six1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	six1a
Entrez Gene ID	494168
Full Name	SIX homeobox 1a
Synonyms	six1b,six2,zgc:92332
General protein information	Preferred Names SIX homeobox 1a Names homeobox protein six1a homeobox protein six1b homeodomain transcription factor Six1b sine oculis homeobox homolog 1a sine oculis homeobox homolog 1b sine oculis homeobox homolog 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021480569.1	XP_021336244.1	homeobox protein six1a isoform X1
NM_001009904.1	NP_001009904.1	homeobox protein six1a

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Homeobox transcription factor Six7 governs expression of green opsin genes in zebrafish.
Ogawa Y, Shiraki T, Kojima D, Fukada Y
Proceedings. Biological sciences282(1812)20150659(2015 Aug)

Evolutionary comparison reveals that diverging CTCF sites are signatures of ancestral topological associating domains borders.
G?mez-Mar?n C, Tena JJ, Acemel RD, L?pez-Mayorga M, Naranjo S, de la Calle-Mustienes E, Maeso I, Beccari L, Aneas I, Vielmas E, Bovolenta P, Nobrega MA, Carvajal J, G?mez-Skarmeta JL
Proceedings of the National Academy of Sciences of the United States of America112(24)7542-7(2015 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Balancing cell numbers during organogenesis: Six1a differentially affects neurons and sensory hair cells in the inner ear
Title: Balancing cell numbers during organogenesis: Six1a differentially affects neurons and sensory hair cells in the inner ear.

six1a and pax3 do not function in the same regulatory network. We proposed four putative regulatory pathways to understand how six1a distinctly interacts with either myf5 or myod during zebrafish craniofacial muscle development.
Title: The transcription factor Six1a plays an essential role in the craniofacial myogenesis of zebrafish.

Six1a plays an essential role at the onset of fast muscle differentiation.
Title: Six1a is required for the onset of fast muscle differentiation in zebrafish.

The following six1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the six1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa02841	NM_001009904.1 Latest version!	Danio rerio SIX homeobox 1a (six1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
ODa148510	XM_021480569.1 Latest version!	Danio rerio SIX homeobox 1a (six1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

six1a ( NM_001009904.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001009904.1 Danio rerio SIX homeobox 1a (six1a), mRNA.

Price & Availability↑

CloneID	ODa02841
Clone ID Related Accession (Same CDS sequence)	NM_001009904.1
Accession Version	NM_001009904.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Danio rerio(zebrafish)
Product	homeobox protein six1a
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC076015.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC076015.1, EH451622.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505381, SAMEA3505392 [ECO:0000348] ##Evidence-Data-END##

ATGTCAATCT TGCCCTCGTT CGGCTTTACG CAAGAGCAAG TCGCGTGCGT CTGCGAGGTG 
CTGCAGCAGG GTGGGAACCT GGAGAGGCTC GGACGGTTCC TTTGGTCCCT GCCAGCCTGC 
GACCACCTCC ACAAGAACGA ATCCGTGCTC AAAGCGAAGG CTGTGGTTGC CTTCCACCGG 
GGAAACTTCC GTGAGCTCTA CAAGATCTTG GAGAGCTACC AGTTCTCCAC GCACAACCAC 
CCGAAGATGC AGCAGCTGTG GCTGAAGGCG CACTATGTTG AGGCGGAAAA GTTGCGGGGG 
CGCCCGCTAG GCGCTGTGGG CAAATATCGT GTGCGCAGGA AATTCCCTTT GCCCCGTACT 
ATCTGGGACG GCGAGGAAAC CAGTTATTGT TTTAAGGAAA AGTCTCGGAG CGTGCTGCGT 
GAATGGTACA CGCACAACCC TTATCCATCT CCACGGGAGA AGCGAGAGCT GGCAGAGGCA 
ACAGGACTGA CCACTACACA GGTCAGCAAC TGGTTTAAAA ACAGGAGACA GAGAGACCGG 
GCAGCCGAGG CAAAAGAGAG AGAAAACGGC GAGAACAACG GCGTCGGCGG AAAGCAGAGC 
CAGCGGTCTC CGCTCGACGG AGTGAAGTCG CTGATGTCGA GCTCGGAGGA TGAGTTTTCT 
CCGCCGCAGA GCCCCGAGCA CAGCTCCGTG CTTCTTCTGC AAGGCACCAT GAACAATCCC 
GGGGCTCCCG CATACCCAAT GCCCGGCCTG GGTGCGCCGC ACCCGCTACA CGGCATGCAA 
GGGCATCCGC ATCAGATCCA GGATTCACTA TTAGGATCTC TAACATCCAG CCTTGTGGAT 
TTAGGATCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001009904.1
CDS	237..1088
Translation	MSILPSFGFTQEQVACVCEVLQQGGNLERLGRFLWSLPACDHLHKNESVLKAKAVVAFHRGNFRELYKILESYQFSTHNHPKMQQLWLKAHYVEAEKLRGRPLGAVGKYRVRRKFPLPRTIWDGEETSYCFKEKSRSVLREWYTHNPYPSPREKRELAEATGLTTTQVSNWFKNRRQRDRAAEAKERENGENNGVGGKQSQRSPLDGVKSLMSSSEDEFSPPQSPEHSSVLLLQGTMNNPGAPAYPMPGLGAPHPLHGMQGHPHQIQDSLLGSLTSSLVDLGS

