LOC105921453 cDNA ORF clone, Fundulus heteroclitus(mummichog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105921453
Entrez Gene ID	105921453
Full Name	protein FAM184A
Synonyms	fam184a
Gene Type	protein-coding
Organism	Fundulus heteroclitus(mummichog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012857173.1	XP_012712627.1	protein FAM184A isoform X5
XM_012857169.1	XP_012712623.1	protein FAM184A isoform X1
XM_012857169.2	XP_012712623.1	protein FAM184A isoform X1
XM_012857170.1	XP_012712624.1	protein FAM184A isoform X2
XM_012857171.1	XP_012712625.1	protein FAM184A isoform X3
XM_012857172.1	XP_012712626.1	protein FAM184A isoform X4
XM_012857170.2	XP_012712624.1	protein FAM184A isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC105921453 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105921453 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFu105780	XM_012857171.1 Latest version!	Fundulus heteroclitus family with sequence similarity 184, member A (fam184a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OFu105782	XM_012857173.1 Latest version!	Fundulus heteroclitus family with sequence similarity 184, member A (fam184a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OFu105778	XM_012857169.2 Latest version!	Fundulus heteroclitus protein FAM184A (LOC105921453), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFu105779	XM_012857170.2 Latest version!	Fundulus heteroclitus protein FAM184A (LOC105921453), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFu105781	XM_012857172.1 Latest version!	Fundulus heteroclitus family with sequence similarity 184, member A (fam184a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
View Old Accession Versions >>
OFu105778	XM_012857169.1	Fundulus heteroclitus family with sequence similarity 184, member A (fam184a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFu105779	XM_012857170.1	Fundulus heteroclitus family with sequence similarity 184, member A (fam184a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105921453 ( XM_012857171.1 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_012712625.1 Fundulus heteroclitus family with sequence similarity 184, member A (fam184a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OFu105780
Clone ID Related Accession (Same CDS sequence)	XM_012857171.1
Accession Version	XM_012857171.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-14
Organism	Fundulus heteroclitus(Mummichog)
Product	protein FAM184A isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012225189.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACGG GGGCAGGCTG GCAGCCTCCG TACAGCACCT CGACGAGCAC CAGCAAATAC 
GCCCCCTCTC CAACGGCGTC CAACATGTTT TACGACGGGA GCTTGACGCT GGAATACACT 
CCGGACCTGC ACCTGAAGAT GAGCAAGAAG ATCGCGCAGC TGACAAAGGT AATTTATGCT 
CTCAACACCA AGAACGATGA GCACGAGGAG GAAATCGAGT CTCTGAAAGA AGCCCACGAG 
GATGAGGTCC AACACATAGT GACGGAAACC AGAGACAAGA TCATGCAGTA CAGGAGCAAA 
ATGGCGGACG AGGCGGACCT GAGGCGGCGG CTGGCCAGTC TGGAGGAATC TGTCGAACTC 
CACGACCACA TGAAGAGACA GGCCTTGGCA GAGTTCGAGA TGTACAGACA GAGGATGGAG 
GACTCGCAGC TCTGCACAGA GGCCCAGCAT ACCCAGAGGG TGGTGTCCAT GAGCAGAGAG 
GTGGAAGAGA TGCGTCGCGA TTTTGAGGAG AAGCTTCGGG TGTTCAGCCA GGCTCAGGCC 
CAGTTTGAGG CAGATAAGCG GCGGGCGCTG GACGAGCTGA GGGCCACCCA CCGACACGAG 
GTGGAGGAGC TCCGCAACAA CCAGCAGAAC CACAGCGCCA CCTCTGCCGA GGACCAGGAG 
AAACTGGCCG AGCTGCATAG ACAAGAGGTG GAGGCTTTGA TGGAGAGGCT GGAGGAGCTG 
ACGAAGGATA AGGTCCGTCT GGTGGAGGAG TACGAGGCCA AGCTGGCCAA GGCGCAGGAG 
TACTATGAGA GGGAGCTGGA GGCCATGAGG AGGACCCACC AGCTGACCAC TGAGAACCTG 
CTGGCCTGGA AGAGGACGGA GGTGGAGCTG CGGAAGGAGT TCCAGGCTCA GGAGGCGGCG 
CTGCAGCGTT CGCTGTCCAA GCTAAGGAGC GAGCTCCAGA AAGCTCAGGA GGAGGCCCGG 
GAGAACCGCG ACAAAACCAA CAGGCTGCAG ACGTCGCTGG CCAACGCAGA GGGAACCATC 
AAGAACCTGC ACAAGCAGCT GGAGGAAGCC ATCCAGGACG GAGAGATCTG GGTGATGCAG 
CTGAAGGACA CCGAGTATGA ACTGGAGAGC AGCAGGGAGC GCGTTCAGCA GCAGGCTACA 
GAAATCCTCC ACAAAGCCAG TCAGATCGGC TCCTTGCAGG CCACCCAGAT GGCCCACGAG 
GCGACCATCA GGAACCTGGA CCAGGAGCAG AGTCGTCTCA AGGAGAAGAT CGGCCGGCTG 
GAGGAGGAGC GGGAGGCCCT GCTCAACCAG AGCCAGGCCG CCGGCGAGCA GCACAAACAG 
CAGGTGGTGA AACTGGAGCA GTCTCTACGG GAGGAGCACC AGGGCTACGA GAAGGAGCTG 
TCCAGGCTGA GAGCTCACTA CGAGGAGGAG ACGCTGCGCT TTAAGGAGGC CCAGGTCAGA 
GCGCTGGAGG AGCTGGAGGA GAAACACAGG GCCATGAGGG AGGAGGCCCA GCAGGAGAAG 
GAGGATGAGA AGAAGCTCCT CGTCACGAAA ATGAGTCAGG AGTTTGAGAT CAAGCGTCTG 
TCTTTAGAGG AGCAGAGAGA TCGTCTTCAG CAGCAGCTGG ACAACTTAAA GGAGGAGCTT 
GCGGCCAAAG TCAACATGGC CAATCAGGAG GTCTCCCACC TGCAGGAGCT GGTGAGGGAG 
GGGGAGCAGA ACCTGAGCTC AGCTCAGACG CAGATAAGCT GTCTGAAGGA AACCCAGGAG 
AAGCTGCGCA TAGAGCTGGA CGCGACCAGA GGCCGAGTGA GGGAGACCAG CAACCTGCTC 
ACTGACCTAC AGGAGGAGAT CGAGACCCAG AAGCAGCAGC ATGATGCCAG AGTGATGGCC 
ATCCGGACGG AGGAGAAGCA GAAGATGGAC AAGATGGCAG ATGACTTGGA TCAGAAGTGG 
AGAGACGCAC TGCGGGAGGA AGTGCGTTTG CTGAAGGAGG AGCTGAGCGA GGAGTGTGAA 
GCCGACAAGC AGGCAGCTCT CACTCAGCTT TCCCAGCAGA AGGAGCTGGA GCTGATGGCC 
TCAAGAGAAG GCTGGCAGAG GAAAGTGGAA GACCTGCTGG AACAGATATC GCTCCTGAAG 
CAGTCCCTGG AGCTGCAGTT GTCTCAGTCT CAGTCTGCTC TCCAGCAGCT GCAGTCTCAG 
TTCAACCAGG AGAGGGAGCT GCTGAGCCAG CAGCTTAAAG AGATGCAGAG GGAACACCAG 
AGGAGAGAGC ATCGATTACA GGAGGCTCAC TGCTGCGCCC TCAGCACCAT GGAGGAGGCC 
AGACAGCACC AGATCAGAGC CCTGGAGGAG CGTCTGAAGC AGGAGCACAG GGAGGAGGTC 
CATGCTCTGA AGGAGGCCCA CAGGAGGACC TTGGACATCC TCAGGCAGCA GTCAGACCAG 
GAGCTGCAGA CGCTCCGATT TGAGCTGGAG GACGAGGGGA AAGCAAAAAT GGCGTCTCTC 
CGGGCAGAGC TGAACCACCT CCACGCTACG GCCATCGAGC ATTTGAAGCA GATCCACCTC 
AAAGAAAACA GCGCAGCTAA AAGGGAACTG GAGAAGGCGA TGGAGCACAG CCACCAGCAG 
GAACAAGAAC TCCTGGTTCG CATCTCCGAC CTTCAGGCGG ACTTGTGTTC CCGCGGCAAC 
CGCATCACAG ACCTGGACCA CGAAATCCAC TCTCTGAACG AGACCATCGA CACGCTGACC 
AGAGAGCTGG AGCTGAAGGG CAAAGAGGTG CTGCGAGTCC GCAGCGAAGC CAACCATCAG 
ATAAGGGCTC ATGAGCAAGA CCTCATAAAG AGGCACGAGA GGGACCTGGA CGAGATGAAA 
GTAGTTCACC ACAGAGAAGT GCAGATCATG CTGGCCGACT TCAACAAGGC CCAGGAGGTG 
CTCAAAGACA AGATTTCCGC TCTGCAGATC CTGTTGGAGG GTACGGAGGA GAAGTTGAGG 
AACAGAGAAA GCCGACCAGA AGATCTCCAC CTCATAGCAG AGCTCAGAGA GATGGTGACT 
GAAAGAGAAG CTCTGGTCAA AAAGCTGGTC GACGACAAGA AGTTCTACCA GCTGGAGCTG 
GTGAACAGAG AGACGGGCTT CAACAAGGTC TTCAATTCCA GTGCCAACGT TGGTGTCATC 
AACCCTCTTA TCAAGTCAAA AACAGGCAGC CAATCAGATC AGCGCCTCAC CAGCTTTCCT 
AGCCATTCTG CTCTCCGATT CGTCAGCCCT CCCACCATGA GCCACGAAGG CCAGGAAGAG 
GGGGGGCGGG AGGCGGAGGA GCCTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012712625.1
CDS	192..3461
Translation	MATGAGWQPPYSTSTSTSKYAPSPTASNMFYDGSLTLEYTPDLHLKMSKKIAQLTKVIYALNTKNDEHEEEIESLKEAHEDEVQHIVTETRDKIMQYRSKMADEADLRRRLASLEESVELHDHMKRQALAEFEMYRQRMEDSQLCTEAQHTQRVVSMSREVEEMRRDFEEKLRVFSQAQAQFEADKRRALDELRATHRHEVEELRNNQQNHSATSAEDQEKLAELHRQEVEALMERLEELTKDKVRLVEEYEAKLAKAQEYYERELEAMRRTHQLTTENLLAWKRTEVELRKEFQAQEAALQRSLSKLRSELQKAQEEARENRDKTNRLQTSLANAEGTIKNLHKQLEEAIQDGEIWVMQLKDTEYELESSRERVQQQATEILHKASQIGSLQATQMAHEATIRNLDQEQSRLKEKIGRLEEEREALLNQSQAAGEQHKQQVVKLEQSLREEHQGYEKELSRLRAHYEEETLRFKEAQVRALEELEEKHRAMREEAQQEKEDEKKLLVTKMSQEFEIKRLSLEEQRDRLQQQLDNLKEELAAKVNMANQEVSHLQELVREGEQNLSSAQTQISCLKETQEKLRIELDATRGRVRETSNLLTDLQEEIETQKQQHDARVMAIRTEEKQKMDKMADDLDQKWRDALREEVRLLKEELSEECEADKQAALTQLSQQKELELMASREGWQRKVEDLLEQISLLKQSLELQLSQSQSALQQLQSQFNQERELLSQQLKEMQREHQRREHRLQEAHCCALSTMEEARQHQIRALEERLKQEHREEVHALKEAHRRTLDILRQQSDQELQTLRFELEDEGKAKMASLRAELNHLHATAIEHLKQIHLKENSAAKRELEKAMEHSHQQEQELLVRISDLQADLCSRGNRITDLDHEIHSLNETIDTLTRELELKGKEVLRVRSEANHQIRAHEQDLIKRHERDLDEMKVVHHREVQIMLADFNKAQEVLKDKISALQILLEGTEEKLRNRESRPEDLHLIAELREMVTEREALVKKLVDDKKFYQLELVNRETGFNKVFNSSANVGVINPLIKSKTGSQSDQRLTSFPSHSALRFVSPPTMSHEGQEEGGREAEEPS

Target ORF information:

RefSeq Version	XM_012857171.1
Organism	Fundulus heteroclitus(Mummichog)
Definition	Fundulus heteroclitus family with sequence similarity 184, member A (fam184a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012857171.1

ORF Insert Sequence:

ATGGCGACGG GGGCAGGCTG GCAGCCTCCG TACAGCACCT CGACGAGCAC CAGCAAATAC 
GCCCCCTCTC CAACGGCGTC CAACATGTTT TACGACGGGA GCTTGACGCT GGAATACACT 
CCGGACCTGC ACCTGAAGAT GAGCAAGAAG ATCGCGCAGC TGACAAAGGT AATTTATGCT 
CTCAACACCA AGAACGATGA GCACGAGGAG GAAATCGAGT CTCTGAAAGA AGCCCACGAG 
GATGAGGTCC AACACATAGT GACGGAAACC AGAGACAAGA TCATGCAGTA CAGGAGCAAA 
ATGGCGGACG AGGCGGACCT GAGGCGGCGG CTGGCCAGTC TGGAGGAATC TGTCGAACTC 
CACGACCACA TGAAGAGACA GGCCTTGGCA GAGTTCGAGA TGTACAGACA GAGGATGGAG 
GACTCGCAGC TCTGCACAGA GGCCCAGCAT ACCCAGAGGG TGGTGTCCAT GAGCAGAGAG 
GTGGAAGAGA TGCGTCGCGA TTTTGAGGAG AAGCTTCGGG TGTTCAGCCA GGCTCAGGCC 
CAGTTTGAGG CAGATAAGCG GCGGGCGCTG GACGAGCTGA GGGCCACCCA CCGACACGAG 
GTGGAGGAGC TCCGCAACAA CCAGCAGAAC CACAGCGCCA CCTCTGCCGA GGACCAGGAG 
AAACTGGCCG AGCTGCATAG ACAAGAGGTG GAGGCTTTGA TGGAGAGGCT GGAGGAGCTG 
ACGAAGGATA AGGTCCGTCT GGTGGAGGAG TACGAGGCCA AGCTGGCCAA GGCGCAGGAG 
TACTATGAGA GGGAGCTGGA GGCCATGAGG AGGACCCACC AGCTGACCAC TGAGAACCTG 
CTGGCCTGGA AGAGGACGGA GGTGGAGCTG CGGAAGGAGT TCCAGGCTCA GGAGGCGGCG 
CTGCAGCGTT CGCTGTCCAA GCTAAGGAGC GAGCTCCAGA AAGCTCAGGA GGAGGCCCGG 
GAGAACCGCG ACAAAACCAA CAGGCTGCAG ACGTCGCTGG CCAACGCAGA GGGAACCATC 
AAGAACCTGC ACAAGCAGCT GGAGGAAGCC ATCCAGGACG GAGAGATCTG GGTGATGCAG 
CTGAAGGACA CCGAGTATGA ACTGGAGAGC AGCAGGGAGC GCGTTCAGCA GCAGGCTACA 
GAAATCCTCC ACAAAGCCAG TCAGATCGGC TCCTTGCAGG CCACCCAGAT GGCCCACGAG 
GCGACCATCA GGAACCTGGA CCAGGAGCAG AGTCGTCTCA AGGAGAAGAT CGGCCGGCTG 
GAGGAGGAGC GGGAGGCCCT GCTCAACCAG AGCCAGGCCG CCGGCGAGCA GCACAAACAG 
CAGGTGGTGA AACTGGAGCA GTCTCTACGG GAGGAGCACC AGGGCTACGA GAAGGAGCTG 
TCCAGGCTGA GAGCTCACTA CGAGGAGGAG ACGCTGCGCT TTAAGGAGGC CCAGGTCAGA 
GCGCTGGAGG AGCTGGAGGA GAAACACAGG GCCATGAGGG AGGAGGCCCA GCAGGAGAAG 
GAGGATGAGA AGAAGCTCCT CGTCACGAAA ATGAGTCAGG AGTTTGAGAT CAAGCGTCTG 
TCTTTAGAGG AGCAGAGAGA TCGTCTTCAG CAGCAGCTGG ACAACTTAAA GGAGGAGCTT 
GCGGCCAAAG TCAACATGGC CAATCAGGAG GTCTCCCACC TGCAGGAGCT GGTGAGGGAG 
GGGGAGCAGA ACCTGAGCTC AGCTCAGACG CAGATAAGCT GTCTGAAGGA AACCCAGGAG 
AAGCTGCGCA TAGAGCTGGA CGCGACCAGA GGCCGAGTGA GGGAGACCAG CAACCTGCTC 
ACTGACCTAC AGGAGGAGAT CGAGACCCAG AAGCAGCAGC ATGATGCCAG AGTGATGGCC 
ATCCGGACGG AGGAGAAGCA GAAGATGGAC AAGATGGCAG ATGACTTGGA TCAGAAGTGG 
AGAGACGCAC TGCGGGAGGA AGTGCGTTTG CTGAAGGAGG AGCTGAGCGA GGAGTGTGAA 
GCCGACAAGC AGGCAGCTCT CACTCAGCTT TCCCAGCAGA AGGAGCTGGA GCTGATGGCC 
TCAAGAGAAG GCTGGCAGAG GAAAGTGGAA GACCTGCTGG AACAGATATC GCTCCTGAAG 
CAGTCCCTGG AGCTGCAGTT GTCTCAGTCT CAGTCTGCTC TCCAGCAGCT GCAGTCTCAG 
TTCAACCAGG AGAGGGAGCT GCTGAGCCAG CAGCTTAAAG AGATGCAGAG GGAACACCAG 
AGGAGAGAGC ATCGATTACA GGAGGCTCAC TGCTGCGCCC TCAGCACCAT GGAGGAGGCC 
AGACAGCACC AGATCAGAGC CCTGGAGGAG CGTCTGAAGC AGGAGCACAG GGAGGAGGTC 
CATGCTCTGA AGGAGGCCCA CAGGAGGACC TTGGACATCC TCAGGCAGCA GTCAGACCAG 
GAGCTGCAGA CGCTCCGATT TGAGCTGGAG GACGAGGGGA AAGCAAAAAT GGCGTCTCTC 
CGGGCAGAGC TGAACCACCT CCACGCTACG GCCATCGAGC ATTTGAAGCA GATCCACCTC 
AAAGAAAACA GCGCAGCTAA AAGGGAACTG GAGAAGGCGA TGGAGCACAG CCACCAGCAG 
GAACAAGAAC TCCTGGTTCG CATCTCCGAC CTTCAGGCGG ACTTGTGTTC CCGCGGCAAC 
CGCATCACAG ACCTGGACCA CGAAATCCAC TCTCTGAACG AGACCATCGA CACGCTGACC 
AGAGAGCTGG AGCTGAAGGG CAAAGAGGTG CTGCGAGTCC GCAGCGAAGC CAACCATCAG 
ATAAGGGCTC ATGAGCAAGA CCTCATAAAG AGGCACGAGA GGGACCTGGA CGAGATGAAA 
GTAGTTCACC ACAGAGAAGT GCAGATCATG CTGGCCGACT TCAACAAGGC CCAGGAGGTG 
CTCAAAGACA AGATTTCCGC TCTGCAGATC CTGTTGGAGG GTACGGAGGA GAAGTTGAGG 
AACAGAGAAA GCCGACCAGA AGATCTCCAC CTCATAGCAG AGCTCAGAGA GATGGTGACT 
GAAAGAGAAG CTCTGGTCAA AAAGCTGGTC GACGACAAGA AGTTCTACCA GCTGGAGCTG 
GTGAACAGAG AGACGGGCTT CAACAAGGTC TTCAATTCCA GTGCCAACGT TGGTGTCATC 
AACCCTCTTA TCAAGTCAAA AACAGGCAGC CAATCAGATC AGCGCCTCAC CAGCTTTCCT 
AGCCATTCTG CTCTCCGATT CGTCAGCCCT CCCACCATGA GCCACGAAGG CCAGGAAGAG 
GGGGGGCGGG AGGCGGAGGA GCCTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105921453 ( XM_012857173.1 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_012712627.1 Fundulus heteroclitus family with sequence similarity 184, member A (fam184a), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OFu105782
Clone ID Related Accession (Same CDS sequence)	XM_012857173.1
Accession Version	XM_012857173.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3144bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-14
Organism	Fundulus heteroclitus(Mummichog)
Product	protein FAM184A isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012225189.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACGG GGGCAGGCTG GCAGCCTCCG TACAGCACCT CGACGAGCAC CAGCAAATAC 
GCCCCCTCTC CAACGGCGTC CAACATGTTT TACGACGGGA GCTTGACGCT GGAATACACT 
CCGGACCTGC ACCTGAAGAT GAGCAAGAAG ATCGCGCAGC TGACAAAGGT AATTTATGCT 
CTCAACACCA AGAACGATGA GCACGAGGAG GAAATCGAGT CTCTGAAAGA AGCCCACGAG 
GATGAGGTCC AACACATAGT GACGGAAACC AGAGACAAGA TCATGCAGTA CAGGAGCAAA 
ATGGCGGACG AGGCGGACCT GAGGCGGCGG CTGGCCAGTC TGGAGGAATC TGTCGAACTC 
CACGACCACA TGAAGAGACA GGCCTTGGCA GAGTTCGAGA TGTACAGACA GAGGATGGAG 
GACTCGCAGC TCTGCACAGA GGCCCAGCAT ACCCAGAGGG TGGTGTCCAT GAGCAGAGAG 
GTGGAAGAGA TGCGTCGCGA TTTTGAGGAG AAGCTTCGGG TGTTCAGCCA GGCTCAGGCC 
CAGTTTGAGG CAGATAAGCG GCGGGCGCTG GACGAGCTGA GGGCCACCCA CCGACACGAG 
GTGGAGGAGC TCCGCAACAA CCAGCAGAAC CACAGCGCCA CCTCTGCCGA GGACCAGGAG 
AAACTGGCCG AGCTGCATAG ACAAGAGGTG GAGGCTTTGA TGGAGAGGCT GGAGGAGCTG 
ACGAAGGATA AGGTCCGTCT GGTGGAGGAG TACGAGGCCA AGCTGGCCAA GGCGCAGGAG 
TACTATGAGA GGGAGCTGGA GGCCATGAGG AGGACCCACC AGCTGACCAC TGAGAACCTG 
CTGGCCTGGA AGAGGACGGA GGTGGAGCTG CGGAAGGAGT TCCAGGCTCA GGAGGCGGCG 
CTGCAGCGTT CGCTGTCCAA GCTAAGGAGC GAGCTCCAGA AAGCTCAGGA GGAGGCCCGG 
GAGAACCGCG ACAAAACCAA CAGGCTGCAG ACGTCGCTGG CCAACGCAGA GGGAACCATC 
AAGAACCTGC ACAAGCAGCT GGAGGAAGCC ATCCAGGACG GAGAGATCTG GGTGATGCAG 
CTGAAGGACA CCGAGTATGA ACTGGAGAGC AGCAGGGAGC GCGTTCAGCA GCAGGCTACA 
GAAATCCTCC ACAAAGCCAG TCAGATCGGC TCCTTGCAGG CCACCCAGAT GGCCCACGAG 
GCGACCATCA GGAACCTGGA CCAGGAGCAG AGTCGTCTCA AGGAGAAGAT CGGCCGGCTG 
GAGGAGGAGC GGGAGGCCCT GCTCAACCAG AGCCAGGCCG CCGGCGAGCA GCACAAACAG 
CAGGTGGTGA AACTGGAGCA GTCTCTACGG GAGGAGCACC AGGGCTACGA GAAGGAGCTG 
TCCAGGCTGA GAGCTCACTA CGAGGAGGAG ACGCTGCGCT TTAAGGAGGC CCAGGTCAGA 
GCGCTGGAGG AGCTGGAGGA GAAACACAGG GCCATGAGGG AGGAGGCCCA GCAGGAGAAG 
GAGGATGAGA AGAAGCTCCT CGTCACGAAA ATGAGTCAGG AGTTTGAGAT CAAGCGTCTG 
TCTTTAGAGG AGCAGAGAGA TCGTCTTCAG CAGCAGCTGG ACAACTTAAA GGAGGAGCTT 
GCGGCCAAAG TCAACATGGC CAATCAGGAG GTCTCCCACC TGCAGGAGCT GGTGAGGGAG 
GGGGAGCAGA ACCTGAGCTC AGCTCAGACG CAGATAAGCT GTCTGAAGGA AACCCAGGAG 
AAGCTGCGCA TAGAGCTGGA CGCGACCAGA GGCCGAGTGA GGGAGACCAG CAACCTGCTC 
ACTGACCTAC AGGAGGAGAT CGAGACCCAG AAGCAGCAGC ATGATGCCAG AGTGATGGCC 
ATCCGGACGG AGGAGAAGCA GAAGATGGAC AAGATGGCAG ATGACTTGGA TCAGAAGTGG 
AGAGACGCAC TGCGGGAGGA AGTGCGTTTG CTGAAGGAGG AGCTGAGCGA GGAGTGTGAA 
GCCGACAAGC AGGCAGCTCT CACTCAGCTT TCCCAGCAGA AGGAGCTGGA GCTGATGGCC 
TCAAGAGAAG GCTGGCAGAG GAAAGTGGAA GACCTGCTGG AACAGATATC GCTCCTGAAG 
CAGTCCCTGG AGCTGCAGTT GTCTCAGTCT CAGTCTGCTC TCCAGCAGCT GCAGTCTCAG 
TTCAACCAGG AGAGGGAGCT GCTGAGCCAG CAGCTTAAAG AGATGCAGAG GGAACACCAG 
AGGAGAGAGC ATCGATTACA GGAGGCTCAC TGCTGCGCCC TCAGCACCAT GGAGGAGGCC 
AGACAGCACC AGATCAGAGC CCTGGAGGAG CGTCTGAAGC AGGAGCACAG GGAGGAGGTC 
CATGCTCTGA AGGAGGCCCA CAGGAGGACC TTGGACATCC TCAGGCAGCA GTCAGACCAG 
GAGCTGCAGA CGCTCCGATT TGAGCTGGAG GACGAGGGGA AAGCAAAAAT GGCGTCTCTC 
CGGGCAGAGC TGAACCACCT CCACGCTACG GCCATCGAGC ATTTGAAGCA GATCCACCTC 
AAAGAAAACA GCGCAGCTAA AAGGGAACTG GAGAAGGCGA TGGAGCACAG CCACCAGCAG 
GAACAAGAAC TCCTGGTTCG CATCTCCGAC CTTCAGGCGG ACTTGTGTTC CCGCGGCAAC 
CGCATCACAG ACCTGGACCA CGAAATCCAC TCTCTGAACG AGACCATCGA CACGCTGACC 
AGAGAGCTGG AGCTGAAGGG CAAAGAGGTG CTGCGAGTCC GCAGCGAAGC CAACCATCAG 
ATAAGGGCTC ATGAGCAAGA CCTCATAAAG AGGCACGAGA GGGACCTGGA CGAGATGAAA 
GTAGTTCACC ACAGAGAAGT GCAGATCATG CTGGCCGACT TCAACAAGGC CCAGGAGGTG 
CTCAAAGACA AGATTTCCGC TCTGCAGATC CTGTTGGAGG GTACGGAGGA GAAGTTGAGG 
AACAGAGAAA GCCGACCAGA AGATCTCCAC CTCATAGCAG AGCTCAGAGA GATGGTGACT 
GAAAGAGAAG CTCTGGTCAA AAAGCTGGTC GACGACAAGA AGTTCTACCA GCTGGAGCTG 
GTGAACAGAG AGACGGGCTT CAACAAGGTC TTCAATTCCA GTGCCAACGT TGGTGTCATC 
AACCCTCTTA TCAAGCCTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012712627.1
CDS	192..3335
Translation	MATGAGWQPPYSTSTSTSKYAPSPTASNMFYDGSLTLEYTPDLHLKMSKKIAQLTKVIYALNTKNDEHEEEIESLKEAHEDEVQHIVTETRDKIMQYRSKMADEADLRRRLASLEESVELHDHMKRQALAEFEMYRQRMEDSQLCTEAQHTQRVVSMSREVEEMRRDFEEKLRVFSQAQAQFEADKRRALDELRATHRHEVEELRNNQQNHSATSAEDQEKLAELHRQEVEALMERLEELTKDKVRLVEEYEAKLAKAQEYYERELEAMRRTHQLTTENLLAWKRTEVELRKEFQAQEAALQRSLSKLRSELQKAQEEARENRDKTNRLQTSLANAEGTIKNLHKQLEEAIQDGEIWVMQLKDTEYELESSRERVQQQATEILHKASQIGSLQATQMAHEATIRNLDQEQSRLKEKIGRLEEEREALLNQSQAAGEQHKQQVVKLEQSLREEHQGYEKELSRLRAHYEEETLRFKEAQVRALEELEEKHRAMREEAQQEKEDEKKLLVTKMSQEFEIKRLSLEEQRDRLQQQLDNLKEELAAKVNMANQEVSHLQELVREGEQNLSSAQTQISCLKETQEKLRIELDATRGRVRETSNLLTDLQEEIETQKQQHDARVMAIRTEEKQKMDKMADDLDQKWRDALREEVRLLKEELSEECEADKQAALTQLSQQKELELMASREGWQRKVEDLLEQISLLKQSLELQLSQSQSALQQLQSQFNQERELLSQQLKEMQREHQRREHRLQEAHCCALSTMEEARQHQIRALEERLKQEHREEVHALKEAHRRTLDILRQQSDQELQTLRFELEDEGKAKMASLRAELNHLHATAIEHLKQIHLKENSAAKRELEKAMEHSHQQEQELLVRISDLQADLCSRGNRITDLDHEIHSLNETIDTLTRELELKGKEVLRVRSEANHQIRAHEQDLIKRHERDLDEMKVVHHREVQIMLADFNKAQEVLKDKISALQILLEGTEEKLRNRESRPEDLHLIAELREMVTEREALVKKLVDDKKFYQLELVNRETGFNKVFNSSANVGVINPLIKPS

Target ORF information:

RefSeq Version	XM_012857173.1
Organism	Fundulus heteroclitus(Mummichog)
Definition	Fundulus heteroclitus family with sequence similarity 184, member A (fam184a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012857173.1

ORF Insert Sequence:

ATGGCGACGG GGGCAGGCTG GCAGCCTCCG TACAGCACCT CGACGAGCAC CAGCAAATAC 
GCCCCCTCTC CAACGGCGTC CAACATGTTT TACGACGGGA GCTTGACGCT GGAATACACT 
CCGGACCTGC ACCTGAAGAT GAGCAAGAAG ATCGCGCAGC TGACAAAGGT AATTTATGCT 
CTCAACACCA AGAACGATGA GCACGAGGAG GAAATCGAGT CTCTGAAAGA AGCCCACGAG 
GATGAGGTCC AACACATAGT GACGGAAACC AGAGACAAGA TCATGCAGTA CAGGAGCAAA 
ATGGCGGACG AGGCGGACCT GAGGCGGCGG CTGGCCAGTC TGGAGGAATC TGTCGAACTC 
CACGACCACA TGAAGAGACA GGCCTTGGCA GAGTTCGAGA TGTACAGACA GAGGATGGAG 
GACTCGCAGC TCTGCACAGA GGCCCAGCAT ACCCAGAGGG TGGTGTCCAT GAGCAGAGAG 
GTGGAAGAGA TGCGTCGCGA TTTTGAGGAG AAGCTTCGGG TGTTCAGCCA GGCTCAGGCC 
CAGTTTGAGG CAGATAAGCG GCGGGCGCTG GACGAGCTGA GGGCCACCCA CCGACACGAG 
GTGGAGGAGC TCCGCAACAA CCAGCAGAAC CACAGCGCCA CCTCTGCCGA GGACCAGGAG 
AAACTGGCCG AGCTGCATAG ACAAGAGGTG GAGGCTTTGA TGGAGAGGCT GGAGGAGCTG 
ACGAAGGATA AGGTCCGTCT GGTGGAGGAG TACGAGGCCA AGCTGGCCAA GGCGCAGGAG 
TACTATGAGA GGGAGCTGGA GGCCATGAGG AGGACCCACC AGCTGACCAC TGAGAACCTG 
CTGGCCTGGA AGAGGACGGA GGTGGAGCTG CGGAAGGAGT TCCAGGCTCA GGAGGCGGCG 
CTGCAGCGTT CGCTGTCCAA GCTAAGGAGC GAGCTCCAGA AAGCTCAGGA GGAGGCCCGG 
GAGAACCGCG ACAAAACCAA CAGGCTGCAG ACGTCGCTGG CCAACGCAGA GGGAACCATC 
AAGAACCTGC ACAAGCAGCT GGAGGAAGCC ATCCAGGACG GAGAGATCTG GGTGATGCAG 
CTGAAGGACA CCGAGTATGA ACTGGAGAGC AGCAGGGAGC GCGTTCAGCA GCAGGCTACA 
GAAATCCTCC ACAAAGCCAG TCAGATCGGC TCCTTGCAGG CCACCCAGAT GGCCCACGAG 
GCGACCATCA GGAACCTGGA CCAGGAGCAG AGTCGTCTCA AGGAGAAGAT CGGCCGGCTG 
GAGGAGGAGC GGGAGGCCCT GCTCAACCAG AGCCAGGCCG CCGGCGAGCA GCACAAACAG 
CAGGTGGTGA AACTGGAGCA GTCTCTACGG GAGGAGCACC AGGGCTACGA GAAGGAGCTG 
TCCAGGCTGA GAGCTCACTA CGAGGAGGAG ACGCTGCGCT TTAAGGAGGC CCAGGTCAGA 
GCGCTGGAGG AGCTGGAGGA GAAACACAGG GCCATGAGGG AGGAGGCCCA GCAGGAGAAG 
GAGGATGAGA AGAAGCTCCT CGTCACGAAA ATGAGTCAGG AGTTTGAGAT CAAGCGTCTG 
TCTTTAGAGG AGCAGAGAGA TCGTCTTCAG CAGCAGCTGG ACAACTTAAA GGAGGAGCTT 
GCGGCCAAAG TCAACATGGC CAATCAGGAG GTCTCCCACC TGCAGGAGCT GGTGAGGGAG 
GGGGAGCAGA ACCTGAGCTC AGCTCAGACG CAGATAAGCT GTCTGAAGGA AACCCAGGAG 
AAGCTGCGCA TAGAGCTGGA CGCGACCAGA GGCCGAGTGA GGGAGACCAG CAACCTGCTC 
ACTGACCTAC AGGAGGAGAT CGAGACCCAG AAGCAGCAGC ATGATGCCAG AGTGATGGCC 
ATCCGGACGG AGGAGAAGCA GAAGATGGAC AAGATGGCAG ATGACTTGGA TCAGAAGTGG 
AGAGACGCAC TGCGGGAGGA AGTGCGTTTG CTGAAGGAGG AGCTGAGCGA GGAGTGTGAA 
GCCGACAAGC AGGCAGCTCT CACTCAGCTT TCCCAGCAGA AGGAGCTGGA GCTGATGGCC 
TCAAGAGAAG GCTGGCAGAG GAAAGTGGAA GACCTGCTGG AACAGATATC GCTCCTGAAG 
CAGTCCCTGG AGCTGCAGTT GTCTCAGTCT CAGTCTGCTC TCCAGCAGCT GCAGTCTCAG 
TTCAACCAGG AGAGGGAGCT GCTGAGCCAG CAGCTTAAAG AGATGCAGAG GGAACACCAG 
AGGAGAGAGC ATCGATTACA GGAGGCTCAC TGCTGCGCCC TCAGCACCAT GGAGGAGGCC 
AGACAGCACC AGATCAGAGC CCTGGAGGAG CGTCTGAAGC AGGAGCACAG GGAGGAGGTC 
CATGCTCTGA AGGAGGCCCA CAGGAGGACC TTGGACATCC TCAGGCAGCA GTCAGACCAG 
GAGCTGCAGA CGCTCCGATT TGAGCTGGAG GACGAGGGGA AAGCAAAAAT GGCGTCTCTC 
CGGGCAGAGC TGAACCACCT CCACGCTACG GCCATCGAGC ATTTGAAGCA GATCCACCTC 
AAAGAAAACA GCGCAGCTAA AAGGGAACTG GAGAAGGCGA TGGAGCACAG CCACCAGCAG 
GAACAAGAAC TCCTGGTTCG CATCTCCGAC CTTCAGGCGG ACTTGTGTTC CCGCGGCAAC 
CGCATCACAG ACCTGGACCA CGAAATCCAC TCTCTGAACG AGACCATCGA CACGCTGACC 
AGAGAGCTGG AGCTGAAGGG CAAAGAGGTG CTGCGAGTCC GCAGCGAAGC CAACCATCAG 
ATAAGGGCTC ATGAGCAAGA CCTCATAAAG AGGCACGAGA GGGACCTGGA CGAGATGAAA 
GTAGTTCACC ACAGAGAAGT GCAGATCATG CTGGCCGACT TCAACAAGGC CCAGGAGGTG 
CTCAAAGACA AGATTTCCGC TCTGCAGATC CTGTTGGAGG GTACGGAGGA GAAGTTGAGG 
AACAGAGAAA GCCGACCAGA AGATCTCCAC CTCATAGCAG AGCTCAGAGA GATGGTGACT 
GAAAGAGAAG CTCTGGTCAA AAAGCTGGTC GACGACAAGA AGTTCTACCA GCTGGAGCTG 
GTGAACAGAG AGACGGGCTT CAACAAGGTC TTCAATTCCA GTGCCAACGT TGGTGTCATC 
AACCCTCTTA TCAAGCCTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105921453 ( XM_012857169.1 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_012712623.1 Fundulus heteroclitus family with sequence similarity 184, member A (fam184a), transcript variant X1, mRNA.

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CloneID	OFu105778
Clone ID Related Accession (Same CDS sequence)	XM_012857169.1 , XM_012857169.2
Accession Version	XM_012857169.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-14
Organism	Fundulus heteroclitus(Mummichog)
Product	protein FAM184A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012225189.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACGG GGGCAGGCTG GCAGCCTCCG TACAGCACCT CGACGAGCAC CAGCAAATAC 
GCCCCCTCTC CAACGGCGTC CAACATGTTT TACGACGGGA GCTTGACGCT GGAATACACT 
CCGGACCTGC ACCTGAAGAT GAGCAAGAAG ATCGCGCAGC TGACAAAGGT AATTTATGCT 
CTCAACACCA AGAACGATGA GCACGAGGAG GAAATCGAGT CTCTGAAAGA AGCCCACGAG 
GATGAGGTCC AACACATAGT GACGGAAACC AGAGACAAGA TCATGCAGTA CAGGAGCAAA 
ATGGCGGACG AGGCGGACCT GAGGCGGCGG CTGGCCAGTC TGGAGGAATC TGTCGAACTC 
CACGACCACA TGAAGAGACA GGCCTTGGCA GAGTTCGAGA TGTACAGACA GAGGATGGAG 
GACTCGCAGC TCTGCACAGA GGCCCAGCAT ACCCAGAGGG TGGTGTCCAT GAGCAGAGAG 
GTGGAAGAGA TGCGTCGCGA TTTTGAGGAG AAGCTTCGGG TGTTCAGCCA GGCTCAGGCC 
CAGTTTGAGG CAGATAAGCG GCGGGCGCTG GACGAGCTGA GGGCCACCCA CCGACACGAG 
GTGGAGGAGC TCCGCAACAA CCAGCAGAAC CACAGCGCCA CCTCTGCCGA GGACCAGGAG 
AAACTGGCCG AGCTGCATAG ACAAGAGGTG GAGGCTTTGA TGGAGAGGCT GGAGGAGCTG 
ACGAAGGATA AGGTCCGTCT GGTGGAGGAG TACGAGGCCA AGCTGGCCAA GGCGCAGGAG 
TACTATGAGA GGGAGCTGGA GGCCATGAGG AGGACCCACC AGCTGACCAC TGAGAACCTG 
CTGGCCTGGA AGAGGACGGA GGTGGAGCTG CGGAAGGAGT TCCAGGCTCA GGAGGCGGCG 
CTGCAGCGTT CGCTGTCCAA GCTAAGGAGC GAGCTCCAGA AAGCTCAGGA GGAGGCCCGG 
GAGAACCGCG ACAAAACCAA CAGGCTGCAG ACGTCGCTGG CCAACGCAGA GGGAACCATC 
AAGAACCTGC ACAAGCAGCT GGAGGAAGCC ATCCAGGACG GAGAGATCTG GGTGATGCAG 
CTGAAGGACA CCGAGTATGA ACTGGAGAGC AGCAGGGAGC GCGTTCAGCA GCAGGCTACA 
GAAATCCTCC ACAAAGCCAG TCAGATCGGC TCCTTGCAGG CCACCCAGAT GGCCCACGAG 
GCGACCATCA GGAACCTGGA CCAGGAGCAG AGTCGTCTCA AGGAGAAGAT CGGCCGGCTG 
GAGGAGGAGC GGGAGGCCCT GCTCAACCAG AGCCAGGCCG CCGGCGAGCA GCACAAACAG 
CAGGTGGTGA AACTGGAGCA GTCTCTACGG GAGGAGCACC AGGGCTACGA GAAGGAGCTG 
TCCAGGCTGA GAGCTCACTA CGAGGAGGAG ACGCTGCGCT TTAAGGAGGC CCAGGTCAGA 
GCGCTGGAGG AGCTGGAGGA GAAACACAGG GCCATGAGGG AGGAGGCCCA GCAGGAGAAG 
GAGGATGAGA AGAAGCTCCT CGTCACGAAA ATGAGTCAGG AGTTTGAGAT CAAGCGTCTG 
TCTTTAGAGG AGCAGAGAGA TCGTCTTCAG CAGCAGCTGG ACAACTTAAA GGAGGAGCTT 
GCGGCCAAAG TCAACATGGC CAATCAGGAG GTCTCCCACC TGCAGGAGCT GGTGAGGGAG 
GGGGAGCAGA ACCTGAGCTC AGCTCAGACG CAGATAAGCT GTCTGAAGGA AACCCAGGAG 
AAGCTGCGCA TAGAGCTGGA CGCGACCAGA GGCCGAGTGA GGGAGACCAG CAACCTGCTC 
ACTGACCTAC AGGAGGAGAT CGAGACCCAG AAGCAGCAGC ATGATGCCAG AGTGATGGCC 
ATCCGGACGG AGGAGAAGCA GAAGATGGAC AAGATGGCAG ATGACTTGGA TCAGAAGTGG 
AGAGACGCAC TGCGGGAGGA AGTGCGTTTG CTGAAGGAGG AGCTGAGCGA GGAGTGTGAA 
GCCGACAAGC AGGCAGCTCT CACTCAGCTT TCCCAGCAGA AGGAGCTGGA GCTGATGGCC 
TCAAGAGAAG GCTGGCAGAG GAAAGTGGAA GACCTGCTGG AACAGATATC GCTCCTGAAG 
CAGTCCCTGG AGCTGCAGTT GTCTCAGTCT CAGTCTGCTC TCCAGCAGCT GCAGTCTCAG 
TTCAACCAGG AGAGGGAGCT GCTGAGCCAG CAGCTTAAAG AGATGCAGAG GGAACACCAG 
AGGAGAGAGC ATCGATTACA GGAGGCTCAC TGCTGCGCCC TCAGCACCAT GGAGGAGGCC 
AGACAGCACC AGATCAGAGC CCTGGAGGAG CGTCTGAAGC AGGAGCACAG GGAGGAGGTC 
CATGCTCTGA AGGAGGCCCA CAGGAGGACC TTGGACATCC TCAGGCAGCA GTCAGACCAG 
GAGCTGCAGA CGCTCCGATT TGAGCTGGAG GACGAGGGGA AAGCAAAAAT GGCGTCTCTC 
CGGGCAGAGC TGAACCACCT CCACGCTACG GCCATCGAGC ATTTGAAGCA GATCCACCTC 
AAAGAAAACA GCGCAGCTAA AAGGGAACTG GAGAAGGCGA TGGAGCACAG CCACCAGCAG 
GAACAAGAAC TCCTGGTTCG CATCTCCGAC CTTCAGGCGG ACTTGTGTTC CCGCGGCAAC 
CGCATCACAG ACCTGGACCA CGAAATCCAC TCTCTGAACG AGACCATCGA CACGCTGACC 
AGAGAGCTGG AGCTGAAGGG CAAAGAGGTG CTGCGAGTCC GCAGCGAAGC CAACCATCAG 
ATAAGGGCTC ATGAGCAAGA CCTCATAAAG AGGCACGAGA GGGACCTGGA CGAGATGAAA 
GTAGTTCACC ACAGAGAAGT GCAGATCATG CTGGCCGACT TCAACAAGGC CCAGGAGGTG 
CTCAAAGACA AGATTTCCGC TCTGCAGATC CTGTTGGAGG GTACGGAGGA GAAGTTGAGG 
AACAGAGAAA GCCGACCAGA AGATCTCCAC CTCATAGCAG AGCTCAGAGA GATGGTGACT 
GAAAGAGAAG CTCTGGTCAA AAAGCTGGTC GACGACAAGA AGTTCTACCA GCTGGAGCTG 
GTGAACAGAG AGACGGGCTT CAACAAGGTC TTCAATTCCA GTGCCAACGT TGGTGTCATC 
AACCCTCTTA TCAAGTCAAA AACAGGCAGC CAATCAGATC AGCGCCTCAC CAGCTTTCCT 
AGCCATTCTG CTCTCCGATT CGTCAGCCCT CCCACCATGA GCCACGAAGG CCAGGAAGAG 
GGGGGGCGGG AGGCGGAGGA GGTAGGACCG GGAGGCCGCT ATGCGCTTCC TCACTCCCCC 
CTCCAGAGGT CCCTCCCAAG GTTTAAGAAA GCCCTGCCAC TGTTCAGCCC TCTTCCTCCT 
CCTCCTTCTT CTTCTACTCC TCTACCTCAG CATCCTCCTC CTCCTCATCC CCCACACCAT 
CCCATGCCGA GCCTCGCTCC TCCTCATGCT TCTCAGGATC CTCTCGTGCA GCATCAGGGC 
CTGCAGCGCT GTGGGAGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012712623.1
CDS	192..3692
Translation	MATGAGWQPPYSTSTSTSKYAPSPTASNMFYDGSLTLEYTPDLHLKMSKKIAQLTKVIYALNTKNDEHEEEIESLKEAHEDEVQHIVTETRDKIMQYRSKMADEADLRRRLASLEESVELHDHMKRQALAEFEMYRQRMEDSQLCTEAQHTQRVVSMSREVEEMRRDFEEKLRVFSQAQAQFEADKRRALDELRATHRHEVEELRNNQQNHSATSAEDQEKLAELHRQEVEALMERLEELTKDKVRLVEEYEAKLAKAQEYYERELEAMRRTHQLTTENLLAWKRTEVELRKEFQAQEAALQRSLSKLRSELQKAQEEARENRDKTNRLQTSLANAEGTIKNLHKQLEEAIQDGEIWVMQLKDTEYELESSRERVQQQATEILHKASQIGSLQATQMAHEATIRNLDQEQSRLKEKIGRLEEEREALLNQSQAAGEQHKQQVVKLEQSLREEHQGYEKELSRLRAHYEEETLRFKEAQVRALEELEEKHRAMREEAQQEKEDEKKLLVTKMSQEFEIKRLSLEEQRDRLQQQLDNLKEELAAKVNMANQEVSHLQELVREGEQNLSSAQTQISCLKETQEKLRIELDATRGRVRETSNLLTDLQEEIETQKQQHDARVMAIRTEEKQKMDKMADDLDQKWRDALREEVRLLKEELSEECEADKQAALTQLSQQKELELMASREGWQRKVEDLLEQISLLKQSLELQLSQSQSALQQLQSQFNQERELLSQQLKEMQREHQRREHRLQEAHCCALSTMEEARQHQIRALEERLKQEHREEVHALKEAHRRTLDILRQQSDQELQTLRFELEDEGKAKMASLRAELNHLHATAIEHLKQIHLKENSAAKRELEKAMEHSHQQEQELLVRISDLQADLCSRGNRITDLDHEIHSLNETIDTLTRELELKGKEVLRVRSEANHQIRAHEQDLIKRHERDLDEMKVVHHREVQIMLADFNKAQEVLKDKISALQILLEGTEEKLRNRESRPEDLHLIAELREMVTEREALVKKLVDDKKFYQLELVNRETGFNKVFNSSANVGVINPLIKSKTGSQSDQRLTSFPSHSALRFVSPPTMSHEGQEEGGREAEEVGPGGRYALPHSPLQRSLPRFKKALPLFSPLPPPPSSSTPLPQHPPPPHPPHHPMPSLAPPHASQDPLVQHQGLQRCGR

Target ORF information:

RefSeq Version	XM_012857169.1
Organism	Fundulus heteroclitus(Mummichog)
Definition	Fundulus heteroclitus family with sequence similarity 184, member A (fam184a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012857169.1

ORF Insert Sequence:

ATGGCGACGG GGGCAGGCTG GCAGCCTCCG TACAGCACCT CGACGAGCAC CAGCAAATAC 
GCCCCCTCTC CAACGGCGTC CAACATGTTT TACGACGGGA GCTTGACGCT GGAATACACT 
CCGGACCTGC ACCTGAAGAT GAGCAAGAAG ATCGCGCAGC TGACAAAGGT AATTTATGCT 
CTCAACACCA AGAACGATGA GCACGAGGAG GAAATCGAGT CTCTGAAAGA AGCCCACGAG 
GATGAGGTCC AACACATAGT GACGGAAACC AGAGACAAGA TCATGCAGTA CAGGAGCAAA 
ATGGCGGACG AGGCGGACCT GAGGCGGCGG CTGGCCAGTC TGGAGGAATC TGTCGAACTC 
CACGACCACA TGAAGAGACA GGCCTTGGCA GAGTTCGAGA TGTACAGACA GAGGATGGAG 
GACTCGCAGC TCTGCACAGA GGCCCAGCAT ACCCAGAGGG TGGTGTCCAT GAGCAGAGAG 
GTGGAAGAGA TGCGTCGCGA TTTTGAGGAG AAGCTTCGGG TGTTCAGCCA GGCTCAGGCC 
CAGTTTGAGG CAGATAAGCG GCGGGCGCTG GACGAGCTGA GGGCCACCCA CCGACACGAG 
GTGGAGGAGC TCCGCAACAA CCAGCAGAAC CACAGCGCCA CCTCTGCCGA GGACCAGGAG 
AAACTGGCCG AGCTGCATAG ACAAGAGGTG GAGGCTTTGA TGGAGAGGCT GGAGGAGCTG 
ACGAAGGATA AGGTCCGTCT GGTGGAGGAG TACGAGGCCA AGCTGGCCAA GGCGCAGGAG 
TACTATGAGA GGGAGCTGGA GGCCATGAGG AGGACCCACC AGCTGACCAC TGAGAACCTG 
CTGGCCTGGA AGAGGACGGA GGTGGAGCTG CGGAAGGAGT TCCAGGCTCA GGAGGCGGCG 
CTGCAGCGTT CGCTGTCCAA GCTAAGGAGC GAGCTCCAGA AAGCTCAGGA GGAGGCCCGG 
GAGAACCGCG ACAAAACCAA CAGGCTGCAG ACGTCGCTGG CCAACGCAGA GGGAACCATC 
AAGAACCTGC ACAAGCAGCT GGAGGAAGCC ATCCAGGACG GAGAGATCTG GGTGATGCAG 
CTGAAGGACA CCGAGTATGA ACTGGAGAGC AGCAGGGAGC GCGTTCAGCA GCAGGCTACA 
GAAATCCTCC ACAAAGCCAG TCAGATCGGC TCCTTGCAGG CCACCCAGAT GGCCCACGAG 
GCGACCATCA GGAACCTGGA CCAGGAGCAG AGTCGTCTCA AGGAGAAGAT CGGCCGGCTG 
GAGGAGGAGC GGGAGGCCCT GCTCAACCAG AGCCAGGCCG CCGGCGAGCA GCACAAACAG 
CAGGTGGTGA AACTGGAGCA GTCTCTACGG GAGGAGCACC AGGGCTACGA GAAGGAGCTG 
TCCAGGCTGA GAGCTCACTA CGAGGAGGAG ACGCTGCGCT TTAAGGAGGC CCAGGTCAGA 
GCGCTGGAGG AGCTGGAGGA GAAACACAGG GCCATGAGGG AGGAGGCCCA GCAGGAGAAG 
GAGGATGAGA AGAAGCTCCT CGTCACGAAA ATGAGTCAGG AGTTTGAGAT CAAGCGTCTG 
TCTTTAGAGG AGCAGAGAGA TCGTCTTCAG CAGCAGCTGG ACAACTTAAA GGAGGAGCTT 
GCGGCCAAAG TCAACATGGC CAATCAGGAG GTCTCCCACC TGCAGGAGCT GGTGAGGGAG 
GGGGAGCAGA ACCTGAGCTC AGCTCAGACG CAGATAAGCT GTCTGAAGGA AACCCAGGAG 
AAGCTGCGCA TAGAGCTGGA CGCGACCAGA GGCCGAGTGA GGGAGACCAG CAACCTGCTC 
ACTGACCTAC AGGAGGAGAT CGAGACCCAG AAGCAGCAGC ATGATGCCAG AGTGATGGCC 
ATCCGGACGG AGGAGAAGCA GAAGATGGAC AAGATGGCAG ATGACTTGGA TCAGAAGTGG 
AGAGACGCAC TGCGGGAGGA AGTGCGTTTG CTGAAGGAGG AGCTGAGCGA GGAGTGTGAA 
GCCGACAAGC AGGCAGCTCT CACTCAGCTT TCCCAGCAGA AGGAGCTGGA GCTGATGGCC 
TCAAGAGAAG GCTGGCAGAG GAAAGTGGAA GACCTGCTGG AACAGATATC GCTCCTGAAG 
CAGTCCCTGG AGCTGCAGTT GTCTCAGTCT CAGTCTGCTC TCCAGCAGCT GCAGTCTCAG 
TTCAACCAGG AGAGGGAGCT GCTGAGCCAG CAGCTTAAAG AGATGCAGAG GGAACACCAG 
AGGAGAGAGC ATCGATTACA GGAGGCTCAC TGCTGCGCCC TCAGCACCAT GGAGGAGGCC 
AGACAGCACC AGATCAGAGC CCTGGAGGAG CGTCTGAAGC AGGAGCACAG GGAGGAGGTC 
CATGCTCTGA AGGAGGCCCA CAGGAGGACC TTGGACATCC TCAGGCAGCA GTCAGACCAG 
GAGCTGCAGA CGCTCCGATT TGAGCTGGAG GACGAGGGGA AAGCAAAAAT GGCGTCTCTC 
CGGGCAGAGC TGAACCACCT CCACGCTACG GCCATCGAGC ATTTGAAGCA GATCCACCTC 
AAAGAAAACA GCGCAGCTAA AAGGGAACTG GAGAAGGCGA TGGAGCACAG CCACCAGCAG 
GAACAAGAAC TCCTGGTTCG CATCTCCGAC CTTCAGGCGG ACTTGTGTTC CCGCGGCAAC 
CGCATCACAG ACCTGGACCA CGAAATCCAC TCTCTGAACG AGACCATCGA CACGCTGACC 
AGAGAGCTGG AGCTGAAGGG CAAAGAGGTG CTGCGAGTCC GCAGCGAAGC CAACCATCAG 
ATAAGGGCTC ATGAGCAAGA CCTCATAAAG AGGCACGAGA GGGACCTGGA CGAGATGAAA 
GTAGTTCACC ACAGAGAAGT GCAGATCATG CTGGCCGACT TCAACAAGGC CCAGGAGGTG 
CTCAAAGACA AGATTTCCGC TCTGCAGATC CTGTTGGAGG GTACGGAGGA GAAGTTGAGG 
AACAGAGAAA GCCGACCAGA AGATCTCCAC CTCATAGCAG AGCTCAGAGA GATGGTGACT 
GAAAGAGAAG CTCTGGTCAA AAAGCTGGTC GACGACAAGA AGTTCTACCA GCTGGAGCTG 
GTGAACAGAG AGACGGGCTT CAACAAGGTC TTCAATTCCA GTGCCAACGT TGGTGTCATC 
AACCCTCTTA TCAAGTCAAA AACAGGCAGC CAATCAGATC AGCGCCTCAC CAGCTTTCCT 
AGCCATTCTG CTCTCCGATT CGTCAGCCCT CCCACCATGA GCCACGAAGG CCAGGAAGAG 
GGGGGGCGGG AGGCGGAGGA GGTAGGACCG GGAGGCCGCT ATGCGCTTCC TCACTCCCCC 
CTCCAGAGGT CCCTCCCAAG GTTTAAGAAA GCCCTGCCAC TGTTCAGCCC TCTTCCTCCT 
CCTCCTTCTT CTTCTACTCC TCTACCTCAG CATCCTCCTC CTCCTCATCC CCCACACCAT 
CCCATGCCGA GCCTCGCTCC TCCTCATGCT TCTCAGGATC CTCTCGTGCA GCATCAGGGC 
CTGCAGCGCT GTGGGAGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105921453 ( XM_012857169.2 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_012712623.1 Fundulus heteroclitus protein FAM184A (LOC105921453), transcript variant X1, mRNA.

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CloneID	OFu105778
Clone ID Related Accession (Same CDS sequence)	XM_012857169.1 , XM_012857169.2
Accession Version	XM_012857169.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-31
Organism	Fundulus heteroclitus(mummichog)
Product	protein FAM184A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012225189.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 1, 2017 this sequence version replaced XM_012857169.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACGG GGGCAGGCTG GCAGCCTCCG TACAGCACCT CGACGAGCAC CAGCAAATAC 
GCCCCCTCTC CAACGGCGTC CAACATGTTT TACGACGGGA GCTTGACGCT GGAATACACT 
CCGGACCTGC ACCTGAAGAT GAGCAAGAAG ATCGCGCAGC TGACAAAGGT AATTTATGCT 
CTCAACACCA AGAACGATGA GCACGAGGAG GAAATCGAGT CTCTGAAAGA AGCCCACGAG 
GATGAGGTCC AACACATAGT GACGGAAACC AGAGACAAGA TCATGCAGTA CAGGAGCAAA 
ATGGCGGACG AGGCGGACCT GAGGCGGCGG CTGGCCAGTC TGGAGGAATC TGTCGAACTC 
CACGACCACA TGAAGAGACA GGCCTTGGCA GAGTTCGAGA TGTACAGACA GAGGATGGAG 
GACTCGCAGC TCTGCACAGA GGCCCAGCAT ACCCAGAGGG TGGTGTCCAT GAGCAGAGAG 
GTGGAAGAGA TGCGTCGCGA TTTTGAGGAG AAGCTTCGGG TGTTCAGCCA GGCTCAGGCC 
CAGTTTGAGG CAGATAAGCG GCGGGCGCTG GACGAGCTGA GGGCCACCCA CCGACACGAG 
GTGGAGGAGC TCCGCAACAA CCAGCAGAAC CACAGCGCCA CCTCTGCCGA GGACCAGGAG 
AAACTGGCCG AGCTGCATAG ACAAGAGGTG GAGGCTTTGA TGGAGAGGCT GGAGGAGCTG 
ACGAAGGATA AGGTCCGTCT GGTGGAGGAG TACGAGGCCA AGCTGGCCAA GGCGCAGGAG 
TACTATGAGA GGGAGCTGGA GGCCATGAGG AGGACCCACC AGCTGACCAC TGAGAACCTG 
CTGGCCTGGA AGAGGACGGA GGTGGAGCTG CGGAAGGAGT TCCAGGCTCA GGAGGCGGCG 
CTGCAGCGTT CGCTGTCCAA GCTAAGGAGC GAGCTCCAGA AAGCTCAGGA GGAGGCCCGG 
GAGAACCGCG ACAAAACCAA CAGGCTGCAG ACGTCGCTGG CCAACGCAGA GGGAACCATC 
AAGAACCTGC ACAAGCAGCT GGAGGAAGCC ATCCAGGACG GAGAGATCTG GGTGATGCAG 
CTGAAGGACA CCGAGTATGA ACTGGAGAGC AGCAGGGAGC GCGTTCAGCA GCAGGCTACA 
GAAATCCTCC ACAAAGCCAG TCAGATCGGC TCCTTGCAGG CCACCCAGAT GGCCCACGAG 
GCGACCATCA GGAACCTGGA CCAGGAGCAG AGTCGTCTCA AGGAGAAGAT CGGCCGGCTG 
GAGGAGGAGC GGGAGGCCCT GCTCAACCAG AGCCAGGCCG CCGGCGAGCA GCACAAACAG 
CAGGTGGTGA AACTGGAGCA GTCTCTACGG GAGGAGCACC AGGGCTACGA GAAGGAGCTG 
TCCAGGCTGA GAGCTCACTA CGAGGAGGAG ACGCTGCGCT TTAAGGAGGC CCAGGTCAGA 
GCGCTGGAGG AGCTGGAGGA GAAACACAGG GCCATGAGGG AGGAGGCCCA GCAGGAGAAG 
GAGGATGAGA AGAAGCTCCT CGTCACGAAA ATGAGTCAGG AGTTTGAGAT CAAGCGTCTG 
TCTTTAGAGG AGCAGAGAGA TCGTCTTCAG CAGCAGCTGG ACAACTTAAA GGAGGAGCTT 
GCGGCCAAAG TCAACATGGC CAATCAGGAG GTCTCCCACC TGCAGGAGCT GGTGAGGGAG 
GGGGAGCAGA ACCTGAGCTC AGCTCAGACG CAGATAAGCT GTCTGAAGGA AACCCAGGAG 
AAGCTGCGCA TAGAGCTGGA CGCGACCAGA GGCCGAGTGA GGGAGACCAG CAACCTGCTC 
ACTGACCTAC AGGAGGAGAT CGAGACCCAG AAGCAGCAGC ATGATGCCAG AGTGATGGCC 
ATCCGGACGG AGGAGAAGCA GAAGATGGAC AAGATGGCAG ATGACTTGGA TCAGAAGTGG 
AGAGACGCAC TGCGGGAGGA AGTGCGTTTG CTGAAGGAGG AGCTGAGCGA GGAGTGTGAA 
GCCGACAAGC AGGCAGCTCT CACTCAGCTT TCCCAGCAGA AGGAGCTGGA GCTGATGGCC 
TCAAGAGAAG GCTGGCAGAG GAAAGTGGAA GACCTGCTGG AACAGATATC GCTCCTGAAG 
CAGTCCCTGG AGCTGCAGTT GTCTCAGTCT CAGTCTGCTC TCCAGCAGCT GCAGTCTCAG 
TTCAACCAGG AGAGGGAGCT GCTGAGCCAG CAGCTTAAAG AGATGCAGAG GGAACACCAG 
AGGAGAGAGC ATCGATTACA GGAGGCTCAC TGCTGCGCCC TCAGCACCAT GGAGGAGGCC 
AGACAGCACC AGATCAGAGC CCTGGAGGAG CGTCTGAAGC AGGAGCACAG GGAGGAGGTC 
CATGCTCTGA AGGAGGCCCA CAGGAGGACC TTGGACATCC TCAGGCAGCA GTCAGACCAG 
GAGCTGCAGA CGCTCCGATT TGAGCTGGAG GACGAGGGGA AAGCAAAAAT GGCGTCTCTC 
CGGGCAGAGC TGAACCACCT CCACGCTACG GCCATCGAGC ATTTGAAGCA GATCCACCTC 
AAAGAAAACA GCGCAGCTAA AAGGGAACTG GAGAAGGCGA TGGAGCACAG CCACCAGCAG 
GAACAAGAAC TCCTGGTTCG CATCTCCGAC CTTCAGGCGG ACTTGTGTTC CCGCGGCAAC 
CGCATCACAG ACCTGGACCA CGAAATCCAC TCTCTGAACG AGACCATCGA CACGCTGACC 
AGAGAGCTGG AGCTGAAGGG CAAAGAGGTG CTGCGAGTCC GCAGCGAAGC CAACCATCAG 
ATAAGGGCTC ATGAGCAAGA CCTCATAAAG AGGCACGAGA GGGACCTGGA CGAGATGAAA 
GTAGTTCACC ACAGAGAAGT GCAGATCATG CTGGCCGACT TCAACAAGGC CCAGGAGGTG 
CTCAAAGACA AGATTTCCGC TCTGCAGATC CTGTTGGAGG GTACGGAGGA GAAGTTGAGG 
AACAGAGAAA GCCGACCAGA AGATCTCCAC CTCATAGCAG AGCTCAGAGA GATGGTGACT 
GAAAGAGAAG CTCTGGTCAA AAAGCTGGTC GACGACAAGA AGTTCTACCA GCTGGAGCTG 
GTGAACAGAG AGACGGGCTT CAACAAGGTC TTCAATTCCA GTGCCAACGT TGGTGTCATC 
AACCCTCTTA TCAAGTCAAA AACAGGCAGC CAATCAGATC AGCGCCTCAC CAGCTTTCCT 
AGCCATTCTG CTCTCCGATT CGTCAGCCCT CCCACCATGA GCCACGAAGG CCAGGAAGAG 
GGGGGGCGGG AGGCGGAGGA GGTAGGACCG GGAGGCCGCT ATGCGCTTCC TCACTCCCCC 
CTCCAGAGGT CCCTCCCAAG GTTTAAGAAA GCCCTGCCAC TGTTCAGCCC TCTTCCTCCT 
CCTCCTTCTT CTTCTACTCC TCTACCTCAG CATCCTCCTC CTCCTCATCC CCCACACCAT 
CCCATGCCGA GCCTCGCTCC TCCTCATGCT TCTCAGGATC CTCTCGTGCA GCATCAGGGC 
CTGCAGCGCT GTGGGAGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012712623.1
CDS	350..3850
Translation	MATGAGWQPPYSTSTSTSKYAPSPTASNMFYDGSLTLEYTPDLHLKMSKKIAQLTKVIYALNTKNDEHEEEIESLKEAHEDEVQHIVTETRDKIMQYRSKMADEADLRRRLASLEESVELHDHMKRQALAEFEMYRQRMEDSQLCTEAQHTQRVVSMSREVEEMRRDFEEKLRVFSQAQAQFEADKRRALDELRATHRHEVEELRNNQQNHSATSAEDQEKLAELHRQEVEALMERLEELTKDKVRLVEEYEAKLAKAQEYYERELEAMRRTHQLTTENLLAWKRTEVELRKEFQAQEAALQRSLSKLRSELQKAQEEARENRDKTNRLQTSLANAEGTIKNLHKQLEEAIQDGEIWVMQLKDTEYELESSRERVQQQATEILHKASQIGSLQATQMAHEATIRNLDQEQSRLKEKIGRLEEEREALLNQSQAAGEQHKQQVVKLEQSLREEHQGYEKELSRLRAHYEEETLRFKEAQVRALEELEEKHRAMREEAQQEKEDEKKLLVTKMSQEFEIKRLSLEEQRDRLQQQLDNLKEELAAKVNMANQEVSHLQELVREGEQNLSSAQTQISCLKETQEKLRIELDATRGRVRETSNLLTDLQEEIETQKQQHDARVMAIRTEEKQKMDKMADDLDQKWRDALREEVRLLKEELSEECEADKQAALTQLSQQKELELMASREGWQRKVEDLLEQISLLKQSLELQLSQSQSALQQLQSQFNQERELLSQQLKEMQREHQRREHRLQEAHCCALSTMEEARQHQIRALEERLKQEHREEVHALKEAHRRTLDILRQQSDQELQTLRFELEDEGKAKMASLRAELNHLHATAIEHLKQIHLKENSAAKRELEKAMEHSHQQEQELLVRISDLQADLCSRGNRITDLDHEIHSLNETIDTLTRELELKGKEVLRVRSEANHQIRAHEQDLIKRHERDLDEMKVVHHREVQIMLADFNKAQEVLKDKISALQILLEGTEEKLRNRESRPEDLHLIAELREMVTEREALVKKLVDDKKFYQLELVNRETGFNKVFNSSANVGVINPLIKSKTGSQSDQRLTSFPSHSALRFVSPPTMSHEGQEEGGREAEEVGPGGRYALPHSPLQRSLPRFKKALPLFSPLPPPPSSSTPLPQHPPPPHPPHHPMPSLAPPHASQDPLVQHQGLQRCGR

