LOC105928766 cDNA ORF clone, Fundulus heteroclitus(mummichog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105928766
Entrez Gene ID	105928766
Full Name	transmembrane protein 132D-like
Gene Type	protein-coding
Organism	Fundulus heteroclitus(mummichog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012866229.1	XP_012721683.1	transmembrane protein 132D-like
XM_012866229.2	XP_012721683.1	transmembrane protein 132D-like

Pathways from BioSystems
Gene Ontology

The following LOC105928766 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105928766 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFu112416	XM_012866229.2 Latest version!	Fundulus heteroclitus transmembrane protein 132D-like (LOC105928766), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
OFu112416	XM_012866229.1	Fundulus heteroclitus transmembrane protein 132D-like (LOC105928766), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105928766 ( XM_012866229.1 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_012721683.1 Fundulus heteroclitus transmembrane protein 132D-like (LOC105928766), mRNA.

Price & Availability↑

CloneID	OFu112416
Clone ID Related Accession (Same CDS sequence)	XM_012866229.1 , XM_012866229.2
Accession Version	XM_012866229.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-14
Organism	Fundulus heteroclitus(Mummichog)
Product	transmembrane protein 132D-like
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012234300.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTTA CCTGGCAAAG CTGGATGGTC CCGTTGTTAG TGCTTGCAAC AGTACCAGCA 
GTACTTACAC AAGATCTAAA CAGCAAGGAA GTCACTGACA TTAAGAGTCC ATTGTCTTTC 
CCTGTCTCCC TGCCGGTCAA CTATGAGGTA CGAGATGCCG ACTACCTCTT CCTCAAGGAG 
GCTGGACAGG ACTTCATGAG GAACTCCAGC ATGCAGAGTC ACACCCAGCC CTTTGTTTTA 
TTAAGAGCCA GCCGCCAGCC GGTTGTCAAT GCCAGCTATG GTCCCGTGTC CACAGAGAGA 
CCCGTGCCTC TCGATCTGGT TCAGGCTGTG CAGCTCTTTA ACGCTCCAAA TGTCTTTACA 
TTTAACTGGA AAATCCAGGC CTTTGTGCTA ACGCCTCGGG TTTTCTCCTC CAAACCCAAA 
GTTCGGGTTC TCTTCTACGT CGCAGGCAGG GATTGGGACA GAGGCGAGGG AGCCGTGGAT 
GAGCTACCTT GTGTGACGGT TTTCGCTTTC TGGCAAACGC AGGAGGTGAG GGGTTCTTGT 
GCAATCGGAG GCGAGAGGGG AACTTGCATG GCTGAGCTGT CGCCTGAGCC TGGCTGGTTC 
GCTGCAGGGT CAGAGGGCAT CAGCAGGGAG AGGCAGGATC CGTCTGCTGG GAACCCAGTG 
GAGCTGTACT ACCAGGCCCA GCCCAGGGTC AATGGGAAGT GTTTTTCTGT GAACGGAAGC 
CGCTGGAGCA GCTCGGCGCA GCCGGCCGAG TATGTTCCTG TGACTCCTAT GCAGAGGATC 
GGAAGCGTGC AACTTCTGCA GATTCCCAAA GGGATGGCGA CGCTCTCTCG CCTCAAGCTA 
GGGAACGCGA TAGTCATTCG GACGTCATCC AAGCCTCTGA AAAAGACCGA CATCGCCACC 
TTCTACATAC TGATGGCCGG CTCCGCTCAG CTGACTAACT TCACTCTCAG AGCATCAGTA 
AAGAAAGGTG TGACCTTTCG AACAGCGACA CCCAGCAACT CTTTACTGTG GGACATCACG 
CTCGATATGG GAGCAGATGG TGCCATTGCT GTTATTTGTC AGAGAAAGAC CCCCATACCT 
