mbd1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mbd1a
Entrez Gene ID	797143
Full Name	methyl-CpG binding domain protein 1a
Synonyms	fc83c09,im:7136442,si:dkey-242k1.6,wu:fc39d12,wu:fc83c09
General protein information	Preferred Names methyl-CpG binding domain protein 1a Names methyl-CpG binding domain protein fc39d12
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009303760.3	XP_009302035.1	methyl-CpG binding domain protein isoform X2
XM_009303760.2	XP_009302035.1	methyl-CpG binding domain protein isoform X2
XM_009303759.2	XP_009302034.1	methyl-CpG binding domain protein isoform X1
NM_001309889.1	NP_001296818.1	methyl-CpG binding domain protein
XM_009303759.3	XP_009302034.1	methyl-CpG binding domain protein isoform X1
XM_021478323.1	XP_021333998.1	methyl-CpG binding domain protein isoform X3

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mbd1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mbd1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa47148	NM_001309889.1 Latest version!	Danio rerio methyl-CpG binding domain protein 1a (mbd1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa47149	XM_009303759.3 Latest version!	Danio rerio methyl-CpG binding domain protein 1a (mbd1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa47150	XM_009303760.3 Latest version!	Danio rerio methyl-CpG binding domain protein 1a (mbd1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa47151	XM_021478323.1 Latest version!	Danio rerio methyl-CpG binding domain protein 1a (mbd1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
ODa47150	XM_009303760.2	Danio rerio methyl-CpG binding domain protein 1a (mbd1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa47149	XM_009303759.2	Danio rerio methyl-CpG binding domain protein 1a (mbd1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mbd1a ( NM_001309889.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001296818.1 Danio rerio methyl-CpG binding domain protein 1a (mbd1a), mRNA.

Price & Availability↑

CloneID	ODa47148
Clone ID Related Accession (Same CDS sequence)	NM_001309889.1
Accession Version	NM_001309889.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2394bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	methyl-CpG binding domain protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR931813.19 and FQ377654.16. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01017340.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168448, SAMEA4476733 [ECO:0000348] ##Evidence-Data-END##

ATGACGGATG AGCTGAACGA GCTATCACCT GAATCTGAGA TCTTGAAACC AGAAGACGCT 
GTGCCAGCAT ACTTAAACGA GATGCATCCG ATCGGAGAAG ACGTTGATAG TGACGTTGAG 
GATGACGACA TGTCCTGCAA AGTGACGGTT GAGTTATTAC CTGATTGGTT GAAGCCTATG 
GAGGAGGAGG AAGTTGGTGT TGATGAGCTC ATGAGCGCTG GACGGATCAA CCGGAGAAAA 
ACTAAAGAAA AACCTCCGAA AGTAAAACGG GGCAGGGGTC GACCTCCTAA AATAAGACAT 
GGACTTAGTC GTTCAGAGTT CAAGAAAGGA CTGCATGCGG ATCGCACTAT GAAGAAAAAG 
AGGGTTGGAT CCCTAATGAG TCGCAAACGT TTATCCCGAA AAGCCACTTC CGTCCCAGAT 
CCATCGCTCA AGGCTGAAGT TTTACCAAGA ACCCGCCAAA GAGTTGTTCG CAGCTCAACA 
TCCACACCTC AAAGTACCAA ACAGGTCCCA GACTCTCCAG CGGGAGGCAC CAGCGCAGAA 
TTTAACAGCC CTCCGCGCCT CACAAGGGTC TCGCCGCCCA GTATCGACAC GTCCAGGCGC 
AGCTTCGTTC ACTCTCCTTC TGTTAAAGTG GGTAACGTCA CCCAGCAGAT AATCAGCACT 
GAACCTGGGA AAGATGTCTC TGAGCTGCCG CTTCAACTTT GGCTTTGTCC TTCTCTGGAG 
CCCATTAGCC CTACACAAAC TCAGGAAATA CTCTCGCAGC AAACCGTAGG GTCACCAAAT 
TTTCCAGAGG CTTCGCTGAA TGGGACAATG ACGGTGAAGG AGAGTCTGAC AATGACGGTG 
AAGGAGAGTC TTCCTTTGCT CAGTGAATGT GAAGGATGCG GATGTCCATT TTCAGCTCAG 
AAGCATGGAG ACACTTTGTG CTACAGATGC AGAATTAAAA GAGAGAAGAA GCGGTCTCCT 
CCAAACATTT TGTGTAGGAA GGTGAGGCAG GATCAGTGGG AGGTTGAGAA AGAAAAACAT 
CCAAGAAAGC AAATGCTGCA GAGACCACAA AGATATGGGA AAACCGAAAA TGATATTGTG 
CCTGATGGTG GTGATGATGA TGATGAAGAG TACTGGCGTT TAAAGAAGCG TAATCGGAGG 
ATGTGTCAGC GGTGTGATGC CTGCCGTCGT GATACAGACT GTGGGAAGTG CGACTTCTGC 
ATGGACAAGC CCAAGTTTGG TGGCAGTAAC AAGAAAAGGC AGAAATGTCG CTTGCGTCAG 
TGCAAGTTTC AGTCAAAGCT TCAATTTCGA AATGGAATGA AGGGAGTGCC GACTTCATCT 
CTTTCACCAA TAAAGAAAAT GATCAAATCC AATAAACTCC TCAAAATAAA GAAACGTGGG 
AGACCACGCA AAAAAAAATT CAAAAATGAC CCATGGGAGG AGGAGGAGGA GGATTATGAA 
GTGTCTGATA ATGATGAAGA TGATCAAGAG TTGAGGAGTT CTGAGATGAA CGGCTCCCAG 
GTCAGAAAAC ACGGGAAATG GAGTTACACT TTTAAGGAAG ATGAAGACGA CGCGGCCTAT 
GTTGAGGATG TGGTTGATGC CAACATTGAA GAGGATTCTG TTATTTTGGA TAATGAACGG 
TCAGTGATGG CGTCTAACGA GATGTACAAC ACCACCTCTG GACTCAGTTC TCAGGGATTA 
TACTACAATG TGTCAGGAAT GCCGCTGTCG TCTCATCTGA TTGGCTCAGC TCCTATCTGT 
AACAGCGGTG CAGTGGCTAT GGGTGAGGTG ATCGGCTCGG TGCCCATGGC GGACGAGATG 
AGCCAGAATG GATTTCTTCA GATCGAGATG GTGAAAGTGG GATCATCTCC ATCACATTAC 
AGAGAAGAAC AGCAGAACAC ACAACAAATG CACATTGCAG ACCCTCAACA GGAGCACACG 
CCGGTGATCA CTCAGATCTT CAGTCTGGCG GGTCCAGAGA CAGAAAGTGA TCGAGACCCA 
GGCTTGATCG AGCTGTTCAC GTCCCTCGGC CAGAGCGTCC TGCCGGCTCA CTGGGTGTGT 
GTGATCGCCA AAGGCCCTGT CCTGCAGCTC CTGCAGTGCT CTAAACTCTC CACCATGGCA 
GACACCGTGG TCCAGATCGA GAAAGGCTTC TTCTACCAGG TCACCGTCCA GAACCAGCCT 
CTGCTGCTCA TGCACAGCCT CTACTCTCGC CATCCCACCT GCCTGGAGAA AGTGGAGCAT 
GTAGTGTCCC TCCTGCTGGA TCTGGAAGCT CTGGGTGTTT GTCAGGGCTA CCAAAACTTT 
CAAATGTGCT CGCCTTGGGA GCCTAAAATG TGTGTGCGAG CCGCACTATG CGATTTGCTC 
ATTCCTAAGG ATGATGAGCA TTGTGGGAAA TGCTCGCAGC CGGTGGAGGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001296818.1
CDS	337..2730
Translation	MTDELNELSPESEILKPEDAVPAYLNEMHPIGEDVDSDVEDDDMSCKVTVELLPDWLKPMEEEEVGVDELMSAGRINRRKTKEKPPKVKRGRGRPPKIRHGLSRSEFKKGLHADRTMKKKRVGSLMSRKRLSRKATSVPDPSLKAEVLPRTRQRVVRSSTSTPQSTKQVPDSPAGGTSAEFNSPPRLTRVSPPSIDTSRRSFVHSPSVKVGNVTQQIISTEPGKDVSELPLQLWLCPSLEPISPTQTQEILSQQTVGSPNFPEASLNGTMTVKESLTMTVKESLPLLSECEGCGCPFSAQKHGDTLCYRCRIKREKKRSPPNILCRKVRQDQWEVEKEKHPRKQMLQRPQRYGKTENDIVPDGGDDDDEEYWRLKKRNRRMCQRCDACRRDTDCGKCDFCMDKPKFGGSNKKRQKCRLRQCKFQSKLQFRNGMKGVPTSSLSPIKKMIKSNKLLKIKKRGRPRKKKFKNDPWEEEEEDYEVSDNDEDDQELRSSEMNGSQVRKHGKWSYTFKEDEDDAAYVEDVVDANIEEDSVILDNERSVMASNEMYNTTSGLSSQGLYYNVSGMPLSSHLIGSAPICNSGAVAMGEVIGSVPMADEMSQNGFLQIEMVKVGSSPSHYREEQQNTQQMHIADPQQEHTPVITQIFSLAGPETESDRDPGLIELFTSLGQSVLPAHWVCVIAKGPVLQLLQCSKLSTMADTVVQIEKGFFYQVTVQNQPLLLMHSLYSRHPTCLEKVEHVVSLLLDLEALGVCQGYQNFQMCSPWEPKMCVRAALCDLLIPKDDEHCGKCSQPVEG

Target ORF information:

RefSeq Version	NM_001309889.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio methyl-CpG binding domain protein 1a (mbd1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001309889.1

ORF Insert Sequence:

ATGACGGATG AGCTGAACGA GCTATCACCT GAATCTGAGA TCTTGAAACC AGAAGACGCT 
GTGCCAGCAT ACTTAAACGA GATGCATCCG ATCGGAGAAG ACGTTGATAG TGACGTTGAG 
GATGACGACA TGTCCTGCAA AGTGACGGTT GAGTTATTAC CTGATTGGTT GAAGCCTATG 
GAGGAGGAGG AAGTTGGTGT TGATGAGCTC ATGAGCGCTG GACGGATCAA CCGGAGAAAA 
ACTAAAGAAA AACCTCCGAA AGTAAAACGG GGCAGGGGTC GACCTCCTAA AATAAGACAT 
GGACTTAGTC GTTCAGAGTT CAAGAAAGGA CTGCATGCGG ATCGCACTAT GAAGAAAAAG 
AGGGTTGGAT CCCTAATGAG TCGCAAACGT TTATCCCGAA AAGCCACTTC CGTCCCAGAT 
CCATCGCTCA AGGCTGAAGT TTTACCAAGA ACCCGCCAAA GAGTTGTTCG CAGCTCAACA 
TCCACACCTC AAAGTACCAA ACAGGTCCCA GACTCTCCAG CGGGAGGCAC CAGCGCAGAA 
TTTAACAGCC CTCCGCGCCT CACAAGGGTC TCGCCGCCCA GTATCGACAC GTCCAGGCGC 
AGCTTCGTTC ACTCTCCTTC TGTTAAAGTG GGTAACGTCA CCCAGCAGAT AATCAGCACT 
GAACCTGGGA AAGATGTCTC TGAGCTGCCG CTTCAACTTT GGCTTTGTCC TTCTCTGGAG 
CCCATTAGCC CTACACAAAC TCAGGAAATA CTCTCGCAGC AAACCGTAGG GTCACCAAAT 
TTTCCAGAGG CTTCGCTGAA TGGGACAATG ACGGTGAAGG AGAGTCTGAC AATGACGGTG 
AAGGAGAGTC TTCCTTTGCT CAGTGAATGT GAAGGATGCG GATGTCCATT TTCAGCTCAG 
AAGCATGGAG ACACTTTGTG CTACAGATGC AGAATTAAAA GAGAGAAGAA GCGGTCTCCT 
CCAAACATTT TGTGTAGGAA GGTGAGGCAG GATCAGTGGG AGGTTGAGAA AGAAAAACAT 
CCAAGAAAGC AAATGCTGCA GAGACCACAA AGATATGGGA AAACCGAAAA TGATATTGTG 
CCTGATGGTG GTGATGATGA TGATGAAGAG TACTGGCGTT TAAAGAAGCG TAATCGGAGG 
ATGTGTCAGC GGTGTGATGC CTGCCGTCGT GATACAGACT GTGGGAAGTG CGACTTCTGC 
ATGGACAAGC CCAAGTTTGG TGGCAGTAAC AAGAAAAGGC AGAAATGTCG CTTGCGTCAG 
TGCAAGTTTC AGTCAAAGCT TCAATTTCGA AATGGAATGA AGGGAGTGCC GACTTCATCT 
CTTTCACCAA TAAAGAAAAT GATCAAATCC AATAAACTCC TCAAAATAAA GAAACGTGGG 
AGACCACGCA AAAAAAAATT CAAAAATGAC CCATGGGAGG AGGAGGAGGA GGATTATGAA 
GTGTCTGATA ATGATGAAGA TGATCAAGAG TTGAGGAGTT CTGAGATGAA CGGCTCCCAG 
GTCAGAAAAC ACGGGAAATG GAGTTACACT TTTAAGGAAG ATGAAGACGA CGCGGCCTAT 
GTTGAGGATG TGGTTGATGC CAACATTGAA GAGGATTCTG TTATTTTGGA TAATGAACGG 
TCAGTGATGG CGTCTAACGA GATGTACAAC ACCACCTCTG GACTCAGTTC TCAGGGATTA 
TACTACAATG TGTCAGGAAT GCCGCTGTCG TCTCATCTGA TTGGCTCAGC TCCTATCTGT 
AACAGCGGTG CAGTGGCTAT GGGTGAGGTG ATCGGCTCGG TGCCCATGGC GGACGAGATG 
AGCCAGAATG GATTTCTTCA GATCGAGATG GTGAAAGTGG GATCATCTCC ATCACATTAC 
AGAGAAGAAC AGCAGAACAC ACAACAAATG CACATTGCAG ACCCTCAACA GGAGCACACG 
CCGGTGATCA CTCAGATCTT CAGTCTGGCG GGTCCAGAGA CAGAAAGTGA TCGAGACCCA 
GGCTTGATCG AGCTGTTCAC GTCCCTCGGC CAGAGCGTCC TGCCGGCTCA CTGGGTGTGT 
GTGATCGCCA AAGGCCCTGT CCTGCAGCTC CTGCAGTGCT CTAAACTCTC CACCATGGCA 
GACACCGTGG TCCAGATCGA GAAAGGCTTC TTCTACCAGG TCACCGTCCA GAACCAGCCT 
CTGCTGCTCA TGCACAGCCT CTACTCTCGC CATCCCACCT GCCTGGAGAA AGTGGAGCAT 
GTAGTGTCCC TCCTGCTGGA TCTGGAAGCT CTGGGTGTTT GTCAGGGCTA CCAAAACTTT 
CAAATGTGCT CGCCTTGGGA GCCTAAAATG TGTGTGCGAG CCGCACTATG CGATTTGCTC 
ATTCCTAAGG ATGATGAGCA TTGTGGGAAA TGCTCGCAGC CGGTGGAGGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mbd1a ( XM_009303759.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302034.1 Danio rerio methyl-CpG binding domain protein 1a (mbd1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa47149
Clone ID Related Accession (Same CDS sequence)	XM_009303759.2 , XM_009303759.3
Accession Version	XM_009303759.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	methyl-CpG binding domain protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303759.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGATG AGCTGAACGA GCTATCACCT GAATCTGAGA TCTTGAAACC AGAAGACGCT 
GTGCCAGCAT ACTTAAACGA GATGCATCCG ATCGGAGAAG ACGTTGATAG TGACGTTGAG 
GATGACGACA TGTCCTGCAA AGTGACGGTT GAGTTATTAC CTGATTGGTT GAAGCCTATG 
GAGGAGGAGG AAGTTGGTGT TGATGAGCTC ATGAGCGCTG GACGGATCAA CCGGAGAAAA 
ACTAAAGAAA AACCTCCGAA AGTAAAACGG GGCAGGGGTC GACCTCCTAA AATAAGACAT 
GGACTTAGTC GTTCAGAGTT CAAGAAAGGA CTGCATGCGG ATCGCACTAT GAAGAAAAAG 
AGGGTTGGAT CCCTAATGAG TCGCAAACGT TTATCCCGAA AAGCCACTTC CGTCCCAGAT 
CCATCGCTCA AGGCTGAAGT TTTACCAAGA ACCCGCCAAA GAGTTGTTCG CAGCTCAACA 
TCCACACCTC AAAGTACCAA ACAGGTCCCA GACTCTCCAG CGGGAGGCAC CAGCGCAGAA 
TTTAACAGCC CTCCGCGCCT CACAAGGGTC TCGCCGCCCA GTATCGACAC GTCCAGGCGC 
AGCTTCGTTC ACTCTCCTTC TGTTAAAGTG GGTAACGTCA CCCAGCAGAT AATCAGCACT 
GAACCTGGGA AAGATGTCTC TGAGCTGCCG CTTCAACTTT GGCTTTGTCC TTCTCTGGAG 
CCCATTAGCC CTACACAAAC TCAGGAAATA CTCTCGCAGC AAACCGTAGG GTCACCAAAT 
TTTCCAGAGG CTTCGCTGAA TGGGACAATG ACGGTGAAGG AGAGTCTGAC AATGACGGTG 
AAGGAGAGTC TTCCTTTGCT CAGTGAATGT GAAGGATGCG GATGTCCATT TTCAGCTCAG 
AAGCATGGAG ACACTTTGTG CTACAGATGC AGAATTAAAA GAGAGAAGAA GCGGTCTCCT 
CCAAACATTT TGTGTAGGAA GGTGAGGCAG GATCAGTGGG AGGTTGAGAA AGAAAAACAT 
CCAAGAAAGC AAATGCTGAG ACCACAAAGA TATGGGAAAA CCGAAAATGA TATTGTGCCT 
GATGGTGGTG ATGATGATGA TGAAGAGTAC TGGCGTTTAA AGAAGCGTAA TCGGAGGATG 
TGTCAGCGGT GTGATGCCTG CCGTCGTGAT ACAGACTGTG GGAAGTGCGA CTTCTGCATG 
GACAAGCCCA AGTTTGGTGG CAGTAACAAG AAAAGGCAGA AATGTCGCTT GCGTCAGTGC 
AAGTTTCAGT CAAAGCTTCA ATTTCGAAAT GGAATGAAGG GAGTGCCGAC TTCATCTCTT 
TCACCAATAA AGAAAATGAT CAAATCCAAT AAACTCCTCA AAATAAAGAA ACGTGGGAGA 
CCACGCAAAA AAAAATTCAA AAATGACCCA TGGGAGGAGG AGGAGGAGGA TTATGAAGTG 
TCTGATAATG ATGAAGATGA TCAAGAGTTG AGGAGTTCTG AGATGAACGG CTCCCAGGTC 
AGAAAACACG GGAAATGGAG TTACACTTTT AAGGAAGATG AAGACGACGC GGCCTATGTT 
GAGGATGTGG TTGATGCCAA CATTGAAGAG GATTCTGTTA TTTTGGATAA TGAACGGTCA 
GTGATGGCGT CTAACGAGAT GTACAACACC ACCTCTGGAC TCAGTTCTCA GGGATTATAC 
TACAATGTGT CAGGAATGCC GCTGTCGTCT CATCTGATTG GCTCAGCTCC TATCTGTAAC 
AGCGGTGCAG TGGCTATGGG TGAGGTGATC GGCTCGGTGC CCATGGCGGA CGAGATGAGC 
CAGAATGGAT TTCTTCAGAT CGAGATGGTG AAAGTGGGAT CATCTCCATC ACATTACAGA 
GAAGAACAGC AGAACACACA ACAAATGCAC ATTGCAGACC CTCAACAGGA GCACACGCCG 
GTGATCACTC AGATCTTCAG TCTGGCGGGT CCAGAGACAG AAAGTGATCG AGACCCAGGC 
TTGATCGAGC TGTTCACGTC CCTCGGCCAG AGCGTCCTGC CGGCTCACTG GGTGTGTGTG 
ATCGCCAAAG GCCCTGTCCT GCAGCTCCTG CAGTGCTCTA AACTCTCCAC CATGGCAGAC 
ACCGTGGTCC AGATCGAGAA AGGCTTCTTC TACCAGGTCA CCGTCCAGAA CCAGCCTCTG 
CTGCTCATGC ACAGCCTCTA CTCTCGCCAT CCCACCTGCC TGGAGAAAGT GGAGCATGTA 
GTGTCCCTCC TGCTGGATCT GGAAGCTCTG GGTGTTTGTC AGGGCTACCA AAACTTTCAA 
ATGTGCTCGC CTTGGGAGCC TAAAATGTGT GTGCGAGCCG CACTATGCGA TTTGCTCATT 
CCTAAGGATG ATGAGCATTG TGGGAAATGC TCGCAGCCGG TGGAGGGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302034.1
CDS	338..2728
Translation	MTDELNELSPESEILKPEDAVPAYLNEMHPIGEDVDSDVEDDDMSCKVTVELLPDWLKPMEEEEVGVDELMSAGRINRRKTKEKPPKVKRGRGRPPKIRHGLSRSEFKKGLHADRTMKKKRVGSLMSRKRLSRKATSVPDPSLKAEVLPRTRQRVVRSSTSTPQSTKQVPDSPAGGTSAEFNSPPRLTRVSPPSIDTSRRSFVHSPSVKVGNVTQQIISTEPGKDVSELPLQLWLCPSLEPISPTQTQEILSQQTVGSPNFPEASLNGTMTVKESLTMTVKESLPLLSECEGCGCPFSAQKHGDTLCYRCRIKREKKRSPPNILCRKVRQDQWEVEKEKHPRKQMLRPQRYGKTENDIVPDGGDDDDEEYWRLKKRNRRMCQRCDACRRDTDCGKCDFCMDKPKFGGSNKKRQKCRLRQCKFQSKLQFRNGMKGVPTSSLSPIKKMIKSNKLLKIKKRGRPRKKKFKNDPWEEEEEDYEVSDNDEDDQELRSSEMNGSQVRKHGKWSYTFKEDEDDAAYVEDVVDANIEEDSVILDNERSVMASNEMYNTTSGLSSQGLYYNVSGMPLSSHLIGSAPICNSGAVAMGEVIGSVPMADEMSQNGFLQIEMVKVGSSPSHYREEQQNTQQMHIADPQQEHTPVITQIFSLAGPETESDRDPGLIELFTSLGQSVLPAHWVCVIAKGPVLQLLQCSKLSTMADTVVQIEKGFFYQVTVQNQPLLLMHSLYSRHPTCLEKVEHVVSLLLDLEALGVCQGYQNFQMCSPWEPKMCVRAALCDLLIPKDDEHCGKCSQPVEG

