LOC100488564 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following LOC100488564 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100488564 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa42306 XM_018090898.1
Latest version!
Xenopus tropicalis dysferlin (dysf), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa49535 XM_031895262.1
Latest version!
Xenopus tropicalis dysferlin (LOC100488564), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa49536 XM_031895263.1
Latest version!
Xenopus tropicalis dysferlin (LOC100488564), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa49537 XM_031895264.1
Latest version!
Xenopus tropicalis dysferlin (LOC100488564), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa49538 XM_031895265.1
Latest version!
Xenopus tropicalis dysferlin (LOC100488564), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa49539 XM_031895266.1
Latest version!
Xenopus tropicalis dysferlin (LOC100488564), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa49540 XM_031895267.1
Latest version!
Xenopus tropicalis dysferlin (LOC100488564), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa49541 XM_031895268.1
Latest version!
Xenopus tropicalis dysferlin (LOC100488564), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa49542 XM_031895269.1
Latest version!
Xenopus tropicalis dysferlin (LOC100488564), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa42306
    Clone ID Related Accession (Same CDS sequence) XM_018090898.1
    Accession Version XM_018090898.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6282bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product dysferlin
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGAGGG TCTTTATCCT TCATGCTGAA AATGTGCAAA CCCCAGACTC AGACATCAGC 
    GACTCCTACT GCTCTGTGGT GTTCCAAGGG GTTAAGAAGA AAACGAAAAT CATAAAGAAC
    AATCTGAATC CCATATGGAA CGAGGGATTC GAGTGGGATT TAAAAGGATT GCCCTTGGAC
    AACAGTTCGG AGCTTGTCGC GGTAGTGAAA GACCATGAAA CCATGGGAAG GAACAGGTTT
    CTGGGTGAGA CCCGAGTCAG CCTACGGGAT TTACTTACGT CCCCCAACCT GACGGCCACG
    TATAATGCCC CACTCCTGGA CAGCAAGAAG CAGGGCACGG GGGCGATATT AACCTTGCAG
    ATATCTTACT TTCCTCCACC GGGGGCAGCC CCAGTTGTAC CCCCTCCTGC ACCCAAGGAG
    CCCACCCCAT CGCTCAATGA GTTGGACACC GTCACTGATA CGGCTGGTGA AGAGGACACG
    GAAGACCAGG CTGGAACTGG CGATGAAGCG GATATTGTCT CTCTGGCCCC TGGAGGAGCA
    GAGCAACCCA CTCTCCCCAA GAAAACTGCC CCTGTCCCTG TGCAGGGATT AAAGAGAAAG
    AAGAGCAACT CCAAAAAACA CCTCTCCAAC AAGCCTCAGG ACTTCCAGGT TCGTGTTCGA
    GTGATAGAAG GGAGGCAGAT CCCAGGGATT AACATCAAAC CTGTCGTCAA AGTCACAGTT
    GCTGGGCAGA CCAAGAGAAC CCGGATACGC AAAGGGAACA ACCCATATTT CGATGAGACA
    TTTTTCTTCA ATTTTTTTGA GTCCCCTTCT GAACTCTTTG ATGAACCCGT GTTCCTGACT
    GTTCTCGATT CCCGCTCCCT CCGAACAGAC TCAGTGATTG GTGAATTCCG GATGGACGTC
    GGAATGATTT ATGGAGAATC AAAACATGCC TTCCTCAGGA AGTGGCTTCT CCTCTCCGAC
    CCAGACGATT TCTCCGCGGG GGCCAAGGGG TACCTGAAGG TCAGCCTCTA TGTACTTGGG
    CCTGGAGATG AGAGCCCGGT AGAGAAAAAG GAGGTCTCGG AAGACAAGGA GGACATTGAG
    GGGAACTTGC TGCGACCGGC CGGGGTGTCC CTGCGAGGAG CCCACCTTAC TCTGAAGATT
    TACAGGGCGG AAGATCTGCC GCAGATGGAC GATGCTGTGC TGGACAATGT CAAACAGATC
    TTTGGCTTTG ACAGCAACAA GAAAAACTTG GTGGATCCCT TTGTGGAAGT CAGTTTTGCG
    GGGAAGATGC TCCTCAGTAA GATCATGGAG AAGAACGCCA ACCCGCAGTG GAGTCAGTCC
    ATCACTCTTC CCATCAGGTT CCCCTCTATG TGTGAAAAAA TGCGCATCCG CCTGTTGGAC
    TGGGACCGAC TCACCCACAA CGATATCATC GGCACCACCT ACCTGAGCAT GTCCAAAATC
    TCAGCTCCTG GGGGCGAGAT TGAAGATGAG ACACCGCGTA AGGCCTTGCC GGTTTCTAAC
    TTTGATGACA CTTTGGGATT TCTGCCAACC TTCGGGCCTT GCTATGTGAA CCTTTATGGC
    AGCCCTCGGG AGTTCACTGG GTTTCCAGAC CCTTATGAAG ATCTGAACTT GGGGAAGGGA
    GAAGGAGTGG CTTACCGTGG TCGGGTCCTT CTGGAGTTGG AGACAAAGCT GGTGGAGCAG
    GCTGAGCAGA AGGTGGAAGA CATTCCTTCA GATGATATTT CACGGGTTGA GAAATATCTC
    CGCAGACGGA AGTACTGCCT CTTTGCCGCT TTCTATTCAG CCACCATGTT GCAGGACGTC
    GATGACGCCA TTCAGTTTGA GGTCAGCATT GGAAATTATG GAAACAAGTT CGACAACACC
    TGCCTCCCCC TGGCCTCCAC CACGCAGTAC AGCCGGGCTG TGTTCGACGG TAAGAGGCAC
    CCATTGTGGG ACGGGAGTTT TCCCTCGGTG CTAATTGAAA ATCATGCAAA AGGAAAACAT
    TGCAGACTGG ATAAAGCAGC GAATCTGGAC TGGGTCCACC TTGCTGTGAA AGCTAAGAGC
    TCCCCCTACG ACTTGGACGC TTTGGTCGCG CGTTTGTTTG ATGACATCAT CACCGACTGC
    AGCCACGCCC TGCCAGATGT CAAGTCTATG CAAGGGACCA CCCACCTGGA CCAGTACTTG
    TACAGATTCC GCAAGACCAA CCTTGCCCAG ATTGTCCAGG CGGCTCTGAA ACTCAAGCAC
    GATGACTGTG TGGAGCAGGA GAATGTGATG GAGCAGGCAG AGGACTGGCT AATGCGACTA
    AAGGCTATGG CTGAGGAGCC GCAGAACAGC CTGCCAGACA TAGTGATTTG GATGTTACAA
    GGGGAGAAGC GTGTGGCTTA TTATAGAATG CCGGCCCGCG ATGTCCTTTT CTCCAGGAAT
    GGCTCCAAGT GTTGCGGCAA GAACTGCGGG AAGCTCCAGA CAATATTTCT CAAATTTCCT
    CAGGAAGGAA CCCAAGGAGC GAAGATGCCA GCTCAGGTTC GAGTCCAGCT GTGGTTTGGG
    CTCGCTGTGG ATCAGAAGGA ATTCAATCAT TATGCTCAGG GGAAATTGTC TGTTTTTGCT
    GAAGCTTATG AAAACCAAAC AAAGCTGGCT CTCGTGGGCA ACTGGGGAAC AACTGGCCTT
    ACGTACCCCA AATACTCAGA CGTGACCGGG AAAATCAAAC TTCCAAAGGA GAGTTTCAAG
    GCTTCCACTG GATGGAGCTG GGCCGGAGAC TGGTTTATCA GCCCGGAGAA AACGTTGCTG
    TATGATACGG ACGCGGGTCA CATGAGCTTC GTGGAAGAGG TTTTCGAGAA CCAGGCCAGG
    CTGCCCGGTG GGCAATGGAT CCATATGACG GAGGCCTACT CAGATGTGAA TGGGGAGAAG
    GTTTTGCCAA AAGATGAGAT CGAATGCCCC CCTGGCTGGA AATGGGAGGA TGATGAATGG
    TCCACGGACA CCAACAGGGC TGTTGATGAG CAAGGATGGG AGTACGGCAT TACTATCCCC
    CCTGACCGGA AGCCTAAATC CTGGGTTCCA GCAGAGAAGA TGTATCACAC CAACCGCAGG
    AGGCGCTGGG TCAGACTAAG ACGAAGGGAC CCCAAGCAGA TTGAGATCCT AAAAAAGCAC
    AAGCCGGACG AGTCGGAGAG CGAGGGATGG GAATACGCCT CTCTCTTTGG CTGGAAGTTT
    CACCTGAAGT ACCGTAAGAC TGACAGTTTC CGCAGGCGCA GGTGGAGGCG CCGTATGGAA
    CCCCTGGAAC GCACCGGGGC GGCAGCTGTT TTCGCTTTGG AAGGTTCCCT GGGTGGAATT
    ACAGATGATA AAAGCGATGA TGGAAAAAAC ACGTCCACAA TGAGCTTCGG GGTCAACCGG
    CCGACGATAT CCTGCATATT TGACTATGGG AACCGTTACC ATTTGCGCTG CTACATATAC
    CAAGCGAGGA ACCTGGTGCC CATGGACCAA GACAGCTTTT CTGATCCATA CGCCATAGTC
    TCTTTCCTCC ATCAAAGTCA AAAGACCTTG GTGATGAAGA GCACCTTGAA CCCAACATGG
    GACCAGACCC TTATTTTTCA TGAAATTGAA GTTTTTGGAG ACCCTGAAAC CATAAAAGAA
    TCCCCTCCCA ACATAGTGGT GGAAATCTAT GACCACGATA CTTACGGTGC AGATGAATAC
    ATGGGAAGGT GCATTTGCAA GCCAAGCTTT GAGCGCTCCC CCCGGTTATC TTGGCACCCG
    GTGATCATGG GCACGAAGAA CGTCGGAGAG CTGCTCGCTG CCTTCGAGCT CATTCCCAGG
    GAAAAGCCGG CTGTTCATCT TATCCCAGGA CTGGAGGGAG AAATCTCAGG AGTTCTGAGC
    GAGCTGCTTA TTCCTCGCTT CTTCACCGAG AAACTTCTTG ACTGGTCATC AGACGCCGAC
    CTTCCCTACC CACCTCCACA GAGGGAGGCC AATGTGTACA TGGTGCCCCA GGGAATCAAA
    CCCGTTCTGC AGCGCACCGC TATAGAGATC CTGGCCTGGG GGCTGAGGAA CCTAAAGAGC
    TACCAGCTGG CCAGCGTGAC CTCCCCGAGT CTGATAGTGG AGTGCGGCGA TGAACTGATC
    CAGTCGGGGG TCATTAAGAA CGTCAAGAAG AACCCCAACT TTGATGTGTG CCTGCTCCTC
    ATGGAAGTGA GGTTGCCCAG AGAAGATCTA TATTGTCCAC CCATCATTGT GAAGGTCATC
    GATAACCGTC AGTTTGGAAG AAAACCAGTC GTCGGCCAAT GCACCATCAA GTCTTTGGAA
    GAGTTCCATT GTGACCCCAA CTCAGAAGAT CTAGAGGTAG AAGATGGACT GTCAGACAAT
    GTGAGTGTTA CACCCAGGGA CGAGGTGCTT ATTGACATTG ATGATAAGGA ACCTCTTATC
    CCGACTCAGC TTGCCGACGG TATGACCAGC TCAGCCATTA TTAACTTATC ATCTTCTCGG
    CCCAGTCTCC ATGAAGAAGA GTTCATTGAT TGGTGGAGTA AGTTTTATGC TTCCATTGGG
    GAAAAAGAGA AATGTGGAAC ATACCTAGAG AAAGGCTTTG ATACCTTGCA GGTACTGGAC
    ACAGAGCTAG AGAACATAGA GGATTACGAA GAACTCTCAG ACTTTTGCCG AACCTTCAAA
    CTGTACAGAG GAAAAACACA AGATGAGACA GAAGATCCCT CTGTGGTTGG AGAATTTAAG
    GGATCCTTTA AAATCTATCC TCTTCCTGAT GACCCATCAG TCCCCAGGCC AGCACCCCAG
    TTCCGCCAAC TCCCTGCCAA AGGTCTTCAA GAGTGCCTCG TGCGTGTCTA TATTGTTCGT
    GCTTTTGGTT TGCAGCCGAA AGACCCCAAC GGCAAGTGTG ATCCCTACGT GAAGATTTCC
    ATTGGAAAGA AGTCATTAAA TGATCAGGAA CATTATATCC CTTGCACCTT GGAGCCTGTT
    TTTGGAAAAA TGTTTGAGCT GACCTGCACC CTCCCTCTGG AGAAAGACCT GAAGGTCACA
    CTCTATGATT ATGACATGAT TTCAAAAGAT GAGAAGATTG GAGAGACGGT GATAGACCTG
    GAGAACCGTT TCCTCTCGAA ATATGGAGCA AGATGTGGAT TGCCTCAATC CTACTGCATA
    TCTGGGCCAA ACCAGTGGCG GGACCAACGG AAACCATCGG AACTTCTCAG AATCTTTGCG
    CAGCAGCGGA ACTTCAAACC CCCTAACTTC AAAAACAACA GCATAATGTT CCGGAACCAT
    GAGTACCATG TCTCTGAGCT GGAAGACAGC AAGTTCCCTA ACCCGCACCA GGGCCCGCCG
    GAGGAACGGC TCGCTCTACA CGTACTGAGA AGGCAAGGCC TGGTGTGTGA GCATGTGGAG
    ACCAGGGCCC TGTACAGCCC TTTGCAGCCG GATATAGAAC AGGGAAAGCT GCAGATGTGG
    GTTGACTTGT TCCCCAAGTC CCTCGGTTCT CCCGGCCCTC CCTTTAATAT AACCCCACGG
    AAAGCCAAAC GGTATTACTT GCGCTGTATC ATCTGGAACA CCAAAGATGT GATTCTGGAT
    GATGTGAGCA TCACAGGAGA GAAGATGAGT GATATCTATG TCAAGAGCTG GCTGATTGGC
    CATGAAGAAA ATAAACAGAA GACAGATGTT CACTACAGGT CTATGGGAGG TGAAGGGAAT
    TTTAACTGGC GGCTTGTGTT CCCTTTCGAC TATCTTCCCG CGGAGCAGGT CTGCTCAGTG
    TCCAGAAAGG AGCATTTCTG GAGCCTCGAC AAGACTGAGA ATAAAATCCC TCCAAACCTC
    ATTATACAGA TATGGGACAA CGATAAGTTC TCCTTCGATG ACTATTTAGG TTCCGTGCAG
    CTGGACCTCA ACCGGATGCC ACGACCTGCC AAAACGGCGG AGAAGTGCAG GCTGGACCTG
    CTGGATGAGG TGGAGTCTGC AAATCACTGC TCCTTGTTTG AGCAGAAGAC AGTCAAGGGA
    TGGTGGCCGT GTGTAGGGCA GGAGGGGGAC GAGAAGATTC TGGCTGGAAA AGTGGAAATT
    ACTTTAGAGA TTGTAAATGA ACAGGAACAT GATGAGAGAC CGGCCGGGAT TGGTCGAGAT
    GAGCCAAATA TGAACCCAAG ACTTGAAGAC CCAAAGCGCC CAGAAACCTC GTTCCTCTGG
    TTCACTTCCC CGTACAAGAC CCTGAAGTAC ATCCTGTGGA GGAGATACAA GTGGCTCATC
    CTTGTCTTCT TCCTTCTATT CATCCTACTG CTCTTCCTCG GAATCTTCCT GTATTCCTTC
    CCGAACTACG CTGCTATGAA GCTGGTGAAG CCGTTCAGTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017946387.1
    CDS146..6427
    Translation

    Target ORF information:

    RefSeq Version XM_018090898.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis dysferlin (dysf), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018090898.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGAGGG TCTTTATCCT TCATGCTGAA AATGTGCAAA CCCCAGACTC AGACATCAGC 
    GACTCCTACT GCTCTGTGGT GTTCCAAGGG GTTAAGAAGA AAACGAAAAT CATAAAGAAC
    AATCTGAATC CCATATGGAA CGAGGGATTC GAGTGGGATT TAAAAGGATT GCCCTTGGAC
    AACAGTTCGG AGCTTGTCGC GGTAGTGAAA GACCATGAAA CCATGGGAAG GAACAGGTTT
    CTGGGTGAGA CCCGAGTCAG CCTACGGGAT TTACTTACGT CCCCCAACCT GACGGCCACG
    TATAATGCCC CACTCCTGGA CAGCAAGAAG CAGGGCACGG GGGCGATATT AACCTTGCAG
    ATATCTTACT TTCCTCCACC GGGGGCAGCC CCAGTTGTAC CCCCTCCTGC ACCCAAGGAG
    CCCACCCCAT CGCTCAATGA GTTGGACACC GTCACTGATA CGGCTGGTGA AGAGGACACG
    GAAGACCAGG CTGGAACTGG CGATGAAGCG GATATTGTCT CTCTGGCCCC TGGAGGAGCA
    GAGCAACCCA CTCTCCCCAA GAAAACTGCC CCTGTCCCTG TGCAGGGATT AAAGAGAAAG
    AAGAGCAACT CCAAAAAACA CCTCTCCAAC AAGCCTCAGG ACTTCCAGGT TCGTGTTCGA
    GTGATAGAAG GGAGGCAGAT CCCAGGGATT AACATCAAAC CTGTCGTCAA AGTCACAGTT
    GCTGGGCAGA CCAAGAGAAC CCGGATACGC AAAGGGAACA ACCCATATTT CGATGAGACA
    TTTTTCTTCA ATTTTTTTGA GTCCCCTTCT GAACTCTTTG ATGAACCCGT GTTCCTGACT
    GTTCTCGATT CCCGCTCCCT CCGAACAGAC TCAGTGATTG GTGAATTCCG GATGGACGTC
    GGAATGATTT ATGGAGAATC AAAACATGCC TTCCTCAGGA AGTGGCTTCT CCTCTCCGAC
    CCAGACGATT TCTCCGCGGG GGCCAAGGGG TACCTGAAGG TCAGCCTCTA TGTACTTGGG
    CCTGGAGATG AGAGCCCGGT AGAGAAAAAG GAGGTCTCGG AAGACAAGGA GGACATTGAG
    GGGAACTTGC TGCGACCGGC CGGGGTGTCC CTGCGAGGAG CCCACCTTAC TCTGAAGATT
    TACAGGGCGG AAGATCTGCC GCAGATGGAC GATGCTGTGC TGGACAATGT CAAACAGATC
    TTTGGCTTTG ACAGCAACAA GAAAAACTTG GTGGATCCCT TTGTGGAAGT CAGTTTTGCG
    GGGAAGATGC TCCTCAGTAA GATCATGGAG AAGAACGCCA ACCCGCAGTG GAGTCAGTCC
    ATCACTCTTC CCATCAGGTT CCCCTCTATG TGTGAAAAAA TGCGCATCCG CCTGTTGGAC
    TGGGACCGAC TCACCCACAA CGATATCATC GGCACCACCT ACCTGAGCAT GTCCAAAATC
    TCAGCTCCTG GGGGCGAGAT TGAAGATGAG ACACCGCGTA AGGCCTTGCC GGTTTCTAAC
    TTTGATGACA CTTTGGGATT TCTGCCAACC TTCGGGCCTT GCTATGTGAA CCTTTATGGC
    AGCCCTCGGG AGTTCACTGG GTTTCCAGAC CCTTATGAAG ATCTGAACTT GGGGAAGGGA
    GAAGGAGTGG CTTACCGTGG TCGGGTCCTT CTGGAGTTGG AGACAAAGCT GGTGGAGCAG
    GCTGAGCAGA AGGTGGAAGA CATTCCTTCA GATGATATTT CACGGGTTGA GAAATATCTC
    CGCAGACGGA AGTACTGCCT CTTTGCCGCT TTCTATTCAG CCACCATGTT GCAGGACGTC
    GATGACGCCA TTCAGTTTGA GGTCAGCATT GGAAATTATG GAAACAAGTT CGACAACACC
    TGCCTCCCCC TGGCCTCCAC CACGCAGTAC AGCCGGGCTG TGTTCGACGG TAAGAGGCAC
    CCATTGTGGG ACGGGAGTTT TCCCTCGGTG CTAATTGAAA ATCATGCAAA AGGAAAACAT
    TGCAGACTGG ATAAAGCAGC GAATCTGGAC TGGGTCCACC TTGCTGTGAA AGCTAAGAGC
    TCCCCCTACG ACTTGGACGC TTTGGTCGCG CGTTTGTTTG ATGACATCAT CACCGACTGC
    AGCCACGCCC TGCCAGATGT CAAGTCTATG CAAGGGACCA CCCACCTGGA CCAGTACTTG
    TACAGATTCC GCAAGACCAA CCTTGCCCAG ATTGTCCAGG CGGCTCTGAA ACTCAAGCAC
    GATGACTGTG TGGAGCAGGA GAATGTGATG GAGCAGGCAG AGGACTGGCT AATGCGACTA
    AAGGCTATGG CTGAGGAGCC GCAGAACAGC CTGCCAGACA TAGTGATTTG GATGTTACAA
    GGGGAGAAGC GTGTGGCTTA TTATAGAATG CCGGCCCGCG ATGTCCTTTT CTCCAGGAAT
    GGCTCCAAGT GTTGCGGCAA GAACTGCGGG AAGCTCCAGA CAATATTTCT CAAATTTCCT
    CAGGAAGGAA CCCAAGGAGC GAAGATGCCA GCTCAGGTTC GAGTCCAGCT GTGGTTTGGG
    CTCGCTGTGG ATCAGAAGGA ATTCAATCAT TATGCTCAGG GGAAATTGTC TGTTTTTGCT
    GAAGCTTATG AAAACCAAAC AAAGCTGGCT CTCGTGGGCA ACTGGGGAAC AACTGGCCTT
    ACGTACCCCA AATACTCAGA CGTGACCGGG AAAATCAAAC TTCCAAAGGA GAGTTTCAAG
    GCTTCCACTG GATGGAGCTG GGCCGGAGAC TGGTTTATCA GCCCGGAGAA AACGTTGCTG
    TATGATACGG ACGCGGGTCA CATGAGCTTC GTGGAAGAGG TTTTCGAGAA CCAGGCCAGG
    CTGCCCGGTG GGCAATGGAT CCATATGACG GAGGCCTACT CAGATGTGAA TGGGGAGAAG
    GTTTTGCCAA AAGATGAGAT CGAATGCCCC CCTGGCTGGA AATGGGAGGA TGATGAATGG
    TCCACGGACA CCAACAGGGC TGTTGATGAG CAAGGATGGG AGTACGGCAT TACTATCCCC
    CCTGACCGGA AGCCTAAATC CTGGGTTCCA GCAGAGAAGA TGTATCACAC CAACCGCAGG
    AGGCGCTGGG TCAGACTAAG ACGAAGGGAC CCCAAGCAGA TTGAGATCCT AAAAAAGCAC
    AAGCCGGACG AGTCGGAGAG CGAGGGATGG GAATACGCCT CTCTCTTTGG CTGGAAGTTT
    CACCTGAAGT ACCGTAAGAC TGACAGTTTC CGCAGGCGCA GGTGGAGGCG CCGTATGGAA
    CCCCTGGAAC GCACCGGGGC GGCAGCTGTT TTCGCTTTGG AAGGTTCCCT GGGTGGAATT
    ACAGATGATA AAAGCGATGA TGGAAAAAAC ACGTCCACAA TGAGCTTCGG GGTCAACCGG
    CCGACGATAT CCTGCATATT TGACTATGGG AACCGTTACC ATTTGCGCTG CTACATATAC
    CAAGCGAGGA ACCTGGTGCC CATGGACCAA GACAGCTTTT CTGATCCATA CGCCATAGTC
    TCTTTCCTCC ATCAAAGTCA AAAGACCTTG GTGATGAAGA GCACCTTGAA CCCAACATGG
    GACCAGACCC TTATTTTTCA TGAAATTGAA GTTTTTGGAG ACCCTGAAAC CATAAAAGAA
    TCCCCTCCCA ACATAGTGGT GGAAATCTAT GACCACGATA CTTACGGTGC AGATGAATAC
    ATGGGAAGGT GCATTTGCAA GCCAAGCTTT GAGCGCTCCC CCCGGTTATC TTGGCACCCG
    GTGATCATGG GCACGAAGAA CGTCGGAGAG CTGCTCGCTG CCTTCGAGCT CATTCCCAGG
    GAAAAGCCGG CTGTTCATCT TATCCCAGGA CTGGAGGGAG AAATCTCAGG AGTTCTGAGC
    GAGCTGCTTA TTCCTCGCTT CTTCACCGAG AAACTTCTTG ACTGGTCATC AGACGCCGAC
    CTTCCCTACC CACCTCCACA GAGGGAGGCC AATGTGTACA TGGTGCCCCA GGGAATCAAA
    CCCGTTCTGC AGCGCACCGC TATAGAGATC CTGGCCTGGG GGCTGAGGAA CCTAAAGAGC
    TACCAGCTGG CCAGCGTGAC CTCCCCGAGT CTGATAGTGG AGTGCGGCGA TGAACTGATC
    CAGTCGGGGG TCATTAAGAA CGTCAAGAAG AACCCCAACT TTGATGTGTG CCTGCTCCTC
    ATGGAAGTGA GGTTGCCCAG AGAAGATCTA TATTGTCCAC CCATCATTGT GAAGGTCATC
    GATAACCGTC AGTTTGGAAG AAAACCAGTC GTCGGCCAAT GCACCATCAA GTCTTTGGAA
    GAGTTCCATT GTGACCCCAA CTCAGAAGAT CTAGAGGTAG AAGATGGACT GTCAGACAAT
    GTGAGTGTTA CACCCAGGGA CGAGGTGCTT ATTGACATTG ATGATAAGGA ACCTCTTATC
    CCGACTCAGC TTGCCGACGG TATGACCAGC TCAGCCATTA TTAACTTATC ATCTTCTCGG
    CCCAGTCTCC ATGAAGAAGA GTTCATTGAT TGGTGGAGTA AGTTTTATGC TTCCATTGGG
    GAAAAAGAGA AATGTGGAAC ATACCTAGAG AAAGGCTTTG ATACCTTGCA GGTACTGGAC
    ACAGAGCTAG AGAACATAGA GGATTACGAA GAACTCTCAG ACTTTTGCCG AACCTTCAAA
    CTGTACAGAG GAAAAACACA AGATGAGACA GAAGATCCCT CTGTGGTTGG AGAATTTAAG
    GGATCCTTTA AAATCTATCC TCTTCCTGAT GACCCATCAG TCCCCAGGCC AGCACCCCAG
    TTCCGCCAAC TCCCTGCCAA AGGTCTTCAA GAGTGCCTCG TGCGTGTCTA TATTGTTCGT
    GCTTTTGGTT TGCAGCCGAA AGACCCCAAC GGCAAGTGTG ATCCCTACGT GAAGATTTCC
    ATTGGAAAGA AGTCATTAAA TGATCAGGAA CATTATATCC CTTGCACCTT GGAGCCTGTT
    TTTGGAAAAA TGTTTGAGCT GACCTGCACC CTCCCTCTGG AGAAAGACCT GAAGGTCACA
    CTCTATGATT ATGACATGAT TTCAAAAGAT GAGAAGATTG GAGAGACGGT GATAGACCTG
    GAGAACCGTT TCCTCTCGAA ATATGGAGCA AGATGTGGAT TGCCTCAATC CTACTGCATA
    TCTGGGCCAA ACCAGTGGCG GGACCAACGG AAACCATCGG AACTTCTCAG AATCTTTGCG
    CAGCAGCGGA ACTTCAAACC CCCTAACTTC AAAAACAACA GCATAATGTT CCGGAACCAT
    GAGTACCATG TCTCTGAGCT GGAAGACAGC AAGTTCCCTA ACCCGCACCA GGGCCCGCCG
    GAGGAACGGC TCGCTCTACA CGTACTGAGA AGGCAAGGCC TGGTGTGTGA GCATGTGGAG
    ACCAGGGCCC TGTACAGCCC TTTGCAGCCG GATATAGAAC AGGGAAAGCT GCAGATGTGG
    GTTGACTTGT TCCCCAAGTC CCTCGGTTCT CCCGGCCCTC CCTTTAATAT AACCCCACGG
    AAAGCCAAAC GGTATTACTT GCGCTGTATC ATCTGGAACA CCAAAGATGT GATTCTGGAT
    GATGTGAGCA TCACAGGAGA GAAGATGAGT GATATCTATG TCAAGAGCTG GCTGATTGGC
    CATGAAGAAA ATAAACAGAA GACAGATGTT CACTACAGGT CTATGGGAGG TGAAGGGAAT
    TTTAACTGGC GGCTTGTGTT CCCTTTCGAC TATCTTCCCG CGGAGCAGGT CTGCTCAGTG
    TCCAGAAAGG AGCATTTCTG GAGCCTCGAC AAGACTGAGA ATAAAATCCC TCCAAACCTC
    ATTATACAGA TATGGGACAA CGATAAGTTC TCCTTCGATG ACTATTTAGG TTCCGTGCAG
    CTGGACCTCA ACCGGATGCC ACGACCTGCC AAAACGGCGG AGAAGTGCAG GCTGGACCTG
    CTGGATGAGG TGGAGTCTGC AAATCACTGC TCCTTGTTTG AGCAGAAGAC AGTCAAGGGA
    TGGTGGCCGT GTGTAGGGCA GGAGGGGGAC GAGAAGATTC TGGCTGGAAA AGTGGAAATT
    ACTTTAGAGA TTGTAAATGA ACAGGAACAT GATGAGAGAC CGGCCGGGAT TGGTCGAGAT
    GAGCCAAATA TGAACCCAAG ACTTGAAGAC CCAAAGCGCC CAGAAACCTC GTTCCTCTGG
    TTCACTTCCC CGTACAAGAC CCTGAAGTAC ATCCTGTGGA GGAGATACAA GTGGCTCATC
    CTTGTCTTCT TCCTTCTATT CATCCTACTG CTCTTCCTCG GAATCTTCCT GTATTCCTTC
    CCGAACTACG CTGCTATGAA GCTGGTGAAG CCGTTCAGTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa49535
    Clone ID Related Accession (Same CDS sequence) XM_031895262.1
    Accession Version XM_031895262.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6327bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product dysferlin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGCGCT GTGTGGTCCA AAGGGCCAGC AACCTCCCAA ATGTGGAGAA AGGGGTCAGG 
    TACAGTGACC CAGTTACCAG TTTAACCTTC AGAGGGGTTA AGAAGAAAAC GAAAATCATA
    AAGAACAATC TGAATCCCAT ATGGAACGAG GGATTCGAGT GGGATTTAAA AGGATTGCCC
    TTGGACAACA GTTCGGAGCT TGTCGCGGTA GTGAAAGACC ATGAAACCAT GGGAAGGAAC
    AGGTTTCTGG GTGAGACCCG AGTCAGCCTA CGGGATTTAC TTACGTCCCC TAACCTGACG
    GCCACGTATA ATGCCCCACT CCTGGACAGC AAGAAGCAGG GCACGGGGGC GATATTAACC
    TTGCAGATAT CTTACTTTCC TCCACCGGGG GCAGCCCCAG TTGTACCCCC TCCTGCACCC
    AAGGAGCCCA CCCCATCGCT CAATGAGTTG GACACCATCA CTGATACGGC TGGTGAAGAG
    GACACGGAAG ACCAGGCTGG AACTGGCGAT GAAGCGGATA TTGTCTCTCT GGCCCCTGGA
    GGAGCAGAGC AACCCACTCT CCCCAAGAAA ACTGCCCCTG TCCCTGTGCA GGGATTAAAG
    AGAAAGAAGA GCAACTCCAA AAAACACCTC TCCAACAAGC CTCAGGACTT CCAGGTTCGT
    GTTCGAGTGA TAGAAGGGAG GCAGATCCCA GGGATTAACA TCAAACCTGT TGTCAAAGTC
    ACAGTTGCTG GGCAGACCAA GAGAACCCGG ATACGCAAAG GGAACAACCC ATATTTCGAT
    GAGACATTTT TCTTCAATTT TTTTGAGTCC CCTTCTGAAC TCTTTGATGA ACCCGTGTTC
    CTGACTGTTC TCGATTCCCG CTCCCTCCGA ACAGACTCAG TGATTGGTGA ATTCCGGATG
    GACGTCGGAA TGATTTATGG AGAATCAAAA CATGCCTTCC TCAGGAAGTG GCTTCTCCTC
    TCCGACCCAG ACGATTTCTC CGCGGGGGCC AAGGGGTACC TGAAGGTCAG CCTCTATGTA
    CTTGGGCCTG GAGATGAGAG CCCGGTAGAG AAAAAGGAGG TCTCGGAAGA CAAGGAGGAC
    ATTGAGGGGA ACTTGCTGCG ACCGGCCGGG GTGTCCCTGC GAGGAGCCCA CCTTACTCTG
    AAGATTTACA GGGCGGAAGA TCTGCCGCAG ATGGACGATG CTGTGCTGGA CAATGTCAAA
    CAGATCTTTG GCTTTGACAG CAACAAGAAA AACTTGGTGG ATCCCTTTGT GGAAGTCAGT
    TTTGCGGGGA AGATGCTCCT CAGTAAGATC ATGGAGAAGA ACGCCAACCC GCAGTGGAGT
    CAGTCCATCA CTCTTCCCAT CAGGTTCCCC TCTATGTGTG AAAAAATGCG CATCCGCCTG
    TTGGACTGGG ACCGCCTCAC CCACAACGAT ATCATCGGCA CCACCTACCT GAGCATGTCC
    AAAATCTCAG CTCCTGGCGG CGAGATTGAA GATGAGACAC CGCGTAAGGC CTTGCCGGTT
    TCTAACTTTG ATGACACTTT GGGATTTCTG CCAACCTTCG GGCCTTGCTA TGTGAACCTT
    TATGGCAGCC CTCGGGAGTT CACTGGGTTT CCAGACCCTT ATGAAGATCT GAACTTGGGG
    AAGGGAGAAG GAGTGGCTTA CCGTGGTCGG GTCCTTCTGG AGTTGGAGAC AAAGCTGGTG
    GAGCAGGCTG AGCAGAAGGT GGAAGACATT CCTTCAGATG ATATTTCACG GGTTGAGAAA
    TATCTCCGCA GACGGAAGTA CTGCCTCTTT GCCGCTTTCT ATTCAGCCAC CATGTTGCAG
    GACGTCGATG ACGCCATTCA GTTTGAGGTC AGCATTGGAA ATTATGGAAA CAAGTTCGAC
    AACACCTGCC TCCCCCTGGC CTCCACCACG CAGTACAGCC GGGCTGTGTT CGACGGTTGC
    CAGTACTATT ATCTCCCCTG GGGTAACGTA AAGCCCGTTG TTGTTCTCTC ATCTCACTGG
    GAAGACATCG GCCACAGAAC TGACACTCAG AATATTCTAC AATACGCCAT CGATAATCTG
    GCAGCGAATC TGGACTGGGT CCACCTTGCT GTGAAAGCTA AGAGCTCCCC CTACGACTTG
    GACGCTTTGG TCGCGCGTTT GTTTGATGAC ATCATCACCG ACTGCAGCCA CGCCCTGCCA
    GATGTCAAGT CTATGCAAGG GACCACCCAC CTGGACCAGT ACTTGTACAG ATTCCGCAAG
    ACCAACCTTG CCCAGATTGT CCAGGCGGCT CTGAAACTCA AGCACGATGA CTGTGTGGAG
    CAGGAGAATG TGATGGAGCA GGCAGAGGAC TGGCTAATGC GACTAAAGGC TATGGCTGAG
    GAGCCGCAGA ACAGCCTGCC AGACATAGTG ATTTGGATGT TACAAGGGGA GAAGCGTGTG
    GCTTATTATA GAATGCCGGC CCGCGATGTC CTTTTCTCCA GGAATGGCTC CAAGTGTTGC
    GGCAAGAACT GCGGGAAGCT CCAGACAATA TTTCTCAAAT TTCCTCAGGA AGGAACCCAA
    GGAGCGAAGA TGCCAGCTCA GGTTCGAGTC CAGCTGTGGT TTGGGCTCGC TGTGGATCAG
    AAGGAATTCA ATCATTATGC TCAGGGGAAA TTGTCTGTTT TTGCTGAAGC TTATGAAAAC
    CAAACAAAGC TGGCTCTCGT GGGCAACTGG GGAACAACTG GCCTTACGTA CCCCAAATAC
    TCAGACGTGA CCGGGAAAAT CAAACTTCCA AAGGAGAGTT TCAAGGCTTC CACTGGATGG
    AGCTGGGCCG GAGACTGGTT TATCAGCCCG GAGAAAACGT TGCTGTATGA TACGGACGCG
    GGTCACATGA GCTTCGTGGA AGAGGTTTTC GAGAACCAGG CCAGGCTGCC CGGTGGGCAA
    TGGATCCATA TGACGGAGGC CTACTCAGAT GTGAATGGGG AGAAGGTTTT GCCAAAAGAT
    GAGATCGAAT GCCCCCCTGG CTGGAAATGG GAGGATGATG AATGGTCCAC GGACACCAAC
    AGGGCTGTTG ATGAGCAAGG ATGGGAGTAC GGCATTACTA TCCCCCCTGA CCGGAAGCCT
    AAATCCTGGG TTCCAGCAGA GAAGATGTAT CACACCAACC GCAGGAGGCG CTGGGTCAGA
    CTAAGACGAA GGGACCCCAA GCAGATTGAG ATCCTAAAAA AGCACAAGCC GGACGAGTCG
    GAGAGCGAGG GATGGGAATA CGCCTCTCTC TTTGGCTGGA AGTTTCACCT GAAGTACCGT
    AAGACTGACA GTTTCCGCAG GCGCAGGTGG AGGCGCCGTA TGGAACCCCT GGAACGCACC
    GGGGCGGCAG CTGTTTTCGC TTTGGAAGGT TCCCTGGGTG GAATTACAGA TGATAAAAGC
    GATGATGGAA AAAACACGTC CACAATGAGC TTCGGGGTCA ACCGGCCGAC GATATCCTGC
    ATATTTGACT ATGGGAACCG TTACCATTTG CGCTGCTACA TATACCAAGC GAGGAACCTG
    GTGCCCATGG ACCAAGACAG CTTTTCTGAT CCATACGCCA TAGTCTCTTT CCTCCATCAA
    AGTCAAAAGA CCTTGGTGAT GAAGAGCACC TTGAACCCAA CATGGGACCA GACCCTTATT
    TTTCATGAAA TTGAAGTTTT TGGAGACCCT GAAACCATAA AAGAATCCCC TCCCAACATA
    GTGGTGGAAA TCTATGACCA CGATACTTAC GGTGCAGATG AATACATGGG AAGGTGCATT
    TGCAAGCCAA GCTTTGAGCG CTCCCCCCGG TTATCTTGGC ACCCGGTGAT CATGGGCACG
    AAGAACGTCG GAGAGCTGCT CGCTGCCTTC GAGCTCATTC CCAGGGAAAA GCCGGCTGTT
    CATCTTATCC CAGGACTGGA GGGAGAAATC TCAGGAGTTC TGAGCGAGCT GCTTATTCCT
    CGCTTCTTCA CCGAGAAACT TCTTGACTGG TCATCAGACG CCGACCTTCC CTACCCACCT
    CCACAGAGGG AGGCCAATGT GTACATGGTG CCCCAGGGAA TCAAACCCGT TCTGCAGCGC
    ACCGCTATAG AGATCCTGGC CTGGGGGCTG AGGAACCTAA AGAGCTACCA GCTGGCCAGC
    GTGACCTCCC CGAGTCTGAT AGTGGAGTGC GGCGATGAAC TGATCCAGTC GGGGGTCATT
    AAGAACGTCA AGAAGAACCC CAACTTTGAT GTGTGCCTGC TCCTCATGGA AGTGAGGTTG
    CCCAGAGAAG ATCTATATTG TCCACCCATC ATTGTGAAGG TCATCGATAA CCGTCAGTTT
    GGAAGAAAAC CAGTCGTCGG CCAATGCACC ATCAAGTCTT TGGAAGAGTT CCATTGTGAC
    CCCAACTCAG AAGATCTAGA GGTAGAAGAT GGACTGTCAG ACAATGTGAG TGTTACACCC
    AGGGACGAGG TGCTTATTGA CATTGATGAT AAGGAACCTC TTATCCCGAC TCAGCTTGCC
    GACGGTATGA CCAGCTCAGC CATTATTAAC TTATCATCTT CTCGGCCCAG TCTCCATGAA
    GAAGAGTTCA TTGATTGGTG GAGTAAGTTT TATGCTTCCA TTGGGGAAAA AGAGAAATGT
    GGAACATACC TAGAGAAAGG CTTTGATACC TTGCAGGTAC TGGACACAGA GCTAGAGAAC
    ATAGAGGATT ACGAAGAACT CTCAGACTTT TGCCGAACCT TCAAACTGTA CAGAGGAAAA
    ACACAAGATG AGACAGAAGA TCCCTCTGTG GTTGGAGAAT TTAAGGGATC CTTTAAAATC
    TATCCTCTTC CTGATGACCC ATCAGTCCCC AGGCCAGCAC CCCAGTTCCG CCAACTCCCT
    GCCAAAGGTC TTCAAGAGTG CCTCGTGCGT GTCTATATTG TTCGTGCTTT TGGTTTGCAG
    CCGAAAGACC CCAACGGCAA GTGTGATCCC TACGTGAAGA TTTCCATTGG AAAGAAGTCA
    TTAAATGATC AGGAACATTA TATCCCTTGC ACCTTGGAGC CTGTTTTTGG AAAAATGTTT
    GAGCTGACCT GCACCCTCCC TCTGGAGAAA GACCTGAAGG TCACACTCTA TGATTATGAC
    ATGATTTCAA AAGATGAGAA GATTGGAGAG ACGGTGATAG ACCTGGAGAA CCGTTTCCTC
    TCGAAATATG GAGCAAGATG TGGATTGCCT CAATCCTACT GCATATCTGG GCCAAACCAG
    TGGCGGGACC AACGGAAACC ATCGGAACTT CTCAGAATCT TTGCGCAGCA GCGGAACTTC
    AAACCCCCTA ACTTCAAAAA CAACAGCATA ATGTTCCGGA ACCATGAGTA CCATGTCTCT
    GAGCTGGAAG ACAGCAAGTT CCCTAACCCG CACCAGGGCC CGCCGGAGGA ACGGCTCGCT
    CTACACGTAC TGAGAAGGCA AGGCCTGGTG TGTGAGCATG TGGAGACCAG GGCCCTGTAC
    AGCCCTTTGC AGCCGGATAT AGAACAGGGA AAGCTGCAGA TGTGGGTTGA CTTGTTCCCC
    AAGTCCCTCG GTTCTCCCGG CCCTCCCTTT AATATAACCC CACGGAAAGC CAAACGGTAT
    TACTTGCGCT GTATCATCTG GAACACCAAA GATGTGATTC TGGATGATGT GAGCATCACA
    GGAGAGAAGA TGAGTGATAT CTATGTCAAG AGCTGGCTGA TTGGCCATGA AGAAAATAAA
    CAGAAGACAG ATGTTCACTA CAGGTCTATG GGAGGTGAAG GGAATTTTAA CTGGCGGCTT
    GTGTTCCCTT TCGACTATCT TCCCGCGGAG CAGGTCTGCT CAGTGTCCAG AAAGGAGCAT
    TTCTGGAGCC TCGACAAGAC TGAGAATAAA ATCCCTCCAA ACCTCATTAT ACAGATATGG
    GACAACGATA AGTTCTCCTT CGATGACTAT TTAGGTTCCG TGCAGCTGGA CCTCAACCGG
    ATGCCACGAC CTGCCAAAAC GGCGGAGAAG TGCAGGCTGG ACCTGCTGGA TGAGGTGGAG
    TCTGCAAATC ACTGCTCCTT GTTTGAGCAG AAGACAGTCA AGGGATGGTG GCCGTGTGTA
    GGGCAGGAGG GGGACGAGAA GATTCTGGCT GGAAAAGTGG AAATTACTTT AGAGATTGTA
    AATGAACAGG AACATGATGA GAGACCGGCC GGGATTGGTC GAGATGAGCC AAATATGAAC
    CCAAGACTTG AAGACCCAAA GCGCCCAGAA ACCTCGTTCC TCTGGTTCAC TTCCCCGTAC
    AAGACCCTGA AGTACATCCT GTGGAGGAGA TACAAGTGGC TCATCCTTGT CTTCTTCCTT
    CTATTCATCC TACTGCTCTT CCTCGGAATC TTCCTGTATT CCTTCCCGAA CTACGCTGCT
    ATGAAGCTGG TGAAGCCGTT CAGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751122.1
    CDS1273..7599
    Translation