GGGAAAAGGC TTGAAAGCAG TCTGCTGGAG GTTCTTCAAA TGGATTTTGA GGTAGAAGAA 
CTGAGCAGTC CCGTTGACAG CCAGGTGATC ATATGGAAGC TGGAGCTACC ATCTGTGCCC 
AAAACTATTG CCAAGACTGA AGGAGCCATG AGAATTTATA CAACTCAGAG AGACTTTGTT 
GGCCTTGCTC CTCTTGTGAT GGACACCGAG ATCCTGAACA CTGCTGTTCT GACTGGAAAG 
AAAGTTATGA TGCCTGTGAG GACGGTAGCT GTGGAGGAAG ATGGAGTGGT CACAGATGTT 
TCTGACTACA CAGACTGCAG CTCCACTGAT GAAGACGTTC TCAAGGTGTC AGACAGATGT 
GACTATGTTT TTGTGAATGG CAAGGAGACC AAAGGCAAGG TGAAGATGAT AGTAAACTTC 
ACCTACAGCT ACCTGAGTGC TCAGCTCGAA CTAAATGTGT GGATCCCTCG GCTCCCTCTG 
CAGATTGAGT TGTCAGACAC AGAACTGAGC CAGATTAAAA GCTGGAGAGT TCCTATTCTG 
ACCTCGAAAA GATCTAGCTG GAACAATGAT GAGGATGAAC GAAAGGGCAA AGGCTGCATG 
CTCCAGCTTC AACACGCACT TGTGCGGGTT CTTACCCATT TTGTGGCTGA ACAGGAAGAC 
CCCCGCGACC CTACAGCCTA TTTCCTGGGC TCTGATTGGC AGGTGGATAT TACAAGGTTG 
GTACGATACT TCATGAAGGT CGAGGACACT CGAGTTGCAA GACTTCAGGA AGGAAGAGTG 
CTGTCAGGCC GAGACTTTGG GACGACAAAC ATCCAGGTTT TTTCTCCACT GTCTGATGTC 
ATCTTGGCAA AAACAACAGT CAAAGTTGTG GATGACAAAG TGAGCATCAC CGAATTAGGT 
GTTCAAGTGG TTACTGGACT ATCCATGACG TTACAACGTA GTTCAGGAAG TAGCAGGGCC 
ATCTTGGCTA CCACAACCAC ACAAGAGGTT CTACAAAGCC CCAAACAGGA AGCTTTGGTC 
AGTCCGTGGG TCCAGTTCAG TGATGGCAAC CAGACACCTC TTGACATTTA TGATCCAGCA 
AACTACCGAA TGACAGTGAC GTCTCTAGAT CAGGGGGTGG TGTCAGTGCA GGGTAGACCT 
CCTGTCGTAG TGGCGGAGGG TGAGGGTGAA GGAGTCTTAG TCAGGGTAGA GATGTCCATC 
TGTGAGTCCT GCCAAAAGTC TAAACGCAAA AGTACTGTGG CTGTGGGCAA TGGCAGCCTT 
AAGGTCAAGT TTCAGGCCAA CACACGGCGG CCAGGTGGTA GTACCCGCAG CGCGGACCGA 
AGCCCTGCTG GCAACAAAGA ACGCAACAAT GACTATGGGA ATGATGGGGA AGAGATGGAC 
AGTGAGAAGA AGCAAAGGAT GCCATCTCAG TTTCTTCCTT CAAGTATTCC AAACCCCAAC 
AAAGAGGAGA GTGTCATTCA GAAGATCCCA ACAACGATCA AATCTACAGA GAGAACCATG 
ATAACCAGCG GCGGTCTCGG AGGAGCGGGC AAGACAAGAA GACCAGAAAA TGTTGGCAGT 
CCGACCAGCG TAATGAATTT CAGCCTAATA AGCAGCCCCA GAGACAGCAG CAAATCATAT 
AGCTCAGACA ACATGATTGG GGAGGATATG AGCAGTGTTA GTTTTACCAG CACTGTGAAA 
GCTCCTGGGA ATCTAGTAAA CTACAACAAC TTCCCCACCA AGGTAGAGAT CCCTGGACCA 
GAAATGCCAG AGGTAGAAAG TGGTGAAGAA GCTTTGGCCA ACAGGCCTCT CACAGACTTG 
GAGATTGGCA TGTATGCCTT GTTGGGTGTT TTTTGCCTTG CCATCTTGGT TTTTCTTGTG 
AACTGTATTT CATATGTTGT AAAATTCAGG CACAAGAAGC CTCCCTCTCA TGGCCAGGAG 
CCAACAGGGC ACAGGCACGA CTGGGTCTGG CTGGGCACAG ACGCTGAGCT GGTGATGACC 
GTGCCATGCA GTCCGGTCCA GCAAGACTCA AATACAGCAA CCACTGTCAT CGACATTGGG 
CCCGACAAGA CTGCCTCTCT TTCCAGGAGG CCCAGTTGCC TGGCCTCTGT ATCGGACTCT 
CCTCTCAGCG GTGCCAGCTC TCACAGGACC AAACCCATAC AAAGCGAGTC CCTCCACTCG 
CCCACCAGCA AGAGAAAGAG AGTTCAGTTC ACCACGTTCT CCACACTGGA GCGCCAGCAT 
TCGCCACACC TGTCACCCAA GGAAAATGGT CATGGTATCC ACTGGGTTGG GAAGGAAGAG 
TGCAATGGAG AGGAACCTCA AGTGCCCATC ATAAAGCCTG GGGACCAGTT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012721683.1
CDS	326..3739
