REV1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	REV1
Entrez Gene ID	570043
Full Name	REV1, polymerase (DNA directed)
Synonyms	si:dkey-57a22.7
General protein information	Preferred Names REV1, polymerase (DNA directed) Names DNA repair protein REV1
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017357714.1	XP_017213203.1	DNA repair protein REV1 isoform X4
XM_009304637.2	XP_009302912.1	DNA repair protein REV1 isoform X2
NM_001123300.1	NP_001116772.1	DNA repair protein REV1
XM_017357714.2	XP_017213203.1	DNA repair protein REV1 isoform X3
XM_017357713.1	XP_017213202.1	DNA repair protein REV1 isoform X3
XM_005167585.4	XP_005167642.1	DNA repair protein REV1 isoform X1
XM_009304637.3	XP_009302912.1	DNA repair protein REV1 isoform X2
XM_005167585.3	XP_005167642.1	DNA repair protein REV1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	REV1	XP_001160264.1
Canis lupus familiaris (dog)	REV1	XP_005626074.1
Drosophila melanogaster (fruit fly)	Rev1	NP_612047.1
	REV1	XP_002799407.1
Bos taurus (cattle)	REV1	XP_003586659.2
Mus musculus (house mouse)	Rev1	NP_062516.2
Rattus norvegicus (Norway rat)	Rev1	NP_001101683.1
Homo sapiens (human)	REV1	NP_001032961.1
Gallus gallus (chicken)	REV1	NP_001025982.2
Danio rerio (zebrafish)	rev1	NP_001116772.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005939	XP_315969.4
Xenopus tropicalis (tropical clawed frog)	rev1	XP_002937904.2

Gene Ontology
Bibliography

Related articles in PubMed

Identification of target genes downstream of semaphorin6A/PlexinA2 signaling in zebrafish.
Emerson SE, St Clair RM, Waldron AL, Bruno SR, Duong A, Driscoll HE, Ballif BA, McFarlane S, Ebert AM
Developmental dynamics : an official publication of the American Association of Anatomists246(7)539-549(2017 Jul)

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Huang HT, Kathrein KL, Barton A, Gitlin Z, Huang YH, Ward TP, Hofmann O, Dibiase A, Song A, Tyekucheva S, Hide W, Zhou Y, Zon LI
Nature cell biology15(12)1516-25(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following REV1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the REV1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa32372	NM_001123300.1 Latest version!	Danio rerio REV1 DNA directed polymerase (rev1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ODa15099	XM_005167585.4 Latest version!	Danio rerio REV1, polymerase (DNA directed) (rev1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ODa45890	XM_009304637.3 Latest version!	Danio rerio REV1, polymerase (DNA directed) (rev1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ODa60270	XM_017357713.1 Latest version!	Danio rerio REV1, polymerase (DNA directed) (rev1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ODa60271	XM_017357714.2 Latest version!	Danio rerio REV1, polymerase (DNA directed) (rev1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
View Old Accession Versions >>
ODa60271	XM_017357714.1	Danio rerio REV1, polymerase (DNA directed) (rev1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ODa45890	XM_009304637.2	Danio rerio REV1, polymerase (DNA directed) (rev1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ODa15099	XM_005167585.3	Danio rerio REV1, polymerase (DNA directed) (rev1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

REV1 ( NM_001123300.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001116772.1 Danio rerio REV1 DNA directed polymerase (rev1), mRNA.

Price & Availability↑

CloneID	ODa32372
Clone ID Related Accession (Same CDS sequence)	NM_001123300.1
Accession Version	NM_001123300.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-31
Organism	Danio rerio(zebrafish)
Product	DNA repair protein REV1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR392332.8 and CR536604.6. On Apr 11, 2008 this sequence version replaced XM_693472.2. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA4476772, SAMEA4476776 [ECO:0000350] ##Evidence-Data-END##

ATGAGTCGAG ATGGATGGAG GAAGAAAGCC AGTGAAAATG ATGGGTGGGG AGGACAGGGA 
GGTTACATGG CTGCAAAAGT CTCTAAGCTT GAGGAGCAGT TTCAGAAGGA TGCCCCGAGA 
GAGCAGAAGA AAGATGGAAA GTCCTCCTGC ATCTTCAGCG GTGTGGCCAT CTATGTCAAT 
GGATACACAG ATCCCAGTGC GGCTGAGTTA CGCAGGCTTA TGATGTTGCA TGGTGGGCAA 
TTTCATCTGT ATTACTCTCG CTCCAAAACC ACGCACATCA TTGCCACCAA CCTGCCCAAC 
TTCAAAATAC AAGAGCTCAA AGGAGAGAAA GTAGTGCGGC CAGAGTGGAT CATAGACAGT 
ATAAAAGCAG GTCGCCAACT ATCATATATT CAGTATCAGC TCTATGCGAA GCAGAAAGGA 
TTAAATTTCA CCAGAGTTTC TGGAATAGAA GGCCAGGACC CGTCATCCAA CCATGGACCA 
GACATCAAGC CTCAGCCGAG CCATACTCTA TCTAAAGACG TCCTCAAGTC TACTCAGGCT 
AACCACATGG CTAACTACAC TGAGAAAACC GATCACCAGC TGGATATCAG GCTCAGAAAT 
GGTTTGCATA GCATTATCTC TGAGGTAGAT GCAGAGAAAT CGAGAGTCAA TGGGATTCAT 
GATGATGATG ATGATGATGA CATCGCCTGT TCCATTTTGC CAAGAGGCTC ACAAGACACT 
TTGTTAACTA ACGGTCATGT ACACCCTGTG AATGGTGCCT TAAAGCCTCA GGACTTTGCA 
GACCATCATC CTCATGTTGT TGACAAAGCG TCCAATCAGT CTCACCTCCC ACAGGACAAA 
TACAGGGAGG GTGAGGAGCC CTGCTGCTCA TATACTGAAT CAAAAGCTAA TTCCCATCTG 
AGGTCCTTAA TATCAGCAGC CAGTCCTGCA AAGCAGCCTG GTCCTCACAA TATGCAAACG 
GACCTCCATC AAAAACCAAC ATCCAATTCA GGAACTTTAG GTGCTCCTCA GAAGGCCAGT 
CTGCATGATC AGACCACAGT CCGGCTAAAT GGGAGTTATC ACGTGACCTC AAACTCCTCC 
ACTCTGGCAA ACTCTAGTAA CCAGTCAGGA AAATCCTCTG CAGAGGCTGG AATAATATCC 
GAGTTCTTTT CTCACTCAAG GCTGCATCAC ATTTCTACGT GGCGCAATGA GTTCTCGGAG 
TATGTAAACA GCCTGCAGAG CAGACGACGA GCAGCAGGTG GAGCTGTTTT CTCTGGAAGA 
GAGAAGCTGA AGAAGCTCAA AGCTAACTGC AATTCAGTCT CTCACTTTGA CCCAGGTTCC 
TTGATGGCTG CTCCTCAGGT GCGTCAGTCA TGTGTTCTCC ATGTTGATAT GGACTGCTTC 
TTTGTCTCTG TGGGGATTAG ACACCGACCA GATCTTATAG GGAAACCAGT TGCGGTGACT 
AGTAATCGTG GTCCAGGCCG TGTTGCTCAG CGCCCTGGTG CAAACCCTCA GCTTGAGTTT 
CAGTATTACC AGAATAAACA GAAACATTAC AGGAAAGAGA AGACAGATGG GGATCTTGAG 
ATGACCCCAT CTCCACAGGA TGGTGAAGTT CCCAGTAATG GAGTGCATGA AGACCTGGCT 
GCCCTTTCTA TGGCAGAGAT TGCCTCTTGT AGTTACGAGG CCAGGCAGGC TGGTGTGCGA 
AATGGCATGT TCTTTGGTAG GGCCAAGCAG CTGTGTCCAG ATCTGCAGTC TGTTCCATAT 
GACTTCCATG CTTATAAAGA GGTGGCTCTG GCCATGTATG AGATCCTGGC CAGTTACACT 
CATAACATTG AGGCGCTGAG CTGTGACGAG GCACTGGTTG ATGCCACAGC TCTTCTGGTT 
GAGCTGGGGG TGTCTCCTGA TGAACTGGCA CGGTCCATCC GTGAAGACAT AAAGGAGAAG 
ACTGGCTGCT GTGCCTCAGT TGGAATGAGT TCCAACATCC TACTTGCTCG GATGGCAACC 
CGTAAGGCTA AACCCAAAGG GCAGTATTTA CTCAGATCAG AGGAAGTGGA TGATTTTATT 
AGAGACCAGC CTGTGTCCAG TTTACCTGGT GTGGGTCGCT CAATGAGCTC CAAGTTGACC 
TCTCTAGGAG TGAGTACCTG CGGTGACCTG CAGCAGTTGT CCCTGTCTCA ACTGCAGAGG 
GAGTTTGGGC CACGGACGGG ACAGACGCTC TTTCGCTTTT GCAGGGGACT AGATGACAGA 
CCTGTGCGCA GTGAGAAAGA GAGAAAGTCT GTGTCTGCGG AGATGAACTA CAACATTCGG 
TTCACACAGG TTGAGGAGGC GGAGTCTTTC CTCAATAATC TCTCTATGGA GGTACAGAAG 
AGGTTAGAGG GGGCGGGGCT TAGAGGTCGT CGGGTTACAC TGAAGGTCAT GATGCGGAAA 
CCAGGTGCGC CGGTAGAACC AGCCAAATAT GGTGGTCATG GGATATGTGA CAACTTTGCC 
AGGTCTGTTT TACTAGCTCA GCCCACAGAC AGTGGTCGAG TGATTGCATC AGAGGCCATT 
AAGCTGTTTC ATGCCATGAA GCTGAATGTG AAGGACATGA GGGGAGTGGG GCTGCAGGTG 
CAGCAGCTGG ATGGATCACA TGCAGACCCT TCAGGACAAG GGCCCTCTCG TGGCCGATCA 
ATCAGAGACC TGCTTCTGGC CAAACAGTCT GCCCACAGCC CCAGCAAGGA GTCTCCATCA 
CAAGATGGTT TAATCAGCGC TTCGTCATCC AGAGCTTTCT CCTCCAATCA GAAGAGAGTT 
ACCCCTGCAT TATCACCGCC ACCTTCTACA TCATCATCAT CATCACCACC ACCCTCCTTC 
ACCATTATAG ATCCAATGCC TGGAACAAGT AAAGGAGATT ATCACCATCC ACACACCCCC 
AACCACGTCA GGGCCTGCCT AAACATCAGC ATCGAGGTGC CATCTCCCTC TCAGGTGGAC 
CCATCTGTTT TAAAAGCTTT ACCTATAGAC ATAAGGAGAC AAGTAGAGGA AACCTGGAGA 
TACAGAGAGG AGCAGCCCAG CACTTCTTCT CATTACTCAA CACCACCTCG ACCACCCTCC 
ACTCCACCTC CAGGACCTTC ACTTGTTCTG CAGCTCCCAA ACCAGCCTGG TCAACCATGC 
ACAACGGGCA TTATATTAGA GCTCCCGGAC TTCTCCCAGG TTGACCCAGA TGTGTTTGCA 
GCACTCCCTA GAGAGCTTCA GGAAGAGTTG TGTTCTGCTT ACAGAAATAA GGGAAATGCT 
CAAGCTCAAG CCAGCACTGT AGTGGAACAA AAAAACTCAT TTCCTCAACT GAAGCAGCCT 
GCTGTAGGGA AATTAAAGCG CCGCTACAAA AAAAGAAACA CTAGTCCTGC CAAAAATGGT 
TCAAGTCCAC TAAAAAAAAT GTTCCCTGGG AACAGCCCTG CCAAAACCAG CCCCTCCAAA 
ACTCTTCCTC TGCCACTCAG AGAACAAGAC CTCAAATGCC CGTCCTCTCT GAGCACAGAT 
GTCCCAGAGA CACTACCCAA ACCCATTCCT CGGCCAGCCC CAACCCTTGC AGGAGCGCAT 
GAATTCAGTG AGATAAGAAC GCTGCTGCGT GAATGGGTCA CCACCATCTC AGAACCAATG 
GAGGAGGACA TTTTACAGGT GGTGAAGTAC TGCACAGAAC TAGTGGAGGA CAAAGACCTG 
GAGAAACTCT ACCTTGTCAT CAAGTACATG AAGAGGCTCA TGCAGCAGTC CTCAGAGTCA 
GTGTGGAGCA TGGCCTTTGA CTTTGTCCTG GACAATGTGC AGGTGGTGGT ACAGCAAACC 
TACGGCAGCA CTTTGAAGAT CACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001116772.1
CDS	1..3807
Translation	MSRDGWRKKASENDGWGGQGGYMAAKVSKLEEQFQKDAPREQKKDGKSSCIFSGVAIYVNGYTDPSAAELRRLMMLHGGQFHLYYSRSKTTHIIATNLPNFKIQELKGEKVVRPEWIIDSIKAGRQLSYIQYQLYAKQKGLNFTRVSGIEGQDPSSNHGPDIKPQPSHTLSKDVLKSTQANHMANYTEKTDHQLDIRLRNGLHSIISEVDAEKSRVNGIHDDDDDDDIACSILPRGSQDTLLTNGHVHPVNGALKPQDFADHHPHVVDKASNQSHLPQDKYREGEEPCCSYTESKANSHLRSLISAASPAKQPGPHNMQTDLHQKPTSNSGTLGAPQKASLHDQTTVRLNGSYHVTSNSSTLANSSNQSGKSSAEAGIISEFFSHSRLHHISTWRNEFSEYVNSLQSRRRAAGGAVFSGREKLKKLKANCNSVSHFDPGSLMAAPQVRQSCVLHVDMDCFFVSVGIRHRPDLIGKPVAVTSNRGPGRVAQRPGANPQLEFQYYQNKQKHYRKEKTDGDLEMTPSPQDGEVPSNGVHEDLAALSMAEIASCSYEARQAGVRNGMFFGRAKQLCPDLQSVPYDFHAYKEVALAMYEILASYTHNIEALSCDEALVDATALLVELGVSPDELARSIREDIKEKTGCCASVGMSSNILLARMATRKAKPKGQYLLRSEEVDDFIRDQPVSSLPGVGRSMSSKLTSLGVSTCGDLQQLSLSQLQREFGPRTGQTLFRFCRGLDDRPVRSEKERKSVSAEMNYNIRFTQVEEAESFLNNLSMEVQKRLEGAGLRGRRVTLKVMMRKPGAPVEPAKYGGHGICDNFARSVLLAQPTDSGRVIASEAIKLFHAMKLNVKDMRGVGLQVQQLDGSHADPSGQGPSRGRSIRDLLLAKQSAHSPSKESPSQDGLISASSSRAFSSNQKRVTPALSPPPSTSSSSSPPPSFTIIDPMPGTSKGDYHHPHTPNHVRACLNISIEVPSPSQVDPSVLKALPIDIRRQVEETWRYREEQPSTSSHYSTPPRPPSTPPPGPSLVLQLPNQPGQPCTTGIILELPDFSQVDPDVFAALPRELQEELCSAYRNKGNAQAQASTVVEQKNSFPQLKQPAVGKLKRRYKKRNTSPAKNGSSPLKKMFPGNSPAKTSPSKTLPLPLREQDLKCPSSLSTDVPETLPKPIPRPAPTLAGAHEFSEIRTLLREWVTTISEPMEEDILQVVKYCTELVEDKDLEKLYLVIKYMKRLMQQSSESVWSMAFDFVLDNVQVVVQQTYGSTLKIT

Target ORF information:

RefSeq Version	NM_001123300.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio REV1 DNA directed polymerase (rev1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001123300.1

ORF Insert Sequence:

ATGAGTCGAG ATGGATGGAG GAAGAAAGCC AGTGAAAATG ATGGGTGGGG AGGACAGGGA 
GGTTACATGG CTGCAAAAGT CTCTAAGCTT GAGGAGCAGT TTCAGAAGGA TGCCCCGAGA 
GAGCAGAAGA AAGATGGAAA GTCCTCCTGC ATCTTCAGCG GTGTGGCCAT CTATGTCAAT 
GGATACACAG ATCCCAGTGC GGCTGAGTTA CGCAGGCTTA TGATGTTGCA TGGTGGGCAA 
TTTCATCTGT ATTACTCTCG CTCCAAAACC ACGCACATCA TTGCCACCAA CCTGCCCAAC 
TTCAAAATAC AAGAGCTCAA AGGAGAGAAA GTAGTGCGGC CAGAGTGGAT CATAGACAGT 
ATAAAAGCAG GTCGCCAACT ATCATATATT CAGTATCAGC TCTATGCGAA GCAGAAAGGA 
TTAAATTTCA CCAGAGTTTC TGGAATAGAA GGCCAGGACC CGTCATCCAA CCATGGACCA 
GACATCAAGC CTCAGCCGAG CCATACTCTA TCTAAAGACG TCCTCAAGTC TACTCAGGCT 
AACCACATGG CTAACTACAC TGAGAAAACC GATCACCAGC TGGATATCAG GCTCAGAAAT 
GGTTTGCATA GCATTATCTC TGAGGTAGAT GCAGAGAAAT CGAGAGTCAA TGGGATTCAT 
GATGATGATG ATGATGATGA CATCGCCTGT TCCATTTTGC CAAGAGGCTC ACAAGACACT 
TTGTTAACTA ACGGTCATGT ACACCCTGTG AATGGTGCCT TAAAGCCTCA GGACTTTGCA 
GACCATCATC CTCATGTTGT TGACAAAGCG TCCAATCAGT CTCACCTCCC ACAGGACAAA 
TACAGGGAGG GTGAGGAGCC CTGCTGCTCA TATACTGAAT CAAAAGCTAA TTCCCATCTG 
AGGTCCTTAA TATCAGCAGC CAGTCCTGCA AAGCAGCCTG GTCCTCACAA TATGCAAACG 
GACCTCCATC AAAAACCAAC ATCCAATTCA GGAACTTTAG GTGCTCCTCA GAAGGCCAGT 
CTGCATGATC AGACCACAGT CCGGCTAAAT GGGAGTTATC ACGTGACCTC AAACTCCTCC 
ACTCTGGCAA ACTCTAGTAA CCAGTCAGGA AAATCCTCTG CAGAGGCTGG AATAATATCC 
GAGTTCTTTT CTCACTCAAG GCTGCATCAC ATTTCTACGT GGCGCAATGA GTTCTCGGAG 
TATGTAAACA GCCTGCAGAG CAGACGACGA GCAGCAGGTG GAGCTGTTTT CTCTGGAAGA 
GAGAAGCTGA AGAAGCTCAA AGCTAACTGC AATTCAGTCT CTCACTTTGA CCCAGGTTCC 
TTGATGGCTG CTCCTCAGGT GCGTCAGTCA TGTGTTCTCC ATGTTGATAT GGACTGCTTC 
TTTGTCTCTG TGGGGATTAG ACACCGACCA GATCTTATAG GGAAACCAGT TGCGGTGACT 
AGTAATCGTG GTCCAGGCCG TGTTGCTCAG CGCCCTGGTG CAAACCCTCA GCTTGAGTTT 
CAGTATTACC AGAATAAACA GAAACATTAC AGGAAAGAGA AGACAGATGG GGATCTTGAG 
ATGACCCCAT CTCCACAGGA TGGTGAAGTT CCCAGTAATG GAGTGCATGA AGACCTGGCT 
GCCCTTTCTA TGGCAGAGAT TGCCTCTTGT AGTTACGAGG CCAGGCAGGC TGGTGTGCGA 
AATGGCATGT TCTTTGGTAG GGCCAAGCAG CTGTGTCCAG ATCTGCAGTC TGTTCCATAT 
GACTTCCATG CTTATAAAGA GGTGGCTCTG GCCATGTATG AGATCCTGGC CAGTTACACT 
CATAACATTG AGGCGCTGAG CTGTGACGAG GCACTGGTTG ATGCCACAGC TCTTCTGGTT 
GAGCTGGGGG TGTCTCCTGA TGAACTGGCA CGGTCCATCC GTGAAGACAT AAAGGAGAAG 
ACTGGCTGCT GTGCCTCAGT TGGAATGAGT TCCAACATCC TACTTGCTCG GATGGCAACC 
CGTAAGGCTA AACCCAAAGG GCAGTATTTA CTCAGATCAG AGGAAGTGGA TGATTTTATT 
AGAGACCAGC CTGTGTCCAG TTTACCTGGT GTGGGTCGCT CAATGAGCTC CAAGTTGACC 
TCTCTAGGAG TGAGTACCTG CGGTGACCTG CAGCAGTTGT CCCTGTCTCA ACTGCAGAGG 
GAGTTTGGGC CACGGACGGG ACAGACGCTC TTTCGCTTTT GCAGGGGACT AGATGACAGA 
CCTGTGCGCA GTGAGAAAGA GAGAAAGTCT GTGTCTGCGG AGATGAACTA CAACATTCGG 
TTCACACAGG TTGAGGAGGC GGAGTCTTTC CTCAATAATC TCTCTATGGA GGTACAGAAG 
AGGTTAGAGG GGGCGGGGCT TAGAGGTCGT CGGGTTACAC TGAAGGTCAT GATGCGGAAA 
CCAGGTGCGC CGGTAGAACC AGCCAAATAT GGTGGTCATG GGATATGTGA CAACTTTGCC 
AGGTCTGTTT TACTAGCTCA GCCCACAGAC AGTGGTCGAG TGATTGCATC AGAGGCCATT 
AAGCTGTTTC ATGCCATGAA GCTGAATGTG AAGGACATGA GGGGAGTGGG GCTGCAGGTG 
CAGCAGCTGG ATGGATCACA TGCAGACCCT TCAGGACAAG GGCCCTCTCG TGGCCGATCA 
ATCAGAGACC TGCTTCTGGC CAAACAGTCT GCCCACAGCC CCAGCAAGGA GTCTCCATCA 
CAAGATGGTT TAATCAGCGC TTCGTCATCC AGAGCTTTCT CCTCCAATCA GAAGAGAGTT 
ACCCCTGCAT TATCACCGCC ACCTTCTACA TCATCATCAT CATCACCACC ACCCTCCTTC 
ACCATTATAG ATCCAATGCC TGGAACAAGT AAAGGAGATT ATCACCATCC ACACACCCCC 
AACCACGTCA GGGCCTGCCT AAACATCAGC ATCGAGGTGC CATCTCCCTC TCAGGTGGAC 
CCATCTGTTT TAAAAGCTTT ACCTATAGAC ATAAGGAGAC AAGTAGAGGA AACCTGGAGA 
TACAGAGAGG AGCAGCCCAG CACTTCTTCT CATTACTCAA CACCACCTCG ACCACCCTCC 
ACTCCACCTC CAGGACCTTC ACTTGTTCTG CAGCTCCCAA ACCAGCCTGG TCAACCATGC 
ACAACGGGCA TTATATTAGA GCTCCCGGAC TTCTCCCAGG TTGACCCAGA TGTGTTTGCA 
GCACTCCCTA GAGAGCTTCA GGAAGAGTTG TGTTCTGCTT ACAGAAATAA GGGAAATGCT 
CAAGCTCAAG CCAGCACTGT AGTGGAACAA AAAAACTCAT TTCCTCAACT GAAGCAGCCT 
GCTGTAGGGA AATTAAAGCG CCGCTACAAA AAAAGAAACA CTAGTCCTGC CAAAAATGGT 
TCAAGTCCAC TAAAAAAAAT GTTCCCTGGG AACAGCCCTG CCAAAACCAG CCCCTCCAAA 
ACTCTTCCTC TGCCACTCAG AGAACAAGAC CTCAAATGCC CGTCCTCTCT GAGCACAGAT 
GTCCCAGAGA CACTACCCAA ACCCATTCCT CGGCCAGCCC CAACCCTTGC AGGAGCGCAT 
GAATTCAGTG AGATAAGAAC GCTGCTGCGT GAATGGGTCA CCACCATCTC AGAACCAATG 
GAGGAGGACA TTTTACAGGT GGTGAAGTAC TGCACAGAAC TAGTGGAGGA CAAAGACCTG 
GAGAAACTCT ACCTTGTCAT CAAGTACATG AAGAGGCTCA TGCAGCAGTC CTCAGAGTCA 
GTGTGGAGCA TGGCCTTTGA CTTTGTCCTG GACAATGTGC AGGTGGTGGT ACAGCAAACC 
TACGGCAGCA CTTTGAAGAT CACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

REV1 ( XM_005167585.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005167642.1 Danio rerio REV1, polymerase (DNA directed) (rev1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa15099
Clone ID Related Accession (Same CDS sequence)	XM_005167585.4 , XM_005167585.3
Accession Version	XM_005167585.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	DNA repair protein REV1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005167585.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCGAG ATGGATGGAG GAAGAAAGCC AGTGAAAATG ATGGGTGGGG AGGACAGGGA 
GGTTACATGG CTGCAAAAGT CTCTAAGCTT GAGGAGCAGT TTCAGAAGGA TGCCCCGAGA 
GAGCAGAAGA AAGATGGAAA GTCCTCCTGC ATCTTCAGCG GTGTGGCCAT CTATGTCAAT 
GGATACACAG ATCCCAGTGC GGCTGAGTTA CGCAGGCTTA TGATGTTGCA TGGTGGGCAA 
TTTCATCTGT ATTACTCTCG CTCCAAAACC ACGCACATCA TTGCCACCAA CCTGCCCAAC 
TTCAAAATAC AAGAGCTCAA AGGAGAGAAA GTAGTGCGGC CAGAGTGGAT CATAGACAGT 
ATAAAAGCAG GTCGCCAACT ATCATATATT CAGTATCAGC TCTATGCGAA GCAGAAAGGA 
TTAAATTTCA CCAGAGTTTC TGGAATAGAA GGCCAGGACC CGTCATCCAA CCATGGACCA 
GACATCAAGC CTCAGCCGAG CCATACTCTA TCTAAAGACG TCCTCAAGTC TACTCAGGCT 
AACCACATGG CTAACTACAC TGAGAAAACC GATCACCAGC TGGATATCAG GCTCAGAAAT 
GGTTTGCATA GCATTATCTC TGAGGTAGAT GCAGAGAAAT CGAGAGTCAA TGGGATTCAT 
GATGATGATG ATGATGATGA CATCGCCTGT TCCATTTTGC CAAGAGGCTC ACAAGACACT 
TTGTTAACTA ACGGTCATGT ACACCCTGTG AATGGTGCCT TAAAGCCTCA GGACTTTGCA 
GACCATCATC CTCATGTTGT TGACAAAGCG TCCAATCAGT CTCACCTCCC ACAGGACAAA 
TACAGGGAGG GTGAGGAGCC CTGCTGCTCA TATACTGAAT CAAAAGCTAA TTCCCATCTG 
AGGTCCTTAA TATCAGCAGC CAGTCCTGCA AAGCAGCCTG GTCCTCACAA TATGCAAACG 
GACCTCCATC AAAAACCAAC ATCCAATTCA GGAACTTTAG GTGCTCCTCA GAAGGCCAGT 
CTGCATGATC AGACCACAGT CCGGCTAAAT GGGAGTTATC ACGTGACCTC AAACTCCTCC 
ACTCTGGCAA ACTCTAGTAA CCAGTCAGGA AAATCCTCTG CAGAGGCTGG AATAATATCC 
GAGTTCTTTT CTCACTCAAG GCTGCATCAC ATTTCTACGT GGCGCAATGA GTTCTCGGAG 
TATGTAAACA GCCTGCAGAG CAGACGACGA GCAGCAGGTG GAGCTGTTTT CTCTGGAAGA 
GAGAAGCTGA AGAAGCTCAA AGCTAACTGC AATTCAGGTT CCTTGATGGC TGCTCCTCAG 
GTGCGTCAGT CATGTGTTCT CCATGTTGAT ATGGACTGCT TCTTTGTCTC TGTGGGGATT 
AGACACCGAC CAGATCTTAT AGGGAAACCA GTTGCGGTGA CTAGTAATCG TGGTCCAGGC 
CGTGTTGCTC AGCGCCCTGG TGCAAACCCT CAGCTTGAGT TTCAGTATTA CCAGAATAAA 
CAGAAACATT ACAGGAAAGA GAAGACAGAT GGGGATCTTG AGATGACCCC ATCTCCACAG 
GATGGTGAAG TTCCCAGTAA TGGAGTGCAT GAAGACCTGG CTGCCCTTTC TATGGCAGAG 
ATTGCCTCTT GTAGTTACGA GGCCAGGCAG GCTGGTGTGC GAAATGGCAT GTTCTTTGGT 
AGGGCCAAGC AGCTGTGTCC AGATCTGCAG TCTGTTCCAT ATGACTTCCA TGCTTATAAA 
GAGGTGGCTC TGGCCATGTA TGAGATCCTG GCCAGTTACA CTCATAACAT TGAGGCGCTG 
AGCTGTGACG AGGCACTGGT TGATGCCACA GCTCTTCTGG TTGAGCTGGG GGTGTCTCCT 
GATGAACTGG CACGGTCCAT CCGTGAAGAC ATAAAGGAGA AGACTGGCTG CTGTGCCTCA 
GTTGGAATGA GTTCCAACAT CCTACTTGCT CGGATGGCAA CCCGTAAGGC TAAACCCAAA 
GGGCAGTATT TACTCAGATC AGAGGAAGTG GATGATTTTA TTAGAGACCA GCCTGTGTCC 
AGTTTACCTG GTGTGGGTCG CTCAATGAGC TCCAAGTTGA CCTCTCTAGG AGTGAGTACC 
TGCGGTGACC TGCAGCAGTT GTCCCTGTCT CAACTGCAGA GGGAGTTTGG GCCACGGACG 
GGACAGACGC TCTTTCGCTT TTGCAGGGGA CTAGATGACA GACCTGTGCG CAGTGAGAAA 
GAGAGAAAGT CTGTGTCTGC GGAGATGAAC TACAACATTC GGTTCACACA GGTTGAGGAG 
GCGGAGTCTT TCCTCAATAA TCTCTCTATG GAGGTACAGA AGAGGTTAGA GGGGGCGGGG 
CTTAGAGGTC GTCGGGTTAC ACTGAAGGTC ATGATGCGGA AACCAGGTGC GCCGGTAGAA 
CCAGCCAAAT ATGGTGGTCA TGGGATATGT GACAACTTTG CCAGGTCTGT TTTACTAGCT 
CAGCCCACAG ACAGTGGTCG AGTGATTGCA TCAGAGGCCA TTAAGCTGTT TCATGCCATG 
AAGCTGAATG TGAAGGACAT GAGGGGAGTG GGGCTGCAGG TGCAGCAGCT GGATGGATCA 
CATGCAGACC CTTCAGGACA AGGGCCCTCT CGTGGCCGAT CAATCAGAGA CCTGCTTCTG 
GCCAAACAGT CTGCCCACAG CCCCAGCAAG GAGTCTCCAT CACAAGATGG TTTAATCAGC 
GCTTCGTCAT CCAGAGCTTT CTCCTCCAAT CAGAAGAGAG TTACCCCTGC ATTATCACCG 
CCACCTTCTA CATCATCATC ATCATCACCA CCACCCTCCT TCACCATTAT AGATCCAATG 
CCTGGAACAA GTAAAGGAGA TTATCACCAT CCACACACCC CCAACCACGT CAGGGCCTGC 
CTAAACATCA GCATCGAGGT GCCATCTCCC TCTCAGGTGG ACCCATCTGT TTTAAAAGCT 
TTACCTATAG ACATAAGGAG ACAAGTAGAG GAAACCTGGA GATACAGAGA GGAGCAGCCC 
AGCACTTCTT CTCATTACTC AACACCACCT CGACCACCCT CCACTCCACC TCCAGGACCT 
TCACTTGTTC TGCAGCTCCC AAACCAGCCT GGTCAACCAT GCACAACGGG CATTATATTA 
GAGCTCCCGG ACTTCTCCCA GGTTGACCCA GATGTGTTTG CAGCACTCCC TAGAGAGCTT 
CAGGAAGAGT TGTGTTCTGC TTACAGAAAT AAGGGAAATG CTCAAGCTCA AGCCAGCACT 
GTAGTGGAAC AAAAAAACTC ATTTCCTCAA CTGAAGCAGC CTGCTGTAGG GAAATTAAAG 
CGCCGCTACA AAAAAAGAAA CACTAGTCCT GCCAAAAATG GTTCAAGTCC ACTAAAAAAA 
ATGTTCCCTG GGAACAGCCC TGCCAAAACC AGCCCCTCCA AAACTCTTCC TCTGCCACTC 
AGAGAACAAG ACCTCAAATG CCCGTCCTCT CTGAGCACAG ATGTCCCAGA GACACTACCC 
AAACCCATTC CTCGGCCAGC CCCAACCCTT GCAGGAGCGC ATGAATTCAG TGAGATAAGA 
ACGCTGCTGC GTGAATGGGT CACCACCATC TCAGAACCAA TGGAGGAGGA CATTTTACAG 
GTGGTGAAGT ACTGCACAGA ACTAGTGGAG GACAAAGACC TGGAGAAACT CTACCTTGTC 
ATCAAGTACA TGAAGAGGCT CATGCAGCAG TCCTCAGAGT CAGTGTGGAG CATGGCCTTT 
GACTTTGTCC TGGACAATGT GCAGGTGGTG GTACAGCAAA CCTACGGCAG CACTTTGAAG 
ATCACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167642.1
CDS	539..4327
Translation	MSRDGWRKKASENDGWGGQGGYMAAKVSKLEEQFQKDAPREQKKDGKSSCIFSGVAIYVNGYTDPSAAELRRLMMLHGGQFHLYYSRSKTTHIIATNLPNFKIQELKGEKVVRPEWIIDSIKAGRQLSYIQYQLYAKQKGLNFTRVSGIEGQDPSSNHGPDIKPQPSHTLSKDVLKSTQANHMANYTEKTDHQLDIRLRNGLHSIISEVDAEKSRVNGIHDDDDDDDIACSILPRGSQDTLLTNGHVHPVNGALKPQDFADHHPHVVDKASNQSHLPQDKYREGEEPCCSYTESKANSHLRSLISAASPAKQPGPHNMQTDLHQKPTSNSGTLGAPQKASLHDQTTVRLNGSYHVTSNSSTLANSSNQSGKSSAEAGIISEFFSHSRLHHISTWRNEFSEYVNSLQSRRRAAGGAVFSGREKLKKLKANCNSGSLMAAPQVRQSCVLHVDMDCFFVSVGIRHRPDLIGKPVAVTSNRGPGRVAQRPGANPQLEFQYYQNKQKHYRKEKTDGDLEMTPSPQDGEVPSNGVHEDLAALSMAEIASCSYEARQAGVRNGMFFGRAKQLCPDLQSVPYDFHAYKEVALAMYEILASYTHNIEALSCDEALVDATALLVELGVSPDELARSIREDIKEKTGCCASVGMSSNILLARMATRKAKPKGQYLLRSEEVDDFIRDQPVSSLPGVGRSMSSKLTSLGVSTCGDLQQLSLSQLQREFGPRTGQTLFRFCRGLDDRPVRSEKERKSVSAEMNYNIRFTQVEEAESFLNNLSMEVQKRLEGAGLRGRRVTLKVMMRKPGAPVEPAKYGGHGICDNFARSVLLAQPTDSGRVIASEAIKLFHAMKLNVKDMRGVGLQVQQLDGSHADPSGQGPSRGRSIRDLLLAKQSAHSPSKESPSQDGLISASSSRAFSSNQKRVTPALSPPPSTSSSSSPPPSFTIIDPMPGTSKGDYHHPHTPNHVRACLNISIEVPSPSQVDPSVLKALPIDIRRQVEETWRYREEQPSTSSHYSTPPRPPSTPPPGPSLVLQLPNQPGQPCTTGIILELPDFSQVDPDVFAALPRELQEELCSAYRNKGNAQAQASTVVEQKNSFPQLKQPAVGKLKRRYKKRNTSPAKNGSSPLKKMFPGNSPAKTSPSKTLPLPLREQDLKCPSSLSTDVPETLPKPIPRPAPTLAGAHEFSEIRTLLREWVTTISEPMEEDILQVVKYCTELVEDKDLEKLYLVIKYMKRLMQQSSESVWSMAFDFVLDNVQVVVQQTYGSTLKIT

Target ORF information:

RefSeq Version	XM_005167585.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio REV1, polymerase (DNA directed) (rev1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167585.4

ORF Insert Sequence:

ATGAGTCGAG ATGGATGGAG GAAGAAAGCC AGTGAAAATG ATGGGTGGGG AGGACAGGGA 
GGTTACATGG CTGCAAAAGT CTCTAAGCTT GAGGAGCAGT TTCAGAAGGA TGCCCCGAGA 
GAGCAGAAGA AAGATGGAAA GTCCTCCTGC ATCTTCAGCG GTGTGGCCAT CTATGTCAAT 
GGATACACAG ATCCCAGTGC GGCTGAGTTA CGCAGGCTTA TGATGTTGCA TGGTGGGCAA 
TTTCATCTGT ATTACTCTCG CTCCAAAACC ACGCACATCA TTGCCACCAA CCTGCCCAAC 
TTCAAAATAC AAGAGCTCAA AGGAGAGAAA GTAGTGCGGC CAGAGTGGAT CATAGACAGT 
ATAAAAGCAG GTCGCCAACT ATCATATATT CAGTATCAGC TCTATGCGAA GCAGAAAGGA 
TTAAATTTCA CCAGAGTTTC TGGAATAGAA GGCCAGGACC CGTCATCCAA CCATGGACCA 
GACATCAAGC CTCAGCCGAG CCATACTCTA TCTAAAGACG TCCTCAAGTC TACTCAGGCT 
AACCACATGG CTAACTACAC TGAGAAAACC GATCACCAGC TGGATATCAG GCTCAGAAAT 
GGTTTGCATA GCATTATCTC TGAGGTAGAT GCAGAGAAAT CGAGAGTCAA TGGGATTCAT 
GATGATGATG ATGATGATGA CATCGCCTGT TCCATTTTGC CAAGAGGCTC ACAAGACACT 
TTGTTAACTA ACGGTCATGT ACACCCTGTG AATGGTGCCT TAAAGCCTCA GGACTTTGCA 
GACCATCATC CTCATGTTGT TGACAAAGCG TCCAATCAGT CTCACCTCCC ACAGGACAAA 
TACAGGGAGG GTGAGGAGCC CTGCTGCTCA TATACTGAAT CAAAAGCTAA TTCCCATCTG 
AGGTCCTTAA TATCAGCAGC CAGTCCTGCA AAGCAGCCTG GTCCTCACAA TATGCAAACG 
GACCTCCATC AAAAACCAAC ATCCAATTCA GGAACTTTAG GTGCTCCTCA GAAGGCCAGT 
CTGCATGATC AGACCACAGT CCGGCTAAAT GGGAGTTATC ACGTGACCTC AAACTCCTCC 
ACTCTGGCAA ACTCTAGTAA CCAGTCAGGA AAATCCTCTG CAGAGGCTGG AATAATATCC 
GAGTTCTTTT CTCACTCAAG GCTGCATCAC ATTTCTACGT GGCGCAATGA GTTCTCGGAG 
TATGTAAACA GCCTGCAGAG CAGACGACGA GCAGCAGGTG GAGCTGTTTT CTCTGGAAGA 
GAGAAGCTGA AGAAGCTCAA AGCTAACTGC AATTCAGGTT CCTTGATGGC TGCTCCTCAG 
GTGCGTCAGT CATGTGTTCT CCATGTTGAT ATGGACTGCT TCTTTGTCTC TGTGGGGATT 
AGACACCGAC CAGATCTTAT AGGGAAACCA GTTGCGGTGA CTAGTAATCG TGGTCCAGGC 
CGTGTTGCTC AGCGCCCTGG TGCAAACCCT CAGCTTGAGT TTCAGTATTA CCAGAATAAA 
CAGAAACATT ACAGGAAAGA GAAGACAGAT GGGGATCTTG AGATGACCCC ATCTCCACAG 
GATGGTGAAG TTCCCAGTAA TGGAGTGCAT GAAGACCTGG CTGCCCTTTC TATGGCAGAG 
ATTGCCTCTT GTAGTTACGA GGCCAGGCAG GCTGGTGTGC GAAATGGCAT GTTCTTTGGT 
AGGGCCAAGC AGCTGTGTCC AGATCTGCAG TCTGTTCCAT ATGACTTCCA TGCTTATAAA 
GAGGTGGCTC TGGCCATGTA TGAGATCCTG GCCAGTTACA CTCATAACAT TGAGGCGCTG 
AGCTGTGACG AGGCACTGGT TGATGCCACA GCTCTTCTGG TTGAGCTGGG GGTGTCTCCT 
GATGAACTGG CACGGTCCAT CCGTGAAGAC ATAAAGGAGA AGACTGGCTG CTGTGCCTCA 
GTTGGAATGA GTTCCAACAT CCTACTTGCT CGGATGGCAA CCCGTAAGGC TAAACCCAAA 
GGGCAGTATT TACTCAGATC AGAGGAAGTG GATGATTTTA TTAGAGACCA GCCTGTGTCC 
AGTTTACCTG GTGTGGGTCG CTCAATGAGC TCCAAGTTGA CCTCTCTAGG AGTGAGTACC 
TGCGGTGACC TGCAGCAGTT GTCCCTGTCT CAACTGCAGA GGGAGTTTGG GCCACGGACG 
GGACAGACGC TCTTTCGCTT TTGCAGGGGA CTAGATGACA GACCTGTGCG CAGTGAGAAA 
GAGAGAAAGT CTGTGTCTGC GGAGATGAAC TACAACATTC GGTTCACACA GGTTGAGGAG 
GCGGAGTCTT TCCTCAATAA TCTCTCTATG GAGGTACAGA AGAGGTTAGA GGGGGCGGGG 
CTTAGAGGTC GTCGGGTTAC ACTGAAGGTC ATGATGCGGA AACCAGGTGC GCCGGTAGAA 
CCAGCCAAAT ATGGTGGTCA TGGGATATGT GACAACTTTG CCAGGTCTGT TTTACTAGCT 
CAGCCCACAG ACAGTGGTCG AGTGATTGCA TCAGAGGCCA TTAAGCTGTT TCATGCCATG 
AAGCTGAATG TGAAGGACAT GAGGGGAGTG GGGCTGCAGG TGCAGCAGCT GGATGGATCA 
CATGCAGACC CTTCAGGACA AGGGCCCTCT CGTGGCCGAT CAATCAGAGA CCTGCTTCTG 
GCCAAACAGT CTGCCCACAG CCCCAGCAAG GAGTCTCCAT CACAAGATGG TTTAATCAGC 
GCTTCGTCAT CCAGAGCTTT CTCCTCCAAT CAGAAGAGAG TTACCCCTGC ATTATCACCG 
CCACCTTCTA CATCATCATC ATCATCACCA CCACCCTCCT TCACCATTAT AGATCCAATG 
CCTGGAACAA GTAAAGGAGA TTATCACCAT CCACACACCC CCAACCACGT CAGGGCCTGC 
CTAAACATCA GCATCGAGGT GCCATCTCCC TCTCAGGTGG ACCCATCTGT TTTAAAAGCT 
TTACCTATAG ACATAAGGAG ACAAGTAGAG GAAACCTGGA GATACAGAGA GGAGCAGCCC 
AGCACTTCTT CTCATTACTC AACACCACCT CGACCACCCT CCACTCCACC TCCAGGACCT 
TCACTTGTTC TGCAGCTCCC AAACCAGCCT GGTCAACCAT GCACAACGGG CATTATATTA 
GAGCTCCCGG ACTTCTCCCA GGTTGACCCA GATGTGTTTG CAGCACTCCC TAGAGAGCTT 
CAGGAAGAGT TGTGTTCTGC TTACAGAAAT AAGGGAAATG CTCAAGCTCA AGCCAGCACT 
GTAGTGGAAC AAAAAAACTC ATTTCCTCAA CTGAAGCAGC CTGCTGTAGG GAAATTAAAG 
CGCCGCTACA AAAAAAGAAA CACTAGTCCT GCCAAAAATG GTTCAAGTCC ACTAAAAAAA 
ATGTTCCCTG GGAACAGCCC TGCCAAAACC AGCCCCTCCA AAACTCTTCC TCTGCCACTC 
AGAGAACAAG ACCTCAAATG CCCGTCCTCT CTGAGCACAG ATGTCCCAGA GACACTACCC 
AAACCCATTC CTCGGCCAGC CCCAACCCTT GCAGGAGCGC ATGAATTCAG TGAGATAAGA 
ACGCTGCTGC GTGAATGGGT CACCACCATC TCAGAACCAA TGGAGGAGGA CATTTTACAG 
GTGGTGAAGT ACTGCACAGA ACTAGTGGAG GACAAAGACC TGGAGAAACT CTACCTTGTC 
ATCAAGTACA TGAAGAGGCT CATGCAGCAG TCCTCAGAGT CAGTGTGGAG CATGGCCTTT 
GACTTTGTCC TGGACAATGT GCAGGTGGTG GTACAGCAAA CCTACGGCAG CACTTTGAAG 
ATCACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

REV1 ( XM_005167585.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005167642.1 Danio rerio REV1, polymerase (DNA directed) (rev1), transcript variant X1, mRNA.

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CloneID	ODa15099
Clone ID Related Accession (Same CDS sequence)	XM_005167585.4 , XM_005167585.3
Accession Version	XM_005167585.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	DNA repair protein REV1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167585.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCGAG ATGGATGGAG GAAGAAAGCC AGTGAAAATG ATGGGTGGGG AGGACAGGGA 
GGTTACATGG CTGCAAAAGT CTCTAAGCTT GAGGAGCAGT TTCAGAAGGA TGCCCCGAGA 
GAGCAGAAGA AAGATGGAAA GTCCTCCTGC ATCTTCAGCG GTGTGGCCAT CTATGTCAAT 
GGATACACAG ATCCCAGTGC GGCTGAGTTA CGCAGGCTTA TGATGTTGCA TGGTGGGCAA 
TTTCATCTGT ATTACTCTCG CTCCAAAACC ACGCACATCA TTGCCACCAA CCTGCCCAAC 
TTCAAAATAC AAGAGCTCAA AGGAGAGAAA GTAGTGCGGC CAGAGTGGAT CATAGACAGT 
ATAAAAGCAG GTCGCCAACT ATCATATATT CAGTATCAGC TCTATGCGAA GCAGAAAGGA 
TTAAATTTCA CCAGAGTTTC TGGAATAGAA GGCCAGGACC CGTCATCCAA CCATGGACCA 
GACATCAAGC CTCAGCCGAG CCATACTCTA TCTAAAGACG TCCTCAAGTC TACTCAGGCT 
AACCACATGG CTAACTACAC TGAGAAAACC GATCACCAGC TGGATATCAG GCTCAGAAAT 
GGTTTGCATA GCATTATCTC TGAGGTAGAT GCAGAGAAAT CGAGAGTCAA TGGGATTCAT 
GATGATGATG ATGATGATGA CATCGCCTGT TCCATTTTGC CAAGAGGCTC ACAAGACACT 
TTGTTAACTA ACGGTCATGT ACACCCTGTG AATGGTGCCT TAAAGCCTCA GGACTTTGCA 
GACCATCATC CTCATGTTGT TGACAAAGCG TCCAATCAGT CTCACCTCCC ACAGGACAAA 
TACAGGGAGG GTGAGGAGCC CTGCTGCTCA TATACTGAAT CAAAAGCTAA TTCCCATCTG 
AGGTCCTTAA TATCAGCAGC CAGTCCTGCA AAGCAGCCTG GTCCTCACAA TATGCAAACG 
GACCTCCATC AAAAACCAAC ATCCAATTCA GGAACTTTAG GTGCTCCTCA GAAGGCCAGT 
CTGCATGATC AGACCACAGT CCGGCTAAAT GGGAGTTATC ACGTGACCTC AAACTCCTCC 
ACTCTGGCAA ACTCTAGTAA CCAGTCAGGA AAATCCTCTG CAGAGGCTGG AATAATATCC 
GAGTTCTTTT CTCACTCAAG GCTGCATCAC ATTTCTACGT GGCGCAATGA GTTCTCGGAG 
TATGTAAACA GCCTGCAGAG CAGACGACGA GCAGCAGGTG GAGCTGTTTT CTCTGGAAGA 
GAGAAGCTGA AGAAGCTCAA AGCTAACTGC AATTCAGGTT CCTTGATGGC TGCTCCTCAG 
GTGCGTCAGT CATGTGTTCT CCATGTTGAT ATGGACTGCT TCTTTGTCTC TGTGGGGATT 
AGACACCGAC CAGATCTTAT AGGGAAACCA GTTGCGGTGA CTAGTAATCG TGGTCCAGGC 
CGTGTTGCTC AGCGCCCTGG TGCAAACCCT CAGCTTGAGT TTCAGTATTA CCAGAATAAA 
CAGAAACATT ACAGGAAAGA GAAGACAGAT GGGGATCTTG AGATGACCCC ATCTCCACAG 
GATGGTGAAG TTCCCAGTAA TGGAGTGCAT GAAGACCTGG CTGCCCTTTC TATGGCAGAG 
ATTGCCTCTT GTAGTTACGA GGCCAGGCAG GCTGGTGTGC GAAATGGCAT GTTCTTTGGT 
AGGGCCAAGC AGCTGTGTCC AGATCTGCAG TCTGTTCCAT ATGACTTCCA TGCTTATAAA 
GAGGTGGCTC TGGCCATGTA TGAGATCCTG GCCAGTTACA CTCATAACAT TGAGGCGCTG 
AGCTGTGACG AGGCACTGGT TGATGCCACA GCTCTTCTGG TTGAGCTGGG GGTGTCTCCT 
GATGAACTGG CACGGTCCAT CCGTGAAGAC ATAAAGGAGA AGACTGGCTG CTGTGCCTCA 
GTTGGAATGA GTTCCAACAT CCTACTTGCT CGGATGGCAA CCCGTAAGGC TAAACCCAAA 
GGGCAGTATT TACTCAGATC AGAGGAAGTG GATGATTTTA TTAGAGACCA GCCTGTGTCC 
AGTTTACCTG GTGTGGGTCG CTCAATGAGC TCCAAGTTGA CCTCTCTAGG AGTGAGTACC 
TGCGGTGACC TGCAGCAGTT GTCCCTGTCT CAACTGCAGA GGGAGTTTGG GCCACGGACG 
GGACAGACGC TCTTTCGCTT TTGCAGGGGA CTAGATGACA GACCTGTGCG CAGTGAGAAA 
GAGAGAAAGT CTGTGTCTGC GGAGATGAAC TACAACATTC GGTTCACACA GGTTGAGGAG 
GCGGAGTCTT TCCTCAATAA TCTCTCTATG GAGGTACAGA AGAGGTTAGA GGGGGCGGGG 
CTTAGAGGTC GTCGGGTTAC ACTGAAGGTC ATGATGCGGA AACCAGGTGC GCCGGTAGAA 
CCAGCCAAAT ATGGTGGTCA TGGGATATGT GACAACTTTG CCAGGTCTGT TTTACTAGCT 
CAGCCCACAG ACAGTGGTCG AGTGATTGCA TCAGAGGCCA TTAAGCTGTT TCATGCCATG 
AAGCTGAATG TGAAGGACAT GAGGGGAGTG GGGCTGCAGG TGCAGCAGCT GGATGGATCA 
CATGCAGACC CTTCAGGACA AGGGCCCTCT CGTGGCCGAT CAATCAGAGA CCTGCTTCTG 
GCCAAACAGT CTGCCCACAG CCCCAGCAAG GAGTCTCCAT CACAAGATGG TTTAATCAGC 
GCTTCGTCAT CCAGAGCTTT CTCCTCCAAT CAGAAGAGAG TTACCCCTGC ATTATCACCG 
CCACCTTCTA CATCATCATC ATCATCACCA CCACCCTCCT TCACCATTAT AGATCCAATG 
CCTGGAACAA GTAAAGGAGA TTATCACCAT CCACACACCC CCAACCACGT CAGGGCCTGC 
CTAAACATCA GCATCGAGGT GCCATCTCCC TCTCAGGTGG ACCCATCTGT TTTAAAAGCT 
TTACCTATAG ACATAAGGAG ACAAGTAGAG GAAACCTGGA GATACAGAGA GGAGCAGCCC 
AGCACTTCTT CTCATTACTC AACACCACCT CGACCACCCT CCACTCCACC TCCAGGACCT 
TCACTTGTTC TGCAGCTCCC AAACCAGCCT GGTCAACCAT GCACAACGGG CATTATATTA 
GAGCTCCCGG ACTTCTCCCA GGTTGACCCA GATGTGTTTG CAGCACTCCC TAGAGAGCTT 
CAGGAAGAGT TGTGTTCTGC TTACAGAAAT AAGGGAAATG CTCAAGCTCA AGCCAGCACT 
GTAGTGGAAC AAAAAAACTC ATTTCCTCAA CTGAAGCAGC CTGCTGTAGG GAAATTAAAG 
CGCCGCTACA AAAAAAGAAA CACTAGTCCT GCCAAAAATG GTTCAAGTCC ACTAAAAAAA 
ATGTTCCCTG GGAACAGCCC TGCCAAAACC AGCCCCTCCA AAACTCTTCC TCTGCCACTC 
AGAGAACAAG ACCTCAAATG CCCGTCCTCT CTGAGCACAG ATGTCCCAGA GACACTACCC 
AAACCCATTC CTCGGCCAGC CCCAACCCTT GCAGGAGCGC ATGAATTCAG TGAGATAAGA 
ACGCTGCTGC GTGAATGGGT CACCACCATC TCAGAACCAA TGGAGGAGGA CATTTTACAG 
GTGGTGAAGT ACTGCACAGA ACTAGTGGAG GACAAAGACC TGGAGAAACT CTACCTTGTC 
ATCAAGTACA TGAAGAGGCT CATGCAGCAG TCCTCAGAGT CAGTGTGGAG CATGGCCTTT 
GACTTTGTCC TGGACAATGT GCAGGTGGTG GTACAGCAAA CCTACGGCAG CACTTTGAAG 
ATCACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167642.1
CDS	421..4209
Translation	MSRDGWRKKASENDGWGGQGGYMAAKVSKLEEQFQKDAPREQKKDGKSSCIFSGVAIYVNGYTDPSAAELRRLMMLHGGQFHLYYSRSKTTHIIATNLPNFKIQELKGEKVVRPEWIIDSIKAGRQLSYIQYQLYAKQKGLNFTRVSGIEGQDPSSNHGPDIKPQPSHTLSKDVLKSTQANHMANYTEKTDHQLDIRLRNGLHSIISEVDAEKSRVNGIHDDDDDDDIACSILPRGSQDTLLTNGHVHPVNGALKPQDFADHHPHVVDKASNQSHLPQDKYREGEEPCCSYTESKANSHLRSLISAASPAKQPGPHNMQTDLHQKPTSNSGTLGAPQKASLHDQTTVRLNGSYHVTSNSSTLANSSNQSGKSSAEAGIISEFFSHSRLHHISTWRNEFSEYVNSLQSRRRAAGGAVFSGREKLKKLKANCNSGSLMAAPQVRQSCVLHVDMDCFFVSVGIRHRPDLIGKPVAVTSNRGPGRVAQRPGANPQLEFQYYQNKQKHYRKEKTDGDLEMTPSPQDGEVPSNGVHEDLAALSMAEIASCSYEARQAGVRNGMFFGRAKQLCPDLQSVPYDFHAYKEVALAMYEILASYTHNIEALSCDEALVDATALLVELGVSPDELARSIREDIKEKTGCCASVGMSSNILLARMATRKAKPKGQYLLRSEEVDDFIRDQPVSSLPGVGRSMSSKLTSLGVSTCGDLQQLSLSQLQREFGPRTGQTLFRFCRGLDDRPVRSEKERKSVSAEMNYNIRFTQVEEAESFLNNLSMEVQKRLEGAGLRGRRVTLKVMMRKPGAPVEPAKYGGHGICDNFARSVLLAQPTDSGRVIASEAIKLFHAMKLNVKDMRGVGLQVQQLDGSHADPSGQGPSRGRSIRDLLLAKQSAHSPSKESPSQDGLISASSSRAFSSNQKRVTPALSPPPSTSSSSSPPPSFTIIDPMPGTSKGDYHHPHTPNHVRACLNISIEVPSPSQVDPSVLKALPIDIRRQVEETWRYREEQPSTSSHYSTPPRPPSTPPPGPSLVLQLPNQPGQPCTTGIILELPDFSQVDPDVFAALPRELQEELCSAYRNKGNAQAQASTVVEQKNSFPQLKQPAVGKLKRRYKKRNTSPAKNGSSPLKKMFPGNSPAKTSPSKTLPLPLREQDLKCPSSLSTDVPETLPKPIPRPAPTLAGAHEFSEIRTLLREWVTTISEPMEEDILQVVKYCTELVEDKDLEKLYLVIKYMKRLMQQSSESVWSMAFDFVLDNVQVVVQQTYGSTLKIT

Target ORF information:

RefSeq Version	XM_005167585.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio REV1, polymerase (DNA directed) (rev1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167585.3

ORF Insert Sequence:

ATGAGTCGAG ATGGATGGAG GAAGAAAGCC AGTGAAAATG ATGGGTGGGG AGGACAGGGA 
GGTTACATGG CTGCAAAAGT CTCTAAGCTT GAGGAGCAGT TTCAGAAGGA TGCCCCGAGA 
GAGCAGAAGA AAGATGGAAA GTCCTCCTGC ATCTTCAGCG GTGTGGCCAT CTATGTCAAT 
GGATACACAG ATCCCAGTGC GGCTGAGTTA CGCAGGCTTA TGATGTTGCA TGGTGGGCAA 
TTTCATCTGT ATTACTCTCG CTCCAAAACC ACGCACATCA TTGCCACCAA CCTGCCCAAC 
TTCAAAATAC AAGAGCTCAA AGGAGAGAAA GTAGTGCGGC CAGAGTGGAT CATAGACAGT 
ATAAAAGCAG GTCGCCAACT ATCATATATT CAGTATCAGC TCTATGCGAA GCAGAAAGGA 
TTAAATTTCA CCAGAGTTTC TGGAATAGAA GGCCAGGACC CGTCATCCAA CCATGGACCA 
GACATCAAGC CTCAGCCGAG CCATACTCTA TCTAAAGACG TCCTCAAGTC TACTCAGGCT 
AACCACATGG CTAACTACAC TGAGAAAACC GATCACCAGC TGGATATCAG GCTCAGAAAT 
GGTTTGCATA GCATTATCTC TGAGGTAGAT GCAGAGAAAT CGAGAGTCAA TGGGATTCAT 
GATGATGATG ATGATGATGA CATCGCCTGT TCCATTTTGC CAAGAGGCTC ACAAGACACT 
TTGTTAACTA ACGGTCATGT ACACCCTGTG AATGGTGCCT TAAAGCCTCA GGACTTTGCA 
GACCATCATC CTCATGTTGT TGACAAAGCG TCCAATCAGT CTCACCTCCC ACAGGACAAA 
TACAGGGAGG GTGAGGAGCC CTGCTGCTCA TATACTGAAT CAAAAGCTAA TTCCCATCTG 
AGGTCCTTAA TATCAGCAGC CAGTCCTGCA AAGCAGCCTG GTCCTCACAA TATGCAAACG 
GACCTCCATC AAAAACCAAC ATCCAATTCA GGAACTTTAG GTGCTCCTCA GAAGGCCAGT 
CTGCATGATC AGACCACAGT CCGGCTAAAT GGGAGTTATC ACGTGACCTC AAACTCCTCC 
ACTCTGGCAA ACTCTAGTAA CCAGTCAGGA AAATCCTCTG CAGAGGCTGG AATAATATCC 
GAGTTCTTTT CTCACTCAAG GCTGCATCAC ATTTCTACGT GGCGCAATGA GTTCTCGGAG 
TATGTAAACA GCCTGCAGAG CAGACGACGA GCAGCAGGTG GAGCTGTTTT CTCTGGAAGA 
GAGAAGCTGA AGAAGCTCAA AGCTAACTGC AATTCAGGTT CCTTGATGGC TGCTCCTCAG 
GTGCGTCAGT CATGTGTTCT CCATGTTGAT ATGGACTGCT TCTTTGTCTC TGTGGGGATT 
AGACACCGAC CAGATCTTAT AGGGAAACCA GTTGCGGTGA CTAGTAATCG TGGTCCAGGC 
CGTGTTGCTC AGCGCCCTGG TGCAAACCCT CAGCTTGAGT TTCAGTATTA CCAGAATAAA 
CAGAAACATT ACAGGAAAGA GAAGACAGAT GGGGATCTTG AGATGACCCC ATCTCCACAG 
GATGGTGAAG TTCCCAGTAA TGGAGTGCAT GAAGACCTGG CTGCCCTTTC TATGGCAGAG 
ATTGCCTCTT GTAGTTACGA GGCCAGGCAG GCTGGTGTGC GAAATGGCAT GTTCTTTGGT 
AGGGCCAAGC AGCTGTGTCC AGATCTGCAG TCTGTTCCAT ATGACTTCCA TGCTTATAAA 
GAGGTGGCTC TGGCCATGTA TGAGATCCTG GCCAGTTACA CTCATAACAT TGAGGCGCTG 
AGCTGTGACG AGGCACTGGT TGATGCCACA GCTCTTCTGG TTGAGCTGGG GGTGTCTCCT 
GATGAACTGG CACGGTCCAT CCGTGAAGAC ATAAAGGAGA AGACTGGCTG CTGTGCCTCA 
GTTGGAATGA GTTCCAACAT CCTACTTGCT CGGATGGCAA CCCGTAAGGC TAAACCCAAA 
GGGCAGTATT TACTCAGATC AGAGGAAGTG GATGATTTTA TTAGAGACCA GCCTGTGTCC 
AGTTTACCTG GTGTGGGTCG CTCAATGAGC TCCAAGTTGA CCTCTCTAGG AGTGAGTACC 
TGCGGTGACC TGCAGCAGTT GTCCCTGTCT CAACTGCAGA GGGAGTTTGG GCCACGGACG 
GGACAGACGC TCTTTCGCTT TTGCAGGGGA CTAGATGACA GACCTGTGCG CAGTGAGAAA 
GAGAGAAAGT CTGTGTCTGC GGAGATGAAC TACAACATTC GGTTCACACA GGTTGAGGAG 
GCGGAGTCTT TCCTCAATAA TCTCTCTATG GAGGTACAGA AGAGGTTAGA GGGGGCGGGG 
CTTAGAGGTC GTCGGGTTAC ACTGAAGGTC ATGATGCGGA AACCAGGTGC GCCGGTAGAA 
CCAGCCAAAT ATGGTGGTCA TGGGATATGT GACAACTTTG CCAGGTCTGT TTTACTAGCT 
CAGCCCACAG ACAGTGGTCG AGTGATTGCA TCAGAGGCCA TTAAGCTGTT TCATGCCATG 
AAGCTGAATG TGAAGGACAT GAGGGGAGTG GGGCTGCAGG TGCAGCAGCT GGATGGATCA 
CATGCAGACC CTTCAGGACA AGGGCCCTCT CGTGGCCGAT CAATCAGAGA CCTGCTTCTG 
GCCAAACAGT CTGCCCACAG CCCCAGCAAG GAGTCTCCAT CACAAGATGG TTTAATCAGC 
GCTTCGTCAT CCAGAGCTTT CTCCTCCAAT CAGAAGAGAG TTACCCCTGC ATTATCACCG 
CCACCTTCTA CATCATCATC ATCATCACCA CCACCCTCCT TCACCATTAT AGATCCAATG 
CCTGGAACAA GTAAAGGAGA TTATCACCAT CCACACACCC CCAACCACGT CAGGGCCTGC 
CTAAACATCA GCATCGAGGT GCCATCTCCC TCTCAGGTGG ACCCATCTGT TTTAAAAGCT 
TTACCTATAG ACATAAGGAG ACAAGTAGAG GAAACCTGGA GATACAGAGA GGAGCAGCCC 
AGCACTTCTT CTCATTACTC AACACCACCT CGACCACCCT CCACTCCACC TCCAGGACCT 
TCACTTGTTC TGCAGCTCCC AAACCAGCCT GGTCAACCAT GCACAACGGG CATTATATTA 
GAGCTCCCGG ACTTCTCCCA GGTTGACCCA GATGTGTTTG CAGCACTCCC TAGAGAGCTT 
CAGGAAGAGT TGTGTTCTGC TTACAGAAAT AAGGGAAATG CTCAAGCTCA AGCCAGCACT 
GTAGTGGAAC AAAAAAACTC ATTTCCTCAA CTGAAGCAGC CTGCTGTAGG GAAATTAAAG 
CGCCGCTACA AAAAAAGAAA CACTAGTCCT GCCAAAAATG GTTCAAGTCC ACTAAAAAAA 
ATGTTCCCTG GGAACAGCCC TGCCAAAACC AGCCCCTCCA AAACTCTTCC TCTGCCACTC 
AGAGAACAAG ACCTCAAATG CCCGTCCTCT CTGAGCACAG ATGTCCCAGA GACACTACCC 
AAACCCATTC CTCGGCCAGC CCCAACCCTT GCAGGAGCGC ATGAATTCAG TGAGATAAGA 
ACGCTGCTGC GTGAATGGGT CACCACCATC TCAGAACCAA TGGAGGAGGA CATTTTACAG 
GTGGTGAAGT ACTGCACAGA ACTAGTGGAG GACAAAGACC TGGAGAAACT CTACCTTGTC 
ATCAAGTACA TGAAGAGGCT CATGCAGCAG TCCTCAGAGT CAGTGTGGAG CATGGCCTTT 
GACTTTGTCC TGGACAATGT GCAGGTGGTG GTACAGCAAA CCTACGGCAG CACTTTGAAG 
ATCACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

REV1 ( XM_009304637.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009302912.1 Danio rerio REV1, polymerase (DNA directed) (rev1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa45890
Clone ID Related Accession (Same CDS sequence)	XM_009304637.2 , XM_009304637.3
Accession Version	XM_009304637.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	DNA repair protein REV1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304637.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCGAG ATGGATGGAG GAAGAAAGCC AGTGAAAATG ATGGGTGGGG AGGACAGGGA 
GGTTACATGG CTGCAAAAGT CTCTAAGCTT GAGGAGCAGT TTCAGAAGGA TGCCCCGAGA 
GAGCAGAAGA AAGATGGAAA GTCCTCCTGC ATCTTCAGCG GTGTGGCCAT CTATGTCAAT 
GGATACACAG ATCCCAGTGC GGCTGAGTTA CGCAGGCTTA TGATGTTGCA TGGTGGGCAA 
TTTCATCTGT ATTACTCTCG CTCCAAAACC ACGCACATCA TTGCCACCAA CCTGCCCAAC 
TTCAAAATAC AAGAGCTCAA AGGAGAGAAA GTAGTGCGGC CAGAGTGGAT CATAGACAGT 
ATAAAAGCAG GTCGCCAACT ATCATATATT CAGTATCAGC TCTATGCGAA GCAGAAAGGA 
TTAAATTTCA CCAGAGTTTC TGGAATAGAA GGCCAGGACC CGTCATCCAA CCATGGACCA 
GACATCAAGC CTCAGCCGAG CCATACTCTA TCTAAAGACG TCCTCAAGTC TACTCAGGCT 
AACCACATGG CTAACTACAC TGAGAAAACC GATCACCAGC TGGATATCAG GCTAAATGGT 
TTGCATAGCA TTATCTCTGA GGTAGATGCA GAGAAATCGA GAGTCAATGG GATTCATGAT 
GATGATGATG ATGATGACAT CGCCTGTTCC ATTTTGCCAA GAGGCTCACA AGACACTTTG 
TTAACTAACG GTCATGTACA CCCTGTGAAT GGTGCCTTAA AGCCTCAGGA CTTTGCAGAC 
CATCATCCTC ATGTTGTTGA CAAAGCGTCC AATCAGTCTC ACCTCCCACA GGACAAATAC 
AGGGAGGGTG AGGAGCCCTG CTGCTCATAT ACTGAATCAA AAGCTAATTC CCATCTGAGG 
TCCTTAATAT CAGCAGCCAG TCCTGCAAAG CAGCCTGGTC CTCACAATAT GCAAACGGAC 
CTCCATCAAA AACCAACATC CAATTCAGGA ACTTTAGGTG CTCCTCAGAA GGCCAGTCTG 
CATGATCAGA CCACAGTCCG GCTAAATGGG AGTTATCACG TGACCTCAAA CTCCTCCACT 
CTGGCAAACT CTAGTAACCA GTCAGGAAAA TCCTCTGCAG AGGCTGGAAT AATATCCGAG 
TTCTTTTCTC ACTCAAGGCT GCATCACATT TCTACGTGGC GCAATGAGTT CTCGGAGTAT 
GTAAACAGCC TGCAGAGCAG ACGACGAGCA GCAGGTGGAG CTGTTTTCTC TGGAAGAGAG 
AAGCTGAAGA AGCTCAAAGC TAACTGCAAT TCAGGTTCCT TGATGGCTGC TCCTCAGGTG 
CGTCAGTCAT GTGTTCTCCA TGTTGATATG GACTGCTTCT TTGTCTCTGT GGGGATTAGA 
CACCGACCAG ATCTTATAGG GAAACCAGTT GCGGTGACTA GTAATCGTGG TCCAGGCCGT 
GTTGCTCAGC GCCCTGGTGC AAACCCTCAG CTTGAGTTTC AGTATTACCA GAATAAACAG 
AAACATTACA GGAAAGAGAA GACAGATGGG GATCTTGAGA TGACCCCATC TCCACAGGAT 
GGTGAAGTTC CCAGTAATGG AGTGCATGAA GACCTGGCTG CCCTTTCTAT GGCAGAGATT 
GCCTCTTGTA GTTACGAGGC CAGGCAGGCT GGTGTGCGAA ATGGCATGTT CTTTGGTAGG 
GCCAAGCAGC TGTGTCCAGA TCTGCAGTCT GTTCCATATG ACTTCCATGC TTATAAAGAG 
GTGGCTCTGG CCATGTATGA GATCCTGGCC AGTTACACTC ATAACATTGA GGCGCTGAGC 
TGTGACGAGG CACTGGTTGA TGCCACAGCT CTTCTGGTTG AGCTGGGGGT GTCTCCTGAT 
GAACTGGCAC GGTCCATCCG TGAAGACATA AAGGAGAAGA CTGGCTGCTG TGCCTCAGTT 
GGAATGAGTT CCAACATCCT ACTTGCTCGG ATGGCAACCC GTAAGGCTAA ACCCAAAGGG 
CAGTATTTAC TCAGATCAGA GGAAGTGGAT GATTTTATTA GAGACCAGCC TGTGTCCAGT 
TTACCTGGTG TGGGTCGCTC AATGAGCTCC AAGTTGACCT CTCTAGGAGT GAGTACCTGC 
GGTGACCTGC AGCAGTTGTC CCTGTCTCAA CTGCAGAGGG AGTTTGGGCC ACGGACGGGA 
CAGACGCTCT TTCGCTTTTG CAGGGGACTA GATGACAGAC CTGTGCGCAG TGAGAAAGAG 
AGAAAGTCTG TGTCTGCGGA GATGAACTAC AACATTCGGT TCACACAGGT TGAGGAGGCG 
GAGTCTTTCC TCAATAATCT CTCTATGGAG GTACAGAAGA GGTTAGAGGG GGCGGGGCTT 
AGAGGTCGTC GGGTTACACT GAAGGTCATG ATGCGGAAAC CAGGTGCGCC GGTAGAACCA 
GCCAAATATG GTGGTCATGG GATATGTGAC AACTTTGCCA GGTCTGTTTT ACTAGCTCAG 
CCCACAGACA GTGGTCGAGT GATTGCATCA GAGGCCATTA AGCTGTTTCA TGCCATGAAG 
CTGAATGTGA AGGACATGAG GGGAGTGGGG CTGCAGGTGC AGCAGCTGGA TGGATCACAT 
GCAGACCCTT CAGGACAAGG GCCCTCTCGT GGCCGATCAA TCAGAGACCT GCTTCTGGCC 
AAACAGTCTG CCCACAGCCC CAGCAAGGAG TCTCCATCAC AAGATGGTTT AATCAGCGCT 
TCGTCATCCA GAGCTTTCTC CTCCAATCAG AAGAGAGTTA CCCCTGCATT ATCACCGCCA 
CCTTCTACAT CATCATCATC ATCACCACCA CCCTCCTTCA CCATTATAGA TCCAATGCCT 
GGAACAAGTA AAGGAGATTA TCACCATCCA CACACCCCCA ACCACGTCAG GGCCTGCCTA 
AACATCAGCA TCGAGGTGCC ATCTCCCTCT CAGGTGGACC CATCTGTTTT AAAAGCTTTA 
CCTATAGACA TAAGGAGACA AGTAGAGGAA ACCTGGAGAT ACAGAGAGGA GCAGCCCAGC 
ACTTCTTCTC ATTACTCAAC ACCACCTCGA CCACCCTCCA CTCCACCTCC AGGACCTTCA 
CTTGTTCTGC AGCTCCCAAA CCAGCCTGGT CAACCATGCA CAACGGGCAT TATATTAGAG 
CTCCCGGACT TCTCCCAGGT TGACCCAGAT GTGTTTGCAG CACTCCCTAG AGAGCTTCAG 
GAAGAGTTGT GTTCTGCTTA CAGAAATAAG GGAAATGCTC AAGCTCAAGC CAGCACTGTA 
GTGGAACAAA AAAACTCATT TCCTCAACTG AAGCAGCCTG CTGTAGGGAA ATTAAAGCGC 
CGCTACAAAA AAAGAAACAC TAGTCCTGCC AAAAATGGTT CAAGTCCACT AAAAAAAATG 
TTCCCTGGGA ACAGCCCTGC CAAAACCAGC CCCTCCAAAA CTCTTCCTCT GCCACTCAGA 
GAACAAGACC TCAAATGCCC GTCCTCTCTG AGCACAGATG TCCCAGAGAC ACTACCCAAA 
CCCATTCCTC GGCCAGCCCC AACCCTTGCA GGAGCGCATG AATTCAGTGA GATAAGAACG 
CTGCTGCGTG AATGGGTCAC CACCATCTCA GAACCAATGG AGGAGGACAT TTTACAGGTG 
GTGAAGTACT GCACAGAACT AGTGGAGGAC AAAGACCTGG AGAAACTCTA CCTTGTCATC 
AAGTACATGA AGAGGCTCAT GCAGCAGTCC TCAGAGTCAG TGTGGAGCAT GGCCTTTGAC 
TTTGTCCTGG ACAATGTGCA GGTGGTGGTA CAGCAAACCT ACGGCAGCAC TTTGAAGATC 
ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302912.1
CDS	420..4205
Translation	MSRDGWRKKASENDGWGGQGGYMAAKVSKLEEQFQKDAPREQKKDGKSSCIFSGVAIYVNGYTDPSAAELRRLMMLHGGQFHLYYSRSKTTHIIATNLPNFKIQELKGEKVVRPEWIIDSIKAGRQLSYIQYQLYAKQKGLNFTRVSGIEGQDPSSNHGPDIKPQPSHTLSKDVLKSTQANHMANYTEKTDHQLDIRLNGLHSIISEVDAEKSRVNGIHDDDDDDDIACSILPRGSQDTLLTNGHVHPVNGALKPQDFADHHPHVVDKASNQSHLPQDKYREGEEPCCSYTESKANSHLRSLISAASPAKQPGPHNMQTDLHQKPTSNSGTLGAPQKASLHDQTTVRLNGSYHVTSNSSTLANSSNQSGKSSAEAGIISEFFSHSRLHHISTWRNEFSEYVNSLQSRRRAAGGAVFSGREKLKKLKANCNSGSLMAAPQVRQSCVLHVDMDCFFVSVGIRHRPDLIGKPVAVTSNRGPGRVAQRPGANPQLEFQYYQNKQKHYRKEKTDGDLEMTPSPQDGEVPSNGVHEDLAALSMAEIASCSYEARQAGVRNGMFFGRAKQLCPDLQSVPYDFHAYKEVALAMYEILASYTHNIEALSCDEALVDATALLVELGVSPDELARSIREDIKEKTGCCASVGMSSNILLARMATRKAKPKGQYLLRSEEVDDFIRDQPVSSLPGVGRSMSSKLTSLGVSTCGDLQQLSLSQLQREFGPRTGQTLFRFCRGLDDRPVRSEKERKSVSAEMNYNIRFTQVEEAESFLNNLSMEVQKRLEGAGLRGRRVTLKVMMRKPGAPVEPAKYGGHGICDNFARSVLLAQPTDSGRVIASEAIKLFHAMKLNVKDMRGVGLQVQQLDGSHADPSGQGPSRGRSIRDLLLAKQSAHSPSKESPSQDGLISASSSRAFSSNQKRVTPALSPPPSTSSSSSPPPSFTIIDPMPGTSKGDYHHPHTPNHVRACLNISIEVPSPSQVDPSVLKALPIDIRRQVEETWRYREEQPSTSSHYSTPPRPPSTPPPGPSLVLQLPNQPGQPCTTGIILELPDFSQVDPDVFAALPRELQEELCSAYRNKGNAQAQASTVVEQKNSFPQLKQPAVGKLKRRYKKRNTSPAKNGSSPLKKMFPGNSPAKTSPSKTLPLPLREQDLKCPSSLSTDVPETLPKPIPRPAPTLAGAHEFSEIRTLLREWVTTISEPMEEDILQVVKYCTELVEDKDLEKLYLVIKYMKRLMQQSSESVWSMAFDFVLDNVQVVVQQTYGSTLKIT

Target ORF information:

RefSeq Version	XM_009304637.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio REV1, polymerase (DNA directed) (rev1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304637.2

ORF Insert Sequence:

ATGAGTCGAG ATGGATGGAG GAAGAAAGCC AGTGAAAATG ATGGGTGGGG AGGACAGGGA 
GGTTACATGG CTGCAAAAGT CTCTAAGCTT GAGGAGCAGT TTCAGAAGGA TGCCCCGAGA 
GAGCAGAAGA AAGATGGAAA GTCCTCCTGC ATCTTCAGCG GTGTGGCCAT CTATGTCAAT 
GGATACACAG ATCCCAGTGC GGCTGAGTTA CGCAGGCTTA TGATGTTGCA TGGTGGGCAA 
TTTCATCTGT ATTACTCTCG CTCCAAAACC ACGCACATCA TTGCCACCAA CCTGCCCAAC 
TTCAAAATAC AAGAGCTCAA AGGAGAGAAA GTAGTGCGGC CAGAGTGGAT CATAGACAGT 
ATAAAAGCAG GTCGCCAACT ATCATATATT CAGTATCAGC TCTATGCGAA GCAGAAAGGA 
TTAAATTTCA CCAGAGTTTC TGGAATAGAA GGCCAGGACC CGTCATCCAA CCATGGACCA 
GACATCAAGC CTCAGCCGAG CCATACTCTA TCTAAAGACG TCCTCAAGTC TACTCAGGCT 
AACCACATGG CTAACTACAC TGAGAAAACC GATCACCAGC TGGATATCAG GCTAAATGGT 
TTGCATAGCA TTATCTCTGA GGTAGATGCA GAGAAATCGA GAGTCAATGG GATTCATGAT 
GATGATGATG ATGATGACAT CGCCTGTTCC ATTTTGCCAA GAGGCTCACA AGACACTTTG 
TTAACTAACG GTCATGTACA CCCTGTGAAT GGTGCCTTAA AGCCTCAGGA CTTTGCAGAC 
CATCATCCTC ATGTTGTTGA CAAAGCGTCC AATCAGTCTC ACCTCCCACA GGACAAATAC 
AGGGAGGGTG AGGAGCCCTG CTGCTCATAT ACTGAATCAA AAGCTAATTC CCATCTGAGG 
TCCTTAATAT CAGCAGCCAG TCCTGCAAAG CAGCCTGGTC CTCACAATAT GCAAACGGAC 
CTCCATCAAA AACCAACATC CAATTCAGGA ACTTTAGGTG CTCCTCAGAA GGCCAGTCTG 
CATGATCAGA CCACAGTCCG GCTAAATGGG AGTTATCACG TGACCTCAAA CTCCTCCACT 
CTGGCAAACT CTAGTAACCA GTCAGGAAAA TCCTCTGCAG AGGCTGGAAT AATATCCGAG 
TTCTTTTCTC ACTCAAGGCT GCATCACATT TCTACGTGGC GCAATGAGTT CTCGGAGTAT 
GTAAACAGCC TGCAGAGCAG ACGACGAGCA GCAGGTGGAG CTGTTTTCTC TGGAAGAGAG 
AAGCTGAAGA AGCTCAAAGC TAACTGCAAT TCAGGTTCCT TGATGGCTGC TCCTCAGGTG 
CGTCAGTCAT GTGTTCTCCA TGTTGATATG GACTGCTTCT TTGTCTCTGT GGGGATTAGA 
CACCGACCAG ATCTTATAGG GAAACCAGTT GCGGTGACTA GTAATCGTGG TCCAGGCCGT 
GTTGCTCAGC GCCCTGGTGC AAACCCTCAG CTTGAGTTTC AGTATTACCA GAATAAACAG 
AAACATTACA GGAAAGAGAA GACAGATGGG GATCTTGAGA TGACCCCATC TCCACAGGAT 
GGTGAAGTTC CCAGTAATGG AGTGCATGAA GACCTGGCTG CCCTTTCTAT GGCAGAGATT 
GCCTCTTGTA GTTACGAGGC CAGGCAGGCT GGTGTGCGAA ATGGCATGTT CTTTGGTAGG 
GCCAAGCAGC TGTGTCCAGA TCTGCAGTCT GTTCCATATG ACTTCCATGC TTATAAAGAG 
GTGGCTCTGG CCATGTATGA GATCCTGGCC AGTTACACTC ATAACATTGA GGCGCTGAGC 
TGTGACGAGG CACTGGTTGA TGCCACAGCT CTTCTGGTTG AGCTGGGGGT GTCTCCTGAT 
GAACTGGCAC GGTCCATCCG TGAAGACATA AAGGAGAAGA CTGGCTGCTG TGCCTCAGTT 
GGAATGAGTT CCAACATCCT ACTTGCTCGG ATGGCAACCC GTAAGGCTAA ACCCAAAGGG 
CAGTATTTAC TCAGATCAGA GGAAGTGGAT GATTTTATTA GAGACCAGCC TGTGTCCAGT 
TTACCTGGTG TGGGTCGCTC AATGAGCTCC AAGTTGACCT CTCTAGGAGT GAGTACCTGC 
GGTGACCTGC AGCAGTTGTC CCTGTCTCAA CTGCAGAGGG AGTTTGGGCC ACGGACGGGA 
CAGACGCTCT TTCGCTTTTG CAGGGGACTA GATGACAGAC CTGTGCGCAG TGAGAAAGAG 
AGAAAGTCTG TGTCTGCGGA GATGAACTAC AACATTCGGT TCACACAGGT TGAGGAGGCG 
GAGTCTTTCC TCAATAATCT CTCTATGGAG GTACAGAAGA GGTTAGAGGG GGCGGGGCTT 
AGAGGTCGTC GGGTTACACT GAAGGTCATG ATGCGGAAAC CAGGTGCGCC GGTAGAACCA 
GCCAAATATG GTGGTCATGG GATATGTGAC AACTTTGCCA GGTCTGTTTT ACTAGCTCAG 
CCCACAGACA GTGGTCGAGT GATTGCATCA GAGGCCATTA AGCTGTTTCA TGCCATGAAG 
CTGAATGTGA AGGACATGAG GGGAGTGGGG CTGCAGGTGC AGCAGCTGGA TGGATCACAT 
GCAGACCCTT CAGGACAAGG GCCCTCTCGT GGCCGATCAA TCAGAGACCT GCTTCTGGCC 
AAACAGTCTG CCCACAGCCC CAGCAAGGAG TCTCCATCAC AAGATGGTTT AATCAGCGCT 
TCGTCATCCA GAGCTTTCTC CTCCAATCAG AAGAGAGTTA CCCCTGCATT ATCACCGCCA 
CCTTCTACAT CATCATCATC ATCACCACCA CCCTCCTTCA CCATTATAGA TCCAATGCCT 
GGAACAAGTA AAGGAGATTA TCACCATCCA CACACCCCCA ACCACGTCAG GGCCTGCCTA 
AACATCAGCA TCGAGGTGCC ATCTCCCTCT CAGGTGGACC CATCTGTTTT AAAAGCTTTA 
CCTATAGACA TAAGGAGACA AGTAGAGGAA ACCTGGAGAT ACAGAGAGGA GCAGCCCAGC 
ACTTCTTCTC ATTACTCAAC ACCACCTCGA CCACCCTCCA CTCCACCTCC AGGACCTTCA 
CTTGTTCTGC AGCTCCCAAA CCAGCCTGGT CAACCATGCA CAACGGGCAT TATATTAGAG 
CTCCCGGACT TCTCCCAGGT TGACCCAGAT GTGTTTGCAG CACTCCCTAG AGAGCTTCAG 
GAAGAGTTGT GTTCTGCTTA CAGAAATAAG GGAAATGCTC AAGCTCAAGC CAGCACTGTA 
GTGGAACAAA AAAACTCATT TCCTCAACTG AAGCAGCCTG CTGTAGGGAA ATTAAAGCGC 
CGCTACAAAA AAAGAAACAC TAGTCCTGCC AAAAATGGTT CAAGTCCACT AAAAAAAATG 
TTCCCTGGGA ACAGCCCTGC CAAAACCAGC CCCTCCAAAA CTCTTCCTCT GCCACTCAGA 
GAACAAGACC TCAAATGCCC GTCCTCTCTG AGCACAGATG TCCCAGAGAC ACTACCCAAA 
CCCATTCCTC GGCCAGCCCC AACCCTTGCA GGAGCGCATG AATTCAGTGA GATAAGAACG 
CTGCTGCGTG AATGGGTCAC CACCATCTCA GAACCAATGG AGGAGGACAT TTTACAGGTG 
GTGAAGTACT GCACAGAACT AGTGGAGGAC AAAGACCTGG AGAAACTCTA CCTTGTCATC 
AAGTACATGA AGAGGCTCAT GCAGCAGTCC TCAGAGTCAG TGTGGAGCAT GGCCTTTGAC 
TTTGTCCTGG ACAATGTGCA GGTGGTGGTA CAGCAAACCT ACGGCAGCAC TTTGAAGATC 
ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

REV1 ( XM_009304637.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009302912.1 Danio rerio REV1, polymerase (DNA directed) (rev1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa45890
Clone ID Related Accession (Same CDS sequence)	XM_009304637.2 , XM_009304637.3
Accession Version	XM_009304637.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	DNA repair protein REV1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304637.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCGAG ATGGATGGAG GAAGAAAGCC AGTGAAAATG ATGGGTGGGG AGGACAGGGA 
GGTTACATGG CTGCAAAAGT CTCTAAGCTT GAGGAGCAGT TTCAGAAGGA TGCCCCGAGA 
GAGCAGAAGA AAGATGGAAA GTCCTCCTGC ATCTTCAGCG GTGTGGCCAT CTATGTCAAT 
GGATACACAG ATCCCAGTGC GGCTGAGTTA CGCAGGCTTA TGATGTTGCA TGGTGGGCAA 
TTTCATCTGT ATTACTCTCG CTCCAAAACC ACGCACATCA TTGCCACCAA CCTGCCCAAC 
TTCAAAATAC AAGAGCTCAA AGGAGAGAAA GTAGTGCGGC CAGAGTGGAT CATAGACAGT 
ATAAAAGCAG GTCGCCAACT ATCATATATT CAGTATCAGC TCTATGCGAA GCAGAAAGGA 
TTAAATTTCA CCAGAGTTTC TGGAATAGAA GGCCAGGACC CGTCATCCAA CCATGGACCA 
GACATCAAGC CTCAGCCGAG CCATACTCTA TCTAAAGACG TCCTCAAGTC TACTCAGGCT 
AACCACATGG CTAACTACAC TGAGAAAACC GATCACCAGC TGGATATCAG GCTAAATGGT 
TTGCATAGCA TTATCTCTGA GGTAGATGCA GAGAAATCGA GAGTCAATGG GATTCATGAT 
GATGATGATG ATGATGACAT CGCCTGTTCC ATTTTGCCAA GAGGCTCACA AGACACTTTG 
TTAACTAACG GTCATGTACA CCCTGTGAAT GGTGCCTTAA AGCCTCAGGA CTTTGCAGAC 
CATCATCCTC ATGTTGTTGA CAAAGCGTCC AATCAGTCTC ACCTCCCACA GGACAAATAC 
AGGGAGGGTG AGGAGCCCTG CTGCTCATAT ACTGAATCAA AAGCTAATTC CCATCTGAGG 
TCCTTAATAT CAGCAGCCAG TCCTGCAAAG CAGCCTGGTC CTCACAATAT GCAAACGGAC 
CTCCATCAAA AACCAACATC CAATTCAGGA ACTTTAGGTG CTCCTCAGAA GGCCAGTCTG 
CATGATCAGA CCACAGTCCG GCTAAATGGG AGTTATCACG TGACCTCAAA CTCCTCCACT 
CTGGCAAACT CTAGTAACCA GTCAGGAAAA TCCTCTGCAG AGGCTGGAAT AATATCCGAG 
TTCTTTTCTC ACTCAAGGCT GCATCACATT TCTACGTGGC GCAATGAGTT CTCGGAGTAT 
GTAAACAGCC TGCAGAGCAG ACGACGAGCA GCAGGTGGAG CTGTTTTCTC TGGAAGAGAG 
AAGCTGAAGA AGCTCAAAGC TAACTGCAAT TCAGGTTCCT TGATGGCTGC TCCTCAGGTG 
CGTCAGTCAT GTGTTCTCCA TGTTGATATG GACTGCTTCT TTGTCTCTGT GGGGATTAGA 
CACCGACCAG ATCTTATAGG GAAACCAGTT GCGGTGACTA GTAATCGTGG TCCAGGCCGT 
GTTGCTCAGC GCCCTGGTGC AAACCCTCAG CTTGAGTTTC AGTATTACCA GAATAAACAG 
AAACATTACA GGAAAGAGAA GACAGATGGG GATCTTGAGA TGACCCCATC TCCACAGGAT 
GGTGAAGTTC CCAGTAATGG AGTGCATGAA GACCTGGCTG CCCTTTCTAT GGCAGAGATT 
GCCTCTTGTA GTTACGAGGC CAGGCAGGCT GGTGTGCGAA ATGGCATGTT CTTTGGTAGG 
GCCAAGCAGC TGTGTCCAGA TCTGCAGTCT GTTCCATATG ACTTCCATGC TTATAAAGAG 
GTGGCTCTGG CCATGTATGA GATCCTGGCC AGTTACACTC ATAACATTGA GGCGCTGAGC 
TGTGACGAGG CACTGGTTGA TGCCACAGCT CTTCTGGTTG AGCTGGGGGT GTCTCCTGAT 
GAACTGGCAC GGTCCATCCG TGAAGACATA AAGGAGAAGA CTGGCTGCTG TGCCTCAGTT 
GGAATGAGTT CCAACATCCT ACTTGCTCGG ATGGCAACCC GTAAGGCTAA ACCCAAAGGG 
CAGTATTTAC TCAGATCAGA GGAAGTGGAT GATTTTATTA GAGACCAGCC TGTGTCCAGT 
TTACCTGGTG TGGGTCGCTC AATGAGCTCC AAGTTGACCT CTCTAGGAGT GAGTACCTGC 
GGTGACCTGC AGCAGTTGTC CCTGTCTCAA CTGCAGAGGG AGTTTGGGCC ACGGACGGGA 
CAGACGCTCT TTCGCTTTTG CAGGGGACTA GATGACAGAC CTGTGCGCAG TGAGAAAGAG 
AGAAAGTCTG TGTCTGCGGA GATGAACTAC AACATTCGGT TCACACAGGT TGAGGAGGCG 
GAGTCTTTCC TCAATAATCT CTCTATGGAG GTACAGAAGA GGTTAGAGGG GGCGGGGCTT 
AGAGGTCGTC GGGTTACACT GAAGGTCATG ATGCGGAAAC CAGGTGCGCC GGTAGAACCA 
GCCAAATATG GTGGTCATGG GATATGTGAC AACTTTGCCA GGTCTGTTTT ACTAGCTCAG 
CCCACAGACA GTGGTCGAGT GATTGCATCA GAGGCCATTA AGCTGTTTCA TGCCATGAAG 
CTGAATGTGA AGGACATGAG GGGAGTGGGG CTGCAGGTGC AGCAGCTGGA TGGATCACAT 
GCAGACCCTT CAGGACAAGG GCCCTCTCGT GGCCGATCAA TCAGAGACCT GCTTCTGGCC 
AAACAGTCTG CCCACAGCCC CAGCAAGGAG TCTCCATCAC AAGATGGTTT AATCAGCGCT 
TCGTCATCCA GAGCTTTCTC CTCCAATCAG AAGAGAGTTA CCCCTGCATT ATCACCGCCA 
CCTTCTACAT CATCATCATC ATCACCACCA CCCTCCTTCA CCATTATAGA TCCAATGCCT 
GGAACAAGTA AAGGAGATTA TCACCATCCA CACACCCCCA ACCACGTCAG GGCCTGCCTA 
AACATCAGCA TCGAGGTGCC ATCTCCCTCT CAGGTGGACC CATCTGTTTT AAAAGCTTTA 
CCTATAGACA TAAGGAGACA AGTAGAGGAA ACCTGGAGAT ACAGAGAGGA GCAGCCCAGC 
ACTTCTTCTC ATTACTCAAC ACCACCTCGA CCACCCTCCA CTCCACCTCC AGGACCTTCA 
CTTGTTCTGC AGCTCCCAAA CCAGCCTGGT CAACCATGCA CAACGGGCAT TATATTAGAG 
CTCCCGGACT TCTCCCAGGT TGACCCAGAT GTGTTTGCAG CACTCCCTAG AGAGCTTCAG 
GAAGAGTTGT GTTCTGCTTA CAGAAATAAG GGAAATGCTC AAGCTCAAGC CAGCACTGTA 
GTGGAACAAA AAAACTCATT TCCTCAACTG AAGCAGCCTG CTGTAGGGAA ATTAAAGCGC 
CGCTACAAAA AAAGAAACAC TAGTCCTGCC AAAAATGGTT CAAGTCCACT AAAAAAAATG 
TTCCCTGGGA ACAGCCCTGC CAAAACCAGC CCCTCCAAAA CTCTTCCTCT GCCACTCAGA 
GAACAAGACC TCAAATGCCC GTCCTCTCTG AGCACAGATG TCCCAGAGAC ACTACCCAAA 
CCCATTCCTC GGCCAGCCCC AACCCTTGCA GGAGCGCATG AATTCAGTGA GATAAGAACG 
CTGCTGCGTG AATGGGTCAC CACCATCTCA GAACCAATGG AGGAGGACAT TTTACAGGTG 
GTGAAGTACT GCACAGAACT AGTGGAGGAC AAAGACCTGG AGAAACTCTA CCTTGTCATC 
AAGTACATGA AGAGGCTCAT GCAGCAGTCC TCAGAGTCAG TGTGGAGCAT GGCCTTTGAC 
TTTGTCCTGG ACAATGTGCA GGTGGTGGTA CAGCAAACCT ACGGCAGCAC TTTGAAGATC 
ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302912.1
CDS	538..4323
Translation	MSRDGWRKKASENDGWGGQGGYMAAKVSKLEEQFQKDAPREQKKDGKSSCIFSGVAIYVNGYTDPSAAELRRLMMLHGGQFHLYYSRSKTTHIIATNLPNFKIQELKGEKVVRPEWIIDSIKAGRQLSYIQYQLYAKQKGLNFTRVSGIEGQDPSSNHGPDIKPQPSHTLSKDVLKSTQANHMANYTEKTDHQLDIRLNGLHSIISEVDAEKSRVNGIHDDDDDDDIACSILPRGSQDTLLTNGHVHPVNGALKPQDFADHHPHVVDKASNQSHLPQDKYREGEEPCCSYTESKANSHLRSLISAASPAKQPGPHNMQTDLHQKPTSNSGTLGAPQKASLHDQTTVRLNGSYHVTSNSSTLANSSNQSGKSSAEAGIISEFFSHSRLHHISTWRNEFSEYVNSLQSRRRAAGGAVFSGREKLKKLKANCNSGSLMAAPQVRQSCVLHVDMDCFFVSVGIRHRPDLIGKPVAVTSNRGPGRVAQRPGANPQLEFQYYQNKQKHYRKEKTDGDLEMTPSPQDGEVPSNGVHEDLAALSMAEIASCSYEARQAGVRNGMFFGRAKQLCPDLQSVPYDFHAYKEVALAMYEILASYTHNIEALSCDEALVDATALLVELGVSPDELARSIREDIKEKTGCCASVGMSSNILLARMATRKAKPKGQYLLRSEEVDDFIRDQPVSSLPGVGRSMSSKLTSLGVSTCGDLQQLSLSQLQREFGPRTGQTLFRFCRGLDDRPVRSEKERKSVSAEMNYNIRFTQVEEAESFLNNLSMEVQKRLEGAGLRGRRVTLKVMMRKPGAPVEPAKYGGHGICDNFARSVLLAQPTDSGRVIASEAIKLFHAMKLNVKDMRGVGLQVQQLDGSHADPSGQGPSRGRSIRDLLLAKQSAHSPSKESPSQDGLISASSSRAFSSNQKRVTPALSPPPSTSSSSSPPPSFTIIDPMPGTSKGDYHHPHTPNHVRACLNISIEVPSPSQVDPSVLKALPIDIRRQVEETWRYREEQPSTSSHYSTPPRPPSTPPPGPSLVLQLPNQPGQPCTTGIILELPDFSQVDPDVFAALPRELQEELCSAYRNKGNAQAQASTVVEQKNSFPQLKQPAVGKLKRRYKKRNTSPAKNGSSPLKKMFPGNSPAKTSPSKTLPLPLREQDLKCPSSLSTDVPETLPKPIPRPAPTLAGAHEFSEIRTLLREWVTTISEPMEEDILQVVKYCTELVEDKDLEKLYLVIKYMKRLMQQSSESVWSMAFDFVLDNVQVVVQQTYGSTLKIT

Target ORF information:

RefSeq Version	XM_009304637.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio REV1, polymerase (DNA directed) (rev1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304637.3

ORF Insert Sequence:

ATGAGTCGAG ATGGATGGAG GAAGAAAGCC AGTGAAAATG ATGGGTGGGG AGGACAGGGA 
GGTTACATGG CTGCAAAAGT CTCTAAGCTT GAGGAGCAGT TTCAGAAGGA TGCCCCGAGA 
GAGCAGAAGA AAGATGGAAA GTCCTCCTGC ATCTTCAGCG GTGTGGCCAT CTATGTCAAT 
GGATACACAG ATCCCAGTGC GGCTGAGTTA CGCAGGCTTA TGATGTTGCA TGGTGGGCAA 
TTTCATCTGT ATTACTCTCG CTCCAAAACC ACGCACATCA TTGCCACCAA CCTGCCCAAC 
TTCAAAATAC AAGAGCTCAA AGGAGAGAAA GTAGTGCGGC CAGAGTGGAT CATAGACAGT 
ATAAAAGCAG GTCGCCAACT ATCATATATT CAGTATCAGC TCTATGCGAA GCAGAAAGGA 
TTAAATTTCA CCAGAGTTTC TGGAATAGAA GGCCAGGACC CGTCATCCAA CCATGGACCA 
GACATCAAGC CTCAGCCGAG CCATACTCTA TCTAAAGACG TCCTCAAGTC TACTCAGGCT 
AACCACATGG CTAACTACAC TGAGAAAACC GATCACCAGC TGGATATCAG GCTAAATGGT 
TTGCATAGCA TTATCTCTGA GGTAGATGCA GAGAAATCGA GAGTCAATGG GATTCATGAT 
GATGATGATG ATGATGACAT CGCCTGTTCC ATTTTGCCAA GAGGCTCACA AGACACTTTG 
TTAACTAACG GTCATGTACA CCCTGTGAAT GGTGCCTTAA AGCCTCAGGA CTTTGCAGAC 
CATCATCCTC ATGTTGTTGA CAAAGCGTCC AATCAGTCTC ACCTCCCACA GGACAAATAC 
AGGGAGGGTG AGGAGCCCTG CTGCTCATAT ACTGAATCAA AAGCTAATTC CCATCTGAGG 
TCCTTAATAT CAGCAGCCAG TCCTGCAAAG CAGCCTGGTC CTCACAATAT GCAAACGGAC 
CTCCATCAAA AACCAACATC CAATTCAGGA ACTTTAGGTG CTCCTCAGAA GGCCAGTCTG 
CATGATCAGA CCACAGTCCG GCTAAATGGG AGTTATCACG TGACCTCAAA CTCCTCCACT 
CTGGCAAACT CTAGTAACCA GTCAGGAAAA TCCTCTGCAG AGGCTGGAAT AATATCCGAG 
TTCTTTTCTC ACTCAAGGCT GCATCACATT TCTACGTGGC GCAATGAGTT CTCGGAGTAT 
GTAAACAGCC TGCAGAGCAG ACGACGAGCA GCAGGTGGAG CTGTTTTCTC TGGAAGAGAG 
AAGCTGAAGA AGCTCAAAGC TAACTGCAAT TCAGGTTCCT TGATGGCTGC TCCTCAGGTG 
CGTCAGTCAT GTGTTCTCCA TGTTGATATG GACTGCTTCT TTGTCTCTGT GGGGATTAGA 
CACCGACCAG ATCTTATAGG GAAACCAGTT GCGGTGACTA GTAATCGTGG TCCAGGCCGT 
GTTGCTCAGC GCCCTGGTGC AAACCCTCAG CTTGAGTTTC AGTATTACCA GAATAAACAG 
AAACATTACA GGAAAGAGAA GACAGATGGG GATCTTGAGA TGACCCCATC TCCACAGGAT 
GGTGAAGTTC CCAGTAATGG AGTGCATGAA GACCTGGCTG CCCTTTCTAT GGCAGAGATT 
GCCTCTTGTA GTTACGAGGC CAGGCAGGCT GGTGTGCGAA ATGGCATGTT CTTTGGTAGG 
GCCAAGCAGC TGTGTCCAGA TCTGCAGTCT GTTCCATATG ACTTCCATGC TTATAAAGAG 
GTGGCTCTGG CCATGTATGA GATCCTGGCC AGTTACACTC ATAACATTGA GGCGCTGAGC 
TGTGACGAGG CACTGGTTGA TGCCACAGCT CTTCTGGTTG AGCTGGGGGT GTCTCCTGAT 
GAACTGGCAC GGTCCATCCG TGAAGACATA AAGGAGAAGA CTGGCTGCTG TGCCTCAGTT 
GGAATGAGTT CCAACATCCT ACTTGCTCGG ATGGCAACCC GTAAGGCTAA ACCCAAAGGG 
CAGTATTTAC TCAGATCAGA GGAAGTGGAT GATTTTATTA GAGACCAGCC TGTGTCCAGT 
TTACCTGGTG TGGGTCGCTC AATGAGCTCC AAGTTGACCT CTCTAGGAGT GAGTACCTGC 
GGTGACCTGC AGCAGTTGTC CCTGTCTCAA CTGCAGAGGG AGTTTGGGCC ACGGACGGGA 
CAGACGCTCT TTCGCTTTTG CAGGGGACTA GATGACAGAC CTGTGCGCAG TGAGAAAGAG 
AGAAAGTCTG TGTCTGCGGA GATGAACTAC AACATTCGGT TCACACAGGT TGAGGAGGCG 
GAGTCTTTCC TCAATAATCT CTCTATGGAG GTACAGAAGA GGTTAGAGGG GGCGGGGCTT 
AGAGGTCGTC GGGTTACACT GAAGGTCATG ATGCGGAAAC CAGGTGCGCC GGTAGAACCA 
GCCAAATATG GTGGTCATGG GATATGTGAC AACTTTGCCA GGTCTGTTTT ACTAGCTCAG 
CCCACAGACA GTGGTCGAGT GATTGCATCA GAGGCCATTA AGCTGTTTCA TGCCATGAAG 
CTGAATGTGA AGGACATGAG GGGAGTGGGG CTGCAGGTGC AGCAGCTGGA TGGATCACAT 
GCAGACCCTT CAGGACAAGG GCCCTCTCGT GGCCGATCAA TCAGAGACCT GCTTCTGGCC 
AAACAGTCTG CCCACAGCCC CAGCAAGGAG TCTCCATCAC AAGATGGTTT AATCAGCGCT 
TCGTCATCCA GAGCTTTCTC CTCCAATCAG AAGAGAGTTA CCCCTGCATT ATCACCGCCA 
CCTTCTACAT CATCATCATC ATCACCACCA CCCTCCTTCA CCATTATAGA TCCAATGCCT 
GGAACAAGTA AAGGAGATTA TCACCATCCA CACACCCCCA ACCACGTCAG GGCCTGCCTA 
AACATCAGCA TCGAGGTGCC ATCTCCCTCT CAGGTGGACC CATCTGTTTT AAAAGCTTTA 
CCTATAGACA TAAGGAGACA AGTAGAGGAA ACCTGGAGAT ACAGAGAGGA GCAGCCCAGC 
ACTTCTTCTC ATTACTCAAC ACCACCTCGA CCACCCTCCA CTCCACCTCC AGGACCTTCA 
CTTGTTCTGC AGCTCCCAAA CCAGCCTGGT CAACCATGCA CAACGGGCAT TATATTAGAG 
CTCCCGGACT TCTCCCAGGT TGACCCAGAT GTGTTTGCAG CACTCCCTAG AGAGCTTCAG 
GAAGAGTTGT GTTCTGCTTA CAGAAATAAG GGAAATGCTC AAGCTCAAGC CAGCACTGTA 
GTGGAACAAA AAAACTCATT TCCTCAACTG AAGCAGCCTG CTGTAGGGAA ATTAAAGCGC 
CGCTACAAAA AAAGAAACAC TAGTCCTGCC AAAAATGGTT CAAGTCCACT AAAAAAAATG 
TTCCCTGGGA ACAGCCCTGC CAAAACCAGC CCCTCCAAAA CTCTTCCTCT GCCACTCAGA 
GAACAAGACC TCAAATGCCC GTCCTCTCTG AGCACAGATG TCCCAGAGAC ACTACCCAAA 
CCCATTCCTC GGCCAGCCCC AACCCTTGCA GGAGCGCATG AATTCAGTGA GATAAGAACG 
CTGCTGCGTG AATGGGTCAC CACCATCTCA GAACCAATGG AGGAGGACAT TTTACAGGTG 
GTGAAGTACT GCACAGAACT AGTGGAGGAC AAAGACCTGG AGAAACTCTA CCTTGTCATC 
AAGTACATGA AGAGGCTCAT GCAGCAGTCC TCAGAGTCAG TGTGGAGCAT GGCCTTTGAC 
TTTGTCCTGG ACAATGTGCA GGTGGTGGTA CAGCAAACCT ACGGCAGCAC TTTGAAGATC 
ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

REV1 ( XM_017357713.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017213202.1 Danio rerio REV1, polymerase (DNA directed) (rev1), transcript variant X3, mRNA.

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CloneID	ODa60270
Clone ID Related Accession (Same CDS sequence)	XM_017357713.1
Accession Version	XM_017357713.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	DNA repair protein REV1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCGAG ATGGATGGAG GAAGAAAGCC AGTGAAAATG ATGGGTGGGG AGGACAGGGA 
GGTTACATGG CTGCAAAAGT CTCTAAGCTT GAGGAGCAGT TTCAGAAGGA TGCCCCGAGA 
GAGCAGAAGA AAGATGGAAA GTCCTCCTGC ATCTTCAGCG GTGTGGCCAT CTATGTCAAT 
GGATACACAG ATCCCAGTGC GGCTGAGTTA CGCAGGCTTA TGATGTTGCA TGGTGGGCAA 
TTTCATCTGT ATTACTCTCG CTCCAAAACC ACGCACATCA TTGCCACCAA CCTGCCCAAC 
TTCAAAATAC AAGAGCTCAA AGGAGAGAAA GTAGTGCGGC CAGAGTGGAT CATAGACAGT 
ATAAAAGCAG GTCGCCAACT ATCATATATT CAGTATCAGC TCTATGCGAA GCAGAAAGGA 
TTAAATTTCA CCAGAGTTTC TGGAATAGAA GGCCAGGACC CGTCATCCAA CCATGGACCA 
GACATCAAGC CTCAGCCGAG CCATACTCTA TCTAAAGACG TCCTCAAGTC TACTCAGGCT 
AACCACATGG CTAACTACAC TGAGAAAACC GATCACCAGC TGGATATCAG GCTCAGAAAT 
GGTTTGCATA GCATTATCTC TGAGGTAGAT GCAGAGAAAT CGAGAGTCAA TGGGATTCAT 
GATGATGATG ATGATGATGA CATCGCCTGT TCCATTTTGC CAAGAGGCTC ACAAGACACT 
TTGTTAACTA ACGGTCATGT ACACCCTGTG AATGGTGCCT TAAAGCCTCA GGACTTTGCA 
GACCATCATC CTCATGTTGT TGACAAAGCG TCCAATCAGT CTCACCTCCC ACAGGACAAA 
TACAGGGAGG GTGAGGAGCC CTGCTGCTCA TATACTGAAT CAAAAGCTAA TTCCCATCTG 
AGGTCCTTAA TATCAGCAGC CAGTCCTGCA AAGCAGCCTG GTCCTCACAA TATGCAAACG 
GACCTCCATC AAAAACCAAC ATCCAATTCA GGAACTTTAG GTGCTCCTCA GAAGGCCAGT 
CTGCATGATC AGACCACAGT CCGGCTAAAT GGGAGTTATC ACGTGACCTC AAACTCCTCC 
ACTCTGGCAA ACTCTAGTAA CCAGTCAGGA AAATCCTCTG CAGAGGCTGG AATAATATCC 
GAGTTCTTTT CTCACTCAAG GCTGCATCAC ATTTCTACGT GGCGCAATGA GTTCTCGGAG 
TATGTAAACA GCCTGCAGAG CAGACGACGA GCAGCAGGTG GAGCTGTTTT CTCTGGAAGA 
GAGAAGCTGA AGAAGCTCAA AGCTAACTGC AATTCAGGTT CCTTGATGGC TGCTCCTCAG 
GTGCGTCAGT CATGTGTTCT CCATGTTGAT ATGGACTGCT TCTTTGTCTC TGTGGGGATT 
AGACACCGAC CAGATCTTAT AGGGAAACCA GTTGCGGTGA CTAGTAATCG TGGTCCAGGC 
CGTGTTGCTC AGCGCCCTGG TGCAAACCCT CAGCTTGAGT TTCAGTATTA CCAGAATAAA 
CAGAAACATT ACAGGAAAGA GAAGACAGAT GGGGATCTTG AGATGACCCC ATCTCCACAG 
GATGGTGAAG TTCCCAGTAA TGGAGTGCAT GAAGACCTGG CTGCCCTTTC TATGGCAGAG 
ATTGCCTCTT GTAGTTACGA GGCCAGGCAG GCTGGTGTGC GAAATGGCAT GTTCTTTGGT 
AGGGCCAAGC AGCTGTGTCC AGATCTGCAG TCTGTTCCAT ATGACTTCCA TGCTTATAAA 
GAGGTGGCTC TGGCCATGTA TGAGATCCTG GCCAGTTACA CTCATAACAT TGAGGCGCTG 
AGCTGTGACG AGGCACTGGT TGATGCCACA GCTCTTCTGG TTGAGCTGGG GGTGTCTCCT 
GATGAACTGG CACGGTCCAT CCGTGAAGAC ATAAAGGAGA AGACTGGCTG CTGTGCCTCA 
GTTGGAATGA GTTCCAACAT CCTACTTGCT CGGATGGCAA CCCGTAAGGC TAAACCCAAA 
GGGCAGTATT TACTCAGATC AGAGGAAGTG GATGATTTTA TTAGAGACCA GCCTGTGTCC 
AGTTTACCTG GTGTGGGTCG CTCAATGAGC TCCAAGTTGA CCTCTCTAGG AGTGAGTACC 
TGCGGTGACC TGCAGCAGTT GTCCCTGTCT CAACTGCAGA GGGAGTTTGG GCCACGGACG 
GGACAGACGC TCTTTCGCTT TTGCAGGGGA CTAGATGACA GACCTGTGCG CAGTGAGAAA 
GAGAGAAAGT CTGTGTCTGC GGAGATGAAC TACAACATTC GGTTCACACA GGTTGAGGAG 
GCGGAGTCTT TCCTCAATAA TCTCTCTATG GAGGTACAGA AGAGGTTAGA GGGGGCGGGG 
CTTAGAGGTC GTCGGGTTAC ACTGAAGGTC ATGATGCGGA AACCAGGTGC GCCGGTAGAA 
CCAGCCAAAT ATGGTGGTCA TGGGATATGT GACAACTTTG CCAGGTCTGT TTTACTAGCT 
CAGCCCACAG ACAGTGGTCG AGTGATTGCA TCAGAGGCCA TTAAGCTGTT TCATGCCATG 
AAGCTGAATG TGAAGGACAT GAGGGGAGTG GGGCTGCAGG TGCAGCAGCT GGATGGATCA 
CATGCAGACC CTTCAGGACA AGGGCCCTCT CGTGGCCGAT CAATCAGAGA CCTGCTTCTG 
GCCAAACAGT CTGCCCACAG CCCCAGCAAG GAGTCTCCAT CACAAGATGG TTTAATCAGC 
GCTTCGTCAT CCAGAGCTTT CTCCTCCAAT CAGAAGAGAG TTACCCCTGC ATTATCACCG 
CCACCTTCTA CATCATCATC ATCATCACCA CCACCCTCCT TCACCATTAT AGATCCAATG 
CCTGGAACAA GTAAAGGAGA TTATCACCAT CCACACACCC CCAACCACGT CAGGGCCTGC 
CTAAACATCA GCATCGAGGT GCCATCTCCC TCTCAGGTGG ACCCATCTGT TTTAAAAGCT 
TTACCTATAG ACATAAGGAG ACAAGTAGAG GAAACCTGGA GATACAGAGA GGAGCAGCCC 
AGCACTTCTT CTCATTACTC AACACCACCT CGACCACCCT CCACTCCACC TCCAGGACCT 
TCACTTGTTC TGCAGCTCCC AAACCAGCCT GGTCAACCAT GCACAACGGG CATTATATTA 
GAGCTCCCGG ACTTCTCCCA GGTTGACCCA GATGTGTTTG CAGCACTCCC TAGAGAGCTT 
CAGGAAGAGT TGTGTTCTGC TTACAGAAAT AAGGGAAATG CTCAAGCTCA AGCCAGCACT 
GTGGAACAAA AAAACTCATT TCCTCAACTG AAGCAGCCTG CTGTAGGGAA ATTAAAGCGC 
CGCTACAAAA AAAGAAACAC TAGTCCTGCC AAAAATGGTT CAAGTCCACT AAAAAAAATG 
TTCCCTGGGA ACAGCCCTGC CAAAACCAGC CCCTCCAAAA CTCTTCCTCT GCCACTCAGA 
GAACAAGACC TCAAATGCCC GTCCTCTCTG AGCACAGATG TCCCAGAGAC ACTACCCAAA 
CCCATTCCTC GGCCAGCCCC AACCCTTGCA GGAGCGCATG AATTCAGTGA GATAAGAACG 
CTGCTGCGTG AATGGGTCAC CACCATCTCA GAACCAATGG AGGAGGACAT TTTACAGGTG 
GTGAAGTACT GCACAGAACT AGTGGAGGAC AAAGACCTGG AGAAACTCTA CCTTGTCATC 
AAGTACATGA AGAGGCTCAT GCAGCAGTCC TCAGAGTCAG TGTGGAGCAT GGCCTTTGAC 
TTTGTCCTGG ACAATGTGCA GGTGGTGGTA CAGCAAACCT ACGGCAGCAC TTTGAAGATC 
ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213202.1
CDS	425..4210
Translation	MSRDGWRKKASENDGWGGQGGYMAAKVSKLEEQFQKDAPREQKKDGKSSCIFSGVAIYVNGYTDPSAAELRRLMMLHGGQFHLYYSRSKTTHIIATNLPNFKIQELKGEKVVRPEWIIDSIKAGRQLSYIQYQLYAKQKGLNFTRVSGIEGQDPSSNHGPDIKPQPSHTLSKDVLKSTQANHMANYTEKTDHQLDIRLRNGLHSIISEVDAEKSRVNGIHDDDDDDDIACSILPRGSQDTLLTNGHVHPVNGALKPQDFADHHPHVVDKASNQSHLPQDKYREGEEPCCSYTESKANSHLRSLISAASPAKQPGPHNMQTDLHQKPTSNSGTLGAPQKASLHDQTTVRLNGSYHVTSNSSTLANSSNQSGKSSAEAGIISEFFSHSRLHHISTWRNEFSEYVNSLQSRRRAAGGAVFSGREKLKKLKANCNSGSLMAAPQVRQSCVLHVDMDCFFVSVGIRHRPDLIGKPVAVTSNRGPGRVAQRPGANPQLEFQYYQNKQKHYRKEKTDGDLEMTPSPQDGEVPSNGVHEDLAALSMAEIASCSYEARQAGVRNGMFFGRAKQLCPDLQSVPYDFHAYKEVALAMYEILASYTHNIEALSCDEALVDATALLVELGVSPDELARSIREDIKEKTGCCASVGMSSNILLARMATRKAKPKGQYLLRSEEVDDFIRDQPVSSLPGVGRSMSSKLTSLGVSTCGDLQQLSLSQLQREFGPRTGQTLFRFCRGLDDRPVRSEKERKSVSAEMNYNIRFTQVEEAESFLNNLSMEVQKRLEGAGLRGRRVTLKVMMRKPGAPVEPAKYGGHGICDNFARSVLLAQPTDSGRVIASEAIKLFHAMKLNVKDMRGVGLQVQQLDGSHADPSGQGPSRGRSIRDLLLAKQSAHSPSKESPSQDGLISASSSRAFSSNQKRVTPALSPPPSTSSSSSPPPSFTIIDPMPGTSKGDYHHPHTPNHVRACLNISIEVPSPSQVDPSVLKALPIDIRRQVEETWRYREEQPSTSSHYSTPPRPPSTPPPGPSLVLQLPNQPGQPCTTGIILELPDFSQVDPDVFAALPRELQEELCSAYRNKGNAQAQASTVEQKNSFPQLKQPAVGKLKRRYKKRNTSPAKNGSSPLKKMFPGNSPAKTSPSKTLPLPLREQDLKCPSSLSTDVPETLPKPIPRPAPTLAGAHEFSEIRTLLREWVTTISEPMEEDILQVVKYCTELVEDKDLEKLYLVIKYMKRLMQQSSESVWSMAFDFVLDNVQVVVQQTYGSTLKIT

Target ORF information:

RefSeq Version	XM_017357713.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio REV1, polymerase (DNA directed) (rev1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357713.1

ORF Insert Sequence:

ATGAGTCGAG ATGGATGGAG GAAGAAAGCC AGTGAAAATG ATGGGTGGGG AGGACAGGGA 
GGTTACATGG CTGCAAAAGT CTCTAAGCTT GAGGAGCAGT TTCAGAAGGA TGCCCCGAGA 
GAGCAGAAGA AAGATGGAAA GTCCTCCTGC ATCTTCAGCG GTGTGGCCAT CTATGTCAAT 
GGATACACAG ATCCCAGTGC GGCTGAGTTA CGCAGGCTTA TGATGTTGCA TGGTGGGCAA 
TTTCATCTGT ATTACTCTCG CTCCAAAACC ACGCACATCA TTGCCACCAA CCTGCCCAAC 
TTCAAAATAC AAGAGCTCAA AGGAGAGAAA GTAGTGCGGC CAGAGTGGAT CATAGACAGT 
ATAAAAGCAG GTCGCCAACT ATCATATATT CAGTATCAGC TCTATGCGAA GCAGAAAGGA 
TTAAATTTCA CCAGAGTTTC TGGAATAGAA GGCCAGGACC CGTCATCCAA CCATGGACCA 
GACATCAAGC CTCAGCCGAG CCATACTCTA TCTAAAGACG TCCTCAAGTC TACTCAGGCT 
AACCACATGG CTAACTACAC TGAGAAAACC GATCACCAGC TGGATATCAG GCTCAGAAAT 
GGTTTGCATA GCATTATCTC TGAGGTAGAT GCAGAGAAAT CGAGAGTCAA TGGGATTCAT 
GATGATGATG ATGATGATGA CATCGCCTGT TCCATTTTGC CAAGAGGCTC ACAAGACACT 
TTGTTAACTA ACGGTCATGT ACACCCTGTG AATGGTGCCT TAAAGCCTCA GGACTTTGCA 
GACCATCATC CTCATGTTGT TGACAAAGCG TCCAATCAGT CTCACCTCCC ACAGGACAAA 
TACAGGGAGG GTGAGGAGCC CTGCTGCTCA TATACTGAAT CAAAAGCTAA TTCCCATCTG 
AGGTCCTTAA TATCAGCAGC CAGTCCTGCA AAGCAGCCTG GTCCTCACAA TATGCAAACG 
GACCTCCATC AAAAACCAAC ATCCAATTCA GGAACTTTAG GTGCTCCTCA GAAGGCCAGT 
CTGCATGATC AGACCACAGT CCGGCTAAAT GGGAGTTATC ACGTGACCTC AAACTCCTCC 
ACTCTGGCAA ACTCTAGTAA CCAGTCAGGA AAATCCTCTG CAGAGGCTGG AATAATATCC 
GAGTTCTTTT CTCACTCAAG GCTGCATCAC ATTTCTACGT GGCGCAATGA GTTCTCGGAG 
TATGTAAACA GCCTGCAGAG CAGACGACGA GCAGCAGGTG GAGCTGTTTT CTCTGGAAGA 
GAGAAGCTGA AGAAGCTCAA AGCTAACTGC AATTCAGGTT CCTTGATGGC TGCTCCTCAG 
GTGCGTCAGT CATGTGTTCT CCATGTTGAT ATGGACTGCT TCTTTGTCTC TGTGGGGATT 
AGACACCGAC CAGATCTTAT AGGGAAACCA GTTGCGGTGA CTAGTAATCG TGGTCCAGGC 
CGTGTTGCTC AGCGCCCTGG TGCAAACCCT CAGCTTGAGT TTCAGTATTA CCAGAATAAA 
CAGAAACATT ACAGGAAAGA GAAGACAGAT GGGGATCTTG AGATGACCCC ATCTCCACAG 
GATGGTGAAG TTCCCAGTAA TGGAGTGCAT GAAGACCTGG CTGCCCTTTC TATGGCAGAG 
ATTGCCTCTT GTAGTTACGA GGCCAGGCAG GCTGGTGTGC GAAATGGCAT GTTCTTTGGT 
AGGGCCAAGC AGCTGTGTCC AGATCTGCAG TCTGTTCCAT ATGACTTCCA TGCTTATAAA 
GAGGTGGCTC TGGCCATGTA TGAGATCCTG GCCAGTTACA CTCATAACAT TGAGGCGCTG 
AGCTGTGACG AGGCACTGGT TGATGCCACA GCTCTTCTGG TTGAGCTGGG GGTGTCTCCT 
GATGAACTGG CACGGTCCAT CCGTGAAGAC ATAAAGGAGA AGACTGGCTG CTGTGCCTCA 
GTTGGAATGA GTTCCAACAT CCTACTTGCT CGGATGGCAA CCCGTAAGGC TAAACCCAAA 
GGGCAGTATT TACTCAGATC AGAGGAAGTG GATGATTTTA TTAGAGACCA GCCTGTGTCC 
AGTTTACCTG GTGTGGGTCG CTCAATGAGC TCCAAGTTGA CCTCTCTAGG AGTGAGTACC 
TGCGGTGACC TGCAGCAGTT GTCCCTGTCT CAACTGCAGA GGGAGTTTGG GCCACGGACG 
GGACAGACGC TCTTTCGCTT TTGCAGGGGA CTAGATGACA GACCTGTGCG CAGTGAGAAA 
GAGAGAAAGT CTGTGTCTGC GGAGATGAAC TACAACATTC GGTTCACACA GGTTGAGGAG 
GCGGAGTCTT TCCTCAATAA TCTCTCTATG GAGGTACAGA AGAGGTTAGA GGGGGCGGGG 
CTTAGAGGTC GTCGGGTTAC ACTGAAGGTC ATGATGCGGA AACCAGGTGC GCCGGTAGAA 
CCAGCCAAAT ATGGTGGTCA TGGGATATGT GACAACTTTG CCAGGTCTGT TTTACTAGCT 
CAGCCCACAG ACAGTGGTCG AGTGATTGCA TCAGAGGCCA TTAAGCTGTT TCATGCCATG 
AAGCTGAATG TGAAGGACAT GAGGGGAGTG GGGCTGCAGG TGCAGCAGCT GGATGGATCA 
CATGCAGACC CTTCAGGACA AGGGCCCTCT CGTGGCCGAT CAATCAGAGA CCTGCTTCTG 
GCCAAACAGT CTGCCCACAG CCCCAGCAAG GAGTCTCCAT CACAAGATGG TTTAATCAGC 
GCTTCGTCAT CCAGAGCTTT CTCCTCCAAT CAGAAGAGAG TTACCCCTGC ATTATCACCG 
CCACCTTCTA CATCATCATC ATCATCACCA CCACCCTCCT TCACCATTAT AGATCCAATG 
CCTGGAACAA GTAAAGGAGA TTATCACCAT CCACACACCC CCAACCACGT CAGGGCCTGC 
CTAAACATCA GCATCGAGGT GCCATCTCCC TCTCAGGTGG ACCCATCTGT TTTAAAAGCT 
TTACCTATAG ACATAAGGAG ACAAGTAGAG GAAACCTGGA GATACAGAGA GGAGCAGCCC 
AGCACTTCTT CTCATTACTC AACACCACCT CGACCACCCT CCACTCCACC TCCAGGACCT 
TCACTTGTTC TGCAGCTCCC AAACCAGCCT GGTCAACCAT GCACAACGGG CATTATATTA 
GAGCTCCCGG ACTTCTCCCA GGTTGACCCA GATGTGTTTG CAGCACTCCC TAGAGAGCTT 
CAGGAAGAGT TGTGTTCTGC TTACAGAAAT AAGGGAAATG CTCAAGCTCA AGCCAGCACT 
GTGGAACAAA AAAACTCATT TCCTCAACTG AAGCAGCCTG CTGTAGGGAA ATTAAAGCGC 
CGCTACAAAA AAAGAAACAC TAGTCCTGCC AAAAATGGTT CAAGTCCACT AAAAAAAATG 
TTCCCTGGGA ACAGCCCTGC CAAAACCAGC CCCTCCAAAA CTCTTCCTCT GCCACTCAGA 
GAACAAGACC TCAAATGCCC GTCCTCTCTG AGCACAGATG TCCCAGAGAC ACTACCCAAA 
CCCATTCCTC GGCCAGCCCC AACCCTTGCA GGAGCGCATG AATTCAGTGA GATAAGAACG 
CTGCTGCGTG AATGGGTCAC CACCATCTCA GAACCAATGG AGGAGGACAT TTTACAGGTG 
GTGAAGTACT GCACAGAACT AGTGGAGGAC AAAGACCTGG AGAAACTCTA CCTTGTCATC 
AAGTACATGA AGAGGCTCAT GCAGCAGTCC TCAGAGTCAG TGTGGAGCAT GGCCTTTGAC 
TTTGTCCTGG ACAATGTGCA GGTGGTGGTA CAGCAAACCT ACGGCAGCAC TTTGAAGATC 
ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

REV1 ( XM_017357714.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017213203.1 Danio rerio REV1, polymerase (DNA directed) (rev1), transcript variant X4, mRNA.

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CloneID	ODa60271
Clone ID Related Accession (Same CDS sequence)	XM_017357714.1 , XM_017357714.2
Accession Version	XM_017357714.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	DNA repair protein REV1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCATC CCAGTGCGGC TGAGTTACGC AGGCTTATGA TGTTGCATGG TGGGCAATTT 
CATCTGTATT ACTCTCGCTC CAAAACCACG CACATCATTG CCACCAACCT GCCCAACTTC 
AAAATACAAG AGCTCAAAGG AGAGAAAGTA GTGCGGCCAG AGTGGATCAT AGACAGTATA 
AAAGCAGGTC GCCAACTATC ATATATTCAG TATCAGCTCT ATGCGAAGCA GAAAGGATTA 
AATTTCACCA GAGTTTCTGG AATAGAAGGC CAGGACCCGT CATCCAACCA TGGACCAGAC 
ATCAAGCCTC AGCCGAGCCA TACTCTATCT AAAGACGTCC TCAAGTCTAC TCAGGCTAAC 
CACATGGCTA ACTACACTGA GAAAACCGAT CACCAGCTGG ATATCAGGCT CAGAAATGGT 
TTGCATAGCA TTATCTCTGA GGTAGATGCA GAGAAATCGA GAGTCAATGG GATTCATGAT 
GATGATGATG ATGATGACAT CGCCTGTTCC ATTTTGCCAA GAGGCTCACA AGACACTTTG 
TTAACTAACG GTCATGTACA CCCTGTGAAT GGTGCCTTAA AGCCTCAGGA CTTTGCAGAC 
CATCATCCTC ATGTTGTTGA CAAAGCGTCC AATCAGTCTC ACCTCCCACA GGACAAATAC 
AGGGAGGGTG AGGAGCCCTG CTGCTCATAT ACTGAATCAA AAGCTAATTC CCATCTGAGG 
TCCTTAATAT CAGCAGCCAG TCCTGCAAAG CAGCCTGGTC CTCACAATAT GCAAACGGAC 
CTCCATCAAA AACCAACATC CAATTCAGGA ACTTTAGGTG CTCCTCAGAA GGCCAGTCTG 
CATGATCAGA CCACAGTCCG GCTAAATGGG AGTTATCACG TGACCTCAAA CTCCTCCACT 
CTGGCAAACT CTAGTAACCA GTCAGGAAAA TCCTCTGCAG AGGCTGGAAT AATATCCGAG 
TTCTTTTCTC ACTCAAGGCT GCATCACATT TCTACGTGGC GCAATGAGTT CTCGGAGTAT 
GTAAACAGCC TGCAGAGCAG ACGACGAGCA GCAGGTGGAG CTGTTTTCTC TGGAAGAGAG 
AAGCTGAAGA AGCTCAAAGC TAACTGCAAT TCAGGTTCCT TGATGGCTGC TCCTCAGGTG 
CGTCAGTCAT GTGTTCTCCA TGTTGATATG GACTGCTTCT TTGTCTCTGT GGGGATTAGA 
CACCGACCAG ATCTTATAGG GAAACCAGTT GCGGTGACTA GTAATCGTGG TCCAGGCCGT 
GTTGCTCAGC GCCCTGGTGC AAACCCTCAG CTTGAGTTTC AGTATTACCA GAATAAACAG 
AAACATTACA GGAAAGAGAA GACAGATGGG GATCTTGAGA TGACCCCATC TCCACAGGAT 
GGTGAAGTTC CCAGTAATGG AGTGCATGAA GACCTGGCTG CCCTTTCTAT GGCAGAGATT 
GCCTCTTGTA GTTACGAGGC CAGGCAGGCT GGTGTGCGAA ATGGCATGTT CTTTGGTAGG 
GCCAAGCAGC TGTGTCCAGA TCTGCAGTCT GTTCCATATG ACTTCCATGC TTATAAAGAG 
GTGGCTCTGG CCATGTATGA GATCCTGGCC AGTTACACTC ATAACATTGA GGCGCTGAGC 
TGTGACGAGG CACTGGTTGA TGCCACAGCT CTTCTGGTTG AGCTGGGGGT GTCTCCTGAT 
GAACTGGCAC GGTCCATCCG TGAAGACATA AAGGAGAAGA CTGGCTGCTG TGCCTCAGTT 
GGAATGAGTT CCAACATCCT ACTTGCTCGG ATGGCAACCC GTAAGGCTAA ACCCAAAGGG 
CAGTATTTAC TCAGATCAGA GGAAGTGGAT GATTTTATTA GAGACCAGCC TGTGTCCAGT 
TTACCTGGTG TGGGTCGCTC AATGAGCTCC AAGTTGACCT CTCTAGGAGT GAGTACCTGC 
GGTGACCTGC AGCAGTTGTC CCTGTCTCAA CTGCAGAGGG AGTTTGGGCC ACGGACGGGA 
CAGACGCTCT TTCGCTTTTG CAGGGGACTA GATGACAGAC CTGTGCGCAG TGAGAAAGAG 
AGAAAGTCTG TGTCTGCGGA GATGAACTAC AACATTCGGT TCACACAGGT TGAGGAGGCG 
GAGTCTTTCC TCAATAATCT CTCTATGGAG GTACAGAAGA GGTTAGAGGG GGCGGGGCTT 
AGAGGTCGTC GGGTTACACT GAAGGTCATG ATGCGGAAAC CAGGTGCGCC GGTAGAACCA 
GCCAAATATG GTGGTCATGG GATATGTGAC AACTTTGCCA GGTCTGTTTT ACTAGCTCAG 
CCCACAGACA GTGGTCGAGT GATTGCATCA GAGGCCATTA AGCTGTTTCA TGCCATGAAG 
CTGAATGTGA AGGACATGAG GGGAGTGGGG CTGCAGGTGC AGCAGCTGGA TGGATCACAT 
GCAGACCCTT CAGGACAAGG GCCCTCTCGT GGCCGATCAA TCAGAGACCT GCTTCTGGCC 
AAACAGTCTG CCCACAGCCC CAGCAAGGAG TCTCCATCAC AAGATGGTTT AATCAGCGCT 
TCGTCATCCA GAGCTTTCTC CTCCAATCAG AAGAGAGTTA CCCCTGCATT ATCACCGCCA 
CCTTCTACAT CATCATCATC ATCACCACCA CCCTCCTTCA CCATTATAGA TCCAATGCCT 
GGAACAAGTA AAGGAGATTA TCACCATCCA CACACCCCCA ACCACGTCAG GGCCTGCCTA 
AACATCAGCA TCGAGGTGCC ATCTCCCTCT CAGGTGGACC CATCTGTTTT AAAAGCTTTA 
CCTATAGACA TAAGGAGACA AGTAGAGGAA ACCTGGAGAT ACAGAGAGGA GCAGCCCAGC 
ACTTCTTCTC ATTACTCAAC ACCACCTCGA CCACCCTCCA CTCCACCTCC AGGACCTTCA 
CTTGTTCTGC AGCTCCCAAA CCAGCCTGGT CAACCATGCA CAACGGGCAT TATATTAGAG 
CTCCCGGACT TCTCCCAGGT TGACCCAGAT GTGTTTGCAG CACTCCCTAG AGAGCTTCAG 
GAAGAGTTGT GTTCTGCTTA CAGAAATAAG GGAAATGCTC AAGCTCAAGC CAGCACTGTA 
GTGGAACAAA AAAACTCATT TCCTCAACTG AAGCAGCCTG CTGTAGGGAA ATTAAAGCGC 
CGCTACAAAA AAAGAAACAC TAGTCCTGCC AAAAATGGTT CAAGTCCACT AAAAAAAATG 
TTCCCTGGGA ACAGCCCTGC CAAAACCAGC CCCTCCAAAA CTCTTCCTCT GCCACTCAGA 
GAACAAGACC TCAAATGCCC GTCCTCTCTG AGCACAGATG TCCCAGAGAC ACTACCCAAA 
CCCATTCCTC GGCCAGCCCC AACCCTTGCA GGAGCGCATG AATTCAGTGA GATAAGAACG 
CTGCTGCGTG AATGGGTCAC CACCATCTCA GAACCAATGG AGGAGGACAT TTTACAGGTG 
GTGAAGTACT GCACAGAACT AGTGGAGGAC AAAGACCTGG AGAAACTCTA CCTTGTCATC 
AAGTACATGA AGAGGCTCAT GCAGCAGTCC TCAGAGTCAG TGTGGAGCAT GGCCTTTGAC 
TTTGTCCTGG ACAATGTGCA GGTGGTGGTA CAGCAAACCT ACGGCAGCAC TTTGAAGATC 
ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213203.1
CDS	117..3722
Translation	MFHPSAAELRRLMMLHGGQFHLYYSRSKTTHIIATNLPNFKIQELKGEKVVRPEWIIDSIKAGRQLSYIQYQLYAKQKGLNFTRVSGIEGQDPSSNHGPDIKPQPSHTLSKDVLKSTQANHMANYTEKTDHQLDIRLRNGLHSIISEVDAEKSRVNGIHDDDDDDDIACSILPRGSQDTLLTNGHVHPVNGALKPQDFADHHPHVVDKASNQSHLPQDKYREGEEPCCSYTESKANSHLRSLISAASPAKQPGPHNMQTDLHQKPTSNSGTLGAPQKASLHDQTTVRLNGSYHVTSNSSTLANSSNQSGKSSAEAGIISEFFSHSRLHHISTWRNEFSEYVNSLQSRRRAAGGAVFSGREKLKKLKANCNSGSLMAAPQVRQSCVLHVDMDCFFVSVGIRHRPDLIGKPVAVTSNRGPGRVAQRPGANPQLEFQYYQNKQKHYRKEKTDGDLEMTPSPQDGEVPSNGVHEDLAALSMAEIASCSYEARQAGVRNGMFFGRAKQLCPDLQSVPYDFHAYKEVALAMYEILASYTHNIEALSCDEALVDATALLVELGVSPDELARSIREDIKEKTGCCASVGMSSNILLARMATRKAKPKGQYLLRSEEVDDFIRDQPVSSLPGVGRSMSSKLTSLGVSTCGDLQQLSLSQLQREFGPRTGQTLFRFCRGLDDRPVRSEKERKSVSAEMNYNIRFTQVEEAESFLNNLSMEVQKRLEGAGLRGRRVTLKVMMRKPGAPVEPAKYGGHGICDNFARSVLLAQPTDSGRVIASEAIKLFHAMKLNVKDMRGVGLQVQQLDGSHADPSGQGPSRGRSIRDLLLAKQSAHSPSKESPSQDGLISASSSRAFSSNQKRVTPALSPPPSTSSSSSPPPSFTIIDPMPGTSKGDYHHPHTPNHVRACLNISIEVPSPSQVDPSVLKALPIDIRRQVEETWRYREEQPSTSSHYSTPPRPPSTPPPGPSLVLQLPNQPGQPCTTGIILELPDFSQVDPDVFAALPRELQEELCSAYRNKGNAQAQASTVVEQKNSFPQLKQPAVGKLKRRYKKRNTSPAKNGSSPLKKMFPGNSPAKTSPSKTLPLPLREQDLKCPSSLSTDVPETLPKPIPRPAPTLAGAHEFSEIRTLLREWVTTISEPMEEDILQVVKYCTELVEDKDLEKLYLVIKYMKRLMQQSSESVWSMAFDFVLDNVQVVVQQTYGSTLKIT

Target ORF information:

RefSeq Version	XM_017357714.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio REV1, polymerase (DNA directed) (rev1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357714.1

ORF Insert Sequence:

ATGTTCCATC CCAGTGCGGC TGAGTTACGC AGGCTTATGA TGTTGCATGG TGGGCAATTT 
CATCTGTATT ACTCTCGCTC CAAAACCACG CACATCATTG CCACCAACCT GCCCAACTTC 
AAAATACAAG AGCTCAAAGG AGAGAAAGTA GTGCGGCCAG AGTGGATCAT AGACAGTATA 
AAAGCAGGTC GCCAACTATC ATATATTCAG TATCAGCTCT ATGCGAAGCA GAAAGGATTA 
AATTTCACCA GAGTTTCTGG AATAGAAGGC CAGGACCCGT CATCCAACCA TGGACCAGAC 
ATCAAGCCTC AGCCGAGCCA TACTCTATCT AAAGACGTCC TCAAGTCTAC TCAGGCTAAC 
CACATGGCTA ACTACACTGA GAAAACCGAT CACCAGCTGG ATATCAGGCT CAGAAATGGT 
TTGCATAGCA TTATCTCTGA GGTAGATGCA GAGAAATCGA GAGTCAATGG GATTCATGAT 
GATGATGATG ATGATGACAT CGCCTGTTCC ATTTTGCCAA GAGGCTCACA AGACACTTTG 
TTAACTAACG GTCATGTACA CCCTGTGAAT GGTGCCTTAA AGCCTCAGGA CTTTGCAGAC 
CATCATCCTC ATGTTGTTGA CAAAGCGTCC AATCAGTCTC ACCTCCCACA GGACAAATAC 
AGGGAGGGTG AGGAGCCCTG CTGCTCATAT ACTGAATCAA AAGCTAATTC CCATCTGAGG 
TCCTTAATAT CAGCAGCCAG TCCTGCAAAG CAGCCTGGTC CTCACAATAT GCAAACGGAC 
CTCCATCAAA AACCAACATC CAATTCAGGA ACTTTAGGTG CTCCTCAGAA GGCCAGTCTG 
CATGATCAGA CCACAGTCCG GCTAAATGGG AGTTATCACG TGACCTCAAA CTCCTCCACT 
CTGGCAAACT CTAGTAACCA GTCAGGAAAA TCCTCTGCAG AGGCTGGAAT AATATCCGAG 
TTCTTTTCTC ACTCAAGGCT GCATCACATT TCTACGTGGC GCAATGAGTT CTCGGAGTAT 
GTAAACAGCC TGCAGAGCAG ACGACGAGCA GCAGGTGGAG CTGTTTTCTC TGGAAGAGAG 
AAGCTGAAGA AGCTCAAAGC TAACTGCAAT TCAGGTTCCT TGATGGCTGC TCCTCAGGTG 
CGTCAGTCAT GTGTTCTCCA TGTTGATATG GACTGCTTCT TTGTCTCTGT GGGGATTAGA 
CACCGACCAG ATCTTATAGG GAAACCAGTT GCGGTGACTA GTAATCGTGG TCCAGGCCGT 
GTTGCTCAGC GCCCTGGTGC AAACCCTCAG CTTGAGTTTC AGTATTACCA GAATAAACAG 
AAACATTACA GGAAAGAGAA GACAGATGGG GATCTTGAGA TGACCCCATC TCCACAGGAT 
GGTGAAGTTC CCAGTAATGG AGTGCATGAA GACCTGGCTG CCCTTTCTAT GGCAGAGATT 
GCCTCTTGTA GTTACGAGGC CAGGCAGGCT GGTGTGCGAA ATGGCATGTT CTTTGGTAGG 
GCCAAGCAGC TGTGTCCAGA TCTGCAGTCT GTTCCATATG ACTTCCATGC TTATAAAGAG 
GTGGCTCTGG CCATGTATGA GATCCTGGCC AGTTACACTC ATAACATTGA GGCGCTGAGC 
TGTGACGAGG CACTGGTTGA TGCCACAGCT CTTCTGGTTG AGCTGGGGGT GTCTCCTGAT 
GAACTGGCAC GGTCCATCCG TGAAGACATA AAGGAGAAGA CTGGCTGCTG TGCCTCAGTT 
GGAATGAGTT CCAACATCCT ACTTGCTCGG ATGGCAACCC GTAAGGCTAA ACCCAAAGGG 
CAGTATTTAC TCAGATCAGA GGAAGTGGAT GATTTTATTA GAGACCAGCC TGTGTCCAGT 
TTACCTGGTG TGGGTCGCTC AATGAGCTCC AAGTTGACCT CTCTAGGAGT GAGTACCTGC 
GGTGACCTGC AGCAGTTGTC CCTGTCTCAA CTGCAGAGGG AGTTTGGGCC ACGGACGGGA 
CAGACGCTCT TTCGCTTTTG CAGGGGACTA GATGACAGAC CTGTGCGCAG TGAGAAAGAG 
AGAAAGTCTG TGTCTGCGGA GATGAACTAC AACATTCGGT TCACACAGGT TGAGGAGGCG 
GAGTCTTTCC TCAATAATCT CTCTATGGAG GTACAGAAGA GGTTAGAGGG GGCGGGGCTT 
AGAGGTCGTC GGGTTACACT GAAGGTCATG ATGCGGAAAC CAGGTGCGCC GGTAGAACCA 
GCCAAATATG GTGGTCATGG GATATGTGAC AACTTTGCCA GGTCTGTTTT ACTAGCTCAG 
CCCACAGACA GTGGTCGAGT GATTGCATCA GAGGCCATTA AGCTGTTTCA TGCCATGAAG 
CTGAATGTGA AGGACATGAG GGGAGTGGGG CTGCAGGTGC AGCAGCTGGA TGGATCACAT 
GCAGACCCTT CAGGACAAGG GCCCTCTCGT GGCCGATCAA TCAGAGACCT GCTTCTGGCC 
AAACAGTCTG CCCACAGCCC CAGCAAGGAG TCTCCATCAC AAGATGGTTT AATCAGCGCT 
TCGTCATCCA GAGCTTTCTC CTCCAATCAG AAGAGAGTTA CCCCTGCATT ATCACCGCCA 
CCTTCTACAT CATCATCATC ATCACCACCA CCCTCCTTCA CCATTATAGA TCCAATGCCT 
GGAACAAGTA AAGGAGATTA TCACCATCCA CACACCCCCA ACCACGTCAG GGCCTGCCTA 
AACATCAGCA TCGAGGTGCC ATCTCCCTCT CAGGTGGACC CATCTGTTTT AAAAGCTTTA 
CCTATAGACA TAAGGAGACA AGTAGAGGAA ACCTGGAGAT ACAGAGAGGA GCAGCCCAGC 
ACTTCTTCTC ATTACTCAAC ACCACCTCGA CCACCCTCCA CTCCACCTCC AGGACCTTCA 
CTTGTTCTGC AGCTCCCAAA CCAGCCTGGT CAACCATGCA CAACGGGCAT TATATTAGAG 
CTCCCGGACT TCTCCCAGGT TGACCCAGAT GTGTTTGCAG CACTCCCTAG AGAGCTTCAG 
GAAGAGTTGT GTTCTGCTTA CAGAAATAAG GGAAATGCTC AAGCTCAAGC CAGCACTGTA 
GTGGAACAAA AAAACTCATT TCCTCAACTG AAGCAGCCTG CTGTAGGGAA ATTAAAGCGC 
CGCTACAAAA AAAGAAACAC TAGTCCTGCC AAAAATGGTT CAAGTCCACT AAAAAAAATG 
TTCCCTGGGA ACAGCCCTGC CAAAACCAGC CCCTCCAAAA CTCTTCCTCT GCCACTCAGA 
GAACAAGACC TCAAATGCCC GTCCTCTCTG AGCACAGATG TCCCAGAGAC ACTACCCAAA 
CCCATTCCTC GGCCAGCCCC AACCCTTGCA GGAGCGCATG AATTCAGTGA GATAAGAACG 
CTGCTGCGTG AATGGGTCAC CACCATCTCA GAACCAATGG AGGAGGACAT TTTACAGGTG 
GTGAAGTACT GCACAGAACT AGTGGAGGAC AAAGACCTGG AGAAACTCTA CCTTGTCATC 
AAGTACATGA AGAGGCTCAT GCAGCAGTCC TCAGAGTCAG TGTGGAGCAT GGCCTTTGAC 
TTTGTCCTGG ACAATGTGCA GGTGGTGGTA CAGCAAACCT ACGGCAGCAC TTTGAAGATC 
ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

REV1 ( XM_017357714.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017213203.1 Danio rerio REV1, polymerase (DNA directed) (rev1), transcript variant X3, mRNA.

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CloneID	ODa60271
Clone ID Related Accession (Same CDS sequence)	XM_017357714.1 , XM_017357714.2
Accession Version	XM_017357714.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	DNA repair protein REV1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017357714.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCATC CCAGTGCGGC TGAGTTACGC AGGCTTATGA TGTTGCATGG TGGGCAATTT 
CATCTGTATT ACTCTCGCTC CAAAACCACG CACATCATTG CCACCAACCT GCCCAACTTC 
AAAATACAAG AGCTCAAAGG AGAGAAAGTA GTGCGGCCAG AGTGGATCAT AGACAGTATA 
AAAGCAGGTC GCCAACTATC ATATATTCAG TATCAGCTCT ATGCGAAGCA GAAAGGATTA 
AATTTCACCA GAGTTTCTGG AATAGAAGGC CAGGACCCGT CATCCAACCA TGGACCAGAC 
ATCAAGCCTC AGCCGAGCCA TACTCTATCT AAAGACGTCC TCAAGTCTAC TCAGGCTAAC 
CACATGGCTA ACTACACTGA GAAAACCGAT CACCAGCTGG ATATCAGGCT CAGAAATGGT 
TTGCATAGCA TTATCTCTGA GGTAGATGCA GAGAAATCGA GAGTCAATGG GATTCATGAT 
GATGATGATG ATGATGACAT CGCCTGTTCC ATTTTGCCAA GAGGCTCACA AGACACTTTG 
TTAACTAACG GTCATGTACA CCCTGTGAAT GGTGCCTTAA AGCCTCAGGA CTTTGCAGAC 
CATCATCCTC ATGTTGTTGA CAAAGCGTCC AATCAGTCTC ACCTCCCACA GGACAAATAC 
AGGGAGGGTG AGGAGCCCTG CTGCTCATAT ACTGAATCAA AAGCTAATTC CCATCTGAGG 
TCCTTAATAT CAGCAGCCAG TCCTGCAAAG CAGCCTGGTC CTCACAATAT GCAAACGGAC 
CTCCATCAAA AACCAACATC CAATTCAGGA ACTTTAGGTG CTCCTCAGAA GGCCAGTCTG 
CATGATCAGA CCACAGTCCG GCTAAATGGG AGTTATCACG TGACCTCAAA CTCCTCCACT 
CTGGCAAACT CTAGTAACCA GTCAGGAAAA TCCTCTGCAG AGGCTGGAAT AATATCCGAG 
TTCTTTTCTC ACTCAAGGCT GCATCACATT TCTACGTGGC GCAATGAGTT CTCGGAGTAT 
GTAAACAGCC TGCAGAGCAG ACGACGAGCA GCAGGTGGAG CTGTTTTCTC TGGAAGAGAG 
AAGCTGAAGA AGCTCAAAGC TAACTGCAAT TCAGGTTCCT TGATGGCTGC TCCTCAGGTG 
CGTCAGTCAT GTGTTCTCCA TGTTGATATG GACTGCTTCT TTGTCTCTGT GGGGATTAGA 
CACCGACCAG ATCTTATAGG GAAACCAGTT GCGGTGACTA GTAATCGTGG TCCAGGCCGT 
GTTGCTCAGC GCCCTGGTGC AAACCCTCAG CTTGAGTTTC AGTATTACCA GAATAAACAG 
AAACATTACA GGAAAGAGAA GACAGATGGG GATCTTGAGA TGACCCCATC TCCACAGGAT 
GGTGAAGTTC CCAGTAATGG AGTGCATGAA GACCTGGCTG CCCTTTCTAT GGCAGAGATT 
GCCTCTTGTA GTTACGAGGC CAGGCAGGCT GGTGTGCGAA ATGGCATGTT CTTTGGTAGG 
GCCAAGCAGC TGTGTCCAGA TCTGCAGTCT GTTCCATATG ACTTCCATGC TTATAAAGAG 
GTGGCTCTGG CCATGTATGA GATCCTGGCC AGTTACACTC ATAACATTGA GGCGCTGAGC 
TGTGACGAGG CACTGGTTGA TGCCACAGCT CTTCTGGTTG AGCTGGGGGT GTCTCCTGAT 
GAACTGGCAC GGTCCATCCG TGAAGACATA AAGGAGAAGA CTGGCTGCTG TGCCTCAGTT 
GGAATGAGTT CCAACATCCT ACTTGCTCGG ATGGCAACCC GTAAGGCTAA ACCCAAAGGG 
CAGTATTTAC TCAGATCAGA GGAAGTGGAT GATTTTATTA GAGACCAGCC TGTGTCCAGT 
TTACCTGGTG TGGGTCGCTC AATGAGCTCC AAGTTGACCT CTCTAGGAGT GAGTACCTGC 
GGTGACCTGC AGCAGTTGTC CCTGTCTCAA CTGCAGAGGG AGTTTGGGCC ACGGACGGGA 
CAGACGCTCT TTCGCTTTTG CAGGGGACTA GATGACAGAC CTGTGCGCAG TGAGAAAGAG 
AGAAAGTCTG TGTCTGCGGA GATGAACTAC AACATTCGGT TCACACAGGT TGAGGAGGCG 
GAGTCTTTCC TCAATAATCT CTCTATGGAG GTACAGAAGA GGTTAGAGGG GGCGGGGCTT 
AGAGGTCGTC GGGTTACACT GAAGGTCATG ATGCGGAAAC CAGGTGCGCC GGTAGAACCA 
GCCAAATATG GTGGTCATGG GATATGTGAC AACTTTGCCA GGTCTGTTTT ACTAGCTCAG 
CCCACAGACA GTGGTCGAGT GATTGCATCA GAGGCCATTA AGCTGTTTCA TGCCATGAAG 
CTGAATGTGA AGGACATGAG GGGAGTGGGG CTGCAGGTGC AGCAGCTGGA TGGATCACAT 
GCAGACCCTT CAGGACAAGG GCCCTCTCGT GGCCGATCAA TCAGAGACCT GCTTCTGGCC 
AAACAGTCTG CCCACAGCCC CAGCAAGGAG TCTCCATCAC AAGATGGTTT AATCAGCGCT 
TCGTCATCCA GAGCTTTCTC CTCCAATCAG AAGAGAGTTA CCCCTGCATT ATCACCGCCA 
CCTTCTACAT CATCATCATC ATCACCACCA CCCTCCTTCA CCATTATAGA TCCAATGCCT 
GGAACAAGTA AAGGAGATTA TCACCATCCA CACACCCCCA ACCACGTCAG GGCCTGCCTA 
AACATCAGCA TCGAGGTGCC ATCTCCCTCT CAGGTGGACC CATCTGTTTT AAAAGCTTTA 
CCTATAGACA TAAGGAGACA AGTAGAGGAA ACCTGGAGAT ACAGAGAGGA GCAGCCCAGC 
ACTTCTTCTC ATTACTCAAC ACCACCTCGA CCACCCTCCA CTCCACCTCC AGGACCTTCA 
CTTGTTCTGC AGCTCCCAAA CCAGCCTGGT CAACCATGCA CAACGGGCAT TATATTAGAG 
CTCCCGGACT TCTCCCAGGT TGACCCAGAT GTGTTTGCAG CACTCCCTAG AGAGCTTCAG 
GAAGAGTTGT GTTCTGCTTA CAGAAATAAG GGAAATGCTC AAGCTCAAGC CAGCACTGTA 
GTGGAACAAA AAAACTCATT TCCTCAACTG AAGCAGCCTG CTGTAGGGAA ATTAAAGCGC 
CGCTACAAAA AAAGAAACAC TAGTCCTGCC AAAAATGGTT CAAGTCCACT AAAAAAAATG 
TTCCCTGGGA ACAGCCCTGC CAAAACCAGC CCCTCCAAAA CTCTTCCTCT GCCACTCAGA 
GAACAAGACC TCAAATGCCC GTCCTCTCTG AGCACAGATG TCCCAGAGAC ACTACCCAAA 
CCCATTCCTC GGCCAGCCCC AACCCTTGCA GGAGCGCATG AATTCAGTGA GATAAGAACG 
CTGCTGCGTG AATGGGTCAC CACCATCTCA GAACCAATGG AGGAGGACAT TTTACAGGTG 
GTGAAGTACT GCACAGAACT AGTGGAGGAC AAAGACCTGG AGAAACTCTA CCTTGTCATC 
AAGTACATGA AGAGGCTCAT GCAGCAGTCC TCAGAGTCAG TGTGGAGCAT GGCCTTTGAC 
TTTGTCCTGG ACAATGTGCA GGTGGTGGTA CAGCAAACCT ACGGCAGCAC TTTGAAGATC 
ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213203.1
CDS	117..3722
Translation	MFHPSAAELRRLMMLHGGQFHLYYSRSKTTHIIATNLPNFKIQELKGEKVVRPEWIIDSIKAGRQLSYIQYQLYAKQKGLNFTRVSGIEGQDPSSNHGPDIKPQPSHTLSKDVLKSTQANHMANYTEKTDHQLDIRLRNGLHSIISEVDAEKSRVNGIHDDDDDDDIACSILPRGSQDTLLTNGHVHPVNGALKPQDFADHHPHVVDKASNQSHLPQDKYREGEEPCCSYTESKANSHLRSLISAASPAKQPGPHNMQTDLHQKPTSNSGTLGAPQKASLHDQTTVRLNGSYHVTSNSSTLANSSNQSGKSSAEAGIISEFFSHSRLHHISTWRNEFSEYVNSLQSRRRAAGGAVFSGREKLKKLKANCNSGSLMAAPQVRQSCVLHVDMDCFFVSVGIRHRPDLIGKPVAVTSNRGPGRVAQRPGANPQLEFQYYQNKQKHYRKEKTDGDLEMTPSPQDGEVPSNGVHEDLAALSMAEIASCSYEARQAGVRNGMFFGRAKQLCPDLQSVPYDFHAYKEVALAMYEILASYTHNIEALSCDEALVDATALLVELGVSPDELARSIREDIKEKTGCCASVGMSSNILLARMATRKAKPKGQYLLRSEEVDDFIRDQPVSSLPGVGRSMSSKLTSLGVSTCGDLQQLSLSQLQREFGPRTGQTLFRFCRGLDDRPVRSEKERKSVSAEMNYNIRFTQVEEAESFLNNLSMEVQKRLEGAGLRGRRVTLKVMMRKPGAPVEPAKYGGHGICDNFARSVLLAQPTDSGRVIASEAIKLFHAMKLNVKDMRGVGLQVQQLDGSHADPSGQGPSRGRSIRDLLLAKQSAHSPSKESPSQDGLISASSSRAFSSNQKRVTPALSPPPSTSSSSSPPPSFTIIDPMPGTSKGDYHHPHTPNHVRACLNISIEVPSPSQVDPSVLKALPIDIRRQVEETWRYREEQPSTSSHYSTPPRPPSTPPPGPSLVLQLPNQPGQPCTTGIILELPDFSQVDPDVFAALPRELQEELCSAYRNKGNAQAQASTVVEQKNSFPQLKQPAVGKLKRRYKKRNTSPAKNGSSPLKKMFPGNSPAKTSPSKTLPLPLREQDLKCPSSLSTDVPETLPKPIPRPAPTLAGAHEFSEIRTLLREWVTTISEPMEEDILQVVKYCTELVEDKDLEKLYLVIKYMKRLMQQSSESVWSMAFDFVLDNVQVVVQQTYGSTLKIT

Target ORF information:

RefSeq Version	XM_017357714.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio REV1, polymerase (DNA directed) (rev1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357714.2

ORF Insert Sequence:

ATGTTCCATC CCAGTGCGGC TGAGTTACGC AGGCTTATGA TGTTGCATGG TGGGCAATTT 
CATCTGTATT ACTCTCGCTC CAAAACCACG CACATCATTG CCACCAACCT GCCCAACTTC 
AAAATACAAG AGCTCAAAGG AGAGAAAGTA GTGCGGCCAG AGTGGATCAT AGACAGTATA 
AAAGCAGGTC GCCAACTATC ATATATTCAG TATCAGCTCT ATGCGAAGCA GAAAGGATTA 
AATTTCACCA GAGTTTCTGG AATAGAAGGC CAGGACCCGT CATCCAACCA TGGACCAGAC 
ATCAAGCCTC AGCCGAGCCA TACTCTATCT AAAGACGTCC TCAAGTCTAC TCAGGCTAAC 
CACATGGCTA ACTACACTGA GAAAACCGAT CACCAGCTGG ATATCAGGCT CAGAAATGGT 
TTGCATAGCA TTATCTCTGA GGTAGATGCA GAGAAATCGA GAGTCAATGG GATTCATGAT 
GATGATGATG ATGATGACAT CGCCTGTTCC ATTTTGCCAA GAGGCTCACA AGACACTTTG 
TTAACTAACG GTCATGTACA CCCTGTGAAT GGTGCCTTAA AGCCTCAGGA CTTTGCAGAC 
CATCATCCTC ATGTTGTTGA CAAAGCGTCC AATCAGTCTC ACCTCCCACA GGACAAATAC 
AGGGAGGGTG AGGAGCCCTG CTGCTCATAT ACTGAATCAA AAGCTAATTC CCATCTGAGG 
TCCTTAATAT CAGCAGCCAG TCCTGCAAAG CAGCCTGGTC CTCACAATAT GCAAACGGAC 
CTCCATCAAA AACCAACATC CAATTCAGGA ACTTTAGGTG CTCCTCAGAA GGCCAGTCTG 
CATGATCAGA CCACAGTCCG GCTAAATGGG AGTTATCACG TGACCTCAAA CTCCTCCACT 
CTGGCAAACT CTAGTAACCA GTCAGGAAAA TCCTCTGCAG AGGCTGGAAT AATATCCGAG 
TTCTTTTCTC ACTCAAGGCT GCATCACATT TCTACGTGGC GCAATGAGTT CTCGGAGTAT 
GTAAACAGCC TGCAGAGCAG ACGACGAGCA GCAGGTGGAG CTGTTTTCTC TGGAAGAGAG 
AAGCTGAAGA AGCTCAAAGC TAACTGCAAT TCAGGTTCCT TGATGGCTGC TCCTCAGGTG 
CGTCAGTCAT GTGTTCTCCA TGTTGATATG GACTGCTTCT TTGTCTCTGT GGGGATTAGA 
CACCGACCAG ATCTTATAGG GAAACCAGTT GCGGTGACTA GTAATCGTGG TCCAGGCCGT 
GTTGCTCAGC GCCCTGGTGC AAACCCTCAG CTTGAGTTTC AGTATTACCA GAATAAACAG 
AAACATTACA GGAAAGAGAA GACAGATGGG GATCTTGAGA TGACCCCATC TCCACAGGAT 
GGTGAAGTTC CCAGTAATGG AGTGCATGAA GACCTGGCTG CCCTTTCTAT GGCAGAGATT 
GCCTCTTGTA GTTACGAGGC CAGGCAGGCT GGTGTGCGAA ATGGCATGTT CTTTGGTAGG 
GCCAAGCAGC TGTGTCCAGA TCTGCAGTCT GTTCCATATG ACTTCCATGC TTATAAAGAG 
GTGGCTCTGG CCATGTATGA GATCCTGGCC AGTTACACTC ATAACATTGA GGCGCTGAGC 
TGTGACGAGG CACTGGTTGA TGCCACAGCT CTTCTGGTTG AGCTGGGGGT GTCTCCTGAT 
GAACTGGCAC GGTCCATCCG TGAAGACATA AAGGAGAAGA CTGGCTGCTG TGCCTCAGTT 
GGAATGAGTT CCAACATCCT ACTTGCTCGG ATGGCAACCC GTAAGGCTAA ACCCAAAGGG 
CAGTATTTAC TCAGATCAGA GGAAGTGGAT GATTTTATTA GAGACCAGCC TGTGTCCAGT 
TTACCTGGTG TGGGTCGCTC AATGAGCTCC AAGTTGACCT CTCTAGGAGT GAGTACCTGC 
GGTGACCTGC AGCAGTTGTC CCTGTCTCAA CTGCAGAGGG AGTTTGGGCC ACGGACGGGA 
CAGACGCTCT TTCGCTTTTG CAGGGGACTA GATGACAGAC CTGTGCGCAG TGAGAAAGAG 
AGAAAGTCTG TGTCTGCGGA GATGAACTAC AACATTCGGT TCACACAGGT TGAGGAGGCG 
GAGTCTTTCC TCAATAATCT CTCTATGGAG GTACAGAAGA GGTTAGAGGG GGCGGGGCTT 
AGAGGTCGTC GGGTTACACT GAAGGTCATG ATGCGGAAAC CAGGTGCGCC GGTAGAACCA 
GCCAAATATG GTGGTCATGG GATATGTGAC AACTTTGCCA GGTCTGTTTT ACTAGCTCAG 
CCCACAGACA GTGGTCGAGT GATTGCATCA GAGGCCATTA AGCTGTTTCA TGCCATGAAG 
CTGAATGTGA AGGACATGAG GGGAGTGGGG CTGCAGGTGC AGCAGCTGGA TGGATCACAT 
GCAGACCCTT CAGGACAAGG GCCCTCTCGT GGCCGATCAA TCAGAGACCT GCTTCTGGCC 
AAACAGTCTG CCCACAGCCC CAGCAAGGAG TCTCCATCAC AAGATGGTTT AATCAGCGCT 
TCGTCATCCA GAGCTTTCTC CTCCAATCAG AAGAGAGTTA CCCCTGCATT ATCACCGCCA 
CCTTCTACAT CATCATCATC ATCACCACCA CCCTCCTTCA CCATTATAGA TCCAATGCCT 
GGAACAAGTA AAGGAGATTA TCACCATCCA CACACCCCCA ACCACGTCAG GGCCTGCCTA 
AACATCAGCA TCGAGGTGCC ATCTCCCTCT CAGGTGGACC CATCTGTTTT AAAAGCTTTA 
CCTATAGACA TAAGGAGACA AGTAGAGGAA ACCTGGAGAT ACAGAGAGGA GCAGCCCAGC 
ACTTCTTCTC ATTACTCAAC ACCACCTCGA CCACCCTCCA CTCCACCTCC AGGACCTTCA 
CTTGTTCTGC AGCTCCCAAA CCAGCCTGGT CAACCATGCA CAACGGGCAT TATATTAGAG 
CTCCCGGACT TCTCCCAGGT TGACCCAGAT GTGTTTGCAG CACTCCCTAG AGAGCTTCAG 
GAAGAGTTGT GTTCTGCTTA CAGAAATAAG GGAAATGCTC AAGCTCAAGC CAGCACTGTA 
GTGGAACAAA AAAACTCATT TCCTCAACTG AAGCAGCCTG CTGTAGGGAA ATTAAAGCGC 
CGCTACAAAA AAAGAAACAC TAGTCCTGCC AAAAATGGTT CAAGTCCACT AAAAAAAATG 
TTCCCTGGGA ACAGCCCTGC CAAAACCAGC CCCTCCAAAA CTCTTCCTCT GCCACTCAGA 
GAACAAGACC TCAAATGCCC GTCCTCTCTG AGCACAGATG TCCCAGAGAC ACTACCCAAA 
CCCATTCCTC GGCCAGCCCC AACCCTTGCA GGAGCGCATG AATTCAGTGA GATAAGAACG 
CTGCTGCGTG AATGGGTCAC CACCATCTCA GAACCAATGG AGGAGGACAT TTTACAGGTG 
GTGAAGTACT GCACAGAACT AGTGGAGGAC AAAGACCTGG AGAAACTCTA CCTTGTCATC 
AAGTACATGA AGAGGCTCAT GCAGCAGTCC TCAGAGTCAG TGTGGAGCAT GGCCTTTGAC 
TTTGTCCTGG ACAATGTGCA GGTGGTGGTA CAGCAAACCT ACGGCAGCAC TTTGAAGATC 
ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of target genes downstream of semaphorin6A/PlexinA2 signaling in zebrafish.
Developmental dynamics : an official publication of the American Association of Anatomists246(7)539-549(2017 Jul)
Emerson SE,St Clair RM,Waldron AL,Bruno SR,Duong A,Driscoll HE,Ballif BA,McFarlane S,Ebert AM

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Nature cell biology15(12)1516-25(2013 Dec)
Huang HT,Kathrein KL,Barton A,Gitlin Z,Huang YH,Ward TP,Hofmann O,Dibiase A,Song A,Tyekucheva S,Hide W,Zhou Y,Zon LI

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REV1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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