hif1al2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	hif1al2
Entrez Gene ID	497283
Full Name	hypoxia-inducible factor 1, alpha subunit, like 2
Synonyms	hif1al,zgc:112119
General protein information	Preferred Names hypoxia-inducible factor 1, alpha subunit, like 2 Names hypoxia-inducible factor 1, alpha subunit, like 2 HIF3B Hif2a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005161350.4	XP_005161407.1	hypoxia-inducible factor 1, alpha subunit, like 2 isoform X1
XM_005161350.3	XP_005161407.1	hypoxia-inducible factor 1, alpha subunit, like 2 isoform X1
XM_021468817.1	XP_021324492.1	hypoxia-inducible factor 1, alpha subunit, like 2 isoform X2
NM_001012371.2	NP_001012371.2	hypoxia-inducible factor 1, alpha subunit, like 2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Age-dependent increase of oxidative stress regulates microRNA-29 family preserving cardiac health.
Heid J, Cencioni C, Ripa R, Baumgart M, Atlante S, Milano G, Scopece A, Kuenne C, Guenther S, Azzimato V, Farsetti A, Rossi G, Braun T, Pompilio G, Martelli F, Zeiher AM, Cellerino A, Gaetano C, Spallotta F
Scientific reports7(1)16839(2017 Dec)

Carbon nanopowder acts as a Trojan-horse for benzo(?)pyrene in Danio rerio embryos.
Binelli A, Del Giacco L, Santo N, Bini L, Magni S, Parolini M, Madaschi L, Ghilardi A, Maggioni D, Ascagni M, Armini A, Prosperi L, Landi C, La Porta C, Della Torre C
Nanotoxicology11(3)371-381(2017 Apr)

Foxa2 and Hif1ab regulate maturation of intestinal goblet cells by modulating agr2 expression in zebrafish embryos.
Lai YR, Lu YF, Lien HW, Huang CJ, Hwang SP
The Biochemical journal473(14)2205-18(2016 07)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Phenotype anchoring in zebrafish reveals a potential role for matrix metalloproteinases (MMPs) in tamoxifen's effects on skin epithelium.
Bugel SM, Wehmas LC, La Du JK, Tanguay RL
Toxicology and applied pharmacology29631-41(2016 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Prominent changes were seen for Hif-2alpha expression, which significantly increased after the first day of exercise and then decreased down to values significantly below control values.
Title: HIF signaling and overall gene expression changes during hypoxia and prolonged exercise differ considerably.

The following hif1al2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hif1al2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa25967	NM_001012371.2 Latest version!	Danio rerio hypoxia inducible factor 1 subunit alpha, like 2 (hif1al2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa25967	XM_005161350.4 Latest version!	Danio rerio hypoxia-inducible factor 1, alpha subunit, like 2 (hif1al2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa150883	XM_021468817.1 Latest version!	Danio rerio hypoxia-inducible factor 1, alpha subunit, like 2 (hif1al2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
ODa25967	XM_005161350.3	Danio rerio hypoxia-inducible factor 1, alpha subunit, like 2 (hif1al2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hif1al2 ( NM_001012371.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001012371.2 Danio rerio hypoxia inducible factor 1 subunit alpha, like 2 (hif1al2), mRNA.

Price & Availability↑

CloneID	ODa25967
Clone ID Related Accession (Same CDS sequence)	XM_005161350.4 , XM_005161350.3 , NM_001012371.2
Accession Version	NM_001012371.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	hypoxia-inducible factor 1, alpha subunit, like 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX530031.5, DT057411.1 and CK030317.1. On Jun 25, 2015 this sequence version replaced NM_001012371.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the reference strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GFIL01012518.1, BC093209.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGACTTCAA TGAATTTGGA AACAGGAGGA GAAGACTGCC ACGGAAAAGC TAGAAGAAAG 
CAAACATTCA TCGAGCAGAA GAAAAGGAGA GTCTGCACAG AATGGAGAAA AACATGTTCC 
CGTTTGGCAG CACAGAGCAG GAGGAAGAAG GAGAGTCAAC TGTTCAAAGA GTTAACTGCT 
CTTCTGCCTC TGGACTTGAG TATGGACGGT CAGCGAGACA AAGCTTCGGT AATACGTCTC 
ACAATTGCTT ACCTGCACCT GAGGGGTCTT TTGGACACCA CAGACTCCTT GATGAAACGT 
CCTTCACCAG GAGTGTCGGA TAAATCTCAT GAGAGGGAGT TGCTAGATTC TGCCCTCGGA 
GGGTTTGTGG TTTTGTTGTC TTTGAATGGC AAGGTCATCT TCGCCACTAA GGGCTTAACT 
ACCCACACAG GAATTAACCA GATGGATCTG ATCGGAAGGA GCTTGCTTGA GTTCTTGCAC 
CCATGCGATC AGATGGAGGT CAAAGACATC CTCACGAGAC TGATTGGGAA CCAAGAACCA 
CAAAAATGTG AAGTGTTCCT CAGGATAAAA AAGGCAATGA ACACGAAACT GACTGGTTGG 
AAAATTATTC AGTGTTCTGG AAAGAAAAGG TCATCTGCTA CACCTGGATC CAGTTGTCTG 
GTTCTTGAGT GCAGGGTTCT GCCCATTCAG GAGATCACAG AAGTGGATGA AGCATTGAAT 
GCATGTACAT TTATGAGCGT TCACAGCCCT GACATGACGT TCACCTACTG CCACTCAAGG 
GTCGTGAAAT TGATCGGCTT TCGAGACACA GATCTGTTGG GTCAATCTGT ATACCAGTAC 
TATCATCCAT CTGACTGCCA GCAAATCCGC AAGGCACACA TTTGCTTGCT CTCCAAGGGT 
CAAGTGTCGA CCGGGAAATA CCGGCTGCTG CACAGATATG GAGGTTATGT GTGGGCAGAA 
ACAGACGCCT CACTGGTGTG CAACAGTCAG ACTGGTGTGC CAGAGAGTGT CGTCTGCATC 
AATTACATTC TAAGTGAAGT AGAGCAGCCC AATTTGCCAT TTTTACTGGA GAAGACAGAA 
CAACTTCTGA AACCAACATC TAGCTCCTAT TGTCCAGAGA CAGAACCTGC CCCTTTTCCG 
CCATCTAGAG GCCAGGAGGG TGAGAAAGAT CTCAAGGAAT CGAACGAACA CAACAGTTTT 
ATAACACATC TGAAACAAAG AGATGGTGAC ATACAGGAAG TTGAATGTAC AGAGATCCCA 
CATAACACAG AAGGGTGTTA TGATTTCAAT TGTGATCAGT GTGAGTTAGA CTTGGACAGT 
CTTGCTCCAT ACATTCCCAT GCACGGAGAG GACTTCCTGC TAACTCCCAT TATAGACAGG 
GCGATGGACA TGCCAGAATT AAACACGTGT AGACCATCAT GTAGCAGTCC ATCTCAAAAC 
CTGGATTCTT CAGAGGGAAC AACCGTTCTG CCAGTGAAGA GTCTTAATTC TACCCAACAA 
AGTGTCAGTA ATTCTTTTCA GACAAAAAGA ATCAGCGATA CTAACCTGAT ATCAACCAAG 
CAGTGGAGTG ACAGCTTTGA CCTTGTCAAC AAAGTTTCAA AGGTTTATGA TTACGATCCT 
AAACAACATA ATTATCAACT ATTTTCATCT GAAACCAAGG CAAATCATTG GGTGGAGGAG 
GCTCTGAAAT TTCCACAACC CAACAGAGTT CATTGGAACA TCGATGGATC AACACCACCA 
TGGATATGTG ACGGACACTT GAATCATCTT GAACCGAAGT TCAGATCACT TCCAGAGAAA 
ACCAATTGTC CTCCTCAAAA GAGAAGACAC CAACATGAAG TAAAAACAGC CGCGGCACTT 
TTGACTCTGC CTGTGTTGAG CGGATGGGAG TGTGAAGTCA ACGCTCCTCT CGAACACACA 
TCATATTTAC TCCAAGGGAC AGAGATTATG GCCGTTCTGG ACCAAGTAGC CTCCAGAGTG 
CCTTGGTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001012371.2
CDS	88..2079
Translation	MTSMNLETGGEDCHGKARRKQTFIEQKKRRVCTEWRKTCSRLAAQSRRKKESQLFKELTALLPLDLSMDGQRDKASVIRLTIAYLHLRGLLDTTDSLMKRPSPGVSDKSHERELLDSALGGFVVLLSLNGKVIFATKGLTTHTGINQMDLIGRSLLEFLHPCDQMEVKDILTRLIGNQEPQKCEVFLRIKKAMNTKLTGWKIIQCSGKKRSSATPGSSCLVLECRVLPIQEITEVDEALNACTFMSVHSPDMTFTYCHSRVVKLIGFRDTDLLGQSVYQYYHPSDCQQIRKAHICLLSKGQVSTGKYRLLHRYGGYVWAETDASLVCNSQTGVPESVVCINYILSEVEQPNLPFLLEKTEQLLKPTSSSYCPETEPAPFPPSRGQEGEKDLKESNEHNSFITHLKQRDGDIQEVECTEIPHNTEGCYDFNCDQCELDLDSLAPYIPMHGEDFLLTPIIDRAMDMPELNTCRPSCSSPSQNLDSSEGTTVLPVKSLNSTQQSVSNSFQTKRISDTNLISTKQWSDSFDLVNKVSKVYDYDPKQHNYQLFSSETKANHWVEEALKFPQPNRVHWNIDGSTPPWICDGHLNHLEPKFRSLPEKTNCPPQKRRHQHEVKTAAALLTLPVLSGWECEVNAPLEHTSYLLQGTEIMAVLDQVASRVPWC

Target ORF information:

RefSeq Version	NM_001012371.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio hypoxia inducible factor 1 subunit alpha, like 2 (hif1al2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001012371.2

ORF Insert Sequence:

ATGACTTCAA TGAATTTGGA AACAGGAGGA GAAGACTGCC ACGGAAAAGC TAGAAGAAAG 
CAAACATTCA TCGAGCAGAA GAAAAGGAGA GTCTGCACAG AATGGAGAAA AACATGTTCC 
CGTTTGGCAG CACAGAGCAG GAGGAAGAAG GAGAGTCAAC TGTTCAAAGA GTTAACTGCT 
CTTCTGCCTC TGGACTTGAG TATGGACGGT CAGCGAGACA AAGCTTCGGT AATACGTCTC 
ACAATTGCTT ACCTGCACCT GAGGGGTCTT TTGGACACCA CAGACTCCTT GATGAAACGT 
CCTTCACCAG GAGTGTCGGA TAAATCTCAT GAGAGGGAGT TGCTAGATTC TGCCCTCGGA 
GGGTTTGTGG TTTTGTTGTC TTTGAATGGC AAGGTCATCT TCGCCACTAA GGGCTTAACT 
ACCCACACAG GAATTAACCA GATGGATCTG ATCGGAAGGA GCTTGCTTGA GTTCTTGCAC 
CCATGCGATC AGATGGAGGT CAAAGACATC CTCACGAGAC TGATTGGGAA CCAAGAACCA 
CAAAAATGTG AAGTGTTCCT CAGGATAAAA AAGGCAATGA ACACGAAACT GACTGGTTGG 
AAAATTATTC AGTGTTCTGG AAAGAAAAGG TCATCTGCTA CACCTGGATC CAGTTGTCTG 
GTTCTTGAGT GCAGGGTTCT GCCCATTCAG GAGATCACAG AAGTGGATGA AGCATTGAAT 
GCATGTACAT TTATGAGCGT TCACAGCCCT GACATGACGT TCACCTACTG CCACTCAAGG 
GTCGTGAAAT TGATCGGCTT TCGAGACACA GATCTGTTGG GTCAATCTGT ATACCAGTAC 
TATCATCCAT CTGACTGCCA GCAAATCCGC AAGGCACACA TTTGCTTGCT CTCCAAGGGT 
CAAGTGTCGA CCGGGAAATA CCGGCTGCTG CACAGATATG GAGGTTATGT GTGGGCAGAA 
ACAGACGCCT CACTGGTGTG CAACAGTCAG ACTGGTGTGC CAGAGAGTGT CGTCTGCATC 
AATTACATTC TAAGTGAAGT AGAGCAGCCC AATTTGCCAT TTTTACTGGA GAAGACAGAA 
CAACTTCTGA AACCAACATC TAGCTCCTAT TGTCCAGAGA CAGAACCTGC CCCTTTTCCG 
CCATCTAGAG GCCAGGAGGG TGAGAAAGAT CTCAAGGAAT CGAACGAACA CAACAGTTTT 
ATAACACATC TGAAACAAAG AGATGGTGAC ATACAGGAAG TTGAATGTAC AGAGATCCCA 
CATAACACAG AAGGGTGTTA TGATTTCAAT TGTGATCAGT GTGAGTTAGA CTTGGACAGT 
CTTGCTCCAT ACATTCCCAT GCACGGAGAG GACTTCCTGC TAACTCCCAT TATAGACAGG 
GCGATGGACA TGCCAGAATT AAACACGTGT AGACCATCAT GTAGCAGTCC ATCTCAAAAC 
CTGGATTCTT CAGAGGGAAC AACCGTTCTG CCAGTGAAGA GTCTTAATTC TACCCAACAA 
AGTGTCAGTA ATTCTTTTCA GACAAAAAGA ATCAGCGATA CTAACCTGAT ATCAACCAAG 
CAGTGGAGTG ACAGCTTTGA CCTTGTCAAC AAAGTTTCAA AGGTTTATGA TTACGATCCT 
AAACAACATA ATTATCAACT ATTTTCATCT GAAACCAAGG CAAATCATTG GGTGGAGGAG 
GCTCTGAAAT TTCCACAACC CAACAGAGTT CATTGGAACA TCGATGGATC AACACCACCA 
TGGATATGTG ACGGACACTT GAATCATCTT GAACCGAAGT TCAGATCACT TCCAGAGAAA 
ACCAATTGTC CTCCTCAAAA GAGAAGACAC CAACATGAAG TAAAAACAGC CGCGGCACTT 
TTGACTCTGC CTGTGTTGAG CGGATGGGAG TGTGAAGTCA ACGCTCCTCT CGAACACACA 
TCATATTTAC TCCAAGGGAC AGAGATTATG GCCGTTCTGG ACCAAGTAGC CTCCAGAGTG 
CCTTGGTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hif1al2 ( XM_005161350.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161407.1 Danio rerio hypoxia-inducible factor 1, alpha subunit, like 2 (hif1al2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa25967
Clone ID Related Accession (Same CDS sequence)	XM_005161350.4 , XM_005161350.3 , NM_001012371.2
Accession Version	XM_005161350.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	hypoxia-inducible factor 1, alpha subunit, like 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005161350.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCAA TGAATTTGGA AACAGGAGGA GAAGACTGCC ACGGAAAAGC TAGAAGAAAG 
CAAACATTCA TCGAGCAGAA GAAAAGGAGA GTCTGCACAG AATGGAGAAA AACATGTTCC 
CGTTTGGCAG CACAGAGCAG GAGGAAGAAG GAGAGTCAAC TGTTCAAAGA GTTAACTGCT 
CTTCTGCCTC TGGACTTGAG TATGGACGGT CAGCGAGACA AAGCTTCGGT AATACGTCTC 
ACAATTGCTT ACCTGCACCT GAGGGGTCTT TTGGACACCA CAGACTCCTT GATGAAACGT 
CCTTCACCAG GAGTGTCGGA TAAATCTCAT GAGAGGGAGT TGCTAGATTC TGCCCTCGGA 
GGGTTTGTGG TTTTGTTGTC TTTGAATGGC AAGGTCATCT TCGCCACTAA GGGCTTAACT 
ACCCACACAG GAATTAACCA GATGGATCTG ATCGGAAGGA GCTTGCTTGA GTTCTTGCAC 
CCATGCGATC AGATGGAGGT CAAAGACATC CTCACGAGAC TGATTGGGAA CCAAGAACCA 
CAAAAATGTG AAGTGTTCCT CAGGATAAAA AAGGCAATGA ACACGAAACT GACTGGTTGG 
AAAATTATTC AGTGTTCTGG AAAGAAAAGG TCATCTGCTA CACCTGGATC CAGTTGTCTG 
GTTCTTGAGT GCAGGGTTCT GCCCATTCAG GAGATCACAG AAGTGGATGA AGCATTGAAT 
GCATGTACAT TTATGAGCGT TCACAGCCCT GACATGACGT TCACCTACTG CCACTCAAGG 
GTCGTGAAAT TGATCGGCTT TCGAGACACA GATCTGTTGG GTCAATCTGT ATACCAGTAC 
TATCATCCAT CTGACTGCCA GCAAATCCGC AAGGCACACA TTTGCTTGCT CTCCAAGGGT 
CAAGTGTCGA CCGGGAAATA CCGGCTGCTG CACAGATATG GAGGTTATGT GTGGGCAGAA 
ACAGACGCCT CACTGGTGTG CAACAGTCAG ACTGGTGTGC CAGAGAGTGT CGTCTGCATC 
AATTACATTC TAAGTGAAGT AGAGCAGCCC AATTTGCCAT TTTTACTGGA GAAGACAGAA 
CAACTTCTGA AACCAACATC TAGCTCCTAT TGTCCAGAGA CAGAACCTGC CCCTTTTCCG 
CCATCTAGAG GCCAGGAGGG TGAGAAAGAT CTCAAGGAAT CGAACGAACA CAACAGTTTT 
ATAACACATC TGAAACAAAG AGATGGTGAC ATACAGGAAG TTGAATGTAC AGAGATCCCA 
CATAACACAG AAGGGTGTTA TGATTTCAAT TGTGATCAGT GTGAGTTAGA CTTGGACAGT 
CTTGCTCCAT ACATTCCCAT GCACGGAGAG GACTTCCTGC TAACTCCCAT TATAGACAGG 
GCGATGGACA TGCCAGAATT AAACACGTGT AGACCATCAT GTAGCAGTCC ATCTCAAAAC 
CTGGATTCTT CAGAGGGAAC AACCGTTCTG CCAGTGAAGA GTCTTAATTC TACCCAACAA 
AGTGTCAGTA ATTCTTTTCA GACAAAAAGA ATCAGCGATA CTAACCTGAT ATCAACCAAG 
CAGTGGAGTG ACAGCTTTGA CCTTGTCAAC AAAGTTTCAA AGGTTTATGA TTACGATCCT 
AAACAACATA ATTATCAACT ATTTTCATCT GAAACCAAGG CAAATCATTG GGTGGAGGAG 
GCTCTGAAAT TTCCACAACC CAACAGAGTT CATTGGAACA TCGATGGATC AACACCACCA 
TGGATATGTG ACGGACACTT GAATCATCTT GAACCGAAGT TCAGATCACT TCCAGAGAAA 
ACCAATTGTC CTCCTCAAAA GAGAAGACAC CAACATGAAG TAAAAACAGC CGCGGCACTT 
TTGACTCTGC CTGTGTTGAG CGGATGGGAG TGTGAAGTCA ACGCTCCTCT CGAACACACA 
TCATATTTAC TCCAAGGGAC AGAGATTATG GCCGTTCTGG ACCAAGTAGC CTCCAGAGTG 
CCTTGGTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161407.1
CDS	425..2416
Translation	MTSMNLETGGEDCHGKARRKQTFIEQKKRRVCTEWRKTCSRLAAQSRRKKESQLFKELTALLPLDLSMDGQRDKASVIRLTIAYLHLRGLLDTTDSLMKRPSPGVSDKSHERELLDSALGGFVVLLSLNGKVIFATKGLTTHTGINQMDLIGRSLLEFLHPCDQMEVKDILTRLIGNQEPQKCEVFLRIKKAMNTKLTGWKIIQCSGKKRSSATPGSSCLVLECRVLPIQEITEVDEALNACTFMSVHSPDMTFTYCHSRVVKLIGFRDTDLLGQSVYQYYHPSDCQQIRKAHICLLSKGQVSTGKYRLLHRYGGYVWAETDASLVCNSQTGVPESVVCINYILSEVEQPNLPFLLEKTEQLLKPTSSSYCPETEPAPFPPSRGQEGEKDLKESNEHNSFITHLKQRDGDIQEVECTEIPHNTEGCYDFNCDQCELDLDSLAPYIPMHGEDFLLTPIIDRAMDMPELNTCRPSCSSPSQNLDSSEGTTVLPVKSLNSTQQSVSNSFQTKRISDTNLISTKQWSDSFDLVNKVSKVYDYDPKQHNYQLFSSETKANHWVEEALKFPQPNRVHWNIDGSTPPWICDGHLNHLEPKFRSLPEKTNCPPQKRRHQHEVKTAAALLTLPVLSGWECEVNAPLEHTSYLLQGTEIMAVLDQVASRVPWC

Target ORF information:

RefSeq Version	XM_005161350.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio hypoxia-inducible factor 1, alpha subunit, like 2 (hif1al2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161350.4

ORF Insert Sequence:

ATGACTTCAA TGAATTTGGA AACAGGAGGA GAAGACTGCC ACGGAAAAGC TAGAAGAAAG 
CAAACATTCA TCGAGCAGAA GAAAAGGAGA GTCTGCACAG AATGGAGAAA AACATGTTCC 
CGTTTGGCAG CACAGAGCAG GAGGAAGAAG GAGAGTCAAC TGTTCAAAGA GTTAACTGCT 
CTTCTGCCTC TGGACTTGAG TATGGACGGT CAGCGAGACA AAGCTTCGGT AATACGTCTC 
ACAATTGCTT ACCTGCACCT GAGGGGTCTT TTGGACACCA CAGACTCCTT GATGAAACGT 
CCTTCACCAG GAGTGTCGGA TAAATCTCAT GAGAGGGAGT TGCTAGATTC TGCCCTCGGA 
GGGTTTGTGG TTTTGTTGTC TTTGAATGGC AAGGTCATCT TCGCCACTAA GGGCTTAACT 
ACCCACACAG GAATTAACCA GATGGATCTG ATCGGAAGGA GCTTGCTTGA GTTCTTGCAC 
CCATGCGATC AGATGGAGGT CAAAGACATC CTCACGAGAC TGATTGGGAA CCAAGAACCA 
CAAAAATGTG AAGTGTTCCT CAGGATAAAA AAGGCAATGA ACACGAAACT GACTGGTTGG 
AAAATTATTC AGTGTTCTGG AAAGAAAAGG TCATCTGCTA CACCTGGATC CAGTTGTCTG 
GTTCTTGAGT GCAGGGTTCT GCCCATTCAG GAGATCACAG AAGTGGATGA AGCATTGAAT 
GCATGTACAT TTATGAGCGT TCACAGCCCT GACATGACGT TCACCTACTG CCACTCAAGG 
GTCGTGAAAT TGATCGGCTT TCGAGACACA GATCTGTTGG GTCAATCTGT ATACCAGTAC 
TATCATCCAT CTGACTGCCA GCAAATCCGC AAGGCACACA TTTGCTTGCT CTCCAAGGGT 
CAAGTGTCGA CCGGGAAATA CCGGCTGCTG CACAGATATG GAGGTTATGT GTGGGCAGAA 
ACAGACGCCT CACTGGTGTG CAACAGTCAG ACTGGTGTGC CAGAGAGTGT CGTCTGCATC 
AATTACATTC TAAGTGAAGT AGAGCAGCCC AATTTGCCAT TTTTACTGGA GAAGACAGAA 
CAACTTCTGA AACCAACATC TAGCTCCTAT TGTCCAGAGA CAGAACCTGC CCCTTTTCCG 
CCATCTAGAG GCCAGGAGGG TGAGAAAGAT CTCAAGGAAT CGAACGAACA CAACAGTTTT 
ATAACACATC TGAAACAAAG AGATGGTGAC ATACAGGAAG TTGAATGTAC AGAGATCCCA 
CATAACACAG AAGGGTGTTA TGATTTCAAT TGTGATCAGT GTGAGTTAGA CTTGGACAGT 
CTTGCTCCAT ACATTCCCAT GCACGGAGAG GACTTCCTGC TAACTCCCAT TATAGACAGG 
GCGATGGACA TGCCAGAATT AAACACGTGT AGACCATCAT GTAGCAGTCC ATCTCAAAAC 
CTGGATTCTT CAGAGGGAAC AACCGTTCTG CCAGTGAAGA GTCTTAATTC TACCCAACAA 
AGTGTCAGTA ATTCTTTTCA GACAAAAAGA ATCAGCGATA CTAACCTGAT ATCAACCAAG 
CAGTGGAGTG ACAGCTTTGA CCTTGTCAAC AAAGTTTCAA AGGTTTATGA TTACGATCCT 
AAACAACATA ATTATCAACT ATTTTCATCT GAAACCAAGG CAAATCATTG GGTGGAGGAG 
GCTCTGAAAT TTCCACAACC CAACAGAGTT CATTGGAACA TCGATGGATC AACACCACCA 
TGGATATGTG ACGGACACTT GAATCATCTT GAACCGAAGT TCAGATCACT TCCAGAGAAA 
ACCAATTGTC CTCCTCAAAA GAGAAGACAC CAACATGAAG TAAAAACAGC CGCGGCACTT 
TTGACTCTGC CTGTGTTGAG CGGATGGGAG TGTGAAGTCA ACGCTCCTCT CGAACACACA 
TCATATTTAC TCCAAGGGAC AGAGATTATG GCCGTTCTGG ACCAAGTAGC CTCCAGAGTG 
CCTTGGTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hif1al2 ( XM_005161350.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161407.1 Danio rerio hypoxia-inducible factor 1, alpha subunit, like 2 (hif1al2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa25967
Clone ID Related Accession (Same CDS sequence)	XM_005161350.4 , XM_005161350.3 , NM_001012371.2
Accession Version	XM_005161350.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	hypoxia-inducible factor 1, alpha subunit, like 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005161350.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCAA TGAATTTGGA AACAGGAGGA GAAGACTGCC ACGGAAAAGC TAGAAGAAAG 
CAAACATTCA TCGAGCAGAA GAAAAGGAGA GTCTGCACAG AATGGAGAAA AACATGTTCC 
CGTTTGGCAG CACAGAGCAG GAGGAAGAAG GAGAGTCAAC TGTTCAAAGA GTTAACTGCT 
CTTCTGCCTC TGGACTTGAG TATGGACGGT CAGCGAGACA AAGCTTCGGT AATACGTCTC 
ACAATTGCTT ACCTGCACCT GAGGGGTCTT TTGGACACCA CAGACTCCTT GATGAAACGT 
CCTTCACCAG GAGTGTCGGA TAAATCTCAT GAGAGGGAGT TGCTAGATTC TGCCCTCGGA 
GGGTTTGTGG TTTTGTTGTC TTTGAATGGC AAGGTCATCT TCGCCACTAA GGGCTTAACT 
ACCCACACAG GAATTAACCA GATGGATCTG ATCGGAAGGA GCTTGCTTGA GTTCTTGCAC 
CCATGCGATC AGATGGAGGT CAAAGACATC CTCACGAGAC TGATTGGGAA CCAAGAACCA 
CAAAAATGTG AAGTGTTCCT CAGGATAAAA AAGGCAATGA ACACGAAACT GACTGGTTGG 
AAAATTATTC AGTGTTCTGG AAAGAAAAGG TCATCTGCTA CACCTGGATC CAGTTGTCTG 
GTTCTTGAGT GCAGGGTTCT GCCCATTCAG GAGATCACAG AAGTGGATGA AGCATTGAAT 
GCATGTACAT TTATGAGCGT TCACAGCCCT GACATGACGT TCACCTACTG CCACTCAAGG 
GTCGTGAAAT TGATCGGCTT TCGAGACACA GATCTGTTGG GTCAATCTGT ATACCAGTAC 
TATCATCCAT CTGACTGCCA GCAAATCCGC AAGGCACACA TTTGCTTGCT CTCCAAGGGT 
CAAGTGTCGA CCGGGAAATA CCGGCTGCTG CACAGATATG GAGGTTATGT GTGGGCAGAA 
ACAGACGCCT CACTGGTGTG CAACAGTCAG ACTGGTGTGC CAGAGAGTGT CGTCTGCATC 
AATTACATTC TAAGTGAAGT AGAGCAGCCC AATTTGCCAT TTTTACTGGA GAAGACAGAA 
CAACTTCTGA AACCAACATC TAGCTCCTAT TGTCCAGAGA CAGAACCTGC CCCTTTTCCG 
CCATCTAGAG GCCAGGAGGG TGAGAAAGAT CTCAAGGAAT CGAACGAACA CAACAGTTTT 
ATAACACATC TGAAACAAAG AGATGGTGAC ATACAGGAAG TTGAATGTAC AGAGATCCCA 
CATAACACAG AAGGGTGTTA TGATTTCAAT TGTGATCAGT GTGAGTTAGA CTTGGACAGT 
CTTGCTCCAT ACATTCCCAT GCACGGAGAG GACTTCCTGC TAACTCCCAT TATAGACAGG 
GCGATGGACA TGCCAGAATT AAACACGTGT AGACCATCAT GTAGCAGTCC ATCTCAAAAC 
CTGGATTCTT CAGAGGGAAC AACCGTTCTG CCAGTGAAGA GTCTTAATTC TACCCAACAA 
AGTGTCAGTA ATTCTTTTCA GACAAAAAGA ATCAGCGATA CTAACCTGAT ATCAACCAAG 
CAGTGGAGTG ACAGCTTTGA CCTTGTCAAC AAAGTTTCAA AGGTTTATGA TTACGATCCT 
AAACAACATA ATTATCAACT ATTTTCATCT GAAACCAAGG CAAATCATTG GGTGGAGGAG 
GCTCTGAAAT TTCCACAACC CAACAGAGTT CATTGGAACA TCGATGGATC AACACCACCA 
TGGATATGTG ACGGACACTT GAATCATCTT GAACCGAAGT TCAGATCACT TCCAGAGAAA 
ACCAATTGTC CTCCTCAAAA GAGAAGACAC CAACATGAAG TAAAAACAGC CGCGGCACTT 
TTGACTCTGC CTGTGTTGAG CGGATGGGAG TGTGAAGTCA ACGCTCCTCT CGAACACACA 
TCATATTTAC TCCAAGGGAC AGAGATTATG GCCGTTCTGG ACCAAGTAGC CTCCAGAGTG 
CCTTGGTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161407.1
CDS	380..2371
Translation	MTSMNLETGGEDCHGKARRKQTFIEQKKRRVCTEWRKTCSRLAAQSRRKKESQLFKELTALLPLDLSMDGQRDKASVIRLTIAYLHLRGLLDTTDSLMKRPSPGVSDKSHERELLDSALGGFVVLLSLNGKVIFATKGLTTHTGINQMDLIGRSLLEFLHPCDQMEVKDILTRLIGNQEPQKCEVFLRIKKAMNTKLTGWKIIQCSGKKRSSATPGSSCLVLECRVLPIQEITEVDEALNACTFMSVHSPDMTFTYCHSRVVKLIGFRDTDLLGQSVYQYYHPSDCQQIRKAHICLLSKGQVSTGKYRLLHRYGGYVWAETDASLVCNSQTGVPESVVCINYILSEVEQPNLPFLLEKTEQLLKPTSSSYCPETEPAPFPPSRGQEGEKDLKESNEHNSFITHLKQRDGDIQEVECTEIPHNTEGCYDFNCDQCELDLDSLAPYIPMHGEDFLLTPIIDRAMDMPELNTCRPSCSSPSQNLDSSEGTTVLPVKSLNSTQQSVSNSFQTKRISDTNLISTKQWSDSFDLVNKVSKVYDYDPKQHNYQLFSSETKANHWVEEALKFPQPNRVHWNIDGSTPPWICDGHLNHLEPKFRSLPEKTNCPPQKRRHQHEVKTAAALLTLPVLSGWECEVNAPLEHTSYLLQGTEIMAVLDQVASRVPWC

Target ORF information:

RefSeq Version	XM_005161350.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio hypoxia-inducible factor 1, alpha subunit, like 2 (hif1al2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161350.3

ORF Insert Sequence:

ATGACTTCAA TGAATTTGGA AACAGGAGGA GAAGACTGCC ACGGAAAAGC TAGAAGAAAG 
CAAACATTCA TCGAGCAGAA GAAAAGGAGA GTCTGCACAG AATGGAGAAA AACATGTTCC 
CGTTTGGCAG CACAGAGCAG GAGGAAGAAG GAGAGTCAAC TGTTCAAAGA GTTAACTGCT 
CTTCTGCCTC TGGACTTGAG TATGGACGGT CAGCGAGACA AAGCTTCGGT AATACGTCTC 
ACAATTGCTT ACCTGCACCT GAGGGGTCTT TTGGACACCA CAGACTCCTT GATGAAACGT 
CCTTCACCAG GAGTGTCGGA TAAATCTCAT GAGAGGGAGT TGCTAGATTC TGCCCTCGGA 
GGGTTTGTGG TTTTGTTGTC TTTGAATGGC AAGGTCATCT TCGCCACTAA GGGCTTAACT 
ACCCACACAG GAATTAACCA GATGGATCTG ATCGGAAGGA GCTTGCTTGA GTTCTTGCAC 
CCATGCGATC AGATGGAGGT CAAAGACATC CTCACGAGAC TGATTGGGAA CCAAGAACCA 
CAAAAATGTG AAGTGTTCCT CAGGATAAAA AAGGCAATGA ACACGAAACT GACTGGTTGG 
AAAATTATTC AGTGTTCTGG AAAGAAAAGG TCATCTGCTA CACCTGGATC CAGTTGTCTG 
GTTCTTGAGT GCAGGGTTCT GCCCATTCAG GAGATCACAG AAGTGGATGA AGCATTGAAT 
GCATGTACAT TTATGAGCGT TCACAGCCCT GACATGACGT TCACCTACTG CCACTCAAGG 
GTCGTGAAAT TGATCGGCTT TCGAGACACA GATCTGTTGG GTCAATCTGT ATACCAGTAC 
TATCATCCAT CTGACTGCCA GCAAATCCGC AAGGCACACA TTTGCTTGCT CTCCAAGGGT 
CAAGTGTCGA CCGGGAAATA CCGGCTGCTG CACAGATATG GAGGTTATGT GTGGGCAGAA 
ACAGACGCCT CACTGGTGTG CAACAGTCAG ACTGGTGTGC CAGAGAGTGT CGTCTGCATC 
AATTACATTC TAAGTGAAGT AGAGCAGCCC AATTTGCCAT TTTTACTGGA GAAGACAGAA 
CAACTTCTGA AACCAACATC TAGCTCCTAT TGTCCAGAGA CAGAACCTGC CCCTTTTCCG 
CCATCTAGAG GCCAGGAGGG TGAGAAAGAT CTCAAGGAAT CGAACGAACA CAACAGTTTT 
ATAACACATC TGAAACAAAG AGATGGTGAC ATACAGGAAG TTGAATGTAC AGAGATCCCA 
CATAACACAG AAGGGTGTTA TGATTTCAAT TGTGATCAGT GTGAGTTAGA CTTGGACAGT 
CTTGCTCCAT ACATTCCCAT GCACGGAGAG GACTTCCTGC TAACTCCCAT TATAGACAGG 
GCGATGGACA TGCCAGAATT AAACACGTGT AGACCATCAT GTAGCAGTCC ATCTCAAAAC 
CTGGATTCTT CAGAGGGAAC AACCGTTCTG CCAGTGAAGA GTCTTAATTC TACCCAACAA 
AGTGTCAGTA ATTCTTTTCA GACAAAAAGA ATCAGCGATA CTAACCTGAT ATCAACCAAG 
CAGTGGAGTG ACAGCTTTGA CCTTGTCAAC AAAGTTTCAA AGGTTTATGA TTACGATCCT 
AAACAACATA ATTATCAACT ATTTTCATCT GAAACCAAGG CAAATCATTG GGTGGAGGAG 
GCTCTGAAAT TTCCACAACC CAACAGAGTT CATTGGAACA TCGATGGATC AACACCACCA 
TGGATATGTG ACGGACACTT GAATCATCTT GAACCGAAGT TCAGATCACT TCCAGAGAAA 
ACCAATTGTC CTCCTCAAAA GAGAAGACAC CAACATGAAG TAAAAACAGC CGCGGCACTT 
TTGACTCTGC CTGTGTTGAG CGGATGGGAG TGTGAAGTCA ACGCTCCTCT CGAACACACA 
TCATATTTAC TCCAAGGGAC AGAGATTATG GCCGTTCTGG ACCAAGTAGC CTCCAGAGTG 
CCTTGGTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hif1al2 ( XM_021468817.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324492.1 Danio rerio hypoxia-inducible factor 1, alpha subunit, like 2 (hif1al2), transcript variant X2, mRNA.

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CloneID	ODa150883
Clone ID Related Accession (Same CDS sequence)	XM_021468817.1
Accession Version	XM_021468817.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	hypoxia-inducible factor 1, alpha subunit, like 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGGTC AGCGAGACAA AGCTTCGGTA ATACGTCTCA CAATTGCTTA CCTGCACCTG 
AGGGGTCTTT TGGACACCAC AGACTCCTTG ATGAAACGTC CTTCACCAGG AGTGTCGGAT 
AAATCTCATG AGAGGGAGTT GCTAGATTCT GCCCTCGGAG GGTTTGTGGT TTTGTTGTCT 
TTGAATGGCA AGGTCATCTT CGCCACTAAG GGCTTAACTA CCCACACAGG AATTAACCAG 
ATGGATCTGA TCGGAAGGAG CTTGCTTGAG TTCTTGCACC CATGCGATCA GATGGAGGTC 
AAAGACATCC TCACGAGACT GATTGGGAAC CAAGAACCAC AAAAATGTGA AGTGTTCCTC 
AGGATAAAAA AGGCAATGAA CACGAAACTG ACTGGTTGGA AAATTATTCA GTGTTCTGGA 
AAGAAAAGGT CATCTGCTAC ACCTGGATCC AGTTGTCTGG TTCTTGAGTG CAGGGTTCTG 
CCCATTCAGG AGATCACAGA AGTGGATGAA GCATTGAATG CATGTACATT TATGAGCGTT 
CACAGCCCTG ACATGACGTT CACCTACTGC CACTCAAGGG TCGTGAAATT GATCGGCTTT 
CGAGACACAG ATCTGTTGGG TCAATCTGTA TACCAGTACT ATCATCCATC TGACTGCCAG 
CAAATCCGCA AGGCACACAT TTGCTTGCTC TCCAAGGGTC AAGTGTCGAC CGGGAAATAC 
CGGCTGCTGC ACAGATATGG AGGTTATGTG TGGGCAGAAA CAGACGCCTC ACTGGTGTGC 
AACAGTCAGA CTGGTGTGCC AGAGAGTGTC GTCTGCATCA ATTACATTCT AAGTGAAGTA 
GAGCAGCCCA ATTTGCCATT TTTACTGGAG AAGACAGAAC AACTTCTGAA ACCAACATCT 
AGCTCCTATT GTCCAGAGAC AGAACCTGCC CCTTTTCCGC CATCTAGAGG CCAGGAGGGT 
GAGAAAGATC TCAAGGAATC GAACGAACAC AACAGTTTTA TAACACATCT GAAACAAAGA 
GATGGTGACA TACAGGAAGT TGAATGTACA GAGATCCCAC ATAACACAGA AGGGTGTTAT 
GATTTCAATT GTGATCAGTG TGAGTTAGAC TTGGACAGTC TTGCTCCATA CATTCCCATG 
CACGGAGAGG ACTTCCTGCT AACTCCCATT ATAGACAGGG CGATGGACAT GCCAGAATTA 
AACACGTGTA GACCATCATG TAGCAGTCCA TCTCAAAACC TGGATTCTTC AGAGGGAACA 
ACCGTTCTGC CAGTGAAGAG TCTTAATTCT ACCCAACAAA GTGTCAGTAA TTCTTTTCAG 
ACAAAAAGAA TCAGCGATAC TAACCTGATA TCAACCAAGC AGTGGAGTGA CAGCTTTGAC 
CTTGTCAACA AAGTTTCAAA GGTTTATGAT TACGATCCTA AACAACATAA TTATCAACTA 
TTTTCATCTG AAACCAAGGC AAATCATTGG GTGGAGGAGG CTCTGAAATT TCCACAACCC 
AACAGAGTTC ATTGGAACAT CGATGGATCA ACACCACCAT GGATATGTGA CGGACACTTG 
AATCATCTTG AACCGAAGTT CAGATCACTT CCAGAGAAAA CCAATTGTCC TCCTCAAAAG 
AGAAGACACC AACATGAAGT AAAAACAGCC GCGGCACTTT TGACTCTGCC TGTGTTGAGC 
GGATGGGAGT GTGAAGTCAA CGCTCCTCTC GAACACACAT CATATTTACT CCAAGGGACA 
GAGATTATGG CCGTTCTGGA CCAAGTAGCC TCCAGAGTGC CTTGGTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324492.1
CDS	502..2292
Translation	MDGQRDKASVIRLTIAYLHLRGLLDTTDSLMKRPSPGVSDKSHERELLDSALGGFVVLLSLNGKVIFATKGLTTHTGINQMDLIGRSLLEFLHPCDQMEVKDILTRLIGNQEPQKCEVFLRIKKAMNTKLTGWKIIQCSGKKRSSATPGSSCLVLECRVLPIQEITEVDEALNACTFMSVHSPDMTFTYCHSRVVKLIGFRDTDLLGQSVYQYYHPSDCQQIRKAHICLLSKGQVSTGKYRLLHRYGGYVWAETDASLVCNSQTGVPESVVCINYILSEVEQPNLPFLLEKTEQLLKPTSSSYCPETEPAPFPPSRGQEGEKDLKESNEHNSFITHLKQRDGDIQEVECTEIPHNTEGCYDFNCDQCELDLDSLAPYIPMHGEDFLLTPIIDRAMDMPELNTCRPSCSSPSQNLDSSEGTTVLPVKSLNSTQQSVSNSFQTKRISDTNLISTKQWSDSFDLVNKVSKVYDYDPKQHNYQLFSSETKANHWVEEALKFPQPNRVHWNIDGSTPPWICDGHLNHLEPKFRSLPEKTNCPPQKRRHQHEVKTAAALLTLPVLSGWECEVNAPLEHTSYLLQGTEIMAVLDQVASRVPWC

Target ORF information:

RefSeq Version	XM_021468817.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio hypoxia-inducible factor 1, alpha subunit, like 2 (hif1al2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468817.1

ORF Insert Sequence:

ATGGACGGTC AGCGAGACAA AGCTTCGGTA ATACGTCTCA CAATTGCTTA CCTGCACCTG 
AGGGGTCTTT TGGACACCAC AGACTCCTTG ATGAAACGTC CTTCACCAGG AGTGTCGGAT 
AAATCTCATG AGAGGGAGTT GCTAGATTCT GCCCTCGGAG GGTTTGTGGT TTTGTTGTCT 
TTGAATGGCA AGGTCATCTT CGCCACTAAG GGCTTAACTA CCCACACAGG AATTAACCAG 
ATGGATCTGA TCGGAAGGAG CTTGCTTGAG TTCTTGCACC CATGCGATCA GATGGAGGTC 
AAAGACATCC TCACGAGACT GATTGGGAAC CAAGAACCAC AAAAATGTGA AGTGTTCCTC 
AGGATAAAAA AGGCAATGAA CACGAAACTG ACTGGTTGGA AAATTATTCA GTGTTCTGGA 
AAGAAAAGGT CATCTGCTAC ACCTGGATCC AGTTGTCTGG TTCTTGAGTG CAGGGTTCTG 
CCCATTCAGG AGATCACAGA AGTGGATGAA GCATTGAATG CATGTACATT TATGAGCGTT 
CACAGCCCTG ACATGACGTT CACCTACTGC CACTCAAGGG TCGTGAAATT GATCGGCTTT 
CGAGACACAG ATCTGTTGGG TCAATCTGTA TACCAGTACT ATCATCCATC TGACTGCCAG 
CAAATCCGCA AGGCACACAT TTGCTTGCTC TCCAAGGGTC AAGTGTCGAC CGGGAAATAC 
CGGCTGCTGC ACAGATATGG AGGTTATGTG TGGGCAGAAA CAGACGCCTC ACTGGTGTGC 
AACAGTCAGA CTGGTGTGCC AGAGAGTGTC GTCTGCATCA ATTACATTCT AAGTGAAGTA 
GAGCAGCCCA ATTTGCCATT TTTACTGGAG AAGACAGAAC AACTTCTGAA ACCAACATCT 
AGCTCCTATT GTCCAGAGAC AGAACCTGCC CCTTTTCCGC CATCTAGAGG CCAGGAGGGT 
GAGAAAGATC TCAAGGAATC GAACGAACAC AACAGTTTTA TAACACATCT GAAACAAAGA 
GATGGTGACA TACAGGAAGT TGAATGTACA GAGATCCCAC ATAACACAGA AGGGTGTTAT 
GATTTCAATT GTGATCAGTG TGAGTTAGAC TTGGACAGTC TTGCTCCATA CATTCCCATG 
CACGGAGAGG ACTTCCTGCT AACTCCCATT ATAGACAGGG CGATGGACAT GCCAGAATTA 
AACACGTGTA GACCATCATG TAGCAGTCCA TCTCAAAACC TGGATTCTTC AGAGGGAACA 
ACCGTTCTGC CAGTGAAGAG TCTTAATTCT ACCCAACAAA GTGTCAGTAA TTCTTTTCAG 
ACAAAAAGAA TCAGCGATAC TAACCTGATA TCAACCAAGC AGTGGAGTGA CAGCTTTGAC 
CTTGTCAACA AAGTTTCAAA GGTTTATGAT TACGATCCTA AACAACATAA TTATCAACTA 
TTTTCATCTG AAACCAAGGC AAATCATTGG GTGGAGGAGG CTCTGAAATT TCCACAACCC 
AACAGAGTTC ATTGGAACAT CGATGGATCA ACACCACCAT GGATATGTGA CGGACACTTG 
AATCATCTTG AACCGAAGTT CAGATCACTT CCAGAGAAAA CCAATTGTCC TCCTCAAAAG 
AGAAGACACC AACATGAAGT AAAAACAGCC GCGGCACTTT TGACTCTGCC TGTGTTGAGC 
GGATGGGAGT GTGAAGTCAA CGCTCCTCTC GAACACACAT CATATTTACT CCAAGGGACA 
GAGATTATGG CCGTTCTGGA CCAAGTAGCC TCCAGAGTGC CTTGGTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Age-dependent increase of oxidative stress regulates microRNA-29 family preserving cardiac health.
Scientific reports7(1)16839(2017 Dec)
Heid J,Cencioni C,Ripa R,Baumgart M,Atlante S,Milano G,Scopece A,Kuenne C,Guenther S,Azzimato V,Farsetti A,Rossi G,Braun T,Pompilio G,Martelli F,Zeiher AM,Cellerino A,Gaetano C,Spallotta F

Carbon nanopowder acts as a Trojan-horse for benzo(?)pyrene in Danio rerio embryos.
Nanotoxicology11(3)371-381(2017 Apr)
Binelli A,Del Giacco L,Santo N,Bini L,Magni S,Parolini M,Madaschi L,Ghilardi A,Maggioni D,Ascagni M,Armini A,Prosperi L,Landi C,La Porta C,Della Torre C

Foxa2 and Hif1ab regulate maturation of intestinal goblet cells by modulating agr2 expression in zebrafish embryos.
The Biochemical journal473(14)2205-18(2016 07)
Lai YR,Lu YF,Lien HW,Huang CJ,Hwang SP

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Phenotype anchoring in zebrafish reveals a potential role for matrix metalloproteinases (MMPs) in tamoxifen's effects on skin epithelium.
Toxicology and applied pharmacology29631-41(2016 Apr)
Bugel SM,Wehmas LC,La Du JK,Tanguay RL

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hif1al2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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