SI:CH211-208H7.4 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	SI:CH211-208H7.4
Entrez Gene ID	565444
Full Name	transmembrane 131 like
Synonyms	fi34g09,si:ch211-208h7.4,wu:fi34g09
General protein information	Preferred Names transmembrane 131 like Names transmembrane protein 131-like
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009291318.2	XP_009289593.2	transmembrane protein 131-like isoform X3
XM_688724.8	XP_693816.8	transmembrane protein 131-like isoform X1
XM_009291318.3	XP_009289593.3	transmembrane protein 131-like isoform X3
XM_688724.7	XP_693816.7	transmembrane protein 131-like isoform X1
XM_005170953.4	XP_005171010.4	transmembrane protein 131-like isoform X2
XM_005170953.3	XP_005171010.3	transmembrane protein 131-like isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	KIAA0922	XP_517586.3
	LOC696761	XP_001087585.2
Xenopus tropicalis (tropical clawed frog)	kiaa0922	XP_002933525.1
Rattus norvegicus (Norway rat)	LOC679811	XP_001054550.1
Danio rerio (zebrafish)	si:ch211-208h7.4	XP_693816.5
Bos taurus (cattle)	KIAA0922	NP_001070271.2
Mus musculus (house mouse)	D930015E06Rik	NP_766269.3
Gallus gallus (chicken)	KIAA0922	XP_004940936.1
Homo sapiens (human)	KIAA0922	NP_001124479.1
Canis lupus familiaris (dog)	KIAA0922	XP_005629419.1

Gene Ontology

The following SI:CH211-208H7.4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SI:CH211-208H7.4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa44610	XM_688724.8 Latest version!	Danio rerio transmembrane 131 like (tmem131l), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa44611	XM_005170953.4 Latest version!	Danio rerio transmembrane 131 like (tmem131l), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa44612	XM_009291318.3 Latest version!	Danio rerio transmembrane 131 like (tmem131l), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa53886	XM_688724.7 Latest version!	Danio rerio si:ch211-208h7.4 (si:ch211-208h7.4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa53887	XM_005170953.3 Latest version!	Danio rerio si:ch211-208h7.4 (si:ch211-208h7.4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa53888	XM_009291318.2 Latest version!	Danio rerio si:ch211-208h7.4 (si:ch211-208h7.4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SI:CH211-208H7.4 ( XM_688724.8 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_693816.8 Danio rerio transmembrane 131 like (tmem131l), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa44610
Clone ID Related Accession (Same CDS sequence)	XM_688724.8
Accession Version	XM_688724.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transmembrane protein 131-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_688724.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCC AAAGGGACTT CACACACAGA AAAACATGGA TTAATGTTCT CCTGCGACTC 
TTACAGCTCT TATTGCTTTA CATTCAGCAC GGAGGAGCTC AGTTTCAAGC TCTGACTCAG 
ATGTCCAGCG TGGTCGAAGT TTGGCAGGCT GAGGACGCAG ACTTCTTCGT GCCATCTCAG 
GCAGAGGAGC AACACAGTGA AAATGTTCGA GAGGAGAGCT CCTCGTTTTA TGTGCAGGAG 
AGTGGGAGAC AGCTCCTGTT TCAGCCGCCC ACACTGGATT TTGGACCACA GCCGGTGGGG 
CTGCCAAGAG CTGAAACCGT ATATATCCAC AATCCCAGCC CAGAGCTCCC AGTCTCTCTG 
CTTTCTGTGT TTACATCCAG CAGACATTTT CACATGCCTT CCTTCCACAG AAGGGTAATT 
CCACCCAGAG GGAGAACATC TTTTAAAATC ATTTTCCTCC CCACAGTGGA GGGCAATGAA 
GAAATCTCTT TATTTATTAA TACATCAACC CATGGGTTGT TATCATACCA GGTTTTTGGG 
GTCGGCGTCC ATGGGGAATC AGTTAAGGAC GCCCACAGAA AAGATAGCGT TCTGATATTC 
CCCCACATAC AGAGCATTAA TCTAACACAA TCTCAGGAAG ATGCTTCTAA TATAACAATT 
TTAGGTCTGC TGCTTGACTG CAGTTTACCA AAAATGTTCT ACAATCAGCC CCAGGGTTGG 
TGTTTAGTAT CAGAGGAGGG CAGAATTGCT CTTCAGATCA GTCTTTCTGA AAGAGGGGAA 
AGGCCGACCC ACCTGGACAA ACTCAAGCCA TACGTGCTGG AGAATATTGT GGTGCTGCTG 
GTCCTCCCAC CACCAGAAGA TGCTGTCTCA GATCCCAAAA TAGGAGTGTT CATGTTGAAC 
TCAGGTGTTA AAAAGCTCTT TGTTAAGGAG ATCCAGCTTT TGTCGAGAAC AGACAGCAAT 
GTTGAACTCA CTGAAGTCTT GCTCAAGCCT TCTGCTACTA ACTTCACTCA AGTGGCTTCA 
CTCTTCTGTA GACATTCTTT GACGGCTAAA AGTAAAAGGT GTTCAAGTCA GTTCGGCCTA 
CACATTTTAG CAAACAGAAC AGTGAATTTA TATCCACTGC TGCAAAGAAC ACACAGATGG 
TCAGACTCTG AGCTGAGTCC TTTAATTCAG ATCAGACAGA AGCAGAGGGG CTCAGAGCAT 
GTGGAGCTCT GGCTAACAAA CCCTTTCCCC TTCTCCTTCA GAATCACTAA CATCTCCCTG 
TCCCAGCAGA AACTCTTTAA GAGTGTAAAT TTTACAACAT CTGTGGTAGT GAGCTCGGGG 
AGCTGGAAGC TTCTCTCTCT TCAGCTACTC GGTAAATCTT TACCCATCAA CCACAGAACC 
ACAATTATCC TGGCAACCAG TGTTGGGCTC CCACTAGAGC TTCACCTGCA AACATATTCT 
TCTTCTTCAA AGCAGGGAGT TGTTATTGTT GAGTCTTCTG AGGAGTGTGA ACGGCTATGT 
CCACTCCGTA TGTCCAGGGC AGGACGTGTG GAGTGGCAGC AATCGCTTCT CCCAGAATCA 
TCCTCTTCAC TATGGAGAGT AGACAGCAGT CTGGCCTCTG CCCTGTGCAG TCGTTGGCAC 
TGTAGCAAAG AGCTCTCCTG CAGGTGGCCC AGACTTCCTG AAGATCACTC CACACCCCTA 
GACTTTGGTG CCACACCTGT CAATGATAGT AAGGTAAAAA ATTTCACTCT GAAAAACCCG 
ACGGGTTCAG TGATTTCTGT GGAGATACGA ACCCTCTCCT CATACCCAGT TCCACTTGAA 
GCACTCGACA TGCTTACCAA ATGGTTTAAT ATTAGCCCCC TCTCTGTGAA TATCACTACA 
ACAGAGTTTC TGCTGTTGGC TGCTAACCAT AAGGAAAACA AGAGAGGTAG TGTGCTGAGA 
ATCCTCCTTC AGCCGTGGGA GTCGAGGACT GTGTCTGTGG CGTATCAACC CACAGAACAC 
AAGCCTGTTA CCTCACTGCT CTTGATCAGG AACAACCTGA CCGTGTTTGA CATGGTTCTG 
GTTAAAGGAT TAGGAGCAAA GGAGATGTTG AGGGTGGGAG GGAAGCTTCC TGGACCTGCA 
GCCTCTCTGC GCTTCAATGT GCCTCAGTCC ACCCTCATGG AGTGCAGAGA TGGCTTACGA 
TCAAGTAAGC CCTTATTTGC CATCCAGAAA AGCTTTAAAG TGGAGAATGC AGGCGAGTTG 
CCGCTGACTG TAGTGTCCAT GAACATTAAT GGATATAAAT GTCAGGGTTA TGGTTTTGAA 
GTGCAGCACT GTGGATCCTT CAGAGTGGAC TACAACTCCT CCTCAGAAAT CACTATTGCA 
TTTACTCCAG ACTTCACTTC CTCATGGGTT ATTAGAGACC TGACGCTGGT GACAGAGCGA 
GGCTCCTCTT TCCATTTCAC CCTTAATGTG ACTCTTCCTC ACCACATGCT GCCGCTGTGT 
GCTCAGGTGG TCCCGGGACC CAGCTGGGAG GAGTCTTTCT GGATCATCAC ACTGGCCTTT 
ACCTGCTTCT CTCTGGCTGT TGTGTGTCTG ATGGCTTTTC ATCAAGCTCA ACACATTCTG 
AGTGAGTTCT CCATGCCAAA TCCACGTAGC AATCACAACT CGGTACCTCG AGAAAATAGC 
AGTGTTAACC ACAGCTCTTC TAATGGTGTC AGTCGAGGAA AAGGCAGTTG TAAGAACTAC 
ACTGACACAA GCCACCCGTC TGATAAAGGC AAAGGCAGAG GATCTCCAGC TGTAGCCAAT 
GGATCAGTTC GGTTGCAGCC TTCATCCAAG AAGAGCTCTG GAGGTTCTTC TCAGCCTCCA 
AAGAAGCAAA CTAAGGTTTC ATTACTATAC AGCAGATACA AAAACAGCCC TGCGACTCTG 
GCTGCTAGTG TTTCTGCTAC CACTGACTTC TGTGGTTCGC CCACCAATGA AGATAAAGAT 
CACACCTTTG AATTAGACCC AGAAATTTGT AATAACAACA ACAACAATAA TGATATATTA 
GACGAAGCCC TTCTCCCTGT CGAGAAGAAA GAGCACTTTA GAGAATCCAA GGAAGAGAAG 
GTCTTGCCAG CTGTTATGTT TCCCATGGAA ACACTTCTTG GATTACCTGA AAACATCACA 
GCAAACAGAG TCCCTCAGCC TGCAGGCGAA GTGATGGAAA ATGAAGAATG TAAAAGAAAA 
ACCAATGCAG ATAAAAGGGA TCATCTTGAT GAACAGAAAA CAGAAAGAGG CAGCAGTCAA 
AAGAAAAGAG CAGAGAAAGA TGCAGAACTT GGGGTTTCTG CTCCTATCAA CAAAACGAAG 
AAAAACGCAG GCAGGAGTCG CAAGAAGGCA CCAGAGCTCA TCCCTGGGCT TCCTGAAAAC 
AGTGTTGTTA TGGTGACAGA GAGTGAGAGA GAACCATCAC GTGCTGCTGC TCGAACACGA 
AACGCCAAAC CCGAACTTCC TAAAACAGGA GCAAACACAG ATAGTCCTCT CAAACACAGC 
GGCACGTGTT TGGGCAGACC TAGGCGTAAG TGTCCAGAGC GGCGTCTGCA GTGGGAATCT 
GGCTCTGACT CCAGCAGTTC ATCAGGCAGT GTGCGAGCCA GCCGAGGGTC CTGGGGCAGC 
TGGAGCAGTG CCAGCAGCAT AGAGGGGGAA AGAGATCCAA ACAGACACCC CTGCAGGAGA 
GACAACATTC AATACAATCC ATATTTAAAA GACCAGGAAT GTTACCTGAA CATGAACTAT 
ACCTGCAAAA ATCCAAGCAT GAACAATCTT TATGACAGAG AACCATACCA AAGCTCAGAA 
ATGGGTCCTG TCCCAGCACT TCCTCACACC TTCGCTGATG TAGCTGCAGG TGTGGACAAA 
AACATCGACG TAATTGGCTC CAGCATGATG GAAGAGACGT GGTCAGTCAA CTCCGTTCCT 
CTGACCAATG AATTCAGGTA CAACATGAGT GAATCTCTCC CGTTTGTCCC TCAGAATCCC 
TCATCCAGAT ATGATGGCTT CCCTTGGAAC AACATGAGCA GTCAGTGTAG CAGTCAGTAT 
CCCTACAGTG ACCGCAGCAA CTACATGCCA ACAGGTAACG GCAATTACCA AAATGCATTC 
TCATGCCCTG AAAGCCAAAC CATAAGCCAC AGCCATCAGC CGGTGTGGGG TGAGAATGGA 
GCCCATGATA TCAACTCTAC ATGGGACACT GGCAGCTGTG TGGGCAGCAA GCCATACTTT 
TCAGGAACCA GAAGTCTGTC CCCCATGTCC AGTCTATTTG GTTCAATCTG GACACCCCAA 
AGCGAGCCTT ACCAGAGTCA CTTCCACCCA GAGCGTTCAG CACCAGTCTC GCCTCACACC 
CCCCATACAC CCATCTCACC GGTCGCCCCC TTCAGCCGTG AACCCGGCGG CGCCTGTCCC 
GGGAAGCACT TCTCCAGCTT CAACCCCTTC GGACCACACA TGAATTTGGA CATATGGAAC 
TCCTCTTCAA ACCGGAGCTC CAACTCTCAG CTCTCCAATG ATTCAGGCTA CTGCGGTGAC 
ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_693816.8
CDS	126..4631
Translation	MASQRDFTHRKTWINVLLRLLQLLLLYIQHGGAQFQALTQMSSVVEVWQAEDADFFVPSQAEEQHSENVREESSSFYVQESGRQLLFQPPTLDFGPQPVGLPRAETVYIHNPSPELPVSLLSVFTSSRHFHMPSFHRRVIPPRGRTSFKIIFLPTVEGNEEISLFINTSTHGLLSYQVFGVGVHGESVKDAHRKDSVLIFPHIQSINLTQSQEDASNITILGLLLDCSLPKMFYNQPQGWCLVSEEGRIALQISLSERGERPTHLDKLKPYVLENIVVLLVLPPPEDAVSDPKIGVFMLNSGVKKLFVKEIQLLSRTDSNVELTEVLLKPSATNFTQVASLFCRHSLTAKSKRCSSQFGLHILANRTVNLYPLLQRTHRWSDSELSPLIQIRQKQRGSEHVELWLTNPFPFSFRITNISLSQQKLFKSVNFTTSVVVSSGSWKLLSLQLLGKSLPINHRTTIILATSVGLPLELHLQTYSSSSKQGVVIVESSEECERLCPLRMSRAGRVEWQQSLLPESSSSLWRVDSSLASALCSRWHCSKELSCRWPRLPEDHSTPLDFGATPVNDSKVKNFTLKNPTGSVISVEIRTLSSYPVPLEALDMLTKWFNISPLSVNITTTEFLLLAANHKENKRGSVLRILLQPWESRTVSVAYQPTEHKPVTSLLLIRNNLTVFDMVLVKGLGAKEMLRVGGKLPGPAASLRFNVPQSTLMECRDGLRSSKPLFAIQKSFKVENAGELPLTVVSMNINGYKCQGYGFEVQHCGSFRVDYNSSSEITIAFTPDFTSSWVIRDLTLVTERGSSFHFTLNVTLPHHMLPLCAQVVPGPSWEESFWIITLAFTCFSLAVVCLMAFHQAQHILSEFSMPNPRSNHNSVPRENSSVNHSSSNGVSRGKGSCKNYTDTSHPSDKGKGRGSPAVANGSVRLQPSSKKSSGGSSQPPKKQTKVSLLYSRYKNSPATLAASVSATTDFCGSPTNEDKDHTFELDPEICNNNNNNNDILDEALLPVEKKEHFRESKEEKVLPAVMFPMETLLGLPENITANRVPQPAGEVMENEECKRKTNADKRDHLDEQKTERGSSQKKRAEKDAELGVSAPINKTKKNAGRSRKKAPELIPGLPENSVVMVTESEREPSRAAARTRNAKPELPKTGANTDSPLKHSGTCLGRPRRKCPERRLQWESGSDSSSSSGSVRASRGSWGSWSSASSIEGERDPNRHPCRRDNIQYNPYLKDQECYLNMNYTCKNPSMNNLYDREPYQSSEMGPVPALPHTFADVAAGVDKNIDVIGSSMMEETWSVNSVPLTNEFRYNMSESLPFVPQNPSSRYDGFPWNNMSSQCSSQYPYSDRSNYMPTGNGNYQNAFSCPESQTISHSHQPVWGENGAHDINSTWDTGSCVGSKPYFSGTRSLSPMSSLFGSIWTPQSEPYQSHFHPERSAPVSPHTPHTPISPVAPFSREPGGACPGKHFSSFNPFGPHMNLDIWNSSSNRSSNSQLSNDSGYCGDM

Target ORF information:

RefSeq Version	XM_688724.8
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transmembrane 131 like (tmem131l), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_688724.8

ORF Insert Sequence:

ATGGCGAGCC AAAGGGACTT CACACACAGA AAAACATGGA TTAATGTTCT CCTGCGACTC 
TTACAGCTCT TATTGCTTTA CATTCAGCAC GGAGGAGCTC AGTTTCAAGC TCTGACTCAG 
ATGTCCAGCG TGGTCGAAGT TTGGCAGGCT GAGGACGCAG ACTTCTTCGT GCCATCTCAG 
GCAGAGGAGC AACACAGTGA AAATGTTCGA GAGGAGAGCT CCTCGTTTTA TGTGCAGGAG 
AGTGGGAGAC AGCTCCTGTT TCAGCCGCCC ACACTGGATT TTGGACCACA GCCGGTGGGG 
CTGCCAAGAG CTGAAACCGT ATATATCCAC AATCCCAGCC CAGAGCTCCC AGTCTCTCTG 
CTTTCTGTGT TTACATCCAG CAGACATTTT CACATGCCTT CCTTCCACAG AAGGGTAATT 
CCACCCAGAG GGAGAACATC TTTTAAAATC ATTTTCCTCC CCACAGTGGA GGGCAATGAA 
GAAATCTCTT TATTTATTAA TACATCAACC CATGGGTTGT TATCATACCA GGTTTTTGGG 
GTCGGCGTCC ATGGGGAATC AGTTAAGGAC GCCCACAGAA AAGATAGCGT TCTGATATTC 
CCCCACATAC AGAGCATTAA TCTAACACAA TCTCAGGAAG ATGCTTCTAA TATAACAATT 
TTAGGTCTGC TGCTTGACTG CAGTTTACCA AAAATGTTCT ACAATCAGCC CCAGGGTTGG 
TGTTTAGTAT CAGAGGAGGG CAGAATTGCT CTTCAGATCA GTCTTTCTGA AAGAGGGGAA 
AGGCCGACCC ACCTGGACAA ACTCAAGCCA TACGTGCTGG AGAATATTGT GGTGCTGCTG 
GTCCTCCCAC CACCAGAAGA TGCTGTCTCA GATCCCAAAA TAGGAGTGTT CATGTTGAAC 
TCAGGTGTTA AAAAGCTCTT TGTTAAGGAG ATCCAGCTTT TGTCGAGAAC AGACAGCAAT 
GTTGAACTCA CTGAAGTCTT GCTCAAGCCT TCTGCTACTA ACTTCACTCA AGTGGCTTCA 
CTCTTCTGTA GACATTCTTT GACGGCTAAA AGTAAAAGGT GTTCAAGTCA GTTCGGCCTA 
CACATTTTAG CAAACAGAAC AGTGAATTTA TATCCACTGC TGCAAAGAAC ACACAGATGG 
TCAGACTCTG AGCTGAGTCC TTTAATTCAG ATCAGACAGA AGCAGAGGGG CTCAGAGCAT 
GTGGAGCTCT GGCTAACAAA CCCTTTCCCC TTCTCCTTCA GAATCACTAA CATCTCCCTG 
TCCCAGCAGA AACTCTTTAA GAGTGTAAAT TTTACAACAT CTGTGGTAGT GAGCTCGGGG 
AGCTGGAAGC TTCTCTCTCT TCAGCTACTC GGTAAATCTT TACCCATCAA CCACAGAACC 
ACAATTATCC TGGCAACCAG TGTTGGGCTC CCACTAGAGC TTCACCTGCA AACATATTCT 
TCTTCTTCAA AGCAGGGAGT TGTTATTGTT GAGTCTTCTG AGGAGTGTGA ACGGCTATGT 
CCACTCCGTA TGTCCAGGGC AGGACGTGTG GAGTGGCAGC AATCGCTTCT CCCAGAATCA 
TCCTCTTCAC TATGGAGAGT AGACAGCAGT CTGGCCTCTG CCCTGTGCAG TCGTTGGCAC 
TGTAGCAAAG AGCTCTCCTG CAGGTGGCCC AGACTTCCTG AAGATCACTC CACACCCCTA 
GACTTTGGTG CCACACCTGT CAATGATAGT AAGGTAAAAA ATTTCACTCT GAAAAACCCG 
ACGGGTTCAG TGATTTCTGT GGAGATACGA ACCCTCTCCT CATACCCAGT TCCACTTGAA 
GCACTCGACA TGCTTACCAA ATGGTTTAAT ATTAGCCCCC TCTCTGTGAA TATCACTACA 
ACAGAGTTTC TGCTGTTGGC TGCTAACCAT AAGGAAAACA AGAGAGGTAG TGTGCTGAGA 
ATCCTCCTTC AGCCGTGGGA GTCGAGGACT GTGTCTGTGG CGTATCAACC CACAGAACAC 
AAGCCTGTTA CCTCACTGCT CTTGATCAGG AACAACCTGA CCGTGTTTGA CATGGTTCTG 
GTTAAAGGAT TAGGAGCAAA GGAGATGTTG AGGGTGGGAG GGAAGCTTCC TGGACCTGCA 
GCCTCTCTGC GCTTCAATGT GCCTCAGTCC ACCCTCATGG AGTGCAGAGA TGGCTTACGA 
TCAAGTAAGC CCTTATTTGC CATCCAGAAA AGCTTTAAAG TGGAGAATGC AGGCGAGTTG 
CCGCTGACTG TAGTGTCCAT GAACATTAAT GGATATAAAT GTCAGGGTTA TGGTTTTGAA 
GTGCAGCACT GTGGATCCTT CAGAGTGGAC TACAACTCCT CCTCAGAAAT CACTATTGCA 
TTTACTCCAG ACTTCACTTC CTCATGGGTT ATTAGAGACC TGACGCTGGT GACAGAGCGA 
GGCTCCTCTT TCCATTTCAC CCTTAATGTG ACTCTTCCTC ACCACATGCT GCCGCTGTGT 
GCTCAGGTGG TCCCGGGACC CAGCTGGGAG GAGTCTTTCT GGATCATCAC ACTGGCCTTT 
ACCTGCTTCT CTCTGGCTGT TGTGTGTCTG ATGGCTTTTC ATCAAGCTCA ACACATTCTG 
AGTGAGTTCT CCATGCCAAA TCCACGTAGC AATCACAACT CGGTACCTCG AGAAAATAGC 
AGTGTTAACC ACAGCTCTTC TAATGGTGTC AGTCGAGGAA AAGGCAGTTG TAAGAACTAC 
ACTGACACAA GCCACCCGTC TGATAAAGGC AAAGGCAGAG GATCTCCAGC TGTAGCCAAT 
GGATCAGTTC GGTTGCAGCC TTCATCCAAG AAGAGCTCTG GAGGTTCTTC TCAGCCTCCA 
AAGAAGCAAA CTAAGGTTTC ATTACTATAC AGCAGATACA AAAACAGCCC TGCGACTCTG 
GCTGCTAGTG TTTCTGCTAC CACTGACTTC TGTGGTTCGC CCACCAATGA AGATAAAGAT 
CACACCTTTG AATTAGACCC AGAAATTTGT AATAACAACA ACAACAATAA TGATATATTA 
GACGAAGCCC TTCTCCCTGT CGAGAAGAAA GAGCACTTTA GAGAATCCAA GGAAGAGAAG 
GTCTTGCCAG CTGTTATGTT TCCCATGGAA ACACTTCTTG GATTACCTGA AAACATCACA 
GCAAACAGAG TCCCTCAGCC TGCAGGCGAA GTGATGGAAA ATGAAGAATG TAAAAGAAAA 
ACCAATGCAG ATAAAAGGGA TCATCTTGAT GAACAGAAAA CAGAAAGAGG CAGCAGTCAA 
AAGAAAAGAG CAGAGAAAGA TGCAGAACTT GGGGTTTCTG CTCCTATCAA CAAAACGAAG 
AAAAACGCAG GCAGGAGTCG CAAGAAGGCA CCAGAGCTCA TCCCTGGGCT TCCTGAAAAC 
AGTGTTGTTA TGGTGACAGA GAGTGAGAGA GAACCATCAC GTGCTGCTGC TCGAACACGA 
AACGCCAAAC CCGAACTTCC TAAAACAGGA GCAAACACAG ATAGTCCTCT CAAACACAGC 
GGCACGTGTT TGGGCAGACC TAGGCGTAAG TGTCCAGAGC GGCGTCTGCA GTGGGAATCT 
GGCTCTGACT CCAGCAGTTC ATCAGGCAGT GTGCGAGCCA GCCGAGGGTC CTGGGGCAGC 
TGGAGCAGTG CCAGCAGCAT AGAGGGGGAA AGAGATCCAA ACAGACACCC CTGCAGGAGA 
GACAACATTC AATACAATCC ATATTTAAAA GACCAGGAAT GTTACCTGAA CATGAACTAT 
ACCTGCAAAA ATCCAAGCAT GAACAATCTT TATGACAGAG AACCATACCA AAGCTCAGAA 
ATGGGTCCTG TCCCAGCACT TCCTCACACC TTCGCTGATG TAGCTGCAGG TGTGGACAAA 
AACATCGACG TAATTGGCTC CAGCATGATG GAAGAGACGT GGTCAGTCAA CTCCGTTCCT 
CTGACCAATG AATTCAGGTA CAACATGAGT GAATCTCTCC CGTTTGTCCC TCAGAATCCC 
TCATCCAGAT ATGATGGCTT CCCTTGGAAC AACATGAGCA GTCAGTGTAG CAGTCAGTAT 
CCCTACAGTG ACCGCAGCAA CTACATGCCA ACAGGTAACG GCAATTACCA AAATGCATTC 
TCATGCCCTG AAAGCCAAAC CATAAGCCAC AGCCATCAGC CGGTGTGGGG TGAGAATGGA 
GCCCATGATA TCAACTCTAC ATGGGACACT GGCAGCTGTG TGGGCAGCAA GCCATACTTT 
TCAGGAACCA GAAGTCTGTC CCCCATGTCC AGTCTATTTG GTTCAATCTG GACACCCCAA 
AGCGAGCCTT ACCAGAGTCA CTTCCACCCA GAGCGTTCAG CACCAGTCTC GCCTCACACC 
CCCCATACAC CCATCTCACC GGTCGCCCCC TTCAGCCGTG AACCCGGCGG CGCCTGTCCC 
GGGAAGCACT TCTCCAGCTT CAACCCCTTC GGACCACACA TGAATTTGGA CATATGGAAC 
TCCTCTTCAA ACCGGAGCTC CAACTCTCAG CTCTCCAATG ATTCAGGCTA CTGCGGTGAC 
ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-208H7.4 ( XM_005170953.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005171010.4 Danio rerio transmembrane 131 like (tmem131l), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa44611
Clone ID Related Accession (Same CDS sequence)	XM_005170953.4
Accession Version	XM_005170953.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transmembrane protein 131-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005170953.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCC AAAGGGACTT CACACACAGA AAAACATGGA TTAATGTTCT CCTGCGACTC 
TTACAGCTCT TATTGCTTTA CATTCAGCAC GGAGGAGCTC AGTTTCAAGC TCTGACTCAG 
ATGTCCAGCG TGGTCGAAGT TTGGCAGGCT GAGGACGCAG ACTTCTTCGT GCCATCTCAG 
GCAGAGGAGC AACACAGTGA AAATGTTCGA GAGGAGAGCT CCTCGTTTTA TGTGCAGGAG 
AGTGGGAGAC AGCTCCTGTT TCAGCCGCCC ACACTGGATT TTGGACCACA GCCGGTGGGG 
CTGCCAAGAG CTGAAACCGT ATATATCCAC AATCCCAGCC CAGAGCTCCC AGTCTCTCTG 
CTTTCTGTGT TTACATCCAG CAGACATTTT CACATGCCTT CCTTCCACAG AAGGGTAATT 
CCACCCAGAG GGAGAACATC TTTTAAAATC ATTTTCCTCC CCACAGTGGA GGGCAATGAA 
GAAATCTCTT TATTTATTAA TACATCAACC CATGGGTTGT TATCATACCA GGTTTTTGGG 
GTCGGCGTCC ATGGGGAATC AGTTAAGGAC GCCCACAGAA AAGATAGCGT TCTGATATTC 
CCCCACATAC AGAGCATTAA TCTAACACAA TCTCAGGAAG ATGCTTCTAA TATAACAATT 
TTAGGTCTGC TGCTTGACTG CAGTTTACCA AAAATGTTCT ACAATCAGCC CCAGGGTTGG 
TGTTTAGTAT CAGAGGAGGG CAGAATTGCT CTTCAGATCA GTCTTTCTGA AAGAGGGGAA 
AGGCCGACCC ACCTGGACAA ACTCAAGCCA TACGTGCTGG AGAATATTGT GGTGCTGCTG 
GTCCTCCCAC CACCAGAAGA TGCTGTCTCA GATCCCAAAA TAGGAGTGTT CATGTTGAAC 
TCAGGTGTTA AAAAGCTCTT TGTTAAGGAG ATCCAGCTTT TGTCGAGAAC AGACAGCAAT 
GTTGAACTCA CTGAAGTCTT GCTCAAGCCT TCTGCTACTA ACTTCACTCA AGTGGCTTCA 
CTCTTCTGTA GACATTCTTT GACGGCTAAA AGTAAAAGGT GTTCAAGTCA GTTCGGCCTA 
CACATTTTAG CAAACAGAAC AGTGAATTTA TATCCACTGC TGCAAAGAAC ACACAGATGG 
TCAGACTCTG AGCTGAGTCC TTTAATTCAG ATCAGACAGA AGCAGAGGGG CTCAGAGCAT 
GTGGAGCTCT GGCTAACAAA CCCTTTCCCC TTCTCCTTCA GAATCACTAA CATCTCCCTG 
TCCCAGCAGA AACTCTTTAA GAGTGTAAAT TTTACAACAT CTGTGGTAGT GAGCTCGGGG 
AGCTGGAAGC TTCTCTCTCT TCAGCTACTC GGTAAATCTT TACCCATCAA CCACAGAACC 
ACAATTATCC TGGCAACCAG TGTTGGGCTC CCACTAGAGC TTCACCTGCA AACATATTCT 
TCTTCTTCAA AGGGAGTTGT TATTGTTGAG TCTTCTGAGG AGTGTGAACG GCTATGTCCA 
CTCCGTATGT CCAGGGCAGG ACGTGTGGAG TGGCAGCAAT CGCTTCTCCC AGAATCATCC 
TCTTCACTAT GGAGAGTAGA CAGCAGTCTG GCCTCTGCCC TGTGCAGTCG TTGGCACTGT 
AGCAAAGAGC TCTCCTGCAG GTGGCCCAGA CTTCCTGAAG ATCACTCCAC ACCCCTAGAC 
TTTGGTGCCA CACCTGTCAA TGATAGTAAG GTAAAAAATT TCACTCTGAA AAACCCGACG 
GGTTCAGTGA TTTCTGTGGA GATACGAACC CTCTCCTCAT ACCCAGTTCC ACTTGAAGCA 
CTCGACATGC TTACCAAATG GTTTAATATT AGCCCCCTCT CTGTGAATAT CACTACAACA 
GAGTTTCTGC TGTTGGCTGC TAACCATAAG GAAAACAAGA GAGGTAGTGT GCTGAGAATC 
CTCCTTCAGC CGTGGGAGTC GAGGACTGTG TCTGTGGCGT ATCAACCCAC AGAACACAAG 
CCTGTTACCT CACTGCTCTT GATCAGGAAC AACCTGACCG TGTTTGACAT GGTTCTGGTT 
AAAGGATTAG GAGCAAAGGA GATGTTGAGG GTGGGAGGGA AGCTTCCTGG ACCTGCAGCC 
TCTCTGCGCT TCAATGTGCC TCAGTCCACC CTCATGGAGT GCAGAGATGG CTTACGATCA 
AGTAAGCCCT TATTTGCCAT CCAGAAAAGC TTTAAAGTGG AGAATGCAGG CGAGTTGCCG 
CTGACTGTAG TGTCCATGAA CATTAATGGA TATAAATGTC AGGGTTATGG TTTTGAAGTG 
CAGCACTGTG GATCCTTCAG AGTGGACTAC AACTCCTCCT CAGAAATCAC TATTGCATTT 
ACTCCAGACT TCACTTCCTC ATGGGTTATT AGAGACCTGA CGCTGGTGAC AGAGCGAGGC 
TCCTCTTTCC ATTTCACCCT TAATGTGACT CTTCCTCACC ACATGCTGCC GCTGTGTGCT 
CAGGTGGTCC CGGGACCCAG CTGGGAGGAG TCTTTCTGGA TCATCACACT GGCCTTTACC 
TGCTTCTCTC TGGCTGTTGT GTGTCTGATG GCTTTTCATC AAGCTCAACA CATTCTGAGT 
GAGTTCTCCA TGCCAAATCC ACGTAGCAAT CACAACTCGG TACCTCGAGA AAATAGCAGT 
GTTAACCACA GCTCTTCTAA TGGTGTCAGT CGAGGAAAAG GCAGTTGTAA GAACTACACT 
GACACAAGCC ACCCGTCTGA TAAAGGCAAA GGCAGAGGAT CTCCAGCTGT AGCCAATGGA 
TCAGTTCGGT TGCAGCCTTC ATCCAAGAAG AGCTCTGGAG GTTCTTCTCA GCCTCCAAAG 
AAGCAAACTA AGGTTTCATT ACTATACAGC AGATACAAAA ACAGCCCTGC GACTCTGGCT 
GCTAGTGTTT CTGCTACCAC TGACTTCTGT GGTTCGCCCA CCAATGAAGA TAAAGATCAC 
ACCTTTGAAT TAGACCCAGA AATTTGTAAT AACAACAACA ACAATAATGA TATATTAGAC 
GAAGCCCTTC TCCCTGTCGA GAAGAAAGAG CACTTTAGAG AATCCAAGGA AGAGAAGGTC 
TTGCCAGCTG TTATGTTTCC CATGGAAACA CTTCTTGGAT TACCTGAAAA CATCACAGCA 
AACAGAGTCC CTCAGCCTGC AGGCGAAGTG ATGGAAAATG AAGAATGTAA AAGAAAAACC 
AATGCAGATA AAAGGGATCA TCTTGATGAA CAGAAAACAG AAAGAGGCAG CAGTCAAAAG 
AAAAGAGCAG AGAAAGATGC AGAACTTGGG GTTTCTGCTC CTATCAACAA AACGAAGAAA 
AACGCAGGCA GGAGTCGCAA GAAGGCACCA GAGCTCATCC CTGGGCTTCC TGAAAACAGT 
GTTGTTATGG TGACAGAGAG TGAGAGAGAA CCATCACGTG CTGCTGCTCG AACACGAAAC 
GCCAAACCCG AACTTCCTAA AACAGGAGCA AACACAGATA GTCCTCTCAA ACACAGCGGC 
ACGTGTTTGG GCAGACCTAG GCGTAAGTGT CCAGAGCGGC GTCTGCAGTG GGAATCTGGC 
TCTGACTCCA GCAGTTCATC AGGCAGTGTG CGAGCCAGCC GAGGGTCCTG GGGCAGCTGG 
AGCAGTGCCA GCAGCATAGA GGGGGAAAGA GATCCAAACA GACACCCCTG CAGGAGAGAC 
AACATTCAAT ACAATCCATA TTTAAAAGAC CAGGAATGTT ACCTGAACAT GAACTATACC 
TGCAAAAATC CAAGCATGAA CAATCTTTAT GACAGAGAAC CATACCAAAG CTCAGAAATG 
GGTCCTGTCC CAGCACTTCC TCACACCTTC GCTGATGTAG CTGCAGGTGT GGACAAAAAC 
ATCGACGTAA TTGGCTCCAG CATGATGGAA GAGACGTGGT CAGTCAACTC CGTTCCTCTG 
ACCAATGAAT TCAGGTACAA CATGAGTGAA TCTCTCCCGT TTGTCCCTCA GAATCCCTCA 
TCCAGATATG ATGGCTTCCC TTGGAACAAC ATGAGCAGTC AGTGTAGCAG TCAGTATCCC 
TACAGTGACC GCAGCAACTA CATGCCAACA GGTAACGGCA ATTACCAAAA TGCATTCTCA 
TGCCCTGAAA GCCAAACCAT AAGCCACAGC CATCAGCCGG TGTGGGGTGA GAATGGAGCC 
CATGATATCA ACTCTACATG GGACACTGGC AGCTGTGTGG GCAGCAAGCC ATACTTTTCA 
GGAACCAGAA GTCTGTCCCC CATGTCCAGT CTATTTGGTT CAATCTGGAC ACCCCAAAGC 
GAGCCTTACC AGAGTCACTT CCACCCAGAG CGTTCAGCAC CAGTCTCGCC TCACACCCCC 
CATACACCCA TCTCACCGGT CGCCCCCTTC AGCCGTGAAC CCGGCGGCGC CTGTCCCGGG 
AAGCACTTCT CCAGCTTCAA CCCCTTCGGA CCACACATGA ATTTGGACAT ATGGAACTCC 
TCTTCAAACC GGAGCTCCAA CTCTCAGCTC TCCAATGATT CAGGCTACTG CGGTGACATG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171010.4
CDS	126..4628
Translation	MASQRDFTHRKTWINVLLRLLQLLLLYIQHGGAQFQALTQMSSVVEVWQAEDADFFVPSQAEEQHSENVREESSSFYVQESGRQLLFQPPTLDFGPQPVGLPRAETVYIHNPSPELPVSLLSVFTSSRHFHMPSFHRRVIPPRGRTSFKIIFLPTVEGNEEISLFINTSTHGLLSYQVFGVGVHGESVKDAHRKDSVLIFPHIQSINLTQSQEDASNITILGLLLDCSLPKMFYNQPQGWCLVSEEGRIALQISLSERGERPTHLDKLKPYVLENIVVLLVLPPPEDAVSDPKIGVFMLNSGVKKLFVKEIQLLSRTDSNVELTEVLLKPSATNFTQVASLFCRHSLTAKSKRCSSQFGLHILANRTVNLYPLLQRTHRWSDSELSPLIQIRQKQRGSEHVELWLTNPFPFSFRITNISLSQQKLFKSVNFTTSVVVSSGSWKLLSLQLLGKSLPINHRTTIILATSVGLPLELHLQTYSSSSKGVVIVESSEECERLCPLRMSRAGRVEWQQSLLPESSSSLWRVDSSLASALCSRWHCSKELSCRWPRLPEDHSTPLDFGATPVNDSKVKNFTLKNPTGSVISVEIRTLSSYPVPLEALDMLTKWFNISPLSVNITTTEFLLLAANHKENKRGSVLRILLQPWESRTVSVAYQPTEHKPVTSLLLIRNNLTVFDMVLVKGLGAKEMLRVGGKLPGPAASLRFNVPQSTLMECRDGLRSSKPLFAIQKSFKVENAGELPLTVVSMNINGYKCQGYGFEVQHCGSFRVDYNSSSEITIAFTPDFTSSWVIRDLTLVTERGSSFHFTLNVTLPHHMLPLCAQVVPGPSWEESFWIITLAFTCFSLAVVCLMAFHQAQHILSEFSMPNPRSNHNSVPRENSSVNHSSSNGVSRGKGSCKNYTDTSHPSDKGKGRGSPAVANGSVRLQPSSKKSSGGSSQPPKKQTKVSLLYSRYKNSPATLAASVSATTDFCGSPTNEDKDHTFELDPEICNNNNNNNDILDEALLPVEKKEHFRESKEEKVLPAVMFPMETLLGLPENITANRVPQPAGEVMENEECKRKTNADKRDHLDEQKTERGSSQKKRAEKDAELGVSAPINKTKKNAGRSRKKAPELIPGLPENSVVMVTESEREPSRAAARTRNAKPELPKTGANTDSPLKHSGTCLGRPRRKCPERRLQWESGSDSSSSSGSVRASRGSWGSWSSASSIEGERDPNRHPCRRDNIQYNPYLKDQECYLNMNYTCKNPSMNNLYDREPYQSSEMGPVPALPHTFADVAAGVDKNIDVIGSSMMEETWSVNSVPLTNEFRYNMSESLPFVPQNPSSRYDGFPWNNMSSQCSSQYPYSDRSNYMPTGNGNYQNAFSCPESQTISHSHQPVWGENGAHDINSTWDTGSCVGSKPYFSGTRSLSPMSSLFGSIWTPQSEPYQSHFHPERSAPVSPHTPHTPISPVAPFSREPGGACPGKHFSSFNPFGPHMNLDIWNSSSNRSSNSQLSNDSGYCGDM

