ash1l cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ash1l
Entrez Gene ID	563799
Full Name	ash1 (absent, small, or homeotic)-like (Drosophila)
Synonyms	fi34d11,si:ch211-81a5.3,wu:fi34d11
General protein information	Preferred Names ash1 (absent, small, or homeotic)-like (Drosophila) Names probable histone-lysine N-methyltransferase ASH1L
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001346407.1	NP_001333336.1	probable histone-lysine N-methyltransferase ASH1L
XM_005159483.3	XP_005159540.1	histone-lysine N-methyltransferase ASH1L isoform X1
XM_005159484.3	XP_005159541.1	histone-lysine N-methyltransferase ASH1L isoform X1
XM_005159484.4	XP_005159541.1	probable histone-lysine N-methyltransferase ASH1L isoform X1
XM_005159483.4	XP_005159540.1	probable histone-lysine N-methyltransferase ASH1L isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Huang HT, Kathrein KL, Barton A, Gitlin Z, Huang YH, Ward TP, Hofmann O, Dibiase A, Song A, Tyekucheva S, Hide W, Zhou Y, Zon LI
Nature cell biology15(12)1516-25(2013 Dec)

Genome-wide survey and developmental expression mapping of zebrafish SET domain-containing genes.
Sun XJ, Xu PF, Zhou T, Hu M, Fu CT, Zhang Y, Jin Y, Chen Y, Chen SJ, Huang QH, Liu TX, Chen Z
PloS one3(1)e1499(2008 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ash1l gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ash1l cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa23936	NM_001346407.1 Latest version!	Danio rerio ash1 (absent, small, or homeotic)-like (Drosophila) (ash1l), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa23935	XM_005159484.4 Latest version!	Danio rerio ash1 (absent, small, or homeotic)-like (Drosophila) (ash1l), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa23935	XM_005159483.4 Latest version!	Danio rerio ash1 (absent, small, or homeotic)-like (Drosophila) (ash1l), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
ODa23935	XM_005159484.3	Danio rerio ash1 (absent, small, or homeotic)-like (Drosophila) (ash1l), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa23935	XM_005159483.3	Danio rerio ash1 (absent, small, or homeotic)-like (Drosophila) (ash1l), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ash1l ( NM_001346407.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001333336.1 Danio rerio ash1 (absent, small, or homeotic)-like (Drosophila) (ash1l), mRNA.

Price & Availability↑

CloneID	ODa23936
Clone ID Related Accession (Same CDS sequence)	NM_001346407.1
Accession Version	NM_001346407.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	probable histone-lysine N-methyltransferase ASH1L
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR626935.6. On Oct 9, 2016 this sequence version replaced XM_687162.7. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01001675.1, GDQH01005255.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGATCAGA GGACACAGGG GGGGCCTTCT ACGCCGCCCC CACTCCCTGC CAATGCCCCC 
CCAGCAGAAC GTGAAAAAGA AGGTGTAGGA GGAAAGAAGG ACGATGAAGA AGAGAAAAAG 
AAACGGGAGA AACTAAGAGA TGGGGCACAG AATGAAGCTA GTGGTGCTGA AAGTGGGACT 
GGTGACCAGC CGCAACAACC CACGCAGTTT TCGGTCAAGG AGACCAGCTA CTCTGAGGGT 
AACGTTAAGC TCAAAATAGG CCTGCAGGCC AAACGCATGA AGAAACCTCC TAAGATCCTG 
GAGAACTATG TCTGCCGACC AGCCTTCCGT GCCACTGTAA GGCATAGTGG AGGTAGAGGC 
GGAGGACGTG CTAATCGTGG GGCTGGAGCC AGTGATGCGA ACAGCAACTC TACTAGCACA 
AGTAATGGAA AGGAAGGGGA GAAAAAGCCC TCTGTCAACC GAGGAGCATC TCAGTCAGCT 
GCTGCTCCTG CTGCTTCTAC TCCCTCACCC TCTCTTCCTC CTGCTTCCAT CAACACTGTC 
ACTCCAGTGA ATGGAAATGC TCCAGCAAAA AGGGGTCCTC CAAAACTTGC CTGCAAATCA 
GAGGGAAAGG CTGACGTAAA ATCTGTTGCT TCATCAGAGA AACCTCTTAA TCTCCATCGC 
CCCACGACAG AAAGTAAATC ACACTCCTCT GGAAAGAAAA ACCATGTTCC ACAAACAAAC 
TCTGCCCCTG CCCCATCTCC ACCAGCAGTG CCCCCAGCTG CCACTTCGCA ACAGGACCCT 
AAAATAGATG GAATCTTAAA AACGCCTCAA TGCAATTTTT TGGAGGGGCA GAAGGAACAA 
GAAAGAGGGA TTTCTTCTTG GGGTACCCCT ACAGTAACTG AAAAACTTGC TCAGCTCATA 
GCTACATGCC CACCTTCAAA GTGCCCTAAG CCAAAAGCCC GAAAGGTATC GCCAGCTCCA 
GCACACAGTA GCTCACCTCC GTTAATTTCT AATCTGCAGC GCCCTGAACG GGCAATGGCT 
AATAGAAACA CCTATTCCAG GGCGGTTCAC CTCGCCCAAC CACCACCTGT ATCACGGCCC 
CCTGGTCGCC CATATGGGTC TAAAAACAAA GACAGTATCC TTGAGAAATT TTCTGGAACT 
TCTAAAAAAG AGGAATCAGA TGGACTTTCA AAAGGGGTCT CCGGTAGCAG CAGCACAACC 
ACCACCACCA CTAGTTCAGC ACTGTCTCAA AACAGCAGTC GCTTGACGAC AACAACATCC 
ACAAATGAAA GCACTGACAT TAACAACACA GATGGAATGA AATCTCAGTC TCGTCTTGGG 
CATTGCCCAT CTCATTCTTC ATCCGCAGCT GCTTCACCTC CATTTTCTTC TTCATCCTCC 
GTAGAGAGAA TAGGAAGTAC AAGCTGTAAC ATAGGTTTGC CAGGCTCTCT AACCTCACGC 
CCTAAAGCAA GCCCCTCTCG AATAGAGGAT TTTAGTGAGA AAGACAAAGA ACGTGACCGG 
GAAAAGGACA CAGACAGTCC AAGAGATTTA AGTAAAGGTA TCACTCCAGC TATAACTGGG 
AAGCAGGATG CAAAAGAAAG GTGTTCTTTA TCAGCTCCTC GAAATGACAG GGGTAAGAGC 
TCAAGCCCAG TCAAACGTAG TCCCAATCAG GAAAGTCGCG TTAGTGGGGT ACCAAGTCCT 
GCTGAGTCCA CCAAATGCAC TATTTCTCCT TCATACCCTT CTGGTGCCAG CAGGAGCCCA 
CCACCACTAT GCGATGACAC CGGGGCTATT TCTCCTGCAT CACCATCTGA GCAGGATTCA 
AAACCTCTTA AGAAGCGACG AGGACGACGA CCTCGCTGGA CGAAGGCAGT GAGTCGCACC 
CACAAGTCTT TACATGAGTC TCCTTCTAAC CATCAGCAAA TCCCCACACC AATGCCCTCA 
GGGCTTTCTT TCTCATCCCC TATTCGGGAG TCTGTGGAGC AGCCTCTCAG TGACAGTTAC 
ACATCCCCAG AGGATTCTGA TATTTTCGAT TCTTCGCCCA AGAAGAGAGG ACGTCCAAAG 
ACCAAAATGC CTCGATTAGA TGCACCAGCA CATGGCTGCA CCCCAAACAA GTTGGCACCT 
TCAAAATCCT TCTCCAGTCT GCTCAAGTCT AAGGAAGAGC AGGACCCCCC TGTTTTGCAC 
CCTGAAGTGG ATTTTAATCC ACCTAAAATG ATGGCAAGAA AGCGAGGGCG ACCCAAGCGC 
TCACCTCCAA CTCTTCCTCA AGAAACCCAG CCTCCGACTC TTGCTCCTGA GTCAGGGGAA 
GCATCAGTTG GGGAAAAACC TTTTCGTAAT AAGGGTAATG GGCAGCTCAT TATGAAGACG 
ATCATAGGCA AAATCAATAA AATGAAGACA GTAAAACGGA AGAGGCTCCT CAGCCAAATA 
CTGCTTGGGC CAAAACCAGC AGAGGAGCAG GAATGCTCAA GAAGCAGTGG GCCAACAGGG 
TCTCCGGTTG CTACAAAACC ACAGTCCCTG ACATCACTTG CTGCTACATT TGGTGGCAAA 
TTAGGGCCCC AGATTAATGT AAGCAAAAAG GGGACAATAT ATATGGGTAA AAGAAGGGGT 
CGTAAGCCAA AAACATCAAC TAATCCTTCC CCAGGTGCCC CCACACCCCC TCCTGAGCCT 
TTCTTATCAC CAAACACTTC ATCCCCTCAT CATCACCAGG TGCAGGCTTT ACAAAACCAA 
CCACACCAGT TGCCTCCATC TGAGGTGTTT CCTTCCCCAT CACTATCACA GTCAAGTGGG 
GGCCAAAGCC CCATTAGTGA TGCTAGCTTT GTAGAGCCTG GCTCAGTGCA TTTCTCATCT 
CACCCACACT GCAGTCATTC TCATCAGGTC CACCACTCAT TCTCATTTCC ACCGCCTACT 
TTTTCAGCCC CCTCTGCTCG ATCTGTAGCA AGTGCTGCAA CATCCACTGC ACACCCATCT 
CAGAAGAAGT CCTCTTGCCG GGGTTACCAT CATCACCACC ACCATTACCG GCAGCACTAT 
CATTATCGAA AATTTTCTCC TCCAAGGCCA CTATTGCCAA CGTCTCCAGC TCCACTGAGT 
GAGCTGAAAG AAGCCACACC TTCTCCAGTG AGTGAATCAC ACAGTGAAGA GACTGTACCC 
AGTGACAGTG GCATTGGAAC AGACAATAAT AGCACCTCTG ACCGTGGGGA AAAAGCTGGA 
AGTGCCAATG GCTTACCTGG TGTGGGGGTC TCACAGGGTA TGGCAGGTGG ACTCTTAATG 
CCTGGAGTCA TAGGTCCAGG TATTGGAACG GGAATTGGAC TTTCTTCGAG AGGTCGGAGG 
AGGCACTCAT CTTCAGTTCT TCTAGAACGT CCCTCACCTA CACCCTCACC TCTTGGAGTG 
AGAGCATCAC CTGACCCAAG AAGATCGCAC CCTGCTGCCC CATCTTCTGC TTTGGTTGGA 
CATAAAGAGA AACACAAGCA TAAGTGCAAG CGTCGTGGGC ATGGCTGCCC TAGCTATGAC 
AAACTAAAAA GGCAAAAGCG TAAACGCAAG AAGAAGTACC TGCAGCTACG GTCCCGCAGA 
CAAGACCCAG ATTTTTTGGC TGATTTAGAT GAGATATGTG TGCAGCTTAG TGATATACGA 
ATTTCTCATC GAACACCTAC ACTTCGTTTG GGCAGCAGCA TAGGTTTGGG TGCCGGGGCT 
GGTCCTGGCC GTGGGTCTGG CTTGGGAAGT ACCAGACCTA GCAGCAGCCT TGGAGGCGGA 
ACCAATCCAC CACCCCATCA CTATCTGCAT AGAGATCTAT TACCCACAAT ATTCAGGATA 
AATTTTAGTG GGTATTATTC ACCCCACCCA GCCTACCCAT GTGATTCACT TCATTACGTT 
CGTAAGCCAG ATCTTAAAAA GAAAAGAGGT CGACCACCTA AGCTTCGGGA ATCCATATCA 
GAAGTACCTT TTGTACCTGG TTTAGGCTTT CCCCTCTCAA GTGGAGGTTT CTATCACCCT 
TCTTATGGTG TCCCATACTC TTCTACCCCA CTGGGACTTG GTTACTATCG TGGTTATACC 
CCACCAAGTG CCCTGTACCC CCATCCTCAT CACCAGGCTC CCCACTCTGG CCCATCCCAC 
CATGTTCATT CACCATCTTT TCCTCCACCT CCACCCCCTT CTTATGTGCA TCATCATCAC 
CCATCCCACC TGCTTCTTAA CCCCTCCAAA TTTCACAAGA AGAAACATAA GCTACTTAGG 
CAAGAGTATT TAGGTGGTGG TCGTTCATCC ATGCTCTATC CTGCCATGTC ATCTGAGCTG 
TCATTTGGCT GGCACCACAA ACACAAGCAT AGGCACAAAC ACCGTGATCG CTGTGGAGAA 
GAGGAGGGTG ATGATGGTGG TGGAGTTTTA GGTGGTATAG GAGGCAGAGG TGTTGGAGGT 
GAGGGCTTGG GGGCCGGAAG AGGGGAGAGA GGGAGCGTAG AGTCCCTGCA GCACTGCCGG 
TTTGGACGAG ATGGCTCTGC TGACTCCCGA ACTAACAAAC AGTCAGCTTC TGCCAACTCC 
CCTTCCTCTT CCTCCACATC CTCTGCAGAA CGGTACAAGC GAAAAGAGGC CTCTATGTCT 
TGTCTTGGGC CCTCACGACT GTCTTTGGGC ACTGCAAGTC GGGGACATCA TCCTGTTGAC 
TCCTGGTTCA GAATGGGAAG TTCTGATACT GATTACTCCA AGCTCTCTAG AAACCAGTCT 
TTTTCGGGGT CTGGTGCATT CTCTGAAGGA CATGTGCAAA ATCCAGTGGA TTGCTCAGAG 
AGTGATGAGG AAGATGCTGC TTCCCCCATT GAAGAGCCAG AGATCAGCCA TCACACAAAC 
CTGTTTGCAT CTGCACTATC AAGGACCTCC CTCAAAGGAA GTCGCAGCAA AAAAGGTGGA 
CCATTAGAGA GTCCCAGTCT CTCACGGTTG GACAGGACGG TTAGAAGGGA TCGTTCAACA 
TCTTCAGAGA AAAGAGAAAT TGGGAATGTA TCTAGCTTGC AGAACAGAGT TGGCCAATCC 
AGTGTTCCTG ATGTTTCTGA GGGTCTCCAT CATCGGCACC ACCATCACCA CCACCCTCCT 
CTATCCCTCT CGCACGCTGC CTCTTGCCTG CCGCACTGCA CTCTAGACCA GCCTGGAAAC 
ACTGCACCAC TCCGGCCCTC CAGCCGCCAA GCTAGTCCAT CCACATGCTC CTCCCAGCCT 
GCATGCTGCC TTGATTCCCC ACCTTCACAT CTCTCCCACC GGCCCCCTCC CAAAAACAAC 
CTGCTTCATG TCAACAAGAT CCTCCGTGCA AAGAAGCTCC AAAGGCAAGC ACGTACAGGG 
AACAACATGG TGAAAAAACG GGGTCCAGGA CGTCCCCGCA AGAACCCGCT GCCCTCACCT 
CCTGCAACAC CCCCACCACA GACTTCCGAA CAAGACAACC ACCCATTACC TGAAAGGCAC 
CGAGCAACTG CACTCTGGGA GGCAGATACT GTAGTTGATG CCATTGAGGC TGTCGTACAG 
AACCAGCGCA AAAAGGGACG CAAGCGGAAG CACTGGGAAA GGGAGACTGA AGGAGGTGAT 
GAAGAAGAGG AGGATGCGGA GGAAGAAAGA CAGGTGGAGG ACGAAGAAGA AGAAGAAGAC 
GAAGAACAAG AACAACAGTC AGCTGCTAGA GAAGACGATG TAATGTCGAG AGTTAGAACA 
GAAGGAAGCA GGCAATGGTT GACCCAGGAA GAAGTGCACT GTTTTCGCAG TGCACTGGAG 
GGAAAACCTG ATGGCTCCCC TGAGCGCCCA GGCCCTGCTA CTTCGGAACC AACCCCAAAC 
CCATCAGTGA CCAGCCAACG TGAGAAGAGA GTTGCTAGAC CACCCAAAAA GAAGTTTCAA 
AAGGCGGGAC TTTACTCTGA TGTGTACAAG ACTGATGATC CTCGCAGTCA GCTGCTACAG 
TTAAAGAAAG AGAAGCTAGA ATACACTCCA GGTGAACATG ACTATGGCCT TCTTCCTGCT 
CCCATTCATG TTGGGAAATA CTTGAGACAG AAACGCATTG ATTTCCAGCT GCCTTACGAC 
ATCCTGTGGC TTTGGAAACA TGATCAGCTC TACAAGAGAC CTGACGTGCC TCTCTACAAG 
AAGATCAGAT CCAATGTCTA TGTGGATGTG AAGCCTCTTT CTGGCTATGA AGCCACTACC 
TGTAACTGCA GGTTGCCTGA CGACTCTAGT GAAAAGGGCT GTCAAGATGA TTGTCTAAAC 
AGGATGATTT ATGCCGAGTG CTCTCCAAGT ACGTGTCCGT GTTCCGATCA GTGCGATAAC 
CAGCGGATCC AGAAGCATGA GTGGGTTCAG TGCCTCGAGC GCTTCCGAGC TGAGGGAAAG 
GGCTGGGGCA TCCGGACCAA GCAGCCCCTT CGTGCCGGCC AATTTATCAT CGAGTACTTG 
GGAGAGGTGG TCAGCGAACA AGAGTTCAGG AGTCGCATGA TGGAGCAGTA CTTCTCTCAC 
AGCGGCCACT ACTGCCTGAA TCTGGACAGC GGCATGGTCA TCGATAGCTA CCGCATGGGC 
AACGAGGCTC GCTTTGTCAA CCACAGCTGC GAGCCCAACT GTGAGATGCA GAAATGGTCG 
GTGAACGGAG TGTACCGAAT TGGCCTGTTT GCTCTAAAAG ACATCAACAG CGGAACGGAA 
CTGACATATG ACTACAACTT CCACTCCTTC AACACAGAGG AACAACAAGT CTGCAAGTGT 
GGCTCAGAGG GTTGCCGAGG CATCATTGGC GGCAAGAGCA AGCGAATCAA TGGGCTGCCT 
GCTAAAGGGG CCGGATCTGG AGGCGGAGCC CGGAGACTTG GAAGATTGAA AGAGAAACGC 
AAGTCCAAAC ACTCACTGAA GAAGCGGGAG GAAGAGTCCA GTGACAGCAG CAAGTTTTAC 
CAGCACCTTT TAATGAAGCC CATGTCAAAC AGAGAGAGGA ACTTTGTGCT GAAACATCGG 
GTCTTTCTGC TGCGGAATTG GGAGAAGATG CGGGAGAAAC AGGAGCTTCT GAAGCGTGAG 
GGAGAGCGGG AGAGGGAGAA CAGCGGTCTG TCTCTGTACA CCCGCTGGGG AGGAGTCATC 
CGGGATGATG GAAACATCAA ATCAGATGTA TTTCTGACTC AGTTCTCAGC GCTGCAGACG 
TCCCGCTCTG TACGCACACG CAGACTTGCG GCAGCCGAGG AGAACACTGA AGTGACACGC 
ACCGCACGTC TGGCACACAT TTTCAAAGAG ATCTGCGATA TGATCACTAG CTACAAGGAT 
TCATCTGGTC AGCCTCTTGC TGCTCCGCTG CTTAACCTGC CTTCTAGGAA GAGGAACACA 
CAATACTATG AGAAGGTCAC CGATCCTCTA GATCTCAGCA CCATAGATAA GCAGATCCTC 
AGCGGACACT ACAAAACCGA TGAGGCGTTT GATGCGGACA TGCTCAAAGT GTTTCGCAAT 
GCTGAGAAGT ACTATGGGCG TAAGTCTGCT GTGGGCCGTG ACGTGTGCCG GCTGAGGAAG 
GCTTATTATG GTGCCCGTCA TGAGGCTGCT GTTCAGATCG ATGAGATTGT GGGTGAGACG 
GCAAGTGAAG CGGACAGCTC TGATTCGCTG GAGAGAGACC ACGCCCACCA CCATCACCAT 
GACGGAGGGG GCAGCCATGA TAAGGATGAT GATGTGATCC GCTGCATCTG TGGCATGTAC 
AAGGATGAGG GACTCATGAT TCAGTGCGAG AAGTGCATGG TGTGGCAGCA CTGTGATTGT 
ATGCGTCTGG AGGCGGATGT GGAGCATTAT CTCTGTGAAC AGTGTGACCC TCGTCCAGTG 
GACAGAGAGG TTCCAATGGT GCCTCAACCC AGCTATGCCC AATCAGGATT CATCTATTAC 
ATATGCCTGC TTCGCGATGA CTTACTGTTG CATCAGGGCG ATTGTGTGTA CCTCATGCGG 
GACAGCAGAC GCACAACAGA AGGTCAGCCG GTGCGTCAGT CATACCGCCT CCTGTCTCAC 
ATCAACAGAG ACAAACTGGA CATCTTCCGT ATCGAGAAAC TCTGGAAGAA TGAAAAAGGT 
GAGAGGTTTG CATTTGGTCA TCATTACTTC CGTCCTCATG AGACGCATCA CTCCCCTTCC 
CGCCGCTTCT ACCACAACGA GCTGTTTCGA GTGCCTCTGT ATGAGATCAT CCCGCTGGAG 
GCCGTGGTGG GCACCTGCTG CGTGCTGGAC CTCTACACAT ACTGCAAGGG TCGTCCAAAA 
GGGGTAAAGG AGCAGGACGT TTACATCTGC GACTACCGGC TGGATAAATC CGCACACCTG 
TTCTACAAGA TCCACCGCAA CCGTTACCCA GTGTGCACCA AGCCCTATGC GTTCAACCAC 
TTCCCCAAGA GACTGACGCC CAAGAGGGAC TTCTCTCCTC ACTATGTTCC AGATAACTAC 
AAGAGGAACG GCGGACGCTC TGCTTGGAAG AGCGAGCGGC CCAAAGATGA AGAACCATCA 
TCCTCCAGCA CGTCCTGCGA GCGTCTGTCC TCCAGCTTCC CTAGAGAAGG AGACGAGGAC 
GGAGGACGAG GAGCAGAAGG AGACGCGGAG GCCGCTCAAG AGGGATCCAG CACTCCGAGA 
CCTCAGAGAT CCTCGCCACT GGAGAGCAGG ACGGCCGGAG AGGAGGATGA GGCGGAGGAG 
GAAGAGGACA GAGAGAGGAG GAGAGAGATG CAGGAGGAGG AGGAGGAGGA GGGATGCAGA 
GAGAAAGCAG GAGCGCAGGA GCCCATAGAC GCACAGTGCT CCTCCTCCAT CTCCTCTTCT 
CCTCTTCATC CCGCCATTCT GGGCAGGAGA GAAGCGCAGA GAGAGCGGCT CAACAAGATC 
CTGCTCGACC TGCTGCACCG CGCGCCCACT AAGAACGCGA TAGATGTGAC GTATCTTCTT 
GAGGAAGGCT CGGGTCGTCG CCTTCGCAGA CGGACGCTTG GACTGAGCGA ATTCATTTGC 
AGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333336.1
CDS	429..9317
Translation	MDQRTQGGPSTPPPLPANAPPAEREKEGVGGKKDDEEEKKKREKLRDGAQNEASGAESGTGDQPQQPTQFSVKETSYSEGNVKLKIGLQAKRMKKPPKILENYVCRPAFRATVRHSGGRGGGRANRGAGASDANSNSTSTSNGKEGEKKPSVNRGASQSAAAPAASTPSPSLPPASINTVTPVNGNAPAKRGPPKLACKSEGKADVKSVASSEKPLNLHRPTTESKSHSSGKKNHVPQTNSAPAPSPPAVPPAATSQQDPKIDGILKTPQCNFLEGQKEQERGISSWGTPTVTEKLAQLIATCPPSKCPKPKARKVSPAPAHSSSPPLISNLQRPERAMANRNTYSRAVHLAQPPPVSRPPGRPYGSKNKDSILEKFSGTSKKEESDGLSKGVSGSSSTTTTTTSSALSQNSSRLTTTTSTNESTDINNTDGMKSQSRLGHCPSHSSSAAASPPFSSSSSVERIGSTSCNIGLPGSLTSRPKASPSRIEDFSEKDKERDREKDTDSPRDLSKGITPAITGKQDAKERCSLSAPRNDRGKSSSPVKRSPNQESRVSGVPSPAESTKCTISPSYPSGASRSPPPLCDDTGAISPASPSEQDSKPLKKRRGRRPRWTKAVSRTHKSLHESPSNHQQIPTPMPSGLSFSSPIRESVEQPLSDSYTSPEDSDIFDSSPKKRGRPKTKMPRLDAPAHGCTPNKLAPSKSFSSLLKSKEEQDPPVLHPEVDFNPPKMMARKRGRPKRSPPTLPQETQPPTLAPESGEASVGEKPFRNKGNGQLIMKTIIGKINKMKTVKRKRLLSQILLGPKPAEEQECSRSSGPTGSPVATKPQSLTSLAATFGGKLGPQINVSKKGTIYMGKRRGRKPKTSTNPSPGAPTPPPEPFLSPNTSSPHHHQVQALQNQPHQLPPSEVFPSPSLSQSSGGQSPISDASFVEPGSVHFSSHPHCSHSHQVHHSFSFPPPTFSAPSARSVASAATSTAHPSQKKSSCRGYHHHHHHYRQHYHYRKFSPPRPLLPTSPAPLSELKEATPSPVSESHSEETVPSDSGIGTDNNSTSDRGEKAGSANGLPGVGVSQGMAGGLLMPGVIGPGIGTGIGLSSRGRRRHSSSVLLERPSPTPSPLGVRASPDPRRSHPAAPSSALVGHKEKHKHKCKRRGHGCPSYDKLKRQKRKRKKKYLQLRSRRQDPDFLADLDEICVQLSDIRISHRTPTLRLGSSIGLGAGAGPGRGSGLGSTRPSSSLGGGTNPPPHHYLHRDLLPTIFRINFSGYYSPHPAYPCDSLHYVRKPDLKKKRGRPPKLRESISEVPFVPGLGFPLSSGGFYHPSYGVPYSSTPLGLGYYRGYTPPSALYPHPHHQAPHSGPSHHVHSPSFPPPPPPSYVHHHHPSHLLLNPSKFHKKKHKLLRQEYLGGGRSSMLYPAMSSELSFGWHHKHKHRHKHRDRCGEEEGDDGGGVLGGIGGRGVGGEGLGAGRGERGSVESLQHCRFGRDGSADSRTNKQSASANSPSSSSTSSAERYKRKEASMSCLGPSRLSLGTASRGHHPVDSWFRMGSSDTDYSKLSRNQSFSGSGAFSEGHVQNPVDCSESDEEDAASPIEEPEISHHTNLFASALSRTSLKGSRSKKGGPLESPSLSRLDRTVRRDRSTSSEKREIGNVSSLQNRVGQSSVPDVSEGLHHRHHHHHHPPLSLSHAASCLPHCTLDQPGNTAPLRPSSRQASPSTCSSQPACCLDSPPSHLSHRPPPKNNLLHVNKILRAKKLQRQARTGNNMVKKRGPGRPRKNPLPSPPATPPPQTSEQDNHPLPERHRATALWEADTVVDAIEAVVQNQRKKGRKRKHWERETEGGDEEEEDAEEERQVEDEEEEEDEEQEQQSAAREDDVMSRVRTEGSRQWLTQEEVHCFRSALEGKPDGSPERPGPATSEPTPNPSVTSQREKRVARPPKKKFQKAGLYSDVYKTDDPRSQLLQLKKEKLEYTPGEHDYGLLPAPIHVGKYLRQKRIDFQLPYDILWLWKHDQLYKRPDVPLYKKIRSNVYVDVKPLSGYEATTCNCRLPDDSSEKGCQDDCLNRMIYAECSPSTCPCSDQCDNQRIQKHEWVQCLERFRAEGKGWGIRTKQPLRAGQFIIEYLGEVVSEQEFRSRMMEQYFSHSGHYCLNLDSGMVIDSYRMGNEARFVNHSCEPNCEMQKWSVNGVYRIGLFALKDINSGTELTYDYNFHSFNTEEQQVCKCGSEGCRGIIGGKSKRINGLPAKGAGSGGGARRLGRLKEKRKSKHSLKKREEESSDSSKFYQHLLMKPMSNRERNFVLKHRVFLLRNWEKMREKQELLKREGERERENSGLSLYTRWGGVIRDDGNIKSDVFLTQFSALQTSRSVRTRRLAAAEENTEVTRTARLAHIFKEICDMITSYKDSSGQPLAAPLLNLPSRKRNTQYYEKVTDPLDLSTIDKQILSGHYKTDEAFDADMLKVFRNAEKYYGRKSAVGRDVCRLRKAYYGARHEAAVQIDEIVGETASEADSSDSLERDHAHHHHHDGGGSHDKDDDVIRCICGMYKDEGLMIQCEKCMVWQHCDCMRLEADVEHYLCEQCDPRPVDREVPMVPQPSYAQSGFIYYICLLRDDLLLHQGDCVYLMRDSRRTTEGQPVRQSYRLLSHINRDKLDIFRIEKLWKNEKGERFAFGHHYFRPHETHHSPSRRFYHNELFRVPLYEIIPLEAVVGTCCVLDLYTYCKGRPKGVKEQDVYICDYRLDKSAHLFYKIHRNRYPVCTKPYAFNHFPKRLTPKRDFSPHYVPDNYKRNGGRSAWKSERPKDEEPSSSSTSCERLSSSFPREGDEDGGRGAEGDAEAAQEGSSTPRPQRSSPLESRTAGEEDEAEEEEDRERRREMQEEEEEEGCREKAGAQEPIDAQCSSSISSSPLHPAILGRREAQRERLNKILLDLLHRAPTKNAIDVTYLLEEGSGRRLRRRTLGLSEFICRK

Target ORF information:

RefSeq Version	NM_001346407.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ash1 (absent, small, or homeotic)-like (Drosophila) (ash1l), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346407.1

ORF Insert Sequence:

ATGGATCAGA GGACACAGGG GGGGCCTTCT ACGCCGCCCC CACTCCCTGC CAATGCCCCC 
CCAGCAGAAC GTGAAAAAGA AGGTGTAGGA GGAAAGAAGG ACGATGAAGA AGAGAAAAAG 
AAACGGGAGA AACTAAGAGA TGGGGCACAG AATGAAGCTA GTGGTGCTGA AAGTGGGACT 
GGTGACCAGC CGCAACAACC CACGCAGTTT TCGGTCAAGG AGACCAGCTA CTCTGAGGGT 
AACGTTAAGC TCAAAATAGG CCTGCAGGCC AAACGCATGA AGAAACCTCC TAAGATCCTG 
GAGAACTATG TCTGCCGACC AGCCTTCCGT GCCACTGTAA GGCATAGTGG AGGTAGAGGC 
GGAGGACGTG CTAATCGTGG GGCTGGAGCC AGTGATGCGA ACAGCAACTC TACTAGCACA 
AGTAATGGAA AGGAAGGGGA GAAAAAGCCC TCTGTCAACC GAGGAGCATC TCAGTCAGCT 
GCTGCTCCTG CTGCTTCTAC TCCCTCACCC TCTCTTCCTC CTGCTTCCAT CAACACTGTC 
ACTCCAGTGA ATGGAAATGC TCCAGCAAAA AGGGGTCCTC CAAAACTTGC CTGCAAATCA 
GAGGGAAAGG CTGACGTAAA ATCTGTTGCT TCATCAGAGA AACCTCTTAA TCTCCATCGC 
CCCACGACAG AAAGTAAATC ACACTCCTCT GGAAAGAAAA ACCATGTTCC ACAAACAAAC 
TCTGCCCCTG CCCCATCTCC ACCAGCAGTG CCCCCAGCTG CCACTTCGCA ACAGGACCCT 
AAAATAGATG GAATCTTAAA AACGCCTCAA TGCAATTTTT TGGAGGGGCA GAAGGAACAA 
GAAAGAGGGA TTTCTTCTTG GGGTACCCCT ACAGTAACTG AAAAACTTGC TCAGCTCATA 
GCTACATGCC CACCTTCAAA GTGCCCTAAG CCAAAAGCCC GAAAGGTATC GCCAGCTCCA 
GCACACAGTA GCTCACCTCC GTTAATTTCT AATCTGCAGC GCCCTGAACG GGCAATGGCT 
AATAGAAACA CCTATTCCAG GGCGGTTCAC CTCGCCCAAC CACCACCTGT ATCACGGCCC 
CCTGGTCGCC CATATGGGTC TAAAAACAAA GACAGTATCC TTGAGAAATT TTCTGGAACT 
TCTAAAAAAG AGGAATCAGA TGGACTTTCA AAAGGGGTCT CCGGTAGCAG CAGCACAACC 
ACCACCACCA CTAGTTCAGC ACTGTCTCAA AACAGCAGTC GCTTGACGAC AACAACATCC 
ACAAATGAAA GCACTGACAT TAACAACACA GATGGAATGA AATCTCAGTC TCGTCTTGGG 
CATTGCCCAT CTCATTCTTC ATCCGCAGCT GCTTCACCTC CATTTTCTTC TTCATCCTCC 
GTAGAGAGAA TAGGAAGTAC AAGCTGTAAC ATAGGTTTGC CAGGCTCTCT AACCTCACGC 
CCTAAAGCAA GCCCCTCTCG AATAGAGGAT TTTAGTGAGA AAGACAAAGA ACGTGACCGG 
GAAAAGGACA CAGACAGTCC AAGAGATTTA AGTAAAGGTA TCACTCCAGC TATAACTGGG 
AAGCAGGATG CAAAAGAAAG GTGTTCTTTA TCAGCTCCTC GAAATGACAG GGGTAAGAGC 
TCAAGCCCAG TCAAACGTAG TCCCAATCAG GAAAGTCGCG TTAGTGGGGT ACCAAGTCCT 
GCTGAGTCCA CCAAATGCAC TATTTCTCCT TCATACCCTT CTGGTGCCAG CAGGAGCCCA 
CCACCACTAT GCGATGACAC CGGGGCTATT TCTCCTGCAT CACCATCTGA GCAGGATTCA 
AAACCTCTTA AGAAGCGACG AGGACGACGA CCTCGCTGGA CGAAGGCAGT GAGTCGCACC 
CACAAGTCTT TACATGAGTC TCCTTCTAAC CATCAGCAAA TCCCCACACC AATGCCCTCA 
GGGCTTTCTT TCTCATCCCC TATTCGGGAG TCTGTGGAGC AGCCTCTCAG TGACAGTTAC 
ACATCCCCAG AGGATTCTGA TATTTTCGAT TCTTCGCCCA AGAAGAGAGG ACGTCCAAAG 
ACCAAAATGC CTCGATTAGA TGCACCAGCA CATGGCTGCA CCCCAAACAA GTTGGCACCT 
TCAAAATCCT TCTCCAGTCT GCTCAAGTCT AAGGAAGAGC AGGACCCCCC TGTTTTGCAC 
CCTGAAGTGG ATTTTAATCC ACCTAAAATG ATGGCAAGAA AGCGAGGGCG ACCCAAGCGC 
TCACCTCCAA CTCTTCCTCA AGAAACCCAG CCTCCGACTC TTGCTCCTGA GTCAGGGGAA 
GCATCAGTTG GGGAAAAACC TTTTCGTAAT AAGGGTAATG GGCAGCTCAT TATGAAGACG 
ATCATAGGCA AAATCAATAA AATGAAGACA GTAAAACGGA AGAGGCTCCT CAGCCAAATA 
CTGCTTGGGC CAAAACCAGC AGAGGAGCAG GAATGCTCAA GAAGCAGTGG GCCAACAGGG 
TCTCCGGTTG CTACAAAACC ACAGTCCCTG ACATCACTTG CTGCTACATT TGGTGGCAAA 
TTAGGGCCCC AGATTAATGT AAGCAAAAAG GGGACAATAT ATATGGGTAA AAGAAGGGGT 
CGTAAGCCAA AAACATCAAC TAATCCTTCC CCAGGTGCCC CCACACCCCC TCCTGAGCCT 
TTCTTATCAC CAAACACTTC ATCCCCTCAT CATCACCAGG TGCAGGCTTT ACAAAACCAA 
CCACACCAGT TGCCTCCATC TGAGGTGTTT CCTTCCCCAT CACTATCACA GTCAAGTGGG 
GGCCAAAGCC CCATTAGTGA TGCTAGCTTT GTAGAGCCTG GCTCAGTGCA TTTCTCATCT 
CACCCACACT GCAGTCATTC TCATCAGGTC CACCACTCAT TCTCATTTCC ACCGCCTACT 
TTTTCAGCCC CCTCTGCTCG ATCTGTAGCA AGTGCTGCAA CATCCACTGC ACACCCATCT 
CAGAAGAAGT CCTCTTGCCG GGGTTACCAT CATCACCACC ACCATTACCG GCAGCACTAT 
CATTATCGAA AATTTTCTCC TCCAAGGCCA CTATTGCCAA CGTCTCCAGC TCCACTGAGT 
GAGCTGAAAG AAGCCACACC TTCTCCAGTG AGTGAATCAC ACAGTGAAGA GACTGTACCC 
AGTGACAGTG GCATTGGAAC AGACAATAAT AGCACCTCTG ACCGTGGGGA AAAAGCTGGA 
AGTGCCAATG GCTTACCTGG TGTGGGGGTC TCACAGGGTA TGGCAGGTGG ACTCTTAATG 
CCTGGAGTCA TAGGTCCAGG TATTGGAACG GGAATTGGAC TTTCTTCGAG AGGTCGGAGG 
AGGCACTCAT CTTCAGTTCT TCTAGAACGT CCCTCACCTA CACCCTCACC TCTTGGAGTG 
AGAGCATCAC CTGACCCAAG AAGATCGCAC CCTGCTGCCC CATCTTCTGC TTTGGTTGGA 
CATAAAGAGA AACACAAGCA TAAGTGCAAG CGTCGTGGGC ATGGCTGCCC TAGCTATGAC 
AAACTAAAAA GGCAAAAGCG TAAACGCAAG AAGAAGTACC TGCAGCTACG GTCCCGCAGA 
CAAGACCCAG ATTTTTTGGC TGATTTAGAT GAGATATGTG TGCAGCTTAG TGATATACGA 
ATTTCTCATC GAACACCTAC ACTTCGTTTG GGCAGCAGCA TAGGTTTGGG TGCCGGGGCT 
GGTCCTGGCC GTGGGTCTGG CTTGGGAAGT ACCAGACCTA GCAGCAGCCT TGGAGGCGGA 
ACCAATCCAC CACCCCATCA CTATCTGCAT AGAGATCTAT TACCCACAAT ATTCAGGATA 
AATTTTAGTG GGTATTATTC ACCCCACCCA GCCTACCCAT GTGATTCACT TCATTACGTT 
CGTAAGCCAG ATCTTAAAAA GAAAAGAGGT CGACCACCTA AGCTTCGGGA ATCCATATCA 
GAAGTACCTT TTGTACCTGG TTTAGGCTTT CCCCTCTCAA GTGGAGGTTT CTATCACCCT 
TCTTATGGTG TCCCATACTC TTCTACCCCA CTGGGACTTG GTTACTATCG TGGTTATACC 
CCACCAAGTG CCCTGTACCC CCATCCTCAT CACCAGGCTC CCCACTCTGG CCCATCCCAC 
CATGTTCATT CACCATCTTT TCCTCCACCT CCACCCCCTT CTTATGTGCA TCATCATCAC 
CCATCCCACC TGCTTCTTAA CCCCTCCAAA TTTCACAAGA AGAAACATAA GCTACTTAGG 
CAAGAGTATT TAGGTGGTGG TCGTTCATCC ATGCTCTATC CTGCCATGTC ATCTGAGCTG 
TCATTTGGCT GGCACCACAA ACACAAGCAT AGGCACAAAC ACCGTGATCG CTGTGGAGAA 
GAGGAGGGTG ATGATGGTGG TGGAGTTTTA GGTGGTATAG GAGGCAGAGG TGTTGGAGGT 
GAGGGCTTGG GGGCCGGAAG AGGGGAGAGA GGGAGCGTAG AGTCCCTGCA GCACTGCCGG 
TTTGGACGAG ATGGCTCTGC TGACTCCCGA ACTAACAAAC AGTCAGCTTC TGCCAACTCC 
CCTTCCTCTT CCTCCACATC CTCTGCAGAA CGGTACAAGC GAAAAGAGGC CTCTATGTCT 
TGTCTTGGGC CCTCACGACT GTCTTTGGGC ACTGCAAGTC GGGGACATCA TCCTGTTGAC 
TCCTGGTTCA GAATGGGAAG TTCTGATACT GATTACTCCA AGCTCTCTAG AAACCAGTCT 
TTTTCGGGGT CTGGTGCATT CTCTGAAGGA CATGTGCAAA ATCCAGTGGA TTGCTCAGAG 
AGTGATGAGG AAGATGCTGC TTCCCCCATT GAAGAGCCAG AGATCAGCCA TCACACAAAC 
CTGTTTGCAT CTGCACTATC AAGGACCTCC CTCAAAGGAA GTCGCAGCAA AAAAGGTGGA 
CCATTAGAGA GTCCCAGTCT CTCACGGTTG GACAGGACGG TTAGAAGGGA TCGTTCAACA 
TCTTCAGAGA AAAGAGAAAT TGGGAATGTA TCTAGCTTGC AGAACAGAGT TGGCCAATCC 
AGTGTTCCTG ATGTTTCTGA GGGTCTCCAT CATCGGCACC ACCATCACCA CCACCCTCCT 
CTATCCCTCT CGCACGCTGC CTCTTGCCTG CCGCACTGCA CTCTAGACCA GCCTGGAAAC 
ACTGCACCAC TCCGGCCCTC CAGCCGCCAA GCTAGTCCAT CCACATGCTC CTCCCAGCCT 
GCATGCTGCC TTGATTCCCC ACCTTCACAT CTCTCCCACC GGCCCCCTCC CAAAAACAAC 
CTGCTTCATG TCAACAAGAT CCTCCGTGCA AAGAAGCTCC AAAGGCAAGC ACGTACAGGG 
AACAACATGG TGAAAAAACG GGGTCCAGGA CGTCCCCGCA AGAACCCGCT GCCCTCACCT 
CCTGCAACAC CCCCACCACA GACTTCCGAA CAAGACAACC ACCCATTACC TGAAAGGCAC 
CGAGCAACTG CACTCTGGGA GGCAGATACT GTAGTTGATG CCATTGAGGC TGTCGTACAG 
AACCAGCGCA AAAAGGGACG CAAGCGGAAG CACTGGGAAA GGGAGACTGA AGGAGGTGAT 
GAAGAAGAGG AGGATGCGGA GGAAGAAAGA CAGGTGGAGG ACGAAGAAGA AGAAGAAGAC 
GAAGAACAAG AACAACAGTC AGCTGCTAGA GAAGACGATG TAATGTCGAG AGTTAGAACA 
GAAGGAAGCA GGCAATGGTT GACCCAGGAA GAAGTGCACT GTTTTCGCAG TGCACTGGAG 
GGAAAACCTG ATGGCTCCCC TGAGCGCCCA GGCCCTGCTA CTTCGGAACC AACCCCAAAC 
CCATCAGTGA CCAGCCAACG TGAGAAGAGA GTTGCTAGAC CACCCAAAAA GAAGTTTCAA 
AAGGCGGGAC TTTACTCTGA TGTGTACAAG ACTGATGATC CTCGCAGTCA GCTGCTACAG 
TTAAAGAAAG AGAAGCTAGA ATACACTCCA GGTGAACATG ACTATGGCCT TCTTCCTGCT 
CCCATTCATG TTGGGAAATA CTTGAGACAG AAACGCATTG ATTTCCAGCT GCCTTACGAC 
ATCCTGTGGC TTTGGAAACA TGATCAGCTC TACAAGAGAC CTGACGTGCC TCTCTACAAG 
AAGATCAGAT CCAATGTCTA TGTGGATGTG AAGCCTCTTT CTGGCTATGA AGCCACTACC 
TGTAACTGCA GGTTGCCTGA CGACTCTAGT GAAAAGGGCT GTCAAGATGA TTGTCTAAAC 
AGGATGATTT ATGCCGAGTG CTCTCCAAGT ACGTGTCCGT GTTCCGATCA GTGCGATAAC 
CAGCGGATCC AGAAGCATGA GTGGGTTCAG TGCCTCGAGC GCTTCCGAGC TGAGGGAAAG 
GGCTGGGGCA TCCGGACCAA GCAGCCCCTT CGTGCCGGCC AATTTATCAT CGAGTACTTG 
GGAGAGGTGG TCAGCGAACA AGAGTTCAGG AGTCGCATGA TGGAGCAGTA CTTCTCTCAC 
AGCGGCCACT ACTGCCTGAA TCTGGACAGC GGCATGGTCA TCGATAGCTA CCGCATGGGC 
AACGAGGCTC GCTTTGTCAA CCACAGCTGC GAGCCCAACT GTGAGATGCA GAAATGGTCG 
GTGAACGGAG TGTACCGAAT TGGCCTGTTT GCTCTAAAAG ACATCAACAG CGGAACGGAA 
CTGACATATG ACTACAACTT CCACTCCTTC AACACAGAGG AACAACAAGT CTGCAAGTGT 
GGCTCAGAGG GTTGCCGAGG CATCATTGGC GGCAAGAGCA AGCGAATCAA TGGGCTGCCT 
GCTAAAGGGG CCGGATCTGG AGGCGGAGCC CGGAGACTTG GAAGATTGAA AGAGAAACGC 
AAGTCCAAAC ACTCACTGAA GAAGCGGGAG GAAGAGTCCA GTGACAGCAG CAAGTTTTAC 
CAGCACCTTT TAATGAAGCC CATGTCAAAC AGAGAGAGGA ACTTTGTGCT GAAACATCGG 
GTCTTTCTGC TGCGGAATTG GGAGAAGATG CGGGAGAAAC AGGAGCTTCT GAAGCGTGAG 
GGAGAGCGGG AGAGGGAGAA CAGCGGTCTG TCTCTGTACA CCCGCTGGGG AGGAGTCATC 
CGGGATGATG GAAACATCAA ATCAGATGTA TTTCTGACTC AGTTCTCAGC GCTGCAGACG 
TCCCGCTCTG TACGCACACG CAGACTTGCG GCAGCCGAGG AGAACACTGA AGTGACACGC 
ACCGCACGTC TGGCACACAT TTTCAAAGAG ATCTGCGATA TGATCACTAG CTACAAGGAT 
TCATCTGGTC AGCCTCTTGC TGCTCCGCTG CTTAACCTGC CTTCTAGGAA GAGGAACACA 
CAATACTATG AGAAGGTCAC CGATCCTCTA GATCTCAGCA CCATAGATAA GCAGATCCTC 
AGCGGACACT ACAAAACCGA TGAGGCGTTT GATGCGGACA TGCTCAAAGT GTTTCGCAAT 
GCTGAGAAGT ACTATGGGCG TAAGTCTGCT GTGGGCCGTG ACGTGTGCCG GCTGAGGAAG 
GCTTATTATG GTGCCCGTCA TGAGGCTGCT GTTCAGATCG ATGAGATTGT GGGTGAGACG 
GCAAGTGAAG CGGACAGCTC TGATTCGCTG GAGAGAGACC ACGCCCACCA CCATCACCAT 
GACGGAGGGG GCAGCCATGA TAAGGATGAT GATGTGATCC GCTGCATCTG TGGCATGTAC 
AAGGATGAGG GACTCATGAT TCAGTGCGAG AAGTGCATGG TGTGGCAGCA CTGTGATTGT 
ATGCGTCTGG AGGCGGATGT GGAGCATTAT CTCTGTGAAC AGTGTGACCC TCGTCCAGTG 
GACAGAGAGG TTCCAATGGT GCCTCAACCC AGCTATGCCC AATCAGGATT CATCTATTAC 
ATATGCCTGC TTCGCGATGA CTTACTGTTG CATCAGGGCG ATTGTGTGTA CCTCATGCGG 
GACAGCAGAC GCACAACAGA AGGTCAGCCG GTGCGTCAGT CATACCGCCT CCTGTCTCAC 
ATCAACAGAG ACAAACTGGA CATCTTCCGT ATCGAGAAAC TCTGGAAGAA TGAAAAAGGT 
GAGAGGTTTG CATTTGGTCA TCATTACTTC CGTCCTCATG AGACGCATCA CTCCCCTTCC 
CGCCGCTTCT ACCACAACGA GCTGTTTCGA GTGCCTCTGT ATGAGATCAT CCCGCTGGAG 
GCCGTGGTGG GCACCTGCTG CGTGCTGGAC CTCTACACAT ACTGCAAGGG TCGTCCAAAA 
GGGGTAAAGG AGCAGGACGT TTACATCTGC GACTACCGGC TGGATAAATC CGCACACCTG 
TTCTACAAGA TCCACCGCAA CCGTTACCCA GTGTGCACCA AGCCCTATGC GTTCAACCAC 
TTCCCCAAGA GACTGACGCC CAAGAGGGAC TTCTCTCCTC ACTATGTTCC AGATAACTAC 
AAGAGGAACG GCGGACGCTC TGCTTGGAAG AGCGAGCGGC CCAAAGATGA AGAACCATCA 
TCCTCCAGCA CGTCCTGCGA GCGTCTGTCC TCCAGCTTCC CTAGAGAAGG AGACGAGGAC 
GGAGGACGAG GAGCAGAAGG AGACGCGGAG GCCGCTCAAG AGGGATCCAG CACTCCGAGA 
CCTCAGAGAT CCTCGCCACT GGAGAGCAGG ACGGCCGGAG AGGAGGATGA GGCGGAGGAG 
GAAGAGGACA GAGAGAGGAG GAGAGAGATG CAGGAGGAGG AGGAGGAGGA GGGATGCAGA 
GAGAAAGCAG GAGCGCAGGA GCCCATAGAC GCACAGTGCT CCTCCTCCAT CTCCTCTTCT 
CCTCTTCATC CCGCCATTCT GGGCAGGAGA GAAGCGCAGA GAGAGCGGCT CAACAAGATC 
CTGCTCGACC TGCTGCACCG CGCGCCCACT AAGAACGCGA TAGATGTGAC GTATCTTCTT 
GAGGAAGGCT CGGGTCGTCG CCTTCGCAGA CGGACGCTTG GACTGAGCGA ATTCATTTGC 
AGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ash1l ( XM_005159483.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159540.1 Danio rerio ash1 (absent, small, or homeotic)-like (Drosophila) (ash1l), transcript variant X1, mRNA.

