neurl4 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	neurl4
Entrez Gene ID	555331
Full Name	neuralized E3 ubiquitin protein ligase 4
Synonyms	sb:eu380
General protein information	Preferred Names neuralized E3 ubiquitin protein ligase 4 Names neuralized E3 ubiquitin protein ligase 4 neuralized-like protein 4 neuralized homolog 4
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_677822.8	XP_682914.5	neuralized-like protein 4 isoform X1
XM_005156224.4	XP_005156281.1	neuralized-like protein 4 isoform X2
XM_005156224.3	XP_005156281.1	neuralized-like protein 4 isoform X2
XM_021480311.1	XP_021335986.1	neuralized-like protein 4 isoform X2
XM_677822.9	XP_682914.5	neuralized-like protein 4 isoform X1
XM_021480310.1	XP_021335985.1	neuralized-like protein 4 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NEURL4	XP_003315396.1
Mus musculus (house mouse)	Neurl4	NP_001013432.1
Canis lupus familiaris (dog)	NEURL4	XP_005620031.1
Danio rerio (zebrafish)	neurl4	XP_682914.5
Drosophila melanogaster (fruit fly)	blue	NP_648688.2
Homo sapiens (human)	NEURL4	NP_115818.2
	LOC714906	XP_002808159.1
Bos taurus (cattle)	NEURL4	NP_001179590.1
Rattus norvegicus (Norway rat)	Neurl4	NP_001100483.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011965	XP_320568.4
Xenopus tropicalis (tropical clawed frog)	neurl4	NP_001090721.1

Gene Ontology

The following neurl4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the neurl4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa17890	XM_021480310.1 Latest version!	Danio rerio neuralized E3 ubiquitin protein ligase 4 (neurl4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa17890	XM_677822.9 Latest version!	Danio rerio neuralized E3 ubiquitin protein ligase 4 (neurl4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa17891	XM_021480311.1 Latest version!	Danio rerio neuralized E3 ubiquitin protein ligase 4 (neurl4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa17891	XM_005156224.4 Latest version!	Danio rerio neuralized E3 ubiquitin protein ligase 4 (neurl4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
ODa17891	XM_005156224.3	Danio rerio neuralized E3 ubiquitin protein ligase 4 (neurl4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa17890	XM_677822.8	Danio rerio neuralized E3 ubiquitin protein ligase 4 (neurl4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

neurl4 ( XM_677822.8 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_682914.5 Danio rerio neuralized E3 ubiquitin protein ligase 4 (neurl4), transcript variant X1, mRNA.

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CloneID	ODa17890
Clone ID Related Accession (Same CDS sequence)	XM_677822.8 , XM_677822.9 , XM_021480310.1
Accession Version	XM_677822.8	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	neuralized-like protein 4 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_677822.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG AGCTGCATCC GCGCAGCGGA AAACTGATCG GTTTGTCGAA CTCCAACCGA 
ACCGCTCAAC GGAATCAGCC GGTACAGGAA TTCAATCACG GCCTGGTCCT CAGTCGGGAG 
CCGCTGAGGG ACCGCGATGT CTTCACTGTC CGCATCGACA AGAAGGTGAA CTCTTGGAGC 
GGCTCTATAG AAATCGGTGT GACGGCGCTG GACCCAGCCG GTCTGGAATT TCCCAGCAGC 
GCCACAGGGC TGAAAGGTGG CTCGTGGATT GTGTCTGGCT GCTCGGTGCT GAAGGACGGC 
CGTTCGGTCC TGGAGGAGTA CGGTCGGGAT CTTGACCAGC TTGGGGAAGG AGACCGTGTG 
GGCATCCAGC GGAATGGGAA AGGAGAGCTA CACCTGTGGG TGAATGGGCA GGACTGCGGC 
GCTGCTGCTA GTGGACTTCC ATCTCGACTG TGGGCTGTGG TGGACCTCTA TGGGAAGTGC 
ACACAAGTGA CTGTGGTGTC CTGTGAACCG CCATCTGAAA TGGAGGGTGA CGAGGATGAA 
GAGGTGGAGG ATGAGATTGT GTCGACGCAG GCAGTGTCCA TTATGGCAGA TGCCTGTCGT 
AGTCATGATG ATAGAGCTGC TGTACTGACT GCAGCTGCTG CTACTACAGA GCGGATGAAC 
GGATCAGCGG AGGAAGTGCC TGAAGTGTCA AGGACGCACA GTCGGCCGGA CAAATTTCCA 
AATAACTTTG AGCCTGATAG TGTTTTGACT GAGCATCAGT TGTTTGATGT GTTCAACAAT 
GCCATTGTGT CTCTGTATCG CTCGGAGGAT GAGGGGGCAG AGGATTTGAA TGTTGGGGGT 
GGAGGTGGCG GTTCTGACTC CTCAAGGGGC GGCACAGGAA GTAACGGTGG AGGCAGAGGA 
ACAGGAAGTG ACAGTGGCAT TGGCGGCGGA GGTGGTGGTG GAGGCAGTAG CAGCAATAGC 
AGCCCGGCGG GCAACGCTGG TGGGGCAGCA GGACTAGGGG TGGGCACTGG TGGAATGACG 
ACCAATGATG CACTGCTGTT TCATGAGAAA TGTGGGACGC TGATCAAACT GAGCAACAAT 
AATAAGACAG CAGAAAGAAG GAGACCGCTG GATGAGTTCA ATAATGGAGT CGTCATGACC 
AATCGACCAC TCAGGCACAA CGAAATGTTT GAGATTCGCA TTGACAAACT GGTGGATAAA 
TGGTCTGGTT CCATTGAGAT TGGAGTGACA ACCCACAACC CAAATAATCT TGATTACCCC 
GCAACAATGA CCAATTTGCG CTCAGGCACA ATAATGATGA GTGGCTGTGG GATTTTAACC 
AATGGGAAAG GGACTCGTCG GGAATACTGC GAATTCAGCC TGGATGAACT ACAGGAAGGA 
GATCACATTG GGCTGATGAG GAAAGCCAGT GGAGCACTGC ACTTCTACAT TAATGGCATT 
GATCAAGGGG TTGCAGCCAA CCAGACACCT GGCGTAGTGT ATGGAGTGGT GGATCTCTAT 
GGCATGGCGG TTAAAGTCAC CATTGTGCAC AACCATAACC ACAGTGATCG GTTGCGCCGC 
AATAACGCCA TCATGAGGGC ACTGTCACCT GACGTGGGTC GACCCCGGCC TGCACTCTCA 
TTAACCCCTG AGCCTGATAT CCCAGACCGC TTGCTCTTCC ATCCAAATTG TGGGCAGAAG 
GCTGCAATCA TCAGCGATGG ACGGACTGCT TTACGACCTC ATGCGACGGA TGACTTCAAC 
CATGGTGTTG TGCTTAGCAA TCGTCCATTG CACTCCAATG AGGTTTTCCA GGTGCGCATT 
GACAAAATGG TGGACAAATG GGCTGGCTCC ATAGAGATTG GTGTGACGAC TCATAACCCT 
GCCTATCTTC AGTTACCATC AACTATGACC AACCTGCGCT CGGGAACCTG GATGATGACT 
GGAAATGGGG TCATGCACAA TGGAACTACA ATTCTGGATG AATATGGGCA CAATTTGGAC 
CGTCTAAAAG CTGGAGATAC GGTGGGAGTT GTCCGAAAGG AAGATGGGAG TTTGCATTTC 
TTTGTGAATG GAGTGCCTCA AGGTCCTGCT GCCTGGAATG TTCCGCCCAA CGTGTATGCA 
GTAGTGGATC TGTATGGACA GGCTGCTCAG GCCACCATCA TGGATGATAT GGCTGATCTC 
CCTCCTTTGC CTGACGACAG CTCAGAGGGT CCCACTGCCA TGTCTCCTGG TAGCCCGTGT 
TCAATTTCAG GAGCCACAGC TGCAAATGAC CTGCGCTTTC ATCAGCTGCA TGGCACTAAC 
GCAGTCATCA CCAACGGTGG CCGAACAGCA CTTAGGCAGA ACTGCCGCAG CGAGTTTAAT 
GATGCCATCG TCATTTCAAA CAGGTGCCTT CGAGAAGGAG AGCTCTTTGA AATTGTGATT 
CAGAAAATGG TGGATCGCTG GTCGGGGTCT ATAGAAGCAG GAGTGACAGC GATCAGACCC 
GAAGAACTGG AGTTTCCAAA TACGATGACT GACATTGACT ATGACACATG GATGCTGAGC 
GGCACAGCAA TCATGCAGGA TGGCAATACC ATGAGAAATA ACTATGGCTG TGACTTAGAC 
TCTCTGACTA CTGCCTCCAG GATTGGGATG ATGCGAACAG CAACTGGAGA CCTGCATTAC 
TTCATCAACG GCCTCGACCA GGGTGTAGCT TGTACTGGGC TGCCATCAGA AGTGTATGCT 
GTGGTCGATC TTTATGGTCA GTGTGTGCAA GTGTCCATTA CAAGCTCATC CGGTCCACTG 
GACAACAGTC TGTGTACCAG TAACATCACA GAAAAGAGCT TCCCCATACA CTCCCCGGTG 
GCAGGAGTAG CTCATCGCCT GCACAGTAAA CATGGTAAAA ACGTGGTGTT ATTAGGAGAT 
GGATGCCAAG CGCTGAGGGT CGGAGGTTAT GCCCATGGAA TTGTCTTTAG TGCAAAAGAG 
CTCAAAACAG ATGAAGTGTT TGAGGTGAAA ATTAACAAGG TGGATGAGAG GTGGTCTGGT 
TCTCTTCATG TGGGGCTGAC CACCCTGCAG CCCCCTGACC TGCCATCATG CCCTCTCAGC 
GGCCTCTCGC CTTCACTCAC TCAGCTCAGG ACAAAGGTCA CCTGGGTGCT GGCCGGCTCT 
GAGGTCAGAC GCAACGGAGT ACTGCAGAGA CAGAACTATG GCTGCTCTCT GGATCGCCTC 
ACGGTTGGAA ATCGTGTCGG GGTGAAGAGA TGCAGTGACG ACACCATGCA CATTCTCATT 
GATGGAGAAG ATATGGGACC AGCAGCCACT GCAGTAGCAA AGAACGTGTA TGCTGTATTG 
GACCTGTATG GAAAGGTGAC GGCTGTGTCC ATCGTCAGTT CAACACTAGT TGAGGATTCT 
GAGAGTGTGA AAGCTCCCTC GCTCTCGTCC GACAGCTGCA GTGAAGGAGA AGAGGACTGC 
ACTCCTGTCA GAGAGTGTGA GAATGAGCCT CCATTGGTGC CCACAGTCAT GACGTTTTTG 
GAGAATCATG GGAAGAATAT CCAGCTGTCC AATCAAAATC TGACCGCCGC TCGGGTGTCC 
AGCTACAACC AGGGGCTTCT GGTCACAGCC CAAGCTCTGC CTCGCCAGCA GCTCTTCCAG 
TTTCAGATTG ACCGCTTGAA TCCCTCATGG ACGTCGTCTC TCTCCCTCGG TGTGATTGGT 
CATTCACCAG ACCGACTCAA CTTTCCTTCC ACAGCCTGCT GCCTTAAACG CTCAGTCTGG 
CTACTGCAAC GAGACTCTGT TTTCCACAAC TCGCTAAAGA TTTGTGAGAA CTATGGTCCA 
AACCTGGACA CCTGTCCAGA GGGGACAGTT TTGGGGCTGT TGGTGGATAG CAGTGGTTGT 
CTCCATTTGT TTGTGAATGG CATGGATCAG GGTGTAGCGG CACAGGACAT CCCGAGCCCC 
TGTTACGCCC TCATTGACCT TTACGGCCAG TGTGAACAGG TGACTATCGT GGCGAATCAT 
GTGCCGGTGG TTGGCGGTGA GCGAGGAGAC CTGCGCTGTC AAGGGGACAT GGAGAAGGCT 
GATATGGTGG ACGGTATAAA AGAGAGTGTC TGCTGGACTC CTCCTCCCGA GGTCAACCCT 
AATAAGACCT GCGAGTATCA GGCGCTGTGC TCCAGATTCA AAGACCTGCT CACACTGCCT 
GATGGTTACT TTAATGAGGA TGCCAAGTAT AACCTGTGTT ACTGTGAGTC CTGTCATAAA 
TTAAGGGGTG ATGAGGCCTA TTACAAGCGA GGAGAGCCCC CCAGAGACTA TGCTCTACCG 
TTCGGCTGGT GCCGCTTTGC ACTCCAGATC AAGACCCACT GTGAAGTCTC CAATGCCTTC 
AAGAAGTGGC ACATTGCGTA TCACGGCACA AGTGTCGGTT CTCTTAGACG CACCCTGGAC 
CACAACCAAC TGTTATCAGC AGAATCATCC TCTATTTTCT CGTCATCGTC GGTGAAGACG 
GAGGGTCATG GCAGCTACGG AGAGCCGGAG GAGAACAGCG CTCCAGAGAG AGACATTCCC 
AGAGTGCAGC TCTCACCAAC CATGCGATAT TCCGGCCTCG AGGTCTTCGC TCCGAAAGTG 
CAATTTCGAG ACCCGCGTTC GCTCCGCTGT CACCAGGCTC AGGTGGGCTT CCAGGTGTGT 
GTCCGGCCAG GCTCCTATAA AGTAGGTCCG TCGTCTTTAG GAATCAGCGA GCCGCTAGAC 
CCGCGATTCA GCAATGCAGA AATCGAATGG ATCACCAAGG AGAAAGGAGG CACGCTTCTA 
TATGGCCTGC TCATACGAGT TGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_682914.5
CDS	73..4839
Translation	MAAELHPRSGKLIGLSNSNRTAQRNQPVQEFNHGLVLSREPLRDRDVFTVRIDKKVNSWSGSIEIGVTALDPAGLEFPSSATGLKGGSWIVSGCSVLKDGRSVLEEYGRDLDQLGEGDRVGIQRNGKGELHLWVNGQDCGAAASGLPSRLWAVVDLYGKCTQVTVVSCEPPSEMEGDEDEEVEDEIVSTQAVSIMADACRSHDDRAAVLTAAAATTERMNGSAEEVPEVSRTHSRPDKFPNNFEPDSVLTEHQLFDVFNNAIVSLYRSEDEGAEDLNVGGGGGGSDSSRGGTGSNGGGRGTGSDSGIGGGGGGGGSSSNSSPAGNAGGAAGLGVGTGGMTTNDALLFHEKCGTLIKLSNNNKTAERRRPLDEFNNGVVMTNRPLRHNEMFEIRIDKLVDKWSGSIEIGVTTHNPNNLDYPATMTNLRSGTIMMSGCGILTNGKGTRREYCEFSLDELQEGDHIGLMRKASGALHFYINGIDQGVAANQTPGVVYGVVDLYGMAVKVTIVHNHNHSDRLRRNNAIMRALSPDVGRPRPALSLTPEPDIPDRLLFHPNCGQKAAIISDGRTALRPHATDDFNHGVVLSNRPLHSNEVFQVRIDKMVDKWAGSIEIGVTTHNPAYLQLPSTMTNLRSGTWMMTGNGVMHNGTTILDEYGHNLDRLKAGDTVGVVRKEDGSLHFFVNGVPQGPAAWNVPPNVYAVVDLYGQAAQATIMDDMADLPPLPDDSSEGPTAMSPGSPCSISGATAANDLRFHQLHGTNAVITNGGRTALRQNCRSEFNDAIVISNRCLREGELFEIVIQKMVDRWSGSIEAGVTAIRPEELEFPNTMTDIDYDTWMLSGTAIMQDGNTMRNNYGCDLDSLTTASRIGMMRTATGDLHYFINGLDQGVACTGLPSEVYAVVDLYGQCVQVSITSSSGPLDNSLCTSNITEKSFPIHSPVAGVAHRLHSKHGKNVVLLGDGCQALRVGGYAHGIVFSAKELKTDEVFEVKINKVDERWSGSLHVGLTTLQPPDLPSCPLSGLSPSLTQLRTKVTWVLAGSEVRRNGVLQRQNYGCSLDRLTVGNRVGVKRCSDDTMHILIDGEDMGPAATAVAKNVYAVLDLYGKVTAVSIVSSTLVEDSESVKAPSLSSDSCSEGEEDCTPVRECENEPPLVPTVMTFLENHGKNIQLSNQNLTAARVSSYNQGLLVTAQALPRQQLFQFQIDRLNPSWTSSLSLGVIGHSPDRLNFPSTACCLKRSVWLLQRDSVFHNSLKICENYGPNLDTCPEGTVLGLLVDSSGCLHLFVNGMDQGVAAQDIPSPCYALIDLYGQCEQVTIVANHVPVVGGERGDLRCQGDMEKADMVDGIKESVCWTPPPEVNPNKTCEYQALCSRFKDLLTLPDGYFNEDAKYNLCYCESCHKLRGDEAYYKRGEPPRDYALPFGWCRFALQIKTHCEVSNAFKKWHIAYHGTSVGSLRRTLDHNQLLSAESSSIFSSSSVKTEGHGSYGEPEENSAPERDIPRVQLSPTMRYSGLEVFAPKVQFRDPRSLRCHQAQVGFQVCVRPGSYKVGPSSLGISEPLDPRFSNAEIEWITKEKGGTLLYGLLIRVE

Target ORF information:

RefSeq Version	XM_677822.8
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neuralized E3 ubiquitin protein ligase 4 (neurl4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_677822.8

ORF Insert Sequence:

