adgrb1a cDNA ORF clone, Danio rerio(zebrafish)

The following adgrb1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the adgrb1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa149432 XM_021466983.1
Latest version!
Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa44103 XM_009292561.2
Latest version!
Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa149429 XM_021466980.1
Latest version!
Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa149430 XM_021466981.1
Latest version!
Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa149431 XM_021466982.1
Latest version!
Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa44100 XM_021466979.1
Latest version!
Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa44094 XM_009292551.3
Latest version!
Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa44095 XM_009292552.3
Latest version!
Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa44096 XM_009292553.3
Latest version!
Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa44097 XM_009292554.3
Latest version!
Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa44098 XM_009292555.3
Latest version!
Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa44099 XM_009292556.3
Latest version!
Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa44101 XM_009292559.3
Latest version!
Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa44102 XM_009292560.3
Latest version!
Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa44104 XM_009292562.3
Latest version!
Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
View Old Accession Versions >>
ODa44097 XM_009292554.2 Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa44094 XM_009292551.2 Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa44104 XM_009292562.2 Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa44102 XM_009292560.2 Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa44099 XM_009292556.2 Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa44095 XM_009292552.2 Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa44098 XM_009292555.2 Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa44096 XM_009292553.2 Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa44101 XM_009292559.2 Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa149432
    Clone ID Related Accession (Same CDS sequence) XM_021466983.1
    Accession Version XM_021466983.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3786bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product brain-specific angiogenesis inhibitor 1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCT ACAGGCAGTA TTCCTCAAAA
    GGCCAGGGTT TAAGTCCAGT GAAATACAAA GGAATGCAAG AGGCCATGCT TGGCAAAGGC
    CGGCTTGGCT CTCGTAGCCA ATCCCTGCGC TCGGTGGACA GCCGTAAACG AGAGGACTCT
    GATAAGACCC GCAGCGGACA GCAGTCTTCC CAAACAGTGG ATTCTCCGTC GGGTGAGGAG
    TGGTCTGCGT GGAGCGTGTG TTCGGCCACG TGTGGTGAGG GCTGGCAGAG CCGCACACGC
    TTCTGCGTCT CCGTGTCGTA CAGCACGCAG TGCAGCGGGC CACTGCGCGA ACAGAGACCC
    TGCAATAACA CTGCTGTGTG TCCAGCCGCT CTGATTACAG AACATGGGGC GTGGGGCGAG
    TGGACACCAT GGAGCTTGTG TTCATCAACA TGCGGCCGTG GCTACAGGGA ACGCAACCGC
    TCCTGCAAGC CACCCCAGTT CGGAGGAAAC CCCTGTGATG GTCCAGAGAA ACAGACTAAA
    TTCTGCAACA TCGCTGTATG CCCAGTGGAT GGCGTGTGGA ACGAGTGGTC TGGTTGGAGT
    TCCTGTTCTT CAACTTGTTC GAATGGAACA ATGCAGCGAA TTCGGGAGTG TAACGGGCCC
    TCGTATGGGG GCTTAGAGTG TCAAGGCGAG TGGCACGAGA CACGGGATTG CTTCCTTAGA
    GAATGTCCTG TGGATGGCCA ATGGCAACTT TGGAGCACTT GGTCTGGTTG CACCAAAACC
    TGTGGTGGAG GAAGTCAGCA GAGACAGAGG CTGTGTTATG GTCCCTTTTT TGGGGGAGAG
    GCATGCCCGG GGGACCGAGA AGAAGTTAGA CGTTGCAATG AGAAGCGATG CCCAGAGCCA
    CATGAAATCT GTGATGAGGA GAATTTCTCT AATGTAGTGT GGAAGAAAAC ACCTGCAGGG
    GACACAGCGG CTGTCCGCTG CCCACCAAAT GCTGTAGGAT TAATTCTACG GCGATGTTCA
    TTAGACAATG AGGGTATAGC ATACTGGGAG AACCCAACAT ATATGAAGTG CGTCTCAAAT
    GATTACAGAA GTATCCAAAC ACTGACCAGA GATCACCTGT CCAAGGCGCA GCGAGGGCTT
    GTGGGGGATG GTGTTTCTGA AGTGATGACA AAGCTGAGGG TGACGTCAAG TGATGGAACG
    AGCTACAGCG GAGATCTTTT GGCTATCATA GATGTGCTAA AAAACATGAC AGAGATATTT
    AGGAGATCCT ACTACAGCCC CAGTAATGCA GACATGAGGA ATTTTGTGCA GTCTGTGAGT
    AACTTACTGA TGGAGGAAAA CAGGGACAGG TGGGAGGAAG CACAACTGCT GGGACCCAAT
    GTTAAGGAGC TTTTTCGTCT AGTAGAGGAC TTTGTGGACG TCATCAGCCT TCGCATGAAG
    GACTTCCAGG ATACTTATGA GGTCACGGAT AATCTAGTGT TGAGCATTCA CAAGCGGCCC
    GTGGCTGGAA ATGCAGATAT CACTTTCCCT ATGAAGGGCT GGAGGGGGAT GGTGGACTGG
    GCTCGCAGCT CTGAGGACAA AGTCAGAGTG GCCAAAAACA TCCTGCTCAC TGGAAAACCG
    GAAGAAGATG ACGCTTCAGC TTTTGTGACT GGGATCGTCC TGTACAGAAA CTTAGGCAGT
    ATTCTCTCTC TGCAGAGAAA TAGCACAGTG CTGAACTCCA AGGTGATCTC TGTGACAATC
    AAGCCCACTC CCAGCTCTCT CTCGAGTCCA CTAGAGATTG AGTTCTCACA CCTTTACAAT
    GGAACCACAA ACCAAACCTG CATTTCCTGG GATGAGAACG ACAGCTCGTC GTTGCTGGGC
    TCCTGGTCTG CGCGAGGCTG TAGAGCCGTG CCCGTCGACT CCAGCACAAC CAAATGCTTG
    TGTGACAGAC TCTCCACCTT CGCCATTTTA GCACGCCTCA ATCCCGATGT GAACATGGAT
    AAGACCCAGC TTCCATCTGT GACTCTGATC GTGGGCTGCG GGGTCTCGTC CCTCACCCTG
    CTCCTCCTCA TCATCATCTA TGTTTCTGTT TGGAGGTACA TCCGCTCAGA GCGCTCTGTC
    ATCCTCATCA ACTTCTGTCT GTCCATCATC TCCTCCAATG CGCTTATACT CATCGGTCAA
    ACTCAGACAC GGAACAAGGT TGTTTGTACT CTCGTGGCTG CGTTTCTGCA CTTTTTCTTC
    CTGTCCTCTT TCTGTTGGGT TCTAACGGAG GCATGGCAGT CGTACATGGC TGTGACCGGC
    CGCCTTAGAA ACCGTATCAT ACGCAAGCGT TTCCTTTGCC TTGGTTGGGG TTTACCTGCT
    CTCGTTGTAG CAATTTCTGT TGGATTCACC AAGGCAAAGG GATATGGCAC TGTTAACTAT
    TGCTGGCTGT CTCTGGAAGG AGGGCTGTTG TACGCATTTG TGGGACCAGC TGCTGCTGTC
    GTCCTGGTTA ACATGGTTAT CGGGATTCTG GTTTTTAACA AACTGGTGTC CAAAGATGGC
    ATTACTGATA TGAAACTCAA GGAGAGGGCC GGTCAGATGA CAGTGCCACT GTACAATATG
    ACGCTCAAAT GTGCCAAGTG CGGAGTTATC TCGTCGCCTG ACGTTTCCAC CACAGCTACC
    AGTAACGCCA TGGCGTCGCT GTGGAGCTCC TGCGTTGTTC TGCCGTTGCT GGCGCTCACT
    TGGATGTCGG CAGTCTTGGC TATTACTGAC CGCCGTTCAG CCTTGTTTCA AATCCTTTTT
    GCCGTCTTTG ACTCCCTCGA GGGCTTTGTT ATCGTGATGG TGCATTGCAT CCTACGCAGA
    GAGGTTCAAG AAGCAGTAAA GTGTCGTGTT GTTGACCGTC AAGAAGATGG AAACGGTGAC
    TCTGGTGGAT CCTTCCAGAA CGGTCATGCT CAGCTGATGT TTTTGTTCTT TTTTTCTACA
    GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322658.1
    CDS752..4537
    Translation

    Target ORF information:

    RefSeq Version XM_021466983.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466983.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCT ACAGGCAGTA TTCCTCAAAA
    GGCCAGGGTT TAAGTCCAGT GAAATACAAA GGAATGCAAG AGGCCATGCT TGGCAAAGGC
    CGGCTTGGCT CTCGTAGCCA ATCCCTGCGC TCGGTGGACA GCCGTAAACG AGAGGACTCT
    GATAAGACCC GCAGCGGACA GCAGTCTTCC CAAACAGTGG ATTCTCCGTC GGGTGAGGAG
    TGGTCTGCGT GGAGCGTGTG TTCGGCCACG TGTGGTGAGG GCTGGCAGAG CCGCACACGC
    TTCTGCGTCT CCGTGTCGTA CAGCACGCAG TGCAGCGGGC CACTGCGCGA ACAGAGACCC
    TGCAATAACA CTGCTGTGTG TCCAGCCGCT CTGATTACAG AACATGGGGC GTGGGGCGAG
    TGGACACCAT GGAGCTTGTG TTCATCAACA TGCGGCCGTG GCTACAGGGA ACGCAACCGC
    TCCTGCAAGC CACCCCAGTT CGGAGGAAAC CCCTGTGATG GTCCAGAGAA ACAGACTAAA
    TTCTGCAACA TCGCTGTATG CCCAGTGGAT GGCGTGTGGA ACGAGTGGTC TGGTTGGAGT
    TCCTGTTCTT CAACTTGTTC GAATGGAACA ATGCAGCGAA TTCGGGAGTG TAACGGGCCC
    TCGTATGGGG GCTTAGAGTG TCAAGGCGAG TGGCACGAGA CACGGGATTG CTTCCTTAGA
    GAATGTCCTG TGGATGGCCA ATGGCAACTT TGGAGCACTT GGTCTGGTTG CACCAAAACC
    TGTGGTGGAG GAAGTCAGCA GAGACAGAGG CTGTGTTATG GTCCCTTTTT TGGGGGAGAG
    GCATGCCCGG GGGACCGAGA AGAAGTTAGA CGTTGCAATG AGAAGCGATG CCCAGAGCCA
    CATGAAATCT GTGATGAGGA GAATTTCTCT AATGTAGTGT GGAAGAAAAC ACCTGCAGGG
    GACACAGCGG CTGTCCGCTG CCCACCAAAT GCTGTAGGAT TAATTCTACG GCGATGTTCA
    TTAGACAATG AGGGTATAGC ATACTGGGAG AACCCAACAT ATATGAAGTG CGTCTCAAAT
    GATTACAGAA GTATCCAAAC ACTGACCAGA GATCACCTGT CCAAGGCGCA GCGAGGGCTT
    GTGGGGGATG GTGTTTCTGA AGTGATGACA AAGCTGAGGG TGACGTCAAG TGATGGAACG
    AGCTACAGCG GAGATCTTTT GGCTATCATA GATGTGCTAA AAAACATGAC AGAGATATTT
    AGGAGATCCT ACTACAGCCC CAGTAATGCA GACATGAGGA ATTTTGTGCA GTCTGTGAGT
    AACTTACTGA TGGAGGAAAA CAGGGACAGG TGGGAGGAAG CACAACTGCT GGGACCCAAT
    GTTAAGGAGC TTTTTCGTCT AGTAGAGGAC TTTGTGGACG TCATCAGCCT TCGCATGAAG
    GACTTCCAGG ATACTTATGA GGTCACGGAT AATCTAGTGT TGAGCATTCA CAAGCGGCCC
    GTGGCTGGAA ATGCAGATAT CACTTTCCCT ATGAAGGGCT GGAGGGGGAT GGTGGACTGG
    GCTCGCAGCT CTGAGGACAA AGTCAGAGTG GCCAAAAACA TCCTGCTCAC TGGAAAACCG
    GAAGAAGATG ACGCTTCAGC TTTTGTGACT GGGATCGTCC TGTACAGAAA CTTAGGCAGT
    ATTCTCTCTC TGCAGAGAAA TAGCACAGTG CTGAACTCCA AGGTGATCTC TGTGACAATC
    AAGCCCACTC CCAGCTCTCT CTCGAGTCCA CTAGAGATTG AGTTCTCACA CCTTTACAAT
    GGAACCACAA ACCAAACCTG CATTTCCTGG GATGAGAACG ACAGCTCGTC GTTGCTGGGC
    TCCTGGTCTG CGCGAGGCTG TAGAGCCGTG CCCGTCGACT CCAGCACAAC CAAATGCTTG
    TGTGACAGAC TCTCCACCTT CGCCATTTTA GCACGCCTCA ATCCCGATGT GAACATGGAT
    AAGACCCAGC TTCCATCTGT GACTCTGATC GTGGGCTGCG GGGTCTCGTC CCTCACCCTG
    CTCCTCCTCA TCATCATCTA TGTTTCTGTT TGGAGGTACA TCCGCTCAGA GCGCTCTGTC
    ATCCTCATCA ACTTCTGTCT GTCCATCATC TCCTCCAATG CGCTTATACT CATCGGTCAA
    ACTCAGACAC GGAACAAGGT TGTTTGTACT CTCGTGGCTG CGTTTCTGCA CTTTTTCTTC
    CTGTCCTCTT TCTGTTGGGT TCTAACGGAG GCATGGCAGT CGTACATGGC TGTGACCGGC
    CGCCTTAGAA ACCGTATCAT ACGCAAGCGT TTCCTTTGCC TTGGTTGGGG TTTACCTGCT
    CTCGTTGTAG CAATTTCTGT TGGATTCACC AAGGCAAAGG GATATGGCAC TGTTAACTAT
    TGCTGGCTGT CTCTGGAAGG AGGGCTGTTG TACGCATTTG TGGGACCAGC TGCTGCTGTC
    GTCCTGGTTA ACATGGTTAT CGGGATTCTG GTTTTTAACA AACTGGTGTC CAAAGATGGC
    ATTACTGATA TGAAACTCAA GGAGAGGGCC GGTCAGATGA CAGTGCCACT GTACAATATG
    ACGCTCAAAT GTGCCAAGTG CGGAGTTATC TCGTCGCCTG ACGTTTCCAC CACAGCTACC
    AGTAACGCCA TGGCGTCGCT GTGGAGCTCC TGCGTTGTTC TGCCGTTGCT GGCGCTCACT
    TGGATGTCGG CAGTCTTGGC TATTACTGAC CGCCGTTCAG CCTTGTTTCA AATCCTTTTT
    GCCGTCTTTG ACTCCCTCGA GGGCTTTGTT ATCGTGATGG TGCATTGCAT CCTACGCAGA
    GAGGTTCAAG AAGCAGTAAA GTGTCGTGTT GTTGACCGTC AAGAAGATGG AAACGGTGAC
    TCTGGTGGAT CCTTCCAGAA CGGTCATGCT CAGCTGATGT TTTTGTTCTT TTTTTCTACA
    GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44103
    Clone ID Related Accession (Same CDS sequence) XM_009292561.2
    Accession Version XM_009292561.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3039bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product brain-specific angiogenesis inhibitor 1 isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292561.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCT ACAGGCAGTA TTCCTCAAAA
    GGCCAGGGTT TAAGTCCAGT GAAATACAAA GGAATGCAAG AGGCCATGCT TGGCAAAGGC
    CGGCTTGGCT CTCGTAGCCA ATCCCTGCGC TCGGTGGACA GCCGTAAACG AGAGGACTCT
    GATAAGACCC GCAGCGGACA GCAGTCTTCC CAAACAGTGG ATTCTCCGTC GGGTGAGGAG
    TGGTCTGCGT GGAGCGTGTG TTCGGCCACG TGTGGTGAGG GCTGGCAGAG CCGCACACGC
    TTCTGCGTCT CCGTGTCGTA CAGCACGCAG TGCAGCGGGC CACTGCGCGA ACAGAGACCC
    TGCAATAACA CTGCTGTGTG TCCAGCCGCT CTGATTACAG AACATGGGGC GTGGGGCGAG
    TGGACACCAT GGAGCTTGTG TTCATCAACA TGCGGCCGTG GCTACAGGGA ACGCAACCGC
    TCCTGCAAGC CACCCCAGTT CGGAGGAAAC CCCTGTGATG GTCCAGAGAA ACAGACTAAA
    TTCTGCAACA TCGCTGTATG CCCAGTGGAT GGCGTGTGGA ACGAGTGGTC TGGTTGGAGT
    TCCTGTTCTT CAACTTGTTC GAATGGAACA ATGCAGCGAA TTCGGGAGTG TAACGGGCCC
    TCGTATGGGG GCTTAGAGTG TCAAGGCGAG TGGCACGAGA CACGGGATTG CTTCCTTAGA
    GAATGTCCTG TGGATGGCCA ATGGCAACTT TGGAGCACTT GGTCTGGTTG CACCAAAACC
    TGTGGTGGAG GAAGTCAGCA GAGACAGAGG CTGTGTTATG GTCCCTTTTT TGGGGGAGAG
    GCATGCCCGG GGGACCGAGA AGAAGTTAGA CGTTGCAATG AGAAGCGATG CCCAGAGCCA
    CATGAAATCT GTGATGAGGA GAATTTCTCT AATGTAGTGT GGAAGAAAAC ACCTGCAGGG
    GACACAGCGG CTGTCCGCTG CCCACCAAAT GCTGTAGGAT TAATTCTACG GCGATGTTCA
    TTAGACAATG AGGGTATAGC ATACTGGGAG AACCCAACAT ATATGAAGTG CGTCTCAAAT
    GATTACAGAA GTATCCAAAC ACTGACCAGA GATCACCTGT CCAAGGCGCA GCGAGGGCTT
    GTGGGGGATG GTGTTTCTGA AGTGATGACA AAGCTGAGGG TGACGTCAAG TGATGGAACG
    AGCTACAGCG GAGATCTTTT GGCTATCATA GATGTGCTAA AAAACATGAC AGAGATATTT
    AGGAGATCCT ACTACAGCCC CAGTAATGCA GACATGAGGA ATTTTGTGCA GTCTGTGAGT
    AACTTACTGA TGGAGGAAAA CAGGGACAGG TGGGAGGAAG CACAACTGCT GGGACCCAAT
    GTTAAGGAGC TTTTTCGTCT AGTAGAGGAC TTTGTGGACG TCATCAGCCT TCGCATGAAG
    GACTTCCAGG ATACTTATGA GGTCACGGAT AATCTAGTGT TGAGCATTCA CAAGCGGCCC
    GTGGCTGGAA ATGCAGATAT CACTTTCCCT ATGAAGGGCT GGAGGGGGAT GGTGGACTGG
    GCTCGCAGCT CTGAGGACAA AGTCAGAGTG GCCAAAAACA TCCTGCTCAC TGGAAAACCG
    GAAGAAGATG ACGCTTCAGC TTTTGTGACT GGGATCGTCC TGTACAGAAA CTTAGGCAGT
    ATTCTCTCTC TGCAGAGAAA TAGCACAGTG CTGAACTCCA AGGTGATCTC TGTGACAATC
    AAGCCCACTC CCAGCTCTCT CTCGAGTCCA CTAGAGATTG AGTTCTCACA CCTTTACAAT
    GGAACCACAA ACCAAACCTG CATTTCCTGG GATGAGAACG ACAGCTCGTC GTTGCTGGGC
    TCCTGGTCTG CGCGAGGCTG TAGAGCCGTG CCCGTCGACT CCAGCACAAC CAAATGCTTG
    TGTGACAGAC TCTCCACCTT CGCCATTTTA GCACGCCTCA ATCCCGATGT GAACATGGAT
    AAGACCCAGC TTCCATCTGT GACTCTGATC GTGGGCTGCG GGGTCTCGTC CCTCACCCTG
    CTCCTCCTCA TCATCATCTA TGTTTCTGTT TGGAGGTACA TCCGCTCAGA GCGCTCTGTC
    ATCCTCATCA ACTTCTGTCT GTCCATCATC TCCTCCAATG CGCTTATACT CATCGGTCAA
    ACTCAGACAC GGAACAAGGT TTACCTGCTC TCGTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290836.1
    CDS731..3769
    Translation

    Target ORF information:

    RefSeq Version XM_009292561.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292561.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCT ACAGGCAGTA TTCCTCAAAA
    GGCCAGGGTT TAAGTCCAGT GAAATACAAA GGAATGCAAG AGGCCATGCT TGGCAAAGGC
    CGGCTTGGCT CTCGTAGCCA ATCCCTGCGC TCGGTGGACA GCCGTAAACG AGAGGACTCT
    GATAAGACCC GCAGCGGACA GCAGTCTTCC CAAACAGTGG ATTCTCCGTC GGGTGAGGAG
    TGGTCTGCGT GGAGCGTGTG TTCGGCCACG TGTGGTGAGG GCTGGCAGAG CCGCACACGC
    TTCTGCGTCT CCGTGTCGTA CAGCACGCAG TGCAGCGGGC CACTGCGCGA ACAGAGACCC
    TGCAATAACA CTGCTGTGTG TCCAGCCGCT CTGATTACAG AACATGGGGC GTGGGGCGAG
    TGGACACCAT GGAGCTTGTG TTCATCAACA TGCGGCCGTG GCTACAGGGA ACGCAACCGC
    TCCTGCAAGC CACCCCAGTT CGGAGGAAAC CCCTGTGATG GTCCAGAGAA ACAGACTAAA
    TTCTGCAACA TCGCTGTATG CCCAGTGGAT GGCGTGTGGA ACGAGTGGTC TGGTTGGAGT
    TCCTGTTCTT CAACTTGTTC GAATGGAACA ATGCAGCGAA TTCGGGAGTG TAACGGGCCC
    TCGTATGGGG GCTTAGAGTG TCAAGGCGAG TGGCACGAGA CACGGGATTG CTTCCTTAGA
    GAATGTCCTG TGGATGGCCA ATGGCAACTT TGGAGCACTT GGTCTGGTTG CACCAAAACC
    TGTGGTGGAG GAAGTCAGCA GAGACAGAGG CTGTGTTATG GTCCCTTTTT TGGGGGAGAG
    GCATGCCCGG GGGACCGAGA AGAAGTTAGA CGTTGCAATG AGAAGCGATG CCCAGAGCCA
    CATGAAATCT GTGATGAGGA GAATTTCTCT AATGTAGTGT GGAAGAAAAC ACCTGCAGGG
    GACACAGCGG CTGTCCGCTG CCCACCAAAT GCTGTAGGAT TAATTCTACG GCGATGTTCA
    TTAGACAATG AGGGTATAGC ATACTGGGAG AACCCAACAT ATATGAAGTG CGTCTCAAAT
    GATTACAGAA GTATCCAAAC ACTGACCAGA GATCACCTGT CCAAGGCGCA GCGAGGGCTT
    GTGGGGGATG GTGTTTCTGA AGTGATGACA AAGCTGAGGG TGACGTCAAG TGATGGAACG
    AGCTACAGCG GAGATCTTTT GGCTATCATA GATGTGCTAA AAAACATGAC AGAGATATTT
    AGGAGATCCT ACTACAGCCC CAGTAATGCA GACATGAGGA ATTTTGTGCA GTCTGTGAGT
    AACTTACTGA TGGAGGAAAA CAGGGACAGG TGGGAGGAAG CACAACTGCT GGGACCCAAT
    GTTAAGGAGC TTTTTCGTCT AGTAGAGGAC TTTGTGGACG TCATCAGCCT TCGCATGAAG
    GACTTCCAGG ATACTTATGA GGTCACGGAT AATCTAGTGT TGAGCATTCA CAAGCGGCCC
    GTGGCTGGAA ATGCAGATAT CACTTTCCCT ATGAAGGGCT GGAGGGGGAT GGTGGACTGG
    GCTCGCAGCT CTGAGGACAA AGTCAGAGTG GCCAAAAACA TCCTGCTCAC TGGAAAACCG
    GAAGAAGATG ACGCTTCAGC TTTTGTGACT GGGATCGTCC TGTACAGAAA CTTAGGCAGT
    ATTCTCTCTC TGCAGAGAAA TAGCACAGTG CTGAACTCCA AGGTGATCTC TGTGACAATC
    AAGCCCACTC CCAGCTCTCT CTCGAGTCCA CTAGAGATTG AGTTCTCACA CCTTTACAAT
    GGAACCACAA ACCAAACCTG CATTTCCTGG GATGAGAACG ACAGCTCGTC GTTGCTGGGC
    TCCTGGTCTG CGCGAGGCTG TAGAGCCGTG CCCGTCGACT CCAGCACAAC CAAATGCTTG
    TGTGACAGAC TCTCCACCTT CGCCATTTTA GCACGCCTCA ATCCCGATGT GAACATGGAT
    AAGACCCAGC TTCCATCTGT GACTCTGATC GTGGGCTGCG GGGTCTCGTC CCTCACCCTG
    CTCCTCCTCA TCATCATCTA TGTTTCTGTT TGGAGGTACA TCCGCTCAGA GCGCTCTGTC
    ATCCTCATCA ACTTCTGTCT GTCCATCATC TCCTCCAATG CGCTTATACT CATCGGTCAA
    ACTCAGACAC GGAACAAGGT TTACCTGCTC TCGTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149429
    Clone ID Related Accession (Same CDS sequence) XM_021466980.1
    Accession Version XM_021466980.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4590bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product brain-specific angiogenesis inhibitor 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCA AAGGCCGGCT TGGCTCTCGT
    AGCCAATCCC TGCGCTCGGT GGACAGCCGT AAACGAGAGG ACTCTGATAA GACCCGCAGC
    GGACAGCAGT CTTCCCAAAC AGCCGCTCTG ATTACAGAAC ATGGGGCGTG GGGCGAGTGG
    ACACCATGGA GCTTGTGTTC ATCAACATGC GGCCGTGGCT ACAGGGAACG CAACCGCTCC
    TGCAAGCCAC CCCAGTTCGG AGGAAACCCC TGTGATGGTC CAGAGAAACA GACTAAATTC
    TGCAACATCG CTGTATGCCC AGTGGATGGC GTGTGGAACG AGTGGTCTGG TTGGAGTTCC
    TGTTCTTCAA CTTGTTCGAA TGGAACAATG CAGCGAATTC GGGAGTGTAA CGGGCCCTCG
    TATGGGGGCT TAGAGTGTCA AGGCGAGTGG CACGAGACAC GGGATTGCTT CCTTAGAGAA
    TGTCCTGTGG ATGGCCAATG GCAACTTTGG AGCACTTGGT CTGGTTGCAC CAAAACCTGT
    GGTGGAGGAA GTCAGCAGAG ACAGAGGCTG TGTTATGGTC CCTTTTTTGG GGGAGAGGCA
    TGCCCGGGGG ACCGAGAAGA AGTTAGACGT TGCAATGAGA AGCGATGCCC AGAGCCACAT
    GAAATCTGTG ATGAGGAGAA TTTCTCTAAT GTAGTGTGGA AGAAAACACC TGCAGGGGAC
    ACAGCGGCTG TCCGCTGCCC ACCAAATGCT GTAGGATTAA TTCTACGGCG ATGTTCATTA
    GACAATGAGG GTATAGCATA CTGGGAGAAC CCAACATATA TGAAGTGCGT CTCAAATGAT
    TACAGAAGTA TCCAAACACT GACCAGAGAT CACCTGTCCA AGGCGCAGCG AGGGCTTGTG
    GGGGATGGTG TTTCTGAAGT GATGACAAAG CTGAGGGTGA CGTCAAGTGA TGGAACGAGC
    TACAGCGGAG ATCTTTTGGC TATCATAGAT GTGCTAAAAA ACATGACAGA GATATTTAGG
    AGATCCTACT ACAGCCCCAG TAATGCAGAC ATGAGGAATT TTGTGCAGTC TGTGAGTAAC
    TTACTGATGG AGGAAAACAG GGACAGGTGG GAGGAAGCAC AACTGCTGGG ACCCAATGTT
    AAGGAGCTTT TTCGTCTAGT AGAGGACTTT GTGGACGTCA TCAGCCTTCG CATGAAGGAC
    TTCCAGGATA CTTATGAGGT CACGGATAAT CTAGTGTTGA GCATTCACAA GCGGCCCGTG
    GCTGGAAATG CAGATATCAC TTTCCCTATG AAGGGCTGGA GGGGGATGGT GGACTGGGCT
    CGCAGCTCTG AGGACAAAGT CAGAGTGGCC AAAAACATCC TGCTCACTGG AAAACCGGAA
    GAAGATGACG CTTCAGCTTT TGTGACTGGG ATCGTCCTGT ACAGAAACTT AGGCAGTATT
    CTCTCTCTGC AGAGAAATAG CACAGTGCTG AACTCCAAGG TGATCTCTGT GACAATCAAG
    CCCACTCCCA GCTCTCTCTC GAGTCCACTA GAGATTGAGT TCTCACACCT TTACAATGGA
    ACCACAAACC AAACCTGCAT TTCCTGGGAT GAGAACGACA GCTCGTCGTT GCTGGGCTCC
    TGGTCTGCGC GAGGCTGTAG AGCCGTGCCC GTCGACTCCA GCACAACCAA ATGCTTGTGT
    GACAGACTCT CCACCTTCGC CATTTTAGCA CGCCTCAATC CCGATGTGAA CATGGATAAG
    ACCCAGCTTC CATCTGTGAC TCTGATCGTG GGCTGCGGGG TCTCGTCCCT CACCCTGCTC
    CTCCTCATCA TCATCTATGT TTCTGTTTGG AGGTACATCC GCTCAGAGCG CTCTGTCATC
    CTCATCAACT TCTGTCTGTC CATCATCTCC TCCAATGCGC TTATACTCAT CGGTCAAACT
    CAGACACGGA ACAAGGTTGT TTGTACTCTC GTGGCTGCGT TTCTGCACTT TTTCTTCCTG
    TCCTCTTTCT GTTGGGTTCT AACGGAGGCA TGGCAGTCGT ACATGGCTGT GACCGGCCGC
    CTTAGAAACC GTATCATACG CAAGCGTTTC CTTTGCCTTG GTTGGGGTTT ACCTGCTCTC
    GTTGTAGCAA TTTCTGTTGG ATTCACCAAG GCAAAGGGAT ATGGCACTGT TAACTATTGC
    TGGCTGTCTC TGGAAGGAGG GCTGTTGTAC GCATTTGTGG GACCAGCTGC TGCTGTCGTC
    CTGGTTAACA TGGTTATCGG GATTCTGGTT TTTAACAAAC TGGTGTCCAA AGATGGCATT
    ACTGATATGA AACTCAAGGA GAGGGCCGGT CAGATGACAG TGCCACTGTA CAATATGACG
    CTCAAATGTG CCAAGTGCGG AGTTATCTCG TCGCCTGACG TTTCCACCAC AGCTACCAGT
    AACGCCATGG CGTCGCTGTG GAGCTCCTGC GTTGTTCTGC CGTTGCTGGC GCTCACTTGG
    ATGTCGGCAG TCTTGGCTAT TACTGACCGC CGTTCAGCCT TGTTTCAAAT CCTTTTTGCC
    GTCTTTGACT CCCTCGAGGG CTTTGTTATC GTGATGGTGC ATTGCATCCT ACGCAGAGAG
    GTTCAAGAAG CAGTAAAGTG TCGTGTTGTT GACCGTCAAG AAGATGGAAA CGGTGACTCT
    GGTGGATCCT TCCAGAACGG TCATGCTCAG CTGATGACTG ACTTTGAAAA GGATGTGGAC
    ATAGCTTGTC GATCAGGCAC CCTGAAGCGT CCCTCCCAGC AAGGCGAGGA GAAGGTTCCA
    TCCCAGCAGA TCACAGTACA GAAAGGCTCC AACTTCAACA CCCTTCCTGC CAGCATGGCA
    AAGGTCCACC TGCAAAACGT AGCCAACTAC GCCAGTCACA CTCTCACCAT GCGCCGCGAC
    AAAGGCGCCA TCGCCGGGGT CAGCACGGAG TTACCCGGGG CCAAATCTAT TTACATCTGT
    GACGGCGAGC TCTTCAAACA GCTTGACGGA GCACCGGGAG ACATTCCTGA AGGAGGTGGT
    GGAGGTGCAG GTCAGGGTTA TGTGCTTTTG CCCAACAACA ACAACACCCT TAGGCCTACG
    AAGGGTGGCA AGGAAGACCC GGCCGCCAAA TATAACATCA GTATCGAACA GTTACCCCAG
    ACAAGGCTGA TCCACCTGGC CAATCCTGTT GGCACCGATC CCGTGGCAGG CTTCGGGCTG
    AAGAGCTTGC CCACCGACCG AGTCAGCGTT TCATGCTCGG AGAGGGAGTC CCCGGTGCAG
    ACGGTGCAGA ATATCTCCAG CGAGTCCCAG ATGACAAATA CGTGTGAGCA AGGGGATTCT
    GGGAACTCTG GGATGTCTAA GAGCGAGACT GTCTCCACAC TGTCCATGAG CTCTCTGGAG
    AGACGAAAAT CACGCTATGC AGAGCTGGAC TTTGAGAAGA TCATGCACAC ACGGAAGCGT
    CATCAGGACA TGTTTCAGGA CCTGAATCGC AAACTGCAGC ACGCAGAGAA GGACAGAGAC
    TCTCCTCCAG TCGACTGCAA GCCTGGAAAG AGATGGAGCA TTTCGTCAGG AGGAAGCGAC
    AAAACCAACC TTAGTGACAA GCAGCAGACC CCCAGTAAGC GGGCATGGGA GGGCATCAGG
    AAGACTCACT CTCCTCCATC ATGGGTGCGC AAGGAGCTGG AGCCGCTGCA GGCCTCACCG
    CTGGAGATGC AGGCAGTGGA GTGGGAGAAG GCCAGTGCCA CCATCCCTCT AGTGGGCCAG
    GAGATCATGG ACCTGCAGAC GGAGGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322655.1
    CDS747..5336
    Translation

    Target ORF information:

    RefSeq Version XM_021466980.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466980.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCA AAGGCCGGCT TGGCTCTCGT
    AGCCAATCCC TGCGCTCGGT GGACAGCCGT AAACGAGAGG ACTCTGATAA GACCCGCAGC
    GGACAGCAGT CTTCCCAAAC AGCCGCTCTG ATTACAGAAC ATGGGGCGTG GGGCGAGTGG
    ACACCATGGA GCTTGTGTTC ATCAACATGC GGCCGTGGCT ACAGGGAACG CAACCGCTCC
    TGCAAGCCAC CCCAGTTCGG AGGAAACCCC TGTGATGGTC CAGAGAAACA GACTAAATTC
    TGCAACATCG CTGTATGCCC AGTGGATGGC GTGTGGAACG AGTGGTCTGG TTGGAGTTCC
    TGTTCTTCAA CTTGTTCGAA TGGAACAATG CAGCGAATTC GGGAGTGTAA CGGGCCCTCG
    TATGGGGGCT TAGAGTGTCA AGGCGAGTGG CACGAGACAC GGGATTGCTT CCTTAGAGAA
    TGTCCTGTGG ATGGCCAATG GCAACTTTGG AGCACTTGGT CTGGTTGCAC CAAAACCTGT
    GGTGGAGGAA GTCAGCAGAG ACAGAGGCTG TGTTATGGTC CCTTTTTTGG GGGAGAGGCA
    TGCCCGGGGG ACCGAGAAGA AGTTAGACGT TGCAATGAGA AGCGATGCCC AGAGCCACAT
    GAAATCTGTG ATGAGGAGAA TTTCTCTAAT GTAGTGTGGA AGAAAACACC TGCAGGGGAC
    ACAGCGGCTG TCCGCTGCCC ACCAAATGCT GTAGGATTAA TTCTACGGCG ATGTTCATTA
    GACAATGAGG GTATAGCATA CTGGGAGAAC CCAACATATA TGAAGTGCGT CTCAAATGAT
    TACAGAAGTA TCCAAACACT GACCAGAGAT CACCTGTCCA AGGCGCAGCG AGGGCTTGTG
    GGGGATGGTG TTTCTGAAGT GATGACAAAG CTGAGGGTGA CGTCAAGTGA TGGAACGAGC
    TACAGCGGAG ATCTTTTGGC TATCATAGAT GTGCTAAAAA ACATGACAGA GATATTTAGG
    AGATCCTACT ACAGCCCCAG TAATGCAGAC ATGAGGAATT TTGTGCAGTC TGTGAGTAAC
    TTACTGATGG AGGAAAACAG GGACAGGTGG GAGGAAGCAC AACTGCTGGG ACCCAATGTT
    AAGGAGCTTT TTCGTCTAGT AGAGGACTTT GTGGACGTCA TCAGCCTTCG CATGAAGGAC
    TTCCAGGATA CTTATGAGGT CACGGATAAT CTAGTGTTGA GCATTCACAA GCGGCCCGTG
    GCTGGAAATG CAGATATCAC TTTCCCTATG AAGGGCTGGA GGGGGATGGT GGACTGGGCT
    CGCAGCTCTG AGGACAAAGT CAGAGTGGCC AAAAACATCC TGCTCACTGG AAAACCGGAA
    GAAGATGACG CTTCAGCTTT TGTGACTGGG ATCGTCCTGT ACAGAAACTT AGGCAGTATT
    CTCTCTCTGC AGAGAAATAG CACAGTGCTG AACTCCAAGG TGATCTCTGT GACAATCAAG
    CCCACTCCCA GCTCTCTCTC GAGTCCACTA GAGATTGAGT TCTCACACCT TTACAATGGA
    ACCACAAACC AAACCTGCAT TTCCTGGGAT GAGAACGACA GCTCGTCGTT GCTGGGCTCC
    TGGTCTGCGC GAGGCTGTAG AGCCGTGCCC GTCGACTCCA GCACAACCAA ATGCTTGTGT
    GACAGACTCT CCACCTTCGC CATTTTAGCA CGCCTCAATC CCGATGTGAA CATGGATAAG
    ACCCAGCTTC CATCTGTGAC TCTGATCGTG GGCTGCGGGG TCTCGTCCCT CACCCTGCTC
    CTCCTCATCA TCATCTATGT TTCTGTTTGG AGGTACATCC GCTCAGAGCG CTCTGTCATC
    CTCATCAACT TCTGTCTGTC CATCATCTCC TCCAATGCGC TTATACTCAT CGGTCAAACT
    CAGACACGGA ACAAGGTTGT TTGTACTCTC GTGGCTGCGT TTCTGCACTT TTTCTTCCTG
    TCCTCTTTCT GTTGGGTTCT AACGGAGGCA TGGCAGTCGT ACATGGCTGT GACCGGCCGC
    CTTAGAAACC GTATCATACG CAAGCGTTTC CTTTGCCTTG GTTGGGGTTT ACCTGCTCTC
    GTTGTAGCAA TTTCTGTTGG ATTCACCAAG GCAAAGGGAT ATGGCACTGT TAACTATTGC
    TGGCTGTCTC TGGAAGGAGG GCTGTTGTAC GCATTTGTGG GACCAGCTGC TGCTGTCGTC
    CTGGTTAACA TGGTTATCGG GATTCTGGTT TTTAACAAAC TGGTGTCCAA AGATGGCATT
    ACTGATATGA AACTCAAGGA GAGGGCCGGT CAGATGACAG TGCCACTGTA CAATATGACG
    CTCAAATGTG CCAAGTGCGG AGTTATCTCG TCGCCTGACG TTTCCACCAC AGCTACCAGT
    AACGCCATGG CGTCGCTGTG GAGCTCCTGC GTTGTTCTGC CGTTGCTGGC GCTCACTTGG
    ATGTCGGCAG TCTTGGCTAT TACTGACCGC CGTTCAGCCT TGTTTCAAAT CCTTTTTGCC
    GTCTTTGACT CCCTCGAGGG CTTTGTTATC GTGATGGTGC ATTGCATCCT ACGCAGAGAG
    GTTCAAGAAG CAGTAAAGTG TCGTGTTGTT GACCGTCAAG AAGATGGAAA CGGTGACTCT
    GGTGGATCCT TCCAGAACGG TCATGCTCAG CTGATGACTG ACTTTGAAAA GGATGTGGAC
    ATAGCTTGTC GATCAGGCAC CCTGAAGCGT CCCTCCCAGC AAGGCGAGGA GAAGGTTCCA
    TCCCAGCAGA TCACAGTACA GAAAGGCTCC AACTTCAACA CCCTTCCTGC CAGCATGGCA
    AAGGTCCACC TGCAAAACGT AGCCAACTAC GCCAGTCACA CTCTCACCAT GCGCCGCGAC
    AAAGGCGCCA TCGCCGGGGT CAGCACGGAG TTACCCGGGG CCAAATCTAT TTACATCTGT
    GACGGCGAGC TCTTCAAACA GCTTGACGGA GCACCGGGAG ACATTCCTGA AGGAGGTGGT
    GGAGGTGCAG GTCAGGGTTA TGTGCTTTTG CCCAACAACA ACAACACCCT TAGGCCTACG
    AAGGGTGGCA AGGAAGACCC GGCCGCCAAA TATAACATCA GTATCGAACA GTTACCCCAG
    ACAAGGCTGA TCCACCTGGC CAATCCTGTT GGCACCGATC CCGTGGCAGG CTTCGGGCTG
    AAGAGCTTGC CCACCGACCG AGTCAGCGTT TCATGCTCGG AGAGGGAGTC CCCGGTGCAG
    ACGGTGCAGA ATATCTCCAG CGAGTCCCAG ATGACAAATA CGTGTGAGCA AGGGGATTCT
    GGGAACTCTG GGATGTCTAA GAGCGAGACT GTCTCCACAC TGTCCATGAG CTCTCTGGAG
    AGACGAAAAT CACGCTATGC AGAGCTGGAC TTTGAGAAGA TCATGCACAC ACGGAAGCGT
    CATCAGGACA TGTTTCAGGA CCTGAATCGC AAACTGCAGC ACGCAGAGAA GGACAGAGAC
    TCTCCTCCAG TCGACTGCAA GCCTGGAAAG AGATGGAGCA TTTCGTCAGG AGGAAGCGAC
    AAAACCAACC TTAGTGACAA GCAGCAGACC CCCAGTAAGC GGGCATGGGA GGGCATCAGG
    AAGACTCACT CTCCTCCATC ATGGGTGCGC AAGGAGCTGG AGCCGCTGCA GGCCTCACCG
    CTGGAGATGC AGGCAGTGGA GTGGGAGAAG GCCAGTGCCA CCATCCCTCT AGTGGGCCAG
    GAGATCATGG ACCTGCAGAC GGAGGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149430
    Clone ID Related Accession (Same CDS sequence) XM_021466981.1
    Accession Version XM_021466981.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4560bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product brain-specific angiogenesis inhibitor 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCT ACAGGCAGTA TTCCTCAAAA
    GGCCAGGGTT TAAGTCCAGT GAAATACAAA GGAATGCAAG AGGCCATGCT TGCCGCTCTG
    ATTACAGAAC ATGGGGCGTG GGGCGAGTGG ACACCATGGA GCTTGTGTTC ATCAACATGC
    GGCCGTGGCT ACAGGGAACG CAACCGCTCC TGCAAGCCAC CCCAGTTCGG AGGAAACCCC
    TGTGATGGTC CAGAGAAACA GACTAAATTC TGCAACATCG CTGTATGCCC AGTGGATGGC
    GTGTGGAACG AGTGGTCTGG TTGGAGTTCC TGTTCTTCAA CTTGTTCGAA TGGAACAATG
    CAGCGAATTC GGGAGTGTAA CGGGCCCTCG TATGGGGGCT TAGAGTGTCA AGGCGAGTGG
    CACGAGACAC GGGATTGCTT CCTTAGAGAA TGTCCTGTGG ATGGCCAATG GCAACTTTGG
    AGCACTTGGT CTGGTTGCAC CAAAACCTGT GGTGGAGGAA GTCAGCAGAG ACAGAGGCTG
    TGTTATGGTC CCTTTTTTGG GGGAGAGGCA TGCCCGGGGG ACCGAGAAGA AGTTAGACGT
    TGCAATGAGA AGCGATGCCC AGAGCCACAT GAAATCTGTG ATGAGGAGAA TTTCTCTAAT
    GTAGTGTGGA AGAAAACACC TGCAGGGGAC ACAGCGGCTG TCCGCTGCCC ACCAAATGCT
    GTAGGATTAA TTCTACGGCG ATGTTCATTA GACAATGAGG GTATAGCATA CTGGGAGAAC
    CCAACATATA TGAAGTGCGT CTCAAATGAT TACAGAAGTA TCCAAACACT GACCAGAGAT
    CACCTGTCCA AGGCGCAGCG AGGGCTTGTG GGGGATGGTG TTTCTGAAGT GATGACAAAG
    CTGAGGGTGA CGTCAAGTGA TGGAACGAGC TACAGCGGAG ATCTTTTGGC TATCATAGAT
    GTGCTAAAAA ACATGACAGA GATATTTAGG AGATCCTACT ACAGCCCCAG TAATGCAGAC
    ATGAGGAATT TTGTGCAGTC TGTGAGTAAC TTACTGATGG AGGAAAACAG GGACAGGTGG
    GAGGAAGCAC AACTGCTGGG ACCCAATGTT AAGGAGCTTT TTCGTCTAGT AGAGGACTTT
    GTGGACGTCA TCAGCCTTCG CATGAAGGAC TTCCAGGATA CTTATGAGGT CACGGATAAT
    CTAGTGTTGA GCATTCACAA GCGGCCCGTG GCTGGAAATG CAGATATCAC TTTCCCTATG
    AAGGGCTGGA GGGGGATGGT GGACTGGGCT CGCAGCTCTG AGGACAAAGT CAGAGTGGCC
    AAAAACATCC TGCTCACTGG AAAACCGGAA GAAGATGACG CTTCAGCTTT TGTGACTGGG
    ATCGTCCTGT ACAGAAACTT AGGCAGTATT CTCTCTCTGC AGAGAAATAG CACAGTGCTG
    AACTCCAAGG TGATCTCTGT GACAATCAAG CCCACTCCCA GCTCTCTCTC GAGTCCACTA
    GAGATTGAGT TCTCACACCT TTACAATGGA ACCACAAACC AAACCTGCAT TTCCTGGGAT
    GAGAACGACA GCTCGTCGTT GCTGGGCTCC TGGTCTGCGC GAGGCTGTAG AGCCGTGCCC
    GTCGACTCCA GCACAACCAA ATGCTTGTGT GACAGACTCT CCACCTTCGC CATTTTAGCA
    CGCCTCAATC CCGATGTGAA CATGGATAAG ACCCAGCTTC CATCTGTGAC TCTGATCGTG
    GGCTGCGGGG TCTCGTCCCT CACCCTGCTC CTCCTCATCA TCATCTATGT TTCTGTTTGG
    AGGTACATCC GCTCAGAGCG CTCTGTCATC CTCATCAACT TCTGTCTGTC CATCATCTCC
    TCCAATGCGC TTATACTCAT CGGTCAAACT CAGACACGGA ACAAGGTTGT TTGTACTCTC
    GTGGCTGCGT TTCTGCACTT TTTCTTCCTG TCCTCTTTCT GTTGGGTTCT AACGGAGGCA
    TGGCAGTCGT ACATGGCTGT GACCGGCCGC CTTAGAAACC GTATCATACG CAAGCGTTTC
    CTTTGCCTTG GTTGGGGTTT ACCTGCTCTC GTTGTAGCAA TTTCTGTTGG ATTCACCAAG
    GCAAAGGGAT ATGGCACTGT TAACTATTGC TGGCTGTCTC TGGAAGGAGG GCTGTTGTAC
    GCATTTGTGG GACCAGCTGC TGCTGTCGTC CTGGTTAACA TGGTTATCGG GATTCTGGTT
    TTTAACAAAC TGGTGTCCAA AGATGGCATT ACTGATATGA AACTCAAGGA GAGGGCCGGT
    CAGATGACAG TGCCACTGTA CAATATGACG CTCAAATGTG CCAAGTGCGG AGTTATCTCG
    TCGCCTGACG TTTCCACCAC AGCTACCAGT AACGCCATGG CGTCGCTGTG GAGCTCCTGC
    GTTGTTCTGC CGTTGCTGGC GCTCACTTGG ATGTCGGCAG TCTTGGCTAT TACTGACCGC
    CGTTCAGCCT TGTTTCAAAT CCTTTTTGCC GTCTTTGACT CCCTCGAGGG CTTTGTTATC
    GTGATGGTGC ATTGCATCCT ACGCAGAGAG GTTCAAGAAG CAGTAAAGTG TCGTGTTGTT
    GACCGTCAAG AAGATGGAAA CGGTGACTCT GGTGGATCCT TCCAGAACGG TCATGCTCAG
    CTGATGACTG ACTTTGAAAA GGATGTGGAC ATAGCTTGTC GATCAGGCAC CCTGAAGCGT
    CCCTCCCAGC AAGGCGAGGA GAAGGTTCCA TCCCAGCAGA TCACAGTACA GAAAGGCTCC
    AACTTCAACA CCCTTCCTGC CAGCATGGCA AAGGTCCACC TGCAAAACGT AGCCAACTAC
    GCCAGTCACA CTCTCACCAT GCGCCGCGAC AAAGGCGCCA TCGCCGGGGT CAGCACGGAG
    TTACCCGGGG CCAAATCTAT TTACATCTGT GACGGCGAGC TCTTCAAACA GCTTGACGGA
    GCACCGGGAG ACATTCCTGA AGGAGGTGGT GGAGGTGCAG GTCAGGGTTA TGTGCTTTTG
    CCCAACAACA ACAACACCCT TAGGCCTACG AAGGGTGGCA AGGAAGACCC GGCCGCCAAA
    TATAACATCA GTATCGAACA GTTACCCCAG ACAAGGCTGA TCCACCTGGC CAATCCTGTT
    GGCACCGATC CCGTGGCAGG CTTCGGGCTG AAGAGCTTGC CCACCGACCG AGTCAGCGTT
    TCATGCTCGG AGAGGGAGTC CCCGGTGCAG ACGGTGCAGA ATATCTCCAG CGAGTCCCAG
    ATGACAAATA CGTGTGAGCA AGGGGATTCT GGGAACTCTG GGATGTCTAA GAGCGAGACT
    GTCTCCACAC TGTCCATGAG CTCTCTGGAG AGACGAAAAT CACGCTATGC AGAGCTGGAC
    TTTGAGAAGA TCATGCACAC ACGGAAGCGT CATCAGGACA TGTTTCAGGA CCTGAATCGC
    AAACTGCAGC ACGCAGAGAA GGACAGAGAC TCTCCTCCAG TCGACTGCAA GCCTGGAAAG
    AGATGGAGCA TTTCGTCAGG AGGAAGCGAC AAAACCAACC TTAGTGACAA GCAGCAGACC
    CCCAGTAAGC GGGCATGGGA GGGCATCAGG AAGACTCACT CTCCTCCATC ATGGGTGCGC
    AAGGAGCTGG AGCCGCTGCA GGCCTCACCG CTGGAGATGC AGGCAGTGGA GTGGGAGAAG
    GCCAGTGCCA CCATCCCTCT AGTGGGCCAG GAGATCATGG ACCTGCAGAC GGAGGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322656.1
    CDS747..5306
    Translation

    Target ORF information:

