RHBDF1B cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	RHBDF1B
Entrez Gene ID	563403
Full Name	rhomboid 5 homolog 1b (Drosophila)
Synonyms	si:ch211-218l14.9
General protein information	Preferred Names rhomboid 5 homolog 1b (Drosophila) Names inactive rhomboid protein 1-like zmp:0000000557
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021472527.1	XP_021328202.1	inactive rhomboid protein 1-like isoform X2
XM_021480300.1	XP_021335975.1	inactive rhomboid protein 1-like isoform X1
XM_009306705.3	XP_009304980.1	inactive rhomboid protein 1-like isoform X2
XM_021472526.1	XP_021328201.1	inactive rhomboid protein 1-like isoform X1
XM_021472528.1	XP_021328203.1	inactive rhomboid protein 1-like isoform X3
XM_009306705.2	XP_009304980.1	inactive rhomboid protein 1-like

Pathways from BioSystems
Gene Ontology

The following RHBDF1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RHBDF1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa145360	XM_021472528.1 Latest version!	Danio rerio rhomboid 5 homolog 1b (Drosophila) (rhbdf1b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
ODa44078	XM_009306705.3 Latest version!	Danio rerio rhomboid 5 homolog 1b (Drosophila) (rhbdf1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa145358	XM_021472526.1 Latest version!	Danio rerio rhomboid 5 homolog 1b (Drosophila) (rhbdf1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ODa145359	XM_021472527.1 Latest version!	Danio rerio rhomboid 5 homolog 1b (Drosophila) (rhbdf1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ODa148338	XM_021480300.1 Latest version!	Danio rerio rhomboid 5 homolog 1b (Drosophila) (rhbdf1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
ODa44078	XM_009306705.2	Danio rerio rhomboid 5 homolog 1b (Drosophila) (rhbdf1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RHBDF1B ( XM_021472528.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021328203.1 Danio rerio rhomboid 5 homolog 1b (Drosophila) (rhbdf1b), transcript variant X3, mRNA.

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CloneID	ODa145360
Clone ID Related Accession (Same CDS sequence)	XM_021472528.1
Accession Version	XM_021472528.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	inactive rhomboid protein 1-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394837.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGATG CTGAGGATGA GAGCAGGAAT GACAGCCTGC AGAGAAAGAA GCCGCCCTGG 
CTCAGACTGG ATATCCCCAC TGTGGAGGAA CACAATCACT TCCTTCAGCC TGTTATGCGT 
AACAGTTTGT ACCTGCGGAG TGCCAGTGTG CCCACTGAAA ATATTCAGCT CAGATGCCCG 
CCAGTTGACA TCAACGGCTG TCGCAGACCC TCAGTCCTGC GTAAACCCTC CATCTCACAG 
ACCATCAAGA GTCCCACAGG GAAGAGAGTG CGCTTTGAGC AGATGAGCAC CGTCCTGGTT 
AAAGGCCACT GTGTCTCACG TCTGGAAGCC GACAGACGTC AGTCACTGCC CCAAATCGTC 
CTCAGGGGCA CAGCAGACTG GTTTGGGGTC AGCAATGATT TGGATGCCAC CCAGAAATGG 
CAGCGGAAGA GTTTGCGTCA CTGCACCCAG CGCTACGGCA AACTAAAGCC TCAGGTGATC 
CGAGAGATGG ACCTGCCCAG CCAAGACAAT CTCTCCATGA CCAGCACCGA AACCCCTCCT 
CCTCTATATG TCCCACCGCC ACATCATGGC ATGCAGAGGA TCGTAGACCC GCTGGCTCGA 
GGTCGAGCGT TCCGTAATGT GGATGAGGTG GATGGTGTGA ATGTACCTCA GACCCCCATC 
ACCCCCTGCG CCGCTTCCCT GTGCTCCTTC ACCAGCTCCC GCTCCGGCTT TAACCGGCCT 
CCCCGCAGAC GCAAGCGTGA GTCTGTCGCC AAGATGGGCT TCAGAGCTGC TGCTGCTCTG 
GTCAAGGGTC GTTCTCTGCG GGACGGGGCT GTTCGCCGAT TGCCGAGGCG CAGTTTTGCC 
GCTGCTAGTT TCCTGGATGA GGACACAGTG GATTTCCCTG ATGAACTGGA CACCTCTTTC 
TTCGCCAAGG ATGGGGTTAC CCATGAAGAG CTGTCCACAT ACACAGACGA GGTGTTTGAA 
TCACCTTCTG AAGCTGCCAT TAAAGAAACA GATGCCAGTC GAGCGGGAGA TGAGCGGGAC 
CTGACGGGGA GTGCCCTGGA GAAGACCGAG CTGGAGCGGA GCCATCTGAT GCTGCCTCTG 
GAGAGGGGTT GGCGTAAAGC AAAGGATGGT GCTCTGGTTC AGCCTAAAGT GCGCTTACGG 
CAAGAGGTGG TGAGTGTGAC TGGGCAGCGG CGTGGTCAGC GCATTACTGT GCCTGTTAAA 
AAACTCTTTG CTAAGGAAAA ACGTCCTTAT GGGCTTGGCA TGGTGGGGAA ACTGACCAAC 
CGCACCTATC GCAAACGCAT TGACAGCTAT GTGAAGAAAC AGATTGAAGA CATGAACGAC 
CACAGGTATT TACCACAGTG TTGCTGGAGC TCTTGCAGAC GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328203.1
CDS	283..1647
Translation	MADAEDESRNDSLQRKKPPWLRLDIPTVEEHNHFLQPVMRNSLYLRSASVPTENIQLRCPPVDINGCRRPSVLRKPSISQTIKSPTGKRVRFEQMSTVLVKGHCVSRLEADRRQSLPQIVLRGTADWFGVSNDLDATQKWQRKSLRHCTQRYGKLKPQVIREMDLPSQDNLSMTSTETPPPLYVPPPHHGMQRIVDPLARGRAFRNVDEVDGVNVPQTPITPCAASLCSFTSSRSGFNRPPRRRKRESVAKMGFRAAAALVKGRSLRDGAVRRLPRRSFAAASFLDEDTVDFPDELDTSFFAKDGVTHEELSTYTDEVFESPSEAAIKETDASRAGDERDLTGSALEKTELERSHLMLPLERGWRKAKDGALVQPKVRLRQEVVSVTGQRRGQRITVPVKKLFAKEKRPYGLGMVGKLTNRTYRKRIDSYVKKQIEDMNDHRYLPQCCWSSCRR

Target ORF information:

RefSeq Version	XM_021472528.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio rhomboid 5 homolog 1b (Drosophila) (rhbdf1b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472528.1

ORF Insert Sequence:

ATGGCCGATG CTGAGGATGA GAGCAGGAAT GACAGCCTGC AGAGAAAGAA GCCGCCCTGG 
CTCAGACTGG ATATCCCCAC TGTGGAGGAA CACAATCACT TCCTTCAGCC TGTTATGCGT 
AACAGTTTGT ACCTGCGGAG TGCCAGTGTG CCCACTGAAA ATATTCAGCT CAGATGCCCG 
CCAGTTGACA TCAACGGCTG TCGCAGACCC TCAGTCCTGC GTAAACCCTC CATCTCACAG 
ACCATCAAGA GTCCCACAGG GAAGAGAGTG CGCTTTGAGC AGATGAGCAC CGTCCTGGTT 
AAAGGCCACT GTGTCTCACG TCTGGAAGCC GACAGACGTC AGTCACTGCC CCAAATCGTC 
CTCAGGGGCA CAGCAGACTG GTTTGGGGTC AGCAATGATT TGGATGCCAC CCAGAAATGG 
CAGCGGAAGA GTTTGCGTCA CTGCACCCAG CGCTACGGCA AACTAAAGCC TCAGGTGATC 
CGAGAGATGG ACCTGCCCAG CCAAGACAAT CTCTCCATGA CCAGCACCGA AACCCCTCCT 
CCTCTATATG TCCCACCGCC ACATCATGGC ATGCAGAGGA TCGTAGACCC GCTGGCTCGA 
GGTCGAGCGT TCCGTAATGT GGATGAGGTG GATGGTGTGA ATGTACCTCA GACCCCCATC 
ACCCCCTGCG CCGCTTCCCT GTGCTCCTTC ACCAGCTCCC GCTCCGGCTT TAACCGGCCT 
CCCCGCAGAC GCAAGCGTGA GTCTGTCGCC AAGATGGGCT TCAGAGCTGC TGCTGCTCTG 
GTCAAGGGTC GTTCTCTGCG GGACGGGGCT GTTCGCCGAT TGCCGAGGCG CAGTTTTGCC 
GCTGCTAGTT TCCTGGATGA GGACACAGTG GATTTCCCTG ATGAACTGGA CACCTCTTTC 
TTCGCCAAGG ATGGGGTTAC CCATGAAGAG CTGTCCACAT ACACAGACGA GGTGTTTGAA 
TCACCTTCTG AAGCTGCCAT TAAAGAAACA GATGCCAGTC GAGCGGGAGA TGAGCGGGAC 
CTGACGGGGA GTGCCCTGGA GAAGACCGAG CTGGAGCGGA GCCATCTGAT GCTGCCTCTG 
GAGAGGGGTT GGCGTAAAGC AAAGGATGGT GCTCTGGTTC AGCCTAAAGT GCGCTTACGG 
CAAGAGGTGG TGAGTGTGAC TGGGCAGCGG CGTGGTCAGC GCATTACTGT GCCTGTTAAA 
AAACTCTTTG CTAAGGAAAA ACGTCCTTAT GGGCTTGGCA TGGTGGGGAA ACTGACCAAC 
CGCACCTATC GCAAACGCAT TGACAGCTAT GTGAAGAAAC AGATTGAAGA CATGAACGAC 
CACAGGTATT TACCACAGTG TTGCTGGAGC TCTTGCAGAC GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHBDF1B ( XM_009306705.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009304980.1 Danio rerio rhomboid 5 homolog 1b (Drosophila) (rhbdf1b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa44078
Clone ID Related Accession (Same CDS sequence)	XM_009306705.3 , XM_009306705.2
Accession Version	XM_009306705.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	inactive rhomboid protein 1-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306705.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGATG CTGAGGATGA GAGCAGGAAT GACAGCCTGC AGAGAAAGAA GCCGCCCTGG 
CTCAGACTGG ACATCCCCAC TGTGGAGGAA CACAATCACT TCCTTCAGCC TGTCATGCGT 
AACAGTTTGT ACCTGCGGAG TGCCAGTGTG CCCACTGAAA ATATTCAGCT CAGATGCCCG 
CCAGTTGACA TCAACGGCTG TCGCAGACCC TCAGTCCTGC GCAAACCCTC CATCTCACAG 
ACCATCAAGA GGGGCACAGC AGACTGGTTT GGGGTCAGCA ATGATTTGGA TGCCACCCAG 
AAATGGCAGC GGAAGAGTTT GCGTCACTGC ACCCAGCGCT ACGGCAAACT AAAGCCTCAG 
GTGATCCGAG AGATGGACCT GCCCAGCCAA GACAATCTCT CCATGACCAG CACCGAAACC 
CCTCCTCCTC TATATGTCCC ACCGCCACAT CATGGCATGC AGAGGATCGT AGACCCGCTG 
GCTCGAGGTC GAGCGTTCCG TAATGTGGAT GAGGTGGATG GTGTGAATGT ACCTCAGACC 
CCCATCACCC CCTGCGCCGC TTCCCTGTGC TCCTTCACCA GCTCCCGCTC CGGCTTCAAC 
CGGCCTCCCC GCAGACGCAA GCGTGAGTCT GTCGCCAAGA TGGGCTTCAG AGCTGCTGCT 
GCTCTGGTCA AGGGTCGTTC TCTGCGGGAC GGGGCTGTTC GCCGATTGCC GAGGCGCAGT 
TTTGCCGCTG CTAGTTTCCT GGATGAGGAC ACAGTGGATT TCCCTGATGA ACTGGACACC 
TCTTTCTTCG CCAAGGATGG GGTTACCCAT GAAGAGCTGT CCACATACGC AGACGAGGTG 
TTTGAATCAC CTTCTGAAGC TGCCATTAAA GAAACAGATG CCAGTCGAGC GGGAGATGAG 
CGGGACCTGA CGGGGAGTGC CCTGGAGAAG ACCGAGCTGG AGCGGAGCCA TCTGATGCTG 
CCTCTGGAGA GGGGTTGGCG TAAAGCAAAG GATGGTGCTC TGGTTCAGCC TAAAGTGCGC 
TTACGGCAAG AGGTGGTGAG TGTGACTGGG CAGCGGCGTG GTCAGCGCAT TACTGTGCCT 
GTTAAAAAAC TCTTTGCTAA GGAAAAACGT CCTTATGGGC TTGGCATGGT GGGGAAACTG 
ACCAACCGCA CCTATCGCAA ACGCATTGAC AGCTATGTGA AGAAACAGAT CGAAGACATG 
AACGACCACA GGCCTTTCTT CACATATTGG ATCACCTTTG TTCATTTGTT GATTACCGTC 
TTGGCGGTTT CCATCTATGG CATTGCACCA GTGGGCTTTT CACAACATGA GACTGTTGAT 
TCTGTCTTAC GAAACAAAGG AGTTTATGAG AATGTCAGAT TCATGCAGCA AGAGAACTTC 
TGGGTTGGGC CAAGCTCGGA GGCGCTGATT CACTTGGGGG CGAAGTTTTC CCCCTGTATG 
CGACAGGATG AAGAGGTTCA CCAGCTCATC CAGCAGAAAA AGGCCAGCGA AAAACACTCG 
GCCTGCTGCG TCCGCAATGA CCAGTCCGGC TGCGTTCAGA CCTCTCAGGA GGAGTGTTCA 
AGCACTCTGG CATTATGGGT AAAGTGGCCT CAGCACCCAA GTGCTCCAGT TCTAGGGGAT 
AAAGTGCGTC AGTACGGCTC CGTGTGCCAT CAAGACCCCA GGATTTGTCT GGAGCCTGCC 
TCTGTTTCTC CTCATGTGTG GCCTGATGAC ATCAGCAAGT GGCCGATATG CACCAGGTAC 
AGCACAGGGA ATCACACTAA CCTGCCTCAC ATCGACTGCG TCATCACTGG TCGACCCTGC 
TGCATCGGAA CGAAAGGAAG GTGTGAGATT ACATCGAGAG AGTACTGTGA CTTCATGAAG 
GGATACTTCC ATGAGGAGGC CACGCTCTGT TCACAGGTTC ATTGCATGGA TGATGTTTGT 
GGCCTCCTGC CTTTTCTGAA CCCAGAGATC CCAGACCAGT TCTATCGACT CTGGCTATCT 
CTGTTTCTTC ACGCCGGGAT CCTGCACTGC CTGGTGTCTG TGTGCTTCCA GATGACCATT 
CTAAGGGATT TGGAGAAGCT GGCAGGCTGG CTGAGAATCT CTATCATCTA TATCCTCAGC 
GGGATTACGG GCAACCTCGC CAGTGCCATC TTCTTACCGT ACAGAGCAGA GGTGGGTCCG 
GCTGGCTCTC AGTTTGGCAT CTTGGCCTGT CTGTTCGTGG AGCTGTTCCA GAGCTGGCAG 
ATCTTGGCAC GGCCGTGGAG GGCCTTCACC AAGCTCTCAT GTGTAGTGCT GTTCCTTTTC 
GCCTTTGGCC TGCTGCCCTG GATAGATAAT TTCGCTCACA TCTGTGGCTT CGTCTCGGGC 
TTCTTCCTGT CCTTCGCCTT CCTGCCCTAC ATCAGCTTCG GCCGCATGGA CATGTACCGC 
AAACGTCTAC AGATCCTCGT GGCACTTACC CTTTTTGTGG GCATCTTCTC CAGCTTCGTG 
GTACTCTTCT ATGTTTATCC AGTTAAATGC GAGTGGTGTG AGTTTCTCAC CTGCATCCCG 
CTGACGGACA AGTTTTGTGA GAAATACGAC CTCAATGCTC ACCTCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304980.1
CDS	286..2856
Translation	MADAEDESRNDSLQRKKPPWLRLDIPTVEEHNHFLQPVMRNSLYLRSASVPTENIQLRCPPVDINGCRRPSVLRKPSISQTIKRGTADWFGVSNDLDATQKWQRKSLRHCTQRYGKLKPQVIREMDLPSQDNLSMTSTETPPPLYVPPPHHGMQRIVDPLARGRAFRNVDEVDGVNVPQTPITPCAASLCSFTSSRSGFNRPPRRRKRESVAKMGFRAAAALVKGRSLRDGAVRRLPRRSFAAASFLDEDTVDFPDELDTSFFAKDGVTHEELSTYADEVFESPSEAAIKETDASRAGDERDLTGSALEKTELERSHLMLPLERGWRKAKDGALVQPKVRLRQEVVSVTGQRRGQRITVPVKKLFAKEKRPYGLGMVGKLTNRTYRKRIDSYVKKQIEDMNDHRPFFTYWITFVHLLITVLAVSIYGIAPVGFSQHETVDSVLRNKGVYENVRFMQQENFWVGPSSEALIHLGAKFSPCMRQDEEVHQLIQQKKASEKHSACCVRNDQSGCVQTSQEECSSTLALWVKWPQHPSAPVLGDKVRQYGSVCHQDPRICLEPASVSPHVWPDDISKWPICTRYSTGNHTNLPHIDCVITGRPCCIGTKGRCEITSREYCDFMKGYFHEEATLCSQVHCMDDVCGLLPFLNPEIPDQFYRLWLSLFLHAGILHCLVSVCFQMTILRDLEKLAGWLRISIIYILSGITGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQILARPWRAFTKLSCVVLFLFAFGLLPWIDNFAHICGFVSGFFLSFAFLPYISFGRMDMYRKRLQILVALTLFVGIFSSFVVLFYVYPVKCEWCEFLTCIPLTDKFCEKYDLNAHLH

Target ORF information:

RefSeq Version	XM_009306705.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio rhomboid 5 homolog 1b (Drosophila) (rhbdf1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306705.3

ORF Insert Sequence:

ATGGCCGATG CTGAGGATGA GAGCAGGAAT GACAGCCTGC AGAGAAAGAA GCCGCCCTGG 
CTCAGACTGG ACATCCCCAC TGTGGAGGAA CACAATCACT TCCTTCAGCC TGTCATGCGT 
AACAGTTTGT ACCTGCGGAG TGCCAGTGTG CCCACTGAAA ATATTCAGCT CAGATGCCCG 
CCAGTTGACA TCAACGGCTG TCGCAGACCC TCAGTCCTGC GCAAACCCTC CATCTCACAG 
ACCATCAAGA GGGGCACAGC AGACTGGTTT GGGGTCAGCA ATGATTTGGA TGCCACCCAG 
AAATGGCAGC GGAAGAGTTT GCGTCACTGC ACCCAGCGCT ACGGCAAACT AAAGCCTCAG 
GTGATCCGAG AGATGGACCT GCCCAGCCAA GACAATCTCT CCATGACCAG CACCGAAACC 
CCTCCTCCTC TATATGTCCC ACCGCCACAT CATGGCATGC AGAGGATCGT AGACCCGCTG 
GCTCGAGGTC GAGCGTTCCG TAATGTGGAT GAGGTGGATG GTGTGAATGT ACCTCAGACC 
CCCATCACCC CCTGCGCCGC TTCCCTGTGC TCCTTCACCA GCTCCCGCTC CGGCTTCAAC 
CGGCCTCCCC GCAGACGCAA GCGTGAGTCT GTCGCCAAGA TGGGCTTCAG AGCTGCTGCT 
GCTCTGGTCA AGGGTCGTTC TCTGCGGGAC GGGGCTGTTC GCCGATTGCC GAGGCGCAGT 
TTTGCCGCTG CTAGTTTCCT GGATGAGGAC ACAGTGGATT TCCCTGATGA ACTGGACACC 
TCTTTCTTCG CCAAGGATGG GGTTACCCAT GAAGAGCTGT CCACATACGC AGACGAGGTG 
TTTGAATCAC CTTCTGAAGC TGCCATTAAA GAAACAGATG CCAGTCGAGC GGGAGATGAG 
CGGGACCTGA CGGGGAGTGC CCTGGAGAAG ACCGAGCTGG AGCGGAGCCA TCTGATGCTG 
CCTCTGGAGA GGGGTTGGCG TAAAGCAAAG GATGGTGCTC TGGTTCAGCC TAAAGTGCGC 
TTACGGCAAG AGGTGGTGAG TGTGACTGGG CAGCGGCGTG GTCAGCGCAT TACTGTGCCT 
GTTAAAAAAC TCTTTGCTAA GGAAAAACGT CCTTATGGGC TTGGCATGGT GGGGAAACTG 
ACCAACCGCA CCTATCGCAA ACGCATTGAC AGCTATGTGA AGAAACAGAT CGAAGACATG 
AACGACCACA GGCCTTTCTT CACATATTGG ATCACCTTTG TTCATTTGTT GATTACCGTC 
TTGGCGGTTT CCATCTATGG CATTGCACCA GTGGGCTTTT CACAACATGA GACTGTTGAT 
TCTGTCTTAC GAAACAAAGG AGTTTATGAG AATGTCAGAT TCATGCAGCA AGAGAACTTC 
TGGGTTGGGC CAAGCTCGGA GGCGCTGATT CACTTGGGGG CGAAGTTTTC CCCCTGTATG 
CGACAGGATG AAGAGGTTCA CCAGCTCATC CAGCAGAAAA AGGCCAGCGA AAAACACTCG 
GCCTGCTGCG TCCGCAATGA CCAGTCCGGC TGCGTTCAGA CCTCTCAGGA GGAGTGTTCA 
AGCACTCTGG CATTATGGGT AAAGTGGCCT CAGCACCCAA GTGCTCCAGT TCTAGGGGAT 
AAAGTGCGTC AGTACGGCTC CGTGTGCCAT CAAGACCCCA GGATTTGTCT GGAGCCTGCC 
TCTGTTTCTC CTCATGTGTG GCCTGATGAC ATCAGCAAGT GGCCGATATG CACCAGGTAC 
AGCACAGGGA ATCACACTAA CCTGCCTCAC ATCGACTGCG TCATCACTGG TCGACCCTGC 
TGCATCGGAA CGAAAGGAAG GTGTGAGATT ACATCGAGAG AGTACTGTGA CTTCATGAAG 
GGATACTTCC ATGAGGAGGC CACGCTCTGT TCACAGGTTC ATTGCATGGA TGATGTTTGT 
GGCCTCCTGC CTTTTCTGAA CCCAGAGATC CCAGACCAGT TCTATCGACT CTGGCTATCT 
CTGTTTCTTC ACGCCGGGAT CCTGCACTGC CTGGTGTCTG TGTGCTTCCA GATGACCATT 
CTAAGGGATT TGGAGAAGCT GGCAGGCTGG CTGAGAATCT CTATCATCTA TATCCTCAGC 
GGGATTACGG GCAACCTCGC CAGTGCCATC TTCTTACCGT ACAGAGCAGA GGTGGGTCCG 
GCTGGCTCTC AGTTTGGCAT CTTGGCCTGT CTGTTCGTGG AGCTGTTCCA GAGCTGGCAG 
ATCTTGGCAC GGCCGTGGAG GGCCTTCACC AAGCTCTCAT GTGTAGTGCT GTTCCTTTTC 
GCCTTTGGCC TGCTGCCCTG GATAGATAAT TTCGCTCACA TCTGTGGCTT CGTCTCGGGC 
TTCTTCCTGT CCTTCGCCTT CCTGCCCTAC ATCAGCTTCG GCCGCATGGA CATGTACCGC 
AAACGTCTAC AGATCCTCGT GGCACTTACC CTTTTTGTGG GCATCTTCTC CAGCTTCGTG 
GTACTCTTCT ATGTTTATCC AGTTAAATGC GAGTGGTGTG AGTTTCTCAC CTGCATCCCG 
CTGACGGACA AGTTTTGTGA GAAATACGAC CTCAATGCTC ACCTCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHBDF1B ( XM_009306705.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009304980.1 Danio rerio rhomboid 5 homolog 1b (Drosophila) (rhbdf1b), mRNA.

Price & Availability↑

CloneID	ODa44078
Clone ID Related Accession (Same CDS sequence)	XM_009306705.3 , XM_009306705.2
Accession Version	XM_009306705.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	inactive rhomboid protein 1-like
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306705.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGATG CTGAGGATGA GAGCAGGAAT GACAGCCTGC AGAGAAAGAA GCCGCCCTGG 
CTCAGACTGG ACATCCCCAC TGTGGAGGAA CACAATCACT TCCTTCAGCC TGTCATGCGT 
AACAGTTTGT ACCTGCGGAG TGCCAGTGTG CCCACTGAAA ATATTCAGCT CAGATGCCCG 
CCAGTTGACA TCAACGGCTG TCGCAGACCC TCAGTCCTGC GCAAACCCTC CATCTCACAG 
ACCATCAAGA GGGGCACAGC AGACTGGTTT GGGGTCAGCA ATGATTTGGA TGCCACCCAG 
AAATGGCAGC GGAAGAGTTT GCGTCACTGC ACCCAGCGCT ACGGCAAACT AAAGCCTCAG 
GTGATCCGAG AGATGGACCT GCCCAGCCAA GACAATCTCT CCATGACCAG CACCGAAACC 
CCTCCTCCTC TATATGTCCC ACCGCCACAT CATGGCATGC AGAGGATCGT AGACCCGCTG 
GCTCGAGGTC GAGCGTTCCG TAATGTGGAT GAGGTGGATG GTGTGAATGT ACCTCAGACC 
CCCATCACCC CCTGCGCCGC TTCCCTGTGC TCCTTCACCA GCTCCCGCTC CGGCTTCAAC 
CGGCCTCCCC GCAGACGCAA GCGTGAGTCT GTCGCCAAGA TGGGCTTCAG AGCTGCTGCT 
GCTCTGGTCA AGGGTCGTTC TCTGCGGGAC GGGGCTGTTC GCCGATTGCC GAGGCGCAGT 
TTTGCCGCTG CTAGTTTCCT GGATGAGGAC ACAGTGGATT TCCCTGATGA ACTGGACACC 
TCTTTCTTCG CCAAGGATGG GGTTACCCAT GAAGAGCTGT CCACATACGC AGACGAGGTG 
TTTGAATCAC CTTCTGAAGC TGCCATTAAA GAAACAGATG CCAGTCGAGC GGGAGATGAG 
CGGGACCTGA CGGGGAGTGC CCTGGAGAAG ACCGAGCTGG AGCGGAGCCA TCTGATGCTG 
CCTCTGGAGA GGGGTTGGCG TAAAGCAAAG GATGGTGCTC TGGTTCAGCC TAAAGTGCGC 
TTACGGCAAG AGGTGGTGAG TGTGACTGGG CAGCGGCGTG GTCAGCGCAT TACTGTGCCT 
GTTAAAAAAC TCTTTGCTAA GGAAAAACGT CCTTATGGGC TTGGCATGGT GGGGAAACTG 
ACCAACCGCA CCTATCGCAA ACGCATTGAC AGCTATGTGA AGAAACAGAT CGAAGACATG 
AACGACCACA GGCCTTTCTT CACATATTGG ATCACCTTTG TTCATTTGTT GATTACCGTC 
TTGGCGGTTT CCATCTATGG CATTGCACCA GTGGGCTTTT CACAACATGA GACTGTTGAT 
TCTGTCTTAC GAAACAAAGG AGTTTATGAG AATGTCAGAT TCATGCAGCA AGAGAACTTC 
TGGGTTGGGC CAAGCTCGGA GGCGCTGATT CACTTGGGGG CGAAGTTTTC CCCCTGTATG 
CGACAGGATG AAGAGGTTCA CCAGCTCATC CAGCAGAAAA AGGCCAGCGA AAAACACTCG 
GCCTGCTGCG TCCGCAATGA CCAGTCCGGC TGCGTTCAGA CCTCTCAGGA GGAGTGTTCA 
AGCACTCTGG CATTATGGGT AAAGTGGCCT CAGCACCCAA GTGCTCCAGT TCTAGGGGAT 
AAAGTGCGTC AGTACGGCTC CGTGTGCCAT CAAGACCCCA GGATTTGTCT GGAGCCTGCC 
TCTGTTTCTC CTCATGTGTG GCCTGATGAC ATCAGCAAGT GGCCGATATG CACCAGGTAC 
AGCACAGGGA ATCACACTAA CCTGCCTCAC ATCGACTGCG TCATCACTGG TCGACCCTGC 
TGCATCGGAA CGAAAGGAAG GTGTGAGATT ACATCGAGAG AGTACTGTGA CTTCATGAAG 
GGATACTTCC ATGAGGAGGC CACGCTCTGT TCACAGGTTC ATTGCATGGA TGATGTTTGT 
GGCCTCCTGC CTTTTCTGAA CCCAGAGATC CCAGACCAGT TCTATCGACT CTGGCTATCT 
CTGTTTCTTC ACGCCGGGAT CCTGCACTGC CTGGTGTCTG TGTGCTTCCA GATGACCATT 
CTAAGGGATT TGGAGAAGCT GGCAGGCTGG CTGAGAATCT CTATCATCTA TATCCTCAGC 
GGGATTACGG GCAACCTCGC CAGTGCCATC TTCTTACCGT ACAGAGCAGA GGTGGGTCCG 
GCTGGCTCTC AGTTTGGCAT CTTGGCCTGT CTGTTCGTGG AGCTGTTCCA GAGCTGGCAG 
ATCTTGGCAC GGCCGTGGAG GGCCTTCACC AAGCTCTCAT GTGTAGTGCT GTTCCTTTTC 
GCCTTTGGCC TGCTGCCCTG GATAGATAAT TTCGCTCACA TCTGTGGCTT CGTCTCGGGC 
TTCTTCCTGT CCTTCGCCTT CCTGCCCTAC ATCAGCTTCG GCCGCATGGA CATGTACCGC 
AAACGTCTAC AGATCCTCGT GGCACTTACC CTTTTTGTGG GCATCTTCTC CAGCTTCGTG 
GTACTCTTCT ATGTTTATCC AGTTAAATGC GAGTGGTGTG AGTTTCTCAC CTGCATCCCG 
CTGACGGACA AGTTTTGTGA GAAATACGAC CTCAATGCTC ACCTCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304980.1
CDS	279..2849
Translation	MADAEDESRNDSLQRKKPPWLRLDIPTVEEHNHFLQPVMRNSLYLRSASVPTENIQLRCPPVDINGCRRPSVLRKPSISQTIKRGTADWFGVSNDLDATQKWQRKSLRHCTQRYGKLKPQVIREMDLPSQDNLSMTSTETPPPLYVPPPHHGMQRIVDPLARGRAFRNVDEVDGVNVPQTPITPCAASLCSFTSSRSGFNRPPRRRKRESVAKMGFRAAAALVKGRSLRDGAVRRLPRRSFAAASFLDEDTVDFPDELDTSFFAKDGVTHEELSTYADEVFESPSEAAIKETDASRAGDERDLTGSALEKTELERSHLMLPLERGWRKAKDGALVQPKVRLRQEVVSVTGQRRGQRITVPVKKLFAKEKRPYGLGMVGKLTNRTYRKRIDSYVKKQIEDMNDHRPFFTYWITFVHLLITVLAVSIYGIAPVGFSQHETVDSVLRNKGVYENVRFMQQENFWVGPSSEALIHLGAKFSPCMRQDEEVHQLIQQKKASEKHSACCVRNDQSGCVQTSQEECSSTLALWVKWPQHPSAPVLGDKVRQYGSVCHQDPRICLEPASVSPHVWPDDISKWPICTRYSTGNHTNLPHIDCVITGRPCCIGTKGRCEITSREYCDFMKGYFHEEATLCSQVHCMDDVCGLLPFLNPEIPDQFYRLWLSLFLHAGILHCLVSVCFQMTILRDLEKLAGWLRISIIYILSGITGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQILARPWRAFTKLSCVVLFLFAFGLLPWIDNFAHICGFVSGFFLSFAFLPYISFGRMDMYRKRLQILVALTLFVGIFSSFVVLFYVYPVKCEWCEFLTCIPLTDKFCEKYDLNAHLH

