WFS1A cDNA ORF clone, Danio rerio(Zebrafish)

The following WFS1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WFS1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa05550 XM_690160.9
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Danio rerio Wolfram syndrome 1a (wolframin) (wfs1a), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
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ODa05550 XM_690160.8 Danio rerio Wolfram syndrome 1a (wolframin) (wfs1a), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa05550
    Clone ID Related Accession (Same CDS sequence) XM_690160.9 , XM_690160.8
    Accession Version XM_690160.9 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3186bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product wolframin
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_690160.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATCTTA ATGCCAACCC ACCATCATCT CCTGAGACAT CCAAGGAGAA AAGTGCAACC 
    CATAAACGTC TCAACCCTCC CGTTCCAGCC ACCAAATCCA TTCCAGGCAC AGAGACGGCC
    GGTCCTCCTC TCTCCAAATC AGGCAGCCTA GAAAAACCAT CCCCTTCTTC AACTCTGGAT
    ACTAAATTAA AACACGGCAG TGTTAAAACC TCAAAAGTTC CAGCTGAACC AACACCTGAG
    AAACAACCCA CCCCTGCATC ATCACATATC ATCTCTAAAC CTGTTCCATC TATGCAAGCC
    CCAACATCAT CAAAAACAAC ACAGCCAATA TTATCTTCTC CCAATGAACC ATCCGCACCA
    AGCATTGCCA TTTCCGCTGA CCCACCCCCA CCTACAGCTC CTCCTGAGGG CTTAACGTCC
    ACCACCAAAA CACCTGCATC TGACTCCGGT AAACTTCCCC GCCAGCGGAG CTTTTTGGCT
    GCGGCACAAA AGGTGATGAT GCAGGAGAAA ACAAGAAAGG CTGAGGAACA GGCTAAAGCT
    GAAGAGTGTG ATGATATGGA AGACGATCTG CCTTTTGAGG AGCTCCAGAA AAAAGCTGAA
    GCAGGAGACC CTAGAGCACA GAGTCGACTC GGTAGATACT ACCTTAAACT GGCAGAAGAA
    AAAGACGCAG AGAAAAATAA CCTCACAGCT GTCGAATGGT TGATGAAGGC TGCAAAACAA
    GGCCGAAGGG ATGCAGCTAA ATTACTTCAG AAATGCTGGA TGCAAAAGAA AGGTATTACA
    GCAGAGAACG CACAGGAGGT GAGATGTCTG TCTTCAGAGA GTAAGTTTGA GCAGGCAGTG
    AGGAGAGCAG CCATGACCAT GTACTGGAAA CTCAATCCCG ACAGAAAGAA GAAAGTAGCC
    ATGTCTGAGA TGCTGGAGAA TGTCAGCCAG GTCAATACTG TACCAGGTGA AGCTTCTGTA
    TGTGGTGTAG CGCCCAGCAT TCAAACTCAG AGGAAAATCC TGGAGACAAT GGTGTCCAAT
    GAATCTAGCT CTAAATATGT GGATGTGGAA GATTTTGTGG AGATGACAAA AAAGTTCACA