ATGGCGGCTG AGCTGCATCC GCGCAGCGGA AAACTGATCG GTTTGTCGAA CTCCAACCGA 
ACCGCTCAAC GGAATCAGCC GGTACAGGAA TTCAATCACG GCCTGGTCCT CAGTCGGGAG 
CCGCTGAGGG ACCGCGATGT CTTCACTGTC CGCATCGACA AGAAGGTGAA CTCTTGGAGC 
GGCTCTATAG AAATCGGTGT GACGGCGCTG GACCCAGCCG GTCTGGAATT TCCCAGCAGC 
GCCACAGGGC TGAAAGGTGG CTCGTGGATT GTGTCTGGCT GCTCGGTGCT GAAGGACGGC 
CGTTCGGTCC TGGAGGAGTA CGGTCGGGAT CTTGACCAGC TTGGGGAAGG AGACCGTGTG 
GGCATCCAGC GGAATGGGAA AGGAGAGCTA CACCTGTGGG TGAATGGGCA GGACTGCGGC 
GCTGCTGCTA GTGGACTTCC ATCTCGACTG TGGGCTGTGG TGGACCTCTA TGGGAAGTGC 
ACACAAGTGA CTGTGGTGTC CTGTGAACCG CCATCTGAAA TGGAGGGTGA CGAGGATGAA 
GAGGTGGAGG ATGAGATTGT GTCGACGCAG GCAGTGTCCA TTATGGCAGA TGCCTGTCGT 
AGTCATGATG ATAGAGCTGC TGTACTGACT GCAGCTGCTG CTACTACAGA GCGGATGAAC 
GGATCAGCGG AGGAAGTGCC TGAAGTGTCA AGGACGCACA GTCGGCCGGA CAAATTTCCA 
AATAACTTTG AGCCTGATAG TGTTTTGACT GAGCATCAGT TGTTTGATGT GTTCAACAAT 
GCCATTGTGT CTCTGTATCG CTCGGAGGAT GAGGGGGCAG AGGATTTGAA TGTTGGGGGT 
GGAGGTGGCG GTTCTGACTC CTCAAGGGGC GGCACAGGAA GTAACGGTGG AGGCAGAGGA 
ACAGGAAGTG ACAGTGGCAT TGGCGGCGGA GGTGGTGGTG GAGGCAGTAG CAGCAATAGC 
AGCCCGGCGG GCAACGCTGG TGGGGCAGCA GGACTAGGGG TGGGCACTGG TGGAATGACG 
ACCAATGATG CACTGCTGTT TCATGAGAAA TGTGGGACGC TGATCAAACT GAGCAACAAT 
AATAAGACAG CAGAAAGAAG GAGACCGCTG GATGAGTTCA ATAATGGAGT CGTCATGACC 
AATCGACCAC TCAGGCACAA CGAAATGTTT GAGATTCGCA TTGACAAACT GGTGGATAAA 
TGGTCTGGTT CCATTGAGAT TGGAGTGACA ACCCACAACC CAAATAATCT TGATTACCCC 
GCAACAATGA CCAATTTGCG CTCAGGCACA ATAATGATGA GTGGCTGTGG GATTTTAACC 
AATGGGAAAG GGACTCGTCG GGAATACTGC GAATTCAGCC TGGATGAACT ACAGGAAGGA 
GATCACATTG GGCTGATGAG GAAAGCCAGT GGAGCACTGC ACTTCTACAT TAATGGCATT 
GATCAAGGGG TTGCAGCCAA CCAGACACCT GGCGTAGTGT ATGGAGTGGT GGATCTCTAT 
GGCATGGCGG TTAAAGTCAC CATTGTGCAC AACCATAACC ACAGTGATCG GTTGCGCCGC 
AATAACGCCA TCATGAGGGC ACTGTCACCT GACGTGGGTC GACCCCGGCC TGCACTCTCA 
TTAACCCCTG AGCCTGATAT CCCAGACCGC TTGCTCTTCC ATCCAAATTG TGGGCAGAAG 
GCTGCAATCA TCAGCGATGG ACGGACTGCT TTACGACCTC ATGCGACGGA TGACTTCAAC 
CATGGTGTTG TGCTTAGCAA TCGTCCATTG CACTCCAATG AGGTTTTCCA GGTGCGCATT 
GACAAAATGG TGGACAAATG GGCTGGCTCC ATAGAGATTG GTGTGACGAC TCATAACCCT 
GCCTATCTTC AGTTACCATC AACTATGACC AACCTGCGCT CGGGAACCTG GATGATGACT 
GGAAATGGGG TCATGCACAA TGGAACTACA ATTCTGGATG AATATGGGCA CAATTTGGAC 
CGTCTAAAAG CTGGAGATAC GGTGGGAGTT GTCCGAAAGG AAGATGGGAG TTTGCATTTC 
TTTGTGAATG GAGTGCCTCA AGGTCCTGCT GCCTGGAATG TTCCGCCCAA CGTGTATGCA 
GTAGTGGATC TGTATGGACA GGCTGCTCAG GCCACCATCA TGGATGATAT GGCTGATCTC 
CCTCCTTTGC CTGACGACAG CTCAGAGGGT CCCACTGCCA TGTCTCCTGG TAGCCCGTGT 
TCAATTTCAG GAGCCACAGC TGCAAATGAC CTGCGCTTTC ATCAGCTGCA TGGCACTAAC 
GCAGTCATCA CCAACGGTGG CCGAACAGCA CTTAGGCAGA ACTGCCGCAG CGAGTTTAAT 
GATGCCATCG TCATTTCAAA CAGGTGCCTT CGAGAAGGAG AGCTCTTTGA AATTGTGATT 
CAGAAAATGG TGGATCGCTG GTCGGGGTCT ATAGAAGCAG GAGTGACAGC GATCAGACCC 
GAAGAACTGG AGTTTCCAAA TACGATGACT GACATTGACT ATGACACATG GATGCTGAGC 
GGCACAGCAA TCATGCAGGA TGGCAATACC ATGAGAAATA ACTATGGCTG TGACTTAGAC 
TCTCTGACTA CTGCCTCCAG GATTGGGATG ATGCGAACAG CAACTGGAGA CCTGCATTAC 
TTCATCAACG GCCTCGACCA GGGTGTAGCT TGTACTGGGC TGCCATCAGA AGTGTATGCT 
GTGGTCGATC TTTATGGTCA GTGTGTGCAA GTGTCCATTA CAAGCTCATC CGGTCCACTG 
GACAACAGTC TGTGTACCAG TAACATCACA GAAAAGAGCT TCCCCATACA CTCCCCGGTG 
GCAGGAGTAG CTCATCGCCT GCACAGTAAA CATGGTAAAA ACGTGGTGTT ATTAGGAGAT 
GGATGCCAAG CGCTGAGGGT CGGAGGTTAT GCCCATGGAA TTGTCTTTAG TGCAAAAGAG 
CTCAAAACAG ATGAAGTGTT TGAGGTGAAA ATTAACAAGG TGGATGAGAG GTGGTCTGGT 
TCTCTTCATG TGGGGCTGAC CACCCTGCAG CCCCCTGACC TGCCATCATG CCCTCTCAGC 
GGCCTCTCGC CTTCACTCAC TCAGCTCAGG ACAAAGGTCA CCTGGGTGCT GGCCGGCTCT 
GAGGTCAGAC GCAACGGAGT ACTGCAGAGA CAGAACTATG GCTGCTCTCT GGATCGCCTC 
ACGGTTGGAA ATCGTGTCGG GGTGAAGAGA TGCAGTGACG ACACCATGCA CATTCTCATT 
GATGGAGAAG ATATGGGACC AGCAGCCACT GCAGTAGCAA AGAACGTGTA TGCTGTATTG 
GACCTGTATG GAAAGGTGAC GGCTGTGTCC ATCGTCAGTT CAACACTAGT TGAGGATTCT 
GAGAGTGTGA AAGCTCCCTC GCTCTCGTCC GACAGCTGCA GTGAAGGAGA AGAGGACTGC 
ACTCCTGTCA GAGAGTGTGA GAATGAGCCT CCATTGGTGC CCACAGTCAT GACGTTTTTG 
GAGAATCATG GGAAGAATAT CCAGCTGTCC AATCAAAATC TGACCGCCGC TCGGGTGTCC 
AGCTACAACC AGGGGCTTCT GGTCACAGCC CAAGCTCTGC CTCGCCAGCA GCTCTTCCAG 
TTTCAGATTG ACCGCTTGAA TCCCTCATGG ACGTCGTCTC TCTCCCTCGG TGTGATTGGT 
CATTCACCAG ACCGACTCAA CTTTCCTTCC ACAGCCTGCT GCCTTAAACG CTCAGTCTGG 
CTACTGCAAC GAGACTCTGT TTTCCACAAC TCGCTAAAGA TTTGTGAGAA CTATGGTCCA 
AACCTGGACA CCTGTCCAGA GGGGACAGTT TTGGGGCTGT TGGTGGATAG CAGTGGTTGT 
CTCCATTTGT TTGTGAATGG CATGGATCAG GGTGTAGCGG CACAGGACAT CCCGAGCCCC 
TGTTACGCCC TCATTGACCT TTACGGCCAG TGTGAACAGG TGACTATCGT GGCGAATCAT 
GTGCCGGTGG TTGGCGGTGA GCGAGGAGAC CTGCGCTGTC AAGGGGACAT GGAGAAGGCT 
GATATGGTGG ACGGTATAAA AGAGAGTGTC TGCTGGACTC CTCCTCCCGA GGTCAACCCT 
AATAAGACCT GCGAGTATCA GGCGCTGTGC TCCAGATTCA AAGACCTGCT CACACTGCCT 
GATGGTTACT TTAATGAGGA TGCCAAGTAT AACCTGTGTT ACTGTGAGTC CTGTCATAAA 
TTAAGGGGTG ATGAGGCCTA TTACAAGCGA GGAGAGCCCC CCAGAGACTA TGCTCTACCG 
TTCGGCTGGT GCCGCTTTGC ACTCCAGATC AAGACCCACT GTGAAGTCTC CAATGCCTTC 
AAGAAGTGGC ACATTGCGTA TCACGGCACA AGTGTCGGTT CTCTTAGACG CACCCTGGAC 
CACAACCAAC TGTTATCAGC AGAATCATCC TCTATTTTCT CGTCATCGTC GGTGAAGACG 
GAGGGTCATG GCAGCTACGG AGAGCCGGAG GAGAACAGCG CTCCAGAGAG AGACATTCCC 
AGAGTGCAGC TCTCACCAAC CATGCGATAT TCCGGCCTCG AGGTCTTCGC TCCGAAAGTG 
CAATTTCGAG ACCCGCGTTC GCTCCGCTGT CACCAGGCTC AGGTGGGCTT CCAGGTGTGT 
GTCCGGCCAG GCTCCTATAA AGTAGGTCCG TCGTCTTTAG GAATCAGCGA GCCGCTAGAC 
CCGCGATTCA GCAATGCAGA AATCGAATGG ATCACCAAGG AGAAAGGAGG CACGCTTCTA 
TATGGCCTGC TCATACGAGT TGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

neurl4 ( XM_677822.9 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_682914.5 Danio rerio neuralized E3 ubiquitin protein ligase 4 (neurl4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa17890
Clone ID Related Accession (Same CDS sequence)	XM_677822.8 , XM_677822.9 , XM_021480310.1
Accession Version	XM_677822.9 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neuralized-like protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394839.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_677822.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG AGCTGCATCC GCGCAGCGGA AAACTGATCG GTTTGTCGAA CTCCAACCGA 
ACCGCTCAAC GGAATCAGCC GGTACAGGAA TTCAATCACG GCCTGGTCCT CAGTCGGGAG 
CCGCTGAGGG ACCGCGATGT CTTCACTGTC CGCATCGACA AGAAGGTGAA CTCTTGGAGC 
GGCTCTATAG AAATCGGTGT GACGGCGCTG GACCCAGCCG GTCTGGAATT TCCCAGCAGC 
GCCACAGGGC TGAAAGGTGG CTCGTGGATT GTGTCTGGCT GCTCGGTGCT GAAGGACGGC 
CGTTCGGTCC TGGAGGAGTA CGGTCGGGAT CTTGACCAGC TTGGGGAAGG AGACCGTGTG 
GGCATCCAGC GGAATGGGAA AGGAGAGCTA CACCTGTGGG TGAATGGGCA GGACTGCGGC 
GCTGCTGCTA GTGGACTTCC ATCTCGACTG TGGGCTGTGG TGGACCTCTA TGGGAAGTGC 
ACACAAGTGA CTGTGGTGTC CTGTGAACCG CCATCTGAAA TGGAGGGTGA CGAGGATGAA 
GAGGTGGAGG ATGAGATTGT GTCGACGCAG GCAGTGTCCA TTATGGCAGA TGCCTGTCGT 
AGTCATGATG ATAGAGCTGC TGTACTGACT GCAGCTGCTG CTACTACAGA GCGGATGAAC 
GGATCAGCGG AGGAAGTGCC TGAAGTGTCA AGGACGCACA GTCGGCCGGA CAAATTTCCA 
AATAACTTTG AGCCTGATAG TGTTTTGACT GAGCATCAGT TGTTTGATGT GTTCAACAAT 
GCCATTGTGT CTCTGTATCG CTCGGAGGAT GAGGGGGCAG AGGATTTGAA TGTTGGGGGT 
GGAGGTGGCG GTTCTGACTC CTCAAGGGGC GGCACAGGAA GTAACGGTGG AGGCAGAGGA 
ACAGGAAGTG ACAGTGGCAT TGGCGGCGGA GGTGGTGGTG GAGGCAGTAG CAGCAATAGC 
AGCCCGGCGG GCAACGCTGG TGGGGCAGCA GGACTAGGGG TGGGCACTGG TGGAATGACG 
ACCAATGATG CACTGCTGTT TCATGAGAAA TGTGGGACGC TGATCAAACT GAGCAACAAT 
AATAAGACAG CAGAAAGAAG GAGACCGCTG GATGAGTTCA ATAATGGAGT CGTCATGACC 
AATCGACCAC TCAGGCACAA CGAAATGTTT GAGATTCGCA TTGACAAACT GGTGGATAAA 
TGGTCTGGTT CCATTGAGAT TGGAGTGACA ACCCACAACC CAAATAATCT TGATTACCCC 
GCAACAATGA CCAATTTGCG CTCAGGCACA ATAATGATGA GTGGCTGTGG GATTTTAACC 
AATGGGAAAG GGACTCGTCG GGAATACTGC GAATTCAGCC TGGATGAACT ACAGGAAGGA 
GATCACATTG GGCTGATGAG GAAAGCCAGT GGAGCACTGC ACTTCTACAT TAATGGCATT 
GATCAAGGGG TTGCAGCCAA CCAGACACCT GGCGTAGTGT ATGGAGTGGT GGATCTCTAT 
GGCATGGCGG TTAAAGTCAC CATTGTGCAC AACCATAACC ACAGTGATCG GTTGCGCCGC 
AATAACGCCA TCATGAGGGC ACTGTCACCT GACGTGGGTC GACCCCGGCC TGCACTCTCA 
TTAACCCCTG AGCCTGATAT CCCAGACCGC TTGCTCTTCC ATCCAAATTG TGGGCAGAAG 
GCTGCAATCA TCAGCGATGG ACGGACTGCT TTACGACCTC ATGCGACGGA TGACTTCAAC 
CATGGTGTTG TGCTTAGCAA TCGTCCATTG CACTCCAATG AGGTTTTCCA GGTGCGCATT 
GACAAAATGG TGGACAAATG GGCTGGCTCC ATAGAGATTG GTGTGACGAC TCATAACCCT 
GCCTATCTTC AGTTACCATC AACTATGACC AACCTGCGCT CGGGAACCTG GATGATGACT 
GGAAATGGGG TCATGCACAA TGGAACTACA ATTCTGGATG AATATGGGCA CAATTTGGAC 
CGTCTAAAAG CTGGAGATAC GGTGGGAGTT GTCCGAAAGG AAGATGGGAG TTTGCATTTC 
TTTGTGAATG GAGTGCCTCA AGGTCCTGCT GCCTGGAATG TTCCGCCCAA CGTGTATGCA 
GTAGTGGATC TGTATGGACA GGCTGCTCAG GCCACCATCA TGGATGATAT GGCTGATCTC 
CCTCCTTTGC CTGACGACAG CTCAGAGGGT CCCACTGCCA TGTCTCCTGG TAGCCCGTGT 
TCAATTTCAG GAGCCACAGC TGCAAATGAC CTGCGCTTTC ATCAGCTGCA TGGCACTAAC 
GCAGTCATCA CCAACGGTGG CCGAACAGCA CTTAGGCAGA ACTGCCGCAG CGAGTTTAAT 
GATGCCATCG TCATTTCAAA CAGGTGCCTT CGAGAAGGAG AGCTCTTTGA AATTGTGATT 
CAGAAAATGG TGGATCGCTG GTCGGGGTCT ATAGAAGCAG GAGTGACAGC GATCAGACCC 
GAAGAACTGG AGTTTCCAAA TACGATGACT GACATTGACT ATGACACATG GATGCTGAGC 
GGCACAGCAA TCATGCAGGA TGGCAATACC ATGAGAAATA ACTATGGCTG TGACTTAGAC 
TCTCTGACTA CTGCCTCCAG GATTGGGATG ATGCGAACAG CAACTGGAGA CCTGCATTAC 
TTCATCAACG GCCTCGACCA GGGTGTAGCT TGTACTGGGC TGCCATCAGA AGTGTATGCT 
GTGGTCGATC TTTATGGTCA GTGTGTGCAA GTGTCCATTA CAAGCTCATC CGGTCCACTG 
GACAACAGTC TGTGTACCAG TAACATCACA GAAAAGAGCT TCCCCATACA CTCCCCGGTG 
GCAGGAGTAG CTCATCGCCT GCACAGTAAA CATGGTAAAA ACGTGGTGTT ATTAGGAGAT 
GGATGCCAAG CGCTGAGGGT CGGAGGTTAT GCCCATGGAA TTGTCTTTAG TGCAAAAGAG 
CTCAAAACAG ATGAAGTGTT TGAGGTGAAA ATTAACAAGG TGGATGAGAG GTGGTCTGGT 
TCTCTTCATG TGGGGCTGAC CACCCTGCAG CCCCCTGACC TGCCATCATG CCCTCTCAGC 
GGCCTCTCGC CTTCACTCAC TCAGCTCAGG ACAAAGGTCA CCTGGGTGCT GGCCGGCTCT 
GAGGTCAGAC GCAACGGAGT ACTGCAGAGA CAGAACTATG GCTGCTCTCT GGATCGCCTC 
ACGGTTGGAA ATCGTGTCGG GGTGAAGAGA TGCAGTGACG ACACCATGCA CATTCTCATT 
GATGGAGAAG ATATGGGACC AGCAGCCACT GCAGTAGCAA AGAACGTGTA TGCTGTATTG 
GACCTGTATG GAAAGGTGAC GGCTGTGTCC ATCGTCAGTT CAACACTAGT TGAGGATTCT 
GAGAGTGTGA AAGCTCCCTC GCTCTCGTCC GACAGCTGCA GTGAAGGAGA AGAGGACTGC 
ACTCCTGTCA GAGAGTGTGA GAATGAGCCT CCATTGGTGC CCACAGTCAT GACGTTTTTG 
GAGAATCATG GGAAGAATAT CCAGCTGTCC AATCAAAATC TGACCGCCGC TCGGGTGTCC 
AGCTACAACC AGGGGCTTCT GGTCACAGCC CAAGCTCTGC CTCGCCAGCA GCTCTTCCAG 
TTTCAGATTG ACCGCTTGAA TCCCTCATGG ACGTCGTCTC TCTCCCTCGG TGTGATTGGT 
CATTCACCAG ACCGACTCAA CTTTCCTTCC ACAGCCTGCT GCCTTAAACG CTCAGTCTGG 
CTACTGCAAC GAGACTCTGT TTTCCACAAC TCGCTAAAGA TTTGTGAGAA CTATGGTCCA 
AACCTGGACA CCTGTCCAGA GGGGACAGTT TTGGGGCTGT TGGTGGATAG CAGTGGTTGT 
CTCCATTTGT TTGTGAATGG CATGGATCAG GGTGTAGCGG CACAGGACAT CCCGAGCCCC 
TGTTACGCCC TCATTGACCT TTACGGCCAG TGTGAACAGG TGACTATCGT GGCGAATCAT 
GTGCCGGTGG TTGGCGGTGA GCGAGGAGAC CTGCGCTGTC AAGGGGACAT GGAGAAGGCT 
GATATGGTGG ACGGTATAAA AGAGAGTGTC TGCTGGACTC CTCCTCCCGA GGTCAACCCT 
AATAAGACCT GCGAGTATCA GGCGCTGTGC TCCAGATTCA AAGACCTGCT CACACTGCCT 
GATGGTTACT TTAATGAGGA TGCCAAGTAT AACCTGTGTT ACTGTGAGTC CTGTCATAAA 
TTAAGGGGTG ATGAGGCCTA TTACAAGCGA GGAGAGCCCC CCAGAGACTA TGCTCTACCG 
TTCGGCTGGT GCCGCTTTGC ACTCCAGATC AAGACCCACT GTGAAGTCTC CAATGCCTTC 
AAGAAGTGGC ACATTGCGTA TCACGGCACA AGTGTCGGTT CTCTTAGACG CACCCTGGAC 
CACAACCAAC TGTTATCAGC AGAATCATCC TCTATTTTCT CGTCATCGTC GGTGAAGACG 
GAGGGTCATG GCAGCTACGG AGAGCCGGAG GAGAACAGCG CTCCAGAGAG AGACATTCCC 
AGAGTGCAGC TCTCACCAAC CATGCGATAT TCCGGCCTCG AGGTCTTCGC TCCGAAAGTG 
CAATTTCGAG ACCCGCGTTC GCTCCGCTGT CACCAGGCTC AGGTGGGCTT CCAGGTGTGT 
GTCCGGCCAG GCTCCTATAA AGTAGGTCCG TCGTCTTTAG GAATCAGCGA GCCGCTAGAC 
CCGCGATTCA GCAATGCAGA AATCGAATGG ATCACCAAGG AGAAAGGAGG CACGCTTCTA 
TATGGCCTGC TCATACGAGT TGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_682914.5
CDS	317..5083
Translation	MAAELHPRSGKLIGLSNSNRTAQRNQPVQEFNHGLVLSREPLRDRDVFTVRIDKKVNSWSGSIEIGVTALDPAGLEFPSSATGLKGGSWIVSGCSVLKDGRSVLEEYGRDLDQLGEGDRVGIQRNGKGELHLWVNGQDCGAAASGLPSRLWAVVDLYGKCTQVTVVSCEPPSEMEGDEDEEVEDEIVSTQAVSIMADACRSHDDRAAVLTAAAATTERMNGSAEEVPEVSRTHSRPDKFPNNFEPDSVLTEHQLFDVFNNAIVSLYRSEDEGAEDLNVGGGGGGSDSSRGGTGSNGGGRGTGSDSGIGGGGGGGGSSSNSSPAGNAGGAAGLGVGTGGMTTNDALLFHEKCGTLIKLSNNNKTAERRRPLDEFNNGVVMTNRPLRHNEMFEIRIDKLVDKWSGSIEIGVTTHNPNNLDYPATMTNLRSGTIMMSGCGILTNGKGTRREYCEFSLDELQEGDHIGLMRKASGALHFYINGIDQGVAANQTPGVVYGVVDLYGMAVKVTIVHNHNHSDRLRRNNAIMRALSPDVGRPRPALSLTPEPDIPDRLLFHPNCGQKAAIISDGRTALRPHATDDFNHGVVLSNRPLHSNEVFQVRIDKMVDKWAGSIEIGVTTHNPAYLQLPSTMTNLRSGTWMMTGNGVMHNGTTILDEYGHNLDRLKAGDTVGVVRKEDGSLHFFVNGVPQGPAAWNVPPNVYAVVDLYGQAAQATIMDDMADLPPLPDDSSEGPTAMSPGSPCSISGATAANDLRFHQLHGTNAVITNGGRTALRQNCRSEFNDAIVISNRCLREGELFEIVIQKMVDRWSGSIEAGVTAIRPEELEFPNTMTDIDYDTWMLSGTAIMQDGNTMRNNYGCDLDSLTTASRIGMMRTATGDLHYFINGLDQGVACTGLPSEVYAVVDLYGQCVQVSITSSSGPLDNSLCTSNITEKSFPIHSPVAGVAHRLHSKHGKNVVLLGDGCQALRVGGYAHGIVFSAKELKTDEVFEVKINKVDERWSGSLHVGLTTLQPPDLPSCPLSGLSPSLTQLRTKVTWVLAGSEVRRNGVLQRQNYGCSLDRLTVGNRVGVKRCSDDTMHILIDGEDMGPAATAVAKNVYAVLDLYGKVTAVSIVSSTLVEDSESVKAPSLSSDSCSEGEEDCTPVRECENEPPLVPTVMTFLENHGKNIQLSNQNLTAARVSSYNQGLLVTAQALPRQQLFQFQIDRLNPSWTSSLSLGVIGHSPDRLNFPSTACCLKRSVWLLQRDSVFHNSLKICENYGPNLDTCPEGTVLGLLVDSSGCLHLFVNGMDQGVAAQDIPSPCYALIDLYGQCEQVTIVANHVPVVGGERGDLRCQGDMEKADMVDGIKESVCWTPPPEVNPNKTCEYQALCSRFKDLLTLPDGYFNEDAKYNLCYCESCHKLRGDEAYYKRGEPPRDYALPFGWCRFALQIKTHCEVSNAFKKWHIAYHGTSVGSLRRTLDHNQLLSAESSSIFSSSSVKTEGHGSYGEPEENSAPERDIPRVQLSPTMRYSGLEVFAPKVQFRDPRSLRCHQAQVGFQVCVRPGSYKVGPSSLGISEPLDPRFSNAEIEWITKEKGGTLLYGLLIRVE