Target ORF information:

RefSeq Version	NM_001009904.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SIX homeobox 1a (six1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001009904.1

ORF Insert Sequence:

ATGTCAATCT TGCCCTCGTT CGGCTTTACG CAAGAGCAAG TCGCGTGCGT CTGCGAGGTG 
CTGCAGCAGG GTGGGAACCT GGAGAGGCTC GGACGGTTCC TTTGGTCCCT GCCAGCCTGC 
GACCACCTCC ACAAGAACGA ATCCGTGCTC AAAGCGAAGG CTGTGGTTGC CTTCCACCGG 
GGAAACTTCC GTGAGCTCTA CAAGATCTTG GAGAGCTACC AGTTCTCCAC GCACAACCAC 
CCGAAGATGC AGCAGCTGTG GCTGAAGGCG CACTATGTTG AGGCGGAAAA GTTGCGGGGG 
CGCCCGCTAG GCGCTGTGGG CAAATATCGT GTGCGCAGGA AATTCCCTTT GCCCCGTACT 
ATCTGGGACG GCGAGGAAAC CAGTTATTGT TTTAAGGAAA AGTCTCGGAG CGTGCTGCGT 
GAATGGTACA CGCACAACCC TTATCCATCT CCACGGGAGA AGCGAGAGCT GGCAGAGGCA 
ACAGGACTGA CCACTACACA GGTCAGCAAC TGGTTTAAAA ACAGGAGACA GAGAGACCGG 
GCAGCCGAGG CAAAAGAGAG AGAAAACGGC GAGAACAACG GCGTCGGCGG AAAGCAGAGC 
CAGCGGTCTC CGCTCGACGG AGTGAAGTCG CTGATGTCGA GCTCGGAGGA TGAGTTTTCT 
CCGCCGCAGA GCCCCGAGCA CAGCTCCGTG CTTCTTCTGC AAGGCACCAT GAACAATCCC 
GGGGCTCCCG CATACCCAAT GCCCGGCCTG GGTGCGCCGC ACCCGCTACA CGGCATGCAA 
GGGCATCCGC ATCAGATCCA GGATTCACTA TTAGGATCTC TAACATCCAG CCTTGTGGAT 
TTAGGATCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