Target ORF information:

RefSeq Version	XM_009306705.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio rhomboid 5 homolog 1b (Drosophila) (rhbdf1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306705.2

ORF Insert Sequence:

ATGGCCGATG CTGAGGATGA GAGCAGGAAT GACAGCCTGC AGAGAAAGAA GCCGCCCTGG 
CTCAGACTGG ACATCCCCAC TGTGGAGGAA CACAATCACT TCCTTCAGCC TGTCATGCGT 
AACAGTTTGT ACCTGCGGAG TGCCAGTGTG CCCACTGAAA ATATTCAGCT CAGATGCCCG 
CCAGTTGACA TCAACGGCTG TCGCAGACCC TCAGTCCTGC GCAAACCCTC CATCTCACAG 
ACCATCAAGA GGGGCACAGC AGACTGGTTT GGGGTCAGCA ATGATTTGGA TGCCACCCAG 
AAATGGCAGC GGAAGAGTTT GCGTCACTGC ACCCAGCGCT ACGGCAAACT AAAGCCTCAG 
GTGATCCGAG AGATGGACCT GCCCAGCCAA GACAATCTCT CCATGACCAG CACCGAAACC 
CCTCCTCCTC TATATGTCCC ACCGCCACAT CATGGCATGC AGAGGATCGT AGACCCGCTG 
GCTCGAGGTC GAGCGTTCCG TAATGTGGAT GAGGTGGATG GTGTGAATGT ACCTCAGACC 
CCCATCACCC CCTGCGCCGC TTCCCTGTGC TCCTTCACCA GCTCCCGCTC CGGCTTCAAC 
CGGCCTCCCC GCAGACGCAA GCGTGAGTCT GTCGCCAAGA TGGGCTTCAG AGCTGCTGCT 
GCTCTGGTCA AGGGTCGTTC TCTGCGGGAC GGGGCTGTTC GCCGATTGCC GAGGCGCAGT 
TTTGCCGCTG CTAGTTTCCT GGATGAGGAC ACAGTGGATT TCCCTGATGA ACTGGACACC 
TCTTTCTTCG CCAAGGATGG GGTTACCCAT GAAGAGCTGT CCACATACGC AGACGAGGTG 
TTTGAATCAC CTTCTGAAGC TGCCATTAAA GAAACAGATG CCAGTCGAGC GGGAGATGAG 
CGGGACCTGA CGGGGAGTGC CCTGGAGAAG ACCGAGCTGG AGCGGAGCCA TCTGATGCTG 
CCTCTGGAGA GGGGTTGGCG TAAAGCAAAG GATGGTGCTC TGGTTCAGCC TAAAGTGCGC 
TTACGGCAAG AGGTGGTGAG TGTGACTGGG CAGCGGCGTG GTCAGCGCAT TACTGTGCCT 
GTTAAAAAAC TCTTTGCTAA GGAAAAACGT CCTTATGGGC TTGGCATGGT GGGGAAACTG 
ACCAACCGCA CCTATCGCAA ACGCATTGAC AGCTATGTGA AGAAACAGAT CGAAGACATG 
AACGACCACA GGCCTTTCTT CACATATTGG ATCACCTTTG TTCATTTGTT GATTACCGTC 
TTGGCGGTTT CCATCTATGG CATTGCACCA GTGGGCTTTT CACAACATGA GACTGTTGAT 
TCTGTCTTAC GAAACAAAGG AGTTTATGAG AATGTCAGAT TCATGCAGCA AGAGAACTTC 
TGGGTTGGGC CAAGCTCGGA GGCGCTGATT CACTTGGGGG CGAAGTTTTC CCCCTGTATG 
CGACAGGATG AAGAGGTTCA CCAGCTCATC CAGCAGAAAA AGGCCAGCGA AAAACACTCG 
GCCTGCTGCG TCCGCAATGA CCAGTCCGGC TGCGTTCAGA CCTCTCAGGA GGAGTGTTCA 
AGCACTCTGG CATTATGGGT AAAGTGGCCT CAGCACCCAA GTGCTCCAGT TCTAGGGGAT 
AAAGTGCGTC AGTACGGCTC CGTGTGCCAT CAAGACCCCA GGATTTGTCT GGAGCCTGCC 
TCTGTTTCTC CTCATGTGTG GCCTGATGAC ATCAGCAAGT GGCCGATATG CACCAGGTAC 
AGCACAGGGA ATCACACTAA CCTGCCTCAC ATCGACTGCG TCATCACTGG TCGACCCTGC 
TGCATCGGAA CGAAAGGAAG GTGTGAGATT ACATCGAGAG AGTACTGTGA CTTCATGAAG 
GGATACTTCC ATGAGGAGGC CACGCTCTGT TCACAGGTTC ATTGCATGGA TGATGTTTGT 
GGCCTCCTGC CTTTTCTGAA CCCAGAGATC CCAGACCAGT TCTATCGACT CTGGCTATCT 
CTGTTTCTTC ACGCCGGGAT CCTGCACTGC CTGGTGTCTG TGTGCTTCCA GATGACCATT 
CTAAGGGATT TGGAGAAGCT GGCAGGCTGG CTGAGAATCT CTATCATCTA TATCCTCAGC 
GGGATTACGG GCAACCTCGC CAGTGCCATC TTCTTACCGT ACAGAGCAGA GGTGGGTCCG 
GCTGGCTCTC AGTTTGGCAT CTTGGCCTGT CTGTTCGTGG AGCTGTTCCA GAGCTGGCAG 
ATCTTGGCAC GGCCGTGGAG GGCCTTCACC AAGCTCTCAT GTGTAGTGCT GTTCCTTTTC 
GCCTTTGGCC TGCTGCCCTG GATAGATAAT TTCGCTCACA TCTGTGGCTT CGTCTCGGGC 
TTCTTCCTGT CCTTCGCCTT CCTGCCCTAC ATCAGCTTCG GCCGCATGGA CATGTACCGC 
AAACGTCTAC AGATCCTCGT GGCACTTACC CTTTTTGTGG GCATCTTCTC CAGCTTCGTG 
GTACTCTTCT ATGTTTATCC AGTTAAATGC GAGTGGTGTG AGTTTCTCAC CTGCATCCCG 
CTGACGGACA AGTTTTGTGA GAAATACGAC CTCAATGCTC ACCTCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHBDF1B ( XM_021472526.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021328201.1 Danio rerio rhomboid 5 homolog 1b (Drosophila) (rhbdf1b), transcript variant X1, mRNA.