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CloneID	ODa23935
Clone ID Related Accession (Same CDS sequence)	XM_005159483.3 , XM_005159484.3 , XM_005159484.4 , XM_005159483.4
Accession Version	XM_005159483.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8967bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	histone-lysine N-methyltransferase ASH1L isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159483.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCAGA GGACACAGGG GGGGCCTTCT ACGCCGCCCC CACTCCCTGC CAATGCCCCC 
CCAGCAGAAC GTGAAAAAGA AGGTGTAGGA GGAAAGAAGG ACGATGAAGA AGAGAAAAAG 
AAACGGGAGA AACTAAGAGA TGGGGCACAG AATGAAGCTA GTGGTGCTGA AAGTGGGACT 
GGTGACCAGC CGCAACAACC CACGCAGTTT TCGGTCAAGG AGACCAGCTA CTCTGAGGGT 
AACGTTAAGC TCAAAATAGG CCTGCAGGCC AAACGCATGA AGAAACCTCC TAAGATCCTG 
GAGAACTATG TCTGCCGACC AGCCTTCCGT GCCACTGTAA GGCATAGTGG AGGTAGAGGC 
GGAGGACGTG CTAATCGTGG GGCTGGAGCC AGTGATGCGA ACAGCAACTC TACTAGCACA 
AGTAATGGAA AGGAAGGGGA GAAAAAGCCC TCTGTCAACC GAGGAGCATC TCAGTCAGCT 
GCTGCTCCTG CTGCTTCTAC TCCCTCACCC TCTCTTCCTC CTGCTTCCAT CAACACTGTC 
ACTCCAGTGA ATGGAAATGC TCCAGCAAAA AGGGGTCCTC CAAAACTTGC CTGCAAATCA 
GAGGGAAAGG CTGACGTAAA ATCTGTTGCT TCATCAGAGA AACCTCTTAA TCTCCATCGC 
CCCACGACAG AAAGTAAATC ACACTCCTCT GGAAAGAAAA ACCATGTTCC ACAAACAAAC 
TCTGCCCCTG CCCCATCTCC ACCAGCAGTG CCCCCAGCTG CCACTTCGCA ACAGGACCCT 
AAAATAGATG GAATCTTAAA AACGCCTCAA TGCAATTTTT TGGAGGGGCA GAAGGAACAA 
GAAAGAGGGA TTTCTTCTTG GGGTACCCCT ACAGTAACTG AAAAACTTGC TCAGCTCATA 
GCTACATGCC CACCTTCAAA GTGCCCTAAG CCAAAAGCCC GAAAGGTATC GCCAGCTCCA 
GCACACAGTA GCTCACCTCC GTTAATTTCT AATCTGCAGC GCCCTGAACG GGCAATGGCT 
AATAGAAACA CCTATTCCAG GGCGGTTCAC CTCGCCCAAC CACCACCTGT ATCACGGCCC 
CCTGGTCGCC CATATGGGTC TAAAAACAAA GACAGTATCC TTGAGAAATT TTCTGGAACT 
TCTAAAAAAG AGGAATCAGA TGGACTTTCA AAAGGGGTCT CCGGTAGCAG CAGCACAACC 
ACCACCACCA CTAGTTCAGC ACTGTCTCAA AACAGCAGTC GCTTGACGAC AACAACATCC 
ACAAATGAAA GCACTGACAT TAACAACACA GATGGAATGA AATCTCAGTC TCGTCTTGGG 
CATTGCCCAT CTCATTCTTC ATCCGCAGCT GCTTCACCTC CATTTTCTTC TTCATCCTCC 
GTAGAGAGAA TAGGAAGTAC AAGCTGTAAC ATAGGTTTGC CAGGCTCTCT AACCTCACGC 
CCTAAAGCAA GCCCCTCTCG AATAGAGGAT TTTAGTGAGA AAGACAAAGA ACGTGACCGG 
GAAAAGGACA CAGACAGTCC AAGAGATTTA AGTAAAGGTA TCACTCCAGC TATAACTGGG 
AAGCAGGATG CAAAAGAAAG GTGTTCTTTA TCAGCTCCTC GAAATGACAG GGGTAAGAGC 
TCAAGCCCAG TCAAACGTAG TCCCAATCAG GAAAGTCGCG TTAGTGGGGT ACCAAGTCCT 
GCTGAGTCCA CCAAATGCAC TATTTCTCCT TCATACCCTT CTGGTGCCAG CAGGAGCCCA 
CCACCACTAT GCGATGACAC CGGGGCTATT TCTCCTGCAT CACCATCTGA GCAGGATTCA 
AAACCTCTTA AGAAGCGACG AGGACGACGA CCTCGCTGGA CGAAGGCAGT GAGTCGCACC 
CACAAGTCTT TACATGAGTC TCCTTCTAAC CATCAGCAAA TCCCCACACC AATGCCCTCA 
GGGCTTTCTT TCTCATCCCC TATTCGGGAG TCTGTGGAGC AGCCTCTCAG TGACAGTTAC 
ACATCCCCAG AGGATTCTGA TATTTTCGAT TCTTCGCCCA AGAAGAGAGG ACGTCCAAAG 
ACCAAAATGC CTCGATTAGA TGCACCAGCA CATGGCTGCA CCCCAAACAA GTTGGCACCT 
TCAAAATCCT TCTCCAGTCT GCTCAAGTCT AAGGAAGAGC AGGACCCCCC TGTTTTGCAC 
CCTGAAGTGG ATTTTAATCC ACCTAAAATG ATGGCAAGAA AGCGAGGGCG ACCCAAGCGC 
TCACCTCCAA CTCTTCCTCA AGAAACCCAG CCTCCGACTC TTGCTCCTGA GTCAGGGGAA 
GCATCAGTTG GGGAAAAACC TTTTCGTAAT AAGGGTAATG GGCAGCTCAT TATGAAGACG 
ATCATAGGCA AAATCAATAA AATGAAGACA GTAAAACGGA AGAGGCTCCT CAGCCAAATA 
CTGCTTGGGC CAAAACCAGC AGAGGAGCAG GAATGCTCAA GAAGCAGTGG GCCAACAGGG 
TCTCCGGTTG CTACAAAACC ACAGTCCCTG ACATCACTTG CTGCTACATT TGGTGGCAAA 
TTAGGGCCCC AGATTAATGT AAGCAAAAAG GGGACAATAT ATATGGGTAA AAGAAGGGGT 
CGTAAGCCAA AAACATCAAC TAATCCTTCC CCAGGTGCCC CCACACCCCC TCCTGAGCCT 
TTCTTATCAC CAAACACTTC ATCCCCTCAT CATCACCAGG TGCAGGCTTT ACAAAACCAA 
CCACACCAGT TGCCTCCATC TGAGGTGTTT CCTTCCCCAT CACTATCACA GTCAAGTGGG 
GGCCAAAGCC CCATTAGTGA TGCTAGCTTT GTAGAGCCTG GCTCAGTGCA TTTCTCATCT 
CACCCACACT GCAGTCATTC TCATCAGGTC CACCACTCAT TCTCATTTCC ACCGCCTACT 
TTTTCAGCCC CCTCTGCTCG ATCTGTAGCA AGTGCTGCAA CATCCACTGC ACACCCATCT 
CAGAAGAAGT CCTCTTGCCG GGGTTACCAT CATCACCACC ACCATTACCG GCAGCACTAT 
CATTATCGAA AATTTTCTCC TCCAAGGCCA CTATTGCCAA CGTCTCCAGC TCCACTGAGT 
GAGCTGAAAG AAGCCACACC TTCTCCAGTG AGTGAATCAC ACAGTGAAGA GACTGTACCC 
AGTGACAGTG GCATTGGAAC AGACAATAAT AGCACCTCTG ACCGTGGGGA AAAAGCTGGA 
AGTGCCAATG GCTTACCTGG TGTGGGGGTC TCACAGGGTA TGGCAGGTGG ACTCTTAATG 
CCTGGAGTCA TAGGTCCAGG TATTGGAACG GGAATTGGAC TTTCTTCGAG AGGTCGGAGG 
AGGCACTCAT CTTCAGTTCT TCTAGAACGT CCCTCACCTA CACCCTCACC TCTTGGAGTG 
AGAGCATCAC CTGACCCAAG AAGATCGCAC CCTGCTGCCC CATCTTCTGC TTTGGTTGGA 
CATAAAGAGA AACACAAGCA TAAGTGCAAG CGTCGTGGGC ATGGCTGCCC TAGCTATGAC 
AAACTAAAAA GGCAAAAGCG TAAACGCAAG AAGAAGTACC TGCAGCTACG GTCCCGCAGA 
CAAGACCCAG ATTTTTTGGC TGATTTAGAT GAGATATGTG TGCAGCTTAG TGATATACGA 
ATTTCTCATC GAACACCTAC ACTTCGTTTG GGCAGCAGCA TAGGTTTGGG TGCCGGGGCT 
GGTCCTGGCC GTGGGTCTGG CTTGGGAAGT ACCAGACCTA GCAGCAGCCT TGGAGGCGGA 
ACCAATCCAC CACCCCATCA CTATCTGCAT AGAGATCTAT TACCCACAAT ATTCAGGATA 
AATTTTAGTG GGTATTATTC ACCCCACCCA GCCTACCCAT GTGATTCACT TCATTACGTT 
CGTAAGCCAG ATCTTAAAAA GAAAAGAGGT CGACCACCTA AGCTTCGGGA ATCCATATCA 
GAAGTACCTT TTGTACCTGG TTTAGGCTTT CCCCTCTCAA GTGGAGGTTT CTATCACCCT 
TCTTATGGTG TCCCATACTC TTCTACCCCA CTGGGACTTG GTTACTATCG TGGTTATACC 
CCACCAAGTG CCCTGTACCC CCATCCTCAT CACCAGGCTC CCCACTCTGG CCCATCCCAC 
CATGTTCATT CACCATCTTT TCCTCCACCT CCACCCCCTT CTTATGTGCA TCATCATCAC 
CCATCCCACC TGCTTCTTAA CCCCTCCAAA TTTCACAAGA AGAAACATAA GCTACTTAGG 
CAAGAGTATT TAGGTGGTGG TCGTTCATCC ATGCTCTATC CTGCCATGTC ATCTGAGCTG 
TCATTTGGCT GGCACCACAA ACACAAGCAT AGGCACAAAC ACCGTGATCG CTGTGGAGAA 
GAGGAGGGTG ATGATGGTGG TGGAGTTTTA GGTGGTATAG GAGGCAGAGG TGTTGGAGGT 
GAGGGCTTGG GGGCCGGAAG AGGGGAGAGA GGGAGCGTAG AGTCCCTGCA GCACTGCCGG 
TTTGGACGAG ATGGCTCTGC TGACTCCCGA ACTAACAAAC AGTCAGCTTC TGCCAACTCC 
CCTTCCTCTT CCTCCACATC CTCTGCAGAA CGGTACAAGC GAAAAGAGGC CTCTATGTCT 
TGTCTTGGGC CCTCACGACT GTCTTTGGGC ACTGCAAGTC GGGGACATCA TCCTGTTGAC 
TCCTGGTTCA GAATGGGAAG TTCTGATACT GATTACTCCA AGCTCTCTAG AAACCAGTCT 
TTTTCGGGGT CTGGTGCATT CTCTGAAGGA CATGTGCAAA ATCCAGTGGA TTGCTCAGAG 
AGTGATGAGG AAGATGCTGC TTCCCCCATT GAAGAGCCAG AGATCAGCCA TCACACAAAC 
CTGTTTGCAT CTGCACTATC AAGGACCTCC CTCAAAGGAA GTCGCAGCAA AAAAGGTGGA 
CCATTAGAGA GTCCCAGTCT CTCACGGTTG GACAGGACGG TTAGAAGGGA TCGTTCAACA 
TCTTCAGAGA AAAGAGAAAT TGGGAATGTA TCTAGCTTGC AGAACAGAGT TGGCCAATCC 
AGTGTTCCTG ATGTTTCTGA GGGTCTCCAT CATCGGCACC ACCATCACCA CCACCCTCCT 
CTATCCCTCT CGCACGCTGC CTCTTGCCTG CCGCACTGCA CTCTAGACCA GCCTGGAAAC 
ACTGCACCAC TCCGGCCCTC CAGCCGCCAA GCTAGTCCAT CCACATGCTC CTCCCAGCCT 
GCATGCTGCC TTGATTCCCC ACCTTCACAT CTCTCCCACC GGCCCCCTCC CAAAAACAAC 
CTGCTTCATG TCAACAAGAT CCTCCGTGCA AAGAAGCTCC AAAGGCAAGC ACGTACAGGG 
AACAACATGG TGAAAAAACG GGGTCCAGGA CGTCCCCGCA AGAACCCGCT GCCCTCACCT 
CCTGCAACAC CCCCACCACA GACTTCCGAA CAAGACAACC ACCCATTACC TGAAAGGCAC 
CGAGCAACTG CACTCTGGGA GGCAGATACT GTAGTTGATG CCATTGAGGC TGTCGTACAG 
AACCAGCGCA AAAAGGGACG CAAGCGGAAG CACTGGGAAA GGGAGACTGA AGGAGGTGAT 
GAAGAAGAGG AGGATGCGGA GGAAGAAAGA CAGGTGGAGG ACGAAGAAGA AGAAGAAGAC 
GAAGAACAAG AACAACAGTC AGCTGCTAGA GAAGACGATG TAATGTCGAG AGTTAGAACA 
GAAGGAAGCA GGCAATGGTT GACCCAGGAA GAAGTGCACT GTTTTCGCAG TGCACTGGAG 
GGAAAACCTG ATGGCTCCCC TGAGCGCCCA GGCCCTGCTA CTTCGGAACC AACCCCAAAC 
CCATCAGTGA CCAGCCAACG TGAGAAGAGA GTTGCTAGAC CACCCAAAAA GAAGTTTCAA 
AAGGCGGGAC TTTACTCTGA TGTGTACAAG ACTGATGATC CTCGCAGTCA GCTGCTACAG 
TTAAAGAAAG AGAAGCTAGA ATACACTCCA GGTGAACATG ACTATGGCCT TCTTCCTGCT 
CCCATTCATG TTGGGAAATA CTTGAGACAG AAACGCATTG ATTTCCAGCT GCCTTACGAC 
ATCCTGTGGC TTTGGAAACA TGATCAGCTC TACAAGAGAC CTGACGTGCC TCTCTACAAG 
AAGATCAGAT CCAATGTCTA TGTGGATGTG AAGCCTCTTT CTGGCTATGA AGCCACTACC 
TGTAACTGCA GGTTGCCTGA CGACTCTAGT GAAAAGGGCT GTCAAGATGA TTGTCTAAAC 
AGGATGATTT ATGCCGAGTG CTCTCCAAGT ACGTGTCCGT GTTCCGATCA GTGCGATAAC 
CAGCGGATCC AGAAGCATGA GTGGGTTCAG TGCCTCGAGC GCTTCCGAGC TGAGGGAAAG 
GGCTGGGGCA TCCGGACCAA GCAGCCCCTT CGTGCCGGCC AATTTATCAT CGAGTACTTG 
GGAGAGGTGG TCAGCGAACA AGAGTTCAGA AAATACATTG CGTCTGACTG TAGGTTGCAC 
CAAGTCAAAA TTCCAATGTT GGGGGAAAAT AAAAAGCAGA TTGGTGTGAG TCGCATGATG 
GAGCAGTACT TCTCTCACAG CGGCCACTAC TGCCTGAATC TGGACAGCGG CATGGTCATC 
GATAGCTACC GCATGGGCAA CGAGGCTCGC TTTGTCAACC ACAGCTGCGA GCCCAACTGT 
GAGATGCAGA AATGGTCGGT GAACGGAGTG TACCGAATTG GCCTGTTTGC TCTAAAAGAC 
ATCAACAGCG GAACGGAACT GACATATGAC TACAACTTCC ACTCCTTCAA CACAGAGGAA 
CAACAAGTCT GCAAGTGTGG CTCAGAGGGT TGCCGAGGCA TCATTGGCGG CAAGAGCAAG 
CGAATCAATG GGCTGCCTGC TAAAGGGGCC GGATCTGGAG GCGGAGCCCG GAGACTTGGA 
AGATTGAAAG AGAAACGCAA GTCCAAACAC TCACTGAAGA AGCGGGAGGA AGAGTCCAGT 
GACAGCAGCA AGTTTTACCA GCACCTTTTA ATGAAGCCCA TGTCAAACAG AGAGAGGAAC 
TTTGTGCTGA AACATCGGGT CTTTCTGCTG CGGAATTGGG AGAAGATGCG GGAGAAACAG 
GAGCTTCTGA AGCGTGAGGG AGAGCGGGAG AGGGAGAACA GCGGTCTGTC TCTGTACACC 
CGCTGGGGAG GAGTCATCCG GGATGATGGA AACATCAAAT CAGATGTATT TCTGACTCAG 
TTCTCAGCGC TGCAGACGTC CCGCTCTGTA CGCACACGCA GACTTGCGGC AGCCGAGGAG 
AACACTGAAG TGACACGCAC CGCACGTCTG GCACACATTT TCAAAGAGAT CTGCGATATG 
ATCACTAGCT ACAAGGATTC ATCTGGTCAG CCTCTTGCTG CTCCGCTGCT TAACCTGCCT 
TCTAGGAAGA GGAACACACA ATACTATGAG AAGGTCACCG ATCCTCTAGA TCTCAGCACC 
ATAGATAAGC AGATCCTCAG CGGACACTAC AAAACCGATG AGGCGTTTGA TGCGGACATG 
CTCAAAGTGT TTCGCAATGC TGAGAAGTAC TATGGGCGTA AGTCTGCTGT GGGCCGTGAC 
GTGTGCCGGC TGAGGAAGGC TTATTATGGT GCCCGTCATG AGGCTGCTGT TCAGATCGAT 
GAGATTGTGG GTGAGACGGC AAGTGAAGCG GACAGCTCTG ATTCGCTGGA GAGAGACCAC 
GCCCACCACC ATCACCATGA CGGAGGGGGC AGCCATGATA AGGATGATGA TGTGATCCGC 
TGCATCTGTG GCATGTACAA GGATGAGGGA CTCATGATTC AGTGCGAGAA GTGCATGGTG 
TGGCAGCACT GTGATTGTAT GCGTCTGGAG GCGGATGTGG AGCATTATCT CTGTGAACAG 
TGTGACCCTC GTCCAGTGGA CAGAGAGGTT CCAATGGTGC CTCAACCCAG CTATGCCCAA 
TCAGGATTCA TCTATTACAT ATGCCTGCTT CGCGATGACT TACTGTTGCA TCAGGGCGAT 
TGTGTGTACC TCATGCGGGA CAGCAGACGC ACAACAGAAG GTCAGCCGGT GCGTCAGTCA 
TACCGCCTCC TGTCTCACAT CAACAGAGAC AAACTGGACA TCTTCCGTAT CGAGAAACTC 
TGGAAGAATG AAAAAGGTGA GAGGTTTGCA TTTGGTCATC ATTACTTCCG TCCTCATGAG 
ACGCATCACT CCCCTTCCCG CCGCTTCTAC CACAACGAGC TGTTTCGAGT GCCTCTGTAT 
GAGATCATCC CGCTGGAGGC CGTGGTGGGC ACCTGCTGCG TGCTGGACCT CTACACATAC 
TGCAAGGGTC GTCCAAAAGG GGTAAAGGAG CAGGACGTTT ACATCTGCGA CTACCGGCTG 
GATAAATCCG CACACCTGTT CTACAAGATC CACCGCAACC GTTACCCAGT GTGCACCAAG 
CCCTATGCGT TCAACCACTT CCCCAAGAGA CTGACGCCCA AGAGGGACTT CTCTCCTCAC 
TATGTTCCAG ATAACTACAA GAGGAACGGC GGACGCTCTG CTTGGAAGAG CGAGCGGCCC 
AAAGATGAAG AACCATCATC CTCCAGCACG TCCTGCGAGC GTCTGTCCTC CAGCTTCCCT 
AGAGAAGGAG ACGAGGACGG AGGACGAGGA GCAGAAGGAG ACGCGGAGGC CGCTCAAGAG 
GGATCCAGCA CTCCGAGACC TCAGAGATCC TCGCCACTGG AGAGCAGGAC GGCCGGAGAG 
GAGGATGAGG CGGAGGAGGA AGAGGACAGA GAGAGGAGGA GAGAGATGCA GGAGGAGGAG 
GAGGAGGAGG GATGCAGAGA GAAAGCAGGA GCGCAGGAGC CCATAGACGC ACAGTGCTCC 
TCCTCCATCT CCTCTTCTCC TCTTCATCCC GCCATTCTGG GCAGGAGAGA AGCGCAGAGA 
GAGCGGCTCA ACAAGATCCT GCTCGACCTG CTGCACCGCG CGCCCACTAA GAACGCGATA 
GATGTGACGT ATCTTCTTGA GGAAGGCTCG GGTCGTCGCC TTCGCAGACG GACGCTTGGA 
CTGAGCGAAT TCATTTGCAG AAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159540.1
CDS	465..9431
Translation	MDQRTQGGPSTPPPLPANAPPAEREKEGVGGKKDDEEEKKKREKLRDGAQNEASGAESGTGDQPQQPTQFSVKETSYSEGNVKLKIGLQAKRMKKPPKILENYVCRPAFRATVRHSGGRGGGRANRGAGASDANSNSTSTSNGKEGEKKPSVNRGASQSAAAPAASTPSPSLPPASINTVTPVNGNAPAKRGPPKLACKSEGKADVKSVASSEKPLNLHRPTTESKSHSSGKKNHVPQTNSAPAPSPPAVPPAATSQQDPKIDGILKTPQCNFLEGQKEQERGISSWGTPTVTEKLAQLIATCPPSKCPKPKARKVSPAPAHSSSPPLISNLQRPERAMANRNTYSRAVHLAQPPPVSRPPGRPYGSKNKDSILEKFSGTSKKEESDGLSKGVSGSSSTTTTTTSSALSQNSSRLTTTTSTNESTDINNTDGMKSQSRLGHCPSHSSSAAASPPFSSSSSVERIGSTSCNIGLPGSLTSRPKASPSRIEDFSEKDKERDREKDTDSPRDLSKGITPAITGKQDAKERCSLSAPRNDRGKSSSPVKRSPNQESRVSGVPSPAESTKCTISPSYPSGASRSPPPLCDDTGAISPASPSEQDSKPLKKRRGRRPRWTKAVSRTHKSLHESPSNHQQIPTPMPSGLSFSSPIRESVEQPLSDSYTSPEDSDIFDSSPKKRGRPKTKMPRLDAPAHGCTPNKLAPSKSFSSLLKSKEEQDPPVLHPEVDFNPPKMMARKRGRPKRSPPTLPQETQPPTLAPESGEASVGEKPFRNKGNGQLIMKTIIGKINKMKTVKRKRLLSQILLGPKPAEEQECSRSSGPTGSPVATKPQSLTSLAATFGGKLGPQINVSKKGTIYMGKRRGRKPKTSTNPSPGAPTPPPEPFLSPNTSSPHHHQVQALQNQPHQLPPSEVFPSPSLSQSSGGQSPISDASFVEPGSVHFSSHPHCSHSHQVHHSFSFPPPTFSAPSARSVASAATSTAHPSQKKSSCRGYHHHHHHYRQHYHYRKFSPPRPLLPTSPAPLSELKEATPSPVSESHSEETVPSDSGIGTDNNSTSDRGEKAGSANGLPGVGVSQGMAGGLLMPGVIGPGIGTGIGLSSRGRRRHSSSVLLERPSPTPSPLGVRASPDPRRSHPAAPSSALVGHKEKHKHKCKRRGHGCPSYDKLKRQKRKRKKKYLQLRSRRQDPDFLADLDEICVQLSDIRISHRTPTLRLGSSIGLGAGAGPGRGSGLGSTRPSSSLGGGTNPPPHHYLHRDLLPTIFRINFSGYYSPHPAYPCDSLHYVRKPDLKKKRGRPPKLRESISEVPFVPGLGFPLSSGGFYHPSYGVPYSSTPLGLGYYRGYTPPSALYPHPHHQAPHSGPSHHVHSPSFPPPPPPSYVHHHHPSHLLLNPSKFHKKKHKLLRQEYLGGGRSSMLYPAMSSELSFGWHHKHKHRHKHRDRCGEEEGDDGGGVLGGIGGRGVGGEGLGAGRGERGSVESLQHCRFGRDGSADSRTNKQSASANSPSSSSTSSAERYKRKEASMSCLGPSRLSLGTASRGHHPVDSWFRMGSSDTDYSKLSRNQSFSGSGAFSEGHVQNPVDCSESDEEDAASPIEEPEISHHTNLFASALSRTSLKGSRSKKGGPLESPSLSRLDRTVRRDRSTSSEKREIGNVSSLQNRVGQSSVPDVSEGLHHRHHHHHHPPLSLSHAASCLPHCTLDQPGNTAPLRPSSRQASPSTCSSQPACCLDSPPSHLSHRPPPKNNLLHVNKILRAKKLQRQARTGNNMVKKRGPGRPRKNPLPSPPATPPPQTSEQDNHPLPERHRATALWEADTVVDAIEAVVQNQRKKGRKRKHWERETEGGDEEEEDAEEERQVEDEEEEEDEEQEQQSAAREDDVMSRVRTEGSRQWLTQEEVHCFRSALEGKPDGSPERPGPATSEPTPNPSVTSQREKRVARPPKKKFQKAGLYSDVYKTDDPRSQLLQLKKEKLEYTPGEHDYGLLPAPIHVGKYLRQKRIDFQLPYDILWLWKHDQLYKRPDVPLYKKIRSNVYVDVKPLSGYEATTCNCRLPDDSSEKGCQDDCLNRMIYAECSPSTCPCSDQCDNQRIQKHEWVQCLERFRAEGKGWGIRTKQPLRAGQFIIEYLGEVVSEQEFRKYIASDCRLHQVKIPMLGENKKQIGVSRMMEQYFSHSGHYCLNLDSGMVIDSYRMGNEARFVNHSCEPNCEMQKWSVNGVYRIGLFALKDINSGTELTYDYNFHSFNTEEQQVCKCGSEGCRGIIGGKSKRINGLPAKGAGSGGGARRLGRLKEKRKSKHSLKKREEESSDSSKFYQHLLMKPMSNRERNFVLKHRVFLLRNWEKMREKQELLKREGERERENSGLSLYTRWGGVIRDDGNIKSDVFLTQFSALQTSRSVRTRRLAAAEENTEVTRTARLAHIFKEICDMITSYKDSSGQPLAAPLLNLPSRKRNTQYYEKVTDPLDLSTIDKQILSGHYKTDEAFDADMLKVFRNAEKYYGRKSAVGRDVCRLRKAYYGARHEAAVQIDEIVGETASEADSSDSLERDHAHHHHHDGGGSHDKDDDVIRCICGMYKDEGLMIQCEKCMVWQHCDCMRLEADVEHYLCEQCDPRPVDREVPMVPQPSYAQSGFIYYICLLRDDLLLHQGDCVYLMRDSRRTTEGQPVRQSYRLLSHINRDKLDIFRIEKLWKNEKGERFAFGHHYFRPHETHHSPSRRFYHNELFRVPLYEIIPLEAVVGTCCVLDLYTYCKGRPKGVKEQDVYICDYRLDKSAHLFYKIHRNRYPVCTKPYAFNHFPKRLTPKRDFSPHYVPDNYKRNGGRSAWKSERPKDEEPSSSSTSCERLSSSFPREGDEDGGRGAEGDAEAAQEGSSTPRPQRSSPLESRTAGEEDEAEEEEDRERRREMQEEEEEEGCREKAGAQEPIDAQCSSSISSSPLHPAILGRREAQRERLNKILLDLLHRAPTKNAIDVTYLLEEGSGRRLRRRTLGLSEFICRK

Target ORF information:

RefSeq Version	XM_005159483.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ash1 (absent, small, or homeotic)-like (Drosophila) (ash1l), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159483.3

ORF Insert Sequence:

ATGGATCAGA GGACACAGGG GGGGCCTTCT ACGCCGCCCC CACTCCCTGC CAATGCCCCC 
CCAGCAGAAC GTGAAAAAGA AGGTGTAGGA GGAAAGAAGG ACGATGAAGA AGAGAAAAAG 
AAACGGGAGA AACTAAGAGA TGGGGCACAG AATGAAGCTA GTGGTGCTGA AAGTGGGACT 
GGTGACCAGC CGCAACAACC CACGCAGTTT TCGGTCAAGG AGACCAGCTA CTCTGAGGGT 
AACGTTAAGC TCAAAATAGG CCTGCAGGCC AAACGCATGA AGAAACCTCC TAAGATCCTG 
GAGAACTATG TCTGCCGACC AGCCTTCCGT GCCACTGTAA GGCATAGTGG AGGTAGAGGC 
GGAGGACGTG CTAATCGTGG GGCTGGAGCC AGTGATGCGA ACAGCAACTC TACTAGCACA 
AGTAATGGAA AGGAAGGGGA GAAAAAGCCC TCTGTCAACC GAGGAGCATC TCAGTCAGCT 
GCTGCTCCTG CTGCTTCTAC TCCCTCACCC TCTCTTCCTC CTGCTTCCAT CAACACTGTC 
ACTCCAGTGA ATGGAAATGC TCCAGCAAAA AGGGGTCCTC CAAAACTTGC CTGCAAATCA 
GAGGGAAAGG CTGACGTAAA ATCTGTTGCT TCATCAGAGA AACCTCTTAA TCTCCATCGC 
CCCACGACAG AAAGTAAATC ACACTCCTCT GGAAAGAAAA ACCATGTTCC ACAAACAAAC 
TCTGCCCCTG CCCCATCTCC ACCAGCAGTG CCCCCAGCTG CCACTTCGCA ACAGGACCCT 
AAAATAGATG GAATCTTAAA AACGCCTCAA TGCAATTTTT TGGAGGGGCA GAAGGAACAA 
GAAAGAGGGA TTTCTTCTTG GGGTACCCCT ACAGTAACTG AAAAACTTGC TCAGCTCATA 
GCTACATGCC CACCTTCAAA GTGCCCTAAG CCAAAAGCCC GAAAGGTATC GCCAGCTCCA 
GCACACAGTA GCTCACCTCC GTTAATTTCT AATCTGCAGC GCCCTGAACG GGCAATGGCT 
AATAGAAACA CCTATTCCAG GGCGGTTCAC CTCGCCCAAC CACCACCTGT ATCACGGCCC 
CCTGGTCGCC CATATGGGTC TAAAAACAAA GACAGTATCC TTGAGAAATT TTCTGGAACT 
TCTAAAAAAG AGGAATCAGA TGGACTTTCA AAAGGGGTCT CCGGTAGCAG CAGCACAACC 
ACCACCACCA CTAGTTCAGC ACTGTCTCAA AACAGCAGTC GCTTGACGAC AACAACATCC 
ACAAATGAAA GCACTGACAT TAACAACACA GATGGAATGA AATCTCAGTC TCGTCTTGGG 
CATTGCCCAT CTCATTCTTC ATCCGCAGCT GCTTCACCTC CATTTTCTTC TTCATCCTCC 
GTAGAGAGAA TAGGAAGTAC AAGCTGTAAC ATAGGTTTGC CAGGCTCTCT AACCTCACGC 
CCTAAAGCAA GCCCCTCTCG AATAGAGGAT TTTAGTGAGA AAGACAAAGA ACGTGACCGG 
GAAAAGGACA CAGACAGTCC AAGAGATTTA AGTAAAGGTA TCACTCCAGC TATAACTGGG 
AAGCAGGATG CAAAAGAAAG GTGTTCTTTA TCAGCTCCTC GAAATGACAG GGGTAAGAGC 
TCAAGCCCAG TCAAACGTAG TCCCAATCAG GAAAGTCGCG TTAGTGGGGT ACCAAGTCCT 
GCTGAGTCCA CCAAATGCAC TATTTCTCCT TCATACCCTT CTGGTGCCAG CAGGAGCCCA 
CCACCACTAT GCGATGACAC CGGGGCTATT TCTCCTGCAT CACCATCTGA GCAGGATTCA 
AAACCTCTTA AGAAGCGACG AGGACGACGA CCTCGCTGGA CGAAGGCAGT GAGTCGCACC 
CACAAGTCTT TACATGAGTC TCCTTCTAAC CATCAGCAAA TCCCCACACC AATGCCCTCA 
GGGCTTTCTT TCTCATCCCC TATTCGGGAG TCTGTGGAGC AGCCTCTCAG TGACAGTTAC 
ACATCCCCAG AGGATTCTGA TATTTTCGAT TCTTCGCCCA AGAAGAGAGG ACGTCCAAAG 
ACCAAAATGC CTCGATTAGA TGCACCAGCA CATGGCTGCA CCCCAAACAA GTTGGCACCT 
TCAAAATCCT TCTCCAGTCT GCTCAAGTCT AAGGAAGAGC AGGACCCCCC TGTTTTGCAC 
CCTGAAGTGG ATTTTAATCC ACCTAAAATG ATGGCAAGAA AGCGAGGGCG ACCCAAGCGC 
TCACCTCCAA CTCTTCCTCA AGAAACCCAG CCTCCGACTC TTGCTCCTGA GTCAGGGGAA 
GCATCAGTTG GGGAAAAACC TTTTCGTAAT AAGGGTAATG GGCAGCTCAT TATGAAGACG 
ATCATAGGCA AAATCAATAA AATGAAGACA GTAAAACGGA AGAGGCTCCT CAGCCAAATA 
CTGCTTGGGC CAAAACCAGC AGAGGAGCAG GAATGCTCAA GAAGCAGTGG GCCAACAGGG 
TCTCCGGTTG CTACAAAACC ACAGTCCCTG ACATCACTTG CTGCTACATT TGGTGGCAAA 
TTAGGGCCCC AGATTAATGT AAGCAAAAAG GGGACAATAT ATATGGGTAA AAGAAGGGGT 
CGTAAGCCAA AAACATCAAC TAATCCTTCC CCAGGTGCCC CCACACCCCC TCCTGAGCCT 
TTCTTATCAC CAAACACTTC ATCCCCTCAT CATCACCAGG TGCAGGCTTT ACAAAACCAA 
CCACACCAGT TGCCTCCATC TGAGGTGTTT CCTTCCCCAT CACTATCACA GTCAAGTGGG 
GGCCAAAGCC CCATTAGTGA TGCTAGCTTT GTAGAGCCTG GCTCAGTGCA TTTCTCATCT 
CACCCACACT GCAGTCATTC TCATCAGGTC CACCACTCAT TCTCATTTCC ACCGCCTACT 
TTTTCAGCCC CCTCTGCTCG ATCTGTAGCA AGTGCTGCAA CATCCACTGC ACACCCATCT 
CAGAAGAAGT CCTCTTGCCG GGGTTACCAT CATCACCACC ACCATTACCG GCAGCACTAT 
CATTATCGAA AATTTTCTCC TCCAAGGCCA CTATTGCCAA CGTCTCCAGC TCCACTGAGT 
GAGCTGAAAG AAGCCACACC TTCTCCAGTG AGTGAATCAC ACAGTGAAGA GACTGTACCC 
AGTGACAGTG GCATTGGAAC AGACAATAAT AGCACCTCTG ACCGTGGGGA AAAAGCTGGA 
AGTGCCAATG GCTTACCTGG TGTGGGGGTC TCACAGGGTA TGGCAGGTGG ACTCTTAATG 
CCTGGAGTCA TAGGTCCAGG TATTGGAACG GGAATTGGAC TTTCTTCGAG AGGTCGGAGG 
AGGCACTCAT CTTCAGTTCT TCTAGAACGT CCCTCACCTA CACCCTCACC TCTTGGAGTG 
AGAGCATCAC CTGACCCAAG AAGATCGCAC CCTGCTGCCC CATCTTCTGC TTTGGTTGGA 
CATAAAGAGA AACACAAGCA TAAGTGCAAG CGTCGTGGGC ATGGCTGCCC TAGCTATGAC 
AAACTAAAAA GGCAAAAGCG TAAACGCAAG AAGAAGTACC TGCAGCTACG GTCCCGCAGA 
CAAGACCCAG ATTTTTTGGC TGATTTAGAT GAGATATGTG TGCAGCTTAG TGATATACGA 
ATTTCTCATC GAACACCTAC ACTTCGTTTG GGCAGCAGCA TAGGTTTGGG TGCCGGGGCT 
GGTCCTGGCC GTGGGTCTGG CTTGGGAAGT ACCAGACCTA GCAGCAGCCT TGGAGGCGGA 
ACCAATCCAC CACCCCATCA CTATCTGCAT AGAGATCTAT TACCCACAAT ATTCAGGATA 
AATTTTAGTG GGTATTATTC ACCCCACCCA GCCTACCCAT GTGATTCACT TCATTACGTT 
CGTAAGCCAG ATCTTAAAAA GAAAAGAGGT CGACCACCTA AGCTTCGGGA ATCCATATCA 
GAAGTACCTT TTGTACCTGG TTTAGGCTTT CCCCTCTCAA GTGGAGGTTT CTATCACCCT 
TCTTATGGTG TCCCATACTC TTCTACCCCA CTGGGACTTG GTTACTATCG TGGTTATACC 
CCACCAAGTG CCCTGTACCC CCATCCTCAT CACCAGGCTC CCCACTCTGG CCCATCCCAC 
CATGTTCATT CACCATCTTT TCCTCCACCT CCACCCCCTT CTTATGTGCA TCATCATCAC 
CCATCCCACC TGCTTCTTAA CCCCTCCAAA TTTCACAAGA AGAAACATAA GCTACTTAGG 
CAAGAGTATT TAGGTGGTGG TCGTTCATCC ATGCTCTATC CTGCCATGTC ATCTGAGCTG 
TCATTTGGCT GGCACCACAA ACACAAGCAT AGGCACAAAC ACCGTGATCG CTGTGGAGAA 
GAGGAGGGTG ATGATGGTGG TGGAGTTTTA GGTGGTATAG GAGGCAGAGG TGTTGGAGGT 
GAGGGCTTGG GGGCCGGAAG AGGGGAGAGA GGGAGCGTAG AGTCCCTGCA GCACTGCCGG 
TTTGGACGAG ATGGCTCTGC TGACTCCCGA ACTAACAAAC AGTCAGCTTC TGCCAACTCC 
CCTTCCTCTT CCTCCACATC CTCTGCAGAA CGGTACAAGC GAAAAGAGGC CTCTATGTCT 
TGTCTTGGGC CCTCACGACT GTCTTTGGGC ACTGCAAGTC GGGGACATCA TCCTGTTGAC 
TCCTGGTTCA GAATGGGAAG TTCTGATACT GATTACTCCA AGCTCTCTAG AAACCAGTCT 
TTTTCGGGGT CTGGTGCATT CTCTGAAGGA CATGTGCAAA ATCCAGTGGA TTGCTCAGAG 
AGTGATGAGG AAGATGCTGC TTCCCCCATT GAAGAGCCAG AGATCAGCCA TCACACAAAC 
CTGTTTGCAT CTGCACTATC AAGGACCTCC CTCAAAGGAA GTCGCAGCAA AAAAGGTGGA 
CCATTAGAGA GTCCCAGTCT CTCACGGTTG GACAGGACGG TTAGAAGGGA TCGTTCAACA 
TCTTCAGAGA AAAGAGAAAT TGGGAATGTA TCTAGCTTGC AGAACAGAGT TGGCCAATCC 
AGTGTTCCTG ATGTTTCTGA GGGTCTCCAT CATCGGCACC ACCATCACCA CCACCCTCCT 
CTATCCCTCT CGCACGCTGC CTCTTGCCTG CCGCACTGCA CTCTAGACCA GCCTGGAAAC 
ACTGCACCAC TCCGGCCCTC CAGCCGCCAA GCTAGTCCAT CCACATGCTC CTCCCAGCCT 
GCATGCTGCC TTGATTCCCC ACCTTCACAT CTCTCCCACC GGCCCCCTCC CAAAAACAAC 
CTGCTTCATG TCAACAAGAT CCTCCGTGCA AAGAAGCTCC AAAGGCAAGC ACGTACAGGG 
AACAACATGG TGAAAAAACG GGGTCCAGGA CGTCCCCGCA AGAACCCGCT GCCCTCACCT 
CCTGCAACAC CCCCACCACA GACTTCCGAA CAAGACAACC ACCCATTACC TGAAAGGCAC 
CGAGCAACTG CACTCTGGGA GGCAGATACT GTAGTTGATG CCATTGAGGC TGTCGTACAG 
AACCAGCGCA AAAAGGGACG CAAGCGGAAG CACTGGGAAA GGGAGACTGA AGGAGGTGAT 
GAAGAAGAGG AGGATGCGGA GGAAGAAAGA CAGGTGGAGG ACGAAGAAGA AGAAGAAGAC 
GAAGAACAAG AACAACAGTC AGCTGCTAGA GAAGACGATG TAATGTCGAG AGTTAGAACA 
GAAGGAAGCA GGCAATGGTT GACCCAGGAA GAAGTGCACT GTTTTCGCAG TGCACTGGAG 
GGAAAACCTG ATGGCTCCCC TGAGCGCCCA GGCCCTGCTA CTTCGGAACC AACCCCAAAC 
CCATCAGTGA CCAGCCAACG TGAGAAGAGA GTTGCTAGAC CACCCAAAAA GAAGTTTCAA 
AAGGCGGGAC TTTACTCTGA TGTGTACAAG ACTGATGATC CTCGCAGTCA GCTGCTACAG 
TTAAAGAAAG AGAAGCTAGA ATACACTCCA GGTGAACATG ACTATGGCCT TCTTCCTGCT 
CCCATTCATG TTGGGAAATA CTTGAGACAG AAACGCATTG ATTTCCAGCT GCCTTACGAC 
ATCCTGTGGC TTTGGAAACA TGATCAGCTC TACAAGAGAC CTGACGTGCC TCTCTACAAG 
AAGATCAGAT CCAATGTCTA TGTGGATGTG AAGCCTCTTT CTGGCTATGA AGCCACTACC 
TGTAACTGCA GGTTGCCTGA CGACTCTAGT GAAAAGGGCT GTCAAGATGA TTGTCTAAAC 
AGGATGATTT ATGCCGAGTG CTCTCCAAGT ACGTGTCCGT GTTCCGATCA GTGCGATAAC 
CAGCGGATCC AGAAGCATGA GTGGGTTCAG TGCCTCGAGC GCTTCCGAGC TGAGGGAAAG 
GGCTGGGGCA TCCGGACCAA GCAGCCCCTT CGTGCCGGCC AATTTATCAT CGAGTACTTG 
GGAGAGGTGG TCAGCGAACA AGAGTTCAGA AAATACATTG CGTCTGACTG TAGGTTGCAC 
CAAGTCAAAA TTCCAATGTT GGGGGAAAAT AAAAAGCAGA TTGGTGTGAG TCGCATGATG 
GAGCAGTACT TCTCTCACAG CGGCCACTAC TGCCTGAATC TGGACAGCGG CATGGTCATC 
GATAGCTACC GCATGGGCAA CGAGGCTCGC TTTGTCAACC ACAGCTGCGA GCCCAACTGT 
GAGATGCAGA AATGGTCGGT GAACGGAGTG TACCGAATTG GCCTGTTTGC TCTAAAAGAC 
ATCAACAGCG GAACGGAACT GACATATGAC TACAACTTCC ACTCCTTCAA CACAGAGGAA 
CAACAAGTCT GCAAGTGTGG CTCAGAGGGT TGCCGAGGCA TCATTGGCGG CAAGAGCAAG 
CGAATCAATG GGCTGCCTGC TAAAGGGGCC GGATCTGGAG GCGGAGCCCG GAGACTTGGA 
AGATTGAAAG AGAAACGCAA GTCCAAACAC TCACTGAAGA AGCGGGAGGA AGAGTCCAGT 
GACAGCAGCA AGTTTTACCA GCACCTTTTA ATGAAGCCCA TGTCAAACAG AGAGAGGAAC 
TTTGTGCTGA AACATCGGGT CTTTCTGCTG CGGAATTGGG AGAAGATGCG GGAGAAACAG 
GAGCTTCTGA AGCGTGAGGG AGAGCGGGAG AGGGAGAACA GCGGTCTGTC TCTGTACACC 
CGCTGGGGAG GAGTCATCCG GGATGATGGA AACATCAAAT CAGATGTATT TCTGACTCAG 
TTCTCAGCGC TGCAGACGTC CCGCTCTGTA CGCACACGCA GACTTGCGGC AGCCGAGGAG 
AACACTGAAG TGACACGCAC CGCACGTCTG GCACACATTT TCAAAGAGAT CTGCGATATG 
ATCACTAGCT ACAAGGATTC ATCTGGTCAG CCTCTTGCTG CTCCGCTGCT TAACCTGCCT 
TCTAGGAAGA GGAACACACA ATACTATGAG AAGGTCACCG ATCCTCTAGA TCTCAGCACC 
ATAGATAAGC AGATCCTCAG CGGACACTAC AAAACCGATG AGGCGTTTGA TGCGGACATG 
CTCAAAGTGT TTCGCAATGC TGAGAAGTAC TATGGGCGTA AGTCTGCTGT GGGCCGTGAC 
GTGTGCCGGC TGAGGAAGGC TTATTATGGT GCCCGTCATG AGGCTGCTGT TCAGATCGAT 
GAGATTGTGG GTGAGACGGC AAGTGAAGCG GACAGCTCTG ATTCGCTGGA GAGAGACCAC 
GCCCACCACC ATCACCATGA CGGAGGGGGC AGCCATGATA AGGATGATGA TGTGATCCGC 
TGCATCTGTG GCATGTACAA GGATGAGGGA CTCATGATTC AGTGCGAGAA GTGCATGGTG 
TGGCAGCACT GTGATTGTAT GCGTCTGGAG GCGGATGTGG AGCATTATCT CTGTGAACAG 
TGTGACCCTC GTCCAGTGGA CAGAGAGGTT CCAATGGTGC CTCAACCCAG CTATGCCCAA 
TCAGGATTCA TCTATTACAT ATGCCTGCTT CGCGATGACT TACTGTTGCA TCAGGGCGAT 
TGTGTGTACC TCATGCGGGA CAGCAGACGC ACAACAGAAG GTCAGCCGGT GCGTCAGTCA 
TACCGCCTCC TGTCTCACAT CAACAGAGAC AAACTGGACA TCTTCCGTAT CGAGAAACTC 
TGGAAGAATG AAAAAGGTGA GAGGTTTGCA TTTGGTCATC ATTACTTCCG TCCTCATGAG 
ACGCATCACT CCCCTTCCCG CCGCTTCTAC CACAACGAGC TGTTTCGAGT GCCTCTGTAT 
GAGATCATCC CGCTGGAGGC CGTGGTGGGC ACCTGCTGCG TGCTGGACCT CTACACATAC 
TGCAAGGGTC GTCCAAAAGG GGTAAAGGAG CAGGACGTTT ACATCTGCGA CTACCGGCTG 
GATAAATCCG CACACCTGTT CTACAAGATC CACCGCAACC GTTACCCAGT GTGCACCAAG 
CCCTATGCGT TCAACCACTT CCCCAAGAGA CTGACGCCCA AGAGGGACTT CTCTCCTCAC 
TATGTTCCAG ATAACTACAA GAGGAACGGC GGACGCTCTG CTTGGAAGAG CGAGCGGCCC 
AAAGATGAAG AACCATCATC CTCCAGCACG TCCTGCGAGC GTCTGTCCTC CAGCTTCCCT 
AGAGAAGGAG ACGAGGACGG AGGACGAGGA GCAGAAGGAG ACGCGGAGGC CGCTCAAGAG 
GGATCCAGCA CTCCGAGACC TCAGAGATCC TCGCCACTGG AGAGCAGGAC GGCCGGAGAG 
GAGGATGAGG CGGAGGAGGA AGAGGACAGA GAGAGGAGGA GAGAGATGCA GGAGGAGGAG 
GAGGAGGAGG GATGCAGAGA GAAAGCAGGA GCGCAGGAGC CCATAGACGC ACAGTGCTCC 
TCCTCCATCT CCTCTTCTCC TCTTCATCCC GCCATTCTGG GCAGGAGAGA AGCGCAGAGA 
GAGCGGCTCA ACAAGATCCT GCTCGACCTG CTGCACCGCG CGCCCACTAA GAACGCGATA 
GATGTGACGT ATCTTCTTGA GGAAGGCTCG GGTCGTCGCC TTCGCAGACG GACGCTTGGA 
CTGAGCGAAT TCATTTGCAG AAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ash1l ( XM_005159484.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159541.1 Danio rerio ash1 (absent, small, or homeotic)-like (Drosophila) (ash1l), transcript variant X2, mRNA.