Translation	MSVTWQSWMVPLLVLATVPAVLTQDLNSKEVTDIKSPLSFPVSLPVNYEVRDADYLFLKEAGQDFMRNSSMQSHTQPFVLLRASRQPVVNASYGPVSTERPVPLDLVQAVQLFNAPNVFTFNWKIQAFVLTPRVFSSKPKVRVLFYVAGRDWDRGEGAVDELPCVTVFAFWQTQEVRGSCAIGGERGTCMAELSPEPGWFAAGSEGISRERQDPSAGNPVELYYQAQPRVNGKCFSVNGSRWSSSAQPAEYVPVTPMQRIGSVQLLQIPKGMATLSRLKLGNAIVIRTSSKPLKKTDIATFYILMAGSAQLTNFTLRASVKKGVTFRTATPSNSLLWDITLDMGADGAIAVICQRKTPIPGKRLESSLLEVLQMDFEVEELSSPVDSQVIIWKLELPSVPKTIAKTEGAMRIYTTQRDFVGLAPLVMDTEILNTAVLTGKKVMMPVRTVAVEEDGVVTDVSDYTDCSSTDEDVLKVSDRCDYVFVNGKETKGKVKMIVNFTYSYLSAQLELNVWIPRLPLQIELSDTELSQIKSWRVPILTSKRSSWNNDEDERKGKGCMLQLQHALVRVLTHFVAEQEDPRDPTAYFLGSDWQVDITRLVRYFMKVEDTRVARLQEGRVLSGRDFGTTNIQVFSPLSDVILAKTTVKVVDDKVSITELGVQVVTGLSMTLQRSSGSSRAILATTTTQEVLQSPKQEALVSPWVQFSDGNQTPLDIYDPANYRMTVTSLDQGVVSVQGRPPVVVAEGEGEGVLVRVEMSICESCQKSKRKSTVAVGNGSLKVKFQANTRRPGGSTRSADRSPAGNKERNNDYGNDGEEMDSEKKQRMPSQFLPSSIPNPNKEESVIQKIPTTIKSTERTMITSGGLGGAGKTRRPENVGSPTSVMNFSLISSPRDSSKSYSSDNMIGEDMSSVSFTSTVKAPGNLVNYNNFPTKVEIPGPEMPEVESGEEALANRPLTDLEIGMYALLGVFCLAILVFLVNCISYVVKFRHKKPPSHGQEPTGHRHDWVWLGTDAELVMTVPCSPVQQDSNTATTVIDIGPDKTASLSRRPSCLASVSDSPLSGASSHRTKPIQSESLHSPTSKRKRVQFTTFSTLERQHSPHLSPKENGHGIHWVGKEECNGEEPQVPIIKPGDQL

Target ORF information:

RefSeq Version	XM_012866229.1
Organism	Fundulus heteroclitus(Mummichog)
Definition	Fundulus heteroclitus transmembrane protein 132D-like (LOC105928766), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012866229.1

ORF Insert Sequence:

ATGAGCGTTA CCTGGCAAAG CTGGATGGTC CCGTTGTTAG TGCTTGCAAC AGTACCAGCA 
GTACTTACAC AAGATCTAAA CAGCAAGGAA GTCACTGACA TTAAGAGTCC ATTGTCTTTC 
CCTGTCTCCC TGCCGGTCAA CTATGAGGTA CGAGATGCCG ACTACCTCTT CCTCAAGGAG 
GCTGGACAGG ACTTCATGAG GAACTCCAGC ATGCAGAGTC ACACCCAGCC CTTTGTTTTA 
TTAAGAGCCA GCCGCCAGCC GGTTGTCAAT GCCAGCTATG GTCCCGTGTC CACAGAGAGA 
CCCGTGCCTC TCGATCTGGT TCAGGCTGTG CAGCTCTTTA ACGCTCCAAA TGTCTTTACA 
TTTAACTGGA AAATCCAGGC CTTTGTGCTA ACGCCTCGGG TTTTCTCCTC CAAACCCAAA 
GTTCGGGTTC TCTTCTACGT CGCAGGCAGG GATTGGGACA GAGGCGAGGG AGCCGTGGAT 
GAGCTACCTT GTGTGACGGT TTTCGCTTTC TGGCAAACGC AGGAGGTGAG GGGTTCTTGT 
GCAATCGGAG GCGAGAGGGG AACTTGCATG GCTGAGCTGT CGCCTGAGCC TGGCTGGTTC 
GCTGCAGGGT CAGAGGGCAT CAGCAGGGAG AGGCAGGATC CGTCTGCTGG GAACCCAGTG 
GAGCTGTACT ACCAGGCCCA GCCCAGGGTC AATGGGAAGT GTTTTTCTGT GAACGGAAGC 
CGCTGGAGCA GCTCGGCGCA GCCGGCCGAG TATGTTCCTG TGACTCCTAT GCAGAGGATC 
GGAAGCGTGC AACTTCTGCA GATTCCCAAA GGGATGGCGA CGCTCTCTCG CCTCAAGCTA 
GGGAACGCGA TAGTCATTCG GACGTCATCC AAGCCTCTGA AAAAGACCGA CATCGCCACC 
TTCTACATAC TGATGGCCGG CTCCGCTCAG CTGACTAACT TCACTCTCAG AGCATCAGTA 
AAGAAAGGTG TGACCTTTCG AACAGCGACA CCCAGCAACT CTTTACTGTG GGACATCACG 
CTCGATATGG GAGCAGATGG TGCCATTGCT GTTATTTGTC AGAGAAAGAC CCCCATACCT 
GGGAAAAGGC TTGAAAGCAG TCTGCTGGAG GTTCTTCAAA TGGATTTTGA GGTAGAAGAA 
CTGAGCAGTC CCGTTGACAG CCAGGTGATC ATATGGAAGC TGGAGCTACC ATCTGTGCCC 
