tnksb cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tnksb
Entrez Gene ID	567533
Full Name	tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase b
Synonyms	si:ch211-155m12.3,wu:fe02c12
General protein information	Preferred Names tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase b Names tankyrase 1 fe02c12 tnks1b
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005171743.3	XP_005171800.1	tankyrase 1 isoform X1
XM_005171745.3	XP_005171802.1	tankyrase 1 isoform X3
XM_005171744.3	XP_005171801.1	tankyrase 1 isoform X2
XM_005171744.4	XP_005171801.1	tankyrase 1 isoform X2
XM_005171745.4	XP_005171802.1	tankyrase 1 isoform X3
XM_005171743.4	XP_005171800.1	tankyrase 1 isoform X1
NM_001089415.2	NP_001082884.1	tankyrase 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Tankyrase inhibition aggravates kidney injury in the absence of CD2AP.
Kuusela S, Wang H, Wasik AA, Suleiman H, Lehtonen S
Cell death & disease7e2302(2016 Jul)

Tankyrases regulate glucoregulatory mechanisms and somatic growth via the central melanocortin system in zebrafish larvae.
Wang H, Semenova S, Kuusela S, Panula P, Lehtonen S
FASEB journal : official publication of the Federation of American Societies for Experimental Biology29(11)4435-48(2015 Nov)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

RNA-Seq analysis in mutant zebrafish reveals role of U1C protein in alternative splicing regulation.
R?sel TD, Hung LH, Medenbach J, Donde K, Starke S, Benes V, R?tsch G, Bindereif A
The EMBO journal30(10)1965-76(2011 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Tank1/Tank2 inhibition aggravates kidney injury in the absence of CD2AP.
Title: Tankyrase inhibition aggravates kidney injury in the absence of CD2AP.

The following tnksb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tnksb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa32521	NM_001089415.2 Latest version!	Danio rerio tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase b (tnksb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa10083	XM_005171743.4 Latest version!	Danio rerio tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase b (tnksb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa10084	XM_005171744.4 Latest version!	Danio rerio tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase b (tnksb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa10085	XM_005171745.4 Latest version!	Danio rerio tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase b (tnksb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
ODa10084	XM_005171744.3	Danio rerio tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase b (tnksb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa10085	XM_005171745.3	Danio rerio tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase b (tnksb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa10083	XM_005171743.3	Danio rerio tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase b (tnksb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tnksb ( NM_001089415.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001082884.1 Danio rerio tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase b (tnksb), mRNA.

Price & Availability↑

CloneID	ODa32521
Clone ID Related Accession (Same CDS sequence)	NM_001089415.2
Accession Version	NM_001089415.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3804bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	tankyrase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC162268.1. On Jun 22, 2008 this sequence version replaced NM_001089415.1. ##Evidence-Data-START## Transcript exon combination :: BC162262.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGTGT CCCGTCGATC CTCACAGCAC CAACAAAGCA GCCTACAACC GTCTCCGCGA 
AACGCCGTAA TCGCTTCCCC TCCTCCTGAA TCGCCGTCCG CTATTATTCC CGATTCCGCG 
AGTTTATTGA GCCCTTCAGC CACTGGCGCA GCAGAATGTG GCGCATCGAC GATGGAGTCC 
GCAGCGGCCT CCTCCGCCTC CGCCTCTTCC CCAGACCGGC CCGCCCCGGT TACAGCAGCG 
GCCGCGGGGG GACCGTCAGT CCTCGGCAGC TCCAGCTGTA GCAGCATTAG CTCCAGCGCG 
ACCGCGGGCA GCCAGAGCCC CGGATCCGGA GCCGCCAGTC CCGGGGAGGC GGTGTGCGGG 
GCTGGCGGGG CGTTCAGGGA GCTGTTCGAG GCCTGTCGGA ATGGAGATGT GTCGCGGGTG 
AAGAGGCTGG TGGACTCGGT GAATGTGAAC GCTAAAGACA TGGCCGGACG AAAATCAACC 
CCTCTGCATT TCGCTGCAGG CTTCGGCAGA AAGGATGTTG TCGAGCATCT CCTGCAAACC 
GGAGCCAACG TTCATTCCCG TGATGATGGT GGTCTGATCC CGCTCCATAA CGCTTGCTCA 
TTCGGCCACG CAGAAGTGGT CAGCCTGCTG TTGTGTAGCG GCGCCGACCC CAACGCTCGA 
GACAACTGGA ACTACACACC TCTGCATGAG GCCGCCATCA AAGGCAAAAT AGACGTCTGC 
ATCGTGTTGC TTCAGCACGG TGCTGATCCA AATATCAGAA ACACTGACGG CAAATCAGCT 
CTGGATCTGG CAGACCCTTC TGCTAAGACC GTCCTCACTG GTGAATATAA GAAGGATGAA 
CTATTGGAAG CAGCGAGGAG CGGAAATGAG GAAAAACTGA TGGCACTTTT GACTCCGCTT 
AACGTCAACT GTCATGCCAG TGATGGACGC AAGTCCACTC CTCTCCACCT GGCCGCTGGG 
TATAATCGTG TGCGTATCGT GCAGTTATTG CTGCAGTACG GTGCTGATGT CCATGCCAAA 
GACAAAGGTG GTTTGGTTCC TCTGCATAAT GCCTGTTCTT ATGGTCACTA TGAGGTCACA 
GAACTCCTGC TGAAGCATGG GGCGTGTGTT AATGCCATGG ACCTCTGGCA GTTCACGCCA 
CTGCACGAGG CGGCCAGTAA GAATCGAGTG GAGGTTTGTT CACTGCTGCT GAGTCACGGC 
GCAGATCCAA CACTGCTGAA CTGCCATGGC AAGAGTGCGG TGGATGTGGC TCCCACACCT 
GAGCTGAAAG AGAGACTCAC CTATGAATTT AAAGGACACT CGCTTCTGCA AGCGGCCCGT 
GAGGCGGACA TGGCGAAGGT CAAGAAAACA GCTCAAGAAA TCATCAGCTT CAAACACCCG 
CACTCGCACG ACTCTGCTCT GCACTGCGCC GTAGCCTCTC CTCATCCCAA ACGCAAACAG 
GTCACAGAGT TACTGCTGAG GAAAGGTGCC AACATTCACG AGAAGAACAA GGATTTCATG 
ACCCCGTTTC ACGTAGCAGC TGAACGAGGT CATAATGATG TGCTTGAAGT CCTGCAGAAA 
CACGGAGCAA AGGTGAACGC TGCGGACACA CTGGGTCAGA CGGCTCTGCA CAGGGCGGCT 
CTTGCTGGTC ACATCCAGAC CTGCAGATTA CTGCTGAGTT ACGGAGCAGA TCCTTCAATC 
GTCTCACTGC AGGGCTTTAC TGCATCACAG ATGGGGAATG AAGCCGTTCA GCAAATACTC 
AATGAGAACG TTCCTCCCCG TAATTCAGAT GTGGATTACC GCTTGCTCGA AGCTGCTAAA 
GCTGGCGACC TTGATACAGT GAAGCAACTG TGCTCTCCTC AGAACGTGAA CTGTCGGGAT 
CTGGAGGGCC GTCACTCTAC ACCGCTACAC TTCGCCGCAG GCTACAACCG GGTGGCTGTG 
GTGGAGTATC TGCTCCATCA CGGGGCCGAT GTGCACGCTA AAGATAAAGG GGGTCTGGTT 
CCTCTGCACA ACGCATGCTC GTATGGTCAC TACGAGGTGG CGGAGCTGCT GGTGAGACAT 
GGAGCATCTG TGAACGTGGC CGATCTCTGG AAGTTCACAC CACTACATGA GGCTGCAGCA 
AAGGGCAAAT ACGAGATCTG CAAACTACTG CTCAAGCATG GTGCAGACCC GTCTAAGAAG 
AACCGTGATG GTAACATGGC TCTGGACATG GTGAAAGATG GAGACACGGA CATTCAAGAC 
CTGCTGCGTG GAGACGCCGC TCTGCTGGAC GCTGCCAAAA AGGGCTGTTT GGCCCGAGTG 
CAGAAACTCT GCAGCCCGGA GAACATCAAC TGCAGAGACA CACAGGGCAG GAACTCCACA 
CCGCTGCACC TTGCGGCTGG CTATAATAAC TTGGAGGTGG CCGAGTATCT CCTGGAGCAT 
GGAGCGGATG TGAACGCTCA GGATAAAGGA GGACTGATTC CTCTGCATAA TGCTGCTTCA 
TATGGGCATG TGGACATCGC CGCCCTGCTG ATCAAGTTCA ACACGTGTGT AAATGCGACA 
GATAAGTGGG CCTTCACTCC TTTGCATGAA GCTGCTCAGA AGGGCCGAAC ACAGCTGTGT 
GCACTGCTAC TGGCACATGG TGCAAACCCC ACCATGAAGA ATCAAGAGGG ACAGACTGCT 
CTGGACCTCG CCACGGCTGA TGACATCCGT GCTCTGCTGA TGGATGCGAT GCCCCCTGAT 
GCCCTGCCTA GCTGTTTCAA ACCGCAGGCC ACCGTGCTCA GCGCCGCGCT CATTTCTCCC 
GCCTCCACAC CCTCCTGCCT CTCCGCCGCC TCCAGCATCG ACAACCTGGC TGGACCTCTA 
TGTGACGGAG CCAGTGGAGG AGCCGCGGGA CCCGCTGATG GAGCCTCCAG CACTGATCGC 
AAAGAAGGAG ATGCTGTCCT GGATATGAAC ATCTCCCAGT TCCTGAAGAG TTTAGGCCTT 
GAACATTTGA GGGACATCTT TGAGAGAGAA CAGATCACCC TGGACGTATT GGCTGATATG 
GGTCATGAGG AACTCAAGGA GATAGGAATC AACGCTTACG GACACCGTCA TAAACTCATT 
AAAGGCATTG AGAGGCTGCT GGGAGGACAG CAGGGTGCCA ATCCATACCT GACGTTCCAC 
TGCACCAATC AGGGCACAGT ATTGATTGAC CTGGCTGTGG ATGATAAGGA GTTTCAGTCC 
GTTGAGGAAG AGATGCAAAG CACAATTCGG GAACACAGGG ATGGCGGAAA TGCAGGTGGC 
GTTTTCAGTC GTTACAATGT CATTAAGATC CAGAAGGTGG TGAATAAGAA ACTGAGAGAG 
CGATACACAC ACAGGCAGAA AGAGATCGCG GACGAGAATC ACAATCACCA CAATGAGCGA 
ATGCTGTTTC ACGGATCTCC GTTTATAAAC GCCATCATCC ATAAAGGCTT TGATGAGCGT 
CATGCGTATA TCGGCGGCAT GTTTGGAGCG GGTATATATT TTGCAGAGAA CTCTTCAAAG 
AGTAACCAGT ACGTCTACGG CATCGGTGGT GGTACAGGAT GCCCAACTCA CAAGGATCGT 
TCCTGTTACC TGTGCCACAG GCAAATGTTG TTCTGCCGTG TGACATTGGG AAAATCCTTC 
TTGCAGTTCA GTGCCATGAA GATGGCCCAC GCTCCGCCTG GGCACCACTC TGTGATCGGC 
CGTCCCAGTG TCAACGGCCT GGCCTACGCA GAGTATGTGA TCTATCGCGG AGAACAGGCC 
TACCCAGAGT ATCTCATCAC CTATCAGATA ATGAAGCCGG ACAGCACGCC TCAGTCCTCC 
ACAGGAGCGG AGCAGAAATC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001082884.1
CDS	47..3850
Translation	MAVSRRSSQHQQSSLQPSPRNAVIASPPPESPSAIIPDSASLLSPSATGAAECGASTMESAAASSASASSPDRPAPVTAAAAGGPSVLGSSSCSSISSSATAGSQSPGSGAASPGEAVCGAGGAFRELFEACRNGDVSRVKRLVDSVNVNAKDMAGRKSTPLHFAAGFGRKDVVEHLLQTGANVHSRDDGGLIPLHNACSFGHAEVVSLLLCSGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLADPSAKTVLTGEYKKDELLEAARSGNEEKLMALLTPLNVNCHASDGRKSTPLHLAAGYNRVRIVQLLLQYGADVHAKDKGGLVPLHNACSYGHYEVTELLLKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSHGADPTLLNCHGKSAVDVAPTPELKERLTYEFKGHSLLQAAREADMAKVKKTAQEIISFKHPHSHDSALHCAVASPHPKRKQVTELLLRKGANIHEKNKDFMTPFHVAAERGHNDVLEVLQKHGAKVNAADTLGQTALHRAALAGHIQTCRLLLSYGADPSIVSLQGFTASQMGNEAVQQILNENVPPRNSDVDYRLLEAAKAGDLDTVKQLCSPQNVNCRDLEGRHSTPLHFAAGYNRVAVVEYLLHHGADVHAKDKGGLVPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKGKYEICKLLLKHGADPSKKNRDGNMALDMVKDGDTDIQDLLRGDAALLDAAKKGCLARVQKLCSPENINCRDTQGRNSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYGHVDIAALLIKFNTCVNATDKWAFTPLHEAAQKGRTQLCALLLAHGANPTMKNQEGQTALDLATADDIRALLMDAMPPDALPSCFKPQATVLSAALISPASTPSCLSAASSIDNLAGPLCDGASGGAAGPADGASSTDRKEGDAVLDMNISQFLKSLGLEHLRDIFEREQITLDVLADMGHEELKEIGINAYGHRHKLIKGIERLLGGQQGANPYLTFHCTNQGTVLIDLAVDDKEFQSVEEEMQSTIREHRDGGNAGGVFSRYNVIKIQKVVNKKLRERYTHRQKEIADENHNHHNERMLFHGSPFINAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPTHKDRSCYLCHRQMLFCRVTLGKSFLQFSAMKMAHAPPGHHSVIGRPSVNGLAYAEYVIYRGEQAYPEYLITYQIMKPDSTPQSSTGAEQKS

Target ORF information:

RefSeq Version	NM_001089415.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase b (tnksb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001089415.2

ORF Insert Sequence:

ATGGCGGTGT CCCGTCGATC CTCACAGCAC CAACAAAGCA GCCTACAACC GTCTCCGCGA 
AACGCCGTAA TCGCTTCCCC TCCTCCTGAA TCGCCGTCCG CTATTATTCC CGATTCCGCG 
AGTTTATTGA GCCCTTCAGC CACTGGCGCA GCAGAATGTG GCGCATCGAC GATGGAGTCC 
GCAGCGGCCT CCTCCGCCTC CGCCTCTTCC CCAGACCGGC CCGCCCCGGT TACAGCAGCG 
GCCGCGGGGG GACCGTCAGT CCTCGGCAGC TCCAGCTGTA GCAGCATTAG CTCCAGCGCG 
ACCGCGGGCA GCCAGAGCCC CGGATCCGGA GCCGCCAGTC CCGGGGAGGC GGTGTGCGGG 
GCTGGCGGGG CGTTCAGGGA GCTGTTCGAG GCCTGTCGGA ATGGAGATGT GTCGCGGGTG 
AAGAGGCTGG TGGACTCGGT GAATGTGAAC GCTAAAGACA TGGCCGGACG AAAATCAACC 
CCTCTGCATT TCGCTGCAGG CTTCGGCAGA AAGGATGTTG TCGAGCATCT CCTGCAAACC 
GGAGCCAACG TTCATTCCCG TGATGATGGT GGTCTGATCC CGCTCCATAA CGCTTGCTCA 
TTCGGCCACG CAGAAGTGGT CAGCCTGCTG TTGTGTAGCG GCGCCGACCC CAACGCTCGA 
GACAACTGGA ACTACACACC TCTGCATGAG GCCGCCATCA AAGGCAAAAT AGACGTCTGC 
ATCGTGTTGC TTCAGCACGG TGCTGATCCA AATATCAGAA ACACTGACGG CAAATCAGCT 
CTGGATCTGG CAGACCCTTC TGCTAAGACC GTCCTCACTG GTGAATATAA GAAGGATGAA 
CTATTGGAAG CAGCGAGGAG CGGAAATGAG GAAAAACTGA TGGCACTTTT GACTCCGCTT 
AACGTCAACT GTCATGCCAG TGATGGACGC AAGTCCACTC CTCTCCACCT GGCCGCTGGG 
TATAATCGTG TGCGTATCGT GCAGTTATTG CTGCAGTACG GTGCTGATGT CCATGCCAAA 
GACAAAGGTG GTTTGGTTCC TCTGCATAAT GCCTGTTCTT ATGGTCACTA TGAGGTCACA 
GAACTCCTGC TGAAGCATGG GGCGTGTGTT AATGCCATGG ACCTCTGGCA GTTCACGCCA 
CTGCACGAGG CGGCCAGTAA GAATCGAGTG GAGGTTTGTT CACTGCTGCT GAGTCACGGC 
GCAGATCCAA CACTGCTGAA CTGCCATGGC AAGAGTGCGG TGGATGTGGC TCCCACACCT 
GAGCTGAAAG AGAGACTCAC CTATGAATTT AAAGGACACT CGCTTCTGCA AGCGGCCCGT 
GAGGCGGACA TGGCGAAGGT CAAGAAAACA GCTCAAGAAA TCATCAGCTT CAAACACCCG 
CACTCGCACG ACTCTGCTCT GCACTGCGCC GTAGCCTCTC CTCATCCCAA ACGCAAACAG 
GTCACAGAGT TACTGCTGAG GAAAGGTGCC AACATTCACG AGAAGAACAA GGATTTCATG 
ACCCCGTTTC ACGTAGCAGC TGAACGAGGT CATAATGATG TGCTTGAAGT CCTGCAGAAA 
CACGGAGCAA AGGTGAACGC TGCGGACACA CTGGGTCAGA CGGCTCTGCA CAGGGCGGCT 
CTTGCTGGTC ACATCCAGAC CTGCAGATTA CTGCTGAGTT ACGGAGCAGA TCCTTCAATC 
GTCTCACTGC AGGGCTTTAC TGCATCACAG ATGGGGAATG AAGCCGTTCA GCAAATACTC 
AATGAGAACG TTCCTCCCCG TAATTCAGAT GTGGATTACC GCTTGCTCGA AGCTGCTAAA 
GCTGGCGACC TTGATACAGT GAAGCAACTG TGCTCTCCTC AGAACGTGAA CTGTCGGGAT 
CTGGAGGGCC GTCACTCTAC ACCGCTACAC TTCGCCGCAG GCTACAACCG GGTGGCTGTG 
GTGGAGTATC TGCTCCATCA CGGGGCCGAT GTGCACGCTA AAGATAAAGG GGGTCTGGTT 
CCTCTGCACA ACGCATGCTC GTATGGTCAC TACGAGGTGG CGGAGCTGCT GGTGAGACAT 
GGAGCATCTG TGAACGTGGC CGATCTCTGG AAGTTCACAC CACTACATGA GGCTGCAGCA 
AAGGGCAAAT ACGAGATCTG CAAACTACTG CTCAAGCATG GTGCAGACCC GTCTAAGAAG 
AACCGTGATG GTAACATGGC TCTGGACATG GTGAAAGATG GAGACACGGA CATTCAAGAC 
CTGCTGCGTG GAGACGCCGC TCTGCTGGAC GCTGCCAAAA AGGGCTGTTT GGCCCGAGTG 
CAGAAACTCT GCAGCCCGGA GAACATCAAC TGCAGAGACA CACAGGGCAG GAACTCCACA 
CCGCTGCACC TTGCGGCTGG CTATAATAAC TTGGAGGTGG CCGAGTATCT CCTGGAGCAT 
GGAGCGGATG TGAACGCTCA GGATAAAGGA GGACTGATTC CTCTGCATAA TGCTGCTTCA 
TATGGGCATG TGGACATCGC CGCCCTGCTG ATCAAGTTCA ACACGTGTGT AAATGCGACA 
GATAAGTGGG CCTTCACTCC TTTGCATGAA GCTGCTCAGA AGGGCCGAAC ACAGCTGTGT 
GCACTGCTAC TGGCACATGG TGCAAACCCC ACCATGAAGA ATCAAGAGGG ACAGACTGCT 
CTGGACCTCG CCACGGCTGA TGACATCCGT GCTCTGCTGA TGGATGCGAT GCCCCCTGAT 
GCCCTGCCTA GCTGTTTCAA ACCGCAGGCC ACCGTGCTCA GCGCCGCGCT CATTTCTCCC 
GCCTCCACAC CCTCCTGCCT CTCCGCCGCC TCCAGCATCG ACAACCTGGC TGGACCTCTA 
TGTGACGGAG CCAGTGGAGG AGCCGCGGGA CCCGCTGATG GAGCCTCCAG CACTGATCGC 
AAAGAAGGAG ATGCTGTCCT GGATATGAAC ATCTCCCAGT TCCTGAAGAG TTTAGGCCTT 
GAACATTTGA GGGACATCTT TGAGAGAGAA CAGATCACCC TGGACGTATT GGCTGATATG 
GGTCATGAGG AACTCAAGGA GATAGGAATC AACGCTTACG GACACCGTCA TAAACTCATT 
AAAGGCATTG AGAGGCTGCT GGGAGGACAG CAGGGTGCCA ATCCATACCT GACGTTCCAC 
TGCACCAATC AGGGCACAGT ATTGATTGAC CTGGCTGTGG ATGATAAGGA GTTTCAGTCC 
GTTGAGGAAG AGATGCAAAG CACAATTCGG GAACACAGGG ATGGCGGAAA TGCAGGTGGC 
GTTTTCAGTC GTTACAATGT CATTAAGATC CAGAAGGTGG TGAATAAGAA ACTGAGAGAG 
CGATACACAC ACAGGCAGAA AGAGATCGCG GACGAGAATC ACAATCACCA CAATGAGCGA 
ATGCTGTTTC ACGGATCTCC GTTTATAAAC GCCATCATCC ATAAAGGCTT TGATGAGCGT 
CATGCGTATA TCGGCGGCAT GTTTGGAGCG GGTATATATT TTGCAGAGAA CTCTTCAAAG 
AGTAACCAGT ACGTCTACGG CATCGGTGGT GGTACAGGAT GCCCAACTCA CAAGGATCGT 
TCCTGTTACC TGTGCCACAG GCAAATGTTG TTCTGCCGTG TGACATTGGG AAAATCCTTC 
TTGCAGTTCA GTGCCATGAA GATGGCCCAC GCTCCGCCTG GGCACCACTC TGTGATCGGC 
CGTCCCAGTG TCAACGGCCT GGCCTACGCA GAGTATGTGA TCTATCGCGG AGAACAGGCC 
TACCCAGAGT ATCTCATCAC CTATCAGATA ATGAAGCCGG ACAGCACGCC TCAGTCCTCC 
ACAGGAGCGG AGCAGAAATC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnksb ( XM_005171743.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171800.1 Danio rerio tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase b (tnksb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa10083
Clone ID Related Accession (Same CDS sequence)	XM_005171743.3 , XM_005171743.4
Accession Version	XM_005171743.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tankyrase 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005171743.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGT CCCGTCGATC CTCACAGCAC CAACAAAGCA GCCTACAACC GTCTCCGCGA 
AACGCCGTAA TCGCTTCCCC TCCTCCTGAA TCGCCGTCCG CTATTATTCC CGATTCCGCG 
AGTTTATTGA GCCCTTCAGC CACTGGCGCA GCAGAATGTG GCGCATCGAC GATGGAGTCC 
GCAGCGGCCT CCTCCGCCTC CGCCTCTTCC CCAGACCGGC CCGCCCCGGT AACAGCAGCG 
GCCGCGGGGG GACCGTCAGT CCTCGGCAGC TCCAGCTGTA GCAGCATTAG CTCCAGCGCG 
ACCGCGGGCA GCCAGAGCCC CGGATCCGGA GCCGCCAGTC CCGGGGAGGC GGTGTGCGGG 
GCTGGCGGGG CGTTCAGGGA GCTGTTCGAG GCCTGTCGGA ATGGAGATGT GTCGCGGGTG 
AAGAGGCTGG TGGACTCGGT GAATGTGAAC GCTAAAGACA TGGCCGGACG AAAATCAACC 
CCTCTGCATT TCGCTGCAGG CTTCGGCAGA AAGGATGTTG TCGAGCATCT CCTGCAAACC 
GGAGCCAACG TTCATTCCCG TGATGATGGT GGTCTGATCC CGCTCCATAA CGCTTGCTCA 
TTCGGCCACG CAGAAGTGGT CAGCCTGCTG TTGTGTAGCG GCGCCGACCC CAACGCTCGA 
GACAACTGGA ACTACACACC TCTGCATGAG GCCGCCATCA AAGGCAAAAT AGACGTCTGC 
ATCGTGTTGC TTCAGCACGG TGCTGATCCA AATATCAGAA ACACTGACGG CAAATCAGCT 
CTGGATCTGG CAGACCCTTC TGCTAAGACC GTCCTCACTG GTGAATATAA GAAGGATGAA 
CTATTGGAAG CAGCGAGGAG CGGAAATGAG GAAAAACTGA TGGCACTTTT GACCCCGCTT 
AACGTCAACT GTCATGCCAG TGATGGACGC AAGTCAACAT CACAAAAAAT GCTGTCCACT 
CCTCTCCACC TGGCCGCTGG GTATAATCGT GTGCGTATCG TGCAGTTATT GCTGCAGTAC 
GGTGCAGATG TCCATGCCAA AGACAAAGGT GGTTTGGTTC CTCTGCATAA TGCCTGTTCT 
TATGGTCACT ATGAGGTCAC AGAACTCCTG CTGAAGCATG GGGCGTGTGT TAATGCCATG 
GACCTCTGGC AGTTCACGCC GCTGCACGAG GCGGCCAGTA AGAATCGAGT GGAGGTTTGT 
TCTCTGCTGC TGAGTCACGG CGCAGATCCA ACACTGCTGA ACTGCCACGG CAAGAGTGCG 
GTGGATGTGG CTCCCACACC TGAGCTGAAA GAGAGACTCA CCTATGAATT TAAAGGACAC 
TCGCTTCTGC AAGCGGCCCG TGAGGCGGAC ATGGCGAAGG TCAAGAAAAC AGCTCAAGAA 
ATCATCAGCT TCAAACACCC GCACTCGCAC GACTCCGCTC TGCACTGCGC CGTAGCCTCT 
CCTCATCCCA AACGCAAACA GGTCACAGAG TTACTGCTGA GGAAAGGTGC CAACATTCAC 
GAGAAGAACA AGGATTTCAT GACCCCGTTT CACGTAGCAG CTGAACGAGG TCATAATGAT 
GTGCTTGAAG TCCTGCAGAA ACACGGAGCA AAGGTGAACG CTGCGGACAC ACTGGGTCAG 
ACGGCTCTGC ACAGGGCTGC TCTTGCTGGT CACATCCAGA CCTGCAGATT ACTGCTGAGT 
TACGGAGCAG ATCCTTCAAT CGTCTCACTG CAGGGCTTCA CTGCATCACA GATGGGGAAT 
GAAGCCGTTC AGCAAATACT CAATGAGAAC GTTCCTCCCC GTAATTCAGA TGTGGATTAC 
CGCTTGCTCG AAGCTGCTAA AGCTGGCGAC CTTGATACAG TGAAGCAACT GTGCTCTCCT 
CAGAACGTGA ACTGTCGGGA TCTGGAGGGC CGTCACTCTA CACCGCTACA CTTCGCCGCA 
GGCTACAACC GGGTGGCTGT GGTGGAGTAT CTGCTCCATC ACGGGGCCGA TGTGCACGCT 
AAAGATAAAG GGGGTCTGGT TCCTCTGCAC AACGCATGCT CGTATGGTCA CTACGAGGTG 
GCGGAGCTGC TGGTGAGACA TGGAGCATCT GTGAACGTGG CCGATCTCTG GAAGTTCACA 
CCACTACATG AGGCTGCAGC AAAGGGCAAA TACGAGATCT GCAAACTACT GCTCAAGCAT 
GGTGCAGACC CGTCTAAGAA GAACCGTGAT GGTAACATGG CTCTGGACAT GGTGAAAGAT 
GGAGACACGG ACATTCAAGA CCTGCTGCGT GGAGACGCCG CTCTGCTGGA CGCTGCCAAG 
AAGGGCTGTT TGGCCCGAGT GCAGAAACTC TGCAGCCCGG AGAACATCAA CTGCAGAGAC 
ACACAGGGCA GGAACTCCAC ACCGCTGCAC CTTGCGGCTG GCTATAATAA CTTGGAGGTG 
GCCGAGTATC TCCTGGAGCA TGGAGCGGAT GTGAACGCTC AGGATAAAGG AGGACTGATT 
CCTCTGCATA ATGCTGCTTC ATATGGGCAT GTGGACATCG CCGCCCTGCT GATCAAGTTC 
AACACGTGTG TAAATGCGAC AGATAAGTGG GCCTTCACTC CTTTGCATGA AGCTGCTCAG 
AAGGGCCGAA CACAGCTGTG TGCACTGCTA CTGGCACATG GTGCAAACCC CACCATGAAG 
AATCAAGAGG GACAGACTGC TCTGGACCTC GCCACGGCTG ATGACATCCG TGCTCTGCTG 
ATGGATGCGA TGCCCCCTGA TGCCCTGCCT AGCTGTTTCA AACCGCAGGC CACCGTGCTC 
AGCGCCGCGC TCATTTCTCC CGCCTCCACA CCCTCCTGCC TCTCCGCCGC CTCCAGCATC 
GACAACCTGG CTGGACCTCT ATGTGACGGA GCCAGTGGAG GAGCCGCGGG ACCCGCTGAT 
GGAGCCTCCA GCACTGATCG CAAAGAAGGA GATGGTGCGT TAATACAAGG AAACAAACTA 
ACTGCTGTCC TGGATATGAA CATCTCCCAG TTCCTGAAGA GTTTAGGCCT TGAACATTTG 
AGGGACATCT TTGAGAGAGA ACAGATCACC CTGGACGTAT TGGCTGATAT GGGTCATGAG 
GAACTCAAGG AGATAGGAAT CAACGCTTAC GGACACCGTC ATAAACTCAT TAAAGGCATT 
GAGAGGCTGC TGGGAGGACA GCAGGGTGCC AATCCATACC TGACGTTCCA CTGCACCAAT 
CAGGGCACAG TATTGATTGA CCTGGCTGTG GATGATAAGG AGTTTCAGTC CGTTGAGGAA 
GAGATGCAAA GCACAATTCG GGAACACAGG GATGGCGGAA ATGCAGGTGG CGTTTTCAGT 
CGTTACAATG TCATTAAGAT CCAGAAGGTG GTGAATAAGA AACTGAGAGA ACGATACACA 
CACAGGCAGA AAGAGATCGC GGACGAGAAT CACAATCACC ACAATGAGCG AATGCTGTTT 
CACGGATCTC CGTTTATAAA CGCCATCATC CATAAAGGCT TTGATGAGCG TCATGCGTAT 
ATCGGCGGCA TGTTTGGAGC GGGTATATAT TTTGCAGAGA ACTCTTCAAA GAGTAACCAG 
TACGTCTACG GCATCGGTGG TGGTACAGGA TGCCCAACTC ACAAGGATCG TTCCTGTTAC 
CTGTGCCACA GGCAAATGTT GTTCTGCCGT GTGACATTGG GAAAATCCTT CTTGCAGTTC 
AGTGCCATGA AGATGGCCCA CGCTCCGCCT GGGCACCACT CTGTGATCGG CCGTCCCAGT 
GTCAACGGCC TGGCCTACGC AGAGTATGTG ATCTATCGCG GAGAACAGGC CTACCCAGAG 
TATCTCATCA CCTATCAGAT AATGAAGCCG GACAGCACGC CTCAGTCCTC CACAGGAGCG 
GAGCAGAAAT CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171800.1
CDS	76..3930
Translation	MAVSRRSSQHQQSSLQPSPRNAVIASPPPESPSAIIPDSASLLSPSATGAAECGASTMESAAASSASASSPDRPAPVTAAAAGGPSVLGSSSCSSISSSATAGSQSPGSGAASPGEAVCGAGGAFRELFEACRNGDVSRVKRLVDSVNVNAKDMAGRKSTPLHFAAGFGRKDVVEHLLQTGANVHSRDDGGLIPLHNACSFGHAEVVSLLLCSGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLADPSAKTVLTGEYKKDELLEAARSGNEEKLMALLTPLNVNCHASDGRKSTSQKMLSTPLHLAAGYNRVRIVQLLLQYGADVHAKDKGGLVPLHNACSYGHYEVTELLLKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSHGADPTLLNCHGKSAVDVAPTPELKERLTYEFKGHSLLQAAREADMAKVKKTAQEIISFKHPHSHDSALHCAVASPHPKRKQVTELLLRKGANIHEKNKDFMTPFHVAAERGHNDVLEVLQKHGAKVNAADTLGQTALHRAALAGHIQTCRLLLSYGADPSIVSLQGFTASQMGNEAVQQILNENVPPRNSDVDYRLLEAAKAGDLDTVKQLCSPQNVNCRDLEGRHSTPLHFAAGYNRVAVVEYLLHHGADVHAKDKGGLVPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKGKYEICKLLLKHGADPSKKNRDGNMALDMVKDGDTDIQDLLRGDAALLDAAKKGCLARVQKLCSPENINCRDTQGRNSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYGHVDIAALLIKFNTCVNATDKWAFTPLHEAAQKGRTQLCALLLAHGANPTMKNQEGQTALDLATADDIRALLMDAMPPDALPSCFKPQATVLSAALISPASTPSCLSAASSIDNLAGPLCDGASGGAAGPADGASSTDRKEGDGALIQGNKLTAVLDMNISQFLKSLGLEHLRDIFEREQITLDVLADMGHEELKEIGINAYGHRHKLIKGIERLLGGQQGANPYLTFHCTNQGTVLIDLAVDDKEFQSVEEEMQSTIREHRDGGNAGGVFSRYNVIKIQKVVNKKLRERYTHRQKEIADENHNHHNERMLFHGSPFINAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPTHKDRSCYLCHRQMLFCRVTLGKSFLQFSAMKMAHAPPGHHSVIGRPSVNGLAYAEYVIYRGEQAYPEYLITYQIMKPDSTPQSSTGAEQKS

Target ORF information:

RefSeq Version	XM_005171743.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase b (tnksb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171743.3

ORF Insert Sequence:

ATGGCGGTGT CCCGTCGATC CTCACAGCAC CAACAAAGCA GCCTACAACC GTCTCCGCGA 
AACGCCGTAA TCGCTTCCCC TCCTCCTGAA TCGCCGTCCG CTATTATTCC CGATTCCGCG 
AGTTTATTGA GCCCTTCAGC CACTGGCGCA GCAGAATGTG GCGCATCGAC GATGGAGTCC 
GCAGCGGCCT CCTCCGCCTC CGCCTCTTCC CCAGACCGGC CCGCCCCGGT AACAGCAGCG 
GCCGCGGGGG GACCGTCAGT CCTCGGCAGC TCCAGCTGTA GCAGCATTAG CTCCAGCGCG 
ACCGCGGGCA GCCAGAGCCC CGGATCCGGA GCCGCCAGTC CCGGGGAGGC GGTGTGCGGG 
GCTGGCGGGG CGTTCAGGGA GCTGTTCGAG GCCTGTCGGA ATGGAGATGT GTCGCGGGTG 
AAGAGGCTGG TGGACTCGGT GAATGTGAAC GCTAAAGACA TGGCCGGACG AAAATCAACC 
CCTCTGCATT TCGCTGCAGG CTTCGGCAGA AAGGATGTTG TCGAGCATCT CCTGCAAACC 
GGAGCCAACG TTCATTCCCG TGATGATGGT GGTCTGATCC CGCTCCATAA CGCTTGCTCA 
TTCGGCCACG CAGAAGTGGT CAGCCTGCTG TTGTGTAGCG GCGCCGACCC CAACGCTCGA 
GACAACTGGA ACTACACACC TCTGCATGAG GCCGCCATCA AAGGCAAAAT AGACGTCTGC 
ATCGTGTTGC TTCAGCACGG TGCTGATCCA AATATCAGAA ACACTGACGG CAAATCAGCT 
CTGGATCTGG CAGACCCTTC TGCTAAGACC GTCCTCACTG GTGAATATAA GAAGGATGAA 
CTATTGGAAG CAGCGAGGAG CGGAAATGAG GAAAAACTGA TGGCACTTTT GACCCCGCTT 
AACGTCAACT GTCATGCCAG TGATGGACGC AAGTCAACAT CACAAAAAAT GCTGTCCACT 
CCTCTCCACC TGGCCGCTGG GTATAATCGT GTGCGTATCG TGCAGTTATT GCTGCAGTAC 
GGTGCAGATG TCCATGCCAA AGACAAAGGT GGTTTGGTTC CTCTGCATAA TGCCTGTTCT 
TATGGTCACT ATGAGGTCAC AGAACTCCTG CTGAAGCATG GGGCGTGTGT TAATGCCATG 
GACCTCTGGC AGTTCACGCC GCTGCACGAG GCGGCCAGTA AGAATCGAGT GGAGGTTTGT 
TCTCTGCTGC TGAGTCACGG CGCAGATCCA ACACTGCTGA ACTGCCACGG CAAGAGTGCG 
GTGGATGTGG CTCCCACACC TGAGCTGAAA GAGAGACTCA CCTATGAATT TAAAGGACAC 
TCGCTTCTGC AAGCGGCCCG TGAGGCGGAC ATGGCGAAGG TCAAGAAAAC AGCTCAAGAA 
ATCATCAGCT TCAAACACCC GCACTCGCAC GACTCCGCTC TGCACTGCGC CGTAGCCTCT 
CCTCATCCCA AACGCAAACA GGTCACAGAG TTACTGCTGA GGAAAGGTGC CAACATTCAC 
GAGAAGAACA AGGATTTCAT GACCCCGTTT CACGTAGCAG CTGAACGAGG TCATAATGAT 
GTGCTTGAAG TCCTGCAGAA ACACGGAGCA AAGGTGAACG CTGCGGACAC ACTGGGTCAG 
ACGGCTCTGC ACAGGGCTGC TCTTGCTGGT CACATCCAGA CCTGCAGATT ACTGCTGAGT 
TACGGAGCAG ATCCTTCAAT CGTCTCACTG CAGGGCTTCA CTGCATCACA GATGGGGAAT 
GAAGCCGTTC AGCAAATACT CAATGAGAAC GTTCCTCCCC GTAATTCAGA TGTGGATTAC 
CGCTTGCTCG AAGCTGCTAA AGCTGGCGAC CTTGATACAG TGAAGCAACT GTGCTCTCCT 
CAGAACGTGA ACTGTCGGGA TCTGGAGGGC CGTCACTCTA CACCGCTACA CTTCGCCGCA 
GGCTACAACC GGGTGGCTGT GGTGGAGTAT CTGCTCCATC ACGGGGCCGA TGTGCACGCT 
AAAGATAAAG GGGGTCTGGT TCCTCTGCAC AACGCATGCT CGTATGGTCA CTACGAGGTG 
GCGGAGCTGC TGGTGAGACA TGGAGCATCT GTGAACGTGG CCGATCTCTG GAAGTTCACA 
CCACTACATG AGGCTGCAGC AAAGGGCAAA TACGAGATCT GCAAACTACT GCTCAAGCAT 
GGTGCAGACC CGTCTAAGAA GAACCGTGAT GGTAACATGG CTCTGGACAT GGTGAAAGAT 
GGAGACACGG ACATTCAAGA CCTGCTGCGT GGAGACGCCG CTCTGCTGGA CGCTGCCAAG 
AAGGGCTGTT TGGCCCGAGT GCAGAAACTC TGCAGCCCGG AGAACATCAA CTGCAGAGAC 
ACACAGGGCA GGAACTCCAC ACCGCTGCAC CTTGCGGCTG GCTATAATAA CTTGGAGGTG 
GCCGAGTATC TCCTGGAGCA TGGAGCGGAT GTGAACGCTC AGGATAAAGG AGGACTGATT 
CCTCTGCATA ATGCTGCTTC ATATGGGCAT GTGGACATCG CCGCCCTGCT GATCAAGTTC 
AACACGTGTG TAAATGCGAC AGATAAGTGG GCCTTCACTC CTTTGCATGA AGCTGCTCAG 
AAGGGCCGAA CACAGCTGTG TGCACTGCTA CTGGCACATG GTGCAAACCC CACCATGAAG 
AATCAAGAGG GACAGACTGC TCTGGACCTC GCCACGGCTG ATGACATCCG TGCTCTGCTG 
ATGGATGCGA TGCCCCCTGA TGCCCTGCCT AGCTGTTTCA AACCGCAGGC CACCGTGCTC 
AGCGCCGCGC TCATTTCTCC CGCCTCCACA CCCTCCTGCC TCTCCGCCGC CTCCAGCATC 
GACAACCTGG CTGGACCTCT ATGTGACGGA GCCAGTGGAG GAGCCGCGGG ACCCGCTGAT 
GGAGCCTCCA GCACTGATCG CAAAGAAGGA GATGGTGCGT TAATACAAGG AAACAAACTA 
ACTGCTGTCC TGGATATGAA CATCTCCCAG TTCCTGAAGA GTTTAGGCCT TGAACATTTG 
AGGGACATCT TTGAGAGAGA ACAGATCACC CTGGACGTAT TGGCTGATAT GGGTCATGAG 
GAACTCAAGG AGATAGGAAT CAACGCTTAC GGACACCGTC ATAAACTCAT TAAAGGCATT 
GAGAGGCTGC TGGGAGGACA GCAGGGTGCC AATCCATACC TGACGTTCCA CTGCACCAAT 
CAGGGCACAG TATTGATTGA CCTGGCTGTG GATGATAAGG AGTTTCAGTC CGTTGAGGAA 
GAGATGCAAA GCACAATTCG GGAACACAGG GATGGCGGAA ATGCAGGTGG CGTTTTCAGT 
CGTTACAATG TCATTAAGAT CCAGAAGGTG GTGAATAAGA AACTGAGAGA ACGATACACA 
CACAGGCAGA AAGAGATCGC GGACGAGAAT CACAATCACC ACAATGAGCG AATGCTGTTT 
CACGGATCTC CGTTTATAAA CGCCATCATC CATAAAGGCT TTGATGAGCG TCATGCGTAT 
ATCGGCGGCA TGTTTGGAGC GGGTATATAT TTTGCAGAGA ACTCTTCAAA GAGTAACCAG 
TACGTCTACG GCATCGGTGG TGGTACAGGA TGCCCAACTC ACAAGGATCG TTCCTGTTAC 
CTGTGCCACA GGCAAATGTT GTTCTGCCGT GTGACATTGG GAAAATCCTT CTTGCAGTTC 
AGTGCCATGA AGATGGCCCA CGCTCCGCCT GGGCACCACT CTGTGATCGG CCGTCCCAGT 
GTCAACGGCC TGGCCTACGC AGAGTATGTG ATCTATCGCG GAGAACAGGC CTACCCAGAG 
TATCTCATCA CCTATCAGAT AATGAAGCCG GACAGCACGC CTCAGTCCTC CACAGGAGCG 
GAGCAGAAAT CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnksb ( XM_005171743.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171800.1 Danio rerio tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase b (tnksb), transcript variant X1, mRNA.

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CloneID	ODa10083
Clone ID Related Accession (Same CDS sequence)	XM_005171743.3 , XM_005171743.4
Accession Version	XM_005171743.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tankyrase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005171743.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGT CCCGTCGATC CTCACAGCAC CAACAAAGCA GCCTACAACC GTCTCCGCGA 
AACGCCGTAA TCGCTTCCCC TCCTCCTGAA TCGCCGTCCG CTATTATTCC CGATTCCGCG 
AGTTTATTGA GCCCTTCAGC CACTGGCGCA GCAGAATGTG GCGCATCGAC GATGGAGTCC 
GCAGCGGCCT CCTCCGCCTC CGCCTCTTCC CCAGACCGGC CCGCCCCGGT AACAGCAGCG 
GCCGCGGGGG GACCGTCAGT CCTCGGCAGC TCCAGCTGTA GCAGCATTAG CTCCAGCGCG 
ACCGCGGGCA GCCAGAGCCC CGGATCCGGA GCCGCCAGTC CCGGGGAGGC GGTGTGCGGG 
GCTGGCGGGG CGTTCAGGGA GCTGTTCGAG GCCTGTCGGA ATGGAGATGT GTCGCGGGTG 
AAGAGGCTGG TGGACTCGGT GAATGTGAAC GCTAAAGACA TGGCCGGACG AAAATCAACC 
CCTCTGCATT TCGCTGCAGG CTTCGGCAGA AAGGATGTTG TCGAGCATCT CCTGCAAACC 
GGAGCCAACG TTCATTCCCG TGATGATGGT GGTCTGATCC CGCTCCATAA CGCTTGCTCA 
TTCGGCCACG CAGAAGTGGT CAGCCTGCTG TTGTGTAGCG GCGCCGACCC CAACGCTCGA 
GACAACTGGA ACTACACACC TCTGCATGAG GCCGCCATCA AAGGCAAAAT AGACGTCTGC 
ATCGTGTTGC TTCAGCACGG TGCTGATCCA AATATCAGAA ACACTGACGG CAAATCAGCT 
CTGGATCTGG CAGACCCTTC TGCTAAGACC GTCCTCACTG GTGAATATAA GAAGGATGAA 
CTATTGGAAG CAGCGAGGAG CGGAAATGAG GAAAAACTGA TGGCACTTTT GACCCCGCTT 
AACGTCAACT GTCATGCCAG TGATGGACGC AAGTCAACAT CACAAAAAAT GCTGTCCACT 
CCTCTCCACC TGGCCGCTGG GTATAATCGT GTGCGTATCG TGCAGTTATT GCTGCAGTAC 
GGTGCAGATG TCCATGCCAA AGACAAAGGT GGTTTGGTTC CTCTGCATAA TGCCTGTTCT 
TATGGTCACT ATGAGGTCAC AGAACTCCTG CTGAAGCATG GGGCGTGTGT TAATGCCATG 
GACCTCTGGC AGTTCACGCC GCTGCACGAG GCGGCCAGTA AGAATCGAGT GGAGGTTTGT 
TCTCTGCTGC TGAGTCACGG CGCAGATCCA ACACTGCTGA ACTGCCACGG CAAGAGTGCG 
GTGGATGTGG CTCCCACACC TGAGCTGAAA GAGAGACTCA CCTATGAATT TAAAGGACAC 
TCGCTTCTGC AAGCGGCCCG TGAGGCGGAC ATGGCGAAGG TCAAGAAAAC AGCTCAAGAA 
ATCATCAGCT TCAAACACCC GCACTCGCAC GACTCCGCTC TGCACTGCGC CGTAGCCTCT 
CCTCATCCCA AACGCAAACA GGTCACAGAG TTACTGCTGA GGAAAGGTGC CAACATTCAC 
GAGAAGAACA AGGATTTCAT GACCCCGTTT CACGTAGCAG CTGAACGAGG TCATAATGAT 
GTGCTTGAAG TCCTGCAGAA ACACGGAGCA AAGGTGAACG CTGCGGACAC ACTGGGTCAG 
ACGGCTCTGC ACAGGGCTGC TCTTGCTGGT CACATCCAGA CCTGCAGATT ACTGCTGAGT 
TACGGAGCAG ATCCTTCAAT CGTCTCACTG CAGGGCTTCA CTGCATCACA GATGGGGAAT 
GAAGCCGTTC AGCAAATACT CAATGAGAAC GTTCCTCCCC GTAATTCAGA TGTGGATTAC 
CGCTTGCTCG AAGCTGCTAA AGCTGGCGAC CTTGATACAG TGAAGCAACT GTGCTCTCCT 
CAGAACGTGA ACTGTCGGGA TCTGGAGGGC CGTCACTCTA CACCGCTACA CTTCGCCGCA 
GGCTACAACC GGGTGGCTGT GGTGGAGTAT CTGCTCCATC ACGGGGCCGA TGTGCACGCT 
AAAGATAAAG GGGGTCTGGT TCCTCTGCAC AACGCATGCT CGTATGGTCA CTACGAGGTG 
GCGGAGCTGC TGGTGAGACA TGGAGCATCT GTGAACGTGG CCGATCTCTG GAAGTTCACA 
CCACTACATG AGGCTGCAGC AAAGGGCAAA TACGAGATCT GCAAACTACT GCTCAAGCAT 
GGTGCAGACC CGTCTAAGAA GAACCGTGAT GGTAACATGG CTCTGGACAT GGTGAAAGAT 
GGAGACACGG ACATTCAAGA CCTGCTGCGT GGAGACGCCG CTCTGCTGGA CGCTGCCAAG 
AAGGGCTGTT TGGCCCGAGT GCAGAAACTC TGCAGCCCGG AGAACATCAA CTGCAGAGAC 
ACACAGGGCA GGAACTCCAC ACCGCTGCAC CTTGCGGCTG GCTATAATAA CTTGGAGGTG 
GCCGAGTATC TCCTGGAGCA TGGAGCGGAT GTGAACGCTC AGGATAAAGG AGGACTGATT 
CCTCTGCATA ATGCTGCTTC ATATGGGCAT GTGGACATCG CCGCCCTGCT GATCAAGTTC 
AACACGTGTG TAAATGCGAC AGATAAGTGG GCCTTCACTC CTTTGCATGA AGCTGCTCAG 
AAGGGCCGAA CACAGCTGTG TGCACTGCTA CTGGCACATG GTGCAAACCC CACCATGAAG 
AATCAAGAGG GACAGACTGC TCTGGACCTC GCCACGGCTG ATGACATCCG TGCTCTGCTG 
ATGGATGCGA TGCCCCCTGA TGCCCTGCCT AGCTGTTTCA AACCGCAGGC CACCGTGCTC 
AGCGCCGCGC TCATTTCTCC CGCCTCCACA CCCTCCTGCC TCTCCGCCGC CTCCAGCATC 
GACAACCTGG CTGGACCTCT ATGTGACGGA GCCAGTGGAG GAGCCGCGGG ACCCGCTGAT 
GGAGCCTCCA GCACTGATCG CAAAGAAGGA GATGGTGCGT TAATACAAGG AAACAAACTA 
ACTGCTGTCC TGGATATGAA CATCTCCCAG TTCCTGAAGA GTTTAGGCCT TGAACATTTG 
AGGGACATCT TTGAGAGAGA ACAGATCACC CTGGACGTAT TGGCTGATAT GGGTCATGAG 
GAACTCAAGG AGATAGGAAT CAACGCTTAC GGACACCGTC ATAAACTCAT TAAAGGCATT 
GAGAGGCTGC TGGGAGGACA GCAGGGTGCC AATCCATACC TGACGTTCCA CTGCACCAAT 
CAGGGCACAG TATTGATTGA CCTGGCTGTG GATGATAAGG AGTTTCAGTC CGTTGAGGAA 
GAGATGCAAA GCACAATTCG GGAACACAGG GATGGCGGAA ATGCAGGTGG CGTTTTCAGT 
CGTTACAATG TCATTAAGAT CCAGAAGGTG GTGAATAAGA AACTGAGAGA ACGATACACA 
CACAGGCAGA AAGAGATCGC GGACGAGAAT CACAATCACC ACAATGAGCG AATGCTGTTT 
CACGGATCTC CGTTTATAAA CGCCATCATC CATAAAGGCT TTGATGAGCG TCATGCGTAT 
ATCGGCGGCA TGTTTGGAGC GGGTATATAT TTTGCAGAGA ACTCTTCAAA GAGTAACCAG 
TACGTCTACG GCATCGGTGG TGGTACAGGA TGCCCAACTC ACAAGGATCG TTCCTGTTAC 
CTGTGCCACA GGCAAATGTT GTTCTGCCGT GTGACATTGG GAAAATCCTT CTTGCAGTTC 
AGTGCCATGA AGATGGCCCA CGCTCCGCCT GGGCACCACT CTGTGATCGG CCGTCCCAGT 
GTCAACGGCC TGGCCTACGC AGAGTATGTG ATCTATCGCG GAGAACAGGC CTACCCAGAG 
TATCTCATCA CCTATCAGAT AATGAAGCCG GACAGCACGC CTCAGTCCTC CACAGGAGCG 
GAGCAGAAAT CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171800.1
CDS	60..3914
Translation	MAVSRRSSQHQQSSLQPSPRNAVIASPPPESPSAIIPDSASLLSPSATGAAECGASTMESAAASSASASSPDRPAPVTAAAAGGPSVLGSSSCSSISSSATAGSQSPGSGAASPGEAVCGAGGAFRELFEACRNGDVSRVKRLVDSVNVNAKDMAGRKSTPLHFAAGFGRKDVVEHLLQTGANVHSRDDGGLIPLHNACSFGHAEVVSLLLCSGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLADPSAKTVLTGEYKKDELLEAARSGNEEKLMALLTPLNVNCHASDGRKSTSQKMLSTPLHLAAGYNRVRIVQLLLQYGADVHAKDKGGLVPLHNACSYGHYEVTELLLKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSHGADPTLLNCHGKSAVDVAPTPELKERLTYEFKGHSLLQAAREADMAKVKKTAQEIISFKHPHSHDSALHCAVASPHPKRKQVTELLLRKGANIHEKNKDFMTPFHVAAERGHNDVLEVLQKHGAKVNAADTLGQTALHRAALAGHIQTCRLLLSYGADPSIVSLQGFTASQMGNEAVQQILNENVPPRNSDVDYRLLEAAKAGDLDTVKQLCSPQNVNCRDLEGRHSTPLHFAAGYNRVAVVEYLLHHGADVHAKDKGGLVPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKGKYEICKLLLKHGADPSKKNRDGNMALDMVKDGDTDIQDLLRGDAALLDAAKKGCLARVQKLCSPENINCRDTQGRNSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYGHVDIAALLIKFNTCVNATDKWAFTPLHEAAQKGRTQLCALLLAHGANPTMKNQEGQTALDLATADDIRALLMDAMPPDALPSCFKPQATVLSAALISPASTPSCLSAASSIDNLAGPLCDGASGGAAGPADGASSTDRKEGDGALIQGNKLTAVLDMNISQFLKSLGLEHLRDIFEREQITLDVLADMGHEELKEIGINAYGHRHKLIKGIERLLGGQQGANPYLTFHCTNQGTVLIDLAVDDKEFQSVEEEMQSTIREHRDGGNAGGVFSRYNVIKIQKVVNKKLRERYTHRQKEIADENHNHHNERMLFHGSPFINAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPTHKDRSCYLCHRQMLFCRVTLGKSFLQFSAMKMAHAPPGHHSVIGRPSVNGLAYAEYVIYRGEQAYPEYLITYQIMKPDSTPQSSTGAEQKS

