tbc1d32 cDNA ORF clone, Amphiprion ocellaris(clown anemonefish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tbc1d32
Entrez Gene ID	111564607
Full Name	TBC1 domain family member 32
Gene Type	protein-coding
Organism	Amphiprion ocellaris(clown anemonefish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023264284.1	XP_023120052.1	protein broad-minded
XM_023264286.1	XP_023120054.1	protein broad-minded

Pathways from BioSystems
Gene Ontology

The following tbc1d32 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tbc1d32 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAm103566	XM_023264286.1 Latest version!	Amphiprion ocellaris TBC1 domain family member 32 (tbc1d32), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OAm103566	XM_023264284.1 Latest version!	Amphiprion ocellaris TBC1 domain family member 32 (tbc1d32), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tbc1d32 ( XM_023264284.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023120052.1 Amphiprion ocellaris TBC1 domain family member 32 (tbc1d32), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAm103566
Clone ID Related Accession (Same CDS sequence)	XM_023264284.1 , XM_023264286.1
Accession Version	XM_023264284.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3180bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	protein broad-minded
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019376179.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGT TATCAGCAGA AGATGAGGTG GAGTTACAGT CCCTCCTCAA GCAGCTTCTG 
CAAAGTATCA ATGAAAAAAT TGCTGGTGCG CCGTCAACTG AATGTGCTGA AGAGATCCTA 
CTTCATCTGG AGGAGACTGA CAAGAACTTC CACAATTATG AGTTTGTGAA GTACCTGCGT 
CAGTATGTGG AGAACTCGCT GGGGACTATG GTGGAAGAGG AGACAAAGAG CTTATGCAGA 
GGAGATGGGC AACATGCAAT CGGTTCAGGA CATGACACCC TGATTCACGC TGTGACTCGA 
AGGACCAGAG ACTCAGCTGA GTATCAGCAG ATGATGCAGA CATTGAAAAA CACCATGATC 
ATGGTTGTGG AGTCTTTAAT CAACAAAATT GAGGAGGACC AGCTGAGGAA GGAGGAGATG 
CACCGGGAGA AGCAGCACAG TCAGTCCAAC AGCCAGTACA CTGATAACTG CTCTGACAGC 
GACTCCTCCT TCAACCAGAG TTATGCATTC ATCAGACAAG AGCAGCTGCA AGTTTTGGCT 
GAAAAACTTG AGTCCAGCAA GCCCAAAGAG GTGCGTTGGG AGGCCCTGCA GGCCCTCTGC 
TGTGCGCCTC CATCCGATGT GCTGAGCTGT GAGAGCTGGA GCAGCCTGCG CAGAAACCTC 
TGTGCGGCTC TGACTGAACC AGACCCCGAC CTCAGCGAGA ATGTACTGCA GTTCTTTGCT 
AAAAGCTTCT CGTCCTCTCC ACTGAATGTG ACACGAGAGA TCTACACCAG CTTGGCGACA 
AGTGTGGACT CAAGCTTTCT GTCTCAGAAG TTGAGCTTTC CAAGTGGCTC AGCTGTTCTC 