Target ORF information:

RefSeq Version	XM_012857169.2
Organism	Fundulus heteroclitus(mummichog)
Definition	Fundulus heteroclitus protein FAM184A (LOC105921453), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012857169.2

ORF Insert Sequence:

ATGGCGACGG GGGCAGGCTG GCAGCCTCCG TACAGCACCT CGACGAGCAC CAGCAAATAC 
GCCCCCTCTC CAACGGCGTC CAACATGTTT TACGACGGGA GCTTGACGCT GGAATACACT 
CCGGACCTGC ACCTGAAGAT GAGCAAGAAG ATCGCGCAGC TGACAAAGGT AATTTATGCT 
CTCAACACCA AGAACGATGA GCACGAGGAG GAAATCGAGT CTCTGAAAGA AGCCCACGAG 
GATGAGGTCC AACACATAGT GACGGAAACC AGAGACAAGA TCATGCAGTA CAGGAGCAAA 
ATGGCGGACG AGGCGGACCT GAGGCGGCGG CTGGCCAGTC TGGAGGAATC TGTCGAACTC 
CACGACCACA TGAAGAGACA GGCCTTGGCA GAGTTCGAGA TGTACAGACA GAGGATGGAG 
GACTCGCAGC TCTGCACAGA GGCCCAGCAT ACCCAGAGGG TGGTGTCCAT GAGCAGAGAG 
GTGGAAGAGA TGCGTCGCGA TTTTGAGGAG AAGCTTCGGG TGTTCAGCCA GGCTCAGGCC 
CAGTTTGAGG CAGATAAGCG GCGGGCGCTG GACGAGCTGA GGGCCACCCA CCGACACGAG 
GTGGAGGAGC TCCGCAACAA CCAGCAGAAC CACAGCGCCA CCTCTGCCGA GGACCAGGAG 
AAACTGGCCG AGCTGCATAG ACAAGAGGTG GAGGCTTTGA TGGAGAGGCT GGAGGAGCTG 
ACGAAGGATA AGGTCCGTCT GGTGGAGGAG TACGAGGCCA AGCTGGCCAA GGCGCAGGAG 
TACTATGAGA GGGAGCTGGA GGCCATGAGG AGGACCCACC AGCTGACCAC TGAGAACCTG 
CTGGCCTGGA AGAGGACGGA GGTGGAGCTG CGGAAGGAGT TCCAGGCTCA GGAGGCGGCG 
CTGCAGCGTT CGCTGTCCAA GCTAAGGAGC GAGCTCCAGA AAGCTCAGGA GGAGGCCCGG 
GAGAACCGCG ACAAAACCAA CAGGCTGCAG ACGTCGCTGG CCAACGCAGA GGGAACCATC 
AAGAACCTGC ACAAGCAGCT GGAGGAAGCC ATCCAGGACG GAGAGATCTG GGTGATGCAG 
CTGAAGGACA CCGAGTATGA ACTGGAGAGC AGCAGGGAGC GCGTTCAGCA GCAGGCTACA 
GAAATCCTCC ACAAAGCCAG TCAGATCGGC TCCTTGCAGG CCACCCAGAT GGCCCACGAG 
GCGACCATCA GGAACCTGGA CCAGGAGCAG AGTCGTCTCA AGGAGAAGAT CGGCCGGCTG 
GAGGAGGAGC GGGAGGCCCT GCTCAACCAG AGCCAGGCCG CCGGCGAGCA GCACAAACAG 
CAGGTGGTGA AACTGGAGCA GTCTCTACGG GAGGAGCACC AGGGCTACGA GAAGGAGCTG 
TCCAGGCTGA GAGCTCACTA CGAGGAGGAG ACGCTGCGCT TTAAGGAGGC CCAGGTCAGA 
GCGCTGGAGG AGCTGGAGGA GAAACACAGG GCCATGAGGG AGGAGGCCCA GCAGGAGAAG 
GAGGATGAGA AGAAGCTCCT CGTCACGAAA ATGAGTCAGG AGTTTGAGAT CAAGCGTCTG 
TCTTTAGAGG AGCAGAGAGA TCGTCTTCAG CAGCAGCTGG ACAACTTAAA GGAGGAGCTT 
GCGGCCAAAG TCAACATGGC CAATCAGGAG GTCTCCCACC TGCAGGAGCT GGTGAGGGAG 
GGGGAGCAGA ACCTGAGCTC AGCTCAGACG CAGATAAGCT GTCTGAAGGA AACCCAGGAG 
AAGCTGCGCA TAGAGCTGGA CGCGACCAGA GGCCGAGTGA GGGAGACCAG CAACCTGCTC 
ACTGACCTAC AGGAGGAGAT CGAGACCCAG AAGCAGCAGC ATGATGCCAG AGTGATGGCC 
ATCCGGACGG AGGAGAAGCA GAAGATGGAC AAGATGGCAG ATGACTTGGA TCAGAAGTGG 
AGAGACGCAC TGCGGGAGGA AGTGCGTTTG CTGAAGGAGG AGCTGAGCGA GGAGTGTGAA 
GCCGACAAGC AGGCAGCTCT CACTCAGCTT TCCCAGCAGA AGGAGCTGGA GCTGATGGCC 
TCAAGAGAAG GCTGGCAGAG GAAAGTGGAA GACCTGCTGG AACAGATATC GCTCCTGAAG 
CAGTCCCTGG AGCTGCAGTT GTCTCAGTCT CAGTCTGCTC TCCAGCAGCT GCAGTCTCAG 
TTCAACCAGG AGAGGGAGCT GCTGAGCCAG CAGCTTAAAG AGATGCAGAG GGAACACCAG 
AGGAGAGAGC ATCGATTACA GGAGGCTCAC TGCTGCGCCC TCAGCACCAT GGAGGAGGCC 
AGACAGCACC AGATCAGAGC CCTGGAGGAG CGTCTGAAGC AGGAGCACAG GGAGGAGGTC 
CATGCTCTGA AGGAGGCCCA CAGGAGGACC TTGGACATCC TCAGGCAGCA GTCAGACCAG 
GAGCTGCAGA CGCTCCGATT TGAGCTGGAG GACGAGGGGA AAGCAAAAAT GGCGTCTCTC 
CGGGCAGAGC TGAACCACCT CCACGCTACG GCCATCGAGC ATTTGAAGCA GATCCACCTC 
AAAGAAAACA GCGCAGCTAA AAGGGAACTG GAGAAGGCGA TGGAGCACAG CCACCAGCAG 
GAACAAGAAC TCCTGGTTCG CATCTCCGAC CTTCAGGCGG ACTTGTGTTC CCGCGGCAAC 
CGCATCACAG ACCTGGACCA CGAAATCCAC TCTCTGAACG AGACCATCGA CACGCTGACC 
AGAGAGCTGG AGCTGAAGGG CAAAGAGGTG CTGCGAGTCC GCAGCGAAGC CAACCATCAG 
ATAAGGGCTC ATGAGCAAGA CCTCATAAAG AGGCACGAGA GGGACCTGGA CGAGATGAAA 
GTAGTTCACC ACAGAGAAGT GCAGATCATG CTGGCCGACT TCAACAAGGC CCAGGAGGTG 
CTCAAAGACA AGATTTCCGC TCTGCAGATC CTGTTGGAGG GTACGGAGGA GAAGTTGAGG 
AACAGAGAAA GCCGACCAGA AGATCTCCAC CTCATAGCAG AGCTCAGAGA GATGGTGACT 
GAAAGAGAAG CTCTGGTCAA AAAGCTGGTC GACGACAAGA AGTTCTACCA GCTGGAGCTG 
GTGAACAGAG AGACGGGCTT CAACAAGGTC TTCAATTCCA GTGCCAACGT TGGTGTCATC 
AACCCTCTTA TCAAGTCAAA AACAGGCAGC CAATCAGATC AGCGCCTCAC CAGCTTTCCT 
AGCCATTCTG CTCTCCGATT CGTCAGCCCT CCCACCATGA GCCACGAAGG CCAGGAAGAG 
GGGGGGCGGG AGGCGGAGGA GGTAGGACCG GGAGGCCGCT ATGCGCTTCC TCACTCCCCC 
CTCCAGAGGT CCCTCCCAAG GTTTAAGAAA GCCCTGCCAC TGTTCAGCCC TCTTCCTCCT 
CCTCCTTCTT CTTCTACTCC TCTACCTCAG CATCCTCCTC CTCCTCATCC CCCACACCAT 
CCCATGCCGA GCCTCGCTCC TCCTCATGCT TCTCAGGATC CTCTCGTGCA GCATCAGGGC 
CTGCAGCGCT GTGGGAGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105921453 ( XM_012857170.1 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_012712624.1 Fundulus heteroclitus family with sequence similarity 184, member A (fam184a), transcript variant X2, mRNA.

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CloneID	OFu105779
Clone ID Related Accession (Same CDS sequence)	XM_012857170.1 , XM_012857170.2
Accession Version	XM_012857170.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-14
Organism	Fundulus heteroclitus(Mummichog)
Product	protein FAM184A isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012225189.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACGG GGGCAGGCTG GCAGCCTCCG TACAGCACCT CGACGAGCAC CAGCAAATAC 
GCCCCCTCTC CAACGGCGTC CAACATGTTT TACGACGGGA GCTTGACGCT GGAATACACT 
CCGGACCTGC ACCTGAAGAT GAGCAAGAAG ATCGCGCAGC TGACAAAGGT AATTTATGCT 
CTCAACACCA AGAACGATGA GCACGAGGAG GAAATCGAGT CTCTGAAAGA AGCCCACGAG 
GATGAGGTCC AACACATAGT GACGGAAACC AGAGACAAGA TCATGCAGTA CAGGAGCAAA 
ATGGCGGACG AGGCGGACCT GAGGCGGCGG CTGGCCAGTC TGGAGGAATC TGTCGAACTC 
CACGACCACA TGAAGAGACA GGCCTTGGCA GAGTTCGAGA TGTACAGACA GAGGATGGAG 
GACTCGCAGC TCTGCACAGA GGCCCAGCAT ACCCAGAGGG TGGTGTCCAT GAGCAGAGAG 
GTGGAGGCTT TGATGGAGAG GCTGGAGGAG CTGACGAAGG ATAAGGTCCG TCTGGTGGAG 
GAGTACGAGG CCAAGCTGGC CAAGGCGCAG GAGTACTATG AGAGGGAGCT GGAGGCCATG 
AGGAGGACCC ACCAGCTGAC CACTGAGAAC CTGCTGGCCT GGAAGAGGAC GGAGGTGGAG 
CTGCGGAAGG AGTTCCAGGC TCAGGAGGCG GCGCTGCAGC GTTCGCTGTC CAAGCTAAGG 
AGCGAGCTCC AGAAAGCTCA GGAGGAGGCC CGGGAGAACC GCGACAAAAC CAACAGGCTG 
CAGACGTCGC TGGCCAACGC AGAGGGAACC ATCAAGAACC TGCACAAGCA GCTGGAGGAA 
GCCATCCAGG ACGGAGAGAT CTGGGTGATG CAGCTGAAGG ACACCGAGTA TGAACTGGAG 
AGCAGCAGGG AGCGCGTTCA GCAGCAGGCT ACAGAAATCC TCCACAAAGC CAGTCAGATC 
GGCTCCTTGC AGGCCACCCA GATGGCCCAC GAGGCGACCA TCAGGAACCT GGACCAGGAG 
CAGAGTCGTC TCAAGGAGAA GATCGGCCGG CTGGAGGAGG AGCGGGAGGC CCTGCTCAAC 
CAGAGCCAGG CCGCCGGCGA GCAGCACAAA CAGCAGGTGG TGAAACTGGA GCAGTCTCTA 
CGGGAGGAGC ACCAGGGCTA CGAGAAGGAG CTGTCCAGGC TGAGAGCTCA CTACGAGGAG 
GAGACGCTGC GCTTTAAGGA GGCCCAGGTC AGAGCGCTGG AGGAGCTGGA GGAGAAACAC 
AGGGCCATGA GGGAGGAGGC CCAGCAGGAG AAGGAGGATG AGAAGAAGCT CCTCGTCACG 
AAAATGAGTC AGGAGTTTGA GATCAAGCGT CTGTCTTTAG AGGAGCAGAG AGATCGTCTT 
CAGCAGCAGC TGGACAACTT AAAGGAGGAG CTTGCGGCCA AAGTCAACAT GGCCAATCAG 
GAGGTCTCCC ACCTGCAGGA GCTGGTGAGG GAGGGGGAGC AGAACCTGAG CTCAGCTCAG 
ACGCAGATAA GCTGTCTGAA GGAAACCCAG GAGAAGCTGC GCATAGAGCT GGACGCGACC 
AGAGGCCGAG TGAGGGAGAC CAGCAACCTG CTCACTGACC TACAGGAGGA GATCGAGACC 
CAGAAGCAGC AGCATGATGC CAGAGTGATG GCCATCCGGA CGGAGGAGAA GCAGAAGATG 
GACAAGATGG CAGATGACTT GGATCAGAAG TGGAGAGACG CACTGCGGGA GGAAGTGCGT 
TTGCTGAAGG AGGAGCTGAG CGAGGAGTGT GAAGCCGACA AGCAGGCAGC TCTCACTCAG 
CTTTCCCAGC AGAAGGAGCT GGAGCTGATG GCCTCAAGAG AAGGCTGGCA GAGGAAAGTG 
GAAGACCTGC TGGAACAGAT ATCGCTCCTG AAGCAGTCCC TGGAGCTGCA GTTGTCTCAG 
TCTCAGTCTG CTCTCCAGCA GCTGCAGTCT CAGTTCAACC AGGAGAGGGA GCTGCTGAGC 
CAGCAGCTTA AAGAGATGCA GAGGGAACAC CAGAGGAGAG AGCATCGATT ACAGGAGGCT 
CACTGCTGCG CCCTCAGCAC CATGGAGGAG GCCAGACAGC ACCAGATCAG AGCCCTGGAG 
GAGCGTCTGA AGCAGGAGCA CAGGGAGGAG GTCCATGCTC TGAAGGAGGC CCACAGGAGG 
ACCTTGGACA TCCTCAGGCA GCAGTCAGAC CAGGAGCTGC AGACGCTCCG ATTTGAGCTG 
GAGGACGAGG GGAAAGCAAA AATGGCGTCT CTCCGGGCAG AGCTGAACCA CCTCCACGCT 
ACGGCCATCG AGCATTTGAA GCAGATCCAC CTCAAAGAAA ACAGCGCAGC TAAAAGGGAA 
CTGGAGAAGG CGATGGAGCA CAGCCACCAG CAGGAACAAG AACTCCTGGT TCGCATCTCC 
GACCTTCAGG CGGACTTGTG TTCCCGCGGC AACCGCATCA CAGACCTGGA CCACGAAATC 
CACTCTCTGA ACGAGACCAT CGACACGCTG ACCAGAGAGC TGGAGCTGAA GGGCAAAGAG 
GTGCTGCGAG TCCGCAGCGA AGCCAACCAT CAGATAAGGG CTCATGAGCA AGACCTCATA 
AAGAGGCACG AGAGGGACCT GGACGAGATG AAAGTAGTTC ACCACAGAGA AGTGCAGATC 
ATGCTGGCCG ACTTCAACAA GGCCCAGGAG GTGCTCAAAG ACAAGATTTC CGCTCTGCAG 
ATCCTGTTGG AGGGTACGGA GGAGAAGTTG AGGAACAGAG AAAGCCGACC AGAAGATCTC 
CACCTCATAG CAGAGCTCAG AGAGATGGTG ACTGAAAGAG AAGCTCTGGT CAAAAAGCTG 
GTCGACGACA AGAAGTTCTA CCAGCTGGAG CTGGTGAACA GAGAGACGGG CTTCAACAAG 
GTCTTCAATT CCAGTGCCAA CGTTGGTGTC ATCAACCCTC TTATCAAGTC AAAAACAGGC 
AGCCAATCAG ATCAGCGCCT CACCAGCTTT CCTAGCCATT CTGCTCTCCG ATTCGTCAGC 
CCTCCCACCA TGAGCCACGA AGGCCAGGAA GAGGGGGGGC GGGAGGCGGA GGAGGTAGGA 
CCGGGAGGCC GCTATGCGCT TCCTCACTCC CCCCTCCAGA GGTCCCTCCC AAGGTTTAAG 
AAAGCCCTGC CACTGTTCAG CCCTCTTCCT CCTCCTCCTT CTTCTTCTAC TCCTCTACCT 
CAGCATCCTC CTCCTCCTCA TCCCCCACAC CATCCCATGC CGAGCCTCGC TCCTCCTCAT 
GCTTCTCAGG ATCCTCTCGT GCAGCATCAG GGCCTGCAGC GCTGTGGGAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012712624.1
CDS	192..3485
Translation	MATGAGWQPPYSTSTSTSKYAPSPTASNMFYDGSLTLEYTPDLHLKMSKKIAQLTKVIYALNTKNDEHEEEIESLKEAHEDEVQHIVTETRDKIMQYRSKMADEADLRRRLASLEESVELHDHMKRQALAEFEMYRQRMEDSQLCTEAQHTQRVVSMSREVEALMERLEELTKDKVRLVEEYEAKLAKAQEYYERELEAMRRTHQLTTENLLAWKRTEVELRKEFQAQEAALQRSLSKLRSELQKAQEEARENRDKTNRLQTSLANAEGTIKNLHKQLEEAIQDGEIWVMQLKDTEYELESSRERVQQQATEILHKASQIGSLQATQMAHEATIRNLDQEQSRLKEKIGRLEEEREALLNQSQAAGEQHKQQVVKLEQSLREEHQGYEKELSRLRAHYEEETLRFKEAQVRALEELEEKHRAMREEAQQEKEDEKKLLVTKMSQEFEIKRLSLEEQRDRLQQQLDNLKEELAAKVNMANQEVSHLQELVREGEQNLSSAQTQISCLKETQEKLRIELDATRGRVRETSNLLTDLQEEIETQKQQHDARVMAIRTEEKQKMDKMADDLDQKWRDALREEVRLLKEELSEECEADKQAALTQLSQQKELELMASREGWQRKVEDLLEQISLLKQSLELQLSQSQSALQQLQSQFNQERELLSQQLKEMQREHQRREHRLQEAHCCALSTMEEARQHQIRALEERLKQEHREEVHALKEAHRRTLDILRQQSDQELQTLRFELEDEGKAKMASLRAELNHLHATAIEHLKQIHLKENSAAKRELEKAMEHSHQQEQELLVRISDLQADLCSRGNRITDLDHEIHSLNETIDTLTRELELKGKEVLRVRSEANHQIRAHEQDLIKRHERDLDEMKVVHHREVQIMLADFNKAQEVLKDKISALQILLEGTEEKLRNRESRPEDLHLIAELREMVTEREALVKKLVDDKKFYQLELVNRETGFNKVFNSSANVGVINPLIKSKTGSQSDQRLTSFPSHSALRFVSPPTMSHEGQEEGGREAEEVGPGGRYALPHSPLQRSLPRFKKALPLFSPLPPPPSSSTPLPQHPPPPHPPHHPMPSLAPPHASQDPLVQHQGLQRCGR