Target ORF information:

RefSeq Version	XM_005171743.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase b (tnksb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171743.4

ORF Insert Sequence:

ATGGCGGTGT CCCGTCGATC CTCACAGCAC CAACAAAGCA GCCTACAACC GTCTCCGCGA 
AACGCCGTAA TCGCTTCCCC TCCTCCTGAA TCGCCGTCCG CTATTATTCC CGATTCCGCG 
AGTTTATTGA GCCCTTCAGC CACTGGCGCA GCAGAATGTG GCGCATCGAC GATGGAGTCC 
GCAGCGGCCT CCTCCGCCTC CGCCTCTTCC CCAGACCGGC CCGCCCCGGT AACAGCAGCG 
GCCGCGGGGG GACCGTCAGT CCTCGGCAGC TCCAGCTGTA GCAGCATTAG CTCCAGCGCG 
ACCGCGGGCA GCCAGAGCCC CGGATCCGGA GCCGCCAGTC CCGGGGAGGC GGTGTGCGGG 
GCTGGCGGGG CGTTCAGGGA GCTGTTCGAG GCCTGTCGGA ATGGAGATGT GTCGCGGGTG 
AAGAGGCTGG TGGACTCGGT GAATGTGAAC GCTAAAGACA TGGCCGGACG AAAATCAACC 
CCTCTGCATT TCGCTGCAGG CTTCGGCAGA AAGGATGTTG TCGAGCATCT CCTGCAAACC 
GGAGCCAACG TTCATTCCCG TGATGATGGT GGTCTGATCC CGCTCCATAA CGCTTGCTCA 
TTCGGCCACG CAGAAGTGGT CAGCCTGCTG TTGTGTAGCG GCGCCGACCC CAACGCTCGA 
GACAACTGGA ACTACACACC TCTGCATGAG GCCGCCATCA AAGGCAAAAT AGACGTCTGC 
ATCGTGTTGC TTCAGCACGG TGCTGATCCA AATATCAGAA ACACTGACGG CAAATCAGCT 
CTGGATCTGG CAGACCCTTC TGCTAAGACC GTCCTCACTG GTGAATATAA GAAGGATGAA 
CTATTGGAAG CAGCGAGGAG CGGAAATGAG GAAAAACTGA TGGCACTTTT GACCCCGCTT 
AACGTCAACT GTCATGCCAG TGATGGACGC AAGTCAACAT CACAAAAAAT GCTGTCCACT 
CCTCTCCACC TGGCCGCTGG GTATAATCGT GTGCGTATCG TGCAGTTATT GCTGCAGTAC 
GGTGCAGATG TCCATGCCAA AGACAAAGGT GGTTTGGTTC CTCTGCATAA TGCCTGTTCT 
TATGGTCACT ATGAGGTCAC AGAACTCCTG CTGAAGCATG GGGCGTGTGT TAATGCCATG 
GACCTCTGGC AGTTCACGCC GCTGCACGAG GCGGCCAGTA AGAATCGAGT GGAGGTTTGT 
TCTCTGCTGC TGAGTCACGG CGCAGATCCA ACACTGCTGA ACTGCCACGG CAAGAGTGCG 
GTGGATGTGG CTCCCACACC TGAGCTGAAA GAGAGACTCA CCTATGAATT TAAAGGACAC 
TCGCTTCTGC AAGCGGCCCG TGAGGCGGAC ATGGCGAAGG TCAAGAAAAC AGCTCAAGAA 
ATCATCAGCT TCAAACACCC GCACTCGCAC GACTCCGCTC TGCACTGCGC CGTAGCCTCT 
CCTCATCCCA AACGCAAACA GGTCACAGAG TTACTGCTGA GGAAAGGTGC CAACATTCAC 
GAGAAGAACA AGGATTTCAT GACCCCGTTT CACGTAGCAG CTGAACGAGG TCATAATGAT 
GTGCTTGAAG TCCTGCAGAA ACACGGAGCA AAGGTGAACG CTGCGGACAC ACTGGGTCAG 
ACGGCTCTGC ACAGGGCTGC TCTTGCTGGT CACATCCAGA CCTGCAGATT ACTGCTGAGT 
TACGGAGCAG ATCCTTCAAT CGTCTCACTG CAGGGCTTCA CTGCATCACA GATGGGGAAT 
GAAGCCGTTC AGCAAATACT CAATGAGAAC GTTCCTCCCC GTAATTCAGA TGTGGATTAC 
CGCTTGCTCG AAGCTGCTAA AGCTGGCGAC CTTGATACAG TGAAGCAACT GTGCTCTCCT 
CAGAACGTGA ACTGTCGGGA TCTGGAGGGC CGTCACTCTA CACCGCTACA CTTCGCCGCA 
GGCTACAACC GGGTGGCTGT GGTGGAGTAT CTGCTCCATC ACGGGGCCGA TGTGCACGCT 
AAAGATAAAG GGGGTCTGGT TCCTCTGCAC AACGCATGCT CGTATGGTCA CTACGAGGTG 
GCGGAGCTGC TGGTGAGACA TGGAGCATCT GTGAACGTGG CCGATCTCTG GAAGTTCACA 
CCACTACATG AGGCTGCAGC AAAGGGCAAA TACGAGATCT GCAAACTACT GCTCAAGCAT 
GGTGCAGACC CGTCTAAGAA GAACCGTGAT GGTAACATGG CTCTGGACAT GGTGAAAGAT 
GGAGACACGG ACATTCAAGA CCTGCTGCGT GGAGACGCCG CTCTGCTGGA CGCTGCCAAG 
AAGGGCTGTT TGGCCCGAGT GCAGAAACTC TGCAGCCCGG AGAACATCAA CTGCAGAGAC 
ACACAGGGCA GGAACTCCAC ACCGCTGCAC CTTGCGGCTG GCTATAATAA CTTGGAGGTG 
GCCGAGTATC TCCTGGAGCA TGGAGCGGAT GTGAACGCTC AGGATAAAGG AGGACTGATT 
CCTCTGCATA ATGCTGCTTC ATATGGGCAT GTGGACATCG CCGCCCTGCT GATCAAGTTC 
AACACGTGTG TAAATGCGAC AGATAAGTGG GCCTTCACTC CTTTGCATGA AGCTGCTCAG 
AAGGGCCGAA CACAGCTGTG TGCACTGCTA CTGGCACATG GTGCAAACCC CACCATGAAG 
AATCAAGAGG GACAGACTGC TCTGGACCTC GCCACGGCTG ATGACATCCG TGCTCTGCTG 
ATGGATGCGA TGCCCCCTGA TGCCCTGCCT AGCTGTTTCA AACCGCAGGC CACCGTGCTC 
AGCGCCGCGC TCATTTCTCC CGCCTCCACA CCCTCCTGCC TCTCCGCCGC CTCCAGCATC 
GACAACCTGG CTGGACCTCT ATGTGACGGA GCCAGTGGAG GAGCCGCGGG ACCCGCTGAT 
GGAGCCTCCA GCACTGATCG CAAAGAAGGA GATGGTGCGT TAATACAAGG AAACAAACTA 
ACTGCTGTCC TGGATATGAA CATCTCCCAG TTCCTGAAGA GTTTAGGCCT TGAACATTTG 
AGGGACATCT TTGAGAGAGA ACAGATCACC CTGGACGTAT TGGCTGATAT GGGTCATGAG 
GAACTCAAGG AGATAGGAAT CAACGCTTAC GGACACCGTC ATAAACTCAT TAAAGGCATT 
GAGAGGCTGC TGGGAGGACA GCAGGGTGCC AATCCATACC TGACGTTCCA CTGCACCAAT 
CAGGGCACAG TATTGATTGA CCTGGCTGTG GATGATAAGG AGTTTCAGTC CGTTGAGGAA 
GAGATGCAAA GCACAATTCG GGAACACAGG GATGGCGGAA ATGCAGGTGG CGTTTTCAGT 
CGTTACAATG TCATTAAGAT CCAGAAGGTG GTGAATAAGA AACTGAGAGA ACGATACACA 
CACAGGCAGA AAGAGATCGC GGACGAGAAT CACAATCACC ACAATGAGCG AATGCTGTTT 
CACGGATCTC CGTTTATAAA CGCCATCATC CATAAAGGCT TTGATGAGCG TCATGCGTAT 
ATCGGCGGCA TGTTTGGAGC GGGTATATAT TTTGCAGAGA ACTCTTCAAA GAGTAACCAG 
TACGTCTACG GCATCGGTGG TGGTACAGGA TGCCCAACTC ACAAGGATCG TTCCTGTTAC 
CTGTGCCACA GGCAAATGTT GTTCTGCCGT GTGACATTGG GAAAATCCTT CTTGCAGTTC 
AGTGCCATGA AGATGGCCCA CGCTCCGCCT GGGCACCACT CTGTGATCGG CCGTCCCAGT 
GTCAACGGCC TGGCCTACGC AGAGTATGTG ATCTATCGCG GAGAACAGGC CTACCCAGAG 
TATCTCATCA CCTATCAGAT AATGAAGCCG GACAGCACGC CTCAGTCCTC CACAGGAGCG 
GAGCAGAAAT CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnksb ( XM_005171744.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171801.1 Danio rerio tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase b (tnksb), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa10084
Clone ID Related Accession (Same CDS sequence)	XM_005171744.3 , XM_005171744.4
Accession Version	XM_005171744.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tankyrase 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005171744.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGT CCCGTCGATC CTCACAGCAC CAACAAAGCA GCCTACAACC GTCTCCGCGA 
AACGCCGTAA TCGCTTCCCC TCCTCCTGAA TCGCCGTCCG CTATTATTCC CGATTCCGCG 
AGTTTATTGA GCCCTTCAGC CACTGGCGCA GCAGAATGTG GCGCATCGAC GATGGAGTCC 
GCAGCGGCCT CCTCCGCCTC CGCCTCTTCC CCAGACCGGC CCGCCCCGGT AACAGCAGCG 
GCCGCGGGGG GACCGTCAGT CCTCGGCAGC TCCAGCTGTA GCAGCATTAG CTCCAGCGCG 
ACCGCGGGCA GCCAGAGCCC CGGATCCGGA GCCGCCAGTC CCGGGGAGGC GGTGTGCGGG 
GCTGGCGGGG CGTTCAGGGA GCTGTTCGAG GCCTGTCGGA ATGGAGATGT GTCGCGGGTG 
AAGAGGCTGG TGGACTCGGT GAATGTGAAC GCTAAAGACA TGGCCGGACG AAAATCAACC 
CCTCTGCATT TCGCTGCAGG CTTCGGCAGA AAGGATGTTG TCGAGCATCT CCTGCAAACC 
GGAGCCAACG TTCATTCCCG TGATGATGGT GGTCTGATCC CGCTCCATAA CGCTTGCTCA 
TTCGGCCACG CAGAAGTGGT CAGCCTGCTG TTGTGTAGCG GCGCCGACCC CAACGCTCGA 
GACAACTGGA ACTACACACC TCTGCATGAG GCCGCCATCA AAGGCAAAAT AGACGTCTGC 
ATCGTGTTGC TTCAGCACGG TGCTGATCCA AATATCAGAA ACACTGACGG CAAATCAGCT 
CTGGATCTGG CAGACCCTTC TGCTAAGACC GTCCTCACTG GTGAATATAA GAAGGATGAA 
CTATTGGAAG CAGCGAGGAG CGGAAATGAG GAAAAACTGA TGGCACTTTT GACCCCGCTT 
AACGTCAACT GTCATGCCAG TGATGGACGC AAGTCCACTC CTCTCCACCT GGCCGCTGGG 
TATAATCGTG TGCGTATCGT GCAGTTATTG CTGCAGTACG GTGCAGATGT CCATGCCAAA 
GACAAAGGTG GTTTGGTTCC TCTGCATAAT GCCTGTTCTT ATGGTCACTA TGAGGTCACA 
GAACTCCTGC TGAAGCATGG GGCGTGTGTT AATGCCATGG ACCTCTGGCA GTTCACGCCG 
CTGCACGAGG CGGCCAGTAA GAATCGAGTG GAGGTTTGTT CTCTGCTGCT GAGTCACGGC 
GCAGATCCAA CACTGCTGAA CTGCCACGGC AAGAGTGCGG TGGATGTGGC TCCCACACCT 
GAGCTGAAAG AGAGACTCAC CTATGAATTT AAAGGACACT CGCTTCTGCA AGCGGCCCGT 
GAGGCGGACA TGGCGAAGGT CAAGAAAACA GCTCAAGAAA TCATCAGCTT CAAACACCCG 
CACTCGCACG ACTCCGCTCT GCACTGCGCC GTAGCCTCTC CTCATCCCAA ACGCAAACAG 
GTCACAGAGT TACTGCTGAG GAAAGGTGCC AACATTCACG AGAAGAACAA GGATTTCATG 
ACCCCGTTTC ACGTAGCAGC TGAACGAGGT CATAATGATG TGCTTGAAGT CCTGCAGAAA 
CACGGAGCAA AGGTGAACGC TGCGGACACA CTGGGTCAGA CGGCTCTGCA CAGGGCTGCT 
CTTGCTGGTC ACATCCAGAC CTGCAGATTA CTGCTGAGTT ACGGAGCAGA TCCTTCAATC 
GTCTCACTGC AGGGCTTCAC TGCATCACAG ATGGGGAATG AAGCCGTTCA GCAAATACTC 
AATGAGAACG TTCCTCCCCG TAATTCAGAT GTGGATTACC GCTTGCTCGA AGCTGCTAAA 
GCTGGCGACC TTGATACAGT GAAGCAACTG TGCTCTCCTC AGAACGTGAA CTGTCGGGAT 
CTGGAGGGCC GTCACTCTAC ACCGCTACAC TTCGCCGCAG GCTACAACCG GGTGGCTGTG 
GTGGAGTATC TGCTCCATCA CGGGGCCGAT GTGCACGCTA AAGATAAAGG GGGTCTGGTT 
CCTCTGCACA ACGCATGCTC GTATGGTCAC TACGAGGTGG CGGAGCTGCT GGTGAGACAT 
GGAGCATCTG TGAACGTGGC CGATCTCTGG AAGTTCACAC CACTACATGA GGCTGCAGCA 
AAGGGCAAAT ACGAGATCTG CAAACTACTG CTCAAGCATG GTGCAGACCC GTCTAAGAAG 
AACCGTGATG GTAACATGGC TCTGGACATG GTGAAAGATG GAGACACGGA CATTCAAGAC 
CTGCTGCGTG GAGACGCCGC TCTGCTGGAC GCTGCCAAGA AGGGCTGTTT GGCCCGAGTG 
CAGAAACTCT GCAGCCCGGA GAACATCAAC TGCAGAGACA CACAGGGCAG GAACTCCACA 
CCGCTGCACC TTGCGGCTGG CTATAATAAC TTGGAGGTGG CCGAGTATCT CCTGGAGCAT 
GGAGCGGATG TGAACGCTCA GGATAAAGGA GGACTGATTC CTCTGCATAA TGCTGCTTCA 
TATGGGCATG TGGACATCGC CGCCCTGCTG ATCAAGTTCA ACACGTGTGT AAATGCGACA 
GATAAGTGGG CCTTCACTCC TTTGCATGAA GCTGCTCAGA AGGGCCGAAC ACAGCTGTGT 
GCACTGCTAC TGGCACATGG TGCAAACCCC ACCATGAAGA ATCAAGAGGG ACAGACTGCT 
CTGGACCTCG CCACGGCTGA TGACATCCGT GCTCTGCTGA TGGATGCGAT GCCCCCTGAT 
GCCCTGCCTA GCTGTTTCAA ACCGCAGGCC ACCGTGCTCA GCGCCGCGCT CATTTCTCCC 
GCCTCCACAC CCTCCTGCCT CTCCGCCGCC TCCAGCATCG ACAACCTGGC TGGACCTCTA 
TGTGACGGAG CCAGTGGAGG AGCCGCGGGA CCCGCTGATG GAGCCTCCAG CACTGATCGC 
AAAGAAGGAG ATGGTGCGTT AATACAAGGA AACAAACTAA CTGCTGTCCT GGATATGAAC 
ATCTCCCAGT TCCTGAAGAG TTTAGGCCTT GAACATTTGA GGGACATCTT TGAGAGAGAA 
CAGATCACCC TGGACGTATT GGCTGATATG GGTCATGAGG AACTCAAGGA GATAGGAATC 
AACGCTTACG GACACCGTCA TAAACTCATT AAAGGCATTG AGAGGCTGCT GGGAGGACAG 
CAGGGTGCCA ATCCATACCT GACGTTCCAC TGCACCAATC AGGGCACAGT ATTGATTGAC 
CTGGCTGTGG ATGATAAGGA GTTTCAGTCC GTTGAGGAAG AGATGCAAAG CACAATTCGG 
GAACACAGGG ATGGCGGAAA TGCAGGTGGC GTTTTCAGTC GTTACAATGT CATTAAGATC 
CAGAAGGTGG TGAATAAGAA ACTGAGAGAA CGATACACAC ACAGGCAGAA AGAGATCGCG 
GACGAGAATC ACAATCACCA CAATGAGCGA ATGCTGTTTC ACGGATCTCC GTTTATAAAC 
GCCATCATCC ATAAAGGCTT TGATGAGCGT CATGCGTATA TCGGCGGCAT GTTTGGAGCG 
GGTATATATT TTGCAGAGAA CTCTTCAAAG AGTAACCAGT ACGTCTACGG CATCGGTGGT 
GGTACAGGAT GCCCAACTCA CAAGGATCGT TCCTGTTACC TGTGCCACAG GCAAATGTTG 
TTCTGCCGTG TGACATTGGG AAAATCCTTC TTGCAGTTCA GTGCCATGAA GATGGCCCAC 
GCTCCGCCTG GGCACCACTC TGTGATCGGC CGTCCCAGTG TCAACGGCCT GGCCTACGCA 
GAGTATGTGA TCTATCGCGG AGAACAGGCC TACCCAGAGT ATCTCATCAC CTATCAGATA 
ATGAAGCCGG ACAGCACGCC TCAGTCCTCC ACAGGAGCGG AGCAGAAATC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171801.1
CDS	76..3909
Translation	MAVSRRSSQHQQSSLQPSPRNAVIASPPPESPSAIIPDSASLLSPSATGAAECGASTMESAAASSASASSPDRPAPVTAAAAGGPSVLGSSSCSSISSSATAGSQSPGSGAASPGEAVCGAGGAFRELFEACRNGDVSRVKRLVDSVNVNAKDMAGRKSTPLHFAAGFGRKDVVEHLLQTGANVHSRDDGGLIPLHNACSFGHAEVVSLLLCSGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLADPSAKTVLTGEYKKDELLEAARSGNEEKLMALLTPLNVNCHASDGRKSTPLHLAAGYNRVRIVQLLLQYGADVHAKDKGGLVPLHNACSYGHYEVTELLLKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSHGADPTLLNCHGKSAVDVAPTPELKERLTYEFKGHSLLQAAREADMAKVKKTAQEIISFKHPHSHDSALHCAVASPHPKRKQVTELLLRKGANIHEKNKDFMTPFHVAAERGHNDVLEVLQKHGAKVNAADTLGQTALHRAALAGHIQTCRLLLSYGADPSIVSLQGFTASQMGNEAVQQILNENVPPRNSDVDYRLLEAAKAGDLDTVKQLCSPQNVNCRDLEGRHSTPLHFAAGYNRVAVVEYLLHHGADVHAKDKGGLVPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKGKYEICKLLLKHGADPSKKNRDGNMALDMVKDGDTDIQDLLRGDAALLDAAKKGCLARVQKLCSPENINCRDTQGRNSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYGHVDIAALLIKFNTCVNATDKWAFTPLHEAAQKGRTQLCALLLAHGANPTMKNQEGQTALDLATADDIRALLMDAMPPDALPSCFKPQATVLSAALISPASTPSCLSAASSIDNLAGPLCDGASGGAAGPADGASSTDRKEGDGALIQGNKLTAVLDMNISQFLKSLGLEHLRDIFEREQITLDVLADMGHEELKEIGINAYGHRHKLIKGIERLLGGQQGANPYLTFHCTNQGTVLIDLAVDDKEFQSVEEEMQSTIREHRDGGNAGGVFSRYNVIKIQKVVNKKLRERYTHRQKEIADENHNHHNERMLFHGSPFINAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPTHKDRSCYLCHRQMLFCRVTLGKSFLQFSAMKMAHAPPGHHSVIGRPSVNGLAYAEYVIYRGEQAYPEYLITYQIMKPDSTPQSSTGAEQKS