GACATCAACA GACCTGAGAT CAGTCGGCTT CTCAAACAGA TTCGATTGAT GAATGACTTT 
CAAAAGGAGG TGACTAACTT TTGGATCCGT CATCCAGAGA AATATATGGA GGAAGTTATA 
GAGAGCACGT TCTTCCTGTT ATCCTTCCAT CATGAACATG GCTCAGCAGG AACAGAGAAA 
GCCCTGGAAC CAATTCACTT ACTGGCCTTA CTGGATGTTA AAGCTACCTG GTTTAAAAAA 
TGGATGCATG GCTACTACAG CAGGACTGTG GTTCTCAGAC TCCTGGAGAG AAAATACAAG 
TCTTTGGTTG TAGCAGCTCT TCAGCAGTGT ATCCATTACA TTGAATCCTG TGAACATTTC 
ACAAATGAGG TAACTGATCC CCAGTCAGAG GAGCAGCAGA ACTCTGGTTC AGTGCAGAGG 
AGTGTTTATT CGGGAAAGGA GCTTGAATAT TCCTTCTTTG TCCACTCTCT GTGTGTCCTT 
GGGAAACTTA TCATCTACAC CAATGGAAGA AAGCTGTTTC CCATCAAAAT GAGGAAGCAC 
AAAGATCCAG TCAGCCTGAC AGACCTGGTT GTCATCCTTA TTAAGATCAT GTATCGGCAC 
CCCCAGCCTC TGCACAGTGA TACCTCTCAC ACAGACTCCC TGTCGCCCAG CAGTCTGGTG 
ATGGAGGTGC TGTGGATGCT ATGTGAACGG ACAGAGTGTG CTGCAGAGTG TCTCTACCAG 
ACTCCTGTCA TAGAGACGCT GCTGGCTCCG GTTATAGCTC TGCTCAATGG ACAGCAGTCC 
CAATTAAAGT CTCCTGCAGC AACACTGACT CTCATTGCTG ACGTTCTGGC TCGCATCGCA 
AACACTGACA GAGGATTAGC TCTCTTCTTA TATGAGAAGA ATCTCGTTCT AGCCCACAGT 
GAGAGAACCT TTGCTGCTCA TGTTATTGTG CAGTTCACCC TGAGGCTGCT GGATAAGGGG 
CTACCATCAC TAAGTGAATC AGATGTGTCC CATACATTAT GTGGAGCCTT TATCTTTGTG 
TGTCGGCAGA TATACAACAC CTGTGAGGGT CTGCAGGTTC TCCGACCCTA CGGGCTGCAC 
AAAGCTGTAG CGGCTGCATG GAAACAGACT AGTTCTTTGT CAGAAAGGGT TCCAACGCCA 
GTACCTGGAG CCAGTGCTGG AACATCTACC CAAGAGCTGC AGCACATTCA CATCTGGGAG 
GAGACATTGT TGGACAGTCT GCTGAACTTT GCAGCAACTC CTAAAGGCCT GCTGCTGCTC 
CAGCAGACAG GAGCCATTAA TGAGTGTGTC TCCTACATGT TCTCTCGCTT TACCAAAAAG 
CTACAGGTGA GCCGCTGTGA GAAGTTTGGA TATGGGGTGA TGGTGACACA GGTGGCAGCA 
ACTGCTCCAG GGGTCGTGGC TCTGCACAGC TCTGGTTTTG TCCGGGCACT GGTAGTAGAG 
CTGTGGTCCA TTCTTGAATG TGGCAGTGAA GATGTCAGAG TGGTCCGTCC TAAACCAACA 
CCCATGGACC CCATAGACAG AAGCTGCCTG AAGTCCTTCC TGTCCTTGGT CAACCTTCTA 
TCCTCTTCCC AATCGGTGTG GGAGCTGCTG GGTCGGCAGC CTTTAGCCAA CAAGAGCGAA 
TACACCCTAA GGGAAATGCC ATCCTCCATA CCTGATTTGA TTGACAGGCT GATTGCTGTG 
AATTCAGATG TGAAGATTCA TTCCCTGTTC CACTATGAGC AGTCTCACAC GTTTGGCCTG 
AGACTGCTCA GTGTGCTCTG CTGCAATCTG GACTCCTTTT TGCTGATGGA AAGCCAGTAT 
AACATCTGCT CCATGCTGCT GCAGAGTCAG AGAGAGAACA TCACTGACCT GGACACCAGC 
GAAGGGGAGT TCATCATAGA CAGCCTGTCG GTGGAGAGGA ACCATGTGCT GGTTCGTGTT 
AGTGTTGTCG GAGGTCCATC TGAGAGGAGA CTTCCTTCTC GAGCCCTGCA AGAGGGAGAA 
CATCCTTACC CCTGGCCTAT GTTTTCATGC TATCCTCTAC CTCGATGCTA CAGTTTGGAC 
CCACCAGAGA TCCAACATAG CTCACAGGAC TCTGAGATCA GCACATTTCT GGCTTCATCA 
