LOC556872 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC556872
Entrez Gene ID	556872
Full Name	novel protein similar to vertebrate membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1)
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017357508.2	XP_017212997.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X3
XM_021478476.1	XP_021334151.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X4
XM_017357508.1	XP_017212997.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1
XM_021478474.1	XP_021334149.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X1
XM_021478475.1	XP_021334150.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	magixa	XP_001337248.4
Danio rerio (zebrafish)	LOC556872	XP_684886.5

Gene Ontology

The following LOC556872 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC556872 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa143810	XM_021478476.1 Latest version!	Danio rerio novel protein similar to vertebrate membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1) (LOC556872), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa42447	XM_021478474.1 Latest version!	Danio rerio novel protein similar to vertebrate membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1) (LOC556872), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa42448	XM_021478475.1 Latest version!	Danio rerio novel protein similar to vertebrate membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1) (LOC556872), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa60098	XM_017357508.2 Latest version!	Danio rerio novel protein similar to vertebrate membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1) (LOC556872), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
View Old Accession Versions >>
ODa60098	XM_017357508.1	Danio rerio novel protein similar to vertebrate membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1) (LOC556872), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC556872 ( XM_021478476.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334151.1 Danio rerio novel protein similar to vertebrate membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1) (LOC556872), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa143810
Clone ID Related Accession (Same CDS sequence)	XM_021478476.1
Accession Version	XM_021478476.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAAGG TTGCAGTAAA GAAGCTGCAC TGGCGGTCCA AAGTGCAGGA GAACTTCGTC 
CCACTGGTCG GCTCTGGAGA GTTGGGGATA GCCATCGGGG GCGGGGCCGA TTATGGAGAA 
TTCCCATTCG TCACGGCTGC ACCAGGGGGC GGGATCACAG TTGGCGATAT CATCTTAGAA 
ATTGGAGGGA CTCCTGTTTT GGGCCTGACT TTAGGAGATG TGCGTGGACT TCTCAACTCC 
TGCCCTCATC CGATCCGCAT CAAGACTGTT TCTCCAGGCT CAACTCTATG CAAGGATCTG 
AGATTATACT TGAGCAAATG TTTCACACCA GGATCTATGG ACAGTCAGCT CCAGCAGGTG 
ATCCGAGAAA ACCTCTACCT GAGAGCCGTG CCATGTACAA CTAGACAGCC ACGAGACGGT 
GAGATCTCTG GGGTCGACTA TAACTTTGTG TCAGTCGAAG AGTTCTTCTC TCTGGAGGAG 
TCGGGGGCGC TGTTGGAGAG CGGAAAGTTC AAAGAAAAAA AGAACGGCTT CGAATCACTT 
GAGGGAAATT ACTACGGGAC TCCTCGTCCG GTTCACATTG GCCCTGACAG CCCTCCCATC 
AGCTACCAGG AGCACCGCAA CCTGCTAAGA AACTTCCGCA CTCGCAGTAA ATCACTCAGC 
AACCTGGAGA AAGCAGGAGA GGAAGGCGAC AACAGTGAGG AGGACTCAGG TCTATCAGGT 
GGTTCAGCCG GGGCGAGCAG TGTCCCCGTC CACACGTCAT CCCCCAGCCA ATCACGAAAC 
TTAAGAGGTG GAAACACCAG TGCCTTGGAG AATGGGCTCG GACCCCGTAG AGGAGCACGG 
GTGAGAGGGT CGGAAAACTG GGAGATGATG TACAGTGACT CAGGGAATGC CTATTACAAA 
GACCATCATC TAAAGCCATC GTCCTCGTCC TGGCTCAATC GCAGAGCTCA CAGCAGAGAC 
AATGTCTGTA TGCCCGAATA CATTTCACAT TCATGCATTT GGCAGTCACT TACAGACTTA 
CAGTCACAAT GTGTTCAAGT AGCAGAGTTT ACGGACCAGC CGAGTGAACT GAGAGGTTTC 
TCCGTCCACA CTCGTCTAGT TAAAGGCGCA CGGGGCTTCG GTTTCAATAT AGTGGGAGGC 
AGCAGAAGGA GAGAGTTCCT GCAGATCTAC AGCATCACTC CCGGAGGACC TTCAACCCTG 
AACACAGCTG ACATCCTAGT CTACATCAAT GATACCTGTG TGCTCGGCTA CTCTCACAAA 
GATGTAGTAG AAATGCTAAA AGCCATCCCT ATTGGTCATA CTGTGGACAT CATGGTCCGA 
AGAGGTTACC CCATGCTCTA CAACTCAGAA GGTTGTCCTA AGATGTCCAA TCCAAGGCTG 
ACTGCATCCC AAAACCAGTC ACCACATCTA CCTCCACCAA CCACCACCCA ACCGCAGGCC 
CAGGCTCCTC TCCCATATGC ACAGCTCCAC CTCAACCACA ACAGCCCCCC GTGGGCTGAC 
TTTGACAGGG GGTCTTCGAG GAATCTCAGA GTGCGAGCTT CAAGGTTTAG TCTGGATGCT 
AATGGAAATT CACCTCCATT TGCTTCACCT CCAAGACCTC CGTCATCGTA CCAGGGCTCC 
AACGGGTATG GGAGTGGGTT TTTGAGACCG CCACGCACTG CGAGAAGCAT ACGGAGGTTG 
CAGAGCGCAG AACTGGCCAG CCAAAGTGAC AGTGAGGTGG TGTCTGCAAT CGGATCCCAC 
AGGGCCTCGT TTATCCGTAA CCACAACAAC AACTCTCTCT CCACTCCGCC ACCTCTCCGC 
CTGCACTCCT TCAAATCTTC AGAGAGTGAC CTTTCCTCAA GTACTCCACC GCCCATATCT 
CGATTACCTC TACCCCACAC TGAACTAAGC CCAGGTCCTG TCTCCTTTTC CCCTGGAGGT 
GGAAGACGCT ACTCGCATTT ACGTAAACCT CAGAATTCAA TGCTAACCCC ACCAAGCAGC 
GTCCATTCTG ACGGGCCTTA CTTCACCTTC AACGGGACGG CCAGCGCCAG CAGCGGCAGC 
CCAAACAGCA GCATTCCTTC ACCCGGAGCC CTGAGTGCCG TCATAGGCAG TGGTGTTGGA 
GGAATGGCGG CAGGTGGAGA ATTGGTTCCT GTTGCGTTGG CTAAATGCGA GAGGGGCAGC 
AATATGGGGT TCAGTGTGAC GGCGGGAGGG CACGGAGGCA GACAGACCCT TGTGAAGAAA 
GTCTGGGACC ACAGACAGTG TAATGGGCTG CAGTCTGGAG ATGCTGTTGT GAAGATCAAT 
GGAGCTGATG TACAGAGTCT CAGCTTTGCA CAGGTCCAAA GGATTTTGCA AGAACACACT 
AAAAAAGGAG AGGTTATTCT GCTGGTTTAC AGGGGCCCCT CAAACTATTC CAATTCGCCT 
AGTTCTGTGC GAAGACTAAC TCCTCCCCCT CCACGCTCTG CTTCCGACTC TGAAGTTCCT 
TTTACCGACT CCATGCCATT ATCTCGTAAC TCTCTGACCC CGCCATCTCC AGCGCTGGTG 
CGCTCTTCAT TGGTCCAGAG CACCAGCTTT CTGGATTCAG TACCTGTCAC TCTCACACTG 
GAGCCACGTG ATTGGATGAG CTCAGAGGAT GGAGGGACGG TGCTCAGTCC CAGAGTGAAA 
ACAGAGGAGA AAGAGCAGCC CCCGGCGCTG CGGGGCTATG AGGTGGAGCT GAAGAGGAAA 
CCAGGTGAAG GATTTGGATT CGTTATCGCC TCTCAGGATG TGGAAAATGT CAAAGCGGTG 
TCTCTGCTGC CGCACAGGTT TGTGACGGTG CGCAGAGGAA GCCCAGCGGC ACGCAGCGGT 
CAGATCAGGC CTGGCGATCG CCTGGAAGCT GTGGACGGCA GATCAGTGGT CAATTTACCT 
CACAGGGAGC TGGCTCAGAT CCTGAGGAGA GCTGGAAACA CTCTCCGCTT GACCATCGTA 
CCTCGCGCCA GTGCTCATTC CTCAGAGCGA GCAGATTATG ACCCTGATTA TAAGAACAGG 
AAAACTCACA AGTCAAAACC AAAGCAGGAT GCCACGCGGT ACTACAGTGT GGAACTGGAG 
CGGGGTCCCA CTGGATTCGG CTTTAGTCTG AGAGGAGGAA GTGAATACAA CATGGGCCTA 
TATGTGCTGG GACTGATGGA GGGAGGACCG GCCTCACGCA GCCACAAAAT ACAGGTGAGT 
GATCAGTTGG TGGAGATCAA CGGAGACAGT GCAGCAGGAA TGACACACAG TCAGGCAGTG 
GAGCAGATCA GGACTGGAGG AAGCAGGATT CACCTAGTCT TCAAAAGAGG AAACGGATAC 
GTTCCTGACT ACGTGGAGCT CTCCAGTCTC TCTCTCTGTA TGACCAACTC CAAGCTGGGC 
GAACCATGTT TCTATGTGAT TGGAAGGACA GAAAATACGC GACCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334151.1
CDS	413..3820
Translation	MSKVAVKKLHWRSKVQENFVPLVGSGELGIAIGGGADYGEFPFVTAAPGGGITVGDIILEIGGTPVLGLTLGDVRGLLNSCPHPIRIKTVSPGSTLCKDLRLYLSKCFTPGSMDSQLQQVIRENLYLRAVPCTTRQPRDGEISGVDYNFVSVEEFFSLEESGALLESGKFKEKKNGFESLEGNYYGTPRPVHIGPDSPPISYQEHRNLLRNFRTRSKSLSNLEKAGEEGDNSEEDSGLSGGSAGASSVPVHTSSPSQSRNLRGGNTSALENGLGPRRGARVRGSENWEMMYSDSGNAYYKDHHLKPSSSSWLNRRAHSRDNVCMPEYISHSCIWQSLTDLQSQCVQVAEFTDQPSELRGFSVHTRLVKGARGFGFNIVGGSRRREFLQIYSITPGGPSTLNTADILVYINDTCVLGYSHKDVVEMLKAIPIGHTVDIMVRRGYPMLYNSEGCPKMSNPRLTASQNQSPHLPPPTTTQPQAQAPLPYAQLHLNHNSPPWADFDRGSSRNLRVRASRFSLDANGNSPPFASPPRPPSSYQGSNGYGSGFLRPPRTARSIRRLQSAELASQSDSEVVSAIGSHRASFIRNHNNNSLSTPPPLRLHSFKSSESDLSSSTPPPISRLPLPHTELSPGPVSFSPGGGRRYSHLRKPQNSMLTPPSSVHSDGPYFTFNGTASASSGSPNSSIPSPGALSAVIGSGVGGMAAGGELVPVALAKCERGSNMGFSVTAGGHGGRQTLVKKVWDHRQCNGLQSGDAVVKINGADVQSLSFAQVQRILQEHTKKGEVILLVYRGPSNYSNSPSSVRRLTPPPPRSASDSEVPFTDSMPLSRNSLTPPSPALVRSSLVQSTSFLDSVPVTLTLEPRDWMSSEDGGTVLSPRVKTEEKEQPPALRGYEVELKRKPGEGFGFVIASQDVENVKAVSLLPHRFVTVRRGSPAARSGQIRPGDRLEAVDGRSVVNLPHRELAQILRRAGNTLRLTIVPRASAHSSERADYDPDYKNRKTHKSKPKQDATRYYSVELERGPTGFGFSLRGGSEYNMGLYVLGLMEGGPASRSHKIQVSDQLVEINGDSAAGMTHSQAVEQIRTGGSRIHLVFKRGNGYVPDYVELSSLSLCMTNSKLGEPCFYVIGRTENTRP

Target ORF information:

RefSeq Version	XM_021478476.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio novel protein similar to vertebrate membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1) (LOC556872), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478476.1

ORF Insert Sequence:

ATGTCTAAGG TTGCAGTAAA GAAGCTGCAC TGGCGGTCCA AAGTGCAGGA GAACTTCGTC 
CCACTGGTCG GCTCTGGAGA GTTGGGGATA GCCATCGGGG GCGGGGCCGA TTATGGAGAA 
TTCCCATTCG TCACGGCTGC ACCAGGGGGC GGGATCACAG TTGGCGATAT CATCTTAGAA 
ATTGGAGGGA CTCCTGTTTT GGGCCTGACT TTAGGAGATG TGCGTGGACT TCTCAACTCC 
TGCCCTCATC CGATCCGCAT CAAGACTGTT TCTCCAGGCT CAACTCTATG CAAGGATCTG 
AGATTATACT TGAGCAAATG TTTCACACCA GGATCTATGG ACAGTCAGCT CCAGCAGGTG 
ATCCGAGAAA ACCTCTACCT GAGAGCCGTG CCATGTACAA CTAGACAGCC ACGAGACGGT 
GAGATCTCTG GGGTCGACTA TAACTTTGTG TCAGTCGAAG AGTTCTTCTC TCTGGAGGAG 
TCGGGGGCGC TGTTGGAGAG CGGAAAGTTC AAAGAAAAAA AGAACGGCTT CGAATCACTT 
GAGGGAAATT ACTACGGGAC TCCTCGTCCG GTTCACATTG GCCCTGACAG CCCTCCCATC 
AGCTACCAGG AGCACCGCAA CCTGCTAAGA AACTTCCGCA CTCGCAGTAA ATCACTCAGC 
AACCTGGAGA AAGCAGGAGA GGAAGGCGAC AACAGTGAGG AGGACTCAGG TCTATCAGGT 
GGTTCAGCCG GGGCGAGCAG TGTCCCCGTC CACACGTCAT CCCCCAGCCA ATCACGAAAC 
TTAAGAGGTG GAAACACCAG TGCCTTGGAG AATGGGCTCG GACCCCGTAG AGGAGCACGG 
GTGAGAGGGT CGGAAAACTG GGAGATGATG TACAGTGACT CAGGGAATGC CTATTACAAA 
GACCATCATC TAAAGCCATC GTCCTCGTCC TGGCTCAATC GCAGAGCTCA CAGCAGAGAC 
AATGTCTGTA TGCCCGAATA CATTTCACAT TCATGCATTT GGCAGTCACT TACAGACTTA 
CAGTCACAAT GTGTTCAAGT AGCAGAGTTT ACGGACCAGC CGAGTGAACT GAGAGGTTTC 
TCCGTCCACA CTCGTCTAGT TAAAGGCGCA CGGGGCTTCG GTTTCAATAT AGTGGGAGGC 
AGCAGAAGGA GAGAGTTCCT GCAGATCTAC AGCATCACTC CCGGAGGACC TTCAACCCTG 
AACACAGCTG ACATCCTAGT CTACATCAAT GATACCTGTG TGCTCGGCTA CTCTCACAAA 
GATGTAGTAG AAATGCTAAA AGCCATCCCT ATTGGTCATA CTGTGGACAT CATGGTCCGA 
AGAGGTTACC CCATGCTCTA CAACTCAGAA GGTTGTCCTA AGATGTCCAA TCCAAGGCTG 
ACTGCATCCC AAAACCAGTC ACCACATCTA CCTCCACCAA CCACCACCCA ACCGCAGGCC 
CAGGCTCCTC TCCCATATGC ACAGCTCCAC CTCAACCACA ACAGCCCCCC GTGGGCTGAC 
TTTGACAGGG GGTCTTCGAG GAATCTCAGA GTGCGAGCTT CAAGGTTTAG TCTGGATGCT 
AATGGAAATT CACCTCCATT TGCTTCACCT CCAAGACCTC CGTCATCGTA CCAGGGCTCC 
AACGGGTATG GGAGTGGGTT TTTGAGACCG CCACGCACTG CGAGAAGCAT ACGGAGGTTG 
CAGAGCGCAG AACTGGCCAG CCAAAGTGAC AGTGAGGTGG TGTCTGCAAT CGGATCCCAC 
AGGGCCTCGT TTATCCGTAA CCACAACAAC AACTCTCTCT CCACTCCGCC ACCTCTCCGC 
CTGCACTCCT TCAAATCTTC AGAGAGTGAC CTTTCCTCAA GTACTCCACC GCCCATATCT 
CGATTACCTC TACCCCACAC TGAACTAAGC CCAGGTCCTG TCTCCTTTTC CCCTGGAGGT 
GGAAGACGCT ACTCGCATTT ACGTAAACCT CAGAATTCAA TGCTAACCCC ACCAAGCAGC 
GTCCATTCTG ACGGGCCTTA CTTCACCTTC AACGGGACGG CCAGCGCCAG CAGCGGCAGC 
CCAAACAGCA GCATTCCTTC ACCCGGAGCC CTGAGTGCCG TCATAGGCAG TGGTGTTGGA 
GGAATGGCGG CAGGTGGAGA ATTGGTTCCT GTTGCGTTGG CTAAATGCGA GAGGGGCAGC 
AATATGGGGT TCAGTGTGAC GGCGGGAGGG CACGGAGGCA GACAGACCCT TGTGAAGAAA 
GTCTGGGACC ACAGACAGTG TAATGGGCTG CAGTCTGGAG ATGCTGTTGT GAAGATCAAT 
GGAGCTGATG TACAGAGTCT CAGCTTTGCA CAGGTCCAAA GGATTTTGCA AGAACACACT 
AAAAAAGGAG AGGTTATTCT GCTGGTTTAC AGGGGCCCCT CAAACTATTC CAATTCGCCT 
AGTTCTGTGC GAAGACTAAC TCCTCCCCCT CCACGCTCTG CTTCCGACTC TGAAGTTCCT 
TTTACCGACT CCATGCCATT ATCTCGTAAC TCTCTGACCC CGCCATCTCC AGCGCTGGTG 
CGCTCTTCAT TGGTCCAGAG CACCAGCTTT CTGGATTCAG TACCTGTCAC TCTCACACTG 
GAGCCACGTG ATTGGATGAG CTCAGAGGAT GGAGGGACGG TGCTCAGTCC CAGAGTGAAA 
ACAGAGGAGA AAGAGCAGCC CCCGGCGCTG CGGGGCTATG AGGTGGAGCT GAAGAGGAAA 
CCAGGTGAAG GATTTGGATT CGTTATCGCC TCTCAGGATG TGGAAAATGT CAAAGCGGTG 
TCTCTGCTGC CGCACAGGTT TGTGACGGTG CGCAGAGGAA GCCCAGCGGC ACGCAGCGGT 
CAGATCAGGC CTGGCGATCG CCTGGAAGCT GTGGACGGCA GATCAGTGGT CAATTTACCT 
CACAGGGAGC TGGCTCAGAT CCTGAGGAGA GCTGGAAACA CTCTCCGCTT GACCATCGTA 
CCTCGCGCCA GTGCTCATTC CTCAGAGCGA GCAGATTATG ACCCTGATTA TAAGAACAGG 
AAAACTCACA AGTCAAAACC AAAGCAGGAT GCCACGCGGT ACTACAGTGT GGAACTGGAG 
CGGGGTCCCA CTGGATTCGG CTTTAGTCTG AGAGGAGGAA GTGAATACAA CATGGGCCTA 
TATGTGCTGG GACTGATGGA GGGAGGACCG GCCTCACGCA GCCACAAAAT ACAGGTGAGT 
GATCAGTTGG TGGAGATCAA CGGAGACAGT GCAGCAGGAA TGACACACAG TCAGGCAGTG 
GAGCAGATCA GGACTGGAGG AAGCAGGATT CACCTAGTCT TCAAAAGAGG AAACGGATAC 
GTTCCTGACT ACGTGGAGCT CTCCAGTCTC TCTCTCTGTA TGACCAACTC CAAGCTGGGC 
GAACCATGTT TCTATGTGAT TGGAAGGACA GAAAATACGC GACCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC556872 ( XM_021478474.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334149.1 Danio rerio novel protein similar to vertebrate membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1) (LOC556872), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa42447
Clone ID Related Accession (Same CDS sequence)	XM_021478474.1
Accession Version	XM_021478474.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAAGG TTGCAGTAAA GAAGCTGCAC TGGCGGTCCA AAGTGCAGGA GAACTTCGTC 
CCACTGGTCG GCTCTGGAGA GTTGGGGATA GCCATCGGGG GCGGGGCCGA TTATGGAGAA 
TTCCCATTCG TCACGGCTGC ACCAGGGGGC GGGATCACAG TTGGCGATAT CATCTTAGAA 
ATTGGAGGGA CTCCTGTTTT GGGCCTGACT TTAGGAGATG TGCGTGGACT TCTCAACTCC 
TGCCCTCATC CGATCCGCAT CAAGACTGTT TCTCCAGGCT CAACTCTATG CAAGGATCTG 
AGATTATACT TGAGCAAATG TTTCACACCA GGATCTATGG ACAGTCAGCT CCAGCAGGTG 
ATCCGAGAAA ACCTCTACCT GAGAGCCGTG CCATGTACAA CTAGACAGCC ACGAGACGGT 
GAGATCTCTG GGGTCGACTA TAACTTTGTG TCAGTCGAAG AGTTCTTCTC TCTGGAGGAG 
TCGGGGGCGC TGTTGGAGAG CGGAAAGTTC AAAGAAAAAA AGAACGGCTT CGAATCACTT 
GAGGGAAATT ACTACGGGAC TCCTCGTCCG GTTCACATTG GCCCTGACAG CCCTCCCATC 
AGCTACCAGG AGCACCGCAA CCTGCTAAGA AACTTCCGCA CTCGCAGTAA ATCACTCAGC 
AACCTGGAGA AAGCAGGAGA GGAAGGCGAC AACAGTGAGG AGGACTCAGG TCTATCAGGT 
GGTTCAGCCG GGGCGAGCAG TGTCCCCGTC CACACGTCAT CCCCCAGCCA ATCACGAAAC 
TTAAGAGGTG GAAACACCAG TGCCTTGGAG AATGGGCTCG GACCCCGTAG AGGAGCACGG 
GTGAGAGGGT CGGAAAACTG GGAGATGATG TACAGTGACT CAGGGAATGC CTATTACAAA 
GACCATCATC TAAAGCCATC GTCCTCGTCC TGGCTCAATC GCAGAGCTCA CAGCAGAGAC 
AATGTCTGTA TGCCCGAATA CATTTCACAT TCATGCATTT GGCAGTCACT TACAGACTTA 
CAGTCACAAT GTGTTCAAGT AGCAGAGTTT ACGGACCAGC CGAGTGAACT GAGAGGTTTC 
TCCGTCCACA CTCGTCTAGT TAAAGGCGCA CGGGGCTTCG GTTTCAATAT AGTGGGAGGC 
AGCAGAAGGA GAGAGTTCCT GCAGATCTAC AGCATCACTC CCGGAGGACC TTCAACCCTG 
AACACAGCTG ACATCCTAGT CTACATCAAT GATACCTGTG TGCTCGGCTA CTCTCACAAA 
GATGTAGTAG AAATGCTAAA AGCCATCCCT ATTGGTCATA CTGTGGACAT CATGGTCCGA 
AGAGGTTACC CCATGCTCTA CAACTCAGAA GGTTGTCCTA AGATGTCCAA TCCAAGGCTG 
ACTGCATCCC AAAACCAGTC ACCACATCTA CCTCCACCAA CCACCACCCA ACCGCAGGCC 
CAGGCTCCTC TCCCATATGC ACAGCTCCAC CTCAACCACA ACAGCCCCCC GTGGGCTGAC 
TTTGACAGGG GGTCTTCGAG GAATCTCAGA GTGCGAGCTT CAAGGTTTAG TCTGGATGCT 
AATGGAAATT CACCTCCATT TGCTTCACCT CCAAGACCTC CGTCATCGTA CCAGGGCTCC 
AACGGGTATG GGAGTGGGTT TTTGAGACCG CCACGCACTG CGAGAAGCAT ACGGAGGTTG 
CAGAGCGCAG AACTGGCCAG CCAAAGTGAC AGTGAGGTGG TGTCTGCAAT CGGATCCCAC 
AGGGCCTCGT TTATCCGTAA CCACAACAAC AACTCTCTCT CCACTCCGCC ACCTCTCCGC 
CTGCACTCCT TCAAATCTTC AGAGAGTGAC CTTTCCTCAA GTACTCCACC GCCCATATCT 
CGATTACCTC TACCCCACAC TGAACTAAGC CCAGGTCCTG TCTCCTTTTC CCCTGGAGGT 
GGAAGACGCT ACTCGCATTT ACGTAAACCT CAGAATTCAA TGCTAACCCC ACCAAGCAGC 
GTCCATTCTG ACGGGCCTTA CTTCACCTTC AACGGGACGG CCAGCGCCAG CAGCGGCAGC 
CCAAACAGCA GCATTCCTTC ACCCGGAGCC CTGAGTGCCG TCATAGGCAG TGGTGTTGGA 
GGAATGGCGG CAGGTGGAGA ATTGGTTCCT GTTGCGTTGG CTAAATGCGA GAGGGGCAGC 
AATATGGGGT TCAGTGTGAC GGCGGGAGGG CACGGAGGCA GACAGACCCT TGTGAAGAAA 
GTCTGGGACC ACAGACAGTG TAATGGGCTG CAGTCTGGAG ATGCTGTTGT GAAGATCAAT 
GGAGCTGATG TACAGAGTCT CAGCTTTGCA CAGGTCCAAA GGATTTTGCA AGAACACACT 
AAAAAAGGAG AGGTTATTCT GCTGGTTTAC AGGGGCCCCT CAAACTATTC CAATTCGCCT 
AGTTCTGTGC GAAGACTAAC TCCTCCCCCT CCACGCTCTG CTTCCGACTC TGAAGTTCCT 
TTTACCGACT CCATGCCATT ATCTCGTAAC TCTCTGACCC CGCCATCTCC AGCGCTGGTG 
CGCTCTTCAT TGGTCCAGAG CACCAGCTTT CTGGATTCAG TACCTGTCAC TCTCACACTG 
GAGCCACGTG ATTGGATGAG CTCAGAGGAT GGAGGGACGG TGCTCAGTCC CAGAGTGAAA 
ACAGAGGAGA AAGAGCAGCC CCCGGCGCTG CGGGGCTATG AGGTGGAGCT GAAGAGGAAA 
CCAGGTGAAG GATTTGGATT CGTTATCGCC TCTCAGGATG TGGAAAATGT CAAAGCGGTG 
TCTCTGCTGC CGCACAGGTT TGTGACGGTG CGCAGAGGAA GCCCAGCGGC ACGCAGCGGT 
CAGATCAGGC CTGGCGATCG CCTGGAAGCT GTGGACGGCA GATCAGTGGT CAATTTACCT 
CACAGGGAGC TGGCTCAGAT CCTGAGGAGA GCTGGAAACA CTCTCCGCTT GACCATCGTA 
CCTCGCGCCA GTGCTCATTC CTCAGAGCGA GCAGATTATG ACCCTGATTA TAAGAACAGG 
AAAACTCACA AGTCAAAACC AAAGCAGGAT GCCACGCGGT ACTACAGTGT GGAACTGGAG 
CGGGGTCCCA CTGGATTCGG CTTTAGTCTG AGAGGAGGAA GTGAATACAA CATGGGCCTA 
TATGTGCTGG GACTGATGGA GGGAGGACCG GCCTCACGCA GCCACAAAAT ACAGGTGAGT 
GATCAGTTGG TGGAGATCAA CGGAGACAGT GCAGCAGGAA TGACACACAG TCAGGCAGTG 
GAGCAGATCA GGACTGGAGG AAGCAGGATT CACCTAGTCT TCAAAAGAGG AAACGGATAC 
GTTCCTGACT ACGACCGTGA TCACATCACA GCCGTCTCCT CCTCACCCCA GCCCCTTCAG 
TCGAATTTGG CTGCAGTGAG CCACCACCGG CCTCAACGGG ACGCCACATC CAGCCTTCAA 
AAACCAGAGA AGACCAACCG AGGGTGGAAA GAACCGAGGG AGAGGAGTGA CGGAGGAGAC 
CGCGAGAGCC CTGCAAGACT CAAGGGTGAC CGTCAGGGCA GAAGGCGAAG GTCAAGAGAT 
GATGAAAGGA GACGAAGGTC ATACAGCTTG CCTAGAAACA CACCAAGAAG ACACAGCGAT 
GAAAGCATGA AGGTTGAAAA GGTTGAAAGA AATGGGAGAG AGATGGAAAA AGGTAACAAG 
AGGAGCGTGG ACAGGCCAAA ACGTAGCAAG AGTGAGGAGA GGGTGAGAGA AAGAAAGAGA 
GAAGGAGATG AGAGTAAGAA CACACAAACC ATCAATAAAC ACAGACTTCC TCAGCCCAAA 
CTGCAGTGGG AGGAAAATGA GGAGGAGGAG GAGGAGTGGG ATGGAGAGAC TGAGCAAGAG 
TGGACAAGAG AGAGAGAAAG GCTAAAAGAC AAAAAACGGG AGAGGGAGGA GATAGCGTCT 
CTTGATTCAG AGAGCGACGG GGAATTAGAA AGCGAAAATG AGAGTAAGAT GAATGTGGAA 
GTGCTTGAGT GGGAGACGAA CAAAAAAACC CATCAAGACA TTTCTGAGGA ATTGAACAGA 
GAGCAAGATA CAAGTTTCCA TAAGCGCTTG CCTTATTTGG GCATTCGTGT ACTTCCTCCA 
GACACTCAGT CCCGCCCTCT TAGACGCATT TCTGGCCACT CATTGGAGGA GCCCGTAGGG 
AAGAATGAAT TCAATAGGAC GCCGTTTGCT TTCCTTACAA CTGAGCAGTC TGATTACGCA 
GAGTCGGAGT CAGGAGCCAG TGTGTCTGAG TGTAGCATAT CGGCAGCCAG TATCTCTGGT 
CTATCAGTGT ATATGACAGA AAGGTGTCAT TTAAATAGCA AACACAATTC CCAGGAGCCC 
TTACTTCCAG GCCTGTGGTT AAAACCGAGC TTGGAGAGAC TCATTAGAGA GGGTAAGGAG 
ATCAGGATGA TGGGGAAAGG TGGAGGAACA GAATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334149.1
CDS	409..4827
Translation	MSKVAVKKLHWRSKVQENFVPLVGSGELGIAIGGGADYGEFPFVTAAPGGGITVGDIILEIGGTPVLGLTLGDVRGLLNSCPHPIRIKTVSPGSTLCKDLRLYLSKCFTPGSMDSQLQQVIRENLYLRAVPCTTRQPRDGEISGVDYNFVSVEEFFSLEESGALLESGKFKEKKNGFESLEGNYYGTPRPVHIGPDSPPISYQEHRNLLRNFRTRSKSLSNLEKAGEEGDNSEEDSGLSGGSAGASSVPVHTSSPSQSRNLRGGNTSALENGLGPRRGARVRGSENWEMMYSDSGNAYYKDHHLKPSSSSWLNRRAHSRDNVCMPEYISHSCIWQSLTDLQSQCVQVAEFTDQPSELRGFSVHTRLVKGARGFGFNIVGGSRRREFLQIYSITPGGPSTLNTADILVYINDTCVLGYSHKDVVEMLKAIPIGHTVDIMVRRGYPMLYNSEGCPKMSNPRLTASQNQSPHLPPPTTTQPQAQAPLPYAQLHLNHNSPPWADFDRGSSRNLRVRASRFSLDANGNSPPFASPPRPPSSYQGSNGYGSGFLRPPRTARSIRRLQSAELASQSDSEVVSAIGSHRASFIRNHNNNSLSTPPPLRLHSFKSSESDLSSSTPPPISRLPLPHTELSPGPVSFSPGGGRRYSHLRKPQNSMLTPPSSVHSDGPYFTFNGTASASSGSPNSSIPSPGALSAVIGSGVGGMAAGGELVPVALAKCERGSNMGFSVTAGGHGGRQTLVKKVWDHRQCNGLQSGDAVVKINGADVQSLSFAQVQRILQEHTKKGEVILLVYRGPSNYSNSPSSVRRLTPPPPRSASDSEVPFTDSMPLSRNSLTPPSPALVRSSLVQSTSFLDSVPVTLTLEPRDWMSSEDGGTVLSPRVKTEEKEQPPALRGYEVELKRKPGEGFGFVIASQDVENVKAVSLLPHRFVTVRRGSPAARSGQIRPGDRLEAVDGRSVVNLPHRELAQILRRAGNTLRLTIVPRASAHSSERADYDPDYKNRKTHKSKPKQDATRYYSVELERGPTGFGFSLRGGSEYNMGLYVLGLMEGGPASRSHKIQVSDQLVEINGDSAAGMTHSQAVEQIRTGGSRIHLVFKRGNGYVPDYDRDHITAVSSSPQPLQSNLAAVSHHRPQRDATSSLQKPEKTNRGWKEPRERSDGGDRESPARLKGDRQGRRRRSRDDERRRRSYSLPRNTPRRHSDESMKVEKVERNGREMEKGNKRSVDRPKRSKSEERVRERKREGDESKNTQTINKHRLPQPKLQWEENEEEEEEWDGETEQEWTRERERLKDKKREREEIASLDSESDGELESENESKMNVEVLEWETNKKTHQDISEELNREQDTSFHKRLPYLGIRVLPPDTQSRPLRRISGHSLEEPVGKNEFNRTPFAFLTTEQSDYAESESGASVSECSISAASISGLSVYMTERCHLNSKHNSQEPLLPGLWLKPSLERLIREGKEIRMMGKGGGTEC

