shroom4 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	shroom4
Entrez Gene ID	559035
Full Name	shroom family member 4
Synonyms	im:7145757,wu:fe05h01,zgc:194172,zgc:194173
General protein information	Preferred Names shroom family member 4 Names protein Shroom4 shroom family member 4
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009303139.2	XP_009301414.1	protein Shroom4 isoform X2
XM_682334.8	XP_687426.5	protein Shroom4 isoform X3
XM_005172333.4	XP_005172390.1	protein Shroom4 isoform X1
XM_005172333.3	XP_005172390.1	protein Shroom4 isoform X1
XM_682334.9	XP_687426.5	protein Shroom4 isoform X2

Pathways from BioSystems
Gene Ontology

The following shroom4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the shroom4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa12970	XM_005172333.4 Latest version!	Danio rerio shroom family member 4 (shroom4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa12972	XM_682334.9 Latest version!	Danio rerio shroom family member 4 (shroom4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa42960	XM_009303139.2 Latest version!	Danio rerio shroom family member 4 (shroom4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
ODa12970	XM_005172333.3	Danio rerio shroom family member 4 (shroom4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa12972	XM_682334.8	Danio rerio shroom family member 4 (shroom4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

shroom4 ( XM_005172333.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172390.1 Danio rerio shroom family member 4 (shroom4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa12970
Clone ID Related Accession (Same CDS sequence)	XM_005172333.4 , XM_005172333.3
Accession Version	XM_005172333.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein Shroom4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005172333.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACCG TAGAGCAGCT GGTGTCTTTC CAACACATCC ATGTGCAGCT GAACGGAGGC 
GCTCCATGGG GATTCACTCT AAAAGGAGGA CTGGAGCACG GCGAACCACT CATCATCACT 
AAAATAGAAG AGGGAGGAAA AGCAGCTCAG TGTAAGAAGT TGAGAGTGGG GGATGAACTG 
GTGAACATCA ACGGCTCTGC TCTCTACGGC TCACGGCAAG AAGCTCTCAT TCTCATCAAA 
GGTTCCTACA GGGTTCTGAA GATCATCGTC CGAAGGCGCA GTGTGCCAGT GATCCGCCCT 
CACTCTTGGC ACCTGGCAAA GCTGACAGAG GCACCTTGCA CTACGGGCAC TGGTGATCCA 
TCCGATGGCC CTCCGGCTAT GCAGCTTCAC TTTACACCTC TCAATGTACC TTGGCACTCC 
GGAGGTGACA ACAGTGAGCT GTCCATGCAG TGGGGCCATA TCTCCAGGCA CTACAGCACT 
GACCGCAGCA GCTCAATAGG GAGTATGGAG AGTCTGGAGA ATCCTCCTAA CCAGGGATAC 
TATGACAGTC AGCTCTCTCC CATTGATCCT GTCATCTTTA ACAACAAACG TGACTCTGCT 
TACAGCTCTT TCTCAGCCAG CTCGAACACA TCTGATTACA CAGTTCCTGT CAAACCTGAG 
GAGACCAATT CCATGGACAG TCTTCTTCAG GGTTTGGGTT CTTCCTGCAG GTATCCAGAC 
AGAGGCCAAC ACTCGGCCCC CACTGGCCAT GGAAACCAGC TGGAAGAACA AGAACACTCC 
AAGGTGCTGT CTGGCAGATC CGAGCCCAAA GTCCGACCAT CGTCCTACAG CTGTGAGGAG 
GAAAACTGTG CACCACCACA GCCACCAATG AGGAAGGAGA GTTTTAGGGC TACTAGAGGC 
CAAGCAACAG ATAAACGCTG TGTGTCTGCT CCTGTAGGCA TCCCAAATGT AAGCAGTTGC 
ACGGATGAAG ATCAATCCCA AATCCAGGAT GTTTTGACTG GCAGAGTTTA TTTGAACGGA 
ATACAAGACA GTGAGCCAGA GCGTAAAGGG TGCAGCATTC AACCTTATTA TACCTTGAAC 
TCAGACTCAG GTGGAGATGG CAAAGCAACT GACCATGAGA AAGAACACTT GGAGGACTAT 
TCAAAATCTG TGGCAGCAGC ATCTTCACCT CCTTCACAAA GGAATAGGGA TGAGAACTTT 
GATACCAAAC TCCGACCTGA ACATGATCTC CAGTCAGCAA TGAACCGACA TAGTGCACCT 
GAGAAACTTC TAGCTGCTCA GTTGTGTATG ATGGATGTTT CTGCTGACAA AAGTGATCAT 
AGTGCATCTC CAACTTGCCA GTGGTCACAG TCCCCTCTTT ATCTGAGTGA TTCCAGTCAA 
AATACTGAGC AGGGCAAGTG GGGAACGAGC AGATGCTCTA CACCTGGTTC GGTCGCAACT 
TCGGAAGTCG AAGATCCCAG ACTAGAGGAG GAATCTATTG ATGGACAGAA TCCATGGGGG 
CATCCTATCA GTTTGTCTGG GAGTCCCATT GGAAATGGAT TCTCTCAGAA CAGTGAGAAC 
AATGGAGGAG AAGTAATGCT GGATGATGGC CTTGGCCCAA AGCAGCCACA GAAACGTCAG 
TTTCGTTGCT CCAAGTCTCG TCGCAGAAGT GAACGTTTTG CCACGAACCT CAGAAATGAG 
ATCCAGAGGA AGAAGGCACA GTTGCAGAAG AGTAGAAACC CTTGCGGGGA AGAGACAGTG 
GAAGAAGAGG TTGGAGATTT CAACACTGAG GTTGTAGCTC CTCCAGAATA CCGTCAACAT 
AAACCCCAAA TCTTACCAAA ACCTGCCACC AATACTGTAC CTATACAAAT ATCTAAACCG 
GCAACTCAAA AAGTTTTTGA TGCCACACAA ACTGAAGAAT ATTCCAGTGC CAGGGTTGTG 
TGTTTCCAGA CATCTCAGAC TCAAACACAA GTCCCTCAAC ATGTCAGACA AGTGTGTGTA 
CATGTGGTAG AGGAGGTAGC ACCTGCAGGC AAACCACGTC GGTGGCGTTG GACGCCAGAA 
CGCAAACTTG AACTCGAAAA TGAATCATCT GAAAGTAAGA GGAATGAGAC TATTGGACAG 
ATGTGGTCTG GAAAAATGGA ATCAACAAGA AGTAGGACGA GTTCATCCAG CGGTCGTTTA 
GGTCGATCGG ATGACTGTGA CATCCTTCCC TTTGCAGATC GCAGGAGGTT TTTTGAGGAG 
ACTAGCAGAA AGTTGAGCCA GTCTGTAACA AATTTATCAA ACCTGACAAG TCGAAGTCAG 
AGACTAGACA AGCCGGGCAG AATGAACCAT CCATCATCAC CTGAACCTCA TGAGATTGCT 
GCTCACCTTG GCCGTAGGAG ATTCTCATAT CAAGATGTGC CCTACATCAA CTCACTGGAC 
AATGGGAGAC AGTTTATAAG TAACCAACAA GACCAGGAAA AAACGAGGCA GAGGCTGATT 
GAGAGAGAGC AGGAGAGGGA AAGAGAGCAG GAGAGACTTA AGGAGCAAGA GAAGCTTAGA 
GAACAAGAAA GAGAAAAGGA GAGGTTAAGG AAAGAGAGGG AAAATGAGCA ACAAAGGCTG 
AAGGACTGGG AGGAACGGCA GAGGCTGCTA CAGAGAGAAC AAGAATGGGA GTCTGTGAGA 
GAATCTGTTC ACTCTGAACA CAATACGAGC AGTGACACAG TTCCTCACTC TTTATCTCAT 
GACGTCTATC GGAAGCAGCC GCCTAGTCCT CAGATTCCTT CCTCTCTGCA AATGTCTGAA 
CCTTTATATA GCAGCACTAC CACCAACGTC CAAAAGCCTT GCTCAGCGTT CCACCCTGTG 
ACCACCCAGC ACAACCGGTA TGATGGCTAC CAAGTAAATC CACCCTACAA TCCCAGGAAC 
TACACCCCAG ATGAGGCCCA GTATGTTCGG GAACAGGAAA AAACTAGAAA GATCAACAGA 
AACTTCAGCT TAACAGAGAG GGACTACCCA AGGTCCAGGA GAGACTTCAA GACAGTAGAT 
GGTGCTAATG TCCCCGGCTT CGTGCGCACA GGAGGCAACA GCATCACAAG TGGACGCAAC 
GAGGAGCATC TTTTTACCCC TCTTAGAAAC CGTGCCATGT CAGAGAATAA CCTGTGTGTG 
GAGACACAAC ATTATCAAAA TCACAGCTCC AGCTCTGGCG TCAGCAGAAT GCGCTCGTCC 
ACTCTTAGTG ACCTGGATGA AAATACAGTA AGTGTCGGGG AGATCAAAAA GAAAAAAGGG 
CCTCCGCCGC CTCGTCCACC ACCACCAAAA TGGGATCAGT TCCACAAGAG ACGGGCGTCC 
CATCACAGCC TGTTCTCCTC TACATCACAC TACTCCTCTC CAACTGCTTC TCCACCTCGG 
CCACAGCCAC AGACGTGCAC ATCACGACCC CCCAGTGTCT CTGAAATGAG CCGTCAGCGC 
TCTTACAGTC TTCCTCCAAG AGACATGTCC GAGAGTCTTC ACTGCTGCCG TCAGGAGTAC 
TCCGTCGCTC CCTCCAGTCC TGCTTTTACA CGTCGAGCTT TTAAGCCTGT AGCCCTGCCG 
CCACGAGAGA TGGACTATAA CACGAGGAGA GAAGTTCACC AGCCTGAACC ATGCACCAGA 
ATCCCCATCC ATCCTGCTGC AGCACCAGAA GAGCCCAGAG CCACACATCT GAAGCCCACA 
TTCCCTGATC ATGGAGTGGA GTGGGATCGA TCCAGCCCTC ACTACCCCAC ACATGGCACA 
AGCAGGACTC CTGAAGTGCC CTCAAACAGT TTCTCAGCTG GGCCTTTATG TCCAGAGTCC 
TACTTCTCCA TGAACAACTT CCAACTGCAG CCCCAGCAAG CAGGCTTCCC CATAACAGCC 
CACAAAACCC AGATCATCCC CTCTCAGGGT CCTGAAGATG GAGACCTGCC TCTGGAGACG 
GACATCGATG AGATCTGTGA GAATGAGCAG GCTGAGAGGA AGGAGACGGT GGACCGGATG 
GAGATGCAGG GCTTTGCTCG ACCTGTGATG GTGTTGGAAA CCGACATTGA CCACACGCCT 
GAGGAAGCGG CTCCATCGGC CATAAGCATT AGAGGGCCGA GGGGCTCCCT GGTGGACTCC 
ATTTTGGAGG ATGAGTACGG TGTGTCTAGG AAGGAGCTGC TTGGAGAGTT GTTTCCACAC 
AGTGTGAATG CTGAGATGAG TGGGGATGGC TGGAGAGGAG GATATCCCAT CAGTGGAGGA 
ACACTGGAGA GACCCTCCAG ACTGAATACC ACAGCGCCAC AAGTATCACG ATCCACCTGT 
TATGACATAT CAGCAGACAA CCCACAGCTT CTCGCCAAGC TTAGAGAGAT CTCAGAGAGG 
AAAGAGGAAG ATGAGGAGCT CAATTATAAG AAGCAGCTGA TGGAGAGTCT GCGTAAAAAG 
CTGACTGTCC TGCGTGAGGC ACAGCGGGGT CTGCAGGAGG ACATTCGGGC CAATGCTCAG 
CTGGGAGATG AGGTGGAGAG TCTGGTGCTG GCCATCTGCA AGCCCAACGA GGTGGACAAG 
TATCGGATGT TCATCGGCGA CCTTGACAAG GTGACCAGTC TGCTGCTGTC GCTTTCCGGA 
AGGCTGATCC GAGTGGAAAG TGCCCTGGAC TGTGTGGACC CAGAAACTGG ACACCAGGAG 
AGGCTCCAAC TCCTGGAGAA GAAGAAGCAG CTGTTGGTTC AGATGGGAGA GGCGCAGGAG 
CTGAAGGAGC ACGTGGACCG GCGCGAGCAG GCCGTCTGCA GAGTGCTGGG ATGCTGCCTG 
ACCCCAGAAC AGATGCGGGA CTACGGTCAC TTTGTGAAGA TGAAGGCGGC GCTACTGGTA 
GAGCAGAGGC AACTGGATGA CAAGATACGG CTCGGCGAGG AGCAGCTCAG GGGACTTCGA 
GAGAGCCTCG GCCTGGGTTT TGGGATGGCG TATGGCCATT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172390.1
CDS	153..5057
Translation	METVEQLVSFQHIHVQLNGGAPWGFTLKGGLEHGEPLIITKIEEGGKAAQCKKLRVGDELVNINGSALYGSRQEALILIKGSYRVLKIIVRRRSVPVIRPHSWHLAKLTEAPCTTGTGDPSDGPPAMQLHFTPLNVPWHSGGDNSELSMQWGHISRHYSTDRSSSIGSMESLENPPNQGYYDSQLSPIDPVIFNNKRDSAYSSFSASSNTSDYTVPVKPEETNSMDSLLQGLGSSCRYPDRGQHSAPTGHGNQLEEQEHSKVLSGRSEPKVRPSSYSCEEENCAPPQPPMRKESFRATRGQATDKRCVSAPVGIPNVSSCTDEDQSQIQDVLTGRVYLNGIQDSEPERKGCSIQPYYTLNSDSGGDGKATDHEKEHLEDYSKSVAAASSPPSQRNRDENFDTKLRPEHDLQSAMNRHSAPEKLLAAQLCMMDVSADKSDHSASPTCQWSQSPLYLSDSSQNTEQGKWGTSRCSTPGSVATSEVEDPRLEEESIDGQNPWGHPISLSGSPIGNGFSQNSENNGGEVMLDDGLGPKQPQKRQFRCSKSRRRSERFATNLRNEIQRKKAQLQKSRNPCGEETVEEEVGDFNTEVVAPPEYRQHKPQILPKPATNTVPIQISKPATQKVFDATQTEEYSSARVVCFQTSQTQTQVPQHVRQVCVHVVEEVAPAGKPRRWRWTPERKLELENESSESKRNETIGQMWSGKMESTRSRTSSSSGRLGRSDDCDILPFADRRRFFEETSRKLSQSVTNLSNLTSRSQRLDKPGRMNHPSSPEPHEIAAHLGRRRFSYQDVPYINSLDNGRQFISNQQDQEKTRQRLIEREQEREREQERLKEQEKLREQEREKERLRKERENEQQRLKDWEERQRLLQREQEWESVRESVHSEHNTSSDTVPHSLSHDVYRKQPPSPQIPSSLQMSEPLYSSTTTNVQKPCSAFHPVTTQHNRYDGYQVNPPYNPRNYTPDEAQYVREQEKTRKINRNFSLTERDYPRSRRDFKTVDGANVPGFVRTGGNSITSGRNEEHLFTPLRNRAMSENNLCVETQHYQNHSSSSGVSRMRSSTLSDLDENTVSVGEIKKKKGPPPPRPPPPKWDQFHKRRASHHSLFSSTSHYSSPTASPPRPQPQTCTSRPPSVSEMSRQRSYSLPPRDMSESLHCCRQEYSVAPSSPAFTRRAFKPVALPPREMDYNTRREVHQPEPCTRIPIHPAAAPEEPRATHLKPTFPDHGVEWDRSSPHYPTHGTSRTPEVPSNSFSAGPLCPESYFSMNNFQLQPQQAGFPITAHKTQIIPSQGPEDGDLPLETDIDEICENEQAERKETVDRMEMQGFARPVMVLETDIDHTPEEAAPSAISIRGPRGSLVDSILEDEYGVSRKELLGELFPHSVNAEMSGDGWRGGYPISGGTLERPSRLNTTAPQVSRSTCYDISADNPQLLAKLREISERKEEDEELNYKKQLMESLRKKLTVLREAQRGLQEDIRANAQLGDEVESLVLAICKPNEVDKYRMFIGDLDKVTSLLLSLSGRLIRVESALDCVDPETGHQERLQLLEKKKQLLVQMGEAQELKEHVDRREQAVCRVLGCCLTPEQMRDYGHFVKMKAALLVEQRQLDDKIRLGEEQLRGLRESLGLGFGMAYGHY

