efemp2b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	efemp2b
Entrez Gene ID	572703
Full Name	EGF containing fibulin-like extracellular matrix protein 2b
Synonyms	CB257,fbln4,fc56c09,id:ibd2923,sb:cb257,wu:fc56c09.x1
General protein information	Preferred Names EGF containing fibulin-like extracellular matrix protein 2b Names EGF containing fibulin-like extracellular matrix protein 2b fibulin 4
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001160358.1	NP_001153830.1	EGF containing fibulin-like extracellular matrix protein 2b precursor
XM_009295330.2	XP_009293605.1	EGF containing fibulin-like extracellular matrix protein 2b isoform X1
XM_017352820.1	XP_017208309.1	EGF containing fibulin-like extracellular matrix protein 2b isoform X1
NM_001160358.2	NP_001153830.1	EGF containing fibulin extracellular matrix protein 2b precursor

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	efemp2a	NP_001008587.1
Danio rerio (zebrafish)	efemp2b	NP_001153830.1
Danio rerio (zebrafish)	LOC100537581	XP_003200899.1

Gene Ontology
Bibliography

Related articles in PubMed

Genomic organization and evolution of olfactory receptors and trace amine-associated receptors in channel catfish, Ictalurus punctatus.
Gao S, Liu S, Yao J, Li N, Yuan Z, Zhou T, Li Q, Liu Z
Biochimica et biophysica acta1861(3)644-651(2017 03)

Grass carp Ctenopharyngodon idella Fibulin-4 as a potential interacting partner for grass carp reovirus outer capsid proteins.
Yu F, Wang H, Liu W, Lu L
Fish & shellfish immunology48169-74(2016 Jan)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Recurrent Mutations in the Basic Domain of TWIST2 Cause Ablepharon Macrostomia and Barber-Say Syndromes.
Marchegiani S, Davis T, Tessadori F, van Haaften G, Brancati F, Hoischen A, Huang H, Valkanas E, Pusey B, Schanze D, Venselaar H, Vulto-van Silfhout AT, Wolfe LA, Tifft CJ, Zerfas PM, Zambruno G, Kariminejad A, Sabbagh-Kermani F, Lee J, Tsokos MG, Lee CC, Ferraz V, da Silva EM, Stevens CA, Roche N, Bartsch O, Farndon P, Bermejo-Sanchez E, Brooks BP, Maduro V, Dallapiccola B, Ramos FJ, Chung HY, Le Caignec C, Martins F, Jacyk WK, Mazzanti L, Brunner HG, Bakkers J, Lin S, Malicdan MC, Boerkoel CF, Gahl WA, de Vries BB, van Haelst MM, Zenker M, Markello TC
American journal of human genetics97(1)99-110(2015 Jul)

Identification of direct T-box target genes in the developing zebrafish mesoderm.
Garnett AT, Han TM, Gilchrist MJ, Smith JC, Eisen MB, Wardle FC, Amacher SL
Development (Cambridge, England)136(5)749-60(2009 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following efemp2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the efemp2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa25780	NM_001160358.2 Latest version!	Danio rerio EGF containing fibulin extracellular matrix protein 2b (efemp2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa25780	XM_009295330.2 Latest version!	Danio rerio EGF containing fibulin-like extracellular matrix protein 2b (efemp2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa25780	XM_017352820.1 Latest version!	Danio rerio EGF containing fibulin-like extracellular matrix protein 2b (efemp2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
View Old Accession Versions >>
ODa25780	NM_001160358.1	Danio rerio EGF containing fibulin-like extracellular matrix protein 2b (efemp2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

efemp2b ( NM_001160358.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001153830.1 Danio rerio EGF containing fibulin-like extracellular matrix protein 2b (efemp2b), mRNA.