Target ORF information:

RefSeq Version	XM_012857170.1
Organism	Fundulus heteroclitus(Mummichog)
Definition	Fundulus heteroclitus family with sequence similarity 184, member A (fam184a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012857170.1

ORF Insert Sequence:

ATGGCGACGG GGGCAGGCTG GCAGCCTCCG TACAGCACCT CGACGAGCAC CAGCAAATAC 
GCCCCCTCTC CAACGGCGTC CAACATGTTT TACGACGGGA GCTTGACGCT GGAATACACT 
CCGGACCTGC ACCTGAAGAT GAGCAAGAAG ATCGCGCAGC TGACAAAGGT AATTTATGCT 
CTCAACACCA AGAACGATGA GCACGAGGAG GAAATCGAGT CTCTGAAAGA AGCCCACGAG 
GATGAGGTCC AACACATAGT GACGGAAACC AGAGACAAGA TCATGCAGTA CAGGAGCAAA 
ATGGCGGACG AGGCGGACCT GAGGCGGCGG CTGGCCAGTC TGGAGGAATC TGTCGAACTC 
CACGACCACA TGAAGAGACA GGCCTTGGCA GAGTTCGAGA TGTACAGACA GAGGATGGAG 
GACTCGCAGC TCTGCACAGA GGCCCAGCAT ACCCAGAGGG TGGTGTCCAT GAGCAGAGAG 
GTGGAGGCTT TGATGGAGAG GCTGGAGGAG CTGACGAAGG ATAAGGTCCG TCTGGTGGAG 
GAGTACGAGG CCAAGCTGGC CAAGGCGCAG GAGTACTATG AGAGGGAGCT GGAGGCCATG 
AGGAGGACCC ACCAGCTGAC CACTGAGAAC CTGCTGGCCT GGAAGAGGAC GGAGGTGGAG 
CTGCGGAAGG AGTTCCAGGC TCAGGAGGCG GCGCTGCAGC GTTCGCTGTC CAAGCTAAGG 
AGCGAGCTCC AGAAAGCTCA GGAGGAGGCC CGGGAGAACC GCGACAAAAC CAACAGGCTG 
CAGACGTCGC TGGCCAACGC AGAGGGAACC ATCAAGAACC TGCACAAGCA GCTGGAGGAA 
GCCATCCAGG ACGGAGAGAT CTGGGTGATG CAGCTGAAGG ACACCGAGTA TGAACTGGAG 
AGCAGCAGGG AGCGCGTTCA GCAGCAGGCT ACAGAAATCC TCCACAAAGC CAGTCAGATC 
GGCTCCTTGC AGGCCACCCA GATGGCCCAC GAGGCGACCA TCAGGAACCT GGACCAGGAG 
CAGAGTCGTC TCAAGGAGAA GATCGGCCGG CTGGAGGAGG AGCGGGAGGC CCTGCTCAAC 
CAGAGCCAGG CCGCCGGCGA GCAGCACAAA CAGCAGGTGG TGAAACTGGA GCAGTCTCTA 
CGGGAGGAGC ACCAGGGCTA CGAGAAGGAG CTGTCCAGGC TGAGAGCTCA CTACGAGGAG 
GAGACGCTGC GCTTTAAGGA GGCCCAGGTC AGAGCGCTGG AGGAGCTGGA GGAGAAACAC 
AGGGCCATGA GGGAGGAGGC CCAGCAGGAG AAGGAGGATG AGAAGAAGCT CCTCGTCACG 
AAAATGAGTC AGGAGTTTGA GATCAAGCGT CTGTCTTTAG AGGAGCAGAG AGATCGTCTT 
CAGCAGCAGC TGGACAACTT AAAGGAGGAG CTTGCGGCCA AAGTCAACAT GGCCAATCAG 
GAGGTCTCCC ACCTGCAGGA GCTGGTGAGG GAGGGGGAGC AGAACCTGAG CTCAGCTCAG 
ACGCAGATAA GCTGTCTGAA GGAAACCCAG GAGAAGCTGC GCATAGAGCT GGACGCGACC 
AGAGGCCGAG TGAGGGAGAC CAGCAACCTG CTCACTGACC TACAGGAGGA GATCGAGACC 
CAGAAGCAGC AGCATGATGC CAGAGTGATG GCCATCCGGA CGGAGGAGAA GCAGAAGATG 
GACAAGATGG CAGATGACTT GGATCAGAAG TGGAGAGACG CACTGCGGGA GGAAGTGCGT 
TTGCTGAAGG AGGAGCTGAG CGAGGAGTGT GAAGCCGACA AGCAGGCAGC TCTCACTCAG 
CTTTCCCAGC AGAAGGAGCT GGAGCTGATG GCCTCAAGAG AAGGCTGGCA GAGGAAAGTG 
GAAGACCTGC TGGAACAGAT ATCGCTCCTG AAGCAGTCCC TGGAGCTGCA GTTGTCTCAG 
TCTCAGTCTG CTCTCCAGCA GCTGCAGTCT CAGTTCAACC AGGAGAGGGA GCTGCTGAGC 
CAGCAGCTTA AAGAGATGCA GAGGGAACAC CAGAGGAGAG AGCATCGATT ACAGGAGGCT 
CACTGCTGCG CCCTCAGCAC CATGGAGGAG GCCAGACAGC ACCAGATCAG AGCCCTGGAG 
GAGCGTCTGA AGCAGGAGCA CAGGGAGGAG GTCCATGCTC TGAAGGAGGC CCACAGGAGG 
ACCTTGGACA TCCTCAGGCA GCAGTCAGAC CAGGAGCTGC AGACGCTCCG ATTTGAGCTG 
GAGGACGAGG GGAAAGCAAA AATGGCGTCT CTCCGGGCAG AGCTGAACCA CCTCCACGCT 
ACGGCCATCG AGCATTTGAA GCAGATCCAC CTCAAAGAAA ACAGCGCAGC TAAAAGGGAA 
CTGGAGAAGG CGATGGAGCA CAGCCACCAG CAGGAACAAG AACTCCTGGT TCGCATCTCC 
GACCTTCAGG CGGACTTGTG TTCCCGCGGC AACCGCATCA CAGACCTGGA CCACGAAATC 
CACTCTCTGA ACGAGACCAT CGACACGCTG ACCAGAGAGC TGGAGCTGAA GGGCAAAGAG 
GTGCTGCGAG TCCGCAGCGA AGCCAACCAT CAGATAAGGG CTCATGAGCA AGACCTCATA 
AAGAGGCACG AGAGGGACCT GGACGAGATG AAAGTAGTTC ACCACAGAGA AGTGCAGATC 
ATGCTGGCCG ACTTCAACAA GGCCCAGGAG GTGCTCAAAG ACAAGATTTC CGCTCTGCAG 
ATCCTGTTGG AGGGTACGGA GGAGAAGTTG AGGAACAGAG AAAGCCGACC AGAAGATCTC 
CACCTCATAG CAGAGCTCAG AGAGATGGTG ACTGAAAGAG AAGCTCTGGT CAAAAAGCTG 
GTCGACGACA AGAAGTTCTA CCAGCTGGAG CTGGTGAACA GAGAGACGGG CTTCAACAAG 
GTCTTCAATT CCAGTGCCAA CGTTGGTGTC ATCAACCCTC TTATCAAGTC AAAAACAGGC 
AGCCAATCAG ATCAGCGCCT CACCAGCTTT CCTAGCCATT CTGCTCTCCG ATTCGTCAGC 
CCTCCCACCA TGAGCCACGA AGGCCAGGAA GAGGGGGGGC GGGAGGCGGA GGAGGTAGGA 
CCGGGAGGCC GCTATGCGCT TCCTCACTCC CCCCTCCAGA GGTCCCTCCC AAGGTTTAAG 
AAAGCCCTGC CACTGTTCAG CCCTCTTCCT CCTCCTCCTT CTTCTTCTAC TCCTCTACCT 
CAGCATCCTC CTCCTCCTCA TCCCCCACAC CATCCCATGC CGAGCCTCGC TCCTCCTCAT 
GCTTCTCAGG ATCCTCTCGT GCAGCATCAG GGCCTGCAGC GCTGTGGGAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105921453 ( XM_012857170.2 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_012712624.1 Fundulus heteroclitus protein FAM184A (LOC105921453), transcript variant X2, mRNA.