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CloneID	ODa23935
Clone ID Related Accession (Same CDS sequence)	XM_005159483.3 , XM_005159484.3 , XM_005159484.4 , XM_005159483.4
Accession Version	XM_005159484.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8967bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	histone-lysine N-methyltransferase ASH1L isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159484.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCAGA GGACACAGGG GGGGCCTTCT ACGCCGCCCC CACTCCCTGC CAATGCCCCC 
CCAGCAGAAC GTGAAAAAGA AGGTGTAGGA GGAAAGAAGG ACGATGAAGA AGAGAAAAAG 
AAACGGGAGA AACTAAGAGA TGGGGCACAG AATGAAGCTA GTGGTGCTGA AAGTGGGACT 
GGTGACCAGC CGCAACAACC CACGCAGTTT TCGGTCAAGG AGACCAGCTA CTCTGAGGGT 
AACGTTAAGC TCAAAATAGG CCTGCAGGCC AAACGCATGA AGAAACCTCC TAAGATCCTG 
GAGAACTATG TCTGCCGACC AGCCTTCCGT GCCACTGTAA GGCATAGTGG AGGTAGAGGC 
GGAGGACGTG CTAATCGTGG GGCTGGAGCC AGTGATGCGA ACAGCAACTC TACTAGCACA 
AGTAATGGAA AGGAAGGGGA GAAAAAGCCC TCTGTCAACC GAGGAGCATC TCAGTCAGCT 
GCTGCTCCTG CTGCTTCTAC TCCCTCACCC TCTCTTCCTC CTGCTTCCAT CAACACTGTC 
ACTCCAGTGA ATGGAAATGC TCCAGCAAAA AGGGGTCCTC CAAAACTTGC CTGCAAATCA 
GAGGGAAAGG CTGACGTAAA ATCTGTTGCT TCATCAGAGA AACCTCTTAA TCTCCATCGC 
CCCACGACAG AAAGTAAATC ACACTCCTCT GGAAAGAAAA ACCATGTTCC ACAAACAAAC 
TCTGCCCCTG CCCCATCTCC ACCAGCAGTG CCCCCAGCTG CCACTTCGCA ACAGGACCCT 
AAAATAGATG GAATCTTAAA AACGCCTCAA TGCAATTTTT TGGAGGGGCA GAAGGAACAA 
GAAAGAGGGA TTTCTTCTTG GGGTACCCCT ACAGTAACTG AAAAACTTGC TCAGCTCATA 
GCTACATGCC CACCTTCAAA GTGCCCTAAG CCAAAAGCCC GAAAGGTATC GCCAGCTCCA 
GCACACAGTA GCTCACCTCC GTTAATTTCT AATCTGCAGC GCCCTGAACG GGCAATGGCT 
AATAGAAACA CCTATTCCAG GGCGGTTCAC CTCGCCCAAC CACCACCTGT ATCACGGCCC 
CCTGGTCGCC CATATGGGTC TAAAAACAAA GACAGTATCC TTGAGAAATT TTCTGGAACT 
TCTAAAAAAG AGGAATCAGA TGGACTTTCA AAAGGGGTCT CCGGTAGCAG CAGCACAACC 
ACCACCACCA CTAGTTCAGC ACTGTCTCAA AACAGCAGTC GCTTGACGAC AACAACATCC 
ACAAATGAAA GCACTGACAT TAACAACACA GATGGAATGA AATCTCAGTC TCGTCTTGGG 
CATTGCCCAT CTCATTCTTC ATCCGCAGCT GCTTCACCTC CATTTTCTTC TTCATCCTCC 
GTAGAGAGAA TAGGAAGTAC AAGCTGTAAC ATAGGTTTGC CAGGCTCTCT AACCTCACGC 
CCTAAAGCAA GCCCCTCTCG AATAGAGGAT TTTAGTGAGA AAGACAAAGA ACGTGACCGG 
GAAAAGGACA CAGACAGTCC AAGAGATTTA AGTAAAGGTA TCACTCCAGC TATAACTGGG 
AAGCAGGATG CAAAAGAAAG GTGTTCTTTA TCAGCTCCTC GAAATGACAG GGGTAAGAGC 
TCAAGCCCAG TCAAACGTAG TCCCAATCAG GAAAGTCGCG TTAGTGGGGT ACCAAGTCCT 
GCTGAGTCCA CCAAATGCAC TATTTCTCCT TCATACCCTT CTGGTGCCAG CAGGAGCCCA 
CCACCACTAT GCGATGACAC CGGGGCTATT TCTCCTGCAT CACCATCTGA GCAGGATTCA 
AAACCTCTTA AGAAGCGACG AGGACGACGA CCTCGCTGGA CGAAGGCAGT GAGTCGCACC 
CACAAGTCTT TACATGAGTC TCCTTCTAAC CATCAGCAAA TCCCCACACC AATGCCCTCA 
GGGCTTTCTT TCTCATCCCC TATTCGGGAG TCTGTGGAGC AGCCTCTCAG TGACAGTTAC 
ACATCCCCAG AGGATTCTGA TATTTTCGAT TCTTCGCCCA AGAAGAGAGG ACGTCCAAAG 
ACCAAAATGC CTCGATTAGA TGCACCAGCA CATGGCTGCA CCCCAAACAA GTTGGCACCT 
TCAAAATCCT TCTCCAGTCT GCTCAAGTCT AAGGAAGAGC AGGACCCCCC TGTTTTGCAC 
CCTGAAGTGG ATTTTAATCC ACCTAAAATG ATGGCAAGAA AGCGAGGGCG ACCCAAGCGC 
TCACCTCCAA CTCTTCCTCA AGAAACCCAG CCTCCGACTC TTGCTCCTGA GTCAGGGGAA 
GCATCAGTTG GGGAAAAACC TTTTCGTAAT AAGGGTAATG GGCAGCTCAT TATGAAGACG 
ATCATAGGCA AAATCAATAA AATGAAGACA GTAAAACGGA AGAGGCTCCT CAGCCAAATA 
CTGCTTGGGC CAAAACCAGC AGAGGAGCAG GAATGCTCAA GAAGCAGTGG GCCAACAGGG 
TCTCCGGTTG CTACAAAACC ACAGTCCCTG ACATCACTTG CTGCTACATT TGGTGGCAAA 
TTAGGGCCCC AGATTAATGT AAGCAAAAAG GGGACAATAT ATATGGGTAA AAGAAGGGGT 
CGTAAGCCAA AAACATCAAC TAATCCTTCC CCAGGTGCCC CCACACCCCC TCCTGAGCCT 
TTCTTATCAC CAAACACTTC ATCCCCTCAT CATCACCAGG TGCAGGCTTT ACAAAACCAA 
CCACACCAGT TGCCTCCATC TGAGGTGTTT CCTTCCCCAT CACTATCACA GTCAAGTGGG 
GGCCAAAGCC CCATTAGTGA TGCTAGCTTT GTAGAGCCTG GCTCAGTGCA TTTCTCATCT 
CACCCACACT GCAGTCATTC TCATCAGGTC CACCACTCAT TCTCATTTCC ACCGCCTACT 
TTTTCAGCCC CCTCTGCTCG ATCTGTAGCA AGTGCTGCAA CATCCACTGC ACACCCATCT 
CAGAAGAAGT CCTCTTGCCG GGGTTACCAT CATCACCACC ACCATTACCG GCAGCACTAT 
CATTATCGAA AATTTTCTCC TCCAAGGCCA CTATTGCCAA CGTCTCCAGC TCCACTGAGT 
GAGCTGAAAG AAGCCACACC TTCTCCAGTG AGTGAATCAC ACAGTGAAGA GACTGTACCC 
AGTGACAGTG GCATTGGAAC AGACAATAAT AGCACCTCTG ACCGTGGGGA AAAAGCTGGA 
AGTGCCAATG GCTTACCTGG TGTGGGGGTC TCACAGGGTA TGGCAGGTGG ACTCTTAATG 
CCTGGAGTCA TAGGTCCAGG TATTGGAACG GGAATTGGAC TTTCTTCGAG AGGTCGGAGG 
AGGCACTCAT CTTCAGTTCT TCTAGAACGT CCCTCACCTA CACCCTCACC TCTTGGAGTG 
AGAGCATCAC CTGACCCAAG AAGATCGCAC CCTGCTGCCC CATCTTCTGC TTTGGTTGGA 
CATAAAGAGA AACACAAGCA TAAGTGCAAG CGTCGTGGGC ATGGCTGCCC TAGCTATGAC 
AAACTAAAAA GGCAAAAGCG TAAACGCAAG AAGAAGTACC TGCAGCTACG GTCCCGCAGA 
CAAGACCCAG ATTTTTTGGC TGATTTAGAT GAGATATGTG TGCAGCTTAG TGATATACGA 
ATTTCTCATC GAACACCTAC ACTTCGTTTG GGCAGCAGCA TAGGTTTGGG TGCCGGGGCT 
GGTCCTGGCC GTGGGTCTGG CTTGGGAAGT ACCAGACCTA GCAGCAGCCT TGGAGGCGGA 
ACCAATCCAC CACCCCATCA CTATCTGCAT AGAGATCTAT TACCCACAAT ATTCAGGATA 
AATTTTAGTG GGTATTATTC ACCCCACCCA GCCTACCCAT GTGATTCACT TCATTACGTT 
CGTAAGCCAG ATCTTAAAAA GAAAAGAGGT CGACCACCTA AGCTTCGGGA ATCCATATCA 
GAAGTACCTT TTGTACCTGG TTTAGGCTTT CCCCTCTCAA GTGGAGGTTT CTATCACCCT 
TCTTATGGTG TCCCATACTC TTCTACCCCA CTGGGACTTG GTTACTATCG TGGTTATACC 
CCACCAAGTG CCCTGTACCC CCATCCTCAT CACCAGGCTC CCCACTCTGG CCCATCCCAC 
CATGTTCATT CACCATCTTT TCCTCCACCT CCACCCCCTT CTTATGTGCA TCATCATCAC 
CCATCCCACC TGCTTCTTAA CCCCTCCAAA TTTCACAAGA AGAAACATAA GCTACTTAGG 
CAAGAGTATT TAGGTGGTGG TCGTTCATCC ATGCTCTATC CTGCCATGTC ATCTGAGCTG 
TCATTTGGCT GGCACCACAA ACACAAGCAT AGGCACAAAC ACCGTGATCG CTGTGGAGAA 
GAGGAGGGTG ATGATGGTGG TGGAGTTTTA GGTGGTATAG GAGGCAGAGG TGTTGGAGGT 
GAGGGCTTGG GGGCCGGAAG AGGGGAGAGA GGGAGCGTAG AGTCCCTGCA GCACTGCCGG 
TTTGGACGAG ATGGCTCTGC TGACTCCCGA ACTAACAAAC AGTCAGCTTC TGCCAACTCC 
CCTTCCTCTT CCTCCACATC CTCTGCAGAA CGGTACAAGC GAAAAGAGGC CTCTATGTCT 
TGTCTTGGGC CCTCACGACT GTCTTTGGGC ACTGCAAGTC GGGGACATCA TCCTGTTGAC 
TCCTGGTTCA GAATGGGAAG TTCTGATACT GATTACTCCA AGCTCTCTAG AAACCAGTCT 
TTTTCGGGGT CTGGTGCATT CTCTGAAGGA CATGTGCAAA ATCCAGTGGA TTGCTCAGAG 
AGTGATGAGG AAGATGCTGC TTCCCCCATT GAAGAGCCAG AGATCAGCCA TCACACAAAC 
CTGTTTGCAT CTGCACTATC AAGGACCTCC CTCAAAGGAA GTCGCAGCAA AAAAGGTGGA 
CCATTAGAGA GTCCCAGTCT CTCACGGTTG GACAGGACGG TTAGAAGGGA TCGTTCAACA 
TCTTCAGAGA AAAGAGAAAT TGGGAATGTA TCTAGCTTGC AGAACAGAGT TGGCCAATCC 
AGTGTTCCTG ATGTTTCTGA GGGTCTCCAT CATCGGCACC ACCATCACCA CCACCCTCCT 
CTATCCCTCT CGCACGCTGC CTCTTGCCTG CCGCACTGCA CTCTAGACCA GCCTGGAAAC 
ACTGCACCAC TCCGGCCCTC CAGCCGCCAA GCTAGTCCAT CCACATGCTC CTCCCAGCCT 
GCATGCTGCC TTGATTCCCC ACCTTCACAT CTCTCCCACC GGCCCCCTCC CAAAAACAAC 
CTGCTTCATG TCAACAAGAT CCTCCGTGCA AAGAAGCTCC AAAGGCAAGC ACGTACAGGG 
AACAACATGG TGAAAAAACG GGGTCCAGGA CGTCCCCGCA AGAACCCGCT GCCCTCACCT 
CCTGCAACAC CCCCACCACA GACTTCCGAA CAAGACAACC ACCCATTACC TGAAAGGCAC 
CGAGCAACTG CACTCTGGGA GGCAGATACT GTAGTTGATG CCATTGAGGC TGTCGTACAG 
AACCAGCGCA AAAAGGGACG CAAGCGGAAG CACTGGGAAA GGGAGACTGA AGGAGGTGAT 
GAAGAAGAGG AGGATGCGGA GGAAGAAAGA CAGGTGGAGG ACGAAGAAGA AGAAGAAGAC 
GAAGAACAAG AACAACAGTC AGCTGCTAGA GAAGACGATG TAATGTCGAG AGTTAGAACA 
GAAGGAAGCA GGCAATGGTT GACCCAGGAA GAAGTGCACT GTTTTCGCAG TGCACTGGAG 
GGAAAACCTG ATGGCTCCCC TGAGCGCCCA GGCCCTGCTA CTTCGGAACC AACCCCAAAC 
CCATCAGTGA CCAGCCAACG TGAGAAGAGA GTTGCTAGAC CACCCAAAAA GAAGTTTCAA 
AAGGCGGGAC TTTACTCTGA TGTGTACAAG ACTGATGATC CTCGCAGTCA GCTGCTACAG 
TTAAAGAAAG AGAAGCTAGA ATACACTCCA GGTGAACATG ACTATGGCCT TCTTCCTGCT 
CCCATTCATG TTGGGAAATA CTTGAGACAG AAACGCATTG ATTTCCAGCT GCCTTACGAC 
ATCCTGTGGC TTTGGAAACA TGATCAGCTC TACAAGAGAC CTGACGTGCC TCTCTACAAG 
AAGATCAGAT CCAATGTCTA TGTGGATGTG AAGCCTCTTT CTGGCTATGA AGCCACTACC 
TGTAACTGCA GGTTGCCTGA CGACTCTAGT GAAAAGGGCT GTCAAGATGA TTGTCTAAAC 
AGGATGATTT ATGCCGAGTG CTCTCCAAGT ACGTGTCCGT GTTCCGATCA GTGCGATAAC 
CAGCGGATCC AGAAGCATGA GTGGGTTCAG TGCCTCGAGC GCTTCCGAGC TGAGGGAAAG 
GGCTGGGGCA TCCGGACCAA GCAGCCCCTT CGTGCCGGCC AATTTATCAT CGAGTACTTG 
GGAGAGGTGG TCAGCGAACA AGAGTTCAGA AAATACATTG CGTCTGACTG TAGGTTGCAC 
CAAGTCAAAA TTCCAATGTT GGGGGAAAAT AAAAAGCAGA TTGGTGTGAG TCGCATGATG 
GAGCAGTACT TCTCTCACAG CGGCCACTAC TGCCTGAATC TGGACAGCGG CATGGTCATC 
GATAGCTACC GCATGGGCAA CGAGGCTCGC TTTGTCAACC ACAGCTGCGA GCCCAACTGT 
GAGATGCAGA AATGGTCGGT GAACGGAGTG TACCGAATTG GCCTGTTTGC TCTAAAAGAC 
ATCAACAGCG GAACGGAACT GACATATGAC TACAACTTCC ACTCCTTCAA CACAGAGGAA 
CAACAAGTCT GCAAGTGTGG CTCAGAGGGT TGCCGAGGCA TCATTGGCGG CAAGAGCAAG 
CGAATCAATG GGCTGCCTGC TAAAGGGGCC GGATCTGGAG GCGGAGCCCG GAGACTTGGA 
AGATTGAAAG AGAAACGCAA GTCCAAACAC TCACTGAAGA AGCGGGAGGA AGAGTCCAGT 
GACAGCAGCA AGTTTTACCA GCACCTTTTA ATGAAGCCCA TGTCAAACAG AGAGAGGAAC 
TTTGTGCTGA AACATCGGGT CTTTCTGCTG CGGAATTGGG AGAAGATGCG GGAGAAACAG 
GAGCTTCTGA AGCGTGAGGG AGAGCGGGAG AGGGAGAACA GCGGTCTGTC TCTGTACACC 
CGCTGGGGAG GAGTCATCCG GGATGATGGA AACATCAAAT CAGATGTATT TCTGACTCAG 
TTCTCAGCGC TGCAGACGTC CCGCTCTGTA CGCACACGCA GACTTGCGGC AGCCGAGGAG 
AACACTGAAG TGACACGCAC CGCACGTCTG GCACACATTT TCAAAGAGAT CTGCGATATG 
ATCACTAGCT ACAAGGATTC ATCTGGTCAG CCTCTTGCTG CTCCGCTGCT TAACCTGCCT 
TCTAGGAAGA GGAACACACA ATACTATGAG AAGGTCACCG ATCCTCTAGA TCTCAGCACC 
ATAGATAAGC AGATCCTCAG CGGACACTAC AAAACCGATG AGGCGTTTGA TGCGGACATG 
CTCAAAGTGT TTCGCAATGC TGAGAAGTAC TATGGGCGTA AGTCTGCTGT GGGCCGTGAC 
GTGTGCCGGC TGAGGAAGGC TTATTATGGT GCCCGTCATG AGGCTGCTGT TCAGATCGAT 
GAGATTGTGG GTGAGACGGC AAGTGAAGCG GACAGCTCTG ATTCGCTGGA GAGAGACCAC 
GCCCACCACC ATCACCATGA CGGAGGGGGC AGCCATGATA AGGATGATGA TGTGATCCGC 
TGCATCTGTG GCATGTACAA GGATGAGGGA CTCATGATTC AGTGCGAGAA GTGCATGGTG 
TGGCAGCACT GTGATTGTAT GCGTCTGGAG GCGGATGTGG AGCATTATCT CTGTGAACAG 
TGTGACCCTC GTCCAGTGGA CAGAGAGGTT CCAATGGTGC CTCAACCCAG CTATGCCCAA 
TCAGGATTCA TCTATTACAT ATGCCTGCTT CGCGATGACT TACTGTTGCA TCAGGGCGAT 
TGTGTGTACC TCATGCGGGA CAGCAGACGC ACAACAGAAG GTCAGCCGGT GCGTCAGTCA 
TACCGCCTCC TGTCTCACAT CAACAGAGAC AAACTGGACA TCTTCCGTAT CGAGAAACTC 
TGGAAGAATG AAAAAGGTGA GAGGTTTGCA TTTGGTCATC ATTACTTCCG TCCTCATGAG 
ACGCATCACT CCCCTTCCCG CCGCTTCTAC CACAACGAGC TGTTTCGAGT GCCTCTGTAT 
GAGATCATCC CGCTGGAGGC CGTGGTGGGC ACCTGCTGCG TGCTGGACCT CTACACATAC 
TGCAAGGGTC GTCCAAAAGG GGTAAAGGAG CAGGACGTTT ACATCTGCGA CTACCGGCTG 
GATAAATCCG CACACCTGTT CTACAAGATC CACCGCAACC GTTACCCAGT GTGCACCAAG 
CCCTATGCGT TCAACCACTT CCCCAAGAGA CTGACGCCCA AGAGGGACTT CTCTCCTCAC 
TATGTTCCAG ATAACTACAA GAGGAACGGC GGACGCTCTG CTTGGAAGAG CGAGCGGCCC 
AAAGATGAAG AACCATCATC CTCCAGCACG TCCTGCGAGC GTCTGTCCTC CAGCTTCCCT 
AGAGAAGGAG ACGAGGACGG AGGACGAGGA GCAGAAGGAG ACGCGGAGGC CGCTCAAGAG 
GGATCCAGCA CTCCGAGACC TCAGAGATCC TCGCCACTGG AGAGCAGGAC GGCCGGAGAG 
GAGGATGAGG CGGAGGAGGA AGAGGACAGA GAGAGGAGGA GAGAGATGCA GGAGGAGGAG 
GAGGAGGAGG GATGCAGAGA GAAAGCAGGA GCGCAGGAGC CCATAGACGC ACAGTGCTCC 
TCCTCCATCT CCTCTTCTCC TCTTCATCCC GCCATTCTGG GCAGGAGAGA AGCGCAGAGA 
GAGCGGCTCA ACAAGATCCT GCTCGACCTG CTGCACCGCG CGCCCACTAA GAACGCGATA 
GATGTGACGT ATCTTCTTGA GGAAGGCTCG GGTCGTCGCC TTCGCAGACG GACGCTTGGA 
CTGAGCGAAT TCATTTGCAG AAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159541.1
CDS	759..9725
Translation	MDQRTQGGPSTPPPLPANAPPAEREKEGVGGKKDDEEEKKKREKLRDGAQNEASGAESGTGDQPQQPTQFSVKETSYSEGNVKLKIGLQAKRMKKPPKILENYVCRPAFRATVRHSGGRGGGRANRGAGASDANSNSTSTSNGKEGEKKPSVNRGASQSAAAPAASTPSPSLPPASINTVTPVNGNAPAKRGPPKLACKSEGKADVKSVASSEKPLNLHRPTTESKSHSSGKKNHVPQTNSAPAPSPPAVPPAATSQQDPKIDGILKTPQCNFLEGQKEQERGISSWGTPTVTEKLAQLIATCPPSKCPKPKARKVSPAPAHSSSPPLISNLQRPERAMANRNTYSRAVHLAQPPPVSRPPGRPYGSKNKDSILEKFSGTSKKEESDGLSKGVSGSSSTTTTTTSSALSQNSSRLTTTTSTNESTDINNTDGMKSQSRLGHCPSHSSSAAASPPFSSSSSVERIGSTSCNIGLPGSLTSRPKASPSRIEDFSEKDKERDREKDTDSPRDLSKGITPAITGKQDAKERCSLSAPRNDRGKSSSPVKRSPNQESRVSGVPSPAESTKCTISPSYPSGASRSPPPLCDDTGAISPASPSEQDSKPLKKRRGRRPRWTKAVSRTHKSLHESPSNHQQIPTPMPSGLSFSSPIRESVEQPLSDSYTSPEDSDIFDSSPKKRGRPKTKMPRLDAPAHGCTPNKLAPSKSFSSLLKSKEEQDPPVLHPEVDFNPPKMMARKRGRPKRSPPTLPQETQPPTLAPESGEASVGEKPFRNKGNGQLIMKTIIGKINKMKTVKRKRLLSQILLGPKPAEEQECSRSSGPTGSPVATKPQSLTSLAATFGGKLGPQINVSKKGTIYMGKRRGRKPKTSTNPSPGAPTPPPEPFLSPNTSSPHHHQVQALQNQPHQLPPSEVFPSPSLSQSSGGQSPISDASFVEPGSVHFSSHPHCSHSHQVHHSFSFPPPTFSAPSARSVASAATSTAHPSQKKSSCRGYHHHHHHYRQHYHYRKFSPPRPLLPTSPAPLSELKEATPSPVSESHSEETVPSDSGIGTDNNSTSDRGEKAGSANGLPGVGVSQGMAGGLLMPGVIGPGIGTGIGLSSRGRRRHSSSVLLERPSPTPSPLGVRASPDPRRSHPAAPSSALVGHKEKHKHKCKRRGHGCPSYDKLKRQKRKRKKKYLQLRSRRQDPDFLADLDEICVQLSDIRISHRTPTLRLGSSIGLGAGAGPGRGSGLGSTRPSSSLGGGTNPPPHHYLHRDLLPTIFRINFSGYYSPHPAYPCDSLHYVRKPDLKKKRGRPPKLRESISEVPFVPGLGFPLSSGGFYHPSYGVPYSSTPLGLGYYRGYTPPSALYPHPHHQAPHSGPSHHVHSPSFPPPPPPSYVHHHHPSHLLLNPSKFHKKKHKLLRQEYLGGGRSSMLYPAMSSELSFGWHHKHKHRHKHRDRCGEEEGDDGGGVLGGIGGRGVGGEGLGAGRGERGSVESLQHCRFGRDGSADSRTNKQSASANSPSSSSTSSAERYKRKEASMSCLGPSRLSLGTASRGHHPVDSWFRMGSSDTDYSKLSRNQSFSGSGAFSEGHVQNPVDCSESDEEDAASPIEEPEISHHTNLFASALSRTSLKGSRSKKGGPLESPSLSRLDRTVRRDRSTSSEKREIGNVSSLQNRVGQSSVPDVSEGLHHRHHHHHHPPLSLSHAASCLPHCTLDQPGNTAPLRPSSRQASPSTCSSQPACCLDSPPSHLSHRPPPKNNLLHVNKILRAKKLQRQARTGNNMVKKRGPGRPRKNPLPSPPATPPPQTSEQDNHPLPERHRATALWEADTVVDAIEAVVQNQRKKGRKRKHWERETEGGDEEEEDAEEERQVEDEEEEEDEEQEQQSAAREDDVMSRVRTEGSRQWLTQEEVHCFRSALEGKPDGSPERPGPATSEPTPNPSVTSQREKRVARPPKKKFQKAGLYSDVYKTDDPRSQLLQLKKEKLEYTPGEHDYGLLPAPIHVGKYLRQKRIDFQLPYDILWLWKHDQLYKRPDVPLYKKIRSNVYVDVKPLSGYEATTCNCRLPDDSSEKGCQDDCLNRMIYAECSPSTCPCSDQCDNQRIQKHEWVQCLERFRAEGKGWGIRTKQPLRAGQFIIEYLGEVVSEQEFRKYIASDCRLHQVKIPMLGENKKQIGVSRMMEQYFSHSGHYCLNLDSGMVIDSYRMGNEARFVNHSCEPNCEMQKWSVNGVYRIGLFALKDINSGTELTYDYNFHSFNTEEQQVCKCGSEGCRGIIGGKSKRINGLPAKGAGSGGGARRLGRLKEKRKSKHSLKKREEESSDSSKFYQHLLMKPMSNRERNFVLKHRVFLLRNWEKMREKQELLKREGERERENSGLSLYTRWGGVIRDDGNIKSDVFLTQFSALQTSRSVRTRRLAAAEENTEVTRTARLAHIFKEICDMITSYKDSSGQPLAAPLLNLPSRKRNTQYYEKVTDPLDLSTIDKQILSGHYKTDEAFDADMLKVFRNAEKYYGRKSAVGRDVCRLRKAYYGARHEAAVQIDEIVGETASEADSSDSLERDHAHHHHHDGGGSHDKDDDVIRCICGMYKDEGLMIQCEKCMVWQHCDCMRLEADVEHYLCEQCDPRPVDREVPMVPQPSYAQSGFIYYICLLRDDLLLHQGDCVYLMRDSRRTTEGQPVRQSYRLLSHINRDKLDIFRIEKLWKNEKGERFAFGHHYFRPHETHHSPSRRFYHNELFRVPLYEIIPLEAVVGTCCVLDLYTYCKGRPKGVKEQDVYICDYRLDKSAHLFYKIHRNRYPVCTKPYAFNHFPKRLTPKRDFSPHYVPDNYKRNGGRSAWKSERPKDEEPSSSSTSCERLSSSFPREGDEDGGRGAEGDAEAAQEGSSTPRPQRSSPLESRTAGEEDEAEEEEDRERRREMQEEEEEEGCREKAGAQEPIDAQCSSSISSSPLHPAILGRREAQRERLNKILLDLLHRAPTKNAIDVTYLLEEGSGRRLRRRTLGLSEFICRK