Target ORF information:

RefSeq Version	XM_677822.9
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neuralized E3 ubiquitin protein ligase 4 (neurl4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_677822.9

ORF Insert Sequence:

ATGGCGGCTG AGCTGCATCC GCGCAGCGGA AAACTGATCG GTTTGTCGAA CTCCAACCGA 
ACCGCTCAAC GGAATCAGCC GGTACAGGAA TTCAATCACG GCCTGGTCCT CAGTCGGGAG 
CCGCTGAGGG ACCGCGATGT CTTCACTGTC CGCATCGACA AGAAGGTGAA CTCTTGGAGC 
GGCTCTATAG AAATCGGTGT GACGGCGCTG GACCCAGCCG GTCTGGAATT TCCCAGCAGC 
GCCACAGGGC TGAAAGGTGG CTCGTGGATT GTGTCTGGCT GCTCGGTGCT GAAGGACGGC 
CGTTCGGTCC TGGAGGAGTA CGGTCGGGAT CTTGACCAGC TTGGGGAAGG AGACCGTGTG 
GGCATCCAGC GGAATGGGAA AGGAGAGCTA CACCTGTGGG TGAATGGGCA GGACTGCGGC 
GCTGCTGCTA GTGGACTTCC ATCTCGACTG TGGGCTGTGG TGGACCTCTA TGGGAAGTGC 
ACACAAGTGA CTGTGGTGTC CTGTGAACCG CCATCTGAAA TGGAGGGTGA CGAGGATGAA 
GAGGTGGAGG ATGAGATTGT GTCGACGCAG GCAGTGTCCA TTATGGCAGA TGCCTGTCGT 
AGTCATGATG ATAGAGCTGC TGTACTGACT GCAGCTGCTG CTACTACAGA GCGGATGAAC 
GGATCAGCGG AGGAAGTGCC TGAAGTGTCA AGGACGCACA GTCGGCCGGA CAAATTTCCA 
AATAACTTTG AGCCTGATAG TGTTTTGACT GAGCATCAGT TGTTTGATGT GTTCAACAAT 
GCCATTGTGT CTCTGTATCG CTCGGAGGAT GAGGGGGCAG AGGATTTGAA TGTTGGGGGT 
GGAGGTGGCG GTTCTGACTC CTCAAGGGGC GGCACAGGAA GTAACGGTGG AGGCAGAGGA 
ACAGGAAGTG ACAGTGGCAT TGGCGGCGGA GGTGGTGGTG GAGGCAGTAG CAGCAATAGC 
AGCCCGGCGG GCAACGCTGG TGGGGCAGCA GGACTAGGGG TGGGCACTGG TGGAATGACG 
ACCAATGATG CACTGCTGTT TCATGAGAAA TGTGGGACGC TGATCAAACT GAGCAACAAT 
AATAAGACAG CAGAAAGAAG GAGACCGCTG GATGAGTTCA ATAATGGAGT CGTCATGACC 
AATCGACCAC TCAGGCACAA CGAAATGTTT GAGATTCGCA TTGACAAACT GGTGGATAAA 
TGGTCTGGTT CCATTGAGAT TGGAGTGACA ACCCACAACC CAAATAATCT TGATTACCCC 
GCAACAATGA CCAATTTGCG CTCAGGCACA ATAATGATGA GTGGCTGTGG GATTTTAACC 
AATGGGAAAG GGACTCGTCG GGAATACTGC GAATTCAGCC TGGATGAACT ACAGGAAGGA 
GATCACATTG GGCTGATGAG GAAAGCCAGT GGAGCACTGC ACTTCTACAT TAATGGCATT 
GATCAAGGGG TTGCAGCCAA CCAGACACCT GGCGTAGTGT ATGGAGTGGT GGATCTCTAT 
GGCATGGCGG TTAAAGTCAC CATTGTGCAC AACCATAACC ACAGTGATCG GTTGCGCCGC 
AATAACGCCA TCATGAGGGC ACTGTCACCT GACGTGGGTC GACCCCGGCC TGCACTCTCA 
TTAACCCCTG AGCCTGATAT CCCAGACCGC TTGCTCTTCC ATCCAAATTG TGGGCAGAAG 
GCTGCAATCA TCAGCGATGG ACGGACTGCT TTACGACCTC ATGCGACGGA TGACTTCAAC 
CATGGTGTTG TGCTTAGCAA TCGTCCATTG CACTCCAATG AGGTTTTCCA GGTGCGCATT 
GACAAAATGG TGGACAAATG GGCTGGCTCC ATAGAGATTG GTGTGACGAC TCATAACCCT 
GCCTATCTTC AGTTACCATC AACTATGACC AACCTGCGCT CGGGAACCTG GATGATGACT 
GGAAATGGGG TCATGCACAA TGGAACTACA ATTCTGGATG AATATGGGCA CAATTTGGAC 
CGTCTAAAAG CTGGAGATAC GGTGGGAGTT GTCCGAAAGG AAGATGGGAG TTTGCATTTC 
TTTGTGAATG GAGTGCCTCA AGGTCCTGCT GCCTGGAATG TTCCGCCCAA CGTGTATGCA 
GTAGTGGATC TGTATGGACA GGCTGCTCAG GCCACCATCA TGGATGATAT GGCTGATCTC 
CCTCCTTTGC CTGACGACAG CTCAGAGGGT CCCACTGCCA TGTCTCCTGG TAGCCCGTGT 
TCAATTTCAG GAGCCACAGC TGCAAATGAC CTGCGCTTTC ATCAGCTGCA TGGCACTAAC 
GCAGTCATCA CCAACGGTGG CCGAACAGCA CTTAGGCAGA ACTGCCGCAG CGAGTTTAAT 
GATGCCATCG TCATTTCAAA CAGGTGCCTT CGAGAAGGAG AGCTCTTTGA AATTGTGATT 
CAGAAAATGG TGGATCGCTG GTCGGGGTCT ATAGAAGCAG GAGTGACAGC GATCAGACCC 
GAAGAACTGG AGTTTCCAAA TACGATGACT GACATTGACT ATGACACATG GATGCTGAGC 
GGCACAGCAA TCATGCAGGA TGGCAATACC ATGAGAAATA ACTATGGCTG TGACTTAGAC 
TCTCTGACTA CTGCCTCCAG GATTGGGATG ATGCGAACAG CAACTGGAGA CCTGCATTAC 
TTCATCAACG GCCTCGACCA GGGTGTAGCT TGTACTGGGC TGCCATCAGA AGTGTATGCT 
GTGGTCGATC TTTATGGTCA GTGTGTGCAA GTGTCCATTA CAAGCTCATC CGGTCCACTG 
GACAACAGTC TGTGTACCAG TAACATCACA GAAAAGAGCT TCCCCATACA CTCCCCGGTG 
GCAGGAGTAG CTCATCGCCT GCACAGTAAA CATGGTAAAA ACGTGGTGTT ATTAGGAGAT 
GGATGCCAAG CGCTGAGGGT CGGAGGTTAT GCCCATGGAA TTGTCTTTAG TGCAAAAGAG 
CTCAAAACAG ATGAAGTGTT TGAGGTGAAA ATTAACAAGG TGGATGAGAG GTGGTCTGGT 
TCTCTTCATG TGGGGCTGAC CACCCTGCAG CCCCCTGACC TGCCATCATG CCCTCTCAGC 
GGCCTCTCGC CTTCACTCAC TCAGCTCAGG ACAAAGGTCA CCTGGGTGCT GGCCGGCTCT 
GAGGTCAGAC GCAACGGAGT ACTGCAGAGA CAGAACTATG GCTGCTCTCT GGATCGCCTC 
ACGGTTGGAA ATCGTGTCGG GGTGAAGAGA TGCAGTGACG ACACCATGCA CATTCTCATT 
GATGGAGAAG ATATGGGACC AGCAGCCACT GCAGTAGCAA AGAACGTGTA TGCTGTATTG 
GACCTGTATG GAAAGGTGAC GGCTGTGTCC ATCGTCAGTT CAACACTAGT TGAGGATTCT 
GAGAGTGTGA AAGCTCCCTC GCTCTCGTCC GACAGCTGCA GTGAAGGAGA AGAGGACTGC 
ACTCCTGTCA GAGAGTGTGA GAATGAGCCT CCATTGGTGC CCACAGTCAT GACGTTTTTG 
GAGAATCATG GGAAGAATAT CCAGCTGTCC AATCAAAATC TGACCGCCGC TCGGGTGTCC 
AGCTACAACC AGGGGCTTCT GGTCACAGCC CAAGCTCTGC CTCGCCAGCA GCTCTTCCAG 
TTTCAGATTG ACCGCTTGAA TCCCTCATGG ACGTCGTCTC TCTCCCTCGG TGTGATTGGT 
CATTCACCAG ACCGACTCAA CTTTCCTTCC ACAGCCTGCT GCCTTAAACG CTCAGTCTGG 
CTACTGCAAC GAGACTCTGT TTTCCACAAC TCGCTAAAGA TTTGTGAGAA CTATGGTCCA 
AACCTGGACA CCTGTCCAGA GGGGACAGTT TTGGGGCTGT TGGTGGATAG CAGTGGTTGT 
CTCCATTTGT TTGTGAATGG CATGGATCAG GGTGTAGCGG CACAGGACAT CCCGAGCCCC 
TGTTACGCCC TCATTGACCT TTACGGCCAG TGTGAACAGG TGACTATCGT GGCGAATCAT 
GTGCCGGTGG TTGGCGGTGA GCGAGGAGAC CTGCGCTGTC AAGGGGACAT GGAGAAGGCT 
GATATGGTGG ACGGTATAAA AGAGAGTGTC TGCTGGACTC CTCCTCCCGA GGTCAACCCT 
AATAAGACCT GCGAGTATCA GGCGCTGTGC TCCAGATTCA AAGACCTGCT CACACTGCCT 
GATGGTTACT TTAATGAGGA TGCCAAGTAT AACCTGTGTT ACTGTGAGTC CTGTCATAAA 
TTAAGGGGTG ATGAGGCCTA TTACAAGCGA GGAGAGCCCC CCAGAGACTA TGCTCTACCG 
TTCGGCTGGT GCCGCTTTGC ACTCCAGATC AAGACCCACT GTGAAGTCTC CAATGCCTTC 
AAGAAGTGGC ACATTGCGTA TCACGGCACA AGTGTCGGTT CTCTTAGACG CACCCTGGAC 
CACAACCAAC TGTTATCAGC AGAATCATCC TCTATTTTCT CGTCATCGTC GGTGAAGACG 
GAGGGTCATG GCAGCTACGG AGAGCCGGAG GAGAACAGCG CTCCAGAGAG AGACATTCCC 
AGAGTGCAGC TCTCACCAAC CATGCGATAT TCCGGCCTCG AGGTCTTCGC TCCGAAAGTG 
CAATTTCGAG ACCCGCGTTC GCTCCGCTGT CACCAGGCTC AGGTGGGCTT CCAGGTGTGT 
GTCCGGCCAG GCTCCTATAA AGTAGGTCCG TCGTCTTTAG GAATCAGCGA GCCGCTAGAC 
CCGCGATTCA GCAATGCAGA AATCGAATGG ATCACCAAGG AGAAAGGAGG CACGCTTCTA 
TATGGCCTGC TCATACGAGT TGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

neurl4 ( XM_021480310.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335985.1 Danio rerio neuralized E3 ubiquitin protein ligase 4 (neurl4), transcript variant X1, mRNA.

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CloneID	ODa17890
Clone ID Related Accession (Same CDS sequence)	XM_677822.8 , XM_677822.9 , XM_021480310.1
Accession Version	XM_021480310.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neuralized-like protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG AGCTGCATCC GCGCAGCGGA AAACTGATCG GTTTGTCGAA CTCCAACCGA 
ACCGCTCAAC GGAATCAGCC GGTACAGGAA TTCAATCACG GCCTGGTCCT CAGTCGGGAG 
CCGCTGAGGG ACCGCGATGT CTTCACTGTC CGCATCGACA AGAAGGTGAA CTCTTGGAGC 
GGCTCTATAG AAATCGGTGT GACGGCGCTG GACCCAGCCG GTCTGGAATT TCCCAGCAGC 
GCCACAGGGC TGAAAGGTGG CTCGTGGATT GTGTCTGGCT GCTCGGTGCT GAAGGACGGC 
CGTTCGGTCC TGGAGGAGTA CGGTCGGGAT CTTGACCAGC TTGGGGAAGG AGACCGTGTG 
GGCATCCAGC GGAATGGGAA AGGAGAGCTA CACCTGTGGG TGAATGGGCA GGACTGCGGC 
GCTGCTGCTA GTGGACTTCC ATCTCGACTG TGGGCTGTGG TGGACCTCTA TGGGAAGTGC 
ACACAAGTGA CTGTGGTGTC CTGTGAACCG CCATCTGAAA TGGAGGGTGA CGAGGATGAA 
GAGGTGGAGG ATGAGATTGT GTCGACGCAG GCAGTGTCCA TTATGGCAGA TGCCTGTCGT 
AGTCATGATG ATAGAGCTGC TGTACTGACT GCAGCTGCTG CTACTACAGA GCGGATGAAC 
GGATCAGCGG AGGAAGTGCC TGAAGTGTCA AGGACGCACA GTCGGCCGGA CAAATTTCCA 
AATAACTTTG AGCCTGATAG TGTTTTGACT GAGCATCAGT TGTTTGATGT GTTCAACAAT 
GCCATTGTGT CTCTGTATCG CTCGGAGGAT GAGGGGGCAG AGGATTTGAA TGTTGGGGGT 
GGAGGTGGCG GTTCTGACTC CTCAAGGGGC GGCACAGGAA GTAACGGTGG AGGCAGAGGA 
ACAGGAAGTG ACAGTGGCAT TGGCGGCGGA GGTGGTGGTG GAGGCAGTAG CAGCAATAGC 
AGCCCGGCGG GCAACGCTGG TGGGGCAGCA GGACTAGGGG TGGGCACTGG TGGAATGACG 
ACCAATGATG CACTGCTGTT TCATGAGAAA TGTGGGACGC TGATCAAACT GAGCAACAAT 
AATAAGACAG CAGAAAGAAG GAGACCGCTG GATGAGTTCA ATAATGGAGT CGTCATGACC 
AATCGACCAC TCAGGCACAA CGAAATGTTT GAGATTCGCA TTGACAAACT GGTGGATAAA 
TGGTCTGGTT CCATTGAGAT TGGAGTGACA ACCCACAACC CAAATAATCT TGATTACCCC 
GCAACAATGA CCAATTTGCG CTCAGGCACA ATAATGATGA GTGGCTGTGG GATTTTAACC 
AATGGGAAAG GGACTCGTCG GGAATACTGC GAATTCAGCC TGGATGAACT ACAGGAAGGA 
GATCACATTG GGCTGATGAG GAAAGCCAGT GGAGCACTGC ACTTCTACAT TAATGGCATT 
GATCAAGGGG TTGCAGCCAA CCAGACACCT GGCGTAGTGT ATGGAGTGGT GGATCTCTAT 
GGCATGGCGG TTAAAGTCAC CATTGTGCAC AACCATAACC ACAGTGATCG GTTGCGCCGC 
AATAACGCCA TCATGAGGGC ACTGTCACCT GACGTGGGTC GACCCCGGCC TGCACTCTCA 
TTAACCCCTG AGCCTGATAT CCCAGACCGC TTGCTCTTCC ATCCAAATTG TGGGCAGAAG 
GCTGCAATCA TCAGCGATGG ACGGACTGCT TTACGACCTC ATGCGACGGA TGACTTCAAC 
CATGGTGTTG TGCTTAGCAA TCGTCCATTG CACTCCAATG AGGTTTTCCA GGTGCGCATT 
GACAAAATGG TGGACAAATG GGCTGGCTCC ATAGAGATTG GTGTGACGAC TCATAACCCT 
GCCTATCTTC AGTTACCATC AACTATGACC AACCTGCGCT CGGGAACCTG GATGATGACT 
GGAAATGGGG TCATGCACAA TGGAACTACA ATTCTGGATG AATATGGGCA CAATTTGGAC 
CGTCTAAAAG CTGGAGATAC GGTGGGAGTT GTCCGAAAGG AAGATGGGAG TTTGCATTTC 
TTTGTGAATG GAGTGCCTCA AGGTCCTGCT GCCTGGAATG TTCCGCCCAA CGTGTATGCA 
GTAGTGGATC TGTATGGACA GGCTGCTCAG GCCACCATCA TGGATGATAT GGCTGATCTC 
CCTCCTTTGC CTGACGACAG CTCAGAGGGT CCCACTGCCA TGTCTCCTGG TAGCCCGTGT 
TCAATTTCAG GAGCCACAGC TGCAAATGAC CTGCGCTTTC ATCAGCTGCA TGGCACTAAC 
GCAGTCATCA CCAACGGTGG CCGAACAGCA CTTAGGCAGA ACTGCCGCAG CGAGTTTAAT 
GATGCCATCG TCATTTCAAA CAGGTGCCTT CGAGAAGGAG AGCTCTTTGA AATTGTGATT 
CAGAAAATGG TGGATCGCTG GTCGGGGTCT ATAGAAGCAG GAGTGACAGC GATCAGACCC 
GAAGAACTGG AGTTTCCAAA TACGATGACT GACATTGACT ATGACACATG GATGCTGAGC 
GGCACAGCAA TCATGCAGGA TGGCAATACC ATGAGAAATA ACTATGGCTG TGACTTAGAC 
TCTCTGACTA CTGCCTCCAG GATTGGGATG ATGCGAACAG CAACTGGAGA CCTGCATTAC 
TTCATCAACG GCCTCGACCA GGGTGTAGCT TGTACTGGGC TGCCATCAGA AGTGTATGCT 
GTGGTCGATC TTTATGGTCA GTGTGTGCAA GTGTCCATTA CAAGCTCATC CGGTCCACTG 
GACAACAGTC TGTGTACCAG TAACATCACA GAAAAGAGCT TCCCCATACA CTCCCCGGTG 
GCAGGAGTAG CTCATCGCCT GCACAGTAAA CATGGTAAAA ACGTGGTGTT ATTAGGAGAT 
GGATGCCAAG CGCTGAGGGT CGGAGGTTAT GCCCATGGAA TTGTCTTTAG TGCAAAAGAG 
CTCAAAACAG ATGAAGTGTT TGAGGTGAAA ATTAACAAGG TGGATGAGAG GTGGTCTGGT 
TCTCTTCATG TGGGGCTGAC CACCCTGCAG CCCCCTGACC TGCCATCATG CCCTCTCAGC 
GGCCTCTCGC CTTCACTCAC TCAGCTCAGG ACAAAGGTCA CCTGGGTGCT GGCCGGCTCT 
GAGGTCAGAC GCAACGGAGT ACTGCAGAGA CAGAACTATG GCTGCTCTCT GGATCGCCTC 
ACGGTTGGAA ATCGTGTCGG GGTGAAGAGA TGCAGTGACG ACACCATGCA CATTCTCATT 
GATGGAGAAG ATATGGGACC AGCAGCCACT GCAGTAGCAA AGAACGTGTA TGCTGTATTG 
GACCTGTATG GAAAGGTGAC GGCTGTGTCC ATCGTCAGTT CAACACTAGT TGAGGATTCT 
GAGAGTGTGA AAGCTCCCTC GCTCTCGTCC GACAGCTGCA GTGAAGGAGA AGAGGACTGC 
ACTCCTGTCA GAGAGTGTGA GAATGAGCCT CCATTGGTGC CCACAGTCAT GACGTTTTTG 
GAGAATCATG GGAAGAATAT CCAGCTGTCC AATCAAAATC TGACCGCCGC TCGGGTGTCC 
AGCTACAACC AGGGGCTTCT GGTCACAGCC CAAGCTCTGC CTCGCCAGCA GCTCTTCCAG 
TTTCAGATTG ACCGCTTGAA TCCCTCATGG ACGTCGTCTC TCTCCCTCGG TGTGATTGGT 
CATTCACCAG ACCGACTCAA CTTTCCTTCC ACAGCCTGCT GCCTTAAACG CTCAGTCTGG 
CTACTGCAAC GAGACTCTGT TTTCCACAAC TCGCTAAAGA TTTGTGAGAA CTATGGTCCA 
AACCTGGACA CCTGTCCAGA GGGGACAGTT TTGGGGCTGT TGGTGGATAG CAGTGGTTGT 
CTCCATTTGT TTGTGAATGG CATGGATCAG GGTGTAGCGG CACAGGACAT CCCGAGCCCC 
TGTTACGCCC TCATTGACCT TTACGGCCAG TGTGAACAGG TGACTATCGT GGCGAATCAT 
GTGCCGGTGG TTGGCGGTGA GCGAGGAGAC CTGCGCTGTC AAGGGGACAT GGAGAAGGCT 
GATATGGTGG ACGGTATAAA AGAGAGTGTC TGCTGGACTC CTCCTCCCGA GGTCAACCCT 
AATAAGACCT GCGAGTATCA GGCGCTGTGC TCCAGATTCA AAGACCTGCT CACACTGCCT 
GATGGTTACT TTAATGAGGA TGCCAAGTAT AACCTGTGTT ACTGTGAGTC CTGTCATAAA 
TTAAGGGGTG ATGAGGCCTA TTACAAGCGA GGAGAGCCCC CCAGAGACTA TGCTCTACCG 
TTCGGCTGGT GCCGCTTTGC ACTCCAGATC AAGACCCACT GTGAAGTCTC CAATGCCTTC 
AAGAAGTGGC ACATTGCGTA TCACGGCACA AGTGTCGGTT CTCTTAGACG CACCCTGGAC 
CACAACCAAC TGTTATCAGC AGAATCATCC TCTATTTTCT CGTCATCGTC GGTGAAGACG 
GAGGGTCATG GCAGCTACGG AGAGCCGGAG GAGAACAGCG CTCCAGAGAG AGACATTCCC 
AGAGTGCAGC TCTCACCAAC CATGCGATAT TCCGGCCTCG AGGTCTTCGC TCCGAAAGTG 
CAATTTCGAG ACCCGCGTTC GCTCCGCTGT CACCAGGCTC AGGTGGGCTT CCAGGTGTGT 
GTCCGGCCAG GCTCCTATAA AGTAGGTCCG TCGTCTTTAG GAATCAGCGA GCCGCTAGAC 
CCGCGATTCA GCAATGCAGA AATCGAATGG ATCACCAAGG AGAAAGGAGG CACGCTTCTA 
TATGGCCTGC TCATACGAGT TGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335985.1
CDS	317..5083
Translation	MAAELHPRSGKLIGLSNSNRTAQRNQPVQEFNHGLVLSREPLRDRDVFTVRIDKKVNSWSGSIEIGVTALDPAGLEFPSSATGLKGGSWIVSGCSVLKDGRSVLEEYGRDLDQLGEGDRVGIQRNGKGELHLWVNGQDCGAAASGLPSRLWAVVDLYGKCTQVTVVSCEPPSEMEGDEDEEVEDEIVSTQAVSIMADACRSHDDRAAVLTAAAATTERMNGSAEEVPEVSRTHSRPDKFPNNFEPDSVLTEHQLFDVFNNAIVSLYRSEDEGAEDLNVGGGGGGSDSSRGGTGSNGGGRGTGSDSGIGGGGGGGGSSSNSSPAGNAGGAAGLGVGTGGMTTNDALLFHEKCGTLIKLSNNNKTAERRRPLDEFNNGVVMTNRPLRHNEMFEIRIDKLVDKWSGSIEIGVTTHNPNNLDYPATMTNLRSGTIMMSGCGILTNGKGTRREYCEFSLDELQEGDHIGLMRKASGALHFYINGIDQGVAANQTPGVVYGVVDLYGMAVKVTIVHNHNHSDRLRRNNAIMRALSPDVGRPRPALSLTPEPDIPDRLLFHPNCGQKAAIISDGRTALRPHATDDFNHGVVLSNRPLHSNEVFQVRIDKMVDKWAGSIEIGVTTHNPAYLQLPSTMTNLRSGTWMMTGNGVMHNGTTILDEYGHNLDRLKAGDTVGVVRKEDGSLHFFVNGVPQGPAAWNVPPNVYAVVDLYGQAAQATIMDDMADLPPLPDDSSEGPTAMSPGSPCSISGATAANDLRFHQLHGTNAVITNGGRTALRQNCRSEFNDAIVISNRCLREGELFEIVIQKMVDRWSGSIEAGVTAIRPEELEFPNTMTDIDYDTWMLSGTAIMQDGNTMRNNYGCDLDSLTTASRIGMMRTATGDLHYFINGLDQGVACTGLPSEVYAVVDLYGQCVQVSITSSSGPLDNSLCTSNITEKSFPIHSPVAGVAHRLHSKHGKNVVLLGDGCQALRVGGYAHGIVFSAKELKTDEVFEVKINKVDERWSGSLHVGLTTLQPPDLPSCPLSGLSPSLTQLRTKVTWVLAGSEVRRNGVLQRQNYGCSLDRLTVGNRVGVKRCSDDTMHILIDGEDMGPAATAVAKNVYAVLDLYGKVTAVSIVSSTLVEDSESVKAPSLSSDSCSEGEEDCTPVRECENEPPLVPTVMTFLENHGKNIQLSNQNLTAARVSSYNQGLLVTAQALPRQQLFQFQIDRLNPSWTSSLSLGVIGHSPDRLNFPSTACCLKRSVWLLQRDSVFHNSLKICENYGPNLDTCPEGTVLGLLVDSSGCLHLFVNGMDQGVAAQDIPSPCYALIDLYGQCEQVTIVANHVPVVGGERGDLRCQGDMEKADMVDGIKESVCWTPPPEVNPNKTCEYQALCSRFKDLLTLPDGYFNEDAKYNLCYCESCHKLRGDEAYYKRGEPPRDYALPFGWCRFALQIKTHCEVSNAFKKWHIAYHGTSVGSLRRTLDHNQLLSAESSSIFSSSSVKTEGHGSYGEPEENSAPERDIPRVQLSPTMRYSGLEVFAPKVQFRDPRSLRCHQAQVGFQVCVRPGSYKVGPSSLGISEPLDPRFSNAEIEWITKEKGGTLLYGLLIRVE

Target ORF information:

RefSeq Version	XM_021480310.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neuralized E3 ubiquitin protein ligase 4 (neurl4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480310.1

ORF Insert Sequence:

ATGGCGGCTG AGCTGCATCC GCGCAGCGGA AAACTGATCG GTTTGTCGAA CTCCAACCGA 
ACCGCTCAAC GGAATCAGCC GGTACAGGAA TTCAATCACG GCCTGGTCCT CAGTCGGGAG 
CCGCTGAGGG ACCGCGATGT CTTCACTGTC CGCATCGACA AGAAGGTGAA CTCTTGGAGC 
GGCTCTATAG AAATCGGTGT GACGGCGCTG GACCCAGCCG GTCTGGAATT TCCCAGCAGC 
GCCACAGGGC TGAAAGGTGG CTCGTGGATT GTGTCTGGCT GCTCGGTGCT GAAGGACGGC 
CGTTCGGTCC TGGAGGAGTA CGGTCGGGAT CTTGACCAGC TTGGGGAAGG AGACCGTGTG 
GGCATCCAGC GGAATGGGAA AGGAGAGCTA CACCTGTGGG TGAATGGGCA GGACTGCGGC 
GCTGCTGCTA GTGGACTTCC ATCTCGACTG TGGGCTGTGG TGGACCTCTA TGGGAAGTGC 
ACACAAGTGA CTGTGGTGTC CTGTGAACCG CCATCTGAAA TGGAGGGTGA CGAGGATGAA 
GAGGTGGAGG ATGAGATTGT GTCGACGCAG GCAGTGTCCA TTATGGCAGA TGCCTGTCGT 
AGTCATGATG ATAGAGCTGC TGTACTGACT GCAGCTGCTG CTACTACAGA GCGGATGAAC 
GGATCAGCGG AGGAAGTGCC TGAAGTGTCA AGGACGCACA GTCGGCCGGA CAAATTTCCA 
AATAACTTTG AGCCTGATAG TGTTTTGACT GAGCATCAGT TGTTTGATGT GTTCAACAAT 
GCCATTGTGT CTCTGTATCG CTCGGAGGAT GAGGGGGCAG AGGATTTGAA TGTTGGGGGT 
GGAGGTGGCG GTTCTGACTC CTCAAGGGGC GGCACAGGAA GTAACGGTGG AGGCAGAGGA 
ACAGGAAGTG ACAGTGGCAT TGGCGGCGGA GGTGGTGGTG GAGGCAGTAG CAGCAATAGC 
AGCCCGGCGG GCAACGCTGG TGGGGCAGCA GGACTAGGGG TGGGCACTGG TGGAATGACG 
ACCAATGATG CACTGCTGTT TCATGAGAAA TGTGGGACGC TGATCAAACT GAGCAACAAT 
AATAAGACAG CAGAAAGAAG GAGACCGCTG GATGAGTTCA ATAATGGAGT CGTCATGACC 
AATCGACCAC TCAGGCACAA CGAAATGTTT GAGATTCGCA TTGACAAACT GGTGGATAAA 
TGGTCTGGTT CCATTGAGAT TGGAGTGACA ACCCACAACC CAAATAATCT TGATTACCCC 
GCAACAATGA CCAATTTGCG CTCAGGCACA ATAATGATGA GTGGCTGTGG GATTTTAACC 
AATGGGAAAG GGACTCGTCG GGAATACTGC GAATTCAGCC TGGATGAACT ACAGGAAGGA 
GATCACATTG GGCTGATGAG GAAAGCCAGT GGAGCACTGC ACTTCTACAT TAATGGCATT 
GATCAAGGGG TTGCAGCCAA CCAGACACCT GGCGTAGTGT ATGGAGTGGT GGATCTCTAT 
GGCATGGCGG TTAAAGTCAC CATTGTGCAC AACCATAACC ACAGTGATCG GTTGCGCCGC 
AATAACGCCA TCATGAGGGC ACTGTCACCT GACGTGGGTC GACCCCGGCC TGCACTCTCA 
TTAACCCCTG AGCCTGATAT CCCAGACCGC TTGCTCTTCC ATCCAAATTG TGGGCAGAAG 
GCTGCAATCA TCAGCGATGG ACGGACTGCT TTACGACCTC ATGCGACGGA TGACTTCAAC 
CATGGTGTTG TGCTTAGCAA TCGTCCATTG CACTCCAATG AGGTTTTCCA GGTGCGCATT 
GACAAAATGG TGGACAAATG GGCTGGCTCC ATAGAGATTG GTGTGACGAC TCATAACCCT 
GCCTATCTTC AGTTACCATC AACTATGACC AACCTGCGCT CGGGAACCTG GATGATGACT 
GGAAATGGGG TCATGCACAA TGGAACTACA ATTCTGGATG AATATGGGCA CAATTTGGAC 
CGTCTAAAAG CTGGAGATAC GGTGGGAGTT GTCCGAAAGG AAGATGGGAG TTTGCATTTC 
TTTGTGAATG GAGTGCCTCA AGGTCCTGCT GCCTGGAATG TTCCGCCCAA CGTGTATGCA 
GTAGTGGATC TGTATGGACA GGCTGCTCAG GCCACCATCA TGGATGATAT GGCTGATCTC 
CCTCCTTTGC CTGACGACAG CTCAGAGGGT CCCACTGCCA TGTCTCCTGG TAGCCCGTGT 
TCAATTTCAG GAGCCACAGC TGCAAATGAC CTGCGCTTTC ATCAGCTGCA TGGCACTAAC 
GCAGTCATCA CCAACGGTGG CCGAACAGCA CTTAGGCAGA ACTGCCGCAG CGAGTTTAAT 
GATGCCATCG TCATTTCAAA CAGGTGCCTT CGAGAAGGAG AGCTCTTTGA AATTGTGATT 
CAGAAAATGG TGGATCGCTG GTCGGGGTCT ATAGAAGCAG GAGTGACAGC GATCAGACCC 
GAAGAACTGG AGTTTCCAAA TACGATGACT GACATTGACT ATGACACATG GATGCTGAGC 
GGCACAGCAA TCATGCAGGA TGGCAATACC ATGAGAAATA ACTATGGCTG TGACTTAGAC 
TCTCTGACTA CTGCCTCCAG GATTGGGATG ATGCGAACAG CAACTGGAGA CCTGCATTAC 
TTCATCAACG GCCTCGACCA GGGTGTAGCT TGTACTGGGC TGCCATCAGA AGTGTATGCT 
GTGGTCGATC TTTATGGTCA GTGTGTGCAA GTGTCCATTA CAAGCTCATC CGGTCCACTG 
GACAACAGTC TGTGTACCAG TAACATCACA GAAAAGAGCT TCCCCATACA CTCCCCGGTG 
GCAGGAGTAG CTCATCGCCT GCACAGTAAA CATGGTAAAA ACGTGGTGTT ATTAGGAGAT 
GGATGCCAAG CGCTGAGGGT CGGAGGTTAT GCCCATGGAA TTGTCTTTAG TGCAAAAGAG 
CTCAAAACAG ATGAAGTGTT TGAGGTGAAA ATTAACAAGG TGGATGAGAG GTGGTCTGGT 
TCTCTTCATG TGGGGCTGAC CACCCTGCAG CCCCCTGACC TGCCATCATG CCCTCTCAGC 
GGCCTCTCGC CTTCACTCAC TCAGCTCAGG ACAAAGGTCA CCTGGGTGCT GGCCGGCTCT 
GAGGTCAGAC GCAACGGAGT ACTGCAGAGA CAGAACTATG GCTGCTCTCT GGATCGCCTC 
ACGGTTGGAA ATCGTGTCGG GGTGAAGAGA TGCAGTGACG ACACCATGCA CATTCTCATT 
GATGGAGAAG ATATGGGACC AGCAGCCACT GCAGTAGCAA AGAACGTGTA TGCTGTATTG 
GACCTGTATG GAAAGGTGAC GGCTGTGTCC ATCGTCAGTT CAACACTAGT TGAGGATTCT 
GAGAGTGTGA AAGCTCCCTC GCTCTCGTCC GACAGCTGCA GTGAAGGAGA AGAGGACTGC 
ACTCCTGTCA GAGAGTGTGA GAATGAGCCT CCATTGGTGC CCACAGTCAT GACGTTTTTG 
GAGAATCATG GGAAGAATAT CCAGCTGTCC AATCAAAATC TGACCGCCGC TCGGGTGTCC 
AGCTACAACC AGGGGCTTCT GGTCACAGCC CAAGCTCTGC CTCGCCAGCA GCTCTTCCAG 
TTTCAGATTG ACCGCTTGAA TCCCTCATGG ACGTCGTCTC TCTCCCTCGG TGTGATTGGT 
CATTCACCAG ACCGACTCAA CTTTCCTTCC ACAGCCTGCT GCCTTAAACG CTCAGTCTGG 
CTACTGCAAC GAGACTCTGT TTTCCACAAC TCGCTAAAGA TTTGTGAGAA CTATGGTCCA 
AACCTGGACA CCTGTCCAGA GGGGACAGTT TTGGGGCTGT TGGTGGATAG CAGTGGTTGT 
CTCCATTTGT TTGTGAATGG CATGGATCAG GGTGTAGCGG CACAGGACAT CCCGAGCCCC 
TGTTACGCCC TCATTGACCT TTACGGCCAG TGTGAACAGG TGACTATCGT GGCGAATCAT 
GTGCCGGTGG TTGGCGGTGA GCGAGGAGAC CTGCGCTGTC AAGGGGACAT GGAGAAGGCT 
GATATGGTGG ACGGTATAAA AGAGAGTGTC TGCTGGACTC CTCCTCCCGA GGTCAACCCT 
AATAAGACCT GCGAGTATCA GGCGCTGTGC TCCAGATTCA AAGACCTGCT CACACTGCCT 
GATGGTTACT TTAATGAGGA TGCCAAGTAT AACCTGTGTT ACTGTGAGTC CTGTCATAAA 
TTAAGGGGTG ATGAGGCCTA TTACAAGCGA GGAGAGCCCC CCAGAGACTA TGCTCTACCG 
TTCGGCTGGT GCCGCTTTGC ACTCCAGATC AAGACCCACT GTGAAGTCTC CAATGCCTTC 
AAGAAGTGGC ACATTGCGTA TCACGGCACA AGTGTCGGTT CTCTTAGACG CACCCTGGAC 
CACAACCAAC TGTTATCAGC AGAATCATCC TCTATTTTCT CGTCATCGTC GGTGAAGACG 
GAGGGTCATG GCAGCTACGG AGAGCCGGAG GAGAACAGCG CTCCAGAGAG AGACATTCCC 
AGAGTGCAGC TCTCACCAAC CATGCGATAT TCCGGCCTCG AGGTCTTCGC TCCGAAAGTG 
CAATTTCGAG ACCCGCGTTC GCTCCGCTGT CACCAGGCTC AGGTGGGCTT CCAGGTGTGT 
GTCCGGCCAG GCTCCTATAA AGTAGGTCCG TCGTCTTTAG GAATCAGCGA GCCGCTAGAC 
CCGCGATTCA GCAATGCAGA AATCGAATGG ATCACCAAGG AGAAAGGAGG CACGCTTCTA 
TATGGCCTGC TCATACGAGT TGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

neurl4 ( XM_005156224.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005156281.1 Danio rerio neuralized E3 ubiquitin protein ligase 4 (neurl4), transcript variant X2, mRNA.