Target ORF information:

RefSeq Version	XM_021478474.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio novel protein similar to vertebrate membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1) (LOC556872), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478474.1

ORF Insert Sequence:

ATGTCTAAGG TTGCAGTAAA GAAGCTGCAC TGGCGGTCCA AAGTGCAGGA GAACTTCGTC 
CCACTGGTCG GCTCTGGAGA GTTGGGGATA GCCATCGGGG GCGGGGCCGA TTATGGAGAA 
TTCCCATTCG TCACGGCTGC ACCAGGGGGC GGGATCACAG TTGGCGATAT CATCTTAGAA 
ATTGGAGGGA CTCCTGTTTT GGGCCTGACT TTAGGAGATG TGCGTGGACT TCTCAACTCC 
TGCCCTCATC CGATCCGCAT CAAGACTGTT TCTCCAGGCT CAACTCTATG CAAGGATCTG 
AGATTATACT TGAGCAAATG TTTCACACCA GGATCTATGG ACAGTCAGCT CCAGCAGGTG 
ATCCGAGAAA ACCTCTACCT GAGAGCCGTG CCATGTACAA CTAGACAGCC ACGAGACGGT 
GAGATCTCTG GGGTCGACTA TAACTTTGTG TCAGTCGAAG AGTTCTTCTC TCTGGAGGAG 
TCGGGGGCGC TGTTGGAGAG CGGAAAGTTC AAAGAAAAAA AGAACGGCTT CGAATCACTT 
GAGGGAAATT ACTACGGGAC TCCTCGTCCG GTTCACATTG GCCCTGACAG CCCTCCCATC 
AGCTACCAGG AGCACCGCAA CCTGCTAAGA AACTTCCGCA CTCGCAGTAA ATCACTCAGC 
AACCTGGAGA AAGCAGGAGA GGAAGGCGAC AACAGTGAGG AGGACTCAGG TCTATCAGGT 
GGTTCAGCCG GGGCGAGCAG TGTCCCCGTC CACACGTCAT CCCCCAGCCA ATCACGAAAC 
TTAAGAGGTG GAAACACCAG TGCCTTGGAG AATGGGCTCG GACCCCGTAG AGGAGCACGG 
GTGAGAGGGT CGGAAAACTG GGAGATGATG TACAGTGACT CAGGGAATGC CTATTACAAA 
GACCATCATC TAAAGCCATC GTCCTCGTCC TGGCTCAATC GCAGAGCTCA CAGCAGAGAC 
AATGTCTGTA TGCCCGAATA CATTTCACAT TCATGCATTT GGCAGTCACT TACAGACTTA 
CAGTCACAAT GTGTTCAAGT AGCAGAGTTT ACGGACCAGC CGAGTGAACT GAGAGGTTTC 
TCCGTCCACA CTCGTCTAGT TAAAGGCGCA CGGGGCTTCG GTTTCAATAT AGTGGGAGGC 
AGCAGAAGGA GAGAGTTCCT GCAGATCTAC AGCATCACTC CCGGAGGACC TTCAACCCTG 
AACACAGCTG ACATCCTAGT CTACATCAAT GATACCTGTG TGCTCGGCTA CTCTCACAAA 
GATGTAGTAG AAATGCTAAA AGCCATCCCT ATTGGTCATA CTGTGGACAT CATGGTCCGA 
AGAGGTTACC CCATGCTCTA CAACTCAGAA GGTTGTCCTA AGATGTCCAA TCCAAGGCTG 
ACTGCATCCC AAAACCAGTC ACCACATCTA CCTCCACCAA CCACCACCCA ACCGCAGGCC 
CAGGCTCCTC TCCCATATGC ACAGCTCCAC CTCAACCACA ACAGCCCCCC GTGGGCTGAC 
TTTGACAGGG GGTCTTCGAG GAATCTCAGA GTGCGAGCTT CAAGGTTTAG TCTGGATGCT 
AATGGAAATT CACCTCCATT TGCTTCACCT CCAAGACCTC CGTCATCGTA CCAGGGCTCC 
AACGGGTATG GGAGTGGGTT TTTGAGACCG CCACGCACTG CGAGAAGCAT ACGGAGGTTG 
CAGAGCGCAG AACTGGCCAG CCAAAGTGAC AGTGAGGTGG TGTCTGCAAT CGGATCCCAC 
AGGGCCTCGT TTATCCGTAA CCACAACAAC AACTCTCTCT CCACTCCGCC ACCTCTCCGC 
CTGCACTCCT TCAAATCTTC AGAGAGTGAC CTTTCCTCAA GTACTCCACC GCCCATATCT 
CGATTACCTC TACCCCACAC TGAACTAAGC CCAGGTCCTG TCTCCTTTTC CCCTGGAGGT 
GGAAGACGCT ACTCGCATTT ACGTAAACCT CAGAATTCAA TGCTAACCCC ACCAAGCAGC 
GTCCATTCTG ACGGGCCTTA CTTCACCTTC AACGGGACGG CCAGCGCCAG CAGCGGCAGC 
CCAAACAGCA GCATTCCTTC ACCCGGAGCC CTGAGTGCCG TCATAGGCAG TGGTGTTGGA 
GGAATGGCGG CAGGTGGAGA ATTGGTTCCT GTTGCGTTGG CTAAATGCGA GAGGGGCAGC 
AATATGGGGT TCAGTGTGAC GGCGGGAGGG CACGGAGGCA GACAGACCCT TGTGAAGAAA 
GTCTGGGACC ACAGACAGTG TAATGGGCTG CAGTCTGGAG ATGCTGTTGT GAAGATCAAT 
GGAGCTGATG TACAGAGTCT CAGCTTTGCA CAGGTCCAAA GGATTTTGCA AGAACACACT 
AAAAAAGGAG AGGTTATTCT GCTGGTTTAC AGGGGCCCCT CAAACTATTC CAATTCGCCT 
AGTTCTGTGC GAAGACTAAC TCCTCCCCCT CCACGCTCTG CTTCCGACTC TGAAGTTCCT 
TTTACCGACT CCATGCCATT ATCTCGTAAC TCTCTGACCC CGCCATCTCC AGCGCTGGTG 
CGCTCTTCAT TGGTCCAGAG CACCAGCTTT CTGGATTCAG TACCTGTCAC TCTCACACTG 
GAGCCACGTG ATTGGATGAG CTCAGAGGAT GGAGGGACGG TGCTCAGTCC CAGAGTGAAA 
ACAGAGGAGA AAGAGCAGCC CCCGGCGCTG CGGGGCTATG AGGTGGAGCT GAAGAGGAAA 
CCAGGTGAAG GATTTGGATT CGTTATCGCC TCTCAGGATG TGGAAAATGT CAAAGCGGTG 
TCTCTGCTGC CGCACAGGTT TGTGACGGTG CGCAGAGGAA GCCCAGCGGC ACGCAGCGGT 
CAGATCAGGC CTGGCGATCG CCTGGAAGCT GTGGACGGCA GATCAGTGGT CAATTTACCT 
CACAGGGAGC TGGCTCAGAT CCTGAGGAGA GCTGGAAACA CTCTCCGCTT GACCATCGTA 
CCTCGCGCCA GTGCTCATTC CTCAGAGCGA GCAGATTATG ACCCTGATTA TAAGAACAGG 
AAAACTCACA AGTCAAAACC AAAGCAGGAT GCCACGCGGT ACTACAGTGT GGAACTGGAG 
CGGGGTCCCA CTGGATTCGG CTTTAGTCTG AGAGGAGGAA GTGAATACAA CATGGGCCTA 
TATGTGCTGG GACTGATGGA GGGAGGACCG GCCTCACGCA GCCACAAAAT ACAGGTGAGT 
GATCAGTTGG TGGAGATCAA CGGAGACAGT GCAGCAGGAA TGACACACAG TCAGGCAGTG 
GAGCAGATCA GGACTGGAGG AAGCAGGATT CACCTAGTCT TCAAAAGAGG AAACGGATAC 
GTTCCTGACT ACGACCGTGA TCACATCACA GCCGTCTCCT CCTCACCCCA GCCCCTTCAG 
TCGAATTTGG CTGCAGTGAG CCACCACCGG CCTCAACGGG ACGCCACATC CAGCCTTCAA 
AAACCAGAGA AGACCAACCG AGGGTGGAAA GAACCGAGGG AGAGGAGTGA CGGAGGAGAC 
CGCGAGAGCC CTGCAAGACT CAAGGGTGAC CGTCAGGGCA GAAGGCGAAG GTCAAGAGAT 
GATGAAAGGA GACGAAGGTC ATACAGCTTG CCTAGAAACA CACCAAGAAG ACACAGCGAT 
GAAAGCATGA AGGTTGAAAA GGTTGAAAGA AATGGGAGAG AGATGGAAAA AGGTAACAAG 
AGGAGCGTGG ACAGGCCAAA ACGTAGCAAG AGTGAGGAGA GGGTGAGAGA AAGAAAGAGA 
GAAGGAGATG AGAGTAAGAA CACACAAACC ATCAATAAAC ACAGACTTCC TCAGCCCAAA 
CTGCAGTGGG AGGAAAATGA GGAGGAGGAG GAGGAGTGGG ATGGAGAGAC TGAGCAAGAG 
TGGACAAGAG AGAGAGAAAG GCTAAAAGAC AAAAAACGGG AGAGGGAGGA GATAGCGTCT 
CTTGATTCAG AGAGCGACGG GGAATTAGAA AGCGAAAATG AGAGTAAGAT GAATGTGGAA 
GTGCTTGAGT GGGAGACGAA CAAAAAAACC CATCAAGACA TTTCTGAGGA ATTGAACAGA 
GAGCAAGATA CAAGTTTCCA TAAGCGCTTG CCTTATTTGG GCATTCGTGT ACTTCCTCCA 
GACACTCAGT CCCGCCCTCT TAGACGCATT TCTGGCCACT CATTGGAGGA GCCCGTAGGG 
AAGAATGAAT TCAATAGGAC GCCGTTTGCT TTCCTTACAA CTGAGCAGTC TGATTACGCA 
GAGTCGGAGT CAGGAGCCAG TGTGTCTGAG TGTAGCATAT CGGCAGCCAG TATCTCTGGT 
CTATCAGTGT ATATGACAGA AAGGTGTCAT TTAAATAGCA AACACAATTC CCAGGAGCCC 
TTACTTCCAG GCCTGTGGTT AAAACCGAGC TTGGAGAGAC TCATTAGAGA GGGTAAGGAG 
ATCAGGATGA TGGGGAAAGG TGGAGGAACA GAATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC556872 ( XM_021478475.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334150.1 Danio rerio novel protein similar to vertebrate membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1) (LOC556872), transcript variant X2, mRNA.

