akna gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_009295258.3	XP_009293533.1	AT-hook-containing transcription factor-like isoform X1
XM_009295259.3	XP_009293534.1	AT-hook-containing transcription factor-like isoform X2
XM_009295258.2	XP_009293533.1	AT-hook-containing transcription factor-like isoform X1
XM_009295256.3	XP_009293531.1	AT-hook-containing transcription factor-like isoform X1
XM_005161116.4	XP_005161173.1	AT-hook-containing transcription factor-like isoform X1
XM_678843.6	XP_683935.4	AT-hook-containing transcription factor-like isoform X1
XM_005161116.3	XP_005161173.1	AT-hook-containing transcription factor-like isoform X1
XM_009295257.2	XP_009293532.1	AT-hook-containing transcription factor-like isoform X1
XM_009295259.2	XP_009293534.1	AT-hook-containing transcription factor-like isoform X3
XM_678843.7	XP_683935.4	AT-hook-containing transcription factor-like isoform X1
XM_017353013.1	XP_017208502.1	AT-hook-containing transcription factor-like isoform X2
XM_009295256.2	XP_009293531.1	AT-hook-containing transcription factor-like isoform X1
XM_009295257.3	XP_009293532.1	AT-hook-containing transcription factor-like isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa25697	XM_009295256.3 Latest version!	Danio rerio AT-hook transcription factor (akna), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa25697	XM_009295257.3 Latest version!	Danio rerio AT-hook transcription factor (akna), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa25697	XM_017353013.1 Latest version!	Danio rerio AT-hook transcription factor (akna), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa25697	XM_009295258.3 Latest version!	Danio rerio AT-hook transcription factor (akna), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa25697	XM_678843.7 Latest version!	Danio rerio AT-hook transcription factor (akna), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa25697	XM_005161116.4 Latest version!	Danio rerio AT-hook transcription factor (akna), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa42303	XM_009295259.3 Latest version!	Danio rerio AT-hook transcription factor (akna), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
ODa25697	XM_009295258.2	Danio rerio AT-hook transcription factor (akna), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa25697	XM_005161116.3	Danio rerio AT-hook transcription factor (akna), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa42303	XM_009295259.2	Danio rerio AT-hook transcription factor (akna), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa25697	XM_678843.6	Danio rerio AT-hook transcription factor (akna), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa25697	XM_009295256.2	Danio rerio AT-hook transcription factor (akna), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa25697	XM_009295257.2	Danio rerio AT-hook transcription factor (akna), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
Hide Old Accession Versions >>

akna ( XM_009295258.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293533.1 Danio rerio AT-hook transcription factor (akna), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa25697
Clone ID Related Accession (Same CDS sequence)	XM_009295258.3 , XM_009295258.2 , XM_009295256.3 , XM_005161116.4 , XM_678843.6 , XM_005161116.3 , XM_009295257.2 , XM_678843.7 , XM_017353013.1 , XM_009295256.2 , XM_009295257.3
Accession Version	XM_009295258.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	AT-hook-containing transcription factor-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009295258.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGGA GCAGAGGAGG CACCTCTACA GGGGTTCTGG TTTGGACACC TGCCCCACAG 
CTTTCCTCTC CCTCAGCCTC AGAGGGGTCA GACTTCAGCT GGGATGAAGA ACCGGACCAG 
GACTTCCAAA GCCAGATGGA TGAGAATGGG ATCATTGGTC TGGCAGAGAG GCAAGGGCAG 
CAGGAGAGAG AAGAGGAGGA GGTTGCAGAG GAGCTGTTGT GGGATGTGAA GACCCCTGAG 
CCTGAGCATG GGCCGCCGCC TGCAGTAGAG GAGATAAGCT GTCATCTGAG CGAACTGCTG 
GACTCTGAAC CGCTCTCTCA GGCCACTGGA GACCACTACT ACTCTCCATC ACTGCAGGAA 
GAACACCAAA CAGACGATGA TCGTAGTGTG TTGTCTGAGG ATTGGGCTGA TGACGAGGGT 
GGTACAGGGA GCCCAGTAAA GCACAAATTA AGCTCTTTCC CTGAGAGAGA CACTGCATTA 
GACATGACCG ATGAGGAGAC AGAAGAGAAA AACACACAAG AAGATGCTCA GAGATTCATG 
GATCAATCAT TTCATGTCAC AGAGGAGAGG GCAGCTGTGT TTCAAACTCT GAAAACCCTT 
GATTTCAGTC AGGAAATGAG TGGGATTTTC AAAGATCATC CAATTTCAGG AGTCCCAGAG 
TACTCTGATC AAAGTGACTG CGAGGAACTT GATGCCCGAT TTCTTCCAGT CTCGCATCAT 
TTCTCTAGAA ACACTTCCAA TCCCACCAGT TTTCCCCATT TATCCTCTGA GGAACTGATG 
AATGACTGTG GGATTGAAGC TGAGACGTTA CCTGAGCTAG AAATTAGGTC AGATTCATCT 
CTTGGCACTT ACCAGAGTCC TGTCTCTAAA ACAAACCACA CTTTCAGATC AAACCAGGAA 
GAGATTAAAC ACAAGATCTC ACCTCAAACT TCACCCAGAA GCCCTGTAAT AAAAAACAGC 
CCTGAAAAAA TATACCAAGA TGCTACGAGT GGCGAAAAAT TAGATGGAAG GTTACACAGG 
CCTTACCACA ATCTGCAGCC TCCAACAGTC TCTCCCAGAA AAACAACCTC TGCTTCCTGC 
AACACTCAGG TTTCCATTCC AGCGCAGGCT ATTTCTAAAG CAAGCGTCTC CCCCTCAGAG 
CGATCGCCTC CTCCTACACC TCACAGAAAT TCAGCAATAA AGAATCACAG AAACTGCAAA 
ACCTCTCACA TGGATCCAGA TGACATCAGG AAAGGACAGC TGAATCATGC CTTGCCTGAT 
TTCTCCAAAG TAGAGCCGAA GGTGCGTTTC CCAAAAAGAA GTGAATACAA ACCTCCCAAA 
AGCCGAAGAC CCCCTCATGT TCGAACATCA TACCCGGGTC TTCCAGTGGT GTTCAAATCT 
CCTGCTGAGA TTGTGAAAGA GGTCCTGTTG AGCAGCTGTG ATGGGACTAC TGGGAGTCCA 
TCCTCCAGCG AGACTCAGAA GCACCTGCAC AATATCGTCC CAGAAGAGTT TCGCTGCCCA 
CAGCAGGCCA GCACACTGAT GCAGCAGCTA CAGGAGGACT ATAACAGGCT TCTTACCAAG 
TATGCAGAAG CTGAAAATAC TATTGATCGA CTTCGCCTTG AAGCAAAGGT GGGTCTCTCT 
TCGGAACCAC CCAAACCCAG TAGTACAGCT GTCCTGTCGG GAGTTATTCA GGAAGGGTCA 
AAGGTCATAA CCATCAGTTT TCCTCAAGCA CAGAGGGCAG AGTTCAGTAC AGGTTCTGTT 
CATCTGACCC AACAGAAGGA GCTCTCAGAA AACTCCAGAA AAGCAGCAAC TCGGCCTTCT 
TCTGTAAGCT CTATCAGCTC GAGGAGGTCT GGATCTCTTA CTCCAGACCA TCTGACAGAC 
ACTCTGTCCA AACAGAACCA AAGATTTCAG CTACAGGTGG ATGCATTTGA GTCGCTTTTA 
AAGGGTGGGA AGCTCAAGCC ATGTGAGCAG ACAAAGGGTC TCTCCACACT GGCTGAGGGG 
CAGGAGTCTC TTGAGAGGGC TTATCTAAAT GCACGAGTCC AATATCAGAT GCTGCAGCAA 
CGACAAGGCA GACATGCCAT CTTTGACCCA GACAGGGATC TAGAGGGTCA GATCTTCAGG 
TCAGGGATGC GATTAGAGGA GCTGAAAGAG TGGCTGGAAC AAGCAGAACA GAACCAACCT 
GTTTCTGAAC CTACTTTATC CCCATATCCT CCTTCAGATG TCCTTTCTGC ATCCATGCTG 
GAGCCTGAAC CAGTGCCTGA GATCCCCCTG TCAGCAGTCT GTCCTGAGGC TTGTGTTGGT 
GTGGAGGTCA GTTCAGTCAG TGGAGAGAGT GATAGTGGTA GAGAGGAAAT GGAAATGCTT 
CCGTTTGTTC TTCATCCCCT CGATAACAAA CACCAACGTG TGGAGAAAGA TTTCAGTGAA 
CTCATGGACT GCTATCAGAG TGTTAAAGAG CTCCCCGGGA CACTGGATCA GGCTGTGACT 
TTAAAGAGCC ATGACTTGCT GGATTTTGGA AGCTCTGTTC CTCCTGGGAA AAAAAGTGTA 
ACGGACGGAC AACCACTCAA GACTGTTAAT GAGAGAGAGA TGAGAAAACC AGCTCAGCAG 
CAGCCGGCAG CAGGTTGTGT CCCTCCCTCA TGCCCATCTG TCAGTTCACA GGTGTGCGAC 
TCTATGGAAA CTCACCCAGA GGCTTTTTGC CACTCCTATG TATTGGCAGG AGAATCGACC 
AGAGAGAGTA AAACAGCAGG AAACATGAAA GCTCAAAGCA CTAGCTTGAC CAGCCTGGAA 
GAGAGAACAC ACCACAGAAC TGTAGGCCTG AAAGCGAAGG CGAAGACTCT AAGAGCGCCC 
CCTATGGATG GGGTCATGTC TCCAGAAACA GACAGTGGCT TTATGGGCTC TGAAAGCAGT 
CAGCTCACTG CTGCATTACA CAGTCCTCTC CACCAGAGGG CAGTAATAAG TTCCATCAGA 
CTGTCTGACC CTCACACAAA GGACACAGAG AGACCAGAGT CTGCTTCTCC TTCTGGGCAG 
CCTTTCATTG TCTCTCCTTC TCCATCCCAA CCTTCTCCAA ACACCACAAA AAAGCACATT 
GATACATCAG GAAAATGCAC AGGTCCTTAT AAGAAAATAG GAGGGGAGGA AAGGTGTTCT 
TTTTCCAGTC TTTCTTCCTC CTTATCCCCC CTTCACTGGC CACGTAGCAG CATTCTGCCC 
TGGACAAGCA GCCTGACCAG TCAATCAGAG CGTGGAGCTG ATGAGGAAGA AGAAAAAGCA 
CAGAAAGACA AATACCCACA ACCGACCAAT CAGCAACTCC GCTCTCACAG AAGCCCCTCC 
CTCCACAGGC CTTATCACCA CAGTTCTGCT CAGCTAACAA ATCACCAAAA GGCTCTTCAA 
TCTCTGCAAA AAGAAGTGAA CCGATTGAAA GAGAGATTAG AGGGAAATCT GAGACTATCA 
AAACCTGCCA GACCTATGAG AACAGAAGTT TCTGCCTCTG AAGACACCAG AGGTCACACA 
CATCCACAGA CATCAACACC TCAGAGGCAT GATTTAAGGT CTTCAAAGAG GAGGAGAAGG 
CAGACAGAGA GAGCTGGCAA TGGAGAAAAA GAGAGAGGAA TTTCACCTAG ACCAGTCCTC 
AGACAAAGAT CAGCATCTTT ACCTCGACAT CGGCCTGAAA CTGATATGAC ATCTGATTCT 
GAGCATGCTC AATCTGAGCC AAGACCATCA ACGTCTAGAT ATGTCCCAGT GTCGCCTGTG 
AAATCAAGAG GAAGAAGACA AACCAGGAGA TTTGAATGTC ACAAAGAGGA TAGTACATCA 
GGCAGATCTG ACAACAGCGA TGAAGCAGAA CCAAACGGAG GAGCAGAATC TCTGTGTCCT 
GATTGCATAT CCGATCGTAC CAGTGCTTTG ACACGTCTCC TGAGGAGCAG GAGCTCTCCA 
CATCTTGCAC AGACTTCCTG TAGCCATTGC CCATTGTGTG GTGCATTACA AGCCATTCAA 
AGAGCCTCTA AGCCAGCTCC AGTCTCCAGT CCCAGAGCGA GTCAGCCAAT GAGATCCTTA 
CAGAGAGAAA AAGGAGGCGT GAACGTGACA GTGGCTCCTC CCCCTTTATT GCAGAGAGCT 
GTTCCAGTTG TCCCATATGT GCCTGTATAT CCTTCAACTC TATATTTCTC CAGCCCAATG 
ACTCCACCGG CATACCCACA ACCCCTCAAC ATCTCATCAA ATCCCGTCAG GCCTGAGAGG 
TCAGGGGTCA GAGGTCACCG CAGACGCTCT TCCTCCATGG AGTATCATCA GGATTCTATG 
AGCAGCGCTT TGACCCGTGC CATCACTGCA GCGAGACACA TGAGAGAAGC CTCACAGCGC 
ATGGCCAGCA GCCTCCACAC TGCATCCTGC AGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293533.1
CDS	219..4577
Translation	MERSRGGTSTGVLVWTPAPQLSSPSASEGSDFSWDEEPDQDFQSQMDENGIIGLAERQGQQEREEEEVAEELLWDVKTPEPEHGPPPAVEEISCHLSELLDSEPLSQATGDHYYSPSLQEEHQTDDDRSVLSEDWADDEGGTGSPVKHKLSSFPERDTALDMTDEETEEKNTQEDAQRFMDQSFHVTEERAAVFQTLKTLDFSQEMSGIFKDHPISGVPEYSDQSDCEELDARFLPVSHHFSRNTSNPTSFPHLSSEELMNDCGIEAETLPELEIRSDSSLGTYQSPVSKTNHTFRSNQEEIKHKISPQTSPRSPVIKNSPEKIYQDATSGEKLDGRLHRPYHNLQPPTVSPRKTTSASCNTQVSIPAQAISKASVSPSERSPPPTPHRNSAIKNHRNCKTSHMDPDDIRKGQLNHALPDFSKVEPKVRFPKRSEYKPPKSRRPPHVRTSYPGLPVVFKSPAEIVKEVLLSSCDGTTGSPSSSETQKHLHNIVPEEFRCPQQASTLMQQLQEDYNRLLTKYAEAENTIDRLRLEAKVGLSSEPPKPSSTAVLSGVIQEGSKVITISFPQAQRAEFSTGSVHLTQQKELSENSRKAATRPSSVSSISSRRSGSLTPDHLTDTLSKQNQRFQLQVDAFESLLKGGKLKPCEQTKGLSTLAEGQESLERAYLNARVQYQMLQQRQGRHAIFDPDRDLEGQIFRSGMRLEELKEWLEQAEQNQPVSEPTLSPYPPSDVLSASMLEPEPVPEIPLSAVCPEACVGVEVSSVSGESDSGREEMEMLPFVLHPLDNKHQRVEKDFSELMDCYQSVKELPGTLDQAVTLKSHDLLDFGSSVPPGKKSVTDGQPLKTVNEREMRKPAQQQPAAGCVPPSCPSVSSQVCDSMETHPEAFCHSYVLAGESTRESKTAGNMKAQSTSLTSLEERTHHRTVGLKAKAKTLRAPPMDGVMSPETDSGFMGSESSQLTAALHSPLHQRAVISSIRLSDPHTKDTERPESASPSGQPFIVSPSPSQPSPNTTKKHIDTSGKCTGPYKKIGGEERCSFSSLSSSLSPLHWPRSSILPWTSSLTSQSERGADEEEEKAQKDKYPQPTNQQLRSHRSPSLHRPYHHSSAQLTNHQKALQSLQKEVNRLKERLEGNLRLSKPARPMRTEVSASEDTRGHTHPQTSTPQRHDLRSSKRRRRQTERAGNGEKERGISPRPVLRQRSASLPRHRPETDMTSDSEHAQSEPRPSTSRYVPVSPVKSRGRRQTRRFECHKEDSTSGRSDNSDEAEPNGGAESLCPDCISDRTSALTRLLRSRSSPHLAQTSCSHCPLCGALQAIQRASKPAPVSSPRASQPMRSLQREKGGVNVTVAPPPLLQRAVPVVPYVPVYPSTLYFSSPMTPPAYPQPLNISSNPVRPERSGVRGHRRRSSSMEYHQDSMSSALTRAITAARHMREASQRMASSLHTASCSY

Target ORF information:

RefSeq Version	XM_009295258.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio AT-hook transcription factor (akna), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295258.3

ORF Insert Sequence:

ATGGAGCGGA GCAGAGGAGG CACCTCTACA GGGGTTCTGG TTTGGACACC TGCCCCACAG 
CTTTCCTCTC CCTCAGCCTC AGAGGGGTCA GACTTCAGCT GGGATGAAGA ACCGGACCAG 
GACTTCCAAA GCCAGATGGA TGAGAATGGG ATCATTGGTC TGGCAGAGAG GCAAGGGCAG 
CAGGAGAGAG AAGAGGAGGA GGTTGCAGAG GAGCTGTTGT GGGATGTGAA GACCCCTGAG 
CCTGAGCATG GGCCGCCGCC TGCAGTAGAG GAGATAAGCT GTCATCTGAG CGAACTGCTG 
GACTCTGAAC CGCTCTCTCA GGCCACTGGA GACCACTACT ACTCTCCATC ACTGCAGGAA 
GAACACCAAA CAGACGATGA TCGTAGTGTG TTGTCTGAGG ATTGGGCTGA TGACGAGGGT 
GGTACAGGGA GCCCAGTAAA GCACAAATTA AGCTCTTTCC CTGAGAGAGA CACTGCATTA 
GACATGACCG ATGAGGAGAC AGAAGAGAAA AACACACAAG AAGATGCTCA GAGATTCATG 
GATCAATCAT TTCATGTCAC AGAGGAGAGG GCAGCTGTGT TTCAAACTCT GAAAACCCTT 
GATTTCAGTC AGGAAATGAG TGGGATTTTC AAAGATCATC CAATTTCAGG AGTCCCAGAG 
TACTCTGATC AAAGTGACTG CGAGGAACTT GATGCCCGAT TTCTTCCAGT CTCGCATCAT 
TTCTCTAGAA ACACTTCCAA TCCCACCAGT TTTCCCCATT TATCCTCTGA GGAACTGATG 
AATGACTGTG GGATTGAAGC TGAGACGTTA CCTGAGCTAG AAATTAGGTC AGATTCATCT 
CTTGGCACTT ACCAGAGTCC TGTCTCTAAA ACAAACCACA CTTTCAGATC AAACCAGGAA 
GAGATTAAAC ACAAGATCTC ACCTCAAACT TCACCCAGAA GCCCTGTAAT AAAAAACAGC 
CCTGAAAAAA TATACCAAGA TGCTACGAGT GGCGAAAAAT TAGATGGAAG GTTACACAGG 
CCTTACCACA ATCTGCAGCC TCCAACAGTC TCTCCCAGAA AAACAACCTC TGCTTCCTGC 
AACACTCAGG TTTCCATTCC AGCGCAGGCT ATTTCTAAAG CAAGCGTCTC CCCCTCAGAG 
CGATCGCCTC CTCCTACACC TCACAGAAAT TCAGCAATAA AGAATCACAG AAACTGCAAA 
ACCTCTCACA TGGATCCAGA TGACATCAGG AAAGGACAGC TGAATCATGC CTTGCCTGAT 
TTCTCCAAAG TAGAGCCGAA GGTGCGTTTC CCAAAAAGAA GTGAATACAA ACCTCCCAAA 
AGCCGAAGAC CCCCTCATGT TCGAACATCA TACCCGGGTC TTCCAGTGGT GTTCAAATCT 
CCTGCTGAGA TTGTGAAAGA GGTCCTGTTG AGCAGCTGTG ATGGGACTAC TGGGAGTCCA 
TCCTCCAGCG AGACTCAGAA GCACCTGCAC AATATCGTCC CAGAAGAGTT TCGCTGCCCA 
CAGCAGGCCA GCACACTGAT GCAGCAGCTA CAGGAGGACT ATAACAGGCT TCTTACCAAG 
TATGCAGAAG CTGAAAATAC TATTGATCGA CTTCGCCTTG AAGCAAAGGT GGGTCTCTCT 
TCGGAACCAC CCAAACCCAG TAGTACAGCT GTCCTGTCGG GAGTTATTCA GGAAGGGTCA 
AAGGTCATAA CCATCAGTTT TCCTCAAGCA CAGAGGGCAG AGTTCAGTAC AGGTTCTGTT 
CATCTGACCC AACAGAAGGA GCTCTCAGAA AACTCCAGAA AAGCAGCAAC TCGGCCTTCT 
TCTGTAAGCT CTATCAGCTC GAGGAGGTCT GGATCTCTTA CTCCAGACCA TCTGACAGAC 
ACTCTGTCCA AACAGAACCA AAGATTTCAG CTACAGGTGG ATGCATTTGA GTCGCTTTTA 
AAGGGTGGGA AGCTCAAGCC ATGTGAGCAG ACAAAGGGTC TCTCCACACT GGCTGAGGGG 
CAGGAGTCTC TTGAGAGGGC TTATCTAAAT GCACGAGTCC AATATCAGAT GCTGCAGCAA 
CGACAAGGCA GACATGCCAT CTTTGACCCA GACAGGGATC TAGAGGGTCA GATCTTCAGG 
TCAGGGATGC GATTAGAGGA GCTGAAAGAG TGGCTGGAAC AAGCAGAACA GAACCAACCT 
GTTTCTGAAC CTACTTTATC CCCATATCCT CCTTCAGATG TCCTTTCTGC ATCCATGCTG 
GAGCCTGAAC CAGTGCCTGA GATCCCCCTG TCAGCAGTCT GTCCTGAGGC TTGTGTTGGT 
GTGGAGGTCA GTTCAGTCAG TGGAGAGAGT GATAGTGGTA GAGAGGAAAT GGAAATGCTT 
CCGTTTGTTC TTCATCCCCT CGATAACAAA CACCAACGTG TGGAGAAAGA TTTCAGTGAA 
CTCATGGACT GCTATCAGAG TGTTAAAGAG CTCCCCGGGA CACTGGATCA GGCTGTGACT 
TTAAAGAGCC ATGACTTGCT GGATTTTGGA AGCTCTGTTC CTCCTGGGAA AAAAAGTGTA 
ACGGACGGAC AACCACTCAA GACTGTTAAT GAGAGAGAGA TGAGAAAACC AGCTCAGCAG 
CAGCCGGCAG CAGGTTGTGT CCCTCCCTCA TGCCCATCTG TCAGTTCACA GGTGTGCGAC 
TCTATGGAAA CTCACCCAGA GGCTTTTTGC CACTCCTATG TATTGGCAGG AGAATCGACC 
AGAGAGAGTA AAACAGCAGG AAACATGAAA GCTCAAAGCA CTAGCTTGAC CAGCCTGGAA 
GAGAGAACAC ACCACAGAAC TGTAGGCCTG AAAGCGAAGG CGAAGACTCT AAGAGCGCCC 
CCTATGGATG GGGTCATGTC TCCAGAAACA GACAGTGGCT TTATGGGCTC TGAAAGCAGT 
CAGCTCACTG CTGCATTACA CAGTCCTCTC CACCAGAGGG CAGTAATAAG TTCCATCAGA 
CTGTCTGACC CTCACACAAA GGACACAGAG AGACCAGAGT CTGCTTCTCC TTCTGGGCAG 
CCTTTCATTG TCTCTCCTTC TCCATCCCAA CCTTCTCCAA ACACCACAAA AAAGCACATT 
GATACATCAG GAAAATGCAC AGGTCCTTAT AAGAAAATAG GAGGGGAGGA AAGGTGTTCT 
TTTTCCAGTC TTTCTTCCTC CTTATCCCCC CTTCACTGGC CACGTAGCAG CATTCTGCCC 
TGGACAAGCA GCCTGACCAG TCAATCAGAG CGTGGAGCTG ATGAGGAAGA AGAAAAAGCA 
CAGAAAGACA AATACCCACA ACCGACCAAT CAGCAACTCC GCTCTCACAG AAGCCCCTCC 
CTCCACAGGC CTTATCACCA CAGTTCTGCT CAGCTAACAA ATCACCAAAA GGCTCTTCAA 
TCTCTGCAAA AAGAAGTGAA CCGATTGAAA GAGAGATTAG AGGGAAATCT GAGACTATCA 
AAACCTGCCA GACCTATGAG AACAGAAGTT TCTGCCTCTG AAGACACCAG AGGTCACACA 
CATCCACAGA CATCAACACC TCAGAGGCAT GATTTAAGGT CTTCAAAGAG GAGGAGAAGG 
CAGACAGAGA GAGCTGGCAA TGGAGAAAAA GAGAGAGGAA TTTCACCTAG ACCAGTCCTC 
AGACAAAGAT CAGCATCTTT ACCTCGACAT CGGCCTGAAA CTGATATGAC ATCTGATTCT 
GAGCATGCTC AATCTGAGCC AAGACCATCA ACGTCTAGAT ATGTCCCAGT GTCGCCTGTG 
AAATCAAGAG GAAGAAGACA AACCAGGAGA TTTGAATGTC ACAAAGAGGA TAGTACATCA 
GGCAGATCTG ACAACAGCGA TGAAGCAGAA CCAAACGGAG GAGCAGAATC TCTGTGTCCT 
GATTGCATAT CCGATCGTAC CAGTGCTTTG ACACGTCTCC TGAGGAGCAG GAGCTCTCCA 
CATCTTGCAC AGACTTCCTG TAGCCATTGC CCATTGTGTG GTGCATTACA AGCCATTCAA 
AGAGCCTCTA AGCCAGCTCC AGTCTCCAGT CCCAGAGCGA GTCAGCCAAT GAGATCCTTA 
CAGAGAGAAA AAGGAGGCGT GAACGTGACA GTGGCTCCTC CCCCTTTATT GCAGAGAGCT 
GTTCCAGTTG TCCCATATGT GCCTGTATAT CCTTCAACTC TATATTTCTC CAGCCCAATG 
ACTCCACCGG CATACCCACA ACCCCTCAAC ATCTCATCAA ATCCCGTCAG GCCTGAGAGG 
TCAGGGGTCA GAGGTCACCG CAGACGCTCT TCCTCCATGG AGTATCATCA GGATTCTATG 
AGCAGCGCTT TGACCCGTGC CATCACTGCA GCGAGACACA TGAGAGAAGC CTCACAGCGC 
ATGGCCAGCA GCCTCCACAC TGCATCCTGC AGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

akna ( XM_009295258.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293533.1 Danio rerio AT-hook transcription factor (akna), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa25697
Clone ID Related Accession (Same CDS sequence)	XM_009295258.3 , XM_009295258.2 , XM_009295256.3 , XM_005161116.4 , XM_678843.6 , XM_005161116.3 , XM_009295257.2 , XM_678843.7 , XM_017353013.1 , XM_009295256.2 , XM_009295257.3
Accession Version	XM_009295258.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	AT-hook-containing transcription factor-like isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295258.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGGA GCAGAGGAGG CACCTCTACA GGGGTTCTGG TTTGGACACC TGCCCCACAG 
CTTTCCTCTC CCTCAGCCTC AGAGGGGTCA GACTTCAGCT GGGATGAAGA ACCGGACCAG 
GACTTCCAAA GCCAGATGGA TGAGAATGGG ATCATTGGTC TGGCAGAGAG GCAAGGGCAG 
CAGGAGAGAG AAGAGGAGGA GGTTGCAGAG GAGCTGTTGT GGGATGTGAA GACCCCTGAG 
CCTGAGCATG GGCCGCCGCC TGCAGTAGAG GAGATAAGCT GTCATCTGAG CGAACTGCTG 
GACTCTGAAC CGCTCTCTCA GGCCACTGGA GACCACTACT ACTCTCCATC ACTGCAGGAA 
GAACACCAAA CAGACGATGA TCGTAGTGTG TTGTCTGAGG ATTGGGCTGA TGACGAGGGT 
GGTACAGGGA GCCCAGTAAA GCACAAATTA AGCTCTTTCC CTGAGAGAGA CACTGCATTA 
GACATGACCG ATGAGGAGAC AGAAGAGAAA AACACACAAG AAGATGCTCA GAGATTCATG 
GATCAATCAT TTCATGTCAC AGAGGAGAGG GCAGCTGTGT TTCAAACTCT GAAAACCCTT 
GATTTCAGTC AGGAAATGAG TGGGATTTTC AAAGATCATC CAATTTCAGG AGTCCCAGAG 
TACTCTGATC AAAGTGACTG CGAGGAACTT GATGCCCGAT TTCTTCCAGT CTCGCATCAT 
TTCTCTAGAA ACACTTCCAA TCCCACCAGT TTTCCCCATT TATCCTCTGA GGAACTGATG 
AATGACTGTG GGATTGAAGC TGAGACGTTA CCTGAGCTAG AAATTAGGTC AGATTCATCT 
CTTGGCACTT ACCAGAGTCC TGTCTCTAAA ACAAACCACA CTTTCAGATC AAACCAGGAA 
GAGATTAAAC ACAAGATCTC ACCTCAAACT TCACCCAGAA GCCCTGTAAT AAAAAACAGC 
CCTGAAAAAA TATACCAAGA TGCTACGAGT GGCGAAAAAT TAGATGGAAG GTTACACAGG 
CCTTACCACA ATCTGCAGCC TCCAACAGTC TCTCCCAGAA AAACAACCTC TGCTTCCTGC 
AACACTCAGG TTTCCATTCC AGCGCAGGCT ATTTCTAAAG CAAGCGTCTC CCCCTCAGAG 
CGATCGCCTC CTCCTACACC TCACAGAAAT TCAGCAATAA AGAATCACAG AAACTGCAAA 
ACCTCTCACA TGGATCCAGA TGACATCAGG AAAGGACAGC TGAATCATGC CTTGCCTGAT 
TTCTCCAAAG TAGAGCCGAA GGTGCGTTTC CCAAAAAGAA GTGAATACAA ACCTCCCAAA 
AGCCGAAGAC CCCCTCATGT TCGAACATCA TACCCGGGTC TTCCAGTGGT GTTCAAATCT 
CCTGCTGAGA TTGTGAAAGA GGTCCTGTTG AGCAGCTGTG ATGGGACTAC TGGGAGTCCA 
TCCTCCAGCG AGACTCAGAA GCACCTGCAC AATATCGTCC CAGAAGAGTT TCGCTGCCCA 
CAGCAGGCCA GCACACTGAT GCAGCAGCTA CAGGAGGACT ATAACAGGCT TCTTACCAAG 
TATGCAGAAG CTGAAAATAC TATTGATCGA CTTCGCCTTG AAGCAAAGGT GGGTCTCTCT 
TCGGAACCAC CCAAACCCAG TAGTACAGCT GTCCTGTCGG GAGTTATTCA GGAAGGGTCA 
AAGGTCATAA CCATCAGTTT TCCTCAAGCA CAGAGGGCAG AGTTCAGTAC AGGTTCTGTT 
CATCTGACCC AACAGAAGGA GCTCTCAGAA AACTCCAGAA AAGCAGCAAC TCGGCCTTCT 
TCTGTAAGCT CTATCAGCTC GAGGAGGTCT GGATCTCTTA CTCCAGACCA TCTGACAGAC 
ACTCTGTCCA AACAGAACCA AAGATTTCAG CTACAGGTGG ATGCATTTGA GTCGCTTTTA 
AAGGGTGGGA AGCTCAAGCC ATGTGAGCAG ACAAAGGGTC TCTCCACACT GGCTGAGGGG 
CAGGAGTCTC TTGAGAGGGC TTATCTAAAT GCACGAGTCC AATATCAGAT GCTGCAGCAA 
CGACAAGGCA GACATGCCAT CTTTGACCCA GACAGGGATC TAGAGGGTCA GATCTTCAGG 
TCAGGGATGC GATTAGAGGA GCTGAAAGAG TGGCTGGAAC AAGCAGAACA GAACCAACCT 
GTTTCTGAAC CTACTTTATC CCCATATCCT CCTTCAGATG TCCTTTCTGC ATCCATGCTG 
GAGCCTGAAC CAGTGCCTGA GATCCCCCTG TCAGCAGTCT GTCCTGAGGC TTGTGTTGGT 
GTGGAGGTCA GTTCAGTCAG TGGAGAGAGT GATAGTGGTA GAGAGGAAAT GGAAATGCTT 
CCGTTTGTTC TTCATCCCCT CGATAACAAA CACCAACGTG TGGAGAAAGA TTTCAGTGAA 
CTCATGGACT GCTATCAGAG TGTTAAAGAG CTCCCCGGGA CACTGGATCA GGCTGTGACT 
TTAAAGAGCC ATGACTTGCT GGATTTTGGA AGCTCTGTTC CTCCTGGGAA AAAAAGTGTA 
ACGGACGGAC AACCACTCAA GACTGTTAAT GAGAGAGAGA TGAGAAAACC AGCTCAGCAG 
CAGCCGGCAG CAGGTTGTGT CCCTCCCTCA TGCCCATCTG TCAGTTCACA GGTGTGCGAC 
TCTATGGAAA CTCACCCAGA GGCTTTTTGC CACTCCTATG TATTGGCAGG AGAATCGACC 
AGAGAGAGTA AAACAGCAGG AAACATGAAA GCTCAAAGCA CTAGCTTGAC CAGCCTGGAA 
GAGAGAACAC ACCACAGAAC TGTAGGCCTG AAAGCGAAGG CGAAGACTCT AAGAGCGCCC 
CCTATGGATG GGGTCATGTC TCCAGAAACA GACAGTGGCT TTATGGGCTC TGAAAGCAGT 
CAGCTCACTG CTGCATTACA CAGTCCTCTC CACCAGAGGG CAGTAATAAG TTCCATCAGA 
CTGTCTGACC CTCACACAAA GGACACAGAG AGACCAGAGT CTGCTTCTCC TTCTGGGCAG 
CCTTTCATTG TCTCTCCTTC TCCATCCCAA CCTTCTCCAA ACACCACAAA AAAGCACATT 
GATACATCAG GAAAATGCAC AGGTCCTTAT AAGAAAATAG GAGGGGAGGA AAGGTGTTCT 
TTTTCCAGTC TTTCTTCCTC CTTATCCCCC CTTCACTGGC CACGTAGCAG CATTCTGCCC 
TGGACAAGCA GCCTGACCAG TCAATCAGAG CGTGGAGCTG ATGAGGAAGA AGAAAAAGCA 
CAGAAAGACA AATACCCACA ACCGACCAAT CAGCAACTCC GCTCTCACAG AAGCCCCTCC 
CTCCACAGGC CTTATCACCA CAGTTCTGCT CAGCTAACAA ATCACCAAAA GGCTCTTCAA 
TCTCTGCAAA AAGAAGTGAA CCGATTGAAA GAGAGATTAG AGGGAAATCT GAGACTATCA 
AAACCTGCCA GACCTATGAG AACAGAAGTT TCTGCCTCTG AAGACACCAG AGGTCACACA 
CATCCACAGA CATCAACACC TCAGAGGCAT GATTTAAGGT CTTCAAAGAG GAGGAGAAGG 
CAGACAGAGA GAGCTGGCAA TGGAGAAAAA GAGAGAGGAA TTTCACCTAG ACCAGTCCTC 
AGACAAAGAT CAGCATCTTT ACCTCGACAT CGGCCTGAAA CTGATATGAC ATCTGATTCT 
GAGCATGCTC AATCTGAGCC AAGACCATCA ACGTCTAGAT ATGTCCCAGT GTCGCCTGTG 
AAATCAAGAG GAAGAAGACA AACCAGGAGA TTTGAATGTC ACAAAGAGGA TAGTACATCA 
GGCAGATCTG ACAACAGCGA TGAAGCAGAA CCAAACGGAG GAGCAGAATC TCTGTGTCCT 
GATTGCATAT CCGATCGTAC CAGTGCTTTG ACACGTCTCC TGAGGAGCAG GAGCTCTCCA 
CATCTTGCAC AGACTTCCTG TAGCCATTGC CCATTGTGTG GTGCATTACA AGCCATTCAA 
AGAGCCTCTA AGCCAGCTCC AGTCTCCAGT CCCAGAGCGA GTCAGCCAAT GAGATCCTTA 
CAGAGAGAAA AAGGAGGCGT GAACGTGACA GTGGCTCCTC CCCCTTTATT GCAGAGAGCT 
GTTCCAGTTG TCCCATATGT GCCTGTATAT CCTTCAACTC TATATTTCTC CAGCCCAATG 
ACTCCACCGG CATACCCACA ACCCCTCAAC ATCTCATCAA ATCCCGTCAG GCCTGAGAGG 
TCAGGGGTCA GAGGTCACCG CAGACGCTCT TCCTCCATGG AGTATCATCA GGATTCTATG 
AGCAGCGCTT TGACCCGTGC CATCACTGCA GCGAGACACA TGAGAGAAGC CTCACAGCGC 
ATGGCCAGCA GCCTCCACAC TGCATCCTGC AGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293533.1
CDS	210..4568
Translation	MERSRGGTSTGVLVWTPAPQLSSPSASEGSDFSWDEEPDQDFQSQMDENGIIGLAERQGQQEREEEEVAEELLWDVKTPEPEHGPPPAVEEISCHLSELLDSEPLSQATGDHYYSPSLQEEHQTDDDRSVLSEDWADDEGGTGSPVKHKLSSFPERDTALDMTDEETEEKNTQEDAQRFMDQSFHVTEERAAVFQTLKTLDFSQEMSGIFKDHPISGVPEYSDQSDCEELDARFLPVSHHFSRNTSNPTSFPHLSSEELMNDCGIEAETLPELEIRSDSSLGTYQSPVSKTNHTFRSNQEEIKHKISPQTSPRSPVIKNSPEKIYQDATSGEKLDGRLHRPYHNLQPPTVSPRKTTSASCNTQVSIPAQAISKASVSPSERSPPPTPHRNSAIKNHRNCKTSHMDPDDIRKGQLNHALPDFSKVEPKVRFPKRSEYKPPKSRRPPHVRTSYPGLPVVFKSPAEIVKEVLLSSCDGTTGSPSSSETQKHLHNIVPEEFRCPQQASTLMQQLQEDYNRLLTKYAEAENTIDRLRLEAKVGLSSEPPKPSSTAVLSGVIQEGSKVITISFPQAQRAEFSTGSVHLTQQKELSENSRKAATRPSSVSSISSRRSGSLTPDHLTDTLSKQNQRFQLQVDAFESLLKGGKLKPCEQTKGLSTLAEGQESLERAYLNARVQYQMLQQRQGRHAIFDPDRDLEGQIFRSGMRLEELKEWLEQAEQNQPVSEPTLSPYPPSDVLSASMLEPEPVPEIPLSAVCPEACVGVEVSSVSGESDSGREEMEMLPFVLHPLDNKHQRVEKDFSELMDCYQSVKELPGTLDQAVTLKSHDLLDFGSSVPPGKKSVTDGQPLKTVNEREMRKPAQQQPAAGCVPPSCPSVSSQVCDSMETHPEAFCHSYVLAGESTRESKTAGNMKAQSTSLTSLEERTHHRTVGLKAKAKTLRAPPMDGVMSPETDSGFMGSESSQLTAALHSPLHQRAVISSIRLSDPHTKDTERPESASPSGQPFIVSPSPSQPSPNTTKKHIDTSGKCTGPYKKIGGEERCSFSSLSSSLSPLHWPRSSILPWTSSLTSQSERGADEEEEKAQKDKYPQPTNQQLRSHRSPSLHRPYHHSSAQLTNHQKALQSLQKEVNRLKERLEGNLRLSKPARPMRTEVSASEDTRGHTHPQTSTPQRHDLRSSKRRRRQTERAGNGEKERGISPRPVLRQRSASLPRHRPETDMTSDSEHAQSEPRPSTSRYVPVSPVKSRGRRQTRRFECHKEDSTSGRSDNSDEAEPNGGAESLCPDCISDRTSALTRLLRSRSSPHLAQTSCSHCPLCGALQAIQRASKPAPVSSPRASQPMRSLQREKGGVNVTVAPPPLLQRAVPVVPYVPVYPSTLYFSSPMTPPAYPQPLNISSNPVRPERSGVRGHRRRSSSMEYHQDSMSSALTRAITAARHMREASQRMASSLHTASCSY