    GAAGGCATTT CCCGTACTCC CACACTAAGC CCCTCTGGTG TATATGCAAA TGAAGAGGAG
    GGGATATTAC ATAAAAGCGA TGTAGCCAGA TTTGATAAGG AAAAAAACGA AATGGCTCCT
    GTTACTCATA AGCGCCAACG AAAGTCTAGC TGGGGAATGA GGAGCAGTGG GTTGATGCTG
    ACTACCAGAC GCAGCGATGC CATGAGGAGA GCCCTGGACA TAAAAGCTCA CTTTTTGGGT
    CTGCAATATC CTCTTCATGC CATTATTGAT ATGAAGGAAC ATTTAATTGA CTGGGCGTCT
    CGTGCTGGTG TCCAGTGGCT GAGCACTATT ATCCCGACAC AACATGTCAA TGCCCTTATC
    TTCTTCTTCA TCATCTCCAA TCTCACCATC GATTTGTTTG CCTTCGCCAT CCCGCTCCTC
    ATCTTCTACC TGTCCTTTAT TTCCATGGCC ATCTGCACAC TGAGAGTCTT CCAGAGCAGC
    AAAGGTTGGG AGAACTTCCG TGCCTTTACC GCTCTCCTCA CACACTTTGA GCCCAGCCTG
    GATGTAGAGC AAGCGGAGAA CAACTTTGGC TGGAACAACC TGGAGCAGTA CCTCTACTTC
    ATGATATCGG TGTTCTTTGT GATCTTCACG TTCCCAGTTG CAGATAAAGG CTGGATCCCA
    TGTTCAGAAC TGTCCACCGT GGCCATTTTC TTCACAGCAT TAAGCTACAT GAGCCTGAGT
    CCATCTGCGG CAGCCTACGC AAGAAAGGCA CTCTTCATTG AGGTGGCTTC ATCTCTGTGT
    GCATTGACCA GCAGGTTGCC AAAGGACATG GTGACGGCCA GAATGCTGGG ACAAATTTTC
    ACAACCATCC CTCTGGGGGA ATCATTGGTG TTGGAGCTCA GTGTGCCCTG TGTGCTCTAC
    ATCTACCTGT TTTATTTGTT CTTCAGTATG GCGCGCATGA GGGGCTTCAG AGGGACGTAC
    TGTTTCCTGG TGCCGTACTT GGTCTGCTTT GTCTGGTGCG AGTTCTCTGT GGTTCTGCTT
    CGGAACTCCA CCACCATCGG CCTGATCCGT ACTTGTGTTG CCTACTTCCT CTTCCTGTTC
    GCCCTACCAG TGTTAATCAT GGGTCTGATA GCTTTGCTGC TGATTCAGCT GATTAAGTGG
    TTCCTGGAGT TAGAGTTAAC TAAGATGCTG GTGACCCTGG CAGTGTGCGC CGTCCCTGTG
    ACACTGCGAC TCTGGACTCG ATTTAGCCTC TCAATTCTGA ATGTCTTCCG CTCCATCACC
    CACCGAGGTC CAGTCAAACT GATCCTCCTG TGCATCACTA CAGTGATTTT ACTCTGCGGC
    GTCTATGTTT ATAATGCTGA AGGCCTCAAG GTTTATAACT CCACACTCAC CTGGGAGGAA
    TATGGCACAC TGTGCGGCCC GCAAGCCTGG AAAGAACGAG GAATGGCTCA AACGCAGCTC
    TCCTGCAGCC ATCTGGAAGG CCATCGTGTC ACATGGACAG GAATATTCCG TTATGTTCGT
    GTAGCAGAGA AGGAAAATGG AGCTCAGTCT GTTATAAACA TGCTACCGGT GTTCATGGGC
    GACTGGTTGC GGTGTCTCTA CGGTGAGGTT TATCCGAAGT GTGAACCACA AAACAATTCA
    TTTCCGATCG TGAATGTTAC TCAAGTCACC ACAGGAACTG TCAACACAAC AATACTGATC
    CTAAGGCAGG AAGAGGAAGA GTTATGTCGG ATAAAAGCTT ATGCTACACA CCAATGCCAC
    GTTAAACGTT TTGACAGTTA CCGTTTCGAG GTGACGGTTG GCATGCCGGT GGATGGTGTC