    RefSeq Version XM_021466981.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466981.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCT ACAGGCAGTA TTCCTCAAAA
    GGCCAGGGTT TAAGTCCAGT GAAATACAAA GGAATGCAAG AGGCCATGCT TGCCGCTCTG
    ATTACAGAAC ATGGGGCGTG GGGCGAGTGG ACACCATGGA GCTTGTGTTC ATCAACATGC
    GGCCGTGGCT ACAGGGAACG CAACCGCTCC TGCAAGCCAC CCCAGTTCGG AGGAAACCCC
    TGTGATGGTC CAGAGAAACA GACTAAATTC TGCAACATCG CTGTATGCCC AGTGGATGGC
    GTGTGGAACG AGTGGTCTGG TTGGAGTTCC TGTTCTTCAA CTTGTTCGAA TGGAACAATG
    CAGCGAATTC GGGAGTGTAA CGGGCCCTCG TATGGGGGCT TAGAGTGTCA AGGCGAGTGG
    CACGAGACAC GGGATTGCTT CCTTAGAGAA TGTCCTGTGG ATGGCCAATG GCAACTTTGG
    AGCACTTGGT CTGGTTGCAC CAAAACCTGT GGTGGAGGAA GTCAGCAGAG ACAGAGGCTG
    TGTTATGGTC CCTTTTTTGG GGGAGAGGCA TGCCCGGGGG ACCGAGAAGA AGTTAGACGT
    TGCAATGAGA AGCGATGCCC AGAGCCACAT GAAATCTGTG ATGAGGAGAA TTTCTCTAAT
    GTAGTGTGGA AGAAAACACC TGCAGGGGAC ACAGCGGCTG TCCGCTGCCC ACCAAATGCT
    GTAGGATTAA TTCTACGGCG ATGTTCATTA GACAATGAGG GTATAGCATA CTGGGAGAAC
    CCAACATATA TGAAGTGCGT CTCAAATGAT TACAGAAGTA TCCAAACACT GACCAGAGAT
    CACCTGTCCA AGGCGCAGCG AGGGCTTGTG GGGGATGGTG TTTCTGAAGT GATGACAAAG
    CTGAGGGTGA CGTCAAGTGA TGGAACGAGC TACAGCGGAG ATCTTTTGGC TATCATAGAT
    GTGCTAAAAA ACATGACAGA GATATTTAGG AGATCCTACT ACAGCCCCAG TAATGCAGAC
    ATGAGGAATT TTGTGCAGTC TGTGAGTAAC TTACTGATGG AGGAAAACAG GGACAGGTGG
    GAGGAAGCAC AACTGCTGGG ACCCAATGTT AAGGAGCTTT TTCGTCTAGT AGAGGACTTT
    GTGGACGTCA TCAGCCTTCG CATGAAGGAC TTCCAGGATA CTTATGAGGT CACGGATAAT
    CTAGTGTTGA GCATTCACAA GCGGCCCGTG GCTGGAAATG CAGATATCAC TTTCCCTATG
    AAGGGCTGGA GGGGGATGGT GGACTGGGCT CGCAGCTCTG AGGACAAAGT CAGAGTGGCC
    AAAAACATCC TGCTCACTGG AAAACCGGAA GAAGATGACG CTTCAGCTTT TGTGACTGGG
    ATCGTCCTGT ACAGAAACTT AGGCAGTATT CTCTCTCTGC AGAGAAATAG CACAGTGCTG
    AACTCCAAGG TGATCTCTGT GACAATCAAG CCCACTCCCA GCTCTCTCTC GAGTCCACTA
    GAGATTGAGT TCTCACACCT TTACAATGGA ACCACAAACC AAACCTGCAT TTCCTGGGAT
    GAGAACGACA GCTCGTCGTT GCTGGGCTCC TGGTCTGCGC GAGGCTGTAG AGCCGTGCCC
    GTCGACTCCA GCACAACCAA ATGCTTGTGT GACAGACTCT CCACCTTCGC CATTTTAGCA
    CGCCTCAATC CCGATGTGAA CATGGATAAG ACCCAGCTTC CATCTGTGAC TCTGATCGTG
    GGCTGCGGGG TCTCGTCCCT CACCCTGCTC CTCCTCATCA TCATCTATGT TTCTGTTTGG
    AGGTACATCC GCTCAGAGCG CTCTGTCATC CTCATCAACT TCTGTCTGTC CATCATCTCC
    TCCAATGCGC TTATACTCAT CGGTCAAACT CAGACACGGA ACAAGGTTGT TTGTACTCTC
    GTGGCTGCGT TTCTGCACTT TTTCTTCCTG TCCTCTTTCT GTTGGGTTCT AACGGAGGCA
    TGGCAGTCGT ACATGGCTGT GACCGGCCGC CTTAGAAACC GTATCATACG CAAGCGTTTC
    CTTTGCCTTG GTTGGGGTTT ACCTGCTCTC GTTGTAGCAA TTTCTGTTGG ATTCACCAAG
    GCAAAGGGAT ATGGCACTGT TAACTATTGC TGGCTGTCTC TGGAAGGAGG GCTGTTGTAC
    GCATTTGTGG GACCAGCTGC TGCTGTCGTC CTGGTTAACA TGGTTATCGG GATTCTGGTT
    TTTAACAAAC TGGTGTCCAA AGATGGCATT ACTGATATGA AACTCAAGGA GAGGGCCGGT
    CAGATGACAG TGCCACTGTA CAATATGACG CTCAAATGTG CCAAGTGCGG AGTTATCTCG
    TCGCCTGACG TTTCCACCAC AGCTACCAGT AACGCCATGG CGTCGCTGTG GAGCTCCTGC
    GTTGTTCTGC CGTTGCTGGC GCTCACTTGG ATGTCGGCAG TCTTGGCTAT TACTGACCGC
    CGTTCAGCCT TGTTTCAAAT CCTTTTTGCC GTCTTTGACT CCCTCGAGGG CTTTGTTATC
    GTGATGGTGC ATTGCATCCT ACGCAGAGAG GTTCAAGAAG CAGTAAAGTG TCGTGTTGTT
    GACCGTCAAG AAGATGGAAA CGGTGACTCT GGTGGATCCT TCCAGAACGG TCATGCTCAG
    CTGATGACTG ACTTTGAAAA GGATGTGGAC ATAGCTTGTC GATCAGGCAC CCTGAAGCGT
    CCCTCCCAGC AAGGCGAGGA GAAGGTTCCA TCCCAGCAGA TCACAGTACA GAAAGGCTCC
    AACTTCAACA CCCTTCCTGC CAGCATGGCA AAGGTCCACC TGCAAAACGT AGCCAACTAC
    GCCAGTCACA CTCTCACCAT GCGCCGCGAC AAAGGCGCCA TCGCCGGGGT CAGCACGGAG
    TTACCCGGGG CCAAATCTAT TTACATCTGT GACGGCGAGC TCTTCAAACA GCTTGACGGA
    GCACCGGGAG ACATTCCTGA AGGAGGTGGT GGAGGTGCAG GTCAGGGTTA TGTGCTTTTG
    CCCAACAACA ACAACACCCT TAGGCCTACG AAGGGTGGCA AGGAAGACCC GGCCGCCAAA
    TATAACATCA GTATCGAACA GTTACCCCAG ACAAGGCTGA TCCACCTGGC CAATCCTGTT
    GGCACCGATC CCGTGGCAGG CTTCGGGCTG AAGAGCTTGC CCACCGACCG AGTCAGCGTT
    TCATGCTCGG AGAGGGAGTC CCCGGTGCAG ACGGTGCAGA ATATCTCCAG CGAGTCCCAG
    ATGACAAATA CGTGTGAGCA AGGGGATTCT GGGAACTCTG GGATGTCTAA GAGCGAGACT
    GTCTCCACAC TGTCCATGAG CTCTCTGGAG AGACGAAAAT CACGCTATGC AGAGCTGGAC
    TTTGAGAAGA TCATGCACAC ACGGAAGCGT CATCAGGACA TGTTTCAGGA CCTGAATCGC
    AAACTGCAGC ACGCAGAGAA GGACAGAGAC TCTCCTCCAG TCGACTGCAA GCCTGGAAAG
    AGATGGAGCA TTTCGTCAGG AGGAAGCGAC AAAACCAACC TTAGTGACAA GCAGCAGACC
    CCCAGTAAGC GGGCATGGGA GGGCATCAGG AAGACTCACT CTCCTCCATC ATGGGTGCGC
    AAGGAGCTGG AGCCGCTGCA GGCCTCACCG CTGGAGATGC AGGCAGTGGA GTGGGAGAAG
    GCCAGTGCCA CCATCCCTCT AGTGGGCCAG GAGATCATGG ACCTGCAGAC GGAGGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149431
    Clone ID Related Accession (Same CDS sequence) XM_021466982.1
    Accession Version XM_021466982.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4545bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product brain-specific angiogenesis inhibitor 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCT ACAGGCAGTA TTCCTCAAAA
    GGCCAGGGTT TAAGTCCAGT GAAATACAAA GGAATGCAAG AGGCCATGCT TGAACATGGG
    GCGTGGGGCG AGTGGACACC ATGGAGCTTG TGTTCATCAA CATGCGGCCG TGGCTACAGG
    GAACGCAACC GCTCCTGCAA GCCACCCCAG TTCGGAGGAA ACCCCTGTGA TGGTCCAGAG
    AAACAGACTA AATTCTGCAA CATCGCTGTA TGCCCAGTGG ATGGCGTGTG GAACGAGTGG
    TCTGGTTGGA GTTCCTGTTC TTCAACTTGT TCGAATGGAA CAATGCAGCG AATTCGGGAG
    TGTAACGGGC CCTCGTATGG GGGCTTAGAG TGTCAAGGCG AGTGGCACGA GACACGGGAT
    TGCTTCCTTA GAGAATGTCC TGTGGATGGC CAATGGCAAC TTTGGAGCAC TTGGTCTGGT
    TGCACCAAAA CCTGTGGTGG AGGAAGTCAG CAGAGACAGA GGCTGTGTTA TGGTCCCTTT
    TTTGGGGGAG AGGCATGCCC GGGGGACCGA GAAGAAGTTA GACGTTGCAA TGAGAAGCGA
    TGCCCAGAGC CACATGAAAT CTGTGATGAG GAGAATTTCT CTAATGTAGT GTGGAAGAAA
    ACACCTGCAG GGGACACAGC GGCTGTCCGC TGCCCACCAA ATGCTGTAGG ATTAATTCTA
    CGGCGATGTT CATTAGACAA TGAGGGTATA GCATACTGGG AGAACCCAAC ATATATGAAG
    TGCGTCTCAA ATGATTACAG AAGTATCCAA ACACTGACCA GAGATCACCT GTCCAAGGCG
    CAGCGAGGGC TTGTGGGGGA TGGTGTTTCT GAAGTGATGA CAAAGCTGAG GGTGACGTCA
    AGTGATGGAA CGAGCTACAG CGGAGATCTT TTGGCTATCA TAGATGTGCT AAAAAACATG
    ACAGAGATAT TTAGGAGATC CTACTACAGC CCCAGTAATG CAGACATGAG GAATTTTGTG
    CAGTCTGTGA GTAACTTACT GATGGAGGAA AACAGGGACA GGTGGGAGGA AGCACAACTG
    CTGGGACCCA ATGTTAAGGA GCTTTTTCGT CTAGTAGAGG ACTTTGTGGA CGTCATCAGC
    CTTCGCATGA AGGACTTCCA GGATACTTAT GAGGTCACGG ATAATCTAGT GTTGAGCATT
    CACAAGCGGC CCGTGGCTGG AAATGCAGAT ATCACTTTCC CTATGAAGGG CTGGAGGGGG
    ATGGTGGACT GGGCTCGCAG CTCTGAGGAC AAAGTCAGAG TGGCCAAAAA CATCCTGCTC
    ACTGGAAAAC CGGAAGAAGA TGACGCTTCA GCTTTTGTGA CTGGGATCGT CCTGTACAGA
    AACTTAGGCA GTATTCTCTC TCTGCAGAGA AATAGCACAG TGCTGAACTC CAAGGTGATC
    TCTGTGACAA TCAAGCCCAC TCCCAGCTCT CTCTCGAGTC CACTAGAGAT TGAGTTCTCA
    CACCTTTACA ATGGAACCAC AAACCAAACC TGCATTTCCT GGGATGAGAA CGACAGCTCG
    TCGTTGCTGG GCTCCTGGTC TGCGCGAGGC TGTAGAGCCG TGCCCGTCGA CTCCAGCACA
    ACCAAATGCT TGTGTGACAG ACTCTCCACC TTCGCCATTT TAGCACGCCT CAATCCCGAT
    GTGAACATGG ATAAGACCCA GCTTCCATCT GTGACTCTGA TCGTGGGCTG CGGGGTCTCG
    TCCCTCACCC TGCTCCTCCT CATCATCATC TATGTTTCTG TTTGGAGGTA CATCCGCTCA
    GAGCGCTCTG TCATCCTCAT CAACTTCTGT CTGTCCATCA TCTCCTCCAA TGCGCTTATA
    CTCATCGGTC AAACTCAGAC ACGGAACAAG GTTGTTTGTA CTCTCGTGGC TGCGTTTCTG
    CACTTTTTCT TCCTGTCCTC TTTCTGTTGG GTTCTAACGG AGGCATGGCA GTCGTACATG
    GCTGTGACCG GCCGCCTTAG AAACCGTATC ATACGCAAGC GTTTCCTTTG CCTTGGTTGG
    GGTTTACCTG CTCTCGTTGT AGCAATTTCT GTTGGATTCA CCAAGGCAAA GGGATATGGC
    ACTGTTAACT ATTGCTGGCT GTCTCTGGAA GGAGGGCTGT TGTACGCATT TGTGGGACCA
    GCTGCTGCTG TCGTCCTGGT TAACATGGTT ATCGGGATTC TGGTTTTTAA CAAACTGGTG
    TCCAAAGATG GCATTACTGA TATGAAACTC AAGGAGAGGG CCGGTCAGAT GACAGTGCCA
    CTGTACAATA TGACGCTCAA ATGTGCCAAG TGCGGAGTTA TCTCGTCGCC TGACGTTTCC
    ACCACAGCTA CCAGTAACGC CATGGCGTCG CTGTGGAGCT CCTGCGTTGT TCTGCCGTTG
    CTGGCGCTCA CTTGGATGTC GGCAGTCTTG GCTATTACTG ACCGCCGTTC AGCCTTGTTT
    CAAATCCTTT TTGCCGTCTT TGACTCCCTC GAGGGCTTTG TTATCGTGAT GGTGCATTGC
    ATCCTACGCA GAGAGGTTCA AGAAGCAGTA AAGTGTCGTG TTGTTGACCG TCAAGAAGAT
    GGAAACGGTG ACTCTGGTGG ATCCTTCCAG AACGGTCATG CTCAGCTGAT GACTGACTTT
    GAAAAGGATG TGGACATAGC TTGTCGATCA GGCACCCTGA AGCGTCCCTC CCAGCAAGGC
    GAGGAGAAGG TTCCATCCCA GCAGATCACA GTACAGAAAG GCTCCAACTT CAACACCCTT
    CCTGCCAGCA TGGCAAAGGT CCACCTGCAA AACGTAGCCA ACTACGCCAG TCACACTCTC
    ACCATGCGCC GCGACAAAGG CGCCATCGCC GGGGTCAGCA CGGAGTTACC CGGGGCCAAA
    TCTATTTACA TCTGTGACGG CGAGCTCTTC AAACAGCTTG ACGGAGCACC GGGAGACATT
    CCTGAAGGAG GTGGTGGAGG TGCAGGTCAG GGTTATGTGC TTTTGCCCAA CAACAACAAC
    ACCCTTAGGC CTACGAAGGG TGGCAAGGAA GACCCGGCCG CCAAATATAA CATCAGTATC
    GAACAGTTAC CCCAGACAAG GCTGATCCAC CTGGCCAATC CTGTTGGCAC CGATCCCGTG
    GCAGGCTTCG GGCTGAAGAG CTTGCCCACC GACCGAGTCA GCGTTTCATG CTCGGAGAGG
    GAGTCCCCGG TGCAGACGGT GCAGAATATC TCCAGCGAGT CCCAGATGAC AAATACGTGT
    GAGCAAGGGG ATTCTGGGAA CTCTGGGATG TCTAAGAGCG AGACTGTCTC CACACTGTCC
    ATGAGCTCTC TGGAGAGACG AAAATCACGC TATGCAGAGC TGGACTTTGA GAAGATCATG
    CACACACGGA AGCGTCATCA GGACATGTTT CAGGACCTGA ATCGCAAACT GCAGCACGCA
    GAGAAGGACA GAGACTCTCC TCCAGTCGAC TGCAAGCCTG GAAAGAGATG GAGCATTTCG
    TCAGGAGGAA GCGACAAAAC CAACCTTAGT GACAAGCAGC AGACCCCCAG TAAGCGGGCA
    TGGGAGGGCA TCAGGAAGAC TCACTCTCCT CCATCATGGG TGCGCAAGGA GCTGGAGCCG
    CTGCAGGCCT CACCGCTGGA GATGCAGGCA GTGGAGTGGG AGAAGGCCAG TGCCACCATC
    CCTCTAGTGG GCCAGGAGAT CATGGACCTG CAGACGGAGG TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322657.1
    CDS747..5291
    Translation

    Target ORF information:

    RefSeq Version XM_021466982.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466982.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCT ACAGGCAGTA TTCCTCAAAA
    GGCCAGGGTT TAAGTCCAGT GAAATACAAA GGAATGCAAG AGGCCATGCT TGAACATGGG
    GCGTGGGGCG AGTGGACACC ATGGAGCTTG TGTTCATCAA CATGCGGCCG TGGCTACAGG
    GAACGCAACC GCTCCTGCAA GCCACCCCAG TTCGGAGGAA ACCCCTGTGA TGGTCCAGAG
    AAACAGACTA AATTCTGCAA CATCGCTGTA TGCCCAGTGG ATGGCGTGTG GAACGAGTGG
    TCTGGTTGGA GTTCCTGTTC TTCAACTTGT TCGAATGGAA CAATGCAGCG AATTCGGGAG
    TGTAACGGGC CCTCGTATGG GGGCTTAGAG TGTCAAGGCG AGTGGCACGA GACACGGGAT
    TGCTTCCTTA GAGAATGTCC TGTGGATGGC CAATGGCAAC TTTGGAGCAC TTGGTCTGGT
    TGCACCAAAA CCTGTGGTGG AGGAAGTCAG CAGAGACAGA GGCTGTGTTA TGGTCCCTTT
    TTTGGGGGAG AGGCATGCCC GGGGGACCGA GAAGAAGTTA GACGTTGCAA TGAGAAGCGA
    TGCCCAGAGC CACATGAAAT CTGTGATGAG GAGAATTTCT CTAATGTAGT GTGGAAGAAA
    ACACCTGCAG GGGACACAGC GGCTGTCCGC TGCCCACCAA ATGCTGTAGG ATTAATTCTA
    CGGCGATGTT CATTAGACAA TGAGGGTATA GCATACTGGG AGAACCCAAC ATATATGAAG
    TGCGTCTCAA ATGATTACAG AAGTATCCAA ACACTGACCA GAGATCACCT GTCCAAGGCG
    CAGCGAGGGC TTGTGGGGGA TGGTGTTTCT GAAGTGATGA CAAAGCTGAG GGTGACGTCA
    AGTGATGGAA CGAGCTACAG CGGAGATCTT TTGGCTATCA TAGATGTGCT AAAAAACATG
    ACAGAGATAT TTAGGAGATC CTACTACAGC CCCAGTAATG CAGACATGAG GAATTTTGTG
    CAGTCTGTGA GTAACTTACT GATGGAGGAA AACAGGGACA GGTGGGAGGA AGCACAACTG
    CTGGGACCCA ATGTTAAGGA GCTTTTTCGT CTAGTAGAGG ACTTTGTGGA CGTCATCAGC
    CTTCGCATGA AGGACTTCCA GGATACTTAT GAGGTCACGG ATAATCTAGT GTTGAGCATT
    CACAAGCGGC CCGTGGCTGG AAATGCAGAT ATCACTTTCC CTATGAAGGG CTGGAGGGGG
    ATGGTGGACT GGGCTCGCAG CTCTGAGGAC AAAGTCAGAG TGGCCAAAAA CATCCTGCTC
    ACTGGAAAAC CGGAAGAAGA TGACGCTTCA GCTTTTGTGA CTGGGATCGT CCTGTACAGA
    AACTTAGGCA GTATTCTCTC TCTGCAGAGA AATAGCACAG TGCTGAACTC CAAGGTGATC
    TCTGTGACAA TCAAGCCCAC TCCCAGCTCT CTCTCGAGTC CACTAGAGAT TGAGTTCTCA
    CACCTTTACA ATGGAACCAC AAACCAAACC TGCATTTCCT GGGATGAGAA CGACAGCTCG
    TCGTTGCTGG GCTCCTGGTC TGCGCGAGGC TGTAGAGCCG TGCCCGTCGA CTCCAGCACA
    ACCAAATGCT TGTGTGACAG ACTCTCCACC TTCGCCATTT TAGCACGCCT CAATCCCGAT
    GTGAACATGG ATAAGACCCA GCTTCCATCT GTGACTCTGA TCGTGGGCTG CGGGGTCTCG
    TCCCTCACCC TGCTCCTCCT CATCATCATC TATGTTTCTG TTTGGAGGTA CATCCGCTCA
    GAGCGCTCTG TCATCCTCAT CAACTTCTGT CTGTCCATCA TCTCCTCCAA TGCGCTTATA
    CTCATCGGTC AAACTCAGAC ACGGAACAAG GTTGTTTGTA CTCTCGTGGC TGCGTTTCTG
    CACTTTTTCT TCCTGTCCTC TTTCTGTTGG GTTCTAACGG AGGCATGGCA GTCGTACATG
    GCTGTGACCG GCCGCCTTAG AAACCGTATC ATACGCAAGC GTTTCCTTTG CCTTGGTTGG
    GGTTTACCTG CTCTCGTTGT AGCAATTTCT GTTGGATTCA CCAAGGCAAA GGGATATGGC
    ACTGTTAACT ATTGCTGGCT GTCTCTGGAA GGAGGGCTGT TGTACGCATT TGTGGGACCA
    GCTGCTGCTG TCGTCCTGGT TAACATGGTT ATCGGGATTC TGGTTTTTAA CAAACTGGTG
    TCCAAAGATG GCATTACTGA TATGAAACTC AAGGAGAGGG CCGGTCAGAT GACAGTGCCA
    CTGTACAATA TGACGCTCAA ATGTGCCAAG TGCGGAGTTA TCTCGTCGCC TGACGTTTCC
    ACCACAGCTA CCAGTAACGC CATGGCGTCG CTGTGGAGCT CCTGCGTTGT TCTGCCGTTG
    CTGGCGCTCA CTTGGATGTC GGCAGTCTTG GCTATTACTG ACCGCCGTTC AGCCTTGTTT
    CAAATCCTTT TTGCCGTCTT TGACTCCCTC GAGGGCTTTG TTATCGTGAT GGTGCATTGC
    ATCCTACGCA GAGAGGTTCA AGAAGCAGTA AAGTGTCGTG TTGTTGACCG TCAAGAAGAT
    GGAAACGGTG ACTCTGGTGG ATCCTTCCAG AACGGTCATG CTCAGCTGAT GACTGACTTT
    GAAAAGGATG TGGACATAGC TTGTCGATCA GGCACCCTGA AGCGTCCCTC CCAGCAAGGC
    GAGGAGAAGG TTCCATCCCA GCAGATCACA GTACAGAAAG GCTCCAACTT CAACACCCTT
    CCTGCCAGCA TGGCAAAGGT CCACCTGCAA AACGTAGCCA ACTACGCCAG TCACACTCTC
    ACCATGCGCC GCGACAAAGG CGCCATCGCC GGGGTCAGCA CGGAGTTACC CGGGGCCAAA
    TCTATTTACA TCTGTGACGG CGAGCTCTTC AAACAGCTTG ACGGAGCACC GGGAGACATT
    CCTGAAGGAG GTGGTGGAGG TGCAGGTCAG GGTTATGTGC TTTTGCCCAA CAACAACAAC
    ACCCTTAGGC CTACGAAGGG TGGCAAGGAA GACCCGGCCG CCAAATATAA CATCAGTATC
    GAACAGTTAC CCCAGACAAG GCTGATCCAC CTGGCCAATC CTGTTGGCAC CGATCCCGTG
    GCAGGCTTCG GGCTGAAGAG CTTGCCCACC GACCGAGTCA GCGTTTCATG CTCGGAGAGG
    GAGTCCCCGG TGCAGACGGT GCAGAATATC TCCAGCGAGT CCCAGATGAC AAATACGTGT
    GAGCAAGGGG ATTCTGGGAA CTCTGGGATG TCTAAGAGCG AGACTGTCTC CACACTGTCC
    ATGAGCTCTC TGGAGAGACG AAAATCACGC TATGCAGAGC TGGACTTTGA GAAGATCATG
    CACACACGGA AGCGTCATCA GGACATGTTT CAGGACCTGA ATCGCAAACT GCAGCACGCA
    GAGAAGGACA GAGACTCTCC TCCAGTCGAC TGCAAGCCTG GAAAGAGATG GAGCATTTCG
    TCAGGAGGAA GCGACAAAAC CAACCTTAGT GACAAGCAGC AGACCCCCAG TAAGCGGGCA
    TGGGAGGGCA TCAGGAAGAC TCACTCTCCT CCATCATGGG TGCGCAAGGA GCTGGAGCCG
    CTGCAGGCCT CACCGCTGGA GATGCAGGCA GTGGAGTGGG AGAAGGCCAG TGCCACCATC
    CCTCTAGTGG GCCAGGAGAT CATGGACCTG CAGACGGAGG TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44100
    Clone ID Related Accession (Same CDS sequence) XM_021466979.1
    Accession Version XM_021466979.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4728bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product brain-specific angiogenesis inhibitor 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCT ACAGGCAGTA TTCCTCAAAA
    GGCCAGGGTT TAAGTCCAGT GAAATACAAA GGAATGCAAG AGGCCATGCT TGTGGATTCT
    CCGTCGGGTG AGGAGTGGTC TGCGTGGAGC GTGTGTTCGG CCACGTGTGG TGAGGGCTGG
    CAGAGCCGCA CACGCTTCTG CGTCTCCGTG TCGTACAGCA CGCAGTGCAG CGGGCCACTG
    CGCGAACAGA GACCCTGCAA TAACACTGCT GTGTGTCCAG CCGCTCTGAT TACAGAACAT
    GGGGCGTGGG GCGAGTGGAC ACCATGGAGC TTGTGTTCAT CAACATGCGG CCGTGGCTAC
    AGGGAACGCA ACCGCTCCTG CAAGCCACCC CAGTTCGGAG GAAACCCCTG TGATGGTCCA
    GAGAAACAGA CTAAATTCTG CAACATCGCT GTATGCCCAG TGGATGGCGT GTGGAACGAG
    TGGTCTGGTT GGAGTTCCTG TTCTTCAACT TGTTCGAATG GAACAATGCA GCGAATTCGG
    GAGTGTAACG GGCCCTCGTA TGGGGGCTTA GAGTGTCAAG GCGAGTGGCA CGAGACACGG
    GATTGCTTCC TTAGAGAATG TCCTGTGGAT GGCCAATGGC AACTTTGGAG CACTTGGTCT
    GGTTGCACCA AAACCTGTGG TGGAGGAAGT CAGCAGAGAC AGAGGCTGTG TTATGGTCCC
    TTTTTTGGGG GAGAGGCATG CCCGGGGGAC CGAGAAGAAG TTAGACGTTG CAATGAGAAG
    CGATGCCCAG AGCCACATGA AATCTGTGAT GAGGAGAATT TCTCTAATGT AGTGTGGAAG
    AAAACACCTG CAGGGGACAC AGCGGCTGTC CGCTGCCCAC CAAATGCTGT AGGATTAATT
    CTACGGCGAT GTTCATTAGA CAATGAGGGT ATAGCATACT GGGAGAACCC AACATATATG
    AAGTGCGTCT CAAATGATTA CAGAAGTATC CAAACACTGA CCAGAGATCA CCTGTCCAAG
    GCGCAGCGAG GGCTTGTGGG GGATGGTGTT TCTGAAGTGA TGACAAAGCT GAGGGTGACG
    TCAAGTGATG GAACGAGCTA CAGCGGAGAT CTTTTGGCTA TCATAGATGT GCTAAAAAAC
    ATGACAGAGA TATTTAGGAG ATCCTACTAC AGCCCCAGTA ATGCAGACAT GAGGAATTTT
    GTGCAGTCTG TGAGTAACTT ACTGATGGAG GAAAACAGGG ACAGGTGGGA GGAAGCACAA
    CTGCTGGGAC CCAATGTTAA GGAGCTTTTT CGTCTAGTAG AGGACTTTGT GGACGTCATC
    AGCCTTCGCA TGAAGGACTT CCAGGATACT TATGAGGTCA CGGATAATCT AGTGTTGAGC
    ATTCACAAGC GGCCCGTGGC TGGAAATGCA GATATCACTT TCCCTATGAA GGGCTGGAGG
    GGGATGGTGG ACTGGGCTCG CAGCTCTGAG GACAAAGTCA GAGTGGCCAA AAACATCCTG
    CTCACTGGAA AACCGGAAGA AGATGACGCT TCAGCTTTTG TGACTGGGAT CGTCCTGTAC
    AGAAACTTAG GCAGTATTCT CTCTCTGCAG AGAAATAGCA CAGTGCTGAA CTCCAAGGTG
    ATCTCTGTGA CAATCAAGCC CACTCCCAGC TCTCTCTCGA GTCCACTAGA GATTGAGTTC
    TCACACCTTT ACAATGGAAC CACAAACCAA ACCTGCATTT CCTGGGATGA GAACGACAGC
    TCGTCGTTGC TGGGCTCCTG GTCTGCGCGA GGCTGTAGAG CCGTGCCCGT CGACTCCAGC
    ACAACCAAAT GCTTGTGTGA CAGACTCTCC ACCTTCGCCA TTTTAGCACG CCTCAATCCC
    GATGTGAACA TGGATAAGAC CCAGCTTCCA TCTGTGACTC TGATCGTGGG CTGCGGGGTC
    TCGTCCCTCA CCCTGCTCCT CCTCATCATC ATCTATGTTT CTGTTTGGAG GTACATCCGC
    TCAGAGCGCT CTGTCATCCT CATCAACTTC TGTCTGTCCA TCATCTCCTC CAATGCGCTT
    ATACTCATCG GTCAAACTCA GACACGGAAC AAGGTTGTTT GTACTCTCGT GGCTGCGTTT
    CTGCACTTTT TCTTCCTGTC CTCTTTCTGT TGGGTTCTAA CGGAGGCATG GCAGTCGTAC
    ATGGCTGTGA CCGGCCGCCT TAGAAACCGT ATCATACGCA AGCGTTTCCT TTGCCTTGGT
    TGGGGTTTAC CTGCTCTCGT TGTAGCAATT TCTGTTGGAT TCACCAAGGC AAAGGGATAT
    GGCACTGTTA ACTATTGCTG GCTGTCTCTG GAAGGAGGGC TGTTGTACGC ATTTGTGGGA
    CCAGCTGCTG CTGTCGTCCT GGTTAACATG GTTATCGGGA TTCTGGTTTT TAACAAACTG
    GTGTCCAAAG ATGGCATTAC TGATATGAAA CTCAAGGAGA GGGCCGGTCA GATGACAGTG
    CCACTGTACA ATATGACGCT CAAATGTGCC AAGTGCGGAG TTATCTCGTC GCCTGACGTT
    TCCACCACAG CTACCAGTAA CGCCATGGCG TCGCTGTGGA GCTCCTGCGT TGTTCTGCCG
    TTGCTGGCGC TCACTTGGAT GTCGGCAGTC TTGGCTATTA CTGACCGCCG TTCAGCCTTG
    TTTCAAATCC TTTTTGCCGT CTTTGACTCC CTCGAGGGCT TTGTTATCGT GATGGTGCAT
    TGCATCCTAC GCAGAGAGGT TCAAGAAGCA GTAAAGTGTC GTGTTGTTGA CCGTCAAGAA
    GATGGAAACG GTGACTCTGG TGGATCCTTC CAGAACGGTC ATGCTCAGCT GATGACTGAC
    TTTGAAAAGG ATGTGGACAT AGCTTGTCGA TCAGGCACCC TGAAGCGTCC CTCCCAGCAA
    GGCGAGGAGA AGGTTCCATC CCAGCAGATC ACAGTACAGA AAGGCTCCAA CTTCAACACC
    CTTCCTGCCA GCATGGCAAA GGTCCACCTG CAAAACGTAG CCAACTACGC CAGTCACACT
    CTCACCATGC GCCGCGACAA AGGCGCCATC GCCGGGGTCA GCACGGAGTT ACCCGGGGCC
    AAATCTATTT ACATCTGTGA CGGCGAGCTC TTCAAACAGC TTGACGGAGC ACCGGGAGAC
    ATTCCTGAAG GAGGTGGTGG AGGTGCAGGT CAGGGTTATG TGCTTTTGCC CAACAACAAC
    AACACCCTTA GGCCTACGAA GGGTGGCAAG GAAGACCCGG CCGCCAAATA TAACATCAGT
    ATCGAACAGT TACCCCAGAC AAGGCTGATC CACCTGGCCA ATCCTGTTGG CACCGATCCC
    GTGGCAGGCT TCGGGCTGAA GAGCTTGCCC ACCGACCGAG TCAGCGTTTC ATGCTCGGAG
    AGGGAGTCCC CGGTGCAGAC GGTGCAGAAT ATCTCCAGCG AGTCCCAGAT GACAAATACG
    TGTGAGCAAG GGGATTCTGG GAACTCTGGG ATGTCTAAGA GCGAGACTGT CTCCACACTG
    TCCATGAGCT CTCTGGAGAG ACGAAAATCA CGCTATGCAG AGCTGGACTT TGAGAAGATC
    ATGCACACAC GGAAGCGTCA TCAGGACATG TTTCAGGACC TGAATCGCAA ACTGCAGCAC
    GCAGAGAAGG ACAGAGACTC TCCTCCAGTC GACTGCAAGC CTGGAAAGAG ATGGAGCATT
    TCGTCAGGAG GAAGCGACAA AACCAACCTT AGTGACAAGC AGCAGACCCC CAGTAAGCGG
    GCATGGGAGG GCATCAGGAA GACTCACTCT CCTCCATCAT GGGTGCGCAA GGAGCTGGAG
    CCGCTGCAGG CCTCACCGCT GGAGATGCAG GCAGTGGAGT GGGAGAAGGC CAGTGCCACC
    ATCCCTCTAG TGGGCCAGGA GATCATGGAC CTGCAGACGG AGGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322654.1
    CDS747..5474
    Translation

    Target ORF information:

    RefSeq Version XM_021466979.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466979.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCT ACAGGCAGTA TTCCTCAAAA
    GGCCAGGGTT TAAGTCCAGT GAAATACAAA GGAATGCAAG AGGCCATGCT TGTGGATTCT
    CCGTCGGGTG AGGAGTGGTC TGCGTGGAGC GTGTGTTCGG CCACGTGTGG TGAGGGCTGG
    CAGAGCCGCA CACGCTTCTG CGTCTCCGTG TCGTACAGCA CGCAGTGCAG CGGGCCACTG
    CGCGAACAGA GACCCTGCAA TAACACTGCT GTGTGTCCAG CCGCTCTGAT TACAGAACAT
    GGGGCGTGGG GCGAGTGGAC ACCATGGAGC TTGTGTTCAT CAACATGCGG CCGTGGCTAC
    AGGGAACGCA ACCGCTCCTG CAAGCCACCC CAGTTCGGAG GAAACCCCTG TGATGGTCCA
    GAGAAACAGA CTAAATTCTG CAACATCGCT GTATGCCCAG TGGATGGCGT GTGGAACGAG
    TGGTCTGGTT GGAGTTCCTG TTCTTCAACT TGTTCGAATG GAACAATGCA GCGAATTCGG
    GAGTGTAACG GGCCCTCGTA TGGGGGCTTA GAGTGTCAAG GCGAGTGGCA CGAGACACGG
    GATTGCTTCC TTAGAGAATG TCCTGTGGAT GGCCAATGGC AACTTTGGAG CACTTGGTCT
    GGTTGCACCA AAACCTGTGG TGGAGGAAGT CAGCAGAGAC AGAGGCTGTG TTATGGTCCC
    TTTTTTGGGG GAGAGGCATG CCCGGGGGAC CGAGAAGAAG TTAGACGTTG CAATGAGAAG
    CGATGCCCAG AGCCACATGA AATCTGTGAT GAGGAGAATT TCTCTAATGT AGTGTGGAAG
    AAAACACCTG CAGGGGACAC AGCGGCTGTC CGCTGCCCAC CAAATGCTGT AGGATTAATT
    CTACGGCGAT GTTCATTAGA CAATGAGGGT ATAGCATACT GGGAGAACCC AACATATATG
    AAGTGCGTCT CAAATGATTA CAGAAGTATC CAAACACTGA CCAGAGATCA CCTGTCCAAG
    GCGCAGCGAG GGCTTGTGGG GGATGGTGTT TCTGAAGTGA TGACAAAGCT GAGGGTGACG
    TCAAGTGATG GAACGAGCTA CAGCGGAGAT CTTTTGGCTA TCATAGATGT GCTAAAAAAC
    ATGACAGAGA TATTTAGGAG ATCCTACTAC AGCCCCAGTA ATGCAGACAT GAGGAATTTT
    GTGCAGTCTG TGAGTAACTT ACTGATGGAG GAAAACAGGG ACAGGTGGGA GGAAGCACAA
    CTGCTGGGAC CCAATGTTAA GGAGCTTTTT CGTCTAGTAG AGGACTTTGT GGACGTCATC
    AGCCTTCGCA TGAAGGACTT CCAGGATACT TATGAGGTCA CGGATAATCT AGTGTTGAGC
    ATTCACAAGC GGCCCGTGGC TGGAAATGCA GATATCACTT TCCCTATGAA GGGCTGGAGG
    GGGATGGTGG ACTGGGCTCG CAGCTCTGAG GACAAAGTCA GAGTGGCCAA AAACATCCTG
    CTCACTGGAA AACCGGAAGA AGATGACGCT TCAGCTTTTG TGACTGGGAT CGTCCTGTAC
    AGAAACTTAG GCAGTATTCT CTCTCTGCAG AGAAATAGCA CAGTGCTGAA CTCCAAGGTG
    ATCTCTGTGA CAATCAAGCC CACTCCCAGC TCTCTCTCGA GTCCACTAGA GATTGAGTTC
    TCACACCTTT ACAATGGAAC CACAAACCAA ACCTGCATTT CCTGGGATGA GAACGACAGC
    TCGTCGTTGC TGGGCTCCTG GTCTGCGCGA GGCTGTAGAG CCGTGCCCGT CGACTCCAGC
    ACAACCAAAT GCTTGTGTGA CAGACTCTCC ACCTTCGCCA TTTTAGCACG CCTCAATCCC
    GATGTGAACA TGGATAAGAC CCAGCTTCCA TCTGTGACTC TGATCGTGGG CTGCGGGGTC
    TCGTCCCTCA CCCTGCTCCT CCTCATCATC ATCTATGTTT CTGTTTGGAG GTACATCCGC
    TCAGAGCGCT CTGTCATCCT CATCAACTTC TGTCTGTCCA TCATCTCCTC CAATGCGCTT
    ATACTCATCG GTCAAACTCA GACACGGAAC AAGGTTGTTT GTACTCTCGT GGCTGCGTTT
    CTGCACTTTT TCTTCCTGTC CTCTTTCTGT TGGGTTCTAA CGGAGGCATG GCAGTCGTAC
    ATGGCTGTGA CCGGCCGCCT TAGAAACCGT ATCATACGCA AGCGTTTCCT TTGCCTTGGT
    TGGGGTTTAC CTGCTCTCGT TGTAGCAATT TCTGTTGGAT TCACCAAGGC AAAGGGATAT
    GGCACTGTTA ACTATTGCTG GCTGTCTCTG GAAGGAGGGC TGTTGTACGC ATTTGTGGGA
    CCAGCTGCTG CTGTCGTCCT GGTTAACATG GTTATCGGGA TTCTGGTTTT TAACAAACTG
    GTGTCCAAAG ATGGCATTAC TGATATGAAA CTCAAGGAGA GGGCCGGTCA GATGACAGTG
    CCACTGTACA ATATGACGCT CAAATGTGCC AAGTGCGGAG TTATCTCGTC GCCTGACGTT
    TCCACCACAG CTACCAGTAA CGCCATGGCG TCGCTGTGGA GCTCCTGCGT TGTTCTGCCG
    TTGCTGGCGC TCACTTGGAT GTCGGCAGTC TTGGCTATTA CTGACCGCCG TTCAGCCTTG
    TTTCAAATCC TTTTTGCCGT CTTTGACTCC CTCGAGGGCT TTGTTATCGT GATGGTGCAT
    TGCATCCTAC GCAGAGAGGT TCAAGAAGCA GTAAAGTGTC GTGTTGTTGA CCGTCAAGAA
    GATGGAAACG GTGACTCTGG TGGATCCTTC CAGAACGGTC ATGCTCAGCT GATGACTGAC
    TTTGAAAAGG ATGTGGACAT AGCTTGTCGA TCAGGCACCC TGAAGCGTCC CTCCCAGCAA
    GGCGAGGAGA AGGTTCCATC CCAGCAGATC ACAGTACAGA AAGGCTCCAA CTTCAACACC
    CTTCCTGCCA GCATGGCAAA GGTCCACCTG CAAAACGTAG CCAACTACGC CAGTCACACT
    CTCACCATGC GCCGCGACAA AGGCGCCATC GCCGGGGTCA GCACGGAGTT ACCCGGGGCC
    AAATCTATTT ACATCTGTGA CGGCGAGCTC TTCAAACAGC TTGACGGAGC ACCGGGAGAC
    ATTCCTGAAG GAGGTGGTGG AGGTGCAGGT CAGGGTTATG TGCTTTTGCC CAACAACAAC
    AACACCCTTA GGCCTACGAA GGGTGGCAAG GAAGACCCGG CCGCCAAATA TAACATCAGT
    ATCGAACAGT TACCCCAGAC AAGGCTGATC CACCTGGCCA ATCCTGTTGG CACCGATCCC
    GTGGCAGGCT TCGGGCTGAA GAGCTTGCCC ACCGACCGAG TCAGCGTTTC ATGCTCGGAG
    AGGGAGTCCC CGGTGCAGAC GGTGCAGAAT ATCTCCAGCG AGTCCCAGAT GACAAATACG
    TGTGAGCAAG GGGATTCTGG GAACTCTGGG ATGTCTAAGA GCGAGACTGT CTCCACACTG
    TCCATGAGCT CTCTGGAGAG ACGAAAATCA CGCTATGCAG AGCTGGACTT TGAGAAGATC
    ATGCACACAC GGAAGCGTCA TCAGGACATG TTTCAGGACC TGAATCGCAA ACTGCAGCAC
    GCAGAGAAGG ACAGAGACTC TCCTCCAGTC GACTGCAAGC CTGGAAAGAG ATGGAGCATT
    TCGTCAGGAG GAAGCGACAA AACCAACCTT AGTGACAAGC AGCAGACCCC CAGTAAGCGG
    GCATGGGAGG GCATCAGGAA GACTCACTCT CCTCCATCAT GGGTGCGCAA GGAGCTGGAG
    CCGCTGCAGG CCTCACCGCT GGAGATGCAG GCAGTGGAGT GGGAGAAGGC CAGTGCCACC
    ATCCCTCTAG TGGGCCAGGA GATCATGGAC CTGCAGACGG AGGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44094
    Clone ID Related Accession (Same CDS sequence) XM_009292551.2 , XM_009292551.3
    Accession Version XM_009292551.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4833bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product brain-specific angiogenesis inhibitor 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292551.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCT ACAGGCAGTA TTCCTCAAAA
    GGCCAGGGTT TAAGTCCAGT GAAATACAAA GGAATGCAAG AGGCCATGCT TGGCAAAGGC
    CGGCTTGGCT CTCGTAGCCA ATCCCTGCGC TCGGTGGACA GCCGTAAACG AGAGGACTCT
    GATAAGACCC GCAGCGGACA GCAGTCTTCC CAAACAGTGG ATTCTCCGTC GGGTGAGGAG
    TGGTCTGCGT GGAGCGTGTG TTCGGCCACG TGTGGTGAGG GCTGGCAGAG CCGCACACGC
    TTCTGCGTCT CCGTGTCGTA CAGCACGCAG TGCAGCGGGC CACTGCGCGA ACAGAGACCC
    TGCAATAACA CTGCTGTGTG TCCAGCCGCT CTGATTACAG AACATGGGGC GTGGGGCGAG
    TGGACACCAT GGAGCTTGTG TTCATCAACA TGCGGCCGTG GCTACAGGGA ACGCAACCGC
    TCCTGCAAGC CACCCCAGTT CGGAGGAAAC CCCTGTGATG GTCCAGAGAA ACAGACTAAA
    TTCTGCAACA TCGCTGTATG CCCAGTGGAT GGCGTGTGGA ACGAGTGGTC TGGTTGGAGT
    TCCTGTTCTT CAACTTGTTC GAATGGAACA ATGCAGCGAA TTCGGGAGTG TAACGGGCCC
    TCGTATGGGG GCTTAGAGTG TCAAGGCGAG TGGCACGAGA CACGGGATTG CTTCCTTAGA
    GAATGTCCTG TGGATGGCCA ATGGCAACTT TGGAGCACTT GGTCTGGTTG CACCAAAACC
    TGTGGTGGAG GAAGTCAGCA GAGACAGAGG CTGTGTTATG GTCCCTTTTT TGGGGGAGAG
    GCATGCCCGG GGGACCGAGA AGAAGTTAGA CGTTGCAATG AGAAGCGATG CCCAGAGCCA
    CATGAAATCT GTGATGAGGA GAATTTCTCT AATGTAGTGT GGAAGAAAAC ACCTGCAGGG
    GACACAGCGG CTGTCCGCTG CCCACCAAAT GCTGTAGGAT TAATTCTACG GCGATGTTCA
    TTAGACAATG AGGGTATAGC ATACTGGGAG AACCCAACAT ATATGAAGTG CGTCTCAAAT
    GATTACAGAA GTATCCAAAC ACTGACCAGA GATCACCTGT CCAAGGCGCA GCGAGGGCTT
    GTGGGGGATG GTGTTTCTGA AGTGATGACA AAGCTGAGGG TGACGTCAAG TGATGGAACG
    AGCTACAGCG GAGATCTTTT GGCTATCATA GATGTGCTAA AAAACATGAC AGAGATATTT
    AGGAGATCCT ACTACAGCCC CAGTAATGCA GACATGAGGA ATTTTGTGCA GTCTGTGAGT
    AACTTACTGA TGGAGGAAAA CAGGGACAGG TGGGAGGAAG CACAACTGCT GGGACCCAAT
    GTTAAGGAGC TTTTTCGTCT AGTAGAGGAC TTTGTGGACG TCATCAGCCT TCGCATGAAG
    GACTTCCAGG ATACTTATGA GGTCACGGAT AATCTAGTGT TGAGCATTCA CAAGCGGCCC
    GTGGCTGGAA ATGCAGATAT CACTTTCCCT ATGAAGGGCT GGAGGGGGAT GGTGGACTGG
    GCTCGCAGCT CTGAGGACAA AGTCAGAGTG GCCAAAAACA TCCTGCTCAC TGGAAAACCG
    GAAGAAGATG ACGCTTCAGC TTTTGTGACT GGGATCGTCC TGTACAGAAA CTTAGGCAGT
    ATTCTCTCTC TGCAGAGAAA TAGCACAGTG CTGAACTCCA AGGTGATCTC TGTGACAATC
    AAGCCCACTC CCAGCTCTCT CTCGAGTCCA CTAGAGATTG AGTTCTCACA CCTTTACAAT
    GGAACCACAA ACCAAACCTG CATTTCCTGG GATGAGAACG ACAGCTCGTC GTTGCTGGGC
    TCCTGGTCTG CGCGAGGCTG TAGAGCCGTG CCCGTCGACT CCAGCACAAC CAAATGCTTG
    TGTGACAGAC TCTCCACCTT CGCCATTTTA GCACGCCTCA ATCCCGATGT GAACATGGAT
    AAGACCCAGC TTCCATCTGT GACTCTGATC GTGGGCTGCG GGGTCTCGTC CCTCACCCTG
    CTCCTCCTCA TCATCATCTA TGTTTCTGTT TGGAGGTACA TCCGCTCAGA GCGCTCTGTC
    ATCCTCATCA ACTTCTGTCT GTCCATCATC TCCTCCAATG CGCTTATACT CATCGGTCAA
    ACTCAGACAC GGAACAAGGT TGTTTGTACT CTCGTGGCTG CGTTTCTGCA CTTTTTCTTC
    CTGTCCTCTT TCTGTTGGGT TCTAACGGAG GCATGGCAGT CGTACATGGC TGTGACCGGC
    CGCCTTAGAA ACCGTATCAT ACGCAAGCGT TTCCTTTGCC TTGGTTGGGG TTTACCTGCT
    CTCGTTGTAG CAATTTCTGT TGGATTCACC AAGGCAAAGG GATATGGCAC TGTTAACTAT
    TGCTGGCTGT CTCTGGAAGG AGGGCTGTTG TACGCATTTG TGGGACCAGC TGCTGCTGTC
    GTCCTGGTTA ACATGGTTAT CGGGATTCTG GTTTTTAACA AACTGGTGTC CAAAGATGGC
    ATTACTGATA TGAAACTCAA GGAGAGGGCC GGTCAGATGA CAGTGCCACT GTACAATATG
    ACGCTCAAAT GTGCCAAGTG CGGAGTTATC TCGTCGCCTG ACGTTTCCAC CACAGCTACC
    AGTAACGCCA TGGCGTCGCT GTGGAGCTCC TGCGTTGTTC TGCCGTTGCT GGCGCTCACT
    TGGATGTCGG CAGTCTTGGC TATTACTGAC CGCCGTTCAG CCTTGTTTCA AATCCTTTTT
    GCCGTCTTTG ACTCCCTCGA GGGCTTTGTT ATCGTGATGG TGCATTGCAT CCTACGCAGA
    GAGGTTCAAG AAGCAGTAAA GTGTCGTGTT GTTGACCGTC AAGAAGATGG AAACGGTGAC
    TCTGGTGGAT CCTTCCAGAA CGGTCATGCT CAGCTGATGA CTGACTTTGA AAAGGATGTG
    GACATAGCTT GTCGATCAGG CACCCTGAAG CGTCCCTCCC AGCAAGGCGA GGAGAAGGTT
    CCATCCCAGC AGATCACAGT ACAGAAAGGC TCCAACTTCA ACACCCTTCC TGCCAGCATG
    GCAAAGGTCC ACCTGCAAAA CGTAGCCAAC TACGCCAGTC ACACTCTCAC CATGCGCCGC
    GACAAAGGCG CCATCGCCGG GGTCAGCACG GAGTTACCCG GGGCCAAATC TATTTACATC
    TGTGACGGCG AGCTCTTCAA ACAGCTTGAC GGAGCACCGG GAGACATTCC TGAAGGAGGT
    GGTGGAGGTG CAGGTCAGGG TTATGTGCTT TTGCCCAACA ACAACAACAC CCTTAGGCCT
    ACGAAGGGTG GCAAGGAAGA CCCGGCCGCC AAATATAACA TCAGTATCGA ACAGTTACCC
    CAGACAAGGC TGATCCACCT GGCCAATCCT GTTGGCACCG ATCCCGTGGC AGGCTTCGGG
    CTGAAGAGCT TGCCCACCGA CCGAGTCAGC GTTTCATGCT CGGAGAGGGA GTCCCCGGTG
    CAGACGGTGC AGAATATCTC CAGCGAGTCC CAGATGACAA ATACGTGTGA GCAAGGGGAT
    TCTGGGAACT CTGGGATGTC TAAGAGCGAG ACTGTCTCCA CACTGTCCAT GAGCTCTCTG
    GAGAGACGAA AATCACGCTA TGCAGAGCTG GACTTTGAGA AGATCATGCA CACACGGAAG
    CGTCATCAGG ACATGTTTCA GGACCTGAAT CGCAAACTGC AGCACGCAGA GAAGGACAGA
    GACTCTCCTC CAGTCGACTG CAAGCCTGGA AAGAGATGGA GCATTTCGTC AGGAGGAAGC
    GACAAAACCA ACCTTAGTGA CAAGCAGCAG ACCCCCAGTA AGCGGGCATG GGAGGGCATC
    AGGAAGACTC ACTCTCCTCC ATCATGGGTG CGCAAGGAGC TGGAGCCGCT GCAGGCCTCA
    CCGCTGGAGA TGCAGGCAGT GGAGTGGGAG AAGGCCAGTG CCACCATCCC TCTAGTGGGC
    CAGGAGATCA TGGACCTGCA GACGGAGGTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290826.1
    CDS731..5563
    Translation

    Target ORF information:

    RefSeq Version XM_009292551.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292551.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCT ACAGGCAGTA TTCCTCAAAA
    GGCCAGGGTT TAAGTCCAGT GAAATACAAA GGAATGCAAG AGGCCATGCT TGGCAAAGGC
    CGGCTTGGCT CTCGTAGCCA ATCCCTGCGC TCGGTGGACA GCCGTAAACG AGAGGACTCT
    GATAAGACCC GCAGCGGACA GCAGTCTTCC CAAACAGTGG ATTCTCCGTC GGGTGAGGAG
    TGGTCTGCGT GGAGCGTGTG TTCGGCCACG TGTGGTGAGG GCTGGCAGAG CCGCACACGC
    TTCTGCGTCT CCGTGTCGTA CAGCACGCAG TGCAGCGGGC CACTGCGCGA ACAGAGACCC
    TGCAATAACA CTGCTGTGTG TCCAGCCGCT CTGATTACAG AACATGGGGC GTGGGGCGAG
    TGGACACCAT GGAGCTTGTG TTCATCAACA TGCGGCCGTG GCTACAGGGA ACGCAACCGC
    TCCTGCAAGC CACCCCAGTT CGGAGGAAAC CCCTGTGATG GTCCAGAGAA ACAGACTAAA
    TTCTGCAACA TCGCTGTATG CCCAGTGGAT GGCGTGTGGA ACGAGTGGTC TGGTTGGAGT
    TCCTGTTCTT CAACTTGTTC GAATGGAACA ATGCAGCGAA TTCGGGAGTG TAACGGGCCC
    TCGTATGGGG GCTTAGAGTG TCAAGGCGAG TGGCACGAGA CACGGGATTG CTTCCTTAGA
    GAATGTCCTG TGGATGGCCA ATGGCAACTT TGGAGCACTT GGTCTGGTTG CACCAAAACC
    TGTGGTGGAG GAAGTCAGCA GAGACAGAGG CTGTGTTATG GTCCCTTTTT TGGGGGAGAG
    GCATGCCCGG GGGACCGAGA AGAAGTTAGA CGTTGCAATG AGAAGCGATG CCCAGAGCCA
    CATGAAATCT GTGATGAGGA GAATTTCTCT AATGTAGTGT GGAAGAAAAC ACCTGCAGGG
    GACACAGCGG CTGTCCGCTG CCCACCAAAT GCTGTAGGAT TAATTCTACG GCGATGTTCA
    TTAGACAATG AGGGTATAGC ATACTGGGAG AACCCAACAT ATATGAAGTG CGTCTCAAAT
    GATTACAGAA GTATCCAAAC ACTGACCAGA GATCACCTGT CCAAGGCGCA GCGAGGGCTT
    GTGGGGGATG GTGTTTCTGA AGTGATGACA AAGCTGAGGG TGACGTCAAG TGATGGAACG
    AGCTACAGCG GAGATCTTTT GGCTATCATA GATGTGCTAA AAAACATGAC AGAGATATTT
    AGGAGATCCT ACTACAGCCC CAGTAATGCA GACATGAGGA ATTTTGTGCA GTCTGTGAGT
    AACTTACTGA TGGAGGAAAA CAGGGACAGG TGGGAGGAAG CACAACTGCT GGGACCCAAT
    GTTAAGGAGC TTTTTCGTCT AGTAGAGGAC TTTGTGGACG TCATCAGCCT TCGCATGAAG
    GACTTCCAGG ATACTTATGA GGTCACGGAT AATCTAGTGT TGAGCATTCA CAAGCGGCCC
    GTGGCTGGAA ATGCAGATAT CACTTTCCCT ATGAAGGGCT GGAGGGGGAT GGTGGACTGG
    GCTCGCAGCT CTGAGGACAA AGTCAGAGTG GCCAAAAACA TCCTGCTCAC TGGAAAACCG
    GAAGAAGATG ACGCTTCAGC TTTTGTGACT GGGATCGTCC TGTACAGAAA CTTAGGCAGT
    ATTCTCTCTC TGCAGAGAAA TAGCACAGTG CTGAACTCCA AGGTGATCTC TGTGACAATC
    AAGCCCACTC CCAGCTCTCT CTCGAGTCCA CTAGAGATTG AGTTCTCACA CCTTTACAAT
    GGAACCACAA ACCAAACCTG CATTTCCTGG GATGAGAACG ACAGCTCGTC GTTGCTGGGC
    TCCTGGTCTG CGCGAGGCTG TAGAGCCGTG CCCGTCGACT CCAGCACAAC CAAATGCTTG
    TGTGACAGAC TCTCCACCTT CGCCATTTTA GCACGCCTCA ATCCCGATGT GAACATGGAT
    AAGACCCAGC TTCCATCTGT GACTCTGATC GTGGGCTGCG GGGTCTCGTC CCTCACCCTG
    CTCCTCCTCA TCATCATCTA TGTTTCTGTT TGGAGGTACA TCCGCTCAGA GCGCTCTGTC
    ATCCTCATCA ACTTCTGTCT GTCCATCATC TCCTCCAATG CGCTTATACT CATCGGTCAA
    ACTCAGACAC GGAACAAGGT TGTTTGTACT CTCGTGGCTG CGTTTCTGCA CTTTTTCTTC
    CTGTCCTCTT TCTGTTGGGT TCTAACGGAG GCATGGCAGT CGTACATGGC TGTGACCGGC
    CGCCTTAGAA ACCGTATCAT ACGCAAGCGT TTCCTTTGCC TTGGTTGGGG TTTACCTGCT
    CTCGTTGTAG CAATTTCTGT TGGATTCACC AAGGCAAAGG GATATGGCAC TGTTAACTAT
    TGCTGGCTGT CTCTGGAAGG AGGGCTGTTG TACGCATTTG TGGGACCAGC TGCTGCTGTC
    GTCCTGGTTA ACATGGTTAT CGGGATTCTG GTTTTTAACA AACTGGTGTC CAAAGATGGC
    ATTACTGATA TGAAACTCAA GGAGAGGGCC GGTCAGATGA CAGTGCCACT GTACAATATG
    ACGCTCAAAT GTGCCAAGTG CGGAGTTATC TCGTCGCCTG ACGTTTCCAC CACAGCTACC
    AGTAACGCCA TGGCGTCGCT GTGGAGCTCC TGCGTTGTTC TGCCGTTGCT GGCGCTCACT
    TGGATGTCGG CAGTCTTGGC TATTACTGAC CGCCGTTCAG CCTTGTTTCA AATCCTTTTT
    GCCGTCTTTG ACTCCCTCGA GGGCTTTGTT ATCGTGATGG TGCATTGCAT CCTACGCAGA
    GAGGTTCAAG AAGCAGTAAA GTGTCGTGTT GTTGACCGTC AAGAAGATGG AAACGGTGAC
    TCTGGTGGAT CCTTCCAGAA CGGTCATGCT CAGCTGATGA CTGACTTTGA AAAGGATGTG
    GACATAGCTT GTCGATCAGG CACCCTGAAG CGTCCCTCCC AGCAAGGCGA GGAGAAGGTT
    CCATCCCAGC AGATCACAGT ACAGAAAGGC TCCAACTTCA ACACCCTTCC TGCCAGCATG
    GCAAAGGTCC ACCTGCAAAA CGTAGCCAAC TACGCCAGTC ACACTCTCAC CATGCGCCGC
    GACAAAGGCG CCATCGCCGG GGTCAGCACG GAGTTACCCG GGGCCAAATC TATTTACATC
    TGTGACGGCG AGCTCTTCAA ACAGCTTGAC GGAGCACCGG GAGACATTCC TGAAGGAGGT
    GGTGGAGGTG CAGGTCAGGG TTATGTGCTT TTGCCCAACA ACAACAACAC CCTTAGGCCT
    ACGAAGGGTG GCAAGGAAGA CCCGGCCGCC AAATATAACA TCAGTATCGA ACAGTTACCC
    CAGACAAGGC TGATCCACCT GGCCAATCCT GTTGGCACCG ATCCCGTGGC AGGCTTCGGG
    CTGAAGAGCT TGCCCACCGA CCGAGTCAGC GTTTCATGCT CGGAGAGGGA GTCCCCGGTG
    CAGACGGTGC AGAATATCTC CAGCGAGTCC CAGATGACAA ATACGTGTGA GCAAGGGGAT
    TCTGGGAACT CTGGGATGTC TAAGAGCGAG ACTGTCTCCA CACTGTCCAT GAGCTCTCTG
    GAGAGACGAA AATCACGCTA TGCAGAGCTG GACTTTGAGA AGATCATGCA CACACGGAAG
    CGTCATCAGG ACATGTTTCA GGACCTGAAT CGCAAACTGC AGCACGCAGA GAAGGACAGA
    GACTCTCCTC CAGTCGACTG CAAGCCTGGA AAGAGATGGA GCATTTCGTC AGGAGGAAGC
    GACAAAACCA ACCTTAGTGA CAAGCAGCAG ACCCCCAGTA AGCGGGCATG GGAGGGCATC
    AGGAAGACTC ACTCTCCTCC ATCATGGGTG CGCAAGGAGC TGGAGCCGCT GCAGGCCTCA
    CCGCTGGAGA TGCAGGCAGT GGAGTGGGAG AAGGCCAGTG CCACCATCCC TCTAGTGGGC
    CAGGAGATCA TGGACCTGCA GACGGAGGTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44094
    Clone ID Related Accession (Same CDS sequence) XM_009292551.2 , XM_009292551.3
    Accession Version XM_009292551.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4833bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product brain-specific angiogenesis inhibitor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009292551.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCT ACAGGCAGTA TTCCTCAAAA
    GGCCAGGGTT TAAGTCCAGT GAAATACAAA GGAATGCAAG AGGCCATGCT TGGCAAAGGC
    CGGCTTGGCT CTCGTAGCCA ATCCCTGCGC TCGGTGGACA GCCGTAAACG AGAGGACTCT
    GATAAGACCC GCAGCGGACA GCAGTCTTCC CAAACAGTGG ATTCTCCGTC GGGTGAGGAG
    TGGTCTGCGT GGAGCGTGTG TTCGGCCACG TGTGGTGAGG GCTGGCAGAG CCGCACACGC
    TTCTGCGTCT CCGTGTCGTA CAGCACGCAG TGCAGCGGGC CACTGCGCGA ACAGAGACCC
    TGCAATAACA CTGCTGTGTG TCCAGCCGCT CTGATTACAG AACATGGGGC GTGGGGCGAG
    TGGACACCAT GGAGCTTGTG TTCATCAACA TGCGGCCGTG GCTACAGGGA ACGCAACCGC
    TCCTGCAAGC CACCCCAGTT CGGAGGAAAC CCCTGTGATG GTCCAGAGAA ACAGACTAAA
    TTCTGCAACA TCGCTGTATG CCCAGTGGAT GGCGTGTGGA ACGAGTGGTC TGGTTGGAGT
    TCCTGTTCTT CAACTTGTTC GAATGGAACA ATGCAGCGAA TTCGGGAGTG TAACGGGCCC
    TCGTATGGGG GCTTAGAGTG TCAAGGCGAG TGGCACGAGA CACGGGATTG CTTCCTTAGA
    GAATGTCCTG TGGATGGCCA ATGGCAACTT TGGAGCACTT GGTCTGGTTG CACCAAAACC
    TGTGGTGGAG GAAGTCAGCA GAGACAGAGG CTGTGTTATG GTCCCTTTTT TGGGGGAGAG
    GCATGCCCGG GGGACCGAGA AGAAGTTAGA CGTTGCAATG AGAAGCGATG CCCAGAGCCA
    CATGAAATCT GTGATGAGGA GAATTTCTCT AATGTAGTGT GGAAGAAAAC ACCTGCAGGG
    GACACAGCGG CTGTCCGCTG CCCACCAAAT GCTGTAGGAT TAATTCTACG GCGATGTTCA
    TTAGACAATG AGGGTATAGC ATACTGGGAG AACCCAACAT ATATGAAGTG CGTCTCAAAT
    GATTACAGAA GTATCCAAAC ACTGACCAGA GATCACCTGT CCAAGGCGCA GCGAGGGCTT
    GTGGGGGATG GTGTTTCTGA AGTGATGACA AAGCTGAGGG TGACGTCAAG TGATGGAACG
    AGCTACAGCG GAGATCTTTT GGCTATCATA GATGTGCTAA AAAACATGAC AGAGATATTT
    AGGAGATCCT ACTACAGCCC CAGTAATGCA GACATGAGGA ATTTTGTGCA GTCTGTGAGT
    AACTTACTGA TGGAGGAAAA CAGGGACAGG TGGGAGGAAG CACAACTGCT GGGACCCAAT
    GTTAAGGAGC TTTTTCGTCT AGTAGAGGAC TTTGTGGACG TCATCAGCCT TCGCATGAAG
    GACTTCCAGG ATACTTATGA GGTCACGGAT AATCTAGTGT TGAGCATTCA CAAGCGGCCC
    GTGGCTGGAA ATGCAGATAT CACTTTCCCT ATGAAGGGCT GGAGGGGGAT GGTGGACTGG
    GCTCGCAGCT CTGAGGACAA AGTCAGAGTG GCCAAAAACA TCCTGCTCAC TGGAAAACCG
    GAAGAAGATG ACGCTTCAGC TTTTGTGACT GGGATCGTCC TGTACAGAAA CTTAGGCAGT
    ATTCTCTCTC TGCAGAGAAA TAGCACAGTG CTGAACTCCA AGGTGATCTC TGTGACAATC
    AAGCCCACTC CCAGCTCTCT CTCGAGTCCA CTAGAGATTG AGTTCTCACA CCTTTACAAT
    GGAACCACAA ACCAAACCTG CATTTCCTGG GATGAGAACG ACAGCTCGTC GTTGCTGGGC
    TCCTGGTCTG CGCGAGGCTG TAGAGCCGTG CCCGTCGACT CCAGCACAAC CAAATGCTTG
    TGTGACAGAC TCTCCACCTT CGCCATTTTA GCACGCCTCA ATCCCGATGT GAACATGGAT
    AAGACCCAGC TTCCATCTGT GACTCTGATC GTGGGCTGCG GGGTCTCGTC CCTCACCCTG
    CTCCTCCTCA TCATCATCTA TGTTTCTGTT TGGAGGTACA TCCGCTCAGA GCGCTCTGTC
    ATCCTCATCA ACTTCTGTCT GTCCATCATC TCCTCCAATG CGCTTATACT CATCGGTCAA
    ACTCAGACAC GGAACAAGGT TGTTTGTACT CTCGTGGCTG CGTTTCTGCA CTTTTTCTTC
    CTGTCCTCTT TCTGTTGGGT TCTAACGGAG GCATGGCAGT CGTACATGGC TGTGACCGGC
    CGCCTTAGAA ACCGTATCAT ACGCAAGCGT TTCCTTTGCC TTGGTTGGGG TTTACCTGCT
    CTCGTTGTAG CAATTTCTGT TGGATTCACC AAGGCAAAGG GATATGGCAC TGTTAACTAT
    TGCTGGCTGT CTCTGGAAGG AGGGCTGTTG TACGCATTTG TGGGACCAGC TGCTGCTGTC
    GTCCTGGTTA ACATGGTTAT CGGGATTCTG GTTTTTAACA AACTGGTGTC CAAAGATGGC
    ATTACTGATA TGAAACTCAA GGAGAGGGCC GGTCAGATGA CAGTGCCACT GTACAATATG
    ACGCTCAAAT GTGCCAAGTG CGGAGTTATC TCGTCGCCTG ACGTTTCCAC CACAGCTACC
    AGTAACGCCA TGGCGTCGCT GTGGAGCTCC TGCGTTGTTC TGCCGTTGCT GGCGCTCACT
    TGGATGTCGG CAGTCTTGGC TATTACTGAC CGCCGTTCAG CCTTGTTTCA AATCCTTTTT
    GCCGTCTTTG ACTCCCTCGA GGGCTTTGTT ATCGTGATGG TGCATTGCAT CCTACGCAGA
    GAGGTTCAAG AAGCAGTAAA GTGTCGTGTT GTTGACCGTC AAGAAGATGG AAACGGTGAC
    TCTGGTGGAT CCTTCCAGAA CGGTCATGCT CAGCTGATGA CTGACTTTGA AAAGGATGTG
    GACATAGCTT GTCGATCAGG CACCCTGAAG CGTCCCTCCC AGCAAGGCGA GGAGAAGGTT
    CCATCCCAGC AGATCACAGT ACAGAAAGGC TCCAACTTCA ACACCCTTCC TGCCAGCATG
    GCAAAGGTCC ACCTGCAAAA CGTAGCCAAC TACGCCAGTC ACACTCTCAC CATGCGCCGC
    GACAAAGGCG CCATCGCCGG GGTCAGCACG GAGTTACCCG GGGCCAAATC TATTTACATC
    TGTGACGGCG AGCTCTTCAA ACAGCTTGAC GGAGCACCGG GAGACATTCC TGAAGGAGGT
    GGTGGAGGTG CAGGTCAGGG TTATGTGCTT TTGCCCAACA ACAACAACAC CCTTAGGCCT
    ACGAAGGGTG GCAAGGAAGA CCCGGCCGCC AAATATAACA TCAGTATCGA ACAGTTACCC
    CAGACAAGGC TGATCCACCT GGCCAATCCT GTTGGCACCG ATCCCGTGGC AGGCTTCGGG
    CTGAAGAGCT TGCCCACCGA CCGAGTCAGC GTTTCATGCT CGGAGAGGGA GTCCCCGGTG
    CAGACGGTGC AGAATATCTC CAGCGAGTCC CAGATGACAA ATACGTGTGA GCAAGGGGAT
    TCTGGGAACT CTGGGATGTC TAAGAGCGAG ACTGTCTCCA CACTGTCCAT GAGCTCTCTG
    GAGAGACGAA AATCACGCTA TGCAGAGCTG GACTTTGAGA AGATCATGCA CACACGGAAG
    CGTCATCAGG ACATGTTTCA GGACCTGAAT CGCAAACTGC AGCACGCAGA GAAGGACAGA
    GACTCTCCTC CAGTCGACTG CAAGCCTGGA AAGAGATGGA GCATTTCGTC AGGAGGAAGC
    GACAAAACCA ACCTTAGTGA CAAGCAGCAG ACCCCCAGTA AGCGGGCATG GGAGGGCATC
    AGGAAGACTC ACTCTCCTCC ATCATGGGTG CGCAAGGAGC TGGAGCCGCT GCAGGCCTCA
    CCGCTGGAGA TGCAGGCAGT GGAGTGGGAG AAGGCCAGTG CCACCATCCC TCTAGTGGGC
    CAGGAGATCA TGGACCTGCA GACGGAGGTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290826.1
    CDS748..5580
    Translation

    Target ORF information:

    RefSeq Version XM_009292551.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292551.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCT ACAGGCAGTA TTCCTCAAAA
    GGCCAGGGTT TAAGTCCAGT GAAATACAAA GGAATGCAAG AGGCCATGCT TGGCAAAGGC
    CGGCTTGGCT CTCGTAGCCA ATCCCTGCGC TCGGTGGACA GCCGTAAACG AGAGGACTCT
    GATAAGACCC GCAGCGGACA GCAGTCTTCC CAAACAGTGG ATTCTCCGTC GGGTGAGGAG
    TGGTCTGCGT GGAGCGTGTG TTCGGCCACG TGTGGTGAGG GCTGGCAGAG CCGCACACGC
    TTCTGCGTCT CCGTGTCGTA CAGCACGCAG TGCAGCGGGC CACTGCGCGA ACAGAGACCC
    TGCAATAACA CTGCTGTGTG TCCAGCCGCT CTGATTACAG AACATGGGGC GTGGGGCGAG
    TGGACACCAT GGAGCTTGTG TTCATCAACA TGCGGCCGTG GCTACAGGGA ACGCAACCGC
    TCCTGCAAGC CACCCCAGTT CGGAGGAAAC CCCTGTGATG GTCCAGAGAA ACAGACTAAA
    TTCTGCAACA TCGCTGTATG CCCAGTGGAT GGCGTGTGGA ACGAGTGGTC TGGTTGGAGT
    TCCTGTTCTT CAACTTGTTC GAATGGAACA ATGCAGCGAA TTCGGGAGTG TAACGGGCCC
    TCGTATGGGG GCTTAGAGTG TCAAGGCGAG TGGCACGAGA CACGGGATTG CTTCCTTAGA
    GAATGTCCTG TGGATGGCCA ATGGCAACTT TGGAGCACTT GGTCTGGTTG CACCAAAACC
    TGTGGTGGAG GAAGTCAGCA GAGACAGAGG CTGTGTTATG GTCCCTTTTT TGGGGGAGAG
    GCATGCCCGG GGGACCGAGA AGAAGTTAGA CGTTGCAATG AGAAGCGATG CCCAGAGCCA
    CATGAAATCT GTGATGAGGA GAATTTCTCT AATGTAGTGT GGAAGAAAAC ACCTGCAGGG
    GACACAGCGG CTGTCCGCTG CCCACCAAAT GCTGTAGGAT TAATTCTACG GCGATGTTCA
    TTAGACAATG AGGGTATAGC ATACTGGGAG AACCCAACAT ATATGAAGTG CGTCTCAAAT
    GATTACAGAA GTATCCAAAC ACTGACCAGA GATCACCTGT CCAAGGCGCA GCGAGGGCTT
    GTGGGGGATG GTGTTTCTGA AGTGATGACA AAGCTGAGGG TGACGTCAAG TGATGGAACG
    AGCTACAGCG GAGATCTTTT GGCTATCATA GATGTGCTAA AAAACATGAC AGAGATATTT
    AGGAGATCCT ACTACAGCCC CAGTAATGCA GACATGAGGA ATTTTGTGCA GTCTGTGAGT
    AACTTACTGA TGGAGGAAAA CAGGGACAGG TGGGAGGAAG CACAACTGCT GGGACCCAAT
    GTTAAGGAGC TTTTTCGTCT AGTAGAGGAC TTTGTGGACG TCATCAGCCT TCGCATGAAG
    GACTTCCAGG ATACTTATGA GGTCACGGAT AATCTAGTGT TGAGCATTCA CAAGCGGCCC
    GTGGCTGGAA ATGCAGATAT CACTTTCCCT ATGAAGGGCT GGAGGGGGAT GGTGGACTGG
    GCTCGCAGCT CTGAGGACAA AGTCAGAGTG GCCAAAAACA TCCTGCTCAC TGGAAAACCG
    GAAGAAGATG ACGCTTCAGC TTTTGTGACT GGGATCGTCC TGTACAGAAA CTTAGGCAGT
    ATTCTCTCTC TGCAGAGAAA TAGCACAGTG CTGAACTCCA AGGTGATCTC TGTGACAATC
    AAGCCCACTC CCAGCTCTCT CTCGAGTCCA CTAGAGATTG AGTTCTCACA CCTTTACAAT
    GGAACCACAA ACCAAACCTG CATTTCCTGG GATGAGAACG ACAGCTCGTC GTTGCTGGGC
    TCCTGGTCTG CGCGAGGCTG TAGAGCCGTG CCCGTCGACT CCAGCACAAC CAAATGCTTG
    TGTGACAGAC TCTCCACCTT CGCCATTTTA GCACGCCTCA ATCCCGATGT GAACATGGAT
    AAGACCCAGC TTCCATCTGT GACTCTGATC GTGGGCTGCG GGGTCTCGTC CCTCACCCTG
    CTCCTCCTCA TCATCATCTA TGTTTCTGTT TGGAGGTACA TCCGCTCAGA GCGCTCTGTC
    ATCCTCATCA ACTTCTGTCT GTCCATCATC TCCTCCAATG CGCTTATACT CATCGGTCAA
    ACTCAGACAC GGAACAAGGT TGTTTGTACT CTCGTGGCTG CGTTTCTGCA CTTTTTCTTC
    CTGTCCTCTT TCTGTTGGGT TCTAACGGAG GCATGGCAGT CGTACATGGC TGTGACCGGC
    CGCCTTAGAA ACCGTATCAT ACGCAAGCGT TTCCTTTGCC TTGGTTGGGG TTTACCTGCT
    CTCGTTGTAG CAATTTCTGT TGGATTCACC AAGGCAAAGG GATATGGCAC TGTTAACTAT
    TGCTGGCTGT CTCTGGAAGG AGGGCTGTTG TACGCATTTG TGGGACCAGC TGCTGCTGTC
    GTCCTGGTTA ACATGGTTAT CGGGATTCTG GTTTTTAACA AACTGGTGTC CAAAGATGGC
    ATTACTGATA TGAAACTCAA GGAGAGGGCC GGTCAGATGA CAGTGCCACT GTACAATATG
    ACGCTCAAAT GTGCCAAGTG CGGAGTTATC TCGTCGCCTG ACGTTTCCAC CACAGCTACC
    AGTAACGCCA TGGCGTCGCT GTGGAGCTCC TGCGTTGTTC TGCCGTTGCT GGCGCTCACT
    TGGATGTCGG CAGTCTTGGC TATTACTGAC CGCCGTTCAG CCTTGTTTCA AATCCTTTTT
    GCCGTCTTTG ACTCCCTCGA GGGCTTTGTT ATCGTGATGG TGCATTGCAT CCTACGCAGA
    GAGGTTCAAG AAGCAGTAAA GTGTCGTGTT GTTGACCGTC AAGAAGATGG AAACGGTGAC
    TCTGGTGGAT CCTTCCAGAA CGGTCATGCT CAGCTGATGA CTGACTTTGA AAAGGATGTG
    GACATAGCTT GTCGATCAGG CACCCTGAAG CGTCCCTCCC AGCAAGGCGA GGAGAAGGTT
    CCATCCCAGC AGATCACAGT ACAGAAAGGC TCCAACTTCA ACACCCTTCC TGCCAGCATG
    GCAAAGGTCC ACCTGCAAAA CGTAGCCAAC TACGCCAGTC ACACTCTCAC CATGCGCCGC
    GACAAAGGCG CCATCGCCGG GGTCAGCACG GAGTTACCCG GGGCCAAATC TATTTACATC
    TGTGACGGCG AGCTCTTCAA ACAGCTTGAC GGAGCACCGG GAGACATTCC TGAAGGAGGT
    GGTGGAGGTG CAGGTCAGGG TTATGTGCTT TTGCCCAACA ACAACAACAC CCTTAGGCCT
    ACGAAGGGTG GCAAGGAAGA CCCGGCCGCC AAATATAACA TCAGTATCGA ACAGTTACCC
    CAGACAAGGC TGATCCACCT GGCCAATCCT GTTGGCACCG ATCCCGTGGC AGGCTTCGGG
    CTGAAGAGCT TGCCCACCGA CCGAGTCAGC GTTTCATGCT CGGAGAGGGA GTCCCCGGTG
    CAGACGGTGC AGAATATCTC CAGCGAGTCC CAGATGACAA ATACGTGTGA GCAAGGGGAT
    TCTGGGAACT CTGGGATGTC TAAGAGCGAG ACTGTCTCCA CACTGTCCAT GAGCTCTCTG
    GAGAGACGAA AATCACGCTA TGCAGAGCTG GACTTTGAGA AGATCATGCA CACACGGAAG
    CGTCATCAGG ACATGTTTCA GGACCTGAAT CGCAAACTGC AGCACGCAGA GAAGGACAGA
    GACTCTCCTC CAGTCGACTG CAAGCCTGGA AAGAGATGGA GCATTTCGTC AGGAGGAAGC
    GACAAAACCA ACCTTAGTGA CAAGCAGCAG ACCCCCAGTA AGCGGGCATG GGAGGGCATC
    AGGAAGACTC ACTCTCCTCC ATCATGGGTG CGCAAGGAGC TGGAGCCGCT GCAGGCCTCA
    CCGCTGGAGA TGCAGGCAGT GGAGTGGGAG AAGGCCAGTG CCACCATCCC TCTAGTGGGC
    CAGGAGATCA TGGACCTGCA GACGGAGGTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44095
    Clone ID Related Accession (Same CDS sequence) XM_009292552.3 , XM_009292552.2
    Accession Version XM_009292552.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4818bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product brain-specific angiogenesis inhibitor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009292552.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCT ACAGGCAGTA TTCCTCAAAA
    GGCCAGGGTT TAAGTCCAGT GAAATACAAA GGAATGCAAG AGGCCATGCT TGGCAAAGGC
    CGGCTTGGCT CTCGTAGCCA ATCCCTGCGC TCGGTGGACA GCCGTAAACG AGAGGACTCT
    GATAAGACCC GCAGCGGACA GCAGTCTTCC CAAACAGTGG ATTCTCCGTC GGGTGAGGAG
    TGGTCTGCGT GGAGCGTGTG TTCGGCCACG TGTGGTGAGG GCTGGCAGAG CCGCACACGC
    TTCTGCGTCT CCGTGTCGTA CAGCACGCAG TGCAGCGGGC CACTGCGCGA ACAGAGACCC
    TGCAATAACA CTGCTGTGTG TCCAGAACAT GGGGCGTGGG GCGAGTGGAC ACCATGGAGC
    TTGTGTTCAT CAACATGCGG CCGTGGCTAC AGGGAACGCA ACCGCTCCTG CAAGCCACCC
    CAGTTCGGAG GAAACCCCTG TGATGGTCCA GAGAAACAGA CTAAATTCTG CAACATCGCT
    GTATGCCCAG TGGATGGCGT GTGGAACGAG TGGTCTGGTT GGAGTTCCTG TTCTTCAACT
    TGTTCGAATG GAACAATGCA GCGAATTCGG GAGTGTAACG GGCCCTCGTA TGGGGGCTTA
    GAGTGTCAAG GCGAGTGGCA CGAGACACGG GATTGCTTCC TTAGAGAATG TCCTGTGGAT
    GGCCAATGGC AACTTTGGAG CACTTGGTCT GGTTGCACCA AAACCTGTGG TGGAGGAAGT
    CAGCAGAGAC AGAGGCTGTG TTATGGTCCC TTTTTTGGGG GAGAGGCATG CCCGGGGGAC
    CGAGAAGAAG TTAGACGTTG CAATGAGAAG CGATGCCCAG AGCCACATGA AATCTGTGAT
    GAGGAGAATT TCTCTAATGT AGTGTGGAAG AAAACACCTG CAGGGGACAC AGCGGCTGTC
    CGCTGCCCAC CAAATGCTGT AGGATTAATT CTACGGCGAT GTTCATTAGA CAATGAGGGT
    ATAGCATACT GGGAGAACCC AACATATATG AAGTGCGTCT CAAATGATTA CAGAAGTATC
    CAAACACTGA CCAGAGATCA CCTGTCCAAG GCGCAGCGAG GGCTTGTGGG GGATGGTGTT
    TCTGAAGTGA TGACAAAGCT GAGGGTGACG TCAAGTGATG GAACGAGCTA CAGCGGAGAT
    CTTTTGGCTA TCATAGATGT GCTAAAAAAC ATGACAGAGA TATTTAGGAG ATCCTACTAC
    AGCCCCAGTA ATGCAGACAT GAGGAATTTT GTGCAGTCTG TGAGTAACTT ACTGATGGAG
    GAAAACAGGG ACAGGTGGGA GGAAGCACAA CTGCTGGGAC CCAATGTTAA GGAGCTTTTT
    CGTCTAGTAG AGGACTTTGT GGACGTCATC AGCCTTCGCA TGAAGGACTT CCAGGATACT
    TATGAGGTCA CGGATAATCT AGTGTTGAGC ATTCACAAGC GGCCCGTGGC TGGAAATGCA
    GATATCACTT TCCCTATGAA GGGCTGGAGG GGGATGGTGG ACTGGGCTCG CAGCTCTGAG
    GACAAAGTCA GAGTGGCCAA AAACATCCTG CTCACTGGAA AACCGGAAGA AGATGACGCT
    TCAGCTTTTG TGACTGGGAT CGTCCTGTAC AGAAACTTAG GCAGTATTCT CTCTCTGCAG
    AGAAATAGCA CAGTGCTGAA CTCCAAGGTG ATCTCTGTGA CAATCAAGCC CACTCCCAGC
    TCTCTCTCGA GTCCACTAGA GATTGAGTTC TCACACCTTT ACAATGGAAC CACAAACCAA
    ACCTGCATTT CCTGGGATGA GAACGACAGC TCGTCGTTGC TGGGCTCCTG GTCTGCGCGA
    GGCTGTAGAG CCGTGCCCGT CGACTCCAGC ACAACCAAAT GCTTGTGTGA CAGACTCTCC
    ACCTTCGCCA TTTTAGCACG CCTCAATCCC GATGTGAACA TGGATAAGAC CCAGCTTCCA
    TCTGTGACTC TGATCGTGGG CTGCGGGGTC TCGTCCCTCA CCCTGCTCCT CCTCATCATC
    ATCTATGTTT CTGTTTGGAG GTACATCCGC TCAGAGCGCT CTGTCATCCT CATCAACTTC
    TGTCTGTCCA TCATCTCCTC CAATGCGCTT ATACTCATCG GTCAAACTCA GACACGGAAC
    AAGGTTGTTT GTACTCTCGT GGCTGCGTTT CTGCACTTTT TCTTCCTGTC CTCTTTCTGT
    TGGGTTCTAA CGGAGGCATG GCAGTCGTAC ATGGCTGTGA CCGGCCGCCT TAGAAACCGT
    ATCATACGCA AGCGTTTCCT TTGCCTTGGT TGGGGTTTAC CTGCTCTCGT TGTAGCAATT
    TCTGTTGGAT TCACCAAGGC AAAGGGATAT GGCACTGTTA ACTATTGCTG GCTGTCTCTG
    GAAGGAGGGC TGTTGTACGC ATTTGTGGGA CCAGCTGCTG CTGTCGTCCT GGTTAACATG
    GTTATCGGGA TTCTGGTTTT TAACAAACTG GTGTCCAAAG ATGGCATTAC TGATATGAAA
    CTCAAGGAGA GGGCCGGTCA GATGACAGTG CCACTGTACA ATATGACGCT CAAATGTGCC
    AAGTGCGGAG TTATCTCGTC GCCTGACGTT TCCACCACAG CTACCAGTAA CGCCATGGCG
    TCGCTGTGGA GCTCCTGCGT TGTTCTGCCG TTGCTGGCGC TCACTTGGAT GTCGGCAGTC
    TTGGCTATTA CTGACCGCCG TTCAGCCTTG TTTCAAATCC TTTTTGCCGT CTTTGACTCC
    CTCGAGGGCT TTGTTATCGT GATGGTGCAT TGCATCCTAC GCAGAGAGGT TCAAGAAGCA
    GTAAAGTGTC GTGTTGTTGA CCGTCAAGAA GATGGAAACG GTGACTCTGG TGGATCCTTC
    CAGAACGGTC ATGCTCAGCT GATGACTGAC TTTGAAAAGG ATGTGGACAT AGCTTGTCGA
    TCAGGCACCC TGAAGCGTCC CTCCCAGCAA GGCGAGGAGA AGGTTCCATC CCAGCAGATC
    ACAGTACAGA AAGGCTCCAA CTTCAACACC CTTCCTGCCA GCATGGCAAA GGTCCACCTG
    CAAAACGTAG CCAACTACGC CAGTCACACT CTCACCATGC GCCGCGACAA AGGCGCCATC
    GCCGGGGTCA GCACGGAGTT ACCCGGGGCC AAATCTATTT ACATCTGTGA CGGCGAGCTC
    TTCAAACAGC TTGACGGAGC ACCGGGAGAC ATTCCTGAAG GAGGTGGTGG AGGTGCAGGT
    CAGGGTTATG TGCTTTTGCC CAACAACAAC AACACCCTTA GGCCTACGAA GGGTGGCAAG
    GAAGACCCGG CCGCCAAATA TAACATCAGT ATCGAACAGT TACCCCAGAC AAGGCTGATC
    CACCTGGCCA ATCCTGTTGG CACCGATCCC GTGGCAGGCT TCGGGCTGAA GAGCTTGCCC
    ACCGACCGAG TCAGCGTTTC ATGCTCGGAG AGGGAGTCCC CGGTGCAGAC GGTGCAGAAT
    ATCTCCAGCG AGTCCCAGAT GACAAATACG TGTGAGCAAG GGGATTCTGG GAACTCTGGG
    ATGTCTAAGA GCGAGACTGT CTCCACACTG TCCATGAGCT CTCTGGAGAG ACGAAAATCA
    CGCTATGCAG AGCTGGACTT TGAGAAGATC ATGCACACAC GGAAGCGTCA TCAGGACATG
    TTTCAGGACC TGAATCGCAA ACTGCAGCAC GCAGAGAAGG ACAGAGACTC TCCTCCAGTC
    GACTGCAAGC CTGGAAAGAG ATGGAGCATT TCGTCAGGAG GAAGCGACAA AACCAACCTT
    AGTGACAAGC AGCAGACCCC CAGTAAGCGG GCATGGGAGG GCATCAGGAA GACTCACTCT
    CCTCCATCAT GGGTGCGCAA GGAGCTGGAG CCGCTGCAGG CCTCACCGCT GGAGATGCAG
    GCAGTGGAGT GGGAGAAGGC CAGTGCCACC ATCCCTCTAG TGGGCCAGGA GATCATGGAC
    CTGCAGACGG AGGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290827.1
    CDS747..5564
    Translation

    Target ORF information:

    RefSeq Version XM_009292552.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292552.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCT ACAGGCAGTA TTCCTCAAAA
    GGCCAGGGTT TAAGTCCAGT GAAATACAAA GGAATGCAAG AGGCCATGCT TGGCAAAGGC
    CGGCTTGGCT CTCGTAGCCA ATCCCTGCGC TCGGTGGACA GCCGTAAACG AGAGGACTCT
    GATAAGACCC GCAGCGGACA GCAGTCTTCC CAAACAGTGG ATTCTCCGTC GGGTGAGGAG
    TGGTCTGCGT GGAGCGTGTG TTCGGCCACG TGTGGTGAGG GCTGGCAGAG CCGCACACGC
    TTCTGCGTCT CCGTGTCGTA CAGCACGCAG TGCAGCGGGC CACTGCGCGA ACAGAGACCC
    TGCAATAACA CTGCTGTGTG TCCAGAACAT GGGGCGTGGG GCGAGTGGAC ACCATGGAGC
    TTGTGTTCAT CAACATGCGG CCGTGGCTAC AGGGAACGCA ACCGCTCCTG CAAGCCACCC
    CAGTTCGGAG GAAACCCCTG TGATGGTCCA GAGAAACAGA CTAAATTCTG CAACATCGCT
    GTATGCCCAG TGGATGGCGT GTGGAACGAG TGGTCTGGTT GGAGTTCCTG TTCTTCAACT
    TGTTCGAATG GAACAATGCA GCGAATTCGG GAGTGTAACG GGCCCTCGTA TGGGGGCTTA
    GAGTGTCAAG GCGAGTGGCA CGAGACACGG GATTGCTTCC TTAGAGAATG TCCTGTGGAT
    GGCCAATGGC AACTTTGGAG CACTTGGTCT GGTTGCACCA AAACCTGTGG TGGAGGAAGT
    CAGCAGAGAC AGAGGCTGTG TTATGGTCCC TTTTTTGGGG GAGAGGCATG CCCGGGGGAC
    CGAGAAGAAG TTAGACGTTG CAATGAGAAG CGATGCCCAG AGCCACATGA AATCTGTGAT
    GAGGAGAATT TCTCTAATGT AGTGTGGAAG AAAACACCTG CAGGGGACAC AGCGGCTGTC
    CGCTGCCCAC CAAATGCTGT AGGATTAATT CTACGGCGAT GTTCATTAGA CAATGAGGGT
    ATAGCATACT GGGAGAACCC AACATATATG AAGTGCGTCT CAAATGATTA CAGAAGTATC
    CAAACACTGA CCAGAGATCA CCTGTCCAAG GCGCAGCGAG GGCTTGTGGG GGATGGTGTT
    TCTGAAGTGA TGACAAAGCT GAGGGTGACG TCAAGTGATG GAACGAGCTA CAGCGGAGAT
    CTTTTGGCTA TCATAGATGT GCTAAAAAAC ATGACAGAGA TATTTAGGAG ATCCTACTAC
    AGCCCCAGTA ATGCAGACAT GAGGAATTTT GTGCAGTCTG TGAGTAACTT ACTGATGGAG
    GAAAACAGGG ACAGGTGGGA GGAAGCACAA CTGCTGGGAC CCAATGTTAA GGAGCTTTTT
    CGTCTAGTAG AGGACTTTGT GGACGTCATC AGCCTTCGCA TGAAGGACTT CCAGGATACT
    TATGAGGTCA CGGATAATCT AGTGTTGAGC ATTCACAAGC GGCCCGTGGC TGGAAATGCA
    GATATCACTT TCCCTATGAA GGGCTGGAGG GGGATGGTGG ACTGGGCTCG CAGCTCTGAG
    GACAAAGTCA GAGTGGCCAA AAACATCCTG CTCACTGGAA AACCGGAAGA AGATGACGCT
    TCAGCTTTTG TGACTGGGAT CGTCCTGTAC AGAAACTTAG GCAGTATTCT CTCTCTGCAG
    AGAAATAGCA CAGTGCTGAA CTCCAAGGTG ATCTCTGTGA CAATCAAGCC CACTCCCAGC
    TCTCTCTCGA GTCCACTAGA GATTGAGTTC TCACACCTTT ACAATGGAAC CACAAACCAA
    ACCTGCATTT CCTGGGATGA GAACGACAGC TCGTCGTTGC TGGGCTCCTG GTCTGCGCGA
    GGCTGTAGAG CCGTGCCCGT CGACTCCAGC ACAACCAAAT GCTTGTGTGA CAGACTCTCC
    ACCTTCGCCA TTTTAGCACG CCTCAATCCC GATGTGAACA TGGATAAGAC CCAGCTTCCA
    TCTGTGACTC TGATCGTGGG CTGCGGGGTC TCGTCCCTCA CCCTGCTCCT CCTCATCATC
    ATCTATGTTT CTGTTTGGAG GTACATCCGC TCAGAGCGCT CTGTCATCCT CATCAACTTC
    TGTCTGTCCA TCATCTCCTC CAATGCGCTT ATACTCATCG GTCAAACTCA GACACGGAAC
    AAGGTTGTTT GTACTCTCGT GGCTGCGTTT CTGCACTTTT TCTTCCTGTC CTCTTTCTGT
    TGGGTTCTAA CGGAGGCATG GCAGTCGTAC ATGGCTGTGA CCGGCCGCCT TAGAAACCGT
    ATCATACGCA AGCGTTTCCT TTGCCTTGGT TGGGGTTTAC CTGCTCTCGT TGTAGCAATT
    TCTGTTGGAT TCACCAAGGC AAAGGGATAT GGCACTGTTA ACTATTGCTG GCTGTCTCTG
    GAAGGAGGGC TGTTGTACGC ATTTGTGGGA CCAGCTGCTG CTGTCGTCCT GGTTAACATG
    GTTATCGGGA TTCTGGTTTT TAACAAACTG GTGTCCAAAG ATGGCATTAC TGATATGAAA
    CTCAAGGAGA GGGCCGGTCA GATGACAGTG CCACTGTACA ATATGACGCT CAAATGTGCC
    AAGTGCGGAG TTATCTCGTC GCCTGACGTT TCCACCACAG CTACCAGTAA CGCCATGGCG
    TCGCTGTGGA GCTCCTGCGT TGTTCTGCCG TTGCTGGCGC TCACTTGGAT GTCGGCAGTC
    TTGGCTATTA CTGACCGCCG TTCAGCCTTG TTTCAAATCC TTTTTGCCGT CTTTGACTCC
    CTCGAGGGCT TTGTTATCGT GATGGTGCAT TGCATCCTAC GCAGAGAGGT TCAAGAAGCA
    GTAAAGTGTC GTGTTGTTGA CCGTCAAGAA GATGGAAACG GTGACTCTGG TGGATCCTTC
    CAGAACGGTC ATGCTCAGCT GATGACTGAC TTTGAAAAGG ATGTGGACAT AGCTTGTCGA
    TCAGGCACCC TGAAGCGTCC CTCCCAGCAA GGCGAGGAGA AGGTTCCATC CCAGCAGATC
    ACAGTACAGA AAGGCTCCAA CTTCAACACC CTTCCTGCCA GCATGGCAAA GGTCCACCTG
    CAAAACGTAG CCAACTACGC CAGTCACACT CTCACCATGC GCCGCGACAA AGGCGCCATC
    GCCGGGGTCA GCACGGAGTT ACCCGGGGCC AAATCTATTT ACATCTGTGA CGGCGAGCTC
    TTCAAACAGC TTGACGGAGC ACCGGGAGAC ATTCCTGAAG GAGGTGGTGG AGGTGCAGGT
    CAGGGTTATG TGCTTTTGCC CAACAACAAC AACACCCTTA GGCCTACGAA GGGTGGCAAG
    GAAGACCCGG CCGCCAAATA TAACATCAGT ATCGAACAGT TACCCCAGAC AAGGCTGATC
    CACCTGGCCA ATCCTGTTGG CACCGATCCC GTGGCAGGCT TCGGGCTGAA GAGCTTGCCC
    ACCGACCGAG TCAGCGTTTC ATGCTCGGAG AGGGAGTCCC CGGTGCAGAC GGTGCAGAAT
    ATCTCCAGCG AGTCCCAGAT GACAAATACG TGTGAGCAAG GGGATTCTGG GAACTCTGGG
    ATGTCTAAGA GCGAGACTGT CTCCACACTG TCCATGAGCT CTCTGGAGAG ACGAAAATCA
    CGCTATGCAG AGCTGGACTT TGAGAAGATC ATGCACACAC GGAAGCGTCA TCAGGACATG
    TTTCAGGACC TGAATCGCAA ACTGCAGCAC GCAGAGAAGG ACAGAGACTC TCCTCCAGTC
    GACTGCAAGC CTGGAAAGAG ATGGAGCATT TCGTCAGGAG GAAGCGACAA AACCAACCTT
    AGTGACAAGC AGCAGACCCC CAGTAAGCGG GCATGGGAGG GCATCAGGAA GACTCACTCT
    CCTCCATCAT GGGTGCGCAA GGAGCTGGAG CCGCTGCAGG CCTCACCGCT GGAGATGCAG
    GCAGTGGAGT GGGAGAAGGC CAGTGCCACC ATCCCTCTAG TGGGCCAGGA GATCATGGAC
    CTGCAGACGG AGGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44095
    Clone ID Related Accession (Same CDS sequence) XM_009292552.3 , XM_009292552.2
    Accession Version XM_009292552.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4818bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product brain-specific angiogenesis inhibitor 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292552.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCT ACAGGCAGTA TTCCTCAAAA
    GGCCAGGGTT TAAGTCCAGT GAAATACAAA GGAATGCAAG AGGCCATGCT TGGCAAAGGC
    CGGCTTGGCT CTCGTAGCCA ATCCCTGCGC TCGGTGGACA GCCGTAAACG AGAGGACTCT
    GATAAGACCC GCAGCGGACA GCAGTCTTCC CAAACAGTGG ATTCTCCGTC GGGTGAGGAG
    TGGTCTGCGT GGAGCGTGTG TTCGGCCACG TGTGGTGAGG GCTGGCAGAG CCGCACACGC
    TTCTGCGTCT CCGTGTCGTA CAGCACGCAG TGCAGCGGGC CACTGCGCGA ACAGAGACCC
    TGCAATAACA CTGCTGTGTG TCCAGAACAT GGGGCGTGGG GCGAGTGGAC ACCATGGAGC
    TTGTGTTCAT CAACATGCGG CCGTGGCTAC AGGGAACGCA ACCGCTCCTG CAAGCCACCC
    CAGTTCGGAG GAAACCCCTG TGATGGTCCA GAGAAACAGA CTAAATTCTG CAACATCGCT
    GTATGCCCAG TGGATGGCGT GTGGAACGAG TGGTCTGGTT GGAGTTCCTG TTCTTCAACT
    TGTTCGAATG GAACAATGCA GCGAATTCGG GAGTGTAACG GGCCCTCGTA TGGGGGCTTA
    GAGTGTCAAG GCGAGTGGCA CGAGACACGG GATTGCTTCC TTAGAGAATG TCCTGTGGAT
    GGCCAATGGC AACTTTGGAG CACTTGGTCT GGTTGCACCA AAACCTGTGG TGGAGGAAGT
    CAGCAGAGAC AGAGGCTGTG TTATGGTCCC TTTTTTGGGG GAGAGGCATG CCCGGGGGAC
    CGAGAAGAAG TTAGACGTTG CAATGAGAAG CGATGCCCAG AGCCACATGA AATCTGTGAT
    GAGGAGAATT TCTCTAATGT AGTGTGGAAG AAAACACCTG CAGGGGACAC AGCGGCTGTC
    CGCTGCCCAC CAAATGCTGT AGGATTAATT CTACGGCGAT GTTCATTAGA CAATGAGGGT
    ATAGCATACT GGGAGAACCC AACATATATG AAGTGCGTCT CAAATGATTA CAGAAGTATC
    CAAACACTGA CCAGAGATCA CCTGTCCAAG GCGCAGCGAG GGCTTGTGGG GGATGGTGTT
    TCTGAAGTGA TGACAAAGCT GAGGGTGACG TCAAGTGATG GAACGAGCTA CAGCGGAGAT
    CTTTTGGCTA TCATAGATGT GCTAAAAAAC ATGACAGAGA TATTTAGGAG ATCCTACTAC
    AGCCCCAGTA ATGCAGACAT GAGGAATTTT GTGCAGTCTG TGAGTAACTT ACTGATGGAG
    GAAAACAGGG ACAGGTGGGA GGAAGCACAA CTGCTGGGAC CCAATGTTAA GGAGCTTTTT
    CGTCTAGTAG AGGACTTTGT GGACGTCATC AGCCTTCGCA TGAAGGACTT CCAGGATACT
    TATGAGGTCA CGGATAATCT AGTGTTGAGC ATTCACAAGC GGCCCGTGGC TGGAAATGCA
    GATATCACTT TCCCTATGAA GGGCTGGAGG GGGATGGTGG ACTGGGCTCG CAGCTCTGAG
    GACAAAGTCA GAGTGGCCAA AAACATCCTG CTCACTGGAA AACCGGAAGA AGATGACGCT
    TCAGCTTTTG TGACTGGGAT CGTCCTGTAC AGAAACTTAG GCAGTATTCT CTCTCTGCAG
    AGAAATAGCA CAGTGCTGAA CTCCAAGGTG ATCTCTGTGA CAATCAAGCC CACTCCCAGC
    TCTCTCTCGA GTCCACTAGA GATTGAGTTC TCACACCTTT ACAATGGAAC CACAAACCAA
    ACCTGCATTT CCTGGGATGA GAACGACAGC TCGTCGTTGC TGGGCTCCTG GTCTGCGCGA
    GGCTGTAGAG CCGTGCCCGT CGACTCCAGC ACAACCAAAT GCTTGTGTGA CAGACTCTCC
    ACCTTCGCCA TTTTAGCACG CCTCAATCCC GATGTGAACA TGGATAAGAC CCAGCTTCCA
    TCTGTGACTC TGATCGTGGG CTGCGGGGTC TCGTCCCTCA CCCTGCTCCT CCTCATCATC
    ATCTATGTTT CTGTTTGGAG GTACATCCGC TCAGAGCGCT CTGTCATCCT CATCAACTTC
    TGTCTGTCCA TCATCTCCTC CAATGCGCTT ATACTCATCG GTCAAACTCA GACACGGAAC
    AAGGTTGTTT GTACTCTCGT GGCTGCGTTT CTGCACTTTT TCTTCCTGTC CTCTTTCTGT
    TGGGTTCTAA CGGAGGCATG GCAGTCGTAC ATGGCTGTGA CCGGCCGCCT TAGAAACCGT
    ATCATACGCA AGCGTTTCCT TTGCCTTGGT TGGGGTTTAC CTGCTCTCGT TGTAGCAATT
    TCTGTTGGAT TCACCAAGGC AAAGGGATAT GGCACTGTTA ACTATTGCTG GCTGTCTCTG
    GAAGGAGGGC TGTTGTACGC ATTTGTGGGA CCAGCTGCTG CTGTCGTCCT GGTTAACATG
    GTTATCGGGA TTCTGGTTTT TAACAAACTG GTGTCCAAAG ATGGCATTAC TGATATGAAA
    CTCAAGGAGA GGGCCGGTCA GATGACAGTG CCACTGTACA ATATGACGCT CAAATGTGCC
    AAGTGCGGAG TTATCTCGTC GCCTGACGTT TCCACCACAG CTACCAGTAA CGCCATGGCG
    TCGCTGTGGA GCTCCTGCGT TGTTCTGCCG TTGCTGGCGC TCACTTGGAT GTCGGCAGTC
    TTGGCTATTA CTGACCGCCG TTCAGCCTTG TTTCAAATCC TTTTTGCCGT CTTTGACTCC
    CTCGAGGGCT TTGTTATCGT GATGGTGCAT TGCATCCTAC GCAGAGAGGT TCAAGAAGCA
    GTAAAGTGTC GTGTTGTTGA CCGTCAAGAA GATGGAAACG GTGACTCTGG TGGATCCTTC
    CAGAACGGTC ATGCTCAGCT GATGACTGAC TTTGAAAAGG ATGTGGACAT AGCTTGTCGA
    TCAGGCACCC TGAAGCGTCC CTCCCAGCAA GGCGAGGAGA AGGTTCCATC CCAGCAGATC
    ACAGTACAGA AAGGCTCCAA CTTCAACACC CTTCCTGCCA GCATGGCAAA GGTCCACCTG
    CAAAACGTAG CCAACTACGC CAGTCACACT CTCACCATGC GCCGCGACAA AGGCGCCATC
    GCCGGGGTCA GCACGGAGTT ACCCGGGGCC AAATCTATTT ACATCTGTGA CGGCGAGCTC
    TTCAAACAGC TTGACGGAGC ACCGGGAGAC ATTCCTGAAG GAGGTGGTGG AGGTGCAGGT
    CAGGGTTATG TGCTTTTGCC CAACAACAAC AACACCCTTA GGCCTACGAA GGGTGGCAAG
    GAAGACCCGG CCGCCAAATA TAACATCAGT ATCGAACAGT TACCCCAGAC AAGGCTGATC
    CACCTGGCCA ATCCTGTTGG CACCGATCCC GTGGCAGGCT TCGGGCTGAA GAGCTTGCCC
    ACCGACCGAG TCAGCGTTTC ATGCTCGGAG AGGGAGTCCC CGGTGCAGAC GGTGCAGAAT
    ATCTCCAGCG AGTCCCAGAT GACAAATACG TGTGAGCAAG GGGATTCTGG GAACTCTGGG
    ATGTCTAAGA GCGAGACTGT CTCCACACTG TCCATGAGCT CTCTGGAGAG ACGAAAATCA
    CGCTATGCAG AGCTGGACTT TGAGAAGATC ATGCACACAC GGAAGCGTCA TCAGGACATG
    TTTCAGGACC TGAATCGCAA ACTGCAGCAC GCAGAGAAGG ACAGAGACTC TCCTCCAGTC
    GACTGCAAGC CTGGAAAGAG ATGGAGCATT TCGTCAGGAG GAAGCGACAA AACCAACCTT
    AGTGACAAGC AGCAGACCCC CAGTAAGCGG GCATGGGAGG GCATCAGGAA GACTCACTCT
    CCTCCATCAT GGGTGCGCAA GGAGCTGGAG CCGCTGCAGG CCTCACCGCT GGAGATGCAG
    GCAGTGGAGT GGGAGAAGGC CAGTGCCACC ATCCCTCTAG TGGGCCAGGA GATCATGGAC
    CTGCAGACGG AGGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290827.1
    CDS731..5548
    Translation