AAAGACTCAA ACAATGATGA GAGCTGGATG GAGAACTGCC GCAGACAGTA CTGCAAAGTC 
GTGGCCTCGA AGCCCAACAT CCTAACTGGA AAAGTGCTGG CTGACCTTCT GGAGAATGTT 
GTAGCTCATC TATCCAGCTC TGCCACAGAG TGCTTCTTCA CTCCAGCTCG GTACAAAGGT 
CAGTGGAGAA GAGCAACACC TTCACTTTTT TTCTTTGACA ATTTAGTCAA ACAAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023120052.1
CDS	70..3249
Translation	MSQLSAEDEVELQSLLKQLLQSINEKIAGAPSTECAEEILLHLEETDKNFHNYEFVKYLRQYVENSLGTMVEEETKSLCRGDGQHAIGSGHDTLIHAVTRRTRDSAEYQQMMQTLKNTMIMVVESLINKIEEDQLRKEEMHREKQHSQSNSQYTDNCSDSDSSFNQSYAFIRQEQLQVLAEKLESSKPKEVRWEALQALCCAPPSDVLSCESWSSLRRNLCAALTEPDPDLSENVLQFFAKSFSSSPLNVTREIYTSLATSVDSSFLSQKLSFPSGSAVLDINRPEISRLLKQIRLMNDFQKEVTNFWIRHPEKYMEEVIESTFFLLSFHHEHGSAGTEKALEPIHLLALLDVKATWFKKWMHGYYSRTVVLRLLERKYKSLVVAALQQCIHYIESCEHFTNEVTDPQSEEQQNSGSVQRSVYSGKELEYSFFVHSLCVLGKLIIYTNGRKLFPIKMRKHKDPVSLTDLVVILIKIMYRHPQPLHSDTSHTDSLSPSSLVMEVLWMLCERTECAAECLYQTPVIETLLAPVIALLNGQQSQLKSPAATLTLIADVLARIANTDRGLALFLYEKNLVLAHSERTFAAHVIVQFTLRLLDKGLPSLSESDVSHTLCGAFIFVCRQIYNTCEGLQVLRPYGLHKAVAAAWKQTSSLSERVPTPVPGASAGTSTQELQHIHIWEETLLDSLLNFAATPKGLLLLQQTGAINECVSYMFSRFTKKLQVSRCEKFGYGVMVTQVAATAPGVVALHSSGFVRALVVELWSILECGSEDVRVVRPKPTPMDPIDRSCLKSFLSLVNLLSSSQSVWELLGRQPLANKSEYTLREMPSSIPDLIDRLIAVNSDVKIHSLFHYEQSHTFGLRLLSVLCCNLDSFLLMESQYNICSMLLQSQRENITDLDTSEGEFIIDSLSVERNHVLVRVSVVGGPSERRLPSRALQEGEHPYPWPMFSCYPLPRCYSLDPPEIQHSSQDSEISTFLASSKDSNNDESWMENCRRQYCKVVASKPNILTGKVLADLLENVVAHLSSSATECFFTPARYKGQWRRATPSLFFFDNLVKQK

Target ORF information:

RefSeq Version	XM_023264284.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris TBC1 domain family member 32 (tbc1d32), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023264284.1

ORF Insert Sequence:

ATGTCTCAGT TATCAGCAGA AGATGAGGTG GAGTTACAGT CCCTCCTCAA GCAGCTTCTG 
CAAAGTATCA ATGAAAAAAT TGCTGGTGCG CCGTCAACTG AATGTGCTGA AGAGATCCTA 
CTTCATCTGG AGGAGACTGA CAAGAACTTC CACAATTATG AGTTTGTGAA GTACCTGCGT 
CAGTATGTGG AGAACTCGCT GGGGACTATG GTGGAAGAGG AGACAAAGAG CTTATGCAGA 
GGAGATGGGC AACATGCAAT CGGTTCAGGA CATGACACCC TGATTCACGC TGTGACTCGA 
AGGACCAGAG ACTCAGCTGA GTATCAGCAG ATGATGCAGA CATTGAAAAA CACCATGATC 
ATGGTTGTGG AGTCTTTAAT CAACAAAATT GAGGAGGACC AGCTGAGGAA GGAGGAGATG 
CACCGGGAGA AGCAGCACAG TCAGTCCAAC AGCCAGTACA CTGATAACTG CTCTGACAGC 
GACTCCTCCT TCAACCAGAG TTATGCATTC ATCAGACAAG AGCAGCTGCA AGTTTTGGCT 
GAAAAACTTG AGTCCAGCAA GCCCAAAGAG GTGCGTTGGG AGGCCCTGCA GGCCCTCTGC 
TGTGCGCCTC CATCCGATGT GCTGAGCTGT GAGAGCTGGA GCAGCCTGCG CAGAAACCTC 