    ACAAAAGTGG ACAATCCAGC AGGTGACATT CTCTTAATGG CAAGTCATGA ATTCAGACAA
    GTGCTGCTGA ATCTTAACCC AGGCAGCATG GTGGAGTTCA GCACAAAACT GGAGGGAAAA
    TTGGGTAGCA AACTACCTGC GTTTGAACTG AAAGCCATAC ACTGCCTGAA CTGCAGCTCG
    AATCTGGTGC CTGAGGGACG ACAGGTCAAA ATTGAGCGCA ACTGGAGGAA GAGCATGCTT
    AAAGCCATTA AATTTGCATT TGACTTCTTC TTTGCTCCTT TCCTTTGTGC TAGGATCAAC
    GTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_695252.5
    CDS136..3321
    Translation

    Target ORF information:

    RefSeq Version XM_690160.9
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Wolfram syndrome 1a (wolframin) (wfs1a), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_690160.9

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATCTTA ATGCCAACCC ACCATCATCT CCTGAGACAT CCAAGGAGAA AAGTGCAACC 
    CATAAACGTC TCAACCCTCC CGTTCCAGCC ACCAAATCCA TTCCAGGCAC AGAGACGGCC
    GGTCCTCCTC TCTCCAAATC AGGCAGCCTA GAAAAACCAT CCCCTTCTTC AACTCTGGAT
    ACTAAATTAA AACACGGCAG TGTTAAAACC TCAAAAGTTC CAGCTGAACC AACACCTGAG
    AAACAACCCA CCCCTGCATC ATCACATATC ATCTCTAAAC CTGTTCCATC TATGCAAGCC
    CCAACATCAT CAAAAACAAC ACAGCCAATA TTATCTTCTC CCAATGAACC ATCCGCACCA
    AGCATTGCCA TTTCCGCTGA CCCACCCCCA CCTACAGCTC CTCCTGAGGG CTTAACGTCC
    ACCACCAAAA CACCTGCATC TGACTCCGGT AAACTTCCCC GCCAGCGGAG CTTTTTGGCT
    GCGGCACAAA AGGTGATGAT GCAGGAGAAA ACAAGAAAGG CTGAGGAACA GGCTAAAGCT
    GAAGAGTGTG ATGATATGGA AGACGATCTG CCTTTTGAGG AGCTCCAGAA AAAAGCTGAA
    GCAGGAGACC CTAGAGCACA GAGTCGACTC GGTAGATACT ACCTTAAACT GGCAGAAGAA
    AAAGACGCAG AGAAAAATAA CCTCACAGCT GTCGAATGGT TGATGAAGGC TGCAAAACAA
    GGCCGAAGGG ATGCAGCTAA ATTACTTCAG AAATGCTGGA TGCAAAAGAA AGGTATTACA
    GCAGAGAACG CACAGGAGGT GAGATGTCTG TCTTCAGAGA GTAAGTTTGA GCAGGCAGTG
    AGGAGAGCAG CCATGACCAT GTACTGGAAA CTCAATCCCG ACAGAAAGAA GAAAGTAGCC
    ATGTCTGAGA TGCTGGAGAA TGTCAGCCAG GTCAATACTG TACCAGGTGA AGCTTCTGTA
    TGTGGTGTAG CGCCCAGCAT TCAAACTCAG AGGAAAATCC TGGAGACAAT GGTGTCCAAT
    GAATCTAGCT CTAAATATGT GGATGTGGAA GATTTTGTGG AGATGACAAA AAAGTTCACA
    GAAGGCATTT CCCGTACTCC CACACTAAGC CCCTCTGGTG TATATGCAAA TGAAGAGGAG
    GGGATATTAC ATAAAAGCGA TGTAGCCAGA TTTGATAAGG AAAAAAACGA AATGGCTCCT