    Target ORF information:

    RefSeq Version XM_009292552.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292552.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCT ACAGGCAGTA TTCCTCAAAA
    GGCCAGGGTT TAAGTCCAGT GAAATACAAA GGAATGCAAG AGGCCATGCT TGGCAAAGGC
    CGGCTTGGCT CTCGTAGCCA ATCCCTGCGC TCGGTGGACA GCCGTAAACG AGAGGACTCT
    GATAAGACCC GCAGCGGACA GCAGTCTTCC CAAACAGTGG ATTCTCCGTC GGGTGAGGAG
    TGGTCTGCGT GGAGCGTGTG TTCGGCCACG TGTGGTGAGG GCTGGCAGAG CCGCACACGC
    TTCTGCGTCT CCGTGTCGTA CAGCACGCAG TGCAGCGGGC CACTGCGCGA ACAGAGACCC
    TGCAATAACA CTGCTGTGTG TCCAGAACAT GGGGCGTGGG GCGAGTGGAC ACCATGGAGC
    TTGTGTTCAT CAACATGCGG CCGTGGCTAC AGGGAACGCA ACCGCTCCTG CAAGCCACCC
    CAGTTCGGAG GAAACCCCTG TGATGGTCCA GAGAAACAGA CTAAATTCTG CAACATCGCT
    GTATGCCCAG TGGATGGCGT GTGGAACGAG TGGTCTGGTT GGAGTTCCTG TTCTTCAACT
    TGTTCGAATG GAACAATGCA GCGAATTCGG GAGTGTAACG GGCCCTCGTA TGGGGGCTTA
    GAGTGTCAAG GCGAGTGGCA CGAGACACGG GATTGCTTCC TTAGAGAATG TCCTGTGGAT
    GGCCAATGGC AACTTTGGAG CACTTGGTCT GGTTGCACCA AAACCTGTGG TGGAGGAAGT
    CAGCAGAGAC AGAGGCTGTG TTATGGTCCC TTTTTTGGGG GAGAGGCATG CCCGGGGGAC
    CGAGAAGAAG TTAGACGTTG CAATGAGAAG CGATGCCCAG AGCCACATGA AATCTGTGAT
    GAGGAGAATT TCTCTAATGT AGTGTGGAAG AAAACACCTG CAGGGGACAC AGCGGCTGTC
    CGCTGCCCAC CAAATGCTGT AGGATTAATT CTACGGCGAT GTTCATTAGA CAATGAGGGT
    ATAGCATACT GGGAGAACCC AACATATATG AAGTGCGTCT CAAATGATTA CAGAAGTATC
    CAAACACTGA CCAGAGATCA CCTGTCCAAG GCGCAGCGAG GGCTTGTGGG GGATGGTGTT
    TCTGAAGTGA TGACAAAGCT GAGGGTGACG TCAAGTGATG GAACGAGCTA CAGCGGAGAT
    CTTTTGGCTA TCATAGATGT GCTAAAAAAC ATGACAGAGA TATTTAGGAG ATCCTACTAC
    AGCCCCAGTA ATGCAGACAT GAGGAATTTT GTGCAGTCTG TGAGTAACTT ACTGATGGAG
    GAAAACAGGG ACAGGTGGGA GGAAGCACAA CTGCTGGGAC CCAATGTTAA GGAGCTTTTT
    CGTCTAGTAG AGGACTTTGT GGACGTCATC AGCCTTCGCA TGAAGGACTT CCAGGATACT
    TATGAGGTCA CGGATAATCT AGTGTTGAGC ATTCACAAGC GGCCCGTGGC TGGAAATGCA
    GATATCACTT TCCCTATGAA GGGCTGGAGG GGGATGGTGG ACTGGGCTCG CAGCTCTGAG
    GACAAAGTCA GAGTGGCCAA AAACATCCTG CTCACTGGAA AACCGGAAGA AGATGACGCT
    TCAGCTTTTG TGACTGGGAT CGTCCTGTAC AGAAACTTAG GCAGTATTCT CTCTCTGCAG
    AGAAATAGCA CAGTGCTGAA CTCCAAGGTG ATCTCTGTGA CAATCAAGCC CACTCCCAGC
    TCTCTCTCGA GTCCACTAGA GATTGAGTTC TCACACCTTT ACAATGGAAC CACAAACCAA
    ACCTGCATTT CCTGGGATGA GAACGACAGC TCGTCGTTGC TGGGCTCCTG GTCTGCGCGA
    GGCTGTAGAG CCGTGCCCGT CGACTCCAGC ACAACCAAAT GCTTGTGTGA CAGACTCTCC
    ACCTTCGCCA TTTTAGCACG CCTCAATCCC GATGTGAACA TGGATAAGAC CCAGCTTCCA
    TCTGTGACTC TGATCGTGGG CTGCGGGGTC TCGTCCCTCA CCCTGCTCCT CCTCATCATC
    ATCTATGTTT CTGTTTGGAG GTACATCCGC TCAGAGCGCT CTGTCATCCT CATCAACTTC
    TGTCTGTCCA TCATCTCCTC CAATGCGCTT ATACTCATCG GTCAAACTCA GACACGGAAC
    AAGGTTGTTT GTACTCTCGT GGCTGCGTTT CTGCACTTTT TCTTCCTGTC CTCTTTCTGT
    TGGGTTCTAA CGGAGGCATG GCAGTCGTAC ATGGCTGTGA CCGGCCGCCT TAGAAACCGT
    ATCATACGCA AGCGTTTCCT TTGCCTTGGT TGGGGTTTAC CTGCTCTCGT TGTAGCAATT
    TCTGTTGGAT TCACCAAGGC AAAGGGATAT GGCACTGTTA ACTATTGCTG GCTGTCTCTG
    GAAGGAGGGC TGTTGTACGC ATTTGTGGGA CCAGCTGCTG CTGTCGTCCT GGTTAACATG
    GTTATCGGGA TTCTGGTTTT TAACAAACTG GTGTCCAAAG ATGGCATTAC TGATATGAAA
    CTCAAGGAGA GGGCCGGTCA GATGACAGTG CCACTGTACA ATATGACGCT CAAATGTGCC
    AAGTGCGGAG TTATCTCGTC GCCTGACGTT TCCACCACAG CTACCAGTAA CGCCATGGCG
    TCGCTGTGGA GCTCCTGCGT TGTTCTGCCG TTGCTGGCGC TCACTTGGAT GTCGGCAGTC
    TTGGCTATTA CTGACCGCCG TTCAGCCTTG TTTCAAATCC TTTTTGCCGT CTTTGACTCC
    CTCGAGGGCT TTGTTATCGT GATGGTGCAT TGCATCCTAC GCAGAGAGGT TCAAGAAGCA
    GTAAAGTGTC GTGTTGTTGA CCGTCAAGAA GATGGAAACG GTGACTCTGG TGGATCCTTC
    CAGAACGGTC ATGCTCAGCT GATGACTGAC TTTGAAAAGG ATGTGGACAT AGCTTGTCGA
    TCAGGCACCC TGAAGCGTCC CTCCCAGCAA GGCGAGGAGA AGGTTCCATC CCAGCAGATC
    ACAGTACAGA AAGGCTCCAA CTTCAACACC CTTCCTGCCA GCATGGCAAA GGTCCACCTG
    CAAAACGTAG CCAACTACGC CAGTCACACT CTCACCATGC GCCGCGACAA AGGCGCCATC
    GCCGGGGTCA GCACGGAGTT ACCCGGGGCC AAATCTATTT ACATCTGTGA CGGCGAGCTC
    TTCAAACAGC TTGACGGAGC ACCGGGAGAC ATTCCTGAAG GAGGTGGTGG AGGTGCAGGT
    CAGGGTTATG TGCTTTTGCC CAACAACAAC AACACCCTTA GGCCTACGAA GGGTGGCAAG
    GAAGACCCGG CCGCCAAATA TAACATCAGT ATCGAACAGT TACCCCAGAC AAGGCTGATC
    CACCTGGCCA ATCCTGTTGG CACCGATCCC GTGGCAGGCT TCGGGCTGAA GAGCTTGCCC
    ACCGACCGAG TCAGCGTTTC ATGCTCGGAG AGGGAGTCCC CGGTGCAGAC GGTGCAGAAT
    ATCTCCAGCG AGTCCCAGAT GACAAATACG TGTGAGCAAG GGGATTCTGG GAACTCTGGG
    ATGTCTAAGA GCGAGACTGT CTCCACACTG TCCATGAGCT CTCTGGAGAG ACGAAAATCA
    CGCTATGCAG AGCTGGACTT TGAGAAGATC ATGCACACAC GGAAGCGTCA TCAGGACATG
    TTTCAGGACC TGAATCGCAA ACTGCAGCAC GCAGAGAAGG ACAGAGACTC TCCTCCAGTC
    GACTGCAAGC CTGGAAAGAG ATGGAGCATT TCGTCAGGAG GAAGCGACAA AACCAACCTT
    AGTGACAAGC AGCAGACCCC CAGTAAGCGG GCATGGGAGG GCATCAGGAA GACTCACTCT
    CCTCCATCAT GGGTGCGCAA GGAGCTGGAG CCGCTGCAGG CCTCACCGCT GGAGATGCAG
    GCAGTGGAGT GGGAGAAGGC CAGTGCCACC ATCCCTCTAG TGGGCCAGGA GATCATGGAC
    CTGCAGACGG AGGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44096
    Clone ID Related Accession (Same CDS sequence) XM_009292553.2 , XM_009292553.3
    Accession Version XM_009292553.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4797bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product brain-specific angiogenesis inhibitor 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292553.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCT ACAGGCAGTA TTCCTCAAAA
    GGCCAGGGTT TAAGTCCAGT GAAATACAAA GGAATGCAAG AGGCCATGCT TGGCAAAGGC
    CGGCTTGGCT CTCGTAGCCA ATCCCTGCGC TCGGTGGACA GCCGTAAACG AGAGGACTCT
    GATAAGACCC GCAGCGGACA GCAGTCTTCC CAAACAGTGG ATTCTCCGTC GGGTGAGGAG
    TGGTCTGCGT GGAGCGTGTG TTCGGCCACG TGTGGTGAGG GCTGGCAGAG CCGCACACGC
    TTCTGCGTCT CCGTGTCGTA CAGCACGCAG TGCAGCGGGC CACTGCGCGA ACAGAGACCC
    TGCAATAACA CTGCTGTGTG TCCAGCCGCT CTGATTACAG AACATGGGGC GTGGGGCGAG
    TGGACACCAT GGAGCTTGTG TTCATCAACA TGCGGCCGTG GCTACAGGGA ACGCAACCGC
    TCCTGCAAGC CACCCCAGTT CGGAGGAAAC CCCTGTGATG GTCCAGAGAA ACAGACTAAA
    TTCTGCAACA TCGCTGTATG CCCAGTGGAT GGCGTGTGGA ACGAGTGGTC TGGTTGGAGT
    TCCTGTTCTT CAACTTGTTC GAATGGAACA ATGCAGCGAA TTCGGGAGTG TAACGGGCCC
    TCGTATGGGG GCTTAGAGTG TCAAGGCGAG TGGCACGAGA CACGGGATTG CTTCCTTAGA
    GAATGTCCTG TGGATGGCCA ATGGCAACTT TGGAGCACTT GGTCTGGTTG CACCAAAACC
    TGTGGTGGAG GAAGTCAGCA GAGACAGAGG CTGTGTTATG GTCCCTTTTT TGGGGGAGAG
    GCATGCCCGG GGGACCGAGA AGAAGTTAGA CGTTGCAATG AGAAGCGATG CCCAGAGCCA
    CATGAAATCT GTGATGAGGA GAATTTCTCT AATGTAGTGT GGAAGAAAAC ACCTGCAGGG
    GACACAGCGG CTGTCCGCTG CCCACCAAAT GCTGTAGGAT TAATTCTACG GCGATGTTCA
    TTAGACAATG AGGGTATAGC ATACTGGGAG AACCCAACAT ATATGAAGTG CGTCTCAAAT
    GATTACAGAA GTATCCAAAC ACTGACCAGA GATCACCTGT CCAAGGCGCA GCGAGGGCTT
    GTGGGGGATG GTGTTTCTGA AGTGATGACA AAGCTGAGGG TGACGTCAAG TGATGGAACG
    AGCTACAGCG GAGATCTTTT GGCTATCATA GATGTGCTAA AAAACATGAC AGAGATATTT
    AGGAGATCCT ACTACAGCCC CAGTAATGCA GACATGAGGA ATTTTGTGCA GTCTGTGAGT
    AACTTACTGA TGGAGGAAAA CAGGGACAGG TGGGAGGAAG CACAACTGCT GGGACCCAAT
    GTTAAGGAGC TTTTTCGTCT AGTAGAGGAC TTTGTGGACG TCATCAGCCT TCGCATGAAG
    GACTTCCAGG ATACTTATGA GGTCACGGAT AATCTAGTGT TGAGCATTCA CAAGCGGCCC
    GTGGCTGGAA ATGCAGATAT CACTTTCCCT ATGAAGGGCT GGAGGGGGAT GGTGGACTGG
    GCTCGCAGCT CTGAGGACAA AGTCAGAGTG GCCAAAAACA TCCTGCTCAC TGGAAAACCG
    GAAGAAGATG ACGCTTCAGC TTTTGTGACT GGGATCGTCC TGTACAGAAA CTTAGGCAGT
    ATTCTCTCTC TGCAGAGAAA TAGCACAGTG CTGAACTCCA AGGTGATCTC TGTGACAATC
    AAGCCCACTC CCAGCTCTCT CTCGAGTCCA CTAGAGATTG AGTTCTCACA CCTTTACAAT
    GGAACCACAA ACCAAACCTG CATTTCCTGG GATGAGAACG ACAGCTCGTC GTTGCTGGGC
    TCCTGGTCTG CGCGAGGCTG TAGAGCCGTG CCCGTCGACT CCAGCACAAC CAAATGCTTG
    TGTGACAGAC TCTCCACCTT CGCCATTTTA GCACGCCTCA ATCCCGATGT GAACATGGAT
    AAGACCCAGC TTCCATCTGT GACTCTGATC GTGGGCTGCG GGGTCTCGTC CCTCACCCTG
    CTCCTCCTCA TCATCATCTA TGTTTCTGTT TGGAGGTACA TCCGCTCAGA GCGCTCTGTC
    ATCCTCATCA ACTTCTGTCT GTCCATCATC TCCTCCAATG CGCTTATACT CATCGGTCAA
    ACTCAGACAC GGAACAAGGT TGTTTGTACT CTCGTGGCTG CGTTTCTGCA CTTTTTCTTC
    CTGTCCTCTT TCTGTTGGGT TCTAACGGAG GCATGGCAGT CGTACATGGC TGTGACCGGC
    CGCCTTAGAA ACCGTATCAT ACGCAAGCGT TTCCTTTGCC TTGGTTGGGG TTTACCTGCT
    CTCGTTGTAG CAATTTCTGT TGGATTCACC AAGGCAAAGG GATATGGCAC TGTTAACTAT
    TGCTGGCTGT CTCTGGAAGG AGGGCTGTTG TACGCATTTG TGGGACCAGC TGCTGCTGTC
    GTCCTGGTTA ACATGGTTAT CGGGATTCTG GTTTTTAACA AACTGGTGTC CAAAGATGGC
    ATTACTGATA TGAAACTCAA GGAGAGGGCC GGTCAGATGA CAGTGCCACT GTACAATATG
    ACGCTCAAAT GTGCCAAGTG CGGAGTTATC TCGTCGCCTG ACGTTTCCAC CACAGCTACC
    AGTAACGCCA TGGCGTCGCT GTGGAGCTCC TGCGTTGTTC TGCCGTTGCT GGCGCTCACT
    TGGATGTCGG CAGTCTTGGC TATTACTGAC CGCCGTTCAG CCTTGTTTCA AATCCTTTTT
    GCCGTCTTTG ACTCCCTCGA GGGCTTTGTT ATCGTGATGG TGCATTGCAT CCTACGCAGA
    GAGGTTCAAG AAGCAGTAAA GTGTCGTGTT GTTGACCGTC AAGAAGATGG AAACGGTGAC
    TCTGGTGGAT CCTTCCAGAA CGGTCATGCT CAGCTGATGA CTGACTTTGA AAAGGATGTG
    GACATAGCTT GTCGATCAGG CACCCTGAAG CGTCCCTCCC AGCAAGGCGA GGAGAAGGTT
    CCATCCCAGC AGATCACAGT ACAGAAAGGC TCCAACTTCA ACACCCTTCC TGCCAGCATG
    GCAAAGGTCC ACCTGCAAAA CGTAGCCAAC TACGCCAGTC ACACTCTCAC CATGCGCCGC
    GACAAAGGCG CCATCGCCGG GGTCAGCACG GAGTTACCCG GGGCCAAATC TATTTACATC
    TGTGACGGCG AGCTCTTCAA ACAGCTTGAC GGAGCACCGG GAGACATTCC TGAAGGAGGT
    GGTGGAGGTG CAGGTCAGGG TTATGTGCTT TTGCCCAACA ACAACAACAC CCTTAGGCCT
    ACGAAGGGTG GCAAGGAAGA CCCGGCCGCC AAATATAACA TCAGTATCGA ACAGTTACCC
    CAGACAAGGC TGATCCACCT GGCCAATCCT GTTGGCACCG ATCCCGTGGC AGGCTTCGGG
    CTGAAGAGCT TGCCCACCGA CCGAGTCAGC GTTTCATGCT CGGAGAGGGA GTCCCCGGTG
    CAGACGGTGC AGAATATCTC CAGCGAGTCC CAGATGACAA ATACGTGTGA GCAAGGGGAT
    TCTGGGAACT CTGGGATGTC TAAGAGCGAG ACTGTCTCCA CACTGTCCAT GAGCTCTCTG
    GAGAAGATCA TGCACACACG GAAGCGTCAT CAGGACATGT TTCAGGACCT GAATCGCAAA
    CTGCAGCACG CAGAGAAGGA CAGAGACTCT CCTCCAGTCG ACTGCAAGCC TGGAAAGAGA
    TGGAGCATTT CGTCAGGAGG AAGCGACAAA ACCAACCTTA GTGACAAGCA GCAGACCCCC
    AGTAAGCGGG CATGGGAGGG CATCAGGAAG ACTCACTCTC CTCCATCATG GGTGCGCAAG
    GAGCTGGAGC CGCTGCAGGC CTCACCGCTG GAGATGCAGG CAGTGGAGTG GGAGAAGGCC
    AGTGCCACCA TCCCTCTAGT GGGCCAGGAG ATCATGGACC TGCAGACGGA GGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290828.1
    CDS731..5527
    Translation

    Target ORF information:

    RefSeq Version XM_009292553.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292553.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCT ACAGGCAGTA TTCCTCAAAA
    GGCCAGGGTT TAAGTCCAGT GAAATACAAA GGAATGCAAG AGGCCATGCT TGGCAAAGGC
    CGGCTTGGCT CTCGTAGCCA ATCCCTGCGC TCGGTGGACA GCCGTAAACG AGAGGACTCT
    GATAAGACCC GCAGCGGACA GCAGTCTTCC CAAACAGTGG ATTCTCCGTC GGGTGAGGAG
    TGGTCTGCGT GGAGCGTGTG TTCGGCCACG TGTGGTGAGG GCTGGCAGAG CCGCACACGC
    TTCTGCGTCT CCGTGTCGTA CAGCACGCAG TGCAGCGGGC CACTGCGCGA ACAGAGACCC
    TGCAATAACA CTGCTGTGTG TCCAGCCGCT CTGATTACAG AACATGGGGC GTGGGGCGAG
    TGGACACCAT GGAGCTTGTG TTCATCAACA TGCGGCCGTG GCTACAGGGA ACGCAACCGC
    TCCTGCAAGC CACCCCAGTT CGGAGGAAAC CCCTGTGATG GTCCAGAGAA ACAGACTAAA
    TTCTGCAACA TCGCTGTATG CCCAGTGGAT GGCGTGTGGA ACGAGTGGTC TGGTTGGAGT
    TCCTGTTCTT CAACTTGTTC GAATGGAACA ATGCAGCGAA TTCGGGAGTG TAACGGGCCC
    TCGTATGGGG GCTTAGAGTG TCAAGGCGAG TGGCACGAGA CACGGGATTG CTTCCTTAGA
    GAATGTCCTG TGGATGGCCA ATGGCAACTT TGGAGCACTT GGTCTGGTTG CACCAAAACC
    TGTGGTGGAG GAAGTCAGCA GAGACAGAGG CTGTGTTATG GTCCCTTTTT TGGGGGAGAG
    GCATGCCCGG GGGACCGAGA AGAAGTTAGA CGTTGCAATG AGAAGCGATG CCCAGAGCCA
    CATGAAATCT GTGATGAGGA GAATTTCTCT AATGTAGTGT GGAAGAAAAC ACCTGCAGGG
    GACACAGCGG CTGTCCGCTG CCCACCAAAT GCTGTAGGAT TAATTCTACG GCGATGTTCA
    TTAGACAATG AGGGTATAGC ATACTGGGAG AACCCAACAT ATATGAAGTG CGTCTCAAAT
    GATTACAGAA GTATCCAAAC ACTGACCAGA GATCACCTGT CCAAGGCGCA GCGAGGGCTT
    GTGGGGGATG GTGTTTCTGA AGTGATGACA AAGCTGAGGG TGACGTCAAG TGATGGAACG
    AGCTACAGCG GAGATCTTTT GGCTATCATA GATGTGCTAA AAAACATGAC AGAGATATTT
    AGGAGATCCT ACTACAGCCC CAGTAATGCA GACATGAGGA ATTTTGTGCA GTCTGTGAGT
    AACTTACTGA TGGAGGAAAA CAGGGACAGG TGGGAGGAAG CACAACTGCT GGGACCCAAT
    GTTAAGGAGC TTTTTCGTCT AGTAGAGGAC TTTGTGGACG TCATCAGCCT TCGCATGAAG
    GACTTCCAGG ATACTTATGA GGTCACGGAT AATCTAGTGT TGAGCATTCA CAAGCGGCCC
    GTGGCTGGAA ATGCAGATAT CACTTTCCCT ATGAAGGGCT GGAGGGGGAT GGTGGACTGG
    GCTCGCAGCT CTGAGGACAA AGTCAGAGTG GCCAAAAACA TCCTGCTCAC TGGAAAACCG
    GAAGAAGATG ACGCTTCAGC TTTTGTGACT GGGATCGTCC TGTACAGAAA CTTAGGCAGT
    ATTCTCTCTC TGCAGAGAAA TAGCACAGTG CTGAACTCCA AGGTGATCTC TGTGACAATC
    AAGCCCACTC CCAGCTCTCT CTCGAGTCCA CTAGAGATTG AGTTCTCACA CCTTTACAAT
    GGAACCACAA ACCAAACCTG CATTTCCTGG GATGAGAACG ACAGCTCGTC GTTGCTGGGC
    TCCTGGTCTG CGCGAGGCTG TAGAGCCGTG CCCGTCGACT CCAGCACAAC CAAATGCTTG
    TGTGACAGAC TCTCCACCTT CGCCATTTTA GCACGCCTCA ATCCCGATGT GAACATGGAT
    AAGACCCAGC TTCCATCTGT GACTCTGATC GTGGGCTGCG GGGTCTCGTC CCTCACCCTG
    CTCCTCCTCA TCATCATCTA TGTTTCTGTT TGGAGGTACA TCCGCTCAGA GCGCTCTGTC
    ATCCTCATCA ACTTCTGTCT GTCCATCATC TCCTCCAATG CGCTTATACT CATCGGTCAA
    ACTCAGACAC GGAACAAGGT TGTTTGTACT CTCGTGGCTG CGTTTCTGCA CTTTTTCTTC
    CTGTCCTCTT TCTGTTGGGT TCTAACGGAG GCATGGCAGT CGTACATGGC TGTGACCGGC
    CGCCTTAGAA ACCGTATCAT ACGCAAGCGT TTCCTTTGCC TTGGTTGGGG TTTACCTGCT
    CTCGTTGTAG CAATTTCTGT TGGATTCACC AAGGCAAAGG GATATGGCAC TGTTAACTAT
    TGCTGGCTGT CTCTGGAAGG AGGGCTGTTG TACGCATTTG TGGGACCAGC TGCTGCTGTC
    GTCCTGGTTA ACATGGTTAT CGGGATTCTG GTTTTTAACA AACTGGTGTC CAAAGATGGC
    ATTACTGATA TGAAACTCAA GGAGAGGGCC GGTCAGATGA CAGTGCCACT GTACAATATG
    ACGCTCAAAT GTGCCAAGTG CGGAGTTATC TCGTCGCCTG ACGTTTCCAC CACAGCTACC
    AGTAACGCCA TGGCGTCGCT GTGGAGCTCC TGCGTTGTTC TGCCGTTGCT GGCGCTCACT
    TGGATGTCGG CAGTCTTGGC TATTACTGAC CGCCGTTCAG CCTTGTTTCA AATCCTTTTT
    GCCGTCTTTG ACTCCCTCGA GGGCTTTGTT ATCGTGATGG TGCATTGCAT CCTACGCAGA
    GAGGTTCAAG AAGCAGTAAA GTGTCGTGTT GTTGACCGTC AAGAAGATGG AAACGGTGAC
    TCTGGTGGAT CCTTCCAGAA CGGTCATGCT CAGCTGATGA CTGACTTTGA AAAGGATGTG
    GACATAGCTT GTCGATCAGG CACCCTGAAG CGTCCCTCCC AGCAAGGCGA GGAGAAGGTT
    CCATCCCAGC AGATCACAGT ACAGAAAGGC TCCAACTTCA ACACCCTTCC TGCCAGCATG
    GCAAAGGTCC ACCTGCAAAA CGTAGCCAAC TACGCCAGTC ACACTCTCAC CATGCGCCGC
    GACAAAGGCG CCATCGCCGG GGTCAGCACG GAGTTACCCG GGGCCAAATC TATTTACATC
    TGTGACGGCG AGCTCTTCAA ACAGCTTGAC GGAGCACCGG GAGACATTCC TGAAGGAGGT
    GGTGGAGGTG CAGGTCAGGG TTATGTGCTT TTGCCCAACA ACAACAACAC CCTTAGGCCT
    ACGAAGGGTG GCAAGGAAGA CCCGGCCGCC AAATATAACA TCAGTATCGA ACAGTTACCC
    CAGACAAGGC TGATCCACCT GGCCAATCCT GTTGGCACCG ATCCCGTGGC AGGCTTCGGG
    CTGAAGAGCT TGCCCACCGA CCGAGTCAGC GTTTCATGCT CGGAGAGGGA GTCCCCGGTG
    CAGACGGTGC AGAATATCTC CAGCGAGTCC CAGATGACAA ATACGTGTGA GCAAGGGGAT
    TCTGGGAACT CTGGGATGTC TAAGAGCGAG ACTGTCTCCA CACTGTCCAT GAGCTCTCTG
    GAGAAGATCA TGCACACACG GAAGCGTCAT CAGGACATGT TTCAGGACCT GAATCGCAAA
    CTGCAGCACG CAGAGAAGGA CAGAGACTCT CCTCCAGTCG ACTGCAAGCC TGGAAAGAGA
    TGGAGCATTT CGTCAGGAGG AAGCGACAAA ACCAACCTTA GTGACAAGCA GCAGACCCCC
    AGTAAGCGGG CATGGGAGGG CATCAGGAAG ACTCACTCTC CTCCATCATG GGTGCGCAAG
    GAGCTGGAGC CGCTGCAGGC CTCACCGCTG GAGATGCAGG CAGTGGAGTG GGAGAAGGCC
    AGTGCCACCA TCCCTCTAGT GGGCCAGGAG ATCATGGACC TGCAGACGGA GGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44096
    Clone ID Related Accession (Same CDS sequence) XM_009292553.2 , XM_009292553.3
    Accession Version XM_009292553.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4797bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product brain-specific angiogenesis inhibitor 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009292553.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCT ACAGGCAGTA TTCCTCAAAA
    GGCCAGGGTT TAAGTCCAGT GAAATACAAA GGAATGCAAG AGGCCATGCT TGGCAAAGGC
    CGGCTTGGCT CTCGTAGCCA ATCCCTGCGC TCGGTGGACA GCCGTAAACG AGAGGACTCT
    GATAAGACCC GCAGCGGACA GCAGTCTTCC CAAACAGTGG ATTCTCCGTC GGGTGAGGAG
    TGGTCTGCGT GGAGCGTGTG TTCGGCCACG TGTGGTGAGG GCTGGCAGAG CCGCACACGC
    TTCTGCGTCT CCGTGTCGTA CAGCACGCAG TGCAGCGGGC CACTGCGCGA ACAGAGACCC
    TGCAATAACA CTGCTGTGTG TCCAGCCGCT CTGATTACAG AACATGGGGC GTGGGGCGAG
    TGGACACCAT GGAGCTTGTG TTCATCAACA TGCGGCCGTG GCTACAGGGA ACGCAACCGC
    TCCTGCAAGC CACCCCAGTT CGGAGGAAAC CCCTGTGATG GTCCAGAGAA ACAGACTAAA
    TTCTGCAACA TCGCTGTATG CCCAGTGGAT GGCGTGTGGA ACGAGTGGTC TGGTTGGAGT
    TCCTGTTCTT CAACTTGTTC GAATGGAACA ATGCAGCGAA TTCGGGAGTG TAACGGGCCC
    TCGTATGGGG GCTTAGAGTG TCAAGGCGAG TGGCACGAGA CACGGGATTG CTTCCTTAGA
    GAATGTCCTG TGGATGGCCA ATGGCAACTT TGGAGCACTT GGTCTGGTTG CACCAAAACC
    TGTGGTGGAG GAAGTCAGCA GAGACAGAGG CTGTGTTATG GTCCCTTTTT TGGGGGAGAG
    GCATGCCCGG GGGACCGAGA AGAAGTTAGA CGTTGCAATG AGAAGCGATG CCCAGAGCCA
    CATGAAATCT GTGATGAGGA GAATTTCTCT AATGTAGTGT GGAAGAAAAC ACCTGCAGGG
    GACACAGCGG CTGTCCGCTG CCCACCAAAT GCTGTAGGAT TAATTCTACG GCGATGTTCA
    TTAGACAATG AGGGTATAGC ATACTGGGAG AACCCAACAT ATATGAAGTG CGTCTCAAAT
    GATTACAGAA GTATCCAAAC ACTGACCAGA GATCACCTGT CCAAGGCGCA GCGAGGGCTT
    GTGGGGGATG GTGTTTCTGA AGTGATGACA AAGCTGAGGG TGACGTCAAG TGATGGAACG
    AGCTACAGCG GAGATCTTTT GGCTATCATA GATGTGCTAA AAAACATGAC AGAGATATTT
    AGGAGATCCT ACTACAGCCC CAGTAATGCA GACATGAGGA ATTTTGTGCA GTCTGTGAGT
    AACTTACTGA TGGAGGAAAA CAGGGACAGG TGGGAGGAAG CACAACTGCT GGGACCCAAT
    GTTAAGGAGC TTTTTCGTCT AGTAGAGGAC TTTGTGGACG TCATCAGCCT TCGCATGAAG
    GACTTCCAGG ATACTTATGA GGTCACGGAT AATCTAGTGT TGAGCATTCA CAAGCGGCCC
    GTGGCTGGAA ATGCAGATAT CACTTTCCCT ATGAAGGGCT GGAGGGGGAT GGTGGACTGG
    GCTCGCAGCT CTGAGGACAA AGTCAGAGTG GCCAAAAACA TCCTGCTCAC TGGAAAACCG
    GAAGAAGATG ACGCTTCAGC TTTTGTGACT GGGATCGTCC TGTACAGAAA CTTAGGCAGT
    ATTCTCTCTC TGCAGAGAAA TAGCACAGTG CTGAACTCCA AGGTGATCTC TGTGACAATC
    AAGCCCACTC CCAGCTCTCT CTCGAGTCCA CTAGAGATTG AGTTCTCACA CCTTTACAAT
    GGAACCACAA ACCAAACCTG CATTTCCTGG GATGAGAACG ACAGCTCGTC GTTGCTGGGC
    TCCTGGTCTG CGCGAGGCTG TAGAGCCGTG CCCGTCGACT CCAGCACAAC CAAATGCTTG
    TGTGACAGAC TCTCCACCTT CGCCATTTTA GCACGCCTCA ATCCCGATGT GAACATGGAT
    AAGACCCAGC TTCCATCTGT GACTCTGATC GTGGGCTGCG GGGTCTCGTC CCTCACCCTG
    CTCCTCCTCA TCATCATCTA TGTTTCTGTT TGGAGGTACA TCCGCTCAGA GCGCTCTGTC
    ATCCTCATCA ACTTCTGTCT GTCCATCATC TCCTCCAATG CGCTTATACT CATCGGTCAA
    ACTCAGACAC GGAACAAGGT TGTTTGTACT CTCGTGGCTG CGTTTCTGCA CTTTTTCTTC
    CTGTCCTCTT TCTGTTGGGT TCTAACGGAG GCATGGCAGT CGTACATGGC TGTGACCGGC
    CGCCTTAGAA ACCGTATCAT ACGCAAGCGT TTCCTTTGCC TTGGTTGGGG TTTACCTGCT
    CTCGTTGTAG CAATTTCTGT TGGATTCACC AAGGCAAAGG GATATGGCAC TGTTAACTAT
    TGCTGGCTGT CTCTGGAAGG AGGGCTGTTG TACGCATTTG TGGGACCAGC TGCTGCTGTC
    GTCCTGGTTA ACATGGTTAT CGGGATTCTG GTTTTTAACA AACTGGTGTC CAAAGATGGC
    ATTACTGATA TGAAACTCAA GGAGAGGGCC GGTCAGATGA CAGTGCCACT GTACAATATG
    ACGCTCAAAT GTGCCAAGTG CGGAGTTATC TCGTCGCCTG ACGTTTCCAC CACAGCTACC
    AGTAACGCCA TGGCGTCGCT GTGGAGCTCC TGCGTTGTTC TGCCGTTGCT GGCGCTCACT
    TGGATGTCGG CAGTCTTGGC TATTACTGAC CGCCGTTCAG CCTTGTTTCA AATCCTTTTT
    GCCGTCTTTG ACTCCCTCGA GGGCTTTGTT ATCGTGATGG TGCATTGCAT CCTACGCAGA
    GAGGTTCAAG AAGCAGTAAA GTGTCGTGTT GTTGACCGTC AAGAAGATGG AAACGGTGAC
    TCTGGTGGAT CCTTCCAGAA CGGTCATGCT CAGCTGATGA CTGACTTTGA AAAGGATGTG
    GACATAGCTT GTCGATCAGG CACCCTGAAG CGTCCCTCCC AGCAAGGCGA GGAGAAGGTT
    CCATCCCAGC AGATCACAGT ACAGAAAGGC TCCAACTTCA ACACCCTTCC TGCCAGCATG
    GCAAAGGTCC ACCTGCAAAA CGTAGCCAAC TACGCCAGTC ACACTCTCAC CATGCGCCGC
    GACAAAGGCG CCATCGCCGG GGTCAGCACG GAGTTACCCG GGGCCAAATC TATTTACATC
    TGTGACGGCG AGCTCTTCAA ACAGCTTGAC GGAGCACCGG GAGACATTCC TGAAGGAGGT
    GGTGGAGGTG CAGGTCAGGG TTATGTGCTT TTGCCCAACA ACAACAACAC CCTTAGGCCT
    ACGAAGGGTG GCAAGGAAGA CCCGGCCGCC AAATATAACA TCAGTATCGA ACAGTTACCC
    CAGACAAGGC TGATCCACCT GGCCAATCCT GTTGGCACCG ATCCCGTGGC AGGCTTCGGG
    CTGAAGAGCT TGCCCACCGA CCGAGTCAGC GTTTCATGCT CGGAGAGGGA GTCCCCGGTG
    CAGACGGTGC AGAATATCTC CAGCGAGTCC CAGATGACAA ATACGTGTGA GCAAGGGGAT
    TCTGGGAACT CTGGGATGTC TAAGAGCGAG ACTGTCTCCA CACTGTCCAT GAGCTCTCTG
    GAGAAGATCA TGCACACACG GAAGCGTCAT CAGGACATGT TTCAGGACCT GAATCGCAAA
    CTGCAGCACG CAGAGAAGGA CAGAGACTCT CCTCCAGTCG ACTGCAAGCC TGGAAAGAGA
    TGGAGCATTT CGTCAGGAGG AAGCGACAAA ACCAACCTTA GTGACAAGCA GCAGACCCCC
    AGTAAGCGGG CATGGGAGGG CATCAGGAAG ACTCACTCTC CTCCATCATG GGTGCGCAAG
    GAGCTGGAGC CGCTGCAGGC CTCACCGCTG GAGATGCAGG CAGTGGAGTG GGAGAAGGCC
    AGTGCCACCA TCCCTCTAGT GGGCCAGGAG ATCATGGACC TGCAGACGGA GGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290828.1
    CDS748..5544
    Translation

    Target ORF information:

    RefSeq Version XM_009292553.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292553.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCT ACAGGCAGTA TTCCTCAAAA
    GGCCAGGGTT TAAGTCCAGT GAAATACAAA GGAATGCAAG AGGCCATGCT TGGCAAAGGC
    CGGCTTGGCT CTCGTAGCCA ATCCCTGCGC TCGGTGGACA GCCGTAAACG AGAGGACTCT
    GATAAGACCC GCAGCGGACA GCAGTCTTCC CAAACAGTGG ATTCTCCGTC GGGTGAGGAG
    TGGTCTGCGT GGAGCGTGTG TTCGGCCACG TGTGGTGAGG GCTGGCAGAG CCGCACACGC
    TTCTGCGTCT CCGTGTCGTA CAGCACGCAG TGCAGCGGGC CACTGCGCGA ACAGAGACCC
    TGCAATAACA CTGCTGTGTG TCCAGCCGCT CTGATTACAG AACATGGGGC GTGGGGCGAG
    TGGACACCAT GGAGCTTGTG TTCATCAACA TGCGGCCGTG GCTACAGGGA ACGCAACCGC
    TCCTGCAAGC CACCCCAGTT CGGAGGAAAC CCCTGTGATG GTCCAGAGAA ACAGACTAAA
    TTCTGCAACA TCGCTGTATG CCCAGTGGAT GGCGTGTGGA ACGAGTGGTC TGGTTGGAGT
    TCCTGTTCTT CAACTTGTTC GAATGGAACA ATGCAGCGAA TTCGGGAGTG TAACGGGCCC
    TCGTATGGGG GCTTAGAGTG TCAAGGCGAG TGGCACGAGA CACGGGATTG CTTCCTTAGA
    GAATGTCCTG TGGATGGCCA ATGGCAACTT TGGAGCACTT GGTCTGGTTG CACCAAAACC
    TGTGGTGGAG GAAGTCAGCA GAGACAGAGG CTGTGTTATG GTCCCTTTTT TGGGGGAGAG
    GCATGCCCGG GGGACCGAGA AGAAGTTAGA CGTTGCAATG AGAAGCGATG CCCAGAGCCA
    CATGAAATCT GTGATGAGGA GAATTTCTCT AATGTAGTGT GGAAGAAAAC ACCTGCAGGG
    GACACAGCGG CTGTCCGCTG CCCACCAAAT GCTGTAGGAT TAATTCTACG GCGATGTTCA
    TTAGACAATG AGGGTATAGC ATACTGGGAG AACCCAACAT ATATGAAGTG CGTCTCAAAT
    GATTACAGAA GTATCCAAAC ACTGACCAGA GATCACCTGT CCAAGGCGCA GCGAGGGCTT
    GTGGGGGATG GTGTTTCTGA AGTGATGACA AAGCTGAGGG TGACGTCAAG TGATGGAACG
    AGCTACAGCG GAGATCTTTT GGCTATCATA GATGTGCTAA AAAACATGAC AGAGATATTT
    AGGAGATCCT ACTACAGCCC CAGTAATGCA GACATGAGGA ATTTTGTGCA GTCTGTGAGT
    AACTTACTGA TGGAGGAAAA CAGGGACAGG TGGGAGGAAG CACAACTGCT GGGACCCAAT
    GTTAAGGAGC TTTTTCGTCT AGTAGAGGAC TTTGTGGACG TCATCAGCCT TCGCATGAAG
    GACTTCCAGG ATACTTATGA GGTCACGGAT AATCTAGTGT TGAGCATTCA CAAGCGGCCC
    GTGGCTGGAA ATGCAGATAT CACTTTCCCT ATGAAGGGCT GGAGGGGGAT GGTGGACTGG
    GCTCGCAGCT CTGAGGACAA AGTCAGAGTG GCCAAAAACA TCCTGCTCAC TGGAAAACCG
    GAAGAAGATG ACGCTTCAGC TTTTGTGACT GGGATCGTCC TGTACAGAAA CTTAGGCAGT
    ATTCTCTCTC TGCAGAGAAA TAGCACAGTG CTGAACTCCA AGGTGATCTC TGTGACAATC
    AAGCCCACTC CCAGCTCTCT CTCGAGTCCA CTAGAGATTG AGTTCTCACA CCTTTACAAT
    GGAACCACAA ACCAAACCTG CATTTCCTGG GATGAGAACG ACAGCTCGTC GTTGCTGGGC
    TCCTGGTCTG CGCGAGGCTG TAGAGCCGTG CCCGTCGACT CCAGCACAAC CAAATGCTTG
    TGTGACAGAC TCTCCACCTT CGCCATTTTA GCACGCCTCA ATCCCGATGT GAACATGGAT
    AAGACCCAGC TTCCATCTGT GACTCTGATC GTGGGCTGCG GGGTCTCGTC CCTCACCCTG
    CTCCTCCTCA TCATCATCTA TGTTTCTGTT TGGAGGTACA TCCGCTCAGA GCGCTCTGTC
    ATCCTCATCA ACTTCTGTCT GTCCATCATC TCCTCCAATG CGCTTATACT CATCGGTCAA
    ACTCAGACAC GGAACAAGGT TGTTTGTACT CTCGTGGCTG CGTTTCTGCA CTTTTTCTTC
    CTGTCCTCTT TCTGTTGGGT TCTAACGGAG GCATGGCAGT CGTACATGGC TGTGACCGGC
    CGCCTTAGAA ACCGTATCAT ACGCAAGCGT TTCCTTTGCC TTGGTTGGGG TTTACCTGCT
    CTCGTTGTAG CAATTTCTGT TGGATTCACC AAGGCAAAGG GATATGGCAC TGTTAACTAT
    TGCTGGCTGT CTCTGGAAGG AGGGCTGTTG TACGCATTTG TGGGACCAGC TGCTGCTGTC
    GTCCTGGTTA ACATGGTTAT CGGGATTCTG GTTTTTAACA AACTGGTGTC CAAAGATGGC
    ATTACTGATA TGAAACTCAA GGAGAGGGCC GGTCAGATGA CAGTGCCACT GTACAATATG
    ACGCTCAAAT GTGCCAAGTG CGGAGTTATC TCGTCGCCTG ACGTTTCCAC CACAGCTACC
    AGTAACGCCA TGGCGTCGCT GTGGAGCTCC TGCGTTGTTC TGCCGTTGCT GGCGCTCACT
    TGGATGTCGG CAGTCTTGGC TATTACTGAC CGCCGTTCAG CCTTGTTTCA AATCCTTTTT
    GCCGTCTTTG ACTCCCTCGA GGGCTTTGTT ATCGTGATGG TGCATTGCAT CCTACGCAGA
    GAGGTTCAAG AAGCAGTAAA GTGTCGTGTT GTTGACCGTC AAGAAGATGG AAACGGTGAC
    TCTGGTGGAT CCTTCCAGAA CGGTCATGCT CAGCTGATGA CTGACTTTGA AAAGGATGTG
    GACATAGCTT GTCGATCAGG CACCCTGAAG CGTCCCTCCC AGCAAGGCGA GGAGAAGGTT
    CCATCCCAGC AGATCACAGT ACAGAAAGGC TCCAACTTCA ACACCCTTCC TGCCAGCATG
    GCAAAGGTCC ACCTGCAAAA CGTAGCCAAC TACGCCAGTC ACACTCTCAC CATGCGCCGC
    GACAAAGGCG CCATCGCCGG GGTCAGCACG GAGTTACCCG GGGCCAAATC TATTTACATC
    TGTGACGGCG AGCTCTTCAA ACAGCTTGAC GGAGCACCGG GAGACATTCC TGAAGGAGGT
    GGTGGAGGTG CAGGTCAGGG TTATGTGCTT TTGCCCAACA ACAACAACAC CCTTAGGCCT
    ACGAAGGGTG GCAAGGAAGA CCCGGCCGCC AAATATAACA TCAGTATCGA ACAGTTACCC
    CAGACAAGGC TGATCCACCT GGCCAATCCT GTTGGCACCG ATCCCGTGGC AGGCTTCGGG
    CTGAAGAGCT TGCCCACCGA CCGAGTCAGC GTTTCATGCT CGGAGAGGGA GTCCCCGGTG
    CAGACGGTGC AGAATATCTC CAGCGAGTCC CAGATGACAA ATACGTGTGA GCAAGGGGAT
    TCTGGGAACT CTGGGATGTC TAAGAGCGAG ACTGTCTCCA CACTGTCCAT GAGCTCTCTG
    GAGAAGATCA TGCACACACG GAAGCGTCAT CAGGACATGT TTCAGGACCT GAATCGCAAA
    CTGCAGCACG CAGAGAAGGA CAGAGACTCT CCTCCAGTCG ACTGCAAGCC TGGAAAGAGA
    TGGAGCATTT CGTCAGGAGG AAGCGACAAA ACCAACCTTA GTGACAAGCA GCAGACCCCC
    AGTAAGCGGG CATGGGAGGG CATCAGGAAG ACTCACTCTC CTCCATCATG GGTGCGCAAG
    GAGCTGGAGC CGCTGCAGGC CTCACCGCTG GAGATGCAGG CAGTGGAGTG GGAGAAGGCC
    AGTGCCACCA TCCCTCTAGT GGGCCAGGAG ATCATGGACC TGCAGACGGA GGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44097
    Clone ID Related Accession (Same CDS sequence) XM_009292554.2 , XM_009292554.3
    Accession Version XM_009292554.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4758bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product brain-specific angiogenesis inhibitor 1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292554.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCA AAGGCCGGCT TGGCTCTCGT
    AGCCAATCCC TGCGCTCGGT GGACAGCCGT AAACGAGAGG ACTCTGATAA GACCCGCAGC
    GGACAGCAGT CTTCCCAAAC AGTGGATTCT CCGTCGGGTG AGGAGTGGTC TGCGTGGAGC
    GTGTGTTCGG CCACGTGTGG TGAGGGCTGG CAGAGCCGCA CACGCTTCTG CGTCTCCGTG
    TCGTACAGCA CGCAGTGCAG CGGGCCACTG CGCGAACAGA GACCCTGCAA TAACACTGCT
    GTGTGTCCAG CCGCTCTGAT TACAGAACAT GGGGCGTGGG GCGAGTGGAC ACCATGGAGC
    TTGTGTTCAT CAACATGCGG CCGTGGCTAC AGGGAACGCA ACCGCTCCTG CAAGCCACCC
    CAGTTCGGAG GAAACCCCTG TGATGGTCCA GAGAAACAGA CTAAATTCTG CAACATCGCT
    GTATGCCCAG TGGATGGCGT GTGGAACGAG TGGTCTGGTT GGAGTTCCTG TTCTTCAACT
    TGTTCGAATG GAACAATGCA GCGAATTCGG GAGTGTAACG GGCCCTCGTA TGGGGGCTTA
    GAGTGTCAAG GCGAGTGGCA CGAGACACGG GATTGCTTCC TTAGAGAATG TCCTGTGGAT
    GGCCAATGGC AACTTTGGAG CACTTGGTCT GGTTGCACCA AAACCTGTGG TGGAGGAAGT
    CAGCAGAGAC AGAGGCTGTG TTATGGTCCC TTTTTTGGGG GAGAGGCATG CCCGGGGGAC
    CGAGAAGAAG TTAGACGTTG CAATGAGAAG CGATGCCCAG AGCCACATGA AATCTGTGAT
    GAGGAGAATT TCTCTAATGT AGTGTGGAAG AAAACACCTG CAGGGGACAC AGCGGCTGTC
    CGCTGCCCAC CAAATGCTGT AGGATTAATT CTACGGCGAT GTTCATTAGA CAATGAGGGT
    ATAGCATACT GGGAGAACCC AACATATATG AAGTGCGTCT CAAATGATTA CAGAAGTATC
    CAAACACTGA CCAGAGATCA CCTGTCCAAG GCGCAGCGAG GGCTTGTGGG GGATGGTGTT
    TCTGAAGTGA TGACAAAGCT GAGGGTGACG TCAAGTGATG GAACGAGCTA CAGCGGAGAT
    CTTTTGGCTA TCATAGATGT GCTAAAAAAC ATGACAGAGA TATTTAGGAG ATCCTACTAC
    AGCCCCAGTA ATGCAGACAT GAGGAATTTT GTGCAGTCTG TGAGTAACTT ACTGATGGAG
    GAAAACAGGG ACAGGTGGGA GGAAGCACAA CTGCTGGGAC CCAATGTTAA GGAGCTTTTT
    CGTCTAGTAG AGGACTTTGT GGACGTCATC AGCCTTCGCA TGAAGGACTT CCAGGATACT
    TATGAGGTCA CGGATAATCT AGTGTTGAGC ATTCACAAGC GGCCCGTGGC TGGAAATGCA
    GATATCACTT TCCCTATGAA GGGCTGGAGG GGGATGGTGG ACTGGGCTCG CAGCTCTGAG
    GACAAAGTCA GAGTGGCCAA AAACATCCTG CTCACTGGAA AACCGGAAGA AGATGACGCT
    TCAGCTTTTG TGACTGGGAT CGTCCTGTAC AGAAACTTAG GCAGTATTCT CTCTCTGCAG
    AGAAATAGCA CAGTGCTGAA CTCCAAGGTG ATCTCTGTGA CAATCAAGCC CACTCCCAGC
    TCTCTCTCGA GTCCACTAGA GATTGAGTTC TCACACCTTT ACAATGGAAC CACAAACCAA
    ACCTGCATTT CCTGGGATGA GAACGACAGC TCGTCGTTGC TGGGCTCCTG GTCTGCGCGA
    GGCTGTAGAG CCGTGCCCGT CGACTCCAGC ACAACCAAAT GCTTGTGTGA CAGACTCTCC
    ACCTTCGCCA TTTTAGCACG CCTCAATCCC GATGTGAACA TGGATAAGAC CCAGCTTCCA
    TCTGTGACTC TGATCGTGGG CTGCGGGGTC TCGTCCCTCA CCCTGCTCCT CCTCATCATC
    ATCTATGTTT CTGTTTGGAG GTACATCCGC TCAGAGCGCT CTGTCATCCT CATCAACTTC
    TGTCTGTCCA TCATCTCCTC CAATGCGCTT ATACTCATCG GTCAAACTCA GACACGGAAC
    AAGGTTGTTT GTACTCTCGT GGCTGCGTTT CTGCACTTTT TCTTCCTGTC CTCTTTCTGT
    TGGGTTCTAA CGGAGGCATG GCAGTCGTAC ATGGCTGTGA CCGGCCGCCT TAGAAACCGT
    ATCATACGCA AGCGTTTCCT TTGCCTTGGT TGGGGTTTAC CTGCTCTCGT TGTAGCAATT
    TCTGTTGGAT TCACCAAGGC AAAGGGATAT GGCACTGTTA ACTATTGCTG GCTGTCTCTG
    GAAGGAGGGC TGTTGTACGC ATTTGTGGGA CCAGCTGCTG CTGTCGTCCT GGTTAACATG
    GTTATCGGGA TTCTGGTTTT TAACAAACTG GTGTCCAAAG ATGGCATTAC TGATATGAAA
    CTCAAGGAGA GGGCCGGTCA GATGACAGTG CCACTGTACA ATATGACGCT CAAATGTGCC
    AAGTGCGGAG TTATCTCGTC GCCTGACGTT TCCACCACAG CTACCAGTAA CGCCATGGCG
    TCGCTGTGGA GCTCCTGCGT TGTTCTGCCG TTGCTGGCGC TCACTTGGAT GTCGGCAGTC
    TTGGCTATTA CTGACCGCCG TTCAGCCTTG TTTCAAATCC TTTTTGCCGT CTTTGACTCC
    CTCGAGGGCT TTGTTATCGT GATGGTGCAT TGCATCCTAC GCAGAGAGGT TCAAGAAGCA
    GTAAAGTGTC GTGTTGTTGA CCGTCAAGAA GATGGAAACG GTGACTCTGG TGGATCCTTC
    CAGAACGGTC ATGCTCAGCT GATGACTGAC TTTGAAAAGG ATGTGGACAT AGCTTGTCGA
    TCAGGCACCC TGAAGCGTCC CTCCCAGCAA GGCGAGGAGA AGGTTCCATC CCAGCAGATC
    ACAGTACAGA AAGGCTCCAA CTTCAACACC CTTCCTGCCA GCATGGCAAA GGTCCACCTG
    CAAAACGTAG CCAACTACGC CAGTCACACT CTCACCATGC GCCGCGACAA AGGCGCCATC
    GCCGGGGTCA GCACGGAGTT ACCCGGGGCC AAATCTATTT ACATCTGTGA CGGCGAGCTC
    TTCAAACAGC TTGACGGAGC ACCGGGAGAC ATTCCTGAAG GAGGTGGTGG AGGTGCAGGT
    CAGGGTTATG TGCTTTTGCC CAACAACAAC AACACCCTTA GGCCTACGAA GGGTGGCAAG
    GAAGACCCGG CCGCCAAATA TAACATCAGT ATCGAACAGT TACCCCAGAC AAGGCTGATC
    CACCTGGCCA ATCCTGTTGG CACCGATCCC GTGGCAGGCT TCGGGCTGAA GAGCTTGCCC
    ACCGACCGAG TCAGCGTTTC ATGCTCGGAG AGGGAGTCCC CGGTGCAGAC GGTGCAGAAT
    ATCTCCAGCG AGTCCCAGAT GACAAATACG TGTGAGCAAG GGGATTCTGG GAACTCTGGG
    ATGTCTAAGA GCGAGACTGT CTCCACACTG TCCATGAGCT CTCTGGAGAG ACGAAAATCA
    CGCTATGCAG AGCTGGACTT TGAGAAGATC ATGCACACAC GGAAGCGTCA TCAGGACATG
    TTTCAGGACC TGAATCGCAA ACTGCAGCAC GCAGAGAAGG ACAGAGACTC TCCTCCAGTC
    GACTGCAAGC CTGGAAAGAG ATGGAGCATT TCGTCAGGAG GAAGCGACAA AACCAACCTT
    AGTGACAAGC AGCAGACCCC CAGTAAGCGG GCATGGGAGG GCATCAGGAA GACTCACTCT
    CCTCCATCAT GGGTGCGCAA GGAGCTGGAG CCGCTGCAGG CCTCACCGCT GGAGATGCAG
    GCAGTGGAGT GGGAGAAGGC CAGTGCCACC ATCCCTCTAG TGGGCCAGGA GATCATGGAC
    CTGCAGACGG AGGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290829.1
    CDS731..5488
    Translation