Target ORF information:

RefSeq Version	XM_005172333.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio shroom family member 4 (shroom4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172333.4

ORF Insert Sequence:

ATGGAGACCG TAGAGCAGCT GGTGTCTTTC CAACACATCC ATGTGCAGCT GAACGGAGGC 
GCTCCATGGG GATTCACTCT AAAAGGAGGA CTGGAGCACG GCGAACCACT CATCATCACT 
AAAATAGAAG AGGGAGGAAA AGCAGCTCAG TGTAAGAAGT TGAGAGTGGG GGATGAACTG 
GTGAACATCA ACGGCTCTGC TCTCTACGGC TCACGGCAAG AAGCTCTCAT TCTCATCAAA 
GGTTCCTACA GGGTTCTGAA GATCATCGTC CGAAGGCGCA GTGTGCCAGT GATCCGCCCT 
CACTCTTGGC ACCTGGCAAA GCTGACAGAG GCACCTTGCA CTACGGGCAC TGGTGATCCA 
TCCGATGGCC CTCCGGCTAT GCAGCTTCAC TTTACACCTC TCAATGTACC TTGGCACTCC 
GGAGGTGACA ACAGTGAGCT GTCCATGCAG TGGGGCCATA TCTCCAGGCA CTACAGCACT 
GACCGCAGCA GCTCAATAGG GAGTATGGAG AGTCTGGAGA ATCCTCCTAA CCAGGGATAC 
TATGACAGTC AGCTCTCTCC CATTGATCCT GTCATCTTTA ACAACAAACG TGACTCTGCT 
TACAGCTCTT TCTCAGCCAG CTCGAACACA TCTGATTACA CAGTTCCTGT CAAACCTGAG 
GAGACCAATT CCATGGACAG TCTTCTTCAG GGTTTGGGTT CTTCCTGCAG GTATCCAGAC 
AGAGGCCAAC ACTCGGCCCC CACTGGCCAT GGAAACCAGC TGGAAGAACA AGAACACTCC 
AAGGTGCTGT CTGGCAGATC CGAGCCCAAA GTCCGACCAT CGTCCTACAG CTGTGAGGAG 
GAAAACTGTG CACCACCACA GCCACCAATG AGGAAGGAGA GTTTTAGGGC TACTAGAGGC 
CAAGCAACAG ATAAACGCTG TGTGTCTGCT CCTGTAGGCA TCCCAAATGT AAGCAGTTGC 
ACGGATGAAG ATCAATCCCA AATCCAGGAT GTTTTGACTG GCAGAGTTTA TTTGAACGGA 
ATACAAGACA GTGAGCCAGA GCGTAAAGGG TGCAGCATTC AACCTTATTA TACCTTGAAC 
TCAGACTCAG GTGGAGATGG CAAAGCAACT GACCATGAGA AAGAACACTT GGAGGACTAT 
TCAAAATCTG TGGCAGCAGC ATCTTCACCT CCTTCACAAA GGAATAGGGA TGAGAACTTT 
GATACCAAAC TCCGACCTGA ACATGATCTC CAGTCAGCAA TGAACCGACA TAGTGCACCT 
GAGAAACTTC TAGCTGCTCA GTTGTGTATG ATGGATGTTT CTGCTGACAA AAGTGATCAT 
AGTGCATCTC CAACTTGCCA GTGGTCACAG TCCCCTCTTT ATCTGAGTGA TTCCAGTCAA 
AATACTGAGC AGGGCAAGTG GGGAACGAGC AGATGCTCTA CACCTGGTTC GGTCGCAACT 
TCGGAAGTCG AAGATCCCAG ACTAGAGGAG GAATCTATTG ATGGACAGAA TCCATGGGGG 
CATCCTATCA GTTTGTCTGG GAGTCCCATT GGAAATGGAT TCTCTCAGAA CAGTGAGAAC 
AATGGAGGAG AAGTAATGCT GGATGATGGC CTTGGCCCAA AGCAGCCACA GAAACGTCAG 
TTTCGTTGCT CCAAGTCTCG TCGCAGAAGT GAACGTTTTG CCACGAACCT CAGAAATGAG 
ATCCAGAGGA AGAAGGCACA GTTGCAGAAG AGTAGAAACC CTTGCGGGGA AGAGACAGTG 
GAAGAAGAGG TTGGAGATTT CAACACTGAG GTTGTAGCTC CTCCAGAATA CCGTCAACAT 
AAACCCCAAA TCTTACCAAA ACCTGCCACC AATACTGTAC CTATACAAAT ATCTAAACCG 
GCAACTCAAA AAGTTTTTGA TGCCACACAA ACTGAAGAAT ATTCCAGTGC CAGGGTTGTG 
TGTTTCCAGA CATCTCAGAC TCAAACACAA GTCCCTCAAC ATGTCAGACA AGTGTGTGTA 
CATGTGGTAG AGGAGGTAGC ACCTGCAGGC AAACCACGTC GGTGGCGTTG GACGCCAGAA 
CGCAAACTTG AACTCGAAAA TGAATCATCT GAAAGTAAGA GGAATGAGAC TATTGGACAG 
ATGTGGTCTG GAAAAATGGA ATCAACAAGA AGTAGGACGA GTTCATCCAG CGGTCGTTTA 
GGTCGATCGG ATGACTGTGA CATCCTTCCC TTTGCAGATC GCAGGAGGTT TTTTGAGGAG 
ACTAGCAGAA AGTTGAGCCA GTCTGTAACA AATTTATCAA ACCTGACAAG TCGAAGTCAG 
AGACTAGACA AGCCGGGCAG AATGAACCAT CCATCATCAC CTGAACCTCA TGAGATTGCT 
GCTCACCTTG GCCGTAGGAG ATTCTCATAT CAAGATGTGC CCTACATCAA CTCACTGGAC 
AATGGGAGAC AGTTTATAAG TAACCAACAA GACCAGGAAA AAACGAGGCA GAGGCTGATT 
GAGAGAGAGC AGGAGAGGGA AAGAGAGCAG GAGAGACTTA AGGAGCAAGA GAAGCTTAGA 
GAACAAGAAA GAGAAAAGGA GAGGTTAAGG AAAGAGAGGG AAAATGAGCA ACAAAGGCTG 
AAGGACTGGG AGGAACGGCA GAGGCTGCTA CAGAGAGAAC AAGAATGGGA GTCTGTGAGA 
GAATCTGTTC ACTCTGAACA CAATACGAGC AGTGACACAG TTCCTCACTC TTTATCTCAT 
GACGTCTATC GGAAGCAGCC GCCTAGTCCT CAGATTCCTT CCTCTCTGCA AATGTCTGAA 
CCTTTATATA GCAGCACTAC CACCAACGTC CAAAAGCCTT GCTCAGCGTT CCACCCTGTG 
ACCACCCAGC ACAACCGGTA TGATGGCTAC CAAGTAAATC CACCCTACAA TCCCAGGAAC 
TACACCCCAG ATGAGGCCCA GTATGTTCGG GAACAGGAAA AAACTAGAAA GATCAACAGA 
AACTTCAGCT TAACAGAGAG GGACTACCCA AGGTCCAGGA GAGACTTCAA GACAGTAGAT 
GGTGCTAATG TCCCCGGCTT CGTGCGCACA GGAGGCAACA GCATCACAAG TGGACGCAAC 
GAGGAGCATC TTTTTACCCC TCTTAGAAAC CGTGCCATGT CAGAGAATAA CCTGTGTGTG 
GAGACACAAC ATTATCAAAA TCACAGCTCC AGCTCTGGCG TCAGCAGAAT GCGCTCGTCC 
ACTCTTAGTG ACCTGGATGA AAATACAGTA AGTGTCGGGG AGATCAAAAA GAAAAAAGGG 
CCTCCGCCGC CTCGTCCACC ACCACCAAAA TGGGATCAGT TCCACAAGAG ACGGGCGTCC 
CATCACAGCC TGTTCTCCTC TACATCACAC TACTCCTCTC CAACTGCTTC TCCACCTCGG 
CCACAGCCAC AGACGTGCAC ATCACGACCC CCCAGTGTCT CTGAAATGAG CCGTCAGCGC 
TCTTACAGTC TTCCTCCAAG AGACATGTCC GAGAGTCTTC ACTGCTGCCG TCAGGAGTAC 
TCCGTCGCTC CCTCCAGTCC TGCTTTTACA CGTCGAGCTT TTAAGCCTGT AGCCCTGCCG 
CCACGAGAGA TGGACTATAA CACGAGGAGA GAAGTTCACC AGCCTGAACC ATGCACCAGA 
ATCCCCATCC ATCCTGCTGC AGCACCAGAA GAGCCCAGAG CCACACATCT GAAGCCCACA 
TTCCCTGATC ATGGAGTGGA GTGGGATCGA TCCAGCCCTC ACTACCCCAC ACATGGCACA 
AGCAGGACTC CTGAAGTGCC CTCAAACAGT TTCTCAGCTG GGCCTTTATG TCCAGAGTCC 
TACTTCTCCA TGAACAACTT CCAACTGCAG CCCCAGCAAG CAGGCTTCCC CATAACAGCC 
CACAAAACCC AGATCATCCC CTCTCAGGGT CCTGAAGATG GAGACCTGCC TCTGGAGACG 
GACATCGATG AGATCTGTGA GAATGAGCAG GCTGAGAGGA AGGAGACGGT GGACCGGATG 
GAGATGCAGG GCTTTGCTCG ACCTGTGATG GTGTTGGAAA CCGACATTGA CCACACGCCT 
GAGGAAGCGG CTCCATCGGC CATAAGCATT AGAGGGCCGA GGGGCTCCCT GGTGGACTCC 
ATTTTGGAGG ATGAGTACGG TGTGTCTAGG AAGGAGCTGC TTGGAGAGTT GTTTCCACAC 
AGTGTGAATG CTGAGATGAG TGGGGATGGC TGGAGAGGAG GATATCCCAT CAGTGGAGGA 
ACACTGGAGA GACCCTCCAG ACTGAATACC ACAGCGCCAC AAGTATCACG ATCCACCTGT 
TATGACATAT CAGCAGACAA CCCACAGCTT CTCGCCAAGC TTAGAGAGAT CTCAGAGAGG 
AAAGAGGAAG ATGAGGAGCT CAATTATAAG AAGCAGCTGA TGGAGAGTCT GCGTAAAAAG 
CTGACTGTCC TGCGTGAGGC ACAGCGGGGT CTGCAGGAGG ACATTCGGGC CAATGCTCAG 
CTGGGAGATG AGGTGGAGAG TCTGGTGCTG GCCATCTGCA AGCCCAACGA GGTGGACAAG 
TATCGGATGT TCATCGGCGA CCTTGACAAG GTGACCAGTC TGCTGCTGTC GCTTTCCGGA 
AGGCTGATCC GAGTGGAAAG TGCCCTGGAC TGTGTGGACC CAGAAACTGG ACACCAGGAG 
AGGCTCCAAC TCCTGGAGAA GAAGAAGCAG CTGTTGGTTC AGATGGGAGA GGCGCAGGAG 
CTGAAGGAGC ACGTGGACCG GCGCGAGCAG GCCGTCTGCA GAGTGCTGGG ATGCTGCCTG 
ACCCCAGAAC AGATGCGGGA CTACGGTCAC TTTGTGAAGA TGAAGGCGGC GCTACTGGTA 
GAGCAGAGGC AACTGGATGA CAAGATACGG CTCGGCGAGG AGCAGCTCAG GGGACTTCGA 
GAGAGCCTCG GCCTGGGTTT TGGGATGGCG TATGGCCATT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shroom4 ( XM_005172333.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172390.1 Danio rerio shroom family member 4 (shroom4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa12970
Clone ID Related Accession (Same CDS sequence)	XM_005172333.4 , XM_005172333.3
Accession Version	XM_005172333.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein Shroom4 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172333.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACCG TAGAGCAGCT GGTGTCTTTC CAACACATCC ATGTGCAGCT GAACGGAGGC 
GCTCCATGGG GATTCACTCT AAAAGGAGGA CTGGAGCACG GCGAACCACT CATCATCACT 
AAAATAGAAG AGGGAGGAAA AGCAGCTCAG TGTAAGAAGT TGAGAGTGGG GGATGAACTG 
GTGAACATCA ACGGCTCTGC TCTCTACGGC TCACGGCAAG AAGCTCTCAT TCTCATCAAA 
GGTTCCTACA GGGTTCTGAA GATCATCGTC CGAAGGCGCA GTGTGCCAGT GATCCGCCCT 
CACTCTTGGC ACCTGGCAAA GCTGACAGAG GCACCTTGCA CTACGGGCAC TGGTGATCCA 
TCCGATGGCC CTCCGGCTAT GCAGCTTCAC TTTACACCTC TCAATGTACC TTGGCACTCC 