    GTTACTCATA AGCGCCAACG AAAGTCTAGC TGGGGAATGA GGAGCAGTGG GTTGATGCTG
    ACTACCAGAC GCAGCGATGC CATGAGGAGA GCCCTGGACA TAAAAGCTCA CTTTTTGGGT
    CTGCAATATC CTCTTCATGC CATTATTGAT ATGAAGGAAC ATTTAATTGA CTGGGCGTCT
    CGTGCTGGTG TCCAGTGGCT GAGCACTATT ATCCCGACAC AACATGTCAA TGCCCTTATC
    TTCTTCTTCA TCATCTCCAA TCTCACCATC GATTTGTTTG CCTTCGCCAT CCCGCTCCTC
    ATCTTCTACC TGTCCTTTAT TTCCATGGCC ATCTGCACAC TGAGAGTCTT CCAGAGCAGC
    AAAGGTTGGG AGAACTTCCG TGCCTTTACC GCTCTCCTCA CACACTTTGA GCCCAGCCTG
    GATGTAGAGC AAGCGGAGAA CAACTTTGGC TGGAACAACC TGGAGCAGTA CCTCTACTTC
    ATGATATCGG TGTTCTTTGT GATCTTCACG TTCCCAGTTG CAGATAAAGG CTGGATCCCA
    TGTTCAGAAC TGTCCACCGT GGCCATTTTC TTCACAGCAT TAAGCTACAT GAGCCTGAGT
    CCATCTGCGG CAGCCTACGC AAGAAAGGCA CTCTTCATTG AGGTGGCTTC ATCTCTGTGT
    GCATTGACCA GCAGGTTGCC AAAGGACATG GTGACGGCCA GAATGCTGGG ACAAATTTTC
    ACAACCATCC CTCTGGGGGA ATCATTGGTG TTGGAGCTCA GTGTGCCCTG TGTGCTCTAC
    ATCTACCTGT TTTATTTGTT CTTCAGTATG GCGCGCATGA GGGGCTTCAG AGGGACGTAC
    TGTTTCCTGG TGCCGTACTT GGTCTGCTTT GTCTGGTGCG AGTTCTCTGT GGTTCTGCTT
    CGGAACTCCA CCACCATCGG CCTGATCCGT ACTTGTGTTG CCTACTTCCT CTTCCTGTTC
    GCCCTACCAG TGTTAATCAT GGGTCTGATA GCTTTGCTGC TGATTCAGCT GATTAAGTGG
    TTCCTGGAGT TAGAGTTAAC TAAGATGCTG GTGACCCTGG CAGTGTGCGC CGTCCCTGTG
    ACACTGCGAC TCTGGACTCG ATTTAGCCTC TCAATTCTGA ATGTCTTCCG CTCCATCACC
    CACCGAGGTC CAGTCAAACT GATCCTCCTG TGCATCACTA CAGTGATTTT ACTCTGCGGC
    GTCTATGTTT ATAATGCTGA AGGCCTCAAG GTTTATAACT CCACACTCAC CTGGGAGGAA
    TATGGCACAC TGTGCGGCCC GCAAGCCTGG AAAGAACGAG GAATGGCTCA AACGCAGCTC
    TCCTGCAGCC ATCTGGAAGG CCATCGTGTC ACATGGACAG GAATATTCCG TTATGTTCGT
    GTAGCAGAGA AGGAAAATGG AGCTCAGTCT GTTATAAACA TGCTACCGGT GTTCATGGGC
    GACTGGTTGC GGTGTCTCTA CGGTGAGGTT TATCCGAAGT GTGAACCACA AAACAATTCA
    TTTCCGATCG TGAATGTTAC TCAAGTCACC ACAGGAACTG TCAACACAAC AATACTGATC
    CTAAGGCAGG AAGAGGAAGA GTTATGTCGG ATAAAAGCTT ATGCTACACA CCAATGCCAC
    GTTAAACGTT TTGACAGTTA CCGTTTCGAG GTGACGGTTG GCATGCCGGT GGATGGTGTC
    ACAAAAGTGG ACAATCCAGC AGGTGACATT CTCTTAATGG CAAGTCATGA ATTCAGACAA
    GTGCTGCTGA ATCTTAACCC AGGCAGCATG GTGGAGTTCA GCACAAAACT GGAGGGAAAA
