cast cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	cast
Entrez Gene ID	563084
Full Name	calpastatin
Synonyms	sb:cb966,zgc:194249
General protein information	Preferred Names calpastatin Names calpastatin
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009295209.3	XP_009293484.1	calpastatin isoform X1
XM_005161025.4	XP_005161082.1	calpastatin isoform X2
XM_005161028.4	XP_005161085.1	calpastatin isoform X5
XM_005161034.4	XP_005161091.1	calpastatin isoform X13
XM_005161040.4	XP_005161097.1	calpastatin isoform X19
XM_005161046.4	XP_005161103.1	calpastatin isoform X27
XM_005161048.4	XP_005161105.1	calpastatin isoform X30
XM_017352805.1	XP_017208294.1	calpastatin isoform X17
XM_005161029.3	XP_005161086.1	calpastatin isoform X7
XM_005161049.3	XP_005161106.1	calpastatin isoform X25
NM_001130591.2	NP_001124063.2	calpastatin
XM_017352801.1	XP_017208290.1	calpastatin isoform X6
XM_017352803.1	XP_017208292.1	calpastatin isoform X12
XM_017352806.1	XP_017208295.1	calpastatin isoform X22
XM_005161028.3	XP_005161085.1	calpastatin isoform X5
XM_005161033.3	XP_005161090.1	calpastatin isoform X10
XM_005161040.3	XP_005161097.1	calpastatin isoform X18
XM_005161027.4	XP_005161084.1	calpastatin isoform X4
XM_017352801.2	XP_017208290.1	calpastatin isoform X6
XM_005161033.4	XP_005161090.1	calpastatin isoform X12
XM_005161036.4	XP_005161093.1	calpastatin isoform X15
XM_021468874.1	XP_021324549.1	calpastatin isoform X17
XM_021468881.1	XP_021324556.1	calpastatin isoform X29
XM_021468882.1	XP_021324557.1	calpastatin isoform X32
XM_005161034.3	XP_005161091.1	calpastatin isoform X11
XM_005161038.3	XP_005161095.1	calpastatin isoform X16
XM_005161042.3	XP_005161099.1	calpastatin isoform X20
XM_017352800.1	XP_017208289.1	calpastatin isoform X3
XM_017352808.1	XP_017208297.1	calpastatin isoform X26
XM_021468875.1	XP_021324550.1	calpastatin isoform X18
XM_021468876.1	XP_021324551.1	calpastatin isoform X20
XM_005161041.4	XP_005161098.1	calpastatin isoform X21
XM_005161043.4	XP_005161100.1	calpastatin isoform X23
XM_021468877.1	XP_021324552.1	calpastatin isoform X24
XM_021468879.1	XP_021324554.1	calpastatin isoform X26
XM_021468880.1	XP_021324555.1	calpastatin isoform X28
XM_021468869.1	XP_021324544.1	calpastatin isoform X7
XM_005161031.4	XP_005161088.1	calpastatin isoform X9
XM_017352804.1	XP_017208293.1	calpastatin isoform X13
XM_005161027.3	XP_005161084.1	calpastatin isoform X4
XM_005161036.3	XP_005161093.1	calpastatin isoform X14
XM_005161041.3	XP_005161098.1	calpastatin isoform X19
XM_017352802.2	XP_017208291.1	calpastatin isoform X8
XM_021468871.1	XP_021324546.1	calpastatin isoform X11
XM_021468872.1	XP_021324547.1	calpastatin isoform X14
XM_021468878.1	XP_021324553.1	calpastatin isoform X25
XM_017352808.2	XP_017208297.1	calpastatin isoform X34
XM_005161025.3	XP_005161082.1	calpastatin isoform X1
XM_009295209.2	XP_009293484.1	calpastatin isoform X2
XM_005161031.3	XP_005161088.1	calpastatin isoform X9
XM_005161037.3	XP_005161094.1	calpastatin isoform X15
XM_005161043.3	XP_005161100.1	calpastatin isoform X21
XM_005161046.3	XP_005161103.1	calpastatin isoform X23
XM_005161048.3	XP_005161105.1	calpastatin isoform X24
XM_017352802.1	XP_017208291.1	calpastatin isoform X8
XM_021468868.1	XP_021324543.1	calpastatin isoform X3
XM_021468870.1	XP_021324545.1	calpastatin isoform X10
XM_005161037.4	XP_005161094.1	calpastatin isoform X16
XM_005161042.4	XP_005161099.1	calpastatin isoform X22
XM_005161049.4	XP_005161106.1	calpastatin isoform X31
XM_021468883.1	XP_021324558.1	calpastatin isoform X33

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Functional characterization of zebrafish orthologs of the human Beta 3-Glucosyltransferase B3GLCT gene mutated in Peters Plus Syndrome.
Weh E, Takeuchi H, Muheisen S, Haltiwanger RS, Semina EV
PloS one12(9)e0184903(2017)

Ribosomal Proteins Rpl22 and Rpl22l1 Control Morphogenesis by Regulating Pre-mRNA Splicing.
Zhang Y, O'Leary MN, Peri S, Wang M, Zha J, Melov S, Kappes DJ, Feng Q, Rhodes J, Amieux PS, Morris DR, Kennedy BK, Wiest DL
Cell reports18(2)545-556(2017 01)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Phenotype anchoring in zebrafish reveals a potential role for matrix metalloproteinases (MMPs) in tamoxifen's effects on skin epithelium.
Bugel SM, Wehmas LC, La Du JK, Tanguay RL
Toxicology and applied pharmacology29631-41(2016 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following cast gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cast cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa37085	NM_001130591.2 Latest version!	Danio rerio calpastatin (cast), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00
ODa25603	XM_005161025.4 Latest version!	Danio rerio calpastatin (cast), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$510.30 $729.00
ODa25605	XM_005161027.4 Latest version!	Danio rerio calpastatin (cast), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$510.30 $729.00
ODa25606	XM_005161028.4 Latest version!	Danio rerio calpastatin (cast), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$510.30 $729.00
ODa25607	XM_005161029.3 Latest version!	Danio rerio calpastatin (cast), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$510.30 $729.00
ODa25609	XM_005161031.4 Latest version!	Danio rerio calpastatin (cast), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00
ODa25611	XM_005161033.4 Latest version!	Danio rerio calpastatin (cast), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00
ODa25611	XM_005161034.4 Latest version!	Danio rerio calpastatin (cast), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00
ODa25613	XM_005161036.4 Latest version!	Danio rerio calpastatin (cast), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00
ODa25614	XM_005161037.4 Latest version!	Danio rerio calpastatin (cast), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00
ODa25615	XM_005161038.3 Latest version!	Danio rerio calpastatin (cast), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00
ODa25617	XM_005161040.4 Latest version!	Danio rerio calpastatin (cast), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00
ODa25618	XM_005161041.4 Latest version!	Danio rerio calpastatin (cast), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00
ODa25619	XM_005161043.4 Latest version!	Danio rerio calpastatin (cast), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00
ODa25619	XM_005161042.4 Latest version!	Danio rerio calpastatin (cast), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00
ODa25622	XM_005161046.4 Latest version!	Danio rerio calpastatin (cast), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00
ODa25624	XM_005161049.4 Latest version!	Danio rerio calpastatin (cast), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00
ODa25624	XM_005161048.4 Latest version!	Danio rerio calpastatin (cast), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00
ODa43965	XM_009295209.3 Latest version!	Danio rerio calpastatin (cast), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$510.30 $729.00
ODa150920	XM_021468868.1 Latest version!	Danio rerio calpastatin (cast), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ODa150921	XM_021468869.1 Latest version!	Danio rerio calpastatin (cast), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ODa150922	XM_021468870.1 Latest version!	Danio rerio calpastatin (cast), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa150923	XM_021468871.1 Latest version!	Danio rerio calpastatin (cast), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa150924	XM_021468872.1 Latest version!	Danio rerio calpastatin (cast), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa150925	XM_021468874.1 Latest version!	Danio rerio calpastatin (cast), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa150926	XM_021468875.1 Latest version!	Danio rerio calpastatin (cast), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa150927	XM_021468876.1 Latest version!	Danio rerio calpastatin (cast), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa150928	XM_021468878.1 Latest version!	Danio rerio calpastatin (cast), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa150929	XM_021468879.1 Latest version!	Danio rerio calpastatin (cast), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa150930	XM_021468880.1 Latest version!	Danio rerio calpastatin (cast), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa25621	XM_021468877.1 Latest version!	Danio rerio calpastatin (cast), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa25623	XM_021468881.1 Latest version!	Danio rerio calpastatin (cast), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa25625	XM_021468882.1 Latest version!	Danio rerio calpastatin (cast), transcript variant X32, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa56796	XM_017352800.1 Latest version!	Danio rerio calpastatin (cast), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ODa56797	XM_017352801.2 Latest version!	Danio rerio calpastatin (cast), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ODa56798	XM_017352802.2 Latest version!	Danio rerio calpastatin (cast), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa56799	XM_017352803.1 Latest version!	Danio rerio calpastatin (cast), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa56800	XM_017352804.1 Latest version!	Danio rerio calpastatin (cast), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa56801	XM_017352805.1 Latest version!	Danio rerio calpastatin (cast), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa56802	XM_017352806.1 Latest version!	Danio rerio calpastatin (cast), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa56803	XM_017352808.2 Latest version!	Danio rerio calpastatin (cast), transcript variant X34, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa56803	XM_021468883.1 Latest version!	Danio rerio calpastatin (cast), transcript variant X33, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
ODa25606	XM_005161028.3	Danio rerio calpastatin (cast), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$510.30 $729.00
ODa25617	XM_005161040.3	Danio rerio calpastatin (cast), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00
ODa56797	XM_017352801.1	Danio rerio calpastatin (cast), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ODa25611	XM_005161033.3	Danio rerio calpastatin (cast), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00
ODa25611	XM_005161034.3	Danio rerio calpastatin (cast), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00
ODa25605	XM_005161027.3	Danio rerio calpastatin (cast), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$510.30 $729.00
ODa25613	XM_005161036.3	Danio rerio calpastatin (cast), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00
ODa25618	XM_005161041.3	Danio rerio calpastatin (cast), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00
ODa56803	XM_017352808.1	Danio rerio calpastatin (cast), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa25603	XM_005161025.3	Danio rerio calpastatin (cast), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$510.30 $729.00
ODa43965	XM_009295209.2	Danio rerio calpastatin (cast), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$510.30 $729.00
ODa25609	XM_005161031.3	Danio rerio calpastatin (cast), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00
ODa25619	XM_005161043.3	Danio rerio calpastatin (cast), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00
ODa25622	XM_005161046.3	Danio rerio calpastatin (cast), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00
ODa25624	XM_005161048.3	Danio rerio calpastatin (cast), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00
ODa56798	XM_017352802.1	Danio rerio calpastatin (cast), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa25614	XM_005161037.3	Danio rerio calpastatin (cast), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00
ODa25619	XM_005161042.3	Danio rerio calpastatin (cast), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00
ODa25624	XM_005161049.3	Danio rerio calpastatin (cast), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cast ( NM_001130591.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001124063.2 Danio rerio calpastatin (cast), mRNA.

Price & Availability↑

CloneID	ODa37085
Clone ID Related Accession (Same CDS sequence)	NM_001130591.2
Accession Version	NM_001130591.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	calpastatin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX511081.8, BX927307.12 and BX005379.9. On or before Jun 12, 2009 this sequence version replaced XM_686446.3, NM_001130591.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCGTACG CAATGTATTG GGCGCGCAAC AATGGAGACA CTTCATTTCA AAAACAAGAA 
TACGCTCCAC AGACATCAAG GTTCATGTCT CAGCAGGTCA CACCCACCAC AACATCTCAG 
GTCTCCACAG TGAAACCTGC ACAATATGAG AAAGGAGCTA CACAATCGTC TACAGCAGCT 
GCGACTGTCA AATCCGGCGC TGGAGATCCT GCAACCACCC AGAAAGGATC TTCTCAAGTC 
AAACCTCAGG TCACAGATTC TAAATCAACA CCTGCATCTT CTGCTAAAGT CTCAGCTGTG 
AGCTCTGGGA CTGGAGTTGC TGGTACAACC GGTGGAGCAG GAGTGAAACC AGCAGATCCT 
GCAAAAGATA CGCCTCAGAG CACCACCATT CCTTCAACCA AACTGGGACC TGCTAAGGTG 
GATCCGGCTG CTGGGAAGCC CTCTGGCTCA GCTGGTGCCC AAAAACAGAC AAGTGCTCAA 
AAGGTGCAGG TTGAAGTGGG TCCTTCAGCA GCAAAAGCTG ACGATCCGTT TGATGCCCTG 
TCTGGCACAT TACCATCATC ACAACCTTTG GCTCCTAAAG TCCACTACAC TGGACCTGAG 
ATCAAAGAGC CCAAACTGAA ATCAGAGAAA GGTGTTTTGT GTGGTGAGCG AGATGACACG 
CTGCCGCCCG GATACAGAAA GGAAGACCTG GAAAAGAAGC ATGGAGCTGG AGTTCCAGCT 
GGAGTTCCGG AGAAGCCTAA AGAAGTTCCC AAGCCCATAA GCACAGATGA TGCACTGGAA 
TCTCTCTCCT CTACGTTTGT GTCTCCAGCT CAAGCTGCTC CAAAGAAGAC AGACGTGAAA 
ACTGAAACAA TAGGAGCAGT GGATGTTAAA TCGGCAGGCA CCACTAACTT TGCTCCCCCT 
CCACCATCTC AGCAGAAACA ACCGGCGTCG TCTCAAGCTG CTGCTGTAAC CAAATCACCT 
GCTCCACCGG CAGACAAGAA AGCTAAAATG GAGACTCATG CACAACCAGC CAAAGCAAAG 
ACAGAGCTGG ACGCTCTCGA TGCTTTGAGC GACACACTGG GGGCTTCTAA ACCACCCCAA 
AAATTACCAG AACTCAAACC TGGACAGATA GTTGATGAGA AGAAACAGAC CTCAGAGAAA 
GGTGTTCGTG TTGGAGAAAG AGACGACACA CTTCCACCAC AATACAGATT CAAAGAGGAA 
GATCTCAAGA AATATCCTCC ACCTGCGAAA GAGCCTTCAC TGAACACAGA TGATGCGATG 
GACATACTTT CAGGAGATTT CACGACCCCT GCATCGGCAC CTGTTGTCAA AGCTCCTGTT 
CCTCCTGCAC AGGACACAAA GAAGCATGCT GCAGTGCCTG AGAAGACCAA AGATATTCCC 
AAGACTAAAG CTGATGCTCT GTCAAGTGAT TTCGTGGCTC CGGCACATTC TGCTCCTAAG 
GTTTCTTCAG CTGCTCCTAA AACCATCCCT CCAGGCCCAC CACAAACCCC AGATGAGGAT 
CCTTTCAGTG CTTTGGGCGA CACTCTTTCT GCCCCAGAAC CACCAAAAAA ACAACCTGAC 
CTGAAACCTA AGGACATCGT ACATGAGAAG GATGTGACGT CAAAGAAAGG AGTTCGTGTT 
GGAGAAAGAG ACGACACACT CCCACCAGAA TACAGATTCA AAGAGGAAGA TCTCAAGAAA 
TATCCTCCTC CTCAGAAAGA TCCTTCCTTA GATGCTAATG AAGCTCTGGA TATTCTGTCT 
GGGGATTTTA CAACTCCTTC CGCACCATCG GCTGTTTCCC CTGCCTCTAA ATCCTCTGCC 
AAGTCTTCAG ATTCAGCTTT AGATGCTCTT GCTGGTGATT TCGTCGCTTC TTCTTCTGCG 
TCTTCAGTTC ATTCTGCCGT CTCTGGCCCC CCACGCTCCG ACAGACTGTT GTCAGACGGA 
ACCTCATCAG CTCTAGATGC CCTGTCAGAA ACTCTGGGAG ACATTAAAGC AGCCCCTGAA 
CCCGCCCCTG TCCTACCTAA AACTGTCGTT AAGGAGAAGA CGGTCATAGA AGAGAGAGTG 
AGTAAACCGG GTGAGAGAGA CGACTCCCTT CCACCAGATC AGCGGTTCAC TGCGGAGGAT 
CGCAAGGCAT TTGAAGAAGC AAAGAAGAAA GACGCCAAAC CAAAACAGCC ATCAATGGAC 
GATTCAGCGG CTCTGGACAT GTTGTCTAGT GATTTTTCAG ATGTACCGAC TGTAAAGCCC 
ACTGTATCTG ATGGCAAAAG CTTCACCCCT GAACCAACAC CACCAACCTT TAAGGCAACC 
GGTCCGGTTT TAGACAAACT GGCAGACAAC GTGCTTTCCA ACCTGACTGA CCCCAAAGCC 
AAAGACAGCA AAGCAAAGAA ACAGGCAGTG GAAGACTCTT CAGCCACAGA TCTGCTGTCC 
AGTAAACCGA GCTCAGATGT GGTGCCTTCA GCTTCCACAA AGGCTGGAAA AAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124063.2
CDS	41..2497
Translation	MAYAMYWARNNGDTSFQKQEYAPQTSRFMSQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQVTDSKSTPASSAKVSAVSSGTGVAGTTGGAGVKPADPAKDTPQSTTIPSTKLGPAKVDPAAGKPSGSAGAQKQTSAQKVQVEVGPSAAKADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTELDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	NM_001130591.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpastatin (cast), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130591.2

ORF Insert Sequence:

ATGGCGTACG CAATGTATTG GGCGCGCAAC AATGGAGACA CTTCATTTCA AAAACAAGAA 
TACGCTCCAC AGACATCAAG GTTCATGTCT CAGCAGGTCA CACCCACCAC AACATCTCAG 
GTCTCCACAG TGAAACCTGC ACAATATGAG AAAGGAGCTA CACAATCGTC TACAGCAGCT 
GCGACTGTCA AATCCGGCGC TGGAGATCCT GCAACCACCC AGAAAGGATC TTCTCAAGTC 
AAACCTCAGG TCACAGATTC TAAATCAACA CCTGCATCTT CTGCTAAAGT CTCAGCTGTG 
AGCTCTGGGA CTGGAGTTGC TGGTACAACC GGTGGAGCAG GAGTGAAACC AGCAGATCCT 
GCAAAAGATA CGCCTCAGAG CACCACCATT CCTTCAACCA AACTGGGACC TGCTAAGGTG 
GATCCGGCTG CTGGGAAGCC CTCTGGCTCA GCTGGTGCCC AAAAACAGAC AAGTGCTCAA 
AAGGTGCAGG TTGAAGTGGG TCCTTCAGCA GCAAAAGCTG ACGATCCGTT TGATGCCCTG 
TCTGGCACAT TACCATCATC ACAACCTTTG GCTCCTAAAG TCCACTACAC TGGACCTGAG 
ATCAAAGAGC CCAAACTGAA ATCAGAGAAA GGTGTTTTGT GTGGTGAGCG AGATGACACG 
CTGCCGCCCG GATACAGAAA GGAAGACCTG GAAAAGAAGC ATGGAGCTGG AGTTCCAGCT 
GGAGTTCCGG AGAAGCCTAA AGAAGTTCCC AAGCCCATAA GCACAGATGA TGCACTGGAA 
TCTCTCTCCT CTACGTTTGT GTCTCCAGCT CAAGCTGCTC CAAAGAAGAC AGACGTGAAA 
ACTGAAACAA TAGGAGCAGT GGATGTTAAA TCGGCAGGCA CCACTAACTT TGCTCCCCCT 
CCACCATCTC AGCAGAAACA ACCGGCGTCG TCTCAAGCTG CTGCTGTAAC CAAATCACCT 
GCTCCACCGG CAGACAAGAA AGCTAAAATG GAGACTCATG CACAACCAGC CAAAGCAAAG 
ACAGAGCTGG ACGCTCTCGA TGCTTTGAGC GACACACTGG GGGCTTCTAA ACCACCCCAA 
AAATTACCAG AACTCAAACC TGGACAGATA GTTGATGAGA AGAAACAGAC CTCAGAGAAA 
GGTGTTCGTG TTGGAGAAAG AGACGACACA CTTCCACCAC AATACAGATT CAAAGAGGAA 
GATCTCAAGA AATATCCTCC ACCTGCGAAA GAGCCTTCAC TGAACACAGA TGATGCGATG 
GACATACTTT CAGGAGATTT CACGACCCCT GCATCGGCAC CTGTTGTCAA AGCTCCTGTT 
CCTCCTGCAC AGGACACAAA GAAGCATGCT GCAGTGCCTG AGAAGACCAA AGATATTCCC 
AAGACTAAAG CTGATGCTCT GTCAAGTGAT TTCGTGGCTC CGGCACATTC TGCTCCTAAG 
GTTTCTTCAG CTGCTCCTAA AACCATCCCT CCAGGCCCAC CACAAACCCC AGATGAGGAT 
CCTTTCAGTG CTTTGGGCGA CACTCTTTCT GCCCCAGAAC CACCAAAAAA ACAACCTGAC 
CTGAAACCTA AGGACATCGT ACATGAGAAG GATGTGACGT CAAAGAAAGG AGTTCGTGTT 
GGAGAAAGAG ACGACACACT CCCACCAGAA TACAGATTCA AAGAGGAAGA TCTCAAGAAA 
TATCCTCCTC CTCAGAAAGA TCCTTCCTTA GATGCTAATG AAGCTCTGGA TATTCTGTCT 
GGGGATTTTA CAACTCCTTC CGCACCATCG GCTGTTTCCC CTGCCTCTAA ATCCTCTGCC 
AAGTCTTCAG ATTCAGCTTT AGATGCTCTT GCTGGTGATT TCGTCGCTTC TTCTTCTGCG 
TCTTCAGTTC ATTCTGCCGT CTCTGGCCCC CCACGCTCCG ACAGACTGTT GTCAGACGGA 
ACCTCATCAG CTCTAGATGC CCTGTCAGAA ACTCTGGGAG ACATTAAAGC AGCCCCTGAA 
CCCGCCCCTG TCCTACCTAA AACTGTCGTT AAGGAGAAGA CGGTCATAGA AGAGAGAGTG 
AGTAAACCGG GTGAGAGAGA CGACTCCCTT CCACCAGATC AGCGGTTCAC TGCGGAGGAT 
CGCAAGGCAT TTGAAGAAGC AAAGAAGAAA GACGCCAAAC CAAAACAGCC ATCAATGGAC 
GATTCAGCGG CTCTGGACAT GTTGTCTAGT GATTTTTCAG ATGTACCGAC TGTAAAGCCC 
ACTGTATCTG ATGGCAAAAG CTTCACCCCT GAACCAACAC CACCAACCTT TAAGGCAACC 
GGTCCGGTTT TAGACAAACT GGCAGACAAC GTGCTTTCCA ACCTGACTGA CCCCAAAGCC 
AAAGACAGCA AAGCAAAGAA ACAGGCAGTG GAAGACTCTT CAGCCACAGA TCTGCTGTCC 
AGTAAACCGA GCTCAGATGT GGTGCCTTCA GCTTCCACAA AGGCTGGAAA AAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_005161025.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161082.1 Danio rerio calpastatin (cast), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa25603
Clone ID Related Accession (Same CDS sequence)	XM_005161025.4 , XM_005161025.3
Accession Version	XM_005161025.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calpastatin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005161025.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCACA AAAAGAGATG TCATGGTCGT AAGAAAAACA AAAAGGAGAA TGGAGACACT 
TCATTTCAAA AACAAGAATA CGCTCCACAG ACATCAAGGT TCATGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGAA AGGAGCTACA 
CAATCGTCTA CAGCAGCTGC GACTGTCAAA TCCGGCGCTG GAGATCCTGC AACCACCCAG 
AAAGGATCTT CTCAAGTCAA ACCTCAGGTC ACAGATTCTA AATCAACACC TGCATCTTCT 
GCTAAAGTCT CAGCTGTGAG CTCTGGGACT GGAGTTGCTG GTACAACCGG TGGAGCAGGA 
GTGAAACCAG CAGATCCTGC AAAAGATACG CCTCAGAGCA CCACCATTCC TTCAACCAAA 
CTGGGACCTG CTAAGGTGGA TCCGGCTGCT GGGAAGCCCT CTGGCTCAGC TGGTGCCCAA 
AAACAGACAA GTGCTCAAAA GGTGCAGGTT GAAGTGGGTC CTTCAGCAGC AAAAGTCAGT 
GCAGATGCTG ACGATCCGTT TGATGCCCTG TCTGGCACAT TACCATCATC ACAACCTTTG 
GCTCCTAAAG TCCACTACAC TGGACCTGAG ATCAAAGAGC CCAAACTGAA ATCAGAGAAA 
GGTGTTTTGT GTGGTGAGCG AGATGACACG CTGCCGCCCG GATACAGAAA GGAAGACCTG 
GAAAAGAAGC ATGGAGCTGG AGTTCCAGCT GGAGTTCCGG AGAAGCCTAA AGAAGTTCCC 
AAGCCCATAA GCACAGATGA TGCACTGGAA TCTCTCTCCT CTACGTTTGT GTCTCCAGCT 
CAAGCTGCTC CAAAGAAGAC AGACGTGAAA ACTGAAACAA TAGGAGCAGT GGATGTTAAA 
TCGGCAGGCA CCACTAACTT TGCTCCCCCT CCACCATCTC AGCAGAAACA ACCGGCGTCG 
TCTCAAGCTG CTGCTGTAAC CAAATCACCT GCTCCACCGG CAGACAAGAA AGCTAAAATG 
GAGACTCATG CACAACCAGC CAAAGCAAAG ACAGAGTCGG ACAGCTCCAT ATCGCTGGAC 
GCTCTCGATG CTTTGAGCGA CACACTGGGG GCTTCTAAAC CACCCCAAAA ATTACCAGAA 
CTCAAACCTG GACAGATAGT TGATGAGAAG AAACAGACCT CAGAGAAAGG TGTTCGTGTT 
GGAGAAAGAG ACGACACACT TCCACCACAA TACAGATTCA AAGAGGAAGA TCTCAAGAAA 
TATCCTCCAC CTGCGAAAGA GCCTTCACTG AACACAGATG ATGCGATGGA CATACTTTCA 
GGAGATTTCA CGACCCCTGC ATCGGCACCT GTTGTCAAAG CTCCTGTTCC TCCTGCACAG 
GACACAAAGA AGCATGCTGC AGTGCCTGAG AAGACCAAAG ATATTCCCAA GACTAAAGCT 
GATGCTCTGT CAAGTGATTT CGTGGCTCCG GCACATTCTG CTCCTAAGGT TTCTTCAGCT 
GCTCCTAAAA CCATCCCTCC AGGCCCACCA CAAACCCCAG ATGAGGATCC TTTCAGTGCT 
TTGGGCGACA CTCTTTCTGC CCCAGAACCA CCAAAAAAAC AACCTGACCT GAAACCTAAG 
GACATCGTAC ATGAGAAGGA TGTGACGTCA AAGAAAGGAG TTCGTGTTGG AGAAAGAGAC 
GACACACTCC CACCAGAATA CAGATTCAAA GAGGAAGATC TCAAGAAATA TCCTCCTCCT 
CAGAAAGATC CTTCCTTAGA TGCTAATGAA GCTCTGGATA TTCTGTCTGG GGATTTTACA 
ACTCCTTCCG CACCATCGGC TGTTTCCCCT GCCTCTAAAT CCTCTGCCAA GTCTTCAGAT 
TCAGCTTTAG ATGCTCTTGC TGGTGATTTC GTCGCTTCTT CTTCTGCGTC TTCAGTTCAT 
TCTGCCGTCT CTGGCCCCCC ACGCTCCGAC AGACTGTTGT CAGACGGAAC CTCATCAGCT 
CTAGATGCCC TGTCAGAAAC TCTGGGAGAC ATTAAAGCAG CCCCTGAACC CGCCCCTGTC 
CTACCTAAAA CTGTCGTTAA GGAGAAGACG GTCATAGAAG AGAGAGTGAG TAAACCGGGT 
GAGAGAGACG ACTCCCTTCC ACCAGATCAG CGGTTCACTG CGGAGGATCG CAAGGCATTT 
GAAGAAGCAA AGAAGAAAGA CGCCAAACCA AAACAGCCAT CAATGGACGA TTCAGCGGCT 
CTGGACATGT TGTCTAGTGA TTTTTCAGAT GTACCGACTG TAAAGCCCAC TGTATCTGAT 
GGCAAAAGCT TCACCCCTGA ACCAACACCA CCAACCTTTA AGGCAACCGG TCCGGTTTTA 
GACAAACTGG CAGACAACGT GCTTTCCAAC CTGACTGACC CCAAAGCCAA AGACAGCAAA 
GCAAAGGGAA AGGGGGCGAA ACCAAAGTCT AAACCAAAGA AACAGGCAGT GGAAGACTCT 
TCAGCCACAG ATCTGCTGTC CAGTAAACCG AGCTCAGATG TGGTGCCTTC AGCTTCCACA 
AAGGCTGGAA AAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161082.1
CDS	30..2567
Translation	MPHKKRCHGRKKNKKENGDTSFQKQEYAPQTSRFMSQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQVTDSKSTPASSAKVSAVSSGTGVAGTTGGAGVKPADPAKDTPQSTTIPSTKLGPAKVDPAAGKPSGSAGAQKQTSAQKVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_005161025.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161025.4

ORF Insert Sequence:

ATGCCGCACA AAAAGAGATG TCATGGTCGT AAGAAAAACA AAAAGGAGAA TGGAGACACT 
TCATTTCAAA AACAAGAATA CGCTCCACAG ACATCAAGGT TCATGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGAA AGGAGCTACA 
CAATCGTCTA CAGCAGCTGC GACTGTCAAA TCCGGCGCTG GAGATCCTGC AACCACCCAG 
AAAGGATCTT CTCAAGTCAA ACCTCAGGTC ACAGATTCTA AATCAACACC TGCATCTTCT 
GCTAAAGTCT CAGCTGTGAG CTCTGGGACT GGAGTTGCTG GTACAACCGG TGGAGCAGGA 
GTGAAACCAG CAGATCCTGC AAAAGATACG CCTCAGAGCA CCACCATTCC TTCAACCAAA 
CTGGGACCTG CTAAGGTGGA TCCGGCTGCT GGGAAGCCCT CTGGCTCAGC TGGTGCCCAA 
AAACAGACAA GTGCTCAAAA GGTGCAGGTT GAAGTGGGTC CTTCAGCAGC AAAAGTCAGT 
GCAGATGCTG ACGATCCGTT TGATGCCCTG TCTGGCACAT TACCATCATC ACAACCTTTG 
GCTCCTAAAG TCCACTACAC TGGACCTGAG ATCAAAGAGC CCAAACTGAA ATCAGAGAAA 
GGTGTTTTGT GTGGTGAGCG AGATGACACG CTGCCGCCCG GATACAGAAA GGAAGACCTG 
GAAAAGAAGC ATGGAGCTGG AGTTCCAGCT GGAGTTCCGG AGAAGCCTAA AGAAGTTCCC 
AAGCCCATAA GCACAGATGA TGCACTGGAA TCTCTCTCCT CTACGTTTGT GTCTCCAGCT 
CAAGCTGCTC CAAAGAAGAC AGACGTGAAA ACTGAAACAA TAGGAGCAGT GGATGTTAAA 
TCGGCAGGCA CCACTAACTT TGCTCCCCCT CCACCATCTC AGCAGAAACA ACCGGCGTCG 
TCTCAAGCTG CTGCTGTAAC CAAATCACCT GCTCCACCGG CAGACAAGAA AGCTAAAATG 
GAGACTCATG CACAACCAGC CAAAGCAAAG ACAGAGTCGG ACAGCTCCAT ATCGCTGGAC 
GCTCTCGATG CTTTGAGCGA CACACTGGGG GCTTCTAAAC CACCCCAAAA ATTACCAGAA 
CTCAAACCTG GACAGATAGT TGATGAGAAG AAACAGACCT CAGAGAAAGG TGTTCGTGTT 
GGAGAAAGAG ACGACACACT TCCACCACAA TACAGATTCA AAGAGGAAGA TCTCAAGAAA 
TATCCTCCAC CTGCGAAAGA GCCTTCACTG AACACAGATG ATGCGATGGA CATACTTTCA 
GGAGATTTCA CGACCCCTGC ATCGGCACCT GTTGTCAAAG CTCCTGTTCC TCCTGCACAG 
GACACAAAGA AGCATGCTGC AGTGCCTGAG AAGACCAAAG ATATTCCCAA GACTAAAGCT 
GATGCTCTGT CAAGTGATTT CGTGGCTCCG GCACATTCTG CTCCTAAGGT TTCTTCAGCT 
GCTCCTAAAA CCATCCCTCC AGGCCCACCA CAAACCCCAG ATGAGGATCC TTTCAGTGCT 
TTGGGCGACA CTCTTTCTGC CCCAGAACCA CCAAAAAAAC AACCTGACCT GAAACCTAAG 
GACATCGTAC ATGAGAAGGA TGTGACGTCA AAGAAAGGAG TTCGTGTTGG AGAAAGAGAC 
GACACACTCC CACCAGAATA CAGATTCAAA GAGGAAGATC TCAAGAAATA TCCTCCTCCT 
CAGAAAGATC CTTCCTTAGA TGCTAATGAA GCTCTGGATA TTCTGTCTGG GGATTTTACA 
ACTCCTTCCG CACCATCGGC TGTTTCCCCT GCCTCTAAAT CCTCTGCCAA GTCTTCAGAT 
TCAGCTTTAG ATGCTCTTGC TGGTGATTTC GTCGCTTCTT CTTCTGCGTC TTCAGTTCAT 
TCTGCCGTCT CTGGCCCCCC ACGCTCCGAC AGACTGTTGT CAGACGGAAC CTCATCAGCT 
CTAGATGCCC TGTCAGAAAC TCTGGGAGAC ATTAAAGCAG CCCCTGAACC CGCCCCTGTC 
CTACCTAAAA CTGTCGTTAA GGAGAAGACG GTCATAGAAG AGAGAGTGAG TAAACCGGGT 
GAGAGAGACG ACTCCCTTCC ACCAGATCAG CGGTTCACTG CGGAGGATCG CAAGGCATTT 
GAAGAAGCAA AGAAGAAAGA CGCCAAACCA AAACAGCCAT CAATGGACGA TTCAGCGGCT 
CTGGACATGT TGTCTAGTGA TTTTTCAGAT GTACCGACTG TAAAGCCCAC TGTATCTGAT 
GGCAAAAGCT TCACCCCTGA ACCAACACCA CCAACCTTTA AGGCAACCGG TCCGGTTTTA 
GACAAACTGG CAGACAACGT GCTTTCCAAC CTGACTGACC CCAAAGCCAA AGACAGCAAA 
GCAAAGGGAA AGGGGGCGAA ACCAAAGTCT AAACCAAAGA AACAGGCAGT GGAAGACTCT 
TCAGCCACAG ATCTGCTGTC CAGTAAACCG AGCTCAGATG TGGTGCCTTC AGCTTCCACA 
AAGGCTGGAA AAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_005161025.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161082.1 Danio rerio calpastatin (cast), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa25603
Clone ID Related Accession (Same CDS sequence)	XM_005161025.4 , XM_005161025.3
Accession Version	XM_005161025.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	calpastatin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005161025.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCACA AAAAGAGATG TCATGGTCGT AAGAAAAACA AAAAGGAGAA TGGAGACACT 
TCATTTCAAA AACAAGAATA CGCTCCACAG ACATCAAGGT TCATGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGAA AGGAGCTACA 
CAATCGTCTA CAGCAGCTGC GACTGTCAAA TCCGGCGCTG GAGATCCTGC AACCACCCAG 
AAAGGATCTT CTCAAGTCAA ACCTCAGGTC ACAGATTCTA AATCAACACC TGCATCTTCT 
GCTAAAGTCT CAGCTGTGAG CTCTGGGACT GGAGTTGCTG GTACAACCGG TGGAGCAGGA 
GTGAAACCAG CAGATCCTGC AAAAGATACG CCTCAGAGCA CCACCATTCC TTCAACCAAA 
CTGGGACCTG CTAAGGTGGA TCCGGCTGCT GGGAAGCCCT CTGGCTCAGC TGGTGCCCAA 
AAACAGACAA GTGCTCAAAA GGTGCAGGTT GAAGTGGGTC CTTCAGCAGC AAAAGTCAGT 
GCAGATGCTG ACGATCCGTT TGATGCCCTG TCTGGCACAT TACCATCATC ACAACCTTTG 
GCTCCTAAAG TCCACTACAC TGGACCTGAG ATCAAAGAGC CCAAACTGAA ATCAGAGAAA 
GGTGTTTTGT GTGGTGAGCG AGATGACACG CTGCCGCCCG GATACAGAAA GGAAGACCTG 
GAAAAGAAGC ATGGAGCTGG AGTTCCAGCT GGAGTTCCGG AGAAGCCTAA AGAAGTTCCC 
AAGCCCATAA GCACAGATGA TGCACTGGAA TCTCTCTCCT CTACGTTTGT GTCTCCAGCT 
CAAGCTGCTC CAAAGAAGAC AGACGTGAAA ACTGAAACAA TAGGAGCAGT GGATGTTAAA 
TCGGCAGGCA CCACTAACTT TGCTCCCCCT CCACCATCTC AGCAGAAACA ACCGGCGTCG 
TCTCAAGCTG CTGCTGTAAC CAAATCACCT GCTCCACCGG CAGACAAGAA AGCTAAAATG 
GAGACTCATG CACAACCAGC CAAAGCAAAG ACAGAGTCGG ACAGCTCCAT ATCGCTGGAC 
GCTCTCGATG CTTTGAGCGA CACACTGGGG GCTTCTAAAC CACCCCAAAA ATTACCAGAA 
CTCAAACCTG GACAGATAGT TGATGAGAAG AAACAGACCT CAGAGAAAGG TGTTCGTGTT 
GGAGAAAGAG ACGACACACT TCCACCACAA TACAGATTCA AAGAGGAAGA TCTCAAGAAA 
TATCCTCCAC CTGCGAAAGA GCCTTCACTG AACACAGATG ATGCGATGGA CATACTTTCA 
GGAGATTTCA CGACCCCTGC ATCGGCACCT GTTGTCAAAG CTCCTGTTCC TCCTGCACAG 
GACACAAAGA AGCATGCTGC AGTGCCTGAG AAGACCAAAG ATATTCCCAA GACTAAAGCT 
GATGCTCTGT CAAGTGATTT CGTGGCTCCG GCACATTCTG CTCCTAAGGT TTCTTCAGCT 
GCTCCTAAAA CCATCCCTCC AGGCCCACCA CAAACCCCAG ATGAGGATCC TTTCAGTGCT 
TTGGGCGACA CTCTTTCTGC CCCAGAACCA CCAAAAAAAC AACCTGACCT GAAACCTAAG 
GACATCGTAC ATGAGAAGGA TGTGACGTCA AAGAAAGGAG TTCGTGTTGG AGAAAGAGAC 
GACACACTCC CACCAGAATA CAGATTCAAA GAGGAAGATC TCAAGAAATA TCCTCCTCCT 
CAGAAAGATC CTTCCTTAGA TGCTAATGAA GCTCTGGATA TTCTGTCTGG GGATTTTACA 
ACTCCTTCCG CACCATCGGC TGTTTCCCCT GCCTCTAAAT CCTCTGCCAA GTCTTCAGAT 
TCAGCTTTAG ATGCTCTTGC TGGTGATTTC GTCGCTTCTT CTTCTGCGTC TTCAGTTCAT 
TCTGCCGTCT CTGGCCCCCC ACGCTCCGAC AGACTGTTGT CAGACGGAAC CTCATCAGCT 
CTAGATGCCC TGTCAGAAAC TCTGGGAGAC ATTAAAGCAG CCCCTGAACC CGCCCCTGTC 
CTACCTAAAA CTGTCGTTAA GGAGAAGACG GTCATAGAAG AGAGAGTGAG TAAACCGGGT 
GAGAGAGACG ACTCCCTTCC ACCAGATCAG CGGTTCACTG CGGAGGATCG CAAGGCATTT 
GAAGAAGCAA AGAAGAAAGA CGCCAAACCA AAACAGCCAT CAATGGACGA TTCAGCGGCT 
CTGGACATGT TGTCTAGTGA TTTTTCAGAT GTACCGACTG TAAAGCCCAC TGTATCTGAT 
GGCAAAAGCT TCACCCCTGA ACCAACACCA CCAACCTTTA AGGCAACCGG TCCGGTTTTA 
GACAAACTGG CAGACAACGT GCTTTCCAAC CTGACTGACC CCAAAGCCAA AGACAGCAAA 
GCAAAGGGAA AGGGGGCGAA ACCAAAGTCT AAACCAAAGA AACAGGCAGT GGAAGACTCT 
TCAGCCACAG ATCTGCTGTC CAGTAAACCG AGCTCAGATG TGGTGCCTTC AGCTTCCACA 
AAGGCTGGAA AAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161082.1
CDS	41..2578
Translation	MPHKKRCHGRKKNKKENGDTSFQKQEYAPQTSRFMSQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQVTDSKSTPASSAKVSAVSSGTGVAGTTGGAGVKPADPAKDTPQSTTIPSTKLGPAKVDPAAGKPSGSAGAQKQTSAQKVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_005161025.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161025.3

ORF Insert Sequence:

ATGCCGCACA AAAAGAGATG TCATGGTCGT AAGAAAAACA AAAAGGAGAA TGGAGACACT 
TCATTTCAAA AACAAGAATA CGCTCCACAG ACATCAAGGT TCATGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGAA AGGAGCTACA 
CAATCGTCTA CAGCAGCTGC GACTGTCAAA TCCGGCGCTG GAGATCCTGC AACCACCCAG 
AAAGGATCTT CTCAAGTCAA ACCTCAGGTC ACAGATTCTA AATCAACACC TGCATCTTCT 
GCTAAAGTCT CAGCTGTGAG CTCTGGGACT GGAGTTGCTG GTACAACCGG TGGAGCAGGA 
GTGAAACCAG CAGATCCTGC AAAAGATACG CCTCAGAGCA CCACCATTCC TTCAACCAAA 
CTGGGACCTG CTAAGGTGGA TCCGGCTGCT GGGAAGCCCT CTGGCTCAGC TGGTGCCCAA 
AAACAGACAA GTGCTCAAAA GGTGCAGGTT GAAGTGGGTC CTTCAGCAGC AAAAGTCAGT 
GCAGATGCTG ACGATCCGTT TGATGCCCTG TCTGGCACAT TACCATCATC ACAACCTTTG 
GCTCCTAAAG TCCACTACAC TGGACCTGAG ATCAAAGAGC CCAAACTGAA ATCAGAGAAA 
GGTGTTTTGT GTGGTGAGCG AGATGACACG CTGCCGCCCG GATACAGAAA GGAAGACCTG 
GAAAAGAAGC ATGGAGCTGG AGTTCCAGCT GGAGTTCCGG AGAAGCCTAA AGAAGTTCCC 
AAGCCCATAA GCACAGATGA TGCACTGGAA TCTCTCTCCT CTACGTTTGT GTCTCCAGCT 
CAAGCTGCTC CAAAGAAGAC AGACGTGAAA ACTGAAACAA TAGGAGCAGT GGATGTTAAA 
TCGGCAGGCA CCACTAACTT TGCTCCCCCT CCACCATCTC AGCAGAAACA ACCGGCGTCG 
TCTCAAGCTG CTGCTGTAAC CAAATCACCT GCTCCACCGG CAGACAAGAA AGCTAAAATG 
GAGACTCATG CACAACCAGC CAAAGCAAAG ACAGAGTCGG ACAGCTCCAT ATCGCTGGAC 
GCTCTCGATG CTTTGAGCGA CACACTGGGG GCTTCTAAAC CACCCCAAAA ATTACCAGAA 
CTCAAACCTG GACAGATAGT TGATGAGAAG AAACAGACCT CAGAGAAAGG TGTTCGTGTT 
GGAGAAAGAG ACGACACACT TCCACCACAA TACAGATTCA AAGAGGAAGA TCTCAAGAAA 
TATCCTCCAC CTGCGAAAGA GCCTTCACTG AACACAGATG ATGCGATGGA CATACTTTCA 
GGAGATTTCA CGACCCCTGC ATCGGCACCT GTTGTCAAAG CTCCTGTTCC TCCTGCACAG 
GACACAAAGA AGCATGCTGC AGTGCCTGAG AAGACCAAAG ATATTCCCAA GACTAAAGCT 
GATGCTCTGT CAAGTGATTT CGTGGCTCCG GCACATTCTG CTCCTAAGGT TTCTTCAGCT 
GCTCCTAAAA CCATCCCTCC AGGCCCACCA CAAACCCCAG ATGAGGATCC TTTCAGTGCT 
TTGGGCGACA CTCTTTCTGC CCCAGAACCA CCAAAAAAAC AACCTGACCT GAAACCTAAG 
GACATCGTAC ATGAGAAGGA TGTGACGTCA AAGAAAGGAG TTCGTGTTGG AGAAAGAGAC 
GACACACTCC CACCAGAATA CAGATTCAAA GAGGAAGATC TCAAGAAATA TCCTCCTCCT 
CAGAAAGATC CTTCCTTAGA TGCTAATGAA GCTCTGGATA TTCTGTCTGG GGATTTTACA 
ACTCCTTCCG CACCATCGGC TGTTTCCCCT GCCTCTAAAT CCTCTGCCAA GTCTTCAGAT 
TCAGCTTTAG ATGCTCTTGC TGGTGATTTC GTCGCTTCTT CTTCTGCGTC TTCAGTTCAT 
TCTGCCGTCT CTGGCCCCCC ACGCTCCGAC AGACTGTTGT CAGACGGAAC CTCATCAGCT 
CTAGATGCCC TGTCAGAAAC TCTGGGAGAC ATTAAAGCAG CCCCTGAACC CGCCCCTGTC 
CTACCTAAAA CTGTCGTTAA GGAGAAGACG GTCATAGAAG AGAGAGTGAG TAAACCGGGT 
GAGAGAGACG ACTCCCTTCC ACCAGATCAG CGGTTCACTG CGGAGGATCG CAAGGCATTT 
GAAGAAGCAA AGAAGAAAGA CGCCAAACCA AAACAGCCAT CAATGGACGA TTCAGCGGCT 
CTGGACATGT TGTCTAGTGA TTTTTCAGAT GTACCGACTG TAAAGCCCAC TGTATCTGAT 
GGCAAAAGCT TCACCCCTGA ACCAACACCA CCAACCTTTA AGGCAACCGG TCCGGTTTTA 
GACAAACTGG CAGACAACGT GCTTTCCAAC CTGACTGACC CCAAAGCCAA AGACAGCAAA 
GCAAAGGGAA AGGGGGCGAA ACCAAAGTCT AAACCAAAGA AACAGGCAGT GGAAGACTCT 
TCAGCCACAG ATCTGCTGTC CAGTAAACCG AGCTCAGATG TGGTGCCTTC AGCTTCCACA 
AAGGCTGGAA AAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_005161027.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161084.1 Danio rerio calpastatin (cast), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa25605
Clone ID Related Accession (Same CDS sequence)	XM_005161027.4 , XM_005161027.3
Accession Version	XM_005161027.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calpastatin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005161027.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTACG CAATGTATTG GGCGCGCAAC AATGGAGACA CTTCATTTCA AAAACAAGAA 
TACGCTCCAC AGACATCAAG GTTCATGTCT CAGCAGGTCA CACCCACCAC AACATCTCAG 
GTCTCCACAG TGAAACCTGC ACAATATGAG AAAGGAGCTA CACAATCGTC TACAGCAGCT 
GCGACTGTCA AATCCGGCGC TGGAGATCCT GCAACCACCC AGAAAGGATC TTCTCAAGTC 
AAACCTCAGG TCACAGATTC TAAATCAACA CCTGCATCTT CTGCTAAAGT CTCAGCTGTG 
AGCTCTGGGA CTGGAGTTGC TGGTACAACC GGTGGAGCAG GAGTGAAACC AGCAGATCCT 
GCAAAAGATA CGCCTCAGAG CACCACCATT CCTTCAACCA AACTGGGACC TGCTAAGGTG 
GATCCGGCTG CTGGGAAGCC CTCTGGCTCA GCTGGTGCCC AAAAACAGAC AAGTGCTCAA 
AAGGTGCAGG TTGAAGTGGG TCCTTCAGCA GCAAAAGTCA GTGCAGATGC TGACGATCCG 
TTTGATGCCC TGTCTGGCAC ATTACCATCA TCACAACCTT TGGCTCCTAA AGTCCACTAC 
ACTGGACCTG AGATCAAAGA GCCCAAACTG AAATCAGAGA AAGGTGTTTT GTGTGGTGAG 
CGAGATGACA CGCTGCCGCC CGGATACAGA AAGGAAGACC TGGAAAAGAA GCATGGAGCT 
GGAGTTCCAG CTGGAGTTCC GGAGAAGCCT AAAGAAGTTC CCAAGCCCAT AAGCACAGAT 
GATGCACTGG AATCTCTCTC CTCTACGTTT GTGTCTCCAG CTCAAGCTGC TCCAAAGAAG 
ACAGACGTGA AAACTGAAAC AATAGGAGCA GTGGATGTTA AATCGGCAGG CACCACTAAC 
TTTGCTCCCC CTCCACCATC TCAGCAGAAA CAACCGGCGT CGTCTCAAGC TGCTGCTGTA 
ACCAAATCAC CTGCTCCACC GGCAGACAAG AAAGCTAAAA TGGAGACTCA TGCACAACCA 
GCCAAAGCAA AGACAGAGTC GGACAGCTCC ATATCGCTGG ACGCTCTCGA TGCTTTGAGC 
GACACACTGG GGGCTTCTAA ACCACCCCAA AAATTACCAG AACTCAAACC TGGACAGATA 
GTTGATGAGA AGAAACAGAC CTCAGAGAAA GGTGTTCGTG TTGGAGAAAG AGACGACACA 
CTTCCACCAC AATACAGATT CAAAGAGGAA GATCTCAAGA AATATCCTCC ACCTGCGAAA 
GAGCCTTCAC TGAACACAGA TGATGCGATG GACATACTTT CAGGAGATTT CACGACCCCT 
GCATCGGCAC CTGTTGTCAA AGCTCCTGTT CCTCCTGCAC AGGACACAAA GAAGCATGCT 
GCAGTGCCTG AGAAGACCAA AGATATTCCC AAGACTAAAG CTGATGCTCT GTCAAGTGAT 
TTCGTGGCTC CGGCACATTC TGCTCCTAAG GTTTCTTCAG CTGCTCCTAA AACCATCCCT 
CCAGGCCCAC CACAAACCCC AGATGAGGAT CCTTTCAGTG CTTTGGGCGA CACTCTTTCT 
GCCCCAGAAC CACCAAAAAA ACAACCTGAC CTGAAACCTA AGGACATCGT ACATGAGAAG 
GATGTGACGT CAAAGAAAGG AGTTCGTGTT GGAGAAAGAG ACGACACACT CCCACCAGAA 
TACAGATTCA AAGAGGAAGA TCTCAAGAAA TATCCTCCTC CTCAGAAAGA TCCTTCCTTA 
GATGCTAATG AAGCTCTGGA TATTCTGTCT GGGGATTTTA CAACTCCTTC CGCACCATCG 
GCTGTTTCCC CTGCCTCTAA ATCCTCTGCC AAGTCTTCAG ATTCAGCTTT AGATGCTCTT 
GCTGGTGATT TCGTCGCTTC TTCTTCTGCG TCTTCAGTTC ATTCTGCCGT CTCTGGCCCC 
CCACGCTCCG ACAGACTGTT GTCAGACGGA ACCTCATCAG CTCTAGATGC CCTGTCAGAA 
ACTCTGGGAG ACATTAAAGC AGCCCCTGAA CCCGCCCCTG TCCTACCTAA AACTGTCGTT 
AAGGAGAAGA CGGTCATAGA AGAGAGAGTG AGTAAACCGG GTGAGAGAGA CGACTCCCTT 
CCACCAGATC AGCGGTTCAC TGCGGAGGAT CGCAAGGCAT TTGAAGAAGC AAAGAAGAAA 
GACGCCAAAC CAAAACAGCC ATCAATGGAC GATTCAGCGG CTCTGGACAT GTTGTCTAGT 
GATTTTTCAG ATGTACCGAC TGTAAAGCCC ACTGTATCTG ATGGCAAAAG CTTCACCCCT 
GAACCAACAC CACCAACCTT TAAGGCAACC GGTCCGGTTT TAGACAAACT GGCAGACAAC 
GTGCTTTCCA ACCTGACTGA CCCCAAAGCC AAAGACAGCA AAGCAAAGGG AAAGGGGGCG 
AAACCAAAGT CTAAACCAAA GAAACAGGCA GTGGAAGACT CTTCAGCCAC AGATCTGCTG 
TCCAGTAAAC CGAGCTCAGA TGTGGTGCCT TCAGCTTCCA CAAAGGCTGG AAAAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161084.1
CDS	113..2632
Translation	MAYAMYWARNNGDTSFQKQEYAPQTSRFMSQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQVTDSKSTPASSAKVSAVSSGTGVAGTTGGAGVKPADPAKDTPQSTTIPSTKLGPAKVDPAAGKPSGSAGAQKQTSAQKVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_005161027.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161027.4

ORF Insert Sequence:

ATGGCGTACG CAATGTATTG GGCGCGCAAC AATGGAGACA CTTCATTTCA AAAACAAGAA 
TACGCTCCAC AGACATCAAG GTTCATGTCT CAGCAGGTCA CACCCACCAC AACATCTCAG 
GTCTCCACAG TGAAACCTGC ACAATATGAG AAAGGAGCTA CACAATCGTC TACAGCAGCT 
GCGACTGTCA AATCCGGCGC TGGAGATCCT GCAACCACCC AGAAAGGATC TTCTCAAGTC 
AAACCTCAGG TCACAGATTC TAAATCAACA CCTGCATCTT CTGCTAAAGT CTCAGCTGTG 
AGCTCTGGGA CTGGAGTTGC TGGTACAACC GGTGGAGCAG GAGTGAAACC AGCAGATCCT 
GCAAAAGATA CGCCTCAGAG CACCACCATT CCTTCAACCA AACTGGGACC TGCTAAGGTG 
GATCCGGCTG CTGGGAAGCC CTCTGGCTCA GCTGGTGCCC AAAAACAGAC AAGTGCTCAA 
AAGGTGCAGG TTGAAGTGGG TCCTTCAGCA GCAAAAGTCA GTGCAGATGC TGACGATCCG 
TTTGATGCCC TGTCTGGCAC ATTACCATCA TCACAACCTT TGGCTCCTAA AGTCCACTAC 
ACTGGACCTG AGATCAAAGA GCCCAAACTG AAATCAGAGA AAGGTGTTTT GTGTGGTGAG 
CGAGATGACA CGCTGCCGCC CGGATACAGA AAGGAAGACC TGGAAAAGAA GCATGGAGCT 
GGAGTTCCAG CTGGAGTTCC GGAGAAGCCT AAAGAAGTTC CCAAGCCCAT AAGCACAGAT 
GATGCACTGG AATCTCTCTC CTCTACGTTT GTGTCTCCAG CTCAAGCTGC TCCAAAGAAG 
ACAGACGTGA AAACTGAAAC AATAGGAGCA GTGGATGTTA AATCGGCAGG CACCACTAAC 
TTTGCTCCCC CTCCACCATC TCAGCAGAAA CAACCGGCGT CGTCTCAAGC TGCTGCTGTA 
ACCAAATCAC CTGCTCCACC GGCAGACAAG AAAGCTAAAA TGGAGACTCA TGCACAACCA 
GCCAAAGCAA AGACAGAGTC GGACAGCTCC ATATCGCTGG ACGCTCTCGA TGCTTTGAGC 
GACACACTGG GGGCTTCTAA ACCACCCCAA AAATTACCAG AACTCAAACC TGGACAGATA 
GTTGATGAGA AGAAACAGAC CTCAGAGAAA GGTGTTCGTG TTGGAGAAAG AGACGACACA 
CTTCCACCAC AATACAGATT CAAAGAGGAA GATCTCAAGA AATATCCTCC ACCTGCGAAA 
GAGCCTTCAC TGAACACAGA TGATGCGATG GACATACTTT CAGGAGATTT CACGACCCCT 
GCATCGGCAC CTGTTGTCAA AGCTCCTGTT CCTCCTGCAC AGGACACAAA GAAGCATGCT 
GCAGTGCCTG AGAAGACCAA AGATATTCCC AAGACTAAAG CTGATGCTCT GTCAAGTGAT 
TTCGTGGCTC CGGCACATTC TGCTCCTAAG GTTTCTTCAG CTGCTCCTAA AACCATCCCT 
CCAGGCCCAC CACAAACCCC AGATGAGGAT CCTTTCAGTG CTTTGGGCGA CACTCTTTCT 
GCCCCAGAAC CACCAAAAAA ACAACCTGAC CTGAAACCTA AGGACATCGT ACATGAGAAG 
GATGTGACGT CAAAGAAAGG AGTTCGTGTT GGAGAAAGAG ACGACACACT CCCACCAGAA 
TACAGATTCA AAGAGGAAGA TCTCAAGAAA TATCCTCCTC CTCAGAAAGA TCCTTCCTTA 
GATGCTAATG AAGCTCTGGA TATTCTGTCT GGGGATTTTA CAACTCCTTC CGCACCATCG 
GCTGTTTCCC CTGCCTCTAA ATCCTCTGCC AAGTCTTCAG ATTCAGCTTT AGATGCTCTT 
GCTGGTGATT TCGTCGCTTC TTCTTCTGCG TCTTCAGTTC ATTCTGCCGT CTCTGGCCCC 
CCACGCTCCG ACAGACTGTT GTCAGACGGA ACCTCATCAG CTCTAGATGC CCTGTCAGAA 
ACTCTGGGAG ACATTAAAGC AGCCCCTGAA CCCGCCCCTG TCCTACCTAA AACTGTCGTT 
AAGGAGAAGA CGGTCATAGA AGAGAGAGTG AGTAAACCGG GTGAGAGAGA CGACTCCCTT 
CCACCAGATC AGCGGTTCAC TGCGGAGGAT CGCAAGGCAT TTGAAGAAGC AAAGAAGAAA 
GACGCCAAAC CAAAACAGCC ATCAATGGAC GATTCAGCGG CTCTGGACAT GTTGTCTAGT 
GATTTTTCAG ATGTACCGAC TGTAAAGCCC ACTGTATCTG ATGGCAAAAG CTTCACCCCT 
GAACCAACAC CACCAACCTT TAAGGCAACC GGTCCGGTTT TAGACAAACT GGCAGACAAC 
GTGCTTTCCA ACCTGACTGA CCCCAAAGCC AAAGACAGCA AAGCAAAGGG AAAGGGGGCG 
AAACCAAAGT CTAAACCAAA GAAACAGGCA GTGGAAGACT CTTCAGCCAC AGATCTGCTG 
TCCAGTAAAC CGAGCTCAGA TGTGGTGCCT TCAGCTTCCA CAAAGGCTGG AAAAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_005161027.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161084.1 Danio rerio calpastatin (cast), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa25605
Clone ID Related Accession (Same CDS sequence)	XM_005161027.4 , XM_005161027.3
Accession Version	XM_005161027.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	calpastatin isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005161027.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTACG CAATGTATTG GGCGCGCAAC AATGGAGACA CTTCATTTCA AAAACAAGAA 
TACGCTCCAC AGACATCAAG GTTCATGTCT CAGCAGGTCA CACCCACCAC AACATCTCAG 
GTCTCCACAG TGAAACCTGC ACAATATGAG AAAGGAGCTA CACAATCGTC TACAGCAGCT 
GCGACTGTCA AATCCGGCGC TGGAGATCCT GCAACCACCC AGAAAGGATC TTCTCAAGTC 
AAACCTCAGG TCACAGATTC TAAATCAACA CCTGCATCTT CTGCTAAAGT CTCAGCTGTG 
AGCTCTGGGA CTGGAGTTGC TGGTACAACC GGTGGAGCAG GAGTGAAACC AGCAGATCCT 
GCAAAAGATA CGCCTCAGAG CACCACCATT CCTTCAACCA AACTGGGACC TGCTAAGGTG 
GATCCGGCTG CTGGGAAGCC CTCTGGCTCA GCTGGTGCCC AAAAACAGAC AAGTGCTCAA 
AAGGTGCAGG TTGAAGTGGG TCCTTCAGCA GCAAAAGTCA GTGCAGATGC TGACGATCCG 
TTTGATGCCC TGTCTGGCAC ATTACCATCA TCACAACCTT TGGCTCCTAA AGTCCACTAC 
ACTGGACCTG AGATCAAAGA GCCCAAACTG AAATCAGAGA AAGGTGTTTT GTGTGGTGAG 
CGAGATGACA CGCTGCCGCC CGGATACAGA AAGGAAGACC TGGAAAAGAA GCATGGAGCT 
GGAGTTCCAG CTGGAGTTCC GGAGAAGCCT AAAGAAGTTC CCAAGCCCAT AAGCACAGAT 
GATGCACTGG AATCTCTCTC CTCTACGTTT GTGTCTCCAG CTCAAGCTGC TCCAAAGAAG 
ACAGACGTGA AAACTGAAAC AATAGGAGCA GTGGATGTTA AATCGGCAGG CACCACTAAC 
TTTGCTCCCC CTCCACCATC TCAGCAGAAA CAACCGGCGT CGTCTCAAGC TGCTGCTGTA 
ACCAAATCAC CTGCTCCACC GGCAGACAAG AAAGCTAAAA TGGAGACTCA TGCACAACCA 
GCCAAAGCAA AGACAGAGTC GGACAGCTCC ATATCGCTGG ACGCTCTCGA TGCTTTGAGC 
GACACACTGG GGGCTTCTAA ACCACCCCAA AAATTACCAG AACTCAAACC TGGACAGATA 
GTTGATGAGA AGAAACAGAC CTCAGAGAAA GGTGTTCGTG TTGGAGAAAG AGACGACACA 
CTTCCACCAC AATACAGATT CAAAGAGGAA GATCTCAAGA AATATCCTCC ACCTGCGAAA 
GAGCCTTCAC TGAACACAGA TGATGCGATG GACATACTTT CAGGAGATTT CACGACCCCT 
GCATCGGCAC CTGTTGTCAA AGCTCCTGTT CCTCCTGCAC AGGACACAAA GAAGCATGCT 
GCAGTGCCTG AGAAGACCAA AGATATTCCC AAGACTAAAG CTGATGCTCT GTCAAGTGAT 
TTCGTGGCTC CGGCACATTC TGCTCCTAAG GTTTCTTCAG CTGCTCCTAA AACCATCCCT 
CCAGGCCCAC CACAAACCCC AGATGAGGAT CCTTTCAGTG CTTTGGGCGA CACTCTTTCT 
GCCCCAGAAC CACCAAAAAA ACAACCTGAC CTGAAACCTA AGGACATCGT ACATGAGAAG 
GATGTGACGT CAAAGAAAGG AGTTCGTGTT GGAGAAAGAG ACGACACACT CCCACCAGAA 
TACAGATTCA AAGAGGAAGA TCTCAAGAAA TATCCTCCTC CTCAGAAAGA TCCTTCCTTA 
GATGCTAATG AAGCTCTGGA TATTCTGTCT GGGGATTTTA CAACTCCTTC CGCACCATCG 
GCTGTTTCCC CTGCCTCTAA ATCCTCTGCC AAGTCTTCAG ATTCAGCTTT AGATGCTCTT 
GCTGGTGATT TCGTCGCTTC TTCTTCTGCG TCTTCAGTTC ATTCTGCCGT CTCTGGCCCC 
CCACGCTCCG ACAGACTGTT GTCAGACGGA ACCTCATCAG CTCTAGATGC CCTGTCAGAA 
ACTCTGGGAG ACATTAAAGC AGCCCCTGAA CCCGCCCCTG TCCTACCTAA AACTGTCGTT 
AAGGAGAAGA CGGTCATAGA AGAGAGAGTG AGTAAACCGG GTGAGAGAGA CGACTCCCTT 
CCACCAGATC AGCGGTTCAC TGCGGAGGAT CGCAAGGCAT TTGAAGAAGC AAAGAAGAAA 
GACGCCAAAC CAAAACAGCC ATCAATGGAC GATTCAGCGG CTCTGGACAT GTTGTCTAGT 
GATTTTTCAG ATGTACCGAC TGTAAAGCCC ACTGTATCTG ATGGCAAAAG CTTCACCCCT 
GAACCAACAC CACCAACCTT TAAGGCAACC GGTCCGGTTT TAGACAAACT GGCAGACAAC 
GTGCTTTCCA ACCTGACTGA CCCCAAAGCC AAAGACAGCA AAGCAAAGGG AAAGGGGGCG 
AAACCAAAGT CTAAACCAAA GAAACAGGCA GTGGAAGACT CTTCAGCCAC AGATCTGCTG 
TCCAGTAAAC CGAGCTCAGA TGTGGTGCCT TCAGCTTCCA CAAAGGCTGG AAAAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161084.1
CDS	529..3048
Translation	MAYAMYWARNNGDTSFQKQEYAPQTSRFMSQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQVTDSKSTPASSAKVSAVSSGTGVAGTTGGAGVKPADPAKDTPQSTTIPSTKLGPAKVDPAAGKPSGSAGAQKQTSAQKVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_005161027.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161027.3

ORF Insert Sequence:

ATGGCGTACG CAATGTATTG GGCGCGCAAC AATGGAGACA CTTCATTTCA AAAACAAGAA 
TACGCTCCAC AGACATCAAG GTTCATGTCT CAGCAGGTCA CACCCACCAC AACATCTCAG 
GTCTCCACAG TGAAACCTGC ACAATATGAG AAAGGAGCTA CACAATCGTC TACAGCAGCT 
GCGACTGTCA AATCCGGCGC TGGAGATCCT GCAACCACCC AGAAAGGATC TTCTCAAGTC 
AAACCTCAGG TCACAGATTC TAAATCAACA CCTGCATCTT CTGCTAAAGT CTCAGCTGTG 
AGCTCTGGGA CTGGAGTTGC TGGTACAACC GGTGGAGCAG GAGTGAAACC AGCAGATCCT 
GCAAAAGATA CGCCTCAGAG CACCACCATT CCTTCAACCA AACTGGGACC TGCTAAGGTG 
GATCCGGCTG CTGGGAAGCC CTCTGGCTCA GCTGGTGCCC AAAAACAGAC AAGTGCTCAA 
AAGGTGCAGG TTGAAGTGGG TCCTTCAGCA GCAAAAGTCA GTGCAGATGC TGACGATCCG 
TTTGATGCCC TGTCTGGCAC ATTACCATCA TCACAACCTT TGGCTCCTAA AGTCCACTAC 
ACTGGACCTG AGATCAAAGA GCCCAAACTG AAATCAGAGA AAGGTGTTTT GTGTGGTGAG 
CGAGATGACA CGCTGCCGCC CGGATACAGA AAGGAAGACC TGGAAAAGAA GCATGGAGCT 
GGAGTTCCAG CTGGAGTTCC GGAGAAGCCT AAAGAAGTTC CCAAGCCCAT AAGCACAGAT 
GATGCACTGG AATCTCTCTC CTCTACGTTT GTGTCTCCAG CTCAAGCTGC TCCAAAGAAG 
ACAGACGTGA AAACTGAAAC AATAGGAGCA GTGGATGTTA AATCGGCAGG CACCACTAAC 
TTTGCTCCCC CTCCACCATC TCAGCAGAAA CAACCGGCGT CGTCTCAAGC TGCTGCTGTA 
ACCAAATCAC CTGCTCCACC GGCAGACAAG AAAGCTAAAA TGGAGACTCA TGCACAACCA 
GCCAAAGCAA AGACAGAGTC GGACAGCTCC ATATCGCTGG ACGCTCTCGA TGCTTTGAGC 
GACACACTGG GGGCTTCTAA ACCACCCCAA AAATTACCAG AACTCAAACC TGGACAGATA 
GTTGATGAGA AGAAACAGAC CTCAGAGAAA GGTGTTCGTG TTGGAGAAAG AGACGACACA 
CTTCCACCAC AATACAGATT CAAAGAGGAA GATCTCAAGA AATATCCTCC ACCTGCGAAA 
GAGCCTTCAC TGAACACAGA TGATGCGATG GACATACTTT CAGGAGATTT CACGACCCCT 
GCATCGGCAC CTGTTGTCAA AGCTCCTGTT CCTCCTGCAC AGGACACAAA GAAGCATGCT 
GCAGTGCCTG AGAAGACCAA AGATATTCCC AAGACTAAAG CTGATGCTCT GTCAAGTGAT 
TTCGTGGCTC CGGCACATTC TGCTCCTAAG GTTTCTTCAG CTGCTCCTAA AACCATCCCT 
CCAGGCCCAC CACAAACCCC AGATGAGGAT CCTTTCAGTG CTTTGGGCGA CACTCTTTCT 
GCCCCAGAAC CACCAAAAAA ACAACCTGAC CTGAAACCTA AGGACATCGT ACATGAGAAG 
GATGTGACGT CAAAGAAAGG AGTTCGTGTT GGAGAAAGAG ACGACACACT CCCACCAGAA 
TACAGATTCA AAGAGGAAGA TCTCAAGAAA TATCCTCCTC CTCAGAAAGA TCCTTCCTTA 
GATGCTAATG AAGCTCTGGA TATTCTGTCT GGGGATTTTA CAACTCCTTC CGCACCATCG 
GCTGTTTCCC CTGCCTCTAA ATCCTCTGCC AAGTCTTCAG ATTCAGCTTT AGATGCTCTT 
GCTGGTGATT TCGTCGCTTC TTCTTCTGCG TCTTCAGTTC ATTCTGCCGT CTCTGGCCCC 
CCACGCTCCG ACAGACTGTT GTCAGACGGA ACCTCATCAG CTCTAGATGC CCTGTCAGAA 
ACTCTGGGAG ACATTAAAGC AGCCCCTGAA CCCGCCCCTG TCCTACCTAA AACTGTCGTT 
AAGGAGAAGA CGGTCATAGA AGAGAGAGTG AGTAAACCGG GTGAGAGAGA CGACTCCCTT 
CCACCAGATC AGCGGTTCAC TGCGGAGGAT CGCAAGGCAT TTGAAGAAGC AAAGAAGAAA 
GACGCCAAAC CAAAACAGCC ATCAATGGAC GATTCAGCGG CTCTGGACAT GTTGTCTAGT 
GATTTTTCAG ATGTACCGAC TGTAAAGCCC ACTGTATCTG ATGGCAAAAG CTTCACCCCT 
GAACCAACAC CACCAACCTT TAAGGCAACC GGTCCGGTTT TAGACAAACT GGCAGACAAC 
GTGCTTTCCA ACCTGACTGA CCCCAAAGCC AAAGACAGCA AAGCAAAGGG AAAGGGGGCG 
AAACCAAAGT CTAAACCAAA GAAACAGGCA GTGGAAGACT CTTCAGCCAC AGATCTGCTG 
TCCAGTAAAC CGAGCTCAGA TGTGGTGCCT TCAGCTTCCA CAAAGGCTGG AAAAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_005161028.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161085.1 Danio rerio calpastatin (cast), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa25606
Clone ID Related Accession (Same CDS sequence)	XM_005161028.4 , XM_005161028.3
Accession Version	XM_005161028.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calpastatin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005161028.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGTCTCA GCAGGTCACA CCCACCACAA CATCTCAGGT CTCCACAGTG 
AAACCTGCAC AATATGAGAA AGGAGCTACA CAATCGTCTA CAGCAGCTGC GACTGTCAAA 
TCCGGCGCTG GAGATCCTGC AACCACCCAG AAAGGATCTT CTCAAGTCAA ACCTCAGGTC 
ACAGATTCTA AATCAACACC TGCATCTTCT GCTAAAGTCT CAGCTGTGAG CTCTGGGACT 
GGAGTTGCTG GTACAACCGG TGGAGCAGGA GTGAAACCAG CAGATCCTGC AAAAGATACG 
CCTCAGAGCA CCACCATTCC TTCAACCAAA CTGGGACCTG CTAAGGTGGA TCCGGCTGCT 
GGGAAGCCCT CTGGCTCAGC TGGTGCCCAA AAACAGACAA GTGCTCAAAA GGTGCAGGTT 
GAAGTGGGTC CTTCAGCAGC AAAAGTCAGT GCAGATGCTG ACGATCCGTT TGATGCCCTG 
TCTGGCACAT TACCATCATC ACAACCTTTG GCTCCTAAAG TCCACTACAC TGGACCTGAG 
ATCAAAGAGC CCAAACTGAA ATCAGAGAAA GGTGTTTTGT GTGGTGAGCG AGATGACACG 
CTGCCGCCCG GATACAGAAA GGAAGACCTG GAAAAGAAGC ATGGAGCTGG AGTTCCAGCT 
GGAGTTCCGG AGAAGCCTAA AGAAGTTCCC AAGCCCATAA GCACAGATGA TGCACTGGAA 
TCTCTCTCCT CTACGTTTGT GTCTCCAGCT CAAGCTGCTC CAAAGAAGAC AGACGTGAAA 
ACTGAAACAA TAGGAGCAGT GGATGTTAAA TCGGCAGGCA CCACTAACTT TGCTCCCCCT 
CCACCATCTC AGCAGAAACA ACCGGCGTCG TCTCAAGCTG CTGCTGTAAC CAAATCACCT 
GCTCCACCGG CAGACAAGAA AGCTAAAATG GAGACTCATG CACAACCAGC CAAAGCAAAG 
ACAGAGTCGG ACAGCTCCAT ATCGCTGGAC GCTCTCGATG CTTTGAGCGA CACACTGGGG 
GCTTCTAAAC CACCCCAAAA ATTACCAGAA CTCAAACCTG GACAGATAGT TGATGAGAAG 
AAACAGACCT CAGAGAAAGG TGTTCGTGTT GGAGAAAGAG ACGACACACT TCCACCACAA 
TACAGATTCA AAGAGGAAGA TCTCAAGAAA TATCCTCCAC CTGCGAAAGA GCCTTCACTG 
AACACAGATG ATGCGATGGA CATACTTTCA GGAGATTTCA CGACCCCTGC ATCGGCACCT 
GTTGTCAAAG CTCCTGTTCC TCCTGCACAG GACACAAAGA AGCATGCTGC AGTGCCTGAG 
AAGACCAAAG ATATTCCCAA GACTAAAGCT GATGCTCTGT CAAGTGATTT CGTGGCTCCG 
GCACATTCTG CTCCTAAGGT TTCTTCAGCT GCTCCTAAAA CCATCCCTCC AGGCCCACCA 
CAAACCCCAG ATGAGGATCC TTTCAGTGCT TTGGGCGACA CTCTTTCTGC CCCAGAACCA 
CCAAAAAAAC AACCTGACCT GAAACCTAAG GACATCGTAC ATGAGAAGGA TGTGACGTCA 
AAGAAAGGAG TTCGTGTTGG AGAAAGAGAC GACACACTCC CACCAGAATA CAGATTCAAA 
GAGGAAGATC TCAAGAAATA TCCTCCTCCT CAGAAAGATC CTTCCTTAGA TGCTAATGAA 
GCTCTGGATA TTCTGTCTGG GGATTTTACA ACTCCTTCCG CACCATCGGC TGTTTCCCCT 
GCCTCTAAAT CCTCTGCCAA GTCTTCAGAT TCAGCTTTAG ATGCTCTTGC TGGTGATTTC 
GTCGCTTCTT CTTCTGCGTC TTCAGTTCAT TCTGCCGTCT CTGGCCCCCC ACGCTCCGAC 
AGACTGTTGT CAGACGGAAC CTCATCAGCT CTAGATGCCC TGTCAGAAAC TCTGGGAGAC 
ATTAAAGCAG CCCCTGAACC CGCCCCTGTC CTACCTAAAA CTGTCGTTAA GGAGAAGACG 
GTCATAGAAG AGAGAGTGAG TAAACCGGGT GAGAGAGACG ACTCCCTTCC ACCAGATCAG 
CGGTTCACTG CGGAGGATCG CAAGGCATTT GAAGAAGCAA AGAAGAAAGA CGCCAAACCA 
AAACAGCCAT CAATGGACGA TTCAGCGGCT CTGGACATGT TGTCTAGTGA TTTTTCAGAT 
GTACCGACTG TAAAGCCCAC TGTATCTGAT GGCAAAAGCT TCACCCCTGA ACCAACACCA 
CCAACCTTTA AGGCAACCGG TCCGGTTTTA GACAAACTGG CAGACAACGT GCTTTCCAAC 
CTGACTGACC CCAAAGCCAA AGACAGCAAA GCAAAGGGAA AGGGGGCGAA ACCAAAGTCT 
AAACCAAAGA AACAGGCAGT GGAAGACTCT TCAGCCACAG ATCTGCTGTC CAGTAAACCG 
AGCTCAGATG TGGTGCCTTC AGCTTCCACA AAGGCTGGAA AAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161085.1
CDS	72..2579
Translation	MGQIFSWIRGTRDQPALQDIAVEQQSQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQVTDSKSTPASSAKVSAVSSGTGVAGTTGGAGVKPADPAKDTPQSTTIPSTKLGPAKVDPAAGKPSGSAGAQKQTSAQKVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_005161028.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161028.4

ORF Insert Sequence:

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGTCTCA GCAGGTCACA CCCACCACAA CATCTCAGGT CTCCACAGTG 
AAACCTGCAC AATATGAGAA AGGAGCTACA CAATCGTCTA CAGCAGCTGC GACTGTCAAA 
TCCGGCGCTG GAGATCCTGC AACCACCCAG AAAGGATCTT CTCAAGTCAA ACCTCAGGTC 
ACAGATTCTA AATCAACACC TGCATCTTCT GCTAAAGTCT CAGCTGTGAG CTCTGGGACT 
GGAGTTGCTG GTACAACCGG TGGAGCAGGA GTGAAACCAG CAGATCCTGC AAAAGATACG 
CCTCAGAGCA CCACCATTCC TTCAACCAAA CTGGGACCTG CTAAGGTGGA TCCGGCTGCT 
GGGAAGCCCT CTGGCTCAGC TGGTGCCCAA AAACAGACAA GTGCTCAAAA GGTGCAGGTT 
GAAGTGGGTC CTTCAGCAGC AAAAGTCAGT GCAGATGCTG ACGATCCGTT TGATGCCCTG 
TCTGGCACAT TACCATCATC ACAACCTTTG GCTCCTAAAG TCCACTACAC TGGACCTGAG 
ATCAAAGAGC CCAAACTGAA ATCAGAGAAA GGTGTTTTGT GTGGTGAGCG AGATGACACG 
CTGCCGCCCG GATACAGAAA GGAAGACCTG GAAAAGAAGC ATGGAGCTGG AGTTCCAGCT 
GGAGTTCCGG AGAAGCCTAA AGAAGTTCCC AAGCCCATAA GCACAGATGA TGCACTGGAA 
TCTCTCTCCT CTACGTTTGT GTCTCCAGCT CAAGCTGCTC CAAAGAAGAC AGACGTGAAA 
ACTGAAACAA TAGGAGCAGT GGATGTTAAA TCGGCAGGCA CCACTAACTT TGCTCCCCCT 
CCACCATCTC AGCAGAAACA ACCGGCGTCG TCTCAAGCTG CTGCTGTAAC CAAATCACCT 
GCTCCACCGG CAGACAAGAA AGCTAAAATG GAGACTCATG CACAACCAGC CAAAGCAAAG 
ACAGAGTCGG ACAGCTCCAT ATCGCTGGAC GCTCTCGATG CTTTGAGCGA CACACTGGGG 
GCTTCTAAAC CACCCCAAAA ATTACCAGAA CTCAAACCTG GACAGATAGT TGATGAGAAG 
AAACAGACCT CAGAGAAAGG TGTTCGTGTT GGAGAAAGAG ACGACACACT TCCACCACAA 
TACAGATTCA AAGAGGAAGA TCTCAAGAAA TATCCTCCAC CTGCGAAAGA GCCTTCACTG 
AACACAGATG ATGCGATGGA CATACTTTCA GGAGATTTCA CGACCCCTGC ATCGGCACCT 
GTTGTCAAAG CTCCTGTTCC TCCTGCACAG GACACAAAGA AGCATGCTGC AGTGCCTGAG 
AAGACCAAAG ATATTCCCAA GACTAAAGCT GATGCTCTGT CAAGTGATTT CGTGGCTCCG 
GCACATTCTG CTCCTAAGGT TTCTTCAGCT GCTCCTAAAA CCATCCCTCC AGGCCCACCA 
CAAACCCCAG ATGAGGATCC TTTCAGTGCT TTGGGCGACA CTCTTTCTGC CCCAGAACCA 
CCAAAAAAAC AACCTGACCT GAAACCTAAG GACATCGTAC ATGAGAAGGA TGTGACGTCA 
AAGAAAGGAG TTCGTGTTGG AGAAAGAGAC GACACACTCC CACCAGAATA CAGATTCAAA 
GAGGAAGATC TCAAGAAATA TCCTCCTCCT CAGAAAGATC CTTCCTTAGA TGCTAATGAA 
GCTCTGGATA TTCTGTCTGG GGATTTTACA ACTCCTTCCG CACCATCGGC TGTTTCCCCT 
GCCTCTAAAT CCTCTGCCAA GTCTTCAGAT TCAGCTTTAG ATGCTCTTGC TGGTGATTTC 
GTCGCTTCTT CTTCTGCGTC TTCAGTTCAT TCTGCCGTCT CTGGCCCCCC ACGCTCCGAC 
AGACTGTTGT CAGACGGAAC CTCATCAGCT CTAGATGCCC TGTCAGAAAC TCTGGGAGAC 
ATTAAAGCAG CCCCTGAACC CGCCCCTGTC CTACCTAAAA CTGTCGTTAA GGAGAAGACG 
GTCATAGAAG AGAGAGTGAG TAAACCGGGT GAGAGAGACG ACTCCCTTCC ACCAGATCAG 
CGGTTCACTG CGGAGGATCG CAAGGCATTT GAAGAAGCAA AGAAGAAAGA CGCCAAACCA 
AAACAGCCAT CAATGGACGA TTCAGCGGCT CTGGACATGT TGTCTAGTGA TTTTTCAGAT 
GTACCGACTG TAAAGCCCAC TGTATCTGAT GGCAAAAGCT TCACCCCTGA ACCAACACCA 
CCAACCTTTA AGGCAACCGG TCCGGTTTTA GACAAACTGG CAGACAACGT GCTTTCCAAC 
CTGACTGACC CCAAAGCCAA AGACAGCAAA GCAAAGGGAA AGGGGGCGAA ACCAAAGTCT 
AAACCAAAGA AACAGGCAGT GGAAGACTCT TCAGCCACAG ATCTGCTGTC CAGTAAACCG 
AGCTCAGATG TGGTGCCTTC AGCTTCCACA AAGGCTGGAA AAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_005161028.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161085.1 Danio rerio calpastatin (cast), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa25606
Clone ID Related Accession (Same CDS sequence)	XM_005161028.4 , XM_005161028.3
Accession Version	XM_005161028.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	calpastatin isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005161028.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGTCTCA GCAGGTCACA CCCACCACAA CATCTCAGGT CTCCACAGTG 
AAACCTGCAC AATATGAGAA AGGAGCTACA CAATCGTCTA CAGCAGCTGC GACTGTCAAA 
TCCGGCGCTG GAGATCCTGC AACCACCCAG AAAGGATCTT CTCAAGTCAA ACCTCAGGTC 
ACAGATTCTA AATCAACACC TGCATCTTCT GCTAAAGTCT CAGCTGTGAG CTCTGGGACT 
GGAGTTGCTG GTACAACCGG TGGAGCAGGA GTGAAACCAG CAGATCCTGC AAAAGATACG 
CCTCAGAGCA CCACCATTCC TTCAACCAAA CTGGGACCTG CTAAGGTGGA TCCGGCTGCT 
GGGAAGCCCT CTGGCTCAGC TGGTGCCCAA AAACAGACAA GTGCTCAAAA GGTGCAGGTT 
GAAGTGGGTC CTTCAGCAGC AAAAGTCAGT GCAGATGCTG ACGATCCGTT TGATGCCCTG 
TCTGGCACAT TACCATCATC ACAACCTTTG GCTCCTAAAG TCCACTACAC TGGACCTGAG 
ATCAAAGAGC CCAAACTGAA ATCAGAGAAA GGTGTTTTGT GTGGTGAGCG AGATGACACG 
CTGCCGCCCG GATACAGAAA GGAAGACCTG GAAAAGAAGC ATGGAGCTGG AGTTCCAGCT 
GGAGTTCCGG AGAAGCCTAA AGAAGTTCCC AAGCCCATAA GCACAGATGA TGCACTGGAA 
TCTCTCTCCT CTACGTTTGT GTCTCCAGCT CAAGCTGCTC CAAAGAAGAC AGACGTGAAA 
ACTGAAACAA TAGGAGCAGT GGATGTTAAA TCGGCAGGCA CCACTAACTT TGCTCCCCCT 
CCACCATCTC AGCAGAAACA ACCGGCGTCG TCTCAAGCTG CTGCTGTAAC CAAATCACCT 
GCTCCACCGG CAGACAAGAA AGCTAAAATG GAGACTCATG CACAACCAGC CAAAGCAAAG 
ACAGAGTCGG ACAGCTCCAT ATCGCTGGAC GCTCTCGATG CTTTGAGCGA CACACTGGGG 
GCTTCTAAAC CACCCCAAAA ATTACCAGAA CTCAAACCTG GACAGATAGT TGATGAGAAG 
AAACAGACCT CAGAGAAAGG TGTTCGTGTT GGAGAAAGAG ACGACACACT TCCACCACAA 
TACAGATTCA AAGAGGAAGA TCTCAAGAAA TATCCTCCAC CTGCGAAAGA GCCTTCACTG 
AACACAGATG ATGCGATGGA CATACTTTCA GGAGATTTCA CGACCCCTGC ATCGGCACCT 
GTTGTCAAAG CTCCTGTTCC TCCTGCACAG GACACAAAGA AGCATGCTGC AGTGCCTGAG 
AAGACCAAAG ATATTCCCAA GACTAAAGCT GATGCTCTGT CAAGTGATTT CGTGGCTCCG 
GCACATTCTG CTCCTAAGGT TTCTTCAGCT GCTCCTAAAA CCATCCCTCC AGGCCCACCA 
CAAACCCCAG ATGAGGATCC TTTCAGTGCT TTGGGCGACA CTCTTTCTGC CCCAGAACCA 
CCAAAAAAAC AACCTGACCT GAAACCTAAG GACATCGTAC ATGAGAAGGA TGTGACGTCA 
AAGAAAGGAG TTCGTGTTGG AGAAAGAGAC GACACACTCC CACCAGAATA CAGATTCAAA 
GAGGAAGATC TCAAGAAATA TCCTCCTCCT CAGAAAGATC CTTCCTTAGA TGCTAATGAA 
GCTCTGGATA TTCTGTCTGG GGATTTTACA ACTCCTTCCG CACCATCGGC TGTTTCCCCT 
GCCTCTAAAT CCTCTGCCAA GTCTTCAGAT TCAGCTTTAG ATGCTCTTGC TGGTGATTTC 
GTCGCTTCTT CTTCTGCGTC TTCAGTTCAT TCTGCCGTCT CTGGCCCCCC ACGCTCCGAC 
AGACTGTTGT CAGACGGAAC CTCATCAGCT CTAGATGCCC TGTCAGAAAC TCTGGGAGAC 
ATTAAAGCAG CCCCTGAACC CGCCCCTGTC CTACCTAAAA CTGTCGTTAA GGAGAAGACG 
GTCATAGAAG AGAGAGTGAG TAAACCGGGT GAGAGAGACG ACTCCCTTCC ACCAGATCAG 
CGGTTCACTG CGGAGGATCG CAAGGCATTT GAAGAAGCAA AGAAGAAAGA CGCCAAACCA 
AAACAGCCAT CAATGGACGA TTCAGCGGCT CTGGACATGT TGTCTAGTGA TTTTTCAGAT 
GTACCGACTG TAAAGCCCAC TGTATCTGAT GGCAAAAGCT TCACCCCTGA ACCAACACCA 
CCAACCTTTA AGGCAACCGG TCCGGTTTTA GACAAACTGG CAGACAACGT GCTTTCCAAC 
CTGACTGACC CCAAAGCCAA AGACAGCAAA GCAAAGGGAA AGGGGGCGAA ACCAAAGTCT 
AAACCAAAGA AACAGGCAGT GGAAGACTCT TCAGCCACAG ATCTGCTGTC CAGTAAACCG 
AGCTCAGATG TGGTGCCTTC AGCTTCCACA AAGGCTGGAA AAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161085.1
CDS	68..2575
Translation	MGQIFSWIRGTRDQPALQDIAVEQQSQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQVTDSKSTPASSAKVSAVSSGTGVAGTTGGAGVKPADPAKDTPQSTTIPSTKLGPAKVDPAAGKPSGSAGAQKQTSAQKVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_005161028.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161028.3