Target ORF information:

RefSeq Version	XM_005159484.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ash1 (absent, small, or homeotic)-like (Drosophila) (ash1l), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159484.3

ORF Insert Sequence:

ATGGATCAGA GGACACAGGG GGGGCCTTCT ACGCCGCCCC CACTCCCTGC CAATGCCCCC 
CCAGCAGAAC GTGAAAAAGA AGGTGTAGGA GGAAAGAAGG ACGATGAAGA AGAGAAAAAG 
AAACGGGAGA AACTAAGAGA TGGGGCACAG AATGAAGCTA GTGGTGCTGA AAGTGGGACT 
GGTGACCAGC CGCAACAACC CACGCAGTTT TCGGTCAAGG AGACCAGCTA CTCTGAGGGT 
AACGTTAAGC TCAAAATAGG CCTGCAGGCC AAACGCATGA AGAAACCTCC TAAGATCCTG 
GAGAACTATG TCTGCCGACC AGCCTTCCGT GCCACTGTAA GGCATAGTGG AGGTAGAGGC 
GGAGGACGTG CTAATCGTGG GGCTGGAGCC AGTGATGCGA ACAGCAACTC TACTAGCACA 
AGTAATGGAA AGGAAGGGGA GAAAAAGCCC TCTGTCAACC GAGGAGCATC TCAGTCAGCT 
GCTGCTCCTG CTGCTTCTAC TCCCTCACCC TCTCTTCCTC CTGCTTCCAT CAACACTGTC 
ACTCCAGTGA ATGGAAATGC TCCAGCAAAA AGGGGTCCTC CAAAACTTGC CTGCAAATCA 
GAGGGAAAGG CTGACGTAAA ATCTGTTGCT TCATCAGAGA AACCTCTTAA TCTCCATCGC 
CCCACGACAG AAAGTAAATC ACACTCCTCT GGAAAGAAAA ACCATGTTCC ACAAACAAAC 
TCTGCCCCTG CCCCATCTCC ACCAGCAGTG CCCCCAGCTG CCACTTCGCA ACAGGACCCT 
AAAATAGATG GAATCTTAAA AACGCCTCAA TGCAATTTTT TGGAGGGGCA GAAGGAACAA 
GAAAGAGGGA TTTCTTCTTG GGGTACCCCT ACAGTAACTG AAAAACTTGC TCAGCTCATA 
GCTACATGCC CACCTTCAAA GTGCCCTAAG CCAAAAGCCC GAAAGGTATC GCCAGCTCCA 
GCACACAGTA GCTCACCTCC GTTAATTTCT AATCTGCAGC GCCCTGAACG GGCAATGGCT 
AATAGAAACA CCTATTCCAG GGCGGTTCAC CTCGCCCAAC CACCACCTGT ATCACGGCCC 
CCTGGTCGCC CATATGGGTC TAAAAACAAA GACAGTATCC TTGAGAAATT TTCTGGAACT 
TCTAAAAAAG AGGAATCAGA TGGACTTTCA AAAGGGGTCT CCGGTAGCAG CAGCACAACC 
ACCACCACCA CTAGTTCAGC ACTGTCTCAA AACAGCAGTC GCTTGACGAC AACAACATCC 
ACAAATGAAA GCACTGACAT TAACAACACA GATGGAATGA AATCTCAGTC TCGTCTTGGG 
CATTGCCCAT CTCATTCTTC ATCCGCAGCT GCTTCACCTC CATTTTCTTC TTCATCCTCC 
GTAGAGAGAA TAGGAAGTAC AAGCTGTAAC ATAGGTTTGC CAGGCTCTCT AACCTCACGC 
CCTAAAGCAA GCCCCTCTCG AATAGAGGAT TTTAGTGAGA AAGACAAAGA ACGTGACCGG 
GAAAAGGACA CAGACAGTCC AAGAGATTTA AGTAAAGGTA TCACTCCAGC TATAACTGGG 
AAGCAGGATG CAAAAGAAAG GTGTTCTTTA TCAGCTCCTC GAAATGACAG GGGTAAGAGC 
TCAAGCCCAG TCAAACGTAG TCCCAATCAG GAAAGTCGCG TTAGTGGGGT ACCAAGTCCT 
GCTGAGTCCA CCAAATGCAC TATTTCTCCT TCATACCCTT CTGGTGCCAG CAGGAGCCCA 
CCACCACTAT GCGATGACAC CGGGGCTATT TCTCCTGCAT CACCATCTGA GCAGGATTCA 
AAACCTCTTA AGAAGCGACG AGGACGACGA CCTCGCTGGA CGAAGGCAGT GAGTCGCACC 
CACAAGTCTT TACATGAGTC TCCTTCTAAC CATCAGCAAA TCCCCACACC AATGCCCTCA 
GGGCTTTCTT TCTCATCCCC TATTCGGGAG TCTGTGGAGC AGCCTCTCAG TGACAGTTAC 
ACATCCCCAG AGGATTCTGA TATTTTCGAT TCTTCGCCCA AGAAGAGAGG ACGTCCAAAG 
ACCAAAATGC CTCGATTAGA TGCACCAGCA CATGGCTGCA CCCCAAACAA GTTGGCACCT 
TCAAAATCCT TCTCCAGTCT GCTCAAGTCT AAGGAAGAGC AGGACCCCCC TGTTTTGCAC 
CCTGAAGTGG ATTTTAATCC ACCTAAAATG ATGGCAAGAA AGCGAGGGCG ACCCAAGCGC 
TCACCTCCAA CTCTTCCTCA AGAAACCCAG CCTCCGACTC TTGCTCCTGA GTCAGGGGAA 
GCATCAGTTG GGGAAAAACC TTTTCGTAAT AAGGGTAATG GGCAGCTCAT TATGAAGACG 
ATCATAGGCA AAATCAATAA AATGAAGACA GTAAAACGGA AGAGGCTCCT CAGCCAAATA 
CTGCTTGGGC CAAAACCAGC AGAGGAGCAG GAATGCTCAA GAAGCAGTGG GCCAACAGGG 
TCTCCGGTTG CTACAAAACC ACAGTCCCTG ACATCACTTG CTGCTACATT TGGTGGCAAA 
TTAGGGCCCC AGATTAATGT AAGCAAAAAG GGGACAATAT ATATGGGTAA AAGAAGGGGT 
CGTAAGCCAA AAACATCAAC TAATCCTTCC CCAGGTGCCC CCACACCCCC TCCTGAGCCT 
TTCTTATCAC CAAACACTTC ATCCCCTCAT CATCACCAGG TGCAGGCTTT ACAAAACCAA 
CCACACCAGT TGCCTCCATC TGAGGTGTTT CCTTCCCCAT CACTATCACA GTCAAGTGGG 
GGCCAAAGCC CCATTAGTGA TGCTAGCTTT GTAGAGCCTG GCTCAGTGCA TTTCTCATCT 
CACCCACACT GCAGTCATTC TCATCAGGTC CACCACTCAT TCTCATTTCC ACCGCCTACT 
TTTTCAGCCC CCTCTGCTCG ATCTGTAGCA AGTGCTGCAA CATCCACTGC ACACCCATCT 
CAGAAGAAGT CCTCTTGCCG GGGTTACCAT CATCACCACC ACCATTACCG GCAGCACTAT 
CATTATCGAA AATTTTCTCC TCCAAGGCCA CTATTGCCAA CGTCTCCAGC TCCACTGAGT 
GAGCTGAAAG AAGCCACACC TTCTCCAGTG AGTGAATCAC ACAGTGAAGA GACTGTACCC 
AGTGACAGTG GCATTGGAAC AGACAATAAT AGCACCTCTG ACCGTGGGGA AAAAGCTGGA 
AGTGCCAATG GCTTACCTGG TGTGGGGGTC TCACAGGGTA TGGCAGGTGG ACTCTTAATG 
CCTGGAGTCA TAGGTCCAGG TATTGGAACG GGAATTGGAC TTTCTTCGAG AGGTCGGAGG 
AGGCACTCAT CTTCAGTTCT TCTAGAACGT CCCTCACCTA CACCCTCACC TCTTGGAGTG 
AGAGCATCAC CTGACCCAAG AAGATCGCAC CCTGCTGCCC CATCTTCTGC TTTGGTTGGA 
CATAAAGAGA AACACAAGCA TAAGTGCAAG CGTCGTGGGC ATGGCTGCCC TAGCTATGAC 
AAACTAAAAA GGCAAAAGCG TAAACGCAAG AAGAAGTACC TGCAGCTACG GTCCCGCAGA 
CAAGACCCAG ATTTTTTGGC TGATTTAGAT GAGATATGTG TGCAGCTTAG TGATATACGA 
ATTTCTCATC GAACACCTAC ACTTCGTTTG GGCAGCAGCA TAGGTTTGGG TGCCGGGGCT 
GGTCCTGGCC GTGGGTCTGG CTTGGGAAGT ACCAGACCTA GCAGCAGCCT TGGAGGCGGA 
ACCAATCCAC CACCCCATCA CTATCTGCAT AGAGATCTAT TACCCACAAT ATTCAGGATA 
AATTTTAGTG GGTATTATTC ACCCCACCCA GCCTACCCAT GTGATTCACT TCATTACGTT 
CGTAAGCCAG ATCTTAAAAA GAAAAGAGGT CGACCACCTA AGCTTCGGGA ATCCATATCA 
GAAGTACCTT TTGTACCTGG TTTAGGCTTT CCCCTCTCAA GTGGAGGTTT CTATCACCCT 
TCTTATGGTG TCCCATACTC TTCTACCCCA CTGGGACTTG GTTACTATCG TGGTTATACC 
CCACCAAGTG CCCTGTACCC CCATCCTCAT CACCAGGCTC CCCACTCTGG CCCATCCCAC 
CATGTTCATT CACCATCTTT TCCTCCACCT CCACCCCCTT CTTATGTGCA TCATCATCAC 
CCATCCCACC TGCTTCTTAA CCCCTCCAAA TTTCACAAGA AGAAACATAA GCTACTTAGG 
CAAGAGTATT TAGGTGGTGG TCGTTCATCC ATGCTCTATC CTGCCATGTC ATCTGAGCTG 
TCATTTGGCT GGCACCACAA ACACAAGCAT AGGCACAAAC ACCGTGATCG CTGTGGAGAA 
GAGGAGGGTG ATGATGGTGG TGGAGTTTTA GGTGGTATAG GAGGCAGAGG TGTTGGAGGT 
GAGGGCTTGG GGGCCGGAAG AGGGGAGAGA GGGAGCGTAG AGTCCCTGCA GCACTGCCGG 
TTTGGACGAG ATGGCTCTGC TGACTCCCGA ACTAACAAAC AGTCAGCTTC TGCCAACTCC 
CCTTCCTCTT CCTCCACATC CTCTGCAGAA CGGTACAAGC GAAAAGAGGC CTCTATGTCT 
TGTCTTGGGC CCTCACGACT GTCTTTGGGC ACTGCAAGTC GGGGACATCA TCCTGTTGAC 
TCCTGGTTCA GAATGGGAAG TTCTGATACT GATTACTCCA AGCTCTCTAG AAACCAGTCT 
TTTTCGGGGT CTGGTGCATT CTCTGAAGGA CATGTGCAAA ATCCAGTGGA TTGCTCAGAG 
AGTGATGAGG AAGATGCTGC TTCCCCCATT GAAGAGCCAG AGATCAGCCA TCACACAAAC 
CTGTTTGCAT CTGCACTATC AAGGACCTCC CTCAAAGGAA GTCGCAGCAA AAAAGGTGGA 
CCATTAGAGA GTCCCAGTCT CTCACGGTTG GACAGGACGG TTAGAAGGGA TCGTTCAACA 
TCTTCAGAGA AAAGAGAAAT TGGGAATGTA TCTAGCTTGC AGAACAGAGT TGGCCAATCC 
AGTGTTCCTG ATGTTTCTGA GGGTCTCCAT CATCGGCACC ACCATCACCA CCACCCTCCT 
CTATCCCTCT CGCACGCTGC CTCTTGCCTG CCGCACTGCA CTCTAGACCA GCCTGGAAAC 
ACTGCACCAC TCCGGCCCTC CAGCCGCCAA GCTAGTCCAT CCACATGCTC CTCCCAGCCT 
GCATGCTGCC TTGATTCCCC ACCTTCACAT CTCTCCCACC GGCCCCCTCC CAAAAACAAC 
CTGCTTCATG TCAACAAGAT CCTCCGTGCA AAGAAGCTCC AAAGGCAAGC ACGTACAGGG 
AACAACATGG TGAAAAAACG GGGTCCAGGA CGTCCCCGCA AGAACCCGCT GCCCTCACCT 
CCTGCAACAC CCCCACCACA GACTTCCGAA CAAGACAACC ACCCATTACC TGAAAGGCAC 
CGAGCAACTG CACTCTGGGA GGCAGATACT GTAGTTGATG CCATTGAGGC TGTCGTACAG 
AACCAGCGCA AAAAGGGACG CAAGCGGAAG CACTGGGAAA GGGAGACTGA AGGAGGTGAT 
GAAGAAGAGG AGGATGCGGA GGAAGAAAGA CAGGTGGAGG ACGAAGAAGA AGAAGAAGAC 
GAAGAACAAG AACAACAGTC AGCTGCTAGA GAAGACGATG TAATGTCGAG AGTTAGAACA 
GAAGGAAGCA GGCAATGGTT GACCCAGGAA GAAGTGCACT GTTTTCGCAG TGCACTGGAG 
GGAAAACCTG ATGGCTCCCC TGAGCGCCCA GGCCCTGCTA CTTCGGAACC AACCCCAAAC 
CCATCAGTGA CCAGCCAACG TGAGAAGAGA GTTGCTAGAC CACCCAAAAA GAAGTTTCAA 
AAGGCGGGAC TTTACTCTGA TGTGTACAAG ACTGATGATC CTCGCAGTCA GCTGCTACAG 
TTAAAGAAAG AGAAGCTAGA ATACACTCCA GGTGAACATG ACTATGGCCT TCTTCCTGCT 
CCCATTCATG TTGGGAAATA CTTGAGACAG AAACGCATTG ATTTCCAGCT GCCTTACGAC 
ATCCTGTGGC TTTGGAAACA TGATCAGCTC TACAAGAGAC CTGACGTGCC TCTCTACAAG 
AAGATCAGAT CCAATGTCTA TGTGGATGTG AAGCCTCTTT CTGGCTATGA AGCCACTACC 
TGTAACTGCA GGTTGCCTGA CGACTCTAGT GAAAAGGGCT GTCAAGATGA TTGTCTAAAC 
AGGATGATTT ATGCCGAGTG CTCTCCAAGT ACGTGTCCGT GTTCCGATCA GTGCGATAAC 
CAGCGGATCC AGAAGCATGA GTGGGTTCAG TGCCTCGAGC GCTTCCGAGC TGAGGGAAAG 
GGCTGGGGCA TCCGGACCAA GCAGCCCCTT CGTGCCGGCC AATTTATCAT CGAGTACTTG 
GGAGAGGTGG TCAGCGAACA AGAGTTCAGA AAATACATTG CGTCTGACTG TAGGTTGCAC 
CAAGTCAAAA TTCCAATGTT GGGGGAAAAT AAAAAGCAGA TTGGTGTGAG TCGCATGATG 
GAGCAGTACT TCTCTCACAG CGGCCACTAC TGCCTGAATC TGGACAGCGG CATGGTCATC 
GATAGCTACC GCATGGGCAA CGAGGCTCGC TTTGTCAACC ACAGCTGCGA GCCCAACTGT 
GAGATGCAGA AATGGTCGGT GAACGGAGTG TACCGAATTG GCCTGTTTGC TCTAAAAGAC 
ATCAACAGCG GAACGGAACT GACATATGAC TACAACTTCC ACTCCTTCAA CACAGAGGAA 
CAACAAGTCT GCAAGTGTGG CTCAGAGGGT TGCCGAGGCA TCATTGGCGG CAAGAGCAAG 
CGAATCAATG GGCTGCCTGC TAAAGGGGCC GGATCTGGAG GCGGAGCCCG GAGACTTGGA 
AGATTGAAAG AGAAACGCAA GTCCAAACAC TCACTGAAGA AGCGGGAGGA AGAGTCCAGT 
GACAGCAGCA AGTTTTACCA GCACCTTTTA ATGAAGCCCA TGTCAAACAG AGAGAGGAAC 
TTTGTGCTGA AACATCGGGT CTTTCTGCTG CGGAATTGGG AGAAGATGCG GGAGAAACAG 
GAGCTTCTGA AGCGTGAGGG AGAGCGGGAG AGGGAGAACA GCGGTCTGTC TCTGTACACC 
CGCTGGGGAG GAGTCATCCG GGATGATGGA AACATCAAAT CAGATGTATT TCTGACTCAG 
TTCTCAGCGC TGCAGACGTC CCGCTCTGTA CGCACACGCA GACTTGCGGC AGCCGAGGAG 
AACACTGAAG TGACACGCAC CGCACGTCTG GCACACATTT TCAAAGAGAT CTGCGATATG 
ATCACTAGCT ACAAGGATTC ATCTGGTCAG CCTCTTGCTG CTCCGCTGCT TAACCTGCCT 
TCTAGGAAGA GGAACACACA ATACTATGAG AAGGTCACCG ATCCTCTAGA TCTCAGCACC 
ATAGATAAGC AGATCCTCAG CGGACACTAC AAAACCGATG AGGCGTTTGA TGCGGACATG 
CTCAAAGTGT TTCGCAATGC TGAGAAGTAC TATGGGCGTA AGTCTGCTGT GGGCCGTGAC 
GTGTGCCGGC TGAGGAAGGC TTATTATGGT GCCCGTCATG AGGCTGCTGT TCAGATCGAT 
GAGATTGTGG GTGAGACGGC AAGTGAAGCG GACAGCTCTG ATTCGCTGGA GAGAGACCAC 
GCCCACCACC ATCACCATGA CGGAGGGGGC AGCCATGATA AGGATGATGA TGTGATCCGC 
TGCATCTGTG GCATGTACAA GGATGAGGGA CTCATGATTC AGTGCGAGAA GTGCATGGTG 
TGGCAGCACT GTGATTGTAT GCGTCTGGAG GCGGATGTGG AGCATTATCT CTGTGAACAG 
TGTGACCCTC GTCCAGTGGA CAGAGAGGTT CCAATGGTGC CTCAACCCAG CTATGCCCAA 
TCAGGATTCA TCTATTACAT ATGCCTGCTT CGCGATGACT TACTGTTGCA TCAGGGCGAT 
TGTGTGTACC TCATGCGGGA CAGCAGACGC ACAACAGAAG GTCAGCCGGT GCGTCAGTCA 
TACCGCCTCC TGTCTCACAT CAACAGAGAC AAACTGGACA TCTTCCGTAT CGAGAAACTC 
TGGAAGAATG AAAAAGGTGA GAGGTTTGCA TTTGGTCATC ATTACTTCCG TCCTCATGAG 
ACGCATCACT CCCCTTCCCG CCGCTTCTAC CACAACGAGC TGTTTCGAGT GCCTCTGTAT 
GAGATCATCC CGCTGGAGGC CGTGGTGGGC ACCTGCTGCG TGCTGGACCT CTACACATAC 
TGCAAGGGTC GTCCAAAAGG GGTAAAGGAG CAGGACGTTT ACATCTGCGA CTACCGGCTG 
GATAAATCCG CACACCTGTT CTACAAGATC CACCGCAACC GTTACCCAGT GTGCACCAAG 
CCCTATGCGT TCAACCACTT CCCCAAGAGA CTGACGCCCA AGAGGGACTT CTCTCCTCAC 
TATGTTCCAG ATAACTACAA GAGGAACGGC GGACGCTCTG CTTGGAAGAG CGAGCGGCCC 
AAAGATGAAG AACCATCATC CTCCAGCACG TCCTGCGAGC GTCTGTCCTC CAGCTTCCCT 
AGAGAAGGAG ACGAGGACGG AGGACGAGGA GCAGAAGGAG ACGCGGAGGC CGCTCAAGAG 
GGATCCAGCA CTCCGAGACC TCAGAGATCC TCGCCACTGG AGAGCAGGAC GGCCGGAGAG 
GAGGATGAGG CGGAGGAGGA AGAGGACAGA GAGAGGAGGA GAGAGATGCA GGAGGAGGAG 
GAGGAGGAGG GATGCAGAGA GAAAGCAGGA GCGCAGGAGC CCATAGACGC ACAGTGCTCC 
TCCTCCATCT CCTCTTCTCC TCTTCATCCC GCCATTCTGG GCAGGAGAGA AGCGCAGAGA 
GAGCGGCTCA ACAAGATCCT GCTCGACCTG CTGCACCGCG CGCCCACTAA GAACGCGATA 
GATGTGACGT ATCTTCTTGA GGAAGGCTCG GGTCGTCGCC TTCGCAGACG GACGCTTGGA 
CTGAGCGAAT TCATTTGCAG AAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ash1l ( XM_005159484.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159541.1 Danio rerio ash1 (absent, small, or homeotic)-like (Drosophila) (ash1l), transcript variant X2, mRNA.