Target ORF information:

RefSeq Version	XM_009303759.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio methyl-CpG binding domain protein 1a (mbd1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303759.2

ORF Insert Sequence:

ATGACGGATG AGCTGAACGA GCTATCACCT GAATCTGAGA TCTTGAAACC AGAAGACGCT 
GTGCCAGCAT ACTTAAACGA GATGCATCCG ATCGGAGAAG ACGTTGATAG TGACGTTGAG 
GATGACGACA TGTCCTGCAA AGTGACGGTT GAGTTATTAC CTGATTGGTT GAAGCCTATG 
GAGGAGGAGG AAGTTGGTGT TGATGAGCTC ATGAGCGCTG GACGGATCAA CCGGAGAAAA 
ACTAAAGAAA AACCTCCGAA AGTAAAACGG GGCAGGGGTC GACCTCCTAA AATAAGACAT 
GGACTTAGTC GTTCAGAGTT CAAGAAAGGA CTGCATGCGG ATCGCACTAT GAAGAAAAAG 
AGGGTTGGAT CCCTAATGAG TCGCAAACGT TTATCCCGAA AAGCCACTTC CGTCCCAGAT 
CCATCGCTCA AGGCTGAAGT TTTACCAAGA ACCCGCCAAA GAGTTGTTCG CAGCTCAACA 
TCCACACCTC AAAGTACCAA ACAGGTCCCA GACTCTCCAG CGGGAGGCAC CAGCGCAGAA 
TTTAACAGCC CTCCGCGCCT CACAAGGGTC TCGCCGCCCA GTATCGACAC GTCCAGGCGC 
AGCTTCGTTC ACTCTCCTTC TGTTAAAGTG GGTAACGTCA CCCAGCAGAT AATCAGCACT 
GAACCTGGGA AAGATGTCTC TGAGCTGCCG CTTCAACTTT GGCTTTGTCC TTCTCTGGAG 
CCCATTAGCC CTACACAAAC TCAGGAAATA CTCTCGCAGC AAACCGTAGG GTCACCAAAT 
TTTCCAGAGG CTTCGCTGAA TGGGACAATG ACGGTGAAGG AGAGTCTGAC AATGACGGTG 
AAGGAGAGTC TTCCTTTGCT CAGTGAATGT GAAGGATGCG GATGTCCATT TTCAGCTCAG 
AAGCATGGAG ACACTTTGTG CTACAGATGC AGAATTAAAA GAGAGAAGAA GCGGTCTCCT 
CCAAACATTT TGTGTAGGAA GGTGAGGCAG GATCAGTGGG AGGTTGAGAA AGAAAAACAT 
CCAAGAAAGC AAATGCTGAG ACCACAAAGA TATGGGAAAA CCGAAAATGA TATTGTGCCT 
GATGGTGGTG ATGATGATGA TGAAGAGTAC TGGCGTTTAA AGAAGCGTAA TCGGAGGATG 
TGTCAGCGGT GTGATGCCTG CCGTCGTGAT ACAGACTGTG GGAAGTGCGA CTTCTGCATG 
GACAAGCCCA AGTTTGGTGG CAGTAACAAG AAAAGGCAGA AATGTCGCTT GCGTCAGTGC 
AAGTTTCAGT CAAAGCTTCA ATTTCGAAAT GGAATGAAGG GAGTGCCGAC TTCATCTCTT 
TCACCAATAA AGAAAATGAT CAAATCCAAT AAACTCCTCA AAATAAAGAA ACGTGGGAGA 
CCACGCAAAA AAAAATTCAA AAATGACCCA TGGGAGGAGG AGGAGGAGGA TTATGAAGTG 
TCTGATAATG ATGAAGATGA TCAAGAGTTG AGGAGTTCTG AGATGAACGG CTCCCAGGTC 
AGAAAACACG GGAAATGGAG TTACACTTTT AAGGAAGATG AAGACGACGC GGCCTATGTT 
GAGGATGTGG TTGATGCCAA CATTGAAGAG GATTCTGTTA TTTTGGATAA TGAACGGTCA 
GTGATGGCGT CTAACGAGAT GTACAACACC ACCTCTGGAC TCAGTTCTCA GGGATTATAC 
TACAATGTGT CAGGAATGCC GCTGTCGTCT CATCTGATTG GCTCAGCTCC TATCTGTAAC 
AGCGGTGCAG TGGCTATGGG TGAGGTGATC GGCTCGGTGC CCATGGCGGA CGAGATGAGC 
CAGAATGGAT TTCTTCAGAT CGAGATGGTG AAAGTGGGAT CATCTCCATC ACATTACAGA 
GAAGAACAGC AGAACACACA ACAAATGCAC ATTGCAGACC CTCAACAGGA GCACACGCCG 
GTGATCACTC AGATCTTCAG TCTGGCGGGT CCAGAGACAG AAAGTGATCG AGACCCAGGC 
TTGATCGAGC TGTTCACGTC CCTCGGCCAG AGCGTCCTGC CGGCTCACTG GGTGTGTGTG 
ATCGCCAAAG GCCCTGTCCT GCAGCTCCTG CAGTGCTCTA AACTCTCCAC CATGGCAGAC 
ACCGTGGTCC AGATCGAGAA AGGCTTCTTC TACCAGGTCA CCGTCCAGAA CCAGCCTCTG 
CTGCTCATGC ACAGCCTCTA CTCTCGCCAT CCCACCTGCC TGGAGAAAGT GGAGCATGTA 
GTGTCCCTCC TGCTGGATCT GGAAGCTCTG GGTGTTTGTC AGGGCTACCA AAACTTTCAA 
ATGTGCTCGC CTTGGGAGCC TAAAATGTGT GTGCGAGCCG CACTATGCGA TTTGCTCATT 
CCTAAGGATG ATGAGCATTG TGGGAAATGC TCGCAGCCGG TGGAGGGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mbd1a ( XM_009303759.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302034.1 Danio rerio methyl-CpG binding domain protein 1a (mbd1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa47149
Clone ID Related Accession (Same CDS sequence)	XM_009303759.2 , XM_009303759.3
Accession Version	XM_009303759.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	methyl-CpG binding domain protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303759.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGATG AGCTGAACGA GCTATCACCT GAATCTGAGA TCTTGAAACC AGAAGACGCT 