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CloneID	ODa17891
Clone ID Related Accession (Same CDS sequence)	XM_005156224.4 , XM_005156224.3 , XM_021480311.1
Accession Version	XM_005156224.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neuralized-like protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394839.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005156224.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG AGCTGCATCC GCGCAGCGGA AAACTGATCG GTTTGTCGAA CTCCAACCGA 
ACCGCTCAAC GGAATCAGCC GGTACAGGAA TTCAATCACG GCCTGGTCCT CAGTCGGGAG 
CCGCTGAGGG ACCGCGATGT CTTCACTGTC CGCATCGACA AGAAGGTGAA CTCTTGGAGC 
GGCTCTATAG AAATCGGTGT GACGGCGCTG GACCCAGCCG GTCTGGAATT TCCCAGCAGC 
GCCACAGGGC TGAAAGGTGG CTCGTGGATT GTGTCTGGCT GCTCGGTGCT GAAGGACGGC 
CGTTCGGTCC TGGAGGAGTA CGGTCGGGAT CTTGACCAGC TTGGGGAAGG AGACCGTGTG 
GGCATCCAGC GGAATGGGAA AGGAGAGCTA CACCTGTGGG TGAATGGGCA GGACTGCGGC 
GCTGCTGCTA GTGGACTTCC ATCTCGACTG TGGGCTGTGG TGGACCTCTA TGGGAAGTGC 
ACACAAGTGA CTGTGGTGTC CTGTGAACCG CCATCTGAAA TGGAGGGTGA CGAGGATGAA 
GAGGTGGAGG ATGAGATTGT GTCGACGCAG GCAGTGTCCA TTATGGCAGA TGCCTGTCGT 
AGTCATGATG ATAGAGCTGC TGTACTGACT GCAGCTGCTG CTACTACAGA GCGGATGAAC 
GGATCAGCGG AGGAAGTGCC TGAAGTGTCA AGGACGCACA GTCGGCCGGA CAAATTTCCA 
AATAACTTTG AGCCTGATAG TGTTTTGACT GAGCATCAGT TGTTTGATGT GTTCAACAAT 
GCCATTGTGT CTCTGTATCG CTCGGAGGAT GAGGGGGCAG AGGATTTGAA TGTTGGGGGT 
GGAGGTGGCG GTTCTGACTC CTCAAGGGGC GGCACAGGAA GTAACGGTGG AGGCAGAGGA 
ACAGGAAGTG ACAGTGGCAT TGGCGGCGGA GGTGGTGGTG GAGGCAGTAG CAGCAATAGC 
AGCCCGGCGG GCAACGCTGG TGGGGCAGCA GGACTAGGGG TGGGCACTGG TGGAATGACG 
ACCAATGATG CACTGCTGTT TCATGAGAAA TGTGGGACGC TGATCAAACT GAGCAACAAT 
AATAAGACAG CAGAAAGAAG GAGACCGCTG GATGAGTTCA ATAATGGAGT CGTCATGACC 
AATCGACCAC TCAGGCACAA CGAAATGTTT GAGATTCGCA TTGACAAACT GGTGGATAAA 
TGGTCTGGTT CCATTGAGAT TGGAGTGACA ACCCACAACC CAAATAATCT TGATTACCCC 
GCAACAATGA CCAATTTGCG CTCAGGCACA ATAATGATGA GTGGCTGTGG GATTTTAACC 
AATGGGAAAG GGACTCGTCG GGAATACTGC GAATTCAGCC TGGATGAACT ACAGGAAGGA 
GATCACATTG GGCTGATGAG GAAAGCCAGT GGAGCACTGC ACTTCTACAT TAATGGCATT 
GATCAAGGGG TTGCAGCCAA CCAGACACCT GGCGTAGTGT ATGGAGTGGT GGATCTCTAT 
GGCATGGCGG TTAAAGTCAC CATTGTGCAC AACCATAACC ACAGTGATCG GTTGCGCCGC 
AATAACGCCA TCATGAGGGC ACTGTCACCT GACGTGGGTC GACCCCGGCC TGCACTCTCA 
TTAACCCCTG AGCCTGATAT CCCAGACCGC TTGCTCTTCC ATCCAAATTG TGGGCAGAAG 
GCTGCAATCA TCAGCGATGG ACGGACTGCT TTACGACCTC ATGCGACGGA TGACTTCAAC 
CATGGTGTTG TGCTTAGCAA TCGTCCATTG CACTCCAATG AGGTTTTCCA GGTGCGCATT 
GACAAAATGG TGGACAAATG GGCTGGCTCC ATAGAGATTG GTGTGACGAC TCATAACCCT 
GCCTATCTTC AGTTACCATC AACTATGACC AACCTGCGCT CGGGAACCTG GATGATGACT 
GGAAATGGGG TCATGCACAA TGGAACTACA ATTCTGGATG AATATGGGCA CAATTTGGAC 
CGTCTAAAAG CTGGAGATAC GGTGGGAGTT GTCCGAAAGG AAGATGGGAG TTTGCATTTC 
TTTGTGAATG GAGTGCCTCA AGGTCCTGCT GCCTGGAATG TTCCGCCCAA CGTGTATGCA 
GTAGTGGATC TGTATGGACA GGCTGCTCAG GCCACCATCA TGGATGATAT GGCTGATCTC 
CCTCCTTTGC CTGACGACAG CTCAGAGGGT CCCACTGCCA TGTCTCCTGG TAGCCCGTGT 
TCAATTTCAG GAGCCACAGC TGCAAATGAC CTGCGCTTTC ATCAGCTGCA TGGCACTAAC 
GCAGTCATCA CCAACGGTGG CCGAACAGCA CTTAGGCAGA ACTGCCGCAG CGAGTTTAAT 
GATGCCATCG TCATTTCAAA CAGGTGCCTT CGAGAAGGAG AGCTCTTTGA AATTGTGATT 
CAGAAAATGG TGGATCGCTG GTCGGGGTCT ATAGAAGCAG GAGTGACAGC GATCAGACCC 
GAAGAACTGG AGTTTCCAAA TACGATGACT GACATTGACT ATGACACATG GATGCTGAGC 
GGCACAGCAA TCATGCAGGA TGGCAATACC ATGAGAAATA ACTATGGCTG TGACTTAGAC 
TCTCTGACTA CTGCCTCCAG GATTGGGATG ATGCGAACAG CAACTGGAGA CCTGCATTAC 
TTCATCAACG GCCTCGACCA GGGTGTAGCT TGTACTGGGC TGCCATCAGA AGTGTATGCT 
GTGGTCGATC TTTATGGTCA GTGTGTGCAA GTGTCCATTA CAAGCTCATC CGGTCCACTG 
GACAACAGTC TGTGTACCAG TAACATCACA GAAAAGAGCT TCCCCATACA CTCCCCGGTG 
GCAGGAGTAG CTCATCGCCT GCACAGTAAA CATGGTAAAA ACGTGGTGTT ATTAGGAGAT 
GGATGCCAAG CGCTGAGGGT CGGAGGTTAT GCCCATGGAA TTGTCTTTAG TGCAAAAGAG 
CTCAAAACAG ATGAAGTGTT TGAGGTGAAA ATTAACAAGG TGGATGAGAG GTGGTCTGGT 
TCTCTTCATG TGGGGCTGAC CACCCTGCAG CCCCCTGACC TGCCATCATG CCCTCTCAGC 
GGCCTCTCGC CTTCACTCAC TCAGCTCAGG ACAAAGGTCA CCTGGGTGCT GGCCGGCTCT 
GAGGTCAGAC GCAACGGAGT ACTGCAGAGA CAGAACTATG GCTGCTCTCT GGATCGCCTC 
ACGGTTGGAA ATCGTGTCGG GGTGAAGAGA TGCAGTGACG ACACCATGCA CATTCTCATT 
GATGGAGAAG ATATGGGACC AGCAGCCACT GCAGTAGCAA AGAACGTGTA TGCTGTATTG 
GACCTGTATG GAAAGGTGAC GGCTGTGTCC ATCGTCAGTT CAACACTAGT TGAGGATTCT 
GAGAGTGTGA AAGCTCCCTC GCTCTCGTCC GACAGCTGCA GTGAAGGAGA AGAGGACTGC 
ACTCCTGTCA GAGAGTGTGA GAATGAGCCT CCATTGGTGC CCACAGTCAT GACGTTTTTG 
GAGAATCATG GGAAGAATAT CCAGCTGTCC AATCAAAATC TGACCGCCGC TCGGGTGTCC 
AGCTACAACC AGGGGCTTCT GGTCACAGCC CAAGCTCTGC CTCGCCAGCA GCTCTTCCAG 
TTTCAGATTG ACCGCTTGAA TCCCTCATGG ACGTCGTCTC TCTCCCTCGG TGTGATTGGT 
CATTCACCAG ACCGACTCAA CTTTCCTTCC ACAGCCTGCT GCCTTAAACG CTCAGTCTGG 
CTACTGCAAC GAGACTCTGT TTTCCACAAC TCGCTAAAGA TTTGTGAGAA CTATGGTCCA 
AACCTGGACA CCTGTCCAGA GGGGACAGTT TTGGGGCTGT TGGTGGATAG CAGTGGTTGT 
CTCCATTTGT TTGTGAATGG CATGGATCAG GGTGTAGCGG CACAGGACAT CCCGAGCCCC 
TGTTACGCCC TCATTGACCT TTACGGCCAG TGTGAACAGG TGACTATCGT GGCGAATCAT 
GTGCCGGTGG TTGGCGGTGA GCGAGGAGAC CTGCGCTGTC AAGGGGACAT GGAGAAGGCT 
GATATGGTGG ACGGTATAAA AGAGAGTGTC TGCTGGACTC CTCCTCCCGA GGTCAACCCT 
AATAAGACCT GCGAGTATCA GGCGCTGTGC TCCAGATTCA AAGACCTGCT CACACTGCCT 
GATGGTTACT TTAATGAGGA TGCCAAGTAT AACCTGTGTT ACTGTGAGTC CTGTCATAAA 
TTAAGGGGTG ATGAGGCCTA TTACAAGCGA GGAGAGCCCC CCAGAGACTA TGCTCTACCG 
TTCGGCTGGT GCCGCTTTGC ACTCCAGATC AAGACCCACT GTGAAGTCTC CAATGCCTTC 
AAGAAGTGGC ACATTGCGTA TCACGGCACA AGTGTCGGTT CTCTTAGACG CACCCTGGAC 
CACAACCAAC TGTTATCAGA ATCATCCTCT ATTTTCTCGT CATCGTCGGT GAAGACGGAG 
GGTCATGGCA GCTACGGAGA GCCGGAGGAG AACAGCGCTC CAGAGAGAGA CATTCCCAGA 
GTGCAGCTCT CACCAACCAT GCGATATTCC GGCCTCGAGG TCTTCGCTCC GAAAGTGCAA 
TTTCGAGACC CGCGTTCGCT CCGCTGTCAC CAGGCTCAGG TGGGCTTCCA GGTGTGTGTC 
CGGCCAGGCT CCTATAAAGT AGGTCCGTCG TCTTTAGGAA TCAGCGAGCC GCTAGACCCG 
CGATTCAGCA ATGCAGAAAT CGAATGGATC ACCAAGGAGA AAGGAGGCAC GCTTCTATAT 
GGCCTGCTCA TACGAGTTGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156281.1
CDS	317..5080
Translation	MAAELHPRSGKLIGLSNSNRTAQRNQPVQEFNHGLVLSREPLRDRDVFTVRIDKKVNSWSGSIEIGVTALDPAGLEFPSSATGLKGGSWIVSGCSVLKDGRSVLEEYGRDLDQLGEGDRVGIQRNGKGELHLWVNGQDCGAAASGLPSRLWAVVDLYGKCTQVTVVSCEPPSEMEGDEDEEVEDEIVSTQAVSIMADACRSHDDRAAVLTAAAATTERMNGSAEEVPEVSRTHSRPDKFPNNFEPDSVLTEHQLFDVFNNAIVSLYRSEDEGAEDLNVGGGGGGSDSSRGGTGSNGGGRGTGSDSGIGGGGGGGGSSSNSSPAGNAGGAAGLGVGTGGMTTNDALLFHEKCGTLIKLSNNNKTAERRRPLDEFNNGVVMTNRPLRHNEMFEIRIDKLVDKWSGSIEIGVTTHNPNNLDYPATMTNLRSGTIMMSGCGILTNGKGTRREYCEFSLDELQEGDHIGLMRKASGALHFYINGIDQGVAANQTPGVVYGVVDLYGMAVKVTIVHNHNHSDRLRRNNAIMRALSPDVGRPRPALSLTPEPDIPDRLLFHPNCGQKAAIISDGRTALRPHATDDFNHGVVLSNRPLHSNEVFQVRIDKMVDKWAGSIEIGVTTHNPAYLQLPSTMTNLRSGTWMMTGNGVMHNGTTILDEYGHNLDRLKAGDTVGVVRKEDGSLHFFVNGVPQGPAAWNVPPNVYAVVDLYGQAAQATIMDDMADLPPLPDDSSEGPTAMSPGSPCSISGATAANDLRFHQLHGTNAVITNGGRTALRQNCRSEFNDAIVISNRCLREGELFEIVIQKMVDRWSGSIEAGVTAIRPEELEFPNTMTDIDYDTWMLSGTAIMQDGNTMRNNYGCDLDSLTTASRIGMMRTATGDLHYFINGLDQGVACTGLPSEVYAVVDLYGQCVQVSITSSSGPLDNSLCTSNITEKSFPIHSPVAGVAHRLHSKHGKNVVLLGDGCQALRVGGYAHGIVFSAKELKTDEVFEVKINKVDERWSGSLHVGLTTLQPPDLPSCPLSGLSPSLTQLRTKVTWVLAGSEVRRNGVLQRQNYGCSLDRLTVGNRVGVKRCSDDTMHILIDGEDMGPAATAVAKNVYAVLDLYGKVTAVSIVSSTLVEDSESVKAPSLSSDSCSEGEEDCTPVRECENEPPLVPTVMTFLENHGKNIQLSNQNLTAARVSSYNQGLLVTAQALPRQQLFQFQIDRLNPSWTSSLSLGVIGHSPDRLNFPSTACCLKRSVWLLQRDSVFHNSLKICENYGPNLDTCPEGTVLGLLVDSSGCLHLFVNGMDQGVAAQDIPSPCYALIDLYGQCEQVTIVANHVPVVGGERGDLRCQGDMEKADMVDGIKESVCWTPPPEVNPNKTCEYQALCSRFKDLLTLPDGYFNEDAKYNLCYCESCHKLRGDEAYYKRGEPPRDYALPFGWCRFALQIKTHCEVSNAFKKWHIAYHGTSVGSLRRTLDHNQLLSESSSIFSSSSVKTEGHGSYGEPEENSAPERDIPRVQLSPTMRYSGLEVFAPKVQFRDPRSLRCHQAQVGFQVCVRPGSYKVGPSSLGISEPLDPRFSNAEIEWITKEKGGTLLYGLLIRVE

Target ORF information:

RefSeq Version	XM_005156224.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neuralized E3 ubiquitin protein ligase 4 (neurl4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156224.4

ORF Insert Sequence:

ATGGCGGCTG AGCTGCATCC GCGCAGCGGA AAACTGATCG GTTTGTCGAA CTCCAACCGA 
ACCGCTCAAC GGAATCAGCC GGTACAGGAA TTCAATCACG GCCTGGTCCT CAGTCGGGAG 
CCGCTGAGGG ACCGCGATGT CTTCACTGTC CGCATCGACA AGAAGGTGAA CTCTTGGAGC 
GGCTCTATAG AAATCGGTGT GACGGCGCTG GACCCAGCCG GTCTGGAATT TCCCAGCAGC 
GCCACAGGGC TGAAAGGTGG CTCGTGGATT GTGTCTGGCT GCTCGGTGCT GAAGGACGGC 
CGTTCGGTCC TGGAGGAGTA CGGTCGGGAT CTTGACCAGC TTGGGGAAGG AGACCGTGTG 
GGCATCCAGC GGAATGGGAA AGGAGAGCTA CACCTGTGGG TGAATGGGCA GGACTGCGGC 
GCTGCTGCTA GTGGACTTCC ATCTCGACTG TGGGCTGTGG TGGACCTCTA TGGGAAGTGC 
ACACAAGTGA CTGTGGTGTC CTGTGAACCG CCATCTGAAA TGGAGGGTGA CGAGGATGAA 
GAGGTGGAGG ATGAGATTGT GTCGACGCAG GCAGTGTCCA TTATGGCAGA TGCCTGTCGT 
AGTCATGATG ATAGAGCTGC TGTACTGACT GCAGCTGCTG CTACTACAGA GCGGATGAAC 
GGATCAGCGG AGGAAGTGCC TGAAGTGTCA AGGACGCACA GTCGGCCGGA CAAATTTCCA 
AATAACTTTG AGCCTGATAG TGTTTTGACT GAGCATCAGT TGTTTGATGT GTTCAACAAT 
GCCATTGTGT CTCTGTATCG CTCGGAGGAT GAGGGGGCAG AGGATTTGAA TGTTGGGGGT 
GGAGGTGGCG GTTCTGACTC CTCAAGGGGC GGCACAGGAA GTAACGGTGG AGGCAGAGGA 
ACAGGAAGTG ACAGTGGCAT TGGCGGCGGA GGTGGTGGTG GAGGCAGTAG CAGCAATAGC 
AGCCCGGCGG GCAACGCTGG TGGGGCAGCA GGACTAGGGG TGGGCACTGG TGGAATGACG 
ACCAATGATG CACTGCTGTT TCATGAGAAA TGTGGGACGC TGATCAAACT GAGCAACAAT 
AATAAGACAG CAGAAAGAAG GAGACCGCTG GATGAGTTCA ATAATGGAGT CGTCATGACC 
AATCGACCAC TCAGGCACAA CGAAATGTTT GAGATTCGCA TTGACAAACT GGTGGATAAA 
TGGTCTGGTT CCATTGAGAT TGGAGTGACA ACCCACAACC CAAATAATCT TGATTACCCC 
GCAACAATGA CCAATTTGCG CTCAGGCACA ATAATGATGA GTGGCTGTGG GATTTTAACC 
AATGGGAAAG GGACTCGTCG GGAATACTGC GAATTCAGCC TGGATGAACT ACAGGAAGGA 
GATCACATTG GGCTGATGAG GAAAGCCAGT GGAGCACTGC ACTTCTACAT TAATGGCATT 
GATCAAGGGG TTGCAGCCAA CCAGACACCT GGCGTAGTGT ATGGAGTGGT GGATCTCTAT 
GGCATGGCGG TTAAAGTCAC CATTGTGCAC AACCATAACC ACAGTGATCG GTTGCGCCGC 
AATAACGCCA TCATGAGGGC ACTGTCACCT GACGTGGGTC GACCCCGGCC TGCACTCTCA 
TTAACCCCTG AGCCTGATAT CCCAGACCGC TTGCTCTTCC ATCCAAATTG TGGGCAGAAG 
GCTGCAATCA TCAGCGATGG ACGGACTGCT TTACGACCTC ATGCGACGGA TGACTTCAAC 
CATGGTGTTG TGCTTAGCAA TCGTCCATTG CACTCCAATG AGGTTTTCCA GGTGCGCATT 
GACAAAATGG TGGACAAATG GGCTGGCTCC ATAGAGATTG GTGTGACGAC TCATAACCCT 
GCCTATCTTC AGTTACCATC AACTATGACC AACCTGCGCT CGGGAACCTG GATGATGACT 
GGAAATGGGG TCATGCACAA TGGAACTACA ATTCTGGATG AATATGGGCA CAATTTGGAC 
CGTCTAAAAG CTGGAGATAC GGTGGGAGTT GTCCGAAAGG AAGATGGGAG TTTGCATTTC 
TTTGTGAATG GAGTGCCTCA AGGTCCTGCT GCCTGGAATG TTCCGCCCAA CGTGTATGCA 
GTAGTGGATC TGTATGGACA GGCTGCTCAG GCCACCATCA TGGATGATAT GGCTGATCTC 
CCTCCTTTGC CTGACGACAG CTCAGAGGGT CCCACTGCCA TGTCTCCTGG TAGCCCGTGT 
TCAATTTCAG GAGCCACAGC TGCAAATGAC CTGCGCTTTC ATCAGCTGCA TGGCACTAAC 
GCAGTCATCA CCAACGGTGG CCGAACAGCA CTTAGGCAGA ACTGCCGCAG CGAGTTTAAT 
GATGCCATCG TCATTTCAAA CAGGTGCCTT CGAGAAGGAG AGCTCTTTGA AATTGTGATT 
CAGAAAATGG TGGATCGCTG GTCGGGGTCT ATAGAAGCAG GAGTGACAGC GATCAGACCC 
GAAGAACTGG AGTTTCCAAA TACGATGACT GACATTGACT ATGACACATG GATGCTGAGC 
GGCACAGCAA TCATGCAGGA TGGCAATACC ATGAGAAATA ACTATGGCTG TGACTTAGAC 
TCTCTGACTA CTGCCTCCAG GATTGGGATG ATGCGAACAG CAACTGGAGA CCTGCATTAC 
TTCATCAACG GCCTCGACCA GGGTGTAGCT TGTACTGGGC TGCCATCAGA AGTGTATGCT 
GTGGTCGATC TTTATGGTCA GTGTGTGCAA GTGTCCATTA CAAGCTCATC CGGTCCACTG 
GACAACAGTC TGTGTACCAG TAACATCACA GAAAAGAGCT TCCCCATACA CTCCCCGGTG 
GCAGGAGTAG CTCATCGCCT GCACAGTAAA CATGGTAAAA ACGTGGTGTT ATTAGGAGAT 
GGATGCCAAG CGCTGAGGGT CGGAGGTTAT GCCCATGGAA TTGTCTTTAG TGCAAAAGAG 
CTCAAAACAG ATGAAGTGTT TGAGGTGAAA ATTAACAAGG TGGATGAGAG GTGGTCTGGT 
TCTCTTCATG TGGGGCTGAC CACCCTGCAG CCCCCTGACC TGCCATCATG CCCTCTCAGC 
GGCCTCTCGC CTTCACTCAC TCAGCTCAGG ACAAAGGTCA CCTGGGTGCT GGCCGGCTCT 
GAGGTCAGAC GCAACGGAGT ACTGCAGAGA CAGAACTATG GCTGCTCTCT GGATCGCCTC 
ACGGTTGGAA ATCGTGTCGG GGTGAAGAGA TGCAGTGACG ACACCATGCA CATTCTCATT 
GATGGAGAAG ATATGGGACC AGCAGCCACT GCAGTAGCAA AGAACGTGTA TGCTGTATTG 
GACCTGTATG GAAAGGTGAC GGCTGTGTCC ATCGTCAGTT CAACACTAGT TGAGGATTCT 
GAGAGTGTGA AAGCTCCCTC GCTCTCGTCC GACAGCTGCA GTGAAGGAGA AGAGGACTGC 
ACTCCTGTCA GAGAGTGTGA GAATGAGCCT CCATTGGTGC CCACAGTCAT GACGTTTTTG 
GAGAATCATG GGAAGAATAT CCAGCTGTCC AATCAAAATC TGACCGCCGC TCGGGTGTCC 
AGCTACAACC AGGGGCTTCT GGTCACAGCC CAAGCTCTGC CTCGCCAGCA GCTCTTCCAG 
TTTCAGATTG ACCGCTTGAA TCCCTCATGG ACGTCGTCTC TCTCCCTCGG TGTGATTGGT 
CATTCACCAG ACCGACTCAA CTTTCCTTCC ACAGCCTGCT GCCTTAAACG CTCAGTCTGG 
CTACTGCAAC GAGACTCTGT TTTCCACAAC TCGCTAAAGA TTTGTGAGAA CTATGGTCCA 
AACCTGGACA CCTGTCCAGA GGGGACAGTT TTGGGGCTGT TGGTGGATAG CAGTGGTTGT 
CTCCATTTGT TTGTGAATGG CATGGATCAG GGTGTAGCGG CACAGGACAT CCCGAGCCCC 
TGTTACGCCC TCATTGACCT TTACGGCCAG TGTGAACAGG TGACTATCGT GGCGAATCAT 
GTGCCGGTGG TTGGCGGTGA GCGAGGAGAC CTGCGCTGTC AAGGGGACAT GGAGAAGGCT 
GATATGGTGG ACGGTATAAA AGAGAGTGTC TGCTGGACTC CTCCTCCCGA GGTCAACCCT 
AATAAGACCT GCGAGTATCA GGCGCTGTGC TCCAGATTCA AAGACCTGCT CACACTGCCT 
GATGGTTACT TTAATGAGGA TGCCAAGTAT AACCTGTGTT ACTGTGAGTC CTGTCATAAA 
TTAAGGGGTG ATGAGGCCTA TTACAAGCGA GGAGAGCCCC CCAGAGACTA TGCTCTACCG 
TTCGGCTGGT GCCGCTTTGC ACTCCAGATC AAGACCCACT GTGAAGTCTC CAATGCCTTC 
AAGAAGTGGC ACATTGCGTA TCACGGCACA AGTGTCGGTT CTCTTAGACG CACCCTGGAC 
CACAACCAAC TGTTATCAGA ATCATCCTCT ATTTTCTCGT CATCGTCGGT GAAGACGGAG 
GGTCATGGCA GCTACGGAGA GCCGGAGGAG AACAGCGCTC CAGAGAGAGA CATTCCCAGA 
GTGCAGCTCT CACCAACCAT GCGATATTCC GGCCTCGAGG TCTTCGCTCC GAAAGTGCAA 
TTTCGAGACC CGCGTTCGCT CCGCTGTCAC CAGGCTCAGG TGGGCTTCCA GGTGTGTGTC 
CGGCCAGGCT CCTATAAAGT AGGTCCGTCG TCTTTAGGAA TCAGCGAGCC GCTAGACCCG 
CGATTCAGCA ATGCAGAAAT CGAATGGATC ACCAAGGAGA AAGGAGGCAC GCTTCTATAT 
GGCCTGCTCA TACGAGTTGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

neurl4 ( XM_005156224.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005156281.1 Danio rerio neuralized E3 ubiquitin protein ligase 4 (neurl4), transcript variant X2, mRNA.

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CloneID	ODa17891
Clone ID Related Accession (Same CDS sequence)	XM_005156224.4 , XM_005156224.3 , XM_021480311.1
Accession Version	XM_005156224.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	neuralized-like protein 4 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005156224.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG AGCTGCATCC GCGCAGCGGA AAACTGATCG GTTTGTCGAA CTCCAACCGA 
ACCGCTCAAC GGAATCAGCC GGTACAGGAA TTCAATCACG GCCTGGTCCT CAGTCGGGAG 
CCGCTGAGGG ACCGCGATGT CTTCACTGTC CGCATCGACA AGAAGGTGAA CTCTTGGAGC 
GGCTCTATAG AAATCGGTGT GACGGCGCTG GACCCAGCCG GTCTGGAATT TCCCAGCAGC 
GCCACAGGGC TGAAAGGTGG CTCGTGGATT GTGTCTGGCT GCTCGGTGCT GAAGGACGGC 
CGTTCGGTCC TGGAGGAGTA CGGTCGGGAT CTTGACCAGC TTGGGGAAGG AGACCGTGTG 
GGCATCCAGC GGAATGGGAA AGGAGAGCTA CACCTGTGGG TGAATGGGCA GGACTGCGGC 
GCTGCTGCTA GTGGACTTCC ATCTCGACTG TGGGCTGTGG TGGACCTCTA TGGGAAGTGC 
ACACAAGTGA CTGTGGTGTC CTGTGAACCG CCATCTGAAA TGGAGGGTGA CGAGGATGAA 
GAGGTGGAGG ATGAGATTGT GTCGACGCAG GCAGTGTCCA TTATGGCAGA TGCCTGTCGT 
AGTCATGATG ATAGAGCTGC TGTACTGACT GCAGCTGCTG CTACTACAGA GCGGATGAAC 
GGATCAGCGG AGGAAGTGCC TGAAGTGTCA AGGACGCACA GTCGGCCGGA CAAATTTCCA 
AATAACTTTG AGCCTGATAG TGTTTTGACT GAGCATCAGT TGTTTGATGT GTTCAACAAT 
GCCATTGTGT CTCTGTATCG CTCGGAGGAT GAGGGGGCAG AGGATTTGAA TGTTGGGGGT 
GGAGGTGGCG GTTCTGACTC CTCAAGGGGC GGCACAGGAA GTAACGGTGG AGGCAGAGGA 
ACAGGAAGTG ACAGTGGCAT TGGCGGCGGA GGTGGTGGTG GAGGCAGTAG CAGCAATAGC 
AGCCCGGCGG GCAACGCTGG TGGGGCAGCA GGACTAGGGG TGGGCACTGG TGGAATGACG 
ACCAATGATG CACTGCTGTT TCATGAGAAA TGTGGGACGC TGATCAAACT GAGCAACAAT 
AATAAGACAG CAGAAAGAAG GAGACCGCTG GATGAGTTCA ATAATGGAGT CGTCATGACC 
AATCGACCAC TCAGGCACAA CGAAATGTTT GAGATTCGCA TTGACAAACT GGTGGATAAA 
TGGTCTGGTT CCATTGAGAT TGGAGTGACA ACCCACAACC CAAATAATCT TGATTACCCC 
GCAACAATGA CCAATTTGCG CTCAGGCACA ATAATGATGA GTGGCTGTGG GATTTTAACC 
AATGGGAAAG GGACTCGTCG GGAATACTGC GAATTCAGCC TGGATGAACT ACAGGAAGGA 
GATCACATTG GGCTGATGAG GAAAGCCAGT GGAGCACTGC ACTTCTACAT TAATGGCATT 
GATCAAGGGG TTGCAGCCAA CCAGACACCT GGCGTAGTGT ATGGAGTGGT GGATCTCTAT 
GGCATGGCGG TTAAAGTCAC CATTGTGCAC AACCATAACC ACAGTGATCG GTTGCGCCGC 
AATAACGCCA TCATGAGGGC ACTGTCACCT GACGTGGGTC GACCCCGGCC TGCACTCTCA 
TTAACCCCTG AGCCTGATAT CCCAGACCGC TTGCTCTTCC ATCCAAATTG TGGGCAGAAG 
GCTGCAATCA TCAGCGATGG ACGGACTGCT TTACGACCTC ATGCGACGGA TGACTTCAAC 
CATGGTGTTG TGCTTAGCAA TCGTCCATTG CACTCCAATG AGGTTTTCCA GGTGCGCATT 
GACAAAATGG TGGACAAATG GGCTGGCTCC ATAGAGATTG GTGTGACGAC TCATAACCCT 
GCCTATCTTC AGTTACCATC AACTATGACC AACCTGCGCT CGGGAACCTG GATGATGACT 
GGAAATGGGG TCATGCACAA TGGAACTACA ATTCTGGATG AATATGGGCA CAATTTGGAC 
CGTCTAAAAG CTGGAGATAC GGTGGGAGTT GTCCGAAAGG AAGATGGGAG TTTGCATTTC 
TTTGTGAATG GAGTGCCTCA AGGTCCTGCT GCCTGGAATG TTCCGCCCAA CGTGTATGCA 
GTAGTGGATC TGTATGGACA GGCTGCTCAG GCCACCATCA TGGATGATAT GGCTGATCTC 
CCTCCTTTGC CTGACGACAG CTCAGAGGGT CCCACTGCCA TGTCTCCTGG TAGCCCGTGT 
TCAATTTCAG GAGCCACAGC TGCAAATGAC CTGCGCTTTC ATCAGCTGCA TGGCACTAAC 
GCAGTCATCA CCAACGGTGG CCGAACAGCA CTTAGGCAGA ACTGCCGCAG CGAGTTTAAT 
GATGCCATCG TCATTTCAAA CAGGTGCCTT CGAGAAGGAG AGCTCTTTGA AATTGTGATT 
CAGAAAATGG TGGATCGCTG GTCGGGGTCT ATAGAAGCAG GAGTGACAGC GATCAGACCC 
GAAGAACTGG AGTTTCCAAA TACGATGACT GACATTGACT ATGACACATG GATGCTGAGC 
GGCACAGCAA TCATGCAGGA TGGCAATACC ATGAGAAATA ACTATGGCTG TGACTTAGAC 
TCTCTGACTA CTGCCTCCAG GATTGGGATG ATGCGAACAG CAACTGGAGA CCTGCATTAC 
TTCATCAACG GCCTCGACCA GGGTGTAGCT TGTACTGGGC TGCCATCAGA AGTGTATGCT 
GTGGTCGATC TTTATGGTCA GTGTGTGCAA GTGTCCATTA CAAGCTCATC CGGTCCACTG 
GACAACAGTC TGTGTACCAG TAACATCACA GAAAAGAGCT TCCCCATACA CTCCCCGGTG 
GCAGGAGTAG CTCATCGCCT GCACAGTAAA CATGGTAAAA ACGTGGTGTT ATTAGGAGAT 
GGATGCCAAG CGCTGAGGGT CGGAGGTTAT GCCCATGGAA TTGTCTTTAG TGCAAAAGAG 
CTCAAAACAG ATGAAGTGTT TGAGGTGAAA ATTAACAAGG TGGATGAGAG GTGGTCTGGT 
TCTCTTCATG TGGGGCTGAC CACCCTGCAG CCCCCTGACC TGCCATCATG CCCTCTCAGC 
GGCCTCTCGC CTTCACTCAC TCAGCTCAGG ACAAAGGTCA CCTGGGTGCT GGCCGGCTCT 
GAGGTCAGAC GCAACGGAGT ACTGCAGAGA CAGAACTATG GCTGCTCTCT GGATCGCCTC 
ACGGTTGGAA ATCGTGTCGG GGTGAAGAGA TGCAGTGACG ACACCATGCA CATTCTCATT 
GATGGAGAAG ATATGGGACC AGCAGCCACT GCAGTAGCAA AGAACGTGTA TGCTGTATTG 
GACCTGTATG GAAAGGTGAC GGCTGTGTCC ATCGTCAGTT CAACACTAGT TGAGGATTCT 
GAGAGTGTGA AAGCTCCCTC GCTCTCGTCC GACAGCTGCA GTGAAGGAGA AGAGGACTGC 
ACTCCTGTCA GAGAGTGTGA GAATGAGCCT CCATTGGTGC CCACAGTCAT GACGTTTTTG 
GAGAATCATG GGAAGAATAT CCAGCTGTCC AATCAAAATC TGACCGCCGC TCGGGTGTCC 
AGCTACAACC AGGGGCTTCT GGTCACAGCC CAAGCTCTGC CTCGCCAGCA GCTCTTCCAG 
TTTCAGATTG ACCGCTTGAA TCCCTCATGG ACGTCGTCTC TCTCCCTCGG TGTGATTGGT 
CATTCACCAG ACCGACTCAA CTTTCCTTCC ACAGCCTGCT GCCTTAAACG CTCAGTCTGG 
CTACTGCAAC GAGACTCTGT TTTCCACAAC TCGCTAAAGA TTTGTGAGAA CTATGGTCCA 
AACCTGGACA CCTGTCCAGA GGGGACAGTT TTGGGGCTGT TGGTGGATAG CAGTGGTTGT 
CTCCATTTGT TTGTGAATGG CATGGATCAG GGTGTAGCGG CACAGGACAT CCCGAGCCCC 
TGTTACGCCC TCATTGACCT TTACGGCCAG TGTGAACAGG TGACTATCGT GGCGAATCAT 
GTGCCGGTGG TTGGCGGTGA GCGAGGAGAC CTGCGCTGTC AAGGGGACAT GGAGAAGGCT 
GATATGGTGG ACGGTATAAA AGAGAGTGTC TGCTGGACTC CTCCTCCCGA GGTCAACCCT 
AATAAGACCT GCGAGTATCA GGCGCTGTGC TCCAGATTCA AAGACCTGCT CACACTGCCT 
GATGGTTACT TTAATGAGGA TGCCAAGTAT AACCTGTGTT ACTGTGAGTC CTGTCATAAA 
TTAAGGGGTG ATGAGGCCTA TTACAAGCGA GGAGAGCCCC CCAGAGACTA TGCTCTACCG 
TTCGGCTGGT GCCGCTTTGC ACTCCAGATC AAGACCCACT GTGAAGTCTC CAATGCCTTC 
AAGAAGTGGC ACATTGCGTA TCACGGCACA AGTGTCGGTT CTCTTAGACG CACCCTGGAC 
CACAACCAAC TGTTATCAGA ATCATCCTCT ATTTTCTCGT CATCGTCGGT GAAGACGGAG 
GGTCATGGCA GCTACGGAGA GCCGGAGGAG AACAGCGCTC CAGAGAGAGA CATTCCCAGA 
GTGCAGCTCT CACCAACCAT GCGATATTCC GGCCTCGAGG TCTTCGCTCC GAAAGTGCAA 
TTTCGAGACC CGCGTTCGCT CCGCTGTCAC CAGGCTCAGG TGGGCTTCCA GGTGTGTGTC 
CGGCCAGGCT CCTATAAAGT AGGTCCGTCG TCTTTAGGAA TCAGCGAGCC GCTAGACCCG 
CGATTCAGCA ATGCAGAAAT CGAATGGATC ACCAAGGAGA AAGGAGGCAC GCTTCTATAT 
GGCCTGCTCA TACGAGTTGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156281.1
CDS	74..4837
Translation	MAAELHPRSGKLIGLSNSNRTAQRNQPVQEFNHGLVLSREPLRDRDVFTVRIDKKVNSWSGSIEIGVTALDPAGLEFPSSATGLKGGSWIVSGCSVLKDGRSVLEEYGRDLDQLGEGDRVGIQRNGKGELHLWVNGQDCGAAASGLPSRLWAVVDLYGKCTQVTVVSCEPPSEMEGDEDEEVEDEIVSTQAVSIMADACRSHDDRAAVLTAAAATTERMNGSAEEVPEVSRTHSRPDKFPNNFEPDSVLTEHQLFDVFNNAIVSLYRSEDEGAEDLNVGGGGGGSDSSRGGTGSNGGGRGTGSDSGIGGGGGGGGSSSNSSPAGNAGGAAGLGVGTGGMTTNDALLFHEKCGTLIKLSNNNKTAERRRPLDEFNNGVVMTNRPLRHNEMFEIRIDKLVDKWSGSIEIGVTTHNPNNLDYPATMTNLRSGTIMMSGCGILTNGKGTRREYCEFSLDELQEGDHIGLMRKASGALHFYINGIDQGVAANQTPGVVYGVVDLYGMAVKVTIVHNHNHSDRLRRNNAIMRALSPDVGRPRPALSLTPEPDIPDRLLFHPNCGQKAAIISDGRTALRPHATDDFNHGVVLSNRPLHSNEVFQVRIDKMVDKWAGSIEIGVTTHNPAYLQLPSTMTNLRSGTWMMTGNGVMHNGTTILDEYGHNLDRLKAGDTVGVVRKEDGSLHFFVNGVPQGPAAWNVPPNVYAVVDLYGQAAQATIMDDMADLPPLPDDSSEGPTAMSPGSPCSISGATAANDLRFHQLHGTNAVITNGGRTALRQNCRSEFNDAIVISNRCLREGELFEIVIQKMVDRWSGSIEAGVTAIRPEELEFPNTMTDIDYDTWMLSGTAIMQDGNTMRNNYGCDLDSLTTASRIGMMRTATGDLHYFINGLDQGVACTGLPSEVYAVVDLYGQCVQVSITSSSGPLDNSLCTSNITEKSFPIHSPVAGVAHRLHSKHGKNVVLLGDGCQALRVGGYAHGIVFSAKELKTDEVFEVKINKVDERWSGSLHVGLTTLQPPDLPSCPLSGLSPSLTQLRTKVTWVLAGSEVRRNGVLQRQNYGCSLDRLTVGNRVGVKRCSDDTMHILIDGEDMGPAATAVAKNVYAVLDLYGKVTAVSIVSSTLVEDSESVKAPSLSSDSCSEGEEDCTPVRECENEPPLVPTVMTFLENHGKNIQLSNQNLTAARVSSYNQGLLVTAQALPRQQLFQFQIDRLNPSWTSSLSLGVIGHSPDRLNFPSTACCLKRSVWLLQRDSVFHNSLKICENYGPNLDTCPEGTVLGLLVDSSGCLHLFVNGMDQGVAAQDIPSPCYALIDLYGQCEQVTIVANHVPVVGGERGDLRCQGDMEKADMVDGIKESVCWTPPPEVNPNKTCEYQALCSRFKDLLTLPDGYFNEDAKYNLCYCESCHKLRGDEAYYKRGEPPRDYALPFGWCRFALQIKTHCEVSNAFKKWHIAYHGTSVGSLRRTLDHNQLLSESSSIFSSSSVKTEGHGSYGEPEENSAPERDIPRVQLSPTMRYSGLEVFAPKVQFRDPRSLRCHQAQVGFQVCVRPGSYKVGPSSLGISEPLDPRFSNAEIEWITKEKGGTLLYGLLIRVE

Target ORF information:

RefSeq Version	XM_005156224.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neuralized E3 ubiquitin protein ligase 4 (neurl4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156224.3

ORF Insert Sequence:

ATGGCGGCTG AGCTGCATCC GCGCAGCGGA AAACTGATCG GTTTGTCGAA CTCCAACCGA 
ACCGCTCAAC GGAATCAGCC GGTACAGGAA TTCAATCACG GCCTGGTCCT CAGTCGGGAG 
CCGCTGAGGG ACCGCGATGT CTTCACTGTC CGCATCGACA AGAAGGTGAA CTCTTGGAGC 
GGCTCTATAG AAATCGGTGT GACGGCGCTG GACCCAGCCG GTCTGGAATT TCCCAGCAGC 
GCCACAGGGC TGAAAGGTGG CTCGTGGATT GTGTCTGGCT GCTCGGTGCT GAAGGACGGC 
CGTTCGGTCC TGGAGGAGTA CGGTCGGGAT CTTGACCAGC TTGGGGAAGG AGACCGTGTG 
GGCATCCAGC GGAATGGGAA AGGAGAGCTA CACCTGTGGG TGAATGGGCA GGACTGCGGC 
GCTGCTGCTA GTGGACTTCC ATCTCGACTG TGGGCTGTGG TGGACCTCTA TGGGAAGTGC 
ACACAAGTGA CTGTGGTGTC CTGTGAACCG CCATCTGAAA TGGAGGGTGA CGAGGATGAA 
GAGGTGGAGG ATGAGATTGT GTCGACGCAG GCAGTGTCCA TTATGGCAGA TGCCTGTCGT 
AGTCATGATG ATAGAGCTGC TGTACTGACT GCAGCTGCTG CTACTACAGA GCGGATGAAC 
GGATCAGCGG AGGAAGTGCC TGAAGTGTCA AGGACGCACA GTCGGCCGGA CAAATTTCCA 
AATAACTTTG AGCCTGATAG TGTTTTGACT GAGCATCAGT TGTTTGATGT GTTCAACAAT 
GCCATTGTGT CTCTGTATCG CTCGGAGGAT GAGGGGGCAG AGGATTTGAA TGTTGGGGGT 
GGAGGTGGCG GTTCTGACTC CTCAAGGGGC GGCACAGGAA GTAACGGTGG AGGCAGAGGA 
ACAGGAAGTG ACAGTGGCAT TGGCGGCGGA GGTGGTGGTG GAGGCAGTAG CAGCAATAGC 
AGCCCGGCGG GCAACGCTGG TGGGGCAGCA GGACTAGGGG TGGGCACTGG TGGAATGACG 
ACCAATGATG CACTGCTGTT TCATGAGAAA TGTGGGACGC TGATCAAACT GAGCAACAAT 
AATAAGACAG CAGAAAGAAG GAGACCGCTG GATGAGTTCA ATAATGGAGT CGTCATGACC 
AATCGACCAC TCAGGCACAA CGAAATGTTT GAGATTCGCA TTGACAAACT GGTGGATAAA 
TGGTCTGGTT CCATTGAGAT TGGAGTGACA ACCCACAACC CAAATAATCT TGATTACCCC 
GCAACAATGA CCAATTTGCG CTCAGGCACA ATAATGATGA GTGGCTGTGG GATTTTAACC 
AATGGGAAAG GGACTCGTCG GGAATACTGC GAATTCAGCC TGGATGAACT ACAGGAAGGA 
GATCACATTG GGCTGATGAG GAAAGCCAGT GGAGCACTGC ACTTCTACAT TAATGGCATT 
GATCAAGGGG TTGCAGCCAA CCAGACACCT GGCGTAGTGT ATGGAGTGGT GGATCTCTAT 
GGCATGGCGG TTAAAGTCAC CATTGTGCAC AACCATAACC ACAGTGATCG GTTGCGCCGC 
AATAACGCCA TCATGAGGGC ACTGTCACCT GACGTGGGTC GACCCCGGCC TGCACTCTCA 
TTAACCCCTG AGCCTGATAT CCCAGACCGC TTGCTCTTCC ATCCAAATTG TGGGCAGAAG 
GCTGCAATCA TCAGCGATGG ACGGACTGCT TTACGACCTC ATGCGACGGA TGACTTCAAC 
CATGGTGTTG TGCTTAGCAA TCGTCCATTG CACTCCAATG AGGTTTTCCA GGTGCGCATT 
GACAAAATGG TGGACAAATG GGCTGGCTCC ATAGAGATTG GTGTGACGAC TCATAACCCT 
GCCTATCTTC AGTTACCATC AACTATGACC AACCTGCGCT CGGGAACCTG GATGATGACT 
GGAAATGGGG TCATGCACAA TGGAACTACA ATTCTGGATG AATATGGGCA CAATTTGGAC 
CGTCTAAAAG CTGGAGATAC GGTGGGAGTT GTCCGAAAGG AAGATGGGAG TTTGCATTTC 
TTTGTGAATG GAGTGCCTCA AGGTCCTGCT GCCTGGAATG TTCCGCCCAA CGTGTATGCA 
GTAGTGGATC TGTATGGACA GGCTGCTCAG GCCACCATCA TGGATGATAT GGCTGATCTC 
CCTCCTTTGC CTGACGACAG CTCAGAGGGT CCCACTGCCA TGTCTCCTGG TAGCCCGTGT 
TCAATTTCAG GAGCCACAGC TGCAAATGAC CTGCGCTTTC ATCAGCTGCA TGGCACTAAC 
GCAGTCATCA CCAACGGTGG CCGAACAGCA CTTAGGCAGA ACTGCCGCAG CGAGTTTAAT 
GATGCCATCG TCATTTCAAA CAGGTGCCTT CGAGAAGGAG AGCTCTTTGA AATTGTGATT 
CAGAAAATGG TGGATCGCTG GTCGGGGTCT ATAGAAGCAG GAGTGACAGC GATCAGACCC 
GAAGAACTGG AGTTTCCAAA TACGATGACT GACATTGACT ATGACACATG GATGCTGAGC 
GGCACAGCAA TCATGCAGGA TGGCAATACC ATGAGAAATA ACTATGGCTG TGACTTAGAC 
TCTCTGACTA CTGCCTCCAG GATTGGGATG ATGCGAACAG CAACTGGAGA CCTGCATTAC 
TTCATCAACG GCCTCGACCA GGGTGTAGCT TGTACTGGGC TGCCATCAGA AGTGTATGCT 
GTGGTCGATC TTTATGGTCA GTGTGTGCAA GTGTCCATTA CAAGCTCATC CGGTCCACTG 
GACAACAGTC TGTGTACCAG TAACATCACA GAAAAGAGCT TCCCCATACA CTCCCCGGTG 
GCAGGAGTAG CTCATCGCCT GCACAGTAAA CATGGTAAAA ACGTGGTGTT ATTAGGAGAT 
GGATGCCAAG CGCTGAGGGT CGGAGGTTAT GCCCATGGAA TTGTCTTTAG TGCAAAAGAG 
CTCAAAACAG ATGAAGTGTT TGAGGTGAAA ATTAACAAGG TGGATGAGAG GTGGTCTGGT 
TCTCTTCATG TGGGGCTGAC CACCCTGCAG CCCCCTGACC TGCCATCATG CCCTCTCAGC 
GGCCTCTCGC CTTCACTCAC TCAGCTCAGG ACAAAGGTCA CCTGGGTGCT GGCCGGCTCT 
GAGGTCAGAC GCAACGGAGT ACTGCAGAGA CAGAACTATG GCTGCTCTCT GGATCGCCTC 
ACGGTTGGAA ATCGTGTCGG GGTGAAGAGA TGCAGTGACG ACACCATGCA CATTCTCATT 
GATGGAGAAG ATATGGGACC AGCAGCCACT GCAGTAGCAA AGAACGTGTA TGCTGTATTG 
GACCTGTATG GAAAGGTGAC GGCTGTGTCC ATCGTCAGTT CAACACTAGT TGAGGATTCT 
GAGAGTGTGA AAGCTCCCTC GCTCTCGTCC GACAGCTGCA GTGAAGGAGA AGAGGACTGC 
ACTCCTGTCA GAGAGTGTGA GAATGAGCCT CCATTGGTGC CCACAGTCAT GACGTTTTTG 
GAGAATCATG GGAAGAATAT CCAGCTGTCC AATCAAAATC TGACCGCCGC TCGGGTGTCC 
AGCTACAACC AGGGGCTTCT GGTCACAGCC CAAGCTCTGC CTCGCCAGCA GCTCTTCCAG 
TTTCAGATTG ACCGCTTGAA TCCCTCATGG ACGTCGTCTC TCTCCCTCGG TGTGATTGGT 
CATTCACCAG ACCGACTCAA CTTTCCTTCC ACAGCCTGCT GCCTTAAACG CTCAGTCTGG 
CTACTGCAAC GAGACTCTGT TTTCCACAAC TCGCTAAAGA TTTGTGAGAA CTATGGTCCA 
AACCTGGACA CCTGTCCAGA GGGGACAGTT TTGGGGCTGT TGGTGGATAG CAGTGGTTGT 
CTCCATTTGT TTGTGAATGG CATGGATCAG GGTGTAGCGG CACAGGACAT CCCGAGCCCC 
TGTTACGCCC TCATTGACCT TTACGGCCAG TGTGAACAGG TGACTATCGT GGCGAATCAT 
GTGCCGGTGG TTGGCGGTGA GCGAGGAGAC CTGCGCTGTC AAGGGGACAT GGAGAAGGCT 
GATATGGTGG ACGGTATAAA AGAGAGTGTC TGCTGGACTC CTCCTCCCGA GGTCAACCCT 
AATAAGACCT GCGAGTATCA GGCGCTGTGC TCCAGATTCA AAGACCTGCT CACACTGCCT 
GATGGTTACT TTAATGAGGA TGCCAAGTAT AACCTGTGTT ACTGTGAGTC CTGTCATAAA 
TTAAGGGGTG ATGAGGCCTA TTACAAGCGA GGAGAGCCCC CCAGAGACTA TGCTCTACCG 
TTCGGCTGGT GCCGCTTTGC ACTCCAGATC AAGACCCACT GTGAAGTCTC CAATGCCTTC 
AAGAAGTGGC ACATTGCGTA TCACGGCACA AGTGTCGGTT CTCTTAGACG CACCCTGGAC 
CACAACCAAC TGTTATCAGA ATCATCCTCT ATTTTCTCGT CATCGTCGGT GAAGACGGAG 
GGTCATGGCA GCTACGGAGA GCCGGAGGAG AACAGCGCTC CAGAGAGAGA CATTCCCAGA 
GTGCAGCTCT CACCAACCAT GCGATATTCC GGCCTCGAGG TCTTCGCTCC GAAAGTGCAA 
TTTCGAGACC CGCGTTCGCT CCGCTGTCAC CAGGCTCAGG TGGGCTTCCA GGTGTGTGTC 
CGGCCAGGCT CCTATAAAGT AGGTCCGTCG TCTTTAGGAA TCAGCGAGCC GCTAGACCCG 
CGATTCAGCA ATGCAGAAAT CGAATGGATC ACCAAGGAGA AAGGAGGCAC GCTTCTATAT 
GGCCTGCTCA TACGAGTTGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

neurl4 ( XM_021480311.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335986.1 Danio rerio neuralized E3 ubiquitin protein ligase 4 (neurl4), transcript variant X2, mRNA.

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CloneID	ODa17891
Clone ID Related Accession (Same CDS sequence)	XM_005156224.4 , XM_005156224.3 , XM_021480311.1
Accession Version	XM_021480311.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neuralized-like protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG AGCTGCATCC GCGCAGCGGA AAACTGATCG GTTTGTCGAA CTCCAACCGA 
ACCGCTCAAC GGAATCAGCC GGTACAGGAA TTCAATCACG GCCTGGTCCT CAGTCGGGAG 
CCGCTGAGGG ACCGCGATGT CTTCACTGTC CGCATCGACA AGAAGGTGAA CTCTTGGAGC 
GGCTCTATAG AAATCGGTGT GACGGCGCTG GACCCAGCCG GTCTGGAATT TCCCAGCAGC 
GCCACAGGGC TGAAAGGTGG CTCGTGGATT GTGTCTGGCT GCTCGGTGCT GAAGGACGGC 
CGTTCGGTCC TGGAGGAGTA CGGTCGGGAT CTTGACCAGC TTGGGGAAGG AGACCGTGTG 
GGCATCCAGC GGAATGGGAA AGGAGAGCTA CACCTGTGGG TGAATGGGCA GGACTGCGGC 
GCTGCTGCTA GTGGACTTCC ATCTCGACTG TGGGCTGTGG TGGACCTCTA TGGGAAGTGC 
ACACAAGTGA CTGTGGTGTC CTGTGAACCG CCATCTGAAA TGGAGGGTGA CGAGGATGAA 
GAGGTGGAGG ATGAGATTGT GTCGACGCAG GCAGTGTCCA TTATGGCAGA TGCCTGTCGT 
AGTCATGATG ATAGAGCTGC TGTACTGACT GCAGCTGCTG CTACTACAGA GCGGATGAAC 
GGATCAGCGG AGGAAGTGCC TGAAGTGTCA AGGACGCACA GTCGGCCGGA CAAATTTCCA 
AATAACTTTG AGCCTGATAG TGTTTTGACT GAGCATCAGT TGTTTGATGT GTTCAACAAT 
GCCATTGTGT CTCTGTATCG CTCGGAGGAT GAGGGGGCAG AGGATTTGAA TGTTGGGGGT 
GGAGGTGGCG GTTCTGACTC CTCAAGGGGC GGCACAGGAA GTAACGGTGG AGGCAGAGGA 
ACAGGAAGTG ACAGTGGCAT TGGCGGCGGA GGTGGTGGTG GAGGCAGTAG CAGCAATAGC 
AGCCCGGCGG GCAACGCTGG TGGGGCAGCA GGACTAGGGG TGGGCACTGG TGGAATGACG 
ACCAATGATG CACTGCTGTT TCATGAGAAA TGTGGGACGC TGATCAAACT GAGCAACAAT 
AATAAGACAG CAGAAAGAAG GAGACCGCTG GATGAGTTCA ATAATGGAGT CGTCATGACC 
AATCGACCAC TCAGGCACAA CGAAATGTTT GAGATTCGCA TTGACAAACT GGTGGATAAA 
TGGTCTGGTT CCATTGAGAT TGGAGTGACA ACCCACAACC CAAATAATCT TGATTACCCC 
GCAACAATGA CCAATTTGCG CTCAGGCACA ATAATGATGA GTGGCTGTGG GATTTTAACC 
AATGGGAAAG GGACTCGTCG GGAATACTGC GAATTCAGCC TGGATGAACT ACAGGAAGGA 
GATCACATTG GGCTGATGAG GAAAGCCAGT GGAGCACTGC ACTTCTACAT TAATGGCATT 
GATCAAGGGG TTGCAGCCAA CCAGACACCT GGCGTAGTGT ATGGAGTGGT GGATCTCTAT 
GGCATGGCGG TTAAAGTCAC CATTGTGCAC AACCATAACC ACAGTGATCG GTTGCGCCGC 
AATAACGCCA TCATGAGGGC ACTGTCACCT GACGTGGGTC GACCCCGGCC TGCACTCTCA 
TTAACCCCTG AGCCTGATAT CCCAGACCGC TTGCTCTTCC ATCCAAATTG TGGGCAGAAG 
GCTGCAATCA TCAGCGATGG ACGGACTGCT TTACGACCTC ATGCGACGGA TGACTTCAAC 
CATGGTGTTG TGCTTAGCAA TCGTCCATTG CACTCCAATG AGGTTTTCCA GGTGCGCATT 
GACAAAATGG TGGACAAATG GGCTGGCTCC ATAGAGATTG GTGTGACGAC TCATAACCCT 
GCCTATCTTC AGTTACCATC AACTATGACC AACCTGCGCT CGGGAACCTG GATGATGACT 
GGAAATGGGG TCATGCACAA TGGAACTACA ATTCTGGATG AATATGGGCA CAATTTGGAC 
CGTCTAAAAG CTGGAGATAC GGTGGGAGTT GTCCGAAAGG AAGATGGGAG TTTGCATTTC 
TTTGTGAATG GAGTGCCTCA AGGTCCTGCT GCCTGGAATG TTCCGCCCAA CGTGTATGCA 
GTAGTGGATC TGTATGGACA GGCTGCTCAG GCCACCATCA TGGATGATAT GGCTGATCTC 
CCTCCTTTGC CTGACGACAG CTCAGAGGGT CCCACTGCCA TGTCTCCTGG TAGCCCGTGT 
TCAATTTCAG GAGCCACAGC TGCAAATGAC CTGCGCTTTC ATCAGCTGCA TGGCACTAAC 
GCAGTCATCA CCAACGGTGG CCGAACAGCA CTTAGGCAGA ACTGCCGCAG CGAGTTTAAT 
GATGCCATCG TCATTTCAAA CAGGTGCCTT CGAGAAGGAG AGCTCTTTGA AATTGTGATT 
CAGAAAATGG TGGATCGCTG GTCGGGGTCT ATAGAAGCAG GAGTGACAGC GATCAGACCC 
GAAGAACTGG AGTTTCCAAA TACGATGACT GACATTGACT ATGACACATG GATGCTGAGC 
GGCACAGCAA TCATGCAGGA TGGCAATACC ATGAGAAATA ACTATGGCTG TGACTTAGAC 
TCTCTGACTA CTGCCTCCAG GATTGGGATG ATGCGAACAG CAACTGGAGA CCTGCATTAC 
TTCATCAACG GCCTCGACCA GGGTGTAGCT TGTACTGGGC TGCCATCAGA AGTGTATGCT 
GTGGTCGATC TTTATGGTCA GTGTGTGCAA GTGTCCATTA CAAGCTCATC CGGTCCACTG 
GACAACAGTC TGTGTACCAG TAACATCACA GAAAAGAGCT TCCCCATACA CTCCCCGGTG 
GCAGGAGTAG CTCATCGCCT GCACAGTAAA CATGGTAAAA ACGTGGTGTT ATTAGGAGAT 
GGATGCCAAG CGCTGAGGGT CGGAGGTTAT GCCCATGGAA TTGTCTTTAG TGCAAAAGAG 
CTCAAAACAG ATGAAGTGTT TGAGGTGAAA ATTAACAAGG TGGATGAGAG GTGGTCTGGT 
TCTCTTCATG TGGGGCTGAC CACCCTGCAG CCCCCTGACC TGCCATCATG CCCTCTCAGC 
GGCCTCTCGC CTTCACTCAC TCAGCTCAGG ACAAAGGTCA CCTGGGTGCT GGCCGGCTCT 
GAGGTCAGAC GCAACGGAGT ACTGCAGAGA CAGAACTATG GCTGCTCTCT GGATCGCCTC 
ACGGTTGGAA ATCGTGTCGG GGTGAAGAGA TGCAGTGACG ACACCATGCA CATTCTCATT 
GATGGAGAAG ATATGGGACC AGCAGCCACT GCAGTAGCAA AGAACGTGTA TGCTGTATTG 
GACCTGTATG GAAAGGTGAC GGCTGTGTCC ATCGTCAGTT CAACACTAGT TGAGGATTCT 
GAGAGTGTGA AAGCTCCCTC GCTCTCGTCC GACAGCTGCA GTGAAGGAGA AGAGGACTGC 
ACTCCTGTCA GAGAGTGTGA GAATGAGCCT CCATTGGTGC CCACAGTCAT GACGTTTTTG 
GAGAATCATG GGAAGAATAT CCAGCTGTCC AATCAAAATC TGACCGCCGC TCGGGTGTCC 
AGCTACAACC AGGGGCTTCT GGTCACAGCC CAAGCTCTGC CTCGCCAGCA GCTCTTCCAG 
TTTCAGATTG ACCGCTTGAA TCCCTCATGG ACGTCGTCTC TCTCCCTCGG TGTGATTGGT 
CATTCACCAG ACCGACTCAA CTTTCCTTCC ACAGCCTGCT GCCTTAAACG CTCAGTCTGG 
CTACTGCAAC GAGACTCTGT TTTCCACAAC TCGCTAAAGA TTTGTGAGAA CTATGGTCCA 
AACCTGGACA CCTGTCCAGA GGGGACAGTT TTGGGGCTGT TGGTGGATAG CAGTGGTTGT 
CTCCATTTGT TTGTGAATGG CATGGATCAG GGTGTAGCGG CACAGGACAT CCCGAGCCCC 
TGTTACGCCC TCATTGACCT TTACGGCCAG TGTGAACAGG TGACTATCGT GGCGAATCAT 
GTGCCGGTGG TTGGCGGTGA GCGAGGAGAC CTGCGCTGTC AAGGGGACAT GGAGAAGGCT 
GATATGGTGG ACGGTATAAA AGAGAGTGTC TGCTGGACTC CTCCTCCCGA GGTCAACCCT 
AATAAGACCT GCGAGTATCA GGCGCTGTGC TCCAGATTCA AAGACCTGCT CACACTGCCT 
GATGGTTACT TTAATGAGGA TGCCAAGTAT AACCTGTGTT ACTGTGAGTC CTGTCATAAA 
TTAAGGGGTG ATGAGGCCTA TTACAAGCGA GGAGAGCCCC CCAGAGACTA TGCTCTACCG 
TTCGGCTGGT GCCGCTTTGC ACTCCAGATC AAGACCCACT GTGAAGTCTC CAATGCCTTC 
AAGAAGTGGC ACATTGCGTA TCACGGCACA AGTGTCGGTT CTCTTAGACG CACCCTGGAC 
CACAACCAAC TGTTATCAGA ATCATCCTCT ATTTTCTCGT CATCGTCGGT GAAGACGGAG 
GGTCATGGCA GCTACGGAGA GCCGGAGGAG AACAGCGCTC CAGAGAGAGA CATTCCCAGA 
GTGCAGCTCT CACCAACCAT GCGATATTCC GGCCTCGAGG TCTTCGCTCC GAAAGTGCAA 
TTTCGAGACC CGCGTTCGCT CCGCTGTCAC CAGGCTCAGG TGGGCTTCCA GGTGTGTGTC 
CGGCCAGGCT CCTATAAAGT AGGTCCGTCG TCTTTAGGAA TCAGCGAGCC GCTAGACCCG 
CGATTCAGCA ATGCAGAAAT CGAATGGATC ACCAAGGAGA AAGGAGGCAC GCTTCTATAT 
GGCCTGCTCA TACGAGTTGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335986.1
CDS	317..5080
Translation	MAAELHPRSGKLIGLSNSNRTAQRNQPVQEFNHGLVLSREPLRDRDVFTVRIDKKVNSWSGSIEIGVTALDPAGLEFPSSATGLKGGSWIVSGCSVLKDGRSVLEEYGRDLDQLGEGDRVGIQRNGKGELHLWVNGQDCGAAASGLPSRLWAVVDLYGKCTQVTVVSCEPPSEMEGDEDEEVEDEIVSTQAVSIMADACRSHDDRAAVLTAAAATTERMNGSAEEVPEVSRTHSRPDKFPNNFEPDSVLTEHQLFDVFNNAIVSLYRSEDEGAEDLNVGGGGGGSDSSRGGTGSNGGGRGTGSDSGIGGGGGGGGSSSNSSPAGNAGGAAGLGVGTGGMTTNDALLFHEKCGTLIKLSNNNKTAERRRPLDEFNNGVVMTNRPLRHNEMFEIRIDKLVDKWSGSIEIGVTTHNPNNLDYPATMTNLRSGTIMMSGCGILTNGKGTRREYCEFSLDELQEGDHIGLMRKASGALHFYINGIDQGVAANQTPGVVYGVVDLYGMAVKVTIVHNHNHSDRLRRNNAIMRALSPDVGRPRPALSLTPEPDIPDRLLFHPNCGQKAAIISDGRTALRPHATDDFNHGVVLSNRPLHSNEVFQVRIDKMVDKWAGSIEIGVTTHNPAYLQLPSTMTNLRSGTWMMTGNGVMHNGTTILDEYGHNLDRLKAGDTVGVVRKEDGSLHFFVNGVPQGPAAWNVPPNVYAVVDLYGQAAQATIMDDMADLPPLPDDSSEGPTAMSPGSPCSISGATAANDLRFHQLHGTNAVITNGGRTALRQNCRSEFNDAIVISNRCLREGELFEIVIQKMVDRWSGSIEAGVTAIRPEELEFPNTMTDIDYDTWMLSGTAIMQDGNTMRNNYGCDLDSLTTASRIGMMRTATGDLHYFINGLDQGVACTGLPSEVYAVVDLYGQCVQVSITSSSGPLDNSLCTSNITEKSFPIHSPVAGVAHRLHSKHGKNVVLLGDGCQALRVGGYAHGIVFSAKELKTDEVFEVKINKVDERWSGSLHVGLTTLQPPDLPSCPLSGLSPSLTQLRTKVTWVLAGSEVRRNGVLQRQNYGCSLDRLTVGNRVGVKRCSDDTMHILIDGEDMGPAATAVAKNVYAVLDLYGKVTAVSIVSSTLVEDSESVKAPSLSSDSCSEGEEDCTPVRECENEPPLVPTVMTFLENHGKNIQLSNQNLTAARVSSYNQGLLVTAQALPRQQLFQFQIDRLNPSWTSSLSLGVIGHSPDRLNFPSTACCLKRSVWLLQRDSVFHNSLKICENYGPNLDTCPEGTVLGLLVDSSGCLHLFVNGMDQGVAAQDIPSPCYALIDLYGQCEQVTIVANHVPVVGGERGDLRCQGDMEKADMVDGIKESVCWTPPPEVNPNKTCEYQALCSRFKDLLTLPDGYFNEDAKYNLCYCESCHKLRGDEAYYKRGEPPRDYALPFGWCRFALQIKTHCEVSNAFKKWHIAYHGTSVGSLRRTLDHNQLLSESSSIFSSSSVKTEGHGSYGEPEENSAPERDIPRVQLSPTMRYSGLEVFAPKVQFRDPRSLRCHQAQVGFQVCVRPGSYKVGPSSLGISEPLDPRFSNAEIEWITKEKGGTLLYGLLIRVE

Target ORF information:

RefSeq Version	XM_021480311.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neuralized E3 ubiquitin protein ligase 4 (neurl4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480311.1

ORF Insert Sequence:

ATGGCGGCTG AGCTGCATCC GCGCAGCGGA AAACTGATCG GTTTGTCGAA CTCCAACCGA 
ACCGCTCAAC GGAATCAGCC GGTACAGGAA TTCAATCACG GCCTGGTCCT CAGTCGGGAG 
CCGCTGAGGG ACCGCGATGT CTTCACTGTC CGCATCGACA AGAAGGTGAA CTCTTGGAGC 
GGCTCTATAG AAATCGGTGT GACGGCGCTG GACCCAGCCG GTCTGGAATT TCCCAGCAGC 
GCCACAGGGC TGAAAGGTGG CTCGTGGATT GTGTCTGGCT GCTCGGTGCT GAAGGACGGC 
CGTTCGGTCC TGGAGGAGTA CGGTCGGGAT CTTGACCAGC TTGGGGAAGG AGACCGTGTG 
GGCATCCAGC GGAATGGGAA AGGAGAGCTA CACCTGTGGG TGAATGGGCA GGACTGCGGC 
GCTGCTGCTA GTGGACTTCC ATCTCGACTG TGGGCTGTGG TGGACCTCTA TGGGAAGTGC 
ACACAAGTGA CTGTGGTGTC CTGTGAACCG CCATCTGAAA TGGAGGGTGA CGAGGATGAA 
GAGGTGGAGG ATGAGATTGT GTCGACGCAG GCAGTGTCCA TTATGGCAGA TGCCTGTCGT 
AGTCATGATG ATAGAGCTGC TGTACTGACT GCAGCTGCTG CTACTACAGA GCGGATGAAC 
GGATCAGCGG AGGAAGTGCC TGAAGTGTCA AGGACGCACA GTCGGCCGGA CAAATTTCCA 
AATAACTTTG AGCCTGATAG TGTTTTGACT GAGCATCAGT TGTTTGATGT GTTCAACAAT 
GCCATTGTGT CTCTGTATCG CTCGGAGGAT GAGGGGGCAG AGGATTTGAA TGTTGGGGGT 
GGAGGTGGCG GTTCTGACTC CTCAAGGGGC GGCACAGGAA GTAACGGTGG AGGCAGAGGA 
ACAGGAAGTG ACAGTGGCAT TGGCGGCGGA GGTGGTGGTG GAGGCAGTAG CAGCAATAGC 
AGCCCGGCGG GCAACGCTGG TGGGGCAGCA GGACTAGGGG TGGGCACTGG TGGAATGACG 
ACCAATGATG CACTGCTGTT TCATGAGAAA TGTGGGACGC TGATCAAACT GAGCAACAAT 
AATAAGACAG CAGAAAGAAG GAGACCGCTG GATGAGTTCA ATAATGGAGT CGTCATGACC 
AATCGACCAC TCAGGCACAA CGAAATGTTT GAGATTCGCA TTGACAAACT GGTGGATAAA 
TGGTCTGGTT CCATTGAGAT TGGAGTGACA ACCCACAACC CAAATAATCT TGATTACCCC 
GCAACAATGA CCAATTTGCG CTCAGGCACA ATAATGATGA GTGGCTGTGG GATTTTAACC 
AATGGGAAAG GGACTCGTCG GGAATACTGC GAATTCAGCC TGGATGAACT ACAGGAAGGA 
GATCACATTG GGCTGATGAG GAAAGCCAGT GGAGCACTGC ACTTCTACAT TAATGGCATT 
GATCAAGGGG TTGCAGCCAA CCAGACACCT GGCGTAGTGT ATGGAGTGGT GGATCTCTAT 
GGCATGGCGG TTAAAGTCAC CATTGTGCAC AACCATAACC ACAGTGATCG GTTGCGCCGC 
AATAACGCCA TCATGAGGGC ACTGTCACCT GACGTGGGTC GACCCCGGCC TGCACTCTCA 
TTAACCCCTG AGCCTGATAT CCCAGACCGC TTGCTCTTCC ATCCAAATTG TGGGCAGAAG 
GCTGCAATCA TCAGCGATGG ACGGACTGCT TTACGACCTC ATGCGACGGA TGACTTCAAC 
CATGGTGTTG TGCTTAGCAA TCGTCCATTG CACTCCAATG AGGTTTTCCA GGTGCGCATT 
GACAAAATGG TGGACAAATG GGCTGGCTCC ATAGAGATTG GTGTGACGAC TCATAACCCT 
GCCTATCTTC AGTTACCATC AACTATGACC AACCTGCGCT CGGGAACCTG GATGATGACT 
GGAAATGGGG TCATGCACAA TGGAACTACA ATTCTGGATG AATATGGGCA CAATTTGGAC 
CGTCTAAAAG CTGGAGATAC GGTGGGAGTT GTCCGAAAGG AAGATGGGAG TTTGCATTTC 
TTTGTGAATG GAGTGCCTCA AGGTCCTGCT GCCTGGAATG TTCCGCCCAA CGTGTATGCA 
GTAGTGGATC TGTATGGACA GGCTGCTCAG GCCACCATCA TGGATGATAT GGCTGATCTC 
CCTCCTTTGC CTGACGACAG CTCAGAGGGT CCCACTGCCA TGTCTCCTGG TAGCCCGTGT 
TCAATTTCAG GAGCCACAGC TGCAAATGAC CTGCGCTTTC ATCAGCTGCA TGGCACTAAC 
GCAGTCATCA CCAACGGTGG CCGAACAGCA CTTAGGCAGA ACTGCCGCAG CGAGTTTAAT 
GATGCCATCG TCATTTCAAA CAGGTGCCTT CGAGAAGGAG AGCTCTTTGA AATTGTGATT 
CAGAAAATGG TGGATCGCTG GTCGGGGTCT ATAGAAGCAG GAGTGACAGC GATCAGACCC 
GAAGAACTGG AGTTTCCAAA TACGATGACT GACATTGACT ATGACACATG GATGCTGAGC 
GGCACAGCAA TCATGCAGGA TGGCAATACC ATGAGAAATA ACTATGGCTG TGACTTAGAC 
TCTCTGACTA CTGCCTCCAG GATTGGGATG ATGCGAACAG CAACTGGAGA CCTGCATTAC 
TTCATCAACG GCCTCGACCA GGGTGTAGCT TGTACTGGGC TGCCATCAGA AGTGTATGCT 
GTGGTCGATC TTTATGGTCA GTGTGTGCAA GTGTCCATTA CAAGCTCATC CGGTCCACTG 
GACAACAGTC TGTGTACCAG TAACATCACA GAAAAGAGCT TCCCCATACA CTCCCCGGTG 
GCAGGAGTAG CTCATCGCCT GCACAGTAAA CATGGTAAAA ACGTGGTGTT ATTAGGAGAT 
GGATGCCAAG CGCTGAGGGT CGGAGGTTAT GCCCATGGAA TTGTCTTTAG TGCAAAAGAG 
CTCAAAACAG ATGAAGTGTT TGAGGTGAAA ATTAACAAGG TGGATGAGAG GTGGTCTGGT 
TCTCTTCATG TGGGGCTGAC CACCCTGCAG CCCCCTGACC TGCCATCATG CCCTCTCAGC 
GGCCTCTCGC CTTCACTCAC TCAGCTCAGG ACAAAGGTCA CCTGGGTGCT GGCCGGCTCT 
GAGGTCAGAC GCAACGGAGT ACTGCAGAGA CAGAACTATG GCTGCTCTCT GGATCGCCTC 
ACGGTTGGAA ATCGTGTCGG GGTGAAGAGA TGCAGTGACG ACACCATGCA CATTCTCATT 
GATGGAGAAG ATATGGGACC AGCAGCCACT GCAGTAGCAA AGAACGTGTA TGCTGTATTG 
GACCTGTATG GAAAGGTGAC GGCTGTGTCC ATCGTCAGTT CAACACTAGT TGAGGATTCT 
GAGAGTGTGA AAGCTCCCTC GCTCTCGTCC GACAGCTGCA GTGAAGGAGA AGAGGACTGC 
ACTCCTGTCA GAGAGTGTGA GAATGAGCCT CCATTGGTGC CCACAGTCAT GACGTTTTTG 
GAGAATCATG GGAAGAATAT CCAGCTGTCC AATCAAAATC TGACCGCCGC TCGGGTGTCC 
AGCTACAACC AGGGGCTTCT GGTCACAGCC CAAGCTCTGC CTCGCCAGCA GCTCTTCCAG 
TTTCAGATTG ACCGCTTGAA TCCCTCATGG ACGTCGTCTC TCTCCCTCGG TGTGATTGGT 
CATTCACCAG ACCGACTCAA CTTTCCTTCC ACAGCCTGCT GCCTTAAACG CTCAGTCTGG 
CTACTGCAAC GAGACTCTGT TTTCCACAAC TCGCTAAAGA TTTGTGAGAA CTATGGTCCA 
AACCTGGACA CCTGTCCAGA GGGGACAGTT TTGGGGCTGT TGGTGGATAG CAGTGGTTGT 
CTCCATTTGT TTGTGAATGG CATGGATCAG GGTGTAGCGG CACAGGACAT CCCGAGCCCC 
TGTTACGCCC TCATTGACCT TTACGGCCAG TGTGAACAGG TGACTATCGT GGCGAATCAT 
GTGCCGGTGG TTGGCGGTGA GCGAGGAGAC CTGCGCTGTC AAGGGGACAT GGAGAAGGCT 
GATATGGTGG ACGGTATAAA AGAGAGTGTC TGCTGGACTC CTCCTCCCGA GGTCAACCCT 
AATAAGACCT GCGAGTATCA GGCGCTGTGC TCCAGATTCA AAGACCTGCT CACACTGCCT 
GATGGTTACT TTAATGAGGA TGCCAAGTAT AACCTGTGTT ACTGTGAGTC CTGTCATAAA 
TTAAGGGGTG ATGAGGCCTA TTACAAGCGA GGAGAGCCCC CCAGAGACTA TGCTCTACCG 
TTCGGCTGGT GCCGCTTTGC ACTCCAGATC AAGACCCACT GTGAAGTCTC CAATGCCTTC 
AAGAAGTGGC ACATTGCGTA TCACGGCACA AGTGTCGGTT CTCTTAGACG CACCCTGGAC 
CACAACCAAC TGTTATCAGA ATCATCCTCT ATTTTCTCGT CATCGTCGGT GAAGACGGAG 
GGTCATGGCA GCTACGGAGA GCCGGAGGAG AACAGCGCTC CAGAGAGAGA CATTCCCAGA 
GTGCAGCTCT CACCAACCAT GCGATATTCC GGCCTCGAGG TCTTCGCTCC GAAAGTGCAA 
TTTCGAGACC CGCGTTCGCT CCGCTGTCAC CAGGCTCAGG TGGGCTTCCA GGTGTGTGTC 
CGGCCAGGCT CCTATAAAGT AGGTCCGTCG TCTTTAGGAA TCAGCGAGCC GCTAGACCCG 
CGATTCAGCA ATGCAGAAAT CGAATGGATC ACCAAGGAGA AAGGAGGCAC GCTTCTATAT 
GGCCTGCTCA TACGAGTTGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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neurl4 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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