Target ORF information:

RefSeq Version	XM_005170953.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transmembrane 131 like (tmem131l), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170953.4

ORF Insert Sequence:

ATGGCGAGCC AAAGGGACTT CACACACAGA AAAACATGGA TTAATGTTCT CCTGCGACTC 
TTACAGCTCT TATTGCTTTA CATTCAGCAC GGAGGAGCTC AGTTTCAAGC TCTGACTCAG 
ATGTCCAGCG TGGTCGAAGT TTGGCAGGCT GAGGACGCAG ACTTCTTCGT GCCATCTCAG 
GCAGAGGAGC AACACAGTGA AAATGTTCGA GAGGAGAGCT CCTCGTTTTA TGTGCAGGAG 
AGTGGGAGAC AGCTCCTGTT TCAGCCGCCC ACACTGGATT TTGGACCACA GCCGGTGGGG 
CTGCCAAGAG CTGAAACCGT ATATATCCAC AATCCCAGCC CAGAGCTCCC AGTCTCTCTG 
CTTTCTGTGT TTACATCCAG CAGACATTTT CACATGCCTT CCTTCCACAG AAGGGTAATT 
CCACCCAGAG GGAGAACATC TTTTAAAATC ATTTTCCTCC CCACAGTGGA GGGCAATGAA 
GAAATCTCTT TATTTATTAA TACATCAACC CATGGGTTGT TATCATACCA GGTTTTTGGG 
GTCGGCGTCC ATGGGGAATC AGTTAAGGAC GCCCACAGAA AAGATAGCGT TCTGATATTC 
CCCCACATAC AGAGCATTAA TCTAACACAA TCTCAGGAAG ATGCTTCTAA TATAACAATT 
TTAGGTCTGC TGCTTGACTG CAGTTTACCA AAAATGTTCT ACAATCAGCC CCAGGGTTGG 
TGTTTAGTAT CAGAGGAGGG CAGAATTGCT CTTCAGATCA GTCTTTCTGA AAGAGGGGAA 
AGGCCGACCC ACCTGGACAA ACTCAAGCCA TACGTGCTGG AGAATATTGT GGTGCTGCTG 
GTCCTCCCAC CACCAGAAGA TGCTGTCTCA GATCCCAAAA TAGGAGTGTT CATGTTGAAC 
TCAGGTGTTA AAAAGCTCTT TGTTAAGGAG ATCCAGCTTT TGTCGAGAAC AGACAGCAAT 
GTTGAACTCA CTGAAGTCTT GCTCAAGCCT TCTGCTACTA ACTTCACTCA AGTGGCTTCA 
CTCTTCTGTA GACATTCTTT GACGGCTAAA AGTAAAAGGT GTTCAAGTCA GTTCGGCCTA 
CACATTTTAG CAAACAGAAC AGTGAATTTA TATCCACTGC TGCAAAGAAC ACACAGATGG 
TCAGACTCTG AGCTGAGTCC TTTAATTCAG ATCAGACAGA AGCAGAGGGG CTCAGAGCAT 
GTGGAGCTCT GGCTAACAAA CCCTTTCCCC TTCTCCTTCA GAATCACTAA CATCTCCCTG 
TCCCAGCAGA AACTCTTTAA GAGTGTAAAT TTTACAACAT CTGTGGTAGT GAGCTCGGGG 
AGCTGGAAGC TTCTCTCTCT TCAGCTACTC GGTAAATCTT TACCCATCAA CCACAGAACC 
ACAATTATCC TGGCAACCAG TGTTGGGCTC CCACTAGAGC TTCACCTGCA AACATATTCT 
TCTTCTTCAA AGGGAGTTGT TATTGTTGAG TCTTCTGAGG AGTGTGAACG GCTATGTCCA 
CTCCGTATGT CCAGGGCAGG ACGTGTGGAG TGGCAGCAAT CGCTTCTCCC AGAATCATCC 
TCTTCACTAT GGAGAGTAGA CAGCAGTCTG GCCTCTGCCC TGTGCAGTCG TTGGCACTGT 
AGCAAAGAGC TCTCCTGCAG GTGGCCCAGA CTTCCTGAAG ATCACTCCAC ACCCCTAGAC 
TTTGGTGCCA CACCTGTCAA TGATAGTAAG GTAAAAAATT TCACTCTGAA AAACCCGACG 
GGTTCAGTGA TTTCTGTGGA GATACGAACC CTCTCCTCAT ACCCAGTTCC ACTTGAAGCA 
CTCGACATGC TTACCAAATG GTTTAATATT AGCCCCCTCT CTGTGAATAT CACTACAACA 
GAGTTTCTGC TGTTGGCTGC TAACCATAAG GAAAACAAGA GAGGTAGTGT GCTGAGAATC 
CTCCTTCAGC CGTGGGAGTC GAGGACTGTG TCTGTGGCGT ATCAACCCAC AGAACACAAG 
CCTGTTACCT CACTGCTCTT GATCAGGAAC AACCTGACCG TGTTTGACAT GGTTCTGGTT 
AAAGGATTAG GAGCAAAGGA GATGTTGAGG GTGGGAGGGA AGCTTCCTGG ACCTGCAGCC 
TCTCTGCGCT TCAATGTGCC TCAGTCCACC CTCATGGAGT GCAGAGATGG CTTACGATCA 
AGTAAGCCCT TATTTGCCAT CCAGAAAAGC TTTAAAGTGG AGAATGCAGG CGAGTTGCCG 
CTGACTGTAG TGTCCATGAA CATTAATGGA TATAAATGTC AGGGTTATGG TTTTGAAGTG 
CAGCACTGTG GATCCTTCAG AGTGGACTAC AACTCCTCCT CAGAAATCAC TATTGCATTT 
ACTCCAGACT TCACTTCCTC ATGGGTTATT AGAGACCTGA CGCTGGTGAC AGAGCGAGGC 
TCCTCTTTCC ATTTCACCCT TAATGTGACT CTTCCTCACC ACATGCTGCC GCTGTGTGCT 
CAGGTGGTCC CGGGACCCAG CTGGGAGGAG TCTTTCTGGA TCATCACACT GGCCTTTACC 
TGCTTCTCTC TGGCTGTTGT GTGTCTGATG GCTTTTCATC AAGCTCAACA CATTCTGAGT 
GAGTTCTCCA TGCCAAATCC ACGTAGCAAT CACAACTCGG TACCTCGAGA AAATAGCAGT 
GTTAACCACA GCTCTTCTAA TGGTGTCAGT CGAGGAAAAG GCAGTTGTAA GAACTACACT 
GACACAAGCC ACCCGTCTGA TAAAGGCAAA GGCAGAGGAT CTCCAGCTGT AGCCAATGGA 
TCAGTTCGGT TGCAGCCTTC ATCCAAGAAG AGCTCTGGAG GTTCTTCTCA GCCTCCAAAG 
AAGCAAACTA AGGTTTCATT ACTATACAGC AGATACAAAA ACAGCCCTGC GACTCTGGCT 
GCTAGTGTTT CTGCTACCAC TGACTTCTGT GGTTCGCCCA CCAATGAAGA TAAAGATCAC 
ACCTTTGAAT TAGACCCAGA AATTTGTAAT AACAACAACA ACAATAATGA TATATTAGAC 
GAAGCCCTTC TCCCTGTCGA GAAGAAAGAG CACTTTAGAG AATCCAAGGA AGAGAAGGTC 
TTGCCAGCTG TTATGTTTCC CATGGAAACA CTTCTTGGAT TACCTGAAAA CATCACAGCA 
AACAGAGTCC CTCAGCCTGC AGGCGAAGTG ATGGAAAATG AAGAATGTAA AAGAAAAACC 
AATGCAGATA AAAGGGATCA TCTTGATGAA CAGAAAACAG AAAGAGGCAG CAGTCAAAAG 
AAAAGAGCAG AGAAAGATGC AGAACTTGGG GTTTCTGCTC CTATCAACAA AACGAAGAAA 
AACGCAGGCA GGAGTCGCAA GAAGGCACCA GAGCTCATCC CTGGGCTTCC TGAAAACAGT 
GTTGTTATGG TGACAGAGAG TGAGAGAGAA CCATCACGTG CTGCTGCTCG AACACGAAAC 
GCCAAACCCG AACTTCCTAA AACAGGAGCA AACACAGATA GTCCTCTCAA ACACAGCGGC 
ACGTGTTTGG GCAGACCTAG GCGTAAGTGT CCAGAGCGGC GTCTGCAGTG GGAATCTGGC 
TCTGACTCCA GCAGTTCATC AGGCAGTGTG CGAGCCAGCC GAGGGTCCTG GGGCAGCTGG 
AGCAGTGCCA GCAGCATAGA GGGGGAAAGA GATCCAAACA GACACCCCTG CAGGAGAGAC 
AACATTCAAT ACAATCCATA TTTAAAAGAC CAGGAATGTT ACCTGAACAT GAACTATACC 
TGCAAAAATC CAAGCATGAA CAATCTTTAT GACAGAGAAC CATACCAAAG CTCAGAAATG 
GGTCCTGTCC CAGCACTTCC TCACACCTTC GCTGATGTAG CTGCAGGTGT GGACAAAAAC 
ATCGACGTAA TTGGCTCCAG CATGATGGAA GAGACGTGGT CAGTCAACTC CGTTCCTCTG 
ACCAATGAAT TCAGGTACAA CATGAGTGAA TCTCTCCCGT TTGTCCCTCA GAATCCCTCA 
TCCAGATATG ATGGCTTCCC TTGGAACAAC ATGAGCAGTC AGTGTAGCAG TCAGTATCCC 
TACAGTGACC GCAGCAACTA CATGCCAACA GGTAACGGCA ATTACCAAAA TGCATTCTCA 
TGCCCTGAAA GCCAAACCAT AAGCCACAGC CATCAGCCGG TGTGGGGTGA GAATGGAGCC 
CATGATATCA ACTCTACATG GGACACTGGC AGCTGTGTGG GCAGCAAGCC ATACTTTTCA 
GGAACCAGAA GTCTGTCCCC CATGTCCAGT CTATTTGGTT CAATCTGGAC ACCCCAAAGC 
GAGCCTTACC AGAGTCACTT CCACCCAGAG CGTTCAGCAC CAGTCTCGCC TCACACCCCC 
CATACACCCA TCTCACCGGT CGCCCCCTTC AGCCGTGAAC CCGGCGGCGC CTGTCCCGGG 
AAGCACTTCT CCAGCTTCAA CCCCTTCGGA CCACACATGA ATTTGGACAT ATGGAACTCC 
TCTTCAAACC GGAGCTCCAA CTCTCAGCTC TCCAATGATT CAGGCTACTG CGGTGACATG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-208H7.4 ( XM_009291318.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009289593.3 Danio rerio transmembrane 131 like (tmem131l), transcript variant X3, mRNA.