GTGCCAGCAT ACTTAAACGA GATGCATCCG ATCGGAGAAG ACGTTGATAG TGACGTTGAG 
GATGACGACA TGTCCTGCAA AGTGACGGTT GAGTTATTAC CTGATTGGTT GAAGCCTATG 
GAGGAGGAGG AAGTTGGTGT TGATGAGCTC ATGAGCGCTG GACGGATCAA CCGGAGAAAA 
ACTAAAGAAA AACCTCCGAA AGTAAAACGG GGCAGGGGTC GACCTCCTAA AATAAGACAT 
GGACTTAGTC GTTCAGAGTT CAAGAAAGGA CTGCATGCGG ATCGCACTAT GAAGAAAAAG 
AGGGTTGGAT CCCTAATGAG TCGCAAACGT TTATCCCGAA AAGCCACTTC CGTCCCAGAT 
CCATCGCTCA AGGCTGAAGT TTTACCAAGA ACCCGCCAAA GAGTTGTTCG CAGCTCAACA 
TCCACACCTC AAAGTACCAA ACAGGTCCCA GACTCTCCAG CGGGAGGCAC CAGCGCAGAA 
TTTAACAGCC CTCCGCGCCT CACAAGGGTC TCGCCGCCCA GTATCGACAC GTCCAGGCGC 
AGCTTCGTTC ACTCTCCTTC TGTTAAAGTG GGTAACGTCA CCCAGCAGAT AATCAGCACT 
GAACCTGGGA AAGATGTCTC TGAGCTGCCG CTTCAACTTT GGCTTTGTCC TTCTCTGGAG 
CCCATTAGCC CTACACAAAC TCAGGAAATA CTCTCGCAGC AAACCGTAGG GTCACCAAAT 
TTTCCAGAGG CTTCGCTGAA TGGGACAATG ACGGTGAAGG AGAGTCTGAC AATGACGGTG 
AAGGAGAGTC TTCCTTTGCT CAGTGAATGT GAAGGATGCG GATGTCCATT TTCAGCTCAG 
AAGCATGGAG ACACTTTGTG CTACAGATGC AGAATTAAAA GAGAGAAGAA GCGGTCTCCT 
CCAAACATTT TGTGTAGGAA GGTGAGGCAG GATCAGTGGG AGGTTGAGAA AGAAAAACAT 
CCAAGAAAGC AAATGCTGAG ACCACAAAGA TATGGGAAAA CCGAAAATGA TATTGTGCCT 
GATGGTGGTG ATGATGATGA TGAAGAGTAC TGGCGTTTAA AGAAGCGTAA TCGGAGGATG 
TGTCAGCGGT GTGATGCCTG CCGTCGTGAT ACAGACTGTG GGAAGTGCGA CTTCTGCATG 
GACAAGCCCA AGTTTGGTGG CAGTAACAAG AAAAGGCAGA AATGTCGCTT GCGTCAGTGC 
AAGTTTCAGT CAAAGCTTCA ATTTCGAAAT GGAATGAAGG GAGTGCCGAC TTCATCTCTT 
TCACCAATAA AGAAAATGAT CAAATCCAAT AAACTCCTCA AAATAAAGAA ACGTGGGAGA 
CCACGCAAAA AAAAATTCAA AAATGACCCA TGGGAGGAGG AGGAGGAGGA TTATGAAGTG 
TCTGATAATG ATGAAGATGA TCAAGAGTTG AGGAGTTCTG AGATGAACGG CTCCCAGGTC 
AGAAAACACG GGAAATGGAG TTACACTTTT AAGGAAGATG AAGACGACGC GGCCTATGTT 
GAGGATGTGG TTGATGCCAA CATTGAAGAG GATTCTGTTA TTTTGGATAA TGAACGGTCA 
GTGATGGCGT CTAACGAGAT GTACAACACC ACCTCTGGAC TCAGTTCTCA GGGATTATAC 
TACAATGTGT CAGGAATGCC GCTGTCGTCT CATCTGATTG GCTCAGCTCC TATCTGTAAC 
AGCGGTGCAG TGGCTATGGG TGAGGTGATC GGCTCGGTGC CCATGGCGGA CGAGATGAGC 
CAGAATGGAT TTCTTCAGAT CGAGATGGTG AAAGTGGGAT CATCTCCATC ACATTACAGA 
GAAGAACAGC AGAACACACA ACAAATGCAC ATTGCAGACC CTCAACAGGA GCACACGCCG 
GTGATCACTC AGATCTTCAG TCTGGCGGGT CCAGAGACAG AAAGTGATCG AGACCCAGGC 
TTGATCGAGC TGTTCACGTC CCTCGGCCAG AGCGTCCTGC CGGCTCACTG GGTGTGTGTG 
ATCGCCAAAG GCCCTGTCCT GCAGCTCCTG CAGTGCTCTA AACTCTCCAC CATGGCAGAC 
ACCGTGGTCC AGATCGAGAA AGGCTTCTTC TACCAGGTCA CCGTCCAGAA CCAGCCTCTG 
CTGCTCATGC ACAGCCTCTA CTCTCGCCAT CCCACCTGCC TGGAGAAAGT GGAGCATGTA 
GTGTCCCTCC TGCTGGATCT GGAAGCTCTG GGTGTTTGTC AGGGCTACCA AAACTTTCAA 
ATGTGCTCGC CTTGGGAGCC TAAAATGTGT GTGCGAGCCG CACTATGCGA TTTGCTCATT 
CCTAAGGATG ATGAGCATTG TGGGAAATGC TCGCAGCCGG TGGAGGGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302034.1
CDS	380..2770
Translation	MTDELNELSPESEILKPEDAVPAYLNEMHPIGEDVDSDVEDDDMSCKVTVELLPDWLKPMEEEEVGVDELMSAGRINRRKTKEKPPKVKRGRGRPPKIRHGLSRSEFKKGLHADRTMKKKRVGSLMSRKRLSRKATSVPDPSLKAEVLPRTRQRVVRSSTSTPQSTKQVPDSPAGGTSAEFNSPPRLTRVSPPSIDTSRRSFVHSPSVKVGNVTQQIISTEPGKDVSELPLQLWLCPSLEPISPTQTQEILSQQTVGSPNFPEASLNGTMTVKESLTMTVKESLPLLSECEGCGCPFSAQKHGDTLCYRCRIKREKKRSPPNILCRKVRQDQWEVEKEKHPRKQMLRPQRYGKTENDIVPDGGDDDDEEYWRLKKRNRRMCQRCDACRRDTDCGKCDFCMDKPKFGGSNKKRQKCRLRQCKFQSKLQFRNGMKGVPTSSLSPIKKMIKSNKLLKIKKRGRPRKKKFKNDPWEEEEEDYEVSDNDEDDQELRSSEMNGSQVRKHGKWSYTFKEDEDDAAYVEDVVDANIEEDSVILDNERSVMASNEMYNTTSGLSSQGLYYNVSGMPLSSHLIGSAPICNSGAVAMGEVIGSVPMADEMSQNGFLQIEMVKVGSSPSHYREEQQNTQQMHIADPQQEHTPVITQIFSLAGPETESDRDPGLIELFTSLGQSVLPAHWVCVIAKGPVLQLLQCSKLSTMADTVVQIEKGFFYQVTVQNQPLLLMHSLYSRHPTCLEKVEHVVSLLLDLEALGVCQGYQNFQMCSPWEPKMCVRAALCDLLIPKDDEHCGKCSQPVEG