AAAACTATTG CCAAGACTGA AGGAGCCATG AGAATTTATA CAACTCAGAG AGACTTTGTT 
GGCCTTGCTC CTCTTGTGAT GGACACCGAG ATCCTGAACA CTGCTGTTCT GACTGGAAAG 
AAAGTTATGA TGCCTGTGAG GACGGTAGCT GTGGAGGAAG ATGGAGTGGT CACAGATGTT 
TCTGACTACA CAGACTGCAG CTCCACTGAT GAAGACGTTC TCAAGGTGTC AGACAGATGT 
GACTATGTTT TTGTGAATGG CAAGGAGACC AAAGGCAAGG TGAAGATGAT AGTAAACTTC 
ACCTACAGCT ACCTGAGTGC TCAGCTCGAA CTAAATGTGT GGATCCCTCG GCTCCCTCTG 
CAGATTGAGT TGTCAGACAC AGAACTGAGC CAGATTAAAA GCTGGAGAGT TCCTATTCTG 
ACCTCGAAAA GATCTAGCTG GAACAATGAT GAGGATGAAC GAAAGGGCAA AGGCTGCATG 
CTCCAGCTTC AACACGCACT TGTGCGGGTT CTTACCCATT TTGTGGCTGA ACAGGAAGAC 
CCCCGCGACC CTACAGCCTA TTTCCTGGGC TCTGATTGGC AGGTGGATAT TACAAGGTTG 
GTACGATACT TCATGAAGGT CGAGGACACT CGAGTTGCAA GACTTCAGGA AGGAAGAGTG 
CTGTCAGGCC GAGACTTTGG GACGACAAAC ATCCAGGTTT TTTCTCCACT GTCTGATGTC 
ATCTTGGCAA AAACAACAGT CAAAGTTGTG GATGACAAAG TGAGCATCAC CGAATTAGGT 
GTTCAAGTGG TTACTGGACT ATCCATGACG TTACAACGTA GTTCAGGAAG TAGCAGGGCC 
ATCTTGGCTA CCACAACCAC ACAAGAGGTT CTACAAAGCC CCAAACAGGA AGCTTTGGTC 
AGTCCGTGGG TCCAGTTCAG TGATGGCAAC CAGACACCTC TTGACATTTA TGATCCAGCA 
AACTACCGAA TGACAGTGAC GTCTCTAGAT CAGGGGGTGG TGTCAGTGCA GGGTAGACCT 
CCTGTCGTAG TGGCGGAGGG TGAGGGTGAA GGAGTCTTAG TCAGGGTAGA GATGTCCATC 
TGTGAGTCCT GCCAAAAGTC TAAACGCAAA AGTACTGTGG CTGTGGGCAA TGGCAGCCTT 
AAGGTCAAGT TTCAGGCCAA CACACGGCGG CCAGGTGGTA GTACCCGCAG CGCGGACCGA 
AGCCCTGCTG GCAACAAAGA ACGCAACAAT GACTATGGGA ATGATGGGGA AGAGATGGAC 
AGTGAGAAGA AGCAAAGGAT GCCATCTCAG TTTCTTCCTT CAAGTATTCC AAACCCCAAC 
AAAGAGGAGA GTGTCATTCA GAAGATCCCA ACAACGATCA AATCTACAGA GAGAACCATG 
ATAACCAGCG GCGGTCTCGG AGGAGCGGGC AAGACAAGAA GACCAGAAAA TGTTGGCAGT 
CCGACCAGCG TAATGAATTT CAGCCTAATA AGCAGCCCCA GAGACAGCAG CAAATCATAT 
AGCTCAGACA ACATGATTGG GGAGGATATG AGCAGTGTTA GTTTTACCAG CACTGTGAAA 
GCTCCTGGGA ATCTAGTAAA CTACAACAAC TTCCCCACCA AGGTAGAGAT CCCTGGACCA 
GAAATGCCAG AGGTAGAAAG TGGTGAAGAA GCTTTGGCCA ACAGGCCTCT CACAGACTTG 
GAGATTGGCA TGTATGCCTT GTTGGGTGTT TTTTGCCTTG CCATCTTGGT TTTTCTTGTG 
AACTGTATTT CATATGTTGT AAAATTCAGG CACAAGAAGC CTCCCTCTCA TGGCCAGGAG 
CCAACAGGGC ACAGGCACGA CTGGGTCTGG CTGGGCACAG ACGCTGAGCT GGTGATGACC 
GTGCCATGCA GTCCGGTCCA GCAAGACTCA AATACAGCAA CCACTGTCAT CGACATTGGG 
CCCGACAAGA CTGCCTCTCT TTCCAGGAGG CCCAGTTGCC TGGCCTCTGT ATCGGACTCT 
CCTCTCAGCG GTGCCAGCTC TCACAGGACC AAACCCATAC AAAGCGAGTC CCTCCACTCG 
CCCACCAGCA AGAGAAAGAG AGTTCAGTTC ACCACGTTCT CCACACTGGA GCGCCAGCAT 
TCGCCACACC TGTCACCCAA GGAAAATGGT CATGGTATCC ACTGGGTTGG GAAGGAAGAG 
TGCAATGGAG AGGAACCTCA AGTGCCCATC ATAAAGCCTG GGGACCAGTT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105928766 ( XM_012866229.2 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_012721683.1 Fundulus heteroclitus transmembrane protein 132D-like (LOC105928766), mRNA.