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CloneID	ODa145358
Clone ID Related Accession (Same CDS sequence)	XM_021472526.1
Accession Version	XM_021472526.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	inactive rhomboid protein 1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394837.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGATG CTGAGGATGA GAGCAGGAAT GACAGCCTGC AGAGAAAGAA GCCGCCCTGG 
CTCAGACTGG ATATCCCCAC TGTGGAGGAA CACAATCACT TCCTTCAGCC TGTTATGCGT 
AACAGTTTGT ACCTGCGGAG TGCCAGTGTG CCCACTGAAA ATATTCAGCT CAGATGCCCG 
CCAGTTGACA TCAACGGCTG TCGCAGACCC TCAGTCCTGC GTAAACCCTC CATCTCACAG 
ACCATCAAGA GTCCCACAGG GAAGAGAGTG CGCTTTGAGC AGATGAGCAC CGTCCTGGTT 
AAAGGCCACT GTGTCTCACG TCTGGAAGCC GACAGACGTC AGTCACTGCC CCAAATCGTC 
CTCAGGGGCA CAGCAGACTG GTTTGGGGTC AGCAATGATT TGGATGCCAC CCAGAAATGG 
CAGCGGAAGA GTTTGCGTCA CTGCACCCAG CGCTACGGCA AACTAAAGCC TCAGGTGATC 
CGAGAGATGG ACCTGCCCAG CCAAGACAAT CTCTCCATGA CCAGCACCGA AACCCCTCCT 
CCTCTATATG TCCCACCGCC ACATCATGGC ATGCAGAGGA TCGTAGACCC GCTGGCTCGA 
GGTCGAGCGT TCCGTAATGT GGATGAGGTG GATGGTGTGA ATGTACCTCA GACCCCCATC 
ACCCCCTGCG CCGCTTCCCT GTGCTCCTTC ACCAGCTCCC GCTCCGGCTT TAACCGGCCT 
CCCCGCAGAC GCAAGCGTGA GTCTGTCGCC AAGATGGGCT TCAGAGCTGC TGCTGCTCTG 
GTCAAGGGTC GTTCTCTGCG GGACGGGGCT GTTCGCCGAT TGCCGAGGCG CAGTTTTGCC 
GCTGCTAGTT TCCTGGATGA GGACACAGTG GATTTCCCTG ATGAACTGGA CACCTCTTTC 
TTCGCCAAGG ATGGGGTTAC CCATGAAGAG CTGTCCACAT ACACAGACGA GGTGTTTGAA 
TCACCTTCTG AAGCTGCCAT TAAAGAAACA GATGCCAGTC GAGCGGGAGA TGAGCGGGAC 
CTGACGGGGA GTGCCCTGGA GAAGACCGAG CTGGAGCGGA GCCATCTGAT GCTGCCTCTG 
GAGAGGGGTT GGCGTAAAGC AAAGGATGGT GCTCTGGTTC AGCCTAAAGT GCGCTTACGG 
CAAGAGGTGG TGAGTGTGAC TGGGCAGCGG CGTGGTCAGC GCATTACTGT GCCTGTTAAA 
AAACTCTTTG CTAAGGAAAA ACGTCCTTAT GGGCTTGGCA TGGTGGGGAA ACTGACCAAC 
CGCACCTATC GCAAACGCAT TGACAGCTAT GTGAAGAAAC AGATTGAAGA CATGAACGAC 
CACAGGCCTT TCTTCACATA TTGGATCACC TTTGTTCATT TGTTGATTAC CGTCTTGGCG 
GTTTCCATCT ATGGCATTGC ACCAGTGGGC TTTTCACAAC ATGAGACTGT TGATTCTGTC 
TTACGAAACA AAGGAGTTTA TGAGAATGTC AGATTCATGC AGCAAGAAAA CTTCTGGGTT 
GGGCCAAGCT CGGAGGCGCT GATTCACTTG GGGGCGAAGT TTTCCCCCTG TATGCGACAG 
GATGAAGAGG TTCACCAGCT CATCCAGCAG AAAAAGGCCA GCGAAAAACA CTCGGCCTGC 
TGCGTCCGCA ATGACCAGTC CGGCTGCGTT CAGACCTCTC AGGAGGAGTG TTCAAGCACT 
CTGGCATTAT GGGTAAAGTG GCCTCAGCAC CCAAGTGCTC CAGTTCTAGG GGATAAAGTG 
CGTCAGTACG GCTCCGTGTG CCATCAAGAC CCCAGGATTT GTCTGGAGCC TGCCTCTGTT 
TCTCCTCATG TGTGGCCTGA TGACATCAGC AAGTGGCCGA TATGCACCAG GTACAGCACA 
GGGAATCACA CTAACCTGCC TCACATCGAC TGCGTCATCA CTGGTCGACC CTGCTGCATC 
GGAACGAAAG GAAGGTGTGA GATTACATCG AGAGAGTACT GTGACTTCAT GAAGGGATAC 
TTCCATGAGG AGGCCACGCT CTGTTCACAG GTTCATTGCA TGGATGATGT TTGTGGCCTC 
CTGCCTTTTC TGAACCCAGA GATCCCAGAC CAGTTCTATC GACTCTGGCT ATCTCTGTTT 
CTTCACGCCG GGATCCTGCA CTGCCTGGTG TCTGTGTGCT TCCAGATGAC CATTCTAAGG 
GATTTGGAGA AGCTGGCAGG CTGGCTGAGA ATCTCTATCA TCTATATCCT CAGCGGGATT 
ACGGGCAACC TCGCCAGTGC CATCTTCTTA CCGTACAGAG CAGAGGTGGG TCCGGCTGGC 
TCTCAGTTTG GCATCTTGGC CTGTCTGTTC GTGGAGCTGT TCCAGAGCTG GCAGATCTTG 
GCACGGCCAT GGAGGGCCTT CACCAAGCTC TCATGTGTAG TGCTGTTCCT CTTCGCCTTT 
GGCCTGCTGC CCTGGATAGA TAATTTCGCT CACATCTGTG GCTTCGTCTC GGGCTTCTTC 
CTGTCCTTCG CCTTCCTGCC CTACATCAGC TTCGGCCGCA TGGACATGTA CCGCAAACGT 
CTACAGATCC TCGTGGCACT TACCCTTTTT GTGGGCATCT TCTCCAGCTT CGTGGTACTC 
TTCTATGTTT ATCCAGTTAA ATGCGAGTGG TGTGAGTTTC TCACCTGCAT CCCGCTGACG 
GACAAGTTTT GTGAGAAATA CGACCTCAAT GCTCACCTCC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328201.1
CDS	283..2967
Translation	MADAEDESRNDSLQRKKPPWLRLDIPTVEEHNHFLQPVMRNSLYLRSASVPTENIQLRCPPVDINGCRRPSVLRKPSISQTIKSPTGKRVRFEQMSTVLVKGHCVSRLEADRRQSLPQIVLRGTADWFGVSNDLDATQKWQRKSLRHCTQRYGKLKPQVIREMDLPSQDNLSMTSTETPPPLYVPPPHHGMQRIVDPLARGRAFRNVDEVDGVNVPQTPITPCAASLCSFTSSRSGFNRPPRRRKRESVAKMGFRAAAALVKGRSLRDGAVRRLPRRSFAAASFLDEDTVDFPDELDTSFFAKDGVTHEELSTYTDEVFESPSEAAIKETDASRAGDERDLTGSALEKTELERSHLMLPLERGWRKAKDGALVQPKVRLRQEVVSVTGQRRGQRITVPVKKLFAKEKRPYGLGMVGKLTNRTYRKRIDSYVKKQIEDMNDHRPFFTYWITFVHLLITVLAVSIYGIAPVGFSQHETVDSVLRNKGVYENVRFMQQENFWVGPSSEALIHLGAKFSPCMRQDEEVHQLIQQKKASEKHSACCVRNDQSGCVQTSQEECSSTLALWVKWPQHPSAPVLGDKVRQYGSVCHQDPRICLEPASVSPHVWPDDISKWPICTRYSTGNHTNLPHIDCVITGRPCCIGTKGRCEITSREYCDFMKGYFHEEATLCSQVHCMDDVCGLLPFLNPEIPDQFYRLWLSLFLHAGILHCLVSVCFQMTILRDLEKLAGWLRISIIYILSGITGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQILARPWRAFTKLSCVVLFLFAFGLLPWIDNFAHICGFVSGFFLSFAFLPYISFGRMDMYRKRLQILVALTLFVGIFSSFVVLFYVYPVKCEWCEFLTCIPLTDKFCEKYDLNAHLH

Target ORF information:

RefSeq Version	XM_021472526.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio rhomboid 5 homolog 1b (Drosophila) (rhbdf1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472526.1

ORF Insert Sequence:

ATGGCCGATG CTGAGGATGA GAGCAGGAAT GACAGCCTGC AGAGAAAGAA GCCGCCCTGG 
CTCAGACTGG ATATCCCCAC TGTGGAGGAA CACAATCACT TCCTTCAGCC TGTTATGCGT 
AACAGTTTGT ACCTGCGGAG TGCCAGTGTG CCCACTGAAA ATATTCAGCT CAGATGCCCG 
CCAGTTGACA TCAACGGCTG TCGCAGACCC TCAGTCCTGC GTAAACCCTC CATCTCACAG 
ACCATCAAGA GTCCCACAGG GAAGAGAGTG CGCTTTGAGC AGATGAGCAC CGTCCTGGTT 
AAAGGCCACT GTGTCTCACG TCTGGAAGCC GACAGACGTC AGTCACTGCC CCAAATCGTC 
CTCAGGGGCA CAGCAGACTG GTTTGGGGTC AGCAATGATT TGGATGCCAC CCAGAAATGG 
CAGCGGAAGA GTTTGCGTCA CTGCACCCAG CGCTACGGCA AACTAAAGCC TCAGGTGATC 
CGAGAGATGG ACCTGCCCAG CCAAGACAAT CTCTCCATGA CCAGCACCGA AACCCCTCCT 
CCTCTATATG TCCCACCGCC ACATCATGGC ATGCAGAGGA TCGTAGACCC GCTGGCTCGA 
GGTCGAGCGT TCCGTAATGT GGATGAGGTG GATGGTGTGA ATGTACCTCA GACCCCCATC 
ACCCCCTGCG CCGCTTCCCT GTGCTCCTTC ACCAGCTCCC GCTCCGGCTT TAACCGGCCT 
CCCCGCAGAC GCAAGCGTGA GTCTGTCGCC AAGATGGGCT TCAGAGCTGC TGCTGCTCTG 
GTCAAGGGTC GTTCTCTGCG GGACGGGGCT GTTCGCCGAT TGCCGAGGCG CAGTTTTGCC 
GCTGCTAGTT TCCTGGATGA GGACACAGTG GATTTCCCTG ATGAACTGGA CACCTCTTTC 
TTCGCCAAGG ATGGGGTTAC CCATGAAGAG CTGTCCACAT ACACAGACGA GGTGTTTGAA 
TCACCTTCTG AAGCTGCCAT TAAAGAAACA GATGCCAGTC GAGCGGGAGA TGAGCGGGAC 
CTGACGGGGA GTGCCCTGGA GAAGACCGAG CTGGAGCGGA GCCATCTGAT GCTGCCTCTG 
GAGAGGGGTT GGCGTAAAGC AAAGGATGGT GCTCTGGTTC AGCCTAAAGT GCGCTTACGG 
CAAGAGGTGG TGAGTGTGAC TGGGCAGCGG CGTGGTCAGC GCATTACTGT GCCTGTTAAA 
AAACTCTTTG CTAAGGAAAA ACGTCCTTAT GGGCTTGGCA TGGTGGGGAA ACTGACCAAC 
CGCACCTATC GCAAACGCAT TGACAGCTAT GTGAAGAAAC AGATTGAAGA CATGAACGAC 
CACAGGCCTT TCTTCACATA TTGGATCACC TTTGTTCATT TGTTGATTAC CGTCTTGGCG 
GTTTCCATCT ATGGCATTGC ACCAGTGGGC TTTTCACAAC ATGAGACTGT TGATTCTGTC 
TTACGAAACA AAGGAGTTTA TGAGAATGTC AGATTCATGC AGCAAGAAAA CTTCTGGGTT 
GGGCCAAGCT CGGAGGCGCT GATTCACTTG GGGGCGAAGT TTTCCCCCTG TATGCGACAG 
GATGAAGAGG TTCACCAGCT CATCCAGCAG AAAAAGGCCA GCGAAAAACA CTCGGCCTGC 
TGCGTCCGCA ATGACCAGTC CGGCTGCGTT CAGACCTCTC AGGAGGAGTG TTCAAGCACT 
CTGGCATTAT GGGTAAAGTG GCCTCAGCAC CCAAGTGCTC CAGTTCTAGG GGATAAAGTG 
CGTCAGTACG GCTCCGTGTG CCATCAAGAC CCCAGGATTT GTCTGGAGCC TGCCTCTGTT 
TCTCCTCATG TGTGGCCTGA TGACATCAGC AAGTGGCCGA TATGCACCAG GTACAGCACA 
GGGAATCACA CTAACCTGCC TCACATCGAC TGCGTCATCA CTGGTCGACC CTGCTGCATC 
GGAACGAAAG GAAGGTGTGA GATTACATCG AGAGAGTACT GTGACTTCAT GAAGGGATAC 
TTCCATGAGG AGGCCACGCT CTGTTCACAG GTTCATTGCA TGGATGATGT TTGTGGCCTC 
CTGCCTTTTC TGAACCCAGA GATCCCAGAC CAGTTCTATC GACTCTGGCT ATCTCTGTTT 
CTTCACGCCG GGATCCTGCA CTGCCTGGTG TCTGTGTGCT TCCAGATGAC CATTCTAAGG 
GATTTGGAGA AGCTGGCAGG CTGGCTGAGA ATCTCTATCA TCTATATCCT CAGCGGGATT 
ACGGGCAACC TCGCCAGTGC CATCTTCTTA CCGTACAGAG CAGAGGTGGG TCCGGCTGGC 
TCTCAGTTTG GCATCTTGGC CTGTCTGTTC GTGGAGCTGT TCCAGAGCTG GCAGATCTTG 
GCACGGCCAT GGAGGGCCTT CACCAAGCTC TCATGTGTAG TGCTGTTCCT CTTCGCCTTT 
GGCCTGCTGC CCTGGATAGA TAATTTCGCT CACATCTGTG GCTTCGTCTC GGGCTTCTTC 
CTGTCCTTCG CCTTCCTGCC CTACATCAGC TTCGGCCGCA TGGACATGTA CCGCAAACGT 
CTACAGATCC TCGTGGCACT TACCCTTTTT GTGGGCATCT TCTCCAGCTT CGTGGTACTC 
TTCTATGTTT ATCCAGTTAA ATGCGAGTGG TGTGAGTTTC TCACCTGCAT CCCGCTGACG 
GACAAGTTTT GTGAGAAATA CGACCTCAAT GCTCACCTCC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHBDF1B ( XM_021472527.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021328202.1 Danio rerio rhomboid 5 homolog 1b (Drosophila) (rhbdf1b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa145359
Clone ID Related Accession (Same CDS sequence)	XM_021472527.1
Accession Version	XM_021472527.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	inactive rhomboid protein 1-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394837.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGATG CTGAGGATGA GAGCAGGAAT GACAGCCTGC AGAGAAAGAA GCCGCCCTGG 
CTCAGACTGG ATATCCCCAC TGTGGAGGAA CACAATCACT TCCTTCAGCC TGTTATGCGT 
AACAGTTTGT ACCTGCGGAG TGCCAGTGTG CCCACTGAAA ATATTCAGCT CAGATGCCCG 
CCAGTTGACA TCAACGGCTG TCGCAGACCC TCAGTCCTGC GTAAACCCTC CATCTCACAG 
ACCATCAAGA GGGGCACAGC AGACTGGTTT GGGGTCAGCA ATGATTTGGA TGCCACCCAG 
AAATGGCAGC GGAAGAGTTT GCGTCACTGC ACCCAGCGCT ACGGCAAACT AAAGCCTCAG 
GTGATCCGAG AGATGGACCT GCCCAGCCAA GACAATCTCT CCATGACCAG CACCGAAACC 
CCTCCTCCTC TATATGTCCC ACCGCCACAT CATGGCATGC AGAGGATCGT AGACCCGCTG 
GCTCGAGGTC GAGCGTTCCG TAATGTGGAT GAGGTGGATG GTGTGAATGT ACCTCAGACC 
CCCATCACCC CCTGCGCCGC TTCCCTGTGC TCCTTCACCA GCTCCCGCTC CGGCTTTAAC 
CGGCCTCCCC GCAGACGCAA GCGTGAGTCT GTCGCCAAGA TGGGCTTCAG AGCTGCTGCT 
GCTCTGGTCA AGGGTCGTTC TCTGCGGGAC GGGGCTGTTC GCCGATTGCC GAGGCGCAGT 
TTTGCCGCTG CTAGTTTCCT GGATGAGGAC ACAGTGGATT TCCCTGATGA ACTGGACACC 
TCTTTCTTCG CCAAGGATGG GGTTACCCAT GAAGAGCTGT CCACATACAC AGACGAGGTG 
TTTGAATCAC CTTCTGAAGC TGCCATTAAA GAAACAGATG CCAGTCGAGC GGGAGATGAG 
CGGGACCTGA CGGGGAGTGC CCTGGAGAAG ACCGAGCTGG AGCGGAGCCA TCTGATGCTG 
CCTCTGGAGA GGGGTTGGCG TAAAGCAAAG GATGGTGCTC TGGTTCAGCC TAAAGTGCGC 
TTACGGCAAG AGGTGGTGAG TGTGACTGGG CAGCGGCGTG GTCAGCGCAT TACTGTGCCT 
GTTAAAAAAC TCTTTGCTAA GGAAAAACGT CCTTATGGGC TTGGCATGGT GGGGAAACTG 
ACCAACCGCA CCTATCGCAA ACGCATTGAC AGCTATGTGA AGAAACAGAT TGAAGACATG 
AACGACCACA GGCCTTTCTT CACATATTGG ATCACCTTTG TTCATTTGTT GATTACCGTC 
TTGGCGGTTT CCATCTATGG CATTGCACCA GTGGGCTTTT CACAACATGA GACTGTTGAT 
TCTGTCTTAC GAAACAAAGG AGTTTATGAG AATGTCAGAT TCATGCAGCA AGAAAACTTC 
TGGGTTGGGC CAAGCTCGGA GGCGCTGATT CACTTGGGGG CGAAGTTTTC CCCCTGTATG 
CGACAGGATG AAGAGGTTCA CCAGCTCATC CAGCAGAAAA AGGCCAGCGA AAAACACTCG 
GCCTGCTGCG TCCGCAATGA CCAGTCCGGC TGCGTTCAGA CCTCTCAGGA GGAGTGTTCA 
AGCACTCTGG CATTATGGGT AAAGTGGCCT CAGCACCCAA GTGCTCCAGT TCTAGGGGAT 
AAAGTGCGTC AGTACGGCTC CGTGTGCCAT CAAGACCCCA GGATTTGTCT GGAGCCTGCC 
TCTGTTTCTC CTCATGTGTG GCCTGATGAC ATCAGCAAGT GGCCGATATG CACCAGGTAC 
AGCACAGGGA ATCACACTAA CCTGCCTCAC ATCGACTGCG TCATCACTGG TCGACCCTGC 
TGCATCGGAA CGAAAGGAAG GTGTGAGATT ACATCGAGAG AGTACTGTGA CTTCATGAAG 
GGATACTTCC ATGAGGAGGC CACGCTCTGT TCACAGGTTC ATTGCATGGA TGATGTTTGT 
GGCCTCCTGC CTTTTCTGAA CCCAGAGATC CCAGACCAGT TCTATCGACT CTGGCTATCT 
CTGTTTCTTC ACGCCGGGAT CCTGCACTGC CTGGTGTCTG TGTGCTTCCA GATGACCATT 
CTAAGGGATT TGGAGAAGCT GGCAGGCTGG CTGAGAATCT CTATCATCTA TATCCTCAGC 
GGGATTACGG GCAACCTCGC CAGTGCCATC TTCTTACCGT ACAGAGCAGA GGTGGGTCCG 
GCTGGCTCTC AGTTTGGCAT CTTGGCCTGT CTGTTCGTGG AGCTGTTCCA GAGCTGGCAG 
ATCTTGGCAC GGCCATGGAG GGCCTTCACC AAGCTCTCAT GTGTAGTGCT GTTCCTCTTC 
GCCTTTGGCC TGCTGCCCTG GATAGATAAT TTCGCTCACA TCTGTGGCTT CGTCTCGGGC 
TTCTTCCTGT CCTTCGCCTT CCTGCCCTAC ATCAGCTTCG GCCGCATGGA CATGTACCGC 
AAACGTCTAC AGATCCTCGT GGCACTTACC CTTTTTGTGG GCATCTTCTC CAGCTTCGTG 
GTACTCTTCT ATGTTTATCC AGTTAAATGC GAGTGGTGTG AGTTTCTCAC CTGCATCCCG 
CTGACGGACA AGTTTTGTGA GAAATACGAC CTCAATGCTC ACCTCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328202.1
CDS	283..2853
Translation	MADAEDESRNDSLQRKKPPWLRLDIPTVEEHNHFLQPVMRNSLYLRSASVPTENIQLRCPPVDINGCRRPSVLRKPSISQTIKRGTADWFGVSNDLDATQKWQRKSLRHCTQRYGKLKPQVIREMDLPSQDNLSMTSTETPPPLYVPPPHHGMQRIVDPLARGRAFRNVDEVDGVNVPQTPITPCAASLCSFTSSRSGFNRPPRRRKRESVAKMGFRAAAALVKGRSLRDGAVRRLPRRSFAAASFLDEDTVDFPDELDTSFFAKDGVTHEELSTYTDEVFESPSEAAIKETDASRAGDERDLTGSALEKTELERSHLMLPLERGWRKAKDGALVQPKVRLRQEVVSVTGQRRGQRITVPVKKLFAKEKRPYGLGMVGKLTNRTYRKRIDSYVKKQIEDMNDHRPFFTYWITFVHLLITVLAVSIYGIAPVGFSQHETVDSVLRNKGVYENVRFMQQENFWVGPSSEALIHLGAKFSPCMRQDEEVHQLIQQKKASEKHSACCVRNDQSGCVQTSQEECSSTLALWVKWPQHPSAPVLGDKVRQYGSVCHQDPRICLEPASVSPHVWPDDISKWPICTRYSTGNHTNLPHIDCVITGRPCCIGTKGRCEITSREYCDFMKGYFHEEATLCSQVHCMDDVCGLLPFLNPEIPDQFYRLWLSLFLHAGILHCLVSVCFQMTILRDLEKLAGWLRISIIYILSGITGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQILARPWRAFTKLSCVVLFLFAFGLLPWIDNFAHICGFVSGFFLSFAFLPYISFGRMDMYRKRLQILVALTLFVGIFSSFVVLFYVYPVKCEWCEFLTCIPLTDKFCEKYDLNAHLH

Target ORF information:

RefSeq Version	XM_021472527.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio rhomboid 5 homolog 1b (Drosophila) (rhbdf1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472527.1

ORF Insert Sequence:

ATGGCCGATG CTGAGGATGA GAGCAGGAAT GACAGCCTGC AGAGAAAGAA GCCGCCCTGG 
CTCAGACTGG ATATCCCCAC TGTGGAGGAA CACAATCACT TCCTTCAGCC TGTTATGCGT 
AACAGTTTGT ACCTGCGGAG TGCCAGTGTG CCCACTGAAA ATATTCAGCT CAGATGCCCG 
CCAGTTGACA TCAACGGCTG TCGCAGACCC TCAGTCCTGC GTAAACCCTC CATCTCACAG 
ACCATCAAGA GGGGCACAGC AGACTGGTTT GGGGTCAGCA ATGATTTGGA TGCCACCCAG 
AAATGGCAGC GGAAGAGTTT GCGTCACTGC ACCCAGCGCT ACGGCAAACT AAAGCCTCAG 
GTGATCCGAG AGATGGACCT GCCCAGCCAA GACAATCTCT CCATGACCAG CACCGAAACC 
CCTCCTCCTC TATATGTCCC ACCGCCACAT CATGGCATGC AGAGGATCGT AGACCCGCTG 
GCTCGAGGTC GAGCGTTCCG TAATGTGGAT GAGGTGGATG GTGTGAATGT ACCTCAGACC 
CCCATCACCC CCTGCGCCGC TTCCCTGTGC TCCTTCACCA GCTCCCGCTC CGGCTTTAAC 
CGGCCTCCCC GCAGACGCAA GCGTGAGTCT GTCGCCAAGA TGGGCTTCAG AGCTGCTGCT 
GCTCTGGTCA AGGGTCGTTC TCTGCGGGAC GGGGCTGTTC GCCGATTGCC GAGGCGCAGT 
TTTGCCGCTG CTAGTTTCCT GGATGAGGAC ACAGTGGATT TCCCTGATGA ACTGGACACC 
TCTTTCTTCG CCAAGGATGG GGTTACCCAT GAAGAGCTGT CCACATACAC AGACGAGGTG 
TTTGAATCAC CTTCTGAAGC TGCCATTAAA GAAACAGATG CCAGTCGAGC GGGAGATGAG 
CGGGACCTGA CGGGGAGTGC CCTGGAGAAG ACCGAGCTGG AGCGGAGCCA TCTGATGCTG 
CCTCTGGAGA GGGGTTGGCG TAAAGCAAAG GATGGTGCTC TGGTTCAGCC TAAAGTGCGC 
TTACGGCAAG AGGTGGTGAG TGTGACTGGG CAGCGGCGTG GTCAGCGCAT TACTGTGCCT 
GTTAAAAAAC TCTTTGCTAA GGAAAAACGT CCTTATGGGC TTGGCATGGT GGGGAAACTG 
ACCAACCGCA CCTATCGCAA ACGCATTGAC AGCTATGTGA AGAAACAGAT TGAAGACATG 
AACGACCACA GGCCTTTCTT CACATATTGG ATCACCTTTG TTCATTTGTT GATTACCGTC 
TTGGCGGTTT CCATCTATGG CATTGCACCA GTGGGCTTTT CACAACATGA GACTGTTGAT 
TCTGTCTTAC GAAACAAAGG AGTTTATGAG AATGTCAGAT TCATGCAGCA AGAAAACTTC 
TGGGTTGGGC CAAGCTCGGA GGCGCTGATT CACTTGGGGG CGAAGTTTTC CCCCTGTATG 
CGACAGGATG AAGAGGTTCA CCAGCTCATC CAGCAGAAAA AGGCCAGCGA AAAACACTCG 
GCCTGCTGCG TCCGCAATGA CCAGTCCGGC TGCGTTCAGA CCTCTCAGGA GGAGTGTTCA 
AGCACTCTGG CATTATGGGT AAAGTGGCCT CAGCACCCAA GTGCTCCAGT TCTAGGGGAT 
AAAGTGCGTC AGTACGGCTC CGTGTGCCAT CAAGACCCCA GGATTTGTCT GGAGCCTGCC 
TCTGTTTCTC CTCATGTGTG GCCTGATGAC ATCAGCAAGT GGCCGATATG CACCAGGTAC 
AGCACAGGGA ATCACACTAA CCTGCCTCAC ATCGACTGCG TCATCACTGG TCGACCCTGC 
TGCATCGGAA CGAAAGGAAG GTGTGAGATT ACATCGAGAG AGTACTGTGA CTTCATGAAG 
GGATACTTCC ATGAGGAGGC CACGCTCTGT TCACAGGTTC ATTGCATGGA TGATGTTTGT 
GGCCTCCTGC CTTTTCTGAA CCCAGAGATC CCAGACCAGT TCTATCGACT CTGGCTATCT 
CTGTTTCTTC ACGCCGGGAT CCTGCACTGC CTGGTGTCTG TGTGCTTCCA GATGACCATT 
CTAAGGGATT TGGAGAAGCT GGCAGGCTGG CTGAGAATCT CTATCATCTA TATCCTCAGC 
GGGATTACGG GCAACCTCGC CAGTGCCATC TTCTTACCGT ACAGAGCAGA GGTGGGTCCG 
GCTGGCTCTC AGTTTGGCAT CTTGGCCTGT CTGTTCGTGG AGCTGTTCCA GAGCTGGCAG 
ATCTTGGCAC GGCCATGGAG GGCCTTCACC AAGCTCTCAT GTGTAGTGCT GTTCCTCTTC 
GCCTTTGGCC TGCTGCCCTG GATAGATAAT TTCGCTCACA TCTGTGGCTT CGTCTCGGGC 
TTCTTCCTGT CCTTCGCCTT CCTGCCCTAC ATCAGCTTCG GCCGCATGGA CATGTACCGC 
AAACGTCTAC AGATCCTCGT GGCACTTACC CTTTTTGTGG GCATCTTCTC CAGCTTCGTG 
GTACTCTTCT ATGTTTATCC AGTTAAATGC GAGTGGTGTG AGTTTCTCAC CTGCATCCCG 
CTGACGGACA AGTTTTGTGA GAAATACGAC CTCAATGCTC ACCTCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHBDF1B ( XM_021480300.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021335975.1 Danio rerio rhomboid 5 homolog 1b (Drosophila) (rhbdf1b), transcript variant X1, mRNA.

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CloneID	ODa148338
Clone ID Related Accession (Same CDS sequence)	XM_021480300.1
Accession Version	XM_021480300.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	inactive rhomboid protein 1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGATG CTGAGGATGA GAGCAGGAAT GACAGCCTGC AGAGAAAGAA GCCGCCCTGG 
CTCAGACTGG ACATCCCCAC TGTGGAGGAA CACAATCACT TCCTTCAGCC TGTCATGCGT 
AACAGTTTGT ACCTGCGGAG TGCCAGTGTG CCCACTGAAA ATATTCAGCT CAGATGCCCG 
CCAGTTGACA TCAACGGCTG TCGCAGACCC TCAGTCCTGC GCAAACCCTC CATCTCACAG 
ACCATCAAGA GTCCCACAGG GAAAAGAGTG CGCTTTGAGC AGATGAGCAC CGTCCTGGTT 
AAAGGCCACT GTGTCTCACG TCTGGAAGCC GACAGACGTC AGTCACTGCC CCAAATCGTC 
CTCAGGGGCA CAGCAGACTG GTTTGGGGTC AGCAATGATT TGGATGCCAC CCAGAAATGG 
CAGCGGAAGA GTTTGCGTCA CTGCACCCAG CGCTACGGCA AACTAAAGCC TCAGGTGATC 
CGAGAGATGG ACCTGCCCAG CCAAGACAAT CTCTCCATGA CCAGCACCGA AACCCCTCCT 
CCTCTATATG TCCCACCGCC ACATCATGGC ATGCAGAGGA TCGTAGACCC GCTGGCTCGA 
GGTCGAGCGT TCCGTAATGT GGATGAGGTG GATGGTGTGA ATGTACCTCA GACCCCCATC 
ACCCCCTGCG CCGCTTCCCT GTGCTCCTTC ACCAGCTCCC GCTCCGGCTT CAACCGGCCT 
CCCCGCAGAC GCAAGCGTGA GTCTGTCGCC AAGATGGGCT TCAGAGCTGC TGCTGCTCTG 
GTCAAGGGTC GTTCTCTGCG GGACGGGGCT GTTCGCCGAT TGCCGAGGCG CAGTTTTGCC 
GCTGCTAGTT TCCTGGATGA GGACACAGTG GATTTCCCTG ATGAACTGGA CACCTCTTTC 
TTCGCCAAGG ATGGGGTTAC CCATGAAGAG CTGTCCACAT ACGCAGACGA GGTGTTTGAA 
TCACCTTCTG AAGCTGCCAT TAAAGAAACA GATGCCAGTC GAGCGGGAGA TGAGCGGGAC 
CTGACGGGGA GTGCCCTGGA GAAGACCGAG CTGGAGCGGA GCCATCTGAT GCTGCCTCTG 
GAGAGGGGTT GGCGTAAAGC AAAGGATGGT GCTCTGGTTC AGCCTAAAGT GCGCTTACGG 
CAAGAGGTGG TGAGTGTGAC TGGGCAGCGG CGTGGTCAGC GCATTACTGT GCCTGTTAAA 
AAACTCTTTG CTAAGGAAAA ACGTCCTTAT GGGCTTGGCA TGGTGGGGAA ACTGACCAAC 
CGCACCTATC GCAAACGCAT TGACAGCTAT GTGAAGAAAC AGATCGAAGA CATGAACGAC 
CACAGGCCTT TCTTCACATA TTGGATCACC TTTGTTCATT TGTTGATTAC CGTCTTGGCG 
GTTTCCATCT ATGGCATTGC ACCAGTGGGC TTTTCACAAC ATGAGACTGT TGATTCTGTC 
TTACGAAACA AAGGAGTTTA TGAGAATGTC AGATTCATGC AGCAAGAGAA CTTCTGGGTT 
GGGCCAAGCT CGGAGGCGCT GATTCACTTG GGGGCGAAGT TTTCCCCCTG TATGCGACAG 
GATGAAGAGG TTCACCAGCT CATCCAGCAG AAAAAGGCCA GCGAAAAACA CTCGGCCTGC 
TGCGTCCGCA ATGACCAGTC CGGCTGCGTT CAGACCTCTC AGGAGGAGTG TTCAAGCACT 
CTGGCATTAT GGGTAAAGTG GCCTCAGCAC CCAAGTGCTC CAGTTCTAGG GGATAAAGTG 
CGTCAGTACG GCTCCGTGTG CCATCAAGAC CCCAGGATTT GTCTGGAGCC TGCCTCTGTT 
TCTCCTCATG TGTGGCCTGA TGACATCAGC AAGTGGCCGA TATGCACCAG GTACAGCACA 
GGGAATCACA CTAACCTGCC TCACATCGAC TGCGTCATCA CTGGTCGACC CTGCTGCATC 
GGAACGAAAG GAAGGTGTGA GATTACATCG AGAGAGTACT GTGACTTCAT GAAGGGATAC 
TTCCATGAGG AGGCCACGCT CTGTTCACAG GTTCATTGCA TGGATGATGT TTGTGGCCTC 
CTGCCTTTTC TGAACCCAGA GATCCCAGAC CAGTTCTATC GACTCTGGCT ATCTCTGTTT 
CTTCACGCCG GGATCCTGCA CTGCCTGGTG TCTGTGTGCT TCCAGATGAC CATTCTAAGG 
GATTTGGAGA AGCTGGCAGG CTGGCTGAGA ATCTCTATCA TCTATATCCT CAGCGGGATT 
ACGGGCAACC TCGCCAGTGC CATCTTCTTA CCGTACAGAG CAGAGGTGGG TCCGGCTGGC 
TCTCAGTTTG GCATCTTGGC CTGTCTGTTC GTGGAGCTGT TCCAGAGCTG GCAGATCTTG 
GCACGGCCGT GGAGGGCCTT CACCAAGCTC TCATGTGTAG TGCTGTTCCT TTTCGCCTTT 
GGCCTGCTGC CCTGGATAGA TAATTTCGCT CACATCTGTG GCTTCGTCTC GGGCTTCTTC 
CTGTCCTTCG CCTTCCTGCC CTACATCAGC TTCGGCCGCA TGGACATGTA CCGCAAACGT 
CTACAGATCC TCGTGGCACT TACCCTTTTT GTGGGCATCT TCTCCAGCTT CGTGGTACTC 
TTCTATGTTT ATCCAGTTAA ATGCGAGTGG TGTGAGTTTC TCACCTGCAT CCCGCTGACG 
GACAAGTTTT GTGAGAAATA CGACCTCAAT GCTCACCTCC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335975.1
CDS	286..2970
Translation	MADAEDESRNDSLQRKKPPWLRLDIPTVEEHNHFLQPVMRNSLYLRSASVPTENIQLRCPPVDINGCRRPSVLRKPSISQTIKSPTGKRVRFEQMSTVLVKGHCVSRLEADRRQSLPQIVLRGTADWFGVSNDLDATQKWQRKSLRHCTQRYGKLKPQVIREMDLPSQDNLSMTSTETPPPLYVPPPHHGMQRIVDPLARGRAFRNVDEVDGVNVPQTPITPCAASLCSFTSSRSGFNRPPRRRKRESVAKMGFRAAAALVKGRSLRDGAVRRLPRRSFAAASFLDEDTVDFPDELDTSFFAKDGVTHEELSTYADEVFESPSEAAIKETDASRAGDERDLTGSALEKTELERSHLMLPLERGWRKAKDGALVQPKVRLRQEVVSVTGQRRGQRITVPVKKLFAKEKRPYGLGMVGKLTNRTYRKRIDSYVKKQIEDMNDHRPFFTYWITFVHLLITVLAVSIYGIAPVGFSQHETVDSVLRNKGVYENVRFMQQENFWVGPSSEALIHLGAKFSPCMRQDEEVHQLIQQKKASEKHSACCVRNDQSGCVQTSQEECSSTLALWVKWPQHPSAPVLGDKVRQYGSVCHQDPRICLEPASVSPHVWPDDISKWPICTRYSTGNHTNLPHIDCVITGRPCCIGTKGRCEITSREYCDFMKGYFHEEATLCSQVHCMDDVCGLLPFLNPEIPDQFYRLWLSLFLHAGILHCLVSVCFQMTILRDLEKLAGWLRISIIYILSGITGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQILARPWRAFTKLSCVVLFLFAFGLLPWIDNFAHICGFVSGFFLSFAFLPYISFGRMDMYRKRLQILVALTLFVGIFSSFVVLFYVYPVKCEWCEFLTCIPLTDKFCEKYDLNAHLH