Price & Availability↑

CloneID	ODa25780
Clone ID Related Accession (Same CDS sequence)	NM_001160358.1 , XM_009295330.2 , XM_017352820.1 , NM_001160358.2
Accession Version	NM_001160358.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-25
Organism	Danio rerio(zebrafish)
Product	EGF containing fibulin-like extracellular matrix protein 2b precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC159249.1. On May 22, 2009 this sequence version replaced XM_702050.2. ##Evidence-Data-START## Transcript exon combination :: BC159249.1, AY427783.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGAAGGCCA CATATGTGTT TGTTTGCGTG GGTCTGGCTC TTCTTCCTGG TACAATCTGC 
CAGTCTCCTG CTGAAAGTGA AGCTTACACA GACTGTACAG ATGGCTATGA GTGGGACGAG 
CAGACTCAAC TCTGTCGAGA CATAAATGAG TGTGAGACCA TTCTGGAGGC CTGTCGAGGA 
GAAATGAAGT GCTTCAATCA TTATGGTGGT TACCTGTGTC TTCCTCGCTC CTCCTACGTG 
ATCACTGCAC CTGAGACTGC CAGCCAATCA GAGCCCAATG TGCCTGTGCC AGTGCCAGAA 
AACGAGGCCT TCAACCCATG TCCACTTGGC TACAGGGCCC AAGGAGAGTC CTGCGTGGAT 
ATTGATGAGT GTGAGCTGAA CATGCATGAC TGCCAGCCCA GCCAGGAATG TATCAACACA 
GTGGGCACCT TCACCTGTCA GTGTCCTGAC GGCTACAGCA AGATTGGCAT AGAGTGTGTG 
GATATCGACG AGTGCAGGTA CAGGTACTGT CAGCATCGCT GTGTGAACGT GCCAGGATCT 
TTCTCTTGTG AGTGTGAACC AGGCTTTCAG CTGGCTGGAA ACAACCGATC CTGTGTTGAT 
GTAAATGAGT GTGAAATGGG AGCACCTTGT CAGCAAAGGT GTTACAACAG TTACGGGACG 
TTCCTGTGCC GCTGTGATCA AGGCTATGAC CTGGGACCTG ATGGCTACTC ATGCAATGAC 
ATCAACGAGT GCAGCTACTC CAGTATCTTG TGCCAGTATC AGTGTGTGAA TGAGCCTGGA 
AAATTCTCCT GTGTTTGTCC AGAAGGCTAC GAGCTACTTG GATCTCGACT CTGCCAGGAT 
GTGAATGAGT GTGAGACTGG CAGTCATCAG TGTGGAGAAG GACAGGTCTG TGTGAACATC 
CATGGAGGAC ATCAGTGTGT GAGCGCCAAC CGCTGCCAGG AGCCCTACAT CCAGGTCTCA 
GAGAATCGTT GTATTTGTCC GGTAGTGAAG CCGGAGTGCC GTGATCTGCC CTTCTCTATT 
GTGCATCGAT ACATGAGCAT CACGTCTGAA CGCTCCGTTC CCTCCGACAT CTTCCAGATT 
CAGGCCACCA GTGTCTATCC TGGAGCCTAC AACTCCTTCC GCATCCGATC TGGAGACCAA 
AATGAGGAAT TCTACATACG GCAAATCAAC AACATCAGTG CTATGCTGGT TTTGGCGCGG 
ACCGTGACGG GGCCGAAGGA GTACGTCCTG GACCTGGAGA TGCAGTCGGT AAATCCACTG 
ATGAACTACC AGACCAGCTC AGTCCTGCGC CTCGCCGTTT ACATCGGCCC TCATGTCTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153830.1
CDS	52..1374
Translation	MKATYVFVCVGLALLPGTICQSPAESEAYTDCTDGYEWDEQTQLCRDINECETILEACRGEMKCFNHYGGYLCLPRSSYVITAPETASQSEPNVPVPVPENEAFNPCPLGYRAQGESCVDIDECELNMHDCQPSQECINTVGTFTCQCPDGYSKIGIECVDIDECRYRYCQHRCVNVPGSFSCECEPGFQLAGNNRSCVDVNECEMGAPCQQRCYNSYGTFLCRCDQGYDLGPDGYSCNDINECSYSSILCQYQCVNEPGKFSCVCPEGYELLGSRLCQDVNECETGSHQCGEGQVCVNIHGGHQCVSANRCQEPYIQVSENRCICPVVKPECRDLPFSIVHRYMSITSERSVPSDIFQIQATSVYPGAYNSFRIRSGDQNEEFYIRQINNISAMLVLARTVTGPKEYVLDLEMQSVNPLMNYQTSSVLRLAVYIGPHVF

Target ORF information:

RefSeq Version	NM_001160358.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio EGF containing fibulin-like extracellular matrix protein 2b (efemp2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001160358.1

ORF Insert Sequence:

ATGAAGGCCA CATATGTGTT TGTTTGCGTG GGTCTGGCTC TTCTTCCTGG TACAATCTGC 
CAGTCTCCTG CTGAAAGTGA AGCTTACACA GACTGTACAG ATGGCTATGA GTGGGACGAG 
CAGACTCAAC TCTGTCGAGA CATAAATGAG TGTGAGACCA TTCTGGAGGC CTGTCGAGGA 
GAAATGAAGT GCTTCAATCA TTATGGTGGT TACCTGTGTC TTCCTCGCTC CTCCTACGTG 
ATCACTGCAC CTGAGACTGC CAGCCAATCA GAGCCCAATG TGCCTGTGCC AGTGCCAGAA 
AACGAGGCCT TCAACCCATG TCCACTTGGC TACAGGGCCC AAGGAGAGTC CTGCGTGGAT 
ATTGATGAGT GTGAGCTGAA CATGCATGAC TGCCAGCCCA GCCAGGAATG TATCAACACA 
GTGGGCACCT TCACCTGTCA GTGTCCTGAC GGCTACAGCA AGATTGGCAT AGAGTGTGTG 
GATATCGACG AGTGCAGGTA CAGGTACTGT CAGCATCGCT GTGTGAACGT GCCAGGATCT 
TTCTCTTGTG AGTGTGAACC AGGCTTTCAG CTGGCTGGAA ACAACCGATC CTGTGTTGAT 
GTAAATGAGT GTGAAATGGG AGCACCTTGT CAGCAAAGGT GTTACAACAG TTACGGGACG 
TTCCTGTGCC GCTGTGATCA AGGCTATGAC CTGGGACCTG ATGGCTACTC ATGCAATGAC 
ATCAACGAGT GCAGCTACTC CAGTATCTTG TGCCAGTATC AGTGTGTGAA TGAGCCTGGA 
AAATTCTCCT GTGTTTGTCC AGAAGGCTAC GAGCTACTTG GATCTCGACT CTGCCAGGAT 
GTGAATGAGT GTGAGACTGG CAGTCATCAG TGTGGAGAAG GACAGGTCTG TGTGAACATC 
CATGGAGGAC ATCAGTGTGT GAGCGCCAAC CGCTGCCAGG AGCCCTACAT CCAGGTCTCA 
GAGAATCGTT GTATTTGTCC GGTAGTGAAG CCGGAGTGCC GTGATCTGCC CTTCTCTATT 
GTGCATCGAT ACATGAGCAT CACGTCTGAA CGCTCCGTTC CCTCCGACAT CTTCCAGATT 
CAGGCCACCA GTGTCTATCC TGGAGCCTAC AACTCCTTCC GCATCCGATC TGGAGACCAA 
AATGAGGAAT TCTACATACG GCAAATCAAC AACATCAGTG CTATGCTGGT TTTGGCGCGG 
ACCGTGACGG GGCCGAAGGA GTACGTCCTG GACCTGGAGA TGCAGTCGGT AAATCCACTG 
ATGAACTACC AGACCAGCTC AGTCCTGCGC CTCGCCGTTT ACATCGGCCC TCATGTCTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

efemp2b ( NM_001160358.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001153830.1 Danio rerio EGF containing fibulin extracellular matrix protein 2b (efemp2b), mRNA.