TGTGCGGCTC TGACTGAACC AGACCCCGAC CTCAGCGAGA ATGTACTGCA GTTCTTTGCT 
AAAAGCTTCT CGTCCTCTCC ACTGAATGTG ACACGAGAGA TCTACACCAG CTTGGCGACA 
AGTGTGGACT CAAGCTTTCT GTCTCAGAAG TTGAGCTTTC CAAGTGGCTC AGCTGTTCTC 
GACATCAACA GACCTGAGAT CAGTCGGCTT CTCAAACAGA TTCGATTGAT GAATGACTTT 
CAAAAGGAGG TGACTAACTT TTGGATCCGT CATCCAGAGA AATATATGGA GGAAGTTATA 
GAGAGCACGT TCTTCCTGTT ATCCTTCCAT CATGAACATG GCTCAGCAGG AACAGAGAAA 
GCCCTGGAAC CAATTCACTT ACTGGCCTTA CTGGATGTTA AAGCTACCTG GTTTAAAAAA 
TGGATGCATG GCTACTACAG CAGGACTGTG GTTCTCAGAC TCCTGGAGAG AAAATACAAG 
TCTTTGGTTG TAGCAGCTCT TCAGCAGTGT ATCCATTACA TTGAATCCTG TGAACATTTC 
ACAAATGAGG TAACTGATCC CCAGTCAGAG GAGCAGCAGA ACTCTGGTTC AGTGCAGAGG 
AGTGTTTATT CGGGAAAGGA GCTTGAATAT TCCTTCTTTG TCCACTCTCT GTGTGTCCTT 
GGGAAACTTA TCATCTACAC CAATGGAAGA AAGCTGTTTC CCATCAAAAT GAGGAAGCAC 
AAAGATCCAG TCAGCCTGAC AGACCTGGTT GTCATCCTTA TTAAGATCAT GTATCGGCAC 
CCCCAGCCTC TGCACAGTGA TACCTCTCAC ACAGACTCCC TGTCGCCCAG CAGTCTGGTG 
ATGGAGGTGC TGTGGATGCT ATGTGAACGG ACAGAGTGTG CTGCAGAGTG TCTCTACCAG 
ACTCCTGTCA TAGAGACGCT GCTGGCTCCG GTTATAGCTC TGCTCAATGG ACAGCAGTCC 
CAATTAAAGT CTCCTGCAGC AACACTGACT CTCATTGCTG ACGTTCTGGC TCGCATCGCA 
AACACTGACA GAGGATTAGC TCTCTTCTTA TATGAGAAGA ATCTCGTTCT AGCCCACAGT 
GAGAGAACCT TTGCTGCTCA TGTTATTGTG CAGTTCACCC TGAGGCTGCT GGATAAGGGG 
CTACCATCAC TAAGTGAATC AGATGTGTCC CATACATTAT GTGGAGCCTT TATCTTTGTG 
TGTCGGCAGA TATACAACAC CTGTGAGGGT CTGCAGGTTC TCCGACCCTA CGGGCTGCAC 
AAAGCTGTAG CGGCTGCATG GAAACAGACT AGTTCTTTGT CAGAAAGGGT TCCAACGCCA 
GTACCTGGAG CCAGTGCTGG AACATCTACC CAAGAGCTGC AGCACATTCA CATCTGGGAG 
GAGACATTGT TGGACAGTCT GCTGAACTTT GCAGCAACTC CTAAAGGCCT GCTGCTGCTC 
CAGCAGACAG GAGCCATTAA TGAGTGTGTC TCCTACATGT TCTCTCGCTT TACCAAAAAG 
CTACAGGTGA GCCGCTGTGA GAAGTTTGGA TATGGGGTGA TGGTGACACA GGTGGCAGCA 
ACTGCTCCAG GGGTCGTGGC TCTGCACAGC TCTGGTTTTG TCCGGGCACT GGTAGTAGAG 
CTGTGGTCCA TTCTTGAATG TGGCAGTGAA GATGTCAGAG TGGTCCGTCC TAAACCAACA 
CCCATGGACC CCATAGACAG AAGCTGCCTG AAGTCCTTCC TGTCCTTGGT CAACCTTCTA 
TCCTCTTCCC AATCGGTGTG GGAGCTGCTG GGTCGGCAGC CTTTAGCCAA CAAGAGCGAA 
TACACCCTAA GGGAAATGCC ATCCTCCATA CCTGATTTGA TTGACAGGCT GATTGCTGTG 
AATTCAGATG TGAAGATTCA TTCCCTGTTC CACTATGAGC AGTCTCACAC GTTTGGCCTG 
AGACTGCTCA GTGTGCTCTG CTGCAATCTG GACTCCTTTT TGCTGATGGA AAGCCAGTAT 
AACATCTGCT CCATGCTGCT GCAGAGTCAG AGAGAGAACA TCACTGACCT GGACACCAGC 
GAAGGGGAGT TCATCATAGA CAGCCTGTCG GTGGAGAGGA ACCATGTGCT GGTTCGTGTT 
AGTGTTGTCG GAGGTCCATC TGAGAGGAGA CTTCCTTCTC GAGCCCTGCA AGAGGGAGAA 