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CloneID	ODa23935
Clone ID Related Accession (Same CDS sequence)	XM_005159483.3 , XM_005159484.3 , XM_005159484.4 , XM_005159483.4
Accession Version	XM_005159484.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8967bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	probable histone-lysine N-methyltransferase ASH1L isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159484.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCAGA GGACACAGGG GGGGCCTTCT ACGCCGCCCC CACTCCCTGC CAATGCCCCC 
CCAGCAGAAC GTGAAAAAGA AGGTGTAGGA GGAAAGAAGG ACGATGAAGA AGAGAAAAAG 
AAACGGGAGA AACTAAGAGA TGGGGCACAG AATGAAGCTA GTGGTGCTGA AAGTGGGACT 
GGTGACCAGC CGCAACAACC CACGCAGTTT TCGGTCAAGG AGACCAGCTA CTCTGAGGGT 
AACGTTAAGC TCAAAATAGG CCTGCAGGCC AAACGCATGA AGAAACCTCC TAAGATCCTG 
GAGAACTATG TCTGCCGACC AGCCTTCCGT GCCACTGTAA GGCATAGTGG AGGTAGAGGC 
GGAGGACGTG CTAATCGTGG GGCTGGAGCC AGTGATGCGA ACAGCAACTC TACTAGCACA 
AGTAATGGAA AGGAAGGGGA GAAAAAGCCC TCTGTCAACC GAGGAGCATC TCAGTCAGCT 
GCTGCTCCTG CTGCTTCTAC TCCCTCACCC TCTCTTCCTC CTGCTTCCAT CAACACTGTC 
ACTCCAGTGA ATGGAAATGC TCCAGCAAAA AGGGGTCCTC CAAAACTTGC CTGCAAATCA 
GAGGGAAAGG CTGACGTAAA ATCTGTTGCT TCATCAGAGA AACCTCTTAA TCTCCATCGC 
CCCACGACAG AAAGTAAATC ACACTCCTCT GGAAAGAAAA ACCATGTTCC ACAAACAAAC 
TCTGCCCCTG CCCCATCTCC ACCAGCAGTG CCCCCAGCTG CCACTTCGCA ACAGGACCCT 
AAAATAGATG GAATCTTAAA AACGCCTCAA TGCAATTTTT TGGAGGGGCA GAAGGAACAA 
GAAAGAGGGA TTTCTTCTTG GGGTACCCCT ACAGTAACTG AAAAACTTGC TCAGCTCATA 
GCTACATGCC CACCTTCAAA GTGCCCTAAG CCAAAAGCCC GAAAGGTATC GCCAGCTCCA 
GCACACAGTA GCTCACCTCC GTTAATTTCT AATCTGCAGC GCCCTGAACG GGCAATGGCT 
AATAGAAACA CCTATTCCAG GGCGGTTCAC CTCGCCCAAC CACCACCTGT ATCACGGCCC 
CCTGGTCGCC CATATGGGTC TAAAAACAAA GACAGTATCC TTGAGAAATT TTCTGGAACT 
TCTAAAAAAG AGGAATCAGA TGGACTTTCA AAAGGGGTCT CCGGTAGCAG CAGCACAACC 
ACCACCACCA CTAGTTCAGC ACTGTCTCAA AACAGCAGTC GCTTGACGAC AACAACATCC 
ACAAATGAAA GCACTGACAT TAACAACACA GATGGAATGA AATCTCAGTC TCGTCTTGGG 
CATTGCCCAT CTCATTCTTC ATCCGCAGCT GCTTCACCTC CATTTTCTTC TTCATCCTCC 
GTAGAGAGAA TAGGAAGTAC AAGCTGTAAC ATAGGTTTGC CAGGCTCTCT AACCTCACGC 
CCTAAAGCAA GCCCCTCTCG AATAGAGGAT TTTAGTGAGA AAGACAAAGA ACGTGACCGG 
GAAAAGGACA CAGACAGTCC AAGAGATTTA AGTAAAGGTA TCACTCCAGC TATAACTGGG 
AAGCAGGATG CAAAAGAAAG GTGTTCTTTA TCAGCTCCTC GAAATGACAG GGGTAAGAGC 
TCAAGCCCAG TCAAACGTAG TCCCAATCAG GAAAGTCGCG TTAGTGGGGT ACCAAGTCCT 
GCTGAGTCCA CCAAATGCAC TATTTCTCCT TCATACCCTT CTGGTGCCAG CAGGAGCCCA 
CCACCACTAT GCGATGACAC CGGGGCTATT TCTCCTGCAT CACCATCTGA GCAGGATTCA 
AAACCTCTTA AGAAGCGACG AGGACGACGA CCTCGCTGGA CGAAGGCAGT GAGTCGCACC 
CACAAGTCTT TACATGAGTC TCCTTCTAAC CATCAGCAAA TCCCCACACC AATGCCCTCA 
GGGCTTTCTT TCTCATCCCC TATTCGGGAG TCTGTGGAGC AGCCTCTCAG TGACAGTTAC 
ACATCCCCAG AGGATTCTGA TATTTTCGAT TCTTCGCCCA AGAAGAGAGG ACGTCCAAAG 
ACCAAAATGC CTCGATTAGA TGCACCAGCA CATGGCTGCA CCCCAAACAA GTTGGCACCT 
TCAAAATCCT TCTCCAGTCT GCTCAAGTCT AAGGAAGAGC AGGACCCCCC TGTTTTGCAC 
CCTGAAGTGG ATTTTAATCC ACCTAAAATG ATGGCAAGAA AGCGAGGGCG ACCCAAGCGC 
TCACCTCCAA CTCTTCCTCA AGAAACCCAG CCTCCGACTC TTGCTCCTGA GTCAGGGGAA 
GCATCAGTTG GGGAAAAACC TTTTCGTAAT AAGGGTAATG GGCAGCTCAT TATGAAGACG 
ATCATAGGCA AAATCAATAA AATGAAGACA GTAAAACGGA AGAGGCTCCT CAGCCAAATA 
CTGCTTGGGC CAAAACCAGC AGAGGAGCAG GAATGCTCAA GAAGCAGTGG GCCAACAGGG 
TCTCCGGTTG CTACAAAACC ACAGTCCCTG ACATCACTTG CTGCTACATT TGGTGGCAAA 
TTAGGGCCCC AGATTAATGT AAGCAAAAAG GGGACAATAT ATATGGGTAA AAGAAGGGGT 
CGTAAGCCAA AAACATCAAC TAATCCTTCC CCAGGTGCCC CCACACCCCC TCCTGAGCCT 
TTCTTATCAC CAAACACTTC ATCCCCTCAT CATCACCAGG TGCAGGCTTT ACAAAACCAA 
CCACACCAGT TGCCTCCATC TGAGGTGTTT CCTTCCCCAT CACTATCACA GTCAAGTGGG 
GGCCAAAGCC CCATTAGTGA TGCTAGCTTT GTAGAGCCTG GCTCAGTGCA TTTCTCATCT 
CACCCACACT GCAGTCATTC TCATCAGGTC CACCACTCAT TCTCATTTCC ACCGCCTACT 
TTTTCAGCCC CCTCTGCTCG ATCTGTAGCA AGTGCTGCAA CATCCACTGC ACACCCATCT 
CAGAAGAAGT CCTCTTGCCG GGGTTACCAT CATCACCACC ACCATTACCG GCAGCACTAT 
CATTATCGAA AATTTTCTCC TCCAAGGCCA CTATTGCCAA CGTCTCCAGC TCCACTGAGT 
GAGCTGAAAG AAGCCACACC TTCTCCAGTG AGTGAATCAC ACAGTGAAGA GACTGTACCC 
AGTGACAGTG GCATTGGAAC AGACAATAAT AGCACCTCTG ACCGTGGGGA AAAAGCTGGA 
AGTGCCAATG GCTTACCTGG TGTGGGGGTC TCACAGGGTA TGGCAGGTGG ACTCTTAATG 
CCTGGAGTCA TAGGTCCAGG TATTGGAACG GGAATTGGAC TTTCTTCGAG AGGTCGGAGG 
AGGCACTCAT CTTCAGTTCT TCTAGAACGT CCCTCACCTA CACCCTCACC TCTTGGAGTG 
AGAGCATCAC CTGACCCAAG AAGATCGCAC CCTGCTGCCC CATCTTCTGC TTTGGTTGGA 
CATAAAGAGA AACACAAGCA TAAGTGCAAG CGTCGTGGGC ATGGCTGCCC TAGCTATGAC 
AAACTAAAAA GGCAAAAGCG TAAACGCAAG AAGAAGTACC TGCAGCTACG GTCCCGCAGA 
CAAGACCCAG ATTTTTTGGC TGATTTAGAT GAGATATGTG TGCAGCTTAG TGATATACGA 
ATTTCTCATC GAACACCTAC ACTTCGTTTG GGCAGCAGCA TAGGTTTGGG TGCCGGGGCT 
GGTCCTGGCC GTGGGTCTGG CTTGGGAAGT ACCAGACCTA GCAGCAGCCT TGGAGGCGGA 
ACCAATCCAC CACCCCATCA CTATCTGCAT AGAGATCTAT TACCCACAAT ATTCAGGATA 
AATTTTAGTG GGTATTATTC ACCCCACCCA GCCTACCCAT GTGATTCACT TCATTACGTT 
CGTAAGCCAG ATCTTAAAAA GAAAAGAGGT CGACCACCTA AGCTTCGGGA ATCCATATCA 
GAAGTACCTT TTGTACCTGG TTTAGGCTTT CCCCTCTCAA GTGGAGGTTT CTATCACCCT 
TCTTATGGTG TCCCATACTC TTCTACCCCA CTGGGACTTG GTTACTATCG TGGTTATACC 
CCACCAAGTG CCCTGTACCC CCATCCTCAT CACCAGGCTC CCCACTCTGG CCCATCCCAC 
CATGTTCATT CACCATCTTT TCCTCCACCT CCACCCCCTT CTTATGTGCA TCATCATCAC 
CCATCCCACC TGCTTCTTAA CCCCTCCAAA TTTCACAAGA AGAAACATAA GCTACTTAGG 
CAAGAGTATT TAGGTGGTGG TCGTTCATCC ATGCTCTATC CTGCCATGTC ATCTGAGCTG 
TCATTTGGCT GGCACCACAA ACACAAGCAT AGGCACAAAC ACCGTGATCG CTGTGGAGAA 
GAGGAGGGTG ATGATGGTGG TGGAGTTTTA GGTGGTATAG GAGGCAGAGG TGTTGGAGGT 
GAGGGCTTGG GGGCCGGAAG AGGGGAGAGA GGGAGCGTAG AGTCCCTGCA GCACTGCCGG 
TTTGGACGAG ATGGCTCTGC TGACTCCCGA ACTAACAAAC AGTCAGCTTC TGCCAACTCC 
CCTTCCTCTT CCTCCACATC CTCTGCAGAA CGGTACAAGC GAAAAGAGGC CTCTATGTCT 
TGTCTTGGGC CCTCACGACT GTCTTTGGGC ACTGCAAGTC GGGGACATCA TCCTGTTGAC 
TCCTGGTTCA GAATGGGAAG TTCTGATACT GATTACTCCA AGCTCTCTAG AAACCAGTCT 
TTTTCGGGGT CTGGTGCATT CTCTGAAGGA CATGTGCAAA ATCCAGTGGA TTGCTCAGAG 
AGTGATGAGG AAGATGCTGC TTCCCCCATT GAAGAGCCAG AGATCAGCCA TCACACAAAC 
CTGTTTGCAT CTGCACTATC AAGGACCTCC CTCAAAGGAA GTCGCAGCAA AAAAGGTGGA 
CCATTAGAGA GTCCCAGTCT CTCACGGTTG GACAGGACGG TTAGAAGGGA TCGTTCAACA 
TCTTCAGAGA AAAGAGAAAT TGGGAATGTA TCTAGCTTGC AGAACAGAGT TGGCCAATCC 
AGTGTTCCTG ATGTTTCTGA GGGTCTCCAT CATCGGCACC ACCATCACCA CCACCCTCCT 
CTATCCCTCT CGCACGCTGC CTCTTGCCTG CCGCACTGCA CTCTAGACCA GCCTGGAAAC 
ACTGCACCAC TCCGGCCCTC CAGCCGCCAA GCTAGTCCAT CCACATGCTC CTCCCAGCCT 
GCATGCTGCC TTGATTCCCC ACCTTCACAT CTCTCCCACC GGCCCCCTCC CAAAAACAAC 
CTGCTTCATG TCAACAAGAT CCTCCGTGCA AAGAAGCTCC AAAGGCAAGC ACGTACAGGG 
AACAACATGG TGAAAAAACG GGGTCCAGGA CGTCCCCGCA AGAACCCGCT GCCCTCACCT 
CCTGCAACAC CCCCACCACA GACTTCCGAA CAAGACAACC ACCCATTACC TGAAAGGCAC 
CGAGCAACTG CACTCTGGGA GGCAGATACT GTAGTTGATG CCATTGAGGC TGTCGTACAG 
AACCAGCGCA AAAAGGGACG CAAGCGGAAG CACTGGGAAA GGGAGACTGA AGGAGGTGAT 
GAAGAAGAGG AGGATGCGGA GGAAGAAAGA CAGGTGGAGG ACGAAGAAGA AGAAGAAGAC 
GAAGAACAAG AACAACAGTC AGCTGCTAGA GAAGACGATG TAATGTCGAG AGTTAGAACA 
GAAGGAAGCA GGCAATGGTT GACCCAGGAA GAAGTGCACT GTTTTCGCAG TGCACTGGAG 
GGAAAACCTG ATGGCTCCCC TGAGCGCCCA GGCCCTGCTA CTTCGGAACC AACCCCAAAC 
CCATCAGTGA CCAGCCAACG TGAGAAGAGA GTTGCTAGAC CACCCAAAAA GAAGTTTCAA 
AAGGCGGGAC TTTACTCTGA TGTGTACAAG ACTGATGATC CTCGCAGTCA GCTGCTACAG 
TTAAAGAAAG AGAAGCTAGA ATACACTCCA GGTGAACATG ACTATGGCCT TCTTCCTGCT 
CCCATTCATG TTGGGAAATA CTTGAGACAG AAACGCATTG ATTTCCAGCT GCCTTACGAC 
ATCCTGTGGC TTTGGAAACA TGATCAGCTC TACAAGAGAC CTGACGTGCC TCTCTACAAG 
AAGATCAGAT CCAATGTCTA TGTGGATGTG AAGCCTCTTT CTGGCTATGA AGCCACTACC 
TGTAACTGCA GGTTGCCTGA CGACTCTAGT GAAAAGGGCT GTCAAGATGA TTGTCTAAAC 
AGGATGATTT ATGCCGAGTG CTCTCCAAGT ACGTGTCCGT GTTCCGATCA GTGCGATAAC 
CAGCGGATCC AGAAGCATGA GTGGGTTCAG TGCCTCGAGC GCTTCCGAGC TGAGGGAAAG 
GGCTGGGGCA TCCGGACCAA GCAGCCCCTT CGTGCCGGCC AATTTATCAT CGAGTACTTG 
GGAGAGGTGG TCAGCGAACA AGAGTTCAGA AAATACATTG CGTCTGACTG TAGGTTGCAC 
CAAGTCAAAA TTCCAATGTT GGGGGAAAAT AAAAAGCAGA TTGGTGTGAG TCGCATGATG 
GAGCAGTACT TCTCTCACAG CGGCCACTAC TGCCTGAATC TGGACAGCGG CATGGTCATC 
GATAGCTACC GCATGGGCAA CGAGGCTCGC TTTGTCAACC ACAGCTGCGA GCCCAACTGT 
GAGATGCAGA AATGGTCGGT GAACGGAGTG TACCGAATTG GCCTGTTTGC TCTAAAAGAC 
ATCAACAGCG GAACGGAACT GACATATGAC TACAACTTCC ACTCCTTCAA CACAGAGGAA 
CAACAAGTCT GCAAGTGTGG CTCAGAGGGT TGCCGAGGCA TCATTGGCGG CAAGAGCAAG 
CGAATCAATG GGCTGCCTGC TAAAGGGGCC GGATCTGGAG GCGGAGCCCG GAGACTTGGA 
AGATTGAAAG AGAAACGCAA GTCCAAACAC TCACTGAAGA AGCGGGAGGA AGAGTCCAGT 
GACAGCAGCA AGTTTTACCA GCACCTTTTA ATGAAGCCCA TGTCAAACAG AGAGAGGAAC 
TTTGTGCTGA AACATCGGGT CTTTCTGCTG CGGAATTGGG AGAAGATGCG GGAGAAACAG 
GAGCTTCTGA AGCGTGAGGG AGAGCGGGAG AGGGAGAACA GCGGTCTGTC TCTGTACACC 
CGCTGGGGAG GAGTCATCCG GGATGATGGA AACATCAAAT CAGATGTATT TCTGACTCAG 
TTCTCAGCGC TGCAGACGTC CCGCTCTGTA CGCACACGCA GACTTGCGGC AGCCGAGGAG 
AACACTGAAG TGACACGCAC CGCACGTCTG GCACACATTT TCAAAGAGAT CTGCGATATG 
ATCACTAGCT ACAAGGATTC ATCTGGTCAG CCTCTTGCTG CTCCGCTGCT TAACCTGCCT 
TCTAGGAAGA GGAACACACA ATACTATGAG AAGGTCACCG ATCCTCTAGA TCTCAGCACC 
ATAGATAAGC AGATCCTCAG CGGACACTAC AAAACCGATG AGGCGTTTGA TGCGGACATG 
CTCAAAGTGT TTCGCAATGC TGAGAAGTAC TATGGGCGTA AGTCTGCTGT GGGCCGTGAC 
GTGTGCCGGC TGAGGAAGGC TTATTATGGT GCCCGTCATG AGGCTGCTGT TCAGATCGAT 
GAGATTGTGG GTGAGACGGC AAGTGAAGCG GACAGCTCTG ATTCGCTGGA GAGAGACCAC 
GCCCACCACC ATCACCATGA CGGAGGGGGC AGCCATGATA AGGATGATGA TGTGATCCGC 
TGCATCTGTG GCATGTACAA GGATGAGGGA CTCATGATTC AGTGCGAGAA GTGCATGGTG 
TGGCAGCACT GTGATTGTAT GCGTCTGGAG GCGGATGTGG AGCATTATCT CTGTGAACAG 
TGTGACCCTC GTCCAGTGGA CAGAGAGGTT CCAATGGTGC CTCAACCCAG CTATGCCCAA 
TCAGGATTCA TCTATTACAT ATGCCTGCTT CGCGATGACT TACTGTTGCA TCAGGGCGAT 
TGTGTGTACC TCATGCGGGA CAGCAGACGC ACAACAGAAG GTCAGCCGGT GCGTCAGTCA 
TACCGCCTCC TGTCTCACAT CAACAGAGAC AAACTGGACA TCTTCCGTAT CGAGAAACTC 
TGGAAGAATG AAAAAGGTGA GAGGTTTGCA TTTGGTCATC ATTACTTCCG TCCTCATGAG 
ACGCATCACT CCCCTTCCCG CCGCTTCTAC CACAACGAGC TGTTTCGAGT GCCTCTGTAT 
GAGATCATCC CGCTGGAGGC CGTGGTGGGC ACCTGCTGCG TGCTGGACCT CTACACATAC 
TGCAAGGGTC GTCCAAAAGG GGTAAAGGAG CAGGACGTTT ACATCTGCGA CTACCGGCTG 
GATAAATCCG CACACCTGTT CTACAAGATC CACCGCAACC GTTACCCAGT GTGCACCAAG 
CCCTATGCGT TCAACCACTT CCCCAAGAGA CTGACGCCCA AGAGGGACTT CTCTCCTCAC 
TATGTTCCAG ATAACTACAA GAGGAACGGC GGACGCTCTG CTTGGAAGAG CGAGCGGCCC 
AAAGATGAAG AACCATCATC CTCCAGCACG TCCTGCGAGC GTCTGTCCTC CAGCTTCCCT 
AGAGAAGGAG ACGAGGACGG AGGACGAGGA GCAGAAGGAG ACGCGGAGGC CGCTCAAGAG 
GGATCCAGCA CTCCGAGACC TCAGAGATCC TCGCCACTGG AGAGCAGGAC GGCCGGAGAG 
GAGGATGAGG CGGAGGAGGA AGAGGACAGA GAGAGGAGGA GAGAGATGCA GGAGGAGGAG 
GAGGAGGAGG GATGCAGAGA GAAAGCAGGA GCGCAGGAGC CCATAGACGC ACAGTGCTCC 
TCCTCCATCT CCTCTTCTCC TCTTCATCCC GCCATTCTGG GCAGGAGAGA AGCGCAGAGA 
GAGCGGCTCA ACAAGATCCT GCTCGACCTG CTGCACCGCG CGCCCACTAA GAACGCGATA 
GATGTGACGT ATCTTCTTGA GGAAGGCTCG GGTCGTCGCC TTCGCAGACG GACGCTTGGA 
CTGAGCGAAT TCATTTGCAG AAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159541.1
CDS	765..9731
Translation	MDQRTQGGPSTPPPLPANAPPAEREKEGVGGKKDDEEEKKKREKLRDGAQNEASGAESGTGDQPQQPTQFSVKETSYSEGNVKLKIGLQAKRMKKPPKILENYVCRPAFRATVRHSGGRGGGRANRGAGASDANSNSTSTSNGKEGEKKPSVNRGASQSAAAPAASTPSPSLPPASINTVTPVNGNAPAKRGPPKLACKSEGKADVKSVASSEKPLNLHRPTTESKSHSSGKKNHVPQTNSAPAPSPPAVPPAATSQQDPKIDGILKTPQCNFLEGQKEQERGISSWGTPTVTEKLAQLIATCPPSKCPKPKARKVSPAPAHSSSPPLISNLQRPERAMANRNTYSRAVHLAQPPPVSRPPGRPYGSKNKDSILEKFSGTSKKEESDGLSKGVSGSSSTTTTTTSSALSQNSSRLTTTTSTNESTDINNTDGMKSQSRLGHCPSHSSSAAASPPFSSSSSVERIGSTSCNIGLPGSLTSRPKASPSRIEDFSEKDKERDREKDTDSPRDLSKGITPAITGKQDAKERCSLSAPRNDRGKSSSPVKRSPNQESRVSGVPSPAESTKCTISPSYPSGASRSPPPLCDDTGAISPASPSEQDSKPLKKRRGRRPRWTKAVSRTHKSLHESPSNHQQIPTPMPSGLSFSSPIRESVEQPLSDSYTSPEDSDIFDSSPKKRGRPKTKMPRLDAPAHGCTPNKLAPSKSFSSLLKSKEEQDPPVLHPEVDFNPPKMMARKRGRPKRSPPTLPQETQPPTLAPESGEASVGEKPFRNKGNGQLIMKTIIGKINKMKTVKRKRLLSQILLGPKPAEEQECSRSSGPTGSPVATKPQSLTSLAATFGGKLGPQINVSKKGTIYMGKRRGRKPKTSTNPSPGAPTPPPEPFLSPNTSSPHHHQVQALQNQPHQLPPSEVFPSPSLSQSSGGQSPISDASFVEPGSVHFSSHPHCSHSHQVHHSFSFPPPTFSAPSARSVASAATSTAHPSQKKSSCRGYHHHHHHYRQHYHYRKFSPPRPLLPTSPAPLSELKEATPSPVSESHSEETVPSDSGIGTDNNSTSDRGEKAGSANGLPGVGVSQGMAGGLLMPGVIGPGIGTGIGLSSRGRRRHSSSVLLERPSPTPSPLGVRASPDPRRSHPAAPSSALVGHKEKHKHKCKRRGHGCPSYDKLKRQKRKRKKKYLQLRSRRQDPDFLADLDEICVQLSDIRISHRTPTLRLGSSIGLGAGAGPGRGSGLGSTRPSSSLGGGTNPPPHHYLHRDLLPTIFRINFSGYYSPHPAYPCDSLHYVRKPDLKKKRGRPPKLRESISEVPFVPGLGFPLSSGGFYHPSYGVPYSSTPLGLGYYRGYTPPSALYPHPHHQAPHSGPSHHVHSPSFPPPPPPSYVHHHHPSHLLLNPSKFHKKKHKLLRQEYLGGGRSSMLYPAMSSELSFGWHHKHKHRHKHRDRCGEEEGDDGGGVLGGIGGRGVGGEGLGAGRGERGSVESLQHCRFGRDGSADSRTNKQSASANSPSSSSTSSAERYKRKEASMSCLGPSRLSLGTASRGHHPVDSWFRMGSSDTDYSKLSRNQSFSGSGAFSEGHVQNPVDCSESDEEDAASPIEEPEISHHTNLFASALSRTSLKGSRSKKGGPLESPSLSRLDRTVRRDRSTSSEKREIGNVSSLQNRVGQSSVPDVSEGLHHRHHHHHHPPLSLSHAASCLPHCTLDQPGNTAPLRPSSRQASPSTCSSQPACCLDSPPSHLSHRPPPKNNLLHVNKILRAKKLQRQARTGNNMVKKRGPGRPRKNPLPSPPATPPPQTSEQDNHPLPERHRATALWEADTVVDAIEAVVQNQRKKGRKRKHWERETEGGDEEEEDAEEERQVEDEEEEEDEEQEQQSAAREDDVMSRVRTEGSRQWLTQEEVHCFRSALEGKPDGSPERPGPATSEPTPNPSVTSQREKRVARPPKKKFQKAGLYSDVYKTDDPRSQLLQLKKEKLEYTPGEHDYGLLPAPIHVGKYLRQKRIDFQLPYDILWLWKHDQLYKRPDVPLYKKIRSNVYVDVKPLSGYEATTCNCRLPDDSSEKGCQDDCLNRMIYAECSPSTCPCSDQCDNQRIQKHEWVQCLERFRAEGKGWGIRTKQPLRAGQFIIEYLGEVVSEQEFRKYIASDCRLHQVKIPMLGENKKQIGVSRMMEQYFSHSGHYCLNLDSGMVIDSYRMGNEARFVNHSCEPNCEMQKWSVNGVYRIGLFALKDINSGTELTYDYNFHSFNTEEQQVCKCGSEGCRGIIGGKSKRINGLPAKGAGSGGGARRLGRLKEKRKSKHSLKKREEESSDSSKFYQHLLMKPMSNRERNFVLKHRVFLLRNWEKMREKQELLKREGERERENSGLSLYTRWGGVIRDDGNIKSDVFLTQFSALQTSRSVRTRRLAAAEENTEVTRTARLAHIFKEICDMITSYKDSSGQPLAAPLLNLPSRKRNTQYYEKVTDPLDLSTIDKQILSGHYKTDEAFDADMLKVFRNAEKYYGRKSAVGRDVCRLRKAYYGARHEAAVQIDEIVGETASEADSSDSLERDHAHHHHHDGGGSHDKDDDVIRCICGMYKDEGLMIQCEKCMVWQHCDCMRLEADVEHYLCEQCDPRPVDREVPMVPQPSYAQSGFIYYICLLRDDLLLHQGDCVYLMRDSRRTTEGQPVRQSYRLLSHINRDKLDIFRIEKLWKNEKGERFAFGHHYFRPHETHHSPSRRFYHNELFRVPLYEIIPLEAVVGTCCVLDLYTYCKGRPKGVKEQDVYICDYRLDKSAHLFYKIHRNRYPVCTKPYAFNHFPKRLTPKRDFSPHYVPDNYKRNGGRSAWKSERPKDEEPSSSSTSCERLSSSFPREGDEDGGRGAEGDAEAAQEGSSTPRPQRSSPLESRTAGEEDEAEEEEDRERRREMQEEEEEEGCREKAGAQEPIDAQCSSSISSSPLHPAILGRREAQRERLNKILLDLLHRAPTKNAIDVTYLLEEGSGRRLRRRTLGLSEFICRK

Target ORF information:

RefSeq Version	XM_005159484.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ash1 (absent, small, or homeotic)-like (Drosophila) (ash1l), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159484.4

ORF Insert Sequence:

ATGGATCAGA GGACACAGGG GGGGCCTTCT ACGCCGCCCC CACTCCCTGC CAATGCCCCC 
CCAGCAGAAC GTGAAAAAGA AGGTGTAGGA GGAAAGAAGG ACGATGAAGA AGAGAAAAAG 
AAACGGGAGA AACTAAGAGA TGGGGCACAG AATGAAGCTA GTGGTGCTGA AAGTGGGACT 
GGTGACCAGC CGCAACAACC CACGCAGTTT TCGGTCAAGG AGACCAGCTA CTCTGAGGGT 
AACGTTAAGC TCAAAATAGG CCTGCAGGCC AAACGCATGA AGAAACCTCC TAAGATCCTG 
GAGAACTATG TCTGCCGACC AGCCTTCCGT GCCACTGTAA GGCATAGTGG AGGTAGAGGC 
GGAGGACGTG CTAATCGTGG GGCTGGAGCC AGTGATGCGA ACAGCAACTC TACTAGCACA 
AGTAATGGAA AGGAAGGGGA GAAAAAGCCC TCTGTCAACC GAGGAGCATC TCAGTCAGCT 
GCTGCTCCTG CTGCTTCTAC TCCCTCACCC TCTCTTCCTC CTGCTTCCAT CAACACTGTC 
ACTCCAGTGA ATGGAAATGC TCCAGCAAAA AGGGGTCCTC CAAAACTTGC CTGCAAATCA 
GAGGGAAAGG CTGACGTAAA ATCTGTTGCT TCATCAGAGA AACCTCTTAA TCTCCATCGC 
CCCACGACAG AAAGTAAATC ACACTCCTCT GGAAAGAAAA ACCATGTTCC ACAAACAAAC 
TCTGCCCCTG CCCCATCTCC ACCAGCAGTG CCCCCAGCTG CCACTTCGCA ACAGGACCCT 
AAAATAGATG GAATCTTAAA AACGCCTCAA TGCAATTTTT TGGAGGGGCA GAAGGAACAA 
GAAAGAGGGA TTTCTTCTTG GGGTACCCCT ACAGTAACTG AAAAACTTGC TCAGCTCATA 
GCTACATGCC CACCTTCAAA GTGCCCTAAG CCAAAAGCCC GAAAGGTATC GCCAGCTCCA 
GCACACAGTA GCTCACCTCC GTTAATTTCT AATCTGCAGC GCCCTGAACG GGCAATGGCT 
AATAGAAACA CCTATTCCAG GGCGGTTCAC CTCGCCCAAC CACCACCTGT ATCACGGCCC 
CCTGGTCGCC CATATGGGTC TAAAAACAAA GACAGTATCC TTGAGAAATT TTCTGGAACT 
TCTAAAAAAG AGGAATCAGA TGGACTTTCA AAAGGGGTCT CCGGTAGCAG CAGCACAACC 
ACCACCACCA CTAGTTCAGC ACTGTCTCAA AACAGCAGTC GCTTGACGAC AACAACATCC 
ACAAATGAAA GCACTGACAT TAACAACACA GATGGAATGA AATCTCAGTC TCGTCTTGGG 
CATTGCCCAT CTCATTCTTC ATCCGCAGCT GCTTCACCTC CATTTTCTTC TTCATCCTCC 
GTAGAGAGAA TAGGAAGTAC AAGCTGTAAC ATAGGTTTGC CAGGCTCTCT AACCTCACGC 
CCTAAAGCAA GCCCCTCTCG AATAGAGGAT TTTAGTGAGA AAGACAAAGA ACGTGACCGG 
GAAAAGGACA CAGACAGTCC AAGAGATTTA AGTAAAGGTA TCACTCCAGC TATAACTGGG 
AAGCAGGATG CAAAAGAAAG GTGTTCTTTA TCAGCTCCTC GAAATGACAG GGGTAAGAGC 
TCAAGCCCAG TCAAACGTAG TCCCAATCAG GAAAGTCGCG TTAGTGGGGT ACCAAGTCCT 
GCTGAGTCCA CCAAATGCAC TATTTCTCCT TCATACCCTT CTGGTGCCAG CAGGAGCCCA 
CCACCACTAT GCGATGACAC CGGGGCTATT TCTCCTGCAT CACCATCTGA GCAGGATTCA 
AAACCTCTTA AGAAGCGACG AGGACGACGA CCTCGCTGGA CGAAGGCAGT GAGTCGCACC 
CACAAGTCTT TACATGAGTC TCCTTCTAAC CATCAGCAAA TCCCCACACC AATGCCCTCA 
GGGCTTTCTT TCTCATCCCC TATTCGGGAG TCTGTGGAGC AGCCTCTCAG TGACAGTTAC 
ACATCCCCAG AGGATTCTGA TATTTTCGAT TCTTCGCCCA AGAAGAGAGG ACGTCCAAAG 
ACCAAAATGC CTCGATTAGA TGCACCAGCA CATGGCTGCA CCCCAAACAA GTTGGCACCT 
TCAAAATCCT TCTCCAGTCT GCTCAAGTCT AAGGAAGAGC AGGACCCCCC TGTTTTGCAC 
CCTGAAGTGG ATTTTAATCC ACCTAAAATG ATGGCAAGAA AGCGAGGGCG ACCCAAGCGC 
TCACCTCCAA CTCTTCCTCA AGAAACCCAG CCTCCGACTC TTGCTCCTGA GTCAGGGGAA 
GCATCAGTTG GGGAAAAACC TTTTCGTAAT AAGGGTAATG GGCAGCTCAT TATGAAGACG 
ATCATAGGCA AAATCAATAA AATGAAGACA GTAAAACGGA AGAGGCTCCT CAGCCAAATA 
CTGCTTGGGC CAAAACCAGC AGAGGAGCAG GAATGCTCAA GAAGCAGTGG GCCAACAGGG 
TCTCCGGTTG CTACAAAACC ACAGTCCCTG ACATCACTTG CTGCTACATT TGGTGGCAAA 
TTAGGGCCCC AGATTAATGT AAGCAAAAAG GGGACAATAT ATATGGGTAA AAGAAGGGGT 
CGTAAGCCAA AAACATCAAC TAATCCTTCC CCAGGTGCCC CCACACCCCC TCCTGAGCCT 
TTCTTATCAC CAAACACTTC ATCCCCTCAT CATCACCAGG TGCAGGCTTT ACAAAACCAA 
CCACACCAGT TGCCTCCATC TGAGGTGTTT CCTTCCCCAT CACTATCACA GTCAAGTGGG 
GGCCAAAGCC CCATTAGTGA TGCTAGCTTT GTAGAGCCTG GCTCAGTGCA TTTCTCATCT 
CACCCACACT GCAGTCATTC TCATCAGGTC CACCACTCAT TCTCATTTCC ACCGCCTACT 
TTTTCAGCCC CCTCTGCTCG ATCTGTAGCA AGTGCTGCAA CATCCACTGC ACACCCATCT 
CAGAAGAAGT CCTCTTGCCG GGGTTACCAT CATCACCACC ACCATTACCG GCAGCACTAT 
CATTATCGAA AATTTTCTCC TCCAAGGCCA CTATTGCCAA CGTCTCCAGC TCCACTGAGT 
GAGCTGAAAG AAGCCACACC TTCTCCAGTG AGTGAATCAC ACAGTGAAGA GACTGTACCC 
AGTGACAGTG GCATTGGAAC AGACAATAAT AGCACCTCTG ACCGTGGGGA AAAAGCTGGA 
AGTGCCAATG GCTTACCTGG TGTGGGGGTC TCACAGGGTA TGGCAGGTGG ACTCTTAATG 
CCTGGAGTCA TAGGTCCAGG TATTGGAACG GGAATTGGAC TTTCTTCGAG AGGTCGGAGG 
AGGCACTCAT CTTCAGTTCT TCTAGAACGT CCCTCACCTA CACCCTCACC TCTTGGAGTG 
AGAGCATCAC CTGACCCAAG AAGATCGCAC CCTGCTGCCC CATCTTCTGC TTTGGTTGGA 
CATAAAGAGA AACACAAGCA TAAGTGCAAG CGTCGTGGGC ATGGCTGCCC TAGCTATGAC 
AAACTAAAAA GGCAAAAGCG TAAACGCAAG AAGAAGTACC TGCAGCTACG GTCCCGCAGA 
CAAGACCCAG ATTTTTTGGC TGATTTAGAT GAGATATGTG TGCAGCTTAG TGATATACGA 
ATTTCTCATC GAACACCTAC ACTTCGTTTG GGCAGCAGCA TAGGTTTGGG TGCCGGGGCT 
GGTCCTGGCC GTGGGTCTGG CTTGGGAAGT ACCAGACCTA GCAGCAGCCT TGGAGGCGGA 
ACCAATCCAC CACCCCATCA CTATCTGCAT AGAGATCTAT TACCCACAAT ATTCAGGATA 
AATTTTAGTG GGTATTATTC ACCCCACCCA GCCTACCCAT GTGATTCACT TCATTACGTT 
CGTAAGCCAG ATCTTAAAAA GAAAAGAGGT CGACCACCTA AGCTTCGGGA ATCCATATCA 
GAAGTACCTT TTGTACCTGG TTTAGGCTTT CCCCTCTCAA GTGGAGGTTT CTATCACCCT 
TCTTATGGTG TCCCATACTC TTCTACCCCA CTGGGACTTG GTTACTATCG TGGTTATACC 
CCACCAAGTG CCCTGTACCC CCATCCTCAT CACCAGGCTC CCCACTCTGG CCCATCCCAC 
CATGTTCATT CACCATCTTT TCCTCCACCT CCACCCCCTT CTTATGTGCA TCATCATCAC 
CCATCCCACC TGCTTCTTAA CCCCTCCAAA TTTCACAAGA AGAAACATAA GCTACTTAGG 
CAAGAGTATT TAGGTGGTGG TCGTTCATCC ATGCTCTATC CTGCCATGTC ATCTGAGCTG 
TCATTTGGCT GGCACCACAA ACACAAGCAT AGGCACAAAC ACCGTGATCG CTGTGGAGAA 
GAGGAGGGTG ATGATGGTGG TGGAGTTTTA GGTGGTATAG GAGGCAGAGG TGTTGGAGGT 
GAGGGCTTGG GGGCCGGAAG AGGGGAGAGA GGGAGCGTAG AGTCCCTGCA GCACTGCCGG 
TTTGGACGAG ATGGCTCTGC TGACTCCCGA ACTAACAAAC AGTCAGCTTC TGCCAACTCC 
CCTTCCTCTT CCTCCACATC CTCTGCAGAA CGGTACAAGC GAAAAGAGGC CTCTATGTCT 
TGTCTTGGGC CCTCACGACT GTCTTTGGGC ACTGCAAGTC GGGGACATCA TCCTGTTGAC 
TCCTGGTTCA GAATGGGAAG TTCTGATACT GATTACTCCA AGCTCTCTAG AAACCAGTCT 
TTTTCGGGGT CTGGTGCATT CTCTGAAGGA CATGTGCAAA ATCCAGTGGA TTGCTCAGAG 
AGTGATGAGG AAGATGCTGC TTCCCCCATT GAAGAGCCAG AGATCAGCCA TCACACAAAC 
CTGTTTGCAT CTGCACTATC AAGGACCTCC CTCAAAGGAA GTCGCAGCAA AAAAGGTGGA 
CCATTAGAGA GTCCCAGTCT CTCACGGTTG GACAGGACGG TTAGAAGGGA TCGTTCAACA 
TCTTCAGAGA AAAGAGAAAT TGGGAATGTA TCTAGCTTGC AGAACAGAGT TGGCCAATCC 
AGTGTTCCTG ATGTTTCTGA GGGTCTCCAT CATCGGCACC ACCATCACCA CCACCCTCCT 
CTATCCCTCT CGCACGCTGC CTCTTGCCTG CCGCACTGCA CTCTAGACCA GCCTGGAAAC 
ACTGCACCAC TCCGGCCCTC CAGCCGCCAA GCTAGTCCAT CCACATGCTC CTCCCAGCCT 
GCATGCTGCC TTGATTCCCC ACCTTCACAT CTCTCCCACC GGCCCCCTCC CAAAAACAAC 
CTGCTTCATG TCAACAAGAT CCTCCGTGCA AAGAAGCTCC AAAGGCAAGC ACGTACAGGG 
AACAACATGG TGAAAAAACG GGGTCCAGGA CGTCCCCGCA AGAACCCGCT GCCCTCACCT 
CCTGCAACAC CCCCACCACA GACTTCCGAA CAAGACAACC ACCCATTACC TGAAAGGCAC 
CGAGCAACTG CACTCTGGGA GGCAGATACT GTAGTTGATG CCATTGAGGC TGTCGTACAG 
AACCAGCGCA AAAAGGGACG CAAGCGGAAG CACTGGGAAA GGGAGACTGA AGGAGGTGAT 
GAAGAAGAGG AGGATGCGGA GGAAGAAAGA CAGGTGGAGG ACGAAGAAGA AGAAGAAGAC 
GAAGAACAAG AACAACAGTC AGCTGCTAGA GAAGACGATG TAATGTCGAG AGTTAGAACA 
GAAGGAAGCA GGCAATGGTT GACCCAGGAA GAAGTGCACT GTTTTCGCAG TGCACTGGAG 
GGAAAACCTG ATGGCTCCCC TGAGCGCCCA GGCCCTGCTA CTTCGGAACC AACCCCAAAC 
CCATCAGTGA CCAGCCAACG TGAGAAGAGA GTTGCTAGAC CACCCAAAAA GAAGTTTCAA 
AAGGCGGGAC TTTACTCTGA TGTGTACAAG ACTGATGATC CTCGCAGTCA GCTGCTACAG 
TTAAAGAAAG AGAAGCTAGA ATACACTCCA GGTGAACATG ACTATGGCCT TCTTCCTGCT 
CCCATTCATG TTGGGAAATA CTTGAGACAG AAACGCATTG ATTTCCAGCT GCCTTACGAC 
ATCCTGTGGC TTTGGAAACA TGATCAGCTC TACAAGAGAC CTGACGTGCC TCTCTACAAG 
AAGATCAGAT CCAATGTCTA TGTGGATGTG AAGCCTCTTT CTGGCTATGA AGCCACTACC 
TGTAACTGCA GGTTGCCTGA CGACTCTAGT GAAAAGGGCT GTCAAGATGA TTGTCTAAAC 
AGGATGATTT ATGCCGAGTG CTCTCCAAGT ACGTGTCCGT GTTCCGATCA GTGCGATAAC 
CAGCGGATCC AGAAGCATGA GTGGGTTCAG TGCCTCGAGC GCTTCCGAGC TGAGGGAAAG 
GGCTGGGGCA TCCGGACCAA GCAGCCCCTT CGTGCCGGCC AATTTATCAT CGAGTACTTG 
GGAGAGGTGG TCAGCGAACA AGAGTTCAGA AAATACATTG CGTCTGACTG TAGGTTGCAC 
CAAGTCAAAA TTCCAATGTT GGGGGAAAAT AAAAAGCAGA TTGGTGTGAG TCGCATGATG 
GAGCAGTACT TCTCTCACAG CGGCCACTAC TGCCTGAATC TGGACAGCGG CATGGTCATC 
GATAGCTACC GCATGGGCAA CGAGGCTCGC TTTGTCAACC ACAGCTGCGA GCCCAACTGT 
GAGATGCAGA AATGGTCGGT GAACGGAGTG TACCGAATTG GCCTGTTTGC TCTAAAAGAC 
ATCAACAGCG GAACGGAACT GACATATGAC TACAACTTCC ACTCCTTCAA CACAGAGGAA 
CAACAAGTCT GCAAGTGTGG CTCAGAGGGT TGCCGAGGCA TCATTGGCGG CAAGAGCAAG 
CGAATCAATG GGCTGCCTGC TAAAGGGGCC GGATCTGGAG GCGGAGCCCG GAGACTTGGA 
AGATTGAAAG AGAAACGCAA GTCCAAACAC TCACTGAAGA AGCGGGAGGA AGAGTCCAGT 
GACAGCAGCA AGTTTTACCA GCACCTTTTA ATGAAGCCCA TGTCAAACAG AGAGAGGAAC 
TTTGTGCTGA AACATCGGGT CTTTCTGCTG CGGAATTGGG AGAAGATGCG GGAGAAACAG 
GAGCTTCTGA AGCGTGAGGG AGAGCGGGAG AGGGAGAACA GCGGTCTGTC TCTGTACACC 
CGCTGGGGAG GAGTCATCCG GGATGATGGA AACATCAAAT CAGATGTATT TCTGACTCAG 
TTCTCAGCGC TGCAGACGTC CCGCTCTGTA CGCACACGCA GACTTGCGGC AGCCGAGGAG 
AACACTGAAG TGACACGCAC CGCACGTCTG GCACACATTT TCAAAGAGAT CTGCGATATG 
ATCACTAGCT ACAAGGATTC ATCTGGTCAG CCTCTTGCTG CTCCGCTGCT TAACCTGCCT 
TCTAGGAAGA GGAACACACA ATACTATGAG AAGGTCACCG ATCCTCTAGA TCTCAGCACC 
ATAGATAAGC AGATCCTCAG CGGACACTAC AAAACCGATG AGGCGTTTGA TGCGGACATG 
CTCAAAGTGT TTCGCAATGC TGAGAAGTAC TATGGGCGTA AGTCTGCTGT GGGCCGTGAC 
GTGTGCCGGC TGAGGAAGGC TTATTATGGT GCCCGTCATG AGGCTGCTGT TCAGATCGAT 
GAGATTGTGG GTGAGACGGC AAGTGAAGCG GACAGCTCTG ATTCGCTGGA GAGAGACCAC 
GCCCACCACC ATCACCATGA CGGAGGGGGC AGCCATGATA AGGATGATGA TGTGATCCGC 
TGCATCTGTG GCATGTACAA GGATGAGGGA CTCATGATTC AGTGCGAGAA GTGCATGGTG 
TGGCAGCACT GTGATTGTAT GCGTCTGGAG GCGGATGTGG AGCATTATCT CTGTGAACAG 
TGTGACCCTC GTCCAGTGGA CAGAGAGGTT CCAATGGTGC CTCAACCCAG CTATGCCCAA 
TCAGGATTCA TCTATTACAT ATGCCTGCTT CGCGATGACT TACTGTTGCA TCAGGGCGAT 
TGTGTGTACC TCATGCGGGA CAGCAGACGC ACAACAGAAG GTCAGCCGGT GCGTCAGTCA 
TACCGCCTCC TGTCTCACAT CAACAGAGAC AAACTGGACA TCTTCCGTAT CGAGAAACTC 
TGGAAGAATG AAAAAGGTGA GAGGTTTGCA TTTGGTCATC ATTACTTCCG TCCTCATGAG 
ACGCATCACT CCCCTTCCCG CCGCTTCTAC CACAACGAGC TGTTTCGAGT GCCTCTGTAT 
GAGATCATCC CGCTGGAGGC CGTGGTGGGC ACCTGCTGCG TGCTGGACCT CTACACATAC 
TGCAAGGGTC GTCCAAAAGG GGTAAAGGAG CAGGACGTTT ACATCTGCGA CTACCGGCTG 
GATAAATCCG CACACCTGTT CTACAAGATC CACCGCAACC GTTACCCAGT GTGCACCAAG 
CCCTATGCGT TCAACCACTT CCCCAAGAGA CTGACGCCCA AGAGGGACTT CTCTCCTCAC 
TATGTTCCAG ATAACTACAA GAGGAACGGC GGACGCTCTG CTTGGAAGAG CGAGCGGCCC 
AAAGATGAAG AACCATCATC CTCCAGCACG TCCTGCGAGC GTCTGTCCTC CAGCTTCCCT 
AGAGAAGGAG ACGAGGACGG AGGACGAGGA GCAGAAGGAG ACGCGGAGGC CGCTCAAGAG 
GGATCCAGCA CTCCGAGACC TCAGAGATCC TCGCCACTGG AGAGCAGGAC GGCCGGAGAG 
GAGGATGAGG CGGAGGAGGA AGAGGACAGA GAGAGGAGGA GAGAGATGCA GGAGGAGGAG 
GAGGAGGAGG GATGCAGAGA GAAAGCAGGA GCGCAGGAGC CCATAGACGC ACAGTGCTCC 
TCCTCCATCT CCTCTTCTCC TCTTCATCCC GCCATTCTGG GCAGGAGAGA AGCGCAGAGA 
GAGCGGCTCA ACAAGATCCT GCTCGACCTG CTGCACCGCG CGCCCACTAA GAACGCGATA 
GATGTGACGT ATCTTCTTGA GGAAGGCTCG GGTCGTCGCC TTCGCAGACG GACGCTTGGA 
CTGAGCGAAT TCATTTGCAG AAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ash1l ( XM_005159483.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159540.1 Danio rerio ash1 (absent, small, or homeotic)-like (Drosophila) (ash1l), transcript variant X1, mRNA.

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CloneID	ODa23935
Clone ID Related Accession (Same CDS sequence)	XM_005159483.3 , XM_005159484.3 , XM_005159484.4 , XM_005159483.4
Accession Version	XM_005159483.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8967bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	probable histone-lysine N-methyltransferase ASH1L isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159483.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCAGA GGACACAGGG GGGGCCTTCT ACGCCGCCCC CACTCCCTGC CAATGCCCCC 
CCAGCAGAAC GTGAAAAAGA AGGTGTAGGA GGAAAGAAGG ACGATGAAGA AGAGAAAAAG 
AAACGGGAGA AACTAAGAGA TGGGGCACAG AATGAAGCTA GTGGTGCTGA AAGTGGGACT 
GGTGACCAGC CGCAACAACC CACGCAGTTT TCGGTCAAGG AGACCAGCTA CTCTGAGGGT 
AACGTTAAGC TCAAAATAGG CCTGCAGGCC AAACGCATGA AGAAACCTCC TAAGATCCTG 
GAGAACTATG TCTGCCGACC AGCCTTCCGT GCCACTGTAA GGCATAGTGG AGGTAGAGGC 
GGAGGACGTG CTAATCGTGG GGCTGGAGCC AGTGATGCGA ACAGCAACTC TACTAGCACA 
AGTAATGGAA AGGAAGGGGA GAAAAAGCCC TCTGTCAACC GAGGAGCATC TCAGTCAGCT 
GCTGCTCCTG CTGCTTCTAC TCCCTCACCC TCTCTTCCTC CTGCTTCCAT CAACACTGTC 
ACTCCAGTGA ATGGAAATGC TCCAGCAAAA AGGGGTCCTC CAAAACTTGC CTGCAAATCA 
GAGGGAAAGG CTGACGTAAA ATCTGTTGCT TCATCAGAGA AACCTCTTAA TCTCCATCGC 
CCCACGACAG AAAGTAAATC ACACTCCTCT GGAAAGAAAA ACCATGTTCC ACAAACAAAC 
TCTGCCCCTG CCCCATCTCC ACCAGCAGTG CCCCCAGCTG CCACTTCGCA ACAGGACCCT 
AAAATAGATG GAATCTTAAA AACGCCTCAA TGCAATTTTT TGGAGGGGCA GAAGGAACAA 
GAAAGAGGGA TTTCTTCTTG GGGTACCCCT ACAGTAACTG AAAAACTTGC TCAGCTCATA 
GCTACATGCC CACCTTCAAA GTGCCCTAAG CCAAAAGCCC GAAAGGTATC GCCAGCTCCA 
GCACACAGTA GCTCACCTCC GTTAATTTCT AATCTGCAGC GCCCTGAACG GGCAATGGCT 
AATAGAAACA CCTATTCCAG GGCGGTTCAC CTCGCCCAAC CACCACCTGT ATCACGGCCC 
CCTGGTCGCC CATATGGGTC TAAAAACAAA GACAGTATCC TTGAGAAATT TTCTGGAACT 
TCTAAAAAAG AGGAATCAGA TGGACTTTCA AAAGGGGTCT CCGGTAGCAG CAGCACAACC 
ACCACCACCA CTAGTTCAGC ACTGTCTCAA AACAGCAGTC GCTTGACGAC AACAACATCC 
ACAAATGAAA GCACTGACAT TAACAACACA GATGGAATGA AATCTCAGTC TCGTCTTGGG 
CATTGCCCAT CTCATTCTTC ATCCGCAGCT GCTTCACCTC CATTTTCTTC TTCATCCTCC 
GTAGAGAGAA TAGGAAGTAC AAGCTGTAAC ATAGGTTTGC CAGGCTCTCT AACCTCACGC 
CCTAAAGCAA GCCCCTCTCG AATAGAGGAT TTTAGTGAGA AAGACAAAGA ACGTGACCGG 
GAAAAGGACA CAGACAGTCC AAGAGATTTA AGTAAAGGTA TCACTCCAGC TATAACTGGG 
AAGCAGGATG CAAAAGAAAG GTGTTCTTTA TCAGCTCCTC GAAATGACAG GGGTAAGAGC 
TCAAGCCCAG TCAAACGTAG TCCCAATCAG GAAAGTCGCG TTAGTGGGGT ACCAAGTCCT 
GCTGAGTCCA CCAAATGCAC TATTTCTCCT TCATACCCTT CTGGTGCCAG CAGGAGCCCA 
CCACCACTAT GCGATGACAC CGGGGCTATT TCTCCTGCAT CACCATCTGA GCAGGATTCA 
AAACCTCTTA AGAAGCGACG AGGACGACGA CCTCGCTGGA CGAAGGCAGT GAGTCGCACC 
CACAAGTCTT TACATGAGTC TCCTTCTAAC CATCAGCAAA TCCCCACACC AATGCCCTCA 
GGGCTTTCTT TCTCATCCCC TATTCGGGAG TCTGTGGAGC AGCCTCTCAG TGACAGTTAC 
ACATCCCCAG AGGATTCTGA TATTTTCGAT TCTTCGCCCA AGAAGAGAGG ACGTCCAAAG 
ACCAAAATGC CTCGATTAGA TGCACCAGCA CATGGCTGCA CCCCAAACAA GTTGGCACCT 
TCAAAATCCT TCTCCAGTCT GCTCAAGTCT AAGGAAGAGC AGGACCCCCC TGTTTTGCAC 
CCTGAAGTGG ATTTTAATCC ACCTAAAATG ATGGCAAGAA AGCGAGGGCG ACCCAAGCGC 
TCACCTCCAA CTCTTCCTCA AGAAACCCAG CCTCCGACTC TTGCTCCTGA GTCAGGGGAA 
GCATCAGTTG GGGAAAAACC TTTTCGTAAT AAGGGTAATG GGCAGCTCAT TATGAAGACG 
ATCATAGGCA AAATCAATAA AATGAAGACA GTAAAACGGA AGAGGCTCCT CAGCCAAATA 
CTGCTTGGGC CAAAACCAGC AGAGGAGCAG GAATGCTCAA GAAGCAGTGG GCCAACAGGG 
TCTCCGGTTG CTACAAAACC ACAGTCCCTG ACATCACTTG CTGCTACATT TGGTGGCAAA 
TTAGGGCCCC AGATTAATGT AAGCAAAAAG GGGACAATAT ATATGGGTAA AAGAAGGGGT 
CGTAAGCCAA AAACATCAAC TAATCCTTCC CCAGGTGCCC CCACACCCCC TCCTGAGCCT 
TTCTTATCAC CAAACACTTC ATCCCCTCAT CATCACCAGG TGCAGGCTTT ACAAAACCAA 
CCACACCAGT TGCCTCCATC TGAGGTGTTT CCTTCCCCAT CACTATCACA GTCAAGTGGG 
GGCCAAAGCC CCATTAGTGA TGCTAGCTTT GTAGAGCCTG GCTCAGTGCA TTTCTCATCT 
CACCCACACT GCAGTCATTC TCATCAGGTC CACCACTCAT TCTCATTTCC ACCGCCTACT 
TTTTCAGCCC CCTCTGCTCG ATCTGTAGCA AGTGCTGCAA CATCCACTGC ACACCCATCT 
CAGAAGAAGT CCTCTTGCCG GGGTTACCAT CATCACCACC ACCATTACCG GCAGCACTAT 
CATTATCGAA AATTTTCTCC TCCAAGGCCA CTATTGCCAA CGTCTCCAGC TCCACTGAGT 
GAGCTGAAAG AAGCCACACC TTCTCCAGTG AGTGAATCAC ACAGTGAAGA GACTGTACCC 
AGTGACAGTG GCATTGGAAC AGACAATAAT AGCACCTCTG ACCGTGGGGA AAAAGCTGGA 
AGTGCCAATG GCTTACCTGG TGTGGGGGTC TCACAGGGTA TGGCAGGTGG ACTCTTAATG 
CCTGGAGTCA TAGGTCCAGG TATTGGAACG GGAATTGGAC TTTCTTCGAG AGGTCGGAGG 
AGGCACTCAT CTTCAGTTCT TCTAGAACGT CCCTCACCTA CACCCTCACC TCTTGGAGTG 
AGAGCATCAC CTGACCCAAG AAGATCGCAC CCTGCTGCCC CATCTTCTGC TTTGGTTGGA 
CATAAAGAGA AACACAAGCA TAAGTGCAAG CGTCGTGGGC ATGGCTGCCC TAGCTATGAC 
AAACTAAAAA GGCAAAAGCG TAAACGCAAG AAGAAGTACC TGCAGCTACG GTCCCGCAGA 
CAAGACCCAG ATTTTTTGGC TGATTTAGAT GAGATATGTG TGCAGCTTAG TGATATACGA 
ATTTCTCATC GAACACCTAC ACTTCGTTTG GGCAGCAGCA TAGGTTTGGG TGCCGGGGCT 
GGTCCTGGCC GTGGGTCTGG CTTGGGAAGT ACCAGACCTA GCAGCAGCCT TGGAGGCGGA 
ACCAATCCAC CACCCCATCA CTATCTGCAT AGAGATCTAT TACCCACAAT ATTCAGGATA 
AATTTTAGTG GGTATTATTC ACCCCACCCA GCCTACCCAT GTGATTCACT TCATTACGTT 
CGTAAGCCAG ATCTTAAAAA GAAAAGAGGT CGACCACCTA AGCTTCGGGA ATCCATATCA 
GAAGTACCTT TTGTACCTGG TTTAGGCTTT CCCCTCTCAA GTGGAGGTTT CTATCACCCT 
TCTTATGGTG TCCCATACTC TTCTACCCCA CTGGGACTTG GTTACTATCG TGGTTATACC 
CCACCAAGTG CCCTGTACCC CCATCCTCAT CACCAGGCTC CCCACTCTGG CCCATCCCAC 
CATGTTCATT CACCATCTTT TCCTCCACCT CCACCCCCTT CTTATGTGCA TCATCATCAC 
CCATCCCACC TGCTTCTTAA CCCCTCCAAA TTTCACAAGA AGAAACATAA GCTACTTAGG 
CAAGAGTATT TAGGTGGTGG TCGTTCATCC ATGCTCTATC CTGCCATGTC ATCTGAGCTG 
TCATTTGGCT GGCACCACAA ACACAAGCAT AGGCACAAAC ACCGTGATCG CTGTGGAGAA 
GAGGAGGGTG ATGATGGTGG TGGAGTTTTA GGTGGTATAG GAGGCAGAGG TGTTGGAGGT 
GAGGGCTTGG GGGCCGGAAG AGGGGAGAGA GGGAGCGTAG AGTCCCTGCA GCACTGCCGG 
TTTGGACGAG ATGGCTCTGC TGACTCCCGA ACTAACAAAC AGTCAGCTTC TGCCAACTCC 
CCTTCCTCTT CCTCCACATC CTCTGCAGAA CGGTACAAGC GAAAAGAGGC CTCTATGTCT 
TGTCTTGGGC CCTCACGACT GTCTTTGGGC ACTGCAAGTC GGGGACATCA TCCTGTTGAC 
TCCTGGTTCA GAATGGGAAG TTCTGATACT GATTACTCCA AGCTCTCTAG AAACCAGTCT 
TTTTCGGGGT CTGGTGCATT CTCTGAAGGA CATGTGCAAA ATCCAGTGGA TTGCTCAGAG 
AGTGATGAGG AAGATGCTGC TTCCCCCATT GAAGAGCCAG AGATCAGCCA TCACACAAAC 
CTGTTTGCAT CTGCACTATC AAGGACCTCC CTCAAAGGAA GTCGCAGCAA AAAAGGTGGA 
CCATTAGAGA GTCCCAGTCT CTCACGGTTG GACAGGACGG TTAGAAGGGA TCGTTCAACA 
TCTTCAGAGA AAAGAGAAAT TGGGAATGTA TCTAGCTTGC AGAACAGAGT TGGCCAATCC 
AGTGTTCCTG ATGTTTCTGA GGGTCTCCAT CATCGGCACC ACCATCACCA CCACCCTCCT 
CTATCCCTCT CGCACGCTGC CTCTTGCCTG CCGCACTGCA CTCTAGACCA GCCTGGAAAC 
ACTGCACCAC TCCGGCCCTC CAGCCGCCAA GCTAGTCCAT CCACATGCTC CTCCCAGCCT 
GCATGCTGCC TTGATTCCCC ACCTTCACAT CTCTCCCACC GGCCCCCTCC CAAAAACAAC 
CTGCTTCATG TCAACAAGAT CCTCCGTGCA AAGAAGCTCC AAAGGCAAGC ACGTACAGGG 
AACAACATGG TGAAAAAACG GGGTCCAGGA CGTCCCCGCA AGAACCCGCT GCCCTCACCT 
CCTGCAACAC CCCCACCACA GACTTCCGAA CAAGACAACC ACCCATTACC TGAAAGGCAC 
CGAGCAACTG CACTCTGGGA GGCAGATACT GTAGTTGATG CCATTGAGGC TGTCGTACAG 
AACCAGCGCA AAAAGGGACG CAAGCGGAAG CACTGGGAAA GGGAGACTGA AGGAGGTGAT 
GAAGAAGAGG AGGATGCGGA GGAAGAAAGA CAGGTGGAGG ACGAAGAAGA AGAAGAAGAC 
GAAGAACAAG AACAACAGTC AGCTGCTAGA GAAGACGATG TAATGTCGAG AGTTAGAACA 
GAAGGAAGCA GGCAATGGTT GACCCAGGAA GAAGTGCACT GTTTTCGCAG TGCACTGGAG 
GGAAAACCTG ATGGCTCCCC TGAGCGCCCA GGCCCTGCTA CTTCGGAACC AACCCCAAAC 
CCATCAGTGA CCAGCCAACG TGAGAAGAGA GTTGCTAGAC CACCCAAAAA GAAGTTTCAA 
AAGGCGGGAC TTTACTCTGA TGTGTACAAG ACTGATGATC CTCGCAGTCA GCTGCTACAG 
TTAAAGAAAG AGAAGCTAGA ATACACTCCA GGTGAACATG ACTATGGCCT TCTTCCTGCT 
CCCATTCATG TTGGGAAATA CTTGAGACAG AAACGCATTG ATTTCCAGCT GCCTTACGAC 
ATCCTGTGGC TTTGGAAACA TGATCAGCTC TACAAGAGAC CTGACGTGCC TCTCTACAAG 
AAGATCAGAT CCAATGTCTA TGTGGATGTG AAGCCTCTTT CTGGCTATGA AGCCACTACC 
TGTAACTGCA GGTTGCCTGA CGACTCTAGT GAAAAGGGCT GTCAAGATGA TTGTCTAAAC 
AGGATGATTT ATGCCGAGTG CTCTCCAAGT ACGTGTCCGT GTTCCGATCA GTGCGATAAC 
CAGCGGATCC AGAAGCATGA GTGGGTTCAG TGCCTCGAGC GCTTCCGAGC TGAGGGAAAG 
GGCTGGGGCA TCCGGACCAA GCAGCCCCTT CGTGCCGGCC AATTTATCAT CGAGTACTTG 
GGAGAGGTGG TCAGCGAACA AGAGTTCAGA AAATACATTG CGTCTGACTG TAGGTTGCAC 
CAAGTCAAAA TTCCAATGTT GGGGGAAAAT AAAAAGCAGA TTGGTGTGAG TCGCATGATG 
GAGCAGTACT TCTCTCACAG CGGCCACTAC TGCCTGAATC TGGACAGCGG CATGGTCATC 
GATAGCTACC GCATGGGCAA CGAGGCTCGC TTTGTCAACC ACAGCTGCGA GCCCAACTGT 
GAGATGCAGA AATGGTCGGT GAACGGAGTG TACCGAATTG GCCTGTTTGC TCTAAAAGAC 
ATCAACAGCG GAACGGAACT GACATATGAC TACAACTTCC ACTCCTTCAA CACAGAGGAA 
CAACAAGTCT GCAAGTGTGG CTCAGAGGGT TGCCGAGGCA TCATTGGCGG CAAGAGCAAG 
CGAATCAATG GGCTGCCTGC TAAAGGGGCC GGATCTGGAG GCGGAGCCCG GAGACTTGGA 
AGATTGAAAG AGAAACGCAA GTCCAAACAC TCACTGAAGA AGCGGGAGGA AGAGTCCAGT 
GACAGCAGCA AGTTTTACCA GCACCTTTTA ATGAAGCCCA TGTCAAACAG AGAGAGGAAC 
TTTGTGCTGA AACATCGGGT CTTTCTGCTG CGGAATTGGG AGAAGATGCG GGAGAAACAG 
GAGCTTCTGA AGCGTGAGGG AGAGCGGGAG AGGGAGAACA GCGGTCTGTC TCTGTACACC 
CGCTGGGGAG GAGTCATCCG GGATGATGGA AACATCAAAT CAGATGTATT TCTGACTCAG 
TTCTCAGCGC TGCAGACGTC CCGCTCTGTA CGCACACGCA GACTTGCGGC AGCCGAGGAG 
AACACTGAAG TGACACGCAC CGCACGTCTG GCACACATTT TCAAAGAGAT CTGCGATATG 
ATCACTAGCT ACAAGGATTC ATCTGGTCAG CCTCTTGCTG CTCCGCTGCT TAACCTGCCT 
TCTAGGAAGA GGAACACACA ATACTATGAG AAGGTCACCG ATCCTCTAGA TCTCAGCACC 
ATAGATAAGC AGATCCTCAG CGGACACTAC AAAACCGATG AGGCGTTTGA TGCGGACATG 
CTCAAAGTGT TTCGCAATGC TGAGAAGTAC TATGGGCGTA AGTCTGCTGT GGGCCGTGAC 
GTGTGCCGGC TGAGGAAGGC TTATTATGGT GCCCGTCATG AGGCTGCTGT TCAGATCGAT 
GAGATTGTGG GTGAGACGGC AAGTGAAGCG GACAGCTCTG ATTCGCTGGA GAGAGACCAC 
GCCCACCACC ATCACCATGA CGGAGGGGGC AGCCATGATA AGGATGATGA TGTGATCCGC 
TGCATCTGTG GCATGTACAA GGATGAGGGA CTCATGATTC AGTGCGAGAA GTGCATGGTG 
TGGCAGCACT GTGATTGTAT GCGTCTGGAG GCGGATGTGG AGCATTATCT CTGTGAACAG 
TGTGACCCTC GTCCAGTGGA CAGAGAGGTT CCAATGGTGC CTCAACCCAG CTATGCCCAA 
TCAGGATTCA TCTATTACAT ATGCCTGCTT CGCGATGACT TACTGTTGCA TCAGGGCGAT 
TGTGTGTACC TCATGCGGGA CAGCAGACGC ACAACAGAAG GTCAGCCGGT GCGTCAGTCA 
TACCGCCTCC TGTCTCACAT CAACAGAGAC AAACTGGACA TCTTCCGTAT CGAGAAACTC 
TGGAAGAATG AAAAAGGTGA GAGGTTTGCA TTTGGTCATC ATTACTTCCG TCCTCATGAG 
ACGCATCACT CCCCTTCCCG CCGCTTCTAC CACAACGAGC TGTTTCGAGT GCCTCTGTAT 
GAGATCATCC CGCTGGAGGC CGTGGTGGGC ACCTGCTGCG TGCTGGACCT CTACACATAC 
TGCAAGGGTC GTCCAAAAGG GGTAAAGGAG CAGGACGTTT ACATCTGCGA CTACCGGCTG 
GATAAATCCG CACACCTGTT CTACAAGATC CACCGCAACC GTTACCCAGT GTGCACCAAG 
CCCTATGCGT TCAACCACTT CCCCAAGAGA CTGACGCCCA AGAGGGACTT CTCTCCTCAC 
TATGTTCCAG ATAACTACAA GAGGAACGGC GGACGCTCTG CTTGGAAGAG CGAGCGGCCC 
AAAGATGAAG AACCATCATC CTCCAGCACG TCCTGCGAGC GTCTGTCCTC CAGCTTCCCT 
AGAGAAGGAG ACGAGGACGG AGGACGAGGA GCAGAAGGAG ACGCGGAGGC CGCTCAAGAG 
GGATCCAGCA CTCCGAGACC TCAGAGATCC TCGCCACTGG AGAGCAGGAC GGCCGGAGAG 
GAGGATGAGG CGGAGGAGGA AGAGGACAGA GAGAGGAGGA GAGAGATGCA GGAGGAGGAG 
GAGGAGGAGG GATGCAGAGA GAAAGCAGGA GCGCAGGAGC CCATAGACGC ACAGTGCTCC 
TCCTCCATCT CCTCTTCTCC TCTTCATCCC GCCATTCTGG GCAGGAGAGA AGCGCAGAGA 
GAGCGGCTCA ACAAGATCCT GCTCGACCTG CTGCACCGCG CGCCCACTAA GAACGCGATA 
GATGTGACGT ATCTTCTTGA GGAAGGCTCG GGTCGTCGCC TTCGCAGACG GACGCTTGGA 
CTGAGCGAAT TCATTTGCAG AAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159540.1
CDS	463..9429
Translation	MDQRTQGGPSTPPPLPANAPPAEREKEGVGGKKDDEEEKKKREKLRDGAQNEASGAESGTGDQPQQPTQFSVKETSYSEGNVKLKIGLQAKRMKKPPKILENYVCRPAFRATVRHSGGRGGGRANRGAGASDANSNSTSTSNGKEGEKKPSVNRGASQSAAAPAASTPSPSLPPASINTVTPVNGNAPAKRGPPKLACKSEGKADVKSVASSEKPLNLHRPTTESKSHSSGKKNHVPQTNSAPAPSPPAVPPAATSQQDPKIDGILKTPQCNFLEGQKEQERGISSWGTPTVTEKLAQLIATCPPSKCPKPKARKVSPAPAHSSSPPLISNLQRPERAMANRNTYSRAVHLAQPPPVSRPPGRPYGSKNKDSILEKFSGTSKKEESDGLSKGVSGSSSTTTTTTSSALSQNSSRLTTTTSTNESTDINNTDGMKSQSRLGHCPSHSSSAAASPPFSSSSSVERIGSTSCNIGLPGSLTSRPKASPSRIEDFSEKDKERDREKDTDSPRDLSKGITPAITGKQDAKERCSLSAPRNDRGKSSSPVKRSPNQESRVSGVPSPAESTKCTISPSYPSGASRSPPPLCDDTGAISPASPSEQDSKPLKKRRGRRPRWTKAVSRTHKSLHESPSNHQQIPTPMPSGLSFSSPIRESVEQPLSDSYTSPEDSDIFDSSPKKRGRPKTKMPRLDAPAHGCTPNKLAPSKSFSSLLKSKEEQDPPVLHPEVDFNPPKMMARKRGRPKRSPPTLPQETQPPTLAPESGEASVGEKPFRNKGNGQLIMKTIIGKINKMKTVKRKRLLSQILLGPKPAEEQECSRSSGPTGSPVATKPQSLTSLAATFGGKLGPQINVSKKGTIYMGKRRGRKPKTSTNPSPGAPTPPPEPFLSPNTSSPHHHQVQALQNQPHQLPPSEVFPSPSLSQSSGGQSPISDASFVEPGSVHFSSHPHCSHSHQVHHSFSFPPPTFSAPSARSVASAATSTAHPSQKKSSCRGYHHHHHHYRQHYHYRKFSPPRPLLPTSPAPLSELKEATPSPVSESHSEETVPSDSGIGTDNNSTSDRGEKAGSANGLPGVGVSQGMAGGLLMPGVIGPGIGTGIGLSSRGRRRHSSSVLLERPSPTPSPLGVRASPDPRRSHPAAPSSALVGHKEKHKHKCKRRGHGCPSYDKLKRQKRKRKKKYLQLRSRRQDPDFLADLDEICVQLSDIRISHRTPTLRLGSSIGLGAGAGPGRGSGLGSTRPSSSLGGGTNPPPHHYLHRDLLPTIFRINFSGYYSPHPAYPCDSLHYVRKPDLKKKRGRPPKLRESISEVPFVPGLGFPLSSGGFYHPSYGVPYSSTPLGLGYYRGYTPPSALYPHPHHQAPHSGPSHHVHSPSFPPPPPPSYVHHHHPSHLLLNPSKFHKKKHKLLRQEYLGGGRSSMLYPAMSSELSFGWHHKHKHRHKHRDRCGEEEGDDGGGVLGGIGGRGVGGEGLGAGRGERGSVESLQHCRFGRDGSADSRTNKQSASANSPSSSSTSSAERYKRKEASMSCLGPSRLSLGTASRGHHPVDSWFRMGSSDTDYSKLSRNQSFSGSGAFSEGHVQNPVDCSESDEEDAASPIEEPEISHHTNLFASALSRTSLKGSRSKKGGPLESPSLSRLDRTVRRDRSTSSEKREIGNVSSLQNRVGQSSVPDVSEGLHHRHHHHHHPPLSLSHAASCLPHCTLDQPGNTAPLRPSSRQASPSTCSSQPACCLDSPPSHLSHRPPPKNNLLHVNKILRAKKLQRQARTGNNMVKKRGPGRPRKNPLPSPPATPPPQTSEQDNHPLPERHRATALWEADTVVDAIEAVVQNQRKKGRKRKHWERETEGGDEEEEDAEEERQVEDEEEEEDEEQEQQSAAREDDVMSRVRTEGSRQWLTQEEVHCFRSALEGKPDGSPERPGPATSEPTPNPSVTSQREKRVARPPKKKFQKAGLYSDVYKTDDPRSQLLQLKKEKLEYTPGEHDYGLLPAPIHVGKYLRQKRIDFQLPYDILWLWKHDQLYKRPDVPLYKKIRSNVYVDVKPLSGYEATTCNCRLPDDSSEKGCQDDCLNRMIYAECSPSTCPCSDQCDNQRIQKHEWVQCLERFRAEGKGWGIRTKQPLRAGQFIIEYLGEVVSEQEFRKYIASDCRLHQVKIPMLGENKKQIGVSRMMEQYFSHSGHYCLNLDSGMVIDSYRMGNEARFVNHSCEPNCEMQKWSVNGVYRIGLFALKDINSGTELTYDYNFHSFNTEEQQVCKCGSEGCRGIIGGKSKRINGLPAKGAGSGGGARRLGRLKEKRKSKHSLKKREEESSDSSKFYQHLLMKPMSNRERNFVLKHRVFLLRNWEKMREKQELLKREGERERENSGLSLYTRWGGVIRDDGNIKSDVFLTQFSALQTSRSVRTRRLAAAEENTEVTRTARLAHIFKEICDMITSYKDSSGQPLAAPLLNLPSRKRNTQYYEKVTDPLDLSTIDKQILSGHYKTDEAFDADMLKVFRNAEKYYGRKSAVGRDVCRLRKAYYGARHEAAVQIDEIVGETASEADSSDSLERDHAHHHHHDGGGSHDKDDDVIRCICGMYKDEGLMIQCEKCMVWQHCDCMRLEADVEHYLCEQCDPRPVDREVPMVPQPSYAQSGFIYYICLLRDDLLLHQGDCVYLMRDSRRTTEGQPVRQSYRLLSHINRDKLDIFRIEKLWKNEKGERFAFGHHYFRPHETHHSPSRRFYHNELFRVPLYEIIPLEAVVGTCCVLDLYTYCKGRPKGVKEQDVYICDYRLDKSAHLFYKIHRNRYPVCTKPYAFNHFPKRLTPKRDFSPHYVPDNYKRNGGRSAWKSERPKDEEPSSSSTSCERLSSSFPREGDEDGGRGAEGDAEAAQEGSSTPRPQRSSPLESRTAGEEDEAEEEEDRERRREMQEEEEEEGCREKAGAQEPIDAQCSSSISSSPLHPAILGRREAQRERLNKILLDLLHRAPTKNAIDVTYLLEEGSGRRLRRRTLGLSEFICRK