    TTGGGTAGCA AACTACCTGC GTTTGAACTG AAAGCCATAC ACTGCCTGAA CTGCAGCTCG
    AATCTGGTGC CTGAGGGACG ACAGGTCAAA ATTGAGCGCA ACTGGAGGAA GAGCATGCTT
    AAAGCCATTA AATTTGCATT TGACTTCTTC TTTGCTCCTT TCCTTTGTGC TAGGATCAAC
    GTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa05550
    Clone ID Related Accession (Same CDS sequence) XM_690160.9 , XM_690160.8
    Accession Version XM_690160.8 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3186bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product wolframin
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_690160.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATCTTA ATGCCAACCC ACCATCATCT CCTGAGACAT CCAAGGAGAA AAGTGCAACC 
    CATAAACGTC TCAACCCTCC CGTTCCAGCC ACCAAATCCA TTCCAGGCAC AGAGACGGCC
    GGTCCTCCTC TCTCCAAATC AGGCAGCCTA GAAAAACCAT CCCCTTCTTC AACTCTGGAT
    ACTAAATTAA AACACGGCAG TGTTAAAACC TCAAAAGTTC CAGCTGAACC AACACCTGAG
    AAACAACCCA CCCCTGCATC ATCACATATC ATCTCTAAAC CTGTTCCATC TATGCAAGCC
    CCAACATCAT CAAAAACAAC ACAGCCAATA TTATCTTCTC CCAATGAACC ATCCGCACCA
    AGCATTGCCA TTTCCGCTGA CCCACCCCCA CCTACAGCTC CTCCTGAGGG CTTAACGTCC
    ACCACCAAAA CACCTGCATC TGACTCCGGT AAACTTCCCC GCCAGCGGAG CTTTTTGGCT
    GCGGCACAAA AGGTGATGAT GCAGGAGAAA ACAAGAAAGG CTGAGGAACA GGCTAAAGCT
    GAAGAGTGTG ATGATATGGA AGACGATCTG CCTTTTGAGG AGCTCCAGAA AAAAGCTGAA
    GCAGGAGACC CTAGAGCACA GAGTCGACTC GGTAGATACT ACCTTAAACT GGCAGAAGAA
    AAAGACGCAG AGAAAAATAA CCTCACAGCT GTCGAATGGT TGATGAAGGC TGCAAAACAA
    GGCCGAAGGG ATGCAGCTAA ATTACTTCAG AAATGCTGGA TGCAAAAGAA AGGTATTACA
    GCAGAGAACG CACAGGAGGT GAGATGTCTG TCTTCAGAGA GTAAGTTTGA GCAGGCAGTG
    AGGAGAGCAG CCATGACCAT GTACTGGAAA CTCAATCCCG ACAGAAAGAA GAAAGTAGCC
    ATGTCTGAGA TGCTGGAGAA TGTCAGCCAG GTCAATACTG TACCAGGTGA AGCTTCTGTA
    TGTGGTGTAG CGCCCAGCAT TCAAACTCAG AGGAAAATCC TGGAGACAAT GGTGTCCAAT
    GAATCTAGCT CTAAATATGT GGATGTGGAA GATTTTGTGG AGATGACAAA AAAGTTCACA
    GAAGGCATTT CCCGTACTCC CACACTAAGC CCCTCTGGTG TATATGCAAA TGAAGAGGAG
    GGGATATTAC ATAAAAGCGA TGTAGCCAGA TTTGATAAGG AAAAAAACGA AATGGCTCCT
    GTTACTCATA AGCGCCAACG AAAGTCTAGC TGGGGAATGA GGAGCAGTGG GTTGATGCTG