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CloneID	ODa42448
Clone ID Related Accession (Same CDS sequence)	XM_021478475.1
Accession Version	XM_021478475.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAAGG TTGCAGTAAA GAAGCTGCAC TGGCGGTCCA AAGTGCAGGA GAACTTCGTC 
CCACTGGTCG GCTCTGGAGA GTTGGGGATA GCCATCGGGG GCGGGGCCGA TTATGGAGAA 
TTCCCATTCG TCACGGCTGC ACCAGGGGGC GGGATCACAG TTGGCGATAT CATCTTAGAA 
ATTGGAGGGA CTCCTGTTTT GGGCCTGACT TTAGGAGATG TGCGTGGACT TCTCAACTCC 
TGCCCTCATC CGATCCGCAT CAAGACTGTT TCTCCAGGCT CAACTCTATG CAAGGATCTG 
AGATTATACT TGAGCAAATG TTTCACACCA GGATCTATGG ACAGTCAGCT CCAGCAGGTG 
ATCCGAGAAA ACCTCTACCT GAGAGCCGTG CCATGTACAA CTAGACAGCC ACGAGACGGT 
GAGATCTCTG GGGTCGACTA TAACTTTGTG TCAGTCGAAG AGTTCTTCTC TCTGGAGGAG 
TCGGGGGCGC TGTTGGAGAG CGGAAAGTTC AAAGAAAAAA AGAACGGCTT CGAATCACTT 
GAGGGAAATT ACTACGGGAC TCCTCGTCCG GTTCACATTG GCCCTGACAG CCCTCCCATC 
AGCTACCAGG AGCACCGCAA CCTGCTAAGA AACTTCCGCA CTCGCAGTAA ATCACTCAGC 
AACCTGGAGA AAGCAGGAGA GGAAGGCGAC AACAGTGAGG AGGACTCAGG TCTATCAGGT 
GGTTCAGCCG GGGCGAGCAG TGTCCCCGTC CACACGTCAT CCCCCAGCCA ATCACGAAAC 
TTAAGAGGTG GAAACACCAG TGCCTTGGAG AATGGGCTCG GACCCCGTAG AGGAGCACGG 
GTGAGAGGGT CGGAAAACTG GGAGATGATG TACAGTGACT CAGGGAATGC CTATTACAAA 
GACCATCATC TAAAGCCATC GTCCTCGTCC TGGCTCAATC GCAGAGCTCA CAGCAGAGAC 
AATGTCTGTA TGCCCGAATA CATTTCACAT TCATGCATTT GGCAGTCACT TACAGACTTA 
CAGTCACAAT GTGTTCAAGT AGCAGAGTTT ACGGACCAGC CGAGTGAACT GAGAGGTTTC 
TCCGTCCACA CTCGTCTAGT TAAAGGCGCA CGGGGCTTCG GTTTCAATAT AGTGGGAGGC 
AGCAGAAGGA GAGAGTTCCT GCAGATCTAC AGCATCACTC CCGGAGGACC TTCAACCCTG 
AACACAGCTG ACATCCTAGT CTACATCAAT GATACCTGTG TGCTCGGCTA CTCTCACAAA 
GATGTAGTAG AAATGCTAAA AGCCATCCCT ATTGGTCATA CTGTGGACAT CATGGTCCGA 
AGAGGTTACC CCATGCTCTA CAACTCAGAA GGTTGTCCTA AGATGTCCAA TCCAAGGCTG 
ACTGCATCCC AAAACCAGTC ACCACATCTA CCTCCACCAA CCACCACCCA ACCGCAGGCC 
CAGGCTCCTC TCCCATATGC ACAGCTCCAC CTCAACCACA ACAGCCCCCC GTGGGCTGAC 
TTTGACAGGG GGTCTTCGAG GAATCTCAGA GTGCGAGCTT CAAGGTTTAG TCTGGATGCT 
AATGGAAATT CACCTCCATT TGCTTCACCT CCAAGACCTC CGTCATCGTA CCAGGGCTCC 
AACGGGTATG GGAGTGGGTT TTTGAGACCG CCACGCACTG CGAGAAGCAT ACGGAGGTTG 
CAGAGCGCAG AACTGGCCAG CCAAAGTGAC AGTGAGGTGG TGTCTGCAAT CGGATCCCAC 
AGGGCCTCGT TTATCCGTAA CCACAACAAC AACTCTCTCT CCACTCCGCC ACCTCTCCGC 
CTGCACTCCT TCAAATCTTC AGAGAGTGAC CTTTCCTCAA GTACTCCACC GCCCATATCT 
CGATTACCTC TACCCCACAC TGAACTAAGC CCAGGTCCTG TCTCCTTTTC CCCTGGAGGT 
GGAAGACGCT ACTCGCATTT ACGTAAACCT CAGAATTCAA TGCTAACCCC ACCAAGCAGC 
GTCCATTCTG ACGGGCCTTA CTTCACCTTC AACGGGACGG CCAGCGCCAG CAGCGGCAGC 
CCAAACAGCA GCATTCCTTC ACCCGGAGCC CTGAGTGCCG TCATAGGCAG TGGTGTTGGA 
GGAATGGCGG CAGGTGGAGA ATTGGTTCCT GTTGCGTTGG CTAAATGCGA GAGGGGCAGC 
AATATGGGGT TCAGTGTGAC GGCGGGAGGG CACGGAGGCA GACAGACCCT TGTGAAGAAA 
GTCTGGGACC ACAGACAGTG TAATGGGCTG CAGTCTGGAG ATGCTGTTGT GAAGATCAAT 
GGAGCTGATG TACAGAGTCT CAGCTTTGCA CAGGTCCAAA GGATTTTGCA AGAACACACT 
AAAAAAGGAG AGGTTATTCT GCTGGTTTAC AGGGGCCCCT CAAACTATTC CAATTCGCCT 
AGTTCTGTGC GAAGACTAAC TCCTCCCCCT CCACGCTCTG CTTCCGACTC TGAAGTTCCT 
TTTACCGACT CCATGCCATT ATCTCGTAAC TCTCTGACCC CGCCATCTCC AGCGCTGGTG 
CGCTCTTCAT TGGTCCAGAG CACCAGCTTT CTGGATTCAG TACCTGTCAC TCTCACACTG 
GAGCCACGTG ATTGGATGAG CTCAGAGGAT GGAGGGACGG TGCTCAGTCC CAGAGTGAAA 
ACAGAGGAGA AAGAGCAGCC CCCGGCGCTG CGGGGCTATG AGGTGGAGCT GAAGAGGAAA 
CCAGGTGAAG GATTTGGATT CGTTATCGCC TCTCAGGATG TGGAAAATGT CAAAGCGGTG 
TCTCTGCTGC CGCACAGGTT TGTGACGGTG CGCAGAGGAA GCCCAGCGGC ACGCAGCGGT 
CAGATCAGGC CTGGCGATCG CCTGGAAGCT GTGGACGGCA GATCAGTGGT CAATTTACCT 
CACAGGGAGC TGGCTCAGAT CCTGAGGAGA GCTGGAAACA CTCTCCGCTT GACCATCGTA 
CCTCGCGCCA GTGCTCATTC CTCAGAGCGA GCAGATTATG ACCCTGATTA TAAGAACAGG 
AAAACTCACA AGTCAAAACC AAAGGATGCC ACGCGGTACT ACAGTGTGGA ACTGGAGCGG 
GGTCCCACTG GATTCGGCTT TAGTCTGAGA GGAGGAAGTG AATACAACAT GGGCCTATAT 
GTGCTGGGAC TGATGGAGGG AGGACCGGCC TCACGCAGCC ACAAAATACA GGTGAGTGAT 
CAGTTGGTGG AGATCAACGG AGACAGTGCA GCAGGAATGA CACACAGTCA GGCAGTGGAG 
CAGATCAGGA CTGGAGGAAG CAGGATTCAC CTAGTCTTCA AAAGAGGAAA CGGATACGTT 
CCTGACTACG ACCGTGATCA CATCACAGCC GTCTCCTCCT CACCCCAGCC CCTTCAGTCG 
AATTTGGCTG CAGTGAGCCA CCACCGGCCT CAACGGGACG CCACATCCAG CCTTCAAAAA 
CCAGAGAAGA CCAACCGAGG GTGGAAAGAA CCGAGGGAGA GGAGTGACGG AGGAGACCGC 
GAGAGCCCTG CAAGACTCAA GGGTGACCGT CAGGGCAGAA GGCGAAGGTC AAGAGATGAT 
GAAAGGAGAC GAAGGTCATA CAGCTTGCCT AGAAACACAC CAAGAAGACA CAGCGATGAA 
AGCATGAAGG TTGAAAAGGT TGAAAGAAAT GGGAGAGAGA TGGAAAAAGG TAACAAGAGG 
AGCGTGGACA GGCCAAAACG TAGCAAGAGT GAGGAGAGGG TGAGAGAAAG AAAGAGAGAA 
GGAGATGAGA GTAAGAACAC ACAAACCATC AATAAACACA GACTTCCTCA GCCCAAACTG 
CAGTGGGAGG AAAATGAGGA GGAGGAGGAG GAGTGGGATG GAGAGACTGA GCAAGAGTGG 
ACAAGAGAGA GAGAAAGGCT AAAAGACAAA AAACGGGAGA GGGAGGAGAT AGCGTCTCTT 
GATTCAGAGA GCGACGGGGA ATTAGAAAGC GAAAATGAGA GTAAGATGAA TGTGGAAGTG 
CTTGAGTGGG AGACGAACAA AAAAACCCAT CAAGACATTT CTGAGGAATT GAACAGAGAG 
CAAGATACAA GTTTCCATAA GCGCTTGCCT TATTTGGGCA TTCGTGTACT TCCTCCAGAC 
ACTCAGTCCC GCCCTCTTAG ACGCATTTCT GGCCACTCAT TGGAGGAGCC CGTAGGGAAG 
AATGAATTCA ATAGGACGCC GTTTGCTTTC CTTACAACTG AGCAGTCTGA TTACGCAGAG 
TCGGAGTCAG GAGCCAGTGT GTCTGAGTGT AGCATATCGG CAGCCAGTAT CTCTGGTCTA 
TCAGTGTATA TGACAGAAAG GTGTCATTTA AATAGCAAAC ACAATTCCCA GGAGCCCTTA 
CTTCCAGGCC TGTGGTTAAA ACCGAGCTTG GAGAGACTCA TTAGAGAGGG TAAGGAGATC 
AGGATGATGG GGAAAGGTGG AGGAACAGAA TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334150.1
CDS	409..4824
Translation	MSKVAVKKLHWRSKVQENFVPLVGSGELGIAIGGGADYGEFPFVTAAPGGGITVGDIILEIGGTPVLGLTLGDVRGLLNSCPHPIRIKTVSPGSTLCKDLRLYLSKCFTPGSMDSQLQQVIRENLYLRAVPCTTRQPRDGEISGVDYNFVSVEEFFSLEESGALLESGKFKEKKNGFESLEGNYYGTPRPVHIGPDSPPISYQEHRNLLRNFRTRSKSLSNLEKAGEEGDNSEEDSGLSGGSAGASSVPVHTSSPSQSRNLRGGNTSALENGLGPRRGARVRGSENWEMMYSDSGNAYYKDHHLKPSSSSWLNRRAHSRDNVCMPEYISHSCIWQSLTDLQSQCVQVAEFTDQPSELRGFSVHTRLVKGARGFGFNIVGGSRRREFLQIYSITPGGPSTLNTADILVYINDTCVLGYSHKDVVEMLKAIPIGHTVDIMVRRGYPMLYNSEGCPKMSNPRLTASQNQSPHLPPPTTTQPQAQAPLPYAQLHLNHNSPPWADFDRGSSRNLRVRASRFSLDANGNSPPFASPPRPPSSYQGSNGYGSGFLRPPRTARSIRRLQSAELASQSDSEVVSAIGSHRASFIRNHNNNSLSTPPPLRLHSFKSSESDLSSSTPPPISRLPLPHTELSPGPVSFSPGGGRRYSHLRKPQNSMLTPPSSVHSDGPYFTFNGTASASSGSPNSSIPSPGALSAVIGSGVGGMAAGGELVPVALAKCERGSNMGFSVTAGGHGGRQTLVKKVWDHRQCNGLQSGDAVVKINGADVQSLSFAQVQRILQEHTKKGEVILLVYRGPSNYSNSPSSVRRLTPPPPRSASDSEVPFTDSMPLSRNSLTPPSPALVRSSLVQSTSFLDSVPVTLTLEPRDWMSSEDGGTVLSPRVKTEEKEQPPALRGYEVELKRKPGEGFGFVIASQDVENVKAVSLLPHRFVTVRRGSPAARSGQIRPGDRLEAVDGRSVVNLPHRELAQILRRAGNTLRLTIVPRASAHSSERADYDPDYKNRKTHKSKPKDATRYYSVELERGPTGFGFSLRGGSEYNMGLYVLGLMEGGPASRSHKIQVSDQLVEINGDSAAGMTHSQAVEQIRTGGSRIHLVFKRGNGYVPDYDRDHITAVSSSPQPLQSNLAAVSHHRPQRDATSSLQKPEKTNRGWKEPRERSDGGDRESPARLKGDRQGRRRRSRDDERRRRSYSLPRNTPRRHSDESMKVEKVERNGREMEKGNKRSVDRPKRSKSEERVRERKREGDESKNTQTINKHRLPQPKLQWEENEEEEEEWDGETEQEWTRERERLKDKKREREEIASLDSESDGELESENESKMNVEVLEWETNKKTHQDISEELNREQDTSFHKRLPYLGIRVLPPDTQSRPLRRISGHSLEEPVGKNEFNRTPFAFLTTEQSDYAESESGASVSECSISAASISGLSVYMTERCHLNSKHNSQEPLLPGLWLKPSLERLIREGKEIRMMGKGGGTEC

Target ORF information:

RefSeq Version	XM_021478475.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio novel protein similar to vertebrate membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1) (LOC556872), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478475.1

ORF Insert Sequence:

ATGTCTAAGG TTGCAGTAAA GAAGCTGCAC TGGCGGTCCA AAGTGCAGGA GAACTTCGTC 
CCACTGGTCG GCTCTGGAGA GTTGGGGATA GCCATCGGGG GCGGGGCCGA TTATGGAGAA 
TTCCCATTCG TCACGGCTGC ACCAGGGGGC GGGATCACAG TTGGCGATAT CATCTTAGAA 
ATTGGAGGGA CTCCTGTTTT GGGCCTGACT TTAGGAGATG TGCGTGGACT TCTCAACTCC 
TGCCCTCATC CGATCCGCAT CAAGACTGTT TCTCCAGGCT CAACTCTATG CAAGGATCTG 
AGATTATACT TGAGCAAATG TTTCACACCA GGATCTATGG ACAGTCAGCT CCAGCAGGTG 
ATCCGAGAAA ACCTCTACCT GAGAGCCGTG CCATGTACAA CTAGACAGCC ACGAGACGGT 
GAGATCTCTG GGGTCGACTA TAACTTTGTG TCAGTCGAAG AGTTCTTCTC TCTGGAGGAG 
TCGGGGGCGC TGTTGGAGAG CGGAAAGTTC AAAGAAAAAA AGAACGGCTT CGAATCACTT 
GAGGGAAATT ACTACGGGAC TCCTCGTCCG GTTCACATTG GCCCTGACAG CCCTCCCATC 
AGCTACCAGG AGCACCGCAA CCTGCTAAGA AACTTCCGCA CTCGCAGTAA ATCACTCAGC 
AACCTGGAGA AAGCAGGAGA GGAAGGCGAC AACAGTGAGG AGGACTCAGG TCTATCAGGT 
GGTTCAGCCG GGGCGAGCAG TGTCCCCGTC CACACGTCAT CCCCCAGCCA ATCACGAAAC 
TTAAGAGGTG GAAACACCAG TGCCTTGGAG AATGGGCTCG GACCCCGTAG AGGAGCACGG 
GTGAGAGGGT CGGAAAACTG GGAGATGATG TACAGTGACT CAGGGAATGC CTATTACAAA 
GACCATCATC TAAAGCCATC GTCCTCGTCC TGGCTCAATC GCAGAGCTCA CAGCAGAGAC 
AATGTCTGTA TGCCCGAATA CATTTCACAT TCATGCATTT GGCAGTCACT TACAGACTTA 
CAGTCACAAT GTGTTCAAGT AGCAGAGTTT ACGGACCAGC CGAGTGAACT GAGAGGTTTC 
TCCGTCCACA CTCGTCTAGT TAAAGGCGCA CGGGGCTTCG GTTTCAATAT AGTGGGAGGC 
AGCAGAAGGA GAGAGTTCCT GCAGATCTAC AGCATCACTC CCGGAGGACC TTCAACCCTG 
AACACAGCTG ACATCCTAGT CTACATCAAT GATACCTGTG TGCTCGGCTA CTCTCACAAA 
GATGTAGTAG AAATGCTAAA AGCCATCCCT ATTGGTCATA CTGTGGACAT CATGGTCCGA 
AGAGGTTACC CCATGCTCTA CAACTCAGAA GGTTGTCCTA AGATGTCCAA TCCAAGGCTG 
ACTGCATCCC AAAACCAGTC ACCACATCTA CCTCCACCAA CCACCACCCA ACCGCAGGCC 
CAGGCTCCTC TCCCATATGC ACAGCTCCAC CTCAACCACA ACAGCCCCCC GTGGGCTGAC 
TTTGACAGGG GGTCTTCGAG GAATCTCAGA GTGCGAGCTT CAAGGTTTAG TCTGGATGCT 
AATGGAAATT CACCTCCATT TGCTTCACCT CCAAGACCTC CGTCATCGTA CCAGGGCTCC 
AACGGGTATG GGAGTGGGTT TTTGAGACCG CCACGCACTG CGAGAAGCAT ACGGAGGTTG 
CAGAGCGCAG AACTGGCCAG CCAAAGTGAC AGTGAGGTGG TGTCTGCAAT CGGATCCCAC 
AGGGCCTCGT TTATCCGTAA CCACAACAAC AACTCTCTCT CCACTCCGCC ACCTCTCCGC 
CTGCACTCCT TCAAATCTTC AGAGAGTGAC CTTTCCTCAA GTACTCCACC GCCCATATCT 
CGATTACCTC TACCCCACAC TGAACTAAGC CCAGGTCCTG TCTCCTTTTC CCCTGGAGGT 
GGAAGACGCT ACTCGCATTT ACGTAAACCT CAGAATTCAA TGCTAACCCC ACCAAGCAGC 
GTCCATTCTG ACGGGCCTTA CTTCACCTTC AACGGGACGG CCAGCGCCAG CAGCGGCAGC 
CCAAACAGCA GCATTCCTTC ACCCGGAGCC CTGAGTGCCG TCATAGGCAG TGGTGTTGGA 
GGAATGGCGG CAGGTGGAGA ATTGGTTCCT GTTGCGTTGG CTAAATGCGA GAGGGGCAGC 
AATATGGGGT TCAGTGTGAC GGCGGGAGGG CACGGAGGCA GACAGACCCT TGTGAAGAAA 
GTCTGGGACC ACAGACAGTG TAATGGGCTG CAGTCTGGAG ATGCTGTTGT GAAGATCAAT 
GGAGCTGATG TACAGAGTCT CAGCTTTGCA CAGGTCCAAA GGATTTTGCA AGAACACACT 
AAAAAAGGAG AGGTTATTCT GCTGGTTTAC AGGGGCCCCT CAAACTATTC CAATTCGCCT 
AGTTCTGTGC GAAGACTAAC TCCTCCCCCT CCACGCTCTG CTTCCGACTC TGAAGTTCCT 
TTTACCGACT CCATGCCATT ATCTCGTAAC TCTCTGACCC CGCCATCTCC AGCGCTGGTG 
CGCTCTTCAT TGGTCCAGAG CACCAGCTTT CTGGATTCAG TACCTGTCAC TCTCACACTG 
GAGCCACGTG ATTGGATGAG CTCAGAGGAT GGAGGGACGG TGCTCAGTCC CAGAGTGAAA 
ACAGAGGAGA AAGAGCAGCC CCCGGCGCTG CGGGGCTATG AGGTGGAGCT GAAGAGGAAA 
CCAGGTGAAG GATTTGGATT CGTTATCGCC TCTCAGGATG TGGAAAATGT CAAAGCGGTG 
TCTCTGCTGC CGCACAGGTT TGTGACGGTG CGCAGAGGAA GCCCAGCGGC ACGCAGCGGT 
CAGATCAGGC CTGGCGATCG CCTGGAAGCT GTGGACGGCA GATCAGTGGT CAATTTACCT 
CACAGGGAGC TGGCTCAGAT CCTGAGGAGA GCTGGAAACA CTCTCCGCTT GACCATCGTA 
CCTCGCGCCA GTGCTCATTC CTCAGAGCGA GCAGATTATG ACCCTGATTA TAAGAACAGG 
AAAACTCACA AGTCAAAACC AAAGGATGCC ACGCGGTACT ACAGTGTGGA ACTGGAGCGG 
GGTCCCACTG GATTCGGCTT TAGTCTGAGA GGAGGAAGTG AATACAACAT GGGCCTATAT 
GTGCTGGGAC TGATGGAGGG AGGACCGGCC TCACGCAGCC ACAAAATACA GGTGAGTGAT 
CAGTTGGTGG AGATCAACGG AGACAGTGCA GCAGGAATGA CACACAGTCA GGCAGTGGAG 
CAGATCAGGA CTGGAGGAAG CAGGATTCAC CTAGTCTTCA AAAGAGGAAA CGGATACGTT 
CCTGACTACG ACCGTGATCA CATCACAGCC GTCTCCTCCT CACCCCAGCC CCTTCAGTCG 
AATTTGGCTG CAGTGAGCCA CCACCGGCCT CAACGGGACG CCACATCCAG CCTTCAAAAA 
CCAGAGAAGA CCAACCGAGG GTGGAAAGAA CCGAGGGAGA GGAGTGACGG AGGAGACCGC 
GAGAGCCCTG CAAGACTCAA GGGTGACCGT CAGGGCAGAA GGCGAAGGTC AAGAGATGAT 
GAAAGGAGAC GAAGGTCATA CAGCTTGCCT AGAAACACAC CAAGAAGACA CAGCGATGAA 
AGCATGAAGG TTGAAAAGGT TGAAAGAAAT GGGAGAGAGA TGGAAAAAGG TAACAAGAGG 
AGCGTGGACA GGCCAAAACG TAGCAAGAGT GAGGAGAGGG TGAGAGAAAG AAAGAGAGAA 
GGAGATGAGA GTAAGAACAC ACAAACCATC AATAAACACA GACTTCCTCA GCCCAAACTG 
CAGTGGGAGG AAAATGAGGA GGAGGAGGAG GAGTGGGATG GAGAGACTGA GCAAGAGTGG 
ACAAGAGAGA GAGAAAGGCT AAAAGACAAA AAACGGGAGA GGGAGGAGAT AGCGTCTCTT 
GATTCAGAGA GCGACGGGGA ATTAGAAAGC GAAAATGAGA GTAAGATGAA TGTGGAAGTG 
CTTGAGTGGG AGACGAACAA AAAAACCCAT CAAGACATTT CTGAGGAATT GAACAGAGAG 
CAAGATACAA GTTTCCATAA GCGCTTGCCT TATTTGGGCA TTCGTGTACT TCCTCCAGAC 
ACTCAGTCCC GCCCTCTTAG ACGCATTTCT GGCCACTCAT TGGAGGAGCC CGTAGGGAAG 
AATGAATTCA ATAGGACGCC GTTTGCTTTC CTTACAACTG AGCAGTCTGA TTACGCAGAG 
TCGGAGTCAG GAGCCAGTGT GTCTGAGTGT AGCATATCGG CAGCCAGTAT CTCTGGTCTA 
TCAGTGTATA TGACAGAAAG GTGTCATTTA AATAGCAAAC ACAATTCCCA GGAGCCCTTA 
CTTCCAGGCC TGTGGTTAAA ACCGAGCTTG GAGAGACTCA TTAGAGAGGG TAAGGAGATC 
AGGATGATGG GGAAAGGTGG AGGAACAGAA TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC556872 ( XM_017357508.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212997.1 Danio rerio novel protein similar to vertebrate membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1) (LOC556872), transcript variant X3, mRNA.