CATCCTTACC CCTGGCCTAT GTTTTCATGC TATCCTCTAC CTCGATGCTA CAGTTTGGAC 
CCACCAGAGA TCCAACATAG CTCACAGGAC TCTGAGATCA GCACATTTCT GGCTTCATCA 
AAAGACTCAA ACAATGATGA GAGCTGGATG GAGAACTGCC GCAGACAGTA CTGCAAAGTC 
GTGGCCTCGA AGCCCAACAT CCTAACTGGA AAAGTGCTGG CTGACCTTCT GGAGAATGTT 
GTAGCTCATC TATCCAGCTC TGCCACAGAG TGCTTCTTCA CTCCAGCTCG GTACAAAGGT 
CAGTGGAGAA GAGCAACACC TTCACTTTTT TTCTTTGACA ATTTAGTCAA ACAAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tbc1d32 ( XM_023264286.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023120054.1 Amphiprion ocellaris TBC1 domain family member 32 (tbc1d32), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAm103566
Clone ID Related Accession (Same CDS sequence)	XM_023264284.1 , XM_023264286.1
Accession Version	XM_023264286.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3180bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	protein broad-minded
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019376179.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGT TATCAGCAGA AGATGAGGTG GAGTTACAGT CCCTCCTCAA GCAGCTTCTG 
CAAAGTATCA ATGAAAAAAT TGCTGGTGCG CCGTCAACTG AATGTGCTGA AGAGATCCTA 
CTTCATCTGG AGGAGACTGA CAAGAACTTC CACAATTATG AGTTTGTGAA GTACCTGCGT 
CAGTATGTGG AGAACTCGCT GGGGACTATG GTGGAAGAGG AGACAAAGAG CTTATGCAGA 
GGAGATGGGC AACATGCAAT CGGTTCAGGA CATGACACCC TGATTCACGC TGTGACTCGA 
AGGACCAGAG ACTCAGCTGA GTATCAGCAG ATGATGCAGA CATTGAAAAA CACCATGATC 
ATGGTTGTGG AGTCTTTAAT CAACAAAATT GAGGAGGACC AGCTGAGGAA GGAGGAGATG 
CACCGGGAGA AGCAGCACAG TCAGTCCAAC AGCCAGTACA CTGATAACTG CTCTGACAGC 
GACTCCTCCT TCAACCAGAG TTATGCATTC ATCAGACAAG AGCAGCTGCA AGTTTTGGCT 
GAAAAACTTG AGTCCAGCAA GCCCAAAGAG GTGCGTTGGG AGGCCCTGCA GGCCCTCTGC 
TGTGCGCCTC CATCCGATGT GCTGAGCTGT GAGAGCTGGA GCAGCCTGCG CAGAAACCTC 
TGTGCGGCTC TGACTGAACC AGACCCCGAC CTCAGCGAGA ATGTACTGCA GTTCTTTGCT 
AAAAGCTTCT CGTCCTCTCC ACTGAATGTG ACACGAGAGA TCTACACCAG CTTGGCGACA 
AGTGTGGACT CAAGCTTTCT GTCTCAGAAG TTGAGCTTTC CAAGTGGCTC AGCTGTTCTC 
GACATCAACA GACCTGAGAT CAGTCGGCTT CTCAAACAGA TTCGATTGAT GAATGACTTT 
CAAAAGGAGG TGACTAACTT TTGGATCCGT CATCCAGAGA AATATATGGA GGAAGTTATA 
GAGAGCACGT TCTTCCTGTT ATCCTTCCAT CATGAACATG GCTCAGCAGG AACAGAGAAA 
GCCCTGGAAC CAATTCACTT ACTGGCCTTA CTGGATGTTA AAGCTACCTG GTTTAAAAAA 
TGGATGCATG GCTACTACAG CAGGACTGTG GTTCTCAGAC TCCTGGAGAG AAAATACAAG 
TCTTTGGTTG TAGCAGCTCT TCAGCAGTGT ATCCATTACA TTGAATCCTG TGAACATTTC 