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CloneID	ODa44612
Clone ID Related Accession (Same CDS sequence)	XM_009291318.3
Accession Version	XM_009291318.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transmembrane protein 131-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291318.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCC AAAGGGACTT CACACACAGA AAAACATGGA TTAATGTTCT CCTGCGACTC 
TTACAGCTCT TATTGCTTTA CATTCAGCAC GGAGGAGCTC AGTTTCAAGC TCTGACTCAG 
ATGTCCAGCG TGGTCGAAGT TTGGCAGGCT GAGGACGCAG ACTTCTTCGT GCCATCTCAG 
GCAGAGGAGC AACACAGTGA AAATGTTCGA GAGGAGAGCT CCTCGTTTTA TGTGCAGGAG 
AGTGGGAGAC AGCTCCTGTT TCAGCCGCCC ACACTGGATT TTGGACCACA GCCGGTGGGG 
CTGCCAAGAG CTGAAACCGT ATATATCCAC AATCCCAGCC CAGAGCTCCC AGTCTCTCTG 
CTTTCTGTGT TTACATCCAG CAGACATTTT CACATGCCTT CCTTCCACAG AAGGGTAATT 
CCACCCAGAG GGAGAACATC TTTTAAAATC ATTTTCCTCC CCACAGTGGA GGGCAATGAA 
GAAATCTCTT TATTTATTAA TACATCAACC CATGGGTTGT TATCATACCA GGTTTTTGGG 
GTCGGCGTCC ATGGGGAATC AGTTAAGGAC GCCCACAGAA AAGATAGCGT TCTGATATTC 
CCCCACATAC AGAGCATTAA TCTAACACAA TCTCAGGAAG ATGCTTCTAA TATAACAATT 
TTAGGTCTGC TGCTTGACTG CAGTTTACCA AAAATGTTCT ACAATCAGCC CCAGGGTTGG 
TGTTTAGTAT CAGAGGAGGG CAGAATTGCT CTTCAGATCA GTCTTTCTGA AAGAGGGGAA 
AGGCCGACCC ACCTGGACAA ACTCAAGCCA TACGTGCTGG AGAATATTGT GGTGCTGCTG 
GTCCTCCCAC CACCAGAAGA TGCTGTCTAT CCCAAAATAG GAGTGTTCAT GTTGAACTCA 
GGTGTTAAAA AGCTCTTTGT TAAGGAGATC CAGCTTTTGT CGAGAACAGA CAGCAATGTT 
GAACTCACTG AAGTCTTGCT CAAGCCTTCT GCTACTAACT TCACTCAAGT GGCTTCACTC 
TTCTGTAGAC ATTCTTTGAC GGCTAAAAGT AAAAGGTGTT CAAGTCAGTT CGGCCTACAC 
ATTTTAGCAA ACAGAACAGT GAATTTATAT CCACTGCTGC AAAGAACACA CAGATGGTCA 
GACTCTGAGC TGAGTCCTTT AATTCAGATC AGACAGAAGC AGAGGGGCTC AGAGCATGTG 
GAGCTCTGGC TAACAAACCC TTTCCCCTTC TCCTTCAGAA TCACTAACAT CTCCCTGTCC 
CAGCAGAAAC TCTTTAAGAG TGTAAATTTT ACAACATCTG TGGTAGTGAG CTCGGGGAGC 
TGGAAGCTTC TCTCTCTTCA GCTACTCGGT AAATCTTTAC CCATCAACCA CAGAACCACA 
ATTATCCTGG CAACCAGTGT TGGGCTCCCA CTAGAGCTTC ACCTGCAAAC ATATTCTTCT 
TCTTCAAAGC AGGGAGTTGT TATTGTTGAG TCTTCTGAGG AGTGTGAACG GCTATGTCCA 
CTCCGTATGT CCAGGGCAGG ACGTGTGGAG TGGCAGCAAT CGCTTCTCCC AGAATCATCC 
TCTTCACTAT GGAGAGTAGA CAGCAGTCTG GCCTCTGCCC TGTGCAGTCG TTGGCACTGT 
AGCAAAGAGC TCTCCTGCAG GTGGCCCAGA CTTCCTGAAG ATCACTCCAC ACCCCTAGAC 
TTTGGTGCCA CACCTGTCAA TGATAGTAAG GTAAAAAATT TCACTCTGAA AAACCCGACG 
GGTTCAGTGA TTTCTGTGGA GATACGAACC CTCTCCTCAT ACCCAGTTCC ACTTGAAGCA 
CTCGACATGC TTACCAAATG GTTTAATATT AGCCCCCTCT CTGTGAATAT CACTACAACA 
GAGTTTCTGC TGTTGGCTGC TAACCATAAG GAAAACAAGA GAGGTAGTGT GCTGAGAATC 
CTCCTTCAGC CGTGGGAGTC GAGGACTGTG TCTGTGGCGT ATCAACCCAC AGAACACAAG 
CCTGTTACCT CACTGCTCTT GATCAGGAAC AACCTGACCG TGTTTGACAT GGTTCTGGTT 
AAAGGATTAG GAGCAAAGGA GATGTTGAGG GTGGGAGGGA AGCTTCCTGG ACCTGCAGCC 
TCTCTGCGCT TCAATGTGCC TCAGTCCACC CTCATGGAGT GCAGAGATGG CTTACGATCA 
AGTAAGCCCT TATTTGCCAT CCAGAAAAGC TTTAAAGTGG AGAATGCAGG CGAGTTGCCG 
CTGACTGTAG TGTCCATGAA CATTAATGGA TATAAATGTC AGGGTTATGG TTTTGAAGTG 
CAGCACTGTG GATCCTTCAG AGTGGACTAC AACTCCTCCT CAGAAATCAC TATTGCATTT 
ACTCCAGACT TCACTTCCTC ATGGGTTATT AGAGACCTGA CGCTGGTGAC AGAGCGAGGC 
TCCTCTTTCC ATTTCACCCT TAATGTGACT CTTCCTCACC ACATGCTGCC GCTGTGTGCT 
CAGGTGGTCC CGGGACCCAG CTGGGAGGAG TCTTTCTGGA TCATCACACT GGCCTTTACC 
TGCTTCTCTC TGGCTGTTGT GTGTCTGATG GCTTTTCATC AAGCTCAACA CATTCTGAGT 
GAGTTCTCCA TGCCAAATCC ACGTAGCAAT CACAACTCGG TACCTCGAGA AAATAGCAGT 
GTTAACCACA GCTCTTCTAA TGGTGTCAGT CGAGGAAAAG GCAGTTGTAA GAACTACACT 
GACACAAGCC ACCCGTCTGA TAAAGGCAAA GGCAGAGGAT CTCCAGCTGT AGCCAATGGA 
TCAGTTCGGT TGCAGCCTTC ATCCAAGAAG AGCTCTGGAG GTTCTTCTCA GCCTCCAAAG 
AAGCAAACTA AGGTTTCATT ACTATACAGC AGATACAAAA ACAGCCCTGC GACTCTGGCT 
GCTAGTGTTT CTGCTACCAC TGACTTCTGT GGTTCGCCCA CCAATGAAGA TAAAGATCAC 
ACCTTTGAAT TAGACCCAGA AATTTGTAAT AACAACAACA ACAATAATGA TATATTAGAC 
GAAGCCCTTC TCCCTGTCGA GAAGAAAGAG CACTTTAGAG AATCCAAGGA AGAGAAGGTC 
TTGCCAGCTG TTATGTTTCC CATGGAAACA CTTCTTGGAT TACCTGAAAA CATCACAGCA 
AACAGAGTCC CTCAGCCTGC AGGCGAAGTG ATGGAAAATG AAGAATGTAA AAGAAAAACC 
AATGCAGATA AAAGGGATCA TCTTGATGAA CAGAAAACAG AAAGAGGCAG CAGTCAAAAG 
AAAAGAGCAG AGAAAGATGC AGAACTTGGG GTTTCTGCTC CTATCAACAA AACGAAGAAA 
AACGCAGGCA GGAGTCGCAA GAAGGCACCA GAGCTCATCC CTGGGCTTCC TGAAAACAGT 
GTTGTTATGG TGACAGAGAG TGAGAGAGAA CCATCACGTG CTGCTGCTCG AACACGAAAC 
GCCAAACCCG AACTTCCTAA AACAGGAGCA AACACAGATA GTCCTCTCAA ACACAGCGGC 
ACGTGTTTGG GCAGACCTAG GCGTAAGTGT CCAGAGCGGC GTCTGCAGTG GGAATCTGGC 
TCTGACTCCA GCAGTTCATC AGGCAGTGTG CGAGCCAGCC GAGGGTCCTG GGGCAGCTGG 
AGCAGTGCCA GCAGCATAGA GGGGGAAAGA GATCCAAACA GACACCCCTG CAGGAGAGAC 
AACATTCAAT ACAATCCATA TTTAAAAGAC CAGGAATGTT ACCTGAACAT GAACTATACC 
TGCAAAAATC CAAGCATGAA CAATCTTTAT GACAGAGAAC CATACCAAAG CTCAGAAATG 
GGTCCTGTCC CAGCACTTCC TCACACCTTC GCTGATGTAG CTGCAGGTGT GGACAAAAAC 
ATCGACGTAA TTGGCTCCAG CATGATGGAA GAGACGTGGT CAGTCAACTC CGTTCCTCTG 
ACCAATGAAT TCAGGTACAA CATGAGTGAA TCTCTCCCGT TTGTCCCTCA GAATCCCTCA 
TCCAGATATG ATGGCTTCCC TTGGAACAAC ATGAGCAGTC AGTGTAGCAG TCAGTATCCC 
TACAGTGACC GCAGCAACTA CATGCCAACA GGTAACGGCA ATTACCAAAA TGCATTCTCA 
TGCCCTGAAA GCCAAACCAT AAGCCACAGC CATCAGCCGG TGTGGGGTGA GAATGGAGCC 
CATGATATCA ACTCTACATG GGACACTGGC AGCTGTGTGG GCAGCAAGCC ATACTTTTCA 
GGAACCAGAA GTCTGTCCCC CATGTCCAGT CTATTTGGTT CAATCTGGAC ACCCCAAAGC 
GAGCCTTACC AGAGTCACTT CCACCCAGAG CGTTCAGCAC CAGTCTCGCC TCACACCCCC 
CATACACCCA TCTCACCGGT CGCCCCCTTC AGCCGTGAAC CCGGCGGCGC CTGTCCCGGG 
AAGCACTTCT CCAGCTTCAA CCCCTTCGGA CCACACATGA ATTTGGACAT ATGGAACTCC 
TCTTCAAACC GGAGCTCCAA CTCTCAGCTC TCCAATGATT CAGGCTACTG CGGTGACATG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289593.3
CDS	126..4628
Translation	MASQRDFTHRKTWINVLLRLLQLLLLYIQHGGAQFQALTQMSSVVEVWQAEDADFFVPSQAEEQHSENVREESSSFYVQESGRQLLFQPPTLDFGPQPVGLPRAETVYIHNPSPELPVSLLSVFTSSRHFHMPSFHRRVIPPRGRTSFKIIFLPTVEGNEEISLFINTSTHGLLSYQVFGVGVHGESVKDAHRKDSVLIFPHIQSINLTQSQEDASNITILGLLLDCSLPKMFYNQPQGWCLVSEEGRIALQISLSERGERPTHLDKLKPYVLENIVVLLVLPPPEDAVYPKIGVFMLNSGVKKLFVKEIQLLSRTDSNVELTEVLLKPSATNFTQVASLFCRHSLTAKSKRCSSQFGLHILANRTVNLYPLLQRTHRWSDSELSPLIQIRQKQRGSEHVELWLTNPFPFSFRITNISLSQQKLFKSVNFTTSVVVSSGSWKLLSLQLLGKSLPINHRTTIILATSVGLPLELHLQTYSSSSKQGVVIVESSEECERLCPLRMSRAGRVEWQQSLLPESSSSLWRVDSSLASALCSRWHCSKELSCRWPRLPEDHSTPLDFGATPVNDSKVKNFTLKNPTGSVISVEIRTLSSYPVPLEALDMLTKWFNISPLSVNITTTEFLLLAANHKENKRGSVLRILLQPWESRTVSVAYQPTEHKPVTSLLLIRNNLTVFDMVLVKGLGAKEMLRVGGKLPGPAASLRFNVPQSTLMECRDGLRSSKPLFAIQKSFKVENAGELPLTVVSMNINGYKCQGYGFEVQHCGSFRVDYNSSSEITIAFTPDFTSSWVIRDLTLVTERGSSFHFTLNVTLPHHMLPLCAQVVPGPSWEESFWIITLAFTCFSLAVVCLMAFHQAQHILSEFSMPNPRSNHNSVPRENSSVNHSSSNGVSRGKGSCKNYTDTSHPSDKGKGRGSPAVANGSVRLQPSSKKSSGGSSQPPKKQTKVSLLYSRYKNSPATLAASVSATTDFCGSPTNEDKDHTFELDPEICNNNNNNNDILDEALLPVEKKEHFRESKEEKVLPAVMFPMETLLGLPENITANRVPQPAGEVMENEECKRKTNADKRDHLDEQKTERGSSQKKRAEKDAELGVSAPINKTKKNAGRSRKKAPELIPGLPENSVVMVTESEREPSRAAARTRNAKPELPKTGANTDSPLKHSGTCLGRPRRKCPERRLQWESGSDSSSSSGSVRASRGSWGSWSSASSIEGERDPNRHPCRRDNIQYNPYLKDQECYLNMNYTCKNPSMNNLYDREPYQSSEMGPVPALPHTFADVAAGVDKNIDVIGSSMMEETWSVNSVPLTNEFRYNMSESLPFVPQNPSSRYDGFPWNNMSSQCSSQYPYSDRSNYMPTGNGNYQNAFSCPESQTISHSHQPVWGENGAHDINSTWDTGSCVGSKPYFSGTRSLSPMSSLFGSIWTPQSEPYQSHFHPERSAPVSPHTPHTPISPVAPFSREPGGACPGKHFSSFNPFGPHMNLDIWNSSSNRSSNSQLSNDSGYCGDM

Target ORF information:

RefSeq Version	XM_009291318.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transmembrane 131 like (tmem131l), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291318.3

ORF Insert Sequence:

ATGGCGAGCC AAAGGGACTT CACACACAGA AAAACATGGA TTAATGTTCT CCTGCGACTC 
TTACAGCTCT TATTGCTTTA CATTCAGCAC GGAGGAGCTC AGTTTCAAGC TCTGACTCAG 
ATGTCCAGCG TGGTCGAAGT TTGGCAGGCT GAGGACGCAG ACTTCTTCGT GCCATCTCAG 
GCAGAGGAGC AACACAGTGA AAATGTTCGA GAGGAGAGCT CCTCGTTTTA TGTGCAGGAG 
AGTGGGAGAC AGCTCCTGTT TCAGCCGCCC ACACTGGATT TTGGACCACA GCCGGTGGGG 
CTGCCAAGAG CTGAAACCGT ATATATCCAC AATCCCAGCC CAGAGCTCCC AGTCTCTCTG 
CTTTCTGTGT TTACATCCAG CAGACATTTT CACATGCCTT CCTTCCACAG AAGGGTAATT 
CCACCCAGAG GGAGAACATC TTTTAAAATC ATTTTCCTCC CCACAGTGGA GGGCAATGAA 
GAAATCTCTT TATTTATTAA TACATCAACC CATGGGTTGT TATCATACCA GGTTTTTGGG 
GTCGGCGTCC ATGGGGAATC AGTTAAGGAC GCCCACAGAA AAGATAGCGT TCTGATATTC 
CCCCACATAC AGAGCATTAA TCTAACACAA TCTCAGGAAG ATGCTTCTAA TATAACAATT 
TTAGGTCTGC TGCTTGACTG CAGTTTACCA AAAATGTTCT ACAATCAGCC CCAGGGTTGG 
TGTTTAGTAT CAGAGGAGGG CAGAATTGCT CTTCAGATCA GTCTTTCTGA AAGAGGGGAA 
AGGCCGACCC ACCTGGACAA ACTCAAGCCA TACGTGCTGG AGAATATTGT GGTGCTGCTG 
GTCCTCCCAC CACCAGAAGA TGCTGTCTAT CCCAAAATAG GAGTGTTCAT GTTGAACTCA 
GGTGTTAAAA AGCTCTTTGT TAAGGAGATC CAGCTTTTGT CGAGAACAGA CAGCAATGTT 
GAACTCACTG AAGTCTTGCT CAAGCCTTCT GCTACTAACT TCACTCAAGT GGCTTCACTC 
TTCTGTAGAC ATTCTTTGAC GGCTAAAAGT AAAAGGTGTT CAAGTCAGTT CGGCCTACAC 
ATTTTAGCAA ACAGAACAGT GAATTTATAT CCACTGCTGC AAAGAACACA CAGATGGTCA 
GACTCTGAGC TGAGTCCTTT AATTCAGATC AGACAGAAGC AGAGGGGCTC AGAGCATGTG 
GAGCTCTGGC TAACAAACCC TTTCCCCTTC TCCTTCAGAA TCACTAACAT CTCCCTGTCC 
CAGCAGAAAC TCTTTAAGAG TGTAAATTTT ACAACATCTG TGGTAGTGAG CTCGGGGAGC 
TGGAAGCTTC TCTCTCTTCA GCTACTCGGT AAATCTTTAC CCATCAACCA CAGAACCACA 
ATTATCCTGG CAACCAGTGT TGGGCTCCCA CTAGAGCTTC ACCTGCAAAC ATATTCTTCT 
TCTTCAAAGC AGGGAGTTGT TATTGTTGAG TCTTCTGAGG AGTGTGAACG GCTATGTCCA 
CTCCGTATGT CCAGGGCAGG ACGTGTGGAG TGGCAGCAAT CGCTTCTCCC AGAATCATCC 
TCTTCACTAT GGAGAGTAGA CAGCAGTCTG GCCTCTGCCC TGTGCAGTCG TTGGCACTGT 
AGCAAAGAGC TCTCCTGCAG GTGGCCCAGA CTTCCTGAAG ATCACTCCAC ACCCCTAGAC 
TTTGGTGCCA CACCTGTCAA TGATAGTAAG GTAAAAAATT TCACTCTGAA AAACCCGACG 
GGTTCAGTGA TTTCTGTGGA GATACGAACC CTCTCCTCAT ACCCAGTTCC ACTTGAAGCA 
CTCGACATGC TTACCAAATG GTTTAATATT AGCCCCCTCT CTGTGAATAT CACTACAACA 
GAGTTTCTGC TGTTGGCTGC TAACCATAAG GAAAACAAGA GAGGTAGTGT GCTGAGAATC 
CTCCTTCAGC CGTGGGAGTC GAGGACTGTG TCTGTGGCGT ATCAACCCAC AGAACACAAG 
CCTGTTACCT CACTGCTCTT GATCAGGAAC AACCTGACCG TGTTTGACAT GGTTCTGGTT 
AAAGGATTAG GAGCAAAGGA GATGTTGAGG GTGGGAGGGA AGCTTCCTGG ACCTGCAGCC 
TCTCTGCGCT TCAATGTGCC TCAGTCCACC CTCATGGAGT GCAGAGATGG CTTACGATCA 
AGTAAGCCCT TATTTGCCAT CCAGAAAAGC TTTAAAGTGG AGAATGCAGG CGAGTTGCCG 
CTGACTGTAG TGTCCATGAA CATTAATGGA TATAAATGTC AGGGTTATGG TTTTGAAGTG 
CAGCACTGTG GATCCTTCAG AGTGGACTAC AACTCCTCCT CAGAAATCAC TATTGCATTT 
ACTCCAGACT TCACTTCCTC ATGGGTTATT AGAGACCTGA CGCTGGTGAC AGAGCGAGGC 
TCCTCTTTCC ATTTCACCCT TAATGTGACT CTTCCTCACC ACATGCTGCC GCTGTGTGCT 
CAGGTGGTCC CGGGACCCAG CTGGGAGGAG TCTTTCTGGA TCATCACACT GGCCTTTACC 
TGCTTCTCTC TGGCTGTTGT GTGTCTGATG GCTTTTCATC AAGCTCAACA CATTCTGAGT 
GAGTTCTCCA TGCCAAATCC ACGTAGCAAT CACAACTCGG TACCTCGAGA AAATAGCAGT 
GTTAACCACA GCTCTTCTAA TGGTGTCAGT CGAGGAAAAG GCAGTTGTAA GAACTACACT 
GACACAAGCC ACCCGTCTGA TAAAGGCAAA GGCAGAGGAT CTCCAGCTGT AGCCAATGGA 
TCAGTTCGGT TGCAGCCTTC ATCCAAGAAG AGCTCTGGAG GTTCTTCTCA GCCTCCAAAG 
AAGCAAACTA AGGTTTCATT ACTATACAGC AGATACAAAA ACAGCCCTGC GACTCTGGCT 
GCTAGTGTTT CTGCTACCAC TGACTTCTGT GGTTCGCCCA CCAATGAAGA TAAAGATCAC 
ACCTTTGAAT TAGACCCAGA AATTTGTAAT AACAACAACA ACAATAATGA TATATTAGAC 
GAAGCCCTTC TCCCTGTCGA GAAGAAAGAG CACTTTAGAG AATCCAAGGA AGAGAAGGTC 
TTGCCAGCTG TTATGTTTCC CATGGAAACA CTTCTTGGAT TACCTGAAAA CATCACAGCA 
AACAGAGTCC CTCAGCCTGC AGGCGAAGTG ATGGAAAATG AAGAATGTAA AAGAAAAACC 
AATGCAGATA AAAGGGATCA TCTTGATGAA CAGAAAACAG AAAGAGGCAG CAGTCAAAAG 
AAAAGAGCAG AGAAAGATGC AGAACTTGGG GTTTCTGCTC CTATCAACAA AACGAAGAAA 
AACGCAGGCA GGAGTCGCAA GAAGGCACCA GAGCTCATCC CTGGGCTTCC TGAAAACAGT 
GTTGTTATGG TGACAGAGAG TGAGAGAGAA CCATCACGTG CTGCTGCTCG AACACGAAAC 
GCCAAACCCG AACTTCCTAA AACAGGAGCA AACACAGATA GTCCTCTCAA ACACAGCGGC 
ACGTGTTTGG GCAGACCTAG GCGTAAGTGT CCAGAGCGGC GTCTGCAGTG GGAATCTGGC 
TCTGACTCCA GCAGTTCATC AGGCAGTGTG CGAGCCAGCC GAGGGTCCTG GGGCAGCTGG 
AGCAGTGCCA GCAGCATAGA GGGGGAAAGA GATCCAAACA GACACCCCTG CAGGAGAGAC 
AACATTCAAT ACAATCCATA TTTAAAAGAC CAGGAATGTT ACCTGAACAT GAACTATACC 
TGCAAAAATC CAAGCATGAA CAATCTTTAT GACAGAGAAC CATACCAAAG CTCAGAAATG 
GGTCCTGTCC CAGCACTTCC TCACACCTTC GCTGATGTAG CTGCAGGTGT GGACAAAAAC 
ATCGACGTAA TTGGCTCCAG CATGATGGAA GAGACGTGGT CAGTCAACTC CGTTCCTCTG 
ACCAATGAAT TCAGGTACAA CATGAGTGAA TCTCTCCCGT TTGTCCCTCA GAATCCCTCA 
TCCAGATATG ATGGCTTCCC TTGGAACAAC ATGAGCAGTC AGTGTAGCAG TCAGTATCCC 
TACAGTGACC GCAGCAACTA CATGCCAACA GGTAACGGCA ATTACCAAAA TGCATTCTCA 
TGCCCTGAAA GCCAAACCAT AAGCCACAGC CATCAGCCGG TGTGGGGTGA GAATGGAGCC 
CATGATATCA ACTCTACATG GGACACTGGC AGCTGTGTGG GCAGCAAGCC ATACTTTTCA 
GGAACCAGAA GTCTGTCCCC CATGTCCAGT CTATTTGGTT CAATCTGGAC ACCCCAAAGC 
GAGCCTTACC AGAGTCACTT CCACCCAGAG CGTTCAGCAC CAGTCTCGCC TCACACCCCC 
CATACACCCA TCTCACCGGT CGCCCCCTTC AGCCGTGAAC CCGGCGGCGC CTGTCCCGGG 
AAGCACTTCT CCAGCTTCAA CCCCTTCGGA CCACACATGA ATTTGGACAT ATGGAACTCC 
TCTTCAAACC GGAGCTCCAA CTCTCAGCTC TCCAATGATT CAGGCTACTG CGGTGACATG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-208H7.4 ( XM_688724.7 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_693816.7 Danio rerio si:ch211-208h7.4 (si:ch211-208h7.4), transcript variant X1, mRNA.

