asic1a cDNA ORF clone, Danio rerio(zebrafish)

The following asic1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the asic1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa01186 NM_214790.2
Latest version!
Danio rerio acid-sensing (proton-gated) ion channel 1a (asic1a), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
ODa01186 XM_009303740.2
Latest version!
Danio rerio acid-sensing (proton-gated) ion channel 1a (asic1a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
ODa143721 XM_021478320.1
Latest version!
Danio rerio acid-sensing (proton-gated) ion channel 1a (asic1a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa01186
    Clone ID Related Accession (Same CDS sequence) XM_009303740.2 , NM_214790.2
    Accession Version NM_214790.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1506bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-21
    Organism Danio rerio(zebrafish)
    Product acid-sensing ion channel 1A
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC162330.1. On or before Jun 7, 2009 this sequence version replaced XM_001331737.1, NM_214790.1. ##Evidence-Data-START## Transcript exon combination :: BC162330.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAAGACAT CAGTGATGGA TCTTAAAGTG GAACCAATGG ACATTGATTT CGACCAGCCT 
    CCGCCTCTCC AAGTGTTCGC TCACACCTCA ACTCTACACG GCATCTCTCA CATCTTCTCC
    TATGAGAAGA TCACGGCTAA ATGCTGCCTC TGGGTGGTGT TTTTCCTCAG CTCTTTGACT
    TTTCTCATGT ACGTCTGCAT TGATCGCATT CAGTTCTACC TGGAATATCC TCATGTCACC
    AAGCTGGATG AGATCACCAC GCCGGTGATG GTGTTTCCGG CTGTGACCAT TTGCAATCTG
    AACTCCATTC GCTTCAGCAG GATCACGCGC AACGACCTGT ACCATGCAGG AGAGCTGCTG
    GCGCTGCTCA ACTCCAGGCA TGAAGTTCGA GAAGCTCACC TGGTTGAAGA AAGCGTGATG
    GAGGTTCTCA AGAGCAAAAC CGACTTTCGC TCTTTTAAGC CGCGACATTT CAACATGTGG
    GAGTTTTACA ACAGAACAGG ACACGACATT AAAGACATGC TGCTCTCCTG CCAGTTCAGA
    GGCTCACCAT GCCGTCCTGA AGACTTCAGT GTGGTGTTCA CACGTTATGG AAAGTGTTAC
    ACCTTTAACT CTGGTGAGAC CGGCCCGCCG CGGGTCAGCG TTAAGGGCGG TATGGGGAAC
    GGCCTGGAGA TCATGCTGGA CATTCAGCAG GATGAATATT TACCTGTTTG GGGAGAGTCA
    GACGAGTCTT CATTTGAAGC AGGAATTAAA GTCCAGATTC ACAGTCAGGA CGAGCCTCCG
    TTTATAGATC AGCTGGGCTT CGGAGTCGCT CCAGGATTCC AGACGTTTGT TTCCTGCCAA
    GAGCAGCGAC TGGTGTATCT GCCTGCGCCA TGGGGGAGCT GTAAATCCAC ACCGCCGAGC
    TCCGATTATT TCAGAGCATA CAGCATCAGC GCCTGCCGAA CCGACTGCGA GACTCGATAT
    CTGGTGGAGA ACTGCAACTG CCGCATGGTG CACATGCCTG GTGATGCACC GTACTGCACC