Price & Availability↑

CloneID	OFu112416
Clone ID Related Accession (Same CDS sequence)	XM_012866229.1 , XM_012866229.2
Accession Version	XM_012866229.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-31
Organism	Fundulus heteroclitus(mummichog)
Product	transmembrane protein 132D-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012234300.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 1, 2017 this sequence version replaced XM_012866229.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTTA CCTGGCAAAG CTGGATGGTC CCGTTGTTAG TGCTTGCAAC AGTACCAGCA 
GTACTTACAC AAGATCTAAA CAGCAAGGAA GTCACTGACA TTAAGAGTCC ATTGTCTTTC 
CCTGTCTCCC TGCCGGTCAA CTATGAGGTA CGAGATGCCG ACTACCTCTT CCTCAAGGAG 
GCTGGACAGG ACTTCATGAG GAACTCCAGC ATGCAGAGTC ACACCCAGCC CTTTGTTTTA 
TTAAGAGCCA GCCGCCAGCC GGTTGTCAAT GCCAGCTATG GTCCCGTGTC CACAGAGAGA 
CCCGTGCCTC TCGATCTGGT TCAGGCTGTG CAGCTCTTTA ACGCTCCAAA TGTCTTTACA 
TTTAACTGGA AAATCCAGGC CTTTGTGCTA ACGCCTCGGG TTTTCTCCTC CAAACCCAAA 
GTTCGGGTTC TCTTCTACGT CGCAGGCAGG GATTGGGACA GAGGCGAGGG AGCCGTGGAT 
GAGCTACCTT GTGTGACGGT TTTCGCTTTC TGGCAAACGC AGGAGGTGAG GGGTTCTTGT 
GCAATCGGAG GCGAGAGGGG AACTTGCATG GCTGAGCTGT CGCCTGAGCC TGGCTGGTTC 
GCTGCAGGGT CAGAGGGCAT CAGCAGGGAG AGGCAGGATC CGTCTGCTGG GAACCCAGTG 
GAGCTGTACT ACCAGGCCCA GCCCAGGGTC AATGGGAAGT GTTTTTCTGT GAACGGAAGC 
CGCTGGAGCA GCTCGGCGCA GCCGGCCGAG TATGTTCCTG TGACTCCTAT GCAGAGGATC 
GGAAGCGTGC AACTTCTGCA GATTCCCAAA GGGATGGCGA CGCTCTCTCG CCTCAAGCTA 
GGGAACGCGA TAGTCATTCG GACGTCATCC AAGCCTCTGA AAAAGACCGA CATCGCCACC 
TTCTACATAC TGATGGCCGG CTCCGCTCAG CTGACTAACT TCACTCTCAG AGCATCAGTA 
AAGAAAGGTG TGACCTTTCG AACAGCGACA CCCAGCAACT CTTTACTGTG GGACATCACG 
CTCGATATGG GAGCAGATGG TGCCATTGCT GTTATTTGTC AGAGAAAGAC CCCCATACCT 
GGGAAAAGGC TTGAAAGCAG TCTGCTGGAG GTTCTTCAAA TGGATTTTGA GGTAGAAGAA 
CTGAGCAGTC CCGTTGACAG CCAGGTGATC ATATGGAAGC TGGAGCTACC ATCTGTGCCC 
AAAACTATTG CCAAGACTGA AGGAGCCATG AGAATTTATA CAACTCAGAG AGACTTTGTT 
GGCCTTGCTC CTCTTGTGAT GGACACCGAG ATCCTGAACA CTGCTGTTCT GACTGGAAAG 
AAAGTTATGA TGCCTGTGAG GACGGTAGCT GTGGAGGAAG ATGGAGTGGT CACAGATGTT 
TCTGACTACA CAGACTGCAG CTCCACTGAT GAAGACGTTC TCAAGGTGTC AGACAGATGT 
GACTATGTTT TTGTGAATGG CAAGGAGACC AAAGGCAAGG TGAAGATGAT AGTAAACTTC 
ACCTACAGCT ACCTGAGTGC TCAGCTCGAA CTAAATGTGT GGATCCCTCG GCTCCCTCTG 
CAGATTGAGT TGTCAGACAC AGAACTGAGC CAGATTAAAA GCTGGAGAGT TCCTATTCTG 
ACCTCGAAAA GATCTAGCTG GAACAATGAT GAGGATGAAC GAAAGGGCAA AGGCTGCATG 