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CloneID	OFu105779
Clone ID Related Accession (Same CDS sequence)	XM_012857170.1 , XM_012857170.2
Accession Version	XM_012857170.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-31
Organism	Fundulus heteroclitus(mummichog)
Product	protein FAM184A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012225189.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 1, 2017 this sequence version replaced XM_012857170.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACGG GGGCAGGCTG GCAGCCTCCG TACAGCACCT CGACGAGCAC CAGCAAATAC 
GCCCCCTCTC CAACGGCGTC CAACATGTTT TACGACGGGA GCTTGACGCT GGAATACACT 
CCGGACCTGC ACCTGAAGAT GAGCAAGAAG ATCGCGCAGC TGACAAAGGT AATTTATGCT 
CTCAACACCA AGAACGATGA GCACGAGGAG GAAATCGAGT CTCTGAAAGA AGCCCACGAG 
GATGAGGTCC AACACATAGT GACGGAAACC AGAGACAAGA TCATGCAGTA CAGGAGCAAA 
ATGGCGGACG AGGCGGACCT GAGGCGGCGG CTGGCCAGTC TGGAGGAATC TGTCGAACTC 
CACGACCACA TGAAGAGACA GGCCTTGGCA GAGTTCGAGA TGTACAGACA GAGGATGGAG 
GACTCGCAGC TCTGCACAGA GGCCCAGCAT ACCCAGAGGG TGGTGTCCAT GAGCAGAGAG 
GTGGAGGCTT TGATGGAGAG GCTGGAGGAG CTGACGAAGG ATAAGGTCCG TCTGGTGGAG 
GAGTACGAGG CCAAGCTGGC CAAGGCGCAG GAGTACTATG AGAGGGAGCT GGAGGCCATG 
AGGAGGACCC ACCAGCTGAC CACTGAGAAC CTGCTGGCCT GGAAGAGGAC GGAGGTGGAG 
CTGCGGAAGG AGTTCCAGGC TCAGGAGGCG GCGCTGCAGC GTTCGCTGTC CAAGCTAAGG 
AGCGAGCTCC AGAAAGCTCA GGAGGAGGCC CGGGAGAACC GCGACAAAAC CAACAGGCTG 
CAGACGTCGC TGGCCAACGC AGAGGGAACC ATCAAGAACC TGCACAAGCA GCTGGAGGAA 
GCCATCCAGG ACGGAGAGAT CTGGGTGATG CAGCTGAAGG ACACCGAGTA TGAACTGGAG 
AGCAGCAGGG AGCGCGTTCA GCAGCAGGCT ACAGAAATCC TCCACAAAGC CAGTCAGATC 
GGCTCCTTGC AGGCCACCCA GATGGCCCAC GAGGCGACCA TCAGGAACCT GGACCAGGAG 
CAGAGTCGTC TCAAGGAGAA GATCGGCCGG CTGGAGGAGG AGCGGGAGGC CCTGCTCAAC 
CAGAGCCAGG CCGCCGGCGA GCAGCACAAA CAGCAGGTGG TGAAACTGGA GCAGTCTCTA 
CGGGAGGAGC ACCAGGGCTA CGAGAAGGAG CTGTCCAGGC TGAGAGCTCA CTACGAGGAG 
GAGACGCTGC GCTTTAAGGA GGCCCAGGTC AGAGCGCTGG AGGAGCTGGA GGAGAAACAC 
AGGGCCATGA GGGAGGAGGC CCAGCAGGAG AAGGAGGATG AGAAGAAGCT CCTCGTCACG 
AAAATGAGTC AGGAGTTTGA GATCAAGCGT CTGTCTTTAG AGGAGCAGAG AGATCGTCTT 
CAGCAGCAGC TGGACAACTT AAAGGAGGAG CTTGCGGCCA AAGTCAACAT GGCCAATCAG 
GAGGTCTCCC ACCTGCAGGA GCTGGTGAGG GAGGGGGAGC AGAACCTGAG CTCAGCTCAG 
ACGCAGATAA GCTGTCTGAA GGAAACCCAG GAGAAGCTGC GCATAGAGCT GGACGCGACC 
AGAGGCCGAG TGAGGGAGAC CAGCAACCTG CTCACTGACC TACAGGAGGA GATCGAGACC 
CAGAAGCAGC AGCATGATGC CAGAGTGATG GCCATCCGGA CGGAGGAGAA GCAGAAGATG 
GACAAGATGG CAGATGACTT GGATCAGAAG TGGAGAGACG CACTGCGGGA GGAAGTGCGT 
TTGCTGAAGG AGGAGCTGAG CGAGGAGTGT GAAGCCGACA AGCAGGCAGC TCTCACTCAG 
CTTTCCCAGC AGAAGGAGCT GGAGCTGATG GCCTCAAGAG AAGGCTGGCA GAGGAAAGTG 
GAAGACCTGC TGGAACAGAT ATCGCTCCTG AAGCAGTCCC TGGAGCTGCA GTTGTCTCAG 
TCTCAGTCTG CTCTCCAGCA GCTGCAGTCT CAGTTCAACC AGGAGAGGGA GCTGCTGAGC 
CAGCAGCTTA AAGAGATGCA GAGGGAACAC CAGAGGAGAG AGCATCGATT ACAGGAGGCT 
CACTGCTGCG CCCTCAGCAC CATGGAGGAG GCCAGACAGC ACCAGATCAG AGCCCTGGAG 
GAGCGTCTGA AGCAGGAGCA CAGGGAGGAG GTCCATGCTC TGAAGGAGGC CCACAGGAGG 
ACCTTGGACA TCCTCAGGCA GCAGTCAGAC CAGGAGCTGC AGACGCTCCG ATTTGAGCTG 
GAGGACGAGG GGAAAGCAAA AATGGCGTCT CTCCGGGCAG AGCTGAACCA CCTCCACGCT 
ACGGCCATCG AGCATTTGAA GCAGATCCAC CTCAAAGAAA ACAGCGCAGC TAAAAGGGAA 
CTGGAGAAGG CGATGGAGCA CAGCCACCAG CAGGAACAAG AACTCCTGGT TCGCATCTCC 
GACCTTCAGG CGGACTTGTG TTCCCGCGGC AACCGCATCA CAGACCTGGA CCACGAAATC 
CACTCTCTGA ACGAGACCAT CGACACGCTG ACCAGAGAGC TGGAGCTGAA GGGCAAAGAG 
GTGCTGCGAG TCCGCAGCGA AGCCAACCAT CAGATAAGGG CTCATGAGCA AGACCTCATA 
AAGAGGCACG AGAGGGACCT GGACGAGATG AAAGTAGTTC ACCACAGAGA AGTGCAGATC 
ATGCTGGCCG ACTTCAACAA GGCCCAGGAG GTGCTCAAAG ACAAGATTTC CGCTCTGCAG 
ATCCTGTTGG AGGGTACGGA GGAGAAGTTG AGGAACAGAG AAAGCCGACC AGAAGATCTC 
CACCTCATAG CAGAGCTCAG AGAGATGGTG ACTGAAAGAG AAGCTCTGGT CAAAAAGCTG 
GTCGACGACA AGAAGTTCTA CCAGCTGGAG CTGGTGAACA GAGAGACGGG CTTCAACAAG 
GTCTTCAATT CCAGTGCCAA CGTTGGTGTC ATCAACCCTC TTATCAAGTC AAAAACAGGC 
AGCCAATCAG ATCAGCGCCT CACCAGCTTT CCTAGCCATT CTGCTCTCCG ATTCGTCAGC 
CCTCCCACCA TGAGCCACGA AGGCCAGGAA GAGGGGGGGC GGGAGGCGGA GGAGGTAGGA 
CCGGGAGGCC GCTATGCGCT TCCTCACTCC CCCCTCCAGA GGTCCCTCCC AAGGTTTAAG 
AAAGCCCTGC CACTGTTCAG CCCTCTTCCT CCTCCTCCTT CTTCTTCTAC TCCTCTACCT 
CAGCATCCTC CTCCTCCTCA TCCCCCACAC CATCCCATGC CGAGCCTCGC TCCTCCTCAT 
GCTTCTCAGG ATCCTCTCGT GCAGCATCAG GGCCTGCAGC GCTGTGGGAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012712624.1
CDS	350..3643
Translation	MATGAGWQPPYSTSTSTSKYAPSPTASNMFYDGSLTLEYTPDLHLKMSKKIAQLTKVIYALNTKNDEHEEEIESLKEAHEDEVQHIVTETRDKIMQYRSKMADEADLRRRLASLEESVELHDHMKRQALAEFEMYRQRMEDSQLCTEAQHTQRVVSMSREVEALMERLEELTKDKVRLVEEYEAKLAKAQEYYERELEAMRRTHQLTTENLLAWKRTEVELRKEFQAQEAALQRSLSKLRSELQKAQEEARENRDKTNRLQTSLANAEGTIKNLHKQLEEAIQDGEIWVMQLKDTEYELESSRERVQQQATEILHKASQIGSLQATQMAHEATIRNLDQEQSRLKEKIGRLEEEREALLNQSQAAGEQHKQQVVKLEQSLREEHQGYEKELSRLRAHYEEETLRFKEAQVRALEELEEKHRAMREEAQQEKEDEKKLLVTKMSQEFEIKRLSLEEQRDRLQQQLDNLKEELAAKVNMANQEVSHLQELVREGEQNLSSAQTQISCLKETQEKLRIELDATRGRVRETSNLLTDLQEEIETQKQQHDARVMAIRTEEKQKMDKMADDLDQKWRDALREEVRLLKEELSEECEADKQAALTQLSQQKELELMASREGWQRKVEDLLEQISLLKQSLELQLSQSQSALQQLQSQFNQERELLSQQLKEMQREHQRREHRLQEAHCCALSTMEEARQHQIRALEERLKQEHREEVHALKEAHRRTLDILRQQSDQELQTLRFELEDEGKAKMASLRAELNHLHATAIEHLKQIHLKENSAAKRELEKAMEHSHQQEQELLVRISDLQADLCSRGNRITDLDHEIHSLNETIDTLTRELELKGKEVLRVRSEANHQIRAHEQDLIKRHERDLDEMKVVHHREVQIMLADFNKAQEVLKDKISALQILLEGTEEKLRNRESRPEDLHLIAELREMVTEREALVKKLVDDKKFYQLELVNRETGFNKVFNSSANVGVINPLIKSKTGSQSDQRLTSFPSHSALRFVSPPTMSHEGQEEGGREAEEVGPGGRYALPHSPLQRSLPRFKKALPLFSPLPPPPSSSTPLPQHPPPPHPPHHPMPSLAPPHASQDPLVQHQGLQRCGR

Target ORF information:

RefSeq Version	XM_012857170.2
Organism	Fundulus heteroclitus(mummichog)
Definition	Fundulus heteroclitus protein FAM184A (LOC105921453), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012857170.2

ORF Insert Sequence:

ATGGCGACGG GGGCAGGCTG GCAGCCTCCG TACAGCACCT CGACGAGCAC CAGCAAATAC 
GCCCCCTCTC CAACGGCGTC CAACATGTTT TACGACGGGA GCTTGACGCT GGAATACACT 
CCGGACCTGC ACCTGAAGAT GAGCAAGAAG ATCGCGCAGC TGACAAAGGT AATTTATGCT 
CTCAACACCA AGAACGATGA GCACGAGGAG GAAATCGAGT CTCTGAAAGA AGCCCACGAG 
GATGAGGTCC AACACATAGT GACGGAAACC AGAGACAAGA TCATGCAGTA CAGGAGCAAA 
ATGGCGGACG AGGCGGACCT GAGGCGGCGG CTGGCCAGTC TGGAGGAATC TGTCGAACTC 
CACGACCACA TGAAGAGACA GGCCTTGGCA GAGTTCGAGA TGTACAGACA GAGGATGGAG 
GACTCGCAGC TCTGCACAGA GGCCCAGCAT ACCCAGAGGG TGGTGTCCAT GAGCAGAGAG 
GTGGAGGCTT TGATGGAGAG GCTGGAGGAG CTGACGAAGG ATAAGGTCCG TCTGGTGGAG 
GAGTACGAGG CCAAGCTGGC CAAGGCGCAG GAGTACTATG AGAGGGAGCT GGAGGCCATG 
AGGAGGACCC ACCAGCTGAC CACTGAGAAC CTGCTGGCCT GGAAGAGGAC GGAGGTGGAG 
CTGCGGAAGG AGTTCCAGGC TCAGGAGGCG GCGCTGCAGC GTTCGCTGTC CAAGCTAAGG 
AGCGAGCTCC AGAAAGCTCA GGAGGAGGCC CGGGAGAACC GCGACAAAAC CAACAGGCTG 
CAGACGTCGC TGGCCAACGC AGAGGGAACC ATCAAGAACC TGCACAAGCA GCTGGAGGAA 
GCCATCCAGG ACGGAGAGAT CTGGGTGATG CAGCTGAAGG ACACCGAGTA TGAACTGGAG 
AGCAGCAGGG AGCGCGTTCA GCAGCAGGCT ACAGAAATCC TCCACAAAGC CAGTCAGATC 
GGCTCCTTGC AGGCCACCCA GATGGCCCAC GAGGCGACCA TCAGGAACCT GGACCAGGAG 
CAGAGTCGTC TCAAGGAGAA GATCGGCCGG CTGGAGGAGG AGCGGGAGGC CCTGCTCAAC 
CAGAGCCAGG CCGCCGGCGA GCAGCACAAA CAGCAGGTGG TGAAACTGGA GCAGTCTCTA 
CGGGAGGAGC ACCAGGGCTA CGAGAAGGAG CTGTCCAGGC TGAGAGCTCA CTACGAGGAG 
GAGACGCTGC GCTTTAAGGA GGCCCAGGTC AGAGCGCTGG AGGAGCTGGA GGAGAAACAC 
AGGGCCATGA GGGAGGAGGC CCAGCAGGAG AAGGAGGATG AGAAGAAGCT CCTCGTCACG 
AAAATGAGTC AGGAGTTTGA GATCAAGCGT CTGTCTTTAG AGGAGCAGAG AGATCGTCTT 
CAGCAGCAGC TGGACAACTT AAAGGAGGAG CTTGCGGCCA AAGTCAACAT GGCCAATCAG 
GAGGTCTCCC ACCTGCAGGA GCTGGTGAGG GAGGGGGAGC AGAACCTGAG CTCAGCTCAG 
ACGCAGATAA GCTGTCTGAA GGAAACCCAG GAGAAGCTGC GCATAGAGCT GGACGCGACC 
AGAGGCCGAG TGAGGGAGAC CAGCAACCTG CTCACTGACC TACAGGAGGA GATCGAGACC 
CAGAAGCAGC AGCATGATGC CAGAGTGATG GCCATCCGGA CGGAGGAGAA GCAGAAGATG 
GACAAGATGG CAGATGACTT GGATCAGAAG TGGAGAGACG CACTGCGGGA GGAAGTGCGT 
TTGCTGAAGG AGGAGCTGAG CGAGGAGTGT GAAGCCGACA AGCAGGCAGC TCTCACTCAG 
CTTTCCCAGC AGAAGGAGCT GGAGCTGATG GCCTCAAGAG AAGGCTGGCA GAGGAAAGTG 
GAAGACCTGC TGGAACAGAT ATCGCTCCTG AAGCAGTCCC TGGAGCTGCA GTTGTCTCAG 
TCTCAGTCTG CTCTCCAGCA GCTGCAGTCT CAGTTCAACC AGGAGAGGGA GCTGCTGAGC 
CAGCAGCTTA AAGAGATGCA GAGGGAACAC CAGAGGAGAG AGCATCGATT ACAGGAGGCT 
CACTGCTGCG CCCTCAGCAC CATGGAGGAG GCCAGACAGC ACCAGATCAG AGCCCTGGAG 
GAGCGTCTGA AGCAGGAGCA CAGGGAGGAG GTCCATGCTC TGAAGGAGGC CCACAGGAGG 
ACCTTGGACA TCCTCAGGCA GCAGTCAGAC CAGGAGCTGC AGACGCTCCG ATTTGAGCTG 
GAGGACGAGG GGAAAGCAAA AATGGCGTCT CTCCGGGCAG AGCTGAACCA CCTCCACGCT 
ACGGCCATCG AGCATTTGAA GCAGATCCAC CTCAAAGAAA ACAGCGCAGC TAAAAGGGAA 
CTGGAGAAGG CGATGGAGCA CAGCCACCAG CAGGAACAAG AACTCCTGGT TCGCATCTCC 
GACCTTCAGG CGGACTTGTG TTCCCGCGGC AACCGCATCA CAGACCTGGA CCACGAAATC 
CACTCTCTGA ACGAGACCAT CGACACGCTG ACCAGAGAGC TGGAGCTGAA GGGCAAAGAG 
GTGCTGCGAG TCCGCAGCGA AGCCAACCAT CAGATAAGGG CTCATGAGCA AGACCTCATA 
AAGAGGCACG AGAGGGACCT GGACGAGATG AAAGTAGTTC ACCACAGAGA AGTGCAGATC 
ATGCTGGCCG ACTTCAACAA GGCCCAGGAG GTGCTCAAAG ACAAGATTTC CGCTCTGCAG 
ATCCTGTTGG AGGGTACGGA GGAGAAGTTG AGGAACAGAG AAAGCCGACC AGAAGATCTC 
CACCTCATAG CAGAGCTCAG AGAGATGGTG ACTGAAAGAG AAGCTCTGGT CAAAAAGCTG 
GTCGACGACA AGAAGTTCTA CCAGCTGGAG CTGGTGAACA GAGAGACGGG CTTCAACAAG 
GTCTTCAATT CCAGTGCCAA CGTTGGTGTC ATCAACCCTC TTATCAAGTC AAAAACAGGC 
AGCCAATCAG ATCAGCGCCT CACCAGCTTT CCTAGCCATT CTGCTCTCCG ATTCGTCAGC 
CCTCCCACCA TGAGCCACGA AGGCCAGGAA GAGGGGGGGC GGGAGGCGGA GGAGGTAGGA 
CCGGGAGGCC GCTATGCGCT TCCTCACTCC CCCCTCCAGA GGTCCCTCCC AAGGTTTAAG 
AAAGCCCTGC CACTGTTCAG CCCTCTTCCT CCTCCTCCTT CTTCTTCTAC TCCTCTACCT 
CAGCATCCTC CTCCTCCTCA TCCCCCACAC CATCCCATGC CGAGCCTCGC TCCTCCTCAT 
GCTTCTCAGG ATCCTCTCGT GCAGCATCAG GGCCTGCAGC GCTGTGGGAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105921453 ( XM_012857172.1 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_012712626.1 Fundulus heteroclitus family with sequence similarity 184, member A (fam184a), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OFu105781
Clone ID Related Accession (Same CDS sequence)	XM_012857172.1
Accession Version	XM_012857172.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-14
Organism	Fundulus heteroclitus(Mummichog)
Product	protein FAM184A isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012225189.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTACA GGAGCAAAAT GGCGGACGAG GCGGACCTGA GGCGGCGGCT GGCCAGTCTG 
GAGGAATCTG TCGAACTCCA CGACCACATG AAGAGACAGG CCTTGGCAGA GTTCGAGATG 
TACAGACAGA GGATGGAGGA CTCGCAGCTC TGCACAGAGG CCCAGCATAC CCAGAGGGTG 
GTGTCCATGA GCAGAGAGGT GGAAGAGATG CGTCGCGATT TTGAGGAGAA GCTTCGGGTG 
TTCAGCCAGG CTCAGGCCCA GTTTGAGGCA GATAAGCGGC GGGCGCTGGA CGAGCTGAGG 
GCCACCCACC GACACGAGGT GGAGGAGCTC CGCAACAACC AGCAGAACCA CAGCGCCACC 
TCTGCCGAGG ACCAGGAGAA ACTGGCCGAG CTGCATAGAC AAGAGGTGGA GGCTTTGATG 
GAGAGGCTGG AGGAGCTGAC GAAGGATAAG GTCCGTCTGG TGGAGGAGTA CGAGGCCAAG 
CTGGCCAAGG CGCAGGAGTA CTATGAGAGG GAGCTGGAGG CCATGAGGAG GACCCACCAG 
CTGACCACTG AGAACCTGCT GGCCTGGAAG AGGACGGAGG TGGAGCTGCG GAAGGAGTTC 
CAGGCTCAGG AGGCGGCGCT GCAGCGTTCG CTGTCCAAGC TAAGGAGCGA GCTCCAGAAA 
GCTCAGGAGG AGGCCCGGGA GAACCGCGAC AAAACCAACA GGCTGCAGAC GTCGCTGGCC 
AACGCAGAGG GAACCATCAA GAACCTGCAC AAGCAGCTGG AGGAAGCCAT CCAGGACGGA 
GAGATCTGGG TGATGCAGCT GAAGGACACC GAGTATGAAC TGGAGAGCAG CAGGGAGCGC 
GTTCAGCAGC AGGCTACAGA AATCCTCCAC AAAGCCAGTC AGATCGGCTC CTTGCAGGCC 
ACCCAGATGG CCCACGAGGC GACCATCAGG AACCTGGACC AGGAGCAGAG TCGTCTCAAG 
GAGAAGATCG GCCGGCTGGA GGAGGAGCGG GAGGCCCTGC TCAACCAGAG CCAGGCCGCC 
GGCGAGCAGC ACAAACAGCA GGTGGTGAAA CTGGAGCAGT CTCTACGGGA GGAGCACCAG 
GGCTACGAGA AGGAGCTGTC CAGGCTGAGA GCTCACTACG AGGAGGAGAC GCTGCGCTTT 
AAGGAGGCCC AGGTCAGAGC GCTGGAGGAG CTGGAGGAGA AACACAGGGC CATGAGGGAG 
GAGGCCCAGC AGGAGAAGGA GGATGAGAAG AAGCTCCTCG TCACGAAAAT GAGTCAGGAG 
TTTGAGATCA AGCGTCTGTC TTTAGAGGAG CAGAGAGATC GTCTTCAGCA GCAGCTGGAC 
AACTTAAAGG AGGAGCTTGC GGCCAAAGTC AACATGGCCA ATCAGGAGGT CTCCCACCTG 
CAGGAGCTGG TGAGGGAGGG GGAGCAGAAC CTGAGCTCAG CTCAGACGCA GATAAGCTGT 
CTGAAGGAAA CCCAGGAGAA GCTGCGCATA GAGCTGGACG CGACCAGAGG CCGAGTGAGG 
GAGACCAGCA ACCTGCTCAC TGACCTACAG GAGGAGATCG AGACCCAGAA GCAGCAGCAT 
GATGCCAGAG TGATGGCCAT CCGGACGGAG GAGAAGCAGA AGATGGACAA GATGGCAGAT 
GACTTGGATC AGAAGTGGAG AGACGCACTG CGGGAGGAAG TGCGTTTGCT GAAGGAGGAG 
CTGAGCGAGG AGTGTGAAGC CGACAAGCAG GCAGCTCTCA CTCAGCTTTC CCAGCAGAAG 
GAGCTGGAGC TGATGGCCTC AAGAGAAGGC TGGCAGAGGA AAGTGGAAGA CCTGCTGGAA 
CAGATATCGC TCCTGAAGCA GTCCCTGGAG CTGCAGTTGT CTCAGTCTCA GTCTGCTCTC 
CAGCAGCTGC AGTCTCAGTT CAACCAGGAG AGGGAGCTGC TGAGCCAGCA GCTTAAAGAG 
ATGCAGAGGG AACACCAGAG GAGAGAGCAT CGATTACAGG AGGCTCACTG CTGCGCCCTC 
AGCACCATGG AGGAGGCCAG ACAGCACCAG ATCAGAGCCC TGGAGGAGCG TCTGAAGCAG 
GAGCACAGGG AGGAGGTCCA TGCTCTGAAG GAGGCCCACA GGAGGACCTT GGACATCCTC 
AGGCAGCAGT CAGACCAGGA GCTGCAGACG CTCCGATTTG AGCTGGAGGA CGAGGGGAAA 
GCAAAAATGG CGTCTCTCCG GGCAGAGCTG AACCACCTCC ACGCTACGGC CATCGAGCAT 
TTGAAGCAGA TCCACCTCAA AGAAAACAGC GCAGCTAAAA GGGAACTGGA GAAGGCGATG 
GAGCACAGCC ACCAGCAGGA ACAAGAACTC CTGGTTCGCA TCTCCGACCT TCAGGCGGAC 
TTGTGTTCCC GCGGCAACCG CATCACAGAC CTGGACCACG AAATCCACTC TCTGAACGAG 
ACCATCGACA CGCTGACCAG AGAGCTGGAG CTGAAGGGCA AAGAGGTGCT GCGAGTCCGC 
AGCGAAGCCA ACCATCAGAT AAGGGCTCAT GAGCAAGACC TCATAAAGAG GCACGAGAGG 
GACCTGGACG AGATGAAAGT AGTTCACCAC AGAGAAGTGC AGATCATGCT GGCCGACTTC 
AACAAGGCCC AGGAGGTGCT CAAAGACAAG ATTTCCGCTC TGCAGATCCT GTTGGAGGGT 
ACGGAGGAGA AGTTGAGGAA CAGAGAAAGC CGACCAGAAG ATCTCCACCT CATAGCAGAG 
CTCAGAGAGA TGGTGACTGA AAGAGAAGCT CTGGTCAAAA AGCTGGTCGA CGACAAGAAG 
TTCTACCAGC TGGAGCTGGT GAACAGAGAG ACGGGCTTCA ACAAGGTCTT CAATTCCAGT 
GCCAACGTTG GTGTCATCAA CCCTCTTATC AAGTCAAAAA CAGGCAGCCA ATCAGATCAG 
CGCCTCACCA GCTTTCCTAG CCATTCTGCT CTCCGATTCG TCAGCCCTCC CACCATGAGC 
CACGAAGGCC AGGAAGAGGG GGGGCGGGAG GCGGAGGAGG TAGGACCGGG AGGCCGCTAT 
GCGCTTCCTC ACTCCCCCCT CCAGAGGTCC CTCCCAAGGT TTAAGAAAGC CCTGCCACTG 
TTCAGCCCTC TTCCTCCTCC TCCTTCTTCT TCTACTCCTC TACCTCAGCA TCCTCCTCCT 
CCTCATCCCC CACACCATCC CATGCCGAGC CTCGCTCCTC CTCATGCTTC TCAGGATCCT 
CTCGTGCAGC ATCAGGGCCT GCAGCGCTGT GGGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012712626.1
CDS	200..3418
Translation	MQYRSKMADEADLRRRLASLEESVELHDHMKRQALAEFEMYRQRMEDSQLCTEAQHTQRVVSMSREVEEMRRDFEEKLRVFSQAQAQFEADKRRALDELRATHRHEVEELRNNQQNHSATSAEDQEKLAELHRQEVEALMERLEELTKDKVRLVEEYEAKLAKAQEYYERELEAMRRTHQLTTENLLAWKRTEVELRKEFQAQEAALQRSLSKLRSELQKAQEEARENRDKTNRLQTSLANAEGTIKNLHKQLEEAIQDGEIWVMQLKDTEYELESSRERVQQQATEILHKASQIGSLQATQMAHEATIRNLDQEQSRLKEKIGRLEEEREALLNQSQAAGEQHKQQVVKLEQSLREEHQGYEKELSRLRAHYEEETLRFKEAQVRALEELEEKHRAMREEAQQEKEDEKKLLVTKMSQEFEIKRLSLEEQRDRLQQQLDNLKEELAAKVNMANQEVSHLQELVREGEQNLSSAQTQISCLKETQEKLRIELDATRGRVRETSNLLTDLQEEIETQKQQHDARVMAIRTEEKQKMDKMADDLDQKWRDALREEVRLLKEELSEECEADKQAALTQLSQQKELELMASREGWQRKVEDLLEQISLLKQSLELQLSQSQSALQQLQSQFNQERELLSQQLKEMQREHQRREHRLQEAHCCALSTMEEARQHQIRALEERLKQEHREEVHALKEAHRRTLDILRQQSDQELQTLRFELEDEGKAKMASLRAELNHLHATAIEHLKQIHLKENSAAKRELEKAMEHSHQQEQELLVRISDLQADLCSRGNRITDLDHEIHSLNETIDTLTRELELKGKEVLRVRSEANHQIRAHEQDLIKRHERDLDEMKVVHHREVQIMLADFNKAQEVLKDKISALQILLEGTEEKLRNRESRPEDLHLIAELREMVTEREALVKKLVDDKKFYQLELVNRETGFNKVFNSSANVGVINPLIKSKTGSQSDQRLTSFPSHSALRFVSPPTMSHEGQEEGGREAEEVGPGGRYALPHSPLQRSLPRFKKALPLFSPLPPPPSSSTPLPQHPPPPHPPHHPMPSLAPPHASQDPLVQHQGLQRCGR