Target ORF information:

RefSeq Version	XM_005159483.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ash1 (absent, small, or homeotic)-like (Drosophila) (ash1l), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159483.4

ORF Insert Sequence:

ATGGATCAGA GGACACAGGG GGGGCCTTCT ACGCCGCCCC CACTCCCTGC CAATGCCCCC 
CCAGCAGAAC GTGAAAAAGA AGGTGTAGGA GGAAAGAAGG ACGATGAAGA AGAGAAAAAG 
AAACGGGAGA AACTAAGAGA TGGGGCACAG AATGAAGCTA GTGGTGCTGA AAGTGGGACT 
GGTGACCAGC CGCAACAACC CACGCAGTTT TCGGTCAAGG AGACCAGCTA CTCTGAGGGT 
AACGTTAAGC TCAAAATAGG CCTGCAGGCC AAACGCATGA AGAAACCTCC TAAGATCCTG 
GAGAACTATG TCTGCCGACC AGCCTTCCGT GCCACTGTAA GGCATAGTGG AGGTAGAGGC 
GGAGGACGTG CTAATCGTGG GGCTGGAGCC AGTGATGCGA ACAGCAACTC TACTAGCACA 
AGTAATGGAA AGGAAGGGGA GAAAAAGCCC TCTGTCAACC GAGGAGCATC TCAGTCAGCT 
GCTGCTCCTG CTGCTTCTAC TCCCTCACCC TCTCTTCCTC CTGCTTCCAT CAACACTGTC 
ACTCCAGTGA ATGGAAATGC TCCAGCAAAA AGGGGTCCTC CAAAACTTGC CTGCAAATCA 
GAGGGAAAGG CTGACGTAAA ATCTGTTGCT TCATCAGAGA AACCTCTTAA TCTCCATCGC 
CCCACGACAG AAAGTAAATC ACACTCCTCT GGAAAGAAAA ACCATGTTCC ACAAACAAAC 
TCTGCCCCTG CCCCATCTCC ACCAGCAGTG CCCCCAGCTG CCACTTCGCA ACAGGACCCT 
AAAATAGATG GAATCTTAAA AACGCCTCAA TGCAATTTTT TGGAGGGGCA GAAGGAACAA 
GAAAGAGGGA TTTCTTCTTG GGGTACCCCT ACAGTAACTG AAAAACTTGC TCAGCTCATA 
GCTACATGCC CACCTTCAAA GTGCCCTAAG CCAAAAGCCC GAAAGGTATC GCCAGCTCCA 
GCACACAGTA GCTCACCTCC GTTAATTTCT AATCTGCAGC GCCCTGAACG GGCAATGGCT 
AATAGAAACA CCTATTCCAG GGCGGTTCAC CTCGCCCAAC CACCACCTGT ATCACGGCCC 
CCTGGTCGCC CATATGGGTC TAAAAACAAA GACAGTATCC TTGAGAAATT TTCTGGAACT 
TCTAAAAAAG AGGAATCAGA TGGACTTTCA AAAGGGGTCT CCGGTAGCAG CAGCACAACC 
ACCACCACCA CTAGTTCAGC ACTGTCTCAA AACAGCAGTC GCTTGACGAC AACAACATCC 
ACAAATGAAA GCACTGACAT TAACAACACA GATGGAATGA AATCTCAGTC TCGTCTTGGG 
CATTGCCCAT CTCATTCTTC ATCCGCAGCT GCTTCACCTC CATTTTCTTC TTCATCCTCC 
GTAGAGAGAA TAGGAAGTAC AAGCTGTAAC ATAGGTTTGC CAGGCTCTCT AACCTCACGC 
CCTAAAGCAA GCCCCTCTCG AATAGAGGAT TTTAGTGAGA AAGACAAAGA ACGTGACCGG 
GAAAAGGACA CAGACAGTCC AAGAGATTTA AGTAAAGGTA TCACTCCAGC TATAACTGGG 
AAGCAGGATG CAAAAGAAAG GTGTTCTTTA TCAGCTCCTC GAAATGACAG GGGTAAGAGC 
TCAAGCCCAG TCAAACGTAG TCCCAATCAG GAAAGTCGCG TTAGTGGGGT ACCAAGTCCT 
GCTGAGTCCA CCAAATGCAC TATTTCTCCT TCATACCCTT CTGGTGCCAG CAGGAGCCCA 
CCACCACTAT GCGATGACAC CGGGGCTATT TCTCCTGCAT CACCATCTGA GCAGGATTCA 
AAACCTCTTA AGAAGCGACG AGGACGACGA CCTCGCTGGA CGAAGGCAGT GAGTCGCACC 
CACAAGTCTT TACATGAGTC TCCTTCTAAC CATCAGCAAA TCCCCACACC AATGCCCTCA 
GGGCTTTCTT TCTCATCCCC TATTCGGGAG TCTGTGGAGC AGCCTCTCAG TGACAGTTAC 
ACATCCCCAG AGGATTCTGA TATTTTCGAT TCTTCGCCCA AGAAGAGAGG ACGTCCAAAG 
ACCAAAATGC CTCGATTAGA TGCACCAGCA CATGGCTGCA CCCCAAACAA GTTGGCACCT 
TCAAAATCCT TCTCCAGTCT GCTCAAGTCT AAGGAAGAGC AGGACCCCCC TGTTTTGCAC 
CCTGAAGTGG ATTTTAATCC ACCTAAAATG ATGGCAAGAA AGCGAGGGCG ACCCAAGCGC 
TCACCTCCAA CTCTTCCTCA AGAAACCCAG CCTCCGACTC TTGCTCCTGA GTCAGGGGAA 
GCATCAGTTG GGGAAAAACC TTTTCGTAAT AAGGGTAATG GGCAGCTCAT TATGAAGACG 
ATCATAGGCA AAATCAATAA AATGAAGACA GTAAAACGGA AGAGGCTCCT CAGCCAAATA 
CTGCTTGGGC CAAAACCAGC AGAGGAGCAG GAATGCTCAA GAAGCAGTGG GCCAACAGGG 
TCTCCGGTTG CTACAAAACC ACAGTCCCTG ACATCACTTG CTGCTACATT TGGTGGCAAA 
TTAGGGCCCC AGATTAATGT AAGCAAAAAG GGGACAATAT ATATGGGTAA AAGAAGGGGT 
CGTAAGCCAA AAACATCAAC TAATCCTTCC CCAGGTGCCC CCACACCCCC TCCTGAGCCT 
TTCTTATCAC CAAACACTTC ATCCCCTCAT CATCACCAGG TGCAGGCTTT ACAAAACCAA 
CCACACCAGT TGCCTCCATC TGAGGTGTTT CCTTCCCCAT CACTATCACA GTCAAGTGGG 
GGCCAAAGCC CCATTAGTGA TGCTAGCTTT GTAGAGCCTG GCTCAGTGCA TTTCTCATCT 
CACCCACACT GCAGTCATTC TCATCAGGTC CACCACTCAT TCTCATTTCC ACCGCCTACT 
TTTTCAGCCC CCTCTGCTCG ATCTGTAGCA AGTGCTGCAA CATCCACTGC ACACCCATCT 
CAGAAGAAGT CCTCTTGCCG GGGTTACCAT CATCACCACC ACCATTACCG GCAGCACTAT 
CATTATCGAA AATTTTCTCC TCCAAGGCCA CTATTGCCAA CGTCTCCAGC TCCACTGAGT 
GAGCTGAAAG AAGCCACACC TTCTCCAGTG AGTGAATCAC ACAGTGAAGA GACTGTACCC 
AGTGACAGTG GCATTGGAAC AGACAATAAT AGCACCTCTG ACCGTGGGGA AAAAGCTGGA 
AGTGCCAATG GCTTACCTGG TGTGGGGGTC TCACAGGGTA TGGCAGGTGG ACTCTTAATG 
CCTGGAGTCA TAGGTCCAGG TATTGGAACG GGAATTGGAC TTTCTTCGAG AGGTCGGAGG 
AGGCACTCAT CTTCAGTTCT TCTAGAACGT CCCTCACCTA CACCCTCACC TCTTGGAGTG 
AGAGCATCAC CTGACCCAAG AAGATCGCAC CCTGCTGCCC CATCTTCTGC TTTGGTTGGA 
CATAAAGAGA AACACAAGCA TAAGTGCAAG CGTCGTGGGC ATGGCTGCCC TAGCTATGAC 
AAACTAAAAA GGCAAAAGCG TAAACGCAAG AAGAAGTACC TGCAGCTACG GTCCCGCAGA 
CAAGACCCAG ATTTTTTGGC TGATTTAGAT GAGATATGTG TGCAGCTTAG TGATATACGA 
ATTTCTCATC GAACACCTAC ACTTCGTTTG GGCAGCAGCA TAGGTTTGGG TGCCGGGGCT 
GGTCCTGGCC GTGGGTCTGG CTTGGGAAGT ACCAGACCTA GCAGCAGCCT TGGAGGCGGA 
ACCAATCCAC CACCCCATCA CTATCTGCAT AGAGATCTAT TACCCACAAT ATTCAGGATA 
AATTTTAGTG GGTATTATTC ACCCCACCCA GCCTACCCAT GTGATTCACT TCATTACGTT 
CGTAAGCCAG ATCTTAAAAA GAAAAGAGGT CGACCACCTA AGCTTCGGGA ATCCATATCA 
GAAGTACCTT TTGTACCTGG TTTAGGCTTT CCCCTCTCAA GTGGAGGTTT CTATCACCCT 
TCTTATGGTG TCCCATACTC TTCTACCCCA CTGGGACTTG GTTACTATCG TGGTTATACC 
CCACCAAGTG CCCTGTACCC CCATCCTCAT CACCAGGCTC CCCACTCTGG CCCATCCCAC 
CATGTTCATT CACCATCTTT TCCTCCACCT CCACCCCCTT CTTATGTGCA TCATCATCAC 
CCATCCCACC TGCTTCTTAA CCCCTCCAAA TTTCACAAGA AGAAACATAA GCTACTTAGG 
CAAGAGTATT TAGGTGGTGG TCGTTCATCC ATGCTCTATC CTGCCATGTC ATCTGAGCTG 
TCATTTGGCT GGCACCACAA ACACAAGCAT AGGCACAAAC ACCGTGATCG CTGTGGAGAA 
GAGGAGGGTG ATGATGGTGG TGGAGTTTTA GGTGGTATAG GAGGCAGAGG TGTTGGAGGT 
GAGGGCTTGG GGGCCGGAAG AGGGGAGAGA GGGAGCGTAG AGTCCCTGCA GCACTGCCGG 
TTTGGACGAG ATGGCTCTGC TGACTCCCGA ACTAACAAAC AGTCAGCTTC TGCCAACTCC 
CCTTCCTCTT CCTCCACATC CTCTGCAGAA CGGTACAAGC GAAAAGAGGC CTCTATGTCT 
TGTCTTGGGC CCTCACGACT GTCTTTGGGC ACTGCAAGTC GGGGACATCA TCCTGTTGAC 
TCCTGGTTCA GAATGGGAAG TTCTGATACT GATTACTCCA AGCTCTCTAG AAACCAGTCT 
TTTTCGGGGT CTGGTGCATT CTCTGAAGGA CATGTGCAAA ATCCAGTGGA TTGCTCAGAG 
AGTGATGAGG AAGATGCTGC TTCCCCCATT GAAGAGCCAG AGATCAGCCA TCACACAAAC 
CTGTTTGCAT CTGCACTATC AAGGACCTCC CTCAAAGGAA GTCGCAGCAA AAAAGGTGGA 
CCATTAGAGA GTCCCAGTCT CTCACGGTTG GACAGGACGG TTAGAAGGGA TCGTTCAACA 
TCTTCAGAGA AAAGAGAAAT TGGGAATGTA TCTAGCTTGC AGAACAGAGT TGGCCAATCC 
AGTGTTCCTG ATGTTTCTGA GGGTCTCCAT CATCGGCACC ACCATCACCA CCACCCTCCT 
CTATCCCTCT CGCACGCTGC CTCTTGCCTG CCGCACTGCA CTCTAGACCA GCCTGGAAAC 
ACTGCACCAC TCCGGCCCTC CAGCCGCCAA GCTAGTCCAT CCACATGCTC CTCCCAGCCT 
GCATGCTGCC TTGATTCCCC ACCTTCACAT CTCTCCCACC GGCCCCCTCC CAAAAACAAC 
CTGCTTCATG TCAACAAGAT CCTCCGTGCA AAGAAGCTCC AAAGGCAAGC ACGTACAGGG 
AACAACATGG TGAAAAAACG GGGTCCAGGA CGTCCCCGCA AGAACCCGCT GCCCTCACCT 
CCTGCAACAC CCCCACCACA GACTTCCGAA CAAGACAACC ACCCATTACC TGAAAGGCAC 
CGAGCAACTG CACTCTGGGA GGCAGATACT GTAGTTGATG CCATTGAGGC TGTCGTACAG 
AACCAGCGCA AAAAGGGACG CAAGCGGAAG CACTGGGAAA GGGAGACTGA AGGAGGTGAT 
GAAGAAGAGG AGGATGCGGA GGAAGAAAGA CAGGTGGAGG ACGAAGAAGA AGAAGAAGAC 
GAAGAACAAG AACAACAGTC AGCTGCTAGA GAAGACGATG TAATGTCGAG AGTTAGAACA 
GAAGGAAGCA GGCAATGGTT GACCCAGGAA GAAGTGCACT GTTTTCGCAG TGCACTGGAG 
GGAAAACCTG ATGGCTCCCC TGAGCGCCCA GGCCCTGCTA CTTCGGAACC AACCCCAAAC 
CCATCAGTGA CCAGCCAACG TGAGAAGAGA GTTGCTAGAC CACCCAAAAA GAAGTTTCAA 
AAGGCGGGAC TTTACTCTGA TGTGTACAAG ACTGATGATC CTCGCAGTCA GCTGCTACAG 
TTAAAGAAAG AGAAGCTAGA ATACACTCCA GGTGAACATG ACTATGGCCT TCTTCCTGCT 
CCCATTCATG TTGGGAAATA CTTGAGACAG AAACGCATTG ATTTCCAGCT GCCTTACGAC 
ATCCTGTGGC TTTGGAAACA TGATCAGCTC TACAAGAGAC CTGACGTGCC TCTCTACAAG 
AAGATCAGAT CCAATGTCTA TGTGGATGTG AAGCCTCTTT CTGGCTATGA AGCCACTACC 
TGTAACTGCA GGTTGCCTGA CGACTCTAGT GAAAAGGGCT GTCAAGATGA TTGTCTAAAC 
AGGATGATTT ATGCCGAGTG CTCTCCAAGT ACGTGTCCGT GTTCCGATCA GTGCGATAAC 
CAGCGGATCC AGAAGCATGA GTGGGTTCAG TGCCTCGAGC GCTTCCGAGC TGAGGGAAAG 
GGCTGGGGCA TCCGGACCAA GCAGCCCCTT CGTGCCGGCC AATTTATCAT CGAGTACTTG 
GGAGAGGTGG TCAGCGAACA AGAGTTCAGA AAATACATTG CGTCTGACTG TAGGTTGCAC 
CAAGTCAAAA TTCCAATGTT GGGGGAAAAT AAAAAGCAGA TTGGTGTGAG TCGCATGATG 
GAGCAGTACT TCTCTCACAG CGGCCACTAC TGCCTGAATC TGGACAGCGG CATGGTCATC 
GATAGCTACC GCATGGGCAA CGAGGCTCGC TTTGTCAACC ACAGCTGCGA GCCCAACTGT 
GAGATGCAGA AATGGTCGGT GAACGGAGTG TACCGAATTG GCCTGTTTGC TCTAAAAGAC 
ATCAACAGCG GAACGGAACT GACATATGAC TACAACTTCC ACTCCTTCAA CACAGAGGAA 
CAACAAGTCT GCAAGTGTGG CTCAGAGGGT TGCCGAGGCA TCATTGGCGG CAAGAGCAAG 
CGAATCAATG GGCTGCCTGC TAAAGGGGCC GGATCTGGAG GCGGAGCCCG GAGACTTGGA 
AGATTGAAAG AGAAACGCAA GTCCAAACAC TCACTGAAGA AGCGGGAGGA AGAGTCCAGT 
GACAGCAGCA AGTTTTACCA GCACCTTTTA ATGAAGCCCA TGTCAAACAG AGAGAGGAAC 
TTTGTGCTGA AACATCGGGT CTTTCTGCTG CGGAATTGGG AGAAGATGCG GGAGAAACAG 
GAGCTTCTGA AGCGTGAGGG AGAGCGGGAG AGGGAGAACA GCGGTCTGTC TCTGTACACC 
CGCTGGGGAG GAGTCATCCG GGATGATGGA AACATCAAAT CAGATGTATT TCTGACTCAG 
TTCTCAGCGC TGCAGACGTC CCGCTCTGTA CGCACACGCA GACTTGCGGC AGCCGAGGAG 
AACACTGAAG TGACACGCAC CGCACGTCTG GCACACATTT TCAAAGAGAT CTGCGATATG 
ATCACTAGCT ACAAGGATTC ATCTGGTCAG CCTCTTGCTG CTCCGCTGCT TAACCTGCCT 
TCTAGGAAGA GGAACACACA ATACTATGAG AAGGTCACCG ATCCTCTAGA TCTCAGCACC 
ATAGATAAGC AGATCCTCAG CGGACACTAC AAAACCGATG AGGCGTTTGA TGCGGACATG 
CTCAAAGTGT TTCGCAATGC TGAGAAGTAC TATGGGCGTA AGTCTGCTGT GGGCCGTGAC 
GTGTGCCGGC TGAGGAAGGC TTATTATGGT GCCCGTCATG AGGCTGCTGT TCAGATCGAT 
GAGATTGTGG GTGAGACGGC AAGTGAAGCG GACAGCTCTG ATTCGCTGGA GAGAGACCAC 
GCCCACCACC ATCACCATGA CGGAGGGGGC AGCCATGATA AGGATGATGA TGTGATCCGC 
TGCATCTGTG GCATGTACAA GGATGAGGGA CTCATGATTC AGTGCGAGAA GTGCATGGTG 
TGGCAGCACT GTGATTGTAT GCGTCTGGAG GCGGATGTGG AGCATTATCT CTGTGAACAG 
TGTGACCCTC GTCCAGTGGA CAGAGAGGTT CCAATGGTGC CTCAACCCAG CTATGCCCAA 
TCAGGATTCA TCTATTACAT ATGCCTGCTT CGCGATGACT TACTGTTGCA TCAGGGCGAT 
TGTGTGTACC TCATGCGGGA CAGCAGACGC ACAACAGAAG GTCAGCCGGT GCGTCAGTCA 
TACCGCCTCC TGTCTCACAT CAACAGAGAC AAACTGGACA TCTTCCGTAT CGAGAAACTC 
TGGAAGAATG AAAAAGGTGA GAGGTTTGCA TTTGGTCATC ATTACTTCCG TCCTCATGAG 
ACGCATCACT CCCCTTCCCG CCGCTTCTAC CACAACGAGC TGTTTCGAGT GCCTCTGTAT 
GAGATCATCC CGCTGGAGGC CGTGGTGGGC ACCTGCTGCG TGCTGGACCT CTACACATAC 
TGCAAGGGTC GTCCAAAAGG GGTAAAGGAG CAGGACGTTT ACATCTGCGA CTACCGGCTG 
GATAAATCCG CACACCTGTT CTACAAGATC CACCGCAACC GTTACCCAGT GTGCACCAAG 
CCCTATGCGT TCAACCACTT CCCCAAGAGA CTGACGCCCA AGAGGGACTT CTCTCCTCAC 
TATGTTCCAG ATAACTACAA GAGGAACGGC GGACGCTCTG CTTGGAAGAG CGAGCGGCCC 
AAAGATGAAG AACCATCATC CTCCAGCACG TCCTGCGAGC GTCTGTCCTC CAGCTTCCCT 
AGAGAAGGAG ACGAGGACGG AGGACGAGGA GCAGAAGGAG ACGCGGAGGC CGCTCAAGAG 
GGATCCAGCA CTCCGAGACC TCAGAGATCC TCGCCACTGG AGAGCAGGAC GGCCGGAGAG 
GAGGATGAGG CGGAGGAGGA AGAGGACAGA GAGAGGAGGA GAGAGATGCA GGAGGAGGAG 
GAGGAGGAGG GATGCAGAGA GAAAGCAGGA GCGCAGGAGC CCATAGACGC ACAGTGCTCC 
TCCTCCATCT CCTCTTCTCC TCTTCATCCC GCCATTCTGG GCAGGAGAGA AGCGCAGAGA 
GAGCGGCTCA ACAAGATCCT GCTCGACCTG CTGCACCGCG CGCCCACTAA GAACGCGATA 
GATGTGACGT ATCTTCTTGA GGAAGGCTCG GGTCGTCGCC TTCGCAGACG GACGCTTGGA 
CTGAGCGAAT TCATTTGCAG AAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Nature cell biology15(12)1516-25(2013 Dec)
Huang HT,Kathrein KL,Barton A,Gitlin Z,Huang YH,Ward TP,Hofmann O,Dibiase A,Song A,Tyekucheva S,Hide W,Zhou Y,Zon LI

Genome-wide survey and developmental expression mapping of zebrafish SET domain-containing genes.
PloS one3(1)e1499(2008 Jan)
Sun XJ,Xu PF,Zhou T,Hu M,Fu CT,Zhang Y,Jin Y,Chen Y,Chen SJ,Huang QH,Liu TX,Chen Z

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ash1l cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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