AZIN1A cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	AZIN1A
Entrez Gene ID	550230
Full Name	antizyme inhibitor 1a
Synonyms	wu:fc93f10,zgc:110131
General protein information	Preferred Names antizyme inhibitor 1a Names antizyme inhibitor 1 ornithine decarboxylase antizyme inhibitor
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)
Summary	The protein encoded by this gene belongs to the antizyme inhibitor family, which plays a role in cell growth and proliferation by maintaining polyamine homeostasis within the cell. Antizyme inhibitors are homologs of ornithine decarboxylase (ODC, the key enzyme in polyamine biosynthesis) that have lost the ability to decarboxylase ornithine; however, retain the ability to bind to antizymes. Antizymes negatively regulate intracellular polyamine levels by binding to ODC and targeting it for degradation, as well as by inhibiting polyamine uptake. Antizyme inhibitors function as positive regulators of polyamine levels by sequestering antizymes and neutralizing their effect. This gene encodes one of two copies of antizyme inhibitor 1 in zebrafish. [provided by RefSeq, May 2015].

mRNA and Protein(s)

mRNA	Protein	Name
XM_005158442.3	XP_005158499.1	antizyme inhibitor 1 isoform X1
NM_001017568.3	NP_001017568.2	antizyme inhibitor 1
XM_005158442.2	XP_005158499.1	antizyme inhibitor 1 isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Identification of target genes downstream of semaphorin6A/PlexinA2 signaling in zebrafish.
Emerson SE, St Clair RM, Waldron AL, Bruno SR, Duong A, Driscoll HE, Ballif BA, McFarlane S, Ebert AM
Developmental dynamics : an official publication of the American Association of Anatomists246(7)539-549(2017 Jul)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Evolution of gene function and regulatory control after whole-genome duplication: comparative analyses in vertebrates.
Kassahn KS, Dang VT, Wilkins SJ, Perkins AC, Ragan MA
Genome research19(8)1404-18(2009 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AZIN1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AZIN1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa01855	NM_001017568.3 Latest version!	Danio rerio antizyme inhibitor 1a (azin1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ODa01855	XM_005158442.3 Latest version!	Danio rerio antizyme inhibitor 1a (azin1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
ODa01855	XM_005158442.2	Danio rerio antizyme inhibitor 1a (azin1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AZIN1A ( NM_001017568.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001017568.2 Danio rerio antizyme inhibitor 1a (azin1a), mRNA.