    Target ORF information:

    RefSeq Version XM_009292554.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292554.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCA AAGGCCGGCT TGGCTCTCGT
    AGCCAATCCC TGCGCTCGGT GGACAGCCGT AAACGAGAGG ACTCTGATAA GACCCGCAGC
    GGACAGCAGT CTTCCCAAAC AGTGGATTCT CCGTCGGGTG AGGAGTGGTC TGCGTGGAGC
    GTGTGTTCGG CCACGTGTGG TGAGGGCTGG CAGAGCCGCA CACGCTTCTG CGTCTCCGTG
    TCGTACAGCA CGCAGTGCAG CGGGCCACTG CGCGAACAGA GACCCTGCAA TAACACTGCT
    GTGTGTCCAG CCGCTCTGAT TACAGAACAT GGGGCGTGGG GCGAGTGGAC ACCATGGAGC
    TTGTGTTCAT CAACATGCGG CCGTGGCTAC AGGGAACGCA ACCGCTCCTG CAAGCCACCC
    CAGTTCGGAG GAAACCCCTG TGATGGTCCA GAGAAACAGA CTAAATTCTG CAACATCGCT
    GTATGCCCAG TGGATGGCGT GTGGAACGAG TGGTCTGGTT GGAGTTCCTG TTCTTCAACT
    TGTTCGAATG GAACAATGCA GCGAATTCGG GAGTGTAACG GGCCCTCGTA TGGGGGCTTA
    GAGTGTCAAG GCGAGTGGCA CGAGACACGG GATTGCTTCC TTAGAGAATG TCCTGTGGAT
    GGCCAATGGC AACTTTGGAG CACTTGGTCT GGTTGCACCA AAACCTGTGG TGGAGGAAGT
    CAGCAGAGAC AGAGGCTGTG TTATGGTCCC TTTTTTGGGG GAGAGGCATG CCCGGGGGAC
    CGAGAAGAAG TTAGACGTTG CAATGAGAAG CGATGCCCAG AGCCACATGA AATCTGTGAT
    GAGGAGAATT TCTCTAATGT AGTGTGGAAG AAAACACCTG CAGGGGACAC AGCGGCTGTC
    CGCTGCCCAC CAAATGCTGT AGGATTAATT CTACGGCGAT GTTCATTAGA CAATGAGGGT
    ATAGCATACT GGGAGAACCC AACATATATG AAGTGCGTCT CAAATGATTA CAGAAGTATC
    CAAACACTGA CCAGAGATCA CCTGTCCAAG GCGCAGCGAG GGCTTGTGGG GGATGGTGTT
    TCTGAAGTGA TGACAAAGCT GAGGGTGACG TCAAGTGATG GAACGAGCTA CAGCGGAGAT
    CTTTTGGCTA TCATAGATGT GCTAAAAAAC ATGACAGAGA TATTTAGGAG ATCCTACTAC
    AGCCCCAGTA ATGCAGACAT GAGGAATTTT GTGCAGTCTG TGAGTAACTT ACTGATGGAG
    GAAAACAGGG ACAGGTGGGA GGAAGCACAA CTGCTGGGAC CCAATGTTAA GGAGCTTTTT
    CGTCTAGTAG AGGACTTTGT GGACGTCATC AGCCTTCGCA TGAAGGACTT CCAGGATACT
    TATGAGGTCA CGGATAATCT AGTGTTGAGC ATTCACAAGC GGCCCGTGGC TGGAAATGCA
    GATATCACTT TCCCTATGAA GGGCTGGAGG GGGATGGTGG ACTGGGCTCG CAGCTCTGAG
    GACAAAGTCA GAGTGGCCAA AAACATCCTG CTCACTGGAA AACCGGAAGA AGATGACGCT
    TCAGCTTTTG TGACTGGGAT CGTCCTGTAC AGAAACTTAG GCAGTATTCT CTCTCTGCAG
    AGAAATAGCA CAGTGCTGAA CTCCAAGGTG ATCTCTGTGA CAATCAAGCC CACTCCCAGC
    TCTCTCTCGA GTCCACTAGA GATTGAGTTC TCACACCTTT ACAATGGAAC CACAAACCAA
    ACCTGCATTT CCTGGGATGA GAACGACAGC TCGTCGTTGC TGGGCTCCTG GTCTGCGCGA
    GGCTGTAGAG CCGTGCCCGT CGACTCCAGC ACAACCAAAT GCTTGTGTGA CAGACTCTCC
    ACCTTCGCCA TTTTAGCACG CCTCAATCCC GATGTGAACA TGGATAAGAC CCAGCTTCCA
    TCTGTGACTC TGATCGTGGG CTGCGGGGTC TCGTCCCTCA CCCTGCTCCT CCTCATCATC
    ATCTATGTTT CTGTTTGGAG GTACATCCGC TCAGAGCGCT CTGTCATCCT CATCAACTTC
    TGTCTGTCCA TCATCTCCTC CAATGCGCTT ATACTCATCG GTCAAACTCA GACACGGAAC
    AAGGTTGTTT GTACTCTCGT GGCTGCGTTT CTGCACTTTT TCTTCCTGTC CTCTTTCTGT
    TGGGTTCTAA CGGAGGCATG GCAGTCGTAC ATGGCTGTGA CCGGCCGCCT TAGAAACCGT
    ATCATACGCA AGCGTTTCCT TTGCCTTGGT TGGGGTTTAC CTGCTCTCGT TGTAGCAATT
    TCTGTTGGAT TCACCAAGGC AAAGGGATAT GGCACTGTTA ACTATTGCTG GCTGTCTCTG
    GAAGGAGGGC TGTTGTACGC ATTTGTGGGA CCAGCTGCTG CTGTCGTCCT GGTTAACATG
    GTTATCGGGA TTCTGGTTTT TAACAAACTG GTGTCCAAAG ATGGCATTAC TGATATGAAA
    CTCAAGGAGA GGGCCGGTCA GATGACAGTG CCACTGTACA ATATGACGCT CAAATGTGCC
    AAGTGCGGAG TTATCTCGTC GCCTGACGTT TCCACCACAG CTACCAGTAA CGCCATGGCG
    TCGCTGTGGA GCTCCTGCGT TGTTCTGCCG TTGCTGGCGC TCACTTGGAT GTCGGCAGTC
    TTGGCTATTA CTGACCGCCG TTCAGCCTTG TTTCAAATCC TTTTTGCCGT CTTTGACTCC
    CTCGAGGGCT TTGTTATCGT GATGGTGCAT TGCATCCTAC GCAGAGAGGT TCAAGAAGCA
    GTAAAGTGTC GTGTTGTTGA CCGTCAAGAA GATGGAAACG GTGACTCTGG TGGATCCTTC
    CAGAACGGTC ATGCTCAGCT GATGACTGAC TTTGAAAAGG ATGTGGACAT AGCTTGTCGA
    TCAGGCACCC TGAAGCGTCC CTCCCAGCAA GGCGAGGAGA AGGTTCCATC CCAGCAGATC
    ACAGTACAGA AAGGCTCCAA CTTCAACACC CTTCCTGCCA GCATGGCAAA GGTCCACCTG
    CAAAACGTAG CCAACTACGC CAGTCACACT CTCACCATGC GCCGCGACAA AGGCGCCATC
    GCCGGGGTCA GCACGGAGTT ACCCGGGGCC AAATCTATTT ACATCTGTGA CGGCGAGCTC
    TTCAAACAGC TTGACGGAGC ACCGGGAGAC ATTCCTGAAG GAGGTGGTGG AGGTGCAGGT
    CAGGGTTATG TGCTTTTGCC CAACAACAAC AACACCCTTA GGCCTACGAA GGGTGGCAAG
    GAAGACCCGG CCGCCAAATA TAACATCAGT ATCGAACAGT TACCCCAGAC AAGGCTGATC
    CACCTGGCCA ATCCTGTTGG CACCGATCCC GTGGCAGGCT TCGGGCTGAA GAGCTTGCCC
    ACCGACCGAG TCAGCGTTTC ATGCTCGGAG AGGGAGTCCC CGGTGCAGAC GGTGCAGAAT
    ATCTCCAGCG AGTCCCAGAT GACAAATACG TGTGAGCAAG GGGATTCTGG GAACTCTGGG
    ATGTCTAAGA GCGAGACTGT CTCCACACTG TCCATGAGCT CTCTGGAGAG ACGAAAATCA
    CGCTATGCAG AGCTGGACTT TGAGAAGATC ATGCACACAC GGAAGCGTCA TCAGGACATG
    TTTCAGGACC TGAATCGCAA ACTGCAGCAC GCAGAGAAGG ACAGAGACTC TCCTCCAGTC
    GACTGCAAGC CTGGAAAGAG ATGGAGCATT TCGTCAGGAG GAAGCGACAA AACCAACCTT
    AGTGACAAGC AGCAGACCCC CAGTAAGCGG GCATGGGAGG GCATCAGGAA GACTCACTCT
    CCTCCATCAT GGGTGCGCAA GGAGCTGGAG CCGCTGCAGG CCTCACCGCT GGAGATGCAG
    GCAGTGGAGT GGGAGAAGGC CAGTGCCACC ATCCCTCTAG TGGGCCAGGA GATCATGGAC
    CTGCAGACGG AGGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44097
    Clone ID Related Accession (Same CDS sequence) XM_009292554.2 , XM_009292554.3
    Accession Version XM_009292554.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4758bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product brain-specific angiogenesis inhibitor 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009292554.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCA AAGGCCGGCT TGGCTCTCGT
    AGCCAATCCC TGCGCTCGGT GGACAGCCGT AAACGAGAGG ACTCTGATAA GACCCGCAGC
    GGACAGCAGT CTTCCCAAAC AGTGGATTCT CCGTCGGGTG AGGAGTGGTC TGCGTGGAGC
    GTGTGTTCGG CCACGTGTGG TGAGGGCTGG CAGAGCCGCA CACGCTTCTG CGTCTCCGTG
    TCGTACAGCA CGCAGTGCAG CGGGCCACTG CGCGAACAGA GACCCTGCAA TAACACTGCT
    GTGTGTCCAG CCGCTCTGAT TACAGAACAT GGGGCGTGGG GCGAGTGGAC ACCATGGAGC
    TTGTGTTCAT CAACATGCGG CCGTGGCTAC AGGGAACGCA ACCGCTCCTG CAAGCCACCC
    CAGTTCGGAG GAAACCCCTG TGATGGTCCA GAGAAACAGA CTAAATTCTG CAACATCGCT
    GTATGCCCAG TGGATGGCGT GTGGAACGAG TGGTCTGGTT GGAGTTCCTG TTCTTCAACT
    TGTTCGAATG GAACAATGCA GCGAATTCGG GAGTGTAACG GGCCCTCGTA TGGGGGCTTA
    GAGTGTCAAG GCGAGTGGCA CGAGACACGG GATTGCTTCC TTAGAGAATG TCCTGTGGAT
    GGCCAATGGC AACTTTGGAG CACTTGGTCT GGTTGCACCA AAACCTGTGG TGGAGGAAGT
    CAGCAGAGAC AGAGGCTGTG TTATGGTCCC TTTTTTGGGG GAGAGGCATG CCCGGGGGAC
    CGAGAAGAAG TTAGACGTTG CAATGAGAAG CGATGCCCAG AGCCACATGA AATCTGTGAT
    GAGGAGAATT TCTCTAATGT AGTGTGGAAG AAAACACCTG CAGGGGACAC AGCGGCTGTC
    CGCTGCCCAC CAAATGCTGT AGGATTAATT CTACGGCGAT GTTCATTAGA CAATGAGGGT
    ATAGCATACT GGGAGAACCC AACATATATG AAGTGCGTCT CAAATGATTA CAGAAGTATC
    CAAACACTGA CCAGAGATCA CCTGTCCAAG GCGCAGCGAG GGCTTGTGGG GGATGGTGTT
    TCTGAAGTGA TGACAAAGCT GAGGGTGACG TCAAGTGATG GAACGAGCTA CAGCGGAGAT
    CTTTTGGCTA TCATAGATGT GCTAAAAAAC ATGACAGAGA TATTTAGGAG ATCCTACTAC
    AGCCCCAGTA ATGCAGACAT GAGGAATTTT GTGCAGTCTG TGAGTAACTT ACTGATGGAG
    GAAAACAGGG ACAGGTGGGA GGAAGCACAA CTGCTGGGAC CCAATGTTAA GGAGCTTTTT
    CGTCTAGTAG AGGACTTTGT GGACGTCATC AGCCTTCGCA TGAAGGACTT CCAGGATACT
    TATGAGGTCA CGGATAATCT AGTGTTGAGC ATTCACAAGC GGCCCGTGGC TGGAAATGCA
    GATATCACTT TCCCTATGAA GGGCTGGAGG GGGATGGTGG ACTGGGCTCG CAGCTCTGAG
    GACAAAGTCA GAGTGGCCAA AAACATCCTG CTCACTGGAA AACCGGAAGA AGATGACGCT
    TCAGCTTTTG TGACTGGGAT CGTCCTGTAC AGAAACTTAG GCAGTATTCT CTCTCTGCAG
    AGAAATAGCA CAGTGCTGAA CTCCAAGGTG ATCTCTGTGA CAATCAAGCC CACTCCCAGC
    TCTCTCTCGA GTCCACTAGA GATTGAGTTC TCACACCTTT ACAATGGAAC CACAAACCAA
    ACCTGCATTT CCTGGGATGA GAACGACAGC TCGTCGTTGC TGGGCTCCTG GTCTGCGCGA
    GGCTGTAGAG CCGTGCCCGT CGACTCCAGC ACAACCAAAT GCTTGTGTGA CAGACTCTCC
    ACCTTCGCCA TTTTAGCACG CCTCAATCCC GATGTGAACA TGGATAAGAC CCAGCTTCCA
    TCTGTGACTC TGATCGTGGG CTGCGGGGTC TCGTCCCTCA CCCTGCTCCT CCTCATCATC
    ATCTATGTTT CTGTTTGGAG GTACATCCGC TCAGAGCGCT CTGTCATCCT CATCAACTTC
    TGTCTGTCCA TCATCTCCTC CAATGCGCTT ATACTCATCG GTCAAACTCA GACACGGAAC
    AAGGTTGTTT GTACTCTCGT GGCTGCGTTT CTGCACTTTT TCTTCCTGTC CTCTTTCTGT
    TGGGTTCTAA CGGAGGCATG GCAGTCGTAC ATGGCTGTGA CCGGCCGCCT TAGAAACCGT
    ATCATACGCA AGCGTTTCCT TTGCCTTGGT TGGGGTTTAC CTGCTCTCGT TGTAGCAATT
    TCTGTTGGAT TCACCAAGGC AAAGGGATAT GGCACTGTTA ACTATTGCTG GCTGTCTCTG
    GAAGGAGGGC TGTTGTACGC ATTTGTGGGA CCAGCTGCTG CTGTCGTCCT GGTTAACATG
    GTTATCGGGA TTCTGGTTTT TAACAAACTG GTGTCCAAAG ATGGCATTAC TGATATGAAA
    CTCAAGGAGA GGGCCGGTCA GATGACAGTG CCACTGTACA ATATGACGCT CAAATGTGCC
    AAGTGCGGAG TTATCTCGTC GCCTGACGTT TCCACCACAG CTACCAGTAA CGCCATGGCG
    TCGCTGTGGA GCTCCTGCGT TGTTCTGCCG TTGCTGGCGC TCACTTGGAT GTCGGCAGTC
    TTGGCTATTA CTGACCGCCG TTCAGCCTTG TTTCAAATCC TTTTTGCCGT CTTTGACTCC
    CTCGAGGGCT TTGTTATCGT GATGGTGCAT TGCATCCTAC GCAGAGAGGT TCAAGAAGCA
    GTAAAGTGTC GTGTTGTTGA CCGTCAAGAA GATGGAAACG GTGACTCTGG TGGATCCTTC
    CAGAACGGTC ATGCTCAGCT GATGACTGAC TTTGAAAAGG ATGTGGACAT AGCTTGTCGA
    TCAGGCACCC TGAAGCGTCC CTCCCAGCAA GGCGAGGAGA AGGTTCCATC CCAGCAGATC
    ACAGTACAGA AAGGCTCCAA CTTCAACACC CTTCCTGCCA GCATGGCAAA GGTCCACCTG
    CAAAACGTAG CCAACTACGC CAGTCACACT CTCACCATGC GCCGCGACAA AGGCGCCATC
    GCCGGGGTCA GCACGGAGTT ACCCGGGGCC AAATCTATTT ACATCTGTGA CGGCGAGCTC
    TTCAAACAGC TTGACGGAGC ACCGGGAGAC ATTCCTGAAG GAGGTGGTGG AGGTGCAGGT
    CAGGGTTATG TGCTTTTGCC CAACAACAAC AACACCCTTA GGCCTACGAA GGGTGGCAAG
    GAAGACCCGG CCGCCAAATA TAACATCAGT ATCGAACAGT TACCCCAGAC AAGGCTGATC
    CACCTGGCCA ATCCTGTTGG CACCGATCCC GTGGCAGGCT TCGGGCTGAA GAGCTTGCCC
    ACCGACCGAG TCAGCGTTTC ATGCTCGGAG AGGGAGTCCC CGGTGCAGAC GGTGCAGAAT
    ATCTCCAGCG AGTCCCAGAT GACAAATACG TGTGAGCAAG GGGATTCTGG GAACTCTGGG
    ATGTCTAAGA GCGAGACTGT CTCCACACTG TCCATGAGCT CTCTGGAGAG ACGAAAATCA
    CGCTATGCAG AGCTGGACTT TGAGAAGATC ATGCACACAC GGAAGCGTCA TCAGGACATG
    TTTCAGGACC TGAATCGCAA ACTGCAGCAC GCAGAGAAGG ACAGAGACTC TCCTCCAGTC
    GACTGCAAGC CTGGAAAGAG ATGGAGCATT TCGTCAGGAG GAAGCGACAA AACCAACCTT
    AGTGACAAGC AGCAGACCCC CAGTAAGCGG GCATGGGAGG GCATCAGGAA GACTCACTCT
    CCTCCATCAT GGGTGCGCAA GGAGCTGGAG CCGCTGCAGG CCTCACCGCT GGAGATGCAG
    GCAGTGGAGT GGGAGAAGGC CAGTGCCACC ATCCCTCTAG TGGGCCAGGA GATCATGGAC
    CTGCAGACGG AGGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290829.1
    CDS747..5504
    Translation

    Target ORF information:

    RefSeq Version XM_009292554.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292554.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCA AAGGCCGGCT TGGCTCTCGT
    AGCCAATCCC TGCGCTCGGT GGACAGCCGT AAACGAGAGG ACTCTGATAA GACCCGCAGC
    GGACAGCAGT CTTCCCAAAC AGTGGATTCT CCGTCGGGTG AGGAGTGGTC TGCGTGGAGC
    GTGTGTTCGG CCACGTGTGG TGAGGGCTGG CAGAGCCGCA CACGCTTCTG CGTCTCCGTG
    TCGTACAGCA CGCAGTGCAG CGGGCCACTG CGCGAACAGA GACCCTGCAA TAACACTGCT
    GTGTGTCCAG CCGCTCTGAT TACAGAACAT GGGGCGTGGG GCGAGTGGAC ACCATGGAGC
    TTGTGTTCAT CAACATGCGG CCGTGGCTAC AGGGAACGCA ACCGCTCCTG CAAGCCACCC
    CAGTTCGGAG GAAACCCCTG TGATGGTCCA GAGAAACAGA CTAAATTCTG CAACATCGCT
    GTATGCCCAG TGGATGGCGT GTGGAACGAG TGGTCTGGTT GGAGTTCCTG TTCTTCAACT
    TGTTCGAATG GAACAATGCA GCGAATTCGG GAGTGTAACG GGCCCTCGTA TGGGGGCTTA
    GAGTGTCAAG GCGAGTGGCA CGAGACACGG GATTGCTTCC TTAGAGAATG TCCTGTGGAT
    GGCCAATGGC AACTTTGGAG CACTTGGTCT GGTTGCACCA AAACCTGTGG TGGAGGAAGT
    CAGCAGAGAC AGAGGCTGTG TTATGGTCCC TTTTTTGGGG GAGAGGCATG CCCGGGGGAC
    CGAGAAGAAG TTAGACGTTG CAATGAGAAG CGATGCCCAG AGCCACATGA AATCTGTGAT
    GAGGAGAATT TCTCTAATGT AGTGTGGAAG AAAACACCTG CAGGGGACAC AGCGGCTGTC
    CGCTGCCCAC CAAATGCTGT AGGATTAATT CTACGGCGAT GTTCATTAGA CAATGAGGGT
    ATAGCATACT GGGAGAACCC AACATATATG AAGTGCGTCT CAAATGATTA CAGAAGTATC
    CAAACACTGA CCAGAGATCA CCTGTCCAAG GCGCAGCGAG GGCTTGTGGG GGATGGTGTT
    TCTGAAGTGA TGACAAAGCT GAGGGTGACG TCAAGTGATG GAACGAGCTA CAGCGGAGAT
    CTTTTGGCTA TCATAGATGT GCTAAAAAAC ATGACAGAGA TATTTAGGAG ATCCTACTAC
    AGCCCCAGTA ATGCAGACAT GAGGAATTTT GTGCAGTCTG TGAGTAACTT ACTGATGGAG
    GAAAACAGGG ACAGGTGGGA GGAAGCACAA CTGCTGGGAC CCAATGTTAA GGAGCTTTTT
    CGTCTAGTAG AGGACTTTGT GGACGTCATC AGCCTTCGCA TGAAGGACTT CCAGGATACT
    TATGAGGTCA CGGATAATCT AGTGTTGAGC ATTCACAAGC GGCCCGTGGC TGGAAATGCA
    GATATCACTT TCCCTATGAA GGGCTGGAGG GGGATGGTGG ACTGGGCTCG CAGCTCTGAG
    GACAAAGTCA GAGTGGCCAA AAACATCCTG CTCACTGGAA AACCGGAAGA AGATGACGCT
    TCAGCTTTTG TGACTGGGAT CGTCCTGTAC AGAAACTTAG GCAGTATTCT CTCTCTGCAG
    AGAAATAGCA CAGTGCTGAA CTCCAAGGTG ATCTCTGTGA CAATCAAGCC CACTCCCAGC
    TCTCTCTCGA GTCCACTAGA GATTGAGTTC TCACACCTTT ACAATGGAAC CACAAACCAA
    ACCTGCATTT CCTGGGATGA GAACGACAGC TCGTCGTTGC TGGGCTCCTG GTCTGCGCGA
    GGCTGTAGAG CCGTGCCCGT CGACTCCAGC ACAACCAAAT GCTTGTGTGA CAGACTCTCC
    ACCTTCGCCA TTTTAGCACG CCTCAATCCC GATGTGAACA TGGATAAGAC CCAGCTTCCA
    TCTGTGACTC TGATCGTGGG CTGCGGGGTC TCGTCCCTCA CCCTGCTCCT CCTCATCATC
    ATCTATGTTT CTGTTTGGAG GTACATCCGC TCAGAGCGCT CTGTCATCCT CATCAACTTC
    TGTCTGTCCA TCATCTCCTC CAATGCGCTT ATACTCATCG GTCAAACTCA GACACGGAAC
    AAGGTTGTTT GTACTCTCGT GGCTGCGTTT CTGCACTTTT TCTTCCTGTC CTCTTTCTGT
    TGGGTTCTAA CGGAGGCATG GCAGTCGTAC ATGGCTGTGA CCGGCCGCCT TAGAAACCGT
    ATCATACGCA AGCGTTTCCT TTGCCTTGGT TGGGGTTTAC CTGCTCTCGT TGTAGCAATT
    TCTGTTGGAT TCACCAAGGC AAAGGGATAT GGCACTGTTA ACTATTGCTG GCTGTCTCTG
    GAAGGAGGGC TGTTGTACGC ATTTGTGGGA CCAGCTGCTG CTGTCGTCCT GGTTAACATG
    GTTATCGGGA TTCTGGTTTT TAACAAACTG GTGTCCAAAG ATGGCATTAC TGATATGAAA
    CTCAAGGAGA GGGCCGGTCA GATGACAGTG CCACTGTACA ATATGACGCT CAAATGTGCC
    AAGTGCGGAG TTATCTCGTC GCCTGACGTT TCCACCACAG CTACCAGTAA CGCCATGGCG
    TCGCTGTGGA GCTCCTGCGT TGTTCTGCCG TTGCTGGCGC TCACTTGGAT GTCGGCAGTC
    TTGGCTATTA CTGACCGCCG TTCAGCCTTG TTTCAAATCC TTTTTGCCGT CTTTGACTCC
    CTCGAGGGCT TTGTTATCGT GATGGTGCAT TGCATCCTAC GCAGAGAGGT TCAAGAAGCA
    GTAAAGTGTC GTGTTGTTGA CCGTCAAGAA GATGGAAACG GTGACTCTGG TGGATCCTTC
    CAGAACGGTC ATGCTCAGCT GATGACTGAC TTTGAAAAGG ATGTGGACAT AGCTTGTCGA
    TCAGGCACCC TGAAGCGTCC CTCCCAGCAA GGCGAGGAGA AGGTTCCATC CCAGCAGATC
    ACAGTACAGA AAGGCTCCAA CTTCAACACC CTTCCTGCCA GCATGGCAAA GGTCCACCTG
    CAAAACGTAG CCAACTACGC CAGTCACACT CTCACCATGC GCCGCGACAA AGGCGCCATC
    GCCGGGGTCA GCACGGAGTT ACCCGGGGCC AAATCTATTT ACATCTGTGA CGGCGAGCTC
    TTCAAACAGC TTGACGGAGC ACCGGGAGAC ATTCCTGAAG GAGGTGGTGG AGGTGCAGGT
    CAGGGTTATG TGCTTTTGCC CAACAACAAC AACACCCTTA GGCCTACGAA GGGTGGCAAG
    GAAGACCCGG CCGCCAAATA TAACATCAGT ATCGAACAGT TACCCCAGAC AAGGCTGATC
    CACCTGGCCA ATCCTGTTGG CACCGATCCC GTGGCAGGCT TCGGGCTGAA GAGCTTGCCC
    ACCGACCGAG TCAGCGTTTC ATGCTCGGAG AGGGAGTCCC CGGTGCAGAC GGTGCAGAAT
    ATCTCCAGCG AGTCCCAGAT GACAAATACG TGTGAGCAAG GGGATTCTGG GAACTCTGGG
    ATGTCTAAGA GCGAGACTGT CTCCACACTG TCCATGAGCT CTCTGGAGAG ACGAAAATCA
    CGCTATGCAG AGCTGGACTT TGAGAAGATC ATGCACACAC GGAAGCGTCA TCAGGACATG
    TTTCAGGACC TGAATCGCAA ACTGCAGCAC GCAGAGAAGG ACAGAGACTC TCCTCCAGTC
    GACTGCAAGC CTGGAAAGAG ATGGAGCATT TCGTCAGGAG GAAGCGACAA AACCAACCTT
    AGTGACAAGC AGCAGACCCC CAGTAAGCGG GCATGGGAGG GCATCAGGAA GACTCACTCT
    CCTCCATCAT GGGTGCGCAA GGAGCTGGAG CCGCTGCAGG CCTCACCGCT GGAGATGCAG
    GCAGTGGAGT GGGAGAAGGC CAGTGCCACC ATCCCTCTAG TGGGCCAGGA GATCATGGAC
    CTGCAGACGG AGGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44098
    Clone ID Related Accession (Same CDS sequence) XM_009292555.3 , XM_009292555.2
    Accession Version XM_009292555.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product brain-specific angiogenesis inhibitor 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009292555.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCA AAGGCCGGCT TGGCTCTCGT
    AGCCAATCCC TGCGCTCGGT GGACAGCCGT AAACGAGAGG ACTCTGATAA GACCCGCAGC
    GGACAGCAGT CTTCCCAAAC AGTGGATTCT CCGTCGGGTG AGGAGTGGTC TGCGTGGAGC
    GTGTGTTCGG CCACGTGTGG TGAGGGCTGG CAGAGCCGCA CACGCTTCTG CGTCTCCGTG
    TCGTACAGCA CGCAGTGCAG CGGGCCACTG CGCGAACAGA GACCCTGCAA TAACACTGCT
    GTGTGTCCAG AACATGGGGC GTGGGGCGAG TGGACACCAT GGAGCTTGTG TTCATCAACA
    TGCGGCCGTG GCTACAGGGA ACGCAACCGC TCCTGCAAGC CACCCCAGTT CGGAGGAAAC
    CCCTGTGATG GTCCAGAGAA ACAGACTAAA TTCTGCAACA TCGCTGTATG CCCAGTGGAT
    GGCGTGTGGA ACGAGTGGTC TGGTTGGAGT TCCTGTTCTT CAACTTGTTC GAATGGAACA
    ATGCAGCGAA TTCGGGAGTG TAACGGGCCC TCGTATGGGG GCTTAGAGTG TCAAGGCGAG
    TGGCACGAGA CACGGGATTG CTTCCTTAGA GAATGTCCTG TGGATGGCCA ATGGCAACTT
    TGGAGCACTT GGTCTGGTTG CACCAAAACC TGTGGTGGAG GAAGTCAGCA GAGACAGAGG
    CTGTGTTATG GTCCCTTTTT TGGGGGAGAG GCATGCCCGG GGGACCGAGA AGAAGTTAGA
    CGTTGCAATG AGAAGCGATG CCCAGAGCCA CATGAAATCT GTGATGAGGA GAATTTCTCT
    AATGTAGTGT GGAAGAAAAC ACCTGCAGGG GACACAGCGG CTGTCCGCTG CCCACCAAAT
    GCTGTAGGAT TAATTCTACG GCGATGTTCA TTAGACAATG AGGGTATAGC ATACTGGGAG
    AACCCAACAT ATATGAAGTG CGTCTCAAAT GATTACAGAA GTATCCAAAC ACTGACCAGA
    GATCACCTGT CCAAGGCGCA GCGAGGGCTT GTGGGGGATG GTGTTTCTGA AGTGATGACA
    AAGCTGAGGG TGACGTCAAG TGATGGAACG AGCTACAGCG GAGATCTTTT GGCTATCATA
    GATGTGCTAA AAAACATGAC AGAGATATTT AGGAGATCCT ACTACAGCCC CAGTAATGCA
    GACATGAGGA ATTTTGTGCA GTCTGTGAGT AACTTACTGA TGGAGGAAAA CAGGGACAGG
    TGGGAGGAAG CACAACTGCT GGGACCCAAT GTTAAGGAGC TTTTTCGTCT AGTAGAGGAC
    TTTGTGGACG TCATCAGCCT TCGCATGAAG GACTTCCAGG ATACTTATGA GGTCACGGAT
    AATCTAGTGT TGAGCATTCA CAAGCGGCCC GTGGCTGGAA ATGCAGATAT CACTTTCCCT
    ATGAAGGGCT GGAGGGGGAT GGTGGACTGG GCTCGCAGCT CTGAGGACAA AGTCAGAGTG
    GCCAAAAACA TCCTGCTCAC TGGAAAACCG GAAGAAGATG ACGCTTCAGC TTTTGTGACT
    GGGATCGTCC TGTACAGAAA CTTAGGCAGT ATTCTCTCTC TGCAGAGAAA TAGCACAGTG
    CTGAACTCCA AGGTGATCTC TGTGACAATC AAGCCCACTC CCAGCTCTCT CTCGAGTCCA
    CTAGAGATTG AGTTCTCACA CCTTTACAAT GGAACCACAA ACCAAACCTG CATTTCCTGG
    GATGAGAACG ACAGCTCGTC GTTGCTGGGC TCCTGGTCTG CGCGAGGCTG TAGAGCCGTG
    CCCGTCGACT CCAGCACAAC CAAATGCTTG TGTGACAGAC TCTCCACCTT CGCCATTTTA
    GCACGCCTCA ATCCCGATGT GAACATGGAT AAGACCCAGC TTCCATCTGT GACTCTGATC
    GTGGGCTGCG GGGTCTCGTC CCTCACCCTG CTCCTCCTCA TCATCATCTA TGTTTCTGTT
    TGGAGGTACA TCCGCTCAGA GCGCTCTGTC ATCCTCATCA ACTTCTGTCT GTCCATCATC
    TCCTCCAATG CGCTTATACT CATCGGTCAA ACTCAGACAC GGAACAAGGT TGTTTGTACT
    CTCGTGGCTG CGTTTCTGCA CTTTTTCTTC CTGTCCTCTT TCTGTTGGGT TCTAACGGAG
    GCATGGCAGT CGTACATGGC TGTGACCGGC CGCCTTAGAA ACCGTATCAT ACGCAAGCGT
    TTCCTTTGCC TTGGTTGGGG TTTACCTGCT CTCGTTGTAG CAATTTCTGT TGGATTCACC
    AAGGCAAAGG GATATGGCAC TGTTAACTAT TGCTGGCTGT CTCTGGAAGG AGGGCTGTTG
    TACGCATTTG TGGGACCAGC TGCTGCTGTC GTCCTGGTTA ACATGGTTAT CGGGATTCTG
    GTTTTTAACA AACTGGTGTC CAAAGATGGC ATTACTGATA TGAAACTCAA GGAGAGGGCC
    GGTCAGATGA CAGTGCCACT GTACAATATG ACGCTCAAAT GTGCCAAGTG CGGAGTTATC
    TCGTCGCCTG ACGTTTCCAC CACAGCTACC AGTAACGCCA TGGCGTCGCT GTGGAGCTCC
    TGCGTTGTTC TGCCGTTGCT GGCGCTCACT TGGATGTCGG CAGTCTTGGC TATTACTGAC
    CGCCGTTCAG CCTTGTTTCA AATCCTTTTT GCCGTCTTTG ACTCCCTCGA GGGCTTTGTT
    ATCGTGATGG TGCATTGCAT CCTACGCAGA GAGGTTCAAG AAGCAGTAAA GTGTCGTGTT
    GTTGACCGTC AAGAAGATGG AAACGGTGAC TCTGGTGGAT CCTTCCAGAA CGGTCATGCT
    CAGCTGATGA CTGACTTTGA AAAGGATGTG GACATAGCTT GTCGATCAGG CACCCTGAAG
    CGTCCCTCCC AGCAAGGCGA GGAGAAGGTT CCATCCCAGC AGATCACAGT ACAGAAAGGC
    TCCAACTTCA ACACCCTTCC TGCCAGCATG GCAAAGGTCC ACCTGCAAAA CGTAGCCAAC
    TACGCCAGTC ACACTCTCAC CATGCGCCGC GACAAAGGCG CCATCGCCGG GGTCAGCACG
    GAGTTACCCG GGGCCAAATC TATTTACATC TGTGACGGCG AGCTCTTCAA ACAGCTTGAC
    GGAGCACCGG GAGACATTCC TGAAGGAGGT GGTGGAGGTG CAGGTCAGGG TTATGTGCTT
    TTGCCCAACA ACAACAACAC CCTTAGGCCT ACGAAGGGTG GCAAGGAAGA CCCGGCCGCC
    AAATATAACA TCAGTATCGA ACAGTTACCC CAGACAAGGC TGATCCACCT GGCCAATCCT
    GTTGGCACCG ATCCCGTGGC AGGCTTCGGG CTGAAGAGCT TGCCCACCGA CCGAGTCAGC
    GTTTCATGCT CGGAGAGGGA GTCCCCGGTG CAGACGGTGC AGAATATCTC CAGCGAGTCC
    CAGATGACAA ATACGTGTGA GCAAGGGGAT TCTGGGAACT CTGGGATGTC TAAGAGCGAG
    ACTGTCTCCA CACTGTCCAT GAGCTCTCTG GAGAGACGAA AATCACGCTA TGCAGAGCTG
    GACTTTGAGA AGATCATGCA CACACGGAAG CGTCATCAGG ACATGTTTCA GGACCTGAAT
    CGCAAACTGC AGCACGCAGA GAAGGACAGA GACTCTCCTC CAGTCGACTG CAAGCCTGGA
    AAGAGATGGA GCATTTCGTC AGGAGGAAGC GACAAAACCA ACCTTAGTGA CAAGCAGCAG
    ACCCCCAGTA AGCGGGCATG GGAGGGCATC AGGAAGACTC ACTCTCCTCC ATCATGGGTG
    CGCAAGGAGC TGGAGCCGCT GCAGGCCTCA CCGCTGGAGA TGCAGGCAGT GGAGTGGGAG
    AAGGCCAGTG CCACCATCCC TCTAGTGGGC CAGGAGATCA TGGACCTGCA GACGGAGGTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290830.1
    CDS747..5489
    Translation

    Target ORF information:

    RefSeq Version XM_009292555.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292555.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCA AAGGCCGGCT TGGCTCTCGT
    AGCCAATCCC TGCGCTCGGT GGACAGCCGT AAACGAGAGG ACTCTGATAA GACCCGCAGC
    GGACAGCAGT CTTCCCAAAC AGTGGATTCT CCGTCGGGTG AGGAGTGGTC TGCGTGGAGC
    GTGTGTTCGG CCACGTGTGG TGAGGGCTGG CAGAGCCGCA CACGCTTCTG CGTCTCCGTG
    TCGTACAGCA CGCAGTGCAG CGGGCCACTG CGCGAACAGA GACCCTGCAA TAACACTGCT
    GTGTGTCCAG AACATGGGGC GTGGGGCGAG TGGACACCAT GGAGCTTGTG TTCATCAACA
    TGCGGCCGTG GCTACAGGGA ACGCAACCGC TCCTGCAAGC CACCCCAGTT CGGAGGAAAC
    CCCTGTGATG GTCCAGAGAA ACAGACTAAA TTCTGCAACA TCGCTGTATG CCCAGTGGAT
    GGCGTGTGGA ACGAGTGGTC TGGTTGGAGT TCCTGTTCTT CAACTTGTTC GAATGGAACA
    ATGCAGCGAA TTCGGGAGTG TAACGGGCCC TCGTATGGGG GCTTAGAGTG TCAAGGCGAG
    TGGCACGAGA CACGGGATTG CTTCCTTAGA GAATGTCCTG TGGATGGCCA ATGGCAACTT
    TGGAGCACTT GGTCTGGTTG CACCAAAACC TGTGGTGGAG GAAGTCAGCA GAGACAGAGG
    CTGTGTTATG GTCCCTTTTT TGGGGGAGAG GCATGCCCGG GGGACCGAGA AGAAGTTAGA
    CGTTGCAATG AGAAGCGATG CCCAGAGCCA CATGAAATCT GTGATGAGGA GAATTTCTCT
    AATGTAGTGT GGAAGAAAAC ACCTGCAGGG GACACAGCGG CTGTCCGCTG CCCACCAAAT
    GCTGTAGGAT TAATTCTACG GCGATGTTCA TTAGACAATG AGGGTATAGC ATACTGGGAG
    AACCCAACAT ATATGAAGTG CGTCTCAAAT GATTACAGAA GTATCCAAAC ACTGACCAGA
    GATCACCTGT CCAAGGCGCA GCGAGGGCTT GTGGGGGATG GTGTTTCTGA AGTGATGACA
    AAGCTGAGGG TGACGTCAAG TGATGGAACG AGCTACAGCG GAGATCTTTT GGCTATCATA
    GATGTGCTAA AAAACATGAC AGAGATATTT AGGAGATCCT ACTACAGCCC CAGTAATGCA
    GACATGAGGA ATTTTGTGCA GTCTGTGAGT AACTTACTGA TGGAGGAAAA CAGGGACAGG
    TGGGAGGAAG CACAACTGCT GGGACCCAAT GTTAAGGAGC TTTTTCGTCT AGTAGAGGAC
    TTTGTGGACG TCATCAGCCT TCGCATGAAG GACTTCCAGG ATACTTATGA GGTCACGGAT
    AATCTAGTGT TGAGCATTCA CAAGCGGCCC GTGGCTGGAA ATGCAGATAT CACTTTCCCT
    ATGAAGGGCT GGAGGGGGAT GGTGGACTGG GCTCGCAGCT CTGAGGACAA AGTCAGAGTG
    GCCAAAAACA TCCTGCTCAC TGGAAAACCG GAAGAAGATG ACGCTTCAGC TTTTGTGACT
    GGGATCGTCC TGTACAGAAA CTTAGGCAGT ATTCTCTCTC TGCAGAGAAA TAGCACAGTG
    CTGAACTCCA AGGTGATCTC TGTGACAATC AAGCCCACTC CCAGCTCTCT CTCGAGTCCA
    CTAGAGATTG AGTTCTCACA CCTTTACAAT GGAACCACAA ACCAAACCTG CATTTCCTGG
    GATGAGAACG ACAGCTCGTC GTTGCTGGGC TCCTGGTCTG CGCGAGGCTG TAGAGCCGTG
    CCCGTCGACT CCAGCACAAC CAAATGCTTG TGTGACAGAC TCTCCACCTT CGCCATTTTA
    GCACGCCTCA ATCCCGATGT GAACATGGAT AAGACCCAGC TTCCATCTGT GACTCTGATC
    GTGGGCTGCG GGGTCTCGTC CCTCACCCTG CTCCTCCTCA TCATCATCTA TGTTTCTGTT
    TGGAGGTACA TCCGCTCAGA GCGCTCTGTC ATCCTCATCA ACTTCTGTCT GTCCATCATC
    TCCTCCAATG CGCTTATACT CATCGGTCAA ACTCAGACAC GGAACAAGGT TGTTTGTACT
    CTCGTGGCTG CGTTTCTGCA CTTTTTCTTC CTGTCCTCTT TCTGTTGGGT TCTAACGGAG
    GCATGGCAGT CGTACATGGC TGTGACCGGC CGCCTTAGAA ACCGTATCAT ACGCAAGCGT
    TTCCTTTGCC TTGGTTGGGG TTTACCTGCT CTCGTTGTAG CAATTTCTGT TGGATTCACC
    AAGGCAAAGG GATATGGCAC TGTTAACTAT TGCTGGCTGT CTCTGGAAGG AGGGCTGTTG
    TACGCATTTG TGGGACCAGC TGCTGCTGTC GTCCTGGTTA ACATGGTTAT CGGGATTCTG
    GTTTTTAACA AACTGGTGTC CAAAGATGGC ATTACTGATA TGAAACTCAA GGAGAGGGCC
    GGTCAGATGA CAGTGCCACT GTACAATATG ACGCTCAAAT GTGCCAAGTG CGGAGTTATC
    TCGTCGCCTG ACGTTTCCAC CACAGCTACC AGTAACGCCA TGGCGTCGCT GTGGAGCTCC
    TGCGTTGTTC TGCCGTTGCT GGCGCTCACT TGGATGTCGG CAGTCTTGGC TATTACTGAC
    CGCCGTTCAG CCTTGTTTCA AATCCTTTTT GCCGTCTTTG ACTCCCTCGA GGGCTTTGTT
    ATCGTGATGG TGCATTGCAT CCTACGCAGA GAGGTTCAAG AAGCAGTAAA GTGTCGTGTT
    GTTGACCGTC AAGAAGATGG AAACGGTGAC TCTGGTGGAT CCTTCCAGAA CGGTCATGCT
    CAGCTGATGA CTGACTTTGA AAAGGATGTG GACATAGCTT GTCGATCAGG CACCCTGAAG
    CGTCCCTCCC AGCAAGGCGA GGAGAAGGTT CCATCCCAGC AGATCACAGT ACAGAAAGGC
    TCCAACTTCA ACACCCTTCC TGCCAGCATG GCAAAGGTCC ACCTGCAAAA CGTAGCCAAC
    TACGCCAGTC ACACTCTCAC CATGCGCCGC GACAAAGGCG CCATCGCCGG GGTCAGCACG
    GAGTTACCCG GGGCCAAATC TATTTACATC TGTGACGGCG AGCTCTTCAA ACAGCTTGAC
    GGAGCACCGG GAGACATTCC TGAAGGAGGT GGTGGAGGTG CAGGTCAGGG TTATGTGCTT
    TTGCCCAACA ACAACAACAC CCTTAGGCCT ACGAAGGGTG GCAAGGAAGA CCCGGCCGCC
    AAATATAACA TCAGTATCGA ACAGTTACCC CAGACAAGGC TGATCCACCT GGCCAATCCT
    GTTGGCACCG ATCCCGTGGC AGGCTTCGGG CTGAAGAGCT TGCCCACCGA CCGAGTCAGC
    GTTTCATGCT CGGAGAGGGA GTCCCCGGTG CAGACGGTGC AGAATATCTC CAGCGAGTCC
    CAGATGACAA ATACGTGTGA GCAAGGGGAT TCTGGGAACT CTGGGATGTC TAAGAGCGAG
    ACTGTCTCCA CACTGTCCAT GAGCTCTCTG GAGAGACGAA AATCACGCTA TGCAGAGCTG
    GACTTTGAGA AGATCATGCA CACACGGAAG CGTCATCAGG ACATGTTTCA GGACCTGAAT
    CGCAAACTGC AGCACGCAGA GAAGGACAGA GACTCTCCTC CAGTCGACTG CAAGCCTGGA
    AAGAGATGGA GCATTTCGTC AGGAGGAAGC GACAAAACCA ACCTTAGTGA CAAGCAGCAG
    ACCCCCAGTA AGCGGGCATG GGAGGGCATC AGGAAGACTC ACTCTCCTCC ATCATGGGTG
    CGCAAGGAGC TGGAGCCGCT GCAGGCCTCA CCGCTGGAGA TGCAGGCAGT GGAGTGGGAG
    AAGGCCAGTG CCACCATCCC TCTAGTGGGC CAGGAGATCA TGGACCTGCA GACGGAGGTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44098
    Clone ID Related Accession (Same CDS sequence) XM_009292555.3 , XM_009292555.2
    Accession Version XM_009292555.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product brain-specific angiogenesis inhibitor 1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292555.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCA AAGGCCGGCT TGGCTCTCGT
    AGCCAATCCC TGCGCTCGGT GGACAGCCGT AAACGAGAGG ACTCTGATAA GACCCGCAGC
    GGACAGCAGT CTTCCCAAAC AGTGGATTCT CCGTCGGGTG AGGAGTGGTC TGCGTGGAGC
    GTGTGTTCGG CCACGTGTGG TGAGGGCTGG CAGAGCCGCA CACGCTTCTG CGTCTCCGTG
    TCGTACAGCA CGCAGTGCAG CGGGCCACTG CGCGAACAGA GACCCTGCAA TAACACTGCT
    GTGTGTCCAG AACATGGGGC GTGGGGCGAG TGGACACCAT GGAGCTTGTG TTCATCAACA
    TGCGGCCGTG GCTACAGGGA ACGCAACCGC TCCTGCAAGC CACCCCAGTT CGGAGGAAAC
    CCCTGTGATG GTCCAGAGAA ACAGACTAAA TTCTGCAACA TCGCTGTATG CCCAGTGGAT
    GGCGTGTGGA ACGAGTGGTC TGGTTGGAGT TCCTGTTCTT CAACTTGTTC GAATGGAACA
    ATGCAGCGAA TTCGGGAGTG TAACGGGCCC TCGTATGGGG GCTTAGAGTG TCAAGGCGAG
    TGGCACGAGA CACGGGATTG CTTCCTTAGA GAATGTCCTG TGGATGGCCA ATGGCAACTT
    TGGAGCACTT GGTCTGGTTG CACCAAAACC TGTGGTGGAG GAAGTCAGCA GAGACAGAGG
    CTGTGTTATG GTCCCTTTTT TGGGGGAGAG GCATGCCCGG GGGACCGAGA AGAAGTTAGA
    CGTTGCAATG AGAAGCGATG CCCAGAGCCA CATGAAATCT GTGATGAGGA GAATTTCTCT
    AATGTAGTGT GGAAGAAAAC ACCTGCAGGG GACACAGCGG CTGTCCGCTG CCCACCAAAT
    GCTGTAGGAT TAATTCTACG GCGATGTTCA TTAGACAATG AGGGTATAGC ATACTGGGAG
    AACCCAACAT ATATGAAGTG CGTCTCAAAT GATTACAGAA GTATCCAAAC ACTGACCAGA
    GATCACCTGT CCAAGGCGCA GCGAGGGCTT GTGGGGGATG GTGTTTCTGA AGTGATGACA
    AAGCTGAGGG TGACGTCAAG TGATGGAACG AGCTACAGCG GAGATCTTTT GGCTATCATA
    GATGTGCTAA AAAACATGAC AGAGATATTT AGGAGATCCT ACTACAGCCC CAGTAATGCA
    GACATGAGGA ATTTTGTGCA GTCTGTGAGT AACTTACTGA TGGAGGAAAA CAGGGACAGG
    TGGGAGGAAG CACAACTGCT GGGACCCAAT GTTAAGGAGC TTTTTCGTCT AGTAGAGGAC
    TTTGTGGACG TCATCAGCCT TCGCATGAAG GACTTCCAGG ATACTTATGA GGTCACGGAT
    AATCTAGTGT TGAGCATTCA CAAGCGGCCC GTGGCTGGAA ATGCAGATAT CACTTTCCCT
    ATGAAGGGCT GGAGGGGGAT GGTGGACTGG GCTCGCAGCT CTGAGGACAA AGTCAGAGTG
    GCCAAAAACA TCCTGCTCAC TGGAAAACCG GAAGAAGATG ACGCTTCAGC TTTTGTGACT
    GGGATCGTCC TGTACAGAAA CTTAGGCAGT ATTCTCTCTC TGCAGAGAAA TAGCACAGTG
    CTGAACTCCA AGGTGATCTC TGTGACAATC AAGCCCACTC CCAGCTCTCT CTCGAGTCCA
    CTAGAGATTG AGTTCTCACA CCTTTACAAT GGAACCACAA ACCAAACCTG CATTTCCTGG
    GATGAGAACG ACAGCTCGTC GTTGCTGGGC TCCTGGTCTG CGCGAGGCTG TAGAGCCGTG
    CCCGTCGACT CCAGCACAAC CAAATGCTTG TGTGACAGAC TCTCCACCTT CGCCATTTTA
    GCACGCCTCA ATCCCGATGT GAACATGGAT AAGACCCAGC TTCCATCTGT GACTCTGATC
    GTGGGCTGCG GGGTCTCGTC CCTCACCCTG CTCCTCCTCA TCATCATCTA TGTTTCTGTT
    TGGAGGTACA TCCGCTCAGA GCGCTCTGTC ATCCTCATCA ACTTCTGTCT GTCCATCATC
    TCCTCCAATG CGCTTATACT CATCGGTCAA ACTCAGACAC GGAACAAGGT TGTTTGTACT
    CTCGTGGCTG CGTTTCTGCA CTTTTTCTTC CTGTCCTCTT TCTGTTGGGT TCTAACGGAG
    GCATGGCAGT CGTACATGGC TGTGACCGGC CGCCTTAGAA ACCGTATCAT ACGCAAGCGT
    TTCCTTTGCC TTGGTTGGGG TTTACCTGCT CTCGTTGTAG CAATTTCTGT TGGATTCACC
    AAGGCAAAGG GATATGGCAC TGTTAACTAT TGCTGGCTGT CTCTGGAAGG AGGGCTGTTG
    TACGCATTTG TGGGACCAGC TGCTGCTGTC GTCCTGGTTA ACATGGTTAT CGGGATTCTG
    GTTTTTAACA AACTGGTGTC CAAAGATGGC ATTACTGATA TGAAACTCAA GGAGAGGGCC
    GGTCAGATGA CAGTGCCACT GTACAATATG ACGCTCAAAT GTGCCAAGTG CGGAGTTATC
    TCGTCGCCTG ACGTTTCCAC CACAGCTACC AGTAACGCCA TGGCGTCGCT GTGGAGCTCC
    TGCGTTGTTC TGCCGTTGCT GGCGCTCACT TGGATGTCGG CAGTCTTGGC TATTACTGAC
    CGCCGTTCAG CCTTGTTTCA AATCCTTTTT GCCGTCTTTG ACTCCCTCGA GGGCTTTGTT
    ATCGTGATGG TGCATTGCAT CCTACGCAGA GAGGTTCAAG AAGCAGTAAA GTGTCGTGTT
    GTTGACCGTC AAGAAGATGG AAACGGTGAC TCTGGTGGAT CCTTCCAGAA CGGTCATGCT
    CAGCTGATGA CTGACTTTGA AAAGGATGTG GACATAGCTT GTCGATCAGG CACCCTGAAG
    CGTCCCTCCC AGCAAGGCGA GGAGAAGGTT CCATCCCAGC AGATCACAGT ACAGAAAGGC
    TCCAACTTCA ACACCCTTCC TGCCAGCATG GCAAAGGTCC ACCTGCAAAA CGTAGCCAAC
    TACGCCAGTC ACACTCTCAC CATGCGCCGC GACAAAGGCG CCATCGCCGG GGTCAGCACG
    GAGTTACCCG GGGCCAAATC TATTTACATC TGTGACGGCG AGCTCTTCAA ACAGCTTGAC
    GGAGCACCGG GAGACATTCC TGAAGGAGGT GGTGGAGGTG CAGGTCAGGG TTATGTGCTT
    TTGCCCAACA ACAACAACAC CCTTAGGCCT ACGAAGGGTG GCAAGGAAGA CCCGGCCGCC
    AAATATAACA TCAGTATCGA ACAGTTACCC CAGACAAGGC TGATCCACCT GGCCAATCCT
    GTTGGCACCG ATCCCGTGGC AGGCTTCGGG CTGAAGAGCT TGCCCACCGA CCGAGTCAGC
    GTTTCATGCT CGGAGAGGGA GTCCCCGGTG CAGACGGTGC AGAATATCTC CAGCGAGTCC
    CAGATGACAA ATACGTGTGA GCAAGGGGAT TCTGGGAACT CTGGGATGTC TAAGAGCGAG
    ACTGTCTCCA CACTGTCCAT GAGCTCTCTG GAGAGACGAA AATCACGCTA TGCAGAGCTG
    GACTTTGAGA AGATCATGCA CACACGGAAG CGTCATCAGG ACATGTTTCA GGACCTGAAT
    CGCAAACTGC AGCACGCAGA GAAGGACAGA GACTCTCCTC CAGTCGACTG CAAGCCTGGA
    AAGAGATGGA GCATTTCGTC AGGAGGAAGC GACAAAACCA ACCTTAGTGA CAAGCAGCAG
    ACCCCCAGTA AGCGGGCATG GGAGGGCATC AGGAAGACTC ACTCTCCTCC ATCATGGGTG
    CGCAAGGAGC TGGAGCCGCT GCAGGCCTCA CCGCTGGAGA TGCAGGCAGT GGAGTGGGAG
    AAGGCCAGTG CCACCATCCC TCTAGTGGGC CAGGAGATCA TGGACCTGCA GACGGAGGTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290830.1
    CDS731..5473
    Translation