ORF Insert Sequence:

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGTCTCA GCAGGTCACA CCCACCACAA CATCTCAGGT CTCCACAGTG 
AAACCTGCAC AATATGAGAA AGGAGCTACA CAATCGTCTA CAGCAGCTGC GACTGTCAAA 
TCCGGCGCTG GAGATCCTGC AACCACCCAG AAAGGATCTT CTCAAGTCAA ACCTCAGGTC 
ACAGATTCTA AATCAACACC TGCATCTTCT GCTAAAGTCT CAGCTGTGAG CTCTGGGACT 
GGAGTTGCTG GTACAACCGG TGGAGCAGGA GTGAAACCAG CAGATCCTGC AAAAGATACG 
CCTCAGAGCA CCACCATTCC TTCAACCAAA CTGGGACCTG CTAAGGTGGA TCCGGCTGCT 
GGGAAGCCCT CTGGCTCAGC TGGTGCCCAA AAACAGACAA GTGCTCAAAA GGTGCAGGTT 
GAAGTGGGTC CTTCAGCAGC AAAAGTCAGT GCAGATGCTG ACGATCCGTT TGATGCCCTG 
TCTGGCACAT TACCATCATC ACAACCTTTG GCTCCTAAAG TCCACTACAC TGGACCTGAG 
ATCAAAGAGC CCAAACTGAA ATCAGAGAAA GGTGTTTTGT GTGGTGAGCG AGATGACACG 
CTGCCGCCCG GATACAGAAA GGAAGACCTG GAAAAGAAGC ATGGAGCTGG AGTTCCAGCT 
GGAGTTCCGG AGAAGCCTAA AGAAGTTCCC AAGCCCATAA GCACAGATGA TGCACTGGAA 
TCTCTCTCCT CTACGTTTGT GTCTCCAGCT CAAGCTGCTC CAAAGAAGAC AGACGTGAAA 
ACTGAAACAA TAGGAGCAGT GGATGTTAAA TCGGCAGGCA CCACTAACTT TGCTCCCCCT 
CCACCATCTC AGCAGAAACA ACCGGCGTCG TCTCAAGCTG CTGCTGTAAC CAAATCACCT 
GCTCCACCGG CAGACAAGAA AGCTAAAATG GAGACTCATG CACAACCAGC CAAAGCAAAG 
ACAGAGTCGG ACAGCTCCAT ATCGCTGGAC GCTCTCGATG CTTTGAGCGA CACACTGGGG 
GCTTCTAAAC CACCCCAAAA ATTACCAGAA CTCAAACCTG GACAGATAGT TGATGAGAAG 
AAACAGACCT CAGAGAAAGG TGTTCGTGTT GGAGAAAGAG ACGACACACT TCCACCACAA 
TACAGATTCA AAGAGGAAGA TCTCAAGAAA TATCCTCCAC CTGCGAAAGA GCCTTCACTG 
AACACAGATG ATGCGATGGA CATACTTTCA GGAGATTTCA CGACCCCTGC ATCGGCACCT 
GTTGTCAAAG CTCCTGTTCC TCCTGCACAG GACACAAAGA AGCATGCTGC AGTGCCTGAG 
AAGACCAAAG ATATTCCCAA GACTAAAGCT GATGCTCTGT CAAGTGATTT CGTGGCTCCG 
GCACATTCTG CTCCTAAGGT TTCTTCAGCT GCTCCTAAAA CCATCCCTCC AGGCCCACCA 
CAAACCCCAG ATGAGGATCC TTTCAGTGCT TTGGGCGACA CTCTTTCTGC CCCAGAACCA 
CCAAAAAAAC AACCTGACCT GAAACCTAAG GACATCGTAC ATGAGAAGGA TGTGACGTCA 
AAGAAAGGAG TTCGTGTTGG AGAAAGAGAC GACACACTCC CACCAGAATA CAGATTCAAA 
GAGGAAGATC TCAAGAAATA TCCTCCTCCT CAGAAAGATC CTTCCTTAGA TGCTAATGAA 
GCTCTGGATA TTCTGTCTGG GGATTTTACA ACTCCTTCCG CACCATCGGC TGTTTCCCCT 
GCCTCTAAAT CCTCTGCCAA GTCTTCAGAT TCAGCTTTAG ATGCTCTTGC TGGTGATTTC 
GTCGCTTCTT CTTCTGCGTC TTCAGTTCAT TCTGCCGTCT CTGGCCCCCC ACGCTCCGAC 
AGACTGTTGT CAGACGGAAC CTCATCAGCT CTAGATGCCC TGTCAGAAAC TCTGGGAGAC 
ATTAAAGCAG CCCCTGAACC CGCCCCTGTC CTACCTAAAA CTGTCGTTAA GGAGAAGACG 
GTCATAGAAG AGAGAGTGAG TAAACCGGGT GAGAGAGACG ACTCCCTTCC ACCAGATCAG 
CGGTTCACTG CGGAGGATCG CAAGGCATTT GAAGAAGCAA AGAAGAAAGA CGCCAAACCA 
AAACAGCCAT CAATGGACGA TTCAGCGGCT CTGGACATGT TGTCTAGTGA TTTTTCAGAT 
GTACCGACTG TAAAGCCCAC TGTATCTGAT GGCAAAAGCT TCACCCCTGA ACCAACACCA 
CCAACCTTTA AGGCAACCGG TCCGGTTTTA GACAAACTGG CAGACAACGT GCTTTCCAAC 
CTGACTGACC CCAAAGCCAA AGACAGCAAA GCAAAGGGAA AGGGGGCGAA ACCAAAGTCT 
AAACCAAAGA AACAGGCAGT GGAAGACTCT TCAGCCACAG ATCTGCTGTC CAGTAAACCG 
AGCTCAGATG TGGTGCCTTC AGCTTCCACA AAGGCTGGAA AAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_005161029.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161086.1 Danio rerio calpastatin (cast), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ODa25607
Clone ID Related Accession (Same CDS sequence)	XM_005161029.3
Accession Version	XM_005161029.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	calpastatin isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005161029.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCACA AAAAGAGATG TCATGGTCGT AAGAAAAACA AAAAGGAGAA TGGAGACACT 
TCATTTCAAA AACAAGAATA CGCTCCACAG ACATCAAGGT TCATGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGAA AGGAGCTACA 
CAATCGTCTA CAGCAGCTGC GACTGTCAAA TCCGGCGCTG GAGATCCTGC AACCACCCAG 
AAAGGATCTT CTCAAGTCAA ACCTCAGGTC ACAGATTCTA AATCAACACC TGCATCTTCT 
GCTAAAGTCT CAGCTGTGAG CTCTGGGACT GGAGTTGCTG GTACAACCGG TGGAGCAGGA 
GTGAAACCAG CAGATCCTGC AAAAGATACG CCTCAGAGCA CCACCATTCC TTCAACCAAA 
CTGGGACCTG CTAAGGTGGA TCCGGCTGCT GGGAAGCCCT CTGGCTCAGC TGGTGCCCAA 
AAACAGACAA GTGCTCAAAA GGTGCAGGTT GAAGTGGGTC CTTCAGCAGC AAAAGTCAGT 
GCAGATGCTG ACGATCCGTT TGATGCCCTG TCTGGCACAT TACCATCATC ACAACCTTTG 
GCTCCTAAAG TCCACTACAC TGGACCTGAG ATCAAAGAGC CCAAACTGAA ATCAGAGAAA 
GGTGTTTTGT GTGGTGAGCG AGATGACACG CTGCCGCCCG GATACAGAAA GGAAGACCTG 
GAAAAGAAGC ATGGAGCTGG AGTTCCAGCT GGAGTTCCGG AGAAGCCTAA AGAAGTTCCC 
AAGCCCATAA GCACAGATGA TGCACTGGAA TCTCTCTCCT CTACGTTTGT GTCTCCAGCT 
CAAGCTGCTC CAAAGAAGAC AGACGTGAAA ACTGAAACAA TAGGAGCAGT GGATGTTAAA 
TCGGCAGGCA CCACTAACTT TGCTCCCCCT CCACCATCTC AGCAGAAACA ACCGGCGTCG 
TCTCAAGCTG CTGCTGTAAC CAAATCACCT GCTCCACCGG CAGACAAGAA AGCTAAAATG 
GAGACTCATG CACAACCAGC CAAAGCAAAG ACAGAGTCGG ACAGCTCCAT ATCGCTGGAC 
GCTCTCGATG CTTTGAGCGA CACACTGGGG GCTTCTAAAC CACCCCAAAA ATTACCAGAA 
CTCAAACCTG GACAGATAGT TGATGAGAAG AAACAGACCT CAGAGAAAGG TGTTCGTGTT 
GGAGAAAGAG ACGACACACT TCCACCACAA TACAGATTCA AAGAGGAAGA TCTCAAGAAA 
TATCCTCCAC CTGCGAAAGA GCCTTCACTG AACACAGATG ATGCGATGGA CATACTTTCA 
GGAGATTTCA CGACCCCTGC ATCGGCACCT GTTGTCAAAG CTCCTGTTCC TCCTGCACAG 
GACACAAAGA AGCATGCTGC AGTGCCTGAG AAGACCAAAG ATATTCCCAA GACTAAAGCT 
GATGCTCTGT CAAGTGATTT CGTGGCTCCG GCACATTCTG CTCCTAAGGT TTCTTCAGCT 
GCTCCTAAAA CCATCCCTCC AGGCCCACCA CAAACCCCAG ATGAGGATCC TTTCAGTGCT 
TTGGGCGACA CTCTTTCTGC CCCAGAACCA CCAAAAAAAC AACCTGACCT GAAACCTAAG 
GACATCGTAC ATGAGAAGGA TGTGACGTCA AAGAAAGGAG TTCGTGTTGG AGAAAGAGAC 
GACACACTCC CACCAGAATA CAGATTCAAA GAGGAAGATC TCAAGAAATA TCCTCCTCCT 
CAGAAAGATC CTTCCTTAGA TGCTAATGAA GCTCTGGATA TTCTGTCTGG GGATTTTACA 
ACTCCTTCCG CACCATCGGC TGTTTCCCCT GCCTCTAAAT CCTCTGCCAA GTCTTCAGAT 
TCAGCTTTAG ATGCTCTTGC TGGTGATTTC GTCGCTTCTT CTTCTGCGTC TTCAGTTCAT 
TCTGCCGTCT CTGGCCCCCC ACGCTCCGAC AGACTGTTGT CAGACGGAAC CTCATCAGCT 
CTAGATGCCC TGTCAGAAAC TCTGGGAGAC ATTAAAGCAG CCCCTGAACC CGCCCCTGTC 
CTACCTAAAA CTGTCGTTAA GGAGAAGACG GTCATAGAAG AGAGAGTGAG TAAACCGGGT 
GAGAGAGACG ACTCCCTTCC ACCAGATCAG CGGTTCACTG CGGAGGATCG CAAGGCATTT 
GAAGAAGCAA AGAAGAAAGA CGCCAAACCA AAACAGCCAT CAATGGACGA TTCAGCGGCT 
CTGGACATGT TGTCTAGTGA TTTTTCAGAT GTACCGACTG TAAAGCCCAC TGTATCTGAT 
GGCAAAAGCT TCACCCCTGA ACCAACACCA CCAACCTTTA AGGCAACCGG TCCGGTTTTA 
GACAAACTGG CAGACAACGT GCTTTCCAAC CTGACTGACC CCAAAGCCAA AGACAGCAAA 
GCAAAGAAAC AGGCAGTGGA AGACTCTTCA GCCACAGATC TGCTGTCCAG TAAACCGAGC 
TCAGATGTGG TGCCTTCAGC TTCCACAAAG GCTGGAAAAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161086.1
CDS	43..2547
Translation	MPHKKRCHGRKKNKKENGDTSFQKQEYAPQTSRFMSQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQVTDSKSTPASSAKVSAVSSGTGVAGTTGGAGVKPADPAKDTPQSTTIPSTKLGPAKVDPAAGKPSGSAGAQKQTSAQKVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_005161029.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161029.3

ORF Insert Sequence:

ATGCCGCACA AAAAGAGATG TCATGGTCGT AAGAAAAACA AAAAGGAGAA TGGAGACACT 
TCATTTCAAA AACAAGAATA CGCTCCACAG ACATCAAGGT TCATGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGAA AGGAGCTACA 
CAATCGTCTA CAGCAGCTGC GACTGTCAAA TCCGGCGCTG GAGATCCTGC AACCACCCAG 
AAAGGATCTT CTCAAGTCAA ACCTCAGGTC ACAGATTCTA AATCAACACC TGCATCTTCT 
GCTAAAGTCT CAGCTGTGAG CTCTGGGACT GGAGTTGCTG GTACAACCGG TGGAGCAGGA 
GTGAAACCAG CAGATCCTGC AAAAGATACG CCTCAGAGCA CCACCATTCC TTCAACCAAA 
CTGGGACCTG CTAAGGTGGA TCCGGCTGCT GGGAAGCCCT CTGGCTCAGC TGGTGCCCAA 
AAACAGACAA GTGCTCAAAA GGTGCAGGTT GAAGTGGGTC CTTCAGCAGC AAAAGTCAGT 
GCAGATGCTG ACGATCCGTT TGATGCCCTG TCTGGCACAT TACCATCATC ACAACCTTTG 
GCTCCTAAAG TCCACTACAC TGGACCTGAG ATCAAAGAGC CCAAACTGAA ATCAGAGAAA 
GGTGTTTTGT GTGGTGAGCG AGATGACACG CTGCCGCCCG GATACAGAAA GGAAGACCTG 
GAAAAGAAGC ATGGAGCTGG AGTTCCAGCT GGAGTTCCGG AGAAGCCTAA AGAAGTTCCC 
AAGCCCATAA GCACAGATGA TGCACTGGAA TCTCTCTCCT CTACGTTTGT GTCTCCAGCT 
CAAGCTGCTC CAAAGAAGAC AGACGTGAAA ACTGAAACAA TAGGAGCAGT GGATGTTAAA 
TCGGCAGGCA CCACTAACTT TGCTCCCCCT CCACCATCTC AGCAGAAACA ACCGGCGTCG 
TCTCAAGCTG CTGCTGTAAC CAAATCACCT GCTCCACCGG CAGACAAGAA AGCTAAAATG 
GAGACTCATG CACAACCAGC CAAAGCAAAG ACAGAGTCGG ACAGCTCCAT ATCGCTGGAC 
GCTCTCGATG CTTTGAGCGA CACACTGGGG GCTTCTAAAC CACCCCAAAA ATTACCAGAA 
CTCAAACCTG GACAGATAGT TGATGAGAAG AAACAGACCT CAGAGAAAGG TGTTCGTGTT 
GGAGAAAGAG ACGACACACT TCCACCACAA TACAGATTCA AAGAGGAAGA TCTCAAGAAA 
TATCCTCCAC CTGCGAAAGA GCCTTCACTG AACACAGATG ATGCGATGGA CATACTTTCA 
GGAGATTTCA CGACCCCTGC ATCGGCACCT GTTGTCAAAG CTCCTGTTCC TCCTGCACAG 
GACACAAAGA AGCATGCTGC AGTGCCTGAG AAGACCAAAG ATATTCCCAA GACTAAAGCT 
GATGCTCTGT CAAGTGATTT CGTGGCTCCG GCACATTCTG CTCCTAAGGT TTCTTCAGCT 
GCTCCTAAAA CCATCCCTCC AGGCCCACCA CAAACCCCAG ATGAGGATCC TTTCAGTGCT 
TTGGGCGACA CTCTTTCTGC CCCAGAACCA CCAAAAAAAC AACCTGACCT GAAACCTAAG 
GACATCGTAC ATGAGAAGGA TGTGACGTCA AAGAAAGGAG TTCGTGTTGG AGAAAGAGAC 
GACACACTCC CACCAGAATA CAGATTCAAA GAGGAAGATC TCAAGAAATA TCCTCCTCCT 
CAGAAAGATC CTTCCTTAGA TGCTAATGAA GCTCTGGATA TTCTGTCTGG GGATTTTACA 
ACTCCTTCCG CACCATCGGC TGTTTCCCCT GCCTCTAAAT CCTCTGCCAA GTCTTCAGAT 
TCAGCTTTAG ATGCTCTTGC TGGTGATTTC GTCGCTTCTT CTTCTGCGTC TTCAGTTCAT 
TCTGCCGTCT CTGGCCCCCC ACGCTCCGAC AGACTGTTGT CAGACGGAAC CTCATCAGCT 
CTAGATGCCC TGTCAGAAAC TCTGGGAGAC ATTAAAGCAG CCCCTGAACC CGCCCCTGTC 
CTACCTAAAA CTGTCGTTAA GGAGAAGACG GTCATAGAAG AGAGAGTGAG TAAACCGGGT 
GAGAGAGACG ACTCCCTTCC ACCAGATCAG CGGTTCACTG CGGAGGATCG CAAGGCATTT 
GAAGAAGCAA AGAAGAAAGA CGCCAAACCA AAACAGCCAT CAATGGACGA TTCAGCGGCT 
CTGGACATGT TGTCTAGTGA TTTTTCAGAT GTACCGACTG TAAAGCCCAC TGTATCTGAT 
GGCAAAAGCT TCACCCCTGA ACCAACACCA CCAACCTTTA AGGCAACCGG TCCGGTTTTA 
GACAAACTGG CAGACAACGT GCTTTCCAAC CTGACTGACC CCAAAGCCAA AGACAGCAAA 
GCAAAGAAAC AGGCAGTGGA AGACTCTTCA GCCACAGATC TGCTGTCCAG TAAACCGAGC 
TCAGATGTGG TGCCTTCAGC TTCCACAAAG GCTGGAAAAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_005161031.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161088.1 Danio rerio calpastatin (cast), transcript variant X9, mRNA.

Price & Availability↑

CloneID	ODa25609
Clone ID Related Accession (Same CDS sequence)	XM_005161031.4 , XM_005161031.3
Accession Version	XM_005161031.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calpastatin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005161031.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTACG CAATGTATTG GGCGCGCAAC AATGGAGACA CTTCATTTCA AAAACAAGAA 
TACGCTCCAC AGACATCAAG GTTCATGTCT CAGCAGGTCA CACCCACCAC AACATCTCAG 
GTCTCCACAG TGAAACCTGC ACAATATGAG AAAGGAGCTA CACAATCGTC TACAGCAGCT 
GCGACTGTCA AATCCGGCGC TGGAGATCCT GCAACCACCC AGAAAGGATC TTCTCAAGTC 
AAACCTCAGG TCACAGATTC TAAATCAACA CCTGCATCTT CTGCTAAAGT CTCAGCTGTG 
AGCTCTGGGA CTGGAGTTGC TGGTACAACC GGTGGAGCAG GAGTGAAACC AGCAGATCCT 
GCAAAAGATA CGCCTCAGAG CACCACCATT CCTTCAACCA AACTGGGACC TGCTAAGGTG 
GATCCGGCTG CTGGGAAGCC CTCTGGCTCA GCTGGTGCCC AAAAACAGAC AAGTGCTCAA 
AAGGTGCAGG TTGAAGTGGG TCCTTCAGCA GCAAAAGCTG ACGATCCGTT TGATGCCCTG 
TCTGGCACAT TACCATCATC ACAACCTTTG GCTCCTAAAG TCCACTACAC TGGACCTGAG 
ATCAAAGAGC CCAAACTGAA ATCAGAGAAA GGTGTTTTGT GTGGTGAGCG AGATGACACG 
CTGCCGCCCG GATACAGAAA GGAAGACCTG GAAAAGAAGC ATGGAGCTGG AGTTCCAGCT 
GGAGTTCCGG AGAAGCCTAA AGAAGTTCCC AAGCCCATAA GCACAGATGA TGCACTGGAA 
TCTCTCTCCT CTACGTTTGT GTCTCCAGCT CAAGCTGCTC CAAAGAAGAC AGACGTGAAA 
ACTGAAACAA TAGGAGCAGT GGATGTTAAA TCGGCAGGCA CCACTAACTT TGCTCCCCCT 
CCACCATCTC AGCAGAAACA ACCGGCGTCG TCTCAAGCTG CTGCTGTAAC CAAATCACCT 
GCTCCACCGG CAGACAAGAA AGCTAAAATG GAGACTCATG CACAACCAGC CAAAGCAAAG 
ACAGAGTCGG ACAGCTCCAT ATCGCTGGAC GCTCTCGATG CTTTGAGCGA CACACTGGGG 
GCTTCTAAAC CACCCCAAAA ATTACCAGAA CTCAAACCTG GACAGATAGT TGATGAGAAG 
AAACAGACCT CAGAGAAAGG TGTTCGTGTT GGAGAAAGAG ACGACACACT TCCACCACAA 
TACAGATTCA AAGAGGAAGA TCTCAAGAAA TATCCTCCAC CTGCGAAAGA GCCTTCACTG 
AACACAGATG ATGCGATGGA CATACTTTCA GGAGATTTCA CGACCCCTGC ATCGGCACCT 
GTTGTCAAAG CTCCTGTTCC TCCTGCACAG GACACAAAGA AGCATGCTGC AGTGCCTGAG 
AAGACCAAAG ATATTCCCAA GACTAAAGCT GATGCTCTGT CAAGTGATTT CGTGGCTCCG 
GCACATTCTG CTCCTAAGGT TTCTTCAGCT GCTCCTAAAA CCATCCCTCC AGGCCCACCA 
CAAACCCCAG ATGAGGATCC TTTCAGTGCT TTGGGCGACA CTCTTTCTGC CCCAGAACCA 
CCAAAAAAAC AACCTGACCT GAAACCTAAG GACATCGTAC ATGAGAAGGA TGTGACGTCA 
AAGAAAGGAG TTCGTGTTGG AGAAAGAGAC GACACACTCC CACCAGAATA CAGATTCAAA 
GAGGAAGATC TCAAGAAATA TCCTCCTCCT CAGAAAGATC CTTCCTTAGA TGCTAATGAA 
GCTCTGGATA TTCTGTCTGG GGATTTTACA ACTCCTTCCG CACCATCGGC TGTTTCCCCT 
GCCTCTAAAT CCTCTGCCAA GTCTTCAGAT TCAGCTTTAG ATGCTCTTGC TGGTGATTTC 
GTCGCTTCTT CTTCTGCGTC TTCAGTTCAT TCTGCCGTCT CTGGCCCCCC ACGCTCCGAC 
AGACTGTTGT CAGACGGAAC CTCATCAGCT CTAGATGCCC TGTCAGAAAC TCTGGGAGAC 
ATTAAAGCAG CCCCTGAACC CGCCCCTGTC CTACCTAAAA CTGTCGTTAA GGAGAAGACG 
GTCATAGAAG AGAGAGTGAG TAAACCGGGT GAGAGAGACG ACTCCCTTCC ACCAGATCAG 
CGGTTCACTG CGGAGGATCG CAAGGCATTT GAAGAAGCAA AGAAGAAAGA CGCCAAACCA 
AAACAGCCAT CAATGGACGA TTCAGCGGCT CTGGACATGT TGTCTAGTGA TTTTTCAGAT 
GTACCGACTG TAAAGCCCAC TGTATCTGAT GGCAAAAGCT TCACCCCTGA ACCAACACCA 
CCAACCTTTA AGGCAACCGG TCCGGTTTTA GACAAACTGG CAGACAACGT GCTTTCCAAC 
CTGACTGACC CCAAAGCCAA AGACAGCAAA GCAAAGAAAC AGGCAGTGGA AGACTCTTCA 
GCCACAGATC TGCTGTCCAG TAAACCGAGC TCAGATGTGG TGCCTTCAGC TTCCACAAAG 
GCTGGAAAAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161088.1
CDS	112..2586
Translation	MAYAMYWARNNGDTSFQKQEYAPQTSRFMSQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQVTDSKSTPASSAKVSAVSSGTGVAGTTGGAGVKPADPAKDTPQSTTIPSTKLGPAKVDPAAGKPSGSAGAQKQTSAQKVQVEVGPSAAKADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_005161031.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161031.4

ORF Insert Sequence:

ATGGCGTACG CAATGTATTG GGCGCGCAAC AATGGAGACA CTTCATTTCA AAAACAAGAA 
TACGCTCCAC AGACATCAAG GTTCATGTCT CAGCAGGTCA CACCCACCAC AACATCTCAG 
GTCTCCACAG TGAAACCTGC ACAATATGAG AAAGGAGCTA CACAATCGTC TACAGCAGCT 
GCGACTGTCA AATCCGGCGC TGGAGATCCT GCAACCACCC AGAAAGGATC TTCTCAAGTC 
AAACCTCAGG TCACAGATTC TAAATCAACA CCTGCATCTT CTGCTAAAGT CTCAGCTGTG 
AGCTCTGGGA CTGGAGTTGC TGGTACAACC GGTGGAGCAG GAGTGAAACC AGCAGATCCT 
GCAAAAGATA CGCCTCAGAG CACCACCATT CCTTCAACCA AACTGGGACC TGCTAAGGTG 
GATCCGGCTG CTGGGAAGCC CTCTGGCTCA GCTGGTGCCC AAAAACAGAC AAGTGCTCAA 
AAGGTGCAGG TTGAAGTGGG TCCTTCAGCA GCAAAAGCTG ACGATCCGTT TGATGCCCTG 
TCTGGCACAT TACCATCATC ACAACCTTTG GCTCCTAAAG TCCACTACAC TGGACCTGAG 
ATCAAAGAGC CCAAACTGAA ATCAGAGAAA GGTGTTTTGT GTGGTGAGCG AGATGACACG 
CTGCCGCCCG GATACAGAAA GGAAGACCTG GAAAAGAAGC ATGGAGCTGG AGTTCCAGCT 
GGAGTTCCGG AGAAGCCTAA AGAAGTTCCC AAGCCCATAA GCACAGATGA TGCACTGGAA 
TCTCTCTCCT CTACGTTTGT GTCTCCAGCT CAAGCTGCTC CAAAGAAGAC AGACGTGAAA 
ACTGAAACAA TAGGAGCAGT GGATGTTAAA TCGGCAGGCA CCACTAACTT TGCTCCCCCT 
CCACCATCTC AGCAGAAACA ACCGGCGTCG TCTCAAGCTG CTGCTGTAAC CAAATCACCT 
GCTCCACCGG CAGACAAGAA AGCTAAAATG GAGACTCATG CACAACCAGC CAAAGCAAAG 
ACAGAGTCGG ACAGCTCCAT ATCGCTGGAC GCTCTCGATG CTTTGAGCGA CACACTGGGG 
GCTTCTAAAC CACCCCAAAA ATTACCAGAA CTCAAACCTG GACAGATAGT TGATGAGAAG 
AAACAGACCT CAGAGAAAGG TGTTCGTGTT GGAGAAAGAG ACGACACACT TCCACCACAA 
TACAGATTCA AAGAGGAAGA TCTCAAGAAA TATCCTCCAC CTGCGAAAGA GCCTTCACTG 
AACACAGATG ATGCGATGGA CATACTTTCA GGAGATTTCA CGACCCCTGC ATCGGCACCT 
GTTGTCAAAG CTCCTGTTCC TCCTGCACAG GACACAAAGA AGCATGCTGC AGTGCCTGAG 
AAGACCAAAG ATATTCCCAA GACTAAAGCT GATGCTCTGT CAAGTGATTT CGTGGCTCCG 
GCACATTCTG CTCCTAAGGT TTCTTCAGCT GCTCCTAAAA CCATCCCTCC AGGCCCACCA 
CAAACCCCAG ATGAGGATCC TTTCAGTGCT TTGGGCGACA CTCTTTCTGC CCCAGAACCA 
CCAAAAAAAC AACCTGACCT GAAACCTAAG GACATCGTAC ATGAGAAGGA TGTGACGTCA 
AAGAAAGGAG TTCGTGTTGG AGAAAGAGAC GACACACTCC CACCAGAATA CAGATTCAAA 
GAGGAAGATC TCAAGAAATA TCCTCCTCCT CAGAAAGATC CTTCCTTAGA TGCTAATGAA 
GCTCTGGATA TTCTGTCTGG GGATTTTACA ACTCCTTCCG CACCATCGGC TGTTTCCCCT 
GCCTCTAAAT CCTCTGCCAA GTCTTCAGAT TCAGCTTTAG ATGCTCTTGC TGGTGATTTC 
GTCGCTTCTT CTTCTGCGTC TTCAGTTCAT TCTGCCGTCT CTGGCCCCCC ACGCTCCGAC 
AGACTGTTGT CAGACGGAAC CTCATCAGCT CTAGATGCCC TGTCAGAAAC TCTGGGAGAC 
ATTAAAGCAG CCCCTGAACC CGCCCCTGTC CTACCTAAAA CTGTCGTTAA GGAGAAGACG 
GTCATAGAAG AGAGAGTGAG TAAACCGGGT GAGAGAGACG ACTCCCTTCC ACCAGATCAG 
CGGTTCACTG CGGAGGATCG CAAGGCATTT GAAGAAGCAA AGAAGAAAGA CGCCAAACCA 
AAACAGCCAT CAATGGACGA TTCAGCGGCT CTGGACATGT TGTCTAGTGA TTTTTCAGAT 
GTACCGACTG TAAAGCCCAC TGTATCTGAT GGCAAAAGCT TCACCCCTGA ACCAACACCA 
CCAACCTTTA AGGCAACCGG TCCGGTTTTA GACAAACTGG CAGACAACGT GCTTTCCAAC 
CTGACTGACC CCAAAGCCAA AGACAGCAAA GCAAAGAAAC AGGCAGTGGA AGACTCTTCA 
GCCACAGATC TGCTGTCCAG TAAACCGAGC TCAGATGTGG TGCCTTCAGC TTCCACAAAG 
GCTGGAAAAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_005161031.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161088.1 Danio rerio calpastatin (cast), transcript variant X9, mRNA.

Price & Availability↑

CloneID	ODa25609
Clone ID Related Accession (Same CDS sequence)	XM_005161031.4 , XM_005161031.3
Accession Version	XM_005161031.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	calpastatin isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005161031.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTACG CAATGTATTG GGCGCGCAAC AATGGAGACA CTTCATTTCA AAAACAAGAA 
TACGCTCCAC AGACATCAAG GTTCATGTCT CAGCAGGTCA CACCCACCAC AACATCTCAG 
GTCTCCACAG TGAAACCTGC ACAATATGAG AAAGGAGCTA CACAATCGTC TACAGCAGCT 
GCGACTGTCA AATCCGGCGC TGGAGATCCT GCAACCACCC AGAAAGGATC TTCTCAAGTC 
AAACCTCAGG TCACAGATTC TAAATCAACA CCTGCATCTT CTGCTAAAGT CTCAGCTGTG 
AGCTCTGGGA CTGGAGTTGC TGGTACAACC GGTGGAGCAG GAGTGAAACC AGCAGATCCT 
GCAAAAGATA CGCCTCAGAG CACCACCATT CCTTCAACCA AACTGGGACC TGCTAAGGTG 
GATCCGGCTG CTGGGAAGCC CTCTGGCTCA GCTGGTGCCC AAAAACAGAC AAGTGCTCAA 
AAGGTGCAGG TTGAAGTGGG TCCTTCAGCA GCAAAAGCTG ACGATCCGTT TGATGCCCTG 
TCTGGCACAT TACCATCATC ACAACCTTTG GCTCCTAAAG TCCACTACAC TGGACCTGAG 
ATCAAAGAGC CCAAACTGAA ATCAGAGAAA GGTGTTTTGT GTGGTGAGCG AGATGACACG 
CTGCCGCCCG GATACAGAAA GGAAGACCTG GAAAAGAAGC ATGGAGCTGG AGTTCCAGCT 
GGAGTTCCGG AGAAGCCTAA AGAAGTTCCC AAGCCCATAA GCACAGATGA TGCACTGGAA 
TCTCTCTCCT CTACGTTTGT GTCTCCAGCT CAAGCTGCTC CAAAGAAGAC AGACGTGAAA 
ACTGAAACAA TAGGAGCAGT GGATGTTAAA TCGGCAGGCA CCACTAACTT TGCTCCCCCT 
CCACCATCTC AGCAGAAACA ACCGGCGTCG TCTCAAGCTG CTGCTGTAAC CAAATCACCT 
GCTCCACCGG CAGACAAGAA AGCTAAAATG GAGACTCATG CACAACCAGC CAAAGCAAAG 
ACAGAGTCGG ACAGCTCCAT ATCGCTGGAC GCTCTCGATG CTTTGAGCGA CACACTGGGG 
GCTTCTAAAC CACCCCAAAA ATTACCAGAA CTCAAACCTG GACAGATAGT TGATGAGAAG 
AAACAGACCT CAGAGAAAGG TGTTCGTGTT GGAGAAAGAG ACGACACACT TCCACCACAA 
TACAGATTCA AAGAGGAAGA TCTCAAGAAA TATCCTCCAC CTGCGAAAGA GCCTTCACTG 
AACACAGATG ATGCGATGGA CATACTTTCA GGAGATTTCA CGACCCCTGC ATCGGCACCT 
GTTGTCAAAG CTCCTGTTCC TCCTGCACAG GACACAAAGA AGCATGCTGC AGTGCCTGAG 
AAGACCAAAG ATATTCCCAA GACTAAAGCT GATGCTCTGT CAAGTGATTT CGTGGCTCCG 
GCACATTCTG CTCCTAAGGT TTCTTCAGCT GCTCCTAAAA CCATCCCTCC AGGCCCACCA 
CAAACCCCAG ATGAGGATCC TTTCAGTGCT TTGGGCGACA CTCTTTCTGC CCCAGAACCA 
CCAAAAAAAC AACCTGACCT GAAACCTAAG GACATCGTAC ATGAGAAGGA TGTGACGTCA 
AAGAAAGGAG TTCGTGTTGG AGAAAGAGAC GACACACTCC CACCAGAATA CAGATTCAAA 
GAGGAAGATC TCAAGAAATA TCCTCCTCCT CAGAAAGATC CTTCCTTAGA TGCTAATGAA 
GCTCTGGATA TTCTGTCTGG GGATTTTACA ACTCCTTCCG CACCATCGGC TGTTTCCCCT 
GCCTCTAAAT CCTCTGCCAA GTCTTCAGAT TCAGCTTTAG ATGCTCTTGC TGGTGATTTC 
GTCGCTTCTT CTTCTGCGTC TTCAGTTCAT TCTGCCGTCT CTGGCCCCCC ACGCTCCGAC 
AGACTGTTGT CAGACGGAAC CTCATCAGCT CTAGATGCCC TGTCAGAAAC TCTGGGAGAC 
ATTAAAGCAG CCCCTGAACC CGCCCCTGTC CTACCTAAAA CTGTCGTTAA GGAGAAGACG 
GTCATAGAAG AGAGAGTGAG TAAACCGGGT GAGAGAGACG ACTCCCTTCC ACCAGATCAG 
CGGTTCACTG CGGAGGATCG CAAGGCATTT GAAGAAGCAA AGAAGAAAGA CGCCAAACCA 
AAACAGCCAT CAATGGACGA TTCAGCGGCT CTGGACATGT TGTCTAGTGA TTTTTCAGAT 
GTACCGACTG TAAAGCCCAC TGTATCTGAT GGCAAAAGCT TCACCCCTGA ACCAACACCA 
CCAACCTTTA AGGCAACCGG TCCGGTTTTA GACAAACTGG CAGACAACGT GCTTTCCAAC 
CTGACTGACC CCAAAGCCAA AGACAGCAAA GCAAAGAAAC AGGCAGTGGA AGACTCTTCA 
GCCACAGATC TGCTGTCCAG TAAACCGAGC TCAGATGTGG TGCCTTCAGC TTCCACAAAG 
GCTGGAAAAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161088.1
CDS	529..3003
Translation	MAYAMYWARNNGDTSFQKQEYAPQTSRFMSQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQVTDSKSTPASSAKVSAVSSGTGVAGTTGGAGVKPADPAKDTPQSTTIPSTKLGPAKVDPAAGKPSGSAGAQKQTSAQKVQVEVGPSAAKADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_005161031.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161031.3

ORF Insert Sequence:

ATGGCGTACG CAATGTATTG GGCGCGCAAC AATGGAGACA CTTCATTTCA AAAACAAGAA 
TACGCTCCAC AGACATCAAG GTTCATGTCT CAGCAGGTCA CACCCACCAC AACATCTCAG 
GTCTCCACAG TGAAACCTGC ACAATATGAG AAAGGAGCTA CACAATCGTC TACAGCAGCT 
GCGACTGTCA AATCCGGCGC TGGAGATCCT GCAACCACCC AGAAAGGATC TTCTCAAGTC 
AAACCTCAGG TCACAGATTC TAAATCAACA CCTGCATCTT CTGCTAAAGT CTCAGCTGTG 
AGCTCTGGGA CTGGAGTTGC TGGTACAACC GGTGGAGCAG GAGTGAAACC AGCAGATCCT 
GCAAAAGATA CGCCTCAGAG CACCACCATT CCTTCAACCA AACTGGGACC TGCTAAGGTG 
GATCCGGCTG CTGGGAAGCC CTCTGGCTCA GCTGGTGCCC AAAAACAGAC AAGTGCTCAA 
AAGGTGCAGG TTGAAGTGGG TCCTTCAGCA GCAAAAGCTG ACGATCCGTT TGATGCCCTG 
TCTGGCACAT TACCATCATC ACAACCTTTG GCTCCTAAAG TCCACTACAC TGGACCTGAG 
ATCAAAGAGC CCAAACTGAA ATCAGAGAAA GGTGTTTTGT GTGGTGAGCG AGATGACACG 
CTGCCGCCCG GATACAGAAA GGAAGACCTG GAAAAGAAGC ATGGAGCTGG AGTTCCAGCT 
GGAGTTCCGG AGAAGCCTAA AGAAGTTCCC AAGCCCATAA GCACAGATGA TGCACTGGAA 
TCTCTCTCCT CTACGTTTGT GTCTCCAGCT CAAGCTGCTC CAAAGAAGAC AGACGTGAAA 
ACTGAAACAA TAGGAGCAGT GGATGTTAAA TCGGCAGGCA CCACTAACTT TGCTCCCCCT 
CCACCATCTC AGCAGAAACA ACCGGCGTCG TCTCAAGCTG CTGCTGTAAC CAAATCACCT 
GCTCCACCGG CAGACAAGAA AGCTAAAATG GAGACTCATG CACAACCAGC CAAAGCAAAG 
ACAGAGTCGG ACAGCTCCAT ATCGCTGGAC GCTCTCGATG CTTTGAGCGA CACACTGGGG 
GCTTCTAAAC CACCCCAAAA ATTACCAGAA CTCAAACCTG GACAGATAGT TGATGAGAAG 
AAACAGACCT CAGAGAAAGG TGTTCGTGTT GGAGAAAGAG ACGACACACT TCCACCACAA 
TACAGATTCA AAGAGGAAGA TCTCAAGAAA TATCCTCCAC CTGCGAAAGA GCCTTCACTG 
AACACAGATG ATGCGATGGA CATACTTTCA GGAGATTTCA CGACCCCTGC ATCGGCACCT 
GTTGTCAAAG CTCCTGTTCC TCCTGCACAG GACACAAAGA AGCATGCTGC AGTGCCTGAG 
AAGACCAAAG ATATTCCCAA GACTAAAGCT GATGCTCTGT CAAGTGATTT CGTGGCTCCG 
GCACATTCTG CTCCTAAGGT TTCTTCAGCT GCTCCTAAAA CCATCCCTCC AGGCCCACCA 
CAAACCCCAG ATGAGGATCC TTTCAGTGCT TTGGGCGACA CTCTTTCTGC CCCAGAACCA 
CCAAAAAAAC AACCTGACCT GAAACCTAAG GACATCGTAC ATGAGAAGGA TGTGACGTCA 
AAGAAAGGAG TTCGTGTTGG AGAAAGAGAC GACACACTCC CACCAGAATA CAGATTCAAA 
GAGGAAGATC TCAAGAAATA TCCTCCTCCT CAGAAAGATC CTTCCTTAGA TGCTAATGAA 
GCTCTGGATA TTCTGTCTGG GGATTTTACA ACTCCTTCCG CACCATCGGC TGTTTCCCCT 
GCCTCTAAAT CCTCTGCCAA GTCTTCAGAT TCAGCTTTAG ATGCTCTTGC TGGTGATTTC 
GTCGCTTCTT CTTCTGCGTC TTCAGTTCAT TCTGCCGTCT CTGGCCCCCC ACGCTCCGAC 
AGACTGTTGT CAGACGGAAC CTCATCAGCT CTAGATGCCC TGTCAGAAAC TCTGGGAGAC 
ATTAAAGCAG CCCCTGAACC CGCCCCTGTC CTACCTAAAA CTGTCGTTAA GGAGAAGACG 
GTCATAGAAG AGAGAGTGAG TAAACCGGGT GAGAGAGACG ACTCCCTTCC ACCAGATCAG 
CGGTTCACTG CGGAGGATCG CAAGGCATTT GAAGAAGCAA AGAAGAAAGA CGCCAAACCA 
AAACAGCCAT CAATGGACGA TTCAGCGGCT CTGGACATGT TGTCTAGTGA TTTTTCAGAT 
GTACCGACTG TAAAGCCCAC TGTATCTGAT GGCAAAAGCT TCACCCCTGA ACCAACACCA 
CCAACCTTTA AGGCAACCGG TCCGGTTTTA GACAAACTGG CAGACAACGT GCTTTCCAAC 
CTGACTGACC CCAAAGCCAA AGACAGCAAA GCAAAGAAAC AGGCAGTGGA AGACTCTTCA 
GCCACAGATC TGCTGTCCAG TAAACCGAGC TCAGATGTGG TGCCTTCAGC TTCCACAAAG 
GCTGGAAAAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_005161034.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161091.1 Danio rerio calpastatin (cast), transcript variant X13, mRNA.

Price & Availability↑

CloneID	ODa25611
Clone ID Related Accession (Same CDS sequence)	XM_005161034.4 , XM_005161033.3 , XM_005161033.4 , XM_005161034.3
Accession Version	XM_005161034.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calpastatin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005161034.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC AGGTCACACC CACCACAACA TCTCAGGTCT CCACAGTGAA ACCTGCACAA 
TATGAGAAAG GAGCTACACA ATCGTCTACA GCAGCTGCGA CTGTCAAATC CGGCGCTGGA 
GATCCTGCAA CCACCCAGAA AGGATCTTCT CAAGTCAAAC CTCAGGTCAC AGATTCTAAA 
TCAACACCTG CATCTTCTGC TAAAGTCTCA GCTGTGAGCT CTGGGACTGG AGTTGCTGGT 
ACAACCGGTG GAGCAGGAGT GAAACCAGCA GATCCTGCAA AAGATACGCC TCAGAGCACC 
ACCATTCCTT CAACCAAACT GGGACCTGCT AAGGTGGATC CGGCTGCTGG GAAGCCCTCT 
GGCTCAGCTG GTGCCCAAAA ACAGACAAGT GCTCAAAAGG TGCAGGTTGA AGTGGGTCCT 
TCAGCAGCAA AAGTCAGTGC AGATGCTGAC GATCCGTTTG ATGCCCTGTC TGGCACATTA 
CCATCATCAC AACCTTTGGC TCCTAAAGTC CACTACACTG GACCTGAGAT CAAAGAGCCC 
AAACTGAAAT CAGAGAAAGG TGTTTTGTGT GGTGAGCGAG ATGACACGCT GCCGCCCGGA 
TACAGAAAGG AAGACCTGGA AAAGAAGCAT GGAGCTGGAG TTCCAGCTGG AGTTCCGGAG 
AAGCCTAAAG AAGTTCCCAA GCCCATAAGC ACAGATGATG CACTGGAATC TCTCTCCTCT 
ACGTTTGTGT CTCCAGCTCA AGCTGCTCCA AAGAAGACAG ACGTGAAAAC TGAAACAATA 
GGAGCAGTGG ATGTTAAATC GGCAGGCACC ACTAACTTTG CTCCCCCTCC ACCATCTCAG 
CAGAAACAAC CGGCGTCGTC TCAAGCTGCT GCTGTAACCA AATCACCTGC TCCACCGGCA 
GACAAGAAAG CTAAAATGGA GACTCATGCA CAACCAGCCA AAGCAAAGAC AGAGTCGGAC 
AGCTCCATAT CGCTGGACGC TCTCGATGCT TTGAGCGACA CACTGGGGGC TTCTAAACCA 
CCCCAAAAAT TACCAGAACT CAAACCTGGA CAGATAGTTG ATGAGAAGAA ACAGACCTCA 
GAGAAAGGTG TTCGTGTTGG AGAAAGAGAC GACACACTTC CACCACAATA CAGATTCAAA 
GAGGAAGATC TCAAGAAATA TCCTCCACCT GCGAAAGAGC CTTCACTGAA CACAGATGAT 
GCGATGGACA TACTTTCAGG AGATTTCACG ACCCCTGCAT CGGCACCTGT TGTCAAAGCT 
CCTGTTCCTC CTGCACAGGA CACAAAGAAG CATGCTGCAG TGCCTGAGAA GACCAAAGAT 
ATTCCCAAGA CTAAAGCTGA TGCTCTGTCA AGTGATTTCG TGGCTCCGGC ACATTCTGCT 
CCTAAGGTTT CTTCAGCTGC TCCTAAAACC ATCCCTCCAG GCCCACCACA AACCCCAGAT 
GAGGATCCTT TCAGTGCTTT GGGCGACACT CTTTCTGCCC CAGAACCACC AAAAAAACAA 
CCTGACCTGA AACCTAAGGA CATCGTACAT GAGAAGGATG TGACGTCAAA GAAAGGAGTT 
CGTGTTGGAG AAAGAGACGA CACACTCCCA CCAGAATACA GATTCAAAGA GGAAGATCTC 
AAGAAATATC CTCCTCCTCA GAAAGATCCT TCCTTAGATG CTAATGAAGC TCTGGATATT 
CTGTCTGGGG ATTTTACAAC TCCTTCCGCA CCATCGGCTG TTTCCCCTGC CTCTAAATCC 
TCTGCCAAGT CTTCAGATTC AGCTTTAGAT GCTCTTGCTG GTGATTTCGT CGCTTCTTCT 
TCTGCGTCTT CAGTTCATTC TGCCGTCTCT GGCCCCCCAC GCTCCGACAG ACTGTTGTCA 
GACGGAACCT CATCAGCTCT AGATGCCCTG TCAGAAACTC TGGGAGACAT TAAAGCAGCC 
CCTGAACCCG CCCCTGTCCT ACCTAAAACT GTCGTTAAGG AGAAGACGGT CATAGAAGAG 
AGAGTGAGTA AACCGGGTGA GAGAGACGAC TCCCTTCCAC CAGATCAGCG GTTCACTGCG 
GAGGATCGCA AGGCATTTGA AGAAGCAAAG AAGAAAGACG CCAAACCAAA ACAGCCATCA 
ATGGACGATT CAGCGGCTCT GGACATGTTG TCTAGTGATT TTTCAGATGT ACCGACTGTA 
AAGCCCACTG TATCTGATGG CAAAAGCTTC ACCCCTGAAC CAACACCACC AACCTTTAAG 
GCAACCGGTC CGGTTTTAGA CAAACTGGCA GACAACGTGC TTTCCAACCT GACTGACCCC 
AAAGCCAAAG ACAGCAAAGC AAAGGGAAAG GGGGCGAAAC CAAAGTCTAA ACCAAAGAAA 
CAGGCAGTGG AAGACTCTTC AGCCACAGAT CTGCTGTCCA GTAAACCGAG CTCAGATGTG 
GTGCCTTCAG CTTCCACAAA GGCTGGAAAA AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161091.1
CDS	94..2529
Translation	MSQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQVTDSKSTPASSAKVSAVSSGTGVAGTTGGAGVKPADPAKDTPQSTTIPSTKLGPAKVDPAAGKPSGSAGAQKQTSAQKVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_005161034.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161034.4

ORF Insert Sequence:

ATGTCTCAGC AGGTCACACC CACCACAACA TCTCAGGTCT CCACAGTGAA ACCTGCACAA 
TATGAGAAAG GAGCTACACA ATCGTCTACA GCAGCTGCGA CTGTCAAATC CGGCGCTGGA 
GATCCTGCAA CCACCCAGAA AGGATCTTCT CAAGTCAAAC CTCAGGTCAC AGATTCTAAA 
TCAACACCTG CATCTTCTGC TAAAGTCTCA GCTGTGAGCT CTGGGACTGG AGTTGCTGGT 
ACAACCGGTG GAGCAGGAGT GAAACCAGCA GATCCTGCAA AAGATACGCC TCAGAGCACC 
ACCATTCCTT CAACCAAACT GGGACCTGCT AAGGTGGATC CGGCTGCTGG GAAGCCCTCT 
GGCTCAGCTG GTGCCCAAAA ACAGACAAGT GCTCAAAAGG TGCAGGTTGA AGTGGGTCCT 
TCAGCAGCAA AAGTCAGTGC AGATGCTGAC GATCCGTTTG ATGCCCTGTC TGGCACATTA 
CCATCATCAC AACCTTTGGC TCCTAAAGTC CACTACACTG GACCTGAGAT CAAAGAGCCC 
AAACTGAAAT CAGAGAAAGG TGTTTTGTGT GGTGAGCGAG ATGACACGCT GCCGCCCGGA 
TACAGAAAGG AAGACCTGGA AAAGAAGCAT GGAGCTGGAG TTCCAGCTGG AGTTCCGGAG 
AAGCCTAAAG AAGTTCCCAA GCCCATAAGC ACAGATGATG CACTGGAATC TCTCTCCTCT 
ACGTTTGTGT CTCCAGCTCA AGCTGCTCCA AAGAAGACAG ACGTGAAAAC TGAAACAATA 
GGAGCAGTGG ATGTTAAATC GGCAGGCACC ACTAACTTTG CTCCCCCTCC ACCATCTCAG 
CAGAAACAAC CGGCGTCGTC TCAAGCTGCT GCTGTAACCA AATCACCTGC TCCACCGGCA 
GACAAGAAAG CTAAAATGGA GACTCATGCA CAACCAGCCA AAGCAAAGAC AGAGTCGGAC 
AGCTCCATAT CGCTGGACGC TCTCGATGCT TTGAGCGACA CACTGGGGGC TTCTAAACCA 
CCCCAAAAAT TACCAGAACT CAAACCTGGA CAGATAGTTG ATGAGAAGAA ACAGACCTCA 
GAGAAAGGTG TTCGTGTTGG AGAAAGAGAC GACACACTTC CACCACAATA CAGATTCAAA 
GAGGAAGATC TCAAGAAATA TCCTCCACCT GCGAAAGAGC CTTCACTGAA CACAGATGAT 
GCGATGGACA TACTTTCAGG AGATTTCACG ACCCCTGCAT CGGCACCTGT TGTCAAAGCT 
CCTGTTCCTC CTGCACAGGA CACAAAGAAG CATGCTGCAG TGCCTGAGAA GACCAAAGAT 
ATTCCCAAGA CTAAAGCTGA TGCTCTGTCA AGTGATTTCG TGGCTCCGGC ACATTCTGCT 
CCTAAGGTTT CTTCAGCTGC TCCTAAAACC ATCCCTCCAG GCCCACCACA AACCCCAGAT 
GAGGATCCTT TCAGTGCTTT GGGCGACACT CTTTCTGCCC CAGAACCACC AAAAAAACAA 
CCTGACCTGA AACCTAAGGA CATCGTACAT GAGAAGGATG TGACGTCAAA GAAAGGAGTT 
CGTGTTGGAG AAAGAGACGA CACACTCCCA CCAGAATACA GATTCAAAGA GGAAGATCTC 
AAGAAATATC CTCCTCCTCA GAAAGATCCT TCCTTAGATG CTAATGAAGC TCTGGATATT 
CTGTCTGGGG ATTTTACAAC TCCTTCCGCA CCATCGGCTG TTTCCCCTGC CTCTAAATCC 
TCTGCCAAGT CTTCAGATTC AGCTTTAGAT GCTCTTGCTG GTGATTTCGT CGCTTCTTCT 
TCTGCGTCTT CAGTTCATTC TGCCGTCTCT GGCCCCCCAC GCTCCGACAG ACTGTTGTCA 
GACGGAACCT CATCAGCTCT AGATGCCCTG TCAGAAACTC TGGGAGACAT TAAAGCAGCC 
CCTGAACCCG CCCCTGTCCT ACCTAAAACT GTCGTTAAGG AGAAGACGGT CATAGAAGAG 
AGAGTGAGTA AACCGGGTGA GAGAGACGAC TCCCTTCCAC CAGATCAGCG GTTCACTGCG 
GAGGATCGCA AGGCATTTGA AGAAGCAAAG AAGAAAGACG CCAAACCAAA ACAGCCATCA 
ATGGACGATT CAGCGGCTCT GGACATGTTG TCTAGTGATT TTTCAGATGT ACCGACTGTA 
AAGCCCACTG TATCTGATGG CAAAAGCTTC ACCCCTGAAC CAACACCACC AACCTTTAAG 
GCAACCGGTC CGGTTTTAGA CAAACTGGCA GACAACGTGC TTTCCAACCT GACTGACCCC 
AAAGCCAAAG ACAGCAAAGC AAAGGGAAAG GGGGCGAAAC CAAAGTCTAA ACCAAAGAAA 
CAGGCAGTGG AAGACTCTTC AGCCACAGAT CTGCTGTCCA GTAAACCGAG CTCAGATGTG 
GTGCCTTCAG CTTCCACAAA GGCTGGAAAA AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_005161033.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161090.1 Danio rerio calpastatin (cast), transcript variant X10, mRNA.

Price & Availability↑

CloneID	ODa25611
Clone ID Related Accession (Same CDS sequence)	XM_005161034.4 , XM_005161033.3 , XM_005161033.4 , XM_005161034.3
Accession Version	XM_005161033.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	calpastatin isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005161033.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC AGGTCACACC CACCACAACA TCTCAGGTCT CCACAGTGAA ACCTGCACAA 
TATGAGAAAG GAGCTACACA ATCGTCTACA GCAGCTGCGA CTGTCAAATC CGGCGCTGGA 
GATCCTGCAA CCACCCAGAA AGGATCTTCT CAAGTCAAAC CTCAGGTCAC AGATTCTAAA 
TCAACACCTG CATCTTCTGC TAAAGTCTCA GCTGTGAGCT CTGGGACTGG AGTTGCTGGT 
ACAACCGGTG GAGCAGGAGT GAAACCAGCA GATCCTGCAA AAGATACGCC TCAGAGCACC 
ACCATTCCTT CAACCAAACT GGGACCTGCT AAGGTGGATC CGGCTGCTGG GAAGCCCTCT 
GGCTCAGCTG GTGCCCAAAA ACAGACAAGT GCTCAAAAGG TGCAGGTTGA AGTGGGTCCT 
TCAGCAGCAA AAGTCAGTGC AGATGCTGAC GATCCGTTTG ATGCCCTGTC TGGCACATTA 
CCATCATCAC AACCTTTGGC TCCTAAAGTC CACTACACTG GACCTGAGAT CAAAGAGCCC 
AAACTGAAAT CAGAGAAAGG TGTTTTGTGT GGTGAGCGAG ATGACACGCT GCCGCCCGGA 
TACAGAAAGG AAGACCTGGA AAAGAAGCAT GGAGCTGGAG TTCCAGCTGG AGTTCCGGAG 
AAGCCTAAAG AAGTTCCCAA GCCCATAAGC ACAGATGATG CACTGGAATC TCTCTCCTCT 
ACGTTTGTGT CTCCAGCTCA AGCTGCTCCA AAGAAGACAG ACGTGAAAAC TGAAACAATA 
GGAGCAGTGG ATGTTAAATC GGCAGGCACC ACTAACTTTG CTCCCCCTCC ACCATCTCAG 
CAGAAACAAC CGGCGTCGTC TCAAGCTGCT GCTGTAACCA AATCACCTGC TCCACCGGCA 
GACAAGAAAG CTAAAATGGA GACTCATGCA CAACCAGCCA AAGCAAAGAC AGAGTCGGAC 
AGCTCCATAT CGCTGGACGC TCTCGATGCT TTGAGCGACA CACTGGGGGC TTCTAAACCA 
CCCCAAAAAT TACCAGAACT CAAACCTGGA CAGATAGTTG ATGAGAAGAA ACAGACCTCA 
GAGAAAGGTG TTCGTGTTGG AGAAAGAGAC GACACACTTC CACCACAATA CAGATTCAAA 
GAGGAAGATC TCAAGAAATA TCCTCCACCT GCGAAAGAGC CTTCACTGAA CACAGATGAT 
GCGATGGACA TACTTTCAGG AGATTTCACG ACCCCTGCAT CGGCACCTGT TGTCAAAGCT 
CCTGTTCCTC CTGCACAGGA CACAAAGAAG CATGCTGCAG TGCCTGAGAA GACCAAAGAT 
ATTCCCAAGA CTAAAGCTGA TGCTCTGTCA AGTGATTTCG TGGCTCCGGC ACATTCTGCT 
CCTAAGGTTT CTTCAGCTGC TCCTAAAACC ATCCCTCCAG GCCCACCACA AACCCCAGAT 
GAGGATCCTT TCAGTGCTTT GGGCGACACT CTTTCTGCCC CAGAACCACC AAAAAAACAA 
CCTGACCTGA AACCTAAGGA CATCGTACAT GAGAAGGATG TGACGTCAAA GAAAGGAGTT 
CGTGTTGGAG AAAGAGACGA CACACTCCCA CCAGAATACA GATTCAAAGA GGAAGATCTC 
AAGAAATATC CTCCTCCTCA GAAAGATCCT TCCTTAGATG CTAATGAAGC TCTGGATATT 
CTGTCTGGGG ATTTTACAAC TCCTTCCGCA CCATCGGCTG TTTCCCCTGC CTCTAAATCC 
TCTGCCAAGT CTTCAGATTC AGCTTTAGAT GCTCTTGCTG GTGATTTCGT CGCTTCTTCT 
TCTGCGTCTT CAGTTCATTC TGCCGTCTCT GGCCCCCCAC GCTCCGACAG ACTGTTGTCA 
GACGGAACCT CATCAGCTCT AGATGCCCTG TCAGAAACTC TGGGAGACAT TAAAGCAGCC 
CCTGAACCCG CCCCTGTCCT ACCTAAAACT GTCGTTAAGG AGAAGACGGT CATAGAAGAG 
AGAGTGAGTA AACCGGGTGA GAGAGACGAC TCCCTTCCAC CAGATCAGCG GTTCACTGCG 
GAGGATCGCA AGGCATTTGA AGAAGCAAAG AAGAAAGACG CCAAACCAAA ACAGCCATCA 
ATGGACGATT CAGCGGCTCT GGACATGTTG TCTAGTGATT TTTCAGATGT ACCGACTGTA 
AAGCCCACTG TATCTGATGG CAAAAGCTTC ACCCCTGAAC CAACACCACC AACCTTTAAG 
GCAACCGGTC CGGTTTTAGA CAAACTGGCA GACAACGTGC TTTCCAACCT GACTGACCCC 
AAAGCCAAAG ACAGCAAAGC AAAGGGAAAG GGGGCGAAAC CAAAGTCTAA ACCAAAGAAA 
CAGGCAGTGG AAGACTCTTC AGCCACAGAT CTGCTGTCCA GTAAACCGAG CTCAGATGTG 
GTGCCTTCAG CTTCCACAAA GGCTGGAAAA AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161090.1
CDS	130..2565
Translation	MSQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQVTDSKSTPASSAKVSAVSSGTGVAGTTGGAGVKPADPAKDTPQSTTIPSTKLGPAKVDPAAGKPSGSAGAQKQTSAQKVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_005161033.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161033.3

ORF Insert Sequence:

ATGTCTCAGC AGGTCACACC CACCACAACA TCTCAGGTCT CCACAGTGAA ACCTGCACAA 
TATGAGAAAG GAGCTACACA ATCGTCTACA GCAGCTGCGA CTGTCAAATC CGGCGCTGGA 
GATCCTGCAA CCACCCAGAA AGGATCTTCT CAAGTCAAAC CTCAGGTCAC AGATTCTAAA 
TCAACACCTG CATCTTCTGC TAAAGTCTCA GCTGTGAGCT CTGGGACTGG AGTTGCTGGT 
ACAACCGGTG GAGCAGGAGT GAAACCAGCA GATCCTGCAA AAGATACGCC TCAGAGCACC 
ACCATTCCTT CAACCAAACT GGGACCTGCT AAGGTGGATC CGGCTGCTGG GAAGCCCTCT 
GGCTCAGCTG GTGCCCAAAA ACAGACAAGT GCTCAAAAGG TGCAGGTTGA AGTGGGTCCT 
TCAGCAGCAA AAGTCAGTGC AGATGCTGAC GATCCGTTTG ATGCCCTGTC TGGCACATTA 
CCATCATCAC AACCTTTGGC TCCTAAAGTC CACTACACTG GACCTGAGAT CAAAGAGCCC 
AAACTGAAAT CAGAGAAAGG TGTTTTGTGT GGTGAGCGAG ATGACACGCT GCCGCCCGGA 
TACAGAAAGG AAGACCTGGA AAAGAAGCAT GGAGCTGGAG TTCCAGCTGG AGTTCCGGAG 
AAGCCTAAAG AAGTTCCCAA GCCCATAAGC ACAGATGATG CACTGGAATC TCTCTCCTCT 
ACGTTTGTGT CTCCAGCTCA AGCTGCTCCA AAGAAGACAG ACGTGAAAAC TGAAACAATA 
GGAGCAGTGG ATGTTAAATC GGCAGGCACC ACTAACTTTG CTCCCCCTCC ACCATCTCAG 
CAGAAACAAC CGGCGTCGTC TCAAGCTGCT GCTGTAACCA AATCACCTGC TCCACCGGCA 
GACAAGAAAG CTAAAATGGA GACTCATGCA CAACCAGCCA AAGCAAAGAC AGAGTCGGAC 
AGCTCCATAT CGCTGGACGC TCTCGATGCT TTGAGCGACA CACTGGGGGC TTCTAAACCA 
CCCCAAAAAT TACCAGAACT CAAACCTGGA CAGATAGTTG ATGAGAAGAA ACAGACCTCA 
GAGAAAGGTG TTCGTGTTGG AGAAAGAGAC GACACACTTC CACCACAATA CAGATTCAAA 
GAGGAAGATC TCAAGAAATA TCCTCCACCT GCGAAAGAGC CTTCACTGAA CACAGATGAT 
GCGATGGACA TACTTTCAGG AGATTTCACG ACCCCTGCAT CGGCACCTGT TGTCAAAGCT 
CCTGTTCCTC CTGCACAGGA CACAAAGAAG CATGCTGCAG TGCCTGAGAA GACCAAAGAT 
ATTCCCAAGA CTAAAGCTGA TGCTCTGTCA AGTGATTTCG TGGCTCCGGC ACATTCTGCT 
CCTAAGGTTT CTTCAGCTGC TCCTAAAACC ATCCCTCCAG GCCCACCACA AACCCCAGAT 
GAGGATCCTT TCAGTGCTTT GGGCGACACT CTTTCTGCCC CAGAACCACC AAAAAAACAA 
CCTGACCTGA AACCTAAGGA CATCGTACAT GAGAAGGATG TGACGTCAAA GAAAGGAGTT 
CGTGTTGGAG AAAGAGACGA CACACTCCCA CCAGAATACA GATTCAAAGA GGAAGATCTC 
AAGAAATATC CTCCTCCTCA GAAAGATCCT TCCTTAGATG CTAATGAAGC TCTGGATATT 
CTGTCTGGGG ATTTTACAAC TCCTTCCGCA CCATCGGCTG TTTCCCCTGC CTCTAAATCC 
TCTGCCAAGT CTTCAGATTC AGCTTTAGAT GCTCTTGCTG GTGATTTCGT CGCTTCTTCT 
TCTGCGTCTT CAGTTCATTC TGCCGTCTCT GGCCCCCCAC GCTCCGACAG ACTGTTGTCA 
GACGGAACCT CATCAGCTCT AGATGCCCTG TCAGAAACTC TGGGAGACAT TAAAGCAGCC 
CCTGAACCCG CCCCTGTCCT ACCTAAAACT GTCGTTAAGG AGAAGACGGT CATAGAAGAG 
AGAGTGAGTA AACCGGGTGA GAGAGACGAC TCCCTTCCAC CAGATCAGCG GTTCACTGCG 
GAGGATCGCA AGGCATTTGA AGAAGCAAAG AAGAAAGACG CCAAACCAAA ACAGCCATCA 
ATGGACGATT CAGCGGCTCT GGACATGTTG TCTAGTGATT TTTCAGATGT ACCGACTGTA 
AAGCCCACTG TATCTGATGG CAAAAGCTTC ACCCCTGAAC CAACACCACC AACCTTTAAG 
GCAACCGGTC CGGTTTTAGA CAAACTGGCA GACAACGTGC TTTCCAACCT GACTGACCCC 
AAAGCCAAAG ACAGCAAAGC AAAGGGAAAG GGGGCGAAAC CAAAGTCTAA ACCAAAGAAA 
CAGGCAGTGG AAGACTCTTC AGCCACAGAT CTGCTGTCCA GTAAACCGAG CTCAGATGTG 
GTGCCTTCAG CTTCCACAAA GGCTGGAAAA AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_005161033.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161090.1 Danio rerio calpastatin (cast), transcript variant X12, mRNA.

Price & Availability↑

CloneID	ODa25611
Clone ID Related Accession (Same CDS sequence)	XM_005161034.4 , XM_005161033.3 , XM_005161033.4 , XM_005161034.3
Accession Version	XM_005161033.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calpastatin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005161033.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC AGGTCACACC CACCACAACA TCTCAGGTCT CCACAGTGAA ACCTGCACAA 
TATGAGAAAG GAGCTACACA ATCGTCTACA GCAGCTGCGA CTGTCAAATC CGGCGCTGGA 
GATCCTGCAA CCACCCAGAA AGGATCTTCT CAAGTCAAAC CTCAGGTCAC AGATTCTAAA 
TCAACACCTG CATCTTCTGC TAAAGTCTCA GCTGTGAGCT CTGGGACTGG AGTTGCTGGT 
ACAACCGGTG GAGCAGGAGT GAAACCAGCA GATCCTGCAA AAGATACGCC TCAGAGCACC 
ACCATTCCTT CAACCAAACT GGGACCTGCT AAGGTGGATC CGGCTGCTGG GAAGCCCTCT 
GGCTCAGCTG GTGCCCAAAA ACAGACAAGT GCTCAAAAGG TGCAGGTTGA AGTGGGTCCT 
TCAGCAGCAA AAGTCAGTGC AGATGCTGAC GATCCGTTTG ATGCCCTGTC TGGCACATTA 
CCATCATCAC AACCTTTGGC TCCTAAAGTC CACTACACTG GACCTGAGAT CAAAGAGCCC 
AAACTGAAAT CAGAGAAAGG TGTTTTGTGT GGTGAGCGAG ATGACACGCT GCCGCCCGGA 
TACAGAAAGG AAGACCTGGA AAAGAAGCAT GGAGCTGGAG TTCCAGCTGG AGTTCCGGAG 
AAGCCTAAAG AAGTTCCCAA GCCCATAAGC ACAGATGATG CACTGGAATC TCTCTCCTCT 
ACGTTTGTGT CTCCAGCTCA AGCTGCTCCA AAGAAGACAG ACGTGAAAAC TGAAACAATA 
GGAGCAGTGG ATGTTAAATC GGCAGGCACC ACTAACTTTG CTCCCCCTCC ACCATCTCAG 
CAGAAACAAC CGGCGTCGTC TCAAGCTGCT GCTGTAACCA AATCACCTGC TCCACCGGCA 
GACAAGAAAG CTAAAATGGA GACTCATGCA CAACCAGCCA AAGCAAAGAC AGAGTCGGAC 
AGCTCCATAT CGCTGGACGC TCTCGATGCT TTGAGCGACA CACTGGGGGC TTCTAAACCA 
CCCCAAAAAT TACCAGAACT CAAACCTGGA CAGATAGTTG ATGAGAAGAA ACAGACCTCA 
GAGAAAGGTG TTCGTGTTGG AGAAAGAGAC GACACACTTC CACCACAATA CAGATTCAAA 
GAGGAAGATC TCAAGAAATA TCCTCCACCT GCGAAAGAGC CTTCACTGAA CACAGATGAT 
GCGATGGACA TACTTTCAGG AGATTTCACG ACCCCTGCAT CGGCACCTGT TGTCAAAGCT 
CCTGTTCCTC CTGCACAGGA CACAAAGAAG CATGCTGCAG TGCCTGAGAA GACCAAAGAT 
ATTCCCAAGA CTAAAGCTGA TGCTCTGTCA AGTGATTTCG TGGCTCCGGC ACATTCTGCT 
CCTAAGGTTT CTTCAGCTGC TCCTAAAACC ATCCCTCCAG GCCCACCACA AACCCCAGAT 
GAGGATCCTT TCAGTGCTTT GGGCGACACT CTTTCTGCCC CAGAACCACC AAAAAAACAA 
CCTGACCTGA AACCTAAGGA CATCGTACAT GAGAAGGATG TGACGTCAAA GAAAGGAGTT 
CGTGTTGGAG AAAGAGACGA CACACTCCCA CCAGAATACA GATTCAAAGA GGAAGATCTC 
AAGAAATATC CTCCTCCTCA GAAAGATCCT TCCTTAGATG CTAATGAAGC TCTGGATATT 
CTGTCTGGGG ATTTTACAAC TCCTTCCGCA CCATCGGCTG TTTCCCCTGC CTCTAAATCC 
TCTGCCAAGT CTTCAGATTC AGCTTTAGAT GCTCTTGCTG GTGATTTCGT CGCTTCTTCT 
TCTGCGTCTT CAGTTCATTC TGCCGTCTCT GGCCCCCCAC GCTCCGACAG ACTGTTGTCA 
GACGGAACCT CATCAGCTCT AGATGCCCTG TCAGAAACTC TGGGAGACAT TAAAGCAGCC 
CCTGAACCCG CCCCTGTCCT ACCTAAAACT GTCGTTAAGG AGAAGACGGT CATAGAAGAG 
AGAGTGAGTA AACCGGGTGA GAGAGACGAC TCCCTTCCAC CAGATCAGCG GTTCACTGCG 
GAGGATCGCA AGGCATTTGA AGAAGCAAAG AAGAAAGACG CCAAACCAAA ACAGCCATCA 
ATGGACGATT CAGCGGCTCT GGACATGTTG TCTAGTGATT TTTCAGATGT ACCGACTGTA 
AAGCCCACTG TATCTGATGG CAAAAGCTTC ACCCCTGAAC CAACACCACC AACCTTTAAG 
GCAACCGGTC CGGTTTTAGA CAAACTGGCA GACAACGTGC TTTCCAACCT GACTGACCCC 
AAAGCCAAAG ACAGCAAAGC AAAGGGAAAG GGGGCGAAAC CAAAGTCTAA ACCAAAGAAA 
CAGGCAGTGG AAGACTCTTC AGCCACAGAT CTGCTGTCCA GTAAACCGAG CTCAGATGTG 
GTGCCTTCAG CTTCCACAAA GGCTGGAAAA AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161090.1
CDS	149..2584
Translation	MSQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQVTDSKSTPASSAKVSAVSSGTGVAGTTGGAGVKPADPAKDTPQSTTIPSTKLGPAKVDPAAGKPSGSAGAQKQTSAQKVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_005161033.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161033.4

ORF Insert Sequence:

ATGTCTCAGC AGGTCACACC CACCACAACA TCTCAGGTCT CCACAGTGAA ACCTGCACAA 
TATGAGAAAG GAGCTACACA ATCGTCTACA GCAGCTGCGA CTGTCAAATC CGGCGCTGGA 
GATCCTGCAA CCACCCAGAA AGGATCTTCT CAAGTCAAAC CTCAGGTCAC AGATTCTAAA 
TCAACACCTG CATCTTCTGC TAAAGTCTCA GCTGTGAGCT CTGGGACTGG AGTTGCTGGT 
ACAACCGGTG GAGCAGGAGT GAAACCAGCA GATCCTGCAA AAGATACGCC TCAGAGCACC 
ACCATTCCTT CAACCAAACT GGGACCTGCT AAGGTGGATC CGGCTGCTGG GAAGCCCTCT 
GGCTCAGCTG GTGCCCAAAA ACAGACAAGT GCTCAAAAGG TGCAGGTTGA AGTGGGTCCT 
TCAGCAGCAA AAGTCAGTGC AGATGCTGAC GATCCGTTTG ATGCCCTGTC TGGCACATTA 
CCATCATCAC AACCTTTGGC TCCTAAAGTC CACTACACTG GACCTGAGAT CAAAGAGCCC 
AAACTGAAAT CAGAGAAAGG TGTTTTGTGT GGTGAGCGAG ATGACACGCT GCCGCCCGGA 
TACAGAAAGG AAGACCTGGA AAAGAAGCAT GGAGCTGGAG TTCCAGCTGG AGTTCCGGAG 
AAGCCTAAAG AAGTTCCCAA GCCCATAAGC ACAGATGATG CACTGGAATC TCTCTCCTCT 
ACGTTTGTGT CTCCAGCTCA AGCTGCTCCA AAGAAGACAG ACGTGAAAAC TGAAACAATA 
GGAGCAGTGG ATGTTAAATC GGCAGGCACC ACTAACTTTG CTCCCCCTCC ACCATCTCAG 
CAGAAACAAC CGGCGTCGTC TCAAGCTGCT GCTGTAACCA AATCACCTGC TCCACCGGCA 
GACAAGAAAG CTAAAATGGA GACTCATGCA CAACCAGCCA AAGCAAAGAC AGAGTCGGAC 
AGCTCCATAT CGCTGGACGC TCTCGATGCT TTGAGCGACA CACTGGGGGC TTCTAAACCA 
CCCCAAAAAT TACCAGAACT CAAACCTGGA CAGATAGTTG ATGAGAAGAA ACAGACCTCA 
GAGAAAGGTG TTCGTGTTGG AGAAAGAGAC GACACACTTC CACCACAATA CAGATTCAAA 
GAGGAAGATC TCAAGAAATA TCCTCCACCT GCGAAAGAGC CTTCACTGAA CACAGATGAT 
GCGATGGACA TACTTTCAGG AGATTTCACG ACCCCTGCAT CGGCACCTGT TGTCAAAGCT 
CCTGTTCCTC CTGCACAGGA CACAAAGAAG CATGCTGCAG TGCCTGAGAA GACCAAAGAT 
ATTCCCAAGA CTAAAGCTGA TGCTCTGTCA AGTGATTTCG TGGCTCCGGC ACATTCTGCT 
CCTAAGGTTT CTTCAGCTGC TCCTAAAACC ATCCCTCCAG GCCCACCACA AACCCCAGAT 
GAGGATCCTT TCAGTGCTTT GGGCGACACT CTTTCTGCCC CAGAACCACC AAAAAAACAA 
CCTGACCTGA AACCTAAGGA CATCGTACAT GAGAAGGATG TGACGTCAAA GAAAGGAGTT 
CGTGTTGGAG AAAGAGACGA CACACTCCCA CCAGAATACA GATTCAAAGA GGAAGATCTC 
AAGAAATATC CTCCTCCTCA GAAAGATCCT TCCTTAGATG CTAATGAAGC TCTGGATATT 
CTGTCTGGGG ATTTTACAAC TCCTTCCGCA CCATCGGCTG TTTCCCCTGC CTCTAAATCC 
TCTGCCAAGT CTTCAGATTC AGCTTTAGAT GCTCTTGCTG GTGATTTCGT CGCTTCTTCT 
TCTGCGTCTT CAGTTCATTC TGCCGTCTCT GGCCCCCCAC GCTCCGACAG ACTGTTGTCA 
GACGGAACCT CATCAGCTCT AGATGCCCTG TCAGAAACTC TGGGAGACAT TAAAGCAGCC 
CCTGAACCCG CCCCTGTCCT ACCTAAAACT GTCGTTAAGG AGAAGACGGT CATAGAAGAG 
AGAGTGAGTA AACCGGGTGA GAGAGACGAC TCCCTTCCAC CAGATCAGCG GTTCACTGCG 
GAGGATCGCA AGGCATTTGA AGAAGCAAAG AAGAAAGACG CCAAACCAAA ACAGCCATCA 
ATGGACGATT CAGCGGCTCT GGACATGTTG TCTAGTGATT TTTCAGATGT ACCGACTGTA 
AAGCCCACTG TATCTGATGG CAAAAGCTTC ACCCCTGAAC CAACACCACC AACCTTTAAG 
GCAACCGGTC CGGTTTTAGA CAAACTGGCA GACAACGTGC TTTCCAACCT GACTGACCCC 
AAAGCCAAAG ACAGCAAAGC AAAGGGAAAG GGGGCGAAAC CAAAGTCTAA ACCAAAGAAA 
CAGGCAGTGG AAGACTCTTC AGCCACAGAT CTGCTGTCCA GTAAACCGAG CTCAGATGTG 
GTGCCTTCAG CTTCCACAAA GGCTGGAAAA AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_005161034.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161091.1 Danio rerio calpastatin (cast), transcript variant X11, mRNA.

Price & Availability↑

CloneID	ODa25611
Clone ID Related Accession (Same CDS sequence)	XM_005161034.4 , XM_005161033.3 , XM_005161033.4 , XM_005161034.3
Accession Version	XM_005161034.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	calpastatin isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005161034.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC AGGTCACACC CACCACAACA TCTCAGGTCT CCACAGTGAA ACCTGCACAA 
TATGAGAAAG GAGCTACACA ATCGTCTACA GCAGCTGCGA CTGTCAAATC CGGCGCTGGA 
GATCCTGCAA CCACCCAGAA AGGATCTTCT CAAGTCAAAC CTCAGGTCAC AGATTCTAAA 
TCAACACCTG CATCTTCTGC TAAAGTCTCA GCTGTGAGCT CTGGGACTGG AGTTGCTGGT 
ACAACCGGTG GAGCAGGAGT GAAACCAGCA GATCCTGCAA AAGATACGCC TCAGAGCACC 
ACCATTCCTT CAACCAAACT GGGACCTGCT AAGGTGGATC CGGCTGCTGG GAAGCCCTCT 
GGCTCAGCTG GTGCCCAAAA ACAGACAAGT GCTCAAAAGG TGCAGGTTGA AGTGGGTCCT 
TCAGCAGCAA AAGTCAGTGC AGATGCTGAC GATCCGTTTG ATGCCCTGTC TGGCACATTA 
CCATCATCAC AACCTTTGGC TCCTAAAGTC CACTACACTG GACCTGAGAT CAAAGAGCCC 
AAACTGAAAT CAGAGAAAGG TGTTTTGTGT GGTGAGCGAG ATGACACGCT GCCGCCCGGA 
TACAGAAAGG AAGACCTGGA AAAGAAGCAT GGAGCTGGAG TTCCAGCTGG AGTTCCGGAG 
AAGCCTAAAG AAGTTCCCAA GCCCATAAGC ACAGATGATG CACTGGAATC TCTCTCCTCT 
ACGTTTGTGT CTCCAGCTCA AGCTGCTCCA AAGAAGACAG ACGTGAAAAC TGAAACAATA 
GGAGCAGTGG ATGTTAAATC GGCAGGCACC ACTAACTTTG CTCCCCCTCC ACCATCTCAG 
CAGAAACAAC CGGCGTCGTC TCAAGCTGCT GCTGTAACCA AATCACCTGC TCCACCGGCA 
GACAAGAAAG CTAAAATGGA GACTCATGCA CAACCAGCCA AAGCAAAGAC AGAGTCGGAC 
AGCTCCATAT CGCTGGACGC TCTCGATGCT TTGAGCGACA CACTGGGGGC TTCTAAACCA 
CCCCAAAAAT TACCAGAACT CAAACCTGGA CAGATAGTTG ATGAGAAGAA ACAGACCTCA 
GAGAAAGGTG TTCGTGTTGG AGAAAGAGAC GACACACTTC CACCACAATA CAGATTCAAA 
GAGGAAGATC TCAAGAAATA TCCTCCACCT GCGAAAGAGC CTTCACTGAA CACAGATGAT 
GCGATGGACA TACTTTCAGG AGATTTCACG ACCCCTGCAT CGGCACCTGT TGTCAAAGCT 
CCTGTTCCTC CTGCACAGGA CACAAAGAAG CATGCTGCAG TGCCTGAGAA GACCAAAGAT 
ATTCCCAAGA CTAAAGCTGA TGCTCTGTCA AGTGATTTCG TGGCTCCGGC ACATTCTGCT 
CCTAAGGTTT CTTCAGCTGC TCCTAAAACC ATCCCTCCAG GCCCACCACA AACCCCAGAT 
GAGGATCCTT TCAGTGCTTT GGGCGACACT CTTTCTGCCC CAGAACCACC AAAAAAACAA 
CCTGACCTGA AACCTAAGGA CATCGTACAT GAGAAGGATG TGACGTCAAA GAAAGGAGTT 
CGTGTTGGAG AAAGAGACGA CACACTCCCA CCAGAATACA GATTCAAAGA GGAAGATCTC 
AAGAAATATC CTCCTCCTCA GAAAGATCCT TCCTTAGATG CTAATGAAGC TCTGGATATT 
CTGTCTGGGG ATTTTACAAC TCCTTCCGCA CCATCGGCTG TTTCCCCTGC CTCTAAATCC 
TCTGCCAAGT CTTCAGATTC AGCTTTAGAT GCTCTTGCTG GTGATTTCGT CGCTTCTTCT 
TCTGCGTCTT CAGTTCATTC TGCCGTCTCT GGCCCCCCAC GCTCCGACAG ACTGTTGTCA 
GACGGAACCT CATCAGCTCT AGATGCCCTG TCAGAAACTC TGGGAGACAT TAAAGCAGCC 
CCTGAACCCG CCCCTGTCCT ACCTAAAACT GTCGTTAAGG AGAAGACGGT CATAGAAGAG 
AGAGTGAGTA AACCGGGTGA GAGAGACGAC TCCCTTCCAC CAGATCAGCG GTTCACTGCG 
GAGGATCGCA AGGCATTTGA AGAAGCAAAG AAGAAAGACG CCAAACCAAA ACAGCCATCA 
ATGGACGATT CAGCGGCTCT GGACATGTTG TCTAGTGATT TTTCAGATGT ACCGACTGTA 
AAGCCCACTG TATCTGATGG CAAAAGCTTC ACCCCTGAAC CAACACCACC AACCTTTAAG 
GCAACCGGTC CGGTTTTAGA CAAACTGGCA GACAACGTGC TTTCCAACCT GACTGACCCC 
AAAGCCAAAG ACAGCAAAGC AAAGGGAAAG GGGGCGAAAC CAAAGTCTAA ACCAAAGAAA 
CAGGCAGTGG AAGACTCTTC AGCCACAGAT CTGCTGTCCA GTAAACCGAG CTCAGATGTG 
GTGCCTTCAG CTTCCACAAA GGCTGGAAAA AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161091.1
CDS	230..2665
Translation	MSQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQVTDSKSTPASSAKVSAVSSGTGVAGTTGGAGVKPADPAKDTPQSTTIPSTKLGPAKVDPAAGKPSGSAGAQKQTSAQKVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_005161034.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161034.3

ORF Insert Sequence:

ATGTCTCAGC AGGTCACACC CACCACAACA TCTCAGGTCT CCACAGTGAA ACCTGCACAA 
TATGAGAAAG GAGCTACACA ATCGTCTACA GCAGCTGCGA CTGTCAAATC CGGCGCTGGA 
GATCCTGCAA CCACCCAGAA AGGATCTTCT CAAGTCAAAC CTCAGGTCAC AGATTCTAAA 
TCAACACCTG CATCTTCTGC TAAAGTCTCA GCTGTGAGCT CTGGGACTGG AGTTGCTGGT 
ACAACCGGTG GAGCAGGAGT GAAACCAGCA GATCCTGCAA AAGATACGCC TCAGAGCACC 
ACCATTCCTT CAACCAAACT GGGACCTGCT AAGGTGGATC CGGCTGCTGG GAAGCCCTCT 
GGCTCAGCTG GTGCCCAAAA ACAGACAAGT GCTCAAAAGG TGCAGGTTGA AGTGGGTCCT 
TCAGCAGCAA AAGTCAGTGC AGATGCTGAC GATCCGTTTG ATGCCCTGTC TGGCACATTA 
CCATCATCAC AACCTTTGGC TCCTAAAGTC CACTACACTG GACCTGAGAT CAAAGAGCCC 
AAACTGAAAT CAGAGAAAGG TGTTTTGTGT GGTGAGCGAG ATGACACGCT GCCGCCCGGA 
TACAGAAAGG AAGACCTGGA AAAGAAGCAT GGAGCTGGAG TTCCAGCTGG AGTTCCGGAG 
AAGCCTAAAG AAGTTCCCAA GCCCATAAGC ACAGATGATG CACTGGAATC TCTCTCCTCT 
ACGTTTGTGT CTCCAGCTCA AGCTGCTCCA AAGAAGACAG ACGTGAAAAC TGAAACAATA 
GGAGCAGTGG ATGTTAAATC GGCAGGCACC ACTAACTTTG CTCCCCCTCC ACCATCTCAG 
CAGAAACAAC CGGCGTCGTC TCAAGCTGCT GCTGTAACCA AATCACCTGC TCCACCGGCA 
GACAAGAAAG CTAAAATGGA GACTCATGCA CAACCAGCCA AAGCAAAGAC AGAGTCGGAC 
AGCTCCATAT CGCTGGACGC TCTCGATGCT TTGAGCGACA CACTGGGGGC TTCTAAACCA 
CCCCAAAAAT TACCAGAACT CAAACCTGGA CAGATAGTTG ATGAGAAGAA ACAGACCTCA 
GAGAAAGGTG TTCGTGTTGG AGAAAGAGAC GACACACTTC CACCACAATA CAGATTCAAA 
GAGGAAGATC TCAAGAAATA TCCTCCACCT GCGAAAGAGC CTTCACTGAA CACAGATGAT 
GCGATGGACA TACTTTCAGG AGATTTCACG ACCCCTGCAT CGGCACCTGT TGTCAAAGCT 
CCTGTTCCTC CTGCACAGGA CACAAAGAAG CATGCTGCAG TGCCTGAGAA GACCAAAGAT 
ATTCCCAAGA CTAAAGCTGA TGCTCTGTCA AGTGATTTCG TGGCTCCGGC ACATTCTGCT 
CCTAAGGTTT CTTCAGCTGC TCCTAAAACC ATCCCTCCAG GCCCACCACA AACCCCAGAT 
GAGGATCCTT TCAGTGCTTT GGGCGACACT CTTTCTGCCC CAGAACCACC AAAAAAACAA 
CCTGACCTGA AACCTAAGGA CATCGTACAT GAGAAGGATG TGACGTCAAA GAAAGGAGTT 
CGTGTTGGAG AAAGAGACGA CACACTCCCA CCAGAATACA GATTCAAAGA GGAAGATCTC 
AAGAAATATC CTCCTCCTCA GAAAGATCCT TCCTTAGATG CTAATGAAGC TCTGGATATT 
CTGTCTGGGG ATTTTACAAC TCCTTCCGCA CCATCGGCTG TTTCCCCTGC CTCTAAATCC 
TCTGCCAAGT CTTCAGATTC AGCTTTAGAT GCTCTTGCTG GTGATTTCGT CGCTTCTTCT 
TCTGCGTCTT CAGTTCATTC TGCCGTCTCT GGCCCCCCAC GCTCCGACAG ACTGTTGTCA 
GACGGAACCT CATCAGCTCT AGATGCCCTG TCAGAAACTC TGGGAGACAT TAAAGCAGCC 
CCTGAACCCG CCCCTGTCCT ACCTAAAACT GTCGTTAAGG AGAAGACGGT CATAGAAGAG 
AGAGTGAGTA AACCGGGTGA GAGAGACGAC TCCCTTCCAC CAGATCAGCG GTTCACTGCG 
GAGGATCGCA AGGCATTTGA AGAAGCAAAG AAGAAAGACG CCAAACCAAA ACAGCCATCA 
ATGGACGATT CAGCGGCTCT GGACATGTTG TCTAGTGATT TTTCAGATGT ACCGACTGTA 
AAGCCCACTG TATCTGATGG CAAAAGCTTC ACCCCTGAAC CAACACCACC AACCTTTAAG 
GCAACCGGTC CGGTTTTAGA CAAACTGGCA GACAACGTGC TTTCCAACCT GACTGACCCC 
AAAGCCAAAG ACAGCAAAGC AAAGGGAAAG GGGGCGAAAC CAAAGTCTAA ACCAAAGAAA 
CAGGCAGTGG AAGACTCTTC AGCCACAGAT CTGCTGTCCA GTAAACCGAG CTCAGATGTG 
GTGCCTTCAG CTTCCACAAA GGCTGGAAAA AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_005161036.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161093.1 Danio rerio calpastatin (cast), transcript variant X15, mRNA.

Price & Availability↑

CloneID	ODa25613
Clone ID Related Accession (Same CDS sequence)	XM_005161036.4 , XM_005161036.3
Accession Version	XM_005161036.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calpastatin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005161036.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCACA AAAAGAGATG TCATGGTCGT AAGAAAAACA AAAAGGAGAA TGGAGACACT 
TCATTTCAAA AACAAGAATA CGCTCCACAG ACATCAAGGT TCATGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGAA AGGAGCTACA 
CAATCGTCTA CAGCAGCTGC GACTGTCAAA TCCGGCGCTG GAGATCCTGC AACCACCCAG 
AAAGGATCTT CTCAAGTCAA ACCTCAGAGC ACCACCATTC CTTCAACCAA ACTGGGACCT 
GCTAAGGTGG ATCCGGCTGC TGGGAAGCCC TCTGGCTCAG CTGGTGCCCA AAAACAGACA 
AGTGCTCAAA AGGTGCAGGT TGAAGTGGGT CCTTCAGCAG CAAAAGTCAG TGCAGATGCT 
GACGATCCGT TTGATGCCCT GTCTGGCACA TTACCATCAT CACAACCTTT GGCTCCTAAA 
GTCCACTACA CTGGACCTGA GATCAAAGAG CCCAAACTGA AATCAGAGAA AGGTGTTTTG 
TGTGGTGAGC GAGATGACAC GCTGCCGCCC GGATACAGAA AGGAAGACCT GGAAAAGAAG 
CATGGAGCTG GAGTTCCAGC TGGAGTTCCG GAGAAGCCTA AAGAAGTTCC CAAGCCCATA 
AGCACAGATG ATGCACTGGA ATCTCTCTCC TCTACGTTTG TGTCTCCAGC TCAAGCTGCT 
CCAAAGAAGA CAGACGTGAA AACTGAAACA ATAGGAGCAG TGGATGTTAA ATCGGCAGGC 
ACCACTAACT TTGCTCCCCC TCCACCATCT CAGCAGAAAC AACCGGCGTC GTCTCAAGCT 
GCTGCTGTAA CCAAATCACC TGCTCCACCG GCAGACAAGA AAGCTAAAAT GGAGACTCAT 
GCACAACCAG CCAAAGCAAA GACAGAGTCG GACAGCTCCA TATCGCTGGA CGCTCTCGAT 
GCTTTGAGCG ACACACTGGG GGCTTCTAAA CCACCCCAAA AATTACCAGA ACTCAAACCT 
GGACAGATAG TTGATGAGAA GAAACAGACC TCAGAGAAAG GTGTTCGTGT TGGAGAAAGA 
GACGACACAC TTCCACCACA ATACAGATTC AAAGAGGAAG ATCTCAAGAA ATATCCTCCA 
CCTGCGAAAG AGCCTTCACT GAACACAGAT GATGCGATGG ACATACTTTC AGGAGATTTC 
ACGACCCCTG CATCGGCACC TGTTGTCAAA GCTCCTGTTC CTCCTGCACA GGACACAAAG 
AAGCATGCTG CAGTGCCTGA GAAGACCAAA GATATTCCCA AGACTAAAGC TGATGCTCTG 
TCAAGTGATT TCGTGGCTCC GGCACATTCT GCTCCTAAGG TTTCTTCAGC TGCTCCTAAA 
ACCATCCCTC CAGGCCCACC ACAAACCCCA GATGAGGATC CTTTCAGTGC TTTGGGCGAC 
ACTCTTTCTG CCCCAGAACC ACCAAAAAAA CAACCTGACC TGAAACCTAA GGACATCGTA 
CATGAGAAGG ATGTGACGTC AAAGAAAGGA GTTCGTGTTG GAGAAAGAGA CGACACACTC 
CCACCAGAAT ACAGATTCAA AGAGGAAGAT CTCAAGAAAT ATCCTCCTCC TCAGAAAGAT 
CCTTCCTTAG ATGCTAATGA AGCTCTGGAT ATTCTGTCTG GGGATTTTAC AACTCCTTCC 
GCACCATCGG CTGTTTCCCC TGCCTCTAAA TCCTCTGCCA AGTCTTCAGA TTCAGCTTTA 
GATGCTCTTG CTGGTGATTT CGTCGCTTCT TCTTCTGCGT CTTCAGTTCA TTCTGCCGTC 
TCTGGCCCCC CACGCTCCGA CAGACTGTTG TCAGACGGAA CCTCATCAGC TCTAGATGCC 
CTGTCAGAAA CTCTGGGAGA CATTAAAGCA GCCCCTGAAC CCGCCCCTGT CCTACCTAAA 
ACTGTCGTTA AGGAGAAGAC GGTCATAGAA GAGAGAGTGA GTAAACCGGG TGAGAGAGAC 
GACTCCCTTC CACCAGATCA GCGGTTCACT GCGGAGGATC GCAAGGCATT TGAAGAAGCA 
AAGAAGAAAG ACGCCAAACC AAAACAGCCA TCAATGGACG ATTCAGCGGC TCTGGACATG 
TTGTCTAGTG ATTTTTCAGA TGTACCGACT GTAAAGCCCA CTGTATCTGA TGGCAAAAGC 
TTCACCCCTG AACCAACACC ACCAACCTTT AAGGCAACCG GTCCGGTTTT AGACAAACTG 
GCAGACAACG TGCTTTCCAA CCTGACTGAC CCCAAAGCCA AAGACAGCAA AGCAAAGGGA 
AAGGGGGCGA AACCAAAGTC TAAACCAAAG AAACAGGCAG TGGAAGACTC TTCAGCCACA 
GATCTGCTGT CCAGTAAACC GAGCTCAGAT GTGGTGCCTT CAGCTTCCAC AAAGGCTGGA 
AAAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161093.1
CDS	29..2437
Translation	MPHKKRCHGRKKNKKENGDTSFQKQEYAPQTSRFMSQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQSTTIPSTKLGPAKVDPAAGKPSGSAGAQKQTSAQKVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_005161036.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161036.4

ORF Insert Sequence:

ATGCCGCACA AAAAGAGATG TCATGGTCGT AAGAAAAACA AAAAGGAGAA TGGAGACACT 
TCATTTCAAA AACAAGAATA CGCTCCACAG ACATCAAGGT TCATGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGAA AGGAGCTACA 
CAATCGTCTA CAGCAGCTGC GACTGTCAAA TCCGGCGCTG GAGATCCTGC AACCACCCAG 
AAAGGATCTT CTCAAGTCAA ACCTCAGAGC ACCACCATTC CTTCAACCAA ACTGGGACCT 
GCTAAGGTGG ATCCGGCTGC TGGGAAGCCC TCTGGCTCAG CTGGTGCCCA AAAACAGACA 
AGTGCTCAAA AGGTGCAGGT TGAAGTGGGT CCTTCAGCAG CAAAAGTCAG TGCAGATGCT 
GACGATCCGT TTGATGCCCT GTCTGGCACA TTACCATCAT CACAACCTTT GGCTCCTAAA 
GTCCACTACA CTGGACCTGA GATCAAAGAG CCCAAACTGA AATCAGAGAA AGGTGTTTTG 
TGTGGTGAGC GAGATGACAC GCTGCCGCCC GGATACAGAA AGGAAGACCT GGAAAAGAAG 
CATGGAGCTG GAGTTCCAGC TGGAGTTCCG GAGAAGCCTA AAGAAGTTCC CAAGCCCATA 
AGCACAGATG ATGCACTGGA ATCTCTCTCC TCTACGTTTG TGTCTCCAGC TCAAGCTGCT 
CCAAAGAAGA CAGACGTGAA AACTGAAACA ATAGGAGCAG TGGATGTTAA ATCGGCAGGC 
ACCACTAACT TTGCTCCCCC TCCACCATCT CAGCAGAAAC AACCGGCGTC GTCTCAAGCT 
GCTGCTGTAA CCAAATCACC TGCTCCACCG GCAGACAAGA AAGCTAAAAT GGAGACTCAT 
GCACAACCAG CCAAAGCAAA GACAGAGTCG GACAGCTCCA TATCGCTGGA CGCTCTCGAT 
GCTTTGAGCG ACACACTGGG GGCTTCTAAA CCACCCCAAA AATTACCAGA ACTCAAACCT 
GGACAGATAG TTGATGAGAA GAAACAGACC TCAGAGAAAG GTGTTCGTGT TGGAGAAAGA 
GACGACACAC TTCCACCACA ATACAGATTC AAAGAGGAAG ATCTCAAGAA ATATCCTCCA 
CCTGCGAAAG AGCCTTCACT GAACACAGAT GATGCGATGG ACATACTTTC AGGAGATTTC 
ACGACCCCTG CATCGGCACC TGTTGTCAAA GCTCCTGTTC CTCCTGCACA GGACACAAAG 
AAGCATGCTG CAGTGCCTGA GAAGACCAAA GATATTCCCA AGACTAAAGC TGATGCTCTG 
TCAAGTGATT TCGTGGCTCC GGCACATTCT GCTCCTAAGG TTTCTTCAGC TGCTCCTAAA 
ACCATCCCTC CAGGCCCACC ACAAACCCCA GATGAGGATC CTTTCAGTGC TTTGGGCGAC 
ACTCTTTCTG CCCCAGAACC ACCAAAAAAA CAACCTGACC TGAAACCTAA GGACATCGTA 
CATGAGAAGG ATGTGACGTC AAAGAAAGGA GTTCGTGTTG GAGAAAGAGA CGACACACTC 
CCACCAGAAT ACAGATTCAA AGAGGAAGAT CTCAAGAAAT ATCCTCCTCC TCAGAAAGAT 
CCTTCCTTAG ATGCTAATGA AGCTCTGGAT ATTCTGTCTG GGGATTTTAC AACTCCTTCC 
GCACCATCGG CTGTTTCCCC TGCCTCTAAA TCCTCTGCCA AGTCTTCAGA TTCAGCTTTA 
GATGCTCTTG CTGGTGATTT CGTCGCTTCT TCTTCTGCGT CTTCAGTTCA TTCTGCCGTC 
TCTGGCCCCC CACGCTCCGA CAGACTGTTG TCAGACGGAA CCTCATCAGC TCTAGATGCC 
CTGTCAGAAA CTCTGGGAGA CATTAAAGCA GCCCCTGAAC CCGCCCCTGT CCTACCTAAA 
ACTGTCGTTA AGGAGAAGAC GGTCATAGAA GAGAGAGTGA GTAAACCGGG TGAGAGAGAC 
GACTCCCTTC CACCAGATCA GCGGTTCACT GCGGAGGATC GCAAGGCATT TGAAGAAGCA 
AAGAAGAAAG ACGCCAAACC AAAACAGCCA TCAATGGACG ATTCAGCGGC TCTGGACATG 
TTGTCTAGTG ATTTTTCAGA TGTACCGACT GTAAAGCCCA CTGTATCTGA TGGCAAAAGC 
TTCACCCCTG AACCAACACC ACCAACCTTT AAGGCAACCG GTCCGGTTTT AGACAAACTG 
GCAGACAACG TGCTTTCCAA CCTGACTGAC CCCAAAGCCA AAGACAGCAA AGCAAAGGGA 
AAGGGGGCGA AACCAAAGTC TAAACCAAAG AAACAGGCAG TGGAAGACTC TTCAGCCACA 
GATCTGCTGT CCAGTAAACC GAGCTCAGAT GTGGTGCCTT CAGCTTCCAC AAAGGCTGGA 
AAAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_005161036.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161093.1 Danio rerio calpastatin (cast), transcript variant X14, mRNA.

Price & Availability↑

CloneID	ODa25613
Clone ID Related Accession (Same CDS sequence)	XM_005161036.4 , XM_005161036.3
Accession Version	XM_005161036.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	calpastatin isoform X14
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005161036.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCACA AAAAGAGATG TCATGGTCGT AAGAAAAACA AAAAGGAGAA TGGAGACACT 
TCATTTCAAA AACAAGAATA CGCTCCACAG ACATCAAGGT TCATGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGAA AGGAGCTACA 
CAATCGTCTA CAGCAGCTGC GACTGTCAAA TCCGGCGCTG GAGATCCTGC AACCACCCAG 
AAAGGATCTT CTCAAGTCAA ACCTCAGAGC ACCACCATTC CTTCAACCAA ACTGGGACCT 
GCTAAGGTGG ATCCGGCTGC TGGGAAGCCC TCTGGCTCAG CTGGTGCCCA AAAACAGACA 
AGTGCTCAAA AGGTGCAGGT TGAAGTGGGT CCTTCAGCAG CAAAAGTCAG TGCAGATGCT 
GACGATCCGT TTGATGCCCT GTCTGGCACA TTACCATCAT CACAACCTTT GGCTCCTAAA 
GTCCACTACA CTGGACCTGA GATCAAAGAG CCCAAACTGA AATCAGAGAA AGGTGTTTTG 
TGTGGTGAGC GAGATGACAC GCTGCCGCCC GGATACAGAA AGGAAGACCT GGAAAAGAAG 
CATGGAGCTG GAGTTCCAGC TGGAGTTCCG GAGAAGCCTA AAGAAGTTCC CAAGCCCATA 
AGCACAGATG ATGCACTGGA ATCTCTCTCC TCTACGTTTG TGTCTCCAGC TCAAGCTGCT 
CCAAAGAAGA CAGACGTGAA AACTGAAACA ATAGGAGCAG TGGATGTTAA ATCGGCAGGC 
ACCACTAACT TTGCTCCCCC TCCACCATCT CAGCAGAAAC AACCGGCGTC GTCTCAAGCT 
GCTGCTGTAA CCAAATCACC TGCTCCACCG GCAGACAAGA AAGCTAAAAT GGAGACTCAT 
GCACAACCAG CCAAAGCAAA GACAGAGTCG GACAGCTCCA TATCGCTGGA CGCTCTCGAT 
GCTTTGAGCG ACACACTGGG GGCTTCTAAA CCACCCCAAA AATTACCAGA ACTCAAACCT 
GGACAGATAG TTGATGAGAA GAAACAGACC TCAGAGAAAG GTGTTCGTGT TGGAGAAAGA 
GACGACACAC TTCCACCACA ATACAGATTC AAAGAGGAAG ATCTCAAGAA ATATCCTCCA 
CCTGCGAAAG AGCCTTCACT GAACACAGAT GATGCGATGG ACATACTTTC AGGAGATTTC 
ACGACCCCTG CATCGGCACC TGTTGTCAAA GCTCCTGTTC CTCCTGCACA GGACACAAAG 
AAGCATGCTG CAGTGCCTGA GAAGACCAAA GATATTCCCA AGACTAAAGC TGATGCTCTG 
TCAAGTGATT TCGTGGCTCC GGCACATTCT GCTCCTAAGG TTTCTTCAGC TGCTCCTAAA 
ACCATCCCTC CAGGCCCACC ACAAACCCCA GATGAGGATC CTTTCAGTGC TTTGGGCGAC 
ACTCTTTCTG CCCCAGAACC ACCAAAAAAA CAACCTGACC TGAAACCTAA GGACATCGTA 
CATGAGAAGG ATGTGACGTC AAAGAAAGGA GTTCGTGTTG GAGAAAGAGA CGACACACTC 
CCACCAGAAT ACAGATTCAA AGAGGAAGAT CTCAAGAAAT ATCCTCCTCC TCAGAAAGAT 
CCTTCCTTAG ATGCTAATGA AGCTCTGGAT ATTCTGTCTG GGGATTTTAC AACTCCTTCC 
GCACCATCGG CTGTTTCCCC TGCCTCTAAA TCCTCTGCCA AGTCTTCAGA TTCAGCTTTA 
GATGCTCTTG CTGGTGATTT CGTCGCTTCT TCTTCTGCGT CTTCAGTTCA TTCTGCCGTC 
TCTGGCCCCC CACGCTCCGA CAGACTGTTG TCAGACGGAA CCTCATCAGC TCTAGATGCC 
CTGTCAGAAA CTCTGGGAGA CATTAAAGCA GCCCCTGAAC CCGCCCCTGT CCTACCTAAA 
ACTGTCGTTA AGGAGAAGAC GGTCATAGAA GAGAGAGTGA GTAAACCGGG TGAGAGAGAC 
GACTCCCTTC CACCAGATCA GCGGTTCACT GCGGAGGATC GCAAGGCATT TGAAGAAGCA 
AAGAAGAAAG ACGCCAAACC AAAACAGCCA TCAATGGACG ATTCAGCGGC TCTGGACATG 
TTGTCTAGTG ATTTTTCAGA TGTACCGACT GTAAAGCCCA CTGTATCTGA TGGCAAAAGC 
TTCACCCCTG AACCAACACC ACCAACCTTT AAGGCAACCG GTCCGGTTTT AGACAAACTG 
GCAGACAACG TGCTTTCCAA CCTGACTGAC CCCAAAGCCA AAGACAGCAA AGCAAAGGGA 
AAGGGGGCGA AACCAAAGTC TAAACCAAAG AAACAGGCAG TGGAAGACTC TTCAGCCACA 
GATCTGCTGT CCAGTAAACC GAGCTCAGAT GTGGTGCCTT CAGCTTCCAC AAAGGCTGGA 
AAAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161093.1
CDS	40..2448
Translation	MPHKKRCHGRKKNKKENGDTSFQKQEYAPQTSRFMSQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQSTTIPSTKLGPAKVDPAAGKPSGSAGAQKQTSAQKVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_005161036.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161036.3

ORF Insert Sequence:

ATGCCGCACA AAAAGAGATG TCATGGTCGT AAGAAAAACA AAAAGGAGAA TGGAGACACT 
TCATTTCAAA AACAAGAATA CGCTCCACAG ACATCAAGGT TCATGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGAA AGGAGCTACA 
CAATCGTCTA CAGCAGCTGC GACTGTCAAA TCCGGCGCTG GAGATCCTGC AACCACCCAG 
AAAGGATCTT CTCAAGTCAA ACCTCAGAGC ACCACCATTC CTTCAACCAA ACTGGGACCT 
GCTAAGGTGG ATCCGGCTGC TGGGAAGCCC TCTGGCTCAG CTGGTGCCCA AAAACAGACA 
AGTGCTCAAA AGGTGCAGGT TGAAGTGGGT CCTTCAGCAG CAAAAGTCAG TGCAGATGCT 
GACGATCCGT TTGATGCCCT GTCTGGCACA TTACCATCAT CACAACCTTT GGCTCCTAAA 
GTCCACTACA CTGGACCTGA GATCAAAGAG CCCAAACTGA AATCAGAGAA AGGTGTTTTG 
TGTGGTGAGC GAGATGACAC GCTGCCGCCC GGATACAGAA AGGAAGACCT GGAAAAGAAG 
CATGGAGCTG GAGTTCCAGC TGGAGTTCCG GAGAAGCCTA AAGAAGTTCC CAAGCCCATA 
AGCACAGATG ATGCACTGGA ATCTCTCTCC TCTACGTTTG TGTCTCCAGC TCAAGCTGCT 
CCAAAGAAGA CAGACGTGAA AACTGAAACA ATAGGAGCAG TGGATGTTAA ATCGGCAGGC 
ACCACTAACT TTGCTCCCCC TCCACCATCT CAGCAGAAAC AACCGGCGTC GTCTCAAGCT 
GCTGCTGTAA CCAAATCACC TGCTCCACCG GCAGACAAGA AAGCTAAAAT GGAGACTCAT 
GCACAACCAG CCAAAGCAAA GACAGAGTCG GACAGCTCCA TATCGCTGGA CGCTCTCGAT 
GCTTTGAGCG ACACACTGGG GGCTTCTAAA CCACCCCAAA AATTACCAGA ACTCAAACCT 
GGACAGATAG TTGATGAGAA GAAACAGACC TCAGAGAAAG GTGTTCGTGT TGGAGAAAGA 
GACGACACAC TTCCACCACA ATACAGATTC AAAGAGGAAG ATCTCAAGAA ATATCCTCCA 
CCTGCGAAAG AGCCTTCACT GAACACAGAT GATGCGATGG ACATACTTTC AGGAGATTTC 
ACGACCCCTG CATCGGCACC TGTTGTCAAA GCTCCTGTTC CTCCTGCACA GGACACAAAG 
AAGCATGCTG CAGTGCCTGA GAAGACCAAA GATATTCCCA AGACTAAAGC TGATGCTCTG 
TCAAGTGATT TCGTGGCTCC GGCACATTCT GCTCCTAAGG TTTCTTCAGC TGCTCCTAAA 
ACCATCCCTC CAGGCCCACC ACAAACCCCA GATGAGGATC CTTTCAGTGC TTTGGGCGAC 
ACTCTTTCTG CCCCAGAACC ACCAAAAAAA CAACCTGACC TGAAACCTAA GGACATCGTA 
CATGAGAAGG ATGTGACGTC AAAGAAAGGA GTTCGTGTTG GAGAAAGAGA CGACACACTC 
CCACCAGAAT ACAGATTCAA AGAGGAAGAT CTCAAGAAAT ATCCTCCTCC TCAGAAAGAT 
CCTTCCTTAG ATGCTAATGA AGCTCTGGAT ATTCTGTCTG GGGATTTTAC AACTCCTTCC 
GCACCATCGG CTGTTTCCCC TGCCTCTAAA TCCTCTGCCA AGTCTTCAGA TTCAGCTTTA 
GATGCTCTTG CTGGTGATTT CGTCGCTTCT TCTTCTGCGT CTTCAGTTCA TTCTGCCGTC 
TCTGGCCCCC CACGCTCCGA CAGACTGTTG TCAGACGGAA CCTCATCAGC TCTAGATGCC 
CTGTCAGAAA CTCTGGGAGA CATTAAAGCA GCCCCTGAAC CCGCCCCTGT CCTACCTAAA 
ACTGTCGTTA AGGAGAAGAC GGTCATAGAA GAGAGAGTGA GTAAACCGGG TGAGAGAGAC 
GACTCCCTTC CACCAGATCA GCGGTTCACT GCGGAGGATC GCAAGGCATT TGAAGAAGCA 
AAGAAGAAAG ACGCCAAACC AAAACAGCCA TCAATGGACG ATTCAGCGGC TCTGGACATG 
TTGTCTAGTG ATTTTTCAGA TGTACCGACT GTAAAGCCCA CTGTATCTGA TGGCAAAAGC 
TTCACCCCTG AACCAACACC ACCAACCTTT AAGGCAACCG GTCCGGTTTT AGACAAACTG 
GCAGACAACG TGCTTTCCAA CCTGACTGAC CCCAAAGCCA AAGACAGCAA AGCAAAGGGA 
AAGGGGGCGA AACCAAAGTC TAAACCAAAG AAACAGGCAG TGGAAGACTC TTCAGCCACA 
GATCTGCTGT CCAGTAAACC GAGCTCAGAT GTGGTGCCTT CAGCTTCCAC AAAGGCTGGA 
AAAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_005161037.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161094.1 Danio rerio calpastatin (cast), transcript variant X15, mRNA.

Price & Availability↑

CloneID	ODa25614
Clone ID Related Accession (Same CDS sequence)	XM_005161037.3 , XM_005161037.4
Accession Version	XM_005161037.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	calpastatin isoform X15
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005161037.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGTCTCA GCAGGTCACA CCCACCACAA CATCTCAGGT CTCCACAGTG 
AAACCTGCAC AATATGAGAA AGGAGCTACA CAATCGTCTA CAGCAGCTGC GACTGTCAAA 
TCCGGCGCTG GAGATCCTGC AACCACCCAG AAAGGATCTT CTCAAGTCAA ACCTCAGAGC 
ACCACCATTC CTTCAACCAA ACTGGGACCT GCTAAGGTGG ATCCGGCTGC TGGGAAGCCC 
TCTGGCTCAG CTGGTGCCCA AAAACAGACA AGTGCTCAAA AGGTGCAGGT TGAAGTGGGT 
CCTTCAGCAG CAAAAGTCAG TGCAGATGCT GACGATCCGT TTGATGCCCT GTCTGGCACA 
TTACCATCAT CACAACCTTT GGCTCCTAAA GTCCACTACA CTGGACCTGA GATCAAAGAG 
CCCAAACTGA AATCAGAGAA AGGTGTTTTG TGTGGTGAGC GAGATGACAC GCTGCCGCCC 
GGATACAGAA AGGAAGACCT GGAAAAGAAG CATGGAGCTG GAGTTCCAGC TGGAGTTCCG 
GAGAAGCCTA AAGAAGTTCC CAAGCCCATA AGCACAGATG ATGCACTGGA ATCTCTCTCC 
TCTACGTTTG TGTCTCCAGC TCAAGCTGCT CCAAAGAAGA CAGACGTGAA AACTGAAACA 
ATAGGAGCAG TGGATGTTAA ATCGGCAGGC ACCACTAACT TTGCTCCCCC TCCACCATCT 
CAGCAGAAAC AACCGGCGTC GTCTCAAGCT GCTGCTGTAA CCAAATCACC TGCTCCACCG 
GCAGACAAGA AAGCTAAAAT GGAGACTCAT GCACAACCAG CCAAAGCAAA GACAGAGTCG 
GACAGCTCCA TATCGCTGGA CGCTCTCGAT GCTTTGAGCG ACACACTGGG GGCTTCTAAA 
CCACCCCAAA AATTACCAGA ACTCAAACCT GGACAGATAG TTGATGAGAA GAAACAGACC 
TCAGAGAAAG GTGTTCGTGT TGGAGAAAGA GACGACACAC TTCCACCACA ATACAGATTC 
AAAGAGGAAG ATCTCAAGAA ATATCCTCCA CCTGCGAAAG AGCCTTCACT GAACACAGAT 
GATGCGATGG ACATACTTTC AGGAGATTTC ACGACCCCTG CATCGGCACC TGTTGTCAAA 
GCTCCTGTTC CTCCTGCACA GGACACAAAG AAGCATGCTG CAGTGCCTGA GAAGACCAAA 
GATATTCCCA AGACTAAAGC TGATGCTCTG TCAAGTGATT TCGTGGCTCC GGCACATTCT 
GCTCCTAAGG TTTCTTCAGC TGCTCCTAAA ACCATCCCTC CAGGCCCACC ACAAACCCCA 
GATGAGGATC CTTTCAGTGC TTTGGGCGAC ACTCTTTCTG CCCCAGAACC ACCAAAAAAA 
CAACCTGACC TGAAACCTAA GGACATCGTA CATGAGAAGG ATGTGACGTC AAAGAAAGGA 
GTTCGTGTTG GAGAAAGAGA CGACACACTC CCACCAGAAT ACAGATTCAA AGAGGAAGAT 
CTCAAGAAAT ATCCTCCTCC TCAGAAAGAT CCTTCCTTAG ATGCTAATGA AGCTCTGGAT 
ATTCTGTCTG GGGATTTTAC AACTCCTTCC GCACCATCGG CTGTTTCCCC TGCCTCTAAA 
TCCTCTGCCA AGTCTTCAGA TTCAGCTTTA GATGCTCTTG CTGGTGATTT CGTCGCTTCT 
TCTTCTGCGT CTTCAGTTCA TTCTGCCGTC TCTGGCCCCC CACGCTCCGA CAGACTGTTG 
TCAGACGGAA CCTCATCAGC TCTAGATGCC CTGTCAGAAA CTCTGGGAGA CATTAAAGCA 
GCCCCTGAAC CCGCCCCTGT CCTACCTAAA ACTGTCGTTA AGGAGAAGAC GGTCATAGAA 
GAGAGAGTGA GTAAACCGGG TGAGAGAGAC GACTCCCTTC CACCAGATCA GCGGTTCACT 
GCGGAGGATC GCAAGGCATT TGAAGAAGCA AAGAAGAAAG ACGCCAAACC AAAACAGCCA 
TCAATGGACG ATTCAGCGGC TCTGGACATG TTGTCTAGTG ATTTTTCAGA TGTACCGACT 
GTAAAGCCCA CTGTATCTGA TGGCAAAAGC TTCACCCCTG AACCAACACC ACCAACCTTT 
AAGGCAACCG GTCCGGTTTT AGACAAACTG GCAGACAACG TGCTTTCCAA CCTGACTGAC 
CCCAAAGCCA AAGACAGCAA AGCAAAGGGA AAGGGGGCGA AACCAAAGTC TAAACCAAAG 
AAACAGGCAG TGGAAGACTC TTCAGCCACA GATCTGCTGT CCAGTAAACC GAGCTCAGAT 
GTGGTGCCTT CAGCTTCCAC AAAGGCTGGA AAAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161094.1
CDS	68..2446
Translation	MGQIFSWIRGTRDQPALQDIAVEQQSQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQSTTIPSTKLGPAKVDPAAGKPSGSAGAQKQTSAQKVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_005161037.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161037.3

ORF Insert Sequence:

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGTCTCA GCAGGTCACA CCCACCACAA CATCTCAGGT CTCCACAGTG 
AAACCTGCAC AATATGAGAA AGGAGCTACA CAATCGTCTA CAGCAGCTGC GACTGTCAAA 
TCCGGCGCTG GAGATCCTGC AACCACCCAG AAAGGATCTT CTCAAGTCAA ACCTCAGAGC 
ACCACCATTC CTTCAACCAA ACTGGGACCT GCTAAGGTGG ATCCGGCTGC TGGGAAGCCC 
TCTGGCTCAG CTGGTGCCCA AAAACAGACA AGTGCTCAAA AGGTGCAGGT TGAAGTGGGT 
CCTTCAGCAG CAAAAGTCAG TGCAGATGCT GACGATCCGT TTGATGCCCT GTCTGGCACA 
TTACCATCAT CACAACCTTT GGCTCCTAAA GTCCACTACA CTGGACCTGA GATCAAAGAG 
CCCAAACTGA AATCAGAGAA AGGTGTTTTG TGTGGTGAGC GAGATGACAC GCTGCCGCCC 
GGATACAGAA AGGAAGACCT GGAAAAGAAG CATGGAGCTG GAGTTCCAGC TGGAGTTCCG 
GAGAAGCCTA AAGAAGTTCC CAAGCCCATA AGCACAGATG ATGCACTGGA ATCTCTCTCC 
TCTACGTTTG TGTCTCCAGC TCAAGCTGCT CCAAAGAAGA CAGACGTGAA AACTGAAACA 
ATAGGAGCAG TGGATGTTAA ATCGGCAGGC ACCACTAACT TTGCTCCCCC TCCACCATCT 
CAGCAGAAAC AACCGGCGTC GTCTCAAGCT GCTGCTGTAA CCAAATCACC TGCTCCACCG 
GCAGACAAGA AAGCTAAAAT GGAGACTCAT GCACAACCAG CCAAAGCAAA GACAGAGTCG 
GACAGCTCCA TATCGCTGGA CGCTCTCGAT GCTTTGAGCG ACACACTGGG GGCTTCTAAA 
CCACCCCAAA AATTACCAGA ACTCAAACCT GGACAGATAG TTGATGAGAA GAAACAGACC 
TCAGAGAAAG GTGTTCGTGT TGGAGAAAGA GACGACACAC TTCCACCACA ATACAGATTC 
AAAGAGGAAG ATCTCAAGAA ATATCCTCCA CCTGCGAAAG AGCCTTCACT GAACACAGAT 
GATGCGATGG ACATACTTTC AGGAGATTTC ACGACCCCTG CATCGGCACC TGTTGTCAAA 
GCTCCTGTTC CTCCTGCACA GGACACAAAG AAGCATGCTG CAGTGCCTGA GAAGACCAAA 
GATATTCCCA AGACTAAAGC TGATGCTCTG TCAAGTGATT TCGTGGCTCC GGCACATTCT 
GCTCCTAAGG TTTCTTCAGC TGCTCCTAAA ACCATCCCTC CAGGCCCACC ACAAACCCCA 
GATGAGGATC CTTTCAGTGC TTTGGGCGAC ACTCTTTCTG CCCCAGAACC ACCAAAAAAA 
CAACCTGACC TGAAACCTAA GGACATCGTA CATGAGAAGG ATGTGACGTC AAAGAAAGGA 
GTTCGTGTTG GAGAAAGAGA CGACACACTC CCACCAGAAT ACAGATTCAA AGAGGAAGAT 
CTCAAGAAAT ATCCTCCTCC TCAGAAAGAT CCTTCCTTAG ATGCTAATGA AGCTCTGGAT 
ATTCTGTCTG GGGATTTTAC AACTCCTTCC GCACCATCGG CTGTTTCCCC TGCCTCTAAA 
TCCTCTGCCA AGTCTTCAGA TTCAGCTTTA GATGCTCTTG CTGGTGATTT CGTCGCTTCT 
TCTTCTGCGT CTTCAGTTCA TTCTGCCGTC TCTGGCCCCC CACGCTCCGA CAGACTGTTG 
TCAGACGGAA CCTCATCAGC TCTAGATGCC CTGTCAGAAA CTCTGGGAGA CATTAAAGCA 
GCCCCTGAAC CCGCCCCTGT CCTACCTAAA ACTGTCGTTA AGGAGAAGAC GGTCATAGAA 
GAGAGAGTGA GTAAACCGGG TGAGAGAGAC GACTCCCTTC CACCAGATCA GCGGTTCACT 
GCGGAGGATC GCAAGGCATT TGAAGAAGCA AAGAAGAAAG ACGCCAAACC AAAACAGCCA 
TCAATGGACG ATTCAGCGGC TCTGGACATG TTGTCTAGTG ATTTTTCAGA TGTACCGACT 
GTAAAGCCCA CTGTATCTGA TGGCAAAAGC TTCACCCCTG AACCAACACC ACCAACCTTT 
AAGGCAACCG GTCCGGTTTT AGACAAACTG GCAGACAACG TGCTTTCCAA CCTGACTGAC 
CCCAAAGCCA AAGACAGCAA AGCAAAGGGA AAGGGGGCGA AACCAAAGTC TAAACCAAAG 
AAACAGGCAG TGGAAGACTC TTCAGCCACA GATCTGCTGT CCAGTAAACC GAGCTCAGAT 
GTGGTGCCTT CAGCTTCCAC AAAGGCTGGA AAAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_005161037.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161094.1 Danio rerio calpastatin (cast), transcript variant X16, mRNA.

Price & Availability↑

CloneID	ODa25614
Clone ID Related Accession (Same CDS sequence)	XM_005161037.3 , XM_005161037.4
Accession Version	XM_005161037.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calpastatin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005161037.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGTCTCA GCAGGTCACA CCCACCACAA CATCTCAGGT CTCCACAGTG 
AAACCTGCAC AATATGAGAA AGGAGCTACA CAATCGTCTA CAGCAGCTGC GACTGTCAAA 
TCCGGCGCTG GAGATCCTGC AACCACCCAG AAAGGATCTT CTCAAGTCAA ACCTCAGAGC 
ACCACCATTC CTTCAACCAA ACTGGGACCT GCTAAGGTGG ATCCGGCTGC TGGGAAGCCC 
TCTGGCTCAG CTGGTGCCCA AAAACAGACA AGTGCTCAAA AGGTGCAGGT TGAAGTGGGT 
CCTTCAGCAG CAAAAGTCAG TGCAGATGCT GACGATCCGT TTGATGCCCT GTCTGGCACA 
TTACCATCAT CACAACCTTT GGCTCCTAAA GTCCACTACA CTGGACCTGA GATCAAAGAG 
CCCAAACTGA AATCAGAGAA AGGTGTTTTG TGTGGTGAGC GAGATGACAC GCTGCCGCCC 
GGATACAGAA AGGAAGACCT GGAAAAGAAG CATGGAGCTG GAGTTCCAGC TGGAGTTCCG 
GAGAAGCCTA AAGAAGTTCC CAAGCCCATA AGCACAGATG ATGCACTGGA ATCTCTCTCC 
TCTACGTTTG TGTCTCCAGC TCAAGCTGCT CCAAAGAAGA CAGACGTGAA AACTGAAACA 
ATAGGAGCAG TGGATGTTAA ATCGGCAGGC ACCACTAACT TTGCTCCCCC TCCACCATCT 
CAGCAGAAAC AACCGGCGTC GTCTCAAGCT GCTGCTGTAA CCAAATCACC TGCTCCACCG 
GCAGACAAGA AAGCTAAAAT GGAGACTCAT GCACAACCAG CCAAAGCAAA GACAGAGTCG 
GACAGCTCCA TATCGCTGGA CGCTCTCGAT GCTTTGAGCG ACACACTGGG GGCTTCTAAA 
CCACCCCAAA AATTACCAGA ACTCAAACCT GGACAGATAG TTGATGAGAA GAAACAGACC 
TCAGAGAAAG GTGTTCGTGT TGGAGAAAGA GACGACACAC TTCCACCACA ATACAGATTC 
AAAGAGGAAG ATCTCAAGAA ATATCCTCCA CCTGCGAAAG AGCCTTCACT GAACACAGAT 
GATGCGATGG ACATACTTTC AGGAGATTTC ACGACCCCTG CATCGGCACC TGTTGTCAAA 
GCTCCTGTTC CTCCTGCACA GGACACAAAG AAGCATGCTG CAGTGCCTGA GAAGACCAAA 
GATATTCCCA AGACTAAAGC TGATGCTCTG TCAAGTGATT TCGTGGCTCC GGCACATTCT 
GCTCCTAAGG TTTCTTCAGC TGCTCCTAAA ACCATCCCTC CAGGCCCACC ACAAACCCCA 
GATGAGGATC CTTTCAGTGC TTTGGGCGAC ACTCTTTCTG CCCCAGAACC ACCAAAAAAA 
CAACCTGACC TGAAACCTAA GGACATCGTA CATGAGAAGG ATGTGACGTC AAAGAAAGGA 
GTTCGTGTTG GAGAAAGAGA CGACACACTC CCACCAGAAT ACAGATTCAA AGAGGAAGAT 
CTCAAGAAAT ATCCTCCTCC TCAGAAAGAT CCTTCCTTAG ATGCTAATGA AGCTCTGGAT 
ATTCTGTCTG GGGATTTTAC AACTCCTTCC GCACCATCGG CTGTTTCCCC TGCCTCTAAA 
TCCTCTGCCA AGTCTTCAGA TTCAGCTTTA GATGCTCTTG CTGGTGATTT CGTCGCTTCT 
TCTTCTGCGT CTTCAGTTCA TTCTGCCGTC TCTGGCCCCC CACGCTCCGA CAGACTGTTG 
TCAGACGGAA CCTCATCAGC TCTAGATGCC CTGTCAGAAA CTCTGGGAGA CATTAAAGCA 
GCCCCTGAAC CCGCCCCTGT CCTACCTAAA ACTGTCGTTA AGGAGAAGAC GGTCATAGAA 
GAGAGAGTGA GTAAACCGGG TGAGAGAGAC GACTCCCTTC CACCAGATCA GCGGTTCACT 
GCGGAGGATC GCAAGGCATT TGAAGAAGCA AAGAAGAAAG ACGCCAAACC AAAACAGCCA 
TCAATGGACG ATTCAGCGGC TCTGGACATG TTGTCTAGTG ATTTTTCAGA TGTACCGACT 
GTAAAGCCCA CTGTATCTGA TGGCAAAAGC TTCACCCCTG AACCAACACC ACCAACCTTT 
AAGGCAACCG GTCCGGTTTT AGACAAACTG GCAGACAACG TGCTTTCCAA CCTGACTGAC 
CCCAAAGCCA AAGACAGCAA AGCAAAGGGA AAGGGGGCGA AACCAAAGTC TAAACCAAAG 
AAACAGGCAG TGGAAGACTC TTCAGCCACA GATCTGCTGT CCAGTAAACC GAGCTCAGAT 
GTGGTGCCTT CAGCTTCCAC AAAGGCTGGA AAAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161094.1
CDS	72..2450
Translation	MGQIFSWIRGTRDQPALQDIAVEQQSQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQSTTIPSTKLGPAKVDPAAGKPSGSAGAQKQTSAQKVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_005161037.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161037.4

ORF Insert Sequence:

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGTCTCA GCAGGTCACA CCCACCACAA CATCTCAGGT CTCCACAGTG 
AAACCTGCAC AATATGAGAA AGGAGCTACA CAATCGTCTA CAGCAGCTGC GACTGTCAAA 
TCCGGCGCTG GAGATCCTGC AACCACCCAG AAAGGATCTT CTCAAGTCAA ACCTCAGAGC 
ACCACCATTC CTTCAACCAA ACTGGGACCT GCTAAGGTGG ATCCGGCTGC TGGGAAGCCC 
TCTGGCTCAG CTGGTGCCCA AAAACAGACA AGTGCTCAAA AGGTGCAGGT TGAAGTGGGT 
CCTTCAGCAG CAAAAGTCAG TGCAGATGCT GACGATCCGT TTGATGCCCT GTCTGGCACA 
TTACCATCAT CACAACCTTT GGCTCCTAAA GTCCACTACA CTGGACCTGA GATCAAAGAG 
CCCAAACTGA AATCAGAGAA AGGTGTTTTG TGTGGTGAGC GAGATGACAC GCTGCCGCCC 
GGATACAGAA AGGAAGACCT GGAAAAGAAG CATGGAGCTG GAGTTCCAGC TGGAGTTCCG 
GAGAAGCCTA AAGAAGTTCC CAAGCCCATA AGCACAGATG ATGCACTGGA ATCTCTCTCC 
TCTACGTTTG TGTCTCCAGC TCAAGCTGCT CCAAAGAAGA CAGACGTGAA AACTGAAACA 
ATAGGAGCAG TGGATGTTAA ATCGGCAGGC ACCACTAACT TTGCTCCCCC TCCACCATCT 
CAGCAGAAAC AACCGGCGTC GTCTCAAGCT GCTGCTGTAA CCAAATCACC TGCTCCACCG 
GCAGACAAGA AAGCTAAAAT GGAGACTCAT GCACAACCAG CCAAAGCAAA GACAGAGTCG 
GACAGCTCCA TATCGCTGGA CGCTCTCGAT GCTTTGAGCG ACACACTGGG GGCTTCTAAA 
CCACCCCAAA AATTACCAGA ACTCAAACCT GGACAGATAG TTGATGAGAA GAAACAGACC 
TCAGAGAAAG GTGTTCGTGT TGGAGAAAGA GACGACACAC TTCCACCACA ATACAGATTC 
AAAGAGGAAG ATCTCAAGAA ATATCCTCCA CCTGCGAAAG AGCCTTCACT GAACACAGAT 
GATGCGATGG ACATACTTTC AGGAGATTTC ACGACCCCTG CATCGGCACC TGTTGTCAAA 
GCTCCTGTTC CTCCTGCACA GGACACAAAG AAGCATGCTG CAGTGCCTGA GAAGACCAAA 
GATATTCCCA AGACTAAAGC TGATGCTCTG TCAAGTGATT TCGTGGCTCC GGCACATTCT 
GCTCCTAAGG TTTCTTCAGC TGCTCCTAAA ACCATCCCTC CAGGCCCACC ACAAACCCCA 
GATGAGGATC CTTTCAGTGC TTTGGGCGAC ACTCTTTCTG CCCCAGAACC ACCAAAAAAA 
CAACCTGACC TGAAACCTAA GGACATCGTA CATGAGAAGG ATGTGACGTC AAAGAAAGGA 
GTTCGTGTTG GAGAAAGAGA CGACACACTC CCACCAGAAT ACAGATTCAA AGAGGAAGAT 
CTCAAGAAAT ATCCTCCTCC TCAGAAAGAT CCTTCCTTAG ATGCTAATGA AGCTCTGGAT 
ATTCTGTCTG GGGATTTTAC AACTCCTTCC GCACCATCGG CTGTTTCCCC TGCCTCTAAA 
TCCTCTGCCA AGTCTTCAGA TTCAGCTTTA GATGCTCTTG CTGGTGATTT CGTCGCTTCT 
TCTTCTGCGT CTTCAGTTCA TTCTGCCGTC TCTGGCCCCC CACGCTCCGA CAGACTGTTG 
TCAGACGGAA CCTCATCAGC TCTAGATGCC CTGTCAGAAA CTCTGGGAGA CATTAAAGCA 
GCCCCTGAAC CCGCCCCTGT CCTACCTAAA ACTGTCGTTA AGGAGAAGAC GGTCATAGAA 
GAGAGAGTGA GTAAACCGGG TGAGAGAGAC GACTCCCTTC CACCAGATCA GCGGTTCACT 
GCGGAGGATC GCAAGGCATT TGAAGAAGCA AAGAAGAAAG ACGCCAAACC AAAACAGCCA 
TCAATGGACG ATTCAGCGGC TCTGGACATG TTGTCTAGTG ATTTTTCAGA TGTACCGACT 
GTAAAGCCCA CTGTATCTGA TGGCAAAAGC TTCACCCCTG AACCAACACC ACCAACCTTT 
AAGGCAACCG GTCCGGTTTT AGACAAACTG GCAGACAACG TGCTTTCCAA CCTGACTGAC 
CCCAAAGCCA AAGACAGCAA AGCAAAGGGA AAGGGGGCGA AACCAAAGTC TAAACCAAAG 
AAACAGGCAG TGGAAGACTC TTCAGCCACA GATCTGCTGT CCAGTAAACC GAGCTCAGAT 
GTGGTGCCTT CAGCTTCCAC AAAGGCTGGA AAAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_005161038.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161095.1 Danio rerio calpastatin (cast), transcript variant X16, mRNA.

Price & Availability↑

CloneID	ODa25615
Clone ID Related Accession (Same CDS sequence)	XM_005161038.3
Accession Version	XM_005161038.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	calpastatin isoform X16
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005161038.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCACA AAAAGAGATG TCATGGTCGT AAGAAAAACA AAAAGGAGAA TGGAGACACT 
TCATTTCAAA AACAAGAATA CGCTCCACAG ACATCAAGGT TCATGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGAA AGGAGCTACA 
CAATCGTCTA CAGCAGCTGC GACTGTCAAA TCCGGCGCTG GAGATCCTGC AACCACCCAG 
AAAGGATCTT CTCAAGTCAA ACCTCAGGTG CAGGTTGAAG TGGGTCCTTC AGCAGCAAAA 
GTCAGTGCAG ATGCTGACGA TCCGTTTGAT GCCCTGTCTG GCACATTACC ATCATCACAA 
CCTTTGGCTC CTAAAGTCCA CTACACTGGA CCTGAGATCA AAGAGCCCAA ACTGAAATCA 
GAGAAAGGTG TTTTGTGTGG TGAGCGAGAT GACACGCTGC CGCCCGGATA CAGAAAGGAA 
GACCTGGAAA AGAAGCATGG AGCTGGAGTT CCAGCTGGAG TTCCGGAGAA GCCTAAAGAA 
GTTCCCAAGC CCATAAGCAC AGATGATGCA CTGGAATCTC TCTCCTCTAC GTTTGTGTCT 
CCAGCTCAAG CTGCTCCAAA GAAGACAGAC GTGAAAACTG AAACAATAGG AGCAGTGGAT 
GTTAAATCGG CAGGCACCAC TAACTTTGCT CCCCCTCCAC CATCTCAGCA GAAACAACCG 
GCGTCGTCTC AAGCTGCTGC TGTAACCAAA TCACCTGCTC CACCGGCAGA CAAGAAAGCT 
AAAATGGAGA CTCATGCACA ACCAGCCAAA GCAAAGACAG AGTCGGACAG CTCCATATCG 
CTGGACGCTC TCGATGCTTT GAGCGACACA CTGGGGGCTT CTAAACCACC CCAAAAATTA 
CCAGAACTCA AACCTGGACA GATAGTTGAT GAGAAGAAAC AGACCTCAGA GAAAGGTGTT 
CGTGTTGGAG AAAGAGACGA CACACTTCCA CCACAATACA GATTCAAAGA GGAAGATCTC 
AAGAAATATC CTCCACCTGC GAAAGAGCCT TCACTGAACA CAGATGATGC GATGGACATA 
CTTTCAGGAG ATTTCACGAC CCCTGCATCG GCACCTGTTG TCAAAGCTCC TGTTCCTCCT 
GCACAGGACA CAAAGAAGCA TGCTGCAGTG CCTGAGAAGA CCAAAGATAT TCCCAAGACT 
AAAGCTGATG CTCTGTCAAG TGATTTCGTG GCTCCGGCAC ATTCTGCTCC TAAGGTTTCT 
TCAGCTGCTC CTAAAACCAT CCCTCCAGGC CCACCACAAA CCCCAGATGA GGATCCTTTC 
AGTGCTTTGG GCGACACTCT TTCTGCCCCA GAACCACCAA AAAAACAACC TGACCTGAAA 
CCTAAGGACA TCGTACATGA GAAGGATGTG ACGTCAAAGA AAGGAGTTCG TGTTGGAGAA 
AGAGACGACA CACTCCCACC AGAATACAGA TTCAAAGAGG AAGATCTCAA GAAATATCCT 
CCTCCTCAGA AAGATCCTTC CTTAGATGCT AATGAAGCTC TGGATATTCT GTCTGGGGAT 
TTTACAACTC CTTCCGCACC ATCGGCTGTT TCCCCTGCCT CTAAATCCTC TGCCAAGTCT 
TCAGATTCAG CTTTAGATGC TCTTGCTGGT GATTTCGTCG CTTCTTCTTC TGCGTCTTCA 
GTTCATTCTG CCGTCTCTGG CCCCCCACGC TCCGACAGAC TGTTGTCAGA CGGAACCTCA 
TCAGCTCTAG ATGCCCTGTC AGAAACTCTG GGAGACATTA AAGCAGCCCC TGAACCCGCC 
CCTGTCCTAC CTAAAACTGT CGTTAAGGAG AAGACGGTCA TAGAAGAGAG AGTGAGTAAA 
CCGGGTGAGA GAGACGACTC CCTTCCACCA GATCAGCGGT TCACTGCGGA GGATCGCAAG 
GCATTTGAAG AAGCAAAGAA GAAAGACGCC AAACCAAAAC AGCCATCAAT GGACGATTCA 
GCGGCTCTGG ACATGTTGTC TAGTGATTTT TCAGATGTAC CGACTGTAAA GCCCACTGTA 
TCTGATGGCA AAAGCTTCAC CCCTGAACCA ACACCACCAA CCTTTAAGGC AACCGGTCCG 
GTTTTAGACA AACTGGCAGA CAACGTGCTT TCCAACCTGA CTGACCCCAA AGCCAAAGAC 
AGCAAAGCAA AGGGAAAGGG GGCGAAACCA AAGTCTAAAC CAAAGAAACA GGCAGTGGAA 
GACTCTTCAG CCACAGATCT GCTGTCCAGT AAACCGAGCT CAGATGTGGT GCCTTCAGCT 
TCCACAAAGG CTGGAAAAAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161095.1
CDS	39..2342
Translation	MPHKKRCHGRKKNKKENGDTSFQKQEYAPQTSRFMSQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_005161038.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161038.3

ORF Insert Sequence:

ATGCCGCACA AAAAGAGATG TCATGGTCGT AAGAAAAACA AAAAGGAGAA TGGAGACACT 
TCATTTCAAA AACAAGAATA CGCTCCACAG ACATCAAGGT TCATGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGAA AGGAGCTACA 
CAATCGTCTA CAGCAGCTGC GACTGTCAAA TCCGGCGCTG GAGATCCTGC AACCACCCAG 
AAAGGATCTT CTCAAGTCAA ACCTCAGGTG CAGGTTGAAG TGGGTCCTTC AGCAGCAAAA 
GTCAGTGCAG ATGCTGACGA TCCGTTTGAT GCCCTGTCTG GCACATTACC ATCATCACAA 
CCTTTGGCTC CTAAAGTCCA CTACACTGGA CCTGAGATCA AAGAGCCCAA ACTGAAATCA 
GAGAAAGGTG TTTTGTGTGG TGAGCGAGAT GACACGCTGC CGCCCGGATA CAGAAAGGAA 
GACCTGGAAA AGAAGCATGG AGCTGGAGTT CCAGCTGGAG TTCCGGAGAA GCCTAAAGAA 
GTTCCCAAGC CCATAAGCAC AGATGATGCA CTGGAATCTC TCTCCTCTAC GTTTGTGTCT 
CCAGCTCAAG CTGCTCCAAA GAAGACAGAC GTGAAAACTG AAACAATAGG AGCAGTGGAT 
GTTAAATCGG CAGGCACCAC TAACTTTGCT CCCCCTCCAC CATCTCAGCA GAAACAACCG 
GCGTCGTCTC AAGCTGCTGC TGTAACCAAA TCACCTGCTC CACCGGCAGA CAAGAAAGCT 
AAAATGGAGA CTCATGCACA ACCAGCCAAA GCAAAGACAG AGTCGGACAG CTCCATATCG 
CTGGACGCTC TCGATGCTTT GAGCGACACA CTGGGGGCTT CTAAACCACC CCAAAAATTA 
CCAGAACTCA AACCTGGACA GATAGTTGAT GAGAAGAAAC AGACCTCAGA GAAAGGTGTT 
CGTGTTGGAG AAAGAGACGA CACACTTCCA CCACAATACA GATTCAAAGA GGAAGATCTC 
AAGAAATATC CTCCACCTGC GAAAGAGCCT TCACTGAACA CAGATGATGC GATGGACATA 
CTTTCAGGAG ATTTCACGAC CCCTGCATCG GCACCTGTTG TCAAAGCTCC TGTTCCTCCT 
GCACAGGACA CAAAGAAGCA TGCTGCAGTG CCTGAGAAGA CCAAAGATAT TCCCAAGACT 
AAAGCTGATG CTCTGTCAAG TGATTTCGTG GCTCCGGCAC ATTCTGCTCC TAAGGTTTCT 
TCAGCTGCTC CTAAAACCAT CCCTCCAGGC CCACCACAAA CCCCAGATGA GGATCCTTTC 
AGTGCTTTGG GCGACACTCT TTCTGCCCCA GAACCACCAA AAAAACAACC TGACCTGAAA 
CCTAAGGACA TCGTACATGA GAAGGATGTG ACGTCAAAGA AAGGAGTTCG TGTTGGAGAA 
AGAGACGACA CACTCCCACC AGAATACAGA TTCAAAGAGG AAGATCTCAA GAAATATCCT 
CCTCCTCAGA AAGATCCTTC CTTAGATGCT AATGAAGCTC TGGATATTCT GTCTGGGGAT 
TTTACAACTC CTTCCGCACC ATCGGCTGTT TCCCCTGCCT CTAAATCCTC TGCCAAGTCT 
TCAGATTCAG CTTTAGATGC TCTTGCTGGT GATTTCGTCG CTTCTTCTTC TGCGTCTTCA 
GTTCATTCTG CCGTCTCTGG CCCCCCACGC TCCGACAGAC TGTTGTCAGA CGGAACCTCA 
TCAGCTCTAG ATGCCCTGTC AGAAACTCTG GGAGACATTA AAGCAGCCCC TGAACCCGCC 
CCTGTCCTAC CTAAAACTGT CGTTAAGGAG AAGACGGTCA TAGAAGAGAG AGTGAGTAAA 
CCGGGTGAGA GAGACGACTC CCTTCCACCA GATCAGCGGT TCACTGCGGA GGATCGCAAG 
GCATTTGAAG AAGCAAAGAA GAAAGACGCC AAACCAAAAC AGCCATCAAT GGACGATTCA 
GCGGCTCTGG ACATGTTGTC TAGTGATTTT TCAGATGTAC CGACTGTAAA GCCCACTGTA 
TCTGATGGCA AAAGCTTCAC CCCTGAACCA ACACCACCAA CCTTTAAGGC AACCGGTCCG 
GTTTTAGACA AACTGGCAGA CAACGTGCTT TCCAACCTGA CTGACCCCAA AGCCAAAGAC 
AGCAAAGCAA AGGGAAAGGG GGCGAAACCA AAGTCTAAAC CAAAGAAACA GGCAGTGGAA 
GACTCTTCAG CCACAGATCT GCTGTCCAGT AAACCGAGCT CAGATGTGGT GCCTTCAGCT 
TCCACAAAGG CTGGAAAAAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_005161040.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161097.1 Danio rerio calpastatin (cast), transcript variant X19, mRNA.

Price & Availability↑

CloneID	ODa25617
Clone ID Related Accession (Same CDS sequence)	XM_005161040.4 , XM_005161040.3
Accession Version	XM_005161040.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calpastatin isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005161040.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGTCTCA GCAGGTCACA CCCACCACAA CATCTCAGGT CTCCACAGTG 
AAACCTGCAC AATATGAGAA AGGAGCTACA CAATCGTCTA CAGCAGCTGC GACTGTCAAA 
TCCGGCGCTG GAGATCCTGC AACCACCCAG AAAGGATCTT CTCAAGTCAA ACCTCAGGTG 
CAGGTTGAAG TGGGTCCTTC AGCAGCAAAA GTCAGTGCAG ATGCTGACGA TCCGTTTGAT 
GCCCTGTCTG GCACATTACC ATCATCACAA CCTTTGGCTC CTAAAGTCCA CTACACTGGA 
CCTGAGATCA AAGAGCCCAA ACTGAAATCA GAGAAAGGTG TTTTGTGTGG TGAGCGAGAT 
GACACGCTGC CGCCCGGATA CAGAAAGGAA GACCTGGAAA AGAAGCATGG AGCTGGAGTT 
CCAGCTGGAG TTCCGGAGAA GCCTAAAGAA GTTCCCAAGC CCATAAGCAC AGATGATGCA 
CTGGAATCTC TCTCCTCTAC GTTTGTGTCT CCAGCTCAAG CTGCTCCAAA GAAGACAGAC 
GTGAAAACTG AAACAATAGG AGCAGTGGAT GTTAAATCGG CAGGCACCAC TAACTTTGCT 
CCCCCTCCAC CATCTCAGCA GAAACAACCG GCGTCGTCTC AAGCTGCTGC TGTAACCAAA 
TCACCTGCTC CACCGGCAGA CAAGAAAGCT AAAATGGAGA CTCATGCACA ACCAGCCAAA 
GCAAAGACAG AGTCGGACAG CTCCATATCG CTGGACGCTC TCGATGCTTT GAGCGACACA 
CTGGGGGCTT CTAAACCACC CCAAAAATTA CCAGAACTCA AACCTGGACA GATAGTTGAT 
GAGAAGAAAC AGACCTCAGA GAAAGGTGTT CGTGTTGGAG AAAGAGACGA CACACTTCCA 
CCACAATACA GATTCAAAGA GGAAGATCTC AAGAAATATC CTCCACCTGC GAAAGAGCCT 
TCACTGAACA CAGATGATGC GATGGACATA CTTTCAGGAG ATTTCACGAC CCCTGCATCG 
GCACCTGTTG TCAAAGCTCC TGTTCCTCCT GCACAGGACA CAAAGAAGCA TGCTGCAGTG 
CCTGAGAAGA CCAAAGATAT TCCCAAGACT AAAGCTGATG CTCTGTCAAG TGATTTCGTG 
GCTCCGGCAC ATTCTGCTCC TAAGGTTTCT TCAGCTGCTC CTAAAACCAT CCCTCCAGGC 
CCACCACAAA CCCCAGATGA GGATCCTTTC AGTGCTTTGG GCGACACTCT TTCTGCCCCA 
GAACCACCAA AAAAACAACC TGACCTGAAA CCTAAGGACA TCGTACATGA GAAGGATGTG 
ACGTCAAAGA AAGGAGTTCG TGTTGGAGAA AGAGACGACA CACTCCCACC AGAATACAGA 
TTCAAAGAGG AAGATCTCAA GAAATATCCT CCTCCTCAGA AAGATCCTTC CTTAGATGCT 
AATGAAGCTC TGGATATTCT GTCTGGGGAT TTTACAACTC CTTCCGCACC ATCGGCTGTT 
TCCCCTGCCT CTAAATCCTC TGCCAAGTCT TCAGATTCAG CTTTAGATGC TCTTGCTGGT 
GATTTCGTCG CTTCTTCTTC TGCGTCTTCA GTTCATTCTG CCGTCTCTGG CCCCCCACGC 
TCCGACAGAC TGTTGTCAGA CGGAACCTCA TCAGCTCTAG ATGCCCTGTC AGAAACTCTG 
GGAGACATTA AAGCAGCCCC TGAACCCGCC CCTGTCCTAC CTAAAACTGT CGTTAAGGAG 
AAGACGGTCA TAGAAGAGAG AGTGAGTAAA CCGGGTGAGA GAGACGACTC CCTTCCACCA 
GATCAGCGGT TCACTGCGGA GGATCGCAAG GCATTTGAAG AAGCAAAGAA GAAAGACGCC 
AAACCAAAAC AGCCATCAAT GGACGATTCA GCGGCTCTGG ACATGTTGTC TAGTGATTTT 
TCAGATGTAC CGACTGTAAA GCCCACTGTA TCTGATGGCA AAAGCTTCAC CCCTGAACCA 
ACACCACCAA CCTTTAAGGC AACCGGTCCG GTTTTAGACA AACTGGCAGA CAACGTGCTT 
TCCAACCTGA CTGACCCCAA AGCCAAAGAC AGCAAAGCAA AGGGAAAGGG GGCGAAACCA 
AAGTCTAAAC CAAAGAAACA GGCAGTGGAA GACTCTTCAG CCACAGATCT GCTGTCCAGT 
AAACCGAGCT CAGATGTGGT GCCTTCAGCT TCCACAAAGG CTGGAAAAAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161097.1
CDS	72..2345
Translation	MGQIFSWIRGTRDQPALQDIAVEQQSQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_005161040.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161040.4

ORF Insert Sequence:

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGTCTCA GCAGGTCACA CCCACCACAA CATCTCAGGT CTCCACAGTG 
AAACCTGCAC AATATGAGAA AGGAGCTACA CAATCGTCTA CAGCAGCTGC GACTGTCAAA 
TCCGGCGCTG GAGATCCTGC AACCACCCAG AAAGGATCTT CTCAAGTCAA ACCTCAGGTG 
CAGGTTGAAG TGGGTCCTTC AGCAGCAAAA GTCAGTGCAG ATGCTGACGA TCCGTTTGAT 
GCCCTGTCTG GCACATTACC ATCATCACAA CCTTTGGCTC CTAAAGTCCA CTACACTGGA 
CCTGAGATCA AAGAGCCCAA ACTGAAATCA GAGAAAGGTG TTTTGTGTGG TGAGCGAGAT 
GACACGCTGC CGCCCGGATA CAGAAAGGAA GACCTGGAAA AGAAGCATGG AGCTGGAGTT 
CCAGCTGGAG TTCCGGAGAA GCCTAAAGAA GTTCCCAAGC CCATAAGCAC AGATGATGCA 
CTGGAATCTC TCTCCTCTAC GTTTGTGTCT CCAGCTCAAG CTGCTCCAAA GAAGACAGAC 
GTGAAAACTG AAACAATAGG AGCAGTGGAT GTTAAATCGG CAGGCACCAC TAACTTTGCT 
CCCCCTCCAC CATCTCAGCA GAAACAACCG GCGTCGTCTC AAGCTGCTGC TGTAACCAAA 
TCACCTGCTC CACCGGCAGA CAAGAAAGCT AAAATGGAGA CTCATGCACA ACCAGCCAAA 
GCAAAGACAG AGTCGGACAG CTCCATATCG CTGGACGCTC TCGATGCTTT GAGCGACACA 
CTGGGGGCTT CTAAACCACC CCAAAAATTA CCAGAACTCA AACCTGGACA GATAGTTGAT 
GAGAAGAAAC AGACCTCAGA GAAAGGTGTT CGTGTTGGAG AAAGAGACGA CACACTTCCA 
CCACAATACA GATTCAAAGA GGAAGATCTC AAGAAATATC CTCCACCTGC GAAAGAGCCT 
TCACTGAACA CAGATGATGC GATGGACATA CTTTCAGGAG ATTTCACGAC CCCTGCATCG 
GCACCTGTTG TCAAAGCTCC TGTTCCTCCT GCACAGGACA CAAAGAAGCA TGCTGCAGTG 
CCTGAGAAGA CCAAAGATAT TCCCAAGACT AAAGCTGATG CTCTGTCAAG TGATTTCGTG 
GCTCCGGCAC ATTCTGCTCC TAAGGTTTCT TCAGCTGCTC CTAAAACCAT CCCTCCAGGC 
CCACCACAAA CCCCAGATGA GGATCCTTTC AGTGCTTTGG GCGACACTCT TTCTGCCCCA 
GAACCACCAA AAAAACAACC TGACCTGAAA CCTAAGGACA TCGTACATGA GAAGGATGTG 
ACGTCAAAGA AAGGAGTTCG TGTTGGAGAA AGAGACGACA CACTCCCACC AGAATACAGA 
TTCAAAGAGG AAGATCTCAA GAAATATCCT CCTCCTCAGA AAGATCCTTC CTTAGATGCT 
AATGAAGCTC TGGATATTCT GTCTGGGGAT TTTACAACTC CTTCCGCACC ATCGGCTGTT 
TCCCCTGCCT CTAAATCCTC TGCCAAGTCT TCAGATTCAG CTTTAGATGC TCTTGCTGGT 
GATTTCGTCG CTTCTTCTTC TGCGTCTTCA GTTCATTCTG CCGTCTCTGG CCCCCCACGC 
TCCGACAGAC TGTTGTCAGA CGGAACCTCA TCAGCTCTAG ATGCCCTGTC AGAAACTCTG 
GGAGACATTA AAGCAGCCCC TGAACCCGCC CCTGTCCTAC CTAAAACTGT CGTTAAGGAG 
AAGACGGTCA TAGAAGAGAG AGTGAGTAAA CCGGGTGAGA GAGACGACTC CCTTCCACCA 
GATCAGCGGT TCACTGCGGA GGATCGCAAG GCATTTGAAG AAGCAAAGAA GAAAGACGCC 
AAACCAAAAC AGCCATCAAT GGACGATTCA GCGGCTCTGG ACATGTTGTC TAGTGATTTT 
TCAGATGTAC CGACTGTAAA GCCCACTGTA TCTGATGGCA AAAGCTTCAC CCCTGAACCA 
ACACCACCAA CCTTTAAGGC AACCGGTCCG GTTTTAGACA AACTGGCAGA CAACGTGCTT 
TCCAACCTGA CTGACCCCAA AGCCAAAGAC AGCAAAGCAA AGGGAAAGGG GGCGAAACCA 
AAGTCTAAAC CAAAGAAACA GGCAGTGGAA GACTCTTCAG CCACAGATCT GCTGTCCAGT 
AAACCGAGCT CAGATGTGGT GCCTTCAGCT TCCACAAAGG CTGGAAAAAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_005161040.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161097.1 Danio rerio calpastatin (cast), transcript variant X18, mRNA.

Price & Availability↑

CloneID	ODa25617
Clone ID Related Accession (Same CDS sequence)	XM_005161040.4 , XM_005161040.3
Accession Version	XM_005161040.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	calpastatin isoform X18
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005161040.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGTCTCA GCAGGTCACA CCCACCACAA CATCTCAGGT CTCCACAGTG 
AAACCTGCAC AATATGAGAA AGGAGCTACA CAATCGTCTA CAGCAGCTGC GACTGTCAAA 
TCCGGCGCTG GAGATCCTGC AACCACCCAG AAAGGATCTT CTCAAGTCAA ACCTCAGGTG 
CAGGTTGAAG TGGGTCCTTC AGCAGCAAAA GTCAGTGCAG ATGCTGACGA TCCGTTTGAT 
GCCCTGTCTG GCACATTACC ATCATCACAA CCTTTGGCTC CTAAAGTCCA CTACACTGGA 
CCTGAGATCA AAGAGCCCAA ACTGAAATCA GAGAAAGGTG TTTTGTGTGG TGAGCGAGAT 
GACACGCTGC CGCCCGGATA CAGAAAGGAA GACCTGGAAA AGAAGCATGG AGCTGGAGTT 
CCAGCTGGAG TTCCGGAGAA GCCTAAAGAA GTTCCCAAGC CCATAAGCAC AGATGATGCA 
CTGGAATCTC TCTCCTCTAC GTTTGTGTCT CCAGCTCAAG CTGCTCCAAA GAAGACAGAC 
GTGAAAACTG AAACAATAGG AGCAGTGGAT GTTAAATCGG CAGGCACCAC TAACTTTGCT 
CCCCCTCCAC CATCTCAGCA GAAACAACCG GCGTCGTCTC AAGCTGCTGC TGTAACCAAA 
TCACCTGCTC CACCGGCAGA CAAGAAAGCT AAAATGGAGA CTCATGCACA ACCAGCCAAA 
GCAAAGACAG AGTCGGACAG CTCCATATCG CTGGACGCTC TCGATGCTTT GAGCGACACA 
CTGGGGGCTT CTAAACCACC CCAAAAATTA CCAGAACTCA AACCTGGACA GATAGTTGAT 
GAGAAGAAAC AGACCTCAGA GAAAGGTGTT CGTGTTGGAG AAAGAGACGA CACACTTCCA 
CCACAATACA GATTCAAAGA GGAAGATCTC AAGAAATATC CTCCACCTGC GAAAGAGCCT 
TCACTGAACA CAGATGATGC GATGGACATA CTTTCAGGAG ATTTCACGAC CCCTGCATCG 
GCACCTGTTG TCAAAGCTCC TGTTCCTCCT GCACAGGACA CAAAGAAGCA TGCTGCAGTG 
CCTGAGAAGA CCAAAGATAT TCCCAAGACT AAAGCTGATG CTCTGTCAAG TGATTTCGTG 
GCTCCGGCAC ATTCTGCTCC TAAGGTTTCT TCAGCTGCTC CTAAAACCAT CCCTCCAGGC 
CCACCACAAA CCCCAGATGA GGATCCTTTC AGTGCTTTGG GCGACACTCT TTCTGCCCCA 
GAACCACCAA AAAAACAACC TGACCTGAAA CCTAAGGACA TCGTACATGA GAAGGATGTG 
ACGTCAAAGA AAGGAGTTCG TGTTGGAGAA AGAGACGACA CACTCCCACC AGAATACAGA 
TTCAAAGAGG AAGATCTCAA GAAATATCCT CCTCCTCAGA AAGATCCTTC CTTAGATGCT 
AATGAAGCTC TGGATATTCT GTCTGGGGAT TTTACAACTC CTTCCGCACC ATCGGCTGTT 
TCCCCTGCCT CTAAATCCTC TGCCAAGTCT TCAGATTCAG CTTTAGATGC TCTTGCTGGT 
GATTTCGTCG CTTCTTCTTC TGCGTCTTCA GTTCATTCTG CCGTCTCTGG CCCCCCACGC 
TCCGACAGAC TGTTGTCAGA CGGAACCTCA TCAGCTCTAG ATGCCCTGTC AGAAACTCTG 
GGAGACATTA AAGCAGCCCC TGAACCCGCC CCTGTCCTAC CTAAAACTGT CGTTAAGGAG 
AAGACGGTCA TAGAAGAGAG AGTGAGTAAA CCGGGTGAGA GAGACGACTC CCTTCCACCA 
GATCAGCGGT TCACTGCGGA GGATCGCAAG GCATTTGAAG AAGCAAAGAA GAAAGACGCC 
AAACCAAAAC AGCCATCAAT GGACGATTCA GCGGCTCTGG ACATGTTGTC TAGTGATTTT 
TCAGATGTAC CGACTGTAAA GCCCACTGTA TCTGATGGCA AAAGCTTCAC CCCTGAACCA 
ACACCACCAA CCTTTAAGGC AACCGGTCCG GTTTTAGACA AACTGGCAGA CAACGTGCTT 
TCCAACCTGA CTGACCCCAA AGCCAAAGAC AGCAAAGCAA AGGGAAAGGG GGCGAAACCA 
AAGTCTAAAC CAAAGAAACA GGCAGTGGAA GACTCTTCAG CCACAGATCT GCTGTCCAGT 
AAACCGAGCT CAGATGTGGT GCCTTCAGCT TCCACAAAGG CTGGAAAAAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161097.1
CDS	67..2340
Translation	MGQIFSWIRGTRDQPALQDIAVEQQSQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_005161040.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161040.3

ORF Insert Sequence:

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGTCTCA GCAGGTCACA CCCACCACAA CATCTCAGGT CTCCACAGTG 
AAACCTGCAC AATATGAGAA AGGAGCTACA CAATCGTCTA CAGCAGCTGC GACTGTCAAA 
TCCGGCGCTG GAGATCCTGC AACCACCCAG AAAGGATCTT CTCAAGTCAA ACCTCAGGTG 
CAGGTTGAAG TGGGTCCTTC AGCAGCAAAA GTCAGTGCAG ATGCTGACGA TCCGTTTGAT 
GCCCTGTCTG GCACATTACC ATCATCACAA CCTTTGGCTC CTAAAGTCCA CTACACTGGA 
CCTGAGATCA AAGAGCCCAA ACTGAAATCA GAGAAAGGTG TTTTGTGTGG TGAGCGAGAT 
GACACGCTGC CGCCCGGATA CAGAAAGGAA GACCTGGAAA AGAAGCATGG AGCTGGAGTT 
CCAGCTGGAG TTCCGGAGAA GCCTAAAGAA GTTCCCAAGC CCATAAGCAC AGATGATGCA 
CTGGAATCTC TCTCCTCTAC GTTTGTGTCT CCAGCTCAAG CTGCTCCAAA GAAGACAGAC 
GTGAAAACTG AAACAATAGG AGCAGTGGAT GTTAAATCGG CAGGCACCAC TAACTTTGCT 
CCCCCTCCAC CATCTCAGCA GAAACAACCG GCGTCGTCTC AAGCTGCTGC TGTAACCAAA 
TCACCTGCTC CACCGGCAGA CAAGAAAGCT AAAATGGAGA CTCATGCACA ACCAGCCAAA 
GCAAAGACAG AGTCGGACAG CTCCATATCG CTGGACGCTC TCGATGCTTT GAGCGACACA 
CTGGGGGCTT CTAAACCACC CCAAAAATTA CCAGAACTCA AACCTGGACA GATAGTTGAT 
GAGAAGAAAC AGACCTCAGA GAAAGGTGTT CGTGTTGGAG AAAGAGACGA CACACTTCCA 
CCACAATACA GATTCAAAGA GGAAGATCTC AAGAAATATC CTCCACCTGC GAAAGAGCCT 
TCACTGAACA CAGATGATGC GATGGACATA CTTTCAGGAG ATTTCACGAC CCCTGCATCG 
GCACCTGTTG TCAAAGCTCC TGTTCCTCCT GCACAGGACA CAAAGAAGCA TGCTGCAGTG 
CCTGAGAAGA CCAAAGATAT TCCCAAGACT AAAGCTGATG CTCTGTCAAG TGATTTCGTG 
GCTCCGGCAC ATTCTGCTCC TAAGGTTTCT TCAGCTGCTC CTAAAACCAT CCCTCCAGGC 
CCACCACAAA CCCCAGATGA GGATCCTTTC AGTGCTTTGG GCGACACTCT TTCTGCCCCA 
GAACCACCAA AAAAACAACC TGACCTGAAA CCTAAGGACA TCGTACATGA GAAGGATGTG 
ACGTCAAAGA AAGGAGTTCG TGTTGGAGAA AGAGACGACA CACTCCCACC AGAATACAGA 
TTCAAAGAGG AAGATCTCAA GAAATATCCT CCTCCTCAGA AAGATCCTTC CTTAGATGCT 
AATGAAGCTC TGGATATTCT GTCTGGGGAT TTTACAACTC CTTCCGCACC ATCGGCTGTT 
TCCCCTGCCT CTAAATCCTC TGCCAAGTCT TCAGATTCAG CTTTAGATGC TCTTGCTGGT 
GATTTCGTCG CTTCTTCTTC TGCGTCTTCA GTTCATTCTG CCGTCTCTGG CCCCCCACGC 
TCCGACAGAC TGTTGTCAGA CGGAACCTCA TCAGCTCTAG ATGCCCTGTC AGAAACTCTG 
GGAGACATTA AAGCAGCCCC TGAACCCGCC CCTGTCCTAC CTAAAACTGT CGTTAAGGAG 
AAGACGGTCA TAGAAGAGAG AGTGAGTAAA CCGGGTGAGA GAGACGACTC CCTTCCACCA 
GATCAGCGGT TCACTGCGGA GGATCGCAAG GCATTTGAAG AAGCAAAGAA GAAAGACGCC 
AAACCAAAAC AGCCATCAAT GGACGATTCA GCGGCTCTGG ACATGTTGTC TAGTGATTTT 
TCAGATGTAC CGACTGTAAA GCCCACTGTA TCTGATGGCA AAAGCTTCAC CCCTGAACCA 
ACACCACCAA CCTTTAAGGC AACCGGTCCG GTTTTAGACA AACTGGCAGA CAACGTGCTT 
TCCAACCTGA CTGACCCCAA AGCCAAAGAC AGCAAAGCAA AGGGAAAGGG GGCGAAACCA 
AAGTCTAAAC CAAAGAAACA GGCAGTGGAA GACTCTTCAG CCACAGATCT GCTGTCCAGT 
AAACCGAGCT CAGATGTGGT GCCTTCAGCT TCCACAAAGG CTGGAAAAAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_005161041.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161098.1 Danio rerio calpastatin (cast), transcript variant X21, mRNA.

Price & Availability↑

CloneID	ODa25618
Clone ID Related Accession (Same CDS sequence)	XM_005161041.4 , XM_005161041.3
Accession Version	XM_005161041.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calpastatin isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005161041.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCACA AAAAGAGATG TCATGGTCGT AAGAAAAACA AAAAGGAGAA TGGAGACACT 
TCATTTCAAA AACAAGAATA CGCTCCACAG ACATCAAGGT TCATGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGGT GCAGGTTGAA 
GTGGGTCCTT CAGCAGCAAA AGTCAGTGCA GATGCTGACG ATCCGTTTGA TGCCCTGTCT 
GGCACATTAC CATCATCACA ACCTTTGGCT CCTAAAGTCC ACTACACTGG ACCTGAGATC 
AAAGAGCCCA AACTGAAATC AGAGAAAGGT GTTTTGTGTG GTGAGCGAGA TGACACGCTG 
CCGCCCGGAT ACAGAAAGGA AGACCTGGAA AAGAAGCATG GAGCTGGAGT TCCAGCTGGA 
GTTCCGGAGA AGCCTAAAGA AGTTCCCAAG CCCATAAGCA CAGATGATGC ACTGGAATCT 
CTCTCCTCTA CGTTTGTGTC TCCAGCTCAA GCTGCTCCAA AGAAGACAGA CGTGAAAACT 
GAAACAATAG GAGCAGTGGA TGTTAAATCG GCAGGCACCA CTAACTTTGC TCCCCCTCCA 
CCATCTCAGC AGAAACAACC GGCGTCGTCT CAAGCTGCTG CTGTAACCAA ATCACCTGCT 
CCACCGGCAG ACAAGAAAGC TAAAATGGAG ACTCATGCAC AACCAGCCAA AGCAAAGACA 
GAGTCGGACA GCTCCATATC GCTGGACGCT CTCGATGCTT TGAGCGACAC ACTGGGGGCT 
TCTAAACCAC CCCAAAAATT ACCAGAACTC AAACCTGGAC AGATAGTTGA TGAGAAGAAA 
CAGACCTCAG AGAAAGGTGT TCGTGTTGGA GAAAGAGACG ACACACTTCC ACCACAATAC 
AGATTCAAAG AGGAAGATCT CAAGAAATAT CCTCCACCTG CGAAAGAGCC TTCACTGAAC 
ACAGATGATG CGATGGACAT ACTTTCAGGA GATTTCACGA CCCCTGCATC GGCACCTGTT 
GTCAAAGCTC CTGTTCCTCC TGCACAGGAC ACAAAGAAGC ATGCTGCAGT GCCTGAGAAG 
ACCAAAGATA TTCCCAAGAC TAAAGCTGAT GCTCTGTCAA GTGATTTCGT GGCTCCGGCA 
CATTCTGCTC CTAAGGTTTC TTCAGCTGCT CCTAAAACCA TCCCTCCAGG CCCACCACAA 
ACCCCAGATG AGGATCCTTT CAGTGCTTTG GGCGACACTC TTTCTGCCCC AGAACCACCA 
AAAAAACAAC CTGACCTGAA ACCTAAGGAC ATCGTACATG AGAAGGATGT GACGTCAAAG 
AAAGGAGTTC GTGTTGGAGA AAGAGACGAC ACACTCCCAC CAGAATACAG ATTCAAAGAG 
GAAGATCTCA AGAAATATCC TCCTCCTCAG AAAGATCCTT CCTTAGATGC TAATGAAGCT 
CTGGATATTC TGTCTGGGGA TTTTACAACT CCTTCCGCAC CATCGGCTGT TTCCCCTGCC 
TCTAAATCCT CTGCCAAGTC TTCAGATTCA GCTTTAGATG CTCTTGCTGG TGATTTCGTC 
GCTTCTTCTT CTGCGTCTTC AGTTCATTCT GCCGTCTCTG GCCCCCCACG CTCCGACAGA 
CTGTTGTCAG ACGGAACCTC ATCAGCTCTA GATGCCCTGT CAGAAACTCT GGGAGACATT 
AAAGCAGCCC CTGAACCCGC CCCTGTCCTA CCTAAAACTG TCGTTAAGGA GAAGACGGTC 
ATAGAAGAGA GAGTGAGTAA ACCGGGTGAG AGAGACGACT CCCTTCCACC AGATCAGCGG 
TTCACTGCGG AGGATCGCAA GGCATTTGAA GAAGCAAAGA AGAAAGACGC CAAACCAAAA 
CAGCCATCAA TGGACGATTC AGCGGCTCTG GACATGTTGT CTAGTGATTT TTCAGATGTA 
CCGACTGTAA AGCCCACTGT ATCTGATGGC AAAAGCTTCA CCCCTGAACC AACACCACCA 
ACCTTTAAGG CAACCGGTCC GGTTTTAGAC AAACTGGCAG ACAACGTGCT TTCCAACCTG 
ACTGACCCCA AAGCCAAAGA CAGCAAAGCA AAGGGAAAGG GGGCGAAACC AAAGTCTAAA 
CCAAAGAAAC AGGCAGTGGA AGACTCTTCA GCCACAGATC TGCTGTCCAG TAAACCGAGC 
TCAGATGTGG TGCCTTCAGC TTCCACAAAG GCTGGAAAAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161098.1
CDS	24..2228
Translation	MPHKKRCHGRKKNKKENGDTSFQKQEYAPQTSRFMSQQVTPTTTSQVSTVKPAQYEVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_005161041.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161041.4

ORF Insert Sequence:

ATGCCGCACA AAAAGAGATG TCATGGTCGT AAGAAAAACA AAAAGGAGAA TGGAGACACT 
TCATTTCAAA AACAAGAATA CGCTCCACAG ACATCAAGGT TCATGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGGT GCAGGTTGAA 
GTGGGTCCTT CAGCAGCAAA AGTCAGTGCA GATGCTGACG ATCCGTTTGA TGCCCTGTCT 
GGCACATTAC CATCATCACA ACCTTTGGCT CCTAAAGTCC ACTACACTGG ACCTGAGATC 
AAAGAGCCCA AACTGAAATC AGAGAAAGGT GTTTTGTGTG GTGAGCGAGA TGACACGCTG 
CCGCCCGGAT ACAGAAAGGA AGACCTGGAA AAGAAGCATG GAGCTGGAGT TCCAGCTGGA 
GTTCCGGAGA AGCCTAAAGA AGTTCCCAAG CCCATAAGCA CAGATGATGC ACTGGAATCT 
CTCTCCTCTA CGTTTGTGTC TCCAGCTCAA GCTGCTCCAA AGAAGACAGA CGTGAAAACT 
GAAACAATAG GAGCAGTGGA TGTTAAATCG GCAGGCACCA CTAACTTTGC TCCCCCTCCA 
CCATCTCAGC AGAAACAACC GGCGTCGTCT CAAGCTGCTG CTGTAACCAA ATCACCTGCT 
CCACCGGCAG ACAAGAAAGC TAAAATGGAG ACTCATGCAC AACCAGCCAA AGCAAAGACA 
GAGTCGGACA GCTCCATATC GCTGGACGCT CTCGATGCTT TGAGCGACAC ACTGGGGGCT 
TCTAAACCAC CCCAAAAATT ACCAGAACTC AAACCTGGAC AGATAGTTGA TGAGAAGAAA 
CAGACCTCAG AGAAAGGTGT TCGTGTTGGA GAAAGAGACG ACACACTTCC ACCACAATAC 
AGATTCAAAG AGGAAGATCT CAAGAAATAT CCTCCACCTG CGAAAGAGCC TTCACTGAAC 
ACAGATGATG CGATGGACAT ACTTTCAGGA GATTTCACGA CCCCTGCATC GGCACCTGTT 
GTCAAAGCTC CTGTTCCTCC TGCACAGGAC ACAAAGAAGC ATGCTGCAGT GCCTGAGAAG 
ACCAAAGATA TTCCCAAGAC TAAAGCTGAT GCTCTGTCAA GTGATTTCGT GGCTCCGGCA 
CATTCTGCTC CTAAGGTTTC TTCAGCTGCT CCTAAAACCA TCCCTCCAGG CCCACCACAA 
ACCCCAGATG AGGATCCTTT CAGTGCTTTG GGCGACACTC TTTCTGCCCC AGAACCACCA 
AAAAAACAAC CTGACCTGAA ACCTAAGGAC ATCGTACATG AGAAGGATGT GACGTCAAAG 
AAAGGAGTTC GTGTTGGAGA AAGAGACGAC ACACTCCCAC CAGAATACAG ATTCAAAGAG 
GAAGATCTCA AGAAATATCC TCCTCCTCAG AAAGATCCTT CCTTAGATGC TAATGAAGCT 
CTGGATATTC TGTCTGGGGA TTTTACAACT CCTTCCGCAC CATCGGCTGT TTCCCCTGCC 
TCTAAATCCT CTGCCAAGTC TTCAGATTCA GCTTTAGATG CTCTTGCTGG TGATTTCGTC 
GCTTCTTCTT CTGCGTCTTC AGTTCATTCT GCCGTCTCTG GCCCCCCACG CTCCGACAGA 
CTGTTGTCAG ACGGAACCTC ATCAGCTCTA GATGCCCTGT CAGAAACTCT GGGAGACATT 
AAAGCAGCCC CTGAACCCGC CCCTGTCCTA CCTAAAACTG TCGTTAAGGA GAAGACGGTC 
ATAGAAGAGA GAGTGAGTAA ACCGGGTGAG AGAGACGACT CCCTTCCACC AGATCAGCGG 
TTCACTGCGG AGGATCGCAA GGCATTTGAA GAAGCAAAGA AGAAAGACGC CAAACCAAAA 
CAGCCATCAA TGGACGATTC AGCGGCTCTG GACATGTTGT CTAGTGATTT TTCAGATGTA 
CCGACTGTAA AGCCCACTGT ATCTGATGGC AAAAGCTTCA CCCCTGAACC AACACCACCA 
ACCTTTAAGG CAACCGGTCC GGTTTTAGAC AAACTGGCAG ACAACGTGCT TTCCAACCTG 
ACTGACCCCA AAGCCAAAGA CAGCAAAGCA AAGGGAAAGG GGGCGAAACC AAAGTCTAAA 
CCAAAGAAAC AGGCAGTGGA AGACTCTTCA GCCACAGATC TGCTGTCCAG TAAACCGAGC 
TCAGATGTGG TGCCTTCAGC TTCCACAAAG GCTGGAAAAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_005161041.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161098.1 Danio rerio calpastatin (cast), transcript variant X19, mRNA.

Price & Availability↑

CloneID	ODa25618
Clone ID Related Accession (Same CDS sequence)	XM_005161041.4 , XM_005161041.3
Accession Version	XM_005161041.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	calpastatin isoform X19
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005161041.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCACA AAAAGAGATG TCATGGTCGT AAGAAAAACA AAAAGGAGAA TGGAGACACT 
TCATTTCAAA AACAAGAATA CGCTCCACAG ACATCAAGGT TCATGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGGT GCAGGTTGAA 
GTGGGTCCTT CAGCAGCAAA AGTCAGTGCA GATGCTGACG ATCCGTTTGA TGCCCTGTCT 
GGCACATTAC CATCATCACA ACCTTTGGCT CCTAAAGTCC ACTACACTGG ACCTGAGATC 
AAAGAGCCCA AACTGAAATC AGAGAAAGGT GTTTTGTGTG GTGAGCGAGA TGACACGCTG 
CCGCCCGGAT ACAGAAAGGA AGACCTGGAA AAGAAGCATG GAGCTGGAGT TCCAGCTGGA 
GTTCCGGAGA AGCCTAAAGA AGTTCCCAAG CCCATAAGCA CAGATGATGC ACTGGAATCT 
CTCTCCTCTA CGTTTGTGTC TCCAGCTCAA GCTGCTCCAA AGAAGACAGA CGTGAAAACT 
GAAACAATAG GAGCAGTGGA TGTTAAATCG GCAGGCACCA CTAACTTTGC TCCCCCTCCA 
CCATCTCAGC AGAAACAACC GGCGTCGTCT CAAGCTGCTG CTGTAACCAA ATCACCTGCT 
CCACCGGCAG ACAAGAAAGC TAAAATGGAG ACTCATGCAC AACCAGCCAA AGCAAAGACA 
GAGTCGGACA GCTCCATATC GCTGGACGCT CTCGATGCTT TGAGCGACAC ACTGGGGGCT 
TCTAAACCAC CCCAAAAATT ACCAGAACTC AAACCTGGAC AGATAGTTGA TGAGAAGAAA 
CAGACCTCAG AGAAAGGTGT TCGTGTTGGA GAAAGAGACG ACACACTTCC ACCACAATAC 
AGATTCAAAG AGGAAGATCT CAAGAAATAT CCTCCACCTG CGAAAGAGCC TTCACTGAAC 
ACAGATGATG CGATGGACAT ACTTTCAGGA GATTTCACGA CCCCTGCATC GGCACCTGTT 
GTCAAAGCTC CTGTTCCTCC TGCACAGGAC ACAAAGAAGC ATGCTGCAGT GCCTGAGAAG 
ACCAAAGATA TTCCCAAGAC TAAAGCTGAT GCTCTGTCAA GTGATTTCGT GGCTCCGGCA 
CATTCTGCTC CTAAGGTTTC TTCAGCTGCT CCTAAAACCA TCCCTCCAGG CCCACCACAA 
ACCCCAGATG AGGATCCTTT CAGTGCTTTG GGCGACACTC TTTCTGCCCC AGAACCACCA 
AAAAAACAAC CTGACCTGAA ACCTAAGGAC ATCGTACATG AGAAGGATGT GACGTCAAAG 
AAAGGAGTTC GTGTTGGAGA AAGAGACGAC ACACTCCCAC CAGAATACAG ATTCAAAGAG 
GAAGATCTCA AGAAATATCC TCCTCCTCAG AAAGATCCTT CCTTAGATGC TAATGAAGCT 
CTGGATATTC TGTCTGGGGA TTTTACAACT CCTTCCGCAC CATCGGCTGT TTCCCCTGCC 
TCTAAATCCT CTGCCAAGTC TTCAGATTCA GCTTTAGATG CTCTTGCTGG TGATTTCGTC 
GCTTCTTCTT CTGCGTCTTC AGTTCATTCT GCCGTCTCTG GCCCCCCACG CTCCGACAGA 
CTGTTGTCAG ACGGAACCTC ATCAGCTCTA GATGCCCTGT CAGAAACTCT GGGAGACATT 
AAAGCAGCCC CTGAACCCGC CCCTGTCCTA CCTAAAACTG TCGTTAAGGA GAAGACGGTC 
ATAGAAGAGA GAGTGAGTAA ACCGGGTGAG AGAGACGACT CCCTTCCACC AGATCAGCGG 
TTCACTGCGG AGGATCGCAA GGCATTTGAA GAAGCAAAGA AGAAAGACGC CAAACCAAAA 
CAGCCATCAA TGGACGATTC AGCGGCTCTG GACATGTTGT CTAGTGATTT TTCAGATGTA 
CCGACTGTAA AGCCCACTGT ATCTGATGGC AAAAGCTTCA CCCCTGAACC AACACCACCA 
ACCTTTAAGG CAACCGGTCC GGTTTTAGAC AAACTGGCAG ACAACGTGCT TTCCAACCTG 
ACTGACCCCA AAGCCAAAGA CAGCAAAGCA AAGGGAAAGG GGGCGAAACC AAAGTCTAAA 
CCAAAGAAAC AGGCAGTGGA AGACTCTTCA GCCACAGATC TGCTGTCCAG TAAACCGAGC 
TCAGATGTGG TGCCTTCAGC TTCCACAAAG GCTGGAAAAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161098.1
CDS	38..2242
Translation	MPHKKRCHGRKKNKKENGDTSFQKQEYAPQTSRFMSQQVTPTTTSQVSTVKPAQYEVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_005161041.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161041.3

ORF Insert Sequence:

ATGCCGCACA AAAAGAGATG TCATGGTCGT AAGAAAAACA AAAAGGAGAA TGGAGACACT 
TCATTTCAAA AACAAGAATA CGCTCCACAG ACATCAAGGT TCATGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGGT GCAGGTTGAA 
GTGGGTCCTT CAGCAGCAAA AGTCAGTGCA GATGCTGACG ATCCGTTTGA TGCCCTGTCT 
GGCACATTAC CATCATCACA ACCTTTGGCT CCTAAAGTCC ACTACACTGG ACCTGAGATC 
AAAGAGCCCA AACTGAAATC AGAGAAAGGT GTTTTGTGTG GTGAGCGAGA TGACACGCTG 
CCGCCCGGAT ACAGAAAGGA AGACCTGGAA AAGAAGCATG GAGCTGGAGT TCCAGCTGGA 
GTTCCGGAGA AGCCTAAAGA AGTTCCCAAG CCCATAAGCA CAGATGATGC ACTGGAATCT 
CTCTCCTCTA CGTTTGTGTC TCCAGCTCAA GCTGCTCCAA AGAAGACAGA CGTGAAAACT 
GAAACAATAG GAGCAGTGGA TGTTAAATCG GCAGGCACCA CTAACTTTGC TCCCCCTCCA 
CCATCTCAGC AGAAACAACC GGCGTCGTCT CAAGCTGCTG CTGTAACCAA ATCACCTGCT 
CCACCGGCAG ACAAGAAAGC TAAAATGGAG ACTCATGCAC AACCAGCCAA AGCAAAGACA 
GAGTCGGACA GCTCCATATC GCTGGACGCT CTCGATGCTT TGAGCGACAC ACTGGGGGCT 
TCTAAACCAC CCCAAAAATT ACCAGAACTC AAACCTGGAC AGATAGTTGA TGAGAAGAAA 
CAGACCTCAG AGAAAGGTGT TCGTGTTGGA GAAAGAGACG ACACACTTCC ACCACAATAC 
AGATTCAAAG AGGAAGATCT CAAGAAATAT CCTCCACCTG CGAAAGAGCC TTCACTGAAC 
ACAGATGATG CGATGGACAT ACTTTCAGGA GATTTCACGA CCCCTGCATC GGCACCTGTT 
GTCAAAGCTC CTGTTCCTCC TGCACAGGAC ACAAAGAAGC ATGCTGCAGT GCCTGAGAAG 
ACCAAAGATA TTCCCAAGAC TAAAGCTGAT GCTCTGTCAA GTGATTTCGT GGCTCCGGCA 
CATTCTGCTC CTAAGGTTTC TTCAGCTGCT CCTAAAACCA TCCCTCCAGG CCCACCACAA 
ACCCCAGATG AGGATCCTTT CAGTGCTTTG GGCGACACTC TTTCTGCCCC AGAACCACCA 
AAAAAACAAC CTGACCTGAA ACCTAAGGAC ATCGTACATG AGAAGGATGT GACGTCAAAG 
AAAGGAGTTC GTGTTGGAGA AAGAGACGAC ACACTCCCAC CAGAATACAG ATTCAAAGAG 
GAAGATCTCA AGAAATATCC TCCTCCTCAG AAAGATCCTT CCTTAGATGC TAATGAAGCT 
CTGGATATTC TGTCTGGGGA TTTTACAACT CCTTCCGCAC CATCGGCTGT TTCCCCTGCC 
TCTAAATCCT CTGCCAAGTC TTCAGATTCA GCTTTAGATG CTCTTGCTGG TGATTTCGTC 
GCTTCTTCTT CTGCGTCTTC AGTTCATTCT GCCGTCTCTG GCCCCCCACG CTCCGACAGA 
CTGTTGTCAG ACGGAACCTC ATCAGCTCTA GATGCCCTGT CAGAAACTCT GGGAGACATT 
AAAGCAGCCC CTGAACCCGC CCCTGTCCTA CCTAAAACTG TCGTTAAGGA GAAGACGGTC 
ATAGAAGAGA GAGTGAGTAA ACCGGGTGAG AGAGACGACT CCCTTCCACC AGATCAGCGG 
TTCACTGCGG AGGATCGCAA GGCATTTGAA GAAGCAAAGA AGAAAGACGC CAAACCAAAA 
CAGCCATCAA TGGACGATTC AGCGGCTCTG GACATGTTGT CTAGTGATTT TTCAGATGTA 
CCGACTGTAA AGCCCACTGT ATCTGATGGC AAAAGCTTCA CCCCTGAACC AACACCACCA 
ACCTTTAAGG CAACCGGTCC GGTTTTAGAC AAACTGGCAG ACAACGTGCT TTCCAACCTG 
ACTGACCCCA AAGCCAAAGA CAGCAAAGCA AAGGGAAAGG GGGCGAAACC AAAGTCTAAA 
CCAAAGAAAC AGGCAGTGGA AGACTCTTCA GCCACAGATC TGCTGTCCAG TAAACCGAGC 
TCAGATGTGG TGCCTTCAGC TTCCACAAAG GCTGGAAAAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_005161042.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161099.1 Danio rerio calpastatin (cast), transcript variant X20, mRNA.

Price & Availability↑

CloneID	ODa25619
Clone ID Related Accession (Same CDS sequence)	XM_005161042.3 , XM_005161043.4 , XM_005161043.3 , XM_005161042.4
Accession Version	XM_005161042.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	calpastatin isoform X20
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005161042.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC AGGTCACACC CACCACAACA TCTCAGGTCT CCACAGTGAA ACCTGCACAA 
TATGAGAAAG GAGCTACACA ATCGTCTACA GCAGCTGCGA CTGTCAAATC CGGCGCTGGA 
GATCCTGCAA CCACCCAGAA AGGATCTTCT CAAGTCAAAC CTCAGGTGCA GGTTGAAGTG 
GGTCCTTCAG CAGCAAAAGT CAGTGCAGAT GCTGACGATC CGTTTGATGC CCTGTCTGGC 
ACATTACCAT CATCACAACC TTTGGCTCCT AAAGTCCACT ACACTGGACC TGAGATCAAA 
GAGCCCAAAC TGAAATCAGA GAAAGGTGTT TTGTGTGGTG AGCGAGATGA CACGCTGCCG 
CCCGGATACA GAAAGGAAGA CCTGGAAAAG AAGCATGGAG CTGGAGTTCC AGCTGGAGTT 
CCGGAGAAGC CTAAAGAAGT TCCCAAGCCC ATAAGCACAG ATGATGCACT GGAATCTCTC 
TCCTCTACGT TTGTGTCTCC AGCTCAAGCT GCTCCAAAGA AGACAGACGT GAAAACTGAA 
ACAATAGGAG CAGTGGATGT TAAATCGGCA GGCACCACTA ACTTTGCTCC CCCTCCACCA 
TCTCAGCAGA AACAACCGGC GTCGTCTCAA GCTGCTGCTG TAACCAAATC ACCTGCTCCA 
CCGGCAGACA AGAAAGCTAA AATGGAGACT CATGCACAAC CAGCCAAAGC AAAGACAGAG 
TCGGACAGCT CCATATCGCT GGACGCTCTC GATGCTTTGA GCGACACACT GGGGGCTTCT 
AAACCACCCC AAAAATTACC AGAACTCAAA CCTGGACAGA TAGTTGATGA GAAGAAACAG 
ACCTCAGAGA AAGGTGTTCG TGTTGGAGAA AGAGACGACA CACTTCCACC ACAATACAGA 
TTCAAAGAGG AAGATCTCAA GAAATATCCT CCACCTGCGA AAGAGCCTTC ACTGAACACA 
GATGATGCGA TGGACATACT TTCAGGAGAT TTCACGACCC CTGCATCGGC ACCTGTTGTC 
AAAGCTCCTG TTCCTCCTGC ACAGGACACA AAGAAGCATG CTGCAGTGCC TGAGAAGACC 
AAAGATATTC CCAAGACTAA AGCTGATGCT CTGTCAAGTG ATTTCGTGGC TCCGGCACAT 
TCTGCTCCTA AGGTTTCTTC AGCTGCTCCT AAAACCATCC CTCCAGGCCC ACCACAAACC 
CCAGATGAGG ATCCTTTCAG TGCTTTGGGC GACACTCTTT CTGCCCCAGA ACCACCAAAA 
AAACAACCTG ACCTGAAACC TAAGGACATC GTACATGAGA AGGATGTGAC GTCAAAGAAA 
GGAGTTCGTG TTGGAGAAAG AGACGACACA CTCCCACCAG AATACAGATT CAAAGAGGAA 
GATCTCAAGA AATATCCTCC TCCTCAGAAA GATCCTTCCT TAGATGCTAA TGAAGCTCTG 
GATATTCTGT CTGGGGATTT TACAACTCCT TCCGCACCAT CGGCTGTTTC CCCTGCCTCT 
AAATCCTCTG CCAAGTCTTC AGATTCAGCT TTAGATGCTC TTGCTGGTGA TTTCGTCGCT 
TCTTCTTCTG CGTCTTCAGT TCATTCTGCC GTCTCTGGCC CCCCACGCTC CGACAGACTG 
TTGTCAGACG GAACCTCATC AGCTCTAGAT GCCCTGTCAG AAACTCTGGG AGACATTAAA 
GCAGCCCCTG AACCCGCCCC TGTCCTACCT AAAACTGTCG TTAAGGAGAA GACGGTCATA 
GAAGAGAGAG TGAGTAAACC GGGTGAGAGA GACGACTCCC TTCCACCAGA TCAGCGGTTC 
ACTGCGGAGG ATCGCAAGGC ATTTGAAGAA GCAAAGAAGA AAGACGCCAA ACCAAAACAG 
CCATCAATGG ACGATTCAGC GGCTCTGGAC ATGTTGTCTA GTGATTTTTC AGATGTACCG 
ACTGTAAAGC CCACTGTATC TGATGGCAAA AGCTTCACCC CTGAACCAAC ACCACCAACC 
TTTAAGGCAA CCGGTCCGGT TTTAGACAAA CTGGCAGACA ACGTGCTTTC CAACCTGACT 
GACCCCAAAG CCAAAGACAG CAAAGCAAAG GGAAAGGGGG CGAAACCAAA GTCTAAACCA 
AAGAAACAGG CAGTGGAAGA CTCTTCAGCC ACAGATCTGC TGTCCAGTAA ACCGAGCTCA 
GATGTGGTGC CTTCAGCTTC CACAAAGGCT GGAAAAAGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161099.1
CDS	129..2330
Translation	MSQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_005161042.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161042.3

ORF Insert Sequence:

ATGTCTCAGC AGGTCACACC CACCACAACA TCTCAGGTCT CCACAGTGAA ACCTGCACAA 
TATGAGAAAG GAGCTACACA ATCGTCTACA GCAGCTGCGA CTGTCAAATC CGGCGCTGGA 
GATCCTGCAA CCACCCAGAA AGGATCTTCT CAAGTCAAAC CTCAGGTGCA GGTTGAAGTG 
GGTCCTTCAG CAGCAAAAGT CAGTGCAGAT GCTGACGATC CGTTTGATGC CCTGTCTGGC 
ACATTACCAT CATCACAACC TTTGGCTCCT AAAGTCCACT ACACTGGACC TGAGATCAAA 
GAGCCCAAAC TGAAATCAGA GAAAGGTGTT TTGTGTGGTG AGCGAGATGA CACGCTGCCG 
CCCGGATACA GAAAGGAAGA CCTGGAAAAG AAGCATGGAG CTGGAGTTCC AGCTGGAGTT 
CCGGAGAAGC CTAAAGAAGT TCCCAAGCCC ATAAGCACAG ATGATGCACT GGAATCTCTC 
TCCTCTACGT TTGTGTCTCC AGCTCAAGCT GCTCCAAAGA AGACAGACGT GAAAACTGAA 
ACAATAGGAG CAGTGGATGT TAAATCGGCA GGCACCACTA ACTTTGCTCC CCCTCCACCA 
TCTCAGCAGA AACAACCGGC GTCGTCTCAA GCTGCTGCTG TAACCAAATC ACCTGCTCCA 
CCGGCAGACA AGAAAGCTAA AATGGAGACT CATGCACAAC CAGCCAAAGC AAAGACAGAG 
TCGGACAGCT CCATATCGCT GGACGCTCTC GATGCTTTGA GCGACACACT GGGGGCTTCT 
AAACCACCCC AAAAATTACC AGAACTCAAA CCTGGACAGA TAGTTGATGA GAAGAAACAG 
ACCTCAGAGA AAGGTGTTCG TGTTGGAGAA AGAGACGACA CACTTCCACC ACAATACAGA 
TTCAAAGAGG AAGATCTCAA GAAATATCCT CCACCTGCGA AAGAGCCTTC ACTGAACACA 
GATGATGCGA TGGACATACT TTCAGGAGAT TTCACGACCC CTGCATCGGC ACCTGTTGTC 
AAAGCTCCTG TTCCTCCTGC ACAGGACACA AAGAAGCATG CTGCAGTGCC TGAGAAGACC 
AAAGATATTC CCAAGACTAA AGCTGATGCT CTGTCAAGTG ATTTCGTGGC TCCGGCACAT 
TCTGCTCCTA AGGTTTCTTC AGCTGCTCCT AAAACCATCC CTCCAGGCCC ACCACAAACC 
CCAGATGAGG ATCCTTTCAG TGCTTTGGGC GACACTCTTT CTGCCCCAGA ACCACCAAAA 
AAACAACCTG ACCTGAAACC TAAGGACATC GTACATGAGA AGGATGTGAC GTCAAAGAAA 
GGAGTTCGTG TTGGAGAAAG AGACGACACA CTCCCACCAG AATACAGATT CAAAGAGGAA 
GATCTCAAGA AATATCCTCC TCCTCAGAAA GATCCTTCCT TAGATGCTAA TGAAGCTCTG 
GATATTCTGT CTGGGGATTT TACAACTCCT TCCGCACCAT CGGCTGTTTC CCCTGCCTCT 
AAATCCTCTG CCAAGTCTTC AGATTCAGCT TTAGATGCTC TTGCTGGTGA TTTCGTCGCT 
TCTTCTTCTG CGTCTTCAGT TCATTCTGCC GTCTCTGGCC CCCCACGCTC CGACAGACTG 
TTGTCAGACG GAACCTCATC AGCTCTAGAT GCCCTGTCAG AAACTCTGGG AGACATTAAA 
GCAGCCCCTG AACCCGCCCC TGTCCTACCT AAAACTGTCG TTAAGGAGAA GACGGTCATA 
GAAGAGAGAG TGAGTAAACC GGGTGAGAGA GACGACTCCC TTCCACCAGA TCAGCGGTTC 
ACTGCGGAGG ATCGCAAGGC ATTTGAAGAA GCAAAGAAGA AAGACGCCAA ACCAAAACAG 
CCATCAATGG ACGATTCAGC GGCTCTGGAC ATGTTGTCTA GTGATTTTTC AGATGTACCG 
ACTGTAAAGC CCACTGTATC TGATGGCAAA AGCTTCACCC CTGAACCAAC ACCACCAACC 
TTTAAGGCAA CCGGTCCGGT TTTAGACAAA CTGGCAGACA ACGTGCTTTC CAACCTGACT 
GACCCCAAAG CCAAAGACAG CAAAGCAAAG GGAAAGGGGG CGAAACCAAA GTCTAAACCA 
AAGAAACAGG CAGTGGAAGA CTCTTCAGCC ACAGATCTGC TGTCCAGTAA ACCGAGCTCA 
GATGTGGTGC CTTCAGCTTC CACAAAGGCT GGAAAAAGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_005161043.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161100.1 Danio rerio calpastatin (cast), transcript variant X23, mRNA.

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CloneID	ODa25619
Clone ID Related Accession (Same CDS sequence)	XM_005161042.3 , XM_005161043.4 , XM_005161043.3 , XM_005161042.4
Accession Version	XM_005161043.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calpastatin isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005161043.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC AGGTCACACC CACCACAACA TCTCAGGTCT CCACAGTGAA ACCTGCACAA 
TATGAGAAAG GAGCTACACA ATCGTCTACA GCAGCTGCGA CTGTCAAATC CGGCGCTGGA 
GATCCTGCAA CCACCCAGAA AGGATCTTCT CAAGTCAAAC CTCAGGTGCA GGTTGAAGTG 
GGTCCTTCAG CAGCAAAAGT CAGTGCAGAT GCTGACGATC CGTTTGATGC CCTGTCTGGC 
ACATTACCAT CATCACAACC TTTGGCTCCT AAAGTCCACT ACACTGGACC TGAGATCAAA 
GAGCCCAAAC TGAAATCAGA GAAAGGTGTT TTGTGTGGTG AGCGAGATGA CACGCTGCCG 
CCCGGATACA GAAAGGAAGA CCTGGAAAAG AAGCATGGAG CTGGAGTTCC AGCTGGAGTT 
CCGGAGAAGC CTAAAGAAGT TCCCAAGCCC ATAAGCACAG ATGATGCACT GGAATCTCTC 
TCCTCTACGT TTGTGTCTCC AGCTCAAGCT GCTCCAAAGA AGACAGACGT GAAAACTGAA 
ACAATAGGAG CAGTGGATGT TAAATCGGCA GGCACCACTA ACTTTGCTCC CCCTCCACCA 
TCTCAGCAGA AACAACCGGC GTCGTCTCAA GCTGCTGCTG TAACCAAATC ACCTGCTCCA 
CCGGCAGACA AGAAAGCTAA AATGGAGACT CATGCACAAC CAGCCAAAGC AAAGACAGAG 
TCGGACAGCT CCATATCGCT GGACGCTCTC GATGCTTTGA GCGACACACT GGGGGCTTCT 
AAACCACCCC AAAAATTACC AGAACTCAAA CCTGGACAGA TAGTTGATGA GAAGAAACAG 
ACCTCAGAGA AAGGTGTTCG TGTTGGAGAA AGAGACGACA CACTTCCACC ACAATACAGA 
TTCAAAGAGG AAGATCTCAA GAAATATCCT CCACCTGCGA AAGAGCCTTC ACTGAACACA 
GATGATGCGA TGGACATACT TTCAGGAGAT TTCACGACCC CTGCATCGGC ACCTGTTGTC 
AAAGCTCCTG TTCCTCCTGC ACAGGACACA AAGAAGCATG CTGCAGTGCC TGAGAAGACC 
AAAGATATTC CCAAGACTAA AGCTGATGCT CTGTCAAGTG ATTTCGTGGC TCCGGCACAT 
TCTGCTCCTA AGGTTTCTTC AGCTGCTCCT AAAACCATCC CTCCAGGCCC ACCACAAACC 
CCAGATGAGG ATCCTTTCAG TGCTTTGGGC GACACTCTTT CTGCCCCAGA ACCACCAAAA 
AAACAACCTG ACCTGAAACC TAAGGACATC GTACATGAGA AGGATGTGAC GTCAAAGAAA 
GGAGTTCGTG TTGGAGAAAG AGACGACACA CTCCCACCAG AATACAGATT CAAAGAGGAA 
GATCTCAAGA AATATCCTCC TCCTCAGAAA GATCCTTCCT TAGATGCTAA TGAAGCTCTG 
GATATTCTGT CTGGGGATTT TACAACTCCT TCCGCACCAT CGGCTGTTTC CCCTGCCTCT 
AAATCCTCTG CCAAGTCTTC AGATTCAGCT TTAGATGCTC TTGCTGGTGA TTTCGTCGCT 
TCTTCTTCTG CGTCTTCAGT TCATTCTGCC GTCTCTGGCC CCCCACGCTC CGACAGACTG 
TTGTCAGACG GAACCTCATC AGCTCTAGAT GCCCTGTCAG AAACTCTGGG AGACATTAAA 
GCAGCCCCTG AACCCGCCCC TGTCCTACCT AAAACTGTCG TTAAGGAGAA GACGGTCATA 
GAAGAGAGAG TGAGTAAACC GGGTGAGAGA GACGACTCCC TTCCACCAGA TCAGCGGTTC 
ACTGCGGAGG ATCGCAAGGC ATTTGAAGAA GCAAAGAAGA AAGACGCCAA ACCAAAACAG 
CCATCAATGG ACGATTCAGC GGCTCTGGAC ATGTTGTCTA GTGATTTTTC AGATGTACCG 
ACTGTAAAGC CCACTGTATC TGATGGCAAA AGCTTCACCC CTGAACCAAC ACCACCAACC 
TTTAAGGCAA CCGGTCCGGT TTTAGACAAA CTGGCAGACA ACGTGCTTTC CAACCTGACT 
GACCCCAAAG CCAAAGACAG CAAAGCAAAG GGAAAGGGGG CGAAACCAAA GTCTAAACCA 
AAGAAACAGG CAGTGGAAGA CTCTTCAGCC ACAGATCTGC TGTCCAGTAA ACCGAGCTCA 
GATGTGGTGC CTTCAGCTTC CACAAAGGCT GGAAAAAGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161100.1
CDS	94..2295
Translation	MSQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_005161043.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161043.4

ORF Insert Sequence:

ATGTCTCAGC AGGTCACACC CACCACAACA TCTCAGGTCT CCACAGTGAA ACCTGCACAA 
TATGAGAAAG GAGCTACACA ATCGTCTACA GCAGCTGCGA CTGTCAAATC CGGCGCTGGA 
GATCCTGCAA CCACCCAGAA AGGATCTTCT CAAGTCAAAC CTCAGGTGCA GGTTGAAGTG 
GGTCCTTCAG CAGCAAAAGT CAGTGCAGAT GCTGACGATC CGTTTGATGC CCTGTCTGGC 
ACATTACCAT CATCACAACC TTTGGCTCCT AAAGTCCACT ACACTGGACC TGAGATCAAA 
GAGCCCAAAC TGAAATCAGA GAAAGGTGTT TTGTGTGGTG AGCGAGATGA CACGCTGCCG 
CCCGGATACA GAAAGGAAGA CCTGGAAAAG AAGCATGGAG CTGGAGTTCC AGCTGGAGTT 
CCGGAGAAGC CTAAAGAAGT TCCCAAGCCC ATAAGCACAG ATGATGCACT GGAATCTCTC 
TCCTCTACGT TTGTGTCTCC AGCTCAAGCT GCTCCAAAGA AGACAGACGT GAAAACTGAA 
ACAATAGGAG CAGTGGATGT TAAATCGGCA GGCACCACTA ACTTTGCTCC CCCTCCACCA 
TCTCAGCAGA AACAACCGGC GTCGTCTCAA GCTGCTGCTG TAACCAAATC ACCTGCTCCA 
CCGGCAGACA AGAAAGCTAA AATGGAGACT CATGCACAAC CAGCCAAAGC AAAGACAGAG 
TCGGACAGCT CCATATCGCT GGACGCTCTC GATGCTTTGA GCGACACACT GGGGGCTTCT 
AAACCACCCC AAAAATTACC AGAACTCAAA CCTGGACAGA TAGTTGATGA GAAGAAACAG 
ACCTCAGAGA AAGGTGTTCG TGTTGGAGAA AGAGACGACA CACTTCCACC ACAATACAGA 
TTCAAAGAGG AAGATCTCAA GAAATATCCT CCACCTGCGA AAGAGCCTTC ACTGAACACA 
GATGATGCGA TGGACATACT TTCAGGAGAT TTCACGACCC CTGCATCGGC ACCTGTTGTC 
AAAGCTCCTG TTCCTCCTGC ACAGGACACA AAGAAGCATG CTGCAGTGCC TGAGAAGACC 
AAAGATATTC CCAAGACTAA AGCTGATGCT CTGTCAAGTG ATTTCGTGGC TCCGGCACAT 
TCTGCTCCTA AGGTTTCTTC AGCTGCTCCT AAAACCATCC CTCCAGGCCC ACCACAAACC 
CCAGATGAGG ATCCTTTCAG TGCTTTGGGC GACACTCTTT CTGCCCCAGA ACCACCAAAA 
AAACAACCTG ACCTGAAACC TAAGGACATC GTACATGAGA AGGATGTGAC GTCAAAGAAA 
GGAGTTCGTG TTGGAGAAAG AGACGACACA CTCCCACCAG AATACAGATT CAAAGAGGAA 
GATCTCAAGA AATATCCTCC TCCTCAGAAA GATCCTTCCT TAGATGCTAA TGAAGCTCTG 
GATATTCTGT CTGGGGATTT TACAACTCCT TCCGCACCAT CGGCTGTTTC CCCTGCCTCT 
AAATCCTCTG CCAAGTCTTC AGATTCAGCT TTAGATGCTC TTGCTGGTGA TTTCGTCGCT 
TCTTCTTCTG CGTCTTCAGT TCATTCTGCC GTCTCTGGCC CCCCACGCTC CGACAGACTG 
TTGTCAGACG GAACCTCATC AGCTCTAGAT GCCCTGTCAG AAACTCTGGG AGACATTAAA 
GCAGCCCCTG AACCCGCCCC TGTCCTACCT AAAACTGTCG TTAAGGAGAA GACGGTCATA 
GAAGAGAGAG TGAGTAAACC GGGTGAGAGA GACGACTCCC TTCCACCAGA TCAGCGGTTC 
ACTGCGGAGG ATCGCAAGGC ATTTGAAGAA GCAAAGAAGA AAGACGCCAA ACCAAAACAG 
CCATCAATGG ACGATTCAGC GGCTCTGGAC ATGTTGTCTA GTGATTTTTC AGATGTACCG 
ACTGTAAAGC CCACTGTATC TGATGGCAAA AGCTTCACCC CTGAACCAAC ACCACCAACC 
TTTAAGGCAA CCGGTCCGGT TTTAGACAAA CTGGCAGACA ACGTGCTTTC CAACCTGACT 
GACCCCAAAG CCAAAGACAG CAAAGCAAAG GGAAAGGGGG CGAAACCAAA GTCTAAACCA 
AAGAAACAGG CAGTGGAAGA CTCTTCAGCC ACAGATCTGC TGTCCAGTAA ACCGAGCTCA 
GATGTGGTGC CTTCAGCTTC CACAAAGGCT GGAAAAAGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_005161043.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161100.1 Danio rerio calpastatin (cast), transcript variant X21, mRNA.

Price & Availability↑

CloneID	ODa25619
Clone ID Related Accession (Same CDS sequence)	XM_005161042.3 , XM_005161043.4 , XM_005161043.3 , XM_005161042.4
Accession Version	XM_005161043.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	calpastatin isoform X21
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005161043.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC AGGTCACACC CACCACAACA TCTCAGGTCT CCACAGTGAA ACCTGCACAA 
TATGAGAAAG GAGCTACACA ATCGTCTACA GCAGCTGCGA CTGTCAAATC CGGCGCTGGA 
GATCCTGCAA CCACCCAGAA AGGATCTTCT CAAGTCAAAC CTCAGGTGCA GGTTGAAGTG 
GGTCCTTCAG CAGCAAAAGT CAGTGCAGAT GCTGACGATC CGTTTGATGC CCTGTCTGGC 
ACATTACCAT CATCACAACC TTTGGCTCCT AAAGTCCACT ACACTGGACC TGAGATCAAA 
GAGCCCAAAC TGAAATCAGA GAAAGGTGTT TTGTGTGGTG AGCGAGATGA CACGCTGCCG 
CCCGGATACA GAAAGGAAGA CCTGGAAAAG AAGCATGGAG CTGGAGTTCC AGCTGGAGTT 
CCGGAGAAGC CTAAAGAAGT TCCCAAGCCC ATAAGCACAG ATGATGCACT GGAATCTCTC 
TCCTCTACGT TTGTGTCTCC AGCTCAAGCT GCTCCAAAGA AGACAGACGT GAAAACTGAA 
ACAATAGGAG CAGTGGATGT TAAATCGGCA GGCACCACTA ACTTTGCTCC CCCTCCACCA 
TCTCAGCAGA AACAACCGGC GTCGTCTCAA GCTGCTGCTG TAACCAAATC ACCTGCTCCA 
CCGGCAGACA AGAAAGCTAA AATGGAGACT CATGCACAAC CAGCCAAAGC AAAGACAGAG 
TCGGACAGCT CCATATCGCT GGACGCTCTC GATGCTTTGA GCGACACACT GGGGGCTTCT 
AAACCACCCC AAAAATTACC AGAACTCAAA CCTGGACAGA TAGTTGATGA GAAGAAACAG 
ACCTCAGAGA AAGGTGTTCG TGTTGGAGAA AGAGACGACA CACTTCCACC ACAATACAGA 
TTCAAAGAGG AAGATCTCAA GAAATATCCT CCACCTGCGA AAGAGCCTTC ACTGAACACA 
GATGATGCGA TGGACATACT TTCAGGAGAT TTCACGACCC CTGCATCGGC ACCTGTTGTC 
AAAGCTCCTG TTCCTCCTGC ACAGGACACA AAGAAGCATG CTGCAGTGCC TGAGAAGACC 
AAAGATATTC CCAAGACTAA AGCTGATGCT CTGTCAAGTG ATTTCGTGGC TCCGGCACAT 
TCTGCTCCTA AGGTTTCTTC AGCTGCTCCT AAAACCATCC CTCCAGGCCC ACCACAAACC 
CCAGATGAGG ATCCTTTCAG TGCTTTGGGC GACACTCTTT CTGCCCCAGA ACCACCAAAA 
AAACAACCTG ACCTGAAACC TAAGGACATC GTACATGAGA AGGATGTGAC GTCAAAGAAA 
GGAGTTCGTG TTGGAGAAAG AGACGACACA CTCCCACCAG AATACAGATT CAAAGAGGAA 
GATCTCAAGA AATATCCTCC TCCTCAGAAA GATCCTTCCT TAGATGCTAA TGAAGCTCTG 
GATATTCTGT CTGGGGATTT TACAACTCCT TCCGCACCAT CGGCTGTTTC CCCTGCCTCT 
AAATCCTCTG CCAAGTCTTC AGATTCAGCT TTAGATGCTC TTGCTGGTGA TTTCGTCGCT 
TCTTCTTCTG CGTCTTCAGT TCATTCTGCC GTCTCTGGCC CCCCACGCTC CGACAGACTG 
TTGTCAGACG GAACCTCATC AGCTCTAGAT GCCCTGTCAG AAACTCTGGG AGACATTAAA 
GCAGCCCCTG AACCCGCCCC TGTCCTACCT AAAACTGTCG TTAAGGAGAA GACGGTCATA 
GAAGAGAGAG TGAGTAAACC GGGTGAGAGA GACGACTCCC TTCCACCAGA TCAGCGGTTC 
ACTGCGGAGG ATCGCAAGGC ATTTGAAGAA GCAAAGAAGA AAGACGCCAA ACCAAAACAG 
CCATCAATGG ACGATTCAGC GGCTCTGGAC ATGTTGTCTA GTGATTTTTC AGATGTACCG 
ACTGTAAAGC CCACTGTATC TGATGGCAAA AGCTTCACCC CTGAACCAAC ACCACCAACC 
TTTAAGGCAA CCGGTCCGGT TTTAGACAAA CTGGCAGACA ACGTGCTTTC CAACCTGACT 
GACCCCAAAG CCAAAGACAG CAAAGCAAAG GGAAAGGGGG CGAAACCAAA GTCTAAACCA 
AAGAAACAGG CAGTGGAAGA CTCTTCAGCC ACAGATCTGC TGTCCAGTAA ACCGAGCTCA 
GATGTGGTGC CTTCAGCTTC CACAAAGGCT GGAAAAAGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161100.1
CDS	230..2431
Translation	MSQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_005161043.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161043.3

ORF Insert Sequence:

ATGTCTCAGC AGGTCACACC CACCACAACA TCTCAGGTCT CCACAGTGAA ACCTGCACAA 
TATGAGAAAG GAGCTACACA ATCGTCTACA GCAGCTGCGA CTGTCAAATC CGGCGCTGGA 
GATCCTGCAA CCACCCAGAA AGGATCTTCT CAAGTCAAAC CTCAGGTGCA GGTTGAAGTG 
GGTCCTTCAG CAGCAAAAGT CAGTGCAGAT GCTGACGATC CGTTTGATGC CCTGTCTGGC 
ACATTACCAT CATCACAACC TTTGGCTCCT AAAGTCCACT ACACTGGACC TGAGATCAAA 
GAGCCCAAAC TGAAATCAGA GAAAGGTGTT TTGTGTGGTG AGCGAGATGA CACGCTGCCG 
CCCGGATACA GAAAGGAAGA CCTGGAAAAG AAGCATGGAG CTGGAGTTCC AGCTGGAGTT 
CCGGAGAAGC CTAAAGAAGT TCCCAAGCCC ATAAGCACAG ATGATGCACT GGAATCTCTC 
TCCTCTACGT TTGTGTCTCC AGCTCAAGCT GCTCCAAAGA AGACAGACGT GAAAACTGAA 
ACAATAGGAG CAGTGGATGT TAAATCGGCA GGCACCACTA ACTTTGCTCC CCCTCCACCA 
TCTCAGCAGA AACAACCGGC GTCGTCTCAA GCTGCTGCTG TAACCAAATC ACCTGCTCCA 
CCGGCAGACA AGAAAGCTAA AATGGAGACT CATGCACAAC CAGCCAAAGC AAAGACAGAG 
TCGGACAGCT CCATATCGCT GGACGCTCTC GATGCTTTGA GCGACACACT GGGGGCTTCT 
AAACCACCCC AAAAATTACC AGAACTCAAA CCTGGACAGA TAGTTGATGA GAAGAAACAG 
ACCTCAGAGA AAGGTGTTCG TGTTGGAGAA AGAGACGACA CACTTCCACC ACAATACAGA 
TTCAAAGAGG AAGATCTCAA GAAATATCCT CCACCTGCGA AAGAGCCTTC ACTGAACACA 
GATGATGCGA TGGACATACT TTCAGGAGAT TTCACGACCC CTGCATCGGC ACCTGTTGTC 
AAAGCTCCTG TTCCTCCTGC ACAGGACACA AAGAAGCATG CTGCAGTGCC TGAGAAGACC 
AAAGATATTC CCAAGACTAA AGCTGATGCT CTGTCAAGTG ATTTCGTGGC TCCGGCACAT 
TCTGCTCCTA AGGTTTCTTC AGCTGCTCCT AAAACCATCC CTCCAGGCCC ACCACAAACC 
CCAGATGAGG ATCCTTTCAG TGCTTTGGGC GACACTCTTT CTGCCCCAGA ACCACCAAAA 
AAACAACCTG ACCTGAAACC TAAGGACATC GTACATGAGA AGGATGTGAC GTCAAAGAAA 
GGAGTTCGTG TTGGAGAAAG AGACGACACA CTCCCACCAG AATACAGATT CAAAGAGGAA 
GATCTCAAGA AATATCCTCC TCCTCAGAAA GATCCTTCCT TAGATGCTAA TGAAGCTCTG 
GATATTCTGT CTGGGGATTT TACAACTCCT TCCGCACCAT CGGCTGTTTC CCCTGCCTCT 
AAATCCTCTG CCAAGTCTTC AGATTCAGCT TTAGATGCTC TTGCTGGTGA TTTCGTCGCT 
TCTTCTTCTG CGTCTTCAGT TCATTCTGCC GTCTCTGGCC CCCCACGCTC CGACAGACTG 
TTGTCAGACG GAACCTCATC AGCTCTAGAT GCCCTGTCAG AAACTCTGGG AGACATTAAA 
GCAGCCCCTG AACCCGCCCC TGTCCTACCT AAAACTGTCG TTAAGGAGAA GACGGTCATA 
GAAGAGAGAG TGAGTAAACC GGGTGAGAGA GACGACTCCC TTCCACCAGA TCAGCGGTTC 
ACTGCGGAGG ATCGCAAGGC ATTTGAAGAA GCAAAGAAGA AAGACGCCAA ACCAAAACAG 
CCATCAATGG ACGATTCAGC GGCTCTGGAC ATGTTGTCTA GTGATTTTTC AGATGTACCG 
ACTGTAAAGC CCACTGTATC TGATGGCAAA AGCTTCACCC CTGAACCAAC ACCACCAACC 
TTTAAGGCAA CCGGTCCGGT TTTAGACAAA CTGGCAGACA ACGTGCTTTC CAACCTGACT 
GACCCCAAAG CCAAAGACAG CAAAGCAAAG GGAAAGGGGG CGAAACCAAA GTCTAAACCA 
AAGAAACAGG CAGTGGAAGA CTCTTCAGCC ACAGATCTGC TGTCCAGTAA ACCGAGCTCA 
GATGTGGTGC CTTCAGCTTC CACAAAGGCT GGAAAAAGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_005161042.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161099.1 Danio rerio calpastatin (cast), transcript variant X22, mRNA.

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CloneID	ODa25619
Clone ID Related Accession (Same CDS sequence)	XM_005161042.3 , XM_005161043.4 , XM_005161043.3 , XM_005161042.4
Accession Version	XM_005161042.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calpastatin isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005161042.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC AGGTCACACC CACCACAACA TCTCAGGTCT CCACAGTGAA ACCTGCACAA 
TATGAGAAAG GAGCTACACA ATCGTCTACA GCAGCTGCGA CTGTCAAATC CGGCGCTGGA 
GATCCTGCAA CCACCCAGAA AGGATCTTCT CAAGTCAAAC CTCAGGTGCA GGTTGAAGTG 
GGTCCTTCAG CAGCAAAAGT CAGTGCAGAT GCTGACGATC CGTTTGATGC CCTGTCTGGC 
ACATTACCAT CATCACAACC TTTGGCTCCT AAAGTCCACT ACACTGGACC TGAGATCAAA 
GAGCCCAAAC TGAAATCAGA GAAAGGTGTT TTGTGTGGTG AGCGAGATGA CACGCTGCCG 
CCCGGATACA GAAAGGAAGA CCTGGAAAAG AAGCATGGAG CTGGAGTTCC AGCTGGAGTT 
CCGGAGAAGC CTAAAGAAGT TCCCAAGCCC ATAAGCACAG ATGATGCACT GGAATCTCTC 
TCCTCTACGT TTGTGTCTCC AGCTCAAGCT GCTCCAAAGA AGACAGACGT GAAAACTGAA 
ACAATAGGAG CAGTGGATGT TAAATCGGCA GGCACCACTA ACTTTGCTCC CCCTCCACCA 
TCTCAGCAGA AACAACCGGC GTCGTCTCAA GCTGCTGCTG TAACCAAATC ACCTGCTCCA 
CCGGCAGACA AGAAAGCTAA AATGGAGACT CATGCACAAC CAGCCAAAGC AAAGACAGAG 
TCGGACAGCT CCATATCGCT GGACGCTCTC GATGCTTTGA GCGACACACT GGGGGCTTCT 
AAACCACCCC AAAAATTACC AGAACTCAAA CCTGGACAGA TAGTTGATGA GAAGAAACAG 
ACCTCAGAGA AAGGTGTTCG TGTTGGAGAA AGAGACGACA CACTTCCACC ACAATACAGA 
TTCAAAGAGG AAGATCTCAA GAAATATCCT CCACCTGCGA AAGAGCCTTC ACTGAACACA 
GATGATGCGA TGGACATACT TTCAGGAGAT TTCACGACCC CTGCATCGGC ACCTGTTGTC 
AAAGCTCCTG TTCCTCCTGC ACAGGACACA AAGAAGCATG CTGCAGTGCC TGAGAAGACC 
AAAGATATTC CCAAGACTAA AGCTGATGCT CTGTCAAGTG ATTTCGTGGC TCCGGCACAT 
TCTGCTCCTA AGGTTTCTTC AGCTGCTCCT AAAACCATCC CTCCAGGCCC ACCACAAACC 
CCAGATGAGG ATCCTTTCAG TGCTTTGGGC GACACTCTTT CTGCCCCAGA ACCACCAAAA 
AAACAACCTG ACCTGAAACC TAAGGACATC GTACATGAGA AGGATGTGAC GTCAAAGAAA 
GGAGTTCGTG TTGGAGAAAG AGACGACACA CTCCCACCAG AATACAGATT CAAAGAGGAA 
GATCTCAAGA AATATCCTCC TCCTCAGAAA GATCCTTCCT TAGATGCTAA TGAAGCTCTG 
GATATTCTGT CTGGGGATTT TACAACTCCT TCCGCACCAT CGGCTGTTTC CCCTGCCTCT 
AAATCCTCTG CCAAGTCTTC AGATTCAGCT TTAGATGCTC TTGCTGGTGA TTTCGTCGCT 
TCTTCTTCTG CGTCTTCAGT TCATTCTGCC GTCTCTGGCC CCCCACGCTC CGACAGACTG 
TTGTCAGACG GAACCTCATC AGCTCTAGAT GCCCTGTCAG AAACTCTGGG AGACATTAAA 
GCAGCCCCTG AACCCGCCCC TGTCCTACCT AAAACTGTCG TTAAGGAGAA GACGGTCATA 
GAAGAGAGAG TGAGTAAACC GGGTGAGAGA GACGACTCCC TTCCACCAGA TCAGCGGTTC 
ACTGCGGAGG ATCGCAAGGC ATTTGAAGAA GCAAAGAAGA AAGACGCCAA ACCAAAACAG 
CCATCAATGG ACGATTCAGC GGCTCTGGAC ATGTTGTCTA GTGATTTTTC AGATGTACCG 
ACTGTAAAGC CCACTGTATC TGATGGCAAA AGCTTCACCC CTGAACCAAC ACCACCAACC 
TTTAAGGCAA CCGGTCCGGT TTTAGACAAA CTGGCAGACA ACGTGCTTTC CAACCTGACT 
GACCCCAAAG CCAAAGACAG CAAAGCAAAG GGAAAGGGGG CGAAACCAAA GTCTAAACCA 
AAGAAACAGG CAGTGGAAGA CTCTTCAGCC ACAGATCTGC TGTCCAGTAA ACCGAGCTCA 
GATGTGGTGC CTTCAGCTTC CACAAAGGCT GGAAAAAGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161099.1
CDS	149..2350
Translation	MSQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_005161042.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161042.4

ORF Insert Sequence:

ATGTCTCAGC AGGTCACACC CACCACAACA TCTCAGGTCT CCACAGTGAA ACCTGCACAA 
TATGAGAAAG GAGCTACACA ATCGTCTACA GCAGCTGCGA CTGTCAAATC CGGCGCTGGA 
GATCCTGCAA CCACCCAGAA AGGATCTTCT CAAGTCAAAC CTCAGGTGCA GGTTGAAGTG 
GGTCCTTCAG CAGCAAAAGT CAGTGCAGAT GCTGACGATC CGTTTGATGC CCTGTCTGGC 
ACATTACCAT CATCACAACC TTTGGCTCCT AAAGTCCACT ACACTGGACC TGAGATCAAA 
GAGCCCAAAC TGAAATCAGA GAAAGGTGTT TTGTGTGGTG AGCGAGATGA CACGCTGCCG 
CCCGGATACA GAAAGGAAGA CCTGGAAAAG AAGCATGGAG CTGGAGTTCC AGCTGGAGTT 
CCGGAGAAGC CTAAAGAAGT TCCCAAGCCC ATAAGCACAG ATGATGCACT GGAATCTCTC 
TCCTCTACGT TTGTGTCTCC AGCTCAAGCT GCTCCAAAGA AGACAGACGT GAAAACTGAA 
ACAATAGGAG CAGTGGATGT TAAATCGGCA GGCACCACTA ACTTTGCTCC CCCTCCACCA 
TCTCAGCAGA AACAACCGGC GTCGTCTCAA GCTGCTGCTG TAACCAAATC ACCTGCTCCA 
CCGGCAGACA AGAAAGCTAA AATGGAGACT CATGCACAAC CAGCCAAAGC AAAGACAGAG 
TCGGACAGCT CCATATCGCT GGACGCTCTC GATGCTTTGA GCGACACACT GGGGGCTTCT 
AAACCACCCC AAAAATTACC AGAACTCAAA CCTGGACAGA TAGTTGATGA GAAGAAACAG 
ACCTCAGAGA AAGGTGTTCG TGTTGGAGAA AGAGACGACA CACTTCCACC ACAATACAGA 
TTCAAAGAGG AAGATCTCAA GAAATATCCT CCACCTGCGA AAGAGCCTTC ACTGAACACA 
GATGATGCGA TGGACATACT TTCAGGAGAT TTCACGACCC CTGCATCGGC ACCTGTTGTC 
AAAGCTCCTG TTCCTCCTGC ACAGGACACA AAGAAGCATG CTGCAGTGCC TGAGAAGACC 
AAAGATATTC CCAAGACTAA AGCTGATGCT CTGTCAAGTG ATTTCGTGGC TCCGGCACAT 
TCTGCTCCTA AGGTTTCTTC AGCTGCTCCT AAAACCATCC CTCCAGGCCC ACCACAAACC 
CCAGATGAGG ATCCTTTCAG TGCTTTGGGC GACACTCTTT CTGCCCCAGA ACCACCAAAA 
AAACAACCTG ACCTGAAACC TAAGGACATC GTACATGAGA AGGATGTGAC GTCAAAGAAA 
GGAGTTCGTG TTGGAGAAAG AGACGACACA CTCCCACCAG AATACAGATT CAAAGAGGAA 
GATCTCAAGA AATATCCTCC TCCTCAGAAA GATCCTTCCT TAGATGCTAA TGAAGCTCTG 
GATATTCTGT CTGGGGATTT TACAACTCCT TCCGCACCAT CGGCTGTTTC CCCTGCCTCT 
AAATCCTCTG CCAAGTCTTC AGATTCAGCT TTAGATGCTC TTGCTGGTGA TTTCGTCGCT 
TCTTCTTCTG CGTCTTCAGT TCATTCTGCC GTCTCTGGCC CCCCACGCTC CGACAGACTG 
TTGTCAGACG GAACCTCATC AGCTCTAGAT GCCCTGTCAG AAACTCTGGG AGACATTAAA 
GCAGCCCCTG AACCCGCCCC TGTCCTACCT AAAACTGTCG TTAAGGAGAA GACGGTCATA 
GAAGAGAGAG TGAGTAAACC GGGTGAGAGA GACGACTCCC TTCCACCAGA TCAGCGGTTC 
ACTGCGGAGG ATCGCAAGGC ATTTGAAGAA GCAAAGAAGA AAGACGCCAA ACCAAAACAG 
CCATCAATGG ACGATTCAGC GGCTCTGGAC ATGTTGTCTA GTGATTTTTC AGATGTACCG 
ACTGTAAAGC CCACTGTATC TGATGGCAAA AGCTTCACCC CTGAACCAAC ACCACCAACC 
TTTAAGGCAA CCGGTCCGGT TTTAGACAAA CTGGCAGACA ACGTGCTTTC CAACCTGACT 
GACCCCAAAG CCAAAGACAG CAAAGCAAAG GGAAAGGGGG CGAAACCAAA GTCTAAACCA 
AAGAAACAGG CAGTGGAAGA CTCTTCAGCC ACAGATCTGC TGTCCAGTAA ACCGAGCTCA 
GATGTGGTGC CTTCAGCTTC CACAAAGGCT GGAAAAAGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_005161046.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161103.1 Danio rerio calpastatin (cast), transcript variant X27, mRNA.

Price & Availability↑

CloneID	ODa25622
Clone ID Related Accession (Same CDS sequence)	XM_005161046.4 , XM_005161046.3
Accession Version	XM_005161046.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calpastatin isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005161046.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGTCTCA GCAGGTCACA CCCACCACAA CATCTCAGGT CTCCACAGTG 
AAACCTGCAC AATATGAGGT GCAGGTTGAA GTGGGTCCTT CAGCAGCAAA AGTCAGTGCA 
GATGCTGACG ATCCGTTTGA TGCCCTGTCT GGCACATTAC CATCATCACA ACCTTTGGCT 
CCTAAAGTCC ACTACACTGG ACCTGAGATC AAAGAGCCCA AACTGAAATC AGAGAAAGGT 
GTTTTGTGTG GTGAGCGAGA TGACACGCTG CCGCCCGGAT ACAGAAAGGA AGACCTGGAA 
AAGAAGCATG GAGCTGGAGT TCCAGCTGGA GTTCCGGAGA AGCCTAAAGA AGTTCCCAAG 
CCCATAAGCA CAGATGATGC ACTGGAATCT CTCTCCTCTA CGTTTGTGTC TCCAGCTCAA 
GCTGCTCCAA AGAAGACAGA CGTGAAAACT GAAACAATAG GAGCAGTGGA TGTTAAATCG 
GCAGGCACCA CTAACTTTGC TCCCCCTCCA CCATCTCAGC AGAAACAACC GGCGTCGTCT 
CAAGCTGCTG CTGTAACCAA ATCACCTGCT CCACCGGCAG ACAAGAAAGC TAAAATGGAG 
ACTCATGCAC AACCAGCCAA AGCAAAGACA GAGTCGGACA GCTCCATATC GCTGGACGCT 
CTCGATGCTT TGAGCGACAC ACTGGGGGCT TCTAAACCAC CCCAAAAATT ACCAGAACTC 
AAACCTGGAC AGATAGTTGA TGAGAAGAAA CAGACCTCAG AGAAAGGTGT TCGTGTTGGA 
GAAAGAGACG ACACACTTCC ACCACAATAC AGATTCAAAG AGGAAGATCT CAAGAAATAT 
CCTCCACCTG CGAAAGAGCC TTCACTGAAC ACAGATGATG CGATGGACAT ACTTTCAGGA 
GATTTCACGA CCCCTGCATC GGCACCTGTT GTCAAAGCTC CTGTTCCTCC TGCACAGGAC 
ACAAAGAAGC ATGCTGCAGT GCCTGAGAAG ACCAAAGATA TTCCCAAGAC TAAAGCTGAT 
GCTCTGTCAA GTGATTTCGT GGCTCCGGCA CATTCTGCTC CTAAGGTTTC TTCAGCTGCT 
CCTAAAACCA TCCCTCCAGG CCCACCACAA ACCCCAGATG AGGATCCTTT CAGTGCTTTG 
GGCGACACTC TTTCTGCCCC AGAACCACCA AAAAAACAAC CTGACCTGAA ACCTAAGGAC 
ATCGTACATG AGAAGGATGT GACGTCAAAG AAAGGAGTTC GTGTTGGAGA AAGAGACGAC 
ACACTCCCAC CAGAATACAG ATTCAAAGAG GAAGATCTCA AGAAATATCC TCCTCCTCAG 
AAAGATCCTT CCTTAGATGC TAATGAAGCT CTGGATATTC TGTCTGGGGA TTTTACAACT 
CCTTCCGCAC CATCGGCTGT TTCCCCTGCC TCTAAATCCT CTGCCAAGTC TTCAGATTCA 
GCTTTAGATG CTCTTGCTGG TGATTTCGTC GCTTCTTCTT CTGCGTCTTC AGTTCATTCT 
GCCGTCTCTG GCCCCCCACG CTCCGACAGA CTGTTGTCAG ACGGAACCTC ATCAGCTCTA 
GATGCCCTGT CAGAAACTCT GGGAGACATT AAAGCAGCCC CTGAACCCGC CCCTGTCCTA 
CCTAAAACTG TCGTTAAGGA GAAGACGGTC ATAGAAGAGA GAGTGAGTAA ACCGGGTGAG 
AGAGACGACT CCCTTCCACC AGATCAGCGG TTCACTGCGG AGGATCGCAA GGCATTTGAA 
GAAGCAAAGA AGAAAGACGC CAAACCAAAA CAGCCATCAA TGGACGATTC AGCGGCTCTG 
GACATGTTGT CTAGTGATTT TTCAGATGTA CCGACTGTAA AGCCCACTGT ATCTGATGGC 
AAAAGCTTCA CCCCTGAACC AACACCACCA ACCTTTAAGG CAACCGGTCC GGTTTTAGAC 
AAACTGGCAG ACAACGTGCT TTCCAACCTG ACTGACCCCA AAGCCAAAGA CAGCAAAGCA 
AAGGGAAAGG GGGCGAAACC AAAGTCTAAA CCAAAGAAAC AGGCAGTGGA AGACTCTTCA 
GCCACAGATC TGCTGTCCAG TAAACCGAGC TCAGATGTGG TGCCTTCAGC TTCCACAAAG 
GCTGGAAAAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161103.1
CDS	72..2246
Translation	MGQIFSWIRGTRDQPALQDIAVEQQSQQVTPTTTSQVSTVKPAQYEVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_005161046.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161046.4

ORF Insert Sequence:

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGTCTCA GCAGGTCACA CCCACCACAA CATCTCAGGT CTCCACAGTG 
AAACCTGCAC AATATGAGGT GCAGGTTGAA GTGGGTCCTT CAGCAGCAAA AGTCAGTGCA 
GATGCTGACG ATCCGTTTGA TGCCCTGTCT GGCACATTAC CATCATCACA ACCTTTGGCT 
CCTAAAGTCC ACTACACTGG ACCTGAGATC AAAGAGCCCA AACTGAAATC AGAGAAAGGT 
GTTTTGTGTG GTGAGCGAGA TGACACGCTG CCGCCCGGAT ACAGAAAGGA AGACCTGGAA 
AAGAAGCATG GAGCTGGAGT TCCAGCTGGA GTTCCGGAGA AGCCTAAAGA AGTTCCCAAG 
CCCATAAGCA CAGATGATGC ACTGGAATCT CTCTCCTCTA CGTTTGTGTC TCCAGCTCAA 
GCTGCTCCAA AGAAGACAGA CGTGAAAACT GAAACAATAG GAGCAGTGGA TGTTAAATCG 
GCAGGCACCA CTAACTTTGC TCCCCCTCCA CCATCTCAGC AGAAACAACC GGCGTCGTCT 
CAAGCTGCTG CTGTAACCAA ATCACCTGCT CCACCGGCAG ACAAGAAAGC TAAAATGGAG 
ACTCATGCAC AACCAGCCAA AGCAAAGACA GAGTCGGACA GCTCCATATC GCTGGACGCT 
CTCGATGCTT TGAGCGACAC ACTGGGGGCT TCTAAACCAC CCCAAAAATT ACCAGAACTC 
AAACCTGGAC AGATAGTTGA TGAGAAGAAA CAGACCTCAG AGAAAGGTGT TCGTGTTGGA 
GAAAGAGACG ACACACTTCC ACCACAATAC AGATTCAAAG AGGAAGATCT CAAGAAATAT 
CCTCCACCTG CGAAAGAGCC TTCACTGAAC ACAGATGATG CGATGGACAT ACTTTCAGGA 
GATTTCACGA CCCCTGCATC GGCACCTGTT GTCAAAGCTC CTGTTCCTCC TGCACAGGAC 
ACAAAGAAGC ATGCTGCAGT GCCTGAGAAG ACCAAAGATA TTCCCAAGAC TAAAGCTGAT 
GCTCTGTCAA GTGATTTCGT GGCTCCGGCA CATTCTGCTC CTAAGGTTTC TTCAGCTGCT 
CCTAAAACCA TCCCTCCAGG CCCACCACAA ACCCCAGATG AGGATCCTTT CAGTGCTTTG 
GGCGACACTC TTTCTGCCCC AGAACCACCA AAAAAACAAC CTGACCTGAA ACCTAAGGAC 
ATCGTACATG AGAAGGATGT GACGTCAAAG AAAGGAGTTC GTGTTGGAGA AAGAGACGAC 
ACACTCCCAC CAGAATACAG ATTCAAAGAG GAAGATCTCA AGAAATATCC TCCTCCTCAG 
AAAGATCCTT CCTTAGATGC TAATGAAGCT CTGGATATTC TGTCTGGGGA TTTTACAACT 
CCTTCCGCAC CATCGGCTGT TTCCCCTGCC TCTAAATCCT CTGCCAAGTC TTCAGATTCA 
GCTTTAGATG CTCTTGCTGG TGATTTCGTC GCTTCTTCTT CTGCGTCTTC AGTTCATTCT 
GCCGTCTCTG GCCCCCCACG CTCCGACAGA CTGTTGTCAG ACGGAACCTC ATCAGCTCTA 
GATGCCCTGT CAGAAACTCT GGGAGACATT AAAGCAGCCC CTGAACCCGC CCCTGTCCTA 
CCTAAAACTG TCGTTAAGGA GAAGACGGTC ATAGAAGAGA GAGTGAGTAA ACCGGGTGAG 
AGAGACGACT CCCTTCCACC AGATCAGCGG TTCACTGCGG AGGATCGCAA GGCATTTGAA 
GAAGCAAAGA AGAAAGACGC CAAACCAAAA CAGCCATCAA TGGACGATTC AGCGGCTCTG 
GACATGTTGT CTAGTGATTT TTCAGATGTA CCGACTGTAA AGCCCACTGT ATCTGATGGC 
AAAAGCTTCA CCCCTGAACC AACACCACCA ACCTTTAAGG CAACCGGTCC GGTTTTAGAC 
AAACTGGCAG ACAACGTGCT TTCCAACCTG ACTGACCCCA AAGCCAAAGA CAGCAAAGCA 
AAGGGAAAGG GGGCGAAACC AAAGTCTAAA CCAAAGAAAC AGGCAGTGGA AGACTCTTCA 
GCCACAGATC TGCTGTCCAG TAAACCGAGC TCAGATGTGG TGCCTTCAGC TTCCACAAAG 
GCTGGAAAAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_005161046.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161103.1 Danio rerio calpastatin (cast), transcript variant X23, mRNA.

Price & Availability↑

CloneID	ODa25622
Clone ID Related Accession (Same CDS sequence)	XM_005161046.4 , XM_005161046.3
Accession Version	XM_005161046.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	calpastatin isoform X23
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005161046.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGTCTCA GCAGGTCACA CCCACCACAA CATCTCAGGT CTCCACAGTG 
AAACCTGCAC AATATGAGGT GCAGGTTGAA GTGGGTCCTT CAGCAGCAAA AGTCAGTGCA 
GATGCTGACG ATCCGTTTGA TGCCCTGTCT GGCACATTAC CATCATCACA ACCTTTGGCT 
CCTAAAGTCC ACTACACTGG ACCTGAGATC AAAGAGCCCA AACTGAAATC AGAGAAAGGT 
GTTTTGTGTG GTGAGCGAGA TGACACGCTG CCGCCCGGAT ACAGAAAGGA AGACCTGGAA 
AAGAAGCATG GAGCTGGAGT TCCAGCTGGA GTTCCGGAGA AGCCTAAAGA AGTTCCCAAG 
CCCATAAGCA CAGATGATGC ACTGGAATCT CTCTCCTCTA CGTTTGTGTC TCCAGCTCAA 
GCTGCTCCAA AGAAGACAGA CGTGAAAACT GAAACAATAG GAGCAGTGGA TGTTAAATCG 
GCAGGCACCA CTAACTTTGC TCCCCCTCCA CCATCTCAGC AGAAACAACC GGCGTCGTCT 
CAAGCTGCTG CTGTAACCAA ATCACCTGCT CCACCGGCAG ACAAGAAAGC TAAAATGGAG 
ACTCATGCAC AACCAGCCAA AGCAAAGACA GAGTCGGACA GCTCCATATC GCTGGACGCT 
CTCGATGCTT TGAGCGACAC ACTGGGGGCT TCTAAACCAC CCCAAAAATT ACCAGAACTC 
AAACCTGGAC AGATAGTTGA TGAGAAGAAA CAGACCTCAG AGAAAGGTGT TCGTGTTGGA 
GAAAGAGACG ACACACTTCC ACCACAATAC AGATTCAAAG AGGAAGATCT CAAGAAATAT 
CCTCCACCTG CGAAAGAGCC TTCACTGAAC ACAGATGATG CGATGGACAT ACTTTCAGGA 
GATTTCACGA CCCCTGCATC GGCACCTGTT GTCAAAGCTC CTGTTCCTCC TGCACAGGAC 
ACAAAGAAGC ATGCTGCAGT GCCTGAGAAG ACCAAAGATA TTCCCAAGAC TAAAGCTGAT 
GCTCTGTCAA GTGATTTCGT GGCTCCGGCA CATTCTGCTC CTAAGGTTTC TTCAGCTGCT 
CCTAAAACCA TCCCTCCAGG CCCACCACAA ACCCCAGATG AGGATCCTTT CAGTGCTTTG 
GGCGACACTC TTTCTGCCCC AGAACCACCA AAAAAACAAC CTGACCTGAA ACCTAAGGAC 
ATCGTACATG AGAAGGATGT GACGTCAAAG AAAGGAGTTC GTGTTGGAGA AAGAGACGAC 
ACACTCCCAC CAGAATACAG ATTCAAAGAG GAAGATCTCA AGAAATATCC TCCTCCTCAG 
AAAGATCCTT CCTTAGATGC TAATGAAGCT CTGGATATTC TGTCTGGGGA TTTTACAACT 
CCTTCCGCAC CATCGGCTGT TTCCCCTGCC TCTAAATCCT CTGCCAAGTC TTCAGATTCA 
GCTTTAGATG CTCTTGCTGG TGATTTCGTC GCTTCTTCTT CTGCGTCTTC AGTTCATTCT 
GCCGTCTCTG GCCCCCCACG CTCCGACAGA CTGTTGTCAG ACGGAACCTC ATCAGCTCTA 
GATGCCCTGT CAGAAACTCT GGGAGACATT AAAGCAGCCC CTGAACCCGC CCCTGTCCTA 
CCTAAAACTG TCGTTAAGGA GAAGACGGTC ATAGAAGAGA GAGTGAGTAA ACCGGGTGAG 
AGAGACGACT CCCTTCCACC AGATCAGCGG TTCACTGCGG AGGATCGCAA GGCATTTGAA 
GAAGCAAAGA AGAAAGACGC CAAACCAAAA CAGCCATCAA TGGACGATTC AGCGGCTCTG 
GACATGTTGT CTAGTGATTT TTCAGATGTA CCGACTGTAA AGCCCACTGT ATCTGATGGC 
AAAAGCTTCA CCCCTGAACC AACACCACCA ACCTTTAAGG CAACCGGTCC GGTTTTAGAC 
AAACTGGCAG ACAACGTGCT TTCCAACCTG ACTGACCCCA AAGCCAAAGA CAGCAAAGCA 
AAGGGAAAGG GGGCGAAACC AAAGTCTAAA CCAAAGAAAC AGGCAGTGGA AGACTCTTCA 
GCCACAGATC TGCTGTCCAG TAAACCGAGC TCAGATGTGG TGCCTTCAGC TTCCACAAAG 
GCTGGAAAAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161103.1
CDS	67..2241
Translation	MGQIFSWIRGTRDQPALQDIAVEQQSQQVTPTTTSQVSTVKPAQYEVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_005161046.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161046.3

ORF Insert Sequence:

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGTCTCA GCAGGTCACA CCCACCACAA CATCTCAGGT CTCCACAGTG 
AAACCTGCAC AATATGAGGT GCAGGTTGAA GTGGGTCCTT CAGCAGCAAA AGTCAGTGCA 
GATGCTGACG ATCCGTTTGA TGCCCTGTCT GGCACATTAC CATCATCACA ACCTTTGGCT 
CCTAAAGTCC ACTACACTGG ACCTGAGATC AAAGAGCCCA AACTGAAATC AGAGAAAGGT 
GTTTTGTGTG GTGAGCGAGA TGACACGCTG CCGCCCGGAT ACAGAAAGGA AGACCTGGAA 
AAGAAGCATG GAGCTGGAGT TCCAGCTGGA GTTCCGGAGA AGCCTAAAGA AGTTCCCAAG 
CCCATAAGCA CAGATGATGC ACTGGAATCT CTCTCCTCTA CGTTTGTGTC TCCAGCTCAA 
GCTGCTCCAA AGAAGACAGA CGTGAAAACT GAAACAATAG GAGCAGTGGA TGTTAAATCG 
GCAGGCACCA CTAACTTTGC TCCCCCTCCA CCATCTCAGC AGAAACAACC GGCGTCGTCT 
CAAGCTGCTG CTGTAACCAA ATCACCTGCT CCACCGGCAG ACAAGAAAGC TAAAATGGAG 
ACTCATGCAC AACCAGCCAA AGCAAAGACA GAGTCGGACA GCTCCATATC GCTGGACGCT 
CTCGATGCTT TGAGCGACAC ACTGGGGGCT TCTAAACCAC CCCAAAAATT ACCAGAACTC 
AAACCTGGAC AGATAGTTGA TGAGAAGAAA CAGACCTCAG AGAAAGGTGT TCGTGTTGGA 
GAAAGAGACG ACACACTTCC ACCACAATAC AGATTCAAAG AGGAAGATCT CAAGAAATAT 
CCTCCACCTG CGAAAGAGCC TTCACTGAAC ACAGATGATG CGATGGACAT ACTTTCAGGA 
GATTTCACGA CCCCTGCATC GGCACCTGTT GTCAAAGCTC CTGTTCCTCC TGCACAGGAC 
ACAAAGAAGC ATGCTGCAGT GCCTGAGAAG ACCAAAGATA TTCCCAAGAC TAAAGCTGAT 
GCTCTGTCAA GTGATTTCGT GGCTCCGGCA CATTCTGCTC CTAAGGTTTC TTCAGCTGCT 
CCTAAAACCA TCCCTCCAGG CCCACCACAA ACCCCAGATG AGGATCCTTT CAGTGCTTTG 
GGCGACACTC TTTCTGCCCC AGAACCACCA AAAAAACAAC CTGACCTGAA ACCTAAGGAC 
ATCGTACATG AGAAGGATGT GACGTCAAAG AAAGGAGTTC GTGTTGGAGA AAGAGACGAC 
ACACTCCCAC CAGAATACAG ATTCAAAGAG GAAGATCTCA AGAAATATCC TCCTCCTCAG 
AAAGATCCTT CCTTAGATGC TAATGAAGCT CTGGATATTC TGTCTGGGGA TTTTACAACT 
CCTTCCGCAC CATCGGCTGT TTCCCCTGCC TCTAAATCCT CTGCCAAGTC TTCAGATTCA 
GCTTTAGATG CTCTTGCTGG TGATTTCGTC GCTTCTTCTT CTGCGTCTTC AGTTCATTCT 
GCCGTCTCTG GCCCCCCACG CTCCGACAGA CTGTTGTCAG ACGGAACCTC ATCAGCTCTA 
GATGCCCTGT CAGAAACTCT GGGAGACATT AAAGCAGCCC CTGAACCCGC CCCTGTCCTA 
CCTAAAACTG TCGTTAAGGA GAAGACGGTC ATAGAAGAGA GAGTGAGTAA ACCGGGTGAG 
AGAGACGACT CCCTTCCACC AGATCAGCGG TTCACTGCGG AGGATCGCAA GGCATTTGAA 
GAAGCAAAGA AGAAAGACGC CAAACCAAAA CAGCCATCAA TGGACGATTC AGCGGCTCTG 
GACATGTTGT CTAGTGATTT TTCAGATGTA CCGACTGTAA AGCCCACTGT ATCTGATGGC 
AAAAGCTTCA CCCCTGAACC AACACCACCA ACCTTTAAGG CAACCGGTCC GGTTTTAGAC 
AAACTGGCAG ACAACGTGCT TTCCAACCTG ACTGACCCCA AAGCCAAAGA CAGCAAAGCA 
AAGGGAAAGG GGGCGAAACC AAAGTCTAAA CCAAAGAAAC AGGCAGTGGA AGACTCTTCA 
GCCACAGATC TGCTGTCCAG TAAACCGAGC TCAGATGTGG TGCCTTCAGC TTCCACAAAG 
GCTGGAAAAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_005161048.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161105.1 Danio rerio calpastatin (cast), transcript variant X30, mRNA.

Price & Availability↑

CloneID	ODa25624
Clone ID Related Accession (Same CDS sequence)	XM_005161048.4 , XM_005161049.3 , XM_005161048.3 , XM_005161049.4
Accession Version	XM_005161048.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calpastatin isoform X30
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005161048.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC AGGTCACACC CACCACAACA TCTCAGGTCT CCACAGTGAA ACCTGCACAA 
TATGAGGTGC AGGTTGAAGT GGGTCCTTCA GCAGCAAAAG TCAGTGCAGA TGCTGACGAT 
CCGTTTGATG CCCTGTCTGG CACATTACCA TCATCACAAC CTTTGGCTCC TAAAGTCCAC 
TACACTGGAC CTGAGATCAA AGAGCCCAAA CTGAAATCAG AGAAAGGTGT TTTGTGTGGT 
GAGCGAGATG ACACGCTGCC GCCCGGATAC AGAAAGGAAG ACCTGGAAAA GAAGCATGGA 
GCTGGAGTTC CAGCTGGAGT TCCGGAGAAG CCTAAAGAAG TTCCCAAGCC CATAAGCACA 
GATGATGCAC TGGAATCTCT CTCCTCTACG TTTGTGTCTC CAGCTCAAGC TGCTCCAAAG 
AAGACAGACG TGAAAACTGA AACAATAGGA GCAGTGGATG TTAAATCGGC AGGCACCACT 
AACTTTGCTC CCCCTCCACC ATCTCAGCAG AAACAACCGG CGTCGTCTCA AGCTGCTGCT 
GTAACCAAAT CACCTGCTCC ACCGGCAGAC AAGAAAGCTA AAATGGAGAC TCATGCACAA 
CCAGCCAAAG CAAAGACAGA GTCGGACAGC TCCATATCGC TGGACGCTCT CGATGCTTTG 
AGCGACACAC TGGGGGCTTC TAAACCACCC CAAAAATTAC CAGAACTCAA ACCTGGACAG 
ATAGTTGATG AGAAGAAACA GACCTCAGAG AAAGGTGTTC GTGTTGGAGA AAGAGACGAC 
ACACTTCCAC CACAATACAG ATTCAAAGAG GAAGATCTCA AGAAATATCC TCCACCTGCG 
AAAGAGCCTT CACTGAACAC AGATGATGCG ATGGACATAC TTTCAGGAGA TTTCACGACC 
CCTGCATCGG CACCTGTTGT CAAAGCTCCT GTTCCTCCTG CACAGGACAC AAAGAAGCAT 
GCTGCAGTGC CTGAGAAGAC CAAAGATATT CCCAAGACTA AAGCTGATGC TCTGTCAAGT 
GATTTCGTGG CTCCGGCACA TTCTGCTCCT AAGGTTTCTT CAGCTGCTCC TAAAACCATC 
CCTCCAGGCC CACCACAAAC CCCAGATGAG GATCCTTTCA GTGCTTTGGG CGACACTCTT 
TCTGCCCCAG AACCACCAAA AAAACAACCT GACCTGAAAC CTAAGGACAT CGTACATGAG 
AAGGATGTGA CGTCAAAGAA AGGAGTTCGT GTTGGAGAAA GAGACGACAC ACTCCCACCA 
GAATACAGAT TCAAAGAGGA AGATCTCAAG AAATATCCTC CTCCTCAGAA AGATCCTTCC 
TTAGATGCTA ATGAAGCTCT GGATATTCTG TCTGGGGATT TTACAACTCC TTCCGCACCA 
TCGGCTGTTT CCCCTGCCTC TAAATCCTCT GCCAAGTCTT CAGATTCAGC TTTAGATGCT 
CTTGCTGGTG ATTTCGTCGC TTCTTCTTCT GCGTCTTCAG TTCATTCTGC CGTCTCTGGC 
CCCCCACGCT CCGACAGACT GTTGTCAGAC GGAACCTCAT CAGCTCTAGA TGCCCTGTCA 
GAAACTCTGG GAGACATTAA AGCAGCCCCT GAACCCGCCC CTGTCCTACC TAAAACTGTC 
GTTAAGGAGA AGACGGTCAT AGAAGAGAGA GTGAGTAAAC CGGGTGAGAG AGACGACTCC 
CTTCCACCAG ATCAGCGGTT CACTGCGGAG GATCGCAAGG CATTTGAAGA AGCAAAGAAG 
AAAGACGCCA AACCAAAACA GCCATCAATG GACGATTCAG CGGCTCTGGA CATGTTGTCT 
AGTGATTTTT CAGATGTACC GACTGTAAAG CCCACTGTAT CTGATGGCAA AAGCTTCACC 
CCTGAACCAA CACCACCAAC CTTTAAGGCA ACCGGTCCGG TTTTAGACAA ACTGGCAGAC 
AACGTGCTTT CCAACCTGAC TGACCCCAAA GCCAAAGACA GCAAAGCAAA GGGAAAGGGG 
GCGAAACCAA AGTCTAAACC AAAGAAACAG GCAGTGGAAG ACTCTTCAGC CACAGATCTG 
CTGTCCAGTA AACCGAGCTC AGATGTGGTG CCTTCAGCTT CCACAAAGGC TGGAAAAAGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161105.1
CDS	148..2250
Translation	MSQQVTPTTTSQVSTVKPAQYEVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_005161048.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161048.4

ORF Insert Sequence:

ATGTCTCAGC AGGTCACACC CACCACAACA TCTCAGGTCT CCACAGTGAA ACCTGCACAA 
TATGAGGTGC AGGTTGAAGT GGGTCCTTCA GCAGCAAAAG TCAGTGCAGA TGCTGACGAT 
CCGTTTGATG CCCTGTCTGG CACATTACCA TCATCACAAC CTTTGGCTCC TAAAGTCCAC 
TACACTGGAC CTGAGATCAA AGAGCCCAAA CTGAAATCAG AGAAAGGTGT TTTGTGTGGT 
GAGCGAGATG ACACGCTGCC GCCCGGATAC AGAAAGGAAG ACCTGGAAAA GAAGCATGGA 
GCTGGAGTTC CAGCTGGAGT TCCGGAGAAG CCTAAAGAAG TTCCCAAGCC CATAAGCACA 
GATGATGCAC TGGAATCTCT CTCCTCTACG TTTGTGTCTC CAGCTCAAGC TGCTCCAAAG 
AAGACAGACG TGAAAACTGA AACAATAGGA GCAGTGGATG TTAAATCGGC AGGCACCACT 
AACTTTGCTC CCCCTCCACC ATCTCAGCAG AAACAACCGG CGTCGTCTCA AGCTGCTGCT 
GTAACCAAAT CACCTGCTCC ACCGGCAGAC AAGAAAGCTA AAATGGAGAC TCATGCACAA 
CCAGCCAAAG CAAAGACAGA GTCGGACAGC TCCATATCGC TGGACGCTCT CGATGCTTTG 
AGCGACACAC TGGGGGCTTC TAAACCACCC CAAAAATTAC CAGAACTCAA ACCTGGACAG 
ATAGTTGATG AGAAGAAACA GACCTCAGAG AAAGGTGTTC GTGTTGGAGA AAGAGACGAC 
ACACTTCCAC CACAATACAG ATTCAAAGAG GAAGATCTCA AGAAATATCC TCCACCTGCG 
AAAGAGCCTT CACTGAACAC AGATGATGCG ATGGACATAC TTTCAGGAGA TTTCACGACC 
CCTGCATCGG CACCTGTTGT CAAAGCTCCT GTTCCTCCTG CACAGGACAC AAAGAAGCAT 
GCTGCAGTGC CTGAGAAGAC CAAAGATATT CCCAAGACTA AAGCTGATGC TCTGTCAAGT 
GATTTCGTGG CTCCGGCACA TTCTGCTCCT AAGGTTTCTT CAGCTGCTCC TAAAACCATC 
CCTCCAGGCC CACCACAAAC CCCAGATGAG GATCCTTTCA GTGCTTTGGG CGACACTCTT 
TCTGCCCCAG AACCACCAAA AAAACAACCT GACCTGAAAC CTAAGGACAT CGTACATGAG 
AAGGATGTGA CGTCAAAGAA AGGAGTTCGT GTTGGAGAAA GAGACGACAC ACTCCCACCA 
GAATACAGAT TCAAAGAGGA AGATCTCAAG AAATATCCTC CTCCTCAGAA AGATCCTTCC 
TTAGATGCTA ATGAAGCTCT GGATATTCTG TCTGGGGATT TTACAACTCC TTCCGCACCA 
TCGGCTGTTT CCCCTGCCTC TAAATCCTCT GCCAAGTCTT CAGATTCAGC TTTAGATGCT 
CTTGCTGGTG ATTTCGTCGC TTCTTCTTCT GCGTCTTCAG TTCATTCTGC CGTCTCTGGC 
CCCCCACGCT CCGACAGACT GTTGTCAGAC GGAACCTCAT CAGCTCTAGA TGCCCTGTCA 
GAAACTCTGG GAGACATTAA AGCAGCCCCT GAACCCGCCC CTGTCCTACC TAAAACTGTC 
GTTAAGGAGA AGACGGTCAT AGAAGAGAGA GTGAGTAAAC CGGGTGAGAG AGACGACTCC 
CTTCCACCAG ATCAGCGGTT CACTGCGGAG GATCGCAAGG CATTTGAAGA AGCAAAGAAG 
AAAGACGCCA AACCAAAACA GCCATCAATG GACGATTCAG CGGCTCTGGA CATGTTGTCT 
AGTGATTTTT CAGATGTACC GACTGTAAAG CCCACTGTAT CTGATGGCAA AAGCTTCACC 
CCTGAACCAA CACCACCAAC CTTTAAGGCA ACCGGTCCGG TTTTAGACAA ACTGGCAGAC 
AACGTGCTTT CCAACCTGAC TGACCCCAAA GCCAAAGACA GCAAAGCAAA GGGAAAGGGG 
GCGAAACCAA AGTCTAAACC AAAGAAACAG GCAGTGGAAG ACTCTTCAGC CACAGATCTG 
CTGTCCAGTA AACCGAGCTC AGATGTGGTG CCTTCAGCTT CCACAAAGGC TGGAAAAAGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_005161049.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161106.1 Danio rerio calpastatin (cast), transcript variant X25, mRNA.

Price & Availability↑

CloneID	ODa25624
Clone ID Related Accession (Same CDS sequence)	XM_005161048.4 , XM_005161049.3 , XM_005161048.3 , XM_005161049.4
Accession Version	XM_005161049.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	calpastatin isoform X25
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005161049.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC AGGTCACACC CACCACAACA TCTCAGGTCT CCACAGTGAA ACCTGCACAA 
TATGAGGTGC AGGTTGAAGT GGGTCCTTCA GCAGCAAAAG TCAGTGCAGA TGCTGACGAT 
CCGTTTGATG CCCTGTCTGG CACATTACCA TCATCACAAC CTTTGGCTCC TAAAGTCCAC 
TACACTGGAC CTGAGATCAA AGAGCCCAAA CTGAAATCAG AGAAAGGTGT TTTGTGTGGT 
GAGCGAGATG ACACGCTGCC GCCCGGATAC AGAAAGGAAG ACCTGGAAAA GAAGCATGGA 
GCTGGAGTTC CAGCTGGAGT TCCGGAGAAG CCTAAAGAAG TTCCCAAGCC CATAAGCACA 
GATGATGCAC TGGAATCTCT CTCCTCTACG TTTGTGTCTC CAGCTCAAGC TGCTCCAAAG 
AAGACAGACG TGAAAACTGA AACAATAGGA GCAGTGGATG TTAAATCGGC AGGCACCACT 
AACTTTGCTC CCCCTCCACC ATCTCAGCAG AAACAACCGG CGTCGTCTCA AGCTGCTGCT 
GTAACCAAAT CACCTGCTCC ACCGGCAGAC AAGAAAGCTA AAATGGAGAC TCATGCACAA 
CCAGCCAAAG CAAAGACAGA GTCGGACAGC TCCATATCGC TGGACGCTCT CGATGCTTTG 
AGCGACACAC TGGGGGCTTC TAAACCACCC CAAAAATTAC CAGAACTCAA ACCTGGACAG 
ATAGTTGATG AGAAGAAACA GACCTCAGAG AAAGGTGTTC GTGTTGGAGA AAGAGACGAC 
ACACTTCCAC CACAATACAG ATTCAAAGAG GAAGATCTCA AGAAATATCC TCCACCTGCG 
AAAGAGCCTT CACTGAACAC AGATGATGCG ATGGACATAC TTTCAGGAGA TTTCACGACC 
CCTGCATCGG CACCTGTTGT CAAAGCTCCT GTTCCTCCTG CACAGGACAC AAAGAAGCAT 
GCTGCAGTGC CTGAGAAGAC CAAAGATATT CCCAAGACTA AAGCTGATGC TCTGTCAAGT 
GATTTCGTGG CTCCGGCACA TTCTGCTCCT AAGGTTTCTT CAGCTGCTCC TAAAACCATC 
CCTCCAGGCC CACCACAAAC CCCAGATGAG GATCCTTTCA GTGCTTTGGG CGACACTCTT 
TCTGCCCCAG AACCACCAAA AAAACAACCT GACCTGAAAC CTAAGGACAT CGTACATGAG 
AAGGATGTGA CGTCAAAGAA AGGAGTTCGT GTTGGAGAAA GAGACGACAC ACTCCCACCA 
GAATACAGAT TCAAAGAGGA AGATCTCAAG AAATATCCTC CTCCTCAGAA AGATCCTTCC 
TTAGATGCTA ATGAAGCTCT GGATATTCTG TCTGGGGATT TTACAACTCC TTCCGCACCA 
TCGGCTGTTT CCCCTGCCTC TAAATCCTCT GCCAAGTCTT CAGATTCAGC TTTAGATGCT 
CTTGCTGGTG ATTTCGTCGC TTCTTCTTCT GCGTCTTCAG TTCATTCTGC CGTCTCTGGC 
CCCCCACGCT CCGACAGACT GTTGTCAGAC GGAACCTCAT CAGCTCTAGA TGCCCTGTCA 
GAAACTCTGG GAGACATTAA AGCAGCCCCT GAACCCGCCC CTGTCCTACC TAAAACTGTC 
GTTAAGGAGA AGACGGTCAT AGAAGAGAGA GTGAGTAAAC CGGGTGAGAG AGACGACTCC 
CTTCCACCAG ATCAGCGGTT CACTGCGGAG GATCGCAAGG CATTTGAAGA AGCAAAGAAG 
AAAGACGCCA AACCAAAACA GCCATCAATG GACGATTCAG CGGCTCTGGA CATGTTGTCT 
AGTGATTTTT CAGATGTACC GACTGTAAAG CCCACTGTAT CTGATGGCAA AAGCTTCACC 
CCTGAACCAA CACCACCAAC CTTTAAGGCA ACCGGTCCGG TTTTAGACAA ACTGGCAGAC 
AACGTGCTTT CCAACCTGAC TGACCCCAAA GCCAAAGACA GCAAAGCAAA GGGAAAGGGG 
GCGAAACCAA AGTCTAAACC AAAGAAACAG GCAGTGGAAG ACTCTTCAGC CACAGATCTG 
CTGTCCAGTA AACCGAGCTC AGATGTGGTG CCTTCAGCTT CCACAAAGGC TGGAAAAAGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161106.1
CDS	229..2331
Translation	MSQQVTPTTTSQVSTVKPAQYEVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_005161049.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161049.3

ORF Insert Sequence:

ATGTCTCAGC AGGTCACACC CACCACAACA TCTCAGGTCT CCACAGTGAA ACCTGCACAA 
TATGAGGTGC AGGTTGAAGT GGGTCCTTCA GCAGCAAAAG TCAGTGCAGA TGCTGACGAT 
CCGTTTGATG CCCTGTCTGG CACATTACCA TCATCACAAC CTTTGGCTCC TAAAGTCCAC 
TACACTGGAC CTGAGATCAA AGAGCCCAAA CTGAAATCAG AGAAAGGTGT TTTGTGTGGT 
GAGCGAGATG ACACGCTGCC GCCCGGATAC AGAAAGGAAG ACCTGGAAAA GAAGCATGGA 
GCTGGAGTTC CAGCTGGAGT TCCGGAGAAG CCTAAAGAAG TTCCCAAGCC CATAAGCACA 
GATGATGCAC TGGAATCTCT CTCCTCTACG TTTGTGTCTC CAGCTCAAGC TGCTCCAAAG 
AAGACAGACG TGAAAACTGA AACAATAGGA GCAGTGGATG TTAAATCGGC AGGCACCACT 
AACTTTGCTC CCCCTCCACC ATCTCAGCAG AAACAACCGG CGTCGTCTCA AGCTGCTGCT 
GTAACCAAAT CACCTGCTCC ACCGGCAGAC AAGAAAGCTA AAATGGAGAC TCATGCACAA 
CCAGCCAAAG CAAAGACAGA GTCGGACAGC TCCATATCGC TGGACGCTCT CGATGCTTTG 
AGCGACACAC TGGGGGCTTC TAAACCACCC CAAAAATTAC CAGAACTCAA ACCTGGACAG 
ATAGTTGATG AGAAGAAACA GACCTCAGAG AAAGGTGTTC GTGTTGGAGA AAGAGACGAC 
ACACTTCCAC CACAATACAG ATTCAAAGAG GAAGATCTCA AGAAATATCC TCCACCTGCG 
AAAGAGCCTT CACTGAACAC AGATGATGCG ATGGACATAC TTTCAGGAGA TTTCACGACC 
CCTGCATCGG CACCTGTTGT CAAAGCTCCT GTTCCTCCTG CACAGGACAC AAAGAAGCAT 
GCTGCAGTGC CTGAGAAGAC CAAAGATATT CCCAAGACTA AAGCTGATGC TCTGTCAAGT 
GATTTCGTGG CTCCGGCACA TTCTGCTCCT AAGGTTTCTT CAGCTGCTCC TAAAACCATC 
CCTCCAGGCC CACCACAAAC CCCAGATGAG GATCCTTTCA GTGCTTTGGG CGACACTCTT 
TCTGCCCCAG AACCACCAAA AAAACAACCT GACCTGAAAC CTAAGGACAT CGTACATGAG 
AAGGATGTGA CGTCAAAGAA AGGAGTTCGT GTTGGAGAAA GAGACGACAC ACTCCCACCA 
GAATACAGAT TCAAAGAGGA AGATCTCAAG AAATATCCTC CTCCTCAGAA AGATCCTTCC 
TTAGATGCTA ATGAAGCTCT GGATATTCTG TCTGGGGATT TTACAACTCC TTCCGCACCA 
TCGGCTGTTT CCCCTGCCTC TAAATCCTCT GCCAAGTCTT CAGATTCAGC TTTAGATGCT 
CTTGCTGGTG ATTTCGTCGC TTCTTCTTCT GCGTCTTCAG TTCATTCTGC CGTCTCTGGC 
CCCCCACGCT CCGACAGACT GTTGTCAGAC GGAACCTCAT CAGCTCTAGA TGCCCTGTCA 
GAAACTCTGG GAGACATTAA AGCAGCCCCT GAACCCGCCC CTGTCCTACC TAAAACTGTC 
GTTAAGGAGA AGACGGTCAT AGAAGAGAGA GTGAGTAAAC CGGGTGAGAG AGACGACTCC 
CTTCCACCAG ATCAGCGGTT CACTGCGGAG GATCGCAAGG CATTTGAAGA AGCAAAGAAG 
AAAGACGCCA AACCAAAACA GCCATCAATG GACGATTCAG CGGCTCTGGA CATGTTGTCT 
AGTGATTTTT CAGATGTACC GACTGTAAAG CCCACTGTAT CTGATGGCAA AAGCTTCACC 
CCTGAACCAA CACCACCAAC CTTTAAGGCA ACCGGTCCGG TTTTAGACAA ACTGGCAGAC 
AACGTGCTTT CCAACCTGAC TGACCCCAAA GCCAAAGACA GCAAAGCAAA GGGAAAGGGG 
GCGAAACCAA AGTCTAAACC AAAGAAACAG GCAGTGGAAG ACTCTTCAGC CACAGATCTG 
CTGTCCAGTA AACCGAGCTC AGATGTGGTG CCTTCAGCTT CCACAAAGGC TGGAAAAAGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_005161048.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161105.1 Danio rerio calpastatin (cast), transcript variant X24, mRNA.

Price & Availability↑

CloneID	ODa25624
Clone ID Related Accession (Same CDS sequence)	XM_005161048.4 , XM_005161049.3 , XM_005161048.3 , XM_005161049.4
Accession Version	XM_005161048.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	calpastatin isoform X24
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005161048.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC AGGTCACACC CACCACAACA TCTCAGGTCT CCACAGTGAA ACCTGCACAA 
TATGAGGTGC AGGTTGAAGT GGGTCCTTCA GCAGCAAAAG TCAGTGCAGA TGCTGACGAT 
CCGTTTGATG CCCTGTCTGG CACATTACCA TCATCACAAC CTTTGGCTCC TAAAGTCCAC 
TACACTGGAC CTGAGATCAA AGAGCCCAAA CTGAAATCAG AGAAAGGTGT TTTGTGTGGT 
GAGCGAGATG ACACGCTGCC GCCCGGATAC AGAAAGGAAG ACCTGGAAAA GAAGCATGGA 
GCTGGAGTTC CAGCTGGAGT TCCGGAGAAG CCTAAAGAAG TTCCCAAGCC CATAAGCACA 
GATGATGCAC TGGAATCTCT CTCCTCTACG TTTGTGTCTC CAGCTCAAGC TGCTCCAAAG 
AAGACAGACG TGAAAACTGA AACAATAGGA GCAGTGGATG TTAAATCGGC AGGCACCACT 
AACTTTGCTC CCCCTCCACC ATCTCAGCAG AAACAACCGG CGTCGTCTCA AGCTGCTGCT 
GTAACCAAAT CACCTGCTCC ACCGGCAGAC AAGAAAGCTA AAATGGAGAC TCATGCACAA 
CCAGCCAAAG CAAAGACAGA GTCGGACAGC TCCATATCGC TGGACGCTCT CGATGCTTTG 
AGCGACACAC TGGGGGCTTC TAAACCACCC CAAAAATTAC CAGAACTCAA ACCTGGACAG 
ATAGTTGATG AGAAGAAACA GACCTCAGAG AAAGGTGTTC GTGTTGGAGA AAGAGACGAC 
ACACTTCCAC CACAATACAG ATTCAAAGAG GAAGATCTCA AGAAATATCC TCCACCTGCG 
AAAGAGCCTT CACTGAACAC AGATGATGCG ATGGACATAC TTTCAGGAGA TTTCACGACC 
CCTGCATCGG CACCTGTTGT CAAAGCTCCT GTTCCTCCTG CACAGGACAC AAAGAAGCAT 
GCTGCAGTGC CTGAGAAGAC CAAAGATATT CCCAAGACTA AAGCTGATGC TCTGTCAAGT 
GATTTCGTGG CTCCGGCACA TTCTGCTCCT AAGGTTTCTT CAGCTGCTCC TAAAACCATC 
CCTCCAGGCC CACCACAAAC CCCAGATGAG GATCCTTTCA GTGCTTTGGG CGACACTCTT 
TCTGCCCCAG AACCACCAAA AAAACAACCT GACCTGAAAC CTAAGGACAT CGTACATGAG 
AAGGATGTGA CGTCAAAGAA AGGAGTTCGT GTTGGAGAAA GAGACGACAC ACTCCCACCA 
GAATACAGAT TCAAAGAGGA AGATCTCAAG AAATATCCTC CTCCTCAGAA AGATCCTTCC 
TTAGATGCTA ATGAAGCTCT GGATATTCTG TCTGGGGATT TTACAACTCC TTCCGCACCA 
TCGGCTGTTT CCCCTGCCTC TAAATCCTCT GCCAAGTCTT CAGATTCAGC TTTAGATGCT 
CTTGCTGGTG ATTTCGTCGC TTCTTCTTCT GCGTCTTCAG TTCATTCTGC CGTCTCTGGC 
CCCCCACGCT CCGACAGACT GTTGTCAGAC GGAACCTCAT CAGCTCTAGA TGCCCTGTCA 
GAAACTCTGG GAGACATTAA AGCAGCCCCT GAACCCGCCC CTGTCCTACC TAAAACTGTC 
GTTAAGGAGA AGACGGTCAT AGAAGAGAGA GTGAGTAAAC CGGGTGAGAG AGACGACTCC 
CTTCCACCAG ATCAGCGGTT CACTGCGGAG GATCGCAAGG CATTTGAAGA AGCAAAGAAG 
AAAGACGCCA AACCAAAACA GCCATCAATG GACGATTCAG CGGCTCTGGA CATGTTGTCT 
AGTGATTTTT CAGATGTACC GACTGTAAAG CCCACTGTAT CTGATGGCAA AAGCTTCACC 
CCTGAACCAA CACCACCAAC CTTTAAGGCA ACCGGTCCGG TTTTAGACAA ACTGGCAGAC 
AACGTGCTTT CCAACCTGAC TGACCCCAAA GCCAAAGACA GCAAAGCAAA GGGAAAGGGG 
GCGAAACCAA AGTCTAAACC AAAGAAACAG GCAGTGGAAG ACTCTTCAGC CACAGATCTG 
CTGTCCAGTA AACCGAGCTC AGATGTGGTG CCTTCAGCTT CCACAAAGGC TGGAAAAAGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161105.1
CDS	128..2230
Translation	MSQQVTPTTTSQVSTVKPAQYEVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_005161048.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161048.3

ORF Insert Sequence:

ATGTCTCAGC AGGTCACACC CACCACAACA TCTCAGGTCT CCACAGTGAA ACCTGCACAA 
TATGAGGTGC AGGTTGAAGT GGGTCCTTCA GCAGCAAAAG TCAGTGCAGA TGCTGACGAT 
CCGTTTGATG CCCTGTCTGG CACATTACCA TCATCACAAC CTTTGGCTCC TAAAGTCCAC 
TACACTGGAC CTGAGATCAA AGAGCCCAAA CTGAAATCAG AGAAAGGTGT TTTGTGTGGT 
GAGCGAGATG ACACGCTGCC GCCCGGATAC AGAAAGGAAG ACCTGGAAAA GAAGCATGGA 
GCTGGAGTTC CAGCTGGAGT TCCGGAGAAG CCTAAAGAAG TTCCCAAGCC CATAAGCACA 
GATGATGCAC TGGAATCTCT CTCCTCTACG TTTGTGTCTC CAGCTCAAGC TGCTCCAAAG 
AAGACAGACG TGAAAACTGA AACAATAGGA GCAGTGGATG TTAAATCGGC AGGCACCACT 
AACTTTGCTC CCCCTCCACC ATCTCAGCAG AAACAACCGG CGTCGTCTCA AGCTGCTGCT 
GTAACCAAAT CACCTGCTCC ACCGGCAGAC AAGAAAGCTA AAATGGAGAC TCATGCACAA 
CCAGCCAAAG CAAAGACAGA GTCGGACAGC TCCATATCGC TGGACGCTCT CGATGCTTTG 
AGCGACACAC TGGGGGCTTC TAAACCACCC CAAAAATTAC CAGAACTCAA ACCTGGACAG 
ATAGTTGATG AGAAGAAACA GACCTCAGAG AAAGGTGTTC GTGTTGGAGA AAGAGACGAC 
ACACTTCCAC CACAATACAG ATTCAAAGAG GAAGATCTCA AGAAATATCC TCCACCTGCG 
AAAGAGCCTT CACTGAACAC AGATGATGCG ATGGACATAC TTTCAGGAGA TTTCACGACC 
CCTGCATCGG CACCTGTTGT CAAAGCTCCT GTTCCTCCTG CACAGGACAC AAAGAAGCAT 
GCTGCAGTGC CTGAGAAGAC CAAAGATATT CCCAAGACTA AAGCTGATGC TCTGTCAAGT 
GATTTCGTGG CTCCGGCACA TTCTGCTCCT AAGGTTTCTT CAGCTGCTCC TAAAACCATC 
CCTCCAGGCC CACCACAAAC CCCAGATGAG GATCCTTTCA GTGCTTTGGG CGACACTCTT 
TCTGCCCCAG AACCACCAAA AAAACAACCT GACCTGAAAC CTAAGGACAT CGTACATGAG 
AAGGATGTGA CGTCAAAGAA AGGAGTTCGT GTTGGAGAAA GAGACGACAC ACTCCCACCA 
GAATACAGAT TCAAAGAGGA AGATCTCAAG AAATATCCTC CTCCTCAGAA AGATCCTTCC 
TTAGATGCTA ATGAAGCTCT GGATATTCTG TCTGGGGATT TTACAACTCC TTCCGCACCA 
TCGGCTGTTT CCCCTGCCTC TAAATCCTCT GCCAAGTCTT CAGATTCAGC TTTAGATGCT 
CTTGCTGGTG ATTTCGTCGC TTCTTCTTCT GCGTCTTCAG TTCATTCTGC CGTCTCTGGC 
CCCCCACGCT CCGACAGACT GTTGTCAGAC GGAACCTCAT CAGCTCTAGA TGCCCTGTCA 
GAAACTCTGG GAGACATTAA AGCAGCCCCT GAACCCGCCC CTGTCCTACC TAAAACTGTC 
GTTAAGGAGA AGACGGTCAT AGAAGAGAGA GTGAGTAAAC CGGGTGAGAG AGACGACTCC 
CTTCCACCAG ATCAGCGGTT CACTGCGGAG GATCGCAAGG CATTTGAAGA AGCAAAGAAG 
AAAGACGCCA AACCAAAACA GCCATCAATG GACGATTCAG CGGCTCTGGA CATGTTGTCT 
AGTGATTTTT CAGATGTACC GACTGTAAAG CCCACTGTAT CTGATGGCAA AAGCTTCACC 
CCTGAACCAA CACCACCAAC CTTTAAGGCA ACCGGTCCGG TTTTAGACAA ACTGGCAGAC 
AACGTGCTTT CCAACCTGAC TGACCCCAAA GCCAAAGACA GCAAAGCAAA GGGAAAGGGG 
GCGAAACCAA AGTCTAAACC AAAGAAACAG GCAGTGGAAG ACTCTTCAGC CACAGATCTG 
CTGTCCAGTA AACCGAGCTC AGATGTGGTG CCTTCAGCTT CCACAAAGGC TGGAAAAAGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_005161049.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161106.1 Danio rerio calpastatin (cast), transcript variant X31, mRNA.

Price & Availability↑

CloneID	ODa25624
Clone ID Related Accession (Same CDS sequence)	XM_005161048.4 , XM_005161049.3 , XM_005161048.3 , XM_005161049.4
Accession Version	XM_005161049.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calpastatin isoform X31
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005161049.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC AGGTCACACC CACCACAACA TCTCAGGTCT CCACAGTGAA ACCTGCACAA 
TATGAGGTGC AGGTTGAAGT GGGTCCTTCA GCAGCAAAAG TCAGTGCAGA TGCTGACGAT 
CCGTTTGATG CCCTGTCTGG CACATTACCA TCATCACAAC CTTTGGCTCC TAAAGTCCAC 
TACACTGGAC CTGAGATCAA AGAGCCCAAA CTGAAATCAG AGAAAGGTGT TTTGTGTGGT 
GAGCGAGATG ACACGCTGCC GCCCGGATAC AGAAAGGAAG ACCTGGAAAA GAAGCATGGA 
GCTGGAGTTC CAGCTGGAGT TCCGGAGAAG CCTAAAGAAG TTCCCAAGCC CATAAGCACA 
GATGATGCAC TGGAATCTCT CTCCTCTACG TTTGTGTCTC CAGCTCAAGC TGCTCCAAAG 
AAGACAGACG TGAAAACTGA AACAATAGGA GCAGTGGATG TTAAATCGGC AGGCACCACT 
AACTTTGCTC CCCCTCCACC ATCTCAGCAG AAACAACCGG CGTCGTCTCA AGCTGCTGCT 
GTAACCAAAT CACCTGCTCC ACCGGCAGAC AAGAAAGCTA AAATGGAGAC TCATGCACAA 
CCAGCCAAAG CAAAGACAGA GTCGGACAGC TCCATATCGC TGGACGCTCT CGATGCTTTG 
AGCGACACAC TGGGGGCTTC TAAACCACCC CAAAAATTAC CAGAACTCAA ACCTGGACAG 
ATAGTTGATG AGAAGAAACA GACCTCAGAG AAAGGTGTTC GTGTTGGAGA AAGAGACGAC 
ACACTTCCAC CACAATACAG ATTCAAAGAG GAAGATCTCA AGAAATATCC TCCACCTGCG 
AAAGAGCCTT CACTGAACAC AGATGATGCG ATGGACATAC TTTCAGGAGA TTTCACGACC 
CCTGCATCGG CACCTGTTGT CAAAGCTCCT GTTCCTCCTG CACAGGACAC AAAGAAGCAT 
GCTGCAGTGC CTGAGAAGAC CAAAGATATT CCCAAGACTA AAGCTGATGC TCTGTCAAGT 
GATTTCGTGG CTCCGGCACA TTCTGCTCCT AAGGTTTCTT CAGCTGCTCC TAAAACCATC 
CCTCCAGGCC CACCACAAAC CCCAGATGAG GATCCTTTCA GTGCTTTGGG CGACACTCTT 
TCTGCCCCAG AACCACCAAA AAAACAACCT GACCTGAAAC CTAAGGACAT CGTACATGAG 
AAGGATGTGA CGTCAAAGAA AGGAGTTCGT GTTGGAGAAA GAGACGACAC ACTCCCACCA 
GAATACAGAT TCAAAGAGGA AGATCTCAAG AAATATCCTC CTCCTCAGAA AGATCCTTCC 
TTAGATGCTA ATGAAGCTCT GGATATTCTG TCTGGGGATT TTACAACTCC TTCCGCACCA 
TCGGCTGTTT CCCCTGCCTC TAAATCCTCT GCCAAGTCTT CAGATTCAGC TTTAGATGCT 
CTTGCTGGTG ATTTCGTCGC TTCTTCTTCT GCGTCTTCAG TTCATTCTGC CGTCTCTGGC 
CCCCCACGCT CCGACAGACT GTTGTCAGAC GGAACCTCAT CAGCTCTAGA TGCCCTGTCA 
GAAACTCTGG GAGACATTAA AGCAGCCCCT GAACCCGCCC CTGTCCTACC TAAAACTGTC 
GTTAAGGAGA AGACGGTCAT AGAAGAGAGA GTGAGTAAAC CGGGTGAGAG AGACGACTCC 
CTTCCACCAG ATCAGCGGTT CACTGCGGAG GATCGCAAGG CATTTGAAGA AGCAAAGAAG 
AAAGACGCCA AACCAAAACA GCCATCAATG GACGATTCAG CGGCTCTGGA CATGTTGTCT 
AGTGATTTTT CAGATGTACC GACTGTAAAG CCCACTGTAT CTGATGGCAA AAGCTTCACC 
CCTGAACCAA CACCACCAAC CTTTAAGGCA ACCGGTCCGG TTTTAGACAA ACTGGCAGAC 
AACGTGCTTT CCAACCTGAC TGACCCCAAA GCCAAAGACA GCAAAGCAAA GGGAAAGGGG 
GCGAAACCAA AGTCTAAACC AAAGAAACAG GCAGTGGAAG ACTCTTCAGC CACAGATCTG 
CTGTCCAGTA AACCGAGCTC AGATGTGGTG CCTTCAGCTT CCACAAAGGC TGGAAAAAGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161106.1
CDS	77..2179
Translation	MSQQVTPTTTSQVSTVKPAQYEVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_005161049.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161049.4

ORF Insert Sequence:

ATGTCTCAGC AGGTCACACC CACCACAACA TCTCAGGTCT CCACAGTGAA ACCTGCACAA 
TATGAGGTGC AGGTTGAAGT GGGTCCTTCA GCAGCAAAAG TCAGTGCAGA TGCTGACGAT 
CCGTTTGATG CCCTGTCTGG CACATTACCA TCATCACAAC CTTTGGCTCC TAAAGTCCAC 
TACACTGGAC CTGAGATCAA AGAGCCCAAA CTGAAATCAG AGAAAGGTGT TTTGTGTGGT 
GAGCGAGATG ACACGCTGCC GCCCGGATAC AGAAAGGAAG ACCTGGAAAA GAAGCATGGA 
GCTGGAGTTC CAGCTGGAGT TCCGGAGAAG CCTAAAGAAG TTCCCAAGCC CATAAGCACA 
GATGATGCAC TGGAATCTCT CTCCTCTACG TTTGTGTCTC CAGCTCAAGC TGCTCCAAAG 
AAGACAGACG TGAAAACTGA AACAATAGGA GCAGTGGATG TTAAATCGGC AGGCACCACT 
AACTTTGCTC CCCCTCCACC ATCTCAGCAG AAACAACCGG CGTCGTCTCA AGCTGCTGCT 
GTAACCAAAT CACCTGCTCC ACCGGCAGAC AAGAAAGCTA AAATGGAGAC TCATGCACAA 
CCAGCCAAAG CAAAGACAGA GTCGGACAGC TCCATATCGC TGGACGCTCT CGATGCTTTG 
AGCGACACAC TGGGGGCTTC TAAACCACCC CAAAAATTAC CAGAACTCAA ACCTGGACAG 
ATAGTTGATG AGAAGAAACA GACCTCAGAG AAAGGTGTTC GTGTTGGAGA AAGAGACGAC 
ACACTTCCAC CACAATACAG ATTCAAAGAG GAAGATCTCA AGAAATATCC TCCACCTGCG 
AAAGAGCCTT CACTGAACAC AGATGATGCG ATGGACATAC TTTCAGGAGA TTTCACGACC 
CCTGCATCGG CACCTGTTGT CAAAGCTCCT GTTCCTCCTG CACAGGACAC AAAGAAGCAT 
GCTGCAGTGC CTGAGAAGAC CAAAGATATT CCCAAGACTA AAGCTGATGC TCTGTCAAGT 
GATTTCGTGG CTCCGGCACA TTCTGCTCCT AAGGTTTCTT CAGCTGCTCC TAAAACCATC 
CCTCCAGGCC CACCACAAAC CCCAGATGAG GATCCTTTCA GTGCTTTGGG CGACACTCTT 
TCTGCCCCAG AACCACCAAA AAAACAACCT GACCTGAAAC CTAAGGACAT CGTACATGAG 
AAGGATGTGA CGTCAAAGAA AGGAGTTCGT GTTGGAGAAA GAGACGACAC ACTCCCACCA 
GAATACAGAT TCAAAGAGGA AGATCTCAAG AAATATCCTC CTCCTCAGAA AGATCCTTCC 
TTAGATGCTA ATGAAGCTCT GGATATTCTG TCTGGGGATT TTACAACTCC TTCCGCACCA 
TCGGCTGTTT CCCCTGCCTC TAAATCCTCT GCCAAGTCTT CAGATTCAGC TTTAGATGCT 
CTTGCTGGTG ATTTCGTCGC TTCTTCTTCT GCGTCTTCAG TTCATTCTGC CGTCTCTGGC 
CCCCCACGCT CCGACAGACT GTTGTCAGAC GGAACCTCAT CAGCTCTAGA TGCCCTGTCA 
GAAACTCTGG GAGACATTAA AGCAGCCCCT GAACCCGCCC CTGTCCTACC TAAAACTGTC 
GTTAAGGAGA AGACGGTCAT AGAAGAGAGA GTGAGTAAAC CGGGTGAGAG AGACGACTCC 
CTTCCACCAG ATCAGCGGTT CACTGCGGAG GATCGCAAGG CATTTGAAGA AGCAAAGAAG 
AAAGACGCCA AACCAAAACA GCCATCAATG GACGATTCAG CGGCTCTGGA CATGTTGTCT 
AGTGATTTTT CAGATGTACC GACTGTAAAG CCCACTGTAT CTGATGGCAA AAGCTTCACC 
CCTGAACCAA CACCACCAAC CTTTAAGGCA ACCGGTCCGG TTTTAGACAA ACTGGCAGAC 
AACGTGCTTT CCAACCTGAC TGACCCCAAA GCCAAAGACA GCAAAGCAAA GGGAAAGGGG 
GCGAAACCAA AGTCTAAACC AAAGAAACAG GCAGTGGAAG ACTCTTCAGC CACAGATCTG 
CTGTCCAGTA AACCGAGCTC AGATGTGGTG CCTTCAGCTT CCACAAAGGC TGGAAAAAGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_009295209.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293484.1 Danio rerio calpastatin (cast), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa43965
Clone ID Related Accession (Same CDS sequence)	XM_009295209.3 , XM_009295209.2
Accession Version	XM_009295209.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calpastatin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009295209.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGGTCTC GCTTACTGGA TATTATCCTA TCGAGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGAA AGGAGCTACA 
CAATCGTCTA CAGCAGCTGC GACTGTCAAA TCCGGCGCTG GAGATCCTGC AACCACCCAG 
AAAGGATCTT CTCAAGTCAA ACCTCAGGTC ACAGATTCTA AATCAACACC TGCATCTTCT 
GCTAAAGTCT CAGCTGTGAG CTCTGGGACT GGAGTTGCTG GTACAACCGG TGGAGCAGGA 
GTGAAACCAG CAGATCCTGC AAAAGATACG CCTCAGAGCA CCACCATTCC TTCAACCAAA 
CTGGGACCTG CTAAGGTGGA TCCGGCTGCT GGGAAGCCCT CTGGCTCAGC TGGTGCCCAA 
AAACAGACAA GTGCTCAAAA GGTGCAGGTT GAAGTGGGTC CTTCAGCAGC AAAAGTCAGT 
GCAGATGCTG ACGATCCGTT TGATGCCCTG TCTGGCACAT TACCATCATC ACAACCTTTG 
GCTCCTAAAG TCCACTACAC TGGACCTGAG ATCAAAGAGC CCAAACTGAA ATCAGAGAAA 
GGTGTTTTGT GTGGTGAGCG AGATGACACG CTGCCGCCCG GATACAGAAA GGAAGACCTG 
GAAAAGAAGC ATGGAGCTGG AGTTCCAGCT GGAGTTCCGG AGAAGCCTAA AGAAGTTCCC 
AAGCCCATAA GCACAGATGA TGCACTGGAA TCTCTCTCCT CTACGTTTGT GTCTCCAGCT 
CAAGCTGCTC CAAAGAAGAC AGACGTGAAA ACTGAAACAA TAGGAGCAGT GGATGTTAAA 
TCGGCAGGCA CCACTAACTT TGCTCCCCCT CCACCATCTC AGCAGAAACA ACCGGCGTCG 
TCTCAAGCTG CTGCTGTAAC CAAATCACCT GCTCCACCGG CAGACAAGAA AGCTAAAATG 
GAGACTCATG CACAACCAGC CAAAGCAAAG ACAGAGTCGG ACAGCTCCAT ATCGCTGGAC 
GCTCTCGATG CTTTGAGCGA CACACTGGGG GCTTCTAAAC CACCCCAAAA ATTACCAGAA 
CTCAAACCTG GACAGATAGT TGATGAGAAG AAACAGACCT CAGAGAAAGG TGTTCGTGTT 
GGAGAAAGAG ACGACACACT TCCACCACAA TACAGATTCA AAGAGGAAGA TCTCAAGAAA 
TATCCTCCAC CTGCGAAAGA GCCTTCACTG AACACAGATG ATGCGATGGA CATACTTTCA 
GGAGATTTCA CGACCCCTGC ATCGGCACCT GTTGTCAAAG CTCCTGTTCC TCCTGCACAG 
GACACAAAGA AGCATGCTGC AGTGCCTGAG AAGACCAAAG ATATTCCCAA GACTAAAGCT 
GATGCTCTGT CAAGTGATTT CGTGGCTCCG GCACATTCTG CTCCTAAGGT TTCTTCAGCT 
GCTCCTAAAA CCATCCCTCC AGGCCCACCA CAAACCCCAG ATGAGGATCC TTTCAGTGCT 
TTGGGCGACA CTCTTTCTGC CCCAGAACCA CCAAAAAAAC AACCTGACCT GAAACCTAAG 
GACATCGTAC ATGAGAAGGA TGTGACGTCA AAGAAAGGAG TTCGTGTTGG AGAAAGAGAC 
GACACACTCC CACCAGAATA CAGATTCAAA GAGGAAGATC TCAAGAAATA TCCTCCTCCT 
CAGAAAGATC CTTCCTTAGA TGCTAATGAA GCTCTGGATA TTCTGTCTGG GGATTTTACA 
ACTCCTTCCG CACCATCGGC TGTTTCCCCT GCCTCTAAAT CCTCTGCCAA GTCTTCAGAT 
TCAGCTTTAG ATGCTCTTGC TGGTGATTTC GTCGCTTCTT CTTCTGCGTC TTCAGTTCAT 
TCTGCCGTCT CTGGCCCCCC ACGCTCCGAC AGACTGTTGT CAGACGGAAC CTCATCAGCT 
CTAGATGCCC TGTCAGAAAC TCTGGGAGAC ATTAAAGCAG CCCCTGAACC CGCCCCTGTC 
CTACCTAAAA CTGTCGTTAA GGAGAAGACG GTCATAGAAG AGAGAGTGAG TAAACCGGGT 
GAGAGAGACG ACTCCCTTCC ACCAGATCAG CGGTTCACTG CGGAGGATCG CAAGGCATTT 
GAAGAAGCAA AGAAGAAAGA CGCCAAACCA AAACAGCCAT CAATGGACGA TTCAGCGGCT 
CTGGACATGT TGTCTAGTGA TTTTTCAGAT GTACCGACTG TAAAGCCCAC TGTATCTGAT 
GGCAAAAGCT TCACCCCTGA ACCAACACCA CCAACCTTTA AGGCAACCGG TCCGGTTTTA 
GACAAACTGG CAGACAACGT GCTTTCCAAC CTGACTGACC CCAAAGCCAA AGACAGCAAA 
GCAAAGGGAA AGGGGGCGAA ACCAAAGTCT AAACCAAAGA AACAGGCAGT GGAAGACTCT 
TCAGCCACAG ATCTGCTGTC CAGTAAACCG AGCTCAGATG TGGTGCCTTC AGCTTCCACA 
AAGGCTGGAA AAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293484.1
CDS	69..2606
Translation	MGQIFSWIRGTRDQPALQDIAVEQQVSLTGYYPIESQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQVTDSKSTPASSAKVSAVSSGTGVAGTTGGAGVKPADPAKDTPQSTTIPSTKLGPAKVDPAAGKPSGSAGAQKQTSAQKVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_009295209.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295209.3

ORF Insert Sequence:

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGGTCTC GCTTACTGGA TATTATCCTA TCGAGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGAA AGGAGCTACA 
CAATCGTCTA CAGCAGCTGC GACTGTCAAA TCCGGCGCTG GAGATCCTGC AACCACCCAG 
AAAGGATCTT CTCAAGTCAA ACCTCAGGTC ACAGATTCTA AATCAACACC TGCATCTTCT 
GCTAAAGTCT CAGCTGTGAG CTCTGGGACT GGAGTTGCTG GTACAACCGG TGGAGCAGGA 
GTGAAACCAG CAGATCCTGC AAAAGATACG CCTCAGAGCA CCACCATTCC TTCAACCAAA 
CTGGGACCTG CTAAGGTGGA TCCGGCTGCT GGGAAGCCCT CTGGCTCAGC TGGTGCCCAA 
AAACAGACAA GTGCTCAAAA GGTGCAGGTT GAAGTGGGTC CTTCAGCAGC AAAAGTCAGT 
GCAGATGCTG ACGATCCGTT TGATGCCCTG TCTGGCACAT TACCATCATC ACAACCTTTG 
GCTCCTAAAG TCCACTACAC TGGACCTGAG ATCAAAGAGC CCAAACTGAA ATCAGAGAAA 
GGTGTTTTGT GTGGTGAGCG AGATGACACG CTGCCGCCCG GATACAGAAA GGAAGACCTG 
GAAAAGAAGC ATGGAGCTGG AGTTCCAGCT GGAGTTCCGG AGAAGCCTAA AGAAGTTCCC 
AAGCCCATAA GCACAGATGA TGCACTGGAA TCTCTCTCCT CTACGTTTGT GTCTCCAGCT 
CAAGCTGCTC CAAAGAAGAC AGACGTGAAA ACTGAAACAA TAGGAGCAGT GGATGTTAAA 
TCGGCAGGCA CCACTAACTT TGCTCCCCCT CCACCATCTC AGCAGAAACA ACCGGCGTCG 
TCTCAAGCTG CTGCTGTAAC CAAATCACCT GCTCCACCGG CAGACAAGAA AGCTAAAATG 
GAGACTCATG CACAACCAGC CAAAGCAAAG ACAGAGTCGG ACAGCTCCAT ATCGCTGGAC 
GCTCTCGATG CTTTGAGCGA CACACTGGGG GCTTCTAAAC CACCCCAAAA ATTACCAGAA 
CTCAAACCTG GACAGATAGT TGATGAGAAG AAACAGACCT CAGAGAAAGG TGTTCGTGTT 
GGAGAAAGAG ACGACACACT TCCACCACAA TACAGATTCA AAGAGGAAGA TCTCAAGAAA 
TATCCTCCAC CTGCGAAAGA GCCTTCACTG AACACAGATG ATGCGATGGA CATACTTTCA 
GGAGATTTCA CGACCCCTGC ATCGGCACCT GTTGTCAAAG CTCCTGTTCC TCCTGCACAG 
GACACAAAGA AGCATGCTGC AGTGCCTGAG AAGACCAAAG ATATTCCCAA GACTAAAGCT 
GATGCTCTGT CAAGTGATTT CGTGGCTCCG GCACATTCTG CTCCTAAGGT TTCTTCAGCT 
GCTCCTAAAA CCATCCCTCC AGGCCCACCA CAAACCCCAG ATGAGGATCC TTTCAGTGCT 
TTGGGCGACA CTCTTTCTGC CCCAGAACCA CCAAAAAAAC AACCTGACCT GAAACCTAAG 
GACATCGTAC ATGAGAAGGA TGTGACGTCA AAGAAAGGAG TTCGTGTTGG AGAAAGAGAC 
GACACACTCC CACCAGAATA CAGATTCAAA GAGGAAGATC TCAAGAAATA TCCTCCTCCT 
CAGAAAGATC CTTCCTTAGA TGCTAATGAA GCTCTGGATA TTCTGTCTGG GGATTTTACA 
ACTCCTTCCG CACCATCGGC TGTTTCCCCT GCCTCTAAAT CCTCTGCCAA GTCTTCAGAT 
TCAGCTTTAG ATGCTCTTGC TGGTGATTTC GTCGCTTCTT CTTCTGCGTC TTCAGTTCAT 
TCTGCCGTCT CTGGCCCCCC ACGCTCCGAC AGACTGTTGT CAGACGGAAC CTCATCAGCT 
CTAGATGCCC TGTCAGAAAC TCTGGGAGAC ATTAAAGCAG CCCCTGAACC CGCCCCTGTC 
CTACCTAAAA CTGTCGTTAA GGAGAAGACG GTCATAGAAG AGAGAGTGAG TAAACCGGGT 
GAGAGAGACG ACTCCCTTCC ACCAGATCAG CGGTTCACTG CGGAGGATCG CAAGGCATTT 
GAAGAAGCAA AGAAGAAAGA CGCCAAACCA AAACAGCCAT CAATGGACGA TTCAGCGGCT 
CTGGACATGT TGTCTAGTGA TTTTTCAGAT GTACCGACTG TAAAGCCCAC TGTATCTGAT 
GGCAAAAGCT TCACCCCTGA ACCAACACCA CCAACCTTTA AGGCAACCGG TCCGGTTTTA 
GACAAACTGG CAGACAACGT GCTTTCCAAC CTGACTGACC CCAAAGCCAA AGACAGCAAA 
GCAAAGGGAA AGGGGGCGAA ACCAAAGTCT AAACCAAAGA AACAGGCAGT GGAAGACTCT 
TCAGCCACAG ATCTGCTGTC CAGTAAACCG AGCTCAGATG TGGTGCCTTC AGCTTCCACA 
AAGGCTGGAA AAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_009295209.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293484.1 Danio rerio calpastatin (cast), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa43965
Clone ID Related Accession (Same CDS sequence)	XM_009295209.3 , XM_009295209.2
Accession Version	XM_009295209.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	calpastatin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295209.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGGTCTC GCTTACTGGA TATTATCCTA TCGAGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGAA AGGAGCTACA 
CAATCGTCTA CAGCAGCTGC GACTGTCAAA TCCGGCGCTG GAGATCCTGC AACCACCCAG 
AAAGGATCTT CTCAAGTCAA ACCTCAGGTC ACAGATTCTA AATCAACACC TGCATCTTCT 
GCTAAAGTCT CAGCTGTGAG CTCTGGGACT GGAGTTGCTG GTACAACCGG TGGAGCAGGA 
GTGAAACCAG CAGATCCTGC AAAAGATACG CCTCAGAGCA CCACCATTCC TTCAACCAAA 
CTGGGACCTG CTAAGGTGGA TCCGGCTGCT GGGAAGCCCT CTGGCTCAGC TGGTGCCCAA 
AAACAGACAA GTGCTCAAAA GGTGCAGGTT GAAGTGGGTC CTTCAGCAGC AAAAGTCAGT 
GCAGATGCTG ACGATCCGTT TGATGCCCTG TCTGGCACAT TACCATCATC ACAACCTTTG 
GCTCCTAAAG TCCACTACAC TGGACCTGAG ATCAAAGAGC CCAAACTGAA ATCAGAGAAA 
GGTGTTTTGT GTGGTGAGCG AGATGACACG CTGCCGCCCG GATACAGAAA GGAAGACCTG 
GAAAAGAAGC ATGGAGCTGG AGTTCCAGCT GGAGTTCCGG AGAAGCCTAA AGAAGTTCCC 
AAGCCCATAA GCACAGATGA TGCACTGGAA TCTCTCTCCT CTACGTTTGT GTCTCCAGCT 
CAAGCTGCTC CAAAGAAGAC AGACGTGAAA ACTGAAACAA TAGGAGCAGT GGATGTTAAA 
TCGGCAGGCA CCACTAACTT TGCTCCCCCT CCACCATCTC AGCAGAAACA ACCGGCGTCG 
TCTCAAGCTG CTGCTGTAAC CAAATCACCT GCTCCACCGG CAGACAAGAA AGCTAAAATG 
GAGACTCATG CACAACCAGC CAAAGCAAAG ACAGAGTCGG ACAGCTCCAT ATCGCTGGAC 
GCTCTCGATG CTTTGAGCGA CACACTGGGG GCTTCTAAAC CACCCCAAAA ATTACCAGAA 
CTCAAACCTG GACAGATAGT TGATGAGAAG AAACAGACCT CAGAGAAAGG TGTTCGTGTT 
GGAGAAAGAG ACGACACACT TCCACCACAA TACAGATTCA AAGAGGAAGA TCTCAAGAAA 
TATCCTCCAC CTGCGAAAGA GCCTTCACTG AACACAGATG ATGCGATGGA CATACTTTCA 
GGAGATTTCA CGACCCCTGC ATCGGCACCT GTTGTCAAAG CTCCTGTTCC TCCTGCACAG 
GACACAAAGA AGCATGCTGC AGTGCCTGAG AAGACCAAAG ATATTCCCAA GACTAAAGCT 
GATGCTCTGT CAAGTGATTT CGTGGCTCCG GCACATTCTG CTCCTAAGGT TTCTTCAGCT 
GCTCCTAAAA CCATCCCTCC AGGCCCACCA CAAACCCCAG ATGAGGATCC TTTCAGTGCT 
TTGGGCGACA CTCTTTCTGC CCCAGAACCA CCAAAAAAAC AACCTGACCT GAAACCTAAG 
GACATCGTAC ATGAGAAGGA TGTGACGTCA AAGAAAGGAG TTCGTGTTGG AGAAAGAGAC 
GACACACTCC CACCAGAATA CAGATTCAAA GAGGAAGATC TCAAGAAATA TCCTCCTCCT 
CAGAAAGATC CTTCCTTAGA TGCTAATGAA GCTCTGGATA TTCTGTCTGG GGATTTTACA 
ACTCCTTCCG CACCATCGGC TGTTTCCCCT GCCTCTAAAT CCTCTGCCAA GTCTTCAGAT 
TCAGCTTTAG ATGCTCTTGC TGGTGATTTC GTCGCTTCTT CTTCTGCGTC TTCAGTTCAT 
TCTGCCGTCT CTGGCCCCCC ACGCTCCGAC AGACTGTTGT CAGACGGAAC CTCATCAGCT 
CTAGATGCCC TGTCAGAAAC TCTGGGAGAC ATTAAAGCAG CCCCTGAACC CGCCCCTGTC 
CTACCTAAAA CTGTCGTTAA GGAGAAGACG GTCATAGAAG AGAGAGTGAG TAAACCGGGT 
GAGAGAGACG ACTCCCTTCC ACCAGATCAG CGGTTCACTG CGGAGGATCG CAAGGCATTT 
GAAGAAGCAA AGAAGAAAGA CGCCAAACCA AAACAGCCAT CAATGGACGA TTCAGCGGCT 
CTGGACATGT TGTCTAGTGA TTTTTCAGAT GTACCGACTG TAAAGCCCAC TGTATCTGAT 
GGCAAAAGCT TCACCCCTGA ACCAACACCA CCAACCTTTA AGGCAACCGG TCCGGTTTTA 
GACAAACTGG CAGACAACGT GCTTTCCAAC CTGACTGACC CCAAAGCCAA AGACAGCAAA 
GCAAAGGGAA AGGGGGCGAA ACCAAAGTCT AAACCAAAGA AACAGGCAGT GGAAGACTCT 
TCAGCCACAG ATCTGCTGTC CAGTAAACCG AGCTCAGATG TGGTGCCTTC AGCTTCCACA 
AAGGCTGGAA AAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293484.1
CDS	68..2605
Translation	MGQIFSWIRGTRDQPALQDIAVEQQVSLTGYYPIESQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQVTDSKSTPASSAKVSAVSSGTGVAGTTGGAGVKPADPAKDTPQSTTIPSTKLGPAKVDPAAGKPSGSAGAQKQTSAQKVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_009295209.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295209.2

ORF Insert Sequence:

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGGTCTC GCTTACTGGA TATTATCCTA TCGAGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGAA AGGAGCTACA 
CAATCGTCTA CAGCAGCTGC GACTGTCAAA TCCGGCGCTG GAGATCCTGC AACCACCCAG 
AAAGGATCTT CTCAAGTCAA ACCTCAGGTC ACAGATTCTA AATCAACACC TGCATCTTCT 
GCTAAAGTCT CAGCTGTGAG CTCTGGGACT GGAGTTGCTG GTACAACCGG TGGAGCAGGA 
GTGAAACCAG CAGATCCTGC AAAAGATACG CCTCAGAGCA CCACCATTCC TTCAACCAAA 
CTGGGACCTG CTAAGGTGGA TCCGGCTGCT GGGAAGCCCT CTGGCTCAGC TGGTGCCCAA 
AAACAGACAA GTGCTCAAAA GGTGCAGGTT GAAGTGGGTC CTTCAGCAGC AAAAGTCAGT 
GCAGATGCTG ACGATCCGTT TGATGCCCTG TCTGGCACAT TACCATCATC ACAACCTTTG 
GCTCCTAAAG TCCACTACAC TGGACCTGAG ATCAAAGAGC CCAAACTGAA ATCAGAGAAA 
GGTGTTTTGT GTGGTGAGCG AGATGACACG CTGCCGCCCG GATACAGAAA GGAAGACCTG 
GAAAAGAAGC ATGGAGCTGG AGTTCCAGCT GGAGTTCCGG AGAAGCCTAA AGAAGTTCCC 
AAGCCCATAA GCACAGATGA TGCACTGGAA TCTCTCTCCT CTACGTTTGT GTCTCCAGCT 
CAAGCTGCTC CAAAGAAGAC AGACGTGAAA ACTGAAACAA TAGGAGCAGT GGATGTTAAA 
TCGGCAGGCA CCACTAACTT TGCTCCCCCT CCACCATCTC AGCAGAAACA ACCGGCGTCG 
TCTCAAGCTG CTGCTGTAAC CAAATCACCT GCTCCACCGG CAGACAAGAA AGCTAAAATG 
GAGACTCATG CACAACCAGC CAAAGCAAAG ACAGAGTCGG ACAGCTCCAT ATCGCTGGAC 
GCTCTCGATG CTTTGAGCGA CACACTGGGG GCTTCTAAAC CACCCCAAAA ATTACCAGAA 
CTCAAACCTG GACAGATAGT TGATGAGAAG AAACAGACCT CAGAGAAAGG TGTTCGTGTT 
GGAGAAAGAG ACGACACACT TCCACCACAA TACAGATTCA AAGAGGAAGA TCTCAAGAAA 
TATCCTCCAC CTGCGAAAGA GCCTTCACTG AACACAGATG ATGCGATGGA CATACTTTCA 
GGAGATTTCA CGACCCCTGC ATCGGCACCT GTTGTCAAAG CTCCTGTTCC TCCTGCACAG 
GACACAAAGA AGCATGCTGC AGTGCCTGAG AAGACCAAAG ATATTCCCAA GACTAAAGCT 
GATGCTCTGT CAAGTGATTT CGTGGCTCCG GCACATTCTG CTCCTAAGGT TTCTTCAGCT 
GCTCCTAAAA CCATCCCTCC AGGCCCACCA CAAACCCCAG ATGAGGATCC TTTCAGTGCT 
TTGGGCGACA CTCTTTCTGC CCCAGAACCA CCAAAAAAAC AACCTGACCT GAAACCTAAG 
GACATCGTAC ATGAGAAGGA TGTGACGTCA AAGAAAGGAG TTCGTGTTGG AGAAAGAGAC 
GACACACTCC CACCAGAATA CAGATTCAAA GAGGAAGATC TCAAGAAATA TCCTCCTCCT 
CAGAAAGATC CTTCCTTAGA TGCTAATGAA GCTCTGGATA TTCTGTCTGG GGATTTTACA 
ACTCCTTCCG CACCATCGGC TGTTTCCCCT GCCTCTAAAT CCTCTGCCAA GTCTTCAGAT 
TCAGCTTTAG ATGCTCTTGC TGGTGATTTC GTCGCTTCTT CTTCTGCGTC TTCAGTTCAT 
TCTGCCGTCT CTGGCCCCCC ACGCTCCGAC AGACTGTTGT CAGACGGAAC CTCATCAGCT 
CTAGATGCCC TGTCAGAAAC TCTGGGAGAC ATTAAAGCAG CCCCTGAACC CGCCCCTGTC 
CTACCTAAAA CTGTCGTTAA GGAGAAGACG GTCATAGAAG AGAGAGTGAG TAAACCGGGT 
GAGAGAGACG ACTCCCTTCC ACCAGATCAG CGGTTCACTG CGGAGGATCG CAAGGCATTT 
GAAGAAGCAA AGAAGAAAGA CGCCAAACCA AAACAGCCAT CAATGGACGA TTCAGCGGCT 
CTGGACATGT TGTCTAGTGA TTTTTCAGAT GTACCGACTG TAAAGCCCAC TGTATCTGAT 
GGCAAAAGCT TCACCCCTGA ACCAACACCA CCAACCTTTA AGGCAACCGG TCCGGTTTTA 
GACAAACTGG CAGACAACGT GCTTTCCAAC CTGACTGACC CCAAAGCCAA AGACAGCAAA 
GCAAAGGGAA AGGGGGCGAA ACCAAAGTCT AAACCAAAGA AACAGGCAGT GGAAGACTCT 
TCAGCCACAG ATCTGCTGTC CAGTAAACCG AGCTCAGATG TGGTGCCTTC AGCTTCCACA 
AAGGCTGGAA AAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_021468868.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324543.1 Danio rerio calpastatin (cast), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa150920
Clone ID Related Accession (Same CDS sequence)	XM_021468868.1
Accession Version	XM_021468868.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calpastatin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGGTCTC GCTTACTGGA TATTATCCTA TCGAGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGAA AGGAGCTACA 
CAATCGTCTA CAGCAGCTGC GACTGTCAAA TCCGGCGCTG GAGATCCTGC AACCACCCAG 
AAAGGATCTT CTCAAGTCAA ACCTCAGGTC ACAGATTCTA AATCAACACC TGCATCTTCT 
GCTAAAGTCT CAGCTGTGAG CTCTGGGACT GGAGTTGCTG GTACAACCGG TGGAGCAGGA 
GTGAAACCAG CAGATCCTGC AAAAGATACG CCTCAGAGCA CCACCATTCC TTCAACCAAA 
CTGGGACCTG CTAAGGTGGA TCCGGCTGCT GGGAAGCCCT CTGGCTCAGC TGGTGCCCAA 
AAACAGACAA GTGCTCAAAA GGTGCAGGTT GAAGTGGGTC CTTCAGCAGC AAAAGCTGAC 
GATCCGTTTG ATGCCCTGTC TGGCACATTA CCATCATCAC AACCTTTGGC TCCTAAAGTC 
CACTACACTG GACCTGAGAT CAAAGAGCCC AAACTGAAAT CAGAGAAAGG TGTTTTGTGT 
GGTGAGCGAG ATGACACGCT GCCGCCCGGA TACAGAAAGG AAGACCTGGA AAAGAAGCAT 
GGAGCTGGAG TTCCAGCTGG AGTTCCGGAG AAGCCTAAAG AAGTTCCCAA GCCCATAAGC 
ACAGATGATG CACTGGAATC TCTCTCCTCT ACGTTTGTGT CTCCAGCTCA AGCTGCTCCA 
AAGAAGACAG ACGTGAAAAC TGAAACAATA GGAGCAGTGG ATGTTAAATC GGCAGGCACC 
ACTAACTTTG CTCCCCCTCC ACCATCTCAG CAGAAACAAC CGGCGTCGTC TCAAGCTGCT 
GCTGTAACCA AATCACCTGC TCCACCGGCA GACAAGAAAG CTAAAATGGA GACTCATGCA 
CAACCAGCCA AAGCAAAGAC AGAGTCGGAC AGCTCCATAT CGCTGGACGC TCTCGATGCT 
TTGAGCGACA CACTGGGGGC TTCTAAACCA CCCCAAAAAT TACCAGAACT CAAACCTGGA 
CAGATAGTTG ATGAGAAGAA ACAGACCTCA GAGAAAGGTG TTCGTGTTGG AGAAAGAGAC 
GACACACTTC CACCACAATA CAGATTCAAA GAGGAAGATC TCAAGAAATA TCCTCCACCT 
GCGAAAGAGC CTTCACTGAA CACAGATGAT GCGATGGACA TACTTTCAGG AGATTTCACG 
ACCCCTGCAT CGGCACCTGT TGTCAAAGCT CCTGTTCCTC CTGCACAGGA CACAAAGAAG 
CATGCTGCAG TGCCTGAGAA GACCAAAGAT ATTCCCAAGA CTAAAGCTGA TGCTCTGTCA 
AGTGATTTCG TGGCTCCGGC ACATTCTGCT CCTAAGGTTT CTTCAGCTGC TCCTAAAACC 
ATCCCTCCAG GCCCACCACA AACCCCAGAT GAGGATCCTT TCAGTGCTTT GGGCGACACT 
CTTTCTGCCC CAGAACCACC AAAAAAACAA CCTGACCTGA AACCTAAGGA CATCGTACAT 
GAGAAGGATG TGACGTCAAA GAAAGGAGTT CGTGTTGGAG AAAGAGACGA CACACTCCCA 
CCAGAATACA GATTCAAAGA GGAAGATCTC AAGAAATATC CTCCTCCTCA GAAAGATCCT 
TCCTTAGATG CTAATGAAGC TCTGGATATT CTGTCTGGGG ATTTTACAAC TCCTTCCGCA 
CCATCGGCTG TTTCCCCTGC CTCTAAATCC TCTGCCAAGT CTTCAGATTC AGCTTTAGAT 
GCTCTTGCTG GTGATTTCGT CGCTTCTTCT TCTGCGTCTT CAGTTCATTC TGCCGTCTCT 
GGCCCCCCAC GCTCCGACAG ACTGTTGTCA GACGGAACCT CATCAGCTCT AGATGCCCTG 
TCAGAAACTC TGGGAGACAT TAAAGCAGCC CCTGAACCCG CCCCTGTCCT ACCTAAAACT 
GTCGTTAAGG AGAAGACGGT CATAGAAGAG AGAGTGAGTA AACCGGGTGA GAGAGACGAC 
TCCCTTCCAC CAGATCAGCG GTTCACTGCG GAGGATCGCA AGGCATTTGA AGAAGCAAAG 
AAGAAAGACG CCAAACCAAA ACAGCCATCA ATGGACGATT CAGCGGCTCT GGACATGTTG 
TCTAGTGATT TTTCAGATGT ACCGACTGTA AAGCCCACTG TATCTGATGG CAAAAGCTTC 
ACCCCTGAAC CAACACCACC AACCTTTAAG GCAACCGGTC CGGTTTTAGA CAAACTGGCA 
GACAACGTGC TTTCCAACCT GACTGACCCC AAAGCCAAAG ACAGCAAAGC AAAGGGAAAG 
GGGGCGAAAC CAAAGTCTAA ACCAAAGAAA CAGGCAGTGG AAGACTCTTC AGCCACAGAT 
CTGCTGTCCA GTAAACCGAG CTCAGATGTG GTGCCTTCAG CTTCCACAAA GGCTGGAAAA 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324543.1
CDS	69..2594
Translation	MGQIFSWIRGTRDQPALQDIAVEQQVSLTGYYPIESQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQVTDSKSTPASSAKVSAVSSGTGVAGTTGGAGVKPADPAKDTPQSTTIPSTKLGPAKVDPAAGKPSGSAGAQKQTSAQKVQVEVGPSAAKADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_021468868.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468868.1

ORF Insert Sequence:

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGGTCTC GCTTACTGGA TATTATCCTA TCGAGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGAA AGGAGCTACA 
CAATCGTCTA CAGCAGCTGC GACTGTCAAA TCCGGCGCTG GAGATCCTGC AACCACCCAG 
AAAGGATCTT CTCAAGTCAA ACCTCAGGTC ACAGATTCTA AATCAACACC TGCATCTTCT 
GCTAAAGTCT CAGCTGTGAG CTCTGGGACT GGAGTTGCTG GTACAACCGG TGGAGCAGGA 
GTGAAACCAG CAGATCCTGC AAAAGATACG CCTCAGAGCA CCACCATTCC TTCAACCAAA 
CTGGGACCTG CTAAGGTGGA TCCGGCTGCT GGGAAGCCCT CTGGCTCAGC TGGTGCCCAA 
AAACAGACAA GTGCTCAAAA GGTGCAGGTT GAAGTGGGTC CTTCAGCAGC AAAAGCTGAC 
GATCCGTTTG ATGCCCTGTC TGGCACATTA CCATCATCAC AACCTTTGGC TCCTAAAGTC 
CACTACACTG GACCTGAGAT CAAAGAGCCC AAACTGAAAT CAGAGAAAGG TGTTTTGTGT 
GGTGAGCGAG ATGACACGCT GCCGCCCGGA TACAGAAAGG AAGACCTGGA AAAGAAGCAT 
GGAGCTGGAG TTCCAGCTGG AGTTCCGGAG AAGCCTAAAG AAGTTCCCAA GCCCATAAGC 
ACAGATGATG CACTGGAATC TCTCTCCTCT ACGTTTGTGT CTCCAGCTCA AGCTGCTCCA 
AAGAAGACAG ACGTGAAAAC TGAAACAATA GGAGCAGTGG ATGTTAAATC GGCAGGCACC 
ACTAACTTTG CTCCCCCTCC ACCATCTCAG CAGAAACAAC CGGCGTCGTC TCAAGCTGCT 
GCTGTAACCA AATCACCTGC TCCACCGGCA GACAAGAAAG CTAAAATGGA GACTCATGCA 
CAACCAGCCA AAGCAAAGAC AGAGTCGGAC AGCTCCATAT CGCTGGACGC TCTCGATGCT 
TTGAGCGACA CACTGGGGGC TTCTAAACCA CCCCAAAAAT TACCAGAACT CAAACCTGGA 
CAGATAGTTG ATGAGAAGAA ACAGACCTCA GAGAAAGGTG TTCGTGTTGG AGAAAGAGAC 
GACACACTTC CACCACAATA CAGATTCAAA GAGGAAGATC TCAAGAAATA TCCTCCACCT 
GCGAAAGAGC CTTCACTGAA CACAGATGAT GCGATGGACA TACTTTCAGG AGATTTCACG 
ACCCCTGCAT CGGCACCTGT TGTCAAAGCT CCTGTTCCTC CTGCACAGGA CACAAAGAAG 
CATGCTGCAG TGCCTGAGAA GACCAAAGAT ATTCCCAAGA CTAAAGCTGA TGCTCTGTCA 
AGTGATTTCG TGGCTCCGGC ACATTCTGCT CCTAAGGTTT CTTCAGCTGC TCCTAAAACC 
ATCCCTCCAG GCCCACCACA AACCCCAGAT GAGGATCCTT TCAGTGCTTT GGGCGACACT 
CTTTCTGCCC CAGAACCACC AAAAAAACAA CCTGACCTGA AACCTAAGGA CATCGTACAT 
GAGAAGGATG TGACGTCAAA GAAAGGAGTT CGTGTTGGAG AAAGAGACGA CACACTCCCA 
CCAGAATACA GATTCAAAGA GGAAGATCTC AAGAAATATC CTCCTCCTCA GAAAGATCCT 
TCCTTAGATG CTAATGAAGC TCTGGATATT CTGTCTGGGG ATTTTACAAC TCCTTCCGCA 
CCATCGGCTG TTTCCCCTGC CTCTAAATCC TCTGCCAAGT CTTCAGATTC AGCTTTAGAT 
GCTCTTGCTG GTGATTTCGT CGCTTCTTCT TCTGCGTCTT CAGTTCATTC TGCCGTCTCT 
GGCCCCCCAC GCTCCGACAG ACTGTTGTCA GACGGAACCT CATCAGCTCT AGATGCCCTG 
TCAGAAACTC TGGGAGACAT TAAAGCAGCC CCTGAACCCG CCCCTGTCCT ACCTAAAACT 
GTCGTTAAGG AGAAGACGGT CATAGAAGAG AGAGTGAGTA AACCGGGTGA GAGAGACGAC 
TCCCTTCCAC CAGATCAGCG GTTCACTGCG GAGGATCGCA AGGCATTTGA AGAAGCAAAG 
AAGAAAGACG CCAAACCAAA ACAGCCATCA ATGGACGATT CAGCGGCTCT GGACATGTTG 
TCTAGTGATT TTTCAGATGT ACCGACTGTA AAGCCCACTG TATCTGATGG CAAAAGCTTC 
ACCCCTGAAC CAACACCACC AACCTTTAAG GCAACCGGTC CGGTTTTAGA CAAACTGGCA 
GACAACGTGC TTTCCAACCT GACTGACCCC AAAGCCAAAG ACAGCAAAGC AAAGGGAAAG 
GGGGCGAAAC CAAAGTCTAA ACCAAAGAAA CAGGCAGTGG AAGACTCTTC AGCCACAGAT 
CTGCTGTCCA GTAAACCGAG CTCAGATGTG GTGCCTTCAG CTTCCACAAA GGCTGGAAAA 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_021468869.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324544.1 Danio rerio calpastatin (cast), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ODa150921
Clone ID Related Accession (Same CDS sequence)	XM_021468869.1
Accession Version	XM_021468869.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calpastatin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGGTCTC GCTTACTGGA TATTATCCTA TCGAGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGAA AGGAGCTACA 
CAATCGTCTA CAGCAGCTGC GACTGTCAAA TCCGGCGCTG GAGATCCTGC AACCACCCAG 
AAAGGATCTT CTCAAGTCAA ACCTCAGGTC ACAGATTCTA AATCAACACC TGCATCTTCT 
GCTAAAGTCT CAGCTGTGAG CTCTGGGACT GGAGTTGCTG GTACAACCGG TGGAGCAGGA 
GTGAAACCAG CAGATCCTGC AAAAGATACG CCTCAGAGCA CCACCATTCC TTCAACCAAA 
CTGGGACCTG CTAAGGTGGA TCCGGCTGCT GGGAAGCCCT CTGGCTCAGC TGGTGCCCAA 
AAACAGACAA GTGCTCAAAA GGTGCAGGTT GAAGTGGGTC CTTCAGCAGC AAAAGTCAGT 
GCAGATGCTG ACGATCCGTT TGATGCCCTG TCTGGCACAT TACCATCATC ACAACCTTTG 
GCTCCTAAAG TCCACTACAC TGGACCTGAG ATCAAAGAGC CCAAACTGAA ATCAGAGAAA 
GGTGTTTTGT GTGGTGAGCG AGATGACACG CTGCCGCCCG GATACAGAAA GGAAGACCTG 
GAAAAGAAGC ATGGAGCTGG AGTTCCAGCT GGAGTTCCGG AGAAGCCTAA AGAAGTTCCC 
AAGCCCATAA GCACAGATGA TGCACTGGAA TCTCTCTCCT CTACGTTTGT GTCTCCAGCT 
CAAGCTGCTC CAAAGAAGAC AGACGTGAAA ACTGAAACAA TAGGAGCAGT GGATGTTAAA 
TCGGCAGGCA CCACTAACTT TGCTCCCCCT CCACCATCTC AGCAGAAACA ACCGGCGTCG 
TCTCAAGCTG CTGCTGTAAC CAAATCACCT GCTCCACCGG CAGACAAGAA AGCTAAAATG 
GAGACTCATG CACAACCAGC CAAAGCAAAG ACAGAGTCGG ACAGCTCCAT ATCGCTGGAC 
GCTCTCGATG CTTTGAGCGA CACACTGGGG GCTTCTAAAC CACCCCAAAA ATTACCAGAA 
CTCAAACCTG GACAGATAGT TGATGAGAAG AAACAGACCT CAGAGAAAGG TGTTCGTGTT 
GGAGAAAGAG ACGACACACT TCCACCACAA TACAGATTCA AAGAGGAAGA TCTCAAGAAA 
TATCCTCCAC CTGCGAAAGA GCCTTCACTG AACACAGATG ATGCGATGGA CATACTTTCA 
GGAGATTTCA CGACCCCTGC ATCGGCACCT GTTGTCAAAG CTCCTGTTCC TCCTGCACAG 
GACACAAAGA AGCATGCTGC AGTGCCTGAG AAGACCAAAG ATATTCCCAA GACTAAAGCT 
GATGCTCTGT CAAGTGATTT CGTGGCTCCG GCACATTCTG CTCCTAAGGT TTCTTCAGCT 
GCTCCTAAAA CCATCCCTCC AGGCCCACCA CAAACCCCAG ATGAGGATCC TTTCAGTGCT 
TTGGGCGACA CTCTTTCTGC CCCAGAACCA CCAAAAAAAC AACCTGACCT GAAACCTAAG 
GACATCGTAC ATGAGAAGGA TGTGACGTCA AAGAAAGGAG TTCGTGTTGG AGAAAGAGAC 
GACACACTCC CACCAGAATA CAGATTCAAA GAGGAAGATC TCAAGAAATA TCCTCCTCCT 
CAGAAAGATC CTTCCTTAGA TGCTAATGAA GCTCTGGATA TTCTGTCTGG GGATTTTACA 
ACTCCTTCCG CACCATCGGC TGTTTCCCCT GCCTCTAAAT CCTCTGCCAA GTCTTCAGAT 
TCAGCTTTAG ATGCTCTTGC TGGTGATTTC GTCGCTTCTT CTTCTGCGTC TTCAGTTCAT 
TCTGCCGTCT CTGGCCCCCC ACGCTCCGAC AGACTGTTGT CAGACGGAAC CTCATCAGCT 
CTAGATGCCC TGTCAGAAAC TCTGGGAGAC ATTAAAGCAG CCCCTGAACC CGCCCCTGTC 
CTACCTAAAA CTGTCGTTAA GGAGAAGACG GTCATAGAAG AGAGAGTGAG TAAACCGGGT 
GAGAGAGACG ACTCCCTTCC ACCAGATCAG CGGTTCACTG CGGAGGATCG CAAGGCATTT 
GAAGAAGCAA AGAAGAAAGA CGCCAAACCA AAACAGCCAT CAATGGACGA TTCAGCGGCT 
CTGGACATGT TGTCTAGTGA TTTTTCAGAT GTACCGACTG TAAAGCCCAC TGTATCTGAT 
GGCAAAAGCT TCACCCCTGA ACCAACACCA CCAACCTTTA AGGCAACCGG TCCGGTTTTA 
GACAAACTGG CAGACAACGT GCTTTCCAAC CTGACTGACC CCAAAGCCAA AGACAGCAAA 
GCAAAGAAAC AGGCAGTGGA AGACTCTTCA GCCACAGATC TGCTGTCCAG TAAACCGAGC 
TCAGATGTGG TGCCTTCAGC TTCCACAAAG GCTGGAAAAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324544.1
CDS	69..2573
Translation	MGQIFSWIRGTRDQPALQDIAVEQQVSLTGYYPIESQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQVTDSKSTPASSAKVSAVSSGTGVAGTTGGAGVKPADPAKDTPQSTTIPSTKLGPAKVDPAAGKPSGSAGAQKQTSAQKVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_021468869.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468869.1

ORF Insert Sequence:

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGGTCTC GCTTACTGGA TATTATCCTA TCGAGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGAA AGGAGCTACA 
CAATCGTCTA CAGCAGCTGC GACTGTCAAA TCCGGCGCTG GAGATCCTGC AACCACCCAG 
AAAGGATCTT CTCAAGTCAA ACCTCAGGTC ACAGATTCTA AATCAACACC TGCATCTTCT 
GCTAAAGTCT CAGCTGTGAG CTCTGGGACT GGAGTTGCTG GTACAACCGG TGGAGCAGGA 
GTGAAACCAG CAGATCCTGC AAAAGATACG CCTCAGAGCA CCACCATTCC TTCAACCAAA 
CTGGGACCTG CTAAGGTGGA TCCGGCTGCT GGGAAGCCCT CTGGCTCAGC TGGTGCCCAA 
AAACAGACAA GTGCTCAAAA GGTGCAGGTT GAAGTGGGTC CTTCAGCAGC AAAAGTCAGT 
GCAGATGCTG ACGATCCGTT TGATGCCCTG TCTGGCACAT TACCATCATC ACAACCTTTG 
GCTCCTAAAG TCCACTACAC TGGACCTGAG ATCAAAGAGC CCAAACTGAA ATCAGAGAAA 
GGTGTTTTGT GTGGTGAGCG AGATGACACG CTGCCGCCCG GATACAGAAA GGAAGACCTG 
GAAAAGAAGC ATGGAGCTGG AGTTCCAGCT GGAGTTCCGG AGAAGCCTAA AGAAGTTCCC 
AAGCCCATAA GCACAGATGA TGCACTGGAA TCTCTCTCCT CTACGTTTGT GTCTCCAGCT 
CAAGCTGCTC CAAAGAAGAC AGACGTGAAA ACTGAAACAA TAGGAGCAGT GGATGTTAAA 
TCGGCAGGCA CCACTAACTT TGCTCCCCCT CCACCATCTC AGCAGAAACA ACCGGCGTCG 
TCTCAAGCTG CTGCTGTAAC CAAATCACCT GCTCCACCGG CAGACAAGAA AGCTAAAATG 
GAGACTCATG CACAACCAGC CAAAGCAAAG ACAGAGTCGG ACAGCTCCAT ATCGCTGGAC 
GCTCTCGATG CTTTGAGCGA CACACTGGGG GCTTCTAAAC CACCCCAAAA ATTACCAGAA 
CTCAAACCTG GACAGATAGT TGATGAGAAG AAACAGACCT CAGAGAAAGG TGTTCGTGTT 
GGAGAAAGAG ACGACACACT TCCACCACAA TACAGATTCA AAGAGGAAGA TCTCAAGAAA 
TATCCTCCAC CTGCGAAAGA GCCTTCACTG AACACAGATG ATGCGATGGA CATACTTTCA 
GGAGATTTCA CGACCCCTGC ATCGGCACCT GTTGTCAAAG CTCCTGTTCC TCCTGCACAG 
GACACAAAGA AGCATGCTGC AGTGCCTGAG AAGACCAAAG ATATTCCCAA GACTAAAGCT 
GATGCTCTGT CAAGTGATTT CGTGGCTCCG GCACATTCTG CTCCTAAGGT TTCTTCAGCT 
GCTCCTAAAA CCATCCCTCC AGGCCCACCA CAAACCCCAG ATGAGGATCC TTTCAGTGCT 
TTGGGCGACA CTCTTTCTGC CCCAGAACCA CCAAAAAAAC AACCTGACCT GAAACCTAAG 
GACATCGTAC ATGAGAAGGA TGTGACGTCA AAGAAAGGAG TTCGTGTTGG AGAAAGAGAC 
GACACACTCC CACCAGAATA CAGATTCAAA GAGGAAGATC TCAAGAAATA TCCTCCTCCT 
CAGAAAGATC CTTCCTTAGA TGCTAATGAA GCTCTGGATA TTCTGTCTGG GGATTTTACA 
ACTCCTTCCG CACCATCGGC TGTTTCCCCT GCCTCTAAAT CCTCTGCCAA GTCTTCAGAT 
TCAGCTTTAG ATGCTCTTGC TGGTGATTTC GTCGCTTCTT CTTCTGCGTC TTCAGTTCAT 
TCTGCCGTCT CTGGCCCCCC ACGCTCCGAC AGACTGTTGT CAGACGGAAC CTCATCAGCT 
CTAGATGCCC TGTCAGAAAC TCTGGGAGAC ATTAAAGCAG CCCCTGAACC CGCCCCTGTC 
CTACCTAAAA CTGTCGTTAA GGAGAAGACG GTCATAGAAG AGAGAGTGAG TAAACCGGGT 
GAGAGAGACG ACTCCCTTCC ACCAGATCAG CGGTTCACTG CGGAGGATCG CAAGGCATTT 
GAAGAAGCAA AGAAGAAAGA CGCCAAACCA AAACAGCCAT CAATGGACGA TTCAGCGGCT 
CTGGACATGT TGTCTAGTGA TTTTTCAGAT GTACCGACTG TAAAGCCCAC TGTATCTGAT 
GGCAAAAGCT TCACCCCTGA ACCAACACCA CCAACCTTTA AGGCAACCGG TCCGGTTTTA 
GACAAACTGG CAGACAACGT GCTTTCCAAC CTGACTGACC CCAAAGCCAA AGACAGCAAA 
GCAAAGAAAC AGGCAGTGGA AGACTCTTCA GCCACAGATC TGCTGTCCAG TAAACCGAGC 
TCAGATGTGG TGCCTTCAGC TTCCACAAAG GCTGGAAAAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_021468870.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324545.1 Danio rerio calpastatin (cast), transcript variant X10, mRNA.

Price & Availability↑

CloneID	ODa150922
Clone ID Related Accession (Same CDS sequence)	XM_021468870.1
Accession Version	XM_021468870.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calpastatin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGGTCTC GCTTACTGGA TATTATCCTA TCGAGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGAA AGGAGCTACA 
CAATCGTCTA CAGCAGCTGC GACTGTCAAA TCCGGCGCTG GAGATCCTGC AACCACCCAG 
AAAGGATCTT CTCAAGTCAA ACCTCAGGTC ACAGATTCTA AATCAACACC TGCATCTTCT 
GCTAAAGTCT CAGCTGTGAG CTCTGGGACT GGAGTTGCTG GTACAACCGG TGGAGCAGGA 
GTGAAACCAG CAGATCCTGC AAAAGATACG CCTCAGAGCA CCACCATTCC TTCAACCAAA 
CTGGGACCTG CTAAGGTGGA TCCGGCTGCT GGGAAGCCCT CTGGCTCAGC TGGTGCCCAA 
AAACAGACAA GTGCTCAAAA GGTGCAGGTT GAAGTGGGTC CTTCAGCAGC AAAAGTCAGT 
GCAGATGCTG ACGATCCGTT TGATGCCCTG TCTGGCACAT TACCATCATC ACAACCTTTG 
GCTCCTAAAG TCCACTACAC TGGACCTGAG ATCAAAGAGG AAAAGAAGCA TGGAGCTGGA 
GTTCCAGCTG GAGTTCCGGA GAAGCCTAAA GAAGTTCCCA AGCCCATAAG CACAGATGAT 
GCACTGGAAT CTCTCTCCTC TACGTTTGTG TCTCCAGCTC AAGCTGCTCC AAAGAAGACA 
GACGTGAAAA CTGAAACAAT AGGAGCAGTG GATGTTAAAT CGGCAGGCAC CACTAACTTT 
GCTCCCCCTC CACCATCTCA GCAGAAACAA CCGGCGTCGT CTCAAGCTGC TGCTGTAACC 
AAATCACCTG CTCCACCGGC AGACAAGAAA GCTAAAATGG AGACTCATGC ACAACCAGCC 
AAAGCAAAGA CAGAGTCGGA CAGCTCCATA TCGCTGGACG CTCTCGATGC TTTGAGCGAC 
ACACTGGGGG CTTCTAAACC ACCCCAAAAA TTACCAGAAC TCAAACCTGG ACAGATAGTT 
GATGAGAAGA AACAGACCTC AGAGAAAGGT GTTCGTGTTG GAGAAAGAGA CGACACACTT 
CCACCACAAT ACAGATTCAA AGAGGAAGAT CTCAAGAAAT ATCCTCCACC TGCGAAAGAG 
CCTTCACTGA ACACAGATGA TGCGATGGAC ATACTTTCAG GAGATTTCAC GACCCCTGCA 
TCGGCACCTG TTGTCAAAGC TCCTGTTCCT CCTGCACAGG ACACAAAGAA GCATGCTGCA 
GTGCCTGAGA AGACCAAAGA TATTCCCAAG ACTAAAGCTG ATGCTCTGTC AAGTGATTTC 
GTGGCTCCGG CACATTCTGC TCCTAAGGTT TCTTCAGCTG CTCCTAAAAC CATCCCTCCA 
GGCCCACCAC AAACCCCAGA TGAGGATCCT TTCAGTGCTT TGGGCGACAC TCTTTCTGCC 
CCAGAACCAC CAAAAAAACA ACCTGACCTG AAACCTAAGG ACATCGTACA TGAGAAGGAT 
GTGACGTCAA AGAAAGGAGT TCGTGTTGGA GAAAGAGACG ACACACTCCC ACCAGAATAC 
AGATTCAAAG AGGAAGATCT CAAGAAATAT CCTCCTCCTC AGAAAGATCC TTCCTTAGAT 
GCTAATGAAG CTCTGGATAT TCTGTCTGGG GATTTTACAA CTCCTTCCGC ACCATCGGCT 
GTTTCCCCTG CCTCTAAATC CTCTGCCAAG TCTTCAGATT CAGCTTTAGA TGCTCTTGCT 
GGTGATTTCG TCGCTTCTTC TTCTGCGTCT TCAGTTCATT CTGCCGTCTC TGGCCCCCCA 
CGCTCCGACA GACTGTTGTC AGACGGAACC TCATCAGCTC TAGATGCCCT GTCAGAAACT 
CTGGGAGACA TTAAAGCAGC CCCTGAACCC GCCCCTGTCC TACCTAAAAC TGTCGTTAAG 
GAGAAGACGG TCATAGAAGA GAGAGTGAGT AAACCGGGTG AGAGAGACGA CTCCCTTCCA 
CCAGATCAGC GGTTCACTGC GGAGGATCGC AAGGCATTTG AAGAAGCAAA GAAGAAAGAC 
GCCAAACCAA AACAGCCATC AATGGACGAT TCAGCGGCTC TGGACATGTT GTCTAGTGAT 
TTTTCAGATG TACCGACTGT AAAGCCCACT GTATCTGATG GCAAAAGCTT CACCCCTGAA 
CCAACACCAC CAACCTTTAA GGCAACCGGT CCGGTTTTAG ACAAACTGGC AGACAACGTG 
CTTTCCAACC TGACTGACCC CAAAGCCAAA GACAGCAAAG CAAAGGGAAA GGGGGCGAAA 
CCAAAGTCTA AACCAAAGAA ACAGGCAGTG GAAGACTCTT CAGCCACAGA TCTGCTGTCC 
AGTAAACCGA GCTCAGATGT GGTGCCTTCA GCTTCCACAA AGGCTGGAAA AAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324545.1
CDS	69..2525
Translation	MGQIFSWIRGTRDQPALQDIAVEQQVSLTGYYPIESQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQVTDSKSTPASSAKVSAVSSGTGVAGTTGGAGVKPADPAKDTPQSTTIPSTKLGPAKVDPAAGKPSGSAGAQKQTSAQKVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_021468870.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468870.1

ORF Insert Sequence:

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGGTCTC GCTTACTGGA TATTATCCTA TCGAGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGAA AGGAGCTACA 
CAATCGTCTA CAGCAGCTGC GACTGTCAAA TCCGGCGCTG GAGATCCTGC AACCACCCAG 
AAAGGATCTT CTCAAGTCAA ACCTCAGGTC ACAGATTCTA AATCAACACC TGCATCTTCT 
GCTAAAGTCT CAGCTGTGAG CTCTGGGACT GGAGTTGCTG GTACAACCGG TGGAGCAGGA 
GTGAAACCAG CAGATCCTGC AAAAGATACG CCTCAGAGCA CCACCATTCC TTCAACCAAA 
CTGGGACCTG CTAAGGTGGA TCCGGCTGCT GGGAAGCCCT CTGGCTCAGC TGGTGCCCAA 
AAACAGACAA GTGCTCAAAA GGTGCAGGTT GAAGTGGGTC CTTCAGCAGC AAAAGTCAGT 
GCAGATGCTG ACGATCCGTT TGATGCCCTG TCTGGCACAT TACCATCATC ACAACCTTTG 
GCTCCTAAAG TCCACTACAC TGGACCTGAG ATCAAAGAGG AAAAGAAGCA TGGAGCTGGA 
GTTCCAGCTG GAGTTCCGGA GAAGCCTAAA GAAGTTCCCA AGCCCATAAG CACAGATGAT 
GCACTGGAAT CTCTCTCCTC TACGTTTGTG TCTCCAGCTC AAGCTGCTCC AAAGAAGACA 
GACGTGAAAA CTGAAACAAT AGGAGCAGTG GATGTTAAAT CGGCAGGCAC CACTAACTTT 
GCTCCCCCTC CACCATCTCA GCAGAAACAA CCGGCGTCGT CTCAAGCTGC TGCTGTAACC 
AAATCACCTG CTCCACCGGC AGACAAGAAA GCTAAAATGG AGACTCATGC ACAACCAGCC 
AAAGCAAAGA CAGAGTCGGA CAGCTCCATA TCGCTGGACG CTCTCGATGC TTTGAGCGAC 
ACACTGGGGG CTTCTAAACC ACCCCAAAAA TTACCAGAAC TCAAACCTGG ACAGATAGTT 
GATGAGAAGA AACAGACCTC AGAGAAAGGT GTTCGTGTTG GAGAAAGAGA CGACACACTT 
CCACCACAAT ACAGATTCAA AGAGGAAGAT CTCAAGAAAT ATCCTCCACC TGCGAAAGAG 
CCTTCACTGA ACACAGATGA TGCGATGGAC ATACTTTCAG GAGATTTCAC GACCCCTGCA 
TCGGCACCTG TTGTCAAAGC TCCTGTTCCT CCTGCACAGG ACACAAAGAA GCATGCTGCA 
GTGCCTGAGA AGACCAAAGA TATTCCCAAG ACTAAAGCTG ATGCTCTGTC AAGTGATTTC 
GTGGCTCCGG CACATTCTGC TCCTAAGGTT TCTTCAGCTG CTCCTAAAAC CATCCCTCCA 
GGCCCACCAC AAACCCCAGA TGAGGATCCT TTCAGTGCTT TGGGCGACAC TCTTTCTGCC 
CCAGAACCAC CAAAAAAACA ACCTGACCTG AAACCTAAGG ACATCGTACA TGAGAAGGAT 
GTGACGTCAA AGAAAGGAGT TCGTGTTGGA GAAAGAGACG ACACACTCCC ACCAGAATAC 
AGATTCAAAG AGGAAGATCT CAAGAAATAT CCTCCTCCTC AGAAAGATCC TTCCTTAGAT 
GCTAATGAAG CTCTGGATAT TCTGTCTGGG GATTTTACAA CTCCTTCCGC ACCATCGGCT 
GTTTCCCCTG CCTCTAAATC CTCTGCCAAG TCTTCAGATT CAGCTTTAGA TGCTCTTGCT 
GGTGATTTCG TCGCTTCTTC TTCTGCGTCT TCAGTTCATT CTGCCGTCTC TGGCCCCCCA 
CGCTCCGACA GACTGTTGTC AGACGGAACC TCATCAGCTC TAGATGCCCT GTCAGAAACT 
CTGGGAGACA TTAAAGCAGC CCCTGAACCC GCCCCTGTCC TACCTAAAAC TGTCGTTAAG 
GAGAAGACGG TCATAGAAGA GAGAGTGAGT AAACCGGGTG AGAGAGACGA CTCCCTTCCA 
CCAGATCAGC GGTTCACTGC GGAGGATCGC AAGGCATTTG AAGAAGCAAA GAAGAAAGAC 
GCCAAACCAA AACAGCCATC AATGGACGAT TCAGCGGCTC TGGACATGTT GTCTAGTGAT 
TTTTCAGATG TACCGACTGT AAAGCCCACT GTATCTGATG GCAAAAGCTT CACCCCTGAA 
CCAACACCAC CAACCTTTAA GGCAACCGGT CCGGTTTTAG ACAAACTGGC AGACAACGTG 
CTTTCCAACC TGACTGACCC CAAAGCCAAA GACAGCAAAG CAAAGGGAAA GGGGGCGAAA 
CCAAAGTCTA AACCAAAGAA ACAGGCAGTG GAAGACTCTT CAGCCACAGA TCTGCTGTCC 
AGTAAACCGA GCTCAGATGT GGTGCCTTCA GCTTCCACAA AGGCTGGAAA AAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_021468871.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324546.1 Danio rerio calpastatin (cast), transcript variant X11, mRNA.

Price & Availability↑

CloneID	ODa150923
Clone ID Related Accession (Same CDS sequence)	XM_021468871.1
Accession Version	XM_021468871.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2445bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calpastatin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGGTCTC GCTTACTGGA TATTATCCTA TCGAGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGAA AGGAGCTACA 
CAATCGTCTA CAGCAGCTGC GACTGTCAAA TCCGGCGCTG GAGATCCTGC AACCACCCAG 
AAAGGATCTT CTCAAGTCAA ACCTCAGGTC ACAGATTCTA AATCAACACC TGCATCTTCT 
GCTAAAGTCT CAGCTGTGAG CTCTGGGACT GGAGTTGCTG GTACAACCGG TGGAGCAGGA 
GTGAAACCAG CAGATCCTGC AAAAGATACG CCTCAGAGCA CCACCATTCC TTCAACCAAA 
CTGGGACCTG CTAAGGTGGA TCCGGCTGCT GGGAAGCCCT CTGGCTCAGC TGGTGCCCAA 
AAACAGACAA GTGCTCAAAA GGTGCAGGTT GAAGTGGGTC CTTCAGCAGC AAAAGCTGAC 
GATCCGTTTG ATGCCCTGTC TGGCACATTA CCATCATCAC AACCTTTGGC TCCTAAAGTC 
CACTACACTG GACCTGAGAT CAAAGAGGAA AAGAAGCATG GAGCTGGAGT TCCAGCTGGA 
GTTCCGGAGA AGCCTAAAGA AGTTCCCAAG CCCATAAGCA CAGATGATGC ACTGGAATCT 
CTCTCCTCTA CGTTTGTGTC TCCAGCTCAA GCTGCTCCAA AGAAGACAGA CGTGAAAACT 
GAAACAATAG GAGCAGTGGA TGTTAAATCG GCAGGCACCA CTAACTTTGC TCCCCCTCCA 
CCATCTCAGC AGAAACAACC GGCGTCGTCT CAAGCTGCTG CTGTAACCAA ATCACCTGCT 
CCACCGGCAG ACAAGAAAGC TAAAATGGAG ACTCATGCAC AACCAGCCAA AGCAAAGACA 
GAGTCGGACA GCTCCATATC GCTGGACGCT CTCGATGCTT TGAGCGACAC ACTGGGGGCT 
TCTAAACCAC CCCAAAAATT ACCAGAACTC AAACCTGGAC AGATAGTTGA TGAGAAGAAA 
CAGACCTCAG AGAAAGGTGT TCGTGTTGGA GAAAGAGACG ACACACTTCC ACCACAATAC 
AGATTCAAAG AGGAAGATCT CAAGAAATAT CCTCCACCTG CGAAAGAGCC TTCACTGAAC 
ACAGATGATG CGATGGACAT ACTTTCAGGA GATTTCACGA CCCCTGCATC GGCACCTGTT 
GTCAAAGCTC CTGTTCCTCC TGCACAGGAC ACAAAGAAGC ATGCTGCAGT GCCTGAGAAG 
ACCAAAGATA TTCCCAAGAC TAAAGCTGAT GCTCTGTCAA GTGATTTCGT GGCTCCGGCA 
CATTCTGCTC CTAAGGTTTC TTCAGCTGCT CCTAAAACCA TCCCTCCAGG CCCACCACAA 
ACCCCAGATG AGGATCCTTT CAGTGCTTTG GGCGACACTC TTTCTGCCCC AGAACCACCA 
AAAAAACAAC CTGACCTGAA ACCTAAGGAC ATCGTACATG AGAAGGATGT GACGTCAAAG 
AAAGGAGTTC GTGTTGGAGA AAGAGACGAC ACACTCCCAC CAGAATACAG ATTCAAAGAG 
GAAGATCTCA AGAAATATCC TCCTCCTCAG AAAGATCCTT CCTTAGATGC TAATGAAGCT 
CTGGATATTC TGTCTGGGGA TTTTACAACT CCTTCCGCAC CATCGGCTGT TTCCCCTGCC 
TCTAAATCCT CTGCCAAGTC TTCAGATTCA GCTTTAGATG CTCTTGCTGG TGATTTCGTC 
GCTTCTTCTT CTGCGTCTTC AGTTCATTCT GCCGTCTCTG GCCCCCCACG CTCCGACAGA 
CTGTTGTCAG ACGGAACCTC ATCAGCTCTA GATGCCCTGT CAGAAACTCT GGGAGACATT 
AAAGCAGCCC CTGAACCCGC CCCTGTCCTA CCTAAAACTG TCGTTAAGGA GAAGACGGTC 
ATAGAAGAGA GAGTGAGTAA ACCGGGTGAG AGAGACGACT CCCTTCCACC AGATCAGCGG 
TTCACTGCGG AGGATCGCAA GGCATTTGAA GAAGCAAAGA AGAAAGACGC CAAACCAAAA 
CAGCCATCAA TGGACGATTC AGCGGCTCTG GACATGTTGT CTAGTGATTT TTCAGATGTA 
CCGACTGTAA AGCCCACTGT ATCTGATGGC AAAAGCTTCA CCCCTGAACC AACACCACCA 
ACCTTTAAGG CAACCGGTCC GGTTTTAGAC AAACTGGCAG ACAACGTGCT TTCCAACCTG 
ACTGACCCCA AAGCCAAAGA CAGCAAAGCA AAGGGAAAGG GGGCGAAACC AAAGTCTAAA 
CCAAAGAAAC AGGCAGTGGA AGACTCTTCA GCCACAGATC TGCTGTCCAG TAAACCGAGC 
TCAGATGTGG TGCCTTCAGC TTCCACAAAG GCTGGAAAAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324546.1
CDS	69..2513
Translation	MGQIFSWIRGTRDQPALQDIAVEQQVSLTGYYPIESQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQVTDSKSTPASSAKVSAVSSGTGVAGTTGGAGVKPADPAKDTPQSTTIPSTKLGPAKVDPAAGKPSGSAGAQKQTSAQKVQVEVGPSAAKADDPFDALSGTLPSSQPLAPKVHYTGPEIKEEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_021468871.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468871.1

ORF Insert Sequence:

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGGTCTC GCTTACTGGA TATTATCCTA TCGAGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGAA AGGAGCTACA 
CAATCGTCTA CAGCAGCTGC GACTGTCAAA TCCGGCGCTG GAGATCCTGC AACCACCCAG 
AAAGGATCTT CTCAAGTCAA ACCTCAGGTC ACAGATTCTA AATCAACACC TGCATCTTCT 
GCTAAAGTCT CAGCTGTGAG CTCTGGGACT GGAGTTGCTG GTACAACCGG TGGAGCAGGA 
GTGAAACCAG CAGATCCTGC AAAAGATACG CCTCAGAGCA CCACCATTCC TTCAACCAAA 
CTGGGACCTG CTAAGGTGGA TCCGGCTGCT GGGAAGCCCT CTGGCTCAGC TGGTGCCCAA 
AAACAGACAA GTGCTCAAAA GGTGCAGGTT GAAGTGGGTC CTTCAGCAGC AAAAGCTGAC 
GATCCGTTTG ATGCCCTGTC TGGCACATTA CCATCATCAC AACCTTTGGC TCCTAAAGTC 
CACTACACTG GACCTGAGAT CAAAGAGGAA AAGAAGCATG GAGCTGGAGT TCCAGCTGGA 
GTTCCGGAGA AGCCTAAAGA AGTTCCCAAG CCCATAAGCA CAGATGATGC ACTGGAATCT 
CTCTCCTCTA CGTTTGTGTC TCCAGCTCAA GCTGCTCCAA AGAAGACAGA CGTGAAAACT 
GAAACAATAG GAGCAGTGGA TGTTAAATCG GCAGGCACCA CTAACTTTGC TCCCCCTCCA 
CCATCTCAGC AGAAACAACC GGCGTCGTCT CAAGCTGCTG CTGTAACCAA ATCACCTGCT 
CCACCGGCAG ACAAGAAAGC TAAAATGGAG ACTCATGCAC AACCAGCCAA AGCAAAGACA 
GAGTCGGACA GCTCCATATC GCTGGACGCT CTCGATGCTT TGAGCGACAC ACTGGGGGCT 
TCTAAACCAC CCCAAAAATT ACCAGAACTC AAACCTGGAC AGATAGTTGA TGAGAAGAAA 
CAGACCTCAG AGAAAGGTGT TCGTGTTGGA GAAAGAGACG ACACACTTCC ACCACAATAC 
AGATTCAAAG AGGAAGATCT CAAGAAATAT CCTCCACCTG CGAAAGAGCC TTCACTGAAC 
ACAGATGATG CGATGGACAT ACTTTCAGGA GATTTCACGA CCCCTGCATC GGCACCTGTT 
GTCAAAGCTC CTGTTCCTCC TGCACAGGAC ACAAAGAAGC ATGCTGCAGT GCCTGAGAAG 
ACCAAAGATA TTCCCAAGAC TAAAGCTGAT GCTCTGTCAA GTGATTTCGT GGCTCCGGCA 
CATTCTGCTC CTAAGGTTTC TTCAGCTGCT CCTAAAACCA TCCCTCCAGG CCCACCACAA 
ACCCCAGATG AGGATCCTTT CAGTGCTTTG GGCGACACTC TTTCTGCCCC AGAACCACCA 
AAAAAACAAC CTGACCTGAA ACCTAAGGAC ATCGTACATG AGAAGGATGT GACGTCAAAG 
AAAGGAGTTC GTGTTGGAGA AAGAGACGAC ACACTCCCAC CAGAATACAG ATTCAAAGAG 
GAAGATCTCA AGAAATATCC TCCTCCTCAG AAAGATCCTT CCTTAGATGC TAATGAAGCT 
CTGGATATTC TGTCTGGGGA TTTTACAACT CCTTCCGCAC CATCGGCTGT TTCCCCTGCC 
TCTAAATCCT CTGCCAAGTC TTCAGATTCA GCTTTAGATG CTCTTGCTGG TGATTTCGTC 
GCTTCTTCTT CTGCGTCTTC AGTTCATTCT GCCGTCTCTG GCCCCCCACG CTCCGACAGA 
CTGTTGTCAG ACGGAACCTC ATCAGCTCTA GATGCCCTGT CAGAAACTCT GGGAGACATT 
AAAGCAGCCC CTGAACCCGC CCCTGTCCTA CCTAAAACTG TCGTTAAGGA GAAGACGGTC 
ATAGAAGAGA GAGTGAGTAA ACCGGGTGAG AGAGACGACT CCCTTCCACC AGATCAGCGG 
TTCACTGCGG AGGATCGCAA GGCATTTGAA GAAGCAAAGA AGAAAGACGC CAAACCAAAA 
CAGCCATCAA TGGACGATTC AGCGGCTCTG GACATGTTGT CTAGTGATTT TTCAGATGTA 
CCGACTGTAA AGCCCACTGT ATCTGATGGC AAAAGCTTCA CCCCTGAACC AACACCACCA 
ACCTTTAAGG CAACCGGTCC GGTTTTAGAC AAACTGGCAG ACAACGTGCT TTCCAACCTG 
ACTGACCCCA AAGCCAAAGA CAGCAAAGCA AAGGGAAAGG GGGCGAAACC AAAGTCTAAA 
CCAAAGAAAC AGGCAGTGGA AGACTCTTCA GCCACAGATC TGCTGTCCAG TAAACCGAGC 
TCAGATGTGG TGCCTTCAGC TTCCACAAAG GCTGGAAAAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_021468872.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324547.1 Danio rerio calpastatin (cast), transcript variant X14, mRNA.

Price & Availability↑

CloneID	ODa150924
Clone ID Related Accession (Same CDS sequence)	XM_021468872.1
Accession Version	XM_021468872.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calpastatin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGGTCTC GCTTACTGGA TATTATCCTA TCGAGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGAA AGGAGCTACA 
CAATCGTCTA CAGCAGCTGC GACTGTCAAA TCCGGCGCTG GAGATCCTGC AACCACCCAG 
AAAGGATCTT CTCAAGTCAA ACCTCAGAGC ACCACCATTC CTTCAACCAA ACTGGGACCT 
GCTAAGGTGG ATCCGGCTGC TGGGAAGCCC TCTGGCTCAG CTGGTGCCCA AAAACAGACA 
AGTGCTCAAA AGGTGCAGGT TGAAGTGGGT CCTTCAGCAG CAAAAGTCAG TGCAGATGCT 
GACGATCCGT TTGATGCCCT GTCTGGCACA TTACCATCAT CACAACCTTT GGCTCCTAAA 
GTCCACTACA CTGGACCTGA GATCAAAGAG CCCAAACTGA AATCAGAGAA AGGTGTTTTG 
TGTGGTGAGC GAGATGACAC GCTGCCGCCC GGATACAGAA AGGAAGACCT GGAAAAGAAG 
CATGGAGCTG GAGTTCCAGC TGGAGTTCCG GAGAAGCCTA AAGAAGTTCC CAAGCCCATA 
AGCACAGATG ATGCACTGGA ATCTCTCTCC TCTACGTTTG TGTCTCCAGC TCAAGCTGCT 
CCAAAGAAGA CAGACGTGAA AACTGAAACA ATAGGAGCAG TGGATGTTAA ATCGGCAGGC 
ACCACTAACT TTGCTCCCCC TCCACCATCT CAGCAGAAAC AACCGGCGTC GTCTCAAGCT 
GCTGCTGTAA CCAAATCACC TGCTCCACCG GCAGACAAGA AAGCTAAAAT GGAGACTCAT 
GCACAACCAG CCAAAGCAAA GACAGAGTCG GACAGCTCCA TATCGCTGGA CGCTCTCGAT 
GCTTTGAGCG ACACACTGGG GGCTTCTAAA CCACCCCAAA AATTACCAGA ACTCAAACCT 
GGACAGATAG TTGATGAGAA GAAACAGACC TCAGAGAAAG GTGTTCGTGT TGGAGAAAGA 
GACGACACAC TTCCACCACA ATACAGATTC AAAGAGGAAG ATCTCAAGAA ATATCCTCCA 
CCTGCGAAAG AGCCTTCACT GAACACAGAT GATGCGATGG ACATACTTTC AGGAGATTTC 
ACGACCCCTG CATCGGCACC TGTTGTCAAA GCTCCTGTTC CTCCTGCACA GGACACAAAG 
AAGCATGCTG CAGTGCCTGA GAAGACCAAA GATATTCCCA AGACTAAAGC TGATGCTCTG 
TCAAGTGATT TCGTGGCTCC GGCACATTCT GCTCCTAAGG TTTCTTCAGC TGCTCCTAAA 
ACCATCCCTC CAGGCCCACC ACAAACCCCA GATGAGGATC CTTTCAGTGC TTTGGGCGAC 
ACTCTTTCTG CCCCAGAACC ACCAAAAAAA CAACCTGACC TGAAACCTAA GGACATCGTA 
CATGAGAAGG ATGTGACGTC AAAGAAAGGA GTTCGTGTTG GAGAAAGAGA CGACACACTC 
CCACCAGAAT ACAGATTCAA AGAGGAAGAT CTCAAGAAAT ATCCTCCTCC TCAGAAAGAT 
CCTTCCTTAG ATGCTAATGA AGCTCTGGAT ATTCTGTCTG GGGATTTTAC AACTCCTTCC 
GCACCATCGG CTGTTTCCCC TGCCTCTAAA TCCTCTGCCA AGTCTTCAGA TTCAGCTTTA 
GATGCTCTTG CTGGTGATTT CGTCGCTTCT TCTTCTGCGT CTTCAGTTCA TTCTGCCGTC 
TCTGGCCCCC CACGCTCCGA CAGACTGTTG TCAGACGGAA CCTCATCAGC TCTAGATGCC 
CTGTCAGAAA CTCTGGGAGA CATTAAAGCA GCCCCTGAAC CCGCCCCTGT CCTACCTAAA 
ACTGTCGTTA AGGAGAAGAC GGTCATAGAA GAGAGAGTGA GTAAACCGGG TGAGAGAGAC 
GACTCCCTTC CACCAGATCA GCGGTTCACT GCGGAGGATC GCAAGGCATT TGAAGAAGCA 
AAGAAGAAAG ACGCCAAACC AAAACAGCCA TCAATGGACG ATTCAGCGGC TCTGGACATG 
TTGTCTAGTG ATTTTTCAGA TGTACCGACT GTAAAGCCCA CTGTATCTGA TGGCAAAAGC 
TTCACCCCTG AACCAACACC ACCAACCTTT AAGGCAACCG GTCCGGTTTT AGACAAACTG 
GCAGACAACG TGCTTTCCAA CCTGACTGAC CCCAAAGCCA AAGACAGCAA AGCAAAGGGA 
AAGGGGGCGA AACCAAAGTC TAAACCAAAG AAACAGGCAG TGGAAGACTC TTCAGCCACA 
GATCTGCTGT CCAGTAAACC GAGCTCAGAT GTGGTGCCTT CAGCTTCCAC AAAGGCTGGA 
AAAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324547.1
CDS	69..2477
Translation	MGQIFSWIRGTRDQPALQDIAVEQQVSLTGYYPIESQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQSTTIPSTKLGPAKVDPAAGKPSGSAGAQKQTSAQKVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_021468872.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468872.1

ORF Insert Sequence:

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGGTCTC GCTTACTGGA TATTATCCTA TCGAGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGAA AGGAGCTACA 
CAATCGTCTA CAGCAGCTGC GACTGTCAAA TCCGGCGCTG GAGATCCTGC AACCACCCAG 
AAAGGATCTT CTCAAGTCAA ACCTCAGAGC ACCACCATTC CTTCAACCAA ACTGGGACCT 
GCTAAGGTGG ATCCGGCTGC TGGGAAGCCC TCTGGCTCAG CTGGTGCCCA AAAACAGACA 
AGTGCTCAAA AGGTGCAGGT TGAAGTGGGT CCTTCAGCAG CAAAAGTCAG TGCAGATGCT 
GACGATCCGT TTGATGCCCT GTCTGGCACA TTACCATCAT CACAACCTTT GGCTCCTAAA 
GTCCACTACA CTGGACCTGA GATCAAAGAG CCCAAACTGA AATCAGAGAA AGGTGTTTTG 
TGTGGTGAGC GAGATGACAC GCTGCCGCCC GGATACAGAA AGGAAGACCT GGAAAAGAAG 
CATGGAGCTG GAGTTCCAGC TGGAGTTCCG GAGAAGCCTA AAGAAGTTCC CAAGCCCATA 
AGCACAGATG ATGCACTGGA ATCTCTCTCC TCTACGTTTG TGTCTCCAGC TCAAGCTGCT 
CCAAAGAAGA CAGACGTGAA AACTGAAACA ATAGGAGCAG TGGATGTTAA ATCGGCAGGC 
ACCACTAACT TTGCTCCCCC TCCACCATCT CAGCAGAAAC AACCGGCGTC GTCTCAAGCT 
GCTGCTGTAA CCAAATCACC TGCTCCACCG GCAGACAAGA AAGCTAAAAT GGAGACTCAT 
GCACAACCAG CCAAAGCAAA GACAGAGTCG GACAGCTCCA TATCGCTGGA CGCTCTCGAT 
GCTTTGAGCG ACACACTGGG GGCTTCTAAA CCACCCCAAA AATTACCAGA ACTCAAACCT 
GGACAGATAG TTGATGAGAA GAAACAGACC TCAGAGAAAG GTGTTCGTGT TGGAGAAAGA 
GACGACACAC TTCCACCACA ATACAGATTC AAAGAGGAAG ATCTCAAGAA ATATCCTCCA 
CCTGCGAAAG AGCCTTCACT GAACACAGAT GATGCGATGG ACATACTTTC AGGAGATTTC 
ACGACCCCTG CATCGGCACC TGTTGTCAAA GCTCCTGTTC CTCCTGCACA GGACACAAAG 
AAGCATGCTG CAGTGCCTGA GAAGACCAAA GATATTCCCA AGACTAAAGC TGATGCTCTG 
TCAAGTGATT TCGTGGCTCC GGCACATTCT GCTCCTAAGG TTTCTTCAGC TGCTCCTAAA 
ACCATCCCTC CAGGCCCACC ACAAACCCCA GATGAGGATC CTTTCAGTGC TTTGGGCGAC 
ACTCTTTCTG CCCCAGAACC ACCAAAAAAA CAACCTGACC TGAAACCTAA GGACATCGTA 
CATGAGAAGG ATGTGACGTC AAAGAAAGGA GTTCGTGTTG GAGAAAGAGA CGACACACTC 
CCACCAGAAT ACAGATTCAA AGAGGAAGAT CTCAAGAAAT ATCCTCCTCC TCAGAAAGAT 
CCTTCCTTAG ATGCTAATGA AGCTCTGGAT ATTCTGTCTG GGGATTTTAC AACTCCTTCC 
GCACCATCGG CTGTTTCCCC TGCCTCTAAA TCCTCTGCCA AGTCTTCAGA TTCAGCTTTA 
GATGCTCTTG CTGGTGATTT CGTCGCTTCT TCTTCTGCGT CTTCAGTTCA TTCTGCCGTC 
TCTGGCCCCC CACGCTCCGA CAGACTGTTG TCAGACGGAA CCTCATCAGC TCTAGATGCC 
CTGTCAGAAA CTCTGGGAGA CATTAAAGCA GCCCCTGAAC CCGCCCCTGT CCTACCTAAA 
ACTGTCGTTA AGGAGAAGAC GGTCATAGAA GAGAGAGTGA GTAAACCGGG TGAGAGAGAC 
GACTCCCTTC CACCAGATCA GCGGTTCACT GCGGAGGATC GCAAGGCATT TGAAGAAGCA 
AAGAAGAAAG ACGCCAAACC AAAACAGCCA TCAATGGACG ATTCAGCGGC TCTGGACATG 
TTGTCTAGTG ATTTTTCAGA TGTACCGACT GTAAAGCCCA CTGTATCTGA TGGCAAAAGC 
TTCACCCCTG AACCAACACC ACCAACCTTT AAGGCAACCG GTCCGGTTTT AGACAAACTG 
GCAGACAACG TGCTTTCCAA CCTGACTGAC CCCAAAGCCA AAGACAGCAA AGCAAAGGGA 
AAGGGGGCGA AACCAAAGTC TAAACCAAAG AAACAGGCAG TGGAAGACTC TTCAGCCACA 
GATCTGCTGT CCAGTAAACC GAGCTCAGAT GTGGTGCCTT CAGCTTCCAC AAAGGCTGGA 
AAAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_021468874.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324549.1 Danio rerio calpastatin (cast), transcript variant X17, mRNA.

Price & Availability↑

CloneID	ODa150925
Clone ID Related Accession (Same CDS sequence)	XM_021468874.1
Accession Version	XM_021468874.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calpastatin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGGTCTC GCTTACTGGA TATTATCCTA TCGAGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGAA AGGAGCTACA 
CAATCGTCTA CAGCAGCTGC GACTGTCAAA TCCGGCGCTG GAGATCCTGC AACCACCCAG 
AAAGGATCTT CTCAAGTCAA ACCTCAGGTG CAGGTTGAAG TGGGTCCTTC AGCAGCAAAA 
GTCAGTGCAG ATGCTGACGA TCCGTTTGAT GCCCTGTCTG GCACATTACC ATCATCACAA 
CCTTTGGCTC CTAAAGTCCA CTACACTGGA CCTGAGATCA AAGAGCCCAA ACTGAAATCA 
GAGAAAGGTG TTTTGTGTGG TGAGCGAGAT GACACGCTGC CGCCCGGATA CAGAAAGGAA 
GACCTGGAAA AGAAGCATGG AGCTGGAGTT CCAGCTGGAG TTCCGGAGAA GCCTAAAGAA 
GTTCCCAAGC CCATAAGCAC AGATGATGCA CTGGAATCTC TCTCCTCTAC GTTTGTGTCT 
CCAGCTCAAG CTGCTCCAAA GAAGACAGAC GTGAAAACTG AAACAATAGG AGCAGTGGAT 
GTTAAATCGG CAGGCACCAC TAACTTTGCT CCCCCTCCAC CATCTCAGCA GAAACAACCG 
GCGTCGTCTC AAGCTGCTGC TGTAACCAAA TCACCTGCTC CACCGGCAGA CAAGAAAGCT 
AAAATGGAGA CTCATGCACA ACCAGCCAAA GCAAAGACAG AGTCGGACAG CTCCATATCG 
CTGGACGCTC TCGATGCTTT GAGCGACACA CTGGGGGCTT CTAAACCACC CCAAAAATTA 
CCAGAACTCA AACCTGGACA GATAGTTGAT GAGAAGAAAC AGACCTCAGA GAAAGGTGTT 
CGTGTTGGAG AAAGAGACGA CACACTTCCA CCACAATACA GATTCAAAGA GGAAGATCTC 
AAGAAATATC CTCCACCTGC GAAAGAGCCT TCACTGAACA CAGATGATGC GATGGACATA 
CTTTCAGGAG ATTTCACGAC CCCTGCATCG GCACCTGTTG TCAAAGCTCC TGTTCCTCCT 
GCACAGGACA CAAAGAAGCA TGCTGCAGTG CCTGAGAAGA CCAAAGATAT TCCCAAGACT 
AAAGCTGATG CTCTGTCAAG TGATTTCGTG GCTCCGGCAC ATTCTGCTCC TAAGGTTTCT 
TCAGCTGCTC CTAAAACCAT CCCTCCAGGC CCACCACAAA CCCCAGATGA GGATCCTTTC 
AGTGCTTTGG GCGACACTCT TTCTGCCCCA GAACCACCAA AAAAACAACC TGACCTGAAA 
CCTAAGGACA TCGTACATGA GAAGGATGTG ACGTCAAAGA AAGGAGTTCG TGTTGGAGAA 
AGAGACGACA CACTCCCACC AGAATACAGA TTCAAAGAGG AAGATCTCAA GAAATATCCT 
CCTCCTCAGA AAGATCCTTC CTTAGATGCT AATGAAGCTC TGGATATTCT GTCTGGGGAT 
TTTACAACTC CTTCCGCACC ATCGGCTGTT TCCCCTGCCT CTAAATCCTC TGCCAAGTCT 
TCAGATTCAG CTTTAGATGC TCTTGCTGGT GATTTCGTCG CTTCTTCTTC TGCGTCTTCA 
GTTCATTCTG CCGTCTCTGG CCCCCCACGC TCCGACAGAC TGTTGTCAGA CGGAACCTCA 
TCAGCTCTAG ATGCCCTGTC AGAAACTCTG GGAGACATTA AAGCAGCCCC TGAACCCGCC 
CCTGTCCTAC CTAAAACTGT CGTTAAGGAG AAGACGGTCA TAGAAGAGAG AGTGAGTAAA 
CCGGGTGAGA GAGACGACTC CCTTCCACCA GATCAGCGGT TCACTGCGGA GGATCGCAAG 
GCATTTGAAG AAGCAAAGAA GAAAGACGCC AAACCAAAAC AGCCATCAAT GGACGATTCA 
GCGGCTCTGG ACATGTTGTC TAGTGATTTT TCAGATGTAC CGACTGTAAA GCCCACTGTA 
TCTGATGGCA AAAGCTTCAC CCCTGAACCA ACACCACCAA CCTTTAAGGC AACCGGTCCG 
GTTTTAGACA AACTGGCAGA CAACGTGCTT TCCAACCTGA CTGACCCCAA AGCCAAAGAC 
AGCAAAGCAA AGGGAAAGGG GGCGAAACCA AAGTCTAAAC CAAAGAAACA GGCAGTGGAA 
GACTCTTCAG CCACAGATCT GCTGTCCAGT AAACCGAGCT CAGATGTGGT GCCTTCAGCT 
TCCACAAAGG CTGGAAAAAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324549.1
CDS	69..2372
Translation	MGQIFSWIRGTRDQPALQDIAVEQQVSLTGYYPIESQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_021468874.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468874.1

ORF Insert Sequence:

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGGTCTC GCTTACTGGA TATTATCCTA TCGAGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGAA AGGAGCTACA 
CAATCGTCTA CAGCAGCTGC GACTGTCAAA TCCGGCGCTG GAGATCCTGC AACCACCCAG 
AAAGGATCTT CTCAAGTCAA ACCTCAGGTG CAGGTTGAAG TGGGTCCTTC AGCAGCAAAA 
GTCAGTGCAG ATGCTGACGA TCCGTTTGAT GCCCTGTCTG GCACATTACC ATCATCACAA 
CCTTTGGCTC CTAAAGTCCA CTACACTGGA CCTGAGATCA AAGAGCCCAA ACTGAAATCA 
GAGAAAGGTG TTTTGTGTGG TGAGCGAGAT GACACGCTGC CGCCCGGATA CAGAAAGGAA 
GACCTGGAAA AGAAGCATGG AGCTGGAGTT CCAGCTGGAG TTCCGGAGAA GCCTAAAGAA 
GTTCCCAAGC CCATAAGCAC AGATGATGCA CTGGAATCTC TCTCCTCTAC GTTTGTGTCT 
CCAGCTCAAG CTGCTCCAAA GAAGACAGAC GTGAAAACTG AAACAATAGG AGCAGTGGAT 
GTTAAATCGG CAGGCACCAC TAACTTTGCT CCCCCTCCAC CATCTCAGCA GAAACAACCG 
GCGTCGTCTC AAGCTGCTGC TGTAACCAAA TCACCTGCTC CACCGGCAGA CAAGAAAGCT 
AAAATGGAGA CTCATGCACA ACCAGCCAAA GCAAAGACAG AGTCGGACAG CTCCATATCG 
CTGGACGCTC TCGATGCTTT GAGCGACACA CTGGGGGCTT CTAAACCACC CCAAAAATTA 
CCAGAACTCA AACCTGGACA GATAGTTGAT GAGAAGAAAC AGACCTCAGA GAAAGGTGTT 
CGTGTTGGAG AAAGAGACGA CACACTTCCA CCACAATACA GATTCAAAGA GGAAGATCTC 
AAGAAATATC CTCCACCTGC GAAAGAGCCT TCACTGAACA CAGATGATGC GATGGACATA 
CTTTCAGGAG ATTTCACGAC CCCTGCATCG GCACCTGTTG TCAAAGCTCC TGTTCCTCCT 
GCACAGGACA CAAAGAAGCA TGCTGCAGTG CCTGAGAAGA CCAAAGATAT TCCCAAGACT 
AAAGCTGATG CTCTGTCAAG TGATTTCGTG GCTCCGGCAC ATTCTGCTCC TAAGGTTTCT 
TCAGCTGCTC CTAAAACCAT CCCTCCAGGC CCACCACAAA CCCCAGATGA GGATCCTTTC 
AGTGCTTTGG GCGACACTCT TTCTGCCCCA GAACCACCAA AAAAACAACC TGACCTGAAA 
CCTAAGGACA TCGTACATGA GAAGGATGTG ACGTCAAAGA AAGGAGTTCG TGTTGGAGAA 
AGAGACGACA CACTCCCACC AGAATACAGA TTCAAAGAGG AAGATCTCAA GAAATATCCT 
CCTCCTCAGA AAGATCCTTC CTTAGATGCT AATGAAGCTC TGGATATTCT GTCTGGGGAT 
TTTACAACTC CTTCCGCACC ATCGGCTGTT TCCCCTGCCT CTAAATCCTC TGCCAAGTCT 
TCAGATTCAG CTTTAGATGC TCTTGCTGGT GATTTCGTCG CTTCTTCTTC TGCGTCTTCA 
GTTCATTCTG CCGTCTCTGG CCCCCCACGC TCCGACAGAC TGTTGTCAGA CGGAACCTCA 
TCAGCTCTAG ATGCCCTGTC AGAAACTCTG GGAGACATTA AAGCAGCCCC TGAACCCGCC 
CCTGTCCTAC CTAAAACTGT CGTTAAGGAG AAGACGGTCA TAGAAGAGAG AGTGAGTAAA 
CCGGGTGAGA GAGACGACTC CCTTCCACCA GATCAGCGGT TCACTGCGGA GGATCGCAAG 
GCATTTGAAG AAGCAAAGAA GAAAGACGCC AAACCAAAAC AGCCATCAAT GGACGATTCA 
GCGGCTCTGG ACATGTTGTC TAGTGATTTT TCAGATGTAC CGACTGTAAA GCCCACTGTA 
TCTGATGGCA AAAGCTTCAC CCCTGAACCA ACACCACCAA CCTTTAAGGC AACCGGTCCG 
GTTTTAGACA AACTGGCAGA CAACGTGCTT TCCAACCTGA CTGACCCCAA AGCCAAAGAC 
AGCAAAGCAA AGGGAAAGGG GGCGAAACCA AAGTCTAAAC CAAAGAAACA GGCAGTGGAA 
GACTCTTCAG CCACAGATCT GCTGTCCAGT AAACCGAGCT CAGATGTGGT GCCTTCAGCT 
TCCACAAAGG CTGGAAAAAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_021468875.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324550.1 Danio rerio calpastatin (cast), transcript variant X18, mRNA.

Price & Availability↑

CloneID	ODa150926
Clone ID Related Accession (Same CDS sequence)	XM_021468875.1
Accession Version	XM_021468875.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calpastatin isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGGTCTC GCTTACTGGA TATTATCCTA TCGAGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGAA AGGAGCTACA 
CAATCGTCTA CAGCAGCTGC GACTGTCAAA TCCGGCGCTG GAGATCCTGC AACCACCCAG 
AAAGGATCTT CTCAAGTCAA ACCTCAGGTG CAGGTTGAAG TGGGTCCTTC AGCAGCAAAA 
GCTGACGATC CGTTTGATGC CCTGTCTGGC ACATTACCAT CATCACAACC TTTGGCTCCT 
AAAGTCCACT ACACTGGACC TGAGATCAAA GAGCCCAAAC TGAAATCAGA GAAAGGTGTT 
TTGTGTGGTG AGCGAGATGA CACGCTGCCG CCCGGATACA GAAAGGAAGA CCTGGAAAAG 
AAGCATGGAG CTGGAGTTCC AGCTGGAGTT CCGGAGAAGC CTAAAGAAGT TCCCAAGCCC 
ATAAGCACAG ATGATGCACT GGAATCTCTC TCCTCTACGT TTGTGTCTCC AGCTCAAGCT 
GCTCCAAAGA AGACAGACGT GAAAACTGAA ACAATAGGAG CAGTGGATGT TAAATCGGCA 
GGCACCACTA ACTTTGCTCC CCCTCCACCA TCTCAGCAGA AACAACCGGC GTCGTCTCAA 
GCTGCTGCTG TAACCAAATC ACCTGCTCCA CCGGCAGACA AGAAAGCTAA AATGGAGACT 
CATGCACAAC CAGCCAAAGC AAAGACAGAG TCGGACAGCT CCATATCGCT GGACGCTCTC 
GATGCTTTGA GCGACACACT GGGGGCTTCT AAACCACCCC AAAAATTACC AGAACTCAAA 
CCTGGACAGA TAGTTGATGA GAAGAAACAG ACCTCAGAGA AAGGTGTTCG TGTTGGAGAA 
AGAGACGACA CACTTCCACC ACAATACAGA TTCAAAGAGG AAGATCTCAA GAAATATCCT 
CCACCTGCGA AAGAGCCTTC ACTGAACACA GATGATGCGA TGGACATACT TTCAGGAGAT 
TTCACGACCC CTGCATCGGC ACCTGTTGTC AAAGCTCCTG TTCCTCCTGC ACAGGACACA 
AAGAAGCATG CTGCAGTGCC TGAGAAGACC AAAGATATTC CCAAGACTAA AGCTGATGCT 
CTGTCAAGTG ATTTCGTGGC TCCGGCACAT TCTGCTCCTA AGGTTTCTTC AGCTGCTCCT 
AAAACCATCC CTCCAGGCCC ACCACAAACC CCAGATGAGG ATCCTTTCAG TGCTTTGGGC 
GACACTCTTT CTGCCCCAGA ACCACCAAAA AAACAACCTG ACCTGAAACC TAAGGACATC 
GTACATGAGA AGGATGTGAC GTCAAAGAAA GGAGTTCGTG TTGGAGAAAG AGACGACACA 
CTCCCACCAG AATACAGATT CAAAGAGGAA GATCTCAAGA AATATCCTCC TCCTCAGAAA 
GATCCTTCCT TAGATGCTAA TGAAGCTCTG GATATTCTGT CTGGGGATTT TACAACTCCT 
TCCGCACCAT CGGCTGTTTC CCCTGCCTCT AAATCCTCTG CCAAGTCTTC AGATTCAGCT 
TTAGATGCTC TTGCTGGTGA TTTCGTCGCT TCTTCTTCTG CGTCTTCAGT TCATTCTGCC 
GTCTCTGGCC CCCCACGCTC CGACAGACTG TTGTCAGACG GAACCTCATC AGCTCTAGAT 
GCCCTGTCAG AAACTCTGGG AGACATTAAA GCAGCCCCTG AACCCGCCCC TGTCCTACCT 
AAAACTGTCG TTAAGGAGAA GACGGTCATA GAAGAGAGAG TGAGTAAACC GGGTGAGAGA 
GACGACTCCC TTCCACCAGA TCAGCGGTTC ACTGCGGAGG ATCGCAAGGC ATTTGAAGAA 
GCAAAGAAGA AAGACGCCAA ACCAAAACAG CCATCAATGG ACGATTCAGC GGCTCTGGAC 
ATGTTGTCTA GTGATTTTTC AGATGTACCG ACTGTAAAGC CCACTGTATC TGATGGCAAA 
AGCTTCACCC CTGAACCAAC ACCACCAACC TTTAAGGCAA CCGGTCCGGT TTTAGACAAA 
CTGGCAGACA ACGTGCTTTC CAACCTGACT GACCCCAAAG CCAAAGACAG CAAAGCAAAG 
GGAAAGGGGG CGAAACCAAA GTCTAAACCA AAGAAACAGG CAGTGGAAGA CTCTTCAGCC 
ACAGATCTGC TGTCCAGTAA ACCGAGCTCA GATGTGGTGC CTTCAGCTTC CACAAAGGCT 
GGAAAAAGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324550.1
CDS	69..2360
Translation	MGQIFSWIRGTRDQPALQDIAVEQQVSLTGYYPIESQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQVQVEVGPSAAKADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_021468875.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468875.1

ORF Insert Sequence:

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGGTCTC GCTTACTGGA TATTATCCTA TCGAGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGAA AGGAGCTACA 
CAATCGTCTA CAGCAGCTGC GACTGTCAAA TCCGGCGCTG GAGATCCTGC AACCACCCAG 
AAAGGATCTT CTCAAGTCAA ACCTCAGGTG CAGGTTGAAG TGGGTCCTTC AGCAGCAAAA 
GCTGACGATC CGTTTGATGC CCTGTCTGGC ACATTACCAT CATCACAACC TTTGGCTCCT 
AAAGTCCACT ACACTGGACC TGAGATCAAA GAGCCCAAAC TGAAATCAGA GAAAGGTGTT 
TTGTGTGGTG AGCGAGATGA CACGCTGCCG CCCGGATACA GAAAGGAAGA CCTGGAAAAG 
AAGCATGGAG CTGGAGTTCC AGCTGGAGTT CCGGAGAAGC CTAAAGAAGT TCCCAAGCCC 
ATAAGCACAG ATGATGCACT GGAATCTCTC TCCTCTACGT TTGTGTCTCC AGCTCAAGCT 
GCTCCAAAGA AGACAGACGT GAAAACTGAA ACAATAGGAG CAGTGGATGT TAAATCGGCA 
GGCACCACTA ACTTTGCTCC CCCTCCACCA TCTCAGCAGA AACAACCGGC GTCGTCTCAA 
GCTGCTGCTG TAACCAAATC ACCTGCTCCA CCGGCAGACA AGAAAGCTAA AATGGAGACT 
CATGCACAAC CAGCCAAAGC AAAGACAGAG TCGGACAGCT CCATATCGCT GGACGCTCTC 
GATGCTTTGA GCGACACACT GGGGGCTTCT AAACCACCCC AAAAATTACC AGAACTCAAA 
CCTGGACAGA TAGTTGATGA GAAGAAACAG ACCTCAGAGA AAGGTGTTCG TGTTGGAGAA 
AGAGACGACA CACTTCCACC ACAATACAGA TTCAAAGAGG AAGATCTCAA GAAATATCCT 
CCACCTGCGA AAGAGCCTTC ACTGAACACA GATGATGCGA TGGACATACT TTCAGGAGAT 
TTCACGACCC CTGCATCGGC ACCTGTTGTC AAAGCTCCTG TTCCTCCTGC ACAGGACACA 
AAGAAGCATG CTGCAGTGCC TGAGAAGACC AAAGATATTC CCAAGACTAA AGCTGATGCT 
CTGTCAAGTG ATTTCGTGGC TCCGGCACAT TCTGCTCCTA AGGTTTCTTC AGCTGCTCCT 
AAAACCATCC CTCCAGGCCC ACCACAAACC CCAGATGAGG ATCCTTTCAG TGCTTTGGGC 
GACACTCTTT CTGCCCCAGA ACCACCAAAA AAACAACCTG ACCTGAAACC TAAGGACATC 
GTACATGAGA AGGATGTGAC GTCAAAGAAA GGAGTTCGTG TTGGAGAAAG AGACGACACA 
CTCCCACCAG AATACAGATT CAAAGAGGAA GATCTCAAGA AATATCCTCC TCCTCAGAAA 
GATCCTTCCT TAGATGCTAA TGAAGCTCTG GATATTCTGT CTGGGGATTT TACAACTCCT 
TCCGCACCAT CGGCTGTTTC CCCTGCCTCT AAATCCTCTG CCAAGTCTTC AGATTCAGCT 
TTAGATGCTC TTGCTGGTGA TTTCGTCGCT TCTTCTTCTG CGTCTTCAGT TCATTCTGCC 
GTCTCTGGCC CCCCACGCTC CGACAGACTG TTGTCAGACG GAACCTCATC AGCTCTAGAT 
GCCCTGTCAG AAACTCTGGG AGACATTAAA GCAGCCCCTG AACCCGCCCC TGTCCTACCT 
AAAACTGTCG TTAAGGAGAA GACGGTCATA GAAGAGAGAG TGAGTAAACC GGGTGAGAGA 
GACGACTCCC TTCCACCAGA TCAGCGGTTC ACTGCGGAGG ATCGCAAGGC ATTTGAAGAA 
GCAAAGAAGA AAGACGCCAA ACCAAAACAG CCATCAATGG ACGATTCAGC GGCTCTGGAC 
ATGTTGTCTA GTGATTTTTC AGATGTACCG ACTGTAAAGC CCACTGTATC TGATGGCAAA 
AGCTTCACCC CTGAACCAAC ACCACCAACC TTTAAGGCAA CCGGTCCGGT TTTAGACAAA 
CTGGCAGACA ACGTGCTTTC CAACCTGACT GACCCCAAAG CCAAAGACAG CAAAGCAAAG 
GGAAAGGGGG CGAAACCAAA GTCTAAACCA AAGAAACAGG CAGTGGAAGA CTCTTCAGCC 
ACAGATCTGC TGTCCAGTAA ACCGAGCTCA GATGTGGTGC CTTCAGCTTC CACAAAGGCT 
GGAAAAAGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_021468876.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324551.1 Danio rerio calpastatin (cast), transcript variant X20, mRNA.

Price & Availability↑

CloneID	ODa150927
Clone ID Related Accession (Same CDS sequence)	XM_021468876.1
Accession Version	XM_021468876.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calpastatin isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGGTCTC GCTTACTGGA TATTATCCTA TCGAGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGGT GCAGGTTGAA 
GTGGGTCCTT CAGCAGCAAA AGTCAGTGCA GATGCTGACG ATCCGTTTGA TGCCCTGTCT 
GGCACATTAC CATCATCACA ACCTTTGGCT CCTAAAGTCC ACTACACTGG ACCTGAGATC 
AAAGAGCCCA AACTGAAATC AGAGAAAGGT GTTTTGTGTG GTGAGCGAGA TGACACGCTG 
CCGCCCGGAT ACAGAAAGGA AGACCTGGAA AAGAAGCATG GAGCTGGAGT TCCAGCTGGA 
GTTCCGGAGA AGCCTAAAGA AGTTCCCAAG CCCATAAGCA CAGATGATGC ACTGGAATCT 
CTCTCCTCTA CGTTTGTGTC TCCAGCTCAA GCTGCTCCAA AGAAGACAGA CGTGAAAACT 
GAAACAATAG GAGCAGTGGA TGTTAAATCG GCAGGCACCA CTAACTTTGC TCCCCCTCCA 
CCATCTCAGC AGAAACAACC GGCGTCGTCT CAAGCTGCTG CTGTAACCAA ATCACCTGCT 
CCACCGGCAG ACAAGAAAGC TAAAATGGAG ACTCATGCAC AACCAGCCAA AGCAAAGACA 
GAGTCGGACA GCTCCATATC GCTGGACGCT CTCGATGCTT TGAGCGACAC ACTGGGGGCT 
TCTAAACCAC CCCAAAAATT ACCAGAACTC AAACCTGGAC AGATAGTTGA TGAGAAGAAA 
CAGACCTCAG AGAAAGGTGT TCGTGTTGGA GAAAGAGACG ACACACTTCC ACCACAATAC 
AGATTCAAAG AGGAAGATCT CAAGAAATAT CCTCCACCTG CGAAAGAGCC TTCACTGAAC 
ACAGATGATG CGATGGACAT ACTTTCAGGA GATTTCACGA CCCCTGCATC GGCACCTGTT 
GTCAAAGCTC CTGTTCCTCC TGCACAGGAC ACAAAGAAGC ATGCTGCAGT GCCTGAGAAG 
ACCAAAGATA TTCCCAAGAC TAAAGCTGAT GCTCTGTCAA GTGATTTCGT GGCTCCGGCA 
CATTCTGCTC CTAAGGTTTC TTCAGCTGCT CCTAAAACCA TCCCTCCAGG CCCACCACAA 
ACCCCAGATG AGGATCCTTT CAGTGCTTTG GGCGACACTC TTTCTGCCCC AGAACCACCA 
AAAAAACAAC CTGACCTGAA ACCTAAGGAC ATCGTACATG AGAAGGATGT GACGTCAAAG 
AAAGGAGTTC GTGTTGGAGA AAGAGACGAC ACACTCCCAC CAGAATACAG ATTCAAAGAG 
GAAGATCTCA AGAAATATCC TCCTCCTCAG AAAGATCCTT CCTTAGATGC TAATGAAGCT 
CTGGATATTC TGTCTGGGGA TTTTACAACT CCTTCCGCAC CATCGGCTGT TTCCCCTGCC 
TCTAAATCCT CTGCCAAGTC TTCAGATTCA GCTTTAGATG CTCTTGCTGG TGATTTCGTC 
GCTTCTTCTT CTGCGTCTTC AGTTCATTCT GCCGTCTCTG GCCCCCCACG CTCCGACAGA 
CTGTTGTCAG ACGGAACCTC ATCAGCTCTA GATGCCCTGT CAGAAACTCT GGGAGACATT 
AAAGCAGCCC CTGAACCCGC CCCTGTCCTA CCTAAAACTG TCGTTAAGGA GAAGACGGTC 
ATAGAAGAGA GAGTGAGTAA ACCGGGTGAG AGAGACGACT CCCTTCCACC AGATCAGCGG 
TTCACTGCGG AGGATCGCAA GGCATTTGAA GAAGCAAAGA AGAAAGACGC CAAACCAAAA 
CAGCCATCAA TGGACGATTC AGCGGCTCTG GACATGTTGT CTAGTGATTT TTCAGATGTA 
CCGACTGTAA AGCCCACTGT ATCTGATGGC AAAAGCTTCA CCCCTGAACC AACACCACCA 
ACCTTTAAGG CAACCGGTCC GGTTTTAGAC AAACTGGCAG ACAACGTGCT TTCCAACCTG 
ACTGACCCCA AAGCCAAAGA CAGCAAAGCA AAGGGAAAGG GGGCGAAACC AAAGTCTAAA 
CCAAAGAAAC AGGCAGTGGA AGACTCTTCA GCCACAGATC TGCTGTCCAG TAAACCGAGC 
TCAGATGTGG TGCCTTCAGC TTCCACAAAG GCTGGAAAAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324551.1
CDS	68..2272
Translation	MGQIFSWIRGTRDQPALQDIAVEQQVSLTGYYPIESQQVTPTTTSQVSTVKPAQYEVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_021468876.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468876.1

ORF Insert Sequence:

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGGTCTC GCTTACTGGA TATTATCCTA TCGAGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGGT GCAGGTTGAA 
GTGGGTCCTT CAGCAGCAAA AGTCAGTGCA GATGCTGACG ATCCGTTTGA TGCCCTGTCT 
GGCACATTAC CATCATCACA ACCTTTGGCT CCTAAAGTCC ACTACACTGG ACCTGAGATC 
AAAGAGCCCA AACTGAAATC AGAGAAAGGT GTTTTGTGTG GTGAGCGAGA TGACACGCTG 
CCGCCCGGAT ACAGAAAGGA AGACCTGGAA AAGAAGCATG GAGCTGGAGT TCCAGCTGGA 
GTTCCGGAGA AGCCTAAAGA AGTTCCCAAG CCCATAAGCA CAGATGATGC ACTGGAATCT 
CTCTCCTCTA CGTTTGTGTC TCCAGCTCAA GCTGCTCCAA AGAAGACAGA CGTGAAAACT 
GAAACAATAG GAGCAGTGGA TGTTAAATCG GCAGGCACCA CTAACTTTGC TCCCCCTCCA 
CCATCTCAGC AGAAACAACC GGCGTCGTCT CAAGCTGCTG CTGTAACCAA ATCACCTGCT 
CCACCGGCAG ACAAGAAAGC TAAAATGGAG ACTCATGCAC AACCAGCCAA AGCAAAGACA 
GAGTCGGACA GCTCCATATC GCTGGACGCT CTCGATGCTT TGAGCGACAC ACTGGGGGCT 
TCTAAACCAC CCCAAAAATT ACCAGAACTC AAACCTGGAC AGATAGTTGA TGAGAAGAAA 
CAGACCTCAG AGAAAGGTGT TCGTGTTGGA GAAAGAGACG ACACACTTCC ACCACAATAC 
AGATTCAAAG AGGAAGATCT CAAGAAATAT CCTCCACCTG CGAAAGAGCC TTCACTGAAC 
ACAGATGATG CGATGGACAT ACTTTCAGGA GATTTCACGA CCCCTGCATC GGCACCTGTT 
GTCAAAGCTC CTGTTCCTCC TGCACAGGAC ACAAAGAAGC ATGCTGCAGT GCCTGAGAAG 
ACCAAAGATA TTCCCAAGAC TAAAGCTGAT GCTCTGTCAA GTGATTTCGT GGCTCCGGCA 
CATTCTGCTC CTAAGGTTTC TTCAGCTGCT CCTAAAACCA TCCCTCCAGG CCCACCACAA 
ACCCCAGATG AGGATCCTTT CAGTGCTTTG GGCGACACTC TTTCTGCCCC AGAACCACCA 
AAAAAACAAC CTGACCTGAA ACCTAAGGAC ATCGTACATG AGAAGGATGT GACGTCAAAG 
AAAGGAGTTC GTGTTGGAGA AAGAGACGAC ACACTCCCAC CAGAATACAG ATTCAAAGAG 
GAAGATCTCA AGAAATATCC TCCTCCTCAG AAAGATCCTT CCTTAGATGC TAATGAAGCT 
CTGGATATTC TGTCTGGGGA TTTTACAACT CCTTCCGCAC CATCGGCTGT TTCCCCTGCC 
TCTAAATCCT CTGCCAAGTC TTCAGATTCA GCTTTAGATG CTCTTGCTGG TGATTTCGTC 
GCTTCTTCTT CTGCGTCTTC AGTTCATTCT GCCGTCTCTG GCCCCCCACG CTCCGACAGA 
CTGTTGTCAG ACGGAACCTC ATCAGCTCTA GATGCCCTGT CAGAAACTCT GGGAGACATT 
AAAGCAGCCC CTGAACCCGC CCCTGTCCTA CCTAAAACTG TCGTTAAGGA GAAGACGGTC 
ATAGAAGAGA GAGTGAGTAA ACCGGGTGAG AGAGACGACT CCCTTCCACC AGATCAGCGG 
TTCACTGCGG AGGATCGCAA GGCATTTGAA GAAGCAAAGA AGAAAGACGC CAAACCAAAA 
CAGCCATCAA TGGACGATTC AGCGGCTCTG GACATGTTGT CTAGTGATTT TTCAGATGTA 
CCGACTGTAA AGCCCACTGT ATCTGATGGC AAAAGCTTCA CCCCTGAACC AACACCACCA 
ACCTTTAAGG CAACCGGTCC GGTTTTAGAC AAACTGGCAG ACAACGTGCT TTCCAACCTG 
ACTGACCCCA AAGCCAAAGA CAGCAAAGCA AAGGGAAAGG GGGCGAAACC AAAGTCTAAA 
CCAAAGAAAC AGGCAGTGGA AGACTCTTCA GCCACAGATC TGCTGTCCAG TAAACCGAGC 
TCAGATGTGG TGCCTTCAGC TTCCACAAAG GCTGGAAAAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_021468878.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324553.1 Danio rerio calpastatin (cast), transcript variant X25, mRNA.

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CloneID	ODa150928
Clone ID Related Accession (Same CDS sequence)	XM_021468878.1
Accession Version	XM_021468878.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calpastatin isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGGTCTC GCTTACTGGA TATTATCCTA TCGAGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGGT GCAGGTTGAA 
GTGGGTCCTT CAGCAGCAAA AGCTGACGAT CCGTTTGATG CCCTGTCTGG CACATTACCA 
TCATCACAAC CTTTGGCTCC TAAAGTCCAC TACACTGGAC CTGAGATCAA AGAGCCCAAA 
CTGAAATCAG AGAAAGGTGT TTTGTGTGGT GAGCGAGATG ACACGCTGCC GCCCGGATAC 
AGAAAGGAAG ACCTGGAAAA GAAGCATGGA GCTGGAGTTC CAGCTGGAGT TCCGGAGAAG 
CCTAAAGAAG TTCCCAAGCC CATAAGCACA GATGATGCAC TGGAATCTCT CTCCTCTACG 
TTTGTGTCTC CAGCTCAAGC TGCTCCAAAG AAGACAGACG TGAAAACTGA AACAATAGGA 
GCAGTGGATG TTAAATCGGC AGGCACCACT AACTTTGCTC CCCCTCCACC ATCTCAGCAG 
AAACAACCGG CGTCGTCTCA AGCTGCTGCT GTAACCAAAT CACCTGCTCC ACCGGCAGAC 
AAGAAAGCTA AAATGGAGAC TCATGCACAA CCAGCCAAAG CAAAGACAGA GTCGGACAGC 
TCCATATCGC TGGACGCTCT CGATGCTTTG AGCGACACAC TGGGGGCTTC TAAACCACCC 
CAAAAATTAC CAGAACTCAA ACCTGGACAG ATAGTTGATG AGAAGAAACA GACCTCAGAG 
AAAGGTGTTC GTGTTGGAGA AAGAGACGAC ACACTTCCAC CACAATACAG ATTCAAAGAG 
GAAGATCTCA AGAAATATCC TCCACCTGCG AAAGAGCCTT CACTGAACAC AGATGATGCG 
ATGGACATAC TTTCAGGAGA TTTCACGACC CCTGCATCGG CACCTGTTGT CAAAGCTCCT 
GTTCCTCCTG CACAGGACAC AAAGAAGCAT GCTGCAGTGC CTGAGAAGAC CAAAGATATT 
CCCAAGACTA AAGCTGATGC TCTGTCAAGT GATTTCGTGG CTCCGGCACA TTCTGCTCCT 
AAGGTTTCTT CAGCTGCTCC TAAAACCATC CCTCCAGGCC CACCACAAAC CCCAGATGAG 
GATCCTTTCA GTGCTTTGGG CGACACTCTT TCTGCCCCAG AACCACCAAA AAAACAACCT 
GACCTGAAAC CTAAGGACAT CGTACATGAG AAGGATGTGA CGTCAAAGAA AGGAGTTCGT 
GTTGGAGAAA GAGACGACAC ACTCCCACCA GAATACAGAT TCAAAGAGGA AGATCTCAAG 
AAATATCCTC CTCCTCAGAA AGATCCTTCC TTAGATGCTA ATGAAGCTCT GGATATTCTG 
TCTGGGGATT TTACAACTCC TTCCGCACCA TCGGCTGTTT CCCCTGCCTC TAAATCCTCT 
GCCAAGTCTT CAGATTCAGC TTTAGATGCT CTTGCTGGTG ATTTCGTCGC TTCTTCTTCT 
GCGTCTTCAG TTCATTCTGC CGTCTCTGGC CCCCCACGCT CCGACAGACT GTTGTCAGAC 
GGAACCTCAT CAGCTCTAGA TGCCCTGTCA GAAACTCTGG GAGACATTAA AGCAGCCCCT 
GAACCCGCCC CTGTCCTACC TAAAACTGTC GTTAAGGAGA AGACGGTCAT AGAAGAGAGA 
GTGAGTAAAC CGGGTGAGAG AGACGACTCC CTTCCACCAG ATCAGCGGTT CACTGCGGAG 
GATCGCAAGG CATTTGAAGA AGCAAAGAAG AAAGACGCCA AACCAAAACA GCCATCAATG 
GACGATTCAG CGGCTCTGGA CATGTTGTCT AGTGATTTTT CAGATGTACC GACTGTAAAG 
CCCACTGTAT CTGATGGCAA AAGCTTCACC CCTGAACCAA CACCACCAAC CTTTAAGGCA 
ACCGGTCCGG TTTTAGACAA ACTGGCAGAC AACGTGCTTT CCAACCTGAC TGACCCCAAA 
GCCAAAGACA GCAAAGCAAA GGGAAAGGGG GCGAAACCAA AGTCTAAACC AAAGAAACAG 
GCAGTGGAAG ACTCTTCAGC CACAGATCTG CTGTCCAGTA AACCGAGCTC AGATGTGGTG 
CCTTCAGCTT CCACAAAGGC TGGAAAAAGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324553.1
CDS	69..2261
Translation	MGQIFSWIRGTRDQPALQDIAVEQQVSLTGYYPIESQQVTPTTTSQVSTVKPAQYEVQVEVGPSAAKADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_021468878.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468878.1

ORF Insert Sequence:

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGGTCTC GCTTACTGGA TATTATCCTA TCGAGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGGT GCAGGTTGAA 
GTGGGTCCTT CAGCAGCAAA AGCTGACGAT CCGTTTGATG CCCTGTCTGG CACATTACCA 
TCATCACAAC CTTTGGCTCC TAAAGTCCAC TACACTGGAC CTGAGATCAA AGAGCCCAAA 
CTGAAATCAG AGAAAGGTGT TTTGTGTGGT GAGCGAGATG ACACGCTGCC GCCCGGATAC 
AGAAAGGAAG ACCTGGAAAA GAAGCATGGA GCTGGAGTTC CAGCTGGAGT TCCGGAGAAG 
CCTAAAGAAG TTCCCAAGCC CATAAGCACA GATGATGCAC TGGAATCTCT CTCCTCTACG 
TTTGTGTCTC CAGCTCAAGC TGCTCCAAAG AAGACAGACG TGAAAACTGA AACAATAGGA 
GCAGTGGATG TTAAATCGGC AGGCACCACT AACTTTGCTC CCCCTCCACC ATCTCAGCAG 
AAACAACCGG CGTCGTCTCA AGCTGCTGCT GTAACCAAAT CACCTGCTCC ACCGGCAGAC 
AAGAAAGCTA AAATGGAGAC TCATGCACAA CCAGCCAAAG CAAAGACAGA GTCGGACAGC 
TCCATATCGC TGGACGCTCT CGATGCTTTG AGCGACACAC TGGGGGCTTC TAAACCACCC 
CAAAAATTAC CAGAACTCAA ACCTGGACAG ATAGTTGATG AGAAGAAACA GACCTCAGAG 
AAAGGTGTTC GTGTTGGAGA AAGAGACGAC ACACTTCCAC CACAATACAG ATTCAAAGAG 
GAAGATCTCA AGAAATATCC TCCACCTGCG AAAGAGCCTT CACTGAACAC AGATGATGCG 
ATGGACATAC TTTCAGGAGA TTTCACGACC CCTGCATCGG CACCTGTTGT CAAAGCTCCT 
GTTCCTCCTG CACAGGACAC AAAGAAGCAT GCTGCAGTGC CTGAGAAGAC CAAAGATATT 
CCCAAGACTA AAGCTGATGC TCTGTCAAGT GATTTCGTGG CTCCGGCACA TTCTGCTCCT 
AAGGTTTCTT CAGCTGCTCC TAAAACCATC CCTCCAGGCC CACCACAAAC CCCAGATGAG 
GATCCTTTCA GTGCTTTGGG CGACACTCTT TCTGCCCCAG AACCACCAAA AAAACAACCT 
GACCTGAAAC CTAAGGACAT CGTACATGAG AAGGATGTGA CGTCAAAGAA AGGAGTTCGT 
GTTGGAGAAA GAGACGACAC ACTCCCACCA GAATACAGAT TCAAAGAGGA AGATCTCAAG 
AAATATCCTC CTCCTCAGAA AGATCCTTCC TTAGATGCTA ATGAAGCTCT GGATATTCTG 
TCTGGGGATT TTACAACTCC TTCCGCACCA TCGGCTGTTT CCCCTGCCTC TAAATCCTCT 
GCCAAGTCTT CAGATTCAGC TTTAGATGCT CTTGCTGGTG ATTTCGTCGC TTCTTCTTCT 
GCGTCTTCAG TTCATTCTGC CGTCTCTGGC CCCCCACGCT CCGACAGACT GTTGTCAGAC 
GGAACCTCAT CAGCTCTAGA TGCCCTGTCA GAAACTCTGG GAGACATTAA AGCAGCCCCT 
GAACCCGCCC CTGTCCTACC TAAAACTGTC GTTAAGGAGA AGACGGTCAT AGAAGAGAGA 
GTGAGTAAAC CGGGTGAGAG AGACGACTCC CTTCCACCAG ATCAGCGGTT CACTGCGGAG 
GATCGCAAGG CATTTGAAGA AGCAAAGAAG AAAGACGCCA AACCAAAACA GCCATCAATG 
GACGATTCAG CGGCTCTGGA CATGTTGTCT AGTGATTTTT CAGATGTACC GACTGTAAAG 
CCCACTGTAT CTGATGGCAA AAGCTTCACC CCTGAACCAA CACCACCAAC CTTTAAGGCA 
ACCGGTCCGG TTTTAGACAA ACTGGCAGAC AACGTGCTTT CCAACCTGAC TGACCCCAAA 
GCCAAAGACA GCAAAGCAAA GGGAAAGGGG GCGAAACCAA AGTCTAAACC AAAGAAACAG 
GCAGTGGAAG ACTCTTCAGC CACAGATCTG CTGTCCAGTA AACCGAGCTC AGATGTGGTG 
CCTTCAGCTT CCACAAAGGC TGGAAAAAGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_021468879.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324554.1 Danio rerio calpastatin (cast), transcript variant X26, mRNA.

Price & Availability↑

CloneID	ODa150929
Clone ID Related Accession (Same CDS sequence)	XM_021468879.1
Accession Version	XM_021468879.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calpastatin isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTACG CAATGTATTG GGCGCGCAAC AATGGAGACA CTTCATTTCA AAAACAAGAA 
TACGCTCCAC AGACATCAAG GTTCATGTCT CAGCAGGTCA CACCCACCAC AACATCTCAG 
GTCTCCACAG TGAAACCTGC ACAATATGAG GTGCAGGTTG AAGTGGGTCC TTCAGCAGCA 
AAAGTCAGTG CAGATGCTGA CGATCCGTTT GATGCCCTGT CTGGCACATT ACCATCATCA 
CAACCTTTGG CTCCTAAAGT CCACTACACT GGACCTGAGA TCAAAGAGCC CAAACTGAAA 
TCAGAGAAAG GTGTTTTGTG TGGTGAGCGA GATGACACGC TGCCGCCCGG ATACAGAAAG 
GAAGACCTGG AAAAGAAGCA TGGAGCTGGA GTTCCAGCTG GAGTTCCGGA GAAGCCTAAA 
GAAGTTCCCA AGCCCATAAG CACAGATGAT GCACTGGAAT CTCTCTCCTC TACGTTTGTG 
TCTCCAGCTC AAGCTGCTCC AAAGAAGACA GACGTGAAAA CTGAAACAAT AGGAGCAGTG 
GATGTTAAAT CGGCAGGCAC CACTAACTTT GCTCCCCCTC CACCATCTCA GCAGAAACAA 
CCGGCGTCGT CTCAAGCTGC TGCTGTAACC AAATCACCTG CTCCACCGGC AGACAAGAAA 
GCTAAAATGG AGACTCATGC ACAACCAGCC AAAGCAAAGA CAGAGTCGGA CAGCTCCATA 
TCGCTGGACG CTCTCGATGC TTTGAGCGAC ACACTGGGGG CTTCTAAACC ACCCCAAAAA 
TTACCAGAAC TCAAACCTGG ACAGATAGTT GATGAGAAGA AACAGACCTC AGAGAAAGGT 
GTTCGTGTTG GAGAAAGAGA CGACACACTT CCACCACAAT ACAGATTCAA AGAGGAAGAT 
CTCAAGAAAT ATCCTCCACC TGCGAAAGAG CCTTCACTGA ACACAGATGA TGCGATGGAC 
ATACTTTCAG GAGATTTCAC GACCCCTGCA TCGGCACCTG TTGTCAAAGC TCCTGTTCCT 
CCTGCACAGG ACACAAAGAA GCATGCTGCA GTGCCTGAGA AGACCAAAGA TATTCCCAAG 
ACTAAAGCTG ATGCTCTGTC AAGTGATTTC GTGGCTCCGG CACATTCTGC TCCTAAGGTT 
TCTTCAGCTG CTCCTAAAAC CATCCCTCCA GGCCCACCAC AAACCCCAGA TGAGGATCCT 
TTCAGTGCTT TGGGCGACAC TCTTTCTGCC CCAGAACCAC CAAAAAAACA ACCTGACCTG 
AAACCTAAGG ACATCGTACA TGAGAAGGAT GTGACGTCAA AGAAAGGAGT TCGTGTTGGA 
GAAAGAGACG ACACACTCCC ACCAGAATAC AGATTCAAAG AGGAAGATCT CAAGAAATAT 
CCTCCTCCTC AGAAAGATCC TTCCTTAGAT GCTAATGAAG CTCTGGATAT TCTGTCTGGG 
GATTTTACAA CTCCTTCCGC ACCATCGGCT GTTTCCCCTG CCTCTAAATC CTCTGCCAAG 
TCTTCAGATT CAGCTTTAGA TGCTCTTGCT GGTGATTTCG TCGCTTCTTC TTCTGCGTCT 
TCAGTTCATT CTGCCGTCTC TGGCCCCCCA CGCTCCGACA GACTGTTGTC AGACGGAACC 
TCATCAGCTC TAGATGCCCT GTCAGAAACT CTGGGAGACA TTAAAGCAGC CCCTGAACCC 
GCCCCTGTCC TACCTAAAAC TGTCGTTAAG GAGAAGACGG TCATAGAAGA GAGAGTGAGT 
AAACCGGGTG AGAGAGACGA CTCCCTTCCA CCAGATCAGC GGTTCACTGC GGAGGATCGC 
AAGGCATTTG AAGAAGCAAA GAAGAAAGAC GCCAAACCAA AACAGCCATC AATGGACGAT 
TCAGCGGCTC TGGACATGTT GTCTAGTGAT TTTTCAGATG TACCGACTGT AAAGCCCACT 
GTATCTGATG GCAAAAGCTT CACCCCTGAA CCAACACCAC CAACCTTTAA GGCAACCGGT 
CCGGTTTTAG ACAAACTGGC AGACAACGTG CTTTCCAACC TGACTGACCC CAAAGCCAAA 
GACAGCAAAG CAAAGGGAAA GGGGGCGAAA CCAAAGTCTA AACCAAAGAA ACAGGCAGTG 
GAAGACTCTT CAGCCACAGA TCTGCTGTCC AGTAAACCGA GCTCAGATGT GGTGCCTTCA 
GCTTCCACAA AGGCTGGAAA AAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324554.1
CDS	111..2297
Translation	MAYAMYWARNNGDTSFQKQEYAPQTSRFMSQQVTPTTTSQVSTVKPAQYEVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_021468879.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468879.1

ORF Insert Sequence:

ATGGCGTACG CAATGTATTG GGCGCGCAAC AATGGAGACA CTTCATTTCA AAAACAAGAA 
TACGCTCCAC AGACATCAAG GTTCATGTCT CAGCAGGTCA CACCCACCAC AACATCTCAG 
GTCTCCACAG TGAAACCTGC ACAATATGAG GTGCAGGTTG AAGTGGGTCC TTCAGCAGCA 
AAAGTCAGTG CAGATGCTGA CGATCCGTTT GATGCCCTGT CTGGCACATT ACCATCATCA 
CAACCTTTGG CTCCTAAAGT CCACTACACT GGACCTGAGA TCAAAGAGCC CAAACTGAAA 
TCAGAGAAAG GTGTTTTGTG TGGTGAGCGA GATGACACGC TGCCGCCCGG ATACAGAAAG 
GAAGACCTGG AAAAGAAGCA TGGAGCTGGA GTTCCAGCTG GAGTTCCGGA GAAGCCTAAA 
GAAGTTCCCA AGCCCATAAG CACAGATGAT GCACTGGAAT CTCTCTCCTC TACGTTTGTG 
TCTCCAGCTC AAGCTGCTCC AAAGAAGACA GACGTGAAAA CTGAAACAAT AGGAGCAGTG 
GATGTTAAAT CGGCAGGCAC CACTAACTTT GCTCCCCCTC CACCATCTCA GCAGAAACAA 
CCGGCGTCGT CTCAAGCTGC TGCTGTAACC AAATCACCTG CTCCACCGGC AGACAAGAAA 
GCTAAAATGG AGACTCATGC ACAACCAGCC AAAGCAAAGA CAGAGTCGGA CAGCTCCATA 
TCGCTGGACG CTCTCGATGC TTTGAGCGAC ACACTGGGGG CTTCTAAACC ACCCCAAAAA 
TTACCAGAAC TCAAACCTGG ACAGATAGTT GATGAGAAGA AACAGACCTC AGAGAAAGGT 
GTTCGTGTTG GAGAAAGAGA CGACACACTT CCACCACAAT ACAGATTCAA AGAGGAAGAT 
CTCAAGAAAT ATCCTCCACC TGCGAAAGAG CCTTCACTGA ACACAGATGA TGCGATGGAC 
ATACTTTCAG GAGATTTCAC GACCCCTGCA TCGGCACCTG TTGTCAAAGC TCCTGTTCCT 
CCTGCACAGG ACACAAAGAA GCATGCTGCA GTGCCTGAGA AGACCAAAGA TATTCCCAAG 
ACTAAAGCTG ATGCTCTGTC AAGTGATTTC GTGGCTCCGG CACATTCTGC TCCTAAGGTT 
TCTTCAGCTG CTCCTAAAAC CATCCCTCCA GGCCCACCAC AAACCCCAGA TGAGGATCCT 
TTCAGTGCTT TGGGCGACAC TCTTTCTGCC CCAGAACCAC CAAAAAAACA ACCTGACCTG 
AAACCTAAGG ACATCGTACA TGAGAAGGAT GTGACGTCAA AGAAAGGAGT TCGTGTTGGA 
GAAAGAGACG ACACACTCCC ACCAGAATAC AGATTCAAAG AGGAAGATCT CAAGAAATAT 
CCTCCTCCTC AGAAAGATCC TTCCTTAGAT GCTAATGAAG CTCTGGATAT TCTGTCTGGG 
GATTTTACAA CTCCTTCCGC ACCATCGGCT GTTTCCCCTG CCTCTAAATC CTCTGCCAAG 
TCTTCAGATT CAGCTTTAGA TGCTCTTGCT GGTGATTTCG TCGCTTCTTC TTCTGCGTCT 
TCAGTTCATT CTGCCGTCTC TGGCCCCCCA CGCTCCGACA GACTGTTGTC AGACGGAACC 
TCATCAGCTC TAGATGCCCT GTCAGAAACT CTGGGAGACA TTAAAGCAGC CCCTGAACCC 
GCCCCTGTCC TACCTAAAAC TGTCGTTAAG GAGAAGACGG TCATAGAAGA GAGAGTGAGT 
AAACCGGGTG AGAGAGACGA CTCCCTTCCA CCAGATCAGC GGTTCACTGC GGAGGATCGC 
AAGGCATTTG AAGAAGCAAA GAAGAAAGAC GCCAAACCAA AACAGCCATC AATGGACGAT 
TCAGCGGCTC TGGACATGTT GTCTAGTGAT TTTTCAGATG TACCGACTGT AAAGCCCACT 
GTATCTGATG GCAAAAGCTT CACCCCTGAA CCAACACCAC CAACCTTTAA GGCAACCGGT 
CCGGTTTTAG ACAAACTGGC AGACAACGTG CTTTCCAACC TGACTGACCC CAAAGCCAAA 
GACAGCAAAG CAAAGGGAAA GGGGGCGAAA CCAAAGTCTA AACCAAAGAA ACAGGCAGTG 
GAAGACTCTT CAGCCACAGA TCTGCTGTCC AGTAAACCGA GCTCAGATGT GGTGCCTTCA 
GCTTCCACAA AGGCTGGAAA AAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_021468880.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324555.1 Danio rerio calpastatin (cast), transcript variant X28, mRNA.

Price & Availability↑

CloneID	ODa150930
Clone ID Related Accession (Same CDS sequence)	XM_021468880.1
Accession Version	XM_021468880.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calpastatin isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGTCTCA GCAGGTCACA CCCACCACAA CATCTCAGGT CTCCACAGTG 
AAACCTGCAC AATATGAGGT GCAGGTTGAA GTGGGTCCTT CAGCAGCAAA AGCTGACGAT 
CCGTTTGATG CCCTGTCTGG CACATTACCA TCATCACAAC CTTTGGCTCC TAAAGTCCAC 
TACACTGGAC CTGAGATCAA AGAGCCCAAA CTGAAATCAG AGAAAGGTGT TTTGTGTGGT 
GAGCGAGATG ACACGCTGCC GCCCGGATAC AGAAAGGAAG ACCTGGAAAA GAAGCATGGA 
GCTGGAGTTC CAGCTGGAGT TCCGGAGAAG CCTAAAGAAG TTCCCAAGCC CATAAGCACA 
GATGATGCAC TGGAATCTCT CTCCTCTACG TTTGTGTCTC CAGCTCAAGC TGCTCCAAAG 
AAGACAGACG TGAAAACTGA AACAATAGGA GCAGTGGATG TTAAATCGGC AGGCACCACT 
AACTTTGCTC CCCCTCCACC ATCTCAGCAG AAACAACCGG CGTCGTCTCA AGCTGCTGCT 
GTAACCAAAT CACCTGCTCC ACCGGCAGAC AAGAAAGCTA AAATGGAGAC TCATGCACAA 
CCAGCCAAAG CAAAGACAGA GTCGGACAGC TCCATATCGC TGGACGCTCT CGATGCTTTG 
AGCGACACAC TGGGGGCTTC TAAACCACCC CAAAAATTAC CAGAACTCAA ACCTGGACAG 
ATAGTTGATG AGAAGAAACA GACCTCAGAG AAAGGTGTTC GTGTTGGAGA AAGAGACGAC 
ACACTTCCAC CACAATACAG ATTCAAAGAG GAAGATCTCA AGAAATATCC TCCACCTGCG 
AAAGAGCCTT CACTGAACAC AGATGATGCG ATGGACATAC TTTCAGGAGA TTTCACGACC 
CCTGCATCGG CACCTGTTGT CAAAGCTCCT GTTCCTCCTG CACAGGACAC AAAGAAGCAT 
GCTGCAGTGC CTGAGAAGAC CAAAGATATT CCCAAGACTA AAGCTGATGC TCTGTCAAGT 
GATTTCGTGG CTCCGGCACA TTCTGCTCCT AAGGTTTCTT CAGCTGCTCC TAAAACCATC 
CCTCCAGGCC CACCACAAAC CCCAGATGAG GATCCTTTCA GTGCTTTGGG CGACACTCTT 
TCTGCCCCAG AACCACCAAA AAAACAACCT GACCTGAAAC CTAAGGACAT CGTACATGAG 
AAGGATGTGA CGTCAAAGAA AGGAGTTCGT GTTGGAGAAA GAGACGACAC ACTCCCACCA 
GAATACAGAT TCAAAGAGGA AGATCTCAAG AAATATCCTC CTCCTCAGAA AGATCCTTCC 
TTAGATGCTA ATGAAGCTCT GGATATTCTG TCTGGGGATT TTACAACTCC TTCCGCACCA 
TCGGCTGTTT CCCCTGCCTC TAAATCCTCT GCCAAGTCTT CAGATTCAGC TTTAGATGCT 
CTTGCTGGTG ATTTCGTCGC TTCTTCTTCT GCGTCTTCAG TTCATTCTGC CGTCTCTGGC 
CCCCCACGCT CCGACAGACT GTTGTCAGAC GGAACCTCAT CAGCTCTAGA TGCCCTGTCA 
GAAACTCTGG GAGACATTAA AGCAGCCCCT GAACCCGCCC CTGTCCTACC TAAAACTGTC 
GTTAAGGAGA AGACGGTCAT AGAAGAGAGA GTGAGTAAAC CGGGTGAGAG AGACGACTCC 
CTTCCACCAG ATCAGCGGTT CACTGCGGAG GATCGCAAGG CATTTGAAGA AGCAAAGAAG 
AAAGACGCCA AACCAAAACA GCCATCAATG GACGATTCAG CGGCTCTGGA CATGTTGTCT 
AGTGATTTTT CAGATGTACC GACTGTAAAG CCCACTGTAT CTGATGGCAA AAGCTTCACC 
CCTGAACCAA CACCACCAAC CTTTAAGGCA ACCGGTCCGG TTTTAGACAA ACTGGCAGAC 
AACGTGCTTT CCAACCTGAC TGACCCCAAA GCCAAAGACA GCAAAGCAAA GGGAAAGGGG 
GCGAAACCAA AGTCTAAACC AAAGAAACAG GCAGTGGAAG ACTCTTCAGC CACAGATCTG 
CTGTCCAGTA AACCGAGCTC AGATGTGGTG CCTTCAGCTT CCACAAAGGC TGGAAAAAGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324555.1
CDS	72..2234
Translation	MGQIFSWIRGTRDQPALQDIAVEQQSQQVTPTTTSQVSTVKPAQYEVQVEVGPSAAKADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_021468880.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468880.1

ORF Insert Sequence:

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGTCTCA GCAGGTCACA CCCACCACAA CATCTCAGGT CTCCACAGTG 
AAACCTGCAC AATATGAGGT GCAGGTTGAA GTGGGTCCTT CAGCAGCAAA AGCTGACGAT 
CCGTTTGATG CCCTGTCTGG CACATTACCA TCATCACAAC CTTTGGCTCC TAAAGTCCAC 
TACACTGGAC CTGAGATCAA AGAGCCCAAA CTGAAATCAG AGAAAGGTGT TTTGTGTGGT 
GAGCGAGATG ACACGCTGCC GCCCGGATAC AGAAAGGAAG ACCTGGAAAA GAAGCATGGA 
GCTGGAGTTC CAGCTGGAGT TCCGGAGAAG CCTAAAGAAG TTCCCAAGCC CATAAGCACA 
GATGATGCAC TGGAATCTCT CTCCTCTACG TTTGTGTCTC CAGCTCAAGC TGCTCCAAAG 
AAGACAGACG TGAAAACTGA AACAATAGGA GCAGTGGATG TTAAATCGGC AGGCACCACT 
AACTTTGCTC CCCCTCCACC ATCTCAGCAG AAACAACCGG CGTCGTCTCA AGCTGCTGCT 
GTAACCAAAT CACCTGCTCC ACCGGCAGAC AAGAAAGCTA AAATGGAGAC TCATGCACAA 
CCAGCCAAAG CAAAGACAGA GTCGGACAGC TCCATATCGC TGGACGCTCT CGATGCTTTG 
AGCGACACAC TGGGGGCTTC TAAACCACCC CAAAAATTAC CAGAACTCAA ACCTGGACAG 
ATAGTTGATG AGAAGAAACA GACCTCAGAG AAAGGTGTTC GTGTTGGAGA AAGAGACGAC 
ACACTTCCAC CACAATACAG ATTCAAAGAG GAAGATCTCA AGAAATATCC TCCACCTGCG 
AAAGAGCCTT CACTGAACAC AGATGATGCG ATGGACATAC TTTCAGGAGA TTTCACGACC 
CCTGCATCGG CACCTGTTGT CAAAGCTCCT GTTCCTCCTG CACAGGACAC AAAGAAGCAT 
GCTGCAGTGC CTGAGAAGAC CAAAGATATT CCCAAGACTA AAGCTGATGC TCTGTCAAGT 
GATTTCGTGG CTCCGGCACA TTCTGCTCCT AAGGTTTCTT CAGCTGCTCC TAAAACCATC 
CCTCCAGGCC CACCACAAAC CCCAGATGAG GATCCTTTCA GTGCTTTGGG CGACACTCTT 
TCTGCCCCAG AACCACCAAA AAAACAACCT GACCTGAAAC CTAAGGACAT CGTACATGAG 
AAGGATGTGA CGTCAAAGAA AGGAGTTCGT GTTGGAGAAA GAGACGACAC ACTCCCACCA 
GAATACAGAT TCAAAGAGGA AGATCTCAAG AAATATCCTC CTCCTCAGAA AGATCCTTCC 
TTAGATGCTA ATGAAGCTCT GGATATTCTG TCTGGGGATT TTACAACTCC TTCCGCACCA 
TCGGCTGTTT CCCCTGCCTC TAAATCCTCT GCCAAGTCTT CAGATTCAGC TTTAGATGCT 
CTTGCTGGTG ATTTCGTCGC TTCTTCTTCT GCGTCTTCAG TTCATTCTGC CGTCTCTGGC 
CCCCCACGCT CCGACAGACT GTTGTCAGAC GGAACCTCAT CAGCTCTAGA TGCCCTGTCA 
GAAACTCTGG GAGACATTAA AGCAGCCCCT GAACCCGCCC CTGTCCTACC TAAAACTGTC 
GTTAAGGAGA AGACGGTCAT AGAAGAGAGA GTGAGTAAAC CGGGTGAGAG AGACGACTCC 
CTTCCACCAG ATCAGCGGTT CACTGCGGAG GATCGCAAGG CATTTGAAGA AGCAAAGAAG 
AAAGACGCCA AACCAAAACA GCCATCAATG GACGATTCAG CGGCTCTGGA CATGTTGTCT 
AGTGATTTTT CAGATGTACC GACTGTAAAG CCCACTGTAT CTGATGGCAA AAGCTTCACC 
CCTGAACCAA CACCACCAAC CTTTAAGGCA ACCGGTCCGG TTTTAGACAA ACTGGCAGAC 
AACGTGCTTT CCAACCTGAC TGACCCCAAA GCCAAAGACA GCAAAGCAAA GGGAAAGGGG 
GCGAAACCAA AGTCTAAACC AAAGAAACAG GCAGTGGAAG ACTCTTCAGC CACAGATCTG 
CTGTCCAGTA AACCGAGCTC AGATGTGGTG CCTTCAGCTT CCACAAAGGC TGGAAAAAGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_021468877.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324552.1 Danio rerio calpastatin (cast), transcript variant X24, mRNA.

Price & Availability↑

CloneID	ODa25621
Clone ID Related Accession (Same CDS sequence)	XM_021468877.1
Accession Version	XM_021468877.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calpastatin isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGTCTCA GCAGGTCACA CCCACCACAA CATCTCAGGT CTCCACAGTG 
AAACCTGCAC AATATGAGAA AGGAGCTACA CAATCGTCTA CAGCAGCTGC GACTGTCAAA 
TCCGGCGCTG GAGATCCTGC AACCACCCAG AAAGGATCTT CTCAAGTCAA ACCTCAGGTG 
CAGGTTGAAG TGGGTCCTTC AGCAGCAAAA GTCAGTGCAG ATGCTGACGA TCCGTTTGAT 
GCCCTGTCTG GCACATTACC ATCATCACAA CCTTTGGCTC CTAAAGTCCA CTACACTGGA 
CCTGAGATCA AAGAGGAAAA GAAGCATGGA GCTGGAGTTC CAGCTGGAGT TCCGGAGAAG 
CCTAAAGAAG TTCCCAAGCC CATAAGCACA GATGATGCAC TGGAATCTCT CTCCTCTACG 
TTTGTGTCTC CAGCTCAAGC TGCTCCAAAG AAGACAGACG TGAAAACTGA AACAATAGGA 
GCAGTGGATG TTAAATCGGC AGGCACCACT AACTTTGCTC CCCCTCCACC ATCTCAGCAG 
AAACAACCGG CGTCGTCTCA AGCTGCTGCT GTAACCAAAT CACCTGCTCC ACCGGCAGAC 
AAGAAAGCTA AAATGGAGAC TCATGCACAA CCAGCCAAAG CAAAGACAGA GTCGGACAGC 
TCCATATCGC TGGACGCTCT CGATGCTTTG AGCGACACAC TGGGGGCTTC TAAACCACCC 
CAAAAATTAC CAGAACTCAA ACCTGGACAG ATAGTTGATG AGAAGAAACA GACCTCAGAG 
AAAGGTGTTC GTGTTGGAGA AAGAGACGAC ACACTTCCAC CACAATACAG ATTCAAAGAG 
GAAGATCTCA AGAAATATCC TCCACCTGCG AAAGAGCCTT CACTGAACAC AGATGATGCG 
ATGGACATAC TTTCAGGAGA TTTCACGACC CCTGCATCGG CACCTGTTGT CAAAGCTCCT 
GTTCCTCCTG CACAGGACAC AAAGAAGCAT GCTGCAGTGC CTGAGAAGAC CAAAGATATT 
CCCAAGACTA AAGCTGATGC TCTGTCAAGT GATTTCGTGG CTCCGGCACA TTCTGCTCCT 
AAGGTTTCTT CAGCTGCTCC TAAAACCATC CCTCCAGGCC CACCACAAAC CCCAGATGAG 
GATCCTTTCA GTGCTTTGGG CGACACTCTT TCTGCCCCAG AACCACCAAA AAAACAACCT 
GACCTGAAAC CTAAGGACAT CGTACATGAG AAGGATGTGA CGTCAAAGAA AGGAGTTCGT 
GTTGGAGAAA GAGACGACAC ACTCCCACCA GAATACAGAT TCAAAGAGGA AGATCTCAAG 
AAATATCCTC CTCCTCAGAA AGATCCTTCC TTAGATGCTA ATGAAGCTCT GGATATTCTG 
TCTGGGGATT TTACAACTCC TTCCGCACCA TCGGCTGTTT CCCCTGCCTC TAAATCCTCT 
GCCAAGTCTT CAGATTCAGC TTTAGATGCT CTTGCTGGTG ATTTCGTCGC TTCTTCTTCT 
GCGTCTTCAG TTCATTCTGC CGTCTCTGGC CCCCCACGCT CCGACAGACT GTTGTCAGAC 
GGAACCTCAT CAGCTCTAGA TGCCCTGTCA GAAACTCTGG GAGACATTAA AGCAGCCCCT 
GAACCCGCCC CTGTCCTACC TAAAACTGTC GTTAAGGAGA AGACGGTCAT AGAAGAGAGA 
GTGAGTAAAC CGGGTGAGAG AGACGACTCC CTTCCACCAG ATCAGCGGTT CACTGCGGAG 
GATCGCAAGG CATTTGAAGA AGCAAAGAAG AAAGACGCCA AACCAAAACA GCCATCAATG 
GACGATTCAG CGGCTCTGGA CATGTTGTCT AGTGATTTTT CAGATGTACC GACTGTAAAG 
CCCACTGTAT CTGATGGCAA AAGCTTCACC CCTGAACCAA CACCACCAAC CTTTAAGGCA 
ACCGGTCCGG TTTTAGACAA ACTGGCAGAC AACGTGCTTT CCAACCTGAC TGACCCCAAA 
GCCAAAGACA GCAAAGCAAA GGGAAAGGGG GCGAAACCAA AGTCTAAACC AAAGAAACAG 
GCAGTGGAAG ACTCTTCAGC CACAGATCTG CTGTCCAGTA AACCGAGCTC AGATGTGGTG 
CCTTCAGCTT CCACAAAGGC TGGAAAAAGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324552.1
CDS	72..2264
Translation	MGQIFSWIRGTRDQPALQDIAVEQQSQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_021468877.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468877.1

ORF Insert Sequence:

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGTCTCA GCAGGTCACA CCCACCACAA CATCTCAGGT CTCCACAGTG 
AAACCTGCAC AATATGAGAA AGGAGCTACA CAATCGTCTA CAGCAGCTGC GACTGTCAAA 
TCCGGCGCTG GAGATCCTGC AACCACCCAG AAAGGATCTT CTCAAGTCAA ACCTCAGGTG 
CAGGTTGAAG TGGGTCCTTC AGCAGCAAAA GTCAGTGCAG ATGCTGACGA TCCGTTTGAT 
GCCCTGTCTG GCACATTACC ATCATCACAA CCTTTGGCTC CTAAAGTCCA CTACACTGGA 
CCTGAGATCA AAGAGGAAAA GAAGCATGGA GCTGGAGTTC CAGCTGGAGT TCCGGAGAAG 
CCTAAAGAAG TTCCCAAGCC CATAAGCACA GATGATGCAC TGGAATCTCT CTCCTCTACG 
TTTGTGTCTC CAGCTCAAGC TGCTCCAAAG AAGACAGACG TGAAAACTGA AACAATAGGA 
GCAGTGGATG TTAAATCGGC AGGCACCACT AACTTTGCTC CCCCTCCACC ATCTCAGCAG 
AAACAACCGG CGTCGTCTCA AGCTGCTGCT GTAACCAAAT CACCTGCTCC ACCGGCAGAC 
AAGAAAGCTA AAATGGAGAC TCATGCACAA CCAGCCAAAG CAAAGACAGA GTCGGACAGC 
TCCATATCGC TGGACGCTCT CGATGCTTTG AGCGACACAC TGGGGGCTTC TAAACCACCC 
CAAAAATTAC CAGAACTCAA ACCTGGACAG ATAGTTGATG AGAAGAAACA GACCTCAGAG 
AAAGGTGTTC GTGTTGGAGA AAGAGACGAC ACACTTCCAC CACAATACAG ATTCAAAGAG 
GAAGATCTCA AGAAATATCC TCCACCTGCG AAAGAGCCTT CACTGAACAC AGATGATGCG 
ATGGACATAC TTTCAGGAGA TTTCACGACC CCTGCATCGG CACCTGTTGT CAAAGCTCCT 
GTTCCTCCTG CACAGGACAC AAAGAAGCAT GCTGCAGTGC CTGAGAAGAC CAAAGATATT 
CCCAAGACTA AAGCTGATGC TCTGTCAAGT GATTTCGTGG CTCCGGCACA TTCTGCTCCT 
AAGGTTTCTT CAGCTGCTCC TAAAACCATC CCTCCAGGCC CACCACAAAC CCCAGATGAG 
GATCCTTTCA GTGCTTTGGG CGACACTCTT TCTGCCCCAG AACCACCAAA AAAACAACCT 
GACCTGAAAC CTAAGGACAT CGTACATGAG AAGGATGTGA CGTCAAAGAA AGGAGTTCGT 
GTTGGAGAAA GAGACGACAC ACTCCCACCA GAATACAGAT TCAAAGAGGA AGATCTCAAG 
AAATATCCTC CTCCTCAGAA AGATCCTTCC TTAGATGCTA ATGAAGCTCT GGATATTCTG 
TCTGGGGATT TTACAACTCC TTCCGCACCA TCGGCTGTTT CCCCTGCCTC TAAATCCTCT 
GCCAAGTCTT CAGATTCAGC TTTAGATGCT CTTGCTGGTG ATTTCGTCGC TTCTTCTTCT 
GCGTCTTCAG TTCATTCTGC CGTCTCTGGC CCCCCACGCT CCGACAGACT GTTGTCAGAC 
GGAACCTCAT CAGCTCTAGA TGCCCTGTCA GAAACTCTGG GAGACATTAA AGCAGCCCCT 
GAACCCGCCC CTGTCCTACC TAAAACTGTC GTTAAGGAGA AGACGGTCAT AGAAGAGAGA 
GTGAGTAAAC CGGGTGAGAG AGACGACTCC CTTCCACCAG ATCAGCGGTT CACTGCGGAG 
GATCGCAAGG CATTTGAAGA AGCAAAGAAG AAAGACGCCA AACCAAAACA GCCATCAATG 
GACGATTCAG CGGCTCTGGA CATGTTGTCT AGTGATTTTT CAGATGTACC GACTGTAAAG 
CCCACTGTAT CTGATGGCAA AAGCTTCACC CCTGAACCAA CACCACCAAC CTTTAAGGCA 
ACCGGTCCGG TTTTAGACAA ACTGGCAGAC AACGTGCTTT CCAACCTGAC TGACCCCAAA 
GCCAAAGACA GCAAAGCAAA GGGAAAGGGG GCGAAACCAA AGTCTAAACC AAAGAAACAG 
GCAGTGGAAG ACTCTTCAGC CACAGATCTG CTGTCCAGTA AACCGAGCTC AGATGTGGTG 
CCTTCAGCTT CCACAAAGGC TGGAAAAAGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_021468881.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324556.1 Danio rerio calpastatin (cast), transcript variant X29, mRNA.

Price & Availability↑

CloneID	ODa25623
Clone ID Related Accession (Same CDS sequence)	XM_021468881.1
Accession Version	XM_021468881.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calpastatin isoform X29
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGGTCTC GCTTACTGGA TATTATCCTA TCGAGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGGT GCAGGTTGAA 
GTGGGTCCTT CAGCAGCAAA AGTCAGTGCA GATGCTGACG ATCCGTTTGA TGCCCTGTCT 
GGCACATTAC CATCATCACA ACCTTTGGCT CCTAAAGTCC ACTACACTGG ACCTGAGATC 
AAAGAGGAAA AGAAGCATGG AGCTGGAGTT CCAGCTGGAG TTCCGGAGAA GCCTAAAGAA 
GTTCCCAAGC CCATAAGCAC AGATGATGCA CTGGAATCTC TCTCCTCTAC GTTTGTGTCT 
CCAGCTCAAG CTGCTCCAAA GAAGACAGAC GTGAAAACTG AAACAATAGG AGCAGTGGAT 
GTTAAATCGG CAGGCACCAC TAACTTTGCT CCCCCTCCAC CATCTCAGCA GAAACAACCG 
GCGTCGTCTC AAGCTGCTGC TGTAACCAAA TCACCTGCTC CACCGGCAGA CAAGAAAGCT 
AAAATGGAGA CTCATGCACA ACCAGCCAAA GCAAAGACAG AGTCGGACAG CTCCATATCG 
CTGGACGCTC TCGATGCTTT GAGCGACACA CTGGGGGCTT CTAAACCACC CCAAAAATTA 
CCAGAACTCA AACCTGGACA GATAGTTGAT GAGAAGAAAC AGACCTCAGA GAAAGGTGTT 
CGTGTTGGAG AAAGAGACGA CACACTTCCA CCACAATACA GATTCAAAGA GGAAGATCTC 
AAGAAATATC CTCCACCTGC GAAAGAGCCT TCACTGAACA CAGATGATGC GATGGACATA 
CTTTCAGGAG ATTTCACGAC CCCTGCATCG GCACCTGTTG TCAAAGCTCC TGTTCCTCCT 
GCACAGGACA CAAAGAAGCA TGCTGCAGTG CCTGAGAAGA CCAAAGATAT TCCCAAGACT 
AAAGCTGATG CTCTGTCAAG TGATTTCGTG GCTCCGGCAC ATTCTGCTCC TAAGGTTTCT 
TCAGCTGCTC CTAAAACCAT CCCTCCAGGC CCACCACAAA CCCCAGATGA GGATCCTTTC 
AGTGCTTTGG GCGACACTCT TTCTGCCCCA GAACCACCAA AAAAACAACC TGACCTGAAA 
CCTAAGGACA TCGTACATGA GAAGGATGTG ACGTCAAAGA AAGGAGTTCG TGTTGGAGAA 
AGAGACGACA CACTCCCACC AGAATACAGA TTCAAAGAGG AAGATCTCAA GAAATATCCT 
CCTCCTCAGA AAGATCCTTC CTTAGATGCT AATGAAGCTC TGGATATTCT GTCTGGGGAT 
TTTACAACTC CTTCCGCACC ATCGGCTGTT TCCCCTGCCT CTAAATCCTC TGCCAAGTCT 
TCAGATTCAG CTTTAGATGC TCTTGCTGGT GATTTCGTCG CTTCTTCTTC TGCGTCTTCA 
GTTCATTCTG CCGTCTCTGG CCCCCCACGC TCCGACAGAC TGTTGTCAGA CGGAACCTCA 
TCAGCTCTAG ATGCCCTGTC AGAAACTCTG GGAGACATTA AAGCAGCCCC TGAACCCGCC 
CCTGTCCTAC CTAAAACTGT CGTTAAGGAG AAGACGGTCA TAGAAGAGAG AGTGAGTAAA 
CCGGGTGAGA GAGACGACTC CCTTCCACCA GATCAGCGGT TCACTGCGGA GGATCGCAAG 
GCATTTGAAG AAGCAAAGAA GAAAGACGCC AAACCAAAAC AGCCATCAAT GGACGATTCA 
GCGGCTCTGG ACATGTTGTC TAGTGATTTT TCAGATGTAC CGACTGTAAA GCCCACTGTA 
TCTGATGGCA AAAGCTTCAC CCCTGAACCA ACACCACCAA CCTTTAAGGC AACCGGTCCG 
GTTTTAGACA AACTGGCAGA CAACGTGCTT TCCAACCTGA CTGACCCCAA AGCCAAAGAC 
AGCAAAGCAA AGGGAAAGGG GGCGAAACCA AAGTCTAAAC CAAAGAAACA GGCAGTGGAA 
GACTCTTCAG CCACAGATCT GCTGTCCAGT AAACCGAGCT CAGATGTGGT GCCTTCAGCT 
TCCACAAAGG CTGGAAAAAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324556.1
CDS	68..2191
Translation	MGQIFSWIRGTRDQPALQDIAVEQQVSLTGYYPIESQQVTPTTTSQVSTVKPAQYEVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_021468881.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468881.1

ORF Insert Sequence:

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGGTCTC GCTTACTGGA TATTATCCTA TCGAGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGGT GCAGGTTGAA 
GTGGGTCCTT CAGCAGCAAA AGTCAGTGCA GATGCTGACG ATCCGTTTGA TGCCCTGTCT 
GGCACATTAC CATCATCACA ACCTTTGGCT CCTAAAGTCC ACTACACTGG ACCTGAGATC 
AAAGAGGAAA AGAAGCATGG AGCTGGAGTT CCAGCTGGAG TTCCGGAGAA GCCTAAAGAA 
GTTCCCAAGC CCATAAGCAC AGATGATGCA CTGGAATCTC TCTCCTCTAC GTTTGTGTCT 
CCAGCTCAAG CTGCTCCAAA GAAGACAGAC GTGAAAACTG AAACAATAGG AGCAGTGGAT 
GTTAAATCGG CAGGCACCAC TAACTTTGCT CCCCCTCCAC CATCTCAGCA GAAACAACCG 
GCGTCGTCTC AAGCTGCTGC TGTAACCAAA TCACCTGCTC CACCGGCAGA CAAGAAAGCT 
AAAATGGAGA CTCATGCACA ACCAGCCAAA GCAAAGACAG AGTCGGACAG CTCCATATCG 
CTGGACGCTC TCGATGCTTT GAGCGACACA CTGGGGGCTT CTAAACCACC CCAAAAATTA 
CCAGAACTCA AACCTGGACA GATAGTTGAT GAGAAGAAAC AGACCTCAGA GAAAGGTGTT 
CGTGTTGGAG AAAGAGACGA CACACTTCCA CCACAATACA GATTCAAAGA GGAAGATCTC 
AAGAAATATC CTCCACCTGC GAAAGAGCCT TCACTGAACA CAGATGATGC GATGGACATA 
CTTTCAGGAG ATTTCACGAC CCCTGCATCG GCACCTGTTG TCAAAGCTCC TGTTCCTCCT 
GCACAGGACA CAAAGAAGCA TGCTGCAGTG CCTGAGAAGA CCAAAGATAT TCCCAAGACT 
AAAGCTGATG CTCTGTCAAG TGATTTCGTG GCTCCGGCAC ATTCTGCTCC TAAGGTTTCT 
TCAGCTGCTC CTAAAACCAT CCCTCCAGGC CCACCACAAA CCCCAGATGA GGATCCTTTC 
AGTGCTTTGG GCGACACTCT TTCTGCCCCA GAACCACCAA AAAAACAACC TGACCTGAAA 
CCTAAGGACA TCGTACATGA GAAGGATGTG ACGTCAAAGA AAGGAGTTCG TGTTGGAGAA 
AGAGACGACA CACTCCCACC AGAATACAGA TTCAAAGAGG AAGATCTCAA GAAATATCCT 
CCTCCTCAGA AAGATCCTTC CTTAGATGCT AATGAAGCTC TGGATATTCT GTCTGGGGAT 
TTTACAACTC CTTCCGCACC ATCGGCTGTT TCCCCTGCCT CTAAATCCTC TGCCAAGTCT 
TCAGATTCAG CTTTAGATGC TCTTGCTGGT GATTTCGTCG CTTCTTCTTC TGCGTCTTCA 
GTTCATTCTG CCGTCTCTGG CCCCCCACGC TCCGACAGAC TGTTGTCAGA CGGAACCTCA 
TCAGCTCTAG ATGCCCTGTC AGAAACTCTG GGAGACATTA AAGCAGCCCC TGAACCCGCC 
CCTGTCCTAC CTAAAACTGT CGTTAAGGAG AAGACGGTCA TAGAAGAGAG AGTGAGTAAA 
CCGGGTGAGA GAGACGACTC CCTTCCACCA GATCAGCGGT TCACTGCGGA GGATCGCAAG 
GCATTTGAAG AAGCAAAGAA GAAAGACGCC AAACCAAAAC AGCCATCAAT GGACGATTCA 
GCGGCTCTGG ACATGTTGTC TAGTGATTTT TCAGATGTAC CGACTGTAAA GCCCACTGTA 
TCTGATGGCA AAAGCTTCAC CCCTGAACCA ACACCACCAA CCTTTAAGGC AACCGGTCCG 
GTTTTAGACA AACTGGCAGA CAACGTGCTT TCCAACCTGA CTGACCCCAA AGCCAAAGAC 
AGCAAAGCAA AGGGAAAGGG GGCGAAACCA AAGTCTAAAC CAAAGAAACA GGCAGTGGAA 
GACTCTTCAG CCACAGATCT GCTGTCCAGT AAACCGAGCT CAGATGTGGT GCCTTCAGCT 
TCCACAAAGG CTGGAAAAAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_021468882.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324557.1 Danio rerio calpastatin (cast), transcript variant X32, mRNA.

Price & Availability↑

CloneID	ODa25625
Clone ID Related Accession (Same CDS sequence)	XM_021468882.1
Accession Version	XM_021468882.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calpastatin isoform X32
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGTCTCA GCAGGTCACA CCCACCACAA CATCTCAGGT CTCCACAGTG 
AAACCTGCAC AATATGAGGT GCAGGTTGAA GTGGGTCCTT CAGCAGCAAA AGTCAGTGCA 
GATGCTGACG ATCCGTTTGA TGCCCTGTCT GGCACATTAC CATCATCACA ACCTTTGGCT 
CCTAAAGTCC ACTACACTGG ACCTGAGATC AAAGAGGAAA AGAAGCATGG AGCTGGAGTT 
CCAGCTGGAG TTCCGGAGAA GCCTAAAGAA GTTCCCAAGC CCATAAGCAC AGATGATGCA 
CTGGAATCTC TCTCCTCTAC GTTTGTGTCT CCAGCTCAAG CTGCTCCAAA GAAGACAGAC 
GTGAAAACTG AAACAATAGG AGCAGTGGAT GTTAAATCGG CAGGCACCAC TAACTTTGCT 
CCCCCTCCAC CATCTCAGCA GAAACAACCG GCGTCGTCTC AAGCTGCTGC TGTAACCAAA 
TCACCTGCTC CACCGGCAGA CAAGAAAGCT AAAATGGAGA CTCATGCACA ACCAGCCAAA 
GCAAAGACAG AGTCGGACAG CTCCATATCG CTGGACGCTC TCGATGCTTT GAGCGACACA 
CTGGGGGCTT CTAAACCACC CCAAAAATTA CCAGAACTCA AACCTGGACA GATAGTTGAT 
GAGAAGAAAC AGACCTCAGA GAAAGGTGTT CGTGTTGGAG AAAGAGACGA CACACTTCCA 
CCACAATACA GATTCAAAGA GGAAGATCTC AAGAAATATC CTCCACCTGC GAAAGAGCCT 
TCACTGAACA CAGATGATGC GATGGACATA CTTTCAGGAG ATTTCACGAC CCCTGCATCG 
GCACCTGTTG TCAAAGCTCC TGTTCCTCCT GCACAGGACA CAAAGAAGCA TGCTGCAGTG 
CCTGAGAAGA CCAAAGATAT TCCCAAGACT AAAGCTGATG CTCTGTCAAG TGATTTCGTG 
GCTCCGGCAC ATTCTGCTCC TAAGGTTTCT TCAGCTGCTC CTAAAACCAT CCCTCCAGGC 
CCACCACAAA CCCCAGATGA GGATCCTTTC AGTGCTTTGG GCGACACTCT TTCTGCCCCA 
GAACCACCAA AAAAACAACC TGACCTGAAA CCTAAGGACA TCGTACATGA GAAGGATGTG 
ACGTCAAAGA AAGGAGTTCG TGTTGGAGAA AGAGACGACA CACTCCCACC AGAATACAGA 
TTCAAAGAGG AAGATCTCAA GAAATATCCT CCTCCTCAGA AAGATCCTTC CTTAGATGCT 
AATGAAGCTC TGGATATTCT GTCTGGGGAT TTTACAACTC CTTCCGCACC ATCGGCTGTT 
TCCCCTGCCT CTAAATCCTC TGCCAAGTCT TCAGATTCAG CTTTAGATGC TCTTGCTGGT 
GATTTCGTCG CTTCTTCTTC TGCGTCTTCA GTTCATTCTG CCGTCTCTGG CCCCCCACGC 
TCCGACAGAC TGTTGTCAGA CGGAACCTCA TCAGCTCTAG ATGCCCTGTC AGAAACTCTG 
GGAGACATTA AAGCAGCCCC TGAACCCGCC CCTGTCCTAC CTAAAACTGT CGTTAAGGAG 
AAGACGGTCA TAGAAGAGAG AGTGAGTAAA CCGGGTGAGA GAGACGACTC CCTTCCACCA 
GATCAGCGGT TCACTGCGGA GGATCGCAAG GCATTTGAAG AAGCAAAGAA GAAAGACGCC 
AAACCAAAAC AGCCATCAAT GGACGATTCA GCGGCTCTGG ACATGTTGTC TAGTGATTTT 
TCAGATGTAC CGACTGTAAA GCCCACTGTA TCTGATGGCA AAAGCTTCAC CCCTGAACCA 
ACACCACCAA CCTTTAAGGC AACCGGTCCG GTTTTAGACA AACTGGCAGA CAACGTGCTT 
TCCAACCTGA CTGACCCCAA AGCCAAAGAC AGCAAAGCAA AGGGAAAGGG GGCGAAACCA 
AAGTCTAAAC CAAAGAAACA GGCAGTGGAA GACTCTTCAG CCACAGATCT GCTGTCCAGT 
AAACCGAGCT CAGATGTGGT GCCTTCAGCT TCCACAAAGG CTGGAAAAAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324557.1
CDS	72..2165
Translation	MGQIFSWIRGTRDQPALQDIAVEQQSQQVTPTTTSQVSTVKPAQYEVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_021468882.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X32, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468882.1

ORF Insert Sequence:

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGTCTCA GCAGGTCACA CCCACCACAA CATCTCAGGT CTCCACAGTG 
AAACCTGCAC AATATGAGGT GCAGGTTGAA GTGGGTCCTT CAGCAGCAAA AGTCAGTGCA 
GATGCTGACG ATCCGTTTGA TGCCCTGTCT GGCACATTAC CATCATCACA ACCTTTGGCT 
CCTAAAGTCC ACTACACTGG ACCTGAGATC AAAGAGGAAA AGAAGCATGG AGCTGGAGTT 
CCAGCTGGAG TTCCGGAGAA GCCTAAAGAA GTTCCCAAGC CCATAAGCAC AGATGATGCA 
CTGGAATCTC TCTCCTCTAC GTTTGTGTCT CCAGCTCAAG CTGCTCCAAA GAAGACAGAC 
GTGAAAACTG AAACAATAGG AGCAGTGGAT GTTAAATCGG CAGGCACCAC TAACTTTGCT 
CCCCCTCCAC CATCTCAGCA GAAACAACCG GCGTCGTCTC AAGCTGCTGC TGTAACCAAA 
TCACCTGCTC CACCGGCAGA CAAGAAAGCT AAAATGGAGA CTCATGCACA ACCAGCCAAA 
GCAAAGACAG AGTCGGACAG CTCCATATCG CTGGACGCTC TCGATGCTTT GAGCGACACA 
CTGGGGGCTT CTAAACCACC CCAAAAATTA CCAGAACTCA AACCTGGACA GATAGTTGAT 
GAGAAGAAAC AGACCTCAGA GAAAGGTGTT CGTGTTGGAG AAAGAGACGA CACACTTCCA 
CCACAATACA GATTCAAAGA GGAAGATCTC AAGAAATATC CTCCACCTGC GAAAGAGCCT 
TCACTGAACA CAGATGATGC GATGGACATA CTTTCAGGAG ATTTCACGAC CCCTGCATCG 
GCACCTGTTG TCAAAGCTCC TGTTCCTCCT GCACAGGACA CAAAGAAGCA TGCTGCAGTG 
CCTGAGAAGA CCAAAGATAT TCCCAAGACT AAAGCTGATG CTCTGTCAAG TGATTTCGTG 
GCTCCGGCAC ATTCTGCTCC TAAGGTTTCT TCAGCTGCTC CTAAAACCAT CCCTCCAGGC 
CCACCACAAA CCCCAGATGA GGATCCTTTC AGTGCTTTGG GCGACACTCT TTCTGCCCCA 
GAACCACCAA AAAAACAACC TGACCTGAAA CCTAAGGACA TCGTACATGA GAAGGATGTG 
ACGTCAAAGA AAGGAGTTCG TGTTGGAGAA AGAGACGACA CACTCCCACC AGAATACAGA 
TTCAAAGAGG AAGATCTCAA GAAATATCCT CCTCCTCAGA AAGATCCTTC CTTAGATGCT 
AATGAAGCTC TGGATATTCT GTCTGGGGAT TTTACAACTC CTTCCGCACC ATCGGCTGTT 
TCCCCTGCCT CTAAATCCTC TGCCAAGTCT TCAGATTCAG CTTTAGATGC TCTTGCTGGT 
GATTTCGTCG CTTCTTCTTC TGCGTCTTCA GTTCATTCTG CCGTCTCTGG CCCCCCACGC 
TCCGACAGAC TGTTGTCAGA CGGAACCTCA TCAGCTCTAG ATGCCCTGTC AGAAACTCTG 
GGAGACATTA AAGCAGCCCC TGAACCCGCC CCTGTCCTAC CTAAAACTGT CGTTAAGGAG 
AAGACGGTCA TAGAAGAGAG AGTGAGTAAA CCGGGTGAGA GAGACGACTC CCTTCCACCA 
GATCAGCGGT TCACTGCGGA GGATCGCAAG GCATTTGAAG AAGCAAAGAA GAAAGACGCC 
AAACCAAAAC AGCCATCAAT GGACGATTCA GCGGCTCTGG ACATGTTGTC TAGTGATTTT 
TCAGATGTAC CGACTGTAAA GCCCACTGTA TCTGATGGCA AAAGCTTCAC CCCTGAACCA 
ACACCACCAA CCTTTAAGGC AACCGGTCCG GTTTTAGACA AACTGGCAGA CAACGTGCTT 
TCCAACCTGA CTGACCCCAA AGCCAAAGAC AGCAAAGCAA AGGGAAAGGG GGCGAAACCA 
AAGTCTAAAC CAAAGAAACA GGCAGTGGAA GACTCTTCAG CCACAGATCT GCTGTCCAGT 
AAACCGAGCT CAGATGTGGT GCCTTCAGCT TCCACAAAGG CTGGAAAAAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_017352800.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208289.1 Danio rerio calpastatin (cast), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa56796
Clone ID Related Accession (Same CDS sequence)	XM_017352800.1
Accession Version	XM_017352800.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	calpastatin isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCACA AAAAGAGATG TCATGGTCGT AAGAAAAACA AAAAGGAGAA TGGAGACACT 
TCATTTCAAA AACAAGAATA CGCTCCACAG ACATCAAGGT TCATGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGAA AGGAGCTACA 
CAATCGTCTA CAGCAGCTGC GACTGTCAAA TCCGGCGCTG GAGATCCTGC AACCACCCAG 
AAAGGATCTT CTCAAGTCAA ACCTCAGGTC ACAGATTCTA AATCAACACC TGCATCTTCT 
GCTAAAGTCT CAGCTGTGAG CTCTGGGACT GGAGTTGCTG GTACAACCGG TGGAGCAGGA 
GTGAAACCAG CAGATCCTGC AAAAGATACG CCTCAGAGCA CCACCATTCC TTCAACCAAA 
CTGGGACCTG CTAAGGTGGA TCCGGCTGCT GGGAAGCCCT CTGGCTCAGC TGGTGCCCAA 
AAACAGACAA GTGCTCAAAA GGTGCAGGTT GAAGTGGGTC CTTCAGCAGC AAAAGCTGAC 
GATCCGTTTG ATGCCCTGTC TGGCACATTA CCATCATCAC AACCTTTGGC TCCTAAAGTC 
CACTACACTG GACCTGAGAT CAAAGAGCCC AAACTGAAAT CAGAGAAAGG TGTTTTGTGT 
GGTGAGCGAG ATGACACGCT GCCGCCCGGA TACAGAAAGG AAGACCTGGA AAAGAAGCAT 
GGAGCTGGAG TTCCAGCTGG AGTTCCGGAG AAGCCTAAAG AAGTTCCCAA GCCCATAAGC 
ACAGATGATG CACTGGAATC TCTCTCCTCT ACGTTTGTGT CTCCAGCTCA AGCTGCTCCA 
AAGAAGACAG ACGTGAAAAC TGAAACAATA GGAGCAGTGG ATGTTAAATC GGCAGGCACC 
ACTAACTTTG CTCCCCCTCC ACCATCTCAG CAGAAACAAC CGGCGTCGTC TCAAGCTGCT 
GCTGTAACCA AATCACCTGC TCCACCGGCA GACAAGAAAG CTAAAATGGA GACTCATGCA 
CAACCAGCCA AAGCAAAGAC AGAGTCGGAC AGCTCCATAT CGCTGGACGC TCTCGATGCT 
TTGAGCGACA CACTGGGGGC TTCTAAACCA CCCCAAAAAT TACCAGAACT CAAACCTGGA 
CAGATAGTTG ATGAGAAGAA ACAGACCTCA GAGAAAGGTG TTCGTGTTGG AGAAAGAGAC 
GACACACTTC CACCACAATA CAGATTCAAA GAGGAAGATC TCAAGAAATA TCCTCCACCT 
GCGAAAGAGC CTTCACTGAA CACAGATGAT GCGATGGACA TACTTTCAGG AGATTTCACG 
ACCCCTGCAT CGGCACCTGT TGTCAAAGCT CCTGTTCCTC CTGCACAGGA CACAAAGAAG 
CATGCTGCAG TGCCTGAGAA GACCAAAGAT ATTCCCAAGA CTAAAGCTGA TGCTCTGTCA 
AGTGATTTCG TGGCTCCGGC ACATTCTGCT CCTAAGGTTT CTTCAGCTGC TCCTAAAACC 
ATCCCTCCAG GCCCACCACA AACCCCAGAT GAGGATCCTT TCAGTGCTTT GGGCGACACT 
CTTTCTGCCC CAGAACCACC AAAAAAACAA CCTGACCTGA AACCTAAGGA CATCGTACAT 
GAGAAGGATG TGACGTCAAA GAAAGGAGTT CGTGTTGGAG AAAGAGACGA CACACTCCCA 
CCAGAATACA GATTCAAAGA GGAAGATCTC AAGAAATATC CTCCTCCTCA GAAAGATCCT 
TCCTTAGATG CTAATGAAGC TCTGGATATT CTGTCTGGGG ATTTTACAAC TCCTTCCGCA 
CCATCGGCTG TTTCCCCTGC CTCTAAATCC TCTGCCAAGT CTTCAGATTC AGCTTTAGAT 
GCTCTTGCTG GTGATTTCGT CGCTTCTTCT TCTGCGTCTT CAGTTCATTC TGCCGTCTCT 
GGCCCCCCAC GCTCCGACAG ACTGTTGTCA GACGGAACCT CATCAGCTCT AGATGCCCTG 
TCAGAAACTC TGGGAGACAT TAAAGCAGCC CCTGAACCCG CCCCTGTCCT ACCTAAAACT 
GTCGTTAAGG AGAAGACGGT CATAGAAGAG AGAGTGAGTA AACCGGGTGA GAGAGACGAC 
TCCCTTCCAC CAGATCAGCG GTTCACTGCG GAGGATCGCA AGGCATTTGA AGAAGCAAAG 
AAGAAAGACG CCAAACCAAA ACAGCCATCA ATGGACGATT CAGCGGCTCT GGACATGTTG 
TCTAGTGATT TTTCAGATGT ACCGACTGTA AAGCCCACTG TATCTGATGG CAAAAGCTTC 
ACCCCTGAAC CAACACCACC AACCTTTAAG GCAACCGGTC CGGTTTTAGA CAAACTGGCA 
GACAACGTGC TTTCCAACCT GACTGACCCC AAAGCCAAAG ACAGCAAAGC AAAGGGAAAG 
GGGGCGAAAC CAAAGTCTAA ACCAAAGAAA CAGGCAGTGG AAGACTCTTC AGCCACAGAT 
CTGCTGTCCA GTAAACCGAG CTCAGATGTG GTGCCTTCAG CTTCCACAAA GGCTGGAAAA 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208289.1
CDS	41..2566
Translation	MPHKKRCHGRKKNKKENGDTSFQKQEYAPQTSRFMSQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQVTDSKSTPASSAKVSAVSSGTGVAGTTGGAGVKPADPAKDTPQSTTIPSTKLGPAKVDPAAGKPSGSAGAQKQTSAQKVQVEVGPSAAKADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_017352800.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352800.1

ORF Insert Sequence:

ATGCCGCACA AAAAGAGATG TCATGGTCGT AAGAAAAACA AAAAGGAGAA TGGAGACACT 
TCATTTCAAA AACAAGAATA CGCTCCACAG ACATCAAGGT TCATGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGAA AGGAGCTACA 
CAATCGTCTA CAGCAGCTGC GACTGTCAAA TCCGGCGCTG GAGATCCTGC AACCACCCAG 
AAAGGATCTT CTCAAGTCAA ACCTCAGGTC ACAGATTCTA AATCAACACC TGCATCTTCT 
GCTAAAGTCT CAGCTGTGAG CTCTGGGACT GGAGTTGCTG GTACAACCGG TGGAGCAGGA 
GTGAAACCAG CAGATCCTGC AAAAGATACG CCTCAGAGCA CCACCATTCC TTCAACCAAA 
CTGGGACCTG CTAAGGTGGA TCCGGCTGCT GGGAAGCCCT CTGGCTCAGC TGGTGCCCAA 
AAACAGACAA GTGCTCAAAA GGTGCAGGTT GAAGTGGGTC CTTCAGCAGC AAAAGCTGAC 
GATCCGTTTG ATGCCCTGTC TGGCACATTA CCATCATCAC AACCTTTGGC TCCTAAAGTC 
CACTACACTG GACCTGAGAT CAAAGAGCCC AAACTGAAAT CAGAGAAAGG TGTTTTGTGT 
GGTGAGCGAG ATGACACGCT GCCGCCCGGA TACAGAAAGG AAGACCTGGA AAAGAAGCAT 
GGAGCTGGAG TTCCAGCTGG AGTTCCGGAG AAGCCTAAAG AAGTTCCCAA GCCCATAAGC 
ACAGATGATG CACTGGAATC TCTCTCCTCT ACGTTTGTGT CTCCAGCTCA AGCTGCTCCA 
AAGAAGACAG ACGTGAAAAC TGAAACAATA GGAGCAGTGG ATGTTAAATC GGCAGGCACC 
ACTAACTTTG CTCCCCCTCC ACCATCTCAG CAGAAACAAC CGGCGTCGTC TCAAGCTGCT 
GCTGTAACCA AATCACCTGC TCCACCGGCA GACAAGAAAG CTAAAATGGA GACTCATGCA 
CAACCAGCCA AAGCAAAGAC AGAGTCGGAC AGCTCCATAT CGCTGGACGC TCTCGATGCT 
TTGAGCGACA CACTGGGGGC TTCTAAACCA CCCCAAAAAT TACCAGAACT CAAACCTGGA 
CAGATAGTTG ATGAGAAGAA ACAGACCTCA GAGAAAGGTG TTCGTGTTGG AGAAAGAGAC 
GACACACTTC CACCACAATA CAGATTCAAA GAGGAAGATC TCAAGAAATA TCCTCCACCT 
GCGAAAGAGC CTTCACTGAA CACAGATGAT GCGATGGACA TACTTTCAGG AGATTTCACG 
ACCCCTGCAT CGGCACCTGT TGTCAAAGCT CCTGTTCCTC CTGCACAGGA CACAAAGAAG 
CATGCTGCAG TGCCTGAGAA GACCAAAGAT ATTCCCAAGA CTAAAGCTGA TGCTCTGTCA 
AGTGATTTCG TGGCTCCGGC ACATTCTGCT CCTAAGGTTT CTTCAGCTGC TCCTAAAACC 
ATCCCTCCAG GCCCACCACA AACCCCAGAT GAGGATCCTT TCAGTGCTTT GGGCGACACT 
CTTTCTGCCC CAGAACCACC AAAAAAACAA CCTGACCTGA AACCTAAGGA CATCGTACAT 
GAGAAGGATG TGACGTCAAA GAAAGGAGTT CGTGTTGGAG AAAGAGACGA CACACTCCCA 
CCAGAATACA GATTCAAAGA GGAAGATCTC AAGAAATATC CTCCTCCTCA GAAAGATCCT 
TCCTTAGATG CTAATGAAGC TCTGGATATT CTGTCTGGGG ATTTTACAAC TCCTTCCGCA 
CCATCGGCTG TTTCCCCTGC CTCTAAATCC TCTGCCAAGT CTTCAGATTC AGCTTTAGAT 
GCTCTTGCTG GTGATTTCGT CGCTTCTTCT TCTGCGTCTT CAGTTCATTC TGCCGTCTCT 
GGCCCCCCAC GCTCCGACAG ACTGTTGTCA GACGGAACCT CATCAGCTCT AGATGCCCTG 
TCAGAAACTC TGGGAGACAT TAAAGCAGCC CCTGAACCCG CCCCTGTCCT ACCTAAAACT 
GTCGTTAAGG AGAAGACGGT CATAGAAGAG AGAGTGAGTA AACCGGGTGA GAGAGACGAC 
TCCCTTCCAC CAGATCAGCG GTTCACTGCG GAGGATCGCA AGGCATTTGA AGAAGCAAAG 
AAGAAAGACG CCAAACCAAA ACAGCCATCA ATGGACGATT CAGCGGCTCT GGACATGTTG 
TCTAGTGATT TTTCAGATGT ACCGACTGTA AAGCCCACTG TATCTGATGG CAAAAGCTTC 
ACCCCTGAAC CAACACCACC AACCTTTAAG GCAACCGGTC CGGTTTTAGA CAAACTGGCA 
GACAACGTGC TTTCCAACCT GACTGACCCC AAAGCCAAAG ACAGCAAAGC AAAGGGAAAG 
GGGGCGAAAC CAAAGTCTAA ACCAAAGAAA CAGGCAGTGG AAGACTCTTC AGCCACAGAT 
CTGCTGTCCA GTAAACCGAG CTCAGATGTG GTGCCTTCAG CTTCCACAAA GGCTGGAAAA 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_017352801.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208290.1 Danio rerio calpastatin (cast), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa56797
Clone ID Related Accession (Same CDS sequence)	XM_017352801.1 , XM_017352801.2
Accession Version	XM_017352801.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	calpastatin isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTACG CAATGTATTG GGCGCGCAAC AATGGAGACA CTTCATTTCA AAAACAAGAA 
TACGCTCCAC AGACATCAAG GTTCATGTCT CAGCAGGTCA CACCCACCAC AACATCTCAG 
GTCTCCACAG TGAAACCTGC ACAATATGAG AAAGGAGCTA CACAATCGTC TACAGCAGCT 
GCGACTGTCA AATCCGGCGC TGGAGATCCT GCAACCACCC AGAAAGGATC TTCTCAAGTC 
AAACCTCAGG TCACAGATTC TAAATCAACA CCTGCATCTT CTGCTAAAGT CTCAGCTGTG 
AGCTCTGGGA CTGGAGTTGC TGGTACAACC GGTGGAGCAG GAGTGAAACC AGCAGATCCT 
GCAAAAGATA CGCCTCAGAG CACCACCATT CCTTCAACCA AACTGGGACC TGCTAAGGTG 
GATCCGGCTG CTGGGAAGCC CTCTGGCTCA GCTGGTGCCC AAAAACAGAC AAGTGCTCAA 
AAGGTGCAGG TTGAAGTGGG TCCTTCAGCA GCAAAAGCTG ACGATCCGTT TGATGCCCTG 
TCTGGCACAT TACCATCATC ACAACCTTTG GCTCCTAAAG TCCACTACAC TGGACCTGAG 
ATCAAAGAGC CCAAACTGAA ATCAGAGAAA GGTGTTTTGT GTGGTGAGCG AGATGACACG 
CTGCCGCCCG GATACAGAAA GGAAGACCTG GAAAAGAAGC ATGGAGCTGG AGTTCCAGCT 
GGAGTTCCGG AGAAGCCTAA AGAAGTTCCC AAGCCCATAA GCACAGATGA TGCACTGGAA 
TCTCTCTCCT CTACGTTTGT GTCTCCAGCT CAAGCTGCTC CAAAGAAGAC AGACGTGAAA 
ACTGAAACAA TAGGAGCAGT GGATGTTAAA TCGGCAGGCA CCACTAACTT TGCTCCCCCT 
CCACCATCTC AGCAGAAACA ACCGGCGTCG TCTCAAGCTG CTGCTGTAAC CAAATCACCT 
GCTCCACCGG CAGACAAGAA AGCTAAAATG GAGACTCATG CACAACCAGC CAAAGCAAAG 
ACAGAGTCGG ACAGCTCCAT ATCGCTGGAC GCTCTCGATG CTTTGAGCGA CACACTGGGG 
GCTTCTAAAC CACCCCAAAA ATTACCAGAA CTCAAACCTG GACAGATAGT TGATGAGAAG 
AAACAGACCT CAGAGAAAGG TGTTCGTGTT GGAGAAAGAG ACGACACACT TCCACCACAA 
TACAGATTCA AAGAGGAAGA TCTCAAGAAA TATCCTCCAC CTGCGAAAGA GCCTTCACTG 
AACACAGATG ATGCGATGGA CATACTTTCA GGAGATTTCA CGACCCCTGC ATCGGCACCT 
GTTGTCAAAG CTCCTGTTCC TCCTGCACAG GACACAAAGA AGCATGCTGC AGTGCCTGAG 
AAGACCAAAG ATATTCCCAA GACTAAAGCT GATGCTCTGT CAAGTGATTT CGTGGCTCCG 
GCACATTCTG CTCCTAAGGT TTCTTCAGCT GCTCCTAAAA CCATCCCTCC AGGCCCACCA 
CAAACCCCAG ATGAGGATCC TTTCAGTGCT TTGGGCGACA CTCTTTCTGC CCCAGAACCA 
CCAAAAAAAC AACCTGACCT GAAACCTAAG GACATCGTAC ATGAGAAGGA TGTGACGTCA 
AAGAAAGGAG TTCGTGTTGG AGAAAGAGAC GACACACTCC CACCAGAATA CAGATTCAAA 
GAGGAAGATC TCAAGAAATA TCCTCCTCCT CAGAAAGATC CTTCCTTAGA TGCTAATGAA 
GCTCTGGATA TTCTGTCTGG GGATTTTACA ACTCCTTCCG CACCATCGGC TGTTTCCCCT 
GCCTCTAAAT CCTCTGCCAA GTCTTCAGAT TCAGCTTTAG ATGCTCTTGC TGGTGATTTC 
GTCGCTTCTT CTTCTGCGTC TTCAGTTCAT TCTGCCGTCT CTGGCCCCCC ACGCTCCGAC 
AGACTGTTGT CAGACGGAAC CTCATCAGCT CTAGATGCCC TGTCAGAAAC TCTGGGAGAC 
ATTAAAGCAG CCCCTGAACC CGCCCCTGTC CTACCTAAAA CTGTCGTTAA GGAGAAGACG 
GTCATAGAAG AGAGAGTGAG TAAACCGGGT GAGAGAGACG ACTCCCTTCC ACCAGATCAG 
CGGTTCACTG CGGAGGATCG CAAGGCATTT GAAGAAGCAA AGAAGAAAGA CGCCAAACCA 
AAACAGCCAT CAATGGACGA TTCAGCGGCT CTGGACATGT TGTCTAGTGA TTTTTCAGAT 
GTACCGACTG TAAAGCCCAC TGTATCTGAT GGCAAAAGCT TCACCCCTGA ACCAACACCA 
CCAACCTTTA AGGCAACCGG TCCGGTTTTA GACAAACTGG CAGACAACGT GCTTTCCAAC 
CTGACTGACC CCAAAGCCAA AGACAGCAAA GCAAAGGGAA AGGGGGCGAA ACCAAAGTCT 
AAACCAAAGA AACAGGCAGT GGAAGACTCT TCAGCCACAG ATCTGCTGTC CAGTAAACCG 
AGCTCAGATG TGGTGCCTTC AGCTTCCACA AAGGCTGGAA AAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208290.1
CDS	529..3036
Translation	MAYAMYWARNNGDTSFQKQEYAPQTSRFMSQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQVTDSKSTPASSAKVSAVSSGTGVAGTTGGAGVKPADPAKDTPQSTTIPSTKLGPAKVDPAAGKPSGSAGAQKQTSAQKVQVEVGPSAAKADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_017352801.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352801.1

ORF Insert Sequence:

ATGGCGTACG CAATGTATTG GGCGCGCAAC AATGGAGACA CTTCATTTCA AAAACAAGAA 
TACGCTCCAC AGACATCAAG GTTCATGTCT CAGCAGGTCA CACCCACCAC AACATCTCAG 
GTCTCCACAG TGAAACCTGC ACAATATGAG AAAGGAGCTA CACAATCGTC TACAGCAGCT 
GCGACTGTCA AATCCGGCGC TGGAGATCCT GCAACCACCC AGAAAGGATC TTCTCAAGTC 
AAACCTCAGG TCACAGATTC TAAATCAACA CCTGCATCTT CTGCTAAAGT CTCAGCTGTG 
AGCTCTGGGA CTGGAGTTGC TGGTACAACC GGTGGAGCAG GAGTGAAACC AGCAGATCCT 
GCAAAAGATA CGCCTCAGAG CACCACCATT CCTTCAACCA AACTGGGACC TGCTAAGGTG 
GATCCGGCTG CTGGGAAGCC CTCTGGCTCA GCTGGTGCCC AAAAACAGAC AAGTGCTCAA 
AAGGTGCAGG TTGAAGTGGG TCCTTCAGCA GCAAAAGCTG ACGATCCGTT TGATGCCCTG 
TCTGGCACAT TACCATCATC ACAACCTTTG GCTCCTAAAG TCCACTACAC TGGACCTGAG 
ATCAAAGAGC CCAAACTGAA ATCAGAGAAA GGTGTTTTGT GTGGTGAGCG AGATGACACG 
CTGCCGCCCG GATACAGAAA GGAAGACCTG GAAAAGAAGC ATGGAGCTGG AGTTCCAGCT 
GGAGTTCCGG AGAAGCCTAA AGAAGTTCCC AAGCCCATAA GCACAGATGA TGCACTGGAA 
TCTCTCTCCT CTACGTTTGT GTCTCCAGCT CAAGCTGCTC CAAAGAAGAC AGACGTGAAA 
ACTGAAACAA TAGGAGCAGT GGATGTTAAA TCGGCAGGCA CCACTAACTT TGCTCCCCCT 
CCACCATCTC AGCAGAAACA ACCGGCGTCG TCTCAAGCTG CTGCTGTAAC CAAATCACCT 
GCTCCACCGG CAGACAAGAA AGCTAAAATG GAGACTCATG CACAACCAGC CAAAGCAAAG 
ACAGAGTCGG ACAGCTCCAT ATCGCTGGAC GCTCTCGATG CTTTGAGCGA CACACTGGGG 
GCTTCTAAAC CACCCCAAAA ATTACCAGAA CTCAAACCTG GACAGATAGT TGATGAGAAG 
AAACAGACCT CAGAGAAAGG TGTTCGTGTT GGAGAAAGAG ACGACACACT TCCACCACAA 
TACAGATTCA AAGAGGAAGA TCTCAAGAAA TATCCTCCAC CTGCGAAAGA GCCTTCACTG 
AACACAGATG ATGCGATGGA CATACTTTCA GGAGATTTCA CGACCCCTGC ATCGGCACCT 
GTTGTCAAAG CTCCTGTTCC TCCTGCACAG GACACAAAGA AGCATGCTGC AGTGCCTGAG 
AAGACCAAAG ATATTCCCAA GACTAAAGCT GATGCTCTGT CAAGTGATTT CGTGGCTCCG 
GCACATTCTG CTCCTAAGGT TTCTTCAGCT GCTCCTAAAA CCATCCCTCC AGGCCCACCA 
CAAACCCCAG ATGAGGATCC TTTCAGTGCT TTGGGCGACA CTCTTTCTGC CCCAGAACCA 
CCAAAAAAAC AACCTGACCT GAAACCTAAG GACATCGTAC ATGAGAAGGA TGTGACGTCA 
AAGAAAGGAG TTCGTGTTGG AGAAAGAGAC GACACACTCC CACCAGAATA CAGATTCAAA 
GAGGAAGATC TCAAGAAATA TCCTCCTCCT CAGAAAGATC CTTCCTTAGA TGCTAATGAA 
GCTCTGGATA TTCTGTCTGG GGATTTTACA ACTCCTTCCG CACCATCGGC TGTTTCCCCT 
GCCTCTAAAT CCTCTGCCAA GTCTTCAGAT TCAGCTTTAG ATGCTCTTGC TGGTGATTTC 
GTCGCTTCTT CTTCTGCGTC TTCAGTTCAT TCTGCCGTCT CTGGCCCCCC ACGCTCCGAC 
AGACTGTTGT CAGACGGAAC CTCATCAGCT CTAGATGCCC TGTCAGAAAC TCTGGGAGAC 
ATTAAAGCAG CCCCTGAACC CGCCCCTGTC CTACCTAAAA CTGTCGTTAA GGAGAAGACG 
GTCATAGAAG AGAGAGTGAG TAAACCGGGT GAGAGAGACG ACTCCCTTCC ACCAGATCAG 
CGGTTCACTG CGGAGGATCG CAAGGCATTT GAAGAAGCAA AGAAGAAAGA CGCCAAACCA 
AAACAGCCAT CAATGGACGA TTCAGCGGCT CTGGACATGT TGTCTAGTGA TTTTTCAGAT 
GTACCGACTG TAAAGCCCAC TGTATCTGAT GGCAAAAGCT TCACCCCTGA ACCAACACCA 
CCAACCTTTA AGGCAACCGG TCCGGTTTTA GACAAACTGG CAGACAACGT GCTTTCCAAC 
CTGACTGACC CCAAAGCCAA AGACAGCAAA GCAAAGGGAA AGGGGGCGAA ACCAAAGTCT 
AAACCAAAGA AACAGGCAGT GGAAGACTCT TCAGCCACAG ATCTGCTGTC CAGTAAACCG 
AGCTCAGATG TGGTGCCTTC AGCTTCCACA AAGGCTGGAA AAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_017352801.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208290.1 Danio rerio calpastatin (cast), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa56797
Clone ID Related Accession (Same CDS sequence)	XM_017352801.1 , XM_017352801.2
Accession Version	XM_017352801.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calpastatin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352801.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTACG CAATGTATTG GGCGCGCAAC AATGGAGACA CTTCATTTCA AAAACAAGAA 
TACGCTCCAC AGACATCAAG GTTCATGTCT CAGCAGGTCA CACCCACCAC AACATCTCAG 
GTCTCCACAG TGAAACCTGC ACAATATGAG AAAGGAGCTA CACAATCGTC TACAGCAGCT 
GCGACTGTCA AATCCGGCGC TGGAGATCCT GCAACCACCC AGAAAGGATC TTCTCAAGTC 
AAACCTCAGG TCACAGATTC TAAATCAACA CCTGCATCTT CTGCTAAAGT CTCAGCTGTG 
AGCTCTGGGA CTGGAGTTGC TGGTACAACC GGTGGAGCAG GAGTGAAACC AGCAGATCCT 
GCAAAAGATA CGCCTCAGAG CACCACCATT CCTTCAACCA AACTGGGACC TGCTAAGGTG 
GATCCGGCTG CTGGGAAGCC CTCTGGCTCA GCTGGTGCCC AAAAACAGAC AAGTGCTCAA 
AAGGTGCAGG TTGAAGTGGG TCCTTCAGCA GCAAAAGCTG ACGATCCGTT TGATGCCCTG 
TCTGGCACAT TACCATCATC ACAACCTTTG GCTCCTAAAG TCCACTACAC TGGACCTGAG 
ATCAAAGAGC CCAAACTGAA ATCAGAGAAA GGTGTTTTGT GTGGTGAGCG AGATGACACG 
CTGCCGCCCG GATACAGAAA GGAAGACCTG GAAAAGAAGC ATGGAGCTGG AGTTCCAGCT 
GGAGTTCCGG AGAAGCCTAA AGAAGTTCCC AAGCCCATAA GCACAGATGA TGCACTGGAA 
TCTCTCTCCT CTACGTTTGT GTCTCCAGCT CAAGCTGCTC CAAAGAAGAC AGACGTGAAA 
ACTGAAACAA TAGGAGCAGT GGATGTTAAA TCGGCAGGCA CCACTAACTT TGCTCCCCCT 
CCACCATCTC AGCAGAAACA ACCGGCGTCG TCTCAAGCTG CTGCTGTAAC CAAATCACCT 
GCTCCACCGG CAGACAAGAA AGCTAAAATG GAGACTCATG CACAACCAGC CAAAGCAAAG 
ACAGAGTCGG ACAGCTCCAT ATCGCTGGAC GCTCTCGATG CTTTGAGCGA CACACTGGGG 
GCTTCTAAAC CACCCCAAAA ATTACCAGAA CTCAAACCTG GACAGATAGT TGATGAGAAG 
AAACAGACCT CAGAGAAAGG TGTTCGTGTT GGAGAAAGAG ACGACACACT TCCACCACAA 
TACAGATTCA AAGAGGAAGA TCTCAAGAAA TATCCTCCAC CTGCGAAAGA GCCTTCACTG 
AACACAGATG ATGCGATGGA CATACTTTCA GGAGATTTCA CGACCCCTGC ATCGGCACCT 
GTTGTCAAAG CTCCTGTTCC TCCTGCACAG GACACAAAGA AGCATGCTGC AGTGCCTGAG 
AAGACCAAAG ATATTCCCAA GACTAAAGCT GATGCTCTGT CAAGTGATTT CGTGGCTCCG 
GCACATTCTG CTCCTAAGGT TTCTTCAGCT GCTCCTAAAA CCATCCCTCC AGGCCCACCA 
CAAACCCCAG ATGAGGATCC TTTCAGTGCT TTGGGCGACA CTCTTTCTGC CCCAGAACCA 
CCAAAAAAAC AACCTGACCT GAAACCTAAG GACATCGTAC ATGAGAAGGA TGTGACGTCA 
AAGAAAGGAG TTCGTGTTGG AGAAAGAGAC GACACACTCC CACCAGAATA CAGATTCAAA 
GAGGAAGATC TCAAGAAATA TCCTCCTCCT CAGAAAGATC CTTCCTTAGA TGCTAATGAA 
GCTCTGGATA TTCTGTCTGG GGATTTTACA ACTCCTTCCG CACCATCGGC TGTTTCCCCT 
GCCTCTAAAT CCTCTGCCAA GTCTTCAGAT TCAGCTTTAG ATGCTCTTGC TGGTGATTTC 
GTCGCTTCTT CTTCTGCGTC TTCAGTTCAT TCTGCCGTCT CTGGCCCCCC ACGCTCCGAC 
AGACTGTTGT CAGACGGAAC CTCATCAGCT CTAGATGCCC TGTCAGAAAC TCTGGGAGAC 
ATTAAAGCAG CCCCTGAACC CGCCCCTGTC CTACCTAAAA CTGTCGTTAA GGAGAAGACG 
GTCATAGAAG AGAGAGTGAG TAAACCGGGT GAGAGAGACG ACTCCCTTCC ACCAGATCAG 
CGGTTCACTG CGGAGGATCG CAAGGCATTT GAAGAAGCAA AGAAGAAAGA CGCCAAACCA 
AAACAGCCAT CAATGGACGA TTCAGCGGCT CTGGACATGT TGTCTAGTGA TTTTTCAGAT 
GTACCGACTG TAAAGCCCAC TGTATCTGAT GGCAAAAGCT TCACCCCTGA ACCAACACCA 
CCAACCTTTA AGGCAACCGG TCCGGTTTTA GACAAACTGG CAGACAACGT GCTTTCCAAC 
CTGACTGACC CCAAAGCCAA AGACAGCAAA GCAAAGGGAA AGGGGGCGAA ACCAAAGTCT 
AAACCAAAGA AACAGGCAGT GGAAGACTCT TCAGCCACAG ATCTGCTGTC CAGTAAACCG 
AGCTCAGATG TGGTGCCTTC AGCTTCCACA AAGGCTGGAA AAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208290.1
CDS	113..2620
Translation	MAYAMYWARNNGDTSFQKQEYAPQTSRFMSQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQVTDSKSTPASSAKVSAVSSGTGVAGTTGGAGVKPADPAKDTPQSTTIPSTKLGPAKVDPAAGKPSGSAGAQKQTSAQKVQVEVGPSAAKADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_017352801.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352801.2

ORF Insert Sequence:

ATGGCGTACG CAATGTATTG GGCGCGCAAC AATGGAGACA CTTCATTTCA AAAACAAGAA 
TACGCTCCAC AGACATCAAG GTTCATGTCT CAGCAGGTCA CACCCACCAC AACATCTCAG 
GTCTCCACAG TGAAACCTGC ACAATATGAG AAAGGAGCTA CACAATCGTC TACAGCAGCT 
GCGACTGTCA AATCCGGCGC TGGAGATCCT GCAACCACCC AGAAAGGATC TTCTCAAGTC 
AAACCTCAGG TCACAGATTC TAAATCAACA CCTGCATCTT CTGCTAAAGT CTCAGCTGTG 
AGCTCTGGGA CTGGAGTTGC TGGTACAACC GGTGGAGCAG GAGTGAAACC AGCAGATCCT 
GCAAAAGATA CGCCTCAGAG CACCACCATT CCTTCAACCA AACTGGGACC TGCTAAGGTG 
GATCCGGCTG CTGGGAAGCC CTCTGGCTCA GCTGGTGCCC AAAAACAGAC AAGTGCTCAA 
AAGGTGCAGG TTGAAGTGGG TCCTTCAGCA GCAAAAGCTG ACGATCCGTT TGATGCCCTG 
TCTGGCACAT TACCATCATC ACAACCTTTG GCTCCTAAAG TCCACTACAC TGGACCTGAG 
ATCAAAGAGC CCAAACTGAA ATCAGAGAAA GGTGTTTTGT GTGGTGAGCG AGATGACACG 
CTGCCGCCCG GATACAGAAA GGAAGACCTG GAAAAGAAGC ATGGAGCTGG AGTTCCAGCT 
GGAGTTCCGG AGAAGCCTAA AGAAGTTCCC AAGCCCATAA GCACAGATGA TGCACTGGAA 
TCTCTCTCCT CTACGTTTGT GTCTCCAGCT CAAGCTGCTC CAAAGAAGAC AGACGTGAAA 
ACTGAAACAA TAGGAGCAGT GGATGTTAAA TCGGCAGGCA CCACTAACTT TGCTCCCCCT 
CCACCATCTC AGCAGAAACA ACCGGCGTCG TCTCAAGCTG CTGCTGTAAC CAAATCACCT 
GCTCCACCGG CAGACAAGAA AGCTAAAATG GAGACTCATG CACAACCAGC CAAAGCAAAG 
ACAGAGTCGG ACAGCTCCAT ATCGCTGGAC GCTCTCGATG CTTTGAGCGA CACACTGGGG 
GCTTCTAAAC CACCCCAAAA ATTACCAGAA CTCAAACCTG GACAGATAGT TGATGAGAAG 
AAACAGACCT CAGAGAAAGG TGTTCGTGTT GGAGAAAGAG ACGACACACT TCCACCACAA 
TACAGATTCA AAGAGGAAGA TCTCAAGAAA TATCCTCCAC CTGCGAAAGA GCCTTCACTG 
AACACAGATG ATGCGATGGA CATACTTTCA GGAGATTTCA CGACCCCTGC ATCGGCACCT 
GTTGTCAAAG CTCCTGTTCC TCCTGCACAG GACACAAAGA AGCATGCTGC AGTGCCTGAG 
AAGACCAAAG ATATTCCCAA GACTAAAGCT GATGCTCTGT CAAGTGATTT CGTGGCTCCG 
GCACATTCTG CTCCTAAGGT TTCTTCAGCT GCTCCTAAAA CCATCCCTCC AGGCCCACCA 
CAAACCCCAG ATGAGGATCC TTTCAGTGCT TTGGGCGACA CTCTTTCTGC CCCAGAACCA 
CCAAAAAAAC AACCTGACCT GAAACCTAAG GACATCGTAC ATGAGAAGGA TGTGACGTCA 
AAGAAAGGAG TTCGTGTTGG AGAAAGAGAC GACACACTCC CACCAGAATA CAGATTCAAA 
GAGGAAGATC TCAAGAAATA TCCTCCTCCT CAGAAAGATC CTTCCTTAGA TGCTAATGAA 
GCTCTGGATA TTCTGTCTGG GGATTTTACA ACTCCTTCCG CACCATCGGC TGTTTCCCCT 
GCCTCTAAAT CCTCTGCCAA GTCTTCAGAT TCAGCTTTAG ATGCTCTTGC TGGTGATTTC 
GTCGCTTCTT CTTCTGCGTC TTCAGTTCAT TCTGCCGTCT CTGGCCCCCC ACGCTCCGAC 
AGACTGTTGT CAGACGGAAC CTCATCAGCT CTAGATGCCC TGTCAGAAAC TCTGGGAGAC 
ATTAAAGCAG CCCCTGAACC CGCCCCTGTC CTACCTAAAA CTGTCGTTAA GGAGAAGACG 
GTCATAGAAG AGAGAGTGAG TAAACCGGGT GAGAGAGACG ACTCCCTTCC ACCAGATCAG 
CGGTTCACTG CGGAGGATCG CAAGGCATTT GAAGAAGCAA AGAAGAAAGA CGCCAAACCA 
AAACAGCCAT CAATGGACGA TTCAGCGGCT CTGGACATGT TGTCTAGTGA TTTTTCAGAT 
GTACCGACTG TAAAGCCCAC TGTATCTGAT GGCAAAAGCT TCACCCCTGA ACCAACACCA 
CCAACCTTTA AGGCAACCGG TCCGGTTTTA GACAAACTGG CAGACAACGT GCTTTCCAAC 
CTGACTGACC CCAAAGCCAA AGACAGCAAA GCAAAGGGAA AGGGGGCGAA ACCAAAGTCT 
AAACCAAAGA AACAGGCAGT GGAAGACTCT TCAGCCACAG ATCTGCTGTC CAGTAAACCG 
AGCTCAGATG TGGTGCCTTC AGCTTCCACA AAGGCTGGAA AAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_017352802.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208291.1 Danio rerio calpastatin (cast), transcript variant X8, mRNA.

Price & Availability↑

CloneID	ODa56798
Clone ID Related Accession (Same CDS sequence)	XM_017352802.2 , XM_017352802.1
Accession Version	XM_017352802.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calpastatin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352802.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGTCTCA GCAGGTCACA CCCACCACAA CATCTCAGGT CTCCACAGTG 
AAACCTGCAC AATATGAGAA AGGAGCTACA CAATCGTCTA CAGCAGCTGC GACTGTCAAA 
TCCGGCGCTG GAGATCCTGC AACCACCCAG AAAGGATCTT CTCAAGTCAA ACCTCAGGTC 
ACAGATTCTA AATCAACACC TGCATCTTCT GCTAAAGTCT CAGCTGTGAG CTCTGGGACT 
GGAGTTGCTG GTACAACCGG TGGAGCAGGA GTGAAACCAG CAGATCCTGC AAAAGATACG 
CCTCAGAGCA CCACCATTCC TTCAACCAAA CTGGGACCTG CTAAGGTGGA TCCGGCTGCT 
GGGAAGCCCT CTGGCTCAGC TGGTGCCCAA AAACAGACAA GTGCTCAAAA GGTGCAGGTT 
GAAGTGGGTC CTTCAGCAGC AAAAGCTGAC GATCCGTTTG ATGCCCTGTC TGGCACATTA 
CCATCATCAC AACCTTTGGC TCCTAAAGTC CACTACACTG GACCTGAGAT CAAAGAGCCC 
AAACTGAAAT CAGAGAAAGG TGTTTTGTGT GGTGAGCGAG ATGACACGCT GCCGCCCGGA 
TACAGAAAGG AAGACCTGGA AAAGAAGCAT GGAGCTGGAG TTCCAGCTGG AGTTCCGGAG 
AAGCCTAAAG AAGTTCCCAA GCCCATAAGC ACAGATGATG CACTGGAATC TCTCTCCTCT 
ACGTTTGTGT CTCCAGCTCA AGCTGCTCCA AAGAAGACAG ACGTGAAAAC TGAAACAATA 
GGAGCAGTGG ATGTTAAATC GGCAGGCACC ACTAACTTTG CTCCCCCTCC ACCATCTCAG 
CAGAAACAAC CGGCGTCGTC TCAAGCTGCT GCTGTAACCA AATCACCTGC TCCACCGGCA 
GACAAGAAAG CTAAAATGGA GACTCATGCA CAACCAGCCA AAGCAAAGAC AGAGTCGGAC 
AGCTCCATAT CGCTGGACGC TCTCGATGCT TTGAGCGACA CACTGGGGGC TTCTAAACCA 
CCCCAAAAAT TACCAGAACT CAAACCTGGA CAGATAGTTG ATGAGAAGAA ACAGACCTCA 
GAGAAAGGTG TTCGTGTTGG AGAAAGAGAC GACACACTTC CACCACAATA CAGATTCAAA 
GAGGAAGATC TCAAGAAATA TCCTCCACCT GCGAAAGAGC CTTCACTGAA CACAGATGAT 
GCGATGGACA TACTTTCAGG AGATTTCACG ACCCCTGCAT CGGCACCTGT TGTCAAAGCT 
CCTGTTCCTC CTGCACAGGA CACAAAGAAG CATGCTGCAG TGCCTGAGAA GACCAAAGAT 
ATTCCCAAGA CTAAAGCTGA TGCTCTGTCA AGTGATTTCG TGGCTCCGGC ACATTCTGCT 
CCTAAGGTTT CTTCAGCTGC TCCTAAAACC ATCCCTCCAG GCCCACCACA AACCCCAGAT 
GAGGATCCTT TCAGTGCTTT GGGCGACACT CTTTCTGCCC CAGAACCACC AAAAAAACAA 
CCTGACCTGA AACCTAAGGA CATCGTACAT GAGAAGGATG TGACGTCAAA GAAAGGAGTT 
CGTGTTGGAG AAAGAGACGA CACACTCCCA CCAGAATACA GATTCAAAGA GGAAGATCTC 
AAGAAATATC CTCCTCCTCA GAAAGATCCT TCCTTAGATG CTAATGAAGC TCTGGATATT 
CTGTCTGGGG ATTTTACAAC TCCTTCCGCA CCATCGGCTG TTTCCCCTGC CTCTAAATCC 
TCTGCCAAGT CTTCAGATTC AGCTTTAGAT GCTCTTGCTG GTGATTTCGT CGCTTCTTCT 
TCTGCGTCTT CAGTTCATTC TGCCGTCTCT GGCCCCCCAC GCTCCGACAG ACTGTTGTCA 
GACGGAACCT CATCAGCTCT AGATGCCCTG TCAGAAACTC TGGGAGACAT TAAAGCAGCC 
CCTGAACCCG CCCCTGTCCT ACCTAAAACT GTCGTTAAGG AGAAGACGGT CATAGAAGAG 
AGAGTGAGTA AACCGGGTGA GAGAGACGAC TCCCTTCCAC CAGATCAGCG GTTCACTGCG 
GAGGATCGCA AGGCATTTGA AGAAGCAAAG AAGAAAGACG CCAAACCAAA ACAGCCATCA 
ATGGACGATT CAGCGGCTCT GGACATGTTG TCTAGTGATT TTTCAGATGT ACCGACTGTA 
AAGCCCACTG TATCTGATGG CAAAAGCTTC ACCCCTGAAC CAACACCACC AACCTTTAAG 
GCAACCGGTC CGGTTTTAGA CAAACTGGCA GACAACGTGC TTTCCAACCT GACTGACCCC 
AAAGCCAAAG ACAGCAAAGC AAAGGGAAAG GGGGCGAAAC CAAAGTCTAA ACCAAAGAAA 
CAGGCAGTGG AAGACTCTTC AGCCACAGAT CTGCTGTCCA GTAAACCGAG CTCAGATGTG 
GTGCCTTCAG CTTCCACAAA GGCTGGAAAA AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208291.1
CDS	72..2567
Translation	MGQIFSWIRGTRDQPALQDIAVEQQSQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQVTDSKSTPASSAKVSAVSSGTGVAGTTGGAGVKPADPAKDTPQSTTIPSTKLGPAKVDPAAGKPSGSAGAQKQTSAQKVQVEVGPSAAKADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_017352802.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352802.2

ORF Insert Sequence:

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGTCTCA GCAGGTCACA CCCACCACAA CATCTCAGGT CTCCACAGTG 
AAACCTGCAC AATATGAGAA AGGAGCTACA CAATCGTCTA CAGCAGCTGC GACTGTCAAA 
TCCGGCGCTG GAGATCCTGC AACCACCCAG AAAGGATCTT CTCAAGTCAA ACCTCAGGTC 
ACAGATTCTA AATCAACACC TGCATCTTCT GCTAAAGTCT CAGCTGTGAG CTCTGGGACT 
GGAGTTGCTG GTACAACCGG TGGAGCAGGA GTGAAACCAG CAGATCCTGC AAAAGATACG 
CCTCAGAGCA CCACCATTCC TTCAACCAAA CTGGGACCTG CTAAGGTGGA TCCGGCTGCT 
GGGAAGCCCT CTGGCTCAGC TGGTGCCCAA AAACAGACAA GTGCTCAAAA GGTGCAGGTT 
GAAGTGGGTC CTTCAGCAGC AAAAGCTGAC GATCCGTTTG ATGCCCTGTC TGGCACATTA 
CCATCATCAC AACCTTTGGC TCCTAAAGTC CACTACACTG GACCTGAGAT CAAAGAGCCC 
AAACTGAAAT CAGAGAAAGG TGTTTTGTGT GGTGAGCGAG ATGACACGCT GCCGCCCGGA 
TACAGAAAGG AAGACCTGGA AAAGAAGCAT GGAGCTGGAG TTCCAGCTGG AGTTCCGGAG 
AAGCCTAAAG AAGTTCCCAA GCCCATAAGC ACAGATGATG CACTGGAATC TCTCTCCTCT 
ACGTTTGTGT CTCCAGCTCA AGCTGCTCCA AAGAAGACAG ACGTGAAAAC TGAAACAATA 
GGAGCAGTGG ATGTTAAATC GGCAGGCACC ACTAACTTTG CTCCCCCTCC ACCATCTCAG 
CAGAAACAAC CGGCGTCGTC TCAAGCTGCT GCTGTAACCA AATCACCTGC TCCACCGGCA 
GACAAGAAAG CTAAAATGGA GACTCATGCA CAACCAGCCA AAGCAAAGAC AGAGTCGGAC 
AGCTCCATAT CGCTGGACGC TCTCGATGCT TTGAGCGACA CACTGGGGGC TTCTAAACCA 
CCCCAAAAAT TACCAGAACT CAAACCTGGA CAGATAGTTG ATGAGAAGAA ACAGACCTCA 
GAGAAAGGTG TTCGTGTTGG AGAAAGAGAC GACACACTTC CACCACAATA CAGATTCAAA 
GAGGAAGATC TCAAGAAATA TCCTCCACCT GCGAAAGAGC CTTCACTGAA CACAGATGAT 
GCGATGGACA TACTTTCAGG AGATTTCACG ACCCCTGCAT CGGCACCTGT TGTCAAAGCT 
CCTGTTCCTC CTGCACAGGA CACAAAGAAG CATGCTGCAG TGCCTGAGAA GACCAAAGAT 
ATTCCCAAGA CTAAAGCTGA TGCTCTGTCA AGTGATTTCG TGGCTCCGGC ACATTCTGCT 
CCTAAGGTTT CTTCAGCTGC TCCTAAAACC ATCCCTCCAG GCCCACCACA AACCCCAGAT 
GAGGATCCTT TCAGTGCTTT GGGCGACACT CTTTCTGCCC CAGAACCACC AAAAAAACAA 
CCTGACCTGA AACCTAAGGA CATCGTACAT GAGAAGGATG TGACGTCAAA GAAAGGAGTT 
CGTGTTGGAG AAAGAGACGA CACACTCCCA CCAGAATACA GATTCAAAGA GGAAGATCTC 
AAGAAATATC CTCCTCCTCA GAAAGATCCT TCCTTAGATG CTAATGAAGC TCTGGATATT 
CTGTCTGGGG ATTTTACAAC TCCTTCCGCA CCATCGGCTG TTTCCCCTGC CTCTAAATCC 
TCTGCCAAGT CTTCAGATTC AGCTTTAGAT GCTCTTGCTG GTGATTTCGT CGCTTCTTCT 
TCTGCGTCTT CAGTTCATTC TGCCGTCTCT GGCCCCCCAC GCTCCGACAG ACTGTTGTCA 
GACGGAACCT CATCAGCTCT AGATGCCCTG TCAGAAACTC TGGGAGACAT TAAAGCAGCC 
CCTGAACCCG CCCCTGTCCT ACCTAAAACT GTCGTTAAGG AGAAGACGGT CATAGAAGAG 
AGAGTGAGTA AACCGGGTGA GAGAGACGAC TCCCTTCCAC CAGATCAGCG GTTCACTGCG 
GAGGATCGCA AGGCATTTGA AGAAGCAAAG AAGAAAGACG CCAAACCAAA ACAGCCATCA 
ATGGACGATT CAGCGGCTCT GGACATGTTG TCTAGTGATT TTTCAGATGT ACCGACTGTA 
AAGCCCACTG TATCTGATGG CAAAAGCTTC ACCCCTGAAC CAACACCACC AACCTTTAAG 
GCAACCGGTC CGGTTTTAGA CAAACTGGCA GACAACGTGC TTTCCAACCT GACTGACCCC 
AAAGCCAAAG ACAGCAAAGC AAAGGGAAAG GGGGCGAAAC CAAAGTCTAA ACCAAAGAAA 
CAGGCAGTGG AAGACTCTTC AGCCACAGAT CTGCTGTCCA GTAAACCGAG CTCAGATGTG 
GTGCCTTCAG CTTCCACAAA GGCTGGAAAA AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_017352802.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208291.1 Danio rerio calpastatin (cast), transcript variant X8, mRNA.