    Target ORF information:

    RefSeq Version XM_031895262.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis dysferlin (LOC100488564), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031895262.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGCGCT GTGTGGTCCA AAGGGCCAGC AACCTCCCAA ATGTGGAGAA AGGGGTCAGG 
    TACAGTGACC CAGTTACCAG TTTAACCTTC AGAGGGGTTA AGAAGAAAAC GAAAATCATA
    AAGAACAATC TGAATCCCAT ATGGAACGAG GGATTCGAGT GGGATTTAAA AGGATTGCCC
    TTGGACAACA GTTCGGAGCT TGTCGCGGTA GTGAAAGACC ATGAAACCAT GGGAAGGAAC
    AGGTTTCTGG GTGAGACCCG AGTCAGCCTA CGGGATTTAC TTACGTCCCC TAACCTGACG
    GCCACGTATA ATGCCCCACT CCTGGACAGC AAGAAGCAGG GCACGGGGGC GATATTAACC
    TTGCAGATAT CTTACTTTCC TCCACCGGGG GCAGCCCCAG TTGTACCCCC TCCTGCACCC
    AAGGAGCCCA CCCCATCGCT CAATGAGTTG GACACCATCA CTGATACGGC TGGTGAAGAG
    GACACGGAAG ACCAGGCTGG AACTGGCGAT GAAGCGGATA TTGTCTCTCT GGCCCCTGGA
    GGAGCAGAGC AACCCACTCT CCCCAAGAAA ACTGCCCCTG TCCCTGTGCA GGGATTAAAG
    AGAAAGAAGA GCAACTCCAA AAAACACCTC TCCAACAAGC CTCAGGACTT CCAGGTTCGT
    GTTCGAGTGA TAGAAGGGAG GCAGATCCCA GGGATTAACA TCAAACCTGT TGTCAAAGTC
    ACAGTTGCTG GGCAGACCAA GAGAACCCGG ATACGCAAAG GGAACAACCC ATATTTCGAT
    GAGACATTTT TCTTCAATTT TTTTGAGTCC CCTTCTGAAC TCTTTGATGA ACCCGTGTTC
    CTGACTGTTC TCGATTCCCG CTCCCTCCGA ACAGACTCAG TGATTGGTGA ATTCCGGATG
    GACGTCGGAA TGATTTATGG AGAATCAAAA CATGCCTTCC TCAGGAAGTG GCTTCTCCTC
    TCCGACCCAG ACGATTTCTC CGCGGGGGCC AAGGGGTACC TGAAGGTCAG CCTCTATGTA
    CTTGGGCCTG GAGATGAGAG CCCGGTAGAG AAAAAGGAGG TCTCGGAAGA CAAGGAGGAC
    ATTGAGGGGA ACTTGCTGCG ACCGGCCGGG GTGTCCCTGC GAGGAGCCCA CCTTACTCTG
    AAGATTTACA GGGCGGAAGA TCTGCCGCAG ATGGACGATG CTGTGCTGGA CAATGTCAAA
    CAGATCTTTG GCTTTGACAG CAACAAGAAA AACTTGGTGG ATCCCTTTGT GGAAGTCAGT
    TTTGCGGGGA AGATGCTCCT CAGTAAGATC ATGGAGAAGA ACGCCAACCC GCAGTGGAGT
    CAGTCCATCA CTCTTCCCAT CAGGTTCCCC TCTATGTGTG AAAAAATGCG CATCCGCCTG
    TTGGACTGGG ACCGCCTCAC CCACAACGAT ATCATCGGCA CCACCTACCT GAGCATGTCC
    AAAATCTCAG CTCCTGGCGG CGAGATTGAA GATGAGACAC CGCGTAAGGC CTTGCCGGTT
    TCTAACTTTG ATGACACTTT GGGATTTCTG CCAACCTTCG GGCCTTGCTA TGTGAACCTT
    TATGGCAGCC CTCGGGAGTT CACTGGGTTT CCAGACCCTT ATGAAGATCT GAACTTGGGG
    AAGGGAGAAG GAGTGGCTTA CCGTGGTCGG GTCCTTCTGG AGTTGGAGAC AAAGCTGGTG
    GAGCAGGCTG AGCAGAAGGT GGAAGACATT CCTTCAGATG ATATTTCACG GGTTGAGAAA
    TATCTCCGCA GACGGAAGTA CTGCCTCTTT GCCGCTTTCT ATTCAGCCAC CATGTTGCAG
    GACGTCGATG ACGCCATTCA GTTTGAGGTC AGCATTGGAA ATTATGGAAA CAAGTTCGAC
    AACACCTGCC TCCCCCTGGC CTCCACCACG CAGTACAGCC GGGCTGTGTT CGACGGTTGC
    CAGTACTATT ATCTCCCCTG GGGTAACGTA AAGCCCGTTG TTGTTCTCTC ATCTCACTGG
    GAAGACATCG GCCACAGAAC TGACACTCAG AATATTCTAC AATACGCCAT CGATAATCTG
    GCAGCGAATC TGGACTGGGT CCACCTTGCT GTGAAAGCTA AGAGCTCCCC CTACGACTTG
    GACGCTTTGG TCGCGCGTTT GTTTGATGAC ATCATCACCG ACTGCAGCCA CGCCCTGCCA
    GATGTCAAGT CTATGCAAGG GACCACCCAC CTGGACCAGT ACTTGTACAG ATTCCGCAAG
    ACCAACCTTG CCCAGATTGT CCAGGCGGCT CTGAAACTCA AGCACGATGA CTGTGTGGAG
    CAGGAGAATG TGATGGAGCA GGCAGAGGAC TGGCTAATGC GACTAAAGGC TATGGCTGAG
    GAGCCGCAGA ACAGCCTGCC AGACATAGTG ATTTGGATGT TACAAGGGGA GAAGCGTGTG
    GCTTATTATA GAATGCCGGC CCGCGATGTC CTTTTCTCCA GGAATGGCTC CAAGTGTTGC
    GGCAAGAACT GCGGGAAGCT CCAGACAATA TTTCTCAAAT TTCCTCAGGA AGGAACCCAA
    GGAGCGAAGA TGCCAGCTCA GGTTCGAGTC CAGCTGTGGT TTGGGCTCGC TGTGGATCAG
    AAGGAATTCA ATCATTATGC TCAGGGGAAA TTGTCTGTTT TTGCTGAAGC TTATGAAAAC
    CAAACAAAGC TGGCTCTCGT GGGCAACTGG GGAACAACTG GCCTTACGTA CCCCAAATAC
    TCAGACGTGA CCGGGAAAAT CAAACTTCCA AAGGAGAGTT TCAAGGCTTC CACTGGATGG
    AGCTGGGCCG GAGACTGGTT TATCAGCCCG GAGAAAACGT TGCTGTATGA TACGGACGCG
    GGTCACATGA GCTTCGTGGA AGAGGTTTTC GAGAACCAGG CCAGGCTGCC CGGTGGGCAA
    TGGATCCATA TGACGGAGGC CTACTCAGAT GTGAATGGGG AGAAGGTTTT GCCAAAAGAT
    GAGATCGAAT GCCCCCCTGG CTGGAAATGG GAGGATGATG AATGGTCCAC GGACACCAAC
    AGGGCTGTTG ATGAGCAAGG ATGGGAGTAC GGCATTACTA TCCCCCCTGA CCGGAAGCCT
    AAATCCTGGG TTCCAGCAGA GAAGATGTAT CACACCAACC GCAGGAGGCG CTGGGTCAGA
    CTAAGACGAA GGGACCCCAA GCAGATTGAG ATCCTAAAAA AGCACAAGCC GGACGAGTCG
    GAGAGCGAGG GATGGGAATA CGCCTCTCTC TTTGGCTGGA AGTTTCACCT GAAGTACCGT
    AAGACTGACA GTTTCCGCAG GCGCAGGTGG AGGCGCCGTA TGGAACCCCT GGAACGCACC
    GGGGCGGCAG CTGTTTTCGC TTTGGAAGGT TCCCTGGGTG GAATTACAGA TGATAAAAGC
    GATGATGGAA AAAACACGTC CACAATGAGC TTCGGGGTCA ACCGGCCGAC GATATCCTGC
    ATATTTGACT ATGGGAACCG TTACCATTTG CGCTGCTACA TATACCAAGC GAGGAACCTG
    GTGCCCATGG ACCAAGACAG CTTTTCTGAT CCATACGCCA TAGTCTCTTT CCTCCATCAA
    AGTCAAAAGA CCTTGGTGAT GAAGAGCACC TTGAACCCAA CATGGGACCA GACCCTTATT
    TTTCATGAAA TTGAAGTTTT TGGAGACCCT GAAACCATAA AAGAATCCCC TCCCAACATA
    GTGGTGGAAA TCTATGACCA CGATACTTAC GGTGCAGATG AATACATGGG AAGGTGCATT
    TGCAAGCCAA GCTTTGAGCG CTCCCCCCGG TTATCTTGGC ACCCGGTGAT CATGGGCACG
    AAGAACGTCG GAGAGCTGCT CGCTGCCTTC GAGCTCATTC CCAGGGAAAA GCCGGCTGTT
    CATCTTATCC CAGGACTGGA GGGAGAAATC TCAGGAGTTC TGAGCGAGCT GCTTATTCCT
    CGCTTCTTCA CCGAGAAACT TCTTGACTGG TCATCAGACG CCGACCTTCC CTACCCACCT
    CCACAGAGGG AGGCCAATGT GTACATGGTG CCCCAGGGAA TCAAACCCGT TCTGCAGCGC
    ACCGCTATAG AGATCCTGGC CTGGGGGCTG AGGAACCTAA AGAGCTACCA GCTGGCCAGC
    GTGACCTCCC CGAGTCTGAT AGTGGAGTGC GGCGATGAAC TGATCCAGTC GGGGGTCATT
    AAGAACGTCA AGAAGAACCC CAACTTTGAT GTGTGCCTGC TCCTCATGGA AGTGAGGTTG
    CCCAGAGAAG ATCTATATTG TCCACCCATC ATTGTGAAGG TCATCGATAA CCGTCAGTTT
    GGAAGAAAAC CAGTCGTCGG CCAATGCACC ATCAAGTCTT TGGAAGAGTT CCATTGTGAC
    CCCAACTCAG AAGATCTAGA GGTAGAAGAT GGACTGTCAG ACAATGTGAG TGTTACACCC
    AGGGACGAGG TGCTTATTGA CATTGATGAT AAGGAACCTC TTATCCCGAC TCAGCTTGCC
    GACGGTATGA CCAGCTCAGC CATTATTAAC TTATCATCTT CTCGGCCCAG TCTCCATGAA
    GAAGAGTTCA TTGATTGGTG GAGTAAGTTT TATGCTTCCA TTGGGGAAAA AGAGAAATGT
    GGAACATACC TAGAGAAAGG CTTTGATACC TTGCAGGTAC TGGACACAGA GCTAGAGAAC
    ATAGAGGATT ACGAAGAACT CTCAGACTTT TGCCGAACCT TCAAACTGTA CAGAGGAAAA
    ACACAAGATG AGACAGAAGA TCCCTCTGTG GTTGGAGAAT TTAAGGGATC CTTTAAAATC
    TATCCTCTTC CTGATGACCC ATCAGTCCCC AGGCCAGCAC CCCAGTTCCG CCAACTCCCT
    GCCAAAGGTC TTCAAGAGTG CCTCGTGCGT GTCTATATTG TTCGTGCTTT TGGTTTGCAG
    CCGAAAGACC CCAACGGCAA GTGTGATCCC TACGTGAAGA TTTCCATTGG AAAGAAGTCA
    TTAAATGATC AGGAACATTA TATCCCTTGC ACCTTGGAGC CTGTTTTTGG AAAAATGTTT
    GAGCTGACCT GCACCCTCCC TCTGGAGAAA GACCTGAAGG TCACACTCTA TGATTATGAC
    ATGATTTCAA AAGATGAGAA GATTGGAGAG ACGGTGATAG ACCTGGAGAA CCGTTTCCTC
    TCGAAATATG GAGCAAGATG TGGATTGCCT CAATCCTACT GCATATCTGG GCCAAACCAG
    TGGCGGGACC AACGGAAACC ATCGGAACTT CTCAGAATCT TTGCGCAGCA GCGGAACTTC
    AAACCCCCTA ACTTCAAAAA CAACAGCATA ATGTTCCGGA ACCATGAGTA CCATGTCTCT
    GAGCTGGAAG ACAGCAAGTT CCCTAACCCG CACCAGGGCC CGCCGGAGGA ACGGCTCGCT
    CTACACGTAC TGAGAAGGCA AGGCCTGGTG TGTGAGCATG TGGAGACCAG GGCCCTGTAC
    AGCCCTTTGC AGCCGGATAT AGAACAGGGA AAGCTGCAGA TGTGGGTTGA CTTGTTCCCC
    AAGTCCCTCG GTTCTCCCGG CCCTCCCTTT AATATAACCC CACGGAAAGC CAAACGGTAT
    TACTTGCGCT GTATCATCTG GAACACCAAA GATGTGATTC TGGATGATGT GAGCATCACA
    GGAGAGAAGA TGAGTGATAT CTATGTCAAG AGCTGGCTGA TTGGCCATGA AGAAAATAAA
    CAGAAGACAG ATGTTCACTA CAGGTCTATG GGAGGTGAAG GGAATTTTAA CTGGCGGCTT
    GTGTTCCCTT TCGACTATCT TCCCGCGGAG CAGGTCTGCT CAGTGTCCAG AAAGGAGCAT
    TTCTGGAGCC TCGACAAGAC TGAGAATAAA ATCCCTCCAA ACCTCATTAT ACAGATATGG
    GACAACGATA AGTTCTCCTT CGATGACTAT TTAGGTTCCG TGCAGCTGGA CCTCAACCGG
    ATGCCACGAC CTGCCAAAAC GGCGGAGAAG TGCAGGCTGG ACCTGCTGGA TGAGGTGGAG
    TCTGCAAATC ACTGCTCCTT GTTTGAGCAG AAGACAGTCA AGGGATGGTG GCCGTGTGTA
    GGGCAGGAGG GGGACGAGAA GATTCTGGCT GGAAAAGTGG AAATTACTTT AGAGATTGTA
    AATGAACAGG AACATGATGA GAGACCGGCC GGGATTGGTC GAGATGAGCC AAATATGAAC
    CCAAGACTTG AAGACCCAAA GCGCCCAGAA ACCTCGTTCC TCTGGTTCAC TTCCCCGTAC
    AAGACCCTGA AGTACATCCT GTGGAGGAGA TACAAGTGGC TCATCCTTGT CTTCTTCCTT
    CTATTCATCC TACTGCTCTT CCTCGGAATC TTCCTGTATT CCTTCCCGAA CTACGCTGCT
    ATGAAGCTGG TGAAGCCGTT CAGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa49536
    Clone ID Related Accession (Same CDS sequence) XM_031895263.1
    Accession Version XM_031895263.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6321bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product dysferlin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGAGGG TCTTTATCCT TCATGCTGAA AATGTGCAAA CCCCAGACTC AGACATCAGC 
    GACTCCTACT GCTCTGTGGT GTTCCAAGGG GTTAAGAAGA AAACGAAAAT CATAAAGAAC
    AATCTGAATC CCATATGGAA CGAGGGATTC GAGTGGGATT TAAAAGGATT GCCCTTGGAC
    AACAGTTCGG AGCTTGTCGC GGTAGTGAAA GACCATGAAA CCATGGGAAG GAACAGGTTT
    CTGGGTGAGA CCCGAGTCAG CCTACGGGAT TTACTTACGT CCCCTAACCT GACGGCCACG
    TATAATGCCC CACTCCTGGA CAGCAAGAAG CAGGGCACGG GGGCGATATT AACCTTGCAG
    ATATCTTACT TTCCTCCACC GGGGGCAGCC CCAGTTGTAC CCCCTCCTGC ACCCAAGGAG
    CCCACCCCAT CGCTCAATGA GTTGGACACC ATCACTGATA CGGCTGGTGA AGAGGACACG
    GAAGACCAGG CTGGAACTGG CGATGAAGCG GATATTGTCT CTCTGGCCCC TGGAGGAGCA
    GAGCAACCCA CTCTCCCCAA GAAAACTGCC CCTGTCCCTG TGCAGGGATT AAAGAGAAAG
    AAGAGCAACT CCAAAAAACA CCTCTCCAAC AAGCCTCAGG ACTTCCAGGT TCGTGTTCGA
    GTGATAGAAG GGAGGCAGAT CCCAGGGATT AACATCAAAC CTGTTGTCAA AGTCACAGTT
    GCTGGGCAGA CCAAGAGAAC CCGGATACGC AAAGGGAACA ACCCATATTT CGATGAGACA
    TTTTTCTTCA ATTTTTTTGA GTCCCCTTCT GAACTCTTTG ATGAACCCGT GTTCCTGACT
    GTTCTCGATT CCCGCTCCCT CCGAACAGAC TCAGTGATTG GTGAATTCCG GATGGACGTC
    GGAATGATTT ATGGAGAATC AAAACATGCC TTCCTCAGGA AGTGGCTTCT CCTCTCCGAC
    CCAGACGATT TCTCCGCGGG GGCCAAGGGG TACCTGAAGG TCAGCCTCTA TGTACTTGGG
    CCTGGAGATG AGAGCCCGGT AGAGAAAAAG GAGGTCTCGG AAGACAAGGA GGACATTGAG
    GGGAACTTGC TGCGACCGGC CGGGGTGTCC CTGCGAGGAG CCCACCTTAC TCTGAAGATT
    TACAGGGCGG AAGATCTGCC GCAGATGGAC GATGCTGTGC TGGACAATGT CAAACAGATC
    TTTGGCTTTG ACAGCAACAA GAAAAACTTG GTGGATCCCT TTGTGGAAGT CAGTTTTGCG
    GGGAAGATGC TCCTCAGTAA GATCATGGAG AAGAACGCCA ACCCGCAGTG GAGTCAGTCC
    ATCACTCTTC CCATCAGGTT CCCCTCTATG TGTGAAAAAA TGCGCATCCG CCTGTTGGAC
    TGGGACCGCC TCACCCACAA CGATATCATC GGCACCACCT ACCTGAGCAT GTCCAAAATC
    TCAGCTCCTG GCGGCGAGAT TGAAGATGAG ACACCGCGTA AGGCCTTGCC GGTTTCTAAC
    TTTGATGACA CTTTGGGATT TCTGCCAACC TTCGGGCCTT GCTATGTGAA CCTTTATGGC
    AGCCCTCGGG AGTTCACTGG GTTTCCAGAC CCTTATGAAG ATCTGAACTT GGGGAAGGGA
    GAAGGAGTGG CTTACCGTGG TCGGGTCCTT CTGGAGTTGG AGACAAAGCT GGTGGAGCAG
    GCTGAGCAGA AGGTGGAAGA CATTCCTTCA GATGATATTT CACGGGTTGA GAAATATCTC
    CGCAGACGGA AGTACTGCCT CTTTGCCGCT TTCTATTCAG CCACCATGTT GCAGGACGTC
    GATGACGCCA TTCAGTTTGA GGTCAGCATT GGAAATTATG GAAACAAGTT CGACAACACC
    TGCCTCCCCC TGGCCTCCAC CACGCAGTAC AGCCGGGCTG TGTTCGACGG TTGCCAGTAC
    TATTATCTCC CCTGGGGTAA CGTAAAGCCC GTTGTTGTTC TCTCATCTCA CTGGGAAGAC
    ATCGGCCACA GAACTGACAC TCAGAATATT CTACAATACG CCATCGATAA TCTGGCAGCG
    AATCTGGACT GGGTCCACCT TGCTGTGAAA GCTAAGAGCT CCCCCTACGA CTTGGACGCT
    TTGGTCGCGC GTTTGTTTGA TGACATCATC ACCGACTGCA GCCACGCCCT GCCAGATGTC
    AAGTCTATGC AAGGGACCAC CCACCTGGAC CAGTACTTGT ACAGATTCCG CAAGACCAAC
    CTTGCCCAGA TTGTCCAGGC GGCTCTGAAA CTCAAGCACG ATGACTGTGT GGAGCAGGAG
    AATGTGATGG AGCAGGCAGA GGACTGGCTA ATGCGACTAA AGGCTATGGC TGAGGAGCCG
    CAGAACAGCC TGCCAGACAT AGTGATTTGG ATGTTACAAG GGGAGAAGCG TGTGGCTTAT
    TATAGAATGC CGGCCCGCGA TGTCCTTTTC TCCAGGAATG GCTCCAAGTG TTGCGGCAAG
    AACTGCGGGA AGCTCCAGAC AATATTTCTC AAATTTCCTC AGGAAGGAAC CCAAGGAGCG
    AAGATGCCAG CTCAGGTTCG AGTCCAGCTG TGGTTTGGGC TCGCTGTGGA TCAGAAGGAA
    TTCAATCATT ATGCTCAGGG GAAATTGTCT GTTTTTGCTG AAGCTTATGA AAACCAAACA
    AAGCTGGCTC TCGTGGGCAA CTGGGGAACA ACTGGCCTTA CGTACCCCAA ATACTCAGAC
    GTGACCGGGA AAATCAAACT TCCAAAGGAG AGTTTCAAGG CTTCCACTGG ATGGAGCTGG
    GCCGGAGACT GGTTTATCAG CCCGGAGAAA ACGTTGCTGT ATGATACGGA CGCGGGTCAC
    ATGAGCTTCG TGGAAGAGGT TTTCGAGAAC CAGGCCAGGC TGCCCGGTGG GCAATGGATC
    CATATGACGG AGGCCTACTC AGATGTGAAT GGGGAGAAGG TTTTGCCAAA AGATGAGATC
    GAATGCCCCC CTGGCTGGAA ATGGGAGGAT GATGAATGGT CCACGGACAC CAACAGGGCT
    GTTGATGAGC AAGGATGGGA GTACGGCATT ACTATCCCCC CTGACCGGAA GCCTAAATCC
    TGGGTTCCAG CAGAGAAGAT GTATCACACC AACCGCAGGA GGCGCTGGGT CAGACTAAGA
    CGAAGGGACC CCAAGCAGAT TGAGATCCTA AAAAAGCACA AGCCGGACGA GTCGGAGAGC
    GAGGGATGGG AATACGCCTC TCTCTTTGGC TGGAAGTTTC ACCTGAAGTA CCGTAAGACT
    GACAGTTTCC GCAGGCGCAG GTGGAGGCGC CGTATGGAAC CCCTGGAACG CACCGGGGCG
    GCAGCTGTTT TCGCTTTGGA AGGTTCCCTG GGTGGAATTA CAGATGATAA AAGCGATGAT
    GGAAAAAACA CGTCCACAAT GAGCTTCGGG GTCAACCGGC CGACGATATC CTGCATATTT
    GACTATGGGA ACCGTTACCA TTTGCGCTGC TACATATACC AAGCGAGGAA CCTGGTGCCC
    ATGGACCAAG ACAGCTTTTC TGATCCATAC GCCATAGTCT CTTTCCTCCA TCAAAGTCAA
    AAGACCTTGG TGATGAAGAG CACCTTGAAC CCAACATGGG ACCAGACCCT TATTTTTCAT
    GAAATTGAAG TTTTTGGAGA CCCTGAAACC ATAAAAGAAT CCCCTCCCAA CATAGTGGTG
    GAAATCTATG ACCACGATAC TTACGGTGCA GATGAATACA TGGGAAGGTG CATTTGCAAG
    CCAAGCTTTG AGCGCTCCCC CCGGTTATCT TGGCACCCGG TGATCATGGG CACGAAGAAC
    GTCGGAGAGC TGCTCGCTGC CTTCGAGCTC ATTCCCAGGG AAAAGCCGGC TGTTCATCTT
    ATCCCAGGAC TGGAGGGAGA AATCTCAGGA GTTCTGAGCG AGCTGCTTAT TCCTCGCTTC
    TTCACCGAGA AACTTCTTGA CTGGTCATCA GACGCCGACC TTCCCTACCC ACCTCCACAG
    AGGGAGGCCA ATGTGTACAT GGTGCCCCAG GGAATCAAAC CCGTTCTGCA GCGCACCGCT
    ATAGAGATCC TGGCCTGGGG GCTGAGGAAC CTAAAGAGCT ACCAGCTGGC CAGCGTGACC
    TCCCCGAGTC TGATAGTGGA GTGCGGCGAT GAACTGATCC AGTCGGGGGT CATTAAGAAC
    GTCAAGAAGA ACCCCAACTT TGATGTGTGC CTGCTCCTCA TGGAAGTGAG GTTGCCCAGA
    GAAGATCTAT ATTGTCCACC CATCATTGTG AAGGTCATCG ATAACCGTCA GTTTGGAAGA
    AAACCAGTCG TCGGCCAATG CACCATCAAG TCTTTGGAAG AGTTCCATTG TGACCCCAAC
    TCAGAAGATC TAGAGGTAGA AGATGGACTG TCAGACAATG TGAGTGTTAC ACCCAGGGAC
    GAGGTGCTTA TTGACATTGA TGATAAGGAA CCTCTTATCC CGACTCAGCT TGCCGACGGT
    ATGACCAGCT CAGCCATTAT TAACTTATCA TCTTCTCGGC CCAGTCTCCA TGAAGAAGAG
    TTCATTGATT GGTGGAGTAA GTTTTATGCT TCCATTGGGG AAAAAGAGAA ATGTGGAACA
    TACCTAGAGA AAGGCTTTGA TACCTTGCAG GTACTGGACA CAGAGCTAGA GAACATAGAG
    GATTACGAAG AACTCTCAGA CTTTTGCCGA ACCTTCAAAC TGTACAGAGG AAAAACACAA
    GATGAGACAG AAGATCCCTC TGTGGTTGGA GAATTTAAGG GATCCTTTAA AATCTATCCT
    CTTCCTGATG ACCCATCAGT CCCCAGGCCA GCACCCCAGT TCCGCCAACT CCCTGCCAAA
    GGTCTTCAAG AGTGCCTCGT GCGTGTCTAT ATTGTTCGTG CTTTTGGTTT GCAGCCGAAA
    GACCCCAACG GCAAGTGTGA TCCCTACGTG AAGATTTCCA TTGGAAAGAA GTCATTAAAT
    GATCAGGAAC ATTATATCCC TTGCACCTTG GAGCCTGTTT TTGGAAAAAT GTTTGAGCTG
    ACCTGCACCC TCCCTCTGGA GAAAGACCTG AAGGTCACAC TCTATGATTA TGACATGATT
    TCAAAAGATG AGAAGATTGG AGAGACGGTG ATAGACCTGG AGAACCGTTT CCTCTCGAAA
    TATGGAGCAA GATGTGGATT GCCTCAATCC TACTGCATAT CTGGGCCAAA CCAGTGGCGG
    GACCAACGGA AACCATCGGA ACTTCTCAGA ATCTTTGCGC AGCAGCGGAA CTTCAAACCC
    CCTAACTTCA AAAACAACAG CATAATGTTC CGGAACCATG AGTACCATGT CTCTGAGCTG
    GAAGACAGCA AGTTCCCTAA CCCGCACCAG GGCCCGCCGG AGGAACGGCT CGCTCTACAC
    GTACTGAGAA GGCAAGGCCT GGTGTGTGAG CATGTGGAGA CCAGGGCCCT GTACAGCCCT
    TTGCAGCCGG ATATAGAACA GGGAAAGCTG CAGATGTGGG TTGACTTGTT CCCCAAGTCC
    CTCGGTTCTC CCGGCCCTCC CTTTAATATA ACCCCACGGA AAGCCAAACG GTATTACTTG
    CGCTGTATCA TCTGGAACAC CAAAGATGTG ATTCTGGATG ATGTGAGCAT CACAGGAGAG
    AAGATGAGTG ATATCTATGT CAAGAGCTGG CTGATTGGCC ATGAAGAAAA TAAACAGAAG
    ACAGATGTTC ACTACAGGTC TATGGGAGGT GAAGGGAATT TTAACTGGCG GCTTGTGTTC
    CCTTTCGACT ATCTTCCCGC GGAGCAGGTC TGCTCAGTGT CCAGAAAGGA GCATTTCTGG
    AGCCTCGACA AGACTGAGAA TAAAATCCCT CCAAACCTCA TTATACAGAT ATGGGACAAC
    GATAAGTTCT CCTTCGATGA CTATTTAGGT TCCGTGCAGC TGGACCTCAA CCGGATGCCA
    CGACCTGCCA AAACGGCGGA GAAGTGCAGG CTGGACCTGC TGGATGAGGT GGAGTCTGCA
    AATCACTGCT CCTTGTTTGA GCAGAAGACA GTCAAGGGAT GGTGGCCGTG TGTAGGGCAG
    GAGGGGGACG AGAAGATTCT GGCTGGAAAA GTGGAAATTA CTTTAGAGAT TGTAAATGAA
    CAGGAACATG ATGAGAGACC GGCCGGGATT GGTCGAGATG AGCCAAATAT GAACCCAAGA
    CTTGAAGACC CAAAGCGCCC AGAAACCTCG TTCCTCTGGT TCACTTCCCC GTACAAGACC
    CTGAAGTACA TCCTGTGGAG GAGATACAAG TGGCTCATCC TTGTCTTCTT CCTTCTATTC
    ATCCTACTGC TCTTCCTCGG AATCTTCCTG TATTCCTTCC CGAACTACGC TGCTATGAAG
    CTGGTGAAGC CGTTCAGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751123.1
    CDS139..6459
    Translation