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CloneID	ODa53886
Clone ID Related Accession (Same CDS sequence)	XM_688724.7
Accession Version	XM_688724.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4641bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	transmembrane protein 131-like isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_688724.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATTGTC GGGCTCTAGT GGGCGCACAC ACTCGCAGAG GTTCTGCCGA AAGAGAGACA 
GACAGAGACA GAGGAGGGAG GGTGAGAGAG AGAGAAAGAG AAAGAAGTGA AGCCAGGGAG 
CTCAACACAG CCAGCATGGC GAGCCAAAGG GACTTCACAC ACAGAAAAAC ATGGATTAAT 
GTTCTCCTGC GACTCTTACA GCTCTTATTG CTTTACATTC AGCACGGAGG AGCTCAGTTT 
CAAGCTCTGA CTCAGATGTC CAGCGTGGTC GAAGTTTGGC AGGCTGAGGA CGCAGACTTC 
TTCGTGCCAT CTCAGGCAGA GGAGCAACAC AGTGAAAATG TTCGAGAGGA GAGCTCCTCG 
TTTTATGTGC AGGAGAGTGG GAGACAGCTC CTGTTTCAGC CGCCCACACT GGATTTTGGA 
CCACAGCCGG TGGGGCTGCC AAGAGCTGAA ACCGTATATA TCCACAATCC CAGCCCAGAG 
CTCCCAGTCT CTCTGCTTTC TGTGTTTACA TCCAGCAGAC ATTTTCACAT GCCTTCCTTC 
CACAGAAGGG TAATTCCACC CAGAGGGAGA ACATCTTTTA AAATCATTTT CCTCCCCACA 
GTGGAGGGCA ATGAAGAAAT CTCTTTATTT ATTAATACAT CAACCCATGG GTTGTTATCA 
TACCAGGTTT TTGGGGTCGG CGTCCATGGG GAATCAGTTA AGGACGCCCA CAGAAAAGAT 
AGCGTTCTGA TATTCCCCCA CATACAGAGC ATTAATCTAA CACAATCTCA GGAAGATGCT 
TCTAATATAA CAATTTTAGG TCTGCTGCTT GACTGCAGTT TACCAAAAAT GTTCTACAAT 
CAGCCCCAGG GTTGGTGTTT AGTATCAGAG GAGGGCAGAA TTGCTCTTCA GATCAGTCTT 
TCTGAAAGAG GGGAAAGGCC GACCCACCTG GACAAACTCA AGCCATACGT GCTGGAGAAT 
ATTGTGGTGC TGCTGGTCCT CCCACCACCA GAAGATGCTG TCTCAGATCC CAAAATAGGA 
GTGTTCATGT TGAACTCAGG TGTTAAAAAG CTCTTTGTTA AGGAGATCCA GCTTTTGTCG 
AGAACAGACA GCAATGTTGA ACTCACTGAA GTCTTGCTCA AGCCTTCTGC TACTAACTTC 
ACTCAAGTGG CTTCACTCTT CTGTAGACAT TCTTTGACGG CTAAAAGTAA AAGGTGTTCA 
AGTCAGTTCG GCCTACACAT TTTAGCAAAC AGAACAGTGA ATTTATATCC ACTGCTGCAA 
AGAACACACA GATGGTCAGA CTCTGAGCTG AGTCCTTTAA TTCAGATCAG ACAGAAGCAG 
AGGGGCTCAG AGCATGTGGA GCTCTGGCTA ACAAACCCTT TCCCCTTCTC CTTCAGAATC 
ACTAACATCT CCCTGTCCCA GCAGAAACTC TTTAAGAGTG TAAATTTTAC AACATCTGTG 
GTAGTGAGCT CGGGGAGCTG GAAGCTTCTC TCTCTTCAGC TACTCGGTAA ATCTTTACCC 
ATCAACCACA GAACCACAAT TATCCTGGCA ACCAGTGTTG GGCTCCCACT AGAGCTTCAC 
CTGCAAACAT ATTCTTCTTC TTCAAAGCAG GGAGTTGTTA TTGTTGAGTC TTCTGAGGAG 
TGTGAACGGC TATGTCCACT CCGTATGTCC AGGGCAGGAC GTGTGGAGTG GCAGCAATCG 
CTTCTCCCAG AATCATCCTC TTCACTATGG AGAGTAGACA GCAGTCTGGC CTCTGCCCTG 
TGCAGTCGTT GGCACTGTAG CAAAGAGCTC TCCTGCAGGT GGCCCAGACT TCCTGAAGAT 
CACTCCACAC CCCTAGACTT TGGTGCCACA CCTGTCAATG ATAGTAAGGT AAAAAATTTC 
ACTCTGAAAA ACCCGACGGG TTCAGTGATT TCTGTGGAGA TACGAACCCT CTCCTCATAC 
CCAGTTCCAC TTGAAGCACT CGACATGCTT ACCAAATGGT TTAATATTAG CCCCCTCTCT 
GTGAATATCA CTACAACAGA GTTTCTGCTG TTGGCTGCTA ACCATAAGGA AAACAAGAGA 
GGTAGTGTGC TGAGAATCCT CCTTCAGCCG TGGGAGTCGA GGACTGTGTC TGTGGCGTAT 
CAACCCACAG AACACAAGCC TGTTACCTCA CTGCTCTTGA TCAGGAACAA CCTGACCGTG 
TTTGACATGG TTCTGGTTAA AGGATTAGGA GCAAAGGAGA TGTTGAGGGT GGGAGGGAAG 
CTTCCTGGAC CTGCAGCCTC TCTGCGCTTC AATGTGCCTC AGTCCACCCT CATGGAGTGC 
AGAGATGGCT TACGATCAAG TAAGCCCTTA TTTGCCATCC AGAAAAGCTT TAAAGTGGAG 
AATGCAGGCG AGTTGCCGCT GACTGTAGTG TCCATGAACA TTAATGGATA TAAATGTCAG 
GGTTATGGTT TTGAAGTGCA GCACTGTGGA TCCTTCAGAG TGGACTACAA CTCCTCCTCA 
GAAATCACTA TTGCATTTAC TCCAGACTTC ACTTCCTCAT GGGTTATTAG AGACCTGACG 
CTGGTGACAG AGCGAGGCTC CTCTTTCCAT TTCACCCTTA ATGTGACTCT TCCTCACCAC 
ATGCTGCCGC TGTGTGCTCA GGTGGTCCCG GGACCCAGCT GGGAGGAGTC TTTCTGGATC 
ATCACACTGG CCTTTACCTG CTTCTCTCTG GCTGTTGTGT GTCTGATGGC TTTTCATCAA 
GCTCAACACA TTCTGAGTGA GTTCTCCATG CCAAATCCAC GTAGCAATCA CAACTCGGTA 
CCTCGAGAAA ATAGCAGTGT TAACCACAGC TCTTCTAATG GTGTCAGTCG AGGAAAAGGC 
AGTTGTAAGA ACTACACTGA CACAAGCCAC CCGTCTGATA AAGGCAAAGG CAGAGGATCT 
CCAGCTGTAG CCAATGGATC AGTTCGGTTG CAGCCTTCAT CCAAGAAGAG CTCTGGAGGT 
TCTTCTCAGC CTCCAAAGAA GCAAACTAAG GTTTCATTAC TATACAGCAG ATACAAAAAC 
AGCCCTGCGA CTCTGGCTGC TAGTGTTTCT GCTACCACTG ACTTCTGTGG TTCGCCCACC 
AATGAAGATA AAGATCACAC CTTTGAATTA GACCCAGAAA TTTGTAATAA CAACAACAAC 
AATAATGATA TATTAGACGA AGCCCTTCTC CCTGTCGAGA AGAAAGAGCA CTTTAGAGAA 
TCCAAGGAAG AGAAGGTCTT GCCAGCTGTT ATGTTTCCCA TGGAAACACT TCTTGGATTA 
CCTGAAAACA TCACAGCAAA CAGAGTCCCT CAGCCTGCAG GCGAAGTGAT GGAAAATGAA 
GAATGTAAAA GAAAAACCAA TGCAGATAAA AGGGATCATC TTGATGAACA GAAAACAGAA 
AGAGGCAGCA GTCAAAAGAA AAGAGCAGAG AAAGATGCAG AACTTGGGGT TTCTGCTCCT 
ATCAACAAAA CGAAGAAAAA CGCAGGCAGG AGTCGCAAGA AGGCACCAGA GCTCATCCCT 
GGGCTTCCTG AAAACAGTGT TGTTATGGTG ACAGAGAGTG AGAGAGAACC ATCACGTGCT 
GCTGCTCGAA CACGAAACGC CAAACCCGAA CTTCCTAAAA CAGGAGCAAA CACAGATAGT 
CCTCTCAAAC ACAGCGGCAC GTGTTTGGGC AGACCTAGGC GTAAGTGTCC AGAGCGGCGT 
CTGCAGTGGG AATCTGGCTC TGACTCCAGC AGTTCATCAG GCAGTGTGCG AGCCAGCCGA 
GGGTCCTGGG GCAGCTGGAG CAGTGCCAGC AGCATAGAGG GGGAAAGAGA TCCAAACAGA 
CACCCCTGCA GGAGAGACAA CATTCAATAC AATCCATATT TAAAAGACCA GGAATGTTAC 
CTGAACATGA ACTATACCTG CAAAAATCCA AGCATGAACA ATCTTTATGA CAGAGAACCA 
TACCAAAGCT CAGAAATGGG TCCTGTCCCA GCACTTCCTC ACACCTTCGC TGATGTAGCT 
GCAGGTGTGG ACAAAAACAT CGACGTAATT GGCTCCAGCA TGATGGAAGA GACGTGGTCA 
GTCAACTCCG TTCCTCTGAC CAATGAATTC AGGTACAACA TGAGTGAATC TCTCCCGTTT 
GTCCCTCAGA ATCCCTCATC CAGATATGAT GGCTTCCCTT GGAACAACAT GAGCAGTCAG 
TGTAGCAGTC AGTATCCCTA CAGTGACCGC AGCAACTACA TGCCAACAGG TAACGGCAAT 
TACCAAAATG CATTCTCATG CCCTGAAAGC CAAACCATAA GCCACAGCCA TCAGCCGGTG 
TGGGGTGAGA ATGGAGCCCA TGATATCAAC TCTACATGGG ACACTGGCAG CTGTGTGGGC 
AGCAAGCCAT ACTTTTCAGG AACCAGAAGT CTGTCCCCCA TGTCCAGTCT ATTTGGTTCA 
ATCTGGACAC CCCAAAGCGA GCCTTACCAG AGTCACTTCC ACCCAGAGCG TTCAGCACCA 
GTCTCGCCTC ACACCCCCCA TACACCCATC TCACCGGTCG CCCCCTTCAG CCGTGAACCC 
GGCGGCGCCT GTCCCGGGAA GCACTTCTCC AGCTTCAACC CCTTCGGACC ACACATGAAT 
TTGGACATAT GGAACTCCTC TTCAAACCGG AGCTCCAACT CTCAGCTCTC CAATGATTCA 
GGCTACTGCG GTGACATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_693816.7
CDS	35..4675
Translation	MYCRALVGAHTRRGSAERETDRDRGGRVRERERERSEARELNTASMASQRDFTHRKTWINVLLRLLQLLLLYIQHGGAQFQALTQMSSVVEVWQAEDADFFVPSQAEEQHSENVREESSSFYVQESGRQLLFQPPTLDFGPQPVGLPRAETVYIHNPSPELPVSLLSVFTSSRHFHMPSFHRRVIPPRGRTSFKIIFLPTVEGNEEISLFINTSTHGLLSYQVFGVGVHGESVKDAHRKDSVLIFPHIQSINLTQSQEDASNITILGLLLDCSLPKMFYNQPQGWCLVSEEGRIALQISLSERGERPTHLDKLKPYVLENIVVLLVLPPPEDAVSDPKIGVFMLNSGVKKLFVKEIQLLSRTDSNVELTEVLLKPSATNFTQVASLFCRHSLTAKSKRCSSQFGLHILANRTVNLYPLLQRTHRWSDSELSPLIQIRQKQRGSEHVELWLTNPFPFSFRITNISLSQQKLFKSVNFTTSVVVSSGSWKLLSLQLLGKSLPINHRTTIILATSVGLPLELHLQTYSSSSKQGVVIVESSEECERLCPLRMSRAGRVEWQQSLLPESSSSLWRVDSSLASALCSRWHCSKELSCRWPRLPEDHSTPLDFGATPVNDSKVKNFTLKNPTGSVISVEIRTLSSYPVPLEALDMLTKWFNISPLSVNITTTEFLLLAANHKENKRGSVLRILLQPWESRTVSVAYQPTEHKPVTSLLLIRNNLTVFDMVLVKGLGAKEMLRVGGKLPGPAASLRFNVPQSTLMECRDGLRSSKPLFAIQKSFKVENAGELPLTVVSMNINGYKCQGYGFEVQHCGSFRVDYNSSSEITIAFTPDFTSSWVIRDLTLVTERGSSFHFTLNVTLPHHMLPLCAQVVPGPSWEESFWIITLAFTCFSLAVVCLMAFHQAQHILSEFSMPNPRSNHNSVPRENSSVNHSSSNGVSRGKGSCKNYTDTSHPSDKGKGRGSPAVANGSVRLQPSSKKSSGGSSQPPKKQTKVSLLYSRYKNSPATLAASVSATTDFCGSPTNEDKDHTFELDPEICNNNNNNNDILDEALLPVEKKEHFRESKEEKVLPAVMFPMETLLGLPENITANRVPQPAGEVMENEECKRKTNADKRDHLDEQKTERGSSQKKRAEKDAELGVSAPINKTKKNAGRSRKKAPELIPGLPENSVVMVTESEREPSRAAARTRNAKPELPKTGANTDSPLKHSGTCLGRPRRKCPERRLQWESGSDSSSSSGSVRASRGSWGSWSSASSIEGERDPNRHPCRRDNIQYNPYLKDQECYLNMNYTCKNPSMNNLYDREPYQSSEMGPVPALPHTFADVAAGVDKNIDVIGSSMMEETWSVNSVPLTNEFRYNMSESLPFVPQNPSSRYDGFPWNNMSSQCSSQYPYSDRSNYMPTGNGNYQNAFSCPESQTISHSHQPVWGENGAHDINSTWDTGSCVGSKPYFSGTRSLSPMSSLFGSIWTPQSEPYQSHFHPERSAPVSPHTPHTPISPVAPFSREPGGACPGKHFSSFNPFGPHMNLDIWNSSSNRSSNSQLSNDSGYCGDM

Target ORF information:

RefSeq Version	XM_688724.7
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-208h7.4 (si:ch211-208h7.4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_688724.7

ORF Insert Sequence:

ATGTATTGTC GGGCTCTAGT GGGCGCACAC ACTCGCAGAG GTTCTGCCGA AAGAGAGACA 
GACAGAGACA GAGGAGGGAG GGTGAGAGAG AGAGAAAGAG AAAGAAGTGA AGCCAGGGAG 
CTCAACACAG CCAGCATGGC GAGCCAAAGG GACTTCACAC ACAGAAAAAC ATGGATTAAT 
GTTCTCCTGC GACTCTTACA GCTCTTATTG CTTTACATTC AGCACGGAGG AGCTCAGTTT 
CAAGCTCTGA CTCAGATGTC CAGCGTGGTC GAAGTTTGGC AGGCTGAGGA CGCAGACTTC 
TTCGTGCCAT CTCAGGCAGA GGAGCAACAC AGTGAAAATG TTCGAGAGGA GAGCTCCTCG 
TTTTATGTGC AGGAGAGTGG GAGACAGCTC CTGTTTCAGC CGCCCACACT GGATTTTGGA 
CCACAGCCGG TGGGGCTGCC AAGAGCTGAA ACCGTATATA TCCACAATCC CAGCCCAGAG 
CTCCCAGTCT CTCTGCTTTC TGTGTTTACA TCCAGCAGAC ATTTTCACAT GCCTTCCTTC 
CACAGAAGGG TAATTCCACC CAGAGGGAGA ACATCTTTTA AAATCATTTT CCTCCCCACA 
GTGGAGGGCA ATGAAGAAAT CTCTTTATTT ATTAATACAT CAACCCATGG GTTGTTATCA 
TACCAGGTTT TTGGGGTCGG CGTCCATGGG GAATCAGTTA AGGACGCCCA CAGAAAAGAT 
AGCGTTCTGA TATTCCCCCA CATACAGAGC ATTAATCTAA CACAATCTCA GGAAGATGCT 
TCTAATATAA CAATTTTAGG TCTGCTGCTT GACTGCAGTT TACCAAAAAT GTTCTACAAT 
CAGCCCCAGG GTTGGTGTTT AGTATCAGAG GAGGGCAGAA TTGCTCTTCA GATCAGTCTT 
TCTGAAAGAG GGGAAAGGCC GACCCACCTG GACAAACTCA AGCCATACGT GCTGGAGAAT 
ATTGTGGTGC TGCTGGTCCT CCCACCACCA GAAGATGCTG TCTCAGATCC CAAAATAGGA 
GTGTTCATGT TGAACTCAGG TGTTAAAAAG CTCTTTGTTA AGGAGATCCA GCTTTTGTCG 
AGAACAGACA GCAATGTTGA ACTCACTGAA GTCTTGCTCA AGCCTTCTGC TACTAACTTC 
ACTCAAGTGG CTTCACTCTT CTGTAGACAT TCTTTGACGG CTAAAAGTAA AAGGTGTTCA 
AGTCAGTTCG GCCTACACAT TTTAGCAAAC AGAACAGTGA ATTTATATCC ACTGCTGCAA 
AGAACACACA GATGGTCAGA CTCTGAGCTG AGTCCTTTAA TTCAGATCAG ACAGAAGCAG 
AGGGGCTCAG AGCATGTGGA GCTCTGGCTA ACAAACCCTT TCCCCTTCTC CTTCAGAATC 
ACTAACATCT CCCTGTCCCA GCAGAAACTC TTTAAGAGTG TAAATTTTAC AACATCTGTG 
GTAGTGAGCT CGGGGAGCTG GAAGCTTCTC TCTCTTCAGC TACTCGGTAA ATCTTTACCC 
ATCAACCACA GAACCACAAT TATCCTGGCA ACCAGTGTTG GGCTCCCACT AGAGCTTCAC 
CTGCAAACAT ATTCTTCTTC TTCAAAGCAG GGAGTTGTTA TTGTTGAGTC TTCTGAGGAG 
TGTGAACGGC TATGTCCACT CCGTATGTCC AGGGCAGGAC GTGTGGAGTG GCAGCAATCG 
CTTCTCCCAG AATCATCCTC TTCACTATGG AGAGTAGACA GCAGTCTGGC CTCTGCCCTG 
TGCAGTCGTT GGCACTGTAG CAAAGAGCTC TCCTGCAGGT GGCCCAGACT TCCTGAAGAT 
CACTCCACAC CCCTAGACTT TGGTGCCACA CCTGTCAATG ATAGTAAGGT AAAAAATTTC 
ACTCTGAAAA ACCCGACGGG TTCAGTGATT TCTGTGGAGA TACGAACCCT CTCCTCATAC 
CCAGTTCCAC TTGAAGCACT CGACATGCTT ACCAAATGGT TTAATATTAG CCCCCTCTCT 
GTGAATATCA CTACAACAGA GTTTCTGCTG TTGGCTGCTA ACCATAAGGA AAACAAGAGA 
GGTAGTGTGC TGAGAATCCT CCTTCAGCCG TGGGAGTCGA GGACTGTGTC TGTGGCGTAT 
CAACCCACAG AACACAAGCC TGTTACCTCA CTGCTCTTGA TCAGGAACAA CCTGACCGTG 
TTTGACATGG TTCTGGTTAA AGGATTAGGA GCAAAGGAGA TGTTGAGGGT GGGAGGGAAG 
CTTCCTGGAC CTGCAGCCTC TCTGCGCTTC AATGTGCCTC AGTCCACCCT CATGGAGTGC 
AGAGATGGCT TACGATCAAG TAAGCCCTTA TTTGCCATCC AGAAAAGCTT TAAAGTGGAG 
AATGCAGGCG AGTTGCCGCT GACTGTAGTG TCCATGAACA TTAATGGATA TAAATGTCAG 
GGTTATGGTT TTGAAGTGCA GCACTGTGGA TCCTTCAGAG TGGACTACAA CTCCTCCTCA 
GAAATCACTA TTGCATTTAC TCCAGACTTC ACTTCCTCAT GGGTTATTAG AGACCTGACG 
CTGGTGACAG AGCGAGGCTC CTCTTTCCAT TTCACCCTTA ATGTGACTCT TCCTCACCAC 
ATGCTGCCGC TGTGTGCTCA GGTGGTCCCG GGACCCAGCT GGGAGGAGTC TTTCTGGATC 
ATCACACTGG CCTTTACCTG CTTCTCTCTG GCTGTTGTGT GTCTGATGGC TTTTCATCAA 
GCTCAACACA TTCTGAGTGA GTTCTCCATG CCAAATCCAC GTAGCAATCA CAACTCGGTA 
CCTCGAGAAA ATAGCAGTGT TAACCACAGC TCTTCTAATG GTGTCAGTCG AGGAAAAGGC 
AGTTGTAAGA ACTACACTGA CACAAGCCAC CCGTCTGATA AAGGCAAAGG CAGAGGATCT 
CCAGCTGTAG CCAATGGATC AGTTCGGTTG CAGCCTTCAT CCAAGAAGAG CTCTGGAGGT 
TCTTCTCAGC CTCCAAAGAA GCAAACTAAG GTTTCATTAC TATACAGCAG ATACAAAAAC 
AGCCCTGCGA CTCTGGCTGC TAGTGTTTCT GCTACCACTG ACTTCTGTGG TTCGCCCACC 
AATGAAGATA AAGATCACAC CTTTGAATTA GACCCAGAAA TTTGTAATAA CAACAACAAC 
AATAATGATA TATTAGACGA AGCCCTTCTC CCTGTCGAGA AGAAAGAGCA CTTTAGAGAA 
TCCAAGGAAG AGAAGGTCTT GCCAGCTGTT ATGTTTCCCA TGGAAACACT TCTTGGATTA 
CCTGAAAACA TCACAGCAAA CAGAGTCCCT CAGCCTGCAG GCGAAGTGAT GGAAAATGAA 
GAATGTAAAA GAAAAACCAA TGCAGATAAA AGGGATCATC TTGATGAACA GAAAACAGAA 
AGAGGCAGCA GTCAAAAGAA AAGAGCAGAG AAAGATGCAG AACTTGGGGT TTCTGCTCCT 
ATCAACAAAA CGAAGAAAAA CGCAGGCAGG AGTCGCAAGA AGGCACCAGA GCTCATCCCT 
GGGCTTCCTG AAAACAGTGT TGTTATGGTG ACAGAGAGTG AGAGAGAACC ATCACGTGCT 
GCTGCTCGAA CACGAAACGC CAAACCCGAA CTTCCTAAAA CAGGAGCAAA CACAGATAGT 
CCTCTCAAAC ACAGCGGCAC GTGTTTGGGC AGACCTAGGC GTAAGTGTCC AGAGCGGCGT 
CTGCAGTGGG AATCTGGCTC TGACTCCAGC AGTTCATCAG GCAGTGTGCG AGCCAGCCGA 
GGGTCCTGGG GCAGCTGGAG CAGTGCCAGC AGCATAGAGG GGGAAAGAGA TCCAAACAGA 
CACCCCTGCA GGAGAGACAA CATTCAATAC AATCCATATT TAAAAGACCA GGAATGTTAC 
CTGAACATGA ACTATACCTG CAAAAATCCA AGCATGAACA ATCTTTATGA CAGAGAACCA 
TACCAAAGCT CAGAAATGGG TCCTGTCCCA GCACTTCCTC ACACCTTCGC TGATGTAGCT 
GCAGGTGTGG ACAAAAACAT CGACGTAATT GGCTCCAGCA TGATGGAAGA GACGTGGTCA 
GTCAACTCCG TTCCTCTGAC CAATGAATTC AGGTACAACA TGAGTGAATC TCTCCCGTTT 
GTCCCTCAGA ATCCCTCATC CAGATATGAT GGCTTCCCTT GGAACAACAT GAGCAGTCAG 
TGTAGCAGTC AGTATCCCTA CAGTGACCGC AGCAACTACA TGCCAACAGG TAACGGCAAT 
TACCAAAATG CATTCTCATG CCCTGAAAGC CAAACCATAA GCCACAGCCA TCAGCCGGTG 
TGGGGTGAGA ATGGAGCCCA TGATATCAAC TCTACATGGG ACACTGGCAG CTGTGTGGGC 
AGCAAGCCAT ACTTTTCAGG AACCAGAAGT CTGTCCCCCA TGTCCAGTCT ATTTGGTTCA 
ATCTGGACAC CCCAAAGCGA GCCTTACCAG AGTCACTTCC ACCCAGAGCG TTCAGCACCA 
GTCTCGCCTC ACACCCCCCA TACACCCATC TCACCGGTCG CCCCCTTCAG CCGTGAACCC 
GGCGGCGCCT GTCCCGGGAA GCACTTCTCC AGCTTCAACC CCTTCGGACC ACACATGAAT 
TTGGACATAT GGAACTCCTC TTCAAACCGG AGCTCCAACT CTCAGCTCTC CAATGATTCA 
GGCTACTGCG GTGACATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-208H7.4 ( XM_005170953.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005171010.3 Danio rerio si:ch211-208h7.4 (si:ch211-208h7.4), transcript variant X2, mRNA.

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CloneID	ODa53887
Clone ID Related Accession (Same CDS sequence)	XM_005170953.3
Accession Version	XM_005170953.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4638bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	transmembrane protein 131-like isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005170953.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATTGTC GGGCTCTAGT GGGCGCACAC ACTCGCAGAG GTTCTGCCGA AAGAGAGACA 
GACAGAGACA GAGGAGGGAG GGTGAGAGAG AGAGAAAGAG AAAGAAGTGA AGCCAGGGAG 
CTCAACACAG CCAGCATGGC GAGCCAAAGG GACTTCACAC ACAGAAAAAC ATGGATTAAT 
GTTCTCCTGC GACTCTTACA GCTCTTATTG CTTTACATTC AGCACGGAGG AGCTCAGTTT 
CAAGCTCTGA CTCAGATGTC CAGCGTGGTC GAAGTTTGGC AGGCTGAGGA CGCAGACTTC 
TTCGTGCCAT CTCAGGCAGA GGAGCAACAC AGTGAAAATG TTCGAGAGGA GAGCTCCTCG 
TTTTATGTGC AGGAGAGTGG GAGACAGCTC CTGTTTCAGC CGCCCACACT GGATTTTGGA 
CCACAGCCGG TGGGGCTGCC AAGAGCTGAA ACCGTATATA TCCACAATCC CAGCCCAGAG 
CTCCCAGTCT CTCTGCTTTC TGTGTTTACA TCCAGCAGAC ATTTTCACAT GCCTTCCTTC 
CACAGAAGGG TAATTCCACC CAGAGGGAGA ACATCTTTTA AAATCATTTT CCTCCCCACA 
GTGGAGGGCA ATGAAGAAAT CTCTTTATTT ATTAATACAT CAACCCATGG GTTGTTATCA 
TACCAGGTTT TTGGGGTCGG CGTCCATGGG GAATCAGTTA AGGACGCCCA CAGAAAAGAT 
AGCGTTCTGA TATTCCCCCA CATACAGAGC ATTAATCTAA CACAATCTCA GGAAGATGCT 
TCTAATATAA CAATTTTAGG TCTGCTGCTT GACTGCAGTT TACCAAAAAT GTTCTACAAT 
CAGCCCCAGG GTTGGTGTTT AGTATCAGAG GAGGGCAGAA TTGCTCTTCA GATCAGTCTT 
TCTGAAAGAG GGGAAAGGCC GACCCACCTG GACAAACTCA AGCCATACGT GCTGGAGAAT 
ATTGTGGTGC TGCTGGTCCT CCCACCACCA GAAGATGCTG TCTCAGATCC CAAAATAGGA 
GTGTTCATGT TGAACTCAGG TGTTAAAAAG CTCTTTGTTA AGGAGATCCA GCTTTTGTCG 
AGAACAGACA GCAATGTTGA ACTCACTGAA GTCTTGCTCA AGCCTTCTGC TACTAACTTC 
ACTCAAGTGG CTTCACTCTT CTGTAGACAT TCTTTGACGG CTAAAAGTAA AAGGTGTTCA 
AGTCAGTTCG GCCTACACAT TTTAGCAAAC AGAACAGTGA ATTTATATCC ACTGCTGCAA 
AGAACACACA GATGGTCAGA CTCTGAGCTG AGTCCTTTAA TTCAGATCAG ACAGAAGCAG 
AGGGGCTCAG AGCATGTGGA GCTCTGGCTA ACAAACCCTT TCCCCTTCTC CTTCAGAATC 
ACTAACATCT CCCTGTCCCA GCAGAAACTC TTTAAGAGTG TAAATTTTAC AACATCTGTG 
GTAGTGAGCT CGGGGAGCTG GAAGCTTCTC TCTCTTCAGC TACTCGGTAA ATCTTTACCC 
ATCAACCACA GAACCACAAT TATCCTGGCA ACCAGTGTTG GGCTCCCACT AGAGCTTCAC 
CTGCAAACAT ATTCTTCTTC TTCAAAGGGA GTTGTTATTG TTGAGTCTTC TGAGGAGTGT 
GAACGGCTAT GTCCACTCCG TATGTCCAGG GCAGGACGTG TGGAGTGGCA GCAATCGCTT 
CTCCCAGAAT CATCCTCTTC ACTATGGAGA GTAGACAGCA GTCTGGCCTC TGCCCTGTGC 
AGTCGTTGGC ACTGTAGCAA AGAGCTCTCC TGCAGGTGGC CCAGACTTCC TGAAGATCAC 
TCCACACCCC TAGACTTTGG TGCCACACCT GTCAATGATA GTAAGGTAAA AAATTTCACT 
CTGAAAAACC CGACGGGTTC AGTGATTTCT GTGGAGATAC GAACCCTCTC CTCATACCCA 
GTTCCACTTG AAGCACTCGA CATGCTTACC AAATGGTTTA ATATTAGCCC CCTCTCTGTG 
AATATCACTA CAACAGAGTT TCTGCTGTTG GCTGCTAACC ATAAGGAAAA CAAGAGAGGT 
AGTGTGCTGA GAATCCTCCT TCAGCCGTGG GAGTCGAGGA CTGTGTCTGT GGCGTATCAA 
CCCACAGAAC ACAAGCCTGT TACCTCACTG CTCTTGATCA GGAACAACCT GACCGTGTTT 
GACATGGTTC TGGTTAAAGG ATTAGGAGCA AAGGAGATGT TGAGGGTGGG AGGGAAGCTT 
CCTGGACCTG CAGCCTCTCT GCGCTTCAAT GTGCCTCAGT CCACCCTCAT GGAGTGCAGA 
GATGGCTTAC GATCAAGTAA GCCCTTATTT GCCATCCAGA AAAGCTTTAA AGTGGAGAAT 
GCAGGCGAGT TGCCGCTGAC TGTAGTGTCC ATGAACATTA ATGGATATAA ATGTCAGGGT 
TATGGTTTTG AAGTGCAGCA CTGTGGATCC TTCAGAGTGG ACTACAACTC CTCCTCAGAA 
ATCACTATTG CATTTACTCC AGACTTCACT TCCTCATGGG TTATTAGAGA CCTGACGCTG 
GTGACAGAGC GAGGCTCCTC TTTCCATTTC ACCCTTAATG TGACTCTTCC TCACCACATG 
CTGCCGCTGT GTGCTCAGGT GGTCCCGGGA CCCAGCTGGG AGGAGTCTTT CTGGATCATC 
ACACTGGCCT TTACCTGCTT CTCTCTGGCT GTTGTGTGTC TGATGGCTTT TCATCAAGCT 
CAACACATTC TGAGTGAGTT CTCCATGCCA AATCCACGTA GCAATCACAA CTCGGTACCT 
CGAGAAAATA GCAGTGTTAA CCACAGCTCT TCTAATGGTG TCAGTCGAGG AAAAGGCAGT 
TGTAAGAACT ACACTGACAC AAGCCACCCG TCTGATAAAG GCAAAGGCAG AGGATCTCCA 
GCTGTAGCCA ATGGATCAGT TCGGTTGCAG CCTTCATCCA AGAAGAGCTC TGGAGGTTCT 
TCTCAGCCTC CAAAGAAGCA AACTAAGGTT TCATTACTAT ACAGCAGATA CAAAAACAGC 
CCTGCGACTC TGGCTGCTAG TGTTTCTGCT ACCACTGACT TCTGTGGTTC GCCCACCAAT 
GAAGATAAAG ATCACACCTT TGAATTAGAC CCAGAAATTT GTAATAACAA CAACAACAAT 
AATGATATAT TAGACGAAGC CCTTCTCCCT GTCGAGAAGA AAGAGCACTT TAGAGAATCC 
AAGGAAGAGA AGGTCTTGCC AGCTGTTATG TTTCCCATGG AAACACTTCT TGGATTACCT 
GAAAACATCA CAGCAAACAG AGTCCCTCAG CCTGCAGGCG AAGTGATGGA AAATGAAGAA 
TGTAAAAGAA AAACCAATGC AGATAAAAGG GATCATCTTG ATGAACAGAA AACAGAAAGA 
GGCAGCAGTC AAAAGAAAAG AGCAGAGAAA GATGCAGAAC TTGGGGTTTC TGCTCCTATC 
AACAAAACGA AGAAAAACGC AGGCAGGAGT CGCAAGAAGG CACCAGAGCT CATCCCTGGG 
CTTCCTGAAA ACAGTGTTGT TATGGTGACA GAGAGTGAGA GAGAACCATC ACGTGCTGCT 
GCTCGAACAC GAAACGCCAA ACCCGAACTT CCTAAAACAG GAGCAAACAC AGATAGTCCT 
CTCAAACACA GCGGCACGTG TTTGGGCAGA CCTAGGCGTA AGTGTCCAGA GCGGCGTCTG 
CAGTGGGAAT CTGGCTCTGA CTCCAGCAGT TCATCAGGCA GTGTGCGAGC CAGCCGAGGG 
TCCTGGGGCA GCTGGAGCAG TGCCAGCAGC ATAGAGGGGG AAAGAGATCC AAACAGACAC 
CCCTGCAGGA GAGACAACAT TCAATACAAT CCATATTTAA AAGACCAGGA ATGTTACCTG 
AACATGAACT ATACCTGCAA AAATCCAAGC ATGAACAATC TTTATGACAG AGAACCATAC 
CAAAGCTCAG AAATGGGTCC TGTCCCAGCA CTTCCTCACA CCTTCGCTGA TGTAGCTGCA 
GGTGTGGACA AAAACATCGA CGTAATTGGC TCCAGCATGA TGGAAGAGAC GTGGTCAGTC 
AACTCCGTTC CTCTGACCAA TGAATTCAGG TACAACATGA GTGAATCTCT CCCGTTTGTC 
CCTCAGAATC CCTCATCCAG ATATGATGGC TTCCCTTGGA ACAACATGAG CAGTCAGTGT 
AGCAGTCAGT ATCCCTACAG TGACCGCAGC AACTACATGC CAACAGGTAA CGGCAATTAC 
CAAAATGCAT TCTCATGCCC TGAAAGCCAA ACCATAAGCC ACAGCCATCA GCCGGTGTGG 
GGTGAGAATG GAGCCCATGA TATCAACTCT ACATGGGACA CTGGCAGCTG TGTGGGCAGC 
AAGCCATACT TTTCAGGAAC CAGAAGTCTG TCCCCCATGT CCAGTCTATT TGGTTCAATC 
TGGACACCCC AAAGCGAGCC TTACCAGAGT CACTTCCACC CAGAGCGTTC AGCACCAGTC 
TCGCCTCACA CCCCCCATAC ACCCATCTCA CCGGTCGCCC CCTTCAGCCG TGAACCCGGC 
GGCGCCTGTC CCGGGAAGCA CTTCTCCAGC TTCAACCCCT TCGGACCACA CATGAATTTG 
GACATATGGA ACTCCTCTTC AAACCGGAGC TCCAACTCTC AGCTCTCCAA TGATTCAGGC 
TACTGCGGTG ACATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171010.3
CDS	35..4672
Translation	MYCRALVGAHTRRGSAERETDRDRGGRVRERERERSEARELNTASMASQRDFTHRKTWINVLLRLLQLLLLYIQHGGAQFQALTQMSSVVEVWQAEDADFFVPSQAEEQHSENVREESSSFYVQESGRQLLFQPPTLDFGPQPVGLPRAETVYIHNPSPELPVSLLSVFTSSRHFHMPSFHRRVIPPRGRTSFKIIFLPTVEGNEEISLFINTSTHGLLSYQVFGVGVHGESVKDAHRKDSVLIFPHIQSINLTQSQEDASNITILGLLLDCSLPKMFYNQPQGWCLVSEEGRIALQISLSERGERPTHLDKLKPYVLENIVVLLVLPPPEDAVSDPKIGVFMLNSGVKKLFVKEIQLLSRTDSNVELTEVLLKPSATNFTQVASLFCRHSLTAKSKRCSSQFGLHILANRTVNLYPLLQRTHRWSDSELSPLIQIRQKQRGSEHVELWLTNPFPFSFRITNISLSQQKLFKSVNFTTSVVVSSGSWKLLSLQLLGKSLPINHRTTIILATSVGLPLELHLQTYSSSSKGVVIVESSEECERLCPLRMSRAGRVEWQQSLLPESSSSLWRVDSSLASALCSRWHCSKELSCRWPRLPEDHSTPLDFGATPVNDSKVKNFTLKNPTGSVISVEIRTLSSYPVPLEALDMLTKWFNISPLSVNITTTEFLLLAANHKENKRGSVLRILLQPWESRTVSVAYQPTEHKPVTSLLLIRNNLTVFDMVLVKGLGAKEMLRVGGKLPGPAASLRFNVPQSTLMECRDGLRSSKPLFAIQKSFKVENAGELPLTVVSMNINGYKCQGYGFEVQHCGSFRVDYNSSSEITIAFTPDFTSSWVIRDLTLVTERGSSFHFTLNVTLPHHMLPLCAQVVPGPSWEESFWIITLAFTCFSLAVVCLMAFHQAQHILSEFSMPNPRSNHNSVPRENSSVNHSSSNGVSRGKGSCKNYTDTSHPSDKGKGRGSPAVANGSVRLQPSSKKSSGGSSQPPKKQTKVSLLYSRYKNSPATLAASVSATTDFCGSPTNEDKDHTFELDPEICNNNNNNNDILDEALLPVEKKEHFRESKEEKVLPAVMFPMETLLGLPENITANRVPQPAGEVMENEECKRKTNADKRDHLDEQKTERGSSQKKRAEKDAELGVSAPINKTKKNAGRSRKKAPELIPGLPENSVVMVTESEREPSRAAARTRNAKPELPKTGANTDSPLKHSGTCLGRPRRKCPERRLQWESGSDSSSSSGSVRASRGSWGSWSSASSIEGERDPNRHPCRRDNIQYNPYLKDQECYLNMNYTCKNPSMNNLYDREPYQSSEMGPVPALPHTFADVAAGVDKNIDVIGSSMMEETWSVNSVPLTNEFRYNMSESLPFVPQNPSSRYDGFPWNNMSSQCSSQYPYSDRSNYMPTGNGNYQNAFSCPESQTISHSHQPVWGENGAHDINSTWDTGSCVGSKPYFSGTRSLSPMSSLFGSIWTPQSEPYQSHFHPERSAPVSPHTPHTPISPVAPFSREPGGACPGKHFSSFNPFGPHMNLDIWNSSSNRSSNSQLSNDSGYCGDM