    Target ORF information:

    RefSeq Version XM_009292555.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292555.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCA AAGGCCGGCT TGGCTCTCGT
    AGCCAATCCC TGCGCTCGGT GGACAGCCGT AAACGAGAGG ACTCTGATAA GACCCGCAGC
    GGACAGCAGT CTTCCCAAAC AGTGGATTCT CCGTCGGGTG AGGAGTGGTC TGCGTGGAGC
    GTGTGTTCGG CCACGTGTGG TGAGGGCTGG CAGAGCCGCA CACGCTTCTG CGTCTCCGTG
    TCGTACAGCA CGCAGTGCAG CGGGCCACTG CGCGAACAGA GACCCTGCAA TAACACTGCT
    GTGTGTCCAG AACATGGGGC GTGGGGCGAG TGGACACCAT GGAGCTTGTG TTCATCAACA
    TGCGGCCGTG GCTACAGGGA ACGCAACCGC TCCTGCAAGC CACCCCAGTT CGGAGGAAAC
    CCCTGTGATG GTCCAGAGAA ACAGACTAAA TTCTGCAACA TCGCTGTATG CCCAGTGGAT
    GGCGTGTGGA ACGAGTGGTC TGGTTGGAGT TCCTGTTCTT CAACTTGTTC GAATGGAACA
    ATGCAGCGAA TTCGGGAGTG TAACGGGCCC TCGTATGGGG GCTTAGAGTG TCAAGGCGAG
    TGGCACGAGA CACGGGATTG CTTCCTTAGA GAATGTCCTG TGGATGGCCA ATGGCAACTT
    TGGAGCACTT GGTCTGGTTG CACCAAAACC TGTGGTGGAG GAAGTCAGCA GAGACAGAGG
    CTGTGTTATG GTCCCTTTTT TGGGGGAGAG GCATGCCCGG GGGACCGAGA AGAAGTTAGA
    CGTTGCAATG AGAAGCGATG CCCAGAGCCA CATGAAATCT GTGATGAGGA GAATTTCTCT
    AATGTAGTGT GGAAGAAAAC ACCTGCAGGG GACACAGCGG CTGTCCGCTG CCCACCAAAT
    GCTGTAGGAT TAATTCTACG GCGATGTTCA TTAGACAATG AGGGTATAGC ATACTGGGAG
    AACCCAACAT ATATGAAGTG CGTCTCAAAT GATTACAGAA GTATCCAAAC ACTGACCAGA
    GATCACCTGT CCAAGGCGCA GCGAGGGCTT GTGGGGGATG GTGTTTCTGA AGTGATGACA
    AAGCTGAGGG TGACGTCAAG TGATGGAACG AGCTACAGCG GAGATCTTTT GGCTATCATA
    GATGTGCTAA AAAACATGAC AGAGATATTT AGGAGATCCT ACTACAGCCC CAGTAATGCA
    GACATGAGGA ATTTTGTGCA GTCTGTGAGT AACTTACTGA TGGAGGAAAA CAGGGACAGG
    TGGGAGGAAG CACAACTGCT GGGACCCAAT GTTAAGGAGC TTTTTCGTCT AGTAGAGGAC
    TTTGTGGACG TCATCAGCCT TCGCATGAAG GACTTCCAGG ATACTTATGA GGTCACGGAT
    AATCTAGTGT TGAGCATTCA CAAGCGGCCC GTGGCTGGAA ATGCAGATAT CACTTTCCCT
    ATGAAGGGCT GGAGGGGGAT GGTGGACTGG GCTCGCAGCT CTGAGGACAA AGTCAGAGTG
    GCCAAAAACA TCCTGCTCAC TGGAAAACCG GAAGAAGATG ACGCTTCAGC TTTTGTGACT
    GGGATCGTCC TGTACAGAAA CTTAGGCAGT ATTCTCTCTC TGCAGAGAAA TAGCACAGTG
    CTGAACTCCA AGGTGATCTC TGTGACAATC AAGCCCACTC CCAGCTCTCT CTCGAGTCCA
    CTAGAGATTG AGTTCTCACA CCTTTACAAT GGAACCACAA ACCAAACCTG CATTTCCTGG
    GATGAGAACG ACAGCTCGTC GTTGCTGGGC TCCTGGTCTG CGCGAGGCTG TAGAGCCGTG
    CCCGTCGACT CCAGCACAAC CAAATGCTTG TGTGACAGAC TCTCCACCTT CGCCATTTTA
    GCACGCCTCA ATCCCGATGT GAACATGGAT AAGACCCAGC TTCCATCTGT GACTCTGATC
    GTGGGCTGCG GGGTCTCGTC CCTCACCCTG CTCCTCCTCA TCATCATCTA TGTTTCTGTT
    TGGAGGTACA TCCGCTCAGA GCGCTCTGTC ATCCTCATCA ACTTCTGTCT GTCCATCATC
    TCCTCCAATG CGCTTATACT CATCGGTCAA ACTCAGACAC GGAACAAGGT TGTTTGTACT
    CTCGTGGCTG CGTTTCTGCA CTTTTTCTTC CTGTCCTCTT TCTGTTGGGT TCTAACGGAG
    GCATGGCAGT CGTACATGGC TGTGACCGGC CGCCTTAGAA ACCGTATCAT ACGCAAGCGT
    TTCCTTTGCC TTGGTTGGGG TTTACCTGCT CTCGTTGTAG CAATTTCTGT TGGATTCACC
    AAGGCAAAGG GATATGGCAC TGTTAACTAT TGCTGGCTGT CTCTGGAAGG AGGGCTGTTG
    TACGCATTTG TGGGACCAGC TGCTGCTGTC GTCCTGGTTA ACATGGTTAT CGGGATTCTG
    GTTTTTAACA AACTGGTGTC CAAAGATGGC ATTACTGATA TGAAACTCAA GGAGAGGGCC
    GGTCAGATGA CAGTGCCACT GTACAATATG ACGCTCAAAT GTGCCAAGTG CGGAGTTATC
    TCGTCGCCTG ACGTTTCCAC CACAGCTACC AGTAACGCCA TGGCGTCGCT GTGGAGCTCC
    TGCGTTGTTC TGCCGTTGCT GGCGCTCACT TGGATGTCGG CAGTCTTGGC TATTACTGAC
    CGCCGTTCAG CCTTGTTTCA AATCCTTTTT GCCGTCTTTG ACTCCCTCGA GGGCTTTGTT
    ATCGTGATGG TGCATTGCAT CCTACGCAGA GAGGTTCAAG AAGCAGTAAA GTGTCGTGTT
    GTTGACCGTC AAGAAGATGG AAACGGTGAC TCTGGTGGAT CCTTCCAGAA CGGTCATGCT
    CAGCTGATGA CTGACTTTGA AAAGGATGTG GACATAGCTT GTCGATCAGG CACCCTGAAG
    CGTCCCTCCC AGCAAGGCGA GGAGAAGGTT CCATCCCAGC AGATCACAGT ACAGAAAGGC
    TCCAACTTCA ACACCCTTCC TGCCAGCATG GCAAAGGTCC ACCTGCAAAA CGTAGCCAAC
    TACGCCAGTC ACACTCTCAC CATGCGCCGC GACAAAGGCG CCATCGCCGG GGTCAGCACG
    GAGTTACCCG GGGCCAAATC TATTTACATC TGTGACGGCG AGCTCTTCAA ACAGCTTGAC
    GGAGCACCGG GAGACATTCC TGAAGGAGGT GGTGGAGGTG CAGGTCAGGG TTATGTGCTT
    TTGCCCAACA ACAACAACAC CCTTAGGCCT ACGAAGGGTG GCAAGGAAGA CCCGGCCGCC
    AAATATAACA TCAGTATCGA ACAGTTACCC CAGACAAGGC TGATCCACCT GGCCAATCCT
    GTTGGCACCG ATCCCGTGGC AGGCTTCGGG CTGAAGAGCT TGCCCACCGA CCGAGTCAGC
    GTTTCATGCT CGGAGAGGGA GTCCCCGGTG CAGACGGTGC AGAATATCTC CAGCGAGTCC
    CAGATGACAA ATACGTGTGA GCAAGGGGAT TCTGGGAACT CTGGGATGTC TAAGAGCGAG
    ACTGTCTCCA CACTGTCCAT GAGCTCTCTG GAGAGACGAA AATCACGCTA TGCAGAGCTG
    GACTTTGAGA AGATCATGCA CACACGGAAG CGTCATCAGG ACATGTTTCA GGACCTGAAT
    CGCAAACTGC AGCACGCAGA GAAGGACAGA GACTCTCCTC CAGTCGACTG CAAGCCTGGA
    AAGAGATGGA GCATTTCGTC AGGAGGAAGC GACAAAACCA ACCTTAGTGA CAAGCAGCAG
    ACCCCCAGTA AGCGGGCATG GGAGGGCATC AGGAAGACTC ACTCTCCTCC ATCATGGGTG
    CGCAAGGAGC TGGAGCCGCT GCAGGCCTCA CCGCTGGAGA TGCAGGCAGT GGAGTGGGAG
    AAGGCCAGTG CCACCATCCC TCTAGTGGGC CAGGAGATCA TGGACCTGCA GACGGAGGTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44099
    Clone ID Related Accession (Same CDS sequence) XM_009292556.2 , XM_009292556.3
    Accession Version XM_009292556.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4734bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product brain-specific angiogenesis inhibitor 1 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292556.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCT ACAGGCAGTA TTCCTCAAAA
    GGCCAGGGTT TAAGTCCAGT GAAATACAAA GGAATGCAAG AGGCCATGCT TGGCAAAGGC
    CGGCTTGGCT CTCGTAGCCA ATCCCTGCGC TCGGTGGACA GCCGTAAACG AGAGGACTCT
    GATAAGACCC GCAGCGGACA GCAGTCTTCC CAAACAGTGG ATTCTCCGTC GGGTGAGGAG
    TGGTCTGCGT GGAGCGTGTG TTCGGCCACG TGTGGTGAGG GCTGGCAGAG CCGCACACGC
    TTCTGCGTCT CCGTGTCGTA CAGCACGCAG TGCAGCGGGC CACTGCGCGA ACAGAGACCC
    TGCAATAACA CTGCTGTGTG TCCAGCCGCT CTGATTACAG AACATGGGGC GTGGGGCGAG
    TGGACACCAT GGAGCTTGTG TTCATCAACA TGCGGCCGTG GCTACAGGGA ACGCAACCGC
    TCCTGCAAGC CACCCCAGTT CGGAGGAAAC CCCTGTGATG GTCCAGAGAA ACAGACTAAA
    TTCTGCAACA TCGCTGTATG CCCAGTGGAT GGCGTGTGGA ACGAGTGGTC TGGTTGGAGT
    TCCTGTTCTT CAACTTGTTC GAATGGAACA ATGCAGCGAA TTCGGGAGTG TAACGGGCCC
    TCGTATGGGG GCTTAGAGTG TCAAGGCGAG TGGCACGAGA CACGGGATTG CTTCCTTAGA
    GAATGTCCTG TGGATGGCCA ATGGCAACTT TGGAGCACTT GGTCTGGTTG CACCAAAACC
    TGTGGTGGAG GAAGTCAGCA GAGACAGAGG CTGTGTTATG GTCCCTTTTT TGGGGGAGAG
    GCATGCCCGG GGGACCGAGA AGAAGTTAGA CGTTGCAATG AGAAGCGATG CCCAGAGCCA
    CATGAAATCT GTGATGAGGA GAATTTCTCT AATGTAGTGT GGAAGAAAAC ACCTGCAGGG
    GACACAGCGG CTGTCCGCTG CCCACCAAAT GCTGTAGGAT TAATTCTACG GCGATGTTCA
    TTAGACAATG AGGGTATAGC ATACTGGGAG AACCCAACAT ATATGAAGTG CGTCTCAAAT
    GATTACAGAA GTATCCAAAC ACTGACCAGA GATCACCTGT CCAAGGCGCA GCGAGGGCTT
    GTGGGGGATG GTGTTTCTGA AGTGATGACA AAGCTGAGGG TGACGTCAAG TGATGGAACG
    AGCTACAGCG GAGATCTTTT GGCTATCATA GATGTGCTAA AAAACATGAC AGAGATATTT
    AGGAGATCCT ACTACAGCCC CAGTAATGCA GACATGAGGA ATTTTGTGCA GTCTGTGAGT
    AACTTACTGA TGGAGGAAAA CAGGGACAGG TGGGAGGAAG CACAACTGCT GGGACCCAAT
    GTTAAGGAGC TTTTTCGTCT AGTAGAGGAC TTTGTGGACG TCATCAGCCT TCGCATGAAG
    GACTTCCAGG ATACTTATGA GGTCACGGAT AATCTAGTGT TGAGCATTCA CAAGCGGCCC
    GTGGCTGGAA ATGCAGATAT CACTTTCCCT ATGAAGGGCT GGAGGGGGAT GGTGGACTGG
    GCTCGCAGCT CTGAGGACAA AGTCAGAGTG GCCAAAAACA TCCTGCTCAC TGGAAAACCG
    GAAGAAGATG ACGCTTCAGC TTTTGTGACT GGGATCGTCC TGTACAGAAA CTTAGGCAGT
    ATTCTCTCTC TGCAGAGAAA TAGCACAGTG CTGAACTCCA AGGTGATCTC TGTGACAATC
    AAGCCCACTC CCAGCTCTCT CTCGAGTCCA CTAGAGATTG AGTTCTCACA CCTTTACAAT
    GGAACCACAA ACCAAACCTG CATTTCCTGG GATGAGAACG ACAGCTCGTC GTTGCTGGGC
    TCCTGGTCTG CGCGAGGCTG TAGAGCCGTG CCCGTCGACT CCAGCACAAC CAAATGCTTG
    TGTGACAGAC TCTCCACCTT CGCCATTTTA GCACGCCTCA ATCCCGATGT GAACATGGAT
    AAGACCCAGC TTCCATCTGT GACTCTGATC GTGGGCTGCG GGGTCTCGTC CCTCACCCTG
    CTCCTCCTCA TCATCATCTA TGTTTCTGTT TGGAGGTACA TCCGCTCAGA GCGCTCTGTC
    ATCCTCATCA ACTTCTGTCT GTCCATCATC TCCTCCAATG CGCTTATACT CATCGGTCAA
    ACTCAGACAC GGAACAAGGT TGTTTGTACT CTCGTGGCTG CGTTTCTGCA CTTTTTCTTC
    CTGTCCTCTT TCTGTTGGGT TCTAACGGAG GCATGGCAGT CGTACATGGC TGTGACCGGC
    CGCCTTAGAA ACCGTATCAT ACGCAAGCGT TTCCTTTGCC TTGGTTGGGG TTTACCTGCT
    CTCGTTGTAG CAATTTCTGT TGGATTCACC AAGGCAAAGG GATATGGCAC TGTTAACTAT
    TGCTGGCTGT CTCTGGAAGG AGGGCTGTTG TACGCATTTG TGGGACCAGC TGCTGCTGTC
    GTCCTGGTTA ACATGGTTAT CGGGATTCTG GTTTTTAACA AACTGGTGTC CAAAGATGGC
    ATTACTGATA TGAAACTCAA GGAGAGGGCC GGGGCGTCGC TGTGGAGCTC CTGCGTTGTT
    CTGCCGTTGC TGGCGCTCAC TTGGATGTCG GCAGTCTTGG CTATTACTGA CCGCCGTTCA
    GCCTTGTTTC AAATCCTTTT TGCCGTCTTT GACTCCCTCG AGGGCTTTGT TATCGTGATG
    GTGCATTGCA TCCTACGCAG AGAGGTTCAA GAAGCAGTAA AGTGTCGTGT TGTTGACCGT
    CAAGAAGATG GAAACGGTGA CTCTGGTGGA TCCTTCCAGA ACGGTCATGC TCAGCTGATG
    ACTGACTTTG AAAAGGATGT GGACATAGCT TGTCGATCAG GCACCCTGAA GCGTCCCTCC
    CAGCAAGGCG AGGAGAAGGT TCCATCCCAG CAGATCACAG TACAGAAAGG CTCCAACTTC
    AACACCCTTC CTGCCAGCAT GGCAAAGGTC CACCTGCAAA ACGTAGCCAA CTACGCCAGT
    CACACTCTCA CCATGCGCCG CGACAAAGGC GCCATCGCCG GGGTCAGCAC GGAGTTACCC
    GGGGCCAAAT CTATTTACAT CTGTGACGGC GAGCTCTTCA AACAGCTTGA CGGAGCACCG
    GGAGACATTC CTGAAGGAGG TGGTGGAGGT GCAGGTCAGG GTTATGTGCT TTTGCCCAAC
    AACAACAACA CCCTTAGGCC TACGAAGGGT GGCAAGGAAG ACCCGGCCGC CAAATATAAC
    ATCAGTATCG AACAGTTACC CCAGACAAGG CTGATCCACC TGGCCAATCC TGTTGGCACC
    GATCCCGTGG CAGGCTTCGG GCTGAAGAGC TTGCCCACCG ACCGAGTCAG CGTTTCATGC
    TCGGAGAGGG AGTCCCCGGT GCAGACGGTG CAGAATATCT CCAGCGAGTC CCAGATGACA
    AATACGTGTG AGCAAGGGGA TTCTGGGAAC TCTGGGATGT CTAAGAGCGA GACTGTCTCC
    ACACTGTCCA TGAGCTCTCT GGAGAGACGA AAATCACGCT ATGCAGAGCT GGACTTTGAG
    AAGATCATGC ACACACGGAA GCGTCATCAG GACATGTTTC AGGACCTGAA TCGCAAACTG
    CAGCACGCAG AGAAGGACAG AGACTCTCCT CCAGTCGACT GCAAGCCTGG AAAGAGATGG
    AGCATTTCGT CAGGAGGAAG CGACAAAACC AACCTTAGTG ACAAGCAGCA GACCCCCAGT
    AAGCGGGCAT GGGAGGGCAT CAGGAAGACT CACTCTCCTC CATCATGGGT GCGCAAGGAG
    CTGGAGCCGC TGCAGGCCTC ACCGCTGGAG ATGCAGGCAG TGGAGTGGGA GAAGGCCAGT
    GCCACCATCC CTCTAGTGGG CCAGGAGATC ATGGACCTGC AGACGGAGGT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290831.1
    CDS731..5464
    Translation

    Target ORF information:

    RefSeq Version XM_009292556.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292556.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCT ACAGGCAGTA TTCCTCAAAA
    GGCCAGGGTT TAAGTCCAGT GAAATACAAA GGAATGCAAG AGGCCATGCT TGGCAAAGGC
    CGGCTTGGCT CTCGTAGCCA ATCCCTGCGC TCGGTGGACA GCCGTAAACG AGAGGACTCT
    GATAAGACCC GCAGCGGACA GCAGTCTTCC CAAACAGTGG ATTCTCCGTC GGGTGAGGAG
    TGGTCTGCGT GGAGCGTGTG TTCGGCCACG TGTGGTGAGG GCTGGCAGAG CCGCACACGC
    TTCTGCGTCT CCGTGTCGTA CAGCACGCAG TGCAGCGGGC CACTGCGCGA ACAGAGACCC
    TGCAATAACA CTGCTGTGTG TCCAGCCGCT CTGATTACAG AACATGGGGC GTGGGGCGAG
    TGGACACCAT GGAGCTTGTG TTCATCAACA TGCGGCCGTG GCTACAGGGA ACGCAACCGC
    TCCTGCAAGC CACCCCAGTT CGGAGGAAAC CCCTGTGATG GTCCAGAGAA ACAGACTAAA
    TTCTGCAACA TCGCTGTATG CCCAGTGGAT GGCGTGTGGA ACGAGTGGTC TGGTTGGAGT
    TCCTGTTCTT CAACTTGTTC GAATGGAACA ATGCAGCGAA TTCGGGAGTG TAACGGGCCC
    TCGTATGGGG GCTTAGAGTG TCAAGGCGAG TGGCACGAGA CACGGGATTG CTTCCTTAGA
    GAATGTCCTG TGGATGGCCA ATGGCAACTT TGGAGCACTT GGTCTGGTTG CACCAAAACC
    TGTGGTGGAG GAAGTCAGCA GAGACAGAGG CTGTGTTATG GTCCCTTTTT TGGGGGAGAG
    GCATGCCCGG GGGACCGAGA AGAAGTTAGA CGTTGCAATG AGAAGCGATG CCCAGAGCCA
    CATGAAATCT GTGATGAGGA GAATTTCTCT AATGTAGTGT GGAAGAAAAC ACCTGCAGGG
    GACACAGCGG CTGTCCGCTG CCCACCAAAT GCTGTAGGAT TAATTCTACG GCGATGTTCA
    TTAGACAATG AGGGTATAGC ATACTGGGAG AACCCAACAT ATATGAAGTG CGTCTCAAAT
    GATTACAGAA GTATCCAAAC ACTGACCAGA GATCACCTGT CCAAGGCGCA GCGAGGGCTT
    GTGGGGGATG GTGTTTCTGA AGTGATGACA AAGCTGAGGG TGACGTCAAG TGATGGAACG
    AGCTACAGCG GAGATCTTTT GGCTATCATA GATGTGCTAA AAAACATGAC AGAGATATTT
    AGGAGATCCT ACTACAGCCC CAGTAATGCA GACATGAGGA ATTTTGTGCA GTCTGTGAGT
    AACTTACTGA TGGAGGAAAA CAGGGACAGG TGGGAGGAAG CACAACTGCT GGGACCCAAT
    GTTAAGGAGC TTTTTCGTCT AGTAGAGGAC TTTGTGGACG TCATCAGCCT TCGCATGAAG
    GACTTCCAGG ATACTTATGA GGTCACGGAT AATCTAGTGT TGAGCATTCA CAAGCGGCCC
    GTGGCTGGAA ATGCAGATAT CACTTTCCCT ATGAAGGGCT GGAGGGGGAT GGTGGACTGG
    GCTCGCAGCT CTGAGGACAA AGTCAGAGTG GCCAAAAACA TCCTGCTCAC TGGAAAACCG
    GAAGAAGATG ACGCTTCAGC TTTTGTGACT GGGATCGTCC TGTACAGAAA CTTAGGCAGT
    ATTCTCTCTC TGCAGAGAAA TAGCACAGTG CTGAACTCCA AGGTGATCTC TGTGACAATC
    AAGCCCACTC CCAGCTCTCT CTCGAGTCCA CTAGAGATTG AGTTCTCACA CCTTTACAAT
    GGAACCACAA ACCAAACCTG CATTTCCTGG GATGAGAACG ACAGCTCGTC GTTGCTGGGC
    TCCTGGTCTG CGCGAGGCTG TAGAGCCGTG CCCGTCGACT CCAGCACAAC CAAATGCTTG
    TGTGACAGAC TCTCCACCTT CGCCATTTTA GCACGCCTCA ATCCCGATGT GAACATGGAT
    AAGACCCAGC TTCCATCTGT GACTCTGATC GTGGGCTGCG GGGTCTCGTC CCTCACCCTG
    CTCCTCCTCA TCATCATCTA TGTTTCTGTT TGGAGGTACA TCCGCTCAGA GCGCTCTGTC
    ATCCTCATCA ACTTCTGTCT GTCCATCATC TCCTCCAATG CGCTTATACT CATCGGTCAA
    ACTCAGACAC GGAACAAGGT TGTTTGTACT CTCGTGGCTG CGTTTCTGCA CTTTTTCTTC
    CTGTCCTCTT TCTGTTGGGT TCTAACGGAG GCATGGCAGT CGTACATGGC TGTGACCGGC
    CGCCTTAGAA ACCGTATCAT ACGCAAGCGT TTCCTTTGCC TTGGTTGGGG TTTACCTGCT
    CTCGTTGTAG CAATTTCTGT TGGATTCACC AAGGCAAAGG GATATGGCAC TGTTAACTAT
    TGCTGGCTGT CTCTGGAAGG AGGGCTGTTG TACGCATTTG TGGGACCAGC TGCTGCTGTC
    GTCCTGGTTA ACATGGTTAT CGGGATTCTG GTTTTTAACA AACTGGTGTC CAAAGATGGC
    ATTACTGATA TGAAACTCAA GGAGAGGGCC GGGGCGTCGC TGTGGAGCTC CTGCGTTGTT
    CTGCCGTTGC TGGCGCTCAC TTGGATGTCG GCAGTCTTGG CTATTACTGA CCGCCGTTCA
    GCCTTGTTTC AAATCCTTTT TGCCGTCTTT GACTCCCTCG AGGGCTTTGT TATCGTGATG
    GTGCATTGCA TCCTACGCAG AGAGGTTCAA GAAGCAGTAA AGTGTCGTGT TGTTGACCGT
    CAAGAAGATG GAAACGGTGA CTCTGGTGGA TCCTTCCAGA ACGGTCATGC TCAGCTGATG
    ACTGACTTTG AAAAGGATGT GGACATAGCT TGTCGATCAG GCACCCTGAA GCGTCCCTCC
    CAGCAAGGCG AGGAGAAGGT TCCATCCCAG CAGATCACAG TACAGAAAGG CTCCAACTTC
    AACACCCTTC CTGCCAGCAT GGCAAAGGTC CACCTGCAAA ACGTAGCCAA CTACGCCAGT
    CACACTCTCA CCATGCGCCG CGACAAAGGC GCCATCGCCG GGGTCAGCAC GGAGTTACCC
    GGGGCCAAAT CTATTTACAT CTGTGACGGC GAGCTCTTCA AACAGCTTGA CGGAGCACCG
    GGAGACATTC CTGAAGGAGG TGGTGGAGGT GCAGGTCAGG GTTATGTGCT TTTGCCCAAC
    AACAACAACA CCCTTAGGCC TACGAAGGGT GGCAAGGAAG ACCCGGCCGC CAAATATAAC
    ATCAGTATCG AACAGTTACC CCAGACAAGG CTGATCCACC TGGCCAATCC TGTTGGCACC
    GATCCCGTGG CAGGCTTCGG GCTGAAGAGC TTGCCCACCG ACCGAGTCAG CGTTTCATGC
    TCGGAGAGGG AGTCCCCGGT GCAGACGGTG CAGAATATCT CCAGCGAGTC CCAGATGACA
    AATACGTGTG AGCAAGGGGA TTCTGGGAAC TCTGGGATGT CTAAGAGCGA GACTGTCTCC
    ACACTGTCCA TGAGCTCTCT GGAGAGACGA AAATCACGCT ATGCAGAGCT GGACTTTGAG
    AAGATCATGC ACACACGGAA GCGTCATCAG GACATGTTTC AGGACCTGAA TCGCAAACTG
    CAGCACGCAG AGAAGGACAG AGACTCTCCT CCAGTCGACT GCAAGCCTGG AAAGAGATGG
    AGCATTTCGT CAGGAGGAAG CGACAAAACC AACCTTAGTG ACAAGCAGCA GACCCCCAGT
    AAGCGGGCAT GGGAGGGCAT CAGGAAGACT CACTCTCCTC CATCATGGGT GCGCAAGGAG
    CTGGAGCCGC TGCAGGCCTC ACCGCTGGAG ATGCAGGCAG TGGAGTGGGA GAAGGCCAGT
    GCCACCATCC CTCTAGTGGG CCAGGAGATC ATGGACCTGC AGACGGAGGT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44099
    Clone ID Related Accession (Same CDS sequence) XM_009292556.2 , XM_009292556.3
    Accession Version XM_009292556.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4734bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product brain-specific angiogenesis inhibitor 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009292556.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCT ACAGGCAGTA TTCCTCAAAA
    GGCCAGGGTT TAAGTCCAGT GAAATACAAA GGAATGCAAG AGGCCATGCT TGGCAAAGGC
    CGGCTTGGCT CTCGTAGCCA ATCCCTGCGC TCGGTGGACA GCCGTAAACG AGAGGACTCT
    GATAAGACCC GCAGCGGACA GCAGTCTTCC CAAACAGTGG ATTCTCCGTC GGGTGAGGAG
    TGGTCTGCGT GGAGCGTGTG TTCGGCCACG TGTGGTGAGG GCTGGCAGAG CCGCACACGC
    TTCTGCGTCT CCGTGTCGTA CAGCACGCAG TGCAGCGGGC CACTGCGCGA ACAGAGACCC
    TGCAATAACA CTGCTGTGTG TCCAGCCGCT CTGATTACAG AACATGGGGC GTGGGGCGAG
    TGGACACCAT GGAGCTTGTG TTCATCAACA TGCGGCCGTG GCTACAGGGA ACGCAACCGC
    TCCTGCAAGC CACCCCAGTT CGGAGGAAAC CCCTGTGATG GTCCAGAGAA ACAGACTAAA
    TTCTGCAACA TCGCTGTATG CCCAGTGGAT GGCGTGTGGA ACGAGTGGTC TGGTTGGAGT
    TCCTGTTCTT CAACTTGTTC GAATGGAACA ATGCAGCGAA TTCGGGAGTG TAACGGGCCC
    TCGTATGGGG GCTTAGAGTG TCAAGGCGAG TGGCACGAGA CACGGGATTG CTTCCTTAGA
    GAATGTCCTG TGGATGGCCA ATGGCAACTT TGGAGCACTT GGTCTGGTTG CACCAAAACC
    TGTGGTGGAG GAAGTCAGCA GAGACAGAGG CTGTGTTATG GTCCCTTTTT TGGGGGAGAG
    GCATGCCCGG GGGACCGAGA AGAAGTTAGA CGTTGCAATG AGAAGCGATG CCCAGAGCCA
    CATGAAATCT GTGATGAGGA GAATTTCTCT AATGTAGTGT GGAAGAAAAC ACCTGCAGGG
    GACACAGCGG CTGTCCGCTG CCCACCAAAT GCTGTAGGAT TAATTCTACG GCGATGTTCA
    TTAGACAATG AGGGTATAGC ATACTGGGAG AACCCAACAT ATATGAAGTG CGTCTCAAAT
    GATTACAGAA GTATCCAAAC ACTGACCAGA GATCACCTGT CCAAGGCGCA GCGAGGGCTT
    GTGGGGGATG GTGTTTCTGA AGTGATGACA AAGCTGAGGG TGACGTCAAG TGATGGAACG
    AGCTACAGCG GAGATCTTTT GGCTATCATA GATGTGCTAA AAAACATGAC AGAGATATTT
    AGGAGATCCT ACTACAGCCC CAGTAATGCA GACATGAGGA ATTTTGTGCA GTCTGTGAGT
    AACTTACTGA TGGAGGAAAA CAGGGACAGG TGGGAGGAAG CACAACTGCT GGGACCCAAT
    GTTAAGGAGC TTTTTCGTCT AGTAGAGGAC TTTGTGGACG TCATCAGCCT TCGCATGAAG
    GACTTCCAGG ATACTTATGA GGTCACGGAT AATCTAGTGT TGAGCATTCA CAAGCGGCCC
    GTGGCTGGAA ATGCAGATAT CACTTTCCCT ATGAAGGGCT GGAGGGGGAT GGTGGACTGG
    GCTCGCAGCT CTGAGGACAA AGTCAGAGTG GCCAAAAACA TCCTGCTCAC TGGAAAACCG
    GAAGAAGATG ACGCTTCAGC TTTTGTGACT GGGATCGTCC TGTACAGAAA CTTAGGCAGT
    ATTCTCTCTC TGCAGAGAAA TAGCACAGTG CTGAACTCCA AGGTGATCTC TGTGACAATC
    AAGCCCACTC CCAGCTCTCT CTCGAGTCCA CTAGAGATTG AGTTCTCACA CCTTTACAAT
    GGAACCACAA ACCAAACCTG CATTTCCTGG GATGAGAACG ACAGCTCGTC GTTGCTGGGC
    TCCTGGTCTG CGCGAGGCTG TAGAGCCGTG CCCGTCGACT CCAGCACAAC CAAATGCTTG
    TGTGACAGAC TCTCCACCTT CGCCATTTTA GCACGCCTCA ATCCCGATGT GAACATGGAT
    AAGACCCAGC TTCCATCTGT GACTCTGATC GTGGGCTGCG GGGTCTCGTC CCTCACCCTG
    CTCCTCCTCA TCATCATCTA TGTTTCTGTT TGGAGGTACA TCCGCTCAGA GCGCTCTGTC
    ATCCTCATCA ACTTCTGTCT GTCCATCATC TCCTCCAATG CGCTTATACT CATCGGTCAA
    ACTCAGACAC GGAACAAGGT TGTTTGTACT CTCGTGGCTG CGTTTCTGCA CTTTTTCTTC
    CTGTCCTCTT TCTGTTGGGT TCTAACGGAG GCATGGCAGT CGTACATGGC TGTGACCGGC
    CGCCTTAGAA ACCGTATCAT ACGCAAGCGT TTCCTTTGCC TTGGTTGGGG TTTACCTGCT
    CTCGTTGTAG CAATTTCTGT TGGATTCACC AAGGCAAAGG GATATGGCAC TGTTAACTAT
    TGCTGGCTGT CTCTGGAAGG AGGGCTGTTG TACGCATTTG TGGGACCAGC TGCTGCTGTC
    GTCCTGGTTA ACATGGTTAT CGGGATTCTG GTTTTTAACA AACTGGTGTC CAAAGATGGC
    ATTACTGATA TGAAACTCAA GGAGAGGGCC GGGGCGTCGC TGTGGAGCTC CTGCGTTGTT
    CTGCCGTTGC TGGCGCTCAC TTGGATGTCG GCAGTCTTGG CTATTACTGA CCGCCGTTCA
    GCCTTGTTTC AAATCCTTTT TGCCGTCTTT GACTCCCTCG AGGGCTTTGT TATCGTGATG
    GTGCATTGCA TCCTACGCAG AGAGGTTCAA GAAGCAGTAA AGTGTCGTGT TGTTGACCGT
    CAAGAAGATG GAAACGGTGA CTCTGGTGGA TCCTTCCAGA ACGGTCATGC TCAGCTGATG
    ACTGACTTTG AAAAGGATGT GGACATAGCT TGTCGATCAG GCACCCTGAA GCGTCCCTCC
    CAGCAAGGCG AGGAGAAGGT TCCATCCCAG CAGATCACAG TACAGAAAGG CTCCAACTTC
    AACACCCTTC CTGCCAGCAT GGCAAAGGTC CACCTGCAAA ACGTAGCCAA CTACGCCAGT
    CACACTCTCA CCATGCGCCG CGACAAAGGC GCCATCGCCG GGGTCAGCAC GGAGTTACCC
    GGGGCCAAAT CTATTTACAT CTGTGACGGC GAGCTCTTCA AACAGCTTGA CGGAGCACCG
    GGAGACATTC CTGAAGGAGG TGGTGGAGGT GCAGGTCAGG GTTATGTGCT TTTGCCCAAC
    AACAACAACA CCCTTAGGCC TACGAAGGGT GGCAAGGAAG ACCCGGCCGC CAAATATAAC
    ATCAGTATCG AACAGTTACC CCAGACAAGG CTGATCCACC TGGCCAATCC TGTTGGCACC
    GATCCCGTGG CAGGCTTCGG GCTGAAGAGC TTGCCCACCG ACCGAGTCAG CGTTTCATGC
    TCGGAGAGGG AGTCCCCGGT GCAGACGGTG CAGAATATCT CCAGCGAGTC CCAGATGACA
    AATACGTGTG AGCAAGGGGA TTCTGGGAAC TCTGGGATGT CTAAGAGCGA GACTGTCTCC
    ACACTGTCCA TGAGCTCTCT GGAGAGACGA AAATCACGCT ATGCAGAGCT GGACTTTGAG
    AAGATCATGC ACACACGGAA GCGTCATCAG GACATGTTTC AGGACCTGAA TCGCAAACTG
    CAGCACGCAG AGAAGGACAG AGACTCTCCT CCAGTCGACT GCAAGCCTGG AAAGAGATGG
    AGCATTTCGT CAGGAGGAAG CGACAAAACC AACCTTAGTG ACAAGCAGCA GACCCCCAGT
    AAGCGGGCAT GGGAGGGCAT CAGGAAGACT CACTCTCCTC CATCATGGGT GCGCAAGGAG
    CTGGAGCCGC TGCAGGCCTC ACCGCTGGAG ATGCAGGCAG TGGAGTGGGA GAAGGCCAGT
    GCCACCATCC CTCTAGTGGG CCAGGAGATC ATGGACCTGC AGACGGAGGT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290831.1
    CDS747..5480
    Translation

    Target ORF information:

    RefSeq Version XM_009292556.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292556.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCT ACAGGCAGTA TTCCTCAAAA
    GGCCAGGGTT TAAGTCCAGT GAAATACAAA GGAATGCAAG AGGCCATGCT TGGCAAAGGC
    CGGCTTGGCT CTCGTAGCCA ATCCCTGCGC TCGGTGGACA GCCGTAAACG AGAGGACTCT
    GATAAGACCC GCAGCGGACA GCAGTCTTCC CAAACAGTGG ATTCTCCGTC GGGTGAGGAG
    TGGTCTGCGT GGAGCGTGTG TTCGGCCACG TGTGGTGAGG GCTGGCAGAG CCGCACACGC
    TTCTGCGTCT CCGTGTCGTA CAGCACGCAG TGCAGCGGGC CACTGCGCGA ACAGAGACCC
    TGCAATAACA CTGCTGTGTG TCCAGCCGCT CTGATTACAG AACATGGGGC GTGGGGCGAG
    TGGACACCAT GGAGCTTGTG TTCATCAACA TGCGGCCGTG GCTACAGGGA ACGCAACCGC
    TCCTGCAAGC CACCCCAGTT CGGAGGAAAC CCCTGTGATG GTCCAGAGAA ACAGACTAAA
    TTCTGCAACA TCGCTGTATG CCCAGTGGAT GGCGTGTGGA ACGAGTGGTC TGGTTGGAGT
    TCCTGTTCTT CAACTTGTTC GAATGGAACA ATGCAGCGAA TTCGGGAGTG TAACGGGCCC
    TCGTATGGGG GCTTAGAGTG TCAAGGCGAG TGGCACGAGA CACGGGATTG CTTCCTTAGA
    GAATGTCCTG TGGATGGCCA ATGGCAACTT TGGAGCACTT GGTCTGGTTG CACCAAAACC
    TGTGGTGGAG GAAGTCAGCA GAGACAGAGG CTGTGTTATG GTCCCTTTTT TGGGGGAGAG
    GCATGCCCGG GGGACCGAGA AGAAGTTAGA CGTTGCAATG AGAAGCGATG CCCAGAGCCA
    CATGAAATCT GTGATGAGGA GAATTTCTCT AATGTAGTGT GGAAGAAAAC ACCTGCAGGG
    GACACAGCGG CTGTCCGCTG CCCACCAAAT GCTGTAGGAT TAATTCTACG GCGATGTTCA
    TTAGACAATG AGGGTATAGC ATACTGGGAG AACCCAACAT ATATGAAGTG CGTCTCAAAT
    GATTACAGAA GTATCCAAAC ACTGACCAGA GATCACCTGT CCAAGGCGCA GCGAGGGCTT
    GTGGGGGATG GTGTTTCTGA AGTGATGACA AAGCTGAGGG TGACGTCAAG TGATGGAACG
    AGCTACAGCG GAGATCTTTT GGCTATCATA GATGTGCTAA AAAACATGAC AGAGATATTT
    AGGAGATCCT ACTACAGCCC CAGTAATGCA GACATGAGGA ATTTTGTGCA GTCTGTGAGT
    AACTTACTGA TGGAGGAAAA CAGGGACAGG TGGGAGGAAG CACAACTGCT GGGACCCAAT
    GTTAAGGAGC TTTTTCGTCT AGTAGAGGAC TTTGTGGACG TCATCAGCCT TCGCATGAAG
    GACTTCCAGG ATACTTATGA GGTCACGGAT AATCTAGTGT TGAGCATTCA CAAGCGGCCC
    GTGGCTGGAA ATGCAGATAT CACTTTCCCT ATGAAGGGCT GGAGGGGGAT GGTGGACTGG
    GCTCGCAGCT CTGAGGACAA AGTCAGAGTG GCCAAAAACA TCCTGCTCAC TGGAAAACCG
    GAAGAAGATG ACGCTTCAGC TTTTGTGACT GGGATCGTCC TGTACAGAAA CTTAGGCAGT
    ATTCTCTCTC TGCAGAGAAA TAGCACAGTG CTGAACTCCA AGGTGATCTC TGTGACAATC
    AAGCCCACTC CCAGCTCTCT CTCGAGTCCA CTAGAGATTG AGTTCTCACA CCTTTACAAT
    GGAACCACAA ACCAAACCTG CATTTCCTGG GATGAGAACG ACAGCTCGTC GTTGCTGGGC
    TCCTGGTCTG CGCGAGGCTG TAGAGCCGTG CCCGTCGACT CCAGCACAAC CAAATGCTTG
    TGTGACAGAC TCTCCACCTT CGCCATTTTA GCACGCCTCA ATCCCGATGT GAACATGGAT
    AAGACCCAGC TTCCATCTGT GACTCTGATC GTGGGCTGCG GGGTCTCGTC CCTCACCCTG
    CTCCTCCTCA TCATCATCTA TGTTTCTGTT TGGAGGTACA TCCGCTCAGA GCGCTCTGTC
    ATCCTCATCA ACTTCTGTCT GTCCATCATC TCCTCCAATG CGCTTATACT CATCGGTCAA
    ACTCAGACAC GGAACAAGGT TGTTTGTACT CTCGTGGCTG CGTTTCTGCA CTTTTTCTTC
    CTGTCCTCTT TCTGTTGGGT TCTAACGGAG GCATGGCAGT CGTACATGGC TGTGACCGGC
    CGCCTTAGAA ACCGTATCAT ACGCAAGCGT TTCCTTTGCC TTGGTTGGGG TTTACCTGCT
    CTCGTTGTAG CAATTTCTGT TGGATTCACC AAGGCAAAGG GATATGGCAC TGTTAACTAT
    TGCTGGCTGT CTCTGGAAGG AGGGCTGTTG TACGCATTTG TGGGACCAGC TGCTGCTGTC
    GTCCTGGTTA ACATGGTTAT CGGGATTCTG GTTTTTAACA AACTGGTGTC CAAAGATGGC
    ATTACTGATA TGAAACTCAA GGAGAGGGCC GGGGCGTCGC TGTGGAGCTC CTGCGTTGTT
    CTGCCGTTGC TGGCGCTCAC TTGGATGTCG GCAGTCTTGG CTATTACTGA CCGCCGTTCA
    GCCTTGTTTC AAATCCTTTT TGCCGTCTTT GACTCCCTCG AGGGCTTTGT TATCGTGATG
    GTGCATTGCA TCCTACGCAG AGAGGTTCAA GAAGCAGTAA AGTGTCGTGT TGTTGACCGT
    CAAGAAGATG GAAACGGTGA CTCTGGTGGA TCCTTCCAGA ACGGTCATGC TCAGCTGATG
    ACTGACTTTG AAAAGGATGT GGACATAGCT TGTCGATCAG GCACCCTGAA GCGTCCCTCC
    CAGCAAGGCG AGGAGAAGGT TCCATCCCAG CAGATCACAG TACAGAAAGG CTCCAACTTC
    AACACCCTTC CTGCCAGCAT GGCAAAGGTC CACCTGCAAA ACGTAGCCAA CTACGCCAGT
    CACACTCTCA CCATGCGCCG CGACAAAGGC GCCATCGCCG GGGTCAGCAC GGAGTTACCC
    GGGGCCAAAT CTATTTACAT CTGTGACGGC GAGCTCTTCA AACAGCTTGA CGGAGCACCG
    GGAGACATTC CTGAAGGAGG TGGTGGAGGT GCAGGTCAGG GTTATGTGCT TTTGCCCAAC
    AACAACAACA CCCTTAGGCC TACGAAGGGT GGCAAGGAAG ACCCGGCCGC CAAATATAAC
    ATCAGTATCG AACAGTTACC CCAGACAAGG CTGATCCACC TGGCCAATCC TGTTGGCACC
    GATCCCGTGG CAGGCTTCGG GCTGAAGAGC TTGCCCACCG ACCGAGTCAG CGTTTCATGC
    TCGGAGAGGG AGTCCCCGGT GCAGACGGTG CAGAATATCT CCAGCGAGTC CCAGATGACA
    AATACGTGTG AGCAAGGGGA TTCTGGGAAC TCTGGGATGT CTAAGAGCGA GACTGTCTCC
    ACACTGTCCA TGAGCTCTCT GGAGAGACGA AAATCACGCT ATGCAGAGCT GGACTTTGAG
    AAGATCATGC ACACACGGAA GCGTCATCAG GACATGTTTC AGGACCTGAA TCGCAAACTG
    CAGCACGCAG AGAAGGACAG AGACTCTCCT CCAGTCGACT GCAAGCCTGG AAAGAGATGG
    AGCATTTCGT CAGGAGGAAG CGACAAAACC AACCTTAGTG ACAAGCAGCA GACCCCCAGT
    AAGCGGGCAT GGGAGGGCAT CAGGAAGACT CACTCTCCTC CATCATGGGT GCGCAAGGAG
    CTGGAGCCGC TGCAGGCCTC ACCGCTGGAG ATGCAGGCAG TGGAGTGGGA GAAGGCCAGT
    GCCACCATCC CTCTAGTGGG CCAGGAGATC ATGGACCTGC AGACGGAGGT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44101
    Clone ID Related Accession (Same CDS sequence) XM_009292559.2 , XM_009292559.3
    Accession Version XM_009292559.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4665bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product brain-specific angiogenesis inhibitor 1 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292559.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCT ACAGGCAGTA TTCCTCAAAA
    GGCCAGGGTT TAAGTCCAGT GAAATACAAA GGAATGCAAG AGGCCATGCT TGGCAAAGGC
    CGGCTTGGCT CTCGTAGCCA ATCCCTGCGC TCGGTGGACA GCCGTAAACG AGAGGACTCT
    GATAAGACCC GCAGCGGACA GCAGTCTTCC CAAACAGCCG CTCTGATTAC AGAACATGGG
    GCGTGGGGCG AGTGGACACC ATGGAGCTTG TGTTCATCAA CATGCGGCCG TGGCTACAGG
    GAACGCAACC GCTCCTGCAA GCCACCCCAG TTCGGAGGAA ACCCCTGTGA TGGTCCAGAG
    AAACAGACTA AATTCTGCAA CATCGCTGTA TGCCCAGTGG ATGGCGTGTG GAACGAGTGG
    TCTGGTTGGA GTTCCTGTTC TTCAACTTGT TCGAATGGAA CAATGCAGCG AATTCGGGAG
    TGTAACGGGC CCTCGTATGG GGGCTTAGAG TGTCAAGGCG AGTGGCACGA GACACGGGAT
    TGCTTCCTTA GAGAATGTCC TGTGGATGGC CAATGGCAAC TTTGGAGCAC TTGGTCTGGT
    TGCACCAAAA CCTGTGGTGG AGGAAGTCAG CAGAGACAGA GGCTGTGTTA TGGTCCCTTT
    TTTGGGGGAG AGGCATGCCC GGGGGACCGA GAAGAAGTTA GACGTTGCAA TGAGAAGCGA
    TGCCCAGAGC CACATGAAAT CTGTGATGAG GAGAATTTCT CTAATGTAGT GTGGAAGAAA
    ACACCTGCAG GGGACACAGC GGCTGTCCGC TGCCCACCAA ATGCTGTAGG ATTAATTCTA
    CGGCGATGTT CATTAGACAA TGAGGGTATA GCATACTGGG AGAACCCAAC ATATATGAAG
    TGCGTCTCAA ATGATTACAG AAGTATCCAA ACACTGACCA GAGATCACCT GTCCAAGGCG
    CAGCGAGGGC TTGTGGGGGA TGGTGTTTCT GAAGTGATGA CAAAGCTGAG GGTGACGTCA
    AGTGATGGAA CGAGCTACAG CGGAGATCTT TTGGCTATCA TAGATGTGCT AAAAAACATG
    ACAGAGATAT TTAGGAGATC CTACTACAGC CCCAGTAATG CAGACATGAG GAATTTTGTG
    CAGTCTGTGA GTAACTTACT GATGGAGGAA AACAGGGACA GGTGGGAGGA AGCACAACTG
    CTGGGACCCA ATGTTAAGGA GCTTTTTCGT CTAGTAGAGG ACTTTGTGGA CGTCATCAGC
    CTTCGCATGA AGGACTTCCA GGATACTTAT GAGGTCACGG ATAATCTAGT GTTGAGCATT
    CACAAGCGGC CCGTGGCTGG AAATGCAGAT ATCACTTTCC CTATGAAGGG CTGGAGGGGG
    ATGGTGGACT GGGCTCGCAG CTCTGAGGAC AAAGTCAGAG TGGCCAAAAA CATCCTGCTC
    ACTGGAAAAC CGGAAGAAGA TGACGCTTCA GCTTTTGTGA CTGGGATCGT CCTGTACAGA
    AACTTAGGCA GTATTCTCTC TCTGCAGAGA AATAGCACAG TGCTGAACTC CAAGGTGATC
    TCTGTGACAA TCAAGCCCAC TCCCAGCTCT CTCTCGAGTC CACTAGAGAT TGAGTTCTCA
    CACCTTTACA ATGGAACCAC AAACCAAACC TGCATTTCCT GGGATGAGAA CGACAGCTCG
    TCGTTGCTGG GCTCCTGGTC TGCGCGAGGC TGTAGAGCCG TGCCCGTCGA CTCCAGCACA
    ACCAAATGCT TGTGTGACAG ACTCTCCACC TTCGCCATTT TAGCACGCCT CAATCCCGAT
    GTGAACATGG ATAAGACCCA GCTTCCATCT GTGACTCTGA TCGTGGGCTG CGGGGTCTCG
    TCCCTCACCC TGCTCCTCCT CATCATCATC TATGTTTCTG TTTGGAGGTA CATCCGCTCA
    GAGCGCTCTG TCATCCTCAT CAACTTCTGT CTGTCCATCA TCTCCTCCAA TGCGCTTATA
    CTCATCGGTC AAACTCAGAC ACGGAACAAG GTTGTTTGTA CTCTCGTGGC TGCGTTTCTG
    CACTTTTTCT TCCTGTCCTC TTTCTGTTGG GTTCTAACGG AGGCATGGCA GTCGTACATG
    GCTGTGACCG GCCGCCTTAG AAACCGTATC ATACGCAAGC GTTTCCTTTG CCTTGGTTGG
    GGTTTACCTG CTCTCGTTGT AGCAATTTCT GTTGGATTCA CCAAGGCAAA GGGATATGGC
    ACTGTTAACT ATTGCTGGCT GTCTCTGGAA GGAGGGCTGT TGTACGCATT TGTGGGACCA
    GCTGCTGCTG TCGTCCTGGT TAACATGGTT ATCGGGATTC TGGTTTTTAA CAAACTGGTG
    TCCAAAGATG GCATTACTGA TATGAAACTC AAGGAGAGGG CCGGTCAGAT GACAGTGCCA
    CTGTACAATA TGACGCTCAA ATGTGCCAAG TGCGGAGTTA TCTCGTCGCC TGACGTTTCC
    ACCACAGCTA CCAGTAACGC CATGGCGTCG CTGTGGAGCT CCTGCGTTGT TCTGCCGTTG
    CTGGCGCTCA CTTGGATGTC GGCAGTCTTG GCTATTACTG ACCGCCGTTC AGCCTTGTTT
    CAAATCCTTT TTGCCGTCTT TGACTCCCTC GAGGGCTTTG TTATCGTGAT GGTGCATTGC
    ATCCTACGCA GAGAGGTTCA AGAAGCAGTA AAGTGTCGTG TTGTTGACCG TCAAGAAGAT
    GGAAACGGTG ACTCTGGTGG ATCCTTCCAG AACGGTCATG CTCAGCTGAT GACTGACTTT
    GAAAAGGATG TGGACATAGC TTGTCGATCA GGCACCCTGA AGCGTCCCTC CCAGCAAGGC
    GAGGAGAAGG TTCCATCCCA GCAGATCACA GTACAGAAAG GCTCCAACTT CAACACCCTT
    CCTGCCAGCA TGGCAAAGGT CCACCTGCAA AACGTAGCCA ACTACGCCAG TCACACTCTC
    ACCATGCGCC GCGACAAAGG CGCCATCGCC GGGGTCAGCA CGGAGTTACC CGGGGCCAAA
    TCTATTTACA TCTGTGACGG CGAGCTCTTC AAACAGCTTG ACGGAGCACC GGGAGACATT
    CCTGAAGGAG GTGGTGGAGG TGCAGGTCAG GGTTATGTGC TTTTGCCCAA CAACAACAAC
    ACCCTTAGGC CTACGAAGGG TGGCAAGGAA GACCCGGCCG CCAAATATAA CATCAGTATC
    GAACAGTTAC CCCAGACAAG GCTGATCCAC CTGGCCAATC CTGTTGGCAC CGATCCCGTG
    GCAGGCTTCG GGCTGAAGAG CTTGCCCACC GACCGAGTCA GCGTTTCATG CTCGGAGAGG
    GAGTCCCCGG TGCAGACGGT GCAGAATATC TCCAGCGAGT CCCAGATGAC AAATACGTGT
    GAGCAAGGGG ATTCTGGGAA CTCTGGGATG TCTAAGAGCG AGACTGTCTC CACACTGTCC
    ATGAGCTCTC TGGAGAGACG AAAATCACGC TATGCAGAGC TGGACTTTGA GAAGATCATG
    CACACACGGA AGCGTCATCA GGACATGTTT CAGGACCTGA ATCGCAAACT GCAGCACGCA
    GAGAAGGACA GAGACTCTCC TCCAGTCGAC TGCAAGCCTG GAAAGAGATG GAGCATTTCG
    TCAGGAGGAA GCGACAAAAC CAACCTTAGT GACAAGCAGC AGACCCCCAG TAAGCGGGCA
    TGGGAGGGCA TCAGGAAGAC TCACTCTCCT CCATCATGGG TGCGCAAGGA GCTGGAGCCG
    CTGCAGGCCT CACCGCTGGA GATGCAGGCA GTGGAGTGGG AGAAGGCCAG TGCCACCATC
    CCTCTAGTGG GCCAGGAGAT CATGGACCTG CAGACGGAGG TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290834.1
    CDS731..5395
    Translation

