCAGGAGG ATCTGAAAAC TGTTTATTCA TATATGCATA GAATGGATAT ACTCTCACAT
    CTCAGAGAAC ATCAATTAAG ATTGATGTCT GCAACAGCAC GATATGAAAG ATACAGTGCA
    AATCAGGTTC TATTCTGCTC AGAAAGTTTG GCTAGATGTT GGTACATTCT TCTTTCTGGT
    TCAGTTCTGG TAAAAAACTC CATGTTTTTG CCTCCATGCA GTTTTGGCAA GCAGTCAGGT
    GGCAAAAGGG GTTGTGATTG CATTGTACTG GAACCTTCTG AAATGATTGT GGTGGAAAAT
    ACAGGAGAAA ACAATGATGG CATTCTGCAT AGGGAAACTT CTATTAGACA ATCCCGGCGT
    CGGTATCGAA AAATCAACCA GCATGGAGAA CGCCAAACAA TTACAGATAA TGGGGATGCT
    GCTAGTTATG TCTTGCTCCC CACAGACCTC TCCAAGATAC ACCTCACCGA CAGTCCCCAC
    CCACATGTTA CACATGTGCC ATCCAGCCAA TCAGGATGCA GCATTGCAAG CGACTCTGGA
    AGCAGCAGCT TATCAGATAT CTACCAGGCA ACAGAAAGTG AGATGGGGGA TGTTGACTTG
    ACAGGCCTGC CAGAGGCAGC TGTTGATTCT GAAGATGAAG AGGAAGATGA AGATATTGAG
    AGAGCTTCAG ACACAATGCT GACCCGTGAC ATTGTGAGGG AATGTCTTGA GAAAGAACCC
    ACAGACAGAA CAGATGATGA CATAGAACAG CTACTGGAAT TCATGCATCA GCTTCCAGCC
    TTTGTCAATA TGACCATGTC TGTGAGACGA AAGCTTTGCT CGGTCATGAT TTTTGAAGTT
    GTGGAACAGG CTGGCGCAAT CATCATTGAA GATGGGCAAG AACTGGACTC TTGGTATGTC
    ATTCTTAATG GGATTGTAGA AATCAGCCAT TCAGATGGCA GGATGGAAAA CCTTTGCATG
    GGAAATAGCT TTGGCATAAA CCCATCTTTA GAAACACAGT ACATGGATGG AGTAGTAAAA
    ACCAAAGCTG ATGATTGCCA GTTTGTGTGC ATAGCACAAC AGGACTACTT TCGGATATTA
    AACCATGTGG AAAAAAATAC CCACAAAGTC AAGGAAAAGG GGGAGATTGT TATGGTTAAT
    GAACACCGAG AACTGGATCG AAGTGGCACT CGAAAGGGAC ATATAGTAAT TCAGGGGACT
    CCAGAAAGAC TCATCTTGCA CCTAATAGAA GAGCACTCTG TTGTTGACCC AACATACATA
    GAGGATTTTC TATTAACATA CAGGACATTC CTTTCAAGTC CATTAGAGGT TGGGGACAAG
    CTTTTGGAGT GGTTTAAAGA TGACAGTCGT CGTGACAAGG TAACACGAGT TGTCCTTCTT
    TGGGTTAACA ACCACTTTAA TGATTTTGAA GGAGACCCTG CCATGACAAG GTTTTTGGAA
    GAATTTGAAC GGAATCTTGA AAATGCGAAA ATGAAGGGTC ATCTGCGACT GCTAAACATA
    GCCTGTGCTG CAAAGGCTAA ACTGAGACAG GTTACATTGC TTAAGCCTTC CCGTGAATCG
    CCTTTTTATT TCAACGTCGT TGGGGGAAGC GACAAAGGTT TCGGCATGTT TATTGAAAGT
    GTTGAGCCTG GAAGCAAAGC AACAGAAGCT GGATTAAAAC GCGGAGATCA GATTATGGAA
    GTCAATGGCC AAAATTTTGA GACTATCGTT TACGCCAAAG CCCTGGAGAT CTTGAAAAAC
    AATACACATC TTTCCATCAC TGTCAAGACC AACATTTTTG TGTTTAAAGA GCTTCTTAAT
    CGAATCGGGC AGGAGAAAAA AAATGGAGTC CCACACATCC CCAAAATTGC AGAGAAGAAG
    AACAATAGAT ATTCCATCCC TGATCTCCCC AATGATGTAG AACAGATGTT TCCACGGGAT
    CGCGCAAGCA AGAAAATGAA AGCCAATACG GTGTCTGGAG GGCGGAATCG CATCCGGAAG
    ATTTTGGACA AAACACGTTT TAGTATATTA CCACCTAAGC CATTCAGTGA TGGCCATGTT
    ATCCAGTCCC AGGATGACAG TATTGTTGGC ACCAGGCAGT GCAGGCACAG TGTGGCTATA
    ATGCCCATTC CGGGCACGCT CTCTTCAAGC AGTCCAGACT TGCTTCAAGC TACAACAAAC
    ATATTGGATT TCTCCAATCC TACTGATATT CCAGACCAAG TTATACGAAT ATTTAAAGCA
    GATCAGCAGT GCTGCTATAT TATCATCGGC AAAGACACTA CTGCAAAGGA GGTGGTGATA
    CAAGCACTTC ATGAATTCAG CCTTACTGGG TCAACAGACA CATACTCTCT CTGTGAGGTC
    TCGGTCAGTC CAGAAGGTGT CATTAAACAA AGACGGCTTC CGGATCAGCT TTCTAAACTG
    GCAGACAGAA TCCAGCTGAA TGGAAGGTAT TATTTGAAGA ACAACATGGA GACCGAAACG
    TTGTGTTCAG ATGAAGATGC TCAGGAGCTT CTGCGTGAAA GCCTCATCTC TCTCCTTCAG
    CTTAGCACTA TCGAGGTGGC AACACAGCTT TCTATGCGGG ACTTTGAACT CTTCCGCAAT
    ATTGAGCCCA CAGAATACAT AGATGACCTT TTCAAGATAG ATTCCAAGAC AAGTGCTGCT
    CATCTGAAAC AGTTTGAGGA AGTGATCAAC CAAGAGACAT TTTGGGTGGC CACGGAAATT
    CTAAGAGAAC CAAACCATTT GAAGAGAATG AAAATTGTGA AGCATTTCAT AAAGACTGCC
    CTCCATTGCA GAGAATGCAG AAATTTTAAC TCCATGTTTG CTATAATAAG TGGGCTCAAT
    CTTGCACCTG TGGCTCGGCT TCGAGGAACT TGGGAAAAGT TGCCCAGCAA ATACGAGAAG
    CTGCTTCGAG ACCTGCAAGA TCTCTTCGAC CCCTCAAGAA ATATGGCGAA ATACCGTAAT
    ATTCTAAGCA GTCAGAGCAT GCAGCCCCCC ATCATTCCTC TCTTCCCAGT CGTCAAGAAG
    GATATAACAT TTCTTCATGA AGGAAATGAT TCAAAAGTTG ATGGGTTAGT AAATTTTGAG
    AAGCTGCGAA TGATTGCAAA GGAGATTCGA CATGTTATTC GCATGACTTC TGCTAATATG
    GATCCAGCAG TTATGTTTAG GCAAAGGAAG AAGAGATGGA GAAGTTTAGG GTCCCTAAGC
    CAAGGCAGCA CAAACTCTAA CATGCTGGAT GTTCAAGGAG GTGGTCACAA GAAACGGGCT
    CGGCGAAGTT CATTCCTTAA TGCTAAGAAG CTGTATGAAG ATGCACAGAT GTCAAGGAAG
    GTAAAACAGT ACATCTCCAA CCTGAGTGTG GAAATCGATG AGGAGAAGAT CCAGCTAATG
    TCACTGCAAT GTGAACCAGC CTACAGCACT TTGGCAAAGA ATTTAAGTGA GAGAAGATCT
    GCAAAATCTG ACTTGTCTCC TGCACCTTTG AGGCCATCAG GCCAAACTAC CAAAGCTCAC
    ATCCATCAGC AAAACAGAAT GAGCCAAGTA TTGCAAGTCC CTACAGTTAA CTTATATCCA
    ATGCGAAAGA AAGCACCAAC CAAGGAACAT CTTCCATGCA CTTCAGGATC TTCACAGAAA
    ATATCTGGTT TGACAGATGA GGTTACAGTT AAAGCACAGT TAGAAGACTC TGTTTCTCTT
    GTGTCATCAT TGCATTCCAG CCCAGCCGCA TCCCCTCAAG GATCTCCACA TAAAGTTGTT
    AACATTTCAA GATCAGAGAA TTCCAGTCAT ATGAACCTCT CTGGATCCTC CTCATCATTA
    ACCAGTGATC CCAACCAGAA GATCTGCAGT CGGCGTGGCT ATGGAATAGG CTATGCCCAT
    AACCCCTCGG CCAAATCTGA TAACTTCTCA GACTCAAGCA ACAGTGAGAT CTCGTCCCGA
    TCAAGCCTCG TCAGCAACTG CTCAGTAGAC TCCATGTCGT TGACCCTGCA GGATGAAAAG
    TGTGTGCCTC ATTCACATGC TGAGTCAATG GGACTATCAG AAAGAAAAGA CGTCCATCCG
    TCCTCTGAGT ATAACCAGCC CTGCAGTAGC TGGCTCCATG CAAGGCACCC TGCAGTCCGG
    AATTTCATAG CTTGCGCAAG CAGTGAGGAC ATAGCTCATG ACCACATATC GGTAGAGCCG
    GTAGACAGTG GTCGGGGCAG TTGGACATCT TGTTCTAGCA GTTCACATGA CAATTTCCAG
    ACTTTGCAGA AGGGACTGGA CCGCTTAAGC ACTTACCGCC AATCACACAT TGATTCCCCA
    ATTGCTGAGA CAGAGTCCGC AAACTGCAGC ACAGATGAAA CATGTAGTGA AGACTGTACC
    AAAGGAAAAA GGCACTCAAA CAACAGTTTA GAACTAAATG AATCTCGGCA AAGTTGGGCT
    TCATCTAGCT CAATGTCAGA TACTTACGAG ACGAACTATG GCACAATCAA GCGGAGAGTA
    TTGGACACAG AGGAAAAAAC AGAGGCTTTG GGCCAACAAT TAGATGTCTT TTATAAAACA
    GTTACTTCCA CTACTGAAAA AGGACTGATA GTGTACTGTG TCACCTCATC CCAGAAGGAC
    AGTAGGTATA GGGAGCCACC TCCCACTCCT CCAGGCTACC AGGGGATATC ATTAGCCGAC
    ATAAAGGAAG GACAACCCCA CCAAGCGCAC TTAAAACCTC CAGATTATAG TGTGGCGATA
    CAGAGGTCAA AGATGATACA TAAAGACCTC TCTAGGCTTT CATCAGCCTG TGTTGAACCA
    GTCTCATGTG TCTCACAGAA GATGATAACC CATTGGCATA ACCGGCATCA GCCTTATCCT
    AATGTACAGG CTGACAGAGA TAGTAAAGAA GATGAAGATG AACAAATGTC AGCAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947620.1
    CDS485..5404
    Translation

    Target ORF information:

    RefSeq Version XM_018092131.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 6 (rapgef6), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092131.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTGCCA GCATTGAACC CAGGTTTAAA GAAGCCTTGA GGAAAAAACC CAAGGAAAGG 
    ACTCAGGAGG ATCTGAAAAC TGTTTATTCA TATATGCATA GAATGGATAT ACTCTCACAT
    CTCAGAGAAC ATCAATTAAG ATTGATGTCT GCAACAGCAC GATATGAAAG ATACAGTGCA
    AATCAGGTTC TATTCTGCTC AGAAAGTTTG GCTAGATGTT GGTACATTCT TCTTTCTGGT
    TCAGTTCTGG TAAAAAACTC CATGTTTTTG CCTCCATGCA GTTTTGGCAA GCAGTCAGGT
    GGCAAAAGGG GTTGTGATTG CATTGTACTG GAACCTTCTG AAATGATTGT GGTGGAAAAT
    ACAGGAGAAA ACAATGATGG CATTCTGCAT AGGGAAACTT CTATTAGACA ATCCCGGCGT
    CGGTATCGAA AAATCAACCA GCATGGAGAA CGCCAAACAA TTACAGATAA TGGGGATGCT
    GCTAGTTATG TCTTGCTCCC CACAGACCTC TCCAAGATAC ACCTCACCGA CAGTCCCCAC
    CCACATGTTA CACATGTGCC ATCCAGCCAA TCAGGATGCA GCATTGCAAG CGACTCTGGA
    AGCAGCAGCT TATCAGATAT CTACCAGGCA ACAGAAAGTG AGATGGGGGA TGTTGACTTG
    ACAGGCCTGC CAGAGGCAGC TGTTGATTCT GAAGATGAAG AGGAAGATGA AGATATTGAG
    AGAGCTTCAG ACACAATGCT GACCCGTGAC ATTGTGAGGG AATGTCTTGA GAAAGAACCC
    ACAGACAGAA CAGATGATGA CATAGAACAG CTACTGGAAT TCATGCATCA GCTTCCAGCC
    TTTGTCAATA TGACCATGTC TGTGAGACGA AAGCTTTGCT CGGTCATGAT TTTTGAAGTT
    GTGGAACAGG CTGGCGCAAT CATCATTGAA GATGGGCAAG AACTGGACTC TTGGTATGTC
    ATTCTTAATG GGATTGTAGA AATCAGCCAT TCAGATGGCA GGATGGAAAA CCTTTGCATG
    GGAAATAGCT TTGGCATAAA CCCATCTTTA GAAACACAGT ACATGGATGG AGTAGTAAAA
    ACCAAAGCTG ATGATTGCCA GTTTGTGTGC ATAGCACAAC AGGACTACTT TCGGATATTA
    AACCATGTGG AAAAAAATAC CCACAAAGTC AAGGAAAAGG GGGAGATTGT TATGGTTAAT
    GAACACCGAG AACTGGATCG AAGTGGCACT CGAAAGGGAC ATATAGTAAT TCAGGGGACT
    CCAGAAAGAC TCATCTTGCA CCTAATAGAA GAGCACTCTG TTGTTGACCC AACATACATA
    GAGGATTTTC TATTAACATA CAGGACATTC CTTTCAAGTC CATTAGAGGT TGGGGACAAG
    CTTTTGGAGT GGTTTAAAGA TGACAGTCGT CGTGACAAGG TAACACGAGT TGTCCTTCTT
    TGGGTTAACA ACCACTTTAA TGATTTTGAA GGAGACCCTG CCATGACAAG GTTTTTGGAA
    GAATTTGAAC GGAATCTTGA AAATGCGAAA ATGAAGGGTC ATCTGCGACT GCTAAACATA
    GCCTGTGCTG CAAAGGCTAA ACTGAGACAG GTTACATTGC TTAAGCCTTC CCGTGAATCG
    CCTTTTTATT TCAACGTCGT TGGGGGAAGC GACAAAGGTT TCGGCATGTT TATTGAAAGT
    GTTGAGCCTG GAAGCAAAGC AACAGAAGCT GGATTAAAAC GCGGAGATCA GATTATGGAA
    GTCAATGGCC AAAATTTTGA GACTATCGTT TACGCCAAAG CCCTGGAGAT CTTGAAAAAC
    AATACACATC TTTCCATCAC TGTCAAGACC AACATTTTTG TGTTTAAAGA GCTTCTTAAT
    CGAATCGGGC AGGAGAAAAA AAATGGAGTC CCACACATCC CCAAAATTGC AGAGAAGAAG
    AACAATAGAT ATTCCATCCC TGATCTCCCC AATGATGTAG AACAGATGTT TCCACGGGAT
    CGCGCAAGCA AGAAAATGAA AGCCAATACG GTGTCTGGAG GGCGGAATCG CATCCGGAAG
    ATTTTGGACA AAACACGTTT TAGTATATTA CCACCTAAGC CATTCAGTGA TGGCCATGTT
    ATCCAGTCCC AGGATGACAG TATTGTTGGC ACCAGGCAGT GCAGGCACAG TGTGGCTATA
    ATGCCCATTC CGGGCACGCT CTCTTCAAGC AGTCCAGACT TGCTTCAAGC TACAACAAAC
    ATATTGGATT TCTCCAATCC TACTGATATT CCAGACCAAG TTATACGAAT ATTTAAAGCA
    GATCAGCAGT GCTGCTATAT TATCATCGGC AAAGACACTA CTGCAAAGGA GGTGGTGATA
    CAAGCACTTC ATGAATTCAG CCTTACTGGG TCAACAGACA CATACTCTCT CTGTGAGGTC
    TCGGTCAGTC CAGAAGGTGT CATTAAACAA AGACGGCTTC CGGATCAGCT TTCTAAACTG
    GCAGACAGAA TCCAGCTGAA TGGAAGGTAT TATTTGAAGA ACAACATGGA GACCGAAACG
    TTGTGTTCAG ATGAAGATGC TCAGGAGCTT CTGCGTGAAA GCCTCATCTC TCTCCTTCAG
    CTTAGCACTA TCGAGGTGGC AACACAGCTT TCTATGCGGG ACTTTGAACT CTTCCGCAAT
    ATTGAGCCCA CAGAATACAT AGATGACCTT TTCAAGATAG ATTCCAAGAC AAGTGCTGCT
    CATCTGAAAC AGTTTGAGGA AGTGATCAAC CAAGAGACAT TTTGGGTGGC CACGGAAATT
    CTAAGAGAAC CAAACCATTT GAAGAGAATG AAAATTGTGA AGCATTTCAT AAAGACTGCC
    CTCCATTGCA GAGAATGCAG AAATTTTAAC TCCATGTTTG CTATAATAAG TGGGCTCAAT
    CTTGCACCTG TGGCTCGGCT TCGAGGAACT TGGGAAAAGT TGCCCAGCAA ATACGAGAAG
    CTGCTTCGAG ACCTGCAAGA TCTCTTCGAC CCCTCAAGAA ATATGGCGAA ATACCGTAAT
    ATTCTAAGCA GTCAGAGCAT GCAGCCCCCC ATCATTCCTC TCTTCCCAGT CGTCAAGAAG
    GATATAACAT TTCTTCATGA AGGAAATGAT TCAAAAGTTG ATGGGTTAGT AAATTTTGAG
    AAGCTGCGAA TGATTGCAAA GGAGATTCGA CATGTTATTC GCATGACTTC TGCTAATATG
    GATCCAGCAG TTATGTTTAG GCAAAGGAAG AAGAGATGGA GAAGTTTAGG GTCCCTAAGC
    CAAGGCAGCA CAAACTCTAA CATGCTGGAT GTTCAAGGAG GTGGTCACAA GAAACGGGCT
    CGGCGAAGTT CATTCCTTAA TGCTAAGAAG CTGTATGAAG ATGCACAGAT GTCAAGGAAG
    GTAAAACAGT ACATCTCCAA CCTGAGTGTG GAAATCGATG AGGAGAAGAT CCAGCTAATG
    TCACTGCAAT GTGAACCAGC CTACAGCACT TTGGCAAAGA ATTTAAGTGA GAGAAGATCT
    GCAAAATCTG ACTTGTCTCC TGCACCTTTG AGGCCATCAG GCCAAACTAC CAAAGCTCAC
    ATCCATCAGC AAAACAGAAT GAGCCAAGTA TTGCAAGTCC CTACAGTTAA CTTATATCCA
    ATGCGAAAGA AAGCACCAAC CAAGGAACAT CTTCCATGCA CTTCAGGATC TTCACAGAAA
    ATATCTGGTT TGACAGATGA GGTTACAGTT AAAGCACAGT TAGAAGACTC TGTTTCTCTT
    GTGTCATCAT TGCATTCCAG CCCAGCCGCA TCCCCTCAAG GATCTCCACA TAAAGTTGTT
    AACATTTCAA GATCAGAGAA TTCCAGTCAT ATGAACCTCT CTGGATCCTC CTCATCATTA
    ACCAGTGATC CCAACCAGAA GATCTGCAGT CGGCGTGGCT ATGGAATAGG CTATGCCCAT
    AACCCCTCGG CCAAATCTGA TAACTTCTCA GACTCAAGCA ACAGTGAGAT CTCGTCCCGA
    TCAAGCCTCG TCAGCAACTG CTCAGTAGAC TCCATGTCGT TGACCCTGCA GGATGAAAAG
    TGTGTGCCTC ATTCACATGC TGAGTCAATG GGACTATCAG AAAGAAAAGA CGTCCATCCG
    TCCTCTGAGT ATAACCAGCC CTGCAGTAGC TGGCTCCATG CAAGGCACCC TGCAGTCCGG
    AATTTCATAG CTTGCGCAAG CAGTGAGGAC ATAGCTCATG ACCACATATC GGTAGAGCCG
    GTAGACAGTG GTCGGGGCAG TTGGACATCT TGTTCTAGCA GTTCACATGA CAATTTCCAG
    ACTTTGCAGA AGGGACTGGA CCGCTTAAGC ACTTACCGCC AATCACACAT TGATTCCCCA
    ATTGCTGAGA CAGAGTCCGC AAACTGCAGC ACAGATGAAA CATGTAGTGA AGACTGTACC
    AAAGGAAAAA GGCACTCAAA CAACAGTTTA GAACTAAATG AATCTCGGCA AAGTTGGGCT
    TCATCTAGCT CAATGTCAGA TACTTACGAG ACGAACTATG GCACAATCAA GCGGAGAGTA
    TTGGACACAG AGGAAAAAAC AGAGGCTTTG GGCCAACAAT TAGATGTCTT TTATAAAACA
    GTTACTTCCA CTACTGAAAA AGGACTGATA GTGTACTGTG TCACCTCATC CCAGAAGGAC
    AGTAGGTATA GGGAGCCACC TCCCACTCCT CCAGGCTACC AGGGGATATC ATTAGCCGAC
    ATAAAGGAAG GACAACCCCA CCAAGCGCAC TTAAAACCTC CAGATTATAG TGTGGCGATA
    CAGAGGTCAA AGATGATACA TAAAGACCTC TCTAGGCTTT CATCAGCCTG TGTTGAACCA
    GTCTCATGTG TCTCACAGAA GATGATAACC CATTGGCATA ACCGGCATCA GCCTTATCCT
    AATGTACAGG CTGACAGAGA TAGTAAAGAA GATGAAGATG AACAAATGTC AGCAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa43154
    Clone ID Related Accession (Same CDS sequence) XM_018092131.2 , XM_018092131.1
    Accession Version XM_018092131.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4920bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 6 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTGCCA GCATTGAACC CAGGTTTAAA GAAGCCTTGA GGAAAAAACC CAAGGAAAGG 
    ACTCAGGAGG ATCTGAAAAC TGTTTATTCA TATATGCATA GAATGGATAT ACTCTCACAT
    CTCAGAGAAC ATCAATTAAG ATTGATGTCT GCAACAGCAC GATATGAAAG ATACAGTGCA
    AATCAGGTTC TATTCTGCTC AGAAAGTTTG GCTAGATGTT GGTACATTCT TCTTTCTGGT
    TCAGTTCTGG TAAAAAACTC CATGTTTTTG CCTCCATGCA GTTTTGGCAA GCAGTCAGGT
    GGCAAAAGGG GTTGTGATTG CATTGTACTG GAACCTTCTG AAATGATTGT GGTGGAAAAT
    ACAGGAGAAA ACAATGATGG CATTCTGCAT AGGGAAACTT CTATTAGACA ATCCCGGCGT
    CGGTATCGAA AAATCAACCA GCATGGAGAA CGCCAAACAA TTACAGATAA TGGGGATGCT
    GCTAGTTATG TCTTGCTCCC CACAGACCTC TCCAAGATAC ACCTCACCGA CAGTCCCCAC
    CCACATGTTA CACATGTGCC ATCCAGCCAA TCAGGATGCA GCATTGCAAG CGACTCTGGA
    AGCAGCAGCT TATCAGATAT CTACCAGGCA ACAGAAAGTG AGATGGGGGA TGTTGACTTG
    ACAGGCCTGC CAGAGGCAGC TGTTGATTCT GAAGATGAAG AGGAAGATGA AGATATTGAG
    AGAGCTTCAG ACACAATGCT GACCCGTGAC ATTGTGAGGG AATGTCTTGA GAAAGAACCC
    ACAGACAGAA CAGATGATGA CATAGAACAG CTACTGGAAT TCATGCATCA GCTTCCAGCC
    TTTGTCAATA TGACCATGTC TGTGAGACGA AAGCTTTGCT CGGTCATGAT TTTTGAAGTT
    GTGGAACAGG CTGGCGCAAT CATCATTGAA GATGGGCAAG AACTGGACTC TTGGTATGTC
    ATTCTTAATG GGATTGTAGA AATCAGCCAT TCAGATGGCA GGATGGAAAA CCTTTGCATG
    GGAAATAGCT TTGGCATAAA CCCATCTTTA GAAACACAGT ACATGGATGG AGTAGTAAAA
    ACCAAAGCTG ATGATTGCCA GTTTGTGTGC ATAGCACAAC AGGACTACTT TCGGATATTA
    AACCATGTGG AAAAAAATAC CCACAAAGTC AAGGAAAAGG GGGAGATTGT TATGGTTAAT
    GAACACCGAG AACTGGATCG AAGTGGCACT CGAAAGGGAC ATATAGTAAT TCAGGGGACT
    CCAGAAAGAC TCATCTTGCA CCTAATAGAA GAGCACTCTG TTGTTGACCC AACATACATA
    GAGGATTTTC TATTAACATA CAGGACATTC CTTTCAAGTC CATTAGAGGT TGGGGACAAG
    CTTTTGGAGT GGTTTAAAGA TGACAGTCGT CGTGACAAGG TAACACGAGT TGTCCTTCTT
    TGGGTTAACA ACCACTTTAA TGATTTTGAA GGAGACCCTG CCATGACAAG GTTTTTGGAA
    GAATTTGAAC GGAATCTTGA AAATGCGAAA ATGAAGGGTC ATCTGCGACT GCTAAACATA
    GCCTGTGCTG CAAAGGCTAA ACTGAGACAG GTTACATTGC TTAAGCCTTC CCGTGAATCG
    CCTTTTTATT TCAACGTCGT TGGGGGAAGC GACAAAGGTT TCGGCATGTT TATTGAAAGT
    GTTGAGCCTG GAAGCAAAGC AACAGAAGCT GGATTAAAAC GCGGAGATCA GATTATGGAA
    GTCAATGGCC AAAATTTTGA GACTATCGTT TACGCCAAAG CCCTGGAGAT CTTGAAAAAC
    AATACACATC TTTCCATCAC TGTCAAGACC AACATTTTTG TGTTTAAAGA GCTTCTTAAT
    CGAATCGGGC AGGAGAAAAA AAATGGAGTC CCACACATCC CCAAAATTGC AGAGAAGAAG
    AACAATAGAT ATTCCATCCC TGATCTCCCC AATGATGTAG AACAGATGTT TCCACGGGAT
    CGCGCAAGCA AGAAAATGAA AGCCAATACG GTGTCTGGAG GGCGGAATCG CATCCGGAAG
    ATTTTGGACA AAACACGTTT TAGTATATTA CCACCTAAGC CATTCAGTGA TGGCCATGTT
    ATCCAGTCCC AGGATGACAG TATTGTTGGC ACCAGGCAGT GCAGGCACAG TGTGGCTATA
    ATGCCCATTC CGGGCACGCT CTCTTCAAGC AGTCCAGACT TGCTTCAAGC TACAACAAAC
    ATATTGGATT TCTCCAATCC TACTGATATT CCAGACCAAG TTATACGAAT ATTTAAAGCA
    GATCAGCAGT GCTGCTATAT TATCATCGGC AAAGACACTA CTGCAAAGGA GGTGGTGATA
    CAAGCACTTC ATGAATTCAG CCTTACTGGG TCAACAGACA CATACTCTCT CTGTGAGGTC
    TCGGTCAGTC CAGAAGGTGT CATTAAACAA AGACGGCTTC CGGATCAGCT TTCTAAACTG
    GCAGACAGAA TCCAGCTGAA TGGAAGGTAT TATTTGAAGA ACAACATGGA GACCGAAACG
    TTGTGTTCAG ATGAAGATGC TCAGGAGCTT CTGCGTGAAA GCCTCATCTC TCTCCTTCAG
    CTTAGCACTA TCGAGGTGGC AACACAGCTT TCTATGCGGG ACTTTGAACT CTTCCGCAAT
    ATTGAGCCCA CAGAATACAT AGATGACCTT TTCAAGATAG ATTCCAAGAC AAGTGCTGCT
    CATCTGAAAC AGTTTGAGGA AGTGATCAAC CAAGAGACAT TTTGGGTGGC CACGGAAATT
    CTAAGAGAAC CAAACCATTT GAAGAGAATG AAAATTGTGA AGCATTTCAT AAAGACTGCC
    CTCCATTGCA GAGAATGCAG AAATTTTAAC TCCATGTTTG CTATAATAAG TGGGCTCAAT
    CTTGCACCTG TGGCTCGGCT TCGAGGAACT TGGGAAAAGT TGCCCAGCAA ATACGAGAAG
    CTGCTTCGAG ACCTGCAAGA TCTCTTCGAC CCCTCAAGAA ATATGGCGAA ATACCGTAAT
    ATTCTAAGCA GTCAGAGCAT GCAGCCCCCC ATCATTCCTC TCTTCCCAGT CGTCAAGAAG
    GATATAACAT TTCTTCATGA AGGAAATGAT TCAAAAGTTG ATGGGTTAGT AAATTTTGAG
    AAGCTGCGAA TGATTGCAAA GGAGATTCGA CATGTTATTC GCATGACTTC TGCTAATATG
    GATCCAGCAG TTATGTTTAG GCAAAGGAAG AAGAGATGGA GAAGTTTAGG GTCCCTAAGC
    CAAGGCAGCA CAAACTCTAA CATGCTGGAT GTTCAAGGAG GTGGTCACAA GAAACGGGCT
    CGGCGAAGTT CATTCCTTAA TGCTAAGAAG CTGTATGAAG ATGCACAGAT GTCAAGGAAG
    GTAAAACAGT ACATCTCCAA CCTGAGTGTG GAAATCGATG AGGAGAAGAT CCAGCTAATG
    TCACTGCAAT GTGAACCAGC CTACAGCACT TTGGCAAAGA ATTTAAGTGA GAGAAGATCT
    GCAAAATCTG ACTTGTCTCC TGCACCTTTG AGGCCATCAG GCCAAACTAC CAAAGCTCAC
    ATCCATCAGC AAAACAGAAT GAGCCAAGTA TTGCAAGTCC CTACAGTTAA CTTATATCCA
    ATGCGAAAGA AAGCACCAAC CAAGGAACAT CTTCCATGCA CTTCAGGATC TTCACAGAAA
    ATATCTGGTT TGACAGATGA GGTTACAGTT AAAGCACAGT TAGAAGACTC TGTTTCTCTT
    GTGTCATCAT TGCATTCCAG CCCAGCCGCA TCCCCTCAAG GATCTCCACA TAAAGTTGTT
    AACATTTCAA GATCAGAGAA TTCCAGTCAT ATGAACCTCT CTGGATCCTC CTCATCATTA
    ACCAGTGATC CCAACCAGAA GATCTGCAGT CGGCGTGGCT ATGGAATAGG CTATGCCCAT
    AACCCCTCGG CCAAATCTGA TAACTTCTCA GACTCAAGCA ACAGTGAGAT CTCGTCCCGA
    TCAAGCCTCG TCAGCAACTG CTCAGTAGAC TCCATGTCGT TGACCCTGCA GGATGAAAAG
    TGTGTGCCTC ATTCACATGC TGAGTCAATG GGACTATCAG AAAGAAAAGA CGTCCATCCG
    TCCTCTGAGT ATAACCAGCC CTGCAGTAGC TGGCTCCATG CAAGGCACCC TGCAGTCCGG
    AATTTCATAG CTTGCGCAAG CAGTGAGGAC ATAGCTCATG ACCACATATC GGTAGAGCCG
    GTAGACAGTG GTCGGGGCAG TTGGACATCT TGTTCTAGCA GTTCACATGA CAATTTCCAG
    ACTTTGCAGA AGGGACTGGA CCGCTTAAGC ACTTACCGCC AATCACACAT TGATTCCCCA
    ATTGCTGAGA CAGAGTCCGC AAACTGCAGC ACAGATGAAA CATGTAGTGA AGACTGTACC
    AAAGGAAAAA GGCACTCAAA CAACAGTTTA GAACTAAATG AATCTCGGCA AAGTTGGGCT
    TCATCTAGCT CAATGTCAGA TACTTACGAG ACGAACTATG GCACAATCAA GCGGAGAGTA
    TTGGACACAG AGGAAAAAAC AGAGGCTTTG GGCCAACAAT TAGATGTCTT TTATAAAACA
    GTTACTTCCA CTACTGAAAA AGGACTGATA GTGTACTGTG TCACCTCATC CCAGAAGGAC
    AGTAGGTATA GGGAGCCACC TCCCACTCCT CCAGGCTACC AGGGGATATC ATTAGCCGAC
    ATAAAGGAAG GACAACCCCA CCAAGCGCAC TTAAAACCTC CAGATTATAG TGTGGCGATA
    CAGAGGTCAA AGATGATACA TAAAGACCTC TCTAGGCTTT CATCAGCCTG TGTTGAACCA
    GTCTCATGTG TCTCACAGAA GATGATAACC CATTGGCATA ACCGGCATCA GCCTTATCCT
    AATGTACAGG CTGACAGAGA TAGTAAAGAA GATGAAGATG AACAAATGTC AGCAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947620.1
    CDS1356..6275
    Translation