    CCTGTGCTTT ACAAAGAATG TGCACATCCA GCACTTGATT TCCTGGTGGA AACTGACAGC
    GACTACTGCT CGTGCGAGAC TCCGTGCAAC ATCACACGCT ACAGTAAAGA GCTGTCCTTT
    GTCAAGATCC CCAGCAAAGC CTCCGTCAAA TACCTTGCCA AAAAGTACAG CAAGTCCGAG
    AAATACATAA CTGAGAATGT TATGGTGCTG GATGTGTTTT TCGAGGCTCT GAACTATGAA
    ACCATCGAGC AGAGGAAAGC CTATGAGGTG GCTGGTCTGT TAGGGGATAT TGGAGGACAA
    ATGGGGCTTT TCATTGGAGC CAGTATTCTT ACCATTCTGG AACTGTTTGA CTACCTTTAC
    GAGGTCATGA AGTACAGGTT GTGCCGCTGC TCCAACAAGA AACATCACAA CAACAACAAC
    AACACGGATC ACAACGCTGT ATTCAGCCTG GATGATGTTA ATTGTCACGT CAGTAAATTC
    CATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_999955.1
    CDS97..1602
    Translation

    Target ORF information:

    RefSeq Version NM_214790.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio acid-sensing (proton-gated) ion channel 1a (asic1a), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_214790.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAAGACAT CAGTGATGGA TCTTAAAGTG GAACCAATGG ACATTGATTT CGACCAGCCT 
    CCGCCTCTCC AAGTGTTCGC TCACACCTCA ACTCTACACG GCATCTCTCA CATCTTCTCC
    TATGAGAAGA TCACGGCTAA ATGCTGCCTC TGGGTGGTGT TTTTCCTCAG CTCTTTGACT
    TTTCTCATGT ACGTCTGCAT TGATCGCATT CAGTTCTACC TGGAATATCC TCATGTCACC
    AAGCTGGATG AGATCACCAC GCCGGTGATG GTGTTTCCGG CTGTGACCAT TTGCAATCTG
    AACTCCATTC GCTTCAGCAG GATCACGCGC AACGACCTGT ACCATGCAGG AGAGCTGCTG
    GCGCTGCTCA ACTCCAGGCA TGAAGTTCGA GAAGCTCACC TGGTTGAAGA AAGCGTGATG
    GAGGTTCTCA AGAGCAAAAC CGACTTTCGC TCTTTTAAGC CGCGACATTT CAACATGTGG
    GAGTTTTACA ACAGAACAGG ACACGACATT AAAGACATGC TGCTCTCCTG CCAGTTCAGA
    GGCTCACCAT GCCGTCCTGA AGACTTCAGT GTGGTGTTCA CACGTTATGG AAAGTGTTAC
    ACCTTTAACT CTGGTGAGAC CGGCCCGCCG CGGGTCAGCG TTAAGGGCGG TATGGGGAAC
    GGCCTGGAGA TCATGCTGGA CATTCAGCAG GATGAATATT TACCTGTTTG GGGAGAGTCA
    GACGAGTCTT CATTTGAAGC AGGAATTAAA GTCCAGATTC ACAGTCAGGA CGAGCCTCCG
    TTTATAGATC AGCTGGGCTT CGGAGTCGCT CCAGGATTCC AGACGTTTGT TTCCTGCCAA
    GAGCAGCGAC TGGTGTATCT GCCTGCGCCA TGGGGGAGCT GTAAATCCAC ACCGCCGAGC
    TCCGATTATT TCAGAGCATA CAGCATCAGC GCCTGCCGAA CCGACTGCGA GACTCGATAT
    CTGGTGGAGA ACTGCAACTG CCGCATGGTG CACATGCCTG GTGATGCACC GTACTGCACC
    CCTGTGCTTT ACAAAGAATG TGCACATCCA GCACTTGATT TCCTGGTGGA AACTGACAGC
    GACTACTGCT CGTGCGAGAC TCCGTGCAAC ATCACACGCT ACAGTAAAGA GCTGTCCTTT
    GTCAAGATCC CCAGCAAAGC CTCCGTCAAA TACCTTGCCA AAAAGTACAG CAAGTCCGAG
    AAATACATAA CTGAGAATGT TATGGTGCTG GATGTGTTTT TCGAGGCTCT GAACTATGAA