    Target ORF information:

    RefSeq Version XM_009292559.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292559.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCT ACAGGCAGTA TTCCTCAAAA
    GGCCAGGGTT TAAGTCCAGT GAAATACAAA GGAATGCAAG AGGCCATGCT TGGCAAAGGC
    CGGCTTGGCT CTCGTAGCCA ATCCCTGCGC TCGGTGGACA GCCGTAAACG AGAGGACTCT
    GATAAGACCC GCAGCGGACA GCAGTCTTCC CAAACAGCCG CTCTGATTAC AGAACATGGG
    GCGTGGGGCG AGTGGACACC ATGGAGCTTG TGTTCATCAA CATGCGGCCG TGGCTACAGG
    GAACGCAACC GCTCCTGCAA GCCACCCCAG TTCGGAGGAA ACCCCTGTGA TGGTCCAGAG
    AAACAGACTA AATTCTGCAA CATCGCTGTA TGCCCAGTGG ATGGCGTGTG GAACGAGTGG
    TCTGGTTGGA GTTCCTGTTC TTCAACTTGT TCGAATGGAA CAATGCAGCG AATTCGGGAG
    TGTAACGGGC CCTCGTATGG GGGCTTAGAG TGTCAAGGCG AGTGGCACGA GACACGGGAT
    TGCTTCCTTA GAGAATGTCC TGTGGATGGC CAATGGCAAC TTTGGAGCAC TTGGTCTGGT
    TGCACCAAAA CCTGTGGTGG AGGAAGTCAG CAGAGACAGA GGCTGTGTTA TGGTCCCTTT
    TTTGGGGGAG AGGCATGCCC GGGGGACCGA GAAGAAGTTA GACGTTGCAA TGAGAAGCGA
    TGCCCAGAGC CACATGAAAT CTGTGATGAG GAGAATTTCT CTAATGTAGT GTGGAAGAAA
    ACACCTGCAG GGGACACAGC GGCTGTCCGC TGCCCACCAA ATGCTGTAGG ATTAATTCTA
    CGGCGATGTT CATTAGACAA TGAGGGTATA GCATACTGGG AGAACCCAAC ATATATGAAG
    TGCGTCTCAA ATGATTACAG AAGTATCCAA ACACTGACCA GAGATCACCT GTCCAAGGCG
    CAGCGAGGGC TTGTGGGGGA TGGTGTTTCT GAAGTGATGA CAAAGCTGAG GGTGACGTCA
    AGTGATGGAA CGAGCTACAG CGGAGATCTT TTGGCTATCA TAGATGTGCT AAAAAACATG
    ACAGAGATAT TTAGGAGATC CTACTACAGC CCCAGTAATG CAGACATGAG GAATTTTGTG
    CAGTCTGTGA GTAACTTACT GATGGAGGAA AACAGGGACA GGTGGGAGGA AGCACAACTG
    CTGGGACCCA ATGTTAAGGA GCTTTTTCGT CTAGTAGAGG ACTTTGTGGA CGTCATCAGC
    CTTCGCATGA AGGACTTCCA GGATACTTAT GAGGTCACGG ATAATCTAGT GTTGAGCATT
    CACAAGCGGC CCGTGGCTGG AAATGCAGAT ATCACTTTCC CTATGAAGGG CTGGAGGGGG
    ATGGTGGACT GGGCTCGCAG CTCTGAGGAC AAAGTCAGAG TGGCCAAAAA CATCCTGCTC
    ACTGGAAAAC CGGAAGAAGA TGACGCTTCA GCTTTTGTGA CTGGGATCGT CCTGTACAGA
    AACTTAGGCA GTATTCTCTC TCTGCAGAGA AATAGCACAG TGCTGAACTC CAAGGTGATC
    TCTGTGACAA TCAAGCCCAC TCCCAGCTCT CTCTCGAGTC CACTAGAGAT TGAGTTCTCA
    CACCTTTACA ATGGAACCAC AAACCAAACC TGCATTTCCT GGGATGAGAA CGACAGCTCG
    TCGTTGCTGG GCTCCTGGTC TGCGCGAGGC TGTAGAGCCG TGCCCGTCGA CTCCAGCACA
    ACCAAATGCT TGTGTGACAG ACTCTCCACC TTCGCCATTT TAGCACGCCT CAATCCCGAT
    GTGAACATGG ATAAGACCCA GCTTCCATCT GTGACTCTGA TCGTGGGCTG CGGGGTCTCG
    TCCCTCACCC TGCTCCTCCT CATCATCATC TATGTTTCTG TTTGGAGGTA CATCCGCTCA
    GAGCGCTCTG TCATCCTCAT CAACTTCTGT CTGTCCATCA TCTCCTCCAA TGCGCTTATA
    CTCATCGGTC AAACTCAGAC ACGGAACAAG GTTGTTTGTA CTCTCGTGGC TGCGTTTCTG
    CACTTTTTCT TCCTGTCCTC TTTCTGTTGG GTTCTAACGG AGGCATGGCA GTCGTACATG
    GCTGTGACCG GCCGCCTTAG AAACCGTATC ATACGCAAGC GTTTCCTTTG CCTTGGTTGG
    GGTTTACCTG CTCTCGTTGT AGCAATTTCT GTTGGATTCA CCAAGGCAAA GGGATATGGC
    ACTGTTAACT ATTGCTGGCT GTCTCTGGAA GGAGGGCTGT TGTACGCATT TGTGGGACCA
    GCTGCTGCTG TCGTCCTGGT TAACATGGTT ATCGGGATTC TGGTTTTTAA CAAACTGGTG
    TCCAAAGATG GCATTACTGA TATGAAACTC AAGGAGAGGG CCGGTCAGAT GACAGTGCCA
    CTGTACAATA TGACGCTCAA ATGTGCCAAG TGCGGAGTTA TCTCGTCGCC TGACGTTTCC
    ACCACAGCTA CCAGTAACGC CATGGCGTCG CTGTGGAGCT CCTGCGTTGT TCTGCCGTTG
    CTGGCGCTCA CTTGGATGTC GGCAGTCTTG GCTATTACTG ACCGCCGTTC AGCCTTGTTT
    CAAATCCTTT TTGCCGTCTT TGACTCCCTC GAGGGCTTTG TTATCGTGAT GGTGCATTGC
    ATCCTACGCA GAGAGGTTCA AGAAGCAGTA AAGTGTCGTG TTGTTGACCG TCAAGAAGAT
    GGAAACGGTG ACTCTGGTGG ATCCTTCCAG AACGGTCATG CTCAGCTGAT GACTGACTTT
    GAAAAGGATG TGGACATAGC TTGTCGATCA GGCACCCTGA AGCGTCCCTC CCAGCAAGGC
    GAGGAGAAGG TTCCATCCCA GCAGATCACA GTACAGAAAG GCTCCAACTT CAACACCCTT
    CCTGCCAGCA TGGCAAAGGT CCACCTGCAA AACGTAGCCA ACTACGCCAG TCACACTCTC
    ACCATGCGCC GCGACAAAGG CGCCATCGCC GGGGTCAGCA CGGAGTTACC CGGGGCCAAA
    TCTATTTACA TCTGTGACGG CGAGCTCTTC AAACAGCTTG ACGGAGCACC GGGAGACATT
    CCTGAAGGAG GTGGTGGAGG TGCAGGTCAG GGTTATGTGC TTTTGCCCAA CAACAACAAC
    ACCCTTAGGC CTACGAAGGG TGGCAAGGAA GACCCGGCCG CCAAATATAA CATCAGTATC
    GAACAGTTAC CCCAGACAAG GCTGATCCAC CTGGCCAATC CTGTTGGCAC CGATCCCGTG
    GCAGGCTTCG GGCTGAAGAG CTTGCCCACC GACCGAGTCA GCGTTTCATG CTCGGAGAGG
    GAGTCCCCGG TGCAGACGGT GCAGAATATC TCCAGCGAGT CCCAGATGAC AAATACGTGT
    GAGCAAGGGG ATTCTGGGAA CTCTGGGATG TCTAAGAGCG AGACTGTCTC CACACTGTCC
    ATGAGCTCTC TGGAGAGACG AAAATCACGC TATGCAGAGC TGGACTTTGA GAAGATCATG
    CACACACGGA AGCGTCATCA GGACATGTTT CAGGACCTGA ATCGCAAACT GCAGCACGCA
    GAGAAGGACA GAGACTCTCC TCCAGTCGAC TGCAAGCCTG GAAAGAGATG GAGCATTTCG
    TCAGGAGGAA GCGACAAAAC CAACCTTAGT GACAAGCAGC AGACCCCCAG TAAGCGGGCA
    TGGGAGGGCA TCAGGAAGAC TCACTCTCCT CCATCATGGG TGCGCAAGGA GCTGGAGCCG
    CTGCAGGCCT CACCGCTGGA GATGCAGGCA GTGGAGTGGG AGAAGGCCAG TGCCACCATC
    CCTCTAGTGG GCCAGGAGAT CATGGACCTG CAGACGGAGG TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44101
    Clone ID Related Accession (Same CDS sequence) XM_009292559.2 , XM_009292559.3
    Accession Version XM_009292559.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4665bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product brain-specific angiogenesis inhibitor 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009292559.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCT ACAGGCAGTA TTCCTCAAAA
    GGCCAGGGTT TAAGTCCAGT GAAATACAAA GGAATGCAAG AGGCCATGCT TGGCAAAGGC
    CGGCTTGGCT CTCGTAGCCA ATCCCTGCGC TCGGTGGACA GCCGTAAACG AGAGGACTCT
    GATAAGACCC GCAGCGGACA GCAGTCTTCC CAAACAGCCG CTCTGATTAC AGAACATGGG
    GCGTGGGGCG AGTGGACACC ATGGAGCTTG TGTTCATCAA CATGCGGCCG TGGCTACAGG
    GAACGCAACC GCTCCTGCAA GCCACCCCAG TTCGGAGGAA ACCCCTGTGA TGGTCCAGAG
    AAACAGACTA AATTCTGCAA CATCGCTGTA TGCCCAGTGG ATGGCGTGTG GAACGAGTGG
    TCTGGTTGGA GTTCCTGTTC TTCAACTTGT TCGAATGGAA CAATGCAGCG AATTCGGGAG
    TGTAACGGGC CCTCGTATGG GGGCTTAGAG TGTCAAGGCG AGTGGCACGA GACACGGGAT
    TGCTTCCTTA GAGAATGTCC TGTGGATGGC CAATGGCAAC TTTGGAGCAC TTGGTCTGGT
    TGCACCAAAA CCTGTGGTGG AGGAAGTCAG CAGAGACAGA GGCTGTGTTA TGGTCCCTTT
    TTTGGGGGAG AGGCATGCCC GGGGGACCGA GAAGAAGTTA GACGTTGCAA TGAGAAGCGA
    TGCCCAGAGC CACATGAAAT CTGTGATGAG GAGAATTTCT CTAATGTAGT GTGGAAGAAA
    ACACCTGCAG GGGACACAGC GGCTGTCCGC TGCCCACCAA ATGCTGTAGG ATTAATTCTA
    CGGCGATGTT CATTAGACAA TGAGGGTATA GCATACTGGG AGAACCCAAC ATATATGAAG
    TGCGTCTCAA ATGATTACAG AAGTATCCAA ACACTGACCA GAGATCACCT GTCCAAGGCG
    CAGCGAGGGC TTGTGGGGGA TGGTGTTTCT GAAGTGATGA CAAAGCTGAG GGTGACGTCA
    AGTGATGGAA CGAGCTACAG CGGAGATCTT TTGGCTATCA TAGATGTGCT AAAAAACATG
    ACAGAGATAT TTAGGAGATC CTACTACAGC CCCAGTAATG CAGACATGAG GAATTTTGTG
    CAGTCTGTGA GTAACTTACT GATGGAGGAA AACAGGGACA GGTGGGAGGA AGCACAACTG
    CTGGGACCCA ATGTTAAGGA GCTTTTTCGT CTAGTAGAGG ACTTTGTGGA CGTCATCAGC
    CTTCGCATGA AGGACTTCCA GGATACTTAT GAGGTCACGG ATAATCTAGT GTTGAGCATT
    CACAAGCGGC CCGTGGCTGG AAATGCAGAT ATCACTTTCC CTATGAAGGG CTGGAGGGGG
    ATGGTGGACT GGGCTCGCAG CTCTGAGGAC AAAGTCAGAG TGGCCAAAAA CATCCTGCTC
    ACTGGAAAAC CGGAAGAAGA TGACGCTTCA GCTTTTGTGA CTGGGATCGT CCTGTACAGA
    AACTTAGGCA GTATTCTCTC TCTGCAGAGA AATAGCACAG TGCTGAACTC CAAGGTGATC
    TCTGTGACAA TCAAGCCCAC TCCCAGCTCT CTCTCGAGTC CACTAGAGAT TGAGTTCTCA
    CACCTTTACA ATGGAACCAC AAACCAAACC TGCATTTCCT GGGATGAGAA CGACAGCTCG
    TCGTTGCTGG GCTCCTGGTC TGCGCGAGGC TGTAGAGCCG TGCCCGTCGA CTCCAGCACA
    ACCAAATGCT TGTGTGACAG ACTCTCCACC TTCGCCATTT TAGCACGCCT CAATCCCGAT
    GTGAACATGG ATAAGACCCA GCTTCCATCT GTGACTCTGA TCGTGGGCTG CGGGGTCTCG
    TCCCTCACCC TGCTCCTCCT CATCATCATC TATGTTTCTG TTTGGAGGTA CATCCGCTCA
    GAGCGCTCTG TCATCCTCAT CAACTTCTGT CTGTCCATCA TCTCCTCCAA TGCGCTTATA
    CTCATCGGTC AAACTCAGAC ACGGAACAAG GTTGTTTGTA CTCTCGTGGC TGCGTTTCTG
    CACTTTTTCT TCCTGTCCTC TTTCTGTTGG GTTCTAACGG AGGCATGGCA GTCGTACATG
    GCTGTGACCG GCCGCCTTAG AAACCGTATC ATACGCAAGC GTTTCCTTTG CCTTGGTTGG
    GGTTTACCTG CTCTCGTTGT AGCAATTTCT GTTGGATTCA CCAAGGCAAA GGGATATGGC
    ACTGTTAACT ATTGCTGGCT GTCTCTGGAA GGAGGGCTGT TGTACGCATT TGTGGGACCA
    GCTGCTGCTG TCGTCCTGGT TAACATGGTT ATCGGGATTC TGGTTTTTAA CAAACTGGTG
    TCCAAAGATG GCATTACTGA TATGAAACTC AAGGAGAGGG CCGGTCAGAT GACAGTGCCA
    CTGTACAATA TGACGCTCAA ATGTGCCAAG TGCGGAGTTA TCTCGTCGCC TGACGTTTCC
    ACCACAGCTA CCAGTAACGC CATGGCGTCG CTGTGGAGCT CCTGCGTTGT TCTGCCGTTG
    CTGGCGCTCA CTTGGATGTC GGCAGTCTTG GCTATTACTG ACCGCCGTTC AGCCTTGTTT
    CAAATCCTTT TTGCCGTCTT TGACTCCCTC GAGGGCTTTG TTATCGTGAT GGTGCATTGC
    ATCCTACGCA GAGAGGTTCA AGAAGCAGTA AAGTGTCGTG TTGTTGACCG TCAAGAAGAT
    GGAAACGGTG ACTCTGGTGG ATCCTTCCAG AACGGTCATG CTCAGCTGAT GACTGACTTT
    GAAAAGGATG TGGACATAGC TTGTCGATCA GGCACCCTGA AGCGTCCCTC CCAGCAAGGC
    GAGGAGAAGG TTCCATCCCA GCAGATCACA GTACAGAAAG GCTCCAACTT CAACACCCTT
    CCTGCCAGCA TGGCAAAGGT CCACCTGCAA AACGTAGCCA ACTACGCCAG TCACACTCTC
    ACCATGCGCC GCGACAAAGG CGCCATCGCC GGGGTCAGCA CGGAGTTACC CGGGGCCAAA
    TCTATTTACA TCTGTGACGG CGAGCTCTTC AAACAGCTTG ACGGAGCACC GGGAGACATT
    CCTGAAGGAG GTGGTGGAGG TGCAGGTCAG GGTTATGTGC TTTTGCCCAA CAACAACAAC
    ACCCTTAGGC CTACGAAGGG TGGCAAGGAA GACCCGGCCG CCAAATATAA CATCAGTATC
    GAACAGTTAC CCCAGACAAG GCTGATCCAC CTGGCCAATC CTGTTGGCAC CGATCCCGTG
    GCAGGCTTCG GGCTGAAGAG CTTGCCCACC GACCGAGTCA GCGTTTCATG CTCGGAGAGG
    GAGTCCCCGG TGCAGACGGT GCAGAATATC TCCAGCGAGT CCCAGATGAC AAATACGTGT
    GAGCAAGGGG ATTCTGGGAA CTCTGGGATG TCTAAGAGCG AGACTGTCTC CACACTGTCC
    ATGAGCTCTC TGGAGAGACG AAAATCACGC TATGCAGAGC TGGACTTTGA GAAGATCATG
    CACACACGGA AGCGTCATCA GGACATGTTT CAGGACCTGA ATCGCAAACT GCAGCACGCA
    GAGAAGGACA GAGACTCTCC TCCAGTCGAC TGCAAGCCTG GAAAGAGATG GAGCATTTCG
    TCAGGAGGAA GCGACAAAAC CAACCTTAGT GACAAGCAGC AGACCCCCAG TAAGCGGGCA
    TGGGAGGGCA TCAGGAAGAC TCACTCTCCT CCATCATGGG TGCGCAAGGA GCTGGAGCCG
    CTGCAGGCCT CACCGCTGGA GATGCAGGCA GTGGAGTGGG AGAAGGCCAG TGCCACCATC
    CCTCTAGTGG GCCAGGAGAT CATGGACCTG CAGACGGAGG TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290834.1
    CDS747..5411
    Translation

    Target ORF information:

    RefSeq Version XM_009292559.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292559.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCT ACAGGCAGTA TTCCTCAAAA
    GGCCAGGGTT TAAGTCCAGT GAAATACAAA GGAATGCAAG AGGCCATGCT TGGCAAAGGC
    CGGCTTGGCT CTCGTAGCCA ATCCCTGCGC TCGGTGGACA GCCGTAAACG AGAGGACTCT
    GATAAGACCC GCAGCGGACA GCAGTCTTCC CAAACAGCCG CTCTGATTAC AGAACATGGG
    GCGTGGGGCG AGTGGACACC ATGGAGCTTG TGTTCATCAA CATGCGGCCG TGGCTACAGG
    GAACGCAACC GCTCCTGCAA GCCACCCCAG TTCGGAGGAA ACCCCTGTGA TGGTCCAGAG
    AAACAGACTA AATTCTGCAA CATCGCTGTA TGCCCAGTGG ATGGCGTGTG GAACGAGTGG
    TCTGGTTGGA GTTCCTGTTC TTCAACTTGT TCGAATGGAA CAATGCAGCG AATTCGGGAG
    TGTAACGGGC CCTCGTATGG GGGCTTAGAG TGTCAAGGCG AGTGGCACGA GACACGGGAT
    TGCTTCCTTA GAGAATGTCC TGTGGATGGC CAATGGCAAC TTTGGAGCAC TTGGTCTGGT
    TGCACCAAAA CCTGTGGTGG AGGAAGTCAG CAGAGACAGA GGCTGTGTTA TGGTCCCTTT
    TTTGGGGGAG AGGCATGCCC GGGGGACCGA GAAGAAGTTA GACGTTGCAA TGAGAAGCGA
    TGCCCAGAGC CACATGAAAT CTGTGATGAG GAGAATTTCT CTAATGTAGT GTGGAAGAAA
    ACACCTGCAG GGGACACAGC GGCTGTCCGC TGCCCACCAA ATGCTGTAGG ATTAATTCTA
    CGGCGATGTT CATTAGACAA TGAGGGTATA GCATACTGGG AGAACCCAAC ATATATGAAG
    TGCGTCTCAA ATGATTACAG AAGTATCCAA ACACTGACCA GAGATCACCT GTCCAAGGCG
    CAGCGAGGGC TTGTGGGGGA TGGTGTTTCT GAAGTGATGA CAAAGCTGAG GGTGACGTCA
    AGTGATGGAA CGAGCTACAG CGGAGATCTT TTGGCTATCA TAGATGTGCT AAAAAACATG
    ACAGAGATAT TTAGGAGATC CTACTACAGC CCCAGTAATG CAGACATGAG GAATTTTGTG
    CAGTCTGTGA GTAACTTACT GATGGAGGAA AACAGGGACA GGTGGGAGGA AGCACAACTG
    CTGGGACCCA ATGTTAAGGA GCTTTTTCGT CTAGTAGAGG ACTTTGTGGA CGTCATCAGC
    CTTCGCATGA AGGACTTCCA GGATACTTAT GAGGTCACGG ATAATCTAGT GTTGAGCATT
    CACAAGCGGC CCGTGGCTGG AAATGCAGAT ATCACTTTCC CTATGAAGGG CTGGAGGGGG
    ATGGTGGACT GGGCTCGCAG CTCTGAGGAC AAAGTCAGAG TGGCCAAAAA CATCCTGCTC
    ACTGGAAAAC CGGAAGAAGA TGACGCTTCA GCTTTTGTGA CTGGGATCGT CCTGTACAGA
    AACTTAGGCA GTATTCTCTC TCTGCAGAGA AATAGCACAG TGCTGAACTC CAAGGTGATC
    TCTGTGACAA TCAAGCCCAC TCCCAGCTCT CTCTCGAGTC CACTAGAGAT TGAGTTCTCA
    CACCTTTACA ATGGAACCAC AAACCAAACC TGCATTTCCT GGGATGAGAA CGACAGCTCG
    TCGTTGCTGG GCTCCTGGTC TGCGCGAGGC TGTAGAGCCG TGCCCGTCGA CTCCAGCACA
    ACCAAATGCT TGTGTGACAG ACTCTCCACC TTCGCCATTT TAGCACGCCT CAATCCCGAT
    GTGAACATGG ATAAGACCCA GCTTCCATCT GTGACTCTGA TCGTGGGCTG CGGGGTCTCG
    TCCCTCACCC TGCTCCTCCT CATCATCATC TATGTTTCTG TTTGGAGGTA CATCCGCTCA
    GAGCGCTCTG TCATCCTCAT CAACTTCTGT CTGTCCATCA TCTCCTCCAA TGCGCTTATA
    CTCATCGGTC AAACTCAGAC ACGGAACAAG GTTGTTTGTA CTCTCGTGGC TGCGTTTCTG
    CACTTTTTCT TCCTGTCCTC TTTCTGTTGG GTTCTAACGG AGGCATGGCA GTCGTACATG
    GCTGTGACCG GCCGCCTTAG AAACCGTATC ATACGCAAGC GTTTCCTTTG CCTTGGTTGG
    GGTTTACCTG CTCTCGTTGT AGCAATTTCT GTTGGATTCA CCAAGGCAAA GGGATATGGC
    ACTGTTAACT ATTGCTGGCT GTCTCTGGAA GGAGGGCTGT TGTACGCATT TGTGGGACCA
    GCTGCTGCTG TCGTCCTGGT TAACATGGTT ATCGGGATTC TGGTTTTTAA CAAACTGGTG
    TCCAAAGATG GCATTACTGA TATGAAACTC AAGGAGAGGG CCGGTCAGAT GACAGTGCCA
    CTGTACAATA TGACGCTCAA ATGTGCCAAG TGCGGAGTTA TCTCGTCGCC TGACGTTTCC
    ACCACAGCTA CCAGTAACGC CATGGCGTCG CTGTGGAGCT CCTGCGTTGT TCTGCCGTTG
    CTGGCGCTCA CTTGGATGTC GGCAGTCTTG GCTATTACTG ACCGCCGTTC AGCCTTGTTT
    CAAATCCTTT TTGCCGTCTT TGACTCCCTC GAGGGCTTTG TTATCGTGAT GGTGCATTGC
    ATCCTACGCA GAGAGGTTCA AGAAGCAGTA AAGTGTCGTG TTGTTGACCG TCAAGAAGAT
    GGAAACGGTG ACTCTGGTGG ATCCTTCCAG AACGGTCATG CTCAGCTGAT GACTGACTTT
    GAAAAGGATG TGGACATAGC TTGTCGATCA GGCACCCTGA AGCGTCCCTC CCAGCAAGGC
    GAGGAGAAGG TTCCATCCCA GCAGATCACA GTACAGAAAG GCTCCAACTT CAACACCCTT
    CCTGCCAGCA TGGCAAAGGT CCACCTGCAA AACGTAGCCA ACTACGCCAG TCACACTCTC
    ACCATGCGCC GCGACAAAGG CGCCATCGCC GGGGTCAGCA CGGAGTTACC CGGGGCCAAA
    TCTATTTACA TCTGTGACGG CGAGCTCTTC AAACAGCTTG ACGGAGCACC GGGAGACATT
    CCTGAAGGAG GTGGTGGAGG TGCAGGTCAG GGTTATGTGC TTTTGCCCAA CAACAACAAC
    ACCCTTAGGC CTACGAAGGG TGGCAAGGAA GACCCGGCCG CCAAATATAA CATCAGTATC
    GAACAGTTAC CCCAGACAAG GCTGATCCAC CTGGCCAATC CTGTTGGCAC CGATCCCGTG
    GCAGGCTTCG GGCTGAAGAG CTTGCCCACC GACCGAGTCA GCGTTTCATG CTCGGAGAGG
    GAGTCCCCGG TGCAGACGGT GCAGAATATC TCCAGCGAGT CCCAGATGAC AAATACGTGT
    GAGCAAGGGG ATTCTGGGAA CTCTGGGATG TCTAAGAGCG AGACTGTCTC CACACTGTCC
    ATGAGCTCTC TGGAGAGACG AAAATCACGC TATGCAGAGC TGGACTTTGA GAAGATCATG
    CACACACGGA AGCGTCATCA GGACATGTTT CAGGACCTGA ATCGCAAACT GCAGCACGCA
    GAGAAGGACA GAGACTCTCC TCCAGTCGAC TGCAAGCCTG GAAAGAGATG GAGCATTTCG
    TCAGGAGGAA GCGACAAAAC CAACCTTAGT GACAAGCAGC AGACCCCCAG TAAGCGGGCA
    TGGGAGGGCA TCAGGAAGAC TCACTCTCCT CCATCATGGG TGCGCAAGGA GCTGGAGCCG
    CTGCAGGCCT CACCGCTGGA GATGCAGGCA GTGGAGTGGG AGAAGGCCAG TGCCACCATC
    CCTCTAGTGG GCCAGGAGAT CATGGACCTG CAGACGGAGG TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44102
    Clone ID Related Accession (Same CDS sequence) XM_009292560.3 , XM_009292560.2
    Accession Version XM_009292560.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4650bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product brain-specific angiogenesis inhibitor 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009292560.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCT ACAGGCAGTA TTCCTCAAAA
    GGCCAGGGTT TAAGTCCAGT GAAATACAAA GGAATGCAAG AGGCCATGCT TGGCAAAGGC
    CGGCTTGGCT CTCGTAGCCA ATCCCTGCGC TCGGTGGACA GCCGTAAACG AGAGGACTCT
    GATAAGACCC GCAGCGGACA GCAGTCTTCC CAAACAGAAC ATGGGGCGTG GGGCGAGTGG
    ACACCATGGA GCTTGTGTTC ATCAACATGC GGCCGTGGCT ACAGGGAACG CAACCGCTCC
    TGCAAGCCAC CCCAGTTCGG AGGAAACCCC TGTGATGGTC CAGAGAAACA GACTAAATTC
    TGCAACATCG CTGTATGCCC AGTGGATGGC GTGTGGAACG AGTGGTCTGG TTGGAGTTCC
    TGTTCTTCAA CTTGTTCGAA TGGAACAATG CAGCGAATTC GGGAGTGTAA CGGGCCCTCG
    TATGGGGGCT TAGAGTGTCA AGGCGAGTGG CACGAGACAC GGGATTGCTT CCTTAGAGAA
    TGTCCTGTGG ATGGCCAATG GCAACTTTGG AGCACTTGGT CTGGTTGCAC CAAAACCTGT
    GGTGGAGGAA GTCAGCAGAG ACAGAGGCTG TGTTATGGTC CCTTTTTTGG GGGAGAGGCA
    TGCCCGGGGG ACCGAGAAGA AGTTAGACGT TGCAATGAGA AGCGATGCCC AGAGCCACAT
    GAAATCTGTG ATGAGGAGAA TTTCTCTAAT GTAGTGTGGA AGAAAACACC TGCAGGGGAC
    ACAGCGGCTG TCCGCTGCCC ACCAAATGCT GTAGGATTAA TTCTACGGCG ATGTTCATTA
    GACAATGAGG GTATAGCATA CTGGGAGAAC CCAACATATA TGAAGTGCGT CTCAAATGAT
    TACAGAAGTA TCCAAACACT GACCAGAGAT CACCTGTCCA AGGCGCAGCG AGGGCTTGTG
    GGGGATGGTG TTTCTGAAGT GATGACAAAG CTGAGGGTGA CGTCAAGTGA TGGAACGAGC
    TACAGCGGAG ATCTTTTGGC TATCATAGAT GTGCTAAAAA ACATGACAGA GATATTTAGG
    AGATCCTACT ACAGCCCCAG TAATGCAGAC ATGAGGAATT TTGTGCAGTC TGTGAGTAAC
    TTACTGATGG AGGAAAACAG GGACAGGTGG GAGGAAGCAC AACTGCTGGG ACCCAATGTT
    AAGGAGCTTT TTCGTCTAGT AGAGGACTTT GTGGACGTCA TCAGCCTTCG CATGAAGGAC
    TTCCAGGATA CTTATGAGGT CACGGATAAT CTAGTGTTGA GCATTCACAA GCGGCCCGTG
    GCTGGAAATG CAGATATCAC TTTCCCTATG AAGGGCTGGA GGGGGATGGT GGACTGGGCT
    CGCAGCTCTG AGGACAAAGT CAGAGTGGCC AAAAACATCC TGCTCACTGG AAAACCGGAA
    GAAGATGACG CTTCAGCTTT TGTGACTGGG ATCGTCCTGT ACAGAAACTT AGGCAGTATT
    CTCTCTCTGC AGAGAAATAG CACAGTGCTG AACTCCAAGG TGATCTCTGT GACAATCAAG
    CCCACTCCCA GCTCTCTCTC GAGTCCACTA GAGATTGAGT TCTCACACCT TTACAATGGA
    ACCACAAACC AAACCTGCAT TTCCTGGGAT GAGAACGACA GCTCGTCGTT GCTGGGCTCC
    TGGTCTGCGC GAGGCTGTAG AGCCGTGCCC GTCGACTCCA GCACAACCAA ATGCTTGTGT
    GACAGACTCT CCACCTTCGC CATTTTAGCA CGCCTCAATC CCGATGTGAA CATGGATAAG
    ACCCAGCTTC CATCTGTGAC TCTGATCGTG GGCTGCGGGG TCTCGTCCCT CACCCTGCTC
    CTCCTCATCA TCATCTATGT TTCTGTTTGG AGGTACATCC GCTCAGAGCG CTCTGTCATC
    CTCATCAACT TCTGTCTGTC CATCATCTCC TCCAATGCGC TTATACTCAT CGGTCAAACT
    CAGACACGGA ACAAGGTTGT TTGTACTCTC GTGGCTGCGT TTCTGCACTT TTTCTTCCTG
    TCCTCTTTCT GTTGGGTTCT AACGGAGGCA TGGCAGTCGT ACATGGCTGT GACCGGCCGC
    CTTAGAAACC GTATCATACG CAAGCGTTTC CTTTGCCTTG GTTGGGGTTT ACCTGCTCTC
    GTTGTAGCAA TTTCTGTTGG ATTCACCAAG GCAAAGGGAT ATGGCACTGT TAACTATTGC
    TGGCTGTCTC TGGAAGGAGG GCTGTTGTAC GCATTTGTGG GACCAGCTGC TGCTGTCGTC
    CTGGTTAACA TGGTTATCGG GATTCTGGTT TTTAACAAAC TGGTGTCCAA AGATGGCATT
    ACTGATATGA AACTCAAGGA GAGGGCCGGT CAGATGACAG TGCCACTGTA CAATATGACG
    CTCAAATGTG CCAAGTGCGG AGTTATCTCG TCGCCTGACG TTTCCACCAC AGCTACCAGT
    AACGCCATGG CGTCGCTGTG GAGCTCCTGC GTTGTTCTGC CGTTGCTGGC GCTCACTTGG
    ATGTCGGCAG TCTTGGCTAT TACTGACCGC CGTTCAGCCT TGTTTCAAAT CCTTTTTGCC
    GTCTTTGACT CCCTCGAGGG CTTTGTTATC GTGATGGTGC ATTGCATCCT ACGCAGAGAG
    GTTCAAGAAG CAGTAAAGTG TCGTGTTGTT GACCGTCAAG AAGATGGAAA CGGTGACTCT
    GGTGGATCCT TCCAGAACGG TCATGCTCAG CTGATGACTG ACTTTGAAAA GGATGTGGAC
    ATAGCTTGTC GATCAGGCAC CCTGAAGCGT CCCTCCCAGC AAGGCGAGGA GAAGGTTCCA
    TCCCAGCAGA TCACAGTACA GAAAGGCTCC AACTTCAACA CCCTTCCTGC CAGCATGGCA
    AAGGTCCACC TGCAAAACGT AGCCAACTAC GCCAGTCACA CTCTCACCAT GCGCCGCGAC
    AAAGGCGCCA TCGCCGGGGT CAGCACGGAG TTACCCGGGG CCAAATCTAT TTACATCTGT
    GACGGCGAGC TCTTCAAACA GCTTGACGGA GCACCGGGAG ACATTCCTGA AGGAGGTGGT
    GGAGGTGCAG GTCAGGGTTA TGTGCTTTTG CCCAACAACA ACAACACCCT TAGGCCTACG
    AAGGGTGGCA AGGAAGACCC GGCCGCCAAA TATAACATCA GTATCGAACA GTTACCCCAG
    ACAAGGCTGA TCCACCTGGC CAATCCTGTT GGCACCGATC CCGTGGCAGG CTTCGGGCTG
    AAGAGCTTGC CCACCGACCG AGTCAGCGTT TCATGCTCGG AGAGGGAGTC CCCGGTGCAG
    ACGGTGCAGA ATATCTCCAG CGAGTCCCAG ATGACAAATA CGTGTGAGCA AGGGGATTCT
    GGGAACTCTG GGATGTCTAA GAGCGAGACT GTCTCCACAC TGTCCATGAG CTCTCTGGAG
    AGACGAAAAT CACGCTATGC AGAGCTGGAC TTTGAGAAGA TCATGCACAC ACGGAAGCGT
    CATCAGGACA TGTTTCAGGA CCTGAATCGC AAACTGCAGC ACGCAGAGAA GGACAGAGAC
    TCTCCTCCAG TCGACTGCAA GCCTGGAAAG AGATGGAGCA TTTCGTCAGG AGGAAGCGAC
    AAAACCAACC TTAGTGACAA GCAGCAGACC CCCAGTAAGC GGGCATGGGA GGGCATCAGG
    AAGACTCACT CTCCTCCATC ATGGGTGCGC AAGGAGCTGG AGCCGCTGCA GGCCTCACCG
    CTGGAGATGC AGGCAGTGGA GTGGGAGAAG GCCAGTGCCA CCATCCCTCT AGTGGGCCAG
    GAGATCATGG ACCTGCAGAC GGAGGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290835.1
    CDS747..5396
    Translation

    Target ORF information:

    RefSeq Version XM_009292560.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292560.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCT ACAGGCAGTA TTCCTCAAAA
    GGCCAGGGTT TAAGTCCAGT GAAATACAAA GGAATGCAAG AGGCCATGCT TGGCAAAGGC
    CGGCTTGGCT CTCGTAGCCA ATCCCTGCGC TCGGTGGACA GCCGTAAACG AGAGGACTCT
    GATAAGACCC GCAGCGGACA GCAGTCTTCC CAAACAGAAC ATGGGGCGTG GGGCGAGTGG
    ACACCATGGA GCTTGTGTTC ATCAACATGC GGCCGTGGCT ACAGGGAACG CAACCGCTCC
    TGCAAGCCAC CCCAGTTCGG AGGAAACCCC TGTGATGGTC CAGAGAAACA GACTAAATTC
    TGCAACATCG CTGTATGCCC AGTGGATGGC GTGTGGAACG AGTGGTCTGG TTGGAGTTCC
    TGTTCTTCAA CTTGTTCGAA TGGAACAATG CAGCGAATTC GGGAGTGTAA CGGGCCCTCG
    TATGGGGGCT TAGAGTGTCA AGGCGAGTGG CACGAGACAC GGGATTGCTT CCTTAGAGAA
    TGTCCTGTGG ATGGCCAATG GCAACTTTGG AGCACTTGGT CTGGTTGCAC CAAAACCTGT
    GGTGGAGGAA GTCAGCAGAG ACAGAGGCTG TGTTATGGTC CCTTTTTTGG GGGAGAGGCA
    TGCCCGGGGG ACCGAGAAGA AGTTAGACGT TGCAATGAGA AGCGATGCCC AGAGCCACAT
    GAAATCTGTG ATGAGGAGAA TTTCTCTAAT GTAGTGTGGA AGAAAACACC TGCAGGGGAC
    ACAGCGGCTG TCCGCTGCCC ACCAAATGCT GTAGGATTAA TTCTACGGCG ATGTTCATTA
    GACAATGAGG GTATAGCATA CTGGGAGAAC CCAACATATA TGAAGTGCGT CTCAAATGAT
    TACAGAAGTA TCCAAACACT GACCAGAGAT CACCTGTCCA AGGCGCAGCG AGGGCTTGTG
    GGGGATGGTG TTTCTGAAGT GATGACAAAG CTGAGGGTGA CGTCAAGTGA TGGAACGAGC
    TACAGCGGAG ATCTTTTGGC TATCATAGAT GTGCTAAAAA ACATGACAGA GATATTTAGG
    AGATCCTACT ACAGCCCCAG TAATGCAGAC ATGAGGAATT TTGTGCAGTC TGTGAGTAAC
    TTACTGATGG AGGAAAACAG GGACAGGTGG GAGGAAGCAC AACTGCTGGG ACCCAATGTT
    AAGGAGCTTT TTCGTCTAGT AGAGGACTTT GTGGACGTCA TCAGCCTTCG CATGAAGGAC
    TTCCAGGATA CTTATGAGGT CACGGATAAT CTAGTGTTGA GCATTCACAA GCGGCCCGTG
    GCTGGAAATG CAGATATCAC TTTCCCTATG AAGGGCTGGA GGGGGATGGT GGACTGGGCT
    CGCAGCTCTG AGGACAAAGT CAGAGTGGCC AAAAACATCC TGCTCACTGG AAAACCGGAA
    GAAGATGACG CTTCAGCTTT TGTGACTGGG ATCGTCCTGT ACAGAAACTT AGGCAGTATT
    CTCTCTCTGC AGAGAAATAG CACAGTGCTG AACTCCAAGG TGATCTCTGT GACAATCAAG
    CCCACTCCCA GCTCTCTCTC GAGTCCACTA GAGATTGAGT TCTCACACCT TTACAATGGA
    ACCACAAACC AAACCTGCAT TTCCTGGGAT GAGAACGACA GCTCGTCGTT GCTGGGCTCC
    TGGTCTGCGC GAGGCTGTAG AGCCGTGCCC GTCGACTCCA GCACAACCAA ATGCTTGTGT
    GACAGACTCT CCACCTTCGC CATTTTAGCA CGCCTCAATC CCGATGTGAA CATGGATAAG
    ACCCAGCTTC CATCTGTGAC TCTGATCGTG GGCTGCGGGG TCTCGTCCCT CACCCTGCTC
    CTCCTCATCA TCATCTATGT TTCTGTTTGG AGGTACATCC GCTCAGAGCG CTCTGTCATC
    CTCATCAACT TCTGTCTGTC CATCATCTCC TCCAATGCGC TTATACTCAT CGGTCAAACT
    CAGACACGGA ACAAGGTTGT TTGTACTCTC GTGGCTGCGT TTCTGCACTT TTTCTTCCTG
    TCCTCTTTCT GTTGGGTTCT AACGGAGGCA TGGCAGTCGT ACATGGCTGT GACCGGCCGC
    CTTAGAAACC GTATCATACG CAAGCGTTTC CTTTGCCTTG GTTGGGGTTT ACCTGCTCTC
    GTTGTAGCAA TTTCTGTTGG ATTCACCAAG GCAAAGGGAT ATGGCACTGT TAACTATTGC
    TGGCTGTCTC TGGAAGGAGG GCTGTTGTAC GCATTTGTGG GACCAGCTGC TGCTGTCGTC
    CTGGTTAACA TGGTTATCGG GATTCTGGTT TTTAACAAAC TGGTGTCCAA AGATGGCATT
    ACTGATATGA AACTCAAGGA GAGGGCCGGT CAGATGACAG TGCCACTGTA CAATATGACG
    CTCAAATGTG CCAAGTGCGG AGTTATCTCG TCGCCTGACG TTTCCACCAC AGCTACCAGT
    AACGCCATGG CGTCGCTGTG GAGCTCCTGC GTTGTTCTGC CGTTGCTGGC GCTCACTTGG
    ATGTCGGCAG TCTTGGCTAT TACTGACCGC CGTTCAGCCT TGTTTCAAAT CCTTTTTGCC
    GTCTTTGACT CCCTCGAGGG CTTTGTTATC GTGATGGTGC ATTGCATCCT ACGCAGAGAG
    GTTCAAGAAG CAGTAAAGTG TCGTGTTGTT GACCGTCAAG AAGATGGAAA CGGTGACTCT
    GGTGGATCCT TCCAGAACGG TCATGCTCAG CTGATGACTG ACTTTGAAAA GGATGTGGAC
    ATAGCTTGTC GATCAGGCAC CCTGAAGCGT CCCTCCCAGC AAGGCGAGGA GAAGGTTCCA
    TCCCAGCAGA TCACAGTACA GAAAGGCTCC AACTTCAACA CCCTTCCTGC CAGCATGGCA
    AAGGTCCACC TGCAAAACGT AGCCAACTAC GCCAGTCACA CTCTCACCAT GCGCCGCGAC
    AAAGGCGCCA TCGCCGGGGT CAGCACGGAG TTACCCGGGG CCAAATCTAT TTACATCTGT
    GACGGCGAGC TCTTCAAACA GCTTGACGGA GCACCGGGAG ACATTCCTGA AGGAGGTGGT
    GGAGGTGCAG GTCAGGGTTA TGTGCTTTTG CCCAACAACA ACAACACCCT TAGGCCTACG
    AAGGGTGGCA AGGAAGACCC GGCCGCCAAA TATAACATCA GTATCGAACA GTTACCCCAG
    ACAAGGCTGA TCCACCTGGC CAATCCTGTT GGCACCGATC CCGTGGCAGG CTTCGGGCTG
    AAGAGCTTGC CCACCGACCG AGTCAGCGTT TCATGCTCGG AGAGGGAGTC CCCGGTGCAG
    ACGGTGCAGA ATATCTCCAG CGAGTCCCAG ATGACAAATA CGTGTGAGCA AGGGGATTCT
    GGGAACTCTG GGATGTCTAA GAGCGAGACT GTCTCCACAC TGTCCATGAG CTCTCTGGAG
    AGACGAAAAT CACGCTATGC AGAGCTGGAC TTTGAGAAGA TCATGCACAC ACGGAAGCGT
    CATCAGGACA TGTTTCAGGA CCTGAATCGC AAACTGCAGC ACGCAGAGAA GGACAGAGAC
    TCTCCTCCAG TCGACTGCAA GCCTGGAAAG AGATGGAGCA TTTCGTCAGG AGGAAGCGAC
    AAAACCAACC TTAGTGACAA GCAGCAGACC CCCAGTAAGC GGGCATGGGA GGGCATCAGG
    AAGACTCACT CTCCTCCATC ATGGGTGCGC AAGGAGCTGG AGCCGCTGCA GGCCTCACCG
    CTGGAGATGC AGGCAGTGGA GTGGGAGAAG GCCAGTGCCA CCATCCCTCT AGTGGGCCAG
    GAGATCATGG ACCTGCAGAC GGAGGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44102
    Clone ID Related Accession (Same CDS sequence) XM_009292560.3 , XM_009292560.2
    Accession Version XM_009292560.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4650bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product brain-specific angiogenesis inhibitor 1 isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292560.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCT ACAGGCAGTA TTCCTCAAAA
    GGCCAGGGTT TAAGTCCAGT GAAATACAAA GGAATGCAAG AGGCCATGCT TGGCAAAGGC
    CGGCTTGGCT CTCGTAGCCA ATCCCTGCGC TCGGTGGACA GCCGTAAACG AGAGGACTCT
    GATAAGACCC GCAGCGGACA GCAGTCTTCC CAAACAGAAC ATGGGGCGTG GGGCGAGTGG
    ACACCATGGA GCTTGTGTTC ATCAACATGC GGCCGTGGCT ACAGGGAACG CAACCGCTCC
    TGCAAGCCAC CCCAGTTCGG AGGAAACCCC TGTGATGGTC CAGAGAAACA GACTAAATTC
    TGCAACATCG CTGTATGCCC AGTGGATGGC GTGTGGAACG AGTGGTCTGG TTGGAGTTCC
    TGTTCTTCAA CTTGTTCGAA TGGAACAATG CAGCGAATTC GGGAGTGTAA CGGGCCCTCG
    TATGGGGGCT TAGAGTGTCA AGGCGAGTGG CACGAGACAC GGGATTGCTT CCTTAGAGAA
    TGTCCTGTGG ATGGCCAATG GCAACTTTGG AGCACTTGGT CTGGTTGCAC CAAAACCTGT
    GGTGGAGGAA GTCAGCAGAG ACAGAGGCTG TGTTATGGTC CCTTTTTTGG GGGAGAGGCA
    TGCCCGGGGG ACCGAGAAGA AGTTAGACGT TGCAATGAGA AGCGATGCCC AGAGCCACAT
    GAAATCTGTG ATGAGGAGAA TTTCTCTAAT GTAGTGTGGA AGAAAACACC TGCAGGGGAC
    ACAGCGGCTG TCCGCTGCCC ACCAAATGCT GTAGGATTAA TTCTACGGCG ATGTTCATTA
    GACAATGAGG GTATAGCATA CTGGGAGAAC CCAACATATA TGAAGTGCGT CTCAAATGAT
    TACAGAAGTA TCCAAACACT GACCAGAGAT CACCTGTCCA AGGCGCAGCG AGGGCTTGTG
    GGGGATGGTG TTTCTGAAGT GATGACAAAG CTGAGGGTGA CGTCAAGTGA TGGAACGAGC
    TACAGCGGAG ATCTTTTGGC TATCATAGAT GTGCTAAAAA ACATGACAGA GATATTTAGG
    AGATCCTACT ACAGCCCCAG TAATGCAGAC ATGAGGAATT TTGTGCAGTC TGTGAGTAAC
    TTACTGATGG AGGAAAACAG GGACAGGTGG GAGGAAGCAC AACTGCTGGG ACCCAATGTT
    AAGGAGCTTT TTCGTCTAGT AGAGGACTTT GTGGACGTCA TCAGCCTTCG CATGAAGGAC
    TTCCAGGATA CTTATGAGGT CACGGATAAT CTAGTGTTGA GCATTCACAA GCGGCCCGTG
    GCTGGAAATG CAGATATCAC TTTCCCTATG AAGGGCTGGA GGGGGATGGT GGACTGGGCT
    CGCAGCTCTG AGGACAAAGT CAGAGTGGCC AAAAACATCC TGCTCACTGG AAAACCGGAA
    GAAGATGACG CTTCAGCTTT TGTGACTGGG ATCGTCCTGT ACAGAAACTT AGGCAGTATT
    CTCTCTCTGC AGAGAAATAG CACAGTGCTG AACTCCAAGG TGATCTCTGT GACAATCAAG
    CCCACTCCCA GCTCTCTCTC GAGTCCACTA GAGATTGAGT TCTCACACCT TTACAATGGA
    ACCACAAACC AAACCTGCAT TTCCTGGGAT GAGAACGACA GCTCGTCGTT GCTGGGCTCC
    TGGTCTGCGC GAGGCTGTAG AGCCGTGCCC GTCGACTCCA GCACAACCAA ATGCTTGTGT
    GACAGACTCT CCACCTTCGC CATTTTAGCA CGCCTCAATC CCGATGTGAA CATGGATAAG
    ACCCAGCTTC CATCTGTGAC TCTGATCGTG GGCTGCGGGG TCTCGTCCCT CACCCTGCTC
    CTCCTCATCA TCATCTATGT TTCTGTTTGG AGGTACATCC GCTCAGAGCG CTCTGTCATC
    CTCATCAACT TCTGTCTGTC CATCATCTCC TCCAATGCGC TTATACTCAT CGGTCAAACT
    CAGACACGGA ACAAGGTTGT TTGTACTCTC GTGGCTGCGT TTCTGCACTT TTTCTTCCTG
    TCCTCTTTCT GTTGGGTTCT AACGGAGGCA TGGCAGTCGT ACATGGCTGT GACCGGCCGC
    CTTAGAAACC GTATCATACG CAAGCGTTTC CTTTGCCTTG GTTGGGGTTT ACCTGCTCTC
    GTTGTAGCAA TTTCTGTTGG ATTCACCAAG GCAAAGGGAT ATGGCACTGT TAACTATTGC
    TGGCTGTCTC TGGAAGGAGG GCTGTTGTAC GCATTTGTGG GACCAGCTGC TGCTGTCGTC
    CTGGTTAACA TGGTTATCGG GATTCTGGTT TTTAACAAAC TGGTGTCCAA AGATGGCATT
    ACTGATATGA AACTCAAGGA GAGGGCCGGT CAGATGACAG TGCCACTGTA CAATATGACG
    CTCAAATGTG CCAAGTGCGG AGTTATCTCG TCGCCTGACG TTTCCACCAC AGCTACCAGT
    AACGCCATGG CGTCGCTGTG GAGCTCCTGC GTTGTTCTGC CGTTGCTGGC GCTCACTTGG
    ATGTCGGCAG TCTTGGCTAT TACTGACCGC CGTTCAGCCT TGTTTCAAAT CCTTTTTGCC
    GTCTTTGACT CCCTCGAGGG CTTTGTTATC GTGATGGTGC ATTGCATCCT ACGCAGAGAG
    GTTCAAGAAG CAGTAAAGTG TCGTGTTGTT GACCGTCAAG AAGATGGAAA CGGTGACTCT
    GGTGGATCCT TCCAGAACGG TCATGCTCAG CTGATGACTG ACTTTGAAAA GGATGTGGAC
    ATAGCTTGTC GATCAGGCAC CCTGAAGCGT CCCTCCCAGC AAGGCGAGGA GAAGGTTCCA
    TCCCAGCAGA TCACAGTACA GAAAGGCTCC AACTTCAACA CCCTTCCTGC CAGCATGGCA
    AAGGTCCACC TGCAAAACGT AGCCAACTAC GCCAGTCACA CTCTCACCAT GCGCCGCGAC
    AAAGGCGCCA TCGCCGGGGT CAGCACGGAG TTACCCGGGG CCAAATCTAT TTACATCTGT
    GACGGCGAGC TCTTCAAACA GCTTGACGGA GCACCGGGAG ACATTCCTGA AGGAGGTGGT
    GGAGGTGCAG GTCAGGGTTA TGTGCTTTTG CCCAACAACA ACAACACCCT TAGGCCTACG
    AAGGGTGGCA AGGAAGACCC GGCCGCCAAA TATAACATCA GTATCGAACA GTTACCCCAG
    ACAAGGCTGA TCCACCTGGC CAATCCTGTT GGCACCGATC CCGTGGCAGG CTTCGGGCTG
    AAGAGCTTGC CCACCGACCG AGTCAGCGTT TCATGCTCGG AGAGGGAGTC CCCGGTGCAG
    ACGGTGCAGA ATATCTCCAG CGAGTCCCAG ATGACAAATA CGTGTGAGCA AGGGGATTCT
    GGGAACTCTG GGATGTCTAA GAGCGAGACT GTCTCCACAC TGTCCATGAG CTCTCTGGAG
    AGACGAAAAT CACGCTATGC AGAGCTGGAC TTTGAGAAGA TCATGCACAC ACGGAAGCGT
    CATCAGGACA TGTTTCAGGA CCTGAATCGC AAACTGCAGC ACGCAGAGAA GGACAGAGAC
    TCTCCTCCAG TCGACTGCAA GCCTGGAAAG AGATGGAGCA TTTCGTCAGG AGGAAGCGAC
    AAAACCAACC TTAGTGACAA GCAGCAGACC CCCAGTAAGC GGGCATGGGA GGGCATCAGG
    AAGACTCACT CTCCTCCATC ATGGGTGCGC AAGGAGCTGG AGCCGCTGCA GGCCTCACCG
    CTGGAGATGC AGGCAGTGGA GTGGGAGAAG GCCAGTGCCA CCATCCCTCT AGTGGGCCAG
    GAGATCATGG ACCTGCAGAC GGAGGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290835.1
    CDS731..5380
    Translation