Target ORF information:

RefSeq Version	XM_009303759.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio methyl-CpG binding domain protein 1a (mbd1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303759.3

ORF Insert Sequence:

ATGACGGATG AGCTGAACGA GCTATCACCT GAATCTGAGA TCTTGAAACC AGAAGACGCT 
GTGCCAGCAT ACTTAAACGA GATGCATCCG ATCGGAGAAG ACGTTGATAG TGACGTTGAG 
GATGACGACA TGTCCTGCAA AGTGACGGTT GAGTTATTAC CTGATTGGTT GAAGCCTATG 
GAGGAGGAGG AAGTTGGTGT TGATGAGCTC ATGAGCGCTG GACGGATCAA CCGGAGAAAA 
ACTAAAGAAA AACCTCCGAA AGTAAAACGG GGCAGGGGTC GACCTCCTAA AATAAGACAT 
GGACTTAGTC GTTCAGAGTT CAAGAAAGGA CTGCATGCGG ATCGCACTAT GAAGAAAAAG 
AGGGTTGGAT CCCTAATGAG TCGCAAACGT TTATCCCGAA AAGCCACTTC CGTCCCAGAT 
CCATCGCTCA AGGCTGAAGT TTTACCAAGA ACCCGCCAAA GAGTTGTTCG CAGCTCAACA 
TCCACACCTC AAAGTACCAA ACAGGTCCCA GACTCTCCAG CGGGAGGCAC CAGCGCAGAA 
TTTAACAGCC CTCCGCGCCT CACAAGGGTC TCGCCGCCCA GTATCGACAC GTCCAGGCGC 
AGCTTCGTTC ACTCTCCTTC TGTTAAAGTG GGTAACGTCA CCCAGCAGAT AATCAGCACT 
GAACCTGGGA AAGATGTCTC TGAGCTGCCG CTTCAACTTT GGCTTTGTCC TTCTCTGGAG 
CCCATTAGCC CTACACAAAC TCAGGAAATA CTCTCGCAGC AAACCGTAGG GTCACCAAAT 
TTTCCAGAGG CTTCGCTGAA TGGGACAATG ACGGTGAAGG AGAGTCTGAC AATGACGGTG 
AAGGAGAGTC TTCCTTTGCT CAGTGAATGT GAAGGATGCG GATGTCCATT TTCAGCTCAG 
AAGCATGGAG ACACTTTGTG CTACAGATGC AGAATTAAAA GAGAGAAGAA GCGGTCTCCT 
CCAAACATTT TGTGTAGGAA GGTGAGGCAG GATCAGTGGG AGGTTGAGAA AGAAAAACAT 
CCAAGAAAGC AAATGCTGAG ACCACAAAGA TATGGGAAAA CCGAAAATGA TATTGTGCCT 
GATGGTGGTG ATGATGATGA TGAAGAGTAC TGGCGTTTAA AGAAGCGTAA TCGGAGGATG 
TGTCAGCGGT GTGATGCCTG CCGTCGTGAT ACAGACTGTG GGAAGTGCGA CTTCTGCATG 
GACAAGCCCA AGTTTGGTGG CAGTAACAAG AAAAGGCAGA AATGTCGCTT GCGTCAGTGC 
AAGTTTCAGT CAAAGCTTCA ATTTCGAAAT GGAATGAAGG GAGTGCCGAC TTCATCTCTT 
TCACCAATAA AGAAAATGAT CAAATCCAAT AAACTCCTCA AAATAAAGAA ACGTGGGAGA 
CCACGCAAAA AAAAATTCAA AAATGACCCA TGGGAGGAGG AGGAGGAGGA TTATGAAGTG 
TCTGATAATG ATGAAGATGA TCAAGAGTTG AGGAGTTCTG AGATGAACGG CTCCCAGGTC 
AGAAAACACG GGAAATGGAG TTACACTTTT AAGGAAGATG AAGACGACGC GGCCTATGTT 
GAGGATGTGG TTGATGCCAA CATTGAAGAG GATTCTGTTA TTTTGGATAA TGAACGGTCA 
GTGATGGCGT CTAACGAGAT GTACAACACC ACCTCTGGAC TCAGTTCTCA GGGATTATAC 
TACAATGTGT CAGGAATGCC GCTGTCGTCT CATCTGATTG GCTCAGCTCC TATCTGTAAC 
AGCGGTGCAG TGGCTATGGG TGAGGTGATC GGCTCGGTGC CCATGGCGGA CGAGATGAGC 
CAGAATGGAT TTCTTCAGAT CGAGATGGTG AAAGTGGGAT CATCTCCATC ACATTACAGA 
GAAGAACAGC AGAACACACA ACAAATGCAC ATTGCAGACC CTCAACAGGA GCACACGCCG 
GTGATCACTC AGATCTTCAG TCTGGCGGGT CCAGAGACAG AAAGTGATCG AGACCCAGGC 
TTGATCGAGC TGTTCACGTC CCTCGGCCAG AGCGTCCTGC CGGCTCACTG GGTGTGTGTG 
ATCGCCAAAG GCCCTGTCCT GCAGCTCCTG CAGTGCTCTA AACTCTCCAC CATGGCAGAC 
ACCGTGGTCC AGATCGAGAA AGGCTTCTTC TACCAGGTCA CCGTCCAGAA CCAGCCTCTG 
CTGCTCATGC ACAGCCTCTA CTCTCGCCAT CCCACCTGCC TGGAGAAAGT GGAGCATGTA 
GTGTCCCTCC TGCTGGATCT GGAAGCTCTG GGTGTTTGTC AGGGCTACCA AAACTTTCAA 
ATGTGCTCGC CTTGGGAGCC TAAAATGTGT GTGCGAGCCG CACTATGCGA TTTGCTCATT 
CCTAAGGATG ATGAGCATTG TGGGAAATGC TCGCAGCCGG TGGAGGGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mbd1a ( XM_009303760.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302035.1 Danio rerio methyl-CpG binding domain protein 1a (mbd1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa47150
Clone ID Related Accession (Same CDS sequence)	XM_009303760.3 , XM_009303760.2
Accession Version	XM_009303760.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	methyl-CpG binding domain protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303760.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGATG AGCTGAACGA GCTATCACCT GAATCTGAGA TCTTGAAACC AGAAGACGCT 
GTGCCAGCAT ACTTAAACGA GATGCATCCG ATCGGAGAAG ACGTTGATAG TGACGTTGAG 
GATGACGACA TGTCCTGCAA AGTGACGGTT GAGTTATTAC CTGATTGGTT GAAGCCTATG 
GAGGAGGAGG AAGTTGGTGT TGATGAGCTC ATGAGCGCTG GACGGATCAA CCGGAGAAAA 
ACTAAAGAAA AACCTCCGAA AGTAAAACGG GGCAGGGGTC GACCTCCTAA AATAAGACAT 
GGACTTAGTC GTTCAGAGTT CAAGAAAGGA CTGCATGCGG ATCGCACTAT GAAGAAAAAG 
AGGGTTGGAT CCCTAATGAG TCGCAAACGT TTATCCCGAA AAGCCACTTC CGTCCCAGAT 
CCATCGCTCA AGGCTGAAGT TTTACCAAGA ACCCGCCAAA GAGTTGTTCG CAGCTCAACA 
TCCACACCTC AAAGTACCAA ACAGGTCCCA GACTCTCCAG CGGGAGGCAC CAGCGCAGAA 
TTTAACAGCC CTCCGCGCCT CACAAGGGTC TCGCCGCCCA GTATCGACAC GTCCAGGCGC 
AGCTTCGTTC ACTCTCCTTC TGTTAAAGTG GGTAACGTCA CCCAGCAGAT AATCAGCACT 
GAACCTGGGA AAGATGTCTC TGAGCTGCCG CTTCAACTTT GGCTTTGTCC TTCTCTGGAG 
CCCATTAGCC CTACACAAAC TCAGGAAATA CTCTCGCAGC AAACCGTAGG GTCACCAAAT 
TTTCCAGAGG CTTCGCTGAA TGGGACAATG ACGGTGAAGG AGAGTCTGAC AATGACGGTG 
AAGGAGAGTC TTCCTTTGCT CAGTGAATGT GAAGGATGCG GATGTCCATT TTCAGCTCAG 
AAGCATGGAG ACACTTTGTG CTACAGATGC AGAATTAAAA GAGAGAAGAA GCGGTCTCCT 
CCAAACATTT TGTGTAGGAA GCAGAGACCA CAAAGATATG GGAAAACCGA AAATGATATT 
GTGCCTGATG GTGGTGATGA TGATGATGAA GAGTACTGGC GTTTAAAGAA GCGTAATCGG 
AGGATGTGTC AGCGGTGTGA TGCCTGCCGT CGTGATACAG ACTGTGGGAA GTGCGACTTC 
TGCATGGACA AGCCCAAGTT TGGTGGCAGT AACAAGAAAA GGCAGAAATG TCGCTTGCGT 
CAGTGCAAGT TTCAGTCAAA GCTTCAATTT CGAAATGGAA TGAAGGGAGT GCCGACTTCA 
TCTCTTTCAC CAATAAAGAA AATGATCAAA TCCAATAAAC TCCTCAAAAT AAAGAAACGT 
GGGAGACCAC GCAAAAAAAA ATTCAAAAAT GACCCATGGG AGGAGGAGGA GGAGGATTAT 
GAAGTGTCTG ATAATGATGA AGATGATCAA GAGTTGAGGA GTTCTGAGAT GAACGGCTCC 
CAGGTCAGAA AACACGGGAA ATGGAGTTAC ACTTTTAAGG AAGATGAAGA CGACGCGGCC 
TATGTTGAGG ATGTGGTTGA TGCCAACATT GAAGAGGATT CTGTTATTTT GGATAATGAA 
CGGTCAGTGA TGGCGTCTAA CGAGATGTAC AACACCACCT CTGGACTCAG TTCTCAGGGA 
TTATACTACA ATGTGTCAGG AATGCCGCTG TCGTCTCATC TGATTGGCTC AGCTCCTATC 
TGTAACAGCG GTGCAGTGGC TATGGGTGAG GTGATCGGCT CGGTGCCCAT GGCGGACGAG 
ATGAGCCAGA ATGGATTTCT TCAGATCGAG ATGGTGAAAG TGGGATCATC TCCATCACAT 
TACAGAGAAG AACAGCAGAA CACACAACAA ATGCACATTG CAGACCCTCA ACAGGAGCAC 
ACGCCGGTGA TCACTCAGAT CTTCAGTCTG GCGGGTCCAG AGACAGAAAG TGATCGAGAC 
CCAGGCTTGA TCGAGCTGTT CACGTCCCTC GGCCAGAGCG TCCTGCCGGC TCACTGGGTG 
TGTGTGATCG CCAAAGGCCC TGTCCTGCAG CTCCTGCAGT GCTCTAAACT CTCCACCATG 
GCAGACACCG TGGTCCAGAT CGAGAAAGGC TTCTTCTACC AGGTCACCGT CCAGAACCAG 
CCTCTGCTGC TCATGCACAG CCTCTACTCT CGCCATCCCA CCTGCCTGGA GAAAGTGGAG 
CATGTAGTGT CCCTCCTGCT GGATCTGGAA GCTCTGGGTG TTTGTCAGGG CTACCAAAAC 
TTTCAAATGT GCTCGCCTTG GGAGCCTAAA ATGTGTGTGC GAGCCGCACT ATGCGATTTG 
CTCATTCCTA AGGATGATGA GCATTGTGGG AAATGCTCGC AGCCGGTGGA GGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302035.1
CDS	373..2709
Translation	MTDELNELSPESEILKPEDAVPAYLNEMHPIGEDVDSDVEDDDMSCKVTVELLPDWLKPMEEEEVGVDELMSAGRINRRKTKEKPPKVKRGRGRPPKIRHGLSRSEFKKGLHADRTMKKKRVGSLMSRKRLSRKATSVPDPSLKAEVLPRTRQRVVRSSTSTPQSTKQVPDSPAGGTSAEFNSPPRLTRVSPPSIDTSRRSFVHSPSVKVGNVTQQIISTEPGKDVSELPLQLWLCPSLEPISPTQTQEILSQQTVGSPNFPEASLNGTMTVKESLTMTVKESLPLLSECEGCGCPFSAQKHGDTLCYRCRIKREKKRSPPNILCRKQRPQRYGKTENDIVPDGGDDDDEEYWRLKKRNRRMCQRCDACRRDTDCGKCDFCMDKPKFGGSNKKRQKCRLRQCKFQSKLQFRNGMKGVPTSSLSPIKKMIKSNKLLKIKKRGRPRKKKFKNDPWEEEEEDYEVSDNDEDDQELRSSEMNGSQVRKHGKWSYTFKEDEDDAAYVEDVVDANIEEDSVILDNERSVMASNEMYNTTSGLSSQGLYYNVSGMPLSSHLIGSAPICNSGAVAMGEVIGSVPMADEMSQNGFLQIEMVKVGSSPSHYREEQQNTQQMHIADPQQEHTPVITQIFSLAGPETESDRDPGLIELFTSLGQSVLPAHWVCVIAKGPVLQLLQCSKLSTMADTVVQIEKGFFYQVTVQNQPLLLMHSLYSRHPTCLEKVEHVVSLLLDLEALGVCQGYQNFQMCSPWEPKMCVRAALCDLLIPKDDEHCGKCSQPVEG

Target ORF information:

RefSeq Version	XM_009303760.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio methyl-CpG binding domain protein 1a (mbd1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303760.3

ORF Insert Sequence:

ATGACGGATG AGCTGAACGA GCTATCACCT GAATCTGAGA TCTTGAAACC AGAAGACGCT 
GTGCCAGCAT ACTTAAACGA GATGCATCCG ATCGGAGAAG ACGTTGATAG TGACGTTGAG 
GATGACGACA TGTCCTGCAA AGTGACGGTT GAGTTATTAC CTGATTGGTT GAAGCCTATG 
GAGGAGGAGG AAGTTGGTGT TGATGAGCTC ATGAGCGCTG GACGGATCAA CCGGAGAAAA 
ACTAAAGAAA AACCTCCGAA AGTAAAACGG GGCAGGGGTC GACCTCCTAA AATAAGACAT 
GGACTTAGTC GTTCAGAGTT CAAGAAAGGA CTGCATGCGG ATCGCACTAT GAAGAAAAAG 
AGGGTTGGAT CCCTAATGAG TCGCAAACGT TTATCCCGAA AAGCCACTTC CGTCCCAGAT 
CCATCGCTCA AGGCTGAAGT TTTACCAAGA ACCCGCCAAA GAGTTGTTCG CAGCTCAACA 
TCCACACCTC AAAGTACCAA ACAGGTCCCA GACTCTCCAG CGGGAGGCAC CAGCGCAGAA 
TTTAACAGCC CTCCGCGCCT CACAAGGGTC TCGCCGCCCA GTATCGACAC GTCCAGGCGC 
AGCTTCGTTC ACTCTCCTTC TGTTAAAGTG GGTAACGTCA CCCAGCAGAT AATCAGCACT 
GAACCTGGGA AAGATGTCTC TGAGCTGCCG CTTCAACTTT GGCTTTGTCC TTCTCTGGAG 
CCCATTAGCC CTACACAAAC TCAGGAAATA CTCTCGCAGC AAACCGTAGG GTCACCAAAT 
TTTCCAGAGG CTTCGCTGAA TGGGACAATG ACGGTGAAGG AGAGTCTGAC AATGACGGTG 
AAGGAGAGTC TTCCTTTGCT CAGTGAATGT GAAGGATGCG GATGTCCATT TTCAGCTCAG 
AAGCATGGAG ACACTTTGTG CTACAGATGC AGAATTAAAA GAGAGAAGAA GCGGTCTCCT 
CCAAACATTT TGTGTAGGAA GCAGAGACCA CAAAGATATG GGAAAACCGA AAATGATATT 
GTGCCTGATG GTGGTGATGA TGATGATGAA GAGTACTGGC GTTTAAAGAA GCGTAATCGG 
AGGATGTGTC AGCGGTGTGA TGCCTGCCGT CGTGATACAG ACTGTGGGAA GTGCGACTTC 
TGCATGGACA AGCCCAAGTT TGGTGGCAGT AACAAGAAAA GGCAGAAATG TCGCTTGCGT 
CAGTGCAAGT TTCAGTCAAA GCTTCAATTT CGAAATGGAA TGAAGGGAGT GCCGACTTCA 
TCTCTTTCAC CAATAAAGAA AATGATCAAA TCCAATAAAC TCCTCAAAAT AAAGAAACGT 
GGGAGACCAC GCAAAAAAAA ATTCAAAAAT GACCCATGGG AGGAGGAGGA GGAGGATTAT 
GAAGTGTCTG ATAATGATGA AGATGATCAA GAGTTGAGGA GTTCTGAGAT GAACGGCTCC 
CAGGTCAGAA AACACGGGAA ATGGAGTTAC ACTTTTAAGG AAGATGAAGA CGACGCGGCC 
TATGTTGAGG ATGTGGTTGA TGCCAACATT GAAGAGGATT CTGTTATTTT GGATAATGAA 
CGGTCAGTGA TGGCGTCTAA CGAGATGTAC AACACCACCT CTGGACTCAG TTCTCAGGGA 
TTATACTACA ATGTGTCAGG AATGCCGCTG TCGTCTCATC TGATTGGCTC AGCTCCTATC 
TGTAACAGCG GTGCAGTGGC TATGGGTGAG GTGATCGGCT CGGTGCCCAT GGCGGACGAG 
ATGAGCCAGA ATGGATTTCT TCAGATCGAG ATGGTGAAAG TGGGATCATC TCCATCACAT 
TACAGAGAAG AACAGCAGAA CACACAACAA ATGCACATTG CAGACCCTCA ACAGGAGCAC 
ACGCCGGTGA TCACTCAGAT CTTCAGTCTG GCGGGTCCAG AGACAGAAAG TGATCGAGAC 
CCAGGCTTGA TCGAGCTGTT CACGTCCCTC GGCCAGAGCG TCCTGCCGGC TCACTGGGTG 
TGTGTGATCG CCAAAGGCCC TGTCCTGCAG CTCCTGCAGT GCTCTAAACT CTCCACCATG 
GCAGACACCG TGGTCCAGAT CGAGAAAGGC TTCTTCTACC AGGTCACCGT CCAGAACCAG 
CCTCTGCTGC TCATGCACAG CCTCTACTCT CGCCATCCCA CCTGCCTGGA GAAAGTGGAG 
CATGTAGTGT CCCTCCTGCT GGATCTGGAA GCTCTGGGTG TTTGTCAGGG CTACCAAAAC 
TTTCAAATGT GCTCGCCTTG GGAGCCTAAA ATGTGTGTGC GAGCCGCACT ATGCGATTTG 
CTCATTCCTA AGGATGATGA GCATTGTGGG AAATGCTCGC AGCCGGTGGA GGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mbd1a ( XM_009303760.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302035.1 Danio rerio methyl-CpG binding domain protein 1a (mbd1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa47150
Clone ID Related Accession (Same CDS sequence)	XM_009303760.3 , XM_009303760.2
Accession Version	XM_009303760.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	methyl-CpG binding domain protein isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303760.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGATG AGCTGAACGA GCTATCACCT GAATCTGAGA TCTTGAAACC AGAAGACGCT 
GTGCCAGCAT ACTTAAACGA GATGCATCCG ATCGGAGAAG ACGTTGATAG TGACGTTGAG 
GATGACGACA TGTCCTGCAA AGTGACGGTT GAGTTATTAC CTGATTGGTT GAAGCCTATG 
GAGGAGGAGG AAGTTGGTGT TGATGAGCTC ATGAGCGCTG GACGGATCAA CCGGAGAAAA 
ACTAAAGAAA AACCTCCGAA AGTAAAACGG GGCAGGGGTC GACCTCCTAA AATAAGACAT 
GGACTTAGTC GTTCAGAGTT CAAGAAAGGA CTGCATGCGG ATCGCACTAT GAAGAAAAAG 
AGGGTTGGAT CCCTAATGAG TCGCAAACGT TTATCCCGAA AAGCCACTTC CGTCCCAGAT 
CCATCGCTCA AGGCTGAAGT TTTACCAAGA ACCCGCCAAA GAGTTGTTCG CAGCTCAACA 
TCCACACCTC AAAGTACCAA ACAGGTCCCA GACTCTCCAG CGGGAGGCAC CAGCGCAGAA 
TTTAACAGCC CTCCGCGCCT CACAAGGGTC TCGCCGCCCA GTATCGACAC GTCCAGGCGC 
AGCTTCGTTC ACTCTCCTTC TGTTAAAGTG GGTAACGTCA CCCAGCAGAT AATCAGCACT 
GAACCTGGGA AAGATGTCTC TGAGCTGCCG CTTCAACTTT GGCTTTGTCC TTCTCTGGAG 
CCCATTAGCC CTACACAAAC TCAGGAAATA CTCTCGCAGC AAACCGTAGG GTCACCAAAT 
TTTCCAGAGG CTTCGCTGAA TGGGACAATG ACGGTGAAGG AGAGTCTGAC AATGACGGTG 
AAGGAGAGTC TTCCTTTGCT CAGTGAATGT GAAGGATGCG GATGTCCATT TTCAGCTCAG 
AAGCATGGAG ACACTTTGTG CTACAGATGC AGAATTAAAA GAGAGAAGAA GCGGTCTCCT 
CCAAACATTT TGTGTAGGAA GCAGAGACCA CAAAGATATG GGAAAACCGA AAATGATATT 
GTGCCTGATG GTGGTGATGA TGATGATGAA GAGTACTGGC GTTTAAAGAA GCGTAATCGG 
AGGATGTGTC AGCGGTGTGA TGCCTGCCGT CGTGATACAG ACTGTGGGAA GTGCGACTTC 
TGCATGGACA AGCCCAAGTT TGGTGGCAGT AACAAGAAAA GGCAGAAATG TCGCTTGCGT 
CAGTGCAAGT TTCAGTCAAA GCTTCAATTT CGAAATGGAA TGAAGGGAGT GCCGACTTCA 
TCTCTTTCAC CAATAAAGAA AATGATCAAA TCCAATAAAC TCCTCAAAAT AAAGAAACGT 
GGGAGACCAC GCAAAAAAAA ATTCAAAAAT GACCCATGGG AGGAGGAGGA GGAGGATTAT 
GAAGTGTCTG ATAATGATGA AGATGATCAA GAGTTGAGGA GTTCTGAGAT GAACGGCTCC 
CAGGTCAGAA AACACGGGAA ATGGAGTTAC ACTTTTAAGG AAGATGAAGA CGACGCGGCC 
TATGTTGAGG ATGTGGTTGA TGCCAACATT GAAGAGGATT CTGTTATTTT GGATAATGAA 
CGGTCAGTGA TGGCGTCTAA CGAGATGTAC AACACCACCT CTGGACTCAG TTCTCAGGGA 
TTATACTACA ATGTGTCAGG AATGCCGCTG TCGTCTCATC TGATTGGCTC AGCTCCTATC 
TGTAACAGCG GTGCAGTGGC TATGGGTGAG GTGATCGGCT CGGTGCCCAT GGCGGACGAG 
ATGAGCCAGA ATGGATTTCT TCAGATCGAG ATGGTGAAAG TGGGATCATC TCCATCACAT 
TACAGAGAAG AACAGCAGAA CACACAACAA ATGCACATTG CAGACCCTCA ACAGGAGCAC 
ACGCCGGTGA TCACTCAGAT CTTCAGTCTG GCGGGTCCAG AGACAGAAAG TGATCGAGAC 
CCAGGCTTGA TCGAGCTGTT CACGTCCCTC GGCCAGAGCG TCCTGCCGGC TCACTGGGTG 
TGTGTGATCG CCAAAGGCCC TGTCCTGCAG CTCCTGCAGT GCTCTAAACT CTCCACCATG 
GCAGACACCG TGGTCCAGAT CGAGAAAGGC TTCTTCTACC AGGTCACCGT CCAGAACCAG 
CCTCTGCTGC TCATGCACAG CCTCTACTCT CGCCATCCCA CCTGCCTGGA GAAAGTGGAG 
CATGTAGTGT CCCTCCTGCT GGATCTGGAA GCTCTGGGTG TTTGTCAGGG CTACCAAAAC 
TTTCAAATGT GCTCGCCTTG GGAGCCTAAA ATGTGTGTGC GAGCCGCACT ATGCGATTTG 
CTCATTCCTA AGGATGATGA GCATTGTGGG AAATGCTCGC AGCCGGTGGA GGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302035.1
CDS	338..2674
Translation	MTDELNELSPESEILKPEDAVPAYLNEMHPIGEDVDSDVEDDDMSCKVTVELLPDWLKPMEEEEVGVDELMSAGRINRRKTKEKPPKVKRGRGRPPKIRHGLSRSEFKKGLHADRTMKKKRVGSLMSRKRLSRKATSVPDPSLKAEVLPRTRQRVVRSSTSTPQSTKQVPDSPAGGTSAEFNSPPRLTRVSPPSIDTSRRSFVHSPSVKVGNVTQQIISTEPGKDVSELPLQLWLCPSLEPISPTQTQEILSQQTVGSPNFPEASLNGTMTVKESLTMTVKESLPLLSECEGCGCPFSAQKHGDTLCYRCRIKREKKRSPPNILCRKQRPQRYGKTENDIVPDGGDDDDEEYWRLKKRNRRMCQRCDACRRDTDCGKCDFCMDKPKFGGSNKKRQKCRLRQCKFQSKLQFRNGMKGVPTSSLSPIKKMIKSNKLLKIKKRGRPRKKKFKNDPWEEEEEDYEVSDNDEDDQELRSSEMNGSQVRKHGKWSYTFKEDEDDAAYVEDVVDANIEEDSVILDNERSVMASNEMYNTTSGLSSQGLYYNVSGMPLSSHLIGSAPICNSGAVAMGEVIGSVPMADEMSQNGFLQIEMVKVGSSPSHYREEQQNTQQMHIADPQQEHTPVITQIFSLAGPETESDRDPGLIELFTSLGQSVLPAHWVCVIAKGPVLQLLQCSKLSTMADTVVQIEKGFFYQVTVQNQPLLLMHSLYSRHPTCLEKVEHVVSLLLDLEALGVCQGYQNFQMCSPWEPKMCVRAALCDLLIPKDDEHCGKCSQPVEG