Price & Availability↑

CloneID	ODa25780
Clone ID Related Accession (Same CDS sequence)	NM_001160358.1 , XM_009295330.2 , XM_017352820.1 , NM_001160358.2
Accession Version	NM_001160358.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-21
Organism	Danio rerio(zebrafish)
Product	EGF containing fibulin extracellular matrix protein 2b precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR388166.7. On Aug 22, 2018 this sequence version replaced NM_001160358.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AY427783.1, BC159249.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGAAGGCCA CATATGTGTT TGTTTGCGTG GGTCTGGCTC TTCTTCCTGG TACAATCTGC 
CAGTCTCCTG CTGAAAGTGA AGCTTACACA GACTGTACAG ATGGCTATGA GTGGGACGAG 
CAGACTCAAC TCTGTCGAGA CATAAATGAG TGTGAGACCA TTCTGGAGGC CTGTCGAGGA 
GAAATGAAGT GCTTCAATCA TTATGGTGGT TACCTGTGTC TTCCTCGCTC CTCCTACGTG 
ATCACTGCAC CTGAGACTGC CAGCCAATCA GAGCCCAATG TGCCTGTGCC AGTGCCAGAA 
AACGAGGCCT TCAACCCATG TCCACTTGGC TACAGGGCCC AAGGAGAGTC CTGCGTGGAT 
ATTGATGAGT GTGAGCTGAA CATGCATGAC TGCCAGCCCA GCCAGGAATG TATCAACACA 
GTGGGCACCT TCACCTGTCA GTGTCCTGAC GGCTACAGCA AGATTGGCAT AGAGTGTGTG 
GATATCGACG AGTGCAGGTA CAGGTACTGT CAGCATCGCT GTGTGAACGT GCCAGGATCT 
TTCTCTTGTG AGTGTGAACC AGGCTTTCAG CTGGCTGGAA ACAACCGATC CTGTGTTGAT 
GTAAATGAGT GTGAAATGGG AGCACCTTGT CAGCAAAGGT GTTACAACAG TTACGGGACG 
TTCCTGTGCC GCTGTGATCA AGGCTATGAC CTGGGACCTG ATGGCTACTC ATGCAATGAC 
ATCAACGAGT GCAGCTACTC CAGTATCTTG TGCCAGTATC AGTGTGTGAA TGAGCCTGGA 
AAATTCTCCT GTGTTTGTCC AGAAGGCTAC GAGCTACTTG GATCTCGACT CTGCCAGGAT 
GTGAATGAGT GTGAGACTGG CAGTCATCAG TGTGGAGAAG GACAGGTCTG TGTGAACATC 
CATGGAGGAC ATCAGTGTGT GAGCGCCAAC CGCTGCCAGG AGCCCTACAT CCAGGTCTCA 
GAGAATCGTT GTATTTGTCC GGTAGTGAAG CCGGAGTGCC GTGATCTGCC CTTCTCTATT 
GTGCATCGAT ACATGAGCAT CACGTCTGAA CGCTCCGTTC CCTCCGACAT CTTCCAGATT 
CAGGCCACCA GTGTCTATCC TGGAGCCTAC AACTCCTTCC GCATCCGATC TGGAGACCAA 
AATGAGGAAT TCTACATACG GCAAATCAAC AACATCAGTG CTATGCTGGT TTTGGCGCGG 
ACCGTGACGG GGCCGAAGGA GTACGTCCTG GACCTGGAGA TGCAGTCGGT AAATCCACTG 
ATGAACTACC AGACCAGCTC AGTCCTGCGC CTCGCCGTTT ACATCGGCCC TCATGTCTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153830.1
CDS	112..1434
Translation	MKATYVFVCVGLALLPGTICQSPAESEAYTDCTDGYEWDEQTQLCRDINECETILEACRGEMKCFNHYGGYLCLPRSSYVITAPETASQSEPNVPVPVPENEAFNPCPLGYRAQGESCVDIDECELNMHDCQPSQECINTVGTFTCQCPDGYSKIGIECVDIDECRYRYCQHRCVNVPGSFSCECEPGFQLAGNNRSCVDVNECEMGAPCQQRCYNSYGTFLCRCDQGYDLGPDGYSCNDINECSYSSILCQYQCVNEPGKFSCVCPEGYELLGSRLCQDVNECETGSHQCGEGQVCVNIHGGHQCVSANRCQEPYIQVSENRCICPVVKPECRDLPFSIVHRYMSITSERSVPSDIFQIQATSVYPGAYNSFRIRSGDQNEEFYIRQINNISAMLVLARTVTGPKEYVLDLEMQSVNPLMNYQTSSVLRLAVYIGPHVF

Target ORF information:

RefSeq Version	NM_001160358.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio EGF containing fibulin extracellular matrix protein 2b (efemp2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001160358.2

ORF Insert Sequence:

ATGAAGGCCA CATATGTGTT TGTTTGCGTG GGTCTGGCTC TTCTTCCTGG TACAATCTGC 
CAGTCTCCTG CTGAAAGTGA AGCTTACACA GACTGTACAG ATGGCTATGA GTGGGACGAG 
CAGACTCAAC TCTGTCGAGA CATAAATGAG TGTGAGACCA TTCTGGAGGC CTGTCGAGGA 
GAAATGAAGT GCTTCAATCA TTATGGTGGT TACCTGTGTC TTCCTCGCTC CTCCTACGTG 
ATCACTGCAC CTGAGACTGC CAGCCAATCA GAGCCCAATG TGCCTGTGCC AGTGCCAGAA 
AACGAGGCCT TCAACCCATG TCCACTTGGC TACAGGGCCC AAGGAGAGTC CTGCGTGGAT 
ATTGATGAGT GTGAGCTGAA CATGCATGAC TGCCAGCCCA GCCAGGAATG TATCAACACA 
GTGGGCACCT TCACCTGTCA GTGTCCTGAC GGCTACAGCA AGATTGGCAT AGAGTGTGTG 
GATATCGACG AGTGCAGGTA CAGGTACTGT CAGCATCGCT GTGTGAACGT GCCAGGATCT 
TTCTCTTGTG AGTGTGAACC AGGCTTTCAG CTGGCTGGAA ACAACCGATC CTGTGTTGAT 
GTAAATGAGT GTGAAATGGG AGCACCTTGT CAGCAAAGGT GTTACAACAG TTACGGGACG 
TTCCTGTGCC GCTGTGATCA AGGCTATGAC CTGGGACCTG ATGGCTACTC ATGCAATGAC 
ATCAACGAGT GCAGCTACTC CAGTATCTTG TGCCAGTATC AGTGTGTGAA TGAGCCTGGA 
AAATTCTCCT GTGTTTGTCC AGAAGGCTAC GAGCTACTTG GATCTCGACT CTGCCAGGAT 
GTGAATGAGT GTGAGACTGG CAGTCATCAG TGTGGAGAAG GACAGGTCTG TGTGAACATC 
CATGGAGGAC ATCAGTGTGT GAGCGCCAAC CGCTGCCAGG AGCCCTACAT CCAGGTCTCA 
GAGAATCGTT GTATTTGTCC GGTAGTGAAG CCGGAGTGCC GTGATCTGCC CTTCTCTATT 
GTGCATCGAT ACATGAGCAT CACGTCTGAA CGCTCCGTTC CCTCCGACAT CTTCCAGATT 
CAGGCCACCA GTGTCTATCC TGGAGCCTAC AACTCCTTCC GCATCCGATC TGGAGACCAA 
AATGAGGAAT TCTACATACG GCAAATCAAC AACATCAGTG CTATGCTGGT TTTGGCGCGG 
ACCGTGACGG GGCCGAAGGA GTACGTCCTG GACCTGGAGA TGCAGTCGGT AAATCCACTG 
ATGAACTACC AGACCAGCTC AGTCCTGCGC CTCGCCGTTT ACATCGGCCC TCATGTCTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

efemp2b ( XM_009295330.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293605.1 Danio rerio EGF containing fibulin-like extracellular matrix protein 2b (efemp2b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa25780
Clone ID Related Accession (Same CDS sequence)	NM_001160358.1 , XM_009295330.2 , XM_017352820.1 , NM_001160358.2
Accession Version	XM_009295330.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	EGF containing fibulin-like extracellular matrix protein 2b isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295330.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCCA CATATGTGTT TGTTTGCGTG GGTCTGGCTC TTCTTCCTGG TACAATCTGC 
CAGTCTCCTG CTGAAAGTGA AGCTTACACA GACTGTACAG ATGGCTATGA GTGGGACGAG 
CAGACTCAAC TCTGTCGAGA CATAAATGAG TGTGAGACCA TTCTGGAGGC CTGTCGAGGA 
GAAATGAAGT GCTTCAATCA TTATGGTGGT TACCTGTGTC TTCCTCGCTC CTCCTACGTG 
ATCACTGCAC CTGAGACTGC CAGCCAATCA GAGCCCAATG TGCCTGTGCC AGTGCCAGAA 
AACGAGGCCT TCAACCCATG TCCACTTGGC TACAGGGCCC AAGGAGAGTC CTGCGTGGAT 
ATTGATGAGT GTGAGCTGAA CATGCATGAC TGCCAGCCCA GCCAGGAATG TATCAACACA 
GTGGGCACCT TCACCTGTCA GTGTCCTGAC GGCTACAGCA AGATTGGCAT AGAGTGTGTG 
GATATCGACG AGTGCAGGTA CAGGTACTGT CAGCATCGCT GTGTGAACGT GCCAGGATCT 
TTCTCTTGTG AGTGTGAACC AGGCTTTCAG CTGGCTGGAA ACAACCGATC CTGTGTTGAT 
GTAAATGAGT GTGAAATGGG AGCACCTTGT CAGCAAAGGT GTTACAACAG TTACGGGACG 
TTCCTGTGCC GCTGTGATCA AGGCTATGAC CTGGGACCTG ATGGCTACTC ATGCAATGAC 
ATCAACGAGT GCAGCTACTC CAGTATCTTG TGCCAGTATC AGTGTGTGAA TGAGCCTGGA 
AAATTCTCCT GTGTTTGTCC AGAAGGCTAC GAGCTACTTG GATCTCGACT CTGCCAGGAT 
GTGAATGAGT GTGAGACTGG CAGTCATCAG TGTGGAGAAG GACAGGTCTG TGTGAACATC 
CATGGAGGAC ATCAGTGTGT GAGCGCCAAC CGCTGCCAGG AGCCCTACAT CCAGGTCTCA 
GAGAATCGTT GTATTTGTCC GGTAGTGAAG CCGGAGTGCC GTGATCTGCC CTTCTCTATT 
GTGCATCGAT ACATGAGCAT CACGTCTGAA CGCTCCGTTC CCTCCGACAT CTTCCAGATT 
CAGGCCACCA GTGTCTATCC TGGAGCCTAC AACTCCTTCC GCATCCGATC TGGAGACCAA 
AATGAGGAAT TCTACATACG GCAAATCAAC AACATCAGTG CTATGCTGGT TTTGGCGCGG 
ACCGTGACGG GGCCGAAGGA GTACGTCCTG GACCTGGAGA TGCAGTCGGT AAATCCACTG 
ATGAACTACC AGACCAGCTC AGTCCTGCGC CTCGCCGTTT ACATCGGCCC TCATGTCTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293605.1
CDS	2330..3652
Translation	MKATYVFVCVGLALLPGTICQSPAESEAYTDCTDGYEWDEQTQLCRDINECETILEACRGEMKCFNHYGGYLCLPRSSYVITAPETASQSEPNVPVPVPENEAFNPCPLGYRAQGESCVDIDECELNMHDCQPSQECINTVGTFTCQCPDGYSKIGIECVDIDECRYRYCQHRCVNVPGSFSCECEPGFQLAGNNRSCVDVNECEMGAPCQQRCYNSYGTFLCRCDQGYDLGPDGYSCNDINECSYSSILCQYQCVNEPGKFSCVCPEGYELLGSRLCQDVNECETGSHQCGEGQVCVNIHGGHQCVSANRCQEPYIQVSENRCICPVVKPECRDLPFSIVHRYMSITSERSVPSDIFQIQATSVYPGAYNSFRIRSGDQNEEFYIRQINNISAMLVLARTVTGPKEYVLDLEMQSVNPLMNYQTSSVLRLAVYIGPHVF

Target ORF information:

RefSeq Version	XM_009295330.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio EGF containing fibulin-like extracellular matrix protein 2b (efemp2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295330.2

ORF Insert Sequence:

ATGAAGGCCA CATATGTGTT TGTTTGCGTG GGTCTGGCTC TTCTTCCTGG TACAATCTGC 
CAGTCTCCTG CTGAAAGTGA AGCTTACACA GACTGTACAG ATGGCTATGA GTGGGACGAG 
CAGACTCAAC TCTGTCGAGA CATAAATGAG TGTGAGACCA TTCTGGAGGC CTGTCGAGGA 
GAAATGAAGT GCTTCAATCA TTATGGTGGT TACCTGTGTC TTCCTCGCTC CTCCTACGTG 
ATCACTGCAC CTGAGACTGC CAGCCAATCA GAGCCCAATG TGCCTGTGCC AGTGCCAGAA 
AACGAGGCCT TCAACCCATG TCCACTTGGC TACAGGGCCC AAGGAGAGTC CTGCGTGGAT 
ATTGATGAGT GTGAGCTGAA CATGCATGAC TGCCAGCCCA GCCAGGAATG TATCAACACA 
GTGGGCACCT TCACCTGTCA GTGTCCTGAC GGCTACAGCA AGATTGGCAT AGAGTGTGTG 
GATATCGACG AGTGCAGGTA CAGGTACTGT CAGCATCGCT GTGTGAACGT GCCAGGATCT 
TTCTCTTGTG AGTGTGAACC AGGCTTTCAG CTGGCTGGAA ACAACCGATC CTGTGTTGAT 
GTAAATGAGT GTGAAATGGG AGCACCTTGT CAGCAAAGGT GTTACAACAG TTACGGGACG 
TTCCTGTGCC GCTGTGATCA AGGCTATGAC CTGGGACCTG ATGGCTACTC ATGCAATGAC 
ATCAACGAGT GCAGCTACTC CAGTATCTTG TGCCAGTATC AGTGTGTGAA TGAGCCTGGA 
AAATTCTCCT GTGTTTGTCC AGAAGGCTAC GAGCTACTTG GATCTCGACT CTGCCAGGAT 
GTGAATGAGT GTGAGACTGG CAGTCATCAG TGTGGAGAAG GACAGGTCTG TGTGAACATC 
CATGGAGGAC ATCAGTGTGT GAGCGCCAAC CGCTGCCAGG AGCCCTACAT CCAGGTCTCA 
GAGAATCGTT GTATTTGTCC GGTAGTGAAG CCGGAGTGCC GTGATCTGCC CTTCTCTATT 
GTGCATCGAT ACATGAGCAT CACGTCTGAA CGCTCCGTTC CCTCCGACAT CTTCCAGATT 
CAGGCCACCA GTGTCTATCC TGGAGCCTAC AACTCCTTCC GCATCCGATC TGGAGACCAA 
AATGAGGAAT TCTACATACG GCAAATCAAC AACATCAGTG CTATGCTGGT TTTGGCGCGG 
ACCGTGACGG GGCCGAAGGA GTACGTCCTG GACCTGGAGA TGCAGTCGGT AAATCCACTG 
ATGAACTACC AGACCAGCTC AGTCCTGCGC CTCGCCGTTT ACATCGGCCC TCATGTCTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

efemp2b ( XM_017352820.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208309.1 Danio rerio EGF containing fibulin-like extracellular matrix protein 2b (efemp2b), transcript variant X1, mRNA.

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CloneID	ODa25780
Clone ID Related Accession (Same CDS sequence)	NM_001160358.1 , XM_009295330.2 , XM_017352820.1 , NM_001160358.2
Accession Version	XM_017352820.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	EGF containing fibulin-like extracellular matrix protein 2b isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCCA CATATGTGTT TGTTTGCGTG GGTCTGGCTC TTCTTCCTGG TACAATCTGC 
CAGTCTCCTG CTGAAAGTGA AGCTTACACA GACTGTACAG ATGGCTATGA GTGGGACGAG 
CAGACTCAAC TCTGTCGAGA CATAAATGAG TGTGAGACCA TTCTGGAGGC CTGTCGAGGA 
GAAATGAAGT GCTTCAATCA TTATGGTGGT TACCTGTGTC TTCCTCGCTC CTCCTACGTG 
ATCACTGCAC CTGAGACTGC CAGCCAATCA GAGCCCAATG TGCCTGTGCC AGTGCCAGAA 
AACGAGGCCT TCAACCCATG TCCACTTGGC TACAGGGCCC AAGGAGAGTC CTGCGTGGAT 
ATTGATGAGT GTGAGCTGAA CATGCATGAC TGCCAGCCCA GCCAGGAATG TATCAACACA 
GTGGGCACCT TCACCTGTCA GTGTCCTGAC GGCTACAGCA AGATTGGCAT AGAGTGTGTG 
GATATCGACG AGTGCAGGTA CAGGTACTGT CAGCATCGCT GTGTGAACGT GCCAGGATCT 
TTCTCTTGTG AGTGTGAACC AGGCTTTCAG CTGGCTGGAA ACAACCGATC CTGTGTTGAT 
GTAAATGAGT GTGAAATGGG AGCACCTTGT CAGCAAAGGT GTTACAACAG TTACGGGACG 
TTCCTGTGCC GCTGTGATCA AGGCTATGAC CTGGGACCTG ATGGCTACTC ATGCAATGAC 
ATCAACGAGT GCAGCTACTC CAGTATCTTG TGCCAGTATC AGTGTGTGAA TGAGCCTGGA 
AAATTCTCCT GTGTTTGTCC AGAAGGCTAC GAGCTACTTG GATCTCGACT CTGCCAGGAT 
GTGAATGAGT GTGAGACTGG CAGTCATCAG TGTGGAGAAG GACAGGTCTG TGTGAACATC 
CATGGAGGAC ATCAGTGTGT GAGCGCCAAC CGCTGCCAGG AGCCCTACAT CCAGGTCTCA 
GAGAATCGTT GTATTTGTCC GGTAGTGAAG CCGGAGTGCC GTGATCTGCC CTTCTCTATT 
GTGCATCGAT ACATGAGCAT CACGTCTGAA CGCTCCGTTC CCTCCGACAT CTTCCAGATT 
CAGGCCACCA GTGTCTATCC TGGAGCCTAC AACTCCTTCC GCATCCGATC TGGAGACCAA 
AATGAGGAAT TCTACATACG GCAAATCAAC AACATCAGTG CTATGCTGGT TTTGGCGCGG 
ACCGTGACGG GGCCGAAGGA GTACGTCCTG GACCTGGAGA TGCAGTCGGT AAATCCACTG 
ATGAACTACC AGACCAGCTC AGTCCTGCGC CTCGCCGTTT ACATCGGCCC TCATGTCTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208309.1
CDS	2375..3697
Translation	MKATYVFVCVGLALLPGTICQSPAESEAYTDCTDGYEWDEQTQLCRDINECETILEACRGEMKCFNHYGGYLCLPRSSYVITAPETASQSEPNVPVPVPENEAFNPCPLGYRAQGESCVDIDECELNMHDCQPSQECINTVGTFTCQCPDGYSKIGIECVDIDECRYRYCQHRCVNVPGSFSCECEPGFQLAGNNRSCVDVNECEMGAPCQQRCYNSYGTFLCRCDQGYDLGPDGYSCNDINECSYSSILCQYQCVNEPGKFSCVCPEGYELLGSRLCQDVNECETGSHQCGEGQVCVNIHGGHQCVSANRCQEPYIQVSENRCICPVVKPECRDLPFSIVHRYMSITSERSVPSDIFQIQATSVYPGAYNSFRIRSGDQNEEFYIRQINNISAMLVLARTVTGPKEYVLDLEMQSVNPLMNYQTSSVLRLAVYIGPHVF

Target ORF information:

RefSeq Version	XM_017352820.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio EGF containing fibulin-like extracellular matrix protein 2b (efemp2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352820.1

ORF Insert Sequence:

ATGAAGGCCA CATATGTGTT TGTTTGCGTG GGTCTGGCTC TTCTTCCTGG TACAATCTGC 
CAGTCTCCTG CTGAAAGTGA AGCTTACACA GACTGTACAG ATGGCTATGA GTGGGACGAG 
CAGACTCAAC TCTGTCGAGA CATAAATGAG TGTGAGACCA TTCTGGAGGC CTGTCGAGGA 
GAAATGAAGT GCTTCAATCA TTATGGTGGT TACCTGTGTC TTCCTCGCTC CTCCTACGTG 
ATCACTGCAC CTGAGACTGC CAGCCAATCA GAGCCCAATG TGCCTGTGCC AGTGCCAGAA 
AACGAGGCCT TCAACCCATG TCCACTTGGC TACAGGGCCC AAGGAGAGTC CTGCGTGGAT 
ATTGATGAGT GTGAGCTGAA CATGCATGAC TGCCAGCCCA GCCAGGAATG TATCAACACA 
GTGGGCACCT TCACCTGTCA GTGTCCTGAC GGCTACAGCA AGATTGGCAT AGAGTGTGTG 
GATATCGACG AGTGCAGGTA CAGGTACTGT CAGCATCGCT GTGTGAACGT GCCAGGATCT 
TTCTCTTGTG AGTGTGAACC AGGCTTTCAG CTGGCTGGAA ACAACCGATC CTGTGTTGAT 
GTAAATGAGT GTGAAATGGG AGCACCTTGT CAGCAAAGGT GTTACAACAG TTACGGGACG 
TTCCTGTGCC GCTGTGATCA AGGCTATGAC CTGGGACCTG ATGGCTACTC ATGCAATGAC 
ATCAACGAGT GCAGCTACTC CAGTATCTTG TGCCAGTATC AGTGTGTGAA TGAGCCTGGA 
AAATTCTCCT GTGTTTGTCC AGAAGGCTAC GAGCTACTTG GATCTCGACT CTGCCAGGAT 
GTGAATGAGT GTGAGACTGG CAGTCATCAG TGTGGAGAAG GACAGGTCTG TGTGAACATC 
CATGGAGGAC ATCAGTGTGT GAGCGCCAAC CGCTGCCAGG AGCCCTACAT CCAGGTCTCA 
GAGAATCGTT GTATTTGTCC GGTAGTGAAG CCGGAGTGCC GTGATCTGCC CTTCTCTATT 
GTGCATCGAT ACATGAGCAT CACGTCTGAA CGCTCCGTTC CCTCCGACAT CTTCCAGATT 
CAGGCCACCA GTGTCTATCC TGGAGCCTAC AACTCCTTCC GCATCCGATC TGGAGACCAA 
AATGAGGAAT TCTACATACG GCAAATCAAC AACATCAGTG CTATGCTGGT TTTGGCGCGG 
ACCGTGACGG GGCCGAAGGA GTACGTCCTG GACCTGGAGA TGCAGTCGGT AAATCCACTG 
ATGAACTACC AGACCAGCTC AGTCCTGCGC CTCGCCGTTT ACATCGGCCC TCATGTCTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genomic organization and evolution of olfactory receptors and trace amine-associated receptors in channel catfish, Ictalurus punctatus.
Biochimica et biophysica acta1861(3)644-651(2017 03)
Gao S,Liu S,Yao J,Li N,Yuan Z,Zhou T,Li Q,Liu Z

Grass carp Ctenopharyngodon idella Fibulin-4 as a potential interacting partner for grass carp reovirus outer capsid proteins.
Fish & shellfish immunology48169-74(2016 Jan)
Yu F,Wang H,Liu W,Lu L

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Recurrent Mutations in the Basic Domain of TWIST2 Cause Ablepharon Macrostomia and Barber-Say Syndromes.
American journal of human genetics97(1)99-110(2015 Jul)
Marchegiani S,Davis T,Tessadori F,van Haaften G,Brancati F,Hoischen A,Huang H,Valkanas E,Pusey B,Schanze D,Venselaar H,Vulto-van Silfhout AT,Wolfe LA,Tifft CJ,Zerfas PM,Zambruno G,Kariminejad A,Sabbagh-Kermani F,Lee J,Tsokos MG,Lee CC,Ferraz V,da Silva EM,Stevens CA,Roche N,Bartsch O,Farndon P,Bermejo-Sanchez E,Brooks BP,Maduro V,Dallapiccola B,Ramos FJ,Chung HY,Le Caignec C,Martins F,Jacyk WK,Mazzanti L,Brunner HG,Bakkers J,Lin S,Malicdan MC,Boerkoel CF,Gahl WA,de Vries BB,van Haelst MM,Zenker M,Markello TC

Identification of direct T-box target genes in the developing zebrafish mesoderm.
Development (Cambridge, England)136(5)749-60(2009 Mar)
Garnett AT,Han TM,Gilchrist MJ,Smith JC,Eisen MB,Wardle FC,Amacher SL

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efemp2b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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