GGAGGTGACA ACAGTGAGCT GTCCATGCAG TGGGGCCATA TCTCCAGGCA CTACAGCACT 
GACCGCAGCA GCTCAATAGG GAGTATGGAG AGTCTGGAGA ATCCTCCTAA CCAGGGATAC 
TATGACAGTC AGCTCTCTCC CATTGATCCT GTCATCTTTA ACAACAAACG TGACTCTGCT 
TACAGCTCTT TCTCAGCCAG CTCGAACACA TCTGATTACA CAGTTCCTGT CAAACCTGAG 
GAGACCAATT CCATGGACAG TCTTCTTCAG GGTTTGGGTT CTTCCTGCAG GTATCCAGAC 
AGAGGCCAAC ACTCGGCCCC CACTGGCCAT GGAAACCAGC TGGAAGAACA AGAACACTCC 
AAGGTGCTGT CTGGCAGATC CGAGCCCAAA GTCCGACCAT CGTCCTACAG CTGTGAGGAG 
GAAAACTGTG CACCACCACA GCCACCAATG AGGAAGGAGA GTTTTAGGGC TACTAGAGGC 
CAAGCAACAG ATAAACGCTG TGTGTCTGCT CCTGTAGGCA TCCCAAATGT AAGCAGTTGC 
ACGGATGAAG ATCAATCCCA AATCCAGGAT GTTTTGACTG GCAGAGTTTA TTTGAACGGA 
ATACAAGACA GTGAGCCAGA GCGTAAAGGG TGCAGCATTC AACCTTATTA TACCTTGAAC 
TCAGACTCAG GTGGAGATGG CAAAGCAACT GACCATGAGA AAGAACACTT GGAGGACTAT 
TCAAAATCTG TGGCAGCAGC ATCTTCACCT CCTTCACAAA GGAATAGGGA TGAGAACTTT 
GATACCAAAC TCCGACCTGA ACATGATCTC CAGTCAGCAA TGAACCGACA TAGTGCACCT 
GAGAAACTTC TAGCTGCTCA GTTGTGTATG ATGGATGTTT CTGCTGACAA AAGTGATCAT 
AGTGCATCTC CAACTTGCCA GTGGTCACAG TCCCCTCTTT ATCTGAGTGA TTCCAGTCAA 
AATACTGAGC AGGGCAAGTG GGGAACGAGC AGATGCTCTA CACCTGGTTC GGTCGCAACT 
TCGGAAGTCG AAGATCCCAG ACTAGAGGAG GAATCTATTG ATGGACAGAA TCCATGGGGG 
CATCCTATCA GTTTGTCTGG GAGTCCCATT GGAAATGGAT TCTCTCAGAA CAGTGAGAAC 
AATGGAGGAG AAGTAATGCT GGATGATGGC CTTGGCCCAA AGCAGCCACA GAAACGTCAG 
TTTCGTTGCT CCAAGTCTCG TCGCAGAAGT GAACGTTTTG CCACGAACCT CAGAAATGAG 
ATCCAGAGGA AGAAGGCACA GTTGCAGAAG AGTAGAAACC CTTGCGGGGA AGAGACAGTG 
GAAGAAGAGG TTGGAGATTT CAACACTGAG GTTGTAGCTC CTCCAGAATA CCGTCAACAT 
AAACCCCAAA TCTTACCAAA ACCTGCCACC AATACTGTAC CTATACAAAT ATCTAAACCG 
GCAACTCAAA AAGTTTTTGA TGCCACACAA ACTGAAGAAT ATTCCAGTGC CAGGGTTGTG 
TGTTTCCAGA CATCTCAGAC TCAAACACAA GTCCCTCAAC ATGTCAGACA AGTGTGTGTA 
CATGTGGTAG AGGAGGTAGC ACCTGCAGGC AAACCACGTC GGTGGCGTTG GACGCCAGAA 
CGCAAACTTG AACTCGAAAA TGAATCATCT GAAAGTAAGA GGAATGAGAC TATTGGACAG 
ATGTGGTCTG GAAAAATGGA ATCAACAAGA AGTAGGACGA GTTCATCCAG CGGTCGTTTA 
GGTCGATCGG ATGACTGTGA CATCCTTCCC TTTGCAGATC GCAGGAGGTT TTTTGAGGAG 
ACTAGCAGAA AGTTGAGCCA GTCTGTAACA AATTTATCAA ACCTGACAAG TCGAAGTCAG 
AGACTAGACA AGCCGGGCAG AATGAACCAT CCATCATCAC CTGAACCTCA TGAGATTGCT 
GCTCACCTTG GCCGTAGGAG ATTCTCATAT CAAGATGTGC CCTACATCAA CTCACTGGAC 
AATGGGAGAC AGTTTATAAG TAACCAACAA GACCAGGAAA AAACGAGGCA GAGGCTGATT 
GAGAGAGAGC AGGAGAGGGA AAGAGAGCAG GAGAGACTTA AGGAGCAAGA GAAGCTTAGA 
GAACAAGAAA GAGAAAAGGA GAGGTTAAGG AAAGAGAGGG AAAATGAGCA ACAAAGGCTG 
AAGGACTGGG AGGAACGGCA GAGGCTGCTA CAGAGAGAAC AAGAATGGGA GTCTGTGAGA 
GAATCTGTTC ACTCTGAACA CAATACGAGC AGTGACACAG TTCCTCACTC TTTATCTCAT 
GACGTCTATC GGAAGCAGCC GCCTAGTCCT CAGATTCCTT CCTCTCTGCA AATGTCTGAA 
CCTTTATATA GCAGCACTAC CACCAACGTC CAAAAGCCTT GCTCAGCGTT CCACCCTGTG 
ACCACCCAGC ACAACCGGTA TGATGGCTAC CAAGTAAATC CACCCTACAA TCCCAGGAAC 
TACACCCCAG ATGAGGCCCA GTATGTTCGG GAACAGGAAA AAACTAGAAA GATCAACAGA 
AACTTCAGCT TAACAGAGAG GGACTACCCA AGGTCCAGGA GAGACTTCAA GACAGTAGAT 
GGTGCTAATG TCCCCGGCTT CGTGCGCACA GGAGGCAACA GCATCACAAG TGGACGCAAC 
GAGGAGCATC TTTTTACCCC TCTTAGAAAC CGTGCCATGT CAGAGAATAA CCTGTGTGTG 
GAGACACAAC ATTATCAAAA TCACAGCTCC AGCTCTGGCG TCAGCAGAAT GCGCTCGTCC 
ACTCTTAGTG ACCTGGATGA AAATACAGTA AGTGTCGGGG AGATCAAAAA GAAAAAAGGG 
CCTCCGCCGC CTCGTCCACC ACCACCAAAA TGGGATCAGT TCCACAAGAG ACGGGCGTCC 
CATCACAGCC TGTTCTCCTC TACATCACAC TACTCCTCTC CAACTGCTTC TCCACCTCGG 
CCACAGCCAC AGACGTGCAC ATCACGACCC CCCAGTGTCT CTGAAATGAG CCGTCAGCGC 
TCTTACAGTC TTCCTCCAAG AGACATGTCC GAGAGTCTTC ACTGCTGCCG TCAGGAGTAC 
TCCGTCGCTC CCTCCAGTCC TGCTTTTACA CGTCGAGCTT TTAAGCCTGT AGCCCTGCCG 
CCACGAGAGA TGGACTATAA CACGAGGAGA GAAGTTCACC AGCCTGAACC ATGCACCAGA 
ATCCCCATCC ATCCTGCTGC AGCACCAGAA GAGCCCAGAG CCACACATCT GAAGCCCACA 
TTCCCTGATC ATGGAGTGGA GTGGGATCGA TCCAGCCCTC ACTACCCCAC ACATGGCACA 
AGCAGGACTC CTGAAGTGCC CTCAAACAGT TTCTCAGCTG GGCCTTTATG TCCAGAGTCC 
TACTTCTCCA TGAACAACTT CCAACTGCAG CCCCAGCAAG CAGGCTTCCC CATAACAGCC 
CACAAAACCC AGATCATCCC CTCTCAGGGT CCTGAAGATG GAGACCTGCC TCTGGAGACG 
GACATCGATG AGATCTGTGA GAATGAGCAG GCTGAGAGGA AGGAGACGGT GGACCGGATG 
GAGATGCAGG GCTTTGCTCG ACCTGTGATG GTGTTGGAAA CCGACATTGA CCACACGCCT 
GAGGAAGCGG CTCCATCGGC CATAAGCATT AGAGGGCCGA GGGGCTCCCT GGTGGACTCC 
ATTTTGGAGG ATGAGTACGG TGTGTCTAGG AAGGAGCTGC TTGGAGAGTT GTTTCCACAC 
AGTGTGAATG CTGAGATGAG TGGGGATGGC TGGAGAGGAG GATATCCCAT CAGTGGAGGA 
ACACTGGAGA GACCCTCCAG ACTGAATACC ACAGCGCCAC AAGTATCACG ATCCACCTGT 
TATGACATAT CAGCAGACAA CCCACAGCTT CTCGCCAAGC TTAGAGAGAT CTCAGAGAGG 
AAAGAGGAAG ATGAGGAGCT CAATTATAAG AAGCAGCTGA TGGAGAGTCT GCGTAAAAAG 
CTGACTGTCC TGCGTGAGGC ACAGCGGGGT CTGCAGGAGG ACATTCGGGC CAATGCTCAG 
CTGGGAGATG AGGTGGAGAG TCTGGTGCTG GCCATCTGCA AGCCCAACGA GGTGGACAAG 
TATCGGATGT TCATCGGCGA CCTTGACAAG GTGACCAGTC TGCTGCTGTC GCTTTCCGGA 
AGGCTGATCC GAGTGGAAAG TGCCCTGGAC TGTGTGGACC CAGAAACTGG ACACCAGGAG 
AGGCTCCAAC TCCTGGAGAA GAAGAAGCAG CTGTTGGTTC AGATGGGAGA GGCGCAGGAG 
CTGAAGGAGC ACGTGGACCG GCGCGAGCAG GCCGTCTGCA GAGTGCTGGG ATGCTGCCTG 
ACCCCAGAAC AGATGCGGGA CTACGGTCAC TTTGTGAAGA TGAAGGCGGC GCTACTGGTA 
GAGCAGAGGC AACTGGATGA CAAGATACGG CTCGGCGAGG AGCAGCTCAG GGGACTTCGA 
GAGAGCCTCG GCCTGGGTTT TGGGATGGCG TATGGCCATT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172390.1
CDS	150..5054
Translation	METVEQLVSFQHIHVQLNGGAPWGFTLKGGLEHGEPLIITKIEEGGKAAQCKKLRVGDELVNINGSALYGSRQEALILIKGSYRVLKIIVRRRSVPVIRPHSWHLAKLTEAPCTTGTGDPSDGPPAMQLHFTPLNVPWHSGGDNSELSMQWGHISRHYSTDRSSSIGSMESLENPPNQGYYDSQLSPIDPVIFNNKRDSAYSSFSASSNTSDYTVPVKPEETNSMDSLLQGLGSSCRYPDRGQHSAPTGHGNQLEEQEHSKVLSGRSEPKVRPSSYSCEEENCAPPQPPMRKESFRATRGQATDKRCVSAPVGIPNVSSCTDEDQSQIQDVLTGRVYLNGIQDSEPERKGCSIQPYYTLNSDSGGDGKATDHEKEHLEDYSKSVAAASSPPSQRNRDENFDTKLRPEHDLQSAMNRHSAPEKLLAAQLCMMDVSADKSDHSASPTCQWSQSPLYLSDSSQNTEQGKWGTSRCSTPGSVATSEVEDPRLEEESIDGQNPWGHPISLSGSPIGNGFSQNSENNGGEVMLDDGLGPKQPQKRQFRCSKSRRRSERFATNLRNEIQRKKAQLQKSRNPCGEETVEEEVGDFNTEVVAPPEYRQHKPQILPKPATNTVPIQISKPATQKVFDATQTEEYSSARVVCFQTSQTQTQVPQHVRQVCVHVVEEVAPAGKPRRWRWTPERKLELENESSESKRNETIGQMWSGKMESTRSRTSSSSGRLGRSDDCDILPFADRRRFFEETSRKLSQSVTNLSNLTSRSQRLDKPGRMNHPSSPEPHEIAAHLGRRRFSYQDVPYINSLDNGRQFISNQQDQEKTRQRLIEREQEREREQERLKEQEKLREQEREKERLRKERENEQQRLKDWEERQRLLQREQEWESVRESVHSEHNTSSDTVPHSLSHDVYRKQPPSPQIPSSLQMSEPLYSSTTTNVQKPCSAFHPVTTQHNRYDGYQVNPPYNPRNYTPDEAQYVREQEKTRKINRNFSLTERDYPRSRRDFKTVDGANVPGFVRTGGNSITSGRNEEHLFTPLRNRAMSENNLCVETQHYQNHSSSSGVSRMRSSTLSDLDENTVSVGEIKKKKGPPPPRPPPPKWDQFHKRRASHHSLFSSTSHYSSPTASPPRPQPQTCTSRPPSVSEMSRQRSYSLPPRDMSESLHCCRQEYSVAPSSPAFTRRAFKPVALPPREMDYNTRREVHQPEPCTRIPIHPAAAPEEPRATHLKPTFPDHGVEWDRSSPHYPTHGTSRTPEVPSNSFSAGPLCPESYFSMNNFQLQPQQAGFPITAHKTQIIPSQGPEDGDLPLETDIDEICENEQAERKETVDRMEMQGFARPVMVLETDIDHTPEEAAPSAISIRGPRGSLVDSILEDEYGVSRKELLGELFPHSVNAEMSGDGWRGGYPISGGTLERPSRLNTTAPQVSRSTCYDISADNPQLLAKLREISERKEEDEELNYKKQLMESLRKKLTVLREAQRGLQEDIRANAQLGDEVESLVLAICKPNEVDKYRMFIGDLDKVTSLLLSLSGRLIRVESALDCVDPETGHQERLQLLEKKKQLLVQMGEAQELKEHVDRREQAVCRVLGCCLTPEQMRDYGHFVKMKAALLVEQRQLDDKIRLGEEQLRGLRESLGLGFGMAYGHY

Target ORF information:

RefSeq Version	XM_005172333.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio shroom family member 4 (shroom4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172333.3

ORF Insert Sequence:

ATGGAGACCG TAGAGCAGCT GGTGTCTTTC CAACACATCC ATGTGCAGCT GAACGGAGGC 
GCTCCATGGG GATTCACTCT AAAAGGAGGA CTGGAGCACG GCGAACCACT CATCATCACT 
AAAATAGAAG AGGGAGGAAA AGCAGCTCAG TGTAAGAAGT TGAGAGTGGG GGATGAACTG 
GTGAACATCA ACGGCTCTGC TCTCTACGGC TCACGGCAAG AAGCTCTCAT TCTCATCAAA 
GGTTCCTACA GGGTTCTGAA GATCATCGTC CGAAGGCGCA GTGTGCCAGT GATCCGCCCT 
CACTCTTGGC ACCTGGCAAA GCTGACAGAG GCACCTTGCA CTACGGGCAC TGGTGATCCA 
TCCGATGGCC CTCCGGCTAT GCAGCTTCAC TTTACACCTC TCAATGTACC TTGGCACTCC 
GGAGGTGACA ACAGTGAGCT GTCCATGCAG TGGGGCCATA TCTCCAGGCA CTACAGCACT 
GACCGCAGCA GCTCAATAGG GAGTATGGAG AGTCTGGAGA ATCCTCCTAA CCAGGGATAC 
TATGACAGTC AGCTCTCTCC CATTGATCCT GTCATCTTTA ACAACAAACG TGACTCTGCT 
TACAGCTCTT TCTCAGCCAG CTCGAACACA TCTGATTACA CAGTTCCTGT CAAACCTGAG 
GAGACCAATT CCATGGACAG TCTTCTTCAG GGTTTGGGTT CTTCCTGCAG GTATCCAGAC 
AGAGGCCAAC ACTCGGCCCC CACTGGCCAT GGAAACCAGC TGGAAGAACA AGAACACTCC 
AAGGTGCTGT CTGGCAGATC CGAGCCCAAA GTCCGACCAT CGTCCTACAG CTGTGAGGAG 
GAAAACTGTG CACCACCACA GCCACCAATG AGGAAGGAGA GTTTTAGGGC TACTAGAGGC 
CAAGCAACAG ATAAACGCTG TGTGTCTGCT CCTGTAGGCA TCCCAAATGT AAGCAGTTGC 
ACGGATGAAG ATCAATCCCA AATCCAGGAT GTTTTGACTG GCAGAGTTTA TTTGAACGGA 
ATACAAGACA GTGAGCCAGA GCGTAAAGGG TGCAGCATTC AACCTTATTA TACCTTGAAC 
TCAGACTCAG GTGGAGATGG CAAAGCAACT GACCATGAGA AAGAACACTT GGAGGACTAT 
TCAAAATCTG TGGCAGCAGC ATCTTCACCT CCTTCACAAA GGAATAGGGA TGAGAACTTT 
GATACCAAAC TCCGACCTGA ACATGATCTC CAGTCAGCAA TGAACCGACA TAGTGCACCT 
GAGAAACTTC TAGCTGCTCA GTTGTGTATG ATGGATGTTT CTGCTGACAA AAGTGATCAT 
AGTGCATCTC CAACTTGCCA GTGGTCACAG TCCCCTCTTT ATCTGAGTGA TTCCAGTCAA 
AATACTGAGC AGGGCAAGTG GGGAACGAGC AGATGCTCTA CACCTGGTTC GGTCGCAACT 
TCGGAAGTCG AAGATCCCAG ACTAGAGGAG GAATCTATTG ATGGACAGAA TCCATGGGGG 
CATCCTATCA GTTTGTCTGG GAGTCCCATT GGAAATGGAT TCTCTCAGAA CAGTGAGAAC 
AATGGAGGAG AAGTAATGCT GGATGATGGC CTTGGCCCAA AGCAGCCACA GAAACGTCAG 
TTTCGTTGCT CCAAGTCTCG TCGCAGAAGT GAACGTTTTG CCACGAACCT CAGAAATGAG 
ATCCAGAGGA AGAAGGCACA GTTGCAGAAG AGTAGAAACC CTTGCGGGGA AGAGACAGTG 
GAAGAAGAGG TTGGAGATTT CAACACTGAG GTTGTAGCTC CTCCAGAATA CCGTCAACAT 
AAACCCCAAA TCTTACCAAA ACCTGCCACC AATACTGTAC CTATACAAAT ATCTAAACCG 
GCAACTCAAA AAGTTTTTGA TGCCACACAA ACTGAAGAAT ATTCCAGTGC CAGGGTTGTG 
TGTTTCCAGA CATCTCAGAC TCAAACACAA GTCCCTCAAC ATGTCAGACA AGTGTGTGTA 
CATGTGGTAG AGGAGGTAGC ACCTGCAGGC AAACCACGTC GGTGGCGTTG GACGCCAGAA 
CGCAAACTTG AACTCGAAAA TGAATCATCT GAAAGTAAGA GGAATGAGAC TATTGGACAG 
ATGTGGTCTG GAAAAATGGA ATCAACAAGA AGTAGGACGA GTTCATCCAG CGGTCGTTTA 
GGTCGATCGG ATGACTGTGA CATCCTTCCC TTTGCAGATC GCAGGAGGTT TTTTGAGGAG 
ACTAGCAGAA AGTTGAGCCA GTCTGTAACA AATTTATCAA ACCTGACAAG TCGAAGTCAG 
AGACTAGACA AGCCGGGCAG AATGAACCAT CCATCATCAC CTGAACCTCA TGAGATTGCT 
GCTCACCTTG GCCGTAGGAG ATTCTCATAT CAAGATGTGC CCTACATCAA CTCACTGGAC 
AATGGGAGAC AGTTTATAAG TAACCAACAA GACCAGGAAA AAACGAGGCA GAGGCTGATT 
GAGAGAGAGC AGGAGAGGGA AAGAGAGCAG GAGAGACTTA AGGAGCAAGA GAAGCTTAGA 
GAACAAGAAA GAGAAAAGGA GAGGTTAAGG AAAGAGAGGG AAAATGAGCA ACAAAGGCTG 
AAGGACTGGG AGGAACGGCA GAGGCTGCTA CAGAGAGAAC AAGAATGGGA GTCTGTGAGA 
GAATCTGTTC ACTCTGAACA CAATACGAGC AGTGACACAG TTCCTCACTC TTTATCTCAT 
GACGTCTATC GGAAGCAGCC GCCTAGTCCT CAGATTCCTT CCTCTCTGCA AATGTCTGAA 
CCTTTATATA GCAGCACTAC CACCAACGTC CAAAAGCCTT GCTCAGCGTT CCACCCTGTG 
ACCACCCAGC ACAACCGGTA TGATGGCTAC CAAGTAAATC CACCCTACAA TCCCAGGAAC 
TACACCCCAG ATGAGGCCCA GTATGTTCGG GAACAGGAAA AAACTAGAAA GATCAACAGA 
AACTTCAGCT TAACAGAGAG GGACTACCCA AGGTCCAGGA GAGACTTCAA GACAGTAGAT 
GGTGCTAATG TCCCCGGCTT CGTGCGCACA GGAGGCAACA GCATCACAAG TGGACGCAAC 
GAGGAGCATC TTTTTACCCC TCTTAGAAAC CGTGCCATGT CAGAGAATAA CCTGTGTGTG 
GAGACACAAC ATTATCAAAA TCACAGCTCC AGCTCTGGCG TCAGCAGAAT GCGCTCGTCC 
ACTCTTAGTG ACCTGGATGA AAATACAGTA AGTGTCGGGG AGATCAAAAA GAAAAAAGGG 
CCTCCGCCGC CTCGTCCACC ACCACCAAAA TGGGATCAGT TCCACAAGAG ACGGGCGTCC 
CATCACAGCC TGTTCTCCTC TACATCACAC TACTCCTCTC CAACTGCTTC TCCACCTCGG 
CCACAGCCAC AGACGTGCAC ATCACGACCC CCCAGTGTCT CTGAAATGAG CCGTCAGCGC 
TCTTACAGTC TTCCTCCAAG AGACATGTCC GAGAGTCTTC ACTGCTGCCG TCAGGAGTAC 
TCCGTCGCTC CCTCCAGTCC TGCTTTTACA CGTCGAGCTT TTAAGCCTGT AGCCCTGCCG 
CCACGAGAGA TGGACTATAA CACGAGGAGA GAAGTTCACC AGCCTGAACC ATGCACCAGA 
ATCCCCATCC ATCCTGCTGC AGCACCAGAA GAGCCCAGAG CCACACATCT GAAGCCCACA 
TTCCCTGATC ATGGAGTGGA GTGGGATCGA TCCAGCCCTC ACTACCCCAC ACATGGCACA 
AGCAGGACTC CTGAAGTGCC CTCAAACAGT TTCTCAGCTG GGCCTTTATG TCCAGAGTCC 
TACTTCTCCA TGAACAACTT CCAACTGCAG CCCCAGCAAG CAGGCTTCCC CATAACAGCC 
CACAAAACCC AGATCATCCC CTCTCAGGGT CCTGAAGATG GAGACCTGCC TCTGGAGACG 
GACATCGATG AGATCTGTGA GAATGAGCAG GCTGAGAGGA AGGAGACGGT GGACCGGATG 
GAGATGCAGG GCTTTGCTCG ACCTGTGATG GTGTTGGAAA CCGACATTGA CCACACGCCT 
GAGGAAGCGG CTCCATCGGC CATAAGCATT AGAGGGCCGA GGGGCTCCCT GGTGGACTCC 
ATTTTGGAGG ATGAGTACGG TGTGTCTAGG AAGGAGCTGC TTGGAGAGTT GTTTCCACAC 
AGTGTGAATG CTGAGATGAG TGGGGATGGC TGGAGAGGAG GATATCCCAT CAGTGGAGGA 
ACACTGGAGA GACCCTCCAG ACTGAATACC ACAGCGCCAC AAGTATCACG ATCCACCTGT 
TATGACATAT CAGCAGACAA CCCACAGCTT CTCGCCAAGC TTAGAGAGAT CTCAGAGAGG 
AAAGAGGAAG ATGAGGAGCT CAATTATAAG AAGCAGCTGA TGGAGAGTCT GCGTAAAAAG 
CTGACTGTCC TGCGTGAGGC ACAGCGGGGT CTGCAGGAGG ACATTCGGGC CAATGCTCAG 
CTGGGAGATG AGGTGGAGAG TCTGGTGCTG GCCATCTGCA AGCCCAACGA GGTGGACAAG 
TATCGGATGT TCATCGGCGA CCTTGACAAG GTGACCAGTC TGCTGCTGTC GCTTTCCGGA 
AGGCTGATCC GAGTGGAAAG TGCCCTGGAC TGTGTGGACC CAGAAACTGG ACACCAGGAG 
AGGCTCCAAC TCCTGGAGAA GAAGAAGCAG CTGTTGGTTC AGATGGGAGA GGCGCAGGAG 
CTGAAGGAGC ACGTGGACCG GCGCGAGCAG GCCGTCTGCA GAGTGCTGGG ATGCTGCCTG 
ACCCCAGAAC AGATGCGGGA CTACGGTCAC TTTGTGAAGA TGAAGGCGGC GCTACTGGTA 
GAGCAGAGGC AACTGGATGA CAAGATACGG CTCGGCGAGG AGCAGCTCAG GGGACTTCGA 
GAGAGCCTCG GCCTGGGTTT TGGGATGGCG TATGGCCATT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shroom4 ( XM_682334.8 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_687426.5 Danio rerio shroom family member 4 (shroom4), transcript variant X3, mRNA.

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CloneID	ODa12972
Clone ID Related Accession (Same CDS sequence)	XM_682334.8 , XM_682334.9
Accession Version	XM_682334.8	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein Shroom4 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_682334.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCTTC ACTTTACACC TCTCAATGTA CCTTGGCACT CCGGAGGTGA CAACAGTGAG 
CTGTCCATGC AGTGGGGCCA TATCTCCAGG CACTACAGCA CTGACCGCAG CAGCTCAATA 
GGGAGTATGG AGAGTCTGGA GAATCCTCCT AACCAGGGAT ACTATGACAG TCAGCTCTCT 
CCCATTGATC CTGTCATCTT TAACAACAAA CGTGACTCTG CTTACAGCTC TTTCTCAGCC 
AGCTCGAACA CATCTGATTA CACAGTTCCT GTCAAACCTG AGGAGACCAA TTCCATGGAC 
AGTCTTCTTC AGGGTTTGGG TTCTTCCTGC AGGTATCCAG ACAGAGGCCA ACACTCGGCC 
CCCACTGGCC ATGGAAACCA GCTGGAAGAA CAAGAACACT CCAAGGTGCT GTCTGGCAGA 
TCCGAGCCCA AAGTCCGACC ATCGTCCTAC AGCTGTGAGG AGGAAAACTG TGCACCACCA 
CAGCCACCAA TGAGGAAGGA GAGTTTTAGG GCTACTAGAG GCCAAGCAAC AGATAAACGC 
TGTGTGTCTG CTCCTGTAGG CATCCCAAAT GTAAGCAGTT GCACGGATGA AGATCAATCC 
CAAATCCAGG ATGTTTTGAC TGGCAGAGTT TATTTGAACG GAATACAAGA CAGTGAGCCA 
GAGCGTAAAG GGTGCAGCAT TCAACCTTAT TATACCTTGA ACTCAGACTC AGGTGGAGAT 
GGCAAAGCAA CTGACCATGA GAAAGAACAC TTGGAGGACT ATTCAAAATC TGTGGCAGCA 
GCATCTTCAC CTCCTTCACA AAGGAATAGG GATGAGAACT TTGATACCAA ACTCCGACCT 
GAACATGATC TCCAGTCAGC AATGAACCGA CATAGTGCAC CTGAGAAACT TCTAGCTGCT 
CAGTTGTGTA TGATGGATGT TTCTGCTGAC AAAAGTGATC ATAGTGCATC TCCAACTTGC 
CAGTGGTCAC AGTCCCCTCT TTATCTGAGT GATTCCAGTC AAAATACTGA GCAGGGCAAG 
TGGGGAACGA GCAGATGCTC TACACCTGGT TCGGTCGCAA CTTCGGAAGT CGAAGATCCC 
AGACTAGAGG AGGAATCTAT TGATGGACAG AATCCATGGG GGCATCCTAT CAGTTTGTCT 
GGGAGTCCCA TTGGAAATGG ATTCTCTCAG AACAGTGAGA ACAATGGAGG AGAAGTAATG 
CTGGATGATG GCCTTGGCCC AAAGCAGCCA CAGAAACGTC AGTTTCGTTG CTCCAAGTCT 
CGTCGCAGAA GTGAACGTTT TGCCACGAAC CTCAGAAATG AGATCCAGAG GAAGAAGGCA 
CAGTTGCAGA AGAGTAGAAA CCCTTGCGGG GAAGAGACAG TGGAAGAAGA GGTTGGAGAT 
TTCAACACTG AGGTTGTAGC TCCTCCAGAA TACCGTCAAC ATAAACCCCA AATCTTACCA 
AAACCTGCCA CCAATACTGT ACCTATACAA ATATCTAAAC CGGCAACTCA AAAAGTTTTT 
GATGCCACAC AAACTGAAGA ATATTCCAGT GCCAGGGTTG TGTGTTTCCA GACATCTCAG 
ACTCAAACAC AAGTCCCTCA ACATGTCAGA CAAGTGTGTG TACATGTGGT AGAGGAGGTA 
GCACCTGCAG GCAAACCACG TCGGTGGCGT TGGACGCCAG AACGCAAACT TGAACTCGAA 
AATGAATCAT CTGAAAGTAA GAGGAATGAG ACTATTGGAC AGATGTGGTC TGGAAAAATG 
GAATCAACAA GAAGTAGGAC GAGTTCATCC AGCGGTCGTT TAGGTCGATC GGATGACTGT 
GACATCCTTC CCTTTGCAGA TCGCAGGAGG TTTTTTGAGG AGACTAGCAG AAAGTTGAGC 
CAGTCTGTAA CAAATTTATC AAACCTGACA AGTCGAAGTC AGAGACTAGA CAAGCCGGGC 
AGAATGAACC ATCCATCATC ACCTGAACCT CATGAGATTG CTGCTCACCT TGGCCGTAGG 
AGATTCTCAT ATCAAGATGT GCCCTACATC AACTCACTGG ACAATGGGAG ACAGTTTATA 
AGTAACCAAC AAGACCAGGA AAAAACGAGG CAGAGGCTGA TTGAGAGAGA GCAGGAGAGG 
GAAAGAGAGC AGGAGAGACT TAAGGAGCAA GAGAAGCTTA GAGAACAAGA AAGAGAAAAG 
GAGAGGTTAA GGAAAGAGAG GGAAAATGAG CAACAAAGGC TGAAGGACTG GGAGGAACGG 
CAGAGGCTGC TACAGAGAGA ACAAGAATGG GAGTCTGTGA GAGAATCTGT TCACTCTGAA 
CACAATACGA GCAGTGACAC AGTTCCTCAC TCTTTATCTC ATGACGTCTA TCGGAAGCAG 
CCGCCTAGTC CTCAGATTCC TTCCTCTCTG CAAATGTCTG AACCTTTATA TAGCAGCACT 
ACCACCAACG TCCAAAAGCC TTGCTCAGCG TTCCACCCTG TGACCACCCA GCACAACCGG 
TATGATGGCT ACCAAGTAAA TCCACCCTAC AATCCCAGGA ACTACACCCC AGATGAGGCC 
CAGTATGTTC GGGAACAGGA AAAAACTAGA AAGATCAACA GAAACTTCAG CTTAACAGAG 
AGGGACTACC CAAGGTCCAG GAGAGACTTC AAGACAGTAG ATGGTGCTAA TGTCCCCGGC 
TTCGTGCGCA CAGGAGGCAA CAGCATCACA AGTGGACGCA ACGAGGAGCA TCTTTTTACC 
CCTCTTAGAA ACCGTGCCAT GTCAGAGAAT AACCTGTGTG TGGAGACACA ACATTATCAA 
AATCACAGCT CCAGCTCTGG CGTCAGCAGA ATGCGCTCGT CCACTCTTAG TGACCTGGAT 
GAAAATACAG TAAGTGTCGG GGAGATCAAA AAGAAAAAAG GGCCTCCGCC GCCTCGTCCA 
CCACCACCAA AATGGGATCA GTTCCACAAG AGACGGGCGT CCCATCACAG CCTGTTCTCC 
TCTACATCAC ACTACTCCTC TCCAACTGCT TCTCCACCTC GGCCACAGCC ACAGACGTGC 
ACATCACGAC CCCCCAGTGT CTCTGAAATG AGCCGTCAGC GCTCTTACAG TCTTCCTCCA 
AGAGACATGT CCGAGAGTCT TCACTGCTGC CGTCAGGAGT ACTCCGTCGC TCCCTCCAGT 
CCTGCTTTTA CACGTCGAGC TTTTAAGCCT GTAGCCCTGC CGCCACGAGA GATGGACTAT 
AACACGAGGA GAGAAGTTCA CCAGCCTGAA CCATGCACCA GAATCCCCAT CCATCCTGCT 
GCAGCACCAG AAGAGCCCAG AGCCACACAT CTGAAGCCCA CATTCCCTGA TCATGGAGTG 
GAGTGGGATC GATCCAGCCC TCACTACCCC ACACATGGCA CAAGCAGGAC TCCTGAAGTG 
CCCTCAAACA GTTTCTCAGC TGGGCCTTTA TGTCCAGAGT CCTACTTCTC CATGAACAAC 
TTCCAACTGC AGCCCCAGCA AGCAGGCTTC CCCATAACAG CCCACAAAAC CCAGATCATC 
CCCTCTCAGG GTCCTGAAGA TGGAGACCTG CCTCTGGAGA CGGACATCGA TGAGATCTGT 
GAGAATGAGC AGGCTGAGAG GAAGGAGACG GTGGACCGGA TGGAGATGCA GGGCTTTGCT 
CGACCTGTGA TGGTGTTGGA AACCGACATT GACCACACGC CTGAGGAAGC GGCTCCATCG 
GCCATAAGCA TTAGAGGGCC GAGGGGCTCC CTGGTGGACT CCATTTTGGA GGATGAGTAC 
GGTGTGTCTA GGAAGGAGCT GCTTGGAGAG TTGTTTCCAC ACAGTGTGAA TGCTGAGATG 
AGTGGGGATG GCTGGAGAGG AGGATATCCC ATCAGTGGAG GAACACTGGA GAGACCCTCC 
AGACTGAATA CCACAGCGCC ACAAGTATCA CGATCCACCT GTTATGACAT ATCAGCAGAC 
AACCCACAGC TTCTCGCCAA GCTTAGAGAG ATCTCAGAGA GGAAAGAGGA AGATGAGGAG 
CTCAATTATA AGAAGCAGCT GATGGAGAGT CTGCGTAAAA AGCTGACTGT CCTGCGTGAG 
GCACAGCGGG GTCTGCAGGA GGACATTCGG GCCAATGCTC AGCTGGGAGA TGAGGTGGAG 
AGTCTGGTGC TGGCCATCTG CAAGCCCAAC GAGGTGGACA AGTATCGGAT GTTCATCGGC 
GACCTTGACA AGGTGACCAG TCTGCTGCTG TCGCTTTCCG GAAGGCTGAT CCGAGTGGAA 
AGTGCCCTGG ACTGTGTGGA CCCAGAAACT GGACACCAGG AGAGGCTCCA ACTCCTGGAG 
AAGAAGAAGC AGCTGTTGGT TCAGATGGGA GAGGCGCAGG AGCTGAAGGA GCACGTGGAC 
CGGCGCGAGC AGGCCGTCTG CAGAGTGCTG GGATGCTGCC TGACCCCAGA ACAGATGCGG 
GACTACGGTC ACTTTGTGAA GATGAAGGCG GCGCTACTGG TAGAGCAGAG GCAACTGGAT 
GACAAGATAC GGCTCGGCGA GGAGCAGCTC AGGGGACTTC GAGAGAGCCT CGGCCTGGGT 
TTTGGGATGG CGTATGGCCA TTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_687426.5
CDS	330..4856
Translation	MQLHFTPLNVPWHSGGDNSELSMQWGHISRHYSTDRSSSIGSMESLENPPNQGYYDSQLSPIDPVIFNNKRDSAYSSFSASSNTSDYTVPVKPEETNSMDSLLQGLGSSCRYPDRGQHSAPTGHGNQLEEQEHSKVLSGRSEPKVRPSSYSCEEENCAPPQPPMRKESFRATRGQATDKRCVSAPVGIPNVSSCTDEDQSQIQDVLTGRVYLNGIQDSEPERKGCSIQPYYTLNSDSGGDGKATDHEKEHLEDYSKSVAAASSPPSQRNRDENFDTKLRPEHDLQSAMNRHSAPEKLLAAQLCMMDVSADKSDHSASPTCQWSQSPLYLSDSSQNTEQGKWGTSRCSTPGSVATSEVEDPRLEEESIDGQNPWGHPISLSGSPIGNGFSQNSENNGGEVMLDDGLGPKQPQKRQFRCSKSRRRSERFATNLRNEIQRKKAQLQKSRNPCGEETVEEEVGDFNTEVVAPPEYRQHKPQILPKPATNTVPIQISKPATQKVFDATQTEEYSSARVVCFQTSQTQTQVPQHVRQVCVHVVEEVAPAGKPRRWRWTPERKLELENESSESKRNETIGQMWSGKMESTRSRTSSSSGRLGRSDDCDILPFADRRRFFEETSRKLSQSVTNLSNLTSRSQRLDKPGRMNHPSSPEPHEIAAHLGRRRFSYQDVPYINSLDNGRQFISNQQDQEKTRQRLIEREQEREREQERLKEQEKLREQEREKERLRKERENEQQRLKDWEERQRLLQREQEWESVRESVHSEHNTSSDTVPHSLSHDVYRKQPPSPQIPSSLQMSEPLYSSTTTNVQKPCSAFHPVTTQHNRYDGYQVNPPYNPRNYTPDEAQYVREQEKTRKINRNFSLTERDYPRSRRDFKTVDGANVPGFVRTGGNSITSGRNEEHLFTPLRNRAMSENNLCVETQHYQNHSSSSGVSRMRSSTLSDLDENTVSVGEIKKKKGPPPPRPPPPKWDQFHKRRASHHSLFSSTSHYSSPTASPPRPQPQTCTSRPPSVSEMSRQRSYSLPPRDMSESLHCCRQEYSVAPSSPAFTRRAFKPVALPPREMDYNTRREVHQPEPCTRIPIHPAAAPEEPRATHLKPTFPDHGVEWDRSSPHYPTHGTSRTPEVPSNSFSAGPLCPESYFSMNNFQLQPQQAGFPITAHKTQIIPSQGPEDGDLPLETDIDEICENEQAERKETVDRMEMQGFARPVMVLETDIDHTPEEAAPSAISIRGPRGSLVDSILEDEYGVSRKELLGELFPHSVNAEMSGDGWRGGYPISGGTLERPSRLNTTAPQVSRSTCYDISADNPQLLAKLREISERKEEDEELNYKKQLMESLRKKLTVLREAQRGLQEDIRANAQLGDEVESLVLAICKPNEVDKYRMFIGDLDKVTSLLLSLSGRLIRVESALDCVDPETGHQERLQLLEKKKQLLVQMGEAQELKEHVDRREQAVCRVLGCCLTPEQMRDYGHFVKMKAALLVEQRQLDDKIRLGEEQLRGLRESLGLGFGMAYGHY

Target ORF information:

RefSeq Version	XM_682334.8
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio shroom family member 4 (shroom4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_682334.8

ORF Insert Sequence:

ATGCAGCTTC ACTTTACACC TCTCAATGTA CCTTGGCACT CCGGAGGTGA CAACAGTGAG 
CTGTCCATGC AGTGGGGCCA TATCTCCAGG CACTACAGCA CTGACCGCAG CAGCTCAATA 
GGGAGTATGG AGAGTCTGGA GAATCCTCCT AACCAGGGAT ACTATGACAG TCAGCTCTCT 
CCCATTGATC CTGTCATCTT TAACAACAAA CGTGACTCTG CTTACAGCTC TTTCTCAGCC 
AGCTCGAACA CATCTGATTA CACAGTTCCT GTCAAACCTG AGGAGACCAA TTCCATGGAC 
AGTCTTCTTC AGGGTTTGGG TTCTTCCTGC AGGTATCCAG ACAGAGGCCA ACACTCGGCC 
CCCACTGGCC ATGGAAACCA GCTGGAAGAA CAAGAACACT CCAAGGTGCT GTCTGGCAGA 
TCCGAGCCCA AAGTCCGACC ATCGTCCTAC AGCTGTGAGG AGGAAAACTG TGCACCACCA 
CAGCCACCAA TGAGGAAGGA GAGTTTTAGG GCTACTAGAG GCCAAGCAAC AGATAAACGC 
TGTGTGTCTG CTCCTGTAGG CATCCCAAAT GTAAGCAGTT GCACGGATGA AGATCAATCC 
CAAATCCAGG ATGTTTTGAC TGGCAGAGTT TATTTGAACG GAATACAAGA CAGTGAGCCA 
GAGCGTAAAG GGTGCAGCAT TCAACCTTAT TATACCTTGA ACTCAGACTC AGGTGGAGAT 
GGCAAAGCAA CTGACCATGA GAAAGAACAC TTGGAGGACT ATTCAAAATC TGTGGCAGCA 
GCATCTTCAC CTCCTTCACA AAGGAATAGG GATGAGAACT TTGATACCAA ACTCCGACCT 
GAACATGATC TCCAGTCAGC AATGAACCGA CATAGTGCAC CTGAGAAACT TCTAGCTGCT 
CAGTTGTGTA TGATGGATGT TTCTGCTGAC AAAAGTGATC ATAGTGCATC TCCAACTTGC 
CAGTGGTCAC AGTCCCCTCT TTATCTGAGT GATTCCAGTC AAAATACTGA GCAGGGCAAG 
TGGGGAACGA GCAGATGCTC TACACCTGGT TCGGTCGCAA CTTCGGAAGT CGAAGATCCC 
AGACTAGAGG AGGAATCTAT TGATGGACAG AATCCATGGG GGCATCCTAT CAGTTTGTCT 
GGGAGTCCCA TTGGAAATGG ATTCTCTCAG AACAGTGAGA ACAATGGAGG AGAAGTAATG 
CTGGATGATG GCCTTGGCCC AAAGCAGCCA CAGAAACGTC AGTTTCGTTG CTCCAAGTCT 
CGTCGCAGAA GTGAACGTTT TGCCACGAAC CTCAGAAATG AGATCCAGAG GAAGAAGGCA 
CAGTTGCAGA AGAGTAGAAA CCCTTGCGGG GAAGAGACAG TGGAAGAAGA GGTTGGAGAT 
TTCAACACTG AGGTTGTAGC TCCTCCAGAA TACCGTCAAC ATAAACCCCA AATCTTACCA 
AAACCTGCCA CCAATACTGT ACCTATACAA ATATCTAAAC CGGCAACTCA AAAAGTTTTT 
GATGCCACAC AAACTGAAGA ATATTCCAGT GCCAGGGTTG TGTGTTTCCA GACATCTCAG 
ACTCAAACAC AAGTCCCTCA ACATGTCAGA CAAGTGTGTG TACATGTGGT AGAGGAGGTA 
GCACCTGCAG GCAAACCACG TCGGTGGCGT TGGACGCCAG AACGCAAACT TGAACTCGAA 
AATGAATCAT CTGAAAGTAA GAGGAATGAG ACTATTGGAC AGATGTGGTC TGGAAAAATG 
GAATCAACAA GAAGTAGGAC GAGTTCATCC AGCGGTCGTT TAGGTCGATC GGATGACTGT 
GACATCCTTC CCTTTGCAGA TCGCAGGAGG TTTTTTGAGG AGACTAGCAG AAAGTTGAGC 
CAGTCTGTAA CAAATTTATC AAACCTGACA AGTCGAAGTC AGAGACTAGA CAAGCCGGGC 
AGAATGAACC ATCCATCATC ACCTGAACCT CATGAGATTG CTGCTCACCT TGGCCGTAGG 
AGATTCTCAT ATCAAGATGT GCCCTACATC AACTCACTGG ACAATGGGAG ACAGTTTATA 
AGTAACCAAC AAGACCAGGA AAAAACGAGG CAGAGGCTGA TTGAGAGAGA GCAGGAGAGG 
GAAAGAGAGC AGGAGAGACT TAAGGAGCAA GAGAAGCTTA GAGAACAAGA AAGAGAAAAG 
GAGAGGTTAA GGAAAGAGAG GGAAAATGAG CAACAAAGGC TGAAGGACTG GGAGGAACGG 
CAGAGGCTGC TACAGAGAGA ACAAGAATGG GAGTCTGTGA GAGAATCTGT TCACTCTGAA 
CACAATACGA GCAGTGACAC AGTTCCTCAC TCTTTATCTC ATGACGTCTA TCGGAAGCAG 
CCGCCTAGTC CTCAGATTCC TTCCTCTCTG CAAATGTCTG AACCTTTATA TAGCAGCACT 
ACCACCAACG TCCAAAAGCC TTGCTCAGCG TTCCACCCTG TGACCACCCA GCACAACCGG 
TATGATGGCT ACCAAGTAAA TCCACCCTAC AATCCCAGGA ACTACACCCC AGATGAGGCC 
CAGTATGTTC GGGAACAGGA AAAAACTAGA AAGATCAACA GAAACTTCAG CTTAACAGAG 
AGGGACTACC CAAGGTCCAG GAGAGACTTC AAGACAGTAG ATGGTGCTAA TGTCCCCGGC 
TTCGTGCGCA CAGGAGGCAA CAGCATCACA AGTGGACGCA ACGAGGAGCA TCTTTTTACC 
CCTCTTAGAA ACCGTGCCAT GTCAGAGAAT AACCTGTGTG TGGAGACACA ACATTATCAA 
AATCACAGCT CCAGCTCTGG CGTCAGCAGA ATGCGCTCGT CCACTCTTAG TGACCTGGAT 
GAAAATACAG TAAGTGTCGG GGAGATCAAA AAGAAAAAAG GGCCTCCGCC GCCTCGTCCA 
CCACCACCAA AATGGGATCA GTTCCACAAG AGACGGGCGT CCCATCACAG CCTGTTCTCC 
TCTACATCAC ACTACTCCTC TCCAACTGCT TCTCCACCTC GGCCACAGCC ACAGACGTGC 
ACATCACGAC CCCCCAGTGT CTCTGAAATG AGCCGTCAGC GCTCTTACAG TCTTCCTCCA 
AGAGACATGT CCGAGAGTCT TCACTGCTGC CGTCAGGAGT ACTCCGTCGC TCCCTCCAGT 
CCTGCTTTTA CACGTCGAGC TTTTAAGCCT GTAGCCCTGC CGCCACGAGA GATGGACTAT 
AACACGAGGA GAGAAGTTCA CCAGCCTGAA CCATGCACCA GAATCCCCAT CCATCCTGCT 
GCAGCACCAG AAGAGCCCAG AGCCACACAT CTGAAGCCCA CATTCCCTGA TCATGGAGTG 
GAGTGGGATC GATCCAGCCC TCACTACCCC ACACATGGCA CAAGCAGGAC TCCTGAAGTG 
CCCTCAAACA GTTTCTCAGC TGGGCCTTTA TGTCCAGAGT CCTACTTCTC CATGAACAAC 
TTCCAACTGC AGCCCCAGCA AGCAGGCTTC CCCATAACAG CCCACAAAAC CCAGATCATC 
CCCTCTCAGG GTCCTGAAGA TGGAGACCTG CCTCTGGAGA CGGACATCGA TGAGATCTGT 
GAGAATGAGC AGGCTGAGAG GAAGGAGACG GTGGACCGGA TGGAGATGCA GGGCTTTGCT 
CGACCTGTGA TGGTGTTGGA AACCGACATT GACCACACGC CTGAGGAAGC GGCTCCATCG 
GCCATAAGCA TTAGAGGGCC GAGGGGCTCC CTGGTGGACT CCATTTTGGA GGATGAGTAC 
GGTGTGTCTA GGAAGGAGCT GCTTGGAGAG TTGTTTCCAC ACAGTGTGAA TGCTGAGATG 
AGTGGGGATG GCTGGAGAGG AGGATATCCC ATCAGTGGAG GAACACTGGA GAGACCCTCC 
AGACTGAATA CCACAGCGCC ACAAGTATCA CGATCCACCT GTTATGACAT ATCAGCAGAC 
AACCCACAGC TTCTCGCCAA GCTTAGAGAG ATCTCAGAGA GGAAAGAGGA AGATGAGGAG 
CTCAATTATA AGAAGCAGCT GATGGAGAGT CTGCGTAAAA AGCTGACTGT CCTGCGTGAG 
GCACAGCGGG GTCTGCAGGA GGACATTCGG GCCAATGCTC AGCTGGGAGA TGAGGTGGAG 
AGTCTGGTGC TGGCCATCTG CAAGCCCAAC GAGGTGGACA AGTATCGGAT GTTCATCGGC 
GACCTTGACA AGGTGACCAG TCTGCTGCTG TCGCTTTCCG GAAGGCTGAT CCGAGTGGAA 
AGTGCCCTGG ACTGTGTGGA CCCAGAAACT GGACACCAGG AGAGGCTCCA ACTCCTGGAG 
AAGAAGAAGC AGCTGTTGGT TCAGATGGGA GAGGCGCAGG AGCTGAAGGA GCACGTGGAC 
CGGCGCGAGC AGGCCGTCTG CAGAGTGCTG GGATGCTGCC TGACCCCAGA ACAGATGCGG 
GACTACGGTC ACTTTGTGAA GATGAAGGCG GCGCTACTGG TAGAGCAGAG GCAACTGGAT 
GACAAGATAC GGCTCGGCGA GGAGCAGCTC AGGGGACTTC GAGAGAGCCT CGGCCTGGGT 
TTTGGGATGG CGTATGGCCA TTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shroom4 ( XM_682334.9 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_687426.5 Danio rerio shroom family member 4 (shroom4), transcript variant X2, mRNA.