    Target ORF information:

    RefSeq Version XM_031895263.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis dysferlin (LOC100488564), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031895263.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGAGGG TCTTTATCCT TCATGCTGAA AATGTGCAAA CCCCAGACTC AGACATCAGC 
    GACTCCTACT GCTCTGTGGT GTTCCAAGGG GTTAAGAAGA AAACGAAAAT CATAAAGAAC
    AATCTGAATC CCATATGGAA CGAGGGATTC GAGTGGGATT TAAAAGGATT GCCCTTGGAC
    AACAGTTCGG AGCTTGTCGC GGTAGTGAAA GACCATGAAA CCATGGGAAG GAACAGGTTT
    CTGGGTGAGA CCCGAGTCAG CCTACGGGAT TTACTTACGT CCCCTAACCT GACGGCCACG
    TATAATGCCC CACTCCTGGA CAGCAAGAAG CAGGGCACGG GGGCGATATT AACCTTGCAG
    ATATCTTACT TTCCTCCACC GGGGGCAGCC CCAGTTGTAC CCCCTCCTGC ACCCAAGGAG
    CCCACCCCAT CGCTCAATGA GTTGGACACC ATCACTGATA CGGCTGGTGA AGAGGACACG
    GAAGACCAGG CTGGAACTGG CGATGAAGCG GATATTGTCT CTCTGGCCCC TGGAGGAGCA
    GAGCAACCCA CTCTCCCCAA GAAAACTGCC CCTGTCCCTG TGCAGGGATT AAAGAGAAAG
    AAGAGCAACT CCAAAAAACA CCTCTCCAAC AAGCCTCAGG ACTTCCAGGT TCGTGTTCGA
    GTGATAGAAG GGAGGCAGAT CCCAGGGATT AACATCAAAC CTGTTGTCAA AGTCACAGTT
    GCTGGGCAGA CCAAGAGAAC CCGGATACGC AAAGGGAACA ACCCATATTT CGATGAGACA
    TTTTTCTTCA ATTTTTTTGA GTCCCCTTCT GAACTCTTTG ATGAACCCGT GTTCCTGACT
    GTTCTCGATT CCCGCTCCCT CCGAACAGAC TCAGTGATTG GTGAATTCCG GATGGACGTC
    GGAATGATTT ATGGAGAATC AAAACATGCC TTCCTCAGGA AGTGGCTTCT CCTCTCCGAC
    CCAGACGATT TCTCCGCGGG GGCCAAGGGG TACCTGAAGG TCAGCCTCTA TGTACTTGGG
    CCTGGAGATG AGAGCCCGGT AGAGAAAAAG GAGGTCTCGG AAGACAAGGA GGACATTGAG
    GGGAACTTGC TGCGACCGGC CGGGGTGTCC CTGCGAGGAG CCCACCTTAC TCTGAAGATT
    TACAGGGCGG AAGATCTGCC GCAGATGGAC GATGCTGTGC TGGACAATGT CAAACAGATC
    TTTGGCTTTG ACAGCAACAA GAAAAACTTG GTGGATCCCT TTGTGGAAGT CAGTTTTGCG
    GGGAAGATGC TCCTCAGTAA GATCATGGAG AAGAACGCCA ACCCGCAGTG GAGTCAGTCC
    ATCACTCTTC CCATCAGGTT CCCCTCTATG TGTGAAAAAA TGCGCATCCG CCTGTTGGAC
    TGGGACCGCC TCACCCACAA CGATATCATC GGCACCACCT ACCTGAGCAT GTCCAAAATC
    TCAGCTCCTG GCGGCGAGAT TGAAGATGAG ACACCGCGTA AGGCCTTGCC GGTTTCTAAC
    TTTGATGACA CTTTGGGATT TCTGCCAACC TTCGGGCCTT GCTATGTGAA CCTTTATGGC
    AGCCCTCGGG AGTTCACTGG GTTTCCAGAC CCTTATGAAG ATCTGAACTT GGGGAAGGGA
    GAAGGAGTGG CTTACCGTGG TCGGGTCCTT CTGGAGTTGG AGACAAAGCT GGTGGAGCAG
    GCTGAGCAGA AGGTGGAAGA CATTCCTTCA GATGATATTT CACGGGTTGA GAAATATCTC
    CGCAGACGGA AGTACTGCCT CTTTGCCGCT TTCTATTCAG CCACCATGTT GCAGGACGTC
    GATGACGCCA TTCAGTTTGA GGTCAGCATT GGAAATTATG GAAACAAGTT CGACAACACC
    TGCCTCCCCC TGGCCTCCAC CACGCAGTAC AGCCGGGCTG TGTTCGACGG TTGCCAGTAC
    TATTATCTCC CCTGGGGTAA CGTAAAGCCC GTTGTTGTTC TCTCATCTCA CTGGGAAGAC
    ATCGGCCACA GAACTGACAC TCAGAATATT CTACAATACG CCATCGATAA TCTGGCAGCG
    AATCTGGACT GGGTCCACCT TGCTGTGAAA GCTAAGAGCT CCCCCTACGA CTTGGACGCT
    TTGGTCGCGC GTTTGTTTGA TGACATCATC ACCGACTGCA GCCACGCCCT GCCAGATGTC
    AAGTCTATGC AAGGGACCAC CCACCTGGAC CAGTACTTGT ACAGATTCCG CAAGACCAAC
    CTTGCCCAGA TTGTCCAGGC GGCTCTGAAA CTCAAGCACG ATGACTGTGT GGAGCAGGAG
    AATGTGATGG AGCAGGCAGA GGACTGGCTA ATGCGACTAA AGGCTATGGC TGAGGAGCCG
    CAGAACAGCC TGCCAGACAT AGTGATTTGG ATGTTACAAG GGGAGAAGCG TGTGGCTTAT
    TATAGAATGC CGGCCCGCGA TGTCCTTTTC TCCAGGAATG GCTCCAAGTG TTGCGGCAAG
    AACTGCGGGA AGCTCCAGAC AATATTTCTC AAATTTCCTC AGGAAGGAAC CCAAGGAGCG
    AAGATGCCAG CTCAGGTTCG AGTCCAGCTG TGGTTTGGGC TCGCTGTGGA TCAGAAGGAA
    TTCAATCATT ATGCTCAGGG GAAATTGTCT GTTTTTGCTG AAGCTTATGA AAACCAAACA
    AAGCTGGCTC TCGTGGGCAA CTGGGGAACA ACTGGCCTTA CGTACCCCAA ATACTCAGAC
    GTGACCGGGA AAATCAAACT TCCAAAGGAG AGTTTCAAGG CTTCCACTGG ATGGAGCTGG
    GCCGGAGACT GGTTTATCAG CCCGGAGAAA ACGTTGCTGT ATGATACGGA CGCGGGTCAC
    ATGAGCTTCG TGGAAGAGGT TTTCGAGAAC CAGGCCAGGC TGCCCGGTGG GCAATGGATC
    CATATGACGG AGGCCTACTC AGATGTGAAT GGGGAGAAGG TTTTGCCAAA AGATGAGATC
    GAATGCCCCC CTGGCTGGAA ATGGGAGGAT GATGAATGGT CCACGGACAC CAACAGGGCT
    GTTGATGAGC AAGGATGGGA GTACGGCATT ACTATCCCCC CTGACCGGAA GCCTAAATCC
    TGGGTTCCAG CAGAGAAGAT GTATCACACC AACCGCAGGA GGCGCTGGGT CAGACTAAGA
    CGAAGGGACC CCAAGCAGAT TGAGATCCTA AAAAAGCACA AGCCGGACGA GTCGGAGAGC
    GAGGGATGGG AATACGCCTC TCTCTTTGGC TGGAAGTTTC ACCTGAAGTA CCGTAAGACT
    GACAGTTTCC GCAGGCGCAG GTGGAGGCGC CGTATGGAAC CCCTGGAACG CACCGGGGCG
    GCAGCTGTTT TCGCTTTGGA AGGTTCCCTG GGTGGAATTA CAGATGATAA AAGCGATGAT
    GGAAAAAACA CGTCCACAAT GAGCTTCGGG GTCAACCGGC CGACGATATC CTGCATATTT
    GACTATGGGA ACCGTTACCA TTTGCGCTGC TACATATACC AAGCGAGGAA CCTGGTGCCC
    ATGGACCAAG ACAGCTTTTC TGATCCATAC GCCATAGTCT CTTTCCTCCA TCAAAGTCAA
    AAGACCTTGG TGATGAAGAG CACCTTGAAC CCAACATGGG ACCAGACCCT TATTTTTCAT
    GAAATTGAAG TTTTTGGAGA CCCTGAAACC ATAAAAGAAT CCCCTCCCAA CATAGTGGTG
    GAAATCTATG ACCACGATAC TTACGGTGCA GATGAATACA TGGGAAGGTG CATTTGCAAG
    CCAAGCTTTG AGCGCTCCCC CCGGTTATCT TGGCACCCGG TGATCATGGG CACGAAGAAC
    GTCGGAGAGC TGCTCGCTGC CTTCGAGCTC ATTCCCAGGG AAAAGCCGGC TGTTCATCTT
    ATCCCAGGAC TGGAGGGAGA AATCTCAGGA GTTCTGAGCG AGCTGCTTAT TCCTCGCTTC
    TTCACCGAGA AACTTCTTGA CTGGTCATCA GACGCCGACC TTCCCTACCC ACCTCCACAG
    AGGGAGGCCA ATGTGTACAT GGTGCCCCAG GGAATCAAAC CCGTTCTGCA GCGCACCGCT
    ATAGAGATCC TGGCCTGGGG GCTGAGGAAC CTAAAGAGCT ACCAGCTGGC CAGCGTGACC
    TCCCCGAGTC TGATAGTGGA GTGCGGCGAT GAACTGATCC AGTCGGGGGT CATTAAGAAC
    GTCAAGAAGA ACCCCAACTT TGATGTGTGC CTGCTCCTCA TGGAAGTGAG GTTGCCCAGA
    GAAGATCTAT ATTGTCCACC CATCATTGTG AAGGTCATCG ATAACCGTCA GTTTGGAAGA
    AAACCAGTCG TCGGCCAATG CACCATCAAG TCTTTGGAAG AGTTCCATTG TGACCCCAAC
    TCAGAAGATC TAGAGGTAGA AGATGGACTG TCAGACAATG TGAGTGTTAC ACCCAGGGAC
    GAGGTGCTTA TTGACATTGA TGATAAGGAA CCTCTTATCC CGACTCAGCT TGCCGACGGT
    ATGACCAGCT CAGCCATTAT TAACTTATCA TCTTCTCGGC CCAGTCTCCA TGAAGAAGAG
    TTCATTGATT GGTGGAGTAA GTTTTATGCT TCCATTGGGG AAAAAGAGAA ATGTGGAACA
    TACCTAGAGA AAGGCTTTGA TACCTTGCAG GTACTGGACA CAGAGCTAGA GAACATAGAG
    GATTACGAAG AACTCTCAGA CTTTTGCCGA ACCTTCAAAC TGTACAGAGG AAAAACACAA
    GATGAGACAG AAGATCCCTC TGTGGTTGGA GAATTTAAGG GATCCTTTAA AATCTATCCT
    CTTCCTGATG ACCCATCAGT CCCCAGGCCA GCACCCCAGT TCCGCCAACT CCCTGCCAAA
    GGTCTTCAAG AGTGCCTCGT GCGTGTCTAT ATTGTTCGTG CTTTTGGTTT GCAGCCGAAA
    GACCCCAACG GCAAGTGTGA TCCCTACGTG AAGATTTCCA TTGGAAAGAA GTCATTAAAT
    GATCAGGAAC ATTATATCCC TTGCACCTTG GAGCCTGTTT TTGGAAAAAT GTTTGAGCTG
    ACCTGCACCC TCCCTCTGGA GAAAGACCTG AAGGTCACAC TCTATGATTA TGACATGATT
    TCAAAAGATG AGAAGATTGG AGAGACGGTG ATAGACCTGG AGAACCGTTT CCTCTCGAAA
    TATGGAGCAA GATGTGGATT GCCTCAATCC TACTGCATAT CTGGGCCAAA CCAGTGGCGG
    GACCAACGGA AACCATCGGA ACTTCTCAGA ATCTTTGCGC AGCAGCGGAA CTTCAAACCC
    CCTAACTTCA AAAACAACAG CATAATGTTC CGGAACCATG AGTACCATGT CTCTGAGCTG
    GAAGACAGCA AGTTCCCTAA CCCGCACCAG GGCCCGCCGG AGGAACGGCT CGCTCTACAC
    GTACTGAGAA GGCAAGGCCT GGTGTGTGAG CATGTGGAGA CCAGGGCCCT GTACAGCCCT
    TTGCAGCCGG ATATAGAACA GGGAAAGCTG CAGATGTGGG TTGACTTGTT CCCCAAGTCC
    CTCGGTTCTC CCGGCCCTCC CTTTAATATA ACCCCACGGA AAGCCAAACG GTATTACTTG
    CGCTGTATCA TCTGGAACAC CAAAGATGTG ATTCTGGATG ATGTGAGCAT CACAGGAGAG
    AAGATGAGTG ATATCTATGT CAAGAGCTGG CTGATTGGCC ATGAAGAAAA TAAACAGAAG
    ACAGATGTTC ACTACAGGTC TATGGGAGGT GAAGGGAATT TTAACTGGCG GCTTGTGTTC
    CCTTTCGACT ATCTTCCCGC GGAGCAGGTC TGCTCAGTGT CCAGAAAGGA GCATTTCTGG
    AGCCTCGACA AGACTGAGAA TAAAATCCCT CCAAACCTCA TTATACAGAT ATGGGACAAC
    GATAAGTTCT CCTTCGATGA CTATTTAGGT TCCGTGCAGC TGGACCTCAA CCGGATGCCA
    CGACCTGCCA AAACGGCGGA GAAGTGCAGG CTGGACCTGC TGGATGAGGT GGAGTCTGCA
    AATCACTGCT CCTTGTTTGA GCAGAAGACA GTCAAGGGAT GGTGGCCGTG TGTAGGGCAG
    GAGGGGGACG AGAAGATTCT GGCTGGAAAA GTGGAAATTA CTTTAGAGAT TGTAAATGAA
    CAGGAACATG ATGAGAGACC GGCCGGGATT GGTCGAGATG AGCCAAATAT GAACCCAAGA
    CTTGAAGACC CAAAGCGCCC AGAAACCTCG TTCCTCTGGT TCACTTCCCC GTACAAGACC
    CTGAAGTACA TCCTGTGGAG GAGATACAAG TGGCTCATCC TTGTCTTCTT CCTTCTATTC
    ATCCTACTGC TCTTCCTCGG AATCTTCCTG TATTCCTTCC CGAACTACGC TGCTATGAAG
    CTGGTGAAGC CGTTCAGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa49537
    Clone ID Related Accession (Same CDS sequence) XM_031895264.1
    Accession Version XM_031895264.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6291bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product dysferlin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGCGCT GTGTGGTCCA AAGGGCCAGC AACCTCCCAA ATGTGGAGAA AGGGGTCAGG 
    TACAGTGACC CAGTTACCAG TTTAACCTTC AGAGGGGTTA AGAAGAAAAC GAAAATCATA
    AAGAACAATC TGAATCCCAT ATGGAACGAG GGATTCGAGT GGGATTTAAA AGGATTGCCC
    TTGGACAACA GTTCGGAGCT TGTCGCGGTA GTGAAAGACC ATGAAACCAT GGGAAGGAAC
    AGGTTTCTGG GTGAGACCCG AGTCAGCCTA CGGGATTTAC TTACGTCCCC TAACCTGACG
    GCCACGTATA ATGCCCCACT CCTGGACAGC AAGAAGCAGG GCACGGGGGC GATATTAACC
    TTGCAGATAT CTTACTTTCC TCCACCGGGG GCAGCCCCAG TTGTACCCCC TCCTGCACCC
    AAGGAGCCCA CCCCATCGCT CAATGAGTTG GACACCATCA CTGATACGGC TGGTGAAGAG
    GACACGGAAG ACCAGGCTGG AACTGGCGAT GAAGCGGATA TTGTCTCTCT GGCCCCTGGA
    GGAGCAGAGC AACCCACTCT CCCCAAGAAA ACTGCCCCTG TCCCTGTGCA GGGATTAAAG
    AGAAAGAAGA GCAACTCCAA AAAACACCTC TCCAACAAGC CTCAGGACTT CCAGGTTCGT
    GTTCGAGTGA TAGAAGGGAG GCAGATCCCA GGGATTAACA TCAAACCTGT TGTCAAAGTC
    ACAGTTGCTG GGCAGACCAA GAGAACCCGG ATACGCAAAG GGAACAACCC ATATTTCGAT
    GAGACATTTT TCTTCAATTT TTTTGAGTCC CCTTCTGAAC TCTTTGATGA ACCCGTGTTC
    CTGACTGTTC TCGATTCCCG CTCCCTCCGA ACAGACTCAG TGATTGGTGA ATTCCGGATG
    GACGTCGGAA TGATTTATGG AGAATCAAAA CATGCCTTCC TCAGGAAGTG GCTTCTCCTC
    TCCGACCCAG ACGATTTCTC CGCGGGGGCC AAGGGGTACC TGAAGGTCAG CCTCTATGTA
    CTTGGGCCTG GAGATGAGAG CCCGGTAGAG AAAAAGGAGG TCTCGGAAGA CAAGGAGGAC
    ATTGAGGGGA ACTTGCTGCG ACCGGCCGGG GTGTCCCTGC GAGGAGCCCA CCTTACTCTG
    AAGATTTACA GGGCGGAAGA TCTGCCGCAG ATGGACGATG CTGTGCTGGA CAATGTCAAA
    CAGATCTTTG GCTTTGACAG CAACAAGAAA AACTTGGTGG ATCCCTTTGT GGAAGTCAGT
    TTTGCGGGGA AGATGCTCCT CAGTAAGATC ATGGAGAAGA ACGCCAACCC GCAGTGGAGT
    CAGTCCATCA CTCTTCCCAT CAGGTTCCCC TCTATGTGTG AAAAAATGCG CATCCGCCTG
    TTGGACTGGG ACCGCCTCAC CCACAACGAT ATCATCGGCA CCACCTACCT GAGCATGTCC
    AAAATCTCAG CTCCTGGCGG CGAGATTGAA GTTGATGACA CTTTGGGATT TCTGCCAACC
    TTCGGGCCTT GCTATGTGAA CCTTTATGGC AGCCCTCGGG AGTTCACTGG GTTTCCAGAC
    CCTTATGAAG ATCTGAACTT GGGGAAGGGA GAAGGAGTGG CTTACCGTGG TCGGGTCCTT
    CTGGAGTTGG AGACAAAGCT GGTGGAGCAG GCTGAGCAGA AGGTGGAAGA CATTCCTTCA
    GATGATATTT CACGGGTTGA GAAATATCTC CGCAGACGGA AGTACTGCCT CTTTGCCGCT
    TTCTATTCAG CCACCATGTT GCAGGACGTC GATGACGCCA TTCAGTTTGA GGTCAGCATT
    GGAAATTATG GAAACAAGTT CGACAACACC TGCCTCCCCC TGGCCTCCAC CACGCAGTAC
    AGCCGGGCTG TGTTCGACGG TTGCCAGTAC TATTATCTCC CCTGGGGTAA CGTAAAGCCC
    GTTGTTGTTC TCTCATCTCA CTGGGAAGAC ATCGGCCACA GAACTGACAC TCAGAATATT
    CTACAATACG CCATCGATAA TCTGGCAGCG AATCTGGACT GGGTCCACCT TGCTGTGAAA
    GCTAAGAGCT CCCCCTACGA CTTGGACGCT TTGGTCGCGC GTTTGTTTGA TGACATCATC
    ACCGACTGCA GCCACGCCCT GCCAGATGTC AAGTCTATGC AAGGGACCAC CCACCTGGAC
    CAGTACTTGT ACAGATTCCG CAAGACCAAC CTTGCCCAGA TTGTCCAGGC GGCTCTGAAA
    CTCAAGCACG ATGACTGTGT GGAGCAGGAG AATGTGATGG AGCAGGCAGA GGACTGGCTA
    ATGCGACTAA AGGCTATGGC TGAGGAGCCG CAGAACAGCC TGCCAGACAT AGTGATTTGG
    ATGTTACAAG GGGAGAAGCG TGTGGCTTAT TATAGAATGC CGGCCCGCGA TGTCCTTTTC
    TCCAGGAATG GCTCCAAGTG TTGCGGCAAG AACTGCGGGA AGCTCCAGAC AATATTTCTC
    AAATTTCCTC AGGAAGGAAC CCAAGGAGCG AAGATGCCAG CTCAGGTTCG AGTCCAGCTG
    TGGTTTGGGC TCGCTGTGGA TCAGAAGGAA TTCAATCATT ATGCTCAGGG GAAATTGTCT
    GTTTTTGCTG AAGCTTATGA AAACCAAACA AAGCTGGCTC TCGTGGGCAA CTGGGGAACA
    ACTGGCCTTA CGTACCCCAA ATACTCAGAC GTGACCGGGA AAATCAAACT TCCAAAGGAG
    AGTTTCAAGG CTTCCACTGG ATGGAGCTGG GCCGGAGACT GGTTTATCAG CCCGGAGAAA
    ACGTTGCTGT ATGATACGGA CGCGGGTCAC ATGAGCTTCG TGGAAGAGGT TTTCGAGAAC
    CAGGCCAGGC TGCCCGGTGG GCAATGGATC CATATGACGG AGGCCTACTC AGATGTGAAT
    GGGGAGAAGG TTTTGCCAAA AGATGAGATC GAATGCCCCC CTGGCTGGAA ATGGGAGGAT
    GATGAATGGT CCACGGACAC CAACAGGGCT GTTGATGAGC AAGGATGGGA GTACGGCATT
    ACTATCCCCC CTGACCGGAA GCCTAAATCC TGGGTTCCAG CAGAGAAGAT GTATCACACC
    AACCGCAGGA GGCGCTGGGT CAGACTAAGA CGAAGGGACC CCAAGCAGAT TGAGATCCTA
    AAAAAGCACA AGCCGGACGA GTCGGAGAGC GAGGGATGGG AATACGCCTC TCTCTTTGGC
    TGGAAGTTTC ACCTGAAGTA CCGTAAGACT GACAGTTTCC GCAGGCGCAG GTGGAGGCGC
    CGTATGGAAC CCCTGGAACG CACCGGGGCG GCAGCTGTTT TCGCTTTGGA AGGTTCCCTG
    GGTGGAATTA CAGATGATAA AAGCGATGAT GGAAAAAACA CGTCCACAAT GAGCTTCGGG
    GTCAACCGGC CGACGATATC CTGCATATTT GACTATGGGA ACCGTTACCA TTTGCGCTGC
    TACATATACC AAGCGAGGAA CCTGGTGCCC ATGGACCAAG ACAGCTTTTC TGATCCATAC
    GCCATAGTCT CTTTCCTCCA TCAAAGTCAA AAGACCTTGG TGATGAAGAG CACCTTGAAC
    CCAACATGGG ACCAGACCCT TATTTTTCAT GAAATTGAAG TTTTTGGAGA CCCTGAAACC
    ATAAAAGAAT CCCCTCCCAA CATAGTGGTG GAAATCTATG ACCACGATAC TTACGGTGCA
    GATGAATACA TGGGAAGGTG CATTTGCAAG CCAAGCTTTG AGCGCTCCCC CCGGTTATCT
    TGGCACCCGG TGATCATGGG CACGAAGAAC GTCGGAGAGC TGCTCGCTGC CTTCGAGCTC
    ATTCCCAGGG AAAAGCCGGC TGTTCATCTT ATCCCAGGAC TGGAGGGAGA AATCTCAGGA
    GTTCTGAGCG AGCTGCTTAT TCCTCGCTTC TTCACCGAGA AACTTCTTGA CTGGTCATCA
    GACGCCGACC TTCCCTACCC ACCTCCACAG AGGGAGGCCA ATGTGTACAT GGTGCCCCAG
    GGAATCAAAC CCGTTCTGCA GCGCACCGCT ATAGAGATCC TGGCCTGGGG GCTGAGGAAC
    CTAAAGAGCT ACCAGCTGGC CAGCGTGACC TCCCCGAGTC TGATAGTGGA GTGCGGCGAT
    GAACTGATCC AGTCGGGGGT CATTAAGAAC GTCAAGAAGA ACCCCAACTT TGATGTGTGC
    CTGCTCCTCA TGGAAGTGAG GTTGCCCAGA GAAGATCTAT ATTGTCCACC CATCATTGTG
    AAGGTCATCG ATAACCGTCA GTTTGGAAGA AAACCAGTCG TCGGCCAATG CACCATCAAG
    TCTTTGGAAG AGTTCCATTG TGACCCCAAC TCAGAAGATC TAGAGGTAGA AGATGGACTG
    TCAGACAATG TGAGTGTTAC ACCCAGGGAC GAGGTGCTTA TTGACATTGA TGATAAGGAA
    CCTCTTATCC CGACTCAGCT TGCCGACGGT ATGACCAGCT CAGCCATTAT TAACTTATCA
    TCTTCTCGGC CCAGTCTCCA TGAAGAAGAG TTCATTGATT GGTGGAGTAA GTTTTATGCT
    TCCATTGGGG AAAAAGAGAA ATGTGGAACA TACCTAGAGA AAGGCTTTGA TACCTTGCAG
    GTACTGGACA CAGAGCTAGA GAACATAGAG GATTACGAAG AACTCTCAGA CTTTTGCCGA
    ACCTTCAAAC TGTACAGAGG AAAAACACAA GATGAGACAG AAGATCCCTC TGTGGTTGGA
    GAATTTAAGG GATCCTTTAA AATCTATCCT CTTCCTGATG ACCCATCAGT CCCCAGGCCA
    GCACCCCAGT TCCGCCAACT CCCTGCCAAA GGTCTTCAAG AGTGCCTCGT GCGTGTCTAT
    ATTGTTCGTG CTTTTGGTTT GCAGCCGAAA GACCCCAACG GCAAGTGTGA TCCCTACGTG
    AAGATTTCCA TTGGAAAGAA GTCATTAAAT GATCAGGAAC ATTATATCCC TTGCACCTTG
    GAGCCTGTTT TTGGAAAAAT GTTTGAGCTG ACCTGCACCC TCCCTCTGGA GAAAGACCTG
    AAGGTCACAC TCTATGATTA TGACATGATT TCAAAAGATG AGAAGATTGG AGAGACGGTG
    ATAGACCTGG AGAACCGTTT CCTCTCGAAA TATGGAGCAA GATGTGGATT GCCTCAATCC
    TACTGCATAT CTGGGCCAAA CCAGTGGCGG GACCAACGGA AACCATCGGA ACTTCTCAGA
    ATCTTTGCGC AGCAGCGGAA CTTCAAACCC CCTAACTTCA AAAACAACAG CATAATGTTC
    CGGAACCATG AGTACCATGT CTCTGAGCTG GAAGACAGCA AGTTCCCTAA CCCGCACCAG
    GGCCCGCCGG AGGAACGGCT CGCTCTACAC GTACTGAGAA GGCAAGGCCT GGTGTGTGAG
    CATGTGGAGA CCAGGGCCCT GTACAGCCCT TTGCAGCCGG ATATAGAACA GGGAAAGCTG
    CAGATGTGGG TTGACTTGTT CCCCAAGTCC CTCGGTTCTC CCGGCCCTCC CTTTAATATA
    ACCCCACGGA AAGCCAAACG GTATTACTTG CGCTGTATCA TCTGGAACAC CAAAGATGTG
    ATTCTGGATG ATGTGAGCAT CACAGGAGAG AAGATGAGTG ATATCTATGT CAAGAGCTGG
    CTGATTGGCC ATGAAGAAAA TAAACAGAAG ACAGATGTTC ACTACAGGTC TATGGGAGGT
    GAAGGGAATT TTAACTGGCG GCTTGTGTTC CCTTTCGACT ATCTTCCCGC GGAGCAGGTC
    TGCTCAGTGT CCAGAAAGGA GCATTTCTGG AGCCTCGACA AGACTGAGAA TAAAATCCCT
    CCAAACCTCA TTATACAGAT ATGGGACAAC GATAAGTTCT CCTTCGATGA CTATTTAGGT
    TCCGTGCAGC TGGACCTCAA CCGGATGCCA CGACCTGCCA AAACGGCGGA GAAGTGCAGG
    CTGGACCTGC TGGATGAGGT GGAGTCTGCA AATCACTGCT CCTTGTTTGA GCAGAAGACA
    GTCAAGGGAT GGTGGCCGTG TGTAGGGCAG GAGGGGGACG AGAAGATTCT GGCTGGAAAA
    GTGGAAATTA CTTTAGAGAT TGTAAATGAA CAGGAACATG ATGAGAGACC GGCCGGGATT
    GGTCGAGATG AGCCAAATAT GAACCCAAGA CTTGAAGACC CAAAGCGCCC AGAAACCTCG
    TTCCTCTGGT TCACTTCCCC GTACAAGACC CTGAAGTACA TCCTGTGGAG GAGATACAAG
    TGGCTCATCC TTGTCTTCTT CCTTCTATTC ATCCTACTGC TCTTCCTCGG AATCTTCCTG
    TATTCCTTCC CGAACTACGC TGCTATGAAG CTGGTGAAGC CGTTCAGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751124.1
    CDS1273..7563
    Translation