CTCCAGCTTC AACACGCACT TGTGCGGGTT CTTACCCATT TTGTGGCTGA ACAGGAAGAC 
CCCCGCGACC CTACAGCCTA TTTCCTGGGC TCTGATTGGC AGGTGGATAT TACAAGGTTG 
GTACGATACT TCATGAAGGT CGAGGACACT CGAGTTGCAA GACTTCAGGA AGGAAGAGTG 
CTGTCAGGCC GAGACTTTGG GACGACAAAC ATCCAGGTTT TTTCTCCACT GTCTGATGTC 
ATCTTGGCAA AAACAACAGT CAAAGTTGTG GATGACAAAG TGAGCATCAC CGAATTAGGT 
GTTCAAGTGG TTACTGGACT ATCCATGACG TTACAACGTA GTTCAGGAAG TAGCAGGGCC 
ATCTTGGCTA CCACAACCAC ACAAGAGGTT CTACAAAGCC CCAAACAGGA AGCTTTGGTC 
AGTCCGTGGG TCCAGTTCAG TGATGGCAAC CAGACACCTC TTGACATTTA TGATCCAGCA 
AACTACCGAA TGACAGTGAC GTCTCTAGAT CAGGGGGTGG TGTCAGTGCA GGGTAGACCT 
CCTGTCGTAG TGGCGGAGGG TGAGGGTGAA GGAGTCTTAG TCAGGGTAGA GATGTCCATC 
TGTGAGTCCT GCCAAAAGTC TAAACGCAAA AGTACTGTGG CTGTGGGCAA TGGCAGCCTT 
AAGGTCAAGT TTCAGGCCAA CACACGGCGG CCAGGTGGTA GTACCCGCAG CGCGGACCGA 
AGCCCTGCTG GCAACAAAGA ACGCAACAAT GACTATGGGA ATGATGGGGA AGAGATGGAC 
AGTGAGAAGA AGCAAAGGAT GCCATCTCAG TTTCTTCCTT CAAGTATTCC AAACCCCAAC 
AAAGAGGAGA GTGTCATTCA GAAGATCCCA ACAACGATCA AATCTACAGA GAGAACCATG 
ATAACCAGCG GCGGTCTCGG AGGAGCGGGC AAGACAAGAA GACCAGAAAA TGTTGGCAGT 
CCGACCAGCG TAATGAATTT CAGCCTAATA AGCAGCCCCA GAGACAGCAG CAAATCATAT 
AGCTCAGACA ACATGATTGG GGAGGATATG AGCAGTGTTA GTTTTACCAG CACTGTGAAA 
GCTCCTGGGA ATCTAGTAAA CTACAACAAC TTCCCCACCA AGGTAGAGAT CCCTGGACCA 
GAAATGCCAG AGGTAGAAAG TGGTGAAGAA GCTTTGGCCA ACAGGCCTCT CACAGACTTG 
GAGATTGGCA TGTATGCCTT GTTGGGTGTT TTTTGCCTTG CCATCTTGGT TTTTCTTGTG 
AACTGTATTT CATATGTTGT AAAATTCAGG CACAAGAAGC CTCCCTCTCA TGGCCAGGAG 
CCAACAGGGC ACAGGCACGA CTGGGTCTGG CTGGGCACAG ACGCTGAGCT GGTGATGACC 
GTGCCATGCA GTCCGGTCCA GCAAGACTCA AATACAGCAA CCACTGTCAT CGACATTGGG 
CCCGACAAGA CTGCCTCTCT TTCCAGGAGG CCCAGTTGCC TGGCCTCTGT ATCGGACTCT 
CCTCTCAGCG GTGCCAGCTC TCACAGGACC AAACCCATAC AAAGCGAGTC CCTCCACTCG 
CCCACCAGCA AGAGAAAGAG AGTTCAGTTC ACCACGTTCT CCACACTGGA GCGCCAGCAT 
TCGCCACACC TGTCACCCAA GGAAAATGGT CATGGTATCC ACTGGGTTGG GAAGGAAGAG 
TGCAATGGAG AGGAACCTCA AGTGCCCATC ATAAAGCCTG GGGACCAGTT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012721683.1
CDS	306..3719
Translation	MSVTWQSWMVPLLVLATVPAVLTQDLNSKEVTDIKSPLSFPVSLPVNYEVRDADYLFLKEAGQDFMRNSSMQSHTQPFVLLRASRQPVVNASYGPVSTERPVPLDLVQAVQLFNAPNVFTFNWKIQAFVLTPRVFSSKPKVRVLFYVAGRDWDRGEGAVDELPCVTVFAFWQTQEVRGSCAIGGERGTCMAELSPEPGWFAAGSEGISRERQDPSAGNPVELYYQAQPRVNGKCFSVNGSRWSSSAQPAEYVPVTPMQRIGSVQLLQIPKGMATLSRLKLGNAIVIRTSSKPLKKTDIATFYILMAGSAQLTNFTLRASVKKGVTFRTATPSNSLLWDITLDMGADGAIAVICQRKTPIPGKRLESSLLEVLQMDFEVEELSSPVDSQVIIWKLELPSVPKTIAKTEGAMRIYTTQRDFVGLAPLVMDTEILNTAVLTGKKVMMPVRTVAVEEDGVVTDVSDYTDCSSTDEDVLKVSDRCDYVFVNGKETKGKVKMIVNFTYSYLSAQLELNVWIPRLPLQIELSDTELSQIKSWRVPILTSKRSSWNNDEDERKGKGCMLQLQHALVRVLTHFVAEQEDPRDPTAYFLGSDWQVDITRLVRYFMKVEDTRVARLQEGRVLSGRDFGTTNIQVFSPLSDVILAKTTVKVVDDKVSITELGVQVVTGLSMTLQRSSGSSRAILATTTTQEVLQSPKQEALVSPWVQFSDGNQTPLDIYDPANYRMTVTSLDQGVVSVQGRPPVVVAEGEGEGVLVRVEMSICESCQKSKRKSTVAVGNGSLKVKFQANTRRPGGSTRSADRSPAGNKERNNDYGNDGEEMDSEKKQRMPSQFLPSSIPNPNKEESVIQKIPTTIKSTERTMITSGGLGGAGKTRRPENVGSPTSVMNFSLISSPRDSSKSYSSDNMIGEDMSSVSFTSTVKAPGNLVNYNNFPTKVEIPGPEMPEVESGEEALANRPLTDLEIGMYALLGVFCLAILVFLVNCISYVVKFRHKKPPSHGQEPTGHRHDWVWLGTDAELVMTVPCSPVQQDSNTATTVIDIGPDKTASLSRRPSCLASVSDSPLSGASSHRTKPIQSESLHSPTSKRKRVQFTTFSTLERQHSPHLSPKENGHGIHWVGKEECNGEEPQVPIIKPGDQL

Target ORF information:

RefSeq Version	XM_012866229.2
Organism	Fundulus heteroclitus(mummichog)
Definition	Fundulus heteroclitus transmembrane protein 132D-like (LOC105928766), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012866229.2

ORF Insert Sequence:

ATGAGCGTTA CCTGGCAAAG CTGGATGGTC CCGTTGTTAG TGCTTGCAAC AGTACCAGCA 
GTACTTACAC AAGATCTAAA CAGCAAGGAA GTCACTGACA TTAAGAGTCC ATTGTCTTTC 
CCTGTCTCCC TGCCGGTCAA CTATGAGGTA CGAGATGCCG ACTACCTCTT CCTCAAGGAG 
GCTGGACAGG ACTTCATGAG GAACTCCAGC ATGCAGAGTC ACACCCAGCC CTTTGTTTTA 
TTAAGAGCCA GCCGCCAGCC GGTTGTCAAT GCCAGCTATG GTCCCGTGTC CACAGAGAGA 
CCCGTGCCTC TCGATCTGGT TCAGGCTGTG CAGCTCTTTA ACGCTCCAAA TGTCTTTACA 
TTTAACTGGA AAATCCAGGC CTTTGTGCTA ACGCCTCGGG TTTTCTCCTC CAAACCCAAA 
GTTCGGGTTC TCTTCTACGT CGCAGGCAGG GATTGGGACA GAGGCGAGGG AGCCGTGGAT 
GAGCTACCTT GTGTGACGGT TTTCGCTTTC TGGCAAACGC AGGAGGTGAG GGGTTCTTGT 
GCAATCGGAG GCGAGAGGGG AACTTGCATG GCTGAGCTGT CGCCTGAGCC TGGCTGGTTC 
GCTGCAGGGT CAGAGGGCAT CAGCAGGGAG AGGCAGGATC CGTCTGCTGG GAACCCAGTG 
GAGCTGTACT ACCAGGCCCA GCCCAGGGTC AATGGGAAGT GTTTTTCTGT GAACGGAAGC 
CGCTGGAGCA GCTCGGCGCA GCCGGCCGAG TATGTTCCTG TGACTCCTAT GCAGAGGATC 
GGAAGCGTGC AACTTCTGCA GATTCCCAAA GGGATGGCGA CGCTCTCTCG CCTCAAGCTA 
GGGAACGCGA TAGTCATTCG GACGTCATCC AAGCCTCTGA AAAAGACCGA CATCGCCACC 
TTCTACATAC TGATGGCCGG CTCCGCTCAG CTGACTAACT TCACTCTCAG AGCATCAGTA 
AAGAAAGGTG TGACCTTTCG AACAGCGACA CCCAGCAACT CTTTACTGTG GGACATCACG 
CTCGATATGG GAGCAGATGG TGCCATTGCT GTTATTTGTC AGAGAAAGAC CCCCATACCT 
GGGAAAAGGC TTGAAAGCAG TCTGCTGGAG GTTCTTCAAA TGGATTTTGA GGTAGAAGAA 
CTGAGCAGTC CCGTTGACAG CCAGGTGATC ATATGGAAGC TGGAGCTACC ATCTGTGCCC 
AAAACTATTG CCAAGACTGA AGGAGCCATG AGAATTTATA CAACTCAGAG AGACTTTGTT 