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CloneID	ODa12972
Clone ID Related Accession (Same CDS sequence)	XM_682334.8 , XM_682334.9
Accession Version	XM_682334.9 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein Shroom4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_682334.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCTTC ACTTTACACC TCTCAATGTA CCTTGGCACT CCGGAGGTGA CAACAGTGAG 
CTGTCCATGC AGTGGGGCCA TATCTCCAGG CACTACAGCA CTGACCGCAG CAGCTCAATA 
GGGAGTATGG AGAGTCTGGA GAATCCTCCT AACCAGGGAT ACTATGACAG TCAGCTCTCT 
CCCATTGATC CTGTCATCTT TAACAACAAA CGTGACTCTG CTTACAGCTC TTTCTCAGCC 
AGCTCGAACA CATCTGATTA CACAGTTCCT GTCAAACCTG AGGAGACCAA TTCCATGGAC 
AGTCTTCTTC AGGGTTTGGG TTCTTCCTGC AGGTATCCAG ACAGAGGCCA ACACTCGGCC 
CCCACTGGCC ATGGAAACCA GCTGGAAGAA CAAGAACACT CCAAGGTGCT GTCTGGCAGA 
TCCGAGCCCA AAGTCCGACC ATCGTCCTAC AGCTGTGAGG AGGAAAACTG TGCACCACCA 
CAGCCACCAA TGAGGAAGGA GAGTTTTAGG GCTACTAGAG GCCAAGCAAC AGATAAACGC 
TGTGTGTCTG CTCCTGTAGG CATCCCAAAT GTAAGCAGTT GCACGGATGA AGATCAATCC 
CAAATCCAGG ATGTTTTGAC TGGCAGAGTT TATTTGAACG GAATACAAGA CAGTGAGCCA 
GAGCGTAAAG GGTGCAGCAT TCAACCTTAT TATACCTTGA ACTCAGACTC AGGTGGAGAT 
GGCAAAGCAA CTGACCATGA GAAAGAACAC TTGGAGGACT ATTCAAAATC TGTGGCAGCA 
GCATCTTCAC CTCCTTCACA AAGGAATAGG GATGAGAACT TTGATACCAA ACTCCGACCT 
GAACATGATC TCCAGTCAGC AATGAACCGA CATAGTGCAC CTGAGAAACT TCTAGCTGCT 
CAGTTGTGTA TGATGGATGT TTCTGCTGAC AAAAGTGATC ATAGTGCATC TCCAACTTGC 
CAGTGGTCAC AGTCCCCTCT TTATCTGAGT GATTCCAGTC AAAATACTGA GCAGGGCAAG 
TGGGGAACGA GCAGATGCTC TACACCTGGT TCGGTCGCAA CTTCGGAAGT CGAAGATCCC 
AGACTAGAGG AGGAATCTAT TGATGGACAG AATCCATGGG GGCATCCTAT CAGTTTGTCT 
GGGAGTCCCA TTGGAAATGG ATTCTCTCAG AACAGTGAGA ACAATGGAGG AGAAGTAATG 
CTGGATGATG GCCTTGGCCC AAAGCAGCCA CAGAAACGTC AGTTTCGTTG CTCCAAGTCT 
CGTCGCAGAA GTGAACGTTT TGCCACGAAC CTCAGAAATG AGATCCAGAG GAAGAAGGCA 
CAGTTGCAGA AGAGTAGAAA CCCTTGCGGG GAAGAGACAG TGGAAGAAGA GGTTGGAGAT 
TTCAACACTG AGGTTGTAGC TCCTCCAGAA TACCGTCAAC ATAAACCCCA AATCTTACCA 
AAACCTGCCA CCAATACTGT ACCTATACAA ATATCTAAAC CGGCAACTCA AAAAGTTTTT 
GATGCCACAC AAACTGAAGA ATATTCCAGT GCCAGGGTTG TGTGTTTCCA GACATCTCAG 
ACTCAAACAC AAGTCCCTCA ACATGTCAGA CAAGTGTGTG TACATGTGGT AGAGGAGGTA 
GCACCTGCAG GCAAACCACG TCGGTGGCGT TGGACGCCAG AACGCAAACT TGAACTCGAA 
AATGAATCAT CTGAAAGTAA GAGGAATGAG ACTATTGGAC AGATGTGGTC TGGAAAAATG 
GAATCAACAA GAAGTAGGAC GAGTTCATCC AGCGGTCGTT TAGGTCGATC GGATGACTGT 
GACATCCTTC CCTTTGCAGA TCGCAGGAGG TTTTTTGAGG AGACTAGCAG AAAGTTGAGC 
CAGTCTGTAA CAAATTTATC AAACCTGACA AGTCGAAGTC AGAGACTAGA CAAGCCGGGC 
AGAATGAACC ATCCATCATC ACCTGAACCT CATGAGATTG CTGCTCACCT TGGCCGTAGG 
AGATTCTCAT ATCAAGATGT GCCCTACATC AACTCACTGG ACAATGGGAG ACAGTTTATA 
AGTAACCAAC AAGACCAGGA AAAAACGAGG CAGAGGCTGA TTGAGAGAGA GCAGGAGAGG 
GAAAGAGAGC AGGAGAGACT TAAGGAGCAA GAGAAGCTTA GAGAACAAGA AAGAGAAAAG 
GAGAGGTTAA GGAAAGAGAG GGAAAATGAG CAACAAAGGC TGAAGGACTG GGAGGAACGG 
CAGAGGCTGC TACAGAGAGA ACAAGAATGG GAGTCTGTGA GAGAATCTGT TCACTCTGAA 
CACAATACGA GCAGTGACAC AGTTCCTCAC TCTTTATCTC ATGACGTCTA TCGGAAGCAG 
CCGCCTAGTC CTCAGATTCC TTCCTCTCTG CAAATGTCTG AACCTTTATA TAGCAGCACT 
ACCACCAACG TCCAAAAGCC TTGCTCAGCG TTCCACCCTG TGACCACCCA GCACAACCGG 
TATGATGGCT ACCAAGTAAA TCCACCCTAC AATCCCAGGA ACTACACCCC AGATGAGGCC 
CAGTATGTTC GGGAACAGGA AAAAACTAGA AAGATCAACA GAAACTTCAG CTTAACAGAG 
AGGGACTACC CAAGGTCCAG GAGAGACTTC AAGACAGTAG ATGGTGCTAA TGTCCCCGGC 
TTCGTGCGCA CAGGAGGCAA CAGCATCACA AGTGGACGCA ACGAGGAGCA TCTTTTTACC 
CCTCTTAGAA ACCGTGCCAT GTCAGAGAAT AACCTGTGTG TGGAGACACA ACATTATCAA 
AATCACAGCT CCAGCTCTGG CGTCAGCAGA ATGCGCTCGT CCACTCTTAG TGACCTGGAT 
GAAAATACAG TAAGTGTCGG GGAGATCAAA AAGAAAAAAG GGCCTCCGCC GCCTCGTCCA 
CCACCACCAA AATGGGATCA GTTCCACAAG AGACGGGCGT CCCATCACAG CCTGTTCTCC 
TCTACATCAC ACTACTCCTC TCCAACTGCT TCTCCACCTC GGCCACAGCC ACAGACGTGC 
ACATCACGAC CCCCCAGTGT CTCTGAAATG AGCCGTCAGC GCTCTTACAG TCTTCCTCCA 
AGAGACATGT CCGAGAGTCT TCACTGCTGC CGTCAGGAGT ACTCCGTCGC TCCCTCCAGT 
CCTGCTTTTA CACGTCGAGC TTTTAAGCCT GTAGCCCTGC CGCCACGAGA GATGGACTAT 
AACACGAGGA GAGAAGTTCA CCAGCCTGAA CCATGCACCA GAATCCCCAT CCATCCTGCT 
GCAGCACCAG AAGAGCCCAG AGCCACACAT CTGAAGCCCA CATTCCCTGA TCATGGAGTG 
GAGTGGGATC GATCCAGCCC TCACTACCCC ACACATGGCA CAAGCAGGAC TCCTGAAGTG 
CCCTCAAACA GTTTCTCAGC TGGGCCTTTA TGTCCAGAGT CCTACTTCTC CATGAACAAC 
TTCCAACTGC AGCCCCAGCA AGCAGGCTTC CCCATAACAG CCCACAAAAC CCAGATCATC 
CCCTCTCAGG GTCCTGAAGA TGGAGACCTG CCTCTGGAGA CGGACATCGA TGAGATCTGT 
GAGAATGAGC AGGCTGAGAG GAAGGAGACG GTGGACCGGA TGGAGATGCA GGGCTTTGCT 
CGACCTGTGA TGGTGTTGGA AACCGACATT GACCACACGC CTGAGGAAGC GGCTCCATCG 
GCCATAAGCA TTAGAGGGCC GAGGGGCTCC CTGGTGGACT CCATTTTGGA GGATGAGTAC 
GGTGTGTCTA GGAAGGAGCT GCTTGGAGAG TTGTTTCCAC ACAGTGTGAA TGCTGAGATG 
AGTGGGGATG GCTGGAGAGG AGGATATCCC ATCAGTGGAG GAACACTGGA GAGACCCTCC 
AGACTGAATA CCACAGCGCC ACAAGTATCA CGATCCACCT GTTATGACAT ATCAGCAGAC 
AACCCACAGC TTCTCGCCAA GCTTAGAGAG ATCTCAGAGA GGAAAGAGGA AGATGAGGAG 
CTCAATTATA AGAAGCAGCT GATGGAGAGT CTGCGTAAAA AGCTGACTGT CCTGCGTGAG 
GCACAGCGGG GTCTGCAGGA GGACATTCGG GCCAATGCTC AGCTGGGAGA TGAGGTGGAG 
AGTCTGGTGC TGGCCATCTG CAAGCCCAAC GAGGTGGACA AGTATCGGAT GTTCATCGGC 
GACCTTGACA AGGTGACCAG TCTGCTGCTG TCGCTTTCCG GAAGGCTGAT CCGAGTGGAA 
AGTGCCCTGG ACTGTGTGGA CCCAGAAACT GGACACCAGG AGAGGCTCCA ACTCCTGGAG 
AAGAAGAAGC AGCTGTTGGT TCAGATGGGA GAGGCGCAGG AGCTGAAGGA GCACGTGGAC 
CGGCGCGAGC AGGCCGTCTG CAGAGTGCTG GGATGCTGCC TGACCCCAGA ACAGATGCGG 
GACTACGGTC ACTTTGTGAA GATGAAGGCG GCGCTACTGG TAGAGCAGAG GCAACTGGAT 
GACAAGATAC GGCTCGGCGA GGAGCAGCTC AGGGGACTTC GAGAGAGCCT CGGCCTGGGT 
TTTGGGATGG CGTATGGCCA TTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_687426.5
CDS	370..4896
Translation	MQLHFTPLNVPWHSGGDNSELSMQWGHISRHYSTDRSSSIGSMESLENPPNQGYYDSQLSPIDPVIFNNKRDSAYSSFSASSNTSDYTVPVKPEETNSMDSLLQGLGSSCRYPDRGQHSAPTGHGNQLEEQEHSKVLSGRSEPKVRPSSYSCEEENCAPPQPPMRKESFRATRGQATDKRCVSAPVGIPNVSSCTDEDQSQIQDVLTGRVYLNGIQDSEPERKGCSIQPYYTLNSDSGGDGKATDHEKEHLEDYSKSVAAASSPPSQRNRDENFDTKLRPEHDLQSAMNRHSAPEKLLAAQLCMMDVSADKSDHSASPTCQWSQSPLYLSDSSQNTEQGKWGTSRCSTPGSVATSEVEDPRLEEESIDGQNPWGHPISLSGSPIGNGFSQNSENNGGEVMLDDGLGPKQPQKRQFRCSKSRRRSERFATNLRNEIQRKKAQLQKSRNPCGEETVEEEVGDFNTEVVAPPEYRQHKPQILPKPATNTVPIQISKPATQKVFDATQTEEYSSARVVCFQTSQTQTQVPQHVRQVCVHVVEEVAPAGKPRRWRWTPERKLELENESSESKRNETIGQMWSGKMESTRSRTSSSSGRLGRSDDCDILPFADRRRFFEETSRKLSQSVTNLSNLTSRSQRLDKPGRMNHPSSPEPHEIAAHLGRRRFSYQDVPYINSLDNGRQFISNQQDQEKTRQRLIEREQEREREQERLKEQEKLREQEREKERLRKERENEQQRLKDWEERQRLLQREQEWESVRESVHSEHNTSSDTVPHSLSHDVYRKQPPSPQIPSSLQMSEPLYSSTTTNVQKPCSAFHPVTTQHNRYDGYQVNPPYNPRNYTPDEAQYVREQEKTRKINRNFSLTERDYPRSRRDFKTVDGANVPGFVRTGGNSITSGRNEEHLFTPLRNRAMSENNLCVETQHYQNHSSSSGVSRMRSSTLSDLDENTVSVGEIKKKKGPPPPRPPPPKWDQFHKRRASHHSLFSSTSHYSSPTASPPRPQPQTCTSRPPSVSEMSRQRSYSLPPRDMSESLHCCRQEYSVAPSSPAFTRRAFKPVALPPREMDYNTRREVHQPEPCTRIPIHPAAAPEEPRATHLKPTFPDHGVEWDRSSPHYPTHGTSRTPEVPSNSFSAGPLCPESYFSMNNFQLQPQQAGFPITAHKTQIIPSQGPEDGDLPLETDIDEICENEQAERKETVDRMEMQGFARPVMVLETDIDHTPEEAAPSAISIRGPRGSLVDSILEDEYGVSRKELLGELFPHSVNAEMSGDGWRGGYPISGGTLERPSRLNTTAPQVSRSTCYDISADNPQLLAKLREISERKEEDEELNYKKQLMESLRKKLTVLREAQRGLQEDIRANAQLGDEVESLVLAICKPNEVDKYRMFIGDLDKVTSLLLSLSGRLIRVESALDCVDPETGHQERLQLLEKKKQLLVQMGEAQELKEHVDRREQAVCRVLGCCLTPEQMRDYGHFVKMKAALLVEQRQLDDKIRLGEEQLRGLRESLGLGFGMAYGHY

Target ORF information:

RefSeq Version	XM_682334.9
Organism	Danio rerio(zebrafish)
Definition	Danio rerio shroom family member 4 (shroom4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_682334.9

ORF Insert Sequence:

ATGCAGCTTC ACTTTACACC TCTCAATGTA CCTTGGCACT CCGGAGGTGA CAACAGTGAG 
CTGTCCATGC AGTGGGGCCA TATCTCCAGG CACTACAGCA CTGACCGCAG CAGCTCAATA 
GGGAGTATGG AGAGTCTGGA GAATCCTCCT AACCAGGGAT ACTATGACAG TCAGCTCTCT 
CCCATTGATC CTGTCATCTT TAACAACAAA CGTGACTCTG CTTACAGCTC TTTCTCAGCC 
AGCTCGAACA CATCTGATTA CACAGTTCCT GTCAAACCTG AGGAGACCAA TTCCATGGAC 
AGTCTTCTTC AGGGTTTGGG TTCTTCCTGC AGGTATCCAG ACAGAGGCCA ACACTCGGCC 
CCCACTGGCC ATGGAAACCA GCTGGAAGAA CAAGAACACT CCAAGGTGCT GTCTGGCAGA 
TCCGAGCCCA AAGTCCGACC ATCGTCCTAC AGCTGTGAGG AGGAAAACTG TGCACCACCA 
CAGCCACCAA TGAGGAAGGA GAGTTTTAGG GCTACTAGAG GCCAAGCAAC AGATAAACGC 
TGTGTGTCTG CTCCTGTAGG CATCCCAAAT GTAAGCAGTT GCACGGATGA AGATCAATCC 
CAAATCCAGG ATGTTTTGAC TGGCAGAGTT TATTTGAACG GAATACAAGA CAGTGAGCCA 
GAGCGTAAAG GGTGCAGCAT TCAACCTTAT TATACCTTGA ACTCAGACTC AGGTGGAGAT 
GGCAAAGCAA CTGACCATGA GAAAGAACAC TTGGAGGACT ATTCAAAATC TGTGGCAGCA 
GCATCTTCAC CTCCTTCACA AAGGAATAGG GATGAGAACT TTGATACCAA ACTCCGACCT 
GAACATGATC TCCAGTCAGC AATGAACCGA CATAGTGCAC CTGAGAAACT TCTAGCTGCT 
CAGTTGTGTA TGATGGATGT TTCTGCTGAC AAAAGTGATC ATAGTGCATC TCCAACTTGC 
CAGTGGTCAC AGTCCCCTCT TTATCTGAGT GATTCCAGTC AAAATACTGA GCAGGGCAAG 
TGGGGAACGA GCAGATGCTC TACACCTGGT TCGGTCGCAA CTTCGGAAGT CGAAGATCCC 
AGACTAGAGG AGGAATCTAT TGATGGACAG AATCCATGGG GGCATCCTAT CAGTTTGTCT 
GGGAGTCCCA TTGGAAATGG ATTCTCTCAG AACAGTGAGA ACAATGGAGG AGAAGTAATG 
CTGGATGATG GCCTTGGCCC AAAGCAGCCA CAGAAACGTC AGTTTCGTTG CTCCAAGTCT 
CGTCGCAGAA GTGAACGTTT TGCCACGAAC CTCAGAAATG AGATCCAGAG GAAGAAGGCA 
CAGTTGCAGA AGAGTAGAAA CCCTTGCGGG GAAGAGACAG TGGAAGAAGA GGTTGGAGAT 
TTCAACACTG AGGTTGTAGC TCCTCCAGAA TACCGTCAAC ATAAACCCCA AATCTTACCA 
AAACCTGCCA CCAATACTGT ACCTATACAA ATATCTAAAC CGGCAACTCA AAAAGTTTTT 
GATGCCACAC AAACTGAAGA ATATTCCAGT GCCAGGGTTG TGTGTTTCCA GACATCTCAG 
ACTCAAACAC AAGTCCCTCA ACATGTCAGA CAAGTGTGTG TACATGTGGT AGAGGAGGTA 
GCACCTGCAG GCAAACCACG TCGGTGGCGT TGGACGCCAG AACGCAAACT TGAACTCGAA 
AATGAATCAT CTGAAAGTAA GAGGAATGAG ACTATTGGAC AGATGTGGTC TGGAAAAATG 
GAATCAACAA GAAGTAGGAC GAGTTCATCC AGCGGTCGTT TAGGTCGATC GGATGACTGT 
GACATCCTTC CCTTTGCAGA TCGCAGGAGG TTTTTTGAGG AGACTAGCAG AAAGTTGAGC 
CAGTCTGTAA CAAATTTATC AAACCTGACA AGTCGAAGTC AGAGACTAGA CAAGCCGGGC 
AGAATGAACC ATCCATCATC ACCTGAACCT CATGAGATTG CTGCTCACCT TGGCCGTAGG 
AGATTCTCAT ATCAAGATGT GCCCTACATC AACTCACTGG ACAATGGGAG ACAGTTTATA 
AGTAACCAAC AAGACCAGGA AAAAACGAGG CAGAGGCTGA TTGAGAGAGA GCAGGAGAGG 
GAAAGAGAGC AGGAGAGACT TAAGGAGCAA GAGAAGCTTA GAGAACAAGA AAGAGAAAAG 
GAGAGGTTAA GGAAAGAGAG GGAAAATGAG CAACAAAGGC TGAAGGACTG GGAGGAACGG 
CAGAGGCTGC TACAGAGAGA ACAAGAATGG GAGTCTGTGA GAGAATCTGT TCACTCTGAA 
CACAATACGA GCAGTGACAC AGTTCCTCAC TCTTTATCTC ATGACGTCTA TCGGAAGCAG 
CCGCCTAGTC CTCAGATTCC TTCCTCTCTG CAAATGTCTG AACCTTTATA TAGCAGCACT 
ACCACCAACG TCCAAAAGCC TTGCTCAGCG TTCCACCCTG TGACCACCCA GCACAACCGG 
TATGATGGCT ACCAAGTAAA TCCACCCTAC AATCCCAGGA ACTACACCCC AGATGAGGCC 
CAGTATGTTC GGGAACAGGA AAAAACTAGA AAGATCAACA GAAACTTCAG CTTAACAGAG 
AGGGACTACC CAAGGTCCAG GAGAGACTTC AAGACAGTAG ATGGTGCTAA TGTCCCCGGC 
TTCGTGCGCA CAGGAGGCAA CAGCATCACA AGTGGACGCA ACGAGGAGCA TCTTTTTACC 
CCTCTTAGAA ACCGTGCCAT GTCAGAGAAT AACCTGTGTG TGGAGACACA ACATTATCAA 
AATCACAGCT CCAGCTCTGG CGTCAGCAGA ATGCGCTCGT CCACTCTTAG TGACCTGGAT 
GAAAATACAG TAAGTGTCGG GGAGATCAAA AAGAAAAAAG GGCCTCCGCC GCCTCGTCCA 
CCACCACCAA AATGGGATCA GTTCCACAAG AGACGGGCGT CCCATCACAG CCTGTTCTCC 
TCTACATCAC ACTACTCCTC TCCAACTGCT TCTCCACCTC GGCCACAGCC ACAGACGTGC 
ACATCACGAC CCCCCAGTGT CTCTGAAATG AGCCGTCAGC GCTCTTACAG TCTTCCTCCA 
AGAGACATGT CCGAGAGTCT TCACTGCTGC CGTCAGGAGT ACTCCGTCGC TCCCTCCAGT 
CCTGCTTTTA CACGTCGAGC TTTTAAGCCT GTAGCCCTGC CGCCACGAGA GATGGACTAT 
AACACGAGGA GAGAAGTTCA CCAGCCTGAA CCATGCACCA GAATCCCCAT CCATCCTGCT 
GCAGCACCAG AAGAGCCCAG AGCCACACAT CTGAAGCCCA CATTCCCTGA TCATGGAGTG 
GAGTGGGATC GATCCAGCCC TCACTACCCC ACACATGGCA CAAGCAGGAC TCCTGAAGTG 
CCCTCAAACA GTTTCTCAGC TGGGCCTTTA TGTCCAGAGT CCTACTTCTC CATGAACAAC 
TTCCAACTGC AGCCCCAGCA AGCAGGCTTC CCCATAACAG CCCACAAAAC CCAGATCATC 
CCCTCTCAGG GTCCTGAAGA TGGAGACCTG CCTCTGGAGA CGGACATCGA TGAGATCTGT 
GAGAATGAGC AGGCTGAGAG GAAGGAGACG GTGGACCGGA TGGAGATGCA GGGCTTTGCT 
CGACCTGTGA TGGTGTTGGA AACCGACATT GACCACACGC CTGAGGAAGC GGCTCCATCG 
GCCATAAGCA TTAGAGGGCC GAGGGGCTCC CTGGTGGACT CCATTTTGGA GGATGAGTAC 
GGTGTGTCTA GGAAGGAGCT GCTTGGAGAG TTGTTTCCAC ACAGTGTGAA TGCTGAGATG 
AGTGGGGATG GCTGGAGAGG AGGATATCCC ATCAGTGGAG GAACACTGGA GAGACCCTCC 
AGACTGAATA CCACAGCGCC ACAAGTATCA CGATCCACCT GTTATGACAT ATCAGCAGAC 
AACCCACAGC TTCTCGCCAA GCTTAGAGAG ATCTCAGAGA GGAAAGAGGA AGATGAGGAG 
CTCAATTATA AGAAGCAGCT GATGGAGAGT CTGCGTAAAA AGCTGACTGT CCTGCGTGAG 
GCACAGCGGG GTCTGCAGGA GGACATTCGG GCCAATGCTC AGCTGGGAGA TGAGGTGGAG 
AGTCTGGTGC TGGCCATCTG CAAGCCCAAC GAGGTGGACA AGTATCGGAT GTTCATCGGC 
GACCTTGACA AGGTGACCAG TCTGCTGCTG TCGCTTTCCG GAAGGCTGAT CCGAGTGGAA 
AGTGCCCTGG ACTGTGTGGA CCCAGAAACT GGACACCAGG AGAGGCTCCA ACTCCTGGAG 
AAGAAGAAGC AGCTGTTGGT TCAGATGGGA GAGGCGCAGG AGCTGAAGGA GCACGTGGAC 
CGGCGCGAGC AGGCCGTCTG CAGAGTGCTG GGATGCTGCC TGACCCCAGA ACAGATGCGG 
GACTACGGTC ACTTTGTGAA GATGAAGGCG GCGCTACTGG TAGAGCAGAG GCAACTGGAT 
GACAAGATAC GGCTCGGCGA GGAGCAGCTC AGGGGACTTC GAGAGAGCCT CGGCCTGGGT 
TTTGGGATGG CGTATGGCCA TTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shroom4 ( XM_009303139.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301414.1 Danio rerio shroom family member 4 (shroom4), transcript variant X2, mRNA.

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CloneID	ODa42960
Clone ID Related Accession (Same CDS sequence)	XM_009303139.2
Accession Version	XM_009303139.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein Shroom4 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303139.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCCAG CCCATAAGAT AGAAGAGGGA GGAAAAGCAG CTCAGTGTAA GAAGTTGAGA 
GTGGGGGATG AACTGGTGAA CATCAACGGC TCTGCTCTCT ACGGCTCACG GCAAGAAGCT 
CTCATTCTCA TCAAAGGTTC CTACAGGGTT CTGAAGATCA TCGTCCGAAG GCGCAGTGTG 
CCAGTGATCC GCCCTCACTC TTGGCACCTG GCAAAGCTGA CAGAGGCACC TTGCACTACG 
GGCACTGGTG ATCCATCCGA TGGCCCTCCG GCTATGCAGC TTCACTTTAC ACCTCTCAAT 
GTACCTTGGC ACTCCGGAGG TGACAACAGT GAGCTGTCCA TGCAGTGGGG CCATATCTCC 
AGGCACTACA GCACTGACCG CAGCAGCTCA ATAGGGAGTA TGGAGAGTCT GGAGAATCCT 
CCTAACCAGG GATACTATGA CAGTCAGCTC TCTCCCATTG ATCCTGTCAT CTTTAACAAC 
AAACGTGACT CTGCTTACAG CTCTTTCTCA GCCAGCTCGA ACACATCTGA TTACACAGTT 
CCTGTCAAAC CTGAGGAGAC CAATTCCATG GACAGTCTTC TTCAGGGTTT GGGTTCTTCC 
TGCAGGTATC CAGACAGAGG CCAACACTCG GCCCCCACTG GCCATGGAAA CCAGCTGGAA 
GAACAAGAAC ACTCCAAGGT GCTGTCTGGC AGATCCGAGC CCAAAGTCCG ACCATCGTCC 
TACAGCTGTG AGGAGGAAAA CTGTGCACCA CCACAGCCAC CAATGAGGAA GGAGAGTTTT 
AGGGCTACTA GAGGCCAAGC AACAGATAAA CGCTGTGTGT CTGCTCCTGT AGGCATCCCA 
AATGTAAGCA GTTGCACGGA TGAAGATCAA TCCCAAATCC AGGATGTTTT GACTGGCAGA 
GTTTATTTGA ACGGAATACA AGACAGTGAG CCAGAGCGTA AAGGGTGCAG CATTCAACCT 
TATTATACCT TGAACTCAGA CTCAGGTGGA GATGGCAAAG CAACTGACCA TGAGAAAGAA 
CACTTGGAGG ACTATTCAAA ATCTGTGGCA GCAGCATCTT CACCTCCTTC ACAAAGGAAT 
AGGGATGAGA ACTTTGATAC CAAACTCCGA CCTGAACATG ATCTCCAGTC AGCAATGAAC 
CGACATAGTG CACCTGAGAA ACTTCTAGCT GCTCAGTTGT GTATGATGGA TGTTTCTGCT 
GACAAAAGTG ATCATAGTGC ATCTCCAACT TGCCAGTGGT CACAGTCCCC TCTTTATCTG 
AGTGATTCCA GTCAAAATAC TGAGCAGGGC AAGTGGGGAA CGAGCAGATG CTCTACACCT 
GGTTCGGTCG CAACTTCGGA AGTCGAAGAT CCCAGACTAG AGGAGGAATC TATTGATGGA 
CAGAATCCAT GGGGGCATCC TATCAGTTTG TCTGGGAGTC CCATTGGAAA TGGATTCTCT 
CAGAACAGTG AGAACAATGG AGGAGAAGTA ATGCTGGATG ATGGCCTTGG CCCAAAGCAG 
CCACAGAAAC GTCAGTTTCG TTGCTCCAAG TCTCGTCGCA GAAGTGAACG TTTTGCCACG 
AACCTCAGAA ATGAGATCCA GAGGAAGAAG GCACAGTTGC AGAAGAGTAG AAACCCTTGC 
GGGGAAGAGA CAGTGGAAGA AGAGGTTGGA GATTTCAACA CTGAGGTTGT AGCTCCTCCA 
GAATACCGTC AACATAAACC CCAAATCTTA CCAAAACCTG CCACCAATAC TGTACCTATA 
CAAATATCTA AACCGGCAAC TCAAAAAGTT TTTGATGCCA CACAAACTGA AGAATATTCC 
AGTGCCAGGG TTGTGTGTTT CCAGACATCT CAGACTCAAA CACAAGTCCC TCAACATGTC 
AGACAAGTGT GTGTACATGT GGTAGAGGAG GTAGCACCTG CAGGCAAACC ACGTCGGTGG 
CGTTGGACGC CAGAACGCAA ACTTGAACTC GAAAATGAAT CATCTGAAAG TAAGAGGAAT 
GAGACTATTG GACAGATGTG GTCTGGAAAA ATGGAATCAA CAAGAAGTAG GACGAGTTCA 
TCCAGCGGTC GTTTAGGTCG ATCGGATGAC TGTGACATCC TTCCCTTTGC AGATCGCAGG 
AGGTTTTTTG AGGAGACTAG CAGAAAGTTG AGCCAGTCTG TAACAAATTT ATCAAACCTG 
ACAAGTCGAA GTCAGAGACT AGACAAGCCG GGCAGAATGA ACCATCCATC ATCACCTGAA 
CCTCATGAGA TTGCTGCTCA CCTTGGCCGT AGGAGATTCT CATATCAAGA TGTGCCCTAC 
ATCAACTCAC TGGACAATGG GAGACAGTTT ATAAGTAACC AACAAGACCA GGAAAAAACG 
AGGCAGAGGC TGATTGAGAG AGAGCAGGAG AGGGAAAGAG AGCAGGAGAG ACTTAAGGAG 
CAAGAGAAGC TTAGAGAACA AGAAAGAGAA AAGGAGAGGT TAAGGAAAGA GAGGGAAAAT 
GAGCAACAAA GGCTGAAGGA CTGGGAGGAA CGGCAGAGGC TGCTACAGAG AGAACAAGAA 
TGGGAGTCTG TGAGAGAATC TGTTCACTCT GAACACAATA CGAGCAGTGA CACAGTTCCT 
CACTCTTTAT CTCATGACGT CTATCGGAAG CAGCCGCCTA GTCCTCAGAT TCCTTCCTCT 
CTGCAAATGT CTGAACCTTT ATATAGCAGC ACTACCACCA ACGTCCAAAA GCCTTGCTCA 
GCGTTCCACC CTGTGACCAC CCAGCACAAC CGGTATGATG GCTACCAAGT AAATCCACCC 
TACAATCCCA GGAACTACAC CCCAGATGAG GCCCAGTATG TTCGGGAACA GGAAAAAACT 
AGAAAGATCA ACAGAAACTT CAGCTTAACA GAGAGGGACT ACCCAAGGTC CAGGAGAGAC 
TTCAAGACAG TAGATGGTGC TAATGTCCCC GGCTTCGTGC GCACAGGAGG CAACAGCATC 
ACAAGTGGAC GCAACGAGGA GCATCTTTTT ACCCCTCTTA GAAACCGTGC CATGTCAGAG 
AATAACCTGT GTGTGGAGAC ACAACATTAT CAAAATCACA GCTCCAGCTC TGGCGTCAGC 
AGAATGCGCT CGTCCACTCT TAGTGACCTG GATGAAAATA CAGTAAGTGT CGGGGAGATC 
AAAAAGAAAA AAGGGCCTCC GCCGCCTCGT CCACCACCAC CAAAATGGGA TCAGTTCCAC 
AAGAGACGGG CGTCCCATCA CAGCCTGTTC TCCTCTACAT CACACTACTC CTCTCCAACT 
GCTTCTCCAC CTCGGCCACA GCCACAGACG TGCACATCAC GACCCCCCAG TGTCTCTGAA 
ATGAGCCGTC AGCGCTCTTA CAGTCTTCCT CCAAGAGACA TGTCCGAGAG TCTTCACTGC 
TGCCGTCAGG AGTACTCCGT CGCTCCCTCC AGTCCTGCTT TTACACGTCG AGCTTTTAAG 
CCTGTAGCCC TGCCGCCACG AGAGATGGAC TATAACACGA GGAGAGAAGT TCACCAGCCT 
GAACCATGCA CCAGAATCCC CATCCATCCT GCTGCAGCAC CAGAAGAGCC CAGAGCCACA 
CATCTGAAGC CCACATTCCC TGATCATGGA GTGGAGTGGG ATCGATCCAG CCCTCACTAC 
CCCACACATG GCACAAGCAG GACTCCTGAA GTGCCCTCAA ACAGTTTCTC AGCTGGGCCT 
TTATGTCCAG AGTCCTACTT CTCCATGAAC AACTTCCAAC TGCAGCCCCA GCAAGCAGGC 
TTCCCCATAA CAGCCCACAA AACCCAGATC ATCCCCTCTC AGGGTCCTGA AGATGGAGAC 
CTGCCTCTGG AGACGGACAT CGATGAGATC TGTGAGAATG AGCAGGCTGA GAGGAAGGAG 
ACGGTGGACC GGATGGAGAT GCAGGGCTTT GCTCGACCTG TGATGGTGTT GGAAACCGAC 
ATTGACCACA CGCCTGAGGA AGCGGCTCCA TCGGCCATAA GCATTAGAGG GCCGAGGGGC 
TCCCTGGTGG ACTCCATTTT GGAGGATGAG TACGGTGTGT CTAGGAAGGA GCTGCTTGGA 
GAGTTGTTTC CACACAGTGT GAATGCTGAG ATGAGTGGGG ATGGCTGGAG AGGAGGATAT 
CCCATCAGTG GAGGAACACT GGAGAGACCC TCCAGACTGA ATACCACAGC GCCACAAGTA 
TCACGATCCA CCTGTTATGA CATATCAGCA GACAACCCAC AGCTTCTCGC CAAGCTTAGA 
GAGATCTCAG AGAGGAAAGA GGAAGATGAG GAGCTCAATT ATAAGAAGCA GCTGATGGAG 
AGTCTGCGTA AAAAGCTGAC TGTCCTGCGT GAGGCACAGC GGGGTCTGCA GGAGGACATT 
CGGGCCAATG CTCAGCTGGG AGATGAGGTG GAGAGTCTGG TGCTGGCCAT CTGCAAGCCC 
AACGAGGTGG ACAAGTATCG GATGTTCATC GGCGACCTTG ACAAGGTGAC CAGTCTGCTG 
CTGTCGCTTT CCGGAAGGCT GATCCGAGTG GAAAGTGCCC TGGACTGTGT GGACCCAGAA 
ACTGGACACC AGGAGAGGCT CCAACTCCTG GAGAAGAAGA AGCAGCTGTT GGTTCAGATG 
GGAGAGGCGC AGGAGCTGAA GGAGCACGTG GACCGGCGCG AGCAGGCCGT CTGCAGAGTG 
CTGGGATGCT GCCTGACCCC AGAACAGATG CGGGACTACG GTCACTTTGT GAAGATGAAG 
GCGGCGCTAC TGGTAGAGCA GAGGCAACTG GATGACAAGA TACGGCTCGG CGAGGAGCAG 
CTCAGGGGAC TTCGAGAGAG CCTCGGCCTG GGTTTTGGGA TGGCGTATGG CCATTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301414.1
CDS	16..4815
Translation	MIPAHKIEEGGKAAQCKKLRVGDELVNINGSALYGSRQEALILIKGSYRVLKIIVRRRSVPVIRPHSWHLAKLTEAPCTTGTGDPSDGPPAMQLHFTPLNVPWHSGGDNSELSMQWGHISRHYSTDRSSSIGSMESLENPPNQGYYDSQLSPIDPVIFNNKRDSAYSSFSASSNTSDYTVPVKPEETNSMDSLLQGLGSSCRYPDRGQHSAPTGHGNQLEEQEHSKVLSGRSEPKVRPSSYSCEEENCAPPQPPMRKESFRATRGQATDKRCVSAPVGIPNVSSCTDEDQSQIQDVLTGRVYLNGIQDSEPERKGCSIQPYYTLNSDSGGDGKATDHEKEHLEDYSKSVAAASSPPSQRNRDENFDTKLRPEHDLQSAMNRHSAPEKLLAAQLCMMDVSADKSDHSASPTCQWSQSPLYLSDSSQNTEQGKWGTSRCSTPGSVATSEVEDPRLEEESIDGQNPWGHPISLSGSPIGNGFSQNSENNGGEVMLDDGLGPKQPQKRQFRCSKSRRRSERFATNLRNEIQRKKAQLQKSRNPCGEETVEEEVGDFNTEVVAPPEYRQHKPQILPKPATNTVPIQISKPATQKVFDATQTEEYSSARVVCFQTSQTQTQVPQHVRQVCVHVVEEVAPAGKPRRWRWTPERKLELENESSESKRNETIGQMWSGKMESTRSRTSSSSGRLGRSDDCDILPFADRRRFFEETSRKLSQSVTNLSNLTSRSQRLDKPGRMNHPSSPEPHEIAAHLGRRRFSYQDVPYINSLDNGRQFISNQQDQEKTRQRLIEREQEREREQERLKEQEKLREQEREKERLRKERENEQQRLKDWEERQRLLQREQEWESVRESVHSEHNTSSDTVPHSLSHDVYRKQPPSPQIPSSLQMSEPLYSSTTTNVQKPCSAFHPVTTQHNRYDGYQVNPPYNPRNYTPDEAQYVREQEKTRKINRNFSLTERDYPRSRRDFKTVDGANVPGFVRTGGNSITSGRNEEHLFTPLRNRAMSENNLCVETQHYQNHSSSSGVSRMRSSTLSDLDENTVSVGEIKKKKGPPPPRPPPPKWDQFHKRRASHHSLFSSTSHYSSPTASPPRPQPQTCTSRPPSVSEMSRQRSYSLPPRDMSESLHCCRQEYSVAPSSPAFTRRAFKPVALPPREMDYNTRREVHQPEPCTRIPIHPAAAPEEPRATHLKPTFPDHGVEWDRSSPHYPTHGTSRTPEVPSNSFSAGPLCPESYFSMNNFQLQPQQAGFPITAHKTQIIPSQGPEDGDLPLETDIDEICENEQAERKETVDRMEMQGFARPVMVLETDIDHTPEEAAPSAISIRGPRGSLVDSILEDEYGVSRKELLGELFPHSVNAEMSGDGWRGGYPISGGTLERPSRLNTTAPQVSRSTCYDISADNPQLLAKLREISERKEEDEELNYKKQLMESLRKKLTVLREAQRGLQEDIRANAQLGDEVESLVLAICKPNEVDKYRMFIGDLDKVTSLLLSLSGRLIRVESALDCVDPETGHQERLQLLEKKKQLLVQMGEAQELKEHVDRREQAVCRVLGCCLTPEQMRDYGHFVKMKAALLVEQRQLDDKIRLGEEQLRGLRESLGLGFGMAYGHY