ACAAATGAGG TAACTGATCC CCAGTCAGAG GAGCAGCAGA ACTCTGGTTC AGTGCAGAGG 
AGTGTTTATT CGGGAAAGGA GCTTGAATAT TCCTTCTTTG TCCACTCTCT GTGTGTCCTT 
GGGAAACTTA TCATCTACAC CAATGGAAGA AAGCTGTTTC CCATCAAAAT GAGGAAGCAC 
AAAGATCCAG TCAGCCTGAC AGACCTGGTT GTCATCCTTA TTAAGATCAT GTATCGGCAC 
CCCCAGCCTC TGCACAGTGA TACCTCTCAC ACAGACTCCC TGTCGCCCAG CAGTCTGGTG 
ATGGAGGTGC TGTGGATGCT ATGTGAACGG ACAGAGTGTG CTGCAGAGTG TCTCTACCAG 
ACTCCTGTCA TAGAGACGCT GCTGGCTCCG GTTATAGCTC TGCTCAATGG ACAGCAGTCC 
CAATTAAAGT CTCCTGCAGC AACACTGACT CTCATTGCTG ACGTTCTGGC TCGCATCGCA 
AACACTGACA GAGGATTAGC TCTCTTCTTA TATGAGAAGA ATCTCGTTCT AGCCCACAGT 
GAGAGAACCT TTGCTGCTCA TGTTATTGTG CAGTTCACCC TGAGGCTGCT GGATAAGGGG 
CTACCATCAC TAAGTGAATC AGATGTGTCC CATACATTAT GTGGAGCCTT TATCTTTGTG 
TGTCGGCAGA TATACAACAC CTGTGAGGGT CTGCAGGTTC TCCGACCCTA CGGGCTGCAC 
AAAGCTGTAG CGGCTGCATG GAAACAGACT AGTTCTTTGT CAGAAAGGGT TCCAACGCCA 
GTACCTGGAG CCAGTGCTGG AACATCTACC CAAGAGCTGC AGCACATTCA CATCTGGGAG 
GAGACATTGT TGGACAGTCT GCTGAACTTT GCAGCAACTC CTAAAGGCCT GCTGCTGCTC 
CAGCAGACAG GAGCCATTAA TGAGTGTGTC TCCTACATGT TCTCTCGCTT TACCAAAAAG 
CTACAGGTGA GCCGCTGTGA GAAGTTTGGA TATGGGGTGA TGGTGACACA GGTGGCAGCA 
ACTGCTCCAG GGGTCGTGGC TCTGCACAGC TCTGGTTTTG TCCGGGCACT GGTAGTAGAG 
CTGTGGTCCA TTCTTGAATG TGGCAGTGAA GATGTCAGAG TGGTCCGTCC TAAACCAACA 
CCCATGGACC CCATAGACAG AAGCTGCCTG AAGTCCTTCC TGTCCTTGGT CAACCTTCTA 
TCCTCTTCCC AATCGGTGTG GGAGCTGCTG GGTCGGCAGC CTTTAGCCAA CAAGAGCGAA 
TACACCCTAA GGGAAATGCC ATCCTCCATA CCTGATTTGA TTGACAGGCT GATTGCTGTG 
AATTCAGATG TGAAGATTCA TTCCCTGTTC CACTATGAGC AGTCTCACAC GTTTGGCCTG 
AGACTGCTCA GTGTGCTCTG CTGCAATCTG GACTCCTTTT TGCTGATGGA AAGCCAGTAT 
AACATCTGCT CCATGCTGCT GCAGAGTCAG AGAGAGAACA TCACTGACCT GGACACCAGC 
GAAGGGGAGT TCATCATAGA CAGCCTGTCG GTGGAGAGGA ACCATGTGCT GGTTCGTGTT 
AGTGTTGTCG GAGGTCCATC TGAGAGGAGA CTTCCTTCTC GAGCCCTGCA AGAGGGAGAA 
CATCCTTACC CCTGGCCTAT GTTTTCATGC TATCCTCTAC CTCGATGCTA CAGTTTGGAC 
CCACCAGAGA TCCAACATAG CTCACAGGAC TCTGAGATCA GCACATTTCT GGCTTCATCA 
AAAGACTCAA ACAATGATGA GAGCTGGATG GAGAACTGCC GCAGACAGTA CTGCAAAGTC 
GTGGCCTCGA AGCCCAACAT CCTAACTGGA AAAGTGCTGG CTGACCTTCT GGAGAATGTT 
GTAGCTCATC TATCCAGCTC TGCCACAGAG TGCTTCTTCA CTCCAGCTCG GTACAAAGGT 
CAGTGGAGAA GAGCAACACC TTCACTTTTT TTCTTTGACA ATTTAGTCAA ACAAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023120054.1
CDS	60..3239