    Target ORF information:

    RefSeq Version XM_018092131.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 6 (rapgef6), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092131.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTGCCA GCATTGAACC CAGGTTTAAA GAAGCCTTGA GGAAAAAACC CAAGGAAAGG 
    ACTCAGGAGG ATCTGAAAAC TGTTTATTCA TATATGCATA GAATGGATAT ACTCTCACAT
    CTCAGAGAAC ATCAATTAAG ATTGATGTCT GCAACAGCAC GATATGAAAG ATACAGTGCA
    AATCAGGTTC TATTCTGCTC AGAAAGTTTG GCTAGATGTT GGTACATTCT TCTTTCTGGT
    TCAGTTCTGG TAAAAAACTC CATGTTTTTG CCTCCATGCA GTTTTGGCAA GCAGTCAGGT
    GGCAAAAGGG GTTGTGATTG CATTGTACTG GAACCTTCTG AAATGATTGT GGTGGAAAAT
    ACAGGAGAAA ACAATGATGG CATTCTGCAT AGGGAAACTT CTATTAGACA ATCCCGGCGT
    CGGTATCGAA AAATCAACCA GCATGGAGAA CGCCAAACAA TTACAGATAA TGGGGATGCT
    GCTAGTTATG TCTTGCTCCC CACAGACCTC TCCAAGATAC ACCTCACCGA CAGTCCCCAC
    CCACATGTTA CACATGTGCC ATCCAGCCAA TCAGGATGCA GCATTGCAAG CGACTCTGGA
    AGCAGCAGCT TATCAGATAT CTACCAGGCA ACAGAAAGTG AGATGGGGGA TGTTGACTTG
    ACAGGCCTGC CAGAGGCAGC TGTTGATTCT GAAGATGAAG AGGAAGATGA AGATATTGAG
    AGAGCTTCAG ACACAATGCT GACCCGTGAC ATTGTGAGGG AATGTCTTGA GAAAGAACCC
    ACAGACAGAA CAGATGATGA CATAGAACAG CTACTGGAAT TCATGCATCA GCTTCCAGCC
    TTTGTCAATA TGACCATGTC TGTGAGACGA AAGCTTTGCT CGGTCATGAT TTTTGAAGTT
    GTGGAACAGG CTGGCGCAAT CATCATTGAA GATGGGCAAG AACTGGACTC TTGGTATGTC
    ATTCTTAATG GGATTGTAGA AATCAGCCAT TCAGATGGCA GGATGGAAAA CCTTTGCATG
    GGAAATAGCT TTGGCATAAA CCCATCTTTA GAAACACAGT ACATGGATGG AGTAGTAAAA
    ACCAAAGCTG ATGATTGCCA GTTTGTGTGC ATAGCACAAC AGGACTACTT TCGGATATTA
    AACCATGTGG AAAAAAATAC CCACAAAGTC AAGGAAAAGG GGGAGATTGT TATGGTTAAT
    GAACACCGAG AACTGGATCG AAGTGGCACT CGAAAGGGAC ATATAGTAAT TCAGGGGACT
    CCAGAAAGAC TCATCTTGCA CCTAATAGAA GAGCACTCTG TTGTTGACCC AACATACATA
    GAGGATTTTC TATTAACATA CAGGACATTC CTTTCAAGTC CATTAGAGGT TGGGGACAAG
    CTTTTGGAGT GGTTTAAAGA TGACAGTCGT CGTGACAAGG TAACACGAGT TGTCCTTCTT
    TGGGTTAACA ACCACTTTAA TGATTTTGAA GGAGACCCTG CCATGACAAG GTTTTTGGAA
    GAATTTGAAC GGAATCTTGA AAATGCGAAA ATGAAGGGTC ATCTGCGACT GCTAAACATA
    GCCTGTGCTG CAAAGGCTAA ACTGAGACAG GTTACATTGC TTAAGCCTTC CCGTGAATCG
    CCTTTTTATT TCAACGTCGT TGGGGGAAGC GACAAAGGTT TCGGCATGTT TATTGAAAGT
    GTTGAGCCTG GAAGCAAAGC AACAGAAGCT GGATTAAAAC GCGGAGATCA GATTATGGAA
    GTCAATGGCC AAAATTTTGA GACTATCGTT TACGCCAAAG CCCTGGAGAT CTTGAAAAAC
    AATACACATC TTTCCATCAC TGTCAAGACC AACATTTTTG TGTTTAAAGA GCTTCTTAAT
    CGAATCGGGC AGGAGAAAAA AAATGGAGTC CCACACATCC CCAAAATTGC AGAGAAGAAG
    AACAATAGAT ATTCCATCCC TGATCTCCCC AATGATGTAG AACAGATGTT TCCACGGGAT
    CGCGCAAGCA AGAAAATGAA AGCCAATACG GTGTCTGGAG GGCGGAATCG CATCCGGAAG
    ATTTTGGACA AAACACGTTT TAGTATATTA CCACCTAAGC CATTCAGTGA TGGCCATGTT
    ATCCAGTCCC AGGATGACAG TATTGTTGGC ACCAGGCAGT GCAGGCACAG TGTGGCTATA
    ATGCCCATTC CGGGCACGCT CTCTTCAAGC AGTCCAGACT TGCTTCAAGC TACAACAAAC
    ATATTGGATT TCTCCAATCC TACTGATATT CCAGACCAAG TTATACGAAT ATTTAAAGCA
    GATCAGCAGT GCTGCTATAT TATCATCGGC AAAGACACTA CTGCAAAGGA GGTGGTGATA
    CAAGCACTTC ATGAATTCAG CCTTACTGGG TCAACAGACA CATACTCTCT CTGTGAGGTC
    TCGGTCAGTC CAGAAGGTGT CATTAAACAA AGACGGCTTC CGGATCAGCT TTCTAAACTG
    GCAGACAGAA TCCAGCTGAA TGGAAGGTAT TATTTGAAGA ACAACATGGA GACCGAAACG
    TTGTGTTCAG ATGAAGATGC TCAGGAGCTT CTGCGTGAAA GCCTCATCTC TCTCCTTCAG
    CTTAGCACTA TCGAGGTGGC AACACAGCTT TCTATGCGGG ACTTTGAACT CTTCCGCAAT
    ATTGAGCCCA CAGAATACAT AGATGACCTT TTCAAGATAG ATTCCAAGAC AAGTGCTGCT
    CATCTGAAAC AGTTTGAGGA AGTGATCAAC CAAGAGACAT TTTGGGTGGC CACGGAAATT
    CTAAGAGAAC CAAACCATTT GAAGAGAATG AAAATTGTGA AGCATTTCAT AAAGACTGCC
    CTCCATTGCA GAGAATGCAG AAATTTTAAC TCCATGTTTG CTATAATAAG TGGGCTCAAT
    CTTGCACCTG TGGCTCGGCT TCGAGGAACT TGGGAAAAGT TGCCCAGCAA ATACGAGAAG
    CTGCTTCGAG ACCTGCAAGA TCTCTTCGAC CCCTCAAGAA ATATGGCGAA ATACCGTAAT
    ATTCTAAGCA GTCAGAGCAT GCAGCCCCCC ATCATTCCTC TCTTCCCAGT CGTCAAGAAG
    GATATAACAT TTCTTCATGA AGGAAATGAT TCAAAAGTTG ATGGGTTAGT AAATTTTGAG
    AAGCTGCGAA TGATTGCAAA GGAGATTCGA CATGTTATTC GCATGACTTC TGCTAATATG
    GATCCAGCAG TTATGTTTAG GCAAAGGAAG AAGAGATGGA GAAGTTTAGG GTCCCTAAGC
    CAAGGCAGCA CAAACTCTAA CATGCTGGAT GTTCAAGGAG GTGGTCACAA GAAACGGGCT
    CGGCGAAGTT CATTCCTTAA TGCTAAGAAG CTGTATGAAG ATGCACAGAT GTCAAGGAAG
    GTAAAACAGT ACATCTCCAA CCTGAGTGTG GAAATCGATG AGGAGAAGAT CCAGCTAATG
    TCACTGCAAT GTGAACCAGC CTACAGCACT TTGGCAAAGA ATTTAAGTGA GAGAAGATCT
    GCAAAATCTG ACTTGTCTCC TGCACCTTTG AGGCCATCAG GCCAAACTAC CAAAGCTCAC
    ATCCATCAGC AAAACAGAAT GAGCCAAGTA TTGCAAGTCC CTACAGTTAA CTTATATCCA
    ATGCGAAAGA AAGCACCAAC CAAGGAACAT CTTCCATGCA CTTCAGGATC TTCACAGAAA
    ATATCTGGTT TGACAGATGA GGTTACAGTT AAAGCACAGT TAGAAGACTC TGTTTCTCTT
    GTGTCATCAT TGCATTCCAG CCCAGCCGCA TCCCCTCAAG GATCTCCACA TAAAGTTGTT
    AACATTTCAA GATCAGAGAA TTCCAGTCAT ATGAACCTCT CTGGATCCTC CTCATCATTA
    ACCAGTGATC CCAACCAGAA GATCTGCAGT CGGCGTGGCT ATGGAATAGG CTATGCCCAT
    AACCCCTCGG CCAAATCTGA TAACTTCTCA GACTCAAGCA ACAGTGAGAT CTCGTCCCGA
    TCAAGCCTCG TCAGCAACTG CTCAGTAGAC TCCATGTCGT TGACCCTGCA GGATGAAAAG
    TGTGTGCCTC ATTCACATGC TGAGTCAATG GGACTATCAG AAAGAAAAGA CGTCCATCCG
    TCCTCTGAGT ATAACCAGCC CTGCAGTAGC TGGCTCCATG CAAGGCACCC TGCAGTCCGG
    AATTTCATAG CTTGCGCAAG CAGTGAGGAC ATAGCTCATG ACCACATATC GGTAGAGCCG
    GTAGACAGTG GTCGGGGCAG TTGGACATCT TGTTCTAGCA GTTCACATGA CAATTTCCAG
    ACTTTGCAGA AGGGACTGGA CCGCTTAAGC ACTTACCGCC AATCACACAT TGATTCCCCA
    ATTGCTGAGA CAGAGTCCGC AAACTGCAGC ACAGATGAAA CATGTAGTGA AGACTGTACC
    AAAGGAAAAA GGCACTCAAA CAACAGTTTA GAACTAAATG AATCTCGGCA AAGTTGGGCT
    TCATCTAGCT CAATGTCAGA TACTTACGAG ACGAACTATG GCACAATCAA GCGGAGAGTA
    TTGGACACAG AGGAAAAAAC AGAGGCTTTG GGCCAACAAT TAGATGTCTT TTATAAAACA
    GTTACTTCCA CTACTGAAAA AGGACTGATA GTGTACTGTG TCACCTCATC CCAGAAGGAC
    AGTAGGTATA GGGAGCCACC TCCCACTCCT CCAGGCTACC AGGGGATATC ATTAGCCGAC
    ATAAAGGAAG GACAACCCCA CCAAGCGCAC TTAAAACCTC CAGATTATAG TGTGGCGATA
    CAGAGGTCAA AGATGATACA TAAAGACCTC TCTAGGCTTT CATCAGCCTG TGTTGAACCA
    GTCTCATGTG TCTCACAGAA GATGATAACC CATTGGCATA ACCGGCATCA GCCTTATCCT
    AATGTACAGG CTGACAGAGA TAGTAAAGAA GATGAAGATG AACAAATGTC AGCAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa43155
    Clone ID Related Accession (Same CDS sequence) XM_018092132.1 , XM_018092132.2
    Accession Version XM_018092132.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4911bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 6 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTGCCA GCATTGAACC CAGGTTTAAA GAAGCCTTGA GGAAAAAACC CAAGGAAAGG 
    ACTCAGGAGG ATCTGAAAAC TGTTTATTCA TATATGCATA GAATGGATAT ACTCTCACAT
    CTCAGAGAAC ATCAATTAAG ATTGATGTCT GCAACAGCAC GATATGAAAG ATACAGTGCA
    AATCAGGTTC TATTCTGCTC AGAAAGTTTG GCTAGATGTT GGTACATTCT TCTTTCTGGT
    TCAGTTCTGG TAAAAAACTC CATGTTTTTG CCTCCATGCA GTTTTGGCAA GCAGTCAGGT
    GGCAAAAGGG GTTGTGATTG CATTGTACTG GAACCTTCTG AAATGATTGT GGTGGAAAAT
    ACAGGAGAAA ACAATGATGG CATTCTGCAT AGGGAAACTT CTATTAGACA ATCCCGGCGT
    CGGTATCGAA AAATCAACCA GCATGGAGAA CGCCAAACAA TTACAGATAA TGGGGATGCT
    GCTAGTTATG TCTTGCTCCC CACAGACCTC TCCAAGATAC ACCTCACCGA CAGTCCCCAC
    CCACATGTTA CACATGTGCC ATCCAGCCAA TCAGGATGCA GCATTGCAAG CGACTCTGGA
    AGCAGCAGCT TATCAGATAT CTACCAGGCA ACAGAAAGTG AGATGGGGGA TGTTGACTTG
    ACAGGCCTGC CAGAGGCAGC TGTTGATTCT GAAGATGAAG AGGAAGATGA AGATATTGAG
    AGAGCTTCAG ACACAATGCT GACCCGTGAC ATTGTGAGGG AATGTCTTGA GAAAGAACCC
    ACAGACAGAA CAGATGATGA CATAGAACAG CTACTGGAAT TCATGCATCA GCTTCCAGCC
    TTTGTCAATA TGACCATGTC TGTGAGACGA AAGCTTTGCT CGGTCATGAT TTTTGAAGTT
    GTGGAACAGG CTGGCGCAAT CATCATTGAA GATGGGCAAG AACTGGACTC TTGGTATGTC
    ATTCTTAATG GGATTGTAGA AATCAGCCAT TCAGATGGCA GGATGGAAAA CCTTTGCATG
    GGAAATAGCT TTGGCATAAA CCCATCTTTA GAAACACAGT ACATGGATGG AGTAGTAAAA
    ACCAAAGCTG ATGATTGCCA GTTTGTGTGC ATAGCACAAC AGGACTACTT TCGGATATTA
    AACCATGTGG AAAAAAATAC CCACAAAGTC AAGGAAAAGG GGGAGATTGT TATGGTTAAT
    GAACACCGAG AACTGGATCG AAGTGGCACT CGAAAGGGAC ATATAGTAAT TCAGGGGACT
    CCAGAAAGAC TCATCTTGCA CCTAATAGAA GAGCACTCTG TTGTTGACCC AACATACATA
    GAGGATTTTC TATTAACATA CAGGACATTC CTTTCAAGTC CATTAGAGGT TGGGGACAAG
    CTTTTGGAGT GGTTTAAAGA TGACAGTCGT CGTGACAAGG TAACACGAGT TGTCCTTCTT
    TGGGTTAACA ACCACTTTAA TGATTTTGAA GGAGACCCTG CCATGACAAG GTTTTTGGAA
    GAATTTGAAC GGAATCTTGA AAATGCGAAA ATGAAGGGTC ATCTGCGACT GCTAAACATA
    GCCTGTGCTG CAAAGGCTAA ACTGAGACAG GTTACATTGC TTAAGCCTTC CCGTGAATCG
    CCTTTTTATT TCAACGTCGT TGGGGGAAGC GACAAAGGTT TCGGCATGTT TATTGAAAGT
    GTTGAGCCTG GAAGCAAAGC AACAGAAGCT GGATTAAAAC GCGGAGATCA GATTATGGAA
    GTCAATGGCC AAAATTTTGA GACTATCGTT TACGCCAAAG CCCTGGAGAT CTTGAAAAAC
    AATACACATC TTTCCATCAC TGTCAAGACC AACATTTTTG TGTTTAAAGA GCTTCTTAAT
    CGAATCGGGC AGGAGAAAAA AAATGGAGTC CCACACATCC CCAAAATTGC AGAGAAGAAG
    AACAATAGAT ATTCCATCCC TGATCTCCCC AATGATGTAG AACAGATGTT TCCACGGGAT
    CGCGCAAGCA AGAAAATGAA AGCCAATACG GTGTCTGGAG GGCGGAATCG CATCCGGAAG
    ATTTTGGACA AAACACGTTT TAGTATATTA CCACCTAAGC CATTCAGTGA TGGCCATGTT
    ATCCAGTCCC AGGATGACAG TATTGTTGGC ACCAGGCAGT GCAGGCACAG TGTGGCTATA
    ATGCCCATTC CGGGCACGCT CTCTTCAAGC AGTCCAGACT TGCTTCAAGC TACAACAAAC
    ATATTGGATT TCTCCAATCC TACTGCTTTT GGATTTTACT ATATTCCAGA CCAAGTTATA
    CGAATATTTA AAGCAGATCA GCAGTGCTGC TATATTATCA TCGGCAAAGA CACTACTGCA
    AAGGAGGTGG TGATACAAGC ACTTCATGAA TTCAGCCTTA CTGGGTCAAC AGACACATAC
    TCTCTCTGTG AGGTCTCGGT CAGTCCAGAA GGTGTCATTA AACAAAGACG GCTTCCGGAT
    CAGCTTTCTA AACTGGCAGA CAGAATCCAG CTGAATGGAA GGTATTATTT GAAGAACAAC
    ATGGAGACCG AAACGTTGTG TTCAGATGAA GATGCTCAGG AGCTTCTGCG TGAAAGCCTC
    ATCTCTCTCC TTCAGCTTAG CACTATCGAG GTGGCAACAC AGCTTTCTAT GCGGGACTTT
    GAACTCTTCC GCAATATTGA GCCCACAGAA TACATAGATG ACCTTTTCAA GATAGATTCC
    AAGACAAGTG CTGCTCATCT GAAACAGTTT GAGGAAGTGA TCAACCAAGA GACATTTTGG
    GTGGCCACGG AAATTCTAAG AGAACCAAAC CATTTGAAGA GAATGAAAAT TGTGAAGCAT
    TTCATAAAGA CTGCCCTCCA TTGCAGAGAA TGCAGAAATT TTAACTCCAT GTTTGCTATA
    ATAAGTGGGC TCAATCTTGC ACCTGTGGCT CGGCTTCGAG GAACTTGGGA AAAGTTGCCC
    AGCAAATACG AGAAGCTGCT TCGAGACCTG CAAGATCTCT TCGACCCCTC AAGAAATATG
    GCGAAATACC GTAATATTCT AAGCAGTCAG AGCATGCAGC CCCCCATCAT TCCTCTCTTC
    CCAGTCGTCA AGAAGGATAT AACATTTCTT CATGAAGGAA ATGATTCAAA AGTTGATGGG
    TTAGTAAATT TTGAGAAGCT GCGAATGATT GCAAAGGAGA TTCGACATGT TATTCGCATG
    ACTTCTGCTA ATATGGATCC AGCAGTTATG TTTAGGCAAA GGTCCCTAAG CCAAGGCAGC
    ACAAACTCTA ACATGCTGGA TGTTCAAGGA GGTGGTCACA AGAAACGGGC TCGGCGAAGT
    TCATTCCTTA ATGCTAAGAA GCTGTATGAA GATGCACAGA TGTCAAGGAA GGTAAAACAG
    TACATCTCCA ACCTGAGTGT GGAAATCGAT GAGGAGAAGA TCCAGCTAAT GTCACTGCAA
    TGTGAACCAG CCTACAGCAC TTTGGCAAAG AATTTAAGTG AGAGAAGATC TGCAAAATCT
    GACTTGTCTC CTGCACCTTT GAGGCCATCA GGCCAAACTA CCAAAGCTCA CATCCATCAG
    CAAAACAGAA TGAGCCAAGT ATTGCAAGTC CCTACAGTTA ACTTATATCC AATGCGAAAG
    AAAGCACCAA CCAAGGAACA TCTTCCATGC ACTTCAGGAT CTTCACAGAA AATATCTGGT
    TTGACAGATG AGGTTACAGT TAAAGCACAG TTAGAAGACT CTGTTTCTCT TGTGTCATCA
    TTGCATTCCA GCCCAGCCGC ATCCCCTCAA GGATCTCCAC ATAAAGTTGT TAACATTTCA
    AGATCAGAGA ATTCCAGTCA TATGAACCTC TCTGGATCCT CCTCATCATT AACCAGTGAT
    CCCAACCAGA AGATCTGCAG TCGGCGTGGC TATGGAATAG GCTATGCCCA TAACCCCTCG
    GCCAAATCTG ATAACTTCTC AGACTCAAGC AACAGTGAGA TCTCGTCCCG ATCAAGCCTC
    GTCAGCAACT GCTCAGTAGA CTCCATGTCG TTGACCCTGC AGGATGAAAA GTGTGTGCCT
    CATTCACATG CTGAGTCAAT GGGACTATCA GAAAGAAAAG ACGTCCATCC GTCCTCTGAG
    TATAACCAGC CCTGCAGTAG CTGGCTCCAT GCAAGGCACC CTGCAGTCCG GAATTTCATA
    GCTTGCGCAA GCAGTGAGGA CATAGCTCAT GACCACATAT CGGTAGAGCC GGTAGACAGT
    GGTCGGGGCA GTTGGACATC TTGTTCTAGC AGTTCACATG ACAATTTCCA GACTTTGCAG
    AAGGGACTGG ACCGCTTAAG CACTTACCGC CAATCACACA TTGATTCCCC AATTGCTGAG
    ACAGAGTCCG CAAACTGCAG CACAGATGAA ACATGTAGTG AAGACTGTAC CAAAGGAAAA
    AGGCACTCAA ACAACAGTTT AGAACTAAAT GAATCTCGGC AAAGTTGGGC TTCATCTAGC
    TCAATGTCAG ATACTTACGA GACGAACTAT GGCACAATCA AGCGGAGAGT ATTGGACACA
    GAGGAAAAAA CAGAGGCTTT GGGCCAACAA TTAGATGTCT TTTATAAAAC AGTTACTTCC
    ACTACTGAAA AAGGACTGAT AGTGTACTGT GTCACCTCAT CCCAGAAGGA CAGTAGGTAT
    AGGGAGCCAC CTCCCACTCC TCCAGGCTAC CAGGGGATAT CATTAGCCGA CATAAAGGAA
    GGACAACCCC ACCAAGCGCA CTTAAAACCT CCAGATTATA GTGTGGCGAT ACAGAGGTCA
    AAGATGATAC ATAAAGACCT CTCTAGGCTT TCATCAGCCT GTGTTGAACC AGTCTCATGT
    GTCTCACAGA AGATGATAAC CCATTGGCAT AACCGGCATC AGCCTTATCC TAATGTACAG
    GCTGACAGAG ATAGTAAAGA AGATGAAGAT GAACAAATGT CAGCAGTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947621.1
    CDS1356..6266
    Translation

    Target ORF information:

    RefSeq Version XM_018092132.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 6 (rapgef6), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092132.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTGCCA GCATTGAACC CAGGTTTAAA GAAGCCTTGA GGAAAAAACC CAAGGAAAGG 
    ACTCAGGAGG ATCTGAAAAC TGTTTATTCA TATATGCATA GAATGGATAT ACTCTCACAT
    CTCAGAGAAC ATCAATTAAG ATTGATGTCT GCAACAGCAC GATATGAAAG ATACAGTGCA
    AATCAGGTTC TATTCTGCTC AGAAAGTTTG GCTAGATGTT GGTACATTCT TCTTTCTGGT
    TCAGTTCTGG TAAAAAACTC CATGTTTTTG CCTCCATGCA GTTTTGGCAA GCAGTCAGGT
    GGCAAAAGGG GTTGTGATTG CATTGTACTG GAACCTTCTG AAATGATTGT GGTGGAAAAT
    ACAGGAGAAA ACAATGATGG CATTCTGCAT AGGGAAACTT CTATTAGACA ATCCCGGCGT
    CGGTATCGAA AAATCAACCA GCATGGAGAA CGCCAAACAA TTACAGATAA TGGGGATGCT
    GCTAGTTATG TCTTGCTCCC CACAGACCTC TCCAAGATAC ACCTCACCGA CAGTCCCCAC
    CCACATGTTA CACATGTGCC ATCCAGCCAA TCAGGATGCA GCATTGCAAG CGACTCTGGA
    AGCAGCAGCT TATCAGATAT CTACCAGGCA ACAGAAAGTG AGATGGGGGA TGTTGACTTG
    ACAGGCCTGC CAGAGGCAGC TGTTGATTCT GAAGATGAAG AGGAAGATGA AGATATTGAG
    AGAGCTTCAG ACACAATGCT GACCCGTGAC ATTGTGAGGG AATGTCTTGA GAAAGAACCC
    ACAGACAGAA CAGATGATGA CATAGAACAG CTACTGGAAT TCATGCATCA GCTTCCAGCC
    TTTGTCAATA TGACCATGTC TGTGAGACGA AAGCTTTGCT CGGTCATGAT TTTTGAAGTT
    GTGGAACAGG CTGGCGCAAT CATCATTGAA GATGGGCAAG AACTGGACTC TTGGTATGTC
    ATTCTTAATG GGATTGTAGA AATCAGCCAT TCAGATGGCA GGATGGAAAA CCTTTGCATG
    GGAAATAGCT TTGGCATAAA CCCATCTTTA GAAACACAGT ACATGGATGG AGTAGTAAAA
    ACCAAAGCTG ATGATTGCCA GTTTGTGTGC ATAGCACAAC AGGACTACTT TCGGATATTA
    AACCATGTGG AAAAAAATAC CCACAAAGTC AAGGAAAAGG GGGAGATTGT TATGGTTAAT
    GAACACCGAG AACTGGATCG AAGTGGCACT CGAAAGGGAC ATATAGTAAT TCAGGGGACT
    CCAGAAAGAC TCATCTTGCA CCTAATAGAA GAGCACTCTG TTGTTGACCC AACATACATA
    GAGGATTTTC TATTAACATA CAGGACATTC CTTTCAAGTC CATTAGAGGT TGGGGACAAG
    CTTTTGGAGT GGTTTAAAGA TGACAGTCGT CGTGACAAGG TAACACGAGT TGTCCTTCTT
    TGGGTTAACA ACCACTTTAA TGATTTTGAA GGAGACCCTG CCATGACAAG GTTTTTGGAA
    GAATTTGAAC GGAATCTTGA AAATGCGAAA ATGAAGGGTC ATCTGCGACT GCTAAACATA
    GCCTGTGCTG CAAAGGCTAA ACTGAGACAG GTTACATTGC TTAAGCCTTC CCGTGAATCG
    CCTTTTTATT TCAACGTCGT TGGGGGAAGC GACAAAGGTT TCGGCATGTT TATTGAAAGT
    GTTGAGCCTG GAAGCAAAGC AACAGAAGCT GGATTAAAAC GCGGAGATCA GATTATGGAA
    GTCAATGGCC AAAATTTTGA GACTATCGTT TACGCCAAAG CCCTGGAGAT CTTGAAAAAC
    AATACACATC TTTCCATCAC TGTCAAGACC AACATTTTTG TGTTTAAAGA GCTTCTTAAT
    CGAATCGGGC AGGAGAAAAA AAATGGAGTC CCACACATCC CCAAAATTGC AGAGAAGAAG
    AACAATAGAT ATTCCATCCC TGATCTCCCC AATGATGTAG AACAGATGTT TCCACGGGAT
    CGCGCAAGCA AGAAAATGAA AGCCAATACG GTGTCTGGAG GGCGGAATCG CATCCGGAAG
    ATTTTGGACA AAACACGTTT TAGTATATTA CCACCTAAGC CATTCAGTGA TGGCCATGTT
    ATCCAGTCCC AGGATGACAG TATTGTTGGC ACCAGGCAGT GCAGGCACAG TGTGGCTATA
    ATGCCCATTC CGGGCACGCT CTCTTCAAGC AGTCCAGACT TGCTTCAAGC TACAACAAAC
    ATATTGGATT TCTCCAATCC TACTGCTTTT GGATTTTACT ATATTCCAGA CCAAGTTATA
    CGAATATTTA AAGCAGATCA GCAGTGCTGC TATATTATCA TCGGCAAAGA CACTACTGCA
    AAGGAGGTGG TGATACAAGC ACTTCATGAA TTCAGCCTTA CTGGGTCAAC AGACACATAC
    TCTCTCTGTG AGGTCTCGGT CAGTCCAGAA GGTGTCATTA AACAAAGACG GCTTCCGGAT
    CAGCTTTCTA AACTGGCAGA CAGAATCCAG CTGAATGGAA GGTATTATTT GAAGAACAAC
    ATGGAGACCG AAACGTTGTG TTCAGATGAA GATGCTCAGG AGCTTCTGCG TGAAAGCCTC
    ATCTCTCTCC TTCAGCTTAG CACTATCGAG GTGGCAACAC AGCTTTCTAT GCGGGACTTT
    GAACTCTTCC GCAATATTGA GCCCACAGAA TACATAGATG ACCTTTTCAA GATAGATTCC
    AAGACAAGTG CTGCTCATCT GAAACAGTTT GAGGAAGTGA TCAACCAAGA GACATTTTGG
    GTGGCCACGG AAATTCTAAG AGAACCAAAC CATTTGAAGA GAATGAAAAT TGTGAAGCAT
    TTCATAAAGA CTGCCCTCCA TTGCAGAGAA TGCAGAAATT TTAACTCCAT GTTTGCTATA
    ATAAGTGGGC TCAATCTTGC ACCTGTGGCT CGGCTTCGAG GAACTTGGGA AAAGTTGCCC
    AGCAAATACG AGAAGCTGCT TCGAGACCTG CAAGATCTCT TCGACCCCTC AAGAAATATG
    GCGAAATACC GTAATATTCT AAGCAGTCAG AGCATGCAGC CCCCCATCAT TCCTCTCTTC
    CCAGTCGTCA AGAAGGATAT AACATTTCTT CATGAAGGAA ATGATTCAAA AGTTGATGGG
    TTAGTAAATT TTGAGAAGCT GCGAATGATT GCAAAGGAGA TTCGACATGT TATTCGCATG
    ACTTCTGCTA ATATGGATCC AGCAGTTATG TTTAGGCAAA GGTCCCTAAG CCAAGGCAGC
    ACAAACTCTA ACATGCTGGA TGTTCAAGGA GGTGGTCACA AGAAACGGGC TCGGCGAAGT
    TCATTCCTTA ATGCTAAGAA GCTGTATGAA GATGCACAGA TGTCAAGGAA GGTAAAACAG
    TACATCTCCA ACCTGAGTGT GGAAATCGAT GAGGAGAAGA TCCAGCTAAT GTCACTGCAA
    TGTGAACCAG CCTACAGCAC TTTGGCAAAG AATTTAAGTG AGAGAAGATC TGCAAAATCT
    GACTTGTCTC CTGCACCTTT GAGGCCATCA GGCCAAACTA CCAAAGCTCA CATCCATCAG
    CAAAACAGAA TGAGCCAAGT ATTGCAAGTC CCTACAGTTA ACTTATATCC AATGCGAAAG
    AAAGCACCAA CCAAGGAACA TCTTCCATGC ACTTCAGGAT CTTCACAGAA AATATCTGGT
    TTGACAGATG AGGTTACAGT TAAAGCACAG TTAGAAGACT CTGTTTCTCT TGTGTCATCA
    TTGCATTCCA GCCCAGCCGC ATCCCCTCAA GGATCTCCAC ATAAAGTTGT TAACATTTCA
    AGATCAGAGA ATTCCAGTCA TATGAACCTC TCTGGATCCT CCTCATCATT AACCAGTGAT
    CCCAACCAGA AGATCTGCAG TCGGCGTGGC TATGGAATAG GCTATGCCCA TAACCCCTCG
    GCCAAATCTG ATAACTTCTC AGACTCAAGC AACAGTGAGA TCTCGTCCCG ATCAAGCCTC
    GTCAGCAACT GCTCAGTAGA CTCCATGTCG TTGACCCTGC AGGATGAAAA GTGTGTGCCT
    CATTCACATG CTGAGTCAAT GGGACTATCA GAAAGAAAAG ACGTCCATCC GTCCTCTGAG
    TATAACCAGC CCTGCAGTAG CTGGCTCCAT GCAAGGCACC CTGCAGTCCG GAATTTCATA
    GCTTGCGCAA GCAGTGAGGA CATAGCTCAT GACCACATAT CGGTAGAGCC GGTAGACAGT
    GGTCGGGGCA GTTGGACATC TTGTTCTAGC AGTTCACATG ACAATTTCCA GACTTTGCAG
    AAGGGACTGG ACCGCTTAAG CACTTACCGC CAATCACACA TTGATTCCCC AATTGCTGAG
    ACAGAGTCCG CAAACTGCAG CACAGATGAA ACATGTAGTG AAGACTGTAC CAAAGGAAAA
    AGGCACTCAA ACAACAGTTT AGAACTAAAT GAATCTCGGC AAAGTTGGGC TTCATCTAGC
    TCAATGTCAG ATACTTACGA GACGAACTAT GGCACAATCA AGCGGAGAGT ATTGGACACA
    GAGGAAAAAA CAGAGGCTTT GGGCCAACAA TTAGATGTCT TTTATAAAAC AGTTACTTCC
    ACTACTGAAA AAGGACTGAT AGTGTACTGT GTCACCTCAT CCCAGAAGGA CAGTAGGTAT
    AGGGAGCCAC CTCCCACTCC TCCAGGCTAC CAGGGGATAT CATTAGCCGA CATAAAGGAA
    GGACAACCCC ACCAAGCGCA CTTAAAACCT CCAGATTATA GTGTGGCGAT ACAGAGGTCA
    AAGATGATAC ATAAAGACCT CTCTAGGCTT TCATCAGCCT GTGTTGAACC AGTCTCATGT
    GTCTCACAGA AGATGATAAC CCATTGGCAT AACCGGCATC AGCCTTATCC TAATGTACAG
    GCTGACAGAG ATAGTAAAGA AGATGAAGAT GAACAAATGT CAGCAGTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa43155
    Clone ID Related Accession (Same CDS sequence) XM_018092132.1 , XM_018092132.2
    Accession Version XM_018092132.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4911bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 6 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018092132.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTGCCA GCATTGAACC CAGGTTTAAA GAAGCCTTGA GGAAAAAACC CAAGGAAAGG 
    ACTCAGGAGG ATCTGAAAAC TGTTTATTCA TATATGCATA GAATGGATAT ACTCTCACAT
    CTCAGAGAAC ATCAATTAAG ATTGATGTCT GCAACAGCAC GATATGAAAG ATACAGTGCA
    AATCAGGTTC TATTCTGCTC AGAAAGTTTG GCTAGATGTT GGTACATTCT TCTTTCTGGT
    TCAGTTCTGG TAAAAAACTC CATGTTTTTG CCTCCATGCA GTTTTGGCAA GCAGTCAGGT
    GGCAAAAGGG GTTGTGATTG CATTGTACTG GAACCTTCTG AAATGATTGT GGTGGAAAAT
    ACAGGAGAAA ACAATGATGG CATTCTGCAT AGGGAAACTT CTATTAGACA ATCCCGGCGT
    CGGTATCGAA AAATCAACCA GCATGGAGAA CGCCAAACAA TTACAGATAA TGGGGATGCT
    GCTAGTTATG TCTTGCTCCC CACAGACCTC TCCAAGATAC ACCTCACCGA CAGTCCCCAC
    CCACATGTTA CACATGTGCC ATCCAGCCAA TCAGGATGCA GCATTGCAAG CGACTCTGGA
    AGCAGCAGCT TATCAGATAT CTACCAGGCA ACAGAAAGTG AGATGGGGGA TGTTGACTTG
    ACAGGCCTGC CAGAGGCAGC TGTTGATTCT GAAGATGAAG AGGAAGATGA AGATATTGAG
    AGAGCTTCAG ACACAATGCT GACCCGTGAC ATTGTGAGGG AATGTCTTGA GAAAGAACCC
    ACAGACAGAA CAGATGATGA CATAGAACAG CTACTGGAAT TCATGCATCA GCTTCCAGCC
    TTTGTCAATA TGACCATGTC TGTGAGACGA AAGCTTTGCT CGGTCATGAT TTTTGAAGTT
    GTGGAACAGG CTGGCGCAAT CATCATTGAA GATGGGCAAG AACTGGACTC TTGGTATGTC
    ATTCTTAATG GGATTGTAGA AATCAGCCAT TCAGATGGCA GGATGGAAAA CCTTTGCATG
    GGAAATAGCT TTGGCATAAA CCCATCTTTA GAAACACAGT ACATGGATGG AGTAGTAAAA
    ACCAAAGCTG ATGATTGCCA GTTTGTGTGC ATAGCACAAC AGGACTACTT TCGGATATTA
    AACCATGTGG AAAAAAATAC CCACAAAGTC AAGGAAAAGG GGGAGATTGT TATGGTTAAT
    GAACACCGAG AACTGGATCG AAGTGGCACT CGAAAGGGAC ATATAGTAAT TCAGGGGACT
    CCAGAAAGAC TCATCTTGCA CCTAATAGAA GAGCACTCTG TTGTTGACCC AACATACATA
    GAGGATTTTC TATTAACATA CAGGACATTC CTTTCAAGTC CATTAGAGGT TGGGGACAAG
    CTTTTGGAGT GGTTTAAAGA TGACAGTCGT CGTGACAAGG TAACACGAGT TGTCCTTCTT
    TGGGTTAACA ACCACTTTAA TGATTTTGAA GGAGACCCTG CCATGACAAG GTTTTTGGAA
    GAATTTGAAC GGAATCTTGA AAATGCGAAA ATGAAGGGTC ATCTGCGACT GCTAAACATA
    GCCTGTGCTG CAAAGGCTAA ACTGAGACAG GTTACATTGC TTAAGCCTTC CCGTGAATCG
    CCTTTTTATT TCAACGTCGT TGGGGGAAGC GACAAAGGTT TCGGCATGTT TATTGAAAGT
    GTTGAGCCTG GAAGCAAAGC AACAGAAGCT GGATTAAAAC GCGGAGATCA GATTATGGAA
    GTCAATGGCC AAAATTTTGA GACTATCGTT TACGCCAAAG CCCTGGAGAT CTTGAAAAAC
    AATACACATC TTTCCATCAC TGTCAAGACC AACATTTTTG TGTTTAAAGA GCTTCTTAAT
    CGAATCGGGC AGGAGAAAAA AAATGGAGTC CCACACATCC CCAAAATTGC AGAGAAGAAG
    AACAATAGAT ATTCCATCCC TGATCTCCCC AATGATGTAG AACAGATGTT TCCACGGGAT
    CGCGCAAGCA AGAAAATGAA AGCCAATACG GTGTCTGGAG GGCGGAATCG CATCCGGAAG
    ATTTTGGACA AAACACGTTT TAGTATATTA CCACCTAAGC CATTCAGTGA TGGCCATGTT
    ATCCAGTCCC AGGATGACAG TATTGTTGGC ACCAGGCAGT GCAGGCACAG TGTGGCTATA
    ATGCCCATTC CGGGCACGCT CTCTTCAAGC AGTCCAGACT TGCTTCAAGC TACAACAAAC
    ATATTGGATT TCTCCAATCC TACTGCTTTT GGATTTTACT ATATTCCAGA CCAAGTTATA
    CGAATATTTA AAGCAGATCA GCAGTGCTGC TATATTATCA TCGGCAAAGA CACTACTGCA
    AAGGAGGTGG TGATACAAGC ACTTCATGAA TTCAGCCTTA CTGGGTCAAC AGACACATAC
    TCTCTCTGTG AGGTCTCGGT CAGTCCAGAA GGTGTCATTA AACAAAGACG GCTTCCGGAT
    CAGCTTTCTA AACTGGCAGA CAGAATCCAG CTGAATGGAA GGTATTATTT GAAGAACAAC
    ATGGAGACCG AAACGTTGTG TTCAGATGAA GATGCTCAGG AGCTTCTGCG TGAAAGCCTC
    ATCTCTCTCC TTCAGCTTAG CACTATCGAG GTGGCAACAC AGCTTTCTAT GCGGGACTTT
    GAACTCTTCC GCAATATTGA GCCCACAGAA TACATAGATG ACCTTTTCAA GATAGATTCC
    AAGACAAGTG CTGCTCATCT GAAACAGTTT GAGGAAGTGA TCAACCAAGA GACATTTTGG
    GTGGCCACGG AAATTCTAAG AGAACCAAAC CATTTGAAGA GAATGAAAAT TGTGAAGCAT
    TTCATAAAGA CTGCCCTCCA TTGCAGAGAA TGCAGAAATT TTAACTCCAT GTTTGCTATA
    ATAAGTGGGC TCAATCTTGC ACCTGTGGCT CGGCTTCGAG GAACTTGGGA AAAGTTGCCC
    AGCAAATACG AGAAGCTGCT TCGAGACCTG CAAGATCTCT TCGACCCCTC AAGAAATATG
    GCGAAATACC GTAATATTCT AAGCAGTCAG AGCATGCAGC CCCCCATCAT TCCTCTCTTC
    CCAGTCGTCA AGAAGGATAT AACATTTCTT CATGAAGGAA ATGATTCAAA AGTTGATGGG
    TTAGTAAATT TTGAGAAGCT GCGAATGATT GCAAAGGAGA TTCGACATGT TATTCGCATG
    ACTTCTGCTA ATATGGATCC AGCAGTTATG TTTAGGCAAA GGTCCCTAAG CCAAGGCAGC
    ACAAACTCTA ACATGCTGGA TGTTCAAGGA GGTGGTCACA AGAAACGGGC TCGGCGAAGT
    TCATTCCTTA ATGCTAAGAA GCTGTATGAA GATGCACAGA TGTCAAGGAA GGTAAAACAG
    TACATCTCCA ACCTGAGTGT GGAAATCGAT GAGGAGAAGA TCCAGCTAAT GTCACTGCAA
    TGTGAACCAG CCTACAGCAC TTTGGCAAAG AATTTAAGTG AGAGAAGATC TGCAAAATCT
    GACTTGTCTC CTGCACCTTT GAGGCCATCA GGCCAAACTA CCAAAGCTCA CATCCATCAG
    CAAAACAGAA TGAGCCAAGT ATTGCAAGTC CCTACAGTTA ACTTATATCC AATGCGAAAG
    AAAGCACCAA CCAAGGAACA TCTTCCATGC ACTTCAGGAT CTTCACAGAA AATATCTGGT
    TTGACAGATG AGGTTACAGT TAAAGCACAG TTAGAAGACT CTGTTTCTCT TGTGTCATCA
    TTGCATTCCA GCCCAGCCGC ATCCCCTCAA GGATCTCCAC ATAAAGTTGT TAACATTTCA
    AGATCAGAGA ATTCCAGTCA TATGAACCTC TCTGGATCCT CCTCATCATT AACCAGTGAT
    CCCAACCAGA AGATCTGCAG TCGGCGTGGC TATGGAATAG GCTATGCCCA TAACCCCTCG
    GCCAAATCTG ATAACTTCTC AGACTCAAGC AACAGTGAGA TCTCGTCCCG ATCAAGCCTC
    GTCAGCAACT GCTCAGTAGA CTCCATGTCG TTGACCCTGC AGGATGAAAA GTGTGTGCCT
    CATTCACATG CTGAGTCAAT GGGACTATCA GAAAGAAAAG ACGTCCATCC GTCCTCTGAG
    TATAACCAGC CCTGCAGTAG CTGGCTCCAT GCAAGGCACC CTGCAGTCCG GAATTTCATA
    GCTTGCGCAA GCAGTGAGGA CATAGCTCAT GACCACATAT CGGTAGAGCC GGTAGACAGT
    GGTCGGGGCA GTTGGACATC TTGTTCTAGC AGTTCACATG ACAATTTCCA GACTTTGCAG
    AAGGGACTGG ACCGCTTAAG CACTTACCGC CAATCACACA TTGATTCCCC AATTGCTGAG
    ACAGAGTCCG CAAACTGCAG CACAGATGAA ACATGTAGTG AAGACTGTAC CAAAGGAAAA
    AGGCACTCAA ACAACAGTTT AGAACTAAAT GAATCTCGGC AAAGTTGGGC TTCATCTAGC
    TCAATGTCAG ATACTTACGA GACGAACTAT GGCACAATCA AGCGGAGAGT ATTGGACACA
    GAGGAAAAAA CAGAGGCTTT GGGCCAACAA TTAGATGTCT TTTATAAAAC AGTTACTTCC
    ACTACTGAAA AAGGACTGAT AGTGTACTGT GTCACCTCAT CCCAGAAGGA CAGTAGGTAT
    AGGGAGCCAC CTCCCACTCC TCCAGGCTAC CAGGGGATAT CATTAGCCGA CATAAAGGAA
    GGACAACCCC ACCAAGCGCA CTTAAAACCT CCAGATTATA GTGTGGCGAT ACAGAGGTCA
    AAGATGATAC ATAAAGACCT CTCTAGGCTT TCATCAGCCT GTGTTGAACC AGTCTCATGT
    GTCTCACAGA AGATGATAAC CCATTGGCAT AACCGGCATC AGCCTTATCC TAATGTACAG
    GCTGACAGAG ATAGTAAAGA AGATGAAGAT GAACAAATGT CAGCAGTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947621.1
    CDS485..5395
    Translation

    Target ORF information:

    RefSeq Version XM_018092132.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 6 (rapgef6), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092132.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTGCCA GCATTGAACC CAGGTTTAAA GAAGCCTTGA GGAAAAAACC CAAGGAAAGG 
    ACTCAGGAGG ATCTGAAAAC TGTTTATTCA TATATGCATA GAATGGATAT ACTCTCACAT
    CTCAGAGAAC ATCAATTAAG ATTGATGTCT GCAACAGCAC GATATGAAAG ATACAGTGCA
    AATCAGGTTC TATTCTGCTC AGAAAGTTTG GCTAGATGTT GGTACATTCT TCTTTCTGGT
    TCAGTTCTGG TAAAAAACTC CATGTTTTTG CCTCCATGCA GTTTTGGCAA GCAGTCAGGT
    GGCAAAAGGG GTTGTGATTG CATTGTACTG GAACCTTCTG AAATGATTGT GGTGGAAAAT
    ACAGGAGAAA ACAATGATGG CATTCTGCAT AGGGAAACTT CTATTAGACA ATCCCGGCGT
    CGGTATCGAA AAATCAACCA GCATGGAGAA CGCCAAACAA TTACAGATAA TGGGGATGCT
    GCTAGTTATG TCTTGCTCCC CACAGACCTC TCCAAGATAC ACCTCACCGA CAGTCCCCAC
    CCACATGTTA CACATGTGCC ATCCAGCCAA TCAGGATGCA GCATTGCAAG CGACTCTGGA
    AGCAGCAGCT TATCAGATAT CTACCAGGCA ACAGAAAGTG AGATGGGGGA TGTTGACTTG
    ACAGGCCTGC CAGAGGCAGC TGTTGATTCT GAAGATGAAG AGGAAGATGA AGATATTGAG
    AGAGCTTCAG ACACAATGCT GACCCGTGAC ATTGTGAGGG AATGTCTTGA GAAAGAACCC
    ACAGACAGAA CAGATGATGA CATAGAACAG CTACTGGAAT TCATGCATCA GCTTCCAGCC
    TTTGTCAATA TGACCATGTC TGTGAGACGA AAGCTTTGCT CGGTCATGAT TTTTGAAGTT
    GTGGAACAGG CTGGCGCAAT CATCATTGAA GATGGGCAAG AACTGGACTC TTGGTATGTC
    ATTCTTAATG GGATTGTAGA AATCAGCCAT TCAGATGGCA GGATGGAAAA CCTTTGCATG
    GGAAATAGCT TTGGCATAAA CCCATCTTTA GAAACACAGT ACATGGATGG AGTAGTAAAA
    ACCAAAGCTG ATGATTGCCA GTTTGTGTGC ATAGCACAAC AGGACTACTT TCGGATATTA
    AACCATGTGG AAAAAAATAC CCACAAAGTC AAGGAAAAGG GGGAGATTGT TATGGTTAAT
    GAACACCGAG AACTGGATCG AAGTGGCACT CGAAAGGGAC ATATAGTAAT TCAGGGGACT
    CCAGAAAGAC TCATCTTGCA CCTAATAGAA GAGCACTCTG TTGTTGACCC AACATACATA
    GAGGATTTTC TATTAACATA CAGGACATTC CTTTCAAGTC CATTAGAGGT TGGGGACAAG
    CTTTTGGAGT GGTTTAAAGA TGACAGTCGT CGTGACAAGG TAACACGAGT TGTCCTTCTT
    TGGGTTAACA ACCACTTTAA TGATTTTGAA GGAGACCCTG CCATGACAAG GTTTTTGGAA
    GAATTTGAAC GGAATCTTGA AAATGCGAAA ATGAAGGGTC ATCTGCGACT GCTAAACATA
    GCCTGTGCTG CAAAGGCTAA ACTGAGACAG GTTACATTGC TTAAGCCTTC CCGTGAATCG
    CCTTTTTATT TCAACGTCGT TGGGGGAAGC GACAAAGGTT TCGGCATGTT TATTGAAAGT
    GTTGAGCCTG GAAGCAAAGC AACAGAAGCT GGATTAAAAC GCGGAGATCA GATTATGGAA
    GTCAATGGCC AAAATTTTGA GACTATCGTT TACGCCAAAG CCCTGGAGAT CTTGAAAAAC
    AATACACATC TTTCCATCAC TGTCAAGACC AACATTTTTG TGTTTAAAGA GCTTCTTAAT
    CGAATCGGGC AGGAGAAAAA AAATGGAGTC CCACACATCC CCAAAATTGC AGAGAAGAAG
    AACAATAGAT ATTCCATCCC TGATCTCCCC AATGATGTAG AACAGATGTT TCCACGGGAT
    CGCGCAAGCA AGAAAATGAA AGCCAATACG GTGTCTGGAG GGCGGAATCG CATCCGGAAG
    ATTTTGGACA AAACACGTTT TAGTATATTA CCACCTAAGC CATTCAGTGA TGGCCATGTT
    ATCCAGTCCC AGGATGACAG TATTGTTGGC ACCAGGCAGT GCAGGCACAG TGTGGCTATA
    ATGCCCATTC CGGGCACGCT CTCTTCAAGC AGTCCAGACT TGCTTCAAGC TACAACAAAC
    ATATTGGATT TCTCCAATCC TACTGCTTTT GGATTTTACT ATATTCCAGA CCAAGTTATA
    CGAATATTTA AAGCAGATCA GCAGTGCTGC TATATTATCA TCGGCAAAGA CACTACTGCA
    AAGGAGGTGG TGATACAAGC ACTTCATGAA TTCAGCCTTA CTGGGTCAAC AGACACATAC
    TCTCTCTGTG AGGTCTCGGT CAGTCCAGAA GGTGTCATTA AACAAAGACG GCTTCCGGAT
    CAGCTTTCTA AACTGGCAGA CAGAATCCAG CTGAATGGAA GGTATTATTT GAAGAACAAC
    ATGGAGACCG AAACGTTGTG TTCAGATGAA GATGCTCAGG AGCTTCTGCG TGAAAGCCTC
    ATCTCTCTCC TTCAGCTTAG CACTATCGAG GTGGCAACAC AGCTTTCTAT GCGGGACTTT
    GAACTCTTCC GCAATATTGA GCCCACAGAA TACATAGATG ACCTTTTCAA GATAGATTCC
    AAGACAAGTG CTGCTCATCT GAAACAGTTT GAGGAAGTGA TCAACCAAGA GACATTTTGG
    GTGGCCACGG AAATTCTAAG AGAACCAAAC CATTTGAAGA GAATGAAAAT TGTGAAGCAT
    TTCATAAAGA CTGCCCTCCA TTGCAGAGAA TGCAGAAATT TTAACTCCAT GTTTGCTATA
    ATAAGTGGGC TCAATCTTGC ACCTGTGGCT CGGCTTCGAG GAACTTGGGA AAAGTTGCCC
    AGCAAATACG AGAAGCTGCT TCGAGACCTG CAAGATCTCT TCGACCCCTC AAGAAATATG
    GCGAAATACC GTAATATTCT AAGCAGTCAG AGCATGCAGC CCCCCATCAT TCCTCTCTTC
    CCAGTCGTCA AGAAGGATAT AACATTTCTT CATGAAGGAA ATGATTCAAA AGTTGATGGG
    TTAGTAAATT TTGAGAAGCT GCGAATGATT GCAAAGGAGA TTCGACATGT TATTCGCATG
    ACTTCTGCTA ATATGGATCC AGCAGTTATG TTTAGGCAAA GGTCCCTAAG CCAAGGCAGC
    ACAAACTCTA ACATGCTGGA TGTTCAAGGA GGTGGTCACA AGAAACGGGC TCGGCGAAGT
    TCATTCCTTA ATGCTAAGAA GCTGTATGAA GATGCACAGA TGTCAAGGAA GGTAAAACAG
    TACATCTCCA ACCTGAGTGT GGAAATCGAT GAGGAGAAGA TCCAGCTAAT GTCACTGCAA
    TGTGAACCAG CCTACAGCAC TTTGGCAAAG AATTTAAGTG AGAGAAGATC TGCAAAATCT
    GACTTGTCTC CTGCACCTTT GAGGCCATCA GGCCAAACTA CCAAAGCTCA CATCCATCAG
    CAAAACAGAA TGAGCCAAGT ATTGCAAGTC CCTACAGTTA ACTTATATCC AATGCGAAAG
    AAAGCACCAA CCAAGGAACA TCTTCCATGC ACTTCAGGAT CTTCACAGAA AATATCTGGT
    TTGACAGATG AGGTTACAGT TAAAGCACAG TTAGAAGACT CTGTTTCTCT TGTGTCATCA
    TTGCATTCCA GCCCAGCCGC ATCCCCTCAA GGATCTCCAC ATAAAGTTGT TAACATTTCA
    AGATCAGAGA ATTCCAGTCA TATGAACCTC TCTGGATCCT CCTCATCATT AACCAGTGAT
    CCCAACCAGA AGATCTGCAG TCGGCGTGGC TATGGAATAG GCTATGCCCA TAACCCCTCG
    GCCAAATCTG ATAACTTCTC AGACTCAAGC AACAGTGAGA TCTCGTCCCG ATCAAGCCTC
    GTCAGCAACT GCTCAGTAGA CTCCATGTCG TTGACCCTGC AGGATGAAAA GTGTGTGCCT
    CATTCACATG CTGAGTCAAT GGGACTATCA GAAAGAAAAG ACGTCCATCC GTCCTCTGAG
    TATAACCAGC CCTGCAGTAG CTGGCTCCAT GCAAGGCACC CTGCAGTCCG GAATTTCATA
    GCTTGCGCAA GCAGTGAGGA CATAGCTCAT GACCACATAT CGGTAGAGCC GGTAGACAGT
    GGTCGGGGCA GTTGGACATC TTGTTCTAGC AGTTCACATG ACAATTTCCA GACTTTGCAG
    AAGGGACTGG ACCGCTTAAG CACTTACCGC CAATCACACA TTGATTCCCC AATTGCTGAG
    ACAGAGTCCG CAAACTGCAG CACAGATGAA ACATGTAGTG AAGACTGTAC CAAAGGAAAA
    AGGCACTCAA ACAACAGTTT AGAACTAAAT GAATCTCGGC AAAGTTGGGC TTCATCTAGC
    TCAATGTCAG ATACTTACGA GACGAACTAT GGCACAATCA AGCGGAGAGT ATTGGACACA
    GAGGAAAAAA CAGAGGCTTT GGGCCAACAA TTAGATGTCT TTTATAAAAC AGTTACTTCC
    ACTACTGAAA AAGGACTGAT AGTGTACTGT GTCACCTCAT CCCAGAAGGA CAGTAGGTAT
    AGGGAGCCAC CTCCCACTCC TCCAGGCTAC CAGGGGATAT CATTAGCCGA CATAAAGGAA
    GGACAACCCC ACCAAGCGCA CTTAAAACCT CCAGATTATA GTGTGGCGAT ACAGAGGTCA
    AAGATGATAC ATAAAGACCT CTCTAGGCTT TCATCAGCCT GTGTTGAACC AGTCTCATGT
    GTCTCACAGA AGATGATAAC CCATTGGCAT AACCGGCATC AGCCTTATCC TAATGTACAG
    GCTGACAGAG ATAGTAAAGA AGATGAAGAT GAACAAATGT CAGCAGTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa43156
    Clone ID Related Accession (Same CDS sequence) XM_018092133.1 , XM_018092133.2
    Accession Version XM_018092133.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4896bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 6 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTGCCA GCATTGAACC CAGGTTTAAA GAAGCCTTGA GGAAAAAACC CAAGGAAAGG 
    ACTCAGGAGG ATCTGAAAAC TGTTTATTCA TATATGCATA GAATGGATAT ACTCTCACAT
    CTCAGAGAAC ATCAATTAAG ATTGATGTCT GCAACAGCAC GATATGAAAG ATACAGTGCA
    AATCAGGTTC TATTCTGCTC AGAAAGTTTG GCTAGATGTT GGTACATTCT TCTTTCTGGT
    TCAGTTCTGG TAAAAAACTC CATGTTTTTG CCTCCATGCA GTTTTGGCAA GCAGTCAGGT
    GGCAAAAGGG GTTGTGATTG CATTGTACTG GAACCTTCTG AAATGATTGT GGTGGAAAAT
    ACAGGAGAAA ACAATGATGG CATTCTGCAT AGGGAAACTT CTATTAGACA ATCCCGGCGT
    CGGTATCGAA AAATCAACCA GCATGGAGAA CGCCAAACAA TTACAGATAA TGGGGATGCT
    GCTAGTTATG TCTTGCTCCC CACAGACCTC TCCAAGATAC ACCTCACCGA CAGTCCCCAC
    CCACATGTTA CACATGTGCC ATCCAGCCAA TCAGGATGCA GCATTGCAAG CGACTCTGGA
    AGCAGCAGCT TATCAGATAT CTACCAGGCA ACAGAAAGTG AGATGGGGGA TGTTGACTTG
    ACAGGCCTGC CAGAGGCAGC TGTTGATTCT GAAGATGAAG AGGAAGATGA AGATATTGAG
    AGAGCTTCAG ACACAATGCT GACCCGTGAC ATTGTGAGGG AATGTCTTGA GAAAGAACCC
    ACAGACAGAA CAGATGATGA CATAGAACAG CTACTGGAAT TCATGCATCA GCTTCCAGCC
    TTTGTCAATA TGACCATGTC TGTGAGACGA AAGCTTTGCT CGGTCATGAT TTTTGAAGTT
    GTGGAACAGG CTGGCGCAAT CATCATTGAA GATGGGCAAG AACTGGACTC TTGGTATGTC
    ATTCTTAATG GGATTGTAGA AATCAGCCAT TCAGATGGCA GGATGGAAAA CCTTTGCATG
    GGAAATAGCT TTGGCATAAA CCCATCTTTA GAAACACAGT ACATGGATGG AGTAGTAAAA
    ACCAAAGCTG ATGATTGCCA GTTTGTGTGC ATAGCACAAC AGGACTACTT TCGGATATTA
    AACCATGTGG AAAAAAATAC CCACAAAGTC AAGGAAAAGG GGGAGATTGT TATGGTTAAT
    GAACACCGAG AACTGGATCG AAGTGGCACT CGAAAGGGAC ATATAGTAAT TCAGGGGACT
    CCAGAAAGAC TCATCTTGCA CCTAATAGAA GAGCACTCTG TTGTTGACCC AACATACATA
    GAGGATTTTC TATTAACATA CAGGACATTC CTTTCAAGTC CATTAGAGGT TGGGGACAAG
    CTTTTGGAGT GGTTTAAAGA TGACAGTCGT CGTGACAAGG TAACACGAGT TGTCCTTCTT
    TGGGTTAACA ACCACTTTAA TGATTTTGAA GGAGACCCTG CCATGACAAG GTTTTTGGAA
    GAATTTGAAC GGAATCTTGA AAATGCGAAA ATGAAGGGTC ATCTGCGACT GCTAAACATA
    GCCTGTGCTG CAAAGGCTAA ACTGAGACAG GTTACATTGC TTAAGCCTTC CCGTGAATCG
    CCTTTTTATT TCAACGTCGT TGGGGGAAGC GACAAAGGTT TCGGCATGTT TATTGAAAGT
    GTTGAGCCTG GAAGCAAAGC AACAGAAGCT GGATTAAAAC GCGGAGATCA GATTATGGAA
    GTCAATGGCC AAAATTTTGA GACTATCGTT TACGCCAAAG CCCTGGAGAT CTTGAAAAAC
    AATACACATC TTTCCATCAC TGTCAAGACC AACATTTTTG TGTTTAAAGA GCTTCTTAAT
    CGAATCGGGC AGGAGAAAAA AAATGGAGTC CCACACATCC CCAAAATTGC AGAGAAGAAG
    AACAATAGAT ATTCCATCCC TGATCTCCCC AATGATGTAG AACAGATGTT TCCACGGGAT
    CGCGCAAGCA AGAAAATGAA AGCCAATACG GTGTCTGGAG GGCGGAATCG CATCCGGAAG
    ATTTTGGACA AAACACGTTT TAGTATATTA CCACCTAAGC CATTCAGTGA TGGCCATGTT
    ATCCAGTCCC AGGATGACAG TATTGTTGGC ACCAGGCAGT GCAGGCACAG TGTGGCTATA
    ATGCCCATTC CGGGCACGCT CTCTTCAAGC AGTCCAGACT TGCTTCAAGC TACAACAAAC
    ATATTGGATT TCTCCAATCC TACTGATATT CCAGACCAAG TTATACGAAT ATTTAAAGCA
    GATCAGCAGT GCTGCTATAT TATCATCGGC AAAGACACTA CTGCAAAGGA GGTGGTGATA
    CAAGCACTTC ATGAATTCAG CCTTACTGGG TCAACAGACA CATACTCTCT CTGTGAGGTC
    TCGGTCAGTC CAGAAGGTGT CATTAAACAA AGACGGCTTC CGGATCAGCT TTCTAAACTG
    GCAGACAGAA TCCAGCTGAA TGGAAGGTAT TATTTGAAGA ACAACATGGA GACCGAAACG
    TTGTGTTCAG ATGAAGATGC TCAGGAGCTT CTGCGTGAAA GCCTCATCTC TCTCCTTCAG
    CTTAGCACTA TCGAGGTGGC AACACAGCTT TCTATGCGGG ACTTTGAACT CTTCCGCAAT
    ATTGAGCCCA CAGAATACAT AGATGACCTT TTCAAGATAG ATTCCAAGAC AAGTGCTGCT
    CATCTGAAAC AGTTTGAGGA AGTGATCAAC CAAGAGACAT TTTGGGTGGC CACGGAAATT
    CTAAGAGAAC CAAACCATTT GAAGAGAATG AAAATTGTGA AGCATTTCAT AAAGACTGCC
    CTCCATTGCA GAGAATGCAG AAATTTTAAC TCCATGTTTG CTATAATAAG TGGGCTCAAT
    CTTGCACCTG TGGCTCGGCT TCGAGGAACT TGGGAAAAGT TGCCCAGCAA ATACGAGAAG
    CTGCTTCGAG ACCTGCAAGA TCTCTTCGAC CCCTCAAGAA ATATGGCGAA ATACCGTAAT
    ATTCTAAGCA GTCAGAGCAT GCAGCCCCCC ATCATTCCTC TCTTCCCAGT CGTCAAGAAG
    GATATAACAT TTCTTCATGA AGGAAATGAT TCAAAAGTTG ATGGGTTAGT AAATTTTGAG
    AAGCTGCGAA TGATTGCAAA GGAGATTCGA CATGTTATTC GCATGACTTC TGCTAATATG
    GATCCAGCAG TTATGTTTAG GCAAAGGTCC CTAAGCCAAG GCAGCACAAA CTCTAACATG
    CTGGATGTTC AAGGAGGTGG TCACAAGAAA CGGGCTCGGC GAAGTTCATT CCTTAATGCT
    AAGAAGCTGT ATGAAGATGC ACAGATGTCA AGGAAGGTAA AACAGTACAT CTCCAACCTG
    AGTGTGGAAA TCGATGAGGA GAAGATCCAG CTAATGTCAC TGCAATGTGA ACCAGCCTAC
    AGCACTTTGG CAAAGAATTT AAGTGAGAGA AGATCTGCAA AATCTGACTT GTCTCCTGCA
    CCTTTGAGGC CATCAGGCCA AACTACCAAA GCTCACATCC ATCAGCAAAA CAGAATGAGC
    CAAGTATTGC AAGTCCCTAC AGTTAACTTA TATCCAATGC GAAAGAAAGC ACCAACCAAG
    GAACATCTTC CATGCACTTC AGGATCTTCA CAGAAAATAT CTGGTTTGAC AGATGAGGTT
    ACAGTTAAAG CACAGTTAGA AGACTCTGTT TCTCTTGTGT CATCATTGCA TTCCAGCCCA
    GCCGCATCCC CTCAAGGATC TCCACATAAA GTTGTTAACA TTTCAAGATC AGAGAATTCC
    AGTCATATGA ACCTCTCTGG ATCCTCCTCA TCATTAACCA GTGATCCCAA CCAGAAGATC
    TGCAGTCGGC GTGGCTATGG AATAGGCTAT GCCCATAACC CCTCGGCCAA ATCTGATAAC
    TTCTCAGACT CAAGCAACAG TGAGATCTCG TCCCGATCAA GCCTCGTCAG CAACTGCTCA
    GTAGACTCCA TGTCGTTGAC CCTGCAGGAT GAAAAGTGTG TGCCTCATTC ACATGCTGAG
    TCAATGGGAC TATCAGAAAG AAAAGACGTC CATCCGTCCT CTGAGTATAA CCAGCCCTGC
    AGTAGCTGGC TCCATGCAAG GCACCCTGCA GTCCGGAATT TCATAGCTTG CGCAAGCAGT
    GAGGACATAG CTCATGACCA CATATCGGTA GAGCCGGTAG ACAGTGGTCG GGGCAGTTGG
    ACATCTTGTT CTAGCAGTTC ACATGACAAT TTCCAGACTT TGCAGAAGGG ACTGGACCGC
    TTAAGCACTT ACCGCCAATC ACACATTGAT TCCCCAATTG CTGAGACAGA GTCCGCAAAC
    TGCAGCACAG ATGAAACATG TAGTGAAGAC TGTACCAAAG GAAAAAGGCA CTCAAACAAC
    AGTTTAGAAC TAAATGAATC TCGGCAAAGT TGGGCTTCAT CTAGCTCAAT GTCAGATACT
    TACGAGACGA ACTATGGCAC AATCAAGCGG AGAGTATTGG ACACAGAGGA AAAAACAGAG
    GCTTTGGGCC AACAATTAGA TGTCTTTTAT AAAACAGTTA CTTCCACTAC TGAAAAAGGA
    CTGATAGTGT ACTGTGTCAC CTCATCCCAG AAGGACAGTA GGTATAGGGA GCCACCTCCC
    ACTCCTCCAG GCTACCAGGG GATATCATTA GCCGACATAA AGGAAGGACA ACCCCACCAA
    GCGCACTTAA AACCTCCAGA TTATAGTGTG GCGATACAGA GGTCAAAGAT GATACATAAA
    GACCTCTCTA GGCTTTCATC AGCCTGTGTT GAACCAGTCT CATGTGTCTC ACAGAAGATG
    ATAACCCATT GGCATAACCG GCATCAGCCT TATCCTAATG TACAGGCTGA CAGAGATAGT
    AAAGAAGATG AAGATGAACA AATGTCAGCA GTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947622.1
    CDS1356..6251
    Translation