Target ORF information:

RefSeq Version	XM_012857172.1
Organism	Fundulus heteroclitus(Mummichog)
Definition	Fundulus heteroclitus family with sequence similarity 184, member A (fam184a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012857172.1

ORF Insert Sequence:

ATGCAGTACA GGAGCAAAAT GGCGGACGAG GCGGACCTGA GGCGGCGGCT GGCCAGTCTG 
GAGGAATCTG TCGAACTCCA CGACCACATG AAGAGACAGG CCTTGGCAGA GTTCGAGATG 
TACAGACAGA GGATGGAGGA CTCGCAGCTC TGCACAGAGG CCCAGCATAC CCAGAGGGTG 
GTGTCCATGA GCAGAGAGGT GGAAGAGATG CGTCGCGATT TTGAGGAGAA GCTTCGGGTG 
TTCAGCCAGG CTCAGGCCCA GTTTGAGGCA GATAAGCGGC GGGCGCTGGA CGAGCTGAGG 
GCCACCCACC GACACGAGGT GGAGGAGCTC CGCAACAACC AGCAGAACCA CAGCGCCACC 
TCTGCCGAGG ACCAGGAGAA ACTGGCCGAG CTGCATAGAC AAGAGGTGGA GGCTTTGATG 
GAGAGGCTGG AGGAGCTGAC GAAGGATAAG GTCCGTCTGG TGGAGGAGTA CGAGGCCAAG 
CTGGCCAAGG CGCAGGAGTA CTATGAGAGG GAGCTGGAGG CCATGAGGAG GACCCACCAG 
CTGACCACTG AGAACCTGCT GGCCTGGAAG AGGACGGAGG TGGAGCTGCG GAAGGAGTTC 
CAGGCTCAGG AGGCGGCGCT GCAGCGTTCG CTGTCCAAGC TAAGGAGCGA GCTCCAGAAA 
GCTCAGGAGG AGGCCCGGGA GAACCGCGAC AAAACCAACA GGCTGCAGAC GTCGCTGGCC 
AACGCAGAGG GAACCATCAA GAACCTGCAC AAGCAGCTGG AGGAAGCCAT CCAGGACGGA 
GAGATCTGGG TGATGCAGCT GAAGGACACC GAGTATGAAC TGGAGAGCAG CAGGGAGCGC 
GTTCAGCAGC AGGCTACAGA AATCCTCCAC AAAGCCAGTC AGATCGGCTC CTTGCAGGCC 
ACCCAGATGG CCCACGAGGC GACCATCAGG AACCTGGACC AGGAGCAGAG TCGTCTCAAG 
GAGAAGATCG GCCGGCTGGA GGAGGAGCGG GAGGCCCTGC TCAACCAGAG CCAGGCCGCC 
GGCGAGCAGC ACAAACAGCA GGTGGTGAAA CTGGAGCAGT CTCTACGGGA GGAGCACCAG 
GGCTACGAGA AGGAGCTGTC CAGGCTGAGA GCTCACTACG AGGAGGAGAC GCTGCGCTTT 
AAGGAGGCCC AGGTCAGAGC GCTGGAGGAG CTGGAGGAGA AACACAGGGC CATGAGGGAG 
GAGGCCCAGC AGGAGAAGGA GGATGAGAAG AAGCTCCTCG TCACGAAAAT GAGTCAGGAG 
TTTGAGATCA AGCGTCTGTC TTTAGAGGAG CAGAGAGATC GTCTTCAGCA GCAGCTGGAC 
AACTTAAAGG AGGAGCTTGC GGCCAAAGTC AACATGGCCA ATCAGGAGGT CTCCCACCTG 
CAGGAGCTGG TGAGGGAGGG GGAGCAGAAC CTGAGCTCAG CTCAGACGCA GATAAGCTGT 
CTGAAGGAAA CCCAGGAGAA GCTGCGCATA GAGCTGGACG CGACCAGAGG CCGAGTGAGG 
GAGACCAGCA ACCTGCTCAC TGACCTACAG GAGGAGATCG AGACCCAGAA GCAGCAGCAT 
GATGCCAGAG TGATGGCCAT CCGGACGGAG GAGAAGCAGA AGATGGACAA GATGGCAGAT 
GACTTGGATC AGAAGTGGAG AGACGCACTG CGGGAGGAAG TGCGTTTGCT GAAGGAGGAG 
CTGAGCGAGG AGTGTGAAGC CGACAAGCAG GCAGCTCTCA CTCAGCTTTC CCAGCAGAAG 
GAGCTGGAGC TGATGGCCTC AAGAGAAGGC TGGCAGAGGA AAGTGGAAGA CCTGCTGGAA 
CAGATATCGC TCCTGAAGCA GTCCCTGGAG CTGCAGTTGT CTCAGTCTCA GTCTGCTCTC 
CAGCAGCTGC AGTCTCAGTT CAACCAGGAG AGGGAGCTGC TGAGCCAGCA GCTTAAAGAG 
ATGCAGAGGG AACACCAGAG GAGAGAGCAT CGATTACAGG AGGCTCACTG CTGCGCCCTC 
AGCACCATGG AGGAGGCCAG ACAGCACCAG ATCAGAGCCC TGGAGGAGCG TCTGAAGCAG 
GAGCACAGGG AGGAGGTCCA TGCTCTGAAG GAGGCCCACA GGAGGACCTT GGACATCCTC 
AGGCAGCAGT CAGACCAGGA GCTGCAGACG CTCCGATTTG AGCTGGAGGA CGAGGGGAAA 
GCAAAAATGG CGTCTCTCCG GGCAGAGCTG AACCACCTCC ACGCTACGGC CATCGAGCAT 
TTGAAGCAGA TCCACCTCAA AGAAAACAGC GCAGCTAAAA GGGAACTGGA GAAGGCGATG 
GAGCACAGCC ACCAGCAGGA ACAAGAACTC CTGGTTCGCA TCTCCGACCT TCAGGCGGAC 
TTGTGTTCCC GCGGCAACCG CATCACAGAC CTGGACCACG AAATCCACTC TCTGAACGAG 
ACCATCGACA CGCTGACCAG AGAGCTGGAG CTGAAGGGCA AAGAGGTGCT GCGAGTCCGC 
AGCGAAGCCA ACCATCAGAT AAGGGCTCAT GAGCAAGACC TCATAAAGAG GCACGAGAGG 
GACCTGGACG AGATGAAAGT AGTTCACCAC AGAGAAGTGC AGATCATGCT GGCCGACTTC 
AACAAGGCCC AGGAGGTGCT CAAAGACAAG ATTTCCGCTC TGCAGATCCT GTTGGAGGGT 
ACGGAGGAGA AGTTGAGGAA CAGAGAAAGC CGACCAGAAG ATCTCCACCT CATAGCAGAG 
CTCAGAGAGA TGGTGACTGA AAGAGAAGCT CTGGTCAAAA AGCTGGTCGA CGACAAGAAG 
TTCTACCAGC TGGAGCTGGT GAACAGAGAG ACGGGCTTCA ACAAGGTCTT CAATTCCAGT 
GCCAACGTTG GTGTCATCAA CCCTCTTATC AAGTCAAAAA CAGGCAGCCA ATCAGATCAG 
CGCCTCACCA GCTTTCCTAG CCATTCTGCT CTCCGATTCG TCAGCCCTCC CACCATGAGC 
CACGAAGGCC AGGAAGAGGG GGGGCGGGAG GCGGAGGAGG TAGGACCGGG AGGCCGCTAT 
GCGCTTCCTC ACTCCCCCCT CCAGAGGTCC CTCCCAAGGT TTAAGAAAGC CCTGCCACTG 
TTCAGCCCTC TTCCTCCTCC TCCTTCTTCT TCTACTCCTC TACCTCAGCA TCCTCCTCCT 
CCTCATCCCC CACACCATCC CATGCCGAGC CTCGCTCCTC CTCATGCTTC TCAGGATCCT 
CTCGTGCAGC ATCAGGGCCT GCAGCGCTGT GGGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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見積依頼

Eメール

[email protected]

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

LOC105921453 cDNA ORF clone, Fundulus heteroclitus(mummichog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム