RUSC1 cDNA ORF clone, Danio rerio(Zebrafish)

The following RUSC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RUSC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa42899 XM_009292152.2
Latest version!
Danio rerio RUN and SH3 domain containing 1 (rusc1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
ODa21246 XM_005157911.4
Latest version!
Danio rerio RUN and SH3 domain containing 1 (rusc1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa21247 XM_005157912.4
Latest version!
Danio rerio RUN and SH3 domain containing 1 (rusc1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa21248 XM_681985.9
Latest version!
Danio rerio RUN and SH3 domain containing 1 (rusc1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa21249 XM_005157913.4
Latest version!
Danio rerio RUN and SH3 domain containing 1 (rusc1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
View Old Accession Versions >>
ODa21248 XM_681985.8 Danio rerio RUN and SH3 domain containing 1 (rusc1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa21246 XM_005157911.3 Danio rerio RUN and SH3 domain containing 1 (rusc1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa21249 XM_005157913.3 Danio rerio RUN and SH3 domain containing 1 (rusc1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa21247 XM_005157912.3 Danio rerio RUN and SH3 domain containing 1 (rusc1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa42899
    Clone ID Related Accession (Same CDS sequence) XM_009292152.2
    Accession Version XM_009292152.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1581bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product RUN and SH3 domain-containing protein 1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292152.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGGAGAGG ATGTGAGGTC CTCAATGAGT GCTGAAGGGC TGTCTTCTGT ATGCCTGCTA 
    GAAAAGAAAA CTCTTGTCAA TTCAGTCAGT GTGGCAGTGG AGGCCATTTT GGCTCAGTTC
    AGTTCATCCA GGACTGTTGT TCAGAAGTTT CACTCAGTAG ATAAGAGCCT TTCTGGAGAC
    AGCAGTGTAA ATCCCTCTCT TGGTCGTCTG GTACTGCAGT GCCTTTGCCC AGCCCTGCGC
    AGCCTGCTCT GTGATGGACT CAAGCCCCAC CAGAGTGACC TCATTGCAGG CAGAAGGCCA
    AATTCACCCT GGGGTTTGGT TCAGGCCTCC ACCAAACCAG GCCCGAGCAC ACAGGCTTTG
    CACAGCCTTC AGACTAAAGT AGCAGGTCTT CATCAGCTAA GGCAGAGCAG GCACAGATTC
    AACGCCTTCC TGTTTGGCCT TCTCAATATC AAGCTTTTGG ATTATTGGCT TTCCCATCTG
    CAGTCTTGCA GCGATGTGCT GGAAACTTTT TATCGGCCTA CTTCCTTCAT GCGTCTGTCA
    CTCACTTCTT GTCAGTCTCT TTTTGAGGAG CTTCTTCTCC TGCTCCAGCC TCTTTCTCTG
    CTGACCTTCA ACCTGGACCT GCTGTTTCAG CATCATCACC TAGACCCTTC TTCTCCAACT
    CTCACCCCAG CCAGCCACAC GTCTGACATA TGCAGCCCAC CAAATGAAGA CTTCAGCTTC
    CACCTGTCAC CCAAGGGCCT TTTCCATGGA GGTGTCCATC GTACTGGAAG CATCTTAGAG
    CAAGACCACA GTTTGAGTTT GGACACCAAC CCTAATTCGT TTTTTTCGAG TCATGTTCTT
    GATGTGTCAG CATATCGCAA TCCAATTTCT CTGTCATCAG GCATCAACAA AGAGGAAGCT
    GATCCGCCAT TATCATGGTT TAACGGGAAT GAGATCTCTG CACCTCTTAA TGCTGGAAGT
    CTTTCCCAGC AAGCGGGCCA GGCTCTTCAG CAAGGCTGGG GGGCAGTCAT GCGCTTGGGG
    GAGCGTTTAG GGCAGAACTT TGGTTTGGCC ACCACTACAG AGAATGCGAA TGGTCCTTAC
    TCACCAGATA ATTCCAAGAC AGGCTACTCA GCAACACCAA GAAGCCCAGT TGAAAACAGG
    CAACAACCTA GAGAGGATCT TTCCCTGTGG GATAGTGGAG CTGCTATTCC TTGGGGTCTT
    GGACGGCTCT TTGGTGCTTC TAAGAGCCCC AACAACCCAC CGACCACCAG GCGCCCATCT
    CAGTGGCTCT CCCCTGGTGT ATCCGTCCTT TCCCGGATAG TGAATCTTAG CCAGGGTTCT
    CCACCTGAGA AAAGAGAGCT TCAGAGAAGC AATGATAAAG AGCAAGATGA GAATGAAAAT
    ATTAATGAAC CAAGAGATCA GCCAAAGCCC CTAAGGGTGG TCAGAACTCT GTGTGATCAC
    ACTGGCACTG GGGCCGAGCT CAGCTTCAGG AAGGGAGAGG AGCTTGTTCT GTTGGGGGGT
    GTCGACCACG ACTGGATTCG ATGTCGCCAA GGTCACCGAG AGGGCCTTGT TCCCATTGGT
    TATGCTTCAC TTATCATGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290427.1
    CDS78..1658
    Translation

    Target ORF information:

    RefSeq Version XM_009292152.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio RUN and SH3 domain containing 1 (rusc1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292152.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGGAGAGG ATGTGAGGTC CTCAATGAGT GCTGAAGGGC TGTCTTCTGT ATGCCTGCTA 
    GAAAAGAAAA CTCTTGTCAA TTCAGTCAGT GTGGCAGTGG AGGCCATTTT GGCTCAGTTC
    AGTTCATCCA GGACTGTTGT TCAGAAGTTT CACTCAGTAG ATAAGAGCCT TTCTGGAGAC
    AGCAGTGTAA ATCCCTCTCT TGGTCGTCTG GTACTGCAGT GCCTTTGCCC AGCCCTGCGC
    AGCCTGCTCT GTGATGGACT CAAGCCCCAC CAGAGTGACC TCATTGCAGG CAGAAGGCCA
    AATTCACCCT GGGGTTTGGT TCAGGCCTCC ACCAAACCAG GCCCGAGCAC ACAGGCTTTG
    CACAGCCTTC AGACTAAAGT AGCAGGTCTT CATCAGCTAA GGCAGAGCAG GCACAGATTC
    AACGCCTTCC TGTTTGGCCT TCTCAATATC AAGCTTTTGG ATTATTGGCT TTCCCATCTG
    CAGTCTTGCA GCGATGTGCT GGAAACTTTT TATCGGCCTA CTTCCTTCAT GCGTCTGTCA
    CTCACTTCTT GTCAGTCTCT TTTTGAGGAG CTTCTTCTCC TGCTCCAGCC TCTTTCTCTG
    CTGACCTTCA ACCTGGACCT GCTGTTTCAG CATCATCACC TAGACCCTTC TTCTCCAACT
    CTCACCCCAG CCAGCCACAC GTCTGACATA TGCAGCCCAC CAAATGAAGA CTTCAGCTTC
    CACCTGTCAC CCAAGGGCCT TTTCCATGGA GGTGTCCATC GTACTGGAAG CATCTTAGAG
    CAAGACCACA GTTTGAGTTT GGACACCAAC CCTAATTCGT TTTTTTCGAG TCATGTTCTT
    GATGTGTCAG CATATCGCAA TCCAATTTCT CTGTCATCAG GCATCAACAA AGAGGAAGCT
    GATCCGCCAT TATCATGGTT TAACGGGAAT GAGATCTCTG CACCTCTTAA TGCTGGAAGT
    CTTTCCCAGC AAGCGGGCCA GGCTCTTCAG CAAGGCTGGG GGGCAGTCAT GCGCTTGGGG
    GAGCGTTTAG GGCAGAACTT TGGTTTGGCC ACCACTACAG AGAATGCGAA TGGTCCTTAC
    TCACCAGATA ATTCCAAGAC AGGCTACTCA GCAACACCAA GAAGCCCAGT TGAAAACAGG
    CAACAACCTA GAGAGGATCT TTCCCTGTGG GATAGTGGAG CTGCTATTCC TTGGGGTCTT
    GGACGGCTCT TTGGTGCTTC TAAGAGCCCC AACAACCCAC CGACCACCAG GCGCCCATCT
    CAGTGGCTCT CCCCTGGTGT ATCCGTCCTT TCCCGGATAG TGAATCTTAG CCAGGGTTCT
    CCACCTGAGA AAAGAGAGCT TCAGAGAAGC AATGATAAAG AGCAAGATGA GAATGAAAAT
    ATTAATGAAC CAAGAGATCA GCCAAAGCCC CTAAGGGTGG TCAGAACTCT GTGTGATCAC
    ACTGGCACTG GGGCCGAGCT CAGCTTCAGG AAGGGAGAGG AGCTTGTTCT GTTGGGGGGT
    GTCGACCACG ACTGGATTCG ATGTCGCCAA GGTCACCGAG AGGGCCTTGT TCCCATTGGT
    TATGCTTCAC TTATCATGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa21246
    Clone ID Related Accession (Same CDS sequence) XM_005157911.3 , XM_005157911.4
    Accession Version XM_005157911.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5652bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product RUN and SH3 domain-containing protein 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005157911.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGCACCCCT CCTCTCGTCC CTCTGTGCCC CCACGTACCC GCAGGTTTGA CAATAGGCGC 
    ACAGAGCCAA AACCAAGTCA AGGGGCCAAG AGGGAGGACA AAAATATGAA CACAGGATCT
    TCATCCCCTC GTCGTGGCAC TCGAGGACCA CCCGAGTCTT CCAGGTTAAA AGGCCCTGGT
    CAAGGTGCAA GGGCCCCAGT GGTTCAGTCC AAACTGACTC GGCCAGTGAG GAATGGTGCT
    CACGCAATAC CAGCAGTCTC AAAACCAAAA AGTGGGCGCG CTGTATCAAA CCTAGCCCCA
    GCTGTGGACC CAAATTGCAA TGAGCCCAGC CTTCCTTGTC TGTGCTGTGA TGGCCATTCA
    CCTCAGGATA GCAACAGCCT TTTTAATCAC AACCATAACA ACAATAACAC TGTAAACATC
    AGGCAACAAA TGAAGATACC GCCACCATCT CCTCCGAATG AGTTGCCTTT TCCAAAACAA
    GAAACTGAAG ACGATAAGTG TGAGCCAGAA GTAAAACAAC CTGATGACTT ACCTTTCCCA
    GTGGAGGAGA AAGAGGAGGA AAATGGTAAT GTGGATAAAA AAGAGGGCGA TGACCTCAAA
    AATGATGACT GCAGTGTAAA CGTGATTGGC AATGTTGATG TGAATGGCAA TGGCAATTGT
    GATGGGGATG ATGATGAAGA TGACGATGAA GATGATGATG ATACTCTTGT TCCTTCTTGC
    TGCAATTGCC CAGAATCCAT GCTGGACTTC TCTCTCACAT CTTCTACCTC ATCTTCTTCC
    ACGTCCATTA GTAGCTGCTC AGATCTGGAG TGTGACTGCC CTGATACATT TTCATTGTCA
    TCCCAGGACC AAGAAGGCCC AGAATGTTAT CCATCTTCTT GTTCCCCACT CTCCAGCTGT
    CCTTCCTTTC AGTCCAATGC CATACCATTG GATCTCAACC TTTCTGCAAG ATCACCTTGC
    TCTTCTGATG AAGGCTATCC ATCAGCACCT TGCTCTCCTT CTGACTACAC AGAGGGCAAG
    TTTTCAGAAG AAGAAAAATG TATTAAAGTG GATCTTCTCC ATTTTTTAGA CTCCATAGAA
    GAGTTGGGTA AAATGGATTT GTTCTACCGC ATTGTTAGGC TGGCACATTG GCAACTTGAT
    GGAGAACTGA TTCTCAGAGA TAGGTTGGAC CACCAGCAGA AGCTTCAAAG GGTCAACAAA
    GAAGTGAAGT TGGCTTATTT TGTGAAACTT CAAGAAGAAG GGTTCAACTT TGGCGATGAG
    GATATCACTG GAGTTTTGGA CGAAATGGGC AACATTGACA TTCCATGGAA GTTATATAAA
    AGCAACAAAT CAAGTGACTC CCAGGAATTT TCTGATGCTG GGGTGGATCT GACAGCTCCT
    TCTGACCTTG ATGAAACTGC TTCCTCTGAT TCACTTGCTC CATCACCATT AGAGCCTCCT
    CCCCGTCCCC CAAAGCCTCC AACAAGGCAT GTGAGTGTTC AGCAGGGATC ACACACTTAT
    GAGAACATCA GTGGGCATAC CTTCTCAGCC CCATCCACCA ATAATGCTAC TTCTTTCTCC
    ACAAGTGTCC CTGTTCCTTC CATGGCATCT ACATCCCCAG CCTTGAAACC TCCTGCCCTT
    CCGCCTCCAC CATCACAGCC TGTGCCCTAC TTCTCTCTTA ATTCAGACAG ACCTGCTCAC
    TCCTCCCCCA CACCACCCAT CCCTCCACCA AGAAGACGTC ATAAAGCCCG ACTAGAGGCT
    CAAAGACTTG CTGAACTTGA AAAAGAAAAG ACTCCTCTAT CCCTTCCACC TCCAACGTCA
    AGACCTCCTC CGTTGCCACC ACCACCTGCT ATGTCCTCTC CCCCAGCTAT CCCACCTCCA
    CCATCAATCC CACCGCCTCC ATCTTTTCAT GCTCTGGATG TGGAAATCCG AAAGTTGCTT
    GCACTAGCAG GACTCACCCA AGCTGAATTG CTTAAACTTA GTCCTGAATT GGGTGTTTGT
    GTAGACGTAG TTGTGGATAA TGAACAGCAT CCAATGTCTG ATGTCTCACA AATTGGAGAA
    ATCAGTGCAA ACAGAGCACA CAAGGAAGAC AGTATGGATA AGCGGTTACA TTGCAGATTG
    AGGGAAGAAA CCAGATTTGG GAAAGATATA CAGAAGGTAG AAACTCTAAG TGAAAACGAG
    ATTTCTAAAG ACGGGCAGAA AGAAGCTTAC AGGACAACAT CCTTCACAGA AATGGCAAGA
    CGACGAAAGA GAAATAGTGG AAACTGTAAC CAAAACTGCA ACTGTGGTTT TGGATCTCCC
    AACTTGTATT ACAGCACTGA TCTTAGCAAT ACAAGCATCC CAAATGTTAG CTTTGGAAGT
    TTTGATTATA CTTCAGTGAT TTCAGACACC CCACCTCCTC CACCTCCACG ACCATTACCC
    CCTTGCCCAC CAGTTATTTC CAATCCTCCT GAACTTCCCC ATCTCAAGCC ATGCACTCTA
    CCTGCCAATG CATCACGACC TGATCGATTT GATTGGCTGA TAGCCTTTAC CCCAGATATT
    GAGCCACCAC CTACTGATAT GCGAAAACCA TCCAATGATG GCATGTTGCC AAAAGGCTCA
    ATTTCAGGCC CAAAAGTCAC CACTTTTAAA GAATTACGCA ATAGAAGCAA ACAGAGCTCC
    CCACCAGCTC CTGTTCAACC AGAACCTGAT CCAACAGTCA TCACTCCAGA CCCTGATTTC
    TTGTACAACC TCAAATGGAG AAGAGAGAAG ACAGATGGAG ATGGCTGGGA ATATACTTCC
    CAAGCACAAG CCTCTTTTCT TCAGCCTCCG CCAACTCCTG CCTCATTGTC CCTCTTTAGG
    GAAATGTGCC GCCTGAATAT ACAAGATGAA TGCAGAGCCG ATCCTAAAGT CTCCCAACAA
    ATGGGCTCAT CAGTAAGTGA AGGCAGCCTT AGGACTATTT GTGGTGATAA AGCTGCTCAC
    ACCAAAAAGA TTCAAAATGA AGATGACCTG GAGGTCAGGG GAATGGCAGA TGGAGCATGG
    ACTTTGGAGT CCAGGACAAC AGTGTCCTCA CCACCCCTGT CCCTTTCCTT GACCTCACCT
    TATTACCTGC ACTCTGCTTC CCTGTCACAA GCCTCCCAAT TTTATCACAC TGACACTACC
    AGTGATCACG CCATGTCCTA TTATGAGGGT TCCGTAAACA TCTCGAATCC CTTATGCATA
    AATTCCACTT TTGACTGCAG CAAACACGAC TATACTGACT CCCTGTACTC CGCCGGCCCG
    GAAAACAATA CATTTTTGTT CTGTAAACAA GAAAATGGTC TCAGTGGTGG CATGGATTCT
    CAGTATCACC ACTGTTATCA TGCTGACAGC TTTCTAGATT CTTTATACGT CTGTGGGCAT
    GAGCCTGGTA GTGCAAAAAA CAGCTTCTGC CCTGTGTATA ACAATGAAGT TGATTCAATC
    AGAAATGACG GTTCACTCTA CAGTGATAGT AGATACTTAG GAAACCCCCT GAAGCTGTTC
    ACTGACCCTG AACCACCGAC TGACATTAAC ACACTCACTG AAGGAGACAT AACACCTTAC
    AAAAACATCG ACATGCAGAC TTACATAACA ATGCGAAACC TTAAAAATAA GAGCAATGCG
    AAAAAATCCA CTTATAAAGA TGCCGACTCG CTTTTTGAGT TGAACTCGGG CAACATTAAT
    GATTCAAAAC CGTCTAACAA TCCATTTTTT GACAGTGGTG AACCCACAAA ACAGGACAAA
    CTTGTATCTT TCCCTGCCTA CTACCTTTAT AACCCTAAAA ACTGTCCCTT GCATAAAGGC
    GCCCCACCAC GGCTTTCCCC CGTAGGTGCG ATCTCTCCTC CCCACAGGTC CGGGCCTCCA
    GTTCCAGGCA CAGATGTTGC CCGTCTCTGT TCACCCCTTT TCCCTCGCAG TCACACCATT
    CCCGCCCTCG CTGCTCCCCT TTATTACCCT TATCTGTACA CCCCCCCTGT TGAAGCTCCT
    GTGCGGGAGC CACCGGTCCC CATACTTCAC TCTCTGCAGA GTCCACCACC GCTCACTTTC
    ACTGTCCGCA GCTTTTCTTT TGCCGGATCT GAGCAGAAGA ATGGGGCCTG GATGGGAGAG
    GATGTGAGGT CCTCAATGAG TGCTGAAGGG CTGTCTTCTG TATGCCTGCT AGAAAAGAAA
    ACTCTTGTCA ATTCAGTCAG TGTGGCAGTG GAGGCCATTT TGGCTCAGTT CAGTTCATCC
    AGGACTGTTG TTCAGAAGTT TCACTCAGTA GATAAGAGCC TTTCTGGAGA CAGCAGTGTA
    AATCCCTCTC TTGGTCGTCT GGTACTGCAG TGCCTTTGCC CAGCCCTGCG CAGCCTGCTC
    TGTGATGGAC TCAAGCCCCA CCAGAGTGAC CTCATTGCAG GCAGAAGGCC AAATTCACCC
    TGGGGTTTGG TTCAGGCCTC CACCAAACCA GGCCCGAGCA CACAGGCTTT GCACAGCCTT
    CAGACTAAAG TAGCAGGTCT TCATCAGCTA AGGCAGAGCA GGCACAGATT CAACGCCTTC
    CTGTTTGGCC TTCTCAATAT CAAGCTTTTG GATTATTGGC TTTCCCATCT GCAGTCTTGC
    AGCGATGTGC TGGAAACTTT TTATCGGCCT ACTTCCTTCA TGCGTCTGTC ACTCACTTCT
    TGTCAGTCTC TTTTTGAGGA GCTTCTTCTC CTGCTCCAGC CTCTTTCTCT GCTGACCTTC
    AACCTGGACC TGCTGTTTCA GCATCATCAC CTAGACCCTT CTTCTCCAAC TCTCACCCCA
    GCCAGCCACA CGTCTGACAT ATGCAGCCCA CCAAATGAAG ACTTCAGCTT CCACCTGTCA
    CCCAAGGGCC TTTTCCATGG AGGTGTCCAT CGTACTGGAA GCATCTTAGA GCAAGACCAC
    AGTTTGAGTT TGGACACCAA CCCTAATTCG TTTTTTTCGA GTCATGTTCT TGATGTGTCA
    GCATATCGCA ATCCAATTTC TCTGTCATCA GGCATCAACA AAGAGGAAGC TGATCCGCCA
    TTATCATGGT TTAACGGGAA TGAGATCTCT GCACCTCTTA ATGCTGGAAG TCTTTCCCAG
    CAAGCGGGCC AGGCTCTTCA GCAAGGCTGG GGGGCAGTCA TGCGCTTGGG GGAGCGTTTA
    GGGCAGAACT TTGGTTTGGC CACCACTACA GAGAATGCGA ATGGTCCTTA CTCACCAGAT
    AATTCCAAGA CAGGCTACTC AGCAACACCA AGAAGCCCAG TTGAAAACAG GCAACAACCT
    AGAGAGGATC TTTCCCTGTG GGATAGTGGA GCTGCTATTC CTTGGGGTCT TGGACGGCTC
    TTTGGTGCTT CTAAGAGCCC CAACAACCCA CCGACCACCA GGCGCCCATC TCAGTGGCTC
    TCCCCTGGTG TATCCGTCCT TTCCCGGATA GTGAATCTTA GCCAGGGTTC TCCACCTGAG
    AAAAGAGAGC TTCAGAGAAG CAATGATAAA GAGCAAGATG AGAATGAAAA TATTAATGAA
    CCAAGAGATC AGCCAAAGCC CCTAAGGGTG GTCAGAACTC TGTGTGATCA CACTGGCACT
    GGGGCCGAGC TCAGCTTCAG GAAGGGAGAG GAGCTTGTTC TGTTGGGGGG TGTCGACCAC
    GACTGGATTC GATGTCGCCA AGGTCACCGA GAGGGCCTTG TTCCCATTGG TTATGCTTCA
    CTTATCATGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005157968.1
    CDS297..5948
    Translation

    Target ORF information:

    RefSeq Version XM_005157911.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio RUN and SH3 domain containing 1 (rusc1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005157911.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGCACCCCT CCTCTCGTCC CTCTGTGCCC CCACGTACCC GCAGGTTTGA CAATAGGCGC 
    ACAGAGCCAA AACCAAGTCA AGGGGCCAAG AGGGAGGACA AAAATATGAA CACAGGATCT
    TCATCCCCTC GTCGTGGCAC TCGAGGACCA CCCGAGTCTT CCAGGTTAAA AGGCCCTGGT
    CAAGGTGCAA GGGCCCCAGT GGTTCAGTCC AAACTGACTC GGCCAGTGAG GAATGGTGCT
    CACGCAATAC CAGCAGTCTC AAAACCAAAA AGTGGGCGCG CTGTATCAAA CCTAGCCCCA
    GCTGTGGACC CAAATTGCAA TGAGCCCAGC CTTCCTTGTC TGTGCTGTGA TGGCCATTCA
    CCTCAGGATA GCAACAGCCT TTTTAATCAC AACCATAACA ACAATAACAC TGTAAACATC
    AGGCAACAAA TGAAGATACC GCCACCATCT CCTCCGAATG AGTTGCCTTT TCCAAAACAA
    GAAACTGAAG ACGATAAGTG TGAGCCAGAA GTAAAACAAC CTGATGACTT ACCTTTCCCA
    GTGGAGGAGA AAGAGGAGGA AAATGGTAAT GTGGATAAAA AAGAGGGCGA TGACCTCAAA
    AATGATGACT GCAGTGTAAA CGTGATTGGC AATGTTGATG TGAATGGCAA TGGCAATTGT
    GATGGGGATG ATGATGAAGA TGACGATGAA GATGATGATG ATACTCTTGT TCCTTCTTGC
    TGCAATTGCC CAGAATCCAT GCTGGACTTC TCTCTCACAT CTTCTACCTC ATCTTCTTCC
    ACGTCCATTA GTAGCTGCTC AGATCTGGAG TGTGACTGCC CTGATACATT TTCATTGTCA
    TCCCAGGACC AAGAAGGCCC AGAATGTTAT CCATCTTCTT GTTCCCCACT CTCCAGCTGT
    CCTTCCTTTC AGTCCAATGC CATACCATTG GATCTCAACC TTTCTGCAAG ATCACCTTGC
    TCTTCTGATG AAGGCTATCC ATCAGCACCT TGCTCTCCTT CTGACTACAC AGAGGGCAAG
    TTTTCAGAAG AAGAAAAATG TATTAAAGTG GATCTTCTCC ATTTTTTAGA CTCCATAGAA
    GAGTTGGGTA AAATGGATTT GTTCTACCGC ATTGTTAGGC TGGCACATTG GCAACTTGAT
    GGAGAACTGA TTCTCAGAGA TAGGTTGGAC CACCAGCAGA AGCTTCAAAG GGTCAACAAA
    GAAGTGAAGT TGGCTTATTT TGTGAAACTT CAAGAAGAAG GGTTCAACTT TGGCGATGAG
    GATATCACTG GAGTTTTGGA CGAAATGGGC AACATTGACA TTCCATGGAA GTTATATAAA
    AGCAACAAAT CAAGTGACTC CCAGGAATTT TCTGATGCTG GGGTGGATCT GACAGCTCCT
    TCTGACCTTG ATGAAACTGC TTCCTCTGAT TCACTTGCTC CATCACCATT AGAGCCTCCT
    CCCCGTCCCC CAAAGCCTCC AACAAGGCAT GTGAGTGTTC AGCAGGGATC ACACACTTAT
    GAGAACATCA GTGGGCATAC CTTCTCAGCC CCATCCACCA ATAATGCTAC TTCTTTCTCC
    ACAAGTGTCC CTGTTCCTTC CATGGCATCT ACATCCCCAG CCTTGAAACC TCCTGCCCTT
    CCGCCTCCAC CATCACAGCC TGTGCCCTAC TTCTCTCTTA ATTCAGACAG ACCTGCTCAC
    TCCTCCCCCA CACCACCCAT CCCTCCACCA AGAAGACGTC ATAAAGCCCG ACTAGAGGCT
    CAAAGACTTG CTGAACTTGA AAAAGAAAAG ACTCCTCTAT CCCTTCCACC TCCAACGTCA
    AGACCTCCTC CGTTGCCACC ACCACCTGCT ATGTCCTCTC CCCCAGCTAT CCCACCTCCA
    CCATCAATCC CACCGCCTCC ATCTTTTCAT GCTCTGGATG TGGAAATCCG AAAGTTGCTT
    GCACTAGCAG GACTCACCCA AGCTGAATTG CTTAAACTTA GTCCTGAATT GGGTGTTTGT
    GTAGACGTAG TTGTGGATAA TGAACAGCAT CCAATGTCTG ATGTCTCACA AATTGGAGAA
    ATCAGTGCAA ACAGAGCACA CAAGGAAGAC AGTATGGATA AGCGGTTACA TTGCAGATTG
    AGGGAAGAAA CCAGATTTGG GAAAGATATA CAGAAGGTAG AAACTCTAAG TGAAAACGAG
    ATTTCTAAAG ACGGGCAGAA AGAAGCTTAC AGGACAACAT CCTTCACAGA AATGGCAAGA
    CGACGAAAGA GAAATAGTGG AAACTGTAAC CAAAACTGCA ACTGTGGTTT TGGATCTCCC
    AACTTGTATT ACAGCACTGA TCTTAGCAAT ACAAGCATCC CAAATGTTAG CTTTGGAAGT
    TTTGATTATA CTTCAGTGAT TTCAGACACC CCACCTCCTC CACCTCCACG ACCATTACCC
    CCTTGCCCAC CAGTTATTTC CAATCCTCCT GAACTTCCCC ATCTCAAGCC ATGCACTCTA
    CCTGCCAATG CATCACGACC TGATCGATTT GATTGGCTGA TAGCCTTTAC CCCAGATATT
    GAGCCACCAC CTACTGATAT GCGAAAACCA TCCAATGATG GCATGTTGCC AAAAGGCTCA
    ATTTCAGGCC CAAAAGTCAC CACTTTTAAA GAATTACGCA ATAGAAGCAA ACAGAGCTCC
    CCACCAGCTC CTGTTCAACC AGAACCTGAT CCAACAGTCA TCACTCCAGA CCCTGATTTC
    TTGTACAACC TCAAATGGAG AAGAGAGAAG ACAGATGGAG ATGGCTGGGA ATATACTTCC
    CAAGCACAAG CCTCTTTTCT TCAGCCTCCG CCAACTCCTG CCTCATTGTC CCTCTTTAGG
    GAAATGTGCC GCCTGAATAT ACAAGATGAA TGCAGAGCCG ATCCTAAAGT CTCCCAACAA
    ATGGGCTCAT CAGTAAGTGA AGGCAGCCTT AGGACTATTT GTGGTGATAA AGCTGCTCAC
    ACCAAAAAGA TTCAAAATGA AGATGACCTG GAGGTCAGGG GAATGGCAGA TGGAGCATGG
    ACTTTGGAGT CCAGGACAAC AGTGTCCTCA CCACCCCTGT CCCTTTCCTT GACCTCACCT
    TATTACCTGC ACTCTGCTTC CCTGTCACAA GCCTCCCAAT TTTATCACAC TGACACTACC
    AGTGATCACG CCATGTCCTA TTATGAGGGT TCCGTAAACA TCTCGAATCC CTTATGCATA
    AATTCCACTT TTGACTGCAG CAAACACGAC TATACTGACT CCCTGTACTC CGCCGGCCCG
    GAAAACAATA CATTTTTGTT CTGTAAACAA GAAAATGGTC TCAGTGGTGG CATGGATTCT
    CAGTATCACC ACTGTTATCA TGCTGACAGC TTTCTAGATT CTTTATACGT CTGTGGGCAT
    GAGCCTGGTA GTGCAAAAAA CAGCTTCTGC CCTGTGTATA ACAATGAAGT TGATTCAATC
    AGAAATGACG GTTCACTCTA CAGTGATAGT AGATACTTAG GAAACCCCCT GAAGCTGTTC
    ACTGACCCTG AACCACCGAC TGACATTAAC ACACTCACTG AAGGAGACAT AACACCTTAC
    AAAAACATCG ACATGCAGAC TTACATAACA ATGCGAAACC TTAAAAATAA GAGCAATGCG
    AAAAAATCCA CTTATAAAGA TGCCGACTCG CTTTTTGAGT TGAACTCGGG CAACATTAAT
    GATTCAAAAC CGTCTAACAA TCCATTTTTT GACAGTGGTG AACCCACAAA ACAGGACAAA
    CTTGTATCTT TCCCTGCCTA CTACCTTTAT AACCCTAAAA ACTGTCCCTT GCATAAAGGC
    GCCCCACCAC GGCTTTCCCC CGTAGGTGCG ATCTCTCCTC CCCACAGGTC CGGGCCTCCA
    GTTCCAGGCA CAGATGTTGC CCGTCTCTGT TCACCCCTTT TCCCTCGCAG TCACACCATT
    CCCGCCCTCG CTGCTCCCCT TTATTACCCT TATCTGTACA CCCCCCCTGT TGAAGCTCCT
    GTGCGGGAGC CACCGGTCCC CATACTTCAC TCTCTGCAGA GTCCACCACC GCTCACTTTC
    ACTGTCCGCA GCTTTTCTTT TGCCGGATCT GAGCAGAAGA ATGGGGCCTG GATGGGAGAG
    GATGTGAGGT CCTCAATGAG TGCTGAAGGG CTGTCTTCTG TATGCCTGCT AGAAAAGAAA
    ACTCTTGTCA ATTCAGTCAG TGTGGCAGTG GAGGCCATTT TGGCTCAGTT CAGTTCATCC
    AGGACTGTTG TTCAGAAGTT TCACTCAGTA GATAAGAGCC TTTCTGGAGA CAGCAGTGTA
    AATCCCTCTC TTGGTCGTCT GGTACTGCAG TGCCTTTGCC CAGCCCTGCG CAGCCTGCTC
    TGTGATGGAC TCAAGCCCCA CCAGAGTGAC CTCATTGCAG GCAGAAGGCC AAATTCACCC
    TGGGGTTTGG TTCAGGCCTC CACCAAACCA GGCCCGAGCA CACAGGCTTT GCACAGCCTT
    CAGACTAAAG TAGCAGGTCT TCATCAGCTA AGGCAGAGCA GGCACAGATT CAACGCCTTC
    CTGTTTGGCC TTCTCAATAT CAAGCTTTTG GATTATTGGC TTTCCCATCT GCAGTCTTGC
    AGCGATGTGC TGGAAACTTT TTATCGGCCT ACTTCCTTCA TGCGTCTGTC ACTCACTTCT
    TGTCAGTCTC TTTTTGAGGA GCTTCTTCTC CTGCTCCAGC CTCTTTCTCT GCTGACCTTC
    AACCTGGACC TGCTGTTTCA GCATCATCAC CTAGACCCTT CTTCTCCAAC TCTCACCCCA
    GCCAGCCACA CGTCTGACAT ATGCAGCCCA CCAAATGAAG ACTTCAGCTT CCACCTGTCA
    CCCAAGGGCC TTTTCCATGG AGGTGTCCAT CGTACTGGAA GCATCTTAGA GCAAGACCAC
    AGTTTGAGTT TGGACACCAA CCCTAATTCG TTTTTTTCGA GTCATGTTCT TGATGTGTCA
    GCATATCGCA ATCCAATTTC TCTGTCATCA GGCATCAACA AAGAGGAAGC TGATCCGCCA
    TTATCATGGT TTAACGGGAA TGAGATCTCT GCACCTCTTA ATGCTGGAAG TCTTTCCCAG
    CAAGCGGGCC AGGCTCTTCA GCAAGGCTGG GGGGCAGTCA TGCGCTTGGG GGAGCGTTTA
    GGGCAGAACT TTGGTTTGGC CACCACTACA GAGAATGCGA ATGGTCCTTA CTCACCAGAT
    AATTCCAAGA CAGGCTACTC AGCAACACCA AGAAGCCCAG TTGAAAACAG GCAACAACCT
    AGAGAGGATC TTTCCCTGTG GGATAGTGGA GCTGCTATTC CTTGGGGTCT TGGACGGCTC
    TTTGGTGCTT CTAAGAGCCC CAACAACCCA CCGACCACCA GGCGCCCATC TCAGTGGCTC
    TCCCCTGGTG TATCCGTCCT TTCCCGGATA GTGAATCTTA GCCAGGGTTC TCCACCTGAG
    AAAAGAGAGC TTCAGAGAAG CAATGATAAA GAGCAAGATG AGAATGAAAA TATTAATGAA
    CCAAGAGATC AGCCAAAGCC CCTAAGGGTG GTCAGAACTC TGTGTGATCA CACTGGCACT
    GGGGCCGAGC TCAGCTTCAG GAAGGGAGAG GAGCTTGTTC TGTTGGGGGG TGTCGACCAC
    GACTGGATTC GATGTCGCCA AGGTCACCGA GAGGGCCTTG TTCCCATTGG TTATGCTTCA
    CTTATCATGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa21246
    Clone ID Related Accession (Same CDS sequence) XM_005157911.3 , XM_005157911.4
    Accession Version XM_005157911.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5652bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product RUN and SH3 domain-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005157911.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGCACCCCT CCTCTCGTCC CTCTGTGCCC CCACGTACCC GCAGGTTTGA CAATAGGCGC 
    ACAGAGCCAA AACCAAGTCA AGGGGCCAAG AGGGAGGACA AAAATATGAA CACAGGATCT
    TCATCCCCTC GTCGTGGCAC TCGAGGACCA CCCGAGTCTT CCAGGTTAAA AGGCCCTGGT
    CAAGGTGCAA GGGCCCCAGT GGTTCAGTCC AAACTGACTC GGCCAGTGAG GAATGGTGCT
    CACGCAATAC CAGCAGTCTC AAAACCAAAA AGTGGGCGCG CTGTATCAAA CCTAGCCCCA
    GCTGTGGACC CAAATTGCAA TGAGCCCAGC CTTCCTTGTC TGTGCTGTGA TGGCCATTCA
    CCTCAGGATA GCAACAGCCT TTTTAATCAC AACCATAACA ACAATAACAC TGTAAACATC
    AGGCAACAAA TGAAGATACC GCCACCATCT CCTCCGAATG AGTTGCCTTT TCCAAAACAA
    GAAACTGAAG ACGATAAGTG TGAGCCAGAA GTAAAACAAC CTGATGACTT ACCTTTCCCA
    GTGGAGGAGA AAGAGGAGGA AAATGGTAAT GTGGATAAAA AAGAGGGCGA TGACCTCAAA
    AATGATGACT GCAGTGTAAA CGTGATTGGC AATGTTGATG TGAATGGCAA TGGCAATTGT
    GATGGGGATG ATGATGAAGA TGACGATGAA GATGATGATG ATACTCTTGT TCCTTCTTGC
    TGCAATTGCC CAGAATCCAT GCTGGACTTC TCTCTCACAT CTTCTACCTC ATCTTCTTCC
    ACGTCCATTA GTAGCTGCTC AGATCTGGAG TGTGACTGCC CTGATACATT TTCATTGTCA
    TCCCAGGACC AAGAAGGCCC AGAATGTTAT CCATCTTCTT GTTCCCCACT CTCCAGCTGT
    CCTTCCTTTC AGTCCAATGC CATACCATTG GATCTCAACC TTTCTGCAAG ATCACCTTGC
    TCTTCTGATG AAGGCTATCC ATCAGCACCT TGCTCTCCTT CTGACTACAC AGAGGGCAAG
    TTTTCAGAAG AAGAAAAATG TATTAAAGTG GATCTTCTCC ATTTTTTAGA CTCCATAGAA
    GAGTTGGGTA AAATGGATTT GTTCTACCGC ATTGTTAGGC TGGCACATTG GCAACTTGAT
    GGAGAACTGA TTCTCAGAGA TAGGTTGGAC CACCAGCAGA AGCTTCAAAG GGTCAACAAA
    GAAGTGAAGT TGGCTTATTT TGTGAAACTT CAAGAAGAAG GGTTCAACTT TGGCGATGAG
    GATATCACTG GAGTTTTGGA CGAAATGGGC AACATTGACA TTCCATGGAA GTTATATAAA
    AGCAACAAAT CAAGTGACTC CCAGGAATTT TCTGATGCTG GGGTGGATCT GACAGCTCCT
    TCTGACCTTG ATGAAACTGC TTCCTCTGAT TCACTTGCTC CATCACCATT AGAGCCTCCT
    CCCCGTCCCC CAAAGCCTCC AACAAGGCAT GTGAGTGTTC AGCAGGGATC ACACACTTAT
    GAGAACATCA GTGGGCATAC CTTCTCAGCC CCATCCACCA ATAATGCTAC TTCTTTCTCC
    ACAAGTGTCC CTGTTCCTTC CATGGCATCT ACATCCCCAG CCTTGAAACC TCCTGCCCTT
    CCGCCTCCAC CATCACAGCC TGTGCCCTAC TTCTCTCTTA ATTCAGACAG ACCTGCTCAC
    TCCTCCCCCA CACCACCCAT CCCTCCACCA AGAAGACGTC ATAAAGCCCG ACTAGAGGCT
    CAAAGACTTG CTGAACTTGA AAAAGAAAAG ACTCCTCTAT CCCTTCCACC TCCAACGTCA
    AGACCTCCTC CGTTGCCACC ACCACCTGCT ATGTCCTCTC CCCCAGCTAT CCCACCTCCA
    CCATCAATCC CACCGCCTCC ATCTTTTCAT GCTCTGGATG TGGAAATCCG AAAGTTGCTT
    GCACTAGCAG GACTCACCCA AGCTGAATTG CTTAAACTTA GTCCTGAATT GGGTGTTTGT
    GTAGACGTAG TTGTGGATAA TGAACAGCAT CCAATGTCTG ATGTCTCACA AATTGGAGAA
    ATCAGTGCAA ACAGAGCACA CAAGGAAGAC AGTATGGATA AGCGGTTACA TTGCAGATTG
    AGGGAAGAAA CCAGATTTGG GAAAGATATA CAGAAGGTAG AAACTCTAAG TGAAAACGAG
    ATTTCTAAAG ACGGGCAGAA AGAAGCTTAC AGGACAACAT CCTTCACAGA AATGGCAAGA
    CGACGAAAGA GAAATAGTGG AAACTGTAAC CAAAACTGCA ACTGTGGTTT TGGATCTCCC
    AACTTGTATT ACAGCACTGA TCTTAGCAAT ACAAGCATCC CAAATGTTAG CTTTGGAAGT
    TTTGATTATA CTTCAGTGAT TTCAGACACC CCACCTCCTC CACCTCCACG ACCATTACCC
    CCTTGCCCAC CAGTTATTTC CAATCCTCCT GAACTTCCCC ATCTCAAGCC ATGCACTCTA
    CCTGCCAATG CATCACGACC TGATCGATTT GATTGGCTGA TAGCCTTTAC CCCAGATATT
    GAGCCACCAC CTACTGATAT GCGAAAACCA TCCAATGATG GCATGTTGCC AAAAGGCTCA
    ATTTCAGGCC CAAAAGTCAC CACTTTTAAA GAATTACGCA ATAGAAGCAA ACAGAGCTCC
    CCACCAGCTC CTGTTCAACC AGAACCTGAT CCAACAGTCA TCACTCCAGA CCCTGATTTC
    TTGTACAACC TCAAATGGAG AAGAGAGAAG ACAGATGGAG ATGGCTGGGA ATATACTTCC
    CAAGCACAAG CCTCTTTTCT TCAGCCTCCG CCAACTCCTG CCTCATTGTC CCTCTTTAGG
    GAAATGTGCC GCCTGAATAT ACAAGATGAA TGCAGAGCCG ATCCTAAAGT CTCCCAACAA
    ATGGGCTCAT CAGTAAGTGA AGGCAGCCTT AGGACTATTT GTGGTGATAA AGCTGCTCAC
    ACCAAAAAGA TTCAAAATGA AGATGACCTG GAGGTCAGGG GAATGGCAGA TGGAGCATGG
    ACTTTGGAGT CCAGGACAAC AGTGTCCTCA CCACCCCTGT CCCTTTCCTT GACCTCACCT
    TATTACCTGC ACTCTGCTTC CCTGTCACAA GCCTCCCAAT TTTATCACAC TGACACTACC
    AGTGATCACG CCATGTCCTA TTATGAGGGT TCCGTAAACA TCTCGAATCC CTTATGCATA
    AATTCCACTT TTGACTGCAG CAAACACGAC TATACTGACT CCCTGTACTC CGCCGGCCCG
    GAAAACAATA CATTTTTGTT CTGTAAACAA GAAAATGGTC TCAGTGGTGG CATGGATTCT
    CAGTATCACC ACTGTTATCA TGCTGACAGC TTTCTAGATT CTTTATACGT CTGTGGGCAT
    GAGCCTGGTA GTGCAAAAAA CAGCTTCTGC CCTGTGTATA ACAATGAAGT TGATTCAATC
    AGAAATGACG GTTCACTCTA CAGTGATAGT AGATACTTAG GAAACCCCCT GAAGCTGTTC
    ACTGACCCTG AACCACCGAC TGACATTAAC ACACTCACTG AAGGAGACAT AACACCTTAC
    AAAAACATCG ACATGCAGAC TTACATAACA ATGCGAAACC TTAAAAATAA GAGCAATGCG
    AAAAAATCCA CTTATAAAGA TGCCGACTCG CTTTTTGAGT TGAACTCGGG CAACATTAAT
    GATTCAAAAC CGTCTAACAA TCCATTTTTT GACAGTGGTG AACCCACAAA ACAGGACAAA
    CTTGTATCTT TCCCTGCCTA CTACCTTTAT AACCCTAAAA ACTGTCCCTT GCATAAAGGC
    GCCCCACCAC GGCTTTCCCC CGTAGGTGCG ATCTCTCCTC CCCACAGGTC CGGGCCTCCA
    GTTCCAGGCA CAGATGTTGC CCGTCTCTGT TCACCCCTTT TCCCTCGCAG TCACACCATT
    CCCGCCCTCG CTGCTCCCCT TTATTACCCT TATCTGTACA CCCCCCCTGT TGAAGCTCCT
    GTGCGGGAGC CACCGGTCCC CATACTTCAC TCTCTGCAGA GTCCACCACC GCTCACTTTC
    ACTGTCCGCA GCTTTTCTTT TGCCGGATCT GAGCAGAAGA ATGGGGCCTG GATGGGAGAG
    GATGTGAGGT CCTCAATGAG TGCTGAAGGG CTGTCTTCTG TATGCCTGCT AGAAAAGAAA
    ACTCTTGTCA ATTCAGTCAG TGTGGCAGTG GAGGCCATTT TGGCTCAGTT CAGTTCATCC
    AGGACTGTTG TTCAGAAGTT TCACTCAGTA GATAAGAGCC TTTCTGGAGA CAGCAGTGTA
    AATCCCTCTC TTGGTCGTCT GGTACTGCAG TGCCTTTGCC CAGCCCTGCG CAGCCTGCTC
    TGTGATGGAC TCAAGCCCCA CCAGAGTGAC CTCATTGCAG GCAGAAGGCC AAATTCACCC
    TGGGGTTTGG TTCAGGCCTC CACCAAACCA GGCCCGAGCA CACAGGCTTT GCACAGCCTT
    CAGACTAAAG TAGCAGGTCT TCATCAGCTA AGGCAGAGCA GGCACAGATT CAACGCCTTC
    CTGTTTGGCC TTCTCAATAT CAAGCTTTTG GATTATTGGC TTTCCCATCT GCAGTCTTGC
    AGCGATGTGC TGGAAACTTT TTATCGGCCT ACTTCCTTCA TGCGTCTGTC ACTCACTTCT
    TGTCAGTCTC TTTTTGAGGA GCTTCTTCTC CTGCTCCAGC CTCTTTCTCT GCTGACCTTC
    AACCTGGACC TGCTGTTTCA GCATCATCAC CTAGACCCTT CTTCTCCAAC TCTCACCCCA
    GCCAGCCACA CGTCTGACAT ATGCAGCCCA CCAAATGAAG ACTTCAGCTT CCACCTGTCA
    CCCAAGGGCC TTTTCCATGG AGGTGTCCAT CGTACTGGAA GCATCTTAGA GCAAGACCAC
    AGTTTGAGTT TGGACACCAA CCCTAATTCG TTTTTTTCGA GTCATGTTCT TGATGTGTCA
    GCATATCGCA ATCCAATTTC TCTGTCATCA GGCATCAACA AAGAGGAAGC TGATCCGCCA
    TTATCATGGT TTAACGGGAA TGAGATCTCT GCACCTCTTA ATGCTGGAAG TCTTTCCCAG
    CAAGCGGGCC AGGCTCTTCA GCAAGGCTGG GGGGCAGTCA TGCGCTTGGG GGAGCGTTTA
    GGGCAGAACT TTGGTTTGGC CACCACTACA GAGAATGCGA ATGGTCCTTA CTCACCAGAT
    AATTCCAAGA CAGGCTACTC AGCAACACCA AGAAGCCCAG TTGAAAACAG GCAACAACCT
    AGAGAGGATC TTTCCCTGTG GGATAGTGGA GCTGCTATTC CTTGGGGTCT TGGACGGCTC
    TTTGGTGCTT CTAAGAGCCC CAACAACCCA CCGACCACCA GGCGCCCATC TCAGTGGCTC
    TCCCCTGGTG TATCCGTCCT TTCCCGGATA GTGAATCTTA GCCAGGGTTC TCCACCTGAG
    AAAAGAGAGC TTCAGAGAAG CAATGATAAA GAGCAAGATG AGAATGAAAA TATTAATGAA
    CCAAGAGATC AGCCAAAGCC CCTAAGGGTG GTCAGAACTC TGTGTGATCA CACTGGCACT
    GGGGCCGAGC TCAGCTTCAG GAAGGGAGAG GAGCTTGTTC TGTTGGGGGG TGTCGACCAC
    GACTGGATTC GATGTCGCCA AGGTCACCGA GAGGGCCTTG TTCCCATTGG TTATGCTTCA
    CTTATCATGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005157968.1
    CDS279..5930
    Translation

    Target ORF information:

    RefSeq Version XM_005157911.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio RUN and SH3 domain containing 1 (rusc1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005157911.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGCACCCCT CCTCTCGTCC CTCTGTGCCC CCACGTACCC GCAGGTTTGA CAATAGGCGC 
    ACAGAGCCAA AACCAAGTCA AGGGGCCAAG AGGGAGGACA AAAATATGAA CACAGGATCT
    TCATCCCCTC GTCGTGGCAC TCGAGGACCA CCCGAGTCTT CCAGGTTAAA AGGCCCTGGT
    CAAGGTGCAA GGGCCCCAGT GGTTCAGTCC AAACTGACTC GGCCAGTGAG GAATGGTGCT
    CACGCAATAC CAGCAGTCTC AAAACCAAAA AGTGGGCGCG CTGTATCAAA CCTAGCCCCA
    GCTGTGGACC CAAATTGCAA TGAGCCCAGC CTTCCTTGTC TGTGCTGTGA TGGCCATTCA
    CCTCAGGATA GCAACAGCCT TTTTAATCAC AACCATAACA ACAATAACAC TGTAAACATC
    AGGCAACAAA TGAAGATACC GCCACCATCT CCTCCGAATG AGTTGCCTTT TCCAAAACAA
    GAAACTGAAG ACGATAAGTG TGAGCCAGAA GTAAAACAAC CTGATGACTT ACCTTTCCCA
    GTGGAGGAGA AAGAGGAGGA AAATGGTAAT GTGGATAAAA AAGAGGGCGA TGACCTCAAA
    AATGATGACT GCAGTGTAAA CGTGATTGGC AATGTTGATG TGAATGGCAA TGGCAATTGT
    GATGGGGATG ATGATGAAGA TGACGATGAA GATGATGATG ATACTCTTGT TCCTTCTTGC
    TGCAATTGCC CAGAATCCAT GCTGGACTTC TCTCTCACAT CTTCTACCTC ATCTTCTTCC
    ACGTCCATTA GTAGCTGCTC AGATCTGGAG TGTGACTGCC CTGATACATT TTCATTGTCA
    TCCCAGGACC AAGAAGGCCC AGAATGTTAT CCATCTTCTT GTTCCCCACT CTCCAGCTGT
    CCTTCCTTTC AGTCCAATGC CATACCATTG GATCTCAACC TTTCTGCAAG ATCACCTTGC
    TCTTCTGATG AAGGCTATCC ATCAGCACCT TGCTCTCCTT CTGACTACAC AGAGGGCAAG
    TTTTCAGAAG AAGAAAAATG TATTAAAGTG GATCTTCTCC ATTTTTTAGA CTCCATAGAA
    GAGTTGGGTA AAATGGATTT GTTCTACCGC ATTGTTAGGC TGGCACATTG GCAACTTGAT
    GGAGAACTGA TTCTCAGAGA TAGGTTGGAC CACCAGCAGA AGCTTCAAAG GGTCAACAAA
    GAAGTGAAGT TGGCTTATTT TGTGAAACTT CAAGAAGAAG GGTTCAACTT TGGCGATGAG
    GATATCACTG GAGTTTTGGA CGAAATGGGC AACATTGACA TTCCATGGAA GTTATATAAA
    AGCAACAAAT CAAGTGACTC CCAGGAATTT TCTGATGCTG GGGTGGATCT GACAGCTCCT
    TCTGACCTTG ATGAAACTGC TTCCTCTGAT TCACTTGCTC CATCACCATT AGAGCCTCCT
    CCCCGTCCCC CAAAGCCTCC AACAAGGCAT GTGAGTGTTC AGCAGGGATC ACACACTTAT
    GAGAACATCA GTGGGCATAC CTTCTCAGCC CCATCCACCA ATAATGCTAC TTCTTTCTCC
    ACAAGTGTCC CTGTTCCTTC CATGGCATCT ACATCCCCAG CCTTGAAACC TCCTGCCCTT
    CCGCCTCCAC CATCACAGCC TGTGCCCTAC TTCTCTCTTA ATTCAGACAG ACCTGCTCAC
    TCCTCCCCCA CACCACCCAT CCCTCCACCA AGAAGACGTC ATAAAGCCCG ACTAGAGGCT
    CAAAGACTTG CTGAACTTGA AAAAGAAAAG ACTCCTCTAT CCCTTCCACC TCCAACGTCA
    AGACCTCCTC CGTTGCCACC ACCACCTGCT ATGTCCTCTC CCCCAGCTAT CCCACCTCCA
    CCATCAATCC CACCGCCTCC ATCTTTTCAT GCTCTGGATG TGGAAATCCG AAAGTTGCTT
    GCACTAGCAG GACTCACCCA AGCTGAATTG CTTAAACTTA GTCCTGAATT GGGTGTTTGT
    GTAGACGTAG TTGTGGATAA TGAACAGCAT CCAATGTCTG ATGTCTCACA AATTGGAGAA
    ATCAGTGCAA ACAGAGCACA CAAGGAAGAC AGTATGGATA AGCGGTTACA TTGCAGATTG
    AGGGAAGAAA CCAGATTTGG GAAAGATATA CAGAAGGTAG AAACTCTAAG TGAAAACGAG
    ATTTCTAAAG ACGGGCAGAA AGAAGCTTAC AGGACAACAT CCTTCACAGA AATGGCAAGA
    CGACGAAAGA GAAATAGTGG AAACTGTAAC CAAAACTGCA ACTGTGGTTT TGGATCTCCC
    AACTTGTATT ACAGCACTGA TCTTAGCAAT ACAAGCATCC CAAATGTTAG CTTTGGAAGT
    TTTGATTATA CTTCAGTGAT TTCAGACACC CCACCTCCTC CACCTCCACG ACCATTACCC
    CCTTGCCCAC CAGTTATTTC CAATCCTCCT GAACTTCCCC ATCTCAAGCC ATGCACTCTA
    CCTGCCAATG CATCACGACC TGATCGATTT GATTGGCTGA TAGCCTTTAC CCCAGATATT
    GAGCCACCAC CTACTGATAT GCGAAAACCA TCCAATGATG GCATGTTGCC AAAAGGCTCA
    ATTTCAGGCC CAAAAGTCAC CACTTTTAAA GAATTACGCA ATAGAAGCAA ACAGAGCTCC
    CCACCAGCTC CTGTTCAACC AGAACCTGAT CCAACAGTCA TCACTCCAGA CCCTGATTTC
    TTGTACAACC TCAAATGGAG AAGAGAGAAG ACAGATGGAG ATGGCTGGGA ATATACTTCC
    CAAGCACAAG CCTCTTTTCT TCAGCCTCCG CCAACTCCTG CCTCATTGTC CCTCTTTAGG
    GAAATGTGCC GCCTGAATAT ACAAGATGAA TGCAGAGCCG ATCCTAAAGT CTCCCAACAA
    ATGGGCTCAT CAGTAAGTGA AGGCAGCCTT AGGACTATTT GTGGTGATAA AGCTGCTCAC
    ACCAAAAAGA TTCAAAATGA AGATGACCTG GAGGTCAGGG GAATGGCAGA TGGAGCATGG
    ACTTTGGAGT CCAGGACAAC AGTGTCCTCA CCACCCCTGT CCCTTTCCTT GACCTCACCT
    TATTACCTGC ACTCTGCTTC CCTGTCACAA GCCTCCCAAT TTTATCACAC TGACACTACC
    AGTGATCACG CCATGTCCTA TTATGAGGGT TCCGTAAACA TCTCGAATCC CTTATGCATA
    AATTCCACTT TTGACTGCAG CAAACACGAC TATACTGACT CCCTGTACTC CGCCGGCCCG
    GAAAACAATA CATTTTTGTT CTGTAAACAA GAAAATGGTC TCAGTGGTGG CATGGATTCT
    CAGTATCACC ACTGTTATCA TGCTGACAGC TTTCTAGATT CTTTATACGT CTGTGGGCAT
    GAGCCTGGTA GTGCAAAAAA CAGCTTCTGC CCTGTGTATA ACAATGAAGT TGATTCAATC
    AGAAATGACG GTTCACTCTA CAGTGATAGT AGATACTTAG GAAACCCCCT GAAGCTGTTC
    ACTGACCCTG AACCACCGAC TGACATTAAC ACACTCACTG AAGGAGACAT AACACCTTAC
    AAAAACATCG ACATGCAGAC TTACATAACA ATGCGAAACC TTAAAAATAA GAGCAATGCG
    AAAAAATCCA CTTATAAAGA TGCCGACTCG CTTTTTGAGT TGAACTCGGG CAACATTAAT
    GATTCAAAAC CGTCTAACAA TCCATTTTTT GACAGTGGTG AACCCACAAA ACAGGACAAA
    CTTGTATCTT TCCCTGCCTA CTACCTTTAT AACCCTAAAA ACTGTCCCTT GCATAAAGGC
    GCCCCACCAC GGCTTTCCCC CGTAGGTGCG ATCTCTCCTC CCCACAGGTC CGGGCCTCCA
    GTTCCAGGCA CAGATGTTGC CCGTCTCTGT TCACCCCTTT TCCCTCGCAG TCACACCATT
    CCCGCCCTCG CTGCTCCCCT TTATTACCCT TATCTGTACA CCCCCCCTGT TGAAGCTCCT
    GTGCGGGAGC CACCGGTCCC CATACTTCAC TCTCTGCAGA GTCCACCACC GCTCACTTTC
    ACTGTCCGCA GCTTTTCTTT TGCCGGATCT GAGCAGAAGA ATGGGGCCTG GATGGGAGAG
    GATGTGAGGT CCTCAATGAG TGCTGAAGGG CTGTCTTCTG TATGCCTGCT AGAAAAGAAA
    ACTCTTGTCA ATTCAGTCAG TGTGGCAGTG GAGGCCATTT TGGCTCAGTT CAGTTCATCC
    AGGACTGTTG TTCAGAAGTT TCACTCAGTA GATAAGAGCC TTTCTGGAGA CAGCAGTGTA
    AATCCCTCTC TTGGTCGTCT GGTACTGCAG TGCCTTTGCC CAGCCCTGCG CAGCCTGCTC
    TGTGATGGAC TCAAGCCCCA CCAGAGTGAC CTCATTGCAG GCAGAAGGCC AAATTCACCC
    TGGGGTTTGG TTCAGGCCTC CACCAAACCA GGCCCGAGCA CACAGGCTTT GCACAGCCTT
    CAGACTAAAG TAGCAGGTCT TCATCAGCTA AGGCAGAGCA GGCACAGATT CAACGCCTTC
    CTGTTTGGCC TTCTCAATAT CAAGCTTTTG GATTATTGGC TTTCCCATCT GCAGTCTTGC
    AGCGATGTGC TGGAAACTTT TTATCGGCCT ACTTCCTTCA TGCGTCTGTC ACTCACTTCT
    TGTCAGTCTC TTTTTGAGGA GCTTCTTCTC CTGCTCCAGC CTCTTTCTCT GCTGACCTTC
    AACCTGGACC TGCTGTTTCA GCATCATCAC CTAGACCCTT CTTCTCCAAC TCTCACCCCA
    GCCAGCCACA CGTCTGACAT ATGCAGCCCA CCAAATGAAG ACTTCAGCTT CCACCTGTCA
    CCCAAGGGCC TTTTCCATGG AGGTGTCCAT CGTACTGGAA GCATCTTAGA GCAAGACCAC
    AGTTTGAGTT TGGACACCAA CCCTAATTCG TTTTTTTCGA GTCATGTTCT TGATGTGTCA
    GCATATCGCA ATCCAATTTC TCTGTCATCA GGCATCAACA AAGAGGAAGC TGATCCGCCA
    TTATCATGGT TTAACGGGAA TGAGATCTCT GCACCTCTTA ATGCTGGAAG TCTTTCCCAG
    CAAGCGGGCC AGGCTCTTCA GCAAGGCTGG GGGGCAGTCA TGCGCTTGGG GGAGCGTTTA
    GGGCAGAACT TTGGTTTGGC CACCACTACA GAGAATGCGA ATGGTCCTTA CTCACCAGAT
    AATTCCAAGA CAGGCTACTC AGCAACACCA AGAAGCCCAG TTGAAAACAG GCAACAACCT
    AGAGAGGATC TTTCCCTGTG GGATAGTGGA GCTGCTATTC CTTGGGGTCT TGGACGGCTC
    TTTGGTGCTT CTAAGAGCCC CAACAACCCA CCGACCACCA GGCGCCCATC TCAGTGGCTC
    TCCCCTGGTG TATCCGTCCT TTCCCGGATA GTGAATCTTA GCCAGGGTTC TCCACCTGAG
    AAAAGAGAGC TTCAGAGAAG CAATGATAAA GAGCAAGATG AGAATGAAAA TATTAATGAA
    CCAAGAGATC AGCCAAAGCC CCTAAGGGTG GTCAGAACTC TGTGTGATCA CACTGGCACT
    GGGGCCGAGC TCAGCTTCAG GAAGGGAGAG GAGCTTGTTC TGTTGGGGGG TGTCGACCAC
    GACTGGATTC GATGTCGCCA AGGTCACCGA GAGGGCCTTG TTCCCATTGG TTATGCTTCA
    CTTATCATGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa21247
    Clone ID Related Accession (Same CDS sequence) XM_005157912.4 , XM_005157912.3
    Accession Version XM_005157912.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5634bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product RUN and SH3 domain-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005157912.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGCACCCCT CCTCTCGTCC CTCTGTGCCC CCACGTACCC GCAGGTTTGA CAATAGGCGC 
    ACAGAGCCAA AACCAAGTCA AGGGGCCAAG AGGGAGGACA AAAATATGAA CACAGGATCT
    TCATCCCCTC GTCGTGGCAC TCGAGGACCA CCCGAGTCTT CCAGGTTAAA AGGCCCTGGT
    CAAGGTGCAA GGGCCCCAGT GGTTCAGTCC AAACTGACTC GGCCAGTGAG GAATGGTGCT
    CACGCAATAC CAGCAGTCTC AAAACCAAAA AGTGGGCGCG CTGTATCAAA CCTAGCCCCA
    GCTGTGGACC CAAATTGCAA TGAGCCCAGC CTTCCTTGTC TGTGCTGTGA TGGCCATTCA
    CCTCAGGATA GCAACAGCCT TTTTAATCAC AACCATAACA ACAATAACAC TGTAAACATC
    AGGCAACAAA TGAAGATACC GCCACCATCT CCTCCGAATG AGTTGCCTTT TCCAAAACAA
    GAAACTGAAG ACGATAAGTG TGAGCCAGAA GTAAAACAAC CTGATGACTT ACCTTTCCCA
    GTGGAGGAGA AAGAGGAGGA AAATGGTAAT GTGGATAAAA AAGAGGGCGA TGACCTCAAA
    AATGATGACT GCAGTGTAAA CGTGATTGGC AATGTTGATG TGAATGGCAA TGGCAATTGT
    GATGGGGATG ATGATGAAGA TGACGATGAA GATGATGATG ATACTCTTGT TCCTTCTTGC
    TGCAATTGCC CAGAATCCAT GCTGGACTTC TCTCTCACAT CTTCTACCTC ATCTTCTTCC
    ACGTCCATTA GTAGCTGCTC AGATCTGGAG TGTGACTGCC CTGATACATT TTCATTGTCA
    TCCCAGGACC AAGAAGGCCC AGAATGTTAT CCATCTTCTT GTTCCCCACT CTCCAGCTGT
    CCTTCCTTTC AGTCCAATGC CATACCATTG GATCTCAACC TTTCTGCAAG ATCACCTTGC
    TCTTCTGATG AAGGCTATCC ATCAGCACCT TGCTCTCCTT CTGACTACAC AGAGGGCAAG
    TTTTCAGAAG AAGAAAAATG TATTAAAGTG GATCTTCTCC ATTTTTTAGA CTCCATAGAA
    GAGTTGGGTA AAATGGATTT GTTCTACCGC ATTGTTAGGC TGGCACATTG GCAACTTGAT
    GGAGAACTGA TTCTCAGAGA TAGGTTGGAC CACCAGCAGA AGCTTCAAAG GGTCAACAAA
    GAAGTGAAGT TGGCTTATTT TGTGAAACTT CAAGAAGAAG GGTTCAACTT TGGCGATGAG
    GATATCACTG GAGTTTTGGA CGAAATGGGC AACATTGACA TTCCATGGAA GTTATATAAA
    AGCAACAAAT CAAGTGACTC CCAGGAATTT TCTGATGCTG GGGTGGATCT GACAGCTCCT
    TCTGACCTTG ATGAAACTGC TTCCTCTGAT TCACTTGCTC CATCACCATT AGAGCCTCCT
    CCCCGTCCCC CAAAGCCTCC AACAAGGCAT GTGAGTGTTC AGCAGGGATC ACACACTTAT
    GAGAACATCA GTGGGCATAC CTTCTCAGCC CCATCCACCA ATAATGCTAC TTCTTTCTCC
    ACAAGTGTCC CTGTTCCTTC CATGGCATCT ACATCCCCAG CCTTGAAACC TCCTGCCCTT
    CCGCCTCCAC CATCACAGCC TGTGCCCTAC TTCTCTCTTA ATTCAGACAG ACCTGCTCAC
    TCCTCCCCCA CACCACCCAT CCCTCCACCA AGAAGACGTC ATAAAGCCCG ACTAGAGGCT
    CAAAGACTTG CTGAACTTGA AAAAGAAAAG ACTCCTCTAT CCCTTCCACC TCCAACGTCA
    AGACCTCCTC CGTTGCCACC ACCACCTGCT ATGTCCTCTC CCCCAGCTAT CCCACCTCCA
    CCATCAATCC CACCGCCTCC ATCTTTTCAT GCTCTGGATG TGGAAATCCG AAAGTTGCTT
    GCACTAGCAG GACTCACCCA AGCTGAATTG CTTAAACTTA GTCCTGAATT GGGTGTTTGT
    GTAGACGTAG TTGTGGATAA TGAACAGCAT CCAATGTCTG ATGTCTCACA AATTGGAGAA
    ATCAGTGCAA ACAGAGCACA CAAGGAAGAC AGTATGGATA AGCGGTTACA TTGCAGATTG
    AGGGAAGAAA CCAGATTTGG GAAAGATATA CAGAAGGTAG AAACTCTAAG TGAAAACGAG
    ATTTCTAAAG ACGGGCAGAA AGAAGCTTAC AGGACAACAT CCTTCACAGA AATGGCAAGA
    CGACGAAAGA GAAATAGTGG AAACTGTAAC CAAAACTGCA ACTGTGGTTT TGGATCTCCC
    AACTTGTATT ACAGCACTGA TCTTAGCAAT ACAAGCATCC CAAATGTTAG CTTTGGAAGT
    TTTGATTATA CTTCAGTGAT TTCAGACACC CCACCTCCTC CACCTCCACG ACCATTACCC
    CCTTGCCCAC CAGTTATTTC CAATCCTCCT GAACTTCCCC ATCTCAAGCC ATGCACTCTA
    CCTGCCAATG CATCACGACC TGATCGATTT GATTGGCTGA TAGCCTTTAC CCCAGATATT
    GAGCCACCAC CTACTGATAT GCGAAAACCA TCCAATGATG GCATGTTGCC AAAAGGCTCA
    ATTTCAGGCC CAAAAGTCAC CACTTTTAAA GAATTACGCA ATAGAAGCAA ACAGAGCTCC
    CCACCAGCTC CTGTTCAACC AGAACCTGAT CCAACAGTCA TCACTCCAGA CCCTGATTTC
    TTGTACAACC TCAAATGGAG AAGAGAGAAG ACAGATGGAG ATGGCTGGGA ATATACTTCC
    CAAGCACAAG CCTCTTTTCT TCAGCCTCCG CCAACTCCTG CCTCATTGTC CCTCTTTAGG
    GAAATGTGCC GCCTGAATAT ACAAGATGAA TGCAGAGCCG ATCCTAAAGT CTCCCAACAA
    ATGGGCTCAT CAGTAAGTGA AGGCAGCCTT AGGACTATTT GTGGTGATAA AGCTGCTCAC
    ACCAAAAAGA TTCAAAATGA AGATGACCTG GAGGTCAGGG GAATGGCAGA TGGAGCATGG
    ACTTTGGAGT CCAGGACAAC AGTGTCCTCA CCACCCCTGT CCCTTTCCTT GACCTCACCT
    TATTACCTGC ACTCTGCTTC CCTGTCACAA GCCTCCCAAT TTTATCACAC TGACACTACC
    AGTGATCACG CCATGTCCTA TTATGAGGGT TCCGTAAACA TCTCGAATCC CTTATGCATA
    AATTCCACTT TTGACTGCAG CAAACACGAC TATACTGACT CCCTGTACTC CGCCGGCCCG
    GAAAACAATA CATTTTTGTT CTGTAAACAA GAAAATGGTC TCAGTGGTGG CATGGATTCT
    CAGTATCACC ACTGTTATCA TGCTGACAGC TTTCTAGATT CTTTATACGT CTGTGGGCAT
    GAGCCTGGTA GTGCAAAAAA CAGCTTCTGC CCTGTGTATA ACAATGAAGT TGATTCAATC
    AGAAATGACG GTTCACTCTA CAGTGATAGT AGATACTTAG GAAACCCCCT GAAGCTGTTC
    ACTGACCCTG AACCACCGAC TGACATTAAC ACACTCACTG AAGGAGACAT AACACCTTAC
    AAAAACATCG ACATGCAGAC TTACATAACA ATGCGAAACC TTAAAAATAA GAGCAATGCG
    AAAAAATCCA CTTATAAAGA TGCCGACTCG CTTTTTGAGT TGAACTCGGG CAACATTAAT
    GATTCAAAAC CGTCTAACAA TCCATTTTTT GACAGTGGTG AACCCACAAA ACAGGACAAA
    CTTGTATCTT TCCCTGCCTA CTACCTTTAT AACCCTAAAA ACTGTCCCTT GCATAAAGGC
    GCCCCACCAC GGCTTTCCCC CGTAGGTGCG ATCTCTCCTC CCCACAGGTC CGGGCCTCCA
    GTTCCAGGCA CAGATGTTGC CCGTCTCTGT TCACCCCTTT TCCCTCGCAG TCACACCATT
    CCCGCCCTCG CTGCTCCCCT TTATTACCCT TATCTGTACA CCCCCCCTGT TGAAGCTCCT
    GTGCGGGAGC CACCGGTCCC CATACTTCAC TCTCTGCAGA GTCCACCACC GCTCACTTTC
    ACTGTCCGCA GCTTTTCTTT TGCCGGATCT GAGCAGAAGA ATGGGGCCTG GATGGGAGAG
    GATGTGAGGT CCTCAATGAG TGCTGAAGGG CTGTCTTCTG TATGCCTGCT AGAAAAGAAA
    ACTCTTGTCA ATTCAGTCAG TGTGGCAGTG GAGGCCATTT TGGCTCAGTT CAGTTCATCC
    AGGACTGTTG TTCAGAAGAG CCTTTCTGGA GACAGCAGTG TAAATCCCTC TCTTGGTCGT
    CTGGTACTGC AGTGCCTTTG CCCAGCCCTG CGCAGCCTGC TCTGTGATGG ACTCAAGCCC
    CACCAGAGTG ACCTCATTGC AGGCAGAAGG CCAAATTCAC CCTGGGGTTT GGTTCAGGCC
    TCCACCAAAC CAGGCCCGAG CACACAGGCT TTGCACAGCC TTCAGACTAA AGTAGCAGGT
    CTTCATCAGC TAAGGCAGAG CAGGCACAGA TTCAACGCCT TCCTGTTTGG CCTTCTCAAT
    ATCAAGCTTT TGGATTATTG GCTTTCCCAT CTGCAGTCTT GCAGCGATGT GCTGGAAACT
    TTTTATCGGC CTACTTCCTT CATGCGTCTG TCACTCACTT CTTGTCAGTC TCTTTTTGAG
    GAGCTTCTTC TCCTGCTCCA GCCTCTTTCT CTGCTGACCT TCAACCTGGA CCTGCTGTTT
    CAGCATCATC ACCTAGACCC TTCTTCTCCA ACTCTCACCC CAGCCAGCCA CACGTCTGAC
    ATATGCAGCC CACCAAATGA AGACTTCAGC TTCCACCTGT CACCCAAGGG CCTTTTCCAT
    GGAGGTGTCC ATCGTACTGG AAGCATCTTA GAGCAAGACC ACAGTTTGAG TTTGGACACC
    AACCCTAATT CGTTTTTTTC GAGTCATGTT CTTGATGTGT CAGCATATCG CAATCCAATT
    TCTCTGTCAT CAGGCATCAA CAAAGAGGAA GCTGATCCGC CATTATCATG GTTTAACGGG
    AATGAGATCT CTGCACCTCT TAATGCTGGA AGTCTTTCCC AGCAAGCGGG CCAGGCTCTT
    CAGCAAGGCT GGGGGGCAGT CATGCGCTTG GGGGAGCGTT TAGGGCAGAA CTTTGGTTTG
    GCCACCACTA CAGAGAATGC GAATGGTCCT TACTCACCAG ATAATTCCAA GACAGGCTAC
    TCAGCAACAC CAAGAAGCCC AGTTGAAAAC AGGCAACAAC CTAGAGAGGA TCTTTCCCTG
    TGGGATAGTG GAGCTGCTAT TCCTTGGGGT CTTGGACGGC TCTTTGGTGC TTCTAAGAGC
    CCCAACAACC CACCGACCAC CAGGCGCCCA TCTCAGTGGC TCTCCCCTGG TGTATCCGTC
    CTTTCCCGGA TAGTGAATCT TAGCCAGGGT TCTCCACCTG AGAAAAGAGA GCTTCAGAGA
    AGCAATGATA AAGAGCAAGA TGAGAATGAA AATATTAATG AACCAAGAGA TCAGCCAAAG
    CCCCTAAGGG TGGTCAGAAC TCTGTGTGAT CACACTGGCA CTGGGGCCGA GCTCAGCTTC
    AGGAAGGGAG AGGAGCTTGT TCTGTTGGGG GGTGTCGACC ACGACTGGAT TCGATGTCGC
    CAAGGTCACC GAGAGGGCCT TGTTCCCATT GGTTATGCTT CACTTATCAT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005157969.1
    CDS279..5912
    Translation

    Target ORF information:

    RefSeq Version XM_005157912.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio RUN and SH3 domain containing 1 (rusc1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005157912.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGCACCCCT CCTCTCGTCC CTCTGTGCCC CCACGTACCC GCAGGTTTGA CAATAGGCGC 
    ACAGAGCCAA AACCAAGTCA AGGGGCCAAG AGGGAGGACA AAAATATGAA CACAGGATCT
    TCATCCCCTC GTCGTGGCAC TCGAGGACCA CCCGAGTCTT CCAGGTTAAA AGGCCCTGGT
    CAAGGTGCAA GGGCCCCAGT GGTTCAGTCC AAACTGACTC GGCCAGTGAG GAATGGTGCT
    CACGCAATAC CAGCAGTCTC AAAACCAAAA AGTGGGCGCG CTGTATCAAA CCTAGCCCCA
    GCTGTGGACC CAAATTGCAA TGAGCCCAGC CTTCCTTGTC TGTGCTGTGA TGGCCATTCA
    CCTCAGGATA GCAACAGCCT TTTTAATCAC AACCATAACA ACAATAACAC TGTAAACATC
    AGGCAACAAA TGAAGATACC GCCACCATCT CCTCCGAATG AGTTGCCTTT TCCAAAACAA
    GAAACTGAAG ACGATAAGTG TGAGCCAGAA GTAAAACAAC CTGATGACTT ACCTTTCCCA
    GTGGAGGAGA AAGAGGAGGA AAATGGTAAT GTGGATAAAA AAGAGGGCGA TGACCTCAAA
    AATGATGACT GCAGTGTAAA CGTGATTGGC AATGTTGATG TGAATGGCAA TGGCAATTGT
    GATGGGGATG ATGATGAAGA TGACGATGAA GATGATGATG ATACTCTTGT TCCTTCTTGC
    TGCAATTGCC CAGAATCCAT GCTGGACTTC TCTCTCACAT CTTCTACCTC ATCTTCTTCC
    ACGTCCATTA GTAGCTGCTC AGATCTGGAG TGTGACTGCC CTGATACATT TTCATTGTCA
    TCCCAGGACC AAGAAGGCCC AGAATGTTAT CCATCTTCTT GTTCCCCACT CTCCAGCTGT
    CCTTCCTTTC AGTCCAATGC CATACCATTG GATCTCAACC TTTCTGCAAG ATCACCTTGC
    TCTTCTGATG AAGGCTATCC ATCAGCACCT TGCTCTCCTT CTGACTACAC AGAGGGCAAG
    TTTTCAGAAG AAGAAAAATG TATTAAAGTG GATCTTCTCC ATTTTTTAGA CTCCATAGAA
    GAGTTGGGTA AAATGGATTT GTTCTACCGC ATTGTTAGGC TGGCACATTG GCAACTTGAT
    GGAGAACTGA TTCTCAGAGA TAGGTTGGAC CACCAGCAGA AGCTTCAAAG GGTCAACAAA
    GAAGTGAAGT TGGCTTATTT TGTGAAACTT CAAGAAGAAG GGTTCAACTT TGGCGATGAG
    GATATCACTG GAGTTTTGGA CGAAATGGGC AACATTGACA TTCCATGGAA GTTATATAAA
    AGCAACAAAT CAAGTGACTC CCAGGAATTT TCTGATGCTG GGGTGGATCT GACAGCTCCT
    TCTGACCTTG ATGAAACTGC TTCCTCTGAT TCACTTGCTC CATCACCATT AGAGCCTCCT
    CCCCGTCCCC CAAAGCCTCC AACAAGGCAT GTGAGTGTTC AGCAGGGATC ACACACTTAT
    GAGAACATCA GTGGGCATAC CTTCTCAGCC CCATCCACCA ATAATGCTAC TTCTTTCTCC
    ACAAGTGTCC CTGTTCCTTC CATGGCATCT ACATCCCCAG CCTTGAAACC TCCTGCCCTT
    CCGCCTCCAC CATCACAGCC TGTGCCCTAC TTCTCTCTTA ATTCAGACAG ACCTGCTCAC
    TCCTCCCCCA CACCACCCAT CCCTCCACCA AGAAGACGTC ATAAAGCCCG ACTAGAGGCT
    CAAAGACTTG CTGAACTTGA AAAAGAAAAG ACTCCTCTAT CCCTTCCACC TCCAACGTCA
    AGACCTCCTC CGTTGCCACC ACCACCTGCT ATGTCCTCTC CCCCAGCTAT CCCACCTCCA
    CCATCAATCC CACCGCCTCC ATCTTTTCAT GCTCTGGATG TGGAAATCCG AAAGTTGCTT
    GCACTAGCAG GACTCACCCA AGCTGAATTG CTTAAACTTA GTCCTGAATT GGGTGTTTGT
    GTAGACGTAG TTGTGGATAA TGAACAGCAT CCAATGTCTG ATGTCTCACA AATTGGAGAA
    ATCAGTGCAA ACAGAGCACA CAAGGAAGAC AGTATGGATA AGCGGTTACA TTGCAGATTG
    AGGGAAGAAA CCAGATTTGG GAAAGATATA CAGAAGGTAG AAACTCTAAG TGAAAACGAG
    ATTTCTAAAG ACGGGCAGAA AGAAGCTTAC AGGACAACAT CCTTCACAGA AATGGCAAGA
    CGACGAAAGA GAAATAGTGG AAACTGTAAC CAAAACTGCA ACTGTGGTTT TGGATCTCCC
    AACTTGTATT ACAGCACTGA TCTTAGCAAT ACAAGCATCC CAAATGTTAG CTTTGGAAGT
    TTTGATTATA CTTCAGTGAT TTCAGACACC CCACCTCCTC CACCTCCACG ACCATTACCC
    CCTTGCCCAC CAGTTATTTC CAATCCTCCT GAACTTCCCC ATCTCAAGCC ATGCACTCTA
    CCTGCCAATG CATCACGACC TGATCGATTT GATTGGCTGA TAGCCTTTAC CCCAGATATT
    GAGCCACCAC CTACTGATAT GCGAAAACCA TCCAATGATG GCATGTTGCC AAAAGGCTCA
    ATTTCAGGCC CAAAAGTCAC CACTTTTAAA GAATTACGCA ATAGAAGCAA ACAGAGCTCC
    CCACCAGCTC CTGTTCAACC AGAACCTGAT CCAACAGTCA TCACTCCAGA CCCTGATTTC
    TTGTACAACC TCAAATGGAG AAGAGAGAAG ACAGATGGAG ATGGCTGGGA ATATACTTCC
    CAAGCACAAG CCTCTTTTCT TCAGCCTCCG CCAACTCCTG CCTCATTGTC CCTCTTTAGG
    GAAATGTGCC GCCTGAATAT ACAAGATGAA TGCAGAGCCG ATCCTAAAGT CTCCCAACAA
    ATGGGCTCAT CAGTAAGTGA AGGCAGCCTT AGGACTATTT GTGGTGATAA AGCTGCTCAC
    ACCAAAAAGA TTCAAAATGA AGATGACCTG GAGGTCAGGG GAATGGCAGA TGGAGCATGG
    ACTTTGGAGT CCAGGACAAC AGTGTCCTCA CCACCCCTGT CCCTTTCCTT GACCTCACCT
    TATTACCTGC ACTCTGCTTC CCTGTCACAA GCCTCCCAAT TTTATCACAC TGACACTACC
    AGTGATCACG CCATGTCCTA TTATGAGGGT TCCGTAAACA TCTCGAATCC CTTATGCATA
    AATTCCACTT TTGACTGCAG CAAACACGAC TATACTGACT CCCTGTACTC CGCCGGCCCG
    GAAAACAATA CATTTTTGTT CTGTAAACAA GAAAATGGTC TCAGTGGTGG CATGGATTCT
    CAGTATCACC ACTGTTATCA TGCTGACAGC TTTCTAGATT CTTTATACGT CTGTGGGCAT
    GAGCCTGGTA GTGCAAAAAA CAGCTTCTGC CCTGTGTATA ACAATGAAGT TGATTCAATC
    AGAAATGACG GTTCACTCTA CAGTGATAGT AGATACTTAG GAAACCCCCT GAAGCTGTTC
    ACTGACCCTG AACCACCGAC TGACATTAAC ACACTCACTG AAGGAGACAT AACACCTTAC
    AAAAACATCG ACATGCAGAC TTACATAACA ATGCGAAACC TTAAAAATAA GAGCAATGCG
    AAAAAATCCA CTTATAAAGA TGCCGACTCG CTTTTTGAGT TGAACTCGGG CAACATTAAT
    GATTCAAAAC CGTCTAACAA TCCATTTTTT GACAGTGGTG AACCCACAAA ACAGGACAAA
    CTTGTATCTT TCCCTGCCTA CTACCTTTAT AACCCTAAAA ACTGTCCCTT GCATAAAGGC
    GCCCCACCAC GGCTTTCCCC CGTAGGTGCG ATCTCTCCTC CCCACAGGTC CGGGCCTCCA
    GTTCCAGGCA CAGATGTTGC CCGTCTCTGT TCACCCCTTT TCCCTCGCAG TCACACCATT
    CCCGCCCTCG CTGCTCCCCT TTATTACCCT TATCTGTACA CCCCCCCTGT TGAAGCTCCT
    GTGCGGGAGC CACCGGTCCC CATACTTCAC TCTCTGCAGA GTCCACCACC GCTCACTTTC
    ACTGTCCGCA GCTTTTCTTT TGCCGGATCT GAGCAGAAGA ATGGGGCCTG GATGGGAGAG
    GATGTGAGGT CCTCAATGAG TGCTGAAGGG CTGTCTTCTG TATGCCTGCT AGAAAAGAAA
    ACTCTTGTCA ATTCAGTCAG TGTGGCAGTG GAGGCCATTT TGGCTCAGTT CAGTTCATCC
    AGGACTGTTG TTCAGAAGAG CCTTTCTGGA GACAGCAGTG TAAATCCCTC TCTTGGTCGT
    CTGGTACTGC AGTGCCTTTG CCCAGCCCTG CGCAGCCTGC TCTGTGATGG ACTCAAGCCC
    CACCAGAGTG ACCTCATTGC AGGCAGAAGG CCAAATTCAC CCTGGGGTTT GGTTCAGGCC
    TCCACCAAAC CAGGCCCGAG CACACAGGCT TTGCACAGCC TTCAGACTAA AGTAGCAGGT
    CTTCATCAGC TAAGGCAGAG CAGGCACAGA TTCAACGCCT TCCTGTTTGG CCTTCTCAAT
    ATCAAGCTTT TGGATTATTG GCTTTCCCAT CTGCAGTCTT GCAGCGATGT GCTGGAAACT
    TTTTATCGGC CTACTTCCTT CATGCGTCTG TCACTCACTT CTTGTCAGTC TCTTTTTGAG
    GAGCTTCTTC TCCTGCTCCA GCCTCTTTCT CTGCTGACCT TCAACCTGGA CCTGCTGTTT
    CAGCATCATC ACCTAGACCC TTCTTCTCCA ACTCTCACCC CAGCCAGCCA CACGTCTGAC
    ATATGCAGCC CACCAAATGA AGACTTCAGC TTCCACCTGT CACCCAAGGG CCTTTTCCAT
    GGAGGTGTCC ATCGTACTGG AAGCATCTTA GAGCAAGACC ACAGTTTGAG TTTGGACACC
    AACCCTAATT CGTTTTTTTC GAGTCATGTT CTTGATGTGT CAGCATATCG CAATCCAATT
    TCTCTGTCAT CAGGCATCAA CAAAGAGGAA GCTGATCCGC CATTATCATG GTTTAACGGG
    AATGAGATCT CTGCACCTCT TAATGCTGGA AGTCTTTCCC AGCAAGCGGG CCAGGCTCTT
    CAGCAAGGCT GGGGGGCAGT CATGCGCTTG GGGGAGCGTT TAGGGCAGAA CTTTGGTTTG
    GCCACCACTA CAGAGAATGC GAATGGTCCT TACTCACCAG ATAATTCCAA GACAGGCTAC
    TCAGCAACAC CAAGAAGCCC AGTTGAAAAC AGGCAACAAC CTAGAGAGGA TCTTTCCCTG
    TGGGATAGTG GAGCTGCTAT TCCTTGGGGT CTTGGACGGC TCTTTGGTGC TTCTAAGAGC
    CCCAACAACC CACCGACCAC CAGGCGCCCA TCTCAGTGGC TCTCCCCTGG TGTATCCGTC
    CTTTCCCGGA TAGTGAATCT TAGCCAGGGT TCTCCACCTG AGAAAAGAGA GCTTCAGAGA
    AGCAATGATA AAGAGCAAGA TGAGAATGAA AATATTAATG AACCAAGAGA TCAGCCAAAG
    CCCCTAAGGG TGGTCAGAAC TCTGTGTGAT CACACTGGCA CTGGGGCCGA GCTCAGCTTC
    AGGAAGGGAG AGGAGCTTGT TCTGTTGGGG GGTGTCGACC ACGACTGGAT TCGATGTCGC
    CAAGGTCACC GAGAGGGCCT TGTTCCCATT GGTTATGCTT CACTTATCAT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa21247
    Clone ID Related Accession (Same CDS sequence) XM_005157912.4 , XM_005157912.3
    Accession Version XM_005157912.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5634bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product RUN and SH3 domain-containing protein 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005157912.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGCACCCCT CCTCTCGTCC CTCTGTGCCC CCACGTACCC GCAGGTTTGA CAATAGGCGC 
    ACAGAGCCAA AACCAAGTCA AGGGGCCAAG AGGGAGGACA AAAATATGAA CACAGGATCT
    TCATCCCCTC GTCGTGGCAC TCGAGGACCA CCCGAGTCTT CCAGGTTAAA AGGCCCTGGT
    CAAGGTGCAA GGGCCCCAGT GGTTCAGTCC AAACTGACTC GGCCAGTGAG GAATGGTGCT
    CACGCAATAC CAGCAGTCTC AAAACCAAAA AGTGGGCGCG CTGTATCAAA CCTAGCCCCA
    GCTGTGGACC CAAATTGCAA TGAGCCCAGC CTTCCTTGTC TGTGCTGTGA TGGCCATTCA
    CCTCAGGATA GCAACAGCCT TTTTAATCAC AACCATAACA ACAATAACAC TGTAAACATC
    AGGCAACAAA TGAAGATACC GCCACCATCT CCTCCGAATG AGTTGCCTTT TCCAAAACAA
    GAAACTGAAG ACGATAAGTG TGAGCCAGAA GTAAAACAAC CTGATGACTT ACCTTTCCCA
    GTGGAGGAGA AAGAGGAGGA AAATGGTAAT GTGGATAAAA AAGAGGGCGA TGACCTCAAA
    AATGATGACT GCAGTGTAAA CGTGATTGGC AATGTTGATG TGAATGGCAA TGGCAATTGT
    GATGGGGATG ATGATGAAGA TGACGATGAA GATGATGATG ATACTCTTGT TCCTTCTTGC
    TGCAATTGCC CAGAATCCAT GCTGGACTTC TCTCTCACAT CTTCTACCTC ATCTTCTTCC
    ACGTCCATTA GTAGCTGCTC AGATCTGGAG TGTGACTGCC CTGATACATT TTCATTGTCA
    TCCCAGGACC AAGAAGGCCC AGAATGTTAT CCATCTTCTT GTTCCCCACT CTCCAGCTGT
    CCTTCCTTTC AGTCCAATGC CATACCATTG GATCTCAACC TTTCTGCAAG ATCACCTTGC
    TCTTCTGATG AAGGCTATCC ATCAGCACCT TGCTCTCCTT CTGACTACAC AGAGGGCAAG
    TTTTCAGAAG AAGAAAAATG TATTAAAGTG GATCTTCTCC ATTTTTTAGA CTCCATAGAA
    GAGTTGGGTA AAATGGATTT GTTCTACCGC ATTGTTAGGC TGGCACATTG GCAACTTGAT
    GGAGAACTGA TTCTCAGAGA TAGGTTGGAC CACCAGCAGA AGCTTCAAAG GGTCAACAAA
    GAAGTGAAGT TGGCTTATTT TGTGAAACTT CAAGAAGAAG GGTTCAACTT TGGCGATGAG
    GATATCACTG GAGTTTTGGA CGAAATGGGC AACATTGACA TTCCATGGAA GTTATATAAA
    AGCAACAAAT CAAGTGACTC CCAGGAATTT TCTGATGCTG GGGTGGATCT GACAGCTCCT
    TCTGACCTTG ATGAAACTGC TTCCTCTGAT TCACTTGCTC CATCACCATT AGAGCCTCCT
    CCCCGTCCCC CAAAGCCTCC AACAAGGCAT GTGAGTGTTC AGCAGGGATC ACACACTTAT
    GAGAACATCA GTGGGCATAC CTTCTCAGCC CCATCCACCA ATAATGCTAC TTCTTTCTCC
    ACAAGTGTCC CTGTTCCTTC CATGGCATCT ACATCCCCAG CCTTGAAACC TCCTGCCCTT
    CCGCCTCCAC CATCACAGCC TGTGCCCTAC TTCTCTCTTA ATTCAGACAG ACCTGCTCAC
    TCCTCCCCCA CACCACCCAT CCCTCCACCA AGAAGACGTC ATAAAGCCCG ACTAGAGGCT
    CAAAGACTTG CTGAACTTGA AAAAGAAAAG ACTCCTCTAT CCCTTCCACC TCCAACGTCA
    AGACCTCCTC CGTTGCCACC ACCACCTGCT ATGTCCTCTC CCCCAGCTAT CCCACCTCCA
    CCATCAATCC CACCGCCTCC ATCTTTTCAT GCTCTGGATG TGGAAATCCG AAAGTTGCTT
    GCACTAGCAG GACTCACCCA AGCTGAATTG CTTAAACTTA GTCCTGAATT GGGTGTTTGT
    GTAGACGTAG TTGTGGATAA TGAACAGCAT CCAATGTCTG ATGTCTCACA AATTGGAGAA
    ATCAGTGCAA ACAGAGCACA CAAGGAAGAC AGTATGGATA AGCGGTTACA TTGCAGATTG
    AGGGAAGAAA CCAGATTTGG GAAAGATATA CAGAAGGTAG AAACTCTAAG TGAAAACGAG
    ATTTCTAAAG ACGGGCAGAA AGAAGCTTAC AGGACAACAT CCTTCACAGA AATGGCAAGA
    CGACGAAAGA GAAATAGTGG AAACTGTAAC CAAAACTGCA ACTGTGGTTT TGGATCTCCC
    AACTTGTATT ACAGCACTGA TCTTAGCAAT ACAAGCATCC CAAATGTTAG CTTTGGAAGT
    TTTGATTATA CTTCAGTGAT TTCAGACACC CCACCTCCTC CACCTCCACG ACCATTACCC
    CCTTGCCCAC CAGTTATTTC CAATCCTCCT GAACTTCCCC ATCTCAAGCC ATGCACTCTA
    CCTGCCAATG CATCACGACC TGATCGATTT GATTGGCTGA TAGCCTTTAC CCCAGATATT
    GAGCCACCAC CTACTGATAT GCGAAAACCA TCCAATGATG GCATGTTGCC AAAAGGCTCA
    ATTTCAGGCC CAAAAGTCAC CACTTTTAAA GAATTACGCA ATAGAAGCAA ACAGAGCTCC
    CCACCAGCTC CTGTTCAACC AGAACCTGAT CCAACAGTCA TCACTCCAGA CCCTGATTTC
    TTGTACAACC TCAAATGGAG AAGAGAGAAG ACAGATGGAG ATGGCTGGGA ATATACTTCC
    CAAGCACAAG CCTCTTTTCT TCAGCCTCCG CCAACTCCTG CCTCATTGTC CCTCTTTAGG
    GAAATGTGCC GCCTGAATAT ACAAGATGAA TGCAGAGCCG ATCCTAAAGT CTCCCAACAA
    ATGGGCTCAT CAGTAAGTGA AGGCAGCCTT AGGACTATTT GTGGTGATAA AGCTGCTCAC
    ACCAAAAAGA TTCAAAATGA AGATGACCTG GAGGTCAGGG GAATGGCAGA TGGAGCATGG
    ACTTTGGAGT CCAGGACAAC AGTGTCCTCA CCACCCCTGT CCCTTTCCTT GACCTCACCT
    TATTACCTGC ACTCTGCTTC CCTGTCACAA GCCTCCCAAT TTTATCACAC TGACACTACC
    AGTGATCACG CCATGTCCTA TTATGAGGGT TCCGTAAACA TCTCGAATCC CTTATGCATA
    AATTCCACTT TTGACTGCAG CAAACACGAC TATACTGACT CCCTGTACTC CGCCGGCCCG
    GAAAACAATA CATTTTTGTT CTGTAAACAA GAAAATGGTC TCAGTGGTGG CATGGATTCT
    CAGTATCACC ACTGTTATCA TGCTGACAGC TTTCTAGATT CTTTATACGT CTGTGGGCAT
    GAGCCTGGTA GTGCAAAAAA CAGCTTCTGC CCTGTGTATA ACAATGAAGT TGATTCAATC
    AGAAATGACG GTTCACTCTA CAGTGATAGT AGATACTTAG GAAACCCCCT GAAGCTGTTC
    ACTGACCCTG AACCACCGAC TGACATTAAC ACACTCACTG AAGGAGACAT AACACCTTAC
    AAAAACATCG ACATGCAGAC TTACATAACA ATGCGAAACC TTAAAAATAA GAGCAATGCG
    AAAAAATCCA CTTATAAAGA TGCCGACTCG CTTTTTGAGT TGAACTCGGG CAACATTAAT
    GATTCAAAAC CGTCTAACAA TCCATTTTTT GACAGTGGTG AACCCACAAA ACAGGACAAA
    CTTGTATCTT TCCCTGCCTA CTACCTTTAT AACCCTAAAA ACTGTCCCTT GCATAAAGGC
    GCCCCACCAC GGCTTTCCCC CGTAGGTGCG ATCTCTCCTC CCCACAGGTC CGGGCCTCCA
    GTTCCAGGCA CAGATGTTGC CCGTCTCTGT TCACCCCTTT TCCCTCGCAG TCACACCATT
    CCCGCCCTCG CTGCTCCCCT TTATTACCCT TATCTGTACA CCCCCCCTGT TGAAGCTCCT
    GTGCGGGAGC CACCGGTCCC CATACTTCAC TCTCTGCAGA GTCCACCACC GCTCACTTTC
    ACTGTCCGCA GCTTTTCTTT TGCCGGATCT GAGCAGAAGA ATGGGGCCTG GATGGGAGAG
    GATGTGAGGT CCTCAATGAG TGCTGAAGGG CTGTCTTCTG TATGCCTGCT AGAAAAGAAA
    ACTCTTGTCA ATTCAGTCAG TGTGGCAGTG GAGGCCATTT TGGCTCAGTT CAGTTCATCC
    AGGACTGTTG TTCAGAAGAG CCTTTCTGGA GACAGCAGTG TAAATCCCTC TCTTGGTCGT
    CTGGTACTGC AGTGCCTTTG CCCAGCCCTG CGCAGCCTGC TCTGTGATGG ACTCAAGCCC
    CACCAGAGTG ACCTCATTGC AGGCAGAAGG CCAAATTCAC CCTGGGGTTT GGTTCAGGCC
    TCCACCAAAC CAGGCCCGAG CACACAGGCT TTGCACAGCC TTCAGACTAA AGTAGCAGGT
    CTTCATCAGC TAAGGCAGAG CAGGCACAGA TTCAACGCCT TCCTGTTTGG CCTTCTCAAT
    ATCAAGCTTT TGGATTATTG GCTTTCCCAT CTGCAGTCTT GCAGCGATGT GCTGGAAACT
    TTTTATCGGC CTACTTCCTT CATGCGTCTG TCACTCACTT CTTGTCAGTC TCTTTTTGAG
    GAGCTTCTTC TCCTGCTCCA GCCTCTTTCT CTGCTGACCT TCAACCTGGA CCTGCTGTTT
    CAGCATCATC ACCTAGACCC TTCTTCTCCA ACTCTCACCC CAGCCAGCCA CACGTCTGAC
    ATATGCAGCC CACCAAATGA AGACTTCAGC TTCCACCTGT CACCCAAGGG CCTTTTCCAT
    GGAGGTGTCC ATCGTACTGG AAGCATCTTA GAGCAAGACC ACAGTTTGAG TTTGGACACC
    AACCCTAATT CGTTTTTTTC GAGTCATGTT CTTGATGTGT CAGCATATCG CAATCCAATT
    TCTCTGTCAT CAGGCATCAA CAAAGAGGAA GCTGATCCGC CATTATCATG GTTTAACGGG
    AATGAGATCT CTGCACCTCT TAATGCTGGA AGTCTTTCCC AGCAAGCGGG CCAGGCTCTT
    CAGCAAGGCT GGGGGGCAGT CATGCGCTTG GGGGAGCGTT TAGGGCAGAA CTTTGGTTTG
    GCCACCACTA CAGAGAATGC GAATGGTCCT TACTCACCAG ATAATTCCAA GACAGGCTAC
    TCAGCAACAC CAAGAAGCCC AGTTGAAAAC AGGCAACAAC CTAGAGAGGA TCTTTCCCTG
    TGGGATAGTG GAGCTGCTAT TCCTTGGGGT CTTGGACGGC TCTTTGGTGC TTCTAAGAGC
    CCCAACAACC CACCGACCAC CAGGCGCCCA TCTCAGTGGC TCTCCCCTGG TGTATCCGTC
    CTTTCCCGGA TAGTGAATCT TAGCCAGGGT TCTCCACCTG AGAAAAGAGA GCTTCAGAGA
    AGCAATGATA AAGAGCAAGA TGAGAATGAA AATATTAATG AACCAAGAGA TCAGCCAAAG
    CCCCTAAGGG TGGTCAGAAC TCTGTGTGAT CACACTGGCA CTGGGGCCGA GCTCAGCTTC
    AGGAAGGGAG AGGAGCTTGT TCTGTTGGGG GGTGTCGACC ACGACTGGAT TCGATGTCGC
    CAAGGTCACC GAGAGGGCCT TGTTCCCATT GGTTATGCTT CACTTATCAT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005157969.1
    CDS297..5930
    Translation

    Target ORF information:

    RefSeq Version XM_005157912.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio RUN and SH3 domain containing 1 (rusc1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005157912.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGCACCCCT CCTCTCGTCC CTCTGTGCCC CCACGTACCC GCAGGTTTGA CAATAGGCGC 
    ACAGAGCCAA AACCAAGTCA AGGGGCCAAG AGGGAGGACA AAAATATGAA CACAGGATCT
    TCATCCCCTC GTCGTGGCAC TCGAGGACCA CCCGAGTCTT CCAGGTTAAA AGGCCCTGGT
    CAAGGTGCAA GGGCCCCAGT GGTTCAGTCC AAACTGACTC GGCCAGTGAG GAATGGTGCT
    CACGCAATAC CAGCAGTCTC AAAACCAAAA AGTGGGCGCG CTGTATCAAA CCTAGCCCCA
    GCTGTGGACC CAAATTGCAA TGAGCCCAGC CTTCCTTGTC TGTGCTGTGA TGGCCATTCA
    CCTCAGGATA GCAACAGCCT TTTTAATCAC AACCATAACA ACAATAACAC TGTAAACATC
    AGGCAACAAA TGAAGATACC GCCACCATCT CCTCCGAATG AGTTGCCTTT TCCAAAACAA
    GAAACTGAAG ACGATAAGTG TGAGCCAGAA GTAAAACAAC CTGATGACTT ACCTTTCCCA
    GTGGAGGAGA AAGAGGAGGA AAATGGTAAT GTGGATAAAA AAGAGGGCGA TGACCTCAAA
    AATGATGACT GCAGTGTAAA CGTGATTGGC AATGTTGATG TGAATGGCAA TGGCAATTGT
    GATGGGGATG ATGATGAAGA TGACGATGAA GATGATGATG ATACTCTTGT TCCTTCTTGC
    TGCAATTGCC CAGAATCCAT GCTGGACTTC TCTCTCACAT CTTCTACCTC ATCTTCTTCC
    ACGTCCATTA GTAGCTGCTC AGATCTGGAG TGTGACTGCC CTGATACATT TTCATTGTCA
    TCCCAGGACC AAGAAGGCCC AGAATGTTAT CCATCTTCTT GTTCCCCACT CTCCAGCTGT
    CCTTCCTTTC AGTCCAATGC CATACCATTG GATCTCAACC TTTCTGCAAG ATCACCTTGC
    TCTTCTGATG AAGGCTATCC ATCAGCACCT TGCTCTCCTT CTGACTACAC AGAGGGCAAG
    TTTTCAGAAG AAGAAAAATG TATTAAAGTG GATCTTCTCC ATTTTTTAGA CTCCATAGAA
    GAGTTGGGTA AAATGGATTT GTTCTACCGC ATTGTTAGGC TGGCACATTG GCAACTTGAT
    GGAGAACTGA TTCTCAGAGA TAGGTTGGAC CACCAGCAGA AGCTTCAAAG GGTCAACAAA
    GAAGTGAAGT TGGCTTATTT TGTGAAACTT CAAGAAGAAG GGTTCAACTT TGGCGATGAG
    GATATCACTG GAGTTTTGGA CGAAATGGGC AACATTGACA TTCCATGGAA GTTATATAAA
    AGCAACAAAT CAAGTGACTC CCAGGAATTT TCTGATGCTG GGGTGGATCT GACAGCTCCT
    TCTGACCTTG ATGAAACTGC TTCCTCTGAT TCACTTGCTC CATCACCATT AGAGCCTCCT
    CCCCGTCCCC CAAAGCCTCC AACAAGGCAT GTGAGTGTTC AGCAGGGATC ACACACTTAT
    GAGAACATCA GTGGGCATAC CTTCTCAGCC CCATCCACCA ATAATGCTAC TTCTTTCTCC
    ACAAGTGTCC CTGTTCCTTC CATGGCATCT ACATCCCCAG CCTTGAAACC TCCTGCCCTT
    CCGCCTCCAC CATCACAGCC TGTGCCCTAC TTCTCTCTTA ATTCAGACAG ACCTGCTCAC
    TCCTCCCCCA CACCACCCAT CCCTCCACCA AGAAGACGTC ATAAAGCCCG ACTAGAGGCT
    CAAAGACTTG CTGAACTTGA AAAAGAAAAG ACTCCTCTAT CCCTTCCACC TCCAACGTCA
    AGACCTCCTC CGTTGCCACC ACCACCTGCT ATGTCCTCTC CCCCAGCTAT CCCACCTCCA
    CCATCAATCC CACCGCCTCC ATCTTTTCAT GCTCTGGATG TGGAAATCCG AAAGTTGCTT
    GCACTAGCAG GACTCACCCA AGCTGAATTG CTTAAACTTA GTCCTGAATT GGGTGTTTGT
    GTAGACGTAG TTGTGGATAA TGAACAGCAT CCAATGTCTG ATGTCTCACA AATTGGAGAA
    ATCAGTGCAA ACAGAGCACA CAAGGAAGAC AGTATGGATA AGCGGTTACA TTGCAGATTG
    AGGGAAGAAA CCAGATTTGG GAAAGATATA CAGAAGGTAG AAACTCTAAG TGAAAACGAG
    ATTTCTAAAG ACGGGCAGAA AGAAGCTTAC AGGACAACAT CCTTCACAGA AATGGCAAGA
    CGACGAAAGA GAAATAGTGG AAACTGTAAC CAAAACTGCA ACTGTGGTTT TGGATCTCCC
    AACTTGTATT ACAGCACTGA TCTTAGCAAT ACAAGCATCC CAAATGTTAG CTTTGGAAGT
    TTTGATTATA CTTCAGTGAT TTCAGACACC CCACCTCCTC CACCTCCACG ACCATTACCC
    CCTTGCCCAC CAGTTATTTC CAATCCTCCT GAACTTCCCC ATCTCAAGCC ATGCACTCTA
    CCTGCCAATG CATCACGACC TGATCGATTT GATTGGCTGA TAGCCTTTAC CCCAGATATT
    GAGCCACCAC CTACTGATAT GCGAAAACCA TCCAATGATG GCATGTTGCC AAAAGGCTCA
    ATTTCAGGCC CAAAAGTCAC CACTTTTAAA GAATTACGCA ATAGAAGCAA ACAGAGCTCC
    CCACCAGCTC CTGTTCAACC AGAACCTGAT CCAACAGTCA TCACTCCAGA CCCTGATTTC
    TTGTACAACC TCAAATGGAG AAGAGAGAAG ACAGATGGAG ATGGCTGGGA ATATACTTCC
    CAAGCACAAG CCTCTTTTCT TCAGCCTCCG CCAACTCCTG CCTCATTGTC CCTCTTTAGG
    GAAATGTGCC GCCTGAATAT ACAAGATGAA TGCAGAGCCG ATCCTAAAGT CTCCCAACAA
    ATGGGCTCAT CAGTAAGTGA AGGCAGCCTT AGGACTATTT GTGGTGATAA AGCTGCTCAC
    ACCAAAAAGA TTCAAAATGA AGATGACCTG GAGGTCAGGG GAATGGCAGA TGGAGCATGG
    ACTTTGGAGT CCAGGACAAC AGTGTCCTCA CCACCCCTGT CCCTTTCCTT GACCTCACCT
    TATTACCTGC ACTCTGCTTC CCTGTCACAA GCCTCCCAAT TTTATCACAC TGACACTACC
    AGTGATCACG CCATGTCCTA TTATGAGGGT TCCGTAAACA TCTCGAATCC CTTATGCATA
    AATTCCACTT TTGACTGCAG CAAACACGAC TATACTGACT CCCTGTACTC CGCCGGCCCG
    GAAAACAATA CATTTTTGTT CTGTAAACAA GAAAATGGTC TCAGTGGTGG CATGGATTCT
    CAGTATCACC ACTGTTATCA TGCTGACAGC TTTCTAGATT CTTTATACGT CTGTGGGCAT
    GAGCCTGGTA GTGCAAAAAA CAGCTTCTGC CCTGTGTATA ACAATGAAGT TGATTCAATC
    AGAAATGACG GTTCACTCTA CAGTGATAGT AGATACTTAG GAAACCCCCT GAAGCTGTTC
    ACTGACCCTG AACCACCGAC TGACATTAAC ACACTCACTG AAGGAGACAT AACACCTTAC
    AAAAACATCG ACATGCAGAC TTACATAACA ATGCGAAACC TTAAAAATAA GAGCAATGCG
    AAAAAATCCA CTTATAAAGA TGCCGACTCG CTTTTTGAGT TGAACTCGGG CAACATTAAT
    GATTCAAAAC CGTCTAACAA TCCATTTTTT GACAGTGGTG AACCCACAAA ACAGGACAAA
    CTTGTATCTT TCCCTGCCTA CTACCTTTAT AACCCTAAAA ACTGTCCCTT GCATAAAGGC
    GCCCCACCAC GGCTTTCCCC CGTAGGTGCG ATCTCTCCTC CCCACAGGTC CGGGCCTCCA
    GTTCCAGGCA CAGATGTTGC CCGTCTCTGT TCACCCCTTT TCCCTCGCAG TCACACCATT
    CCCGCCCTCG CTGCTCCCCT TTATTACCCT TATCTGTACA CCCCCCCTGT TGAAGCTCCT
    GTGCGGGAGC CACCGGTCCC CATACTTCAC TCTCTGCAGA GTCCACCACC GCTCACTTTC
    ACTGTCCGCA GCTTTTCTTT TGCCGGATCT GAGCAGAAGA ATGGGGCCTG GATGGGAGAG
    GATGTGAGGT CCTCAATGAG TGCTGAAGGG CTGTCTTCTG TATGCCTGCT AGAAAAGAAA
    ACTCTTGTCA ATTCAGTCAG TGTGGCAGTG GAGGCCATTT TGGCTCAGTT CAGTTCATCC
    AGGACTGTTG TTCAGAAGAG CCTTTCTGGA GACAGCAGTG TAAATCCCTC TCTTGGTCGT
    CTGGTACTGC AGTGCCTTTG CCCAGCCCTG CGCAGCCTGC TCTGTGATGG ACTCAAGCCC
    CACCAGAGTG ACCTCATTGC AGGCAGAAGG CCAAATTCAC CCTGGGGTTT GGTTCAGGCC
    TCCACCAAAC CAGGCCCGAG CACACAGGCT TTGCACAGCC TTCAGACTAA AGTAGCAGGT
    CTTCATCAGC TAAGGCAGAG CAGGCACAGA TTCAACGCCT TCCTGTTTGG CCTTCTCAAT
    ATCAAGCTTT TGGATTATTG GCTTTCCCAT CTGCAGTCTT GCAGCGATGT GCTGGAAACT
    TTTTATCGGC CTACTTCCTT CATGCGTCTG TCACTCACTT CTTGTCAGTC TCTTTTTGAG
    GAGCTTCTTC TCCTGCTCCA GCCTCTTTCT CTGCTGACCT TCAACCTGGA CCTGCTGTTT
    CAGCATCATC ACCTAGACCC TTCTTCTCCA ACTCTCACCC CAGCCAGCCA CACGTCTGAC
    ATATGCAGCC CACCAAATGA AGACTTCAGC TTCCACCTGT CACCCAAGGG CCTTTTCCAT
    GGAGGTGTCC ATCGTACTGG AAGCATCTTA GAGCAAGACC ACAGTTTGAG TTTGGACACC
    AACCCTAATT CGTTTTTTTC GAGTCATGTT CTTGATGTGT CAGCATATCG CAATCCAATT
    TCTCTGTCAT CAGGCATCAA CAAAGAGGAA GCTGATCCGC CATTATCATG GTTTAACGGG
    AATGAGATCT CTGCACCTCT TAATGCTGGA AGTCTTTCCC AGCAAGCGGG CCAGGCTCTT
    CAGCAAGGCT GGGGGGCAGT CATGCGCTTG GGGGAGCGTT TAGGGCAGAA CTTTGGTTTG
    GCCACCACTA CAGAGAATGC GAATGGTCCT TACTCACCAG ATAATTCCAA GACAGGCTAC
    TCAGCAACAC CAAGAAGCCC AGTTGAAAAC AGGCAACAAC CTAGAGAGGA TCTTTCCCTG
    TGGGATAGTG GAGCTGCTAT TCCTTGGGGT CTTGGACGGC TCTTTGGTGC TTCTAAGAGC
    CCCAACAACC CACCGACCAC CAGGCGCCCA TCTCAGTGGC TCTCCCCTGG TGTATCCGTC
    CTTTCCCGGA TAGTGAATCT TAGCCAGGGT TCTCCACCTG AGAAAAGAGA GCTTCAGAGA
    AGCAATGATA AAGAGCAAGA TGAGAATGAA AATATTAATG AACCAAGAGA TCAGCCAAAG
    CCCCTAAGGG TGGTCAGAAC TCTGTGTGAT CACACTGGCA CTGGGGCCGA GCTCAGCTTC
    AGGAAGGGAG AGGAGCTTGT TCTGTTGGGG GGTGTCGACC ACGACTGGAT TCGATGTCGC
    CAAGGTCACC GAGAGGGCCT TGTTCCCATT GGTTATGCTT CACTTATCAT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa21248
    Clone ID Related Accession (Same CDS sequence) XM_681985.8 , XM_681985.9
    Accession Version XM_681985.8 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4653bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product RUN and SH3 domain-containing protein 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_681985.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCACCCCT CCTCTCGTCC CTCTGTGCCC CCACGTACCC GCAGGTTTGA CAATAGGCGC 
    ACAGAGCCAA AACCAAGTCA AGGGGCCAAG AGGGAGGACA AAAATATGAA CACAGGATCT
    TCATCCCCTC GTCGTGGCAC TCGAGGACCA CCCGAGTCTT CCAGGTTAAA AGGCCCTGGT
    CAAGGTGCAA GGGCCCCAGT GGTTCAGTCC AAACTGACTC GGCCAGTGAG GAATGGTGCT
    CACGCAATAC CAGCAGTCTC AAAACCAAAA AGTGGGCGCG CTGTATCAAA CCTAGCCCCA
    GCTGTGGACC CAAATTGCAA TGAGCCCAGC CTTCCTTGTC TGTGCTGTGA TGGCCATTCA
    CCTCAGGATA GCAACAGCCT TTTTAATCAC AACCATAACA ACAATAACAC TGTAAACATC
    AGGCAACAAA TGAAGATACC GCCACCATCT CCTCCGAATG AGTTGCCTTT TCCAAAACAA
    GAAACTGAAG ACGATAAGTG TGAGCCAGAA GTAAAACAAC CTGATGACTT ACCTTTCCCA
    GTGGAGGAGA AAGAGGAGGA AAATGGTAAT GTGGATAAAA AAGAGGGCGA TGACCTCAAA
    AATGATGACT GCAGTGTAAA CGTGATTGGC AATGTTGATG TGAATGGCAA TGGCAATTGT
    GATGGGGATG ATGATGAAGA TGACGATGAA GATGATGATG ATACTCTTGT TCCTTCTTGC
    TGCAATTGCC CAGAATCCAT GCTGGACTTC TCTCTCACAT CTTCTACCTC ATCTTCTTCC
    ACGTCCATTA GTAGCTGCTC AGATCTGGAG TGTGACTGCC CTGATACATT TTCATTGTCA
    TCCCAGGACC AAGAAGGCCC AGAATGTTAT CCATCTTCTT GTTCCCCACT CTCCAGCTGT
    CCTTCCTTTC AGTCCAATGC CATACCATTG GATCTCAACC TTTCTGCAAG ATCACCTTGC
    TCTTCTGATG AAGGCTATCC ATCAGCACCT TGCTCTCCTT CTGACTACAC AGAGGGCAAG
    TTTTCAGAAG AAGAAAAATG TATTAAAGTG GATCTTCTCC ATTTTTTAGA CTCCATAGAA
    GAGTTGGGTA AAATGGATTT GTTCTACCGC ATTGTTAGGC TGGCACATTG GCAACTTGAT
    GGAGAACTGA TTCTCAGAGA TAGGTTGGAC CACCAGCAGA AGCTTCAAAG GGTCAACAAA
    GAAGTGAAGT TGGCTTATTT TGTGAAACTT CAAGAAGAAG GGTTCAACTT TGGCGATGAG
    GATATCACTG GAGTTTTGGA CGAAATGGGC AACATTGACA TTCCATGGAA GTTATATAAA
    AGCAACAAAT CAAGTGACTC CCAGGAATTT TCTGATGCTG GGGTGGATCT GACAGCTCCT
    TCTGACCTTG ATGAAACTGC TTCCTCTGAT TCACTTGCTC CATCACCATT AGAGCCTCCT
    CCCCGTCCCC CAAAGCCTCC AACAAGGCAT GTGAGTGTTC AGCAGGGATC ACACACTTAT
    GAGAACATCA GTGGGCATAC CTTCTCAGCC CCATCCACCA ATAATGCTAC TTCTTTCTCC
    ACAAGTGTCC CTGTTCCTTC CATGGCATCT ACATCCCCAG CCTTGAAACC TCCTGCCCTT
    CCGCCTCCAC CATCACAGCC TGTGCCCTAC TTCTCTCTTA ATTCAGACAG ACCTGCTCAC
    TCCTCCCCCA CACCACCCAT CCCTCCACCA AGAAGACGTC ATAAAGCCCG ACTAGAGGCT
    CAAAGACTTG CTGAACTTGA AAAAGAAAAG ACTCCTCTAT CCCTTCCACC TCCAACGTCA
    AGACCTCCTC CGTTGCCACC ACCACCTGCT ATGTCCTCTC CCCCAGCTAT CCCACCTCCA
    CCATCAATCC CACCGCCTCC ATCTTTTCAT GCTCTGGATG TGGAAATCCG AAAGTTGCTT
    GCACTAGCAG GACTCACCCA AGCTGAATTG CTTAAACTTA GTCCTGAATT GGGTGTTTGT
    GTAGACGTAG TTGTGGATAA TGAACAGCAT CCAATGTCTG ATGTCTCACA AATTGGAGAA
    ATCAGTGCAA ACAGAGCACA CAAGGAAGAC AGTATGGATA AGCGGTTACA TTGCAGATTG
    AGGGAAGAAA CCAGATTTGG GAAAGATATA CAGAAGGTAG AAACTCTAAG TGAAAACGAG
    ATTTCTAAAG ACGGGCAGAA AGAAGCTTAC AGGACAACAT CCTTCACAGA AATGGCAAGA
    CGACGAAAGA GAAATAGTGG AAACTGTAAC CAAAACTGCA ACTGTGGTTT TGGATCTCCC
    AACTTGTATT ACAGCACTGA TCTTAGCAAT ACAAGCATCC CAAATGTTAG CTTTGGAAGT
    TTTGATTATA CTTCAGTGAT TTCAGACACC CCACCTCCTC CACCTCCACG ACCATTACCC
    CCTTGCCCAC CAGTTATTTC CAATCCTCCT GAACTTCCCC ATCTCAAGCC ATGCACTCTA
    CCTGCCAATG CATCACGACC TGATCGATTT GATTGGCTGA TAGCCTTTAC CCCAGATATT
    GAGCCACCAC CTACTGATAT GCGAAAACCA TCCAATGATG GCATGTTGCC AAAAGGCTCA
    ATTTCAGGCC CAAAAGTCAC CACTTTTAAA GAATTACGCA ATAGAAGCAA ACAGAGCTCC
    CCACCAGCTC CTGTTCAACC AGAACCTGAT CCAACAGTCA TCACTCCAGA CCCTGATTTC
    TTGTACAACC TCAAATGGAG AAGAGAGAAG ACAGATGGAG ATGGCTGGGA ATATACTTCC
    CAAGCACAAG CCTCTTTTCT TCAGCCTCCG CCAACTCCTG CCTCATTGTC CCTCTTTAGG
    GAAATGTGCC GCCTGAATAT ACAAGATGAA TGCAGAGCCG ATCCTAAAGT CTCCCAACAA
    ATGGGCTCAT CAGTAAGTGA AGGCAGCCTT AGGACTATTT GTGGTGATAA AGCTGCTCAC
    ACCAAAAAGA TTCAAAATGA AGATGACCTG GAGGTCAGGG GAATGGCAGA TGGAGCATGG
    ACTTTGGAGT CCAGGACAAC AGCTGTCCGC AGCTTTTCTT TTGCCGGATC TGAGCAGAAG
    AATGGGGCCT GGATGGGAGA GGATGTGAGG TCCTCAATGA GTGCTGAAGG GCTGTCTTCT
    GTATGCCTGC TAGAAAAGAA AACTCTTGTC AATTCAGTCA GTGTGGCAGT GGAGGCCATT
    TTGGCTCAGT TCAGTTCATC CAGGACTGTT GTTCAGAAGT TTCACTCAGT AGATAAGAGC
    CTTTCTGGAG ACAGCAGTGT AAATCCCTCT CTTGGTCGTC TGGTACTGCA GTGCCTTTGC
    CCAGCCCTGC GCAGCCTGCT CTGTGATGGA CTCAAGCCCC ACCAGAGTGA CCTCATTGCA
    GGCAGAAGGC CAAATTCACC CTGGGGTTTG GTTCAGGCCT CCACCAAACC AGGCCCGAGC
    ACACAGGCTT TGCACAGCCT TCAGACTAAA GTAGCAGGTC TTCATCAGCT AAGGCAGAGC
    AGGCACAGAT TCAACGCCTT CCTGTTTGGC CTTCTCAATA TCAAGCTTTT GGATTATTGG
    CTTTCCCATC TGCAGTCTTG CAGCGATGTG CTGGAAACTT TTTATCGGCC TACTTCCTTC
    ATGCGTCTGT CACTCACTTC TTGTCAGTCT CTTTTTGAGG AGCTTCTTCT CCTGCTCCAG
    CCTCTTTCTC TGCTGACCTT CAACCTGGAC CTGCTGTTTC AGCATCATCA CCTAGACCCT
    TCTTCTCCAA CTCTCACCCC AGCCAGCCAC ACGTCTGACA TATGCAGCCC ACCAAATGAA
    GACTTCAGCT TCCACCTGTC ACCCAAGGGC CTTTTCCATG GAGGTGTCCA TCGTACTGGA
    AGCATCTTAG AGCAAGACCA CAGTTTGAGT TTGGACACCA ACCCTAATTC GTTTTTTTCG
    AGTCATGTTC TTGATGTGTC AGCATATCGC AATCCAATTT CTCTGTCATC AGGCATCAAC
    AAAGAGGAAG CTGATCCGCC ATTATCATGG TTTAACGGGA ATGAGATCTC TGCACCTCTT
    AATGCTGGAA GTCTTTCCCA GCAAGCGGGC CAGGCTCTTC AGCAAGGCTG GGGGGCAGTC
    ATGCGCTTGG GGGAGCGTTT AGGGCAGAAC TTTGGTTTGG CCACCACTAC AGAGAATGCG
    AATGGTCCTT ACTCACCAGA TAATTCCAAG ACAGGCTACT CAGCAACACC AAGAAGCCCA
    GTTGAAAACA GGCAACAACC TAGAGAGGAT CTTTCCCTGT GGGATAGTGG AGCTGCTATT
    CCTTGGGGTC TTGGACGGCT CTTTGGTGCT TCTAAGAGCC CCAACAACCC ACCGACCACC
    AGGCGCCCAT CTCAGTGGCT CTCCCCTGGT GTATCCGTCC TTTCCCGGAT AGTGAATCTT
    AGCCAGGGTT CTCCACCTGA GAAAAGAGAG CTTCAGAGAA GCAATGATAA AGAGCAAGAT
    GAGAATGAAA ATATTAATGA ACCAAGAGAT CAGCCAAAGC CCCTAAGGGT GGTCAGAACT
    CTGTGTGATC ACACTGGCAC TGGGGCCGAG CTCAGCTTCA GGAAGGGAGA GGAGCTTGTT
    CTGTTGGGGG GTGTCGACCA CGACTGGATT CGATGTCGCC AAGGTCACCG AGAGGGCCTT
    GTTCCCATTG GTTATGCTTC ACTTATCATG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_687077.5
    CDS295..4947
    Translation

    Target ORF information:

    RefSeq Version XM_681985.8
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio RUN and SH3 domain containing 1 (rusc1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_681985.8