    ACTACCAGAC GCAGCGATGC CATGAGGAGA GCCCTGGACA TAAAAGCTCA CTTTTTGGGT
    CTGCAATATC CTCTTCATGC CATTATTGAT ATGAAGGAAC ATTTAATTGA CTGGGCGTCT
    CGTGCTGGTG TCCAGTGGCT GAGCACTATT ATCCCGACAC AACATGTCAA TGCCCTTATC
    TTCTTCTTCA TCATCTCCAA TCTCACCATC GATTTGTTTG CCTTCGCCAT CCCGCTCCTC
    ATCTTCTACC TGTCCTTTAT TTCCATGGCC ATCTGCACAC TGAGAGTCTT CCAGAGCAGC
    AAAGGTTGGG AGAACTTCCG TGCCTTTACC GCTCTCCTCA CACACTTTGA GCCCAGCCTG
    GATGTAGAGC AAGCGGAGAA CAACTTTGGC TGGAACAACC TGGAGCAGTA CCTCTACTTC
    ATGATATCGG TGTTCTTTGT GATCTTCACG TTCCCAGTTG CAGATAAAGG CTGGATCCCA
    TGTTCAGAAC TGTCCACCGT GGCCATTTTC TTCACAGCAT TAAGCTACAT GAGCCTGAGT
    CCATCTGCGG CAGCCTACGC AAGAAAGGCA CTCTTCATTG AGGTGGCTTC ATCTCTGTGT
    GCATTGACCA GCAGGTTGCC AAAGGACATG GTGACGGCCA GAATGCTGGG ACAAATTTTC
    ACAACCATCC CTCTGGGGGA ATCATTGGTG TTGGAGCTCA GTGTGCCCTG TGTGCTCTAC
    ATCTACCTGT TTTATTTGTT CTTCAGTATG GCGCGCATGA GGGGCTTCAG AGGGACGTAC
    TGTTTCCTGG TGCCGTACTT GGTCTGCTTT GTCTGGTGCG AGTTCTCTGT GGTTCTGCTT
    CGGAACTCCA CCACCATCGG CCTGATCCGT ACTTGTGTTG CCTACTTCCT CTTCCTGTTC
    GCCCTACCAG TGTTAATCAT GGGTCTGATA GCTTTGCTGC TGATTCAGCT GATTAAGTGG
    TTCCTGGAGT TAGAGTTAAC TAAGATGCTG GTGACCCTGG CAGTGTGCGC CGTCCCTGTG
    ACACTGCGAC TCTGGACTCG ATTTAGCCTC TCAATTCTGA ATGTCTTCCG CTCCATCACC
    CACCGAGGTC CAGTCAAACT GATCCTCCTG TGCATCACTA CAGTGATTTT ACTCTGCGGC
    GTCTATGTTT ATAATGCTGA AGGCCTCAAG GTTTATAACT CCACACTCAC CTGGGAGGAA
    TATGGCACAC TGTGCGGCCC GCAAGCCTGG AAAGAACGAG GAATGGCTCA AACGCAGCTC
    TCCTGCAGCC ATCTGGAAGG CCATCGTGTC ACATGGACAG GAATATTCCG TTATGTTCGT
    GTAGCAGAGA AGGAAAATGG AGCTCAGTCT GTTATAAACA TGCTACCGGT GTTCATGGGC
    GACTGGTTGC GGTGTCTCTA CGGTGAGGTT TATCCGAAGT GTGAACCACA AAACAATTCA
    TTTCCGATCG TGAATGTTAC TCAAGTCACC ACAGGAACTG TCAACACAAC AATACTGATC
    CTAAGGCAGG AAGAGGAAGA GTTATGTCGG ATAAAAGCTT ATGCTACACA CCAATGCCAC
    GTTAAACGTT TTGACAGTTA CCGTTTCGAG GTGACGGTTG GCATGCCGGT GGATGGTGTC
    ACAAAAGTGG ACAATCCAGC AGGTGACATT CTCTTAATGG CAAGTCATGA ATTCAGACAA
    GTGCTGCTGA ATCTTAACCC AGGCAGCATG GTGGAGTTCA GCACAAAACT GGAGGGAAAA
    TTGGGTAGCA AACTACCTGC GTTTGAACTG AAAGCCATAC ACTGCCTGAA CTGCAGCTCG
    AATCTGGTGC CTGAGGGACG ACAGGTCAAA ATTGAGCGCA ACTGGAGGAA GAGCATGCTT