Price & Availability↑

CloneID	ODa01855
Clone ID Related Accession (Same CDS sequence)	XM_005158442.3 , NM_001017568.3 , XM_005158442.2
Accession Version	NM_001017568.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	antizyme inhibitor 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CR524823.18. On Sep 6, 2014 this sequence version replaced NM_001017568.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: GFIL01002087.1, GDQH01013143.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTATGA AGGGATCTCT GGATGAACTG CATTATTCTG TTGAGCTGCT GGAGGGAAGC 
GCAGCTCTGC GAGATGTGAT CGATAAACAC ATCTATGATC AGACTTTTGC ACAGAAAAGT 
GCGTTTTTTG CTGCTGATCT GGGGGTGATC GTGCAGCAGC AGGCTCGCTG GAGAAATAAA 
ATGGACCAAA TTCGGCCTTT CTACACCGTT AAGAGCAACA GCAGCCCTGT AGTTGTTGAA 
CTGCTGGCTT CTCTCGGAAC TGGCTTCATT TGTTCAAACA AGCATGAGCT GGACTTGGTT 
AAGGGCTTTG GCGTCTCATC TCAGGACATT ATTCTGGGCG GCACGTGCAA ACAGCTCTCC 
CATATCAAAT ATGCAGCCAA ACACAACATC CCGCTCCTGG TTTGTGACAA TGAGGCAGAG 
ATGAGGAAGA TCGCACGATG TCATCCTAAA GCAAAAGTGT TGCTGCTGTT GACGTCGGAG 
AGCTGCTGTG AGAAAGAGGA GATGGCCATA CCGTTCGGCT CCACACTGAA GGACTGCAGG 
CGTCTGCTGG AGTGTGCTGC TGGAGAACTC AATCTGCAAG TCACTGGAGT CAAATTCAAC 
ATTCCCAGCT GCTGTCAGGA AGTAGAGAAG TTCTCTCGTG CTGTTTCTGA GGCCCGCTGT 
GTCTTTGATA TGGGGGAAGA GCTCGGCTTC GAGATGAATA TCCTGGACAT CGGAGGAGGT 
TTTGATGGAA GTGAGGAACA GTTAGACAAG GTTAACCAGA TGCTGAAGCC CATGCTGGAC 
ATGTATTTTC CTCGGTCGAC TGGCTTGTCA ATCATTGCTG AACCTGGAGC TTATTACGTT 
TCTTCTGCCT TCACGCTGGC CGTGAATATA ATCGATAAGA AGACGGTGGC TCGGGACTGC 
AGTGCACAGC CACACGATGC ACTGTCTGCA AACGATGAGC CAGAGTTTCT CTACCACATA 
AATGATGGTG TTTATGGATC TTTTGCCAGT AAACTGCTGT GTGAAGATTC AATAGCAACA 
CCTTTGGCAC ACAAACATGT GAGCGCAGAC GAGCCGCTGT TCTCCAGCAG TCTGTGGGGT 
CCCTCTGAAG ATGATCTGGA TCTGGTGATG GAGCGATGTC TGCTGCCCGA GCTCAGTGTT 
GGAGACTGGC TGCTGTTCAG AAACGCAGGA GCCAGCGCTC TGGGGTCCGC ATTCAGTCAC 
GGAGACCAAC ACAAACCGCC CGTCTTCTAC AGCATCACTG AATACGACTG GCAAGAGATC 
CACAATAGTG GCGTGACTGT GGATGTGAGG ATGAAGAACT TCTCTCTGGT ACCTTGTTGC 
CTGCAACCAA ACATGTCCGG GGCATCCATT TCTACTCCTG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001017568.2
CDS	393..1757
Translation	MSMKGSLDELHYSVELLEGSAALRDVIDKHIYDQTFAQKSAFFAADLGVIVQQQARWRNKMDQIRPFYTVKSNSSPVVVELLASLGTGFICSNKHELDLVKGFGVSSQDIILGGTCKQLSHIKYAAKHNIPLLVCDNEAEMRKIARCHPKAKVLLLLTSESCCEKEEMAIPFGSTLKDCRRLLECAAGELNLQVTGVKFNIPSCCQEVEKFSRAVSEARCVFDMGEELGFEMNILDIGGGFDGSEEQLDKVNQMLKPMLDMYFPRSTGLSIIAEPGAYYVSSAFTLAVNIIDKKTVARDCSAQPHDALSANDEPEFLYHINDGVYGSFASKLLCEDSIATPLAHKHVSADEPLFSSSLWGPSEDDLDLVMERCLLPELSVGDWLLFRNAGASALGSAFSHGDQHKPPVFYSITEYDWQEIHNSGVTVDVRMKNFSLVPCCLQPNMSGASISTPA

Target ORF information:

RefSeq Version	NM_001017568.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio antizyme inhibitor 1a (azin1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001017568.3

ORF Insert Sequence:

ATGAGTATGA AGGGATCTCT GGATGAACTG CATTATTCTG TTGAGCTGCT GGAGGGAAGC 
GCAGCTCTGC GAGATGTGAT CGATAAACAC ATCTATGATC AGACTTTTGC ACAGAAAAGT 
GCGTTTTTTG CTGCTGATCT GGGGGTGATC GTGCAGCAGC AGGCTCGCTG GAGAAATAAA 
ATGGACCAAA TTCGGCCTTT CTACACCGTT AAGAGCAACA GCAGCCCTGT AGTTGTTGAA 
CTGCTGGCTT CTCTCGGAAC TGGCTTCATT TGTTCAAACA AGCATGAGCT GGACTTGGTT 
AAGGGCTTTG GCGTCTCATC TCAGGACATT ATTCTGGGCG GCACGTGCAA ACAGCTCTCC 
CATATCAAAT ATGCAGCCAA ACACAACATC CCGCTCCTGG TTTGTGACAA TGAGGCAGAG 
ATGAGGAAGA TCGCACGATG TCATCCTAAA GCAAAAGTGT TGCTGCTGTT GACGTCGGAG 
AGCTGCTGTG AGAAAGAGGA GATGGCCATA CCGTTCGGCT CCACACTGAA GGACTGCAGG 
CGTCTGCTGG AGTGTGCTGC TGGAGAACTC AATCTGCAAG TCACTGGAGT CAAATTCAAC 
ATTCCCAGCT GCTGTCAGGA AGTAGAGAAG TTCTCTCGTG CTGTTTCTGA GGCCCGCTGT 
GTCTTTGATA TGGGGGAAGA GCTCGGCTTC GAGATGAATA TCCTGGACAT CGGAGGAGGT 
TTTGATGGAA GTGAGGAACA GTTAGACAAG GTTAACCAGA TGCTGAAGCC CATGCTGGAC 
ATGTATTTTC CTCGGTCGAC TGGCTTGTCA ATCATTGCTG AACCTGGAGC TTATTACGTT 
TCTTCTGCCT TCACGCTGGC CGTGAATATA ATCGATAAGA AGACGGTGGC TCGGGACTGC 
AGTGCACAGC CACACGATGC ACTGTCTGCA AACGATGAGC CAGAGTTTCT CTACCACATA 
AATGATGGTG TTTATGGATC TTTTGCCAGT AAACTGCTGT GTGAAGATTC AATAGCAACA 
CCTTTGGCAC ACAAACATGT GAGCGCAGAC GAGCCGCTGT TCTCCAGCAG TCTGTGGGGT 
CCCTCTGAAG ATGATCTGGA TCTGGTGATG GAGCGATGTC TGCTGCCCGA GCTCAGTGTT 
GGAGACTGGC TGCTGTTCAG AAACGCAGGA GCCAGCGCTC TGGGGTCCGC ATTCAGTCAC 
GGAGACCAAC ACAAACCGCC CGTCTTCTAC AGCATCACTG AATACGACTG GCAAGAGATC 
CACAATAGTG GCGTGACTGT GGATGTGAGG ATGAAGAACT TCTCTCTGGT ACCTTGTTGC 
CTGCAACCAA ACATGTCCGG GGCATCCATT TCTACTCCTG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AZIN1A ( XM_005158442.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005158499.1 Danio rerio antizyme inhibitor 1a (azin1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa01855
Clone ID Related Accession (Same CDS sequence)	XM_005158442.3 , NM_001017568.3 , XM_005158442.2
Accession Version	XM_005158442.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	antizyme inhibitor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005158442.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTATGA AGGGATCTCT GGATGAACTG CATTATTCTG TTGAGCTGCT GGAGGGAAGC 
GCAGCTCTGC GAGATGTGAT CGATAAACAC ATCTATGATC AGACTTTTGC ACAGAAAAGT 
GCGTTTTTTG CTGCTGATCT GGGGGTGATC GTGCAGCAGC AGGCTCGCTG GAGAAATAAA 
ATGGACCAAA TTCGGCCTTT CTACACCGTT AAGAGCAACA GCAGCCCTGT AGTTGTTGAA 
CTGCTGGCTT CTCTCGGAAC TGGCTTCATT TGTTCAAACA AGCATGAGCT GGACTTGGTT 
AAGGGCTTTG GCGTCTCATC TCAGGACATT ATTCTGGGCG GCACGTGCAA ACAGCTCTCC 
CATATCAAAT ATGCAGCCAA ACACAACATC CCGCTCCTGG TTTGTGACAA TGAGGCAGAG 
ATGAGGAAGA TCGCACGATG TCATCCTAAA GCAAAAGTGT TGCTGCTGTT GACGTCGGAG 
AGCTGCTGTG AGAAAGAGGA GATGGCCATA CCGTTCGGCT CCACACTGAA GGACTGCAGG 
CGTCTGCTGG AGTGTGCTGC TGGAGAACTC AATCTGCAAG TCACTGGAGT CAAATTCAAC 
ATTCCCAGCT GCTGTCAGGA AGTAGAGAAG TTCTCTCGTG CTGTTTCTGA GGCCCGCTGT 
GTCTTTGATA TGGGGGAAGA GCTCGGCTTC GAGATGAATA TCCTGGACAT CGGAGGAGGT 
TTTGATGGAA GTGAGGAACA GTTAGACAAG GTTAACCAGA TGCTGAAGCC CATGCTGGAC 
ATGTATTTTC CTCGGTCGAC TGGCTTGTCA ATCATTGCTG AACCTGGAGC TTATTACGTT 
TCTTCTGCCT TCACGCTGGC CGTGAATATA ATCGATAAGA AGACGGTGGC TCGGGACTGC 
AGTGCACAGC CACACGATGC ACTGTCTGCA AACGATGAGC CAGAGTTTCT CTACCACATA 
AATGATGGTG TTTATGGATC TTTTGCCAGT AAACTGCTGT GTGAAGATTC AATAGCAACA 
CCTTTGGCAC ACAAACATGT GAGCGCAGAC GAGCCGCTGT TCTCCAGCAG TCTGTGGGGT 
CCCTCTGAAG ATGATCTGGA TCTGGTGATG GAGCGATGTC TGCTGCCCGA GCTCAGTGTT 
GGAGACTGGC TGCTGTTCAG AAACGCAGGA GCCAGCGCTC TGGGGTCCGC ATTCAGTCAC 
GGAGACCAAC ACAAACCGCC CGTCTTCTAC AGCATCACTG AATACGACTG GCAAGAGATC 
CACAATAGTG GCGTGACTGT GGATGTGAGG ATGAAGAACT TCTCTCTGGT ACCTTGTTGC 
CTGCAACCAA ACATGTCCGG GGCATCCATT TCTACTCCTG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158499.1
CDS	365..1729
Translation	MSMKGSLDELHYSVELLEGSAALRDVIDKHIYDQTFAQKSAFFAADLGVIVQQQARWRNKMDQIRPFYTVKSNSSPVVVELLASLGTGFICSNKHELDLVKGFGVSSQDIILGGTCKQLSHIKYAAKHNIPLLVCDNEAEMRKIARCHPKAKVLLLLTSESCCEKEEMAIPFGSTLKDCRRLLECAAGELNLQVTGVKFNIPSCCQEVEKFSRAVSEARCVFDMGEELGFEMNILDIGGGFDGSEEQLDKVNQMLKPMLDMYFPRSTGLSIIAEPGAYYVSSAFTLAVNIIDKKTVARDCSAQPHDALSANDEPEFLYHINDGVYGSFASKLLCEDSIATPLAHKHVSADEPLFSSSLWGPSEDDLDLVMERCLLPELSVGDWLLFRNAGASALGSAFSHGDQHKPPVFYSITEYDWQEIHNSGVTVDVRMKNFSLVPCCLQPNMSGASISTPA