six1a ( XM_021480569.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336244.1 Danio rerio SIX homeobox 1a (six1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa148510
Clone ID Related Accession (Same CDS sequence)	XM_021480569.1
Accession Version	XM_021480569.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	homeobox protein six1a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAATCT TGCCCTCGTT CGGCTTTACG CAAGAGCAAG TCGCGTGCGT CTGCGAGGTG 
CTGCAGCAGG GTGGGAACCT GGAGAGGCTC GGACGGTTCC TTTGGTCCCT GCCAGCCTGC 
GACCACCTCC ACAAGAACGA ATCCGTGCTC AAAGCGAAGG CTGTGGTTGC CTTCCACCGG 
GGAAACTTCC GTGAGCTCTA CAAGATCTTG GAGAGCTACC AGTTCTCCAC GCACAACCAC 
CCGAAGATGC AGCAGCTGTG GCTGAAGGCG CACTATGTTG AGGCGGAAAA GTTGCGGGGG 
CGCCCGCTAG GCGCTGTGGG CAAATATCGT GTGCGCAGGA AATTCCCTTT GCCCCGTACT 
ATCTGGGACG GCGAGGAGAC CAGTTATTGC TTTAAGGAAA AGTCTCGGAG CGTGCTGCGT 
GAATGGTACA CGCACAACCC TTATCCATCT CCACGGGAGA AGCGAGAGCT GGCAGAGGCA 
ACAGGACTGA CCACTACACA GGTCAGCAAC TGGTTTAAAA ACAGGAGACA GAGAGACCGG 
GCAGCCGAGG CAAAAGAGAG AGAAAACGGC GAGAACAACG GCGTCGGCGG AAAGCAGAGC 
CAGCGGTCTC CGCTCGACGG AGTGAAGTCG CTGATGTCGA GCTCGGAGGA TGAGTTTTCT 
CCGCCGCAGA GCCCCGAGCA CAGCTCCGTG CTTCTTCTGC AAGGCACCAT GAACAATCCC 
GCGGCTCCCG CATACCCAAT GCCCGGCCTG GGTGCGCCGC ACCCGCTACA CGGCATGCAA 
GGGCATCCGC ATCAGATCCA GGATTCACTG TTAGGATCTC TAACATCCAG CCTTGTGGAT 
TTAGGATCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336244.1
CDS	538..1389
Translation	MSILPSFGFTQEQVACVCEVLQQGGNLERLGRFLWSLPACDHLHKNESVLKAKAVVAFHRGNFRELYKILESYQFSTHNHPKMQQLWLKAHYVEAEKLRGRPLGAVGKYRVRRKFPLPRTIWDGEETSYCFKEKSRSVLREWYTHNPYPSPREKRELAEATGLTTTQVSNWFKNRRQRDRAAEAKERENGENNGVGGKQSQRSPLDGVKSLMSSSEDEFSPPQSPEHSSVLLLQGTMNNPAAPAYPMPGLGAPHPLHGMQGHPHQIQDSLLGSLTSSLVDLGS

Target ORF information:

RefSeq Version	XM_021480569.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SIX homeobox 1a (six1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480569.1

ORF Insert Sequence:

ATGTCAATCT TGCCCTCGTT CGGCTTTACG CAAGAGCAAG TCGCGTGCGT CTGCGAGGTG 
CTGCAGCAGG GTGGGAACCT GGAGAGGCTC GGACGGTTCC TTTGGTCCCT GCCAGCCTGC 
GACCACCTCC ACAAGAACGA ATCCGTGCTC AAAGCGAAGG CTGTGGTTGC CTTCCACCGG 
GGAAACTTCC GTGAGCTCTA CAAGATCTTG GAGAGCTACC AGTTCTCCAC GCACAACCAC 
CCGAAGATGC AGCAGCTGTG GCTGAAGGCG CACTATGTTG AGGCGGAAAA GTTGCGGGGG 
CGCCCGCTAG GCGCTGTGGG CAAATATCGT GTGCGCAGGA AATTCCCTTT GCCCCGTACT 
ATCTGGGACG GCGAGGAGAC CAGTTATTGC TTTAAGGAAA AGTCTCGGAG CGTGCTGCGT 
GAATGGTACA CGCACAACCC TTATCCATCT CCACGGGAGA AGCGAGAGCT GGCAGAGGCA 
ACAGGACTGA CCACTACACA GGTCAGCAAC TGGTTTAAAA ACAGGAGACA GAGAGACCGG 
GCAGCCGAGG CAAAAGAGAG AGAAAACGGC GAGAACAACG GCGTCGGCGG AAAGCAGAGC 
CAGCGGTCTC CGCTCGACGG AGTGAAGTCG CTGATGTCGA GCTCGGAGGA TGAGTTTTCT 
CCGCCGCAGA GCCCCGAGCA CAGCTCCGTG CTTCTTCTGC AAGGCACCAT GAACAATCCC 
GCGGCTCCCG CATACCCAAT GCCCGGCCTG GGTGCGCCGC ACCCGCTACA CGGCATGCAA 
GGGCATCCGC ATCAGATCCA GGATTCACTG TTAGGATCTC TAACATCCAG CCTTGTGGAT 
TTAGGATCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Homeobox transcription factor Six7 governs expression of green opsin genes in zebrafish.
Proceedings. Biological sciences282(1812)20150659(2015 Aug)
Ogawa Y,Shiraki T,Kojima D,Fukada Y

Evolutionary comparison reveals that diverging CTCF sites are signatures of ancestral topological associating domains borders.
Proceedings of the National Academy of Sciences of the United States of America112(24)7542-7(2015 Jun)
G?mez-Mar?n C,Tena JJ,Acemel RD,L?pez-Mayorga M,Naranjo S,de la Calle-Mustienes E,Maeso I,Beccari L,Aneas I,Vielmas E,Bovolenta P,Nobrega MA,Carvajal J,G?mez-Skarmeta JL

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six1a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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