Target ORF information:

RefSeq Version	XM_005171744.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase b (tnksb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171744.3

ORF Insert Sequence:

ATGGCGGTGT CCCGTCGATC CTCACAGCAC CAACAAAGCA GCCTACAACC GTCTCCGCGA 
AACGCCGTAA TCGCTTCCCC TCCTCCTGAA TCGCCGTCCG CTATTATTCC CGATTCCGCG 
AGTTTATTGA GCCCTTCAGC CACTGGCGCA GCAGAATGTG GCGCATCGAC GATGGAGTCC 
GCAGCGGCCT CCTCCGCCTC CGCCTCTTCC CCAGACCGGC CCGCCCCGGT AACAGCAGCG 
GCCGCGGGGG GACCGTCAGT CCTCGGCAGC TCCAGCTGTA GCAGCATTAG CTCCAGCGCG 
ACCGCGGGCA GCCAGAGCCC CGGATCCGGA GCCGCCAGTC CCGGGGAGGC GGTGTGCGGG 
GCTGGCGGGG CGTTCAGGGA GCTGTTCGAG GCCTGTCGGA ATGGAGATGT GTCGCGGGTG 
AAGAGGCTGG TGGACTCGGT GAATGTGAAC GCTAAAGACA TGGCCGGACG AAAATCAACC 
CCTCTGCATT TCGCTGCAGG CTTCGGCAGA AAGGATGTTG TCGAGCATCT CCTGCAAACC 
GGAGCCAACG TTCATTCCCG TGATGATGGT GGTCTGATCC CGCTCCATAA CGCTTGCTCA 
TTCGGCCACG CAGAAGTGGT CAGCCTGCTG TTGTGTAGCG GCGCCGACCC CAACGCTCGA 
GACAACTGGA ACTACACACC TCTGCATGAG GCCGCCATCA AAGGCAAAAT AGACGTCTGC 
ATCGTGTTGC TTCAGCACGG TGCTGATCCA AATATCAGAA ACACTGACGG CAAATCAGCT 
CTGGATCTGG CAGACCCTTC TGCTAAGACC GTCCTCACTG GTGAATATAA GAAGGATGAA 
CTATTGGAAG CAGCGAGGAG CGGAAATGAG GAAAAACTGA TGGCACTTTT GACCCCGCTT 
AACGTCAACT GTCATGCCAG TGATGGACGC AAGTCCACTC CTCTCCACCT GGCCGCTGGG 
TATAATCGTG TGCGTATCGT GCAGTTATTG CTGCAGTACG GTGCAGATGT CCATGCCAAA 
GACAAAGGTG GTTTGGTTCC TCTGCATAAT GCCTGTTCTT ATGGTCACTA TGAGGTCACA 
GAACTCCTGC TGAAGCATGG GGCGTGTGTT AATGCCATGG ACCTCTGGCA GTTCACGCCG 
CTGCACGAGG CGGCCAGTAA GAATCGAGTG GAGGTTTGTT CTCTGCTGCT GAGTCACGGC 
GCAGATCCAA CACTGCTGAA CTGCCACGGC AAGAGTGCGG TGGATGTGGC TCCCACACCT 
GAGCTGAAAG AGAGACTCAC CTATGAATTT AAAGGACACT CGCTTCTGCA AGCGGCCCGT 
GAGGCGGACA TGGCGAAGGT CAAGAAAACA GCTCAAGAAA TCATCAGCTT CAAACACCCG 
CACTCGCACG ACTCCGCTCT GCACTGCGCC GTAGCCTCTC CTCATCCCAA ACGCAAACAG 
GTCACAGAGT TACTGCTGAG GAAAGGTGCC AACATTCACG AGAAGAACAA GGATTTCATG 
ACCCCGTTTC ACGTAGCAGC TGAACGAGGT CATAATGATG TGCTTGAAGT CCTGCAGAAA 
CACGGAGCAA AGGTGAACGC TGCGGACACA CTGGGTCAGA CGGCTCTGCA CAGGGCTGCT 
CTTGCTGGTC ACATCCAGAC CTGCAGATTA CTGCTGAGTT ACGGAGCAGA TCCTTCAATC 
GTCTCACTGC AGGGCTTCAC TGCATCACAG ATGGGGAATG AAGCCGTTCA GCAAATACTC 
AATGAGAACG TTCCTCCCCG TAATTCAGAT GTGGATTACC GCTTGCTCGA AGCTGCTAAA 
GCTGGCGACC TTGATACAGT GAAGCAACTG TGCTCTCCTC AGAACGTGAA CTGTCGGGAT 
CTGGAGGGCC GTCACTCTAC ACCGCTACAC TTCGCCGCAG GCTACAACCG GGTGGCTGTG 
GTGGAGTATC TGCTCCATCA CGGGGCCGAT GTGCACGCTA AAGATAAAGG GGGTCTGGTT 
CCTCTGCACA ACGCATGCTC GTATGGTCAC TACGAGGTGG CGGAGCTGCT GGTGAGACAT 
GGAGCATCTG TGAACGTGGC CGATCTCTGG AAGTTCACAC CACTACATGA GGCTGCAGCA 
AAGGGCAAAT ACGAGATCTG CAAACTACTG CTCAAGCATG GTGCAGACCC GTCTAAGAAG 
AACCGTGATG GTAACATGGC TCTGGACATG GTGAAAGATG GAGACACGGA CATTCAAGAC 
CTGCTGCGTG GAGACGCCGC TCTGCTGGAC GCTGCCAAGA AGGGCTGTTT GGCCCGAGTG 
CAGAAACTCT GCAGCCCGGA GAACATCAAC TGCAGAGACA CACAGGGCAG GAACTCCACA 
CCGCTGCACC TTGCGGCTGG CTATAATAAC TTGGAGGTGG CCGAGTATCT CCTGGAGCAT 
GGAGCGGATG TGAACGCTCA GGATAAAGGA GGACTGATTC CTCTGCATAA TGCTGCTTCA 
TATGGGCATG TGGACATCGC CGCCCTGCTG ATCAAGTTCA ACACGTGTGT AAATGCGACA 
GATAAGTGGG CCTTCACTCC TTTGCATGAA GCTGCTCAGA AGGGCCGAAC ACAGCTGTGT 
GCACTGCTAC TGGCACATGG TGCAAACCCC ACCATGAAGA ATCAAGAGGG ACAGACTGCT 
CTGGACCTCG CCACGGCTGA TGACATCCGT GCTCTGCTGA TGGATGCGAT GCCCCCTGAT 
GCCCTGCCTA GCTGTTTCAA ACCGCAGGCC ACCGTGCTCA GCGCCGCGCT CATTTCTCCC 
GCCTCCACAC CCTCCTGCCT CTCCGCCGCC TCCAGCATCG ACAACCTGGC TGGACCTCTA 
TGTGACGGAG CCAGTGGAGG AGCCGCGGGA CCCGCTGATG GAGCCTCCAG CACTGATCGC 
AAAGAAGGAG ATGGTGCGTT AATACAAGGA AACAAACTAA CTGCTGTCCT GGATATGAAC 
ATCTCCCAGT TCCTGAAGAG TTTAGGCCTT GAACATTTGA GGGACATCTT TGAGAGAGAA 
CAGATCACCC TGGACGTATT GGCTGATATG GGTCATGAGG AACTCAAGGA GATAGGAATC 
AACGCTTACG GACACCGTCA TAAACTCATT AAAGGCATTG AGAGGCTGCT GGGAGGACAG 
CAGGGTGCCA ATCCATACCT GACGTTCCAC TGCACCAATC AGGGCACAGT ATTGATTGAC 
CTGGCTGTGG ATGATAAGGA GTTTCAGTCC GTTGAGGAAG AGATGCAAAG CACAATTCGG 
GAACACAGGG ATGGCGGAAA TGCAGGTGGC GTTTTCAGTC GTTACAATGT CATTAAGATC 
CAGAAGGTGG TGAATAAGAA ACTGAGAGAA CGATACACAC ACAGGCAGAA AGAGATCGCG 
GACGAGAATC ACAATCACCA CAATGAGCGA ATGCTGTTTC ACGGATCTCC GTTTATAAAC 
GCCATCATCC ATAAAGGCTT TGATGAGCGT CATGCGTATA TCGGCGGCAT GTTTGGAGCG 
GGTATATATT TTGCAGAGAA CTCTTCAAAG AGTAACCAGT ACGTCTACGG CATCGGTGGT 
GGTACAGGAT GCCCAACTCA CAAGGATCGT TCCTGTTACC TGTGCCACAG GCAAATGTTG 
TTCTGCCGTG TGACATTGGG AAAATCCTTC TTGCAGTTCA GTGCCATGAA GATGGCCCAC 
GCTCCGCCTG GGCACCACTC TGTGATCGGC CGTCCCAGTG TCAACGGCCT GGCCTACGCA 
GAGTATGTGA TCTATCGCGG AGAACAGGCC TACCCAGAGT ATCTCATCAC CTATCAGATA 
ATGAAGCCGG ACAGCACGCC TCAGTCCTCC ACAGGAGCGG AGCAGAAATC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnksb ( XM_005171744.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171801.1 Danio rerio tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase b (tnksb), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa10084
Clone ID Related Accession (Same CDS sequence)	XM_005171744.3 , XM_005171744.4
Accession Version	XM_005171744.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tankyrase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005171744.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGT CCCGTCGATC CTCACAGCAC CAACAAAGCA GCCTACAACC GTCTCCGCGA 
AACGCCGTAA TCGCTTCCCC TCCTCCTGAA TCGCCGTCCG CTATTATTCC CGATTCCGCG 
AGTTTATTGA GCCCTTCAGC CACTGGCGCA GCAGAATGTG GCGCATCGAC GATGGAGTCC 
GCAGCGGCCT CCTCCGCCTC CGCCTCTTCC CCAGACCGGC CCGCCCCGGT AACAGCAGCG 
GCCGCGGGGG GACCGTCAGT CCTCGGCAGC TCCAGCTGTA GCAGCATTAG CTCCAGCGCG 
ACCGCGGGCA GCCAGAGCCC CGGATCCGGA GCCGCCAGTC CCGGGGAGGC GGTGTGCGGG 
GCTGGCGGGG CGTTCAGGGA GCTGTTCGAG GCCTGTCGGA ATGGAGATGT GTCGCGGGTG 
AAGAGGCTGG TGGACTCGGT GAATGTGAAC GCTAAAGACA TGGCCGGACG AAAATCAACC 
CCTCTGCATT TCGCTGCAGG CTTCGGCAGA AAGGATGTTG TCGAGCATCT CCTGCAAACC 
GGAGCCAACG TTCATTCCCG TGATGATGGT GGTCTGATCC CGCTCCATAA CGCTTGCTCA 
TTCGGCCACG CAGAAGTGGT CAGCCTGCTG TTGTGTAGCG GCGCCGACCC CAACGCTCGA 
GACAACTGGA ACTACACACC TCTGCATGAG GCCGCCATCA AAGGCAAAAT AGACGTCTGC 
ATCGTGTTGC TTCAGCACGG TGCTGATCCA AATATCAGAA ACACTGACGG CAAATCAGCT 
CTGGATCTGG CAGACCCTTC TGCTAAGACC GTCCTCACTG GTGAATATAA GAAGGATGAA 
CTATTGGAAG CAGCGAGGAG CGGAAATGAG GAAAAACTGA TGGCACTTTT GACCCCGCTT 
AACGTCAACT GTCATGCCAG TGATGGACGC AAGTCCACTC CTCTCCACCT GGCCGCTGGG 
TATAATCGTG TGCGTATCGT GCAGTTATTG CTGCAGTACG GTGCAGATGT CCATGCCAAA 
GACAAAGGTG GTTTGGTTCC TCTGCATAAT GCCTGTTCTT ATGGTCACTA TGAGGTCACA 
GAACTCCTGC TGAAGCATGG GGCGTGTGTT AATGCCATGG ACCTCTGGCA GTTCACGCCG 
CTGCACGAGG CGGCCAGTAA GAATCGAGTG GAGGTTTGTT CTCTGCTGCT GAGTCACGGC 
GCAGATCCAA CACTGCTGAA CTGCCACGGC AAGAGTGCGG TGGATGTGGC TCCCACACCT 
GAGCTGAAAG AGAGACTCAC CTATGAATTT AAAGGACACT CGCTTCTGCA AGCGGCCCGT 
GAGGCGGACA TGGCGAAGGT CAAGAAAACA GCTCAAGAAA TCATCAGCTT CAAACACCCG 
CACTCGCACG ACTCCGCTCT GCACTGCGCC GTAGCCTCTC CTCATCCCAA ACGCAAACAG 
GTCACAGAGT TACTGCTGAG GAAAGGTGCC AACATTCACG AGAAGAACAA GGATTTCATG 
ACCCCGTTTC ACGTAGCAGC TGAACGAGGT CATAATGATG TGCTTGAAGT CCTGCAGAAA 
CACGGAGCAA AGGTGAACGC TGCGGACACA CTGGGTCAGA CGGCTCTGCA CAGGGCTGCT 
CTTGCTGGTC ACATCCAGAC CTGCAGATTA CTGCTGAGTT ACGGAGCAGA TCCTTCAATC 
GTCTCACTGC AGGGCTTCAC TGCATCACAG ATGGGGAATG AAGCCGTTCA GCAAATACTC 
AATGAGAACG TTCCTCCCCG TAATTCAGAT GTGGATTACC GCTTGCTCGA AGCTGCTAAA 
GCTGGCGACC TTGATACAGT GAAGCAACTG TGCTCTCCTC AGAACGTGAA CTGTCGGGAT 
CTGGAGGGCC GTCACTCTAC ACCGCTACAC TTCGCCGCAG GCTACAACCG GGTGGCTGTG 
GTGGAGTATC TGCTCCATCA CGGGGCCGAT GTGCACGCTA AAGATAAAGG GGGTCTGGTT 
CCTCTGCACA ACGCATGCTC GTATGGTCAC TACGAGGTGG CGGAGCTGCT GGTGAGACAT 
GGAGCATCTG TGAACGTGGC CGATCTCTGG AAGTTCACAC CACTACATGA GGCTGCAGCA 
AAGGGCAAAT ACGAGATCTG CAAACTACTG CTCAAGCATG GTGCAGACCC GTCTAAGAAG 
AACCGTGATG GTAACATGGC TCTGGACATG GTGAAAGATG GAGACACGGA CATTCAAGAC 
CTGCTGCGTG GAGACGCCGC TCTGCTGGAC GCTGCCAAGA AGGGCTGTTT GGCCCGAGTG 
CAGAAACTCT GCAGCCCGGA GAACATCAAC TGCAGAGACA CACAGGGCAG GAACTCCACA 
CCGCTGCACC TTGCGGCTGG CTATAATAAC TTGGAGGTGG CCGAGTATCT CCTGGAGCAT 
GGAGCGGATG TGAACGCTCA GGATAAAGGA GGACTGATTC CTCTGCATAA TGCTGCTTCA 
TATGGGCATG TGGACATCGC CGCCCTGCTG ATCAAGTTCA ACACGTGTGT AAATGCGACA 
GATAAGTGGG CCTTCACTCC TTTGCATGAA GCTGCTCAGA AGGGCCGAAC ACAGCTGTGT 
GCACTGCTAC TGGCACATGG TGCAAACCCC ACCATGAAGA ATCAAGAGGG ACAGACTGCT 
CTGGACCTCG CCACGGCTGA TGACATCCGT GCTCTGCTGA TGGATGCGAT GCCCCCTGAT 
GCCCTGCCTA GCTGTTTCAA ACCGCAGGCC ACCGTGCTCA GCGCCGCGCT CATTTCTCCC 
GCCTCCACAC CCTCCTGCCT CTCCGCCGCC TCCAGCATCG ACAACCTGGC TGGACCTCTA 
TGTGACGGAG CCAGTGGAGG AGCCGCGGGA CCCGCTGATG GAGCCTCCAG CACTGATCGC 
AAAGAAGGAG ATGGTGCGTT AATACAAGGA AACAAACTAA CTGCTGTCCT GGATATGAAC 
ATCTCCCAGT TCCTGAAGAG TTTAGGCCTT GAACATTTGA GGGACATCTT TGAGAGAGAA 
CAGATCACCC TGGACGTATT GGCTGATATG GGTCATGAGG AACTCAAGGA GATAGGAATC 
AACGCTTACG GACACCGTCA TAAACTCATT AAAGGCATTG AGAGGCTGCT GGGAGGACAG 
CAGGGTGCCA ATCCATACCT GACGTTCCAC TGCACCAATC AGGGCACAGT ATTGATTGAC 
CTGGCTGTGG ATGATAAGGA GTTTCAGTCC GTTGAGGAAG AGATGCAAAG CACAATTCGG 
GAACACAGGG ATGGCGGAAA TGCAGGTGGC GTTTTCAGTC GTTACAATGT CATTAAGATC 
CAGAAGGTGG TGAATAAGAA ACTGAGAGAA CGATACACAC ACAGGCAGAA AGAGATCGCG 
GACGAGAATC ACAATCACCA CAATGAGCGA ATGCTGTTTC ACGGATCTCC GTTTATAAAC 
GCCATCATCC ATAAAGGCTT TGATGAGCGT CATGCGTATA TCGGCGGCAT GTTTGGAGCG 
GGTATATATT TTGCAGAGAA CTCTTCAAAG AGTAACCAGT ACGTCTACGG CATCGGTGGT 
GGTACAGGAT GCCCAACTCA CAAGGATCGT TCCTGTTACC TGTGCCACAG GCAAATGTTG 
TTCTGCCGTG TGACATTGGG AAAATCCTTC TTGCAGTTCA GTGCCATGAA GATGGCCCAC 
GCTCCGCCTG GGCACCACTC TGTGATCGGC CGTCCCAGTG TCAACGGCCT GGCCTACGCA 
GAGTATGTGA TCTATCGCGG AGAACAGGCC TACCCAGAGT ATCTCATCAC CTATCAGATA 
ATGAAGCCGG ACAGCACGCC TCAGTCCTCC ACAGGAGCGG AGCAGAAATC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171801.1
CDS	60..3893
Translation	MAVSRRSSQHQQSSLQPSPRNAVIASPPPESPSAIIPDSASLLSPSATGAAECGASTMESAAASSASASSPDRPAPVTAAAAGGPSVLGSSSCSSISSSATAGSQSPGSGAASPGEAVCGAGGAFRELFEACRNGDVSRVKRLVDSVNVNAKDMAGRKSTPLHFAAGFGRKDVVEHLLQTGANVHSRDDGGLIPLHNACSFGHAEVVSLLLCSGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLADPSAKTVLTGEYKKDELLEAARSGNEEKLMALLTPLNVNCHASDGRKSTPLHLAAGYNRVRIVQLLLQYGADVHAKDKGGLVPLHNACSYGHYEVTELLLKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSHGADPTLLNCHGKSAVDVAPTPELKERLTYEFKGHSLLQAAREADMAKVKKTAQEIISFKHPHSHDSALHCAVASPHPKRKQVTELLLRKGANIHEKNKDFMTPFHVAAERGHNDVLEVLQKHGAKVNAADTLGQTALHRAALAGHIQTCRLLLSYGADPSIVSLQGFTASQMGNEAVQQILNENVPPRNSDVDYRLLEAAKAGDLDTVKQLCSPQNVNCRDLEGRHSTPLHFAAGYNRVAVVEYLLHHGADVHAKDKGGLVPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKGKYEICKLLLKHGADPSKKNRDGNMALDMVKDGDTDIQDLLRGDAALLDAAKKGCLARVQKLCSPENINCRDTQGRNSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYGHVDIAALLIKFNTCVNATDKWAFTPLHEAAQKGRTQLCALLLAHGANPTMKNQEGQTALDLATADDIRALLMDAMPPDALPSCFKPQATVLSAALISPASTPSCLSAASSIDNLAGPLCDGASGGAAGPADGASSTDRKEGDGALIQGNKLTAVLDMNISQFLKSLGLEHLRDIFEREQITLDVLADMGHEELKEIGINAYGHRHKLIKGIERLLGGQQGANPYLTFHCTNQGTVLIDLAVDDKEFQSVEEEMQSTIREHRDGGNAGGVFSRYNVIKIQKVVNKKLRERYTHRQKEIADENHNHHNERMLFHGSPFINAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPTHKDRSCYLCHRQMLFCRVTLGKSFLQFSAMKMAHAPPGHHSVIGRPSVNGLAYAEYVIYRGEQAYPEYLITYQIMKPDSTPQSSTGAEQKS