Target ORF information:

RefSeq Version	XM_005158442.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio antizyme inhibitor 1a (azin1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158442.3

ORF Insert Sequence:

ATGAGTATGA AGGGATCTCT GGATGAACTG CATTATTCTG TTGAGCTGCT GGAGGGAAGC 
GCAGCTCTGC GAGATGTGAT CGATAAACAC ATCTATGATC AGACTTTTGC ACAGAAAAGT 
GCGTTTTTTG CTGCTGATCT GGGGGTGATC GTGCAGCAGC AGGCTCGCTG GAGAAATAAA 
ATGGACCAAA TTCGGCCTTT CTACACCGTT AAGAGCAACA GCAGCCCTGT AGTTGTTGAA 
CTGCTGGCTT CTCTCGGAAC TGGCTTCATT TGTTCAAACA AGCATGAGCT GGACTTGGTT 
AAGGGCTTTG GCGTCTCATC TCAGGACATT ATTCTGGGCG GCACGTGCAA ACAGCTCTCC 
CATATCAAAT ATGCAGCCAA ACACAACATC CCGCTCCTGG TTTGTGACAA TGAGGCAGAG 
ATGAGGAAGA TCGCACGATG TCATCCTAAA GCAAAAGTGT TGCTGCTGTT GACGTCGGAG 
AGCTGCTGTG AGAAAGAGGA GATGGCCATA CCGTTCGGCT CCACACTGAA GGACTGCAGG 
CGTCTGCTGG AGTGTGCTGC TGGAGAACTC AATCTGCAAG TCACTGGAGT CAAATTCAAC 
ATTCCCAGCT GCTGTCAGGA AGTAGAGAAG TTCTCTCGTG CTGTTTCTGA GGCCCGCTGT 
GTCTTTGATA TGGGGGAAGA GCTCGGCTTC GAGATGAATA TCCTGGACAT CGGAGGAGGT 
TTTGATGGAA GTGAGGAACA GTTAGACAAG GTTAACCAGA TGCTGAAGCC CATGCTGGAC 
ATGTATTTTC CTCGGTCGAC TGGCTTGTCA ATCATTGCTG AACCTGGAGC TTATTACGTT 
TCTTCTGCCT TCACGCTGGC CGTGAATATA ATCGATAAGA AGACGGTGGC TCGGGACTGC 
AGTGCACAGC CACACGATGC ACTGTCTGCA AACGATGAGC CAGAGTTTCT CTACCACATA 
AATGATGGTG TTTATGGATC TTTTGCCAGT AAACTGCTGT GTGAAGATTC AATAGCAACA 
CCTTTGGCAC ACAAACATGT GAGCGCAGAC GAGCCGCTGT TCTCCAGCAG TCTGTGGGGT 
CCCTCTGAAG ATGATCTGGA TCTGGTGATG GAGCGATGTC TGCTGCCCGA GCTCAGTGTT 
GGAGACTGGC TGCTGTTCAG AAACGCAGGA GCCAGCGCTC TGGGGTCCGC ATTCAGTCAC 
GGAGACCAAC ACAAACCGCC CGTCTTCTAC AGCATCACTG AATACGACTG GCAAGAGATC 
CACAATAGTG GCGTGACTGT GGATGTGAGG ATGAAGAACT TCTCTCTGGT ACCTTGTTGC 
CTGCAACCAA ACATGTCCGG GGCATCCATT TCTACTCCTG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AZIN1A ( XM_005158442.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005158499.1 Danio rerio antizyme inhibitor 1a (azin1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa01855
Clone ID Related Accession (Same CDS sequence)	XM_005158442.3 , NM_001017568.3 , XM_005158442.2