Target ORF information:

RefSeq Version	XM_005170953.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-208h7.4 (si:ch211-208h7.4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170953.3

ORF Insert Sequence:

ATGTATTGTC GGGCTCTAGT GGGCGCACAC ACTCGCAGAG GTTCTGCCGA AAGAGAGACA 
GACAGAGACA GAGGAGGGAG GGTGAGAGAG AGAGAAAGAG AAAGAAGTGA AGCCAGGGAG 
CTCAACACAG CCAGCATGGC GAGCCAAAGG GACTTCACAC ACAGAAAAAC ATGGATTAAT 
GTTCTCCTGC GACTCTTACA GCTCTTATTG CTTTACATTC AGCACGGAGG AGCTCAGTTT 
CAAGCTCTGA CTCAGATGTC CAGCGTGGTC GAAGTTTGGC AGGCTGAGGA CGCAGACTTC 
TTCGTGCCAT CTCAGGCAGA GGAGCAACAC AGTGAAAATG TTCGAGAGGA GAGCTCCTCG 
TTTTATGTGC AGGAGAGTGG GAGACAGCTC CTGTTTCAGC CGCCCACACT GGATTTTGGA 
CCACAGCCGG TGGGGCTGCC AAGAGCTGAA ACCGTATATA TCCACAATCC CAGCCCAGAG 
CTCCCAGTCT CTCTGCTTTC TGTGTTTACA TCCAGCAGAC ATTTTCACAT GCCTTCCTTC 
CACAGAAGGG TAATTCCACC CAGAGGGAGA ACATCTTTTA AAATCATTTT CCTCCCCACA 
GTGGAGGGCA ATGAAGAAAT CTCTTTATTT ATTAATACAT CAACCCATGG GTTGTTATCA 
TACCAGGTTT TTGGGGTCGG CGTCCATGGG GAATCAGTTA AGGACGCCCA CAGAAAAGAT 
AGCGTTCTGA TATTCCCCCA CATACAGAGC ATTAATCTAA CACAATCTCA GGAAGATGCT 
TCTAATATAA CAATTTTAGG TCTGCTGCTT GACTGCAGTT TACCAAAAAT GTTCTACAAT 
CAGCCCCAGG GTTGGTGTTT AGTATCAGAG GAGGGCAGAA TTGCTCTTCA GATCAGTCTT 
TCTGAAAGAG GGGAAAGGCC GACCCACCTG GACAAACTCA AGCCATACGT GCTGGAGAAT 
ATTGTGGTGC TGCTGGTCCT CCCACCACCA GAAGATGCTG TCTCAGATCC CAAAATAGGA 
GTGTTCATGT TGAACTCAGG TGTTAAAAAG CTCTTTGTTA AGGAGATCCA GCTTTTGTCG 
AGAACAGACA GCAATGTTGA ACTCACTGAA GTCTTGCTCA AGCCTTCTGC TACTAACTTC 
ACTCAAGTGG CTTCACTCTT CTGTAGACAT TCTTTGACGG CTAAAAGTAA AAGGTGTTCA 
AGTCAGTTCG GCCTACACAT TTTAGCAAAC AGAACAGTGA ATTTATATCC ACTGCTGCAA 
AGAACACACA GATGGTCAGA CTCTGAGCTG AGTCCTTTAA TTCAGATCAG ACAGAAGCAG 
AGGGGCTCAG AGCATGTGGA GCTCTGGCTA ACAAACCCTT TCCCCTTCTC CTTCAGAATC 
ACTAACATCT CCCTGTCCCA GCAGAAACTC TTTAAGAGTG TAAATTTTAC AACATCTGTG 
GTAGTGAGCT CGGGGAGCTG GAAGCTTCTC TCTCTTCAGC TACTCGGTAA ATCTTTACCC 
ATCAACCACA GAACCACAAT TATCCTGGCA ACCAGTGTTG GGCTCCCACT AGAGCTTCAC 
CTGCAAACAT ATTCTTCTTC TTCAAAGGGA GTTGTTATTG TTGAGTCTTC TGAGGAGTGT 
GAACGGCTAT GTCCACTCCG TATGTCCAGG GCAGGACGTG TGGAGTGGCA GCAATCGCTT 
CTCCCAGAAT CATCCTCTTC ACTATGGAGA GTAGACAGCA GTCTGGCCTC TGCCCTGTGC 
AGTCGTTGGC ACTGTAGCAA AGAGCTCTCC TGCAGGTGGC CCAGACTTCC TGAAGATCAC 
TCCACACCCC TAGACTTTGG TGCCACACCT GTCAATGATA GTAAGGTAAA AAATTTCACT 
CTGAAAAACC CGACGGGTTC AGTGATTTCT GTGGAGATAC GAACCCTCTC CTCATACCCA 
GTTCCACTTG AAGCACTCGA CATGCTTACC AAATGGTTTA ATATTAGCCC CCTCTCTGTG 
AATATCACTA CAACAGAGTT TCTGCTGTTG GCTGCTAACC ATAAGGAAAA CAAGAGAGGT 
AGTGTGCTGA GAATCCTCCT TCAGCCGTGG GAGTCGAGGA CTGTGTCTGT GGCGTATCAA 
CCCACAGAAC ACAAGCCTGT TACCTCACTG CTCTTGATCA GGAACAACCT GACCGTGTTT 
GACATGGTTC TGGTTAAAGG ATTAGGAGCA AAGGAGATGT TGAGGGTGGG AGGGAAGCTT 
CCTGGACCTG CAGCCTCTCT GCGCTTCAAT GTGCCTCAGT CCACCCTCAT GGAGTGCAGA 
GATGGCTTAC GATCAAGTAA GCCCTTATTT GCCATCCAGA AAAGCTTTAA AGTGGAGAAT 
GCAGGCGAGT TGCCGCTGAC TGTAGTGTCC ATGAACATTA ATGGATATAA ATGTCAGGGT 
TATGGTTTTG AAGTGCAGCA CTGTGGATCC TTCAGAGTGG ACTACAACTC CTCCTCAGAA 
ATCACTATTG CATTTACTCC AGACTTCACT TCCTCATGGG TTATTAGAGA CCTGACGCTG 
GTGACAGAGC GAGGCTCCTC TTTCCATTTC ACCCTTAATG TGACTCTTCC TCACCACATG 
CTGCCGCTGT GTGCTCAGGT GGTCCCGGGA CCCAGCTGGG AGGAGTCTTT CTGGATCATC 
ACACTGGCCT TTACCTGCTT CTCTCTGGCT GTTGTGTGTC TGATGGCTTT TCATCAAGCT 
CAACACATTC TGAGTGAGTT CTCCATGCCA AATCCACGTA GCAATCACAA CTCGGTACCT 
CGAGAAAATA GCAGTGTTAA CCACAGCTCT TCTAATGGTG TCAGTCGAGG AAAAGGCAGT 
TGTAAGAACT ACACTGACAC AAGCCACCCG TCTGATAAAG GCAAAGGCAG AGGATCTCCA 
GCTGTAGCCA ATGGATCAGT TCGGTTGCAG CCTTCATCCA AGAAGAGCTC TGGAGGTTCT 
TCTCAGCCTC CAAAGAAGCA AACTAAGGTT TCATTACTAT ACAGCAGATA CAAAAACAGC 
CCTGCGACTC TGGCTGCTAG TGTTTCTGCT ACCACTGACT TCTGTGGTTC GCCCACCAAT 
GAAGATAAAG ATCACACCTT TGAATTAGAC CCAGAAATTT GTAATAACAA CAACAACAAT 
AATGATATAT TAGACGAAGC CCTTCTCCCT GTCGAGAAGA AAGAGCACTT TAGAGAATCC 
AAGGAAGAGA AGGTCTTGCC AGCTGTTATG TTTCCCATGG AAACACTTCT TGGATTACCT 
GAAAACATCA CAGCAAACAG AGTCCCTCAG CCTGCAGGCG AAGTGATGGA AAATGAAGAA 
TGTAAAAGAA AAACCAATGC AGATAAAAGG GATCATCTTG ATGAACAGAA AACAGAAAGA 
GGCAGCAGTC AAAAGAAAAG AGCAGAGAAA GATGCAGAAC TTGGGGTTTC TGCTCCTATC 
AACAAAACGA AGAAAAACGC AGGCAGGAGT CGCAAGAAGG CACCAGAGCT CATCCCTGGG 
CTTCCTGAAA ACAGTGTTGT TATGGTGACA GAGAGTGAGA GAGAACCATC ACGTGCTGCT 
GCTCGAACAC GAAACGCCAA ACCCGAACTT CCTAAAACAG GAGCAAACAC AGATAGTCCT 
CTCAAACACA GCGGCACGTG TTTGGGCAGA CCTAGGCGTA AGTGTCCAGA GCGGCGTCTG 
CAGTGGGAAT CTGGCTCTGA CTCCAGCAGT TCATCAGGCA GTGTGCGAGC CAGCCGAGGG 
TCCTGGGGCA GCTGGAGCAG TGCCAGCAGC ATAGAGGGGG AAAGAGATCC AAACAGACAC 
CCCTGCAGGA GAGACAACAT TCAATACAAT CCATATTTAA AAGACCAGGA ATGTTACCTG 
AACATGAACT ATACCTGCAA AAATCCAAGC ATGAACAATC TTTATGACAG AGAACCATAC 
CAAAGCTCAG AAATGGGTCC TGTCCCAGCA CTTCCTCACA CCTTCGCTGA TGTAGCTGCA 
GGTGTGGACA AAAACATCGA CGTAATTGGC TCCAGCATGA TGGAAGAGAC GTGGTCAGTC 
AACTCCGTTC CTCTGACCAA TGAATTCAGG TACAACATGA GTGAATCTCT CCCGTTTGTC 
CCTCAGAATC CCTCATCCAG ATATGATGGC TTCCCTTGGA ACAACATGAG CAGTCAGTGT 
AGCAGTCAGT ATCCCTACAG TGACCGCAGC AACTACATGC CAACAGGTAA CGGCAATTAC 
CAAAATGCAT TCTCATGCCC TGAAAGCCAA ACCATAAGCC ACAGCCATCA GCCGGTGTGG 
GGTGAGAATG GAGCCCATGA TATCAACTCT ACATGGGACA CTGGCAGCTG TGTGGGCAGC 
AAGCCATACT TTTCAGGAAC CAGAAGTCTG TCCCCCATGT CCAGTCTATT TGGTTCAATC 
TGGACACCCC AAAGCGAGCC TTACCAGAGT CACTTCCACC CAGAGCGTTC AGCACCAGTC 
TCGCCTCACA CCCCCCATAC ACCCATCTCA CCGGTCGCCC CCTTCAGCCG TGAACCCGGC 
GGCGCCTGTC CCGGGAAGCA CTTCTCCAGC TTCAACCCCT TCGGACCACA CATGAATTTG 
GACATATGGA ACTCCTCTTC AAACCGGAGC TCCAACTCTC AGCTCTCCAA TGATTCAGGC 
TACTGCGGTG ACATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-208H7.4 ( XM_009291318.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009289593.2 Danio rerio si:ch211-208h7.4 (si:ch211-208h7.4), transcript variant X3, mRNA.