Target ORF information:

RefSeq Version	XM_005171744.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase b (tnksb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171744.4

ORF Insert Sequence:

ATGGCGGTGT CCCGTCGATC CTCACAGCAC CAACAAAGCA GCCTACAACC GTCTCCGCGA 
AACGCCGTAA TCGCTTCCCC TCCTCCTGAA TCGCCGTCCG CTATTATTCC CGATTCCGCG 
AGTTTATTGA GCCCTTCAGC CACTGGCGCA GCAGAATGTG GCGCATCGAC GATGGAGTCC 
GCAGCGGCCT CCTCCGCCTC CGCCTCTTCC CCAGACCGGC CCGCCCCGGT AACAGCAGCG 
GCCGCGGGGG GACCGTCAGT CCTCGGCAGC TCCAGCTGTA GCAGCATTAG CTCCAGCGCG 
ACCGCGGGCA GCCAGAGCCC CGGATCCGGA GCCGCCAGTC CCGGGGAGGC GGTGTGCGGG 
GCTGGCGGGG CGTTCAGGGA GCTGTTCGAG GCCTGTCGGA ATGGAGATGT GTCGCGGGTG 
AAGAGGCTGG TGGACTCGGT GAATGTGAAC GCTAAAGACA TGGCCGGACG AAAATCAACC 
CCTCTGCATT TCGCTGCAGG CTTCGGCAGA AAGGATGTTG TCGAGCATCT CCTGCAAACC 
GGAGCCAACG TTCATTCCCG TGATGATGGT GGTCTGATCC CGCTCCATAA CGCTTGCTCA 
TTCGGCCACG CAGAAGTGGT CAGCCTGCTG TTGTGTAGCG GCGCCGACCC CAACGCTCGA 
GACAACTGGA ACTACACACC TCTGCATGAG GCCGCCATCA AAGGCAAAAT AGACGTCTGC 
ATCGTGTTGC TTCAGCACGG TGCTGATCCA AATATCAGAA ACACTGACGG CAAATCAGCT 
CTGGATCTGG CAGACCCTTC TGCTAAGACC GTCCTCACTG GTGAATATAA GAAGGATGAA 
CTATTGGAAG CAGCGAGGAG CGGAAATGAG GAAAAACTGA TGGCACTTTT GACCCCGCTT 
AACGTCAACT GTCATGCCAG TGATGGACGC AAGTCCACTC CTCTCCACCT GGCCGCTGGG 
TATAATCGTG TGCGTATCGT GCAGTTATTG CTGCAGTACG GTGCAGATGT CCATGCCAAA 
GACAAAGGTG GTTTGGTTCC TCTGCATAAT GCCTGTTCTT ATGGTCACTA TGAGGTCACA 
GAACTCCTGC TGAAGCATGG GGCGTGTGTT AATGCCATGG ACCTCTGGCA GTTCACGCCG 
CTGCACGAGG CGGCCAGTAA GAATCGAGTG GAGGTTTGTT CTCTGCTGCT GAGTCACGGC 
GCAGATCCAA CACTGCTGAA CTGCCACGGC AAGAGTGCGG TGGATGTGGC TCCCACACCT 
GAGCTGAAAG AGAGACTCAC CTATGAATTT AAAGGACACT CGCTTCTGCA AGCGGCCCGT 
GAGGCGGACA TGGCGAAGGT CAAGAAAACA GCTCAAGAAA TCATCAGCTT CAAACACCCG 
CACTCGCACG ACTCCGCTCT GCACTGCGCC GTAGCCTCTC CTCATCCCAA ACGCAAACAG 
GTCACAGAGT TACTGCTGAG GAAAGGTGCC AACATTCACG AGAAGAACAA GGATTTCATG 
ACCCCGTTTC ACGTAGCAGC TGAACGAGGT CATAATGATG TGCTTGAAGT CCTGCAGAAA 
CACGGAGCAA AGGTGAACGC TGCGGACACA CTGGGTCAGA CGGCTCTGCA CAGGGCTGCT 
CTTGCTGGTC ACATCCAGAC CTGCAGATTA CTGCTGAGTT ACGGAGCAGA TCCTTCAATC 
GTCTCACTGC AGGGCTTCAC TGCATCACAG ATGGGGAATG AAGCCGTTCA GCAAATACTC 
AATGAGAACG TTCCTCCCCG TAATTCAGAT GTGGATTACC GCTTGCTCGA AGCTGCTAAA 
GCTGGCGACC TTGATACAGT GAAGCAACTG TGCTCTCCTC AGAACGTGAA CTGTCGGGAT 
CTGGAGGGCC GTCACTCTAC ACCGCTACAC TTCGCCGCAG GCTACAACCG GGTGGCTGTG 
GTGGAGTATC TGCTCCATCA CGGGGCCGAT GTGCACGCTA AAGATAAAGG GGGTCTGGTT 
CCTCTGCACA ACGCATGCTC GTATGGTCAC TACGAGGTGG CGGAGCTGCT GGTGAGACAT 
GGAGCATCTG TGAACGTGGC CGATCTCTGG AAGTTCACAC CACTACATGA GGCTGCAGCA 
AAGGGCAAAT ACGAGATCTG CAAACTACTG CTCAAGCATG GTGCAGACCC GTCTAAGAAG 
AACCGTGATG GTAACATGGC TCTGGACATG GTGAAAGATG GAGACACGGA CATTCAAGAC 
CTGCTGCGTG GAGACGCCGC TCTGCTGGAC GCTGCCAAGA AGGGCTGTTT GGCCCGAGTG 
CAGAAACTCT GCAGCCCGGA GAACATCAAC TGCAGAGACA CACAGGGCAG GAACTCCACA 
CCGCTGCACC TTGCGGCTGG CTATAATAAC TTGGAGGTGG CCGAGTATCT CCTGGAGCAT 
GGAGCGGATG TGAACGCTCA GGATAAAGGA GGACTGATTC CTCTGCATAA TGCTGCTTCA 
TATGGGCATG TGGACATCGC CGCCCTGCTG ATCAAGTTCA ACACGTGTGT AAATGCGACA 
GATAAGTGGG CCTTCACTCC TTTGCATGAA GCTGCTCAGA AGGGCCGAAC ACAGCTGTGT 
GCACTGCTAC TGGCACATGG TGCAAACCCC ACCATGAAGA ATCAAGAGGG ACAGACTGCT 
CTGGACCTCG CCACGGCTGA TGACATCCGT GCTCTGCTGA TGGATGCGAT GCCCCCTGAT 
GCCCTGCCTA GCTGTTTCAA ACCGCAGGCC ACCGTGCTCA GCGCCGCGCT CATTTCTCCC 
GCCTCCACAC CCTCCTGCCT CTCCGCCGCC TCCAGCATCG ACAACCTGGC TGGACCTCTA 
TGTGACGGAG CCAGTGGAGG AGCCGCGGGA CCCGCTGATG GAGCCTCCAG CACTGATCGC 
AAAGAAGGAG ATGGTGCGTT AATACAAGGA AACAAACTAA CTGCTGTCCT GGATATGAAC 
ATCTCCCAGT TCCTGAAGAG TTTAGGCCTT GAACATTTGA GGGACATCTT TGAGAGAGAA 
CAGATCACCC TGGACGTATT GGCTGATATG GGTCATGAGG AACTCAAGGA GATAGGAATC 
AACGCTTACG GACACCGTCA TAAACTCATT AAAGGCATTG AGAGGCTGCT GGGAGGACAG 
CAGGGTGCCA ATCCATACCT GACGTTCCAC TGCACCAATC AGGGCACAGT ATTGATTGAC 
CTGGCTGTGG ATGATAAGGA GTTTCAGTCC GTTGAGGAAG AGATGCAAAG CACAATTCGG 
GAACACAGGG ATGGCGGAAA TGCAGGTGGC GTTTTCAGTC GTTACAATGT CATTAAGATC 
CAGAAGGTGG TGAATAAGAA ACTGAGAGAA CGATACACAC ACAGGCAGAA AGAGATCGCG 
GACGAGAATC ACAATCACCA CAATGAGCGA ATGCTGTTTC ACGGATCTCC GTTTATAAAC 
GCCATCATCC ATAAAGGCTT TGATGAGCGT CATGCGTATA TCGGCGGCAT GTTTGGAGCG 
GGTATATATT TTGCAGAGAA CTCTTCAAAG AGTAACCAGT ACGTCTACGG CATCGGTGGT 
GGTACAGGAT GCCCAACTCA CAAGGATCGT TCCTGTTACC TGTGCCACAG GCAAATGTTG 
TTCTGCCGTG TGACATTGGG AAAATCCTTC TTGCAGTTCA GTGCCATGAA GATGGCCCAC 
GCTCCGCCTG GGCACCACTC TGTGATCGGC CGTCCCAGTG TCAACGGCCT GGCCTACGCA 
GAGTATGTGA TCTATCGCGG AGAACAGGCC TACCCAGAGT ATCTCATCAC CTATCAGATA 
ATGAAGCCGG ACAGCACGCC TCAGTCCTCC ACAGGAGCGG AGCAGAAATC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnksb ( XM_005171745.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171802.1 Danio rerio tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase b (tnksb), transcript variant X3, mRNA.