Translation	MSQLSAEDEVELQSLLKQLLQSINEKIAGAPSTECAEEILLHLEETDKNFHNYEFVKYLRQYVENSLGTMVEEETKSLCRGDGQHAIGSGHDTLIHAVTRRTRDSAEYQQMMQTLKNTMIMVVESLINKIEEDQLRKEEMHREKQHSQSNSQYTDNCSDSDSSFNQSYAFIRQEQLQVLAEKLESSKPKEVRWEALQALCCAPPSDVLSCESWSSLRRNLCAALTEPDPDLSENVLQFFAKSFSSSPLNVTREIYTSLATSVDSSFLSQKLSFPSGSAVLDINRPEISRLLKQIRLMNDFQKEVTNFWIRHPEKYMEEVIESTFFLLSFHHEHGSAGTEKALEPIHLLALLDVKATWFKKWMHGYYSRTVVLRLLERKYKSLVVAALQQCIHYIESCEHFTNEVTDPQSEEQQNSGSVQRSVYSGKELEYSFFVHSLCVLGKLIIYTNGRKLFPIKMRKHKDPVSLTDLVVILIKIMYRHPQPLHSDTSHTDSLSPSSLVMEVLWMLCERTECAAECLYQTPVIETLLAPVIALLNGQQSQLKSPAATLTLIADVLARIANTDRGLALFLYEKNLVLAHSERTFAAHVIVQFTLRLLDKGLPSLSESDVSHTLCGAFIFVCRQIYNTCEGLQVLRPYGLHKAVAAAWKQTSSLSERVPTPVPGASAGTSTQELQHIHIWEETLLDSLLNFAATPKGLLLLQQTGAINECVSYMFSRFTKKLQVSRCEKFGYGVMVTQVAATAPGVVALHSSGFVRALVVELWSILECGSEDVRVVRPKPTPMDPIDRSCLKSFLSLVNLLSSSQSVWELLGRQPLANKSEYTLREMPSSIPDLIDRLIAVNSDVKIHSLFHYEQSHTFGLRLLSVLCCNLDSFLLMESQYNICSMLLQSQRENITDLDTSEGEFIIDSLSVERNHVLVRVSVVGGPSERRLPSRALQEGEHPYPWPMFSCYPLPRCYSLDPPEIQHSSQDSEISTFLASSKDSNNDESWMENCRRQYCKVVASKPNILTGKVLADLLENVVAHLSSSATECFFTPARYKGQWRRATPSLFFFDNLVKQK

Target ORF information:

RefSeq Version	XM_023264286.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris TBC1 domain family member 32 (tbc1d32), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023264286.1

ORF Insert Sequence:

ATGTCTCAGT TATCAGCAGA AGATGAGGTG GAGTTACAGT CCCTCCTCAA GCAGCTTCTG 
CAAAGTATCA ATGAAAAAAT TGCTGGTGCG CCGTCAACTG AATGTGCTGA AGAGATCCTA 
CTTCATCTGG AGGAGACTGA CAAGAACTTC CACAATTATG AGTTTGTGAA GTACCTGCGT 
CAGTATGTGG AGAACTCGCT GGGGACTATG GTGGAAGAGG AGACAAAGAG CTTATGCAGA 
GGAGATGGGC AACATGCAAT CGGTTCAGGA CATGACACCC TGATTCACGC TGTGACTCGA 
AGGACCAGAG ACTCAGCTGA GTATCAGCAG ATGATGCAGA CATTGAAAAA CACCATGATC 
ATGGTTGTGG AGTCTTTAAT CAACAAAATT GAGGAGGACC AGCTGAGGAA GGAGGAGATG 
CACCGGGAGA AGCAGCACAG TCAGTCCAAC AGCCAGTACA CTGATAACTG CTCTGACAGC 
GACTCCTCCT TCAACCAGAG TTATGCATTC ATCAGACAAG AGCAGCTGCA AGTTTTGGCT 
GAAAAACTTG AGTCCAGCAA GCCCAAAGAG GTGCGTTGGG AGGCCCTGCA GGCCCTCTGC 
TGTGCGCCTC CATCCGATGT GCTGAGCTGT GAGAGCTGGA GCAGCCTGCG CAGAAACCTC 
TGTGCGGCTC TGACTGAACC AGACCCCGAC CTCAGCGAGA ATGTACTGCA GTTCTTTGCT 
AAAAGCTTCT CGTCCTCTCC ACTGAATGTG ACACGAGAGA TCTACACCAG CTTGGCGACA 
AGTGTGGACT CAAGCTTTCT GTCTCAGAAG TTGAGCTTTC CAAGTGGCTC AGCTGTTCTC 
GACATCAACA GACCTGAGAT CAGTCGGCTT CTCAAACAGA TTCGATTGAT GAATGACTTT 
CAAAAGGAGG TGACTAACTT TTGGATCCGT CATCCAGAGA AATATATGGA GGAAGTTATA 
GAGAGCACGT TCTTCCTGTT ATCCTTCCAT CATGAACATG GCTCAGCAGG AACAGAGAAA 