    AAAGCCATTA AATTTGCATT TGACTTCTTC TTTGCTCCTT TCCTTTGTGC TAGGATCAAC
    GTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_695252.5
    CDS136..3321
    Translation

    Target ORF information:

    RefSeq Version XM_690160.8
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Wolfram syndrome 1a (wolframin) (wfs1a), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_690160.8

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATCTTA ATGCCAACCC ACCATCATCT CCTGAGACAT CCAAGGAGAA AAGTGCAACC 
    CATAAACGTC TCAACCCTCC CGTTCCAGCC ACCAAATCCA TTCCAGGCAC AGAGACGGCC
    GGTCCTCCTC TCTCCAAATC AGGCAGCCTA GAAAAACCAT CCCCTTCTTC AACTCTGGAT
    ACTAAATTAA AACACGGCAG TGTTAAAACC TCAAAAGTTC CAGCTGAACC AACACCTGAG
    AAACAACCCA CCCCTGCATC ATCACATATC ATCTCTAAAC CTGTTCCATC TATGCAAGCC
    CCAACATCAT CAAAAACAAC ACAGCCAATA TTATCTTCTC CCAATGAACC ATCCGCACCA
    AGCATTGCCA TTTCCGCTGA CCCACCCCCA CCTACAGCTC CTCCTGAGGG CTTAACGTCC
    ACCACCAAAA CACCTGCATC TGACTCCGGT AAACTTCCCC GCCAGCGGAG CTTTTTGGCT
    GCGGCACAAA AGGTGATGAT GCAGGAGAAA ACAAGAAAGG CTGAGGAACA GGCTAAAGCT
    GAAGAGTGTG ATGATATGGA AGACGATCTG CCTTTTGAGG AGCTCCAGAA AAAAGCTGAA
    GCAGGAGACC CTAGAGCACA GAGTCGACTC GGTAGATACT ACCTTAAACT GGCAGAAGAA
    AAAGACGCAG AGAAAAATAA CCTCACAGCT GTCGAATGGT TGATGAAGGC TGCAAAACAA
    GGCCGAAGGG ATGCAGCTAA ATTACTTCAG AAATGCTGGA TGCAAAAGAA AGGTATTACA
    GCAGAGAACG CACAGGAGGT GAGATGTCTG TCTTCAGAGA GTAAGTTTGA GCAGGCAGTG
    AGGAGAGCAG CCATGACCAT GTACTGGAAA CTCAATCCCG ACAGAAAGAA GAAAGTAGCC
    ATGTCTGAGA TGCTGGAGAA TGTCAGCCAG GTCAATACTG TACCAGGTGA AGCTTCTGTA
    TGTGGTGTAG CGCCCAGCAT TCAAACTCAG AGGAAAATCC TGGAGACAAT GGTGTCCAAT
    GAATCTAGCT CTAAATATGT GGATGTGGAA GATTTTGTGG AGATGACAAA AAAGTTCACA
    GAAGGCATTT CCCGTACTCC CACACTAAGC CCCTCTGGTG TATATGCAAA TGAAGAGGAG
    GGGATATTAC ATAAAAGCGA TGTAGCCAGA TTTGATAAGG AAAAAAACGA AATGGCTCCT
    GTTACTCATA AGCGCCAACG AAAGTCTAGC TGGGGAATGA GGAGCAGTGG GTTGATGCTG
    ACTACCAGAC GCAGCGATGC CATGAGGAGA GCCCTGGACA TAAAAGCTCA CTTTTTGGGT
    CTGCAATATC CTCTTCATGC CATTATTGAT ATGAAGGAAC ATTTAATTGA CTGGGCGTCT
    CGTGCTGGTG TCCAGTGGCT GAGCACTATT ATCCCGACAC AACATGTCAA TGCCCTTATC