Target ORF information:

RefSeq Version	XM_009295258.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio AT-hook transcription factor (akna), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295258.2

ORF Insert Sequence:

ATGGAGCGGA GCAGAGGAGG CACCTCTACA GGGGTTCTGG TTTGGACACC TGCCCCACAG 
CTTTCCTCTC CCTCAGCCTC AGAGGGGTCA GACTTCAGCT GGGATGAAGA ACCGGACCAG 
GACTTCCAAA GCCAGATGGA TGAGAATGGG ATCATTGGTC TGGCAGAGAG GCAAGGGCAG 
CAGGAGAGAG AAGAGGAGGA GGTTGCAGAG GAGCTGTTGT GGGATGTGAA GACCCCTGAG 
CCTGAGCATG GGCCGCCGCC TGCAGTAGAG GAGATAAGCT GTCATCTGAG CGAACTGCTG 
GACTCTGAAC CGCTCTCTCA GGCCACTGGA GACCACTACT ACTCTCCATC ACTGCAGGAA 
GAACACCAAA CAGACGATGA TCGTAGTGTG TTGTCTGAGG ATTGGGCTGA TGACGAGGGT 
GGTACAGGGA GCCCAGTAAA GCACAAATTA AGCTCTTTCC CTGAGAGAGA CACTGCATTA 
GACATGACCG ATGAGGAGAC AGAAGAGAAA AACACACAAG AAGATGCTCA GAGATTCATG 
GATCAATCAT TTCATGTCAC AGAGGAGAGG GCAGCTGTGT TTCAAACTCT GAAAACCCTT 
GATTTCAGTC AGGAAATGAG TGGGATTTTC AAAGATCATC CAATTTCAGG AGTCCCAGAG 
TACTCTGATC AAAGTGACTG CGAGGAACTT GATGCCCGAT TTCTTCCAGT CTCGCATCAT 
TTCTCTAGAA ACACTTCCAA TCCCACCAGT TTTCCCCATT TATCCTCTGA GGAACTGATG 
AATGACTGTG GGATTGAAGC TGAGACGTTA CCTGAGCTAG AAATTAGGTC AGATTCATCT 
CTTGGCACTT ACCAGAGTCC TGTCTCTAAA ACAAACCACA CTTTCAGATC AAACCAGGAA 
GAGATTAAAC ACAAGATCTC ACCTCAAACT TCACCCAGAA GCCCTGTAAT AAAAAACAGC 
CCTGAAAAAA TATACCAAGA TGCTACGAGT GGCGAAAAAT TAGATGGAAG GTTACACAGG 
CCTTACCACA ATCTGCAGCC TCCAACAGTC TCTCCCAGAA AAACAACCTC TGCTTCCTGC 
AACACTCAGG TTTCCATTCC AGCGCAGGCT ATTTCTAAAG CAAGCGTCTC CCCCTCAGAG 
CGATCGCCTC CTCCTACACC TCACAGAAAT TCAGCAATAA AGAATCACAG AAACTGCAAA 
ACCTCTCACA TGGATCCAGA TGACATCAGG AAAGGACAGC TGAATCATGC CTTGCCTGAT 
TTCTCCAAAG TAGAGCCGAA GGTGCGTTTC CCAAAAAGAA GTGAATACAA ACCTCCCAAA 
AGCCGAAGAC CCCCTCATGT TCGAACATCA TACCCGGGTC TTCCAGTGGT GTTCAAATCT 
CCTGCTGAGA TTGTGAAAGA GGTCCTGTTG AGCAGCTGTG ATGGGACTAC TGGGAGTCCA 
TCCTCCAGCG AGACTCAGAA GCACCTGCAC AATATCGTCC CAGAAGAGTT TCGCTGCCCA 
CAGCAGGCCA GCACACTGAT GCAGCAGCTA CAGGAGGACT ATAACAGGCT TCTTACCAAG 
TATGCAGAAG CTGAAAATAC TATTGATCGA CTTCGCCTTG AAGCAAAGGT GGGTCTCTCT 
TCGGAACCAC CCAAACCCAG TAGTACAGCT GTCCTGTCGG GAGTTATTCA GGAAGGGTCA 
AAGGTCATAA CCATCAGTTT TCCTCAAGCA CAGAGGGCAG AGTTCAGTAC AGGTTCTGTT 
CATCTGACCC AACAGAAGGA GCTCTCAGAA AACTCCAGAA AAGCAGCAAC TCGGCCTTCT 
TCTGTAAGCT CTATCAGCTC GAGGAGGTCT GGATCTCTTA CTCCAGACCA TCTGACAGAC 
ACTCTGTCCA AACAGAACCA AAGATTTCAG CTACAGGTGG ATGCATTTGA GTCGCTTTTA 
AAGGGTGGGA AGCTCAAGCC ATGTGAGCAG ACAAAGGGTC TCTCCACACT GGCTGAGGGG 
CAGGAGTCTC TTGAGAGGGC TTATCTAAAT GCACGAGTCC AATATCAGAT GCTGCAGCAA 
CGACAAGGCA GACATGCCAT CTTTGACCCA GACAGGGATC TAGAGGGTCA GATCTTCAGG 
TCAGGGATGC GATTAGAGGA GCTGAAAGAG TGGCTGGAAC AAGCAGAACA GAACCAACCT 
GTTTCTGAAC CTACTTTATC CCCATATCCT CCTTCAGATG TCCTTTCTGC ATCCATGCTG 
GAGCCTGAAC CAGTGCCTGA GATCCCCCTG TCAGCAGTCT GTCCTGAGGC TTGTGTTGGT 
GTGGAGGTCA GTTCAGTCAG TGGAGAGAGT GATAGTGGTA GAGAGGAAAT GGAAATGCTT 
CCGTTTGTTC TTCATCCCCT CGATAACAAA CACCAACGTG TGGAGAAAGA TTTCAGTGAA 
CTCATGGACT GCTATCAGAG TGTTAAAGAG CTCCCCGGGA CACTGGATCA GGCTGTGACT 
TTAAAGAGCC ATGACTTGCT GGATTTTGGA AGCTCTGTTC CTCCTGGGAA AAAAAGTGTA 
ACGGACGGAC AACCACTCAA GACTGTTAAT GAGAGAGAGA TGAGAAAACC AGCTCAGCAG 
CAGCCGGCAG CAGGTTGTGT CCCTCCCTCA TGCCCATCTG TCAGTTCACA GGTGTGCGAC 
TCTATGGAAA CTCACCCAGA GGCTTTTTGC CACTCCTATG TATTGGCAGG AGAATCGACC 
AGAGAGAGTA AAACAGCAGG AAACATGAAA GCTCAAAGCA CTAGCTTGAC CAGCCTGGAA 
GAGAGAACAC ACCACAGAAC TGTAGGCCTG AAAGCGAAGG CGAAGACTCT AAGAGCGCCC 
CCTATGGATG GGGTCATGTC TCCAGAAACA GACAGTGGCT TTATGGGCTC TGAAAGCAGT 
CAGCTCACTG CTGCATTACA CAGTCCTCTC CACCAGAGGG CAGTAATAAG TTCCATCAGA 
CTGTCTGACC CTCACACAAA GGACACAGAG AGACCAGAGT CTGCTTCTCC TTCTGGGCAG 
CCTTTCATTG TCTCTCCTTC TCCATCCCAA CCTTCTCCAA ACACCACAAA AAAGCACATT 
GATACATCAG GAAAATGCAC AGGTCCTTAT AAGAAAATAG GAGGGGAGGA AAGGTGTTCT 
TTTTCCAGTC TTTCTTCCTC CTTATCCCCC CTTCACTGGC CACGTAGCAG CATTCTGCCC 
TGGACAAGCA GCCTGACCAG TCAATCAGAG CGTGGAGCTG ATGAGGAAGA AGAAAAAGCA 
CAGAAAGACA AATACCCACA ACCGACCAAT CAGCAACTCC GCTCTCACAG AAGCCCCTCC 
CTCCACAGGC CTTATCACCA CAGTTCTGCT CAGCTAACAA ATCACCAAAA GGCTCTTCAA 
TCTCTGCAAA AAGAAGTGAA CCGATTGAAA GAGAGATTAG AGGGAAATCT GAGACTATCA 
AAACCTGCCA GACCTATGAG AACAGAAGTT TCTGCCTCTG AAGACACCAG AGGTCACACA 
CATCCACAGA CATCAACACC TCAGAGGCAT GATTTAAGGT CTTCAAAGAG GAGGAGAAGG 
CAGACAGAGA GAGCTGGCAA TGGAGAAAAA GAGAGAGGAA TTTCACCTAG ACCAGTCCTC 
AGACAAAGAT CAGCATCTTT ACCTCGACAT CGGCCTGAAA CTGATATGAC ATCTGATTCT 
GAGCATGCTC AATCTGAGCC AAGACCATCA ACGTCTAGAT ATGTCCCAGT GTCGCCTGTG 
AAATCAAGAG GAAGAAGACA AACCAGGAGA TTTGAATGTC ACAAAGAGGA TAGTACATCA 
GGCAGATCTG ACAACAGCGA TGAAGCAGAA CCAAACGGAG GAGCAGAATC TCTGTGTCCT 
GATTGCATAT CCGATCGTAC CAGTGCTTTG ACACGTCTCC TGAGGAGCAG GAGCTCTCCA 
CATCTTGCAC AGACTTCCTG TAGCCATTGC CCATTGTGTG GTGCATTACA AGCCATTCAA 
AGAGCCTCTA AGCCAGCTCC AGTCTCCAGT CCCAGAGCGA GTCAGCCAAT GAGATCCTTA 
CAGAGAGAAA AAGGAGGCGT GAACGTGACA GTGGCTCCTC CCCCTTTATT GCAGAGAGCT 
GTTCCAGTTG TCCCATATGT GCCTGTATAT CCTTCAACTC TATATTTCTC CAGCCCAATG 
ACTCCACCGG CATACCCACA ACCCCTCAAC ATCTCATCAA ATCCCGTCAG GCCTGAGAGG 
TCAGGGGTCA GAGGTCACCG CAGACGCTCT TCCTCCATGG AGTATCATCA GGATTCTATG 
AGCAGCGCTT TGACCCGTGC CATCACTGCA GCGAGACACA TGAGAGAAGC CTCACAGCGC 
ATGGCCAGCA GCCTCCACAC TGCATCCTGC AGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

akna ( XM_009295256.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293531.1 Danio rerio AT-hook transcription factor (akna), transcript variant X2, mRNA.

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CloneID	ODa25697
Clone ID Related Accession (Same CDS sequence)	XM_009295258.3 , XM_009295258.2 , XM_009295256.3 , XM_005161116.4 , XM_678843.6 , XM_005161116.3 , XM_009295257.2 , XM_678843.7 , XM_017353013.1 , XM_009295256.2 , XM_009295257.3
Accession Version	XM_009295256.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	AT-hook-containing transcription factor-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009295256.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGGA GCAGAGGAGG CACCTCTACA GGGGTTCTGG TTTGGACACC TGCCCCACAG 
CTTTCCTCTC CCTCAGCCTC AGAGGGGTCA GACTTCAGCT GGGATGAAGA ACCGGACCAG 
GACTTCCAAA GCCAGATGGA TGAGAATGGG ATCATTGGTC TGGCAGAGAG GCAAGGGCAG 
CAGGAGAGAG AAGAGGAGGA GGTTGCAGAG GAGCTGTTGT GGGATGTGAA GACCCCTGAG 
CCTGAGCATG GGCCGCCGCC TGCAGTAGAG GAGATAAGCT GTCATCTGAG CGAACTGCTG 
GACTCTGAAC CGCTCTCTCA GGCCACTGGA GACCACTACT ACTCTCCATC ACTGCAGGAA 
GAACACCAAA CAGACGATGA TCGTAGTGTG TTGTCTGAGG ATTGGGCTGA TGACGAGGGT 
GGTACAGGGA GCCCAGTAAA GCACAAATTA AGCTCTTTCC CTGAGAGAGA CACTGCATTA 
GACATGACCG ATGAGGAGAC AGAAGAGAAA AACACACAAG AAGATGCTCA GAGATTCATG 
GATCAATCAT TTCATGTCAC AGAGGAGAGG GCAGCTGTGT TTCAAACTCT GAAAACCCTT 
GATTTCAGTC AGGAAATGAG TGGGATTTTC AAAGATCATC CAATTTCAGG AGTCCCAGAG 
TACTCTGATC AAAGTGACTG CGAGGAACTT GATGCCCGAT TTCTTCCAGT CTCGCATCAT 
TTCTCTAGAA ACACTTCCAA TCCCACCAGT TTTCCCCATT TATCCTCTGA GGAACTGATG 
AATGACTGTG GGATTGAAGC TGAGACGTTA CCTGAGCTAG AAATTAGGTC AGATTCATCT 
CTTGGCACTT ACCAGAGTCC TGTCTCTAAA ACAAACCACA CTTTCAGATC AAACCAGGAA 
GAGATTAAAC ACAAGATCTC ACCTCAAACT TCACCCAGAA GCCCTGTAAT AAAAAACAGC 
CCTGAAAAAA TATACCAAGA TGCTACGAGT GGCGAAAAAT TAGATGGAAG GTTACACAGG 
CCTTACCACA ATCTGCAGCC TCCAACAGTC TCTCCCAGAA AAACAACCTC TGCTTCCTGC 
AACACTCAGG TTTCCATTCC AGCGCAGGCT ATTTCTAAAG CAAGCGTCTC CCCCTCAGAG 
CGATCGCCTC CTCCTACACC TCACAGAAAT TCAGCAATAA AGAATCACAG AAACTGCAAA 
ACCTCTCACA TGGATCCAGA TGACATCAGG AAAGGACAGC TGAATCATGC CTTGCCTGAT 
TTCTCCAAAG TAGAGCCGAA GGTGCGTTTC CCAAAAAGAA GTGAATACAA ACCTCCCAAA 
AGCCGAAGAC CCCCTCATGT TCGAACATCA TACCCGGGTC TTCCAGTGGT GTTCAAATCT 
CCTGCTGAGA TTGTGAAAGA GGTCCTGTTG AGCAGCTGTG ATGGGACTAC TGGGAGTCCA 
TCCTCCAGCG AGACTCAGAA GCACCTGCAC AATATCGTCC CAGAAGAGTT TCGCTGCCCA 
CAGCAGGCCA GCACACTGAT GCAGCAGCTA CAGGAGGACT ATAACAGGCT TCTTACCAAG 
TATGCAGAAG CTGAAAATAC TATTGATCGA CTTCGCCTTG AAGCAAAGGT GGGTCTCTCT 
TCGGAACCAC CCAAACCCAG TAGTACAGCT GTCCTGTCGG GAGTTATTCA GGAAGGGTCA 
AAGGTCATAA CCATCAGTTT TCCTCAAGCA CAGAGGGCAG AGTTCAGTAC AGGTTCTGTT 
CATCTGACCC AACAGAAGGA GCTCTCAGAA AACTCCAGAA AAGCAGCAAC TCGGCCTTCT 
TCTGTAAGCT CTATCAGCTC GAGGAGGTCT GGATCTCTTA CTCCAGACCA TCTGACAGAC 
ACTCTGTCCA AACAGAACCA AAGATTTCAG CTACAGGTGG ATGCATTTGA GTCGCTTTTA 
AAGGGTGGGA AGCTCAAGCC ATGTGAGCAG ACAAAGGGTC TCTCCACACT GGCTGAGGGG 
CAGGAGTCTC TTGAGAGGGC TTATCTAAAT GCACGAGTCC AATATCAGAT GCTGCAGCAA 
CGACAAGGCA GACATGCCAT CTTTGACCCA GACAGGGATC TAGAGGGTCA GATCTTCAGG 
TCAGGGATGC GATTAGAGGA GCTGAAAGAG TGGCTGGAAC AAGCAGAACA GAACCAACCT 
GTTTCTGAAC CTACTTTATC CCCATATCCT CCTTCAGATG TCCTTTCTGC ATCCATGCTG 
GAGCCTGAAC CAGTGCCTGA GATCCCCCTG TCAGCAGTCT GTCCTGAGGC TTGTGTTGGT 
GTGGAGGTCA GTTCAGTCAG TGGAGAGAGT GATAGTGGTA GAGAGGAAAT GGAAATGCTT 
CCGTTTGTTC TTCATCCCCT CGATAACAAA CACCAACGTG TGGAGAAAGA TTTCAGTGAA 
CTCATGGACT GCTATCAGAG TGTTAAAGAG CTCCCCGGGA CACTGGATCA GGCTGTGACT 
TTAAAGAGCC ATGACTTGCT GGATTTTGGA AGCTCTGTTC CTCCTGGGAA AAAAAGTGTA 
ACGGACGGAC AACCACTCAA GACTGTTAAT GAGAGAGAGA TGAGAAAACC AGCTCAGCAG 
CAGCCGGCAG CAGGTTGTGT CCCTCCCTCA TGCCCATCTG TCAGTTCACA GGTGTGCGAC 
TCTATGGAAA CTCACCCAGA GGCTTTTTGC CACTCCTATG TATTGGCAGG AGAATCGACC 
AGAGAGAGTA AAACAGCAGG AAACATGAAA GCTCAAAGCA CTAGCTTGAC CAGCCTGGAA 
GAGAGAACAC ACCACAGAAC TGTAGGCCTG AAAGCGAAGG CGAAGACTCT AAGAGCGCCC 
CCTATGGATG GGGTCATGTC TCCAGAAACA GACAGTGGCT TTATGGGCTC TGAAAGCAGT 
CAGCTCACTG CTGCATTACA CAGTCCTCTC CACCAGAGGG CAGTAATAAG TTCCATCAGA 
CTGTCTGACC CTCACACAAA GGACACAGAG AGACCAGAGT CTGCTTCTCC TTCTGGGCAG 
CCTTTCATTG TCTCTCCTTC TCCATCCCAA CCTTCTCCAA ACACCACAAA AAAGCACATT 
GATACATCAG GAAAATGCAC AGGTCCTTAT AAGAAAATAG GAGGGGAGGA AAGGTGTTCT 
TTTTCCAGTC TTTCTTCCTC CTTATCCCCC CTTCACTGGC CACGTAGCAG CATTCTGCCC 
TGGACAAGCA GCCTGACCAG TCAATCAGAG CGTGGAGCTG ATGAGGAAGA AGAAAAAGCA 
CAGAAAGACA AATACCCACA ACCGACCAAT CAGCAACTCC GCTCTCACAG AAGCCCCTCC 
CTCCACAGGC CTTATCACCA CAGTTCTGCT CAGCTAACAA ATCACCAAAA GGCTCTTCAA 
TCTCTGCAAA AAGAAGTGAA CCGATTGAAA GAGAGATTAG AGGGAAATCT GAGACTATCA 
AAACCTGCCA GACCTATGAG AACAGAAGTT TCTGCCTCTG AAGACACCAG AGGTCACACA 
CATCCACAGA CATCAACACC TCAGAGGCAT GATTTAAGGT CTTCAAAGAG GAGGAGAAGG 
CAGACAGAGA GAGCTGGCAA TGGAGAAAAA GAGAGAGGAA TTTCACCTAG ACCAGTCCTC 
AGACAAAGAT CAGCATCTTT ACCTCGACAT CGGCCTGAAA CTGATATGAC ATCTGATTCT 
GAGCATGCTC AATCTGAGCC AAGACCATCA ACGTCTAGAT ATGTCCCAGT GTCGCCTGTG 
AAATCAAGAG GAAGAAGACA AACCAGGAGA TTTGAATGTC ACAAAGAGGA TAGTACATCA 
GGCAGATCTG ACAACAGCGA TGAAGCAGAA CCAAACGGAG GAGCAGAATC TCTGTGTCCT 
GATTGCATAT CCGATCGTAC CAGTGCTTTG ACACGTCTCC TGAGGAGCAG GAGCTCTCCA 
CATCTTGCAC AGACTTCCTG TAGCCATTGC CCATTGTGTG GTGCATTACA AGCCATTCAA 
AGAGCCTCTA AGCCAGCTCC AGTCTCCAGT CCCAGAGCGA GTCAGCCAAT GAGATCCTTA 
CAGAGAGAAA AAGGAGGCGT GAACGTGACA GTGGCTCCTC CCCCTTTATT GCAGAGAGCT 
GTTCCAGTTG TCCCATATGT GCCTGTATAT CCTTCAACTC TATATTTCTC CAGCCCAATG 
ACTCCACCGG CATACCCACA ACCCCTCAAC ATCTCATCAA ATCCCGTCAG GCCTGAGAGG 
TCAGGGGTCA GAGGTCACCG CAGACGCTCT TCCTCCATGG AGTATCATCA GGATTCTATG 
AGCAGCGCTT TGACCCGTGC CATCACTGCA GCGAGACACA TGAGAGAAGC CTCACAGCGC 
ATGGCCAGCA GCCTCCACAC TGCATCCTGC AGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293531.1
CDS	487..4845
Translation	MERSRGGTSTGVLVWTPAPQLSSPSASEGSDFSWDEEPDQDFQSQMDENGIIGLAERQGQQEREEEEVAEELLWDVKTPEPEHGPPPAVEEISCHLSELLDSEPLSQATGDHYYSPSLQEEHQTDDDRSVLSEDWADDEGGTGSPVKHKLSSFPERDTALDMTDEETEEKNTQEDAQRFMDQSFHVTEERAAVFQTLKTLDFSQEMSGIFKDHPISGVPEYSDQSDCEELDARFLPVSHHFSRNTSNPTSFPHLSSEELMNDCGIEAETLPELEIRSDSSLGTYQSPVSKTNHTFRSNQEEIKHKISPQTSPRSPVIKNSPEKIYQDATSGEKLDGRLHRPYHNLQPPTVSPRKTTSASCNTQVSIPAQAISKASVSPSERSPPPTPHRNSAIKNHRNCKTSHMDPDDIRKGQLNHALPDFSKVEPKVRFPKRSEYKPPKSRRPPHVRTSYPGLPVVFKSPAEIVKEVLLSSCDGTTGSPSSSETQKHLHNIVPEEFRCPQQASTLMQQLQEDYNRLLTKYAEAENTIDRLRLEAKVGLSSEPPKPSSTAVLSGVIQEGSKVITISFPQAQRAEFSTGSVHLTQQKELSENSRKAATRPSSVSSISSRRSGSLTPDHLTDTLSKQNQRFQLQVDAFESLLKGGKLKPCEQTKGLSTLAEGQESLERAYLNARVQYQMLQQRQGRHAIFDPDRDLEGQIFRSGMRLEELKEWLEQAEQNQPVSEPTLSPYPPSDVLSASMLEPEPVPEIPLSAVCPEACVGVEVSSVSGESDSGREEMEMLPFVLHPLDNKHQRVEKDFSELMDCYQSVKELPGTLDQAVTLKSHDLLDFGSSVPPGKKSVTDGQPLKTVNEREMRKPAQQQPAAGCVPPSCPSVSSQVCDSMETHPEAFCHSYVLAGESTRESKTAGNMKAQSTSLTSLEERTHHRTVGLKAKAKTLRAPPMDGVMSPETDSGFMGSESSQLTAALHSPLHQRAVISSIRLSDPHTKDTERPESASPSGQPFIVSPSPSQPSPNTTKKHIDTSGKCTGPYKKIGGEERCSFSSLSSSLSPLHWPRSSILPWTSSLTSQSERGADEEEEKAQKDKYPQPTNQQLRSHRSPSLHRPYHHSSAQLTNHQKALQSLQKEVNRLKERLEGNLRLSKPARPMRTEVSASEDTRGHTHPQTSTPQRHDLRSSKRRRRQTERAGNGEKERGISPRPVLRQRSASLPRHRPETDMTSDSEHAQSEPRPSTSRYVPVSPVKSRGRRQTRRFECHKEDSTSGRSDNSDEAEPNGGAESLCPDCISDRTSALTRLLRSRSSPHLAQTSCSHCPLCGALQAIQRASKPAPVSSPRASQPMRSLQREKGGVNVTVAPPPLLQRAVPVVPYVPVYPSTLYFSSPMTPPAYPQPLNISSNPVRPERSGVRGHRRRSSSMEYHQDSMSSALTRAITAARHMREASQRMASSLHTASCSY

Target ORF information:

RefSeq Version	XM_009295256.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio AT-hook transcription factor (akna), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295256.3

ORF Insert Sequence:

ATGGAGCGGA GCAGAGGAGG CACCTCTACA GGGGTTCTGG TTTGGACACC TGCCCCACAG 
CTTTCCTCTC CCTCAGCCTC AGAGGGGTCA GACTTCAGCT GGGATGAAGA ACCGGACCAG 
GACTTCCAAA GCCAGATGGA TGAGAATGGG ATCATTGGTC TGGCAGAGAG GCAAGGGCAG 
CAGGAGAGAG AAGAGGAGGA GGTTGCAGAG GAGCTGTTGT GGGATGTGAA GACCCCTGAG 
CCTGAGCATG GGCCGCCGCC TGCAGTAGAG GAGATAAGCT GTCATCTGAG CGAACTGCTG 
GACTCTGAAC CGCTCTCTCA GGCCACTGGA GACCACTACT ACTCTCCATC ACTGCAGGAA 
GAACACCAAA CAGACGATGA TCGTAGTGTG TTGTCTGAGG ATTGGGCTGA TGACGAGGGT 
GGTACAGGGA GCCCAGTAAA GCACAAATTA AGCTCTTTCC CTGAGAGAGA CACTGCATTA 
GACATGACCG ATGAGGAGAC AGAAGAGAAA AACACACAAG AAGATGCTCA GAGATTCATG 
GATCAATCAT TTCATGTCAC AGAGGAGAGG GCAGCTGTGT TTCAAACTCT GAAAACCCTT 
GATTTCAGTC AGGAAATGAG TGGGATTTTC AAAGATCATC CAATTTCAGG AGTCCCAGAG 
TACTCTGATC AAAGTGACTG CGAGGAACTT GATGCCCGAT TTCTTCCAGT CTCGCATCAT 
TTCTCTAGAA ACACTTCCAA TCCCACCAGT TTTCCCCATT TATCCTCTGA GGAACTGATG 
AATGACTGTG GGATTGAAGC TGAGACGTTA CCTGAGCTAG AAATTAGGTC AGATTCATCT 
CTTGGCACTT ACCAGAGTCC TGTCTCTAAA ACAAACCACA CTTTCAGATC AAACCAGGAA 
GAGATTAAAC ACAAGATCTC ACCTCAAACT TCACCCAGAA GCCCTGTAAT AAAAAACAGC 
CCTGAAAAAA TATACCAAGA TGCTACGAGT GGCGAAAAAT TAGATGGAAG GTTACACAGG 
CCTTACCACA ATCTGCAGCC TCCAACAGTC TCTCCCAGAA AAACAACCTC TGCTTCCTGC 
AACACTCAGG TTTCCATTCC AGCGCAGGCT ATTTCTAAAG CAAGCGTCTC CCCCTCAGAG 
CGATCGCCTC CTCCTACACC TCACAGAAAT TCAGCAATAA AGAATCACAG AAACTGCAAA 
ACCTCTCACA TGGATCCAGA TGACATCAGG AAAGGACAGC TGAATCATGC CTTGCCTGAT 
TTCTCCAAAG TAGAGCCGAA GGTGCGTTTC CCAAAAAGAA GTGAATACAA ACCTCCCAAA 
AGCCGAAGAC CCCCTCATGT TCGAACATCA TACCCGGGTC TTCCAGTGGT GTTCAAATCT 
CCTGCTGAGA TTGTGAAAGA GGTCCTGTTG AGCAGCTGTG ATGGGACTAC TGGGAGTCCA 
TCCTCCAGCG AGACTCAGAA GCACCTGCAC AATATCGTCC CAGAAGAGTT TCGCTGCCCA 
CAGCAGGCCA GCACACTGAT GCAGCAGCTA CAGGAGGACT ATAACAGGCT TCTTACCAAG 
TATGCAGAAG CTGAAAATAC TATTGATCGA CTTCGCCTTG AAGCAAAGGT GGGTCTCTCT 
TCGGAACCAC CCAAACCCAG TAGTACAGCT GTCCTGTCGG GAGTTATTCA GGAAGGGTCA 
AAGGTCATAA CCATCAGTTT TCCTCAAGCA CAGAGGGCAG AGTTCAGTAC AGGTTCTGTT 
CATCTGACCC AACAGAAGGA GCTCTCAGAA AACTCCAGAA AAGCAGCAAC TCGGCCTTCT 
TCTGTAAGCT CTATCAGCTC GAGGAGGTCT GGATCTCTTA CTCCAGACCA TCTGACAGAC 
ACTCTGTCCA AACAGAACCA AAGATTTCAG CTACAGGTGG ATGCATTTGA GTCGCTTTTA 
AAGGGTGGGA AGCTCAAGCC ATGTGAGCAG ACAAAGGGTC TCTCCACACT GGCTGAGGGG 
CAGGAGTCTC TTGAGAGGGC TTATCTAAAT GCACGAGTCC AATATCAGAT GCTGCAGCAA 
CGACAAGGCA GACATGCCAT CTTTGACCCA GACAGGGATC TAGAGGGTCA GATCTTCAGG 
TCAGGGATGC GATTAGAGGA GCTGAAAGAG TGGCTGGAAC AAGCAGAACA GAACCAACCT 
GTTTCTGAAC CTACTTTATC CCCATATCCT CCTTCAGATG TCCTTTCTGC ATCCATGCTG 
GAGCCTGAAC CAGTGCCTGA GATCCCCCTG TCAGCAGTCT GTCCTGAGGC TTGTGTTGGT 
GTGGAGGTCA GTTCAGTCAG TGGAGAGAGT GATAGTGGTA GAGAGGAAAT GGAAATGCTT 
CCGTTTGTTC TTCATCCCCT CGATAACAAA CACCAACGTG TGGAGAAAGA TTTCAGTGAA 
CTCATGGACT GCTATCAGAG TGTTAAAGAG CTCCCCGGGA CACTGGATCA GGCTGTGACT 
TTAAAGAGCC ATGACTTGCT GGATTTTGGA AGCTCTGTTC CTCCTGGGAA AAAAAGTGTA 
ACGGACGGAC AACCACTCAA GACTGTTAAT GAGAGAGAGA TGAGAAAACC AGCTCAGCAG 
CAGCCGGCAG CAGGTTGTGT CCCTCCCTCA TGCCCATCTG TCAGTTCACA GGTGTGCGAC 
TCTATGGAAA CTCACCCAGA GGCTTTTTGC CACTCCTATG TATTGGCAGG AGAATCGACC 
AGAGAGAGTA AAACAGCAGG AAACATGAAA GCTCAAAGCA CTAGCTTGAC CAGCCTGGAA 
GAGAGAACAC ACCACAGAAC TGTAGGCCTG AAAGCGAAGG CGAAGACTCT AAGAGCGCCC 
CCTATGGATG GGGTCATGTC TCCAGAAACA GACAGTGGCT TTATGGGCTC TGAAAGCAGT 
CAGCTCACTG CTGCATTACA CAGTCCTCTC CACCAGAGGG CAGTAATAAG TTCCATCAGA 
CTGTCTGACC CTCACACAAA GGACACAGAG AGACCAGAGT CTGCTTCTCC TTCTGGGCAG 
CCTTTCATTG TCTCTCCTTC TCCATCCCAA CCTTCTCCAA ACACCACAAA AAAGCACATT 
GATACATCAG GAAAATGCAC AGGTCCTTAT AAGAAAATAG GAGGGGAGGA AAGGTGTTCT 
TTTTCCAGTC TTTCTTCCTC CTTATCCCCC CTTCACTGGC CACGTAGCAG CATTCTGCCC 
TGGACAAGCA GCCTGACCAG TCAATCAGAG CGTGGAGCTG ATGAGGAAGA AGAAAAAGCA 
CAGAAAGACA AATACCCACA ACCGACCAAT CAGCAACTCC GCTCTCACAG AAGCCCCTCC 
CTCCACAGGC CTTATCACCA CAGTTCTGCT CAGCTAACAA ATCACCAAAA GGCTCTTCAA 
TCTCTGCAAA AAGAAGTGAA CCGATTGAAA GAGAGATTAG AGGGAAATCT GAGACTATCA 
AAACCTGCCA GACCTATGAG AACAGAAGTT TCTGCCTCTG AAGACACCAG AGGTCACACA 
CATCCACAGA CATCAACACC TCAGAGGCAT GATTTAAGGT CTTCAAAGAG GAGGAGAAGG 
CAGACAGAGA GAGCTGGCAA TGGAGAAAAA GAGAGAGGAA TTTCACCTAG ACCAGTCCTC 
AGACAAAGAT CAGCATCTTT ACCTCGACAT CGGCCTGAAA CTGATATGAC ATCTGATTCT 
GAGCATGCTC AATCTGAGCC AAGACCATCA ACGTCTAGAT ATGTCCCAGT GTCGCCTGTG 
AAATCAAGAG GAAGAAGACA AACCAGGAGA TTTGAATGTC ACAAAGAGGA TAGTACATCA 
GGCAGATCTG ACAACAGCGA TGAAGCAGAA CCAAACGGAG GAGCAGAATC TCTGTGTCCT 
GATTGCATAT CCGATCGTAC CAGTGCTTTG ACACGTCTCC TGAGGAGCAG GAGCTCTCCA 
CATCTTGCAC AGACTTCCTG TAGCCATTGC CCATTGTGTG GTGCATTACA AGCCATTCAA 
AGAGCCTCTA AGCCAGCTCC AGTCTCCAGT CCCAGAGCGA GTCAGCCAAT GAGATCCTTA 
CAGAGAGAAA AAGGAGGCGT GAACGTGACA GTGGCTCCTC CCCCTTTATT GCAGAGAGCT 
GTTCCAGTTG TCCCATATGT GCCTGTATAT CCTTCAACTC TATATTTCTC CAGCCCAATG 
ACTCCACCGG CATACCCACA ACCCCTCAAC ATCTCATCAA ATCCCGTCAG GCCTGAGAGG 
TCAGGGGTCA GAGGTCACCG CAGACGCTCT TCCTCCATGG AGTATCATCA GGATTCTATG 
AGCAGCGCTT TGACCCGTGC CATCACTGCA GCGAGACACA TGAGAGAAGC CTCACAGCGC 
ATGGCCAGCA GCCTCCACAC TGCATCCTGC AGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

akna ( XM_005161116.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161173.1 Danio rerio AT-hook transcription factor (akna), transcript variant X3, mRNA.