Price & Availability↑

CloneID	ODa56798
Clone ID Related Accession (Same CDS sequence)	XM_017352802.2 , XM_017352802.1
Accession Version	XM_017352802.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	calpastatin isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGTCTCA GCAGGTCACA CCCACCACAA CATCTCAGGT CTCCACAGTG 
AAACCTGCAC AATATGAGAA AGGAGCTACA CAATCGTCTA CAGCAGCTGC GACTGTCAAA 
TCCGGCGCTG GAGATCCTGC AACCACCCAG AAAGGATCTT CTCAAGTCAA ACCTCAGGTC 
ACAGATTCTA AATCAACACC TGCATCTTCT GCTAAAGTCT CAGCTGTGAG CTCTGGGACT 
GGAGTTGCTG GTACAACCGG TGGAGCAGGA GTGAAACCAG CAGATCCTGC AAAAGATACG 
CCTCAGAGCA CCACCATTCC TTCAACCAAA CTGGGACCTG CTAAGGTGGA TCCGGCTGCT 
GGGAAGCCCT CTGGCTCAGC TGGTGCCCAA AAACAGACAA GTGCTCAAAA GGTGCAGGTT 
GAAGTGGGTC CTTCAGCAGC AAAAGCTGAC GATCCGTTTG ATGCCCTGTC TGGCACATTA 
CCATCATCAC AACCTTTGGC TCCTAAAGTC CACTACACTG GACCTGAGAT CAAAGAGCCC 
AAACTGAAAT CAGAGAAAGG TGTTTTGTGT GGTGAGCGAG ATGACACGCT GCCGCCCGGA 
TACAGAAAGG AAGACCTGGA AAAGAAGCAT GGAGCTGGAG TTCCAGCTGG AGTTCCGGAG 
AAGCCTAAAG AAGTTCCCAA GCCCATAAGC ACAGATGATG CACTGGAATC TCTCTCCTCT 
ACGTTTGTGT CTCCAGCTCA AGCTGCTCCA AAGAAGACAG ACGTGAAAAC TGAAACAATA 
GGAGCAGTGG ATGTTAAATC GGCAGGCACC ACTAACTTTG CTCCCCCTCC ACCATCTCAG 
CAGAAACAAC CGGCGTCGTC TCAAGCTGCT GCTGTAACCA AATCACCTGC TCCACCGGCA 
GACAAGAAAG CTAAAATGGA GACTCATGCA CAACCAGCCA AAGCAAAGAC AGAGTCGGAC 
AGCTCCATAT CGCTGGACGC TCTCGATGCT TTGAGCGACA CACTGGGGGC TTCTAAACCA 
CCCCAAAAAT TACCAGAACT CAAACCTGGA CAGATAGTTG ATGAGAAGAA ACAGACCTCA 
GAGAAAGGTG TTCGTGTTGG AGAAAGAGAC GACACACTTC CACCACAATA CAGATTCAAA 
GAGGAAGATC TCAAGAAATA TCCTCCACCT GCGAAAGAGC CTTCACTGAA CACAGATGAT 
GCGATGGACA TACTTTCAGG AGATTTCACG ACCCCTGCAT CGGCACCTGT TGTCAAAGCT 
CCTGTTCCTC CTGCACAGGA CACAAAGAAG CATGCTGCAG TGCCTGAGAA GACCAAAGAT 
ATTCCCAAGA CTAAAGCTGA TGCTCTGTCA AGTGATTTCG TGGCTCCGGC ACATTCTGCT 
CCTAAGGTTT CTTCAGCTGC TCCTAAAACC ATCCCTCCAG GCCCACCACA AACCCCAGAT 
GAGGATCCTT TCAGTGCTTT GGGCGACACT CTTTCTGCCC CAGAACCACC AAAAAAACAA 
CCTGACCTGA AACCTAAGGA CATCGTACAT GAGAAGGATG TGACGTCAAA GAAAGGAGTT 
CGTGTTGGAG AAAGAGACGA CACACTCCCA CCAGAATACA GATTCAAAGA GGAAGATCTC 
AAGAAATATC CTCCTCCTCA GAAAGATCCT TCCTTAGATG CTAATGAAGC TCTGGATATT 
CTGTCTGGGG ATTTTACAAC TCCTTCCGCA CCATCGGCTG TTTCCCCTGC CTCTAAATCC 
TCTGCCAAGT CTTCAGATTC AGCTTTAGAT GCTCTTGCTG GTGATTTCGT CGCTTCTTCT 
TCTGCGTCTT CAGTTCATTC TGCCGTCTCT GGCCCCCCAC GCTCCGACAG ACTGTTGTCA 
GACGGAACCT CATCAGCTCT AGATGCCCTG TCAGAAACTC TGGGAGACAT TAAAGCAGCC 
CCTGAACCCG CCCCTGTCCT ACCTAAAACT GTCGTTAAGG AGAAGACGGT CATAGAAGAG 
AGAGTGAGTA AACCGGGTGA GAGAGACGAC TCCCTTCCAC CAGATCAGCG GTTCACTGCG 
GAGGATCGCA AGGCATTTGA AGAAGCAAAG AAGAAAGACG CCAAACCAAA ACAGCCATCA 
ATGGACGATT CAGCGGCTCT GGACATGTTG TCTAGTGATT TTTCAGATGT ACCGACTGTA 
AAGCCCACTG TATCTGATGG CAAAAGCTTC ACCCCTGAAC CAACACCACC AACCTTTAAG 
GCAACCGGTC CGGTTTTAGA CAAACTGGCA GACAACGTGC TTTCCAACCT GACTGACCCC 
AAAGCCAAAG ACAGCAAAGC AAAGGGAAAG GGGGCGAAAC CAAAGTCTAA ACCAAAGAAA 
CAGGCAGTGG AAGACTCTTC AGCCACAGAT CTGCTGTCCA GTAAACCGAG CTCAGATGTG 
GTGCCTTCAG CTTCCACAAA GGCTGGAAAA AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208291.1
CDS	68..2563
Translation	MGQIFSWIRGTRDQPALQDIAVEQQSQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQVTDSKSTPASSAKVSAVSSGTGVAGTTGGAGVKPADPAKDTPQSTTIPSTKLGPAKVDPAAGKPSGSAGAQKQTSAQKVQVEVGPSAAKADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_017352802.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352802.1

ORF Insert Sequence:

ATGGGCCAGA TCTTCAGCTG GATCCGCGGC ACACGCGACC AACCGGCCCT GCAGGACATC 
GCAGTAGAGC AACAGTCTCA GCAGGTCACA CCCACCACAA CATCTCAGGT CTCCACAGTG 
AAACCTGCAC AATATGAGAA AGGAGCTACA CAATCGTCTA CAGCAGCTGC GACTGTCAAA 
TCCGGCGCTG GAGATCCTGC AACCACCCAG AAAGGATCTT CTCAAGTCAA ACCTCAGGTC 
ACAGATTCTA AATCAACACC TGCATCTTCT GCTAAAGTCT CAGCTGTGAG CTCTGGGACT 
GGAGTTGCTG GTACAACCGG TGGAGCAGGA GTGAAACCAG CAGATCCTGC AAAAGATACG 
CCTCAGAGCA CCACCATTCC TTCAACCAAA CTGGGACCTG CTAAGGTGGA TCCGGCTGCT 
GGGAAGCCCT CTGGCTCAGC TGGTGCCCAA AAACAGACAA GTGCTCAAAA GGTGCAGGTT 
GAAGTGGGTC CTTCAGCAGC AAAAGCTGAC GATCCGTTTG ATGCCCTGTC TGGCACATTA 
CCATCATCAC AACCTTTGGC TCCTAAAGTC CACTACACTG GACCTGAGAT CAAAGAGCCC 
AAACTGAAAT CAGAGAAAGG TGTTTTGTGT GGTGAGCGAG ATGACACGCT GCCGCCCGGA 
TACAGAAAGG AAGACCTGGA AAAGAAGCAT GGAGCTGGAG TTCCAGCTGG AGTTCCGGAG 
AAGCCTAAAG AAGTTCCCAA GCCCATAAGC ACAGATGATG CACTGGAATC TCTCTCCTCT 
ACGTTTGTGT CTCCAGCTCA AGCTGCTCCA AAGAAGACAG ACGTGAAAAC TGAAACAATA 
GGAGCAGTGG ATGTTAAATC GGCAGGCACC ACTAACTTTG CTCCCCCTCC ACCATCTCAG 
CAGAAACAAC CGGCGTCGTC TCAAGCTGCT GCTGTAACCA AATCACCTGC TCCACCGGCA 
GACAAGAAAG CTAAAATGGA GACTCATGCA CAACCAGCCA AAGCAAAGAC AGAGTCGGAC 
AGCTCCATAT CGCTGGACGC TCTCGATGCT TTGAGCGACA CACTGGGGGC TTCTAAACCA 
CCCCAAAAAT TACCAGAACT CAAACCTGGA CAGATAGTTG ATGAGAAGAA ACAGACCTCA 
GAGAAAGGTG TTCGTGTTGG AGAAAGAGAC GACACACTTC CACCACAATA CAGATTCAAA 
GAGGAAGATC TCAAGAAATA TCCTCCACCT GCGAAAGAGC CTTCACTGAA CACAGATGAT 
GCGATGGACA TACTTTCAGG AGATTTCACG ACCCCTGCAT CGGCACCTGT TGTCAAAGCT 
CCTGTTCCTC CTGCACAGGA CACAAAGAAG CATGCTGCAG TGCCTGAGAA GACCAAAGAT 
ATTCCCAAGA CTAAAGCTGA TGCTCTGTCA AGTGATTTCG TGGCTCCGGC ACATTCTGCT 
CCTAAGGTTT CTTCAGCTGC TCCTAAAACC ATCCCTCCAG GCCCACCACA AACCCCAGAT 
GAGGATCCTT TCAGTGCTTT GGGCGACACT CTTTCTGCCC CAGAACCACC AAAAAAACAA 
CCTGACCTGA AACCTAAGGA CATCGTACAT GAGAAGGATG TGACGTCAAA GAAAGGAGTT 
CGTGTTGGAG AAAGAGACGA CACACTCCCA CCAGAATACA GATTCAAAGA GGAAGATCTC 
AAGAAATATC CTCCTCCTCA GAAAGATCCT TCCTTAGATG CTAATGAAGC TCTGGATATT 
CTGTCTGGGG ATTTTACAAC TCCTTCCGCA CCATCGGCTG TTTCCCCTGC CTCTAAATCC 
TCTGCCAAGT CTTCAGATTC AGCTTTAGAT GCTCTTGCTG GTGATTTCGT CGCTTCTTCT 
TCTGCGTCTT CAGTTCATTC TGCCGTCTCT GGCCCCCCAC GCTCCGACAG ACTGTTGTCA 
GACGGAACCT CATCAGCTCT AGATGCCCTG TCAGAAACTC TGGGAGACAT TAAAGCAGCC 
CCTGAACCCG CCCCTGTCCT ACCTAAAACT GTCGTTAAGG AGAAGACGGT CATAGAAGAG 
AGAGTGAGTA AACCGGGTGA GAGAGACGAC TCCCTTCCAC CAGATCAGCG GTTCACTGCG 
GAGGATCGCA AGGCATTTGA AGAAGCAAAG AAGAAAGACG CCAAACCAAA ACAGCCATCA 
ATGGACGATT CAGCGGCTCT GGACATGTTG TCTAGTGATT TTTCAGATGT ACCGACTGTA 
AAGCCCACTG TATCTGATGG CAAAAGCTTC ACCCCTGAAC CAACACCACC AACCTTTAAG 
GCAACCGGTC CGGTTTTAGA CAAACTGGCA GACAACGTGC TTTCCAACCT GACTGACCCC 
AAAGCCAAAG ACAGCAAAGC AAAGGGAAAG GGGGCGAAAC CAAAGTCTAA ACCAAAGAAA 
CAGGCAGTGG AAGACTCTTC AGCCACAGAT CTGCTGTCCA GTAAACCGAG CTCAGATGTG 
GTGCCTTCAG CTTCCACAAA GGCTGGAAAA AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_017352803.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208292.1 Danio rerio calpastatin (cast), transcript variant X12, mRNA.

Price & Availability↑

CloneID	ODa56799
Clone ID Related Accession (Same CDS sequence)	XM_017352803.1
Accession Version	XM_017352803.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	calpastatin isoform X12
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCACA AAAAGAGATG TCATGGTCGT AAGAAAAACA AAAAGGAGAA TGGAGACACT 
TCATTTCAAA AACAAGAATA CGCTCCACAG ACATCAAGGT TCATGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGAA AGGAGCTACA 
CAATCGTCTA CAGCAGCTGC GACTGTCAAA TCCGGCGCTG GAGATCCTGC AACCACCCAG 
AAAGGATCTT CTCAAGTCAA ACCTCAGGTC ACAGATTCTA AATCAACACC TGCATCTTCT 
GCTAAAGTCT CAGCTGTGAG CTCTGGGACT GGAGTTGCTG GTACAACCGG TGGAGCAGGA 
GTGAAACCAG CAGATCCTGC AAAAGATACG CCTCAGGTGC AGGTTGAAGT GGGTCCTTCA 
GCAGCAAAAG TCAGTGCAGA TGCTGACGAT CCGTTTGATG CCCTGTCTGG CACATTACCA 
TCATCACAAC CTTTGGCTCC TAAAGTCCAC TACACTGGAC CTGAGATCAA AGAGCCCAAA 
CTGAAATCAG AGAAAGGTGT TTTGTGTGGT GAGCGAGATG ACACGCTGCC GCCCGGATAC 
AGAAAGGAAG ACCTGGAAAA GAAGCATGGA GCTGGAGTTC CAGCTGGAGT TCCGGAGAAG 
CCTAAAGAAG TTCCCAAGCC CATAAGCACA GATGATGCAC TGGAATCTCT CTCCTCTACG 
TTTGTGTCTC CAGCTCAAGC TGCTCCAAAG AAGACAGACG TGAAAACTGA AACAATAGGA 
GCAGTGGATG TTAAATCGGC AGGCACCACT AACTTTGCTC CCCCTCCACC ATCTCAGCAG 
AAACAACCGG CGTCGTCTCA AGCTGCTGCT GTAACCAAAT CACCTGCTCC ACCGGCAGAC 
AAGAAAGCTA AAATGGAGAC TCATGCACAA CCAGCCAAAG CAAAGACAGA GTCGGACAGC 
TCCATATCGC TGGACGCTCT CGATGCTTTG AGCGACACAC TGGGGGCTTC TAAACCACCC 
CAAAAATTAC CAGAACTCAA ACCTGGACAG ATAGTTGATG AGAAGAAACA GACCTCAGAG 
AAAGGTGTTC GTGTTGGAGA AAGAGACGAC ACACTTCCAC CACAATACAG ATTCAAAGAG 
GAAGATCTCA AGAAATATCC TCCACCTGCG AAAGAGCCTT CACTGAACAC AGATGATGCG 
ATGGACATAC TTTCAGGAGA TTTCACGACC CCTGCATCGG CACCTGTTGT CAAAGCTCCT 
GTTCCTCCTG CACAGGACAC AAAGAAGCAT GCTGCAGTGC CTGAGAAGAC CAAAGATATT 
CCCAAGACTA AAGCTGATGC TCTGTCAAGT GATTTCGTGG CTCCGGCACA TTCTGCTCCT 
AAGGTTTCTT CAGCTGCTCC TAAAACCATC CCTCCAGGCC CACCACAAAC CCCAGATGAG 
GATCCTTTCA GTGCTTTGGG CGACACTCTT TCTGCCCCAG AACCACCAAA AAAACAACCT 
GACCTGAAAC CTAAGGACAT CGTACATGAG AAGGATGTGA CGTCAAAGAA AGGAGTTCGT 
GTTGGAGAAA GAGACGACAC ACTCCCACCA GAATACAGAT TCAAAGAGGA AGATCTCAAG 
AAATATCCTC CTCCTCAGAA AGATCCTTCC TTAGATGCTA ATGAAGCTCT GGATATTCTG 
TCTGGGGATT TTACAACTCC TTCCGCACCA TCGGCTGTTT CCCCTGCCTC TAAATCCTCT 
GCCAAGTCTT CAGATTCAGC TTTAGATGCT CTTGCTGGTG ATTTCGTCGC TTCTTCTTCT 
GCGTCTTCAG TTCATTCTGC CGTCTCTGGC CCCCCACGCT CCGACAGACT GTTGTCAGAC 
GGAACCTCAT CAGCTCTAGA TGCCCTGTCA GAAACTCTGG GAGACATTAA AGCAGCCCCT 
GAACCCGCCC CTGTCCTACC TAAAACTGTC GTTAAGGAGA AGACGGTCAT AGAAGAGAGA 
GTGAGTAAAC CGGGTGAGAG AGACGACTCC CTTCCACCAG ATCAGCGGTT CACTGCGGAG 
GATCGCAAGG CATTTGAAGA AGCAAAGAAG AAAGACGCCA AACCAAAACA GCCATCAATG 
GACGATTCAG CGGCTCTGGA CATGTTGTCT AGTGATTTTT CAGATGTACC GACTGTAAAG 
CCCACTGTAT CTGATGGCAA AAGCTTCACC CCTGAACCAA CACCACCAAC CTTTAAGGCA 
ACCGGTCCGG TTTTAGACAA ACTGGCAGAC AACGTGCTTT CCAACCTGAC TGACCCCAAA 
GCCAAAGACA GCAAAGCAAA GGGAAAGGGG GCGAAACCAA AGTCTAAACC AAAGAAACAG 
GCAGTGGAAG ACTCTTCAGC CACAGATCTG CTGTCCAGTA AACCGAGCTC AGATGTGGTG 
CCTTCAGCTT CCACAAAGGC TGGAAAAAGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208292.1
CDS	40..2472
Translation	MPHKKRCHGRKKNKKENGDTSFQKQEYAPQTSRFMSQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQVTDSKSTPASSAKVSAVSSGTGVAGTTGGAGVKPADPAKDTPQVQVEVGPSAAKVSADADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_017352803.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352803.1

ORF Insert Sequence:

ATGCCGCACA AAAAGAGATG TCATGGTCGT AAGAAAAACA AAAAGGAGAA TGGAGACACT 
TCATTTCAAA AACAAGAATA CGCTCCACAG ACATCAAGGT TCATGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGAA AGGAGCTACA 
CAATCGTCTA CAGCAGCTGC GACTGTCAAA TCCGGCGCTG GAGATCCTGC AACCACCCAG 
AAAGGATCTT CTCAAGTCAA ACCTCAGGTC ACAGATTCTA AATCAACACC TGCATCTTCT 
GCTAAAGTCT CAGCTGTGAG CTCTGGGACT GGAGTTGCTG GTACAACCGG TGGAGCAGGA 
GTGAAACCAG CAGATCCTGC AAAAGATACG CCTCAGGTGC AGGTTGAAGT GGGTCCTTCA 
GCAGCAAAAG TCAGTGCAGA TGCTGACGAT CCGTTTGATG CCCTGTCTGG CACATTACCA 
TCATCACAAC CTTTGGCTCC TAAAGTCCAC TACACTGGAC CTGAGATCAA AGAGCCCAAA 
CTGAAATCAG AGAAAGGTGT TTTGTGTGGT GAGCGAGATG ACACGCTGCC GCCCGGATAC 
AGAAAGGAAG ACCTGGAAAA GAAGCATGGA GCTGGAGTTC CAGCTGGAGT TCCGGAGAAG 
CCTAAAGAAG TTCCCAAGCC CATAAGCACA GATGATGCAC TGGAATCTCT CTCCTCTACG 
TTTGTGTCTC CAGCTCAAGC TGCTCCAAAG AAGACAGACG TGAAAACTGA AACAATAGGA 
GCAGTGGATG TTAAATCGGC AGGCACCACT AACTTTGCTC CCCCTCCACC ATCTCAGCAG 
AAACAACCGG CGTCGTCTCA AGCTGCTGCT GTAACCAAAT CACCTGCTCC ACCGGCAGAC 
AAGAAAGCTA AAATGGAGAC TCATGCACAA CCAGCCAAAG CAAAGACAGA GTCGGACAGC 
TCCATATCGC TGGACGCTCT CGATGCTTTG AGCGACACAC TGGGGGCTTC TAAACCACCC 
CAAAAATTAC CAGAACTCAA ACCTGGACAG ATAGTTGATG AGAAGAAACA GACCTCAGAG 
AAAGGTGTTC GTGTTGGAGA AAGAGACGAC ACACTTCCAC CACAATACAG ATTCAAAGAG 
GAAGATCTCA AGAAATATCC TCCACCTGCG AAAGAGCCTT CACTGAACAC AGATGATGCG 
ATGGACATAC TTTCAGGAGA TTTCACGACC CCTGCATCGG CACCTGTTGT CAAAGCTCCT 
GTTCCTCCTG CACAGGACAC AAAGAAGCAT GCTGCAGTGC CTGAGAAGAC CAAAGATATT 
CCCAAGACTA AAGCTGATGC TCTGTCAAGT GATTTCGTGG CTCCGGCACA TTCTGCTCCT 
AAGGTTTCTT CAGCTGCTCC TAAAACCATC CCTCCAGGCC CACCACAAAC CCCAGATGAG 
GATCCTTTCA GTGCTTTGGG CGACACTCTT TCTGCCCCAG AACCACCAAA AAAACAACCT 
GACCTGAAAC CTAAGGACAT CGTACATGAG AAGGATGTGA CGTCAAAGAA AGGAGTTCGT 
GTTGGAGAAA GAGACGACAC ACTCCCACCA GAATACAGAT TCAAAGAGGA AGATCTCAAG 
AAATATCCTC CTCCTCAGAA AGATCCTTCC TTAGATGCTA ATGAAGCTCT GGATATTCTG 
TCTGGGGATT TTACAACTCC TTCCGCACCA TCGGCTGTTT CCCCTGCCTC TAAATCCTCT 
GCCAAGTCTT CAGATTCAGC TTTAGATGCT CTTGCTGGTG ATTTCGTCGC TTCTTCTTCT 
GCGTCTTCAG TTCATTCTGC CGTCTCTGGC CCCCCACGCT CCGACAGACT GTTGTCAGAC 
GGAACCTCAT CAGCTCTAGA TGCCCTGTCA GAAACTCTGG GAGACATTAA AGCAGCCCCT 
GAACCCGCCC CTGTCCTACC TAAAACTGTC GTTAAGGAGA AGACGGTCAT AGAAGAGAGA 
GTGAGTAAAC CGGGTGAGAG AGACGACTCC CTTCCACCAG ATCAGCGGTT CACTGCGGAG 
GATCGCAAGG CATTTGAAGA AGCAAAGAAG AAAGACGCCA AACCAAAACA GCCATCAATG 
GACGATTCAG CGGCTCTGGA CATGTTGTCT AGTGATTTTT CAGATGTACC GACTGTAAAG 
CCCACTGTAT CTGATGGCAA AAGCTTCACC CCTGAACCAA CACCACCAAC CTTTAAGGCA 
ACCGGTCCGG TTTTAGACAA ACTGGCAGAC AACGTGCTTT CCAACCTGAC TGACCCCAAA 
GCCAAAGACA GCAAAGCAAA GGGAAAGGGG GCGAAACCAA AGTCTAAACC AAAGAAACAG 
GCAGTGGAAG ACTCTTCAGC CACAGATCTG CTGTCCAGTA AACCGAGCTC AGATGTGGTG 
CCTTCAGCTT CCACAAAGGC TGGAAAAAGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_017352804.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208293.1 Danio rerio calpastatin (cast), transcript variant X13, mRNA.

Price & Availability↑

CloneID	ODa56800
Clone ID Related Accession (Same CDS sequence)	XM_017352804.1
Accession Version	XM_017352804.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	calpastatin isoform X13
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCACA AAAAGAGATG TCATGGTCGT AAGAAAAACA AAAAGGAGAA TGGAGACACT 
TCATTTCAAA AACAAGAATA CGCTCCACAG ACATCAAGGT TCATGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGAA AGGAGCTACA 
CAATCGTCTA CAGCAGCTGC GACTGTCAAA TCCGGCGCTG GAGATCCTGC AACCACCCAG 
AAAGGATCTT CTCAAGTCAA ACCTCAGGTC ACAGATTCTA AATCAACACC TGCATCTTCT 
GCTAAAGTCT CAGCTGTGAG CTCTGGGACT GGAGTTGCTG GTACAACCGG TGGAGCAGGA 
GTGAAACCAG CAGATCCTGC AAAAGATACG CCTCAGGTGC AGGTTGAAGT GGGTCCTTCA 
GCAGCAAAAG CTGACGATCC GTTTGATGCC CTGTCTGGCA CATTACCATC ATCACAACCT 
TTGGCTCCTA AAGTCCACTA CACTGGACCT GAGATCAAAG AGCCCAAACT GAAATCAGAG 
AAAGGTGTTT TGTGTGGTGA GCGAGATGAC ACGCTGCCGC CCGGATACAG AAAGGAAGAC 
CTGGAAAAGA AGCATGGAGC TGGAGTTCCA GCTGGAGTTC CGGAGAAGCC TAAAGAAGTT 
CCCAAGCCCA TAAGCACAGA TGATGCACTG GAATCTCTCT CCTCTACGTT TGTGTCTCCA 
GCTCAAGCTG CTCCAAAGAA GACAGACGTG AAAACTGAAA CAATAGGAGC AGTGGATGTT 
AAATCGGCAG GCACCACTAA CTTTGCTCCC CCTCCACCAT CTCAGCAGAA ACAACCGGCG 
TCGTCTCAAG CTGCTGCTGT AACCAAATCA CCTGCTCCAC CGGCAGACAA GAAAGCTAAA 
ATGGAGACTC ATGCACAACC AGCCAAAGCA AAGACAGAGT CGGACAGCTC CATATCGCTG 
GACGCTCTCG ATGCTTTGAG CGACACACTG GGGGCTTCTA AACCACCCCA AAAATTACCA 
GAACTCAAAC CTGGACAGAT AGTTGATGAG AAGAAACAGA CCTCAGAGAA AGGTGTTCGT 
GTTGGAGAAA GAGACGACAC ACTTCCACCA CAATACAGAT TCAAAGAGGA AGATCTCAAG 
AAATATCCTC CACCTGCGAA AGAGCCTTCA CTGAACACAG ATGATGCGAT GGACATACTT 
TCAGGAGATT TCACGACCCC TGCATCGGCA CCTGTTGTCA AAGCTCCTGT TCCTCCTGCA 
CAGGACACAA AGAAGCATGC TGCAGTGCCT GAGAAGACCA AAGATATTCC CAAGACTAAA 
GCTGATGCTC TGTCAAGTGA TTTCGTGGCT CCGGCACATT CTGCTCCTAA GGTTTCTTCA 
GCTGCTCCTA AAACCATCCC TCCAGGCCCA CCACAAACCC CAGATGAGGA TCCTTTCAGT 
GCTTTGGGCG ACACTCTTTC TGCCCCAGAA CCACCAAAAA AACAACCTGA CCTGAAACCT 
AAGGACATCG TACATGAGAA GGATGTGACG TCAAAGAAAG GAGTTCGTGT TGGAGAAAGA 
GACGACACAC TCCCACCAGA ATACAGATTC AAAGAGGAAG ATCTCAAGAA ATATCCTCCT 
CCTCAGAAAG ATCCTTCCTT AGATGCTAAT GAAGCTCTGG ATATTCTGTC TGGGGATTTT 
ACAACTCCTT CCGCACCATC GGCTGTTTCC CCTGCCTCTA AATCCTCTGC CAAGTCTTCA 
GATTCAGCTT TAGATGCTCT TGCTGGTGAT TTCGTCGCTT CTTCTTCTGC GTCTTCAGTT 
CATTCTGCCG TCTCTGGCCC CCCACGCTCC GACAGACTGT TGTCAGACGG AACCTCATCA 
GCTCTAGATG CCCTGTCAGA AACTCTGGGA GACATTAAAG CAGCCCCTGA ACCCGCCCCT 
GTCCTACCTA AAACTGTCGT TAAGGAGAAG ACGGTCATAG AAGAGAGAGT GAGTAAACCG 
GGTGAGAGAG ACGACTCCCT TCCACCAGAT CAGCGGTTCA CTGCGGAGGA TCGCAAGGCA 
TTTGAAGAAG CAAAGAAGAA AGACGCCAAA CCAAAACAGC CATCAATGGA CGATTCAGCG 
GCTCTGGACA TGTTGTCTAG TGATTTTTCA GATGTACCGA CTGTAAAGCC CACTGTATCT 
GATGGCAAAA GCTTCACCCC TGAACCAACA CCACCAACCT TTAAGGCAAC CGGTCCGGTT 
TTAGACAAAC TGGCAGACAA CGTGCTTTCC AACCTGACTG ACCCCAAAGC CAAAGACAGC 
AAAGCAAAGG GAAAGGGGGC GAAACCAAAG TCTAAACCAA AGAAACAGGC AGTGGAAGAC 
TCTTCAGCCA CAGATCTGCT GTCCAGTAAA CCGAGCTCAG ATGTGGTGCC TTCAGCTTCC 
ACAAAGGCTG GAAAAAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208293.1
CDS	41..2461
Translation	MPHKKRCHGRKKNKKENGDTSFQKQEYAPQTSRFMSQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQVTDSKSTPASSAKVSAVSSGTGVAGTTGGAGVKPADPAKDTPQVQVEVGPSAAKADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_017352804.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352804.1

ORF Insert Sequence:

ATGCCGCACA AAAAGAGATG TCATGGTCGT AAGAAAAACA AAAAGGAGAA TGGAGACACT 
TCATTTCAAA AACAAGAATA CGCTCCACAG ACATCAAGGT TCATGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGAA AGGAGCTACA 
CAATCGTCTA CAGCAGCTGC GACTGTCAAA TCCGGCGCTG GAGATCCTGC AACCACCCAG 
AAAGGATCTT CTCAAGTCAA ACCTCAGGTC ACAGATTCTA AATCAACACC TGCATCTTCT 
GCTAAAGTCT CAGCTGTGAG CTCTGGGACT GGAGTTGCTG GTACAACCGG TGGAGCAGGA 
GTGAAACCAG CAGATCCTGC AAAAGATACG CCTCAGGTGC AGGTTGAAGT GGGTCCTTCA 
GCAGCAAAAG CTGACGATCC GTTTGATGCC CTGTCTGGCA CATTACCATC ATCACAACCT 
TTGGCTCCTA AAGTCCACTA CACTGGACCT GAGATCAAAG AGCCCAAACT GAAATCAGAG 
AAAGGTGTTT TGTGTGGTGA GCGAGATGAC ACGCTGCCGC CCGGATACAG AAAGGAAGAC 
CTGGAAAAGA AGCATGGAGC TGGAGTTCCA GCTGGAGTTC CGGAGAAGCC TAAAGAAGTT 
CCCAAGCCCA TAAGCACAGA TGATGCACTG GAATCTCTCT CCTCTACGTT TGTGTCTCCA 
GCTCAAGCTG CTCCAAAGAA GACAGACGTG AAAACTGAAA CAATAGGAGC AGTGGATGTT 
AAATCGGCAG GCACCACTAA CTTTGCTCCC CCTCCACCAT CTCAGCAGAA ACAACCGGCG 
TCGTCTCAAG CTGCTGCTGT AACCAAATCA CCTGCTCCAC CGGCAGACAA GAAAGCTAAA 
ATGGAGACTC ATGCACAACC AGCCAAAGCA AAGACAGAGT CGGACAGCTC CATATCGCTG 
GACGCTCTCG ATGCTTTGAG CGACACACTG GGGGCTTCTA AACCACCCCA AAAATTACCA 
GAACTCAAAC CTGGACAGAT AGTTGATGAG AAGAAACAGA CCTCAGAGAA AGGTGTTCGT 
GTTGGAGAAA GAGACGACAC ACTTCCACCA CAATACAGAT TCAAAGAGGA AGATCTCAAG 
AAATATCCTC CACCTGCGAA AGAGCCTTCA CTGAACACAG ATGATGCGAT GGACATACTT 
TCAGGAGATT TCACGACCCC TGCATCGGCA CCTGTTGTCA AAGCTCCTGT TCCTCCTGCA 
CAGGACACAA AGAAGCATGC TGCAGTGCCT GAGAAGACCA AAGATATTCC CAAGACTAAA 
GCTGATGCTC TGTCAAGTGA TTTCGTGGCT CCGGCACATT CTGCTCCTAA GGTTTCTTCA 
GCTGCTCCTA AAACCATCCC TCCAGGCCCA CCACAAACCC CAGATGAGGA TCCTTTCAGT 
GCTTTGGGCG ACACTCTTTC TGCCCCAGAA CCACCAAAAA AACAACCTGA CCTGAAACCT 
AAGGACATCG TACATGAGAA GGATGTGACG TCAAAGAAAG GAGTTCGTGT TGGAGAAAGA 
GACGACACAC TCCCACCAGA ATACAGATTC AAAGAGGAAG ATCTCAAGAA ATATCCTCCT 
CCTCAGAAAG ATCCTTCCTT AGATGCTAAT GAAGCTCTGG ATATTCTGTC TGGGGATTTT 
ACAACTCCTT CCGCACCATC GGCTGTTTCC CCTGCCTCTA AATCCTCTGC CAAGTCTTCA 
GATTCAGCTT TAGATGCTCT TGCTGGTGAT TTCGTCGCTT CTTCTTCTGC GTCTTCAGTT 
CATTCTGCCG TCTCTGGCCC CCCACGCTCC GACAGACTGT TGTCAGACGG AACCTCATCA 
GCTCTAGATG CCCTGTCAGA AACTCTGGGA GACATTAAAG CAGCCCCTGA ACCCGCCCCT 
GTCCTACCTA AAACTGTCGT TAAGGAGAAG ACGGTCATAG AAGAGAGAGT GAGTAAACCG 
GGTGAGAGAG ACGACTCCCT TCCACCAGAT CAGCGGTTCA CTGCGGAGGA TCGCAAGGCA 
TTTGAAGAAG CAAAGAAGAA AGACGCCAAA CCAAAACAGC CATCAATGGA CGATTCAGCG 
GCTCTGGACA TGTTGTCTAG TGATTTTTCA GATGTACCGA CTGTAAAGCC CACTGTATCT 
GATGGCAAAA GCTTCACCCC TGAACCAACA CCACCAACCT TTAAGGCAAC CGGTCCGGTT 
TTAGACAAAC TGGCAGACAA CGTGCTTTCC AACCTGACTG ACCCCAAAGC CAAAGACAGC 
AAAGCAAAGG GAAAGGGGGC GAAACCAAAG TCTAAACCAA AGAAACAGGC AGTGGAAGAC 
TCTTCAGCCA CAGATCTGCT GTCCAGTAAA CCGAGCTCAG ATGTGGTGCC TTCAGCTTCC 
ACAAAGGCTG GAAAAAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_017352805.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208294.1 Danio rerio calpastatin (cast), transcript variant X17, mRNA.

Price & Availability↑

CloneID	ODa56801
Clone ID Related Accession (Same CDS sequence)	XM_017352805.1
Accession Version	XM_017352805.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	calpastatin isoform X17
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCACA AAAAGAGATG TCATGGTCGT AAGAAAAACA AAAAGGAGAA TGGAGACACT 
TCATTTCAAA AACAAGAATA CGCTCCACAG ACATCAAGGT TCATGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGAA AGGAGCTACA 
CAATCGTCTA CAGCAGCTGC GACTGTCAAA TCCGGCGCTG GAGATCCTGC AACCACCCAG 
AAAGGATCTT CTCAAGTCAA ACCTCAGGTG CAGGTTGAAG TGGGTCCTTC AGCAGCAAAA 
GCTGACGATC CGTTTGATGC CCTGTCTGGC ACATTACCAT CATCACAACC TTTGGCTCCT 
AAAGTCCACT ACACTGGACC TGAGATCAAA GAGCCCAAAC TGAAATCAGA GAAAGGTGTT 
TTGTGTGGTG AGCGAGATGA CACGCTGCCG CCCGGATACA GAAAGGAAGA CCTGGAAAAG 
AAGCATGGAG CTGGAGTTCC AGCTGGAGTT CCGGAGAAGC CTAAAGAAGT TCCCAAGCCC 
ATAAGCACAG ATGATGCACT GGAATCTCTC TCCTCTACGT TTGTGTCTCC AGCTCAAGCT 
GCTCCAAAGA AGACAGACGT GAAAACTGAA ACAATAGGAG CAGTGGATGT TAAATCGGCA 
GGCACCACTA ACTTTGCTCC CCCTCCACCA TCTCAGCAGA AACAACCGGC GTCGTCTCAA 
GCTGCTGCTG TAACCAAATC ACCTGCTCCA CCGGCAGACA AGAAAGCTAA AATGGAGACT 
CATGCACAAC CAGCCAAAGC AAAGACAGAG TCGGACAGCT CCATATCGCT GGACGCTCTC 
GATGCTTTGA GCGACACACT GGGGGCTTCT AAACCACCCC AAAAATTACC AGAACTCAAA 
CCTGGACAGA TAGTTGATGA GAAGAAACAG ACCTCAGAGA AAGGTGTTCG TGTTGGAGAA 
AGAGACGACA CACTTCCACC ACAATACAGA TTCAAAGAGG AAGATCTCAA GAAATATCCT 
CCACCTGCGA AAGAGCCTTC ACTGAACACA GATGATGCGA TGGACATACT TTCAGGAGAT 
TTCACGACCC CTGCATCGGC ACCTGTTGTC AAAGCTCCTG TTCCTCCTGC ACAGGACACA 
AAGAAGCATG CTGCAGTGCC TGAGAAGACC AAAGATATTC CCAAGACTAA AGCTGATGCT 
CTGTCAAGTG ATTTCGTGGC TCCGGCACAT TCTGCTCCTA AGGTTTCTTC AGCTGCTCCT 
AAAACCATCC CTCCAGGCCC ACCACAAACC CCAGATGAGG ATCCTTTCAG TGCTTTGGGC 
GACACTCTTT CTGCCCCAGA ACCACCAAAA AAACAACCTG ACCTGAAACC TAAGGACATC 
GTACATGAGA AGGATGTGAC GTCAAAGAAA GGAGTTCGTG TTGGAGAAAG AGACGACACA 
CTCCCACCAG AATACAGATT CAAAGAGGAA GATCTCAAGA AATATCCTCC TCCTCAGAAA 
GATCCTTCCT TAGATGCTAA TGAAGCTCTG GATATTCTGT CTGGGGATTT TACAACTCCT 
TCCGCACCAT CGGCTGTTTC CCCTGCCTCT AAATCCTCTG CCAAGTCTTC AGATTCAGCT 
TTAGATGCTC TTGCTGGTGA TTTCGTCGCT TCTTCTTCTG CGTCTTCAGT TCATTCTGCC 
GTCTCTGGCC CCCCACGCTC CGACAGACTG TTGTCAGACG GAACCTCATC AGCTCTAGAT 
GCCCTGTCAG AAACTCTGGG AGACATTAAA GCAGCCCCTG AACCCGCCCC TGTCCTACCT 
AAAACTGTCG TTAAGGAGAA GACGGTCATA GAAGAGAGAG TGAGTAAACC GGGTGAGAGA 
GACGACTCCC TTCCACCAGA TCAGCGGTTC ACTGCGGAGG ATCGCAAGGC ATTTGAAGAA 
GCAAAGAAGA AAGACGCCAA ACCAAAACAG CCATCAATGG ACGATTCAGC GGCTCTGGAC 
ATGTTGTCTA GTGATTTTTC AGATGTACCG ACTGTAAAGC CCACTGTATC TGATGGCAAA 
AGCTTCACCC CTGAACCAAC ACCACCAACC TTTAAGGCAA CCGGTCCGGT TTTAGACAAA 
CTGGCAGACA ACGTGCTTTC CAACCTGACT GACCCCAAAG CCAAAGACAG CAAAGCAAAG 
GGAAAGGGGG CGAAACCAAA GTCTAAACCA AAGAAACAGG CAGTGGAAGA CTCTTCAGCC 
ACAGATCTGC TGTCCAGTAA ACCGAGCTCA GATGTGGTGC CTTCAGCTTC CACAAAGGCT 
GGAAAAAGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208294.1
CDS	39..2330
Translation	MPHKKRCHGRKKNKKENGDTSFQKQEYAPQTSRFMSQQVTPTTTSQVSTVKPAQYEKGATQSSTAAATVKSGAGDPATTQKGSSQVKPQVQVEVGPSAAKADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_017352805.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352805.1

ORF Insert Sequence:

ATGCCGCACA AAAAGAGATG TCATGGTCGT AAGAAAAACA AAAAGGAGAA TGGAGACACT 
TCATTTCAAA AACAAGAATA CGCTCCACAG ACATCAAGGT TCATGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGAA AGGAGCTACA 
CAATCGTCTA CAGCAGCTGC GACTGTCAAA TCCGGCGCTG GAGATCCTGC AACCACCCAG 
AAAGGATCTT CTCAAGTCAA ACCTCAGGTG CAGGTTGAAG TGGGTCCTTC AGCAGCAAAA 
GCTGACGATC CGTTTGATGC CCTGTCTGGC ACATTACCAT CATCACAACC TTTGGCTCCT 
AAAGTCCACT ACACTGGACC TGAGATCAAA GAGCCCAAAC TGAAATCAGA GAAAGGTGTT 
TTGTGTGGTG AGCGAGATGA CACGCTGCCG CCCGGATACA GAAAGGAAGA CCTGGAAAAG 
AAGCATGGAG CTGGAGTTCC AGCTGGAGTT CCGGAGAAGC CTAAAGAAGT TCCCAAGCCC 
ATAAGCACAG ATGATGCACT GGAATCTCTC TCCTCTACGT TTGTGTCTCC AGCTCAAGCT 
GCTCCAAAGA AGACAGACGT GAAAACTGAA ACAATAGGAG CAGTGGATGT TAAATCGGCA 
GGCACCACTA ACTTTGCTCC CCCTCCACCA TCTCAGCAGA AACAACCGGC GTCGTCTCAA 
GCTGCTGCTG TAACCAAATC ACCTGCTCCA CCGGCAGACA AGAAAGCTAA AATGGAGACT 
CATGCACAAC CAGCCAAAGC AAAGACAGAG TCGGACAGCT CCATATCGCT GGACGCTCTC 
GATGCTTTGA GCGACACACT GGGGGCTTCT AAACCACCCC AAAAATTACC AGAACTCAAA 
CCTGGACAGA TAGTTGATGA GAAGAAACAG ACCTCAGAGA AAGGTGTTCG TGTTGGAGAA 
AGAGACGACA CACTTCCACC ACAATACAGA TTCAAAGAGG AAGATCTCAA GAAATATCCT 
CCACCTGCGA AAGAGCCTTC ACTGAACACA GATGATGCGA TGGACATACT TTCAGGAGAT 
TTCACGACCC CTGCATCGGC ACCTGTTGTC AAAGCTCCTG TTCCTCCTGC ACAGGACACA 
AAGAAGCATG CTGCAGTGCC TGAGAAGACC AAAGATATTC CCAAGACTAA AGCTGATGCT 
CTGTCAAGTG ATTTCGTGGC TCCGGCACAT TCTGCTCCTA AGGTTTCTTC AGCTGCTCCT 
AAAACCATCC CTCCAGGCCC ACCACAAACC CCAGATGAGG ATCCTTTCAG TGCTTTGGGC 
GACACTCTTT CTGCCCCAGA ACCACCAAAA AAACAACCTG ACCTGAAACC TAAGGACATC 
GTACATGAGA AGGATGTGAC GTCAAAGAAA GGAGTTCGTG TTGGAGAAAG AGACGACACA 
CTCCCACCAG AATACAGATT CAAAGAGGAA GATCTCAAGA AATATCCTCC TCCTCAGAAA 
GATCCTTCCT TAGATGCTAA TGAAGCTCTG GATATTCTGT CTGGGGATTT TACAACTCCT 
TCCGCACCAT CGGCTGTTTC CCCTGCCTCT AAATCCTCTG CCAAGTCTTC AGATTCAGCT 
TTAGATGCTC TTGCTGGTGA TTTCGTCGCT TCTTCTTCTG CGTCTTCAGT TCATTCTGCC 
GTCTCTGGCC CCCCACGCTC CGACAGACTG TTGTCAGACG GAACCTCATC AGCTCTAGAT 
GCCCTGTCAG AAACTCTGGG AGACATTAAA GCAGCCCCTG AACCCGCCCC TGTCCTACCT 
AAAACTGTCG TTAAGGAGAA GACGGTCATA GAAGAGAGAG TGAGTAAACC GGGTGAGAGA 
GACGACTCCC TTCCACCAGA TCAGCGGTTC ACTGCGGAGG ATCGCAAGGC ATTTGAAGAA 
GCAAAGAAGA AAGACGCCAA ACCAAAACAG CCATCAATGG ACGATTCAGC GGCTCTGGAC 
ATGTTGTCTA GTGATTTTTC AGATGTACCG ACTGTAAAGC CCACTGTATC TGATGGCAAA 
AGCTTCACCC CTGAACCAAC ACCACCAACC TTTAAGGCAA CCGGTCCGGT TTTAGACAAA 
CTGGCAGACA ACGTGCTTTC CAACCTGACT GACCCCAAAG CCAAAGACAG CAAAGCAAAG 
GGAAAGGGGG CGAAACCAAA GTCTAAACCA AAGAAACAGG CAGTGGAAGA CTCTTCAGCC 
ACAGATCTGC TGTCCAGTAA ACCGAGCTCA GATGTGGTGC CTTCAGCTTC CACAAAGGCT 
GGAAAAAGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_017352806.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208295.1 Danio rerio calpastatin (cast), transcript variant X22, mRNA.

Price & Availability↑

CloneID	ODa56802
Clone ID Related Accession (Same CDS sequence)	XM_017352806.1
Accession Version	XM_017352806.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	calpastatin isoform X22
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCACA AAAAGAGATG TCATGGTCGT AAGAAAAACA AAAAGGAGAA TGGAGACACT 
TCATTTCAAA AACAAGAATA CGCTCCACAG ACATCAAGGT TCATGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGGT GCAGGTTGAA 
GTGGGTCCTT CAGCAGCAAA AGCTGACGAT CCGTTTGATG CCCTGTCTGG CACATTACCA 
TCATCACAAC CTTTGGCTCC TAAAGTCCAC TACACTGGAC CTGAGATCAA AGAGCCCAAA 
CTGAAATCAG AGAAAGGTGT TTTGTGTGGT GAGCGAGATG ACACGCTGCC GCCCGGATAC 
AGAAAGGAAG ACCTGGAAAA GAAGCATGGA GCTGGAGTTC CAGCTGGAGT TCCGGAGAAG 
CCTAAAGAAG TTCCCAAGCC CATAAGCACA GATGATGCAC TGGAATCTCT CTCCTCTACG 
TTTGTGTCTC CAGCTCAAGC TGCTCCAAAG AAGACAGACG TGAAAACTGA AACAATAGGA 
GCAGTGGATG TTAAATCGGC AGGCACCACT AACTTTGCTC CCCCTCCACC ATCTCAGCAG 
AAACAACCGG CGTCGTCTCA AGCTGCTGCT GTAACCAAAT CACCTGCTCC ACCGGCAGAC 
AAGAAAGCTA AAATGGAGAC TCATGCACAA CCAGCCAAAG CAAAGACAGA GTCGGACAGC 
TCCATATCGC TGGACGCTCT CGATGCTTTG AGCGACACAC TGGGGGCTTC TAAACCACCC 
CAAAAATTAC CAGAACTCAA ACCTGGACAG ATAGTTGATG AGAAGAAACA GACCTCAGAG 
AAAGGTGTTC GTGTTGGAGA AAGAGACGAC ACACTTCCAC CACAATACAG ATTCAAAGAG 
GAAGATCTCA AGAAATATCC TCCACCTGCG AAAGAGCCTT CACTGAACAC AGATGATGCG 
ATGGACATAC TTTCAGGAGA TTTCACGACC CCTGCATCGG CACCTGTTGT CAAAGCTCCT 
GTTCCTCCTG CACAGGACAC AAAGAAGCAT GCTGCAGTGC CTGAGAAGAC CAAAGATATT 
CCCAAGACTA AAGCTGATGC TCTGTCAAGT GATTTCGTGG CTCCGGCACA TTCTGCTCCT 
AAGGTTTCTT CAGCTGCTCC TAAAACCATC CCTCCAGGCC CACCACAAAC CCCAGATGAG 
GATCCTTTCA GTGCTTTGGG CGACACTCTT TCTGCCCCAG AACCACCAAA AAAACAACCT 
GACCTGAAAC CTAAGGACAT CGTACATGAG AAGGATGTGA CGTCAAAGAA AGGAGTTCGT 
GTTGGAGAAA GAGACGACAC ACTCCCACCA GAATACAGAT TCAAAGAGGA AGATCTCAAG 
AAATATCCTC CTCCTCAGAA AGATCCTTCC TTAGATGCTA ATGAAGCTCT GGATATTCTG 
TCTGGGGATT TTACAACTCC TTCCGCACCA TCGGCTGTTT CCCCTGCCTC TAAATCCTCT 
GCCAAGTCTT CAGATTCAGC TTTAGATGCT CTTGCTGGTG ATTTCGTCGC TTCTTCTTCT 
GCGTCTTCAG TTCATTCTGC CGTCTCTGGC CCCCCACGCT CCGACAGACT GTTGTCAGAC 
GGAACCTCAT CAGCTCTAGA TGCCCTGTCA GAAACTCTGG GAGACATTAA AGCAGCCCCT 
GAACCCGCCC CTGTCCTACC TAAAACTGTC GTTAAGGAGA AGACGGTCAT AGAAGAGAGA 
GTGAGTAAAC CGGGTGAGAG AGACGACTCC CTTCCACCAG ATCAGCGGTT CACTGCGGAG 
GATCGCAAGG CATTTGAAGA AGCAAAGAAG AAAGACGCCA AACCAAAACA GCCATCAATG 
GACGATTCAG CGGCTCTGGA CATGTTGTCT AGTGATTTTT CAGATGTACC GACTGTAAAG 
CCCACTGTAT CTGATGGCAA AAGCTTCACC CCTGAACCAA CACCACCAAC CTTTAAGGCA 
ACCGGTCCGG TTTTAGACAA ACTGGCAGAC AACGTGCTTT CCAACCTGAC TGACCCCAAA 
GCCAAAGACA GCAAAGCAAA GGGAAAGGGG GCGAAACCAA AGTCTAAACC AAAGAAACAG 
GCAGTGGAAG ACTCTTCAGC CACAGATCTG CTGTCCAGTA AACCGAGCTC AGATGTGGTG 
CCTTCAGCTT CCACAAAGGC TGGAAAAAGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208295.1
CDS	38..2230
Translation	MPHKKRCHGRKKNKKENGDTSFQKQEYAPQTSRFMSQQVTPTTTSQVSTVKPAQYEVQVEVGPSAAKADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_017352806.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352806.1

ORF Insert Sequence:

ATGCCGCACA AAAAGAGATG TCATGGTCGT AAGAAAAACA AAAAGGAGAA TGGAGACACT 
TCATTTCAAA AACAAGAATA CGCTCCACAG ACATCAAGGT TCATGTCTCA GCAGGTCACA 
CCCACCACAA CATCTCAGGT CTCCACAGTG AAACCTGCAC AATATGAGGT GCAGGTTGAA 
GTGGGTCCTT CAGCAGCAAA AGCTGACGAT CCGTTTGATG CCCTGTCTGG CACATTACCA 
TCATCACAAC CTTTGGCTCC TAAAGTCCAC TACACTGGAC CTGAGATCAA AGAGCCCAAA 
CTGAAATCAG AGAAAGGTGT TTTGTGTGGT GAGCGAGATG ACACGCTGCC GCCCGGATAC 
AGAAAGGAAG ACCTGGAAAA GAAGCATGGA GCTGGAGTTC CAGCTGGAGT TCCGGAGAAG 
CCTAAAGAAG TTCCCAAGCC CATAAGCACA GATGATGCAC TGGAATCTCT CTCCTCTACG 
TTTGTGTCTC CAGCTCAAGC TGCTCCAAAG AAGACAGACG TGAAAACTGA AACAATAGGA 
GCAGTGGATG TTAAATCGGC AGGCACCACT AACTTTGCTC CCCCTCCACC ATCTCAGCAG 
AAACAACCGG CGTCGTCTCA AGCTGCTGCT GTAACCAAAT CACCTGCTCC ACCGGCAGAC 
AAGAAAGCTA AAATGGAGAC TCATGCACAA CCAGCCAAAG CAAAGACAGA GTCGGACAGC 
TCCATATCGC TGGACGCTCT CGATGCTTTG AGCGACACAC TGGGGGCTTC TAAACCACCC 
CAAAAATTAC CAGAACTCAA ACCTGGACAG ATAGTTGATG AGAAGAAACA GACCTCAGAG 
AAAGGTGTTC GTGTTGGAGA AAGAGACGAC ACACTTCCAC CACAATACAG ATTCAAAGAG 
GAAGATCTCA AGAAATATCC TCCACCTGCG AAAGAGCCTT CACTGAACAC AGATGATGCG 
ATGGACATAC TTTCAGGAGA TTTCACGACC CCTGCATCGG CACCTGTTGT CAAAGCTCCT 
GTTCCTCCTG CACAGGACAC AAAGAAGCAT GCTGCAGTGC CTGAGAAGAC CAAAGATATT 
CCCAAGACTA AAGCTGATGC TCTGTCAAGT GATTTCGTGG CTCCGGCACA TTCTGCTCCT 
AAGGTTTCTT CAGCTGCTCC TAAAACCATC CCTCCAGGCC CACCACAAAC CCCAGATGAG 
GATCCTTTCA GTGCTTTGGG CGACACTCTT TCTGCCCCAG AACCACCAAA AAAACAACCT 
GACCTGAAAC CTAAGGACAT CGTACATGAG AAGGATGTGA CGTCAAAGAA AGGAGTTCGT 
GTTGGAGAAA GAGACGACAC ACTCCCACCA GAATACAGAT TCAAAGAGGA AGATCTCAAG 
AAATATCCTC CTCCTCAGAA AGATCCTTCC TTAGATGCTA ATGAAGCTCT GGATATTCTG 
TCTGGGGATT TTACAACTCC TTCCGCACCA TCGGCTGTTT CCCCTGCCTC TAAATCCTCT 
GCCAAGTCTT CAGATTCAGC TTTAGATGCT CTTGCTGGTG ATTTCGTCGC TTCTTCTTCT 
GCGTCTTCAG TTCATTCTGC CGTCTCTGGC CCCCCACGCT CCGACAGACT GTTGTCAGAC 
GGAACCTCAT CAGCTCTAGA TGCCCTGTCA GAAACTCTGG GAGACATTAA AGCAGCCCCT 
GAACCCGCCC CTGTCCTACC TAAAACTGTC GTTAAGGAGA AGACGGTCAT AGAAGAGAGA 
GTGAGTAAAC CGGGTGAGAG AGACGACTCC CTTCCACCAG ATCAGCGGTT CACTGCGGAG 
GATCGCAAGG CATTTGAAGA AGCAAAGAAG AAAGACGCCA AACCAAAACA GCCATCAATG 
GACGATTCAG CGGCTCTGGA CATGTTGTCT AGTGATTTTT CAGATGTACC GACTGTAAAG 
CCCACTGTAT CTGATGGCAA AAGCTTCACC CCTGAACCAA CACCACCAAC CTTTAAGGCA 
ACCGGTCCGG TTTTAGACAA ACTGGCAGAC AACGTGCTTT CCAACCTGAC TGACCCCAAA 
GCCAAAGACA GCAAAGCAAA GGGAAAGGGG GCGAAACCAA AGTCTAAACC AAAGAAACAG 
GCAGTGGAAG ACTCTTCAGC CACAGATCTG CTGTCCAGTA AACCGAGCTC AGATGTGGTG 
CCTTCAGCTT CCACAAAGGC TGGAAAAAGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_017352808.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208297.1 Danio rerio calpastatin (cast), transcript variant X26, mRNA.

Price & Availability↑

CloneID	ODa56803
Clone ID Related Accession (Same CDS sequence)	XM_017352808.1 , XM_017352808.2 , XM_021468883.1
Accession Version	XM_017352808.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	calpastatin isoform X26
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC AGGTCACACC CACCACAACA TCTCAGGTCT CCACAGTGAA ACCTGCACAA 
TATGAGGTGC AGGTTGAAGT GGGTCCTTCA GCAGCAAAAG CTGACGATCC GTTTGATGCC 
CTGTCTGGCA CATTACCATC ATCACAACCT TTGGCTCCTA AAGTCCACTA CACTGGACCT 
GAGATCAAAG AGCCCAAACT GAAATCAGAG AAAGGTGTTT TGTGTGGTGA GCGAGATGAC 
ACGCTGCCGC CCGGATACAG AAAGGAAGAC CTGGAAAAGA AGCATGGAGC TGGAGTTCCA 
GCTGGAGTTC CGGAGAAGCC TAAAGAAGTT CCCAAGCCCA TAAGCACAGA TGATGCACTG 
GAATCTCTCT CCTCTACGTT TGTGTCTCCA GCTCAAGCTG CTCCAAAGAA GACAGACGTG 
AAAACTGAAA CAATAGGAGC AGTGGATGTT AAATCGGCAG GCACCACTAA CTTTGCTCCC 
CCTCCACCAT CTCAGCAGAA ACAACCGGCG TCGTCTCAAG CTGCTGCTGT AACCAAATCA 
CCTGCTCCAC CGGCAGACAA GAAAGCTAAA ATGGAGACTC ATGCACAACC AGCCAAAGCA 
AAGACAGAGT CGGACAGCTC CATATCGCTG GACGCTCTCG ATGCTTTGAG CGACACACTG 
GGGGCTTCTA AACCACCCCA AAAATTACCA GAACTCAAAC CTGGACAGAT AGTTGATGAG 
AAGAAACAGA CCTCAGAGAA AGGTGTTCGT GTTGGAGAAA GAGACGACAC ACTTCCACCA 
CAATACAGAT TCAAAGAGGA AGATCTCAAG AAATATCCTC CACCTGCGAA AGAGCCTTCA 
CTGAACACAG ATGATGCGAT GGACATACTT TCAGGAGATT TCACGACCCC TGCATCGGCA 
CCTGTTGTCA AAGCTCCTGT TCCTCCTGCA CAGGACACAA AGAAGCATGC TGCAGTGCCT 
GAGAAGACCA AAGATATTCC CAAGACTAAA GCTGATGCTC TGTCAAGTGA TTTCGTGGCT 
CCGGCACATT CTGCTCCTAA GGTTTCTTCA GCTGCTCCTA AAACCATCCC TCCAGGCCCA 
CCACAAACCC CAGATGAGGA TCCTTTCAGT GCTTTGGGCG ACACTCTTTC TGCCCCAGAA 
CCACCAAAAA AACAACCTGA CCTGAAACCT AAGGACATCG TACATGAGAA GGATGTGACG 
TCAAAGAAAG GAGTTCGTGT TGGAGAAAGA GACGACACAC TCCCACCAGA ATACAGATTC 
AAAGAGGAAG ATCTCAAGAA ATATCCTCCT CCTCAGAAAG ATCCTTCCTT AGATGCTAAT 
GAAGCTCTGG ATATTCTGTC TGGGGATTTT ACAACTCCTT CCGCACCATC GGCTGTTTCC 
CCTGCCTCTA AATCCTCTGC CAAGTCTTCA GATTCAGCTT TAGATGCTCT TGCTGGTGAT 
TTCGTCGCTT CTTCTTCTGC GTCTTCAGTT CATTCTGCCG TCTCTGGCCC CCCACGCTCC 
GACAGACTGT TGTCAGACGG AACCTCATCA GCTCTAGATG CCCTGTCAGA AACTCTGGGA 
GACATTAAAG CAGCCCCTGA ACCCGCCCCT GTCCTACCTA AAACTGTCGT TAAGGAGAAG 
ACGGTCATAG AAGAGAGAGT GAGTAAACCG GGTGAGAGAG ACGACTCCCT TCCACCAGAT 
CAGCGGTTCA CTGCGGAGGA TCGCAAGGCA TTTGAAGAAG CAAAGAAGAA AGACGCCAAA 
CCAAAACAGC CATCAATGGA CGATTCAGCG GCTCTGGACA TGTTGTCTAG TGATTTTTCA 
GATGTACCGA CTGTAAAGCC CACTGTATCT GATGGCAAAA GCTTCACCCC TGAACCAACA 
CCACCAACCT TTAAGGCAAC CGGTCCGGTT TTAGACAAAC TGGCAGACAA CGTGCTTTCC 
AACCTGACTG ACCCCAAAGC CAAAGACAGC AAAGCAAAGG GAAAGGGGGC GAAACCAAAG 
TCTAAACCAA AGAAACAGGC AGTGGAAGAC TCTTCAGCCA CAGATCTGCT GTCCAGTAAA 
CCGAGCTCAG ATGTGGTGCC TTCAGCTTCC ACAAAGGCTG GAAAAAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208297.1
CDS	229..2319
Translation	MSQQVTPTTTSQVSTVKPAQYEVQVEVGPSAAKADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_017352808.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352808.1

ORF Insert Sequence:

ATGTCTCAGC AGGTCACACC CACCACAACA TCTCAGGTCT CCACAGTGAA ACCTGCACAA 
TATGAGGTGC AGGTTGAAGT GGGTCCTTCA GCAGCAAAAG CTGACGATCC GTTTGATGCC 
CTGTCTGGCA CATTACCATC ATCACAACCT TTGGCTCCTA AAGTCCACTA CACTGGACCT 
GAGATCAAAG AGCCCAAACT GAAATCAGAG AAAGGTGTTT TGTGTGGTGA GCGAGATGAC 
ACGCTGCCGC CCGGATACAG AAAGGAAGAC CTGGAAAAGA AGCATGGAGC TGGAGTTCCA 
GCTGGAGTTC CGGAGAAGCC TAAAGAAGTT CCCAAGCCCA TAAGCACAGA TGATGCACTG 
GAATCTCTCT CCTCTACGTT TGTGTCTCCA GCTCAAGCTG CTCCAAAGAA GACAGACGTG 
AAAACTGAAA CAATAGGAGC AGTGGATGTT AAATCGGCAG GCACCACTAA CTTTGCTCCC 
CCTCCACCAT CTCAGCAGAA ACAACCGGCG TCGTCTCAAG CTGCTGCTGT AACCAAATCA 
CCTGCTCCAC CGGCAGACAA GAAAGCTAAA ATGGAGACTC ATGCACAACC AGCCAAAGCA 
AAGACAGAGT CGGACAGCTC CATATCGCTG GACGCTCTCG ATGCTTTGAG CGACACACTG 
GGGGCTTCTA AACCACCCCA AAAATTACCA GAACTCAAAC CTGGACAGAT AGTTGATGAG 
AAGAAACAGA CCTCAGAGAA AGGTGTTCGT GTTGGAGAAA GAGACGACAC ACTTCCACCA 
CAATACAGAT TCAAAGAGGA AGATCTCAAG AAATATCCTC CACCTGCGAA AGAGCCTTCA 
CTGAACACAG ATGATGCGAT GGACATACTT TCAGGAGATT TCACGACCCC TGCATCGGCA 
CCTGTTGTCA AAGCTCCTGT TCCTCCTGCA CAGGACACAA AGAAGCATGC TGCAGTGCCT 
GAGAAGACCA AAGATATTCC CAAGACTAAA GCTGATGCTC TGTCAAGTGA TTTCGTGGCT 
CCGGCACATT CTGCTCCTAA GGTTTCTTCA GCTGCTCCTA AAACCATCCC TCCAGGCCCA 
CCACAAACCC CAGATGAGGA TCCTTTCAGT GCTTTGGGCG ACACTCTTTC TGCCCCAGAA 
CCACCAAAAA AACAACCTGA CCTGAAACCT AAGGACATCG TACATGAGAA GGATGTGACG 
TCAAAGAAAG GAGTTCGTGT TGGAGAAAGA GACGACACAC TCCCACCAGA ATACAGATTC 
AAAGAGGAAG ATCTCAAGAA ATATCCTCCT CCTCAGAAAG ATCCTTCCTT AGATGCTAAT 
GAAGCTCTGG ATATTCTGTC TGGGGATTTT ACAACTCCTT CCGCACCATC GGCTGTTTCC 
CCTGCCTCTA AATCCTCTGC CAAGTCTTCA GATTCAGCTT TAGATGCTCT TGCTGGTGAT 
TTCGTCGCTT CTTCTTCTGC GTCTTCAGTT CATTCTGCCG TCTCTGGCCC CCCACGCTCC 
GACAGACTGT TGTCAGACGG AACCTCATCA GCTCTAGATG CCCTGTCAGA AACTCTGGGA 
GACATTAAAG CAGCCCCTGA ACCCGCCCCT GTCCTACCTA AAACTGTCGT TAAGGAGAAG 
ACGGTCATAG AAGAGAGAGT GAGTAAACCG GGTGAGAGAG ACGACTCCCT TCCACCAGAT 
CAGCGGTTCA CTGCGGAGGA TCGCAAGGCA TTTGAAGAAG CAAAGAAGAA AGACGCCAAA 
CCAAAACAGC CATCAATGGA CGATTCAGCG GCTCTGGACA TGTTGTCTAG TGATTTTTCA 
GATGTACCGA CTGTAAAGCC CACTGTATCT GATGGCAAAA GCTTCACCCC TGAACCAACA 
CCACCAACCT TTAAGGCAAC CGGTCCGGTT TTAGACAAAC TGGCAGACAA CGTGCTTTCC 
AACCTGACTG ACCCCAAAGC CAAAGACAGC AAAGCAAAGG GAAAGGGGGC GAAACCAAAG 
TCTAAACCAA AGAAACAGGC AGTGGAAGAC TCTTCAGCCA CAGATCTGCT GTCCAGTAAA 
CCGAGCTCAG ATGTGGTGCC TTCAGCTTCC ACAAAGGCTG GAAAAAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_017352808.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208297.1 Danio rerio calpastatin (cast), transcript variant X34, mRNA.

Price & Availability↑

CloneID	ODa56803
Clone ID Related Accession (Same CDS sequence)	XM_017352808.1 , XM_017352808.2 , XM_021468883.1
Accession Version	XM_017352808.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calpastatin isoform X34
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352808.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC AGGTCACACC CACCACAACA TCTCAGGTCT CCACAGTGAA ACCTGCACAA 
TATGAGGTGC AGGTTGAAGT GGGTCCTTCA GCAGCAAAAG CTGACGATCC GTTTGATGCC 
CTGTCTGGCA CATTACCATC ATCACAACCT TTGGCTCCTA AAGTCCACTA CACTGGACCT 
GAGATCAAAG AGCCCAAACT GAAATCAGAG AAAGGTGTTT TGTGTGGTGA GCGAGATGAC 
ACGCTGCCGC CCGGATACAG AAAGGAAGAC CTGGAAAAGA AGCATGGAGC TGGAGTTCCA 
GCTGGAGTTC CGGAGAAGCC TAAAGAAGTT CCCAAGCCCA TAAGCACAGA TGATGCACTG 
GAATCTCTCT CCTCTACGTT TGTGTCTCCA GCTCAAGCTG CTCCAAAGAA GACAGACGTG 
AAAACTGAAA CAATAGGAGC AGTGGATGTT AAATCGGCAG GCACCACTAA CTTTGCTCCC 
CCTCCACCAT CTCAGCAGAA ACAACCGGCG TCGTCTCAAG CTGCTGCTGT AACCAAATCA 
CCTGCTCCAC CGGCAGACAA GAAAGCTAAA ATGGAGACTC ATGCACAACC AGCCAAAGCA 
AAGACAGAGT CGGACAGCTC CATATCGCTG GACGCTCTCG ATGCTTTGAG CGACACACTG 
GGGGCTTCTA AACCACCCCA AAAATTACCA GAACTCAAAC CTGGACAGAT AGTTGATGAG 
AAGAAACAGA CCTCAGAGAA AGGTGTTCGT GTTGGAGAAA GAGACGACAC ACTTCCACCA 
CAATACAGAT TCAAAGAGGA AGATCTCAAG AAATATCCTC CACCTGCGAA AGAGCCTTCA 
CTGAACACAG ATGATGCGAT GGACATACTT TCAGGAGATT TCACGACCCC TGCATCGGCA 
CCTGTTGTCA AAGCTCCTGT TCCTCCTGCA CAGGACACAA AGAAGCATGC TGCAGTGCCT 
GAGAAGACCA AAGATATTCC CAAGACTAAA GCTGATGCTC TGTCAAGTGA TTTCGTGGCT 
CCGGCACATT CTGCTCCTAA GGTTTCTTCA GCTGCTCCTA AAACCATCCC TCCAGGCCCA 
CCACAAACCC CAGATGAGGA TCCTTTCAGT GCTTTGGGCG ACACTCTTTC TGCCCCAGAA 
CCACCAAAAA AACAACCTGA CCTGAAACCT AAGGACATCG TACATGAGAA GGATGTGACG 
TCAAAGAAAG GAGTTCGTGT TGGAGAAAGA GACGACACAC TCCCACCAGA ATACAGATTC 
AAAGAGGAAG ATCTCAAGAA ATATCCTCCT CCTCAGAAAG ATCCTTCCTT AGATGCTAAT 
GAAGCTCTGG ATATTCTGTC TGGGGATTTT ACAACTCCTT CCGCACCATC GGCTGTTTCC 
CCTGCCTCTA AATCCTCTGC CAAGTCTTCA GATTCAGCTT TAGATGCTCT TGCTGGTGAT 
TTCGTCGCTT CTTCTTCTGC GTCTTCAGTT CATTCTGCCG TCTCTGGCCC CCCACGCTCC 
GACAGACTGT TGTCAGACGG AACCTCATCA GCTCTAGATG CCCTGTCAGA AACTCTGGGA 
GACATTAAAG CAGCCCCTGA ACCCGCCCCT GTCCTACCTA AAACTGTCGT TAAGGAGAAG 
ACGGTCATAG AAGAGAGAGT GAGTAAACCG GGTGAGAGAG ACGACTCCCT TCCACCAGAT 
CAGCGGTTCA CTGCGGAGGA TCGCAAGGCA TTTGAAGAAG CAAAGAAGAA AGACGCCAAA 
CCAAAACAGC CATCAATGGA CGATTCAGCG GCTCTGGACA TGTTGTCTAG TGATTTTTCA 
GATGTACCGA CTGTAAAGCC CACTGTATCT GATGGCAAAA GCTTCACCCC TGAACCAACA 
CCACCAACCT TTAAGGCAAC CGGTCCGGTT TTAGACAAAC TGGCAGACAA CGTGCTTTCC 
AACCTGACTG ACCCCAAAGC CAAAGACAGC AAAGCAAAGG GAAAGGGGGC GAAACCAAAG 
TCTAAACCAA AGAAACAGGC AGTGGAAGAC TCTTCAGCCA CAGATCTGCT GTCCAGTAAA 
CCGAGCTCAG ATGTGGTGCC TTCAGCTTCC ACAAAGGCTG GAAAAAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208297.1
CDS	79..2169
Translation	MSQQVTPTTTSQVSTVKPAQYEVQVEVGPSAAKADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_017352808.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X34, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352808.2

ORF Insert Sequence:

ATGTCTCAGC AGGTCACACC CACCACAACA TCTCAGGTCT CCACAGTGAA ACCTGCACAA 
TATGAGGTGC AGGTTGAAGT GGGTCCTTCA GCAGCAAAAG CTGACGATCC GTTTGATGCC 
CTGTCTGGCA CATTACCATC ATCACAACCT TTGGCTCCTA AAGTCCACTA CACTGGACCT 
GAGATCAAAG AGCCCAAACT GAAATCAGAG AAAGGTGTTT TGTGTGGTGA GCGAGATGAC 
ACGCTGCCGC CCGGATACAG AAAGGAAGAC CTGGAAAAGA AGCATGGAGC TGGAGTTCCA 
GCTGGAGTTC CGGAGAAGCC TAAAGAAGTT CCCAAGCCCA TAAGCACAGA TGATGCACTG 
GAATCTCTCT CCTCTACGTT TGTGTCTCCA GCTCAAGCTG CTCCAAAGAA GACAGACGTG 
AAAACTGAAA CAATAGGAGC AGTGGATGTT AAATCGGCAG GCACCACTAA CTTTGCTCCC 
CCTCCACCAT CTCAGCAGAA ACAACCGGCG TCGTCTCAAG CTGCTGCTGT AACCAAATCA 
CCTGCTCCAC CGGCAGACAA GAAAGCTAAA ATGGAGACTC ATGCACAACC AGCCAAAGCA 
AAGACAGAGT CGGACAGCTC CATATCGCTG GACGCTCTCG ATGCTTTGAG CGACACACTG 
GGGGCTTCTA AACCACCCCA AAAATTACCA GAACTCAAAC CTGGACAGAT AGTTGATGAG 
AAGAAACAGA CCTCAGAGAA AGGTGTTCGT GTTGGAGAAA GAGACGACAC ACTTCCACCA 
CAATACAGAT TCAAAGAGGA AGATCTCAAG AAATATCCTC CACCTGCGAA AGAGCCTTCA 
CTGAACACAG ATGATGCGAT GGACATACTT TCAGGAGATT TCACGACCCC TGCATCGGCA 
CCTGTTGTCA AAGCTCCTGT TCCTCCTGCA CAGGACACAA AGAAGCATGC TGCAGTGCCT 
GAGAAGACCA AAGATATTCC CAAGACTAAA GCTGATGCTC TGTCAAGTGA TTTCGTGGCT 
CCGGCACATT CTGCTCCTAA GGTTTCTTCA GCTGCTCCTA AAACCATCCC TCCAGGCCCA 
CCACAAACCC CAGATGAGGA TCCTTTCAGT GCTTTGGGCG ACACTCTTTC TGCCCCAGAA 
CCACCAAAAA AACAACCTGA CCTGAAACCT AAGGACATCG TACATGAGAA GGATGTGACG 
TCAAAGAAAG GAGTTCGTGT TGGAGAAAGA GACGACACAC TCCCACCAGA ATACAGATTC 
AAAGAGGAAG ATCTCAAGAA ATATCCTCCT CCTCAGAAAG ATCCTTCCTT AGATGCTAAT 
GAAGCTCTGG ATATTCTGTC TGGGGATTTT ACAACTCCTT CCGCACCATC GGCTGTTTCC 
CCTGCCTCTA AATCCTCTGC CAAGTCTTCA GATTCAGCTT TAGATGCTCT TGCTGGTGAT 
TTCGTCGCTT CTTCTTCTGC GTCTTCAGTT CATTCTGCCG TCTCTGGCCC CCCACGCTCC 
GACAGACTGT TGTCAGACGG AACCTCATCA GCTCTAGATG CCCTGTCAGA AACTCTGGGA 
GACATTAAAG CAGCCCCTGA ACCCGCCCCT GTCCTACCTA AAACTGTCGT TAAGGAGAAG 
ACGGTCATAG AAGAGAGAGT GAGTAAACCG GGTGAGAGAG ACGACTCCCT TCCACCAGAT 
CAGCGGTTCA CTGCGGAGGA TCGCAAGGCA TTTGAAGAAG CAAAGAAGAA AGACGCCAAA 
CCAAAACAGC CATCAATGGA CGATTCAGCG GCTCTGGACA TGTTGTCTAG TGATTTTTCA 
GATGTACCGA CTGTAAAGCC CACTGTATCT GATGGCAAAA GCTTCACCCC TGAACCAACA 
CCACCAACCT TTAAGGCAAC CGGTCCGGTT TTAGACAAAC TGGCAGACAA CGTGCTTTCC 
AACCTGACTG ACCCCAAAGC CAAAGACAGC AAAGCAAAGG GAAAGGGGGC GAAACCAAAG 
TCTAAACCAA AGAAACAGGC AGTGGAAGAC TCTTCAGCCA CAGATCTGCT GTCCAGTAAA 
CCGAGCTCAG ATGTGGTGCC TTCAGCTTCC ACAAAGGCTG GAAAAAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_021468883.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324558.1 Danio rerio calpastatin (cast), transcript variant X33, mRNA.

Price & Availability↑

CloneID	ODa56803
Clone ID Related Accession (Same CDS sequence)	XM_017352808.1 , XM_017352808.2 , XM_021468883.1
Accession Version	XM_021468883.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calpastatin isoform X33
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC AGGTCACACC CACCACAACA TCTCAGGTCT CCACAGTGAA ACCTGCACAA 
TATGAGGTGC AGGTTGAAGT GGGTCCTTCA GCAGCAAAAG CTGACGATCC GTTTGATGCC 
CTGTCTGGCA CATTACCATC ATCACAACCT TTGGCTCCTA AAGTCCACTA CACTGGACCT 
GAGATCAAAG AGCCCAAACT GAAATCAGAG AAAGGTGTTT TGTGTGGTGA GCGAGATGAC 
ACGCTGCCGC CCGGATACAG AAAGGAAGAC CTGGAAAAGA AGCATGGAGC TGGAGTTCCA 
GCTGGAGTTC CGGAGAAGCC TAAAGAAGTT CCCAAGCCCA TAAGCACAGA TGATGCACTG 
GAATCTCTCT CCTCTACGTT TGTGTCTCCA GCTCAAGCTG CTCCAAAGAA GACAGACGTG 
AAAACTGAAA CAATAGGAGC AGTGGATGTT AAATCGGCAG GCACCACTAA CTTTGCTCCC 
CCTCCACCAT CTCAGCAGAA ACAACCGGCG TCGTCTCAAG CTGCTGCTGT AACCAAATCA 
CCTGCTCCAC CGGCAGACAA GAAAGCTAAA ATGGAGACTC ATGCACAACC AGCCAAAGCA 
AAGACAGAGT CGGACAGCTC CATATCGCTG GACGCTCTCG ATGCTTTGAG CGACACACTG 
GGGGCTTCTA AACCACCCCA AAAATTACCA GAACTCAAAC CTGGACAGAT AGTTGATGAG 
AAGAAACAGA CCTCAGAGAA AGGTGTTCGT GTTGGAGAAA GAGACGACAC ACTTCCACCA 
CAATACAGAT TCAAAGAGGA AGATCTCAAG AAATATCCTC CACCTGCGAA AGAGCCTTCA 
CTGAACACAG ATGATGCGAT GGACATACTT TCAGGAGATT TCACGACCCC TGCATCGGCA 
CCTGTTGTCA AAGCTCCTGT TCCTCCTGCA CAGGACACAA AGAAGCATGC TGCAGTGCCT 
GAGAAGACCA AAGATATTCC CAAGACTAAA GCTGATGCTC TGTCAAGTGA TTTCGTGGCT 
CCGGCACATT CTGCTCCTAA GGTTTCTTCA GCTGCTCCTA AAACCATCCC TCCAGGCCCA 
CCACAAACCC CAGATGAGGA TCCTTTCAGT GCTTTGGGCG ACACTCTTTC TGCCCCAGAA 
CCACCAAAAA AACAACCTGA CCTGAAACCT AAGGACATCG TACATGAGAA GGATGTGACG 
TCAAAGAAAG GAGTTCGTGT TGGAGAAAGA GACGACACAC TCCCACCAGA ATACAGATTC 
AAAGAGGAAG ATCTCAAGAA ATATCCTCCT CCTCAGAAAG ATCCTTCCTT AGATGCTAAT 
GAAGCTCTGG ATATTCTGTC TGGGGATTTT ACAACTCCTT CCGCACCATC GGCTGTTTCC 
CCTGCCTCTA AATCCTCTGC CAAGTCTTCA GATTCAGCTT TAGATGCTCT TGCTGGTGAT 
TTCGTCGCTT CTTCTTCTGC GTCTTCAGTT CATTCTGCCG TCTCTGGCCC CCCACGCTCC 
GACAGACTGT TGTCAGACGG AACCTCATCA GCTCTAGATG CCCTGTCAGA AACTCTGGGA 
GACATTAAAG CAGCCCCTGA ACCCGCCCCT GTCCTACCTA AAACTGTCGT TAAGGAGAAG 
ACGGTCATAG AAGAGAGAGT GAGTAAACCG GGTGAGAGAG ACGACTCCCT TCCACCAGAT 
CAGCGGTTCA CTGCGGAGGA TCGCAAGGCA TTTGAAGAAG CAAAGAAGAA AGACGCCAAA 
CCAAAACAGC CATCAATGGA CGATTCAGCG GCTCTGGACA TGTTGTCTAG TGATTTTTCA 
GATGTACCGA CTGTAAAGCC CACTGTATCT GATGGCAAAA GCTTCACCCC TGAACCAACA 
CCACCAACCT TTAAGGCAAC CGGTCCGGTT TTAGACAAAC TGGCAGACAA CGTGCTTTCC 
AACCTGACTG ACCCCAAAGC CAAAGACAGC AAAGCAAAGG GAAAGGGGGC GAAACCAAAG 
TCTAAACCAA AGAAACAGGC AGTGGAAGAC TCTTCAGCCA CAGATCTGCT GTCCAGTAAA 
CCGAGCTCAG ATGTGGTGCC TTCAGCTTCC ACAAAGGCTG GAAAAAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324558.1
CDS	149..2239
Translation	MSQQVTPTTTSQVSTVKPAQYEVQVEVGPSAAKADDPFDALSGTLPSSQPLAPKVHYTGPEIKEPKLKSEKGVLCGERDDTLPPGYRKEDLEKKHGAGVPAGVPEKPKEVPKPISTDDALESLSSTFVSPAQAAPKKTDVKTETIGAVDVKSAGTTNFAPPPPSQQKQPASSQAAAVTKSPAPPADKKAKMETHAQPAKAKTESDSSISLDALDALSDTLGASKPPQKLPELKPGQIVDEKKQTSEKGVRVGERDDTLPPQYRFKEEDLKKYPPPAKEPSLNTDDAMDILSGDFTTPASAPVVKAPVPPAQDTKKHAAVPEKTKDIPKTKADALSSDFVAPAHSAPKVSSAAPKTIPPGPPQTPDEDPFSALGDTLSAPEPPKKQPDLKPKDIVHEKDVTSKKGVRVGERDDTLPPEYRFKEEDLKKYPPPQKDPSLDANEALDILSGDFTTPSAPSAVSPASKSSAKSSDSALDALAGDFVASSSASSVHSAVSGPPRSDRLLSDGTSSALDALSETLGDIKAAPEPAPVLPKTVVKEKTVIEERVSKPGERDDSLPPDQRFTAEDRKAFEEAKKKDAKPKQPSMDDSAALDMLSSDFSDVPTVKPTVSDGKSFTPEPTPPTFKATGPVLDKLADNVLSNLTDPKAKDSKAKGKGAKPKSKPKKQAVEDSSATDLLSSKPSSDVVPSASTKAGKR

Target ORF information:

RefSeq Version	XM_021468883.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpastatin (cast), transcript variant X33, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468883.1

ORF Insert Sequence:

ATGTCTCAGC AGGTCACACC CACCACAACA TCTCAGGTCT CCACAGTGAA ACCTGCACAA 
TATGAGGTGC AGGTTGAAGT GGGTCCTTCA GCAGCAAAAG CTGACGATCC GTTTGATGCC 
CTGTCTGGCA CATTACCATC ATCACAACCT TTGGCTCCTA AAGTCCACTA CACTGGACCT 
GAGATCAAAG AGCCCAAACT GAAATCAGAG AAAGGTGTTT TGTGTGGTGA GCGAGATGAC 
ACGCTGCCGC CCGGATACAG AAAGGAAGAC CTGGAAAAGA AGCATGGAGC TGGAGTTCCA 
GCTGGAGTTC CGGAGAAGCC TAAAGAAGTT CCCAAGCCCA TAAGCACAGA TGATGCACTG 
GAATCTCTCT CCTCTACGTT TGTGTCTCCA GCTCAAGCTG CTCCAAAGAA GACAGACGTG 
AAAACTGAAA CAATAGGAGC AGTGGATGTT AAATCGGCAG GCACCACTAA CTTTGCTCCC 
CCTCCACCAT CTCAGCAGAA ACAACCGGCG TCGTCTCAAG CTGCTGCTGT AACCAAATCA 
CCTGCTCCAC CGGCAGACAA GAAAGCTAAA ATGGAGACTC ATGCACAACC AGCCAAAGCA 
AAGACAGAGT CGGACAGCTC CATATCGCTG GACGCTCTCG ATGCTTTGAG CGACACACTG 
GGGGCTTCTA AACCACCCCA AAAATTACCA GAACTCAAAC CTGGACAGAT AGTTGATGAG 
AAGAAACAGA CCTCAGAGAA AGGTGTTCGT GTTGGAGAAA GAGACGACAC ACTTCCACCA 
CAATACAGAT TCAAAGAGGA AGATCTCAAG AAATATCCTC CACCTGCGAA AGAGCCTTCA 
CTGAACACAG ATGATGCGAT GGACATACTT TCAGGAGATT TCACGACCCC TGCATCGGCA 
CCTGTTGTCA AAGCTCCTGT TCCTCCTGCA CAGGACACAA AGAAGCATGC TGCAGTGCCT 
GAGAAGACCA AAGATATTCC CAAGACTAAA GCTGATGCTC TGTCAAGTGA TTTCGTGGCT 
CCGGCACATT CTGCTCCTAA GGTTTCTTCA GCTGCTCCTA AAACCATCCC TCCAGGCCCA 
CCACAAACCC CAGATGAGGA TCCTTTCAGT GCTTTGGGCG ACACTCTTTC TGCCCCAGAA 
CCACCAAAAA AACAACCTGA CCTGAAACCT AAGGACATCG TACATGAGAA GGATGTGACG 
TCAAAGAAAG GAGTTCGTGT TGGAGAAAGA GACGACACAC TCCCACCAGA ATACAGATTC 
AAAGAGGAAG ATCTCAAGAA ATATCCTCCT CCTCAGAAAG ATCCTTCCTT AGATGCTAAT 
GAAGCTCTGG ATATTCTGTC TGGGGATTTT ACAACTCCTT CCGCACCATC GGCTGTTTCC 
CCTGCCTCTA AATCCTCTGC CAAGTCTTCA GATTCAGCTT TAGATGCTCT TGCTGGTGAT 
TTCGTCGCTT CTTCTTCTGC GTCTTCAGTT CATTCTGCCG TCTCTGGCCC CCCACGCTCC 
GACAGACTGT TGTCAGACGG AACCTCATCA GCTCTAGATG CCCTGTCAGA AACTCTGGGA 
GACATTAAAG CAGCCCCTGA ACCCGCCCCT GTCCTACCTA AAACTGTCGT TAAGGAGAAG 
ACGGTCATAG AAGAGAGAGT GAGTAAACCG GGTGAGAGAG ACGACTCCCT TCCACCAGAT 
CAGCGGTTCA CTGCGGAGGA TCGCAAGGCA TTTGAAGAAG CAAAGAAGAA AGACGCCAAA 
CCAAAACAGC CATCAATGGA CGATTCAGCG GCTCTGGACA TGTTGTCTAG TGATTTTTCA 
GATGTACCGA CTGTAAAGCC CACTGTATCT GATGGCAAAA GCTTCACCCC TGAACCAACA 
CCACCAACCT TTAAGGCAAC CGGTCCGGTT TTAGACAAAC TGGCAGACAA CGTGCTTTCC 
AACCTGACTG ACCCCAAAGC CAAAGACAGC AAAGCAAAGG GAAAGGGGGC GAAACCAAAG 
TCTAAACCAA AGAAACAGGC AGTGGAAGAC TCTTCAGCCA CAGATCTGCT GTCCAGTAAA 
CCGAGCTCAG ATGTGGTGCC TTCAGCTTCC ACAAAGGCTG GAAAAAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Functional characterization of zebrafish orthologs of the human Beta 3-Glucosyltransferase B3GLCT gene mutated in Peters Plus Syndrome.
PloS one12(9)e0184903(2017)
Weh E,Takeuchi H,Muheisen S,Haltiwanger RS,Semina EV

Ribosomal Proteins Rpl22 and Rpl22l1 Control Morphogenesis by Regulating Pre-mRNA Splicing.
Cell reports18(2)545-556(2017 01)
Zhang Y,O'Leary MN,Peri S,Wang M,Zha J,Melov S,Kappes DJ,Feng Q,Rhodes J,Amieux PS,Morris DR,Kennedy BK,Wiest DL

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Phenotype anchoring in zebrafish reveals a potential role for matrix metalloproteinases (MMPs) in tamoxifen's effects on skin epithelium.
Toxicology and applied pharmacology29631-41(2016 Apr)
Bugel SM,Wehmas LC,La Du JK,Tanguay RL

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cast cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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