    ACCATCGAGC AGAGGAAAGC CTATGAGGTG GCTGGTCTGT TAGGGGATAT TGGAGGACAA
    ATGGGGCTTT TCATTGGAGC CAGTATTCTT ACCATTCTGG AACTGTTTGA CTACCTTTAC
    GAGGTCATGA AGTACAGGTT GTGCCGCTGC TCCAACAAGA AACATCACAA CAACAACAAC
    AACACGGATC ACAACGCTGT ATTCAGCCTG GATGATGTTA ATTGTCACGT CAGTAAATTC
    CATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa01186
    Clone ID Related Accession (Same CDS sequence) XM_009303740.2 , NM_214790.2
    Accession Version XM_009303740.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1506bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product acid-sensing ion channel 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303740.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAAGACAT CAGTGATGGA TCTTAAAGTG GAACCAATGG ACATTGATTT CGACCAGCCT 
    CCGCCTCTCC AAGTGTTCGC TCACACCTCA ACTCTACACG GCATCTCTCA CATCTTCTCC
    TATGAGAAGA TCACGGCTAA ATGCTGCCTC TGGGTGGTGT TTTTCCTCAG CTCTTTGACT
    TTTCTCATGT ACGTCTGCAT TGATCGCATT CAGTTCTACC TGGAATATCC TCATGTCACC
    AAGCTGGATG AGATCACCAC GCCGGTGATG GTGTTTCCGG CTGTGACCAT TTGCAATCTG
    AACTCCATTC GCTTCAGCAG GATCACGCGC AACGACCTGT ACCATGCAGG AGAGCTGCTG
    GCGCTGCTCA ACTCCAGGCA TGAAGTTCGA GAAGCTCACC TGGTTGAAGA AAGCGTGATG
    GAGGTTCTCA AGAGCAAAAC CGACTTTCGC TCTTTTAAGC CGCGACATTT CAACATGTGG
    GAGTTTTACA ACAGAACAGG ACACGACATT AAAGACATGC TGCTCTCCTG CCAGTTCAGA
    GGCTCACCAT GCCGTCCTGA AGACTTCAGT GTGGTGTTCA CACGTTATGG AAAGTGTTAC
    ACCTTTAACT CTGGTGAGAC CGGCCCGCCG CGGGTCAGCG TTAAGGGCGG TATGGGGAAC
    GGCCTGGAGA TCATGCTGGA CATTCAGCAG GATGAATATT TACCTGTTTG GGGAGAGTCA
    GACGAGTCTT CATTTGAAGC AGGAATTAAA GTCCAGATTC ACAGTCAGGA CGAGCCTCCG
    TTTATAGATC AGCTGGGCTT CGGAGTCGCT CCAGGATTCC AGACGTTTGT TTCCTGCCAA
    GAGCAGCGAC TGGTGTATCT GCCTGCGCCA TGGGGGAGCT GTAAATCCAC ACCGCCGAGC
    TCCGATTATT TCAGAGCATA CAGCATCAGC GCCTGCCGAA CCGACTGCGA GACTCGATAT
    CTGGTGGAGA ACTGCAACTG CCGCATGGTG CACATGCCTG GTGATGCACC GTACTGCACC
    CCTGTGCTTT ACAAAGAATG TGCACATCCA GCACTTGATT TCCTGGTGGA AACTGACAGC
    GACTACTGCT CGTGCGAGAC TCCGTGCAAC ATCACACGCT ACAGTAAAGA GCTGTCCTTT
    GTCAAGATCC CCAGCAAAGC CTCCGTCAAA TACCTTGCCA AAAAGTACAG CAAGTCCGAG
    AAATACATAA CTGAGAATGT TATGGTGCTG GATGTGTTTT TCGAGGCTCT GAACTATGAA
    ACCATCGAGC AGAGGAAAGC CTATGAGGTG GCTGGTCTGT TAGGGGATAT TGGAGGACAA
    ATGGGGCTTT TCATTGGAGC CAGTATTCTT ACCATTCTGG AACTGTTTGA CTACCTTTAC
    GAGGTCATGA AGTACAGGTT GTGCCGCTGC TCCAACAAGA AACATCACAA CAACAACAAC
    AACACGGATC ACAACGCTGT ATTCAGCCTG GATGATGTTA ATTGTCACGT CAGTAAATTC
    CATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009302015.1
    CDS98..1603
    Translation

    Target ORF information:

    RefSeq Version XM_009303740.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio acid-sensing (proton-gated) ion channel 1a (asic1a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303740.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAAGACAT CAGTGATGGA TCTTAAAGTG GAACCAATGG ACATTGATTT CGACCAGCCT 
    CCGCCTCTCC AAGTGTTCGC TCACACCTCA ACTCTACACG GCATCTCTCA CATCTTCTCC
    TATGAGAAGA TCACGGCTAA ATGCTGCCTC TGGGTGGTGT TTTTCCTCAG CTCTTTGACT
    TTTCTCATGT ACGTCTGCAT TGATCGCATT CAGTTCTACC TGGAATATCC TCATGTCACC
    AAGCTGGATG AGATCACCAC GCCGGTGATG GTGTTTCCGG CTGTGACCAT TTGCAATCTG
    AACTCCATTC GCTTCAGCAG GATCACGCGC AACGACCTGT ACCATGCAGG AGAGCTGCTG
    GCGCTGCTCA ACTCCAGGCA TGAAGTTCGA GAAGCTCACC TGGTTGAAGA AAGCGTGATG
    GAGGTTCTCA AGAGCAAAAC CGACTTTCGC TCTTTTAAGC CGCGACATTT CAACATGTGG
    GAGTTTTACA ACAGAACAGG ACACGACATT AAAGACATGC TGCTCTCCTG CCAGTTCAGA
    GGCTCACCAT GCCGTCCTGA AGACTTCAGT GTGGTGTTCA CACGTTATGG AAAGTGTTAC
    ACCTTTAACT CTGGTGAGAC CGGCCCGCCG CGGGTCAGCG TTAAGGGCGG TATGGGGAAC
    GGCCTGGAGA TCATGCTGGA CATTCAGCAG GATGAATATT TACCTGTTTG GGGAGAGTCA
    GACGAGTCTT CATTTGAAGC AGGAATTAAA GTCCAGATTC ACAGTCAGGA CGAGCCTCCG
    TTTATAGATC AGCTGGGCTT CGGAGTCGCT CCAGGATTCC AGACGTTTGT TTCCTGCCAA
    GAGCAGCGAC TGGTGTATCT GCCTGCGCCA TGGGGGAGCT GTAAATCCAC ACCGCCGAGC
    TCCGATTATT TCAGAGCATA CAGCATCAGC GCCTGCCGAA CCGACTGCGA GACTCGATAT
    CTGGTGGAGA ACTGCAACTG CCGCATGGTG CACATGCCTG GTGATGCACC GTACTGCACC
    CCTGTGCTTT ACAAAGAATG TGCACATCCA GCACTTGATT TCCTGGTGGA AACTGACAGC
    GACTACTGCT CGTGCGAGAC TCCGTGCAAC ATCACACGCT ACAGTAAAGA GCTGTCCTTT
    GTCAAGATCC CCAGCAAAGC CTCCGTCAAA TACCTTGCCA AAAAGTACAG CAAGTCCGAG
    AAATACATAA CTGAGAATGT TATGGTGCTG GATGTGTTTT TCGAGGCTCT GAACTATGAA
    ACCATCGAGC AGAGGAAAGC CTATGAGGTG GCTGGTCTGT TAGGGGATAT TGGAGGACAA
    ATGGGGCTTT TCATTGGAGC CAGTATTCTT ACCATTCTGG AACTGTTTGA CTACCTTTAC
    GAGGTCATGA AGTACAGGTT GTGCCGCTGC TCCAACAAGA AACATCACAA CAACAACAAC
    AACACGGATC ACAACGCTGT ATTCAGCCTG GATGATGTTA ATTGTCACGT CAGTAAATTC
    CATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa143721
    Clone ID Related Accession (Same CDS sequence) XM_021478320.1