    Target ORF information:

    RefSeq Version XM_009292560.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292560.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCT ACAGGCAGTA TTCCTCAAAA
    GGCCAGGGTT TAAGTCCAGT GAAATACAAA GGAATGCAAG AGGCCATGCT TGGCAAAGGC
    CGGCTTGGCT CTCGTAGCCA ATCCCTGCGC TCGGTGGACA GCCGTAAACG AGAGGACTCT
    GATAAGACCC GCAGCGGACA GCAGTCTTCC CAAACAGAAC ATGGGGCGTG GGGCGAGTGG
    ACACCATGGA GCTTGTGTTC ATCAACATGC GGCCGTGGCT ACAGGGAACG CAACCGCTCC
    TGCAAGCCAC CCCAGTTCGG AGGAAACCCC TGTGATGGTC CAGAGAAACA GACTAAATTC
    TGCAACATCG CTGTATGCCC AGTGGATGGC GTGTGGAACG AGTGGTCTGG TTGGAGTTCC
    TGTTCTTCAA CTTGTTCGAA TGGAACAATG CAGCGAATTC GGGAGTGTAA CGGGCCCTCG
    TATGGGGGCT TAGAGTGTCA AGGCGAGTGG CACGAGACAC GGGATTGCTT CCTTAGAGAA
    TGTCCTGTGG ATGGCCAATG GCAACTTTGG AGCACTTGGT CTGGTTGCAC CAAAACCTGT
    GGTGGAGGAA GTCAGCAGAG ACAGAGGCTG TGTTATGGTC CCTTTTTTGG GGGAGAGGCA
    TGCCCGGGGG ACCGAGAAGA AGTTAGACGT TGCAATGAGA AGCGATGCCC AGAGCCACAT
    GAAATCTGTG ATGAGGAGAA TTTCTCTAAT GTAGTGTGGA AGAAAACACC TGCAGGGGAC
    ACAGCGGCTG TCCGCTGCCC ACCAAATGCT GTAGGATTAA TTCTACGGCG ATGTTCATTA
    GACAATGAGG GTATAGCATA CTGGGAGAAC CCAACATATA TGAAGTGCGT CTCAAATGAT
    TACAGAAGTA TCCAAACACT GACCAGAGAT CACCTGTCCA AGGCGCAGCG AGGGCTTGTG
    GGGGATGGTG TTTCTGAAGT GATGACAAAG CTGAGGGTGA CGTCAAGTGA TGGAACGAGC
    TACAGCGGAG ATCTTTTGGC TATCATAGAT GTGCTAAAAA ACATGACAGA GATATTTAGG
    AGATCCTACT ACAGCCCCAG TAATGCAGAC ATGAGGAATT TTGTGCAGTC TGTGAGTAAC
    TTACTGATGG AGGAAAACAG GGACAGGTGG GAGGAAGCAC AACTGCTGGG ACCCAATGTT
    AAGGAGCTTT TTCGTCTAGT AGAGGACTTT GTGGACGTCA TCAGCCTTCG CATGAAGGAC
    TTCCAGGATA CTTATGAGGT CACGGATAAT CTAGTGTTGA GCATTCACAA GCGGCCCGTG
    GCTGGAAATG CAGATATCAC TTTCCCTATG AAGGGCTGGA GGGGGATGGT GGACTGGGCT
    CGCAGCTCTG AGGACAAAGT CAGAGTGGCC AAAAACATCC TGCTCACTGG AAAACCGGAA
    GAAGATGACG CTTCAGCTTT TGTGACTGGG ATCGTCCTGT ACAGAAACTT AGGCAGTATT
    CTCTCTCTGC AGAGAAATAG CACAGTGCTG AACTCCAAGG TGATCTCTGT GACAATCAAG
    CCCACTCCCA GCTCTCTCTC GAGTCCACTA GAGATTGAGT TCTCACACCT TTACAATGGA
    ACCACAAACC AAACCTGCAT TTCCTGGGAT GAGAACGACA GCTCGTCGTT GCTGGGCTCC
    TGGTCTGCGC GAGGCTGTAG AGCCGTGCCC GTCGACTCCA GCACAACCAA ATGCTTGTGT
    GACAGACTCT CCACCTTCGC CATTTTAGCA CGCCTCAATC CCGATGTGAA CATGGATAAG
    ACCCAGCTTC CATCTGTGAC TCTGATCGTG GGCTGCGGGG TCTCGTCCCT CACCCTGCTC
    CTCCTCATCA TCATCTATGT TTCTGTTTGG AGGTACATCC GCTCAGAGCG CTCTGTCATC
    CTCATCAACT TCTGTCTGTC CATCATCTCC TCCAATGCGC TTATACTCAT CGGTCAAACT
    CAGACACGGA ACAAGGTTGT TTGTACTCTC GTGGCTGCGT TTCTGCACTT TTTCTTCCTG
    TCCTCTTTCT GTTGGGTTCT AACGGAGGCA TGGCAGTCGT ACATGGCTGT GACCGGCCGC
    CTTAGAAACC GTATCATACG CAAGCGTTTC CTTTGCCTTG GTTGGGGTTT ACCTGCTCTC
    GTTGTAGCAA TTTCTGTTGG ATTCACCAAG GCAAAGGGAT ATGGCACTGT TAACTATTGC
    TGGCTGTCTC TGGAAGGAGG GCTGTTGTAC GCATTTGTGG GACCAGCTGC TGCTGTCGTC
    CTGGTTAACA TGGTTATCGG GATTCTGGTT TTTAACAAAC TGGTGTCCAA AGATGGCATT
    ACTGATATGA AACTCAAGGA GAGGGCCGGT CAGATGACAG TGCCACTGTA CAATATGACG
    CTCAAATGTG CCAAGTGCGG AGTTATCTCG TCGCCTGACG TTTCCACCAC AGCTACCAGT
    AACGCCATGG CGTCGCTGTG GAGCTCCTGC GTTGTTCTGC CGTTGCTGGC GCTCACTTGG
    ATGTCGGCAG TCTTGGCTAT TACTGACCGC CGTTCAGCCT TGTTTCAAAT CCTTTTTGCC
    GTCTTTGACT CCCTCGAGGG CTTTGTTATC GTGATGGTGC ATTGCATCCT ACGCAGAGAG
    GTTCAAGAAG CAGTAAAGTG TCGTGTTGTT GACCGTCAAG AAGATGGAAA CGGTGACTCT
    GGTGGATCCT TCCAGAACGG TCATGCTCAG CTGATGACTG ACTTTGAAAA GGATGTGGAC
    ATAGCTTGTC GATCAGGCAC CCTGAAGCGT CCCTCCCAGC AAGGCGAGGA GAAGGTTCCA
    TCCCAGCAGA TCACAGTACA GAAAGGCTCC AACTTCAACA CCCTTCCTGC CAGCATGGCA
    AAGGTCCACC TGCAAAACGT AGCCAACTAC GCCAGTCACA CTCTCACCAT GCGCCGCGAC
    AAAGGCGCCA TCGCCGGGGT CAGCACGGAG TTACCCGGGG CCAAATCTAT TTACATCTGT
    GACGGCGAGC TCTTCAAACA GCTTGACGGA GCACCGGGAG ACATTCCTGA AGGAGGTGGT
    GGAGGTGCAG GTCAGGGTTA TGTGCTTTTG CCCAACAACA ACAACACCCT TAGGCCTACG
    AAGGGTGGCA AGGAAGACCC GGCCGCCAAA TATAACATCA GTATCGAACA GTTACCCCAG
    ACAAGGCTGA TCCACCTGGC CAATCCTGTT GGCACCGATC CCGTGGCAGG CTTCGGGCTG
    AAGAGCTTGC CCACCGACCG AGTCAGCGTT TCATGCTCGG AGAGGGAGTC CCCGGTGCAG
    ACGGTGCAGA ATATCTCCAG CGAGTCCCAG ATGACAAATA CGTGTGAGCA AGGGGATTCT
    GGGAACTCTG GGATGTCTAA GAGCGAGACT GTCTCCACAC TGTCCATGAG CTCTCTGGAG
    AGACGAAAAT CACGCTATGC AGAGCTGGAC TTTGAGAAGA TCATGCACAC ACGGAAGCGT
    CATCAGGACA TGTTTCAGGA CCTGAATCGC AAACTGCAGC ACGCAGAGAA GGACAGAGAC
    TCTCCTCCAG TCGACTGCAA GCCTGGAAAG AGATGGAGCA TTTCGTCAGG AGGAAGCGAC
    AAAACCAACC TTAGTGACAA GCAGCAGACC CCCAGTAAGC GGGCATGGGA GGGCATCAGG
    AAGACTCACT CTCCTCCATC ATGGGTGCGC AAGGAGCTGG AGCCGCTGCA GGCCTCACCG
    CTGGAGATGC AGGCAGTGGA GTGGGAGAAG GCCAGTGCCA CCATCCCTCT AGTGGGCCAG
    GAGATCATGG ACCTGCAGAC GGAGGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44104
    Clone ID Related Accession (Same CDS sequence) XM_009292562.2 , XM_009292562.3
    Accession Version XM_009292562.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4644bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product brain-specific angiogenesis inhibitor 1 isoform X10
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292562.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCA AAGGCCGGCT TGGCTCTCGT
    AGCCAATCCC TGCGCTCGGT GGACAGCCGT AAACGAGAGG ACTCTGATAA GACCCGCAGC
    GGACAGCAGT CTTCCCAAAC AGTGGATTCT CCGTCGGGTG AGGAGTGGTC TGCGTGGAGC
    GTGTGTTCGG CCACGTGTGG TGAGGGCTGG CAGAGCCGCA CACGCTTCTG CGTCTCCGTG
    TCGTACAGCA CGCAGTGCAG CGGGCCACTG CGCGAACAGA GACCCTGCAA TAACACTGCT
    GTGTGTCCAG AACATGGGGC GTGGGGCGAG TGGACACCAT GGAGCTTGTG TTCATCAACA
    TGCGGCCGTG GCTACAGGGA ACGCAACCGC TCCTGCAAGC CACCCCAGTT CGGAGGAAAC
    CCCTGTGATG GTCCAGAGAA ACAGACTAAA TTCTGCAACA TCGCTGTATG CCCAGTGGAT
    GGCGTGTGGA ACGAGTGGTC TGGTTGGAGT TCCTGTTCTT CAACTTGTTC GAATGGAACA
    ATGCAGCGAA TTCGGGAGTG TAACGGGCCC TCGTATGGGG GCTTAGAGTG TCAAGGCGAG
    TGGCACGAGA CACGGGATTG CTTCCTTAGA GAATGTCCTG TGGATGGCCA ATGGCAACTT
    TGGAGCACTT GGTCTGGTTG CACCAAAACC TGTGGTGGAG GAAGTCAGCA GAGACAGAGG
    CTGTGTTATG GTCCCTTTTT TGGGGGAGAG GCATGCCCGG GGGACCGAGA AGAAGTTAGA
    CGTTGCAATG AGAAGCGATG CCCAGAGCCA CATGAAATCT GTGATGAGGA GAATTTCTCT
    AATGTAGTGT GGAAGAAAAC ACCTGCAGGG GACACAGCGG CTGTCCGCTG CCCACCAAAT
    GCTGTAGGAT TAATTCTACG GCGATGTTCA TTAGACAATG AGGGTATAGC ATACTGGGAG
    AACCCAACAT ATATGAAGTG CGTCTCAAAT GATTACAGAA GTATCCAAAC ACTGACCAGA
    GATCACCTGT CCAAGGCGCA GCGAGGGCTT GTGGGGGATG GTGTTTCTGA AGTGATGACA
    AAGCTGAGGG TGACGTCAAG TGATGGAACG AGCTACAGCG GAGATCTTTT GGCTATCATA
    GATGTGCTAA AAAACATGAC AGAGATATTT AGGAGATCCT ACTACAGCCC CAGTAATGCA
    GACATGAGGA ATTTTGTGCA GTCTGTGAGT AACTTACTGA TGGAGGAAAA CAGGGACAGG
    TGGGAGGAAG CACAACTGCT GGGACCCAAT GTTAAGGAGC TTTTTCGTCT AGTAGAGGAC
    TTTGTGGACG TCATCAGCCT TCGCATGAAG GACTTCCAGG ATACTTATGA GGTCACGGAT
    AATCTAGTGT TGAGCATTCA CAAGCGGCCC GTGGCTGGAA ATGCAGATAT CACTTTCCCT
    ATGAAGGGCT GGAGGGGGAT GGTGGACTGG GCTCGCAGCT CTGAGGACAA AGTCAGAGTG
    GCCAAAAACA TCCTGCTCAC TGGAAAACCG GAAGAAGATG ACGCTTCAGC TTTTGTGACT
    GGGATCGTCC TGTACAGAAA CTTAGGCAGT ATTCTCTCTC TGCAGAGAAA TAGCACAGTG
    CTGAACTCCA AGGTGATCTC TGTGACAATC AAGCCCACTC CCAGCTCTCT CTCGAGTCCA
    CTAGAGATTG AGTTCTCACA CCTTTACAAT GGAACCACAA ACCAAACCTG CATTTCCTGG
    GATGAGAACG ACAGCTCGTC GTTGCTGGGC TCCTGGTCTG CGCGAGGCTG TAGAGCCGTG
    CCCGTCGACT CCAGCACAAC CAAATGCTTG TGTGACAGAC TCTCCACCTT CGCCATTTTA
    GCACGCCTCA ATCCCGATGT GAACATGGAT AAGACCCAGC TTCCATCTGT GACTCTGATC
    GTGGGCTGCG GGGTCTCGTC CCTCACCCTG CTCCTCCTCA TCATCATCTA TGTTTCTGTT
    TGGAGGTACA TCCGCTCAGA GCGCTCTGTC ATCCTCATCA ACTTCTGTCT GTCCATCATC
    TCCTCCAATG CGCTTATACT CATCGGTCAA ACTCAGACAC GGAACAAGGT TGTTTGTACT
    CTCGTGGCTG CGTTTCTGCA CTTTTTCTTC CTGTCCTCTT TCTGTTGGGT TCTAACGGAG
    GCATGGCAGT CGTACATGGC TGTGACCGGC CGCCTTAGAA ACCGTATCAT ACGCAAGCGT
    TTCCTTTGCC TTGGTTGGGG TTTACCTGCT CTCGTTGTAG CAATTTCTGT TGGATTCACC
    AAGGCAAAGG GATATGGCAC TGTTAACTAT TGCTGGCTGT CTCTGGAAGG AGGGCTGTTG
    TACGCATTTG TGGGACCAGC TGCTGCTGTC GTCCTGGTTA ACATGGTTAT CGGGATTCTG
    GTTTTTAACA AACTGGTGTC CAAAGATGGC ATTACTGATA TGAAACTCAA GGAGAGGGCC
    GGGGCGTCGC TGTGGAGCTC CTGCGTTGTT CTGCCGTTGC TGGCGCTCAC TTGGATGTCG
    GCAGTCTTGG CTATTACTGA CCGCCGTTCA GCCTTGTTTC AAATCCTTTT TGCCGTCTTT
    GACTCCCTCG AGGGCTTTGT TATCGTGATG GTGCATTGCA TCCTACGCAG AGAGGTTCAA
    GAAGCAGTAA AGTGTCGTGT TGTTGACCGT CAAGAAGATG GAAACGGTGA CTCTGGTGGA
    TCCTTCCAGA ACGGTCATGC TCAGCTGATG ACTGACTTTG AAAAGGATGT GGACATAGCT
    TGTCGATCAG GCACCCTGAA GCGTCCCTCC CAGCAAGGCG AGGAGAAGGT TCCATCCCAG
    CAGATCACAG TACAGAAAGG CTCCAACTTC AACACCCTTC CTGCCAGCAT GGCAAAGGTC
    CACCTGCAAA ACGTAGCCAA CTACGCCAGT CACACTCTCA CCATGCGCCG CGACAAAGGC
    GCCATCGCCG GGGTCAGCAC GGAGTTACCC GGGGCCAAAT CTATTTACAT CTGTGACGGC
    GAGCTCTTCA AACAGCTTGA CGGAGCACCG GGAGACATTC CTGAAGGAGG TGGTGGAGGT
    GCAGGTCAGG GTTATGTGCT TTTGCCCAAC AACAACAACA CCCTTAGGCC TACGAAGGGT
    GGCAAGGAAG ACCCGGCCGC CAAATATAAC ATCAGTATCG AACAGTTACC CCAGACAAGG
    CTGATCCACC TGGCCAATCC TGTTGGCACC GATCCCGTGG CAGGCTTCGG GCTGAAGAGC
    TTGCCCACCG ACCGAGTCAG CGTTTCATGC TCGGAGAGGG AGTCCCCGGT GCAGACGGTG
    CAGAATATCT CCAGCGAGTC CCAGATGACA AATACGTGTG AGCAAGGGGA TTCTGGGAAC
    TCTGGGATGT CTAAGAGCGA GACTGTCTCC ACACTGTCCA TGAGCTCTCT GGAGAGACGA
    AAATCACGCT ATGCAGAGCT GGACTTTGAG AAGATCATGC ACACACGGAA GCGTCATCAG
    GACATGTTTC AGGACCTGAA TCGCAAACTG CAGCACGCAG AGAAGGACAG AGACTCTCCT
    CCAGTCGACT GCAAGCCTGG AAAGAGATGG AGCATTTCGT CAGGAGGAAG CGACAAAACC
    AACCTTAGTG ACAAGCAGCA GACCCCCAGT AAGCGGGCAT GGGAGGGCAT CAGGAAGACT
    CACTCTCCTC CATCATGGGT GCGCAAGGAG CTGGAGCCGC TGCAGGCCTC ACCGCTGGAG
    ATGCAGGCAG TGGAGTGGGA GAAGGCCAGT GCCACCATCC CTCTAGTGGG CCAGGAGATC
    ATGGACCTGC AGACGGAGGT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290837.1
    CDS731..5374
    Translation

    Target ORF information:

    RefSeq Version XM_009292562.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292562.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCA AAGGCCGGCT TGGCTCTCGT
    AGCCAATCCC TGCGCTCGGT GGACAGCCGT AAACGAGAGG ACTCTGATAA GACCCGCAGC
    GGACAGCAGT CTTCCCAAAC AGTGGATTCT CCGTCGGGTG AGGAGTGGTC TGCGTGGAGC
    GTGTGTTCGG CCACGTGTGG TGAGGGCTGG CAGAGCCGCA CACGCTTCTG CGTCTCCGTG
    TCGTACAGCA CGCAGTGCAG CGGGCCACTG CGCGAACAGA GACCCTGCAA TAACACTGCT
    GTGTGTCCAG AACATGGGGC GTGGGGCGAG TGGACACCAT GGAGCTTGTG TTCATCAACA
    TGCGGCCGTG GCTACAGGGA ACGCAACCGC TCCTGCAAGC CACCCCAGTT CGGAGGAAAC
    CCCTGTGATG GTCCAGAGAA ACAGACTAAA TTCTGCAACA TCGCTGTATG CCCAGTGGAT
    GGCGTGTGGA ACGAGTGGTC TGGTTGGAGT TCCTGTTCTT CAACTTGTTC GAATGGAACA
    ATGCAGCGAA TTCGGGAGTG TAACGGGCCC TCGTATGGGG GCTTAGAGTG TCAAGGCGAG
    TGGCACGAGA CACGGGATTG CTTCCTTAGA GAATGTCCTG TGGATGGCCA ATGGCAACTT
    TGGAGCACTT GGTCTGGTTG CACCAAAACC TGTGGTGGAG GAAGTCAGCA GAGACAGAGG
    CTGTGTTATG GTCCCTTTTT TGGGGGAGAG GCATGCCCGG GGGACCGAGA AGAAGTTAGA
    CGTTGCAATG AGAAGCGATG CCCAGAGCCA CATGAAATCT GTGATGAGGA GAATTTCTCT
    AATGTAGTGT GGAAGAAAAC ACCTGCAGGG GACACAGCGG CTGTCCGCTG CCCACCAAAT
    GCTGTAGGAT TAATTCTACG GCGATGTTCA TTAGACAATG AGGGTATAGC ATACTGGGAG
    AACCCAACAT ATATGAAGTG CGTCTCAAAT GATTACAGAA GTATCCAAAC ACTGACCAGA
    GATCACCTGT CCAAGGCGCA GCGAGGGCTT GTGGGGGATG GTGTTTCTGA AGTGATGACA
    AAGCTGAGGG TGACGTCAAG TGATGGAACG AGCTACAGCG GAGATCTTTT GGCTATCATA
    GATGTGCTAA AAAACATGAC AGAGATATTT AGGAGATCCT ACTACAGCCC CAGTAATGCA
    GACATGAGGA ATTTTGTGCA GTCTGTGAGT AACTTACTGA TGGAGGAAAA CAGGGACAGG
    TGGGAGGAAG CACAACTGCT GGGACCCAAT GTTAAGGAGC TTTTTCGTCT AGTAGAGGAC
    TTTGTGGACG TCATCAGCCT TCGCATGAAG GACTTCCAGG ATACTTATGA GGTCACGGAT
    AATCTAGTGT TGAGCATTCA CAAGCGGCCC GTGGCTGGAA ATGCAGATAT CACTTTCCCT
    ATGAAGGGCT GGAGGGGGAT GGTGGACTGG GCTCGCAGCT CTGAGGACAA AGTCAGAGTG
    GCCAAAAACA TCCTGCTCAC TGGAAAACCG GAAGAAGATG ACGCTTCAGC TTTTGTGACT
    GGGATCGTCC TGTACAGAAA CTTAGGCAGT ATTCTCTCTC TGCAGAGAAA TAGCACAGTG
    CTGAACTCCA AGGTGATCTC TGTGACAATC AAGCCCACTC CCAGCTCTCT CTCGAGTCCA
    CTAGAGATTG AGTTCTCACA CCTTTACAAT GGAACCACAA ACCAAACCTG CATTTCCTGG
    GATGAGAACG ACAGCTCGTC GTTGCTGGGC TCCTGGTCTG CGCGAGGCTG TAGAGCCGTG
    CCCGTCGACT CCAGCACAAC CAAATGCTTG TGTGACAGAC TCTCCACCTT CGCCATTTTA
    GCACGCCTCA ATCCCGATGT GAACATGGAT AAGACCCAGC TTCCATCTGT GACTCTGATC
    GTGGGCTGCG GGGTCTCGTC CCTCACCCTG CTCCTCCTCA TCATCATCTA TGTTTCTGTT
    TGGAGGTACA TCCGCTCAGA GCGCTCTGTC ATCCTCATCA ACTTCTGTCT GTCCATCATC
    TCCTCCAATG CGCTTATACT CATCGGTCAA ACTCAGACAC GGAACAAGGT TGTTTGTACT
    CTCGTGGCTG CGTTTCTGCA CTTTTTCTTC CTGTCCTCTT TCTGTTGGGT TCTAACGGAG
    GCATGGCAGT CGTACATGGC TGTGACCGGC CGCCTTAGAA ACCGTATCAT ACGCAAGCGT
    TTCCTTTGCC TTGGTTGGGG TTTACCTGCT CTCGTTGTAG CAATTTCTGT TGGATTCACC
    AAGGCAAAGG GATATGGCAC TGTTAACTAT TGCTGGCTGT CTCTGGAAGG AGGGCTGTTG
    TACGCATTTG TGGGACCAGC TGCTGCTGTC GTCCTGGTTA ACATGGTTAT CGGGATTCTG
    GTTTTTAACA AACTGGTGTC CAAAGATGGC ATTACTGATA TGAAACTCAA GGAGAGGGCC
    GGGGCGTCGC TGTGGAGCTC CTGCGTTGTT CTGCCGTTGC TGGCGCTCAC TTGGATGTCG
    GCAGTCTTGG CTATTACTGA CCGCCGTTCA GCCTTGTTTC AAATCCTTTT TGCCGTCTTT
    GACTCCCTCG AGGGCTTTGT TATCGTGATG GTGCATTGCA TCCTACGCAG AGAGGTTCAA
    GAAGCAGTAA AGTGTCGTGT TGTTGACCGT CAAGAAGATG GAAACGGTGA CTCTGGTGGA
    TCCTTCCAGA ACGGTCATGC TCAGCTGATG ACTGACTTTG AAAAGGATGT GGACATAGCT
    TGTCGATCAG GCACCCTGAA GCGTCCCTCC CAGCAAGGCG AGGAGAAGGT TCCATCCCAG
    CAGATCACAG TACAGAAAGG CTCCAACTTC AACACCCTTC CTGCCAGCAT GGCAAAGGTC
    CACCTGCAAA ACGTAGCCAA CTACGCCAGT CACACTCTCA CCATGCGCCG CGACAAAGGC
    GCCATCGCCG GGGTCAGCAC GGAGTTACCC GGGGCCAAAT CTATTTACAT CTGTGACGGC
    GAGCTCTTCA AACAGCTTGA CGGAGCACCG GGAGACATTC CTGAAGGAGG TGGTGGAGGT
    GCAGGTCAGG GTTATGTGCT TTTGCCCAAC AACAACAACA CCCTTAGGCC TACGAAGGGT
    GGCAAGGAAG ACCCGGCCGC CAAATATAAC ATCAGTATCG AACAGTTACC CCAGACAAGG
    CTGATCCACC TGGCCAATCC TGTTGGCACC GATCCCGTGG CAGGCTTCGG GCTGAAGAGC
    TTGCCCACCG ACCGAGTCAG CGTTTCATGC TCGGAGAGGG AGTCCCCGGT GCAGACGGTG
    CAGAATATCT CCAGCGAGTC CCAGATGACA AATACGTGTG AGCAAGGGGA TTCTGGGAAC
    TCTGGGATGT CTAAGAGCGA GACTGTCTCC ACACTGTCCA TGAGCTCTCT GGAGAGACGA
    AAATCACGCT ATGCAGAGCT GGACTTTGAG AAGATCATGC ACACACGGAA GCGTCATCAG
    GACATGTTTC AGGACCTGAA TCGCAAACTG CAGCACGCAG AGAAGGACAG AGACTCTCCT
    CCAGTCGACT GCAAGCCTGG AAAGAGATGG AGCATTTCGT CAGGAGGAAG CGACAAAACC
    AACCTTAGTG ACAAGCAGCA GACCCCCAGT AAGCGGGCAT GGGAGGGCAT CAGGAAGACT
    CACTCTCCTC CATCATGGGT GCGCAAGGAG CTGGAGCCGC TGCAGGCCTC ACCGCTGGAG
    ATGCAGGCAG TGGAGTGGGA GAAGGCCAGT GCCACCATCC CTCTAGTGGG CCAGGAGATC
    ATGGACCTGC AGACGGAGGT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44104
    Clone ID Related Accession (Same CDS sequence) XM_009292562.2 , XM_009292562.3
    Accession Version XM_009292562.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4644bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product brain-specific angiogenesis inhibitor 1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009292562.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCA AAGGCCGGCT TGGCTCTCGT
    AGCCAATCCC TGCGCTCGGT GGACAGCCGT AAACGAGAGG ACTCTGATAA GACCCGCAGC
    GGACAGCAGT CTTCCCAAAC AGTGGATTCT CCGTCGGGTG AGGAGTGGTC TGCGTGGAGC
    GTGTGTTCGG CCACGTGTGG TGAGGGCTGG CAGAGCCGCA CACGCTTCTG CGTCTCCGTG
    TCGTACAGCA CGCAGTGCAG CGGGCCACTG CGCGAACAGA GACCCTGCAA TAACACTGCT
    GTGTGTCCAG AACATGGGGC GTGGGGCGAG TGGACACCAT GGAGCTTGTG TTCATCAACA
    TGCGGCCGTG GCTACAGGGA ACGCAACCGC TCCTGCAAGC CACCCCAGTT CGGAGGAAAC
    CCCTGTGATG GTCCAGAGAA ACAGACTAAA TTCTGCAACA TCGCTGTATG CCCAGTGGAT
    GGCGTGTGGA ACGAGTGGTC TGGTTGGAGT TCCTGTTCTT CAACTTGTTC GAATGGAACA
    ATGCAGCGAA TTCGGGAGTG TAACGGGCCC TCGTATGGGG GCTTAGAGTG TCAAGGCGAG
    TGGCACGAGA CACGGGATTG CTTCCTTAGA GAATGTCCTG TGGATGGCCA ATGGCAACTT
    TGGAGCACTT GGTCTGGTTG CACCAAAACC TGTGGTGGAG GAAGTCAGCA GAGACAGAGG
    CTGTGTTATG GTCCCTTTTT TGGGGGAGAG GCATGCCCGG GGGACCGAGA AGAAGTTAGA
    CGTTGCAATG AGAAGCGATG CCCAGAGCCA CATGAAATCT GTGATGAGGA GAATTTCTCT
    AATGTAGTGT GGAAGAAAAC ACCTGCAGGG GACACAGCGG CTGTCCGCTG CCCACCAAAT
    GCTGTAGGAT TAATTCTACG GCGATGTTCA TTAGACAATG AGGGTATAGC ATACTGGGAG
    AACCCAACAT ATATGAAGTG CGTCTCAAAT GATTACAGAA GTATCCAAAC ACTGACCAGA
    GATCACCTGT CCAAGGCGCA GCGAGGGCTT GTGGGGGATG GTGTTTCTGA AGTGATGACA
    AAGCTGAGGG TGACGTCAAG TGATGGAACG AGCTACAGCG GAGATCTTTT GGCTATCATA
    GATGTGCTAA AAAACATGAC AGAGATATTT AGGAGATCCT ACTACAGCCC CAGTAATGCA
    GACATGAGGA ATTTTGTGCA GTCTGTGAGT AACTTACTGA TGGAGGAAAA CAGGGACAGG
    TGGGAGGAAG CACAACTGCT GGGACCCAAT GTTAAGGAGC TTTTTCGTCT AGTAGAGGAC
    TTTGTGGACG TCATCAGCCT TCGCATGAAG GACTTCCAGG ATACTTATGA GGTCACGGAT
    AATCTAGTGT TGAGCATTCA CAAGCGGCCC GTGGCTGGAA ATGCAGATAT CACTTTCCCT
    ATGAAGGGCT GGAGGGGGAT GGTGGACTGG GCTCGCAGCT CTGAGGACAA AGTCAGAGTG
    GCCAAAAACA TCCTGCTCAC TGGAAAACCG GAAGAAGATG ACGCTTCAGC TTTTGTGACT
    GGGATCGTCC TGTACAGAAA CTTAGGCAGT ATTCTCTCTC TGCAGAGAAA TAGCACAGTG
    CTGAACTCCA AGGTGATCTC TGTGACAATC AAGCCCACTC CCAGCTCTCT CTCGAGTCCA
    CTAGAGATTG AGTTCTCACA CCTTTACAAT GGAACCACAA ACCAAACCTG CATTTCCTGG
    GATGAGAACG ACAGCTCGTC GTTGCTGGGC TCCTGGTCTG CGCGAGGCTG TAGAGCCGTG
    CCCGTCGACT CCAGCACAAC CAAATGCTTG TGTGACAGAC TCTCCACCTT CGCCATTTTA
    GCACGCCTCA ATCCCGATGT GAACATGGAT AAGACCCAGC TTCCATCTGT GACTCTGATC
    GTGGGCTGCG GGGTCTCGTC CCTCACCCTG CTCCTCCTCA TCATCATCTA TGTTTCTGTT
    TGGAGGTACA TCCGCTCAGA GCGCTCTGTC ATCCTCATCA ACTTCTGTCT GTCCATCATC
    TCCTCCAATG CGCTTATACT CATCGGTCAA ACTCAGACAC GGAACAAGGT TGTTTGTACT
    CTCGTGGCTG CGTTTCTGCA CTTTTTCTTC CTGTCCTCTT TCTGTTGGGT TCTAACGGAG
    GCATGGCAGT CGTACATGGC TGTGACCGGC CGCCTTAGAA ACCGTATCAT ACGCAAGCGT
    TTCCTTTGCC TTGGTTGGGG TTTACCTGCT CTCGTTGTAG CAATTTCTGT TGGATTCACC
    AAGGCAAAGG GATATGGCAC TGTTAACTAT TGCTGGCTGT CTCTGGAAGG AGGGCTGTTG
    TACGCATTTG TGGGACCAGC TGCTGCTGTC GTCCTGGTTA ACATGGTTAT CGGGATTCTG
    GTTTTTAACA AACTGGTGTC CAAAGATGGC ATTACTGATA TGAAACTCAA GGAGAGGGCC
    GGGGCGTCGC TGTGGAGCTC CTGCGTTGTT CTGCCGTTGC TGGCGCTCAC TTGGATGTCG
    GCAGTCTTGG CTATTACTGA CCGCCGTTCA GCCTTGTTTC AAATCCTTTT TGCCGTCTTT
    GACTCCCTCG AGGGCTTTGT TATCGTGATG GTGCATTGCA TCCTACGCAG AGAGGTTCAA
    GAAGCAGTAA AGTGTCGTGT TGTTGACCGT CAAGAAGATG GAAACGGTGA CTCTGGTGGA
    TCCTTCCAGA ACGGTCATGC TCAGCTGATG ACTGACTTTG AAAAGGATGT GGACATAGCT
    TGTCGATCAG GCACCCTGAA GCGTCCCTCC CAGCAAGGCG AGGAGAAGGT TCCATCCCAG
    CAGATCACAG TACAGAAAGG CTCCAACTTC AACACCCTTC CTGCCAGCAT GGCAAAGGTC
    CACCTGCAAA ACGTAGCCAA CTACGCCAGT CACACTCTCA CCATGCGCCG CGACAAAGGC
    GCCATCGCCG GGGTCAGCAC GGAGTTACCC GGGGCCAAAT CTATTTACAT CTGTGACGGC
    GAGCTCTTCA AACAGCTTGA CGGAGCACCG GGAGACATTC CTGAAGGAGG TGGTGGAGGT
    GCAGGTCAGG GTTATGTGCT TTTGCCCAAC AACAACAACA CCCTTAGGCC TACGAAGGGT
    GGCAAGGAAG ACCCGGCCGC CAAATATAAC ATCAGTATCG AACAGTTACC CCAGACAAGG
    CTGATCCACC TGGCCAATCC TGTTGGCACC GATCCCGTGG CAGGCTTCGG GCTGAAGAGC
    TTGCCCACCG ACCGAGTCAG CGTTTCATGC TCGGAGAGGG AGTCCCCGGT GCAGACGGTG
    CAGAATATCT CCAGCGAGTC CCAGATGACA AATACGTGTG AGCAAGGGGA TTCTGGGAAC
    TCTGGGATGT CTAAGAGCGA GACTGTCTCC ACACTGTCCA TGAGCTCTCT GGAGAGACGA
    AAATCACGCT ATGCAGAGCT GGACTTTGAG AAGATCATGC ACACACGGAA GCGTCATCAG
    GACATGTTTC AGGACCTGAA TCGCAAACTG CAGCACGCAG AGAAGGACAG AGACTCTCCT
    CCAGTCGACT GCAAGCCTGG AAAGAGATGG AGCATTTCGT CAGGAGGAAG CGACAAAACC
    AACCTTAGTG ACAAGCAGCA GACCCCCAGT AAGCGGGCAT GGGAGGGCAT CAGGAAGACT
    CACTCTCCTC CATCATGGGT GCGCAAGGAG CTGGAGCCGC TGCAGGCCTC ACCGCTGGAG
    ATGCAGGCAG TGGAGTGGGA GAAGGCCAGT GCCACCATCC CTCTAGTGGG CCAGGAGATC
    ATGGACCTGC AGACGGAGGT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290837.1
    CDS747..5390
    Translation

    Target ORF information:

    RefSeq Version XM_009292562.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio adhesion G protein-coupled receptor B1a (adgrb1a), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292562.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGATATCAA AATTTTTAGG TAACTGTTCA TCGCTGATCC TGCTGTTCCC TCTTCTTCAA 
    CTGCTGCGGA TGGACATGCT CAACGCTGCC CCCTCTGGTC CGGAGACTGA CAGCTGCTCC
    ACATTAGTCC AGAGCCGCTT CTTTGGATTC TTCCTCTCGT CTTCTGCATT TCCCAGCATG
    CCCTGCACCT GGACCCTACA GAACCCTGAT CCACGGCGCT ACACCATCTT CATCAAAGTC
    ACAAAACCAA CTAGAGACTG CATTGCCCGG CAGCACCGGA CCTTCCAGTT TGACTCCTTC
    TTAGAGACGA CTCGGACCTT TCTCGGAATG GAGAGCTTTG ATGAAGTGGT GAAGCTGTGC
    GATGCCTCTA CCCATGTTGC CTTCTTGGAA GCTGGGAAAC AATTCCTGCA GATCCGGAAA
    GGGCTGCCTA GAGCAGGCGC TGGGTCTAGA AATGGGGATG GGGAATTTAA AGTGGAGTAT
    CTTGTGGTTG GGAAGCGTAA CCCCAGCATG GCTGCGTGTC AAATGCTTTG CCAGTGGTTG
    GAGGACTGCC TGACCTACAG TTCTTCCACC CGACCCTGTG GCATCATGCA GACCCCATGC
    CAATGCTGGG ACCAGCCTCC ACTAGACAAA GAATCCAATG GATGCTATCG AGATGGGGTC
    TTTCTGGAAA ACTGCATCCC TGTTGTGAAA GAAGGAGCGT CTGAAACTGA CAATAGCGGT
    GGCTGGAGTG GGTGGGGCAA CTGGGCGGAG TGTAGCAGTG AATGCGGGGG TGGAGTCCAG
    AAGCGAACGC GCAACTGTCA ATCACCTCCT GATGAAGCCT ACCTGTGCGA AGGTGTTCTG
    GAAGAAGGTC GTCCATGCAA TGCTCAGCCC TGCATCGGCA AAGGCCGGCT TGGCTCTCGT
    AGCCAATCCC TGCGCTCGGT GGACAGCCGT AAACGAGAGG ACTCTGATAA GACCCGCAGC
    GGACAGCAGT CTTCCCAAAC AGTGGATTCT CCGTCGGGTG AGGAGTGGTC TGCGTGGAGC
    GTGTGTTCGG CCACGTGTGG TGAGGGCTGG CAGAGCCGCA CACGCTTCTG CGTCTCCGTG
    TCGTACAGCA CGCAGTGCAG CGGGCCACTG CGCGAACAGA GACCCTGCAA TAACACTGCT
    GTGTGTCCAG AACATGGGGC GTGGGGCGAG TGGACACCAT GGAGCTTGTG TTCATCAACA
    TGCGGCCGTG GCTACAGGGA ACGCAACCGC TCCTGCAAGC CACCCCAGTT CGGAGGAAAC
    CCCTGTGATG GTCCAGAGAA ACAGACTAAA TTCTGCAACA TCGCTGTATG CCCAGTGGAT
    GGCGTGTGGA ACGAGTGGTC TGGTTGGAGT TCCTGTTCTT CAACTTGTTC GAATGGAACA
    ATGCAGCGAA TTCGGGAGTG TAACGGGCCC TCGTATGGGG GCTTAGAGTG TCAAGGCGAG
    TGGCACGAGA CACGGGATTG CTTCCTTAGA GAATGTCCTG TGGATGGCCA ATGGCAACTT
    TGGAGCACTT GGTCTGGTTG CACCAAAACC TGTGGTGGAG GAAGTCAGCA GAGACAGAGG
    CTGTGTTATG GTCCCTTTTT TGGGGGAGAG GCATGCCCGG GGGACCGAGA AGAAGTTAGA
    CGTTGCAATG AGAAGCGATG CCCAGAGCCA CATGAAATCT GTGATGAGGA GAATTTCTCT
    AATGTAGTGT GGAAGAAAAC ACCTGCAGGG GACACAGCGG CTGTCCGCTG CCCACCAAAT
    GCTGTAGGAT TAATTCTACG GCGATGTTCA TTAGACAATG AGGGTATAGC ATACTGGGAG
    AACCCAACAT ATATGAAGTG CGTCTCAAAT GATTACAGAA GTATCCAAAC ACTGACCAGA
    GATCACCTGT CCAAGGCGCA GCGAGGGCTT GTGGGGGATG GTGTTTCTGA AGTGATGACA
    AAGCTGAGGG TGACGTCAAG TGATGGAACG AGCTACAGCG GAGATCTTTT GGCTATCATA
    GATGTGCTAA AAAACATGAC AGAGATATTT AGGAGATCCT ACTACAGCCC CAGTAATGCA
    GACATGAGGA ATTTTGTGCA GTCTGTGAGT AACTTACTGA TGGAGGAAAA CAGGGACAGG
    TGGGAGGAAG CACAACTGCT GGGACCCAAT GTTAAGGAGC TTTTTCGTCT AGTAGAGGAC
    TTTGTGGACG TCATCAGCCT TCGCATGAAG GACTTCCAGG ATACTTATGA GGTCACGGAT
    AATCTAGTGT TGAGCATTCA CAAGCGGCCC GTGGCTGGAA ATGCAGATAT CACTTTCCCT
    ATGAAGGGCT GGAGGGGGAT GGTGGACTGG GCTCGCAGCT CTGAGGACAA AGTCAGAGTG
    GCCAAAAACA TCCTGCTCAC TGGAAAACCG GAAGAAGATG ACGCTTCAGC TTTTGTGACT
    GGGATCGTCC TGTACAGAAA CTTAGGCAGT ATTCTCTCTC TGCAGAGAAA TAGCACAGTG
    CTGAACTCCA AGGTGATCTC TGTGACAATC AAGCCCACTC CCAGCTCTCT CTCGAGTCCA
    CTAGAGATTG AGTTCTCACA CCTTTACAAT GGAACCACAA ACCAAACCTG CATTTCCTGG
    GATGAGAACG ACAGCTCGTC GTTGCTGGGC TCCTGGTCTG CGCGAGGCTG TAGAGCCGTG
    CCCGTCGACT CCAGCACAAC CAAATGCTTG TGTGACAGAC TCTCCACCTT CGCCATTTTA
    GCACGCCTCA ATCCCGATGT GAACATGGAT AAGACCCAGC TTCCATCTGT GACTCTGATC
    GTGGGCTGCG GGGTCTCGTC CCTCACCCTG CTCCTCCTCA TCATCATCTA TGTTTCTGTT
    TGGAGGTACA TCCGCTCAGA GCGCTCTGTC ATCCTCATCA ACTTCTGTCT GTCCATCATC
    TCCTCCAATG CGCTTATACT CATCGGTCAA ACTCAGACAC GGAACAAGGT TGTTTGTACT
    CTCGTGGCTG CGTTTCTGCA CTTTTTCTTC CTGTCCTCTT TCTGTTGGGT TCTAACGGAG
    GCATGGCAGT CGTACATGGC TGTGACCGGC CGCCTTAGAA ACCGTATCAT ACGCAAGCGT
    TTCCTTTGCC TTGGTTGGGG TTTACCTGCT CTCGTTGTAG CAATTTCTGT TGGATTCACC
    AAGGCAAAGG GATATGGCAC TGTTAACTAT TGCTGGCTGT CTCTGGAAGG AGGGCTGTTG
    TACGCATTTG TGGGACCAGC TGCTGCTGTC GTCCTGGTTA ACATGGTTAT CGGGATTCTG
    GTTTTTAACA AACTGGTGTC CAAAGATGGC ATTACTGATA TGAAACTCAA GGAGAGGGCC
    GGGGCGTCGC TGTGGAGCTC CTGCGTTGTT CTGCCGTTGC TGGCGCTCAC TTGGATGTCG
    GCAGTCTTGG CTATTACTGA CCGCCGTTCA GCCTTGTTTC AAATCCTTTT TGCCGTCTTT
    GACTCCCTCG AGGGCTTTGT TATCGTGATG GTGCATTGCA TCCTACGCAG AGAGGTTCAA
    GAAGCAGTAA AGTGTCGTGT TGTTGACCGT CAAGAAGATG GAAACGGTGA CTCTGGTGGA
    TCCTTCCAGA ACGGTCATGC TCAGCTGATG ACTGACTTTG AAAAGGATGT GGACATAGCT
    TGTCGATCAG GCACCCTGAA GCGTCCCTCC CAGCAAGGCG AGGAGAAGGT TCCATCCCAG
    CAGATCACAG TACAGAAAGG CTCCAACTTC AACACCCTTC CTGCCAGCAT GGCAAAGGTC
    CACCTGCAAA ACGTAGCCAA CTACGCCAGT CACACTCTCA CCATGCGCCG CGACAAAGGC
    GCCATCGCCG GGGTCAGCAC GGAGTTACCC GGGGCCAAAT CTATTTACAT CTGTGACGGC
    GAGCTCTTCA AACAGCTTGA CGGAGCACCG GGAGACATTC CTGAAGGAGG TGGTGGAGGT
    GCAGGTCAGG GTTATGTGCT TTTGCCCAAC AACAACAACA CCCTTAGGCC TACGAAGGGT
    GGCAAGGAAG ACCCGGCCGC CAAATATAAC ATCAGTATCG AACAGTTACC CCAGACAAGG
    CTGATCCACC TGGCCAATCC TGTTGGCACC GATCCCGTGG CAGGCTTCGG GCTGAAGAGC
    TTGCCCACCG ACCGAGTCAG CGTTTCATGC TCGGAGAGGG AGTCCCCGGT GCAGACGGTG
    CAGAATATCT CCAGCGAGTC CCAGATGACA AATACGTGTG AGCAAGGGGA TTCTGGGAAC
    TCTGGGATGT CTAAGAGCGA GACTGTCTCC ACACTGTCCA TGAGCTCTCT GGAGAGACGA
    AAATCACGCT ATGCAGAGCT GGACTTTGAG AAGATCATGC ACACACGGAA GCGTCATCAG
    GACATGTTTC AGGACCTGAA TCGCAAACTG CAGCACGCAG AGAAGGACAG AGACTCTCCT
    CCAGTCGACT GCAAGCCTGG AAAGAGATGG AGCATTTCGT CAGGAGGAAG CGACAAAACC
    AACCTTAGTG ACAAGCAGCA GACCCCCAGT AAGCGGGCAT GGGAGGGCAT CAGGAAGACT
    CACTCTCCTC CATCATGGGT GCGCAAGGAG CTGGAGCCGC TGCAGGCCTC ACCGCTGGAG
    ATGCAGGCAG TGGAGTGGGA GAAGGCCAGT GCCACCATCC CTCTAGTGGG CCAGGAGATC
    ATGGACCTGC AGACGGAGGT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.