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CloneID	ODa60098
Clone ID Related Accession (Same CDS sequence)	XM_017357508.2 , XM_017357508.1
Accession Version	XM_017357508.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017357508.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAAGG TTGCAGTAAA GAAGCTGCAC TGGCGGTCCA AAGTGCAGGA GAACTTCGTC 
CCACTGGTCG GCTCTGGAGA GTTGGGGATA GCCATCGGGG GCGGGGCCGA TTATGGAGAA 
TTCCCATTCG TCACGGCTGC ACCAGGGGGC GGGATCACAG TTGGCGATAT CATCTTAGAA 
ATTGGAGGGA CTCCTGTTTT GGGCCTGACT TTAGGAGATG TGCGTGGACT TCTCAACTCC 
TGCCCTCATC CGATCCGCAT CAAGACTGTT TCTCCAGGCT CAACTCTATG CAAGGATCTG 
AGATTATACT TGAGCAAATG TTTCACACCA GGATCTATGG ACAGTCAGCT CCAGCAGGTG 
ATCCGAGAAA ACCTCTACCT GAGAGCCGTG CCATGTACAA CTAGACAGCC ACGAGACGGT 
GAGATCTCTG GGGTCGACTA TAACTTTGTG TCAGTCGAAG AGTTCTTCTC TCTGGAGGAG 
TCGGGGGCGC TGTTGGAGAG CGGAAAGTTC AAAGAAAAAA AGAACGGCTT CGAATCACTT 
GAGGGAAATT ACTACGGGAC TCCTCGTCCG GTTCACATTG GCCCTGACAG CCCTCCCATC 
AGCTACCAGG AGCACCGCAA CCTGCTAAGA AACTTCCGCA CTCGCAGTAA ATCACTCAGC 
AACCTGGAGA AAGCAGGAGA GGAAGGCGAC AACAGTGAGG AGGACTCAGG TCTATCAGGT 
GGTTCAGCCG GGGCGAGCAG TGTCCCCGTC CACACGTCAT CCCCCAGCCA ATCACGAAAC 
TTAAGAGGTG GAAACACCAG TGCCTTGGAG AATGGGCTCG GACCCCGTAG AGGAGCACGG 
GTGAGAGGGT CGGAAAACTG GGAGATGATG TACAGTGACT CAGGGAATGC CTATTACAAA 
GACCATCATC TAAAGCCATC GTCCTCGTCC TGGCTCAATC GCAGAGCTCA CAGCAGAGAC 
AATGTCTGTA TGCCCGAATT AGCAGAGTTT ACGGACCAGC CGAGTGAACT GAGAGGTTTC 
TCCGTCCACA CTCGTCTAGT TAAAGGCGCA CGGGGCTTCG GTTTCAATAT AGTGGGAGGC 
AGCAGAAGGA GAGAGTTCCT GCAGATCTAC AGCATCACTC CCGGAGGACC TTCAACCCTG 
AACACAGCTG ACATCCTAGT CTACATCAAT GATACCTGTG TGCTCGGCTA CTCTCACAAA 
GATGTAGTAG AAATGCTAAA AGCCATCCCT ATTGGTCATA CTGTGGACAT CATGGTCCGA 
AGAGGTTACC CCATGCTCTA CAACTCAGAA GGTTGTCCTA AGATGTCCAA TCCAAGGCTG 
ACTGCATCCC AAAACCAGTC ACCACATCTA CCTCCACCAA CCACCACCCA ACCGCAGGCC 
CAGGCTCCTC TCCCATATGC ACAGCTCCAC CTCAACCACA ACAGCCCCCC GTGGGCTGAC 
TTTGACAGGG GGTCTTCGAG GAATCTCAGA GTGCGAGCTT CAAGGTTTAG TCTGGATGCT 
AATGGAAATT CACCTCCATT TGCTTCACCT CCAAGACCTC CGTCATCGTA CCAGGGCTCC 
AACGGGTATG GGAGTGGGTT TTTGAGACCG CCACGCACTG CGAGAAGCAT ACGGAGGTTG 
CAGAGCGCAG AACTGGCCAG CCAAAGTGAC AGTGAGGTGG TGTCTGCAAT CGGATCCCAC 
AGGGCCTCGT TTATCCGTAA CCACAACAAC AACTCTCTCT CCACTCCGCC ACCTCTCCGC 
CTGCACTCCT TCAAATCTTC AGAGAGTGAC CTTTCCTCAA GTACTCCACC GCCCATATCT 
CGATTACCTC TACCCCACAC TGAACTAAGC CCAGGTCCTG TCTCCTTTTC CCCTGGAGGT 
GGAAGACGCT ACTCGCATTT ACGTAAACCT CAGAATTCAA TGCTAACCCC ACCAAGCAGC 
GTCCATTCTG ACGGGCCTTA CTTCACCTTC AACGGGACGG CCAGCGCCAG CAGCGGCAGC 
CCAAACAGCA GCATTCCTTC ACCCGGAGCC CTGAGTGCCG TCATAGGCAG TGGTGTTGGA 
GGAATGGCGG CAGGTGGAGA ATTGGTTCCT GTTGCGTTGG CTAAATGCGA GAGGGGCAGC 
AATATGGGGT TCAGTGTGAC GGCGGGAGGG CACGGAGGCA GACAGACCCT TGTGAAGAAA 
GTCTGGGACC ACAGACAGTG TAATGGGCTG CAGTCTGGAG ATGCTGTTGT GAAGATCAAT 
GGAGCTGATG TACAGAGTCT CAGCTTTGCA CAGGTCCAAA GGATTTTGCA AGAACACACT 
AAAAAAGGAG AGGTTATTCT GCTGGTTTAC AGGGGCCCCT CAAACTATTC CAATTCGCCT 
AGTTCTGTGC GAAGACTAAC TCCTCCCCCT CCACGCTCTG CTTCCGACTC TGAAGTTCCT 
TTTACCGACT CCATGCCATT ATCTCGTAAC TCTCTGACCC CGCCATCTCC AGCGCTGGTG 
CGCTCTTCAT TGGTCCAGAG CACCAGCTTT CTGGATTCAG TACCTGTCAC TCTCACACTG 
GAGCCACGTG ATTGGATGAG CTCAGAGGAT GGAGGGACGG TGCTCAGTCC CAGAGTGAAA 
ACAGAGGAGA AAGAGCAGCC CCCGGCGCTG CGGGGCTATG AGGTGGAGCT GAAGAGGAAA 
CCAGGTGAAG GATTTGGATT CGTTATCGCC TCTCAGGATG TGGAAAATGT CAAAGCGGTG 
TCTCTGCTGC CGCACAGGTT TGTGACGGTG CGCAGAGGAA GCCCAGCGGC ACGCAGCGGT 
CAGATCAGGC CTGGCGATCG CCTGGAAGCT GTGGACGGCA GATCAGTGGT CAATTTACCT 
CACAGGGAGC TGGCTCAGAT CCTGAGGAGA GCTGGAAACA CTCTCCGCTT GACCATCGTA 
CCTCGCGCCA GTGCTCATTC CTCAGAGCGA GCAGATTATG ACCCTGATTA TAAGAACAGG 
AAAACTCACA AGTCAAAACC AAAGCAGGAT GCCACGCGGT ACTACAGTGT GGAACTGGAG 
CGGGGTCCCA CTGGATTCGG CTTTAGTCTG AGAGGAGGAA GTGAATACAA CATGGGCCTA 
TATGTGCTGG GACTGATGGA GGGAGGACCG GCCTCACGCA GCCACAAAAT ACAGGTGAGT 
GATCAGTTGG TGGAGATCAA CGGAGACAGT GCAGCAGGAA TGACACACAG TCAGGCAGTG 
GAGCAGATCA GGACTGGAGG AAGCAGGATT CACCTAGTCT TCAAAAGAGG AAACGGATAC 
GTTCCTGACT ACGACCGTGA TCACATCACA GCCGTCTCCT CCTCACCCCA GCCCCTTCAG 
TCGAATTTGG CTGCAGTGAG CCACCACCGG CCTCAACGGG ACGCCACATC CAGCCTTCAA 
AAACCAGAGA AGACCAACCG AGGGTGGAAA GAACCGAGGG AGAGGAGTGA CGGAGGAGAC 
CGCGAGAGCC CTGCAAGACT CAAGGGTGAC CGTCAGGGCA GAAGGCGAAG GTCAAGAGAT 
GATGAAAGGA GACGAAGGTC ATACAGCTTG CCTAGAAACA CACCAAGAAG ACACAGCGAT 
GAAAGCATGA AGGTTGAAAA GGTTGAAAGA AATGGGAGAG AGATGGAAAA AGGTAACAAG 
AGGAGCGTGG ACAGGCCAAA ACGTAGCAAG AGTGAGGAGA GGGTGAGAGA AAGAAAGAGA 
GAAGGAGATG AGAGTAAGAA CACACAAACC ATCAATAAAC ACAGACTTCC TCAGCCCAAA 
CTGCAGTGGG AGGAAAATGA GGAGGAGGAG GAGGAGTGGG ATGGAGAGAC TGAGCAAGAG 
TGGACAAGAG AGAGAGAAAG GCTAAAAGAC AAAAAACGGG AGAGGGAGGA GATAGCGTCT 
CTTGATTCAG AGAGCGACGG GGAATTAGAA AGCGAAAATG AGAGTAAGAT GAATGTGGAA 
GTGCTTGAGT GGGAGACGAA CAAAAAAACC CATCAAGACA TTTCTGAGGA ATTGAACAGA 
GAGCAAGATA CAAGTTTCCA TAAGCGCTTG CCTTATTTGG GCATTCGTGT ACTTCCTCCA 
GACACTCAGT CCCGCCCTCT TAGACGCATT TCTGGCCACT CATTGGAGGA GCCCGTAGGG 
AAGAATGAAT TCAATAGGAC GCCGTTTGCT TTCCTTACAA CTGAGCAGTC TGATTACGCA 
GAGTCGGAGT CAGGAGCCAG TGTGTCTGAG TGTAGCATAT CGGCAGCCAG TATCTCTGGT 
CTATCAGTGT ATATGACAGA AAGGTGTCAT TTAAATAGCA AACACAATTC CCAGGAGCCC 
TTACTTCCAG GCCTGTGGTT AAAACCGAGC TTGGAGAGAC TCATTAGAGA GGGTAAGGAG 
ATCAGGATGA TGGGGAAAGG TGGAGGAACA GAATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212997.1
CDS	408..4766
Translation	MSKVAVKKLHWRSKVQENFVPLVGSGELGIAIGGGADYGEFPFVTAAPGGGITVGDIILEIGGTPVLGLTLGDVRGLLNSCPHPIRIKTVSPGSTLCKDLRLYLSKCFTPGSMDSQLQQVIRENLYLRAVPCTTRQPRDGEISGVDYNFVSVEEFFSLEESGALLESGKFKEKKNGFESLEGNYYGTPRPVHIGPDSPPISYQEHRNLLRNFRTRSKSLSNLEKAGEEGDNSEEDSGLSGGSAGASSVPVHTSSPSQSRNLRGGNTSALENGLGPRRGARVRGSENWEMMYSDSGNAYYKDHHLKPSSSSWLNRRAHSRDNVCMPELAEFTDQPSELRGFSVHTRLVKGARGFGFNIVGGSRRREFLQIYSITPGGPSTLNTADILVYINDTCVLGYSHKDVVEMLKAIPIGHTVDIMVRRGYPMLYNSEGCPKMSNPRLTASQNQSPHLPPPTTTQPQAQAPLPYAQLHLNHNSPPWADFDRGSSRNLRVRASRFSLDANGNSPPFASPPRPPSSYQGSNGYGSGFLRPPRTARSIRRLQSAELASQSDSEVVSAIGSHRASFIRNHNNNSLSTPPPLRLHSFKSSESDLSSSTPPPISRLPLPHTELSPGPVSFSPGGGRRYSHLRKPQNSMLTPPSSVHSDGPYFTFNGTASASSGSPNSSIPSPGALSAVIGSGVGGMAAGGELVPVALAKCERGSNMGFSVTAGGHGGRQTLVKKVWDHRQCNGLQSGDAVVKINGADVQSLSFAQVQRILQEHTKKGEVILLVYRGPSNYSNSPSSVRRLTPPPPRSASDSEVPFTDSMPLSRNSLTPPSPALVRSSLVQSTSFLDSVPVTLTLEPRDWMSSEDGGTVLSPRVKTEEKEQPPALRGYEVELKRKPGEGFGFVIASQDVENVKAVSLLPHRFVTVRRGSPAARSGQIRPGDRLEAVDGRSVVNLPHRELAQILRRAGNTLRLTIVPRASAHSSERADYDPDYKNRKTHKSKPKQDATRYYSVELERGPTGFGFSLRGGSEYNMGLYVLGLMEGGPASRSHKIQVSDQLVEINGDSAAGMTHSQAVEQIRTGGSRIHLVFKRGNGYVPDYDRDHITAVSSSPQPLQSNLAAVSHHRPQRDATSSLQKPEKTNRGWKEPRERSDGGDRESPARLKGDRQGRRRRSRDDERRRRSYSLPRNTPRRHSDESMKVEKVERNGREMEKGNKRSVDRPKRSKSEERVRERKREGDESKNTQTINKHRLPQPKLQWEENEEEEEEWDGETEQEWTRERERLKDKKREREEIASLDSESDGELESENESKMNVEVLEWETNKKTHQDISEELNREQDTSFHKRLPYLGIRVLPPDTQSRPLRRISGHSLEEPVGKNEFNRTPFAFLTTEQSDYAESESGASVSECSISAASISGLSVYMTERCHLNSKHNSQEPLLPGLWLKPSLERLIREGKEIRMMGKGGGTEC

Target ORF information:

RefSeq Version	XM_017357508.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio novel protein similar to vertebrate membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1) (LOC556872), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357508.2

ORF Insert Sequence:

ATGTCTAAGG TTGCAGTAAA GAAGCTGCAC TGGCGGTCCA AAGTGCAGGA GAACTTCGTC 
CCACTGGTCG GCTCTGGAGA GTTGGGGATA GCCATCGGGG GCGGGGCCGA TTATGGAGAA 
TTCCCATTCG TCACGGCTGC ACCAGGGGGC GGGATCACAG TTGGCGATAT CATCTTAGAA 
ATTGGAGGGA CTCCTGTTTT GGGCCTGACT TTAGGAGATG TGCGTGGACT TCTCAACTCC 
TGCCCTCATC CGATCCGCAT CAAGACTGTT TCTCCAGGCT CAACTCTATG CAAGGATCTG 
AGATTATACT TGAGCAAATG TTTCACACCA GGATCTATGG ACAGTCAGCT CCAGCAGGTG 
ATCCGAGAAA ACCTCTACCT GAGAGCCGTG CCATGTACAA CTAGACAGCC ACGAGACGGT 
GAGATCTCTG GGGTCGACTA TAACTTTGTG TCAGTCGAAG AGTTCTTCTC TCTGGAGGAG 
TCGGGGGCGC TGTTGGAGAG CGGAAAGTTC AAAGAAAAAA AGAACGGCTT CGAATCACTT 
GAGGGAAATT ACTACGGGAC TCCTCGTCCG GTTCACATTG GCCCTGACAG CCCTCCCATC 
AGCTACCAGG AGCACCGCAA CCTGCTAAGA AACTTCCGCA CTCGCAGTAA ATCACTCAGC 
AACCTGGAGA AAGCAGGAGA GGAAGGCGAC AACAGTGAGG AGGACTCAGG TCTATCAGGT 
GGTTCAGCCG GGGCGAGCAG TGTCCCCGTC CACACGTCAT CCCCCAGCCA ATCACGAAAC 
TTAAGAGGTG GAAACACCAG TGCCTTGGAG AATGGGCTCG GACCCCGTAG AGGAGCACGG 
GTGAGAGGGT CGGAAAACTG GGAGATGATG TACAGTGACT CAGGGAATGC CTATTACAAA 
GACCATCATC TAAAGCCATC GTCCTCGTCC TGGCTCAATC GCAGAGCTCA CAGCAGAGAC 
AATGTCTGTA TGCCCGAATT AGCAGAGTTT ACGGACCAGC CGAGTGAACT GAGAGGTTTC 
TCCGTCCACA CTCGTCTAGT TAAAGGCGCA CGGGGCTTCG GTTTCAATAT AGTGGGAGGC 
AGCAGAAGGA GAGAGTTCCT GCAGATCTAC AGCATCACTC CCGGAGGACC TTCAACCCTG 
AACACAGCTG ACATCCTAGT CTACATCAAT GATACCTGTG TGCTCGGCTA CTCTCACAAA 
GATGTAGTAG AAATGCTAAA AGCCATCCCT ATTGGTCATA CTGTGGACAT CATGGTCCGA 
AGAGGTTACC CCATGCTCTA CAACTCAGAA GGTTGTCCTA AGATGTCCAA TCCAAGGCTG 
ACTGCATCCC AAAACCAGTC ACCACATCTA CCTCCACCAA CCACCACCCA ACCGCAGGCC 
CAGGCTCCTC TCCCATATGC ACAGCTCCAC CTCAACCACA ACAGCCCCCC GTGGGCTGAC 
TTTGACAGGG GGTCTTCGAG GAATCTCAGA GTGCGAGCTT CAAGGTTTAG TCTGGATGCT 
AATGGAAATT CACCTCCATT TGCTTCACCT CCAAGACCTC CGTCATCGTA CCAGGGCTCC 
AACGGGTATG GGAGTGGGTT TTTGAGACCG CCACGCACTG CGAGAAGCAT ACGGAGGTTG 
CAGAGCGCAG AACTGGCCAG CCAAAGTGAC AGTGAGGTGG TGTCTGCAAT CGGATCCCAC 
AGGGCCTCGT TTATCCGTAA CCACAACAAC AACTCTCTCT CCACTCCGCC ACCTCTCCGC 
CTGCACTCCT TCAAATCTTC AGAGAGTGAC CTTTCCTCAA GTACTCCACC GCCCATATCT 
CGATTACCTC TACCCCACAC TGAACTAAGC CCAGGTCCTG TCTCCTTTTC CCCTGGAGGT 
GGAAGACGCT ACTCGCATTT ACGTAAACCT CAGAATTCAA TGCTAACCCC ACCAAGCAGC 
GTCCATTCTG ACGGGCCTTA CTTCACCTTC AACGGGACGG CCAGCGCCAG CAGCGGCAGC 
CCAAACAGCA GCATTCCTTC ACCCGGAGCC CTGAGTGCCG TCATAGGCAG TGGTGTTGGA 
GGAATGGCGG CAGGTGGAGA ATTGGTTCCT GTTGCGTTGG CTAAATGCGA GAGGGGCAGC 
AATATGGGGT TCAGTGTGAC GGCGGGAGGG CACGGAGGCA GACAGACCCT TGTGAAGAAA 
GTCTGGGACC ACAGACAGTG TAATGGGCTG CAGTCTGGAG ATGCTGTTGT GAAGATCAAT 
GGAGCTGATG TACAGAGTCT CAGCTTTGCA CAGGTCCAAA GGATTTTGCA AGAACACACT 
AAAAAAGGAG AGGTTATTCT GCTGGTTTAC AGGGGCCCCT CAAACTATTC CAATTCGCCT 
AGTTCTGTGC GAAGACTAAC TCCTCCCCCT CCACGCTCTG CTTCCGACTC TGAAGTTCCT 
TTTACCGACT CCATGCCATT ATCTCGTAAC TCTCTGACCC CGCCATCTCC AGCGCTGGTG 
CGCTCTTCAT TGGTCCAGAG CACCAGCTTT CTGGATTCAG TACCTGTCAC TCTCACACTG 
GAGCCACGTG ATTGGATGAG CTCAGAGGAT GGAGGGACGG TGCTCAGTCC CAGAGTGAAA 
ACAGAGGAGA AAGAGCAGCC CCCGGCGCTG CGGGGCTATG AGGTGGAGCT GAAGAGGAAA 
CCAGGTGAAG GATTTGGATT CGTTATCGCC TCTCAGGATG TGGAAAATGT CAAAGCGGTG 
TCTCTGCTGC CGCACAGGTT TGTGACGGTG CGCAGAGGAA GCCCAGCGGC ACGCAGCGGT 
CAGATCAGGC CTGGCGATCG CCTGGAAGCT GTGGACGGCA GATCAGTGGT CAATTTACCT 
CACAGGGAGC TGGCTCAGAT CCTGAGGAGA GCTGGAAACA CTCTCCGCTT GACCATCGTA 
CCTCGCGCCA GTGCTCATTC CTCAGAGCGA GCAGATTATG ACCCTGATTA TAAGAACAGG 
AAAACTCACA AGTCAAAACC AAAGCAGGAT GCCACGCGGT ACTACAGTGT GGAACTGGAG 
CGGGGTCCCA CTGGATTCGG CTTTAGTCTG AGAGGAGGAA GTGAATACAA CATGGGCCTA 
TATGTGCTGG GACTGATGGA GGGAGGACCG GCCTCACGCA GCCACAAAAT ACAGGTGAGT 
GATCAGTTGG TGGAGATCAA CGGAGACAGT GCAGCAGGAA TGACACACAG TCAGGCAGTG 
GAGCAGATCA GGACTGGAGG AAGCAGGATT CACCTAGTCT TCAAAAGAGG AAACGGATAC 
GTTCCTGACT ACGACCGTGA TCACATCACA GCCGTCTCCT CCTCACCCCA GCCCCTTCAG 
TCGAATTTGG CTGCAGTGAG CCACCACCGG CCTCAACGGG ACGCCACATC CAGCCTTCAA 
AAACCAGAGA AGACCAACCG AGGGTGGAAA GAACCGAGGG AGAGGAGTGA CGGAGGAGAC 
CGCGAGAGCC CTGCAAGACT CAAGGGTGAC CGTCAGGGCA GAAGGCGAAG GTCAAGAGAT 
GATGAAAGGA GACGAAGGTC ATACAGCTTG CCTAGAAACA CACCAAGAAG ACACAGCGAT 
GAAAGCATGA AGGTTGAAAA GGTTGAAAGA AATGGGAGAG AGATGGAAAA AGGTAACAAG 
AGGAGCGTGG ACAGGCCAAA ACGTAGCAAG AGTGAGGAGA GGGTGAGAGA AAGAAAGAGA 
GAAGGAGATG AGAGTAAGAA CACACAAACC ATCAATAAAC ACAGACTTCC TCAGCCCAAA 
CTGCAGTGGG AGGAAAATGA GGAGGAGGAG GAGGAGTGGG ATGGAGAGAC TGAGCAAGAG 
TGGACAAGAG AGAGAGAAAG GCTAAAAGAC AAAAAACGGG AGAGGGAGGA GATAGCGTCT 
CTTGATTCAG AGAGCGACGG GGAATTAGAA AGCGAAAATG AGAGTAAGAT GAATGTGGAA 
GTGCTTGAGT GGGAGACGAA CAAAAAAACC CATCAAGACA TTTCTGAGGA ATTGAACAGA 
GAGCAAGATA CAAGTTTCCA TAAGCGCTTG CCTTATTTGG GCATTCGTGT ACTTCCTCCA 
GACACTCAGT CCCGCCCTCT TAGACGCATT TCTGGCCACT CATTGGAGGA GCCCGTAGGG 
AAGAATGAAT TCAATAGGAC GCCGTTTGCT TTCCTTACAA CTGAGCAGTC TGATTACGCA 
GAGTCGGAGT CAGGAGCCAG TGTGTCTGAG TGTAGCATAT CGGCAGCCAG TATCTCTGGT 
CTATCAGTGT ATATGACAGA AAGGTGTCAT TTAAATAGCA AACACAATTC CCAGGAGCCC 
TTACTTCCAG GCCTGTGGTT AAAACCGAGC TTGGAGAGAC TCATTAGAGA GGGTAAGGAG 
ATCAGGATGA TGGGGAAAGG TGGAGGAACA GAATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC556872 ( XM_017357508.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212997.1 Danio rerio novel protein similar to vertebrate membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1) (LOC556872), mRNA.

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CloneID	ODa60098
Clone ID Related Accession (Same CDS sequence)	XM_017357508.2 , XM_017357508.1
Accession Version	XM_017357508.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAAGG TTGCAGTAAA GAAGCTGCAC TGGCGGTCCA AAGTGCAGGA GAACTTCGTC 
CCACTGGTCG GCTCTGGAGA GTTGGGGATA GCCATCGGGG GCGGGGCCGA TTATGGAGAA 
TTCCCATTCG TCACGGCTGC ACCAGGGGGC GGGATCACAG TTGGCGATAT CATCTTAGAA 
ATTGGAGGGA CTCCTGTTTT GGGCCTGACT TTAGGAGATG TGCGTGGACT TCTCAACTCC 
TGCCCTCATC CGATCCGCAT CAAGACTGTT TCTCCAGGCT CAACTCTATG CAAGGATCTG 
AGATTATACT TGAGCAAATG TTTCACACCA GGATCTATGG ACAGTCAGCT CCAGCAGGTG 
ATCCGAGAAA ACCTCTACCT GAGAGCCGTG CCATGTACAA CTAGACAGCC ACGAGACGGT 
GAGATCTCTG GGGTCGACTA TAACTTTGTG TCAGTCGAAG AGTTCTTCTC TCTGGAGGAG 
TCGGGGGCGC TGTTGGAGAG CGGAAAGTTC AAAGAAAAAA AGAACGGCTT CGAATCACTT 
GAGGGAAATT ACTACGGGAC TCCTCGTCCG GTTCACATTG GCCCTGACAG CCCTCCCATC 
AGCTACCAGG AGCACCGCAA CCTGCTAAGA AACTTCCGCA CTCGCAGTAA ATCACTCAGC 
AACCTGGAGA AAGCAGGAGA GGAAGGCGAC AACAGTGAGG AGGACTCAGG TCTATCAGGT 
GGTTCAGCCG GGGCGAGCAG TGTCCCCGTC CACACGTCAT CCCCCAGCCA ATCACGAAAC 
TTAAGAGGTG GAAACACCAG TGCCTTGGAG AATGGGCTCG GACCCCGTAG AGGAGCACGG 
GTGAGAGGGT CGGAAAACTG GGAGATGATG TACAGTGACT CAGGGAATGC CTATTACAAA 
GACCATCATC TAAAGCCATC GTCCTCGTCC TGGCTCAATC GCAGAGCTCA CAGCAGAGAC 
AATGTCTGTA TGCCCGAATT AGCAGAGTTT ACGGACCAGC CGAGTGAACT GAGAGGTTTC 
TCCGTCCACA CTCGTCTAGT TAAAGGCGCA CGGGGCTTCG GTTTCAATAT AGTGGGAGGC 
AGCAGAAGGA GAGAGTTCCT GCAGATCTAC AGCATCACTC CCGGAGGACC TTCAACCCTG 
AACACAGCTG ACATCCTAGT CTACATCAAT GATACCTGTG TGCTCGGCTA CTCTCACAAA 
GATGTAGTAG AAATGCTAAA AGCCATCCCT ATTGGTCATA CTGTGGACAT CATGGTCCGA 
AGAGGTTACC CCATGCTCTA CAACTCAGAA GGTTGTCCTA AGATGTCCAA TCCAAGGCTG 
ACTGCATCCC AAAACCAGTC ACCACATCTA CCTCCACCAA CCACCACCCA ACCGCAGGCC 
CAGGCTCCTC TCCCATATGC ACAGCTCCAC CTCAACCACA ACAGCCCCCC GTGGGCTGAC 
TTTGACAGGG GGTCTTCGAG GAATCTCAGA GTGCGAGCTT CAAGGTTTAG TCTGGATGCT 
AATGGAAATT CACCTCCATT TGCTTCACCT CCAAGACCTC CGTCATCGTA CCAGGGCTCC 
AACGGGTATG GGAGTGGGTT TTTGAGACCG CCACGCACTG CGAGAAGCAT ACGGAGGTTG 
CAGAGCGCAG AACTGGCCAG CCAAAGTGAC AGTGAGGTGG TGTCTGCAAT CGGATCCCAC 
AGGGCCTCGT TTATCCGTAA CCACAACAAC AACTCTCTCT CCACTCCGCC ACCTCTCCGC 
CTGCACTCCT TCAAATCTTC AGAGAGTGAC CTTTCCTCAA GTACTCCACC GCCCATATCT 
CGATTACCTC TACCCCACAC TGAACTAAGC CCAGGTCCTG TCTCCTTTTC CCCTGGAGGT 
GGAAGACGCT ACTCGCATTT ACGTAAACCT CAGAATTCAA TGCTAACCCC ACCAAGCAGC 
GTCCATTCTG ACGGGCCTTA CTTCACCTTC AACGGGACGG CCAGCGCCAG CAGCGGCAGC 
CCAAACAGCA GCATTCCTTC ACCCGGAGCC CTGAGTGCCG TCATAGGCAG TGGTGTTGGA 
GGAATGGCGG CAGGTGGAGA ATTGGTTCCT GTTGCGTTGG CTAAATGCGA GAGGGGCAGC 
AATATGGGGT TCAGTGTGAC GGCGGGAGGG CACGGAGGCA GACAGACCCT TGTGAAGAAA 
GTCTGGGACC ACAGACAGTG TAATGGGCTG CAGTCTGGAG ATGCTGTTGT GAAGATCAAT 
GGAGCTGATG TACAGAGTCT CAGCTTTGCA CAGGTCCAAA GGATTTTGCA AGAACACACT 
AAAAAAGGAG AGGTTATTCT GCTGGTTTAC AGGGGCCCCT CAAACTATTC CAATTCGCCT 
AGTTCTGTGC GAAGACTAAC TCCTCCCCCT CCACGCTCTG CTTCCGACTC TGAAGTTCCT 
TTTACCGACT CCATGCCATT ATCTCGTAAC TCTCTGACCC CGCCATCTCC AGCGCTGGTG 
CGCTCTTCAT TGGTCCAGAG CACCAGCTTT CTGGATTCAG TACCTGTCAC TCTCACACTG 
GAGCCACGTG ATTGGATGAG CTCAGAGGAT GGAGGGACGG TGCTCAGTCC CAGAGTGAAA 
ACAGAGGAGA AAGAGCAGCC CCCGGCGCTG CGGGGCTATG AGGTGGAGCT GAAGAGGAAA 
CCAGGTGAAG GATTTGGATT CGTTATCGCC TCTCAGGATG TGGAAAATGT CAAAGCGGTG 
TCTCTGCTGC CGCACAGGTT TGTGACGGTG CGCAGAGGAA GCCCAGCGGC ACGCAGCGGT 
CAGATCAGGC CTGGCGATCG CCTGGAAGCT GTGGACGGCA GATCAGTGGT CAATTTACCT 
CACAGGGAGC TGGCTCAGAT CCTGAGGAGA GCTGGAAACA CTCTCCGCTT GACCATCGTA 
CCTCGCGCCA GTGCTCATTC CTCAGAGCGA GCAGATTATG ACCCTGATTA TAAGAACAGG 
AAAACTCACA AGTCAAAACC AAAGCAGGAT GCCACGCGGT ACTACAGTGT GGAACTGGAG 
CGGGGTCCCA CTGGATTCGG CTTTAGTCTG AGAGGAGGAA GTGAATACAA CATGGGCCTA 
TATGTGCTGG GACTGATGGA GGGAGGACCG GCCTCACGCA GCCACAAAAT ACAGGTGAGT 
GATCAGTTGG TGGAGATCAA CGGAGACAGT GCAGCAGGAA TGACACACAG TCAGGCAGTG 
GAGCAGATCA GGACTGGAGG AAGCAGGATT CACCTAGTCT TCAAAAGAGG AAACGGATAC 
GTTCCTGACT ACGACCGTGA TCACATCACA GCCGTCTCCT CCTCACCCCA GCCCCTTCAG 
TCGAATTTGG CTGCAGTGAG CCACCACCGG CCTCAACGGG ACGCCACATC CAGCCTTCAA 
AAACCAGAGA AGACCAACCG AGGGTGGAAA GAACCGAGGG AGAGGAGTGA CGGAGGAGAC 
CGCGAGAGCC CTGCAAGACT CAAGGGTGAC CGTCAGGGCA GAAGGCGAAG GTCAAGAGAT 
GATGAAAGGA GACGAAGGTC ATACAGCTTG CCTAGAAACA CACCAAGAAG ACACAGCGAT 
GAAAGCATGA AGGTTGAAAA GGTTGAAAGA AATGGGAGAG AGATGGAAAA AGGTAACAAG 
AGGAGCGTGG ACAGGCCAAA ACGTAGCAAG AGTGAGGAGA GGGTGAGAGA AAGAAAGAGA 
GAAGGAGATG AGAGTAAGAA CACACAAACC ATCAATAAAC ACAGACTTCC TCAGCCCAAA 
CTGCAGTGGG AGGAAAATGA GGAGGAGGAG GAGGAGTGGG ATGGAGAGAC TGAGCAAGAG 
TGGACAAGAG AGAGAGAAAG GCTAAAAGAC AAAAAACGGG AGAGGGAGGA GATAGCGTCT 
CTTGATTCAG AGAGCGACGG GGAATTAGAA AGCGAAAATG AGAGTAAGAT GAATGTGGAA 
GTGCTTGAGT GGGAGACGAA CAAAAAAACC CATCAAGACA TTTCTGAGGA ATTGAACAGA 
GAGCAAGATA CAAGTTTCCA TAAGCGCTTG CCTTATTTGG GCATTCGTGT ACTTCCTCCA 
GACACTCAGT CCCGCCCTCT TAGACGCATT TCTGGCCACT CATTGGAGGA GCCCGTAGGG 
AAGAATGAAT TCAATAGGAC GCCGTTTGCT TTCCTTACAA CTGAGCAGTC TGATTACGCA 
GAGTCGGAGT CAGGAGCCAG TGTGTCTGAG TGTAGCATAT CGGCAGCCAG TATCTCTGGT 
CTATCAGTGT ATATGACAGA AAGGTGTCAT TTAAATAGCA AACACAATTC CCAGGAGCCC 
TTACTTCCAG GCCTGTGGTT AAAACCGAGC TTGGAGAGAC TCATTAGAGA GGGTAAGGAG 
ATCAGGATGA TGGGGAAAGG TGGAGGAACA GAATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212997.1
CDS	300..4658
Translation	MSKVAVKKLHWRSKVQENFVPLVGSGELGIAIGGGADYGEFPFVTAAPGGGITVGDIILEIGGTPVLGLTLGDVRGLLNSCPHPIRIKTVSPGSTLCKDLRLYLSKCFTPGSMDSQLQQVIRENLYLRAVPCTTRQPRDGEISGVDYNFVSVEEFFSLEESGALLESGKFKEKKNGFESLEGNYYGTPRPVHIGPDSPPISYQEHRNLLRNFRTRSKSLSNLEKAGEEGDNSEEDSGLSGGSAGASSVPVHTSSPSQSRNLRGGNTSALENGLGPRRGARVRGSENWEMMYSDSGNAYYKDHHLKPSSSSWLNRRAHSRDNVCMPELAEFTDQPSELRGFSVHTRLVKGARGFGFNIVGGSRRREFLQIYSITPGGPSTLNTADILVYINDTCVLGYSHKDVVEMLKAIPIGHTVDIMVRRGYPMLYNSEGCPKMSNPRLTASQNQSPHLPPPTTTQPQAQAPLPYAQLHLNHNSPPWADFDRGSSRNLRVRASRFSLDANGNSPPFASPPRPPSSYQGSNGYGSGFLRPPRTARSIRRLQSAELASQSDSEVVSAIGSHRASFIRNHNNNSLSTPPPLRLHSFKSSESDLSSSTPPPISRLPLPHTELSPGPVSFSPGGGRRYSHLRKPQNSMLTPPSSVHSDGPYFTFNGTASASSGSPNSSIPSPGALSAVIGSGVGGMAAGGELVPVALAKCERGSNMGFSVTAGGHGGRQTLVKKVWDHRQCNGLQSGDAVVKINGADVQSLSFAQVQRILQEHTKKGEVILLVYRGPSNYSNSPSSVRRLTPPPPRSASDSEVPFTDSMPLSRNSLTPPSPALVRSSLVQSTSFLDSVPVTLTLEPRDWMSSEDGGTVLSPRVKTEEKEQPPALRGYEVELKRKPGEGFGFVIASQDVENVKAVSLLPHRFVTVRRGSPAARSGQIRPGDRLEAVDGRSVVNLPHRELAQILRRAGNTLRLTIVPRASAHSSERADYDPDYKNRKTHKSKPKQDATRYYSVELERGPTGFGFSLRGGSEYNMGLYVLGLMEGGPASRSHKIQVSDQLVEINGDSAAGMTHSQAVEQIRTGGSRIHLVFKRGNGYVPDYDRDHITAVSSSPQPLQSNLAAVSHHRPQRDATSSLQKPEKTNRGWKEPRERSDGGDRESPARLKGDRQGRRRRSRDDERRRRSYSLPRNTPRRHSDESMKVEKVERNGREMEKGNKRSVDRPKRSKSEERVRERKREGDESKNTQTINKHRLPQPKLQWEENEEEEEEWDGETEQEWTRERERLKDKKREREEIASLDSESDGELESENESKMNVEVLEWETNKKTHQDISEELNREQDTSFHKRLPYLGIRVLPPDTQSRPLRRISGHSLEEPVGKNEFNRTPFAFLTTEQSDYAESESGASVSECSISAASISGLSVYMTERCHLNSKHNSQEPLLPGLWLKPSLERLIREGKEIRMMGKGGGTEC