Accession Version	XM_005158442.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	antizyme inhibitor 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2014 this sequence version replaced XM_005158442.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTATGA AGGGATCTCT GGATGAACTG CATTATTCTG TTGAGCTGCT GGAGGGAAGC 
GCAGCTCTGC GAGATGTGAT CGATAAACAC ATCTATGATC AGACTTTTGC ACAGAAAAGT 
GCGTTTTTTG CTGCTGATCT GGGGGTGATC GTGCAGCAGC AGGCTCGCTG GAGAAATAAA 
ATGGACCAAA TTCGGCCTTT CTACACCGTT AAGAGCAACA GCAGCCCTGT AGTTGTTGAA 
CTGCTGGCTT CTCTCGGAAC TGGCTTCATT TGTTCAAACA AGCATGAGCT GGACTTGGTT 
AAGGGCTTTG GCGTCTCATC TCAGGACATT ATTCTGGGCG GCACGTGCAA ACAGCTCTCC 
CATATCAAAT ATGCAGCCAA ACACAACATC CCGCTCCTGG TTTGTGACAA TGAGGCAGAG 
ATGAGGAAGA TCGCACGATG TCATCCTAAA GCAAAAGTGT TGCTGCTGTT GACGTCGGAG 
AGCTGCTGTG AGAAAGAGGA GATGGCCATA CCGTTCGGCT CCACACTGAA GGACTGCAGG 
CGTCTGCTGG AGTGTGCTGC TGGAGAACTC AATCTGCAAG TCACTGGAGT CAAATTCAAC 
ATTCCCAGCT GCTGTCAGGA AGTAGAGAAG TTCTCTCGTG CTGTTTCTGA GGCCCGCTGT 
GTCTTTGATA TGGGGGAAGA GCTCGGCTTC GAGATGAATA TCCTGGACAT CGGAGGAGGT 
TTTGATGGAA GTGAGGAACA GTTAGACAAG GTTAACCAGA TGCTGAAGCC CATGCTGGAC 
ATGTATTTTC CTCGGTCGAC TGGCTTGTCA ATCATTGCTG AACCTGGAGC TTATTACGTT 
TCTTCTGCCT TCACGCTGGC CGTGAATATA ATCGATAAGA AGACGGTGGC TCGGGACTGC 
AGTGCACAGC CACACGATGC ACTGTCTGCA AACGATGAGC CAGAGTTTCT CTACCACATA 
AATGATGGTG TTTATGGATC TTTTGCCAGT AAACTGCTGT GTGAAGATTC AATAGCAACA 
CCTTTGGCAC ACAAACATGT GAGCGCAGAC GAGCCGCTGT TCTCCAGCAG TCTGTGGGGT 
CCCTCTGAAG ATGATCTGGA TCTGGTGATG GAGCGATGTC TGCTGCCCGA GCTCAGTGTT 
GGAGACTGGC TGCTGTTCAG AAACGCAGGA GCCAGCGCTC TGGGGTCCGC ATTCAGTCAC 
GGAGACCAAC ACAAACCGCC CGTCTTCTAC AGCATCACTG AATACGACTG GCAAGAGATC 
CACAATAGTG GCGTGACTGT GGATGTGAGG ATGAAGAACT TCTCTCTGGT ACCTTGTTGC 
CTGCAACCAA ACATGTCCGG GGCATCCATT TCTACTCCTG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158499.1
CDS	363..1727
Translation	MSMKGSLDELHYSVELLEGSAALRDVIDKHIYDQTFAQKSAFFAADLGVIVQQQARWRNKMDQIRPFYTVKSNSSPVVVELLASLGTGFICSNKHELDLVKGFGVSSQDIILGGTCKQLSHIKYAAKHNIPLLVCDNEAEMRKIARCHPKAKVLLLLTSESCCEKEEMAIPFGSTLKDCRRLLECAAGELNLQVTGVKFNIPSCCQEVEKFSRAVSEARCVFDMGEELGFEMNILDIGGGFDGSEEQLDKVNQMLKPMLDMYFPRSTGLSIIAEPGAYYVSSAFTLAVNIIDKKTVARDCSAQPHDALSANDEPEFLYHINDGVYGSFASKLLCEDSIATPLAHKHVSADEPLFSSSLWGPSEDDLDLVMERCLLPELSVGDWLLFRNAGASALGSAFSHGDQHKPPVFYSITEYDWQEIHNSGVTVDVRMKNFSLVPCCLQPNMSGASISTPA