    Accession Version XM_021478320.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1845bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product acid-sensing ion channel 1A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGTCCAAAA ACACAACAAT ACAACGTTTT CTTATACTAC TACGGACTCT AATAATTCTG 
    GGGGTTCACG CCCACAAACT CCCTCCCACT CTCTCTCTCT CTCTCCTCTT TCTCGCACAC
    ACATACTCAC GATCCTCTGC TTCAGCCATT AGAGCTTCAG AATCATCACA CGGGAGGATC
    CAGCGCTCGA GAACCATCAT CTCCTCAAAG ATAACATGGC TTTCCGTCAG TGTGTCGGAT
    GGACAGCGGC TCTCTGCAAA TGTTCGGATA ACTGTCGTCT GTTTTCCTGT CCAGCATCTT
    TCTGAAGTGC TCCAGCTTTC CCAGTGGAGG AACAAGTCAA TGAAGACATC AGTGATGGAT
    CTTAAAGTGG AACCAATGGA CATTGATTTC GACCAGCCTC CGCCTCTCCA AGTGTTCGCT
    CACACCTCAA CTCTACACGG CATCTCTCAC ATCTTCTCCT ATGAGAAGAT CACGGCTAAA
    TGCTGCCTCT GGGTGGTGTT TTTCCTCAGC TCTTTGACTT TTCTCATGTA CGTCTGCATT
    GATCGCATTC AGTTCTACCT GGAATATCCT CATGTCACCA AGCTGGATGA GATCACCACG
    CCGGTGATGG TGTTTCCGGC TGTGACCATT TGCAATCTGA ACTCCATTCG CTTCAGCAGG
    ATCACGCGCA ACGACCTGTA CCATGCAGGA GAGCTGCTGG CGCTGCTCAA CTCCAGGCAT
    GAAGTTCGAG AAGCTCACCT GGTTGAAGAA AGCGTGATGG AGGTTCTCAA GAGCAAAACC
    GACTTTCGCT CTTTTAAGCC GCGACATTTC AACATGTGGG AGTTTTACAA CAGAACAGGA
    CACGACATTA AAGACATGCT GCTCTCCTGC CAGTTCAGAG GCTCACCATG CCGTCCTGAA
    GACTTCAGTG TGGTGTTCAC ACGTTATGGA AAGTGTTACA CCTTTAACTC TGGTGAGACC
    GGCCCGCCGC GGGTCAGCGT TAAGGGCGGT ATGGGGAACG GCCTGGAGAT CATGCTGGAC
    ATTCAGCAGG ATGAATATTT ACCTGTTTGG GGAGAGTCAG ACGAGTCTTC ATTTGAAGCA
    GGAATTAAAG TCCAGATTCA CAGTCAGGAC GAGCCTCCGT TTATAGATCA GCTGGGCTTC
    GGAGTCGCTC CAGGATTCCA GACGTTTGTT TCCTGCCAAG AGCAGCGACT GGTGTATCTG
    CCTGCGCCAT GGGGGAGCTG TAAATCCACA CCGCCGAGCT CCGATTATTT CAGAGCATAC
    AGCATCAGCG CCTGCCGAAC CGACTGCGAG ACTCGATATC TGGTGGAGAA CTGCAACTGC
    CGCATGGTGC ACATGCCTGG TGATGCACCG TACTGCACCC CTGTGCTTTA CAAAGAATGT
    GCACATCCAG CACTTGATTT CCTGGTGGAA ACTGACAGCG ACTACTGCTC GTGCGAGACT
    CCGTGCAACA TCACACGCTA CAGTAAAGAG CTGTCCTTTG TCAAGATCCC CAGCAAAGCC
    TCCGTCAAAT ACCTTGCCAA AAAGTACAGC AAGTCCGAGA AATACATAAC TGAGAATGTT
    ATGGTGCTGG ATGTGTTTTT CGAGGCTCTG AACTATGAAA CCATCGAGCA GAGGAAAGCC
    TATGAGGTGG CTGGTCTGTT AGGGGATATT GGAGGACAAA TGGGGCTTTT CATTGGAGCC
    AGTATTCTTA CCATTCTGGA ACTGTTTGAC TACCTTTACG AGGTCATGAA GTACAGGTTG
    TGCCGCTGCT CCAACAAGAA ACATCACAAC AACAACAACA ACACGGATCA CAACGCTGTA
    TTCAGCCTGG ATGATGTTAA TTGTCACGTC AGTAAATTCC ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333995.1
    CDS16..1860
    Translation

    Target ORF information:

    RefSeq Version XM_021478320.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio acid-sensing (proton-gated) ion channel 1a (asic1a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021478320.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGTCCAAAA ACACAACAAT ACAACGTTTT CTTATACTAC TACGGACTCT AATAATTCTG 
    GGGGTTCACG CCCACAAACT CCCTCCCACT CTCTCTCTCT CTCTCCTCTT TCTCGCACAC
    ACATACTCAC GATCCTCTGC TTCAGCCATT AGAGCTTCAG AATCATCACA CGGGAGGATC
    CAGCGCTCGA GAACCATCAT CTCCTCAAAG ATAACATGGC TTTCCGTCAG TGTGTCGGAT
    GGACAGCGGC TCTCTGCAAA TGTTCGGATA ACTGTCGTCT GTTTTCCTGT CCAGCATCTT
    TCTGAAGTGC TCCAGCTTTC CCAGTGGAGG AACAAGTCAA TGAAGACATC AGTGATGGAT
    CTTAAAGTGG AACCAATGGA CATTGATTTC GACCAGCCTC CGCCTCTCCA AGTGTTCGCT
    CACACCTCAA CTCTACACGG CATCTCTCAC ATCTTCTCCT ATGAGAAGAT CACGGCTAAA
    TGCTGCCTCT GGGTGGTGTT TTTCCTCAGC TCTTTGACTT TTCTCATGTA CGTCTGCATT
    GATCGCATTC AGTTCTACCT GGAATATCCT CATGTCACCA AGCTGGATGA GATCACCACG
    CCGGTGATGG TGTTTCCGGC TGTGACCATT TGCAATCTGA ACTCCATTCG CTTCAGCAGG
    ATCACGCGCA ACGACCTGTA CCATGCAGGA GAGCTGCTGG CGCTGCTCAA CTCCAGGCAT
    GAAGTTCGAG AAGCTCACCT GGTTGAAGAA AGCGTGATGG AGGTTCTCAA GAGCAAAACC
    GACTTTCGCT CTTTTAAGCC GCGACATTTC AACATGTGGG AGTTTTACAA CAGAACAGGA
    CACGACATTA AAGACATGCT GCTCTCCTGC CAGTTCAGAG GCTCACCATG CCGTCCTGAA
    GACTTCAGTG TGGTGTTCAC ACGTTATGGA AAGTGTTACA CCTTTAACTC TGGTGAGACC
    GGCCCGCCGC GGGTCAGCGT TAAGGGCGGT ATGGGGAACG GCCTGGAGAT CATGCTGGAC
    ATTCAGCAGG ATGAATATTT ACCTGTTTGG GGAGAGTCAG ACGAGTCTTC ATTTGAAGCA
    GGAATTAAAG TCCAGATTCA CAGTCAGGAC GAGCCTCCGT TTATAGATCA GCTGGGCTTC
    GGAGTCGCTC CAGGATTCCA GACGTTTGTT TCCTGCCAAG AGCAGCGACT GGTGTATCTG
    CCTGCGCCAT GGGGGAGCTG TAAATCCACA CCGCCGAGCT CCGATTATTT CAGAGCATAC
    AGCATCAGCG CCTGCCGAAC CGACTGCGAG ACTCGATATC TGGTGGAGAA CTGCAACTGC
    CGCATGGTGC ACATGCCTGG TGATGCACCG TACTGCACCC CTGTGCTTTA CAAAGAATGT
    GCACATCCAG CACTTGATTT CCTGGTGGAA ACTGACAGCG ACTACTGCTC GTGCGAGACT
    CCGTGCAACA TCACACGCTA CAGTAAAGAG CTGTCCTTTG TCAAGATCCC CAGCAAAGCC
    TCCGTCAAAT ACCTTGCCAA AAAGTACAGC AAGTCCGAGA AATACATAAC TGAGAATGTT
    ATGGTGCTGG ATGTGTTTTT CGAGGCTCTG AACTATGAAA CCATCGAGCA GAGGAAAGCC
    TATGAGGTGG CTGGTCTGTT AGGGGATATT GGAGGACAAA TGGGGCTTTT CATTGGAGCC
    AGTATTCTTA CCATTCTGGA ACTGTTTGAC TACCTTTACG AGGTCATGAA GTACAGGTTG
    TGCCGCTGCT CCAACAAGAA ACATCACAAC AACAACAACA ACACGGATCA CAACGCTGTA
    TTCAGCCTGG ATGATGTTAA TTGTCACGTC AGTAAATTCC ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.