GCCCTGGAAC CAATTCACTT ACTGGCCTTA CTGGATGTTA AAGCTACCTG GTTTAAAAAA 
TGGATGCATG GCTACTACAG CAGGACTGTG GTTCTCAGAC TCCTGGAGAG AAAATACAAG 
TCTTTGGTTG TAGCAGCTCT TCAGCAGTGT ATCCATTACA TTGAATCCTG TGAACATTTC 
ACAAATGAGG TAACTGATCC CCAGTCAGAG GAGCAGCAGA ACTCTGGTTC AGTGCAGAGG 
AGTGTTTATT CGGGAAAGGA GCTTGAATAT TCCTTCTTTG TCCACTCTCT GTGTGTCCTT 
GGGAAACTTA TCATCTACAC CAATGGAAGA AAGCTGTTTC CCATCAAAAT GAGGAAGCAC 
AAAGATCCAG TCAGCCTGAC AGACCTGGTT GTCATCCTTA TTAAGATCAT GTATCGGCAC 
CCCCAGCCTC TGCACAGTGA TACCTCTCAC ACAGACTCCC TGTCGCCCAG CAGTCTGGTG 
ATGGAGGTGC TGTGGATGCT ATGTGAACGG ACAGAGTGTG CTGCAGAGTG TCTCTACCAG 
ACTCCTGTCA TAGAGACGCT GCTGGCTCCG GTTATAGCTC TGCTCAATGG ACAGCAGTCC 
CAATTAAAGT CTCCTGCAGC AACACTGACT CTCATTGCTG ACGTTCTGGC TCGCATCGCA 
AACACTGACA GAGGATTAGC TCTCTTCTTA TATGAGAAGA ATCTCGTTCT AGCCCACAGT 
GAGAGAACCT TTGCTGCTCA TGTTATTGTG CAGTTCACCC TGAGGCTGCT GGATAAGGGG 
CTACCATCAC TAAGTGAATC AGATGTGTCC CATACATTAT GTGGAGCCTT TATCTTTGTG 
TGTCGGCAGA TATACAACAC CTGTGAGGGT CTGCAGGTTC TCCGACCCTA CGGGCTGCAC 
AAAGCTGTAG CGGCTGCATG GAAACAGACT AGTTCTTTGT CAGAAAGGGT TCCAACGCCA 
GTACCTGGAG CCAGTGCTGG AACATCTACC CAAGAGCTGC AGCACATTCA CATCTGGGAG 
GAGACATTGT TGGACAGTCT GCTGAACTTT GCAGCAACTC CTAAAGGCCT GCTGCTGCTC 
CAGCAGACAG GAGCCATTAA TGAGTGTGTC TCCTACATGT TCTCTCGCTT TACCAAAAAG 
CTACAGGTGA GCCGCTGTGA GAAGTTTGGA TATGGGGTGA TGGTGACACA GGTGGCAGCA 
ACTGCTCCAG GGGTCGTGGC TCTGCACAGC TCTGGTTTTG TCCGGGCACT GGTAGTAGAG 
CTGTGGTCCA TTCTTGAATG TGGCAGTGAA GATGTCAGAG TGGTCCGTCC TAAACCAACA 
CCCATGGACC CCATAGACAG AAGCTGCCTG AAGTCCTTCC TGTCCTTGGT CAACCTTCTA 
TCCTCTTCCC AATCGGTGTG GGAGCTGCTG GGTCGGCAGC CTTTAGCCAA CAAGAGCGAA 
TACACCCTAA GGGAAATGCC ATCCTCCATA CCTGATTTGA TTGACAGGCT GATTGCTGTG 
AATTCAGATG TGAAGATTCA TTCCCTGTTC CACTATGAGC AGTCTCACAC GTTTGGCCTG 
AGACTGCTCA GTGTGCTCTG CTGCAATCTG GACTCCTTTT TGCTGATGGA AAGCCAGTAT 
AACATCTGCT CCATGCTGCT GCAGAGTCAG AGAGAGAACA TCACTGACCT GGACACCAGC 
GAAGGGGAGT TCATCATAGA CAGCCTGTCG GTGGAGAGGA ACCATGTGCT GGTTCGTGTT 
AGTGTTGTCG GAGGTCCATC TGAGAGGAGA CTTCCTTCTC GAGCCCTGCA AGAGGGAGAA 
CATCCTTACC CCTGGCCTAT GTTTTCATGC TATCCTCTAC CTCGATGCTA CAGTTTGGAC 
CCACCAGAGA TCCAACATAG CTCACAGGAC TCTGAGATCA GCACATTTCT GGCTTCATCA 
AAAGACTCAA ACAATGATGA GAGCTGGATG GAGAACTGCC GCAGACAGTA CTGCAAAGTC 
GTGGCCTCGA AGCCCAACAT CCTAACTGGA AAAGTGCTGG CTGACCTTCT GGAGAATGTT 
GTAGCTCATC TATCCAGCTC TGCCACAGAG TGCTTCTTCA CTCCAGCTCG GTACAAAGGT 
CAGTGGAGAA GAGCAACACC TTCACTTTTT TTCTTTGACA ATTTAGTCAA ACAAAAATAA