    TTCTTCTTCA TCATCTCCAA TCTCACCATC GATTTGTTTG CCTTCGCCAT CCCGCTCCTC
    ATCTTCTACC TGTCCTTTAT TTCCATGGCC ATCTGCACAC TGAGAGTCTT CCAGAGCAGC
    AAAGGTTGGG AGAACTTCCG TGCCTTTACC GCTCTCCTCA CACACTTTGA GCCCAGCCTG
    GATGTAGAGC AAGCGGAGAA CAACTTTGGC TGGAACAACC TGGAGCAGTA CCTCTACTTC
    ATGATATCGG TGTTCTTTGT GATCTTCACG TTCCCAGTTG CAGATAAAGG CTGGATCCCA
    TGTTCAGAAC TGTCCACCGT GGCCATTTTC TTCACAGCAT TAAGCTACAT GAGCCTGAGT
    CCATCTGCGG CAGCCTACGC AAGAAAGGCA CTCTTCATTG AGGTGGCTTC ATCTCTGTGT
    GCATTGACCA GCAGGTTGCC AAAGGACATG GTGACGGCCA GAATGCTGGG ACAAATTTTC
    ACAACCATCC CTCTGGGGGA ATCATTGGTG TTGGAGCTCA GTGTGCCCTG TGTGCTCTAC
    ATCTACCTGT TTTATTTGTT CTTCAGTATG GCGCGCATGA GGGGCTTCAG AGGGACGTAC
    TGTTTCCTGG TGCCGTACTT GGTCTGCTTT GTCTGGTGCG AGTTCTCTGT GGTTCTGCTT
    CGGAACTCCA CCACCATCGG CCTGATCCGT ACTTGTGTTG CCTACTTCCT CTTCCTGTTC
    GCCCTACCAG TGTTAATCAT GGGTCTGATA GCTTTGCTGC TGATTCAGCT GATTAAGTGG
    TTCCTGGAGT TAGAGTTAAC TAAGATGCTG GTGACCCTGG CAGTGTGCGC CGTCCCTGTG
    ACACTGCGAC TCTGGACTCG ATTTAGCCTC TCAATTCTGA ATGTCTTCCG CTCCATCACC
    CACCGAGGTC CAGTCAAACT GATCCTCCTG TGCATCACTA CAGTGATTTT ACTCTGCGGC
    GTCTATGTTT ATAATGCTGA AGGCCTCAAG GTTTATAACT CCACACTCAC CTGGGAGGAA
    TATGGCACAC TGTGCGGCCC GCAAGCCTGG AAAGAACGAG GAATGGCTCA AACGCAGCTC
    TCCTGCAGCC ATCTGGAAGG CCATCGTGTC ACATGGACAG GAATATTCCG TTATGTTCGT
    GTAGCAGAGA AGGAAAATGG AGCTCAGTCT GTTATAAACA TGCTACCGGT GTTCATGGGC
    GACTGGTTGC GGTGTCTCTA CGGTGAGGTT TATCCGAAGT GTGAACCACA AAACAATTCA
    TTTCCGATCG TGAATGTTAC TCAAGTCACC ACAGGAACTG TCAACACAAC AATACTGATC
    CTAAGGCAGG AAGAGGAAGA GTTATGTCGG ATAAAAGCTT ATGCTACACA CCAATGCCAC
    GTTAAACGTT TTGACAGTTA CCGTTTCGAG GTGACGGTTG GCATGCCGGT GGATGGTGTC
    ACAAAAGTGG ACAATCCAGC AGGTGACATT CTCTTAATGG CAAGTCATGA ATTCAGACAA
    GTGCTGCTGA ATCTTAACCC AGGCAGCATG GTGGAGTTCA GCACAAAACT GGAGGGAAAA
    TTGGGTAGCA AACTACCTGC GTTTGAACTG AAAGCCATAC ACTGCCTGAA CTGCAGCTCG
    AATCTGGTGC CTGAGGGACG ACAGGTCAAA ATTGAGCGCA ACTGGAGGAA GAGCATGCTT
    AAAGCCATTA AATTTGCATT TGACTTCTTC TTTGCTCCTT TCCTTTGTGC TAGGATCAAC
    GTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.