    Target ORF information:

    RefSeq Version XM_031895264.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis dysferlin (LOC100488564), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031895264.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGCGCT GTGTGGTCCA AAGGGCCAGC AACCTCCCAA ATGTGGAGAA AGGGGTCAGG 
    TACAGTGACC CAGTTACCAG TTTAACCTTC AGAGGGGTTA AGAAGAAAAC GAAAATCATA
    AAGAACAATC TGAATCCCAT ATGGAACGAG GGATTCGAGT GGGATTTAAA AGGATTGCCC
    TTGGACAACA GTTCGGAGCT TGTCGCGGTA GTGAAAGACC ATGAAACCAT GGGAAGGAAC
    AGGTTTCTGG GTGAGACCCG AGTCAGCCTA CGGGATTTAC TTACGTCCCC TAACCTGACG
    GCCACGTATA ATGCCCCACT CCTGGACAGC AAGAAGCAGG GCACGGGGGC GATATTAACC
    TTGCAGATAT CTTACTTTCC TCCACCGGGG GCAGCCCCAG TTGTACCCCC TCCTGCACCC
    AAGGAGCCCA CCCCATCGCT CAATGAGTTG GACACCATCA CTGATACGGC TGGTGAAGAG
    GACACGGAAG ACCAGGCTGG AACTGGCGAT GAAGCGGATA TTGTCTCTCT GGCCCCTGGA
    GGAGCAGAGC AACCCACTCT CCCCAAGAAA ACTGCCCCTG TCCCTGTGCA GGGATTAAAG
    AGAAAGAAGA GCAACTCCAA AAAACACCTC TCCAACAAGC CTCAGGACTT CCAGGTTCGT
    GTTCGAGTGA TAGAAGGGAG GCAGATCCCA GGGATTAACA TCAAACCTGT TGTCAAAGTC
    ACAGTTGCTG GGCAGACCAA GAGAACCCGG ATACGCAAAG GGAACAACCC ATATTTCGAT
    GAGACATTTT TCTTCAATTT TTTTGAGTCC CCTTCTGAAC TCTTTGATGA ACCCGTGTTC
    CTGACTGTTC TCGATTCCCG CTCCCTCCGA ACAGACTCAG TGATTGGTGA ATTCCGGATG
    GACGTCGGAA TGATTTATGG AGAATCAAAA CATGCCTTCC TCAGGAAGTG GCTTCTCCTC
    TCCGACCCAG ACGATTTCTC CGCGGGGGCC AAGGGGTACC TGAAGGTCAG CCTCTATGTA
    CTTGGGCCTG GAGATGAGAG CCCGGTAGAG AAAAAGGAGG TCTCGGAAGA CAAGGAGGAC
    ATTGAGGGGA ACTTGCTGCG ACCGGCCGGG GTGTCCCTGC GAGGAGCCCA CCTTACTCTG
    AAGATTTACA GGGCGGAAGA TCTGCCGCAG ATGGACGATG CTGTGCTGGA CAATGTCAAA
    CAGATCTTTG GCTTTGACAG CAACAAGAAA AACTTGGTGG ATCCCTTTGT GGAAGTCAGT
    TTTGCGGGGA AGATGCTCCT CAGTAAGATC ATGGAGAAGA ACGCCAACCC GCAGTGGAGT
    CAGTCCATCA CTCTTCCCAT CAGGTTCCCC TCTATGTGTG AAAAAATGCG CATCCGCCTG
    TTGGACTGGG ACCGCCTCAC CCACAACGAT ATCATCGGCA CCACCTACCT GAGCATGTCC
    AAAATCTCAG CTCCTGGCGG CGAGATTGAA GTTGATGACA CTTTGGGATT TCTGCCAACC
    TTCGGGCCTT GCTATGTGAA CCTTTATGGC AGCCCTCGGG AGTTCACTGG GTTTCCAGAC
    CCTTATGAAG ATCTGAACTT GGGGAAGGGA GAAGGAGTGG CTTACCGTGG TCGGGTCCTT
    CTGGAGTTGG AGACAAAGCT GGTGGAGCAG GCTGAGCAGA AGGTGGAAGA CATTCCTTCA
    GATGATATTT CACGGGTTGA GAAATATCTC CGCAGACGGA AGTACTGCCT CTTTGCCGCT
    TTCTATTCAG CCACCATGTT GCAGGACGTC GATGACGCCA TTCAGTTTGA GGTCAGCATT
    GGAAATTATG GAAACAAGTT CGACAACACC TGCCTCCCCC TGGCCTCCAC CACGCAGTAC
    AGCCGGGCTG TGTTCGACGG TTGCCAGTAC TATTATCTCC CCTGGGGTAA CGTAAAGCCC
    GTTGTTGTTC TCTCATCTCA CTGGGAAGAC ATCGGCCACA GAACTGACAC TCAGAATATT
    CTACAATACG CCATCGATAA TCTGGCAGCG AATCTGGACT GGGTCCACCT TGCTGTGAAA
    GCTAAGAGCT CCCCCTACGA CTTGGACGCT TTGGTCGCGC GTTTGTTTGA TGACATCATC
    ACCGACTGCA GCCACGCCCT GCCAGATGTC AAGTCTATGC AAGGGACCAC CCACCTGGAC
    CAGTACTTGT ACAGATTCCG CAAGACCAAC CTTGCCCAGA TTGTCCAGGC GGCTCTGAAA
    CTCAAGCACG ATGACTGTGT GGAGCAGGAG AATGTGATGG AGCAGGCAGA GGACTGGCTA
    ATGCGACTAA AGGCTATGGC TGAGGAGCCG CAGAACAGCC TGCCAGACAT AGTGATTTGG
    ATGTTACAAG GGGAGAAGCG TGTGGCTTAT TATAGAATGC CGGCCCGCGA TGTCCTTTTC
    TCCAGGAATG GCTCCAAGTG TTGCGGCAAG AACTGCGGGA AGCTCCAGAC AATATTTCTC
    AAATTTCCTC AGGAAGGAAC CCAAGGAGCG AAGATGCCAG CTCAGGTTCG AGTCCAGCTG
    TGGTTTGGGC TCGCTGTGGA TCAGAAGGAA TTCAATCATT ATGCTCAGGG GAAATTGTCT
    GTTTTTGCTG AAGCTTATGA AAACCAAACA AAGCTGGCTC TCGTGGGCAA CTGGGGAACA
    ACTGGCCTTA CGTACCCCAA ATACTCAGAC GTGACCGGGA AAATCAAACT TCCAAAGGAG
    AGTTTCAAGG CTTCCACTGG ATGGAGCTGG GCCGGAGACT GGTTTATCAG CCCGGAGAAA
    ACGTTGCTGT ATGATACGGA CGCGGGTCAC ATGAGCTTCG TGGAAGAGGT TTTCGAGAAC
    CAGGCCAGGC TGCCCGGTGG GCAATGGATC CATATGACGG AGGCCTACTC AGATGTGAAT
    GGGGAGAAGG TTTTGCCAAA AGATGAGATC GAATGCCCCC CTGGCTGGAA ATGGGAGGAT
    GATGAATGGT CCACGGACAC CAACAGGGCT GTTGATGAGC AAGGATGGGA GTACGGCATT
    ACTATCCCCC CTGACCGGAA GCCTAAATCC TGGGTTCCAG CAGAGAAGAT GTATCACACC
    AACCGCAGGA GGCGCTGGGT CAGACTAAGA CGAAGGGACC CCAAGCAGAT TGAGATCCTA
    AAAAAGCACA AGCCGGACGA GTCGGAGAGC GAGGGATGGG AATACGCCTC TCTCTTTGGC
    TGGAAGTTTC ACCTGAAGTA CCGTAAGACT GACAGTTTCC GCAGGCGCAG GTGGAGGCGC
    CGTATGGAAC CCCTGGAACG CACCGGGGCG GCAGCTGTTT TCGCTTTGGA AGGTTCCCTG
    GGTGGAATTA CAGATGATAA AAGCGATGAT GGAAAAAACA CGTCCACAAT GAGCTTCGGG
    GTCAACCGGC CGACGATATC CTGCATATTT GACTATGGGA ACCGTTACCA TTTGCGCTGC
    TACATATACC AAGCGAGGAA CCTGGTGCCC ATGGACCAAG ACAGCTTTTC TGATCCATAC
    GCCATAGTCT CTTTCCTCCA TCAAAGTCAA AAGACCTTGG TGATGAAGAG CACCTTGAAC
    CCAACATGGG ACCAGACCCT TATTTTTCAT GAAATTGAAG TTTTTGGAGA CCCTGAAACC
    ATAAAAGAAT CCCCTCCCAA CATAGTGGTG GAAATCTATG ACCACGATAC TTACGGTGCA
    GATGAATACA TGGGAAGGTG CATTTGCAAG CCAAGCTTTG AGCGCTCCCC CCGGTTATCT
    TGGCACCCGG TGATCATGGG CACGAAGAAC GTCGGAGAGC TGCTCGCTGC CTTCGAGCTC
    ATTCCCAGGG AAAAGCCGGC TGTTCATCTT ATCCCAGGAC TGGAGGGAGA AATCTCAGGA
    GTTCTGAGCG AGCTGCTTAT TCCTCGCTTC TTCACCGAGA AACTTCTTGA CTGGTCATCA
    GACGCCGACC TTCCCTACCC ACCTCCACAG AGGGAGGCCA ATGTGTACAT GGTGCCCCAG
    GGAATCAAAC CCGTTCTGCA GCGCACCGCT ATAGAGATCC TGGCCTGGGG GCTGAGGAAC
    CTAAAGAGCT ACCAGCTGGC CAGCGTGACC TCCCCGAGTC TGATAGTGGA GTGCGGCGAT
    GAACTGATCC AGTCGGGGGT CATTAAGAAC GTCAAGAAGA ACCCCAACTT TGATGTGTGC
    CTGCTCCTCA TGGAAGTGAG GTTGCCCAGA GAAGATCTAT ATTGTCCACC CATCATTGTG
    AAGGTCATCG ATAACCGTCA GTTTGGAAGA AAACCAGTCG TCGGCCAATG CACCATCAAG
    TCTTTGGAAG AGTTCCATTG TGACCCCAAC TCAGAAGATC TAGAGGTAGA AGATGGACTG
    TCAGACAATG TGAGTGTTAC ACCCAGGGAC GAGGTGCTTA TTGACATTGA TGATAAGGAA
    CCTCTTATCC CGACTCAGCT TGCCGACGGT ATGACCAGCT CAGCCATTAT TAACTTATCA
    TCTTCTCGGC CCAGTCTCCA TGAAGAAGAG TTCATTGATT GGTGGAGTAA GTTTTATGCT
    TCCATTGGGG AAAAAGAGAA ATGTGGAACA TACCTAGAGA AAGGCTTTGA TACCTTGCAG
    GTACTGGACA CAGAGCTAGA GAACATAGAG GATTACGAAG AACTCTCAGA CTTTTGCCGA
    ACCTTCAAAC TGTACAGAGG AAAAACACAA GATGAGACAG AAGATCCCTC TGTGGTTGGA
    GAATTTAAGG GATCCTTTAA AATCTATCCT CTTCCTGATG ACCCATCAGT CCCCAGGCCA
    GCACCCCAGT TCCGCCAACT CCCTGCCAAA GGTCTTCAAG AGTGCCTCGT GCGTGTCTAT
    ATTGTTCGTG CTTTTGGTTT GCAGCCGAAA GACCCCAACG GCAAGTGTGA TCCCTACGTG
    AAGATTTCCA TTGGAAAGAA GTCATTAAAT GATCAGGAAC ATTATATCCC TTGCACCTTG
    GAGCCTGTTT TTGGAAAAAT GTTTGAGCTG ACCTGCACCC TCCCTCTGGA GAAAGACCTG
    AAGGTCACAC TCTATGATTA TGACATGATT TCAAAAGATG AGAAGATTGG AGAGACGGTG
    ATAGACCTGG AGAACCGTTT CCTCTCGAAA TATGGAGCAA GATGTGGATT GCCTCAATCC
    TACTGCATAT CTGGGCCAAA CCAGTGGCGG GACCAACGGA AACCATCGGA ACTTCTCAGA
    ATCTTTGCGC AGCAGCGGAA CTTCAAACCC CCTAACTTCA AAAACAACAG CATAATGTTC
    CGGAACCATG AGTACCATGT CTCTGAGCTG GAAGACAGCA AGTTCCCTAA CCCGCACCAG
    GGCCCGCCGG AGGAACGGCT CGCTCTACAC GTACTGAGAA GGCAAGGCCT GGTGTGTGAG
    CATGTGGAGA CCAGGGCCCT GTACAGCCCT TTGCAGCCGG ATATAGAACA GGGAAAGCTG
    CAGATGTGGG TTGACTTGTT CCCCAAGTCC CTCGGTTCTC CCGGCCCTCC CTTTAATATA
    ACCCCACGGA AAGCCAAACG GTATTACTTG CGCTGTATCA TCTGGAACAC CAAAGATGTG
    ATTCTGGATG ATGTGAGCAT CACAGGAGAG AAGATGAGTG ATATCTATGT CAAGAGCTGG
    CTGATTGGCC ATGAAGAAAA TAAACAGAAG ACAGATGTTC ACTACAGGTC TATGGGAGGT
    GAAGGGAATT TTAACTGGCG GCTTGTGTTC CCTTTCGACT ATCTTCCCGC GGAGCAGGTC
    TGCTCAGTGT CCAGAAAGGA GCATTTCTGG AGCCTCGACA AGACTGAGAA TAAAATCCCT
    CCAAACCTCA TTATACAGAT ATGGGACAAC GATAAGTTCT CCTTCGATGA CTATTTAGGT
    TCCGTGCAGC TGGACCTCAA CCGGATGCCA CGACCTGCCA AAACGGCGGA GAAGTGCAGG
    CTGGACCTGC TGGATGAGGT GGAGTCTGCA AATCACTGCT CCTTGTTTGA GCAGAAGACA
    GTCAAGGGAT GGTGGCCGTG TGTAGGGCAG GAGGGGGACG AGAAGATTCT GGCTGGAAAA
    GTGGAAATTA CTTTAGAGAT TGTAAATGAA CAGGAACATG ATGAGAGACC GGCCGGGATT
    GGTCGAGATG AGCCAAATAT GAACCCAAGA CTTGAAGACC CAAAGCGCCC AGAAACCTCG
    TTCCTCTGGT TCACTTCCCC GTACAAGACC CTGAAGTACA TCCTGTGGAG GAGATACAAG
    TGGCTCATCC TTGTCTTCTT CCTTCTATTC ATCCTACTGC TCTTCCTCGG AATCTTCCTG
    TATTCCTTCC CGAACTACGC TGCTATGAAG CTGGTGAAGC CGTTCAGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa49538
    Clone ID Related Accession (Same CDS sequence) XM_031895265.1
    Accession Version XM_031895265.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6285bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product dysferlin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGCGCT GTGTGGTCCA AAGGGCCAGC AACCTCCCAA ATGTGGAGAA AGGGGTCAGG 
    TACAGTGACC CAGTTACCAG TTTAACCTTC AGAGGGGTTA AGAAGAAAAC GAAAATCATA
    AAGAACAATC TGAATCCCAT ATGGAACGAG GGATTCGAGT GGGATTTAAA AGGATTGCCC
    TTGGACAACA GTTCGGAGCT TGTCGCGGTA GTGAAAGACC ATGAAACCAT GGGAAGGAAC
    AGGTTTCTGG GTGAGACCCG AGTCAGCCTA CGGGATTTAC TTACGTCCCC TAACCTGACG
    GCCACGTATA ATGCCCCACT CCTGGACAGC AAGAAGCAGG GCACGGGGGC GATATTAACC
    TTGCAGATAT CTTACTTTCC TCCACCGGGG GCAGCCCCAG TTGTACCCCC TCCTGCACCC
    AAGGAGCCCA CCCCATCGCT CAATGAGTTG GACACCATCA CTGATACGGC TGGTGAAGAG
    GACACGGAAG ACCAGGCTGG AACTGGCGAT GAAGCGGATA TTGTCTCTCT GGCCCCTGGA
    GGAGCAGAGC AACCCACTCT CCCCAAGAAA ACTGCCCCTG TCCCTGTGCA GGGATTAAAG
    AGAAAGAAGA GCAACTCCAA AAAACACCTC TCCAACAAGC CTCAGGACTT CCAGGTTCGT
    GTTCGAGTGA TAGAAGGGAG GCAGATCCCA GGGATTAACA TCAAACCTGT TGTCAAAGTC
    ACAGTTGCTG GGCAGACCAA GAGAACCCGG ATACGCAAAG GGAACAACCC ATATTTCGAT
    GAGACATTTT TCTTCAATTT TTTTGAGTCC CCTTCTGAAC TCTTTGATGA ACCCGTGTTC
    CTGACTGTTC TCGATTCCCG CTCCCTCCGA ACAGACTCAG TGATTGGTGA ATTCCGGATG
    GACGTCGGAA TGATTTATGG AGAATCAAAA CATGCCTTCC TCAGGAAGTG GCTTCTCCTC
    TCCGACCCAG ACGATTTCTC CGCGGGGGCC AAGGGGTACC TGAAGGTCAG CCTCTATGTA
    CTTGGGCCTG GAGATGAGAG CCCGGTAGAG AAAAAGGAGG TCTCGGAAGA CAAGGAGGAC
    ATTGAGGGGA ACTTGCTGCG ACCGGCCGGG GTGTCCCTGC GAGGAGCCCA CCTTACTCTG
    AAGATTTACA GGGCGGAAGA TCTGCCGCAG ATGGACGATG CTGTGCTGGA CAATGTCAAA
    CAGATCTTTG GCTTTGACAG CAACAAGAAA AACTTGGTGG ATCCCTTTGT GGAAGTCAGT
    TTTGCGGGGA AGATGCTCCT CAGTAAGATC ATGGAGAAGA ACGCCAACCC GCAGTGGAGT
    CAGTCCATCA CTCTTCCCAT CAGGTTCCCC TCTATGTGTG AAAAAATGCG CATCCGCCTG
    TTGGACTGGG ACCGCCTCAC CCACAACGAT ATCATCGGCA CCACCTACCT GAGCATGTCC
    AAAATCTCAG CTCCTGGCGG CGAGATTGAA GATGAGACAC CGCGTAAGGC CTTGCCGGTT
    TCTAACTTTG ATGACACTTT GGGATTTCTG CCAACCTTCG GGCCTTGCTA TGTGAACCTT
    TATGGCAGCC CTCGGGAGTT CACTGGGTTT CCAGACCCTT ATGAAGATCT GAACTTGGGG
    AAGGGAGAAG GAGTGGCTTA CCGTGGTCGG GTCCTTCTGG AGTTGGAGAC AAAGCTGGTG
    GAGCAGGCTG AGCAGAAGGT GGAAGACATT CCTTCAGATG ATATTTCACG GGTTGAGAAA
    TATCTCCGCA GACGGAAGTA CTGCCTCTTT GCCGCTTTCT ATTCAGCCAC CATGTTGCAG
    GACGTCGATG ACGCCATTCA GTTTGAGGTC AGCATTGGAA ATTATGGAAA CAAGTTCGAC
    AACACCTGCC TCCCCCTGGC CTCCACCACG CAGTACAGCC GGGCTGTGTT CGACGGTTGC
    CAGTACTATT ATCTCCCCTG GGGTAACGTA AAGCCCGTTG TTGTTCTCTC ATCTCACTGG
    GAAGACATCG GCCACAGAAC TGACACTCAG AATATTCTAC AATACGCCAT CGATAATCTG
    GCAGCGAATC TGGACTGGGT CCACCTTGCT GTGAAAGCTA AGAGCTCCCC CTACGACTTG
    GACGCTTTGG TCGCGCGTTT GTTTGATGAC ATCATCACCG ACTGCAGCCA CGCCCTGCCA
    GATGTCAAGT CTATGCAAGG GACCACCCAC CTGGACCAGT ACTTGTACAG ATTCCGCAAG
    ACCAACCTTG CCCAGATTGT CCAGGCGGCT CTGAAACTCA AGCACGATGA CTGTGTGGAG
    CAGGAGAATG TGATGGAGCA GGCAGAGGAC TGGCTAATGC GACTAAAGGC TATGGCTGAG
    GAGCCGCAGA ACAGCCTGCC AGACATAGTG ATTTGGATGT TACAAGGGGA GAAGCGTGTG
    GCTTATTATA GAATGCCGGC CCGCGATGTC CTTTTCTCCA GGAATGGCTC CAAGTGTTGC
    GGCAAGAACT GCGGGAAGCT CCAGACAATA TTTCTCAAAT TTCCTCAGGA AGGAACCCAA
    GGAGCGAAGA TGCCAGCTCA GGTTCGAGTC CAGCTGTGGT TTGGGCTCGC TGTGGATCAG
    AAGGAATTCA ATCATTATGC TCAGGGGAAA TTGTCTGTTT TTGCTGAAGC TTATGAAAAC
    CAAACAAAGC TGGCTCTCGT GGGCAACTGG GGAACAACTG GCCTTACGTA CCCCAAATAC
    TCAGACGTGA CCGGGAAAAT CAAACTTCCA AAGGAGAGTT TCAAGGCTTC CACTGGATGG
    AGCTGGGCCG GAGACTGGTT TATCAGCCCG GAGAAAACGT TGCTGTATGA TACGGACGCG
    GGTCACATGA GCTTCGTGGA AGAGGTTTTC GAGAACCAGG CCAGGCTGCC CGGTGGGCAA
    TGGATCCATA TGACGGAGGC CTACTCAGAT GTGAATGGGG AGAAGGTTTT GCCAAAAGAT
    GAGATCGAAT GCCCCCCTGG CTGGAAATGG GAGGATGATG AATGGTCCAC GGACACCAAC
    AGGGCTGTTG ATGAGCAAGG ATGGGAGTAC GGCATTACTA TCCCCCCTGA CCGGAAGCCT
    AAATCCTGGG TTCCAGCAGA GAAGATGTAT CACACCAACC GCAGGAGGCG CTGGGTCAGA
    CTAAGACGAA GGGACCCCAA GCAGATTGAG ATCCTAAAAA AGCACAAGCC GGACGAGTCG
    GAGAGCGAGG GATGGGAATA CGCCTCTCTC TTTGGCTGGA AGTTTCACCT GAAGTACCGT
    AAGACTGACA GTTTCCGCAG GCGCAGGTGG AGGCGCCGTA TGGAACCCCT GGAACGCACC
    GGGGCGGCAG CTGTTTTCGC TTTGGAAGGT TCCCTGGGTG GAATTACAGA TGATAAAAGC
    GATGATGGAA AAAACACGTC CACAATGAGC TTCGGGGTCA ACCGGCCGAC GATATCCTGC
    ATATTTGACT ATGGGAACCG TTACCATTTG CGCTGCTACA TATACCAAGC GAGGAACCTG
    GTGCCCATGG ACCAAGACAG CTTTTCTGAT CCATACGCCA TAGTCTCTTT CCTCCATCAA
    AGTCAAAAGA CCTTGGTGAT GAAGAGCACC TTGAACCCAA CATGGGACCA GACCCTTATT
    TTTCATGAAA TTGAAGTTTT TGGAGACCCT GAAACCATAA AAGAATCCCC TCCCAACATA
    GTGGTGGAAA TCTATGACCA CGATACTTAC GGTGCAGATG AATACATGGG AAGGTGCATT
    TGCAAGCCAA GCTTTGAGCG CTCCCCCCGG TTATCTTGGC ACCCGGTGAT CATGGGCACG
    AAGAACGTCG GAGAGCTGCT CGCTGCCTTC GAGCTCATTC CCAGGGAAAA GCCGGCTGTT
    CATCTTATCC CAGGACTGGA GGGAGAAATC TCAGGAGTTC TGAGCGAGTC ATCAGACGCC
    GACCTTCCCT ACCCACCTCC ACAGAGGGAG GCCAATGTGT ACATGGTGCC CCAGGGAATC
    AAACCCGTTC TGCAGCGCAC CGCTATAGAG ATCCTGGCCT GGGGGCTGAG GAACCTAAAG
    AGCTACCAGC TGGCCAGCGT GACCTCCCCG AGTCTGATAG TGGAGTGCGG CGATGAACTG
    ATCCAGTCGG GGGTCATTAA GAACGTCAAG AAGAACCCCA ACTTTGATGT GTGCCTGCTC
    CTCATGGAAG TGAGGTTGCC CAGAGAAGAT CTATATTGTC CACCCATCAT TGTGAAGGTC
    ATCGATAACC GTCAGTTTGG AAGAAAACCA GTCGTCGGCC AATGCACCAT CAAGTCTTTG
    GAAGAGTTCC ATTGTGACCC CAACTCAGAA GATCTAGAGG TAGAAGATGG ACTGTCAGAC
    AATGTGAGTG TTACACCCAG GGACGAGGTG CTTATTGACA TTGATGATAA GGAACCTCTT
    ATCCCGACTC AGCTTGCCGA CGGTATGACC AGCTCAGCCA TTATTAACTT ATCATCTTCT
    CGGCCCAGTC TCCATGAAGA AGAGTTCATT GATTGGTGGA GTAAGTTTTA TGCTTCCATT
    GGGGAAAAAG AGAAATGTGG AACATACCTA GAGAAAGGCT TTGATACCTT GCAGGTACTG
    GACACAGAGC TAGAGAACAT AGAGGATTAC GAAGAACTCT CAGACTTTTG CCGAACCTTC
    AAACTGTACA GAGGAAAAAC ACAAGATGAG ACAGAAGATC CCTCTGTGGT TGGAGAATTT
    AAGGGATCCT TTAAAATCTA TCCTCTTCCT GATGACCCAT CAGTCCCCAG GCCAGCACCC
    CAGTTCCGCC AACTCCCTGC CAAAGGTCTT CAAGAGTGCC TCGTGCGTGT CTATATTGTT
    CGTGCTTTTG GTTTGCAGCC GAAAGACCCC AACGGCAAGT GTGATCCCTA CGTGAAGATT
    TCCATTGGAA AGAAGTCATT AAATGATCAG GAACATTATA TCCCTTGCAC CTTGGAGCCT
    GTTTTTGGAA AAATGTTTGA GCTGACCTGC ACCCTCCCTC TGGAGAAAGA CCTGAAGGTC
    ACACTCTATG ATTATGACAT GATTTCAAAA GATGAGAAGA TTGGAGAGAC GGTGATAGAC
    CTGGAGAACC GTTTCCTCTC GAAATATGGA GCAAGATGTG GATTGCCTCA ATCCTACTGC
    ATATCTGGGC CAAACCAGTG GCGGGACCAA CGGAAACCAT CGGAACTTCT CAGAATCTTT
    GCGCAGCAGC GGAACTTCAA ACCCCCTAAC TTCAAAAACA ACAGCATAAT GTTCCGGAAC
    CATGAGTACC ATGTCTCTGA GCTGGAAGAC AGCAAGTTCC CTAACCCGCA CCAGGGCCCG
    CCGGAGGAAC GGCTCGCTCT ACACGTACTG AGAAGGCAAG GCCTGGTGTG TGAGCATGTG
    GAGACCAGGG CCCTGTACAG CCCTTTGCAG CCGGATATAG AACAGGGAAA GCTGCAGATG
    TGGGTTGACT TGTTCCCCAA GTCCCTCGGT TCTCCCGGCC CTCCCTTTAA TATAACCCCA
    CGGAAAGCCA AACGGTATTA CTTGCGCTGT ATCATCTGGA ACACCAAAGA TGTGATTCTG
    GATGATGTGA GCATCACAGG AGAGAAGATG AGTGATATCT ATGTCAAGAG CTGGCTGATT
    GGCCATGAAG AAAATAAACA GAAGACAGAT GTTCACTACA GGTCTATGGG AGGTGAAGGG
    AATTTTAACT GGCGGCTTGT GTTCCCTTTC GACTATCTTC CCGCGGAGCA GGTCTGCTCA
    GTGTCCAGAA AGGAGCATTT CTGGAGCCTC GACAAGACTG AGAATAAAAT CCCTCCAAAC
    CTCATTATAC AGATATGGGA CAACGATAAG TTCTCCTTCG ATGACTATTT AGGTTCCGTG
    CAGCTGGACC TCAACCGGAT GCCACGACCT GCCAAAACGG CGGAGAAGTG CAGGCTGGAC
    CTGCTGGATG AGGTGGAGTC TGCAAATCAC TGCTCCTTGT TTGAGCAGAA GACAGTCAAG
    GGATGGTGGC CGTGTGTAGG GCAGGAGGGG GACGAGAAGA TTCTGGCTGG AAAAGTGGAA
    ATTACTTTAG AGATTGTAAA TGAACAGGAA CATGATGAGA GACCGGCCGG GATTGGTCGA
    GATGAGCCAA ATATGAACCC AAGACTTGAA GACCCAAAGC GCCCAGAAAC CTCGTTCCTC
    TGGTTCACTT CCCCGTACAA GACCCTGAAG TACATCCTGT GGAGGAGATA CAAGTGGCTC
    ATCCTTGTCT TCTTCCTTCT ATTCATCCTA CTGCTCTTCC TCGGAATCTT CCTGTATTCC
    TTCCCGAACT ACGCTGCTAT GAAGCTGGTG AAGCCGTTCA GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751125.1
    CDS1273..7557
    Translation

    Target ORF information:

    RefSeq Version XM_031895265.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis dysferlin (LOC100488564), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031895265.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGCGCT GTGTGGTCCA AAGGGCCAGC AACCTCCCAA ATGTGGAGAA AGGGGTCAGG 
    TACAGTGACC CAGTTACCAG TTTAACCTTC AGAGGGGTTA AGAAGAAAAC GAAAATCATA
    AAGAACAATC TGAATCCCAT ATGGAACGAG GGATTCGAGT GGGATTTAAA AGGATTGCCC
    TTGGACAACA GTTCGGAGCT TGTCGCGGTA GTGAAAGACC ATGAAACCAT GGGAAGGAAC
    AGGTTTCTGG GTGAGACCCG AGTCAGCCTA CGGGATTTAC TTACGTCCCC TAACCTGACG
    GCCACGTATA ATGCCCCACT CCTGGACAGC AAGAAGCAGG GCACGGGGGC GATATTAACC
    TTGCAGATAT CTTACTTTCC TCCACCGGGG GCAGCCCCAG TTGTACCCCC TCCTGCACCC
    AAGGAGCCCA CCCCATCGCT CAATGAGTTG GACACCATCA CTGATACGGC TGGTGAAGAG
    GACACGGAAG ACCAGGCTGG AACTGGCGAT GAAGCGGATA TTGTCTCTCT GGCCCCTGGA
    GGAGCAGAGC AACCCACTCT CCCCAAGAAA ACTGCCCCTG TCCCTGTGCA GGGATTAAAG
    AGAAAGAAGA GCAACTCCAA AAAACACCTC TCCAACAAGC CTCAGGACTT CCAGGTTCGT
    GTTCGAGTGA TAGAAGGGAG GCAGATCCCA GGGATTAACA TCAAACCTGT TGTCAAAGTC
    ACAGTTGCTG GGCAGACCAA GAGAACCCGG ATACGCAAAG GGAACAACCC ATATTTCGAT
    GAGACATTTT TCTTCAATTT TTTTGAGTCC CCTTCTGAAC TCTTTGATGA ACCCGTGTTC
    CTGACTGTTC TCGATTCCCG CTCCCTCCGA ACAGACTCAG TGATTGGTGA ATTCCGGATG
    GACGTCGGAA TGATTTATGG AGAATCAAAA CATGCCTTCC TCAGGAAGTG GCTTCTCCTC
    TCCGACCCAG ACGATTTCTC CGCGGGGGCC AAGGGGTACC TGAAGGTCAG CCTCTATGTA
    CTTGGGCCTG GAGATGAGAG CCCGGTAGAG AAAAAGGAGG TCTCGGAAGA CAAGGAGGAC
    ATTGAGGGGA ACTTGCTGCG ACCGGCCGGG GTGTCCCTGC GAGGAGCCCA CCTTACTCTG
    AAGATTTACA GGGCGGAAGA TCTGCCGCAG ATGGACGATG CTGTGCTGGA CAATGTCAAA
    CAGATCTTTG GCTTTGACAG CAACAAGAAA AACTTGGTGG ATCCCTTTGT GGAAGTCAGT
    TTTGCGGGGA AGATGCTCCT CAGTAAGATC ATGGAGAAGA ACGCCAACCC GCAGTGGAGT
    CAGTCCATCA CTCTTCCCAT CAGGTTCCCC TCTATGTGTG AAAAAATGCG CATCCGCCTG
    TTGGACTGGG ACCGCCTCAC CCACAACGAT ATCATCGGCA CCACCTACCT GAGCATGTCC
    AAAATCTCAG CTCCTGGCGG CGAGATTGAA GATGAGACAC CGCGTAAGGC CTTGCCGGTT
    TCTAACTTTG ATGACACTTT GGGATTTCTG CCAACCTTCG GGCCTTGCTA TGTGAACCTT
    TATGGCAGCC CTCGGGAGTT CACTGGGTTT CCAGACCCTT ATGAAGATCT GAACTTGGGG
    AAGGGAGAAG GAGTGGCTTA CCGTGGTCGG GTCCTTCTGG AGTTGGAGAC AAAGCTGGTG
    GAGCAGGCTG AGCAGAAGGT GGAAGACATT CCTTCAGATG ATATTTCACG GGTTGAGAAA
    TATCTCCGCA GACGGAAGTA CTGCCTCTTT GCCGCTTTCT ATTCAGCCAC CATGTTGCAG
    GACGTCGATG ACGCCATTCA GTTTGAGGTC AGCATTGGAA ATTATGGAAA CAAGTTCGAC
    AACACCTGCC TCCCCCTGGC CTCCACCACG CAGTACAGCC GGGCTGTGTT CGACGGTTGC
    CAGTACTATT ATCTCCCCTG GGGTAACGTA AAGCCCGTTG TTGTTCTCTC ATCTCACTGG
    GAAGACATCG GCCACAGAAC TGACACTCAG AATATTCTAC AATACGCCAT CGATAATCTG
    GCAGCGAATC TGGACTGGGT CCACCTTGCT GTGAAAGCTA AGAGCTCCCC CTACGACTTG
    GACGCTTTGG TCGCGCGTTT GTTTGATGAC ATCATCACCG ACTGCAGCCA CGCCCTGCCA
    GATGTCAAGT CTATGCAAGG GACCACCCAC CTGGACCAGT ACTTGTACAG ATTCCGCAAG
    ACCAACCTTG CCCAGATTGT CCAGGCGGCT CTGAAACTCA AGCACGATGA CTGTGTGGAG
    CAGGAGAATG TGATGGAGCA GGCAGAGGAC TGGCTAATGC GACTAAAGGC TATGGCTGAG
    GAGCCGCAGA ACAGCCTGCC AGACATAGTG ATTTGGATGT TACAAGGGGA GAAGCGTGTG
    GCTTATTATA GAATGCCGGC CCGCGATGTC CTTTTCTCCA GGAATGGCTC CAAGTGTTGC
    GGCAAGAACT GCGGGAAGCT CCAGACAATA TTTCTCAAAT TTCCTCAGGA AGGAACCCAA
    GGAGCGAAGA TGCCAGCTCA GGTTCGAGTC CAGCTGTGGT TTGGGCTCGC TGTGGATCAG
    AAGGAATTCA ATCATTATGC TCAGGGGAAA TTGTCTGTTT TTGCTGAAGC TTATGAAAAC
    CAAACAAAGC TGGCTCTCGT GGGCAACTGG GGAACAACTG GCCTTACGTA CCCCAAATAC
    TCAGACGTGA CCGGGAAAAT CAAACTTCCA AAGGAGAGTT TCAAGGCTTC CACTGGATGG
    AGCTGGGCCG GAGACTGGTT TATCAGCCCG GAGAAAACGT TGCTGTATGA TACGGACGCG
    GGTCACATGA GCTTCGTGGA AGAGGTTTTC GAGAACCAGG CCAGGCTGCC CGGTGGGCAA
    TGGATCCATA TGACGGAGGC CTACTCAGAT GTGAATGGGG AGAAGGTTTT GCCAAAAGAT
    GAGATCGAAT GCCCCCCTGG CTGGAAATGG GAGGATGATG AATGGTCCAC GGACACCAAC
    AGGGCTGTTG ATGAGCAAGG ATGGGAGTAC GGCATTACTA TCCCCCCTGA CCGGAAGCCT
    AAATCCTGGG TTCCAGCAGA GAAGATGTAT CACACCAACC GCAGGAGGCG CTGGGTCAGA
    CTAAGACGAA GGGACCCCAA GCAGATTGAG ATCCTAAAAA AGCACAAGCC GGACGAGTCG
    GAGAGCGAGG GATGGGAATA CGCCTCTCTC TTTGGCTGGA AGTTTCACCT GAAGTACCGT
    AAGACTGACA GTTTCCGCAG GCGCAGGTGG AGGCGCCGTA TGGAACCCCT GGAACGCACC
    GGGGCGGCAG CTGTTTTCGC TTTGGAAGGT TCCCTGGGTG GAATTACAGA TGATAAAAGC
    GATGATGGAA AAAACACGTC CACAATGAGC TTCGGGGTCA ACCGGCCGAC GATATCCTGC
    ATATTTGACT ATGGGAACCG TTACCATTTG CGCTGCTACA TATACCAAGC GAGGAACCTG
    GTGCCCATGG ACCAAGACAG CTTTTCTGAT CCATACGCCA TAGTCTCTTT CCTCCATCAA
    AGTCAAAAGA CCTTGGTGAT GAAGAGCACC TTGAACCCAA CATGGGACCA GACCCTTATT
    TTTCATGAAA TTGAAGTTTT TGGAGACCCT GAAACCATAA AAGAATCCCC TCCCAACATA
    GTGGTGGAAA TCTATGACCA CGATACTTAC GGTGCAGATG AATACATGGG AAGGTGCATT
    TGCAAGCCAA GCTTTGAGCG CTCCCCCCGG TTATCTTGGC ACCCGGTGAT CATGGGCACG
    AAGAACGTCG GAGAGCTGCT CGCTGCCTTC GAGCTCATTC CCAGGGAAAA GCCGGCTGTT
    CATCTTATCC CAGGACTGGA GGGAGAAATC TCAGGAGTTC TGAGCGAGTC ATCAGACGCC
    GACCTTCCCT ACCCACCTCC ACAGAGGGAG GCCAATGTGT ACATGGTGCC CCAGGGAATC
    AAACCCGTTC TGCAGCGCAC CGCTATAGAG ATCCTGGCCT GGGGGCTGAG GAACCTAAAG
    AGCTACCAGC TGGCCAGCGT GACCTCCCCG AGTCTGATAG TGGAGTGCGG CGATGAACTG
    ATCCAGTCGG GGGTCATTAA GAACGTCAAG AAGAACCCCA ACTTTGATGT GTGCCTGCTC
    CTCATGGAAG TGAGGTTGCC CAGAGAAGAT CTATATTGTC CACCCATCAT TGTGAAGGTC
    ATCGATAACC GTCAGTTTGG AAGAAAACCA GTCGTCGGCC AATGCACCAT CAAGTCTTTG
    GAAGAGTTCC ATTGTGACCC CAACTCAGAA GATCTAGAGG TAGAAGATGG ACTGTCAGAC
    AATGTGAGTG TTACACCCAG GGACGAGGTG CTTATTGACA TTGATGATAA GGAACCTCTT
    ATCCCGACTC AGCTTGCCGA CGGTATGACC AGCTCAGCCA TTATTAACTT ATCATCTTCT
    CGGCCCAGTC TCCATGAAGA AGAGTTCATT GATTGGTGGA GTAAGTTTTA TGCTTCCATT
    GGGGAAAAAG AGAAATGTGG AACATACCTA GAGAAAGGCT TTGATACCTT GCAGGTACTG
    GACACAGAGC TAGAGAACAT AGAGGATTAC GAAGAACTCT CAGACTTTTG CCGAACCTTC
    AAACTGTACA GAGGAAAAAC ACAAGATGAG ACAGAAGATC CCTCTGTGGT TGGAGAATTT
    AAGGGATCCT TTAAAATCTA TCCTCTTCCT GATGACCCAT CAGTCCCCAG GCCAGCACCC
    CAGTTCCGCC AACTCCCTGC CAAAGGTCTT CAAGAGTGCC TCGTGCGTGT CTATATTGTT
    CGTGCTTTTG GTTTGCAGCC GAAAGACCCC AACGGCAAGT GTGATCCCTA CGTGAAGATT
    TCCATTGGAA AGAAGTCATT AAATGATCAG GAACATTATA TCCCTTGCAC CTTGGAGCCT
    GTTTTTGGAA AAATGTTTGA GCTGACCTGC ACCCTCCCTC TGGAGAAAGA CCTGAAGGTC
    ACACTCTATG ATTATGACAT GATTTCAAAA GATGAGAAGA TTGGAGAGAC GGTGATAGAC
    CTGGAGAACC GTTTCCTCTC GAAATATGGA GCAAGATGTG GATTGCCTCA ATCCTACTGC
    ATATCTGGGC CAAACCAGTG GCGGGACCAA CGGAAACCAT CGGAACTTCT CAGAATCTTT
    GCGCAGCAGC GGAACTTCAA ACCCCCTAAC TTCAAAAACA ACAGCATAAT GTTCCGGAAC
    CATGAGTACC ATGTCTCTGA GCTGGAAGAC AGCAAGTTCC CTAACCCGCA CCAGGGCCCG
    CCGGAGGAAC GGCTCGCTCT ACACGTACTG AGAAGGCAAG GCCTGGTGTG TGAGCATGTG
    GAGACCAGGG CCCTGTACAG CCCTTTGCAG CCGGATATAG AACAGGGAAA GCTGCAGATG
    TGGGTTGACT TGTTCCCCAA GTCCCTCGGT TCTCCCGGCC CTCCCTTTAA TATAACCCCA
    CGGAAAGCCA AACGGTATTA CTTGCGCTGT ATCATCTGGA ACACCAAAGA TGTGATTCTG
    GATGATGTGA GCATCACAGG AGAGAAGATG AGTGATATCT ATGTCAAGAG CTGGCTGATT
    GGCCATGAAG AAAATAAACA GAAGACAGAT GTTCACTACA GGTCTATGGG AGGTGAAGGG
    AATTTTAACT GGCGGCTTGT GTTCCCTTTC GACTATCTTC CCGCGGAGCA GGTCTGCTCA
    GTGTCCAGAA AGGAGCATTT CTGGAGCCTC GACAAGACTG AGAATAAAAT CCCTCCAAAC
    CTCATTATAC AGATATGGGA CAACGATAAG TTCTCCTTCG ATGACTATTT AGGTTCCGTG
    CAGCTGGACC TCAACCGGAT GCCACGACCT GCCAAAACGG CGGAGAAGTG CAGGCTGGAC
    CTGCTGGATG AGGTGGAGTC TGCAAATCAC TGCTCCTTGT TTGAGCAGAA GACAGTCAAG
    GGATGGTGGC CGTGTGTAGG GCAGGAGGGG GACGAGAAGA TTCTGGCTGG AAAAGTGGAA
    ATTACTTTAG AGATTGTAAA TGAACAGGAA CATGATGAGA GACCGGCCGG GATTGGTCGA
    GATGAGCCAA ATATGAACCC AAGACTTGAA GACCCAAAGC GCCCAGAAAC CTCGTTCCTC
    TGGTTCACTT CCCCGTACAA GACCCTGAAG TACATCCTGT GGAGGAGATA CAAGTGGCTC
    ATCCTTGTCT TCTTCCTTCT ATTCATCCTA CTGCTCTTCC TCGGAATCTT CCTGTATTCC
    TTCCCGAACT ACGCTGCTAT GAAGCTGGTG AAGCCGTTCA GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa49539
    Clone ID Related Accession (Same CDS sequence) XM_031895266.1
    Accession Version XM_031895266.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6285bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product dysferlin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGAGGG TCTTTATCCT TCATGCTGAA AATGTGCAAA CCCCAGACTC AGACATCAGC 
    GACTCCTACT GCTCTGTGGT GTTCCAAGGG GTTAAGAAGA AAACGAAAAT CATAAAGAAC
    AATCTGAATC CCATATGGAA CGAGGGATTC GAGTGGGATT TAAAAGGATT GCCCTTGGAC
    AACAGTTCGG AGCTTGTCGC GGTAGTGAAA GACCATGAAA CCATGGGAAG GAACAGGTTT
    CTGGGTGAGA CCCGAGTCAG CCTACGGGAT TTACTTACGT CCCCTAACCT GACGGCCACG
    TATAATGCCC CACTCCTGGA CAGCAAGAAG CAGGGCACGG GGGCGATATT AACCTTGCAG
    ATATCTTACT TTCCTCCACC GGGGGCAGCC CCAGTTGTAC CCCCTCCTGC ACCCAAGGAG
    CCCACCCCAT CGCTCAATGA GTTGGACACC ATCACTGATA CGGCTGGTGA AGAGGACACG
    GAAGACCAGG CTGGAACTGG CGATGAAGCG GATATTGTCT CTCTGGCCCC TGGAGGAGCA
    GAGCAACCCA CTCTCCCCAA GAAAACTGCC CCTGTCCCTG TGCAGGGATT AAAGAGAAAG
    AAGAGCAACT CCAAAAAACA CCTCTCCAAC AAGCCTCAGG ACTTCCAGGT TCGTGTTCGA
    GTGATAGAAG GGAGGCAGAT CCCAGGGATT AACATCAAAC CTGTTGTCAA AGTCACAGTT
    GCTGGGCAGA CCAAGAGAAC CCGGATACGC AAAGGGAACA ACCCATATTT CGATGAGACA
    TTTTTCTTCA ATTTTTTTGA GTCCCCTTCT GAACTCTTTG ATGAACCCGT GTTCCTGACT
    GTTCTCGATT CCCGCTCCCT CCGAACAGAC TCAGTGATTG GTGAATTCCG GATGGACGTC
    GGAATGATTT ATGGAGAATC AAAACATGCC TTCCTCAGGA AGTGGCTTCT CCTCTCCGAC
    CCAGACGATT TCTCCGCGGG GGCCAAGGGG TACCTGAAGG TCAGCCTCTA TGTACTTGGG
    CCTGGAGATG AGAGCCCGGT AGAGAAAAAG GAGGTCTCGG AAGACAAGGA GGACATTGAG
    GGGAACTTGC TGCGACCGGC CGGGGTGTCC CTGCGAGGAG CCCACCTTAC TCTGAAGATT
    TACAGGGCGG AAGATCTGCC GCAGATGGAC GATGCTGTGC TGGACAATGT CAAACAGATC
    TTTGGCTTTG ACAGCAACAA GAAAAACTTG GTGGATCCCT TTGTGGAAGT CAGTTTTGCG
    GGGAAGATGC TCCTCAGTAA GATCATGGAG AAGAACGCCA ACCCGCAGTG GAGTCAGTCC
    ATCACTCTTC CCATCAGGTT CCCCTCTATG TGTGAAAAAA TGCGCATCCG CCTGTTGGAC
    TGGGACCGCC TCACCCACAA CGATATCATC GGCACCACCT ACCTGAGCAT GTCCAAAATC
    TCAGCTCCTG GCGGCGAGAT TGAAGTTGAT GACACTTTGG GATTTCTGCC AACCTTCGGG
    CCTTGCTATG TGAACCTTTA TGGCAGCCCT CGGGAGTTCA CTGGGTTTCC AGACCCTTAT
    GAAGATCTGA ACTTGGGGAA GGGAGAAGGA GTGGCTTACC GTGGTCGGGT CCTTCTGGAG
    TTGGAGACAA AGCTGGTGGA GCAGGCTGAG CAGAAGGTGG AAGACATTCC TTCAGATGAT
    ATTTCACGGG TTGAGAAATA TCTCCGCAGA CGGAAGTACT GCCTCTTTGC CGCTTTCTAT
    TCAGCCACCA TGTTGCAGGA CGTCGATGAC GCCATTCAGT TTGAGGTCAG CATTGGAAAT
    TATGGAAACA AGTTCGACAA CACCTGCCTC CCCCTGGCCT CCACCACGCA GTACAGCCGG
    GCTGTGTTCG ACGGTTGCCA GTACTATTAT CTCCCCTGGG GTAACGTAAA GCCCGTTGTT
    GTTCTCTCAT CTCACTGGGA AGACATCGGC CACAGAACTG ACACTCAGAA TATTCTACAA
    TACGCCATCG ATAATCTGGC AGCGAATCTG GACTGGGTCC ACCTTGCTGT GAAAGCTAAG
    AGCTCCCCCT ACGACTTGGA CGCTTTGGTC GCGCGTTTGT TTGATGACAT CATCACCGAC
    TGCAGCCACG CCCTGCCAGA TGTCAAGTCT ATGCAAGGGA CCACCCACCT GGACCAGTAC
    TTGTACAGAT TCCGCAAGAC CAACCTTGCC CAGATTGTCC AGGCGGCTCT GAAACTCAAG
    CACGATGACT GTGTGGAGCA GGAGAATGTG ATGGAGCAGG CAGAGGACTG GCTAATGCGA
    CTAAAGGCTA TGGCTGAGGA GCCGCAGAAC AGCCTGCCAG ACATAGTGAT TTGGATGTTA
    CAAGGGGAGA AGCGTGTGGC TTATTATAGA ATGCCGGCCC GCGATGTCCT TTTCTCCAGG
    AATGGCTCCA AGTGTTGCGG CAAGAACTGC GGGAAGCTCC AGACAATATT TCTCAAATTT
    CCTCAGGAAG GAACCCAAGG AGCGAAGATG CCAGCTCAGG TTCGAGTCCA GCTGTGGTTT
    GGGCTCGCTG TGGATCAGAA GGAATTCAAT CATTATGCTC AGGGGAAATT GTCTGTTTTT
    GCTGAAGCTT ATGAAAACCA AACAAAGCTG GCTCTCGTGG GCAACTGGGG AACAACTGGC
    CTTACGTACC CCAAATACTC AGACGTGACC GGGAAAATCA AACTTCCAAA GGAGAGTTTC
    AAGGCTTCCA CTGGATGGAG CTGGGCCGGA GACTGGTTTA TCAGCCCGGA GAAAACGTTG
    CTGTATGATA CGGACGCGGG TCACATGAGC TTCGTGGAAG AGGTTTTCGA GAACCAGGCC
    AGGCTGCCCG GTGGGCAATG GATCCATATG ACGGAGGCCT ACTCAGATGT GAATGGGGAG
    AAGGTTTTGC CAAAAGATGA GATCGAATGC CCCCCTGGCT GGAAATGGGA GGATGATGAA
    TGGTCCACGG ACACCAACAG GGCTGTTGAT GAGCAAGGAT GGGAGTACGG CATTACTATC
    CCCCCTGACC GGAAGCCTAA ATCCTGGGTT CCAGCAGAGA AGATGTATCA CACCAACCGC
    AGGAGGCGCT GGGTCAGACT AAGACGAAGG GACCCCAAGC AGATTGAGAT CCTAAAAAAG
    CACAAGCCGG ACGAGTCGGA GAGCGAGGGA TGGGAATACG CCTCTCTCTT TGGCTGGAAG
    TTTCACCTGA AGTACCGTAA GACTGACAGT TTCCGCAGGC GCAGGTGGAG GCGCCGTATG
    GAACCCCTGG AACGCACCGG GGCGGCAGCT GTTTTCGCTT TGGAAGGTTC CCTGGGTGGA
    ATTACAGATG ATAAAAGCGA TGATGGAAAA AACACGTCCA CAATGAGCTT CGGGGTCAAC
    CGGCCGACGA TATCCTGCAT ATTTGACTAT GGGAACCGTT ACCATTTGCG CTGCTACATA
    TACCAAGCGA GGAACCTGGT GCCCATGGAC CAAGACAGCT TTTCTGATCC ATACGCCATA
    GTCTCTTTCC TCCATCAAAG TCAAAAGACC TTGGTGATGA AGAGCACCTT GAACCCAACA
    TGGGACCAGA CCCTTATTTT TCATGAAATT GAAGTTTTTG GAGACCCTGA AACCATAAAA
    GAATCCCCTC CCAACATAGT GGTGGAAATC TATGACCACG ATACTTACGG TGCAGATGAA
    TACATGGGAA GGTGCATTTG CAAGCCAAGC TTTGAGCGCT CCCCCCGGTT ATCTTGGCAC
    CCGGTGATCA TGGGCACGAA GAACGTCGGA GAGCTGCTCG CTGCCTTCGA GCTCATTCCC
    AGGGAAAAGC CGGCTGTTCA TCTTATCCCA GGACTGGAGG GAGAAATCTC AGGAGTTCTG
    AGCGAGCTGC TTATTCCTCG CTTCTTCACC GAGAAACTTC TTGACTGGTC ATCAGACGCC
    GACCTTCCCT ACCCACCTCC ACAGAGGGAG GCCAATGTGT ACATGGTGCC CCAGGGAATC
    AAACCCGTTC TGCAGCGCAC CGCTATAGAG ATCCTGGCCT GGGGGCTGAG GAACCTAAAG
    AGCTACCAGC TGGCCAGCGT GACCTCCCCG AGTCTGATAG TGGAGTGCGG CGATGAACTG
    ATCCAGTCGG GGGTCATTAA GAACGTCAAG AAGAACCCCA ACTTTGATGT GTGCCTGCTC
    CTCATGGAAG TGAGGTTGCC CAGAGAAGAT CTATATTGTC CACCCATCAT TGTGAAGGTC
    ATCGATAACC GTCAGTTTGG AAGAAAACCA GTCGTCGGCC AATGCACCAT CAAGTCTTTG
    GAAGAGTTCC ATTGTGACCC CAACTCAGAA GATCTAGAGG TAGAAGATGG ACTGTCAGAC
    AATGTGAGTG TTACACCCAG GGACGAGGTG CTTATTGACA TTGATGATAA GGAACCTCTT
    ATCCCGACTC AGCTTGCCGA CGGTATGACC AGCTCAGCCA TTATTAACTT ATCATCTTCT
    CGGCCCAGTC TCCATGAAGA AGAGTTCATT GATTGGTGGA GTAAGTTTTA TGCTTCCATT
    GGGGAAAAAG AGAAATGTGG AACATACCTA GAGAAAGGCT TTGATACCTT GCAGGTACTG
    GACACAGAGC TAGAGAACAT AGAGGATTAC GAAGAACTCT CAGACTTTTG CCGAACCTTC
    AAACTGTACA GAGGAAAAAC ACAAGATGAG ACAGAAGATC CCTCTGTGGT TGGAGAATTT
    AAGGGATCCT TTAAAATCTA TCCTCTTCCT GATGACCCAT CAGTCCCCAG GCCAGCACCC
    CAGTTCCGCC AACTCCCTGC CAAAGGTCTT CAAGAGTGCC TCGTGCGTGT CTATATTGTT
    CGTGCTTTTG GTTTGCAGCC GAAAGACCCC AACGGCAAGT GTGATCCCTA CGTGAAGATT
    TCCATTGGAA AGAAGTCATT AAATGATCAG GAACATTATA TCCCTTGCAC CTTGGAGCCT
    GTTTTTGGAA AAATGTTTGA GCTGACCTGC ACCCTCCCTC TGGAGAAAGA CCTGAAGGTC
    ACACTCTATG ATTATGACAT GATTTCAAAA GATGAGAAGA TTGGAGAGAC GGTGATAGAC
    CTGGAGAACC GTTTCCTCTC GAAATATGGA GCAAGATGTG GATTGCCTCA ATCCTACTGC
    ATATCTGGGC CAAACCAGTG GCGGGACCAA CGGAAACCAT CGGAACTTCT CAGAATCTTT
    GCGCAGCAGC GGAACTTCAA ACCCCCTAAC TTCAAAAACA ACAGCATAAT GTTCCGGAAC
    CATGAGTACC ATGTCTCTGA GCTGGAAGAC AGCAAGTTCC CTAACCCGCA CCAGGGCCCG
    CCGGAGGAAC GGCTCGCTCT ACACGTACTG AGAAGGCAAG GCCTGGTGTG TGAGCATGTG
    GAGACCAGGG CCCTGTACAG CCCTTTGCAG CCGGATATAG AACAGGGAAA GCTGCAGATG
    TGGGTTGACT TGTTCCCCAA GTCCCTCGGT TCTCCCGGCC CTCCCTTTAA TATAACCCCA
    CGGAAAGCCA AACGGTATTA CTTGCGCTGT ATCATCTGGA ACACCAAAGA TGTGATTCTG
    GATGATGTGA GCATCACAGG AGAGAAGATG AGTGATATCT ATGTCAAGAG CTGGCTGATT
    GGCCATGAAG AAAATAAACA GAAGACAGAT GTTCACTACA GGTCTATGGG AGGTGAAGGG
    AATTTTAACT GGCGGCTTGT GTTCCCTTTC GACTATCTTC CCGCGGAGCA GGTCTGCTCA
    GTGTCCAGAA AGGAGCATTT CTGGAGCCTC GACAAGACTG AGAATAAAAT CCCTCCAAAC
    CTCATTATAC AGATATGGGA CAACGATAAG TTCTCCTTCG ATGACTATTT AGGTTCCGTG
    CAGCTGGACC TCAACCGGAT GCCACGACCT GCCAAAACGG CGGAGAAGTG CAGGCTGGAC
    CTGCTGGATG AGGTGGAGTC TGCAAATCAC TGCTCCTTGT TTGAGCAGAA GACAGTCAAG
    GGATGGTGGC CGTGTGTAGG GCAGGAGGGG GACGAGAAGA TTCTGGCTGG AAAAGTGGAA
    ATTACTTTAG AGATTGTAAA TGAACAGGAA CATGATGAGA GACCGGCCGG GATTGGTCGA
    GATGAGCCAA ATATGAACCC AAGACTTGAA GACCCAAAGC GCCCAGAAAC CTCGTTCCTC
    TGGTTCACTT CCCCGTACAA GACCCTGAAG TACATCCTGT GGAGGAGATA CAAGTGGCTC
    ATCCTTGTCT TCTTCCTTCT ATTCATCCTA CTGCTCTTCC TCGGAATCTT CCTGTATTCC
    TTCCCGAACT ACGCTGCTAT GAAGCTGGTG AAGCCGTTCA GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751126.1
    CDS139..6423
    Translation

    Target ORF information:

    RefSeq Version XM_031895266.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis dysferlin (LOC100488564), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031895266.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGAGGG TCTTTATCCT TCATGCTGAA AATGTGCAAA CCCCAGACTC AGACATCAGC 
    GACTCCTACT GCTCTGTGGT GTTCCAAGGG GTTAAGAAGA AAACGAAAAT CATAAAGAAC
    AATCTGAATC CCATATGGAA CGAGGGATTC GAGTGGGATT TAAAAGGATT GCCCTTGGAC
    AACAGTTCGG AGCTTGTCGC GGTAGTGAAA GACCATGAAA CCATGGGAAG GAACAGGTTT
    CTGGGTGAGA CCCGAGTCAG CCTACGGGAT TTACTTACGT CCCCTAACCT GACGGCCACG
    TATAATGCCC CACTCCTGGA CAGCAAGAAG CAGGGCACGG GGGCGATATT AACCTTGCAG
    ATATCTTACT TTCCTCCACC GGGGGCAGCC CCAGTTGTAC CCCCTCCTGC ACCCAAGGAG
    CCCACCCCAT CGCTCAATGA GTTGGACACC ATCACTGATA CGGCTGGTGA AGAGGACACG
    GAAGACCAGG CTGGAACTGG CGATGAAGCG GATATTGTCT CTCTGGCCCC TGGAGGAGCA
    GAGCAACCCA CTCTCCCCAA GAAAACTGCC CCTGTCCCTG TGCAGGGATT AAAGAGAAAG
    AAGAGCAACT CCAAAAAACA CCTCTCCAAC AAGCCTCAGG ACTTCCAGGT TCGTGTTCGA
    GTGATAGAAG GGAGGCAGAT CCCAGGGATT AACATCAAAC CTGTTGTCAA AGTCACAGTT
    GCTGGGCAGA CCAAGAGAAC CCGGATACGC AAAGGGAACA ACCCATATTT CGATGAGACA
    TTTTTCTTCA ATTTTTTTGA GTCCCCTTCT GAACTCTTTG ATGAACCCGT GTTCCTGACT
    GTTCTCGATT CCCGCTCCCT CCGAACAGAC TCAGTGATTG GTGAATTCCG GATGGACGTC
    GGAATGATTT ATGGAGAATC AAAACATGCC TTCCTCAGGA AGTGGCTTCT CCTCTCCGAC
    CCAGACGATT TCTCCGCGGG GGCCAAGGGG TACCTGAAGG TCAGCCTCTA TGTACTTGGG
    CCTGGAGATG AGAGCCCGGT AGAGAAAAAG GAGGTCTCGG AAGACAAGGA GGACATTGAG
    GGGAACTTGC TGCGACCGGC CGGGGTGTCC CTGCGAGGAG CCCACCTTAC TCTGAAGATT
    TACAGGGCGG AAGATCTGCC GCAGATGGAC GATGCTGTGC TGGACAATGT CAAACAGATC
    TTTGGCTTTG ACAGCAACAA GAAAAACTTG GTGGATCCCT TTGTGGAAGT CAGTTTTGCG
    GGGAAGATGC TCCTCAGTAA GATCATGGAG AAGAACGCCA ACCCGCAGTG GAGTCAGTCC
    ATCACTCTTC CCATCAGGTT CCCCTCTATG TGTGAAAAAA TGCGCATCCG CCTGTTGGAC
    TGGGACCGCC TCACCCACAA CGATATCATC GGCACCACCT ACCTGAGCAT GTCCAAAATC
    TCAGCTCCTG GCGGCGAGAT TGAAGTTGAT GACACTTTGG GATTTCTGCC AACCTTCGGG
    CCTTGCTATG TGAACCTTTA TGGCAGCCCT CGGGAGTTCA CTGGGTTTCC AGACCCTTAT
    GAAGATCTGA ACTTGGGGAA GGGAGAAGGA GTGGCTTACC GTGGTCGGGT CCTTCTGGAG
    TTGGAGACAA AGCTGGTGGA GCAGGCTGAG CAGAAGGTGG AAGACATTCC TTCAGATGAT
    ATTTCACGGG TTGAGAAATA TCTCCGCAGA CGGAAGTACT GCCTCTTTGC CGCTTTCTAT
    TCAGCCACCA TGTTGCAGGA CGTCGATGAC GCCATTCAGT TTGAGGTCAG CATTGGAAAT
    TATGGAAACA AGTTCGACAA CACCTGCCTC CCCCTGGCCT CCACCACGCA GTACAGCCGG
    GCTGTGTTCG ACGGTTGCCA GTACTATTAT CTCCCCTGGG GTAACGTAAA GCCCGTTGTT
    GTTCTCTCAT CTCACTGGGA AGACATCGGC CACAGAACTG ACACTCAGAA TATTCTACAA
    TACGCCATCG ATAATCTGGC AGCGAATCTG GACTGGGTCC ACCTTGCTGT GAAAGCTAAG
    AGCTCCCCCT ACGACTTGGA CGCTTTGGTC GCGCGTTTGT TTGATGACAT CATCACCGAC
    TGCAGCCACG CCCTGCCAGA TGTCAAGTCT ATGCAAGGGA CCACCCACCT GGACCAGTAC
    TTGTACAGAT TCCGCAAGAC CAACCTTGCC CAGATTGTCC AGGCGGCTCT GAAACTCAAG
    CACGATGACT GTGTGGAGCA GGAGAATGTG ATGGAGCAGG CAGAGGACTG GCTAATGCGA
    CTAAAGGCTA TGGCTGAGGA GCCGCAGAAC AGCCTGCCAG ACATAGTGAT TTGGATGTTA
    CAAGGGGAGA AGCGTGTGGC TTATTATAGA ATGCCGGCCC GCGATGTCCT TTTCTCCAGG
    AATGGCTCCA AGTGTTGCGG CAAGAACTGC GGGAAGCTCC AGACAATATT TCTCAAATTT
    CCTCAGGAAG GAACCCAAGG AGCGAAGATG CCAGCTCAGG TTCGAGTCCA GCTGTGGTTT
    GGGCTCGCTG TGGATCAGAA GGAATTCAAT CATTATGCTC AGGGGAAATT GTCTGTTTTT
    GCTGAAGCTT ATGAAAACCA AACAAAGCTG GCTCTCGTGG GCAACTGGGG AACAACTGGC
    CTTACGTACC CCAAATACTC AGACGTGACC GGGAAAATCA AACTTCCAAA GGAGAGTTTC
    AAGGCTTCCA CTGGATGGAG CTGGGCCGGA GACTGGTTTA TCAGCCCGGA GAAAACGTTG
    CTGTATGATA CGGACGCGGG TCACATGAGC TTCGTGGAAG AGGTTTTCGA GAACCAGGCC
    AGGCTGCCCG GTGGGCAATG GATCCATATG ACGGAGGCCT ACTCAGATGT GAATGGGGAG
    AAGGTTTTGC CAAAAGATGA GATCGAATGC CCCCCTGGCT GGAAATGGGA GGATGATGAA
    TGGTCCACGG ACACCAACAG GGCTGTTGAT GAGCAAGGAT GGGAGTACGG CATTACTATC
    CCCCCTGACC GGAAGCCTAA ATCCTGGGTT CCAGCAGAGA AGATGTATCA CACCAACCGC
    AGGAGGCGCT GGGTCAGACT AAGACGAAGG GACCCCAAGC AGATTGAGAT CCTAAAAAAG
    CACAAGCCGG ACGAGTCGGA GAGCGAGGGA TGGGAATACG CCTCTCTCTT TGGCTGGAAG
    TTTCACCTGA AGTACCGTAA GACTGACAGT TTCCGCAGGC GCAGGTGGAG GCGCCGTATG
    GAACCCCTGG AACGCACCGG GGCGGCAGCT GTTTTCGCTT TGGAAGGTTC CCTGGGTGGA
    ATTACAGATG ATAAAAGCGA TGATGGAAAA AACACGTCCA CAATGAGCTT CGGGGTCAAC
    CGGCCGACGA TATCCTGCAT ATTTGACTAT GGGAACCGTT ACCATTTGCG CTGCTACATA
    TACCAAGCGA GGAACCTGGT GCCCATGGAC CAAGACAGCT TTTCTGATCC ATACGCCATA
    GTCTCTTTCC TCCATCAAAG TCAAAAGACC TTGGTGATGA AGAGCACCTT GAACCCAACA
    TGGGACCAGA CCCTTATTTT TCATGAAATT GAAGTTTTTG GAGACCCTGA AACCATAAAA
    GAATCCCCTC CCAACATAGT GGTGGAAATC TATGACCACG ATACTTACGG TGCAGATGAA
    TACATGGGAA GGTGCATTTG CAAGCCAAGC TTTGAGCGCT CCCCCCGGTT ATCTTGGCAC
    CCGGTGATCA TGGGCACGAA GAACGTCGGA GAGCTGCTCG CTGCCTTCGA GCTCATTCCC
    AGGGAAAAGC CGGCTGTTCA TCTTATCCCA GGACTGGAGG GAGAAATCTC AGGAGTTCTG
    AGCGAGCTGC TTATTCCTCG CTTCTTCACC GAGAAACTTC TTGACTGGTC ATCAGACGCC
    GACCTTCCCT ACCCACCTCC ACAGAGGGAG GCCAATGTGT ACATGGTGCC CCAGGGAATC
    AAACCCGTTC TGCAGCGCAC CGCTATAGAG ATCCTGGCCT GGGGGCTGAG GAACCTAAAG
    AGCTACCAGC TGGCCAGCGT GACCTCCCCG AGTCTGATAG TGGAGTGCGG CGATGAACTG
    ATCCAGTCGG GGGTCATTAA GAACGTCAAG AAGAACCCCA ACTTTGATGT GTGCCTGCTC
    CTCATGGAAG TGAGGTTGCC CAGAGAAGAT CTATATTGTC CACCCATCAT TGTGAAGGTC
    ATCGATAACC GTCAGTTTGG AAGAAAACCA GTCGTCGGCC AATGCACCAT CAAGTCTTTG
    GAAGAGTTCC ATTGTGACCC CAACTCAGAA GATCTAGAGG TAGAAGATGG ACTGTCAGAC
    AATGTGAGTG TTACACCCAG GGACGAGGTG CTTATTGACA TTGATGATAA GGAACCTCTT
    ATCCCGACTC AGCTTGCCGA CGGTATGACC AGCTCAGCCA TTATTAACTT ATCATCTTCT
    CGGCCCAGTC TCCATGAAGA AGAGTTCATT GATTGGTGGA GTAAGTTTTA TGCTTCCATT
    GGGGAAAAAG AGAAATGTGG AACATACCTA GAGAAAGGCT TTGATACCTT GCAGGTACTG
    GACACAGAGC TAGAGAACAT AGAGGATTAC GAAGAACTCT CAGACTTTTG CCGAACCTTC
    AAACTGTACA GAGGAAAAAC ACAAGATGAG ACAGAAGATC CCTCTGTGGT TGGAGAATTT
    AAGGGATCCT TTAAAATCTA TCCTCTTCCT GATGACCCAT CAGTCCCCAG GCCAGCACCC
    CAGTTCCGCC AACTCCCTGC CAAAGGTCTT CAAGAGTGCC TCGTGCGTGT CTATATTGTT
    CGTGCTTTTG GTTTGCAGCC GAAAGACCCC AACGGCAAGT GTGATCCCTA CGTGAAGATT
    TCCATTGGAA AGAAGTCATT AAATGATCAG GAACATTATA TCCCTTGCAC CTTGGAGCCT
    GTTTTTGGAA AAATGTTTGA GCTGACCTGC ACCCTCCCTC TGGAGAAAGA CCTGAAGGTC
    ACACTCTATG ATTATGACAT GATTTCAAAA GATGAGAAGA TTGGAGAGAC GGTGATAGAC
    CTGGAGAACC GTTTCCTCTC GAAATATGGA GCAAGATGTG GATTGCCTCA ATCCTACTGC
    ATATCTGGGC CAAACCAGTG GCGGGACCAA CGGAAACCAT CGGAACTTCT CAGAATCTTT
    GCGCAGCAGC GGAACTTCAA ACCCCCTAAC TTCAAAAACA ACAGCATAAT GTTCCGGAAC
    CATGAGTACC ATGTCTCTGA GCTGGAAGAC AGCAAGTTCC CTAACCCGCA CCAGGGCCCG
    CCGGAGGAAC GGCTCGCTCT ACACGTACTG AGAAGGCAAG GCCTGGTGTG TGAGCATGTG
    GAGACCAGGG CCCTGTACAG CCCTTTGCAG CCGGATATAG AACAGGGAAA GCTGCAGATG
    TGGGTTGACT TGTTCCCCAA GTCCCTCGGT TCTCCCGGCC CTCCCTTTAA TATAACCCCA
    CGGAAAGCCA AACGGTATTA CTTGCGCTGT ATCATCTGGA ACACCAAAGA TGTGATTCTG
    GATGATGTGA GCATCACAGG AGAGAAGATG AGTGATATCT ATGTCAAGAG CTGGCTGATT
    GGCCATGAAG AAAATAAACA GAAGACAGAT GTTCACTACA GGTCTATGGG AGGTGAAGGG
    AATTTTAACT GGCGGCTTGT GTTCCCTTTC GACTATCTTC CCGCGGAGCA GGTCTGCTCA
    GTGTCCAGAA AGGAGCATTT CTGGAGCCTC GACAAGACTG AGAATAAAAT CCCTCCAAAC
    CTCATTATAC AGATATGGGA CAACGATAAG TTCTCCTTCG ATGACTATTT AGGTTCCGTG
    CAGCTGGACC TCAACCGGAT GCCACGACCT GCCAAAACGG CGGAGAAGTG CAGGCTGGAC
    CTGCTGGATG AGGTGGAGTC TGCAAATCAC TGCTCCTTGT TTGAGCAGAA GACAGTCAAG
    GGATGGTGGC CGTGTGTAGG GCAGGAGGGG GACGAGAAGA TTCTGGCTGG AAAAGTGGAA
    ATTACTTTAG AGATTGTAAA TGAACAGGAA CATGATGAGA GACCGGCCGG GATTGGTCGA
    GATGAGCCAA ATATGAACCC AAGACTTGAA GACCCAAAGC GCCCAGAAAC CTCGTTCCTC
    TGGTTCACTT CCCCGTACAA GACCCTGAAG TACATCCTGT GGAGGAGATA CAAGTGGCTC
    ATCCTTGTCT TCTTCCTTCT ATTCATCCTA CTGCTCTTCC TCGGAATCTT CCTGTATTCC
    TTCCCGAACT ACGCTGCTAT GAAGCTGGTG AAGCCGTTCA GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa49540
    Clone ID Related Accession (Same CDS sequence) XM_031895267.1
    Accession Version XM_031895267.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6264bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product dysferlin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGCGCT GTGTGGTCCA AAGGGCCAGC AACCTCCCAA ATGTGGAGAA AGGGGTCAGG 
    TACAGTGACC CAGTTACCAG TTTAACCTTC AGAGGGGTTA AGAAGAAAAC GAAAATCATA
    AAGAACAATC TGAATCCCAT ATGGAACGAG GGATTCGAGT GGGATTTAAA AGGATTGCCC
    TTGGACAACA GTTCGGAGCT TGTCGCGGTA GTGAAAGACC ATGAAACCAT GGGAAGGAAC
    AGGTTTCTGG GTGAGACCCG AGTCAGCCTA CGGGATTTAC TTACGTCCCC TAACCTGACG
    GCCACGTATA ATGCCCCACT CCTGGACAGC AAGAAGCAGG GCACGGGGGC GATATTAACC
    TTGCAGATAT CTTACTTTCC TCCACCGGGG GCAGCCCCAG TTGTACCCCC TCCTGCACCC
    AAGGAGCCCA CCCCATCGCT CAATGAGTTG GACACCATCA CTGATACGGC TGGTGAAGAG
    GACACGGAAG ACCAGGCTGG AACTGGCGAT GAAGCGGATA TTGTCTCTCT GGCCCCTGGA
    GGAGCAGAGC AACCCACTCT CCCCAAGAAA ACTGCCCCTG TCCCTGTGCA GGGATTAAAG
    AGAAAGAAGA GCAACTCCAA AAAACACCTC TCCAACAAGC CTCAGGACTT CCAGGTTCGT
    GTTCGAGTGA TAGAAGGGAG GCAGATCCCA GGGATTAACA TCAAACCTGT TGTCAAAGTC
    ACAGTTGCTG GGCAGACCAA GAGAACCCGG ATACGCAAAG GGAACAACCC ATATTTCGAT
    GAGACATTTT TCTTCAATTT TTTTGAGTCC CCTTCTGAAC TCTTTGATGA ACCCGTGTTC
    CTGACTGTTC TCGATTCCCG CTCCCTCCGA ACAGACTCAG TGATTGGTGA ATTCCGGATG
    GACGTCGGAA TGATTTATGG AGAATCAAAA CATGCCTTCC TCAGGAAGTG GCTTCTCCTC
    TCCGACCCAG ACGATTTCTC CGCGGGGGCC AAGGGGTACC TGAAGGTCAG CCTCTATGTA
    CTTGGGCCTG GAGATGAGAG CCCGGTAGAG AAAAAGGAGG TCTCGGAAGA CAAGGAGGAC
    ATTGAGGGGA ACTTGCTGCG ACCGGCCGGG GTGTCCCTGC GAGGAGCCCA CCTTACTCTG
    AAGATTTACA GGGCGGAAGA TCTGCCGCAG ATGGACGATG CTGTGCTGGA CAATGTCAAA
    CAGATCTTTG GCTTTGACAG CAACAAGAAA AACTTGGTGG ATCCCTTTGT GGAAGTCAGT
    TTTGCGGGGA AGATGCTCCT CAGTAAGATC ATGGAGAAGA ACGCCAACCC GCAGTGGAGT
    CAGTCCATCA CTCTTCCCAT CAGGTTCCCC TCTATGTGTG AAAAAATGCG CATCCGCCTG
    TTGGACTGGG ACCGCCTCAC CCACAACGAT ATCATCGGCA CCACCTACCT GAGCATGTCC
    AAAATCTCAG CTCCTGGCGG CGAGATTGAA GATGAGACAC CGCGTAAGGC CTTGCCGGTT
    TCTAACTTTG ATGACACTTT GGGATTTCTG CCAACCTTCG GGCCTTGCTA TGTGAACCTT
    TATGGCAGCC CTCGGGAGTT CACTGGGTTT CCAGACCCTT ATGAAGATCT GAACTTGGGG
    AAGGGAGAAG GAGTGGCTTA CCGTGGTCGG GTCCTTCTGG AGTTGGAGAC AAAGCTGGTG
    GAGCAGGCTG AGCAGAAGGT GGAAGACATT CCTTCAGATG ATATTTCACG GGTTGAGAAA
    TATCTCCGCA GACGGAAGTA CTGCCTCTTT GCCGCTTTCT ATTCAGCCAC CATGTTGCAG
    GACGTCGATG ACGCCATTCA GTTTGAGGTC AGCATTGGAA ATTATGGAAA CAAGTTCGAC
    AACACCTGCC TCCCCCTGGC CTCCACCACG CAGTACAGCC GGGCTGTGTT CGACGGTTGC
    CAGTACTATT ATCTCCCCTG GGGTAACGTA AAGCCCGTTG TTGTTCTCTC ATCTCACTGG
    GAAGACATCG GCCACAGAAC TGACACTCAG AATATTCTAC AATACGCCAT CGATAATCTG
    GCAGCGAATC TGGACTGGGT CCACCTTGCT GTGAAAGCTA AGAGCTCCCC CTACGACTTG
    GACGCTTTGG TCGCGCGTTT GTTTGATGAC ATCATCACCG ACTGCAGCCA CGCCCTGCCA
    GATGTCAAGT CTATGCAAGG GACCACCCAC CTGGACCAGT ACTTGTACAG ATTCCGCAAG
    ACCAACCTTG CCCAGATTGT CCAGGCGGCT CTGAAACTCA AGCACGATGA CTGTGTGGAG
    CAGGAGAATG TGATGGAGCA GGCAGAGGAC TGGCTAATGC GACTAAAGGC TATGGCTGAG
    GAGCCGCAGA ACAGCCTGCC AGACATAGTG ATTTGGATGT TACAAGGGGA GAAGCGTGTG
    GCTTATTATA GAATGCCGGC CCGCGATGTC CTTTTCTCCA GGAATGGCTC CAAGTGTTGC
    GGCAAGAACT GCGGGAAGCT CCAGACAATA TTTCTCAAAT TTCCTCAGGA AGGAACCCAA
    GGAGCGAAGA TGCCAGCTCA GGTTCGAGTC CAGCTGTGGT TTGGGCTCGC TGTGGATCAG
    AAGGAATTCA ATCATTATGC TCAGGGGAAA TTGTCTGTTT TTGCTGAAGC TTATGAAAAC
    CAAACAAAGC TGGCTCTCGT GGGCAACTGG GGAACAACTG GCCTTACGTA CCCCAAATAC
    TCAGACGTGA CCGGGAAAAT CAAACTTCCA AAGGAGAGTT TCAAGGCTTC CACTGGATGG
    AGCTGGGCCG GAGACTGGTT TATCAGCCCG GAGAAAACGT TGCTGTATGA TACGGACGCG
    GGTCACATGA GCTTCGTGGA AGAGGTTTTC GAGAACCAGG CCAGGCTGCC CGGTGGGCAA
    TGGATCCATA TGACGGAGGC CTACTCAGAT GTGAATGGGG AGAAGGTTTT GCCAAAAGAT
    GAGATCGAAT GCCCCCCTGG CTGGAAATGG GAGGATGATG AATGGTCCAC GGACACCAAC
    AGGGCTGTTG ATGAGCAAGG ATGGGAGTAC GGCATTACTA TCCCCCCTGA CCGGAAGCCT
    AAATCCTGGG TTCCAGCAGA GAAGATGTAT CACACCAACC GCAGGAGGCG CTGGGTCAGA
    CTAAGACGAA GGGACCCCAA GCAGATTGAG ATCCTAAAAA AGCACAAGCC GGACGAGTCG
    GAGAGCGAGG GATGGGAATA CGCCTCTCTC TTTGGCTGGA AGTTTCACCT GAAGTACCGT
    AAGACTGACA GTTTCCGCAG GCGCAGGTGG AGGCGCCGTA TGGAACCCCT GGAACGCACC
    GGGGCGGCAG CTGTTTTCGC TTTGGAAGGT TCCCTGGGTG GAATTACAGA TGATAAAAGC
    GATGATGGAA AAAACACGTC CACAATGAGC TTCGGGGTCA ACCGGCCGAC GATATCCTGC
    ATATTTGACT ATGGGAACCG TTACCATTTG CGCTGCTACA TATACCAAGC GAGGAACCTG
    GTGCCCATGG ACCAAGACAG CTTTTCTGAT CCATACGCCA TAGTCTCTTT CCTCCATCAA
    AGTCAAAAGA CCTTGGTGAT GAAGAGCACC TTGAACCCAA CATGGGACCA GACCCTTATT
    TTTCATGAAA TTGAAGTTTT TGGAGACCCT GAAACCATAA AAGAATCCCC TCCCAACATA
    GTGGTGGAAA TCTATGACCA CGATACTTAC GGTGCAGATG AATACATGGG AAGGTGCATT
    TGCAAGCCAA GCTTTGAGCG CTCCCCCCGG TTATCTTGGC ACCCGGTGAT CATGGGCACG
    AAGAACGTCG GAGAGCTGCT CGCTGCCTTC GAGCTCATTC CCAGGGAAAA GCCGGCTGTT
    CATCTTATCC CAGGACTGGA GGGAGAAATC TCAGGAGTTC TGAGCGAGCT GCTTATTCCT
    CGCTTCTTCA CCGAGAAACT TCTTGACTGG TCATCAGACG CCGACCTTCC CTACCCACCT
    CCACAGAGGG AGGCCAATGT GTACATGGTG CCCCAGGGAA TCAAACCCGT TCTGCAGCGC
    ACCGCTATAG AGATCCTGGC CTGGGGGCTG AGGAACCTAA AGAGCTACCA GCTGGCCAGC
    GTGACCTCCC CGAGTCTGAT AGTGGAGTGC GGCGATGAAC TGATCCAGTC GGGGGTCATT
    AAGAACGTCA AGAAGAACCC CAACTTTGAT GTGTGCCTGC TCCTCATGGA AGTGAGGTTG
    CCCAGAGAAG ATCTATATTG TCCACCCATC ATTGTGAAGG TCATCGATAA CCGTCAGTTT
    GGAAGAAAAC CAGTCGTCGG CCAATGCACC ATCAAGTCTT TGGAAGAGTT CCATTGTGAC
    CCCAACTCAG AAGATCTAGA GGTAGAAGAT GGACTGTCAG ACAATGTGAG TGTTACACCC
    AGGGACGAGG TGCTTATTGA CATTGATGAT AAGGAACCTC TTATCCCGAC TCAGGAAGAA
    GAGTTCATTG ATTGGTGGAG TAAGTTTTAT GCTTCCATTG GGGAAAAAGA GAAATGTGGA
    ACATACCTAG AGAAAGGCTT TGATACCTTG CAGGTACTGG ACACAGAGCT AGAGAACATA
    GAGGATTACG AAGAACTCTC AGACTTTTGC CGAACCTTCA AACTGTACAG AGGAAAAACA
    CAAGATGAGA CAGAAGATCC CTCTGTGGTT GGAGAATTTA AGGGATCCTT TAAAATCTAT
    CCTCTTCCTG ATGACCCATC AGTCCCCAGG CCAGCACCCC AGTTCCGCCA ACTCCCTGCC
    AAAGGTCTTC AAGAGTGCCT CGTGCGTGTC TATATTGTTC GTGCTTTTGG TTTGCAGCCG
    AAAGACCCCA ACGGCAAGTG TGATCCCTAC GTGAAGATTT CCATTGGAAA GAAGTCATTA
    AATGATCAGG AACATTATAT CCCTTGCACC TTGGAGCCTG TTTTTGGAAA AATGTTTGAG
    CTGACCTGCA CCCTCCCTCT GGAGAAAGAC CTGAAGGTCA CACTCTATGA TTATGACATG
    ATTTCAAAAG ATGAGAAGAT TGGAGAGACG GTGATAGACC TGGAGAACCG TTTCCTCTCG
    AAATATGGAG CAAGATGTGG ATTGCCTCAA TCCTACTGCA TATCTGGGCC AAACCAGTGG
    CGGGACCAAC GGAAACCATC GGAACTTCTC AGAATCTTTG CGCAGCAGCG GAACTTCAAA
    CCCCCTAACT TCAAAAACAA CAGCATAATG TTCCGGAACC ATGAGTACCA TGTCTCTGAG
    CTGGAAGACA GCAAGTTCCC TAACCCGCAC CAGGGCCCGC CGGAGGAACG GCTCGCTCTA
    CACGTACTGA GAAGGCAAGG CCTGGTGTGT GAGCATGTGG AGACCAGGGC CCTGTACAGC
    CCTTTGCAGC CGGATATAGA ACAGGGAAAG CTGCAGATGT GGGTTGACTT GTTCCCCAAG
    TCCCTCGGTT CTCCCGGCCC TCCCTTTAAT ATAACCCCAC GGAAAGCCAA ACGGTATTAC
    TTGCGCTGTA TCATCTGGAA CACCAAAGAT GTGATTCTGG ATGATGTGAG CATCACAGGA
    GAGAAGATGA GTGATATCTA TGTCAAGAGC TGGCTGATTG GCCATGAAGA AAATAAACAG
    AAGACAGATG TTCACTACAG GTCTATGGGA GGTGAAGGGA ATTTTAACTG GCGGCTTGTG
    TTCCCTTTCG ACTATCTTCC CGCGGAGCAG GTCTGCTCAG TGTCCAGAAA GGAGCATTTC
    TGGAGCCTCG ACAAGACTGA GAATAAAATC CCTCCAAACC TCATTATACA GATATGGGAC
    AACGATAAGT TCTCCTTCGA TGACTATTTA GGTTCCGTGC AGCTGGACCT CAACCGGATG
    CCACGACCTG CCAAAACGGC GGAGAAGTGC AGGCTGGACC TGCTGGATGA GGTGGAGTCT
    GCAAATCACT GCTCCTTGTT TGAGCAGAAG ACAGTCAAGG GATGGTGGCC GTGTGTAGGG
    CAGGAGGGGG ACGAGAAGAT TCTGGCTGGA AAAGTGGAAA TTACTTTAGA GATTGTAAAT
    GAACAGGAAC ATGATGAGAG ACCGGCCGGG ATTGGTCGAG ATGAGCCAAA TATGAACCCA
    AGACTTGAAG ACCCAAAGCG CCCAGAAACC TCGTTCCTCT GGTTCACTTC CCCGTACAAG
    ACCCTGAAGT ACATCCTGTG GAGGAGATAC AAGTGGCTCA TCCTTGTCTT CTTCCTTCTA
    TTCATCCTAC TGCTCTTCCT CGGAATCTTC CTGTATTCCT TCCCGAACTA CGCTGCTATG
    AAGCTGGTGA AGCCGTTCAG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751127.1
    CDS1273..7536
    Translation

    Target ORF information:

    RefSeq Version XM_031895267.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis dysferlin (LOC100488564), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031895267.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGCGCT GTGTGGTCCA AAGGGCCAGC AACCTCCCAA ATGTGGAGAA AGGGGTCAGG 
    TACAGTGACC CAGTTACCAG TTTAACCTTC AGAGGGGTTA AGAAGAAAAC GAAAATCATA
    AAGAACAATC TGAATCCCAT ATGGAACGAG GGATTCGAGT GGGATTTAAA AGGATTGCCC
    TTGGACAACA GTTCGGAGCT TGTCGCGGTA GTGAAAGACC ATGAAACCAT GGGAAGGAAC
    AGGTTTCTGG GTGAGACCCG AGTCAGCCTA CGGGATTTAC TTACGTCCCC TAACCTGACG
    GCCACGTATA ATGCCCCACT CCTGGACAGC AAGAAGCAGG GCACGGGGGC GATATTAACC
    TTGCAGATAT CTTACTTTCC TCCACCGGGG GCAGCCCCAG TTGTACCCCC TCCTGCACCC
    AAGGAGCCCA CCCCATCGCT CAATGAGTTG GACACCATCA CTGATACGGC TGGTGAAGAG
    GACACGGAAG ACCAGGCTGG AACTGGCGAT GAAGCGGATA TTGTCTCTCT GGCCCCTGGA
    GGAGCAGAGC AACCCACTCT CCCCAAGAAA ACTGCCCCTG TCCCTGTGCA GGGATTAAAG
    AGAAAGAAGA GCAACTCCAA AAAACACCTC TCCAACAAGC CTCAGGACTT CCAGGTTCGT
    GTTCGAGTGA TAGAAGGGAG GCAGATCCCA GGGATTAACA TCAAACCTGT TGTCAAAGTC
    ACAGTTGCTG GGCAGACCAA GAGAACCCGG ATACGCAAAG GGAACAACCC ATATTTCGAT
    GAGACATTTT TCTTCAATTT TTTTGAGTCC CCTTCTGAAC TCTTTGATGA ACCCGTGTTC
    CTGACTGTTC TCGATTCCCG CTCCCTCCGA ACAGACTCAG TGATTGGTGA ATTCCGGATG
    GACGTCGGAA TGATTTATGG AGAATCAAAA CATGCCTTCC TCAGGAAGTG GCTTCTCCTC
    TCCGACCCAG ACGATTTCTC CGCGGGGGCC AAGGGGTACC TGAAGGTCAG CCTCTATGTA
    CTTGGGCCTG GAGATGAGAG CCCGGTAGAG AAAAAGGAGG TCTCGGAAGA CAAGGAGGAC
    ATTGAGGGGA ACTTGCTGCG ACCGGCCGGG GTGTCCCTGC GAGGAGCCCA CCTTACTCTG
    AAGATTTACA GGGCGGAAGA TCTGCCGCAG ATGGACGATG CTGTGCTGGA CAATGTCAAA
    CAGATCTTTG GCTTTGACAG CAACAAGAAA AACTTGGTGG ATCCCTTTGT GGAAGTCAGT
    TTTGCGGGGA AGATGCTCCT CAGTAAGATC ATGGAGAAGA ACGCCAACCC GCAGTGGAGT
    CAGTCCATCA CTCTTCCCAT CAGGTTCCCC TCTATGTGTG AAAAAATGCG CATCCGCCTG
    TTGGACTGGG ACCGCCTCAC CCACAACGAT ATCATCGGCA CCACCTACCT GAGCATGTCC
    AAAATCTCAG CTCCTGGCGG CGAGATTGAA GATGAGACAC CGCGTAAGGC CTTGCCGGTT
    TCTAACTTTG ATGACACTTT GGGATTTCTG CCAACCTTCG GGCCTTGCTA TGTGAACCTT
    TATGGCAGCC CTCGGGAGTT CACTGGGTTT CCAGACCCTT ATGAAGATCT GAACTTGGGG
    AAGGGAGAAG GAGTGGCTTA CCGTGGTCGG GTCCTTCTGG AGTTGGAGAC AAAGCTGGTG
    GAGCAGGCTG AGCAGAAGGT GGAAGACATT CCTTCAGATG ATATTTCACG GGTTGAGAAA
    TATCTCCGCA GACGGAAGTA CTGCCTCTTT GCCGCTTTCT ATTCAGCCAC CATGTTGCAG
    GACGTCGATG ACGCCATTCA GTTTGAGGTC AGCATTGGAA ATTATGGAAA CAAGTTCGAC
    AACACCTGCC TCCCCCTGGC CTCCACCACG CAGTACAGCC GGGCTGTGTT CGACGGTTGC
    CAGTACTATT ATCTCCCCTG GGGTAACGTA AAGCCCGTTG TTGTTCTCTC ATCTCACTGG
    GAAGACATCG GCCACAGAAC TGACACTCAG AATATTCTAC AATACGCCAT CGATAATCTG
    GCAGCGAATC TGGACTGGGT CCACCTTGCT GTGAAAGCTA AGAGCTCCCC CTACGACTTG
    GACGCTTTGG TCGCGCGTTT GTTTGATGAC ATCATCACCG ACTGCAGCCA CGCCCTGCCA
    GATGTCAAGT CTATGCAAGG GACCACCCAC CTGGACCAGT ACTTGTACAG ATTCCGCAAG
    ACCAACCTTG CCCAGATTGT CCAGGCGGCT CTGAAACTCA AGCACGATGA CTGTGTGGAG
    CAGGAGAATG TGATGGAGCA GGCAGAGGAC TGGCTAATGC GACTAAAGGC TATGGCTGAG
    GAGCCGCAGA ACAGCCTGCC AGACATAGTG ATTTGGATGT TACAAGGGGA GAAGCGTGTG
    GCTTATTATA GAATGCCGGC CCGCGATGTC CTTTTCTCCA GGAATGGCTC CAAGTGTTGC
    GGCAAGAACT GCGGGAAGCT CCAGACAATA TTTCTCAAAT TTCCTCAGGA AGGAACCCAA
    GGAGCGAAGA TGCCAGCTCA GGTTCGAGTC CAGCTGTGGT TTGGGCTCGC TGTGGATCAG
    AAGGAATTCA ATCATTATGC TCAGGGGAAA TTGTCTGTTT TTGCTGAAGC TTATGAAAAC
    CAAACAAAGC TGGCTCTCGT GGGCAACTGG GGAACAACTG GCCTTACGTA CCCCAAATAC
    TCAGACGTGA CCGGGAAAAT CAAACTTCCA AAGGAGAGTT TCAAGGCTTC CACTGGATGG
    AGCTGGGCCG GAGACTGGTT TATCAGCCCG GAGAAAACGT TGCTGTATGA TACGGACGCG
    GGTCACATGA GCTTCGTGGA AGAGGTTTTC GAGAACCAGG CCAGGCTGCC CGGTGGGCAA
    TGGATCCATA TGACGGAGGC CTACTCAGAT GTGAATGGGG AGAAGGTTTT GCCAAAAGAT
    GAGATCGAAT GCCCCCCTGG CTGGAAATGG GAGGATGATG AATGGTCCAC GGACACCAAC
    AGGGCTGTTG ATGAGCAAGG ATGGGAGTAC GGCATTACTA TCCCCCCTGA CCGGAAGCCT
    AAATCCTGGG TTCCAGCAGA GAAGATGTAT CACACCAACC GCAGGAGGCG CTGGGTCAGA
    CTAAGACGAA GGGACCCCAA GCAGATTGAG ATCCTAAAAA AGCACAAGCC GGACGAGTCG
    GAGAGCGAGG GATGGGAATA CGCCTCTCTC TTTGGCTGGA AGTTTCACCT GAAGTACCGT
    AAGACTGACA GTTTCCGCAG GCGCAGGTGG AGGCGCCGTA TGGAACCCCT GGAACGCACC
    GGGGCGGCAG CTGTTTTCGC TTTGGAAGGT TCCCTGGGTG GAATTACAGA TGATAAAAGC
    GATGATGGAA AAAACACGTC CACAATGAGC TTCGGGGTCA ACCGGCCGAC GATATCCTGC
    ATATTTGACT ATGGGAACCG TTACCATTTG CGCTGCTACA TATACCAAGC GAGGAACCTG
    GTGCCCATGG ACCAAGACAG CTTTTCTGAT CCATACGCCA TAGTCTCTTT CCTCCATCAA
    AGTCAAAAGA CCTTGGTGAT GAAGAGCACC TTGAACCCAA CATGGGACCA GACCCTTATT
    TTTCATGAAA TTGAAGTTTT TGGAGACCCT GAAACCATAA AAGAATCCCC TCCCAACATA
    GTGGTGGAAA TCTATGACCA CGATACTTAC GGTGCAGATG AATACATGGG AAGGTGCATT
    TGCAAGCCAA GCTTTGAGCG CTCCCCCCGG TTATCTTGGC ACCCGGTGAT CATGGGCACG
    AAGAACGTCG GAGAGCTGCT CGCTGCCTTC GAGCTCATTC CCAGGGAAAA GCCGGCTGTT
    CATCTTATCC CAGGACTGGA GGGAGAAATC TCAGGAGTTC TGAGCGAGCT GCTTATTCCT
    CGCTTCTTCA CCGAGAAACT TCTTGACTGG TCATCAGACG CCGACCTTCC CTACCCACCT
    CCACAGAGGG AGGCCAATGT GTACATGGTG CCCCAGGGAA TCAAACCCGT TCTGCAGCGC
    ACCGCTATAG AGATCCTGGC CTGGGGGCTG AGGAACCTAA AGAGCTACCA GCTGGCCAGC
    GTGACCTCCC CGAGTCTGAT AGTGGAGTGC GGCGATGAAC TGATCCAGTC GGGGGTCATT
    AAGAACGTCA AGAAGAACCC CAACTTTGAT GTGTGCCTGC TCCTCATGGA AGTGAGGTTG
    CCCAGAGAAG ATCTATATTG TCCACCCATC ATTGTGAAGG TCATCGATAA CCGTCAGTTT
    GGAAGAAAAC CAGTCGTCGG CCAATGCACC ATCAAGTCTT TGGAAGAGTT CCATTGTGAC
    CCCAACTCAG AAGATCTAGA GGTAGAAGAT GGACTGTCAG ACAATGTGAG TGTTACACCC
    AGGGACGAGG TGCTTATTGA CATTGATGAT AAGGAACCTC TTATCCCGAC TCAGGAAGAA
    GAGTTCATTG ATTGGTGGAG TAAGTTTTAT GCTTCCATTG GGGAAAAAGA GAAATGTGGA
    ACATACCTAG AGAAAGGCTT TGATACCTTG CAGGTACTGG ACACAGAGCT AGAGAACATA
    GAGGATTACG AAGAACTCTC AGACTTTTGC CGAACCTTCA AACTGTACAG AGGAAAAACA
    CAAGATGAGA CAGAAGATCC CTCTGTGGTT GGAGAATTTA AGGGATCCTT TAAAATCTAT
    CCTCTTCCTG ATGACCCATC AGTCCCCAGG CCAGCACCCC AGTTCCGCCA ACTCCCTGCC
    AAAGGTCTTC AAGAGTGCCT CGTGCGTGTC TATATTGTTC GTGCTTTTGG TTTGCAGCCG
    AAAGACCCCA ACGGCAAGTG TGATCCCTAC GTGAAGATTT CCATTGGAAA GAAGTCATTA
    AATGATCAGG AACATTATAT CCCTTGCACC TTGGAGCCTG TTTTTGGAAA AATGTTTGAG
    CTGACCTGCA CCCTCCCTCT GGAGAAAGAC CTGAAGGTCA CACTCTATGA TTATGACATG
    ATTTCAAAAG ATGAGAAGAT TGGAGAGACG GTGATAGACC TGGAGAACCG TTTCCTCTCG
    AAATATGGAG CAAGATGTGG ATTGCCTCAA TCCTACTGCA TATCTGGGCC AAACCAGTGG
    CGGGACCAAC GGAAACCATC GGAACTTCTC AGAATCTTTG CGCAGCAGCG GAACTTCAAA
    CCCCCTAACT TCAAAAACAA CAGCATAATG TTCCGGAACC ATGAGTACCA TGTCTCTGAG
    CTGGAAGACA GCAAGTTCCC TAACCCGCAC CAGGGCCCGC CGGAGGAACG GCTCGCTCTA
    CACGTACTGA GAAGGCAAGG CCTGGTGTGT GAGCATGTGG AGACCAGGGC CCTGTACAGC
    CCTTTGCAGC CGGATATAGA ACAGGGAAAG CTGCAGATGT GGGTTGACTT GTTCCCCAAG
    TCCCTCGGTT CTCCCGGCCC TCCCTTTAAT ATAACCCCAC GGAAAGCCAA ACGGTATTAC
    TTGCGCTGTA TCATCTGGAA CACCAAAGAT GTGATTCTGG ATGATGTGAG CATCACAGGA
    GAGAAGATGA GTGATATCTA TGTCAAGAGC TGGCTGATTG GCCATGAAGA AAATAAACAG
    AAGACAGATG TTCACTACAG GTCTATGGGA GGTGAAGGGA ATTTTAACTG GCGGCTTGTG
    TTCCCTTTCG ACTATCTTCC CGCGGAGCAG GTCTGCTCAG TGTCCAGAAA GGAGCATTTC
    TGGAGCCTCG ACAAGACTGA GAATAAAATC CCTCCAAACC TCATTATACA GATATGGGAC
    AACGATAAGT TCTCCTTCGA TGACTATTTA GGTTCCGTGC AGCTGGACCT CAACCGGATG
    CCACGACCTG CCAAAACGGC GGAGAAGTGC AGGCTGGACC TGCTGGATGA GGTGGAGTCT
    GCAAATCACT GCTCCTTGTT TGAGCAGAAG ACAGTCAAGG GATGGTGGCC GTGTGTAGGG
    CAGGAGGGGG ACGAGAAGAT TCTGGCTGGA AAAGTGGAAA TTACTTTAGA GATTGTAAAT
    GAACAGGAAC ATGATGAGAG ACCGGCCGGG ATTGGTCGAG ATGAGCCAAA TATGAACCCA
    AGACTTGAAG ACCCAAAGCG CCCAGAAACC TCGTTCCTCT GGTTCACTTC CCCGTACAAG
    ACCCTGAAGT ACATCCTGTG GAGGAGATAC AAGTGGCTCA TCCTTGTCTT CTTCCTTCTA
    TTCATCCTAC TGCTCTTCCT CGGAATCTTC CTGTATTCCT TCCCGAACTA CGCTGCTATG
    AAGCTGGTGA AGCCGTTCAG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa49541
    Clone ID Related Accession (Same CDS sequence) XM_031895268.1
    Accession Version XM_031895268.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6228bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product dysferlin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTGCGCT GTGTGGTCCA AAGGGCCAGC AACCTCCCAA ATGTGGAGAA AGGGGTCAGG 
    TACAGTGACC CAGTTACCAG TTTAACCTTC AGAGGGGTTA AGAAGAAAAC GAAAATCATA
    AAGAACAATC TGAATCCCAT ATGGAACGAG GGATTCGAGT GGGATTTAAA AGGATTGCCC
    TTGGACAACA GTTCGGAGCT TGTCGCGGTA GTGAAAGACC ATGAAACCAT GGGAAGGAAC
    AGGTTTCTGG GTGAGACCCG AGTCAGCCTA CGGGATTTAC TTACGTCCCC TAACCTGACG
    GCCACGTATA ATGCCCCACT CCTGGACAGC AAGAAGCAGG GCACGGGGGC GATATTAACC
    TTGCAGATAT CTTACTTTCC TCCACCGGGG GCAGCCCCAG TTGTACCCCC TCCTGCACCC
    AAGGAGCCCA CCCCATCGCT CAATGAGTTG GACACCATCA CTGATACGGC TGGTGAAGAG
    GACACGGAAG ACCAGGCTGG AACTGGCGAT GAAGCGGATA TTGTCTCTCT GGCCCCTGGA
    GGAGCAGAGC AACCCACTCT CCCCAAGAAA ACTGCCCCTG TCCCTGTGCA GGGATTAAAG
    AGAAAGAAGA GCAACTCCAA AAAACACCTC TCCAACAAGC CTCAGGACTT CCAGGTTCGT
    GTTCGAGTGA TAGAAGGGAG GCAGATCCCA GGGATTAACA TCAAACCTGT TGTCAAAGTC
    ACAGTTGCTG GGCAGACCAA GAGAACCCGG ATACGCAAAG GGAACAACCC ATATTTCGAT
    GAGACATTTT TCTTCAATTT TTTTGAGTCC CCTTCTGAAC TCTTTGATGA ACCCGTGTTC
    CTGACTGTTC TCGATTCCCG CTCCCTCCGA ACAGACTCAG TGATTGGTGA ATTCCGGATG
    GACGTCGGAA TGATTTATGG AGAATCAAAA CATGCCTTCC TCAGGAAGTG GCTTCTCCTC
    TCCGACCCAG ACGATTTCTC CGCGGGGGCC AAGGGGTACC TGAAGGTCAG CCTCTATGTA
    CTTGGGCCTG GAGATGAGAG CCCGGTAGAG AAAAAGGAGG TCTCGGAAGA CAAGGAGGAC
    ATTGAGGGGA ACTTGCTGCG ACCGGCCGGG GTGTCCCTGC GAGGAGCCCA CCTTACTCTG
    AAGATTTACA GGGCGGAAGA TCTGCCGCAG ATGGACGATG CTGTGCTGGA CAATGTCAAA
    CAGATCTTTG GCTTTGACAG CAACAAGAAA AACTTGGTGG ATCCCTTTGT GGAAGTCAGT
    TTTGCGGGGA AGATGCTCCT CAGTAAGATC ATGGAGAAGA ACGCCAACCC GCAGTGGAGT
    CAGTCCATCA CTCTTCCCAT CAGGTTCCCC TCTATGTGTG AAAAAATGCG CATCCGCCTG
    TTGGACTGGG ACCGCCTCAC CCACAACGAT ATCATCGGCA CCACCTACCT GAGCATGTCC
    AAAATCTCAG CTCCTGGCGG CGAGATTGAA GTTGATGACA CTTTGGGATT TCTGCCAACC
    TTCGGGCCTT GCTATGTGAA CCTTTATGGC AGCCCTCGGG AGTTCACTGG GTTTCCAGAC
    CCTTATGAAG ATCTGAACTT GGGGAAGGGA GAAGGAGTGG CTTACCGTGG TCGGGTCCTT
    CTGGAGTTGG AGACAAAGCT GGTGGAGCAG GCTGAGCAGA AGGTGGAAGA CATTCCTTCA
    GATGATATTT CACGGGTTGA GAAATATCTC CGCAGACGGA AGTACTGCCT CTTTGCCGCT
    TTCTATTCAG CCACCATGTT GCAGGACGTC GATGACGCCA TTCAGTTTGA GGTCAGCATT
    GGAAATTATG GAAACAAGTT CGACAACACC TGCCTCCCCC TGGCCTCCAC CACGCAGTAC
    AGCCGGGCTG TGTTCGACGG TTGCCAGTAC TATTATCTCC CCTGGGGTAA CGTAAAGCCC
    GTTGTTGTTC TCTCATCTCA CTGGGAAGAC ATCGGCCACA GAACTGACAC TCAGAATATT
    CTACAATACG CCATCGATAA TCTGGCAGCG AATCTGGACT GGGTCCACCT TGCTGTGAAA
    GCTAAGAGCT CCCCCTACGA CTTGGACGCT TTGGTCGCGC GTTTGTTTGA TGACATCATC
    ACCGACTGCA GCCACGCCCT GCCAGATGTC AAGTCTATGC AAGGGACCAC CCACCTGGAC
    CAGTACTTGT ACAGATTCCG CAAGACCAAC CTTGCCCAGA TTGTCCAGGC GGCTCTGAAA
    CTCAAGCACG ATGACTGTGT GGAGCAGGAG AATGTGATGG AGCAGGCAGA GGACTGGCTA
    ATGCGACTAA AGGCTATGGC TGAGGAGCCG CAGAACAGCC TGCCAGACAT AGTGATTTGG
    ATGTTACAAG GGGAGAAGCG TGTGGCTTAT TATAGAATGC CGGCCCGCGA TGTCCTTTTC
    TCCAGGAATG GCTCCAAGTG TTGCGGCAAG AACTGCGGGA AGCTCCAGAC AATATTTCTC
    AAATTTCCTC AGGAAGGAAC CCAAGGAGCG AAGATGCCAG CTCAGGTTCG AGTCCAGCTG
    TGGTTTGGGC TCGCTGTGGA TCAGAAGGAA TTCAATCATT ATGCTCAGGG GAAATTGTCT
    GTTTTTGCTG AAGCTTATGA AAACCAAACA AAGCTGGCTC TCGTGGGCAA CTGGGGAACA
    ACTGGCCTTA CGTACCCCAA ATACTCAGAC GTGACCGGGA AAATCAAACT TCCAAAGGAG
    AGTTTCAAGG CTTCCACTGG ATGGAGCTGG GCCGGAGACT GGTTTATCAG CCCGGAGAAA
    ACGTTGCTGT ATGATACGGA CGCGGGTCAC ATGAGCTTCG TGGAAGAGGT TTTCGAGAAC
    CAGGCCAGGC TGCCCGGTGG GCAATGGATC CATATGACGG AGGCCTACTC AGATGTGAAT
    GGGGAGAAGG TTTTGCCAAA AGATGAGATC GAATGCCCCC CTGGCTGGAA ATGGGAGGAT
    GATGAATGGT CCACGGACAC CAACAGGGCT GTTGATGAGC AAGGATGGGA GTACGGCATT
    ACTATCCCCC CTGACCGGAA GCCTAAATCC TGGGTTCCAG CAGAGAAGAT GTATCACACC
    AACCGCAGGA GGCGCTGGGT CAGACTAAGA CGAAGGGACC CCAAGCAGAT TGAGATCCTA
    AAAAAGCACA AGCCGGACGA GTCGGAGAGC GAGGGATGGG AATACGCCTC TCTCTTTGGC
    TGGAAGTTTC ACCTGAAGTA CCGTAAGACT GACAGTTTCC GCAGGCGCAG GTGGAGGCGC
    CGTATGGAAC CCCTGGAACG CACCGGGGCG GCAGCTGTTT TCGCTTTGGA AGGTTCCCTG
    GGTGGAATTA CAGATGATAA AAGCGATGAT GGAAAAAACA CGTCCACAAT GAGCTTCGGG
    GTCAACCGGC CGACGATATC CTGCATATTT GACTATGGGA ACCGTTACCA TTTGCGCTGC
    TACATATACC AAGCGAGGAA CCTGGTGCCC ATGGACCAAG ACAGCTTTTC TGATCCATAC
    GCCATAGTCT CTTTCCTCCA TCAAAGTCAA AAGACCTTGG TGATGAAGAG CACCTTGAAC
    CCAACATGGG ACCAGACCCT TATTTTTCAT GAAATTGAAG TTTTTGGAGA CCCTGAAACC
    ATAAAAGAAT CCCCTCCCAA CATAGTGGTG GAAATCTATG ACCACGATAC TTACGGTGCA
    GATGAATACA TGGGAAGGTG CATTTGCAAG CCAAGCTTTG AGCGCTCCCC CCGGTTATCT
    TGGCACCCGG TGATCATGGG CACGAAGAAC GTCGGAGAGC TGCTCGCTGC CTTCGAGCTC
    ATTCCCAGGG AAAAGCCGGC TGTTCATCTT ATCCCAGGAC TGGAGGGAGA AATCTCAGGA
    GTTCTGAGCG AGCTGCTTAT TCCTCGCTTC TTCACCGAGA AACTTCTTGA CTGGTCATCA
    GACGCCGACC TTCCCTACCC ACCTCCACAG AGGGAGGCCA ATGTGTACAT GGTGCCCCAG
    GGAATCAAAC CCGTTCTGCA GCGCACCGCT ATAGAGATCC TGGCCTGGGG GCTGAGGAAC
    CTAAAGAGCT ACCAGCTGGC CAGCGTGACC TCCCCGAGTC TGATAGTGGA GTGCGGCGAT
    GAACTGATCC AGTCGGGGGT CATTAAGAAC GTCAAGAAGA ACCCCAACTT TGATGTGTGC
    CTGCTCCTCA TGGAAGTGAG GTTGCCCAGA GAAGATCTAT ATTGTCCACC CATCATTGTG
    AAGGTCATCG ATAACCGTCA GTTTGGAAGA AAACCAGTCG TCGGCCAATG CACCATCAAG
    TCTTTGGAAG AGTTCCATTG TGACCCCAAC TCAGAAGATC TAGAGGTAGA AGATGGACTG
    TCAGACAATG TGAGTGTTAC ACCCAGGGAC GAGGTGCTTA TTGACATTGA TGATAAGGAA
    CCTCTTATCC CGACTCAGGA AGAAGAGTTC ATTGATTGGT GGAGTAAGTT TTATGCTTCC
    ATTGGGGAAA AAGAGAAATG TGGAACATAC CTAGAGAAAG GCTTTGATAC CTTGCAGGTA
    CTGGACACAG AGCTAGAGAA CATAGAGGAT TACGAAGAAC TCTCAGACTT TTGCCGAACC
    TTCAAACTGT ACAGAGGAAA AACACAAGAT GAGACAGAAG ATCCCTCTGT GGTTGGAGAA
    TTTAAGGGAT CCTTTAAAAT CTATCCTCTT CCTGATGACC CATCAGTCCC CAGGCCAGCA
    CCCCAGTTCC GCCAACTCCC TGCCAAAGGT CTTCAAGAGT GCCTCGTGCG TGTCTATATT
    GTTCGTGCTT TTGGTTTGCA GCCGAAAGAC CCCAACGGCA AGTGTGATCC CTACGTGAAG
    ATTTCCATTG GAAAGAAGTC ATTAAATGAT CAGGAACATT ATATCCCTTG CACCTTGGAG
    CCTGTTTTTG GAAAAATGTT TGAGCTGACC TGCACCCTCC CTCTGGAGAA AGACCTGAAG
    GTCACACTCT ATGATTATGA CATGATTTCA AAAGATGAGA AGATTGGAGA GACGGTGATA
    GACCTGGAGA ACCGTTTCCT CTCGAAATAT GGAGCAAGAT GTGGATTGCC TCAATCCTAC
    TGCATATCTG GGCCAAACCA GTGGCGGGAC CAACGGAAAC CATCGGAACT TCTCAGAATC
    TTTGCGCAGC AGCGGAACTT CAAACCCCCT AACTTCAAAA ACAACAGCAT AATGTTCCGG
    AACCATGAGT ACCATGTCTC TGAGCTGGAA GACAGCAAGT TCCCTAACCC GCACCAGGGC
    CCGCCGGAGG AACGGCTCGC TCTACACGTA CTGAGAAGGC AAGGCCTGGT GTGTGAGCAT
    GTGGAGACCA GGGCCCTGTA CAGCCCTTTG CAGCCGGATA TAGAACAGGG AAAGCTGCAG
    ATGTGGGTTG ACTTGTTCCC CAAGTCCCTC GGTTCTCCCG GCCCTCCCTT TAATATAACC
    CCACGGAAAG CCAAACGGTA TTACTTGCGC TGTATCATCT GGAACACCAA AGATGTGATT
    CTGGATGATG TGAGCATCAC AGGAGAGAAG ATGAGTGATA TCTATGTCAA GAGCTGGCTG
    ATTGGCCATG AAGAAAATAA ACAGAAGACA GATGTTCACT ACAGGTCTAT GGGAGGTGAA
    GGGAATTTTA ACTGGCGGCT TGTGTTCCCT TTCGACTATC TTCCCGCGGA GCAGGTCTGC
    TCAGTGTCCA GAAAGGAGCA TTTCTGGAGC CTCGACAAGA CTGAGAATAA AATCCCTCCA
    AACCTCATTA TACAGATATG GGACAACGAT AAGTTCTCCT TCGATGACTA TTTAGGTTCC
    GTGCAGCTGG ACCTCAACCG GATGCCACGA CCTGCCAAAA CGGCGGAGAA GTGCAGGCTG
    GACCTGCTGG ATGAGGTGGA GTCTGCAAAT CACTGCTCCT TGTTTGAGCA GAAGACAGTC
    AAGGGATGGT GGCCGTGTGT AGGGCAGGAG GGGGACGAGA AGATTCTGGC TGGAAAAGTG
    GAAATTACTT TAGAGATTGT AAATGAACAG GAACATGATG AGAGACCGGC CGGGATTGGT
    CGAGATGAGC CAAATATGAA CCCAAGACTT GAAGACCCAA AGCGCCCAGA AACCTCGTTC
    CTCTGGTTCA CTTCCCCGTA CAAGACCCTG AAGTACATCC TGTGGAGGAG ATACAAGTGG
    CTCATCCTTG TCTTCTTCCT TCTATTCATC CTACTGCTCT TCCTCGGAAT CTTCCTGTAT
    TCCTTCCCGA ACTACGCTGC TATGAAGCTG GTGAAGCCGT TCAGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751128.1
    CDS1273..7500
    Translation

    Target ORF information:

    RefSeq Version XM_031895268.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis dysferlin (LOC100488564), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031895268.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTGCGCT GTGTGGTCCA AAGGGCCAGC AACCTCCCAA ATGTGGAGAA AGGGGTCAGG 
    TACAGTGACC CAGTTACCAG TTTAACCTTC AGAGGGGTTA AGAAGAAAAC GAAAATCATA
    AAGAACAATC TGAATCCCAT ATGGAACGAG GGATTCGAGT GGGATTTAAA AGGATTGCCC
    TTGGACAACA GTTCGGAGCT TGTCGCGGTA GTGAAAGACC ATGAAACCAT GGGAAGGAAC
    AGGTTTCTGG GTGAGACCCG AGTCAGCCTA CGGGATTTAC TTACGTCCCC TAACCTGACG
    GCCACGTATA ATGCCCCACT CCTGGACAGC AAGAAGCAGG GCACGGGGGC GATATTAACC
    TTGCAGATAT CTTACTTTCC TCCACCGGGG GCAGCCCCAG TTGTACCCCC TCCTGCACCC
    AAGGAGCCCA CCCCATCGCT CAATGAGTTG GACACCATCA CTGATACGGC TGGTGAAGAG
    GACACGGAAG ACCAGGCTGG AACTGGCGAT GAAGCGGATA TTGTCTCTCT GGCCCCTGGA
    GGAGCAGAGC AACCCACTCT CCCCAAGAAA ACTGCCCCTG TCCCTGTGCA GGGATTAAAG
    AGAAAGAAGA GCAACTCCAA AAAACACCTC TCCAACAAGC CTCAGGACTT CCAGGTTCGT
    GTTCGAGTGA TAGAAGGGAG GCAGATCCCA GGGATTAACA TCAAACCTGT TGTCAAAGTC
    ACAGTTGCTG GGCAGACCAA GAGAACCCGG ATACGCAAAG GGAACAACCC ATATTTCGAT
    GAGACATTTT TCTTCAATTT TTTTGAGTCC CCTTCTGAAC TCTTTGATGA ACCCGTGTTC
    CTGACTGTTC TCGATTCCCG CTCCCTCCGA ACAGACTCAG TGATTGGTGA ATTCCGGATG
    GACGTCGGAA TGATTTATGG AGAATCAAAA CATGCCTTCC TCAGGAAGTG GCTTCTCCTC
    TCCGACCCAG ACGATTTCTC CGCGGGGGCC AAGGGGTACC TGAAGGTCAG CCTCTATGTA
    CTTGGGCCTG GAGATGAGAG CCCGGTAGAG AAAAAGGAGG TCTCGGAAGA CAAGGAGGAC
    ATTGAGGGGA ACTTGCTGCG ACCGGCCGGG GTGTCCCTGC GAGGAGCCCA CCTTACTCTG
    AAGATTTACA GGGCGGAAGA TCTGCCGCAG ATGGACGATG CTGTGCTGGA CAATGTCAAA
    CAGATCTTTG GCTTTGACAG CAACAAGAAA AACTTGGTGG ATCCCTTTGT GGAAGTCAGT
    TTTGCGGGGA AGATGCTCCT CAGTAAGATC ATGGAGAAGA ACGCCAACCC GCAGTGGAGT
    CAGTCCATCA CTCTTCCCAT CAGGTTCCCC TCTATGTGTG AAAAAATGCG CATCCGCCTG
    TTGGACTGGG ACCGCCTCAC CCACAACGAT ATCATCGGCA CCACCTACCT GAGCATGTCC
    AAAATCTCAG CTCCTGGCGG CGAGATTGAA GTTGATGACA CTTTGGGATT TCTGCCAACC
    TTCGGGCCTT GCTATGTGAA CCTTTATGGC AGCCCTCGGG AGTTCACTGG GTTTCCAGAC
    CCTTATGAAG ATCTGAACTT GGGGAAGGGA GAAGGAGTGG CTTACCGTGG TCGGGTCCTT
    CTGGAGTTGG AGACAAAGCT GGTGGAGCAG GCTGAGCAGA AGGTGGAAGA CATTCCTTCA
    GATGATATTT CACGGGTTGA GAAATATCTC CGCAGACGGA AGTACTGCCT CTTTGCCGCT
    TTCTATTCAG CCACCATGTT GCAGGACGTC GATGACGCCA TTCAGTTTGA GGTCAGCATT
    GGAAATTATG GAAACAAGTT CGACAACACC TGCCTCCCCC TGGCCTCCAC CACGCAGTAC
    AGCCGGGCTG TGTTCGACGG TTGCCAGTAC TATTATCTCC CCTGGGGTAA CGTAAAGCCC
    GTTGTTGTTC TCTCATCTCA CTGGGAAGAC ATCGGCCACA GAACTGACAC TCAGAATATT
    CTACAATACG CCATCGATAA TCTGGCAGCG AATCTGGACT GGGTCCACCT TGCTGTGAAA
    GCTAAGAGCT CCCCCTACGA CTTGGACGCT TTGGTCGCGC GTTTGTTTGA TGACATCATC
    ACCGACTGCA GCCACGCCCT GCCAGATGTC AAGTCTATGC AAGGGACCAC CCACCTGGAC
    CAGTACTTGT ACAGATTCCG CAAGACCAAC CTTGCCCAGA TTGTCCAGGC GGCTCTGAAA
    CTCAAGCACG ATGACTGTGT GGAGCAGGAG AATGTGATGG AGCAGGCAGA GGACTGGCTA
    ATGCGACTAA AGGCTATGGC TGAGGAGCCG CAGAACAGCC TGCCAGACAT AGTGATTTGG
    ATGTTACAAG GGGAGAAGCG TGTGGCTTAT TATAGAATGC CGGCCCGCGA TGTCCTTTTC
    TCCAGGAATG GCTCCAAGTG TTGCGGCAAG AACTGCGGGA AGCTCCAGAC AATATTTCTC
    AAATTTCCTC AGGAAGGAAC CCAAGGAGCG AAGATGCCAG CTCAGGTTCG AGTCCAGCTG
    TGGTTTGGGC TCGCTGTGGA TCAGAAGGAA TTCAATCATT ATGCTCAGGG GAAATTGTCT
    GTTTTTGCTG AAGCTTATGA AAACCAAACA AAGCTGGCTC TCGTGGGCAA CTGGGGAACA
    ACTGGCCTTA CGTACCCCAA ATACTCAGAC GTGACCGGGA AAATCAAACT TCCAAAGGAG
    AGTTTCAAGG CTTCCACTGG ATGGAGCTGG GCCGGAGACT GGTTTATCAG CCCGGAGAAA
    ACGTTGCTGT ATGATACGGA CGCGGGTCAC ATGAGCTTCG TGGAAGAGGT TTTCGAGAAC
    CAGGCCAGGC TGCCCGGTGG GCAATGGATC CATATGACGG AGGCCTACTC AGATGTGAAT
    GGGGAGAAGG TTTTGCCAAA AGATGAGATC GAATGCCCCC CTGGCTGGAA ATGGGAGGAT
    GATGAATGGT CCACGGACAC CAACAGGGCT GTTGATGAGC AAGGATGGGA GTACGGCATT
    ACTATCCCCC CTGACCGGAA GCCTAAATCC TGGGTTCCAG CAGAGAAGAT GTATCACACC
    AACCGCAGGA GGCGCTGGGT CAGACTAAGA CGAAGGGACC CCAAGCAGAT TGAGATCCTA
    AAAAAGCACA AGCCGGACGA GTCGGAGAGC GAGGGATGGG AATACGCCTC TCTCTTTGGC
    TGGAAGTTTC ACCTGAAGTA CCGTAAGACT GACAGTTTCC GCAGGCGCAG GTGGAGGCGC
    CGTATGGAAC CCCTGGAACG CACCGGGGCG GCAGCTGTTT TCGCTTTGGA AGGTTCCCTG
    GGTGGAATTA CAGATGATAA AAGCGATGAT GGAAAAAACA CGTCCACAAT GAGCTTCGGG
    GTCAACCGGC CGACGATATC CTGCATATTT GACTATGGGA ACCGTTACCA TTTGCGCTGC
    TACATATACC AAGCGAGGAA CCTGGTGCCC ATGGACCAAG ACAGCTTTTC TGATCCATAC
    GCCATAGTCT CTTTCCTCCA TCAAAGTCAA AAGACCTTGG TGATGAAGAG CACCTTGAAC
    CCAACATGGG ACCAGACCCT TATTTTTCAT GAAATTGAAG TTTTTGGAGA CCCTGAAACC
    ATAAAAGAAT CCCCTCCCAA CATAGTGGTG GAAATCTATG ACCACGATAC TTACGGTGCA
    GATGAATACA TGGGAAGGTG CATTTGCAAG CCAAGCTTTG AGCGCTCCCC CCGGTTATCT
    TGGCACCCGG TGATCATGGG CACGAAGAAC GTCGGAGAGC TGCTCGCTGC CTTCGAGCTC
    ATTCCCAGGG AAAAGCCGGC TGTTCATCTT ATCCCAGGAC TGGAGGGAGA AATCTCAGGA
    GTTCTGAGCG AGCTGCTTAT TCCTCGCTTC TTCACCGAGA AACTTCTTGA CTGGTCATCA
    GACGCCGACC TTCCCTACCC ACCTCCACAG AGGGAGGCCA ATGTGTACAT GGTGCCCCAG
    GGAATCAAAC CCGTTCTGCA GCGCACCGCT ATAGAGATCC TGGCCTGGGG GCTGAGGAAC
    CTAAAGAGCT ACCAGCTGGC CAGCGTGACC TCCCCGAGTC TGATAGTGGA GTGCGGCGAT
    GAACTGATCC AGTCGGGGGT CATTAAGAAC GTCAAGAAGA ACCCCAACTT TGATGTGTGC
    CTGCTCCTCA TGGAAGTGAG GTTGCCCAGA GAAGATCTAT ATTGTCCACC CATCATTGTG
    AAGGTCATCG ATAACCGTCA GTTTGGAAGA AAACCAGTCG TCGGCCAATG CACCATCAAG
    TCTTTGGAAG AGTTCCATTG TGACCCCAAC TCAGAAGATC TAGAGGTAGA AGATGGACTG
    TCAGACAATG TGAGTGTTAC ACCCAGGGAC GAGGTGCTTA TTGACATTGA TGATAAGGAA
    CCTCTTATCC CGACTCAGGA AGAAGAGTTC ATTGATTGGT GGAGTAAGTT TTATGCTTCC
    ATTGGGGAAA AAGAGAAATG TGGAACATAC CTAGAGAAAG GCTTTGATAC CTTGCAGGTA
    CTGGACACAG AGCTAGAGAA CATAGAGGAT TACGAAGAAC TCTCAGACTT TTGCCGAACC
    TTCAAACTGT ACAGAGGAAA AACACAAGAT GAGACAGAAG ATCCCTCTGT GGTTGGAGAA
    TTTAAGGGAT CCTTTAAAAT CTATCCTCTT CCTGATGACC CATCAGTCCC CAGGCCAGCA
    CCCCAGTTCC GCCAACTCCC TGCCAAAGGT CTTCAAGAGT GCCTCGTGCG TGTCTATATT
    GTTCGTGCTT TTGGTTTGCA GCCGAAAGAC CCCAACGGCA AGTGTGATCC CTACGTGAAG
    ATTTCCATTG GAAAGAAGTC ATTAAATGAT CAGGAACATT ATATCCCTTG CACCTTGGAG
    CCTGTTTTTG GAAAAATGTT TGAGCTGACC TGCACCCTCC CTCTGGAGAA AGACCTGAAG
    GTCACACTCT ATGATTATGA CATGATTTCA AAAGATGAGA AGATTGGAGA GACGGTGATA
    GACCTGGAGA ACCGTTTCCT CTCGAAATAT GGAGCAAGAT GTGGATTGCC TCAATCCTAC
    TGCATATCTG GGCCAAACCA GTGGCGGGAC CAACGGAAAC CATCGGAACT TCTCAGAATC
    TTTGCGCAGC AGCGGAACTT CAAACCCCCT AACTTCAAAA ACAACAGCAT AATGTTCCGG
    AACCATGAGT ACCATGTCTC TGAGCTGGAA GACAGCAAGT TCCCTAACCC GCACCAGGGC
    CCGCCGGAGG AACGGCTCGC TCTACACGTA CTGAGAAGGC AAGGCCTGGT GTGTGAGCAT
    GTGGAGACCA GGGCCCTGTA CAGCCCTTTG CAGCCGGATA TAGAACAGGG AAAGCTGCAG
    ATGTGGGTTG ACTTGTTCCC CAAGTCCCTC GGTTCTCCCG GCCCTCCCTT TAATATAACC
    CCACGGAAAG CCAAACGGTA TTACTTGCGC TGTATCATCT GGAACACCAA AGATGTGATT
    CTGGATGATG TGAGCATCAC AGGAGAGAAG ATGAGTGATA TCTATGTCAA GAGCTGGCTG
    ATTGGCCATG AAGAAAATAA ACAGAAGACA GATGTTCACT ACAGGTCTAT GGGAGGTGAA
    GGGAATTTTA ACTGGCGGCT TGTGTTCCCT TTCGACTATC TTCCCGCGGA GCAGGTCTGC
    TCAGTGTCCA GAAAGGAGCA TTTCTGGAGC CTCGACAAGA CTGAGAATAA AATCCCTCCA
    AACCTCATTA TACAGATATG GGACAACGAT AAGTTCTCCT TCGATGACTA TTTAGGTTCC
    GTGCAGCTGG ACCTCAACCG GATGCCACGA CCTGCCAAAA CGGCGGAGAA GTGCAGGCTG
    GACCTGCTGG ATGAGGTGGA GTCTGCAAAT CACTGCTCCT TGTTTGAGCA GAAGACAGTC
    AAGGGATGGT GGCCGTGTGT AGGGCAGGAG GGGGACGAGA AGATTCTGGC TGGAAAAGTG
    GAAATTACTT TAGAGATTGT AAATGAACAG GAACATGATG AGAGACCGGC CGGGATTGGT
    CGAGATGAGC CAAATATGAA CCCAAGACTT GAAGACCCAA AGCGCCCAGA AACCTCGTTC
    CTCTGGTTCA CTTCCCCGTA CAAGACCCTG AAGTACATCC TGTGGAGGAG ATACAAGTGG
    CTCATCCTTG TCTTCTTCCT TCTATTCATC CTACTGCTCT TCCTCGGAAT CTTCCTGTAT
    TCCTTCCCGA ACTACGCTGC TATGAAGCTG GTGAAGCCGT TCAGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa49542
    Clone ID Related Accession (Same CDS sequence) XM_031895269.1
    Accession Version XM_031895269.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product dysferlin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTGAGGG TCTTTATCCT TCATGCTGAA AATGTGCAAA CCCCAGACTC AGACATCAGC 
    GACTCCTACT GCTCTGTGGT GTTCCAAGGG GTTAAGAAGA AAACGAAAAT CATAAAGAAC
    AATCTGAATC CCATATGGAA CGAGGGATTC GAGTGGGATT TAAAAGGATT GCCCTTGGAC
    AACAGTTCGG AGCTTGTCGC GGTAGTGAAA GACCATGAAA CCATGGGAAG GAACAGGTTT
    CTGGGTGAGA CCCGAGTCAG CCTACGGGAT TTACTTACGT CCCCTAACCT GACGGCCACG
    TATAATGCCC CACTCCTGGA CAGCAAGAAG CAGGGCACGG GGGCGATATT AACCTTGCAG
    ATATCTTACT TTCCTCCACC GGGGGCAGCC CCAGTTGTAC CCCCTCCTGC ACCCAAGGAG
    CCCACCCCAT CGCTCAATGA GTTGGACACC ATCACTGATA CGGCTGGTGA AGAGGACACG
    GAAGACCAGG CTGGAACTGG CGATGAAGCG GATATTGTCT CTCTGGCCCC TGGAGGAGCA
    GAGCAACCCA CTCTCCCCAA GAAAACTGCC CCTGTCCCTG TGCAGGGATT AAAGAGAAAG
    AAGAGCAACT CCAAAAAACA CCTCTCCAAC AAGCCTCAGG ACTTCCAGGT TCGTGTTCGA
    GTGATAGAAG GGAGGCAGAT CCCAGGGATT AACATCAAAC CTGTTGTCAA AGTCACAGTT
    GCTGGGCAGA CCAAGAGAAC CCGGATACGC AAAGGGAACA ACCCATATTT CGATGAGACA
    TTTTTCTTCA ATTTTTTTGA GTCCCCTTCT GAACTCTTTG ATGAACCCGT GTTCCTGACT
    GTTCTCGATT CCCGCTCCCT CCGAACAGAC TCAGTGATTG GTGAATTCCG GATGGACGTC
    GGAATGATTT ATGGAGAATC AAAACATGCC TTCCTCAGGA AGTGGCTTCT CCTCTCCGAC
    CCAGACGATT TCTCCGCGGG GGCCAAGGGG TACCTGAAGG TCAGCCTCTA TGTACTTGGG
    CCTGGAGATG AGAGCCCGGT AGAGAAAAAG GAGGTCTCGG AAGACAAGGA GGACATTGAG
    GGGAACTTGC TGCGACCGGC CGGGGTGTCC CTGCGAGGAG CCCACCTTAC TCTGAAGATT
    TACAGGGCGG AAGATCTGCC GCAGATGGAC GATGCTGTGC TGGACAATGT CAAACAGATC
    TTTGGCTTTG ACAGCAACAA GAAAAACTTG GTGGATCCCT TTGTGGAAGT CAGTTTTGCG
    GGGAAGATGC TCCTCAGTAA GATCATGGAG AAGAACGCCA ACCCGCAGTG GAGTCAGTCC
    ATCACTCTTC CCATCAGGTT CCCCTCTATG TGTGAAAAAA TGCGCATCCG CCTGTTGGAC
    TGGGACCGCC TCACCCACAA CGATATCATC GGCACCACCT ACCTGAGCAT GTCCAAAATC
    TCAGCTCCTG GCGGCGAGAT TGAAGTTGAT GACACTTTGG GATTTCTGCC AACCTTCGGG
    CCTTGCTATG TGAACCTTTA TGGCAGCCCT CGGGAGTTCA CTGGGTTTCC AGACCCTTAT
    GAAGATCTGA ACTTGGGGAA GGGAGAAGGA GTGGCTTACC GTGGTCGGGT CCTTCTGGAG
    TTGGAGACAA AGCTGGTGGA GCAGGCTGAG CAGAAGGTGG AAGACATTCC TTCAGATGAT
    ATTTCACGGG TTGAGAAATA TCTCCGCAGA CGGAAGTACT GCCTCTTTGC CGCTTTCTAT
    TCAGCCACCA TGTTGCAGGA CGTCGATGAC GCCATTCAGT TTGAGGTCAG CATTGGAAAT
    TATGGAAACA AGTTCGACAA CACCTGCCTC CCCCTGGCCT CCACCACGCA GTACAGCCGG
    GCTGTGTTCG ACGGTTGCCA GTACTATTAT CTCCCCTGGG GTAACGTAAA GCCCGTTGTT
    GTTCTCTCAT CTCACTGGGA AGACATCGGC CACAGAACTG ACACTCAGAA TATTCTACAA
    TACGCCATCG ATAATCTGGC AGCGAATCTG GACTGGGTCC ACCTTGCTGT GAAAGCTAAG
    AGCTCCCCCT ACGACTTGGA CGCTTTGGTC GCGCGTTTGT TTGATGACAT CATCACCGAC
    TGCAGCCACG CCCTGCCAGA TGTCAAGTCT ATGCAAGGGA CCACCCACCT GGACCAGTAC
    TTGTACAGAT TCCGCAAGAC CAACCTTGCC CAGATTGTCC AGGCGGCTCT GAAACTCAAG
    CACGATGACT GTGTGGAGCA GGAGAATGTG ATGGAGCAGG CAGAGGACTG GCTAATGCGA
    CTAAAGGCTA TGGCTGAGGA GCCGCAGAAC AGCCTGCCAG ACATAGTGAT TTGGATGTTA
    CAAGGGGAGA AGCGTGTGGC TTATTATAGA ATGCCGGCCC GCGATGTCCT TTTCTCCAGG
    AATGGCTCCA AGTGTTGCGG CAAGAACTGC GGGAAGCTCC AGACAATATT TCTCAAATTT
    CCTCAGGAAG GAACCCAAGG AGCGAAGATG CCAGCTCAGG TTCGAGTCCA GCTGTGGTTT
    GGGCTCGCTG TGGATCAGAA GGAATTCAAT CATTATGCTC AGGGGAAATT GTCTGTTTTT
    GCTGAAGCTT ATGAAAACCA AACAAAGCTG GCTCTCGTGG GCAACTGGGG AACAACTGGC
    CTTACGTACC CCAAATACTC AGACGTGACC GGGAAAATCA AACTTCCAAA GGAGAGTTTC
    AAGGCTTCCA CTGGATGGAG CTGGGCCGGA GACTGGTTTA TCAGCCCGGA GAAAACGTTG
    CTGTATGATA CGGACGCGGG TCACATGAGC TTCGTGGAAG AGGTTTTCGA GAACCAGGCC
    AGGCTGCCCG GTGGGCAATG GATCCATATG ACGGAGGCCT ACTCAGATGT GAATGGGGAG
    AAGGTTTTGC CAAAAGATGA GATCGAATGC CCCCCTGGCT GGAAATGGGA GGATGATGAA
    TGGTCCACGG ACACCAACAG GGCTGTTGAT GAGCAAGGAT GGGAGTACGG CATTACTATC
    CCCCCTGACC GGAAGCCTAA ATCCTGGGTT CCAGCAGAGA AGATGTATCA CACCAACCGC
    AGGAGGCGCT GGGTCAGACT AAGACGAAGG GACCCCAAGC AGATTGAGAT CCTAAAAAAG
    CACAAGCCGG ACGAGTCGGA GAGCGAGGGA TGGGAATACG CCTCTCTCTT TGGCTGGAAG
    TTTCACCTGA AGTACCGTAA GACTGACAGT TTCCGCAGGC GCAGGTGGAG GCGCCGTATG
    GAACCCCTGG AACGCACCGG GGCGGCAGCT GTTTTCGCTT TGGAAGGTTC CCTGGGTGGA
    ATTACAGATG ATAAAAGCGA TGATGGAAAA AACACGTCCA CAATGAGCTT CGGGGTCAAC
    CGGCCGACGA TATCCTGCAT ATTTGACTAT GGGAACCGTT ACCATTTGCG CTGCTACATA
    TACCAAGCGA GGAACCTGGT GCCCATGGAC CAAGACAGCT TTTCTGATCC ATACGCCATA
    GTCTCTTTCC TCCATCAAAG TCAAAAGACC TTGGTGATGA AGAGCACCTT GAACCCAACA
    TGGGACCAGA CCCTTATTTT TCATGAAATT GAAGTTTTTG GAGACCCTGA AACCATAAAA
    GAATCCCCTC CCAACATAGT GGTGGAAATC TATGACCACG ATACTTACGG TGCAGATGAA
    TACATGGGAA GGTGCATTTG CAAGCCAAGC TTTGAGCGCT CCCCCCGGTT ATCTTGGCAC
    CCGGTGATCA TGGGCACGAA GAACGTCGGA GAGCTGCTCG CTGCCTTCGA GCTCATTCCC
    AGGGAAAAGC CGGCTGTTCA TCTTATCCCA GGACTGGAGG GAGAAATCTC AGGAGTTCTG
    AGCGAGCTGC TTATTCCTCG CTTCTTCACC GAGAAACTTC TTGACTGGTC ATCAGACGCC
    GACCTTCCCT ACCCACCTCC ACAGAGGGAG GCCAATGTGT ACATGGTGCC CCAGGGAATC
    AAACCCGTTC TGCAGCGCAC CGCTATAGAG ATCCTGGCCT GGGGGCTGAG GAACCTAAAG
    AGCTACCAGC TGGCCAGCGT GACCTCCCCG AGTCTGATAG TGGAGTGCGG CGATGAACTG
    ATCCAGTCGG GGGTCATTAA GAACGTCAAG AAGAACCCCA ACTTTGATGT GTGCCTGCTC
    CTCATGGAAG TGAGGTTGCC CAGAGAAGAT CTATATTGTC CACCCATCAT TGTGAAGGTC
    ATCGATAACC GTCAGTTTGG AAGAAAACCA GTCGTCGGCC AATGCACCAT CAAGTCTTTG
    GAAGAGTTCC ATTGTGACCC CAACTCAGAA GATCTAGAGG TAGAAGATGG ACTGTCAGAC
    AATGTGAGTG TTACACCCAG GGACGAGGTG CTTATTGACA TTGATGATAA GGAACCTCTT
    ATCCCGACTC AGGAAGAAGA GTTCATTGAT TGGTGGAGTA AGTTTTATGC TTCCATTGGG
    GAAAAAGAGA AATGTGGAAC ATACCTAGAG AAAGGCTTTG ATACCTTGCA GGTACTGGAC
    ACAGAGCTAG AGAACATAGA GGATTACGAA GAACTCTCAG ACTTTTGCCG AACCTTCAAA
    CTGTACAGAG GAAAAACACA AGATGAGACA GAAGATCCCT CTGTGGTTGG AGAATTTAAG
    GGATCCTTTA AAATCTATCC TCTTCCTGAT GACCCATCAG TCCCCAGGCC AGCACCCCAG
    TTCCGCCAAC TCCCTGCCAA AGGTCTTCAA GAGTGCCTCG TGCGTGTCTA TATTGTTCGT
    GCTTTTGGTT TGCAGCCGAA AGACCCCAAC GGCAAGTGTG ATCCCTACGT GAAGATTTCC
    ATTGGAAAGA AGTCATTAAA TGATCAGGAA CATTATATCC CTTGCACCTT GGAGCCTGTT
    TTTGGAAAAA TGTTTGAGCT GACCTGCACC CTCCCTCTGG AGAAAGACCT GAAGGTCACA
    CTCTATGATT ATGACATGAT TTCAAAAGAT GAGAAGATTG GAGAGACGGT GATAGACCTG
    GAGAACCGTT TCCTCTCGAA ATATGGAGCA AGATGTGGAT TGCCTCAATC CTACTGCATA
    TCTGGGCCAA ACCAGTGGCG GGACCAACGG AAACCATCGG AACTTCTCAG AATCTTTGCG
    CAGCAGCGGA ACTTCAAACC CCCTAACTTC AAAAACAACA GCATAATGTT CCGGAACCAT
    GAGTACCATG TCTCTGAGCT GGAAGACAGC AAGTTCCCTA ACCCGCACCA GGGCCCGCCG
    GAGGAACGGC TCGCTCTACA CGTACTGAGA AGGCAAGGCC TGGTGTGTGA GCATGTGGAG
    ACCAGGGCCC TGTACAGCCC TTTGCAGCCG GATATAGAAC AGGGAAAGCT GCAGATGTGG
    GTTGACTTGT TCCCCAAGTC CCTCGGTTCT CCCGGCCCTC CCTTTAATAT AACCCCACGG
    AAAGCCAAAC GGTATTACTT GCGCTGTATC ATCTGGAACA CCAAAGATGT GATTCTGGAT
    GATGTGAGCA TCACAGGAGA GAAGATGAGT GATATCTATG TCAAGAGCTG GCTGATTGGC
    CATGAAGAAA ATAAACAGAA GACAGATGTT CACTACAGGT CTATGGGAGG TGAAGGGAAT
    TTTAACTGGC GGCTTGTGTT CCCTTTCGAC TATCTTCCCG CGGAGCAGGT CTGCTCAGTG
    TCCAGAAAGG AGCATTTCTG GAGCCTCGAC AAGACTGAGA ATAAAATCCC TCCAAACCTC
    ATTATACAGA TATGGGACAA CGATAAGTTC TCCTTCGATG ACTATTTAGG TTCCGTGCAG
    CTGGACCTCA ACCGGATGCC ACGACCTGCC AAAACGGCGG AGAAGTGCAG GCTGGACCTG
    CTGGATGAGG TGGAGTCTGC AAATCACTGC TCCTTGTTTG AGCAGAAGAC AGTCAAGGGA
    TGGTGGCCGT GTGTAGGGCA GGAGGGGGAC GAGAAGATTC TGGCTGGAAA AGTGGAAATT
    ACTTTAGAGA TTGTAAATGA ACAGGAACAT GATGAGAGAC CGGCCGGGAT TGGTCGAGAT
    GAGCCAAATA TGAACCCAAG ACTTGAAGAC CCAAAGCGCC CAGAAACCTC GTTCCTCTGG
    TTCACTTCCC CGTACAAGAC CCTGAAGTAC ATCCTGTGGA GGAGATACAA GTGGCTCATC
    CTTGTCTTCT TCCTTCTATT CATCCTACTG CTCTTCCTCG GAATCTTCCT GTATTCCTTC
    CCGAACTACG CTGCTATGAA GCTGGTGAAG CCGTTCAGTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751129.1
    CDS138..6359
    Translation

    Target ORF information:

    RefSeq Version XM_031895269.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis dysferlin (LOC100488564), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031895269.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTGAGGG TCTTTATCCT TCATGCTGAA AATGTGCAAA CCCCAGACTC AGACATCAGC 
    GACTCCTACT GCTCTGTGGT GTTCCAAGGG GTTAAGAAGA AAACGAAAAT CATAAAGAAC
    AATCTGAATC CCATATGGAA CGAGGGATTC GAGTGGGATT TAAAAGGATT GCCCTTGGAC
    AACAGTTCGG AGCTTGTCGC GGTAGTGAAA GACCATGAAA CCATGGGAAG GAACAGGTTT
    CTGGGTGAGA CCCGAGTCAG CCTACGGGAT TTACTTACGT CCCCTAACCT GACGGCCACG
    TATAATGCCC CACTCCTGGA CAGCAAGAAG CAGGGCACGG GGGCGATATT AACCTTGCAG
    ATATCTTACT TTCCTCCACC GGGGGCAGCC CCAGTTGTAC CCCCTCCTGC ACCCAAGGAG
    CCCACCCCAT CGCTCAATGA GTTGGACACC ATCACTGATA CGGCTGGTGA AGAGGACACG
    GAAGACCAGG CTGGAACTGG CGATGAAGCG GATATTGTCT CTCTGGCCCC TGGAGGAGCA
    GAGCAACCCA CTCTCCCCAA GAAAACTGCC CCTGTCCCTG TGCAGGGATT AAAGAGAAAG
    AAGAGCAACT CCAAAAAACA CCTCTCCAAC AAGCCTCAGG ACTTCCAGGT TCGTGTTCGA
    GTGATAGAAG GGAGGCAGAT CCCAGGGATT AACATCAAAC CTGTTGTCAA AGTCACAGTT
    GCTGGGCAGA CCAAGAGAAC CCGGATACGC AAAGGGAACA ACCCATATTT CGATGAGACA
    TTTTTCTTCA ATTTTTTTGA GTCCCCTTCT GAACTCTTTG ATGAACCCGT GTTCCTGACT
    GTTCTCGATT CCCGCTCCCT CCGAACAGAC TCAGTGATTG GTGAATTCCG GATGGACGTC
    GGAATGATTT ATGGAGAATC AAAACATGCC TTCCTCAGGA AGTGGCTTCT CCTCTCCGAC
    CCAGACGATT TCTCCGCGGG GGCCAAGGGG TACCTGAAGG TCAGCCTCTA TGTACTTGGG
    CCTGGAGATG AGAGCCCGGT AGAGAAAAAG GAGGTCTCGG AAGACAAGGA GGACATTGAG
    GGGAACTTGC TGCGACCGGC CGGGGTGTCC CTGCGAGGAG CCCACCTTAC TCTGAAGATT
    TACAGGGCGG AAGATCTGCC GCAGATGGAC GATGCTGTGC TGGACAATGT CAAACAGATC
    TTTGGCTTTG ACAGCAACAA GAAAAACTTG GTGGATCCCT TTGTGGAAGT CAGTTTTGCG
    GGGAAGATGC TCCTCAGTAA GATCATGGAG AAGAACGCCA ACCCGCAGTG GAGTCAGTCC
    ATCACTCTTC CCATCAGGTT CCCCTCTATG TGTGAAAAAA TGCGCATCCG CCTGTTGGAC
    TGGGACCGCC TCACCCACAA CGATATCATC GGCACCACCT ACCTGAGCAT GTCCAAAATC
    TCAGCTCCTG GCGGCGAGAT TGAAGTTGAT GACACTTTGG GATTTCTGCC AACCTTCGGG
    CCTTGCTATG TGAACCTTTA TGGCAGCCCT CGGGAGTTCA CTGGGTTTCC AGACCCTTAT
    GAAGATCTGA ACTTGGGGAA GGGAGAAGGA GTGGCTTACC GTGGTCGGGT CCTTCTGGAG
    TTGGAGACAA AGCTGGTGGA GCAGGCTGAG CAGAAGGTGG AAGACATTCC TTCAGATGAT
    ATTTCACGGG TTGAGAAATA TCTCCGCAGA CGGAAGTACT GCCTCTTTGC CGCTTTCTAT
    TCAGCCACCA TGTTGCAGGA CGTCGATGAC GCCATTCAGT TTGAGGTCAG CATTGGAAAT
    TATGGAAACA AGTTCGACAA CACCTGCCTC CCCCTGGCCT CCACCACGCA GTACAGCCGG
    GCTGTGTTCG ACGGTTGCCA GTACTATTAT CTCCCCTGGG GTAACGTAAA GCCCGTTGTT
    GTTCTCTCAT CTCACTGGGA AGACATCGGC CACAGAACTG ACACTCAGAA TATTCTACAA
    TACGCCATCG ATAATCTGGC AGCGAATCTG GACTGGGTCC ACCTTGCTGT GAAAGCTAAG
    AGCTCCCCCT ACGACTTGGA CGCTTTGGTC GCGCGTTTGT TTGATGACAT CATCACCGAC
    TGCAGCCACG CCCTGCCAGA TGTCAAGTCT ATGCAAGGGA CCACCCACCT GGACCAGTAC
    TTGTACAGAT TCCGCAAGAC CAACCTTGCC CAGATTGTCC AGGCGGCTCT GAAACTCAAG
    CACGATGACT GTGTGGAGCA GGAGAATGTG ATGGAGCAGG CAGAGGACTG GCTAATGCGA
    CTAAAGGCTA TGGCTGAGGA GCCGCAGAAC AGCCTGCCAG ACATAGTGAT TTGGATGTTA
    CAAGGGGAGA AGCGTGTGGC TTATTATAGA ATGCCGGCCC GCGATGTCCT TTTCTCCAGG
    AATGGCTCCA AGTGTTGCGG CAAGAACTGC GGGAAGCTCC AGACAATATT TCTCAAATTT
    CCTCAGGAAG GAACCCAAGG AGCGAAGATG CCAGCTCAGG TTCGAGTCCA GCTGTGGTTT
    GGGCTCGCTG TGGATCAGAA GGAATTCAAT CATTATGCTC AGGGGAAATT GTCTGTTTTT
    GCTGAAGCTT ATGAAAACCA AACAAAGCTG GCTCTCGTGG GCAACTGGGG AACAACTGGC
    CTTACGTACC CCAAATACTC AGACGTGACC GGGAAAATCA AACTTCCAAA GGAGAGTTTC
    AAGGCTTCCA CTGGATGGAG CTGGGCCGGA GACTGGTTTA TCAGCCCGGA GAAAACGTTG
    CTGTATGATA CGGACGCGGG TCACATGAGC TTCGTGGAAG AGGTTTTCGA GAACCAGGCC
    AGGCTGCCCG GTGGGCAATG GATCCATATG ACGGAGGCCT ACTCAGATGT GAATGGGGAG
    AAGGTTTTGC CAAAAGATGA GATCGAATGC CCCCCTGGCT GGAAATGGGA GGATGATGAA
    TGGTCCACGG ACACCAACAG GGCTGTTGAT GAGCAAGGAT GGGAGTACGG CATTACTATC
    CCCCCTGACC GGAAGCCTAA ATCCTGGGTT CCAGCAGAGA AGATGTATCA CACCAACCGC
    AGGAGGCGCT GGGTCAGACT AAGACGAAGG GACCCCAAGC AGATTGAGAT CCTAAAAAAG
    CACAAGCCGG ACGAGTCGGA GAGCGAGGGA TGGGAATACG CCTCTCTCTT TGGCTGGAAG
    TTTCACCTGA AGTACCGTAA GACTGACAGT TTCCGCAGGC GCAGGTGGAG GCGCCGTATG
    GAACCCCTGG AACGCACCGG GGCGGCAGCT GTTTTCGCTT TGGAAGGTTC CCTGGGTGGA
    ATTACAGATG ATAAAAGCGA TGATGGAAAA AACACGTCCA CAATGAGCTT CGGGGTCAAC
    CGGCCGACGA TATCCTGCAT ATTTGACTAT GGGAACCGTT ACCATTTGCG CTGCTACATA
    TACCAAGCGA GGAACCTGGT GCCCATGGAC CAAGACAGCT TTTCTGATCC ATACGCCATA
    GTCTCTTTCC TCCATCAAAG TCAAAAGACC TTGGTGATGA AGAGCACCTT GAACCCAACA
    TGGGACCAGA CCCTTATTTT TCATGAAATT GAAGTTTTTG GAGACCCTGA AACCATAAAA
    GAATCCCCTC CCAACATAGT GGTGGAAATC TATGACCACG ATACTTACGG TGCAGATGAA
    TACATGGGAA GGTGCATTTG CAAGCCAAGC TTTGAGCGCT CCCCCCGGTT ATCTTGGCAC
    CCGGTGATCA TGGGCACGAA GAACGTCGGA GAGCTGCTCG CTGCCTTCGA GCTCATTCCC
    AGGGAAAAGC CGGCTGTTCA TCTTATCCCA GGACTGGAGG GAGAAATCTC AGGAGTTCTG
    AGCGAGCTGC TTATTCCTCG CTTCTTCACC GAGAAACTTC TTGACTGGTC ATCAGACGCC
    GACCTTCCCT ACCCACCTCC ACAGAGGGAG GCCAATGTGT ACATGGTGCC CCAGGGAATC
    AAACCCGTTC TGCAGCGCAC CGCTATAGAG ATCCTGGCCT GGGGGCTGAG GAACCTAAAG
    AGCTACCAGC TGGCCAGCGT GACCTCCCCG AGTCTGATAG TGGAGTGCGG CGATGAACTG
    ATCCAGTCGG GGGTCATTAA GAACGTCAAG AAGAACCCCA ACTTTGATGT GTGCCTGCTC
    CTCATGGAAG TGAGGTTGCC CAGAGAAGAT CTATATTGTC CACCCATCAT TGTGAAGGTC
    ATCGATAACC GTCAGTTTGG AAGAAAACCA GTCGTCGGCC AATGCACCAT CAAGTCTTTG
    GAAGAGTTCC ATTGTGACCC CAACTCAGAA GATCTAGAGG TAGAAGATGG ACTGTCAGAC
    AATGTGAGTG TTACACCCAG GGACGAGGTG CTTATTGACA TTGATGATAA GGAACCTCTT
    ATCCCGACTC AGGAAGAAGA GTTCATTGAT TGGTGGAGTA AGTTTTATGC TTCCATTGGG
    GAAAAAGAGA AATGTGGAAC ATACCTAGAG AAAGGCTTTG ATACCTTGCA GGTACTGGAC
    ACAGAGCTAG AGAACATAGA GGATTACGAA GAACTCTCAG ACTTTTGCCG AACCTTCAAA
    CTGTACAGAG GAAAAACACA AGATGAGACA GAAGATCCCT CTGTGGTTGG AGAATTTAAG
    GGATCCTTTA AAATCTATCC TCTTCCTGAT GACCCATCAG TCCCCAGGCC AGCACCCCAG
    TTCCGCCAAC TCCCTGCCAA AGGTCTTCAA GAGTGCCTCG TGCGTGTCTA TATTGTTCGT
    GCTTTTGGTT TGCAGCCGAA AGACCCCAAC GGCAAGTGTG ATCCCTACGT GAAGATTTCC
    ATTGGAAAGA AGTCATTAAA TGATCAGGAA CATTATATCC CTTGCACCTT GGAGCCTGTT
    TTTGGAAAAA TGTTTGAGCT GACCTGCACC CTCCCTCTGG AGAAAGACCT GAAGGTCACA
    CTCTATGATT ATGACATGAT TTCAAAAGAT GAGAAGATTG GAGAGACGGT GATAGACCTG
    GAGAACCGTT TCCTCTCGAA ATATGGAGCA AGATGTGGAT TGCCTCAATC CTACTGCATA
    TCTGGGCCAA ACCAGTGGCG GGACCAACGG AAACCATCGG AACTTCTCAG AATCTTTGCG
    CAGCAGCGGA ACTTCAAACC CCCTAACTTC AAAAACAACA GCATAATGTT CCGGAACCAT
    GAGTACCATG TCTCTGAGCT GGAAGACAGC AAGTTCCCTA ACCCGCACCA GGGCCCGCCG
    GAGGAACGGC TCGCTCTACA CGTACTGAGA AGGCAAGGCC TGGTGTGTGA GCATGTGGAG
    ACCAGGGCCC TGTACAGCCC TTTGCAGCCG GATATAGAAC AGGGAAAGCT GCAGATGTGG
    GTTGACTTGT TCCCCAAGTC CCTCGGTTCT CCCGGCCCTC CCTTTAATAT AACCCCACGG
    AAAGCCAAAC GGTATTACTT GCGCTGTATC ATCTGGAACA CCAAAGATGT GATTCTGGAT
    GATGTGAGCA TCACAGGAGA GAAGATGAGT GATATCTATG TCAAGAGCTG GCTGATTGGC
    CATGAAGAAA ATAAACAGAA GACAGATGTT CACTACAGGT CTATGGGAGG TGAAGGGAAT
    TTTAACTGGC GGCTTGTGTT CCCTTTCGAC TATCTTCCCG CGGAGCAGGT CTGCTCAGTG
    TCCAGAAAGG AGCATTTCTG GAGCCTCGAC AAGACTGAGA ATAAAATCCC TCCAAACCTC
    ATTATACAGA TATGGGACAA CGATAAGTTC TCCTTCGATG ACTATTTAGG TTCCGTGCAG
    CTGGACCTCA ACCGGATGCC ACGACCTGCC AAAACGGCGG AGAAGTGCAG GCTGGACCTG
    CTGGATGAGG TGGAGTCTGC AAATCACTGC TCCTTGTTTG AGCAGAAGAC AGTCAAGGGA
    TGGTGGCCGT GTGTAGGGCA GGAGGGGGAC GAGAAGATTC TGGCTGGAAA AGTGGAAATT
    ACTTTAGAGA TTGTAAATGA ACAGGAACAT GATGAGAGAC CGGCCGGGAT TGGTCGAGAT
    GAGCCAAATA TGAACCCAAG ACTTGAAGAC CCAAAGCGCC CAGAAACCTC GTTCCTCTGG
    TTCACTTCCC CGTACAAGAC CCTGAAGTAC ATCCTGTGGA GGAGATACAA GTGGCTCATC
    CTTGTCTTCT TCCTTCTATT CATCCTACTG CTCTTCCTCG GAATCTTCCT GTATTCCTTC
    CCGAACTACG CTGCTATGAA GCTGGTGAAG CCGTTCAGTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.