Target ORF information:

RefSeq Version	XM_021480300.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio rhomboid 5 homolog 1b (Drosophila) (rhbdf1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480300.1

ORF Insert Sequence:

ATGGCCGATG CTGAGGATGA GAGCAGGAAT GACAGCCTGC AGAGAAAGAA GCCGCCCTGG 
CTCAGACTGG ACATCCCCAC TGTGGAGGAA CACAATCACT TCCTTCAGCC TGTCATGCGT 
AACAGTTTGT ACCTGCGGAG TGCCAGTGTG CCCACTGAAA ATATTCAGCT CAGATGCCCG 
CCAGTTGACA TCAACGGCTG TCGCAGACCC TCAGTCCTGC GCAAACCCTC CATCTCACAG 
ACCATCAAGA GTCCCACAGG GAAAAGAGTG CGCTTTGAGC AGATGAGCAC CGTCCTGGTT 
AAAGGCCACT GTGTCTCACG TCTGGAAGCC GACAGACGTC AGTCACTGCC CCAAATCGTC 
CTCAGGGGCA CAGCAGACTG GTTTGGGGTC AGCAATGATT TGGATGCCAC CCAGAAATGG 
CAGCGGAAGA GTTTGCGTCA CTGCACCCAG CGCTACGGCA AACTAAAGCC TCAGGTGATC 
CGAGAGATGG ACCTGCCCAG CCAAGACAAT CTCTCCATGA CCAGCACCGA AACCCCTCCT 
CCTCTATATG TCCCACCGCC ACATCATGGC ATGCAGAGGA TCGTAGACCC GCTGGCTCGA 
GGTCGAGCGT TCCGTAATGT GGATGAGGTG GATGGTGTGA ATGTACCTCA GACCCCCATC 
ACCCCCTGCG CCGCTTCCCT GTGCTCCTTC ACCAGCTCCC GCTCCGGCTT CAACCGGCCT 
CCCCGCAGAC GCAAGCGTGA GTCTGTCGCC AAGATGGGCT TCAGAGCTGC TGCTGCTCTG 
GTCAAGGGTC GTTCTCTGCG GGACGGGGCT GTTCGCCGAT TGCCGAGGCG CAGTTTTGCC 
GCTGCTAGTT TCCTGGATGA GGACACAGTG GATTTCCCTG ATGAACTGGA CACCTCTTTC 
TTCGCCAAGG ATGGGGTTAC CCATGAAGAG CTGTCCACAT ACGCAGACGA GGTGTTTGAA 
TCACCTTCTG AAGCTGCCAT TAAAGAAACA GATGCCAGTC GAGCGGGAGA TGAGCGGGAC 
CTGACGGGGA GTGCCCTGGA GAAGACCGAG CTGGAGCGGA GCCATCTGAT GCTGCCTCTG 
GAGAGGGGTT GGCGTAAAGC AAAGGATGGT GCTCTGGTTC AGCCTAAAGT GCGCTTACGG 
CAAGAGGTGG TGAGTGTGAC TGGGCAGCGG CGTGGTCAGC GCATTACTGT GCCTGTTAAA 
AAACTCTTTG CTAAGGAAAA ACGTCCTTAT GGGCTTGGCA TGGTGGGGAA ACTGACCAAC 
CGCACCTATC GCAAACGCAT TGACAGCTAT GTGAAGAAAC AGATCGAAGA CATGAACGAC 
CACAGGCCTT TCTTCACATA TTGGATCACC TTTGTTCATT TGTTGATTAC CGTCTTGGCG 
GTTTCCATCT ATGGCATTGC ACCAGTGGGC TTTTCACAAC ATGAGACTGT TGATTCTGTC 
TTACGAAACA AAGGAGTTTA TGAGAATGTC AGATTCATGC AGCAAGAGAA CTTCTGGGTT 
GGGCCAAGCT CGGAGGCGCT GATTCACTTG GGGGCGAAGT TTTCCCCCTG TATGCGACAG 
GATGAAGAGG TTCACCAGCT CATCCAGCAG AAAAAGGCCA GCGAAAAACA CTCGGCCTGC 
TGCGTCCGCA ATGACCAGTC CGGCTGCGTT CAGACCTCTC AGGAGGAGTG TTCAAGCACT 
CTGGCATTAT GGGTAAAGTG GCCTCAGCAC CCAAGTGCTC CAGTTCTAGG GGATAAAGTG 
CGTCAGTACG GCTCCGTGTG CCATCAAGAC CCCAGGATTT GTCTGGAGCC TGCCTCTGTT 
TCTCCTCATG TGTGGCCTGA TGACATCAGC AAGTGGCCGA TATGCACCAG GTACAGCACA 
GGGAATCACA CTAACCTGCC TCACATCGAC TGCGTCATCA CTGGTCGACC CTGCTGCATC 
GGAACGAAAG GAAGGTGTGA GATTACATCG AGAGAGTACT GTGACTTCAT GAAGGGATAC 
TTCCATGAGG AGGCCACGCT CTGTTCACAG GTTCATTGCA TGGATGATGT TTGTGGCCTC 
CTGCCTTTTC TGAACCCAGA GATCCCAGAC CAGTTCTATC GACTCTGGCT ATCTCTGTTT 
CTTCACGCCG GGATCCTGCA CTGCCTGGTG TCTGTGTGCT TCCAGATGAC CATTCTAAGG 
GATTTGGAGA AGCTGGCAGG CTGGCTGAGA ATCTCTATCA TCTATATCCT CAGCGGGATT 
ACGGGCAACC TCGCCAGTGC CATCTTCTTA CCGTACAGAG CAGAGGTGGG TCCGGCTGGC 
TCTCAGTTTG GCATCTTGGC CTGTCTGTTC GTGGAGCTGT TCCAGAGCTG GCAGATCTTG 
GCACGGCCGT GGAGGGCCTT CACCAAGCTC TCATGTGTAG TGCTGTTCCT TTTCGCCTTT 
GGCCTGCTGC CCTGGATAGA TAATTTCGCT CACATCTGTG GCTTCGTCTC GGGCTTCTTC 
CTGTCCTTCG CCTTCCTGCC CTACATCAGC TTCGGCCGCA TGGACATGTA CCGCAAACGT 
CTACAGATCC TCGTGGCACT TACCCTTTTT GTGGGCATCT TCTCCAGCTT CGTGGTACTC 
TTCTATGTTT ATCCAGTTAA ATGCGAGTGG TGTGAGTTTC TCACCTGCAT CCCGCTGACG 
GACAAGTTTT GTGAGAAATA CGACCTCAAT GCTCACCTCC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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RHBDF1B cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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