ssh2b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ssh2b
Entrez Gene ID	557912
Full Name	slingshot protein phosphatase 2b
Synonyms	zgc:175142
General protein information	Preferred Names slingshot protein phosphatase 2b Names protein phosphatase Slingshot homolog 2 slingshot homolog 2b
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001135103.1	NP_001128575.1	protein phosphatase Slingshot homolog 2
XM_009295652.3	XP_009293927.1	protein phosphatase Slingshot homolog 2 isoform X2
XM_009295650.2	XP_009293925.1	protein phosphatase Slingshot homolog 2 isoform X1
XM_009295653.2	XP_009293928.1	protein phosphatase Slingshot homolog 2 isoform X3
XM_009295653.3	XP_009293928.1	protein phosphatase Slingshot homolog 2 isoform X3
XM_009295652.2	XP_009293927.1	protein phosphatase Slingshot homolog 2 isoform X2
XM_009295650.3	XP_009293925.1	protein phosphatase Slingshot homolog 2 isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Evolution of the tbx6/16 subfamily genes in vertebrates: insights from zebrafish.
Ahn D, You KH, Kim CH
Molecular biology and evolution29(12)3959-83(2012 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ssh2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ssh2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa42698	XM_009295652.3 Latest version!	Danio rerio slingshot protein phosphatase 2b (ssh2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa42699	XM_009295653.3 Latest version!	Danio rerio slingshot protein phosphatase 2b (ssh2b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa03810	NM_001135103.1 Latest version!	Danio rerio slingshot protein phosphatase 2b (ssh2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa26174	XM_009295650.3 Latest version!	Danio rerio slingshot protein phosphatase 2b (ssh2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
ODa42699	XM_009295653.2	Danio rerio slingshot protein phosphatase 2b (ssh2b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa42698	XM_009295652.2	Danio rerio slingshot protein phosphatase 2b (ssh2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa26174	XM_009295650.2	Danio rerio slingshot protein phosphatase 2b (ssh2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ssh2b ( XM_009295652.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293927.1 Danio rerio slingshot protein phosphatase 2b (ssh2b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa42698
Clone ID Related Accession (Same CDS sequence)	XM_009295652.3 , XM_009295652.2
Accession Version	XM_009295652.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein phosphatase Slingshot homolog 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009295652.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGTTT CCTCACCGCT GTCTGATGTA AAGACGTTAA GTGGTCTGGA GACTGATTTA 
AACACATCCA CAGAATGTCT GTTGGCTCAC GATGGGATAC AAATACTGCT GCAAAGTGGC 
TTCATGCACC AGGACAACAT CAAACAGTTC ATCAGTGAGA GCTTCCTAAC AGTCAAAGGT 
GCCGCCCTTT TCCTTCCACG TGGGAATGGC TCCTCTGCTG CCTCGTCCTG CTCCTCCAGA 
ATCACAGCAC AGCGCAGCAA ACATGCAGGG GACCTTCAGC AGCACCTGCA GACCATGTTC 
ACTTTACTGC GGCCCGAGGA TAACATCCGC TTGGCTGTGC GGTTGGAGAG CACCTTTCCC 
CAGTGCACCC GCTACATGGT GGTGGTCTCC ACTAATGGCA GGCAGGACAC AGAAGAGAGT 
GTGGTGCTGG GAATGGACTT CAGCCCCTCC GACAGTTCCT GTTCCATAGG CATGGTGCTT 
CCCTTGTGGA GTGACACACG GATCCATCTG GATGGTGACG GGGGCTTCAG TGTGTCTACA 
GATAACAAAA TACACATATT CAAGCCAGTG TCAGTTCAAG CCATGTGGTC TGCCTTGCAG 
TCGCTTCATA AGGCCTGTGA AGTGGCCCGC TGTCATAACT ACTTCCCGGG CAGCCTCTTC 
CTGACCTGGG TGAGTTACTA CCAGAGCCGG GTGTCCTCAG ATCAGGCCCG CATCAACGAG 
TGGAACGCCA TGCAGGACGT GCAGTCCCAC CGTGCAGACT CACCTGTGCT CTTCTCTGAT 
GTGCCAACTG AGCGGGAAAG AACAGAGAGA TTCATCAAGA CTCGATTAAG AGAGATCATG 
ATGCTAAAGG ATCTTGAAAA TGTCACTTCA AAAGATATCC GCACAGAGCT GGAGATGCAG 
ATGGTGTGTA ACCTTCGGGA GTTCAAGGAG TACATCGATA ACGAGATGAT CGTCATCTTG 
GGGCAGATGG ACAGGCCCAC AAAGATTTTT GAGCATGTTT ACCTGGGTTC TGAATGGAAT 
GCATCAAATC TGGAAGAACT GCAAAACACC GGGGTGCAAT ATATCCTTAA TGTGACGCGG 
GAGATCGATA ACTTCTTTCC AGGGCTATTT GAATACCACA ATATCCGGGT TTACGATGAA 
GAAGCCACAG ACTTGCTGGC TTACTGGAAT GACACTTACA AGTTCATTTC TAGGGCCAAA 
AAAGCCGGAG CGAAGTGTCT TGTGCACTGC AAGATGGGTG TTAGTCGTTC GGCCTCCACT 
GTTATCGCAT ACGCCATGAA GGAGTACGGC TGGGACATGG AGCAGGCCTT TGAGTACGTA 
AAAGAGCGGC GAGCCGTCAC CAAACCCAAC CCATCATTCA TGAGACAGCT TGTGGAGTAC 
CAGGGAATAC TGATAGCAAG CAAACAGAGA CACAATAAAT TGTGGCGTTC CCACTCAGAT 
AGTGATCTTT CGGAGCGACT CGAGCCACTC TGTAAAGCTA CTGCCGCACA GTCTCTAGGG 
CGGTCCAGTT CCCGCAATCA GACCTTCGGC CAGACTTCAC CCTCAATACA GGAGCTACTA 
CAGACTCTCA CCCCTTCCTC AGCTACTGCT ACTGGGTCTA ACAGACCGGT TGAGCCCAAC 
ACAACACGCT GGTCCGATAG CCACATTCAC AACGGAACCC TCCACGAAGC CACCGAGACG 
CTTTCCTTCC CCAGGCCCAG GGCTGCCACT GTGGTCCCAG AAGCCAGACC TGATATCACC 
TCTGTGACTG TTACTGAGAC GGTCTCACCA GGACGTCCTC ATCCGCCCCC TGCTGTACTT 
CACCCACCCT CGTCCCCCAA GCTTCTCTCA GATGCAAAGG CCAAGCAAGG CCTGCCTGAG 
GTGTCCAATC TTGTGCCTTT GGAGAAGTCT AGTCCAAAGG TGCACTCATC TGTAAACATT 
AGGACTCCTG AGCCATGCAC ACAGGCCCTG GACTCCCCAG GGCTCCAGGA TCTCAGCCAG 
CAAGTGCCTA ACATGGAGAC AGACTGTGCG GGGTTAAGTC ACAGCAGTGA TGAGGACCAC 
CACCCCAGTG CCACATTACA AAGGGCTGAG CTCACGCCCA CGCCTCCCTC CACTCTAAGC 
ATCGATCGCA TTGACTTCTT CAGTGCCAGA GAGAAGTTCC AGGGCCTTTC TCAAGAGATC 
CGCGGGACTG ACCAGGTGGT AGGGCAGGCG CCTCGGGACA AAAGTCCAAG TGTAGAGACC 
AAACGGCTGC TTCCTGACAA CCCCATCAAA GAGGAGGAAC AGAAAAAGCA AGGGAATAAT 
TTTCCAGAGA GAGAGCTGCA GTGTCTCAGT CCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293927.1
CDS	144..2459
Translation	MGVSSPLSDVKTLSGLETDLNTSTECLLAHDGIQILLQSGFMHQDNIKQFISESFLTVKGAALFLPRGNGSSAASSCSSRITAQRSKHAGDLQQHLQTMFTLLRPEDNIRLAVRLESTFPQCTRYMVVVSTNGRQDTEESVVLGMDFSPSDSSCSIGMVLPLWSDTRIHLDGDGGFSVSTDNKIHIFKPVSVQAMWSALQSLHKACEVARCHNYFPGSLFLTWVSYYQSRVSSDQARINEWNAMQDVQSHRADSPVLFSDVPTERERTERFIKTRLREIMMLKDLENVTSKDIRTELEMQMVCNLREFKEYIDNEMIVILGQMDRPTKIFEHVYLGSEWNASNLEELQNTGVQYILNVTREIDNFFPGLFEYHNIRVYDEEATDLLAYWNDTYKFISRAKKAGAKCLVHCKMGVSRSASTVIAYAMKEYGWDMEQAFEYVKERRAVTKPNPSFMRQLVEYQGILIASKQRHNKLWRSHSDSDLSERLEPLCKATAAQSLGRSSSRNQTFGQTSPSIQELLQTLTPSSATATGSNRPVEPNTTRWSDSHIHNGTLHEATETLSFPRPRAATVVPEARPDITSVTVTETVSPGRPHPPPAVLHPPSSPKLLSDAKAKQGLPEVSNLVPLEKSSPKVHSSVNIRTPEPCTQALDSPGLQDLSQQVPNMETDCAGLSHSSDEDHHPSATLQRAELTPTPPSTLSIDRIDFFSAREKFQGLSQEIRGTDQVVGQAPRDKSPSVETKRLLPDNPIKEEEQKKQGNNFPERELQCLSP

Target ORF information:

RefSeq Version	XM_009295652.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio slingshot protein phosphatase 2b (ssh2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295652.3

ORF Insert Sequence:

ATGGGGGTTT CCTCACCGCT GTCTGATGTA AAGACGTTAA GTGGTCTGGA GACTGATTTA 
AACACATCCA CAGAATGTCT GTTGGCTCAC GATGGGATAC AAATACTGCT GCAAAGTGGC 
TTCATGCACC AGGACAACAT CAAACAGTTC ATCAGTGAGA GCTTCCTAAC AGTCAAAGGT 
GCCGCCCTTT TCCTTCCACG TGGGAATGGC TCCTCTGCTG CCTCGTCCTG CTCCTCCAGA 
ATCACAGCAC AGCGCAGCAA ACATGCAGGG GACCTTCAGC AGCACCTGCA GACCATGTTC 
ACTTTACTGC GGCCCGAGGA TAACATCCGC TTGGCTGTGC GGTTGGAGAG CACCTTTCCC 
CAGTGCACCC GCTACATGGT GGTGGTCTCC ACTAATGGCA GGCAGGACAC AGAAGAGAGT 
GTGGTGCTGG GAATGGACTT CAGCCCCTCC GACAGTTCCT GTTCCATAGG CATGGTGCTT 
CCCTTGTGGA GTGACACACG GATCCATCTG GATGGTGACG GGGGCTTCAG TGTGTCTACA 
GATAACAAAA TACACATATT CAAGCCAGTG TCAGTTCAAG CCATGTGGTC TGCCTTGCAG 
TCGCTTCATA AGGCCTGTGA AGTGGCCCGC TGTCATAACT ACTTCCCGGG CAGCCTCTTC 
CTGACCTGGG TGAGTTACTA CCAGAGCCGG GTGTCCTCAG ATCAGGCCCG CATCAACGAG 
TGGAACGCCA TGCAGGACGT GCAGTCCCAC CGTGCAGACT CACCTGTGCT CTTCTCTGAT 
GTGCCAACTG AGCGGGAAAG AACAGAGAGA TTCATCAAGA CTCGATTAAG AGAGATCATG 
ATGCTAAAGG ATCTTGAAAA TGTCACTTCA AAAGATATCC GCACAGAGCT GGAGATGCAG 
ATGGTGTGTA ACCTTCGGGA GTTCAAGGAG TACATCGATA ACGAGATGAT CGTCATCTTG 
GGGCAGATGG ACAGGCCCAC AAAGATTTTT GAGCATGTTT ACCTGGGTTC TGAATGGAAT 
GCATCAAATC TGGAAGAACT GCAAAACACC GGGGTGCAAT ATATCCTTAA TGTGACGCGG 
GAGATCGATA ACTTCTTTCC AGGGCTATTT GAATACCACA ATATCCGGGT TTACGATGAA 
GAAGCCACAG ACTTGCTGGC TTACTGGAAT GACACTTACA AGTTCATTTC TAGGGCCAAA 
AAAGCCGGAG CGAAGTGTCT TGTGCACTGC AAGATGGGTG TTAGTCGTTC GGCCTCCACT 
GTTATCGCAT ACGCCATGAA GGAGTACGGC TGGGACATGG AGCAGGCCTT TGAGTACGTA 
AAAGAGCGGC GAGCCGTCAC CAAACCCAAC CCATCATTCA TGAGACAGCT TGTGGAGTAC 
CAGGGAATAC TGATAGCAAG CAAACAGAGA CACAATAAAT TGTGGCGTTC CCACTCAGAT 
AGTGATCTTT CGGAGCGACT CGAGCCACTC TGTAAAGCTA CTGCCGCACA GTCTCTAGGG 
CGGTCCAGTT CCCGCAATCA GACCTTCGGC CAGACTTCAC CCTCAATACA GGAGCTACTA 
CAGACTCTCA CCCCTTCCTC AGCTACTGCT ACTGGGTCTA ACAGACCGGT TGAGCCCAAC 
ACAACACGCT GGTCCGATAG CCACATTCAC AACGGAACCC TCCACGAAGC CACCGAGACG 
CTTTCCTTCC CCAGGCCCAG GGCTGCCACT GTGGTCCCAG AAGCCAGACC TGATATCACC 
TCTGTGACTG TTACTGAGAC GGTCTCACCA GGACGTCCTC ATCCGCCCCC TGCTGTACTT 
CACCCACCCT CGTCCCCCAA GCTTCTCTCA GATGCAAAGG CCAAGCAAGG CCTGCCTGAG 
GTGTCCAATC TTGTGCCTTT GGAGAAGTCT AGTCCAAAGG TGCACTCATC TGTAAACATT 
AGGACTCCTG AGCCATGCAC ACAGGCCCTG GACTCCCCAG GGCTCCAGGA TCTCAGCCAG 
CAAGTGCCTA ACATGGAGAC AGACTGTGCG GGGTTAAGTC ACAGCAGTGA TGAGGACCAC 
CACCCCAGTG CCACATTACA AAGGGCTGAG CTCACGCCCA CGCCTCCCTC CACTCTAAGC 
ATCGATCGCA TTGACTTCTT CAGTGCCAGA GAGAAGTTCC AGGGCCTTTC TCAAGAGATC 
CGCGGGACTG ACCAGGTGGT AGGGCAGGCG CCTCGGGACA AAAGTCCAAG TGTAGAGACC 
AAACGGCTGC TTCCTGACAA CCCCATCAAA GAGGAGGAAC AGAAAAAGCA AGGGAATAAT 
TTTCCAGAGA GAGAGCTGCA GTGTCTCAGT CCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ssh2b ( XM_009295652.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293927.1 Danio rerio slingshot protein phosphatase 2b (ssh2b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa42698
Clone ID Related Accession (Same CDS sequence)	XM_009295652.3 , XM_009295652.2
Accession Version	XM_009295652.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein phosphatase Slingshot homolog 2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295652.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGTTT CCTCACCGCT GTCTGATGTA AAGACGTTAA GTGGTCTGGA GACTGATTTA 
AACACATCCA CAGAATGTCT GTTGGCTCAC GATGGGATAC AAATACTGCT GCAAAGTGGC 
TTCATGCACC AGGACAACAT CAAACAGTTC ATCAGTGAGA GCTTCCTAAC AGTCAAAGGT 
GCCGCCCTTT TCCTTCCACG TGGGAATGGC TCCTCTGCTG CCTCGTCCTG CTCCTCCAGA 
ATCACAGCAC AGCGCAGCAA ACATGCAGGG GACCTTCAGC AGCACCTGCA GACCATGTTC 
ACTTTACTGC GGCCCGAGGA TAACATCCGC TTGGCTGTGC GGTTGGAGAG CACCTTTCCC 
CAGTGCACCC GCTACATGGT GGTGGTCTCC ACTAATGGCA GGCAGGACAC AGAAGAGAGT 
GTGGTGCTGG GAATGGACTT CAGCCCCTCC GACAGTTCCT GTTCCATAGG CATGGTGCTT 
CCCTTGTGGA GTGACACACG GATCCATCTG GATGGTGACG GGGGCTTCAG TGTGTCTACA 
GATAACAAAA TACACATATT CAAGCCAGTG TCAGTTCAAG CCATGTGGTC TGCCTTGCAG 
TCGCTTCATA AGGCCTGTGA AGTGGCCCGC TGTCATAACT ACTTCCCGGG CAGCCTCTTC 
CTGACCTGGG TGAGTTACTA CCAGAGCCGG GTGTCCTCAG ATCAGGCCCG CATCAACGAG 
TGGAACGCCA TGCAGGACGT GCAGTCCCAC CGTGCAGACT CACCTGTGCT CTTCTCTGAT 
GTGCCAACTG AGCGGGAAAG AACAGAGAGA TTCATCAAGA CTCGATTAAG AGAGATCATG 
ATGCTAAAGG ATCTTGAAAA TGTCACTTCA AAAGATATCC GCACAGAGCT GGAGATGCAG 
ATGGTGTGTA ACCTTCGGGA GTTCAAGGAG TACATCGATA ACGAGATGAT CGTCATCTTG 
GGGCAGATGG ACAGGCCCAC AAAGATTTTT GAGCATGTTT ACCTGGGTTC TGAATGGAAT 
GCATCAAATC TGGAAGAACT GCAAAACACC GGGGTGCAAT ATATCCTTAA TGTGACGCGG 
GAGATCGATA ACTTCTTTCC AGGGCTATTT GAATACCACA ATATCCGGGT TTACGATGAA 
GAAGCCACAG ACTTGCTGGC TTACTGGAAT GACACTTACA AGTTCATTTC TAGGGCCAAA 
AAAGCCGGAG CGAAGTGTCT TGTGCACTGC AAGATGGGTG TTAGTCGTTC GGCCTCCACT 
GTTATCGCAT ACGCCATGAA GGAGTACGGC TGGGACATGG AGCAGGCCTT TGAGTACGTA 
AAAGAGCGGC GAGCCGTCAC CAAACCCAAC CCATCATTCA TGAGACAGCT TGTGGAGTAC 
CAGGGAATAC TGATAGCAAG CAAACAGAGA CACAATAAAT TGTGGCGTTC CCACTCAGAT 
AGTGATCTTT CGGAGCGACT CGAGCCACTC TGTAAAGCTA CTGCCGCACA GTCTCTAGGG 
CGGTCCAGTT CCCGCAATCA GACCTTCGGC CAGACTTCAC CCTCAATACA GGAGCTACTA 
CAGACTCTCA CCCCTTCCTC AGCTACTGCT ACTGGGTCTA ACAGACCGGT TGAGCCCAAC 
ACAACACGCT GGTCCGATAG CCACATTCAC AACGGAACCC TCCACGAAGC CACCGAGACG 
CTTTCCTTCC CCAGGCCCAG GGCTGCCACT GTGGTCCCAG AAGCCAGACC TGATATCACC 
TCTGTGACTG TTACTGAGAC GGTCTCACCA GGACGTCCTC ATCCGCCCCC TGCTGTACTT 
CACCCACCCT CGTCCCCCAA GCTTCTCTCA GATGCAAAGG CCAAGCAAGG CCTGCCTGAG 
GTGTCCAATC TTGTGCCTTT GGAGAAGTCT AGTCCAAAGG TGCACTCATC TGTAAACATT 
AGGACTCCTG AGCCATGCAC ACAGGCCCTG GACTCCCCAG GGCTCCAGGA TCTCAGCCAG 
CAAGTGCCTA ACATGGAGAC AGACTGTGCG GGGTTAAGTC ACAGCAGTGA TGAGGACCAC 
CACCCCAGTG CCACATTACA AAGGGCTGAG CTCACGCCCA CGCCTCCCTC CACTCTAAGC 
ATCGATCGCA TTGACTTCTT CAGTGCCAGA GAGAAGTTCC AGGGCCTTTC TCAAGAGATC 
CGCGGGACTG ACCAGGTGGT AGGGCAGGCG CCTCGGGACA AAAGTCCAAG TGTAGAGACC 
AAACGGCTGC TTCCTGACAA CCCCATCAAA GAGGAGGAAC AGAAAAAGCA AGGGAATAAT 
TTTCCAGAGA GAGAGCTGCA GTGTCTCAGT CCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293927.1
CDS	119..2434
Translation	MGVSSPLSDVKTLSGLETDLNTSTECLLAHDGIQILLQSGFMHQDNIKQFISESFLTVKGAALFLPRGNGSSAASSCSSRITAQRSKHAGDLQQHLQTMFTLLRPEDNIRLAVRLESTFPQCTRYMVVVSTNGRQDTEESVVLGMDFSPSDSSCSIGMVLPLWSDTRIHLDGDGGFSVSTDNKIHIFKPVSVQAMWSALQSLHKACEVARCHNYFPGSLFLTWVSYYQSRVSSDQARINEWNAMQDVQSHRADSPVLFSDVPTERERTERFIKTRLREIMMLKDLENVTSKDIRTELEMQMVCNLREFKEYIDNEMIVILGQMDRPTKIFEHVYLGSEWNASNLEELQNTGVQYILNVTREIDNFFPGLFEYHNIRVYDEEATDLLAYWNDTYKFISRAKKAGAKCLVHCKMGVSRSASTVIAYAMKEYGWDMEQAFEYVKERRAVTKPNPSFMRQLVEYQGILIASKQRHNKLWRSHSDSDLSERLEPLCKATAAQSLGRSSSRNQTFGQTSPSIQELLQTLTPSSATATGSNRPVEPNTTRWSDSHIHNGTLHEATETLSFPRPRAATVVPEARPDITSVTVTETVSPGRPHPPPAVLHPPSSPKLLSDAKAKQGLPEVSNLVPLEKSSPKVHSSVNIRTPEPCTQALDSPGLQDLSQQVPNMETDCAGLSHSSDEDHHPSATLQRAELTPTPPSTLSIDRIDFFSAREKFQGLSQEIRGTDQVVGQAPRDKSPSVETKRLLPDNPIKEEEQKKQGNNFPERELQCLSP

Target ORF information:

RefSeq Version	XM_009295652.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio slingshot protein phosphatase 2b (ssh2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295652.2

ORF Insert Sequence:

ATGGGGGTTT CCTCACCGCT GTCTGATGTA AAGACGTTAA GTGGTCTGGA GACTGATTTA 
AACACATCCA CAGAATGTCT GTTGGCTCAC GATGGGATAC AAATACTGCT GCAAAGTGGC 
TTCATGCACC AGGACAACAT CAAACAGTTC ATCAGTGAGA GCTTCCTAAC AGTCAAAGGT 
GCCGCCCTTT TCCTTCCACG TGGGAATGGC TCCTCTGCTG CCTCGTCCTG CTCCTCCAGA 
ATCACAGCAC AGCGCAGCAA ACATGCAGGG GACCTTCAGC AGCACCTGCA GACCATGTTC 
ACTTTACTGC GGCCCGAGGA TAACATCCGC TTGGCTGTGC GGTTGGAGAG CACCTTTCCC 
CAGTGCACCC GCTACATGGT GGTGGTCTCC ACTAATGGCA GGCAGGACAC AGAAGAGAGT 
GTGGTGCTGG GAATGGACTT CAGCCCCTCC GACAGTTCCT GTTCCATAGG CATGGTGCTT 
CCCTTGTGGA GTGACACACG GATCCATCTG GATGGTGACG GGGGCTTCAG TGTGTCTACA 
GATAACAAAA TACACATATT CAAGCCAGTG TCAGTTCAAG CCATGTGGTC TGCCTTGCAG 
TCGCTTCATA AGGCCTGTGA AGTGGCCCGC TGTCATAACT ACTTCCCGGG CAGCCTCTTC 
CTGACCTGGG TGAGTTACTA CCAGAGCCGG GTGTCCTCAG ATCAGGCCCG CATCAACGAG 
TGGAACGCCA TGCAGGACGT GCAGTCCCAC CGTGCAGACT CACCTGTGCT CTTCTCTGAT 
GTGCCAACTG AGCGGGAAAG AACAGAGAGA TTCATCAAGA CTCGATTAAG AGAGATCATG 
ATGCTAAAGG ATCTTGAAAA TGTCACTTCA AAAGATATCC GCACAGAGCT GGAGATGCAG 
ATGGTGTGTA ACCTTCGGGA GTTCAAGGAG TACATCGATA ACGAGATGAT CGTCATCTTG 
GGGCAGATGG ACAGGCCCAC AAAGATTTTT GAGCATGTTT ACCTGGGTTC TGAATGGAAT 
GCATCAAATC TGGAAGAACT GCAAAACACC GGGGTGCAAT ATATCCTTAA TGTGACGCGG 
GAGATCGATA ACTTCTTTCC AGGGCTATTT GAATACCACA ATATCCGGGT TTACGATGAA 
GAAGCCACAG ACTTGCTGGC TTACTGGAAT GACACTTACA AGTTCATTTC TAGGGCCAAA 
AAAGCCGGAG CGAAGTGTCT TGTGCACTGC AAGATGGGTG TTAGTCGTTC GGCCTCCACT 
GTTATCGCAT ACGCCATGAA GGAGTACGGC TGGGACATGG AGCAGGCCTT TGAGTACGTA 
AAAGAGCGGC GAGCCGTCAC CAAACCCAAC CCATCATTCA TGAGACAGCT TGTGGAGTAC 
CAGGGAATAC TGATAGCAAG CAAACAGAGA CACAATAAAT TGTGGCGTTC CCACTCAGAT 
AGTGATCTTT CGGAGCGACT CGAGCCACTC TGTAAAGCTA CTGCCGCACA GTCTCTAGGG 
CGGTCCAGTT CCCGCAATCA GACCTTCGGC CAGACTTCAC CCTCAATACA GGAGCTACTA 
CAGACTCTCA CCCCTTCCTC AGCTACTGCT ACTGGGTCTA ACAGACCGGT TGAGCCCAAC 
ACAACACGCT GGTCCGATAG CCACATTCAC AACGGAACCC TCCACGAAGC CACCGAGACG 
CTTTCCTTCC CCAGGCCCAG GGCTGCCACT GTGGTCCCAG AAGCCAGACC TGATATCACC 
TCTGTGACTG TTACTGAGAC GGTCTCACCA GGACGTCCTC ATCCGCCCCC TGCTGTACTT 
CACCCACCCT CGTCCCCCAA GCTTCTCTCA GATGCAAAGG CCAAGCAAGG CCTGCCTGAG 
GTGTCCAATC TTGTGCCTTT GGAGAAGTCT AGTCCAAAGG TGCACTCATC TGTAAACATT 
AGGACTCCTG AGCCATGCAC ACAGGCCCTG GACTCCCCAG GGCTCCAGGA TCTCAGCCAG 
CAAGTGCCTA ACATGGAGAC AGACTGTGCG GGGTTAAGTC ACAGCAGTGA TGAGGACCAC 
CACCCCAGTG CCACATTACA AAGGGCTGAG CTCACGCCCA CGCCTCCCTC CACTCTAAGC 
ATCGATCGCA TTGACTTCTT CAGTGCCAGA GAGAAGTTCC AGGGCCTTTC TCAAGAGATC 
CGCGGGACTG ACCAGGTGGT AGGGCAGGCG CCTCGGGACA AAAGTCCAAG TGTAGAGACC 
AAACGGCTGC TTCCTGACAA CCCCATCAAA GAGGAGGAAC AGAAAAAGCA AGGGAATAAT 
TTTCCAGAGA GAGAGCTGCA GTGTCTCAGT CCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ssh2b ( XM_009295653.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293928.1 Danio rerio slingshot protein phosphatase 2b (ssh2b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa42699
Clone ID Related Accession (Same CDS sequence)	XM_009295653.2 , XM_009295653.3
Accession Version	XM_009295653.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein phosphatase Slingshot homolog 2 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295653.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGTTT CCTCACCGCT GTCTGATGTA AAGACGTTAA GTGGTCTGGA GACTGATTTA 
AACACATCCA CAGAATGTCT GTTGGCTCAC GATGGGATAC AAATACTGCT GCAAAGTGGC 
TTCATGCACC AGGACAACAT CAAACAGTTC ATCAGTGAGA GCTTCCTAAC AGTCAAAGGT 
GCCGCCCTTT TCCTTCCACG TGGGAATGGC TCCTCTGCTG CCTCGTCCTG CTCCTCCAGA 
ATCACAGCAC AGCGCAGCAA ACATGCAGGG GACCTTCAGC AGCACCTGCA GACCATGTTC 
ACTTTACTGC GGCCCGAGGA TAACATCCGC TTGGCTGTGC GGTTGGAGAG CACCTTTCCC 
CAGTGCACCC GCTACATGGT GGTGGTCTCC ACTAATGGCA GGCAGGACAC AGAAGAGAGT 
GTGGTGCTGG GAATGGACTT CAGCCCCTCC GACAGTTCCT GTTCCATAGG CATGGTGCTT 
CCCTTGTGGA GTGACACACG GATCCATCTG GATGGTGACG GGGGCTTCAG TGTGTCTACA 
GATAACAAAA TACACATATT CAAGCCAGTG TCAGTTCAAG CCATGTGGTC TGCCTTGCAG 
TCGCTTCATA AGGCCTGTGA AGTGGCCCGC TGTCATAACT ACTTCCCGGG CAGCCTCTTC 
CTGACCTGGG TGAGTTACTA CCAGAGCCGG GTGTCCTCAG ATCAGGCCCG CATCAACGAG 
TGGAACGCCA TGCAGGACGT GCAGTCCCAC CGTGCAGACT CACCTGTGCT CTTCTCTGAT 
GTGCCAACTG AGCGGGAAAG AACAGAGAGA TTCATCAAGA CTCGATTAAG AGAGATCATG 
ATGCTAAAGG ATCTTGAAAA TGTCACTTCA AAAGATATCC GCACAGAGCT GGAGATGCAG 
ATGGTGTGTA ACCTTCGGGA GTTCAAGGAG TACATCGATA ACGAGATGAT CGTCATCTTG 
GGGCAGATGG ACAGGCCCAC AAAGATTTTT GAGCATGTTT ACCTGGGTTC TGAATGGAAT 
GCATCAAATC TGGAAGAACT GCAAAACACC GGGGTGCAAT ATATCCTTAA TGTGACGCGG 
GAGATCGATA ACTTCTTTCC AGGGCTATTT GAATACCACA ATATCCGGGT TTACGATGAA 
GAAGCCACAG ACTTGCTGGC TTACTGGAAT GACACTTACA AGTTCATTTC TAGGGCCAAA 
AAAGCCGGAG CGAAGTGTCT TGTGCACTGC AAGATGGGTG TTAGTCGTTC GGCCTCCACT 
GTTATCGCAT ACGCCATGAA GGAGTACGGC TGGGACATGG AGCAGGCCTT TGAGTACGTA 
AAAGAGCGGC GAGCCGTCAC CAAACCCAAC CCATCATTCA TGAGACAGCT TGTGGAGTAC 
CAGGGAATAC TGATAGCAAG CAAACAGAGA CACAATAAAT TGTGGCGTTC CCACTCAGAT 
AGTGATCTTT CGGAGCGACT CGAGCCACTC TGTAAAGCTA CTGCCGCACA GTCTCTAGGG 
CGGTCCAGTT CCCGCAATCA GACCTTCGGC CAGACTTCAC CCTCAATACA GGAGCTACTA 
CAGACTCTCA CCCCTTCCTC AGCTACTGCT ACTGGGTCTA ACAGACCGGT TGAGCCCAAC 
ACAACACGCT GGTCCGATAG CCACATTCAC AACGGAACCC TCCACGAAGC CACCGAGACG 
CTTTCCTTCC CCAGGCCCAG GGCTGCCACT GTGGTCCCAG AAGCCAGACC TGATATCACC 
TCTGTGACTG TTACTGAGAC GGTCTCACCA GGACGTCCTC ATCCGCCCCC TGCTGTACTT 
CACCCACCCT CGTCCCCCAA GCTTCTCTCA GATGCAAAGG CCAAGCAAGG CCTGCCTGAG 
GTGTCCAATC TTGTGCCTTT GGAGAAGTCT AGTCCAAAGG TGCACTCATC TGTAAACATT 
AGGACTCCTG AGCCATGCAC ACAGGCCCTG GACTCCCCAG GGCTCCAGGA TCTCAGCCAG 
CAAGTGCCTA ACATGGAGAC AGACTGTGCG GGGTTAAGTC ACAGCAGTGA TGAGGACCAC 
CACCCCAGTG CCACATTACA AAGGGCTGAG CTCACGCCCA CGCCTCCCTC CACTCTAAGC 
ATCGATCGCA TTGACTTCTT CAGTGCCAGA GAGAAGTTCC AGGGCCTTTC TCAAGAGATC 
CGCGGGACTG ACCAGGTGGT AGGGCAGGCG CCTCGGGACA AAAGTCCAAG TGTAGAGACC 
AAACGGCTGC TTCCTGACAA CCCCATCAAA GAGGAGGAAC AGAAAAAGGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293928.1
CDS	119..2392
Translation	MGVSSPLSDVKTLSGLETDLNTSTECLLAHDGIQILLQSGFMHQDNIKQFISESFLTVKGAALFLPRGNGSSAASSCSSRITAQRSKHAGDLQQHLQTMFTLLRPEDNIRLAVRLESTFPQCTRYMVVVSTNGRQDTEESVVLGMDFSPSDSSCSIGMVLPLWSDTRIHLDGDGGFSVSTDNKIHIFKPVSVQAMWSALQSLHKACEVARCHNYFPGSLFLTWVSYYQSRVSSDQARINEWNAMQDVQSHRADSPVLFSDVPTERERTERFIKTRLREIMMLKDLENVTSKDIRTELEMQMVCNLREFKEYIDNEMIVILGQMDRPTKIFEHVYLGSEWNASNLEELQNTGVQYILNVTREIDNFFPGLFEYHNIRVYDEEATDLLAYWNDTYKFISRAKKAGAKCLVHCKMGVSRSASTVIAYAMKEYGWDMEQAFEYVKERRAVTKPNPSFMRQLVEYQGILIASKQRHNKLWRSHSDSDLSERLEPLCKATAAQSLGRSSSRNQTFGQTSPSIQELLQTLTPSSATATGSNRPVEPNTTRWSDSHIHNGTLHEATETLSFPRPRAATVVPEARPDITSVTVTETVSPGRPHPPPAVLHPPSSPKLLSDAKAKQGLPEVSNLVPLEKSSPKVHSSVNIRTPEPCTQALDSPGLQDLSQQVPNMETDCAGLSHSSDEDHHPSATLQRAELTPTPPSTLSIDRIDFFSAREKFQGLSQEIRGTDQVVGQAPRDKSPSVETKRLLPDNPIKEEEQKKV

Target ORF information:

RefSeq Version	XM_009295653.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio slingshot protein phosphatase 2b (ssh2b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295653.2

ORF Insert Sequence:

ATGGGGGTTT CCTCACCGCT GTCTGATGTA AAGACGTTAA GTGGTCTGGA GACTGATTTA 
AACACATCCA CAGAATGTCT GTTGGCTCAC GATGGGATAC AAATACTGCT GCAAAGTGGC 
TTCATGCACC AGGACAACAT CAAACAGTTC ATCAGTGAGA GCTTCCTAAC AGTCAAAGGT 
GCCGCCCTTT TCCTTCCACG TGGGAATGGC TCCTCTGCTG CCTCGTCCTG CTCCTCCAGA 
ATCACAGCAC AGCGCAGCAA ACATGCAGGG GACCTTCAGC AGCACCTGCA GACCATGTTC 
ACTTTACTGC GGCCCGAGGA TAACATCCGC TTGGCTGTGC GGTTGGAGAG CACCTTTCCC 
CAGTGCACCC GCTACATGGT GGTGGTCTCC ACTAATGGCA GGCAGGACAC AGAAGAGAGT 
GTGGTGCTGG GAATGGACTT CAGCCCCTCC GACAGTTCCT GTTCCATAGG CATGGTGCTT 
CCCTTGTGGA GTGACACACG GATCCATCTG GATGGTGACG GGGGCTTCAG TGTGTCTACA 
GATAACAAAA TACACATATT CAAGCCAGTG TCAGTTCAAG CCATGTGGTC TGCCTTGCAG 
TCGCTTCATA AGGCCTGTGA AGTGGCCCGC TGTCATAACT ACTTCCCGGG CAGCCTCTTC 
CTGACCTGGG TGAGTTACTA CCAGAGCCGG GTGTCCTCAG ATCAGGCCCG CATCAACGAG 
TGGAACGCCA TGCAGGACGT GCAGTCCCAC CGTGCAGACT CACCTGTGCT CTTCTCTGAT 
GTGCCAACTG AGCGGGAAAG AACAGAGAGA TTCATCAAGA CTCGATTAAG AGAGATCATG 
ATGCTAAAGG ATCTTGAAAA TGTCACTTCA AAAGATATCC GCACAGAGCT GGAGATGCAG 
ATGGTGTGTA ACCTTCGGGA GTTCAAGGAG TACATCGATA ACGAGATGAT CGTCATCTTG 
GGGCAGATGG ACAGGCCCAC AAAGATTTTT GAGCATGTTT ACCTGGGTTC TGAATGGAAT 
GCATCAAATC TGGAAGAACT GCAAAACACC GGGGTGCAAT ATATCCTTAA TGTGACGCGG 
GAGATCGATA ACTTCTTTCC AGGGCTATTT GAATACCACA ATATCCGGGT TTACGATGAA 
GAAGCCACAG ACTTGCTGGC TTACTGGAAT GACACTTACA AGTTCATTTC TAGGGCCAAA 
AAAGCCGGAG CGAAGTGTCT TGTGCACTGC AAGATGGGTG TTAGTCGTTC GGCCTCCACT 
GTTATCGCAT ACGCCATGAA GGAGTACGGC TGGGACATGG AGCAGGCCTT TGAGTACGTA 
AAAGAGCGGC GAGCCGTCAC CAAACCCAAC CCATCATTCA TGAGACAGCT TGTGGAGTAC 
CAGGGAATAC TGATAGCAAG CAAACAGAGA CACAATAAAT TGTGGCGTTC CCACTCAGAT 
AGTGATCTTT CGGAGCGACT CGAGCCACTC TGTAAAGCTA CTGCCGCACA GTCTCTAGGG 
CGGTCCAGTT CCCGCAATCA GACCTTCGGC CAGACTTCAC CCTCAATACA GGAGCTACTA 
CAGACTCTCA CCCCTTCCTC AGCTACTGCT ACTGGGTCTA ACAGACCGGT TGAGCCCAAC 
ACAACACGCT GGTCCGATAG CCACATTCAC AACGGAACCC TCCACGAAGC CACCGAGACG 
CTTTCCTTCC CCAGGCCCAG GGCTGCCACT GTGGTCCCAG AAGCCAGACC TGATATCACC 
TCTGTGACTG TTACTGAGAC GGTCTCACCA GGACGTCCTC ATCCGCCCCC TGCTGTACTT 
CACCCACCCT CGTCCCCCAA GCTTCTCTCA GATGCAAAGG CCAAGCAAGG CCTGCCTGAG 
GTGTCCAATC TTGTGCCTTT GGAGAAGTCT AGTCCAAAGG TGCACTCATC TGTAAACATT 
AGGACTCCTG AGCCATGCAC ACAGGCCCTG GACTCCCCAG GGCTCCAGGA TCTCAGCCAG 
CAAGTGCCTA ACATGGAGAC AGACTGTGCG GGGTTAAGTC ACAGCAGTGA TGAGGACCAC 
CACCCCAGTG CCACATTACA AAGGGCTGAG CTCACGCCCA CGCCTCCCTC CACTCTAAGC 
ATCGATCGCA TTGACTTCTT CAGTGCCAGA GAGAAGTTCC AGGGCCTTTC TCAAGAGATC 
CGCGGGACTG ACCAGGTGGT AGGGCAGGCG CCTCGGGACA AAAGTCCAAG TGTAGAGACC 
AAACGGCTGC TTCCTGACAA CCCCATCAAA GAGGAGGAAC AGAAAAAGGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ssh2b ( XM_009295653.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293928.1 Danio rerio slingshot protein phosphatase 2b (ssh2b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa42699
Clone ID Related Accession (Same CDS sequence)	XM_009295653.2 , XM_009295653.3
Accession Version	XM_009295653.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein phosphatase Slingshot homolog 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009295653.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGTTT CCTCACCGCT GTCTGATGTA AAGACGTTAA GTGGTCTGGA GACTGATTTA 
AACACATCCA CAGAATGTCT GTTGGCTCAC GATGGGATAC AAATACTGCT GCAAAGTGGC 
TTCATGCACC AGGACAACAT CAAACAGTTC ATCAGTGAGA GCTTCCTAAC AGTCAAAGGT 
GCCGCCCTTT TCCTTCCACG TGGGAATGGC TCCTCTGCTG CCTCGTCCTG CTCCTCCAGA 
ATCACAGCAC AGCGCAGCAA ACATGCAGGG GACCTTCAGC AGCACCTGCA GACCATGTTC 
ACTTTACTGC GGCCCGAGGA TAACATCCGC TTGGCTGTGC GGTTGGAGAG CACCTTTCCC 
CAGTGCACCC GCTACATGGT GGTGGTCTCC ACTAATGGCA GGCAGGACAC AGAAGAGAGT 
GTGGTGCTGG GAATGGACTT CAGCCCCTCC GACAGTTCCT GTTCCATAGG CATGGTGCTT 
CCCTTGTGGA GTGACACACG GATCCATCTG GATGGTGACG GGGGCTTCAG TGTGTCTACA 
GATAACAAAA TACACATATT CAAGCCAGTG TCAGTTCAAG CCATGTGGTC TGCCTTGCAG 
TCGCTTCATA AGGCCTGTGA AGTGGCCCGC TGTCATAACT ACTTCCCGGG CAGCCTCTTC 
CTGACCTGGG TGAGTTACTA CCAGAGCCGG GTGTCCTCAG ATCAGGCCCG CATCAACGAG 
TGGAACGCCA TGCAGGACGT GCAGTCCCAC CGTGCAGACT CACCTGTGCT CTTCTCTGAT 
GTGCCAACTG AGCGGGAAAG AACAGAGAGA TTCATCAAGA CTCGATTAAG AGAGATCATG 
ATGCTAAAGG ATCTTGAAAA TGTCACTTCA AAAGATATCC GCACAGAGCT GGAGATGCAG 
ATGGTGTGTA ACCTTCGGGA GTTCAAGGAG TACATCGATA ACGAGATGAT CGTCATCTTG 
GGGCAGATGG ACAGGCCCAC AAAGATTTTT GAGCATGTTT ACCTGGGTTC TGAATGGAAT 
GCATCAAATC TGGAAGAACT GCAAAACACC GGGGTGCAAT ATATCCTTAA TGTGACGCGG 
GAGATCGATA ACTTCTTTCC AGGGCTATTT GAATACCACA ATATCCGGGT TTACGATGAA 
GAAGCCACAG ACTTGCTGGC TTACTGGAAT GACACTTACA AGTTCATTTC TAGGGCCAAA 
AAAGCCGGAG CGAAGTGTCT TGTGCACTGC AAGATGGGTG TTAGTCGTTC GGCCTCCACT 
GTTATCGCAT ACGCCATGAA GGAGTACGGC TGGGACATGG AGCAGGCCTT TGAGTACGTA 
AAAGAGCGGC GAGCCGTCAC CAAACCCAAC CCATCATTCA TGAGACAGCT TGTGGAGTAC 
CAGGGAATAC TGATAGCAAG CAAACAGAGA CACAATAAAT TGTGGCGTTC CCACTCAGAT 
AGTGATCTTT CGGAGCGACT CGAGCCACTC TGTAAAGCTA CTGCCGCACA GTCTCTAGGG 
CGGTCCAGTT CCCGCAATCA GACCTTCGGC CAGACTTCAC CCTCAATACA GGAGCTACTA 
CAGACTCTCA CCCCTTCCTC AGCTACTGCT ACTGGGTCTA ACAGACCGGT TGAGCCCAAC 
ACAACACGCT GGTCCGATAG CCACATTCAC AACGGAACCC TCCACGAAGC CACCGAGACG 
CTTTCCTTCC CCAGGCCCAG GGCTGCCACT GTGGTCCCAG AAGCCAGACC TGATATCACC 
TCTGTGACTG TTACTGAGAC GGTCTCACCA GGACGTCCTC ATCCGCCCCC TGCTGTACTT 
CACCCACCCT CGTCCCCCAA GCTTCTCTCA GATGCAAAGG CCAAGCAAGG CCTGCCTGAG 
GTGTCCAATC TTGTGCCTTT GGAGAAGTCT AGTCCAAAGG TGCACTCATC TGTAAACATT 
AGGACTCCTG AGCCATGCAC ACAGGCCCTG GACTCCCCAG GGCTCCAGGA TCTCAGCCAG 
CAAGTGCCTA ACATGGAGAC AGACTGTGCG GGGTTAAGTC ACAGCAGTGA TGAGGACCAC 
CACCCCAGTG CCACATTACA AAGGGCTGAG CTCACGCCCA CGCCTCCCTC CACTCTAAGC 
ATCGATCGCA TTGACTTCTT CAGTGCCAGA GAGAAGTTCC AGGGCCTTTC TCAAGAGATC 
CGCGGGACTG ACCAGGTGGT AGGGCAGGCG CCTCGGGACA AAAGTCCAAG TGTAGAGACC 
AAACGGCTGC TTCCTGACAA CCCCATCAAA GAGGAGGAAC AGAAAAAGGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293928.1
CDS	144..2417
Translation	MGVSSPLSDVKTLSGLETDLNTSTECLLAHDGIQILLQSGFMHQDNIKQFISESFLTVKGAALFLPRGNGSSAASSCSSRITAQRSKHAGDLQQHLQTMFTLLRPEDNIRLAVRLESTFPQCTRYMVVVSTNGRQDTEESVVLGMDFSPSDSSCSIGMVLPLWSDTRIHLDGDGGFSVSTDNKIHIFKPVSVQAMWSALQSLHKACEVARCHNYFPGSLFLTWVSYYQSRVSSDQARINEWNAMQDVQSHRADSPVLFSDVPTERERTERFIKTRLREIMMLKDLENVTSKDIRTELEMQMVCNLREFKEYIDNEMIVILGQMDRPTKIFEHVYLGSEWNASNLEELQNTGVQYILNVTREIDNFFPGLFEYHNIRVYDEEATDLLAYWNDTYKFISRAKKAGAKCLVHCKMGVSRSASTVIAYAMKEYGWDMEQAFEYVKERRAVTKPNPSFMRQLVEYQGILIASKQRHNKLWRSHSDSDLSERLEPLCKATAAQSLGRSSSRNQTFGQTSPSIQELLQTLTPSSATATGSNRPVEPNTTRWSDSHIHNGTLHEATETLSFPRPRAATVVPEARPDITSVTVTETVSPGRPHPPPAVLHPPSSPKLLSDAKAKQGLPEVSNLVPLEKSSPKVHSSVNIRTPEPCTQALDSPGLQDLSQQVPNMETDCAGLSHSSDEDHHPSATLQRAELTPTPPSTLSIDRIDFFSAREKFQGLSQEIRGTDQVVGQAPRDKSPSVETKRLLPDNPIKEEEQKKV

Target ORF information:

RefSeq Version	XM_009295653.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio slingshot protein phosphatase 2b (ssh2b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295653.3

ORF Insert Sequence:

ATGGGGGTTT CCTCACCGCT GTCTGATGTA AAGACGTTAA GTGGTCTGGA GACTGATTTA 
AACACATCCA CAGAATGTCT GTTGGCTCAC GATGGGATAC AAATACTGCT GCAAAGTGGC 
TTCATGCACC AGGACAACAT CAAACAGTTC ATCAGTGAGA GCTTCCTAAC AGTCAAAGGT 
GCCGCCCTTT TCCTTCCACG TGGGAATGGC TCCTCTGCTG CCTCGTCCTG CTCCTCCAGA 
ATCACAGCAC AGCGCAGCAA ACATGCAGGG GACCTTCAGC AGCACCTGCA GACCATGTTC 
ACTTTACTGC GGCCCGAGGA TAACATCCGC TTGGCTGTGC GGTTGGAGAG CACCTTTCCC 
CAGTGCACCC GCTACATGGT GGTGGTCTCC ACTAATGGCA GGCAGGACAC AGAAGAGAGT 
GTGGTGCTGG GAATGGACTT CAGCCCCTCC GACAGTTCCT GTTCCATAGG CATGGTGCTT 
CCCTTGTGGA GTGACACACG GATCCATCTG GATGGTGACG GGGGCTTCAG TGTGTCTACA 
GATAACAAAA TACACATATT CAAGCCAGTG TCAGTTCAAG CCATGTGGTC TGCCTTGCAG 
TCGCTTCATA AGGCCTGTGA AGTGGCCCGC TGTCATAACT ACTTCCCGGG CAGCCTCTTC 
CTGACCTGGG TGAGTTACTA CCAGAGCCGG GTGTCCTCAG ATCAGGCCCG CATCAACGAG 
TGGAACGCCA TGCAGGACGT GCAGTCCCAC CGTGCAGACT CACCTGTGCT CTTCTCTGAT 
GTGCCAACTG AGCGGGAAAG AACAGAGAGA TTCATCAAGA CTCGATTAAG AGAGATCATG 
ATGCTAAAGG ATCTTGAAAA TGTCACTTCA AAAGATATCC GCACAGAGCT GGAGATGCAG 
ATGGTGTGTA ACCTTCGGGA GTTCAAGGAG TACATCGATA ACGAGATGAT CGTCATCTTG 
GGGCAGATGG ACAGGCCCAC AAAGATTTTT GAGCATGTTT ACCTGGGTTC TGAATGGAAT 
GCATCAAATC TGGAAGAACT GCAAAACACC GGGGTGCAAT ATATCCTTAA TGTGACGCGG 
GAGATCGATA ACTTCTTTCC AGGGCTATTT GAATACCACA ATATCCGGGT TTACGATGAA 
GAAGCCACAG ACTTGCTGGC TTACTGGAAT GACACTTACA AGTTCATTTC TAGGGCCAAA 
AAAGCCGGAG CGAAGTGTCT TGTGCACTGC AAGATGGGTG TTAGTCGTTC GGCCTCCACT 
GTTATCGCAT ACGCCATGAA GGAGTACGGC TGGGACATGG AGCAGGCCTT TGAGTACGTA 
AAAGAGCGGC GAGCCGTCAC CAAACCCAAC CCATCATTCA TGAGACAGCT TGTGGAGTAC 
CAGGGAATAC TGATAGCAAG CAAACAGAGA CACAATAAAT TGTGGCGTTC CCACTCAGAT 
AGTGATCTTT CGGAGCGACT CGAGCCACTC TGTAAAGCTA CTGCCGCACA GTCTCTAGGG 
CGGTCCAGTT CCCGCAATCA GACCTTCGGC CAGACTTCAC CCTCAATACA GGAGCTACTA 
CAGACTCTCA CCCCTTCCTC AGCTACTGCT ACTGGGTCTA ACAGACCGGT TGAGCCCAAC 
ACAACACGCT GGTCCGATAG CCACATTCAC AACGGAACCC TCCACGAAGC CACCGAGACG 
CTTTCCTTCC CCAGGCCCAG GGCTGCCACT GTGGTCCCAG AAGCCAGACC TGATATCACC 
TCTGTGACTG TTACTGAGAC GGTCTCACCA GGACGTCCTC ATCCGCCCCC TGCTGTACTT 
CACCCACCCT CGTCCCCCAA GCTTCTCTCA GATGCAAAGG CCAAGCAAGG CCTGCCTGAG 
GTGTCCAATC TTGTGCCTTT GGAGAAGTCT AGTCCAAAGG TGCACTCATC TGTAAACATT 
AGGACTCCTG AGCCATGCAC ACAGGCCCTG GACTCCCCAG GGCTCCAGGA TCTCAGCCAG 
CAAGTGCCTA ACATGGAGAC AGACTGTGCG GGGTTAAGTC ACAGCAGTGA TGAGGACCAC 
CACCCCAGTG CCACATTACA AAGGGCTGAG CTCACGCCCA CGCCTCCCTC CACTCTAAGC 
ATCGATCGCA TTGACTTCTT CAGTGCCAGA GAGAAGTTCC AGGGCCTTTC TCAAGAGATC 
CGCGGGACTG ACCAGGTGGT AGGGCAGGCG CCTCGGGACA AAAGTCCAAG TGTAGAGACC 
AAACGGCTGC TTCCTGACAA CCCCATCAAA GAGGAGGAAC AGAAAAAGGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ssh2b ( NM_001135103.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001128575.1 Danio rerio slingshot protein phosphatase 2b (ssh2b), mRNA.

Price & Availability↑

CloneID	ODa03810
Clone ID Related Accession (Same CDS sequence)	NM_001135103.1
Accession Version	NM_001135103.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-05
Organism	Danio rerio(zebrafish)
Product	protein phosphatase Slingshot homolog 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC168509.1. On Sep 21, 2008 this sequence version replaced XM_681067.3. ##Evidence-Data-START## Transcript exon combination :: BC168509.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCGTTGG TAACTGTGCA GCGGTCGCCT ACTCCAAGCG CAACATCCAG CCCGTGCGTT 
TCGGAGTCTG GCAGTGGGGA AGATGATCGC CGATCTCAAC CCAGAAGCAT CAGTGAGAGC 
TTCCTAACAG TCAAAGGTGC CGCCCTTTTC CTTCCACGTG GGAATGGCTC CTCTGCTGCC 
TCGTCCTGCT CCTCCAGAAT CACAGCACAG CGCAGCAAAC ATGCAGGGGA CCTTCAGCAG 
CACCTGCAGA CCATGTTCAC TTTACTGCGG CCCGAGGATA ACATCCGCTT GGCTGTGCGG 
TTGGAGAGCA CCTTTCCCCA GTGCACCCGC TACATGGTGG TGGTCTCCAC TAATGGCAGG 
CAGGACACAG AAGAGAGTGT GGTGCTGGGA ATGGACTTCA GCCCCTCCGA CAGTTCCTGT 
TCCATAGGCA TGGTGCTTCC CTTGTGGAGT GACACACGGA TCCATCTGGA TGGTGACGGG 
GGCTTCAGTG TGTCTACAGA TAACAAAATA CACATATTCA AGCCAGTGTC AGTTCAAGCC 
ATGTGGTCTG CCTTGCAGTC GCTTCATAAG GCCTGTGAAG TGGCCCGCTG TCATAACTAC 
TTCCCGGGCA GCCTCTTCCT GACCTGGGTG AGTTACTACC AGAGCCGGGT GTCCTCAGAT 
CAGGCCCGCA TCAACGAGTG GAACGCCATG CAGGACGTGC AGTCCCACCG TGCAGACTCA 
CCTGTGCTCT TCTCTGATGT GCCAACTGAG CGGGAAAGAA CAGAGAGATT CATCAAGACT 
CGATTAAGAG AGATCATGAT GCTAAAGGAT CTTGAAAATG TCACCTCAAA AGATATCCGC 
ACAGAGCTGG AGATGCAGAT GGTGTGTAAC CTTCGGGAGT TCAAGGAGTA CATCGATAAC 
GAGATGATCG TCATCTTGGG GCAGATGGAC AGGCCCACAA AGATTTTTGA GCATGTTTAC 
CTGGGTTCTG AATGGAATGC ATCAAATCTG GAAGAACTGC AAAACACCGG GGTGCAATAT 
ATCCTTAATG TGACGCGGGA GATCGATAAC TTCTTTCCAG GGCTATTTGA ATACCACAAT 
ATCCGGGTTT ACGATGAAGA AGCCACAGAC TTGCTGGCTT ACTGGAATGA CACTTACAAG 
TTCATTTCTA GGGCCAAAAA AGCCGGAGCG AAGTGTCTTG TGCACTGCAA GATGGGTGTT 
AGCCGTTCGG CCTCCACTGT TATCGCATAC GCCATGAAGG AGTACGGCTG GGACATGGAG 
CAGGCCTTTG AGTACGTAAA AGAGCGGCGA GCCGTCACCA AACCCAACCC ATCATTCATG 
AGACAGCTTG TGGAGTACCA GGGAATACTG ATAGCAAGCA AACAGAGACA CAATAAATTG 
TGGCGTTCCC ACTCAGATAG TGATCTTTCG GAGCGACTCG AGCCACTCTG TAAAGCTACT 
GCCGCACAGT CTCTAGGGCG GTCCAGTTCC CGCAATCAGA CCTTCGGCCA GACTTCACCC 
TCAATACAGG AGCTACTACA GACTCTCACC CCTTCCTCAG CTACTGCTAC TGGGCCTAAC 
GGACCGGTTG AGCCCAACAC AACACGCTGG TCCGATAGCC ACATTCACAA CGGAACCCTC 
CACGAAGCCA CAGAGACGCT TTCCTTCCCC AGGCCCAGGG CTGCCACTGT GGTCCCAGAA 
GCCAGACCTG ATATCACCTC TGTGACTGTT ACTGAGACGG TCTCACCAGG ACGTCCTCAT 
CCGCCCCCTG CTGTACTTCA CCCACCCTCG TCCCCCAAGC TTCTCTCAGA TGCAAAGGCC 
AAGCAAGGCC TGCCTGAGGT GTCCAATCTT GTGCCTTTGG AGAAGTCTAG TCCAAAGGTG 
CACTCATCTG TAAACATTAG GACTCCTGAG CCATGCACAC AGGCCCTGGA CTCCTCAGGG 
CTCCAGGATC TCAGCCAGCA AGTGCCTAAC ATGGAGACAG ACTGTGCGGG GTTAAGTCAC 
AGCAGTGATG AGGACCACCA CCCCAGTGCC ACATTACAAA GGGCTGAGCT CACGCCCACG 
CCTCCCTCCA CTCTAAGCAT CGATCGCATT GACTTCTTCA GTGCCAGAGA GAAGTTCCAG 
GGCCTTTCTC AAGAGATCCG CGGGACTGAC CAGGTGGTAG GGCAGGCGCC TCGGGACAAA 
AGTCCAAGTG TAGAGACCAA ACGGCTGCTT CCTGACAACC CCATCAAAGA GGAGGAACAG 
AAAAAGGAGA GCTGCAGTGT CTCAGTCCAT AGCAAAATGT CCGGAGTGGA GTCCACATGG 
CACAATGTAA CCCCTTCCCC CCCTCCAACC AGTCAACCTG TTTCCAGTAG CAGCAGTGAG 
GAAGAGGAGT TTGCTCAAGT CATGTCAGCA GAAGCTGAAA TGGAGAAGGT GGAGCATGGG 
AAAGACTGGC CTAAGGGCAC AGTGTGGCGA GCCACCCGGG AGCTGGAGCA AAAGATCAAG 
CAGGAGGTCT CGTCAGTAGC GCTGGTGTCC TCTGCTCCCG TGTGTCCCTC GCGACGCTCC 
CCCGTCAACA CCAGCAGCAT GGAGAAAACT GAGGAGACCT CATCCCACAC ACACCTCACA 
CCTGATCCAA ACACTGTGTC AACACGCCAT CACCAGCCTC AGTACCACAA GGTGGAGAAA 
GACCGAAGTT TTTCATTTGA AGAGGGAAGT GAAAGCGAGA GACGACTCAG GGAAGGAGAG 
CACAGGAACA CCGGCCTCAC ACACTCGAGA CAAAGCCATT CGCACTCGTC CAGTTGCTCT 
CCGCCTCTGG TTCTCCAGCT CGAGGGCATC ACAGAGCAGG AGAGTTACAC GGATTCTCGC 
TGTCCGAAGG ACATGAGGGA AACCTGGGAG ACCCTAAAAG AGCTCGGGGC GTTTCTCTGG 
CAGGTGAGCA ACTGCTCCGA CAGGAGGCTC AGCCAACCGT GGTCTAGGAG GGAGCAAAGG 
AAAGGCCGGG CTGCTCGAGA GAGGACCAAA GAGGTGGAGG CTCGGATCCG GCAGGCTGGA 
CTGACCCCAC CCTCAAAGAT GAAGCGTTCA GCCTCGTTGG CCAAGCTTGG GTCGCTGGAG 
ATCTCGCCTA TTGACCTGAG CGAACTTGAC TTGAGTCCTG CCTTACTCTC TGCTCCCACC 
AATAAGCCTC GGCCCAATTC GTACAGTGAC GATACCTCAA AAAAGCAGCG CGTTTTACCC 
CACTGCACTC CTGCTCCTCT CCCTTCCTCT TCTGCCTCCC CTGTTTCTTC CACTGGATCT 
CCTATGCTTG GACATGCAGA AAACGATGAC ACGACTGAAG AGGGGGAATA CAGTGACAAT 
GATGTCATGC CCATGCCACT GCCACACTCC CCGCCCTCCA ATCAGTGTTG GAGGGGGGGA 
AATGCACAGG AGGAGGCAGG ATGCGCCCCG TTACCTGCTT CGACCTTGAT ACCCGTGGCA 
ACACGCCAAC AATATGGGAG GACCCATCCC CTGCGGAGAC TGAAGAAACG GACCGTTAAC 
CCTTACCACA CTATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001128575.1
CDS	62..3559
Translation	MALVTVQRSPTPSATSSPCVSESGSGEDDRRSQPRSISESFLTVKGAALFLPRGNGSSAASSCSSRITAQRSKHAGDLQQHLQTMFTLLRPEDNIRLAVRLESTFPQCTRYMVVVSTNGRQDTEESVVLGMDFSPSDSSCSIGMVLPLWSDTRIHLDGDGGFSVSTDNKIHIFKPVSVQAMWSALQSLHKACEVARCHNYFPGSLFLTWVSYYQSRVSSDQARINEWNAMQDVQSHRADSPVLFSDVPTERERTERFIKTRLREIMMLKDLENVTSKDIRTELEMQMVCNLREFKEYIDNEMIVILGQMDRPTKIFEHVYLGSEWNASNLEELQNTGVQYILNVTREIDNFFPGLFEYHNIRVYDEEATDLLAYWNDTYKFISRAKKAGAKCLVHCKMGVSRSASTVIAYAMKEYGWDMEQAFEYVKERRAVTKPNPSFMRQLVEYQGILIASKQRHNKLWRSHSDSDLSERLEPLCKATAAQSLGRSSSRNQTFGQTSPSIQELLQTLTPSSATATGPNGPVEPNTTRWSDSHIHNGTLHEATETLSFPRPRAATVVPEARPDITSVTVTETVSPGRPHPPPAVLHPPSSPKLLSDAKAKQGLPEVSNLVPLEKSSPKVHSSVNIRTPEPCTQALDSSGLQDLSQQVPNMETDCAGLSHSSDEDHHPSATLQRAELTPTPPSTLSIDRIDFFSAREKFQGLSQEIRGTDQVVGQAPRDKSPSVETKRLLPDNPIKEEEQKKESCSVSVHSKMSGVESTWHNVTPSPPPTSQPVSSSSSEEEEFAQVMSAEAEMEKVEHGKDWPKGTVWRATRELEQKIKQEVSSVALVSSAPVCPSRRSPVNTSSMEKTEETSSHTHLTPDPNTVSTRHHQPQYHKVEKDRSFSFEEGSESERRLREGEHRNTGLTHSRQSHSHSSSCSPPLVLQLEGITEQESYTDSRCPKDMRETWETLKELGAFLWQVSNCSDRRLSQPWSRREQRKGRAARERTKEVEARIRQAGLTPPSKMKRSASLAKLGSLEISPIDLSELDLSPALLSAPTNKPRPNSYSDDTSKKQRVLPHCTPAPLPSSSASPVSSTGSPMLGHAENDDTTEEGEYSDNDVMPMPLPHSPPSNQCWRGGNAQEEAGCAPLPASTLIPVATRQQYGRTHPLRRLKKRTVNPYHTM

Target ORF information:

RefSeq Version	NM_001135103.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio slingshot protein phosphatase 2b (ssh2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135103.1

ORF Insert Sequence:

ATGGCGTTGG TAACTGTGCA GCGGTCGCCT ACTCCAAGCG CAACATCCAG CCCGTGCGTT 
TCGGAGTCTG GCAGTGGGGA AGATGATCGC CGATCTCAAC CCAGAAGCAT CAGTGAGAGC 
TTCCTAACAG TCAAAGGTGC CGCCCTTTTC CTTCCACGTG GGAATGGCTC CTCTGCTGCC 
TCGTCCTGCT CCTCCAGAAT CACAGCACAG CGCAGCAAAC ATGCAGGGGA CCTTCAGCAG 
CACCTGCAGA CCATGTTCAC TTTACTGCGG CCCGAGGATA ACATCCGCTT GGCTGTGCGG 
TTGGAGAGCA CCTTTCCCCA GTGCACCCGC TACATGGTGG TGGTCTCCAC TAATGGCAGG 
CAGGACACAG AAGAGAGTGT GGTGCTGGGA ATGGACTTCA GCCCCTCCGA CAGTTCCTGT 
TCCATAGGCA TGGTGCTTCC CTTGTGGAGT GACACACGGA TCCATCTGGA TGGTGACGGG 
GGCTTCAGTG TGTCTACAGA TAACAAAATA CACATATTCA AGCCAGTGTC AGTTCAAGCC 
ATGTGGTCTG CCTTGCAGTC GCTTCATAAG GCCTGTGAAG TGGCCCGCTG TCATAACTAC 
TTCCCGGGCA GCCTCTTCCT GACCTGGGTG AGTTACTACC AGAGCCGGGT GTCCTCAGAT 
CAGGCCCGCA TCAACGAGTG GAACGCCATG CAGGACGTGC AGTCCCACCG TGCAGACTCA 
CCTGTGCTCT TCTCTGATGT GCCAACTGAG CGGGAAAGAA CAGAGAGATT CATCAAGACT 
CGATTAAGAG AGATCATGAT GCTAAAGGAT CTTGAAAATG TCACCTCAAA AGATATCCGC 
ACAGAGCTGG AGATGCAGAT GGTGTGTAAC CTTCGGGAGT TCAAGGAGTA CATCGATAAC 
GAGATGATCG TCATCTTGGG GCAGATGGAC AGGCCCACAA AGATTTTTGA GCATGTTTAC 
CTGGGTTCTG AATGGAATGC ATCAAATCTG GAAGAACTGC AAAACACCGG GGTGCAATAT 
ATCCTTAATG TGACGCGGGA GATCGATAAC TTCTTTCCAG GGCTATTTGA ATACCACAAT 
ATCCGGGTTT ACGATGAAGA AGCCACAGAC TTGCTGGCTT ACTGGAATGA CACTTACAAG 
TTCATTTCTA GGGCCAAAAA AGCCGGAGCG AAGTGTCTTG TGCACTGCAA GATGGGTGTT 
AGCCGTTCGG CCTCCACTGT TATCGCATAC GCCATGAAGG AGTACGGCTG GGACATGGAG 
CAGGCCTTTG AGTACGTAAA AGAGCGGCGA GCCGTCACCA AACCCAACCC ATCATTCATG 
AGACAGCTTG TGGAGTACCA GGGAATACTG ATAGCAAGCA AACAGAGACA CAATAAATTG 
TGGCGTTCCC ACTCAGATAG TGATCTTTCG GAGCGACTCG AGCCACTCTG TAAAGCTACT 
GCCGCACAGT CTCTAGGGCG GTCCAGTTCC CGCAATCAGA CCTTCGGCCA GACTTCACCC 
TCAATACAGG AGCTACTACA GACTCTCACC CCTTCCTCAG CTACTGCTAC TGGGCCTAAC 
GGACCGGTTG AGCCCAACAC AACACGCTGG TCCGATAGCC ACATTCACAA CGGAACCCTC 
CACGAAGCCA CAGAGACGCT TTCCTTCCCC AGGCCCAGGG CTGCCACTGT GGTCCCAGAA 
GCCAGACCTG ATATCACCTC TGTGACTGTT ACTGAGACGG TCTCACCAGG ACGTCCTCAT 
CCGCCCCCTG CTGTACTTCA CCCACCCTCG TCCCCCAAGC TTCTCTCAGA TGCAAAGGCC 
AAGCAAGGCC TGCCTGAGGT GTCCAATCTT GTGCCTTTGG AGAAGTCTAG TCCAAAGGTG 
CACTCATCTG TAAACATTAG GACTCCTGAG CCATGCACAC AGGCCCTGGA CTCCTCAGGG 
CTCCAGGATC TCAGCCAGCA AGTGCCTAAC ATGGAGACAG ACTGTGCGGG GTTAAGTCAC 
AGCAGTGATG AGGACCACCA CCCCAGTGCC ACATTACAAA GGGCTGAGCT CACGCCCACG 
CCTCCCTCCA CTCTAAGCAT CGATCGCATT GACTTCTTCA GTGCCAGAGA GAAGTTCCAG 
GGCCTTTCTC AAGAGATCCG CGGGACTGAC CAGGTGGTAG GGCAGGCGCC TCGGGACAAA 
AGTCCAAGTG TAGAGACCAA ACGGCTGCTT CCTGACAACC CCATCAAAGA GGAGGAACAG 
AAAAAGGAGA GCTGCAGTGT CTCAGTCCAT AGCAAAATGT CCGGAGTGGA GTCCACATGG 
CACAATGTAA CCCCTTCCCC CCCTCCAACC AGTCAACCTG TTTCCAGTAG CAGCAGTGAG 
GAAGAGGAGT TTGCTCAAGT CATGTCAGCA GAAGCTGAAA TGGAGAAGGT GGAGCATGGG 
AAAGACTGGC CTAAGGGCAC AGTGTGGCGA GCCACCCGGG AGCTGGAGCA AAAGATCAAG 
CAGGAGGTCT CGTCAGTAGC GCTGGTGTCC TCTGCTCCCG TGTGTCCCTC GCGACGCTCC 
CCCGTCAACA CCAGCAGCAT GGAGAAAACT GAGGAGACCT CATCCCACAC ACACCTCACA 
CCTGATCCAA ACACTGTGTC AACACGCCAT CACCAGCCTC AGTACCACAA GGTGGAGAAA 
GACCGAAGTT TTTCATTTGA AGAGGGAAGT GAAAGCGAGA GACGACTCAG GGAAGGAGAG 
CACAGGAACA CCGGCCTCAC ACACTCGAGA CAAAGCCATT CGCACTCGTC CAGTTGCTCT 
CCGCCTCTGG TTCTCCAGCT CGAGGGCATC ACAGAGCAGG AGAGTTACAC GGATTCTCGC 
TGTCCGAAGG ACATGAGGGA AACCTGGGAG ACCCTAAAAG AGCTCGGGGC GTTTCTCTGG 
CAGGTGAGCA ACTGCTCCGA CAGGAGGCTC AGCCAACCGT GGTCTAGGAG GGAGCAAAGG 
AAAGGCCGGG CTGCTCGAGA GAGGACCAAA GAGGTGGAGG CTCGGATCCG GCAGGCTGGA 
CTGACCCCAC CCTCAAAGAT GAAGCGTTCA GCCTCGTTGG CCAAGCTTGG GTCGCTGGAG 
ATCTCGCCTA TTGACCTGAG CGAACTTGAC TTGAGTCCTG CCTTACTCTC TGCTCCCACC 
AATAAGCCTC GGCCCAATTC GTACAGTGAC GATACCTCAA AAAAGCAGCG CGTTTTACCC 
CACTGCACTC CTGCTCCTCT CCCTTCCTCT TCTGCCTCCC CTGTTTCTTC CACTGGATCT 
CCTATGCTTG GACATGCAGA AAACGATGAC ACGACTGAAG AGGGGGAATA CAGTGACAAT 
GATGTCATGC CCATGCCACT GCCACACTCC CCGCCCTCCA ATCAGTGTTG GAGGGGGGGA 
AATGCACAGG AGGAGGCAGG ATGCGCCCCG TTACCTGCTT CGACCTTGAT ACCCGTGGCA 
ACACGCCAAC AATATGGGAG GACCCATCCC CTGCGGAGAC TGAAGAAACG GACCGTTAAC 
CCTTACCACA CTATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ssh2b ( XM_009295650.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293925.1 Danio rerio slingshot protein phosphatase 2b (ssh2b), transcript variant X1, mRNA.

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CloneID	ODa26174
Clone ID Related Accession (Same CDS sequence)	XM_009295650.2 , XM_009295650.3
Accession Version	XM_009295650.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein phosphatase Slingshot homolog 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295650.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGTTT CCTCACCGCT GTCTGATGTA AAGACGTTAA GTGGTCTGGA GACTGATTTA 
AACACATCCA CAGAATGTCT GTTGGCTCAC GATGGGATAC AAATACTGCT GCAAAGTGGC 
TTCATGCACC AGGACAACAT CAAACAGTTC ATCAGTGAGA GCTTCCTAAC AGTCAAAGGT 
GCCGCCCTTT TCCTTCCACG TGGGAATGGC TCCTCTGCTG CCTCGTCCTG CTCCTCCAGA 
ATCACAGCAC AGCGCAGCAA ACATGCAGGG GACCTTCAGC AGCACCTGCA GACCATGTTC 
ACTTTACTGC GGCCCGAGGA TAACATCCGC TTGGCTGTGC GGTTGGAGAG CACCTTTCCC 
CAGTGCACCC GCTACATGGT GGTGGTCTCC ACTAATGGCA GGCAGGACAC AGAAGAGAGT 
GTGGTGCTGG GAATGGACTT CAGCCCCTCC GACAGTTCCT GTTCCATAGG CATGGTGCTT 
CCCTTGTGGA GTGACACACG GATCCATCTG GATGGTGACG GGGGCTTCAG TGTGTCTACA 
GATAACAAAA TACACATATT CAAGCCAGTG TCAGTTCAAG CCATGTGGTC TGCCTTGCAG 
TCGCTTCATA AGGCCTGTGA AGTGGCCCGC TGTCATAACT ACTTCCCGGG CAGCCTCTTC 
CTGACCTGGG TGAGTTACTA CCAGAGCCGG GTGTCCTCAG ATCAGGCCCG CATCAACGAG 
TGGAACGCCA TGCAGGACGT GCAGTCCCAC CGTGCAGACT CACCTGTGCT CTTCTCTGAT 
GTGCCAACTG AGCGGGAAAG AACAGAGAGA TTCATCAAGA CTCGATTAAG AGAGATCATG 
ATGCTAAAGG ATCTTGAAAA TGTCACTTCA AAAGATATCC GCACAGAGCT GGAGATGCAG 
ATGGTGTGTA ACCTTCGGGA GTTCAAGGAG TACATCGATA ACGAGATGAT CGTCATCTTG 
GGGCAGATGG ACAGGCCCAC AAAGATTTTT GAGCATGTTT ACCTGGGTTC TGAATGGAAT 
GCATCAAATC TGGAAGAACT GCAAAACACC GGGGTGCAAT ATATCCTTAA TGTGACGCGG 
GAGATCGATA ACTTCTTTCC AGGGCTATTT GAATACCACA ATATCCGGGT TTACGATGAA 
GAAGCCACAG ACTTGCTGGC TTACTGGAAT GACACTTACA AGTTCATTTC TAGGGCCAAA 
AAAGCCGGAG CGAAGTGTCT TGTGCACTGC AAGATGGGTG TTAGTCGTTC GGCCTCCACT 
GTTATCGCAT ACGCCATGAA GGAGTACGGC TGGGACATGG AGCAGGCCTT TGAGTACGTA 
AAAGAGCGGC GAGCCGTCAC CAAACCCAAC CCATCATTCA TGAGACAGCT TGTGGAGTAC 
CAGGGAATAC TGATAGCAAG CAAACAGAGA CACAATAAAT TGTGGCGTTC CCACTCAGAT 
AGTGATCTTT CGGAGCGACT CGAGCCACTC TGTAAAGCTA CTGCCGCACA GTCTCTAGGG 
CGGTCCAGTT CCCGCAATCA GACCTTCGGC CAGACTTCAC CCTCAATACA GGAGCTACTA 
CAGACTCTCA CCCCTTCCTC AGCTACTGCT ACTGGGTCTA ACAGACCGGT TGAGCCCAAC 
ACAACACGCT GGTCCGATAG CCACATTCAC AACGGAACCC TCCACGAAGC CACCGAGACG 
CTTTCCTTCC CCAGGCCCAG GGCTGCCACT GTGGTCCCAG AAGCCAGACC TGATATCACC 
TCTGTGACTG TTACTGAGAC GGTCTCACCA GGACGTCCTC ATCCGCCCCC TGCTGTACTT 
CACCCACCCT CGTCCCCCAA GCTTCTCTCA GATGCAAAGG CCAAGCAAGG CCTGCCTGAG 
GTGTCCAATC TTGTGCCTTT GGAGAAGTCT AGTCCAAAGG TGCACTCATC TGTAAACATT 
AGGACTCCTG AGCCATGCAC ACAGGCCCTG GACTCCCCAG GGCTCCAGGA TCTCAGCCAG 
CAAGTGCCTA ACATGGAGAC AGACTGTGCG GGGTTAAGTC ACAGCAGTGA TGAGGACCAC 
CACCCCAGTG CCACATTACA AAGGGCTGAG CTCACGCCCA CGCCTCCCTC CACTCTAAGC 
ATCGATCGCA TTGACTTCTT CAGTGCCAGA GAGAAGTTCC AGGGCCTTTC TCAAGAGATC 
CGCGGGACTG ACCAGGTGGT AGGGCAGGCG CCTCGGGACA AAAGTCCAAG TGTAGAGACC 
AAACGGCTGC TTCCTGACAA CCCCATCAAA GAGGAGGAAC AGAAAAAGGA GAGCTGCAGT 
GTCTCAGTCC ATAGCAAAAT GTCCGGAGTG GAGTCCACAT GGCACAATGT AACCCCTTCC 
CCCCCTCCAA CCAGTCAACC TGTTTCCAGT AGCAGCAGTG AGGAAGAGGA GTTTGCTCAA 
GTCATGTCAG CAGAAGCTGA AATGGAGAAG GTGGAGCATG GGAAAGACTG GCCTAAGGGC 
ACAGTGTGGC GAGCCACCCG GGAGCTGGAG CAAAAGATCA AGCAGGAGGT CTCGTCAGTA 
GCGCTGGTGT CCTCTGCTCC CGTGTGTCCC TCGCGACGCT CCCCCGTCAA CACCAGCAGC 
ATGGAGAAAA CTGAGGAGAC CTCATCCCAC ACACACCTCA CACCTGATCC AAACACTGTG 
TCAACACGCC ATCACCAGCC TCAGTACCAC AAGGTGGAGA AAGACCGGAG TTTTTCATTT 
GAAGAGGGAA GTGAAAGCGA GAGACGACTC AGGGAAGGAG AGCACAGGAA CACCGGCCTC 
ACACACTCGA GACAAAGCCA TTCGCACTCG TCCAGTTGCT CTCCGCCTCT GGTTCTCCAG 
CTCGAGGGCA TCACAGAGCA GGAGAGTTAC ACGGATTCTC GCTGTCCGAA GGACATGAGG 
GAAACCTGGG AGACCCTAAA AGAGCTCGGG GCGTTTCTCT GGCAGGTGAG CAACTGCTCC 
GACAGGAGGC TCAGCCAACC GTGGTCTAGG AGGGAGCAAA GGAAAGGCCG GGCTGCTCGA 
GAGAGGACCA AAGAGGTGGA GGCTCGGATC CGGCAGGCTG GACTGACCCC ACCCTCAAAG 
ATGAAGCGTT CAGCCTCGTT GGCCAAGCTT GGGTCGCTGG AGATCTCGCC TATTGACCTG 
AGCGAACTTG ACTTGAGTCC TGCCTTACTC TCTGCTCCCA CCAATAAGCC TCGGCCCAAT 
TCATACAGTG ACGATACCTC AAAAAAGCAG CGCGTTTTAC CCCACTGCAC TCCTGCTCCC 
CTCCCTTCCT CTTCTGCCTC CCCTGTTTCT TCCACTGGAT CTCCTATGCT TGGACATGCA 
GAAAACGATG ACACGACTGA AGAGGGGGAA TACAGTGACA ATGATGTCAT GCCCATGCCA 
CTGCCACACT CCCCGCCCTC CAATCAGTGT TGGAGGGGGG GAAATGCACA GGAGGAGGCA 
GGATGCGCCC CGTTACCTGC TTCGACCTTG ATACCTGTGG CAACACGCCA ACAATATGGG 
AGGACCCATC CCCTGCGGAG ACTGAAGAAA CGGACCGTTA ACCCTTACCA CACTATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293925.1
CDS	119..3658
Translation	MGVSSPLSDVKTLSGLETDLNTSTECLLAHDGIQILLQSGFMHQDNIKQFISESFLTVKGAALFLPRGNGSSAASSCSSRITAQRSKHAGDLQQHLQTMFTLLRPEDNIRLAVRLESTFPQCTRYMVVVSTNGRQDTEESVVLGMDFSPSDSSCSIGMVLPLWSDTRIHLDGDGGFSVSTDNKIHIFKPVSVQAMWSALQSLHKACEVARCHNYFPGSLFLTWVSYYQSRVSSDQARINEWNAMQDVQSHRADSPVLFSDVPTERERTERFIKTRLREIMMLKDLENVTSKDIRTELEMQMVCNLREFKEYIDNEMIVILGQMDRPTKIFEHVYLGSEWNASNLEELQNTGVQYILNVTREIDNFFPGLFEYHNIRVYDEEATDLLAYWNDTYKFISRAKKAGAKCLVHCKMGVSRSASTVIAYAMKEYGWDMEQAFEYVKERRAVTKPNPSFMRQLVEYQGILIASKQRHNKLWRSHSDSDLSERLEPLCKATAAQSLGRSSSRNQTFGQTSPSIQELLQTLTPSSATATGSNRPVEPNTTRWSDSHIHNGTLHEATETLSFPRPRAATVVPEARPDITSVTVTETVSPGRPHPPPAVLHPPSSPKLLSDAKAKQGLPEVSNLVPLEKSSPKVHSSVNIRTPEPCTQALDSPGLQDLSQQVPNMETDCAGLSHSSDEDHHPSATLQRAELTPTPPSTLSIDRIDFFSAREKFQGLSQEIRGTDQVVGQAPRDKSPSVETKRLLPDNPIKEEEQKKESCSVSVHSKMSGVESTWHNVTPSPPPTSQPVSSSSSEEEEFAQVMSAEAEMEKVEHGKDWPKGTVWRATRELEQKIKQEVSSVALVSSAPVCPSRRSPVNTSSMEKTEETSSHTHLTPDPNTVSTRHHQPQYHKVEKDRSFSFEEGSESERRLREGEHRNTGLTHSRQSHSHSSSCSPPLVLQLEGITEQESYTDSRCPKDMRETWETLKELGAFLWQVSNCSDRRLSQPWSRREQRKGRAARERTKEVEARIRQAGLTPPSKMKRSASLAKLGSLEISPIDLSELDLSPALLSAPTNKPRPNSYSDDTSKKQRVLPHCTPAPLPSSSASPVSSTGSPMLGHAENDDTTEEGEYSDNDVMPMPLPHSPPSNQCWRGGNAQEEAGCAPLPASTLIPVATRQQYGRTHPLRRLKKRTVNPYHTM

Target ORF information:

RefSeq Version	XM_009295650.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio slingshot protein phosphatase 2b (ssh2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295650.2

ORF Insert Sequence:

ATGGGGGTTT CCTCACCGCT GTCTGATGTA AAGACGTTAA GTGGTCTGGA GACTGATTTA 
AACACATCCA CAGAATGTCT GTTGGCTCAC GATGGGATAC AAATACTGCT GCAAAGTGGC 
TTCATGCACC AGGACAACAT CAAACAGTTC ATCAGTGAGA GCTTCCTAAC AGTCAAAGGT 
GCCGCCCTTT TCCTTCCACG TGGGAATGGC TCCTCTGCTG CCTCGTCCTG CTCCTCCAGA 
ATCACAGCAC AGCGCAGCAA ACATGCAGGG GACCTTCAGC AGCACCTGCA GACCATGTTC 
ACTTTACTGC GGCCCGAGGA TAACATCCGC TTGGCTGTGC GGTTGGAGAG CACCTTTCCC 
CAGTGCACCC GCTACATGGT GGTGGTCTCC ACTAATGGCA GGCAGGACAC AGAAGAGAGT 
GTGGTGCTGG GAATGGACTT CAGCCCCTCC GACAGTTCCT GTTCCATAGG CATGGTGCTT 
CCCTTGTGGA GTGACACACG GATCCATCTG GATGGTGACG GGGGCTTCAG TGTGTCTACA 
GATAACAAAA TACACATATT CAAGCCAGTG TCAGTTCAAG CCATGTGGTC TGCCTTGCAG 
TCGCTTCATA AGGCCTGTGA AGTGGCCCGC TGTCATAACT ACTTCCCGGG CAGCCTCTTC 
CTGACCTGGG TGAGTTACTA CCAGAGCCGG GTGTCCTCAG ATCAGGCCCG CATCAACGAG 
TGGAACGCCA TGCAGGACGT GCAGTCCCAC CGTGCAGACT CACCTGTGCT CTTCTCTGAT 
GTGCCAACTG AGCGGGAAAG AACAGAGAGA TTCATCAAGA CTCGATTAAG AGAGATCATG 
ATGCTAAAGG ATCTTGAAAA TGTCACTTCA AAAGATATCC GCACAGAGCT GGAGATGCAG 
ATGGTGTGTA ACCTTCGGGA GTTCAAGGAG TACATCGATA ACGAGATGAT CGTCATCTTG 
GGGCAGATGG ACAGGCCCAC AAAGATTTTT GAGCATGTTT ACCTGGGTTC TGAATGGAAT 
GCATCAAATC TGGAAGAACT GCAAAACACC GGGGTGCAAT ATATCCTTAA TGTGACGCGG 
GAGATCGATA ACTTCTTTCC AGGGCTATTT GAATACCACA ATATCCGGGT TTACGATGAA 
GAAGCCACAG ACTTGCTGGC TTACTGGAAT GACACTTACA AGTTCATTTC TAGGGCCAAA 
AAAGCCGGAG CGAAGTGTCT TGTGCACTGC AAGATGGGTG TTAGTCGTTC GGCCTCCACT 
GTTATCGCAT ACGCCATGAA GGAGTACGGC TGGGACATGG AGCAGGCCTT TGAGTACGTA 
AAAGAGCGGC GAGCCGTCAC CAAACCCAAC CCATCATTCA TGAGACAGCT TGTGGAGTAC 
CAGGGAATAC TGATAGCAAG CAAACAGAGA CACAATAAAT TGTGGCGTTC CCACTCAGAT 
AGTGATCTTT CGGAGCGACT CGAGCCACTC TGTAAAGCTA CTGCCGCACA GTCTCTAGGG 
CGGTCCAGTT CCCGCAATCA GACCTTCGGC CAGACTTCAC CCTCAATACA GGAGCTACTA 
CAGACTCTCA CCCCTTCCTC AGCTACTGCT ACTGGGTCTA ACAGACCGGT TGAGCCCAAC 
ACAACACGCT GGTCCGATAG CCACATTCAC AACGGAACCC TCCACGAAGC CACCGAGACG 
CTTTCCTTCC CCAGGCCCAG GGCTGCCACT GTGGTCCCAG AAGCCAGACC TGATATCACC 
TCTGTGACTG TTACTGAGAC GGTCTCACCA GGACGTCCTC ATCCGCCCCC TGCTGTACTT 
CACCCACCCT CGTCCCCCAA GCTTCTCTCA GATGCAAAGG CCAAGCAAGG CCTGCCTGAG 
GTGTCCAATC TTGTGCCTTT GGAGAAGTCT AGTCCAAAGG TGCACTCATC TGTAAACATT 
AGGACTCCTG AGCCATGCAC ACAGGCCCTG GACTCCCCAG GGCTCCAGGA TCTCAGCCAG 
CAAGTGCCTA ACATGGAGAC AGACTGTGCG GGGTTAAGTC ACAGCAGTGA TGAGGACCAC 
CACCCCAGTG CCACATTACA AAGGGCTGAG CTCACGCCCA CGCCTCCCTC CACTCTAAGC 
ATCGATCGCA TTGACTTCTT CAGTGCCAGA GAGAAGTTCC AGGGCCTTTC TCAAGAGATC 
CGCGGGACTG ACCAGGTGGT AGGGCAGGCG CCTCGGGACA AAAGTCCAAG TGTAGAGACC 
AAACGGCTGC TTCCTGACAA CCCCATCAAA GAGGAGGAAC AGAAAAAGGA GAGCTGCAGT 
GTCTCAGTCC ATAGCAAAAT GTCCGGAGTG GAGTCCACAT GGCACAATGT AACCCCTTCC 
CCCCCTCCAA CCAGTCAACC TGTTTCCAGT AGCAGCAGTG AGGAAGAGGA GTTTGCTCAA 
GTCATGTCAG CAGAAGCTGA AATGGAGAAG GTGGAGCATG GGAAAGACTG GCCTAAGGGC 
ACAGTGTGGC GAGCCACCCG GGAGCTGGAG CAAAAGATCA AGCAGGAGGT CTCGTCAGTA 
GCGCTGGTGT CCTCTGCTCC CGTGTGTCCC TCGCGACGCT CCCCCGTCAA CACCAGCAGC 
ATGGAGAAAA CTGAGGAGAC CTCATCCCAC ACACACCTCA CACCTGATCC AAACACTGTG 
TCAACACGCC ATCACCAGCC TCAGTACCAC AAGGTGGAGA AAGACCGGAG TTTTTCATTT 
GAAGAGGGAA GTGAAAGCGA GAGACGACTC AGGGAAGGAG AGCACAGGAA CACCGGCCTC 
ACACACTCGA GACAAAGCCA TTCGCACTCG TCCAGTTGCT CTCCGCCTCT GGTTCTCCAG 
CTCGAGGGCA TCACAGAGCA GGAGAGTTAC ACGGATTCTC GCTGTCCGAA GGACATGAGG 
GAAACCTGGG AGACCCTAAA AGAGCTCGGG GCGTTTCTCT GGCAGGTGAG CAACTGCTCC 
GACAGGAGGC TCAGCCAACC GTGGTCTAGG AGGGAGCAAA GGAAAGGCCG GGCTGCTCGA 
GAGAGGACCA AAGAGGTGGA GGCTCGGATC CGGCAGGCTG GACTGACCCC ACCCTCAAAG 
ATGAAGCGTT CAGCCTCGTT GGCCAAGCTT GGGTCGCTGG AGATCTCGCC TATTGACCTG 
AGCGAACTTG ACTTGAGTCC TGCCTTACTC TCTGCTCCCA CCAATAAGCC TCGGCCCAAT 
TCATACAGTG ACGATACCTC AAAAAAGCAG CGCGTTTTAC CCCACTGCAC TCCTGCTCCC 
CTCCCTTCCT CTTCTGCCTC CCCTGTTTCT TCCACTGGAT CTCCTATGCT TGGACATGCA 
GAAAACGATG ACACGACTGA AGAGGGGGAA TACAGTGACA ATGATGTCAT GCCCATGCCA 
CTGCCACACT CCCCGCCCTC CAATCAGTGT TGGAGGGGGG GAAATGCACA GGAGGAGGCA 
GGATGCGCCC CGTTACCTGC TTCGACCTTG ATACCTGTGG CAACACGCCA ACAATATGGG 
AGGACCCATC CCCTGCGGAG ACTGAAGAAA CGGACCGTTA ACCCTTACCA CACTATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ssh2b ( XM_009295650.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293925.1 Danio rerio slingshot protein phosphatase 2b (ssh2b), transcript variant X1, mRNA.

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CloneID	ODa26174
Clone ID Related Accession (Same CDS sequence)	XM_009295650.2 , XM_009295650.3
Accession Version	XM_009295650.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein phosphatase Slingshot homolog 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009295650.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGTTT CCTCACCGCT GTCTGATGTA AAGACGTTAA GTGGTCTGGA GACTGATTTA 
AACACATCCA CAGAATGTCT GTTGGCTCAC GATGGGATAC AAATACTGCT GCAAAGTGGC 
TTCATGCACC AGGACAACAT CAAACAGTTC ATCAGTGAGA GCTTCCTAAC AGTCAAAGGT 
GCCGCCCTTT TCCTTCCACG TGGGAATGGC TCCTCTGCTG CCTCGTCCTG CTCCTCCAGA 
ATCACAGCAC AGCGCAGCAA ACATGCAGGG GACCTTCAGC AGCACCTGCA GACCATGTTC 
ACTTTACTGC GGCCCGAGGA TAACATCCGC TTGGCTGTGC GGTTGGAGAG CACCTTTCCC 
CAGTGCACCC GCTACATGGT GGTGGTCTCC ACTAATGGCA GGCAGGACAC AGAAGAGAGT 
GTGGTGCTGG GAATGGACTT CAGCCCCTCC GACAGTTCCT GTTCCATAGG CATGGTGCTT 
CCCTTGTGGA GTGACACACG GATCCATCTG GATGGTGACG GGGGCTTCAG TGTGTCTACA 
GATAACAAAA TACACATATT CAAGCCAGTG TCAGTTCAAG CCATGTGGTC TGCCTTGCAG 
TCGCTTCATA AGGCCTGTGA AGTGGCCCGC TGTCATAACT ACTTCCCGGG CAGCCTCTTC 
CTGACCTGGG TGAGTTACTA CCAGAGCCGG GTGTCCTCAG ATCAGGCCCG CATCAACGAG 
TGGAACGCCA TGCAGGACGT GCAGTCCCAC CGTGCAGACT CACCTGTGCT CTTCTCTGAT 
GTGCCAACTG AGCGGGAAAG AACAGAGAGA TTCATCAAGA CTCGATTAAG AGAGATCATG 
ATGCTAAAGG ATCTTGAAAA TGTCACTTCA AAAGATATCC GCACAGAGCT GGAGATGCAG 
ATGGTGTGTA ACCTTCGGGA GTTCAAGGAG TACATCGATA ACGAGATGAT CGTCATCTTG 
GGGCAGATGG ACAGGCCCAC AAAGATTTTT GAGCATGTTT ACCTGGGTTC TGAATGGAAT 
GCATCAAATC TGGAAGAACT GCAAAACACC GGGGTGCAAT ATATCCTTAA TGTGACGCGG 
GAGATCGATA ACTTCTTTCC AGGGCTATTT GAATACCACA ATATCCGGGT TTACGATGAA 
GAAGCCACAG ACTTGCTGGC TTACTGGAAT GACACTTACA AGTTCATTTC TAGGGCCAAA 
AAAGCCGGAG CGAAGTGTCT TGTGCACTGC AAGATGGGTG TTAGTCGTTC GGCCTCCACT 
GTTATCGCAT ACGCCATGAA GGAGTACGGC TGGGACATGG AGCAGGCCTT TGAGTACGTA 
AAAGAGCGGC GAGCCGTCAC CAAACCCAAC CCATCATTCA TGAGACAGCT TGTGGAGTAC 
CAGGGAATAC TGATAGCAAG CAAACAGAGA CACAATAAAT TGTGGCGTTC CCACTCAGAT 
AGTGATCTTT CGGAGCGACT CGAGCCACTC TGTAAAGCTA CTGCCGCACA GTCTCTAGGG 
CGGTCCAGTT CCCGCAATCA GACCTTCGGC CAGACTTCAC CCTCAATACA GGAGCTACTA 
CAGACTCTCA CCCCTTCCTC AGCTACTGCT ACTGGGTCTA ACAGACCGGT TGAGCCCAAC 
ACAACACGCT GGTCCGATAG CCACATTCAC AACGGAACCC TCCACGAAGC CACCGAGACG 
CTTTCCTTCC CCAGGCCCAG GGCTGCCACT GTGGTCCCAG AAGCCAGACC TGATATCACC 
TCTGTGACTG TTACTGAGAC GGTCTCACCA GGACGTCCTC ATCCGCCCCC TGCTGTACTT 
CACCCACCCT CGTCCCCCAA GCTTCTCTCA GATGCAAAGG CCAAGCAAGG CCTGCCTGAG 
GTGTCCAATC TTGTGCCTTT GGAGAAGTCT AGTCCAAAGG TGCACTCATC TGTAAACATT 
AGGACTCCTG AGCCATGCAC ACAGGCCCTG GACTCCCCAG GGCTCCAGGA TCTCAGCCAG 
CAAGTGCCTA ACATGGAGAC AGACTGTGCG GGGTTAAGTC ACAGCAGTGA TGAGGACCAC 
CACCCCAGTG CCACATTACA AAGGGCTGAG CTCACGCCCA CGCCTCCCTC CACTCTAAGC 
ATCGATCGCA TTGACTTCTT CAGTGCCAGA GAGAAGTTCC AGGGCCTTTC TCAAGAGATC 
CGCGGGACTG ACCAGGTGGT AGGGCAGGCG CCTCGGGACA AAAGTCCAAG TGTAGAGACC 
AAACGGCTGC TTCCTGACAA CCCCATCAAA GAGGAGGAAC AGAAAAAGGA GAGCTGCAGT 
GTCTCAGTCC ATAGCAAAAT GTCCGGAGTG GAGTCCACAT GGCACAATGT AACCCCTTCC 
CCCCCTCCAA CCAGTCAACC TGTTTCCAGT AGCAGCAGTG AGGAAGAGGA GTTTGCTCAA 
GTCATGTCAG CAGAAGCTGA AATGGAGAAG GTGGAGCATG GGAAAGACTG GCCTAAGGGC 
ACAGTGTGGC GAGCCACCCG GGAGCTGGAG CAAAAGATCA AGCAGGAGGT CTCGTCAGTA 
GCGCTGGTGT CCTCTGCTCC CGTGTGTCCC TCGCGACGCT CCCCCGTCAA CACCAGCAGC 
ATGGAGAAAA CTGAGGAGAC CTCATCCCAC ACACACCTCA CACCTGATCC AAACACTGTG 
TCAACACGCC ATCACCAGCC TCAGTACCAC AAGGTGGAGA AAGACCGGAG TTTTTCATTT 
GAAGAGGGAA GTGAAAGCGA GAGACGACTC AGGGAAGGAG AGCACAGGAA CACCGGCCTC 
ACACACTCGA GACAAAGCCA TTCGCACTCG TCCAGTTGCT CTCCGCCTCT GGTTCTCCAG 
CTCGAGGGCA TCACAGAGCA GGAGAGTTAC ACGGATTCTC GCTGTCCGAA GGACATGAGG 
GAAACCTGGG AGACCCTAAA AGAGCTCGGG GCGTTTCTCT GGCAGGTGAG CAACTGCTCC 
GACAGGAGGC TCAGCCAACC GTGGTCTAGG AGGGAGCAAA GGAAAGGCCG GGCTGCTCGA 
GAGAGGACCA AAGAGGTGGA GGCTCGGATC CGGCAGGCTG GACTGACCCC ACCCTCAAAG 
ATGAAGCGTT CAGCCTCGTT GGCCAAGCTT GGGTCGCTGG AGATCTCGCC TATTGACCTG 
AGCGAACTTG ACTTGAGTCC TGCCTTACTC TCTGCTCCCA CCAATAAGCC TCGGCCCAAT 
TCATACAGTG ACGATACCTC AAAAAAGCAG CGCGTTTTAC CCCACTGCAC TCCTGCTCCC 
CTCCCTTCCT CTTCTGCCTC CCCTGTTTCT TCCACTGGAT CTCCTATGCT TGGACATGCA 
GAAAACGATG ACACGACTGA AGAGGGGGAA TACAGTGACA ATGATGTCAT GCCCATGCCA 
CTGCCACACT CCCCGCCCTC CAATCAGTGT TGGAGGGGGG GAAATGCACA GGAGGAGGCA 
GGATGCGCCC CGTTACCTGC TTCGACCTTG ATACCTGTGG CAACACGCCA ACAATATGGG 
AGGACCCATC CCCTGCGGAG ACTGAAGAAA CGGACCGTTA ACCCTTACCA CACTATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293925.1
CDS	141..3680
Translation	MGVSSPLSDVKTLSGLETDLNTSTECLLAHDGIQILLQSGFMHQDNIKQFISESFLTVKGAALFLPRGNGSSAASSCSSRITAQRSKHAGDLQQHLQTMFTLLRPEDNIRLAVRLESTFPQCTRYMVVVSTNGRQDTEESVVLGMDFSPSDSSCSIGMVLPLWSDTRIHLDGDGGFSVSTDNKIHIFKPVSVQAMWSALQSLHKACEVARCHNYFPGSLFLTWVSYYQSRVSSDQARINEWNAMQDVQSHRADSPVLFSDVPTERERTERFIKTRLREIMMLKDLENVTSKDIRTELEMQMVCNLREFKEYIDNEMIVILGQMDRPTKIFEHVYLGSEWNASNLEELQNTGVQYILNVTREIDNFFPGLFEYHNIRVYDEEATDLLAYWNDTYKFISRAKKAGAKCLVHCKMGVSRSASTVIAYAMKEYGWDMEQAFEYVKERRAVTKPNPSFMRQLVEYQGILIASKQRHNKLWRSHSDSDLSERLEPLCKATAAQSLGRSSSRNQTFGQTSPSIQELLQTLTPSSATATGSNRPVEPNTTRWSDSHIHNGTLHEATETLSFPRPRAATVVPEARPDITSVTVTETVSPGRPHPPPAVLHPPSSPKLLSDAKAKQGLPEVSNLVPLEKSSPKVHSSVNIRTPEPCTQALDSPGLQDLSQQVPNMETDCAGLSHSSDEDHHPSATLQRAELTPTPPSTLSIDRIDFFSAREKFQGLSQEIRGTDQVVGQAPRDKSPSVETKRLLPDNPIKEEEQKKESCSVSVHSKMSGVESTWHNVTPSPPPTSQPVSSSSSEEEEFAQVMSAEAEMEKVEHGKDWPKGTVWRATRELEQKIKQEVSSVALVSSAPVCPSRRSPVNTSSMEKTEETSSHTHLTPDPNTVSTRHHQPQYHKVEKDRSFSFEEGSESERRLREGEHRNTGLTHSRQSHSHSSSCSPPLVLQLEGITEQESYTDSRCPKDMRETWETLKELGAFLWQVSNCSDRRLSQPWSRREQRKGRAARERTKEVEARIRQAGLTPPSKMKRSASLAKLGSLEISPIDLSELDLSPALLSAPTNKPRPNSYSDDTSKKQRVLPHCTPAPLPSSSASPVSSTGSPMLGHAENDDTTEEGEYSDNDVMPMPLPHSPPSNQCWRGGNAQEEAGCAPLPASTLIPVATRQQYGRTHPLRRLKKRTVNPYHTM