Target ORF information:

RefSeq Version	XM_017357508.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio novel protein similar to vertebrate membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1) (LOC556872), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357508.1

ORF Insert Sequence:

ATGTCTAAGG TTGCAGTAAA GAAGCTGCAC TGGCGGTCCA AAGTGCAGGA GAACTTCGTC 
CCACTGGTCG GCTCTGGAGA GTTGGGGATA GCCATCGGGG GCGGGGCCGA TTATGGAGAA 
TTCCCATTCG TCACGGCTGC ACCAGGGGGC GGGATCACAG TTGGCGATAT CATCTTAGAA 
ATTGGAGGGA CTCCTGTTTT GGGCCTGACT TTAGGAGATG TGCGTGGACT TCTCAACTCC 
TGCCCTCATC CGATCCGCAT CAAGACTGTT TCTCCAGGCT CAACTCTATG CAAGGATCTG 
AGATTATACT TGAGCAAATG TTTCACACCA GGATCTATGG ACAGTCAGCT CCAGCAGGTG 
ATCCGAGAAA ACCTCTACCT GAGAGCCGTG CCATGTACAA CTAGACAGCC ACGAGACGGT 
GAGATCTCTG GGGTCGACTA TAACTTTGTG TCAGTCGAAG AGTTCTTCTC TCTGGAGGAG 
TCGGGGGCGC TGTTGGAGAG CGGAAAGTTC AAAGAAAAAA AGAACGGCTT CGAATCACTT 
GAGGGAAATT ACTACGGGAC TCCTCGTCCG GTTCACATTG GCCCTGACAG CCCTCCCATC 
AGCTACCAGG AGCACCGCAA CCTGCTAAGA AACTTCCGCA CTCGCAGTAA ATCACTCAGC 
AACCTGGAGA AAGCAGGAGA GGAAGGCGAC AACAGTGAGG AGGACTCAGG TCTATCAGGT 
GGTTCAGCCG GGGCGAGCAG TGTCCCCGTC CACACGTCAT CCCCCAGCCA ATCACGAAAC 
TTAAGAGGTG GAAACACCAG TGCCTTGGAG AATGGGCTCG GACCCCGTAG AGGAGCACGG 
GTGAGAGGGT CGGAAAACTG GGAGATGATG TACAGTGACT CAGGGAATGC CTATTACAAA 
GACCATCATC TAAAGCCATC GTCCTCGTCC TGGCTCAATC GCAGAGCTCA CAGCAGAGAC 
AATGTCTGTA TGCCCGAATT AGCAGAGTTT ACGGACCAGC CGAGTGAACT GAGAGGTTTC 
TCCGTCCACA CTCGTCTAGT TAAAGGCGCA CGGGGCTTCG GTTTCAATAT AGTGGGAGGC 
AGCAGAAGGA GAGAGTTCCT GCAGATCTAC AGCATCACTC CCGGAGGACC TTCAACCCTG 
AACACAGCTG ACATCCTAGT CTACATCAAT GATACCTGTG TGCTCGGCTA CTCTCACAAA 
GATGTAGTAG AAATGCTAAA AGCCATCCCT ATTGGTCATA CTGTGGACAT CATGGTCCGA 
AGAGGTTACC CCATGCTCTA CAACTCAGAA GGTTGTCCTA AGATGTCCAA TCCAAGGCTG 
ACTGCATCCC AAAACCAGTC ACCACATCTA CCTCCACCAA CCACCACCCA ACCGCAGGCC 
CAGGCTCCTC TCCCATATGC ACAGCTCCAC CTCAACCACA ACAGCCCCCC GTGGGCTGAC 
TTTGACAGGG GGTCTTCGAG GAATCTCAGA GTGCGAGCTT CAAGGTTTAG TCTGGATGCT 
AATGGAAATT CACCTCCATT TGCTTCACCT CCAAGACCTC CGTCATCGTA CCAGGGCTCC 
AACGGGTATG GGAGTGGGTT TTTGAGACCG CCACGCACTG CGAGAAGCAT ACGGAGGTTG 
CAGAGCGCAG AACTGGCCAG CCAAAGTGAC AGTGAGGTGG TGTCTGCAAT CGGATCCCAC 
AGGGCCTCGT TTATCCGTAA CCACAACAAC AACTCTCTCT CCACTCCGCC ACCTCTCCGC 
CTGCACTCCT TCAAATCTTC AGAGAGTGAC CTTTCCTCAA GTACTCCACC GCCCATATCT 
CGATTACCTC TACCCCACAC TGAACTAAGC CCAGGTCCTG TCTCCTTTTC CCCTGGAGGT 
GGAAGACGCT ACTCGCATTT ACGTAAACCT CAGAATTCAA TGCTAACCCC ACCAAGCAGC 
GTCCATTCTG ACGGGCCTTA CTTCACCTTC AACGGGACGG CCAGCGCCAG CAGCGGCAGC 
CCAAACAGCA GCATTCCTTC ACCCGGAGCC CTGAGTGCCG TCATAGGCAG TGGTGTTGGA 
GGAATGGCGG CAGGTGGAGA ATTGGTTCCT GTTGCGTTGG CTAAATGCGA GAGGGGCAGC 
AATATGGGGT TCAGTGTGAC GGCGGGAGGG CACGGAGGCA GACAGACCCT TGTGAAGAAA 
GTCTGGGACC ACAGACAGTG TAATGGGCTG CAGTCTGGAG ATGCTGTTGT GAAGATCAAT 
GGAGCTGATG TACAGAGTCT CAGCTTTGCA CAGGTCCAAA GGATTTTGCA AGAACACACT 
AAAAAAGGAG AGGTTATTCT GCTGGTTTAC AGGGGCCCCT CAAACTATTC CAATTCGCCT 
AGTTCTGTGC GAAGACTAAC TCCTCCCCCT CCACGCTCTG CTTCCGACTC TGAAGTTCCT 
TTTACCGACT CCATGCCATT ATCTCGTAAC TCTCTGACCC CGCCATCTCC AGCGCTGGTG 
CGCTCTTCAT TGGTCCAGAG CACCAGCTTT CTGGATTCAG TACCTGTCAC TCTCACACTG 
GAGCCACGTG ATTGGATGAG CTCAGAGGAT GGAGGGACGG TGCTCAGTCC CAGAGTGAAA 
ACAGAGGAGA AAGAGCAGCC CCCGGCGCTG CGGGGCTATG AGGTGGAGCT GAAGAGGAAA 
CCAGGTGAAG GATTTGGATT CGTTATCGCC TCTCAGGATG TGGAAAATGT CAAAGCGGTG 
TCTCTGCTGC CGCACAGGTT TGTGACGGTG CGCAGAGGAA GCCCAGCGGC ACGCAGCGGT 
CAGATCAGGC CTGGCGATCG CCTGGAAGCT GTGGACGGCA GATCAGTGGT CAATTTACCT 
CACAGGGAGC TGGCTCAGAT CCTGAGGAGA GCTGGAAACA CTCTCCGCTT GACCATCGTA 
CCTCGCGCCA GTGCTCATTC CTCAGAGCGA GCAGATTATG ACCCTGATTA TAAGAACAGG 
AAAACTCACA AGTCAAAACC AAAGCAGGAT GCCACGCGGT ACTACAGTGT GGAACTGGAG 
CGGGGTCCCA CTGGATTCGG CTTTAGTCTG AGAGGAGGAA GTGAATACAA CATGGGCCTA 
TATGTGCTGG GACTGATGGA GGGAGGACCG GCCTCACGCA GCCACAAAAT ACAGGTGAGT 
GATCAGTTGG TGGAGATCAA CGGAGACAGT GCAGCAGGAA TGACACACAG TCAGGCAGTG 
GAGCAGATCA GGACTGGAGG AAGCAGGATT CACCTAGTCT TCAAAAGAGG AAACGGATAC 
GTTCCTGACT ACGACCGTGA TCACATCACA GCCGTCTCCT CCTCACCCCA GCCCCTTCAG 
TCGAATTTGG CTGCAGTGAG CCACCACCGG CCTCAACGGG ACGCCACATC CAGCCTTCAA 
AAACCAGAGA AGACCAACCG AGGGTGGAAA GAACCGAGGG AGAGGAGTGA CGGAGGAGAC 
CGCGAGAGCC CTGCAAGACT CAAGGGTGAC CGTCAGGGCA GAAGGCGAAG GTCAAGAGAT 
GATGAAAGGA GACGAAGGTC ATACAGCTTG CCTAGAAACA CACCAAGAAG ACACAGCGAT 
GAAAGCATGA AGGTTGAAAA GGTTGAAAGA AATGGGAGAG AGATGGAAAA AGGTAACAAG 
AGGAGCGTGG ACAGGCCAAA ACGTAGCAAG AGTGAGGAGA GGGTGAGAGA AAGAAAGAGA 
GAAGGAGATG AGAGTAAGAA CACACAAACC ATCAATAAAC ACAGACTTCC TCAGCCCAAA 
CTGCAGTGGG AGGAAAATGA GGAGGAGGAG GAGGAGTGGG ATGGAGAGAC TGAGCAAGAG 
TGGACAAGAG AGAGAGAAAG GCTAAAAGAC AAAAAACGGG AGAGGGAGGA GATAGCGTCT 
CTTGATTCAG AGAGCGACGG GGAATTAGAA AGCGAAAATG AGAGTAAGAT GAATGTGGAA 
GTGCTTGAGT GGGAGACGAA CAAAAAAACC CATCAAGACA TTTCTGAGGA ATTGAACAGA 
GAGCAAGATA CAAGTTTCCA TAAGCGCTTG CCTTATTTGG GCATTCGTGT ACTTCCTCCA 
GACACTCAGT CCCGCCCTCT TAGACGCATT TCTGGCCACT CATTGGAGGA GCCCGTAGGG 
AAGAATGAAT TCAATAGGAC GCCGTTTGCT TTCCTTACAA CTGAGCAGTC TGATTACGCA 
GAGTCGGAGT CAGGAGCCAG TGTGTCTGAG TGTAGCATAT CGGCAGCCAG TATCTCTGGT 
CTATCAGTGT ATATGACAGA AAGGTGTCAT TTAAATAGCA AACACAATTC CCAGGAGCCC 
TTACTTCCAG GCCTGTGGTT AAAACCGAGC TTGGAGAGAC TCATTAGAGA GGGTAAGGAG 
ATCAGGATGA TGGGGAAAGG TGGAGGAACA GAATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC556872 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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