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CloneID	ODa25697
Clone ID Related Accession (Same CDS sequence)	XM_009295258.3 , XM_009295258.2 , XM_009295256.3 , XM_005161116.4 , XM_678843.6 , XM_005161116.3 , XM_009295257.2 , XM_678843.7 , XM_017353013.1 , XM_009295256.2 , XM_009295257.3
Accession Version	XM_005161116.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	AT-hook-containing transcription factor-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005161116.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGGA GCAGAGGAGG CACCTCTACA GGGGTTCTGG TTTGGACACC TGCCCCACAG 
CTTTCCTCTC CCTCAGCCTC AGAGGGGTCA GACTTCAGCT GGGATGAAGA ACCGGACCAG 
GACTTCCAAA GCCAGATGGA TGAGAATGGG ATCATTGGTC TGGCAGAGAG GCAAGGGCAG 
CAGGAGAGAG AAGAGGAGGA GGTTGCAGAG GAGCTGTTGT GGGATGTGAA GACCCCTGAG 
CCTGAGCATG GGCCGCCGCC TGCAGTAGAG GAGATAAGCT GTCATCTGAG CGAACTGCTG 
GACTCTGAAC CGCTCTCTCA GGCCACTGGA GACCACTACT ACTCTCCATC ACTGCAGGAA 
GAACACCAAA CAGACGATGA TCGTAGTGTG TTGTCTGAGG ATTGGGCTGA TGACGAGGGT 
GGTACAGGGA GCCCAGTAAA GCACAAATTA AGCTCTTTCC CTGAGAGAGA CACTGCATTA 
GACATGACCG ATGAGGAGAC AGAAGAGAAA AACACACAAG AAGATGCTCA GAGATTCATG 
GATCAATCAT TTCATGTCAC AGAGGAGAGG GCAGCTGTGT TTCAAACTCT GAAAACCCTT 
GATTTCAGTC AGGAAATGAG TGGGATTTTC AAAGATCATC CAATTTCAGG AGTCCCAGAG 
TACTCTGATC AAAGTGACTG CGAGGAACTT GATGCCCGAT TTCTTCCAGT CTCGCATCAT 
TTCTCTAGAA ACACTTCCAA TCCCACCAGT TTTCCCCATT TATCCTCTGA GGAACTGATG 
AATGACTGTG GGATTGAAGC TGAGACGTTA CCTGAGCTAG AAATTAGGTC AGATTCATCT 
CTTGGCACTT ACCAGAGTCC TGTCTCTAAA ACAAACCACA CTTTCAGATC AAACCAGGAA 
GAGATTAAAC ACAAGATCTC ACCTCAAACT TCACCCAGAA GCCCTGTAAT AAAAAACAGC 
CCTGAAAAAA TATACCAAGA TGCTACGAGT GGCGAAAAAT TAGATGGAAG GTTACACAGG 
CCTTACCACA ATCTGCAGCC TCCAACAGTC TCTCCCAGAA AAACAACCTC TGCTTCCTGC 
AACACTCAGG TTTCCATTCC AGCGCAGGCT ATTTCTAAAG CAAGCGTCTC CCCCTCAGAG 
CGATCGCCTC CTCCTACACC TCACAGAAAT TCAGCAATAA AGAATCACAG AAACTGCAAA 
ACCTCTCACA TGGATCCAGA TGACATCAGG AAAGGACAGC TGAATCATGC CTTGCCTGAT 
TTCTCCAAAG TAGAGCCGAA GGTGCGTTTC CCAAAAAGAA GTGAATACAA ACCTCCCAAA 
AGCCGAAGAC CCCCTCATGT TCGAACATCA TACCCGGGTC TTCCAGTGGT GTTCAAATCT 
CCTGCTGAGA TTGTGAAAGA GGTCCTGTTG AGCAGCTGTG ATGGGACTAC TGGGAGTCCA 
TCCTCCAGCG AGACTCAGAA GCACCTGCAC AATATCGTCC CAGAAGAGTT TCGCTGCCCA 
CAGCAGGCCA GCACACTGAT GCAGCAGCTA CAGGAGGACT ATAACAGGCT TCTTACCAAG 
TATGCAGAAG CTGAAAATAC TATTGATCGA CTTCGCCTTG AAGCAAAGGT GGGTCTCTCT 
TCGGAACCAC CCAAACCCAG TAGTACAGCT GTCCTGTCGG GAGTTATTCA GGAAGGGTCA 
AAGGTCATAA CCATCAGTTT TCCTCAAGCA CAGAGGGCAG AGTTCAGTAC AGGTTCTGTT 
CATCTGACCC AACAGAAGGA GCTCTCAGAA AACTCCAGAA AAGCAGCAAC TCGGCCTTCT 
TCTGTAAGCT CTATCAGCTC GAGGAGGTCT GGATCTCTTA CTCCAGACCA TCTGACAGAC 
ACTCTGTCCA AACAGAACCA AAGATTTCAG CTACAGGTGG ATGCATTTGA GTCGCTTTTA 
AAGGGTGGGA AGCTCAAGCC ATGTGAGCAG ACAAAGGGTC TCTCCACACT GGCTGAGGGG 
CAGGAGTCTC TTGAGAGGGC TTATCTAAAT GCACGAGTCC AATATCAGAT GCTGCAGCAA 
CGACAAGGCA GACATGCCAT CTTTGACCCA GACAGGGATC TAGAGGGTCA GATCTTCAGG 
TCAGGGATGC GATTAGAGGA GCTGAAAGAG TGGCTGGAAC AAGCAGAACA GAACCAACCT 
GTTTCTGAAC CTACTTTATC CCCATATCCT CCTTCAGATG TCCTTTCTGC ATCCATGCTG 
GAGCCTGAAC CAGTGCCTGA GATCCCCCTG TCAGCAGTCT GTCCTGAGGC TTGTGTTGGT 
GTGGAGGTCA GTTCAGTCAG TGGAGAGAGT GATAGTGGTA GAGAGGAAAT GGAAATGCTT 
CCGTTTGTTC TTCATCCCCT CGATAACAAA CACCAACGTG TGGAGAAAGA TTTCAGTGAA 
CTCATGGACT GCTATCAGAG TGTTAAAGAG CTCCCCGGGA CACTGGATCA GGCTGTGACT 
TTAAAGAGCC ATGACTTGCT GGATTTTGGA AGCTCTGTTC CTCCTGGGAA AAAAAGTGTA 
ACGGACGGAC AACCACTCAA GACTGTTAAT GAGAGAGAGA TGAGAAAACC AGCTCAGCAG 
CAGCCGGCAG CAGGTTGTGT CCCTCCCTCA TGCCCATCTG TCAGTTCACA GGTGTGCGAC 
TCTATGGAAA CTCACCCAGA GGCTTTTTGC CACTCCTATG TATTGGCAGG AGAATCGACC 
AGAGAGAGTA AAACAGCAGG AAACATGAAA GCTCAAAGCA CTAGCTTGAC CAGCCTGGAA 
GAGAGAACAC ACCACAGAAC TGTAGGCCTG AAAGCGAAGG CGAAGACTCT AAGAGCGCCC 
CCTATGGATG GGGTCATGTC TCCAGAAACA GACAGTGGCT TTATGGGCTC TGAAAGCAGT 
CAGCTCACTG CTGCATTACA CAGTCCTCTC CACCAGAGGG CAGTAATAAG TTCCATCAGA 
CTGTCTGACC CTCACACAAA GGACACAGAG AGACCAGAGT CTGCTTCTCC TTCTGGGCAG 
CCTTTCATTG TCTCTCCTTC TCCATCCCAA CCTTCTCCAA ACACCACAAA AAAGCACATT 
GATACATCAG GAAAATGCAC AGGTCCTTAT AAGAAAATAG GAGGGGAGGA AAGGTGTTCT 
TTTTCCAGTC TTTCTTCCTC CTTATCCCCC CTTCACTGGC CACGTAGCAG CATTCTGCCC 
TGGACAAGCA GCCTGACCAG TCAATCAGAG CGTGGAGCTG ATGAGGAAGA AGAAAAAGCA 
CAGAAAGACA AATACCCACA ACCGACCAAT CAGCAACTCC GCTCTCACAG AAGCCCCTCC 
CTCCACAGGC CTTATCACCA CAGTTCTGCT CAGCTAACAA ATCACCAAAA GGCTCTTCAA 
TCTCTGCAAA AAGAAGTGAA CCGATTGAAA GAGAGATTAG AGGGAAATCT GAGACTATCA 
AAACCTGCCA GACCTATGAG AACAGAAGTT TCTGCCTCTG AAGACACCAG AGGTCACACA 
CATCCACAGA CATCAACACC TCAGAGGCAT GATTTAAGGT CTTCAAAGAG GAGGAGAAGG 
CAGACAGAGA GAGCTGGCAA TGGAGAAAAA GAGAGAGGAA TTTCACCTAG ACCAGTCCTC 
AGACAAAGAT CAGCATCTTT ACCTCGACAT CGGCCTGAAA CTGATATGAC ATCTGATTCT 
GAGCATGCTC AATCTGAGCC AAGACCATCA ACGTCTAGAT ATGTCCCAGT GTCGCCTGTG 
AAATCAAGAG GAAGAAGACA AACCAGGAGA TTTGAATGTC ACAAAGAGGA TAGTACATCA 
GGCAGATCTG ACAACAGCGA TGAAGCAGAA CCAAACGGAG GAGCAGAATC TCTGTGTCCT 
GATTGCATAT CCGATCGTAC CAGTGCTTTG ACACGTCTCC TGAGGAGCAG GAGCTCTCCA 
CATCTTGCAC AGACTTCCTG TAGCCATTGC CCATTGTGTG GTGCATTACA AGCCATTCAA 
AGAGCCTCTA AGCCAGCTCC AGTCTCCAGT CCCAGAGCGA GTCAGCCAAT GAGATCCTTA 
CAGAGAGAAA AAGGAGGCGT GAACGTGACA GTGGCTCCTC CCCCTTTATT GCAGAGAGCT 
GTTCCAGTTG TCCCATATGT GCCTGTATAT CCTTCAACTC TATATTTCTC CAGCCCAATG 
ACTCCACCGG CATACCCACA ACCCCTCAAC ATCTCATCAA ATCCCGTCAG GCCTGAGAGG 
TCAGGGGTCA GAGGTCACCG CAGACGCTCT TCCTCCATGG AGTATCATCA GGATTCTATG 
AGCAGCGCTT TGACCCGTGC CATCACTGCA GCGAGACACA TGAGAGAAGC CTCACAGCGC 
ATGGCCAGCA GCCTCCACAC TGCATCCTGC AGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161173.1
CDS	411..4769
Translation	MERSRGGTSTGVLVWTPAPQLSSPSASEGSDFSWDEEPDQDFQSQMDENGIIGLAERQGQQEREEEEVAEELLWDVKTPEPEHGPPPAVEEISCHLSELLDSEPLSQATGDHYYSPSLQEEHQTDDDRSVLSEDWADDEGGTGSPVKHKLSSFPERDTALDMTDEETEEKNTQEDAQRFMDQSFHVTEERAAVFQTLKTLDFSQEMSGIFKDHPISGVPEYSDQSDCEELDARFLPVSHHFSRNTSNPTSFPHLSSEELMNDCGIEAETLPELEIRSDSSLGTYQSPVSKTNHTFRSNQEEIKHKISPQTSPRSPVIKNSPEKIYQDATSGEKLDGRLHRPYHNLQPPTVSPRKTTSASCNTQVSIPAQAISKASVSPSERSPPPTPHRNSAIKNHRNCKTSHMDPDDIRKGQLNHALPDFSKVEPKVRFPKRSEYKPPKSRRPPHVRTSYPGLPVVFKSPAEIVKEVLLSSCDGTTGSPSSSETQKHLHNIVPEEFRCPQQASTLMQQLQEDYNRLLTKYAEAENTIDRLRLEAKVGLSSEPPKPSSTAVLSGVIQEGSKVITISFPQAQRAEFSTGSVHLTQQKELSENSRKAATRPSSVSSISSRRSGSLTPDHLTDTLSKQNQRFQLQVDAFESLLKGGKLKPCEQTKGLSTLAEGQESLERAYLNARVQYQMLQQRQGRHAIFDPDRDLEGQIFRSGMRLEELKEWLEQAEQNQPVSEPTLSPYPPSDVLSASMLEPEPVPEIPLSAVCPEACVGVEVSSVSGESDSGREEMEMLPFVLHPLDNKHQRVEKDFSELMDCYQSVKELPGTLDQAVTLKSHDLLDFGSSVPPGKKSVTDGQPLKTVNEREMRKPAQQQPAAGCVPPSCPSVSSQVCDSMETHPEAFCHSYVLAGESTRESKTAGNMKAQSTSLTSLEERTHHRTVGLKAKAKTLRAPPMDGVMSPETDSGFMGSESSQLTAALHSPLHQRAVISSIRLSDPHTKDTERPESASPSGQPFIVSPSPSQPSPNTTKKHIDTSGKCTGPYKKIGGEERCSFSSLSSSLSPLHWPRSSILPWTSSLTSQSERGADEEEEKAQKDKYPQPTNQQLRSHRSPSLHRPYHHSSAQLTNHQKALQSLQKEVNRLKERLEGNLRLSKPARPMRTEVSASEDTRGHTHPQTSTPQRHDLRSSKRRRRQTERAGNGEKERGISPRPVLRQRSASLPRHRPETDMTSDSEHAQSEPRPSTSRYVPVSPVKSRGRRQTRRFECHKEDSTSGRSDNSDEAEPNGGAESLCPDCISDRTSALTRLLRSRSSPHLAQTSCSHCPLCGALQAIQRASKPAPVSSPRASQPMRSLQREKGGVNVTVAPPPLLQRAVPVVPYVPVYPSTLYFSSPMTPPAYPQPLNISSNPVRPERSGVRGHRRRSSSMEYHQDSMSSALTRAITAARHMREASQRMASSLHTASCSY

Target ORF information:

RefSeq Version	XM_005161116.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio AT-hook transcription factor (akna), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161116.4

ORF Insert Sequence:

ATGGAGCGGA GCAGAGGAGG CACCTCTACA GGGGTTCTGG TTTGGACACC TGCCCCACAG 
CTTTCCTCTC CCTCAGCCTC AGAGGGGTCA GACTTCAGCT GGGATGAAGA ACCGGACCAG 
GACTTCCAAA GCCAGATGGA TGAGAATGGG ATCATTGGTC TGGCAGAGAG GCAAGGGCAG 
CAGGAGAGAG AAGAGGAGGA GGTTGCAGAG GAGCTGTTGT GGGATGTGAA GACCCCTGAG 
CCTGAGCATG GGCCGCCGCC TGCAGTAGAG GAGATAAGCT GTCATCTGAG CGAACTGCTG 
GACTCTGAAC CGCTCTCTCA GGCCACTGGA GACCACTACT ACTCTCCATC ACTGCAGGAA 
GAACACCAAA CAGACGATGA TCGTAGTGTG TTGTCTGAGG ATTGGGCTGA TGACGAGGGT 
GGTACAGGGA GCCCAGTAAA GCACAAATTA AGCTCTTTCC CTGAGAGAGA CACTGCATTA 
GACATGACCG ATGAGGAGAC AGAAGAGAAA AACACACAAG AAGATGCTCA GAGATTCATG 
GATCAATCAT TTCATGTCAC AGAGGAGAGG GCAGCTGTGT TTCAAACTCT GAAAACCCTT 
GATTTCAGTC AGGAAATGAG TGGGATTTTC AAAGATCATC CAATTTCAGG AGTCCCAGAG 
TACTCTGATC AAAGTGACTG CGAGGAACTT GATGCCCGAT TTCTTCCAGT CTCGCATCAT 
TTCTCTAGAA ACACTTCCAA TCCCACCAGT TTTCCCCATT TATCCTCTGA GGAACTGATG 
AATGACTGTG GGATTGAAGC TGAGACGTTA CCTGAGCTAG AAATTAGGTC AGATTCATCT 
CTTGGCACTT ACCAGAGTCC TGTCTCTAAA ACAAACCACA CTTTCAGATC AAACCAGGAA 
GAGATTAAAC ACAAGATCTC ACCTCAAACT TCACCCAGAA GCCCTGTAAT AAAAAACAGC 
CCTGAAAAAA TATACCAAGA TGCTACGAGT GGCGAAAAAT TAGATGGAAG GTTACACAGG 
CCTTACCACA ATCTGCAGCC TCCAACAGTC TCTCCCAGAA AAACAACCTC TGCTTCCTGC 
AACACTCAGG TTTCCATTCC AGCGCAGGCT ATTTCTAAAG CAAGCGTCTC CCCCTCAGAG 
CGATCGCCTC CTCCTACACC TCACAGAAAT TCAGCAATAA AGAATCACAG AAACTGCAAA 
ACCTCTCACA TGGATCCAGA TGACATCAGG AAAGGACAGC TGAATCATGC CTTGCCTGAT 
TTCTCCAAAG TAGAGCCGAA GGTGCGTTTC CCAAAAAGAA GTGAATACAA ACCTCCCAAA 
AGCCGAAGAC CCCCTCATGT TCGAACATCA TACCCGGGTC TTCCAGTGGT GTTCAAATCT 
CCTGCTGAGA TTGTGAAAGA GGTCCTGTTG AGCAGCTGTG ATGGGACTAC TGGGAGTCCA 
TCCTCCAGCG AGACTCAGAA GCACCTGCAC AATATCGTCC CAGAAGAGTT TCGCTGCCCA 
CAGCAGGCCA GCACACTGAT GCAGCAGCTA CAGGAGGACT ATAACAGGCT TCTTACCAAG 
TATGCAGAAG CTGAAAATAC TATTGATCGA CTTCGCCTTG AAGCAAAGGT GGGTCTCTCT 
TCGGAACCAC CCAAACCCAG TAGTACAGCT GTCCTGTCGG GAGTTATTCA GGAAGGGTCA 
AAGGTCATAA CCATCAGTTT TCCTCAAGCA CAGAGGGCAG AGTTCAGTAC AGGTTCTGTT 
CATCTGACCC AACAGAAGGA GCTCTCAGAA AACTCCAGAA AAGCAGCAAC TCGGCCTTCT 
TCTGTAAGCT CTATCAGCTC GAGGAGGTCT GGATCTCTTA CTCCAGACCA TCTGACAGAC 
ACTCTGTCCA AACAGAACCA AAGATTTCAG CTACAGGTGG ATGCATTTGA GTCGCTTTTA 
AAGGGTGGGA AGCTCAAGCC ATGTGAGCAG ACAAAGGGTC TCTCCACACT GGCTGAGGGG 
CAGGAGTCTC TTGAGAGGGC TTATCTAAAT GCACGAGTCC AATATCAGAT GCTGCAGCAA 
CGACAAGGCA GACATGCCAT CTTTGACCCA GACAGGGATC TAGAGGGTCA GATCTTCAGG 
TCAGGGATGC GATTAGAGGA GCTGAAAGAG TGGCTGGAAC AAGCAGAACA GAACCAACCT 
GTTTCTGAAC CTACTTTATC CCCATATCCT CCTTCAGATG TCCTTTCTGC ATCCATGCTG 
GAGCCTGAAC CAGTGCCTGA GATCCCCCTG TCAGCAGTCT GTCCTGAGGC TTGTGTTGGT 
GTGGAGGTCA GTTCAGTCAG TGGAGAGAGT GATAGTGGTA GAGAGGAAAT GGAAATGCTT 
CCGTTTGTTC TTCATCCCCT CGATAACAAA CACCAACGTG TGGAGAAAGA TTTCAGTGAA 
CTCATGGACT GCTATCAGAG TGTTAAAGAG CTCCCCGGGA CACTGGATCA GGCTGTGACT 
TTAAAGAGCC ATGACTTGCT GGATTTTGGA AGCTCTGTTC CTCCTGGGAA AAAAAGTGTA 
ACGGACGGAC AACCACTCAA GACTGTTAAT GAGAGAGAGA TGAGAAAACC AGCTCAGCAG 
CAGCCGGCAG CAGGTTGTGT CCCTCCCTCA TGCCCATCTG TCAGTTCACA GGTGTGCGAC 
TCTATGGAAA CTCACCCAGA GGCTTTTTGC CACTCCTATG TATTGGCAGG AGAATCGACC 
AGAGAGAGTA AAACAGCAGG AAACATGAAA GCTCAAAGCA CTAGCTTGAC CAGCCTGGAA 
GAGAGAACAC ACCACAGAAC TGTAGGCCTG AAAGCGAAGG CGAAGACTCT AAGAGCGCCC 
CCTATGGATG GGGTCATGTC TCCAGAAACA GACAGTGGCT TTATGGGCTC TGAAAGCAGT 
CAGCTCACTG CTGCATTACA CAGTCCTCTC CACCAGAGGG CAGTAATAAG TTCCATCAGA 
CTGTCTGACC CTCACACAAA GGACACAGAG AGACCAGAGT CTGCTTCTCC TTCTGGGCAG 
CCTTTCATTG TCTCTCCTTC TCCATCCCAA CCTTCTCCAA ACACCACAAA AAAGCACATT 
GATACATCAG GAAAATGCAC AGGTCCTTAT AAGAAAATAG GAGGGGAGGA AAGGTGTTCT 
TTTTCCAGTC TTTCTTCCTC CTTATCCCCC CTTCACTGGC CACGTAGCAG CATTCTGCCC 
TGGACAAGCA GCCTGACCAG TCAATCAGAG CGTGGAGCTG ATGAGGAAGA AGAAAAAGCA 
CAGAAAGACA AATACCCACA ACCGACCAAT CAGCAACTCC GCTCTCACAG AAGCCCCTCC 
CTCCACAGGC CTTATCACCA CAGTTCTGCT CAGCTAACAA ATCACCAAAA GGCTCTTCAA 
TCTCTGCAAA AAGAAGTGAA CCGATTGAAA GAGAGATTAG AGGGAAATCT GAGACTATCA 
AAACCTGCCA GACCTATGAG AACAGAAGTT TCTGCCTCTG AAGACACCAG AGGTCACACA 
CATCCACAGA CATCAACACC TCAGAGGCAT GATTTAAGGT CTTCAAAGAG GAGGAGAAGG 
CAGACAGAGA GAGCTGGCAA TGGAGAAAAA GAGAGAGGAA TTTCACCTAG ACCAGTCCTC 
AGACAAAGAT CAGCATCTTT ACCTCGACAT CGGCCTGAAA CTGATATGAC ATCTGATTCT 
GAGCATGCTC AATCTGAGCC AAGACCATCA ACGTCTAGAT ATGTCCCAGT GTCGCCTGTG 
AAATCAAGAG GAAGAAGACA AACCAGGAGA TTTGAATGTC ACAAAGAGGA TAGTACATCA 
GGCAGATCTG ACAACAGCGA TGAAGCAGAA CCAAACGGAG GAGCAGAATC TCTGTGTCCT 
GATTGCATAT CCGATCGTAC CAGTGCTTTG ACACGTCTCC TGAGGAGCAG GAGCTCTCCA 
CATCTTGCAC AGACTTCCTG TAGCCATTGC CCATTGTGTG GTGCATTACA AGCCATTCAA 
AGAGCCTCTA AGCCAGCTCC AGTCTCCAGT CCCAGAGCGA GTCAGCCAAT GAGATCCTTA 
CAGAGAGAAA AAGGAGGCGT GAACGTGACA GTGGCTCCTC CCCCTTTATT GCAGAGAGCT 
GTTCCAGTTG TCCCATATGT GCCTGTATAT CCTTCAACTC TATATTTCTC CAGCCCAATG 
ACTCCACCGG CATACCCACA ACCCCTCAAC ATCTCATCAA ATCCCGTCAG GCCTGAGAGG 
TCAGGGGTCA GAGGTCACCG CAGACGCTCT TCCTCCATGG AGTATCATCA GGATTCTATG 
AGCAGCGCTT TGACCCGTGC CATCACTGCA GCGAGACACA TGAGAGAAGC CTCACAGCGC 
ATGGCCAGCA GCCTCCACAC TGCATCCTGC AGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

akna ( XM_678843.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_683935.4 Danio rerio AT-hook transcription factor (akna), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa25697
Clone ID Related Accession (Same CDS sequence)	XM_009295258.3 , XM_009295258.2 , XM_009295256.3 , XM_005161116.4 , XM_678843.6 , XM_005161116.3 , XM_009295257.2 , XM_678843.7 , XM_017353013.1 , XM_009295256.2 , XM_009295257.3
Accession Version	XM_678843.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	AT-hook-containing transcription factor-like isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_678843.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGGA GCAGAGGAGG CACCTCTACA GGGGTTCTGG TTTGGACACC TGCCCCACAG 
CTTTCCTCTC CCTCAGCCTC AGAGGGGTCA GACTTCAGCT GGGATGAAGA ACCGGACCAG 
GACTTCCAAA GCCAGATGGA TGAGAATGGG ATCATTGGTC TGGCAGAGAG GCAAGGGCAG 
CAGGAGAGAG AAGAGGAGGA GGTTGCAGAG GAGCTGTTGT GGGATGTGAA GACCCCTGAG 
CCTGAGCATG GGCCGCCGCC TGCAGTAGAG GAGATAAGCT GTCATCTGAG CGAACTGCTG 
GACTCTGAAC CGCTCTCTCA GGCCACTGGA GACCACTACT ACTCTCCATC ACTGCAGGAA 
GAACACCAAA CAGACGATGA TCGTAGTGTG TTGTCTGAGG ATTGGGCTGA TGACGAGGGT 
GGTACAGGGA GCCCAGTAAA GCACAAATTA AGCTCTTTCC CTGAGAGAGA CACTGCATTA 
GACATGACCG ATGAGGAGAC AGAAGAGAAA AACACACAAG AAGATGCTCA GAGATTCATG 
GATCAATCAT TTCATGTCAC AGAGGAGAGG GCAGCTGTGT TTCAAACTCT GAAAACCCTT 
GATTTCAGTC AGGAAATGAG TGGGATTTTC AAAGATCATC CAATTTCAGG AGTCCCAGAG 
TACTCTGATC AAAGTGACTG CGAGGAACTT GATGCCCGAT TTCTTCCAGT CTCGCATCAT 
TTCTCTAGAA ACACTTCCAA TCCCACCAGT TTTCCCCATT TATCCTCTGA GGAACTGATG 
AATGACTGTG GGATTGAAGC TGAGACGTTA CCTGAGCTAG AAATTAGGTC AGATTCATCT 
CTTGGCACTT ACCAGAGTCC TGTCTCTAAA ACAAACCACA CTTTCAGATC AAACCAGGAA 
GAGATTAAAC ACAAGATCTC ACCTCAAACT TCACCCAGAA GCCCTGTAAT AAAAAACAGC 
CCTGAAAAAA TATACCAAGA TGCTACGAGT GGCGAAAAAT TAGATGGAAG GTTACACAGG 
CCTTACCACA ATCTGCAGCC TCCAACAGTC TCTCCCAGAA AAACAACCTC TGCTTCCTGC 
AACACTCAGG TTTCCATTCC AGCGCAGGCT ATTTCTAAAG CAAGCGTCTC CCCCTCAGAG 
CGATCGCCTC CTCCTACACC TCACAGAAAT TCAGCAATAA AGAATCACAG AAACTGCAAA 
ACCTCTCACA TGGATCCAGA TGACATCAGG AAAGGACAGC TGAATCATGC CTTGCCTGAT 
TTCTCCAAAG TAGAGCCGAA GGTGCGTTTC CCAAAAAGAA GTGAATACAA ACCTCCCAAA 
AGCCGAAGAC CCCCTCATGT TCGAACATCA TACCCGGGTC TTCCAGTGGT GTTCAAATCT 
CCTGCTGAGA TTGTGAAAGA GGTCCTGTTG AGCAGCTGTG ATGGGACTAC TGGGAGTCCA 
TCCTCCAGCG AGACTCAGAA GCACCTGCAC AATATCGTCC CAGAAGAGTT TCGCTGCCCA 
CAGCAGGCCA GCACACTGAT GCAGCAGCTA CAGGAGGACT ATAACAGGCT TCTTACCAAG 
TATGCAGAAG CTGAAAATAC TATTGATCGA CTTCGCCTTG AAGCAAAGGT GGGTCTCTCT 
TCGGAACCAC CCAAACCCAG TAGTACAGCT GTCCTGTCGG GAGTTATTCA GGAAGGGTCA 
AAGGTCATAA CCATCAGTTT TCCTCAAGCA CAGAGGGCAG AGTTCAGTAC AGGTTCTGTT 
CATCTGACCC AACAGAAGGA GCTCTCAGAA AACTCCAGAA AAGCAGCAAC TCGGCCTTCT 
TCTGTAAGCT CTATCAGCTC GAGGAGGTCT GGATCTCTTA CTCCAGACCA TCTGACAGAC 
ACTCTGTCCA AACAGAACCA AAGATTTCAG CTACAGGTGG ATGCATTTGA GTCGCTTTTA 
AAGGGTGGGA AGCTCAAGCC ATGTGAGCAG ACAAAGGGTC TCTCCACACT GGCTGAGGGG 
CAGGAGTCTC TTGAGAGGGC TTATCTAAAT GCACGAGTCC AATATCAGAT GCTGCAGCAA 
CGACAAGGCA GACATGCCAT CTTTGACCCA GACAGGGATC TAGAGGGTCA GATCTTCAGG 
TCAGGGATGC GATTAGAGGA GCTGAAAGAG TGGCTGGAAC AAGCAGAACA GAACCAACCT 
GTTTCTGAAC CTACTTTATC CCCATATCCT CCTTCAGATG TCCTTTCTGC ATCCATGCTG 
GAGCCTGAAC CAGTGCCTGA GATCCCCCTG TCAGCAGTCT GTCCTGAGGC TTGTGTTGGT 
GTGGAGGTCA GTTCAGTCAG TGGAGAGAGT GATAGTGGTA GAGAGGAAAT GGAAATGCTT 
CCGTTTGTTC TTCATCCCCT CGATAACAAA CACCAACGTG TGGAGAAAGA TTTCAGTGAA 
CTCATGGACT GCTATCAGAG TGTTAAAGAG CTCCCCGGGA CACTGGATCA GGCTGTGACT 
TTAAAGAGCC ATGACTTGCT GGATTTTGGA AGCTCTGTTC CTCCTGGGAA AAAAAGTGTA 
ACGGACGGAC AACCACTCAA GACTGTTAAT GAGAGAGAGA TGAGAAAACC AGCTCAGCAG 
CAGCCGGCAG CAGGTTGTGT CCCTCCCTCA TGCCCATCTG TCAGTTCACA GGTGTGCGAC 
TCTATGGAAA CTCACCCAGA GGCTTTTTGC CACTCCTATG TATTGGCAGG AGAATCGACC 
AGAGAGAGTA AAACAGCAGG AAACATGAAA GCTCAAAGCA CTAGCTTGAC CAGCCTGGAA 
GAGAGAACAC ACCACAGAAC TGTAGGCCTG AAAGCGAAGG CGAAGACTCT AAGAGCGCCC 
CCTATGGATG GGGTCATGTC TCCAGAAACA GACAGTGGCT TTATGGGCTC TGAAAGCAGT 
CAGCTCACTG CTGCATTACA CAGTCCTCTC CACCAGAGGG CAGTAATAAG TTCCATCAGA 
CTGTCTGACC CTCACACAAA GGACACAGAG AGACCAGAGT CTGCTTCTCC TTCTGGGCAG 
CCTTTCATTG TCTCTCCTTC TCCATCCCAA CCTTCTCCAA ACACCACAAA AAAGCACATT 
GATACATCAG GAAAATGCAC AGGTCCTTAT AAGAAAATAG GAGGGGAGGA AAGGTGTTCT 
TTTTCCAGTC TTTCTTCCTC CTTATCCCCC CTTCACTGGC CACGTAGCAG CATTCTGCCC 
TGGACAAGCA GCCTGACCAG TCAATCAGAG CGTGGAGCTG ATGAGGAAGA AGAAAAAGCA 
CAGAAAGACA AATACCCACA ACCGACCAAT CAGCAACTCC GCTCTCACAG AAGCCCCTCC 
CTCCACAGGC CTTATCACCA CAGTTCTGCT CAGCTAACAA ATCACCAAAA GGCTCTTCAA 
TCTCTGCAAA AAGAAGTGAA CCGATTGAAA GAGAGATTAG AGGGAAATCT GAGACTATCA 
AAACCTGCCA GACCTATGAG AACAGAAGTT TCTGCCTCTG AAGACACCAG AGGTCACACA 
CATCCACAGA CATCAACACC TCAGAGGCAT GATTTAAGGT CTTCAAAGAG GAGGAGAAGG 
CAGACAGAGA GAGCTGGCAA TGGAGAAAAA GAGAGAGGAA TTTCACCTAG ACCAGTCCTC 
AGACAAAGAT CAGCATCTTT ACCTCGACAT CGGCCTGAAA CTGATATGAC ATCTGATTCT 
GAGCATGCTC AATCTGAGCC AAGACCATCA ACGTCTAGAT ATGTCCCAGT GTCGCCTGTG 
AAATCAAGAG GAAGAAGACA AACCAGGAGA TTTGAATGTC ACAAAGAGGA TAGTACATCA 
GGCAGATCTG ACAACAGCGA TGAAGCAGAA CCAAACGGAG GAGCAGAATC TCTGTGTCCT 
GATTGCATAT CCGATCGTAC CAGTGCTTTG ACACGTCTCC TGAGGAGCAG GAGCTCTCCA 
CATCTTGCAC AGACTTCCTG TAGCCATTGC CCATTGTGTG GTGCATTACA AGCCATTCAA 
AGAGCCTCTA AGCCAGCTCC AGTCTCCAGT CCCAGAGCGA GTCAGCCAAT GAGATCCTTA 
CAGAGAGAAA AAGGAGGCGT GAACGTGACA GTGGCTCCTC CCCCTTTATT GCAGAGAGCT 
GTTCCAGTTG TCCCATATGT GCCTGTATAT CCTTCAACTC TATATTTCTC CAGCCCAATG 
ACTCCACCGG CATACCCACA ACCCCTCAAC ATCTCATCAA ATCCCGTCAG GCCTGAGAGG 
TCAGGGGTCA GAGGTCACCG CAGACGCTCT TCCTCCATGG AGTATCATCA GGATTCTATG 
AGCAGCGCTT TGACCCGTGC CATCACTGCA GCGAGACACA TGAGAGAAGC CTCACAGCGC 
ATGGCCAGCA GCCTCCACAC TGCATCCTGC AGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_683935.4
CDS	616..4974
Translation	MERSRGGTSTGVLVWTPAPQLSSPSASEGSDFSWDEEPDQDFQSQMDENGIIGLAERQGQQEREEEEVAEELLWDVKTPEPEHGPPPAVEEISCHLSELLDSEPLSQATGDHYYSPSLQEEHQTDDDRSVLSEDWADDEGGTGSPVKHKLSSFPERDTALDMTDEETEEKNTQEDAQRFMDQSFHVTEERAAVFQTLKTLDFSQEMSGIFKDHPISGVPEYSDQSDCEELDARFLPVSHHFSRNTSNPTSFPHLSSEELMNDCGIEAETLPELEIRSDSSLGTYQSPVSKTNHTFRSNQEEIKHKISPQTSPRSPVIKNSPEKIYQDATSGEKLDGRLHRPYHNLQPPTVSPRKTTSASCNTQVSIPAQAISKASVSPSERSPPPTPHRNSAIKNHRNCKTSHMDPDDIRKGQLNHALPDFSKVEPKVRFPKRSEYKPPKSRRPPHVRTSYPGLPVVFKSPAEIVKEVLLSSCDGTTGSPSSSETQKHLHNIVPEEFRCPQQASTLMQQLQEDYNRLLTKYAEAENTIDRLRLEAKVGLSSEPPKPSSTAVLSGVIQEGSKVITISFPQAQRAEFSTGSVHLTQQKELSENSRKAATRPSSVSSISSRRSGSLTPDHLTDTLSKQNQRFQLQVDAFESLLKGGKLKPCEQTKGLSTLAEGQESLERAYLNARVQYQMLQQRQGRHAIFDPDRDLEGQIFRSGMRLEELKEWLEQAEQNQPVSEPTLSPYPPSDVLSASMLEPEPVPEIPLSAVCPEACVGVEVSSVSGESDSGREEMEMLPFVLHPLDNKHQRVEKDFSELMDCYQSVKELPGTLDQAVTLKSHDLLDFGSSVPPGKKSVTDGQPLKTVNEREMRKPAQQQPAAGCVPPSCPSVSSQVCDSMETHPEAFCHSYVLAGESTRESKTAGNMKAQSTSLTSLEERTHHRTVGLKAKAKTLRAPPMDGVMSPETDSGFMGSESSQLTAALHSPLHQRAVISSIRLSDPHTKDTERPESASPSGQPFIVSPSPSQPSPNTTKKHIDTSGKCTGPYKKIGGEERCSFSSLSSSLSPLHWPRSSILPWTSSLTSQSERGADEEEEKAQKDKYPQPTNQQLRSHRSPSLHRPYHHSSAQLTNHQKALQSLQKEVNRLKERLEGNLRLSKPARPMRTEVSASEDTRGHTHPQTSTPQRHDLRSSKRRRRQTERAGNGEKERGISPRPVLRQRSASLPRHRPETDMTSDSEHAQSEPRPSTSRYVPVSPVKSRGRRQTRRFECHKEDSTSGRSDNSDEAEPNGGAESLCPDCISDRTSALTRLLRSRSSPHLAQTSCSHCPLCGALQAIQRASKPAPVSSPRASQPMRSLQREKGGVNVTVAPPPLLQRAVPVVPYVPVYPSTLYFSSPMTPPAYPQPLNISSNPVRPERSGVRGHRRRSSSMEYHQDSMSSALTRAITAARHMREASQRMASSLHTASCSY

Target ORF information:

RefSeq Version	XM_678843.6
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio AT-hook transcription factor (akna), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_678843.6

ORF Insert Sequence:

ATGGAGCGGA GCAGAGGAGG CACCTCTACA GGGGTTCTGG TTTGGACACC TGCCCCACAG 
CTTTCCTCTC CCTCAGCCTC AGAGGGGTCA GACTTCAGCT GGGATGAAGA ACCGGACCAG 
GACTTCCAAA GCCAGATGGA TGAGAATGGG ATCATTGGTC TGGCAGAGAG GCAAGGGCAG 
CAGGAGAGAG AAGAGGAGGA GGTTGCAGAG GAGCTGTTGT GGGATGTGAA GACCCCTGAG 
CCTGAGCATG GGCCGCCGCC TGCAGTAGAG GAGATAAGCT GTCATCTGAG CGAACTGCTG 
GACTCTGAAC CGCTCTCTCA GGCCACTGGA GACCACTACT ACTCTCCATC ACTGCAGGAA 
GAACACCAAA CAGACGATGA TCGTAGTGTG TTGTCTGAGG ATTGGGCTGA TGACGAGGGT 
GGTACAGGGA GCCCAGTAAA GCACAAATTA AGCTCTTTCC CTGAGAGAGA CACTGCATTA 
GACATGACCG ATGAGGAGAC AGAAGAGAAA AACACACAAG AAGATGCTCA GAGATTCATG 
GATCAATCAT TTCATGTCAC AGAGGAGAGG GCAGCTGTGT TTCAAACTCT GAAAACCCTT 
GATTTCAGTC AGGAAATGAG TGGGATTTTC AAAGATCATC CAATTTCAGG AGTCCCAGAG 
TACTCTGATC AAAGTGACTG CGAGGAACTT GATGCCCGAT TTCTTCCAGT CTCGCATCAT 
TTCTCTAGAA ACACTTCCAA TCCCACCAGT TTTCCCCATT TATCCTCTGA GGAACTGATG 
AATGACTGTG GGATTGAAGC TGAGACGTTA CCTGAGCTAG AAATTAGGTC AGATTCATCT 
CTTGGCACTT ACCAGAGTCC TGTCTCTAAA ACAAACCACA CTTTCAGATC AAACCAGGAA 
GAGATTAAAC ACAAGATCTC ACCTCAAACT TCACCCAGAA GCCCTGTAAT AAAAAACAGC 
CCTGAAAAAA TATACCAAGA TGCTACGAGT GGCGAAAAAT TAGATGGAAG GTTACACAGG 
CCTTACCACA ATCTGCAGCC TCCAACAGTC TCTCCCAGAA AAACAACCTC TGCTTCCTGC 
AACACTCAGG TTTCCATTCC AGCGCAGGCT ATTTCTAAAG CAAGCGTCTC CCCCTCAGAG 
CGATCGCCTC CTCCTACACC TCACAGAAAT TCAGCAATAA AGAATCACAG AAACTGCAAA 
ACCTCTCACA TGGATCCAGA TGACATCAGG AAAGGACAGC TGAATCATGC CTTGCCTGAT 
TTCTCCAAAG TAGAGCCGAA GGTGCGTTTC CCAAAAAGAA GTGAATACAA ACCTCCCAAA 
AGCCGAAGAC CCCCTCATGT TCGAACATCA TACCCGGGTC TTCCAGTGGT GTTCAAATCT 
CCTGCTGAGA TTGTGAAAGA GGTCCTGTTG AGCAGCTGTG ATGGGACTAC TGGGAGTCCA 
TCCTCCAGCG AGACTCAGAA GCACCTGCAC AATATCGTCC CAGAAGAGTT TCGCTGCCCA 
CAGCAGGCCA GCACACTGAT GCAGCAGCTA CAGGAGGACT ATAACAGGCT TCTTACCAAG 
TATGCAGAAG CTGAAAATAC TATTGATCGA CTTCGCCTTG AAGCAAAGGT GGGTCTCTCT 
TCGGAACCAC CCAAACCCAG TAGTACAGCT GTCCTGTCGG GAGTTATTCA GGAAGGGTCA 
AAGGTCATAA CCATCAGTTT TCCTCAAGCA CAGAGGGCAG AGTTCAGTAC AGGTTCTGTT 
CATCTGACCC AACAGAAGGA GCTCTCAGAA AACTCCAGAA AAGCAGCAAC TCGGCCTTCT 
TCTGTAAGCT CTATCAGCTC GAGGAGGTCT GGATCTCTTA CTCCAGACCA TCTGACAGAC 
ACTCTGTCCA AACAGAACCA AAGATTTCAG CTACAGGTGG ATGCATTTGA GTCGCTTTTA 
AAGGGTGGGA AGCTCAAGCC ATGTGAGCAG ACAAAGGGTC TCTCCACACT GGCTGAGGGG 
CAGGAGTCTC TTGAGAGGGC TTATCTAAAT GCACGAGTCC AATATCAGAT GCTGCAGCAA 
CGACAAGGCA GACATGCCAT CTTTGACCCA GACAGGGATC TAGAGGGTCA GATCTTCAGG 
TCAGGGATGC GATTAGAGGA GCTGAAAGAG TGGCTGGAAC AAGCAGAACA GAACCAACCT 
GTTTCTGAAC CTACTTTATC CCCATATCCT CCTTCAGATG TCCTTTCTGC ATCCATGCTG 
GAGCCTGAAC CAGTGCCTGA GATCCCCCTG TCAGCAGTCT GTCCTGAGGC TTGTGTTGGT 
GTGGAGGTCA GTTCAGTCAG TGGAGAGAGT GATAGTGGTA GAGAGGAAAT GGAAATGCTT 
CCGTTTGTTC TTCATCCCCT CGATAACAAA CACCAACGTG TGGAGAAAGA TTTCAGTGAA 
CTCATGGACT GCTATCAGAG TGTTAAAGAG CTCCCCGGGA CACTGGATCA GGCTGTGACT 
TTAAAGAGCC ATGACTTGCT GGATTTTGGA AGCTCTGTTC CTCCTGGGAA AAAAAGTGTA 
ACGGACGGAC AACCACTCAA GACTGTTAAT GAGAGAGAGA TGAGAAAACC AGCTCAGCAG 
CAGCCGGCAG CAGGTTGTGT CCCTCCCTCA TGCCCATCTG TCAGTTCACA GGTGTGCGAC 
TCTATGGAAA CTCACCCAGA GGCTTTTTGC CACTCCTATG TATTGGCAGG AGAATCGACC 
AGAGAGAGTA AAACAGCAGG AAACATGAAA GCTCAAAGCA CTAGCTTGAC CAGCCTGGAA 
GAGAGAACAC ACCACAGAAC TGTAGGCCTG AAAGCGAAGG CGAAGACTCT AAGAGCGCCC 
CCTATGGATG GGGTCATGTC TCCAGAAACA GACAGTGGCT TTATGGGCTC TGAAAGCAGT 
CAGCTCACTG CTGCATTACA CAGTCCTCTC CACCAGAGGG CAGTAATAAG TTCCATCAGA 
CTGTCTGACC CTCACACAAA GGACACAGAG AGACCAGAGT CTGCTTCTCC TTCTGGGCAG 
CCTTTCATTG TCTCTCCTTC TCCATCCCAA CCTTCTCCAA ACACCACAAA AAAGCACATT 
GATACATCAG GAAAATGCAC AGGTCCTTAT AAGAAAATAG GAGGGGAGGA AAGGTGTTCT 
TTTTCCAGTC TTTCTTCCTC CTTATCCCCC CTTCACTGGC CACGTAGCAG CATTCTGCCC 
TGGACAAGCA GCCTGACCAG TCAATCAGAG CGTGGAGCTG ATGAGGAAGA AGAAAAAGCA 
CAGAAAGACA AATACCCACA ACCGACCAAT CAGCAACTCC GCTCTCACAG AAGCCCCTCC 
CTCCACAGGC CTTATCACCA CAGTTCTGCT CAGCTAACAA ATCACCAAAA GGCTCTTCAA 
TCTCTGCAAA AAGAAGTGAA CCGATTGAAA GAGAGATTAG AGGGAAATCT GAGACTATCA 
AAACCTGCCA GACCTATGAG AACAGAAGTT TCTGCCTCTG AAGACACCAG AGGTCACACA 
CATCCACAGA CATCAACACC TCAGAGGCAT GATTTAAGGT CTTCAAAGAG GAGGAGAAGG 
CAGACAGAGA GAGCTGGCAA TGGAGAAAAA GAGAGAGGAA TTTCACCTAG ACCAGTCCTC 
AGACAAAGAT CAGCATCTTT ACCTCGACAT CGGCCTGAAA CTGATATGAC ATCTGATTCT 
GAGCATGCTC AATCTGAGCC AAGACCATCA ACGTCTAGAT ATGTCCCAGT GTCGCCTGTG 
AAATCAAGAG GAAGAAGACA AACCAGGAGA TTTGAATGTC ACAAAGAGGA TAGTACATCA 
GGCAGATCTG ACAACAGCGA TGAAGCAGAA CCAAACGGAG GAGCAGAATC TCTGTGTCCT 
GATTGCATAT CCGATCGTAC CAGTGCTTTG ACACGTCTCC TGAGGAGCAG GAGCTCTCCA 
CATCTTGCAC AGACTTCCTG TAGCCATTGC CCATTGTGTG GTGCATTACA AGCCATTCAA 
AGAGCCTCTA AGCCAGCTCC AGTCTCCAGT CCCAGAGCGA GTCAGCCAAT GAGATCCTTA 
CAGAGAGAAA AAGGAGGCGT GAACGTGACA GTGGCTCCTC CCCCTTTATT GCAGAGAGCT 
GTTCCAGTTG TCCCATATGT GCCTGTATAT CCTTCAACTC TATATTTCTC CAGCCCAATG 
ACTCCACCGG CATACCCACA ACCCCTCAAC ATCTCATCAA ATCCCGTCAG GCCTGAGAGG 
TCAGGGGTCA GAGGTCACCG CAGACGCTCT TCCTCCATGG AGTATCATCA GGATTCTATG 
AGCAGCGCTT TGACCCGTGC CATCACTGCA GCGAGACACA TGAGAGAAGC CTCACAGCGC 
ATGGCCAGCA GCCTCCACAC TGCATCCTGC AGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

akna ( XM_005161116.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161173.1 Danio rerio AT-hook transcription factor (akna), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa25697
Clone ID Related Accession (Same CDS sequence)	XM_009295258.3 , XM_009295258.2 , XM_009295256.3 , XM_005161116.4 , XM_678843.6 , XM_005161116.3 , XM_009295257.2 , XM_678843.7 , XM_017353013.1 , XM_009295256.2 , XM_009295257.3
Accession Version	XM_005161116.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	AT-hook-containing transcription factor-like isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005161116.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGGA GCAGAGGAGG CACCTCTACA GGGGTTCTGG TTTGGACACC TGCCCCACAG 
CTTTCCTCTC CCTCAGCCTC AGAGGGGTCA GACTTCAGCT GGGATGAAGA ACCGGACCAG 
GACTTCCAAA GCCAGATGGA TGAGAATGGG ATCATTGGTC TGGCAGAGAG GCAAGGGCAG 
CAGGAGAGAG AAGAGGAGGA GGTTGCAGAG GAGCTGTTGT GGGATGTGAA GACCCCTGAG 
CCTGAGCATG GGCCGCCGCC TGCAGTAGAG GAGATAAGCT GTCATCTGAG CGAACTGCTG 
GACTCTGAAC CGCTCTCTCA GGCCACTGGA GACCACTACT ACTCTCCATC ACTGCAGGAA 
GAACACCAAA CAGACGATGA TCGTAGTGTG TTGTCTGAGG ATTGGGCTGA TGACGAGGGT 
GGTACAGGGA GCCCAGTAAA GCACAAATTA AGCTCTTTCC CTGAGAGAGA CACTGCATTA 
GACATGACCG ATGAGGAGAC AGAAGAGAAA AACACACAAG AAGATGCTCA GAGATTCATG 
GATCAATCAT TTCATGTCAC AGAGGAGAGG GCAGCTGTGT TTCAAACTCT GAAAACCCTT 
GATTTCAGTC AGGAAATGAG TGGGATTTTC AAAGATCATC CAATTTCAGG AGTCCCAGAG 
TACTCTGATC AAAGTGACTG CGAGGAACTT GATGCCCGAT TTCTTCCAGT CTCGCATCAT 
TTCTCTAGAA ACACTTCCAA TCCCACCAGT TTTCCCCATT TATCCTCTGA GGAACTGATG 
AATGACTGTG GGATTGAAGC TGAGACGTTA CCTGAGCTAG AAATTAGGTC AGATTCATCT 
CTTGGCACTT ACCAGAGTCC TGTCTCTAAA ACAAACCACA CTTTCAGATC AAACCAGGAA 
GAGATTAAAC ACAAGATCTC ACCTCAAACT TCACCCAGAA GCCCTGTAAT AAAAAACAGC 
CCTGAAAAAA TATACCAAGA TGCTACGAGT GGCGAAAAAT TAGATGGAAG GTTACACAGG 
CCTTACCACA ATCTGCAGCC TCCAACAGTC TCTCCCAGAA AAACAACCTC TGCTTCCTGC 
AACACTCAGG TTTCCATTCC AGCGCAGGCT ATTTCTAAAG CAAGCGTCTC CCCCTCAGAG 
CGATCGCCTC CTCCTACACC TCACAGAAAT TCAGCAATAA AGAATCACAG AAACTGCAAA 
ACCTCTCACA TGGATCCAGA TGACATCAGG AAAGGACAGC TGAATCATGC CTTGCCTGAT 
TTCTCCAAAG TAGAGCCGAA GGTGCGTTTC CCAAAAAGAA GTGAATACAA ACCTCCCAAA 
AGCCGAAGAC CCCCTCATGT TCGAACATCA TACCCGGGTC TTCCAGTGGT GTTCAAATCT 
CCTGCTGAGA TTGTGAAAGA GGTCCTGTTG AGCAGCTGTG ATGGGACTAC TGGGAGTCCA 
TCCTCCAGCG AGACTCAGAA GCACCTGCAC AATATCGTCC CAGAAGAGTT TCGCTGCCCA 
CAGCAGGCCA GCACACTGAT GCAGCAGCTA CAGGAGGACT ATAACAGGCT TCTTACCAAG 
TATGCAGAAG CTGAAAATAC TATTGATCGA CTTCGCCTTG AAGCAAAGGT GGGTCTCTCT 
TCGGAACCAC CCAAACCCAG TAGTACAGCT GTCCTGTCGG GAGTTATTCA GGAAGGGTCA 
AAGGTCATAA CCATCAGTTT TCCTCAAGCA CAGAGGGCAG AGTTCAGTAC AGGTTCTGTT 
CATCTGACCC AACAGAAGGA GCTCTCAGAA AACTCCAGAA AAGCAGCAAC TCGGCCTTCT 
TCTGTAAGCT CTATCAGCTC GAGGAGGTCT GGATCTCTTA CTCCAGACCA TCTGACAGAC 
ACTCTGTCCA AACAGAACCA AAGATTTCAG CTACAGGTGG ATGCATTTGA GTCGCTTTTA 
AAGGGTGGGA AGCTCAAGCC ATGTGAGCAG ACAAAGGGTC TCTCCACACT GGCTGAGGGG 
CAGGAGTCTC TTGAGAGGGC TTATCTAAAT GCACGAGTCC AATATCAGAT GCTGCAGCAA 
CGACAAGGCA GACATGCCAT CTTTGACCCA GACAGGGATC TAGAGGGTCA GATCTTCAGG 
TCAGGGATGC GATTAGAGGA GCTGAAAGAG TGGCTGGAAC AAGCAGAACA GAACCAACCT 
GTTTCTGAAC CTACTTTATC CCCATATCCT CCTTCAGATG TCCTTTCTGC ATCCATGCTG 
GAGCCTGAAC CAGTGCCTGA GATCCCCCTG TCAGCAGTCT GTCCTGAGGC TTGTGTTGGT 
GTGGAGGTCA GTTCAGTCAG TGGAGAGAGT GATAGTGGTA GAGAGGAAAT GGAAATGCTT 
CCGTTTGTTC TTCATCCCCT CGATAACAAA CACCAACGTG TGGAGAAAGA TTTCAGTGAA 
CTCATGGACT GCTATCAGAG TGTTAAAGAG CTCCCCGGGA CACTGGATCA GGCTGTGACT 
TTAAAGAGCC ATGACTTGCT GGATTTTGGA AGCTCTGTTC CTCCTGGGAA AAAAAGTGTA 
ACGGACGGAC AACCACTCAA GACTGTTAAT GAGAGAGAGA TGAGAAAACC AGCTCAGCAG 
CAGCCGGCAG CAGGTTGTGT CCCTCCCTCA TGCCCATCTG TCAGTTCACA GGTGTGCGAC 
TCTATGGAAA CTCACCCAGA GGCTTTTTGC CACTCCTATG TATTGGCAGG AGAATCGACC 
AGAGAGAGTA AAACAGCAGG AAACATGAAA GCTCAAAGCA CTAGCTTGAC CAGCCTGGAA 
GAGAGAACAC ACCACAGAAC TGTAGGCCTG AAAGCGAAGG CGAAGACTCT AAGAGCGCCC 
CCTATGGATG GGGTCATGTC TCCAGAAACA GACAGTGGCT TTATGGGCTC TGAAAGCAGT 
CAGCTCACTG CTGCATTACA CAGTCCTCTC CACCAGAGGG CAGTAATAAG TTCCATCAGA 
CTGTCTGACC CTCACACAAA GGACACAGAG AGACCAGAGT CTGCTTCTCC TTCTGGGCAG 
CCTTTCATTG TCTCTCCTTC TCCATCCCAA CCTTCTCCAA ACACCACAAA AAAGCACATT 
GATACATCAG GAAAATGCAC AGGTCCTTAT AAGAAAATAG GAGGGGAGGA AAGGTGTTCT 
TTTTCCAGTC TTTCTTCCTC CTTATCCCCC CTTCACTGGC CACGTAGCAG CATTCTGCCC 
TGGACAAGCA GCCTGACCAG TCAATCAGAG CGTGGAGCTG ATGAGGAAGA AGAAAAAGCA 
CAGAAAGACA AATACCCACA ACCGACCAAT CAGCAACTCC GCTCTCACAG AAGCCCCTCC 
CTCCACAGGC CTTATCACCA CAGTTCTGCT CAGCTAACAA ATCACCAAAA GGCTCTTCAA 
TCTCTGCAAA AAGAAGTGAA CCGATTGAAA GAGAGATTAG AGGGAAATCT GAGACTATCA 
AAACCTGCCA GACCTATGAG AACAGAAGTT TCTGCCTCTG AAGACACCAG AGGTCACACA 
CATCCACAGA CATCAACACC TCAGAGGCAT GATTTAAGGT CTTCAAAGAG GAGGAGAAGG 
CAGACAGAGA GAGCTGGCAA TGGAGAAAAA GAGAGAGGAA TTTCACCTAG ACCAGTCCTC 
AGACAAAGAT CAGCATCTTT ACCTCGACAT CGGCCTGAAA CTGATATGAC ATCTGATTCT 
GAGCATGCTC AATCTGAGCC AAGACCATCA ACGTCTAGAT ATGTCCCAGT GTCGCCTGTG 
AAATCAAGAG GAAGAAGACA AACCAGGAGA TTTGAATGTC ACAAAGAGGA TAGTACATCA 
GGCAGATCTG ACAACAGCGA TGAAGCAGAA CCAAACGGAG GAGCAGAATC TCTGTGTCCT 
GATTGCATAT CCGATCGTAC CAGTGCTTTG ACACGTCTCC TGAGGAGCAG GAGCTCTCCA 
CATCTTGCAC AGACTTCCTG TAGCCATTGC CCATTGTGTG GTGCATTACA AGCCATTCAA 
AGAGCCTCTA AGCCAGCTCC AGTCTCCAGT CCCAGAGCGA GTCAGCCAAT GAGATCCTTA 
CAGAGAGAAA AAGGAGGCGT GAACGTGACA GTGGCTCCTC CCCCTTTATT GCAGAGAGCT 
GTTCCAGTTG TCCCATATGT GCCTGTATAT CCTTCAACTC TATATTTCTC CAGCCCAATG 
ACTCCACCGG CATACCCACA ACCCCTCAAC ATCTCATCAA ATCCCGTCAG GCCTGAGAGG 
TCAGGGGTCA GAGGTCACCG CAGACGCTCT TCCTCCATGG AGTATCATCA GGATTCTATG 
AGCAGCGCTT TGACCCGTGC CATCACTGCA GCGAGACACA TGAGAGAAGC CTCACAGCGC 
ATGGCCAGCA GCCTCCACAC TGCATCCTGC AGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161173.1
CDS	413..4771
Translation	MERSRGGTSTGVLVWTPAPQLSSPSASEGSDFSWDEEPDQDFQSQMDENGIIGLAERQGQQEREEEEVAEELLWDVKTPEPEHGPPPAVEEISCHLSELLDSEPLSQATGDHYYSPSLQEEHQTDDDRSVLSEDWADDEGGTGSPVKHKLSSFPERDTALDMTDEETEEKNTQEDAQRFMDQSFHVTEERAAVFQTLKTLDFSQEMSGIFKDHPISGVPEYSDQSDCEELDARFLPVSHHFSRNTSNPTSFPHLSSEELMNDCGIEAETLPELEIRSDSSLGTYQSPVSKTNHTFRSNQEEIKHKISPQTSPRSPVIKNSPEKIYQDATSGEKLDGRLHRPYHNLQPPTVSPRKTTSASCNTQVSIPAQAISKASVSPSERSPPPTPHRNSAIKNHRNCKTSHMDPDDIRKGQLNHALPDFSKVEPKVRFPKRSEYKPPKSRRPPHVRTSYPGLPVVFKSPAEIVKEVLLSSCDGTTGSPSSSETQKHLHNIVPEEFRCPQQASTLMQQLQEDYNRLLTKYAEAENTIDRLRLEAKVGLSSEPPKPSSTAVLSGVIQEGSKVITISFPQAQRAEFSTGSVHLTQQKELSENSRKAATRPSSVSSISSRRSGSLTPDHLTDTLSKQNQRFQLQVDAFESLLKGGKLKPCEQTKGLSTLAEGQESLERAYLNARVQYQMLQQRQGRHAIFDPDRDLEGQIFRSGMRLEELKEWLEQAEQNQPVSEPTLSPYPPSDVLSASMLEPEPVPEIPLSAVCPEACVGVEVSSVSGESDSGREEMEMLPFVLHPLDNKHQRVEKDFSELMDCYQSVKELPGTLDQAVTLKSHDLLDFGSSVPPGKKSVTDGQPLKTVNEREMRKPAQQQPAAGCVPPSCPSVSSQVCDSMETHPEAFCHSYVLAGESTRESKTAGNMKAQSTSLTSLEERTHHRTVGLKAKAKTLRAPPMDGVMSPETDSGFMGSESSQLTAALHSPLHQRAVISSIRLSDPHTKDTERPESASPSGQPFIVSPSPSQPSPNTTKKHIDTSGKCTGPYKKIGGEERCSFSSLSSSLSPLHWPRSSILPWTSSLTSQSERGADEEEEKAQKDKYPQPTNQQLRSHRSPSLHRPYHHSSAQLTNHQKALQSLQKEVNRLKERLEGNLRLSKPARPMRTEVSASEDTRGHTHPQTSTPQRHDLRSSKRRRRQTERAGNGEKERGISPRPVLRQRSASLPRHRPETDMTSDSEHAQSEPRPSTSRYVPVSPVKSRGRRQTRRFECHKEDSTSGRSDNSDEAEPNGGAESLCPDCISDRTSALTRLLRSRSSPHLAQTSCSHCPLCGALQAIQRASKPAPVSSPRASQPMRSLQREKGGVNVTVAPPPLLQRAVPVVPYVPVYPSTLYFSSPMTPPAYPQPLNISSNPVRPERSGVRGHRRRSSSMEYHQDSMSSALTRAITAARHMREASQRMASSLHTASCSY

Target ORF information:

RefSeq Version	XM_005161116.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio AT-hook transcription factor (akna), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161116.3

ORF Insert Sequence:

ATGGAGCGGA GCAGAGGAGG CACCTCTACA GGGGTTCTGG TTTGGACACC TGCCCCACAG 
CTTTCCTCTC CCTCAGCCTC AGAGGGGTCA GACTTCAGCT GGGATGAAGA ACCGGACCAG 
GACTTCCAAA GCCAGATGGA TGAGAATGGG ATCATTGGTC TGGCAGAGAG GCAAGGGCAG 
CAGGAGAGAG AAGAGGAGGA GGTTGCAGAG GAGCTGTTGT GGGATGTGAA GACCCCTGAG 
CCTGAGCATG GGCCGCCGCC TGCAGTAGAG GAGATAAGCT GTCATCTGAG CGAACTGCTG 
GACTCTGAAC CGCTCTCTCA GGCCACTGGA GACCACTACT ACTCTCCATC ACTGCAGGAA 
GAACACCAAA CAGACGATGA TCGTAGTGTG TTGTCTGAGG ATTGGGCTGA TGACGAGGGT 
GGTACAGGGA GCCCAGTAAA GCACAAATTA AGCTCTTTCC CTGAGAGAGA CACTGCATTA 
GACATGACCG ATGAGGAGAC AGAAGAGAAA AACACACAAG AAGATGCTCA GAGATTCATG 
GATCAATCAT TTCATGTCAC AGAGGAGAGG GCAGCTGTGT TTCAAACTCT GAAAACCCTT 
GATTTCAGTC AGGAAATGAG TGGGATTTTC AAAGATCATC CAATTTCAGG AGTCCCAGAG 
TACTCTGATC AAAGTGACTG CGAGGAACTT GATGCCCGAT TTCTTCCAGT CTCGCATCAT 
TTCTCTAGAA ACACTTCCAA TCCCACCAGT TTTCCCCATT TATCCTCTGA GGAACTGATG 
AATGACTGTG GGATTGAAGC TGAGACGTTA CCTGAGCTAG AAATTAGGTC AGATTCATCT 
CTTGGCACTT ACCAGAGTCC TGTCTCTAAA ACAAACCACA CTTTCAGATC AAACCAGGAA 
GAGATTAAAC ACAAGATCTC ACCTCAAACT TCACCCAGAA GCCCTGTAAT AAAAAACAGC 
CCTGAAAAAA TATACCAAGA TGCTACGAGT GGCGAAAAAT TAGATGGAAG GTTACACAGG 
CCTTACCACA ATCTGCAGCC TCCAACAGTC TCTCCCAGAA AAACAACCTC TGCTTCCTGC 
AACACTCAGG TTTCCATTCC AGCGCAGGCT ATTTCTAAAG CAAGCGTCTC CCCCTCAGAG 
CGATCGCCTC CTCCTACACC TCACAGAAAT TCAGCAATAA AGAATCACAG AAACTGCAAA 
ACCTCTCACA TGGATCCAGA TGACATCAGG AAAGGACAGC TGAATCATGC CTTGCCTGAT 
TTCTCCAAAG TAGAGCCGAA GGTGCGTTTC CCAAAAAGAA GTGAATACAA ACCTCCCAAA 
AGCCGAAGAC CCCCTCATGT TCGAACATCA TACCCGGGTC TTCCAGTGGT GTTCAAATCT 
CCTGCTGAGA TTGTGAAAGA GGTCCTGTTG AGCAGCTGTG ATGGGACTAC TGGGAGTCCA 
TCCTCCAGCG AGACTCAGAA GCACCTGCAC AATATCGTCC CAGAAGAGTT TCGCTGCCCA 
CAGCAGGCCA GCACACTGAT GCAGCAGCTA CAGGAGGACT ATAACAGGCT TCTTACCAAG 
TATGCAGAAG CTGAAAATAC TATTGATCGA CTTCGCCTTG AAGCAAAGGT GGGTCTCTCT 
TCGGAACCAC CCAAACCCAG TAGTACAGCT GTCCTGTCGG GAGTTATTCA GGAAGGGTCA 
AAGGTCATAA CCATCAGTTT TCCTCAAGCA CAGAGGGCAG AGTTCAGTAC AGGTTCTGTT 
CATCTGACCC AACAGAAGGA GCTCTCAGAA AACTCCAGAA AAGCAGCAAC TCGGCCTTCT 
TCTGTAAGCT CTATCAGCTC GAGGAGGTCT GGATCTCTTA CTCCAGACCA TCTGACAGAC 
ACTCTGTCCA AACAGAACCA AAGATTTCAG CTACAGGTGG ATGCATTTGA GTCGCTTTTA 
AAGGGTGGGA AGCTCAAGCC ATGTGAGCAG ACAAAGGGTC TCTCCACACT GGCTGAGGGG 
CAGGAGTCTC TTGAGAGGGC TTATCTAAAT GCACGAGTCC AATATCAGAT GCTGCAGCAA 
CGACAAGGCA GACATGCCAT CTTTGACCCA GACAGGGATC TAGAGGGTCA GATCTTCAGG 
TCAGGGATGC GATTAGAGGA GCTGAAAGAG TGGCTGGAAC AAGCAGAACA GAACCAACCT 
GTTTCTGAAC CTACTTTATC CCCATATCCT CCTTCAGATG TCCTTTCTGC ATCCATGCTG 
GAGCCTGAAC CAGTGCCTGA GATCCCCCTG TCAGCAGTCT GTCCTGAGGC TTGTGTTGGT 
GTGGAGGTCA GTTCAGTCAG TGGAGAGAGT GATAGTGGTA GAGAGGAAAT GGAAATGCTT 
CCGTTTGTTC TTCATCCCCT CGATAACAAA CACCAACGTG TGGAGAAAGA TTTCAGTGAA 
CTCATGGACT GCTATCAGAG TGTTAAAGAG CTCCCCGGGA CACTGGATCA GGCTGTGACT 
TTAAAGAGCC ATGACTTGCT GGATTTTGGA AGCTCTGTTC CTCCTGGGAA AAAAAGTGTA 
ACGGACGGAC AACCACTCAA GACTGTTAAT GAGAGAGAGA TGAGAAAACC AGCTCAGCAG 
CAGCCGGCAG CAGGTTGTGT CCCTCCCTCA TGCCCATCTG TCAGTTCACA GGTGTGCGAC 
TCTATGGAAA CTCACCCAGA GGCTTTTTGC CACTCCTATG TATTGGCAGG AGAATCGACC 
AGAGAGAGTA AAACAGCAGG AAACATGAAA GCTCAAAGCA CTAGCTTGAC CAGCCTGGAA 
GAGAGAACAC ACCACAGAAC TGTAGGCCTG AAAGCGAAGG CGAAGACTCT AAGAGCGCCC 
CCTATGGATG GGGTCATGTC TCCAGAAACA GACAGTGGCT TTATGGGCTC TGAAAGCAGT 
CAGCTCACTG CTGCATTACA CAGTCCTCTC CACCAGAGGG CAGTAATAAG TTCCATCAGA 
CTGTCTGACC CTCACACAAA GGACACAGAG AGACCAGAGT CTGCTTCTCC TTCTGGGCAG 
CCTTTCATTG TCTCTCCTTC TCCATCCCAA CCTTCTCCAA ACACCACAAA AAAGCACATT 
GATACATCAG GAAAATGCAC AGGTCCTTAT AAGAAAATAG GAGGGGAGGA AAGGTGTTCT 
TTTTCCAGTC TTTCTTCCTC CTTATCCCCC CTTCACTGGC CACGTAGCAG CATTCTGCCC 
TGGACAAGCA GCCTGACCAG TCAATCAGAG CGTGGAGCTG ATGAGGAAGA AGAAAAAGCA 
CAGAAAGACA AATACCCACA ACCGACCAAT CAGCAACTCC GCTCTCACAG AAGCCCCTCC 
CTCCACAGGC CTTATCACCA CAGTTCTGCT CAGCTAACAA ATCACCAAAA GGCTCTTCAA 
TCTCTGCAAA AAGAAGTGAA CCGATTGAAA GAGAGATTAG AGGGAAATCT GAGACTATCA 
AAACCTGCCA GACCTATGAG AACAGAAGTT TCTGCCTCTG AAGACACCAG AGGTCACACA 
CATCCACAGA CATCAACACC TCAGAGGCAT GATTTAAGGT CTTCAAAGAG GAGGAGAAGG 
CAGACAGAGA GAGCTGGCAA TGGAGAAAAA GAGAGAGGAA TTTCACCTAG ACCAGTCCTC 
AGACAAAGAT CAGCATCTTT ACCTCGACAT CGGCCTGAAA CTGATATGAC ATCTGATTCT 
GAGCATGCTC AATCTGAGCC AAGACCATCA ACGTCTAGAT ATGTCCCAGT GTCGCCTGTG 
AAATCAAGAG GAAGAAGACA AACCAGGAGA TTTGAATGTC ACAAAGAGGA TAGTACATCA 
GGCAGATCTG ACAACAGCGA TGAAGCAGAA CCAAACGGAG GAGCAGAATC TCTGTGTCCT 
GATTGCATAT CCGATCGTAC CAGTGCTTTG ACACGTCTCC TGAGGAGCAG GAGCTCTCCA 
CATCTTGCAC AGACTTCCTG TAGCCATTGC CCATTGTGTG GTGCATTACA AGCCATTCAA 
AGAGCCTCTA AGCCAGCTCC AGTCTCCAGT CCCAGAGCGA GTCAGCCAAT GAGATCCTTA 
CAGAGAGAAA AAGGAGGCGT GAACGTGACA GTGGCTCCTC CCCCTTTATT GCAGAGAGCT 
GTTCCAGTTG TCCCATATGT GCCTGTATAT CCTTCAACTC TATATTTCTC CAGCCCAATG 
ACTCCACCGG CATACCCACA ACCCCTCAAC ATCTCATCAA ATCCCGTCAG GCCTGAGAGG 
TCAGGGGTCA GAGGTCACCG CAGACGCTCT TCCTCCATGG AGTATCATCA GGATTCTATG 
AGCAGCGCTT TGACCCGTGC CATCACTGCA GCGAGACACA TGAGAGAAGC CTCACAGCGC 
ATGGCCAGCA GCCTCCACAC TGCATCCTGC AGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

akna ( XM_009295257.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293532.1 Danio rerio AT-hook transcription factor (akna), transcript variant X5, mRNA.

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CloneID	ODa25697
Clone ID Related Accession (Same CDS sequence)	XM_009295258.3 , XM_009295258.2 , XM_009295256.3 , XM_005161116.4 , XM_678843.6 , XM_005161116.3 , XM_009295257.2 , XM_678843.7 , XM_017353013.1 , XM_009295256.2 , XM_009295257.3
Accession Version	XM_009295257.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	AT-hook-containing transcription factor-like isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295257.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGGA GCAGAGGAGG CACCTCTACA GGGGTTCTGG TTTGGACACC TGCCCCACAG 
CTTTCCTCTC CCTCAGCCTC AGAGGGGTCA GACTTCAGCT GGGATGAAGA ACCGGACCAG 
GACTTCCAAA GCCAGATGGA TGAGAATGGG ATCATTGGTC TGGCAGAGAG GCAAGGGCAG 
CAGGAGAGAG AAGAGGAGGA GGTTGCAGAG GAGCTGTTGT GGGATGTGAA GACCCCTGAG 
CCTGAGCATG GGCCGCCGCC TGCAGTAGAG GAGATAAGCT GTCATCTGAG CGAACTGCTG 
GACTCTGAAC CGCTCTCTCA GGCCACTGGA GACCACTACT ACTCTCCATC ACTGCAGGAA 
GAACACCAAA CAGACGATGA TCGTAGTGTG TTGTCTGAGG ATTGGGCTGA TGACGAGGGT 
GGTACAGGGA GCCCAGTAAA GCACAAATTA AGCTCTTTCC CTGAGAGAGA CACTGCATTA 
GACATGACCG ATGAGGAGAC AGAAGAGAAA AACACACAAG AAGATGCTCA GAGATTCATG 
GATCAATCAT TTCATGTCAC AGAGGAGAGG GCAGCTGTGT TTCAAACTCT GAAAACCCTT 
GATTTCAGTC AGGAAATGAG TGGGATTTTC AAAGATCATC CAATTTCAGG AGTCCCAGAG 
TACTCTGATC AAAGTGACTG CGAGGAACTT GATGCCCGAT TTCTTCCAGT CTCGCATCAT 
TTCTCTAGAA ACACTTCCAA TCCCACCAGT TTTCCCCATT TATCCTCTGA GGAACTGATG 
AATGACTGTG GGATTGAAGC TGAGACGTTA CCTGAGCTAG AAATTAGGTC AGATTCATCT 
CTTGGCACTT ACCAGAGTCC TGTCTCTAAA ACAAACCACA CTTTCAGATC AAACCAGGAA 
GAGATTAAAC ACAAGATCTC ACCTCAAACT TCACCCAGAA GCCCTGTAAT AAAAAACAGC 
CCTGAAAAAA TATACCAAGA TGCTACGAGT GGCGAAAAAT TAGATGGAAG GTTACACAGG 
CCTTACCACA ATCTGCAGCC TCCAACAGTC TCTCCCAGAA AAACAACCTC TGCTTCCTGC 
AACACTCAGG TTTCCATTCC AGCGCAGGCT ATTTCTAAAG CAAGCGTCTC CCCCTCAGAG 
CGATCGCCTC CTCCTACACC TCACAGAAAT TCAGCAATAA AGAATCACAG AAACTGCAAA 
ACCTCTCACA TGGATCCAGA TGACATCAGG AAAGGACAGC TGAATCATGC CTTGCCTGAT 
TTCTCCAAAG TAGAGCCGAA GGTGCGTTTC CCAAAAAGAA GTGAATACAA ACCTCCCAAA 
AGCCGAAGAC CCCCTCATGT TCGAACATCA TACCCGGGTC TTCCAGTGGT GTTCAAATCT 
CCTGCTGAGA TTGTGAAAGA GGTCCTGTTG AGCAGCTGTG ATGGGACTAC TGGGAGTCCA 
TCCTCCAGCG AGACTCAGAA GCACCTGCAC AATATCGTCC CAGAAGAGTT TCGCTGCCCA 
CAGCAGGCCA GCACACTGAT GCAGCAGCTA CAGGAGGACT ATAACAGGCT TCTTACCAAG 
TATGCAGAAG CTGAAAATAC TATTGATCGA CTTCGCCTTG AAGCAAAGGT GGGTCTCTCT 
TCGGAACCAC CCAAACCCAG TAGTACAGCT GTCCTGTCGG GAGTTATTCA GGAAGGGTCA 
AAGGTCATAA CCATCAGTTT TCCTCAAGCA CAGAGGGCAG AGTTCAGTAC AGGTTCTGTT 
CATCTGACCC AACAGAAGGA GCTCTCAGAA AACTCCAGAA AAGCAGCAAC TCGGCCTTCT 
TCTGTAAGCT CTATCAGCTC GAGGAGGTCT GGATCTCTTA CTCCAGACCA TCTGACAGAC 
ACTCTGTCCA AACAGAACCA AAGATTTCAG CTACAGGTGG ATGCATTTGA GTCGCTTTTA 
AAGGGTGGGA AGCTCAAGCC ATGTGAGCAG ACAAAGGGTC TCTCCACACT GGCTGAGGGG 
CAGGAGTCTC TTGAGAGGGC TTATCTAAAT GCACGAGTCC AATATCAGAT GCTGCAGCAA 
CGACAAGGCA GACATGCCAT CTTTGACCCA GACAGGGATC TAGAGGGTCA GATCTTCAGG 
TCAGGGATGC GATTAGAGGA GCTGAAAGAG TGGCTGGAAC AAGCAGAACA GAACCAACCT 
GTTTCTGAAC CTACTTTATC CCCATATCCT CCTTCAGATG TCCTTTCTGC ATCCATGCTG 
GAGCCTGAAC CAGTGCCTGA GATCCCCCTG TCAGCAGTCT GTCCTGAGGC TTGTGTTGGT 
GTGGAGGTCA GTTCAGTCAG TGGAGAGAGT GATAGTGGTA GAGAGGAAAT GGAAATGCTT 
CCGTTTGTTC TTCATCCCCT CGATAACAAA CACCAACGTG TGGAGAAAGA TTTCAGTGAA 
CTCATGGACT GCTATCAGAG TGTTAAAGAG CTCCCCGGGA CACTGGATCA GGCTGTGACT 
TTAAAGAGCC ATGACTTGCT GGATTTTGGA AGCTCTGTTC CTCCTGGGAA AAAAAGTGTA 
ACGGACGGAC AACCACTCAA GACTGTTAAT GAGAGAGAGA TGAGAAAACC AGCTCAGCAG 
CAGCCGGCAG CAGGTTGTGT CCCTCCCTCA TGCCCATCTG TCAGTTCACA GGTGTGCGAC 
TCTATGGAAA CTCACCCAGA GGCTTTTTGC CACTCCTATG TATTGGCAGG AGAATCGACC 
AGAGAGAGTA AAACAGCAGG AAACATGAAA GCTCAAAGCA CTAGCTTGAC CAGCCTGGAA 
GAGAGAACAC ACCACAGAAC TGTAGGCCTG AAAGCGAAGG CGAAGACTCT AAGAGCGCCC 
CCTATGGATG GGGTCATGTC TCCAGAAACA GACAGTGGCT TTATGGGCTC TGAAAGCAGT 
CAGCTCACTG CTGCATTACA CAGTCCTCTC CACCAGAGGG CAGTAATAAG TTCCATCAGA 
CTGTCTGACC CTCACACAAA GGACACAGAG AGACCAGAGT CTGCTTCTCC TTCTGGGCAG 
CCTTTCATTG TCTCTCCTTC TCCATCCCAA CCTTCTCCAA ACACCACAAA AAAGCACATT 
GATACATCAG GAAAATGCAC AGGTCCTTAT AAGAAAATAG GAGGGGAGGA AAGGTGTTCT 
TTTTCCAGTC TTTCTTCCTC CTTATCCCCC CTTCACTGGC CACGTAGCAG CATTCTGCCC 
TGGACAAGCA GCCTGACCAG TCAATCAGAG CGTGGAGCTG ATGAGGAAGA AGAAAAAGCA 
CAGAAAGACA AATACCCACA ACCGACCAAT CAGCAACTCC GCTCTCACAG AAGCCCCTCC 
CTCCACAGGC CTTATCACCA CAGTTCTGCT CAGCTAACAA ATCACCAAAA GGCTCTTCAA 
TCTCTGCAAA AAGAAGTGAA CCGATTGAAA GAGAGATTAG AGGGAAATCT GAGACTATCA 
AAACCTGCCA GACCTATGAG AACAGAAGTT TCTGCCTCTG AAGACACCAG AGGTCACACA 
CATCCACAGA CATCAACACC TCAGAGGCAT GATTTAAGGT CTTCAAAGAG GAGGAGAAGG 
CAGACAGAGA GAGCTGGCAA TGGAGAAAAA GAGAGAGGAA TTTCACCTAG ACCAGTCCTC 
AGACAAAGAT CAGCATCTTT ACCTCGACAT CGGCCTGAAA CTGATATGAC ATCTGATTCT 
GAGCATGCTC AATCTGAGCC AAGACCATCA ACGTCTAGAT ATGTCCCAGT GTCGCCTGTG 
AAATCAAGAG GAAGAAGACA AACCAGGAGA TTTGAATGTC ACAAAGAGGA TAGTACATCA 
GGCAGATCTG ACAACAGCGA TGAAGCAGAA CCAAACGGAG GAGCAGAATC TCTGTGTCCT 
GATTGCATAT CCGATCGTAC CAGTGCTTTG ACACGTCTCC TGAGGAGCAG GAGCTCTCCA 
CATCTTGCAC AGACTTCCTG TAGCCATTGC CCATTGTGTG GTGCATTACA AGCCATTCAA 
AGAGCCTCTA AGCCAGCTCC AGTCTCCAGT CCCAGAGCGA GTCAGCCAAT GAGATCCTTA 
CAGAGAGAAA AAGGAGGCGT GAACGTGACA GTGGCTCCTC CCCCTTTATT GCAGAGAGCT 
GTTCCAGTTG TCCCATATGT GCCTGTATAT CCTTCAACTC TATATTTCTC CAGCCCAATG 
ACTCCACCGG CATACCCACA ACCCCTCAAC ATCTCATCAA ATCCCGTCAG GCCTGAGAGG 
TCAGGGGTCA GAGGTCACCG CAGACGCTCT TCCTCCATGG AGTATCATCA GGATTCTATG 
AGCAGCGCTT TGACCCGTGC CATCACTGCA GCGAGACACA TGAGAGAAGC CTCACAGCGC 
ATGGCCAGCA GCCTCCACAC TGCATCCTGC AGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293532.1
CDS	259..4617
Translation	MERSRGGTSTGVLVWTPAPQLSSPSASEGSDFSWDEEPDQDFQSQMDENGIIGLAERQGQQEREEEEVAEELLWDVKTPEPEHGPPPAVEEISCHLSELLDSEPLSQATGDHYYSPSLQEEHQTDDDRSVLSEDWADDEGGTGSPVKHKLSSFPERDTALDMTDEETEEKNTQEDAQRFMDQSFHVTEERAAVFQTLKTLDFSQEMSGIFKDHPISGVPEYSDQSDCEELDARFLPVSHHFSRNTSNPTSFPHLSSEELMNDCGIEAETLPELEIRSDSSLGTYQSPVSKTNHTFRSNQEEIKHKISPQTSPRSPVIKNSPEKIYQDATSGEKLDGRLHRPYHNLQPPTVSPRKTTSASCNTQVSIPAQAISKASVSPSERSPPPTPHRNSAIKNHRNCKTSHMDPDDIRKGQLNHALPDFSKVEPKVRFPKRSEYKPPKSRRPPHVRTSYPGLPVVFKSPAEIVKEVLLSSCDGTTGSPSSSETQKHLHNIVPEEFRCPQQASTLMQQLQEDYNRLLTKYAEAENTIDRLRLEAKVGLSSEPPKPSSTAVLSGVIQEGSKVITISFPQAQRAEFSTGSVHLTQQKELSENSRKAATRPSSVSSISSRRSGSLTPDHLTDTLSKQNQRFQLQVDAFESLLKGGKLKPCEQTKGLSTLAEGQESLERAYLNARVQYQMLQQRQGRHAIFDPDRDLEGQIFRSGMRLEELKEWLEQAEQNQPVSEPTLSPYPPSDVLSASMLEPEPVPEIPLSAVCPEACVGVEVSSVSGESDSGREEMEMLPFVLHPLDNKHQRVEKDFSELMDCYQSVKELPGTLDQAVTLKSHDLLDFGSSVPPGKKSVTDGQPLKTVNEREMRKPAQQQPAAGCVPPSCPSVSSQVCDSMETHPEAFCHSYVLAGESTRESKTAGNMKAQSTSLTSLEERTHHRTVGLKAKAKTLRAPPMDGVMSPETDSGFMGSESSQLTAALHSPLHQRAVISSIRLSDPHTKDTERPESASPSGQPFIVSPSPSQPSPNTTKKHIDTSGKCTGPYKKIGGEERCSFSSLSSSLSPLHWPRSSILPWTSSLTSQSERGADEEEEKAQKDKYPQPTNQQLRSHRSPSLHRPYHHSSAQLTNHQKALQSLQKEVNRLKERLEGNLRLSKPARPMRTEVSASEDTRGHTHPQTSTPQRHDLRSSKRRRRQTERAGNGEKERGISPRPVLRQRSASLPRHRPETDMTSDSEHAQSEPRPSTSRYVPVSPVKSRGRRQTRRFECHKEDSTSGRSDNSDEAEPNGGAESLCPDCISDRTSALTRLLRSRSSPHLAQTSCSHCPLCGALQAIQRASKPAPVSSPRASQPMRSLQREKGGVNVTVAPPPLLQRAVPVVPYVPVYPSTLYFSSPMTPPAYPQPLNISSNPVRPERSGVRGHRRRSSSMEYHQDSMSSALTRAITAARHMREASQRMASSLHTASCSY