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CloneID	ODa53888
Clone ID Related Accession (Same CDS sequence)	XM_009291318.2
Accession Version	XM_009291318.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4638bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	transmembrane protein 131-like isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291318.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATTGTC GGGCTCTAGT GGGCGCACAC ACTCGCAGAG GTTCTGCCGA AAGAGAGACA 
GACAGAGACA GAGGAGGGAG GGTGAGAGAG AGAGAAAGAG AAAGAAGTGA AGCCAGGGAG 
CTCAACACAG CCAGCATGGC GAGCCAAAGG GACTTCACAC ACAGAAAAAC ATGGATTAAT 
GTTCTCCTGC GACTCTTACA GCTCTTATTG CTTTACATTC AGCACGGAGG AGCTCAGTTT 
CAAGCTCTGA CTCAGATGTC CAGCGTGGTC GAAGTTTGGC AGGCTGAGGA CGCAGACTTC 
TTCGTGCCAT CTCAGGCAGA GGAGCAACAC AGTGAAAATG TTCGAGAGGA GAGCTCCTCG 
TTTTATGTGC AGGAGAGTGG GAGACAGCTC CTGTTTCAGC CGCCCACACT GGATTTTGGA 
CCACAGCCGG TGGGGCTGCC AAGAGCTGAA ACCGTATATA TCCACAATCC CAGCCCAGAG 
CTCCCAGTCT CTCTGCTTTC TGTGTTTACA TCCAGCAGAC ATTTTCACAT GCCTTCCTTC 
CACAGAAGGG TAATTCCACC CAGAGGGAGA ACATCTTTTA AAATCATTTT CCTCCCCACA 
GTGGAGGGCA ATGAAGAAAT CTCTTTATTT ATTAATACAT CAACCCATGG GTTGTTATCA 
TACCAGGTTT TTGGGGTCGG CGTCCATGGG GAATCAGTTA AGGACGCCCA CAGAAAAGAT 
AGCGTTCTGA TATTCCCCCA CATACAGAGC ATTAATCTAA CACAATCTCA GGAAGATGCT 
TCTAATATAA CAATTTTAGG TCTGCTGCTT GACTGCAGTT TACCAAAAAT GTTCTACAAT 
CAGCCCCAGG GTTGGTGTTT AGTATCAGAG GAGGGCAGAA TTGCTCTTCA GATCAGTCTT 
TCTGAAAGAG GGGAAAGGCC GACCCACCTG GACAAACTCA AGCCATACGT GCTGGAGAAT 
ATTGTGGTGC TGCTGGTCCT CCCACCACCA GAAGATGCTG TCTATCCCAA AATAGGAGTG 
TTCATGTTGA ACTCAGGTGT TAAAAAGCTC TTTGTTAAGG AGATCCAGCT TTTGTCGAGA 
ACAGACAGCA ATGTTGAACT CACTGAAGTC TTGCTCAAGC CTTCTGCTAC TAACTTCACT 
CAAGTGGCTT CACTCTTCTG TAGACATTCT TTGACGGCTA AAAGTAAAAG GTGTTCAAGT 
CAGTTCGGCC TACACATTTT AGCAAACAGA ACAGTGAATT TATATCCACT GCTGCAAAGA 
ACACACAGAT GGTCAGACTC TGAGCTGAGT CCTTTAATTC AGATCAGACA GAAGCAGAGG 
GGCTCAGAGC ATGTGGAGCT CTGGCTAACA AACCCTTTCC CCTTCTCCTT CAGAATCACT 
AACATCTCCC TGTCCCAGCA GAAACTCTTT AAGAGTGTAA ATTTTACAAC ATCTGTGGTA 
GTGAGCTCGG GGAGCTGGAA GCTTCTCTCT CTTCAGCTAC TCGGTAAATC TTTACCCATC 
AACCACAGAA CCACAATTAT CCTGGCAACC AGTGTTGGGC TCCCACTAGA GCTTCACCTG 
CAAACATATT CTTCTTCTTC AAAGCAGGGA GTTGTTATTG TTGAGTCTTC TGAGGAGTGT 
GAACGGCTAT GTCCACTCCG TATGTCCAGG GCAGGACGTG TGGAGTGGCA GCAATCGCTT 
CTCCCAGAAT CATCCTCTTC ACTATGGAGA GTAGACAGCA GTCTGGCCTC TGCCCTGTGC 
AGTCGTTGGC ACTGTAGCAA AGAGCTCTCC TGCAGGTGGC CCAGACTTCC TGAAGATCAC 
TCCACACCCC TAGACTTTGG TGCCACACCT GTCAATGATA GTAAGGTAAA AAATTTCACT 
CTGAAAAACC CGACGGGTTC AGTGATTTCT GTGGAGATAC GAACCCTCTC CTCATACCCA 
GTTCCACTTG AAGCACTCGA CATGCTTACC AAATGGTTTA ATATTAGCCC CCTCTCTGTG 
AATATCACTA CAACAGAGTT TCTGCTGTTG GCTGCTAACC ATAAGGAAAA CAAGAGAGGT 
AGTGTGCTGA GAATCCTCCT TCAGCCGTGG GAGTCGAGGA CTGTGTCTGT GGCGTATCAA 
CCCACAGAAC ACAAGCCTGT TACCTCACTG CTCTTGATCA GGAACAACCT GACCGTGTTT 
GACATGGTTC TGGTTAAAGG ATTAGGAGCA AAGGAGATGT TGAGGGTGGG AGGGAAGCTT 
CCTGGACCTG CAGCCTCTCT GCGCTTCAAT GTGCCTCAGT CCACCCTCAT GGAGTGCAGA 
GATGGCTTAC GATCAAGTAA GCCCTTATTT GCCATCCAGA AAAGCTTTAA AGTGGAGAAT 
GCAGGCGAGT TGCCGCTGAC TGTAGTGTCC ATGAACATTA ATGGATATAA ATGTCAGGGT 
TATGGTTTTG AAGTGCAGCA CTGTGGATCC TTCAGAGTGG ACTACAACTC CTCCTCAGAA 
ATCACTATTG CATTTACTCC AGACTTCACT TCCTCATGGG TTATTAGAGA CCTGACGCTG 
GTGACAGAGC GAGGCTCCTC TTTCCATTTC ACCCTTAATG TGACTCTTCC TCACCACATG 
CTGCCGCTGT GTGCTCAGGT GGTCCCGGGA CCCAGCTGGG AGGAGTCTTT CTGGATCATC 
ACACTGGCCT TTACCTGCTT CTCTCTGGCT GTTGTGTGTC TGATGGCTTT TCATCAAGCT 
CAACACATTC TGAGTGAGTT CTCCATGCCA AATCCACGTA GCAATCACAA CTCGGTACCT 
CGAGAAAATA GCAGTGTTAA CCACAGCTCT TCTAATGGTG TCAGTCGAGG AAAAGGCAGT 
TGTAAGAACT ACACTGACAC AAGCCACCCG TCTGATAAAG GCAAAGGCAG AGGATCTCCA 
GCTGTAGCCA ATGGATCAGT TCGGTTGCAG CCTTCATCCA AGAAGAGCTC TGGAGGTTCT 
TCTCAGCCTC CAAAGAAGCA AACTAAGGTT TCATTACTAT ACAGCAGATA CAAAAACAGC 
CCTGCGACTC TGGCTGCTAG TGTTTCTGCT ACCACTGACT TCTGTGGTTC GCCCACCAAT 
GAAGATAAAG ATCACACCTT TGAATTAGAC CCAGAAATTT GTAATAACAA CAACAACAAT 
AATGATATAT TAGACGAAGC CCTTCTCCCT GTCGAGAAGA AAGAGCACTT TAGAGAATCC 
AAGGAAGAGA AGGTCTTGCC AGCTGTTATG TTTCCCATGG AAACACTTCT TGGATTACCT 
GAAAACATCA CAGCAAACAG AGTCCCTCAG CCTGCAGGCG AAGTGATGGA AAATGAAGAA 
TGTAAAAGAA AAACCAATGC AGATAAAAGG GATCATCTTG ATGAACAGAA AACAGAAAGA 
GGCAGCAGTC AAAAGAAAAG AGCAGAGAAA GATGCAGAAC TTGGGGTTTC TGCTCCTATC 
AACAAAACGA AGAAAAACGC AGGCAGGAGT CGCAAGAAGG CACCAGAGCT CATCCCTGGG 
CTTCCTGAAA ACAGTGTTGT TATGGTGACA GAGAGTGAGA GAGAACCATC ACGTGCTGCT 
GCTCGAACAC GAAACGCCAA ACCCGAACTT CCTAAAACAG GAGCAAACAC AGATAGTCCT 
CTCAAACACA GCGGCACGTG TTTGGGCAGA CCTAGGCGTA AGTGTCCAGA GCGGCGTCTG 
CAGTGGGAAT CTGGCTCTGA CTCCAGCAGT TCATCAGGCA GTGTGCGAGC CAGCCGAGGG 
TCCTGGGGCA GCTGGAGCAG TGCCAGCAGC ATAGAGGGGG AAAGAGATCC AAACAGACAC 
CCCTGCAGGA GAGACAACAT TCAATACAAT CCATATTTAA AAGACCAGGA ATGTTACCTG 
AACATGAACT ATACCTGCAA AAATCCAAGC ATGAACAATC TTTATGACAG AGAACCATAC 
CAAAGCTCAG AAATGGGTCC TGTCCCAGCA CTTCCTCACA CCTTCGCTGA TGTAGCTGCA 
GGTGTGGACA AAAACATCGA CGTAATTGGC TCCAGCATGA TGGAAGAGAC GTGGTCAGTC 
AACTCCGTTC CTCTGACCAA TGAATTCAGG TACAACATGA GTGAATCTCT CCCGTTTGTC 
CCTCAGAATC CCTCATCCAG ATATGATGGC TTCCCTTGGA ACAACATGAG CAGTCAGTGT 
AGCAGTCAGT ATCCCTACAG TGACCGCAGC AACTACATGC CAACAGGTAA CGGCAATTAC 
CAAAATGCAT TCTCATGCCC TGAAAGCCAA ACCATAAGCC ACAGCCATCA GCCGGTGTGG 
GGTGAGAATG GAGCCCATGA TATCAACTCT ACATGGGACA CTGGCAGCTG TGTGGGCAGC 
AAGCCATACT TTTCAGGAAC CAGAAGTCTG TCCCCCATGT CCAGTCTATT TGGTTCAATC 
TGGACACCCC AAAGCGAGCC TTACCAGAGT CACTTCCACC CAGAGCGTTC AGCACCAGTC 
TCGCCTCACA CCCCCCATAC ACCCATCTCA CCGGTCGCCC CCTTCAGCCG TGAACCCGGC 
GGCGCCTGTC CCGGGAAGCA CTTCTCCAGC TTCAACCCCT TCGGACCACA CATGAATTTG 
GACATATGGA ACTCCTCTTC AAACCGGAGC TCCAACTCTC AGCTCTCCAA TGATTCAGGC 
TACTGCGGTG ACATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289593.2
CDS	35..4672
Translation	MYCRALVGAHTRRGSAERETDRDRGGRVRERERERSEARELNTASMASQRDFTHRKTWINVLLRLLQLLLLYIQHGGAQFQALTQMSSVVEVWQAEDADFFVPSQAEEQHSENVREESSSFYVQESGRQLLFQPPTLDFGPQPVGLPRAETVYIHNPSPELPVSLLSVFTSSRHFHMPSFHRRVIPPRGRTSFKIIFLPTVEGNEEISLFINTSTHGLLSYQVFGVGVHGESVKDAHRKDSVLIFPHIQSINLTQSQEDASNITILGLLLDCSLPKMFYNQPQGWCLVSEEGRIALQISLSERGERPTHLDKLKPYVLENIVVLLVLPPPEDAVYPKIGVFMLNSGVKKLFVKEIQLLSRTDSNVELTEVLLKPSATNFTQVASLFCRHSLTAKSKRCSSQFGLHILANRTVNLYPLLQRTHRWSDSELSPLIQIRQKQRGSEHVELWLTNPFPFSFRITNISLSQQKLFKSVNFTTSVVVSSGSWKLLSLQLLGKSLPINHRTTIILATSVGLPLELHLQTYSSSSKQGVVIVESSEECERLCPLRMSRAGRVEWQQSLLPESSSSLWRVDSSLASALCSRWHCSKELSCRWPRLPEDHSTPLDFGATPVNDSKVKNFTLKNPTGSVISVEIRTLSSYPVPLEALDMLTKWFNISPLSVNITTTEFLLLAANHKENKRGSVLRILLQPWESRTVSVAYQPTEHKPVTSLLLIRNNLTVFDMVLVKGLGAKEMLRVGGKLPGPAASLRFNVPQSTLMECRDGLRSSKPLFAIQKSFKVENAGELPLTVVSMNINGYKCQGYGFEVQHCGSFRVDYNSSSEITIAFTPDFTSSWVIRDLTLVTERGSSFHFTLNVTLPHHMLPLCAQVVPGPSWEESFWIITLAFTCFSLAVVCLMAFHQAQHILSEFSMPNPRSNHNSVPRENSSVNHSSSNGVSRGKGSCKNYTDTSHPSDKGKGRGSPAVANGSVRLQPSSKKSSGGSSQPPKKQTKVSLLYSRYKNSPATLAASVSATTDFCGSPTNEDKDHTFELDPEICNNNNNNNDILDEALLPVEKKEHFRESKEEKVLPAVMFPMETLLGLPENITANRVPQPAGEVMENEECKRKTNADKRDHLDEQKTERGSSQKKRAEKDAELGVSAPINKTKKNAGRSRKKAPELIPGLPENSVVMVTESEREPSRAAARTRNAKPELPKTGANTDSPLKHSGTCLGRPRRKCPERRLQWESGSDSSSSSGSVRASRGSWGSWSSASSIEGERDPNRHPCRRDNIQYNPYLKDQECYLNMNYTCKNPSMNNLYDREPYQSSEMGPVPALPHTFADVAAGVDKNIDVIGSSMMEETWSVNSVPLTNEFRYNMSESLPFVPQNPSSRYDGFPWNNMSSQCSSQYPYSDRSNYMPTGNGNYQNAFSCPESQTISHSHQPVWGENGAHDINSTWDTGSCVGSKPYFSGTRSLSPMSSLFGSIWTPQSEPYQSHFHPERSAPVSPHTPHTPISPVAPFSREPGGACPGKHFSSFNPFGPHMNLDIWNSSSNRSSNSQLSNDSGYCGDM

Target ORF information:

RefSeq Version	XM_009291318.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-208h7.4 (si:ch211-208h7.4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291318.2

ORF Insert Sequence:

ATGTATTGTC GGGCTCTAGT GGGCGCACAC ACTCGCAGAG GTTCTGCCGA AAGAGAGACA 
GACAGAGACA GAGGAGGGAG GGTGAGAGAG AGAGAAAGAG AAAGAAGTGA AGCCAGGGAG 
CTCAACACAG CCAGCATGGC GAGCCAAAGG GACTTCACAC ACAGAAAAAC ATGGATTAAT 
GTTCTCCTGC GACTCTTACA GCTCTTATTG CTTTACATTC AGCACGGAGG AGCTCAGTTT 
CAAGCTCTGA CTCAGATGTC CAGCGTGGTC GAAGTTTGGC AGGCTGAGGA CGCAGACTTC 
TTCGTGCCAT CTCAGGCAGA GGAGCAACAC AGTGAAAATG TTCGAGAGGA GAGCTCCTCG 
TTTTATGTGC AGGAGAGTGG GAGACAGCTC CTGTTTCAGC CGCCCACACT GGATTTTGGA 
CCACAGCCGG TGGGGCTGCC AAGAGCTGAA ACCGTATATA TCCACAATCC CAGCCCAGAG 
CTCCCAGTCT CTCTGCTTTC TGTGTTTACA TCCAGCAGAC ATTTTCACAT GCCTTCCTTC 
CACAGAAGGG TAATTCCACC CAGAGGGAGA ACATCTTTTA AAATCATTTT CCTCCCCACA 
GTGGAGGGCA ATGAAGAAAT CTCTTTATTT ATTAATACAT CAACCCATGG GTTGTTATCA 
TACCAGGTTT TTGGGGTCGG CGTCCATGGG GAATCAGTTA AGGACGCCCA CAGAAAAGAT 
AGCGTTCTGA TATTCCCCCA CATACAGAGC ATTAATCTAA CACAATCTCA GGAAGATGCT 
TCTAATATAA CAATTTTAGG TCTGCTGCTT GACTGCAGTT TACCAAAAAT GTTCTACAAT 
CAGCCCCAGG GTTGGTGTTT AGTATCAGAG GAGGGCAGAA TTGCTCTTCA GATCAGTCTT 
TCTGAAAGAG GGGAAAGGCC GACCCACCTG GACAAACTCA AGCCATACGT GCTGGAGAAT 
ATTGTGGTGC TGCTGGTCCT CCCACCACCA GAAGATGCTG TCTATCCCAA AATAGGAGTG 
TTCATGTTGA ACTCAGGTGT TAAAAAGCTC TTTGTTAAGG AGATCCAGCT TTTGTCGAGA 
ACAGACAGCA ATGTTGAACT CACTGAAGTC TTGCTCAAGC CTTCTGCTAC TAACTTCACT 
CAAGTGGCTT CACTCTTCTG TAGACATTCT TTGACGGCTA AAAGTAAAAG GTGTTCAAGT 
CAGTTCGGCC TACACATTTT AGCAAACAGA ACAGTGAATT TATATCCACT GCTGCAAAGA 
ACACACAGAT GGTCAGACTC TGAGCTGAGT CCTTTAATTC AGATCAGACA GAAGCAGAGG 
GGCTCAGAGC ATGTGGAGCT CTGGCTAACA AACCCTTTCC CCTTCTCCTT CAGAATCACT 
AACATCTCCC TGTCCCAGCA GAAACTCTTT AAGAGTGTAA ATTTTACAAC ATCTGTGGTA 
GTGAGCTCGG GGAGCTGGAA GCTTCTCTCT CTTCAGCTAC TCGGTAAATC TTTACCCATC 
AACCACAGAA CCACAATTAT CCTGGCAACC AGTGTTGGGC TCCCACTAGA GCTTCACCTG 
CAAACATATT CTTCTTCTTC AAAGCAGGGA GTTGTTATTG TTGAGTCTTC TGAGGAGTGT 
GAACGGCTAT GTCCACTCCG TATGTCCAGG GCAGGACGTG TGGAGTGGCA GCAATCGCTT 
CTCCCAGAAT CATCCTCTTC ACTATGGAGA GTAGACAGCA GTCTGGCCTC TGCCCTGTGC 
AGTCGTTGGC ACTGTAGCAA AGAGCTCTCC TGCAGGTGGC CCAGACTTCC TGAAGATCAC 
TCCACACCCC TAGACTTTGG TGCCACACCT GTCAATGATA GTAAGGTAAA AAATTTCACT 
CTGAAAAACC CGACGGGTTC AGTGATTTCT GTGGAGATAC GAACCCTCTC CTCATACCCA 
GTTCCACTTG AAGCACTCGA CATGCTTACC AAATGGTTTA ATATTAGCCC CCTCTCTGTG 
AATATCACTA CAACAGAGTT TCTGCTGTTG GCTGCTAACC ATAAGGAAAA CAAGAGAGGT 
AGTGTGCTGA GAATCCTCCT TCAGCCGTGG GAGTCGAGGA CTGTGTCTGT GGCGTATCAA 
CCCACAGAAC ACAAGCCTGT TACCTCACTG CTCTTGATCA GGAACAACCT GACCGTGTTT 
GACATGGTTC TGGTTAAAGG ATTAGGAGCA AAGGAGATGT TGAGGGTGGG AGGGAAGCTT 
CCTGGACCTG CAGCCTCTCT GCGCTTCAAT GTGCCTCAGT CCACCCTCAT GGAGTGCAGA 
GATGGCTTAC GATCAAGTAA GCCCTTATTT GCCATCCAGA AAAGCTTTAA AGTGGAGAAT 
GCAGGCGAGT TGCCGCTGAC TGTAGTGTCC ATGAACATTA ATGGATATAA ATGTCAGGGT 
TATGGTTTTG AAGTGCAGCA CTGTGGATCC TTCAGAGTGG ACTACAACTC CTCCTCAGAA 
ATCACTATTG CATTTACTCC AGACTTCACT TCCTCATGGG TTATTAGAGA CCTGACGCTG 
GTGACAGAGC GAGGCTCCTC TTTCCATTTC ACCCTTAATG TGACTCTTCC TCACCACATG 
CTGCCGCTGT GTGCTCAGGT GGTCCCGGGA CCCAGCTGGG AGGAGTCTTT CTGGATCATC 
ACACTGGCCT TTACCTGCTT CTCTCTGGCT GTTGTGTGTC TGATGGCTTT TCATCAAGCT 
CAACACATTC TGAGTGAGTT CTCCATGCCA AATCCACGTA GCAATCACAA CTCGGTACCT 
CGAGAAAATA GCAGTGTTAA CCACAGCTCT TCTAATGGTG TCAGTCGAGG AAAAGGCAGT 
TGTAAGAACT ACACTGACAC AAGCCACCCG TCTGATAAAG GCAAAGGCAG AGGATCTCCA 
GCTGTAGCCA ATGGATCAGT TCGGTTGCAG CCTTCATCCA AGAAGAGCTC TGGAGGTTCT 
TCTCAGCCTC CAAAGAAGCA AACTAAGGTT TCATTACTAT ACAGCAGATA CAAAAACAGC 
CCTGCGACTC TGGCTGCTAG TGTTTCTGCT ACCACTGACT TCTGTGGTTC GCCCACCAAT 
GAAGATAAAG ATCACACCTT TGAATTAGAC CCAGAAATTT GTAATAACAA CAACAACAAT 
AATGATATAT TAGACGAAGC CCTTCTCCCT GTCGAGAAGA AAGAGCACTT TAGAGAATCC 
AAGGAAGAGA AGGTCTTGCC AGCTGTTATG TTTCCCATGG AAACACTTCT TGGATTACCT 
GAAAACATCA CAGCAAACAG AGTCCCTCAG CCTGCAGGCG AAGTGATGGA AAATGAAGAA 
TGTAAAAGAA AAACCAATGC AGATAAAAGG GATCATCTTG ATGAACAGAA AACAGAAAGA 
GGCAGCAGTC AAAAGAAAAG AGCAGAGAAA GATGCAGAAC TTGGGGTTTC TGCTCCTATC 
AACAAAACGA AGAAAAACGC AGGCAGGAGT CGCAAGAAGG CACCAGAGCT CATCCCTGGG 
CTTCCTGAAA ACAGTGTTGT TATGGTGACA GAGAGTGAGA GAGAACCATC ACGTGCTGCT 
GCTCGAACAC GAAACGCCAA ACCCGAACTT CCTAAAACAG GAGCAAACAC AGATAGTCCT 
CTCAAACACA GCGGCACGTG TTTGGGCAGA CCTAGGCGTA AGTGTCCAGA GCGGCGTCTG 
CAGTGGGAAT CTGGCTCTGA CTCCAGCAGT TCATCAGGCA GTGTGCGAGC CAGCCGAGGG 
TCCTGGGGCA GCTGGAGCAG TGCCAGCAGC ATAGAGGGGG AAAGAGATCC AAACAGACAC 
CCCTGCAGGA GAGACAACAT TCAATACAAT CCATATTTAA AAGACCAGGA ATGTTACCTG 
AACATGAACT ATACCTGCAA AAATCCAAGC ATGAACAATC TTTATGACAG AGAACCATAC 
CAAAGCTCAG AAATGGGTCC TGTCCCAGCA CTTCCTCACA CCTTCGCTGA TGTAGCTGCA 
GGTGTGGACA AAAACATCGA CGTAATTGGC TCCAGCATGA TGGAAGAGAC GTGGTCAGTC 
AACTCCGTTC CTCTGACCAA TGAATTCAGG TACAACATGA GTGAATCTCT CCCGTTTGTC 
CCTCAGAATC CCTCATCCAG ATATGATGGC TTCCCTTGGA ACAACATGAG CAGTCAGTGT 
AGCAGTCAGT ATCCCTACAG TGACCGCAGC AACTACATGC CAACAGGTAA CGGCAATTAC 
CAAAATGCAT TCTCATGCCC TGAAAGCCAA ACCATAAGCC ACAGCCATCA GCCGGTGTGG 
GGTGAGAATG GAGCCCATGA TATCAACTCT ACATGGGACA CTGGCAGCTG TGTGGGCAGC 
AAGCCATACT TTTCAGGAAC CAGAAGTCTG TCCCCCATGT CCAGTCTATT TGGTTCAATC 
TGGACACCCC AAAGCGAGCC TTACCAGAGT CACTTCCACC CAGAGCGTTC AGCACCAGTC 
TCGCCTCACA CCCCCCATAC ACCCATCTCA CCGGTCGCCC CCTTCAGCCG TGAACCCGGC 
GGCGCCTGTC CCGGGAAGCA CTTCTCCAGC TTCAACCCCT TCGGACCACA CATGAATTTG 
GACATATGGA ACTCCTCTTC AAACCGGAGC TCCAACTCTC AGCTCTCCAA TGATTCAGGC 
TACTGCGGTG ACATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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SI:CH211-208H7.4 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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