Target ORF information:

RefSeq Version	XM_005158442.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio antizyme inhibitor 1a (azin1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158442.2

ORF Insert Sequence:

ATGAGTATGA AGGGATCTCT GGATGAACTG CATTATTCTG TTGAGCTGCT GGAGGGAAGC 
GCAGCTCTGC GAGATGTGAT CGATAAACAC ATCTATGATC AGACTTTTGC ACAGAAAAGT 
GCGTTTTTTG CTGCTGATCT GGGGGTGATC GTGCAGCAGC AGGCTCGCTG GAGAAATAAA 
ATGGACCAAA TTCGGCCTTT CTACACCGTT AAGAGCAACA GCAGCCCTGT AGTTGTTGAA 
CTGCTGGCTT CTCTCGGAAC TGGCTTCATT TGTTCAAACA AGCATGAGCT GGACTTGGTT 
AAGGGCTTTG GCGTCTCATC TCAGGACATT ATTCTGGGCG GCACGTGCAA ACAGCTCTCC 
CATATCAAAT ATGCAGCCAA ACACAACATC CCGCTCCTGG TTTGTGACAA TGAGGCAGAG 
ATGAGGAAGA TCGCACGATG TCATCCTAAA GCAAAAGTGT TGCTGCTGTT GACGTCGGAG 
AGCTGCTGTG AGAAAGAGGA GATGGCCATA CCGTTCGGCT CCACACTGAA GGACTGCAGG 
CGTCTGCTGG AGTGTGCTGC TGGAGAACTC AATCTGCAAG TCACTGGAGT CAAATTCAAC 
ATTCCCAGCT GCTGTCAGGA AGTAGAGAAG TTCTCTCGTG CTGTTTCTGA GGCCCGCTGT 
GTCTTTGATA TGGGGGAAGA GCTCGGCTTC GAGATGAATA TCCTGGACAT CGGAGGAGGT 
TTTGATGGAA GTGAGGAACA GTTAGACAAG GTTAACCAGA TGCTGAAGCC CATGCTGGAC 
ATGTATTTTC CTCGGTCGAC TGGCTTGTCA ATCATTGCTG AACCTGGAGC TTATTACGTT 
TCTTCTGCCT TCACGCTGGC CGTGAATATA ATCGATAAGA AGACGGTGGC TCGGGACTGC 
AGTGCACAGC CACACGATGC ACTGTCTGCA AACGATGAGC CAGAGTTTCT CTACCACATA 
AATGATGGTG TTTATGGATC TTTTGCCAGT AAACTGCTGT GTGAAGATTC AATAGCAACA 
CCTTTGGCAC ACAAACATGT GAGCGCAGAC GAGCCGCTGT TCTCCAGCAG TCTGTGGGGT 
CCCTCTGAAG ATGATCTGGA TCTGGTGATG GAGCGATGTC TGCTGCCCGA GCTCAGTGTT 
GGAGACTGGC TGCTGTTCAG AAACGCAGGA GCCAGCGCTC TGGGGTCCGC ATTCAGTCAC 
GGAGACCAAC ACAAACCGCC CGTCTTCTAC AGCATCACTG AATACGACTG GCAAGAGATC 
CACAATAGTG GCGTGACTGT GGATGTGAGG ATGAAGAACT TCTCTCTGGT ACCTTGTTGC 
CTGCAACCAA ACATGTCCGG GGCATCCATT TCTACTCCTG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of target genes downstream of semaphorin6A/PlexinA2 signaling in zebrafish.
Developmental dynamics : an official publication of the American Association of Anatomists246(7)539-549(2017 Jul)
Emerson SE,St Clair RM,Waldron AL,Bruno SR,Duong A,Driscoll HE,Ballif BA,McFarlane S,Ebert AM

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Evolution of gene function and regulatory control after whole-genome duplication: comparative analyses in vertebrates.
Genome research19(8)1404-18(2009 Aug)
Kassahn KS,Dang VT,Wilkins SJ,Perkins AC,Ragan MA

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AZIN1A cDNA ORF clone, Danio rerio(Zebrafish)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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