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CloneID	ODa10085
Clone ID Related Accession (Same CDS sequence)	XM_005171745.3 , XM_005171745.4
Accession Version	XM_005171745.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tankyrase 1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005171745.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGT CCCGTCGATC CTCACAGCAC CAACAAAGCA GCCTACAACC GTCTCCGCGA 
AACGCCGTAA TCGCTTCCCC TCCTCCTGAA TCGCCGTCCG CTATTATTCC CGATTCCGCG 
AGTTTATTGA GCCCTTCAGC CACTGGCGCA GCAGAATGTG GCGCATCGAC GATGGAGTCC 
GCAGCGGCCT CCTCCGCCTC CGCCTCTTCC CCAGACCGGC CCGCCCCGGT AACAGCAGCG 
GCCGCGGGGG GACCGTCAGT CCTCGGCAGC TCCAGCTGTA GCAGCATTAG CTCCAGCGCG 
ACCGCGGGCA GCCAGAGCCC CGGATCCGGA GCCGCCAGTC CCGGGGAGGC GGTGTGCGGG 
GCTGGCGGGG CGTTCAGGGA GCTGTTCGAG GCCTGTCGGA ATGGAGATGT GTCGCGGGTG 
AAGAGGCTGG TGGACTCGGT GAATGTGAAC GCTAAAGACA TGGCCGGACG AAAATCAACC 
CCTCTGCATT TCGCTGCAGG CTTCGGCAGA AAGGATGTTG TCGAGCATCT CCTGCAAACC 
GGAGCCAACG TTCATTCCCG TGATGATGGT GGTCTGATCC CGCTCCATAA CGCTTGCTCA 
TTCGGCCACG CAGAAGTGGT CAGCCTGCTG TTGTGTAGCG GCGCCGACCC CAACGCTCGA 
GACAACTGGA ACTACACACC TCTGCATGAG GCCGCCATCA AAGGCAAAAT AGACGTCTGC 
ATCGTGTTGC TTCAGCACGG TGCTGATCCA AATATCAGAA ACACTGACGG CAAATCAGCT 
CTGGATCTGG CAGACCCTTC TGCTAAGACC GTCCTCACTG GTGAATATAA GAAGGATGAA 
CTATTGGAAG CAGCGAGGAG CGGAAATGAG GAAAAACTGA TGGCACTTTT GACCCCGCTT 
AACGTCAACT GTCATGCCAG TGATGGACGC AAGTCAACAT CACAAAAAAT GCTGTCCACT 
CCTCTCCACC TGGCCGCTGG GTATAATCGT GTGCGTATCG TGCAGTTATT GCTGCAGTAC 
GGTGCAGATG TCCATGCCAA AGACAAAGGT GGTTTGGTTC CTCTGCATAA TGCCTGTTCT 
TATGGTCACT ATGAGGTCAC AGAACTCCTG CTGAAGCATG GGGCGTGTGT TAATGCCATG 
GACCTCTGGC AGTTCACGCC GCTGCACGAG GCGGCCAGTA AGAATCGAGT GGAGGTTTGT 
TCTCTGCTGC TGAGTCACGG CGCAGATCCA ACACTGCTGA ACTGCCACGG CAAGAGTGCG 
GTGGATGTGG CTCCCACACC TGAGCTGAAA GAGAGACTCA CCTATGAATT TAAAGGACAC 
TCGCTTCTGC AAGCGGCCCG TGAGGCGGAC ATGGCGAAGG TCAAGAAAAC AGCTCAAGAA 
ATCATCAGCT TCAAACACCC GCACTCGCAC GACTCCGCTC TGCACTGCGC CGTAGCCTCT 
CCTCATCCCA AACGCAAACA GGTCACAGAG TTACTGCTGA GGAAAGGTGC CAACATTCAC 
GAGAAGAACA AGGATTTCAT GACCCCGTTT CACGTAGCAG CTGAACGAGG TCATAATGAT 
GTGCTTGAAG TCCTGCAGAA ACACGGAGCA AAGGTGAACG CTGCGGACAC ACTGGGTCAG 
ACGGCTCTGC ACAGGGCTGC TCTTGCTGGT CACATCCAGA CCTGCAGATT ACTGCTGAGT 
TACGGAGCAG ATCCTTCAAT CGTCTCACTG CAGGGCTTCA CTGCATCACA GATGGGGAAT 
GAAGCCGTTC AGCAAATACT CAATGAGAAC GTTCCTCCCC GTAATTCAGA TGTGGATTAC 
CGCTTGCTCG AAGCTGCTAA AGCTGGCGAC CTTGATACAG TGAAGCAACT GTGCTCTCCT 
CAGAACGTGA ACTGTCGGGA TCTGGAGGGC CGTCACTCTA CACCGCTACA CTTCGCCGCA 
GGCTACAACC GGGTGGCTGT GGTGGAGTAT CTGCTCCATC ACGGGGCCGA TGTGCACGCT 
AAAGATAAAG GGGGTCTGGT TCCTCTGCAC AACGCATGCT CGTATGGTCA CTACGAGGTG 
GCGGAGCTGC TGGTGAGACA TGGAGCATCT GTGAACGTGG CCGATCTCTG GAAGTTCACA 
CCACTACATG AGGCTGCAGC AAAGGGCAAA TACGAGATCT GCAAACTACT GCTCAAGCAT 
GGTGCAGACC CGTCTAAGAA GAACCGTGAT GGTAACATGG CTCTGGACAT GGTGAAAGAT 
GGAGACACGG ACATTCAAGA CCTGCTGCGT GGAGACGCCG CTCTGCTGGA CGCTGCCAAG 
AAGGGCTGTT TGGCCCGAGT GCAGAAACTC TGCAGCCCGG AGAACATCAA CTGCAGAGAC 
ACACAGGGCA GGAACTCCAC ACCGCTGCAC CTTGCGGCTG GCTATAATAA CTTGGAGGTG 
GCCGAGTATC TCCTGGAGCA TGGAGCGGAT GTGAACGCTC AGGATAAAGG AGGACTGATT 
CCTCTGCATA ATGCTGCTTC ATATGGGCAT GTGGACATCG CCGCCCTGCT GATCAAGTTC 
AACACGTGTG TAAATGCGAC AGATAAGTGG GCCTTCACTC CTTTGCATGA AGCTGCTCAG 
AAGGGCCGAA CACAGCTGTG TGCACTGCTA CTGGCACATG GTGCAAACCC CACCATGAAG 
AATCAAGAGG GACAGACTGC TCTGGACCTC GCCACGGCTG ATGACATCCG TGCTCTGCTG 
ATGGATGCGA TGCCCCCTGA TGCCCTGCCT AGCTGTTTCA AACCGCAGGC CACCGTGCTC 
AGCGCCGCGC TCATTTCTCC CGCCTCCACA CCCTCCTGCC TCTCCGCCGC CTCCAGCATC 
GACAACCTGG CTGGACCTCT ATGTGACGGA GCCAGTGGAG GAGCCGCGGG ACCCGCTGAT 
GGAGCCTCCA GCACTGATCG CAAAGAAGGA GATGCTGTCC TGGATATGAA CATCTCCCAG 
TTCCTGAAGA GTTTAGGCCT TGAACATTTG AGGGACATCT TTGAGAGAGA ACAGATCACC 
CTGGACGTAT TGGCTGATAT GGGTCATGAG GAACTCAAGG AGATAGGAAT CAACGCTTAC 
GGACACCGTC ATAAACTCAT TAAAGGCATT GAGAGGCTGC TGGGAGGACA GCAGGGTGCC 
AATCCATACC TGACGTTCCA CTGCACCAAT CAGGGCACAG TATTGATTGA CCTGGCTGTG 
GATGATAAGG AGTTTCAGTC CGTTGAGGAA GAGATGCAAA GCACAATTCG GGAACACAGG 
GATGGCGGAA ATGCAGGTGG CGTTTTCAGT CGTTACAATG TCATTAAGAT CCAGAAGGTG 
GTGAATAAGA AACTGAGAGA ACGATACACA CACAGGCAGA AAGAGATCGC GGACGAGAAT 
CACAATCACC ACAATGAGCG AATGCTGTTT CACGGATCTC CGTTTATAAA CGCCATCATC 
CATAAAGGCT TTGATGAGCG TCATGCGTAT ATCGGCGGCA TGTTTGGAGC GGGTATATAT 
TTTGCAGAGA ACTCTTCAAA GAGTAACCAG TACGTCTACG GCATCGGTGG TGGTACAGGA 
TGCCCAACTC ACAAGGATCG TTCCTGTTAC CTGTGCCACA GGCAAATGTT GTTCTGCCGT 
GTGACATTGG GAAAATCCTT CTTGCAGTTC AGTGCCATGA AGATGGCCCA CGCTCCGCCT 
GGGCACCACT CTGTGATCGG CCGTCCCAGT GTCAACGGCC TGGCCTACGC AGAGTATGTG 
ATCTATCGCG GAGAACAGGC CTACCCAGAG TATCTCATCA CCTATCAGAT AATGAAGCCG 
GACAGCACGC CTCAGTCCTC CACAGGAGCG GAGCAGAAAT CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171802.1
CDS	76..3900
Translation	MAVSRRSSQHQQSSLQPSPRNAVIASPPPESPSAIIPDSASLLSPSATGAAECGASTMESAAASSASASSPDRPAPVTAAAAGGPSVLGSSSCSSISSSATAGSQSPGSGAASPGEAVCGAGGAFRELFEACRNGDVSRVKRLVDSVNVNAKDMAGRKSTPLHFAAGFGRKDVVEHLLQTGANVHSRDDGGLIPLHNACSFGHAEVVSLLLCSGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLADPSAKTVLTGEYKKDELLEAARSGNEEKLMALLTPLNVNCHASDGRKSTSQKMLSTPLHLAAGYNRVRIVQLLLQYGADVHAKDKGGLVPLHNACSYGHYEVTELLLKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSHGADPTLLNCHGKSAVDVAPTPELKERLTYEFKGHSLLQAAREADMAKVKKTAQEIISFKHPHSHDSALHCAVASPHPKRKQVTELLLRKGANIHEKNKDFMTPFHVAAERGHNDVLEVLQKHGAKVNAADTLGQTALHRAALAGHIQTCRLLLSYGADPSIVSLQGFTASQMGNEAVQQILNENVPPRNSDVDYRLLEAAKAGDLDTVKQLCSPQNVNCRDLEGRHSTPLHFAAGYNRVAVVEYLLHHGADVHAKDKGGLVPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKGKYEICKLLLKHGADPSKKNRDGNMALDMVKDGDTDIQDLLRGDAALLDAAKKGCLARVQKLCSPENINCRDTQGRNSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYGHVDIAALLIKFNTCVNATDKWAFTPLHEAAQKGRTQLCALLLAHGANPTMKNQEGQTALDLATADDIRALLMDAMPPDALPSCFKPQATVLSAALISPASTPSCLSAASSIDNLAGPLCDGASGGAAGPADGASSTDRKEGDAVLDMNISQFLKSLGLEHLRDIFEREQITLDVLADMGHEELKEIGINAYGHRHKLIKGIERLLGGQQGANPYLTFHCTNQGTVLIDLAVDDKEFQSVEEEMQSTIREHRDGGNAGGVFSRYNVIKIQKVVNKKLRERYTHRQKEIADENHNHHNERMLFHGSPFINAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPTHKDRSCYLCHRQMLFCRVTLGKSFLQFSAMKMAHAPPGHHSVIGRPSVNGLAYAEYVIYRGEQAYPEYLITYQIMKPDSTPQSSTGAEQKS

Target ORF information:

RefSeq Version	XM_005171745.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase b (tnksb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171745.3

ORF Insert Sequence:

ATGGCGGTGT CCCGTCGATC CTCACAGCAC CAACAAAGCA GCCTACAACC GTCTCCGCGA 
AACGCCGTAA TCGCTTCCCC TCCTCCTGAA TCGCCGTCCG CTATTATTCC CGATTCCGCG 
AGTTTATTGA GCCCTTCAGC CACTGGCGCA GCAGAATGTG GCGCATCGAC GATGGAGTCC 
GCAGCGGCCT CCTCCGCCTC CGCCTCTTCC CCAGACCGGC CCGCCCCGGT AACAGCAGCG 
GCCGCGGGGG GACCGTCAGT CCTCGGCAGC TCCAGCTGTA GCAGCATTAG CTCCAGCGCG 
ACCGCGGGCA GCCAGAGCCC CGGATCCGGA GCCGCCAGTC CCGGGGAGGC GGTGTGCGGG 
GCTGGCGGGG CGTTCAGGGA GCTGTTCGAG GCCTGTCGGA ATGGAGATGT GTCGCGGGTG 
AAGAGGCTGG TGGACTCGGT GAATGTGAAC GCTAAAGACA TGGCCGGACG AAAATCAACC 
CCTCTGCATT TCGCTGCAGG CTTCGGCAGA AAGGATGTTG TCGAGCATCT CCTGCAAACC 
GGAGCCAACG TTCATTCCCG TGATGATGGT GGTCTGATCC CGCTCCATAA CGCTTGCTCA 
TTCGGCCACG CAGAAGTGGT CAGCCTGCTG TTGTGTAGCG GCGCCGACCC CAACGCTCGA 
GACAACTGGA ACTACACACC TCTGCATGAG GCCGCCATCA AAGGCAAAAT AGACGTCTGC 
ATCGTGTTGC TTCAGCACGG TGCTGATCCA AATATCAGAA ACACTGACGG CAAATCAGCT 
CTGGATCTGG CAGACCCTTC TGCTAAGACC GTCCTCACTG GTGAATATAA GAAGGATGAA 
CTATTGGAAG CAGCGAGGAG CGGAAATGAG GAAAAACTGA TGGCACTTTT GACCCCGCTT 
AACGTCAACT GTCATGCCAG TGATGGACGC AAGTCAACAT CACAAAAAAT GCTGTCCACT 
CCTCTCCACC TGGCCGCTGG GTATAATCGT GTGCGTATCG TGCAGTTATT GCTGCAGTAC 
GGTGCAGATG TCCATGCCAA AGACAAAGGT GGTTTGGTTC CTCTGCATAA TGCCTGTTCT 
TATGGTCACT ATGAGGTCAC AGAACTCCTG CTGAAGCATG GGGCGTGTGT TAATGCCATG 
GACCTCTGGC AGTTCACGCC GCTGCACGAG GCGGCCAGTA AGAATCGAGT GGAGGTTTGT 
TCTCTGCTGC TGAGTCACGG CGCAGATCCA ACACTGCTGA ACTGCCACGG CAAGAGTGCG 
GTGGATGTGG CTCCCACACC TGAGCTGAAA GAGAGACTCA CCTATGAATT TAAAGGACAC 
TCGCTTCTGC AAGCGGCCCG TGAGGCGGAC ATGGCGAAGG TCAAGAAAAC AGCTCAAGAA 
ATCATCAGCT TCAAACACCC GCACTCGCAC GACTCCGCTC TGCACTGCGC CGTAGCCTCT 
CCTCATCCCA AACGCAAACA GGTCACAGAG TTACTGCTGA GGAAAGGTGC CAACATTCAC 
GAGAAGAACA AGGATTTCAT GACCCCGTTT CACGTAGCAG CTGAACGAGG TCATAATGAT 
GTGCTTGAAG TCCTGCAGAA ACACGGAGCA AAGGTGAACG CTGCGGACAC ACTGGGTCAG 
ACGGCTCTGC ACAGGGCTGC TCTTGCTGGT CACATCCAGA CCTGCAGATT ACTGCTGAGT 
TACGGAGCAG ATCCTTCAAT CGTCTCACTG CAGGGCTTCA CTGCATCACA GATGGGGAAT 
GAAGCCGTTC AGCAAATACT CAATGAGAAC GTTCCTCCCC GTAATTCAGA TGTGGATTAC 
CGCTTGCTCG AAGCTGCTAA AGCTGGCGAC CTTGATACAG TGAAGCAACT GTGCTCTCCT 
CAGAACGTGA ACTGTCGGGA TCTGGAGGGC CGTCACTCTA CACCGCTACA CTTCGCCGCA 
GGCTACAACC GGGTGGCTGT GGTGGAGTAT CTGCTCCATC ACGGGGCCGA TGTGCACGCT 
AAAGATAAAG GGGGTCTGGT TCCTCTGCAC AACGCATGCT CGTATGGTCA CTACGAGGTG 
GCGGAGCTGC TGGTGAGACA TGGAGCATCT GTGAACGTGG CCGATCTCTG GAAGTTCACA 
CCACTACATG AGGCTGCAGC AAAGGGCAAA TACGAGATCT GCAAACTACT GCTCAAGCAT 
GGTGCAGACC CGTCTAAGAA GAACCGTGAT GGTAACATGG CTCTGGACAT GGTGAAAGAT 
GGAGACACGG ACATTCAAGA CCTGCTGCGT GGAGACGCCG CTCTGCTGGA CGCTGCCAAG 
AAGGGCTGTT TGGCCCGAGT GCAGAAACTC TGCAGCCCGG AGAACATCAA CTGCAGAGAC 
ACACAGGGCA GGAACTCCAC ACCGCTGCAC CTTGCGGCTG GCTATAATAA CTTGGAGGTG 
GCCGAGTATC TCCTGGAGCA TGGAGCGGAT GTGAACGCTC AGGATAAAGG AGGACTGATT 
CCTCTGCATA ATGCTGCTTC ATATGGGCAT GTGGACATCG CCGCCCTGCT GATCAAGTTC 
AACACGTGTG TAAATGCGAC AGATAAGTGG GCCTTCACTC CTTTGCATGA AGCTGCTCAG 
AAGGGCCGAA CACAGCTGTG TGCACTGCTA CTGGCACATG GTGCAAACCC CACCATGAAG 
AATCAAGAGG GACAGACTGC TCTGGACCTC GCCACGGCTG ATGACATCCG TGCTCTGCTG 
ATGGATGCGA TGCCCCCTGA TGCCCTGCCT AGCTGTTTCA AACCGCAGGC CACCGTGCTC 
AGCGCCGCGC TCATTTCTCC CGCCTCCACA CCCTCCTGCC TCTCCGCCGC CTCCAGCATC 
GACAACCTGG CTGGACCTCT ATGTGACGGA GCCAGTGGAG GAGCCGCGGG ACCCGCTGAT 
GGAGCCTCCA GCACTGATCG CAAAGAAGGA GATGCTGTCC TGGATATGAA CATCTCCCAG 
TTCCTGAAGA GTTTAGGCCT TGAACATTTG AGGGACATCT TTGAGAGAGA ACAGATCACC 
CTGGACGTAT TGGCTGATAT GGGTCATGAG GAACTCAAGG AGATAGGAAT CAACGCTTAC 
GGACACCGTC ATAAACTCAT TAAAGGCATT GAGAGGCTGC TGGGAGGACA GCAGGGTGCC 
AATCCATACC TGACGTTCCA CTGCACCAAT CAGGGCACAG TATTGATTGA CCTGGCTGTG 
GATGATAAGG AGTTTCAGTC CGTTGAGGAA GAGATGCAAA GCACAATTCG GGAACACAGG 
GATGGCGGAA ATGCAGGTGG CGTTTTCAGT CGTTACAATG TCATTAAGAT CCAGAAGGTG 
GTGAATAAGA AACTGAGAGA ACGATACACA CACAGGCAGA AAGAGATCGC GGACGAGAAT 
CACAATCACC ACAATGAGCG AATGCTGTTT CACGGATCTC CGTTTATAAA CGCCATCATC 
CATAAAGGCT TTGATGAGCG TCATGCGTAT ATCGGCGGCA TGTTTGGAGC GGGTATATAT 
TTTGCAGAGA ACTCTTCAAA GAGTAACCAG TACGTCTACG GCATCGGTGG TGGTACAGGA 
TGCCCAACTC ACAAGGATCG TTCCTGTTAC CTGTGCCACA GGCAAATGTT GTTCTGCCGT 
GTGACATTGG GAAAATCCTT CTTGCAGTTC AGTGCCATGA AGATGGCCCA CGCTCCGCCT 
GGGCACCACT CTGTGATCGG CCGTCCCAGT GTCAACGGCC TGGCCTACGC AGAGTATGTG 
ATCTATCGCG GAGAACAGGC CTACCCAGAG TATCTCATCA CCTATCAGAT AATGAAGCCG 
GACAGCACGC CTCAGTCCTC CACAGGAGCG GAGCAGAAAT CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnksb ( XM_005171745.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171802.1 Danio rerio tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase b (tnksb), transcript variant X3, mRNA.