Target ORF information:

RefSeq Version	XM_009303139.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio shroom family member 4 (shroom4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303139.2

ORF Insert Sequence:

ATGATTCCAG CCCATAAGAT AGAAGAGGGA GGAAAAGCAG CTCAGTGTAA GAAGTTGAGA 
GTGGGGGATG AACTGGTGAA CATCAACGGC TCTGCTCTCT ACGGCTCACG GCAAGAAGCT 
CTCATTCTCA TCAAAGGTTC CTACAGGGTT CTGAAGATCA TCGTCCGAAG GCGCAGTGTG 
CCAGTGATCC GCCCTCACTC TTGGCACCTG GCAAAGCTGA CAGAGGCACC TTGCACTACG 
GGCACTGGTG ATCCATCCGA TGGCCCTCCG GCTATGCAGC TTCACTTTAC ACCTCTCAAT 
GTACCTTGGC ACTCCGGAGG TGACAACAGT GAGCTGTCCA TGCAGTGGGG CCATATCTCC 
AGGCACTACA GCACTGACCG CAGCAGCTCA ATAGGGAGTA TGGAGAGTCT GGAGAATCCT 
CCTAACCAGG GATACTATGA CAGTCAGCTC TCTCCCATTG ATCCTGTCAT CTTTAACAAC 
AAACGTGACT CTGCTTACAG CTCTTTCTCA GCCAGCTCGA ACACATCTGA TTACACAGTT 
CCTGTCAAAC CTGAGGAGAC CAATTCCATG GACAGTCTTC TTCAGGGTTT GGGTTCTTCC 
TGCAGGTATC CAGACAGAGG CCAACACTCG GCCCCCACTG GCCATGGAAA CCAGCTGGAA 
GAACAAGAAC ACTCCAAGGT GCTGTCTGGC AGATCCGAGC CCAAAGTCCG ACCATCGTCC 
TACAGCTGTG AGGAGGAAAA CTGTGCACCA CCACAGCCAC CAATGAGGAA GGAGAGTTTT 
AGGGCTACTA GAGGCCAAGC AACAGATAAA CGCTGTGTGT CTGCTCCTGT AGGCATCCCA 
AATGTAAGCA GTTGCACGGA TGAAGATCAA TCCCAAATCC AGGATGTTTT GACTGGCAGA 
GTTTATTTGA ACGGAATACA AGACAGTGAG CCAGAGCGTA AAGGGTGCAG CATTCAACCT 
TATTATACCT TGAACTCAGA CTCAGGTGGA GATGGCAAAG CAACTGACCA TGAGAAAGAA 
CACTTGGAGG ACTATTCAAA ATCTGTGGCA GCAGCATCTT CACCTCCTTC ACAAAGGAAT 
AGGGATGAGA ACTTTGATAC CAAACTCCGA CCTGAACATG ATCTCCAGTC AGCAATGAAC 
CGACATAGTG CACCTGAGAA ACTTCTAGCT GCTCAGTTGT GTATGATGGA TGTTTCTGCT 
GACAAAAGTG ATCATAGTGC ATCTCCAACT TGCCAGTGGT CACAGTCCCC TCTTTATCTG 
AGTGATTCCA GTCAAAATAC TGAGCAGGGC AAGTGGGGAA CGAGCAGATG CTCTACACCT 
GGTTCGGTCG CAACTTCGGA AGTCGAAGAT CCCAGACTAG AGGAGGAATC TATTGATGGA 
CAGAATCCAT GGGGGCATCC TATCAGTTTG TCTGGGAGTC CCATTGGAAA TGGATTCTCT 
CAGAACAGTG AGAACAATGG AGGAGAAGTA ATGCTGGATG ATGGCCTTGG CCCAAAGCAG 
CCACAGAAAC GTCAGTTTCG TTGCTCCAAG TCTCGTCGCA GAAGTGAACG TTTTGCCACG 
AACCTCAGAA ATGAGATCCA GAGGAAGAAG GCACAGTTGC AGAAGAGTAG AAACCCTTGC 
GGGGAAGAGA CAGTGGAAGA AGAGGTTGGA GATTTCAACA CTGAGGTTGT AGCTCCTCCA 
GAATACCGTC AACATAAACC CCAAATCTTA CCAAAACCTG CCACCAATAC TGTACCTATA 
CAAATATCTA AACCGGCAAC TCAAAAAGTT TTTGATGCCA CACAAACTGA AGAATATTCC 
AGTGCCAGGG TTGTGTGTTT CCAGACATCT CAGACTCAAA CACAAGTCCC TCAACATGTC 
AGACAAGTGT GTGTACATGT GGTAGAGGAG GTAGCACCTG CAGGCAAACC ACGTCGGTGG 
CGTTGGACGC CAGAACGCAA ACTTGAACTC GAAAATGAAT CATCTGAAAG TAAGAGGAAT 
GAGACTATTG GACAGATGTG GTCTGGAAAA ATGGAATCAA CAAGAAGTAG GACGAGTTCA 
TCCAGCGGTC GTTTAGGTCG ATCGGATGAC TGTGACATCC TTCCCTTTGC AGATCGCAGG 
AGGTTTTTTG AGGAGACTAG CAGAAAGTTG AGCCAGTCTG TAACAAATTT ATCAAACCTG 
ACAAGTCGAA GTCAGAGACT AGACAAGCCG GGCAGAATGA ACCATCCATC ATCACCTGAA 
CCTCATGAGA TTGCTGCTCA CCTTGGCCGT AGGAGATTCT CATATCAAGA TGTGCCCTAC 
ATCAACTCAC TGGACAATGG GAGACAGTTT ATAAGTAACC AACAAGACCA GGAAAAAACG 
AGGCAGAGGC TGATTGAGAG AGAGCAGGAG AGGGAAAGAG AGCAGGAGAG ACTTAAGGAG 
CAAGAGAAGC TTAGAGAACA AGAAAGAGAA AAGGAGAGGT TAAGGAAAGA GAGGGAAAAT 
GAGCAACAAA GGCTGAAGGA CTGGGAGGAA CGGCAGAGGC TGCTACAGAG AGAACAAGAA 
TGGGAGTCTG TGAGAGAATC TGTTCACTCT GAACACAATA CGAGCAGTGA CACAGTTCCT 
CACTCTTTAT CTCATGACGT CTATCGGAAG CAGCCGCCTA GTCCTCAGAT TCCTTCCTCT 
CTGCAAATGT CTGAACCTTT ATATAGCAGC ACTACCACCA ACGTCCAAAA GCCTTGCTCA 
GCGTTCCACC CTGTGACCAC CCAGCACAAC CGGTATGATG GCTACCAAGT AAATCCACCC 
TACAATCCCA GGAACTACAC CCCAGATGAG GCCCAGTATG TTCGGGAACA GGAAAAAACT 
AGAAAGATCA ACAGAAACTT CAGCTTAACA GAGAGGGACT ACCCAAGGTC CAGGAGAGAC 
TTCAAGACAG TAGATGGTGC TAATGTCCCC GGCTTCGTGC GCACAGGAGG CAACAGCATC 
ACAAGTGGAC GCAACGAGGA GCATCTTTTT ACCCCTCTTA GAAACCGTGC CATGTCAGAG 
AATAACCTGT GTGTGGAGAC ACAACATTAT CAAAATCACA GCTCCAGCTC TGGCGTCAGC 
AGAATGCGCT CGTCCACTCT TAGTGACCTG GATGAAAATA CAGTAAGTGT CGGGGAGATC 
AAAAAGAAAA AAGGGCCTCC GCCGCCTCGT CCACCACCAC CAAAATGGGA TCAGTTCCAC 
AAGAGACGGG CGTCCCATCA CAGCCTGTTC TCCTCTACAT CACACTACTC CTCTCCAACT 
GCTTCTCCAC CTCGGCCACA GCCACAGACG TGCACATCAC GACCCCCCAG TGTCTCTGAA 
ATGAGCCGTC AGCGCTCTTA CAGTCTTCCT CCAAGAGACA TGTCCGAGAG TCTTCACTGC 
TGCCGTCAGG AGTACTCCGT CGCTCCCTCC AGTCCTGCTT TTACACGTCG AGCTTTTAAG 
CCTGTAGCCC TGCCGCCACG AGAGATGGAC TATAACACGA GGAGAGAAGT TCACCAGCCT 
GAACCATGCA CCAGAATCCC CATCCATCCT GCTGCAGCAC CAGAAGAGCC CAGAGCCACA 
CATCTGAAGC CCACATTCCC TGATCATGGA GTGGAGTGGG ATCGATCCAG CCCTCACTAC 
CCCACACATG GCACAAGCAG GACTCCTGAA GTGCCCTCAA ACAGTTTCTC AGCTGGGCCT 
TTATGTCCAG AGTCCTACTT CTCCATGAAC AACTTCCAAC TGCAGCCCCA GCAAGCAGGC 
TTCCCCATAA CAGCCCACAA AACCCAGATC ATCCCCTCTC AGGGTCCTGA AGATGGAGAC 
CTGCCTCTGG AGACGGACAT CGATGAGATC TGTGAGAATG AGCAGGCTGA GAGGAAGGAG 
ACGGTGGACC GGATGGAGAT GCAGGGCTTT GCTCGACCTG TGATGGTGTT GGAAACCGAC 
ATTGACCACA CGCCTGAGGA AGCGGCTCCA TCGGCCATAA GCATTAGAGG GCCGAGGGGC 
TCCCTGGTGG ACTCCATTTT GGAGGATGAG TACGGTGTGT CTAGGAAGGA GCTGCTTGGA 
GAGTTGTTTC CACACAGTGT GAATGCTGAG ATGAGTGGGG ATGGCTGGAG AGGAGGATAT 
CCCATCAGTG GAGGAACACT GGAGAGACCC TCCAGACTGA ATACCACAGC GCCACAAGTA 
TCACGATCCA CCTGTTATGA CATATCAGCA GACAACCCAC AGCTTCTCGC CAAGCTTAGA 
GAGATCTCAG AGAGGAAAGA GGAAGATGAG GAGCTCAATT ATAAGAAGCA GCTGATGGAG 
AGTCTGCGTA AAAAGCTGAC TGTCCTGCGT GAGGCACAGC GGGGTCTGCA GGAGGACATT 
CGGGCCAATG CTCAGCTGGG AGATGAGGTG GAGAGTCTGG TGCTGGCCAT CTGCAAGCCC 
AACGAGGTGG ACAAGTATCG GATGTTCATC GGCGACCTTG ACAAGGTGAC CAGTCTGCTG 
CTGTCGCTTT CCGGAAGGCT GATCCGAGTG GAAAGTGCCC TGGACTGTGT GGACCCAGAA 
ACTGGACACC AGGAGAGGCT CCAACTCCTG GAGAAGAAGA AGCAGCTGTT GGTTCAGATG 
GGAGAGGCGC AGGAGCTGAA GGAGCACGTG GACCGGCGCG AGCAGGCCGT CTGCAGAGTG 
CTGGGATGCT GCCTGACCCC AGAACAGATG CGGGACTACG GTCACTTTGT GAAGATGAAG 
GCGGCGCTAC TGGTAGAGCA GAGGCAACTG GATGACAAGA TACGGCTCGG CGAGGAGCAG 
CTCAGGGGAC TTCGAGAGAG CCTCGGCCTG GGTTTTGGGA TGGCGTATGG CCATTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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shroom4 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム

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