Target ORF information:

RefSeq Version	XM_009295257.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio AT-hook transcription factor (akna), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295257.2

ORF Insert Sequence:

ATGGAGCGGA GCAGAGGAGG CACCTCTACA GGGGTTCTGG TTTGGACACC TGCCCCACAG 
CTTTCCTCTC CCTCAGCCTC AGAGGGGTCA GACTTCAGCT GGGATGAAGA ACCGGACCAG 
GACTTCCAAA GCCAGATGGA TGAGAATGGG ATCATTGGTC TGGCAGAGAG GCAAGGGCAG 
CAGGAGAGAG AAGAGGAGGA GGTTGCAGAG GAGCTGTTGT GGGATGTGAA GACCCCTGAG 
CCTGAGCATG GGCCGCCGCC TGCAGTAGAG GAGATAAGCT GTCATCTGAG CGAACTGCTG 
GACTCTGAAC CGCTCTCTCA GGCCACTGGA GACCACTACT ACTCTCCATC ACTGCAGGAA 
GAACACCAAA CAGACGATGA TCGTAGTGTG TTGTCTGAGG ATTGGGCTGA TGACGAGGGT 
GGTACAGGGA GCCCAGTAAA GCACAAATTA AGCTCTTTCC CTGAGAGAGA CACTGCATTA 
GACATGACCG ATGAGGAGAC AGAAGAGAAA AACACACAAG AAGATGCTCA GAGATTCATG 
GATCAATCAT TTCATGTCAC AGAGGAGAGG GCAGCTGTGT TTCAAACTCT GAAAACCCTT 
GATTTCAGTC AGGAAATGAG TGGGATTTTC AAAGATCATC CAATTTCAGG AGTCCCAGAG 
TACTCTGATC AAAGTGACTG CGAGGAACTT GATGCCCGAT TTCTTCCAGT CTCGCATCAT 
TTCTCTAGAA ACACTTCCAA TCCCACCAGT TTTCCCCATT TATCCTCTGA GGAACTGATG 
AATGACTGTG GGATTGAAGC TGAGACGTTA CCTGAGCTAG AAATTAGGTC AGATTCATCT 
CTTGGCACTT ACCAGAGTCC TGTCTCTAAA ACAAACCACA CTTTCAGATC AAACCAGGAA 
GAGATTAAAC ACAAGATCTC ACCTCAAACT TCACCCAGAA GCCCTGTAAT AAAAAACAGC 
CCTGAAAAAA TATACCAAGA TGCTACGAGT GGCGAAAAAT TAGATGGAAG GTTACACAGG 
CCTTACCACA ATCTGCAGCC TCCAACAGTC TCTCCCAGAA AAACAACCTC TGCTTCCTGC 
AACACTCAGG TTTCCATTCC AGCGCAGGCT ATTTCTAAAG CAAGCGTCTC CCCCTCAGAG 
CGATCGCCTC CTCCTACACC TCACAGAAAT TCAGCAATAA AGAATCACAG AAACTGCAAA 
ACCTCTCACA TGGATCCAGA TGACATCAGG AAAGGACAGC TGAATCATGC CTTGCCTGAT 
TTCTCCAAAG TAGAGCCGAA GGTGCGTTTC CCAAAAAGAA GTGAATACAA ACCTCCCAAA 
AGCCGAAGAC CCCCTCATGT TCGAACATCA TACCCGGGTC TTCCAGTGGT GTTCAAATCT 
CCTGCTGAGA TTGTGAAAGA GGTCCTGTTG AGCAGCTGTG ATGGGACTAC TGGGAGTCCA 
TCCTCCAGCG AGACTCAGAA GCACCTGCAC AATATCGTCC CAGAAGAGTT TCGCTGCCCA 
CAGCAGGCCA GCACACTGAT GCAGCAGCTA CAGGAGGACT ATAACAGGCT TCTTACCAAG 
TATGCAGAAG CTGAAAATAC TATTGATCGA CTTCGCCTTG AAGCAAAGGT GGGTCTCTCT 
TCGGAACCAC CCAAACCCAG TAGTACAGCT GTCCTGTCGG GAGTTATTCA GGAAGGGTCA 
AAGGTCATAA CCATCAGTTT TCCTCAAGCA CAGAGGGCAG AGTTCAGTAC AGGTTCTGTT 
CATCTGACCC AACAGAAGGA GCTCTCAGAA AACTCCAGAA AAGCAGCAAC TCGGCCTTCT 
TCTGTAAGCT CTATCAGCTC GAGGAGGTCT GGATCTCTTA CTCCAGACCA TCTGACAGAC 
ACTCTGTCCA AACAGAACCA AAGATTTCAG CTACAGGTGG ATGCATTTGA GTCGCTTTTA 
AAGGGTGGGA AGCTCAAGCC ATGTGAGCAG ACAAAGGGTC TCTCCACACT GGCTGAGGGG 
CAGGAGTCTC TTGAGAGGGC TTATCTAAAT GCACGAGTCC AATATCAGAT GCTGCAGCAA 
CGACAAGGCA GACATGCCAT CTTTGACCCA GACAGGGATC TAGAGGGTCA GATCTTCAGG 
TCAGGGATGC GATTAGAGGA GCTGAAAGAG TGGCTGGAAC AAGCAGAACA GAACCAACCT 
GTTTCTGAAC CTACTTTATC CCCATATCCT CCTTCAGATG TCCTTTCTGC ATCCATGCTG 
GAGCCTGAAC CAGTGCCTGA GATCCCCCTG TCAGCAGTCT GTCCTGAGGC TTGTGTTGGT 
GTGGAGGTCA GTTCAGTCAG TGGAGAGAGT GATAGTGGTA GAGAGGAAAT GGAAATGCTT 
CCGTTTGTTC TTCATCCCCT CGATAACAAA CACCAACGTG TGGAGAAAGA TTTCAGTGAA 
CTCATGGACT GCTATCAGAG TGTTAAAGAG CTCCCCGGGA CACTGGATCA GGCTGTGACT 
TTAAAGAGCC ATGACTTGCT GGATTTTGGA AGCTCTGTTC CTCCTGGGAA AAAAAGTGTA 
ACGGACGGAC AACCACTCAA GACTGTTAAT GAGAGAGAGA TGAGAAAACC AGCTCAGCAG 
CAGCCGGCAG CAGGTTGTGT CCCTCCCTCA TGCCCATCTG TCAGTTCACA GGTGTGCGAC 
TCTATGGAAA CTCACCCAGA GGCTTTTTGC CACTCCTATG TATTGGCAGG AGAATCGACC 
AGAGAGAGTA AAACAGCAGG AAACATGAAA GCTCAAAGCA CTAGCTTGAC CAGCCTGGAA 
GAGAGAACAC ACCACAGAAC TGTAGGCCTG AAAGCGAAGG CGAAGACTCT AAGAGCGCCC 
CCTATGGATG GGGTCATGTC TCCAGAAACA GACAGTGGCT TTATGGGCTC TGAAAGCAGT 
CAGCTCACTG CTGCATTACA CAGTCCTCTC CACCAGAGGG CAGTAATAAG TTCCATCAGA 
CTGTCTGACC CTCACACAAA GGACACAGAG AGACCAGAGT CTGCTTCTCC TTCTGGGCAG 
CCTTTCATTG TCTCTCCTTC TCCATCCCAA CCTTCTCCAA ACACCACAAA AAAGCACATT 
GATACATCAG GAAAATGCAC AGGTCCTTAT AAGAAAATAG GAGGGGAGGA AAGGTGTTCT 
TTTTCCAGTC TTTCTTCCTC CTTATCCCCC CTTCACTGGC CACGTAGCAG CATTCTGCCC 
TGGACAAGCA GCCTGACCAG TCAATCAGAG CGTGGAGCTG ATGAGGAAGA AGAAAAAGCA 
CAGAAAGACA AATACCCACA ACCGACCAAT CAGCAACTCC GCTCTCACAG AAGCCCCTCC 
CTCCACAGGC CTTATCACCA CAGTTCTGCT CAGCTAACAA ATCACCAAAA GGCTCTTCAA 
TCTCTGCAAA AAGAAGTGAA CCGATTGAAA GAGAGATTAG AGGGAAATCT GAGACTATCA 
AAACCTGCCA GACCTATGAG AACAGAAGTT TCTGCCTCTG AAGACACCAG AGGTCACACA 
CATCCACAGA CATCAACACC TCAGAGGCAT GATTTAAGGT CTTCAAAGAG GAGGAGAAGG 
CAGACAGAGA GAGCTGGCAA TGGAGAAAAA GAGAGAGGAA TTTCACCTAG ACCAGTCCTC 
AGACAAAGAT CAGCATCTTT ACCTCGACAT CGGCCTGAAA CTGATATGAC ATCTGATTCT 
GAGCATGCTC AATCTGAGCC AAGACCATCA ACGTCTAGAT ATGTCCCAGT GTCGCCTGTG 
AAATCAAGAG GAAGAAGACA AACCAGGAGA TTTGAATGTC ACAAAGAGGA TAGTACATCA 
GGCAGATCTG ACAACAGCGA TGAAGCAGAA CCAAACGGAG GAGCAGAATC TCTGTGTCCT 
GATTGCATAT CCGATCGTAC CAGTGCTTTG ACACGTCTCC TGAGGAGCAG GAGCTCTCCA 
CATCTTGCAC AGACTTCCTG TAGCCATTGC CCATTGTGTG GTGCATTACA AGCCATTCAA 
AGAGCCTCTA AGCCAGCTCC AGTCTCCAGT CCCAGAGCGA GTCAGCCAAT GAGATCCTTA 
CAGAGAGAAA AAGGAGGCGT GAACGTGACA GTGGCTCCTC CCCCTTTATT GCAGAGAGCT 
GTTCCAGTTG TCCCATATGT GCCTGTATAT CCTTCAACTC TATATTTCTC CAGCCCAATG 
ACTCCACCGG CATACCCACA ACCCCTCAAC ATCTCATCAA ATCCCGTCAG GCCTGAGAGG 
TCAGGGGTCA GAGGTCACCG CAGACGCTCT TCCTCCATGG AGTATCATCA GGATTCTATG 
AGCAGCGCTT TGACCCGTGC CATCACTGCA GCGAGACACA TGAGAGAAGC CTCACAGCGC 
ATGGCCAGCA GCCTCCACAC TGCATCCTGC AGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

akna ( XM_678843.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_683935.4 Danio rerio AT-hook transcription factor (akna), transcript variant X1, mRNA.

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CloneID	ODa25697
Clone ID Related Accession (Same CDS sequence)	XM_009295258.3 , XM_009295258.2 , XM_009295256.3 , XM_005161116.4 , XM_678843.6 , XM_005161116.3 , XM_009295257.2 , XM_678843.7 , XM_017353013.1 , XM_009295256.2 , XM_009295257.3
Accession Version	XM_678843.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	AT-hook-containing transcription factor-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_678843.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGGA GCAGAGGAGG CACCTCTACA GGGGTTCTGG TTTGGACACC TGCCCCACAG 
CTTTCCTCTC CCTCAGCCTC AGAGGGGTCA GACTTCAGCT GGGATGAAGA ACCGGACCAG 
GACTTCCAAA GCCAGATGGA TGAGAATGGG ATCATTGGTC TGGCAGAGAG GCAAGGGCAG 
CAGGAGAGAG AAGAGGAGGA GGTTGCAGAG GAGCTGTTGT GGGATGTGAA GACCCCTGAG 
CCTGAGCATG GGCCGCCGCC TGCAGTAGAG GAGATAAGCT GTCATCTGAG CGAACTGCTG 
GACTCTGAAC CGCTCTCTCA GGCCACTGGA GACCACTACT ACTCTCCATC ACTGCAGGAA 
GAACACCAAA CAGACGATGA TCGTAGTGTG TTGTCTGAGG ATTGGGCTGA TGACGAGGGT 
GGTACAGGGA GCCCAGTAAA GCACAAATTA AGCTCTTTCC CTGAGAGAGA CACTGCATTA 
GACATGACCG ATGAGGAGAC AGAAGAGAAA AACACACAAG AAGATGCTCA GAGATTCATG 
GATCAATCAT TTCATGTCAC AGAGGAGAGG GCAGCTGTGT TTCAAACTCT GAAAACCCTT 
GATTTCAGTC AGGAAATGAG TGGGATTTTC AAAGATCATC CAATTTCAGG AGTCCCAGAG 
TACTCTGATC AAAGTGACTG CGAGGAACTT GATGCCCGAT TTCTTCCAGT CTCGCATCAT 
TTCTCTAGAA ACACTTCCAA TCCCACCAGT TTTCCCCATT TATCCTCTGA GGAACTGATG 
AATGACTGTG GGATTGAAGC TGAGACGTTA CCTGAGCTAG AAATTAGGTC AGATTCATCT 
CTTGGCACTT ACCAGAGTCC TGTCTCTAAA ACAAACCACA CTTTCAGATC AAACCAGGAA 
GAGATTAAAC ACAAGATCTC ACCTCAAACT TCACCCAGAA GCCCTGTAAT AAAAAACAGC 
CCTGAAAAAA TATACCAAGA TGCTACGAGT GGCGAAAAAT TAGATGGAAG GTTACACAGG 
CCTTACCACA ATCTGCAGCC TCCAACAGTC TCTCCCAGAA AAACAACCTC TGCTTCCTGC 
AACACTCAGG TTTCCATTCC AGCGCAGGCT ATTTCTAAAG CAAGCGTCTC CCCCTCAGAG 
CGATCGCCTC CTCCTACACC TCACAGAAAT TCAGCAATAA AGAATCACAG AAACTGCAAA 
ACCTCTCACA TGGATCCAGA TGACATCAGG AAAGGACAGC TGAATCATGC CTTGCCTGAT 
TTCTCCAAAG TAGAGCCGAA GGTGCGTTTC CCAAAAAGAA GTGAATACAA ACCTCCCAAA 
AGCCGAAGAC CCCCTCATGT TCGAACATCA TACCCGGGTC TTCCAGTGGT GTTCAAATCT 
CCTGCTGAGA TTGTGAAAGA GGTCCTGTTG AGCAGCTGTG ATGGGACTAC TGGGAGTCCA 
TCCTCCAGCG AGACTCAGAA GCACCTGCAC AATATCGTCC CAGAAGAGTT TCGCTGCCCA 
CAGCAGGCCA GCACACTGAT GCAGCAGCTA CAGGAGGACT ATAACAGGCT TCTTACCAAG 
TATGCAGAAG CTGAAAATAC TATTGATCGA CTTCGCCTTG AAGCAAAGGT GGGTCTCTCT 
TCGGAACCAC CCAAACCCAG TAGTACAGCT GTCCTGTCGG GAGTTATTCA GGAAGGGTCA 
AAGGTCATAA CCATCAGTTT TCCTCAAGCA CAGAGGGCAG AGTTCAGTAC AGGTTCTGTT 
CATCTGACCC AACAGAAGGA GCTCTCAGAA AACTCCAGAA AAGCAGCAAC TCGGCCTTCT 
TCTGTAAGCT CTATCAGCTC GAGGAGGTCT GGATCTCTTA CTCCAGACCA TCTGACAGAC 
ACTCTGTCCA AACAGAACCA AAGATTTCAG CTACAGGTGG ATGCATTTGA GTCGCTTTTA 
AAGGGTGGGA AGCTCAAGCC ATGTGAGCAG ACAAAGGGTC TCTCCACACT GGCTGAGGGG 
CAGGAGTCTC TTGAGAGGGC TTATCTAAAT GCACGAGTCC AATATCAGAT GCTGCAGCAA 
CGACAAGGCA GACATGCCAT CTTTGACCCA GACAGGGATC TAGAGGGTCA GATCTTCAGG 
TCAGGGATGC GATTAGAGGA GCTGAAAGAG TGGCTGGAAC AAGCAGAACA GAACCAACCT 
GTTTCTGAAC CTACTTTATC CCCATATCCT CCTTCAGATG TCCTTTCTGC ATCCATGCTG 
GAGCCTGAAC CAGTGCCTGA GATCCCCCTG TCAGCAGTCT GTCCTGAGGC TTGTGTTGGT 
GTGGAGGTCA GTTCAGTCAG TGGAGAGAGT GATAGTGGTA GAGAGGAAAT GGAAATGCTT 
CCGTTTGTTC TTCATCCCCT CGATAACAAA CACCAACGTG TGGAGAAAGA TTTCAGTGAA 
CTCATGGACT GCTATCAGAG TGTTAAAGAG CTCCCCGGGA CACTGGATCA GGCTGTGACT 
TTAAAGAGCC ATGACTTGCT GGATTTTGGA AGCTCTGTTC CTCCTGGGAA AAAAAGTGTA 
ACGGACGGAC AACCACTCAA GACTGTTAAT GAGAGAGAGA TGAGAAAACC AGCTCAGCAG 
CAGCCGGCAG CAGGTTGTGT CCCTCCCTCA TGCCCATCTG TCAGTTCACA GGTGTGCGAC 
TCTATGGAAA CTCACCCAGA GGCTTTTTGC CACTCCTATG TATTGGCAGG AGAATCGACC 
AGAGAGAGTA AAACAGCAGG AAACATGAAA GCTCAAAGCA CTAGCTTGAC CAGCCTGGAA 
GAGAGAACAC ACCACAGAAC TGTAGGCCTG AAAGCGAAGG CGAAGACTCT AAGAGCGCCC 
CCTATGGATG GGGTCATGTC TCCAGAAACA GACAGTGGCT TTATGGGCTC TGAAAGCAGT 
CAGCTCACTG CTGCATTACA CAGTCCTCTC CACCAGAGGG CAGTAATAAG TTCCATCAGA 
CTGTCTGACC CTCACACAAA GGACACAGAG AGACCAGAGT CTGCTTCTCC TTCTGGGCAG 
CCTTTCATTG TCTCTCCTTC TCCATCCCAA CCTTCTCCAA ACACCACAAA AAAGCACATT 
GATACATCAG GAAAATGCAC AGGTCCTTAT AAGAAAATAG GAGGGGAGGA AAGGTGTTCT 
TTTTCCAGTC TTTCTTCCTC CTTATCCCCC CTTCACTGGC CACGTAGCAG CATTCTGCCC 
TGGACAAGCA GCCTGACCAG TCAATCAGAG CGTGGAGCTG ATGAGGAAGA AGAAAAAGCA 
CAGAAAGACA AATACCCACA ACCGACCAAT CAGCAACTCC GCTCTCACAG AAGCCCCTCC 
CTCCACAGGC CTTATCACCA CAGTTCTGCT CAGCTAACAA ATCACCAAAA GGCTCTTCAA 
TCTCTGCAAA AAGAAGTGAA CCGATTGAAA GAGAGATTAG AGGGAAATCT GAGACTATCA 
AAACCTGCCA GACCTATGAG AACAGAAGTT TCTGCCTCTG AAGACACCAG AGGTCACACA 
CATCCACAGA CATCAACACC TCAGAGGCAT GATTTAAGGT CTTCAAAGAG GAGGAGAAGG 
CAGACAGAGA GAGCTGGCAA TGGAGAAAAA GAGAGAGGAA TTTCACCTAG ACCAGTCCTC 
AGACAAAGAT CAGCATCTTT ACCTCGACAT CGGCCTGAAA CTGATATGAC ATCTGATTCT 
GAGCATGCTC AATCTGAGCC AAGACCATCA ACGTCTAGAT ATGTCCCAGT GTCGCCTGTG 
AAATCAAGAG GAAGAAGACA AACCAGGAGA TTTGAATGTC ACAAAGAGGA TAGTACATCA 
GGCAGATCTG ACAACAGCGA TGAAGCAGAA CCAAACGGAG GAGCAGAATC TCTGTGTCCT 
GATTGCATAT CCGATCGTAC CAGTGCTTTG ACACGTCTCC TGAGGAGCAG GAGCTCTCCA 
CATCTTGCAC AGACTTCCTG TAGCCATTGC CCATTGTGTG GTGCATTACA AGCCATTCAA 
AGAGCCTCTA AGCCAGCTCC AGTCTCCAGT CCCAGAGCGA GTCAGCCAAT GAGATCCTTA 
CAGAGAGAAA AAGGAGGCGT GAACGTGACA GTGGCTCCTC CCCCTTTATT GCAGAGAGCT 
GTTCCAGTTG TCCCATATGT GCCTGTATAT CCTTCAACTC TATATTTCTC CAGCCCAATG 
ACTCCACCGG CATACCCACA ACCCCTCAAC ATCTCATCAA ATCCCGTCAG GCCTGAGAGG 
TCAGGGGTCA GAGGTCACCG CAGACGCTCT TCCTCCATGG AGTATCATCA GGATTCTATG 
AGCAGCGCTT TGACCCGTGC CATCACTGCA GCGAGACACA TGAGAGAAGC CTCACAGCGC 
ATGGCCAGCA GCCTCCACAC TGCATCCTGC AGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_683935.4
CDS	614..4972
Translation	MERSRGGTSTGVLVWTPAPQLSSPSASEGSDFSWDEEPDQDFQSQMDENGIIGLAERQGQQEREEEEVAEELLWDVKTPEPEHGPPPAVEEISCHLSELLDSEPLSQATGDHYYSPSLQEEHQTDDDRSVLSEDWADDEGGTGSPVKHKLSSFPERDTALDMTDEETEEKNTQEDAQRFMDQSFHVTEERAAVFQTLKTLDFSQEMSGIFKDHPISGVPEYSDQSDCEELDARFLPVSHHFSRNTSNPTSFPHLSSEELMNDCGIEAETLPELEIRSDSSLGTYQSPVSKTNHTFRSNQEEIKHKISPQTSPRSPVIKNSPEKIYQDATSGEKLDGRLHRPYHNLQPPTVSPRKTTSASCNTQVSIPAQAISKASVSPSERSPPPTPHRNSAIKNHRNCKTSHMDPDDIRKGQLNHALPDFSKVEPKVRFPKRSEYKPPKSRRPPHVRTSYPGLPVVFKSPAEIVKEVLLSSCDGTTGSPSSSETQKHLHNIVPEEFRCPQQASTLMQQLQEDYNRLLTKYAEAENTIDRLRLEAKVGLSSEPPKPSSTAVLSGVIQEGSKVITISFPQAQRAEFSTGSVHLTQQKELSENSRKAATRPSSVSSISSRRSGSLTPDHLTDTLSKQNQRFQLQVDAFESLLKGGKLKPCEQTKGLSTLAEGQESLERAYLNARVQYQMLQQRQGRHAIFDPDRDLEGQIFRSGMRLEELKEWLEQAEQNQPVSEPTLSPYPPSDVLSASMLEPEPVPEIPLSAVCPEACVGVEVSSVSGESDSGREEMEMLPFVLHPLDNKHQRVEKDFSELMDCYQSVKELPGTLDQAVTLKSHDLLDFGSSVPPGKKSVTDGQPLKTVNEREMRKPAQQQPAAGCVPPSCPSVSSQVCDSMETHPEAFCHSYVLAGESTRESKTAGNMKAQSTSLTSLEERTHHRTVGLKAKAKTLRAPPMDGVMSPETDSGFMGSESSQLTAALHSPLHQRAVISSIRLSDPHTKDTERPESASPSGQPFIVSPSPSQPSPNTTKKHIDTSGKCTGPYKKIGGEERCSFSSLSSSLSPLHWPRSSILPWTSSLTSQSERGADEEEEKAQKDKYPQPTNQQLRSHRSPSLHRPYHHSSAQLTNHQKALQSLQKEVNRLKERLEGNLRLSKPARPMRTEVSASEDTRGHTHPQTSTPQRHDLRSSKRRRRQTERAGNGEKERGISPRPVLRQRSASLPRHRPETDMTSDSEHAQSEPRPSTSRYVPVSPVKSRGRRQTRRFECHKEDSTSGRSDNSDEAEPNGGAESLCPDCISDRTSALTRLLRSRSSPHLAQTSCSHCPLCGALQAIQRASKPAPVSSPRASQPMRSLQREKGGVNVTVAPPPLLQRAVPVVPYVPVYPSTLYFSSPMTPPAYPQPLNISSNPVRPERSGVRGHRRRSSSMEYHQDSMSSALTRAITAARHMREASQRMASSLHTASCSY

Target ORF information:

RefSeq Version	XM_678843.7
Organism	Danio rerio(zebrafish)
Definition	Danio rerio AT-hook transcription factor (akna), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_678843.7

ORF Insert Sequence:

ATGGAGCGGA GCAGAGGAGG CACCTCTACA GGGGTTCTGG TTTGGACACC TGCCCCACAG 
CTTTCCTCTC CCTCAGCCTC AGAGGGGTCA GACTTCAGCT GGGATGAAGA ACCGGACCAG 
GACTTCCAAA GCCAGATGGA TGAGAATGGG ATCATTGGTC TGGCAGAGAG GCAAGGGCAG 
CAGGAGAGAG AAGAGGAGGA GGTTGCAGAG GAGCTGTTGT GGGATGTGAA GACCCCTGAG 
CCTGAGCATG GGCCGCCGCC TGCAGTAGAG GAGATAAGCT GTCATCTGAG CGAACTGCTG 
GACTCTGAAC CGCTCTCTCA GGCCACTGGA GACCACTACT ACTCTCCATC ACTGCAGGAA 
GAACACCAAA CAGACGATGA TCGTAGTGTG TTGTCTGAGG ATTGGGCTGA TGACGAGGGT 
GGTACAGGGA GCCCAGTAAA GCACAAATTA AGCTCTTTCC CTGAGAGAGA CACTGCATTA 
GACATGACCG ATGAGGAGAC AGAAGAGAAA AACACACAAG AAGATGCTCA GAGATTCATG 
GATCAATCAT TTCATGTCAC AGAGGAGAGG GCAGCTGTGT TTCAAACTCT GAAAACCCTT 
GATTTCAGTC AGGAAATGAG TGGGATTTTC AAAGATCATC CAATTTCAGG AGTCCCAGAG 
TACTCTGATC AAAGTGACTG CGAGGAACTT GATGCCCGAT TTCTTCCAGT CTCGCATCAT 
TTCTCTAGAA ACACTTCCAA TCCCACCAGT TTTCCCCATT TATCCTCTGA GGAACTGATG 
AATGACTGTG GGATTGAAGC TGAGACGTTA CCTGAGCTAG AAATTAGGTC AGATTCATCT 
CTTGGCACTT ACCAGAGTCC TGTCTCTAAA ACAAACCACA CTTTCAGATC AAACCAGGAA 
GAGATTAAAC ACAAGATCTC ACCTCAAACT TCACCCAGAA GCCCTGTAAT AAAAAACAGC 
CCTGAAAAAA TATACCAAGA TGCTACGAGT GGCGAAAAAT TAGATGGAAG GTTACACAGG 
CCTTACCACA ATCTGCAGCC TCCAACAGTC TCTCCCAGAA AAACAACCTC TGCTTCCTGC 
AACACTCAGG TTTCCATTCC AGCGCAGGCT ATTTCTAAAG CAAGCGTCTC CCCCTCAGAG 
CGATCGCCTC CTCCTACACC TCACAGAAAT TCAGCAATAA AGAATCACAG AAACTGCAAA 
ACCTCTCACA TGGATCCAGA TGACATCAGG AAAGGACAGC TGAATCATGC CTTGCCTGAT 
TTCTCCAAAG TAGAGCCGAA GGTGCGTTTC CCAAAAAGAA GTGAATACAA ACCTCCCAAA 
AGCCGAAGAC CCCCTCATGT TCGAACATCA TACCCGGGTC TTCCAGTGGT GTTCAAATCT 
CCTGCTGAGA TTGTGAAAGA GGTCCTGTTG AGCAGCTGTG ATGGGACTAC TGGGAGTCCA 
TCCTCCAGCG AGACTCAGAA GCACCTGCAC AATATCGTCC CAGAAGAGTT TCGCTGCCCA 
CAGCAGGCCA GCACACTGAT GCAGCAGCTA CAGGAGGACT ATAACAGGCT TCTTACCAAG 
TATGCAGAAG CTGAAAATAC TATTGATCGA CTTCGCCTTG AAGCAAAGGT GGGTCTCTCT 
TCGGAACCAC CCAAACCCAG TAGTACAGCT GTCCTGTCGG GAGTTATTCA GGAAGGGTCA 
AAGGTCATAA CCATCAGTTT TCCTCAAGCA CAGAGGGCAG AGTTCAGTAC AGGTTCTGTT 
CATCTGACCC AACAGAAGGA GCTCTCAGAA AACTCCAGAA AAGCAGCAAC TCGGCCTTCT 
TCTGTAAGCT CTATCAGCTC GAGGAGGTCT GGATCTCTTA CTCCAGACCA TCTGACAGAC 
ACTCTGTCCA AACAGAACCA AAGATTTCAG CTACAGGTGG ATGCATTTGA GTCGCTTTTA 
AAGGGTGGGA AGCTCAAGCC ATGTGAGCAG ACAAAGGGTC TCTCCACACT GGCTGAGGGG 
CAGGAGTCTC TTGAGAGGGC TTATCTAAAT GCACGAGTCC AATATCAGAT GCTGCAGCAA 
CGACAAGGCA GACATGCCAT CTTTGACCCA GACAGGGATC TAGAGGGTCA GATCTTCAGG 
TCAGGGATGC GATTAGAGGA GCTGAAAGAG TGGCTGGAAC AAGCAGAACA GAACCAACCT 
GTTTCTGAAC CTACTTTATC CCCATATCCT CCTTCAGATG TCCTTTCTGC ATCCATGCTG 
GAGCCTGAAC CAGTGCCTGA GATCCCCCTG TCAGCAGTCT GTCCTGAGGC TTGTGTTGGT 
GTGGAGGTCA GTTCAGTCAG TGGAGAGAGT GATAGTGGTA GAGAGGAAAT GGAAATGCTT 
CCGTTTGTTC TTCATCCCCT CGATAACAAA CACCAACGTG TGGAGAAAGA TTTCAGTGAA 
CTCATGGACT GCTATCAGAG TGTTAAAGAG CTCCCCGGGA CACTGGATCA GGCTGTGACT 
TTAAAGAGCC ATGACTTGCT GGATTTTGGA AGCTCTGTTC CTCCTGGGAA AAAAAGTGTA 
ACGGACGGAC AACCACTCAA GACTGTTAAT GAGAGAGAGA TGAGAAAACC AGCTCAGCAG 
CAGCCGGCAG CAGGTTGTGT CCCTCCCTCA TGCCCATCTG TCAGTTCACA GGTGTGCGAC 
TCTATGGAAA CTCACCCAGA GGCTTTTTGC CACTCCTATG TATTGGCAGG AGAATCGACC 
AGAGAGAGTA AAACAGCAGG AAACATGAAA GCTCAAAGCA CTAGCTTGAC CAGCCTGGAA 
GAGAGAACAC ACCACAGAAC TGTAGGCCTG AAAGCGAAGG CGAAGACTCT AAGAGCGCCC 
CCTATGGATG GGGTCATGTC TCCAGAAACA GACAGTGGCT TTATGGGCTC TGAAAGCAGT 
CAGCTCACTG CTGCATTACA CAGTCCTCTC CACCAGAGGG CAGTAATAAG TTCCATCAGA 
CTGTCTGACC CTCACACAAA GGACACAGAG AGACCAGAGT CTGCTTCTCC TTCTGGGCAG 
CCTTTCATTG TCTCTCCTTC TCCATCCCAA CCTTCTCCAA ACACCACAAA AAAGCACATT 
GATACATCAG GAAAATGCAC AGGTCCTTAT AAGAAAATAG GAGGGGAGGA AAGGTGTTCT 
TTTTCCAGTC TTTCTTCCTC CTTATCCCCC CTTCACTGGC CACGTAGCAG CATTCTGCCC 
TGGACAAGCA GCCTGACCAG TCAATCAGAG CGTGGAGCTG ATGAGGAAGA AGAAAAAGCA 
CAGAAAGACA AATACCCACA ACCGACCAAT CAGCAACTCC GCTCTCACAG AAGCCCCTCC 
CTCCACAGGC CTTATCACCA CAGTTCTGCT CAGCTAACAA ATCACCAAAA GGCTCTTCAA 
TCTCTGCAAA AAGAAGTGAA CCGATTGAAA GAGAGATTAG AGGGAAATCT GAGACTATCA 
AAACCTGCCA GACCTATGAG AACAGAAGTT TCTGCCTCTG AAGACACCAG AGGTCACACA 
CATCCACAGA CATCAACACC TCAGAGGCAT GATTTAAGGT CTTCAAAGAG GAGGAGAAGG 
CAGACAGAGA GAGCTGGCAA TGGAGAAAAA GAGAGAGGAA TTTCACCTAG ACCAGTCCTC 
AGACAAAGAT CAGCATCTTT ACCTCGACAT CGGCCTGAAA CTGATATGAC ATCTGATTCT 
GAGCATGCTC AATCTGAGCC AAGACCATCA ACGTCTAGAT ATGTCCCAGT GTCGCCTGTG 
AAATCAAGAG GAAGAAGACA AACCAGGAGA TTTGAATGTC ACAAAGAGGA TAGTACATCA 
GGCAGATCTG ACAACAGCGA TGAAGCAGAA CCAAACGGAG GAGCAGAATC TCTGTGTCCT 
GATTGCATAT CCGATCGTAC CAGTGCTTTG ACACGTCTCC TGAGGAGCAG GAGCTCTCCA 
CATCTTGCAC AGACTTCCTG TAGCCATTGC CCATTGTGTG GTGCATTACA AGCCATTCAA 
AGAGCCTCTA AGCCAGCTCC AGTCTCCAGT CCCAGAGCGA GTCAGCCAAT GAGATCCTTA 
CAGAGAGAAA AAGGAGGCGT GAACGTGACA GTGGCTCCTC CCCCTTTATT GCAGAGAGCT 
GTTCCAGTTG TCCCATATGT GCCTGTATAT CCTTCAACTC TATATTTCTC CAGCCCAATG 
ACTCCACCGG CATACCCACA ACCCCTCAAC ATCTCATCAA ATCCCGTCAG GCCTGAGAGG 
TCAGGGGTCA GAGGTCACCG CAGACGCTCT TCCTCCATGG AGTATCATCA GGATTCTATG 
AGCAGCGCTT TGACCCGTGC CATCACTGCA GCGAGACACA TGAGAGAAGC CTCACAGCGC 
ATGGCCAGCA GCCTCCACAC TGCATCCTGC AGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

akna ( XM_017353013.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208502.1 Danio rerio AT-hook transcription factor (akna), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa25697
Clone ID Related Accession (Same CDS sequence)	XM_009295258.3 , XM_009295258.2 , XM_009295256.3 , XM_005161116.4 , XM_678843.6 , XM_005161116.3 , XM_009295257.2 , XM_678843.7 , XM_017353013.1 , XM_009295256.2 , XM_009295257.3
Accession Version	XM_017353013.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	AT-hook-containing transcription factor-like isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGGA GCAGAGGAGG CACCTCTACA GGGGTTCTGG TTTGGACACC TGCCCCACAG 
CTTTCCTCTC CCTCAGCCTC AGAGGGGTCA GACTTCAGCT GGGATGAAGA ACCGGACCAG 
GACTTCCAAA GCCAGATGGA TGAGAATGGG ATCATTGGTC TGGCAGAGAG GCAAGGGCAG 
CAGGAGAGAG AAGAGGAGGA GGTTGCAGAG GAGCTGTTGT GGGATGTGAA GACCCCTGAG 
CCTGAGCATG GGCCGCCGCC TGCAGTAGAG GAGATAAGCT GTCATCTGAG CGAACTGCTG 
GACTCTGAAC CGCTCTCTCA GGCCACTGGA GACCACTACT ACTCTCCATC ACTGCAGGAA 
GAACACCAAA CAGACGATGA TCGTAGTGTG TTGTCTGAGG ATTGGGCTGA TGACGAGGGT 
GGTACAGGGA GCCCAGTAAA GCACAAATTA AGCTCTTTCC CTGAGAGAGA CACTGCATTA 
GACATGACCG ATGAGGAGAC AGAAGAGAAA AACACACAAG AAGATGCTCA GAGATTCATG 
GATCAATCAT TTCATGTCAC AGAGGAGAGG GCAGCTGTGT TTCAAACTCT GAAAACCCTT 
GATTTCAGTC AGGAAATGAG TGGGATTTTC AAAGATCATC CAATTTCAGG AGTCCCAGAG 
TACTCTGATC AAAGTGACTG CGAGGAACTT GATGCCCGAT TTCTTCCAGT CTCGCATCAT 
TTCTCTAGAA ACACTTCCAA TCCCACCAGT TTTCCCCATT TATCCTCTGA GGAACTGATG 
AATGACTGTG GGATTGAAGC TGAGACGTTA CCTGAGCTAG AAATTAGGTC AGATTCATCT 
CTTGGCACTT ACCAGAGTCC TGTCTCTAAA ACAAACCACA CTTTCAGATC AAACCAGGAA 
GAGATTAAAC ACAAGATCTC ACCTCAAACT TCACCCAGAA GCCCTGTAAT AAAAAACAGC 
CCTGAAAAAA TATACCAAGA TGCTACGAGT GGCGAAAAAT TAGATGGAAG GTTACACAGG 
CCTTACCACA ATCTGCAGCC TCCAACAGTC TCTCCCAGAA AAACAACCTC TGCTTCCTGC 
AACACTCAGG TTTCCATTCC AGCGCAGGCT ATTTCTAAAG CAAGCGTCTC CCCCTCAGAG 
CGATCGCCTC CTCCTACACC TCACAGAAAT TCAGCAATAA AGAATCACAG AAACTGCAAA 
ACCTCTCACA TGGATCCAGA TGACATCAGG AAAGGACAGC TGAATCATGC CTTGCCTGAT 
TTCTCCAAAG TAGAGCCGAA GGTGCGTTTC CCAAAAAGAA GTGAATACAA ACCTCCCAAA 
AGCCGAAGAC CCCCTCATGT TCGAACATCA TACCCGGGTC TTCCAGTGGT GTTCAAATCT 
CCTGCTGAGA TTGTGAAAGA GGTCCTGTTG AGCAGCTGTG ATGGGACTAC TGGGAGTCCA 
TCCTCCAGCG AGACTCAGAA GCACCTGCAC AATATCGTCC CAGAAGAGTT TCGCTGCCCA 
CAGCAGGCCA GCACACTGAT GCAGCAGCTA CAGGAGGACT ATAACAGGCT TCTTACCAAG 
TATGCAGAAG CTGAAAATAC TATTGATCGA CTTCGCCTTG AAGCAAAGGT GGGTCTCTCT 
TCGGAACCAC CCAAACCCAG TAGTACAGCT GTCCTGTCGG GAGTTATTCA GGAAGGGTCA 
AAGGTCATAA CCATCAGTTT TCCTCAAGCA CAGAGGGCAG AGTTCAGTAC AGGTTCTGTT 
CATCTGACCC AACAGAAGGA GCTCTCAGAA AACTCCAGAA AAGCAGCAAC TCGGCCTTCT 
TCTGTAAGCT CTATCAGCTC GAGGAGGTCT GGATCTCTTA CTCCAGACCA TCTGACAGAC 
ACTCTGTCCA AACAGAACCA AAGATTTCAG CTACAGGTGG ATGCATTTGA GTCGCTTTTA 
AAGGGTGGGA AGCTCAAGCC ATGTGAGCAG ACAAAGGGTC TCTCCACACT GGCTGAGGGG 
CAGGAGTCTC TTGAGAGGGC TTATCTAAAT GCACGAGTCC AATATCAGAT GCTGCAGCAA 
CGACAAGGCA GACATGCCAT CTTTGACCCA GACAGGGATC TAGAGGGTCA GATCTTCAGG 
TCAGGGATGC GATTAGAGGA GCTGAAAGAG TGGCTGGAAC AAGCAGAACA GAACCAACCT 
GTTTCTGAAC CTACTTTATC CCCATATCCT CCTTCAGATG TCCTTTCTGC ATCCATGCTG 
GAGCCTGAAC CAGTGCCTGA GATCCCCCTG TCAGCAGTCT GTCCTGAGGC TTGTGTTGGT 
GTGGAGGTCA GTTCAGTCAG TGGAGAGAGT GATAGTGGTA GAGAGGAAAT GGAAATGCTT 
CCGTTTGTTC TTCATCCCCT CGATAACAAA CACCAACGTG TGGAGAAAGA TTTCAGTGAA 
CTCATGGACT GCTATCAGAG TGTTAAAGAG CTCCCCGGGA CACTGGATCA GGCTGTGACT 
TTAAAGAGCC ATGACTTGCT GGATTTTGGA AGCTCTGTTC CTCCTGGGAA AAAAAGTGTA 
ACGGACGGAC AACCACTCAA GACTGTTAAT GAGAGAGAGA TGAGAAAACC AGCTCAGCAG 
CAGCCGGCAG CAGGTTGTGT CCCTCCCTCA TGCCCATCTG TCAGTTCACA GGTGTGCGAC 
TCTATGGAAA CTCACCCAGA GGCTTTTTGC CACTCCTATG TATTGGCAGG AGAATCGACC 
AGAGAGAGTA AAACAGCAGG AAACATGAAA GCTCAAAGCA CTAGCTTGAC CAGCCTGGAA 
GAGAGAACAC ACCACAGAAC TGTAGGCCTG AAAGCGAAGG CGAAGACTCT AAGAGCGCCC 
CCTATGGATG GGGTCATGTC TCCAGAAACA GACAGTGGCT TTATGGGCTC TGAAAGCAGT 
CAGCTCACTG CTGCATTACA CAGTCCTCTC CACCAGAGGG CAGTAATAAG TTCCATCAGA 
CTGTCTGACC CTCACACAAA GGACACAGAG AGACCAGAGT CTGCTTCTCC TTCTGGGCAG 
CCTTTCATTG TCTCTCCTTC TCCATCCCAA CCTTCTCCAA ACACCACAAA AAAGCACATT 
GATACATCAG GAAAATGCAC AGGTCCTTAT AAGAAAATAG GAGGGGAGGA AAGGTGTTCT 
TTTTCCAGTC TTTCTTCCTC CTTATCCCCC CTTCACTGGC CACGTAGCAG CATTCTGCCC 
TGGACAAGCA GCCTGACCAG TCAATCAGAG CGTGGAGCTG ATGAGGAAGA AGAAAAAGCA 
CAGAAAGACA AATACCCACA ACCGACCAAT CAGCAACTCC GCTCTCACAG AAGCCCCTCC 
CTCCACAGGC CTTATCACCA CAGTTCTGCT CAGCTAACAA ATCACCAAAA GGCTCTTCAA 
TCTCTGCAAA AAGAAGTGAA CCGATTGAAA GAGAGATTAG AGGGAAATCT GAGACTATCA 
AAACCTGCCA GACCTATGAG AACAGAAGTT TCTGCCTCTG AAGACACCAG AGGTCACACA 
CATCCACAGA CATCAACACC TCAGAGGCAT GATTTAAGGT CTTCAAAGAG GAGGAGAAGG 
CAGACAGAGA GAGCTGGCAA TGGAGAAAAA GAGAGAGGAA TTTCACCTAG ACCAGTCCTC 
AGACAAAGAT CAGCATCTTT ACCTCGACAT CGGCCTGAAA CTGATATGAC ATCTGATTCT 
GAGCATGCTC AATCTGAGCC AAGACCATCA ACGTCTAGAT ATGTCCCAGT GTCGCCTGTG 
AAATCAAGAG GAAGAAGACA AACCAGGAGA TTTGAATGTC ACAAAGAGGA TAGTACATCA 
GGCAGATCTG ACAACAGCGA TGAAGCAGAA CCAAACGGAG GAGCAGAATC TCTGTGTCCT 
GATTGCATAT CCGATCGTAC CAGTGCTTTG ACACGTCTCC TGAGGAGCAG GAGCTCTCCA 
CATCTTGCAC AGACTTCCTG TAGCCATTGC CCATTGTGTG GTGCATTACA AGCCATTCAA 
AGAGCCTCTA AGCCAGCTCC AGTCTCCAGT CCCAGAGCGA GTCAGCCAAT GAGATCCTTA 
CAGAGAGAAA AAGGAGGCGT GAACGTGACA GTGGCTCCTC CCCCTTTATT GCAGAGAGCT 
GTTCCAGTTG TCCCATATGT GCCTGTATAT CCTTCAACTC TATATTTCTC CAGCCCAATG 
ACTCCACCGG CATACCCACA ACCCCTCAAC ATCTCATCAA ATCCCGTCAG GCCTGAGAGG 
TCAGGGGTCA GAGGTCACCG CAGACGCTCT TCCTCCATGG AGTATCATCA GGATTCTATG 
AGCAGCGCTT TGACCCGTGC CATCACTGCA GCGAGACACA TGAGAGAAGC CTCACAGCGC 
ATGGCCAGCA GCCTCCACAC TGCATCCTGC AGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208502.1
CDS	616..4974
Translation	MERSRGGTSTGVLVWTPAPQLSSPSASEGSDFSWDEEPDQDFQSQMDENGIIGLAERQGQQEREEEEVAEELLWDVKTPEPEHGPPPAVEEISCHLSELLDSEPLSQATGDHYYSPSLQEEHQTDDDRSVLSEDWADDEGGTGSPVKHKLSSFPERDTALDMTDEETEEKNTQEDAQRFMDQSFHVTEERAAVFQTLKTLDFSQEMSGIFKDHPISGVPEYSDQSDCEELDARFLPVSHHFSRNTSNPTSFPHLSSEELMNDCGIEAETLPELEIRSDSSLGTYQSPVSKTNHTFRSNQEEIKHKISPQTSPRSPVIKNSPEKIYQDATSGEKLDGRLHRPYHNLQPPTVSPRKTTSASCNTQVSIPAQAISKASVSPSERSPPPTPHRNSAIKNHRNCKTSHMDPDDIRKGQLNHALPDFSKVEPKVRFPKRSEYKPPKSRRPPHVRTSYPGLPVVFKSPAEIVKEVLLSSCDGTTGSPSSSETQKHLHNIVPEEFRCPQQASTLMQQLQEDYNRLLTKYAEAENTIDRLRLEAKVGLSSEPPKPSSTAVLSGVIQEGSKVITISFPQAQRAEFSTGSVHLTQQKELSENSRKAATRPSSVSSISSRRSGSLTPDHLTDTLSKQNQRFQLQVDAFESLLKGGKLKPCEQTKGLSTLAEGQESLERAYLNARVQYQMLQQRQGRHAIFDPDRDLEGQIFRSGMRLEELKEWLEQAEQNQPVSEPTLSPYPPSDVLSASMLEPEPVPEIPLSAVCPEACVGVEVSSVSGESDSGREEMEMLPFVLHPLDNKHQRVEKDFSELMDCYQSVKELPGTLDQAVTLKSHDLLDFGSSVPPGKKSVTDGQPLKTVNEREMRKPAQQQPAAGCVPPSCPSVSSQVCDSMETHPEAFCHSYVLAGESTRESKTAGNMKAQSTSLTSLEERTHHRTVGLKAKAKTLRAPPMDGVMSPETDSGFMGSESSQLTAALHSPLHQRAVISSIRLSDPHTKDTERPESASPSGQPFIVSPSPSQPSPNTTKKHIDTSGKCTGPYKKIGGEERCSFSSLSSSLSPLHWPRSSILPWTSSLTSQSERGADEEEEKAQKDKYPQPTNQQLRSHRSPSLHRPYHHSSAQLTNHQKALQSLQKEVNRLKERLEGNLRLSKPARPMRTEVSASEDTRGHTHPQTSTPQRHDLRSSKRRRRQTERAGNGEKERGISPRPVLRQRSASLPRHRPETDMTSDSEHAQSEPRPSTSRYVPVSPVKSRGRRQTRRFECHKEDSTSGRSDNSDEAEPNGGAESLCPDCISDRTSALTRLLRSRSSPHLAQTSCSHCPLCGALQAIQRASKPAPVSSPRASQPMRSLQREKGGVNVTVAPPPLLQRAVPVVPYVPVYPSTLYFSSPMTPPAYPQPLNISSNPVRPERSGVRGHRRRSSSMEYHQDSMSSALTRAITAARHMREASQRMASSLHTASCSY

Target ORF information:

RefSeq Version	XM_017353013.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio AT-hook transcription factor (akna), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353013.1

ORF Insert Sequence:

ATGGAGCGGA GCAGAGGAGG CACCTCTACA GGGGTTCTGG TTTGGACACC TGCCCCACAG 
CTTTCCTCTC CCTCAGCCTC AGAGGGGTCA GACTTCAGCT GGGATGAAGA ACCGGACCAG 
GACTTCCAAA GCCAGATGGA TGAGAATGGG ATCATTGGTC TGGCAGAGAG GCAAGGGCAG 
CAGGAGAGAG AAGAGGAGGA GGTTGCAGAG GAGCTGTTGT GGGATGTGAA GACCCCTGAG 
CCTGAGCATG GGCCGCCGCC TGCAGTAGAG GAGATAAGCT GTCATCTGAG CGAACTGCTG 
GACTCTGAAC CGCTCTCTCA GGCCACTGGA GACCACTACT ACTCTCCATC ACTGCAGGAA 
GAACACCAAA CAGACGATGA TCGTAGTGTG TTGTCTGAGG ATTGGGCTGA TGACGAGGGT 
GGTACAGGGA GCCCAGTAAA GCACAAATTA AGCTCTTTCC CTGAGAGAGA CACTGCATTA 
GACATGACCG ATGAGGAGAC AGAAGAGAAA AACACACAAG AAGATGCTCA GAGATTCATG 
GATCAATCAT TTCATGTCAC AGAGGAGAGG GCAGCTGTGT TTCAAACTCT GAAAACCCTT 
GATTTCAGTC AGGAAATGAG TGGGATTTTC AAAGATCATC CAATTTCAGG AGTCCCAGAG 
TACTCTGATC AAAGTGACTG CGAGGAACTT GATGCCCGAT TTCTTCCAGT CTCGCATCAT 
TTCTCTAGAA ACACTTCCAA TCCCACCAGT TTTCCCCATT TATCCTCTGA GGAACTGATG 
AATGACTGTG GGATTGAAGC TGAGACGTTA CCTGAGCTAG AAATTAGGTC AGATTCATCT 
CTTGGCACTT ACCAGAGTCC TGTCTCTAAA ACAAACCACA CTTTCAGATC AAACCAGGAA 
GAGATTAAAC ACAAGATCTC ACCTCAAACT TCACCCAGAA GCCCTGTAAT AAAAAACAGC 
CCTGAAAAAA TATACCAAGA TGCTACGAGT GGCGAAAAAT TAGATGGAAG GTTACACAGG 
CCTTACCACA ATCTGCAGCC TCCAACAGTC TCTCCCAGAA AAACAACCTC TGCTTCCTGC 
AACACTCAGG TTTCCATTCC AGCGCAGGCT ATTTCTAAAG CAAGCGTCTC CCCCTCAGAG 
CGATCGCCTC CTCCTACACC TCACAGAAAT TCAGCAATAA AGAATCACAG AAACTGCAAA 
ACCTCTCACA TGGATCCAGA TGACATCAGG AAAGGACAGC TGAATCATGC CTTGCCTGAT 
TTCTCCAAAG TAGAGCCGAA GGTGCGTTTC CCAAAAAGAA GTGAATACAA ACCTCCCAAA 
AGCCGAAGAC CCCCTCATGT TCGAACATCA TACCCGGGTC TTCCAGTGGT GTTCAAATCT 
CCTGCTGAGA TTGTGAAAGA GGTCCTGTTG AGCAGCTGTG ATGGGACTAC TGGGAGTCCA 
TCCTCCAGCG AGACTCAGAA GCACCTGCAC AATATCGTCC CAGAAGAGTT TCGCTGCCCA 
CAGCAGGCCA GCACACTGAT GCAGCAGCTA CAGGAGGACT ATAACAGGCT TCTTACCAAG 
TATGCAGAAG CTGAAAATAC TATTGATCGA CTTCGCCTTG AAGCAAAGGT GGGTCTCTCT 
TCGGAACCAC CCAAACCCAG TAGTACAGCT GTCCTGTCGG GAGTTATTCA GGAAGGGTCA 
AAGGTCATAA CCATCAGTTT TCCTCAAGCA CAGAGGGCAG AGTTCAGTAC AGGTTCTGTT 
CATCTGACCC AACAGAAGGA GCTCTCAGAA AACTCCAGAA AAGCAGCAAC TCGGCCTTCT 
TCTGTAAGCT CTATCAGCTC GAGGAGGTCT GGATCTCTTA CTCCAGACCA TCTGACAGAC 
ACTCTGTCCA AACAGAACCA AAGATTTCAG CTACAGGTGG ATGCATTTGA GTCGCTTTTA 
AAGGGTGGGA AGCTCAAGCC ATGTGAGCAG ACAAAGGGTC TCTCCACACT GGCTGAGGGG 
CAGGAGTCTC TTGAGAGGGC TTATCTAAAT GCACGAGTCC AATATCAGAT GCTGCAGCAA 
CGACAAGGCA GACATGCCAT CTTTGACCCA GACAGGGATC TAGAGGGTCA GATCTTCAGG 
TCAGGGATGC GATTAGAGGA GCTGAAAGAG TGGCTGGAAC AAGCAGAACA GAACCAACCT 
GTTTCTGAAC CTACTTTATC CCCATATCCT CCTTCAGATG TCCTTTCTGC ATCCATGCTG 
GAGCCTGAAC CAGTGCCTGA GATCCCCCTG TCAGCAGTCT GTCCTGAGGC TTGTGTTGGT 
GTGGAGGTCA GTTCAGTCAG TGGAGAGAGT GATAGTGGTA GAGAGGAAAT GGAAATGCTT 
CCGTTTGTTC TTCATCCCCT CGATAACAAA CACCAACGTG TGGAGAAAGA TTTCAGTGAA 
CTCATGGACT GCTATCAGAG TGTTAAAGAG CTCCCCGGGA CACTGGATCA GGCTGTGACT 
TTAAAGAGCC ATGACTTGCT GGATTTTGGA AGCTCTGTTC CTCCTGGGAA AAAAAGTGTA 
ACGGACGGAC AACCACTCAA GACTGTTAAT GAGAGAGAGA TGAGAAAACC AGCTCAGCAG 
CAGCCGGCAG CAGGTTGTGT CCCTCCCTCA TGCCCATCTG TCAGTTCACA GGTGTGCGAC 
TCTATGGAAA CTCACCCAGA GGCTTTTTGC CACTCCTATG TATTGGCAGG AGAATCGACC 
AGAGAGAGTA AAACAGCAGG AAACATGAAA GCTCAAAGCA CTAGCTTGAC CAGCCTGGAA 
GAGAGAACAC ACCACAGAAC TGTAGGCCTG AAAGCGAAGG CGAAGACTCT AAGAGCGCCC 
CCTATGGATG GGGTCATGTC TCCAGAAACA GACAGTGGCT TTATGGGCTC TGAAAGCAGT 
CAGCTCACTG CTGCATTACA CAGTCCTCTC CACCAGAGGG CAGTAATAAG TTCCATCAGA 
CTGTCTGACC CTCACACAAA GGACACAGAG AGACCAGAGT CTGCTTCTCC TTCTGGGCAG 
CCTTTCATTG TCTCTCCTTC TCCATCCCAA CCTTCTCCAA ACACCACAAA AAAGCACATT 
GATACATCAG GAAAATGCAC AGGTCCTTAT AAGAAAATAG GAGGGGAGGA AAGGTGTTCT 
TTTTCCAGTC TTTCTTCCTC CTTATCCCCC CTTCACTGGC CACGTAGCAG CATTCTGCCC 
TGGACAAGCA GCCTGACCAG TCAATCAGAG CGTGGAGCTG ATGAGGAAGA AGAAAAAGCA 
CAGAAAGACA AATACCCACA ACCGACCAAT CAGCAACTCC GCTCTCACAG AAGCCCCTCC 
CTCCACAGGC CTTATCACCA CAGTTCTGCT CAGCTAACAA ATCACCAAAA GGCTCTTCAA 
TCTCTGCAAA AAGAAGTGAA CCGATTGAAA GAGAGATTAG AGGGAAATCT GAGACTATCA 
AAACCTGCCA GACCTATGAG AACAGAAGTT TCTGCCTCTG AAGACACCAG AGGTCACACA 
CATCCACAGA CATCAACACC TCAGAGGCAT GATTTAAGGT CTTCAAAGAG GAGGAGAAGG 
CAGACAGAGA GAGCTGGCAA TGGAGAAAAA GAGAGAGGAA TTTCACCTAG ACCAGTCCTC 
AGACAAAGAT CAGCATCTTT ACCTCGACAT CGGCCTGAAA CTGATATGAC ATCTGATTCT 
GAGCATGCTC AATCTGAGCC AAGACCATCA ACGTCTAGAT ATGTCCCAGT GTCGCCTGTG 
AAATCAAGAG GAAGAAGACA AACCAGGAGA TTTGAATGTC ACAAAGAGGA TAGTACATCA 
GGCAGATCTG ACAACAGCGA TGAAGCAGAA CCAAACGGAG GAGCAGAATC TCTGTGTCCT 
GATTGCATAT CCGATCGTAC CAGTGCTTTG ACACGTCTCC TGAGGAGCAG GAGCTCTCCA 
CATCTTGCAC AGACTTCCTG TAGCCATTGC CCATTGTGTG GTGCATTACA AGCCATTCAA 
AGAGCCTCTA AGCCAGCTCC AGTCTCCAGT CCCAGAGCGA GTCAGCCAAT GAGATCCTTA 
CAGAGAGAAA AAGGAGGCGT GAACGTGACA GTGGCTCCTC CCCCTTTATT GCAGAGAGCT 
GTTCCAGTTG TCCCATATGT GCCTGTATAT CCTTCAACTC TATATTTCTC CAGCCCAATG 
ACTCCACCGG CATACCCACA ACCCCTCAAC ATCTCATCAA ATCCCGTCAG GCCTGAGAGG 
TCAGGGGTCA GAGGTCACCG CAGACGCTCT TCCTCCATGG AGTATCATCA GGATTCTATG 
AGCAGCGCTT TGACCCGTGC CATCACTGCA GCGAGACACA TGAGAGAAGC CTCACAGCGC 
ATGGCCAGCA GCCTCCACAC TGCATCCTGC AGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

akna ( XM_009295256.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293531.1 Danio rerio AT-hook transcription factor (akna), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa25697
Clone ID Related Accession (Same CDS sequence)	XM_009295258.3 , XM_009295258.2 , XM_009295256.3 , XM_005161116.4 , XM_678843.6 , XM_005161116.3 , XM_009295257.2 , XM_678843.7 , XM_017353013.1 , XM_009295256.2 , XM_009295257.3
Accession Version	XM_009295256.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	AT-hook-containing transcription factor-like isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295256.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGGA GCAGAGGAGG CACCTCTACA GGGGTTCTGG TTTGGACACC TGCCCCACAG 
CTTTCCTCTC CCTCAGCCTC AGAGGGGTCA GACTTCAGCT GGGATGAAGA ACCGGACCAG 
GACTTCCAAA GCCAGATGGA TGAGAATGGG ATCATTGGTC TGGCAGAGAG GCAAGGGCAG 
CAGGAGAGAG AAGAGGAGGA GGTTGCAGAG GAGCTGTTGT GGGATGTGAA GACCCCTGAG 
CCTGAGCATG GGCCGCCGCC TGCAGTAGAG GAGATAAGCT GTCATCTGAG CGAACTGCTG 
GACTCTGAAC CGCTCTCTCA GGCCACTGGA GACCACTACT ACTCTCCATC ACTGCAGGAA 
GAACACCAAA CAGACGATGA TCGTAGTGTG TTGTCTGAGG ATTGGGCTGA TGACGAGGGT 
GGTACAGGGA GCCCAGTAAA GCACAAATTA AGCTCTTTCC CTGAGAGAGA CACTGCATTA 
GACATGACCG ATGAGGAGAC AGAAGAGAAA AACACACAAG AAGATGCTCA GAGATTCATG 
GATCAATCAT TTCATGTCAC AGAGGAGAGG GCAGCTGTGT TTCAAACTCT GAAAACCCTT 
GATTTCAGTC AGGAAATGAG TGGGATTTTC AAAGATCATC CAATTTCAGG AGTCCCAGAG 
TACTCTGATC AAAGTGACTG CGAGGAACTT GATGCCCGAT TTCTTCCAGT CTCGCATCAT 
TTCTCTAGAA ACACTTCCAA TCCCACCAGT TTTCCCCATT TATCCTCTGA GGAACTGATG 
AATGACTGTG GGATTGAAGC TGAGACGTTA CCTGAGCTAG AAATTAGGTC AGATTCATCT 
CTTGGCACTT ACCAGAGTCC TGTCTCTAAA ACAAACCACA CTTTCAGATC AAACCAGGAA 
GAGATTAAAC ACAAGATCTC ACCTCAAACT TCACCCAGAA GCCCTGTAAT AAAAAACAGC 
CCTGAAAAAA TATACCAAGA TGCTACGAGT GGCGAAAAAT TAGATGGAAG GTTACACAGG 
CCTTACCACA ATCTGCAGCC TCCAACAGTC TCTCCCAGAA AAACAACCTC TGCTTCCTGC 
AACACTCAGG TTTCCATTCC AGCGCAGGCT ATTTCTAAAG CAAGCGTCTC CCCCTCAGAG 
CGATCGCCTC CTCCTACACC TCACAGAAAT TCAGCAATAA AGAATCACAG AAACTGCAAA 
ACCTCTCACA TGGATCCAGA TGACATCAGG AAAGGACAGC TGAATCATGC CTTGCCTGAT 
TTCTCCAAAG TAGAGCCGAA GGTGCGTTTC CCAAAAAGAA GTGAATACAA ACCTCCCAAA 
AGCCGAAGAC CCCCTCATGT TCGAACATCA TACCCGGGTC TTCCAGTGGT GTTCAAATCT 
CCTGCTGAGA TTGTGAAAGA GGTCCTGTTG AGCAGCTGTG ATGGGACTAC TGGGAGTCCA 
TCCTCCAGCG AGACTCAGAA GCACCTGCAC AATATCGTCC CAGAAGAGTT TCGCTGCCCA 
CAGCAGGCCA GCACACTGAT GCAGCAGCTA CAGGAGGACT ATAACAGGCT TCTTACCAAG 
TATGCAGAAG CTGAAAATAC TATTGATCGA CTTCGCCTTG AAGCAAAGGT GGGTCTCTCT 
TCGGAACCAC CCAAACCCAG TAGTACAGCT GTCCTGTCGG GAGTTATTCA GGAAGGGTCA 
AAGGTCATAA CCATCAGTTT TCCTCAAGCA CAGAGGGCAG AGTTCAGTAC AGGTTCTGTT 
CATCTGACCC AACAGAAGGA GCTCTCAGAA AACTCCAGAA AAGCAGCAAC TCGGCCTTCT 
TCTGTAAGCT CTATCAGCTC GAGGAGGTCT GGATCTCTTA CTCCAGACCA TCTGACAGAC 
ACTCTGTCCA AACAGAACCA AAGATTTCAG CTACAGGTGG ATGCATTTGA GTCGCTTTTA 
AAGGGTGGGA AGCTCAAGCC ATGTGAGCAG ACAAAGGGTC TCTCCACACT GGCTGAGGGG 
CAGGAGTCTC TTGAGAGGGC TTATCTAAAT GCACGAGTCC AATATCAGAT GCTGCAGCAA 
CGACAAGGCA GACATGCCAT CTTTGACCCA GACAGGGATC TAGAGGGTCA GATCTTCAGG 
TCAGGGATGC GATTAGAGGA GCTGAAAGAG TGGCTGGAAC AAGCAGAACA GAACCAACCT 
GTTTCTGAAC CTACTTTATC CCCATATCCT CCTTCAGATG TCCTTTCTGC ATCCATGCTG 
GAGCCTGAAC CAGTGCCTGA GATCCCCCTG TCAGCAGTCT GTCCTGAGGC TTGTGTTGGT 
GTGGAGGTCA GTTCAGTCAG TGGAGAGAGT GATAGTGGTA GAGAGGAAAT GGAAATGCTT 
CCGTTTGTTC TTCATCCCCT CGATAACAAA CACCAACGTG TGGAGAAAGA TTTCAGTGAA 
CTCATGGACT GCTATCAGAG TGTTAAAGAG CTCCCCGGGA CACTGGATCA GGCTGTGACT 
TTAAAGAGCC ATGACTTGCT GGATTTTGGA AGCTCTGTTC CTCCTGGGAA AAAAAGTGTA 
ACGGACGGAC AACCACTCAA GACTGTTAAT GAGAGAGAGA TGAGAAAACC AGCTCAGCAG 
CAGCCGGCAG CAGGTTGTGT CCCTCCCTCA TGCCCATCTG TCAGTTCACA GGTGTGCGAC 
TCTATGGAAA CTCACCCAGA GGCTTTTTGC CACTCCTATG TATTGGCAGG AGAATCGACC 
AGAGAGAGTA AAACAGCAGG AAACATGAAA GCTCAAAGCA CTAGCTTGAC CAGCCTGGAA 
GAGAGAACAC ACCACAGAAC TGTAGGCCTG AAAGCGAAGG CGAAGACTCT AAGAGCGCCC 
CCTATGGATG GGGTCATGTC TCCAGAAACA GACAGTGGCT TTATGGGCTC TGAAAGCAGT 
CAGCTCACTG CTGCATTACA CAGTCCTCTC CACCAGAGGG CAGTAATAAG TTCCATCAGA 
CTGTCTGACC CTCACACAAA GGACACAGAG AGACCAGAGT CTGCTTCTCC TTCTGGGCAG 
CCTTTCATTG TCTCTCCTTC TCCATCCCAA CCTTCTCCAA ACACCACAAA AAAGCACATT 
GATACATCAG GAAAATGCAC AGGTCCTTAT AAGAAAATAG GAGGGGAGGA AAGGTGTTCT 
TTTTCCAGTC TTTCTTCCTC CTTATCCCCC CTTCACTGGC CACGTAGCAG CATTCTGCCC 
TGGACAAGCA GCCTGACCAG TCAATCAGAG CGTGGAGCTG ATGAGGAAGA AGAAAAAGCA 
CAGAAAGACA AATACCCACA ACCGACCAAT CAGCAACTCC GCTCTCACAG AAGCCCCTCC 
CTCCACAGGC CTTATCACCA CAGTTCTGCT CAGCTAACAA ATCACCAAAA GGCTCTTCAA 
TCTCTGCAAA AAGAAGTGAA CCGATTGAAA GAGAGATTAG AGGGAAATCT GAGACTATCA 
AAACCTGCCA GACCTATGAG AACAGAAGTT TCTGCCTCTG AAGACACCAG AGGTCACACA 
CATCCACAGA CATCAACACC TCAGAGGCAT GATTTAAGGT CTTCAAAGAG GAGGAGAAGG 
CAGACAGAGA GAGCTGGCAA TGGAGAAAAA GAGAGAGGAA TTTCACCTAG ACCAGTCCTC 
AGACAAAGAT CAGCATCTTT ACCTCGACAT CGGCCTGAAA CTGATATGAC ATCTGATTCT 
GAGCATGCTC AATCTGAGCC AAGACCATCA ACGTCTAGAT ATGTCCCAGT GTCGCCTGTG 
AAATCAAGAG GAAGAAGACA AACCAGGAGA TTTGAATGTC ACAAAGAGGA TAGTACATCA 
GGCAGATCTG ACAACAGCGA TGAAGCAGAA CCAAACGGAG GAGCAGAATC TCTGTGTCCT 
GATTGCATAT CCGATCGTAC CAGTGCTTTG ACACGTCTCC TGAGGAGCAG GAGCTCTCCA 
CATCTTGCAC AGACTTCCTG TAGCCATTGC CCATTGTGTG GTGCATTACA AGCCATTCAA 
AGAGCCTCTA AGCCAGCTCC AGTCTCCAGT CCCAGAGCGA GTCAGCCAAT GAGATCCTTA 
CAGAGAGAAA AAGGAGGCGT GAACGTGACA GTGGCTCCTC CCCCTTTATT GCAGAGAGCT 
GTTCCAGTTG TCCCATATGT GCCTGTATAT CCTTCAACTC TATATTTCTC CAGCCCAATG 
ACTCCACCGG CATACCCACA ACCCCTCAAC ATCTCATCAA ATCCCGTCAG GCCTGAGAGG 
TCAGGGGTCA GAGGTCACCG CAGACGCTCT TCCTCCATGG AGTATCATCA GGATTCTATG 
AGCAGCGCTT TGACCCGTGC CATCACTGCA GCGAGACACA TGAGAGAAGC CTCACAGCGC 
ATGGCCAGCA GCCTCCACAC TGCATCCTGC AGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293531.1
CDS	489..4847
Translation	MERSRGGTSTGVLVWTPAPQLSSPSASEGSDFSWDEEPDQDFQSQMDENGIIGLAERQGQQEREEEEVAEELLWDVKTPEPEHGPPPAVEEISCHLSELLDSEPLSQATGDHYYSPSLQEEHQTDDDRSVLSEDWADDEGGTGSPVKHKLSSFPERDTALDMTDEETEEKNTQEDAQRFMDQSFHVTEERAAVFQTLKTLDFSQEMSGIFKDHPISGVPEYSDQSDCEELDARFLPVSHHFSRNTSNPTSFPHLSSEELMNDCGIEAETLPELEIRSDSSLGTYQSPVSKTNHTFRSNQEEIKHKISPQTSPRSPVIKNSPEKIYQDATSGEKLDGRLHRPYHNLQPPTVSPRKTTSASCNTQVSIPAQAISKASVSPSERSPPPTPHRNSAIKNHRNCKTSHMDPDDIRKGQLNHALPDFSKVEPKVRFPKRSEYKPPKSRRPPHVRTSYPGLPVVFKSPAEIVKEVLLSSCDGTTGSPSSSETQKHLHNIVPEEFRCPQQASTLMQQLQEDYNRLLTKYAEAENTIDRLRLEAKVGLSSEPPKPSSTAVLSGVIQEGSKVITISFPQAQRAEFSTGSVHLTQQKELSENSRKAATRPSSVSSISSRRSGSLTPDHLTDTLSKQNQRFQLQVDAFESLLKGGKLKPCEQTKGLSTLAEGQESLERAYLNARVQYQMLQQRQGRHAIFDPDRDLEGQIFRSGMRLEELKEWLEQAEQNQPVSEPTLSPYPPSDVLSASMLEPEPVPEIPLSAVCPEACVGVEVSSVSGESDSGREEMEMLPFVLHPLDNKHQRVEKDFSELMDCYQSVKELPGTLDQAVTLKSHDLLDFGSSVPPGKKSVTDGQPLKTVNEREMRKPAQQQPAAGCVPPSCPSVSSQVCDSMETHPEAFCHSYVLAGESTRESKTAGNMKAQSTSLTSLEERTHHRTVGLKAKAKTLRAPPMDGVMSPETDSGFMGSESSQLTAALHSPLHQRAVISSIRLSDPHTKDTERPESASPSGQPFIVSPSPSQPSPNTTKKHIDTSGKCTGPYKKIGGEERCSFSSLSSSLSPLHWPRSSILPWTSSLTSQSERGADEEEEKAQKDKYPQPTNQQLRSHRSPSLHRPYHHSSAQLTNHQKALQSLQKEVNRLKERLEGNLRLSKPARPMRTEVSASEDTRGHTHPQTSTPQRHDLRSSKRRRRQTERAGNGEKERGISPRPVLRQRSASLPRHRPETDMTSDSEHAQSEPRPSTSRYVPVSPVKSRGRRQTRRFECHKEDSTSGRSDNSDEAEPNGGAESLCPDCISDRTSALTRLLRSRSSPHLAQTSCSHCPLCGALQAIQRASKPAPVSSPRASQPMRSLQREKGGVNVTVAPPPLLQRAVPVVPYVPVYPSTLYFSSPMTPPAYPQPLNISSNPVRPERSGVRGHRRRSSSMEYHQDSMSSALTRAITAARHMREASQRMASSLHTASCSY

Target ORF information:

RefSeq Version	XM_009295256.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio AT-hook transcription factor (akna), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295256.2

ORF Insert Sequence:

ATGGAGCGGA GCAGAGGAGG CACCTCTACA GGGGTTCTGG TTTGGACACC TGCCCCACAG 
CTTTCCTCTC CCTCAGCCTC AGAGGGGTCA GACTTCAGCT GGGATGAAGA ACCGGACCAG 
GACTTCCAAA GCCAGATGGA TGAGAATGGG ATCATTGGTC TGGCAGAGAG GCAAGGGCAG 
CAGGAGAGAG AAGAGGAGGA GGTTGCAGAG GAGCTGTTGT GGGATGTGAA GACCCCTGAG 
CCTGAGCATG GGCCGCCGCC TGCAGTAGAG GAGATAAGCT GTCATCTGAG CGAACTGCTG 
GACTCTGAAC CGCTCTCTCA GGCCACTGGA GACCACTACT ACTCTCCATC ACTGCAGGAA 
GAACACCAAA CAGACGATGA TCGTAGTGTG TTGTCTGAGG ATTGGGCTGA TGACGAGGGT 
GGTACAGGGA GCCCAGTAAA GCACAAATTA AGCTCTTTCC CTGAGAGAGA CACTGCATTA 
GACATGACCG ATGAGGAGAC AGAAGAGAAA AACACACAAG AAGATGCTCA GAGATTCATG 
GATCAATCAT TTCATGTCAC AGAGGAGAGG GCAGCTGTGT TTCAAACTCT GAAAACCCTT 
GATTTCAGTC AGGAAATGAG TGGGATTTTC AAAGATCATC CAATTTCAGG AGTCCCAGAG 
TACTCTGATC AAAGTGACTG CGAGGAACTT GATGCCCGAT TTCTTCCAGT CTCGCATCAT 
TTCTCTAGAA ACACTTCCAA TCCCACCAGT TTTCCCCATT TATCCTCTGA GGAACTGATG 
AATGACTGTG GGATTGAAGC TGAGACGTTA CCTGAGCTAG AAATTAGGTC AGATTCATCT 
CTTGGCACTT ACCAGAGTCC TGTCTCTAAA ACAAACCACA CTTTCAGATC AAACCAGGAA 
GAGATTAAAC ACAAGATCTC ACCTCAAACT TCACCCAGAA GCCCTGTAAT AAAAAACAGC 
CCTGAAAAAA TATACCAAGA TGCTACGAGT GGCGAAAAAT TAGATGGAAG GTTACACAGG 
CCTTACCACA ATCTGCAGCC TCCAACAGTC TCTCCCAGAA AAACAACCTC TGCTTCCTGC 
AACACTCAGG TTTCCATTCC AGCGCAGGCT ATTTCTAAAG CAAGCGTCTC CCCCTCAGAG 
CGATCGCCTC CTCCTACACC TCACAGAAAT TCAGCAATAA AGAATCACAG AAACTGCAAA 
ACCTCTCACA TGGATCCAGA TGACATCAGG AAAGGACAGC TGAATCATGC CTTGCCTGAT 
TTCTCCAAAG TAGAGCCGAA GGTGCGTTTC CCAAAAAGAA GTGAATACAA ACCTCCCAAA 
AGCCGAAGAC CCCCTCATGT TCGAACATCA TACCCGGGTC TTCCAGTGGT GTTCAAATCT 
CCTGCTGAGA TTGTGAAAGA GGTCCTGTTG AGCAGCTGTG ATGGGACTAC TGGGAGTCCA 
TCCTCCAGCG AGACTCAGAA GCACCTGCAC AATATCGTCC CAGAAGAGTT TCGCTGCCCA 
CAGCAGGCCA GCACACTGAT GCAGCAGCTA CAGGAGGACT ATAACAGGCT TCTTACCAAG 
TATGCAGAAG CTGAAAATAC TATTGATCGA CTTCGCCTTG AAGCAAAGGT GGGTCTCTCT 
TCGGAACCAC CCAAACCCAG TAGTACAGCT GTCCTGTCGG GAGTTATTCA GGAAGGGTCA 
AAGGTCATAA CCATCAGTTT TCCTCAAGCA CAGAGGGCAG AGTTCAGTAC AGGTTCTGTT 
CATCTGACCC AACAGAAGGA GCTCTCAGAA AACTCCAGAA AAGCAGCAAC TCGGCCTTCT 
TCTGTAAGCT CTATCAGCTC GAGGAGGTCT GGATCTCTTA CTCCAGACCA TCTGACAGAC 
ACTCTGTCCA AACAGAACCA AAGATTTCAG CTACAGGTGG ATGCATTTGA GTCGCTTTTA 
AAGGGTGGGA AGCTCAAGCC ATGTGAGCAG ACAAAGGGTC TCTCCACACT GGCTGAGGGG 
CAGGAGTCTC TTGAGAGGGC TTATCTAAAT GCACGAGTCC AATATCAGAT GCTGCAGCAA 
CGACAAGGCA GACATGCCAT CTTTGACCCA GACAGGGATC TAGAGGGTCA GATCTTCAGG 
TCAGGGATGC GATTAGAGGA GCTGAAAGAG TGGCTGGAAC AAGCAGAACA GAACCAACCT 
GTTTCTGAAC CTACTTTATC CCCATATCCT CCTTCAGATG TCCTTTCTGC ATCCATGCTG 
GAGCCTGAAC CAGTGCCTGA GATCCCCCTG TCAGCAGTCT GTCCTGAGGC TTGTGTTGGT 
GTGGAGGTCA GTTCAGTCAG TGGAGAGAGT GATAGTGGTA GAGAGGAAAT GGAAATGCTT 
CCGTTTGTTC TTCATCCCCT CGATAACAAA CACCAACGTG TGGAGAAAGA TTTCAGTGAA 
CTCATGGACT GCTATCAGAG TGTTAAAGAG CTCCCCGGGA CACTGGATCA GGCTGTGACT 
TTAAAGAGCC ATGACTTGCT GGATTTTGGA AGCTCTGTTC CTCCTGGGAA AAAAAGTGTA 
ACGGACGGAC AACCACTCAA GACTGTTAAT GAGAGAGAGA TGAGAAAACC AGCTCAGCAG 
CAGCCGGCAG CAGGTTGTGT CCCTCCCTCA TGCCCATCTG TCAGTTCACA GGTGTGCGAC 
TCTATGGAAA CTCACCCAGA GGCTTTTTGC CACTCCTATG TATTGGCAGG AGAATCGACC 
AGAGAGAGTA AAACAGCAGG AAACATGAAA GCTCAAAGCA CTAGCTTGAC CAGCCTGGAA 
GAGAGAACAC ACCACAGAAC TGTAGGCCTG AAAGCGAAGG CGAAGACTCT AAGAGCGCCC 
CCTATGGATG GGGTCATGTC TCCAGAAACA GACAGTGGCT TTATGGGCTC TGAAAGCAGT 
CAGCTCACTG CTGCATTACA CAGTCCTCTC CACCAGAGGG CAGTAATAAG TTCCATCAGA 
CTGTCTGACC CTCACACAAA GGACACAGAG AGACCAGAGT CTGCTTCTCC TTCTGGGCAG 
CCTTTCATTG TCTCTCCTTC TCCATCCCAA CCTTCTCCAA ACACCACAAA AAAGCACATT 
GATACATCAG GAAAATGCAC AGGTCCTTAT AAGAAAATAG GAGGGGAGGA AAGGTGTTCT 
TTTTCCAGTC TTTCTTCCTC CTTATCCCCC CTTCACTGGC CACGTAGCAG CATTCTGCCC 
TGGACAAGCA GCCTGACCAG TCAATCAGAG CGTGGAGCTG ATGAGGAAGA AGAAAAAGCA 
CAGAAAGACA AATACCCACA ACCGACCAAT CAGCAACTCC GCTCTCACAG AAGCCCCTCC 
CTCCACAGGC CTTATCACCA CAGTTCTGCT CAGCTAACAA ATCACCAAAA GGCTCTTCAA 
TCTCTGCAAA AAGAAGTGAA CCGATTGAAA GAGAGATTAG AGGGAAATCT GAGACTATCA 
AAACCTGCCA GACCTATGAG AACAGAAGTT TCTGCCTCTG AAGACACCAG AGGTCACACA 
CATCCACAGA CATCAACACC TCAGAGGCAT GATTTAAGGT CTTCAAAGAG GAGGAGAAGG 
CAGACAGAGA GAGCTGGCAA TGGAGAAAAA GAGAGAGGAA TTTCACCTAG ACCAGTCCTC 
AGACAAAGAT CAGCATCTTT ACCTCGACAT CGGCCTGAAA CTGATATGAC ATCTGATTCT 
GAGCATGCTC AATCTGAGCC AAGACCATCA ACGTCTAGAT ATGTCCCAGT GTCGCCTGTG 
AAATCAAGAG GAAGAAGACA AACCAGGAGA TTTGAATGTC ACAAAGAGGA TAGTACATCA 
GGCAGATCTG ACAACAGCGA TGAAGCAGAA CCAAACGGAG GAGCAGAATC TCTGTGTCCT 
GATTGCATAT CCGATCGTAC CAGTGCTTTG ACACGTCTCC TGAGGAGCAG GAGCTCTCCA 
CATCTTGCAC AGACTTCCTG TAGCCATTGC CCATTGTGTG GTGCATTACA AGCCATTCAA 
AGAGCCTCTA AGCCAGCTCC AGTCTCCAGT CCCAGAGCGA GTCAGCCAAT GAGATCCTTA 
CAGAGAGAAA AAGGAGGCGT GAACGTGACA GTGGCTCCTC CCCCTTTATT GCAGAGAGCT 
GTTCCAGTTG TCCCATATGT GCCTGTATAT CCTTCAACTC TATATTTCTC CAGCCCAATG 
ACTCCACCGG CATACCCACA ACCCCTCAAC ATCTCATCAA ATCCCGTCAG GCCTGAGAGG 
TCAGGGGTCA GAGGTCACCG CAGACGCTCT TCCTCCATGG AGTATCATCA GGATTCTATG 
AGCAGCGCTT TGACCCGTGC CATCACTGCA GCGAGACACA TGAGAGAAGC CTCACAGCGC 
ATGGCCAGCA GCCTCCACAC TGCATCCTGC AGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

akna ( XM_009295257.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293532.1 Danio rerio AT-hook transcription factor (akna), transcript variant X4, mRNA.

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CloneID	ODa25697
Clone ID Related Accession (Same CDS sequence)	XM_009295258.3 , XM_009295258.2 , XM_009295256.3 , XM_005161116.4 , XM_678843.6 , XM_005161116.3 , XM_009295257.2 , XM_678843.7 , XM_017353013.1 , XM_009295256.2 , XM_009295257.3
Accession Version	XM_009295257.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	AT-hook-containing transcription factor-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009295257.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGGA GCAGAGGAGG CACCTCTACA GGGGTTCTGG TTTGGACACC TGCCCCACAG 
CTTTCCTCTC CCTCAGCCTC AGAGGGGTCA GACTTCAGCT GGGATGAAGA ACCGGACCAG 
GACTTCCAAA GCCAGATGGA TGAGAATGGG ATCATTGGTC TGGCAGAGAG GCAAGGGCAG 
CAGGAGAGAG AAGAGGAGGA GGTTGCAGAG GAGCTGTTGT GGGATGTGAA GACCCCTGAG 
CCTGAGCATG GGCCGCCGCC TGCAGTAGAG GAGATAAGCT GTCATCTGAG CGAACTGCTG 
GACTCTGAAC CGCTCTCTCA GGCCACTGGA GACCACTACT ACTCTCCATC ACTGCAGGAA 
GAACACCAAA CAGACGATGA TCGTAGTGTG TTGTCTGAGG ATTGGGCTGA TGACGAGGGT 
GGTACAGGGA GCCCAGTAAA GCACAAATTA AGCTCTTTCC CTGAGAGAGA CACTGCATTA 
GACATGACCG ATGAGGAGAC AGAAGAGAAA AACACACAAG AAGATGCTCA GAGATTCATG 
GATCAATCAT TTCATGTCAC AGAGGAGAGG GCAGCTGTGT TTCAAACTCT GAAAACCCTT 
GATTTCAGTC AGGAAATGAG TGGGATTTTC AAAGATCATC CAATTTCAGG AGTCCCAGAG 
TACTCTGATC AAAGTGACTG CGAGGAACTT GATGCCCGAT TTCTTCCAGT CTCGCATCAT 
TTCTCTAGAA ACACTTCCAA TCCCACCAGT TTTCCCCATT TATCCTCTGA GGAACTGATG 
AATGACTGTG GGATTGAAGC TGAGACGTTA CCTGAGCTAG AAATTAGGTC AGATTCATCT 
CTTGGCACTT ACCAGAGTCC TGTCTCTAAA ACAAACCACA CTTTCAGATC AAACCAGGAA 
GAGATTAAAC ACAAGATCTC ACCTCAAACT TCACCCAGAA GCCCTGTAAT AAAAAACAGC 
CCTGAAAAAA TATACCAAGA TGCTACGAGT GGCGAAAAAT TAGATGGAAG GTTACACAGG 
CCTTACCACA ATCTGCAGCC TCCAACAGTC TCTCCCAGAA AAACAACCTC TGCTTCCTGC 
AACACTCAGG TTTCCATTCC AGCGCAGGCT ATTTCTAAAG CAAGCGTCTC CCCCTCAGAG 
CGATCGCCTC CTCCTACACC TCACAGAAAT TCAGCAATAA AGAATCACAG AAACTGCAAA 
ACCTCTCACA TGGATCCAGA TGACATCAGG AAAGGACAGC TGAATCATGC CTTGCCTGAT 
TTCTCCAAAG TAGAGCCGAA GGTGCGTTTC CCAAAAAGAA GTGAATACAA ACCTCCCAAA 
AGCCGAAGAC CCCCTCATGT TCGAACATCA TACCCGGGTC TTCCAGTGGT GTTCAAATCT 
CCTGCTGAGA TTGTGAAAGA GGTCCTGTTG AGCAGCTGTG ATGGGACTAC TGGGAGTCCA 
TCCTCCAGCG AGACTCAGAA GCACCTGCAC AATATCGTCC CAGAAGAGTT TCGCTGCCCA 
CAGCAGGCCA GCACACTGAT GCAGCAGCTA CAGGAGGACT ATAACAGGCT TCTTACCAAG 
TATGCAGAAG CTGAAAATAC TATTGATCGA CTTCGCCTTG AAGCAAAGGT GGGTCTCTCT 
TCGGAACCAC CCAAACCCAG TAGTACAGCT GTCCTGTCGG GAGTTATTCA GGAAGGGTCA 
AAGGTCATAA CCATCAGTTT TCCTCAAGCA CAGAGGGCAG AGTTCAGTAC AGGTTCTGTT 
CATCTGACCC AACAGAAGGA GCTCTCAGAA AACTCCAGAA AAGCAGCAAC TCGGCCTTCT 
TCTGTAAGCT CTATCAGCTC GAGGAGGTCT GGATCTCTTA CTCCAGACCA TCTGACAGAC 
ACTCTGTCCA AACAGAACCA AAGATTTCAG CTACAGGTGG ATGCATTTGA GTCGCTTTTA 
AAGGGTGGGA AGCTCAAGCC ATGTGAGCAG ACAAAGGGTC TCTCCACACT GGCTGAGGGG 
CAGGAGTCTC TTGAGAGGGC TTATCTAAAT GCACGAGTCC AATATCAGAT GCTGCAGCAA 
CGACAAGGCA GACATGCCAT CTTTGACCCA GACAGGGATC TAGAGGGTCA GATCTTCAGG 
TCAGGGATGC GATTAGAGGA GCTGAAAGAG TGGCTGGAAC AAGCAGAACA GAACCAACCT 
GTTTCTGAAC CTACTTTATC CCCATATCCT CCTTCAGATG TCCTTTCTGC ATCCATGCTG 
GAGCCTGAAC CAGTGCCTGA GATCCCCCTG TCAGCAGTCT GTCCTGAGGC TTGTGTTGGT 
GTGGAGGTCA GTTCAGTCAG TGGAGAGAGT GATAGTGGTA GAGAGGAAAT GGAAATGCTT 
CCGTTTGTTC TTCATCCCCT CGATAACAAA CACCAACGTG TGGAGAAAGA TTTCAGTGAA 
CTCATGGACT GCTATCAGAG TGTTAAAGAG CTCCCCGGGA CACTGGATCA GGCTGTGACT 
TTAAAGAGCC ATGACTTGCT GGATTTTGGA AGCTCTGTTC CTCCTGGGAA AAAAAGTGTA 
ACGGACGGAC AACCACTCAA GACTGTTAAT GAGAGAGAGA TGAGAAAACC AGCTCAGCAG 
CAGCCGGCAG CAGGTTGTGT CCCTCCCTCA TGCCCATCTG TCAGTTCACA GGTGTGCGAC 
TCTATGGAAA CTCACCCAGA GGCTTTTTGC CACTCCTATG TATTGGCAGG AGAATCGACC 
AGAGAGAGTA AAACAGCAGG AAACATGAAA GCTCAAAGCA CTAGCTTGAC CAGCCTGGAA 
GAGAGAACAC ACCACAGAAC TGTAGGCCTG AAAGCGAAGG CGAAGACTCT AAGAGCGCCC 
CCTATGGATG GGGTCATGTC TCCAGAAACA GACAGTGGCT TTATGGGCTC TGAAAGCAGT 
CAGCTCACTG CTGCATTACA CAGTCCTCTC CACCAGAGGG CAGTAATAAG TTCCATCAGA 
CTGTCTGACC CTCACACAAA GGACACAGAG AGACCAGAGT CTGCTTCTCC TTCTGGGCAG 
CCTTTCATTG TCTCTCCTTC TCCATCCCAA CCTTCTCCAA ACACCACAAA AAAGCACATT 
GATACATCAG GAAAATGCAC AGGTCCTTAT AAGAAAATAG GAGGGGAGGA AAGGTGTTCT 
TTTTCCAGTC TTTCTTCCTC CTTATCCCCC CTTCACTGGC CACGTAGCAG CATTCTGCCC 
TGGACAAGCA GCCTGACCAG TCAATCAGAG CGTGGAGCTG ATGAGGAAGA AGAAAAAGCA 
CAGAAAGACA AATACCCACA ACCGACCAAT CAGCAACTCC GCTCTCACAG AAGCCCCTCC 
CTCCACAGGC CTTATCACCA CAGTTCTGCT CAGCTAACAA ATCACCAAAA GGCTCTTCAA 
TCTCTGCAAA AAGAAGTGAA CCGATTGAAA GAGAGATTAG AGGGAAATCT GAGACTATCA 
AAACCTGCCA GACCTATGAG AACAGAAGTT TCTGCCTCTG AAGACACCAG AGGTCACACA 
CATCCACAGA CATCAACACC TCAGAGGCAT GATTTAAGGT CTTCAAAGAG GAGGAGAAGG 
CAGACAGAGA GAGCTGGCAA TGGAGAAAAA GAGAGAGGAA TTTCACCTAG ACCAGTCCTC 
AGACAAAGAT CAGCATCTTT ACCTCGACAT CGGCCTGAAA CTGATATGAC ATCTGATTCT 
GAGCATGCTC AATCTGAGCC AAGACCATCA ACGTCTAGAT ATGTCCCAGT GTCGCCTGTG 
AAATCAAGAG GAAGAAGACA AACCAGGAGA TTTGAATGTC ACAAAGAGGA TAGTACATCA 
GGCAGATCTG ACAACAGCGA TGAAGCAGAA CCAAACGGAG GAGCAGAATC TCTGTGTCCT 
GATTGCATAT CCGATCGTAC CAGTGCTTTG ACACGTCTCC TGAGGAGCAG GAGCTCTCCA 
CATCTTGCAC AGACTTCCTG TAGCCATTGC CCATTGTGTG GTGCATTACA AGCCATTCAA 
AGAGCCTCTA AGCCAGCTCC AGTCTCCAGT CCCAGAGCGA GTCAGCCAAT GAGATCCTTA 
CAGAGAGAAA AAGGAGGCGT GAACGTGACA GTGGCTCCTC CCCCTTTATT GCAGAGAGCT 
GTTCCAGTTG TCCCATATGT GCCTGTATAT CCTTCAACTC TATATTTCTC CAGCCCAATG 
ACTCCACCGG CATACCCACA ACCCCTCAAC ATCTCATCAA ATCCCGTCAG GCCTGAGAGG 
TCAGGGGTCA GAGGTCACCG CAGACGCTCT TCCTCCATGG AGTATCATCA GGATTCTATG 
AGCAGCGCTT TGACCCGTGC CATCACTGCA GCGAGACACA TGAGAGAAGC CTCACAGCGC 
ATGGCCAGCA GCCTCCACAC TGCATCCTGC AGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293532.1
CDS	258..4616
Translation	MERSRGGTSTGVLVWTPAPQLSSPSASEGSDFSWDEEPDQDFQSQMDENGIIGLAERQGQQEREEEEVAEELLWDVKTPEPEHGPPPAVEEISCHLSELLDSEPLSQATGDHYYSPSLQEEHQTDDDRSVLSEDWADDEGGTGSPVKHKLSSFPERDTALDMTDEETEEKNTQEDAQRFMDQSFHVTEERAAVFQTLKTLDFSQEMSGIFKDHPISGVPEYSDQSDCEELDARFLPVSHHFSRNTSNPTSFPHLSSEELMNDCGIEAETLPELEIRSDSSLGTYQSPVSKTNHTFRSNQEEIKHKISPQTSPRSPVIKNSPEKIYQDATSGEKLDGRLHRPYHNLQPPTVSPRKTTSASCNTQVSIPAQAISKASVSPSERSPPPTPHRNSAIKNHRNCKTSHMDPDDIRKGQLNHALPDFSKVEPKVRFPKRSEYKPPKSRRPPHVRTSYPGLPVVFKSPAEIVKEVLLSSCDGTTGSPSSSETQKHLHNIVPEEFRCPQQASTLMQQLQEDYNRLLTKYAEAENTIDRLRLEAKVGLSSEPPKPSSTAVLSGVIQEGSKVITISFPQAQRAEFSTGSVHLTQQKELSENSRKAATRPSSVSSISSRRSGSLTPDHLTDTLSKQNQRFQLQVDAFESLLKGGKLKPCEQTKGLSTLAEGQESLERAYLNARVQYQMLQQRQGRHAIFDPDRDLEGQIFRSGMRLEELKEWLEQAEQNQPVSEPTLSPYPPSDVLSASMLEPEPVPEIPLSAVCPEACVGVEVSSVSGESDSGREEMEMLPFVLHPLDNKHQRVEKDFSELMDCYQSVKELPGTLDQAVTLKSHDLLDFGSSVPPGKKSVTDGQPLKTVNEREMRKPAQQQPAAGCVPPSCPSVSSQVCDSMETHPEAFCHSYVLAGESTRESKTAGNMKAQSTSLTSLEERTHHRTVGLKAKAKTLRAPPMDGVMSPETDSGFMGSESSQLTAALHSPLHQRAVISSIRLSDPHTKDTERPESASPSGQPFIVSPSPSQPSPNTTKKHIDTSGKCTGPYKKIGGEERCSFSSLSSSLSPLHWPRSSILPWTSSLTSQSERGADEEEEKAQKDKYPQPTNQQLRSHRSPSLHRPYHHSSAQLTNHQKALQSLQKEVNRLKERLEGNLRLSKPARPMRTEVSASEDTRGHTHPQTSTPQRHDLRSSKRRRRQTERAGNGEKERGISPRPVLRQRSASLPRHRPETDMTSDSEHAQSEPRPSTSRYVPVSPVKSRGRRQTRRFECHKEDSTSGRSDNSDEAEPNGGAESLCPDCISDRTSALTRLLRSRSSPHLAQTSCSHCPLCGALQAIQRASKPAPVSSPRASQPMRSLQREKGGVNVTVAPPPLLQRAVPVVPYVPVYPSTLYFSSPMTPPAYPQPLNISSNPVRPERSGVRGHRRRSSSMEYHQDSMSSALTRAITAARHMREASQRMASSLHTASCSY

Target ORF information:

RefSeq Version	XM_009295257.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio AT-hook transcription factor (akna), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295257.3

ORF Insert Sequence:

ATGGAGCGGA GCAGAGGAGG CACCTCTACA GGGGTTCTGG TTTGGACACC TGCCCCACAG 
CTTTCCTCTC CCTCAGCCTC AGAGGGGTCA GACTTCAGCT GGGATGAAGA ACCGGACCAG 
GACTTCCAAA GCCAGATGGA TGAGAATGGG ATCATTGGTC TGGCAGAGAG GCAAGGGCAG 
CAGGAGAGAG AAGAGGAGGA GGTTGCAGAG GAGCTGTTGT GGGATGTGAA GACCCCTGAG 
CCTGAGCATG GGCCGCCGCC TGCAGTAGAG GAGATAAGCT GTCATCTGAG CGAACTGCTG 
GACTCTGAAC CGCTCTCTCA GGCCACTGGA GACCACTACT ACTCTCCATC ACTGCAGGAA 
GAACACCAAA CAGACGATGA TCGTAGTGTG TTGTCTGAGG ATTGGGCTGA TGACGAGGGT 
GGTACAGGGA GCCCAGTAAA GCACAAATTA AGCTCTTTCC CTGAGAGAGA CACTGCATTA 
GACATGACCG ATGAGGAGAC AGAAGAGAAA AACACACAAG AAGATGCTCA GAGATTCATG 
GATCAATCAT TTCATGTCAC AGAGGAGAGG GCAGCTGTGT TTCAAACTCT GAAAACCCTT 
GATTTCAGTC AGGAAATGAG TGGGATTTTC AAAGATCATC CAATTTCAGG AGTCCCAGAG 
TACTCTGATC AAAGTGACTG CGAGGAACTT GATGCCCGAT TTCTTCCAGT CTCGCATCAT 
TTCTCTAGAA ACACTTCCAA TCCCACCAGT TTTCCCCATT TATCCTCTGA GGAACTGATG 
AATGACTGTG GGATTGAAGC TGAGACGTTA CCTGAGCTAG AAATTAGGTC AGATTCATCT 
CTTGGCACTT ACCAGAGTCC TGTCTCTAAA ACAAACCACA CTTTCAGATC AAACCAGGAA 
GAGATTAAAC ACAAGATCTC ACCTCAAACT TCACCCAGAA GCCCTGTAAT AAAAAACAGC 
CCTGAAAAAA TATACCAAGA TGCTACGAGT GGCGAAAAAT TAGATGGAAG GTTACACAGG 
CCTTACCACA ATCTGCAGCC TCCAACAGTC TCTCCCAGAA AAACAACCTC TGCTTCCTGC 
AACACTCAGG TTTCCATTCC AGCGCAGGCT ATTTCTAAAG CAAGCGTCTC CCCCTCAGAG 
CGATCGCCTC CTCCTACACC TCACAGAAAT TCAGCAATAA AGAATCACAG AAACTGCAAA 
ACCTCTCACA TGGATCCAGA TGACATCAGG AAAGGACAGC TGAATCATGC CTTGCCTGAT 
TTCTCCAAAG TAGAGCCGAA GGTGCGTTTC CCAAAAAGAA GTGAATACAA ACCTCCCAAA 
AGCCGAAGAC CCCCTCATGT TCGAACATCA TACCCGGGTC TTCCAGTGGT GTTCAAATCT 
CCTGCTGAGA TTGTGAAAGA GGTCCTGTTG AGCAGCTGTG ATGGGACTAC TGGGAGTCCA 
TCCTCCAGCG AGACTCAGAA GCACCTGCAC AATATCGTCC CAGAAGAGTT TCGCTGCCCA 
CAGCAGGCCA GCACACTGAT GCAGCAGCTA CAGGAGGACT ATAACAGGCT TCTTACCAAG 
TATGCAGAAG CTGAAAATAC TATTGATCGA CTTCGCCTTG AAGCAAAGGT GGGTCTCTCT 
TCGGAACCAC CCAAACCCAG TAGTACAGCT GTCCTGTCGG GAGTTATTCA GGAAGGGTCA 
AAGGTCATAA CCATCAGTTT TCCTCAAGCA CAGAGGGCAG AGTTCAGTAC AGGTTCTGTT 
CATCTGACCC AACAGAAGGA GCTCTCAGAA AACTCCAGAA AAGCAGCAAC TCGGCCTTCT 
TCTGTAAGCT CTATCAGCTC GAGGAGGTCT GGATCTCTTA CTCCAGACCA TCTGACAGAC 
ACTCTGTCCA AACAGAACCA AAGATTTCAG CTACAGGTGG ATGCATTTGA GTCGCTTTTA 
AAGGGTGGGA AGCTCAAGCC ATGTGAGCAG ACAAAGGGTC TCTCCACACT GGCTGAGGGG 
CAGGAGTCTC TTGAGAGGGC TTATCTAAAT GCACGAGTCC AATATCAGAT GCTGCAGCAA 
CGACAAGGCA GACATGCCAT CTTTGACCCA GACAGGGATC TAGAGGGTCA GATCTTCAGG 
TCAGGGATGC GATTAGAGGA GCTGAAAGAG TGGCTGGAAC AAGCAGAACA GAACCAACCT 
GTTTCTGAAC CTACTTTATC CCCATATCCT CCTTCAGATG TCCTTTCTGC ATCCATGCTG 
GAGCCTGAAC CAGTGCCTGA GATCCCCCTG TCAGCAGTCT GTCCTGAGGC TTGTGTTGGT 
GTGGAGGTCA GTTCAGTCAG TGGAGAGAGT GATAGTGGTA GAGAGGAAAT GGAAATGCTT 
CCGTTTGTTC TTCATCCCCT CGATAACAAA CACCAACGTG TGGAGAAAGA TTTCAGTGAA 
CTCATGGACT GCTATCAGAG TGTTAAAGAG CTCCCCGGGA CACTGGATCA GGCTGTGACT 
TTAAAGAGCC ATGACTTGCT GGATTTTGGA AGCTCTGTTC CTCCTGGGAA AAAAAGTGTA 
ACGGACGGAC AACCACTCAA GACTGTTAAT GAGAGAGAGA TGAGAAAACC AGCTCAGCAG 
CAGCCGGCAG CAGGTTGTGT CCCTCCCTCA TGCCCATCTG TCAGTTCACA GGTGTGCGAC 
TCTATGGAAA CTCACCCAGA GGCTTTTTGC CACTCCTATG TATTGGCAGG AGAATCGACC 
AGAGAGAGTA AAACAGCAGG AAACATGAAA GCTCAAAGCA CTAGCTTGAC CAGCCTGGAA 
GAGAGAACAC ACCACAGAAC TGTAGGCCTG AAAGCGAAGG CGAAGACTCT AAGAGCGCCC 
CCTATGGATG GGGTCATGTC TCCAGAAACA GACAGTGGCT TTATGGGCTC TGAAAGCAGT 
CAGCTCACTG CTGCATTACA CAGTCCTCTC CACCAGAGGG CAGTAATAAG TTCCATCAGA 
CTGTCTGACC CTCACACAAA GGACACAGAG AGACCAGAGT CTGCTTCTCC TTCTGGGCAG 
CCTTTCATTG TCTCTCCTTC TCCATCCCAA CCTTCTCCAA ACACCACAAA AAAGCACATT 
GATACATCAG GAAAATGCAC AGGTCCTTAT AAGAAAATAG GAGGGGAGGA AAGGTGTTCT 
TTTTCCAGTC TTTCTTCCTC CTTATCCCCC CTTCACTGGC CACGTAGCAG CATTCTGCCC 
TGGACAAGCA GCCTGACCAG TCAATCAGAG CGTGGAGCTG ATGAGGAAGA AGAAAAAGCA 
CAGAAAGACA AATACCCACA ACCGACCAAT CAGCAACTCC GCTCTCACAG AAGCCCCTCC 
CTCCACAGGC CTTATCACCA CAGTTCTGCT CAGCTAACAA ATCACCAAAA GGCTCTTCAA 
TCTCTGCAAA AAGAAGTGAA CCGATTGAAA GAGAGATTAG AGGGAAATCT GAGACTATCA 
AAACCTGCCA GACCTATGAG AACAGAAGTT TCTGCCTCTG AAGACACCAG AGGTCACACA 
CATCCACAGA CATCAACACC TCAGAGGCAT GATTTAAGGT CTTCAAAGAG GAGGAGAAGG 
CAGACAGAGA GAGCTGGCAA TGGAGAAAAA GAGAGAGGAA TTTCACCTAG ACCAGTCCTC 
AGACAAAGAT CAGCATCTTT ACCTCGACAT CGGCCTGAAA CTGATATGAC ATCTGATTCT 
GAGCATGCTC AATCTGAGCC AAGACCATCA ACGTCTAGAT ATGTCCCAGT GTCGCCTGTG 
AAATCAAGAG GAAGAAGACA AACCAGGAGA TTTGAATGTC ACAAAGAGGA TAGTACATCA 
GGCAGATCTG ACAACAGCGA TGAAGCAGAA CCAAACGGAG GAGCAGAATC TCTGTGTCCT 
GATTGCATAT CCGATCGTAC CAGTGCTTTG ACACGTCTCC TGAGGAGCAG GAGCTCTCCA 
CATCTTGCAC AGACTTCCTG TAGCCATTGC CCATTGTGTG GTGCATTACA AGCCATTCAA 
AGAGCCTCTA AGCCAGCTCC AGTCTCCAGT CCCAGAGCGA GTCAGCCAAT GAGATCCTTA 
CAGAGAGAAA AAGGAGGCGT GAACGTGACA GTGGCTCCTC CCCCTTTATT GCAGAGAGCT 
GTTCCAGTTG TCCCATATGT GCCTGTATAT CCTTCAACTC TATATTTCTC CAGCCCAATG 
ACTCCACCGG CATACCCACA ACCCCTCAAC ATCTCATCAA ATCCCGTCAG GCCTGAGAGG 
TCAGGGGTCA GAGGTCACCG CAGACGCTCT TCCTCCATGG AGTATCATCA GGATTCTATG 
AGCAGCGCTT TGACCCGTGC CATCACTGCA GCGAGACACA TGAGAGAAGC CTCACAGCGC 
ATGGCCAGCA GCCTCCACAC TGCATCCTGC AGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

akna ( XM_009295259.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293534.1 Danio rerio AT-hook transcription factor (akna), transcript variant X6, mRNA.

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CloneID	ODa42303
Clone ID Related Accession (Same CDS sequence)	XM_009295259.3 , XM_009295259.2
Accession Version	XM_009295259.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	AT-hook-containing transcription factor-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009295259.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGGA GCAGAGGAGG CACCTCTACA GGGGTTCTGG TTTGGACACC TGCCCCACAG 
CTTTCCTCTC CCTCAGCCTC AGAGGGGTCA GACTTCAGCT GGGATGAAGA ACCGGACCAG 
GACTTCCAAA GCCAGATGGA TGAGAATGGG ATCATTGGTC TGGCAGAGAG GCAAGGGCAG 
CAGGAGAGAG AAGAGGAGGA GGTTGCAGAG GAGCTGTTGT GGGATGTGAA GACCCCTGAG 
CCTGAGCATG GGCCGCCGCC TGCAGTAGAG GAGATAAGCT GTCATCTGAG CGAACTGCTG 
GACTCTGAAC CGCTCTCTCA GGCCACTGGA GACCACTACT ACTCTCCATC ACTGCAGGAA 
GAACACCAAA CAGACGATGA TCGTAGTGTG TTGTCTGAGG ATTGGGCTGA TGACGAGGGT 
GGTACAGGGA GCCCAGTAAA GCACAAATTA AGCTCTTTCC CTGAGAGAGA CACTGCATTA 
GACATGACCG ATGAGGAGAC AGAAGAGAAA AACACACAAG AAGATGCTCA GAGATTCATG 
GATCAATCAT TTCATGTCAC AGAGGAGAGG GCAGCTGTGT TTCAAACTCT GAAAACCCTT 
GATTTCAGTC AGGAAATGAG TGGGATTTTC AAAGATCATC CAATTTCAGG AGTCCCAGAG 
TACTCTGATC AAAGTGACTG CGAGGAACTT GATGCCCGAT TTCTTCCAGT CTCGCATCAT 
TTCTCTAGAA ACACTTCCAA TCCCACCAGT TTTCCCCATT TATCCTCTGA GGAACTGATG 
AATGACTGTG GGATTGAAGC TGAGACGTTA CCTGAGCTAG AAATTAGGTC AGATTCATCT 
CTTGGCACTT ACCAGAGTCC TGTCTCTAAA ACAAACCACA CTTTCAGATC AAACCAGGAA 
GAGATTAAAC ACAAGATCTC ACCTCAAACT TCACCCAGAA GCCCTGTAAT AAAAAACAGC 
CCTGAAAAAA TATACCAAGA TGCTACGAGT GGCGAAAAAT TAGATGGAAG GTTACACAGG 
CCTTACCACA ATCTGCAGCC TCCAACAGTC TCTCCCAGAA AAACAACCTC TGCTTCCTGC 
AACACTCAGG TTTCCATTCC AGCGCAGGCT ATTTCTAAAG CAAGCGTCTC CCCCTCAGAG 
CGATCGCCTC CTCCTACACC TCACAGAAAT TCAGCAATAA AGAATCACAG AAACTGCAAA 
ACCTCTCACA TGGATCCAGA TGACATCAGG AAAGGACAGC TGAATCATGC CTTGCCTGAT 
TTCTCCAAAG TAGAGCCGAA GGTGCGTTTC CCAAAAAGAA GTGAATACAA ACCTCCCAAA 
AGCCGAAGAC CCCCTCATGT TCGAACATCA TACCCGGGTC TTCCAGTGGT GTTCAAATCT 
CCTGCTGAGA TTGTGAAAGA GGTCCTGTTG AGCAGCTGTG ATGGGACTAC TGGGAGTCCA 
TCCTCCAGCG AGACTCAGAA GCACCTGCAC AATATCGTCC CAGAAGAGTT TCGCTGCCCA 
CAGCAGGCCA GCACACTGAT GCAGCAGCTA CAGGAGGACT ATAACAGGCT TCTTACCAAG 
TATGCAGAAG CTGAAAATAC TATTGATCGA CTTCGCCTTG AAGCAAAGGT GGGTCTCTCT 
TCGGAACCAC CCAAACCCAG TAGTACAGCT GTCCTGTCGG GAGTTATTCA GGAAGGGTCA 
AAGGTCATAA CCATCAGTTT TCCTCAAGCA CAGAGGGCAG AGTTCAGTAC AGGTTCTGTT 
CATCTGACCC AACAGAAGGA GCTCTCAGAA AACTCCAGAA AAGCAGCAAC TCGGCCTTCT 
TCTGTAAGCT CTATCAGCTC GAGGAGGTCT GGATCTCTTA CTCCAGACCA TCTGACAGAC 
ACTCTGTCCA AACAGAACCA AAGATTTCAG CTACAGGTGG ATGCATTTGA GTCGCTTTTA 
AAGGGTGGGA AGCTCAAGCC ATGTGAGCAG ACAAAGGGTC TCTCCACACT GGCTGAGGGG 
CAGGAGTCTC TTGAGAGGGC TTATCTAAAT GCACGAGTCC AATATCAGAT GCTGCAGCAA 
CGACAAGGCA GACATGCCAT CTTTGACCCA GACAGGGATC TAGAGGGTCA GATCTTCAGG 
TCAGGGATGC GATTAGAGGA GCTGAAAGAG TGGCTGGAAC AAGCAGAACA GAACCAACCT 
GTTTCTGAAC CTACTTTATC CCCATATCCT CCTTCAGATG TCCTTTCTGC ATCCATGCTG 
GAGCCTGAAC CAGTGCCTGA GATCCCCCTG TCAGCAGTCT GTCCTGAGGC TTGTGTTGGT 
GTGGAGGTCA GTTCAGTCAG TGGAGAGAGT GATAGTGGTA GAGAGGAAAT GGAAATGCTT 
CCGTTTGTTC TTCATCCCCT CGATAACAAA CACCAACGTG TGGAGAAAGA TTTCAGTGAA 
CTCATGGACT GCTATCAGAG TGTTAAAGAG CTCCCCGGGA CACTGGATCA GGCTGTGACT 
TTAAAGAGCC ATGACTTGCT GGATTTTGGA AGCTCTGTTC CTCCTGGGAA AAAAAGTGTA 
ACGGACGGAC AACCACTCAA GACTGTTAAT GAGAGAGAGA TGAGAAAACC AGCTCAGCAG 
CAGCCGGCAG CAGGTTGTGT CCCTCCCTCA TGCCCATCTG TCAGTTCACA GGTGTGCGAC 
TCTATGGAAA CTCACCCAGA GGCTTTTTGC CACTCCTATG TATTGGCAGG AGAATCGACC 
AGAGAGAGTA AAACAGCAGG AAACATGAAA GCTCAAAGCA CTAGCTTGAC CAGCCTGGAA 
GAGAGAACAC ACCACAGAAC TGTAGGCCTG AAAGCGAAGG CGAAGACTCT AAGAGCGCCC 
CCTATGGATG GGGTCATGTC TCCAGAAACA GACAGTGGCT TTATGGGCTC TGAAAGCAGT 
CAGCTCACTG CTGCATTACA CAGTCCTCTC CACCAGAGGG CAGTAATAAG TTCCATCAGA 
CTGTCTGACC CTCACACAAA GGACACAGAG AGACCAGAGT CTGCTTCTCC TTCTGGGCAG 
CCTTTCATTG TCTCTCCTTC TCCATCCCAA CCTTCTCCAA ACACCACAAA AAAGCACATT 
GATACATCAG GAAAATGCAC AGGTCCTTAT AAGAAAATAG GAGGGGAGGA AAGGTGTTCT 
TTTTCCAGTC TTTCTTCCTC CTTATCCCCC CTTCACTGGC CACGTAGCAG CATTCTGCCC 
TGGACAAGCA GCCTGACCAG TCAATCAGAG CGTGGAGCTG ATGAGGAAGA AAAAGCACAG 
AAAGACAAAT ACCCACAACC GACCAATCAG CAACTCCGCT CTCACAGAAG CCCCTCCCTC 
CACAGGCCTT ATCACCACAG TTCTGCTCAG CTAACAAATC ACCAAAAGGC TCTTCAATCT 
CTGCAAAAAG AAGTGAACCG ATTGAAAGAG AGATTAGAGG GAAATCTGAG ACTATCAAAA 
CCTGCCAGAC CTATGAGAAC AGAAGTTTCT GCCTCTGAAG ACACCAGAGG TCACACACAT 
CCACAGACAT CAACACCTCA GAGGCATGAT TTAAGGTCTT CAAAGAGGAG GAGAAGGCAG 
ACAGAGAGAG CTGGCAATGG AGAAAAAGAG AGAGGAATTT CACCTAGACC AGTCCTCAGA 
CAAAGATCAG CATCTTTACC TCGACATCGG CCTGAAACTG ATATGACATC TGATTCTGAG 
CATGCTCAAT CTGAGCCAAG ACCATCAACG TCTAGATATG TCCCAGTGTC GCCTGTGAAA 
TCAAGAGGAA GAAGACAAAC CAGGAGATTT GAATGTCACA AAGAGGATAG TACATCAGGC 
AGATCTGACA ACAGCGATGA AGCAGAACCA AACGGAGGAG CAGAATCTCT GTGTCCTGAT 
TGCATATCCG ATCGTACCAG TGCTTTGACA CGTCTCCTGA GGAGCAGGAG CTCTCCACAT 
CTTGCACAGA CTTCCTGTAG CCATTGCCCA TTGTGTGGTG CATTACAAGC CATTCAAAGA 
GCCTCTAAGC CAGCTCCAGT CTCCAGTCCC AGAGCGAGTC AGCCAATGAG ATCCTTACAG 
AGAGAAAAAG GAGGCGTGAA CGTGACAGTG GCTCCTCCCC CTTTATTGCA GAGAGCTGTT 
CCAGTTGTCC CATATGTGCC TGTATATCCT TCAACTCTAT ATTTCTCCAG CCCAATGACT 
CCACCGGCAT ACCCACAACC CCTCAACATC TCATCAAATC CCGTCAGGCC TGAGAGGTCA 
GGGGTCAGAG GTCACCGCAG ACGCTCTTCC TCCATGGAGT ATCATCAGGA TTCTATGAGC 
AGCGCTTTGA CCCGTGCCAT CACTGCAGCG AGACACATGA GAGAAGCCTC ACAGCGCATG 
GCCAGCAGCC TCCACACTGC ATCCTGCAGC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293534.1
CDS	614..4969
Translation	MERSRGGTSTGVLVWTPAPQLSSPSASEGSDFSWDEEPDQDFQSQMDENGIIGLAERQGQQEREEEEVAEELLWDVKTPEPEHGPPPAVEEISCHLSELLDSEPLSQATGDHYYSPSLQEEHQTDDDRSVLSEDWADDEGGTGSPVKHKLSSFPERDTALDMTDEETEEKNTQEDAQRFMDQSFHVTEERAAVFQTLKTLDFSQEMSGIFKDHPISGVPEYSDQSDCEELDARFLPVSHHFSRNTSNPTSFPHLSSEELMNDCGIEAETLPELEIRSDSSLGTYQSPVSKTNHTFRSNQEEIKHKISPQTSPRSPVIKNSPEKIYQDATSGEKLDGRLHRPYHNLQPPTVSPRKTTSASCNTQVSIPAQAISKASVSPSERSPPPTPHRNSAIKNHRNCKTSHMDPDDIRKGQLNHALPDFSKVEPKVRFPKRSEYKPPKSRRPPHVRTSYPGLPVVFKSPAEIVKEVLLSSCDGTTGSPSSSETQKHLHNIVPEEFRCPQQASTLMQQLQEDYNRLLTKYAEAENTIDRLRLEAKVGLSSEPPKPSSTAVLSGVIQEGSKVITISFPQAQRAEFSTGSVHLTQQKELSENSRKAATRPSSVSSISSRRSGSLTPDHLTDTLSKQNQRFQLQVDAFESLLKGGKLKPCEQTKGLSTLAEGQESLERAYLNARVQYQMLQQRQGRHAIFDPDRDLEGQIFRSGMRLEELKEWLEQAEQNQPVSEPTLSPYPPSDVLSASMLEPEPVPEIPLSAVCPEACVGVEVSSVSGESDSGREEMEMLPFVLHPLDNKHQRVEKDFSELMDCYQSVKELPGTLDQAVTLKSHDLLDFGSSVPPGKKSVTDGQPLKTVNEREMRKPAQQQPAAGCVPPSCPSVSSQVCDSMETHPEAFCHSYVLAGESTRESKTAGNMKAQSTSLTSLEERTHHRTVGLKAKAKTLRAPPMDGVMSPETDSGFMGSESSQLTAALHSPLHQRAVISSIRLSDPHTKDTERPESASPSGQPFIVSPSPSQPSPNTTKKHIDTSGKCTGPYKKIGGEERCSFSSLSSSLSPLHWPRSSILPWTSSLTSQSERGADEEEKAQKDKYPQPTNQQLRSHRSPSLHRPYHHSSAQLTNHQKALQSLQKEVNRLKERLEGNLRLSKPARPMRTEVSASEDTRGHTHPQTSTPQRHDLRSSKRRRRQTERAGNGEKERGISPRPVLRQRSASLPRHRPETDMTSDSEHAQSEPRPSTSRYVPVSPVKSRGRRQTRRFECHKEDSTSGRSDNSDEAEPNGGAESLCPDCISDRTSALTRLLRSRSSPHLAQTSCSHCPLCGALQAIQRASKPAPVSSPRASQPMRSLQREKGGVNVTVAPPPLLQRAVPVVPYVPVYPSTLYFSSPMTPPAYPQPLNISSNPVRPERSGVRGHRRRSSSMEYHQDSMSSALTRAITAARHMREASQRMASSLHTASCSY

Target ORF information:

RefSeq Version	XM_009295259.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio AT-hook transcription factor (akna), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295259.3

ORF Insert Sequence:

ATGGAGCGGA GCAGAGGAGG CACCTCTACA GGGGTTCTGG TTTGGACACC TGCCCCACAG 
CTTTCCTCTC CCTCAGCCTC AGAGGGGTCA GACTTCAGCT GGGATGAAGA ACCGGACCAG 
GACTTCCAAA GCCAGATGGA TGAGAATGGG ATCATTGGTC TGGCAGAGAG GCAAGGGCAG 
CAGGAGAGAG AAGAGGAGGA GGTTGCAGAG GAGCTGTTGT GGGATGTGAA GACCCCTGAG 
CCTGAGCATG GGCCGCCGCC TGCAGTAGAG GAGATAAGCT GTCATCTGAG CGAACTGCTG 
GACTCTGAAC CGCTCTCTCA GGCCACTGGA GACCACTACT ACTCTCCATC ACTGCAGGAA 
GAACACCAAA CAGACGATGA TCGTAGTGTG TTGTCTGAGG ATTGGGCTGA TGACGAGGGT 
GGTACAGGGA GCCCAGTAAA GCACAAATTA AGCTCTTTCC CTGAGAGAGA CACTGCATTA 
GACATGACCG ATGAGGAGAC AGAAGAGAAA AACACACAAG AAGATGCTCA GAGATTCATG 
GATCAATCAT TTCATGTCAC AGAGGAGAGG GCAGCTGTGT TTCAAACTCT GAAAACCCTT 
GATTTCAGTC AGGAAATGAG TGGGATTTTC AAAGATCATC CAATTTCAGG AGTCCCAGAG 
TACTCTGATC AAAGTGACTG CGAGGAACTT GATGCCCGAT TTCTTCCAGT CTCGCATCAT 
TTCTCTAGAA ACACTTCCAA TCCCACCAGT TTTCCCCATT TATCCTCTGA GGAACTGATG 
AATGACTGTG GGATTGAAGC TGAGACGTTA CCTGAGCTAG AAATTAGGTC AGATTCATCT 
CTTGGCACTT ACCAGAGTCC TGTCTCTAAA ACAAACCACA CTTTCAGATC AAACCAGGAA 
GAGATTAAAC ACAAGATCTC ACCTCAAACT TCACCCAGAA GCCCTGTAAT AAAAAACAGC 
CCTGAAAAAA TATACCAAGA TGCTACGAGT GGCGAAAAAT TAGATGGAAG GTTACACAGG 
CCTTACCACA ATCTGCAGCC TCCAACAGTC TCTCCCAGAA AAACAACCTC TGCTTCCTGC 
AACACTCAGG TTTCCATTCC AGCGCAGGCT ATTTCTAAAG CAAGCGTCTC CCCCTCAGAG 
CGATCGCCTC CTCCTACACC TCACAGAAAT TCAGCAATAA AGAATCACAG AAACTGCAAA 
ACCTCTCACA TGGATCCAGA TGACATCAGG AAAGGACAGC TGAATCATGC CTTGCCTGAT 
TTCTCCAAAG TAGAGCCGAA GGTGCGTTTC CCAAAAAGAA GTGAATACAA ACCTCCCAAA 
AGCCGAAGAC CCCCTCATGT TCGAACATCA TACCCGGGTC TTCCAGTGGT GTTCAAATCT 
CCTGCTGAGA TTGTGAAAGA GGTCCTGTTG AGCAGCTGTG ATGGGACTAC TGGGAGTCCA 
TCCTCCAGCG AGACTCAGAA GCACCTGCAC AATATCGTCC CAGAAGAGTT TCGCTGCCCA 
CAGCAGGCCA GCACACTGAT GCAGCAGCTA CAGGAGGACT ATAACAGGCT TCTTACCAAG 
TATGCAGAAG CTGAAAATAC TATTGATCGA CTTCGCCTTG AAGCAAAGGT GGGTCTCTCT 
TCGGAACCAC CCAAACCCAG TAGTACAGCT GTCCTGTCGG GAGTTATTCA GGAAGGGTCA 
AAGGTCATAA CCATCAGTTT TCCTCAAGCA CAGAGGGCAG AGTTCAGTAC AGGTTCTGTT 
CATCTGACCC AACAGAAGGA GCTCTCAGAA AACTCCAGAA AAGCAGCAAC TCGGCCTTCT 
TCTGTAAGCT CTATCAGCTC GAGGAGGTCT GGATCTCTTA CTCCAGACCA TCTGACAGAC 
ACTCTGTCCA AACAGAACCA AAGATTTCAG CTACAGGTGG ATGCATTTGA GTCGCTTTTA 
AAGGGTGGGA AGCTCAAGCC ATGTGAGCAG ACAAAGGGTC TCTCCACACT GGCTGAGGGG 
CAGGAGTCTC TTGAGAGGGC TTATCTAAAT GCACGAGTCC AATATCAGAT GCTGCAGCAA 
CGACAAGGCA GACATGCCAT CTTTGACCCA GACAGGGATC TAGAGGGTCA GATCTTCAGG 
TCAGGGATGC GATTAGAGGA GCTGAAAGAG TGGCTGGAAC AAGCAGAACA GAACCAACCT 
GTTTCTGAAC CTACTTTATC CCCATATCCT CCTTCAGATG TCCTTTCTGC ATCCATGCTG 
GAGCCTGAAC CAGTGCCTGA GATCCCCCTG TCAGCAGTCT GTCCTGAGGC TTGTGTTGGT 
GTGGAGGTCA GTTCAGTCAG TGGAGAGAGT GATAGTGGTA GAGAGGAAAT GGAAATGCTT 
CCGTTTGTTC TTCATCCCCT CGATAACAAA CACCAACGTG TGGAGAAAGA TTTCAGTGAA 
CTCATGGACT GCTATCAGAG TGTTAAAGAG CTCCCCGGGA CACTGGATCA GGCTGTGACT 
TTAAAGAGCC ATGACTTGCT GGATTTTGGA AGCTCTGTTC CTCCTGGGAA AAAAAGTGTA 
ACGGACGGAC AACCACTCAA GACTGTTAAT GAGAGAGAGA TGAGAAAACC AGCTCAGCAG 
CAGCCGGCAG CAGGTTGTGT CCCTCCCTCA TGCCCATCTG TCAGTTCACA GGTGTGCGAC 
TCTATGGAAA CTCACCCAGA GGCTTTTTGC CACTCCTATG TATTGGCAGG AGAATCGACC 
AGAGAGAGTA AAACAGCAGG AAACATGAAA GCTCAAAGCA CTAGCTTGAC CAGCCTGGAA 
GAGAGAACAC ACCACAGAAC TGTAGGCCTG AAAGCGAAGG CGAAGACTCT AAGAGCGCCC 
CCTATGGATG GGGTCATGTC TCCAGAAACA GACAGTGGCT TTATGGGCTC TGAAAGCAGT 
CAGCTCACTG CTGCATTACA CAGTCCTCTC CACCAGAGGG CAGTAATAAG TTCCATCAGA 
CTGTCTGACC CTCACACAAA GGACACAGAG AGACCAGAGT CTGCTTCTCC TTCTGGGCAG 
CCTTTCATTG TCTCTCCTTC TCCATCCCAA CCTTCTCCAA ACACCACAAA AAAGCACATT 
GATACATCAG GAAAATGCAC AGGTCCTTAT AAGAAAATAG GAGGGGAGGA AAGGTGTTCT 
TTTTCCAGTC TTTCTTCCTC CTTATCCCCC CTTCACTGGC CACGTAGCAG CATTCTGCCC 
TGGACAAGCA GCCTGACCAG TCAATCAGAG CGTGGAGCTG ATGAGGAAGA AAAAGCACAG 
AAAGACAAAT ACCCACAACC GACCAATCAG CAACTCCGCT CTCACAGAAG CCCCTCCCTC 
CACAGGCCTT ATCACCACAG TTCTGCTCAG CTAACAAATC ACCAAAAGGC TCTTCAATCT 
CTGCAAAAAG AAGTGAACCG ATTGAAAGAG AGATTAGAGG GAAATCTGAG ACTATCAAAA 
CCTGCCAGAC CTATGAGAAC AGAAGTTTCT GCCTCTGAAG ACACCAGAGG TCACACACAT 
CCACAGACAT CAACACCTCA GAGGCATGAT TTAAGGTCTT CAAAGAGGAG GAGAAGGCAG 
ACAGAGAGAG CTGGCAATGG AGAAAAAGAG AGAGGAATTT CACCTAGACC AGTCCTCAGA 
CAAAGATCAG CATCTTTACC TCGACATCGG CCTGAAACTG ATATGACATC TGATTCTGAG 
CATGCTCAAT CTGAGCCAAG ACCATCAACG TCTAGATATG TCCCAGTGTC GCCTGTGAAA 
TCAAGAGGAA GAAGACAAAC CAGGAGATTT GAATGTCACA AAGAGGATAG TACATCAGGC 
AGATCTGACA ACAGCGATGA AGCAGAACCA AACGGAGGAG CAGAATCTCT GTGTCCTGAT 
TGCATATCCG ATCGTACCAG TGCTTTGACA CGTCTCCTGA GGAGCAGGAG CTCTCCACAT 
CTTGCACAGA CTTCCTGTAG CCATTGCCCA TTGTGTGGTG CATTACAAGC CATTCAAAGA 
GCCTCTAAGC CAGCTCCAGT CTCCAGTCCC AGAGCGAGTC AGCCAATGAG ATCCTTACAG 
AGAGAAAAAG GAGGCGTGAA CGTGACAGTG GCTCCTCCCC CTTTATTGCA GAGAGCTGTT 
CCAGTTGTCC CATATGTGCC TGTATATCCT TCAACTCTAT ATTTCTCCAG CCCAATGACT 
CCACCGGCAT ACCCACAACC CCTCAACATC TCATCAAATC CCGTCAGGCC TGAGAGGTCA 
GGGGTCAGAG GTCACCGCAG ACGCTCTTCC TCCATGGAGT ATCATCAGGA TTCTATGAGC 
AGCGCTTTGA CCCGTGCCAT CACTGCAGCG AGACACATGA GAGAAGCCTC ACAGCGCATG 
GCCAGCAGCC TCCACACTGC ATCCTGCAGC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

akna ( XM_009295259.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293534.1 Danio rerio AT-hook transcription factor (akna), transcript variant X7, mRNA.

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CloneID	ODa42303
Clone ID Related Accession (Same CDS sequence)	XM_009295259.3 , XM_009295259.2
Accession Version	XM_009295259.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	AT-hook-containing transcription factor-like isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295259.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGGA GCAGAGGAGG CACCTCTACA GGGGTTCTGG TTTGGACACC TGCCCCACAG 
CTTTCCTCTC CCTCAGCCTC AGAGGGGTCA GACTTCAGCT GGGATGAAGA ACCGGACCAG 
GACTTCCAAA GCCAGATGGA TGAGAATGGG ATCATTGGTC TGGCAGAGAG GCAAGGGCAG 
CAGGAGAGAG AAGAGGAGGA GGTTGCAGAG GAGCTGTTGT GGGATGTGAA GACCCCTGAG 
CCTGAGCATG GGCCGCCGCC TGCAGTAGAG GAGATAAGCT GTCATCTGAG CGAACTGCTG 
GACTCTGAAC CGCTCTCTCA GGCCACTGGA GACCACTACT ACTCTCCATC ACTGCAGGAA 
GAACACCAAA CAGACGATGA TCGTAGTGTG TTGTCTGAGG ATTGGGCTGA TGACGAGGGT 
GGTACAGGGA GCCCAGTAAA GCACAAATTA AGCTCTTTCC CTGAGAGAGA CACTGCATTA 
GACATGACCG ATGAGGAGAC AGAAGAGAAA AACACACAAG AAGATGCTCA GAGATTCATG 
GATCAATCAT TTCATGTCAC AGAGGAGAGG GCAGCTGTGT TTCAAACTCT GAAAACCCTT 
GATTTCAGTC AGGAAATGAG TGGGATTTTC AAAGATCATC CAATTTCAGG AGTCCCAGAG 
TACTCTGATC AAAGTGACTG CGAGGAACTT GATGCCCGAT TTCTTCCAGT CTCGCATCAT 
TTCTCTAGAA ACACTTCCAA TCCCACCAGT TTTCCCCATT TATCCTCTGA GGAACTGATG 
AATGACTGTG GGATTGAAGC TGAGACGTTA CCTGAGCTAG AAATTAGGTC AGATTCATCT 
CTTGGCACTT ACCAGAGTCC TGTCTCTAAA ACAAACCACA CTTTCAGATC AAACCAGGAA 
GAGATTAAAC ACAAGATCTC ACCTCAAACT TCACCCAGAA GCCCTGTAAT AAAAAACAGC 
CCTGAAAAAA TATACCAAGA TGCTACGAGT GGCGAAAAAT TAGATGGAAG GTTACACAGG 
CCTTACCACA ATCTGCAGCC TCCAACAGTC TCTCCCAGAA AAACAACCTC TGCTTCCTGC 
AACACTCAGG TTTCCATTCC AGCGCAGGCT ATTTCTAAAG CAAGCGTCTC CCCCTCAGAG 
CGATCGCCTC CTCCTACACC TCACAGAAAT TCAGCAATAA AGAATCACAG AAACTGCAAA 
ACCTCTCACA TGGATCCAGA TGACATCAGG AAAGGACAGC TGAATCATGC CTTGCCTGAT 
TTCTCCAAAG TAGAGCCGAA GGTGCGTTTC CCAAAAAGAA GTGAATACAA ACCTCCCAAA 
AGCCGAAGAC CCCCTCATGT TCGAACATCA TACCCGGGTC TTCCAGTGGT GTTCAAATCT 
CCTGCTGAGA TTGTGAAAGA GGTCCTGTTG AGCAGCTGTG ATGGGACTAC TGGGAGTCCA 
TCCTCCAGCG AGACTCAGAA GCACCTGCAC AATATCGTCC CAGAAGAGTT TCGCTGCCCA 
CAGCAGGCCA GCACACTGAT GCAGCAGCTA CAGGAGGACT ATAACAGGCT TCTTACCAAG 
TATGCAGAAG CTGAAAATAC TATTGATCGA CTTCGCCTTG AAGCAAAGGT GGGTCTCTCT 
TCGGAACCAC CCAAACCCAG TAGTACAGCT GTCCTGTCGG GAGTTATTCA GGAAGGGTCA 
AAGGTCATAA CCATCAGTTT TCCTCAAGCA CAGAGGGCAG AGTTCAGTAC AGGTTCTGTT 
CATCTGACCC AACAGAAGGA GCTCTCAGAA AACTCCAGAA AAGCAGCAAC TCGGCCTTCT 
TCTGTAAGCT CTATCAGCTC GAGGAGGTCT GGATCTCTTA CTCCAGACCA TCTGACAGAC 
ACTCTGTCCA AACAGAACCA AAGATTTCAG CTACAGGTGG ATGCATTTGA GTCGCTTTTA 
AAGGGTGGGA AGCTCAAGCC ATGTGAGCAG ACAAAGGGTC TCTCCACACT GGCTGAGGGG 
CAGGAGTCTC TTGAGAGGGC TTATCTAAAT GCACGAGTCC AATATCAGAT GCTGCAGCAA 
CGACAAGGCA GACATGCCAT CTTTGACCCA GACAGGGATC TAGAGGGTCA GATCTTCAGG 
TCAGGGATGC GATTAGAGGA GCTGAAAGAG TGGCTGGAAC AAGCAGAACA GAACCAACCT 
GTTTCTGAAC CTACTTTATC CCCATATCCT CCTTCAGATG TCCTTTCTGC ATCCATGCTG 
GAGCCTGAAC CAGTGCCTGA GATCCCCCTG TCAGCAGTCT GTCCTGAGGC TTGTGTTGGT 
GTGGAGGTCA GTTCAGTCAG TGGAGAGAGT GATAGTGGTA GAGAGGAAAT GGAAATGCTT 
CCGTTTGTTC TTCATCCCCT CGATAACAAA CACCAACGTG TGGAGAAAGA TTTCAGTGAA 
CTCATGGACT GCTATCAGAG TGTTAAAGAG CTCCCCGGGA CACTGGATCA GGCTGTGACT 
TTAAAGAGCC ATGACTTGCT GGATTTTGGA AGCTCTGTTC CTCCTGGGAA AAAAAGTGTA 
ACGGACGGAC AACCACTCAA GACTGTTAAT GAGAGAGAGA TGAGAAAACC AGCTCAGCAG 
CAGCCGGCAG CAGGTTGTGT CCCTCCCTCA TGCCCATCTG TCAGTTCACA GGTGTGCGAC 
TCTATGGAAA CTCACCCAGA GGCTTTTTGC CACTCCTATG TATTGGCAGG AGAATCGACC 
AGAGAGAGTA AAACAGCAGG AAACATGAAA GCTCAAAGCA CTAGCTTGAC CAGCCTGGAA 
GAGAGAACAC ACCACAGAAC TGTAGGCCTG AAAGCGAAGG CGAAGACTCT AAGAGCGCCC 
CCTATGGATG GGGTCATGTC TCCAGAAACA GACAGTGGCT TTATGGGCTC TGAAAGCAGT 
CAGCTCACTG CTGCATTACA CAGTCCTCTC CACCAGAGGG CAGTAATAAG TTCCATCAGA 
CTGTCTGACC CTCACACAAA GGACACAGAG AGACCAGAGT CTGCTTCTCC TTCTGGGCAG 
CCTTTCATTG TCTCTCCTTC TCCATCCCAA CCTTCTCCAA ACACCACAAA AAAGCACATT 
GATACATCAG GAAAATGCAC AGGTCCTTAT AAGAAAATAG GAGGGGAGGA AAGGTGTTCT 
TTTTCCAGTC TTTCTTCCTC CTTATCCCCC CTTCACTGGC CACGTAGCAG CATTCTGCCC 
TGGACAAGCA GCCTGACCAG TCAATCAGAG CGTGGAGCTG ATGAGGAAGA AAAAGCACAG 
AAAGACAAAT ACCCACAACC GACCAATCAG CAACTCCGCT CTCACAGAAG CCCCTCCCTC 
CACAGGCCTT ATCACCACAG TTCTGCTCAG CTAACAAATC ACCAAAAGGC TCTTCAATCT 
CTGCAAAAAG AAGTGAACCG ATTGAAAGAG AGATTAGAGG GAAATCTGAG ACTATCAAAA 
CCTGCCAGAC CTATGAGAAC AGAAGTTTCT GCCTCTGAAG ACACCAGAGG TCACACACAT 
CCACAGACAT CAACACCTCA GAGGCATGAT TTAAGGTCTT CAAAGAGGAG GAGAAGGCAG 
ACAGAGAGAG CTGGCAATGG AGAAAAAGAG AGAGGAATTT CACCTAGACC AGTCCTCAGA 
CAAAGATCAG CATCTTTACC TCGACATCGG CCTGAAACTG ATATGACATC TGATTCTGAG 
CATGCTCAAT CTGAGCCAAG ACCATCAACG TCTAGATATG TCCCAGTGTC GCCTGTGAAA 
TCAAGAGGAA GAAGACAAAC CAGGAGATTT GAATGTCACA AAGAGGATAG TACATCAGGC 
AGATCTGACA ACAGCGATGA AGCAGAACCA AACGGAGGAG CAGAATCTCT GTGTCCTGAT 
TGCATATCCG ATCGTACCAG TGCTTTGACA CGTCTCCTGA GGAGCAGGAG CTCTCCACAT 
CTTGCACAGA CTTCCTGTAG CCATTGCCCA TTGTGTGGTG CATTACAAGC CATTCAAAGA 
GCCTCTAAGC CAGCTCCAGT CTCCAGTCCC AGAGCGAGTC AGCCAATGAG ATCCTTACAG 
AGAGAAAAAG GAGGCGTGAA CGTGACAGTG GCTCCTCCCC CTTTATTGCA GAGAGCTGTT 
CCAGTTGTCC CATATGTGCC TGTATATCCT TCAACTCTAT ATTTCTCCAG CCCAATGACT 
CCACCGGCAT ACCCACAACC CCTCAACATC TCATCAAATC CCGTCAGGCC TGAGAGGTCA 
GGGGTCAGAG GTCACCGCAG ACGCTCTTCC TCCATGGAGT ATCATCAGGA TTCTATGAGC 
AGCGCTTTGA CCCGTGCCAT CACTGCAGCG AGACACATGA GAGAAGCCTC ACAGCGCATG 
GCCAGCAGCC TCCACACTGC ATCCTGCAGC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293534.1
CDS	617..4972
Translation	MERSRGGTSTGVLVWTPAPQLSSPSASEGSDFSWDEEPDQDFQSQMDENGIIGLAERQGQQEREEEEVAEELLWDVKTPEPEHGPPPAVEEISCHLSELLDSEPLSQATGDHYYSPSLQEEHQTDDDRSVLSEDWADDEGGTGSPVKHKLSSFPERDTALDMTDEETEEKNTQEDAQRFMDQSFHVTEERAAVFQTLKTLDFSQEMSGIFKDHPISGVPEYSDQSDCEELDARFLPVSHHFSRNTSNPTSFPHLSSEELMNDCGIEAETLPELEIRSDSSLGTYQSPVSKTNHTFRSNQEEIKHKISPQTSPRSPVIKNSPEKIYQDATSGEKLDGRLHRPYHNLQPPTVSPRKTTSASCNTQVSIPAQAISKASVSPSERSPPPTPHRNSAIKNHRNCKTSHMDPDDIRKGQLNHALPDFSKVEPKVRFPKRSEYKPPKSRRPPHVRTSYPGLPVVFKSPAEIVKEVLLSSCDGTTGSPSSSETQKHLHNIVPEEFRCPQQASTLMQQLQEDYNRLLTKYAEAENTIDRLRLEAKVGLSSEPPKPSSTAVLSGVIQEGSKVITISFPQAQRAEFSTGSVHLTQQKELSENSRKAATRPSSVSSISSRRSGSLTPDHLTDTLSKQNQRFQLQVDAFESLLKGGKLKPCEQTKGLSTLAEGQESLERAYLNARVQYQMLQQRQGRHAIFDPDRDLEGQIFRSGMRLEELKEWLEQAEQNQPVSEPTLSPYPPSDVLSASMLEPEPVPEIPLSAVCPEACVGVEVSSVSGESDSGREEMEMLPFVLHPLDNKHQRVEKDFSELMDCYQSVKELPGTLDQAVTLKSHDLLDFGSSVPPGKKSVTDGQPLKTVNEREMRKPAQQQPAAGCVPPSCPSVSSQVCDSMETHPEAFCHSYVLAGESTRESKTAGNMKAQSTSLTSLEERTHHRTVGLKAKAKTLRAPPMDGVMSPETDSGFMGSESSQLTAALHSPLHQRAVISSIRLSDPHTKDTERPESASPSGQPFIVSPSPSQPSPNTTKKHIDTSGKCTGPYKKIGGEERCSFSSLSSSLSPLHWPRSSILPWTSSLTSQSERGADEEEKAQKDKYPQPTNQQLRSHRSPSLHRPYHHSSAQLTNHQKALQSLQKEVNRLKERLEGNLRLSKPARPMRTEVSASEDTRGHTHPQTSTPQRHDLRSSKRRRRQTERAGNGEKERGISPRPVLRQRSASLPRHRPETDMTSDSEHAQSEPRPSTSRYVPVSPVKSRGRRQTRRFECHKEDSTSGRSDNSDEAEPNGGAESLCPDCISDRTSALTRLLRSRSSPHLAQTSCSHCPLCGALQAIQRASKPAPVSSPRASQPMRSLQREKGGVNVTVAPPPLLQRAVPVVPYVPVYPSTLYFSSPMTPPAYPQPLNISSNPVRPERSGVRGHRRRSSSMEYHQDSMSSALTRAITAARHMREASQRMASSLHTASCSY

Target ORF information:

RefSeq Version	XM_009295259.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio AT-hook transcription factor (akna), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295259.2

ORF Insert Sequence:

ATGGAGCGGA GCAGAGGAGG CACCTCTACA GGGGTTCTGG TTTGGACACC TGCCCCACAG 
CTTTCCTCTC CCTCAGCCTC AGAGGGGTCA GACTTCAGCT GGGATGAAGA ACCGGACCAG 
GACTTCCAAA GCCAGATGGA TGAGAATGGG ATCATTGGTC TGGCAGAGAG GCAAGGGCAG 
CAGGAGAGAG AAGAGGAGGA GGTTGCAGAG GAGCTGTTGT GGGATGTGAA GACCCCTGAG 
CCTGAGCATG GGCCGCCGCC TGCAGTAGAG GAGATAAGCT GTCATCTGAG CGAACTGCTG 
GACTCTGAAC CGCTCTCTCA GGCCACTGGA GACCACTACT ACTCTCCATC ACTGCAGGAA 
GAACACCAAA CAGACGATGA TCGTAGTGTG TTGTCTGAGG ATTGGGCTGA TGACGAGGGT 
GGTACAGGGA GCCCAGTAAA GCACAAATTA AGCTCTTTCC CTGAGAGAGA CACTGCATTA 
GACATGACCG ATGAGGAGAC AGAAGAGAAA AACACACAAG AAGATGCTCA GAGATTCATG 
GATCAATCAT TTCATGTCAC AGAGGAGAGG GCAGCTGTGT TTCAAACTCT GAAAACCCTT 
GATTTCAGTC AGGAAATGAG TGGGATTTTC AAAGATCATC CAATTTCAGG AGTCCCAGAG 
TACTCTGATC AAAGTGACTG CGAGGAACTT GATGCCCGAT TTCTTCCAGT CTCGCATCAT 
TTCTCTAGAA ACACTTCCAA TCCCACCAGT TTTCCCCATT TATCCTCTGA GGAACTGATG 
AATGACTGTG GGATTGAAGC TGAGACGTTA CCTGAGCTAG AAATTAGGTC AGATTCATCT 
CTTGGCACTT ACCAGAGTCC TGTCTCTAAA ACAAACCACA CTTTCAGATC AAACCAGGAA 
GAGATTAAAC ACAAGATCTC ACCTCAAACT TCACCCAGAA GCCCTGTAAT AAAAAACAGC 
CCTGAAAAAA TATACCAAGA TGCTACGAGT GGCGAAAAAT TAGATGGAAG GTTACACAGG 
CCTTACCACA ATCTGCAGCC TCCAACAGTC TCTCCCAGAA AAACAACCTC TGCTTCCTGC 
AACACTCAGG TTTCCATTCC AGCGCAGGCT ATTTCTAAAG CAAGCGTCTC CCCCTCAGAG 
CGATCGCCTC CTCCTACACC TCACAGAAAT TCAGCAATAA AGAATCACAG AAACTGCAAA 
ACCTCTCACA TGGATCCAGA TGACATCAGG AAAGGACAGC TGAATCATGC CTTGCCTGAT 
TTCTCCAAAG TAGAGCCGAA GGTGCGTTTC CCAAAAAGAA GTGAATACAA ACCTCCCAAA 
AGCCGAAGAC CCCCTCATGT TCGAACATCA TACCCGGGTC TTCCAGTGGT GTTCAAATCT 
CCTGCTGAGA TTGTGAAAGA GGTCCTGTTG AGCAGCTGTG ATGGGACTAC TGGGAGTCCA 
TCCTCCAGCG AGACTCAGAA GCACCTGCAC AATATCGTCC CAGAAGAGTT TCGCTGCCCA 
CAGCAGGCCA GCACACTGAT GCAGCAGCTA CAGGAGGACT ATAACAGGCT TCTTACCAAG 
TATGCAGAAG CTGAAAATAC TATTGATCGA CTTCGCCTTG AAGCAAAGGT GGGTCTCTCT 
TCGGAACCAC CCAAACCCAG TAGTACAGCT GTCCTGTCGG GAGTTATTCA GGAAGGGTCA 
AAGGTCATAA CCATCAGTTT TCCTCAAGCA CAGAGGGCAG AGTTCAGTAC AGGTTCTGTT 
CATCTGACCC AACAGAAGGA GCTCTCAGAA AACTCCAGAA AAGCAGCAAC TCGGCCTTCT 
TCTGTAAGCT CTATCAGCTC GAGGAGGTCT GGATCTCTTA CTCCAGACCA TCTGACAGAC 
ACTCTGTCCA AACAGAACCA AAGATTTCAG CTACAGGTGG ATGCATTTGA GTCGCTTTTA 
AAGGGTGGGA AGCTCAAGCC ATGTGAGCAG ACAAAGGGTC TCTCCACACT GGCTGAGGGG 
CAGGAGTCTC TTGAGAGGGC TTATCTAAAT GCACGAGTCC AATATCAGAT GCTGCAGCAA 
CGACAAGGCA GACATGCCAT CTTTGACCCA GACAGGGATC TAGAGGGTCA GATCTTCAGG 
TCAGGGATGC GATTAGAGGA GCTGAAAGAG TGGCTGGAAC AAGCAGAACA GAACCAACCT 
GTTTCTGAAC CTACTTTATC CCCATATCCT CCTTCAGATG TCCTTTCTGC ATCCATGCTG 
GAGCCTGAAC CAGTGCCTGA GATCCCCCTG TCAGCAGTCT GTCCTGAGGC TTGTGTTGGT 
GTGGAGGTCA GTTCAGTCAG TGGAGAGAGT GATAGTGGTA GAGAGGAAAT GGAAATGCTT 
CCGTTTGTTC TTCATCCCCT CGATAACAAA CACCAACGTG TGGAGAAAGA TTTCAGTGAA 
CTCATGGACT GCTATCAGAG TGTTAAAGAG CTCCCCGGGA CACTGGATCA GGCTGTGACT 
TTAAAGAGCC ATGACTTGCT GGATTTTGGA AGCTCTGTTC CTCCTGGGAA AAAAAGTGTA 
ACGGACGGAC AACCACTCAA GACTGTTAAT GAGAGAGAGA TGAGAAAACC AGCTCAGCAG 
CAGCCGGCAG CAGGTTGTGT CCCTCCCTCA TGCCCATCTG TCAGTTCACA GGTGTGCGAC 
TCTATGGAAA CTCACCCAGA GGCTTTTTGC CACTCCTATG TATTGGCAGG AGAATCGACC 
AGAGAGAGTA AAACAGCAGG AAACATGAAA GCTCAAAGCA CTAGCTTGAC CAGCCTGGAA 
GAGAGAACAC ACCACAGAAC TGTAGGCCTG AAAGCGAAGG CGAAGACTCT AAGAGCGCCC 
CCTATGGATG GGGTCATGTC TCCAGAAACA GACAGTGGCT TTATGGGCTC TGAAAGCAGT 
CAGCTCACTG CTGCATTACA CAGTCCTCTC CACCAGAGGG CAGTAATAAG TTCCATCAGA 
CTGTCTGACC CTCACACAAA GGACACAGAG AGACCAGAGT CTGCTTCTCC TTCTGGGCAG 
CCTTTCATTG TCTCTCCTTC TCCATCCCAA CCTTCTCCAA ACACCACAAA AAAGCACATT 
GATACATCAG GAAAATGCAC AGGTCCTTAT AAGAAAATAG GAGGGGAGGA AAGGTGTTCT 
TTTTCCAGTC TTTCTTCCTC CTTATCCCCC CTTCACTGGC CACGTAGCAG CATTCTGCCC 
TGGACAAGCA GCCTGACCAG TCAATCAGAG CGTGGAGCTG ATGAGGAAGA AAAAGCACAG 
AAAGACAAAT ACCCACAACC GACCAATCAG CAACTCCGCT CTCACAGAAG CCCCTCCCTC 
CACAGGCCTT ATCACCACAG TTCTGCTCAG CTAACAAATC ACCAAAAGGC TCTTCAATCT 
CTGCAAAAAG AAGTGAACCG ATTGAAAGAG AGATTAGAGG GAAATCTGAG ACTATCAAAA 
CCTGCCAGAC CTATGAGAAC AGAAGTTTCT GCCTCTGAAG ACACCAGAGG TCACACACAT 
CCACAGACAT CAACACCTCA GAGGCATGAT TTAAGGTCTT CAAAGAGGAG GAGAAGGCAG 
ACAGAGAGAG CTGGCAATGG AGAAAAAGAG AGAGGAATTT CACCTAGACC AGTCCTCAGA 
CAAAGATCAG CATCTTTACC TCGACATCGG CCTGAAACTG ATATGACATC TGATTCTGAG 
CATGCTCAAT CTGAGCCAAG ACCATCAACG TCTAGATATG TCCCAGTGTC GCCTGTGAAA 
TCAAGAGGAA GAAGACAAAC CAGGAGATTT GAATGTCACA AAGAGGATAG TACATCAGGC 
AGATCTGACA ACAGCGATGA AGCAGAACCA AACGGAGGAG CAGAATCTCT GTGTCCTGAT 
TGCATATCCG ATCGTACCAG TGCTTTGACA CGTCTCCTGA GGAGCAGGAG CTCTCCACAT 
CTTGCACAGA CTTCCTGTAG CCATTGCCCA TTGTGTGGTG CATTACAAGC CATTCAAAGA 
GCCTCTAAGC CAGCTCCAGT CTCCAGTCCC AGAGCGAGTC AGCCAATGAG ATCCTTACAG 
AGAGAAAAAG GAGGCGTGAA CGTGACAGTG GCTCCTCCCC CTTTATTGCA GAGAGCTGTT 
CCAGTTGTCC CATATGTGCC TGTATATCCT TCAACTCTAT ATTTCTCCAG CCCAATGACT 
CCACCGGCAT ACCCACAACC CCTCAACATC TCATCAAATC CCGTCAGGCC TGAGAGGTCA 
GGGGTCAGAG GTCACCGCAG ACGCTCTTCC TCCATGGAGT ATCATCAGGA TTCTATGAGC 
AGCGCTTTGA CCCGTGCCAT CACTGCAGCG AGACACATGA GAGAAGCCTC ACAGCGCATG 
GCCAGCAGCC TCCACACTGC ATCCTGCAGC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	akna
Entrez Gene ID	556113
Full Name	AT-hook transcription factor
Synonyms	si:ch211-86k6.3,si:ch211-86k6.4,si:ch211-86k6.5
General protein information	Preferred Names AT-hook transcription factor Names AT-hook-containing transcription factor-like
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

akna cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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ORF Insert Sequence:

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Target ORF information:

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Target ORF information:

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