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCACCCCT CCTCTCGTCC CTCTGTGCCC CCACGTACCC GCAGGTTTGA CAATAGGCGC 
    ACAGAGCCAA AACCAAGTCA AGGGGCCAAG AGGGAGGACA AAAATATGAA CACAGGATCT
    TCATCCCCTC GTCGTGGCAC TCGAGGACCA CCCGAGTCTT CCAGGTTAAA AGGCCCTGGT
    CAAGGTGCAA GGGCCCCAGT GGTTCAGTCC AAACTGACTC GGCCAGTGAG GAATGGTGCT
    CACGCAATAC CAGCAGTCTC AAAACCAAAA AGTGGGCGCG CTGTATCAAA CCTAGCCCCA
    GCTGTGGACC CAAATTGCAA TGAGCCCAGC CTTCCTTGTC TGTGCTGTGA TGGCCATTCA
    CCTCAGGATA GCAACAGCCT TTTTAATCAC AACCATAACA ACAATAACAC TGTAAACATC
    AGGCAACAAA TGAAGATACC GCCACCATCT CCTCCGAATG AGTTGCCTTT TCCAAAACAA
    GAAACTGAAG ACGATAAGTG TGAGCCAGAA GTAAAACAAC CTGATGACTT ACCTTTCCCA
    GTGGAGGAGA AAGAGGAGGA AAATGGTAAT GTGGATAAAA AAGAGGGCGA TGACCTCAAA
    AATGATGACT GCAGTGTAAA CGTGATTGGC AATGTTGATG TGAATGGCAA TGGCAATTGT
    GATGGGGATG ATGATGAAGA TGACGATGAA GATGATGATG ATACTCTTGT TCCTTCTTGC
    TGCAATTGCC CAGAATCCAT GCTGGACTTC TCTCTCACAT CTTCTACCTC ATCTTCTTCC
    ACGTCCATTA GTAGCTGCTC AGATCTGGAG TGTGACTGCC CTGATACATT TTCATTGTCA
    TCCCAGGACC AAGAAGGCCC AGAATGTTAT CCATCTTCTT GTTCCCCACT CTCCAGCTGT
    CCTTCCTTTC AGTCCAATGC CATACCATTG GATCTCAACC TTTCTGCAAG ATCACCTTGC
    TCTTCTGATG AAGGCTATCC ATCAGCACCT TGCTCTCCTT CTGACTACAC AGAGGGCAAG
    TTTTCAGAAG AAGAAAAATG TATTAAAGTG GATCTTCTCC ATTTTTTAGA CTCCATAGAA
    GAGTTGGGTA AAATGGATTT GTTCTACCGC ATTGTTAGGC TGGCACATTG GCAACTTGAT
    GGAGAACTGA TTCTCAGAGA TAGGTTGGAC CACCAGCAGA AGCTTCAAAG GGTCAACAAA
    GAAGTGAAGT TGGCTTATTT TGTGAAACTT CAAGAAGAAG GGTTCAACTT TGGCGATGAG
    GATATCACTG GAGTTTTGGA CGAAATGGGC AACATTGACA TTCCATGGAA GTTATATAAA
    AGCAACAAAT CAAGTGACTC CCAGGAATTT TCTGATGCTG GGGTGGATCT GACAGCTCCT
    TCTGACCTTG ATGAAACTGC TTCCTCTGAT TCACTTGCTC CATCACCATT AGAGCCTCCT
    CCCCGTCCCC CAAAGCCTCC AACAAGGCAT GTGAGTGTTC AGCAGGGATC ACACACTTAT
    GAGAACATCA GTGGGCATAC CTTCTCAGCC CCATCCACCA ATAATGCTAC TTCTTTCTCC
    ACAAGTGTCC CTGTTCCTTC CATGGCATCT ACATCCCCAG CCTTGAAACC TCCTGCCCTT
    CCGCCTCCAC CATCACAGCC TGTGCCCTAC TTCTCTCTTA ATTCAGACAG ACCTGCTCAC
    TCCTCCCCCA CACCACCCAT CCCTCCACCA AGAAGACGTC ATAAAGCCCG ACTAGAGGCT
    CAAAGACTTG CTGAACTTGA AAAAGAAAAG ACTCCTCTAT CCCTTCCACC TCCAACGTCA
    AGACCTCCTC CGTTGCCACC ACCACCTGCT ATGTCCTCTC CCCCAGCTAT CCCACCTCCA
    CCATCAATCC CACCGCCTCC ATCTTTTCAT GCTCTGGATG TGGAAATCCG AAAGTTGCTT
    GCACTAGCAG GACTCACCCA AGCTGAATTG CTTAAACTTA GTCCTGAATT GGGTGTTTGT
    GTAGACGTAG TTGTGGATAA TGAACAGCAT CCAATGTCTG ATGTCTCACA AATTGGAGAA
    ATCAGTGCAA ACAGAGCACA CAAGGAAGAC AGTATGGATA AGCGGTTACA TTGCAGATTG
    AGGGAAGAAA CCAGATTTGG GAAAGATATA CAGAAGGTAG AAACTCTAAG TGAAAACGAG
    ATTTCTAAAG ACGGGCAGAA AGAAGCTTAC AGGACAACAT CCTTCACAGA AATGGCAAGA
    CGACGAAAGA GAAATAGTGG AAACTGTAAC CAAAACTGCA ACTGTGGTTT TGGATCTCCC
    AACTTGTATT ACAGCACTGA TCTTAGCAAT ACAAGCATCC CAAATGTTAG CTTTGGAAGT
    TTTGATTATA CTTCAGTGAT TTCAGACACC CCACCTCCTC CACCTCCACG ACCATTACCC
    CCTTGCCCAC CAGTTATTTC CAATCCTCCT GAACTTCCCC ATCTCAAGCC ATGCACTCTA
    CCTGCCAATG CATCACGACC TGATCGATTT GATTGGCTGA TAGCCTTTAC CCCAGATATT
    GAGCCACCAC CTACTGATAT GCGAAAACCA TCCAATGATG GCATGTTGCC AAAAGGCTCA
    ATTTCAGGCC CAAAAGTCAC CACTTTTAAA GAATTACGCA ATAGAAGCAA ACAGAGCTCC
    CCACCAGCTC CTGTTCAACC AGAACCTGAT CCAACAGTCA TCACTCCAGA CCCTGATTTC
    TTGTACAACC TCAAATGGAG AAGAGAGAAG ACAGATGGAG ATGGCTGGGA ATATACTTCC
    CAAGCACAAG CCTCTTTTCT TCAGCCTCCG CCAACTCCTG CCTCATTGTC CCTCTTTAGG
    GAAATGTGCC GCCTGAATAT ACAAGATGAA TGCAGAGCCG ATCCTAAAGT CTCCCAACAA
    ATGGGCTCAT CAGTAAGTGA AGGCAGCCTT AGGACTATTT GTGGTGATAA AGCTGCTCAC
    ACCAAAAAGA TTCAAAATGA AGATGACCTG GAGGTCAGGG GAATGGCAGA TGGAGCATGG
    ACTTTGGAGT CCAGGACAAC AGCTGTCCGC AGCTTTTCTT TTGCCGGATC TGAGCAGAAG
    AATGGGGCCT GGATGGGAGA GGATGTGAGG TCCTCAATGA GTGCTGAAGG GCTGTCTTCT
    GTATGCCTGC TAGAAAAGAA AACTCTTGTC AATTCAGTCA GTGTGGCAGT GGAGGCCATT
    TTGGCTCAGT TCAGTTCATC CAGGACTGTT GTTCAGAAGT TTCACTCAGT AGATAAGAGC
    CTTTCTGGAG ACAGCAGTGT AAATCCCTCT CTTGGTCGTC TGGTACTGCA GTGCCTTTGC
    CCAGCCCTGC GCAGCCTGCT CTGTGATGGA CTCAAGCCCC ACCAGAGTGA CCTCATTGCA
    GGCAGAAGGC CAAATTCACC CTGGGGTTTG GTTCAGGCCT CCACCAAACC AGGCCCGAGC
    ACACAGGCTT TGCACAGCCT TCAGACTAAA GTAGCAGGTC TTCATCAGCT AAGGCAGAGC
    AGGCACAGAT TCAACGCCTT CCTGTTTGGC CTTCTCAATA TCAAGCTTTT GGATTATTGG
    CTTTCCCATC TGCAGTCTTG CAGCGATGTG CTGGAAACTT TTTATCGGCC TACTTCCTTC
    ATGCGTCTGT CACTCACTTC TTGTCAGTCT CTTTTTGAGG AGCTTCTTCT CCTGCTCCAG
    CCTCTTTCTC TGCTGACCTT CAACCTGGAC CTGCTGTTTC AGCATCATCA CCTAGACCCT
    TCTTCTCCAA CTCTCACCCC AGCCAGCCAC ACGTCTGACA TATGCAGCCC ACCAAATGAA
    GACTTCAGCT TCCACCTGTC ACCCAAGGGC CTTTTCCATG GAGGTGTCCA TCGTACTGGA
    AGCATCTTAG AGCAAGACCA CAGTTTGAGT TTGGACACCA ACCCTAATTC GTTTTTTTCG
    AGTCATGTTC TTGATGTGTC AGCATATCGC AATCCAATTT CTCTGTCATC AGGCATCAAC
    AAAGAGGAAG CTGATCCGCC ATTATCATGG TTTAACGGGA ATGAGATCTC TGCACCTCTT
    AATGCTGGAA GTCTTTCCCA GCAAGCGGGC CAGGCTCTTC AGCAAGGCTG GGGGGCAGTC
    ATGCGCTTGG GGGAGCGTTT AGGGCAGAAC TTTGGTTTGG CCACCACTAC AGAGAATGCG
    AATGGTCCTT ACTCACCAGA TAATTCCAAG ACAGGCTACT CAGCAACACC AAGAAGCCCA
    GTTGAAAACA GGCAACAACC TAGAGAGGAT CTTTCCCTGT GGGATAGTGG AGCTGCTATT
    CCTTGGGGTC TTGGACGGCT CTTTGGTGCT TCTAAGAGCC CCAACAACCC ACCGACCACC
    AGGCGCCCAT CTCAGTGGCT CTCCCCTGGT GTATCCGTCC TTTCCCGGAT AGTGAATCTT
    AGCCAGGGTT CTCCACCTGA GAAAAGAGAG CTTCAGAGAA GCAATGATAA AGAGCAAGAT
    GAGAATGAAA ATATTAATGA ACCAAGAGAT CAGCCAAAGC CCCTAAGGGT GGTCAGAACT
    CTGTGTGATC ACACTGGCAC TGGGGCCGAG CTCAGCTTCA GGAAGGGAGA GGAGCTTGTT
    CTGTTGGGGG GTGTCGACCA CGACTGGATT CGATGTCGCC AAGGTCACCG AGAGGGCCTT
    GTTCCCATTG GTTATGCTTC ACTTATCATG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa21248
    Clone ID Related Accession (Same CDS sequence) XM_681985.8 , XM_681985.9
    Accession Version XM_681985.9 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4653bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product RUN and SH3 domain-containing protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_681985.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCACCCCT CCTCTCGTCC CTCTGTGCCC CCACGTACCC GCAGGTTTGA CAATAGGCGC 
    ACAGAGCCAA AACCAAGTCA AGGGGCCAAG AGGGAGGACA AAAATATGAA CACAGGATCT
    TCATCCCCTC GTCGTGGCAC TCGAGGACCA CCCGAGTCTT CCAGGTTAAA AGGCCCTGGT
    CAAGGTGCAA GGGCCCCAGT GGTTCAGTCC AAACTGACTC GGCCAGTGAG GAATGGTGCT
    CACGCAATAC CAGCAGTCTC AAAACCAAAA AGTGGGCGCG CTGTATCAAA CCTAGCCCCA
    GCTGTGGACC CAAATTGCAA TGAGCCCAGC CTTCCTTGTC TGTGCTGTGA TGGCCATTCA
    CCTCAGGATA GCAACAGCCT TTTTAATCAC AACCATAACA ACAATAACAC TGTAAACATC
    AGGCAACAAA TGAAGATACC GCCACCATCT CCTCCGAATG AGTTGCCTTT TCCAAAACAA
    GAAACTGAAG ACGATAAGTG TGAGCCAGAA GTAAAACAAC CTGATGACTT ACCTTTCCCA
    GTGGAGGAGA AAGAGGAGGA AAATGGTAAT GTGGATAAAA AAGAGGGCGA TGACCTCAAA
    AATGATGACT GCAGTGTAAA CGTGATTGGC AATGTTGATG TGAATGGCAA TGGCAATTGT
    GATGGGGATG ATGATGAAGA TGACGATGAA GATGATGATG ATACTCTTGT TCCTTCTTGC
    TGCAATTGCC CAGAATCCAT GCTGGACTTC TCTCTCACAT CTTCTACCTC ATCTTCTTCC
    ACGTCCATTA GTAGCTGCTC AGATCTGGAG TGTGACTGCC CTGATACATT TTCATTGTCA
    TCCCAGGACC AAGAAGGCCC AGAATGTTAT CCATCTTCTT GTTCCCCACT CTCCAGCTGT
    CCTTCCTTTC AGTCCAATGC CATACCATTG GATCTCAACC TTTCTGCAAG ATCACCTTGC
    TCTTCTGATG AAGGCTATCC ATCAGCACCT TGCTCTCCTT CTGACTACAC AGAGGGCAAG
    TTTTCAGAAG AAGAAAAATG TATTAAAGTG GATCTTCTCC ATTTTTTAGA CTCCATAGAA
    GAGTTGGGTA AAATGGATTT GTTCTACCGC ATTGTTAGGC TGGCACATTG GCAACTTGAT
    GGAGAACTGA TTCTCAGAGA TAGGTTGGAC CACCAGCAGA AGCTTCAAAG GGTCAACAAA
    GAAGTGAAGT TGGCTTATTT TGTGAAACTT CAAGAAGAAG GGTTCAACTT TGGCGATGAG
    GATATCACTG GAGTTTTGGA CGAAATGGGC AACATTGACA TTCCATGGAA GTTATATAAA
    AGCAACAAAT CAAGTGACTC CCAGGAATTT TCTGATGCTG GGGTGGATCT GACAGCTCCT
    TCTGACCTTG ATGAAACTGC TTCCTCTGAT TCACTTGCTC CATCACCATT AGAGCCTCCT
    CCCCGTCCCC CAAAGCCTCC AACAAGGCAT GTGAGTGTTC AGCAGGGATC ACACACTTAT
    GAGAACATCA GTGGGCATAC CTTCTCAGCC CCATCCACCA ATAATGCTAC TTCTTTCTCC
    ACAAGTGTCC CTGTTCCTTC CATGGCATCT ACATCCCCAG CCTTGAAACC TCCTGCCCTT
    CCGCCTCCAC CATCACAGCC TGTGCCCTAC TTCTCTCTTA ATTCAGACAG ACCTGCTCAC
    TCCTCCCCCA CACCACCCAT CCCTCCACCA AGAAGACGTC ATAAAGCCCG ACTAGAGGCT
    CAAAGACTTG CTGAACTTGA AAAAGAAAAG ACTCCTCTAT CCCTTCCACC TCCAACGTCA
    AGACCTCCTC CGTTGCCACC ACCACCTGCT ATGTCCTCTC CCCCAGCTAT CCCACCTCCA
    CCATCAATCC CACCGCCTCC ATCTTTTCAT GCTCTGGATG TGGAAATCCG AAAGTTGCTT
    GCACTAGCAG GACTCACCCA AGCTGAATTG CTTAAACTTA GTCCTGAATT GGGTGTTTGT
    GTAGACGTAG TTGTGGATAA TGAACAGCAT CCAATGTCTG ATGTCTCACA AATTGGAGAA
    ATCAGTGCAA ACAGAGCACA CAAGGAAGAC AGTATGGATA AGCGGTTACA TTGCAGATTG
    AGGGAAGAAA CCAGATTTGG GAAAGATATA CAGAAGGTAG AAACTCTAAG TGAAAACGAG
    ATTTCTAAAG ACGGGCAGAA AGAAGCTTAC AGGACAACAT CCTTCACAGA AATGGCAAGA
    CGACGAAAGA GAAATAGTGG AAACTGTAAC CAAAACTGCA ACTGTGGTTT TGGATCTCCC
    AACTTGTATT ACAGCACTGA TCTTAGCAAT ACAAGCATCC CAAATGTTAG CTTTGGAAGT
    TTTGATTATA CTTCAGTGAT TTCAGACACC CCACCTCCTC CACCTCCACG ACCATTACCC
    CCTTGCCCAC CAGTTATTTC CAATCCTCCT GAACTTCCCC ATCTCAAGCC ATGCACTCTA
    CCTGCCAATG CATCACGACC TGATCGATTT GATTGGCTGA TAGCCTTTAC CCCAGATATT
    GAGCCACCAC CTACTGATAT GCGAAAACCA TCCAATGATG GCATGTTGCC AAAAGGCTCA
    ATTTCAGGCC CAAAAGTCAC CACTTTTAAA GAATTACGCA ATAGAAGCAA ACAGAGCTCC
    CCACCAGCTC CTGTTCAACC AGAACCTGAT CCAACAGTCA TCACTCCAGA CCCTGATTTC
    TTGTACAACC TCAAATGGAG AAGAGAGAAG ACAGATGGAG ATGGCTGGGA ATATACTTCC
    CAAGCACAAG CCTCTTTTCT TCAGCCTCCG CCAACTCCTG CCTCATTGTC CCTCTTTAGG
    GAAATGTGCC GCCTGAATAT ACAAGATGAA TGCAGAGCCG ATCCTAAAGT CTCCCAACAA
    ATGGGCTCAT CAGTAAGTGA AGGCAGCCTT AGGACTATTT GTGGTGATAA AGCTGCTCAC
    ACCAAAAAGA TTCAAAATGA AGATGACCTG GAGGTCAGGG GAATGGCAGA TGGAGCATGG
    ACTTTGGAGT CCAGGACAAC AGCTGTCCGC AGCTTTTCTT TTGCCGGATC TGAGCAGAAG
    AATGGGGCCT GGATGGGAGA GGATGTGAGG TCCTCAATGA GTGCTGAAGG GCTGTCTTCT
    GTATGCCTGC TAGAAAAGAA AACTCTTGTC AATTCAGTCA GTGTGGCAGT GGAGGCCATT
    TTGGCTCAGT TCAGTTCATC CAGGACTGTT GTTCAGAAGT TTCACTCAGT AGATAAGAGC
    CTTTCTGGAG ACAGCAGTGT AAATCCCTCT CTTGGTCGTC TGGTACTGCA GTGCCTTTGC
    CCAGCCCTGC GCAGCCTGCT CTGTGATGGA CTCAAGCCCC ACCAGAGTGA CCTCATTGCA
    GGCAGAAGGC CAAATTCACC CTGGGGTTTG GTTCAGGCCT CCACCAAACC AGGCCCGAGC
    ACACAGGCTT TGCACAGCCT TCAGACTAAA GTAGCAGGTC TTCATCAGCT AAGGCAGAGC
    AGGCACAGAT TCAACGCCTT CCTGTTTGGC CTTCTCAATA TCAAGCTTTT GGATTATTGG
    CTTTCCCATC TGCAGTCTTG CAGCGATGTG CTGGAAACTT TTTATCGGCC TACTTCCTTC
    ATGCGTCTGT CACTCACTTC TTGTCAGTCT CTTTTTGAGG AGCTTCTTCT CCTGCTCCAG
    CCTCTTTCTC TGCTGACCTT CAACCTGGAC CTGCTGTTTC AGCATCATCA CCTAGACCCT
    TCTTCTCCAA CTCTCACCCC AGCCAGCCAC ACGTCTGACA TATGCAGCCC ACCAAATGAA
    GACTTCAGCT TCCACCTGTC ACCCAAGGGC CTTTTCCATG GAGGTGTCCA TCGTACTGGA
    AGCATCTTAG AGCAAGACCA CAGTTTGAGT TTGGACACCA ACCCTAATTC GTTTTTTTCG
    AGTCATGTTC TTGATGTGTC AGCATATCGC AATCCAATTT CTCTGTCATC AGGCATCAAC
    AAAGAGGAAG CTGATCCGCC ATTATCATGG TTTAACGGGA ATGAGATCTC TGCACCTCTT
    AATGCTGGAA GTCTTTCCCA GCAAGCGGGC CAGGCTCTTC AGCAAGGCTG GGGGGCAGTC
    ATGCGCTTGG GGGAGCGTTT AGGGCAGAAC TTTGGTTTGG CCACCACTAC AGAGAATGCG
    AATGGTCCTT ACTCACCAGA TAATTCCAAG ACAGGCTACT CAGCAACACC AAGAAGCCCA
    GTTGAAAACA GGCAACAACC TAGAGAGGAT CTTTCCCTGT GGGATAGTGG AGCTGCTATT
    CCTTGGGGTC TTGGACGGCT CTTTGGTGCT TCTAAGAGCC CCAACAACCC ACCGACCACC
    AGGCGCCCAT CTCAGTGGCT CTCCCCTGGT GTATCCGTCC TTTCCCGGAT AGTGAATCTT
    AGCCAGGGTT CTCCACCTGA GAAAAGAGAG CTTCAGAGAA GCAATGATAA AGAGCAAGAT
    GAGAATGAAA ATATTAATGA ACCAAGAGAT CAGCCAAAGC CCCTAAGGGT GGTCAGAACT
    CTGTGTGATC ACACTGGCAC TGGGGCCGAG CTCAGCTTCA GGAAGGGAGA GGAGCTTGTT
    CTGTTGGGGG GTGTCGACCA CGACTGGATT CGATGTCGCC AAGGTCACCG AGAGGGCCTT
    GTTCCCATTG GTTATGCTTC ACTTATCATG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_687077.5
    CDS279..4931
    Translation

    Target ORF information:

    RefSeq Version XM_681985.9
    Organism Danio rerio(zebrafish)
    Definition Danio rerio RUN and SH3 domain containing 1 (rusc1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_681985.9