GGCCTTGCTC CTCTTGTGAT GGACACCGAG ATCCTGAACA CTGCTGTTCT GACTGGAAAG 
AAAGTTATGA TGCCTGTGAG GACGGTAGCT GTGGAGGAAG ATGGAGTGGT CACAGATGTT 
TCTGACTACA CAGACTGCAG CTCCACTGAT GAAGACGTTC TCAAGGTGTC AGACAGATGT 
GACTATGTTT TTGTGAATGG CAAGGAGACC AAAGGCAAGG TGAAGATGAT AGTAAACTTC 
ACCTACAGCT ACCTGAGTGC TCAGCTCGAA CTAAATGTGT GGATCCCTCG GCTCCCTCTG 
CAGATTGAGT TGTCAGACAC AGAACTGAGC CAGATTAAAA GCTGGAGAGT TCCTATTCTG 
ACCTCGAAAA GATCTAGCTG GAACAATGAT GAGGATGAAC GAAAGGGCAA AGGCTGCATG 
CTCCAGCTTC AACACGCACT TGTGCGGGTT CTTACCCATT TTGTGGCTGA ACAGGAAGAC 
CCCCGCGACC CTACAGCCTA TTTCCTGGGC TCTGATTGGC AGGTGGATAT TACAAGGTTG 
GTACGATACT TCATGAAGGT CGAGGACACT CGAGTTGCAA GACTTCAGGA AGGAAGAGTG 
CTGTCAGGCC GAGACTTTGG GACGACAAAC ATCCAGGTTT TTTCTCCACT GTCTGATGTC 
ATCTTGGCAA AAACAACAGT CAAAGTTGTG GATGACAAAG TGAGCATCAC CGAATTAGGT 
GTTCAAGTGG TTACTGGACT ATCCATGACG TTACAACGTA GTTCAGGAAG TAGCAGGGCC 
ATCTTGGCTA CCACAACCAC ACAAGAGGTT CTACAAAGCC CCAAACAGGA AGCTTTGGTC 
AGTCCGTGGG TCCAGTTCAG TGATGGCAAC CAGACACCTC TTGACATTTA TGATCCAGCA 
AACTACCGAA TGACAGTGAC GTCTCTAGAT CAGGGGGTGG TGTCAGTGCA GGGTAGACCT 
CCTGTCGTAG TGGCGGAGGG TGAGGGTGAA GGAGTCTTAG TCAGGGTAGA GATGTCCATC 
TGTGAGTCCT GCCAAAAGTC TAAACGCAAA AGTACTGTGG CTGTGGGCAA TGGCAGCCTT 
AAGGTCAAGT TTCAGGCCAA CACACGGCGG CCAGGTGGTA GTACCCGCAG CGCGGACCGA 
AGCCCTGCTG GCAACAAAGA ACGCAACAAT GACTATGGGA ATGATGGGGA AGAGATGGAC 
AGTGAGAAGA AGCAAAGGAT GCCATCTCAG TTTCTTCCTT CAAGTATTCC AAACCCCAAC 
AAAGAGGAGA GTGTCATTCA GAAGATCCCA ACAACGATCA AATCTACAGA GAGAACCATG 
ATAACCAGCG GCGGTCTCGG AGGAGCGGGC AAGACAAGAA GACCAGAAAA TGTTGGCAGT 
CCGACCAGCG TAATGAATTT CAGCCTAATA AGCAGCCCCA GAGACAGCAG CAAATCATAT 
AGCTCAGACA ACATGATTGG GGAGGATATG AGCAGTGTTA GTTTTACCAG CACTGTGAAA 
GCTCCTGGGA ATCTAGTAAA CTACAACAAC TTCCCCACCA AGGTAGAGAT CCCTGGACCA 
GAAATGCCAG AGGTAGAAAG TGGTGAAGAA GCTTTGGCCA ACAGGCCTCT CACAGACTTG 
GAGATTGGCA TGTATGCCTT GTTGGGTGTT TTTTGCCTTG CCATCTTGGT TTTTCTTGTG 
AACTGTATTT CATATGTTGT AAAATTCAGG CACAAGAAGC CTCCCTCTCA TGGCCAGGAG 
CCAACAGGGC ACAGGCACGA CTGGGTCTGG CTGGGCACAG ACGCTGAGCT GGTGATGACC 
GTGCCATGCA GTCCGGTCCA GCAAGACTCA AATACAGCAA CCACTGTCAT CGACATTGGG 
CCCGACAAGA CTGCCTCTCT TTCCAGGAGG CCCAGTTGCC TGGCCTCTGT ATCGGACTCT 
CCTCTCAGCG GTGCCAGCTC TCACAGGACC AAACCCATAC AAAGCGAGTC CCTCCACTCG 
CCCACCAGCA AGAGAAAGAG AGTTCAGTTC ACCACGTTCT CCACACTGGA GCGCCAGCAT 
TCGCCACACC TGTCACCCAA GGAAAATGGT CATGGTATCC ACTGGGTTGG GAAGGAAGAG 
TGCAATGGAG AGGAACCTCA AGTGCCCATC ATAAAGCCTG GGGACCAGTT ATAA