Target ORF information:

RefSeq Version	XM_009303760.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio methyl-CpG binding domain protein 1a (mbd1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303760.2

ORF Insert Sequence:

ATGACGGATG AGCTGAACGA GCTATCACCT GAATCTGAGA TCTTGAAACC AGAAGACGCT 
GTGCCAGCAT ACTTAAACGA GATGCATCCG ATCGGAGAAG ACGTTGATAG TGACGTTGAG 
GATGACGACA TGTCCTGCAA AGTGACGGTT GAGTTATTAC CTGATTGGTT GAAGCCTATG 
GAGGAGGAGG AAGTTGGTGT TGATGAGCTC ATGAGCGCTG GACGGATCAA CCGGAGAAAA 
ACTAAAGAAA AACCTCCGAA AGTAAAACGG GGCAGGGGTC GACCTCCTAA AATAAGACAT 
GGACTTAGTC GTTCAGAGTT CAAGAAAGGA CTGCATGCGG ATCGCACTAT GAAGAAAAAG 
AGGGTTGGAT CCCTAATGAG TCGCAAACGT TTATCCCGAA AAGCCACTTC CGTCCCAGAT 
CCATCGCTCA AGGCTGAAGT TTTACCAAGA ACCCGCCAAA GAGTTGTTCG CAGCTCAACA 
TCCACACCTC AAAGTACCAA ACAGGTCCCA GACTCTCCAG CGGGAGGCAC CAGCGCAGAA 
TTTAACAGCC CTCCGCGCCT CACAAGGGTC TCGCCGCCCA GTATCGACAC GTCCAGGCGC 
AGCTTCGTTC ACTCTCCTTC TGTTAAAGTG GGTAACGTCA CCCAGCAGAT AATCAGCACT 
GAACCTGGGA AAGATGTCTC TGAGCTGCCG CTTCAACTTT GGCTTTGTCC TTCTCTGGAG 
CCCATTAGCC CTACACAAAC TCAGGAAATA CTCTCGCAGC AAACCGTAGG GTCACCAAAT 
TTTCCAGAGG CTTCGCTGAA TGGGACAATG ACGGTGAAGG AGAGTCTGAC AATGACGGTG 
AAGGAGAGTC TTCCTTTGCT CAGTGAATGT GAAGGATGCG GATGTCCATT TTCAGCTCAG 
AAGCATGGAG ACACTTTGTG CTACAGATGC AGAATTAAAA GAGAGAAGAA GCGGTCTCCT 
CCAAACATTT TGTGTAGGAA GCAGAGACCA CAAAGATATG GGAAAACCGA AAATGATATT 
GTGCCTGATG GTGGTGATGA TGATGATGAA GAGTACTGGC GTTTAAAGAA GCGTAATCGG 
AGGATGTGTC AGCGGTGTGA TGCCTGCCGT CGTGATACAG ACTGTGGGAA GTGCGACTTC 
TGCATGGACA AGCCCAAGTT TGGTGGCAGT AACAAGAAAA GGCAGAAATG TCGCTTGCGT 
CAGTGCAAGT TTCAGTCAAA GCTTCAATTT CGAAATGGAA TGAAGGGAGT GCCGACTTCA 
TCTCTTTCAC CAATAAAGAA AATGATCAAA TCCAATAAAC TCCTCAAAAT AAAGAAACGT 
GGGAGACCAC GCAAAAAAAA ATTCAAAAAT GACCCATGGG AGGAGGAGGA GGAGGATTAT 
GAAGTGTCTG ATAATGATGA AGATGATCAA GAGTTGAGGA GTTCTGAGAT GAACGGCTCC 
CAGGTCAGAA AACACGGGAA ATGGAGTTAC ACTTTTAAGG AAGATGAAGA CGACGCGGCC 
TATGTTGAGG ATGTGGTTGA TGCCAACATT GAAGAGGATT CTGTTATTTT GGATAATGAA 
CGGTCAGTGA TGGCGTCTAA CGAGATGTAC AACACCACCT CTGGACTCAG TTCTCAGGGA 
TTATACTACA ATGTGTCAGG AATGCCGCTG TCGTCTCATC TGATTGGCTC AGCTCCTATC 
TGTAACAGCG GTGCAGTGGC TATGGGTGAG GTGATCGGCT CGGTGCCCAT GGCGGACGAG 
ATGAGCCAGA ATGGATTTCT TCAGATCGAG ATGGTGAAAG TGGGATCATC TCCATCACAT 
TACAGAGAAG AACAGCAGAA CACACAACAA ATGCACATTG CAGACCCTCA ACAGGAGCAC 
ACGCCGGTGA TCACTCAGAT CTTCAGTCTG GCGGGTCCAG AGACAGAAAG TGATCGAGAC 
CCAGGCTTGA TCGAGCTGTT CACGTCCCTC GGCCAGAGCG TCCTGCCGGC TCACTGGGTG 
TGTGTGATCG CCAAAGGCCC TGTCCTGCAG CTCCTGCAGT GCTCTAAACT CTCCACCATG 
GCAGACACCG TGGTCCAGAT CGAGAAAGGC TTCTTCTACC AGGTCACCGT CCAGAACCAG 
CCTCTGCTGC TCATGCACAG CCTCTACTCT CGCCATCCCA CCTGCCTGGA GAAAGTGGAG 
CATGTAGTGT CCCTCCTGCT GGATCTGGAA GCTCTGGGTG TTTGTCAGGG CTACCAAAAC 
TTTCAAATGT GCTCGCCTTG GGAGCCTAAA ATGTGTGTGC GAGCCGCACT ATGCGATTTG 
CTCATTCCTA AGGATGATGA GCATTGTGGG AAATGCTCGC AGCCGGTGGA GGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mbd1a ( XM_021478323.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333998.1 Danio rerio methyl-CpG binding domain protein 1a (mbd1a), transcript variant X3, mRNA.