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCACCCCT CCTCTCGTCC CTCTGTGCCC CCACGTACCC GCAGGTTTGA CAATAGGCGC 
    ACAGAGCCAA AACCAAGTCA AGGGGCCAAG AGGGAGGACA AAAATATGAA CACAGGATCT
    TCATCCCCTC GTCGTGGCAC TCGAGGACCA CCCGAGTCTT CCAGGTTAAA AGGCCCTGGT
    CAAGGTGCAA GGGCCCCAGT GGTTCAGTCC AAACTGACTC GGCCAGTGAG GAATGGTGCT
    CACGCAATAC CAGCAGTCTC AAAACCAAAA AGTGGGCGCG CTGTATCAAA CCTAGCCCCA
    GCTGTGGACC CAAATTGCAA TGAGCCCAGC CTTCCTTGTC TGTGCTGTGA TGGCCATTCA
    CCTCAGGATA GCAACAGCCT TTTTAATCAC AACCATAACA ACAATAACAC TGTAAACATC
    AGGCAACAAA TGAAGATACC GCCACCATCT CCTCCGAATG AGTTGCCTTT TCCAAAACAA
    GAAACTGAAG ACGATAAGTG TGAGCCAGAA GTAAAACAAC CTGATGACTT ACCTTTCCCA
    GTGGAGGAGA AAGAGGAGGA AAATGGTAAT GTGGATAAAA AAGAGGGCGA TGACCTCAAA
    AATGATGACT GCAGTGTAAA CGTGATTGGC AATGTTGATG TGAATGGCAA TGGCAATTGT
    GATGGGGATG ATGATGAAGA TGACGATGAA GATGATGATG ATACTCTTGT TCCTTCTTGC
    TGCAATTGCC CAGAATCCAT GCTGGACTTC TCTCTCACAT CTTCTACCTC ATCTTCTTCC
    ACGTCCATTA GTAGCTGCTC AGATCTGGAG TGTGACTGCC CTGATACATT TTCATTGTCA
    TCCCAGGACC AAGAAGGCCC AGAATGTTAT CCATCTTCTT GTTCCCCACT CTCCAGCTGT
    CCTTCCTTTC AGTCCAATGC CATACCATTG GATCTCAACC TTTCTGCAAG ATCACCTTGC
    TCTTCTGATG AAGGCTATCC ATCAGCACCT TGCTCTCCTT CTGACTACAC AGAGGGCAAG
    TTTTCAGAAG AAGAAAAATG TATTAAAGTG GATCTTCTCC ATTTTTTAGA CTCCATAGAA
    GAGTTGGGTA AAATGGATTT GTTCTACCGC ATTGTTAGGC TGGCACATTG GCAACTTGAT
    GGAGAACTGA TTCTCAGAGA TAGGTTGGAC CACCAGCAGA AGCTTCAAAG GGTCAACAAA
    GAAGTGAAGT TGGCTTATTT TGTGAAACTT CAAGAAGAAG GGTTCAACTT TGGCGATGAG
    GATATCACTG GAGTTTTGGA CGAAATGGGC AACATTGACA TTCCATGGAA GTTATATAAA
    AGCAACAAAT CAAGTGACTC CCAGGAATTT TCTGATGCTG GGGTGGATCT GACAGCTCCT
    TCTGACCTTG ATGAAACTGC TTCCTCTGAT TCACTTGCTC CATCACCATT AGAGCCTCCT
    CCCCGTCCCC CAAAGCCTCC AACAAGGCAT GTGAGTGTTC AGCAGGGATC ACACACTTAT
    GAGAACATCA GTGGGCATAC CTTCTCAGCC CCATCCACCA ATAATGCTAC TTCTTTCTCC
    ACAAGTGTCC CTGTTCCTTC CATGGCATCT ACATCCCCAG CCTTGAAACC TCCTGCCCTT
    CCGCCTCCAC CATCACAGCC TGTGCCCTAC TTCTCTCTTA ATTCAGACAG ACCTGCTCAC
    TCCTCCCCCA CACCACCCAT CCCTCCACCA AGAAGACGTC ATAAAGCCCG ACTAGAGGCT
    CAAAGACTTG CTGAACTTGA AAAAGAAAAG ACTCCTCTAT CCCTTCCACC TCCAACGTCA
    AGACCTCCTC CGTTGCCACC ACCACCTGCT ATGTCCTCTC CCCCAGCTAT CCCACCTCCA
    CCATCAATCC CACCGCCTCC ATCTTTTCAT GCTCTGGATG TGGAAATCCG AAAGTTGCTT
    GCACTAGCAG GACTCACCCA AGCTGAATTG CTTAAACTTA GTCCTGAATT GGGTGTTTGT
    GTAGACGTAG TTGTGGATAA TGAACAGCAT CCAATGTCTG ATGTCTCACA AATTGGAGAA
    ATCAGTGCAA ACAGAGCACA CAAGGAAGAC AGTATGGATA AGCGGTTACA TTGCAGATTG
    AGGGAAGAAA CCAGATTTGG GAAAGATATA CAGAAGGTAG AAACTCTAAG TGAAAACGAG
    ATTTCTAAAG ACGGGCAGAA AGAAGCTTAC AGGACAACAT CCTTCACAGA AATGGCAAGA
    CGACGAAAGA GAAATAGTGG AAACTGTAAC CAAAACTGCA ACTGTGGTTT TGGATCTCCC
    AACTTGTATT ACAGCACTGA TCTTAGCAAT ACAAGCATCC CAAATGTTAG CTTTGGAAGT
    TTTGATTATA CTTCAGTGAT TTCAGACACC CCACCTCCTC CACCTCCACG ACCATTACCC
    CCTTGCCCAC CAGTTATTTC CAATCCTCCT GAACTTCCCC ATCTCAAGCC ATGCACTCTA
    CCTGCCAATG CATCACGACC TGATCGATTT GATTGGCTGA TAGCCTTTAC CCCAGATATT
    GAGCCACCAC CTACTGATAT GCGAAAACCA TCCAATGATG GCATGTTGCC AAAAGGCTCA
    ATTTCAGGCC CAAAAGTCAC CACTTTTAAA GAATTACGCA ATAGAAGCAA ACAGAGCTCC
    CCACCAGCTC CTGTTCAACC AGAACCTGAT CCAACAGTCA TCACTCCAGA CCCTGATTTC
    TTGTACAACC TCAAATGGAG AAGAGAGAAG ACAGATGGAG ATGGCTGGGA ATATACTTCC
    CAAGCACAAG CCTCTTTTCT TCAGCCTCCG CCAACTCCTG CCTCATTGTC CCTCTTTAGG
    GAAATGTGCC GCCTGAATAT ACAAGATGAA TGCAGAGCCG ATCCTAAAGT CTCCCAACAA
    ATGGGCTCAT CAGTAAGTGA AGGCAGCCTT AGGACTATTT GTGGTGATAA AGCTGCTCAC
    ACCAAAAAGA TTCAAAATGA AGATGACCTG GAGGTCAGGG GAATGGCAGA TGGAGCATGG
    ACTTTGGAGT CCAGGACAAC AGCTGTCCGC AGCTTTTCTT TTGCCGGATC TGAGCAGAAG
    AATGGGGCCT GGATGGGAGA GGATGTGAGG TCCTCAATGA GTGCTGAAGG GCTGTCTTCT
    GTATGCCTGC TAGAAAAGAA AACTCTTGTC AATTCAGTCA GTGTGGCAGT GGAGGCCATT
    TTGGCTCAGT TCAGTTCATC CAGGACTGTT GTTCAGAAGT TTCACTCAGT AGATAAGAGC
    CTTTCTGGAG ACAGCAGTGT AAATCCCTCT CTTGGTCGTC TGGTACTGCA GTGCCTTTGC
    CCAGCCCTGC GCAGCCTGCT CTGTGATGGA CTCAAGCCCC ACCAGAGTGA CCTCATTGCA
    GGCAGAAGGC CAAATTCACC CTGGGGTTTG GTTCAGGCCT CCACCAAACC AGGCCCGAGC
    ACACAGGCTT TGCACAGCCT TCAGACTAAA GTAGCAGGTC TTCATCAGCT AAGGCAGAGC
    AGGCACAGAT TCAACGCCTT CCTGTTTGGC CTTCTCAATA TCAAGCTTTT GGATTATTGG
    CTTTCCCATC TGCAGTCTTG CAGCGATGTG CTGGAAACTT TTTATCGGCC TACTTCCTTC
    ATGCGTCTGT CACTCACTTC TTGTCAGTCT CTTTTTGAGG AGCTTCTTCT CCTGCTCCAG
    CCTCTTTCTC TGCTGACCTT CAACCTGGAC CTGCTGTTTC AGCATCATCA CCTAGACCCT
    TCTTCTCCAA CTCTCACCCC AGCCAGCCAC ACGTCTGACA TATGCAGCCC ACCAAATGAA
    GACTTCAGCT TCCACCTGTC ACCCAAGGGC CTTTTCCATG GAGGTGTCCA TCGTACTGGA
    AGCATCTTAG AGCAAGACCA CAGTTTGAGT TTGGACACCA ACCCTAATTC GTTTTTTTCG
    AGTCATGTTC TTGATGTGTC AGCATATCGC AATCCAATTT CTCTGTCATC AGGCATCAAC
    AAAGAGGAAG CTGATCCGCC ATTATCATGG TTTAACGGGA ATGAGATCTC TGCACCTCTT
    AATGCTGGAA GTCTTTCCCA GCAAGCGGGC CAGGCTCTTC AGCAAGGCTG GGGGGCAGTC
    ATGCGCTTGG GGGAGCGTTT AGGGCAGAAC TTTGGTTTGG CCACCACTAC AGAGAATGCG
    AATGGTCCTT ACTCACCAGA TAATTCCAAG ACAGGCTACT CAGCAACACC AAGAAGCCCA
    GTTGAAAACA GGCAACAACC TAGAGAGGAT CTTTCCCTGT GGGATAGTGG AGCTGCTATT
    CCTTGGGGTC TTGGACGGCT CTTTGGTGCT TCTAAGAGCC CCAACAACCC ACCGACCACC
    AGGCGCCCAT CTCAGTGGCT CTCCCCTGGT GTATCCGTCC TTTCCCGGAT AGTGAATCTT
    AGCCAGGGTT CTCCACCTGA GAAAAGAGAG CTTCAGAGAA GCAATGATAA AGAGCAAGAT
    GAGAATGAAA ATATTAATGA ACCAAGAGAT CAGCCAAAGC CCCTAAGGGT GGTCAGAACT
    CTGTGTGATC ACACTGGCAC TGGGGCCGAG CTCAGCTTCA GGAAGGGAGA GGAGCTTGTT
    CTGTTGGGGG GTGTCGACCA CGACTGGATT CGATGTCGCC AAGGTCACCG AGAGGGCCTT
    GTTCCCATTG GTTATGCTTC ACTTATCATG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa21249
    Clone ID Related Accession (Same CDS sequence) XM_005157913.3 , XM_005157913.4
    Accession Version XM_005157913.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4635bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product RUN and SH3 domain-containing protein 1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005157913.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCACCCCT CCTCTCGTCC CTCTGTGCCC CCACGTACCC GCAGGTTTGA CAATAGGCGC 
    ACAGAGCCAA AACCAAGTCA AGGGGCCAAG AGGGAGGACA AAAATATGAA CACAGGATCT
    TCATCCCCTC GTCGTGGCAC TCGAGGACCA CCCGAGTCTT CCAGGTTAAA AGGCCCTGGT
    CAAGGTGCAA GGGCCCCAGT GGTTCAGTCC AAACTGACTC GGCCAGTGAG GAATGGTGCT
    CACGCAATAC CAGCAGTCTC AAAACCAAAA AGTGGGCGCG CTGTATCAAA CCTAGCCCCA
    GCTGTGGACC CAAATTGCAA TGAGCCCAGC CTTCCTTGTC TGTGCTGTGA TGGCCATTCA
    CCTCAGGATA GCAACAGCCT TTTTAATCAC AACCATAACA ACAATAACAC TGTAAACATC
    AGGCAACAAA TGAAGATACC GCCACCATCT CCTCCGAATG AGTTGCCTTT TCCAAAACAA
    GAAACTGAAG ACGATAAGTG TGAGCCAGAA GTAAAACAAC CTGATGACTT ACCTTTCCCA
    GTGGAGGAGA AAGAGGAGGA AAATGGTAAT GTGGATAAAA AAGAGGGCGA TGACCTCAAA
    AATGATGACT GCAGTGTAAA CGTGATTGGC AATGTTGATG TGAATGGCAA TGGCAATTGT
    GATGGGGATG ATGATGAAGA TGACGATGAA GATGATGATG ATACTCTTGT TCCTTCTTGC
    TGCAATTGCC CAGAATCCAT GCTGGACTTC TCTCTCACAT CTTCTACCTC ATCTTCTTCC
    ACGTCCATTA GTAGCTGCTC AGATCTGGAG TGTGACTGCC CTGATACATT TTCATTGTCA
    TCCCAGGACC AAGAAGGCCC AGAATGTTAT CCATCTTCTT GTTCCCCACT CTCCAGCTGT
    CCTTCCTTTC AGTCCAATGC CATACCATTG GATCTCAACC TTTCTGCAAG ATCACCTTGC
    TCTTCTGATG AAGGCTATCC ATCAGCACCT TGCTCTCCTT CTGACTACAC AGAGGGCAAG
    TTTTCAGAAG AAGAAAAATG TATTAAAGTG GATCTTCTCC ATTTTTTAGA CTCCATAGAA
    GAGTTGGGTA AAATGGATTT GTTCTACCGC ATTGTTAGGC TGGCACATTG GCAACTTGAT
    GGAGAACTGA TTCTCAGAGA TAGGTTGGAC CACCAGCAGA AGCTTCAAAG GGTCAACAAA
    GAAGTGAAGT TGGCTTATTT TGTGAAACTT CAAGAAGAAG GGTTCAACTT TGGCGATGAG
    GATATCACTG GAGTTTTGGA CGAAATGGGC AACATTGACA TTCCATGGAA GTTATATAAA
    AGCAACAAAT CAAGTGACTC CCAGGAATTT TCTGATGCTG GGGTGGATCT GACAGCTCCT
    TCTGACCTTG ATGAAACTGC TTCCTCTGAT TCACTTGCTC CATCACCATT AGAGCCTCCT
    CCCCGTCCCC CAAAGCCTCC AACAAGGCAT GTGAGTGTTC AGCAGGGATC ACACACTTAT
    GAGAACATCA GTGGGCATAC CTTCTCAGCC CCATCCACCA ATAATGCTAC TTCTTTCTCC
    ACAAGTGTCC CTGTTCCTTC CATGGCATCT ACATCCCCAG CCTTGAAACC TCCTGCCCTT
    CCGCCTCCAC CATCACAGCC TGTGCCCTAC TTCTCTCTTA ATTCAGACAG ACCTGCTCAC
    TCCTCCCCCA CACCACCCAT CCCTCCACCA AGAAGACGTC ATAAAGCCCG ACTAGAGGCT
    CAAAGACTTG CTGAACTTGA AAAAGAAAAG ACTCCTCTAT CCCTTCCACC TCCAACGTCA
    AGACCTCCTC CGTTGCCACC ACCACCTGCT ATGTCCTCTC CCCCAGCTAT CCCACCTCCA
    CCATCAATCC CACCGCCTCC ATCTTTTCAT GCTCTGGATG TGGAAATCCG AAAGTTGCTT
    GCACTAGCAG GACTCACCCA AGCTGAATTG CTTAAACTTA GTCCTGAATT GGGTGTTTGT
    GTAGACGTAG TTGTGGATAA TGAACAGCAT CCAATGTCTG ATGTCTCACA AATTGGAGAA
    ATCAGTGCAA ACAGAGCACA CAAGGAAGAC AGTATGGATA AGCGGTTACA TTGCAGATTG
    AGGGAAGAAA CCAGATTTGG GAAAGATATA CAGAAGGTAG AAACTCTAAG TGAAAACGAG
    ATTTCTAAAG ACGGGCAGAA AGAAGCTTAC AGGACAACAT CCTTCACAGA AATGGCAAGA
    CGACGAAAGA GAAATAGTGG AAACTGTAAC CAAAACTGCA ACTGTGGTTT TGGATCTCCC
    AACTTGTATT ACAGCACTGA TCTTAGCAAT ACAAGCATCC CAAATGTTAG CTTTGGAAGT
    TTTGATTATA CTTCAGTGAT TTCAGACACC CCACCTCCTC CACCTCCACG ACCATTACCC
    CCTTGCCCAC CAGTTATTTC CAATCCTCCT GAACTTCCCC ATCTCAAGCC ATGCACTCTA
    CCTGCCAATG CATCACGACC TGATCGATTT GATTGGCTGA TAGCCTTTAC CCCAGATATT
    GAGCCACCAC CTACTGATAT GCGAAAACCA TCCAATGATG GCATGTTGCC AAAAGGCTCA
    ATTTCAGGCC CAAAAGTCAC CACTTTTAAA GAATTACGCA ATAGAAGCAA ACAGAGCTCC
    CCACCAGCTC CTGTTCAACC AGAACCTGAT CCAACAGTCA TCACTCCAGA CCCTGATTTC
    TTGTACAACC TCAAATGGAG AAGAGAGAAG ACAGATGGAG ATGGCTGGGA ATATACTTCC
    CAAGCACAAG CCTCTTTTCT TCAGCCTCCG CCAACTCCTG CCTCATTGTC CCTCTTTAGG
    GAAATGTGCC GCCTGAATAT ACAAGATGAA TGCAGAGCCG ATCCTAAAGT CTCCCAACAA
    ATGGGCTCAT CAGTAAGTGA AGGCAGCCTT AGGACTATTT GTGGTGATAA AGCTGCTCAC
    ACCAAAAAGA TTCAAAATGA AGATGACCTG GAGGTCAGGG GAATGGCAGA TGGAGCATGG
    ACTTTGGAGT CCAGGACAAC AGCTGTCCGC AGCTTTTCTT TTGCCGGATC TGAGCAGAAG
    AATGGGGCCT GGATGGGAGA GGATGTGAGG TCCTCAATGA GTGCTGAAGG GCTGTCTTCT
    GTATGCCTGC TAGAAAAGAA AACTCTTGTC AATTCAGTCA GTGTGGCAGT GGAGGCCATT
    TTGGCTCAGT TCAGTTCATC CAGGACTGTT GTTCAGAAGA GCCTTTCTGG AGACAGCAGT
    GTAAATCCCT CTCTTGGTCG TCTGGTACTG CAGTGCCTTT GCCCAGCCCT GCGCAGCCTG
    CTCTGTGATG GACTCAAGCC CCACCAGAGT GACCTCATTG CAGGCAGAAG GCCAAATTCA
    CCCTGGGGTT TGGTTCAGGC CTCCACCAAA CCAGGCCCGA GCACACAGGC TTTGCACAGC
    CTTCAGACTA AAGTAGCAGG TCTTCATCAG CTAAGGCAGA GCAGGCACAG ATTCAACGCC
    TTCCTGTTTG GCCTTCTCAA TATCAAGCTT TTGGATTATT GGCTTTCCCA TCTGCAGTCT
    TGCAGCGATG TGCTGGAAAC TTTTTATCGG CCTACTTCCT TCATGCGTCT GTCACTCACT
    TCTTGTCAGT CTCTTTTTGA GGAGCTTCTT CTCCTGCTCC AGCCTCTTTC TCTGCTGACC
    TTCAACCTGG ACCTGCTGTT TCAGCATCAT CACCTAGACC CTTCTTCTCC AACTCTCACC
    CCAGCCAGCC ACACGTCTGA CATATGCAGC CCACCAAATG AAGACTTCAG CTTCCACCTG
    TCACCCAAGG GCCTTTTCCA TGGAGGTGTC CATCGTACTG GAAGCATCTT AGAGCAAGAC
    CACAGTTTGA GTTTGGACAC CAACCCTAAT TCGTTTTTTT CGAGTCATGT TCTTGATGTG
    TCAGCATATC GCAATCCAAT TTCTCTGTCA TCAGGCATCA ACAAAGAGGA AGCTGATCCG
    CCATTATCAT GGTTTAACGG GAATGAGATC TCTGCACCTC TTAATGCTGG AAGTCTTTCC
    CAGCAAGCGG GCCAGGCTCT TCAGCAAGGC TGGGGGGCAG TCATGCGCTT GGGGGAGCGT
    TTAGGGCAGA ACTTTGGTTT GGCCACCACT ACAGAGAATG CGAATGGTCC TTACTCACCA
    GATAATTCCA AGACAGGCTA CTCAGCAACA CCAAGAAGCC CAGTTGAAAA CAGGCAACAA
    CCTAGAGAGG ATCTTTCCCT GTGGGATAGT GGAGCTGCTA TTCCTTGGGG TCTTGGACGG
    CTCTTTGGTG CTTCTAAGAG CCCCAACAAC CCACCGACCA CCAGGCGCCC ATCTCAGTGG
    CTCTCCCCTG GTGTATCCGT CCTTTCCCGG ATAGTGAATC TTAGCCAGGG TTCTCCACCT
    GAGAAAAGAG AGCTTCAGAG AAGCAATGAT AAAGAGCAAG ATGAGAATGA AAATATTAAT
    GAACCAAGAG ATCAGCCAAA GCCCCTAAGG GTGGTCAGAA CTCTGTGTGA TCACACTGGC
    ACTGGGGCCG AGCTCAGCTT CAGGAAGGGA GAGGAGCTTG TTCTGTTGGG GGGTGTCGAC
    CACGACTGGA TTCGATGTCG CCAAGGTCAC CGAGAGGGCC TTGTTCCCAT TGGTTATGCT
    TCACTTATCA TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005157970.1
    CDS294..4928
    Translation

    Target ORF information:

    RefSeq Version XM_005157913.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio RUN and SH3 domain containing 1 (rusc1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005157913.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCACCCCT CCTCTCGTCC CTCTGTGCCC CCACGTACCC GCAGGTTTGA CAATAGGCGC 
    ACAGAGCCAA AACCAAGTCA AGGGGCCAAG AGGGAGGACA AAAATATGAA CACAGGATCT
    TCATCCCCTC GTCGTGGCAC TCGAGGACCA CCCGAGTCTT CCAGGTTAAA AGGCCCTGGT
    CAAGGTGCAA GGGCCCCAGT GGTTCAGTCC AAACTGACTC GGCCAGTGAG GAATGGTGCT
    CACGCAATAC CAGCAGTCTC AAAACCAAAA AGTGGGCGCG CTGTATCAAA CCTAGCCCCA
    GCTGTGGACC CAAATTGCAA TGAGCCCAGC CTTCCTTGTC TGTGCTGTGA TGGCCATTCA
    CCTCAGGATA GCAACAGCCT TTTTAATCAC AACCATAACA ACAATAACAC TGTAAACATC
    AGGCAACAAA TGAAGATACC GCCACCATCT CCTCCGAATG AGTTGCCTTT TCCAAAACAA
    GAAACTGAAG ACGATAAGTG TGAGCCAGAA GTAAAACAAC CTGATGACTT ACCTTTCCCA
    GTGGAGGAGA AAGAGGAGGA AAATGGTAAT GTGGATAAAA AAGAGGGCGA TGACCTCAAA
    AATGATGACT GCAGTGTAAA CGTGATTGGC AATGTTGATG TGAATGGCAA TGGCAATTGT
    GATGGGGATG ATGATGAAGA TGACGATGAA GATGATGATG ATACTCTTGT TCCTTCTTGC
    TGCAATTGCC CAGAATCCAT GCTGGACTTC TCTCTCACAT CTTCTACCTC ATCTTCTTCC
    ACGTCCATTA GTAGCTGCTC AGATCTGGAG TGTGACTGCC CTGATACATT TTCATTGTCA
    TCCCAGGACC AAGAAGGCCC AGAATGTTAT CCATCTTCTT GTTCCCCACT CTCCAGCTGT
    CCTTCCTTTC AGTCCAATGC CATACCATTG GATCTCAACC TTTCTGCAAG ATCACCTTGC
    TCTTCTGATG AAGGCTATCC ATCAGCACCT TGCTCTCCTT CTGACTACAC AGAGGGCAAG
    TTTTCAGAAG AAGAAAAATG TATTAAAGTG GATCTTCTCC ATTTTTTAGA CTCCATAGAA
    GAGTTGGGTA AAATGGATTT GTTCTACCGC ATTGTTAGGC TGGCACATTG GCAACTTGAT
    GGAGAACTGA TTCTCAGAGA TAGGTTGGAC CACCAGCAGA AGCTTCAAAG GGTCAACAAA
    GAAGTGAAGT TGGCTTATTT TGTGAAACTT CAAGAAGAAG GGTTCAACTT TGGCGATGAG
    GATATCACTG GAGTTTTGGA CGAAATGGGC AACATTGACA TTCCATGGAA GTTATATAAA
    AGCAACAAAT CAAGTGACTC CCAGGAATTT TCTGATGCTG GGGTGGATCT GACAGCTCCT
    TCTGACCTTG ATGAAACTGC TTCCTCTGAT TCACTTGCTC CATCACCATT AGAGCCTCCT
    CCCCGTCCCC CAAAGCCTCC AACAAGGCAT GTGAGTGTTC AGCAGGGATC ACACACTTAT
    GAGAACATCA GTGGGCATAC CTTCTCAGCC CCATCCACCA ATAATGCTAC TTCTTTCTCC
    ACAAGTGTCC CTGTTCCTTC CATGGCATCT ACATCCCCAG CCTTGAAACC TCCTGCCCTT
    CCGCCTCCAC CATCACAGCC TGTGCCCTAC TTCTCTCTTA ATTCAGACAG ACCTGCTCAC
    TCCTCCCCCA CACCACCCAT CCCTCCACCA AGAAGACGTC ATAAAGCCCG ACTAGAGGCT
    CAAAGACTTG CTGAACTTGA AAAAGAAAAG ACTCCTCTAT CCCTTCCACC TCCAACGTCA
    AGACCTCCTC CGTTGCCACC ACCACCTGCT ATGTCCTCTC CCCCAGCTAT CCCACCTCCA
    CCATCAATCC CACCGCCTCC ATCTTTTCAT GCTCTGGATG TGGAAATCCG AAAGTTGCTT
    GCACTAGCAG GACTCACCCA AGCTGAATTG CTTAAACTTA GTCCTGAATT GGGTGTTTGT
    GTAGACGTAG TTGTGGATAA TGAACAGCAT CCAATGTCTG ATGTCTCACA AATTGGAGAA
    ATCAGTGCAA ACAGAGCACA CAAGGAAGAC AGTATGGATA AGCGGTTACA TTGCAGATTG
    AGGGAAGAAA CCAGATTTGG GAAAGATATA CAGAAGGTAG AAACTCTAAG TGAAAACGAG
    ATTTCTAAAG ACGGGCAGAA AGAAGCTTAC AGGACAACAT CCTTCACAGA AATGGCAAGA
    CGACGAAAGA GAAATAGTGG AAACTGTAAC CAAAACTGCA ACTGTGGTTT TGGATCTCCC
    AACTTGTATT ACAGCACTGA TCTTAGCAAT ACAAGCATCC CAAATGTTAG CTTTGGAAGT
    TTTGATTATA CTTCAGTGAT TTCAGACACC CCACCTCCTC CACCTCCACG ACCATTACCC
    CCTTGCCCAC CAGTTATTTC CAATCCTCCT GAACTTCCCC ATCTCAAGCC ATGCACTCTA
    CCTGCCAATG CATCACGACC TGATCGATTT GATTGGCTGA TAGCCTTTAC CCCAGATATT
    GAGCCACCAC CTACTGATAT GCGAAAACCA TCCAATGATG GCATGTTGCC AAAAGGCTCA
    ATTTCAGGCC CAAAAGTCAC CACTTTTAAA GAATTACGCA ATAGAAGCAA ACAGAGCTCC
    CCACCAGCTC CTGTTCAACC AGAACCTGAT CCAACAGTCA TCACTCCAGA CCCTGATTTC
    TTGTACAACC TCAAATGGAG AAGAGAGAAG ACAGATGGAG ATGGCTGGGA ATATACTTCC
    CAAGCACAAG CCTCTTTTCT TCAGCCTCCG CCAACTCCTG CCTCATTGTC CCTCTTTAGG
    GAAATGTGCC GCCTGAATAT ACAAGATGAA TGCAGAGCCG ATCCTAAAGT CTCCCAACAA
    ATGGGCTCAT CAGTAAGTGA AGGCAGCCTT AGGACTATTT GTGGTGATAA AGCTGCTCAC
    ACCAAAAAGA TTCAAAATGA AGATGACCTG GAGGTCAGGG GAATGGCAGA TGGAGCATGG
    ACTTTGGAGT CCAGGACAAC AGCTGTCCGC AGCTTTTCTT TTGCCGGATC TGAGCAGAAG
    AATGGGGCCT GGATGGGAGA GGATGTGAGG TCCTCAATGA GTGCTGAAGG GCTGTCTTCT
    GTATGCCTGC TAGAAAAGAA AACTCTTGTC AATTCAGTCA GTGTGGCAGT GGAGGCCATT
    TTGGCTCAGT TCAGTTCATC CAGGACTGTT GTTCAGAAGA GCCTTTCTGG AGACAGCAGT
    GTAAATCCCT CTCTTGGTCG TCTGGTACTG CAGTGCCTTT GCCCAGCCCT GCGCAGCCTG
    CTCTGTGATG GACTCAAGCC CCACCAGAGT GACCTCATTG CAGGCAGAAG GCCAAATTCA
    CCCTGGGGTT TGGTTCAGGC CTCCACCAAA CCAGGCCCGA GCACACAGGC TTTGCACAGC
    CTTCAGACTA AAGTAGCAGG TCTTCATCAG CTAAGGCAGA GCAGGCACAG ATTCAACGCC
    TTCCTGTTTG GCCTTCTCAA TATCAAGCTT TTGGATTATT GGCTTTCCCA TCTGCAGTCT
    TGCAGCGATG TGCTGGAAAC TTTTTATCGG CCTACTTCCT TCATGCGTCT GTCACTCACT
    TCTTGTCAGT CTCTTTTTGA GGAGCTTCTT CTCCTGCTCC AGCCTCTTTC TCTGCTGACC
    TTCAACCTGG ACCTGCTGTT TCAGCATCAT CACCTAGACC CTTCTTCTCC AACTCTCACC
    CCAGCCAGCC ACACGTCTGA CATATGCAGC CCACCAAATG AAGACTTCAG CTTCCACCTG
    TCACCCAAGG GCCTTTTCCA TGGAGGTGTC CATCGTACTG GAAGCATCTT AGAGCAAGAC
    CACAGTTTGA GTTTGGACAC CAACCCTAAT TCGTTTTTTT CGAGTCATGT TCTTGATGTG
    TCAGCATATC GCAATCCAAT TTCTCTGTCA TCAGGCATCA ACAAAGAGGA AGCTGATCCG
    CCATTATCAT GGTTTAACGG GAATGAGATC TCTGCACCTC TTAATGCTGG AAGTCTTTCC
    CAGCAAGCGG GCCAGGCTCT TCAGCAAGGC TGGGGGGCAG TCATGCGCTT GGGGGAGCGT
    TTAGGGCAGA ACTTTGGTTT GGCCACCACT ACAGAGAATG CGAATGGTCC TTACTCACCA
    GATAATTCCA AGACAGGCTA CTCAGCAACA CCAAGAAGCC CAGTTGAAAA CAGGCAACAA
    CCTAGAGAGG ATCTTTCCCT GTGGGATAGT GGAGCTGCTA TTCCTTGGGG TCTTGGACGG
    CTCTTTGGTG CTTCTAAGAG CCCCAACAAC CCACCGACCA CCAGGCGCCC ATCTCAGTGG
    CTCTCCCCTG GTGTATCCGT CCTTTCCCGG ATAGTGAATC TTAGCCAGGG TTCTCCACCT
    GAGAAAAGAG AGCTTCAGAG AAGCAATGAT AAAGAGCAAG ATGAGAATGA AAATATTAAT
    GAACCAAGAG ATCAGCCAAA GCCCCTAAGG GTGGTCAGAA CTCTGTGTGA TCACACTGGC
    ACTGGGGCCG AGCTCAGCTT CAGGAAGGGA GAGGAGCTTG TTCTGTTGGG GGGTGTCGAC
    CACGACTGGA TTCGATGTCG CCAAGGTCAC CGAGAGGGCC TTGTTCCCAT TGGTTATGCT
    TCACTTATCA TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa21249
    Clone ID Related Accession (Same CDS sequence) XM_005157913.3 , XM_005157913.4
    Accession Version XM_005157913.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4635bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product RUN and SH3 domain-containing protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005157913.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCACCCCT CCTCTCGTCC CTCTGTGCCC CCACGTACCC GCAGGTTTGA CAATAGGCGC 
    ACAGAGCCAA AACCAAGTCA AGGGGCCAAG AGGGAGGACA AAAATATGAA CACAGGATCT
    TCATCCCCTC GTCGTGGCAC TCGAGGACCA CCCGAGTCTT CCAGGTTAAA AGGCCCTGGT
    CAAGGTGCAA GGGCCCCAGT GGTTCAGTCC AAACTGACTC GGCCAGTGAG GAATGGTGCT
    CACGCAATAC CAGCAGTCTC AAAACCAAAA AGTGGGCGCG CTGTATCAAA CCTAGCCCCA
    GCTGTGGACC CAAATTGCAA TGAGCCCAGC CTTCCTTGTC TGTGCTGTGA TGGCCATTCA
    CCTCAGGATA GCAACAGCCT TTTTAATCAC AACCATAACA ACAATAACAC TGTAAACATC
    AGGCAACAAA TGAAGATACC GCCACCATCT CCTCCGAATG AGTTGCCTTT TCCAAAACAA
    GAAACTGAAG ACGATAAGTG TGAGCCAGAA GTAAAACAAC CTGATGACTT ACCTTTCCCA
    GTGGAGGAGA AAGAGGAGGA AAATGGTAAT GTGGATAAAA AAGAGGGCGA TGACCTCAAA
    AATGATGACT GCAGTGTAAA CGTGATTGGC AATGTTGATG TGAATGGCAA TGGCAATTGT
    GATGGGGATG ATGATGAAGA TGACGATGAA GATGATGATG ATACTCTTGT TCCTTCTTGC
    TGCAATTGCC CAGAATCCAT GCTGGACTTC TCTCTCACAT CTTCTACCTC ATCTTCTTCC
    ACGTCCATTA GTAGCTGCTC AGATCTGGAG TGTGACTGCC CTGATACATT TTCATTGTCA
    TCCCAGGACC AAGAAGGCCC AGAATGTTAT CCATCTTCTT GTTCCCCACT CTCCAGCTGT
    CCTTCCTTTC AGTCCAATGC CATACCATTG GATCTCAACC TTTCTGCAAG ATCACCTTGC
    TCTTCTGATG AAGGCTATCC ATCAGCACCT TGCTCTCCTT CTGACTACAC AGAGGGCAAG
    TTTTCAGAAG AAGAAAAATG TATTAAAGTG GATCTTCTCC ATTTTTTAGA CTCCATAGAA
    GAGTTGGGTA AAATGGATTT GTTCTACCGC ATTGTTAGGC TGGCACATTG GCAACTTGAT
    GGAGAACTGA TTCTCAGAGA TAGGTTGGAC CACCAGCAGA AGCTTCAAAG GGTCAACAAA
    GAAGTGAAGT TGGCTTATTT TGTGAAACTT CAAGAAGAAG GGTTCAACTT TGGCGATGAG
    GATATCACTG GAGTTTTGGA CGAAATGGGC AACATTGACA TTCCATGGAA GTTATATAAA
    AGCAACAAAT CAAGTGACTC CCAGGAATTT TCTGATGCTG GGGTGGATCT GACAGCTCCT
    TCTGACCTTG ATGAAACTGC TTCCTCTGAT TCACTTGCTC CATCACCATT AGAGCCTCCT
    CCCCGTCCCC CAAAGCCTCC AACAAGGCAT GTGAGTGTTC AGCAGGGATC ACACACTTAT
    GAGAACATCA GTGGGCATAC CTTCTCAGCC CCATCCACCA ATAATGCTAC TTCTTTCTCC
    ACAAGTGTCC CTGTTCCTTC CATGGCATCT ACATCCCCAG CCTTGAAACC TCCTGCCCTT
    CCGCCTCCAC CATCACAGCC TGTGCCCTAC TTCTCTCTTA ATTCAGACAG ACCTGCTCAC
    TCCTCCCCCA CACCACCCAT CCCTCCACCA AGAAGACGTC ATAAAGCCCG ACTAGAGGCT
    CAAAGACTTG CTGAACTTGA AAAAGAAAAG ACTCCTCTAT CCCTTCCACC TCCAACGTCA
    AGACCTCCTC CGTTGCCACC ACCACCTGCT ATGTCCTCTC CCCCAGCTAT CCCACCTCCA
    CCATCAATCC CACCGCCTCC ATCTTTTCAT GCTCTGGATG TGGAAATCCG AAAGTTGCTT
    GCACTAGCAG GACTCACCCA AGCTGAATTG CTTAAACTTA GTCCTGAATT GGGTGTTTGT
    GTAGACGTAG TTGTGGATAA TGAACAGCAT CCAATGTCTG ATGTCTCACA AATTGGAGAA
    ATCAGTGCAA ACAGAGCACA CAAGGAAGAC AGTATGGATA AGCGGTTACA TTGCAGATTG
    AGGGAAGAAA CCAGATTTGG GAAAGATATA CAGAAGGTAG AAACTCTAAG TGAAAACGAG
    ATTTCTAAAG ACGGGCAGAA AGAAGCTTAC AGGACAACAT CCTTCACAGA AATGGCAAGA
    CGACGAAAGA GAAATAGTGG AAACTGTAAC CAAAACTGCA ACTGTGGTTT TGGATCTCCC
    AACTTGTATT ACAGCACTGA TCTTAGCAAT ACAAGCATCC CAAATGTTAG CTTTGGAAGT
    TTTGATTATA CTTCAGTGAT TTCAGACACC CCACCTCCTC CACCTCCACG ACCATTACCC
    CCTTGCCCAC CAGTTATTTC CAATCCTCCT GAACTTCCCC ATCTCAAGCC ATGCACTCTA
    CCTGCCAATG CATCACGACC TGATCGATTT GATTGGCTGA TAGCCTTTAC CCCAGATATT
    GAGCCACCAC CTACTGATAT GCGAAAACCA TCCAATGATG GCATGTTGCC AAAAGGCTCA
    ATTTCAGGCC CAAAAGTCAC CACTTTTAAA GAATTACGCA ATAGAAGCAA ACAGAGCTCC
    CCACCAGCTC CTGTTCAACC AGAACCTGAT CCAACAGTCA TCACTCCAGA CCCTGATTTC
    TTGTACAACC TCAAATGGAG AAGAGAGAAG ACAGATGGAG ATGGCTGGGA ATATACTTCC
    CAAGCACAAG CCTCTTTTCT TCAGCCTCCG CCAACTCCTG CCTCATTGTC CCTCTTTAGG
    GAAATGTGCC GCCTGAATAT ACAAGATGAA TGCAGAGCCG ATCCTAAAGT CTCCCAACAA
    ATGGGCTCAT CAGTAAGTGA AGGCAGCCTT AGGACTATTT GTGGTGATAA AGCTGCTCAC
    ACCAAAAAGA TTCAAAATGA AGATGACCTG GAGGTCAGGG GAATGGCAGA TGGAGCATGG
    ACTTTGGAGT CCAGGACAAC AGCTGTCCGC AGCTTTTCTT TTGCCGGATC TGAGCAGAAG
    AATGGGGCCT GGATGGGAGA GGATGTGAGG TCCTCAATGA GTGCTGAAGG GCTGTCTTCT
    GTATGCCTGC TAGAAAAGAA AACTCTTGTC AATTCAGTCA GTGTGGCAGT GGAGGCCATT
    TTGGCTCAGT TCAGTTCATC CAGGACTGTT GTTCAGAAGA GCCTTTCTGG AGACAGCAGT
    GTAAATCCCT CTCTTGGTCG TCTGGTACTG CAGTGCCTTT GCCCAGCCCT GCGCAGCCTG
    CTCTGTGATG GACTCAAGCC CCACCAGAGT GACCTCATTG CAGGCAGAAG GCCAAATTCA
    CCCTGGGGTT TGGTTCAGGC CTCCACCAAA CCAGGCCCGA GCACACAGGC TTTGCACAGC
    CTTCAGACTA AAGTAGCAGG TCTTCATCAG CTAAGGCAGA GCAGGCACAG ATTCAACGCC
    TTCCTGTTTG GCCTTCTCAA TATCAAGCTT TTGGATTATT GGCTTTCCCA TCTGCAGTCT
    TGCAGCGATG TGCTGGAAAC TTTTTATCGG CCTACTTCCT TCATGCGTCT GTCACTCACT
    TCTTGTCAGT CTCTTTTTGA GGAGCTTCTT CTCCTGCTCC AGCCTCTTTC TCTGCTGACC
    TTCAACCTGG ACCTGCTGTT TCAGCATCAT CACCTAGACC CTTCTTCTCC AACTCTCACC
    CCAGCCAGCC ACACGTCTGA CATATGCAGC CCACCAAATG AAGACTTCAG CTTCCACCTG
    TCACCCAAGG GCCTTTTCCA TGGAGGTGTC CATCGTACTG GAAGCATCTT AGAGCAAGAC
    CACAGTTTGA GTTTGGACAC CAACCCTAAT TCGTTTTTTT CGAGTCATGT TCTTGATGTG
    TCAGCATATC GCAATCCAAT TTCTCTGTCA TCAGGCATCA ACAAAGAGGA AGCTGATCCG
    CCATTATCAT GGTTTAACGG GAATGAGATC TCTGCACCTC TTAATGCTGG AAGTCTTTCC
    CAGCAAGCGG GCCAGGCTCT TCAGCAAGGC TGGGGGGCAG TCATGCGCTT GGGGGAGCGT
    TTAGGGCAGA ACTTTGGTTT GGCCACCACT ACAGAGAATG CGAATGGTCC TTACTCACCA
    GATAATTCCA AGACAGGCTA CTCAGCAACA CCAAGAAGCC CAGTTGAAAA CAGGCAACAA
    CCTAGAGAGG ATCTTTCCCT GTGGGATAGT GGAGCTGCTA TTCCTTGGGG TCTTGGACGG
    CTCTTTGGTG CTTCTAAGAG CCCCAACAAC CCACCGACCA CCAGGCGCCC ATCTCAGTGG
    CTCTCCCCTG GTGTATCCGT CCTTTCCCGG ATAGTGAATC TTAGCCAGGG TTCTCCACCT
    GAGAAAAGAG AGCTTCAGAG AAGCAATGAT AAAGAGCAAG ATGAGAATGA AAATATTAAT
    GAACCAAGAG ATCAGCCAAA GCCCCTAAGG GTGGTCAGAA CTCTGTGTGA TCACACTGGC
    ACTGGGGCCG AGCTCAGCTT CAGGAAGGGA GAGGAGCTTG TTCTGTTGGG GGGTGTCGAC
    CACGACTGGA TTCGATGTCG CCAAGGTCAC CGAGAGGGCC TTGTTCCCAT TGGTTATGCT
    TCACTTATCA TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005157970.1
    CDS279..4913
    Translation

    Target ORF information:

    RefSeq Version XM_005157913.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio RUN and SH3 domain containing 1 (rusc1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005157913.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCACCCCT CCTCTCGTCC CTCTGTGCCC CCACGTACCC GCAGGTTTGA CAATAGGCGC 
    ACAGAGCCAA AACCAAGTCA AGGGGCCAAG AGGGAGGACA AAAATATGAA CACAGGATCT
    TCATCCCCTC GTCGTGGCAC TCGAGGACCA CCCGAGTCTT CCAGGTTAAA AGGCCCTGGT
    CAAGGTGCAA GGGCCCCAGT GGTTCAGTCC AAACTGACTC GGCCAGTGAG GAATGGTGCT
    CACGCAATAC CAGCAGTCTC AAAACCAAAA AGTGGGCGCG CTGTATCAAA CCTAGCCCCA
    GCTGTGGACC CAAATTGCAA TGAGCCCAGC CTTCCTTGTC TGTGCTGTGA TGGCCATTCA
    CCTCAGGATA GCAACAGCCT TTTTAATCAC AACCATAACA ACAATAACAC TGTAAACATC
    AGGCAACAAA TGAAGATACC GCCACCATCT CCTCCGAATG AGTTGCCTTT TCCAAAACAA
    GAAACTGAAG ACGATAAGTG TGAGCCAGAA GTAAAACAAC CTGATGACTT ACCTTTCCCA
    GTGGAGGAGA AAGAGGAGGA AAATGGTAAT GTGGATAAAA AAGAGGGCGA TGACCTCAAA
    AATGATGACT GCAGTGTAAA CGTGATTGGC AATGTTGATG TGAATGGCAA TGGCAATTGT
    GATGGGGATG ATGATGAAGA TGACGATGAA GATGATGATG ATACTCTTGT TCCTTCTTGC
    TGCAATTGCC CAGAATCCAT GCTGGACTTC TCTCTCACAT CTTCTACCTC ATCTTCTTCC
    ACGTCCATTA GTAGCTGCTC AGATCTGGAG TGTGACTGCC CTGATACATT TTCATTGTCA
    TCCCAGGACC AAGAAGGCCC AGAATGTTAT CCATCTTCTT GTTCCCCACT CTCCAGCTGT
    CCTTCCTTTC AGTCCAATGC CATACCATTG GATCTCAACC TTTCTGCAAG ATCACCTTGC
    TCTTCTGATG AAGGCTATCC ATCAGCACCT TGCTCTCCTT CTGACTACAC AGAGGGCAAG
    TTTTCAGAAG AAGAAAAATG TATTAAAGTG GATCTTCTCC ATTTTTTAGA CTCCATAGAA
    GAGTTGGGTA AAATGGATTT GTTCTACCGC ATTGTTAGGC TGGCACATTG GCAACTTGAT
    GGAGAACTGA TTCTCAGAGA TAGGTTGGAC CACCAGCAGA AGCTTCAAAG GGTCAACAAA
    GAAGTGAAGT TGGCTTATTT TGTGAAACTT CAAGAAGAAG GGTTCAACTT TGGCGATGAG
    GATATCACTG GAGTTTTGGA CGAAATGGGC AACATTGACA TTCCATGGAA GTTATATAAA
    AGCAACAAAT CAAGTGACTC CCAGGAATTT TCTGATGCTG GGGTGGATCT GACAGCTCCT
    TCTGACCTTG ATGAAACTGC TTCCTCTGAT TCACTTGCTC CATCACCATT AGAGCCTCCT
    CCCCGTCCCC CAAAGCCTCC AACAAGGCAT GTGAGTGTTC AGCAGGGATC ACACACTTAT
    GAGAACATCA GTGGGCATAC CTTCTCAGCC CCATCCACCA ATAATGCTAC TTCTTTCTCC
    ACAAGTGTCC CTGTTCCTTC CATGGCATCT ACATCCCCAG CCTTGAAACC TCCTGCCCTT
    CCGCCTCCAC CATCACAGCC TGTGCCCTAC TTCTCTCTTA ATTCAGACAG ACCTGCTCAC
    TCCTCCCCCA CACCACCCAT CCCTCCACCA AGAAGACGTC ATAAAGCCCG ACTAGAGGCT
    CAAAGACTTG CTGAACTTGA AAAAGAAAAG ACTCCTCTAT CCCTTCCACC TCCAACGTCA
    AGACCTCCTC CGTTGCCACC ACCACCTGCT ATGTCCTCTC CCCCAGCTAT CCCACCTCCA
    CCATCAATCC CACCGCCTCC ATCTTTTCAT GCTCTGGATG TGGAAATCCG AAAGTTGCTT
    GCACTAGCAG GACTCACCCA AGCTGAATTG CTTAAACTTA GTCCTGAATT GGGTGTTTGT
    GTAGACGTAG TTGTGGATAA TGAACAGCAT CCAATGTCTG ATGTCTCACA AATTGGAGAA
    ATCAGTGCAA ACAGAGCACA CAAGGAAGAC AGTATGGATA AGCGGTTACA TTGCAGATTG
    AGGGAAGAAA CCAGATTTGG GAAAGATATA CAGAAGGTAG AAACTCTAAG TGAAAACGAG
    ATTTCTAAAG ACGGGCAGAA AGAAGCTTAC AGGACAACAT CCTTCACAGA AATGGCAAGA
    CGACGAAAGA GAAATAGTGG AAACTGTAAC CAAAACTGCA ACTGTGGTTT TGGATCTCCC
    AACTTGTATT ACAGCACTGA TCTTAGCAAT ACAAGCATCC CAAATGTTAG CTTTGGAAGT
    TTTGATTATA CTTCAGTGAT TTCAGACACC CCACCTCCTC CACCTCCACG ACCATTACCC
    CCTTGCCCAC CAGTTATTTC CAATCCTCCT GAACTTCCCC ATCTCAAGCC ATGCACTCTA
    CCTGCCAATG CATCACGACC TGATCGATTT GATTGGCTGA TAGCCTTTAC CCCAGATATT
    GAGCCACCAC CTACTGATAT GCGAAAACCA TCCAATGATG GCATGTTGCC AAAAGGCTCA
    ATTTCAGGCC CAAAAGTCAC CACTTTTAAA GAATTACGCA ATAGAAGCAA ACAGAGCTCC
    CCACCAGCTC CTGTTCAACC AGAACCTGAT CCAACAGTCA TCACTCCAGA CCCTGATTTC
    TTGTACAACC TCAAATGGAG AAGAGAGAAG ACAGATGGAG ATGGCTGGGA ATATACTTCC
    CAAGCACAAG CCTCTTTTCT TCAGCCTCCG CCAACTCCTG CCTCATTGTC CCTCTTTAGG
    GAAATGTGCC GCCTGAATAT ACAAGATGAA TGCAGAGCCG ATCCTAAAGT CTCCCAACAA
    ATGGGCTCAT CAGTAAGTGA AGGCAGCCTT AGGACTATTT GTGGTGATAA AGCTGCTCAC
    ACCAAAAAGA TTCAAAATGA AGATGACCTG GAGGTCAGGG GAATGGCAGA TGGAGCATGG
    ACTTTGGAGT CCAGGACAAC AGCTGTCCGC AGCTTTTCTT TTGCCGGATC TGAGCAGAAG
    AATGGGGCCT GGATGGGAGA GGATGTGAGG TCCTCAATGA GTGCTGAAGG GCTGTCTTCT
    GTATGCCTGC TAGAAAAGAA AACTCTTGTC AATTCAGTCA GTGTGGCAGT GGAGGCCATT
    TTGGCTCAGT TCAGTTCATC CAGGACTGTT GTTCAGAAGA GCCTTTCTGG AGACAGCAGT
    GTAAATCCCT CTCTTGGTCG TCTGGTACTG CAGTGCCTTT GCCCAGCCCT GCGCAGCCTG
    CTCTGTGATG GACTCAAGCC CCACCAGAGT GACCTCATTG CAGGCAGAAG GCCAAATTCA
    CCCTGGGGTT TGGTTCAGGC CTCCACCAAA CCAGGCCCGA GCACACAGGC TTTGCACAGC
    CTTCAGACTA AAGTAGCAGG TCTTCATCAG CTAAGGCAGA GCAGGCACAG ATTCAACGCC
    TTCCTGTTTG GCCTTCTCAA TATCAAGCTT TTGGATTATT GGCTTTCCCA TCTGCAGTCT
    TGCAGCGATG TGCTGGAAAC TTTTTATCGG CCTACTTCCT TCATGCGTCT GTCACTCACT
    TCTTGTCAGT CTCTTTTTGA GGAGCTTCTT CTCCTGCTCC AGCCTCTTTC TCTGCTGACC
    TTCAACCTGG ACCTGCTGTT TCAGCATCAT CACCTAGACC CTTCTTCTCC AACTCTCACC
    CCAGCCAGCC ACACGTCTGA CATATGCAGC CCACCAAATG AAGACTTCAG CTTCCACCTG
    TCACCCAAGG GCCTTTTCCA TGGAGGTGTC CATCGTACTG GAAGCATCTT AGAGCAAGAC
    CACAGTTTGA GTTTGGACAC CAACCCTAAT TCGTTTTTTT CGAGTCATGT TCTTGATGTG
    TCAGCATATC GCAATCCAAT TTCTCTGTCA TCAGGCATCA ACAAAGAGGA AGCTGATCCG
    CCATTATCAT GGTTTAACGG GAATGAGATC TCTGCACCTC TTAATGCTGG AAGTCTTTCC
    CAGCAAGCGG GCCAGGCTCT TCAGCAAGGC TGGGGGGCAG TCATGCGCTT GGGGGAGCGT
    TTAGGGCAGA ACTTTGGTTT GGCCACCACT ACAGAGAATG CGAATGGTCC TTACTCACCA
    GATAATTCCA AGACAGGCTA CTCAGCAACA CCAAGAAGCC CAGTTGAAAA CAGGCAACAA
    CCTAGAGAGG ATCTTTCCCT GTGGGATAGT GGAGCTGCTA TTCCTTGGGG TCTTGGACGG
    CTCTTTGGTG CTTCTAAGAG CCCCAACAAC CCACCGACCA CCAGGCGCCC ATCTCAGTGG
    CTCTCCCCTG GTGTATCCGT CCTTTCCCGG ATAGTGAATC TTAGCCAGGG TTCTCCACCT
    GAGAAAAGAG AGCTTCAGAG AAGCAATGAT AAAGAGCAAG ATGAGAATGA AAATATTAAT
    GAACCAAGAG ATCAGCCAAA GCCCCTAAGG GTGGTCAGAA CTCTGTGTGA TCACACTGGC
    ACTGGGGCCG AGCTCAGCTT CAGGAAGGGA GAGGAGCTTG TTCTGTTGGG GGGTGTCGAC
    CACGACTGGA TTCGATGTCG CCAAGGTCAC CGAGAGGGCC TTGTTCCCAT TGGTTATGCT
    TCACTTATCA TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.