    Target ORF information:

    RefSeq Version XM_018092133.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 6 (rapgef6), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092133.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTGCCA GCATTGAACC CAGGTTTAAA GAAGCCTTGA GGAAAAAACC CAAGGAAAGG 
    ACTCAGGAGG ATCTGAAAAC TGTTTATTCA TATATGCATA GAATGGATAT ACTCTCACAT
    CTCAGAGAAC ATCAATTAAG ATTGATGTCT GCAACAGCAC GATATGAAAG ATACAGTGCA
    AATCAGGTTC TATTCTGCTC AGAAAGTTTG GCTAGATGTT GGTACATTCT TCTTTCTGGT
    TCAGTTCTGG TAAAAAACTC CATGTTTTTG CCTCCATGCA GTTTTGGCAA GCAGTCAGGT
    GGCAAAAGGG GTTGTGATTG CATTGTACTG GAACCTTCTG AAATGATTGT GGTGGAAAAT
    ACAGGAGAAA ACAATGATGG CATTCTGCAT AGGGAAACTT CTATTAGACA ATCCCGGCGT
    CGGTATCGAA AAATCAACCA GCATGGAGAA CGCCAAACAA TTACAGATAA TGGGGATGCT
    GCTAGTTATG TCTTGCTCCC CACAGACCTC TCCAAGATAC ACCTCACCGA CAGTCCCCAC
    CCACATGTTA CACATGTGCC ATCCAGCCAA TCAGGATGCA GCATTGCAAG CGACTCTGGA
    AGCAGCAGCT TATCAGATAT CTACCAGGCA ACAGAAAGTG AGATGGGGGA TGTTGACTTG
    ACAGGCCTGC CAGAGGCAGC TGTTGATTCT GAAGATGAAG AGGAAGATGA AGATATTGAG
    AGAGCTTCAG ACACAATGCT GACCCGTGAC ATTGTGAGGG AATGTCTTGA GAAAGAACCC
    ACAGACAGAA CAGATGATGA CATAGAACAG CTACTGGAAT TCATGCATCA GCTTCCAGCC
    TTTGTCAATA TGACCATGTC TGTGAGACGA AAGCTTTGCT CGGTCATGAT TTTTGAAGTT
    GTGGAACAGG CTGGCGCAAT CATCATTGAA GATGGGCAAG AACTGGACTC TTGGTATGTC
    ATTCTTAATG GGATTGTAGA AATCAGCCAT TCAGATGGCA GGATGGAAAA CCTTTGCATG
    GGAAATAGCT TTGGCATAAA CCCATCTTTA GAAACACAGT ACATGGATGG AGTAGTAAAA
    ACCAAAGCTG ATGATTGCCA GTTTGTGTGC ATAGCACAAC AGGACTACTT TCGGATATTA
    AACCATGTGG AAAAAAATAC CCACAAAGTC AAGGAAAAGG GGGAGATTGT TATGGTTAAT
    GAACACCGAG AACTGGATCG AAGTGGCACT CGAAAGGGAC ATATAGTAAT TCAGGGGACT
    CCAGAAAGAC TCATCTTGCA CCTAATAGAA GAGCACTCTG TTGTTGACCC AACATACATA
    GAGGATTTTC TATTAACATA CAGGACATTC CTTTCAAGTC CATTAGAGGT TGGGGACAAG
    CTTTTGGAGT GGTTTAAAGA TGACAGTCGT CGTGACAAGG TAACACGAGT TGTCCTTCTT
    TGGGTTAACA ACCACTTTAA TGATTTTGAA GGAGACCCTG CCATGACAAG GTTTTTGGAA
    GAATTTGAAC GGAATCTTGA AAATGCGAAA ATGAAGGGTC ATCTGCGACT GCTAAACATA
    GCCTGTGCTG CAAAGGCTAA ACTGAGACAG GTTACATTGC TTAAGCCTTC CCGTGAATCG
    CCTTTTTATT TCAACGTCGT TGGGGGAAGC GACAAAGGTT TCGGCATGTT TATTGAAAGT
    GTTGAGCCTG GAAGCAAAGC AACAGAAGCT GGATTAAAAC GCGGAGATCA GATTATGGAA
    GTCAATGGCC AAAATTTTGA GACTATCGTT TACGCCAAAG CCCTGGAGAT CTTGAAAAAC
    AATACACATC TTTCCATCAC TGTCAAGACC AACATTTTTG TGTTTAAAGA GCTTCTTAAT
    CGAATCGGGC AGGAGAAAAA AAATGGAGTC CCACACATCC CCAAAATTGC AGAGAAGAAG
    AACAATAGAT ATTCCATCCC TGATCTCCCC AATGATGTAG AACAGATGTT TCCACGGGAT
    CGCGCAAGCA AGAAAATGAA AGCCAATACG GTGTCTGGAG GGCGGAATCG CATCCGGAAG
    ATTTTGGACA AAACACGTTT TAGTATATTA CCACCTAAGC CATTCAGTGA TGGCCATGTT
    ATCCAGTCCC AGGATGACAG TATTGTTGGC ACCAGGCAGT GCAGGCACAG TGTGGCTATA
    ATGCCCATTC CGGGCACGCT CTCTTCAAGC AGTCCAGACT TGCTTCAAGC TACAACAAAC
    ATATTGGATT TCTCCAATCC TACTGATATT CCAGACCAAG TTATACGAAT ATTTAAAGCA
    GATCAGCAGT GCTGCTATAT TATCATCGGC AAAGACACTA CTGCAAAGGA GGTGGTGATA
    CAAGCACTTC ATGAATTCAG CCTTACTGGG TCAACAGACA CATACTCTCT CTGTGAGGTC
    TCGGTCAGTC CAGAAGGTGT CATTAAACAA AGACGGCTTC CGGATCAGCT TTCTAAACTG
    GCAGACAGAA TCCAGCTGAA TGGAAGGTAT TATTTGAAGA ACAACATGGA GACCGAAACG
    TTGTGTTCAG ATGAAGATGC TCAGGAGCTT CTGCGTGAAA GCCTCATCTC TCTCCTTCAG
    CTTAGCACTA TCGAGGTGGC AACACAGCTT TCTATGCGGG ACTTTGAACT CTTCCGCAAT
    ATTGAGCCCA CAGAATACAT AGATGACCTT TTCAAGATAG ATTCCAAGAC AAGTGCTGCT
    CATCTGAAAC AGTTTGAGGA AGTGATCAAC CAAGAGACAT TTTGGGTGGC CACGGAAATT
    CTAAGAGAAC CAAACCATTT GAAGAGAATG AAAATTGTGA AGCATTTCAT AAAGACTGCC
    CTCCATTGCA GAGAATGCAG AAATTTTAAC TCCATGTTTG CTATAATAAG TGGGCTCAAT
    CTTGCACCTG TGGCTCGGCT TCGAGGAACT TGGGAAAAGT TGCCCAGCAA ATACGAGAAG
    CTGCTTCGAG ACCTGCAAGA TCTCTTCGAC CCCTCAAGAA ATATGGCGAA ATACCGTAAT
    ATTCTAAGCA GTCAGAGCAT GCAGCCCCCC ATCATTCCTC TCTTCCCAGT CGTCAAGAAG
    GATATAACAT TTCTTCATGA AGGAAATGAT TCAAAAGTTG ATGGGTTAGT AAATTTTGAG
    AAGCTGCGAA TGATTGCAAA GGAGATTCGA CATGTTATTC GCATGACTTC TGCTAATATG
    GATCCAGCAG TTATGTTTAG GCAAAGGTCC CTAAGCCAAG GCAGCACAAA CTCTAACATG
    CTGGATGTTC AAGGAGGTGG TCACAAGAAA CGGGCTCGGC GAAGTTCATT CCTTAATGCT
    AAGAAGCTGT ATGAAGATGC ACAGATGTCA AGGAAGGTAA AACAGTACAT CTCCAACCTG
    AGTGTGGAAA TCGATGAGGA GAAGATCCAG CTAATGTCAC TGCAATGTGA ACCAGCCTAC
    AGCACTTTGG CAAAGAATTT AAGTGAGAGA AGATCTGCAA AATCTGACTT GTCTCCTGCA
    CCTTTGAGGC CATCAGGCCA AACTACCAAA GCTCACATCC ATCAGCAAAA CAGAATGAGC
    CAAGTATTGC AAGTCCCTAC AGTTAACTTA TATCCAATGC GAAAGAAAGC ACCAACCAAG
    GAACATCTTC CATGCACTTC AGGATCTTCA CAGAAAATAT CTGGTTTGAC AGATGAGGTT
    ACAGTTAAAG CACAGTTAGA AGACTCTGTT TCTCTTGTGT CATCATTGCA TTCCAGCCCA
    GCCGCATCCC CTCAAGGATC TCCACATAAA GTTGTTAACA TTTCAAGATC AGAGAATTCC
    AGTCATATGA ACCTCTCTGG ATCCTCCTCA TCATTAACCA GTGATCCCAA CCAGAAGATC
    TGCAGTCGGC GTGGCTATGG AATAGGCTAT GCCCATAACC CCTCGGCCAA ATCTGATAAC
    TTCTCAGACT CAAGCAACAG TGAGATCTCG TCCCGATCAA GCCTCGTCAG CAACTGCTCA
    GTAGACTCCA TGTCGTTGAC CCTGCAGGAT GAAAAGTGTG TGCCTCATTC ACATGCTGAG
    TCAATGGGAC TATCAGAAAG AAAAGACGTC CATCCGTCCT CTGAGTATAA CCAGCCCTGC
    AGTAGCTGGC TCCATGCAAG GCACCCTGCA GTCCGGAATT TCATAGCTTG CGCAAGCAGT
    GAGGACATAG CTCATGACCA CATATCGGTA GAGCCGGTAG ACAGTGGTCG GGGCAGTTGG
    ACATCTTGTT CTAGCAGTTC ACATGACAAT TTCCAGACTT TGCAGAAGGG ACTGGACCGC
    TTAAGCACTT ACCGCCAATC ACACATTGAT TCCCCAATTG CTGAGACAGA GTCCGCAAAC
    TGCAGCACAG ATGAAACATG TAGTGAAGAC TGTACCAAAG GAAAAAGGCA CTCAAACAAC
    AGTTTAGAAC TAAATGAATC TCGGCAAAGT TGGGCTTCAT CTAGCTCAAT GTCAGATACT
    TACGAGACGA ACTATGGCAC AATCAAGCGG AGAGTATTGG ACACAGAGGA AAAAACAGAG
    GCTTTGGGCC AACAATTAGA TGTCTTTTAT AAAACAGTTA CTTCCACTAC TGAAAAAGGA
    CTGATAGTGT ACTGTGTCAC CTCATCCCAG AAGGACAGTA GGTATAGGGA GCCACCTCCC
    ACTCCTCCAG GCTACCAGGG GATATCATTA GCCGACATAA AGGAAGGACA ACCCCACCAA
    GCGCACTTAA AACCTCCAGA TTATAGTGTG GCGATACAGA GGTCAAAGAT GATACATAAA
    GACCTCTCTA GGCTTTCATC AGCCTGTGTT GAACCAGTCT CATGTGTCTC ACAGAAGATG
    ATAACCCATT GGCATAACCG GCATCAGCCT TATCCTAATG TACAGGCTGA CAGAGATAGT
    AAAGAAGATG AAGATGAACA AATGTCAGCA GTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa43156
    Clone ID Related Accession (Same CDS sequence) XM_018092133.1 , XM_018092133.2
    Accession Version XM_018092133.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4896bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 6 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018092133.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTGCCA GCATTGAACC CAGGTTTAAA GAAGCCTTGA GGAAAAAACC CAAGGAAAGG 
    ACTCAGGAGG ATCTGAAAAC TGTTTATTCA TATATGCATA GAATGGATAT ACTCTCACAT
    CTCAGAGAAC ATCAATTAAG ATTGATGTCT GCAACAGCAC GATATGAAAG ATACAGTGCA
    AATCAGGTTC TATTCTGCTC AGAAAGTTTG GCTAGATGTT GGTACATTCT TCTTTCTGGT
    TCAGTTCTGG TAAAAAACTC CATGTTTTTG CCTCCATGCA GTTTTGGCAA GCAGTCAGGT
    GGCAAAAGGG GTTGTGATTG CATTGTACTG GAACCTTCTG AAATGATTGT GGTGGAAAAT
    ACAGGAGAAA ACAATGATGG CATTCTGCAT AGGGAAACTT CTATTAGACA ATCCCGGCGT
    CGGTATCGAA AAATCAACCA GCATGGAGAA CGCCAAACAA TTACAGATAA TGGGGATGCT
    GCTAGTTATG TCTTGCTCCC CACAGACCTC TCCAAGATAC ACCTCACCGA CAGTCCCCAC
    CCACATGTTA CACATGTGCC ATCCAGCCAA TCAGGATGCA GCATTGCAAG CGACTCTGGA
    AGCAGCAGCT TATCAGATAT CTACCAGGCA ACAGAAAGTG AGATGGGGGA TGTTGACTTG
    ACAGGCCTGC CAGAGGCAGC TGTTGATTCT GAAGATGAAG AGGAAGATGA AGATATTGAG
    AGAGCTTCAG ACACAATGCT GACCCGTGAC ATTGTGAGGG AATGTCTTGA GAAAGAACCC
    ACAGACAGAA CAGATGATGA CATAGAACAG CTACTGGAAT TCATGCATCA GCTTCCAGCC
    TTTGTCAATA TGACCATGTC TGTGAGACGA AAGCTTTGCT CGGTCATGAT TTTTGAAGTT
    GTGGAACAGG CTGGCGCAAT CATCATTGAA GATGGGCAAG AACTGGACTC TTGGTATGTC
    ATTCTTAATG GGATTGTAGA AATCAGCCAT TCAGATGGCA GGATGGAAAA CCTTTGCATG
    GGAAATAGCT TTGGCATAAA CCCATCTTTA GAAACACAGT ACATGGATGG AGTAGTAAAA
    ACCAAAGCTG ATGATTGCCA GTTTGTGTGC ATAGCACAAC AGGACTACTT TCGGATATTA
    AACCATGTGG AAAAAAATAC CCACAAAGTC AAGGAAAAGG GGGAGATTGT TATGGTTAAT
    GAACACCGAG AACTGGATCG AAGTGGCACT CGAAAGGGAC ATATAGTAAT TCAGGGGACT
    CCAGAAAGAC TCATCTTGCA CCTAATAGAA GAGCACTCTG TTGTTGACCC AACATACATA
    GAGGATTTTC TATTAACATA CAGGACATTC CTTTCAAGTC CATTAGAGGT TGGGGACAAG
    CTTTTGGAGT GGTTTAAAGA TGACAGTCGT CGTGACAAGG TAACACGAGT TGTCCTTCTT
    TGGGTTAACA ACCACTTTAA TGATTTTGAA GGAGACCCTG CCATGACAAG GTTTTTGGAA
    GAATTTGAAC GGAATCTTGA AAATGCGAAA ATGAAGGGTC ATCTGCGACT GCTAAACATA
    GCCTGTGCTG CAAAGGCTAA ACTGAGACAG GTTACATTGC TTAAGCCTTC CCGTGAATCG
    CCTTTTTATT TCAACGTCGT TGGGGGAAGC GACAAAGGTT TCGGCATGTT TATTGAAAGT
    GTTGAGCCTG GAAGCAAAGC AACAGAAGCT GGATTAAAAC GCGGAGATCA GATTATGGAA
    GTCAATGGCC AAAATTTTGA GACTATCGTT TACGCCAAAG CCCTGGAGAT CTTGAAAAAC
    AATACACATC TTTCCATCAC TGTCAAGACC AACATTTTTG TGTTTAAAGA GCTTCTTAAT
    CGAATCGGGC AGGAGAAAAA AAATGGAGTC CCACACATCC CCAAAATTGC AGAGAAGAAG
    AACAATAGAT ATTCCATCCC TGATCTCCCC AATGATGTAG AACAGATGTT TCCACGGGAT
    CGCGCAAGCA AGAAAATGAA AGCCAATACG GTGTCTGGAG GGCGGAATCG CATCCGGAAG
    ATTTTGGACA AAACACGTTT TAGTATATTA CCACCTAAGC CATTCAGTGA TGGCCATGTT
    ATCCAGTCCC AGGATGACAG TATTGTTGGC ACCAGGCAGT GCAGGCACAG TGTGGCTATA
    ATGCCCATTC CGGGCACGCT CTCTTCAAGC AGTCCAGACT TGCTTCAAGC TACAACAAAC
    ATATTGGATT TCTCCAATCC TACTGATATT CCAGACCAAG TTATACGAAT ATTTAAAGCA
    GATCAGCAGT GCTGCTATAT TATCATCGGC AAAGACACTA CTGCAAAGGA GGTGGTGATA
    CAAGCACTTC ATGAATTCAG CCTTACTGGG TCAACAGACA CATACTCTCT CTGTGAGGTC
    TCGGTCAGTC CAGAAGGTGT CATTAAACAA AGACGGCTTC CGGATCAGCT TTCTAAACTG
    GCAGACAGAA TCCAGCTGAA TGGAAGGTAT TATTTGAAGA ACAACATGGA GACCGAAACG
    TTGTGTTCAG ATGAAGATGC TCAGGAGCTT CTGCGTGAAA GCCTCATCTC TCTCCTTCAG
    CTTAGCACTA TCGAGGTGGC AACACAGCTT TCTATGCGGG ACTTTGAACT CTTCCGCAAT
    ATTGAGCCCA CAGAATACAT AGATGACCTT TTCAAGATAG ATTCCAAGAC AAGTGCTGCT
    CATCTGAAAC AGTTTGAGGA AGTGATCAAC CAAGAGACAT TTTGGGTGGC CACGGAAATT
    CTAAGAGAAC CAAACCATTT GAAGAGAATG AAAATTGTGA AGCATTTCAT AAAGACTGCC
    CTCCATTGCA GAGAATGCAG AAATTTTAAC TCCATGTTTG CTATAATAAG TGGGCTCAAT
    CTTGCACCTG TGGCTCGGCT TCGAGGAACT TGGGAAAAGT TGCCCAGCAA ATACGAGAAG
    CTGCTTCGAG ACCTGCAAGA TCTCTTCGAC CCCTCAAGAA ATATGGCGAA ATACCGTAAT
    ATTCTAAGCA GTCAGAGCAT GCAGCCCCCC ATCATTCCTC TCTTCCCAGT CGTCAAGAAG
    GATATAACAT TTCTTCATGA AGGAAATGAT TCAAAAGTTG ATGGGTTAGT AAATTTTGAG
    AAGCTGCGAA TGATTGCAAA GGAGATTCGA CATGTTATTC GCATGACTTC TGCTAATATG
    GATCCAGCAG TTATGTTTAG GCAAAGGTCC CTAAGCCAAG GCAGCACAAA CTCTAACATG
    CTGGATGTTC AAGGAGGTGG TCACAAGAAA CGGGCTCGGC GAAGTTCATT CCTTAATGCT
    AAGAAGCTGT ATGAAGATGC ACAGATGTCA AGGAAGGTAA AACAGTACAT CTCCAACCTG
    AGTGTGGAAA TCGATGAGGA GAAGATCCAG CTAATGTCAC TGCAATGTGA ACCAGCCTAC
    AGCACTTTGG CAAAGAATTT AAGTGAGAGA AGATCTGCAA AATCTGACTT GTCTCCTGCA
    CCTTTGAGGC CATCAGGCCA AACTACCAAA GCTCACATCC ATCAGCAAAA CAGAATGAGC
    CAAGTATTGC AAGTCCCTAC AGTTAACTTA TATCCAATGC GAAAGAAAGC ACCAACCAAG
    GAACATCTTC CATGCACTTC AGGATCTTCA CAGAAAATAT CTGGTTTGAC AGATGAGGTT
    ACAGTTAAAG CACAGTTAGA AGACTCTGTT TCTCTTGTGT CATCATTGCA TTCCAGCCCA
    GCCGCATCCC CTCAAGGATC TCCACATAAA GTTGTTAACA TTTCAAGATC AGAGAATTCC
    AGTCATATGA ACCTCTCTGG ATCCTCCTCA TCATTAACCA GTGATCCCAA CCAGAAGATC
    TGCAGTCGGC GTGGCTATGG AATAGGCTAT GCCCATAACC CCTCGGCCAA ATCTGATAAC
    TTCTCAGACT CAAGCAACAG TGAGATCTCG TCCCGATCAA GCCTCGTCAG CAACTGCTCA
    GTAGACTCCA TGTCGTTGAC CCTGCAGGAT GAAAAGTGTG TGCCTCATTC ACATGCTGAG
    TCAATGGGAC TATCAGAAAG AAAAGACGTC CATCCGTCCT CTGAGTATAA CCAGCCCTGC
    AGTAGCTGGC TCCATGCAAG GCACCCTGCA GTCCGGAATT TCATAGCTTG CGCAAGCAGT
    GAGGACATAG CTCATGACCA CATATCGGTA GAGCCGGTAG ACAGTGGTCG GGGCAGTTGG
    ACATCTTGTT CTAGCAGTTC ACATGACAAT TTCCAGACTT TGCAGAAGGG ACTGGACCGC
    TTAAGCACTT ACCGCCAATC ACACATTGAT TCCCCAATTG CTGAGACAGA GTCCGCAAAC
    TGCAGCACAG ATGAAACATG TAGTGAAGAC TGTACCAAAG GAAAAAGGCA CTCAAACAAC
    AGTTTAGAAC TAAATGAATC TCGGCAAAGT TGGGCTTCAT CTAGCTCAAT GTCAGATACT
    TACGAGACGA ACTATGGCAC AATCAAGCGG AGAGTATTGG ACACAGAGGA AAAAACAGAG
    GCTTTGGGCC AACAATTAGA TGTCTTTTAT AAAACAGTTA CTTCCACTAC TGAAAAAGGA
    CTGATAGTGT ACTGTGTCAC CTCATCCCAG AAGGACAGTA GGTATAGGGA GCCACCTCCC
    ACTCCTCCAG GCTACCAGGG GATATCATTA GCCGACATAA AGGAAGGACA ACCCCACCAA
    GCGCACTTAA AACCTCCAGA TTATAGTGTG GCGATACAGA GGTCAAAGAT GATACATAAA
    GACCTCTCTA GGCTTTCATC AGCCTGTGTT GAACCAGTCT CATGTGTCTC ACAGAAGATG
    ATAACCCATT GGCATAACCG GCATCAGCCT TATCCTAATG TACAGGCTGA CAGAGATAGT
    AAAGAAGATG AAGATGAACA AATGTCAGCA GTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947622.1
    CDS484..5379
    Translation

    Target ORF information:

    RefSeq Version XM_018092133.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 6 (rapgef6), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092133.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTGCCA GCATTGAACC CAGGTTTAAA GAAGCCTTGA GGAAAAAACC CAAGGAAAGG 
    ACTCAGGAGG ATCTGAAAAC TGTTTATTCA TATATGCATA GAATGGATAT ACTCTCACAT
    CTCAGAGAAC ATCAATTAAG ATTGATGTCT GCAACAGCAC GATATGAAAG ATACAGTGCA
    AATCAGGTTC TATTCTGCTC AGAAAGTTTG GCTAGATGTT GGTACATTCT TCTTTCTGGT
    TCAGTTCTGG TAAAAAACTC CATGTTTTTG CCTCCATGCA GTTTTGGCAA GCAGTCAGGT
    GGCAAAAGGG GTTGTGATTG CATTGTACTG GAACCTTCTG AAATGATTGT GGTGGAAAAT
    ACAGGAGAAA ACAATGATGG CATTCTGCAT AGGGAAACTT CTATTAGACA ATCCCGGCGT
    CGGTATCGAA AAATCAACCA GCATGGAGAA CGCCAAACAA TTACAGATAA TGGGGATGCT
    GCTAGTTATG TCTTGCTCCC CACAGACCTC TCCAAGATAC ACCTCACCGA CAGTCCCCAC
    CCACATGTTA CACATGTGCC ATCCAGCCAA TCAGGATGCA GCATTGCAAG CGACTCTGGA
    AGCAGCAGCT TATCAGATAT CTACCAGGCA ACAGAAAGTG AGATGGGGGA TGTTGACTTG
    ACAGGCCTGC CAGAGGCAGC TGTTGATTCT GAAGATGAAG AGGAAGATGA AGATATTGAG
    AGAGCTTCAG ACACAATGCT GACCCGTGAC ATTGTGAGGG AATGTCTTGA GAAAGAACCC
    ACAGACAGAA CAGATGATGA CATAGAACAG CTACTGGAAT TCATGCATCA GCTTCCAGCC
    TTTGTCAATA TGACCATGTC TGTGAGACGA AAGCTTTGCT CGGTCATGAT TTTTGAAGTT
    GTGGAACAGG CTGGCGCAAT CATCATTGAA GATGGGCAAG AACTGGACTC TTGGTATGTC
    ATTCTTAATG GGATTGTAGA AATCAGCCAT TCAGATGGCA GGATGGAAAA CCTTTGCATG
    GGAAATAGCT TTGGCATAAA CCCATCTTTA GAAACACAGT ACATGGATGG AGTAGTAAAA
    ACCAAAGCTG ATGATTGCCA GTTTGTGTGC ATAGCACAAC AGGACTACTT TCGGATATTA
    AACCATGTGG AAAAAAATAC CCACAAAGTC AAGGAAAAGG GGGAGATTGT TATGGTTAAT
    GAACACCGAG AACTGGATCG AAGTGGCACT CGAAAGGGAC ATATAGTAAT TCAGGGGACT
    CCAGAAAGAC TCATCTTGCA CCTAATAGAA GAGCACTCTG TTGTTGACCC AACATACATA
    GAGGATTTTC TATTAACATA CAGGACATTC CTTTCAAGTC CATTAGAGGT TGGGGACAAG
    CTTTTGGAGT GGTTTAAAGA TGACAGTCGT CGTGACAAGG TAACACGAGT TGTCCTTCTT
    TGGGTTAACA ACCACTTTAA TGATTTTGAA GGAGACCCTG CCATGACAAG GTTTTTGGAA
    GAATTTGAAC GGAATCTTGA AAATGCGAAA ATGAAGGGTC ATCTGCGACT GCTAAACATA
    GCCTGTGCTG CAAAGGCTAA ACTGAGACAG GTTACATTGC TTAAGCCTTC CCGTGAATCG
    CCTTTTTATT TCAACGTCGT TGGGGGAAGC GACAAAGGTT TCGGCATGTT TATTGAAAGT
    GTTGAGCCTG GAAGCAAAGC AACAGAAGCT GGATTAAAAC GCGGAGATCA GATTATGGAA
    GTCAATGGCC AAAATTTTGA GACTATCGTT TACGCCAAAG CCCTGGAGAT CTTGAAAAAC
    AATACACATC TTTCCATCAC TGTCAAGACC AACATTTTTG TGTTTAAAGA GCTTCTTAAT
    CGAATCGGGC AGGAGAAAAA AAATGGAGTC CCACACATCC CCAAAATTGC AGAGAAGAAG
    AACAATAGAT ATTCCATCCC TGATCTCCCC AATGATGTAG AACAGATGTT TCCACGGGAT
    CGCGCAAGCA AGAAAATGAA AGCCAATACG GTGTCTGGAG GGCGGAATCG CATCCGGAAG
    ATTTTGGACA AAACACGTTT TAGTATATTA CCACCTAAGC CATTCAGTGA TGGCCATGTT
    ATCCAGTCCC AGGATGACAG TATTGTTGGC ACCAGGCAGT GCAGGCACAG TGTGGCTATA
    ATGCCCATTC CGGGCACGCT CTCTTCAAGC AGTCCAGACT TGCTTCAAGC TACAACAAAC
    ATATTGGATT TCTCCAATCC TACTGATATT CCAGACCAAG TTATACGAAT ATTTAAAGCA
    GATCAGCAGT GCTGCTATAT TATCATCGGC AAAGACACTA CTGCAAAGGA GGTGGTGATA
    CAAGCACTTC ATGAATTCAG CCTTACTGGG TCAACAGACA CATACTCTCT CTGTGAGGTC
    TCGGTCAGTC CAGAAGGTGT CATTAAACAA AGACGGCTTC CGGATCAGCT TTCTAAACTG
    GCAGACAGAA TCCAGCTGAA TGGAAGGTAT TATTTGAAGA ACAACATGGA GACCGAAACG
    TTGTGTTCAG ATGAAGATGC TCAGGAGCTT CTGCGTGAAA GCCTCATCTC TCTCCTTCAG
    CTTAGCACTA TCGAGGTGGC AACACAGCTT TCTATGCGGG ACTTTGAACT CTTCCGCAAT
    ATTGAGCCCA CAGAATACAT AGATGACCTT TTCAAGATAG ATTCCAAGAC AAGTGCTGCT
    CATCTGAAAC AGTTTGAGGA AGTGATCAAC CAAGAGACAT TTTGGGTGGC CACGGAAATT
    CTAAGAGAAC CAAACCATTT GAAGAGAATG AAAATTGTGA AGCATTTCAT AAAGACTGCC
    CTCCATTGCA GAGAATGCAG AAATTTTAAC TCCATGTTTG CTATAATAAG TGGGCTCAAT
    CTTGCACCTG TGGCTCGGCT TCGAGGAACT TGGGAAAAGT TGCCCAGCAA ATACGAGAAG
    CTGCTTCGAG ACCTGCAAGA TCTCTTCGAC CCCTCAAGAA ATATGGCGAA ATACCGTAAT
    ATTCTAAGCA GTCAGAGCAT GCAGCCCCCC ATCATTCCTC TCTTCCCAGT CGTCAAGAAG
    GATATAACAT TTCTTCATGA AGGAAATGAT TCAAAAGTTG ATGGGTTAGT AAATTTTGAG
    AAGCTGCGAA TGATTGCAAA GGAGATTCGA CATGTTATTC GCATGACTTC TGCTAATATG
    GATCCAGCAG TTATGTTTAG GCAAAGGTCC CTAAGCCAAG GCAGCACAAA CTCTAACATG
    CTGGATGTTC AAGGAGGTGG TCACAAGAAA CGGGCTCGGC GAAGTTCATT CCTTAATGCT
    AAGAAGCTGT ATGAAGATGC ACAGATGTCA AGGAAGGTAA AACAGTACAT CTCCAACCTG
    AGTGTGGAAA TCGATGAGGA GAAGATCCAG CTAATGTCAC TGCAATGTGA ACCAGCCTAC
    AGCACTTTGG CAAAGAATTT AAGTGAGAGA AGATCTGCAA AATCTGACTT GTCTCCTGCA
    CCTTTGAGGC CATCAGGCCA AACTACCAAA GCTCACATCC ATCAGCAAAA CAGAATGAGC
    CAAGTATTGC AAGTCCCTAC AGTTAACTTA TATCCAATGC GAAAGAAAGC ACCAACCAAG
    GAACATCTTC CATGCACTTC AGGATCTTCA CAGAAAATAT CTGGTTTGAC AGATGAGGTT
    ACAGTTAAAG CACAGTTAGA AGACTCTGTT TCTCTTGTGT CATCATTGCA TTCCAGCCCA
    GCCGCATCCC CTCAAGGATC TCCACATAAA GTTGTTAACA TTTCAAGATC AGAGAATTCC
    AGTCATATGA ACCTCTCTGG ATCCTCCTCA TCATTAACCA GTGATCCCAA CCAGAAGATC
    TGCAGTCGGC GTGGCTATGG AATAGGCTAT GCCCATAACC CCTCGGCCAA ATCTGATAAC
    TTCTCAGACT CAAGCAACAG TGAGATCTCG TCCCGATCAA GCCTCGTCAG CAACTGCTCA
    GTAGACTCCA TGTCGTTGAC CCTGCAGGAT GAAAAGTGTG TGCCTCATTC ACATGCTGAG
    TCAATGGGAC TATCAGAAAG AAAAGACGTC CATCCGTCCT CTGAGTATAA CCAGCCCTGC
    AGTAGCTGGC TCCATGCAAG GCACCCTGCA GTCCGGAATT TCATAGCTTG CGCAAGCAGT
    GAGGACATAG CTCATGACCA CATATCGGTA GAGCCGGTAG ACAGTGGTCG GGGCAGTTGG
    ACATCTTGTT CTAGCAGTTC ACATGACAAT TTCCAGACTT TGCAGAAGGG ACTGGACCGC
    TTAAGCACTT ACCGCCAATC ACACATTGAT TCCCCAATTG CTGAGACAGA GTCCGCAAAC
    TGCAGCACAG ATGAAACATG TAGTGAAGAC TGTACCAAAG GAAAAAGGCA CTCAAACAAC
    AGTTTAGAAC TAAATGAATC TCGGCAAAGT TGGGCTTCAT CTAGCTCAAT GTCAGATACT
    TACGAGACGA ACTATGGCAC AATCAAGCGG AGAGTATTGG ACACAGAGGA AAAAACAGAG
    GCTTTGGGCC AACAATTAGA TGTCTTTTAT AAAACAGTTA CTTCCACTAC TGAAAAAGGA
    CTGATAGTGT ACTGTGTCAC CTCATCCCAG AAGGACAGTA GGTATAGGGA GCCACCTCCC
    ACTCCTCCAG GCTACCAGGG GATATCATTA GCCGACATAA AGGAAGGACA ACCCCACCAA
    GCGCACTTAA AACCTCCAGA TTATAGTGTG GCGATACAGA GGTCAAAGAT GATACATAAA
    GACCTCTCTA GGCTTTCATC AGCCTGTGTT GAACCAGTCT CATGTGTCTC ACAGAAGATG
    ATAACCCATT GGCATAACCG GCATCAGCCT TATCCTAATG TACAGGCTGA CAGAGATAGT
    AAAGAAGATG AAGATGAACA AATGTCAGCA GTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa43157
    Clone ID Related Accession (Same CDS sequence) XM_018092134.1 , XM_018092134.2
    Accession Version XM_018092134.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4632bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 6 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTCTGCCA GCATTGAACC CAGGTTTAAA GAAGCCTTGA GGAAAAAACC CAAGGAAAGG 
    ACTCAGGAGG ATCTGAAAAC TGTTTATTCA TATATGCATA GAATGGATAT ACTCTCACAT
    CTCAGAGAAC ATCAATTAAG ATTGATGTCT GCAACAGCAC GATATGAAAG ATACAGTGCA
    AATCAGGTTC TATTCTGCTC AGAAAGTTTG GCTAGATGTT GGTACATTCT TCTTTCTGGT
    TCAGTTCTGG TAAAAAACTC CATGTTTTTG CCTCCATGCA GTTTTGGCAA GCAGTCAGGT
    GGCAAAAGGG GTTGTGATTG CATTGTACTG GAACCTTCTG AAATGATTGT GGTGGAAAAT
    ACAGGAGAAA ACAATGATGG CATTCTGCAT AGGGAAACTT CTATTAGACA ATCCCGGCGT
    CGGTATCGAA AAATCAACCA GCATGGAGAA CGCCAAACAA TTACAGATAA TGGGGATGCT
    GCTAGTTATG TCTTGCTCCC CACAGACCTC TCCAAGATAC ACCTCACCGA CAGTCCCCAC
    CCACATGTTA CACATGTGCC ATCCAGCCAA TCAGGATGCA GCATTGCAAG CGACTCTGGA
    AGCAGCAGCT TATCAGATAT CTACCAGGCA ACAGAAAGTG AGATGGGGGA TGTTGACTTG
    ACAGGCCTGC CAGAGGCAGC TGTTGATTCT GAAGATGAAG AGGAAGATGA AGATATTGAG
    AGAGCTTCAG ACACAATGCT GACCCGTGAC ATTGTGAGGG AATGTCTTGA GAAAGAACCC
    ACAGACAGAA CAGATGATGA CATAGAACAG CTACTGGAAT TCATGCATCA GCTTCCAGCC
    TTTGTCAATA TGACCATGTC TGTGAGACGA AAGCTTTGCT CGGTCATGAT TTTTGAAGTT
    GTGGAACAGG CTGGCGCAAT CATCATTGAA GATGGGCAAG AACTGGACTC TTGGTATGTC
    ATTCTTAATG GGATTGTAGA AATCAGCCAT TCAGATGGCA GGATGGAAAA CCTTTGCATG
    GGAAATAGCT TTGGCATAAA CCCATCTTTA GAAACACAGT ACATGGATGG AGTAGTAAAA
    ACCAAAGCTG ATGATTGCCA GTTTGTGTGC ATAGCACAAC AGGACTACTT TCGGATATTA
    AACCATGTGG AAAAAAATAC CCACAAAGTC AAGGAAAAGG GGGAGATTGT TATGGTTAAT
    GAACACCGAG AACTGGATCG AAGTGGCACT CGAAAGGGAC ATATAGTAAT TCAGGGGACT
    CCAGAAAGAC TCATCTTGCA CCTAATAGAA GAGCACTCTG TTGTTGACCC AACATACATA
    GAGGATTTTC TATTAACATA CAGGACATTC CTTTCAAGTC CATTAGAGGT TGGGGACAAG
    CTTTTGGAGT GGTTTAAAGA TGACAGTCGT CGTGACAAGG TAACACGAGT TGTCCTTCTT
    TGGGTTAACA ACCACTTTAA TGATTTTGAA GGAGACCCTG CCATGACAAG GTTTTTGGAA
    GAATTTGAAC GGAATCTTGA AAATGCGAAA ATGAAGGGTC ATCTGCGACT GCTAAACATA
    GCCTGTGCTG CAAAGGCTAA ACTGAGACAG GTTACATTGC TTAAGCCTTC CCGTGAATCG
    CCTTTTTATT TCAACGTCGT TGGGGGAAGC GACAAAGGTT TCGGCATGTT TATTGAAAGT
    GTTGAGCCTG GAAGCAAAGC AACAGAAGCT GGATTAAAAC GCGGAGATCA GATTATGGAA
    GTCAATGGCC AAAATTTTGA GACTATCGTT TACGCCAAAG CCCTGGAGAT CTTGAAAAAC
    AATACACATC TTTCCATCAC TGTCAAGACC AACATTTTTG TGTTTAAAGA GCTTCTTAAT
    CGAATCGGGC AGGAGAAAAA AAATGGAGTC CCACACATCC CCAAAATTGC AGAGAAGAAG
    AACAATAGAT ATTCCATCCC TGATCTCCCC AATGATGTAG AACAGATGTT TCCACGGGAT
    CGCGCAAGCA AGAAAATGAA AGCCAATACG GTGTCTGGAG GGCGGAATCG CATCCGGAAG
    ATTTTGGACA AAACACGTTT TAGTATATTA CCACCTAAGC CATTCAGTGA TGGCCATGTT
    ATCCAGTCCC AGGATGACAG TATTGTTGGC ACCAGGCAGT GCAGGCACAG TGTGGCTATA
    ATGCCCATTC CGGGCACGCT CTCTTCAAGC AGTCCAGACT TGCTTCAAGC TACAACAAAC
    ATATTGGATT TCTCCAATCC TACTGCTTTT GGATTTTACT ATATTCCAGA CCAAGTTATA
    CGAATATTTA AAGCAGATCA GCAGTGCTGC TATATTATCA TCGGCAAAGA CACTACTGCA
    AAGGAGGTGG TGATACAAGC ACTTCATGAA TTCAGCCTTA CTGGGTCAAC AGACACATAC
    TCTCTCTGTG AGGTCTCGGT CAGTCCAGAA GGTGTCATTA AACAAAGACG GCTTCCGGAT
    CAGCTTTCTA AACTGGCAGA CAGAATCCAG CTGAATGGAA GGTATTATTT GAAGAACAAC
    ATGGAGACCG AAACGTTGTG TTCAGATGAA GATGCTCAGG AGCTTCTGCG TGAAAGCCTC
    ATCTCTCTCC TTCAGCTTAG CACTATCGAG GTGGCAACAC AGCTTTCTAT GCGGGACTTT
    GAACTCTTCC GCAATATTGA GCCCACAGAA TACATAGATG ACCTTTTCAA GATAGATTCC
    AAGACAAGTG CTGCTCATCT GAAACAGTTT GAGGAAGTGA TCAACCAAGA GACATTTTGG
    GTGGCCACGG AAATTCTAAG AGAACCAAAC CATTTGAAGA GAATGAAAAT TGTGAAGCAT
    TTCATAAAGA CTGCCCTCCA TTGCAGAGAA TGCAGAAATT TTAACTCCAT GTTTGCTATA
    ATAAGTGGGC TCAATCTTGC ACCTGTGGCT CGGCTTCGAG GAACTTGGGA AAAGTTGCCC
    AGCAAATACG AGAAGCTGCT TCGAGACCTG CAAGATCTCT TCGACCCCTC AAGAAATATG
    GCGAAATACC GTAATATTCT AAGCAGTCAG AGCATGCAGC CCCCCATCAT TCCTCTCTTC
    CCAGTCGTCA AGAAGGATAT AACATTTCTT CATGAAGGAA ATGATTCAAA AGTTGATGGG
    TTAGTAAATT TTGAGAAGCT GCGAATGATT GCAAAGGAGA TTCGACATGT TATTCGCATG
    ACTTCTGCTA ATATGGATCC AGCAGTTATG TTTAGGCAAA GGAAGAAGAG ATGGAGAAGT
    TTAGGGTCCC TAAGCCAAGG CAGCACAAAC TCTAACATGC TGGATGTTCA AGGAGGTGGT
    CACAAGAAAC GGGCTCGGCG AAGTTCATTC CTTAATGCTA AGAAGCTGTA TGAAGATGCA
    CAGATGTCAA GGAAGGTAAA ACAGTACATC TCCAACCTGA GTGTGGAAAT CGATGAGGAG
    AAGATCCAGC TAATGTCACT GCAATGTGAA CCAGCCTACA GCACTTTGGC AAAGAATTTA
    AGTGAGAGAA GATCTGCAAA ATCTGACTTG TCTCCTGCAC CTTTGAGGCC ATCAGGCCAA
    ACTACCAAAG CTCACATCCA TCAGCAAAAC AGAATGAGCC AAGTATTGCA AGTCCCTACA
    GTTAACTTAT ATCCAATGCG AAAGAAAGCA CCAACCAAGG AACATCTTCC ATGCACTTCA
    GGATCTTCAC AGAAAATATC TGGTTTGACA GATGAGGTTA CAGTTAAAGC ACAGTTAGAA
    GACTCTGTTT CTCTTGTGTC ATCATTGCAT TCCAGCCCAG CCGCATCCCC TCAAGGATCT
    CCACATAAAG TTGTTAACAT TTCAAGATCA GAGAATTCCA GTCATATGAA CCTCTCTGGA
    TCCTCCTCAT CATTAACCAG TGATCCCAAC CAGAAGATCT GCAGTCGGCG TGGCTATGGA
    ATAGGCTATG CCCATAACCC CTCGGCCAAA TCTGATAACT TCTCAGACTC AAGCAACAGT
    GAGATCTCGT CCCGATCAAG CCTCGTCAGC AACTGCTCAG TAGACTCCAT GTCGTTGACC
    CTGCAGGATG AAAAGTGTGT GCCTCATTCA CATGCTGAGT CAATGGGACT ATCAGAAAGA
    AAAGACGTCC ATCCGTCCTC TGAGTATAAC CAGCCCTGCA GTAGCTGGCT CCATGCAAGG
    CACCCTGCAG TCCGGAATTT CATAGCTTGC GCAAGCAGTG AGGACATAGC TCATGACCAC
    ATATCGGTAG AGCCGGTAGA CAGTGGTCGG GGCAGTTGGA CATCTTGTTC TAGCAGTTCA
    CATGACAATT TCCAGACTTT GCAGAAGGGA CTGGACCGCT TAAGCACTTA CCGCCAATCA
    CACATTGATT CCCCAATTGC TGAGACAGAG TCCGCAAACT GCAGCACAGA TGAAACATGT
    AGTGAAGACT GTACCAAAGG AAAAAGGCAC TCAAACAACA GTTTAGAACT AAATGAATCT
    CGGCAAAGTT GGGCTTCATC TAGCTCAATG TCAGATACTT ACGAGACGAA CTATGGCACA
    ATCAAGCGGA GAGTATTGGA CACAGAGGAA AAAACAGAGG CTTTGGGCCA ACAATTAGAT
    GTCTTTTATA AAACAGTTAC TTCCACTACT GAAAAAGGAC TGATAGAAGA TGAACAAATG
    TCAGCAGTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947623.1
    CDS1359..5990
    Translation

    Target ORF information:

    RefSeq Version XM_018092134.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 6 (rapgef6), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092134.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTCTGCCA GCATTGAACC CAGGTTTAAA GAAGCCTTGA GGAAAAAACC CAAGGAAAGG 
    ACTCAGGAGG ATCTGAAAAC TGTTTATTCA TATATGCATA GAATGGATAT ACTCTCACAT
    CTCAGAGAAC ATCAATTAAG ATTGATGTCT GCAACAGCAC GATATGAAAG ATACAGTGCA
    AATCAGGTTC TATTCTGCTC AGAAAGTTTG GCTAGATGTT GGTACATTCT TCTTTCTGGT
    TCAGTTCTGG TAAAAAACTC CATGTTTTTG CCTCCATGCA GTTTTGGCAA GCAGTCAGGT
    GGCAAAAGGG GTTGTGATTG CATTGTACTG GAACCTTCTG AAATGATTGT GGTGGAAAAT
    ACAGGAGAAA ACAATGATGG CATTCTGCAT AGGGAAACTT CTATTAGACA ATCCCGGCGT
    CGGTATCGAA AAATCAACCA GCATGGAGAA CGCCAAACAA TTACAGATAA TGGGGATGCT
    GCTAGTTATG TCTTGCTCCC CACAGACCTC TCCAAGATAC ACCTCACCGA CAGTCCCCAC
    CCACATGTTA CACATGTGCC ATCCAGCCAA TCAGGATGCA GCATTGCAAG CGACTCTGGA
    AGCAGCAGCT TATCAGATAT CTACCAGGCA ACAGAAAGTG AGATGGGGGA TGTTGACTTG
    ACAGGCCTGC CAGAGGCAGC TGTTGATTCT GAAGATGAAG AGGAAGATGA AGATATTGAG
    AGAGCTTCAG ACACAATGCT GACCCGTGAC ATTGTGAGGG AATGTCTTGA GAAAGAACCC
    ACAGACAGAA CAGATGATGA CATAGAACAG CTACTGGAAT TCATGCATCA GCTTCCAGCC
    TTTGTCAATA TGACCATGTC TGTGAGACGA AAGCTTTGCT CGGTCATGAT TTTTGAAGTT
    GTGGAACAGG CTGGCGCAAT CATCATTGAA GATGGGCAAG AACTGGACTC TTGGTATGTC
    ATTCTTAATG GGATTGTAGA AATCAGCCAT TCAGATGGCA GGATGGAAAA CCTTTGCATG
    GGAAATAGCT TTGGCATAAA CCCATCTTTA GAAACACAGT ACATGGATGG AGTAGTAAAA
    ACCAAAGCTG ATGATTGCCA GTTTGTGTGC ATAGCACAAC AGGACTACTT TCGGATATTA
    AACCATGTGG AAAAAAATAC CCACAAAGTC AAGGAAAAGG GGGAGATTGT TATGGTTAAT
    GAACACCGAG AACTGGATCG AAGTGGCACT CGAAAGGGAC ATATAGTAAT TCAGGGGACT
    CCAGAAAGAC TCATCTTGCA CCTAATAGAA GAGCACTCTG TTGTTGACCC AACATACATA
    GAGGATTTTC TATTAACATA CAGGACATTC CTTTCAAGTC CATTAGAGGT TGGGGACAAG
    CTTTTGGAGT GGTTTAAAGA TGACAGTCGT CGTGACAAGG TAACACGAGT TGTCCTTCTT
    TGGGTTAACA ACCACTTTAA TGATTTTGAA GGAGACCCTG CCATGACAAG GTTTTTGGAA
    GAATTTGAAC GGAATCTTGA AAATGCGAAA ATGAAGGGTC ATCTGCGACT GCTAAACATA
    GCCTGTGCTG CAAAGGCTAA ACTGAGACAG GTTACATTGC TTAAGCCTTC CCGTGAATCG
    CCTTTTTATT TCAACGTCGT TGGGGGAAGC GACAAAGGTT TCGGCATGTT TATTGAAAGT
    GTTGAGCCTG GAAGCAAAGC AACAGAAGCT GGATTAAAAC GCGGAGATCA GATTATGGAA
    GTCAATGGCC AAAATTTTGA GACTATCGTT TACGCCAAAG CCCTGGAGAT CTTGAAAAAC
    AATACACATC TTTCCATCAC TGTCAAGACC AACATTTTTG TGTTTAAAGA GCTTCTTAAT
    CGAATCGGGC AGGAGAAAAA AAATGGAGTC CCACACATCC CCAAAATTGC AGAGAAGAAG
    AACAATAGAT ATTCCATCCC TGATCTCCCC AATGATGTAG AACAGATGTT TCCACGGGAT
    CGCGCAAGCA AGAAAATGAA AGCCAATACG GTGTCTGGAG GGCGGAATCG CATCCGGAAG
    ATTTTGGACA AAACACGTTT TAGTATATTA CCACCTAAGC CATTCAGTGA TGGCCATGTT
    ATCCAGTCCC AGGATGACAG TATTGTTGGC ACCAGGCAGT GCAGGCACAG TGTGGCTATA
    ATGCCCATTC CGGGCACGCT CTCTTCAAGC AGTCCAGACT TGCTTCAAGC TACAACAAAC
    ATATTGGATT TCTCCAATCC TACTGCTTTT GGATTTTACT ATATTCCAGA CCAAGTTATA
    CGAATATTTA AAGCAGATCA GCAGTGCTGC TATATTATCA TCGGCAAAGA CACTACTGCA
    AAGGAGGTGG TGATACAAGC ACTTCATGAA TTCAGCCTTA CTGGGTCAAC AGACACATAC
    TCTCTCTGTG AGGTCTCGGT CAGTCCAGAA GGTGTCATTA AACAAAGACG GCTTCCGGAT
    CAGCTTTCTA AACTGGCAGA CAGAATCCAG CTGAATGGAA GGTATTATTT GAAGAACAAC
    ATGGAGACCG AAACGTTGTG TTCAGATGAA GATGCTCAGG AGCTTCTGCG TGAAAGCCTC
    ATCTCTCTCC TTCAGCTTAG CACTATCGAG GTGGCAACAC AGCTTTCTAT GCGGGACTTT
    GAACTCTTCC GCAATATTGA GCCCACAGAA TACATAGATG ACCTTTTCAA GATAGATTCC
    AAGACAAGTG CTGCTCATCT GAAACAGTTT GAGGAAGTGA TCAACCAAGA GACATTTTGG
    GTGGCCACGG AAATTCTAAG AGAACCAAAC CATTTGAAGA GAATGAAAAT TGTGAAGCAT
    TTCATAAAGA CTGCCCTCCA TTGCAGAGAA TGCAGAAATT TTAACTCCAT GTTTGCTATA
    ATAAGTGGGC TCAATCTTGC ACCTGTGGCT CGGCTTCGAG GAACTTGGGA AAAGTTGCCC
    AGCAAATACG AGAAGCTGCT TCGAGACCTG CAAGATCTCT TCGACCCCTC AAGAAATATG
    GCGAAATACC GTAATATTCT AAGCAGTCAG AGCATGCAGC CCCCCATCAT TCCTCTCTTC
    CCAGTCGTCA AGAAGGATAT AACATTTCTT CATGAAGGAA ATGATTCAAA AGTTGATGGG
    TTAGTAAATT TTGAGAAGCT GCGAATGATT GCAAAGGAGA TTCGACATGT TATTCGCATG
    ACTTCTGCTA ATATGGATCC AGCAGTTATG TTTAGGCAAA GGAAGAAGAG ATGGAGAAGT
    TTAGGGTCCC TAAGCCAAGG CAGCACAAAC TCTAACATGC TGGATGTTCA AGGAGGTGGT
    CACAAGAAAC GGGCTCGGCG AAGTTCATTC CTTAATGCTA AGAAGCTGTA TGAAGATGCA
    CAGATGTCAA GGAAGGTAAA ACAGTACATC TCCAACCTGA GTGTGGAAAT CGATGAGGAG
    AAGATCCAGC TAATGTCACT GCAATGTGAA CCAGCCTACA GCACTTTGGC AAAGAATTTA
    AGTGAGAGAA GATCTGCAAA ATCTGACTTG TCTCCTGCAC CTTTGAGGCC ATCAGGCCAA
    ACTACCAAAG CTCACATCCA TCAGCAAAAC AGAATGAGCC AAGTATTGCA AGTCCCTACA
    GTTAACTTAT ATCCAATGCG AAAGAAAGCA CCAACCAAGG AACATCTTCC ATGCACTTCA
    GGATCTTCAC AGAAAATATC TGGTTTGACA GATGAGGTTA CAGTTAAAGC ACAGTTAGAA
    GACTCTGTTT CTCTTGTGTC ATCATTGCAT TCCAGCCCAG CCGCATCCCC TCAAGGATCT
    CCACATAAAG TTGTTAACAT TTCAAGATCA GAGAATTCCA GTCATATGAA CCTCTCTGGA
    TCCTCCTCAT CATTAACCAG TGATCCCAAC CAGAAGATCT GCAGTCGGCG TGGCTATGGA
    ATAGGCTATG CCCATAACCC CTCGGCCAAA TCTGATAACT TCTCAGACTC AAGCAACAGT
    GAGATCTCGT CCCGATCAAG CCTCGTCAGC AACTGCTCAG TAGACTCCAT GTCGTTGACC
    CTGCAGGATG AAAAGTGTGT GCCTCATTCA CATGCTGAGT CAATGGGACT ATCAGAAAGA
    AAAGACGTCC ATCCGTCCTC TGAGTATAAC CAGCCCTGCA GTAGCTGGCT CCATGCAAGG
    CACCCTGCAG TCCGGAATTT CATAGCTTGC GCAAGCAGTG AGGACATAGC TCATGACCAC
    ATATCGGTAG AGCCGGTAGA CAGTGGTCGG GGCAGTTGGA CATCTTGTTC TAGCAGTTCA
    CATGACAATT TCCAGACTTT GCAGAAGGGA CTGGACCGCT TAAGCACTTA CCGCCAATCA
    CACATTGATT CCCCAATTGC TGAGACAGAG TCCGCAAACT GCAGCACAGA TGAAACATGT
    AGTGAAGACT GTACCAAAGG AAAAAGGCAC TCAAACAACA GTTTAGAACT AAATGAATCT
    CGGCAAAGTT GGGCTTCATC TAGCTCAATG TCAGATACTT ACGAGACGAA CTATGGCACA
    ATCAAGCGGA GAGTATTGGA CACAGAGGAA AAAACAGAGG CTTTGGGCCA ACAATTAGAT
    GTCTTTTATA AAACAGTTAC TTCCACTACT GAAAAAGGAC TGATAGAAGA TGAACAAATG
    TCAGCAGTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa43157
    Clone ID Related Accession (Same CDS sequence) XM_018092134.1 , XM_018092134.2
    Accession Version XM_018092134.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4632bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 6 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018092134.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTCTGCCA GCATTGAACC CAGGTTTAAA GAAGCCTTGA GGAAAAAACC CAAGGAAAGG 
    ACTCAGGAGG ATCTGAAAAC TGTTTATTCA TATATGCATA GAATGGATAT ACTCTCACAT
    CTCAGAGAAC ATCAATTAAG ATTGATGTCT GCAACAGCAC GATATGAAAG ATACAGTGCA
    AATCAGGTTC TATTCTGCTC AGAAAGTTTG GCTAGATGTT GGTACATTCT TCTTTCTGGT
    TCAGTTCTGG TAAAAAACTC CATGTTTTTG CCTCCATGCA GTTTTGGCAA GCAGTCAGGT
    GGCAAAAGGG GTTGTGATTG CATTGTACTG GAACCTTCTG AAATGATTGT GGTGGAAAAT
    ACAGGAGAAA ACAATGATGG CATTCTGCAT AGGGAAACTT CTATTAGACA ATCCCGGCGT
    CGGTATCGAA AAATCAACCA GCATGGAGAA CGCCAAACAA TTACAGATAA TGGGGATGCT
    GCTAGTTATG TCTTGCTCCC CACAGACCTC TCCAAGATAC ACCTCACCGA CAGTCCCCAC
    CCACATGTTA CACATGTGCC ATCCAGCCAA TCAGGATGCA GCATTGCAAG CGACTCTGGA
    AGCAGCAGCT TATCAGATAT CTACCAGGCA ACAGAAAGTG AGATGGGGGA TGTTGACTTG
    ACAGGCCTGC CAGAGGCAGC TGTTGATTCT GAAGATGAAG AGGAAGATGA AGATATTGAG
    AGAGCTTCAG ACACAATGCT GACCCGTGAC ATTGTGAGGG AATGTCTTGA GAAAGAACCC
    ACAGACAGAA CAGATGATGA CATAGAACAG CTACTGGAAT TCATGCATCA GCTTCCAGCC
    TTTGTCAATA TGACCATGTC TGTGAGACGA AAGCTTTGCT CGGTCATGAT TTTTGAAGTT
    GTGGAACAGG CTGGCGCAAT CATCATTGAA GATGGGCAAG AACTGGACTC TTGGTATGTC
    ATTCTTAATG GGATTGTAGA AATCAGCCAT TCAGATGGCA GGATGGAAAA CCTTTGCATG
    GGAAATAGCT TTGGCATAAA CCCATCTTTA GAAACACAGT ACATGGATGG AGTAGTAAAA
    ACCAAAGCTG ATGATTGCCA GTTTGTGTGC ATAGCACAAC AGGACTACTT TCGGATATTA
    AACCATGTGG AAAAAAATAC CCACAAAGTC AAGGAAAAGG GGGAGATTGT TATGGTTAAT
    GAACACCGAG AACTGGATCG AAGTGGCACT CGAAAGGGAC ATATAGTAAT TCAGGGGACT
    CCAGAAAGAC TCATCTTGCA CCTAATAGAA GAGCACTCTG TTGTTGACCC AACATACATA
    GAGGATTTTC TATTAACATA CAGGACATTC CTTTCAAGTC CATTAGAGGT TGGGGACAAG
    CTTTTGGAGT GGTTTAAAGA TGACAGTCGT CGTGACAAGG TAACACGAGT TGTCCTTCTT
    TGGGTTAACA ACCACTTTAA TGATTTTGAA GGAGACCCTG CCATGACAAG GTTTTTGGAA
    GAATTTGAAC GGAATCTTGA AAATGCGAAA ATGAAGGGTC ATCTGCGACT GCTAAACATA
    GCCTGTGCTG CAAAGGCTAA ACTGAGACAG GTTACATTGC TTAAGCCTTC CCGTGAATCG
    CCTTTTTATT TCAACGTCGT TGGGGGAAGC GACAAAGGTT TCGGCATGTT TATTGAAAGT
    GTTGAGCCTG GAAGCAAAGC AACAGAAGCT GGATTAAAAC GCGGAGATCA GATTATGGAA
    GTCAATGGCC AAAATTTTGA GACTATCGTT TACGCCAAAG CCCTGGAGAT CTTGAAAAAC
    AATACACATC TTTCCATCAC TGTCAAGACC AACATTTTTG TGTTTAAAGA GCTTCTTAAT
    CGAATCGGGC AGGAGAAAAA AAATGGAGTC CCACACATCC CCAAAATTGC AGAGAAGAAG
    AACAATAGAT ATTCCATCCC TGATCTCCCC AATGATGTAG AACAGATGTT TCCACGGGAT
    CGCGCAAGCA AGAAAATGAA AGCCAATACG GTGTCTGGAG GGCGGAATCG CATCCGGAAG
    ATTTTGGACA AAACACGTTT TAGTATATTA CCACCTAAGC CATTCAGTGA TGGCCATGTT
    ATCCAGTCCC AGGATGACAG TATTGTTGGC ACCAGGCAGT GCAGGCACAG TGTGGCTATA
    ATGCCCATTC CGGGCACGCT CTCTTCAAGC AGTCCAGACT TGCTTCAAGC TACAACAAAC
    ATATTGGATT TCTCCAATCC TACTGCTTTT GGATTTTACT ATATTCCAGA CCAAGTTATA
    CGAATATTTA AAGCAGATCA GCAGTGCTGC TATATTATCA TCGGCAAAGA CACTACTGCA
    AAGGAGGTGG TGATACAAGC ACTTCATGAA TTCAGCCTTA CTGGGTCAAC AGACACATAC
    TCTCTCTGTG AGGTCTCGGT CAGTCCAGAA GGTGTCATTA AACAAAGACG GCTTCCGGAT
    CAGCTTTCTA AACTGGCAGA CAGAATCCAG CTGAATGGAA GGTATTATTT GAAGAACAAC
    ATGGAGACCG AAACGTTGTG TTCAGATGAA GATGCTCAGG AGCTTCTGCG TGAAAGCCTC
    ATCTCTCTCC TTCAGCTTAG CACTATCGAG GTGGCAACAC AGCTTTCTAT GCGGGACTTT
    GAACTCTTCC GCAATATTGA GCCCACAGAA TACATAGATG ACCTTTTCAA GATAGATTCC
    AAGACAAGTG CTGCTCATCT GAAACAGTTT GAGGAAGTGA TCAACCAAGA GACATTTTGG
    GTGGCCACGG AAATTCTAAG AGAACCAAAC CATTTGAAGA GAATGAAAAT TGTGAAGCAT
    TTCATAAAGA CTGCCCTCCA TTGCAGAGAA TGCAGAAATT TTAACTCCAT GTTTGCTATA
    ATAAGTGGGC TCAATCTTGC ACCTGTGGCT CGGCTTCGAG GAACTTGGGA AAAGTTGCCC
    AGCAAATACG AGAAGCTGCT TCGAGACCTG CAAGATCTCT TCGACCCCTC AAGAAATATG
    GCGAAATACC GTAATATTCT AAGCAGTCAG AGCATGCAGC CCCCCATCAT TCCTCTCTTC
    CCAGTCGTCA AGAAGGATAT AACATTTCTT CATGAAGGAA ATGATTCAAA AGTTGATGGG
    TTAGTAAATT TTGAGAAGCT GCGAATGATT GCAAAGGAGA TTCGACATGT TATTCGCATG
    ACTTCTGCTA ATATGGATCC AGCAGTTATG TTTAGGCAAA GGAAGAAGAG ATGGAGAAGT
    TTAGGGTCCC TAAGCCAAGG CAGCACAAAC TCTAACATGC TGGATGTTCA AGGAGGTGGT
    CACAAGAAAC GGGCTCGGCG AAGTTCATTC CTTAATGCTA AGAAGCTGTA TGAAGATGCA
    CAGATGTCAA GGAAGGTAAA ACAGTACATC TCCAACCTGA GTGTGGAAAT CGATGAGGAG
    AAGATCCAGC TAATGTCACT GCAATGTGAA CCAGCCTACA GCACTTTGGC AAAGAATTTA
    AGTGAGAGAA GATCTGCAAA ATCTGACTTG TCTCCTGCAC CTTTGAGGCC ATCAGGCCAA
    ACTACCAAAG CTCACATCCA TCAGCAAAAC AGAATGAGCC AAGTATTGCA AGTCCCTACA
    GTTAACTTAT ATCCAATGCG AAAGAAAGCA CCAACCAAGG AACATCTTCC ATGCACTTCA
    GGATCTTCAC AGAAAATATC TGGTTTGACA GATGAGGTTA CAGTTAAAGC ACAGTTAGAA
    GACTCTGTTT CTCTTGTGTC ATCATTGCAT TCCAGCCCAG CCGCATCCCC TCAAGGATCT
    CCACATAAAG TTGTTAACAT TTCAAGATCA GAGAATTCCA GTCATATGAA CCTCTCTGGA
    TCCTCCTCAT CATTAACCAG TGATCCCAAC CAGAAGATCT GCAGTCGGCG TGGCTATGGA
    ATAGGCTATG CCCATAACCC CTCGGCCAAA TCTGATAACT TCTCAGACTC AAGCAACAGT
    GAGATCTCGT CCCGATCAAG CCTCGTCAGC AACTGCTCAG TAGACTCCAT GTCGTTGACC
    CTGCAGGATG AAAAGTGTGT GCCTCATTCA CATGCTGAGT CAATGGGACT ATCAGAAAGA
    AAAGACGTCC ATCCGTCCTC TGAGTATAAC CAGCCCTGCA GTAGCTGGCT CCATGCAAGG
    CACCCTGCAG TCCGGAATTT CATAGCTTGC GCAAGCAGTG AGGACATAGC TCATGACCAC
    ATATCGGTAG AGCCGGTAGA CAGTGGTCGG GGCAGTTGGA CATCTTGTTC TAGCAGTTCA
    CATGACAATT TCCAGACTTT GCAGAAGGGA CTGGACCGCT TAAGCACTTA CCGCCAATCA
    CACATTGATT CCCCAATTGC TGAGACAGAG TCCGCAAACT GCAGCACAGA TGAAACATGT
    AGTGAAGACT GTACCAAAGG AAAAAGGCAC TCAAACAACA GTTTAGAACT AAATGAATCT
    CGGCAAAGTT GGGCTTCATC TAGCTCAATG TCAGATACTT ACGAGACGAA CTATGGCACA
    ATCAAGCGGA GAGTATTGGA CACAGAGGAA AAAACAGAGG CTTTGGGCCA ACAATTAGAT
    GTCTTTTATA AAACAGTTAC TTCCACTACT GAAAAAGGAC TGATAGAAGA TGAACAAATG
    TCAGCAGTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947623.1
    CDS487..5118
    Translation

    Target ORF information:

    RefSeq Version XM_018092134.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 6 (rapgef6), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092134.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTCTGCCA GCATTGAACC CAGGTTTAAA GAAGCCTTGA GGAAAAAACC CAAGGAAAGG 
    ACTCAGGAGG ATCTGAAAAC TGTTTATTCA TATATGCATA GAATGGATAT ACTCTCACAT
    CTCAGAGAAC ATCAATTAAG ATTGATGTCT GCAACAGCAC GATATGAAAG ATACAGTGCA
    AATCAGGTTC TATTCTGCTC AGAAAGTTTG GCTAGATGTT GGTACATTCT TCTTTCTGGT
    TCAGTTCTGG TAAAAAACTC CATGTTTTTG CCTCCATGCA GTTTTGGCAA GCAGTCAGGT
    GGCAAAAGGG GTTGTGATTG CATTGTACTG GAACCTTCTG AAATGATTGT GGTGGAAAAT
    ACAGGAGAAA ACAATGATGG CATTCTGCAT AGGGAAACTT CTATTAGACA ATCCCGGCGT
    CGGTATCGAA AAATCAACCA GCATGGAGAA CGCCAAACAA TTACAGATAA TGGGGATGCT
    GCTAGTTATG TCTTGCTCCC CACAGACCTC TCCAAGATAC ACCTCACCGA CAGTCCCCAC
    CCACATGTTA CACATGTGCC ATCCAGCCAA TCAGGATGCA GCATTGCAAG CGACTCTGGA
    AGCAGCAGCT TATCAGATAT CTACCAGGCA ACAGAAAGTG AGATGGGGGA TGTTGACTTG
    ACAGGCCTGC CAGAGGCAGC TGTTGATTCT GAAGATGAAG AGGAAGATGA AGATATTGAG
    AGAGCTTCAG ACACAATGCT GACCCGTGAC ATTGTGAGGG AATGTCTTGA GAAAGAACCC
    ACAGACAGAA CAGATGATGA CATAGAACAG CTACTGGAAT TCATGCATCA GCTTCCAGCC
    TTTGTCAATA TGACCATGTC TGTGAGACGA AAGCTTTGCT CGGTCATGAT TTTTGAAGTT
    GTGGAACAGG CTGGCGCAAT CATCATTGAA GATGGGCAAG AACTGGACTC TTGGTATGTC
    ATTCTTAATG GGATTGTAGA AATCAGCCAT TCAGATGGCA GGATGGAAAA CCTTTGCATG
    GGAAATAGCT TTGGCATAAA CCCATCTTTA GAAACACAGT ACATGGATGG AGTAGTAAAA
    ACCAAAGCTG ATGATTGCCA GTTTGTGTGC ATAGCACAAC AGGACTACTT TCGGATATTA
    AACCATGTGG AAAAAAATAC CCACAAAGTC AAGGAAAAGG GGGAGATTGT TATGGTTAAT
    GAACACCGAG AACTGGATCG AAGTGGCACT CGAAAGGGAC ATATAGTAAT TCAGGGGACT
    CCAGAAAGAC TCATCTTGCA CCTAATAGAA GAGCACTCTG TTGTTGACCC AACATACATA
    GAGGATTTTC TATTAACATA CAGGACATTC CTTTCAAGTC CATTAGAGGT TGGGGACAAG
    CTTTTGGAGT GGTTTAAAGA TGACAGTCGT CGTGACAAGG TAACACGAGT TGTCCTTCTT
    TGGGTTAACA ACCACTTTAA TGATTTTGAA GGAGACCCTG CCATGACAAG GTTTTTGGAA
    GAATTTGAAC GGAATCTTGA AAATGCGAAA ATGAAGGGTC ATCTGCGACT GCTAAACATA
    GCCTGTGCTG CAAAGGCTAA ACTGAGACAG GTTACATTGC TTAAGCCTTC CCGTGAATCG
    CCTTTTTATT TCAACGTCGT TGGGGGAAGC GACAAAGGTT TCGGCATGTT TATTGAAAGT
    GTTGAGCCTG GAAGCAAAGC AACAGAAGCT GGATTAAAAC GCGGAGATCA GATTATGGAA
    GTCAATGGCC AAAATTTTGA GACTATCGTT TACGCCAAAG CCCTGGAGAT CTTGAAAAAC
    AATACACATC TTTCCATCAC TGTCAAGACC AACATTTTTG TGTTTAAAGA GCTTCTTAAT
    CGAATCGGGC AGGAGAAAAA AAATGGAGTC CCACACATCC CCAAAATTGC AGAGAAGAAG
    AACAATAGAT ATTCCATCCC TGATCTCCCC AATGATGTAG AACAGATGTT TCCACGGGAT
    CGCGCAAGCA AGAAAATGAA AGCCAATACG GTGTCTGGAG GGCGGAATCG CATCCGGAAG
    ATTTTGGACA AAACACGTTT TAGTATATTA CCACCTAAGC CATTCAGTGA TGGCCATGTT
    ATCCAGTCCC AGGATGACAG TATTGTTGGC ACCAGGCAGT GCAGGCACAG TGTGGCTATA
    ATGCCCATTC CGGGCACGCT CTCTTCAAGC AGTCCAGACT TGCTTCAAGC TACAACAAAC
    ATATTGGATT TCTCCAATCC TACTGCTTTT GGATTTTACT ATATTCCAGA CCAAGTTATA
    CGAATATTTA AAGCAGATCA GCAGTGCTGC TATATTATCA TCGGCAAAGA CACTACTGCA
    AAGGAGGTGG TGATACAAGC ACTTCATGAA TTCAGCCTTA CTGGGTCAAC AGACACATAC
    TCTCTCTGTG AGGTCTCGGT CAGTCCAGAA GGTGTCATTA AACAAAGACG GCTTCCGGAT
    CAGCTTTCTA AACTGGCAGA CAGAATCCAG CTGAATGGAA GGTATTATTT GAAGAACAAC
    ATGGAGACCG AAACGTTGTG TTCAGATGAA GATGCTCAGG AGCTTCTGCG TGAAAGCCTC
    ATCTCTCTCC TTCAGCTTAG CACTATCGAG GTGGCAACAC AGCTTTCTAT GCGGGACTTT
    GAACTCTTCC GCAATATTGA GCCCACAGAA TACATAGATG ACCTTTTCAA GATAGATTCC
    AAGACAAGTG CTGCTCATCT GAAACAGTTT GAGGAAGTGA TCAACCAAGA GACATTTTGG
    GTGGCCACGG AAATTCTAAG AGAACCAAAC CATTTGAAGA GAATGAAAAT TGTGAAGCAT
    TTCATAAAGA CTGCCCTCCA TTGCAGAGAA TGCAGAAATT TTAACTCCAT GTTTGCTATA
    ATAAGTGGGC TCAATCTTGC ACCTGTGGCT CGGCTTCGAG GAACTTGGGA AAAGTTGCCC
    AGCAAATACG AGAAGCTGCT TCGAGACCTG CAAGATCTCT TCGACCCCTC AAGAAATATG
    GCGAAATACC GTAATATTCT AAGCAGTCAG AGCATGCAGC CCCCCATCAT TCCTCTCTTC
    CCAGTCGTCA AGAAGGATAT AACATTTCTT CATGAAGGAA ATGATTCAAA AGTTGATGGG
    TTAGTAAATT TTGAGAAGCT GCGAATGATT GCAAAGGAGA TTCGACATGT TATTCGCATG
    ACTTCTGCTA ATATGGATCC AGCAGTTATG TTTAGGCAAA GGAAGAAGAG ATGGAGAAGT
    TTAGGGTCCC TAAGCCAAGG CAGCACAAAC TCTAACATGC TGGATGTTCA AGGAGGTGGT
    CACAAGAAAC GGGCTCGGCG AAGTTCATTC CTTAATGCTA AGAAGCTGTA TGAAGATGCA
    CAGATGTCAA GGAAGGTAAA ACAGTACATC TCCAACCTGA GTGTGGAAAT CGATGAGGAG
    AAGATCCAGC TAATGTCACT GCAATGTGAA CCAGCCTACA GCACTTTGGC AAAGAATTTA
    AGTGAGAGAA GATCTGCAAA ATCTGACTTG TCTCCTGCAC CTTTGAGGCC ATCAGGCCAA
    ACTACCAAAG CTCACATCCA TCAGCAAAAC AGAATGAGCC AAGTATTGCA AGTCCCTACA
    GTTAACTTAT ATCCAATGCG AAAGAAAGCA CCAACCAAGG AACATCTTCC ATGCACTTCA
    GGATCTTCAC AGAAAATATC TGGTTTGACA GATGAGGTTA CAGTTAAAGC ACAGTTAGAA
    GACTCTGTTT CTCTTGTGTC ATCATTGCAT TCCAGCCCAG CCGCATCCCC TCAAGGATCT
    CCACATAAAG TTGTTAACAT TTCAAGATCA GAGAATTCCA GTCATATGAA CCTCTCTGGA
    TCCTCCTCAT CATTAACCAG TGATCCCAAC CAGAAGATCT GCAGTCGGCG TGGCTATGGA
    ATAGGCTATG CCCATAACCC CTCGGCCAAA TCTGATAACT TCTCAGACTC AAGCAACAGT
    GAGATCTCGT CCCGATCAAG CCTCGTCAGC AACTGCTCAG TAGACTCCAT GTCGTTGACC
    CTGCAGGATG AAAAGTGTGT GCCTCATTCA CATGCTGAGT CAATGGGACT ATCAGAAAGA
    AAAGACGTCC ATCCGTCCTC TGAGTATAAC CAGCCCTGCA GTAGCTGGCT CCATGCAAGG
    CACCCTGCAG TCCGGAATTT CATAGCTTGC GCAAGCAGTG AGGACATAGC TCATGACCAC
    ATATCGGTAG AGCCGGTAGA CAGTGGTCGG GGCAGTTGGA CATCTTGTTC TAGCAGTTCA
    CATGACAATT TCCAGACTTT GCAGAAGGGA CTGGACCGCT TAAGCACTTA CCGCCAATCA
    CACATTGATT CCCCAATTGC TGAGACAGAG TCCGCAAACT GCAGCACAGA TGAAACATGT
    AGTGAAGACT GTACCAAAGG AAAAAGGCAC TCAAACAACA GTTTAGAACT AAATGAATCT
    CGGCAAAGTT GGGCTTCATC TAGCTCAATG TCAGATACTT ACGAGACGAA CTATGGCACA
    ATCAAGCGGA GAGTATTGGA CACAGAGGAA AAAACAGAGG CTTTGGGCCA ACAATTAGAT
    GTCTTTTATA AAACAGTTAC TTCCACTACT GAAAAAGGAC TGATAGAAGA TGAACAAATG
    TCAGCAGTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa43158
    Clone ID Related Accession (Same CDS sequence) XM_018092135.1
    Accession Version XM_018092135.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4593bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 6 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGATTGTGG TGGAAAATAC AGGAGAAAAC AATGATGGCA TTCTGCATAG GGAAACTTCT 
    ATTAGACAAT CCCGGCGTCG GTATCGAAAA ATCAACCAGC ATGGAGAACG CCAAACAATT
    ACAGATAATG GGGATGCTGC TAGTTATGTC TTGCTCCCCA CAGACCTCTC CAAGATACAC
    CTCACCGACA GTCCCCACCC ACATGTTACA CATGTGCCAT CCAGCCAATC AGGATGCAGC
    ATTGCAAGCG ACTCTGGAAG CAGCAGCTTA TCAGATATCT ACCAGGCAAC AGAAAGTGAG
    ATGGGGGATG TTGACTTGAC AGGCCTGCCA GAGGCAGCTG TTGATTCTGA AGATGAAGAG
    GAAGATGAAG ATATTGAGAG AGCTTCAGAC ACAATGCTGA CCCGTGACAT TGTGAGGGAA
    TGTCTTGAGA AAGAACCCAC AGACAGAACA GATGATGACA TAGAACAGCT ACTGGAATTC
    ATGCATCAGC TTCCAGCCTT TGTCAATATG ACCATGTCTG TGAGACGAAA GCTTTGCTCG
    GTCATGATTT TTGAAGTTGT GGAACAGGCT GGCGCAATCA TCATTGAAGA TGGGCAAGAA
    CTGGACTCTT GGTATGTCAT TCTTAATGGG ATTGTAGAAA TCAGCCATTC AGATGGCAGG
    ATGGAAAACC TTTGCATGGG AAATAGCTTT GGCATAAACC CATCTTTAGA AACACAGTAC
    ATGGATGGAG TAGTAAAAAC CAAAGCTGAT GATTGCCAGT TTGTGTGCAT AGCACAACAG
    GACTACTTTC GGATATTAAA CCATGTGGAA AAAAATACCC ACAAAGTCAA GGAAAAGGGG
    GAGATTGTTA TGGTTAATGA ACACCGAGAA CTGGATCGAA GTGGCACTCG AAAGGGACAT
    ATAGTAATTC AGGGGACTCC AGAAAGACTC ATCTTGCACC TAATAGAAGA GCACTCTGTT
    GTTGACCCAA CATACATAGA GGATTTTCTA TTAACATACA GGACATTCCT TTCAAGTCCA
    TTAGAGGTTG GGGACAAGCT TTTGGAGTGG TTTAAAGATG ACAGTCGTCG TGACAAGGTA
    ACACGAGTTG TCCTTCTTTG GGTTAACAAC CACTTTAATG ATTTTGAAGG AGACCCTGCC
    ATGACAAGGT TTTTGGAAGA ATTTGAACGG AATCTTGAAA ATGCGAAAAT GAAGGGTCAT
    CTGCGACTGC TAAACATAGC CTGTGCTGCA AAGGCTAAAC TGAGACAGGT TACATTGCTT
    AAGCCTTCCC GTGAATCGCC TTTTTATTTC AACGTCGTTG GGGGAAGCGA CAAAGGTTTC
    GGCATGTTTA TTGAAAGTGT TGAGCCTGGA AGCAAAGCAA CAGAAGCTGG ATTAAAACGC
    GGAGATCAGA TTATGGAAGT CAATGGCCAA AATTTTGAGA CTATCGTTTA CGCCAAAGCC
    CTGGAGATCT TGAAAAACAA TACACATCTT TCCATCACTG TCAAGACCAA CATTTTTGTG
    TTTAAAGAGC TTCTTAATCG AATCGGGCAG GAGAAAAAAA ATGGAGTCCC ACACATCCCC
    AAAATTGCAG AGAAGAAGAA CAATAGATAT TCCATCCCTG ATCTCCCCAA TGATGTAGAA
    CAGATGTTTC CACGGGATCG CGCAAGCAAG AAAATGAAAG CCAATACGGT GTCTGGAGGG
    CGGAATCGCA TCCGGAAGAT TTTGGACAAA ACACGTTTTA GTATATTACC ACCTAAGCCA
    TTCAGTGATG GCCATGTTAT CCAGTCCCAG GATGACAGTA TTGTTGGCAC CAGGCAGTGC
    AGGCACAGTG TGGCTATAAT GCCCATTCCG GGCACGCTCT CTTCAAGCAG TCCAGACTTG
    CTTCAAGCTA CAACAAACAT ATTGGATTTC TCCAATCCTA CTGCTTTTGG ATTTTACTAT
    ATTCCAGACC AAGTTATACG AATATTTAAA GCAGATCAGC AGTGCTGCTA TATTATCATC
    GGCAAAGACA CTACTGCAAA GGAGGTGGTG ATACAAGCAC TTCATGAATT CAGCCTTACT
    GGGTCAACAG ACACATACTC TCTCTGTGAG GTCTCGGTCA GTCCAGAAGG TGTCATTAAA
    CAAAGACGGC TTCCGGATCA GCTTTCTAAA CTGGCAGACA GAATCCAGCT GAATGGAAGG
    TATTATTTGA AGAACAACAT GGAGACCGAA ACGTTGTGTT CAGATGAAGA TGCTCAGGAG
    CTTCTGCGTG AAAGCCTCAT CTCTCTCCTT CAGCTTAGCA CTATCGAGGT GGCAACACAG
    CTTTCTATGC GGGACTTTGA ACTCTTCCGC AATATTGAGC CCACAGAATA CATAGATGAC
    CTTTTCAAGA TAGATTCCAA GACAAGTGCT GCTCATCTGA AACAGTTTGA GGAAGTGATC
    AACCAAGAGA CATTTTGGGT GGCCACGGAA ATTCTAAGAG AACCAAACCA TTTGAAGAGA
    ATGAAAATTG TGAAGCATTT CATAAAGACT GCCCTCCATT GCAGAGAATG CAGAAATTTT
    AACTCCATGT TTGCTATAAT AAGTGGGCTC AATCTTGCAC CTGTGGCTCG GCTTCGAGGA
    ACTTGGGAAA AGTTGCCCAG CAAATACGAG AAGCTGCTTC GAGACCTGCA AGATCTCTTC
    GACCCCTCAA GAAATATGGC GAAATACCGT AATATTCTAA GCAGTCAGAG CATGCAGCCC
    CCCATCATTC CTCTCTTCCC AGTCGTCAAG AAGGATATAA CATTTCTTCA TGAAGGAAAT
    GATTCAAAAG TTGATGGGTT AGTAAATTTT GAGAAGCTGC GAATGATTGC AAAGGAGATT
    CGACATGTTA TTCGCATGAC TTCTGCTAAT ATGGATCCAG CAGTTATGTT TAGGCAAAGG
    AAGAAGAGAT GGAGAAGTTT AGGGTCCCTA AGCCAAGGCA GCACAAACTC TAACATGCTG
    GATGTTCAAG GAGGTGGTCA CAAGAAACGG GCTCGGCGAA GTTCATTCCT TAATGCTAAG
    AAGCTGTATG AAGATGCACA GATGTCAAGG AAGGTAAAAC AGTACATCTC CAACCTGAGT
    GTGGAAATCG ATGAGGAGAA GATCCAGCTA ATGTCACTGC AATGTGAACC AGCCTACAGC
    ACTTTGGCAA AGAATTTAAG TGAGAGAAGA TCTGCAAAAT CTGACTTGTC TCCTGCACCT
    TTGAGGCCAT CAGGCCAAAC TACCAAAGCT CACATCCATC AGCAAAACAG AATGAGCCAA
    GTATTGCAAG TCCCTACAGT TAACTTATAT CCAATGCGAA AGAAAGCACC AACCAAGGAA
    CATCTTCCAT GCACTTCAGG ATCTTCACAG AAAATATCTG GTTTGACAGA TGAGGTTACA
    GTTAAAGCAC AGTTAGAAGA CTCTGTTTCT CTTGTGTCAT CATTGCATTC CAGCCCAGCC
    GCATCCCCTC AAGGATCTCC ACATAAAGTT GTTAACATTT CAAGATCAGA GAATTCCAGT
    CATATGAACC TCTCTGGATC CTCCTCATCA TTAACCAGTG ATCCCAACCA GAAGATCTGC
    AGTCGGCGTG GCTATGGAAT AGGCTATGCC CATAACCCCT CGGCCAAATC TGATAACTTC
    TCAGACTCAA GCAACAGTGA GATCTCGTCC CGATCAAGCC TCGTCAGCAA CTGCTCAGTA
    GACTCCATGT CGTTGACCCT GCAGGATGAA AAGTGTGTGC CTCATTCACA TGCTGAGTCA
    ATGGGACTAT CAGAAAGAAA AGACGTCCAT CCGTCCTCTG AGTATAACCA GCCCTGCAGT
    AGCTGGCTCC ATGCAAGGCA CCCTGCAGTC CGGAATTTCA TAGCTTGCGC AAGCAGTGAG
    GACATAGCTC ATGACCACAT ATCGGTAGAG CCGGTAGACA GTGGTCGGGG CAGTTGGACA
    TCTTGTTCTA GCAGTTCACA TGACAATTTC CAGACTTTGC AGAAGGGACT GGACCGCTTA
    AGCACTTACC GCCAATCACA CATTGATTCC CCAATTGCTG AGACAGAGTC CGCAAACTGC
    AGCACAGATG AAACATGTAG TGAAGACTGT ACCAAAGGAA AAAGGCACTC AAACAACAGT
    TTAGAACTAA ATGAATCTCG GCAAAGTTGG GCTTCATCTA GCTCAATGTC AGATACTTAC
    GAGACGAACT ATGGCACAAT CAAGCGGAGA GTATTGGACA CAGAGGAAAA AACAGAGGCT
    TTGGGCCAAC AATTAGATGT CTTTTATAAA ACAGTTACTT CCACTACTGA AAAAGGACTG
    ATAGTGTACT GTGTCACCTC ATCCCAGAAG GACAGTAGGT ATAGGGAGCC ACCTCCCACT
    CCTCCAGGCT ACCAGGGGAT ATCATTAGCC GACATAAAGG AAGGACAACC CCACCAAGCG
    CACTTAAAAC CTCCAGATTA TAGTGTGGCG ATACAGAGGT CAAAGATGAT ACATAAAGAC
    CTCTCTAGGC TTTCATCAGC CTGTGTTGAA CCAGTCTCAT GTGTCTCACA GAAGATGATA
    ACCCATTGGC ATAACCGGCA TCAGCCTTAT CCTAATGTAC AGGCTGACAG AGATAGTAAA
    GAAGATGAAG ATGAACAAAT GTCAGCAGTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947624.1
    CDS517..5109
    Translation

    Target ORF information:

    RefSeq Version XM_018092135.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 6 (rapgef6), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092135.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGATTGTGG TGGAAAATAC AGGAGAAAAC AATGATGGCA TTCTGCATAG GGAAACTTCT 
    ATTAGACAAT CCCGGCGTCG GTATCGAAAA ATCAACCAGC ATGGAGAACG CCAAACAATT
    ACAGATAATG GGGATGCTGC TAGTTATGTC TTGCTCCCCA CAGACCTCTC CAAGATACAC
    CTCACCGACA GTCCCCACCC ACATGTTACA CATGTGCCAT CCAGCCAATC AGGATGCAGC
    ATTGCAAGCG ACTCTGGAAG CAGCAGCTTA TCAGATATCT ACCAGGCAAC AGAAAGTGAG
    ATGGGGGATG TTGACTTGAC AGGCCTGCCA GAGGCAGCTG TTGATTCTGA AGATGAAGAG
    GAAGATGAAG ATATTGAGAG AGCTTCAGAC ACAATGCTGA CCCGTGACAT TGTGAGGGAA
    TGTCTTGAGA AAGAACCCAC AGACAGAACA GATGATGACA TAGAACAGCT ACTGGAATTC
    ATGCATCAGC TTCCAGCCTT TGTCAATATG ACCATGTCTG TGAGACGAAA GCTTTGCTCG
    GTCATGATTT TTGAAGTTGT GGAACAGGCT GGCGCAATCA TCATTGAAGA TGGGCAAGAA
    CTGGACTCTT GGTATGTCAT TCTTAATGGG ATTGTAGAAA TCAGCCATTC AGATGGCAGG
    ATGGAAAACC TTTGCATGGG AAATAGCTTT GGCATAAACC CATCTTTAGA AACACAGTAC
    ATGGATGGAG TAGTAAAAAC CAAAGCTGAT GATTGCCAGT TTGTGTGCAT AGCACAACAG
    GACTACTTTC GGATATTAAA CCATGTGGAA AAAAATACCC ACAAAGTCAA GGAAAAGGGG
    GAGATTGTTA TGGTTAATGA ACACCGAGAA CTGGATCGAA GTGGCACTCG AAAGGGACAT
    ATAGTAATTC AGGGGACTCC AGAAAGACTC ATCTTGCACC TAATAGAAGA GCACTCTGTT
    GTTGACCCAA CATACATAGA GGATTTTCTA TTAACATACA GGACATTCCT TTCAAGTCCA
    TTAGAGGTTG GGGACAAGCT TTTGGAGTGG TTTAAAGATG ACAGTCGTCG TGACAAGGTA
    ACACGAGTTG TCCTTCTTTG GGTTAACAAC CACTTTAATG ATTTTGAAGG AGACCCTGCC
    ATGACAAGGT TTTTGGAAGA ATTTGAACGG AATCTTGAAA ATGCGAAAAT GAAGGGTCAT
    CTGCGACTGC TAAACATAGC CTGTGCTGCA AAGGCTAAAC TGAGACAGGT TACATTGCTT
    AAGCCTTCCC GTGAATCGCC TTTTTATTTC AACGTCGTTG GGGGAAGCGA CAAAGGTTTC
    GGCATGTTTA TTGAAAGTGT TGAGCCTGGA AGCAAAGCAA CAGAAGCTGG ATTAAAACGC
    GGAGATCAGA TTATGGAAGT CAATGGCCAA AATTTTGAGA CTATCGTTTA CGCCAAAGCC
    CTGGAGATCT TGAAAAACAA TACACATCTT TCCATCACTG TCAAGACCAA CATTTTTGTG
    TTTAAAGAGC TTCTTAATCG AATCGGGCAG GAGAAAAAAA ATGGAGTCCC ACACATCCCC
    AAAATTGCAG AGAAGAAGAA CAATAGATAT TCCATCCCTG ATCTCCCCAA TGATGTAGAA
    CAGATGTTTC CACGGGATCG CGCAAGCAAG AAAATGAAAG CCAATACGGT GTCTGGAGGG
    CGGAATCGCA TCCGGAAGAT TTTGGACAAA ACACGTTTTA GTATATTACC ACCTAAGCCA
    TTCAGTGATG GCCATGTTAT CCAGTCCCAG GATGACAGTA TTGTTGGCAC CAGGCAGTGC
    AGGCACAGTG TGGCTATAAT GCCCATTCCG GGCACGCTCT CTTCAAGCAG TCCAGACTTG
    CTTCAAGCTA CAACAAACAT ATTGGATTTC TCCAATCCTA CTGCTTTTGG ATTTTACTAT
    ATTCCAGACC AAGTTATACG AATATTTAAA GCAGATCAGC AGTGCTGCTA TATTATCATC
    GGCAAAGACA CTACTGCAAA GGAGGTGGTG ATACAAGCAC TTCATGAATT CAGCCTTACT
    GGGTCAACAG ACACATACTC TCTCTGTGAG GTCTCGGTCA GTCCAGAAGG TGTCATTAAA
    CAAAGACGGC TTCCGGATCA GCTTTCTAAA CTGGCAGACA GAATCCAGCT GAATGGAAGG
    TATTATTTGA AGAACAACAT GGAGACCGAA ACGTTGTGTT CAGATGAAGA TGCTCAGGAG
    CTTCTGCGTG AAAGCCTCAT CTCTCTCCTT CAGCTTAGCA CTATCGAGGT GGCAACACAG
    CTTTCTATGC GGGACTTTGA ACTCTTCCGC AATATTGAGC CCACAGAATA CATAGATGAC
    CTTTTCAAGA TAGATTCCAA GACAAGTGCT GCTCATCTGA AACAGTTTGA GGAAGTGATC
    AACCAAGAGA CATTTTGGGT GGCCACGGAA ATTCTAAGAG AACCAAACCA TTTGAAGAGA
    ATGAAAATTG TGAAGCATTT CATAAAGACT GCCCTCCATT GCAGAGAATG CAGAAATTTT
    AACTCCATGT TTGCTATAAT AAGTGGGCTC AATCTTGCAC CTGTGGCTCG GCTTCGAGGA
    ACTTGGGAAA AGTTGCCCAG CAAATACGAG AAGCTGCTTC GAGACCTGCA AGATCTCTTC
    GACCCCTCAA GAAATATGGC GAAATACCGT AATATTCTAA GCAGTCAGAG CATGCAGCCC
    CCCATCATTC CTCTCTTCCC AGTCGTCAAG AAGGATATAA CATTTCTTCA TGAAGGAAAT
    GATTCAAAAG TTGATGGGTT AGTAAATTTT GAGAAGCTGC GAATGATTGC AAAGGAGATT
    CGACATGTTA TTCGCATGAC TTCTGCTAAT ATGGATCCAG CAGTTATGTT TAGGCAAAGG
    AAGAAGAGAT GGAGAAGTTT AGGGTCCCTA AGCCAAGGCA GCACAAACTC TAACATGCTG
    GATGTTCAAG GAGGTGGTCA CAAGAAACGG GCTCGGCGAA GTTCATTCCT TAATGCTAAG
    AAGCTGTATG AAGATGCACA GATGTCAAGG AAGGTAAAAC AGTACATCTC CAACCTGAGT
    GTGGAAATCG ATGAGGAGAA GATCCAGCTA ATGTCACTGC AATGTGAACC AGCCTACAGC
    ACTTTGGCAA AGAATTTAAG TGAGAGAAGA TCTGCAAAAT CTGACTTGTC TCCTGCACCT
    TTGAGGCCAT CAGGCCAAAC TACCAAAGCT CACATCCATC AGCAAAACAG AATGAGCCAA
    GTATTGCAAG TCCCTACAGT TAACTTATAT CCAATGCGAA AGAAAGCACC AACCAAGGAA
    CATCTTCCAT GCACTTCAGG ATCTTCACAG AAAATATCTG GTTTGACAGA TGAGGTTACA
    GTTAAAGCAC AGTTAGAAGA CTCTGTTTCT CTTGTGTCAT CATTGCATTC CAGCCCAGCC
    GCATCCCCTC AAGGATCTCC ACATAAAGTT GTTAACATTT CAAGATCAGA GAATTCCAGT
    CATATGAACC TCTCTGGATC CTCCTCATCA TTAACCAGTG ATCCCAACCA GAAGATCTGC
    AGTCGGCGTG GCTATGGAAT AGGCTATGCC CATAACCCCT CGGCCAAATC TGATAACTTC
    TCAGACTCAA GCAACAGTGA GATCTCGTCC CGATCAAGCC TCGTCAGCAA CTGCTCAGTA
    GACTCCATGT CGTTGACCCT GCAGGATGAA AAGTGTGTGC CTCATTCACA TGCTGAGTCA
    ATGGGACTAT CAGAAAGAAA AGACGTCCAT CCGTCCTCTG AGTATAACCA GCCCTGCAGT
    AGCTGGCTCC ATGCAAGGCA CCCTGCAGTC CGGAATTTCA TAGCTTGCGC AAGCAGTGAG
    GACATAGCTC ATGACCACAT ATCGGTAGAG CCGGTAGACA GTGGTCGGGG CAGTTGGACA
    TCTTGTTCTA GCAGTTCACA TGACAATTTC CAGACTTTGC AGAAGGGACT GGACCGCTTA
    AGCACTTACC GCCAATCACA CATTGATTCC CCAATTGCTG AGACAGAGTC CGCAAACTGC
    AGCACAGATG AAACATGTAG TGAAGACTGT ACCAAAGGAA AAAGGCACTC AAACAACAGT
    TTAGAACTAA ATGAATCTCG GCAAAGTTGG GCTTCATCTA GCTCAATGTC AGATACTTAC
    GAGACGAACT ATGGCACAAT CAAGCGGAGA GTATTGGACA CAGAGGAAAA AACAGAGGCT
    TTGGGCCAAC AATTAGATGT CTTTTATAAA ACAGTTACTT CCACTACTGA AAAAGGACTG
    ATAGTGTACT GTGTCACCTC ATCCCAGAAG GACAGTAGGT ATAGGGAGCC ACCTCCCACT
    CCTCCAGGCT ACCAGGGGAT ATCATTAGCC GACATAAAGG AAGGACAACC CCACCAAGCG
    CACTTAAAAC CTCCAGATTA TAGTGTGGCG ATACAGAGGT CAAAGATGAT ACATAAAGAC
    CTCTCTAGGC TTTCATCAGC CTGTGTTGAA CCAGTCTCAT GTGTCTCACA GAAGATGATA
    ACCCATTGGC ATAACCGGCA TCAGCCTTAT CCTAATGTAC AGGCTGACAG AGATAGTAAA
    GAAGATGAAG ATGAACAAAT GTCAGCAGTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa43162
    Clone ID Related Accession (Same CDS sequence) XM_018092139.2 , XM_018092139.1
    Accession Version XM_018092139.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4617bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 6 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018092139.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTCTGCCA GCATTGAACC CAGGTTTAAA GAAGCCTTGA GGAAAAAACC CAAGGAAAGG 
    ACTCAGGAGG ATCTGAAAAC TGTTTATTCA TATATGCATA GAATGGATAT ACTCTCACAT
    CTCAGAGAAC ATCAATTAAG ATTGATGTCT GCAACAGCAC GATATGAAAG ATACAGTGCA
    AATCAGGTTC TATTCTGCTC AGAAAGTTTG GCTAGATGTT GGTACATTCT TCTTTCTGGT
    TCAGTTCTGG TAAAAAACTC CATGTTTTTG CCTCCATGCA GTTTTGGCAA GCAGTCAGGT
    GGCAAAAGGG GTTGTGATTG CATTGTACTG GAACCTTCTG AAATGATTGT GGTGGAAAAT
    ACAGGAGAAA ACAATGATGG CATTCTGCAT AGGGAAACTT CTATTAGACA ATCCCGGCGT
    CGGTATCGAA AAATCAACCA GCATGGAGAA CGCCAAACAA TTACAGATAA TGGGGATGCT
    GCTAGTTATG TCTTGCTCCC CACAGACCTC TCCAAGATAC ACCTCACCGA CAGTCCCCAC
    CCACATGTTA CACATGTGCC ATCCAGCCAA TCAGGATGCA GCATTGCAAG CGACTCTGGA
    AGCAGCAGCT TATCAGATAT CTACCAGGCA ACAGAAAGTG AGATGGGGGA TGTTGACTTG
    ACAGGCCTGC CAGAGGCAGC TGTTGATTCT GAAGATGAAG AGGAAGATGA AGATATTGAG
    AGAGCTTCAG ACACAATGCT GACCCGTGAC ATTGTGAGGG AATGTCTTGA GAAAGAACCC
    ACAGACAGAA CAGATGATGA CATAGAACAG CTACTGGAAT TCATGCATCA GCTTCCAGCC
    TTTGTCAATA TGACCATGTC TGTGAGACGA AAGCTTTGCT CGGTCATGAT TTTTGAAGTT
    GTGGAACAGG CTGGCGCAAT CATCATTGAA GATGGGCAAG AACTGGACTC TTGGTATGTC
    ATTCTTAATG GGATTGTAGA AATCAGCCAT TCAGATGGCA GGATGGAAAA CCTTTGCATG
    GGAAATAGCT TTGGCATAAA CCCATCTTTA GAAACACAGT ACATGGATGG AGTAGTAAAA
    ACCAAAGCTG ATGATTGCCA GTTTGTGTGC ATAGCACAAC AGGACTACTT TCGGATATTA
    AACCATGTGG AAAAAAATAC CCACAAAGTC AAGGAAAAGG GGGAGATTGT TATGGTTAAT
    GAACACCGAG AACTGGATCG AAGTGGCACT CGAAAGGGAC ATATAGTAAT TCAGGGGACT
    CCAGAAAGAC TCATCTTGCA CCTAATAGAA GAGCACTCTG TTGTTGACCC AACATACATA
    GAGGATTTTC TATTAACATA CAGGACATTC CTTTCAAGTC CATTAGAGGT TGGGGACAAG
    CTTTTGGAGT GGTTTAAAGA TGACAGTCGT CGTGACAAGG TAACACGAGT TGTCCTTCTT
    TGGGTTAACA ACCACTTTAA TGATTTTGAA GGAGACCCTG CCATGACAAG GTTTTTGGAA
    GAATTTGAAC GGAATCTTGA AAATGCGAAA ATGAAGGGTC ATCTGCGACT GCTAAACATA
    GCCTGTGCTG CAAAGGCTAA ACTGAGACAG GTTACATTGC TTAAGCCTTC CCGTGAATCG
    CCTTTTTATT TCAACGTCGT TGGGGGAAGC GACAAAGGTT TCGGCATGTT TATTGAAAGT
    GTTGAGCCTG GAAGCAAAGC AACAGAAGCT GGATTAAAAC GCGGAGATCA GATTATGGAA
    GTCAATGGCC AAAATTTTGA GACTATCGTT TACGCCAAAG CCCTGGAGAT CTTGAAAAAC
    AATACACATC TTTCCATCAC TGTCAAGACC AACATTTTTG TGTTTAAAGA GCTTCTTAAT
    CGAATCGGGC AGGAGAAAAA AAATGGAGTC CCACACATCC CCAAAATTGC AGAGAAGAAG
    AACAATAGAT ATTCCATCCC TGATCTCCCC AATGATGTAG AACAGATGTT TCCACGGGAT
    CGCGCAAGCA AGAAAATGAA AGCCAATACG GTGTCTGGAG GGCGGAATCG CATCCGGAAG
    ATTTTGGACA AAACACGTTT TAGTATATTA CCACCTAAGC CATTCAGTGA TGGCCATGTT
    ATCCAGTCCC AGGATGACAG TATTGTTGGC ACCAGGCAGT GCAGGCACAG TGTGGCTATA
    ATGCCCATTC CGGGCACGCT CTCTTCAAGC AGTCCAGACT TGCTTCAAGC TACAACAAAC
    ATATTGGATT TCTCCAATCC TACTGATATT CCAGACCAAG TTATACGAAT ATTTAAAGCA
    GATCAGCAGT GCTGCTATAT TATCATCGGC AAAGACACTA CTGCAAAGGA GGTGGTGATA
    CAAGCACTTC ATGAATTCAG CCTTACTGGG TCAACAGACA CATACTCTCT CTGTGAGGTC
    TCGGTCAGTC CAGAAGGTGT CATTAAACAA AGACGGCTTC CGGATCAGCT TTCTAAACTG
    GCAGACAGAA TCCAGCTGAA TGGAAGGTAT TATTTGAAGA ACAACATGGA GACCGAAACG
    TTGTGTTCAG ATGAAGATGC TCAGGAGCTT CTGCGTGAAA GCCTCATCTC TCTCCTTCAG
    CTTAGCACTA TCGAGGTGGC AACACAGCTT TCTATGCGGG ACTTTGAACT CTTCCGCAAT
    ATTGAGCCCA CAGAATACAT AGATGACCTT TTCAAGATAG ATTCCAAGAC AAGTGCTGCT
    CATCTGAAAC AGTTTGAGGA AGTGATCAAC CAAGAGACAT TTTGGGTGGC CACGGAAATT
    CTAAGAGAAC CAAACCATTT GAAGAGAATG AAAATTGTGA AGCATTTCAT AAAGACTGCC
    CTCCATTGCA GAGAATGCAG AAATTTTAAC TCCATGTTTG CTATAATAAG TGGGCTCAAT
    CTTGCACCTG TGGCTCGGCT TCGAGGAACT TGGGAAAAGT TGCCCAGCAA ATACGAGAAG
    CTGCTTCGAG ACCTGCAAGA TCTCTTCGAC CCCTCAAGAA ATATGGCGAA ATACCGTAAT
    ATTCTAAGCA GTCAGAGCAT GCAGCCCCCC ATCATTCCTC TCTTCCCAGT CGTCAAGAAG
    GATATAACAT TTCTTCATGA AGGAAATGAT TCAAAAGTTG ATGGGTTAGT AAATTTTGAG
    AAGCTGCGAA TGATTGCAAA GGAGATTCGA CATGTTATTC GCATGACTTC TGCTAATATG
    GATCCAGCAG TTATGTTTAG GCAAAGGAAG AAGAGATGGA GAAGTTTAGG GTCCCTAAGC
    CAAGGCAGCA CAAACTCTAA CATGCTGGAT GTTCAAGGAG GTGGTCACAA GAAACGGGCT
    CGGCGAAGTT CATTCCTTAA TGCTAAGAAG CTGTATGAAG ATGCACAGAT GTCAAGGAAG
    GTAAAACAGT ACATCTCCAA CCTGAGTGTG GAAATCGATG AGGAGAAGAT CCAGCTAATG
    TCACTGCAAT GTGAACCAGC CTACAGCACT TTGGCAAAGA ATTTAAGTGA GAGAAGATCT
    GCAAAATCTG ACTTGTCTCC TGCACCTTTG AGGCCATCAG GCCAAACTAC CAAAGCTCAC
    ATCCATCAGC AAAACAGAAT GAGCCAAGTA TTGCAAGTCC CTACAGTTAA CTTATATCCA
    ATGCGAAAGA AAGCACCAAC CAAGGAACAT CTTCCATGCA CTTCAGGATC TTCACAGAAA
    ATATCTGGTT TGACAGATGA GGTTACAGTT AAAGCACAGT TAGAAGACTC TGTTTCTCTT
    GTGTCATCAT TGCATTCCAG CCCAGCCGCA TCCCCTCAAG GATCTCCACA TAAAGTTGTT
    AACATTTCAA GATCAGAGAA TTCCAGTCAT ATGAACCTCT CTGGATCCTC CTCATCATTA
    ACCAGTGATC CCAACCAGAA GATCTGCAGT CGGCGTGGCT ATGGAATAGG CTATGCCCAT
    AACCCCTCGG CCAAATCTGA TAACTTCTCA GACTCAAGCA ACAGTGAGAT CTCGTCCCGA
    TCAAGCCTCG TCAGCAACTG CTCAGTAGAC TCCATGTCGT TGACCCTGCA GGATGAAAAG
    TGTGTGCCTC ATTCACATGC TGAGTCAATG GGACTATCAG AAAGAAAAGA CGTCCATCCG
    TCCTCTGAGT ATAACCAGCC CTGCAGTAGC TGGCTCCATG CAAGGCACCC TGCAGTCCGG
    AATTTCATAG CTTGCGCAAG CAGTGAGGAC ATAGCTCATG ACCACATATC GGTAGAGCCG
    GTAGACAGTG GTCGGGGCAG TTGGACATCT TGTTCTAGCA GTTCACATGA CAATTTCCAG
    ACTTTGCAGA AGGGACTGGA CCGCTTAAGC ACTTACCGCC AATCACACAT TGATTCCCCA
    ATTGCTGAGA CAGAGTCCGC AAACTGCAGC ACAGATGAAA CATGTAGTGA AGACTGTACC
    AAAGGAAAAA GGCACTCAAA CAACAGTTTA GAACTAAATG AATCTCGGCA AAGTTGGGCT
    TCATCTAGCT CAATGTCAGA TACTTACGAG ACGAACTATG GCACAATCAA GCGGAGAGTA
    TTGGACACAG AGGAAAAAAC AGAGGCTTTG GGCCAACAAT TAGATGTCTT TTATAAAACA
    GTTACTTCCA CTACTGAAAA AGGACTGATA GAAGATGAAC AAATGTCAGC AGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947628.1
    CDS486..5102
    Translation

    Target ORF information:

    RefSeq Version XM_018092139.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 6 (rapgef6), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092139.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTCTGCCA GCATTGAACC CAGGTTTAAA GAAGCCTTGA GGAAAAAACC CAAGGAAAGG 
    ACTCAGGAGG ATCTGAAAAC TGTTTATTCA TATATGCATA GAATGGATAT ACTCTCACAT
    CTCAGAGAAC ATCAATTAAG ATTGATGTCT GCAACAGCAC GATATGAAAG ATACAGTGCA
    AATCAGGTTC TATTCTGCTC AGAAAGTTTG GCTAGATGTT GGTACATTCT TCTTTCTGGT
    TCAGTTCTGG TAAAAAACTC CATGTTTTTG CCTCCATGCA GTTTTGGCAA GCAGTCAGGT
    GGCAAAAGGG GTTGTGATTG CATTGTACTG GAACCTTCTG AAATGATTGT GGTGGAAAAT
    ACAGGAGAAA ACAATGATGG CATTCTGCAT AGGGAAACTT CTATTAGACA ATCCCGGCGT
    CGGTATCGAA AAATCAACCA GCATGGAGAA CGCCAAACAA TTACAGATAA TGGGGATGCT
    GCTAGTTATG TCTTGCTCCC CACAGACCTC TCCAAGATAC ACCTCACCGA CAGTCCCCAC
    CCACATGTTA CACATGTGCC ATCCAGCCAA TCAGGATGCA GCATTGCAAG CGACTCTGGA
    AGCAGCAGCT TATCAGATAT CTACCAGGCA ACAGAAAGTG AGATGGGGGA TGTTGACTTG
    ACAGGCCTGC CAGAGGCAGC TGTTGATTCT GAAGATGAAG AGGAAGATGA AGATATTGAG
    AGAGCTTCAG ACACAATGCT GACCCGTGAC ATTGTGAGGG AATGTCTTGA GAAAGAACCC
    ACAGACAGAA CAGATGATGA CATAGAACAG CTACTGGAAT TCATGCATCA GCTTCCAGCC
    TTTGTCAATA TGACCATGTC TGTGAGACGA AAGCTTTGCT CGGTCATGAT TTTTGAAGTT
    GTGGAACAGG CTGGCGCAAT CATCATTGAA GATGGGCAAG AACTGGACTC TTGGTATGTC
    ATTCTTAATG GGATTGTAGA AATCAGCCAT TCAGATGGCA GGATGGAAAA CCTTTGCATG
    GGAAATAGCT TTGGCATAAA CCCATCTTTA GAAACACAGT ACATGGATGG AGTAGTAAAA
    ACCAAAGCTG ATGATTGCCA GTTTGTGTGC ATAGCACAAC AGGACTACTT TCGGATATTA
    AACCATGTGG AAAAAAATAC CCACAAAGTC AAGGAAAAGG GGGAGATTGT TATGGTTAAT
    GAACACCGAG AACTGGATCG AAGTGGCACT CGAAAGGGAC ATATAGTAAT TCAGGGGACT
    CCAGAAAGAC TCATCTTGCA CCTAATAGAA GAGCACTCTG TTGTTGACCC AACATACATA
    GAGGATTTTC TATTAACATA CAGGACATTC CTTTCAAGTC CATTAGAGGT TGGGGACAAG
    CTTTTGGAGT GGTTTAAAGA TGACAGTCGT CGTGACAAGG TAACACGAGT TGTCCTTCTT
    TGGGTTAACA ACCACTTTAA TGATTTTGAA GGAGACCCTG CCATGACAAG GTTTTTGGAA
    GAATTTGAAC GGAATCTTGA AAATGCGAAA ATGAAGGGTC ATCTGCGACT GCTAAACATA
    GCCTGTGCTG CAAAGGCTAA ACTGAGACAG GTTACATTGC TTAAGCCTTC CCGTGAATCG
    CCTTTTTATT TCAACGTCGT TGGGGGAAGC GACAAAGGTT TCGGCATGTT TATTGAAAGT
    GTTGAGCCTG GAAGCAAAGC AACAGAAGCT GGATTAAAAC GCGGAGATCA GATTATGGAA
    GTCAATGGCC AAAATTTTGA GACTATCGTT TACGCCAAAG CCCTGGAGAT CTTGAAAAAC
    AATACACATC TTTCCATCAC TGTCAAGACC AACATTTTTG TGTTTAAAGA GCTTCTTAAT
    CGAATCGGGC AGGAGAAAAA AAATGGAGTC CCACACATCC CCAAAATTGC AGAGAAGAAG
    AACAATAGAT ATTCCATCCC TGATCTCCCC AATGATGTAG AACAGATGTT TCCACGGGAT
    CGCGCAAGCA AGAAAATGAA AGCCAATACG GTGTCTGGAG GGCGGAATCG CATCCGGAAG
    ATTTTGGACA AAACACGTTT TAGTATATTA CCACCTAAGC CATTCAGTGA TGGCCATGTT
    ATCCAGTCCC AGGATGACAG TATTGTTGGC ACCAGGCAGT GCAGGCACAG TGTGGCTATA
    ATGCCCATTC CGGGCACGCT CTCTTCAAGC AGTCCAGACT TGCTTCAAGC TACAACAAAC
    ATATTGGATT TCTCCAATCC TACTGATATT CCAGACCAAG TTATACGAAT ATTTAAAGCA
    GATCAGCAGT GCTGCTATAT TATCATCGGC AAAGACACTA CTGCAAAGGA GGTGGTGATA
    CAAGCACTTC ATGAATTCAG CCTTACTGGG TCAACAGACA CATACTCTCT CTGTGAGGTC
    TCGGTCAGTC CAGAAGGTGT CATTAAACAA AGACGGCTTC CGGATCAGCT TTCTAAACTG
    GCAGACAGAA TCCAGCTGAA TGGAAGGTAT TATTTGAAGA ACAACATGGA GACCGAAACG
    TTGTGTTCAG ATGAAGATGC TCAGGAGCTT CTGCGTGAAA GCCTCATCTC TCTCCTTCAG
    CTTAGCACTA TCGAGGTGGC AACACAGCTT TCTATGCGGG ACTTTGAACT CTTCCGCAAT
    ATTGAGCCCA CAGAATACAT AGATGACCTT TTCAAGATAG ATTCCAAGAC AAGTGCTGCT
    CATCTGAAAC AGTTTGAGGA AGTGATCAAC CAAGAGACAT TTTGGGTGGC CACGGAAATT
    CTAAGAGAAC CAAACCATTT GAAGAGAATG AAAATTGTGA AGCATTTCAT AAAGACTGCC
    CTCCATTGCA GAGAATGCAG AAATTTTAAC TCCATGTTTG CTATAATAAG TGGGCTCAAT
    CTTGCACCTG TGGCTCGGCT TCGAGGAACT TGGGAAAAGT TGCCCAGCAA ATACGAGAAG
    CTGCTTCGAG ACCTGCAAGA TCTCTTCGAC CCCTCAAGAA ATATGGCGAA ATACCGTAAT
    ATTCTAAGCA GTCAGAGCAT GCAGCCCCCC ATCATTCCTC TCTTCCCAGT CGTCAAGAAG
    GATATAACAT TTCTTCATGA AGGAAATGAT TCAAAAGTTG ATGGGTTAGT AAATTTTGAG
    AAGCTGCGAA TGATTGCAAA GGAGATTCGA CATGTTATTC GCATGACTTC TGCTAATATG
    GATCCAGCAG TTATGTTTAG GCAAAGGAAG AAGAGATGGA GAAGTTTAGG GTCCCTAAGC
    CAAGGCAGCA CAAACTCTAA CATGCTGGAT GTTCAAGGAG GTGGTCACAA GAAACGGGCT
    CGGCGAAGTT CATTCCTTAA TGCTAAGAAG CTGTATGAAG ATGCACAGAT GTCAAGGAAG
    GTAAAACAGT ACATCTCCAA CCTGAGTGTG GAAATCGATG AGGAGAAGAT CCAGCTAATG
    TCACTGCAAT GTGAACCAGC CTACAGCACT TTGGCAAAGA ATTTAAGTGA GAGAAGATCT
    GCAAAATCTG ACTTGTCTCC TGCACCTTTG AGGCCATCAG GCCAAACTAC CAAAGCTCAC
    ATCCATCAGC AAAACAGAAT GAGCCAAGTA TTGCAAGTCC CTACAGTTAA CTTATATCCA
    ATGCGAAAGA AAGCACCAAC CAAGGAACAT CTTCCATGCA CTTCAGGATC TTCACAGAAA
    ATATCTGGTT TGACAGATGA GGTTACAGTT AAAGCACAGT TAGAAGACTC TGTTTCTCTT
    GTGTCATCAT TGCATTCCAG CCCAGCCGCA TCCCCTCAAG GATCTCCACA TAAAGTTGTT
    AACATTTCAA GATCAGAGAA TTCCAGTCAT ATGAACCTCT CTGGATCCTC CTCATCATTA
    ACCAGTGATC CCAACCAGAA GATCTGCAGT CGGCGTGGCT ATGGAATAGG CTATGCCCAT
    AACCCCTCGG CCAAATCTGA TAACTTCTCA GACTCAAGCA ACAGTGAGAT CTCGTCCCGA
    TCAAGCCTCG TCAGCAACTG CTCAGTAGAC TCCATGTCGT TGACCCTGCA GGATGAAAAG
    TGTGTGCCTC ATTCACATGC TGAGTCAATG GGACTATCAG AAAGAAAAGA CGTCCATCCG
    TCCTCTGAGT ATAACCAGCC CTGCAGTAGC TGGCTCCATG CAAGGCACCC TGCAGTCCGG
    AATTTCATAG CTTGCGCAAG CAGTGAGGAC ATAGCTCATG ACCACATATC GGTAGAGCCG
    GTAGACAGTG GTCGGGGCAG TTGGACATCT TGTTCTAGCA GTTCACATGA CAATTTCCAG
    ACTTTGCAGA AGGGACTGGA CCGCTTAAGC ACTTACCGCC AATCACACAT TGATTCCCCA
    ATTGCTGAGA CAGAGTCCGC AAACTGCAGC ACAGATGAAA CATGTAGTGA AGACTGTACC
    AAAGGAAAAA GGCACTCAAA CAACAGTTTA GAACTAAATG AATCTCGGCA AAGTTGGGCT
    TCATCTAGCT CAATGTCAGA TACTTACGAG ACGAACTATG GCACAATCAA GCGGAGAGTA
    TTGGACACAG AGGAAAAAAC AGAGGCTTTG GGCCAACAAT TAGATGTCTT TTATAAAACA
    GTTACTTCCA CTACTGAAAA AGGACTGATA GAAGATGAAC AAATGTCAGC AGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa43162
    Clone ID Related Accession (Same CDS sequence) XM_018092139.2 , XM_018092139.1
    Accession Version XM_018092139.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4617bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rap guanine nucleotide exchange factor 6 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTCTGCCA GCATTGAACC CAGGTTTAAA GAAGCCTTGA GGAAAAAACC CAAGGAAAGG 
    ACTCAGGAGG ATCTGAAAAC TGTTTATTCA TATATGCATA GAATGGATAT ACTCTCACAT
    CTCAGAGAAC ATCAATTAAG ATTGATGTCT GCAACAGCAC GATATGAAAG ATACAGTGCA
    AATCAGGTTC TATTCTGCTC AGAAAGTTTG GCTAGATGTT GGTACATTCT TCTTTCTGGT
    TCAGTTCTGG TAAAAAACTC CATGTTTTTG CCTCCATGCA GTTTTGGCAA GCAGTCAGGT
    GGCAAAAGGG GTTGTGATTG CATTGTACTG GAACCTTCTG AAATGATTGT GGTGGAAAAT
    ACAGGAGAAA ACAATGATGG CATTCTGCAT AGGGAAACTT CTATTAGACA ATCCCGGCGT
    CGGTATCGAA AAATCAACCA GCATGGAGAA CGCCAAACAA TTACAGATAA TGGGGATGCT
    GCTAGTTATG TCTTGCTCCC CACAGACCTC TCCAAGATAC ACCTCACCGA CAGTCCCCAC
    CCACATGTTA CACATGTGCC ATCCAGCCAA TCAGGATGCA GCATTGCAAG CGACTCTGGA
    AGCAGCAGCT TATCAGATAT CTACCAGGCA ACAGAAAGTG AGATGGGGGA TGTTGACTTG
    ACAGGCCTGC CAGAGGCAGC TGTTGATTCT GAAGATGAAG AGGAAGATGA AGATATTGAG
    AGAGCTTCAG ACACAATGCT GACCCGTGAC ATTGTGAGGG AATGTCTTGA GAAAGAACCC
    ACAGACAGAA CAGATGATGA CATAGAACAG CTACTGGAAT TCATGCATCA GCTTCCAGCC
    TTTGTCAATA TGACCATGTC TGTGAGACGA AAGCTTTGCT CGGTCATGAT TTTTGAAGTT
    GTGGAACAGG CTGGCGCAAT CATCATTGAA GATGGGCAAG AACTGGACTC TTGGTATGTC
    ATTCTTAATG GGATTGTAGA AATCAGCCAT TCAGATGGCA GGATGGAAAA CCTTTGCATG
    GGAAATAGCT TTGGCATAAA CCCATCTTTA GAAACACAGT ACATGGATGG AGTAGTAAAA
    ACCAAAGCTG ATGATTGCCA GTTTGTGTGC ATAGCACAAC AGGACTACTT TCGGATATTA
    AACCATGTGG AAAAAAATAC CCACAAAGTC AAGGAAAAGG GGGAGATTGT TATGGTTAAT
    GAACACCGAG AACTGGATCG AAGTGGCACT CGAAAGGGAC ATATAGTAAT TCAGGGGACT
    CCAGAAAGAC TCATCTTGCA CCTAATAGAA GAGCACTCTG TTGTTGACCC AACATACATA
    GAGGATTTTC TATTAACATA CAGGACATTC CTTTCAAGTC CATTAGAGGT TGGGGACAAG
    CTTTTGGAGT GGTTTAAAGA TGACAGTCGT CGTGACAAGG TAACACGAGT TGTCCTTCTT
    TGGGTTAACA ACCACTTTAA TGATTTTGAA GGAGACCCTG CCATGACAAG GTTTTTGGAA
    GAATTTGAAC GGAATCTTGA AAATGCGAAA ATGAAGGGTC ATCTGCGACT GCTAAACATA
    GCCTGTGCTG CAAAGGCTAA ACTGAGACAG GTTACATTGC TTAAGCCTTC CCGTGAATCG
    CCTTTTTATT TCAACGTCGT TGGGGGAAGC GACAAAGGTT TCGGCATGTT TATTGAAAGT
    GTTGAGCCTG GAAGCAAAGC AACAGAAGCT GGATTAAAAC GCGGAGATCA GATTATGGAA
    GTCAATGGCC AAAATTTTGA GACTATCGTT TACGCCAAAG CCCTGGAGAT CTTGAAAAAC
    AATACACATC TTTCCATCAC TGTCAAGACC AACATTTTTG TGTTTAAAGA GCTTCTTAAT
    CGAATCGGGC AGGAGAAAAA AAATGGAGTC CCACACATCC CCAAAATTGC AGAGAAGAAG
    AACAATAGAT ATTCCATCCC TGATCTCCCC AATGATGTAG AACAGATGTT TCCACGGGAT
    CGCGCAAGCA AGAAAATGAA AGCCAATACG GTGTCTGGAG GGCGGAATCG CATCCGGAAG
    ATTTTGGACA AAACACGTTT TAGTATATTA CCACCTAAGC CATTCAGTGA TGGCCATGTT
    ATCCAGTCCC AGGATGACAG TATTGTTGGC ACCAGGCAGT GCAGGCACAG TGTGGCTATA
    ATGCCCATTC CGGGCACGCT CTCTTCAAGC AGTCCAGACT TGCTTCAAGC TACAACAAAC
    ATATTGGATT TCTCCAATCC TACTGATATT CCAGACCAAG TTATACGAAT ATTTAAAGCA
    GATCAGCAGT GCTGCTATAT TATCATCGGC AAAGACACTA CTGCAAAGGA GGTGGTGATA
    CAAGCACTTC ATGAATTCAG CCTTACTGGG TCAACAGACA CATACTCTCT CTGTGAGGTC
    TCGGTCAGTC CAGAAGGTGT CATTAAACAA AGACGGCTTC CGGATCAGCT TTCTAAACTG
    GCAGACAGAA TCCAGCTGAA TGGAAGGTAT TATTTGAAGA ACAACATGGA GACCGAAACG
    TTGTGTTCAG ATGAAGATGC TCAGGAGCTT CTGCGTGAAA GCCTCATCTC TCTCCTTCAG
    CTTAGCACTA TCGAGGTGGC AACACAGCTT TCTATGCGGG ACTTTGAACT CTTCCGCAAT
    ATTGAGCCCA CAGAATACAT AGATGACCTT TTCAAGATAG ATTCCAAGAC AAGTGCTGCT
    CATCTGAAAC AGTTTGAGGA AGTGATCAAC CAAGAGACAT TTTGGGTGGC CACGGAAATT
    CTAAGAGAAC CAAACCATTT GAAGAGAATG AAAATTGTGA AGCATTTCAT AAAGACTGCC
    CTCCATTGCA GAGAATGCAG AAATTTTAAC TCCATGTTTG CTATAATAAG TGGGCTCAAT
    CTTGCACCTG TGGCTCGGCT TCGAGGAACT TGGGAAAAGT TGCCCAGCAA ATACGAGAAG
    CTGCTTCGAG ACCTGCAAGA TCTCTTCGAC CCCTCAAGAA ATATGGCGAA ATACCGTAAT
    ATTCTAAGCA GTCAGAGCAT GCAGCCCCCC ATCATTCCTC TCTTCCCAGT CGTCAAGAAG
    GATATAACAT TTCTTCATGA AGGAAATGAT TCAAAAGTTG ATGGGTTAGT AAATTTTGAG
    AAGCTGCGAA TGATTGCAAA GGAGATTCGA CATGTTATTC GCATGACTTC TGCTAATATG
    GATCCAGCAG TTATGTTTAG GCAAAGGAAG AAGAGATGGA GAAGTTTAGG GTCCCTAAGC
    CAAGGCAGCA CAAACTCTAA CATGCTGGAT GTTCAAGGAG GTGGTCACAA GAAACGGGCT
    CGGCGAAGTT CATTCCTTAA TGCTAAGAAG CTGTATGAAG ATGCACAGAT GTCAAGGAAG
    GTAAAACAGT ACATCTCCAA CCTGAGTGTG GAAATCGATG AGGAGAAGAT CCAGCTAATG
    TCACTGCAAT GTGAACCAGC CTACAGCACT TTGGCAAAGA ATTTAAGTGA GAGAAGATCT
    GCAAAATCTG ACTTGTCTCC TGCACCTTTG AGGCCATCAG GCCAAACTAC CAAAGCTCAC
    ATCCATCAGC AAAACAGAAT GAGCCAAGTA TTGCAAGTCC CTACAGTTAA CTTATATCCA
    ATGCGAAAGA AAGCACCAAC CAAGGAACAT CTTCCATGCA CTTCAGGATC TTCACAGAAA
    ATATCTGGTT TGACAGATGA GGTTACAGTT AAAGCACAGT TAGAAGACTC TGTTTCTCTT
    GTGTCATCAT TGCATTCCAG CCCAGCCGCA TCCCCTCAAG GATCTCCACA TAAAGTTGTT
    AACATTTCAA GATCAGAGAA TTCCAGTCAT ATGAACCTCT CTGGATCCTC CTCATCATTA
    ACCAGTGATC CCAACCAGAA GATCTGCAGT CGGCGTGGCT ATGGAATAGG CTATGCCCAT
    AACCCCTCGG CCAAATCTGA TAACTTCTCA GACTCAAGCA ACAGTGAGAT CTCGTCCCGA
    TCAAGCCTCG TCAGCAACTG CTCAGTAGAC TCCATGTCGT TGACCCTGCA GGATGAAAAG
    TGTGTGCCTC ATTCACATGC TGAGTCAATG GGACTATCAG AAAGAAAAGA CGTCCATCCG
    TCCTCTGAGT ATAACCAGCC CTGCAGTAGC TGGCTCCATG CAAGGCACCC TGCAGTCCGG
    AATTTCATAG CTTGCGCAAG CAGTGAGGAC ATAGCTCATG ACCACATATC GGTAGAGCCG
    GTAGACAGTG GTCGGGGCAG TTGGACATCT TGTTCTAGCA GTTCACATGA CAATTTCCAG
    ACTTTGCAGA AGGGACTGGA CCGCTTAAGC ACTTACCGCC AATCACACAT TGATTCCCCA
    ATTGCTGAGA CAGAGTCCGC AAACTGCAGC ACAGATGAAA CATGTAGTGA AGACTGTACC
    AAAGGAAAAA GGCACTCAAA CAACAGTTTA GAACTAAATG AATCTCGGCA AAGTTGGGCT
    TCATCTAGCT CAATGTCAGA TACTTACGAG ACGAACTATG GCACAATCAA GCGGAGAGTA
    TTGGACACAG AGGAAAAAAC AGAGGCTTTG GGCCAACAAT TAGATGTCTT TTATAAAACA
    GTTACTTCCA CTACTGAAAA AGGACTGATA GAAGATGAAC AAATGTCAGC AGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947628.1
    CDS1359..5975
    Translation

    Target ORF information:

    RefSeq Version XM_018092139.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rap guanine nucleotide exchange factor 6 (rapgef6), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092139.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTCTGCCA GCATTGAACC CAGGTTTAAA GAAGCCTTGA GGAAAAAACC CAAGGAAAGG 
    ACTCAGGAGG ATCTGAAAAC TGTTTATTCA TATATGCATA GAATGGATAT ACTCTCACAT
    CTCAGAGAAC ATCAATTAAG ATTGATGTCT GCAACAGCAC GATATGAAAG ATACAGTGCA
    AATCAGGTTC TATTCTGCTC AGAAAGTTTG GCTAGATGTT GGTACATTCT TCTTTCTGGT
    TCAGTTCTGG TAAAAAACTC CATGTTTTTG CCTCCATGCA GTTTTGGCAA GCAGTCAGGT
    GGCAAAAGGG GTTGTGATTG CATTGTACTG GAACCTTCTG AAATGATTGT GGTGGAAAAT
    ACAGGAGAAA ACAATGATGG CATTCTGCAT AGGGAAACTT CTATTAGACA ATCCCGGCGT
    CGGTATCGAA AAATCAACCA GCATGGAGAA CGCCAAACAA TTACAGATAA TGGGGATGCT
    GCTAGTTATG TCTTGCTCCC CACAGACCTC TCCAAGATAC ACCTCACCGA CAGTCCCCAC
    CCACATGTTA CACATGTGCC ATCCAGCCAA TCAGGATGCA GCATTGCAAG CGACTCTGGA
    AGCAGCAGCT TATCAGATAT CTACCAGGCA ACAGAAAGTG AGATGGGGGA TGTTGACTTG
    ACAGGCCTGC CAGAGGCAGC TGTTGATTCT GAAGATGAAG AGGAAGATGA AGATATTGAG
    AGAGCTTCAG ACACAATGCT GACCCGTGAC ATTGTGAGGG AATGTCTTGA GAAAGAACCC
    ACAGACAGAA CAGATGATGA CATAGAACAG CTACTGGAAT TCATGCATCA GCTTCCAGCC
    TTTGTCAATA TGACCATGTC TGTGAGACGA AAGCTTTGCT CGGTCATGAT TTTTGAAGTT
    GTGGAACAGG CTGGCGCAAT CATCATTGAA GATGGGCAAG AACTGGACTC TTGGTATGTC
    ATTCTTAATG GGATTGTAGA AATCAGCCAT TCAGATGGCA GGATGGAAAA CCTTTGCATG
    GGAAATAGCT TTGGCATAAA CCCATCTTTA GAAACACAGT ACATGGATGG AGTAGTAAAA
    ACCAAAGCTG ATGATTGCCA GTTTGTGTGC ATAGCACAAC AGGACTACTT TCGGATATTA
    AACCATGTGG AAAAAAATAC CCACAAAGTC AAGGAAAAGG GGGAGATTGT TATGGTTAAT
    GAACACCGAG AACTGGATCG AAGTGGCACT CGAAAGGGAC ATATAGTAAT TCAGGGGACT
    CCAGAAAGAC TCATCTTGCA CCTAATAGAA GAGCACTCTG TTGTTGACCC AACATACATA
    GAGGATTTTC TATTAACATA CAGGACATTC CTTTCAAGTC CATTAGAGGT TGGGGACAAG
    CTTTTGGAGT GGTTTAAAGA TGACAGTCGT CGTGACAAGG TAACACGAGT TGTCCTTCTT
    TGGGTTAACA ACCACTTTAA TGATTTTGAA GGAGACCCTG CCATGACAAG GTTTTTGGAA
    GAATTTGAAC GGAATCTTGA AAATGCGAAA ATGAAGGGTC ATCTGCGACT GCTAAACATA
    GCCTGTGCTG CAAAGGCTAA ACTGAGACAG GTTACATTGC TTAAGCCTTC CCGTGAATCG
    CCTTTTTATT TCAACGTCGT TGGGGGAAGC GACAAAGGTT TCGGCATGTT TATTGAAAGT
    GTTGAGCCTG GAAGCAAAGC AACAGAAGCT GGATTAAAAC GCGGAGATCA GATTATGGAA
    GTCAATGGCC AAAATTTTGA GACTATCGTT TACGCCAAAG CCCTGGAGAT CTTGAAAAAC
    AATACACATC TTTCCATCAC TGTCAAGACC AACATTTTTG TGTTTAAAGA GCTTCTTAAT
    CGAATCGGGC AGGAGAAAAA AAATGGAGTC CCACACATCC CCAAAATTGC AGAGAAGAAG
    AACAATAGAT ATTCCATCCC TGATCTCCCC AATGATGTAG AACAGATGTT TCCACGGGAT
    CGCGCAAGCA AGAAAATGAA AGCCAATACG GTGTCTGGAG GGCGGAATCG CATCCGGAAG
    ATTTTGGACA AAACACGTTT TAGTATATTA CCACCTAAGC CATTCAGTGA TGGCCATGTT
    ATCCAGTCCC AGGATGACAG TATTGTTGGC ACCAGGCAGT GCAGGCACAG TGTGGCTATA
    ATGCCCATTC CGGGCACGCT CTCTTCAAGC AGTCCAGACT TGCTTCAAGC TACAACAAAC
    ATATTGGATT TCTCCAATCC TACTGATATT CCAGACCAAG TTATACGAAT ATTTAAAGCA
    GATCAGCAGT GCTGCTATAT TATCATCGGC AAAGACACTA CTGCAAAGGA GGTGGTGATA
    CAAGCACTTC ATGAATTCAG CCTTACTGGG TCAACAGACA CATACTCTCT CTGTGAGGTC
    TCGGTCAGTC CAGAAGGTGT CATTAAACAA AGACGGCTTC CGGATCAGCT TTCTAAACTG
    GCAGACAGAA TCCAGCTGAA TGGAAGGTAT TATTTGAAGA ACAACATGGA GACCGAAACG
    TTGTGTTCAG ATGAAGATGC TCAGGAGCTT CTGCGTGAAA GCCTCATCTC TCTCCTTCAG
    CTTAGCACTA TCGAGGTGGC AACACAGCTT TCTATGCGGG ACTTTGAACT CTTCCGCAAT
    ATTGAGCCCA CAGAATACAT AGATGACCTT TTCAAGATAG ATTCCAAGAC AAGTGCTGCT
    CATCTGAAAC AGTTTGAGGA AGTGATCAAC CAAGAGACAT TTTGGGTGGC CACGGAAATT
    CTAAGAGAAC CAAACCATTT GAAGAGAATG AAAATTGTGA AGCATTTCAT AAAGACTGCC
    CTCCATTGCA GAGAATGCAG AAATTTTAAC TCCATGTTTG CTATAATAAG TGGGCTCAAT
    CTTGCACCTG TGGCTCGGCT TCGAGGAACT TGGGAAAAGT TGCCCAGCAA ATACGAGAAG
    CTGCTTCGAG ACCTGCAAGA TCTCTTCGAC CCCTCAAGAA ATATGGCGAA ATACCGTAAT
    ATTCTAAGCA GTCAGAGCAT GCAGCCCCCC ATCATTCCTC TCTTCCCAGT CGTCAAGAAG
    GATATAACAT TTCTTCATGA AGGAAATGAT TCAAAAGTTG ATGGGTTAGT AAATTTTGAG
    AAGCTGCGAA TGATTGCAAA GGAGATTCGA CATGTTATTC GCATGACTTC TGCTAATATG
    GATCCAGCAG TTATGTTTAG GCAAAGGAAG AAGAGATGGA GAAGTTTAGG GTCCCTAAGC
    CAAGGCAGCA CAAACTCTAA CATGCTGGAT GTTCAAGGAG GTGGTCACAA GAAACGGGCT
    CGGCGAAGTT CATTCCTTAA TGCTAAGAAG CTGTATGAAG ATGCACAGAT GTCAAGGAAG
    GTAAAACAGT ACATCTCCAA CCTGAGTGTG GAAATCGATG AGGAGAAGAT CCAGCTAATG
    TCACTGCAAT GTGAACCAGC CTACAGCACT TTGGCAAAGA ATTTAAGTGA GAGAAGATCT
    GCAAAATCTG ACTTGTCTCC TGCACCTTTG AGGCCATCAG GCCAAACTAC CAAAGCTCAC
    ATCCATCAGC AAAACAGAAT GAGCCAAGTA TTGCAAGTCC CTACAGTTAA CTTATATCCA
    ATGCGAAAGA AAGCACCAAC CAAGGAACAT CTTCCATGCA CTTCAGGATC TTCACAGAAA
    ATATCTGGTT TGACAGATGA GGTTACAGTT AAAGCACAGT TAGAAGACTC TGTTTCTCTT
    GTGTCATCAT TGCATTCCAG CCCAGCCGCA TCCCCTCAAG GATCTCCACA TAAAGTTGTT
    AACATTTCAA GATCAGAGAA TTCCAGTCAT ATGAACCTCT CTGGATCCTC CTCATCATTA
    ACCAGTGATC CCAACCAGAA GATCTGCAGT CGGCGTGGCT ATGGAATAGG CTATGCCCAT
    AACCCCTCGG CCAAATCTGA TAACTTCTCA GACTCAAGCA ACAGTGAGAT CTCGTCCCGA
    TCAAGCCTCG TCAGCAACTG CTCAGTAGAC TCCATGTCGT TGACCCTGCA GGATGAAAAG
    TGTGTGCCTC ATTCACATGC TGAGTCAATG GGACTATCAG AAAGAAAAGA CGTCCATCCG
    TCCTCTGAGT ATAACCAGCC CTGCAGTAGC TGGCTCCATG CAAGGCACCC TGCAGTCCGG
    AATTTCATAG CTTGCGCAAG CAGTGAGGAC ATAGCTCATG ACCACATATC GGTAGAGCCG
    GTAGACAGTG GTCGGGGCAG TTGGACATCT TGTTCTAGCA GTTCACATGA CAATTTCCAG
    ACTTTGCAGA AGGGACTGGA CCGCTTAAGC ACTTACCGCC AATCACACAT TGATTCCCCA
    ATTGCTGAGA CAGAGTCCGC AAACTGCAGC ACAGATGAAA CATGTAGTGA AGACTGTACC
    AAAGGAAAAA GGCACTCAAA CAACAGTTTA GAACTAAATG AATCTCGGCA AAGTTGGGCT
    TCATCTAGCT CAATGTCAGA TACTTACGAG ACGAACTATG GCACAATCAA GCGGAGAGTA
    TTGGACACAG AGGAAAAAAC AGAGGCTTTG GGCCAACAAT TAGATGTCTT TTATAAAACA
    GTTACTTCCA CTACTGAAAA AGGACTGATA GAAGATGAAC AAATGTCAGC AGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.