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CloneID	ODa10085
Clone ID Related Accession (Same CDS sequence)	XM_005171745.3 , XM_005171745.4
Accession Version	XM_005171745.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tankyrase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005171745.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGT CCCGTCGATC CTCACAGCAC CAACAAAGCA GCCTACAACC GTCTCCGCGA 
AACGCCGTAA TCGCTTCCCC TCCTCCTGAA TCGCCGTCCG CTATTATTCC CGATTCCGCG 
AGTTTATTGA GCCCTTCAGC CACTGGCGCA GCAGAATGTG GCGCATCGAC GATGGAGTCC 
GCAGCGGCCT CCTCCGCCTC CGCCTCTTCC CCAGACCGGC CCGCCCCGGT AACAGCAGCG 
GCCGCGGGGG GACCGTCAGT CCTCGGCAGC TCCAGCTGTA GCAGCATTAG CTCCAGCGCG 
ACCGCGGGCA GCCAGAGCCC CGGATCCGGA GCCGCCAGTC CCGGGGAGGC GGTGTGCGGG 
GCTGGCGGGG CGTTCAGGGA GCTGTTCGAG GCCTGTCGGA ATGGAGATGT GTCGCGGGTG 
AAGAGGCTGG TGGACTCGGT GAATGTGAAC GCTAAAGACA TGGCCGGACG AAAATCAACC 
CCTCTGCATT TCGCTGCAGG CTTCGGCAGA AAGGATGTTG TCGAGCATCT CCTGCAAACC 
GGAGCCAACG TTCATTCCCG TGATGATGGT GGTCTGATCC CGCTCCATAA CGCTTGCTCA 
TTCGGCCACG CAGAAGTGGT CAGCCTGCTG TTGTGTAGCG GCGCCGACCC CAACGCTCGA 
GACAACTGGA ACTACACACC TCTGCATGAG GCCGCCATCA AAGGCAAAAT AGACGTCTGC 
ATCGTGTTGC TTCAGCACGG TGCTGATCCA AATATCAGAA ACACTGACGG CAAATCAGCT 
CTGGATCTGG CAGACCCTTC TGCTAAGACC GTCCTCACTG GTGAATATAA GAAGGATGAA 
CTATTGGAAG CAGCGAGGAG CGGAAATGAG GAAAAACTGA TGGCACTTTT GACCCCGCTT 
AACGTCAACT GTCATGCCAG TGATGGACGC AAGTCAACAT CACAAAAAAT GCTGTCCACT 
CCTCTCCACC TGGCCGCTGG GTATAATCGT GTGCGTATCG TGCAGTTATT GCTGCAGTAC 
GGTGCAGATG TCCATGCCAA AGACAAAGGT GGTTTGGTTC CTCTGCATAA TGCCTGTTCT 
TATGGTCACT ATGAGGTCAC AGAACTCCTG CTGAAGCATG GGGCGTGTGT TAATGCCATG 
GACCTCTGGC AGTTCACGCC GCTGCACGAG GCGGCCAGTA AGAATCGAGT GGAGGTTTGT 
TCTCTGCTGC TGAGTCACGG CGCAGATCCA ACACTGCTGA ACTGCCACGG CAAGAGTGCG 
GTGGATGTGG CTCCCACACC TGAGCTGAAA GAGAGACTCA CCTATGAATT TAAAGGACAC 
TCGCTTCTGC AAGCGGCCCG TGAGGCGGAC ATGGCGAAGG TCAAGAAAAC AGCTCAAGAA 
ATCATCAGCT TCAAACACCC GCACTCGCAC GACTCCGCTC TGCACTGCGC CGTAGCCTCT 
CCTCATCCCA AACGCAAACA GGTCACAGAG TTACTGCTGA GGAAAGGTGC CAACATTCAC 
GAGAAGAACA AGGATTTCAT GACCCCGTTT CACGTAGCAG CTGAACGAGG TCATAATGAT 
GTGCTTGAAG TCCTGCAGAA ACACGGAGCA AAGGTGAACG CTGCGGACAC ACTGGGTCAG 
ACGGCTCTGC ACAGGGCTGC TCTTGCTGGT CACATCCAGA CCTGCAGATT ACTGCTGAGT 
TACGGAGCAG ATCCTTCAAT CGTCTCACTG CAGGGCTTCA CTGCATCACA GATGGGGAAT 
GAAGCCGTTC AGCAAATACT CAATGAGAAC GTTCCTCCCC GTAATTCAGA TGTGGATTAC 
CGCTTGCTCG AAGCTGCTAA AGCTGGCGAC CTTGATACAG TGAAGCAACT GTGCTCTCCT 
CAGAACGTGA ACTGTCGGGA TCTGGAGGGC CGTCACTCTA CACCGCTACA CTTCGCCGCA 
GGCTACAACC GGGTGGCTGT GGTGGAGTAT CTGCTCCATC ACGGGGCCGA TGTGCACGCT 
AAAGATAAAG GGGGTCTGGT TCCTCTGCAC AACGCATGCT CGTATGGTCA CTACGAGGTG 
GCGGAGCTGC TGGTGAGACA TGGAGCATCT GTGAACGTGG CCGATCTCTG GAAGTTCACA 
CCACTACATG AGGCTGCAGC AAAGGGCAAA TACGAGATCT GCAAACTACT GCTCAAGCAT 
GGTGCAGACC CGTCTAAGAA GAACCGTGAT GGTAACATGG CTCTGGACAT GGTGAAAGAT 
GGAGACACGG ACATTCAAGA CCTGCTGCGT GGAGACGCCG CTCTGCTGGA CGCTGCCAAG 
AAGGGCTGTT TGGCCCGAGT GCAGAAACTC TGCAGCCCGG AGAACATCAA CTGCAGAGAC 
ACACAGGGCA GGAACTCCAC ACCGCTGCAC CTTGCGGCTG GCTATAATAA CTTGGAGGTG 
GCCGAGTATC TCCTGGAGCA TGGAGCGGAT GTGAACGCTC AGGATAAAGG AGGACTGATT 
CCTCTGCATA ATGCTGCTTC ATATGGGCAT GTGGACATCG CCGCCCTGCT GATCAAGTTC 
AACACGTGTG TAAATGCGAC AGATAAGTGG GCCTTCACTC CTTTGCATGA AGCTGCTCAG 
AAGGGCCGAA CACAGCTGTG TGCACTGCTA CTGGCACATG GTGCAAACCC CACCATGAAG 
AATCAAGAGG GACAGACTGC TCTGGACCTC GCCACGGCTG ATGACATCCG TGCTCTGCTG 
ATGGATGCGA TGCCCCCTGA TGCCCTGCCT AGCTGTTTCA AACCGCAGGC CACCGTGCTC 
AGCGCCGCGC TCATTTCTCC CGCCTCCACA CCCTCCTGCC TCTCCGCCGC CTCCAGCATC 
GACAACCTGG CTGGACCTCT ATGTGACGGA GCCAGTGGAG GAGCCGCGGG ACCCGCTGAT 
GGAGCCTCCA GCACTGATCG CAAAGAAGGA GATGCTGTCC TGGATATGAA CATCTCCCAG 
TTCCTGAAGA GTTTAGGCCT TGAACATTTG AGGGACATCT TTGAGAGAGA ACAGATCACC 
CTGGACGTAT TGGCTGATAT GGGTCATGAG GAACTCAAGG AGATAGGAAT CAACGCTTAC 
GGACACCGTC ATAAACTCAT TAAAGGCATT GAGAGGCTGC TGGGAGGACA GCAGGGTGCC 
AATCCATACC TGACGTTCCA CTGCACCAAT CAGGGCACAG TATTGATTGA CCTGGCTGTG 
GATGATAAGG AGTTTCAGTC CGTTGAGGAA GAGATGCAAA GCACAATTCG GGAACACAGG 
GATGGCGGAA ATGCAGGTGG CGTTTTCAGT CGTTACAATG TCATTAAGAT CCAGAAGGTG 
GTGAATAAGA AACTGAGAGA ACGATACACA CACAGGCAGA AAGAGATCGC GGACGAGAAT 
CACAATCACC ACAATGAGCG AATGCTGTTT CACGGATCTC CGTTTATAAA CGCCATCATC 
CATAAAGGCT TTGATGAGCG TCATGCGTAT ATCGGCGGCA TGTTTGGAGC GGGTATATAT 
TTTGCAGAGA ACTCTTCAAA GAGTAACCAG TACGTCTACG GCATCGGTGG TGGTACAGGA 
TGCCCAACTC ACAAGGATCG TTCCTGTTAC CTGTGCCACA GGCAAATGTT GTTCTGCCGT 
GTGACATTGG GAAAATCCTT CTTGCAGTTC AGTGCCATGA AGATGGCCCA CGCTCCGCCT 
GGGCACCACT CTGTGATCGG CCGTCCCAGT GTCAACGGCC TGGCCTACGC AGAGTATGTG 
ATCTATCGCG GAGAACAGGC CTACCCAGAG TATCTCATCA CCTATCAGAT AATGAAGCCG 
GACAGCACGC CTCAGTCCTC CACAGGAGCG GAGCAGAAAT CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171802.1
CDS	59..3883
Translation	MAVSRRSSQHQQSSLQPSPRNAVIASPPPESPSAIIPDSASLLSPSATGAAECGASTMESAAASSASASSPDRPAPVTAAAAGGPSVLGSSSCSSISSSATAGSQSPGSGAASPGEAVCGAGGAFRELFEACRNGDVSRVKRLVDSVNVNAKDMAGRKSTPLHFAAGFGRKDVVEHLLQTGANVHSRDDGGLIPLHNACSFGHAEVVSLLLCSGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLADPSAKTVLTGEYKKDELLEAARSGNEEKLMALLTPLNVNCHASDGRKSTSQKMLSTPLHLAAGYNRVRIVQLLLQYGADVHAKDKGGLVPLHNACSYGHYEVTELLLKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSHGADPTLLNCHGKSAVDVAPTPELKERLTYEFKGHSLLQAAREADMAKVKKTAQEIISFKHPHSHDSALHCAVASPHPKRKQVTELLLRKGANIHEKNKDFMTPFHVAAERGHNDVLEVLQKHGAKVNAADTLGQTALHRAALAGHIQTCRLLLSYGADPSIVSLQGFTASQMGNEAVQQILNENVPPRNSDVDYRLLEAAKAGDLDTVKQLCSPQNVNCRDLEGRHSTPLHFAAGYNRVAVVEYLLHHGADVHAKDKGGLVPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKGKYEICKLLLKHGADPSKKNRDGNMALDMVKDGDTDIQDLLRGDAALLDAAKKGCLARVQKLCSPENINCRDTQGRNSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYGHVDIAALLIKFNTCVNATDKWAFTPLHEAAQKGRTQLCALLLAHGANPTMKNQEGQTALDLATADDIRALLMDAMPPDALPSCFKPQATVLSAALISPASTPSCLSAASSIDNLAGPLCDGASGGAAGPADGASSTDRKEGDAVLDMNISQFLKSLGLEHLRDIFEREQITLDVLADMGHEELKEIGINAYGHRHKLIKGIERLLGGQQGANPYLTFHCTNQGTVLIDLAVDDKEFQSVEEEMQSTIREHRDGGNAGGVFSRYNVIKIQKVVNKKLRERYTHRQKEIADENHNHHNERMLFHGSPFINAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPTHKDRSCYLCHRQMLFCRVTLGKSFLQFSAMKMAHAPPGHHSVIGRPSVNGLAYAEYVIYRGEQAYPEYLITYQIMKPDSTPQSSTGAEQKS

Target ORF information:

RefSeq Version	XM_005171745.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase b (tnksb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171745.4

ORF Insert Sequence:

ATGGCGGTGT CCCGTCGATC CTCACAGCAC CAACAAAGCA GCCTACAACC GTCTCCGCGA 
AACGCCGTAA TCGCTTCCCC TCCTCCTGAA TCGCCGTCCG CTATTATTCC CGATTCCGCG 
AGTTTATTGA GCCCTTCAGC CACTGGCGCA GCAGAATGTG GCGCATCGAC GATGGAGTCC 
GCAGCGGCCT CCTCCGCCTC CGCCTCTTCC CCAGACCGGC CCGCCCCGGT AACAGCAGCG 
GCCGCGGGGG GACCGTCAGT CCTCGGCAGC TCCAGCTGTA GCAGCATTAG CTCCAGCGCG 
ACCGCGGGCA GCCAGAGCCC CGGATCCGGA GCCGCCAGTC CCGGGGAGGC GGTGTGCGGG 
GCTGGCGGGG CGTTCAGGGA GCTGTTCGAG GCCTGTCGGA ATGGAGATGT GTCGCGGGTG 
AAGAGGCTGG TGGACTCGGT GAATGTGAAC GCTAAAGACA TGGCCGGACG AAAATCAACC 
CCTCTGCATT TCGCTGCAGG CTTCGGCAGA AAGGATGTTG TCGAGCATCT CCTGCAAACC 
GGAGCCAACG TTCATTCCCG TGATGATGGT GGTCTGATCC CGCTCCATAA CGCTTGCTCA 
TTCGGCCACG CAGAAGTGGT CAGCCTGCTG TTGTGTAGCG GCGCCGACCC CAACGCTCGA 
GACAACTGGA ACTACACACC TCTGCATGAG GCCGCCATCA AAGGCAAAAT AGACGTCTGC 
ATCGTGTTGC TTCAGCACGG TGCTGATCCA AATATCAGAA ACACTGACGG CAAATCAGCT 
CTGGATCTGG CAGACCCTTC TGCTAAGACC GTCCTCACTG GTGAATATAA GAAGGATGAA 
CTATTGGAAG CAGCGAGGAG CGGAAATGAG GAAAAACTGA TGGCACTTTT GACCCCGCTT 
AACGTCAACT GTCATGCCAG TGATGGACGC AAGTCAACAT CACAAAAAAT GCTGTCCACT 
CCTCTCCACC TGGCCGCTGG GTATAATCGT GTGCGTATCG TGCAGTTATT GCTGCAGTAC 
GGTGCAGATG TCCATGCCAA AGACAAAGGT GGTTTGGTTC CTCTGCATAA TGCCTGTTCT 
TATGGTCACT ATGAGGTCAC AGAACTCCTG CTGAAGCATG GGGCGTGTGT TAATGCCATG 
GACCTCTGGC AGTTCACGCC GCTGCACGAG GCGGCCAGTA AGAATCGAGT GGAGGTTTGT 
TCTCTGCTGC TGAGTCACGG CGCAGATCCA ACACTGCTGA ACTGCCACGG CAAGAGTGCG 
GTGGATGTGG CTCCCACACC TGAGCTGAAA GAGAGACTCA CCTATGAATT TAAAGGACAC 
TCGCTTCTGC AAGCGGCCCG TGAGGCGGAC ATGGCGAAGG TCAAGAAAAC AGCTCAAGAA 
ATCATCAGCT TCAAACACCC GCACTCGCAC GACTCCGCTC TGCACTGCGC CGTAGCCTCT 
CCTCATCCCA AACGCAAACA GGTCACAGAG TTACTGCTGA GGAAAGGTGC CAACATTCAC 
GAGAAGAACA AGGATTTCAT GACCCCGTTT CACGTAGCAG CTGAACGAGG TCATAATGAT 
GTGCTTGAAG TCCTGCAGAA ACACGGAGCA AAGGTGAACG CTGCGGACAC ACTGGGTCAG 
ACGGCTCTGC ACAGGGCTGC TCTTGCTGGT CACATCCAGA CCTGCAGATT ACTGCTGAGT 
TACGGAGCAG ATCCTTCAAT CGTCTCACTG CAGGGCTTCA CTGCATCACA GATGGGGAAT 
GAAGCCGTTC AGCAAATACT CAATGAGAAC GTTCCTCCCC GTAATTCAGA TGTGGATTAC 
CGCTTGCTCG AAGCTGCTAA AGCTGGCGAC CTTGATACAG TGAAGCAACT GTGCTCTCCT 
CAGAACGTGA ACTGTCGGGA TCTGGAGGGC CGTCACTCTA CACCGCTACA CTTCGCCGCA 
GGCTACAACC GGGTGGCTGT GGTGGAGTAT CTGCTCCATC ACGGGGCCGA TGTGCACGCT 
AAAGATAAAG GGGGTCTGGT TCCTCTGCAC AACGCATGCT CGTATGGTCA CTACGAGGTG 
GCGGAGCTGC TGGTGAGACA TGGAGCATCT GTGAACGTGG CCGATCTCTG GAAGTTCACA 
CCACTACATG AGGCTGCAGC AAAGGGCAAA TACGAGATCT GCAAACTACT GCTCAAGCAT 
GGTGCAGACC CGTCTAAGAA GAACCGTGAT GGTAACATGG CTCTGGACAT GGTGAAAGAT 
GGAGACACGG ACATTCAAGA CCTGCTGCGT GGAGACGCCG CTCTGCTGGA CGCTGCCAAG 
AAGGGCTGTT TGGCCCGAGT GCAGAAACTC TGCAGCCCGG AGAACATCAA CTGCAGAGAC 
ACACAGGGCA GGAACTCCAC ACCGCTGCAC CTTGCGGCTG GCTATAATAA CTTGGAGGTG 
GCCGAGTATC TCCTGGAGCA TGGAGCGGAT GTGAACGCTC AGGATAAAGG AGGACTGATT 
CCTCTGCATA ATGCTGCTTC ATATGGGCAT GTGGACATCG CCGCCCTGCT GATCAAGTTC 
AACACGTGTG TAAATGCGAC AGATAAGTGG GCCTTCACTC CTTTGCATGA AGCTGCTCAG 
AAGGGCCGAA CACAGCTGTG TGCACTGCTA CTGGCACATG GTGCAAACCC CACCATGAAG 
AATCAAGAGG GACAGACTGC TCTGGACCTC GCCACGGCTG ATGACATCCG TGCTCTGCTG 
ATGGATGCGA TGCCCCCTGA TGCCCTGCCT AGCTGTTTCA AACCGCAGGC CACCGTGCTC 
AGCGCCGCGC TCATTTCTCC CGCCTCCACA CCCTCCTGCC TCTCCGCCGC CTCCAGCATC 
GACAACCTGG CTGGACCTCT ATGTGACGGA GCCAGTGGAG GAGCCGCGGG ACCCGCTGAT 
GGAGCCTCCA GCACTGATCG CAAAGAAGGA GATGCTGTCC TGGATATGAA CATCTCCCAG 
TTCCTGAAGA GTTTAGGCCT TGAACATTTG AGGGACATCT TTGAGAGAGA ACAGATCACC 
CTGGACGTAT TGGCTGATAT GGGTCATGAG GAACTCAAGG AGATAGGAAT CAACGCTTAC 
GGACACCGTC ATAAACTCAT TAAAGGCATT GAGAGGCTGC TGGGAGGACA GCAGGGTGCC 
AATCCATACC TGACGTTCCA CTGCACCAAT CAGGGCACAG TATTGATTGA CCTGGCTGTG 
GATGATAAGG AGTTTCAGTC CGTTGAGGAA GAGATGCAAA GCACAATTCG GGAACACAGG 
GATGGCGGAA ATGCAGGTGG CGTTTTCAGT CGTTACAATG TCATTAAGAT CCAGAAGGTG 
GTGAATAAGA AACTGAGAGA ACGATACACA CACAGGCAGA AAGAGATCGC GGACGAGAAT 
CACAATCACC ACAATGAGCG AATGCTGTTT CACGGATCTC CGTTTATAAA CGCCATCATC 
CATAAAGGCT TTGATGAGCG TCATGCGTAT ATCGGCGGCA TGTTTGGAGC GGGTATATAT 
TTTGCAGAGA ACTCTTCAAA GAGTAACCAG TACGTCTACG GCATCGGTGG TGGTACAGGA 
TGCCCAACTC ACAAGGATCG TTCCTGTTAC CTGTGCCACA GGCAAATGTT GTTCTGCCGT 
GTGACATTGG GAAAATCCTT CTTGCAGTTC AGTGCCATGA AGATGGCCCA CGCTCCGCCT 
GGGCACCACT CTGTGATCGG CCGTCCCAGT GTCAACGGCC TGGCCTACGC AGAGTATGTG 
ATCTATCGCG GAGAACAGGC CTACCCAGAG TATCTCATCA CCTATCAGAT AATGAAGCCG 
GACAGCACGC CTCAGTCCTC CACAGGAGCG GAGCAGAAAT CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Tankyrase inhibition aggravates kidney injury in the absence of CD2AP.
Cell death & disease7e2302(2016 Jul)
Kuusela S,Wang H,Wasik AA,Suleiman H,Lehtonen S

Tankyrases regulate glucoregulatory mechanisms and somatic growth via the central melanocortin system in zebrafish larvae.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology29(11)4435-48(2015 Nov)
Wang H,Semenova S,Kuusela S,Panula P,Lehtonen S

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

RNA-Seq analysis in mutant zebrafish reveals role of U1C protein in alternative splicing regulation.
The EMBO journal30(10)1965-76(2011 May)
R?sel TD,Hung LH,Medenbach J,Donde K,Starke S,Benes V,R?tsch G,Bindereif A

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tnksb cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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