Target ORF information:

RefSeq Version	XM_009295650.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio slingshot protein phosphatase 2b (ssh2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295650.3

ORF Insert Sequence:

ATGGGGGTTT CCTCACCGCT GTCTGATGTA AAGACGTTAA GTGGTCTGGA GACTGATTTA 
AACACATCCA CAGAATGTCT GTTGGCTCAC GATGGGATAC AAATACTGCT GCAAAGTGGC 
TTCATGCACC AGGACAACAT CAAACAGTTC ATCAGTGAGA GCTTCCTAAC AGTCAAAGGT 
GCCGCCCTTT TCCTTCCACG TGGGAATGGC TCCTCTGCTG CCTCGTCCTG CTCCTCCAGA 
ATCACAGCAC AGCGCAGCAA ACATGCAGGG GACCTTCAGC AGCACCTGCA GACCATGTTC 
ACTTTACTGC GGCCCGAGGA TAACATCCGC TTGGCTGTGC GGTTGGAGAG CACCTTTCCC 
CAGTGCACCC GCTACATGGT GGTGGTCTCC ACTAATGGCA GGCAGGACAC AGAAGAGAGT 
GTGGTGCTGG GAATGGACTT CAGCCCCTCC GACAGTTCCT GTTCCATAGG CATGGTGCTT 
CCCTTGTGGA GTGACACACG GATCCATCTG GATGGTGACG GGGGCTTCAG TGTGTCTACA 
GATAACAAAA TACACATATT CAAGCCAGTG TCAGTTCAAG CCATGTGGTC TGCCTTGCAG 
TCGCTTCATA AGGCCTGTGA AGTGGCCCGC TGTCATAACT ACTTCCCGGG CAGCCTCTTC 
CTGACCTGGG TGAGTTACTA CCAGAGCCGG GTGTCCTCAG ATCAGGCCCG CATCAACGAG 
TGGAACGCCA TGCAGGACGT GCAGTCCCAC CGTGCAGACT CACCTGTGCT CTTCTCTGAT 
GTGCCAACTG AGCGGGAAAG AACAGAGAGA TTCATCAAGA CTCGATTAAG AGAGATCATG 
ATGCTAAAGG ATCTTGAAAA TGTCACTTCA AAAGATATCC GCACAGAGCT GGAGATGCAG 
ATGGTGTGTA ACCTTCGGGA GTTCAAGGAG TACATCGATA ACGAGATGAT CGTCATCTTG 
GGGCAGATGG ACAGGCCCAC AAAGATTTTT GAGCATGTTT ACCTGGGTTC TGAATGGAAT 
GCATCAAATC TGGAAGAACT GCAAAACACC GGGGTGCAAT ATATCCTTAA TGTGACGCGG 
GAGATCGATA ACTTCTTTCC AGGGCTATTT GAATACCACA ATATCCGGGT TTACGATGAA 
GAAGCCACAG ACTTGCTGGC TTACTGGAAT GACACTTACA AGTTCATTTC TAGGGCCAAA 
AAAGCCGGAG CGAAGTGTCT TGTGCACTGC AAGATGGGTG TTAGTCGTTC GGCCTCCACT 
GTTATCGCAT ACGCCATGAA GGAGTACGGC TGGGACATGG AGCAGGCCTT TGAGTACGTA 
AAAGAGCGGC GAGCCGTCAC CAAACCCAAC CCATCATTCA TGAGACAGCT TGTGGAGTAC 
CAGGGAATAC TGATAGCAAG CAAACAGAGA CACAATAAAT TGTGGCGTTC CCACTCAGAT 
AGTGATCTTT CGGAGCGACT CGAGCCACTC TGTAAAGCTA CTGCCGCACA GTCTCTAGGG 
CGGTCCAGTT CCCGCAATCA GACCTTCGGC CAGACTTCAC CCTCAATACA GGAGCTACTA 
CAGACTCTCA CCCCTTCCTC AGCTACTGCT ACTGGGTCTA ACAGACCGGT TGAGCCCAAC 
ACAACACGCT GGTCCGATAG CCACATTCAC AACGGAACCC TCCACGAAGC CACCGAGACG 
CTTTCCTTCC CCAGGCCCAG GGCTGCCACT GTGGTCCCAG AAGCCAGACC TGATATCACC 
TCTGTGACTG TTACTGAGAC GGTCTCACCA GGACGTCCTC ATCCGCCCCC TGCTGTACTT 
CACCCACCCT CGTCCCCCAA GCTTCTCTCA GATGCAAAGG CCAAGCAAGG CCTGCCTGAG 
GTGTCCAATC TTGTGCCTTT GGAGAAGTCT AGTCCAAAGG TGCACTCATC TGTAAACATT 
AGGACTCCTG AGCCATGCAC ACAGGCCCTG GACTCCCCAG GGCTCCAGGA TCTCAGCCAG 
CAAGTGCCTA ACATGGAGAC AGACTGTGCG GGGTTAAGTC ACAGCAGTGA TGAGGACCAC 
CACCCCAGTG CCACATTACA AAGGGCTGAG CTCACGCCCA CGCCTCCCTC CACTCTAAGC 
ATCGATCGCA TTGACTTCTT CAGTGCCAGA GAGAAGTTCC AGGGCCTTTC TCAAGAGATC 
CGCGGGACTG ACCAGGTGGT AGGGCAGGCG CCTCGGGACA AAAGTCCAAG TGTAGAGACC 
AAACGGCTGC TTCCTGACAA CCCCATCAAA GAGGAGGAAC AGAAAAAGGA GAGCTGCAGT 
GTCTCAGTCC ATAGCAAAAT GTCCGGAGTG GAGTCCACAT GGCACAATGT AACCCCTTCC 
CCCCCTCCAA CCAGTCAACC TGTTTCCAGT AGCAGCAGTG AGGAAGAGGA GTTTGCTCAA 
GTCATGTCAG CAGAAGCTGA AATGGAGAAG GTGGAGCATG GGAAAGACTG GCCTAAGGGC 
ACAGTGTGGC GAGCCACCCG GGAGCTGGAG CAAAAGATCA AGCAGGAGGT CTCGTCAGTA 
GCGCTGGTGT CCTCTGCTCC CGTGTGTCCC TCGCGACGCT CCCCCGTCAA CACCAGCAGC 
ATGGAGAAAA CTGAGGAGAC CTCATCCCAC ACACACCTCA CACCTGATCC AAACACTGTG 
TCAACACGCC ATCACCAGCC TCAGTACCAC AAGGTGGAGA AAGACCGGAG TTTTTCATTT 
GAAGAGGGAA GTGAAAGCGA GAGACGACTC AGGGAAGGAG AGCACAGGAA CACCGGCCTC 
ACACACTCGA GACAAAGCCA TTCGCACTCG TCCAGTTGCT CTCCGCCTCT GGTTCTCCAG 
CTCGAGGGCA TCACAGAGCA GGAGAGTTAC ACGGATTCTC GCTGTCCGAA GGACATGAGG 
GAAACCTGGG AGACCCTAAA AGAGCTCGGG GCGTTTCTCT GGCAGGTGAG CAACTGCTCC 
GACAGGAGGC TCAGCCAACC GTGGTCTAGG AGGGAGCAAA GGAAAGGCCG GGCTGCTCGA 
GAGAGGACCA AAGAGGTGGA GGCTCGGATC CGGCAGGCTG GACTGACCCC ACCCTCAAAG 
ATGAAGCGTT CAGCCTCGTT GGCCAAGCTT GGGTCGCTGG AGATCTCGCC TATTGACCTG 
AGCGAACTTG ACTTGAGTCC TGCCTTACTC TCTGCTCCCA CCAATAAGCC TCGGCCCAAT 
TCATACAGTG ACGATACCTC AAAAAAGCAG CGCGTTTTAC CCCACTGCAC TCCTGCTCCC 
CTCCCTTCCT CTTCTGCCTC CCCTGTTTCT TCCACTGGAT CTCCTATGCT TGGACATGCA 
GAAAACGATG ACACGACTGA AGAGGGGGAA TACAGTGACA ATGATGTCAT GCCCATGCCA 
CTGCCACACT CCCCGCCCTC CAATCAGTGT TGGAGGGGGG GAAATGCACA GGAGGAGGCA 
GGATGCGCCC CGTTACCTGC TTCGACCTTG ATACCTGTGG CAACACGCCA ACAATATGGG 
AGGACCCATC CCCTGCGGAG ACTGAAGAAA CGGACCGTTA ACCCTTACCA CACTATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Evolution of the tbx6/16 subfamily genes in vertebrates: insights from zebrafish.
Molecular biology and evolution29(12)3959-83(2012 Dec)
Ahn D,You KH,Kim CH

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ssh2b cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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