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CloneID	ODa47151
Clone ID Related Accession (Same CDS sequence)	XM_021478323.1
Accession Version	XM_021478323.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	methyl-CpG binding domain protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGATG AGCTGAACGA GCTATCACCT GAATCTGAGA TCTTGAAACC AGAAGACGCT 
GTGCCAGCAT ACTTAAACGA GATGCATCCG ATCGGAGAAG ACGTTGATAG TGACGTTGAG 
GATGACGACA TGTCCTGCAA AGTGACGGTT GAGTTATTAC CTGATTGGTT GAAGCCTATG 
GAGGAGGAGG AAGTTGGTGT TGATGAGCTC ATGAGCGCTG GACGGATCAA CCGGAGAAAA 
ACTAAAGAAA AACCTCCGAA AGTAAAACGG GGCAGGGGTC GACCTCCTAA AATAAGACAT 
GGACTTAGTC GTTCAGAGTT CAAGAAAGGA CTGCATGCGG ATCGCACTAT GAAGAAAAAG 
AGGGTTGGAT CCCTAATGAG TCGCAAACGT TTATCCCGAA AAGCCACTTC CGTCCCAGAT 
CCATCGCTCA AGGCTGAAGT TTTACCAAGA ACCCGCCAAA GAGTTGTTCG CAGCTCAACA 
TCCACACCTC AAAGTACCAA ACAGGTCCCA GACTCTCCAG CGGGAGGCAC CAGCGCAGAA 
TTTAACAGCC CTCCGCGCCT CACAAGGGTC TCGCCGCCCA GTATCGACAC GTCCAGGCGC 
AGCTTCGTTC ACTCTCCTTC TGTTAAAGTG GGTAACGTCA CCCAGCAGAT AATCAGCACT 
GAACCTGGGA AAGATGTCTC TGAGCTGCCG CTTCAACTTT GGCTTTGTCC TTCTCTGGAG 
CCCATTAGCC CTACACAAAC TCAGGAAATA CTCTCGCAGC AAACCGTAGG GTCACCAAAT 
TTTCCAGAGG CTTCGCTGAA TGGGACAATG ACGGTGAAGG AGAGTCTGAC AATGACGGTG 
AAGGAGAGTC TTCCTTTGCT CAGTGAATGT GAAGGATGCG GATGTCCATT TTCAGCTCAG 
AAGCATGGAG ACACTTTGTG CTACAGATGC AGAATTAAAA GAGAGAAGAA GCGGTCTCCT 
CCAAACATTT TGTGTAGGAA GAGACCACAA AGATATGGGA AAACCGAAAA TGATATTGTG 
CCTGATGGTG GTGATGATGA TGATGAAGAG TACTGGCGTT TAAAGAAGCG TAATCGGAGG 
ATGTGTCAGC GGTGTGATGC CTGCCGTCGT GATACAGACT GTGGGAAGTG CGACTTCTGC 
ATGGACAAGC CCAAGTTTGG TGGCAGTAAC AAGAAAAGGC AGAAATGTCG CTTGCGTCAG 
TGCAAGTTTC AGTCAAAGCT TCAATTTCGA AATGGAATGA AGGGAGTGCC GACTTCATCT 
CTTTCACCAA TAAAGAAAAT GATCAAATCC AATAAACTCC TCAAAATAAA GAAACGTGGG 
AGACCACGCA AAAAAAAATT CAAAAATGAC CCATGGGAGG AGGAGGAGGA GGATTATGAA 
GTGTCTGATA ATGATGAAGA TGATCAAGAG TTGAGGAGTT CTGAGATGAA CGGCTCCCAG 
GTCAGAAAAC ACGGGAAATG GAGTTACACT TTTAAGGAAG ATGAAGACGA CGCGGCCTAT 
GTTGAGGATG TGGTTGATGC CAACATTGAA GAGGATTCTG TTATTTTGGA TAATGAACGG 
TCAGTGATGG CGTCTAACGA GATGTACAAC ACCACCTCTG GACTCAGTTC TCAGGGATTA 
TACTACAATG TGTCAGGAAT GCCGCTGTCG TCTCATCTGA TTGGCTCAGC TCCTATCTGT 
AACAGCGGTG CAGTGGCTAT GGGTGAGGTG ATCGGCTCGG TGCCCATGGC GGACGAGATG 
AGCCAGAATG GATTTCTTCA GATCGAGATG GTGAAAGTGG GATCATCTCC ATCACATTAC 
AGAGAAGAAC AGCAGAACAC ACAACAAATG CACATTGCAG ACCCTCAACA GGAGCACACG 
CCGGTGATCA CTCAGATCTT CAGTCTGGCG GGTCCAGAGA CAGAAAGTGA TCGAGACCCA 
GGCTTGATCG AGCTGTTCAC GTCCCTCGGC CAGAGCGTCC TGCCGGCTCA CTGGGTGTGT 
GTGATCGCCA AAGGCCCTGT CCTGCAGCTC CTGCAGTGCT CTAAACTCTC CACCATGGCA 
GACACCGTGG TCCAGATCGA GAAAGGCTTC TTCTACCAGG TCACCGTCCA GAACCAGCCT 
CTGCTGCTCA TGCACAGCCT CTACTCTCGC CATCCCACCT GCCTGGAGAA AGTGGAGCAT 
GTAGTGTCCC TCCTGCTGGA TCTGGAAGCT CTGGGTGTTT GTCAGGGCTA CCAAAACTTT 
CAAATGTGCT CGCCTTGGGA GCCTAAAATG TGTGTGCGAG CCGCACTATG CGATTTGCTC 
ATTCCTAAGG ATGATGAGCA TTGTGGGAAA TGCTCGCAGC CGGTGGAGGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333998.1
CDS	376..2709
Translation	MTDELNELSPESEILKPEDAVPAYLNEMHPIGEDVDSDVEDDDMSCKVTVELLPDWLKPMEEEEVGVDELMSAGRINRRKTKEKPPKVKRGRGRPPKIRHGLSRSEFKKGLHADRTMKKKRVGSLMSRKRLSRKATSVPDPSLKAEVLPRTRQRVVRSSTSTPQSTKQVPDSPAGGTSAEFNSPPRLTRVSPPSIDTSRRSFVHSPSVKVGNVTQQIISTEPGKDVSELPLQLWLCPSLEPISPTQTQEILSQQTVGSPNFPEASLNGTMTVKESLTMTVKESLPLLSECEGCGCPFSAQKHGDTLCYRCRIKREKKRSPPNILCRKRPQRYGKTENDIVPDGGDDDDEEYWRLKKRNRRMCQRCDACRRDTDCGKCDFCMDKPKFGGSNKKRQKCRLRQCKFQSKLQFRNGMKGVPTSSLSPIKKMIKSNKLLKIKKRGRPRKKKFKNDPWEEEEEDYEVSDNDEDDQELRSSEMNGSQVRKHGKWSYTFKEDEDDAAYVEDVVDANIEEDSVILDNERSVMASNEMYNTTSGLSSQGLYYNVSGMPLSSHLIGSAPICNSGAVAMGEVIGSVPMADEMSQNGFLQIEMVKVGSSPSHYREEQQNTQQMHIADPQQEHTPVITQIFSLAGPETESDRDPGLIELFTSLGQSVLPAHWVCVIAKGPVLQLLQCSKLSTMADTVVQIEKGFFYQVTVQNQPLLLMHSLYSRHPTCLEKVEHVVSLLLDLEALGVCQGYQNFQMCSPWEPKMCVRAALCDLLIPKDDEHCGKCSQPVEG

Target ORF information:

RefSeq Version	XM_021478323.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio methyl-CpG binding domain protein 1a (mbd1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478323.1

ORF Insert Sequence:

ATGACGGATG AGCTGAACGA GCTATCACCT GAATCTGAGA TCTTGAAACC AGAAGACGCT 
GTGCCAGCAT ACTTAAACGA GATGCATCCG ATCGGAGAAG ACGTTGATAG TGACGTTGAG 
GATGACGACA TGTCCTGCAA AGTGACGGTT GAGTTATTAC CTGATTGGTT GAAGCCTATG 
GAGGAGGAGG AAGTTGGTGT TGATGAGCTC ATGAGCGCTG GACGGATCAA CCGGAGAAAA 
ACTAAAGAAA AACCTCCGAA AGTAAAACGG GGCAGGGGTC GACCTCCTAA AATAAGACAT 
GGACTTAGTC GTTCAGAGTT CAAGAAAGGA CTGCATGCGG ATCGCACTAT GAAGAAAAAG 
AGGGTTGGAT CCCTAATGAG TCGCAAACGT TTATCCCGAA AAGCCACTTC CGTCCCAGAT 
CCATCGCTCA AGGCTGAAGT TTTACCAAGA ACCCGCCAAA GAGTTGTTCG CAGCTCAACA 
TCCACACCTC AAAGTACCAA ACAGGTCCCA GACTCTCCAG CGGGAGGCAC CAGCGCAGAA 
TTTAACAGCC CTCCGCGCCT CACAAGGGTC TCGCCGCCCA GTATCGACAC GTCCAGGCGC 
AGCTTCGTTC ACTCTCCTTC TGTTAAAGTG GGTAACGTCA CCCAGCAGAT AATCAGCACT 
GAACCTGGGA AAGATGTCTC TGAGCTGCCG CTTCAACTTT GGCTTTGTCC TTCTCTGGAG 
CCCATTAGCC CTACACAAAC TCAGGAAATA CTCTCGCAGC AAACCGTAGG GTCACCAAAT 
TTTCCAGAGG CTTCGCTGAA TGGGACAATG ACGGTGAAGG AGAGTCTGAC AATGACGGTG 
AAGGAGAGTC TTCCTTTGCT CAGTGAATGT GAAGGATGCG GATGTCCATT TTCAGCTCAG 
AAGCATGGAG ACACTTTGTG CTACAGATGC AGAATTAAAA GAGAGAAGAA GCGGTCTCCT 
CCAAACATTT TGTGTAGGAA GAGACCACAA AGATATGGGA AAACCGAAAA TGATATTGTG 
CCTGATGGTG GTGATGATGA TGATGAAGAG TACTGGCGTT TAAAGAAGCG TAATCGGAGG 
ATGTGTCAGC GGTGTGATGC CTGCCGTCGT GATACAGACT GTGGGAAGTG CGACTTCTGC 
ATGGACAAGC CCAAGTTTGG TGGCAGTAAC AAGAAAAGGC AGAAATGTCG CTTGCGTCAG 
TGCAAGTTTC AGTCAAAGCT TCAATTTCGA AATGGAATGA AGGGAGTGCC GACTTCATCT 
CTTTCACCAA TAAAGAAAAT GATCAAATCC AATAAACTCC TCAAAATAAA GAAACGTGGG 
AGACCACGCA AAAAAAAATT CAAAAATGAC CCATGGGAGG AGGAGGAGGA GGATTATGAA 
GTGTCTGATA ATGATGAAGA TGATCAAGAG TTGAGGAGTT CTGAGATGAA CGGCTCCCAG 
GTCAGAAAAC ACGGGAAATG GAGTTACACT TTTAAGGAAG ATGAAGACGA CGCGGCCTAT 
GTTGAGGATG TGGTTGATGC CAACATTGAA GAGGATTCTG TTATTTTGGA TAATGAACGG 
TCAGTGATGG CGTCTAACGA GATGTACAAC ACCACCTCTG GACTCAGTTC TCAGGGATTA 
TACTACAATG TGTCAGGAAT GCCGCTGTCG TCTCATCTGA TTGGCTCAGC TCCTATCTGT 
AACAGCGGTG CAGTGGCTAT GGGTGAGGTG ATCGGCTCGG TGCCCATGGC GGACGAGATG 
AGCCAGAATG GATTTCTTCA GATCGAGATG GTGAAAGTGG GATCATCTCC ATCACATTAC 
AGAGAAGAAC AGCAGAACAC ACAACAAATG CACATTGCAG ACCCTCAACA GGAGCACACG 
CCGGTGATCA CTCAGATCTT CAGTCTGGCG GGTCCAGAGA CAGAAAGTGA TCGAGACCCA 
GGCTTGATCG AGCTGTTCAC GTCCCTCGGC CAGAGCGTCC TGCCGGCTCA CTGGGTGTGT 
GTGATCGCCA AAGGCCCTGT CCTGCAGCTC CTGCAGTGCT CTAAACTCTC CACCATGGCA 
GACACCGTGG TCCAGATCGA GAAAGGCTTC TTCTACCAGG TCACCGTCCA GAACCAGCCT 
CTGCTGCTCA TGCACAGCCT CTACTCTCGC CATCCCACCT GCCTGGAGAA AGTGGAGCAT 
GTAGTGTCCC TCCTGCTGGA TCTGGAAGCT CTGGGTGTTT GTCAGGGCTA CCAAAACTTT 
CAAATGTGCT CGCCTTGGGA GCCTAAAATG TGTGTGCGAG CCGCACTATG CGATTTGCTC 
ATTCCTAAGG ATGATGAGCA TTGTGGGAAA TGCTCGCAGC CGGTGGAGGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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mbd1a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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